LOCUS       DAZGTR010000001       116468 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGTR010000001 DAZGTR010000000
VERSION     DAZGTR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 116468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 116468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..116468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.001"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            169..1026
                     /gene="focA"
                     /locus_tag="ACHLCY_000001"
     CDS             169..1026
                     /gene="focA"
                     /locus_tag="ACHLCY_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP0367339.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1081..3363
                     /gene="pflB"
                     /locus_tag="ACHLCY_000002"
     CDS             1081..3363
                     /gene="pflB"
                     /locus_tag="ACHLCY_000002"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0367340.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3555..4295
                     /gene="pflA"
                     /locus_tag="ACHLCY_000003"
     CDS             3555..4295
                     /gene="pflA"
                     /locus_tag="ACHLCY_000003"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP0367341.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4377..4967)
                     /gene="ycaK"
                     /locus_tag="ACHLCY_000004"
     CDS             complement(4377..4967)
                     /gene="ycaK"
                     /locus_tag="ACHLCY_000004"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFP0367342.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            5067..5975
                     /gene="ycaN"
                     /locus_tag="ACHLCY_000005"
     CDS             5067..5975
                     /gene="ycaN"
                     /locus_tag="ACHLCY_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0367343.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            complement(5976..7406)
                     /gene="ycaM"
                     /locus_tag="ACHLCY_000006"
     CDS             complement(5976..7406)
                     /gene="ycaM"
                     /locus_tag="ACHLCY_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0367344.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(7614..8762)
                     /gene="ycaD"
                     /locus_tag="ACHLCY_000007"
     CDS             complement(7614..8762)
                     /gene="ycaD"
                     /locus_tag="ACHLCY_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0367345.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            9077..9703
                     /gene="ycaC"
                     /locus_tag="ACHLCY_000008"
     CDS             9077..9703
                     /gene="ycaC"
                     /locus_tag="ACHLCY_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP0367346.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            complement(9739..10602)
                     /gene="dmsC"
                     /locus_tag="ACHLCY_000009"
     CDS             complement(9739..10602)
                     /gene="dmsC"
                     /locus_tag="ACHLCY_000009"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP0367347.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(10604..11221)
                     /gene="dmsB"
                     /locus_tag="ACHLCY_000010"
     CDS             complement(10604..11221)
                     /gene="dmsB"
                     /locus_tag="ACHLCY_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP0367348.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            complement(11232..13676)
                     /gene="dmsA"
                     /locus_tag="ACHLCY_000011"
     CDS             complement(11232..13676)
                     /gene="dmsA"
                     /locus_tag="ACHLCY_000011"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP0367349.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            complement(13915..15207)
                     /gene="serS"
                     /locus_tag="ACHLCY_000012"
     CDS             complement(13915..15207)
                     /gene="serS"
                     /locus_tag="ACHLCY_000012"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP0367350.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(15298..16641)
                     /gene="rarA"
                     /locus_tag="ACHLCY_000013"
                     /gene_synonym="mgsA"
     CDS             complement(15298..16641)
                     /gene="rarA"
                     /locus_tag="ACHLCY_000013"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP0367351.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            complement(16652..17263)
                     /gene="lolA"
                     /locus_tag="ACHLCY_000014"
     CDS             complement(16652..17263)
                     /gene="lolA"
                     /locus_tag="ACHLCY_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP0367352.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            complement(17418..21524)
                     /gene="ftsK"
                     /locus_tag="ACHLCY_000015"
     CDS             complement(17418..21524)
                     /gene="ftsK"
                     /locus_tag="ACHLCY_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP0367353.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAASTTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQSYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQQQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            complement(21659..22153)
                     /gene="lrp"
                     /locus_tag="ACHLCY_000016"
     CDS             complement(21659..22153)
                     /gene="lrp"
                     /locus_tag="ACHLCY_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP0367354.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            22697..23662
                     /gene="trxB"
                     /locus_tag="ACHLCY_000017"
     CDS             22697..23662
                     /gene="trxB"
                     /locus_tag="ACHLCY_000017"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP0367355.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            23785..25551
                     /gene="cydD"
                     /locus_tag="ACHLCY_000018"
     CDS             23785..25551
                     /gene="cydD"
                     /locus_tag="ACHLCY_000018"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP0367356.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            25552..27273
                     /gene="cydC"
                     /locus_tag="ACHLCY_000019"
     CDS             25552..27273
                     /gene="cydC"
                     /locus_tag="ACHLCY_000019"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP0367357.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            27315..28019
                     /gene="aat"
                     /locus_tag="ACHLCY_000020"
     CDS             27315..28019
                     /gene="aat"
                     /locus_tag="ACHLCY_000020"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP0367358.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            28304..28522
                     /gene="infA"
                     /locus_tag="ACHLCY_000021"
     CDS             28304..28522
                     /gene="infA"
                     /locus_tag="ACHLCY_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP0367359.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            28776..28863
                     /locus_tag="ACHLCY_000022"
     tRNA            28776..28863
                     /locus_tag="ACHLCY_000022"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28810..28812,aa:Ser,seq:gga)
     gene            complement(29207..31483)
                     /gene="clpA"
                     /locus_tag="ACHLCY_000023"
     CDS             complement(29207..31483)
                     /gene="clpA"
                     /locus_tag="ACHLCY_000023"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP0367360.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(31514..31834)
                     /gene="clpS"
                     /locus_tag="ACHLCY_000024"
     CDS             complement(31514..31834)
                     /gene="clpS"
                     /locus_tag="ACHLCY_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP0367361.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            complement(31933..32007)
                     /gene="yljB"
                     /locus_tag="ACHLCY_000025"
     CDS             complement(31933..32007)
                     /gene="yljB"
                     /locus_tag="ACHLCY_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP0367362.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            32157..32381
                     /gene="cspD"
                     /locus_tag="ACHLCY_000026"
     CDS             32157..32381
                     /gene="cspD"
                     /locus_tag="ACHLCY_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP0367363.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(32454..34400)
                     /gene="macB"
                     /locus_tag="ACHLCY_000027"
     CDS             complement(32454..34400)
                     /gene="macB"
                     /locus_tag="ACHLCY_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP0367364.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(34397..35512)
                     /gene="macA"
                     /locus_tag="ACHLCY_000028"
     CDS             complement(34397..35512)
                     /gene="macA"
                     /locus_tag="ACHLCY_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP0367365.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            35663..36619
                     /gene="ybjX"
                     /locus_tag="ACHLCY_000029"
     CDS             35663..36619
                     /gene="ybjX"
                     /locus_tag="ACHLCY_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP0367366.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(36616..38274)
                     /gene="ybjD"
                     /locus_tag="ACHLCY_000030"
     CDS             complement(36616..38274)
                     /gene="ybjD"
                     /locus_tag="ACHLCY_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP0367367.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            38700..39395
                     /gene="aqpZ"
                     /locus_tag="ACHLCY_000031"
     CDS             38700..39395
                     /gene="aqpZ"
                     /locus_tag="ACHLCY_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP0367368.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            39890..40789
                     /gene="lysO"
                     /locus_tag="ACHLCY_000032"
     CDS             39890..40789
                     /gene="lysO"
                     /locus_tag="ACHLCY_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP0367369.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            40933..42585
                     /gene="hcp"
                     /locus_tag="ACHLCY_000033"
                     /gene_synonym="priS"
     CDS             40933..42585
                     /gene="hcp"
                     /locus_tag="ACHLCY_000033"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP0367370.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            42597..43565
                     /gene="hcr"
                     /locus_tag="ACHLCY_000034"
     CDS             42597..43565
                     /gene="hcr"
                     /locus_tag="ACHLCY_000034"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP0367371.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            43698..45416
                     /gene="poxB"
                     /locus_tag="ACHLCY_000035"
     CDS             43698..45416
                     /gene="poxB"
                     /locus_tag="ACHLCY_000035"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP0367372.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHTPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            45453..46454
                     /gene="ltaE"
                     /locus_tag="ACHLCY_000036"
     CDS             45453..46454
                     /gene="ltaE"
                     /locus_tag="ACHLCY_000036"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP0367373.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            46465..47895
                     /gene="ybjT"
                     /locus_tag="ACHLCY_000037"
     CDS             46465..47895
                     /gene="ybjT"
                     /locus_tag="ACHLCY_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP0367374.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            47994..49007
                     /gene="ybjS"
                     /locus_tag="ACHLCY_000038"
     CDS             47994..49007
                     /gene="ybjS"
                     /locus_tag="ACHLCY_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0367375.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWTV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(49004..49834)
                     /gene="amiD"
                     /locus_tag="ACHLCY_000039"
     CDS             complement(49004..49834)
                     /gene="amiD"
                     /locus_tag="ACHLCY_000039"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP0367376.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLVKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(49831..50154)
                     /gene="ybjQ"
                     /locus_tag="ACHLCY_000040"
     CDS             complement(49831..50154)
                     /gene="ybjQ"
                     /locus_tag="ACHLCY_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP0367377.1"
                     /translation="MQFSTTPTLEGQIIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            50280..50795
                     /gene="ybjP"
                     /locus_tag="ACHLCY_000041"
     CDS             50280..50795
                     /gene="ybjP"
                     /locus_tag="ACHLCY_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0367378.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            51013..51741
                     /gene="artP"
                     /locus_tag="ACHLCY_000042"
     CDS             51013..51741
                     /gene="artP"
                     /locus_tag="ACHLCY_000042"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP0367379.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            51759..52490
                     /gene="artI"
                     /locus_tag="ACHLCY_000043"
     CDS             51759..52490
                     /gene="artI"
                     /locus_tag="ACHLCY_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP0367380.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            52497..53213
                     /gene="artQ"
                     /locus_tag="ACHLCY_000044"
     CDS             52497..53213
                     /gene="artQ"
                     /locus_tag="ACHLCY_000044"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP0367381.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            53213..53881
                     /gene="artM"
                     /locus_tag="ACHLCY_000045"
     CDS             53213..53881
                     /gene="artM"
                     /locus_tag="ACHLCY_000045"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP0367382.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            54174..54905
                     /gene="artJ"
                     /locus_tag="ACHLCY_000046"
     CDS             54174..54905
                     /gene="artJ"
                     /locus_tag="ACHLCY_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP0367383.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(55080..56207)
                     /gene="rlmC"
                     /locus_tag="ACHLCY_000047"
     CDS             complement(55080..56207)
                     /gene="rlmC"
                     /locus_tag="ACHLCY_000047"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP0367384.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(56248..56736)
                     /gene="ybjO"
                     /locus_tag="ACHLCY_000048"
     CDS             complement(56248..56736)
                     /gene="ybjO"
                     /locus_tag="ACHLCY_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP0367385.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(56796..57641)
                     /gene="potI"
                     /locus_tag="ACHLCY_000049"
     CDS             complement(56796..57641)
                     /gene="potI"
                     /locus_tag="ACHLCY_000049"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP0367386.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(57638..58591)
                     /gene="potH"
                     /locus_tag="ACHLCY_000050"
                     /pseudo
     CDS             complement(57638..58591)
                     /gene="potH"
                     /locus_tag="ACHLCY_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
     gene            complement(58601..59734)
                     /gene="potG"
                     /locus_tag="ACHLCY_000051"
     CDS             complement(58601..59734)
                     /gene="potG"
                     /locus_tag="ACHLCY_000051"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP0367387.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(59829..60941)
                     /gene="potF"
                     /locus_tag="ACHLCY_000052"
     CDS             complement(59829..60941)
                     /gene="potF"
                     /locus_tag="ACHLCY_000052"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP0367388.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVGAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(61292..61768)
                     /gene="ybjN"
                     /locus_tag="ACHLCY_000053"
     CDS             complement(61292..61768)
                     /gene="ybjN"
                     /locus_tag="ACHLCY_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP0367389.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQILMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(61856..62758)
                     /gene="rimK"
                     /locus_tag="ACHLCY_000054"
     CDS             complement(61856..62758)
                     /gene="rimK"
                     /locus_tag="ACHLCY_000054"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP0367390.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(62819..63541)
                     /gene="nfsA"
                     /locus_tag="ACHLCY_000055"
     CDS             complement(62819..63541)
                     /gene="nfsA"
                     /locus_tag="ACHLCY_000055"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP0367391.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(63525..63812)
                     /gene="ybjC"
                     /locus_tag="ACHLCY_000056"
     CDS             complement(63525..63812)
                     /gene="ybjC"
                     /locus_tag="ACHLCY_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP0367392.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            63972..64229
                     /gene="grxA"
                     /locus_tag="ACHLCY_000057"
     CDS             63972..64229
                     /gene="grxA"
                     /locus_tag="ACHLCY_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP0367393.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(64259..64636)
                     /gene="ybjM"
                     /locus_tag="ACHLCY_000058"
     CDS             complement(64259..64636)
                     /gene="ybjM"
                     /locus_tag="ACHLCY_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP0367394.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            64906..66591
                     /gene="ybjL"
                     /locus_tag="ACHLCY_000059"
     CDS             64906..66591
                     /gene="ybjL"
                     /locus_tag="ACHLCY_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP0367395.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(66766..67302)
                     /gene="rcdA"
                     /locus_tag="ACHLCY_000060"
     CDS             complement(66766..67302)
                     /gene="rcdA"
                     /locus_tag="ACHLCY_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP0367396.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            67386..68594
                     /gene="ybjJ"
                     /locus_tag="ACHLCY_000061"
                     /pseudo
     CDS             67386..68594
                     /gene="ybjJ"
                     /locus_tag="ACHLCY_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            68594..69409
                     /gene="ybjI"
                     /locus_tag="ACHLCY_000062"
     CDS             68594..69409
                     /gene="ybjI"
                     /locus_tag="ACHLCY_000062"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP0367397.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            69495..69779
                     /gene="ybjH"
                     /locus_tag="ACHLCY_000063"
     CDS             69495..69779
                     /gene="ybjH"
                     /locus_tag="ACHLCY_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP0367398.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(69820..71052)
                     /gene="mdfA"
                     /locus_tag="ACHLCY_000064"
     CDS             complement(69820..71052)
                     /gene="mdfA"
                     /locus_tag="ACHLCY_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP0367399.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            71337..71933
                     /gene="ybjG"
                     /locus_tag="ACHLCY_000065"
     CDS             71337..71933
                     /gene="ybjG"
                     /locus_tag="ACHLCY_000065"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0367400.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            71991..72749
                     /gene="deoR"
                     /locus_tag="ACHLCY_000066"
     CDS             71991..72749
                     /gene="deoR"
                     /locus_tag="ACHLCY_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP0367401.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(72796..73998)
                     /gene="dacC"
                     /locus_tag="ACHLCY_000067"
     CDS             complement(72796..73998)
                     /gene="dacC"
                     /locus_tag="ACHLCY_000067"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0367402.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            74245..74871
                     /gene="gstB"
                     /locus_tag="ACHLCY_000068"
     CDS             74245..74871
                     /gene="gstB"
                     /locus_tag="ACHLCY_000068"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP0367403.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(74868..75983)
                     /gene="yliI"
                     /locus_tag="ACHLCY_000069"
     CDS             complement(74868..75983)
                     /gene="yliI"
                     /locus_tag="ACHLCY_000069"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP0367404.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(76094..76477)
                     /gene="bssR"
                     /locus_tag="ACHLCY_000070"
     CDS             complement(76094..76477)
                     /gene="bssR"
                     /locus_tag="ACHLCY_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP0367405.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            76690..78015
                     /gene="rimO"
                     /locus_tag="ACHLCY_000071"
     CDS             76690..78015
                     /gene="rimO"
                     /locus_tag="ACHLCY_000071"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP0367406.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(78062..79390)
                     /gene="dgcI"
                     /locus_tag="ACHLCY_000072"
     CDS             complement(78062..79390)
                     /gene="dgcI"
                     /locus_tag="ACHLCY_000072"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0367407.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYYAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(79398..81755)
                     /gene="pdeI"
                     /locus_tag="ACHLCY_000073"
     CDS             complement(79398..81755)
                     /gene="pdeI"
                     /locus_tag="ACHLCY_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0367408.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLL
                     LCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSI
                     RDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEA
                     LCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLH
                     INVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQG
                     VRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPV
                     IAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(81933..82844)
                     /gene="gsiD"
                     /locus_tag="ACHLCY_000074"
     CDS             complement(81933..82844)
                     /gene="gsiD"
                     /locus_tag="ACHLCY_000074"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP0367409.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTISLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQSPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(82847..83767)
                     /gene="gsiC"
                     /locus_tag="ACHLCY_000075"
     CDS             complement(82847..83767)
                     /gene="gsiC"
                     /locus_tag="ACHLCY_000075"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP0367410.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETRV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(83785..85323)
                     /gene="gsiB"
                     /locus_tag="ACHLCY_000076"
     CDS             complement(83785..85323)
                     /gene="gsiB"
                     /locus_tag="ACHLCY_000076"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP0367411.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYIVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(85343..87214)
                     /gene="gsiA"
                     /locus_tag="ACHLCY_000077"
     CDS             complement(85343..87214)
                     /gene="gsiA"
                     /locus_tag="ACHLCY_000077"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP0367412.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(87201..88166)
                     /gene="iaaA"
                     /locus_tag="ACHLCY_000078"
     CDS             complement(87201..88166)
                     /gene="iaaA"
                     /locus_tag="ACHLCY_000078"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0367413.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            88370..89605
                     /gene="moeA"
                     /locus_tag="ACHLCY_000079"
     CDS             88370..89605
                     /gene="moeA"
                     /locus_tag="ACHLCY_000079"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP0367414.1"
                     /translation="MEFTTGLMSLDTALNEILSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            89605..90354
                     /gene="moeB"
                     /locus_tag="ACHLCY_000080"
     CDS             89605..90354
                     /gene="moeB"
                     /locus_tag="ACHLCY_000080"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP0367415.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(90430..91092)
                     /gene="fsa"
                     /locus_tag="ACHLCY_000081"
     CDS             complement(90430..91092)
                     /gene="fsa"
                     /locus_tag="ACHLCY_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0367416.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            91223..92122
                     /gene="ybiY"
                     /locus_tag="ACHLCY_000082"
     CDS             91223..92122
                     /gene="ybiY"
                     /locus_tag="ACHLCY_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP0367417.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            92128..94560
                     /gene="ybiW"
                     /locus_tag="ACHLCY_000083"
     CDS             92128..94560
                     /gene="ybiW"
                     /locus_tag="ACHLCY_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP0367418.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            94706..95521
                     /gene="ybiV"
                     /locus_tag="ACHLCY_000084"
     CDS             94706..95521
                     /gene="ybiV"
                     /locus_tag="ACHLCY_000084"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP0367419.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            95673..96938
                     /gene="ybiU"
                     /locus_tag="ACHLCY_000085"
     CDS             95673..96938
                     /gene="ybiU"
                     /locus_tag="ACHLCY_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP0367420.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(97175..98767)
                     /gene="ybiT"
                     /locus_tag="ACHLCY_000086"
     CDS             complement(97175..98767)
                     /gene="ybiT"
                     /locus_tag="ACHLCY_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP0367421.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            98986..99906
                     /gene="ldtB"
                     /locus_tag="ACHLCY_000087"
     CDS             98986..99906
                     /gene="ldtB"
                     /locus_tag="ACHLCY_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0367422.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKN
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(99965..101083)
                     /gene="ybiR"
                     /locus_tag="ACHLCY_000088"
     CDS             complement(99965..101083)
                     /gene="ybiR"
                     /locus_tag="ACHLCY_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP0367423.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(101080..101547)
                     /gene="mntR"
                     /locus_tag="ACHLCY_000089"
     CDS             complement(101080..101547)
                     /gene="mntR"
                     /locus_tag="ACHLCY_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP0367424.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            101733..101861
                     /gene="mntS"
                     /locus_tag="ACHLCY_000090"
     CDS             101733..101861
                     /gene="mntS"
                     /locus_tag="ACHLCY_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP0367425.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            102133..103716
                     /gene="opgE"
                     /locus_tag="ACHLCY_000091"
     CDS             102133..103716
                     /gene="opgE"
                     /locus_tag="ACHLCY_000091"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0367426.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(103765..104280)
                     /gene="ompX"
                     /locus_tag="ACHLCY_000092"
     CDS             complement(103765..104280)
                     /gene="ompX"
                     /locus_tag="ACHLCY_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP0367427.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(104333..104398)
                     /locus_tag="ACHLCY_000093"
     CDS             complement(104333..104398)
                     /locus_tag="ACHLCY_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367428.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            104633..105520
                     /gene="rhtA"
                     /locus_tag="ACHLCY_000094"
     CDS             104633..105520
                     /gene="rhtA"
                     /locus_tag="ACHLCY_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP0367429.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            105819..106322
                     /gene="dps"
                     /locus_tag="ACHLCY_000095"
                     /gene_synonym="pexB"
     CDS             105819..106322
                     /gene="dps"
                     /locus_tag="ACHLCY_000095"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP0367430.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            106726..107472
                     /gene="glnH"
                     /locus_tag="ACHLCY_000096"
     CDS             106726..107472
                     /gene="glnH"
                     /locus_tag="ACHLCY_000096"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP0367431.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            107611..108270
                     /gene="glnP"
                     /locus_tag="ACHLCY_000097"
     CDS             107611..108270
                     /gene="glnP"
                     /locus_tag="ACHLCY_000097"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP0367432.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVLYLIITLVLSFILRRLERRMKI
                     L"
     gene            108267..108989
                     /gene="glnQ"
                     /locus_tag="ACHLCY_000098"
     CDS             108267..108989
                     /gene="glnQ"
                     /locus_tag="ACHLCY_000098"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP0367433.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            109106..111331
                     /gene="ybiO"
                     /locus_tag="ACHLCY_000099"
     CDS             109106..111331
                     /gene="ybiO"
                     /locus_tag="ACHLCY_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP0367434.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(111328..112254)
                     /gene="rlmF"
                     /locus_tag="ACHLCY_000100"
     CDS             complement(111328..112254)
                     /gene="rlmF"
                     /locus_tag="ACHLCY_000100"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP0367435.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDELNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            112530..112790
                     /gene="mcbA"
                     /locus_tag="ACHLCY_000101"
     CDS             112530..112790
                     /gene="mcbA"
                     /locus_tag="ACHLCY_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP0367436.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            113054..115336
                     /gene="fiu"
                     /locus_tag="ACHLCY_000102"
     CDS             113054..115336
                     /gene="fiu"
                     /locus_tag="ACHLCY_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP0367437.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGVNANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSVNRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            115378..116055
                     /gene="ybiX"
                     /locus_tag="ACHLCY_000103"
     CDS             115378..116055
                     /gene="ybiX"
                     /locus_tag="ACHLCY_000103"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP0367438.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            116129..116395
                     /gene="ybiI"
                     /locus_tag="ACHLCY_000104"
     CDS             116129..116395
                     /gene="ybiI"
                     /locus_tag="ACHLCY_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP0367439.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
BASE COUNT        28442 a        30741 c        28828 g        28457 t
ORIGIN      
        1 atttttaaaa tttattttta tttggataat caaatattta ctccgtattt gcataaaaac
       61 catgcgagtt acgggcctat aagccaggcg agatatgatc tatatcaatt tctcatctat
      121 aatgctttgt tagtatctcg tcgccgactt aataaagaga gagttagtgt gaaagctgac
      181 aacccttttg atcttttact tcctgctgca atggccaaag tggccgaaga ggcgggtgtc
      241 tataaagcaa cgaaacatcc gcttaagact ttctatctgg cgattaccgc cggtgttttc
      301 atctcaatcg cattcgtctt ctatatcaca gcaaccactg gcacaggcac aatgcccttc
      361 ggcatggcaa aactggttgg cggcatttgc ttctctctgg ggctgattct ttgtgttgtc
      421 tgcggagccg atctctttac ttccacagtg ttgattgttg ttgctaaggc gagtgggcgc
      481 atcacctggg gtcagttggc gaaaaactgg ctaaatgtct attttggcaa cctggtcggc
      541 gcactgctgt ttgtactttt aatgtggctt tccggcgagt atatgaccgc aaatggtcaa
      601 tggggactaa acgtcctaca aaccgccgac cacaaagtgc accatacttt tattgaggcc
      661 gtctgccttg gtatcctggc aaacctgatg gtatgtctgg cagtatggat gagttattct
      721 ggccgtagcc tgatggacaa agcgttcatt atggtgctgc cggtcgcgat gtttgttgcc
      781 agcggttttg agcacagtat cgcaaacatg tttatgatcc cgatgggtat tgtaatccgc
      841 gacttcgcat ctccggaatt ctggaccgct gtcggttctg caccggaaaa tttttctcac
      901 ctgaccgtga tgaacttcat cactgataac ctgattccgg ttacgatcgg taatattatc
      961 ggcggtggtt tgttggttgg gttgacatac tgggtcattt acctgcgtga aaacgatcac
     1021 cattaatggt tgtcgaagta cgcagtaaat aaaaaatcca cttaagaagg taggtgttac
     1081 atgtccgagc ttaatgaaaa gttagccaca gcctgggaag gttttaccaa aggtgactgg
     1141 cagaatgaag taaacgtccg tgacttcatt cagaaaaact acactccgta cgagggtgac
     1201 gagtccttcc tggctggcgc tactgaagcg accaccaccc tgtgggacaa agtaatggaa
     1261 ggcgttaaac tggaaaaccg cactcacgcg ccagttgact ttgacaccgc tgttgcttcc
     1321 accatcacct ctcacgacgc tggctacatc aacaagcagc ttgagaaaat cgttggtctg
     1381 cagactgaag ctccgctgaa acgtgctctt atcccgttcg gtggtatcaa aatgatcgaa
     1441 ggttcctgca aagcgtacaa ccgcgaactg gatccgatga tcaaaaaaat cttcactgaa
     1501 taccgtaaaa ctcacaacca gggcgtgttc gacgtttaca ctccggacat cctgcgttgc
     1561 cgtaaatctg gtgttctgac cggtctgcca gatgcatatg gccgtggccg tatcatcggt
     1621 gactaccgtc gcgttgcgct gtacggtatc gactacctga tgaaagacaa actggcacag
     1681 ttcacttctc tgcaggctga tctggaaaac ggcgtaaacc tggaacagac tatccgtctg
     1741 cgcgaagaaa tcgctgaaca gcaccgcgct ctgggtcaga tgaaagaaat ggctgcgaaa
     1801 tacggctacg acatctctgg tccggctacc aacgctcagg aagctatcca gtggacttac
     1861 ttcggctacc tggctgctgt taagtctcag aacggtgctg caatgtcctt cggtcgtacc
     1921 tccaccttcc tggatgtgta catcgaacgt gacctgaaag ctggcaagat caccgaacaa
     1981 gaagcgcagg aaatggttga ccacctggtc atgaaactgc gtatggttcg cttcctgcgt
     2041 actccggaat acgatgaact gttctctggc gacccgatct gggcaaccga atctatcggt
     2101 ggtatgggcc tcgacggtcg taccctggtt accaaaaaca gcttccgttt cctgaacacc
     2161 ctgtacacca tgggtccgtc tccggaaccg aacatgacca ttctgtggtc tgaaaaactg
     2221 ccgctgaact tcaagaaatt cgccgctaaa gtgtccatcg acacctcttc tctgcagtat
     2281 gagaacgatg acctgatgcg tccggacttc aacaacgatg actacgctat tgcttgctgc
     2341 gtaagcccga tgatcgttgg taaacaaatg cagttcttcg gtgcgcgtgc aaacctggcg
     2401 aaaaccatgc tgtacgcaat caacggcggc gttgacgaaa aactgaaaat gcaggttggt
     2461 ccgaagtctg aaccgatcaa aggcgatgtc ctgaactatg atgaagtgat ggagcgcatg
     2521 gatcacttca tggactggct ggctaaacag tacatcactg cactgaacat catccactac
     2581 atgcacgaca agtacagcta cgaagcctct ctgatggcgc tgcacgaccg tgacgttatc
     2641 cgcaccatgg cgtgtggtat cgctggtctg tccgttgctg ctgactccct gtctgcaatc
     2701 aaatatgcga aagttaaacc gattcgtgac gaagacggtc tggctatcga cttcgaaatc
     2761 gaaggcgaat acccgcagtt tggtaacaat gatccgcgtg tagatgacct ggctgttgac
     2821 ctggtagaac gtttcatgaa gaaaattcag aaactgcaca cctaccgtga cgctatcccg
     2881 actcagtctg ttctgaccat cacttctaac gttgtgtatg gtaagaaaac tggtaacacc
     2941 ccagacggtc gtcgtgctgg cgcgccgttc ggaccgggtg ctaacccgat gcacggtcgt
     3001 gaccagaaag gtgctgtagc gtctctgact tccgttgcta aactgccgtt tgcttacgct
     3061 aaagatggta tctcctacac cttctctatc gttccgaacg cactgggtaa agacgacgaa
     3121 gttcgtaaga ccaacctggc tggtctgatg gatggttact tccaccacga agcatccatc
     3181 gaaggtggtc agcacctgaa cgttaacgtg atgaaccgtg aaatgctgct cgacgcgatg
     3241 gaaaacccgg aaaaatatcc gcagctgacc atccgtgtat ctggctacgc agtacgtttc
     3301 aactcgctga ctaaagaaca gcagcaggac gttattactc gtaccttcac tcaatctatg
     3361 taattagatt tgactgaaat cgtacagtaa aaagcgtaca ataaaggctc cacgaaagtg
     3421 gggccttttt tagcacgaga gccttttttg tcagctatct atactttaag gtgactgcca
     3481 aaacagactc gacgtagcct tcgagctgcg caccaacacg gcctcagatg ggccacatct
     3541 ggagaaacac cgcaatgtca gttattggtc gcattcactc ctttgaatcc tgtggaaccg
     3601 tagacggccc aggtattcgc tttatcacct ttttccaggg ctgcctgatg cgctgcctgt
     3661 attgtcataa ccgcgacacc tgggatacgc atggcggtaa agaagttacc gttgaagatt
     3721 taatgaaaga ggtggtgacc tatcgccact ttatgaacgc ttccggcggc ggcgttaccg
     3781 catccggcgg tgaggcaatc ctacaagctg agtttgttcg tgactggttc cgcgcctgca
     3841 aaaaagaagg cattcatacc tgtctggaca ccaacggttt tgttcgtcgt tacgatccgg
     3901 tgattgatga actgctggaa gtaaccgacc tggtaatgct cgatctcaaa cagatgaacg
     3961 acgagatcca ccaaaatctg gttggagttt ccaaccaccg cacgctggag ttcgccaaat
     4021 atctggcgaa caaaaatgtg aaggtgtgga tccgctacgt tgttgtccca ggctggtctg
     4081 acgatgacga ttcagcgcat cgcctcggtg aatttacccg tgatatgggc aacgttgaga
     4141 aaatcgaact cctcccctac cacgagctgg gcaaacacaa atgggtggca atgggtgaag
     4201 agtacaaact cgacggtgtt aaaccaccta agaaagagac catggaacgc gtgaaaggca
     4261 ttcttgagca gtacggtcat aaggtaatgt tctaatttcc ccgtaaagcg gcaactttat
     4321 tgagttgccg cttttttatt ccgcgcttca ttctgctaca gcgtttttaa cacattttat
     4381 agtgcatcta ccatatcccg tacctgagat aataacgact gataatagct cgcatgaagt
     4441 tcttgcccat cgaataccac tgtattacac aagaaagtga catcggcatc ttcaatacca
     4501 agataactgc acatattttt caaataatcg cttatatttt tttcccagcc catctggaca
     4561 aatgattctt tgtctcctcc aaccagcgcc acccaacgaa ctttattgaa tggtaatttg
     4621 tgcccatctc cataagccag cccattattc catactctgt caatatatcc ttttagcatt
     4681 gccgggaagc tataccacca gagaggaaaa accaccacta acgtgtcatg ttcaagcagc
     4741 tctgaataaa gctgatgaac ctctggagaa taacgtttat ccatattctt ccagtctggt
     4801 tcatcttccg gcgtcatcac tgggttgaaa ttacgccgat ataaatcgag ttccgtcacc
     4861 tgtattttcc gctccattgc ccgctgatgg atcgcatcag caatatttgc ggtcaatgaa
     4921 tcatgacgag gatgggccca taccaaataa atacgttcag actgcataat tttacctcca
     4981 cgttaagttc tggagcgcac tttacgcagg cggcataatc ctgtaaacgt gatgattcag
     5041 gatgagttaa tgaaggattt tcactaatgc ggatgaatat gtctgacttt gccactttct
     5101 ttgccgtcgc ccgtaatcaa agctttcgtg cagcgggcga tgagttaggc ttatcctcgt
     5161 ccgccattag ccatagtatt aaaacactgg aacaacgtct taaaattcgc ttattcaatc
     5221 ggacaacccg aagcgtatcg ttaacggaag cgggttctaa tctctatgaa cgattgcgcc
     5281 cagctttcga cgaaattcag atcatgttag atgagatgaa tgattttcgt ctgacaccga
     5341 caggaacgct caaaatcaat gccgcccggg tagctgcacg tatattttta atgcctttac
     5401 tggttggttt cacacgtgaa tatcctgata ttaaagtcga gctcacaact gacgattctc
     5461 ttgtcgatat cgtccaacag ggctttgacg ctggcgttcg acttagtggc atcgtcgaaa
     5521 aagatatgat cagcgtggcg atcggcccac cagtgaaatt atgtgtagca gcaacgccag
     5581 agtactttgc aagatatggc aaaccccgcc atcctcatga tctgcttaat catcaatgtg
     5641 ttgtttttcg ttatcccagc ggcaaacctt tccactggca atttgcaaag gagctggaaa
     5701 ttgctgtcgc aggaaatatc attctggatg atgttgatgc tgaactggag gccgttttga
     5761 tgggtgctgg tatcgggtat ctgctctatg aacagatcaa ggagtacctt gatactggtc
     5821 gtttagaatg tgtactggga gactggagca cagagcgtcc aggcttccag atctattatc
     5881 caaaccgcca gtatatgtcc tgcggcttgc gcgcatttct cgattatgta aaaacgggtc
     5941 agatctgcca gagtcagcgt caccgaccac aataattatt tactattcgc tttacgcgcc
     6001 agcagcggga aaatcagtcc taatcctacc agcacaaacg gcgttgcaac attcagcgcc
     6061 aactggaagg tccactctgc agtgaaggct tccattttcg ggaagatccc cgtcaaacag
     6121 gcaaaggcgg taaaggcaaa acaccaaatc ccgacggtca ttgccagagg cttattacga
     6181 ataaagacat attctggttt atatttctgc gccaggcgaa cgactgcaat aaatgcaaca
     6241 aatacccata aataacgcag cggcatcact accgagttaa gattcaacag ccatttatag
     6301 agattgttca tatcaccgat gccgagagtc ggcagcataa tcagaattgc caccagcacc
     6361 agggtcagaa aatagccatt aacgggcgta ccagaagcgt tggtacgaca taaactggct
     6421 ggaatatatt tgctgtcagc atcacctaat agcactttaa gcggggcatc aatcgagaat
     6481 accagcgcag ctacttgtcc cagggtattc gcaatggcgt aaatcaccat taaagtatta
     6541 cccatgttgt aatactcgcc cagcttctga aaggcgtaat attgaccgtt agtcattaag
     6601 tcatccggga tattacgcga gtcaaacatc atccccatcg ccagtgagcc cagaatggca
     6661 caaaccgcaa ccatcaccgc caggcatagc atcccttttg gaaattcttt tcctgggttg
     6721 cgcgtttgat taacgtaagg agaaatcttc tctgctccgc caaccgcgaa aaccagcatt
     6781 gaaatagtgg taatgtaggt aaagtcgata tgaggaatga acgtttccca ggtaatgttt
     6841 gtggtcgcaa tatgcacttc agtaatcgca ggcgcggtta ccgccatcgc cacatacagg
     6901 agcgacataa caaacatcgc aatccctgcc acagaaccga cgattttcag cgatttcata
     6961 ccgcgtgaag caacccacat aaagaagata aacagcacca gcgttaaccc ctgtaacgct
     7021 acgactgaat attctttgat tagcgaaccg tcgcctttca tcgcccaacc gagcgcaatc
     7081 agaattgcct ggggtttttg tgccagatag ggaatatgca ccacccagta ggtccacgcg
     7141 gcgagataag ccagtccggg tcccatcgtg tgtttaatcc aggtactgac cccgcccttc
     7201 ccatctttga aggtcgagcc taactgacca acaattagcg cataaggtgt gaaatagagt
     7261 gcaaagataa acacccatga aaaaacaacc accagcccct ggttggcata gttattaaca
     7321 acgttgccaa aaccccagac agtgataaaa gacatcagcg caatgttgta ccatcgcaac
     7381 tgtttttcct gaacattccc agccatagac aaatccttcg ccaaaattaa aggggcatct
     7441 gtaaaacgag ggattatgcg cggcaaataa gaagtgcgga aatagcgaaa tgcaaaatta
     7501 taacctgctg cacattttat ttttaaacga ataaaatttg gaataaagca agtcagatag
     7561 aagaaaaaaa agcagagtgt gaaacagtaa gttcacactc tgcttgaatt catttacacg
     7621 tgagcaacgg gtttcggcgt atgaccggcg ttgcgcagca gcatcagcaa atagataaac
     7681 gatacgctgg cgatcatgat aaacaataaa ttatcggaga aattctgcat tagcatagcg
     7741 gtaaatgacg ggccaagcag acttcccaca gtatagctca acagtaaggc ctggttcatc
     7801 gccaccagtt gatgatgttc aactttctcg caagcccatg ccatcgccac cggatatagc
     7861 gtaaagccag cggcaccgag gatgaataac gctggggcca tcgccgcctg gctaagcatc
     7921 gcgatactgc cgagaatgac gacaaagacc tgaacacgca acaccagcag tcgaccaaac
     7981 ttatccgcca gacgtccaat cggccattgt ccaaggatac ccgcactgac cagtaccgcc
     8041 atccagaaac caatgctggc attgctcacc cctttgtgat tgaggtacag cggcatcagg
     8101 ccatatagag atcccagaac gattcctgag ataatgcagc cattcacgcc aagccgcgcc
     8161 tgacggagtt ttagcattga cgtaattgac gtcgaatcat ggttttcagc ctgctgattc
     8221 agcacacgcg taaacaacag cggtaagatc cctgccaacg tcaaacctgt aacccacggc
     8281 aatacggaca tcagctcggt tgaaactttg ctgaccagta actggcctaa aaacgttccc
     8341 acgtaataaa ccatcatata cgcagcaagc aaacgcccac ggttacgtga cgtcccactg
     8401 cacatcagcg cgctctcaac aaccacccaa atcatggcac agccgacgcc cgcgacaaaa
     8461 cgccaagcca accagctcca gaatccaatc atcaggccaa ggccggcaca gccagcggca
     8521 aaaatgaagg aggccagata atagctgcgg ttaaagccaa tgcgcttaat gacataccct
     8581 gtcagcaatg taccgacaag gttgccggta aaataggatg agctgacaac gcctacctgc
     8641 catgtggaca tgtgttcctg ggcgagccaa agcggcacga gtgtatttaa caccgcgatc
     8701 gccagagtca acaaaagcag gccagacagc aaaagcatga caggccgggt atacgtggac
     8761 atgaattaaa accgtgagga agttcaaatt tcatgcgcat catgccaccg ccaaaaacat
     8821 tgtcaatggg tgaaaatacg cgcatatcgc agcggcggta ttatcgattt atattactca
     8881 ttcataacgc taatgctgaa gagatgatta atctcatctg attgttttta ttgatctaac
     8941 acaaatttca tacgcttcgt tcagtcgaaa aatttcatgg tcacagcaaa ctcatatttc
     9001 ccgtctatgc tttcagagca atgcatcacg tctgaccgat ttcagacgta ccactgactc
     9061 tcgaaaggag aagaggatga ccaaaccgta tgttcgtctt gataaaaatg atgctgccgt
     9121 tttgcttgtt gatcatcagg ctggtttact ttcccttgta cgggatatcg aacccgataa
     9181 gtttaaaaat aacgtgctgg cgctgggtga cctggctaag tatttcaact tacctactat
     9241 tcttaccacc agttttgaaa ccggtcctaa cggtccatta gttccagaac tgaaagcaca
     9301 attccccgat gcaccttaca ttgctcgccc gggaaatatt aacgcctggg ataacgaaga
     9361 ttttgtaaaa gcagtcaaag cgacaggtaa aaaacagtta attattgccg gtgtggtaac
     9421 cgaagtttgc gtggcattcc cggcgctttc agccattgaa gaagggtttg atgtctttgt
     9481 tgtgactgac gcgtcaggga cctttaatga aattacccgg cattcggcat gggatcgcat
     9541 gtcgcaagca ggtgcgcaat tgatgacatg gttcggcgtg gcgtgcgagc tgcaccgtga
     9601 ctggcgtaat gatattgaag gactggcgac gttgttctct aaccatattc ctgattatcg
     9661 taatttgatg accagttatg acacgttaac gaagcagaaa taaaagagaa ccggggccga
     9721 aaccccggtt gttactgatt agcttgcgac ggccatcccc acggtcatat gcaaaccata
     9781 gaatacgccg cgaccgatta attcccctgc cagaagcaga atgaatgaga cggatagtag
     9841 cggaaccgca ggctgataac cttttagctg tggtgcaatc cacaagcaca gggcaacggc
     9901 caaaagcacg atccgccagg acatcagcgc accatagtcc ggcaccagcg ctgcggcctg
     9961 ctgcacagaa ctatgaatag ttgccagctc tgtgccctgc atcactgaca tcacgccact
    10021 caccaccagt gccagtacag aaatggctgg cagcagacgc atcgcccagc catcgacgcc
    10081 agccagactc aacaacaggt aacccagtaa cgggccgccc ataaacatcg tcaggaagaa
    10141 gcccatcggc gtccagatgc tgtaccaggt cggaacggta tcaatgctgt tatacacacg
    10201 caccatcatc cagacaaaga tgacgccaag aaccatcgtc actatcagcc acagcgtacg
    10261 caatgccggt gacagctttt tcagcattgc cagcaaccag ccgatgccgc ctacggcaaa
    10321 aaagatcgaa ccgctggcga tttcgttact gagtgctgaa gcccctaccc ggttgagcga
    10381 gttaaaagcg cgcattggtg aaccaagatg gagcatagag gcgataaagc caatgcccat
    10441 cagcacccat aaaccaaaca tgcaggcgat aacacgctgc tgggcttctg ctcgcaggtc
    10501 gcctttgagc agcgccaaag ccagaacgat aaaaccacct gctacacatt gcccgaagac
    10561 cgtgaagatc atcagcggcc attcatgcca tccacttccc atctcacacc tccttcgggt
    10621 ttgccagata gccagtggta tccccggtcg ggcggctatt ggcattgggt ttgatcacaa
    10681 tattcggttt ggtaaagtga gctcgcggca acggcgcaac ggccgccaga tcgccatgtt
    10741 ttttacgcag ctcgtcgata gggccgaaat ccagcgcccg cagcggacaa gattcaacac
    10801 agatcggctt tttaccctcc gcaacacggt cataacaacc atcgcatttg gtcatatggc
    10861 ctttcgtttc gttatattgc ggtgcgccat acgggcaagc catatggcag tagcggcagc
    10921 caatgcacac atcttcatcg accacaacaa aaccatcttc acgtttatgc atcgcaccgc
    10981 tcgggcagac tttagtacaa gccggatctt cgcagtggtt acatgaaatc gacagatagt
    11041 aggcaaacac gttctggtgc cagacaccgt tatcttcctg ccagtcgccg ccagcatatt
    11101 cataaatgcg gcggaagctg acttctggcg tcaaatcttt gtagtcttta caggccagct
    11161 cgcaggtttt gcaaccggtg caacggctgg aatcaataaa aaatccatac tgggttgtca
    11221 tcggttactc cttacacctt ttcaacctga acaaggtttg tatgtgacgg attcccctta
    11281 gcgagaggag acggacgttg agtggtcagt acgttgatac aaccgccctt atcgacacgt
    11341 tttgtatccg ggtcatacca ggcaccttca cccagtgcga ccacacccgg catcattcgt
    11401 ggcgtcactt tcgcctcaat atgaacctca ccacgatcgt taaagatcct gactttatcg
    11461 ccgttgttga taccgcgttt ttgcgcatca agcgggttga tccacatttc ctgacggcaa
    11521 gctgctttca gcacatcaac gttgccgtaa gttgagtgaa cgcgagattt atagtggaaa
    11581 cccgtaagct gcagcggata ctgtttgttc agcggatcct gataactttc aaagcccggc
    11641 gtgtagatcg gcagtggatc gatcacatcg ccttctggca attcccaggt agcggcaatg
    11701 tcagccagcg cctgcgaata aatctcaatt ttgcccgatg gcgtagtcag tgggtttgcc
    11761 tgcggatctt cacggaaggc tttataagca acgtgatgcc cttgtgggtc gcgcttttta
    11821 aagatcccct gcttgcggaa ctcttcaaac gttggcagtt caggaatcgc ttcccgcgac
    11881 tgggcataca gatgccgcat ccactcttcc tgggtacggc cttcagtaaa ctgttgctca
    11941 acgccaagac gttttgccag ttcgctggtc atttcataga tggtcttaca ttcaaagcgc
    12001 ggtttaatca cctgatcgtt gaaaatcacg taagacatat tcccgcagga tgcatccagt
    12061 gcaaagtcca tctgttcgga agcggtgcag tcaggcagca ggatgtcagc atatttcgcc
    12121 gatgaggtca tgtggcagtc gataaccaca atcagctcgc acttcttatc atcctgaagg
    12181 atttcatggg tacggttgat ttcagaatgc tggttaatca ggcagttacc ggcatagttc
    12241 cagatcattt tgatcggcac atccagctta tctttcccgc gtacaccatc acgcagcgcc
    12301 gtcatttccg ggccacgttc aatggcatcg gtccacataa acatcgaaat gctggtctgg
    12361 atcgggtttt ccaaggtcgg catacggaca aacggtaagc tgtatgaacc ttcacgcgcg
    12421 ccgctgttgc ctccgttaat accaacgtta ccggtcagaa tcgccagcat cgagatagca
    12481 cgggttgcga tttcaccgtt agcgtgacgc tgcgggcccc atccctggct gataaacgcc
    12541 ggtttggtac tgccgatttc acgagccagt ttgatgattt tgtctgccgg aacaccggtg
    12601 atttgtgagg cccattccgg tgttttagcc acgccatctg gcccttcacc cagaatataa
    12661 gctttatagt ggccattttt cggcgcactg gctggcaggg ttttctcatc gtagccaacg
    12721 caatatttat cgaggaatgc ctgatccacc aggttttcag tgatcatgac gtacgccaga
    12781 ccgttaacca gtgcggcatc tgttcccgga cgaataggga tccactcatc ttcgcgcccg
    12841 gcaccggtgt cggtatagcg cggatcaatg atgatcatgc gggcattaga tttctggcgt
    12901 gcctgttcaa gatagtaagt caccccaccg ccactcattc gcgtttcgcc agggttatta
    12961 ccaaacagca ctaccagctt actgttttcg atatccgacg ggctgttgcc atctgcccag
    13021 ccgccgtagg tatagttcaa accttccgca atttgcgcgg aggagtagtc gccgtaatgg
    13081 ttcagatagc cgccgcagca gttcatcagc cgcgcgacca gggtatttcc cggcggccag
    13141 gagcgggtca tggtgccgcc cagcgtaccg gtgccatagt tcagatagat agactcgttg
    13201 ccgtactctt tgatcaggcg ctgcatattg gtcgcgatga tgtcgtaggc ttcttcccag
    13261 ctaatgcgct cgaatttgcc ttcaccgcgc gccccgactc gtttcatcgg atatttcagg
    13321 cggtccggat tgtagacacg gcgacgcatg gaacgcccac gcaggcaggc gcgaacctgg
    13381 tgcaggccgt cgtaattgtc atcgccggta ttgtccgttt cgacatattt gatttcaccg
    13441 tccacgacgt gcatacgtag cgggcagcga ctaccacagt taactgtaca ggcgctccag
    13501 ataacctttt cgtctgattt tgttggaatg gcgctatcga cagcgtgcgc aatccgacta
    13561 aaaggtaatg ttaatgcgct gctggccatt gccaggccgc cgatcgctgt cgtttttacc
    13621 aaaccacggc gactcacctc agcagccaat accgcatcag ggattttcgt tttcataatg
    13681 gctcactcaa gcttgctcac gattgattcc agtaacaaac cgtattgtgt aattatttct
    13741 atagcagctc gctatatagg cttgtataca tcgaataatt tgaaaatcaa agagataggt
    13801 agattttcac tcgaaagtag tattacgtgt aagtgaggag taattattgt tcggtatcaa
    13861 agggagacaa aaaaagcgcc cgcaggcgct ttttagattc agaaaaattg ggtattagcc
    13921 aatatattcc agtccgttca tatacggacg cagaacttct ggtacttcaa tacgaccatc
    13981 agcctgctga tagttttcca ttactgcaac cagcgtacga ccaacagcca gtccagaacc
    14041 gttcagggta tgaaccagac gggttttctt gtccgacttg ctgcggcagc gtgcctgcat
    14101 acgacgcgcc tggaaatccc aaacgttgga gcaggaagag atctcacggt aggtgttctg
    14161 tgccgggatc catacttcca ggtcgtaagt tttgcaagcg ccaaagccca tgtcgccagt
    14221 gcaaaggatg attttacggt acggcaggcc caacaactgc aggacttttt ctgcatgacc
    14281 agtcatctct tccagcgccg ccattgagtc ttccgggcgc acgatctgca ccatttcaac
    14341 tttgtcgaac tggtgcatac ggatcagacc acgggtgtca cgaccatatg aaccggcttc
    14401 agaacggaag catggggtgt gggcggtcat cttaattggc agatcatctt catcgatgat
    14461 ttcaccgcgc accaggttag tcagcggaac ttctgccgtt gggatcagcg catagttact
    14521 ggtgtctgct tcttcttcca gcggacgagt atgaaacaga tcgccagcaa atttcggcag
    14581 ctgacccgta ccgtacagcg tgtcctggtt aaccaggtac ggaacatagt tctcactgta
    14641 gccatgctgt tcggtatgca gatccagcat aaactgcgac agtgcgcggt gcatgcgagc
    14701 aatctgccct ttcattacca caaagcggga accagtcagc ttaactgcag ctgcaaagtc
    14761 gaggccagag tgcatttcac ccagcgtcac atggtcacga acttcaaagt caaactcacg
    14821 cggggtgccc cagcggctga cttcgacgtt gtcattttcg tctttaccta ccggcacttc
    14881 atctgcaggc aggttaggga tggtcagcgc gatatcgcga atttcagcct gtaaagcatc
    14941 cagctcggct tttgctgcat ccagctcttc gcccagtttg ttcacttcca gacgtaaagg
    15001 ctcgatatct tccccgcgcg ctttcgcctg gccaatggat ttcgatcggg agttacgctc
    15061 cgcttgcagg ttttccgttt tgacctgcaa tactttacga cgctcttcaa gagcgcccag
    15121 cttatctaca tccagcttaa agccccggcg tgccagtttt tcagcgactg cgtctggctc
    15181 attacgcagc agattgggat cgagcatgct tatcctgtgc ttatcgaatt aaatgggaga
    15241 tgtggccaca gcctgcgacc acagggatac agtaacaaca ttaccgcaac gataacatta
    15301 acggtagcgt tttatggggc tattttgatc ctgttcagcc agccaggcga gcttttcgcc
    15361 aatcttgcct tcaaggcccc tgtttgtcgg gaaataatag cgtgtttgtg ctatttccgg
    15421 cgggaagtaa acctcaccgg cagcataagc gtttgcttca tcatgagcgt aacgatattc
    15481 ctgcccgtag cccatttcct tcattaattt cgtcggcgca ttacgcaaat gaaccggcac
    15541 gtcataatcc ggacgttcgc gagcatccgc cagcgcagct ttaaacgcag tgtagacagc
    15601 gttgcttttt ggcgcgcagg ccaggtaaac aatcgcctga gcaatggcgc gttcaccttc
    15661 cgccgggcca acgcgagtaa agcaatccca ggccgcaatt gccacctgca tcgcccgtgg
    15721 gtcggcattg ccgacatctt cggaagcaat tgccaggcaa cgacgcgcga catataacgg
    15781 atcgccacca gcggtaataa ttcgcgcata ccagtagagc gccgcatcgg gtgcgctacc
    15841 acgtaccgac ttatgcagtg cggaaatgag atcgtaaaag cgatcgcctt tgttatcaaa
    15901 gcgggcgctt cgctcaccgg cgatttcggt caataattca ggcttcagga cccgcttacc
    15961 gctatcattg acttcggcca tatccgccat catttccagc gtatttaacg cccggcgcgc
    16021 gtcgccattc accagttcag caatggcgcg tcgtgtttca tctggcagaa caatatcctg
    16081 accaccataa ccacgggtct tgtcttccat cgcctgagtt agtacttgct caatatcctc
    16141 tgtacccagg gatttcaaca gatagacacg ggcacgggaa agcagtgccg aattaagctc
    16201 aaacgacggg ttttcagtgg ttgcgccaat aaaagtgatg gtgccgtctt caatatgtgg
    16261 cagaaatgca tcctgctggc ttttgttgaa tcggtgcact tcgtcaacaa aaagaatagt
    16321 gcggcgacct gcattgcggt tttgccgggc gcgctcgatc gcctcgcgaa tctctttcac
    16381 gccagaggtg acggcagaaa tacgttccac atcagcgttc gcatagcggg caatcacttc
    16441 agcgagggtt gttttgccgg tacccggtgg cccccagagg atcatagaat gtaaatgccc
    16501 ggcttcgata gcgcgcggca acggcttccc cgcagccagc aaatgttgct ggccgatata
    16561 ctgtgctaaa ttttctggcc gcatacgcgc ggccagaggt tgaaaagtat tatccgaaaa
    16621 atcgagcgac agattgctca ctcaggtgcc tctacttacg ttgatcatct accgtgacgc
    16681 cttgcggcgg ggtgaaggta aatttcgctg catccacagc cccattttgc tgggatttca
    16741 gttgataact gctgcgctga tcgtcctgct ccaccgcgct aaactgatgg attgtgccat
    16801 cacgtcccac gttaatggtg aactgcttca gattgccatt gctggctttc ggcgtcagga
    16861 caaagtcatc gccattctgt ttgatattgt actgctgcca gtcgctggac tggttgcggg
    16921 caatcagcat aaacggcgta ttaccggtgg catctttcag ccaggttgcc gtagcttgct
    16981 caacgaacgg gttatagaac cacagtgttt taccgtcaga aaccagaatg ctttcatcag
    17041 gttgtgtcat atgccagttg aataagtttg gacgtttcac ccacagatcg ccctgacctt
    17101 cctgcaccgc cgcgccgcta ccgtcagtca ctttttgtgt gaagctggcg tggaagctgc
    17161 tgactttatc caggcggctt ttcagatcgc ttgcggcatc agcccaaacg ctgcttgcta
    17221 ctaagcttga gagtaatgca caggtgatgg caattttttt catcattatt cctcaaatta
    17281 cgtcactccc gatcgggagt tccctctact ctctattcca ggctaataat cagcttgagg
    17341 aaagaagaaa atactgaatt ttcagttgat agagtgccgg atgcgacgct accgcgcctt
    17401 atccggcaat gcattaatta gtcaaacggc ggtggggcca gcacttcacg attaccgttg
    17461 tgcccctgtt cgctgacaat cccctgcgct tccatctgtt cgataatacg cgctgcacgg
    17521 ttataaccaa tgcggaactg acgctgtacg ccagaaattg acgctttgcg tttttcagtg
    17581 acaaactgca ccgcctgatc gaacaacgga tccagttctt cagcgccatc gaaaccgccc
    17641 gcgccacctt cgctttcact gtcggaagtg atgccatcaa catactgtgg gcgaccacgc
    17701 gctttccagt cctgcaccac ggcatgaact tcctgatcgc gaacaaaagc accatggaca
    17761 cgtaccggca acgtggagtt cggcccagag tagagcatat cccccatccc cagcagtgat
    17821 tccgcgccag cctgatcaag aatggtacgt gagtcaatct tactggatac ggtaaaggcg
    17881 atacgggtcg gaatattcgc tttaatcaga ccagtaataa catcaaccga tggacgctga
    17941 gttgccagta cgaggtggat acccgcggca cgggcttttt gcgccagacg tgctatcagc
    18001 tcttccactt ttttacctac cgtcatcatc aggtcggcaa attcgtcaac caacaccaca
    18061 atgtatggtt cttttttcag caccggatgc tgggcatcca tactgtcacc cggcttccag
    18121 tacgggtctg gaatcggacg catcatgcga tcggcttcag caattttttc gttataaccc
    18181 gccagattac gcacacccag cgcagacatc agtttatacc gacgctccat ctcgttaaca
    18241 caccagcgca gcgcgttggc ggcatctttc atatcagtaa cgacttccgt taacagatgc
    18301 ggaatgcctt cataaaccga aagctccagc attttcgggt cgatcatgat gaaacgcaca
    18361 tcttctggct gtgctttata aagcatgctc aggatcatcg cgttcacacc gacagattta
    18421 ccggaaccgg tagtccccgc aaccaacaag tgcggcattt tcgccagatc ggcaaccacc
    18481 ggctcaccgg cgatatcttt acccagcacc acggttaatg gcgacggatt atcgcggaat
    18541 ttggcgttat ccaaaacttc gcgcagataa acggtttgtc gttttttatt tggtaactcc
    18601 agacctacat agggtttgcc aggaataact tcaacgacac gcaccgccac cgtcgaaagt
    18661 gaacgggcaa ggtcccgtga caagttagaa atgcgcgccg cttttacgcc aggagccagg
    18721 ttaagttcaa agcgagtgat aaccggcccc ggagagtaat tgacgacatc ggctttaata
    18781 cggaaatcag ccagacgcgc ttccaccagg cgagccattt gttcaagcgc aaaggtatct
    18841 accggctcca cttcgctcgg cggcggtgtc agcaaatcca gagaaggcag cggcgtcgtc
    18901 ggtttatgca acggacggct gtcgccatta cgcatcaaca gcggatgaag cagggtatcc
    18961 tgctgctgcg gtgcaaccgg ttgttgcggc tgttgatact gcggctgcgg agcaactggt
    19021 tgttgcggct gctgatattg cggctgtggc gcaaccggtt gttgcggctg ctgatattgt
    19081 ggctgcggcg caacctgctg ttgtggctgc tgatactgct gctgcggcgc aaccggctgt
    19141 tgcggctgct gatattgctg ctgtggcgga actggttgtt gcggctgctg atactgctgc
    19201 tgcggtgcaa ccggttgttg cggctgctgt acaggttcaa caattggcgt aaacaacggt
    19261 tcgtgtggac catcatccag caacgctttc attggcgaaa attcaaaatc atccagcgag
    19321 aacggattcg ccccagccgg ttgttcgccg gaataacgtt gttgttgagt ttgcgcaaac
    19381 tgacgagcca gttcagcctc tgccgcagca tctgcatctt ctgcgtttac gggcacatca
    19441 tgttgatact gttcgccata gcgttgctgc tgtgtctggg cgaactgacg tgccagttca
    19501 tcctgctgca tcgcatcgat ttcatcatcg ttatactgat cgccagaatc atactgattg
    19561 cgctgggctt cacgggcttt ctcttccgcc gcacgctgtg agggcagctt aataccgtaa
    19621 gacgccagtt cacgacgagt tggcacgcgg atacgtttcg gtcgtggcaa ctgcgggcca
    19681 atcccctctt tgacttgcgg acgcggtcca ccgctatttg ccagactgaa gactggcgcg
    19741 gcaacggttg cggcagcccc cgtcgccagt gtcgcttttt tcacgccaga tgccagcgga
    19801 gaaacagcgg cagcggcttc tactggaggt actgctgcaa cagaaggcgc tttcaacgaa
    19861 gatttgatcg gttctggttc tttaaccggt tctggaatcg gttgatacca ggccgcaagt
    19921 tgttcacgtt cacgggctcg cttctcttcc acttcttcaa aatagtaaag cggcggacgc
    19981 gcgggttttg tctcttctac aacgggttca ggctccacaa caggctgctg ttcaacggat
    20041 tgcggctgtt ggtacaacgg ctcctgagcg gctggttgct gataagtttg ctcagtctgg
    20101 tatgtagact gtggagcaaa agtggattgc tgctcttcgg cttgccaggc gttacctgcc
    20161 accggttgtt ctggcgcagg ggcgtaatac ggctgttgtg caggctgttc cggcgcagtt
    20221 gcgtaatacg gttgctgaac aggttgttcc gccgcaggag cgtagtaagg ttgttgcgca
    20281 ggttgttcag ctgcaggtgc ataatacgac tgctgcggct gtactggttg ttgcagcggc
    20341 tcattatatt gcactgcagg ctgcgcatat tgtgactgct gtgggtaacc ttccggtgca
    20401 ggagcaataa ccggctctcc cgtttgtgga cccggtacag gctgccaggc tactgtaggt
    20461 tgcgagggtg gaacatcaac agaggcaaca ggcggcgtct gagtcacagg ttcaaccggc
    20521 gcagcccagc tttgtgtcgc cgtggtagaa gcagctgcta cagcgacagg ttcggtaatt
    20581 ggcgcaccgt ttaataatgg atcgtattcg tcatattctg gctgcgttgc acgattgccc
    20641 gaaaatagga cgtcgtccgg gtcggcagcc acaccgcgtg cagtgtaggt aatctcttcg
    20701 tcatcatcca tccgcttacc ggagaacaac gcagcgtctg tttgccgccc catcggatta
    20761 atgaattttt ccgccaaccg tttacgacgc gctagcgcgc cgcgaagaat acgggcacgg
    20821 cgtgattcat gctgtttgcc gtggttttca tcttcatact cttcgtcatc ttcatattca
    20881 tcttcatcga cccaggtatc atcgcgacgg gtacgattac tggcgaaggt gagaatgttt
    20941 aaaatccagc cgccgagttt ttcagcaatg gtcacccatg accaaccggt gaacagcgtc
    21001 aggcccgcag cccaaacgca gagcagcgca atagttcccc cgctactgtg tagcagtggt
    21061 tgtagcgtag tacttagtaa gctgccaatg acgccaccgg aggcaaaata ccagatatcg
    21121 tcagcgttga ttgccgccag accacaggag gtaaggatga gcgccaaaac gccaatgatg
    21181 cgtagcgaaa cggcaaaata atcaatgtat tcgtcgctgg actgatgacg ccaggcaaac
    21241 caacaaccgc cgacaataat gacgggaatg gtgtaagcca tcacgccaaa aataaagaac
    21301 agcgtatctg ccaaccacgc accgggcatc ccacctaaat tatggatagg ttcatgccag
    21361 gccgtttgcg accagctggg gtccgaaggg ttaaagctta gtaaggcagc catcaaccag
    21421 acggcaaaca ggacaataag gatcagcaac gcctccagaa ggcggcggcc gctacttaac
    21481 tttgtcaatg tgacttcttt gtcttcagtg tattcctggc tcaagaaagg ctctccaggt
    21541 tacaggcttt tcctgcccga tgctaaaacg gacaacagta ccaggttacc ccagtactgt
    21601 tgctgtatgg attaacagga gtgtaatcaa aatacgccga ttttgcacct gttccgtgtt
    21661 agcgcgtctt aataaccaga cgattactct gcttgacttc ttccataaca acgtatgtcc
    21721 gtgtgtcatt gacgccaggc agacgcagca gggtttcccc cagcaactta cggtaagctg
    21781 acatatccgg cacgcgtgtt ttcaacaggt agtcgaaatc accagatact aaatgacact
    21841 cctgaatttc ttcaagtttt tgtacagcgg tattgaattg ttcaaacaca tccggtgcgc
    21901 cacgattcag agtaatctca acgaatacca gaagtgatgc atccagataa tgggggttca
    21961 gcagcgccgt atagccctga ataaaccctt gtctttccag ccgacgcaca cgctcaaggc
    22021 acggcgttgg ggaaagtccc acacgtttag aaagctcgac gttagaaata cgcccatcct
    22081 tttgcaactc attaagaatg ttacgatcga tacggtcgag atctttgcca gggcgcttct
    22141 tgctatctac cattattatt gtctctctgt attccttccc tactcctgtc tgaccctgaa
    22201 acatcactgt tcagagtcgc tacccgtcac gatagaccga aatcatgaag attcacggcg
    22261 cgcaatgcct gggtctacgg tgagaagacc gttgccttgt ggcagctatc gacatcacga
    22321 atggcgtctg tccacgccat acgtagattt cactagcacg gtaaacatgg tatttacatg
    22381 catggttatg cgcatataag aatactgttt ttttcttcct cgaatgtttt cgcaaaacac
    22441 caggggattg tcaaagcaaa acatcgattt ttagtacaac atgccagata ttcattaaca
    22501 ggggcgttta ttcatcattt aatcggctta taaagcggga aatttaagta gcagaggtga
    22561 taatgacggg agaatttgac ctgcttatcg atgctgccga ttgcacaaat tgttaacaaa
    22621 atcgtcatac tcttttttta cgtctgtaaa ttccctacaa tcctgcccat tgtctgccaa
    22681 caactatggg gatctcatgg gcacgaccaa acacagtaaa ctgcttatcc tgggttcagg
    22741 cccggcggga tacaccgctg ctgtctacgc ggcgcgcgcc aacctgcaac ctgtgctgat
    22801 taccggcatg gaaaaaggcg gccaactgac caccaccacg gaagtggaaa actggcctgg
    22861 cgatccaaac gatctgaccg gtccgttatt aatggagcgc atgcacgaac atgccaccaa
    22921 gtttgaaact gagatcattt ttgatcatat caacaaggtg gatctgcaaa accgtccgtt
    22981 ccgtctgaat ggcgataacg gcgaatacac ttgcgacgcg ctgattattg ccaccggagc
    23041 ttctgcacgc tatctcggcc tgccctctga agaagccttt aaaggccgtg gggtttctgc
    23101 ttgtgcaacc tgcgacggtt tcttctatcg caaccagaaa gttgcggtca tcggcggcgg
    23161 caataccgcg gttgaagagg cgctgtatct gtctaacatc gcttcggaag tgcatcttat
    23221 tcaccgccgt gacggtttcc gcgcggaaaa aatccttatt aagcgtctga tggataaagt
    23281 ggagaacggc aacatcattc tgcacaccaa ccgtacgctg gaagaagtga ccggcgatca
    23341 aatgggtgtc actggcgttc gtctgcgcga tacgcaaaac agcgataaca tcgagtcact
    23401 cgacgttgct ggtctgtttg ttgctatcgg tcacagcccg aatactgcca ttttcgaagg
    23461 gcagctggaa ctggaaaacg gctacatcaa agtgcagtcg ggtattcatg gtaatgccac
    23521 ccagaccagc attcctggcg tctttgccgc gggtgacgtg atggatcata tttatcgtca
    23581 ggccattact tcggccggta caggctgcat ggcagcactt gatgcggaac gctacctcga
    23641 tggtttagct gacgcaaaat aattttacaa atcagtaaca aaagtaaaga aggcgacacc
    23701 atgcgactat gggtcgcctt tattttttcc ccgttgtaac attgctctgc aaataattct
    23761 gataactcac ctgctaagcg tgcaatgaat aaatcccgtc aaaaagagtt aacccgctgg
    23821 ttaaaacagc aaagcgtcat ctcccaacgt tggctgaata tttctcgtct gctgggcttt
    23881 gtaagcggca tattgatcat tgcccaggcc tggttcatgg cgcgaattct gcaacatatg
    23941 attatggaga atattccccg tgaagccctg ctgcttccct ttacgttact ggttctgacc
    24001 tttgtactgc gcgcatgggt ggtctggtta cgcgaacggg tgggttatca cgccgggcag
    24061 catatccgct ttgccatccg ccgtcaggtt ctcgaccgtc tgcaacaagc agggccagcg
    24121 tggattcagg gtaaacctgc ggggagctgg gcgacgttgg tactcgagca aattgacgat
    24181 atgcatgatt actatgcacg ctacctgccg caaatggcgc tggcagtgtc ggtgccgttg
    24241 ctgattgtgg tggcgatctt cccctctaac tgggctgcgg cgctcattct gctgggcaca
    24301 gcaccgttaa ttccgttgtt tatggcgctg gttgggatgg gggctgccga tgctaaccgg
    24361 cgtaactttc tcgctcttgc tcgcttaagt gggcatttcc tcgatcgcct gcgcggcatg
    24421 gaaacattgc gtatttttgg tcgtggtgaa gctgaaattg aaagtattcg ttctgcttcg
    24481 gaagatttcc gccaacggac aatggaagtg ctacggctgg cgtttttatc atccggcatt
    24541 ctcgaatttt ttacctcgct gtcaattgct ctggtggcgg tctactttgg tttttcctat
    24601 ctcggcgagc tggattttgg tcactacgat accggtgtga cgctggctgc gggttttctg
    24661 gccctgatcc ttgcgccaga gtttttccag ccattgcgcg atctcggtac gttttatcat
    24721 gctaaagccc aggctgttgg tgcagctgac agtctgaaaa cgtttatgga aaccccgctc
    24781 gcccatccgc agcgtggcga ggtggaatta gcatcgaccg atccggtgac cattgaggcc
    24841 gaggatctgt ttatcacgtc gccggaaggt aaaacgctgg ccggaccgct gaactttact
    24901 ttgccagcag gccaacgtgc agtgttggtt ggtcgcagcg gttcaggtaa aagctcactg
    24961 ctgaacgcgc tttctggttt tctctcatat cagggatcat tacgaatcaa cgggatagaa
    25021 ttacgcgatt tatcaccaga atcatggcgt aaacatctct cctgggttgg gcaaaaccca
    25081 caattaccgg cagcaacatt gcgggataac gtactactgg cgcgacctga tgccagcgaa
    25141 caagaattac aagcagcgct ggataacgcc tgggtcagcg agtttctacc gctcctgcca
    25201 caaggcgttg atacacctgt tggcgaccag gctgcccgcc tttccgtggg gcaggcgcag
    25261 cgcgtggcgg tggcccgtgc gttactaaat ccctgttcgc tattactgtt ggatgaaccc
    25321 gctgccagcc ttgatgctca cagtgaacag cgcgtaatgg aggcgctgaa tgccgcctct
    25381 ctgcgccaga caacgttaat ggtcacccac cagttagaag atcttgctga ctgggatgtc
    25441 atttgggtta tgcaggatgg ccggattatt gagcaaggac gttacgcgga attaagtgtg
    25501 gctggtggcc cattcgccac attactggcc catcgtcagg aggagattta aatgcgcgct
    25561 ttgctaccct atctggcact gtataaacgt cataaatgga tgttaagtct tggtatcgtg
    25621 ctggcaattg tcacgctgct cgccagtatc ggtctgttga cactttccgg ctggttcctc
    25681 tcggcctcag cggttgcggg ggttgccgga ctgtacagct tcaactatat gctacccgct
    25741 gcgggcgtgc gtggcgcagc aatcacccgt actgccgggc gttattttga acgtctggta
    25801 agtcacgacg cgactttccg cgtgttgcag catctgcgca tttacacctt cagcaaattg
    25861 ctgcccctct cccctgccgg actggcgcgc tatcgtcagg gcgaattgct caatcgcgtg
    25921 gtggcggatg ttgatacgct cgatcatctt tatctgcgcg ttatctcgcc gctggtgggc
    25981 gcttttgtgg tgattatggt ggtgacaatc gggttaagtt tccttgattt caccctcgcc
    26041 tttacgctgg gcggcattat gttactgacg cttttcctga tgccaccgct gttttatcgt
    26101 gcgggaaaaa gcaccgggca aaatctgact catcttcgcg gacagtatcg ccaacaactg
    26161 acggcctggc tgcaagggca agctgagctg accatttttg gcgccagcga tcgttatcgc
    26221 acgcaactag agaatacaga aattcaatgg ctggaagcgc aacgccgtca atctgaactg
    26281 accgcattgt cgcaagcgat aatgctgctc attggcgcgt tagcggtgat cctgatgctg
    26341 tggatggcgt ctggcggcgt tggcggcaat gctcaacccg gcgcgttaat tgccctgttt
    26401 gtcttctgcg cgttagccgc gtttgaagca ctggcaccag taacgggtgc atttcagcat
    26461 ctggggcaag tcattgcctc tgccgttcgg atcactgatt taacggatca aaaaccggaa
    26521 gtcaccttcc ctgataccca aactcgtgtt gccgatcgcg tttcgctgac attacgggat
    26581 gttcagttca cttatccgga gcaatcccaa caggcactta aagggatttc tcttcaggta
    26641 aacgccgggg aacatattgc gattctcggg cgaaccggat gcggcaaatc aacactgtta
    26701 caactgctga cccgcgcttg ggacccgcaa cagggagaga ttttgcttaa cgatagcccc
    26761 atagccagcc tgaatgaagc ggctctacga cagactatca gcgttgttcc tcagcgagtg
    26821 catctgttta gcgccacgct gcgtgataat ctgttactcg cctcgcctgg cagtagtgat
    26881 gaggctctgg cggagagctt gcgtcgcgtt ggtctggaga agctgctcga ggatgcaggt
    26941 ctcaacagtt ggttaggtga aggcggacgc cagctctccg gtggtgaact gcgccgtctg
    27001 gctatcgccc gtgcgctgtt acatgatgcg ccactggtgt tgctggatga acctaccgaa
    27061 ggcttagatg ccacaaccga aagccagatc cttgaattgc ttgcagaaat gatgcgtgag
    27121 aaaacggtgt taatggtcac ccatcgactt cgcggactct ctcgtttcca acaaataata
    27181 gtgatggaca acgggcaaat tattgagcaa ggtactcacg cagagctgct tgccagacag
    27241 gggcgttatt accagttcaa gcagggtttg taagctatta ttgaacgatc cgacttgcgt
    27301 ggagttttgc ggtcatgcgc ctggttcagc tttctcgcca ttcaatagcc ttcccttccc
    27361 cggaaggcgc attacgtgag cctaacggcc tgctggcact tgggggcgat cttagccctg
    27421 cgcgcctgtt aatggcttac cagcgtggta tttttccgtg gttttctcca ggcgacccca
    27481 tcctctggtg gtcgcccgat ccccgcgcgg tgctatggcc agaatcactg catatcagcc
    27541 gtagtatgaa gcgatttcat aaacgctcgc cctatcgtgt cacgatgaat tacgcttttg
    27601 gtcaggtcat tgaaggctgt gccagcgatc gcgaagaagg aacctggatc acgcgtggcg
    27661 tggtcgaagc ctaccatcgc cttcacgaac tcggccatgc ccactccatt gaagtctggc
    27721 gtgaagatga gcttgtcggc ggtatgtacg gcgtggccca gggaacgcta ttttgtggcg
    27781 agtccatgtt cagccggatg gaaaatgcgt ctaaaacggc gcttctggta ttctgtgagg
    27841 aatttatcgg tcatggcggt aagcttatcg actgccaggt ccttaacgat cacacagcat
    27901 cgcttggtgc ctgcgaaatt ccccgccggg attaccttaa ttatctcaat caaatgcgcc
    27961 tcggacgatt gccgaataat ttctgggtac cacgatgctt gttttcacca caagaatgaa
    28021 tgttttcggc acatttctcc ccagagtgtt ataattgcgg tcgcagagtt ggttacgctc
    28081 attaccccgc tgccgataag gattttttcg cgtcaggtaa cgcccatcgt ttatctcacc
    28141 gctcccttat acattgcgct tttggtgcgg cttagccgtg tgttttcgga gtaatgtgcc
    28201 gaacctgttt gttgcgattt agcgcgcaaa tctttactta tttacagaac ttcggcatta
    28261 tcttgccggt tcaaattacg gtagtgatac cccagaggat tagatggcca aagaagacaa
    28321 tattgaaatg caaggtaccg ttcttgaaac gttgcctaat accatgttcc gcgtagagtt
    28381 agaaaacggt cacgtggtta ctgcacacat ctccggtaaa atgcgcaaaa actacatccg
    28441 catcctgacg ggcgacaaag tgactgttga actgaccccg tacgacctga gcaaaggccg
    28501 cattgtcttc cgtagtcgct gattgtttta ccgcctgatg ggcgaagaga aagaacgagt
    28561 aaaaggtcgg tttaaccggc ctttttattt tgtgatatgt atgaagtact ttggaagtat
    28621 aagtccgtaa cttgtctcga tgtagatata ctgcacaact tgtttacagt atgagcaaac
    28681 aataattatc attaaaattc ttcttgaccg ttttactcca actccctata gtagcgcccc
    28741 gttgccaccc atgaggtttg gtagcaaaac aatttggtga ggtgtccgag tggctgaagg
    28801 agcacgcctg gaaagtgtgt atacggcaac gtatcggggg ttcgaatccc cccctcaccg
    28861 ccatatttaa agaagagctc gtacgaaagt gcgagctttt ttttcgcatt ttatctgtct
    28921 gtacggcagt gaacgcgttc gcaaaggttt taccgcaccc cgtcgatttc taagctgcct
    28981 gtacggcagt gaactcaaat actacacgtt tagcccgttg ttaataaagc aaaaccatcc
    29041 cattctcctg cagcaccctt ttttatcctt accttcccgc agcactaatt atcaacaact
    29101 taccatcatc attaaaaaaa gggtctgaac ctcctccacc ccataaacaa aaagtccgta
    29161 acctctttcg agattacgga cttgaccaac ctacctaaca atcagattaa tgcgctgctt
    29221 ccgccttgtg cttttgtgca ctctggaatc cgtaagtcag ctcatttttc tctttatcca
    29281 gcgcgacggt gacctgaccg ccgtccacca gcgaaccaaa cagcagttcg ttggcgagcg
    29341 gttttttcag gttgtcctgg atgacacgcg ccatcggacg tgcgcccatt gcccggtcgt
    29401 aacctttctc ggccagccag ttacgcgctt cctggctcac ttccagagaa acacctttct
    29461 gatccagctg aacctgcaac tcgacgatga atttatccac cacctgatgg atcacgtcgg
    29521 ttgacagatg atcaaaccag ataatgttgt cgagacggtt acggaattcc ggtgtaaaga
    29581 tcttcttgat ctcctccatc gcatcggtgc tgttatcctg gtggataaga ccaatggatt
    29641 tacgctcagt ttcacgtacc ccggcgttgg tggtcatcac cagcaccacg ttacggaagt
    29701 ccgctttgcg tccgttgtta tcggtcagcg taccgttgtc catcacctgc aacagaatat
    29761 tgaacacgtc cggatgcgct ttctcgattt cgtccagcaa cagcaccgca tgtggatgct
    29821 tgatgaccgc atcagtcagc aaacctccct gatcaaaacc aacgtatccc ggaggcgcac
    29881 caatcagacg gctgacggta tggcgttcca tatactcgga catatcaaag cgcagcagct
    29941 caatgcccaa cgctttcgaa agctgtaccg tcacctctgt tttcccgacc ccggtagggc
    30001 cggcaaacag gaacgaacca accggtttat gttcgtgacc taaacctgca cgcgccatct
    30061 taatggcttc agtcagcgcc tcaatggctt tatcctgacc gaagaccagc attttcaggc
    30121 gatcgccgag gtttttcagg gtatcgcggt cactctgaga aacactcttc tctggaatgc
    30181 gcgcaatacg ggccaccacg gactcaatat ccgccacatt aacggttttc ttgcgtttgc
    30241 ttaccggcat caggcgtgcg cgagcgcccg cttcgtcgat aacgtcaatg gctttatccg
    30301 gcagatgacg atcgttaatg tatttcaccg ccagctctac cgctgcacgc accgcttttg
    30361 cggtataacg cacgtcgtgg tgcgcttcat acttcggttt caggccattg atgatttgaa
    30421 cagtttcttc gatcgacggt tcagtaatat caattttctg gaagcgacgc gccagagcac
    30481 ggtctttctc gaaaatgttg ctgaactcct gataggttgt cgaaccaatt acgcgaattt
    30541 taccgctgga gagcaacggt ttgatcaggt tagccgcatc gacctggcca ccagacgctg
    30601 cacccgcacc gataatggtg tggatctcat caataaacag gatgctgtta gtgtcctgct
    30661 ccagctgctt gagcaacgct ttaaaacgtt tttcaaagtc gccgcgatat ttagtgcccg
    30721 ctaacagaga accgatatcg agagagtaaa tcgtacagtc agccatcact tccggcacat
    30781 cgccctgaac aattcgccag gcaagacctt ccgcaatcgc ggttttaccg acaccagatt
    30841 cccccaccag cagcgggttg tttttacggc gacggcagag aacctgaata gcacgctcca
    30901 gctccttctc acgaccaatc agtgggtcga ttccgcccac gcgcgcaagc tgattcaggt
    30961 tcgtcgtgaa attctccata cgttcctccc caccagcttg ttcttcgctg tttggctggc
    31021 tgccaggatc agaagactgt gtcggctcgt ctttacgcgt gccatgagag ataaagttca
    31081 ccacatcgag acggctgact tcatgtttac gcaacagata tgccgcctgc gactcctgtt
    31141 cgctaaagat agcgaccaga acgtttgcac cggttacctc attgcgaccg gaggactgga
    31201 catggaagac cgcacgttgc agtacacgct gaaaactcag cgtcggctgt gtgtcgcgct
    31261 cctcttcact ggcaggcaga acgggtgtgg tttgttcaat aaaggcttcc agttcctgac
    31321 ggagcgcaac caaatccaca gaacacgctt ccagcgcctc ccgggcagat gggttactga
    31381 gcagcgccag taacaagtgc tcgacggtca taaactcatg acggtgctcg cgcgctctgg
    31441 cgaaagccat atttaaactg agttccagtt cttgattgag cataggcacc tcccccaatt
    31501 tttatgcctg cattcaggct ttttctagcg tacacagcaa tggatgctca ttctccctcg
    31561 cgtacttgtt caccatcgcc actttggttt ctgcaacctc ggcggtaaag actccgcaaa
    31621 tggccttccc ctggtagtga acagcgagca tcaattgcgt tgcacgttct acatcataag
    31681 aaaagaattt ttgtaacacg tcaataacaa actccatcgg agtgtaatca tcattgacta
    31741 atatcacttt atacatagat ggcggtttta gcgcgtcgcg aactttttct tccgccagtt
    31801 gatcaaagtc cagccagtcg ttcgttttac ccattgtcag ttatcatctt cggttacggt
    31861 tatcggcaga acgccctgcc gctgacaaga gcttatttgc ctgaccaatc tactccacat
    31921 gatagataac tatcatctat cgttggcatc agcgacatct gtcacattcc tgtcaatagc
    31981 gttaactgct tcaaattttt gattcatacc tgccccctgg cccctgtcaa acgcttgacg
    32041 gctcgcccta attctctaaa ttgtatttct agagttggcg aggttttgaa cagccccctc
    32101 tctgaccccg gtttattcca tcttacttgt ataagatttg cgaaggatgt cgaagcatgg
    32161 aaaagggtac tgttaagtgg ttcaacaatg ccaaaggatt tggtttcatc tgccctgaag
    32221 gcggcggcga agatattttc gctcattatt ccaccattca gatggatggt tacagaacgc
    32281 taaaagctgg acaatccgtt cagtttgatg tccaccaggg gccaaaaggc aatcacgcca
    32341 gtgttattgt gcccgtcgaa gtagaagcgg cagtcgcata gctcttctgt ctcattgtgt
    32401 acatcctaaa ggcaaaatgc cagcccgatc ggctggcatt tttatctcaa aaattactct
    32461 cgtgccagag catctactgg atccagtcgt gccgcatttc gtgcgggtaa ccatccaaat
    32521 aaaatcccgg tgaccgtcga gcagagaaac gccagcagca gtgccaacgg tgaaaaacca
    32581 atctcccagc cgggtaagaa aagctgcaag gtgaaagcaa ttaacagtga cagtgttatt
    32641 cccaacgcgc caccgaccag gcaaaccagt acggcttcga tcaggaactg ttgcaaaaca
    32701 tcgcttgctc gcgcacctac agccatgcga atgccaattt cccgcgtccg ctcggtcact
    32761 gacaccagca taatattcat tacaccaata ccgcccacca ccagcgaaat caccgccacc
    32821 agcgtcagaa acagttgtaa agtacgtgtg gtcttttcaa cagttttcaa gacgccgtcc
    32881 atgttccagg taaagaaatc cttctttccg tggcgcagtg aaagtaaacg cgtgagttgc
    32941 tgttccgcct cggcgctgtc aaatccttct tttaccctga cagtaatgga gttcagccac
    33001 gactggccca taactcgccc ggacatcgtg ctgtaaggta gccagacacg cagcacttta
    33061 ctgctaccaa acatcgactg tttttcttcc gccacaccaa tgactctggc gggcatattg
    33121 ccgaccagaa tcacctcgcc aaccacatct gctttatggg ggaaaagctg gcggcgagta
    33181 ttactgtcga gaaccacgac ctgcgcacga ccgttcagct gctcctgatt aaaggtgttt
    33241 ccttcactga aggtcatgcc atagacatta aaataatcgc cgctcacgcc attggcactg
    33301 gcagcaacat caacattgtt ataacgcagg cgcaggtttt gcgagacggc aggtgtggct
    33361 gaggcgaccc acggttgttt ttggatggcg attaagtcgt cgtacttcag cgcctgctga
    33421 tattgcggat cgtcatcgcc aaaatctttc ccgggataga catcaatagt attcgtacca
    33481 atagaacgaa tatccgccag caccatttgt ttggcggcgt cacccaccac gacaatggaa
    33541 accaccgacg caataccgat aataatcccc agcatggtca gtaaagtacg cattttattc
    33601 gctgccagcg cccgccatgc catcgtcagc gcctcgttaa aaccgctgac aaactgccgc
    33661 cagccagaca ccgtgttgac aacaggctcc gtcccgccag caacattcac tttttcaatg
    33721 gcgggaggat tgcgcacaat ttcgccgtcg cgaatttcga tcacccgctc ggcctgagca
    33781 gcgacctgcg gatcgtgggt gacgataatc accgtatgcc cacgatcgcg cagctgatgc
    33841 aggatcgcca tcacctcttc gccagaatgg ctgtccagtg cgccggttgg ttcatcggca
    33901 agaattacct gaccaccgtt catcaatgcc cgcgcgatgc tgacgcgctg ttgctgacca
    33961 cccgaaagct gtgccggata atactctgta cggtcttcca gccccagccg ttgcagcaac
    34021 tcctgggcac gaagcagtcg ctgtttccgc tcaagaccag catagacggc gggtacttca
    34081 acgttctgct cggcggttaa atgcgaaagt aaatggtaac gctggaaaat aaagccgaaa
    34141 tgctcgcggc gcagttgcgc cagcgcatcg gcgtccagtg tggcaacatc ctgaccggcg
    34201 acgcgatagg tgccgctggt ggccttatcc agacagccga gaatattcat cagggtcgat
    34261 ttaccggaac ctgaagcgcc aacaatcgct accatctcac cggcataaat atcgagggtg
    34321 atgcccttca gcacctcaac ctgctcatca ccggcaggat agctgcgacg aatatccttt
    34381 aattcgagca aaggcgtcat tgtgcagctc ctggtttggc ctcaccaatc accacttcat
    34441 cgcccgcttc aagccctttg acaatctcaa catcggtatc gttacgtgcg ccaatcgtca
    34501 cttcacgctc gcgtgtttca ccattacgca acaatttgac tttataacga ttatcgccaa
    34561 ccggatcgcc taacgccgac agagggatcg tcagcacatt tttcacatcg gtgagctgaa
    34621 tatgcacttg cgcagtcata tccagccgca gcaaaccatt ggggttgggg acttcaaaac
    34681 gggcgtaata gaaaatagcg tcgttaacct tttccggcgt cggtagtaca tccttgattt
    34741 gcccctcgta gcgcgtcagt ggatcgccaa gtaccgtaaa ccaggctttt tgccccggct
    34801 tcaggtggat tacatccgct tcagaaacct gcgcttttac cagcatggtg ctcatatctg
    34861 ccagcgtcag aatgttcggt gcttgttgtg cggcaatcac cgtctggcct tgcagagtgg
    34921 tgatttgcgt gacttccccg gccatcgggg caacgatgcg agtgtaatcg agattggttt
    34981 tagccgtatc gagagaggcc tgattgcgct tgatttgcgc gtcaatggtg ccaatttgcg
    35041 cctgtttcac agccatctcc gtcgcggcgg tgtcgagatc ctgctgtgaa accgcctgcg
    35101 tttgtgccag acgttgctga cgggaatacg tcacccgcgc cagtttcagc tccgcttccg
    35161 cctgctgccg ctgcgcacgt agctccatca gcgttgcttc gacctcctta atctggtttt
    35221 cagcctgttc aggatcaata acccctaaaa gctggtcttt ttttacttta tcgccaatcg
    35281 ccaccgacag agttttcaac tgaccgctga cctgcgcgcc cacgtcaacc ttacgcagcg
    35341 cgtccagctt tccggtcgcc agcacgcttt gctgtaaatc accggggcgc acaatcagtg
    35401 tctgataagt cggcacgggt gcgttaagaa ttctccataa cgtaatcagt ccggcgatga
    35461 ctatcaccag cgcaataacg taacgcttct tcacggtttt ccgctttttc ataaatattc
    35521 tgaatactcc atatgccaat ccgggtcatt aacttcaacg aaatatcaac aaaaccctaa
    35581 aatttacgtt agtaatcaca ctattgatgt ttggttaaga tacgcaatgg tgaaatcgac
    35641 gtcatgtata accattgatt tcatgaatat gtcgcagcta actgaacgga cctttacgcc
    35701 atctgaatct ctcagcagcc tgtcactttt tcttagtctg gcacgtggac agtgtcggcc
    35761 gggtaaattc tggcatcgcc gtagttttcg ccagaaattt ttgctgcgct cgttgattat
    35821 gccgcgttta agcgttgagt ggatgaacga actttcccac tggcctaatc tcaatgtgtt
    35881 gttaacgcgc caaccgcgac tgcctgtgcg tctgcatcgc ccttaccttg cggcgaatct
    35941 tagccgtaag caattgctgg aggcgttacg ttaccattat gcgttactcc gcggatgtat
    36001 gtcggcggaa gaattcagct tatatttgaa tacccccggg ctgcaactgg ctaaactgga
    36061 aggcaaaaac ggcgagcagt tcacgcttga gctgaccatg atgatctcaa tggataaaga
    36121 aggtgacagc acaatcctgt tccgcaacag cgaaggtatt cctctggcag agctcacttt
    36181 taccctgtgt gaatatcagg ggaaaagaac gatgtttatt ggcggactgc aaggcgcaaa
    36241 atgggaaatc ccacatcagg aaatccagaa tgcgacgaaa gcctgccacg ggctatttcc
    36301 caaacgcctc gtgatggaag cggcctgtct gtttgcccaa cgtttgcagg tagagcagat
    36361 tattgccgtc agcaatgaaa cgcatattta ccgcagcctg cgttatcgtg ataaagaagg
    36421 caagatccat gccgattaca acgctttctg ggaatcggtt ggcggcgtat gtgatgctga
    36481 acgccattac cgccttccag cacagatagc acgaaaagag attgccgaaa tcgccagtaa
    36541 aaaacgggct gaataccgtc ggcgctatga gatgctcgac gctattcagc cacaaatggc
    36601 cacgatgttt cgcggttaat ccgcgcgacc gcgcgccagc cacagcacgc gtgagaacat
    36661 ttttttcagc agcgtcggta cggagtccac accacgacgt ccggcctcca ttgccacttc
    36721 aatggcaaga tcgggtttgg aagagcgatg gatcgctttc gagataattt tgcgcagatt
    36781 catcggtaca ttttccggga tttgcgccac gcggtggaac acatcggaaa atccctggcg
    36841 atacataaaa tgttccatat ccagcgccgg taacgccgtt aaatgttctc gttcggcttc
    36901 ccgatcgtta ttcaacaggc tgcgtaccgt agcggcatat ttcttccctg cttcatcgcc
    36961 atcgaccagt acatgccatt caatccccat tcggcgggca aatttaacca gtggctttag
    37021 cccggactgg gcaaactcaa tgaccttgat cccttcggca tcgaaatggt gtccacactg
    37081 gcgcgccagt tcattgataa cccaggtttc cgtttcccct tccaccagca accagcagcg
    37141 agcaaacagc gatgacggac ggttaaaacg aatgtgaaag gatatgcgtc ggctatcttc
    37201 ggtactcaag ccactcggcc ccagacgcca ggcggcaacg cgcgaggact cacgtaccag
    37261 tcggcaaaca tgctctaccg gcgttaacga aagcaactca cccgagttgg tggtggcaat
    37321 gcgctgcaat ggcagaagat tcaacagatg ccaggcaact gaaagcataa tggggtgtaa
    37381 acgggtttct ggatcttcaa ttagcaataa cggacgcgcg tctttatcca gtctcaatgt
    37441 gccttttgcc tgcaataaag tagcaaacag gccgagcaaa ataacccgat acgagcgccc
    37501 tccaggtcgg tcaatcatcc ggttgatgat gtccagatag cgccagcttc gttgctcatt
    37561 gctggcccgc cgccgcatta aacgatatcg cgcctgtcca gccccctgct cagagaaata
    37621 atgctcaagc aactgcacca ttgcggaaag tccctgacga atctgtccat cagagagatt
    37681 ttgcggatgt gaggacaact cacgggcgag gaaatcgagc tggcgcgcgg tgacttccac
    37741 attgggaaca tttggcaccg tgccgttacg aatacggcgc ataaaacggg catcacgcaa
    37801 gcgcagcacc ggcattaaac gcaccagatg gcgtgcctga tcgttaatat cctcgacatc
    37861 aatcggatgt ccgtctttat cgagaaaact gcgcagtgtc atcacgctgc cgtcttccgc
    37921 actctcccct tccagacgat aaaaaatacg gtgatagcca tcggtgcatg gcgtccagca
    37981 cgcttccagc ggccgataac ggcgaacccg atgtcggcct ggcagcgatt cgcggaaggt
    38041 caaaataata tgcagatgat gttctcgccc gttgatatct cccggcggga accagaaatc
    38101 gtcgcgctca aaatggtaga gatctgattc tggcgatagc agcagggtta aggcgtccag
    38161 caagctggat ttaccccacg cgttctcccc aatcaggacg ttgttttgtt cgagcatcaa
    38221 cgacaaacgg ttgataccgc gaaaacccac aatttcaacg cgctcaagaa tcatatcccc
    38281 tccagaaata tgtgacttat cctttacgct ggcagtataa cgggaaaggc cttgctaata
    38341 cgtgatgaca tcacatttaa gcatggcaaa tgtctgaaaa atgagcgaat aatcgcgtta
    38401 tactccctct tgctgataag tctgcaaaaa caacaaaatc aaataattag catgataaat
    38461 tattttttat tacgcctaaa ggattaaatc ttttgcttgc actacactgt caatcagaca
    38521 aatgactata tggggcaacc cgctgttgcc ctaaatcaaa tttactggat ttatttatct
    38581 ggtgaatgac tcgccttcgt ttttaaaata gccgcgcatt atttccgtcg tcactttttt
    38641 acgacgaccc tataaaacga ccatattttt cacagggtca atttttaatt gaggtggata
    38701 tgttcagaaa attagcagct gaatgttttg gtactttctg gcttgttttt ggtggctgtg
    38761 gtagtgctgt actggccgca ggcttcccgg aattaggcat tggttttgcc ggcgtggcgt
    38821 tggcgttcgg tctgaccgtt ctgacgatgg cctttgctgt tggtcatatt tctggtggtc
    38881 attttaaccc ggcggtcact attggtttat gggctggcgg acgttttccg gcaaaagaag
    38941 tcgttggcta cgtaattgcc caggttgtcg gcggtattgt tgcagcggcg ctgctgtatt
    39001 taattgccag tggtaaaacg ggttttgacg cggcagccag cggttttgct tctaacggtt
    39061 atggcgagca ttcaccaggc ggttattcca tgctttccgc gctggtagtt gaactggtat
    39121 tgagtgcagg tttcctgttg gtgatccacg gcgcaaccga caaattcgcg ccggcaggtt
    39181 ttgcgccgat cgctattggt ctggccttaa ccctgattca cttaattagt attccggtga
    39241 ctaacacttc tgttaacccg gcgcgcagca ccgcggttgc tatcttccag ggcggctggg
    39301 cattagaaca actgtggttc ttctgggtgg tgccaattgt cggcggcatt atcggtggtc
    39361 tgatttaccg gaccctgctg gaaaagcgtg attaattaac gcagaaggcc tggcgtaatg
    39421 tcaggccttt ttttaagatg ttgttatatg ttgattcatc atcttgttgc gctcctttca
    39481 tgagctaagc cacatattgc cactggcgca aggagcgcgc agggggcggc caatcgccgc
    39541 cgccccatgc acccccgggc tctggcgaac aaaatcgccg ctgcgcggtg ccctcgactt
    39601 atcccttacg gctaccgggt cgggcgcgag gtaacatccc tgtaaaacgc gccctcagcc
    39661 cacatccatg tgggctgccc cggccttcag ggaacgcctc agcgattttg acgccaccaa
    39721 catcggtgca gtttgtaatt aaagcgcaag aatatcttta aatgaaaacg aatactctac
    39781 tgctccttat gcataacatt tccttttaat tgcaacttta ctcgtccttc cgctttcagt
    39841 agtgtgtcat cgcgcatttc gacttcatct ttgcaaggac tgggttttca tgttttctgg
    39901 gctgttaatc attctggttc ccctgattgt gggttacctc attccgcttc gccaacaagc
    39961 tgcgttaaaa gttattaatc agctattaag ctggatggtt taccttattc tcttttttat
    40021 gggtataagt ctggcgttcc tcgataacct cgccagtaac ctgttggcga ttctgcatta
    40081 ttctgccgtc agtattaccg ttattttact gtgtaatatt gccgccctga tgtggctgga
    40141 gcgaggcctg ccgtggcgca accaccatca gcaagaaaaa ctcccgtcgc gtattgcgat
    40201 ggcgctggag tcgctaaaac tgtgcggcgt agtagtgatt ggttttgcca ttggtctgag
    40261 tggactggct ttcttacaac acgcgaccga agccagtgaa tacacgttaa ttttgctact
    40321 tttcctcgtt ggtattcagt tgcgcaataa tggcatgacc ttaaagcaga ttgtccttaa
    40381 tcgccgggga atgattgtcg ccgtggtggt ggttgtcagt tcattaattg gtggtttaat
    40441 taacgccttt attcttgatc tccccatcaa taccgcgctg gcaatggcct ccggtttcgg
    40501 ctggtattct ctttccggta ttttattgac cgaatctttt ggtccggtaa tcgggagcgc
    40561 ggcgtttttt aatgatctgg cccgtgaact gattgctatt atgttgatcc ctgggctgat
    40621 tcgccgcagc cgctctactg cactgggctt atgcggtgcc acatcaatgg atttcaccct
    40681 gcccgttctt caacgtactg gcgggctgga tatggtcccg gcggcaattg ttcacggttt
    40741 tattcttagc ctgttagtgc cgatcctcat cgcctttttc tctgcgtaat acctctctgg
    40801 cggtagattc ctgccgccaa aattgcgcta aatcaatctc ccttaaagtt gcatgaaaaa
    40861 tcccttttat ccccgcgtta agcgtcttaa ccttaaacat gtatattaaa tataacttta
    40921 aaaggtgtga tcatgttttg tgtgcaatgt gaacaaacta tccgtactcc ggcaggaaac
    40981 ggctgctcat acgcgcaggg gatgtgtggt aaaacggcgg aaacttctga ccttcaggat
    41041 ttactcatcg cggcgctgca agggctttct gcctgggcgg taaaagcgcg tgaatacggc
    41101 atcatcaacc acgatgttga cagcttcgcg ccacgtgcct ttttctcaac tctgaccaac
    41161 gttaacttcg attctccgcg tattgtcggc tacgctcgtg aagcgattgc cctgcgcgag
    41221 gcgctgaaag cgcaatgcct ggctgtagat gccaacgcgc gcgtcgataa cccgatggct
    41281 gacctgcaac tggtgagcga cgatctcggc gagctgcaac gtcaggcagc agaatttacc
    41341 cctaacaaag ataaagcggc gattggcgaa aacattctcg gcctgcgtct gctgtgcctg
    41401 tatggcctga aaggtgcggc ggcctatatg gaacacgcgc atgttctcgg tcaatacgac
    41461 aacgatattt atgcccagta tcataaaatc atggcgtggc tggggacctg gcctgccgat
    41521 atgaacgcgc ttcttgagtg ttcaatggaa atcggccaga tgaacttcaa agtgatgagc
    41581 attctggatg caggcgaaac cggtaaatac ggtcacccga cgccaaccca ggtcaacgtc
    41641 aaagcgacgg cgggtaaatg cattctgatt tccggtcacg atctcaaaga tctctacaac
    41701 ctgctggaac agaccgaagg cacgggcgtt aatgtctaca cccacggcga aatgctgcct
    41761 gcgcatggct acccggagct gcgtaaattc aagcatctgg tcggtaacta cggcagtggc
    41821 tggcagaatc agcaagtgga gttcgctcgt ttccctggcc ccatcgtgat gacctcgaac
    41881 tgcatcatcg acccaaccgt aggcgcttat gacgatcgta tctggacccg cagcattgtt
    41941 ggctggcctg gcgtgcgtca tctggacggt gatgatttct ctgcggttat cacccaggcg
    42001 caacagatgg caggcttccc gtacagcgaa attccgcacc ttatcaccgt gggttttggt
    42061 cgccagacgc tgcttggcgc tgctgatacg ctgattgatc tggtgagccg tgaaaaactg
    42121 cgtcatatct tcctgcttgg tggctgtgac ggcgcacgcg gcgagcgcca ctacttcacc
    42181 gatttcgcca ccagcgtgcc ggatgactgc ctgatcctga ccctcgcctg tggtaaatat
    42241 cgctttaaca aacttgagtt tggcgatatc gaaggtctgc cgcgtctggt agatgcaggt
    42301 caatgtaacg atgcttactc agcgattatt ctggctgtca ctctggcaga gaaactgggc
    42361 tgcggtgtca acgatctgcc gctgtcgctg gtgctctcct ggtttgaaca gaaagcgatc
    42421 gtcattctgc tgacgctgct ttctctgggc gtgaaaaata tcgtcaccgg cccgactgca
    42481 cctggtttcc tgacaccaga cctgctggcg gtgctgaacg agaaatttgg cctgcgttct
    42541 atcaccactg ttgaagaaga catgaagcaa ctgttgagcg cgtaaggagt ttgtcgatga
    42601 cgatgccaac gaatcaatgc ccgtggcgga tgcaggttca tcacattacg caagaaacgc
    42661 cggatgtgtg gacgatttcc ctgatttgcc acgattacta cccatatcgc gccgggcaat
    42721 atgcactggt cagcgtgcga aactcagcgg aaacgctgcg tgcttacacc atttcctcca
    42781 cgccaggcgt gagtgaatat atcaccctga ccgtgcggcg gattgatgac ggtgtcggct
    42841 cccagtggct gacgcgcgat gtaaaacgcg gtgattatct ctggctttcg gacgcgatgg
    42901 gggaatttac ctgcgacgat aaagcagaag ataaattcct gttgctggcg gcaggctgcg
    42961 gcgtcacgcc gattatgtcg atgcgtcgct ggcttgcgaa gaaccgtcca caggccgatg
    43021 tgcgggtgat ctacaacgtg cgtacgccgc aggatgttat tttcgccgat gagtggcgta
    43081 actatccggt aacgctggtg gcagaaaata acgttaccga aggctttatc gctggtcgtc
    43141 tcactcgcga actgctggca ggtgtacctg acttagcttc acgtaccgtg atgacctgcg
    43201 gcccggctcc gtatatggat tgggtagagc aggaagtgaa agcgctcggc gtgacgcgtt
    43261 tctttaaaga gaaattcttc accccagtag cagaagcggc gaccagcggt ctgaaattca
    43321 ccaaactgca accggcacga gaattttacg ccccggttgg caccacgcta ctggaggcgc
    43381 tggaaagcaa taacgttccg gttgtcgctg cctgccgtgc gggtgtttgc ggctgctgta
    43441 agacaaaagt ggtttccggt gaatatacgg tgagcagtac aatgacgctg accgacgccg
    43501 aaatcgctga aggttacgta ctggcctgct cctgccatcc gcagggggat ttggttctcg
    43561 cataatcgcc ttatgcccga tgatattcct ttcatcgggc tatttaaccg ttagtgcctc
    43621 ctttctctcc catcccttcc ccctccgtca gatgaactaa acttgttacc gttatcacat
    43681 tcaggagatg gagaaccatg aaacaaacgg ttgcagctta tatcgccaaa acactcgaat
    43741 cggcaggggt gaaacgcatc tggggagtca caggcgactc tctgaacggt cttagtgaca
    43801 gtcttaatcg catgggcacc atcgagtgga tgtccacccg ccacgaagaa gtggcggcct
    43861 ttgccgctgg cgctgaagca caacttagcg gagaactggc ggtctgcgcc ggatcgtgcg
    43921 gccccggcaa cctgcactta atcaacggcc tgttcgattg ccaccgcaat cacgttccgg
    43981 tactggcgat tgccgctcat attccctcca gcgaaattgg cagcggctat ttccaggaaa
    44041 cccacccaca agagctattc cgcgaatgta gtcactattg cgagctggtt tccagcccgg
    44101 agcagatccc acaagtactg gcgattgcca tgcgcaaagc ggtgcttaac cgtggcgttt
    44161 cggttgtcgt gttaccaggc gacgtggcgt taaaacctgc gccagaaggg gcaaccacgc
    44221 actggtatca tacgccacaa ccagtcgtga cgccggaaga agaagagtta cgcaaactgg
    44281 cgcaactgct gcgttattcc agcaatatcg ccctgatgtg tggcagcggc tgcgcggggg
    44341 cgcataaaga gttagttgag tttgccggga aaattaaagc gcctattgtt catgccctgc
    44401 gcggtaaaga acatgtcgaa tacgataatc cgtatgatgt tggaatgacc gggttaatcg
    44461 gcttctcgtc aggtttccat accatgatga acgccgacac gttagtgcta ctcggcacgc
    44521 aatttcccta ccgcgccttc tacccgaccg atgccaaaat cattcagatt gatatcaacc
    44581 cagccagcat cggcgctcac agcaaggtgg atatggcact ggtcggcgat atcaagtcga
    44641 ctctgcgtgc attgcttcca ttggtggaag aaaaagccga tcgcaagttt ctggataaag
    44701 cgctggaaga ttaccgcgac gcccgcaaag ggctggacga tttagctaaa ccgagcgaga
    44761 aagccattca cccgcaatat ctggcgcagc aaattagtca ttttgccgcc gatgacgcta
    44821 ttttcacctg tgacgttggt acgccaacgg tgtgggcggc acgttatcta aaaatgaacg
    44881 gcaagcgtcg cctgttaggt tcgtttaacc acggttcgat ggctaacgcc atgccgcagg
    44941 cgctgggtgc gcaggcgaca gagccagaac gtcaggtggt cgccatgtgc ggcgatggcg
    45001 gttttagcat gttgatgggc gatttcctct cagtagtgca gatgaaactg ccagtgaaaa
    45061 ttgtcgtctt taacaacagc gtgctgggct ttgtggcgat ggagatgaaa gctggtggct
    45121 atttgactga cggcaccgaa ctacacgaca caaactttgc ccgcattgcc gaagcgtgcg
    45181 gcattacggg tatccgtgta gaaaaagcgt ctgaagttga tgaagccctg caacgcgcct
    45241 tctctatcga cggtccggtg ttggtggatg tggtggtcgc caaagaagag ttagccattc
    45301 caccgcagat caaactcgaa caggccaaag gttttagcct gtatatgttg cgcgcaatta
    45361 tcagcggacg cggcgatgaa gtgatcgaac tggcgaaaac gaactggcta aggtaaaaag
    45421 ggtggcattt cccgtcataa taaggacatg ccatgattga tttacgcagt gataccgtta
    45481 cccgaccgag ccgcgccatg ctcgaagcga tgatggccgc cccggttggg gacgacgttt
    45541 acggagacga ccctaccgtt aatgctctgc aggactatgc agcagagctt tccggtaaag
    45601 aagccgccat ttttctgcct accggcactc aggccaacct ggtcgctctg ctcagtcact
    45661 gcgaacgtgg cgaagagtat attgtcggtc aggccgcgca taactatctg tttgaagccg
    45721 gtggcgcagc ggtgctgggc agtattcagc cgcaacccat cgacgcggct gccgacggca
    45781 cgctaccgct ggataaagtg gcgatgaaaa tcaaacccga cgatatccat ttcgcccgca
    45841 ccaaattact cagtctggaa aacacccaca acggcaaagt gctgccgcgt gaatacctga
    45901 aagaagcatg ggaatttacc cgcgagcgca atctggcgct gcatgttgac ggtgcgcgca
    45961 tctttaatgc cgtggtggct tacggctgcg aactgaaaga gatcacgcaa tattgtgatt
    46021 cgttcaccat ttgcctgtcg aaaggtcttg ggacgccagt cggttcatta ctcgtcggta
    46081 atcgtgatta cattaaacgt gccatccgct ggcggaaaat ggccggtggc gggatgcgtc
    46141 agtccgggat tctggctgcc gccgggatgt atgccctgaa aaataacgtc gcacggttgc
    46201 aggaagatca cgacaacgcc gcctggatgg cggagcaact gcgtgaagca ggcgcggatg
    46261 tgatgcgtca ggacactaat atgctgtttg ttcgcgtcgg ggaagaaaat gctgccgcgt
    46321 taggcgaata catgaaagcg agaaacgtgc tgattaacgc ctcgccgatt gtccgcctgg
    46381 tgacgcatct tgacgtctcg cgcgaacaac tggcggaagt cgccgcccac tggcgcgcat
    46441 tcctggcgcg ttaaggagag agacgtgccg caacgcattt tagttctcgg tgccagtggc
    46501 tacattggtc agcatctggt gcgcacactc agccagcaag ggcatcagat cctggcggcg
    46561 gcacgtcatg tcgacaggct tgcaaagctg caactggcaa acgtcagttg ccataaagtc
    46621 gatctcagct ggccggataa ccttccggcc ctgttgcagg atatcgatac cgtctatttt
    46681 ctggtgcaca gcatgggcga aggcggcgat tttatcgctc aggagcgcca ggtggctctc
    46741 aacgtccgcg atgcgctacg tgaagtacca gttaagcaat taatctttct cagttcgttg
    46801 caggccccgc cacatgagca gtcggatcat ctgcgtgctc gtcaggctac ggcggacatt
    46861 cttcgtgaag cgaatgtacc tgtgaccgaa cttcgggccg gaattatcgt tggcgcaggt
    46921 tcagcggcgt tcgaagtcat gcgcgatatg gtctacaacc tgccagtgtt aacgccgcca
    46981 cgctgggtac gttcacgcac cacgcccatc gcgctggaaa acttgctgca ctatctggtg
    47041 gcgttgttag atcatccggc cagcgaacac cgcatcttcg aagccgccgg accagaggtg
    47101 ctcagttatc agcaacagtt tgaacatttt atggcggtga gcggtaagcg ccgctggttg
    47161 atccccatcc ccctccccac ccgctggatt tcggtgtggt ttctcaatgt gattacttcc
    47221 gtaccgccca ccaccgccag ggcgttgatt caggggctga aacacgatct gctggcggat
    47281 gataccgcgc tacgtgcact catcccacaa cggctgatcg ctttcgatga cgcggtacgt
    47341 agcacgttga aagaggagga aaaactggtc aactccagcg actggggcta cgacgctcag
    47401 gcctttgccc gctggcgacc agagtacggt tattttgcca aacaggcggg gtttaccgtt
    47461 aaaacgtccg ccagccttgc tgctttatgg caggtagtga accaaatcgg cggtaaagag
    47521 cgttatttct ttggcaatat tttgtggcag acacgggcgt tgatggaccg cgcgatcggt
    47581 cataagctgg cgaaaggccg cccggagcgc gaatatttac agactggcga tgcggtggat
    47641 agctggaaag tgattgtcgt tgaaccggaa aaacaactta cgttgttatt tggcatgaaa
    47701 gcgccggggc tgggacgact gtgttttagc ctggaagata aaggcgacta tcgtactatc
    47761 gatgtccgcg ctttctggca tccgcacggt atgccggggc tgttttactg gttattgatg
    47821 atccccgcgc atctgtttat ttttcgcgga atggcaaaac aaatcgccag actggcagaa
    47881 caaagcacag attaatcgac aaaacctgcg ttattctttc atgtttccca ttgcatcatg
    47941 ggggaaatca cggaagaatg cgcacgttat tttctcaaca acagtggatt gatatgaagg
    48001 tactggttac cggcgccacc agcggcttag gtcgaaacgc ggtagagttt ttatgccaga
    48061 aaggcatcag cgtgcgagcg accggtcgca acgaggcaat gggcaaattg ctggaaaaaa
    48121 tgggcgcaga gtttgttccg gcggatctga ccgagctggt ttcatcacaa gctaaagtga
    48181 tgctcgcggg cattgatacg ctgtggcact gctccagctt tacctcaccc tgggggacac
    48241 aacaggcttt cgatctggct aacgttcgcg ccactcgccg cctgggtgaa tggactgtcg
    48301 cctggggtgt gcgtaacttt attcatatct cttccccctc cctgtacttc gattatcacc
    48361 accatcgcga tattaaagaa gattttcgcc ctcaccgctt cgccaacgag tttgcccgca
    48421 gcaaagcagc cagcgaagaa gtgatcaata tgctttcgca ggcgaatcca caaacgcgct
    48481 ttactattct gcgcccacaa agtctgttcg gaccgcacga taaagtcttt attccccgtc
    48541 tggcgcatat gatgcaccac tacggcagta ttctgttacc gcatggcggc agtgcgctgg
    48601 tcgatatgac ctactatgaa aatgccgtgc acgcaatgtg gctggcaagc caggaagcct
    48661 gcgataagct accttccggg cgtgtgtaca acatcaccaa cggcgagcat cgcacactgc
    48721 gcagcatcgt gcagaagctg atcgacgagt tgaatattga ctgtcgtatt cgttccgtcc
    48781 cctacccgat gctggatatg atcgcccgca gcatggagcg tttaggccgc aagtcagcaa
    48841 aagagccgcc gctgacccac tacggcgtct ccaagttgaa ttttgacttt acgctggata
    48901 ttacgcgtgc gcaggaagag ttaggttatc agccggtcat caccctggat gaaggtatcg
    48961 agaaaactgc cgcctggcta cgcgaccacg gaaaactgcc gcgctaatcc tgcccgtatt
    49021 tctccagcaa tgcttcggca atcgcctgag tttctgcatc cgcttcgccg ttatataacg
    49081 tcgggcggaa atgcatctgg aatgccataa tcacgcgccg ctgctcgcgc ggtgtcatat
    49141 caggtttaac gtcataaccg tagcgcgcca aaagctccag caatgacgcg gtatctaccg
    49201 gtgtgtgcgg cgcgcgcccg gcaaggtaaa agttaacccg ctgcgcatcc ggccaggcac
    49261 caatcccctg ctgcgccagt tgctgccagg gaaataatgg ccccggatcg tctttgcgct
    49321 gcggtgcgat atccgcatgt gccactacgt tttccggctt gatgtgataa cgggcaataa
    49381 tatctttcac cagtggaata agcgcctgaa tctgtgccgg ttcaaacggg gcaaaatatt
    49441 tcactccggc cgatttttgc cagccacggt tttccagctc aatgccaata gaggtgtcgt
    49501 taaggcgcgt tgccccgcgc caggcgctaa tccccgcatg ccaggccagt tcttgttccg
    49561 gcaccagttg ccagatgcgc ggtttaccgt tgtatcgcgg tggtaccgca gggaccagat
    49621 aatgcgagct gacctgctta tcggtcagtg tcgccagcga gctatcaaaa tcatctgcgg
    49681 tgtagtggat caccagcact ttaatgcgcg gatacgccgc ctgcgcctgg cgtcgggtat
    49741 caagctgata tccctctttc tcgacaatgc ctttttcgcc tgcacaccct gccagtaaca
    49801 gagcggcagc gatcagccag aagactcttc tcatcgacgc gttttcaccg ccgtaccgct
    49861 gacgctaacc atcagcatac tgccgttttg cccgaccgtt tcgtagtcga tatcaatacc
    49921 gacgacggca tcggccccca gcgcccgcgc ctgggagcct aattcctcaa aggcgatctc
    49981 ccgtgcttta cgcagttctt tttcatacgc accggaacgt ccgccaacga tatcgcggat
    50041 accggcaaag aaatcacgga aaatattggc acctaaaatc gcttcgccgg tcaccacacc
    50101 gcaatattca acgatgatct ggccttccag agttggggtt gtagaaaatt gcatgatcga
    50161 tctccttgtt tgttaagcct tcgatctcta aagcattatc agactgatac gctattattg
    50221 aaaggatatc attattatcg tagaacgctt tcagagcgat cgcttataag gaaatcatta
    50281 tgcgctacag caaattgaca atgcttatcc cctgcgcact gctcctcagt gcctgcacca
    50341 cagtcactcc agcttataaa gataacggca cacgcagtgg tccttgcgtt gaaggaggcc
    50401 cggataacgt ggcccagcaa ttttatgact accgcatttt gcaccgcagc aacgacatca
    50461 ccgccttgcg cccttatctg agcgacaaac tggcgacact gcttagcgat gcctcccggg
    50521 ataataacca tcgggaacta ctgaccaacg atccattctc cagtcgcaca accttgccag
    50581 agagcgcccg tgttgccagt gcctcaacaa tcccgaatcg tgatgcgcga aatattcctc
    50641 tgcgtgtgga tctaaaacag ggtgatcaga gctggcagga tgaagtgtta atgattcagg
    50701 aaggccagtg ttgggtgatc gacgatgtac gctatttagg cggcagcgtt catgccacgg
    50761 cgggtacgct ccgtcagtcc atcgagaatc gctaacgcct tgatcaacca ataaaccccg
    50821 taaattgtcc ggcatttcgt gagaaatgcc gacatttatg ctcgccgacc accgcccccg
    50881 ttattttgtg ctatgtttat tgaataatgc gctttgcttt taacttttaa agcagaaata
    50941 ttgcataatt attctgtcaa aggtactatc ttcgacctca atagctatca gactgccagt
    51001 atacgagtgt caatgagtat tcaattaaac ggcataaatt gcttctacgg cgcgcatcag
    51061 gcgctgttcg atatcacgct ggattgccca cagggcgaaa cgctggtgtt acttggcccc
    51121 agcggcgcgg gtaaaagctc gctgctgcgt gtactcaatc tgcttgagat gccgcgctcc
    51181 ggtacgctca acattgcagg caaccatttc gatttcacca aaacaccctc tgacaaagcg
    51241 attcgcgatt tgcgtcgtaa cgttggcatg gtgtttcagc aatacaacct gtggccgcat
    51301 ctgaccgtgc agcaaaacct gattgaagcg ccctgccgtg tactggggtt gagtaaagat
    51361 caggcgctgg cccgtgcaga aaaactgctg gaacgtctgc gtctcaaacc ttatagcgat
    51421 cgttacccgc tgcatctttc tggtggtcag cagcagcgtg ttgctattgc ccgtgcgttg
    51481 atgatggaac cgcaggtact gctgttcgat gaaccgaccg ccgcactgga cccggaaatt
    51541 acggcacaaa tcgtcagcat cattcgtgag ctggcagaaa cgaatattac ccaggtgatc
    51601 gtcacccacg aagttgaagt ggcgcgtaaa accgccagcc gagtggtgta tatggaaaat
    51661 ggtcatatcg tagaacaagg cgacgcgagc tgctttaccg agccgcaaac cgaagcattt
    51721 aaaaactatc tctctcacta agattcggga taacaacaat gaaaaaagtt ctgattgccg
    51781 cgttaattgc aggttttagt ctttccgcca cagctgccga aaccattcgt tttgctaccg
    51841 aagcatccta tcctccgttt gaatcgattg atgcaaacaa ccagatcgtt ggttttgacg
    51901 tcgacctggc acaagcgctg tgtaaagaga ttgatgcaac ctgcactttc tctaaccagg
    51961 cgtttgacag cctgatccca agcctgaaat tccgtcgcgt agaagccgtg atggcgggca
    52021 tggatatcac tccggagcgt gaaaagcagg tgctgtttac caccccgtac tatgacaact
    52081 ctgccctgtt tgtgggtcag caaggcaaat acaccagtgt tgatcagctg aaaggcaaaa
    52141 aagtcggcgt acagaacggg acgacacacc agaaattcat tatggataag cacccggaaa
    52201 tcactaccgt tccgtatgac agctaccaga acgcaaaact ggatctgcaa aacgggcgta
    52261 tcgacggcgt cttcggtgac accgcagtgg tcactgagtg gctgaaagat aacccgaaac
    52321 tggcggcggt gggcgacaaa gtgaccgata aagattactt cggcactggc ctcggcatcg
    52381 cggtacgtca gggcaacact gagctgcagc agaaactcaa cactgcgctg gaaaaagtga
    52441 agaaagatgg cacttacgaa accatctaca acaaatggtt ccagaagtaa ttcctgatga
    52501 atgaattttt tcctttagca agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat
    52561 tgattgtcgg gctggcgctg gcgatgttct ttgcggtatg ggagtcggca aaatggcgtc
    52621 ctgtcgcgtg ggcaggttca gcgctggtaa ccattctgcg tggcctgcca gaaattctgg
    52681 tggtgctgtt tatctatttt ggctcctcgc agctgctgct gacgctttcg gatggcttca
    52741 ctatcaatct tgggttcgtg cagatcccag tgcagatgga cattgagaac ttcgacgtta
    52801 gcccgttcct ttgtggtgtc atcgctctgt cactgctgta tgccgcctat gcctcgcaaa
    52861 cgcttcgggg cgcgttgaaa gcggtgccgg tgggtcagtg ggagtctggt caggcgctgg
    52921 ggctgtcgaa atcggctatc tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc
    52981 tgcctggcct cggtaaccag tggctggtgc tgctgaaaga taccgcgctg gtcagtttga
    53041 ttagtgtgaa tgatttaatg ctgcaaacaa aaagcatcgc tactcgtacc caggaaccat
    53101 ttacctggta cattgtggcg gcggcgattt acctggtgat caccctgctc agtcagtaca
    53161 ttctcaaacg cattgacctg cgcgcgacac gttttgagcg gaggcccagc taatgtttga
    53221 gtatttaccc gaactgatga aagggctaca caccagcctg acgctaaccg ttgcctcgct
    53281 gattgtggca ctgattctgg cattgatttt taccatcatc ctgacgctga aaacgccggt
    53341 gctggtgtgg ctggtgcggg gttatatcac gctgtttacc ggtacgccgc tgctggtgca
    53401 gatcttcctg atttattacg ggccgggcca gtttccgact ttgcaggagt atccggcact
    53461 gtggcatttg ttgtcagaac cgtggttatg tgcgctgatt gcgttatcgc tgaacagtgc
    53521 ggcgtatacc acgcagctgt tttacggtgc aattcgtgcg atcccagaag gtcagtggca
    53581 gtcctgtagc gccctgggaa tgagcaaaaa agatacgctg gcgatcctgc tgccgtatgc
    53641 ctttaaacgc tcgctctctt cttattccaa cgaagtggtg ctggtgttca aaagtacctc
    53701 tctggcttac accattacgc tgatggaagt gatgggatac agccagttgt tgtacggacg
    53761 cacctacgat gtaatggtgt tcggtgcggc agggattatt tacctggtcg tcaacggcct
    53821 gctgacgctg atgatgcgtc tgatcgagcg caaagcactg gcattcgaac ggcgaaatta
    53881 atgtagctgc gtacattcgc cggggggggt ttgtaggtct gataagacgc gccagcgtcg
    53941 catcagacat cagcacggtg cctgtgccgg atgcggcgtg gacgccttat ccggcctaca
    54001 tagtgcataa attcttataa tgaagacgga caactcacta aggtatccgt ctttttttat
    54061 ttcatttaaa ttatttaatc atatttattg catataaatt cacttgatgg cattgttatc
    54121 ccatgccgca gacacggcca aaaatcataa gattgacaga cgggagttcc atcatgaaaa
    54181 agttagttct tgccgcttta cttgcttcct ttactttcgg tgcttctgcc gcagagaaaa
    54241 tcaattttgg cgtttcagcc acctatccac cctttgaatc tataggtgct aataatgaga
    54301 ttgtcggctt tgatatcgat ctggcaaaag ccttgtgcaa acaaatgcag gcagaatgta
    54361 cttttactaa tcacgcgttc gacagcctga tcccgtcact gaaattcaga aaatatgacg
    54421 ccgtaatctc cggtatggat atcacgcccg agcgtagcaa gcaggtatcg tttaccacgc
    54481 cctactatga aaactcagcc gtcgtgattg ccaaaaaaga tacctacaaa acgtttgccg
    54541 atctgaaagg caaacgtatt gggatggaaa acggcaccac gcaccagaaa tatattcagg
    54601 atcagcaccc cgaagtgaaa actgtctcct atgacagtta tcagaatgcc tttatcgatc
    54661 tgaaaaatgg tcgtattgat ggggtatttg gtgacacagc ggtggtaaac gaatggctga
    54721 aaaccaatcc gcaactgggc gttgctactg aaaaagtgac cgatccgcaa tacttcggca
    54781 ccggtctggg catcgctgta cgtccggata acaaagctct gctggaaaaa ctgaataacg
    54841 cgctggcagc aattaaagct gacggtacat atcagaaaat caatgaccag tggtttccgc
    54901 agtaattgag actgatgaca aaagtaagat tgcctgatgc gctgcgctta tccggcccac
    54961 atcaactctg caatgtattg atttagcatg attttgtagg ccggataagg cgtacacgcc
    55021 gcatccggca tgaacaaagc gcacctgatc agcaatctga agccgcacct gcggcttttt
    55081 tattgcttca ccagcagcgt cagcacttca tagtgcgcgg tgtgcgggaa catatcaaaa
    55141 agctgtaccc gttcgatgcg gtaacctggc agttcgcgga tatctttcgc catggtttgg
    55201 gcgttacagc tggagtagat aataaaacgc ggtgccattg ttgagagata atcacatagc
    55261 ggtttaccaa tgccgcggcg cggcggatta accagcacca gctccggcac ctccccctga
    55321 gcggtggcaa actgagtgga gtccagcgcc tgaaattgca aacgcgttaa gcccagttca
    55381 gcggctgact gctttgcaca ggcaatggcc tctgatgcaa tttcgatccc ggttaactgc
    55441 atgtcaggcg tcgcgcagtg taaaccaaag ccacccacac cgcagaacag atcccacata
    55501 tgtttaactg gcagctgccg tacccagtcg cgcgcggtag cgtacagctg gctggcgacc
    55561 gccggattag tctggaagaa actttgcgga cggatccaca gcggtacgtc attaaaacgc
    55621 tctgccagcg cctgttgttc agtaaggtag atctccgtct ccccttccat aatcgccata
    55681 tgtaccggct gaatattgac ggtaataact ttcagttgcg gtagttgttc ctgtaaccac
    55741 ggcagcgcct tacgcagttg tgccagtttg gtttcagaac gcagtacaaa gcgcagcatc
    55801 atgccgccat cgctctggct ttcagtcagc agaatgtatt tcagttcgcc acgcttacgc
    55861 gccacgttgt agggtgtcag ccccgcacgg gcgataaacg gttttagcgc cgcaaaaacg
    55921 ggcgcaaatg aggcaggata aagcgggcag tcacaaaggt cttctggtgt gccgtcgcga
    55981 tgcagcatac cgagcagtgg tttttcaacg ctaccactca ccaccatttt ggctttatta
    56041 cgaaaccctt gttccgggcc tgacaccggc gcgcaccatt cctcaaccgg aaaatcggcg
    56101 agcagatttt taagatcggc ggttttagcg gagagttgct ctggaatcgg ctgcgttatc
    56161 cactgacagg aacgacagcg acccgcgtcg taaagtgcgc actgcataca ttgaccttca
    56221 catcatcagg ggcgacgatt atacacatta ttgcaactgg aagaaccgcc gactggtcga
    56281 gggaacgaac agcagcatca ggatgagcat atccggcagc ttctgcagca tcaggctatg
    56341 gaagatttca cgttttgatt caccgggaat gctgaacagc tccggataac cgtaccccag
    56401 cgaagccgcc cacaagtaac ttgcggcggt gatttgtgtc agcagatata gccagcgcgc
    56461 ccagcgacgc cctttcacca gtgaaaacgc acaccagatc tcaatgaaaa ccagcaccag
    56521 actgcttaaa aagaccagcg ttaaactcca ggtttgtacg ctgcgatgaa tgaactcgcc
    56581 aataccgcgc acgcccagcg tattgaaaat catcagcacg tcgaggccac ggatcataat
    56641 aatggcgagc gccgccacct gcaccagcgc aggcacattc aggcgagcat gagatgaaga
    56701 tgttttctta aaaaatccca acgtttcgtc ttccatgaaa acaatgccgc ggcatgcgcg
    56761 gcattatgta gccaggttgg caaattttag tgtcttcagc catgtcttgc acgctggata
    56821 tcgcgtatcc gctgtttttc tgcgcgagcc atcagatacc aggcgataaa tccgacaatt
    56881 ccgaccgcac cgagaattaa cgttgcaagg gcgttgattt ccggattcac ccccatccgc
    56941 acgctggaaa agaccagcat cggtaacgtg gtggctcccg gcccggaaac aaagctggcg
    57001 atcaccagat catcaagcga caaagtaaaa gccagtaacc agccagaaat gatcgcgggc
    57061 atgatcatcg gtagcgtaat gacgaaaaac actttcagcg gcgtcgcacc gagatccatc
    57121 gctgcttctt ctatcgagcg atccagttcc cgcaatcttg aagaaataac gaccgccaca
    57181 taagccgtac aaaaagtgac atgcgccagc cagatggtga gcataccgcg gtccgcaggc
    57241 cagccaatgg catgagcaag cgcgacgaat aataacaaca gcgacaagcc cgtgatgaca
    57301 tctggcatca ccagcggcgc ggtgatcata aaggcaaagc catttgatcc gcgaaacctg
    57361 ccaaagcgca ccagcaccac cgccgcaata gtcccgagga tcgccgccgc cgttgccgca
    57421 caggcagcaa ttgtcaggct taaaccaacc gcactcatca tcgcgtcatc gcgcaataac
    57481 tcaccatacc agcgcgttga ccagccggcc cacaccgtca ccagcttcga gctgttaaac
    57541 gaatagataa ccagcatcag cattggcgcg tagagaaagg taaagcccag cagcaaaatc
    57601 acaatccgcc agggcgaacg aactaccggt aaattattca tccgtgttct cccacgcttt
    57661 tttgctggtg tttgtggaac cacattatcg gcacaattag cagcagcaac atgatgatcg
    57721 ctaccgccga ggccaccggc cagtcgcggt tattgaaaaa ctcttgccat agcacgcgcc
    57781 cgatcatgat gctgtccggg ccaccgagca gttccgggat cacaaactcg cccacagccg
    57841 ggataaacac cagcatcgat ccggcaataa tcccaccttt agtcagcggc acgatcacag
    57901 taaagaacgt tttcagcggt cgtgcaccga gatccagcgc tgcttccacc agcgaataat
    57961 caatacgaat caacgcggta taaatcggca gtaccataaa cggcacgtag gcgtaaacaa
    58021 tgccaatata aactgccaga ttggtatgaa gaatggtcag cggttgatcg ataaccccca
    58081 gccacagcag aaaattattc agcacaccgt tgttttttaa tattcccatc caggcataaa
    58141 cgcggatcag aaacgaggtc cacgacggca ggatcaccag tagtaataaa atattacggg
    58201 tcgaaggctt actgtgcgcc accgcccacg ccagcggata gccgatcagt aaacagcaaa
    58261 atgtcgaaat cgcagccacc tgtagctact ggagataagc atcgaaatag agcggatcgt
    58321 cggtcagttg cagaaaatta ccgagattaa gagtgatgga cagttgcccg tcagcccact
    58381 ccatcagttc ggtataaggt ggaatagcgc gcgccatctc cgccaggctt attttaaaga
    58441 caatcagaaa tggcagcaga aacagcaaga tcaaccagat atatggcaac gcaatgacca
    58501 gtttgcgccc atgcttcatt tgcagctgcg acaaccacag cttaaatccg cccggcggtt
    58561 tcgactgggc agcaggttca agtgtattca tctttgctcc ttaaaccgtc agcaccacac
    58621 agctgtccac ttcccagcac aaacgcactt cgtcgcccca ggtcggtaac cctttacgat
    58681 gacgatgggc gttttgtagc tgggcgctaa tcatctgccc acttttcaga cgaacgtgat
    58741 acaccgaaag atcgccgaga taggcaatgt gtatcacctc ccccaccgcg aagttacaac
    58801 cattggcggg cggctcttcg caaagcatga ttttttccgg gcgcagcgct acgtgtaccg
    58861 gcacgttatc gaccactgaa gcatccgcat cgactttcag tggatgcacc agccccggcg
    58921 aatcaagcac caggccatct tcctgacgct ctttgagtac accttcaaag acattaaccg
    58981 aaccgataaa ttcagcgctg tagcgggtag ttggatgctc gtagatctct tccggctcgc
    59041 caatctggac aaatttcccg cgattcataa tggcgatacg ccccgccatg gtcatcgcct
    59101 cttcctgatc gtgggtgacc atcacacaag tcacaccgac gcgctccaga atatccacca
    59161 cttcaagctg catccggtca cgcagctttt tatccagcgc gcccatcggc tcatcgagca
    59221 gtaatagttt cgggcgtttg gcaaggcttc gggccagggc cacacgctgt cgctgaccac
    59281 cggaaagctg atgcggtttg cgtttggcga actcctgcat gtgcaccagc ccaagcatct
    59341 cgttgacccg ggtggcaatt tccgctttcg gtagtttgtc ctgtttcagg ccaaaggcga
    59401 tgttctgctc cacagtcata tggggaaaca gcgcgtaaga ctgaaacatc atattgatgg
    59461 ggcgcaggta aggcggcacc tgtgacaaat cgacgccatc aagcattatc tgtccggcag
    59521 aaggttgttc gaaacctgcc agcatacgca gcagcgtgga cttgccacag ccggatgcgc
    59581 ccagcagcgc gaagatttca cctttgtaga tggtcaggct gacatcatcc accgcatgtt
    59641 gaccatcgta ggatttggtg aggttgcgga tttctaatag cggcgtcagc gccttacggg
    59701 ttttcgcctg cgggcgaggg atagcgtcat tcacggctgc tctccggcat tgaatgtgtg
    59761 caagcgcacc atgatggtgc gaattgccgg cgggtgtggt gcgcccgccc ggcatctaag
    59821 gctgcggatt attttccgct cttcactttg gtccacgcgc gggtgcgcac acggtcgatt
    59881 ttcgggtcct gcactttcag agtgaacagc ttcgcacgaa catccgcagg cggataaata
    59941 cccgggttct cacggacttc cgcacccacc agcggcgtgg ctgctttatt ggcgttggca
    60001 tagaacacat tgtcggaaat atgcgctact acatccgggc gcagcaggta attcaggaac
    60061 tgataggctt cgtctttgtt tttggcatcc gcaggcatgg cgaatacatc aaagaacgcc
    60121 atcgcccctt cttttggaat cgagaacgag acattcactc cattcttcgc ttccttcgcg
    60181 cggtttgacg cctgccagac atcacctgcc cagccgatag cgacgcaaat atcgccgttt
    60241 gccaggtcgt taatgtattg agatgaatgg aaataacgaa tgttcgggcg cagctttaac
    60301 agcagatcgg ttgccggtcc ggtgtaatca tccgctttag tgctgttggg atctttgccg
    60361 agataattca acacggtagc aaaaacttct tctggcgcat ccaggaaaga gacaccgcag
    60421 cttttcagtt tttccagatt ttcaggtttg aggatcaagt cccagctatc gacgggcgcg
    60481 ttttcgccca gcacagcttt aactttatca acgttatagc caatcccggt cgtcgcccac
    60541 atatagggca tagcaaattt attgtcggga tcgtgtttgg cgaccagctt cagcagctcc
    60601 gggtcgagat tcttccactc cggcaatttg cttttgtcca gcggctggaa aactcccgca
    60661 gtcaactggc gttcaagaaa gctggcagat ggaaccacca agtcaaagcc ggtgctaccc
    60721 gccattaatt tgccttccag tacttcgtta gagtcgaaaa catcgtagac gactttaata
    60781 ccggtttctt tttcaaaatt ggccaccgtg tccggggcga tataatcaga ccagttataa
    60841 acgtggagtg ttttttgttc agccgcgagc gtgccgacag agacggccat cagagcaccc
    60901 gcagccagac ccgaaagcca ttttttattt aaggcggtca tatctctttc cttctgaaag
    60961 ttcgttaaca aacgaacaaa agttgtcaca ctaaaagata tgcaataaac gtgcatattt
    61021 tttatcggcg gggtgtaaga aaagagaact ctctcccggc cagcactgct cgctatataa
    61081 aagcatcgca agaataactt tagccagggt ttgagactat agctcggatt aaaaaacggt
    61141 atttaacaaa tttttatgcg tatttgctct atgtgataaa gccaaatcga cacaagagaa
    61201 gtgaaataat ctttaagaaa aggttaaaaa cagcagataa ttttgcagcg cgcagccact
    61261 gcaccagcga ctgcgtgctc gaaaggagtt atcagtggag gaaatagttt tcggtaacgt
    61321 tgttttgccc ttcatcgtca gtcggtaaca gcagttgatg ggcattagct tcaagaatga
    61381 ccatcaaaat ctgctcttcg ctctgacgca cgaaccatgc aaactgctca taggttacgc
    61441 cctgcataac ggataaagac tggcaaacca ccagctttgg cagattatca tcctgcatat
    61501 caagaaatgc tttcacggtc agcgaactgg cattgatggc agataaatcc gccgccagcg
    61561 gcaatacggc tgaaggtcgg acttccgcca tggcagaaaa caggatcgtg ttatcgatca
    61621 gatcgatttt ggcatcaaag acaccgtcga aattctgcat atggggcaga tgcagagcct
    61681 gacagttatc acattcaaaa aaactcatcc ccaggtcatc gagccattga cgcagcgtat
    61741 ccagaccagg aacgaccagc gatgtcataa ttttgtacaa cctctttcga taaaaagacc
    61801 ggcacagctt acgcaaaaag cgcaggcaaa accatgatca gtaatgtgat tgcgactaac
    61861 cacccgtttt caggcaatat tctgtcgtag cgtggcgttc gatccagcgg atcattttac
    61921 ccgcgatgtc gataccggtg gttttttcta ttccttccag ccccggcgac gcattcacct
    61981 ccatcaccaa cggcccgcga ttagcacgca gaatatccac accagcaacg tccagcgcca
    62041 tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg tgtgatactg gcgacgcttg
    62101 ccgcgccgcc acgatgcaaa ttggaacgaa aatcgccctc tttcgcccgc cgttcaatcg
    62161 cagcgaccac ttcatcgcca acaaccagac agcggatatc gcacccttgc gcctctttga
    62221 tatattcctg caccagaata tgcgcgttca gaccgcggaa agcgtcaatc acgctttccg
    62281 ccgcctgacg cgtctccgcc agcacgacgc caattccctg cgtgccttca accaacttga
    62341 ccaccagcgg cgcaccaccg accatgtcga ttaaatcgct ggtatcatcc ggcgaatgcg
    62401 caatgcccgt gacaggcagg tcgatgccct gacgcgccag cagttgcatg gaacgcaatt
    62461 tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg atagctcccc agcatctcga
    62521 actggcgcag tgccgccgtc ccataaaagg taatggcggt gccaatacgc gggatcactg
    62581 cgtcaaaatg gggtaacttg cggcctttgt agtgaataga agacgccgca ggatttatgt
    62641 tcatgtagca agaaagcgga tcaagaattt caaccaggtg accgcgctgt atagcggctt
    62701 cacgcagccg cttacacgaa tagagcgttc catcccggga caatatggca attttcaccc
    62761 tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca tacatcgacg tggcggtttt
    62821 agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat aaatgggcgg ctttctttaa
    62881 tgattgttcg gcggatatga tcgctccagg tatcccggcg attattgctg ccacgggtga
    62941 ggtaatattc cgccagttgc tcgtcatact gcgccagtgc gcctttatcc agcggttgat
    63001 agctgttttc atgcaccaaa atggaggccg gtaaacgcgg cttaagatcc ggattatccg
    63061 caggccagcc aaggcacagc ccaaacagcg gcagaacatg ctgcggtaat ttaagcagtt
    63121 tcgtcaccgc ttcaatatta ttgcgcaggc cgccgatata taccccgccc aatcccagcg
    63181 attccgctgc gattaatgca ttctgcgcca tcattgccgt atcaacgaca ccgagcaaca
    63241 gttgttccgc caggccgagc tgagcatccg gacagatctg taaatggcgg ttaaagtcgg
    63301 cacagaacac ccagaactcc gccgcttgcg ctacgtgttt ttgcccgccg gtcagcgtca
    63361 ccagttcttc acgtaacgct ttgtcggtaa tgcgaataat gctactgcac tgcaaaaaac
    63421 tggaactgga cgtcgcacgg gcgctgttaa taatcgcctc acgctgcgct tcggaaatgg
    63481 gttcatcagt gaaatggcga atggagcgat ggccacaaat aagttcaatg gttggcgtca
    63541 ttatcttttt ctctttctga acgtgaatat tgcggtggac ggttcatcag ctgtggggca
    63601 agacgttttg ccacctgaag aataaccacc accgcagcgg gaagcatgag caaaacaccg
    63661 agaaaaatca tcagaatctg cacttctggc cgagaaaatg gctcaggcag cgacagggag
    63721 tcgcttaccg acagcagcgc caccgccagt agcatcattc cgataaattc cagtatcaac
    63781 acgcctttag gcaatttacc gatcgcgcgc atacgcttcc ctctgcaaag tgagccttca
    63841 gtctaaaact tttcactgta ttgtgtttaa cagttatagc ttttagcaat taatgcaaca
    63901 ggttaaacct actttcagcg aatacatttt agcgtgatca ttacaggcat aaatctatga
    63961 ggagagaaat aatgcaaacc gttatttttg gtcgttcggg ttgcccttac tgtgtgcgtg
    64021 caaaagatct ggctgagaaa ttgagcaatg aacgcgatga ttttcagtat cagtatgtag
    64081 atattcgtgc ggaagggatc actaaagaag atctacaaca aaaggcaggt aaacccgtag
    64141 aaaccgtgcc gcagattttt gtcgatcagc aacatatcgg cggctatacc gattttgctg
    64201 catgggtgaa agaaaatctg gacgcctgat cgtctgacaa gccctcgcgt tgagggcttt
    64261 actgattttt tctgtgctgt ggtttaaaca aactactgat aaataagaaa cacagtgccc
    64321 ccagcgcaca ccagaacacc gcgcttagta accatgccag ctcttgccag aatgagcgcg
    64381 tcggtgaaaa aaacagccgc ataatgagca ttgaacaggg tgccgccagc attgcgccaa
    64441 atagaggttt caggacttct ctacgctgtg aaaagaagct ggcgactgct ccaggaagaa
    64501 tgaaaaatag caagccgatt tcaggatgcc cggcagcccg aaaagcgcct ttcatgtgcg
    64561 tcgccagaaa aaggcacacc acaatgaaga ggacaaaaca gcagattgcc cccgcccaac
    64621 gttgtttatg tttcactcgt tcctcctgac actgcgtcta tcgaacacat ttttcgccag
    64681 tgtggcgttc agtaagataa agccgcttcg cattccatgc taatataggc caacgcaatt
    64741 catatagccg ttgataccta atgtgattac actagtaaaa tatattgtta ctttactatc
    64801 gtttaggtgc gctgaatgaa tctgcgccct gaattctggt aaaaaacatt atcgtaaatt
    64861 accatttctt tcaacagttt actagtaaac aagaagttag cctccgtgaa tataaacgtc
    64921 gccgaattgt taaatgggaa ttacattctg ttattatttg tggtcctcgc gcttgggcta
    64981 tgtctcggaa agttacgact tggttcgatc caactgggta attccattgg cgttttagtc
    65041 gtatcgctgt tattaggcca acaacatttc agcattaaca ccgatgcgct taatcttggc
    65101 tttatgctgt ttattttctg cgtcggggtc gaagccggac cgaacttttt ttccattttt
    65161 tttcgcgatg ggaaaaatta cctaatgtta gcactggtga tggttggcag tgcgctggtg
    65221 atcgccttag ggttaggtaa gctgtttggc tgggatattg gcctgacggc cggtatgtta
    65281 gcaggctcta tgacgtcgac accggttctg gtcggtgctg gcgatacact gcgtcattcc
    65341 ggcatggaaa gcaggcagct ctcactggca ctggataatc tgagcctcgg gtatgcctta
    65401 acctatttaa tcggtctggt gagtttgatt gttggtgcgc gttacttgcc gaaattgcag
    65461 catcaggact tacagaccag cgcccagcaa atcgcccgcg aacgtggcct ggacactgat
    65521 gccaaccgta aggtttattt accggtgatc cgcgcctatc gcgtcggccc ggagctggtg
    65581 gcctggaccg acggcaaaaa tctgcgtgaa ctgggtattt atcgacaaac cggctgctac
    65641 attgaacgta ttcgacgtaa cgggattctg gcaaatccag acggtgatgc cgtgctacaa
    65701 atgggcgatg aaatagcgtt ggtaggctat cccgacgccc atgcccgact cgatcccagc
    65761 ttccgtaacg gtaaagaagt tttcgatcgt gaccttctcg acatgcgtat cgtcactgaa
    65821 gaagtggtcg ttaaaaacca taacgctgta ggtaaacgtc tcgcacaact gaagttgacc
    65881 gatcacggtt gcttccttaa ccgcgtcatt cgtagccaga ttgagatgcc gatagatgac
    65941 aacgtcgtgc ttaacaaagg tgacgtttta caagtcagcg gtgatgcccg tcgcgtaaaa
    66001 accatcgccg atcgcatcgg ctttatctcg attcacagcc aggtcactga cctgctggca
    66061 ttttgcgcct tctttgttat tgggctgatg atcgggatga tcaccttcca gttcagcaca
    66121 ttcagtttcg gcatggggaa cgctgccggg ttgttattcg ccggaattat gctgggcttt
    66181 atgcgtgcta accacccgac cttcggttac attccgcaag gtgcattaag catggtgaaa
    66241 gagttcggct tgatggtgtt tatggcaggc gttggtctga gcgccggtag cggtattaat
    66301 aacggcctgg gcgcgattgg cggtcagatg ttgattgccg gattaattgt cagtcttgtg
    66361 cccgtggtta tctgtttctt gttcggtgct tatgtattgc gaatgaaccg cgcactgttg
    66421 ttcggcgcaa tgatgggcgc acgcacctgc gcgccggcaa tggagatcat cagtgataca
    66481 gctcgcagta acatccctgc gctgggctat gcgggcacct acgcaatcgc caacgtcctg
    66541 ctgacgctgg cagggacaat catcgtcatg gtatggccag gattaggata aaactgaagt
    66601 tgccctgaaa atgaaatttt tttgcacaac cgcagaactt ttccgcaggg catcagtctt
    66661 aattagtgcc actgcttttc tttgatgtcc ccattttgtg gagcccatca accccgccat
    66721 ttcggttcaa ggttgatggg ttttttgtta tctaaaattt atctactacc ctgcaaccct
    66781 ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga caaaatgcag
    66841 cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa accattgttc
    66901 gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata gcggttttcg
    66961 gctagccagc gcgtagagct ggtacatcag ctccatgtta tccggcgttg caacctgtga
    67021 gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa catcgctaaa
    67081 aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct ccagcaacaa
    67141 ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg caagggtagc
    67201 aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca gcgtggcctg
    67261 gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc ctctgtctgc
    67321 aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt attttgacgt
    67381 gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg gcgctgttta
    67441 tgttcttctt tttgccgggc ctgttaatgg cgtcctgggc aacccgtacg cctgctatcc
    67501 gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt ctgtcgatcg
    67561 gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg acacgtaatg
    67621 tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt ctggcactca
    67681 ggctgacatc acccctgctc ttcgcggttg gcctcggcgt ctttggggca agttttggtt
    67741 ctgcggaagt ggcgataaac gtcgaaggtg ccgccgttga gcgagaaatg aataaaacgg
    67801 ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct ggtgtcggga
    67861 tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg gcgctggtag
    67921 gaatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc aaaaatgctg
    67981 ccgatggcac ccagcatggt gaaaaaggcg taccttttta tcgcgatatc cagttgctgc
    68041 tgattggtgt tgtggtgctg gcgatggcct ttgccgaagg ttctgccaac gactagttac
    68101 ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg atttatgccg
    68161 gttttaccct ggggatgact gttggacgct ttaccggcgg ttggttcatc gaccgttaca
    68221 gtcgcgttgc cgtggttcgg gccagtgcac taatgggagc gttgggtatt gggctgatta
    68281 tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga ctgggtgcct
    68341 cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat gcaccaaccc
    68401 gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg ccgctgctgg
    68461 gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg gcgctggtta
    68521 ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg cagacggcga
    68581 tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta ctttcttaag
    68641 cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa tgaaagcaca
    68701 aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct ctttcttccc
    68761 tgaaattgcg aatgaaattg cctttgtggc tgaaaacggc ggctgggtag tgagtgaagg
    68821 caaagatgtt tttaatggcg agctgtcgaa ggatgcgttt gctactgtcg tggaacattt
    68881 gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct atacactcaa
    68941 aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc tggaatacgt
    69001 cgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc tttccgatga
    69061 actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta tggtgccagt
    69121 ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag ccaatggcct
    69181 tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct ttggcgatgg
    69241 cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg aaaatgccgg
    69301 cagcgcggtc gtcgcagcgg caaaataccg cgcaggctcc aataaccgtg aaggcgtact
    69361 ggatgtgatc gataaagttc ttaaacacga agcgccattt gaccaataaa aacggcagca
    69421 cgcccggcaa cacaatgcct gaatgaaggc tgcataattc gcggaaatta acccgctgca
    69481 ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt ttaatgggct
    69541 ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga tacaaagtgg
    69601 ttgtggttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact gtgccgcaaa
    69661 ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat gttcgccatc
    69721 ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat caccattaat
    69781 tcgagaatgc ctgatcgcac aaagcaatca ggcatttttt tacccttcgt gagaatttcc
    69841 catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc cgttgacaag
    69901 gttgaagaga ttaaacagtc cgttgccccc gttcagccag gcatgtttgc tgatttcaat
    69961 accgacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg tacctttact
    70021 catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac caatgccgaa
    70081 agcataaata cttaacccgg cggtcatcca taaatacgcg tgcgatgaga taaccgttgc
    70141 cgcagcagcg accaacagac caatcataat cggccagccg cccataatta tcagcgaacg
    70201 tacggtgcgg cgcgaggtca aacgcgctaa caacaagtta cccgcaatta acgccccgaa
    70261 aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg taatgatgat
    70321 aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca gcgccagcgc
    70381 ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga gttctttcag
    70441 tgacagtttc tcgcctatac gcgtggcggt ttcaggcatg gcttgttgca gaccgaaaaa
    70501 ggagatcgct gccaatgcgg caaacaagac aaacatccct tcccagggca gcacatggat
    70561 ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt tcgccatcag
    70621 cgcggtgatc ttgatgcaaa ccgcctcttc gaaggattcc tgaattgcgg cgtatcccac
    70681 agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga attgttcaat
    70741 gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc ccgccagcat
    70801 caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta aaaacatccc
    70861 gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct gatattgttc
    70921 caccacggcc aacataccgg gttgaatcat atcgttgccg atataggttg aaaattcgta
    70981 aagcaccaga cagagaggga aaagtaacgc ctgacgtcca agcctggcac cggaagctaa
    71041 tttattttgc atgcaatttc ttcgccaata ataatcgcgc agagtttaat aaaagcacag
    71101 ctaacgagaa agcgaatttt gtagctgaaa ccacggttaa gcacattctt acattattgc
    71161 gagtatagct acgctttctt taagttttat ttaacctatg cccgttacaa ttacccaccg
    71221 taaacaggcc gcttgaggga aataagacga tgccgcttta cccagtttaa cctgcacttt
    71281 attctcaacg acttgcctgt attggctccc ttttaatcac tttgcgtcgg gaagttatgc
    71341 tggaaaattt gaatctctct ctattctctc ttattaacgc gacgccagac tcggctccgt
    71401 ggatgatctc gttggcgatt tttattgcta aagatttgat taccgtggta ccgctgctgg
    71461 ccgcggtgct ttggttgtgg gggcttacag cacaacggca actggtgata aaaatcgcta
    71521 tcgcgctggc ggtcagcctg tttgtgtcct ggacgatggg acatcttttt ccgcacgacc
    71581 gaccctttgt cgaaaatatc ggctataact tcctgcatca tgcggcggat gactcattcc
    71641 caagcgacca cggtacggtg attttcacct ttgcactggc atttttatgc tggcatcgcc
    71701 tgtggtccgg ctcactttta atggtgctgg ccgtcgtcat tgcctggtcg cgcgtttatc
    71761 ttggcgtgca ctggccgctg gatatgctcg gtggattgct ggcaggtatg attggctgcc
    71821 ttagcgctca gattatctgg caagcgatgg ggcataaact ctatcaacgt ctgcaatcgt
    71881 ggtatcgctt ctgttttgca ttaccgatcc gcaaaggctg ggtgcgtgac tgatctctgc
    71941 taaaaagtgt agtattgagc ggctcgcttc aataactatt cagagggatt atggaaacac
    72001 gtcgcgaaga gcgtatcggg cagctgctgc aagaattaaa acgcagcgat aagttacatc
    72061 ttaaagacgc cgccgccctg cttggggttt cggagatgac gattcgtcgc gatctgaaca
    72121 accacagtgc gcccgtcgtt ttgctcggcg gctatattgt tctggaaccg cgcagtgcca
    72181 gccattacct gttaagcgat caaaaatccc gcctggtgga agaaaaacgc cgggcggcaa
    72241 aaatggctgc gacactggta gaacccgatc agactctctt ttttgactgt ggcaccacca
    72301 cgccgtggat tattgaagcg attgataatg aaatcccttt taccgccgtt tgttattcgc
    72361 taaatacctt tctggcgctg aaagagaaac cccattgccg cgcatttctg tgcggtggtg
    72421 aatttcacgc cagcaacgcc attttcaaac ccatcgattt tcagcaaacg ttgaataatt
    72481 tttgcccgga tatcgctttt tattccgcgg cgggcgtgca tgtcagtaaa ggcgctacct
    72541 gttttaatct tgaagagttg ccggtaaaac actgggccat gtcgatggcg caaaagcatg
    72601 tgctggttgt cgaccacagt aaatttggca aggtgcgtcc ggcgcgcatg ggtgacctga
    72661 aacgctttga tattgtggtg agtgattgtt gcccggaaga tgagtatgtg aagtacgcgc
    72721 agacgcaccg tattaagttg atgtattaat gacgtataac cggatgacgt ttcgcgccat
    72781 ccggttatca gaagattaag agaaccagct gccgaaccac tgatggaatt tcatcatcac
    72841 gaaatcccac atccgaccaa agaatccgcc ctcttccaca ttttccatca cgatcagcgg
    72901 acgctgctca atggatttac cgttaagctg gaaatcaatg gtcccgacaa cctgaccttt
    72961 tttcagcggt gcggtaagct gcggttccgt taacgtgtag ctcgccttca ggtttttcag
    73021 ctgcccacgc ggtatggtca ctgagcccgc ttcgcctgcc ccaagattca cttcgctctt
    73081 atcaccaaac cagacgcgct gagtcacaaa ggtggcatca ggtttaattg gcgtcacggt
    73141 ttcaaagaag cggaaacccc aggtcaataa tttctcagac tcattaaaac ggatacggtc
    73201 agttttcgcc cccagcacta cggagattaa acgcatatcg ccctgggtag ccgaagcaac
    73261 cagattatat cccgcgcctg ccgtggttcc tgtcttcatg ccatcaacat tcagattgct
    73321 gctccacagc agacggttac ggttaggctg acgaattttg ttgaaggtga actctttctc
    73381 tttatgaatg gcgtactctt ccggcacatc gtggatcaat gctttaccca gcaatgccat
    73441 atcgcgcgcg gtactgaact gccccggcgc atccagaccg tgcaccgtct ggaaggtagt
    73501 gttggtcaga cccagttttt tggcataacc attcatcaaa ccaatgaatg actcctggct
    73561 cccggcaacg taatcggcca gcgcaataca ggcgtcatta ccggactgga taatcacacc
    73621 tttgttcaag tctgccaccg aaacctgatc gcccggtttg aggaacatta ccgatgaacc
    73681 acgcagtgcc ggatttcccg tcgcccaggc atctttaccg accgtcacca tatcggtgag
    73741 tttaatctta tcggccttaa gcgcctgccc aaccacatag ctggtcatga ttttagtcag
    73801 gctcgcggga tccagtttct catccgcgtt gccttctgcc agcactttac cgctggcgta
    73861 atccattaaa atccatgcac gcgcatccac gctcggcgct tcaacggttt gttccgccgc
    73921 gaatgccgtt ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac cacgaaggag
    73981 agaggagtat tgcgtcataa atgccacccg agtatccatt caaaaaaaat acgttatatc
    74041 gccgtatgat aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg
    74101 gtaaagtttt taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt
    74161 tgccagctgt tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagcctgtgg
    74221 ctgcctgtcg gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaaccaac
    74281 gtaaaaaaag tattgctgac gctcgaagaa ctggaactac cttatgagca aattctcgcg
    74341 ggccgtgagt ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg
    74401 gtgccgttgt tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc
    74461 cgctatctgg cggcacagta cgggcaaaaa cgtctgtgga tcgactcacc ggcacgtcgt
    74521 gcggaagcgg aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg
    74581 atcctgatgg gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc
    74641 agttgcaaag agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa
    74701 tggttctctg gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat
    74761 ttgtttaacg ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa
    74821 ctcactgaac gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg
    74881 ctaactttaa gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt
    74941 ccagtgcgaa catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc
    75001 actttgtcgc cgttgacgct catcacaatg acatctttat ctttcagcgc gccaataaat
    75061 aatttttgct gccactgggg gaatttatcg ctgttataga aggccatgcc gctcacagcg
    75121 ggcgaatctt tccagtaaaa aacaggttgc tcggtcccgg cgacgatctc ccctttcgct
    75181 tccggtatct taaagcctga atagttgatt ccccaggttg ccagcggcca gccgtagttt
    75241 ttgccttttt gcgggatatt aatttcatca ccaccgcgcg ggccatgttc attcagccac
    75301 agtgcattac tccacggatt catcgccatt ccttgcggat tacgaatgcc ataagaccag
    75361 atctcggcgc gcacaccgga ttcctttata aaaggattat catccgggat ttcgccctgg
    75421 tcggtcagac gcaccagttt gccctgtaat ttatccagat cctgcgccgt cgggcgctga
    75481 ttgttttcgc ccagagcaat aaaaagataa cctttaccgt cgaataccag ccgcccgcca
    75541 aaatggttgc cggtagacag ttttggcatc tggcggaaga cggtgcggaa gtcggtcact
    75601 tttgagagat catcacttaa gcggccataa cccacagcag ttccggcttt gccatcatcg
    75661 ccaacttcgg aataacttaa ccagatgcgg cgagactgag caaaatcagg cgctaaaacc
    75721 acgtccagca ggccgccctg cccgtgcgcc caaacgtccg gaactccgga aagcggcgca
    75781 gataatcctt ttcctgcttg ccagtgacgc aactcgccgc cgcgcagagt gattaacata
    75841 ccgtgattat cgggtaaaaa ggccagtgcc cagggatggt cgagtttgtc ttgcagtact
    75901 tcgacattta ccgttgcagg agccgcccag agagcggaag aaagacaaat aaggggcaca
    75961 aggaaaaagg attgtcgatg cataacgcct ccggattgac tctggcttaa agcgtagtca
    76021 gtggaggaga taacaaattc atttttacaa aaacttaaac atgaaggggg agacgctttc
    76081 tcccccttag ttttcaggcc ttctcaagca tggcgtgctt ctgcaggctc tggatactca
    76141 gcgttaagct catcagacaa ttttcaagct tatcggcgtt gacggtaata acagtcgggc
    76201 aatcatggtg cccactcatc aaacatactg cggctgtcgc taatgcttct tcagcatgat
    76261 gaagagcact ccactcttcc tgatccagat gaagattcaa ccgcagcgat ttatcgtgca
    76321 gttcgcgatt cagtttaaaa aagttatctc gtagatgatt gctttcgctg acggacatgt
    76381 atccttttgc cttcctcagt tgcacgtatg cagcgaggtc aacgcgtgcg tcgatcaacc
    76441 ggttcagcag atcgattcgc tgtctgtcaa cgaacatact tcgatcctcc tctttccagc
    76501 cttgttgcaa ttaaatagta tggctatttt ttgagcaaaa tgctgtccag gatcaattct
    76561 tcacgccgaa ttaacaaaat ttacatttcc ttcgcctccg ccagcgaatg agattgcgat
    76621 acaaatttta tccagaactc tgtagaatcc ctgccctgac ctttcctgta actgaatatt
    76681 ttttaagcta tgagcaaagt aactccccag ccgaaaatcg gctttgtttc ccttggctgt
    76741 ccgaaaaacc ttgtcgattc agagcgtatt ctcaccgaac tacgcactga aggttatgac
    76801 gtggtaccga gctatgacga tgcggacatg gtgatcgtca acacctgcgg ctttattgac
    76861 agcgcggtac aagaatcact ggaagccatt ggtgaagcgt tgaatgaaaa cggcaaggta
    76921 attgtgaccg gttgtctggg ggcaaaagaa gatcagatcc gcgaagtcca cccgaaagtg
    76981 ctggaaatca ccgggcctca tagctatgag caggttctgg agcacgttca tcactacgtg
    77041 ccaaaaccga aacacaaccc attcctgagc ctggtgccag aacaaggtgt gaaactgacg
    77101 ccgcgtcatt atgcctatct gaaaatttcg gaaggctgta atcaccgctg caccttctgc
    77161 attattccgt ctatgcgcgg cgacctggtg agccgtccga ttggcgaagt gttaagtgaa
    77221 gcgaaacgtc tggtagatgc gggcgttaaa gagattctgg tgatctcgca ggatacttcc
    77281 gcctatggcg ttgatgttaa acatcgtact ggcttccaca acggcgagcc ggtaaaaacc
    77341 agcatggtca gcctgtgcga acagttatcg aaactgggga tctggacacg tctgcactac
    77401 gtttaccctt atccgcatgt ggacgacgtc atcccactga tggcagaagg caaaatcctg
    77461 ccgtatctgg acattccgtt gcagcacgcc agcccgcgca ttctcaaact gatgaagcgt
    77521 ccgggttctg tagatcgcca actggcgcgc atcaaacagt ggcgcgaaat ctgcccggaa
    77581 ctgaccctac gctcaacctt tattgtcggc ttccctggcg agacagaaga agatttccag
    77641 atgctactcg acttcctgaa agaagcgcgt ctggatcgcg ttggctgctt taaatacagc
    77701 ccggttgaag gtgcagacgc caatgccctg cctgaccagg ttccggaaga agttaaagaa
    77761 gaacgctgga accgtttcat gcagctgcag cagcagattt ccgccgagcg cctgcaagag
    77821 aaagtgggcc gtgaaattct ggtgattatc gacgaagtgg acgaagaagg cgcgattggt
    77881 cgcagcatgg cagatgcacc ggaaatcgac ggcgcggttt atctcaatgg tgaaaccaac
    77941 gttaagccgg gtgatatcct gcgtgtgaaa gtcgagcacg ccgatgagta cgatttgtgg
    78001 ggtagccggg tttaattaat tcaggccggg caaaatcatt tgtccggcct gcgaataatg
    78061 attactgcca gctaatctga gccctgccgt ttttcttact cttatacagc cgctcatccg
    78121 ccagcatttt caagcgttcg acggtggtac attcatttga atgcgctaca ccgatactgg
    78181 tacttaaatt aatgcttccc acctggaaat gaacgatttc ttttagaatc gatagcaact
    78241 cttccagcgt tccacccgta taaatcaccg caaattcgtc accgccgaag cggtagattt
    78301 tacctttatc taccgggatc tgtgatttaa ggattttcac cactttcatc aacacttcat
    78361 cgcccagcaa atgaccgaag gtgtcgttaa tgtttttgaa tttatcgacg tcaaacaggc
    78421 acaaataacc gcctttttcc ggcagcgctt ttaaatcttc gtcaaaggat tttctgccca
    78481 ttgctttggt caggtcgtcc aggaacgccg agttatacat actgtagagt tttttgacat
    78541 cggcagccga aaagaaaagc ccgccatcca gtacgcgttc aaaatggtta ttaatgatgg
    78601 cttctttctc cacaatacca cgattaatca ctctgcgtac cagatagagg caactggtac
    78661 ccgatatcac tgccaccaat gcggcaaaga aaataatcaa tgtacagacc cgcaaataaa
    78721 catctttttt atacgcaaat acgtacaatc cggtcggcaa ataggcgaag tgctccatcg
    78781 cttccgaaac ggtgaagatg gcgtttttag cataatattc atcaccggtc accggtttac
    78841 cgttcttaaa tagagagaac atcaggttct cgctgtccat cgccagcgat tttgacagta
    78901 aagaaaaacg ctccagacca agcaaaaata tcagactacg attgtgagtt accggataga
    78961 ggtaatagac gaaggtttta ccgtcaaaag agataaaccc tgtatttttg acatgtcgat
    79021 acttcacaac ctgcataata tctgcacaat aatttttctc gaactccttt acctgaatac
    79081 attcgtcact ggaaatcaga taaggaccga catcgtacaa ataagagggg gaaaccgagt
    79141 cggcgtaagc tgtgcgcaaa ccaaccaaca gctgttcctc cgtgggattt agcgtgttga
    79201 taagatcaat tgccacgcta cgccgatatt cagcaatatt tgccaccgca aaagcagtat
    79261 caataacgga ctgtttcagg ctatacaccc gttcctttac catttgagtg tagatcccgc
    79321 aggcaactgc tgaaatcatg ataaaaatca gcagactgac tgtaagtacg aacttattga
    79381 ttctggacat acgtaaacta ctcttttact aattttccac ttttatccca ggcggagaat
    79441 ggcatgggtt tctggtacag atacccctga gcctgaacac atcctaattt aattaattcg
    79501 cgcgcaacgt cttcgctctc tacgccttcc gcaatcaccg ggatccggtt atagcgtgcc
    79561 agatcgaaaa taatgcgcac aatttttccc gcagtaccga aaatatcgcc ggtaaattgc
    79621 gagtccacct ttatgctgtc tggattaatt tcataaaaac gtttcaggtt tgacaaaccg
    79681 gtgccaaagt catcaatcga tatccgtacc ccttgtttac gcagttgttc aatattgaga
    79741 taaaaatgtt cattaattcg ctcgatcacg gtttcagtga tctcaacaca gaggctgttc
    79801 gccttcaggc cattttcttt catgatggtg gtaaaacgct catgaaagtg cggttcgttt
    79861 aactgccagg gcgaaacatt aatatgcagt aagaaatcat ccttcagcga cgcacgctga
    79921 ctaaaatgtc tgaattcacc cattgccgtt ttaataatct gataacccag ctcattgata
    79981 aacccgatat cttcagcaat ggtaatgaac ttcagcggtg aaatgatccc ccgctccgca
    80041 gaaacccaac ggcacaacgc ctccgcgccc agaatggcgc gggtgttaat atcaacgatc
    80101 ggttgataga cgaggaatag ttcgccaatt tccattgcat ggcgaatatt acgcgcaatc
    80161 cagatattct cttctatttc gttgagtttc gcgtcatcac agaaaataaa ctcggagaac
    80221 atatggtcac ggatactgga aatcgccagt ttcagttttt ctgcgtaact ccaggcgttt
    80281 tccccctgca aaggttcaca aatggcagac ttatggatgc gcagattgcg gtagccaaag
    80341 ctgctgtcaa aatcgtttac catcgctatt ttacgggtaa atgtctgtac attttcccga
    80401 cacagaagta aatagagatc ggcattgaat gtcacgatat cgcagcattg cgcatatttc
    80461 tcggcaattc gctgacacaa tttcctgacg attttattgc gttcatcaac accaaacacc
    80521 gcttccacgg cttccacggc ttccagacca cacagtttga tgatccccac agccagatac
    80581 tgattattgt tggactcaat aatcgccata tttttaataa aaccgctttc ctgatcgtaa
    80641 gagatcagtg agagcaattt atttgaccgt tcaatttcgg catctttcat ttccgcgatc
    80701 gaatttttga tgctgataat ctctttcgca tcgccttcgc gaattttcag agcctcgatc
    80761 tgatccgttg agtcgggcaa aaacgccagc gtattaacca gctcggtcat ttcagaaaat
    80821 tgccgacgga acacacgagt ggtcatgaaa tataccacca gcaacacgat aataatgaac
    80881 gcaaacggta gataaaaaag tttatctgat gcgttcaata aattaccgcc aggataataa
    80941 aaatagattt cgaacaatgc attatttggc actgctgatg tctttgcaat atactccgta
    81001 tcgacagaat caataatatt ataattacct tcgctattat aaataatcag tttttcacga
    81061 gcgagttgat tcttggcaat gatctcatga ccttgtttca aaaatagaac ttcatcatta
    81121 gcgctgattt gatcaagata ctcgttcaga taccgccagt ttaaatcgac atgaatttta
    81181 tctaccgcta acaaatcgtg atctttgccg ttgatacgta gcgtaaaaga gatacatttt
    81241 ttacccggac gatcaaagca attgtatgcc ggattccagc tgttgttaga gtcatctgaa
    81301 gccaccgacc acggtcgttt cgatgggtta aaatcggcaa aaataacccg ccctgactcg
    81361 tcgacaagcg gtccatttac ttgttcctga tgataaacaa cgattttatc attcgccctc
    81421 cgtgaaaaga gataatattt attatcatca agtaccagtc ccaccgagtc taaaaatggc
    81481 atcatttcca ttttcagaat cacggcgcgt tgaatctcct ctgtattttt taataaatac
    81541 agtgagtcct ttaatgtcag ggccgaacgt tcaatatctt taaagaaata gccaacgttt
    81601 ttctgaataa gttcaaagcg gctatcggca cgttttaccg cgcgttccga taccagttga
    81661 tagttaatga tgaaaaaaag accaataaat gacagtgctg ccaataaaaa taaaattatc
    81721 agccttatct ttatcttttc gtataaactc agcatgttaa tacatttaac aaaataacta
    81781 tctgaacgct gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac caaattaccc
    81841 gattctacaa aatcagcaga aagttacaac acttgcttag ggcaataatt tcctgtgagg
    81901 tgattaccct ttcaagcaat attcaaacgt aactatcctt taattttcgg atccagcgca
    81961 tcgcgtaaac catcgcccaa caaattgaac gccagtacgg tcagaaaaat agccagggcc
    82021 ggaaaaacag cgacatgcgg cgcgataacc atatccgctc gtgcctcatt gagcattgct
    82081 ccccactctg gtgtcggcga ctgcgcaccg aggccgagaa atgacaggct ggcggcagag
    82141 ataatcgagg tgccaatgcg catggtgaaa aacaccacga tagacgagac ggtcccaggc
    82201 aagatatgac gcaatgaaat ggtcatatcg ctggcaccaa tactgcgtgc tgactcaata
    82261 aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca gtcgggcaaa cgcggggatg
    82321 gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc ttcccaacac cgcaacaaca
    82381 gcgatcgcca gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg catgatcaac
    82441 cgatcccacc agccttcata atatccagcc aacaagccca gcaacgtccc gatcgccgca
    82501 ccgataaaca cggcaaacac gcccgccgcc agcgagattt gcgcaccaac caggacacgg
    82561 ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga aggtccgtta
    82621 ttcagattgt cataatcaaa ataattttcg gcgtcatagg gagcgatcca gcgtgcaaaa
    82681 atggccacca caatcaataa aataacgaat aatgcggcgg tcatcgccat atgctgacgg
    82741 cgaaatcgtc gccagaattc atgccacggt gtacgcacct ggtcaggttt gaccagtggc
    82801 atggcgttta acaccgcctg acgtcgccag ttaaatagtc gcatccttac ttgtacctga
    82861 tagccgggtt aatggcggcg taaagcacat ccaccactaa gttgataaga ataaattcca
    82921 gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc gcgcatttct acggagtcaa
    82981 cgagtaagcg tccaagtccg ggccagttga aaactttctc caccacgata gaaccaccga
    83041 gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg gatcatcgcg ttacgtagcc
    83101 cgtgtttgag gacaaccctg gtttcgctca cccctttcgc cctcgcggta cgcatataat
    83161 cttcgcttaa aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg gctgccactg
    83221 ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca actgtctgct cccacggtag
    83281 gcagccagcc cagttcaacg gagaatacct gaattaaaag catccccagc gcaaatgccg
    83341 gaaacgagat ccccgacacc gcaatggtca tactcaatcg atccggccaa cgattacgcc
    83401 agacggcggc gataattccc gccgccatac caaatatcac cgcccagacc atactggtta
    83461 tggtcagcca cagcgttggc ataaagcggc tggcaatctc atcggcaacc ggacgacgcg
    83521 acaccatcga caggccaaaa tccccctgca cagcattgct gatatagtgc cagaactggt
    83581 gatacagcgg ctgatccaac cccagctgct gacgcaccag ttctataacc tgcgcatcag
    83641 cttcgggccc ggcaatcaat cgcgccggat cgccgggcag catatgcaca aataaaaaca
    83701 ccagcaccga gacgataaac agcgtcggaa tcaaccccag taagcgtttg ataacgtaat
    83761 taagcattcc actccctgcg ttgcttattg caaatccgcg tcttcaaagc tgaagccggt
    83821 gtctggcatg atccaaaaac cggtcaggtt tttactgtgt gccgacacca gtttttctac
    83881 caccagcggg atccacggcg actcttgcca gatgatatcc tgcgccgcct tatacaagcg
    83941 ggtcttttcc gccggatcat tagttttcag tgcctgagcc aggaagtcgt ccacctgttt
    84001 attgctgtaa aatgcggtat taaacagcgt cggtggccag ttttgcgagg cgaacagtgg
    84061 cgatagcgcc cagtccgctt cgccggttga agccgaccag ccagtgtaga acatccgcac
    84121 gccgctctct ttttgccctt taccttcaac ttctgccgcc cgctgtccgg catccatcgc
    84181 ggtcacctgg gctttaatcc cgacctgcgc taactgctgc tgggtaaatt gcagcacttt
    84241 ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt tgggatatcc
    84301 cgcctctttc agtaattcgc gcgctttcac tggatcgtaa ggccacggtt tataactttg
    84361 cgcgtaggcg atacttggcg gtaccacacc agtagctggc gttgcatagc ccgcaaaggc
    84421 aactttcacc agcgccggac ggttaatggc gtaattcagc gcctcacgga ccttcgggtt
    84481 atcgaacggc ttttgcgtca cgttcatact gatataacgc tgcataattg acggactggc
    84541 catcaactcg atatttttgt ttttctccag cagtgtggcc tgctcgtagg gaatggggaa
    84601 agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat ccgccaccgg
    84661 acgccaggtt atgctgtcca gtttgggcaa tcctggctgc cagtaacccg cgaatttttt
    84721 caccttcaca aaatcggtct gattccaggt atccagttca tacggtccgg ttcccaccgg
    84781 atgaaaacca atctccttgc catatttttc cagcgctgcc ggtgaaatca tcgcggtcgc
    84841 cggatgggca agaatattaa taaacgctga gaacggctgt ttgagggtaa tctttaccgt
    84901 tgtcggatcg atcgcctccg ttttggcaat attcttatac aggttatagc gtttaagatg
    84961 attcgccgga tcgctggccc ggtccagatt cgcttttacc gccactgcgt tgaaatcggt
    85021 gccatcctgg aatttaattc cttcccgcaa tttcacgatg taagtaatgc cgtcatcgga
    85081 aacggtataa ctctccgcca gcacgttttt cagtttcatc tctttatcca gaccgaacag
    85141 cccctggtaa aacgatttcg ctacggcctg agataacgtg tcatttgcgt catacggatc
    85201 gagcgtggtg aaattcgatc ctaccgccac caccacatct ttggcagcga atgcacaaga
    85261 tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta cagctcttgc
    85321 cattttattc tccgcctgaa tgttatctac gcatgaatgc gtattctgat tgtggttgtg
    85381 cgacgtaatg ccccggcccg acgcattgca acgagacggc tgccacctct tcgccacgca
    85441 gatgaatatt gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt tgtcgggacg
    85501 gttcagcgac cggaactgcc gccagtaatt tacgcgtata aggatgctgc gggttttcga
    85561 agaccgcgcg ccgtggacca atttcaacaa tttgcccgag atacatcacc gccacacgat
    85621 gactaatccg ctctaccacg gccatatcgt gggagataaa cagatacgca atgccgaaat
    85681 cacgctggag atcgagcaac aagttgataa tctgcccgcg aatagaaaca tccagcgccg
    85741 aaacggcttc gtcggcaatg atcacttttg gattcaatgc caacgcgcga gcaatgcaga
    85801 tgcgctggcg ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt tcaggtaaca
    85861 ggcccacgcg ctccagcaac cacgcaacgc gtgcagccgc gtctttacct ggcaataaac
    85921 cgtgtacacg cagcggttcg ataatcgaat caccgatggt ctgacgtggg tccagcgaag
    85981 cgtaagggtc ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt ttgccgggtg
    86041 acaaggtatc gattctctga ccgttaaaga taatttcgcc gccctgcgat tcgaccaggc
    86101 gcagcaacgc ccgcccggta gtggatttac cgctgccaga ctcgcccacc agcgatagcg
    86161 tttcgccagg ccagagatca aaactgactt tctcaacggc atgcacttcc cgcgttacgc
    86221 gattcaacaa accgctgcgc aaagggaaac gggtgacaag attacgcact cgtaaaacag
    86281 gttcgccatc caccaccgtt ttctgctcga tggggggggc ctgtttcgct ggatgttcaa
    86341 gcgatatcaa cgggaaacgt cggggataat ctaacccttt catcgcacca agttgcggaa
    86401 cagcagctaa cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc tgttcgacgg
    86461 tacccgtttc caccgcctcg ccctgataca tcaccagtac ccgatcggca atctctgcca
    86521 ccacgcccat atcgtgagtg ataaagataa cgcccatcga catctctttt tgcaatactt
    86581 tgattaattg caggatctgc gcctgaatag tgacatccag cgcggtggtt ggctcatcgg
    86641 caatcagcac cgccgggcgg catgacagcg ccatcgcaat catcactcgc tggcgcatcc
    86701 cgccagagag ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga atgcgtacct
    86761 gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc tgatgcagac
    86821 gaattgattc ggcaatctgt tcacccacag taaataccgg gttcagcgat gtcatcggct
    86881 cctgaaatat catcgccata tccgcaccgc gaacatggcg catttgtgca gcgctctgct
    86941 cgctaagttc aatcacttcg cgactgcgcc gctgcaacag cattttatcg cactgtacta
    87001 aaccgcccgc ctgttccaac aggcgcatca atgccaacgc agtcactgac ttaccggagc
    87061 cggattcgcc aacaattgcc agcgtctcac cgcgttgcag actaaaagag agattgcgga
    87121 ccgcagctat tttctgctgg tcctgcataa aggcaatatt cagattttca accgccagca
    87181 cattaccggc atcaagttca tcactgtgtg gcaacggtgt cccctttttc acggtagata
    87241 ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat acattccttc ggtgttaaac
    87301 ggtagcgcga cattcccttc atggtcgata gcgattaagc caccgctacc gccaagcgca
    87361 gggagttttt ccattactac ccgctcgcag gcttccgcga gacttaatcc gccgtaatcc
    87421 attaacgcgg cgatgtcata tgccgccagc gcgcggatga agacttcgcc cgtgccggta
    87481 caagaaaccg ccacactggc gttattggcg tagcatccgg cacccactaa gggactatcg
    87541 ccaactcgtc cgggtaattt attggtcatt ccgcctgtgg acgtggctgc cgccaaattg
    87601 ccgtctaaat ccaacgccac ggcccccacg gtgcccattt tctgtttttc atccagtggc
    87661 gcaccgctat ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag ttgttcataa
    87721 cgcaaaggcg tggagaaaat ctccggcgag acgcgctcca tgccacgagc aaacgcaaaa
    87781 ttttctgccc cttcgccaat catcatcaca tgcgggcttt gctccatcac cagccgggcg
    87841 gcaagaaccg gattacgcag atgactaacg cccgccaccg caccggcttt cagggtgtta
    87901 ccatccatca cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac agcgccaatt
    87961 ccggcgttaa acagtggaca ctcttccagc agacgcaccg cttccgtcac cacatccagc
    88021 gcactttcgc ccgcttccag cattttctgc ccggtttcaa caatggcaga caacgcctca
    88081 atgtagcgta attcctgttg cagactcatc tgcgcgcggc taattgcacc tgcgccacca
    88141 tgaattgcaa tgactgcttt gcccatagta ttcgtccatt aagggtggct cgccactttt
    88201 gctataaata tcagaatttt tatgaattac tcattcaatt tttgaatata gaaagatgga
    88261 tatggcatgt aaaggcagga gccaacgcta cgcccgaggt ggcatacagt ctgtggcgtt
    88321 tttctgacat aataggcaaa ttcgattttg cctccgcagg agtgttttca tggaatttac
    88381 caccggattg atgtcgctcg acaccgcgct taatgagata ctttctcgcg tcaccccact
    88441 gaccgcccag gaaacgctgc cactggtaca gtgttttggt cgtattctgg cgagcgatgt
    88501 cgtttcgccg cttgatgttc cggggtttga taactccgca atggacggct acgcggtgcg
    88561 tttagccgat attgcctcca ggcaaccgct gcccgttgcc ggtaaatcct ttgccggtca
    88621 gccgtatcat ggtgaatggc ctgcgggcac ctgcattcgt attatgaccg gtgcgccggt
    88681 gccggaaggc tgcgaagcgg tggtgatgca ggagcagact gaacaaacgg acaatggcgt
    88741 gcgttttact gctgaagtgc gtagcgggca aaatattcgc cgtcgcggtg aagatatctc
    88801 tgcaggtgcg gttgttttcc cggcgggaac tcgcctgact accgcagagc tgccagtaat
    88861 tgcttcactg gggattgccg aagttccggt gattcgtaaa gtgcgtgtag cgcttttttc
    88921 taccggtgat gaactccagt tgcccggtca gccgctgggc gacggccaaa tctacgacac
    88981 caaccgtctc gccgtacacc tgatgttaga acagctgggt tgcgaggtaa tcaacttagg
    89041 gattatccgc gacgatcccc atgccctgcg cgccgcattt attgaagccg acagccaggc
    89101 ggatgtagtg atcagttccg gcggtgtttc agtgggtgaa gcggattaca ccaaaacgat
    89161 tcttgaagag ctgggggaga tcgccttctg gaagctggcg attaaaccag gtaaaccgtt
    89221 cgcgttcggt aaactcagca atagctggtt ctgcggcctg ccgggcaacc cggtttcagc
    89281 gacgctgacc ttctatcaac tggtacagcc tttgctggca aaactaagcg gcaacaccgc
    89341 cagcggcctg cccgcgcgcc agcgcgtacg cacagcgtcc cgcctgaaaa aaacgccagg
    89401 acgtcttgat ttccagcgcg gcgtgctgca acgcaacgcc gacggtgaac ttgaagtgac
    89461 gaccaccgga catcagggtt cacatatatt tagctccttt agtctcggca actgctttat
    89521 cgtgctggaa cgcgatcgcg gcaatgtgga agtgggcgaa tgggtggaag tagaaccgtt
    89581 taacgcgttg ttcggaggcc tgtaatgacg gaactcagcg atcaggagat gctgcgctac
    89641 aaccggcaaa tcattctgcg cggttttgat tttgacggtc aggaggcgct aaaagattct
    89701 cgtgtgctgg tagtcggcct cggtggactc ggctgcgcag cttcgcagta tctggcaagc
    89761 gccggtgtcg gcaacctgac gctgctcgac ttcgactcgg tttcgctctc gaatctgcaa
    89821 cgccagacgc tgcacagtga tgctacggtc gggcaaccga aggtagaatc cgcccgtgac
    89881 gccatgacgc ggatcaaccc acatatcgcg attacgccag tcaatgcact gcttgatgac
    89941 gctgaacttg ctgcgatgat tgcaaaacac gatctggtac tcgactgtac ggataacgtt
    90001 gcggtgcgta atcaactgaa cgctggctgt tttgccgcga aggtgccgct ggtttccggc
    90061 gcggcaattc gtatggaagg gcaaatcacg gtcttcactt atcaggacgg cgaaccgtgc
    90121 tatcgctgcc ttagccgttt gtttggcgag aacgcattaa cctgcgtaga agcaggcgtg
    90181 atggcaccgt tgatcggcgt aattggttcg ttgcaggcaa tggaagcgat caaactgctg
    90241 gcaggttatg gcaaaccggc cagcgggaaa atcgtcatgt acgatgcgat gacctgtcag
    90301 tttcgcgaaa tgaaactgat gcgcaatccg gggtgtgagg tgtgcgggca gtaattgcca
    90361 cctccaggcc agataagacg cggcactccg ccacgtcatc agcaatttat gaggatgcag
    90421 aacgtccggt taaatcgacg ttctgccaaa cgctcccagc cagtcctgtt caaacttcgc
    90481 cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca gtggcagcgt
    90541 aattgattca catcctgcca gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc
    90601 tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt cggtcacagt
    90661 ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa catattccgc
    90721 acctgccagc gccgacagca gcccttgtgc tgcgccatat accgcggttc ccagcgttgg
    90781 aatcccttcc gcttttaaca tcttaatagc tgccagcccc tcggcggtca ccggaacttt
    90841 caccacgata tccgcaataa tagaacgcaa tttacgtgcg tcattcacca tcccttcagc
    90901 agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt catgaagttg
    90961 cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt tagtggtcac
    91021 acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg aagtatccag
    91081 atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac tatcacggga
    91141 aaactctctg aacagttgac gcacatcaat ctaactttca ttcgaaagta ttttaatgtt
    91201 tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga cccatgatgg
    91261 ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc gctggtgtca
    91321 gaacccggaa agccgcgccc gcacgcagga tctgctgtat gacgcacgac tgtgtctgga
    91381 aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc tgaatggttt
    91441 gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg actgctgtcc
    91501 gacacaggca ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca tgacgaccgt
    91561 tctgcgcgat aaaccgtttt atgatcgcag cggcggcggt ttaacgcttt cgggtggtga
    91621 gccctttatg cagccggaaa tggcgatggc gctactgcaa gccagccacg aggcaggcat
    91681 tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc cttctctgcc
    91741 ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg
    91801 gaccgacggt aacgccgcca gagtgctgga taacctgaaa aaactcgccg cagcgggcaa
    91861 aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa cctctgtaaa
    91921 agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt ttctgcccta
    91981 ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg ctccggaaaa
    92041 accgcttgat gcgccagaac tgctcgactt tgcccagcag tatgcctgcc agaaagggtt
    92101 aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac gctcagcgac
    92161 cgcattaaag cgcacaaaaa tgcgctggtg catattgtga aaccgccagt ctgtaccgag
    92221 cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat cccggtacgt
    92281 cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa acacgatgag
    92341 ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc ggaatatact
    92401 gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc tggctttgcg
    92461 gtgagcgaag agaacaaacg cgttctgcat gaagtgtgcc cgtggtggcg cggtcagacc
    92521 gtacaggatc gctgctacgg catgtttacc gatgagcaaa aaggtctgct ggcgaccgga
    92581 atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt gaatttcccg
    92641 ctgctgctgg aaaaaggtct tgatggtctg cgtgagaaag tagcggaacg tcgctcgcgc
    92701 atcaatctga cggtgctgga agatttacat ggcgaacaat tcctgaaagc gattgatatc
    92761 gtgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg tgaaatggct
    92821 gctaccgaaa ctcgcgaaag ccgtcgcgat gaactgctga cgatggcaga aaactgcgat
    92881 cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg ttacttcatc
    92941 caattgattt tgcagatcga atctaacggt cactcggtat cgtttggtcg tatggaccag
    93001 tatctctacc cgtactatcg ccgcgacgtt gaactcaacc agacgctgga tcgcgaacac
    93061 gccatcgaga tgctgcatag ctgctggctg aaactgctgg aagtgaacaa gatccgctcc
    93121 ggctcacact caaaagcctc tgcgggaagt ccgctgtatc agaacgttac cattggcgga
    93181 caaaatctgg ttgatggtca accaatggat gcagtgaatc ccctctctta cgcgatcctc
    93241 gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta ccatgcagga
    93301 atgagtaatg atttcctcga cgcctgcgta caggtgatcc gttgcggctt cgggatgccg
    93361 gcgttcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat tgaaccgcag
    93421 gacgcttacg actacgcagc gattggttgt atcgaaaccg ccgtcggtgg caaatggggc
    93481 tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa
    93541 ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc gttgtcggca
    93601 ggcaacttta acaatttcga tgaagtaatg gacgcgtggg atacgcaaat ccgttattac
    93661 acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga gaacgtgcac
    93721 gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag tatcaagcaa
    93781 ggcggcgcga aatatgactg ggtttctggc ctgcaggttg gcattgccaa cctcggcaac
    93841 agcctggcgg cagtgaagaa actggtattt gaacagggcg cgattggtca gcaacagctg
    93901 gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg tcagcggctg
    93961 attaacggtg cgccgaagta cggcaacgac gatgatactg tcgatacgct gctggctcgc
    94021 gcttatcaga cctatatcga cgaactgaaa cagtaccata atccgcgcta cggtcgtggt
    94081 ccggttggcg gcaactatta cgcgggtacg tcatctatct ccgctaacgt accgtttggc
    94141 gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc agaaggcgct
    94201 agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc ggtgggtaaa
    94261 ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa tccggcaacg
    94321 ctggagaacg aatctgacaa gcagaagctg atgatcctgc tgcgcacctt cttcgaggtg
    94381 cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct ggaggcgaaa
    94441 aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc cgccttcttc
    94501 accgcgctct ctccagacgc tcaggacgat atcatcgctc gtactgaaca tatgctgtaa
    94561 tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca atttcatcct
    94621 tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac tgttacggtt
    94681 tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag acatggacgg
    94741 tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc aatatcagga
    94801 actgaaaaag cgcggcattg agttcgttgt cgccagcggt aaccagtatt accagcttat
    94861 ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg gcgcactggt
    94921 ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat cgcggattgt
    94981 tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc tgcaaagtgc
    95041 atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac actaccatcg
    95101 cctgaaagct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt tttcgctcaa
    95161 cctgccagat gaacaaatcc cgttagtgat cgacaaactg cacatagcgc tcgatggcat
    95221 tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg gtctacataa
    95281 agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa atgtggtagc
    95341 gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt cctttgcgat
    95401 gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg ataataatca
    95461 tgaaggcgcg ctgaatgtga ttcaggcggt gctggataac acatcccctt ttaacagctg
    95521 acctctctac cggagccgtg cgctccggtt ttcccttctt tttacatcct gtacatcaca
    95581 tcaaatcatt aaacttgcat taacttattt ataacttata ttgtcacatg caaatcagaa
    95641 tggtttcgaa tgaaagttaa tgagaggtgg ttatggcttc tacttttacc agcgacacat
    95701 tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg
    95761 gcgacgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga gtggctgaaa
    95821 tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat gccgatatca
    95881 aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga
    95941 taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg ctggactatc
    96001 tggaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc ttcgggacgc
    96061 tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa atgcaggccc
    96121 gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa
    96181 gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac cgtatccgcc
    96241 gccgtccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc ggggcgctgg
    96301 aacgctggct gcttccagcg tatcagcgcg ttttcgccaa cgtctttaat ggcaatctgg
    96361 cgcaatatga tccctggcat gcggcacatc gtacggaagt tgaagagtac acggtggaca
    96421 acaccaccaa atgttccgtg tttcggacat tccagggctg gacagcgctc tctgatatgc
    96481 tgcctggtca ggggctgctg cacgttgtgc ccattcctga agccatggcg tacgtactgt
    96541 tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg cccggaagag
    96601 tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc agcattccaa
    96661 aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc gttgcccccg
    96721 ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga
    96781 aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg
    96841 acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt gccgacctca
    96901 acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa gtgaattgat
    96961 gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg ctttcttaat
    97021 gcctgatgcg ctacgcttat ctggcctaca aagtcctgca gattcaatat attacaggta
    97081 ctgcgtagat cctgataagc gtagcgcatc aggctatttt gcatttgtca tcagccgccg
    97141 tcaccccgac agtatcgggg tgacggataa tcacttactc gatcccttta ctacgcaggt
    97201 aatcttcgta attaccgcta aagtcgatca cgcgttccgg ggtgatttcc agaatgcggg
    97261 tcgccaggga gcttacgaac tcacggtcgt gtgaaacaaa gatcagcgtg ccctgataca
    97321 gttccagcgc catgttcagt gactcgatgg attccatatc caggtggttg gtcggttcgt
    97381 ccatgatcag aatgttcggc ttctgcatca ttaacttacc aaacagcatc cgcccttttt
    97441 ccccaccgga aagcacttta gctggctttt tgatgtcgtc ctggctgaac agcaaacgac
    97501 cgagaatgct gcgtaccgcc tgctcgtcat cgccttcctg cttccactga ctcatccatt
    97561 cgaacacggt cagatcattt tcaaactcat attcgtggtc ctgggcgtag taaccaatgc
    97621 gcgcgttttc agaccatttt acggtgccgc tgtccggttg cagatcgccc accagcgttt
    97681 tcagcagcgt tgatttaccg acgccgttgg tacccagtac cgcaagtttt tcaccgacct
    97741 ccagcagcag gttgagattt ttaaacagtg gaccgttatc aaaccctttg gtcagacctt
    97801 ccacttccag cgcgttacgg aacagtttct tatcctgttc aaaacggatg aacgggttct
    97861 gacggctgga ggctttcacc tcttccagtt tgattttatc aatctggcgc gcgcgcgaag
    97921 ttgcctggcg agatttcgag gcgttggcgc taaagcggct aacgaaagat tgcaactcag
    97981 caatctgcgc tttcttcttg gcgttatcgg ccagcagacg ttcacgcgcc tgggtcgccg
    98041 ccgtcatgta ctcatcgtag ttacccggat aaacgcgcag ctcgccgtaa tccagatccg
    98101 ccatgtgggt acagaccatg ttaaggaagt gacggtcgtg cgagatgatg atcatggtgc
    98161 tgtcacgctc gttcagcacc tgttccagcc agcgaatggt gtcgatgtcg aggttgttgg
    98221 tcggttcgtc gagcaggaga atatccggat cagcaaacag cgcctgcgcc agaagcacac
    98281 gcagcttcca gccaggagca acttcactca tcgggccgta gtgctgttcc actggaattc
    98341 ccacgccaag caacaattca ccggcgcgag cttccgcaga gtaaccgtcc atttcgccgt
    98401 atttaacttc cagatcggcc actttatagc cgtcttcctc actcatttcc ggcaaagcat
    98461 agatgcggtc gcgctcctgc ttcacttccc acaactcttt atgccccatg atcaccgtat
    98521 ccagcacagt gaactcttca aaggcaaact gatcctgacg cagcttacca atgcgctcgt
    98581 tgggatcgag ggaaacgtta cccaaagtcg gctctaagtc gccgccgagg atcttcataa
    98641 aggtggattt accactaccg ttcgcgccaa tcaggccgta acggttgccg ccgccaaatt
    98701 tgacggaaat gttttcaaac aacggcttac tgccgaactg catggtgacg ttactggaaa
    98761 ctaacacagg cgtatcctga aaagagatat gacaaaccgc gcattatgcc ataagtctga
    98821 aagaagatcc tgctcctcgc ttaaggtcta cactcaagcc aaaggcaaaa aatgtgattt
    98881 cgtacacatc tgatttcact gtgagctgga atgaacttat aatgcgcttc caatactcta
    98941 atattctcaa cccaatggcc tgccaggcac aaaatctcgc ttaacatgaa tatgaaattg
    99001 aaaacattat tcgcagcggc cttcgctgtt gtcggctttt gcagtaccgc ctctgcggta
    99061 acttatcctc tgccaaccga cgggagtcgc ctggttggtc agaatcaggt gatcaccatt
    99121 cctgaaggta acactcagcc gctggagtat tttgccgcgg agtaccagat ggggctttcc
    99181 aatatgatgg aagcgaaccc gggtgtggat accttcctgc cgaaaggcgg tactgtactg
    99241 aacattccgc agcagctgat cctgccggat accgttcatg aaggcatcgt cattaacagt
    99301 gcagagatgc gtctgtatta ctatccgaaa gggaccaaca ccgttatcgt gctgccgatc
    99361 ggcattggtc agttaggcaa agatacgcct atcaactgga ccaccaaagt tgagcgtaag
    99421 aaagcaggcc cgacctggac gccgaccgcc aaaatgcacg cagagtaccg cgctgcgggc
    99481 gaaccgcttc cggctgtcgt tccggcaggt ccggataacc cgatgggtct gtatgcactc
    99541 tacatcggtc gcctgtatgc tatccatggc accaacgcca acttcggtat cggcctgcgt
    99601 gtaagtcatg gttgtgtgcg tctgcgtaac gaagacatca aattcctgtt cgagaaagta
    99661 ccggtcggta cccgcgtaca gtttattgat gagccggtaa aagcgaccac cgagccagac
    99721 ggcagccgtt atattgaagt ccataacccg ctgtctacca ccgaagccca gtttgaaggt
    99781 caggaaattg tgccaattac cctgacgaag aacgtgcaaa cagtgaccgg tcagccagat
    99841 gttgaccagg ttgttcttga tgaagcgatt aaaaaccgct ccgggatgcc ggttcgtctg
    99901 aattaatctt caaaacttaa agcaaaaggc ggactcataa tccgcctttt ttatttgcca
    99961 gaccctagtt ggccgggagt ataactaaca aaacatatcc caccaacgcc gcccacaaca
   100021 gcatcggtat tgaatagaga tggaaacgcc accagatgcg gcgatcgttc gccatacgta
   100081 gcgcaatcaa atttgccagc gatccgggta ataacccaaa gccacctacg tttaccgccc
   100141 ataccagtaa taaagacggc ggcacatagt tcagcaacaa tatggtactc ggcacgttac
   100201 tgatcacctg cgataaaccg attgccgtta accataaccc gggttcagat agatgactca
   100261 cgttacccaa cacgccttgc aacgctggaa gctgggtcag taaatggacg tcgataaaca
   100321 tcgccataaa caccagcagc agcgtccagt ccacactgag caccacgcga cgtgccagca
   100381 gcgcaaagcc tgccgccaca atcaccagtc cccacagctc ttgtttgaac tccagcgccg
   100441 tcagaaagac gatatacagc cccagacaac tccacaccag ccgcggtttc cactccggtg
   100501 tttgcacccc cgtatggtat tgcagcgcct ttccagggaa acaacaccag cacaggagca
   100561 ggagcgtcag catcattgcg ccagccagcg gtgccatttg ggcaataaat ccggcaaacg
   100621 aaagaccaga acgtccccag ataagaatat tttgcgggtt gccaattggc gtcagtagcg
   100681 aaccagcgtt gactgccagc gcctcaaaaa taatcagccg attaaccggg atctcacaca
   100741 gtctttttag cgtgatagtc agcggaacaa caataaacag cgcgacatcg ttggtcagaa
   100801 aggtagaaag cagcgccgcc gccagcacca taaacatcgc cagccgacgc tccgtagcaa
   100861 agcggcgcac cattttgcgc cccagcacat caaaataacc gcttaactcc acacctttgg
   100921 tcagcagcat caggccgctt aaggtgatga tggtgtgcca gtcgatagca gcaggccagg
   100981 atttcggtgc aaagggcacg aaaaagctta atccgatacc aacaagaatt aataactgaa
   101041 aaaaacgatc gccttgcagc gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg
   101101 ggtaaacaaa cgaaaagcgt cgagcgtctc ttcactaaca tggtgctcca tgccttccgc
   101161 gtcgcgacgg gcgatttccg gactgacgcc caacaccaac aagaaatttt cgactatctg
   101221 atgtcgctcg cggctttcct gcgccagctt ctctccttct gccgttaaaa acacgccacg
   101281 ccaggggatc atttcaatca gccccatggt tgccagccgc ttaagcattt tagccaccgt
   101341 cggttgcgaa actcccagac gagcagccat gtccacctga cgagcttccc ccacttccct
   101401 gatcaagtca gaaatcagct caacgtaatc atcaataagc tcgcgccgat gcgcctctct
   101461 gacctggcgg aacccttcaa cgtgctcttc cacgttcact aactgcgtca ctttttttgc
   101521 tgttggcgta cctgcgcgac gactcattgt gcttcctcat tatggtgacg catacatagc
   101581 gttctcattt cgtgcgcaca ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt
   101641 tagcaatagc tatataatat agcctgtgct atatctgtat gtaatgcaat catccctcaa
   101701 ggatcgacgg gattagcaag tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg
   101761 tgtttagtca ttctcccttt aaagtacggt taatgctgct ctctatgttg tgcgatatgg
   101821 tcaacaacaa accgcagcaa gataaacctt ccgataaata gcggcgtcgc ggtacgccgc
   101881 ttcactcctg ctttcatgca ggcataacgc gttttggtct gaaaaacccc actttttgtc
   101941 ggatttgcaa tccccttcgc aaaagatttg ttcgtcagta gttgacctga acggcggctc
   102001 gctctatctt cttgcagccc tgcgtatatt gcggctcgcg gatgcggacc cctttcccct
   102061 cttcacgcac tcttgcaggt attgaccctt gacgccaggg taagcacatg gcgtttgtta
   102121 cgatagtggc atatgaattt aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt
   102181 agcccgtgga ctgcgttcta ctttttacag tcgctattaa ttaacctcgg cttaggttac
   102241 cccttcagtt tgctctacac cgctgcgttt acggctattt tgcttttgct atggcgaaca
   102301 ttgcctcgcg tacaaaaagt tctggtcggt atcagttcgc tggtggcggc ttgttatttc
   102361 ccttttgctc aggcctacgg cgcgcctaac ttcaatacat tgctggcatt gcactccacc
   102421 aatatggaag agtcgaccga aatcctgacg atttttccgt ggtacagcta catggtcggc
   102481 atatttattt ttgtgctcgg cgtaatagca atcaggcgaa aaaaagagag tgaaaaagcg
   102541 cgctggaata ccttcgacag cctgtgtctg gtattcagtg tggcgacatt ttttgttgct
   102601 cccgtgcaaa acctggcctg gggtggcgta tttaaactga aagatactgg ctatccggta
   102661 tttcgttttg ctaaggatgt catcgtcaat aataacgagg tgattgaaga gcaagaacgg
   102721 atggcaaaac tttccggaat gaaagatacc tggacggtca ctgccgttaa gccgaagtat
   102781 cagacctatg tggtggtgat cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc
   102841 ggtcactggg acaatacccc gtttgccagc agcgttaacg gtttgatatt tgctgactac
   102901 attgccgcca gtggctccac gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat
   102961 ggcaaaccac agtttcagga taactttgtc accctggcaa atcgcgcggg cttccagacc
   103021 tggtggtttt ccaaccaggg ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc
   103081 aaacgtgcag atgaagtgta cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg
   103141 aaagacgaag cgttactgga tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg
   103201 cagctgattg ttctgcatct gatgggctcg catccgcagg cctgcgacag gacacaaggc
   103261 aaatacgaaa cctttgtgca atcgaaagaa acgtcgtgct atctctatac catgacgcaa
   103321 acggacgatt tactgcgcaa gctgtacgat cagttacgca acagcggcag cagcttctct
   103381 ctggtttact tttctgacca cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac
   103441 cttgcccatg atgataaata tcagcaaaat ttccaggtgc cttttatggt catttccagc
   103501 gacgataaag cgcatcgggt gattaaagcc cgccgctcag ccaatgactt cttaggcttt
   103561 ttctcgcagt ggacgggaat taaagcgaag gaaataaata tcaaataccc gtttatatct
   103621 gagaagaaag ccgggccgat atacatcacc aacttccagt tacagaaggt ggattacaac
   103681 catctcggaa ccgatatttt cgacccgaaa ccttaaaaca aaaaatccgc cccgagaggc
   103741 ggatttttta tatcaccaaa gtgattagaa gcggtaacca acaccggcaa tccaggtacc
   103801 tacgtcaacg ctacgaatac ggctctgctc gtaagagaag tccagagcaa cgttttccat
   103861 cgggttgaac tgcagaccag caccgtagga gaaaccgtag tcgctggtgt cgtgtttgta
   103921 ggtcgggtat tccgtggtct ggaatttacc ataacccacc cccactacac cgtagatgct
   103981 tgcccagtcg ttaatgcggt aagccggacc agcagtgatg ccgtagtact ggtttttgtt
   104041 gtagtcacca gagcttgcag tacggctttt ctcggtgtaa gtgaaagaac cgatcacacc
   104101 cagcgggctg ttgtcttctt catagcggta tttcaggttg aaaccgccca ttttgttcat
   104161 ttggccctga gcgtcgctct gtgcgtaacc gccagttacg gtagaagtcg cagctacgga
   104221 agtacctgcg gtgaaagcca gaactgcggc cagtgctgaa agacatgcaa tttttttcat
   104281 aaccacctca aatgtgattc aaataagtcc taagttttaa atatatcaaa aattaatggg
   104341 aaactcttcg cgatttgtga tgtctaacgg gccatttcat gtaacagaac gtttccatac
   104401 accgctatcc atctaaattt aaatcacttt ttcagagaac tgcgtaagta ttacgcatgt
   104461 tttccctgtc attcatccag attattccta atcaccagac taatgattcc atcaatcctg
   104521 gcgcatttta gtcaaaacgg gggaaaattt tttcaacaaa tgctcaacca tcattgggta
   104581 tatccagtac actccacgct ttacttaagt ctagatattt gtgggagaaa ggatgcctgg
   104641 ttcattacgt aaaatgccgg tctggttacc aataatcata ttgctcgttg ccatggcgtc
   104701 tattcagggt ggagcctcgt tagctaagtc actttttcct ctggtgggcg caccgggtgt
   104761 cactgcgctg cgtctggcat taggaacgct gatcctcatc gcgttcttta agccatggcg
   104821 actgcgcttt gccaaagagc aacggttacc gctgttgttt tacggcgttt cgctgggtgg
   104881 gatgaattat cttttttatc tttctattca gacagtaccg ctgggtattg cggtggcgct
   104941 ggagttcacc ggaccactgg cggtggcgct gttctcttct cgtcgcccgg tagatttcgt
   105001 ctgggttgtg ctggcggttc ttggtctgtg gttcctgcta ccgctggggc aagacgtttc
   105061 ccatgtcgat ttaaccggct gtgcgctggc actgggggcc ggggcttgtt gggctattta
   105121 cattttaagt gggcaacgcg caggagcgga acatggccct gcgacggtgg caattggttc
   105181 gttgattgca gcgttaattt tcgtgccaat tggagcgctt caggctggtg aagcactctg
   105241 gcactggtcg gttattccat tgggtctggc tgtcgctatt ctctcgaccg ctctgcctta
   105301 ttcgctggaa atgattgccc tcacccgttt gccaacacgg acatttggta cgctgatgag
   105361 catggaaccg gcgctggctg ccgtttccgg gatgattttc ctcggagaaa cactgacacc
   105421 catacagctg ctggcgctcg gcgctatcat cgccgcttca atggggtcta cgctgacagt
   105481 acgcaaagag agcaaaataa aagaattaga cattaattaa atttacattt ctgcatggtt
   105541 atgcataacc atgcagaatt tctcgctact tttcctctac accgtcttta tatatcgaat
   105601 tatgcaaaag catatttatt ccgaaaattc ctggcgagca gataaataag aattgttctt
   105661 atcaatatat ctaactcatt gaatctttat tagttttgtt tttcacgctt gttaccacta
   105721 ttagtgtgat aggaacagcc agaatagcgg aacacatagc cggtgctata cttagtctcg
   105781 ttaattactg ggacataaca tcaagaggat atgaaattat gagtaccgct aaattagtta
   105841 aatcaaaagc gaccaatctg ctttataccc gcaacgatgt ctccgacagc gagaaaaaag
   105901 caacagtaga gttgctgaat cgccaggtta tccagtttat tgatctttct ttgattacca
   105961 aacaagcgca ctggaacatg cgcggcgcta acttcattgc cgtacatgaa atgctggatg
   106021 gcttccgcac cgcactgatc gatcatctgg ataccatggc agaacgtgca gtgcagctgg
   106081 gcggtgtagc tctggggacc actcaagtta tcaacagcaa aaccccgctg aaaagttacc
   106141 cgctggacat ccacaacgtt caggatcacc tgaaagaact ggctgaccgt tacgcaatcg
   106201 tcgctaatga cgtacgcaaa gcgattggcg aagcgaaaga tgacgacacc gcagatatcc
   106261 tgaccgccgc gtctcgcgac ctggataaat tcctgtggtt tatcgagtct aacatcgaat
   106321 aaatccatcg ctgatggtgc agaactttag tacccgataa aagcggcttc ctgacaggag
   106381 gccgttttgt tttgcagccc acctcaacgc acttatttag tgcatccatc tgctatctcc
   106441 agctgattaa gtaaattttt tgtatccaca tcatcacaca atcgttacat aaagattgtt
   106501 ttttcatcag gttttacgct aaataatcac tgtgttgagt gcacaatttt agcgcaccag
   106561 attggtgccc cagaatggtg catcttcagg gtattgccct ataaatcgtg catcacgttt
   106621 ttgccgcatc tcgaaaaatc aatgagttgc aaaactggca cgattttttc atatatgtga
   106681 atgtcacgca ggggatcgtc ccgtggatag aaaaaaggaa atgctatgaa gtctgtatta
   106741 aaagtttcac tggctgcact gaccctggct tttgcggttt cttctcatgc cgcggataaa
   106801 aaattagttg tcgcgacgga taccgccttc gttccgtttg aatttaaaca gggcgataaa
   106861 tatgtgggct ttgacgttga tctgtgggct gccatcgcta aagagctgaa gctggattac
   106921 gaactgaagc cgatggattt cagtgggatc attccggcac tgcaaaccaa aaacgtcgat
   106981 ctggcgctgg cgggcattac catcaccgac gagcgtaaaa aagcgatcga tttctctgac
   107041 ggctactaca aaagcggcct gttagtgatg gtgaaagcta acaataacga tgtgaaaagc
   107101 gtgaaagatc tcgacgggaa agtggttgct gtgaagagcg gtactggctc cgttgattac
   107161 gcgaaagcaa acatcaaaac taaagatctg cgtcagttcc cgaacatcga taacgcctat
   107221 atggaactgg gcaccaaccg cgcagacgcc gttctgcacg atacgccaaa cattctgtac
   107281 ttcatcaaaa ccgccggtaa cggtcagttc aaagcggtag gtgactctct ggaagcgcag
   107341 caatacggta ttgcgttccc gaaaggtagc gacgagctgc gtgacaaagt caacggcgcg
   107401 ttgaaaaccc tgcgcgagaa cggaacttac aacgaaatct acaaaaaatg gttcggtact
   107461 gaaccgaaat aataacgcta cacctgtaaa acgcactggc agttccctct cccctctggg
   107521 gagaggatta gggtgagggg cgcaaacccg ctccggggcc attaattacc ctgaatttga
   107581 ttatttacac cacggtaaca ggaacaacat atgcagtttg actggagtgc catctggcct
   107641 gccattccgc ttctgattga aggtgccaaa atgaccctgt ggatttcggt cctcggtctg
   107701 gcaggcggtc tggtaatcgg attgctggca ggttttgcac gcaccttcgg tggttggata
   107761 gccaaccacg tcgcgctggt ctttattgaa gtgatccgcg gcacacctat cgtcgtccag
   107821 gtgatgttta tctatttcgc cctgccgatg gcgtttaacg acttacgcat cgacccattt
   107881 actgcggcgg tggtcaccat catgatcaac tccggcgcgt acattgcgga aatcacgcgt
   107941 ggtgcggtgc tgtctatcca caaaggtttt cgtgaagcag gactggcgct cggtctttca
   108001 cgttgggaaa ccattcgcta cgtcatttta ccgctggcac tgcgtcgtat gctgccgccg
   108061 ctgggtaacc agtggatcat cagcattaaa gacacctcgc tgtttattgt gatcggcgtg
   108121 gcggaactga cccgtcaggg gcaagaaatt attgccggta acttccgcgc ccttgagatc
   108181 tggagcgccg tggcggtgct ctatctgatt attaccctgg tgctgagctt tattctgcgt
   108241 cgtctggaaa gaaggatgaa aatcctgtga ttgaatttaa aaacgtctcc aagcactttg
   108301 gcccaaccca ggtgctgcat aatatcgatt tgaacattgc ccagggcgaa gtcgtggtga
   108361 ttatcgggcc gtccggttcc ggtaaatcga ccctgctgcg ctgcatcaac aaactggaag
   108421 aaatcacctc cggcgatctg attgtcgatg gcctgaaggt taacgatccg aaagttgacg
   108481 agcgcctgat tcgccaggaa gcaggtatgg tgttccagca gttttacctc ttcccgcatc
   108541 tgacggcgct ggaaaacgtc atgtttggtc cgctacgcgt gcgtggcgcg aacaaagaag
   108601 aggcggaaaa actggcgcgt gagctgctgg cgaaagtcgg tctggcagaa cgtgcacatc
   108661 actacccttc cgaactttct ggtggtcaac agcagcgtgt ggcgattgcc cgcgcgctgg
   108721 cggtgaagcc gaaaatgatg ctgtttgatg aaccgacttc cgctcttgac ccggaactgc
   108781 gccatgaagt gctgaaggtt atgcaggatc tggctgaaga agggatgacg atggtgatcg
   108841 tgacccacga aatcggtttt gccgagaaag tagcttcgcg gctgatcttt atcgacaaag
   108901 gccggattgc ggaagatggc gatccgcagg tattgatcaa gaacccgccg agccagcgct
   108961 tgcaggaatt tttgcagcac gtctcttaac ttctctgcct ccctttcacc cgaaagggag
   109021 gcacaccaga ttcctctcat ttaaaatcgc ccctccccca gcatctatac ttatcttttt
   109081 gctctgtttt ctcactggag gagtcatgcg gtggatcctg ttcatcctct tctgcctgct
   109141 gggcgcacct gcccacgcgg tatccatacc cggcgttaca accacaacga caacggactc
   109201 aacgactgaa ccggccccgg aaccggatat cgaacaaaaa aaagcggcct atggcgcact
   109261 ggcggatgtg ctggataacg acacctcgcg taaagaattg atcgaccagt tgcgtactgt
   109321 cgccgcgacg ccccctgcgg aaccggtccc gaagatcgtg ccgccgacgc tggttgaaga
   109381 gcaaaccgtg ctgcaaaagg tcaccgaagt cagccgccat tatggtgaag ccctttccgc
   109441 ccgcttcggg caactttatc gcaatatcac cggctccccg cataagccat ttaatccaca
   109501 aaccttcagc aatgcgctga gccatttttc aatgttagcg gtattagtgt ttggttttta
   109561 ctggctgatt cgcctgtgcg cactgccgct gtatcgcaaa atgggtcagt gggcgaggca
   109621 aaaaaatcgt gagcgcagca actggttgca gcttccggcg atgattatcg gggcgtttat
   109681 tatcgacctg ctgttactgg ccctgacatt atttgtcggc caggtattaa gcgacaacct
   109741 gaatgcaggc agtcgcacca tcgctttcca acaaagtttg tttctcaatg cctttgccct
   109801 gattgaattt ttcaaagccg tactacgcct gattttttgc ccaaacgtgg cggagctgcg
   109861 tccgttcacg attcaggacg agagcgcccg ttactggagt cgtcgcctga gctggttaag
   109921 cagtttgata ggttatggcc tgattgtggc cgtgccgatt atctctaatc aggtgaatgt
   109981 acagataggt gcgctggcga acgtcatcat tatgctgtgc atgaccgtct gggcgttgta
   110041 cctgatcttt cgtaataaaa aagagattac ccagcatttg ctcaactttg cggagcattc
   110101 gctggccttt ttcagcctgt ttatccgcgc ctttgcgctg gtgtggcact ggctggcaag
   110161 cgcctatttt atcgtgctgt ttttcttttc gttgttcgat ccgggcaaca gcctgaaatt
   110221 tatgatgggt gcaacggtgc gcagcctggc gattattggt atcgcagcgt ttgtttccgg
   110281 tatgttttcc cgctggctgg cgaaaaccat caccctctcg ccacatactc agcgtaacta
   110341 tccggagcta caaaaacggc tgaatggctg gctttcggcg gcgctgaaaa cggcgcgtat
   110401 tctgaccgtc tgcgtggcag taatgttgct gttgagcgca tggggattgt tcgatttctg
   110461 gaactggctg caaaacggcg cggggcagaa aaccgtagat atcctgatcc gcatcgcact
   110521 cattcttttc ttctcggcgg ttggctggac ggtgctcgcc agtttgatcg aaaaccggct
   110581 ggcttcggat attcatggcc gcccgctacc cagcgcccgt acgcgtaccc tgctgacgct
   110641 gtttcgtaac gcgctggcgg tgattatcag taccatcacc atcatgattg tgttgtcgga
   110701 aatcggcgtc aatatcgcgc cattgctggc aggtgccgga gcattaggtc tggctatctc
   110761 gtttggttcg caaacgctgg tgaaagatat cattaccggg gtgtttattc agtttgaaaa
   110821 cggcatgaac actggagatt tggtgactat cgggccgttg accggcacag tggaacggat
   110881 gtcgattcgc tccgtgggcg tgcgccagga taccggggcg tatcacatca ttccgtggtc
   110941 ttcgataacc acctttgcta acttcgtccg cggtattggt tcggtagtgg caaactacga
   111001 tgttgatcgc catgaagatg ctgataaagc caatcaggca ctgaaagatg cggtagcgga
   111061 attgatggaa aacgaagaaa ttcgcgggct gattattggt gaaccgaatt ttgccgggat
   111121 tgtcggctta agcaataccg cgtttacact gcgtgtttcg ttcaccacgc tgccactcaa
   111181 acagtggacg gtacgctttg ccctcgacag ccaggtgaaa aaacatttcg acctggcggg
   111241 cgttcgcgcg ccagtgcaga cttatcaggt gctgcctgct ccgggcgcga ccccggctga
   111301 accgccgccg ccgggggagc caacgcttta acgttggcga ttaacaaaac ggcggcgctg
   111361 ctcgtcgttc ataaaggtcc aggcaataaa gcgactctgc ttttgcccct gggccatctc
   111421 tttttttacc accttcaccg cgcccacgtc ggtcagagca cgatacaacg gcggtaagtt
   111481 ttcaccacga gataccagcg aggtaaacca catcacctgc ttcgcgaagc ctttgctctc
   111541 ttcaatcatc tttttgataa aggcgacttc accaccttca caccacaact cctgttgctg
   111601 accaccaaag ttcagttcat cgtctttgtt cagccccagg ttacggcgtt tacgctcact
   111661 acctgcccgc gctgcagcgg cggaatcgtg gaatggcggg ttacacaagg tcgcgtcgta
   111721 ttgctcgttt ttatggatga tgccgttaaa aattgcccca ctctcttttt gccgacgcag
   111781 acgaatggcg cggttaagcc ccggattagc actgataatc gcctgcgcac tgcttaacgc
   111841 ctggctgctg gtttcactac cggtaaaacg ccagccatat tcatgtacgc caatcagcgg
   111901 ataaatacag ttcgcaccaa cgccgatatc cagaatgctg gcattcgctg gaattgttcc
   111961 gcttgcttca gccagtaaat cggcaaggtg atgaatataa tccgcccggc ccggtactgg
   112021 tgggcagaga aaaccgtcgg ggatatccca gttcgctacg gcgtaaaaat gggccagcaa
   112081 cgccttattg agcgccttca ccgccagcgg attggcaaag tctacgcttt gctccccggc
   112141 gggtgtaagc gtgaggaatt gcctgagttc aggattgacc tgacaaagcg tggcgagatc
   112201 gtagcggctg tgatgacggt tgcgcggatg caaccccggt ttctgggcgg acatagcatt
   112261 ctcctttaag cagcggcgta agatacccgc ataacgcgac agggtaaata tctggctgcg
   112321 tttatcattc cccaaaacct taacatggct atggtttatg gcaattcatc ataaaaatca
   112381 gcattatgca tgtcattgat ttgtcttatt ttgtaaaaat atatcgttca aaaaataagc
   112441 acatctctgt caattttttg cgtcattttc atcgcccagg cgacctacac ttatcaggca
   112501 ctacccgttg tatttatgag gatgtcatta tgaaaaagtg cctcacacta ctgattgcca
   112561 ccgtcctgag cggaatctct ctcacggctt atgccgcgca accgatgagt aacctggaca
   112621 gcggtcagct acggcccgcc ggtaccgttt cggcaactgg cgcatcaaac ctaagcgatc
   112681 tggaggataa actggcagaa aaagcgcgcg agcaaggcgc gaaaggttat gtcattaatt
   112741 ccgcaggcgg aaatgaccag atgttcggta ctgcaaccat ctacaaataa ccccaccaac
   112801 gtaactgcat tcgcctctgg atgcagtttt cgcctgtttt gtaatcaaaa aacatcaaat
   112861 atgaacgcaa tgtaaataaa tgtatttctt tttcgcgcaa tgggtgatag aaaatcgcgc
   112921 caagtgataa tgcttatcaa aattattatc acttgcacga acactatcac gagattaaca
   112981 gtggcatcgc atccgcagag aggctttctc gtggcagtga aaatttcaac atataaaaaa
   113041 agtcacctgc aaaatggaaa acaatcgcaa tttccctgcc agacaatttc attcgctcac
   113101 gttctttgcc ggtctttgta ttggcatcac gcctgtggct caggcactcg ccgccgaagg
   113161 gcaaactaac gcggatgaca cgctggttgt cgaagcatca acgccttcgc tttatgcgcc
   113221 acaacaatct gccgatccga aattctcgcg tccggtagcg gatactaccc gcacgatgac
   113281 ggtgatttct gaacaagtga ttaaagatca gggcgcaacc aaccttaccg acgcgctcaa
   113341 aaacgtcccc ggcgtgggtg cgttttttgc gggtgagaac ggtaactcca ccactggcga
   113401 cgccatttat atgcgcggtg ctgatacctc taacagtatt tatattgatg gcattcgcga
   113461 tatcggcagc gtctcgcgcg acaccttcaa tactgagcag gtcgaagtga ttaaagggcc
   113521 ttccggcacc gactacgggc gcagcgcgcc gacgggctcg atcaatatga tcagcaagca
   113581 gccgcgcaat gattccggca ttgacgcctc cgccagtatt ggcagcgcct ggttccgccg
   113641 cggcacgctg gacgtcaatc aggtcattgg tgataccact gcggtgcgcc tgaatgtgat
   113701 gggcgaaaaa acgcacgatg ccggacgcga caaagtcaaa aatgagcgtt acggcgtcgc
   113761 cccttctatc gcttttggcc ttggtacagc gaatcgtttg tatcttaatt atctgcatgt
   113821 cacccagcac aacacgccag acggcggcat tccgaccatc ggtttgccgg gttattctgc
   113881 cccatctgcg ggaacagcgg ctctgaatca ttccggaaaa gttgatactc ataactttta
   113941 cggcacggat tccgattacg acgattcgac caccgacacc gccaccatgc gttttgagca
   114001 cgacatcaac gataacacca ccattcgcaa tactacccgt tggtcgcgcg taaagcagga
   114061 ttacctgatg acggcgatta tgggcggggc gtcgaatatt actcagccca ccagcgatgt
   114121 gaatagctgg acctggtcac gcacggcgaa taccaaagat gtgagtaata aaattctcac
   114181 caaccagacc aacctgacct cgacgttcta taccggttct atcggtcatg atgtcagtac
   114241 cggcgtggaa tttacccgtg aaacgcagac taactacggc gttaatccgg tgacgttacc
   114301 cgcggtaaat atttatcatc ctgacagcag cattcatccc ggcggcctga cgcgcaacgg
   114361 cgtaaacgcc aatggtcaga cggatacctt cgcaatttac gccttcgata cgctgcaaat
   114421 cacccgtgat tttgagctga acggcgggat ccgtctggat aattatcata ctgaatatga
   114481 cagtgccacc gcctgcggcg gcagcggacg cggtgccatc acctgcccaa ctggtgtggc
   114541 aaaaggttct ccggtcacca ccgtcgacac cgccaagtcg ggcaatctgg tgaactggaa
   114601 agccggggcg ctgtatcacc tgacggaaaa cggtaatgtc tatattaact atgccgtttc
   114661 ccagcagcct ccgggcggca acaacttcgc ccttgcgcag tctggcagcg gtaacagtgt
   114721 caaccgcacc gattttaaac cgcaaaaagc caacaccagc gagattggca ccaaatggca
   114781 ggttctggat aaacgcctgt tgctcaccgc cgcgctattc cgtactgata tcgaaaatga
   114841 agttgagcaa aatgatgacg gaacttactc gcaatacggt aagaaacgtg tcgaaggcta
   114901 tgagatatcc gtggccggga atatcacgcc cgcgtggcag gtgattggcg gctataccca
   114961 gcaaaaagca accatcaaaa acggcaaaga tgttgcccag gatggttcct catcgctgcc
   115021 gtataccccg gagcacgcct tcaccttatg gagccagtat caggcaacag atgatatctc
   115081 tgttggcgcg ggcgcacgct atatcggcag tatgcataaa ggttcagacg gcgcggtggg
   115141 aacgccagcg tttaccgaag gttactgggt cgccgatgcc aaactggggt atcgggttaa
   115201 tcgcaatctc gacttccagc taaacgttta caacctgttt gataccgatt acgtcgcctc
   115261 aatcaacaag agcggctacc gttatcaccc gggcgagcca agaaccttct tgctcacagc
   115321 caatatgcat ttctgattca gatgtggggc gcaggcccca ctttttggag aacttgtatg
   115381 atgtaccaca ttcccggcgt gttatcgcca caggacgtcg cccggtttcg cgaacaactg
   115441 gaacaagccg aatgggtgga tggacgcgtc accaccggcg cacaaggtgc gcaagttaag
   115501 aacaatcaac aggtcgacac ccgcagcacg ttatacgccg ccctgcaaaa tgaggtgctg
   115561 aacgcggtta accaacatgc tttattcttt gccgcggcct tgccgcgtac cctttccacg
   115621 ccgctgttta atcgctatca gaacaatgaa acctatggtt tccatgtgga tggcgcagta
   115681 cgcagccatc cacaaaacgg ctggatgcgt actgaccttt ctgccacgct gtttttaagc
   115741 gatccacaaa gctacgacgg cggcgaactg gtcgttaatg acaccttcgg acaacatcgg
   115801 gtaaaactcc cggcaggcga tctcgtgttg tatccctcca gcagcctgca ttgcgtgaca
   115861 cccgtaaccc gcggcgtacg agtggcatca tttatgtgga tccagtcgat gatccgtgat
   115921 gataaaaagc gcgccatgct gtttgaactg gacaacaata ttcagtcgct gaaaagccgc
   115981 tacggcgaaa gtgaagagat cctgtcgctg cttaatcttt atcataatct gctgcgggaa
   116041 tggtcggaga tctgatgctt gaactgccca ggttcgtccc tggattagct acacttaact
   116101 gtacaagcat tgatatgggg aggacgatat ggcatccggt tgggctaacg atgacgccgt
   116161 caacgaacag atcaacagta caattgaaga tgcgattgcc cgcgctcggg gtgaaattcc
   116221 gcgtggcgaa agcctggatg aatgtgaaga gtgcggtgcc cccatcccgc aagcccgtcg
   116281 ggaagccatt cctggcgtgc gcttatgcat tcattgtcag caggagaaag atttacaaaa
   116341 accagcgtat acaggatata atcgcagagg ttcgaaagac agccagttac gttaactaca
   116401 actggcgaga tgcatagcga gtataatttg tattttgcgt ggcattccgt gcctttaacg
   116461 ctgctgtg
//
LOCUS       DAZGTR010000002       109155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGTR010000002 DAZGTR010000000
VERSION     DAZGTR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.002"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(93..353)
                     /gene="yfhL"
                     /locus_tag="ACHLCY_000105"
     CDS             complement(93..353)
                     /gene="yfhL"
                     /locus_tag="ACHLCY_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP0367440.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(409..1257)
                     /gene="yfhH"
                     /locus_tag="ACHLCY_000106"
     CDS             complement(409..1257)
                     /gene="yfhH"
                     /locus_tag="ACHLCY_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0367441.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            1466..2101
                     /gene="yfhb"
                     /locus_tag="ACHLCY_000107"
     CDS             1466..2101
                     /gene="yfhb"
                     /locus_tag="ACHLCY_000107"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP0367442.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            2159..2662
                     /gene="tadA"
                     /locus_tag="ACHLCY_000108"
     CDS             2159..2662
                     /gene="tadA"
                     /locus_tag="ACHLCY_000108"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP0367443.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(2659..4215)
                     /gene="mltF"
                     /locus_tag="ACHLCY_000109"
     CDS             complement(2659..4215)
                     /gene="mltF"
                     /locus_tag="ACHLCY_000109"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP0367444.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            4473..8360
                     /gene="purL"
                     /locus_tag="ACHLCY_000110"
                     /gene_synonym="purI"
     CDS             4473..8360
                     /gene="purL"
                     /locus_tag="ACHLCY_000110"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP0367445.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELYLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            8936..10363
                     /gene="qseE"
                     /locus_tag="ACHLCY_000111"
     CDS             8936..10363
                     /gene="qseE"
                     /locus_tag="ACHLCY_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP0367446.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            10528..11241
                     /gene="qseG"
                     /locus_tag="ACHLCY_000112"
     CDS             10528..11241
                     /gene="qseG"
                     /locus_tag="ACHLCY_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP0367447.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            11231..12565
                     /gene="glrR"
                     /locus_tag="ACHLCY_000113"
     CDS             11231..12565
                     /gene="glrR"
                     /locus_tag="ACHLCY_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP0367448.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            12626..12964
                     /gene="glnB"
                     /locus_tag="ACHLCY_000114"
     CDS             12626..12964
                     /gene="glnB"
                     /locus_tag="ACHLCY_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP0367449.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(13009..14199)
                     /gene="hmpA"
                     /locus_tag="ACHLCY_000115"
     CDS             complement(13009..14199)
                     /gene="hmpA"
                     /locus_tag="ACHLCY_000115"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP0367450.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            14527..15780
                     /gene="glyA"
                     /locus_tag="ACHLCY_000116"
     CDS             14527..15780
                     /gene="glyA"
                     /locus_tag="ACHLCY_000116"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP0367451.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            complement(15844..17037)
                     /gene="yphH"
                     /locus_tag="ACHLCY_000117"
     CDS             complement(15844..17037)
                     /gene="yphH"
                     /locus_tag="ACHLCY_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP0367452.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVSTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            17155..20436
                     /gene="yphG"
                     /locus_tag="ACHLCY_000118"
                     /pseudo
     CDS             17155..20436
                     /gene="yphG"
                     /locus_tag="ACHLCY_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            20533..21516
                     /gene="yphF"
                     /locus_tag="ACHLCY_000119"
                     /pseudo
     CDS             20533..21516
                     /gene="yphF"
                     /locus_tag="ACHLCY_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            21539..23050
                     /gene="yphE"
                     /locus_tag="ACHLCY_000120"
     CDS             21539..23050
                     /gene="yphE"
                     /locus_tag="ACHLCY_000120"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0367453.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVIIAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQVIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHAPVNVDELMSAILSVH"
     gene            23075..24073
                     /gene="yphD"
                     /locus_tag="ACHLCY_000121"
     CDS             23075..24073
                     /gene="yphD"
                     /locus_tag="ACHLCY_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0367454.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            24106..25200
                     /gene="yphC"
                     /locus_tag="ACHLCY_000122"
     CDS             24106..25200
                     /gene="yphC"
                     /locus_tag="ACHLCY_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0367455.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            25212..26084
                     /gene="yphB"
                     /locus_tag="ACHLCY_000123"
     CDS             25212..26084
                     /gene="yphB"
                     /locus_tag="ACHLCY_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0367456.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(26132..26554)
                     /gene="yphA"
                     /locus_tag="ACHLCY_000124"
     CDS             complement(26132..26554)
                     /gene="yphA"
                     /locus_tag="ACHLCY_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0367457.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(26651..27853)
                     /gene="hcaD"
                     /locus_tag="ACHLCY_000125"
     CDS             complement(26651..27853)
                     /gene="hcaD"
                     /locus_tag="ACHLCY_000125"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP0367458.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            complement(27863..28675)
                     /gene="hcaB"
                     /locus_tag="ACHLCY_000126"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(27863..28675)
                     /gene="hcaB"
                     /locus_tag="ACHLCY_000126"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP0367459.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(28672..28992)
                     /gene="hcaC"
                     /locus_tag="ACHLCY_000127"
     CDS             complement(28672..28992)
                     /gene="hcaC"
                     /locus_tag="ACHLCY_000127"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP0367460.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(28992..29510)
                     /gene="hcaF"
                     /locus_tag="ACHLCY_000128"
     CDS             complement(28992..29510)
                     /gene="hcaF"
                     /locus_tag="ACHLCY_000128"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP0367461.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(29507..30868)
                     /gene="hcaE"
                     /locus_tag="ACHLCY_000129"
     CDS             complement(29507..30868)
                     /gene="hcaE"
                     /locus_tag="ACHLCY_000129"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP0367462.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            31004..31894
                     /gene="hcaR"
                     /locus_tag="ACHLCY_000130"
     CDS             31004..31894
                     /gene="hcaR"
                     /locus_tag="ACHLCY_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP0367463.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            32054..33193
                     /gene="hcaT"
                     /locus_tag="ACHLCY_000131"
     CDS             32054..33193
                     /gene="hcaT"
                     /locus_tag="ACHLCY_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP0367464.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(33185..34465)
                     /gene="csiE"
                     /locus_tag="ACHLCY_000132"
     CDS             complement(33185..34465)
                     /gene="csiE"
                     /locus_tag="ACHLCY_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP0367465.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAKSCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(34656..35510)
                     /gene="yfhR"
                     /locus_tag="ACHLCY_000133"
     CDS             complement(34656..35510)
                     /gene="yfhR"
                     /locus_tag="ACHLCY_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0367466.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(35655..36458)
                     /gene="suhB"
                     /locus_tag="ACHLCY_000134"
     CDS             complement(35655..36458)
                     /gene="suhB"
                     /locus_tag="ACHLCY_000134"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP0367467.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            36577..37314
                     /gene="trmJ"
                     /locus_tag="ACHLCY_000135"
     CDS             36577..37314
                     /gene="trmJ"
                     /locus_tag="ACHLCY_000135"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP0367468.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFSR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            37615..38103
                     /gene="iscR"
                     /locus_tag="ACHLCY_000136"
     CDS             37615..38103
                     /gene="iscR"
                     /locus_tag="ACHLCY_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP0367469.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            38344..39558
                     /gene="iscS"
                     /locus_tag="ACHLCY_000137"
     CDS             38344..39558
                     /gene="iscS"
                     /locus_tag="ACHLCY_000137"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP0367470.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            39586..39972
                     /gene="iscU"
                     /locus_tag="ACHLCY_000138"
     CDS             39586..39972
                     /gene="iscU"
                     /locus_tag="ACHLCY_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP0367471.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            39989..40312
                     /gene="iscA"
                     /locus_tag="ACHLCY_000139"
     CDS             39989..40312
                     /gene="iscA"
                     /locus_tag="ACHLCY_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP0367472.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            40408..40923
                     /gene="hscB"
                     /locus_tag="ACHLCY_000140"
     CDS             40408..40923
                     /gene="hscB"
                     /locus_tag="ACHLCY_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP0367473.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            40940..42790
                     /gene="hscA"
                     /locus_tag="ACHLCY_000141"
     CDS             40940..42790
                     /gene="hscA"
                     /locus_tag="ACHLCY_000141"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP0367474.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            42792..43127
                     /gene="fdx"
                     /locus_tag="ACHLCY_000142"
     CDS             42792..43127
                     /gene="fdx"
                     /locus_tag="ACHLCY_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP0367475.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            43139..43339
                     /gene="iscX"
                     /locus_tag="ACHLCY_000143"
     CDS             43139..43339
                     /gene="iscX"
                     /locus_tag="ACHLCY_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP0367476.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            43517..44800
                     /gene="pepB"
                     /locus_tag="ACHLCY_000144"
     CDS             43517..44800
                     /gene="pepB"
                     /locus_tag="ACHLCY_000144"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP0367477.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            44942..45718
                     /gene="sseB"
                     /locus_tag="ACHLCY_000145"
     CDS             44942..45718
                     /gene="sseB"
                     /locus_tag="ACHLCY_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP0367478.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(45750..45875)
                     /gene="timP"
                     /locus_tag="ACHLCY_000146"
     CDS             complement(45750..45875)
                     /gene="timP"
                     /locus_tag="ACHLCY_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP0367479.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(46211..47056)
                     /gene="sseA"
                     /locus_tag="ACHLCY_000147"
     CDS             complement(46211..47056)
                     /gene="sseA"
                     /locus_tag="ACHLCY_000147"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP0367480.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            47263..52224
                     /gene="yfhM"
                     /locus_tag="ACHLCY_000148"
     CDS             47263..52224
                     /gene="yfhM"
                     /locus_tag="ACHLCY_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP0367481.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMVLNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANDVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            52225..54537
                     /gene="pbpC"
                     /locus_tag="ACHLCY_000149"
     CDS             52225..54537
                     /gene="pbpC"
                     /locus_tag="ACHLCY_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP0367482.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGAWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            54686..55117
                     /gene="ndk"
                     /locus_tag="ACHLCY_000150"
     CDS             54686..55117
                     /gene="ndk"
                     /locus_tag="ACHLCY_000150"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP0367483.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            55267..56421
                     /gene="rlmN"
                     /locus_tag="ACHLCY_000151"
     CDS             55267..56421
                     /gene="rlmN"
                     /locus_tag="ACHLCY_000151"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP0367484.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRDKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            56706..57719
                     /gene="rodZ"
                     /locus_tag="ACHLCY_000152"
     CDS             56706..57719
                     /gene="rodZ"
                     /locus_tag="ACHLCY_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP0367485.1"
                     /translation="MNTEATHDQNEALTTGARLHNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            57746..58864
                     /gene="ispG"
                     /locus_tag="ACHLCY_000153"
                     /gene_synonym="gcpE"
     CDS             57746..58864
                     /gene="ispG"
                     /locus_tag="ACHLCY_000153"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP0367486.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            58975..60249
                     /gene="hisS"
                     /locus_tag="ACHLCY_000154"
     CDS             58975..60249
                     /gene="hisS"
                     /locus_tag="ACHLCY_000154"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP0367487.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            60267..60887
                     /gene="yfgM"
                     /locus_tag="ACHLCY_000155"
     CDS             60267..60887
                     /gene="yfgM"
                     /locus_tag="ACHLCY_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP0367488.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            60898..62076
                     /gene="bamB"
                     /locus_tag="ACHLCY_000156"
     CDS             60898..62076
                     /gene="bamB"
                     /locus_tag="ACHLCY_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP0367489.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            62194..63666
                     /gene="der"
                     /locus_tag="ACHLCY_000157"
     CDS             62194..63666
                     /gene="der"
                     /locus_tag="ACHLCY_000157"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP0367490.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            63735..63950
                     /gene="yfgJ"
                     /locus_tag="ACHLCY_000158"
     CDS             63735..63950
                     /gene="yfgJ"
                     /locus_tag="ACHLCY_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0367491.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(63947..65317)
                     /gene="xseA"
                     /locus_tag="ACHLCY_000159"
     CDS             complement(63947..65317)
                     /gene="xseA"
                     /locus_tag="ACHLCY_000159"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP0367492.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRLFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            65479..66945
                     /gene="guaB"
                     /locus_tag="ACHLCY_000160"
     CDS             65479..66945
                     /gene="guaB"
                     /locus_tag="ACHLCY_000160"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP0367493.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTTKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            67014..68591
                     /gene="guaA"
                     /locus_tag="ACHLCY_000161"
     CDS             67014..68591
                     /gene="guaA"
                     /locus_tag="ACHLCY_000161"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP0367494.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(68686..69225)
                     /gene="yfgI"
                     /locus_tag="ACHLCY_000162"
     CDS             complement(68686..69225)
                     /gene="yfgI"
                     /locus_tag="ACHLCY_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP0367495.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKRVGKAEENKSDSWFN"
     gene            complement(69241..69759)
                     /gene="yfgH"
                     /locus_tag="ACHLCY_000163"
     CDS             complement(69241..69759)
                     /gene="yfgH"
                     /locus_tag="ACHLCY_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0367496.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(70070..70261)
                     /gene="yfgG"
                     /locus_tag="ACHLCY_000164"
     CDS             complement(70070..70261)
                     /gene="yfgG"
                     /locus_tag="ACHLCY_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP0367497.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            70279..70431
                     /locus_tag="ACHLCY_000165"
     CDS             70279..70431
                     /locus_tag="ACHLCY_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367498.1"
                     /translation="MPHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            70613..72856
                     /gene="pdeF"
                     /locus_tag="ACHLCY_000166"
     CDS             70613..72856
                     /gene="pdeF"
                     /locus_tag="ACHLCY_000166"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP0367499.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(72895..74436)
                     /gene="ppx"
                     /locus_tag="ACHLCY_000167"
     CDS             complement(72895..74436)
                     /gene="ppx"
                     /locus_tag="ACHLCY_000167"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP0367500.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(74441..76507)
                     /gene="ppk1"
                     /locus_tag="ACHLCY_000168"
     CDS             complement(74441..76507)
                     /gene="ppk1"
                     /locus_tag="ACHLCY_000168"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP0367501.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANVQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(76678..77316)
                     /gene="purN"
                     /locus_tag="ACHLCY_000169"
     CDS             complement(76678..77316)
                     /gene="purN"
                     /locus_tag="ACHLCY_000169"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP0367502.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(77316..78353)
                     /gene="purM"
                     /locus_tag="ACHLCY_000170"
     CDS             complement(77316..78353)
                     /gene="purM"
                     /locus_tag="ACHLCY_000170"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP0367503.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            78678..79304
                     /gene="upp"
                     /locus_tag="ACHLCY_000171"
     CDS             78678..79304
                     /gene="upp"
                     /locus_tag="ACHLCY_000171"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP0367504.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            79390..80679
                     /gene="uraA"
                     /locus_tag="ACHLCY_000172"
     CDS             79390..80679
                     /gene="uraA"
                     /locus_tag="ACHLCY_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP0367505.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            80774..81475
                     /gene="hda"
                     /locus_tag="ACHLCY_000173"
     CDS             80774..81475
                     /gene="hda"
                     /locus_tag="ACHLCY_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP0367506.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(81613..81972)
                     /gene="arsC"
                     /locus_tag="ACHLCY_000174"
     CDS             complement(81613..81972)
                     /gene="arsC"
                     /locus_tag="ACHLCY_000174"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP0367507.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(81993..83456)
                     /gene="bepA"
                     /locus_tag="ACHLCY_000175"
     CDS             complement(81993..83456)
                     /gene="bepA"
                     /locus_tag="ACHLCY_000175"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP0367508.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            83669..84730
                     /gene="yfgO"
                     /locus_tag="ACHLCY_000176"
     CDS             83669..84730
                     /gene="yfgO"
                     /locus_tag="ACHLCY_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0367509.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(84768..85616)
                     /gene="focB"
                     /locus_tag="ACHLCY_000177"
     CDS             complement(84768..85616)
                     /gene="focB"
                     /locus_tag="ACHLCY_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP0367510.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRAI
                     YLRQES"
     gene            complement(85638..87650)
                     /gene="hyfR"
                     /locus_tag="ACHLCY_000178"
     CDS             complement(85638..87650)
                     /gene="hyfR"
                     /locus_tag="ACHLCY_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP0367511.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(87680..88093)
                     /gene="hyfJ"
                     /locus_tag="ACHLCY_000179"
     CDS             complement(87680..88093)
                     /gene="hyfJ"
                     /locus_tag="ACHLCY_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP0367512.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(88086..88843)
                     /gene="hyfI"
                     /locus_tag="ACHLCY_000180"
                     /pseudo
     CDS             complement(88086..88843)
                     /gene="hyfI"
                     /locus_tag="ACHLCY_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(88840..89385)
                     /gene="hyfH"
                     /locus_tag="ACHLCY_000181"
     CDS             complement(88840..89385)
                     /gene="hyfH"
                     /locus_tag="ACHLCY_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP0367513.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(89395..91062)
                     /gene="hyfG"
                     /locus_tag="ACHLCY_000182"
     CDS             complement(89395..91062)
                     /gene="hyfG"
                     /locus_tag="ACHLCY_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP0367514.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(91052..92632)
                     /gene="hyfF"
                     /locus_tag="ACHLCY_000183"
     CDS             complement(91052..92632)
                     /gene="hyfF"
                     /locus_tag="ACHLCY_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP0367515.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(92637..93287)
                     /gene="hyfE"
                     /locus_tag="ACHLCY_000184"
     CDS             complement(92637..93287)
                     /gene="hyfE"
                     /locus_tag="ACHLCY_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP0367516.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(93299..94738)
                     /gene="hyfD"
                     /locus_tag="ACHLCY_000185"
     CDS             complement(93299..94738)
                     /gene="hyfD"
                     /locus_tag="ACHLCY_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFP0367517.1"
                     /translation="MENLALTTLLLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLLFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFALSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASKVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(94755..95702)
                     /gene="hyfC"
                     /locus_tag="ACHLCY_000186"
     CDS             complement(94755..95702)
                     /gene="hyfC"
                     /locus_tag="ACHLCY_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP0367518.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(95713..97731)
                     /gene="hyfB"
                     /locus_tag="ACHLCY_000187"
     CDS             complement(95713..97731)
                     /gene="hyfB"
                     /locus_tag="ACHLCY_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP0367519.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGFAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(97731..98348)
                     /gene="hyfA"
                     /locus_tag="ACHLCY_000188"
     CDS             complement(97731..98348)
                     /gene="hyfA"
                     /locus_tag="ACHLCY_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP0367520.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(98601..99071)
                     /gene="bcp"
                     /locus_tag="ACHLCY_000189"
     CDS             complement(98601..99071)
                     /gene="bcp"
                     /locus_tag="ACHLCY_000189"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP0367521.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(99071..99661)
                     /gene="gcvR"
                     /locus_tag="ACHLCY_000190"
     CDS             complement(99071..99661)
                     /gene="gcvR"
                     /locus_tag="ACHLCY_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP0367522.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPRPRPRPPMPASV
                     WVQVDVADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASAD
                     AANIEQAFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            99807..100685
                     /gene="dapA"
                     /locus_tag="ACHLCY_000191"
     CDS             99807..100685
                     /gene="dapA"
                     /locus_tag="ACHLCY_000191"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP0367523.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            100702..101736
                     /gene="bamC"
                     /locus_tag="ACHLCY_000192"
     CDS             100702..101736
                     /gene="bamC"
                     /locus_tag="ACHLCY_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP0367524.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            101949..102662
                     /gene="purC"
                     /locus_tag="ACHLCY_000193"
     CDS             101949..102662
                     /gene="purC"
                     /locus_tag="ACHLCY_000193"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP0367525.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            102816..103043
                     /gene="vapB"
                     /locus_tag="ACHLCY_000194"
     CDS             102816..103043
                     /gene="vapB"
                     /locus_tag="ACHLCY_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFP0367526.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            103043..103441
                     /locus_tag="ACHLCY_000195"
     CDS             103043..103441
                     /locus_tag="ACHLCY_000195"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0367527.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            103588..104451
                     /gene="ypfJ"
                     /locus_tag="ACHLCY_000196"
     CDS             103588..104451
                     /gene="ypfJ"
                     /locus_tag="ACHLCY_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP0367528.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            104466..106481
                     /gene="tmcA"
                     /locus_tag="ACHLCY_000197"
     CDS             104466..106481
                     /gene="tmcA"
                     /locus_tag="ACHLCY_000197"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP0367529.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            106555..107253
                     /gene="ypfH"
                     /locus_tag="ACHLCY_000198"
     CDS             106555..107253
                     /gene="ypfH"
                     /locus_tag="ACHLCY_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0367530.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(107363..107563)
                     /gene="ypfN"
                     /locus_tag="ACHLCY_000199"
     CDS             complement(107363..107563)
                     /gene="ypfN"
                     /locus_tag="ACHLCY_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP0367531.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(107591..108718)
                     /gene="dapE"
                     /locus_tag="ACHLCY_000200"
     CDS             complement(107591..108718)
                     /gene="dapE"
                     /locus_tag="ACHLCY_000200"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP0367532.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(108722..109078)
                     /gene="yffB"
                     /locus_tag="ACHLCY_000201"
     CDS             complement(108722..109078)
                     /gene="yffB"
                     /locus_tag="ACHLCY_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP0367533.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT        26033 a        27655 c        29577 g        25890 t
ORIGIN      
        1 gaatgattat gctaatgtca tcaattaaat aaatataatg gcgttaaggc ttcccagtaa
       61 tataattaat actctacttc cagagtagaa tattaaattt tatccgcgtg gtgcatcagc
      121 acaaatttat cccacaactg ttcttctgtc tcgacatgcg ccggatcttt cacaatagta
      181 ttggggatcg ggcacacctt ctggcaggtt ggtgtctcgt agtgccctac gcattcggta
      241 cacttatcgc tgttaatctc gtagatatga tctcccattg aaatcgcctc attcgggcat
      301 tcgggttcac acatatcaca attgatgcat tttttagtga ttaacaacgc cataaggaaa
      361 ctctcaaaac aacacaaacc gggcgggcat tatacgcgca ttctttactc agaccagttt
      421 tttcaccagc gcctcactat gacgaatacg ttctggtgcc agttccagat cctgctgaat
      481 cagcgcaatg aacagcaaat ccgttaacat tccctgagcg tgacaagcag agattgaagc
      541 gctgtttgtc gcttgttctt cggcaatggt atacaggcaa tgagaagcac gctgttgcag
      601 ggcattcgga gtaaagccgg taatcgccag cacttttccg cccactcgca gcatctcatc
      661 tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa atggctaata acagatcgtc
      721 aggggacgac gcctgtactg ttgcgagtag cgcatgcata tcgcgcactg cggcagcatt
      781 gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc accagacccg aagcgccaat
      841 accggtcaga attatccgcc gcgcagagcg caacattgtt acgcattcat gcagtttctc
      901 ttcactatta acgtttagcg ttgcgtacat agcggcagta ttttctttaa tcagtttttc
      961 gccgaccagc cgtaacggat catcaccgcg gatttggtta tgaatgggca cggagggcga
     1021 ttccggctgg cttgccagcg cttcactcaa cgccaactta agcgccggaa aacctttata
     1081 gccgagtttt tgcgcgaact tcacgacgct ggactgactg actccggctt cgttggccag
     1141 ttgctgagag cttaaatggc gcgccgtatc aggttgtagc agcagataat ccgccagttt
     1201 tttatcactc tgggcaagcc cctggtaacg ctgacggata cgaagtaagc cgttcatatc
     1261 tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga gagacacgcg agtgaatttt
     1321 atattccatt gccgctaatt gccatgaatt aaaaattcca gttagccatg atgcggtcga
     1381 ttgttgccgg aaaagtgatg atctatctgt taggctatgt agtagcgaaa attaatcagg
     1441 atgtttcagt ccagaggagt atggtttggc aactcacgag cgtcgtgtgg tgttttttga
     1501 cttagatgga acattgcatc agcaggatat gttcggcagt tttctgcgct atttactgcg
     1561 tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta ccgattatag ccattgcgtt
     1621 attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg cttctgtggg ggtgcacttt
     1681 tggtcacagc gaagcacgtt tacagacgtt gcaggccgat ttcgtgcgct ggtttcgcga
     1741 caatgttacc gcctttccgc tggttcagga gcgattaacc acctacctgt taagttccga
     1801 tgctgatatc tggttgatta ccggctctcc gcagccgctg gttgaagcgg tttatttcga
     1861 tacgccctgg ctgccgcggg ttaatcttat cgccagccaa attcagcgtg gctatggtgg
     1921 ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc gcacaactgg agcgcaaaat
     1981 cggcactccg ctgcggctgt acagtggcta tagcgacagt aatcaggaca atccgctgct
     2041 ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt gaactccagc aactggaata
     2101 gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt ataaccggag ttttcttttt
     2161 gtctgaagtc gaatttagcc acgaatactg gatgcgtcac gcgctgacgc tggcgaaacg
     2221 tgcctgggat gagcgggaag tgccggtcgg cgcggtatta gtgcataaca atcgggtaat
     2281 cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc accgcacatg cagaaatcat
     2341 ggccctgcgg cagggtggtc tggtgatgca aaattatcgt ctgatcgacg ccacgttgta
     2401 tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg atccacagtc gcattggtcg
     2461 cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg ggatctttaa tggatgtgct
     2521 gcatcatccg ggtatgaatc accgagtgga aattacggaa ggaatactgg cggatgagtg
     2581 cgcggcgttg ctcagtgact tctttcgcat gcgccgccag gaaattaaag cgcagaaaaa
     2641 agcgcaatcc tcgacggatt aattttgttt ctcttcactc cctttcctgg aaaacagcag
     2701 agagggagaa tgggtcaaat agtttgatga cgactgggaa agaaacgaga gaaaaggaaa
     2761 tttctctttg cccaactccg taggcgatac cgccggataa tcctgcgcca gttgcatcgc
     2821 cgcttctgta gcctgcttct ctttctcttg cagataaccc accaggctaa tctgatactt
     2881 acgaatattt tcgacataag cgtaggcttc atgtccacga gcgtagccgt aagtcagctt
     2941 gctgtaatag ggtttctggc taagtaaagg cagacgctgt tttacgtcag cccaactgtc
     3001 aggattccct ttggtttttg ccgtcagggc gcgggcatcc agcatatgcg catagcccat
     3061 attgtacgca gcgagggcaa accagatccg ctcgttctcc ggcacacttt ccggcacttt
     3121 actcatcata tcctgcaaat aacgcacgcc accgctgatg ctctgttcgg catcggtacg
     3181 atccgtaatg ccgaggcttt gcgcggtatt tttggttaac atcatcatgc cgcgcacacc
     3241 cgtcggtgaa gtggcctgtg catcccagtg cgattcctga taagcaatag cggccagcaa
     3301 acgccagtca atttcttcgg cgtatttctc aaacaggggc tttaactgcg gcagtaccgc
     3361 atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca aaatcatcgc catgccccag
     3421 gtatttctct tcaatgcgtg ccagcgtacc gtcttcattc atttcgttga agaagtcgag
     3481 cagggcggcg gaaagggtat tatcgccatc taacgggcta aaccaagtca ccggttgttc
     3541 atcggtgata tcgagcgcta cggcgagctc cgggtgaacg cgctgaaaca ggctgatggc
     3601 gacagaatca gcaatggtgt aatcgagttt tccttcgatg acatcttcca ttaattccgc
     3661 agagcctttt ttgtcgtcta ccttccagct taattccggg aattttgttt ctttcagggt
     3721 ctggagatcg ttaaccacca catgacccgg tgcaacggtg agttgctccg ccgtcaggtt
     3781 gcccagcgta cgtgggcgat actgacccac tttataaacc agttgttgtg acacggaata
     3841 ataggtaggg ccaggctgat aattttttac ccgctcactg ttatagacca gtcctgccgc
     3901 cagcaggtcg gcgttaccat tatcaaggtc gtcaaacagc tggctgatat tctgccgcac
     3961 ggtcactttc agttttacgc cgaggtaatc ggcaaactgt ttcgccagtt cgtaatccag
     4021 gccaaaaggt ttcccgttga tttcgttata agtcagggga gtatgaatgg tgctcacacg
     4081 caactctccc cgcgcttgaa tggcggcgat acggttgtcg gctttaccaa accagggaat
     4141 ggatggccag agagcgaccg cgagcagcag tgccagaatg ccgatgaaca gataattaat
     4201 ctttaatttt ttcaattagt taattctctg tgtcgtgcgc gtcccagctt gaaaaaacgt
     4261 aataatagtg aaaggtttac tcttaaatga gcggcatttt gcgtaaacct gcgccagatg
     4321 gcaacttatt acagccattg gcggcacgcg ttgctaattc acgatggtga ttttatttcc
     4381 acgcaaacgg tttcgtcagc gcatcagatt ctttataatg acgcccgttt cccccccttg
     4441 ggtacaccga aagcttagaa gacgagagac ttatgatgga aattctgcgt ggttcgcctg
     4501 cactgtcggc attccgaatc aacaaactgc tggcacgttt tcaggctgcc aggctcccgg
     4561 ttcacaatat ttacgccgag tatgtccatt ttgctgacct caatgcgccg ttaaacgatg
     4621 atgagcacgc acaacttgaa cgcctgctga aatatggccc ggcactcgcc agccacgccc
     4681 cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac catctctccc tggtcttcga
     4741 aagcgaccga tattgcccat aactgcgggc tacaacaggt aaaccgcctt gagcgcggcg
     4801 ttgcttacta tatagaagcc ggtacgctga ccaatgaaca atggcagcag gttaccgctg
     4861 aactgcacga ccgcatgatg gaaacggtct tttttgcttt agatgatgca gagcagctgt
     4921 ttgcacacca tcaaccgact ccggtcacca gcgttgattt gctggggcag ggccgtcagg
     4981 cgctaatcga cgctaacctg cgtcttggcc tggcactggc ggaagatgaa attgactatc
     5041 tgcaggatgc tttcacaaag cttggtcgta acccgaacga catcgagctg tatatgttcg
     5101 cccaggcgaa ctccgagcac tgccgccaca agatttttaa cgccgattgg gttatcgacg
     5161 gtgagcaaca gccgaaatcg ctgtttaaga tgatcaaaaa caccttcgaa accacgccag
     5221 attacgttct ctctgcttat aaagataacg ccgccgtaat ggaaggttct gaagtgggcc
     5281 gctactttgc tgaccacgaa acgggccgct acgatttcca tcaggaaccg gcgcatattc
     5341 tgatgaaagt cgaaactcac aaccacccga cggcgatttc tccgtggccg ggcgcggcga
     5401 ccggttccgg cggtgaaatc cgcgatgaag gtgccaccgg gcgcggcgcg aagccgaaag
     5461 cgggtttggt tggtttctcc gtttccaacc tgcgaattcc tggcttcgaa cagccgtggg
     5521 aagaagattt cggtaagcct gagcgcattg tcaccgcgct ggacatcatg accgaaggcc
     5581 cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc ggcactgaat ggctacttcc
     5641 gtacttatga agaaaaagtg aacagccaca acggcgaaga gctgcgcggt tatcacaaac
     5701 cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga tcacgtacaa aaaggcgaga
     5761 tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc aatgaacatc ggtcttggcg
     5821 gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc cgacctcgac tttgcttccg
     5881 tacagcgcga caacccggag atggagcgtc gttgccagga agtgatcgac cgttgctggc
     5941 agcttggtga tgctaaccca attctgttta tccacgacgt cggtgcgggc ggtctttcta
     6001 acgcgatgcc ggagctggtg agcgacggcg gacgcggcgg taaatttgaa ctgcgcgata
     6061 ttctaagcga cgaaccgggc atgagcccgc tggaaatctg gtgtaacgaa tcccaggaac
     6121 gctacgtgct ggcggttgct gccgatcaat taccgctgtt tgacgaactg tgtaagcgtg
     6181 agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga actgtatctt tctctgcacg
     6241 atcgtcattt tgataatcag ccgatcgatc tgccgctgga cgtcctgctt ggtaaaacgc
     6301 cgaagatgac ccgcgatgta caaacgctga aagcgaaggg cgatgcgctg gtccgtgaag
     6361 ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct gccgaccgtg gcggagaaaa
     6421 ctttcctggt gaccatcggt gaccgcagcg taactggcat ggtagcgcgc gatcagatgg
     6481 tgggtccgtg gcaggtgccg gtcgctaact gcgcggtcac taccgccagc ctcgacagct
     6541 actacggtga agcgatggcg attggcgagc gtgcgccggt tgcgctgctg gatttcgccg
     6601 cctctgcccg tctggcggtc ggtgaagcgt taaccaatat tgccgcaaca cagattggcg
     6661 atatcaaacg cattaaactt tccgccaact ggatggcggc ggcaggccac cctggtgaag
     6721 atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga gctttgtccg gcgctgggcc
     6781 tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac ccgctggcag gaaggtaacg
     6841 aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc tgcatttgcc cgcgtggaag
     6901 atgtacgtca caccatcacg ccgcagcttt ctaccgaaga taacgcactg ctgctgattg
     6961 atttgggcaa aggcaataac gcgctgggcg caacggcgct ggcgcaggtt tatcgtcagc
     7021 ttggcgacaa accggcagat gtacgcgatg tcgcgcaact gaaaggcttc tatgacgcga
     7081 ttcaggcgct ggttgcacag cgtaagctgc tggcgtatca cgaccgctct gatggcggtc
     7141 tgttggtcac actggcggaa atggcctttg ctggtcattg tggcattgat gcggatatcg
     7201 gctctctggg tgacgatcgc ctggcggcgt tgtttaacga agaactgggt gcggtgattc
     7261 aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact ggcacagcat gggcttgctg
     7321 attgcgtcca ttatgttggg caggcggttt ccggtgaccg ttttgtgatt accgccaacg
     7381 ggcagactgt attcagcgaa agccgcacta cgttgcgtgt ctggtgggca gaaactacct
     7441 ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga ccaggagcac caggcgaaat
     7501 caaacgacgc cgatccgggc ctgaatgtaa aactgtcgtt cgatatcaac gaagatgtgg
     7561 cagcaccgtt tattgccact ggcgcgcgcc cgaaagttgc cgtactgcgt gagcagggcg
     7621 tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc aggctttgat gctatcgacg
     7681 tgcatatgag tgacctgctg gctggacgca cgggcctgga agatttccac gccctggtcg
     7741 cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg tgaaggttgg gcgaagtcaa
     7801 tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt cttccaccgt ccgcaaacgc
     7861 tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa tctgcgtgaa ctgatcccag
     7921 gtagtgagtt gtggccacgt tttgtgcgca atacctccga tcgctttgaa gcgcgtttca
     7981 gcctggttga agtgacccaa agcccgtcgc tgctgttgca ggggatggtg ggctcgcaaa
     8041 tgccgattgc tgtctctcac ggtgaagggc gcgtggaagt gcgtgatgcg gcgcatctgg
     8101 cggcactgga aagcaaaggg ctggtggcac tgcgctatgt cgataacttc ggcaaagtca
     8161 ctgaaaccta cccggctaac ccgaacggtt ctccgaacgg tattacgtcg gttacgactg
     8221 aaagtggtcg agtcaccatt atgatgccgc acccggaacg tgttttccgt actgtcagca
     8281 actcctggca tccggaaaac tggggcgagg atggcccatg gatgcgcatt ttccgcaatg
     8341 cgcgtaagca gttggggtaa gtcatcagtt cattggtttt gcagcccgga tgcggcattt
     8401 acgccgcatc cggcattaag caccaaccac tatcataagc tcccaactcc gggagctttt
     8461 ttgtgtctgt aaatcacgac aatgggtggt ttaccgtgtc gctttctggc gacacttaaa
     8521 tgattgattt tattgtaatc taataagttt atcttaagtt gtctcttttt agcgacacag
     8581 tggctgattt acattcaaat agcgaagaag ccataaatcc ctcaaagcaa cgtataatca
     8641 gtacgttacc aaactatttt ctttattggc acagttactg cataatagta agcagtggct
     8701 cattcaccga cttatgtcag ccccttcggg acgtgctaca taaaattcga atgacgcaca
     8761 acaaggtgcc tgccgtccaa cttctgatat cagcgtagct atatcaacca tcgggcgaaa
     8821 cgtcgagtta ggcaccgcct tattccataa caacgccggg taattcccgg ctttgttgta
     8881 tctgaacttc ccctcggtta gcatcaggct attcgcgtct gacgagagta acaccttgaa
     8941 acgctggccc gtttttcccc gctcattacg acaactggta atgctggcat ttttgctaat
     9001 tctactaccc ctgttggtgc tggcatggca agcctggcaa agcctgaatg cgcttagcga
     9061 tcaggcggcg ctggttaacc gcactacgct tattgatgcc cggcgtagtg aagcgatgac
     9121 caacgcggcg ctggagatgg agcgtagcta ccgtcagtat tgcgtgctgg acgaccctac
     9181 gcttgcgaag gtttatcaaa gccagcgcaa gcgttacagc gaaatgctcg atgcccacgc
     9241 aggcgtactg ccagacgata aactttacca ggcattgcgt caggatttga acaatctggc
     9301 tcaacttcag tgtaacaaca gtggtcccga tgctgccgcc gccgcgcgtc ttgaagcctt
     9361 tgccagtgcc aataccgaaa tggtgcaggc cacgcgcaca gtggtgttct ctcgtgggca
     9421 gcaacttcag cgtgaaatcg ccgaacgtgg gcaatatttt ggttggcaat cgctggtgct
     9481 atttctggtg agtctggtga tggtgctact tttcacgcgg atgattatcg ggccggtgaa
     9541 aaatatcgag cgcatgatca accgtctggg ggaagggcgt tctctgggca atagcgtttc
     9601 gttcagtgga ccgagcgagt tacgctcggt tgggcaacgt attctttggt taagtgagcg
     9661 cctgtcatgg ctggaatccc aacgccatca atttttaaga catttatctc atgaattaaa
     9721 aacgccactg gcgagtatgc gcgagggcac tgaattactg gctgaccagg ttgtcgggcc
     9781 gcttacgcca gagcaaaaag aggtggtgag cattcttgat agcagcagcc gcaatttgca
     9841 aaaactgatc gaacaactgc ttgattacaa ccgtaaacag gcggacagtg cggtggaact
     9901 ggagaatgtt gaattagcac cgctggtgga gacagtggtt tctgctcata gcctgcccgc
     9961 acgggctaaa atgatgcata ccgacgtcga tctcaaagca acagcttgcc tggcggagcc
    10021 aatgctgctg atgagcgtac tggataatct ttactccaat gcggtgcact acggggctga
    10081 atccggtaac atttgccttc gcagcagctt acatggtgca cgggtttata ttgatgtcat
    10141 caatacaggc acacccattc cgcaagagga acgcgccatg atcttcgaac ccttttttca
    10201 aggaagccac cagcgaaaag gggcggtgaa gggcagcggt ctgggattaa gcattgccag
    10261 ggattgtatt cgccgtatgc aaggggaact gtatctggtc gacgagagcg ggcaagacgt
    10321 ttgtttccgc attgaattgc cgtcgtcgaa aaacacgaaa taatgctaac agcctatccc
    10381 atcaggctat tttatttgcc agttcggccc cgggcagtgc tcgaaatcct cacgtacgac
    10441 gtgtacgctc cggtttctcc gcgctgtcca tgtccaaact gcctgcaata attacgccca
    10501 ctgggatagg cattaagtct ggtgaatatg cgacacattt ttcaacgatt attgccccga
    10561 cggctgtggc tggcagggct gccatgtctg gcattgctgg gttgcgtgca gagtcacaat
    10621 aagccagcca ttgatacgcc agcagaagaa aaaattccgg tttatcaact ggctgactat
    10681 ctttctactg aatgtagcga catctgggcg ctgcaaggta aatcaaccga aaccaatccg
    10741 ctttactggc tgcgggcgat ggattgtgct gatcgtttaa tgcctgcgca gtcacgtcag
    10801 caagctcgcc aatatgacga cggcagctgg caaaatacct ttaaacaagg gattttgctc
    10861 gccgacgcca aaattacgcc gtatgagcgc cgccagctgg tcgcgcgcat tgatgcgcta
    10921 agtaccgaga tcccggcaca ggttcgccct ctgtatcaac tctggcgcga cggtcaggcg
    10981 ctgcaactgc aactggcgga agagcgccaa cgctacggca agctccagca ggccagcgac
    11041 agcgagctgg atacattgcg ccagcaacat cacgttctac agcagcaact ggaactcacc
    11101 acccgcaagc tggaaaacct gactgatatt gaacgccagc tctcgacccg caaaccggct
    11161 ggaaatttct cgccggatac gccgcacgag ggtgaaaagc ccgcgccatc cacccatgag
    11221 gtcactcctg atgagccata aacctgcgca tttattattg gtcgatgacg atccgggatt
    11281 gctgaaactg cttggcctgc gcctgaccag cgaaggctac agcgtggtca cggcggaaag
    11341 tggcgctgaa ggattacggg tactgaatcg cgaaaaagta gatttagtca tcagcgacct
    11401 gcggatggat gaaatggacg gtatgcagct gtttgctgaa attcagaaag tgcagccggg
    11461 aatgccagta attattctta ccgcgcatgg ttctattccc gatgccgttg ctgcaacaca
    11521 gcagggcgtg ttcagtttcc tcaccaagcc tgtcgataaa gatgcgctat atcaggcaat
    11581 tgacgatgcg ctggaacaat ccgcgccagc caccgatgaa cgctggcgcg aggcgattgt
    11641 cacccgcagc ccgctgatgc tgcgtttgct ggaacaggcg cggctggtgg cgcaatcaga
    11701 cgttagcgtt ttgattaacg gccagagcgg caccgggaaa gagattttcg cccaggctat
    11761 ccacaacgcc agcccgcgca atagcaaacc atttattgct attaactgtg gcgcattgcc
    11821 cgagcaattg ctggagtcgg agctgtttgg tcatgcgcgt ggcgcgttta ctggcgctgt
    11881 cagcaatcgc gaaggtttat tccaggcggc ggaaggcggt acgctatttc tcgatgagat
    11941 tggcgatatg cccgcaccgt tgcaggtcaa actgctgcgc gtgttgcagg agcgtaaagt
    12001 gcgcccgctg ggcagtaacc gcgatattga tatcgacgtg cggattattt ccgccactca
    12061 ccgcgacctg ccaaaagcga tggcgcgcgg ggaattccgt gaagacctct attaccgcct
    12121 caacgttgtc agcctgaaaa ttccggctct ggcagagcgc acagaagaca ttccgcttct
    12181 ggcaaatcac ctgttgcgcc aggcggcaga gcgacataaa ccgtttgtcc gcgcgttctc
    12241 cactgatgcg atgaaacgcc tgatgaccgc tagctggccg ggtaatgtgc gccagttggt
    12301 caacgtgatt gaacagtgcg tggcgctgac ctcatctccg gttattagcg atgcattagt
    12361 agagcaggcg ctggagggtg aaaatacggc gctgccaacc tttgttgagg cacgtaatca
    12421 gtttgaactc aactatttgc gtaagctgct gcaaatcacc aaaggcaacg tcacacacgc
    12481 ggcgagaatg gcggggcgca accggacaga attttataaa ctgctttccc gacacgagct
    12541 ggatgcaaac gatttcaagg aatgaattgg cgttatgtgt tacgtttagc agatcaaaag
    12601 acaggcgacc ttttcaagga atagcatgaa aaagattgat gcgattatta aacccttcaa
    12661 gctggacgat gttcgcgaag cactggctga agtcggcatt accggcatga cggtcactga
    12721 ggtgaaaggt tttggccgcc agaaaggcca taccgagctg taccgcggcg cggagtatat
    12781 ggtggatttt ctgccgaaag tgaaaattga gattgtcgta ccggacgaca ttgtcgatac
    12841 ctgtgttgat accattattc gcacggcgca aaccggcaaa atcggtgacg gtaaaatctt
    12901 cgtcttcgac gtggcacggg tcattcgcat ccgtaccggt gaagaggacg acgcggcaat
    12961 ttaaaccgcg tcaagggttg ccggatgttt ccatccggca acatcaaatt acagcacctt
    13021 atgcgggcca aagcattcgt aatgaatgtt ttcctgcttc acgcccagat ccactaactg
    13081 tttcgcggta aactgcatga agccaaccgg gccgcagaga tagaactgca ttgtcggatc
    13141 gctgaacgca ccttccagtt tgctcaaatc catcagacct tcgctatcaa actgaccttt
    13201 agcgcgatcg gcttcgctcg gctgacgata ccaggtgtgc gcggtaaagc gcggcagtga
    13261 ctgccccagt tccttaactt catcagcaaa ggcgtgaaca tcgccatttt ctgccgcatg
    13321 gaaccagttc acttgtgctg tgtggcctgc ttttgccagc gtgtcgagca ttgccagcat
    13381 tggcgtttga ccaacaccgg cagagattaa cgtcactggt gtgtcatctg cgacagccat
    13441 aaagaaatca cctgccggag cgaccagttt cacgacatcg ccaacattgg cgtgattgtg
    13501 caaccagttg gatacctgcc caccctcttc gcgtttcacc gcaatacgat agcctttgcc
    13561 atccggtttg cgagtcaaag agtactgacg aatttcctga tgcgggaaac cttccggctt
    13621 cagccagacg ccgagatatt gccccggacg gtattctgcc actgcgccac cgtcgaccgg
    13681 ctccagttcg aagctggtga taagcgcgct gcgcggtgtt ttagccacaa tgcggaaatc
    13741 gcgagtacct tcccaaccac cggctttgct ggcgttttcg ttatagattt ccgcctcgcg
    13801 attgataaat acattagcca gtacaccata ggctttaccc cacgcgtcca gcacttcctg
    13861 ccccgggctg aacatttcgt ccagcgttgc caacaggtgt tcaccgacga tgttgtactg
    13921 ttccggtttg atctggaagc tggtgtgctt ctgcgcgatt ttttctaccg ctggcagcag
    13981 cgcaggcagg ttttcaatat tactggcgta ggcggcaata gcgttaaaca gggcttcacg
    14041 ttgatcgcca ttacgctggt tactcatgtt aaaaatttct ttgagttctg ggttatgagt
    14101 aaacatacgg tcgtagaaat gggcggttaa ctttggcccc gtttccacca gtaaagggat
    14161 ggtggctttt actgtagcga tggtttgagc gtcaagcata tggtcttcct ttttttgcat
    14221 cttaattgat gtatctcaaa tgcatcttat aaaaaatagc cctgcaatgt aaatggttct
    14281 ttggtgtttt tcagaaagaa tgtgatgaag tgaaaaattt gcatcacaaa cctgaaaaga
    14341 aatccgtttc cggttgcaag ctctttattc tccaaagcct tgcgtagcct gaaggtaatc
    14401 gtttgcgtaa attcctttgt caagacctgt tatcgcacaa tgattcggtt atactgttcg
    14461 ccgttgtcca acaggaccgc ctataaaggc caaaaatttt attgttagct gagtcaggag
    14521 atgcggatgt taaagcgtga aatgaacatt gccgattatg atgccgaact gtggcaggct
    14581 atggagcagg aaaaagtacg tcaggaagag cacatcgaac tgatcgcctc cgaaaactac
    14641 accagcccgc gcgtaatgca ggcgcagggt tctcagctga ccaacaaata tgctgaaggt
    14701 tatccgggca aacgctacta cggcggttgc gagtatgttg atatcgttga acaactggcg
    14761 atcgatcgtg cgaaagaact gttcggcgct gactacgcta acgtccagcc gcactccggc
    14821 tcccaggcta actttgcggt ctacaccgcg ctgctggaac caggtgatac cgttctgggt
    14881 atgaacctgg cgcatggcgg tcacctgact cacggttctc cggttaactt ctccggtaaa
    14941 ctgtacaaca tcgttcctta cggtatcgat gctaccggtc atatcgacta cgccgatctg
    15001 gaaaaacaag ccaaagaaca caagccgaaa atgattatcg gtggtttctc tgcatattcc
    15061 ggcgtggtgg actgggcgaa aatgcgtgaa atcgctgaca gcatcggtgc ttacctgttc
    15121 gttgatatgg cgcacgttgc gggcctggtt gctgctggcg tctacccgaa cccggttcct
    15181 catgctcacg ttgttactac caccactcac aaaaccctgg cgggtccgcg cggcggcctg
    15241 atcttggcga aaggtggtag cgaagagctg tacaaaaaac tgaactctgc cgttttccct
    15301 ggtggtcagg gcggtccgtt gatgcacgta atcgccggta aagcggttgc tctgaaagaa
    15361 gcgatggagc ctgagttcaa aacttaccag cagcaggtcg cgaaaaacgc taaagcgatg
    15421 gtagaagtgt tcctcgagcg cggctacaaa gtggtttccg gcggcactga taaccacctg
    15481 ttcctggttg atctggttga taaaaacctg accggtaaag aagcagacgc cgctctgggc
    15541 cgtgctaaca tcaccgtcaa caaaaacagc gtaccgaacg atccgaagag cccgtttgtg
    15601 acctccggta ttcgcgtggg tactccggca attacgcgtc gcggcttcaa agaagcagaa
    15661 gcgaaagaac tggctggctg gatgtgtgac gtgctggaca gcatcaatga tgaagctgtt
    15721 atcgagcgca tcaaaggtaa agttctcgac atctgcgcac gttatccggt ttacgcataa
    15781 gcgaaacggt taatctgttg aaaaaaggct tccttcggga agcctttcta cgttattgcg
    15841 ccatcaaatc tgccgtaact gcgcctcaac atacaaatag ccaattccca gcacctgttg
    15901 tgcgcggctt aattgcccga agccaatttg cgtcgctttc acgctcggtc cttcgtcgcg
    15961 gtcgaacggg ttaaatcctg tctggcgaat aatagtatta agccagttct caccaaaggc
    16021 acaactgcga ccgtacaacc aaatctgatt aatattgagg atgttgagga agttatacag
    16081 actcaaacca atggcattgg cactgcggtc aacccagctg gtgatccacg gttctccact
    16141 ttgccaggca gcgattaact gcgctgtagt cagtttttca ggatcaagtt gagtactaac
    16201 cggttgtgat tttagccata cccgcgcctg tttttttaat gcgcttaacg aggcgacggt
    16261 ttccaggcag ccataacgtc cacagtcgca gacgacgcca tccggattaa cgatggtgtg
    16321 accaatctgt ccgctaccat acaaactgcc gcgataaatt tgctcgttaa tcacgaacga
    16381 cgagccaatg ccgtaatcaa cgttgatcac gcagaaatcc cgttcctgcg aattattttg
    16441 ccatttctcc gccagcgcca gcatcacgca gtcattatcg accatcaccc gaatgccgag
    16501 cttctcttcc agcagatatt tcacctccac cggcgttgtc cacggcgctt gcggcatggt
    16561 ttgcgacacg ccggtcacag gatcaacctg accgtggatt gccagcgcca gattgatggt
    16621 gcggtccggc cacaatttac ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc
    16681 ctgcggcgtc ggtgcatcaa tctgtaaata ctcaaattca cctttcggac ttaaacaagc
    16741 attagcaacc tgacactcaa tactggtggg cgtcacgttc aggcacagcg tccagtcacc
    16801 ttccggcgca atcagccatg taccgctact atgcccgcgc gtctggcttt catcgtcaat
    16861 attcaccacc cgtttttcgc tttccagttc ctgcaaaata ttactgactg ccggaatcga
    16921 aatttgcgcc agccgggcca gcgttgattt attggcgcgc ttttgccggt acagcagttc
    16981 cagaatcacg catttgttat ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt
    17041 cactaaacct tgtttagtta attgcgtgat tattccgcga aaacattgcc agattgttca
    17101 atacactgcc acaaatcttt taattcagta tgtcttgtta atattgaggg caccatgact
    17161 ccagtaaaag tgtggcaaga gcgcgttgag atcccgacct atgaaaccgg gccgcaggat
    17221 atacatccca tgttcctcga aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc
    17281 tacggcgtga ccgatacgct gagcgagcag aaaaccctga aatcctggca ggcggtgtgg
    17341 ctggaaaacg actacatcaa agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc
    17401 gcgtgggata aagtgaaaca gcgcgatttt gtttatcaca atgaagtcat taaacctgcg
    17461 ctggtggggc tgctggggcc gtggatttcc ggtgggattg agtttaactg gccgcaacat
    17521 catcgcccga ccacctttat gcccgttgat ttcaccctcg aagcccatga agacggtgca
    17581 cagacggtgt gggtaggcga aacggagccg atgcatggtt tacaggtgat gacaggtttc
    17641 accctgcgcc ctgaccgggc ggcgctggaa atcgccagcc gcgtctataa cggtaacgcc
    17701 acgccgcgtc atttcttgtg gtgggccaac ccggcagtga aaggggggga agggcatcag
    17761 agcgttttcc caccggatgt gactgcggtg tttgatcacg gcaaacgggc cgtctccgct
    17821 ttccccatcg ccaccggcac ttactacaaa gtggactact ccgccggagt ggacatttct
    17881 cgctataaaa atgtgcctgt tccaacctca tatatggccg aaaaatcgca gtacgatttt
    17941 gtcggcgcgt ggtgtcacga tgaagatggc ggtttgctgc acgttgccaa ccaccatatt
    18001 gcgccaggta aaaaacagtg gagttgggga cacagtgaat ttggccaggc gtgggataag
    18061 agtctgaccg acaataacgg tccgtatatc gaactgatga ccggtatttt tgccgataac
    18121 cagcccgatt ttacctagct tgatgcttac gaagagaagc gttttgagca gtatttcctg
    18181 ccttatcatt ctttgggcat ggtgcaaaat gcctcccgcg atgcggtgat aaaactccag
    18241 cgtagtaagc gggggattga gtgggggctg tatgccatct ctccgttgaa cggataccgc
    18301 ctggcgatcc gcgaaatcgg caaatgcaac gcgttacttg atgatgccgt ggcactgatg
    18361 cctgcgaccg ccatccaggg cgtgttgcac ggtatcaatc ctgaaaggct gaccattgag
    18421 ctctccgatg ccgacggcaa tattgtactg agttatcagg aacatcagcc gcaagagttg
    18481 ccgttgccgg acgtcgccaa agcgccactg gcagcacaag acattaccag tacagatgaa
    18541 gcctggttta tcggtcagca tctggagcaa tatcatcacg ccagccgttc accgttcgat
    18601 tactacctgc gcggcgtggc gctggatccg ctggattacc gctgtaacct ggcgctggcg
    18661 atgctggaat ataaccgcgc cgatttcccg caagcggtgg cgtatgccac tcaggctctg
    18721 aaacgcgcac atgcgctgaa caaaaatccg cagtgcggac aggcgagttt gattcgcgcc
    18781 agtgcttacg aacgtcaggg acaatatcaa caagccgaag aggatttctg gcgtgcggtc
    18841 tggagcggca acagcaaagc cggtggctat tatggcctgg cacgactggc ggcgcgtaat
    18901 ggtaacttcg acgctggtct ggatttttgc caacaaagtc ttcgcgcctg cccaaccaat
    18961 caggaagtgc tttgcctgca taatctgctg ctggtgttaa gtggtcgtca ggacaacgcg
    19021 cgtgtgcagc gcgagaaact gctgcgcgat tatccgctga acgccactct gtggtggctg
    19081 aactggttcg atggtcgtag cgaatcagct ctcgcgcagt ggcgcggtct gtgtcaggga
    19141 cgcgacgtta acgccctgat gaccgccggg caactgatta actggggaat gcccaccctg
    19201 gcggcagaga tgctgaacgc actggactgc cagcgcacgc tgccgcttta cctgcaagcc
    19261 agcttgctgc cgaaagccga acgtggcgaa ctggtcgcaa aagccattga tgtcttcccg
    19321 cagtttgtcc gtttcccgaa tacgctggaa gaagtggcgg cgctggagag tattgaagag
    19381 tgctggtttg ctcgccattt actggcctgc ttctactaca acaaacgtag ctacaacaaa
    19441 gccattgcct tatggcaacg ttgcgtagag atgtcgccgg agtttgccga cggctggcgc
    19501 gggttagcga tccatgcgtg gaataagcaa cacgattatg agctggccgc gcgttatctt
    19561 gataatgctt atcagcttgc gccgcaggat gcacgtctgc ttttcgaacg ggatttgctt
    19621 gataagttaa gtggagccac accggagaaa cgactggcgc gtctggaaaa taatcaggaa
    19681 attgcgctga aacgcgacga catgaccgca gaactgctca atttgtggca tctcacgggt
    19741 caggcagaca aagcggcgga cattctcgcc acgcgtaaat tccacccgtg ggaaggcggg
    19801 gaagggaagg tcaccagtca gtttatcctc aaccagttat tacgcgcctg gcagcatctt
    19861 gatgccagag agccgcagca ggccagcgaa ctgcttcatg ccgcgctgca ttatccggag
    19921 aatttaagcg aaggccgttt accggggcaa actgataacg acatctggtt ctggcaggcg
    19981 atatgcgcca acgcgcaggg cgatgaaact gaagcgatgc gttgtttacg tctggcggcg
    20041 accggcgatc gcaccattaa catccacagt tattacaacg atcagccggt tgattatctc
    20101 ttctggcaag gaatggcgct gcgactgctg ggtgaacagc aaaccgcaca gcaactgttt
    20161 agtgaaatga aacagtgggc gcaagagatg gcgaaaacca gtattgaggc ggatttcttt
    20221 gctgtttcac aacctgacct gttgtcgctg tatggcgatt tacaacagca gcataaagaa
    20281 aaatgcctga tggtggcgat gctggcgtcc gcgggactcg gggaggttgc gcaatatgaa
    20341 tctgctcgcg ctgaattgac ggcgattaat ccggcctggc cgaaagcggc attattcatc
    20401 accgtgatgc cttttatttt taactacgtt cactaaaccc tattaactgt aatatttgag
    20461 cggcacgcgc tgccgctcat cacaaaataa ttagaataag ccgttgttaa taaaaaccct
    20521 acaggaggca ctatgcctaa aaaaatgaga acaacccgta atctattatt aatggctacg
    20581 ctattaggta gcgcactatt tgccagggct gcggataaag aaatgaccat tggtgcaata
    20641 taccttgata cctagggata ttacgctgga gtgcgtcagg gcgttcagga tgcggcaaaa
    20701 gattcttcag tacaggtaca gttaattgaa actaacgccc agggtgatat ttcgaaagaa
    20761 agtacctttg ttgataccct cgtggcgcgt aatgtcgatg ccattatttt atcggcagtg
    20821 tctgaaaatg gcagtagccg taccgttcgt cgcgccagtg aagcgggtat tccggtgatt
    20881 tgctacaaca cctgtattaa tcaaaagggg ggcgataaat atgtctcggc gtatctggtc
    20941 ggcgatccgc tggaatttgg taaaaaactg ggtaacgctg ccgccgatta ttttattgcc
    21001 aataaaattg accagccgaa aattgctgtc atcaattgcg aagcctttga agtttgtgtg
    21061 cagcgacgta aaggatttga agaagtatta aaatcccgcg ttcccggcgc gcaaattgtc
    21121 gctaatcagg aagggactgt tttagataaa gcgatttccg ttggtgaaaa actgattatc
    21181 tccacgccgg atctcaacgc cattatgggg gagtcgggcg gtgcgacact cggcgcggta
    21241 aaagcggtac gtaatcaaaa tcaggccgga aaaattgctg ttttcggttc ggatatgaca
    21301 accgaaattg ctcaggagct ggaaaacaat caggtgctga aagcggtagt ggatatttcc
    21361 ggtaagaaaa tgggcaatgc tgttttcgcg caaacattga aggttatcaa taaacaagcc
    21421 gacggtgaaa aagtgattca ggtgccaatc gatctctata ccaaaacgga agatggtaaa
    21481 cagtggctgg caacgcacgt tgatggtctg ccctaatcgc tgattcgcag aggtccctat
    21541 gttcacggca acagaggcag tcccggtagc aaaagtggtg gcaggaaata agcgttaccc
    21601 cggcgtcgtt gcgctggata acgttaactt cacgctcaat aaaggcgaag ttcgtgcgct
    21661 gttaggtaaa aacggcgcgg gcaaatcgac tctcattcga atgcttaccg gcagcgaacg
    21721 cccggatagc ggtgatatct ggattggcga gacgcgactg gaaggtgacg aagctacgtt
    21781 gactcgccgt gccgctgaac tgggggttcg tgcggtttat caggagttaa gtctggtgga
    21841 gggactgacg gtggcggaaa acctctgcct cggtcagtgg ccccgccgca acggcatgat
    21901 tgattacctg caaatggcgc aggatgccca acgttgctta caggcgctgg gcgttgacgt
    21961 tagccctgaa caacttgttt caacgctaag cccggcgcaa aagcaactgg tggaaattgc
    22021 gcgggtgatg aagggcgagc cgcgcgtggt cattcttgat gaacctacca gttcgcttgc
    22081 gagtgcggaa gttgaactgg tgatcatcgc ggtgaaaaag atgtcggcac tgggcgtggc
    22141 ggtgatttat gtcagccacc ggatggaaga aattcgccgc attgcctcct gtgccaccgt
    22201 tatgcgcgat ggtcaggtgg cgggcgatgt gatgctcgaa aacacctcca cgcatcatat
    22261 tgtgtcgcta atgctagggc gtgatcacgt tgatattgcg ccggttgccc ctcaggtaat
    22321 tgtggatcag gccgtgctgg aagtccgtgc attacgccat aagcccaagc tggaggatat
    22381 cagctttacg ttacgtcgcg gcgaagtgct tggcattgct ggcctgctgg gggcagggcg
    22441 cagtgaattg ctgaaggcga ttgttgggct ggaggagtat gaacagggcg aaattgttat
    22501 caacggcgag aaaatcacgc gccccgatta cggcgacatg ctgaaacgcg gcattggcta
    22561 tacgccagaa aaccgcaaag aagcggggat cattccctgg ttgggcgttg acgaaaatac
    22621 agtgctgacc aatcggcaaa aaatcagcgc caacggcgta ctgcaatggt ccaccatccg
    22681 ccgcctgacc gaagaggtga tgcagcggat gacggtcaag gccgccagta gcgaaacgcc
    22741 catcggcacg ctttctggcg gcaatcagca aaaagtggtg atcggtcgtt gggtctatgc
    22801 cgccagccag attttgttgc tcgatgagcc aacgcgcggc gtcgatatcg aagccaaaca
    22861 gcagatttac cgtattgtcc gtgagctggc tgccgaagga aaaagcgtgg tgtttatctc
    22921 cagtgaagtg gaggagttgc cgctggtctg tgaccgcatc ctgttattac agcacggcac
    22981 gttctcgcag gagtttcacg ctccggtcaa tgtggatgag ctgatgtccg ccattctgtc
    23041 tgtgcactga tgaattttac cgaggaacct gaagatgtct gcttcgtcat taccattgcc
    23101 gcagggcaag agcgtctcgc tcaaacaatt tgtcagtcgc catattaatg agatcggttt
    23161 gctggtggtg attgccattc tttatctggt cttctccctg aacgcgccag gctttatctc
    23221 attgaataac cagatgaacg tgctgcgcga tgccgccacc attgggattg ccgcctgggc
    23281 gatgacgctg attattatct ccggtgaaat tgatgtcagc gttgggccga tggtggcttt
    23341 tgtctcggtg tgcctggcat ttttgctgca atttgaagtt ccgctggcgg tggcgtgtct
    23401 gctggtgttg ctgttaggcg cgctgatggg aacgctcgcc ggggtgctgc gcggcgtgtt
    23461 taacgtacca agtttcgttg ccacactggg gctgtggagc gccctgcgcg gaatggggct
    23521 gtttatgacc aacgcactgc cagtgccgat tgacgaaaac gaggtgctgg actggctggg
    23581 cggacaattt ctcggtgtgc cagtatccgc gctgatcatg atggtgttgt ttgcgctgtt
    23641 tgtgttcatt agccgaaaaa ccgccttcgg gcgctcggtt tttgccgttg gcggtaatgc
    23701 cacggcggcg cagttgtgcg gtattaacgt tcgtcgagtg cgcattctta tctttaccct
    23761 ttcgggatta ttagcggcgg tgaccggcat tttgctggcg gcacgcctcg gttccggtaa
    23821 cgcaggtgcc gcaaacggtc tggagtttga cgtcatcgcc gcggtcgtgg tcggcggtac
    23881 ggcactttcc ggcggtcgcg gttccctgtt cggtacattg cttggtgtgc tggtgattac
    23941 gctaatcggt aacggtctgg tgctgctcgg tattaactcc tttttccagc aggtggtacg
    24001 cggcgtcatc atcgtggtgg cggtgctggc gaatatcttg ctgacccagc gaagcagtaa
    24061 agcgaaacgc taaaccttaa cgcccctggc gcgggtgcgg cagggatgaa aattaatcgt
    24121 attgttaatg agggatttat gaaaacgatg ctggcagctt atttaccagg aaattcaacc
    24181 gtcgatctgc gggaagttgc ggtgccgacg ccggggatta accaggtact gatcaaaatg
    24241 aaatcctccg ggatttgcgg aagcgatgtc cactatatct atcatcaaca ccgtgccacg
    24301 gcggcggcac ccgataaacc gttataccag ggatttatca acggtcatga accgtgcggg
    24361 cagattgtgg cgatggggca aggctgccgc cattttaaag agggcgaccg cgtgctggtg
    24421 tatcacattt ctggctgtgg tttttgcccg aactgccgcc gcggctttcc tatctcttgt
    24481 actggcaaag gaaaagcggc ttacggctgg cagcgtgacg gcggtcatgc cgaatatctg
    24541 ctggcggaag aaaaagatct gatcctcctg ccggatgcgc tgagctacga agatggtgcg
    24601 tttatcagtt gcggcgttgg tacagcgtat gaaggaattt tgcgcggcga agtttccggc
    24661 agtgataacg tgctggtggt cggtctgggg ccagtcggca tgatggcgat gatgctggcg
    24721 aaaggtcgcg gtgcaaaacg gatcatcggc gttgatatgc tgccggaacg tctggcgatg
    24781 gcaaaacagt taggggtgat ggatcacggc tatttagcaa ccaccgaagg tctgccgcag
    24841 attatcgccg aactcaccca cggtggcgcg gatgttgcgc tcgattgttc tggtaatgcc
    24901 gcaggtcgct tgctggcact gcgatccacc gctgactggg gacgggtggt ttacatcgga
    24961 gaaaccggaa aagtggagtt cgaggtcagc gccgatctga tgcaccatca acggcggatt
    25021 atcggctcct gggtgaccag tctgttccat atggaaaaat gcgcccatga tctgacggac
    25081 tggaaactgt ggccgcgtaa cgccattacc catcgcttct cgctggaaca ggcaggtgat
    25141 gcctatgcgc tgatggcgag cggcaaatgc gggaaagttg tgattaactt cccggattaa
    25201 ggagttagcg gatgaccatc tataccttat cccacgggtc gctaaagctg gacgtttctg
    25261 accagggcgg cgtaatcgaa ggcttctggc gcgatacgac gccgctctta cgtcctggta
    25321 aaaaaagcgg tgtggcaacc gatgcctcat gttttccgct ggtgccattt gcaaaccggg
    25381 tgagcggcaa tcgttttgtc tggcaggggc gtgagtatca actgcaaccg aatgttgagt
    25441 gggatgcgca ctatctacac ggcgatggct ggctgggcga gtggcaatgt gtctcgcaca
    25501 gcgatgatag tttgtgtctg gtgtatgaac atcgcagcgg tgtctatcac tatcgggtaa
    25561 gtcaggcgtt tcatttaacg gcggatacgc tgacggtgac gctctctgtc accaatcaag
    25621 gggcagagac gctgccattt ggtaccggct ggcatcctta ttttccgttg tcgccgcaaa
    25681 cgcggattca ggcacaggcg agcggttact ggctggagcg ggagcagtgg ctggcgggtg
    25741 agttttgcga gcagctaccg caggaactgg attttaacca gcccgcgccg ttgccgcgcc
    25801 agtgggtgaa caatggtttt gccggatgga atggtcaggc ccgcatcgag cagccgcagg
    25861 aaggttatgc catcatcatg gaaacgacgc cacctgcacc gtgttatttc atctttgttt
    25921 ccgaccctgc gtttgataaa ggatatgcgt ttgatttctt ctgtctggaa ccgatgagcc
    25981 atgcgccgga tgatcatcat cgcccggaag ggggtgatct cattgcgcta gcaccagggg
    26041 aatcaacaac ttcagagatg tcgttgcggg tagagtggct gtaatttatt aaatgccgga
    26101 taaggcgttc acgccgcatc cggcatcttt cctaacgccg atcgagagaa attgcccccg
    26161 gcccggtaat tgccagcagc aggaatgcgc cagcgatact gacattcttc cagaaattaa
    26221 tcatatttgg cccaaccgca tcgccggtca tatcccagta atggtgacca atcaccgccg
    26281 tacccagcgt gtagaaaata aacagcaccg ccagcggacg ggtgaaaaag ccaagcacga
    26341 ttaatatcgc ggcgggcact tccataacta ccgcaataat cgctgccagc atcggcattg
    26401 gcgcgcccaa cgaggccata tattggaccg taccgtcaaa gcccatcatt ttgggaaaac
    26461 caaaaataat gaaaattaag accacggcga tacgggcaat taataacaaa acggggcggg
    26521 cagctccaaa atcaaaataa cgtagtgtgt tcataaccaa tccctttgca agataatgtg
    26581 atttaaacgt aatacacact cttgcacctc gccacgcccc aggcattgag atattcgcta
    26641 attatcctgg ttacagtgat ttaagcgcga tgttctcatc tatcagcagt ttcgcatcaa
    26701 acgttttgcc gctctggatc catttgcgaa ttgggcgaat ctcacgcccc tgattcagcg
    26761 tgaccgcacc gataagcacg ccgttttgca gattaaacca aatcgccttc tgagtttccg
    26821 ggttgccacg acaaagccag tcatcgccac gcatatcgcc aataaactgt aagttatcac
    26881 tgtactgatc gctccagaac cacggcggcg gcagtagcgg tagcggtagc cctaacattg
    26941 cggcagcggc aatttgcgcc tggttattgg cgttttccca gctttcgcag cggtgtagtg
    27001 caccattatc aagacgagtg attgccacat cgccaccggc aaagatcgcg ggatcgcagg
    27061 tgcggcaagc ctcatcaatg acaatgccat tggcagtatc aaggttggcc tcgcgagcca
    27121 gttgctcgtt ggcgctgata ccaataccgt aaatcaccac atcagcctga agcgtttccc
    27181 cactttgcag cgtcagttct actttttcac catcgaccac atgttcaatg gcattattga
    27241 gcagaatgcg cacaccagcc tgctggtggc gctgtaaaag atagcgttgc acgggcggtg
    27301 gtgcattacg gcccatgacg gttgccgcca gttcaatcac tgtcacctta catctgcgct
    27361 gcgtggcgct ggcagccagt tccagaccaa tagttccggc accgacaatc acgactgacc
    27421 gttcgggctg cagaacttct cgcagtctgg cggcatcacc ggcatggcgt agggtaaagc
    27481 agcgttctcc cagtgcatca agcaacggca gcggtcgagc tgccgcgccg gttgctataa
    27541 aaagctgatc ccagtgccag ctttcgccgt tggttaacac taactctcgt gtgtcgcgac
    27601 ccagcgtttt gatggttaca ccggaatgca gatgaacatt gttttcctgc caccagttag
    27661 cgggtaacac ctgctgtaac tgcggggaat cttccagcaa catggatttc gagagtggag
    27721 gtcgttcata aggaagatgt cgctcatcgg aaaacagatg cagctcaccg gtgaaccctt
    27781 gctggcgtag cgaggccgca gccattgccg ccgcttgccc gccaccgaca ataatgatgg
    27841 ttttttcttt catcgtgctt ccttaaagat ccagcccagc cgctacgtgg cgaatgccgc
    27901 gaatcgctaa acccgcatca gcgttgatca tcacaccgct taatgcgcga ttattgcgcc
    27961 gcgatgccaa catcacatac ggccccgtaa aatccgccgg ttgcgggaaa aattgcagcg
    28021 gtaaaatggc ggcaattttc tccggcgtca gagactgcat tatcgaggtt tcactttgcc
    28081 cgagcgcctg tgggccgcgc aggtcgctgg ccataccaca cgggccgacg ccattcaccc
    28141 gcactttcgg tgccagttca taagccagtt ggcgaataag tccggttgcg gcatgtttac
    28201 tggcggtgta cagcgggcca ccgccgccag gataccaggc ggcatttgac agtgtgaaaa
    28261 tcatgctgcc ttcactggcg attaacgccg gagcgcaggc ttttgcgccc agcaggtaac
    28321 cgagaacatt gacgttaaac agctcgtgga agccggtttc gagcgtctct gcgggagtat
    28381 taaccagtga ggcattgtga tcccagatgc ctgcattgcc gataaaacaa tccagcttgc
    28441 cggaacgagt caggatctga tcgaccgcgc gttgataatc ggcataacag gtcacgttac
    28501 cttccaccgc cagaatatgt tcgccaaatc gctgacgcag actggcgact tttgccgccg
    28561 acagttccag cgtggcaacc tgcgcgcctt cttcgataaa tcgctcgacc agcgccagcc
    28621 ctaatcccga tccgccgccg gtaataaaaa tggactcgtt atgcagatcg ctcatggctg
    28681 cgcctccggt aagtcgatga aaatgtcacc accttcaacg tgtactggat aagtggtgag
    28741 cggatcggtg gcgggcaggc ataacgcttt ccccgttttc aggcaaaaac tggcggcgtg
    28801 tagcgggcac tccaccgtgg cgtcatcttc cagataccct tctgacattg acgcattacc
    28861 atggctgcaa cgatcgttaa tggcataaaa ctcgccgcca acgttgaaca gggcgatgac
    28921 gggcgaggta tcgatccgga gagcctcacc ctccggcaca tccgcaacgg gacacgcata
    28981 aattcgattc atcagaacag tacactcagg ttatgggaag tgattaccgc ttgatccagg
    29041 acgatatccc gttccagcaa gcgccagttg tcatcttcca gacggcgaac tttgtcgaaa
    29101 cgcgttccaa catagaatgt ttcatcgcgc tctttttgtg cccgataaag cagataattt
    29161 acccgcacag caaatacgtt tgggatgtcg gtttcgctta tctggcagtt gctgattaag
    29221 tgacgggtgc gtgacggcgg ctcttctgcc caggccatgc ccgtttccag acgggcgatt
    29281 cgccgctcca gctggtcttt ggtgtcatta aaaatccagg ttgtcggtgg ctggacgcct
    29341 ttgcggcggt cgcgtgtttg cgcgttaact gtggtgcgca tggtgtaacg aatctcttcg
    29401 tcgagctgcg ccagccagtc acgaaatttc cagtcgtcca gtaagctggc ttcgtgaaag
    29461 agaaactggc taatgcggtg atgtaactct agtgaaactt gcgcactcat ttcatcacct
    29521 cctgctggta agcggcggtt ttatcgagca cttcctgcca gctttcgcta ctcagaagat
    29581 cagcccagcg ttggtacatt ccacgagcgg cagtttctga aaagatatag ttagtaatgc
    29641 caggaatgcc gtcgtcgcgg cgcttttcct gaccaagccc catttccaga cacagtttgc
    29701 tgttgcgggc gcggtggcct ttaagcaatt tctggatttc acaccagttc tccgagtcat
    29761 cctgctcgag aaaaccagca ggaccaaaag cacgagtggc gctgttttca aaagcggctt
    29821 taacttcatc ggaggcggct ttgtcagtaa tacagaacgc ccacacttca acttgatcag
    29881 ggccgcgcgg atgccagacg cggagtgtgg cagtgccgtt tagccatgaa agcgtgggga
    29941 atatattgtt atgacccgcc aggcgcaggg cgcgaacttc acctaaacgt tgttctgctt
    30001 cggcataggt ttcgcggtaa tagcttgaaa ctgcgccatc gacccagaca ttagcatccg
    30061 gtttttcagt aaagaagaaa ccgctaccgt gaccgtcctg accaaattgc agcgcatctt
    30121 tggcggtttc ccacaccggg cgggcggttt gtccatcacc gaggcgctta tcgctgccat
    30181 catctttcgc ccctaatacc tgaacggcag aagcatggct gaacagagca tgatactggt
    30241 cactggcgaa ctgctctgcc gggaatttcc agttacagtt gatcacccac ttttgtacgc
    30301 cgccgacaat ttcggtgccg ccttcgcgac gatccagcat gccatccaga taccaggcaa
    30361 tgtcacccag gtaatcacgc aggcccggtg cgctggtatc ccagttgcca aaaatcagcc
    30421 ctttataact ctccacacaa ggaacttcgt ttagtcccca gtgggattta cacaaccctt
    30481 gtgggtaggc gcgaggttcc agcggtacat cgatcaactc gccgttaatg ccataagacc
    30541 agccgtgata cggacaggta aaggcgcgag tgttgccgca atctgcataa ctcacacgca
    30601 tggcccggtg gcggcattgg ttgagaaacg ccttaatgct gccgtctttc tgacgcacta
    30661 cgacaaccgc atcttccccc atgtaggtgt taaagaaatc accaggtttt gggatctggc
    30721 tttcgtgggc gagaaataac cagcaacggc cgaaaatacg ttcaagctcc agttggtaaa
    30781 tatctgggtc ggtataaata cgcggagtga cccgaccatt ttgggtatca atcagttggt
    30841 aaatgttcaa atctgagggt gtggtcatat tttttaatcc tgaaagataa gatgtattga
    30901 ccttctattt ttgtgccata aggatgttgc tggttggttg ctaatatgtg aaatagttaa
    30961 tttctaccag ctgagacgtt ttttcgataa caaggggaag gtgatggaac tacggcattt
    31021 acgctatttc gtcgcagtgg cgcaggcact gaactttacc cgtgcggcgg aaaaactgca
    31081 tacctcacag ccttcgttaa gcagccagat ccgcgatctt gaaaactgtg tcggtgttcc
    31141 gttactggtg agggataagc gcaaagtcgc gttgacggcg gcgggagaat gttttctcca
    31201 ggacgcgctg gcaatcctcg aacaagcgga aaatgccaaa ttacgggcgc ggaaaattgt
    31261 tcaggaagac agacaattaa ccattggctt tgtgccatcg gcggaagtga atttactgcc
    31321 aaaagtatta ccgatgtttc gtctcagaca gccagacacc ttgattgagc tggtgagttt
    31381 aatcaccacg caacaggagg aaaaaattcg ccgtggtgaa ctcgacgtcg gcttaatgcg
    31441 ccatcctgtt tatagcccgg agattgatta tctggagctt tttgacgaac cattagtggt
    31501 tgtgttaccg gttgatcacc ctttagcaca tgaaaaagag atcaccgccg cgcaactgga
    31561 tggcgtgaat ttcgtcagta ccgatccggt gtattccggt tcgcttgcgc cgatcgttaa
    31621 agcgtggttt gcgcaagaaa acagccagcc aaatatcgtc caggtggcaa cgaatattct
    31681 ggtgaccatg aatctggtgg gcatggggct gggcgtcact ttgatacccg gttatatgaa
    31741 taattttaat accggacagg ttgtttttcg tcccattgcc ggaaatgtgc cttccattgc
    31801 tttactgatg gcgtggaaga aaggggagat gaagccggca ttacgcgatt tcatcgccat
    31861 tgtgcaggaa cgtttggcaa gcgtaacggc ataattacgc attatttttg caacatgcgt
    31921 cgcggatctc agttttttag tgcttgtgaa ctggcgttac agacatcggg aagcaggctc
    31981 gaatattctt atttttcaac ttgcaccaac attcgattaa ccccagacta acgcctcctg
    32041 acgggaggga ctcatggttt tgcaatccac gcgctggttg gcgctcggct atttcacata
    32101 cttttttagt tacggcattt ttctaccttt ctggagcgtc tggcttaaag ggattggttt
    32161 aacgccagaa accatcggcc tgttattggg ggcaggtctg gttgcccgtt tcctcgggag
    32221 tttgctcatc gcgccccgcg tcagcgatcc ttcccgcctg atttccgcct tgcgcgtgct
    32281 ggcactgctg acacttctct ttgctgtcgc cttctgggcg ggggcgcacg tagcgtggct
    32341 gatgctggtg atgattggct ttaacctctt tttctcacca ctggtaccgt tgaccgatgc
    32401 actggcgaat acgtggcaaa agcagttccc gcttgattac ggcaaagtgc gactgtgggg
    32461 ctcggtggcg tttgtcattg gctcggcgct gacgggcaaa ctggtcacta tgtttgatta
    32521 tcgggtgatc ctcgcgctgt tgacgttggg cgtggcatcc atgctgctcg gctttctcat
    32581 ccgtccgacg attcagccac aaggggcaag tcgccagcag gagagcaccg gttggtctgc
    32641 gtggttggcg ctggttcgcc agaactggcg ctttctggcc tgcgtttgtt tattgcaggg
    32701 ggcacatgcg gcctattacg gttttagcgc catttactgg caggcagctg gctactcggc
    32761 ctcggcggtg gggtatttgt ggtcgctggg cgtggtggcg gaagtcatta tctttgcgct
    32821 gagtaataaa cttttccgcc gttgtagtgc acgcgatatg ctgttgatct cggcgatttg
    32881 cggcgtagtg cgctggggca ttatgggagc aactacggcg ttgccgtggt tgatagtggt
    32941 gcaaattctg cattgcggca ccttcacggt ctgccacctg gccgccatgc gttatattgc
    33001 tgctcgccag ggtagcgaag tcatccgttt acaggcggtt tactctgccg tcgcgatggg
    33061 cggcagtatc gctatcatga ccgttttcgc cggtttcctg tatcaatatc tgggccacgg
    33121 cgtgttctgg gtaatggcgc tggtggcgct gccggcaatg tttttgcgcc cgaaagttgt
    33181 tccctcatgc tgattcgagc attttgcgga tctgctcctg ttgatgtgtt gtcagcgtca
    33241 ggtccgtata gatcagcggt ggtgaaaaga gcggtaatgg catggtataa ggcgcaataa
    33301 tcagtgccgc gccacgcgga gcgcctgtct gcaaaaatgc ctttaccgac atatgcttaa
    33361 tgttgagcgg cagtaacgtt agttcgcgta actgctgctc aatctgcgct tctcgctcgc
    33421 tatcattccc ggtcagtaga ataatctgtt tttcatgcag atcgttttcc tgcattagcc
    33481 aggcaccgaa aatcaccgcg accagaccac tttcctcatc agaaagatgg acgccgtatt
    33541 cactttcaaa tccggccagc gccgcgcggg tggtgcgcac caggcgtggg tacagtctgg
    33601 cgaactcttc cggcagggta ttatcaatac cgatggcaaa caaactgcga tttaacgcct
    33661 gggcgaggtg ggtataaagc tgatcacata acccctgttc atcataaaaa cgaacatttc
    33721 ccagctcacg aaaatggttt accagacgtt tgatagactg acgcaattgt ctgtctcgct
    33781 gatgcgcatc ccgcagtgga tcgggaaccc gcaacatgga aaaaagtagt gccataaaca
    33841 gtggctcatc aggcggtaca ggctggagcg cccgacgctg ccagtggcgt ccaatttcct
    33901 gcgctacctg aaattcaagg caggatttcg cccagcgacg ttggagcgga ttaaactgag
    33961 gcgtaattcc agcgtggtgc tgcagcaaac aatattgcag atacagacac aggaaatgaa
    34021 tatcgcgcga ttcaaagcgt ttttgcagcc gtcgggagca gagattcact aacgcttgta
    34081 gattggtatc gtcgtaaaag ttacgcgcaa ttccgcgccg cttcagttca ctcttcaggg
    34141 cgggggtgaa ttggctggta ataaatgacg gacacagacg gaaaccacgt cgtagccagt
    34201 gaaataaaca caaacgttgg ttaagcactg taccttcaac ccggtagcta ccgtcataac
    34261 cagtcgtcag tgtgagttga tgatagcgca ggatctcccg tcctgtttcg ctgatatcaa
    34321 gactggcaat atcatcatcc acaccattaa gctcgctgaa ggttgccatg gtggcggtca
    34381 accccggctg aaagagcgtc agcaagatct ggcagcggcg ctggggagcc gaaaggacag
    34441 atggtggagc aagcgtaggc atcatctgta gatctcccat aagcataatt gataagaata
    34501 gctaaagttt gctcagggaa atggcgaagg gaatgctaat catcagaaat gttggcaagc
    34561 gtcacagaat ttttctcatc ttaaactgtt ctgaccagtg gattagcatg tcttaaggtg
    34621 gttttgtgaa tgattatcgt gctagcgatc atgggttagt tctgcggatt caacgcgcta
    34681 aggataaagt ccaccatctg ttcgcgataa acatcgccgt ggcgatcgga aaaagcatca
    34741 atgtgttcgc catccgggat taggatcaac cgttttggct cttttgccag gctatacaac
    34801 ttttcgctgt gctgccatgg gataacgtga tcagctttac cgtgaatgag taaaagcggg
    34861 atcgggctga cgctggcgat ataattttcg ccgctgtaac tctcatcaag taagtagcca
    34921 ctgccgggga tcatctggtt ggcgatggtt gcataagagg caaatgtgga gtcgaggatc
    34981 accgcacgta tgccttcacg atcaccctga ccaataacag caagaatatt cgccccgcca
    35041 atactctgcc cgaacagcac cagccgttgt gggtttacat cactgcgatg gcgcaccaca
    35101 ttgatggcgc tttgcgtatc gtccagcaat ccggcctggg acggcgtgcc ttttgattta
    35161 ccaaacccgc gataatcaaa cataaaaacg ttgaaattac gctcgggtaa ccaactgacc
    35221 agcggccagt gggcggacat atttccggca ttgccgtgag catgaatgat ggttgcgatg
    35281 gcgttgtcag cagggcccgt cgaagaagga ataaaccagc cttgcagacg agtaccgtcc
    35341 ttagccgtaa attcgacgga ttccgccgac caggggtcgg gaccgtaaat tttatcgtca
    35401 ggataataga agaagttgat ggcaacgcgc ggcactaaat aaacgcagaa ggcaacaaca
    35461 aagagaataa acagaatttt gggaacgcgt ttgttcactg gcagtgccat aacctttcct
    35521 tgcgacttct ttatcatcca ttaatcgggg aaagatggtg ggagagtgac gaaagaaagt
    35581 aaagcggtga ggaagtattt cagaatagcg cctgaaaggc gagggcgggt gagtgacatc
    35641 acccgcctga gtcattaacg cttcagagcg tcgcttaact cgtcacgcat gttcgccagc
    35701 atggctttca caacacgtgg gttaccagca acgatgttac cggtcagcat atagttatga
    35761 ccaccggtga agtcgctgac gatgccgccc gcttcacgaa ccagcagctc gcctgccgcg
    35821 aaatcccacg gacgcagacc gatttcaaag aaaccgtcaa cacgacccgc agcgacgtaa
    35881 gccagatcca gcgccgcaga accggtacga cggaagtctg cacattcgtt gaacagtttg
    35941 ccgacgatgt tgatgtaggt agtggcgtac tgttttgctt tgaacgggaa gccggtcgcc
    36001 agaatggtac cgtcgagatc gcgagcggtg ctaccgcgca gacggtagcc gttcagctgt
    36061 gcgccctgac cgcgagtggc ggtgaacagt tcgttacgca taggatcgta taccacagca
    36121 acttcggtgc ggcctttgat acgaacggcg atagataccg cgaagtgcgg cagacgtttg
    36181 ataaagttgg tagtaccatc cagtggatcg ataacccatt gaacatcctg atcagtacct
    36241 tcaagttcac cgctttcttc ggtgatgatg gtgtgctgtg ggtaagattt acgaatcgtg
    36301 tcgataatca ccgcttcggc agctttatct acgttggtca cgaaatcgtt actgcctttc
    36361 tggctcgctt ctacagcgtc cggggtttca tagtttttgg caattaaatt acccgccttg
    36421 cgcgctgcgc gcacggcgat gttcagcatc ggatgcatcg gtctctctca ctggatgtta
    36481 aagaacggga aaacggcgca gagtatagca gcgagatcgg catctgtctt cgttttatga
    36541 taagatgcgt gaataatctt tagatgaaga aagacaatgc tgcaaaatat tcgaattgtg
    36601 ctggtggaga cgtcacacac cggcaatatg ggttctgttg cccgtgccat gaaaacaatg
    36661 ggattaacca atctgtggct ggttaatcca ctggtgaaac ccgactccca ggcgattgcc
    36721 ctggcagcag gggccagcga tgtgattggt aatgctcaca tcgtcgatac gctcgacgaa
    36781 gcgttagccg gttgtagcct ggtggtgggc accagcgcac gctcccgcac gctgccatgg
    36841 ccgatgctcg acccgcgcga atgcggcctg aaaagcgtcg ctgaagcggc aaataccccg
    36901 gtggcgctgg tgtttggtcg cgagcgcgtc ggcttgacca atgaagagtt gcagaaatgc
    36961 cattatcatg tcgcgattgc ggctaacccg gaatacagct cgctgaacct ggcgatggcg
    37021 gttcaggtta ttgcctatga agtgcgcatg gcctggctgg caactcagga aaacggcgag
    37081 caggtcgagc atgaagagac gccgtatccg ctggtcgatg atctggagcg tttttatggt
    37141 catctggaac aaacgctgct ggcaaccggt tttatccgtg aaaaccatcc ggggcaggtg
    37201 atgaataaat tgcgccgtct gtttagccgt gcgcgcccgg aaagccagga attgaatatc
    37261 ctgcgcggga ttctggcttc tattgagcag cagaataaag gtaataaggc ctgagggcta
    37321 agccagataa atgcacggac ggtcccctcg ccccttcggg gagagggtta gggtgagggg
    37381 aagaggccag cgctaatgcg aataattgcc ctcaacccgc ccatggtctg gaaggacaac
    37441 aggtacaaac gccacgataa aaaaatggca ctgaaggtta aatacccgac taaatcagtc
    37501 aagtaaatag ttgaccaatt tactcgggaa tgtcagactt gaccctgcta tgcaataccc
    37561 ccacttttac aataaaaaac cccgggcagg ggcgagtttg aggtgaagta agacatgaga
    37621 ctgacatcta aagggcgcta tgccgtgacc gcaatgcttg acgttgcgct caactctgaa
    37681 gcgggcccgg taccgttggc tgatatttcc gaacgtcagg gaatttccct ttcttatctg
    37741 gaacaactgt tttcccgtct gcgtaaaaat ggtctggttt ccagcgtacg tggaccaggc
    37801 ggtggttatc tgttaggcaa agatgccagc agcatcgccg ttggcgaagt gattagcgcc
    37861 gttgacgaat ctgtagatgc cacccgttgt cagggtaaag gcggctgcca gggcggcgat
    37921 aaatgcctga cccacgcgct gtggcgtgat ttgagcgacc gtctcaccgg ttttctcaac
    37981 aacattactt taggcgaact ggttaataac caggaagtgc tggatgtgtc tggtcgtcag
    38041 catactcacg acgcgccacg cacccgcata caagacgcga tcgacgttaa gttacgcgct
    38101 taatttaacc gtcctgtttc gtgttgcatc tgcgttgact gcacatactc actccggtca
    38161 cttacttatg taagctcccg gagattcgta agtttgtcaa cttgctgaaa cccgaaacag
    38221 ttaggttaaa gataacaaga tattagaatc aggccggggc gtatcgcgct ccgtaaacac
    38281 ggtcgtacat ccagccggta gcctggttcc ttgcattgag tgatgtacgg agtttataga
    38341 gcaatgaaat taccgattta tctcgactac tccgcaacca cgccggtgga cccgcgtgtt
    38401 gccgagaaaa tgatgcagtt tatgacgatg gacggaacct ttggtaaccc ggcctctcgt
    38461 tctcaccgtt tcggctggca ggctgaagaa gcggtagata tcgcccgtaa tcagattgcc
    38521 gatctggtcg gcgctgatcc gcgtgaaatc gtctttacct ctggtgcaac cgaatctgac
    38581 aacctggcga tcaaaggtgc agccaacttt tatcagaaaa aaggcaagca catcatcacc
    38641 agcaaaaccg aacacaaagc ggtactggat acctgccgtc agctggagcg cgaaggtttt
    38701 gaagtcacct acctggcacc gcagcgtaac ggcattatcg acctgaaaga acttgaagca
    38761 gcgatgcgtg acgacaccat ccttgtttcc atcatgcacg taaataacga aatcggcgtg
    38821 gtgcaggata tcgcggctat cggcgaaatg tgccgtgctc gtggcattat ctatcacgtt
    38881 gatgcaaccc agagcgtggg caaactgcct atcgacctga gccagttgaa agttgacctg
    38941 atgtctttct ccggtcacaa aatctatggc ccgaaaggta tcggtgcgct gtatgtacgt
    39001 cgtaaaccgc gcgtacgcat cgaagcgcaa atgcacggcg gcggtcacga gcgcggtatg
    39061 cgttccggca ctctgcctgt tcaccagatc gtcggaatgg gcgaggccta tcgcatcgca
    39121 aaagaagaga tggcgaccga gatggaacgt ctgcgcggcc tgcgtaaccg tctgtggaac
    39181 ggcatcaaag atatcgaaga agtttacctg aacggtgacc tggaacacgg tgcgccgaac
    39241 attctcaacg tcagcttcaa ctacgttgaa ggtgagtcgc tgattatggc gctgaaagac
    39301 ctcgcagttt cttcaggttc cgcctgtacg tcagcaagcc tcgaaccgtc ctacgtgctg
    39361 cgcgcgctgg ggctgaacga cgagctggca catagctcta tccgtttctc tttaggtcgt
    39421 tttactactg aagaagagat cgactacacc atcgagttag ttcgtaaatc catcggtcgt
    39481 ctgcgtgacc tttctccgct gtgggaaatg tacaagcagg gcgtggatct gaacagcatc
    39541 gaatgggctc atcattaatc ggtatcggaa tcaggagaat ttataatggc ttacagcgaa
    39601 aaagttatcg accattacga gaatccgcgt aacgtgggtt cctttgacaa caacgacgag
    39661 aacgtcggca gcggcatggt gggtgcaccg gcctgtggcg acgtgatgaa gttgcagatt
    39721 aaagtcaacg atgaaggtat cattgaagac gcgcgtttta aaacttacgg ctgcggttct
    39781 gctatcgctt ccagctccct ggtcaccgaa tgggtgaaag ggaagtctct cgacgaagcg
    39841 caggcgatca aaaataccga tattgctgaa gaacttgaac tgccgccggt gaaaattcac
    39901 tgttctattc tggcagaaga cgcgatcaaa gccgccattg cggactataa aagcaaacgt
    39961 gaagcaaaat aagagttgag gtttggttat gtcgattaca ctgagcgaca gtgcagcagc
    40021 gcgagtaaat acctttctgg ctaaccgcgg caaagggttt ggcctgcgtc tgggcgtgag
    40081 aacctccgga tgttcaggta tggcttatgt actggaattt gttgacgaac cgacgccgga
    40141 agacatcgtg tttgaagaca aaggcgtgaa agtcgtggtc gatggcaaaa gcctgcaatt
    40201 tctggacggt acgcagctgg acttcgtaaa agaaggcctg aatgaagggt ttaaattcac
    40261 caacccgaac gttaaagatg agtgtggttg cggcgaaagc ttccacgttt gatgcgcata
    40321 ccgataaccc caccgtggtc gcctgcgcgt ggggtttgtt ttacctgatt cgccgttatc
    40381 gcggcggatc gcagccctga gaatgttatg gattacttca ccctctttgg cttgcctgcc
    40441 cgttatcaac tcgataccca ggcgctgagc ctgcgttttc aggatctaca acgtcagtat
    40501 catcctgata aattcgccag cggaagccag gcggaacaac tcgccgccgt acagcaatct
    40561 gcaaccatta accaggcctg gcaaacgctg cgtcatccgt taatgcgcgc ggaatatttg
    40621 ctttctttac acggttttga tctcgccagc gagcagcata ctgtgcgcga caccgcgttc
    40681 ctgatggaac agttggagct gcgcgaagag ctggacgaga tcgaacaggc gaaagatgaa
    40741 gcgcggctgg aaagctttat caaacgtgtg aaaaagatgt ttgatacccg ccatcagttg
    40801 atggttgaac agttagacaa cgaggcgtgg gacgcggcgg cggataccgt gcgtaagctg
    40861 cgttttctcg ataaactgcg gagcagtgcc gaacaactcg aagaaaaact gctcgatttt
    40921 taatttctgg aagctaaaca tggccttatt acaaattagt gaacctggtt tgagtgctgc
    40981 gccgcatcag cgtcgtctgg cggctggtat tgacctgggc acaaccaact cgctggtggc
    41041 gacagtgcgc agcggtcagg ccgaaacgtt agccgaccat gaaggccgtc acctgctgcc
    41101 atctgttgtt cactatcaac agcaagggca ttcggtgggt tatgacgcgc gtactaacgc
    41161 agcgcttgac accgccaaca ccattagttc tgttaaacgc ctgatggggc gctcgctggc
    41221 tgatatccag caacgctatc cgcatctgcc ttatcaattc caggccagcg aaaacggcct
    41281 gccgatgatt gaaacggcgg cggggctgct gaacccggtg cgcgtttctg cggacatcct
    41341 caaagcactg gcggcgcggg caactgaagc cctggcaggc gagctggatg gtgtagttat
    41401 caccgttccg gcgtactttg acgatgccca gcgtcagggc accaaagacg cggcgcgtct
    41461 ggcgggcctt cacgtcctgc gcttacttaa cgaaccgacc gctgcggcta tcgcctacgg
    41521 gctggattcc ggtcaggaag gcgtgatcgc cgtttatgac ctcggcggcg ggacgtttga
    41581 tatttccatt ctgcgcttaa gtcgcggtgt gtttgaagtg ctggcgaccg gcggtgattc
    41641 cgcgctcggt ggtgatgatt tcgaccatct gctggcggat tacattcgcg agcaggcggg
    41701 cattcctgat cgtagcgata accgcgttca gcgtgaactg ctggatgccg ccattgcagc
    41761 caaaatcgcg ctgagcgatg cggactccgt gaccgttaac gttgcgggct ggcagggcga
    41821 aatcagccgt gaacaattca atgaactgat cgcgccactg gtaaaacgaa ccttactggc
    41881 ttgtcgtcgc gcgcttaaag acgcgggtgt agaagctgac gaagtgctgg aagtggtgat
    41941 ggtgggcggt tctactcgcg tgccgctggt gcgtgaacgg gtaggcgaat ttttcggtcg
    42001 tccaccgctg acttccatcg acccggataa agtcgtcgct attggcgcgg cgattcaggc
    42061 ggatattctg gtgggtaaca agccagacag cgaaatgctg ttgcttgatg tgatcccact
    42121 gtcgctgggc ctcgaaacga tgggcggcct ggtggagaaa gtgattccgc gtaataccac
    42181 tattccggtg gcccgcgctc aggatttcac cacctttaaa gatggtcaga cggcgatgtc
    42241 tatccatgta atgcagggtg agcgcgaact ggtgcaggac tgccgctcac tggcgcgttt
    42301 tgcgctgcgt ggtattccgg cgctaccggc tggcggtgcg catattcgcg tgacgttcca
    42361 ggtcgatgcc gacggtcttt tgagcgtgac ggcgatggag aaatccaccg gcgttgaggc
    42421 gtctattcag gtcaaaccgt cttacggtct gaccgatagc gaaatcgctt cgatgatcaa
    42481 agactcaatg agctatgccg agcaggacgt aaaagcccga atgctggcag aacaaaaagt
    42541 agaagcggcg cgtgtgctgg aaagtctgca cggcgcgctg gctgctgatg ccgcgctgtt
    42601 aagcgccgca gaacgtcagg tcattgacga tgctgccgct cacctgagtg aagtggcgca
    42661 gggcgatgac gttgacgcca tcgaacaagc gattaaaaac gtagacaaac aaacccagga
    42721 tttcgccgct cgccgcatgg accagtcggt tcgtcgtgcg ctgaaaggcc attccgtgga
    42781 cgaggtttaa tatgccaaag attgttattt tgcctcatca ggatctctgc cctgatggcg
    42841 ctgttctgga agctaatagc ggtgaaacca ttctcgacgc agctctgcgt aacggtatcg
    42901 agattgaaca cgcctgtgaa aaatcctgtg cttgcaccac ctgccactgc atcgttcgtg
    42961 aaggttttga ctcgctgccg gaaagctcag agcaggaaga cgacatgctg gacaaagcct
    43021 ggggactgga gccggaaagc cgtttaagct gccaggcgcg cgtcaccgac gaagatttag
    43081 tggtcgaaat cccgcgttac actatcaacc atgcgcgtga gcattaacag aggttagtat
    43141 gggacttaaa tggaccgata gccgcgaaat tggcgaagca ctgtacgatg cgtatcccga
    43201 tcttgatccg aaaacggttc gattcaccga tatgcatcag tggatttgcg atctggaaga
    43261 tttcgacgac gacccgcagg catccaacga gaaaatcctc gaagcgattt tgttagtctg
    43321 gctggacgag gccgaataat ttccgcgtca tgcttcacgc cgcagatgcg ttggctgcgc
    43381 tcgttcaccc cagtcacata gttatctatg ctcctggggc ttcactcact tgccgccttc
    43441 ctgcaacgcg aatcatttag cggaaaaatc ctggggctgc caactggcgg cccttttaca
    43501 aagaaggata actaaaatga cagaagcgat gaagattacc ctctctaccc aacctgccga
    43561 cgcgcgctgg ggagaaaaag caacttacag cattaataat gacggcatta ccctgcattt
    43621 gaacggggca gacgatctgg ggctgatcca gcgtgcggcg cgcaagattg acggtctggg
    43681 catcaagcat gttcagttaa gcggtgaagg ctgggatgcg gatcgctgct gggcattctg
    43741 gcaaggttac aaagccccga aaggcacgcg taaagtggag tggccggatc tggacgatgc
    43801 ccagcgccag gaactggata accgcctgat gatcatcgac tgggtgcgtg acaccatcaa
    43861 cgcaccggca gaagaattgg gaccatcgca actggcacag cgtgctgttg atctgatcag
    43921 caacgtcgcg ggcgatcgtg tgacttatcg gatcaccaaa ggcgaagatc tgcgtgagca
    43981 aggttatatg gggctgcaca ccgtcgggcg cggttcagaa cgttctccgg tattgctggc
    44041 gctggattac aacccaactg gcgataaaga agcaccggtg tacgcgtgcc tggtaggtaa
    44101 aggtatcact tttgactccg gcggctacag catcaaacag actgcgttta tggactcgat
    44161 gaagtcggac atgggcggcg cggcaacggt taccggggcg ctggcatttg ccattacgcg
    44221 cggactgaac aagcgcgtga agctgttcct ctgctgtgcg gataacctga ttagcggcaa
    44281 tgcgttcaag ctgggcgata tcatcaccta tcgcaacggt aaaaaagttg aagtgatgaa
    44341 cactgatgcc gaagggcgtc tggtgcttgc cgatggtctg attgatgcca gtgcgcagaa
    44401 accggaaatg atcattgatg cggcgaccct caccggggcg gcgaaaactg cgctgggtaa
    44461 tgattatcac gcgctgttca gttttgacga tgcgcttgcc ggtcgtttgc tggcgagtgc
    44521 ctcacaagag aacgaaccat tctggcgtct gccgctggcg gaattccacc gcagccagct
    44581 gccgtctaac tttgccgaac tgaacaatac cggaagcgcg gcgtatccgg caggcgcgag
    44641 cacggcggcg ggcttcctgt cgcactttgt tgagaactat cagcaaggct ggctgcatat
    44701 cgactgctcg gcgacttacc gtaaagcgcc ggttgaacag tggtctgcgg gcgctacggg
    44761 acttggtgtg cgcacgatag ctaatctgtt aacggcgtaa actaatatgc cggatgcggt
    44821 gtaaacgcct tatccggcct acaaattctg caaattcaac aaattgcgaa tcccttgtag
    44881 gcctgataag cgtagcgcat caggcatttg cgccccgaac gttgaatgaa ttgtggttca
    44941 tatgtccgaa acaaaaaacg aacttgaaga cctgctggaa aaagcagcaa ccgaaccggc
    45001 gcaccgcccg gcctttttcc gtactctact ggaatccacc gtctgggtgc ctggtacggc
    45061 ggcgcagggc gaggctgtgg ttgaagatag tgcgcttgat ttacagcact gggaaaaaga
    45121 agacggcacc agcgtcattc cttttttcac ctcgttagaa gcgcttcaac aggcggttga
    45181 agacgaacag gcatttgtcg taatgcccgt tcgcacattg tttgagatga cccttggcga
    45241 aacgctcttc cttaatgcca aactgccgac cggtaaagaa tttatgccgc gtgaaatcag
    45301 tttgttgatt ggtggagagg gaaatccgct gagcagccag gaaatcctgg aaggcggtga
    45361 atcgctgata ttatcggaag tcgcagagcc gccagcacaa atgattgatt cactcaccac
    45421 cttatttaaa accattaagc cggtgaagcg tgcttttatt tgttcaatta aagagaacga
    45481 agaggcacag cctaatttac ttattggcat tgaagccgat ggtgatatcg aagaaattat
    45541 tcaggcgacg ggaagtgtag cgaccgatac attacctggc gatgaaccaa tcgatatttg
    45601 tcaggtgaaa aaaggggaaa aaggaattag ccactttatt accgaacata ttgcgccatt
    45661 ctatgaacgt cgctggggtg gttttttgcg tgactttaaa cagaaccgga taatctaaaa
    45721 tatgccgccc taaagggcgg cattcgggct tacttgctgc tctgaaggtt gttaaccacc
    45781 agtaaattgt cgccggagag actccggtta ttatttactt gggaaaacag cgcaccagaa
    45841 acaataagta cgatacagaa gcaccgtatc ttcattgtgc ggtccatgcg aagaccgcat
    45901 cacgacagca ccactggcga tattgccgcg atagcaagcg ctgagacgcg cttgctctaa
    45961 cggtcgcgga acatcctttc cttaagccag cgcccgcttg cgagacggcg gaaagggccg
    46021 cagtggataa tacactaaca gaacaataat aactctcttt tgcttgacaa aaagagagtt
    46081 actggcgagt attgtctccg ctgagacgcg cttgcattac tacggtaata caacctgctg
    46141 gaaaggccgc gaaccagacc agcaataaaa aaccgccaaa ttcggcggtt ttttattgcc
    46201 tgtaaaatac ttatttcact ggctcaaccg gtaaatctgc ccgcgcgccc cattcactcc
    46261 atgcgccgtc gtacagtttc acgtttggca catccagcgt cgcgagtgct aacaaaacca
    46321 cggctgccgt tacaccagag ccgcagctga cgataattgg tttgtcgtag ctgacgccgc
    46381 gaccaaaaaa tatcgcatcc agttcatcgg tcgtttttag ttcgccttcg cgcaccagtt
    46441 ccgtccacgg aacattcagt gctccgggaa tatgtccgcg acgtaaacct gggcgaggtt
    46501 catcaacttc tgcgttaaaa cgtgcagccg ggcgggcatc aataatttgc gccgtatttt
    46561 catggcttgc caataataca tcggttactt tcaccacggc ttcaggatta aacgcggcgt
    46621 taaactctcc ttccggcagc tctactgcac cttcttctaa cagcagatca tcgcgctgcc
    46681 agcctgcaag tccacccccc agaatcgaca ctttctctac accaaaggtg cgcagcatcc
    46741 accatgctcg tggggctgag aaaagattac cttcgtcata gacaatcagg tgcttatcct
    46801 ggttaacgcc taattcacgc atcgccacgg cgaacgtttc cgggcgcggc agcatgtgcg
    46861 gaagcgggga agtgtgatca gaaagcgctt cgatatcaaa aaacactgcg ccgggaatat
    46921 gtccattcag atactcctga gcaacgttac gatcttcctg tccaggcgac gccatgcggg
    46981 catcaataat ctgaatttcc gggtcatcaa tatgttcggc gagccagtcg gctcctacaa
    47041 accatgtcgt ggacataggc atctccattt ttgccggggt taggctaaat tgtcggatgt
    47101 ttagtgaaaa gcgacaagcg aaagaggctg atttggctgt ctgagccaca attctcacta
    47161 aaattcggat tgtatgggcg tgatactgaa ttacataacg taaattctca cgcataataa
    47221 ttattcgacg ttgctaacgg gccattgtgg cagggatgaa aaatgaaaaa gttacgcgta
    47281 gccgcctgca tgctaatgct ggcgctggca gggtgcgaca acaacgataa cgcgccaaca
    47341 gcggtgaaaa aagatgcgcc ttctgaagtt actaaagcgg cctcttcaga aaacgcgagt
    47401 tcagcaaaac tctccgtgcc ggagagacaa aaactggccc aacagagtgc cggtaaggtg
    47461 ctgacattgc tggatctctc tgaagtccaa cttgatggtg cagccacgct ggtgctgacg
    47521 ttctccatcc ctctcgaccc ggatcaggat ttctcacgcg ttattcatgt cgtcgataaa
    47581 aaaagcggca aagtggatgg tgcctgggag ctgtcagata atcttaaaga gttacgttta
    47641 cgccacctcg aaccgaaacg tgatttgatc gttactattg gcaaggaggt caaagcactc
    47701 aacaacgcaa ccttcagtaa agattacgaa aaaactataa ctacccgcga catccaaccc
    47761 agcgtcggtt ttgccagccg tggttcgctg ctgcctggca aagtcgttga agggctgccg
    47821 gtaatggcgc tcaacgttaa taatgtcgat gttaacttct tccgcgttaa gccagaatct
    47881 ctgccagcat tcattagcca atgggaatac cgcaattcgc tggcgaactg gcagtcagac
    47941 aaactgctgc agatggcgga tctggtctac accggacggt ttgatctcaa tcctgcgcgt
    48001 aacacccgtg aaaaattatt gctgccgctg ggcgatatca aaccgcttca gcaggcgggc
    48061 gtgtatctgg ctgtgatgaa tcaggctgga cgttacgatt acagtaatcc cgcgacgctg
    48121 tttacgttaa gtgatatcgg cgtttcagct caccgttatc acaatcgtct ggatatcttt
    48181 acccaaagtc tggaaaacgg cgcggcccag caaggaattg aagtctcttt attaaatgag
    48241 aaagggcaga ctctgactca ggcaaccagt gacgctcagg ggcatgtgca gctggaaaat
    48301 gataaaaacg cggcattact gttggcgcgt aaagacggtc agacaacgct actcgattta
    48361 aaacttccgg cgctggactt agcagaattt aacattgctg gcgcgccagg ctatagcaaa
    48421 cagtttttca tgtttggccc acgcgatctt tatcgcccag gtgaaacggt aatcctcaat
    48481 ggtttgctgc gtgatgcaga cggtaaagcg ttgcccaatc aacccatcaa gttagacgtg
    48541 attaaacccg atgggcaggt actcaggagc gtcgttagtc agccggagaa tggcctctac
    48601 cactttacct ggccactcga tagcaatgcg gcaaccggta tgtggcatat tcgcgctaac
    48661 acgggcgata accagtatcg gatgtgggat ttccacgtcg aagattttat gccagagcgc
    48721 atggtgctga atctgaccgg tgagaaaacc ccgctaacgc cgaaagatga agtgaaattc
    48781 tccgtggtgg ggtactacct gtatggtgca cctgctaatg gtaatacttt gcaagggcaa
    48841 cttttcctgc gcccactgcg tgaagctgtg tcagccttac ctggttttga attcggcgat
    48901 atagctgccg aaaatctttc ccgcacgctg gatgaagttc agttgacgct ggatgataaa
    48961 gggcgcggcg aagtttctac agaaagccag tggaaggaaa cgcattcccc attacaggtt
    49021 attttccagg gtagtttgct ggaatcgggc ggtcgcccgg tgacgcgccg cgctgagcag
    49081 gctatctggc ctgccgatgc attgccgggg atccgtccgc agttcgcctc gaaatcggtt
    49141 tacgattatc gtactgacag cacggtgaaa cagcccattg ttgatgaagg cagtaacgcc
    49201 gcttttgaca tcgtttatag cgatgcgcaa ggcgtgaaaa aagccgtgtc gggcttgcag
    49261 gtgcgcctga ttcgcgaacg ccgcgattac tactggaact ggtcagaaga tgaaggctgg
    49321 cagtcacagt ttgatcaaaa agatctgatc gaaaatgaac aaactctgga tctgaaagcg
    49381 gacgaaaccg gcaaggtcag ttttccggta gagtggggcg cttatcgtct ggaagtcaaa
    49441 gcgccgaatg aagcggtcag tagtgttcgt ttctgggctg gctatagctg gcaggacaac
    49501 agcgacggta gcggcgcagt gcgacccgac cgtgtcacgc tgaaactgga taaagccagt
    49561 tatcgccctg gcgacaccat taagttgcat atcgccgcgc caacggcggg taaaggttat
    49621 gcgatggtcg agtccagtga agggccgctg tggtggcaag agattgatgt tcgggctcaa
    49681 gggctggatc tgacgattcc ggtcgataaa acctggaatc gtcatgatct gtatttaagt
    49741 acgctggtgg tacgtcctgg cgataaatct cgctccgcga cgccaaaacg cgcggttggt
    49801 gtgttgcatc tgccgcttgg cgatgaaaac cgtcgcctcg atctggcgct ggaaacacca
    49861 gcaaaaatgc gtcccaatca accattaacc gtgaaaatta aagccagcac taaaaatggc
    49921 gagaagccta aacaggtgaa tgtgctggtg tctgccgttg atagtggtgt gctgaatatt
    49981 actgactacg tcacgccaga tccgtggcag gcgttctttg gtcagaaacg ctatggcgca
    50041 gacatttacg atatttacgg tcaggttatt gaaggtcagg ggcgtctggc agctctgcgt
    50101 ttcggtggcg atggtgatga gctgaaacgt ggtggtaaac cgccggtcaa tcacgtcaat
    50161 attgtcgcgc agcaggcgct gccggtaacg ctcaacgaac agggcgaagg ctcggttaca
    50221 ctgccgattg gcgattttaa cggtgaattg cgcgtcatgg cgcaagcctg gacggcagat
    50281 gatttcggta gcaacgaaag caaagtgata gttgccgcac cggtgattgc tgaactgaac
    50341 atgccgcgct ttatggcgag tggcgatacc tcgcgtctga cgctggatat cactaatctt
    50401 accgataaac cgcaaaaact gaacgttgcc ctgaccgcca gtggtttgct tgaactggtc
    50461 agcgattcac ccgcagccgt tgaattagcg ccaggtgtgc gtactacgct gtttatcccg
    50521 gtgcgagcat tgccgggtta tggcgatgga gaaattcagg ccaccattag cgggttagcg
    50581 ttaccgggtg aaaccgttgc cgatcagcat aagcagtgga aaatcggcgt ccgtccggcg
    50641 ttcccggcac aaacggttaa ttacggtacg gcgttacagc ctggtgagac atgggcgatt
    50701 ccggcggatg gattgcaaaa cttctcgcct gttacgctgg aagggcaatt gttgttgagc
    50761 ggcaaaccac cgctgaacat tgcacgttat atcaaagagt taaaagcgta tccgtacggc
    50821 tgtcttgagc aaaccgccag cggcctgttc ccgtcacttt ataccaacgc agcccaactg
    50881 caggcgttgg gcatcaaagg cgacagtgat gagaaacgcc gtgcatcggt cgatatcggc
    50941 atttcccgtt tgctgcaaat gcaacgtgat aacggcggct ttgcgctgtg ggataaaaac
    51001 ggtgacgaag agtactggct gacggcttac gtgatggatt tcctggtccg cgcaggcgaa
    51061 cagggttaca gcgtgccgac agacgccatt aaccggggta atgagcgtct gctgcgctat
    51121 ttacaagatc cgggcatgat gtcgatcccg tacgcggata atctcaaagc cagtaaattc
    51181 gccgttcagt cttacgctgc gctggtgctg gcccgtcagc aaaaagcacc actgggtgcg
    51241 ctgcgtgaaa tctgggagca tcgtgcagat gccgcttctg gtttaccgct gctgcaactt
    51301 ggcgttgcgc tgaaaaccat gggtgatgca acgcgtggtg aagaagcgat tgtgctggcg
    51361 ctgaaaacgc cgagaaatag tgatgagcgg atatggctgg gtgattacgg tagtccactg
    51421 cgcgacaacg cgttaatgct ctccttgctg gaagaaaata aactgctacc cgatgagcag
    51481 aacactttgc tgaacacgct ttcgcagcag gcatttggtg aacgctggct atcgacgcag
    51541 gaaagtaacg cgttgttcct ggctgcccgt acgattcagg atttaccggg taaatggcag
    51601 gcgcaaacct ctttctcagc tgagccgctg acaggcgaga aaacgctaaa cagcaatctg
    51661 aatagcgatc aacttgccac cttgcaggtg agaaacagtg gcgatcagcc gttatggctg
    51721 cgtgtggatg ccagcggtta tccgcaatcc gcacctttac cggcgaacga tgtgctgcaa
    51781 atcgagcgtc atattcttgg tactgatggt aagagcaaat cgctggactc gttacgtagc
    51841 ggcgatctgg tgctggtgtg gttgcaggta aaagccagta acagcgtgcc ggatgcgttg
    51901 gtcgtggatc tgctgcctgc gggtctggaa ctggaaaacc agaatctggc gaacggtagc
    51961 gccagcctgg agcaaagtgg tggcgaagtg cagaacttac tgaaccagat gcagcaggcg
    52021 agtattaagc acattgagtt ccgtgacgat cgctttgtgg cggcggttgc cgttgatgaa
    52081 taccaaccgg taacgctggt gtatctggcg cgggcggtga cgccgggaac gtatcaggta
    52141 ccgcaaccga tggtggaatc aatgtatgtt ccccaatggc gggcgaccgg cgcggctgaa
    52201 gatctgctga ttgtccgacc gtaaatgcct cgcttgttaa ccaaacgcgg ctgctggata
    52261 acgctggcag ctgcgccatt tctccttttt cttgcagcgt ggggagcaga taaactctgg
    52321 cctctaccgc tgcatgaagt caatcccgca cgagtggtcg tggcgcagga tggtacgccg
    52381 ctatggcgct tcgccgatgc tgacggcatc tggcgttatc cggtaacaat cgaagatgtt
    52441 tctccacgtt accttgaagc gctgatcaat tatgaagatc gctggttctg gaagcatccg
    52501 ggggtgaatc cattctcggt ggcgcgcgca gcatggcaag atctcacttc gggacgggtt
    52561 atttccggtg gcagcacgct cactatgcag gttgctcgtc tgcttgatcc tcaccccaaa
    52621 acatttggcg gcaaaattcg ccagctctgg cgcgcgttgc aactggaatg gcatctgtct
    52681 aagcgtgaaa ttctgacctt gtatcttaac cgcgctccgt ttggcggtac gttgcagggg
    52741 atcggtgcgg caagttgggc ttatctcgga aagtcgcctg cgaatttaag ctattccgag
    52801 gcggcaatgc tggcggtttt gccgcaagcg cccagccgtc ttcgcccgga tcgttggccg
    52861 gagcgtgccg aagccgcgcg taataaagtg ctcgaacgga tggccgtgca aggtgcgtgg
    52921 tcccgtgagc aggtaaaaga gtcaagggaa gaacccatct ggctggcacc ccgacaaatg
    52981 ccgcaactgg caccgctgtt ttcgcgcatg atgctcggta aaagcaaaag cgacaaaatc
    53041 gttactacgc tggatgccgg tcttcaacga cgtctggaag aactggcgca aaactggaaa
    53101 gggcggttgc caccgcgcag ctcactggcg atgatcgtgg ttgatcatac cgatatgcgt
    53161 gttcgcggct gggtgggatc ggttgatctc aacgatgatt cacgctttgg tcatgttgat
    53221 atggtcaatg cgatccgatc gccaggatcg gtgctcaaac cgtttgttta tggtctggcg
    53281 ctggatgaag gcttgatcca cccggcatca ctgctgcaag acgtcccccg gcgcaccggt
    53341 gattatcgac caggtaactt tgatagcggt tttcatggcc cgatcagcat gagcgaggcg
    53401 ctggtgcgct cgctgaactt acctgctgtg caggtgctgg aagcctatgg accgaaacgg
    53461 tttgcggcaa agttacgcaa tgttggattg ccgttatatt tgcccaacgg tgctgcgccg
    53521 aatctttcac tcattctcgg cggcgctggt gcaaaactgg aagatatggc ggcagcgtat
    53581 accgcgtttg ctcgccacgg caaggcaggc aaattgcgct tacagcctga cgatccgctg
    53641 cttgaacggc ctttaatgtc gtcgggggcg gcgtggatca ttcgccggat tatggctgat
    53701 gaagcgaaac ccttgccgga tagtgccttg ccgcgcgtcg ccccactggc atggaaaacg
    53761 ggcaccagct atggctatcg tgacgcctgg gcgattgggg ttaacgctcg ctatgtcatt
    53821 gggatctgga ctggcagacc ggacggcacg cccgttgttg gtcagtttgg ctttgccagt
    53881 gccgtaccat tgttgaatca ggtcaataat atcttactgt cgcgcagtgc gaatctgccg
    53941 gaagacccgc gcccgaactc agtcactcgt ggcgttatct gctggccggg cggacagtct
    54001 ttgccggaag gtgacggtaa ctgtcgccgc cgcctggcaa cctggctgct ggacgggagt
    54061 cagccgccaa ctctattact gccggagcag gaaggcatta atggcattcg tttccccatc
    54121 tggctggatg aaaatggcaa acgtgttgcc gctgattgcc cgcaagcgcg acaagaaatg
    54181 ataaatgtct ggccgctacc gctggaaccc tggctgcccg catcagaacg tcgcgctgtg
    54241 cgcttaccac cggcctcgac aatttgcccg tcatacggtc acgatgccca gctccctctg
    54301 cagctgacag gcgttcgcga tggcgcgatt attaaacgtt taccgggcgc agcggaagcg
    54361 actttgccgt tgcaatcaag tggaggggca ggtgaacgct ggtggtttct gaatggcgaa
    54421 ccgttaactg aacgcgggcg caacgtgacc ctgcatttga cggataaagg tgattatcaa
    54481 ttgctggtga tggatgatgt ggggcaaatc gcgacagtga aatttgtcat gcaatagtct
    54541 gttttgtccc tgattgttgc taaaactcat tttattttaa aaaaatgtta ccttcatcaa
    54601 tagtcaacgg ccctgttgct cattataatc cgcgccatct cgtacgctgg tacagacaac
    54661 aacagaacaa tttacagagg taaaaatggc tattgaacgt actttttcca tcatcaaacc
    54721 gaacgcggta gcaaaaaacg tcattggtaa tatctttgcg cgctttgaag ctgcagggtt
    54781 caaaattgtc ggcaccaaaa tgctgcacct gaccgttgaa caggcacgtg gcttttatgc
    54841 tgaacacgat ggaaaaccgt tctttgatgg tctggttgaa ttcatgacct ctggcccgat
    54901 cgtggtttcc gtgctggaag gtgaaaacgc cgttcagcgt caccgcgatc tgctgggcgc
    54961 gaccaatccg gcaaacgcac tggctggtac tctgcgcgct gattacgctg acagcctgac
    55021 cgaaaacggt acccacggtt ctgattccgt cgaatctgcc gctcgcgaaa tcgcttattt
    55081 ctttggcgaa ggcgaagtgt gcccgcgcac ccgttaataa tttcgtaaat gccgcgtgca
    55141 aacgtggcat ccgtgcgcca gaatttgtac aatgcagcgc ccccggacga gcagccgctc
    55201 accggggcgt ttcttttttc aatcctccag gggccataac gtgtaataac gaggccggaa
    55261 tagcatatgt ctgaacaatt agtcacacct gaaaacgtca ccacgaaaga tggaaaaatc
    55321 aacctgctgg atctcaaccg tcagcagatg cgggagtttt ttaaagattt aggtgaaaaa
    55381 cccttccgcg ccgatcaggt gatgaagtgg atgtatcact attgctgcga caactttgat
    55441 gagatgaccg acatcaacaa agtgttgcgc gacaaactga aagaggtggc ggaaatccgc
    55501 gcaccggaag tggttgaaga acagcgttca tccgacggca ccattaaatg ggcgatcgcc
    55561 gttggcgatc agcgcgtcga aacggtgtat atcccggaag acgaccgtgc cacgctctgc
    55621 gtctcttcgc aggtggggtg tgcgctggag tgtaaattct gttccaccgc ccagcagggc
    55681 tttaaccgca acctgcgggt atcggaaatt attggccagg tgtggcgtgc ggcgaaaatc
    55741 gtcggcgcag caaaagtcac cggtcagcgc ccaatcacta acgtagtgat gatgggcatg
    55801 ggcgagccgc tgctcaacct gaacaacgtc gttccggcga tggaaatcat gcttgatgat
    55861 ttcggttttg gcctgtctaa acgtcgcgtc acgctttcta cttccggtgt agttccggcg
    55921 ctggataaac tgggcgatat gatcgacgtt gcactggcaa tttccctgca cgcgccgaac
    55981 gacgaaattc gtgacgaaat tgtgccgatc aacaaaaagt acaatatcga aacgttcctt
    56041 gctgcggtgc gccgttatct ggagaaatcc aacgccaatc agggccgtgt cactattgag
    56101 tatgtgatgc ttgaccacgt taacgacggc actgaacacg cgcaccaact ggcggaactg
    56161 ctgaaagata cgccgtgtaa gatcaacctg atcccgtgga accccttccc gggcgcgccg
    56221 tatggacgca gctcgaacag ccgtatcgac cgtttctcaa aagtgctgat gagctacggt
    56281 ttcaccacta ttgtgcgtaa aacccgtggt gatgatatcg atgctgcctg tggtcagttg
    56341 gcgggcgatg ttatcgaccg tacgaaacgt accctgcgta aacggatgca gggtgaagcc
    56401 atcgacatta aagcggtctg atagccatta cgccacggtt actttatgta ccgtggcgta
    56461 atcttttatg aatgaagtat taagttgcaa ttgtctggtc aatattaacg gtgcgttttt
    56521 gctgacttta aggcagtatg taacgatgca acagtaagtt agccttagtg aatgtgggct
    56581 ttgtcacgag cacacagacg gtcttatact gtatgataac ggttaactta acggatgttt
    56641 cgcggtgtgg gtgagcatga tggttcaccg gcatctcaat tctcatttaa acgtacctgc
    56701 agcgaatgaa tactgaagcc acgcacgacc aaaatgaagc acttactacc ggcgctcgcc
    56761 tgcataatgc tcgcgaacaa ctaggactta gtcagcaggc cgttgccgag cgactttgcc
    56821 tgaaggtttc cacggtacgc gacattgaag aagataaggc acccgccgat cttgcttcaa
    56881 cattcctgcg cggatatatc cgctcttatg cgcgtctggt acatattcca gaagaagaac
    56941 tgctgccagg gctggaaaag caggctccac tacgggctgc aaaagttgcg cctatgcaga
    57001 gtttttccct cggtaaacgc cgcaaaaaac gtgacggctg gctgatgacc ttcacctggc
    57061 tggtgttgtt tgtggttatc ggcctgagcg gtgcctggtg gtggcaagac cacaaagctc
    57121 agcaggaaga gatcaccact atggccgatc aatcttcggc ggaactgagc agtaatagcg
    57181 agcaggggca gagtgttccg ttaaatacgt cgacaactac agacccggct acaacgtcca
    57241 cgccgccagc gtctgtggat actaccgcaa ccaacacgca aacacctgcc gtaactgcgc
    57301 cagcaccagc tgttgatccg caacagaatg cggttgtttc gccttcgcag gcaaatgttg
    57361 ataccgccgc gaccccggca ccaacggcaa caacaacgcc agatggcgct gcgccgttgc
    57421 caaccgatca ggcaggcgtg actacgccgg cggctgatcc gaatgcgctg gtgatgaact
    57481 ttactgccga ttgctggctg gaggttactg atgctaccgg taaaaaattg tttagcggta
    57541 tgcagcgtaa agacggtaat ttgaacttaa ccggccaggc accatacaaa ctgaaaattg
    57601 gtgcgccagc cgcagtacag atccagtatc aagggaaacc tgtcgatctg agtcgtttta
    57661 tcagaactaa ccaggttgcg cgtctgaccc tcaatgccga acaatcaccg gcgcagtaac
    57721 agacgggtaa cgcgggagat ttttcatgca taaccaggct ccaattcaac gtagaaaatc
    57781 aacacgtatt tacgttggga atgtgccgat tggcgatggt gctcccatcg ccgtacagtc
    57841 catgaccaat acgcgtacga cagacgtcga agcaacggtc aatcaaatca aggcgctgga
    57901 acgcgttggc gctgatatcg tccgtgtctc cgtaccgacg atggacgcgg cagaagcgtt
    57961 caaactcatc aaacagcagg ttaacgtgcc gctggtggct gacatccact tcgactatcg
    58021 cattgcactg aaagtagcgg aatacggcgt cgattgtctg cgtattaacc ctggcaatat
    58081 cggtaatgaa gagcgtattc gcatggtggt tgactgtgcg cgcgataaaa acattccgat
    58141 ccgtattggc gttaacgccg gatcgctgga aaaagatctg caagaaaagt atggcgaacc
    58201 gacgccgcag gcgttgctgg aatccgccat gcgccatgtt gatcatctcg atcgcctgaa
    58261 cttcgatcag ttcaaagtca gcgtgaaagc gtctgacgtc ttcctcgctg ttgagtctta
    58321 tcgtttgctg gcaaaacaga tcgatcagcc gctgcatctg gggatcaccg aagccggtgg
    58381 tgcacgcagc ggggcagtaa aatccgccat tggtttaggt ctgctgctgt ctgaaggcat
    58441 cggcgacacg ctgcgcgtat cactggcggc cgatccggtc gaagagatca aagtcggttt
    58501 cgatattttg aaatcgctgc gtatccgttc gcgcgggatc aacttcatcg cctgcccgac
    58561 ctgttcgcgt caggaatttg atgttatcgg tacggttaac gcgctggagc aacgcctgga
    58621 agatatcatc actccgatgg acgtttcgat tatcggctgc gtggtgaatg gcccaggtga
    58681 ggcgctggtt tctacactcg gcgtcaccgg cggcaacaag aaaagcggcc tctatgaaga
    58741 tggcgtgcgc aaagaccgtc tggacaacaa cgatatgatc gaccagctgg aagcacgcat
    58801 tcgtgcgaaa gccagtcagc tggacgaagc gcgtcgaatt gacgttcagc aggttgaaaa
    58861 ataataacgt gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atagctcccg
    58921 aaacattgag ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca
    58981 aaaaacattc aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg
    59041 cagcgcattg aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc
    59101 ttgccgattg tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg
    59161 gttgaaaaag agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct
    59221 gaagggacgg cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa
    59281 cagcgtctgt ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat
    59341 cgtcagttcc atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct
    59401 gaactgatta tgctcaccgc ccgctggtgg cgcgcgctgg gtatttccga acacgtaact
    59461 cttgagctga attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg
    59521 gcattccttg agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact
    59581 aacccgctgc gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct
    59641 ccggcattag gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa
    59701 ctgcttgaga gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat
    59761 tactataacc gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg
    59821 tgtgcaggcg gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct
    59881 gtcggttttg cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa
    59941 tttaaagccg atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct
    60001 gcggctatgg cattagctga gcgtctgcgt gatgaattac cgggcgtgaa attgatgacc
    60061 aaccacggcg gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc
    60121 gttgctgtgg tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg
    60181 cgctctggtg agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta
    60241 ctgggttaag gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag
    60301 aagcggttaa acgctttttt gctgaaaatg gcaaagcact ggctgttggg gtgattttgg
    60361 gcgttggcgc actgattggc tggcgctact ggaacagcca tcaggttgat tctgcacgct
    60421 ccgcttctct tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca
    60481 tcccggcggc ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt
    60541 tggaacttgc gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac
    60601 aacaggggct ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg
    60661 ctcgcgttca ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca
    60721 aaggtgaagg gtgggctgcc attgttgccg acctgcgtgg tgaagcattg ctgagcaaag
    60781 gtgataagca aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg
    60841 cactgagcga aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg
    60901 caattgcgta aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg
    60961 ctgtttaaca gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag
    61021 tttacgccga ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc
    61081 aatcttcatc cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta
    61141 aaagcgctga atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat
    61201 ggctggttct ctaaagagcc tgcattactt tctggcggtc tgaccgtgtc tggtgggcat
    61261 gtctacattg gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg
    61321 gcatggcaaa ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg
    61381 gtgttaatcc acaccagtaa cggtcagtta caagcgctga acgaggctga cggcgctgtc
    61441 aaatggacag ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca
    61501 acggcttttg gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg
    61561 gaacagggcc agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt
    61621 gaccgtctga gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg
    61681 gcctataatg gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc
    61741 gaactgggtt cggtgaatga tttcatcgtc gacggcaatc gcatctatct ggtcgatcaa
    61801 aatgaccggg tgatggcgtt gaccattgat ggcggcgtta cgctgtggac acaaagcgat
    61861 ctgttgcatc gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtaggtgac
    61921 agtgaaggtt atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa
    61981 gttgatagtt ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag
    62041 gcaaaagacg gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa
    62101 acggctcctg gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt
    62161 tgtctgatga tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg
    62221 cgccctaacg taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg
    62281 gttgcggatt tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc
    62341 cgtgagttta tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc
    62401 cgcatggcgg aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg
    62461 gatgcgcgcg cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt
    62521 gaaaaaccga ccttcctggt ggcaaacaaa actgacggtc tggatcccga tcaggcagtg
    62581 gttgatttct actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt
    62641 ggcgtattaa gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa
    62701 gaggaagtcg acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa
    62761 gaagaagagg aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt
    62821 cgtccgaacg taggtaagtc tacactcact aaccgtattc ttggtgaaga gcgcgttgtt
    62881 gtttatgaca tgcctggcac gacgcgtgac agtatctaca ttccaatgga acgcgatgga
    62941 cgtgagtatg tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct
    63001 gtagagaaat tctccgtaat caaaacgttg caggccattg aagatgccaa cgtggtgatg
    63061 ttagtgattg atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt
    63121 ctcaatagtg ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa
    63181 gtgaaagagc aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt
    63241 gtgcacttta tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt
    63301 gaagcgtatg acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg
    63361 atggctgttg aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat
    63421 gcccacgctg gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac
    63481 ctgcctgatt cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg
    63541 ggatcgccga ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac
    63601 accctgacgc caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat
    63661 aaataattat ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggatggcta
    63721 ccggaggaat tcatatggaa ctgcattgtc cacaatgtca gcatgtgctt gatcaggata
    63781 acgaccatgc gcgttgccca tcctgcgggg aaattattga aatgaaagct ctttgtccag
    63841 actgccatca accgttgcag gtgttaaaag cctgcggtgc agtagattac ttctgtcagc
    63901 acgggcatgg cttgatatcg aaaaaacgcg ttgaattcgt gctggcttaa tgcacctttt
    63961 tacgcgattt ttttaccggc tggatgtttt ttacttcact ttctatccag ccgtcttcca
    64021 gacgagtggt tagcatttca cccgctttaa cttgcttcac ttttttcagt acattgccgt
    64081 cagtagcagt agtaacgctg tatccacgcg ccagcgttga cagtgggctt acggcttcga
    64141 ggtgcgttac tgcattaccg aaacgttcac gcgtggcgct aagctgtacg cgcagggttt
    64201 ctgctaaacg atattccagt tgctgaatgc gcgtttgcgc gcgatgaatc ttcggctgtg
    64261 gattttgctg attcagccgc tgtgttaacc gctgctgctg ttgcccggta cgcttaagtt
    64321 gattttccag cgcaaagctc atccgctttt gcaggcgctc aagcatggtt tgctggcgtg
    64381 ccagccggag ctgcggatgc tgttgctgta atcggtgatg gatctgcgta aagagacgcg
    64441 tgcggttggc gagataataa tccatcgcca tctccagccg ttgacgggtc gattgcacct
    64501 ggcgcagtaa ctcttgctga ttacggctca ctacttcagc ggcggcagac ggcgttggcg
    64561 cacgcagatc ggcaacaaaa tcggcaatgg tcacatccgt ctcatgcccg acggcgctga
    64621 cgaccggaat gcggctggca aaaatcgccc gcgctacgcg ttcgtcgtta aaactccata
    64681 aatcttccag cgaaccgccg ccgcgcccaa cgatcaacac gtcgcactca ttgcgctgat
    64741 tcgccagttc aatggcgcga acagtttgcc ccggcgcgtc atcgccctga acggcggtgg
    64801 ggtagatgat caccggtaga gaaggatcgc gacgttttaa cacatgcaaa atatcatgta
    64861 gcgcagcacc ggtttttgag gtgatcacac caacgcaatg cgcaggggag ggaagtggtt
    64921 ttttgtattg cagatcgaac aatccttcag cctgcaactt cgctttgagc tgttcgtact
    64981 tctgttgcag cagcccttca ccggccggct gcatactctc aacgattatc tggtagtcgc
    65041 cgcgcggctc gtagagcgta atattggcgc gaactaaaac ttgttgccca tgctgtggac
    65101 ggaaggtcac ccgacggttg ctgttgcgga acatcgcgca gcgtacctgg gcggtgtcgt
    65161 ctttgagtgt aaagtaccag tgaccggaag ccggttgtgt gaaattagag atttcgccgc
    65221 tgatccaaac ctgtcccatc tcatgctcaa gcagcagacg aaccgtttga ttcaggcgac
    65281 taacggtaaa aattgcaggg gattgagaag gtaacatgtg agcgagatca aattctaaat
    65341 cagcaggtta ttcagtcgat agtaacccgc ccttcgggga tagcaagcat tttttgcaaa
    65401 aaggggtaga tgcaatcggt tacgctctgt ataatgccgc ggcaatattt attaaccact
    65461 ctggtcgaga tattgcccat gctacgtatc gctaaagaag ctctgacgtt tgacgacgtt
    65521 ctcctcgttc ctgctcattc taccgttctg ccgaatactg ctgacctcag cacccagctg
    65581 acgaaaacta ttcgtctgaa tatccctatg ctttccgcag caatggatac cgtaacggaa
    65641 gcgcgcctgg ctattgctct ggctcaggaa ggcggtatcg gctttatcca caaaaacatg
    65701 tccattgaac gccaggcaga agaagttcgt cgtgtgaaaa aacacgaatc tggtgtggtg
    65761 actgatccgc agactgttct gccaaccacg acgctgcgcg aagtgaaaga actgaccgag
    65821 cgtaacggtt ttgcgggcta tccggtcgtt accgaagaaa acgaactggt gggtattatc
    65881 accggtcgtg acgtgcgttt tgttaccgac ctgaaccagc cggttagcgt ttacatgacg
    65941 acgaaagagc gtctggtcac cgtgcgtgaa ggtgaagccc gtgaagtggt gctggcaaaa
    66001 atgcacgaaa aacgcgttga aaaagcgctg gtggttgatg acgaattcca cctgatcggc
    66061 atgatcaccg tgaaagactt ccagaaagcg gaacgtaaac cgaacgcctg taaagacgag
    66121 caaggccgtc tgcgtgttgg tgctgcagtt ggcgcaggtg cgggtaacga agagcgtgtt
    66181 gacgcgctgg ttgccgctgg cgttgacgtt ctgctgatcg actcctccca cggtcactca
    66241 gaaggcgttt tgcagcgtat ccgtgaaacc cgtgctaaat atccggatct gcaaatcatc
    66301 ggcggcaacg tggcaacagc tgcaggtgca cgcgctttgg cagaagctgg ttgcagcgcg
    66361 gttaaagtcg gtatcggccc tggttctatc tgtacgactc gtatcgttac tggcgtaggt
    66421 gttccgcaga tcactgccgt tgctgacgca gttgaagccc tggaaggcac cggtattccg
    66481 gttatcgcag atggtggtat tcgcttctcc ggcgacatcg ccaaagctat cgcagctggc
    66541 gcaagcgcgg tgatggtagg ttccatgctg gcgggtactg aagaatctcc gggtgaaatc
    66601 gaactctacc agggccgttc ttacaaatct taccgtggta tgggttccct gggcgcgatg
    66661 tccaaaggtt cctctgaccg ttatttccag agcgataacg ctgccgacaa actggtgccg
    66721 gaaggtatcg aaggtcgcgt agcttataaa ggtcgcctga aagagatcat tcaccagcag
    66781 atgggcggcc tgcgctcctg tatgggtctg accggctgtg gtactatcga cgaactgcgt
    66841 actaaagcgg agtttgtacg tatcagcggt gcgggcattc aggaaagcca cgttcacgac
    66901 gtgaccatta ctaaagagtc cccgaactac cgtctgggct cctgattctc ttcgcccgac
    66961 ttcatgtcgg gcgatttata ttatctgttt cacttgcctc ggaataagcg tcaatgacgg
    67021 aaaacattca taagcatcgc atcctcattc tggacttcgg ttctcagtac actcaactgg
    67081 ttgcgcgccg cgtgcgtgag ctgggtgttt actgcgaact gtgggcgtgg gatgtgacag
    67141 aagcacaaat tcgtgacttc aatccaagcg gcattattct ttccggcggc ccggaaagca
    67201 ccaccgaaga aaacagcccg cgtgcgccgc agtatgtctt tgaagcaggc gtaccggtat
    67261 tcggcgtttg ctatggcatg cagaccatgg caatgcagtt gggcggtcac gttgaagcct
    67321 ctaacgaacg tgaatttggc tacgcgcagg ttgaagtcgt aaacgacagc gcactggttc
    67381 gcggtatcga agatgcgctg accgcagacg gtaaaccgct gctcgatgtc tggatgagcc
    67441 acggcgataa agttaccgct atcccgtccg acttcgtcac cgtagccagc accgaaagct
    67501 gcccgtttgc cattatggct aacgaagaaa aacgcttcta tggcgtacag ttccacccgg
    67561 aagtgactca cacccgccag ggtatgcgca tgctggagcg ttttgtgcgt gatatctgcc
    67621 agtgtgaagc cctgtggacg ccagcgaaaa ttatcgacga tgctgtagct cgcatccgcg
    67681 agcaggtagg cgacgataaa gtcatcctcg gcctctctgg tggtgtggat tcctccgtaa
    67741 ccgcaatgct gctgcaccgc gctatcggta aaaacctgac ttgcgtattc gtcgacaacg
    67801 gcctgctgcg tctcaacgaa gcagagcagg ttctggatat gtttggcgat cactttggtc
    67861 ttaacattgt tcacgtaccg gcagaagatc gcttcctgtc agcgctggct ggcgaaaacg
    67921 atccggaagc aaaacgtaaa atcatcgggc gcgttttcgt tgaagtattc gatgaagaag
    67981 cgctgaaact ggaagacgtg aagtggctgg cgcagggcac catctaccct gacgttatcg
    68041 aatctgcggc ttctgcaacc ggtaaagcac acgtcatcaa atctcaccac aacgtgggcg
    68101 gcctgccgaa agagatgaag atgggcctgg ttgaaccgct gaaagagctg ttcaaagacg
    68161 aagtgcgtaa gattggtctg gagctgggcc tgccgtacga catgctgtac cgtcacccgt
    68221 tcccgggacc aggccttggc gttcgtgtgc tgggtgaagt gaagaaagag tactgtgacc
    68281 tgctgcgccg tgctgacgcc atcttcattg aagaactgcg taaagcggac ctgtacgaca
    68341 aagtcagcca ggcgttcact gtgttcctgc cggtacgttc cgttggcgta atgggcgatg
    68401 gtcgtaagta tgactgggtt gtctctctgc gtgctgtcga aaccatcgac tttatgaccg
    68461 cacactgggc gcatctgccg tacgatttcc tcggtcgcgt ttccaaccgc attatcaatg
    68521 aagtgaacgg tatttcccgc gtggtgtatg acatcagcgg caagccgcca gctaccattg
    68581 agtgggaatg attttaatcc ctgcactatg aatgaacaaa accctctgtt actacagagg
    68641 gttttttatc ttcaagaatc ataggcttga agttactaac atcgattaat taaaccagct
    68701 gtccgatttg ttctcttctg ctttgcccac gcgtttcatc agatcgcgac cgccttcagt
    68761 catatttctg ttggcgtcag cttcagattg caccacatcg gtttgcgcag ctttgtgctt
    68821 cagttcctga tcgataaatt cgttttctcg cttaacgcgg gcttcttctt ttgccagcgc
    68881 cagtttttgt ttctgaatct ctaagctgcg tagctcatct tcataacttt gatcgcgttt
    68941 tttgtccgca gaggcttcgg cgtccagttt atcctgacga gctttcttat ttgccgctgc
    69001 cgttgccgct cttttgttag cggcggcctg ggcatttgcg cgacgttgct tctcttgctg
    69061 gatttccctg ttgcgctccg cgacccattc gtcatgctgc ctttgctctt catttttacc
    69121 ttgctgttca gcttctgcta cagcagagag ttgatcctgc aatgatgagg cgatagccgg
    69181 atagcttaag gaggccaaga tggcgcaaag aaaaactttc ttcatgactc ctcctgatta
    69241 ttagctcttt tcaggacatt tagtatttgg ctgaatacgc gtttcgttat acgttgtggt
    69301 aataacaacg gctaaacctg tcgtaaactg gcactcttta cccacctggg tagaggtata
    69361 cactttggtg ccttccttat atgttaaaga aacaccttcc actaaggttt tatcattcac
    69421 catagaaccc actgccgcgc ctacagctcc gccgccaact gcccctgccg tcgttccgga
    69481 attgctgcca gaaccgacgt tgtggccgat aacaccgcca gcgactgcgc caataagcgc
    69541 gccgaaggct tgtgcgttcc gtttattttg ggagttgtct acggcaactt ttgcgggaag
    69601 aatggaaata atattaacgg ttttagtttc ttgtttggta ttcagttgat cggtttgata
    69661 aacatcggcg gcatgatcgt cagcatttga ctggcatcct gccagagtga atgacgctaa
    69721 cattgccaca ggcagaagac attttttaaa tttcatcact attccttgtt atacacatta
    69781 cgaaaaataa gacatttaag atcatcaaaa gaggaaagta aatgtattgc gtataaaggg
    69841 atagattaaa tttattaaaa ttcacgcagg gaataattta taaaaatata tcattacaac
    69901 atatatatta attttatatt tatttcgcag ccaaacagga taaacatcag tcaggacaaa
    69961 gacgtaaaat ttaagatgaa agaatatctg ccgctttgat aattccaaag agcaaaagca
    70021 tccgacagcg aagtgccgtt aggttctgtg aaagtggaga aggtaaccgc taacgttgta
    70081 ccggtgattc gacggagagt gtggattgct gagcttcttt tttgctctga tggtgctgcc
    70141 aggcaccgac ggacgagtag ataaaacggc caaagaagaa gataaagctg atgagcagta
    70201 cgatacgggt catgcgactg ttaaatcggt gtcgttttcg catactggtt gcctgactca
    70261 caaaaggttc cttgaagtat gccccacgct gtggacggta cttacattaa ggcacaacag
    70321 gacaaaatgg tcaattcttc gttatgtaaa aaagcgtcag tggatacata ttttaatgtt
    70381 atggaagtta atttaattat ttacaatgat gatgtaataa tgatgaatta acatgataaa
    70441 cgtaataatt cattaatcag ggttattttg tttgatatat atcaattgga actttcatat
    70501 gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag tcaggtggga
    70561 tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat ctatgaaact
    70621 gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt tcctgccttc
    70681 tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt ccggtgaggt
    70741 ttttctcttt tatctgcctt tggcactgat gatcagcatg atgatgtttt tcagctgggc
    70801 ggcattgcca gggatcacct tagggatttt tgtccgcaaa tatgcagagc tgggttttta
    70861 cgaaacgctc tcattaacgg ctaattttat tatcattatc attctctgtt ggggcggtta
    70921 cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt taatttccca
    70981 gcgtatattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt tccagtttgc
    71041 tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc cttttaacct
    71101 cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg tcccgctgtg
    71161 ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt attcgcaatt
    71221 aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc tactggcatt
    71281 aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac
    71341 caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcgatgc gctatggtta
    71401 taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca gctatcaaaa
    71461 ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct ccagttatct
    71521 ggtattctct tttattgtca attatatggc tgtactggca actcgtcagc gagcggtagt
    71581 cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa atgttcgcgc
    71641 cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac gcatccctgg
    71701 catggaaatg ctggttaaga actatggcat catgctgcgg attcaataca agcaaaaact
    71761 ttctcactgg ctgtcaccct tgctggaacc gggtgaagat gtttatcagc tttcgggtaa
    71821 cgatctcgcg ctgcggctga atacagaatc gcaccaggag cgcattaccg cactggatag
    71881 ccatctcaag caatttcgtt tcttttggga tggcatgccg atgcaaccgc agattggcgt
    71941 cagttactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg gagagctaaa
    72001 tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa aatatgcagc gtcgcggggc
    72061 aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcgac tacagcaggc
    72121 gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc gtggtgatgt
    72181 ttaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactgatcg gccccgatag
    72241 cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt gggtcattga
    72301 gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc gttttgctat
    72361 taatctgtct ccaacctcgg tatgtcaggc tcgttttcct gttgaagtca gtcagttgct
    72421 ggataaatat cagattgaag cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct
    72481 gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag gctgccagat
    72541 tgcgattgat gatttcggca ccggctacgc cagctatgcg cggcttaaaa atgtgaatgc
    72601 cgatctgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata gtctggatta
    72661 tcagatagtg gcatcgattt gccacctggc gcgaatgaag aaaatgcggg tagtggcaga
    72721 gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg attatatgca
    72781 gggttatctt attggtaagc cgcaaccgtt aattgatacg ctgaatgaaa tcgaacccat
    72841 tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa agcattaagc
    72901 ggcgatttca ggtgtacttt cttcttcaat tttcaaccgc cagccagcca cgccttccca
    72961 gtattcttgc tccttttcca gatcaagcag taccagcgca ttctgactaa accagtcatg
    73021 cgggaaacgc agtgtccagt gactgtcatc agtaatcagt gtcaatgttg gcggtgtggt
    73081 ggttgcctga cgttgattgt ttagtaatac gccaaggcgc aatagctgta tcagtggcag
    73141 gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagcttaa tcgctttacg
    73201 gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc tgattaaaac ccggcaagtc
    73261 actgttttgc agaatataag cggagtggcg atgcaaaccg ctgtggttga tattcaaccc
    73321 gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat gcgccagctt
    73381 cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca ctcgtcgggc
    73441 ctgttcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac tacgcacatc
    73501 ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg ccccgtcaga
    73561 aaggcgcagt tcacggatgg ctaaagcatc aaacacacca cataaaatcg ccagtccagg
    73621 aacgaagact gttttccgct cttcggaaag acccggtaaa ctcagcgatg cgaaattacg
    73681 gtgacgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta tcccgtcttt
    73741 ctcgcccatt tccattaata cttcatgggc ggcttttatg gtaccggaag cgcccattgc
    73801 cacgttccag ccctgaatac ggaattgcca ggttaaagtt tccagttttt gtgctgccgc
    73861 catgcgagcg cgctgaaaat tctctttatt gatgaccccg ccagggaaat aaagctgggc
    73921 aaagctgaca caacccatcc ggcggctttc aacgagaata ggttcgaaat tttcaccaat
    73981 caccagttcc gtagatccgc cgccaatatc aataaccagt ttgcgacctt tttccggttg
    74041 ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa taatttcaat
    74101 cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca gcgcctgacg
    74161 cagtgtatgg gtgccaacta tacagacgct ggcaggagaa aacccttgta gccgttcggc
    74221 aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca tattatctgg
    74281 ccccaggccg tccgccagat gcacccgctg tttcaggcgg ccaataatct gcatggcacc
    74341 atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga ccgcagcaaa
    74401 ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt tgttcgagtg
    74461 atttgatgta gtcataaatc gccaactgcg cccgtacttt gcggcgattg ccgcggggaa
    74521 cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg ctgaacaata
    74581 tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggt gtcgccactt
    74641 caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag acctttttat
    74701 cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca acaatactga
    74761 tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac attccgcgaa
    74821 ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca accaggcctt
    74881 tatcgacaag gttatttagc ttcagggtga taccactggg cagcccttgc tgcacgttgg
    74941 cgatttcgcg gtctaccatt tcatataaca gacggcgaga gttttgcggt gacaccatta
    75001 aataatcaaa tgtcactggg cggtatgggt tttcaataaa gttaaatacc cgccgtactt
    75061 cgttggtgat acgcgcatct gcggtcagca acgaatagtc agtataaaga cgcgcggttt
    75121 tttcgttaaa gttcccggtc ccgatgtgtg cgtaacggac cacttcaccg ttttctttac
    75181 gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg cgcagagaag ataacgtgca
    75241 cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc ttcttcgtcg aaacgcgcct
    75301 gtaactcaac caccacagtg actttcttac cgttatgtgc ggcgtggatc atcgagtcga
    75361 tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt aatcgccagt acgctcgggt
    75421 cgaacgaagc ctgacgcagc agttccagca catgctcaaa ggtgtgataa ggataataga
    75481 gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct ttatcaaacc
    75541 aaatgtggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg acattgggga
    75601 aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag tcgtagcggg
    75661 aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg cgctgataaa
    75721 caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc aactccatca
    75781 ggctggcttc catctcatgc actaaatcgt attcggcatc gcgtgtcatc ttcattgaat
    75841 aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcaagg cagtaacgca
    75901 gaatgttatc cagaagaatc atcggcttgc gtcgacgcgg cgcttctggc ggtaaattca
    75961 caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg gtatcaccac
    76021 ggataatttc caccgccaga taggtgtaat catccttcag gaactgcact aagtcagtgt
    76081 caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa taatgacgaa
    76141 gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc tggttgcgcg
    76201 ccatctccag caatagctcg ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc
    76261 gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt tcttcgctaa
    76321 taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca aggttattgg
    76381 aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc gccgcttcct
    76441 gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat agcttttcct
    76501 gacccattac cacttttact ccgttttatt cacgggacac tgcctggata ttatggcgag
    76561 caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct ggagcttgcg
    76621 atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta cggggcatta
    76681 ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg cggcgttttc
    76741 gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa tagcgtgttc
    76801 ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca ccagcaaata ccgggacttt
    76861 cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca ccgatgtacc
    76921 gtgctcttca tcaccatttt ccagcgcttg acgatgggtg tgtaatccgg gatatttcgg
    76981 cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa acgccgggct
    77041 gagaatgcgc ataaaaccag ccagcacgac cacatcgggt gcgtacatgt cgatttcatg
    77101 aatcaactcc cggtcatagg cttcacgact gtcaaacgcg ctggcgatga gcgtatgcgt
    77161 tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac
    77221 tgcccgtacg gtgcctttaa ttttgttggt tttacaggcg tcaataattg cctgtaaatt
    77281 acttccgtta ccggaaataa gcaccacaat attcattatt cgataaccac gcgttgttcg
    77341 gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttggc attgagcagg
    77401 gcgagggctt tgtccacttc cggagcaggc agggcaataa tcatcccgac gccgcagttg
    77461 aaggtgcgat acatttcatg gcgctcaacg ttacctgccg tttgcagcca gttgaacact
    77521 tccggccact gccaggaaga ttcatcaatc actgcctgag tattatctgg caatacgcgt
    77581 ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt
    77641 tcaatcaact ccagcactga cttcacgtaa atgcgggtcg gtgccagcag ataatcggct
    77701 aatggcttac catcaagttg ggtggtttgc ggatcacaac cgctgacttc aagaattttg
    77761 cgcaccagcg aatagccgtt cgagtgcgga ccgctggaac cgagtgcaat cagcacatcg
    77821 ccgtcgctga ctttagagcc gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa
    77881 cccgcgacat cgtaatcctc accgtgatac atccccggca tttctgccgt ttcgccaccc
    77941 accagtgaac agcccgattg cagacatcct tccgcgatac cgctgatcac cgctgaagcg
    78001 gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg ctctgcacct
    78061 tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt gtcgtgacgt
    78121 tttaagtcca ttgccagacg cagcttggta cctacgccgt cagtgccgga aaccagcacg
    78181 ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgcccag accgcccatc
    78241 acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag agcattaccc
    78301 gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt cactgcttgg
    78361 gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa cagggaaagc
    78421 aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttgccca ggcgcgcggc
    78481 gaaagaagac ttgtaccagg gtaaaggtta gttttcggat ggaataatct tctttcataa
    78541 ccatttgaat ataaaataac tttatctcaa gccgttatca ttttgactaa agtcaacgaa
    78601 aagaatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt gtggctgccc
    78661 ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc
    78721 tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc gcttccgaag
    78781 tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg
    78841 aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt accgttgtgc
    78901 caattctgcg tgccggtctg gggatgatgg acggtgtgct ggaaaatgtt ccgagcgcgc
    78961 gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct ggagccggta ccgtacttcc
    79021 agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgatcca atgctggcaa
    79081 ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc agcagcatca
    79141 aagttctggt gctggtagct gcgccagaag gtatcgctgc gctggaaaaa gcgcacccgg
    79201 acgtcgaact gtataccgca tcgattgatc agggactgaa cgagcacgga tacattattc
    79261 cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata aaaataatta
    79321 aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca taatacagag
    79381 gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact tttacagaca
    79441 atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc
    79501 ttatttcata ttaacccggc gactgtactg ttatttaacg gtattggaac gctgctgtat
    79561 ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc ctttatttca
    79621 ccggtattgt tactgttgcc gttagggtat gaagtcgcgc tgggcggctt tattatgtgc
    79681 ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg ctggctggac
    79741 gtgctgtttc cacctgcggc aatgggcgca atcgttgccg tcatcggtct ggagctggcg
    79801 ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc agactccaaa
    79861 accatcatca tctctattac caccctggcg gtcacggttt taggttccgt gctgtttcgt
    79921 ggtttcctgg caattatccc gattttaatt ggcgtgctgg tggggtacgc gctctctttc
    79981 gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc gctgccaacc
    80041 ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc ggcgttagtg
    80101 gttattgccg aacacgtagg gcacctggta gtaacggcta atatcgtcaa aaaagatctg
    80161 ctgcgcgatc caggtctgca ccgttcgatg tttgctaatg gcttgtcgac cgtgatttcc
    80221 ggcttctttg gctctacgcc aaataccact tacggagaaa acattggcgt gatggcgatc
    80281 acccgtgttt acagtacctg ggttatcggc ggggcggcga ttttcgctat cctgctttcc
    80341 tgcgtcggta aactggctgc cgctatccag atgatcccat tgccggtgat gggcggcgtt
    80401 tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat cgaatcgaaa
    80461 gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat catcggcgtc
    80521 agtggcgcga aggtaaacat cggcgcggcg gagttaaaag gtatggcgct ggcgaccatc
    80581 gtcggtatcg gcttaagttt gatcttcaaa ctgatcagtg tgctgcgccc tgaagaagtg
    80641 gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg ggcagtgttg
    80701 ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct
    80761 ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc ttcctgacga
    80821 cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggctg cgctgcaaaa
    80881 cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag gcgcggggcg
    80941 cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta
    81001 tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta tggagcattt
    81061 gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt gggagatggc
    81121 gattttcgat ctctacaatc gaattctgga atcgggcaaa acacgattgt tgatcaccgg
    81181 cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc gactcgactg
    81241 ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg cgctacagtt
    81301 acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg
    81361 gctcgacaga gaaatgcgca cgctatttat gacgttggat cagttggatc gtgcgtcgat
    81421 taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaagt tgtagataag
    81481 gtgtttattg tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt taatccatag
    81541 tattcgtagg ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg
    81601 atggagcaca tcttaaccca cgatttccag tacctgctcc ggtggacgac caatccgcgc
    81661 tttgccattc gcgaccacaa tcgggcgctc cattagcttc ggattatcca ccattgcctg
    81721 aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc agttctttat aaagatcctc
    81781 tttctggcgc atcaattcac gggcgctatt catccccagc attttcagca aatcgcgcag
    81841 cgttgccgca tcggcgggtg tctcaaggta gagtaccact tctggctcca cgccgttttc
    81901 tttcagcagg ttcagcgttt cccggctctt tgaacagcgt gggttatggt agatttttac
    81961 ctgtttggtc attgttcttc ctttaatgcg aattacatct tggtataagg cttaaagcgt
    82021 tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct
    82081 aatttcacct gcgaactggc gctactcaac agcgaaatgg cctgatcgag tcgtccggcg
    82141 agcgcataac cttctgctcg cgcagccagc tcctgatcgc ggttatttag cgcggcttcc
    82201 gcctgtgcca gtaaatccca gccgttgctg tcatctttat tattaaaggt gtagcgattc
    82261 agaatattgg ccgcttcttg tggttgaccg ccttgtagat aagcgttcgc caggttgagc
    82321 tgcaacaccg gattggtgcg caaatcacgg gcatttttca ggcgattgat tgcctcattg
    82381 gctttgtttt gcccaagatc gatatcggta gccagatcga gataccatgc gttgccaggt
    82441 tctgccgcca gtaacggttg cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc
    82501 gcctgtaaag cacgaccata ttgcgccgct cgttgctgac gaacgtttcc tttcgcccat
    82561 tcatccagca aatcactggt gagctggtta cgtccggaat tatacatccc cagtgtgcgc
    82621 gctttcgcca gatagaaatc ttccgacgac tgcaccacca tcggtcgcat ctgattagca
    82681 cggttgcggg catctgccag acgactttcc ggcaacgggt gagtcaataa aatttccggc
    82741 gggcgcgagg agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg catcgcctgc
    82801 ggatcgaatc ccgagcgttg cagcacctga ataccaatgc ggtccgcttc ctgttcattt
    82861 tgctgggtga aactgatcat tccctgacgt gttcccgcca gtgtaccggt cagcgccgcc
    82921 atccccgcct gcggactggc catcgccagt aaaatagaac ctaacgcgcc gacccaggtc
    82981 agcggcgcgc tgcgttgttg atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg
    83041 gagatttcgt gcgccataac tgaagccagt tgactttcgt tatcggaata acggaacagg
    83101 gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg
    83161 atcagaaaaa aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag acgcatcccc
    83221 agcgaattaa tatattgcgt taacagcggg tcattaatta acggcgcgct gccgcgtagc
    83281 tggcggacat aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg
    83341 gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca
    83401 atggtcatag cagcaatgag ggttgcaacc aggttttttt tcaactgcct gaacataacc
    83461 tctatcctgt atttctgaag atagtcattt gaccgatact gttaaatatc gttcccaccg
    83521 cgccgccttt gagtgtaacg gcgaagatta gcccactcaa atgacttttc agtttcctga
    83581 atccgtcata aaaagcgaag tcttttttgt gacatttcga tacaattgcg gatcgcaatc
    83641 ccgctcttga ggttcaggga aggtttttat gctcgaaatg ttgatgcaat ggtatcgccg
    83701 ccgttttagc gacccggaag cgattgcctt gctggttatt ttagttgccg gatttggcat
    83761 tatctttttc tttagtggcc tgcttgctcc gttgctggtg gctattgtgc tggcctattt
    83821 gctggaatgg ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct gggcgacgtc
    83881 gattgtattg gtggtttttg tcggtatatt gctactgatg gcgttcgtgg tactgcctat
    83941 cgcctggcaa cagggcatct acttaatccg cgatatgccg gggatgctca ataagctttc
    84001 tgactttgcc gccacgttgc cgcgccgcta tccggcgtta atggacgcgg gcattattga
    84061 tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat
    84121 ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc tatctggtgc tggtgccatt
    84181 gatggtcttc ttcctgctga aagacaaaga gcagatgctg aacgccgttc gtcgggtgct
    84241 gccgcgtaac cgtgggctgg caggacaggt gtggaaggag atgaatcaac aaatcaccaa
    84301 ctatatccgc ggcaaagtgc tggagatgat cgtggtaggg atcgccacct ggctggggtt
    84361 cttgctcttc gggctgaact attcgctgct gctggcggtg ctggtcggct tctcggttct
    84421 tattccgtac attggcgcat ttgtggtgac cattccggtg gttggcgtgg cgctattcca
    84481 gtttggcgca ggcacggaat tctggagctg cttcgcggtg tatctgatta ttcaggcgct
    84541 ggacggcaac ctgctagtac cggtgttgtt ctccgaagcg gttaacctgc atccgctggt
    84601 gattatttta tcggtggtga tcttcggtgg tttgtgggga ttctggggcg tattcttcgc
    84661 cattccattg gcgacgctga tcaaagccgt gatccacgcc tggcccgatg ggcaaatcgc
    84721 gcaagaataa tcttcagccc gacgaactgt ttcgccgggc tgatttttca ggattcctga
    84781 cgtaaataaa tagcccgata acacatactc accagcactg caccgccgat aatattaccc
    84841 agcatcaccg ggagcagatt ggcggtaata aaatggctga ccgtcagtgc cggaaaattg
    84901 tctgcgctac tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg aatggcaatg
    84961 gcgaacggaa tcacaaacaa attggcgata cagtgctcaa agccactggc gacaaacagg
    85021 gtgatgggca aaatcatggc gacgattttg tcgcataacg aacgcccgca ataactcatc
    85081 cacagcgcca ggcaaaccat cagattgcac ataatgccga ggctgacaga ttcagtaaat
    85141 gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca
    85201 ctcatcacca gcccggaaaa ccagattaac aaactgaaac aggcaatacc tgccagatta
    85261 ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc aactaataac gcccctactt
    85321 tttgccatta ccgtcattac cgacgaggtg aacaggttgg tgccgcaaac cgccagcaaa
    85381 ataaagccaa gtgtaaagca aaggccgccc accagatggg ttaatgcctg tgaagacggg
    85441 gcatcggcga taactgaaag gtaaaaagta aatccgatcg ccataaacac gccagcggac
    85501 atcgccatta aaaagaccga cactttactg cgggcaattt tatgggcggc aatccgttca
    85561 gcgattgccg cttttctggc gctcaactgc aagtcgaaag agagtttgtt tctcatgcat
    85621 actcctgcaa aagcagatta caacacctcg cgaaccgaga ttcccagacg ctgcattcgt
    85681 gacagcagcg tggtgcgctt catccctaat cgtgtcgccg cgccacgggg cccggcaaca
    85741 atgccattgg tttctcgcaa tacctgaaca atgcgctgac gttcttcttc gtcattctct
    85801 ggcgtcgtcg ggtgcagcaa ctgcgccatt gaactgcgaa gcgctgaatc ttcatttagc
    85861 gtcggcagta aacggctttg tcggacatta agatgtaaat tcagactgtt accgcgagtc
    85921 agcagtaccg ctcgctcaat cacgttttcc agctcgcgca cgttgcccgg ccagtcccac
    85981 gacatcaact ggcgtagcgc ctcggtcggg atggcgtcaa ttgaacgatt catatggcgc
    86041 gccatttttt gcgtgaaatg ctttgctaaa agagggatat cttccggacg gtcgcgcagc
    86101 ggcggcaatt ccagtgggaa gacattcagg cgataaaaga gatcgctgcg aaactggcga
    86161 tcttcaacca tttgccacaa atcacggttg gtggcggcaa tgactctgac atttaccggg
    86221 atcgttctac tcccgccgag acgctcaatc tcccgttcct gcaatacgcg cagcagttta
    86281 ggctgaagtt ctaacggcag atcgccaatt tcatcgagaa acaacgtgcc gccatcggca
    86341 atttcaaaac gaccacgatg ggtattaatc gcaccagtaa acgccccttt gtcatgaccg
    86401 aataactcac tttccagaag actggcgggg atggcagcgc agttgatttt gaccagcggc
    86461 ttgtcgcgtc gcgggctaag ttgatggatc gctctggcga tcacctcttt gccggttccg
    86521 gtttcaccgc aaatcaacac cgtactgtcg ctcttcgcca caatatctac ctgctggagt
    86581 aggtcttcca ttgcctggct ttgatagata atgtcaccga tgcccagatt cgaaaggagc
    86641 tgctcgctaa gctggtggtt ttcttgctgc aaactttcct gcaaatcggt catgctacgc
    86701 caggcatcgg cattgccaac ggcaatagcg atgcgatcgg ctatgtgttg tagtagctgg
    86761 cagttttcct cactaaagag agtggaagag gtatgcgcca gcaacaatgc acccggtgta
    86821 tggttgccaa aggtaagcgg tataaggagc gcagattcgc agccgttaga tgcaagtaag
    86881 agtaataacg ggtcgcgttg ccatagaaac agatcgtctg cacggtgcgt caaggtcggt
    86941 tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc caggcatatt gcgtggcaga
    87001 cacgcacaat gtgaggcaga aagatcgctg caccacaggc taaacttctc gttctttcga
    87061 tgatcgccca gtaccatact gactgaagcc agaccgaaaa aatgatggat ctcacgagcg
    87121 acgtcagcga tcagatcgtc gagatcaaga tgtgacagca ctgcattagt aatatcaacc
    87181 agtacccgat gatgatcgcg atctttctcc agcaaacgca gcgccacctg ctgttcttca
    87241 tcattgagcc atgattgcag aaccggggtg attatccgcg ccagccagtt aaacagtgtg
    87301 caatcctggg gagtaaaacg gctgccgtgg gtacgaataa agcgtagctc cgccattcca
    87361 ctactgccga gaaggacggc gtgacgcgaa taatcgacca tctcgccctg atactctgtt
    87421 gttgccgtca catcgctgcc atcatcatgg tagcggtaat agaagttgct tttgagaaca
    87481 tgcagttcaa taagttgtac cgggctgaac ggcggcggca tcgggatgaa ggcgcgtaat
    87541 aaagacgcct taccgtgttt ctgtgctaac cgctccgcga gcattccgtt taccgcttca
    87601 attgttattc cttgtggcgg ggcaaacatc gcctcgtctg acatagccat caacagtcct
    87661 tattaatgag aaatccttct cacgcaattt ttctggccat cagatagatg gccggttcct
    87721 gcacaatttc atccaacaga tgaatgaatt gaatgctacg cgcctgccac acagacgtcg
    87781 cgtcatccgc cagtggcgcg aatgcccggg tcaacagggc ggtgtggctg gcgtcaataa
    87841 caatctcacc aaaagtcatc acccccagca tcttacgtcg ggcttgctcc tcttcgacct
    87901 gggcaagcca atcttcgtat tccgtcagtg ggcaggggaa ggcgacattc agacaatcaa
    87961 tcacgccaac gtgatgaccg atagccagcg agtaatacat tacctgagag ctgtgttccg
    88021 gcatctcgtc gctactggcg acaaactttt ttcgcagcgt ccagaaaaca atttccccgc
    88081 actcttcagt catgtaaccc ccggagtacg cgaaagagtt gctgaacgat actattgaga
    88141 cgtggatcgt cggcatcgcg caaccaggtg ttaacccgat ttcctgtagg atcgtcgctt
    88201 aaatggcgca ggagccgatc gcaaatttct cgcccctgac gatagcccgc cagccgccgc
    88261 gcttctcgct caatggcgat acgctgtgat ggcgggatct gcggccacaa cggttgcata
    88321 gtcaccccgg tgggatcgcg ataatccacc gcgtgaatct tctgttgcag caaaccgagc
    88381 gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg ggcagccggg gatccaaaca
    88441 tcaatgggga caatggtgtc gctgccgccc cagacgctgt agagatcgtg gaaaatccgc
    88501 cgccggcacc gcacgcgccg taggaaacac agattttatg atcgggggca gactcatacg
    88561 cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc tgtaaataac aaaatatcgg
    88621 cgtgacgcgg tgatgaaata accttaatgc caaaacgttc tgcgtcgaat actggtgtga
    88681 tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc gcaatcgacg cgataaacgt
    88741 aagccgagcg acggatatcc tgtagcaaat gctgcttcat cttttgcgtt tgctcgtcca
    88801 gcgtgatggg ctggctgaca tgttgtgtaa gcactggact catagctgct ccttagccac
    88861 cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt tgtttgcact ccgggcagac
    88921 gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca ttttgttgct gtgccaacaa
    88981 ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg cgttcgcaac ggctgcaacg
    89041 ttgtagatgg aacgtcgcgc gggtatagag atcggcttta ttggtgacgg tcagttcaaa
    89101 gttattggta agctggatgg ctctggtcgg gcacacttct tcacaacgtc cgcagtaaat
    89161 acaacgcctc agatagagct gccaggtgcg cgaattttgc tggtcgtcgg tctggatagt
    89221 cagcgcattt gccggacaag cacaggcgca ggcaccgcag gcaatacatt ggctgggcat
    89281 caggtccggt ttcccgcgaa agcctgggct gacctccagt ggcgcgaagg gatatttcac
    89341 cgtcgcggtt ccggcgcgca taatagtttt cagtaacttc agcatcttct gtccttactt
    89401 cagcggcgaa cggttacgat caataccgta gcgttcgatc tctttatacg gcacggtttt
    89461 tgactggcgc ttgcgcacat ctaccagcgt cacacggtcg gtacaggagt agcagggatc
    89521 aaggctaccg ataatcagcg gtgcgtcaga aacggtattg ccgcgcagca tgtaacgcaa
    89581 caccggccag ttggcgtagg tggcggcacg gcagcgccag cggaacaatt tttggttatc
    89641 accgagcatg ctccagtgca cgtcttcacc gcgtggcgct tcaacaaagc ccagcgcgaa
    89701 tgcgtgaggt ttatagctaa agccttcggt cagcagtggg gtatccggca tgttgtcgag
    89761 ggcaaattcc agcattgcca gcgaatcaaa cgtctctttg acacggacca tcacgcggga
    89821 gaaaacatcg ccgccggtaa aggtaaacag tgtttttgga atattgccgt agtcggcgta
    89881 ggggtgatca aagcgcaaat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
    89941 gcgttgttcc atattcggcg tagcaagcag catctctacc agctccgaca cgtcggcgcg
    90001 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
    90061 accaccaatc agattcagac cgtaggtttt acgcgacccg atcagcaatt ccgccatcgt
    90121 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaaac caacgaaatg
    90181 gcaggagagg ccaaggttaa gcaaatgact gtgtagccgt tcgacttcca gcagaatcga
    90241 gcgaatagta tgtgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
    90301 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cggtccgata agaaggtcac
    90361 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
    90421 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccagttc
    90481 atcggaggtg atatgcagcg ggccgacagg gatcacctgc gcatcgctgt tgccctcatt
    90541 gataaacgga tacgtttcgg aatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
    90601 atctttgcgc agcggatgca tatcttccgg ccagtcatcg ggcaacacca ggcgacgctg
    90661 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
    90721 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
    90781 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
    90841 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
    90901 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
    90961 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
    91021 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
    91081 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
    91141 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
    91201 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
    91261 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
    91321 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
    91381 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
    91441 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
    91501 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
    91561 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa ggtgtgcagc agcgcggcaa
    91621 aaattcccaa tggcccgcca atgcctagcg ccaccgcgac cagtcccatg ttctccacgc
    91681 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
    91741 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggcct
    91801 ggcagataat gatgtaatag cgaattagca ccaacagcgc gcagttcagc aatacggcgg
    91861 agagcagggc gctgaccgga ctcggtgctt cactgtgagc atccggcagc caggcgtgca
    91921 tgggaaatag cccggttttg gtgccaaagc caattagcac aaacacaaag gccaacagca
    91981 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
    92041 ctgcctgcgg cataacgctg gcgacgttgg cgtataccag cacggtaccg aacagaccaa
    92101 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgcttccagc gatgaacgct
    92161 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
    92221 tcacaatcag gttattgctg gtaacaacca gcagcatggt aaacaaaaac agatggaaga
    92281 aaccgtagta atcgcacagc gtaacggggg aaagctcgcc gtgtgccact tcatgacgca
    92341 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
    92401 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
    92461 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
    92521 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
    92581 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
    92641 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
    92701 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
    92761 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
    92821 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
    92881 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
    92941 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
    93001 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
    93061 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
    93121 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
    93181 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
    93241 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
    93301 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
    93361 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
    93421 caggcacaac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
    93481 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccggcag
    93541 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacgccggta atcgccagcg
    93601 ctgccacgca gaaacccacg cctggcaacg gcagggtgtg caatacgcca cgcagacgcg
    93661 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
    93721 tagcgaacgc gtggttgacg atgtaggcga tgctgccctc cagcgccagc cgcgagccga
    93781 agatggagag cgacaaaccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
    93841 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
    93901 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
    93961 cgcgggcaaa aatgtaaacg cccactttca ccttcgatgc ggcgtggaga taggcactga
    94021 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
    94081 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
    94141 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
    94201 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa cgctgtgctt
    94261 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
    94321 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacaaca
    94381 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
    94441 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
    94501 gcgtactcac gcgatcaatg accaggccaa acaatgccac atcccccacg ttgaccaatg
    94561 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagtgac aacatgcaca
    94621 gcgtggtcag cgcggcgaac aaaacccccc attcggcggc ccgacgttgt ggcgaaaacg
    94681 aaacgaccag tgcgccgata aaaggcagca ataacgtcgt cagagcaaga ttttccatat
    94741 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
    94801 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
    94861 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    94921 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg acagaaacag ggccacaaac
    94981 agtgatgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    95041 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    95101 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    95161 gttagcggcg agttccagcc catcgccagc gtattgctga tcatctcgat atgcgtggaa
    95221 cctgctatca gcgccaatac cagcagtgag agaataagca ttggttcgac cagaatgccg
    95281 agcgtcaact cgcgactggc accgactccc gcaaacggac ttccggtatc cagcccggaa
    95341 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    95401 cccgcaaaag gggaaacggt aataaacagt ggtaaggcca tcgccagcac cagcatgctg
    95461 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcaacttcc
    95521 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    95581 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    95641 agaatcacgg cctgtgctaa cgcaaaaata accagatatc cgtcgcaaag agtttgtctc
    95701 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    95761 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    95821 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    95881 cctggcggtg acgtgcgcaa aacctttatt tagcctcagc gtagggtcga gctgttgtcg
    95941 tagacgaaat aacgcagaga agaccacacg cagcggctgc atcacgccat tgcctgacgg
    96001 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    96061 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    96121 taacagcaac agtaatgagg gggtgacctg tgtatgaaac gtgccgggta caagtgctat
    96181 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatat gcattatctt
    96241 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    96301 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtac tgcgcggcgc
    96361 accacagaaa gtaataccgt acatttttac gaagcacatt actgccagcc caccagtgac
    96421 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    96481 tagtgagaac agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    96541 ggctgatatc gcgaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    96601 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cgcccgcgcc
    96661 gagaaatagc agccctttga acagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    96721 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgactgc
    96781 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    96841 ttccgccagc gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    96901 aatgccccac cacagcggca aacccgtttg cgccagcagg tccatcgcta ctttcaggat
    96961 gccgaaaata ccgattttga ccattacgcc agacatcaac gccgaagcgt gcgatggtgc
    97021 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    97081 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    97141 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat
    97201 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    97261 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    97321 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat
    97381 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    97441 gagcgaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    97501 cattcgcagg gtaaaatcgg caaagtcaaa aggggtggca aaacgggcga ctaaagttac
    97561 accagcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt
    97621 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    97681 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    97741 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    97801 tgtttttctt tcatctgtcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    97861 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    97921 gcgacggtct ggacacccgg ttcccagcgc agcaatgcat gttggggcgg cacgttttct
    97981 tcgccgtctt tgagtgagcc ttcagcctga aaaacatatt gcgcaggggc attcaccgga
    98041 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    98101 atacagaggc tttcgttgag ttggatcgca tcatccctct gggagatggc attaaccggg
    98161 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    98221 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    98281 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    98341 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    98401 tgccattctg attagctcaa tatattcaat aagttatgta ttgactggat tctgaacggt
    98461 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    98521 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    98581 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    98641 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    98701 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    98761 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    98821 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    98881 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    98941 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    99001 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    99061 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    99121 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    99181 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    99241 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    99301 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    99361 atgctggcat tggcggacgc ggacgcggac gcggacgcgg acgcgccgtc gtgcgcttca
    99421 tcacgattaa aagatccagt tcggcacctt tcaacggtaa cgttgattca atcagagtga
    99481 tggcattcca tgaaccggaa agcagcatga taaacgtgaa ctcttctccc agcatcgcca
    99541 ggcgactgtc ttcaatatta cagccgcaac tactgacatg acgggtgatg gtgttcacaa
    99601 ttccagggcg atcggcaccc aacgcagtga tcaccagata atgttgcgat gacagtgtca
    99661 aactggttat tcctttaagg ggtgagttgt tcttaaggaa agcataaaaa aaacatgcat
    99721 acaacaatca gaacggttct gtctgcttgc ttttaatgcc ataccaaacg taccattgag
    99781 acacttgttt gcacagagga tggcccatgt tcacgggaag tattgtcgcg attgttactc
    99841 cgatggatga aaaaggtaat gtctgtcggg ctagcttgaa aaaactgatt gattatcatg
    99901 tcgccagcgg tacttcggcg atcgtttctg ttggcaccac tggcgagtcc gctaccttaa
    99961 atcatgacga acatgctgat gtggtgatga tgacgctgga tctggctgat gggcgcattc
   100021 cggtaattgc cgggaccggc gctaacgcta ctgcggaagc cattagcctg acgcagcgct
   100081 tcaatgacag tggtatcgtc ggctgcctga cggtaacccc ttactacaat cgtccgtcgc
   100141 aagaaggttt gtatcagcat ttcaaagcca tcgctgagca tactgacctg ccgcaaattc
   100201 tgtataatgt gccgtcccgt actggctgcg atctgctccc ggaaacggtg ggccgtctgg
   100261 cgaaagtaaa aaatattatc ggaatcaaag aggcaacagg gaacttaacg cgtgtaaacc
   100321 agatcaaaga gctggtttca gatgattttg ttctgctgag cggcgatgat gcgagcgcgc
   100381 tggacttcat gcaattgggc ggtcatgggg ttatttccgt tacggctaac gtcgcagcgc
   100441 gtgatatggc ccagatgtgc aaactggcag cagaagggca ttttgccgag gcacgcgtta
   100501 ttaatcagcg tctgatgcca ttacacaaca aactatttgt cgaacccaat ccaatcccgg
   100561 tgaaatgggc atgtaaggaa ctgggtcttg tggcgaccga tacgctgcgc ctgccaatga
   100621 caccaatcac cgacagtggt cgtgagacgg tcagagcggc gcttaagcat gccggtttgc
   100681 tgtaaagttt agggagattt gatggcttac tctgttcaaa agtcgcgcct ggcaaaggtt
   100741 gcgggtgttt cgcttgtttt attactcgct gcctgtagtt ctgactcacg ctataagcgt
   100801 caggtcagtg gtgatgaagc ctacctggaa gcggcaccgc ttgcggagct tcatgccccg
   100861 gctggaatga ttttgccggt gacctccggt gattatgcaa tcccggtgac caacggtagt
   100921 ggtgctgtcg gtaaggcgct ggacattcgt ccaccagccc agccgctggc actggtttct
   100981 ggtgcgcgta cccagttcac gggcgatacc gcttcattgc tggtggaaaa tggtcgtggc
   101041 aatactctgt ggccgcaggt ggttagcgtg ctgcaggcga aaaactacac catcacccaa
   101101 cgtgatgatg ctggtcagac actgaccacc gattgggtac aatggaaccg tctggacgaa
   101161 gacgagcagt atcgtggtcg ttatcaaatc tctgttaagc cgcagggtta tcagcaggcg
   101221 gttacggtta aactgctgaa cctggaacag gcgggcaaac cggttgcaga cgcggcttcc
   101281 atgcagcgtt acagcacgga gatgatgaac gttatttccg ccggtctgga taaatctgcc
   101341 actgacgccg cgaacgctgc gcaaaatcgt gcctccacca ctatggacgt acaaagtgca
   101401 gctgatgaca ccggtttacc aatgctggtc gtacgcgggc cgttcaatgt ggtttggcaa
   101461 cgtctgccag cggcgctgga aaaagtgggc atgaaagtga ccgacagcac ccgttcgcag
   101521 ggcaacatgg ccgtaactta taagccgctg tctgacagcg actggcagga actgggcgcg
   101581 agcgatccag gcctggcatc cggtgactat aaactgcagg ttggcgattt agataaccgc
   101641 agcagcctac agttcatcga tccgaaaggt catactctga ctcagagtca gaacgacgcg
   101701 ctggtagctg tcttccaggc tgcgtttagc aagtaaaaat acagggctgg aatcatccgg
   101761 cccttttttc tgatatgata cgcaaacgtg tgcgtcggca ggaaaacgcg attttagcgg
   101821 taattcgcac gaaatttgtt tgtcggacgt agttcggata aggcgttcac gccgcatccg
   101881 acaaaacatc cggcacacca gacagcaaaa gattttaaaa cgttaattca cacccaggag
   101941 tgataaagat gcaaaagcaa gctgagttgt atcgtggtaa agcgaaaacc gtatacagca
   102001 cggaaaaccc ggacctgttg gtgctcgaat tccgcaatga tacgtcagca ggggatggtg
   102061 cgcgcattga gcagtttgat cgcaaaggta tggtgaacaa caagttcaat tacttcatta
   102121 tgagcaaact ggctgaagcg ggtatcccga ctcaaatgga gcgtctgctc tccgataccg
   102181 aatgtctggt gaaaaagctg gatatggtgc cggttgagtg tgtcgtgcgt aaccgtgctg
   102241 ctggctctct ggtgaaacgt cttggaatcg aagaaggtat tgagctgaac ccgccgctgt
   102301 tcgatctgtt cctgaaaaac gacgccatgc acgatccgat ggtcaacgaa tcttactgcg
   102361 aaacctttgg ctgggtgagc aaagagaacc tggcgcgtat gaaagagctg acctacaaag
   102421 cgaacgacgt gctgaaaaaa ctgttcgatg atgctggtct gattctggtc gacttcaagc
   102481 tggagtttgg cctgtacaaa ggtgaagtgg tactgggcga tgagttctcc ccggacggca
   102541 gccgcctgtg ggacaaagaa acactggaga aaatggacaa agaccgtttc cgccagagcc
   102601 tcggtggcct gatcgaagcc tatgaagccg tcgcccgccg cctgggtgta cagctggact
   102661 gatttttctg gttgcttgtt tctttagccg gagggtactc cggctaaatg tctttgatac
   102721 ccccgctaca atacttctca ttatctccct ttgacaggaa agtcatacca ggccaatatg
   102781 tatatacata acgtatatcc caaaaagggg gggctatgga aactactgta tttttaagta
   102841 atcgcagtca ggccgtcaga ttaccgaaag cggtcgctct gccagaagac gttaagaaag
   102901 tagaaattat tgcgattggt cgtacaagaa tcatcactcc cgccggtgag tcctgggaca
   102961 gttggtttga tggcgaaaat gtgagtgcgg attttatgga tatccgtgat caacccgcaa
   103021 tgcaggaaag ggaatcattt tgatgctgaa attcatgctg gataccaata tctgtatttt
   103081 cactattaag aataaaccag tacatgtcag agagcggttt aacctgaatt ctggccggat
   103141 gtgtataagt tctgtaacat tgatggagct tatctatggg gcagaaaaaa gtcagatgcc
   103201 agaacgcaat ttggcggtta ttgaaggttt cgtatctcgt ttggtagtgc ttgattatga
   103261 tagtgctgct gcgacgcata ccgggcaaat cagggccgag cttgcccggc agggaaggcc
   103321 tgttggacct tttgaccaga tgatagcggg acatgctcgc agtcgcggtt tgattgtggt
   103381 taccaacaat acccgagagt ttgagcgagt ggcgggaatc agactagaag actggagcta
   103441 acattctctt ttacttctaa cattcgtaaa ttatataacg tggcatgaag ccttaattcc
   103501 tccgccattt catcccgctt tcctgtggta atcttcctct gaaccaccta cgatcatttc
   103561 tataatgaat atattgttga ggtatctatg cgttggcaag ggcgacgtga aagtgacaat
   103621 gttgaagaca ggcgcaacag ctccggtggt ccgtctatgg gcggtcccgg ttttcgcctg
   103681 ccaagcggta aaggcgggct gattttactg ttagtcgtgc tggttgcagg ctactatggt
   103741 gttgatttaa ccgggttaat gactggacag ccggtttccc aacaacaatc aacgcggtcc
   103801 attagcccta atgaagacga agctgcaaaa ttcacctcgg tgattctggc aaccacggaa
   103861 gacacctggg gacaacagtt cgagaagatg ggtaagacct atcagcaacc gaagctggtc
   103921 atgtaccgtg gaatgacgcg caccggctgc ggggctggtc agtccattat ggggccgttc
   103981 tattgcccgg cggatggcac agtttatatc gatctctcct tctatgatga catgaaagac
   104041 aaacttggcg cggatggcga ttttgcccag gggtacgtta tcgcccatga agtcggtcat
   104101 catgtgcaga aactgttagg catcgagccg aaagttcgtc aactgcaaca aaacgcgacg
   104161 caggcggaag tgaaccgctt atctgtgcgt atggaactcc aggccgactg ttttgccggt
   104221 gtctgggggc atagtatgca gcagcaaggc gttctggaaa ccggcgatct ggaagaggcg
   104281 ctgaacgcgg cgcaggccat cggcgatgac cgtttacaac agcaaagtca ggggcgagta
   104341 gtaccagata gtttcacaca tggcacttct cagcaacgct acagctggtt taaacgtggt
   104401 ttcgacagcg gcgatccggc acaatgcaat acttttggta aaagcattta acttccgggg
   104461 cagggatggc tgaactgact gcgcttcaca cattaacagc gcaaatgaaa cgtgaaggga
   104521 tccgccgctt gctggtgttg agcggggaag agggttggtg ttttgatcat gcgcttaagt
   104581 tgcgtgatgc cttacctggc gactggctgt ggatttcgcc gcagccagat gctgaaaacc
   104641 actgttcacc ctcggcgcta caaactttac ttgggcgcga gttccggcat gcggtattcg
   104701 acgctcgcca cggctttgat gccgctgcct ttgcagcact tagcggaacg ttgaaagcgg
   104761 gaagctggct ggttttgtta ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg
   104821 actcgctgcg ctggagtgat tgccctgacc ctattgcgac gccgcatttt gtccagcatc
   104881 tcaaacgcgt acttacggcg gataacgacg ctatcctctg gcggcaaaac cagccgttct
   104941 cgttggcgca ttttactccc cgtactgact ggcaccccgc gaccggcgca ccacaaccag
   105001 aacaacagca actcttacag cagctactga ccatgcctcc gggcgtggca gcggtaacgg
   105061 ctgcgcgtgg gcgcggtaag tcggcgttgg cagggcaact catttctcgt attgcgggca
   105121 gtgcgattgt caccgcgccc gcaaaagcgg caacggatgt actggcacaa tttgcgggcg
   105181 agaagtttcg ctttattgca ccggatgcct tgttagccag cgatgagcaa gccgactggc
   105241 tggtggtcga cgaagccgca gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt
   105301 ttcctcgaac gttgttaacc actacggtgc agggctacga aggcaccgga cgtggttttt
   105361 tgctgaaatt ttgcgctcgc tttccgcatt tacaccgttt tgaactgcaa cagccgatcc
   105421 gctgggcaca gggatgcccg ctggaaaaaa tggttagtga ggcactggtt tttgacgatg
   105481 aaaacttcac ccatacacca caaggcaata tcgtcatttc cgcatttgaa caaacgttat
   105541 ggcgaagcga gccagaaacg ccgttaaagg tttatcagtt attgtctggt gcgcactacc
   105601 ggacttcgcc gctggattta cgccggatga tggatgcacc agggcaacat tttttacagg
   105661 cggctggcga aaacgagatt gccggagcgc tgtggctggt ggatgagggg ggattatctc
   105721 aacaactcag tcaggcggta tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc
   105781 agtcgctggc ggcgcacggt agcaatccac tggcagcgac attgcgtgga cggcgggtca
   105841 gccggatagc agttcatccg gcgcgtcagc gggaaggcac agggcagcaa cttattgctg
   105901 gtgctttgca atatacgcgt gacctcgact atctttcggt gagttttggt tacaccgggg
   105961 agttatggcg tttctggcac cgctgcggtt ttgtgctggt gcggatgggt aatcatcgtg
   106021 aagccagcag cggttgctat acggcgatgg cgctgttacc gatgagtaat gcgggtaaac
   106081 aactggctga acgtgagcat taccgtttac gtcgcgatgc gcaagctctc gcaaagtgga
   106141 atggcgaaac gcttcctgtt gatccactaa acgatgccgt cctttctgac gacgactggc
   106201 ttgaactggc cggttttgct ttcgctcatc gtccgctatt aacatcgtta ggttgcttat
   106261 tgcgtctgct acaaaccagt gaactggcat taccggcgct gcgtgggcgt ttacagaaaa
   106321 acgtcagcga cgcgcagtta tgtaccacac ttaaactttc aggccgcaag atgttactgg
   106381 tccgtcagcg ggaagaggcc gcgcaggcgc tgttcgcact taatgatgtt cgcactgagc
   106441 gtctgcgcga tcgcataacg caatggcaat tttttcactg actccttcaa ttttctccca
   106501 tggtcattgt gagtcagcgg cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa
   106561 catgaccatt ttgttgttca aagcccggat aaacctgcgc aacagttgtt actgcttttc
   106621 cacggtgtcg gggataaccc ggtggcaatg ggggaaatag gtagctggtt tgcaccgctg
   106681 tttcctgatg cgctggtggt gagtgtcggt ggcgcggagc cgagcggtaa tccggcgggg
   106741 cgtcagtggt tttcagtgca gggtattacg gaagataatc gccaggcgcg tgtggatgcc
   106801 atcatgccga cgtttattga aacggtacgc tactggcaga aacagagcgg agtaggggct
   106861 aacgccacgg cgctaattgg tttctcacaa ggcgcgatta tggcgctgga gagcattaaa
   106921 gccgaaccgg gccttgcttc gcgcgtcatt gcctttaacg gacgctatgc gagcctgccg
   106981 gagaccgcgt ctacagccac gacaattcac ttgattcatg gtggtgaaga tccggttatc
   107041 gatctggcgc atgcagttgc ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg
   107101 ctggatatcg tggaggattt aggccatgcg atcgacaatc gcagtatgca atttgccctg
   107161 gatcacttgc gttataccat tccgaaacat tatttcgacg aagcgctaag tggcggtaag
   107221 cctggcgatg atgatgtgat tgaaatgatg taaaagacag attacttggc ctgataagac
   107281 gcgtgagcgt cgcatcaggc atcgttcacc attgccggat gcggcgttaa cgccttatcc
   107341 ggcctacggt tgagcactca gcttacttct tcggttgatc ctttttcggc cagtcgtcgt
   107401 catcgtccca cttatcgttg aaatcacgat gcgggggaag ctccggcttg ttggcgagaa
   107461 atttcttatg gtcgacgcgc ttgagatctt taatcacgtt cagcaggacg cctaccaaaa
   107521 agacaatcac cagaatccac caatatttag ccagccagtc catgcttatt tcctcttgca
   107581 gaaccactca tcaggcgacg agctgttcca tgatacgttg atacatacgg gcaagtagct
   107641 gcaggtcggc agcgttcaca cattcattaa ttttatgaat agtggcattg accggcccga
   107701 gttccaccac ctgcgccccc atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg
   107761 tcagtagctg cggtttaatt tcattatagt gctcaaccgc gttaacgacc gcatccacca
   107821 gtttaccgcg cgcggtcaaa aatggctgcc cggaaagcca ccagtccacc gtataacgca
   107881 gttgatgttt ttcaagcagg gcaagcacct gtgctttgat catctcatca gtcagttcgg
   107941 tgctgaagcg gaagttaaac tgcacaaaca gttcacccgg aataacgttg ttactgcccg
   108001 ttcccgcctg aatattggca atctgcatac tggtcgccgg gaagaattca ttgccctgat
   108061 cccactcaat agccactaat tcattaagga aaggtgctgc gcgatgtacc ggattgtcag
   108121 ccagatgcgg gtaggcaaca tgcccctgaa cgccatgaat ggtgaggttg caggttaacg
   108181 atccgcgacg accatttttc accacatcac ctaccacttc gatactcgac ggttcgccga
   108241 ccaggcagta atcgagacgc tcattacgtg ccattaacgc ttcgacgact tttaccgtac
   108301 cgttgtgtgc actggcttct tcatcagagg tgatcagaaa tgccagtcgc cccgtatggt
   108361 tgggatgttg tgcgacaaag cgttctgccg ctaccaccat cgccgccagc gagcctttca
   108421 tatctgccgc accgcgcccg aataacatgc catcacgaat ggtgggttca aacggcggat
   108481 tgatccaacg atcggcgtcg ccaggcggca ccacgtcggt atgcccggca aaggctaacg
   108541 tttcaccttg cccacgccat gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa
   108601 cggtaaaacc gatcgcctgc aaacgttcaa tcaacaaagc ctggcatcct gcgtcatcag
   108661 gactcaggga agggcggcga ataagctgtt gtgtcagctc aataaccggg cacgacatag
   108721 actacacctc atggaaaaat tgctgataac tggaatcact gaaacccagc agcataggct
   108781 taccaggcgc gcagagcaat ggacgtttga taattgcagg catttcagtc attaatgccg
   108841 ccgcagaggc cgcatcggtg attttattgc gggtggtttc gtccagttta cgccaggttg
   108901 taccacgggt gttgagtaac gcttcccagc ctaattcgtt gataaaaccg ttcaataatt
   108961 cgctgtccag cccatcgacg cggtaatcat gaaaacgata gtcgatgtta ttggcttcca
   109021 gccaacggcg agccttttta atggtgtcac aatttttgat gccgtaaagt gtaaccataa
   109081 tgaatccttt ttcgatttat gagaataaat actcatttaa gggtgaaaac attttgcctg
   109141 caaatgatta tcaga
//
LOCUS       DAZGTR010000003        98510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGTR010000003 DAZGTR010000000
VERSION     DAZGTR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 98510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.003"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(94..375)
                     /gene="yehE"
                     /locus_tag="ACHLCY_000202"
     CDS             complement(94..375)
                     /gene="yehE"
                     /locus_tag="ACHLCY_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP0367534.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(638..1747)
                     /gene="apbC"
                     /locus_tag="ACHLCY_000203"
     CDS             complement(638..1747)
                     /gene="apbC"
                     /locus_tag="ACHLCY_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP0367535.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1879..3912
                     /gene="metG"
                     /locus_tag="ACHLCY_000204"
     CDS             1879..3912
                     /gene="metG"
                     /locus_tag="ACHLCY_000204"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP0367536.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4053..7847
                     /locus_tag="ACHLCY_000205"
                     /pseudo
     CDS             4053..7847
                     /locus_tag="ACHLCY_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7857..11489
                     /gene="yehI"
                     /locus_tag="ACHLCY_000206"
     CDS             7857..11489
                     /gene="yehI"
                     /locus_tag="ACHLCY_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP0367537.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRRWPGEPVSVITGSLIQ
                     TLGDELLQQAGQKENITWNYDKCSLEWQSAIQQAINLTGEHKPSIPALTMAALICIAQ
                     NDSQQLLDEIVQQEGLEYATDVVIARQCIARRYESDSLVVTLQYQDEDYGYGYGSATY
                     NDFDLRLRKHLSLAEESCWQRCADKLIAALPGIPKIRRPFIALILPEKPEIANELASL
                     ESSRSSLHSKEWLKVVATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQEGLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIGLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVHRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11551..11868
                     /gene="yehK"
                     /locus_tag="ACHLCY_000207"
     CDS             11551..11868
                     /gene="yehK"
                     /locus_tag="ACHLCY_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP0367538.1"
                     /translation="MIVQKELVAIYDYEIPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDTDPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            13108..14196
                     /gene="yehL"
                     /locus_tag="ACHLCY_000208"
     CDS             13108..14196
                     /gene="yehL"
                     /locus_tag="ACHLCY_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP0367539.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            14207..16486
                     /gene="yehM"
                     /locus_tag="ACHLCY_000209"
     CDS             14207..16486
                     /gene="yehM"
                     /locus_tag="ACHLCY_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFP0367540.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            16479..17615
                     /gene="yehP"
                     /locus_tag="ACHLCY_000210"
     CDS             16479..17615
                     /gene="yehP"
                     /locus_tag="ACHLCY_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0367541.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17612..19610
                     /gene="yehQ"
                     /locus_tag="ACHLCY_000211"
                     /pseudo
     CDS             17612..19610
                     /gene="yehQ"
                     /locus_tag="ACHLCY_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
     gene            19735..20196
                     /gene="yehR"
                     /locus_tag="ACHLCY_000212"
     CDS             19735..20196
                     /gene="yehR"
                     /locus_tag="ACHLCY_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP0367542.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGTTTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(20236..20706)
                     /gene="yehS"
                     /locus_tag="ACHLCY_000213"
     CDS             complement(20236..20706)
                     /gene="yehS"
                     /locus_tag="ACHLCY_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP0367543.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIFLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(20753..21472)
                     /gene="btsR"
                     /locus_tag="ACHLCY_000214"
                     /gene_synonym="yehT"
     CDS             complement(20753..21472)
                     /gene="btsR"
                     /locus_tag="ACHLCY_000214"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP0367544.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(21469..23154)
                     /gene="btsS"
                     /locus_tag="ACHLCY_000215"
     CDS             complement(21469..23154)
                     /gene="btsS"
                     /locus_tag="ACHLCY_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP0367545.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            23376..24107
                     /gene="mlrA"
                     /locus_tag="ACHLCY_000216"
     CDS             23376..24107
                     /gene="mlrA"
                     /locus_tag="ACHLCY_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP0367546.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            24167..24274
                     /gene="yohO"
                     /locus_tag="ACHLCY_000217"
     CDS             24167..24274
                     /gene="yohO"
                     /locus_tag="ACHLCY_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP0367547.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(24255..24986)
                     /gene="yehW"
                     /locus_tag="ACHLCY_000218"
     CDS             complement(24255..24986)
                     /gene="yehW"
                     /locus_tag="ACHLCY_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP0367548.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(24991..25917)
                     /gene="yehX"
                     /locus_tag="ACHLCY_000219"
     CDS             complement(24991..25917)
                     /gene="yehX"
                     /locus_tag="ACHLCY_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP0367549.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTSL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(25910..27067)
                     /gene="yehY"
                     /locus_tag="ACHLCY_000220"
     CDS             complement(25910..27067)
                     /gene="yehY"
                     /locus_tag="ACHLCY_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP0367550.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(27074..27991)
                     /gene="osmF"
                     /locus_tag="ACHLCY_000221"
     CDS             complement(27074..27991)
                     /gene="osmF"
                     /locus_tag="ACHLCY_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP0367551.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGSVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(28202..30499)
                     /gene="bglX"
                     /locus_tag="ACHLCY_000222"
     CDS             complement(28202..30499)
                     /gene="bglX"
                     /locus_tag="ACHLCY_000222"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP0367552.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            30695..32410
                     /gene="dld"
                     /locus_tag="ACHLCY_000223"
     CDS             30695..32410
                     /gene="dld"
                     /locus_tag="ACHLCY_000223"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0367553.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(32448..33380)
                     /gene="pbpG"
                     /locus_tag="ACHLCY_000224"
     CDS             complement(32448..33380)
                     /gene="pbpG"
                     /locus_tag="ACHLCY_000224"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP0367554.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(33554..34141)
                     /gene="yohC"
                     /locus_tag="ACHLCY_000225"
     CDS             complement(33554..34141)
                     /gene="yohC"
                     /locus_tag="ACHLCY_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP0367555.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            34311..34889
                     /gene="yohD"
                     /locus_tag="ACHLCY_000226"
     CDS             34311..34889
                     /gene="yohD"
                     /locus_tag="ACHLCY_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0367556.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(35019..35780)
                     /gene="yohF"
                     /locus_tag="ACHLCY_000227"
     CDS             complement(35019..35780)
                     /gene="yohF"
                     /locus_tag="ACHLCY_000227"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0367557.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATYEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(35833..37269)
                     /gene="mdtQ"
                     /locus_tag="ACHLCY_000228"
     CDS             complement(35833..37269)
                     /gene="mdtQ"
                     /locus_tag="ACHLCY_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP0367558.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCGPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            37493..37576
                     /gene="yohP"
                     /locus_tag="ACHLCY_000229"
     CDS             37493..37576
                     /gene="yohP"
                     /locus_tag="ACHLCY_000229"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP0367559.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(37964..38914)
                     /gene="dusC"
                     /locus_tag="ACHLCY_000230"
     CDS             complement(37964..38914)
                     /gene="dusC"
                     /locus_tag="ACHLCY_000230"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP0367560.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            39153..39551
                     /gene="yohJ"
                     /locus_tag="ACHLCY_000231"
     CDS             39153..39551
                     /gene="yohJ"
                     /locus_tag="ACHLCY_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP0367561.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            39548..40243
                     /gene="yohK"
                     /locus_tag="ACHLCY_000232"
     CDS             39548..40243
                     /gene="yohK"
                     /locus_tag="ACHLCY_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP0367562.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNVMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            40373..41257
                     /gene="cdd"
                     /locus_tag="ACHLCY_000233"
     CDS             40373..41257
                     /gene="cdd"
                     /locus_tag="ACHLCY_000233"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP0367563.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            41407..42126
                     /gene="sanA"
                     /locus_tag="ACHLCY_000234"
     CDS             41407..42126
                     /gene="sanA"
                     /locus_tag="ACHLCY_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP0367564.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            42129..42368
                     /gene="yeiS"
                     /locus_tag="ACHLCY_000235"
     CDS             42129..42368
                     /gene="yeiS"
                     /locus_tag="ACHLCY_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP0367565.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            42687..43925
                     /gene="preT"
                     /locus_tag="ACHLCY_000236"
     CDS             42687..43925
                     /gene="preT"
                     /locus_tag="ACHLCY_000236"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP0367566.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            43919..45154
                     /gene="preA"
                     /locus_tag="ACHLCY_000237"
     CDS             43919..45154
                     /gene="preA"
                     /locus_tag="ACHLCY_000237"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP0367567.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(45225..46235)
                     /gene="mglC"
                     /locus_tag="ACHLCY_000238"
     CDS             complement(45225..46235)
                     /gene="mglC"
                     /locus_tag="ACHLCY_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP0367568.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(46251..47771)
                     /gene="mglA"
                     /locus_tag="ACHLCY_000239"
     CDS             complement(46251..47771)
                     /gene="mglA"
                     /locus_tag="ACHLCY_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP0367569.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDISFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(47832..48830)
                     /gene="mglB"
                     /locus_tag="ACHLCY_000240"
     CDS             complement(47832..48830)
                     /gene="mglB"
                     /locus_tag="ACHLCY_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP0367570.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(49110..50150)
                     /gene="galS"
                     /locus_tag="ACHLCY_000241"
     CDS             complement(49110..50150)
                     /gene="galS"
                     /locus_tag="ACHLCY_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP0367571.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIAPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(50292..51449)
                     /gene="yeiB"
                     /locus_tag="ACHLCY_000242"
     CDS             complement(50292..51449)
                     /gene="yeiB"
                     /locus_tag="ACHLCY_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP0367572.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIAQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(51466..52134)
                     /gene="folE"
                     /locus_tag="ACHLCY_000243"
     CDS             complement(51466..52134)
                     /gene="folE"
                     /locus_tag="ACHLCY_000243"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP0367573.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            52392..53228
                     /gene="yeiG"
                     /locus_tag="ACHLCY_000244"
     CDS             52392..53228
                     /gene="yeiG"
                     /locus_tag="ACHLCY_000244"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0367574.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTIKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(53260..55251)
                     /gene="cirA"
                     /locus_tag="ACHLCY_000245"
     CDS             complement(53260..55251)
                     /gene="cirA"
                     /locus_tag="ACHLCY_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP0367575.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(55544..57013)
                     /gene="lysP"
                     /locus_tag="ACHLCY_000246"
     CDS             complement(55544..57013)
                     /gene="lysP"
                     /locus_tag="ACHLCY_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP0367576.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(57218..58099)
                     /gene="yieE"
                     /locus_tag="ACHLCY_000247"
     CDS             complement(57218..58099)
                     /gene="yieE"
                     /locus_tag="ACHLCY_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP0367577.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            58198..59247
                     /gene="yeiH"
                     /locus_tag="ACHLCY_000248"
     CDS             58198..59247
                     /gene="yeiH"
                     /locus_tag="ACHLCY_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP0367578.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            59321..60178
                     /gene="nfo"
                     /locus_tag="ACHLCY_000249"
     CDS             59321..60178
                     /gene="nfo"
                     /locus_tag="ACHLCY_000249"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP0367579.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            60182..61256
                     /locus_tag="ACHLCY_000250"
                     /pseudo
     CDS             60182..61256
                     /locus_tag="ACHLCY_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(61312..62562)
                     /gene="nupX"
                     /locus_tag="ACHLCY_000251"
                     /pseudo
     CDS             complement(61312..62562)
                     /gene="nupX"
                     /locus_tag="ACHLCY_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(62662..63603)
                     /gene="rihB"
                     /locus_tag="ACHLCY_000252"
     CDS             complement(62662..63603)
                     /gene="rihB"
                     /locus_tag="ACHLCY_000252"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP0367580.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            63733..64431
                     /gene="yeiL"
                     /locus_tag="ACHLCY_000253"
     CDS             63733..64431
                     /gene="yeiL"
                     /locus_tag="ACHLCY_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP0367581.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(64499..65749)
                     /locus_tag="ACHLCY_000254"
     CDS             complement(64499..65749)
                     /locus_tag="ACHLCY_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0367582.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(65843..66781)
                     /gene="psuG"
                     /locus_tag="ACHLCY_000255"
     CDS             complement(65843..66781)
                     /gene="psuG"
                     /locus_tag="ACHLCY_000255"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP0367583.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSATEIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(66769..67710)
                     /gene="psuK"
                     /locus_tag="ACHLCY_000256"
     CDS             complement(66769..67710)
                     /gene="psuK"
                     /locus_tag="ACHLCY_000256"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP0367584.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            67895..68041
                     /locus_tag="ACHLCY_000257"
     CDS             67895..68041
                     /locus_tag="ACHLCY_000257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367585.1"
                     /translation="MPELQKRGICDGMHRTGTLKKGRKESALEEITLVFDERFRFLLK
                     DKTI"
     gene            complement(68134..69825)
                     /gene="fruA"
                     /locus_tag="ACHLCY_000258"
     CDS             complement(68134..69825)
                     /gene="fruA"
                     /locus_tag="ACHLCY_000258"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP0367586.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(69842..70780)
                     /gene="fruK"
                     /locus_tag="ACHLCY_000259"
     CDS             complement(69842..70780)
                     /gene="fruK"
                     /locus_tag="ACHLCY_000259"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP0367587.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(70780..71910)
                     /gene="fruB"
                     /locus_tag="ACHLCY_000260"
     CDS             complement(70780..71910)
                     /gene="fruB"
                     /locus_tag="ACHLCY_000260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP0367588.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQLAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIA
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            72278..73459
                     /gene="setB"
                     /locus_tag="ACHLCY_000261"
     CDS             72278..73459
                     /gene="setB"
                     /locus_tag="ACHLCY_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP0367589.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTN
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(73456..73710)
                     /gene="yeiW"
                     /locus_tag="ACHLCY_000262"
     CDS             complement(73456..73710)
                     /gene="yeiW"
                     /locus_tag="ACHLCY_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0367590.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            73865..74437
                     /gene="yeiP"
                     /locus_tag="ACHLCY_000263"
     CDS             73865..74437
                     /gene="yeiP"
                     /locus_tag="ACHLCY_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP0367591.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            74660..76126
                     /gene="yeiQ"
                     /locus_tag="ACHLCY_000264"
     CDS             74660..76126
                     /gene="yeiQ"
                     /locus_tag="ACHLCY_000264"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0367592.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLVEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     DVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHQGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            76244..77230
                     /gene="yeiR"
                     /locus_tag="ACHLCY_000265"
     CDS             76244..77230
                     /gene="yeiR"
                     /locus_tag="ACHLCY_000265"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP0367593.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            77269..77982
                     /gene="lpxT"
                     /locus_tag="ACHLCY_000266"
     CDS             77269..77982
                     /gene="lpxT"
                     /locus_tag="ACHLCY_000266"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP0367594.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            78394..78960
                     /gene="mepS"
                     /locus_tag="ACHLCY_000267"
     CDS             78394..78960
                     /gene="mepS"
                     /locus_tag="ACHLCY_000267"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP0367595.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            79141..80697
                     /gene="pdeN"
                     /locus_tag="ACHLCY_000268"
     CDS             79141..80697
                     /gene="pdeN"
                     /locus_tag="ACHLCY_000268"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0367596.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            80779..82593
                     /gene="yejA"
                     /locus_tag="ACHLCY_000269"
     CDS             80779..82593
                     /gene="yejA"
                     /locus_tag="ACHLCY_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP0367597.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAASLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            82594..83688
                     /gene="yejB"
                     /locus_tag="ACHLCY_000270"
     CDS             82594..83688
                     /gene="yejB"
                     /locus_tag="ACHLCY_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP0367598.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            83688..84713
                     /gene="yejE"
                     /locus_tag="ACHLCY_000271"
     CDS             83688..84713
                     /gene="yejE"
                     /locus_tag="ACHLCY_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0367599.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            84715..86304
                     /gene="yejF"
                     /locus_tag="ACHLCY_000272"
     CDS             84715..86304
                     /gene="yejF"
                     /locus_tag="ACHLCY_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP0367600.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKSSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(86308..86652)
                     /gene="yejG"
                     /locus_tag="ACHLCY_000273"
     CDS             complement(86308..86652)
                     /gene="yejG"
                     /locus_tag="ACHLCY_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP0367601.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(86985..88175)
                     /gene="bcr"
                     /locus_tag="ACHLCY_000274"
     CDS             complement(86985..88175)
                     /gene="bcr"
                     /locus_tag="ACHLCY_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP0367602.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            complement(88203..88898)
                     /gene="rsuA"
                     /locus_tag="ACHLCY_000275"
     CDS             complement(88203..88898)
                     /gene="rsuA"
                     /locus_tag="ACHLCY_000275"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP0367603.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            89047..90807
                     /gene="radD"
                     /locus_tag="ACHLCY_000276"
     CDS             89047..90807
                     /gene="radD"
                     /locus_tag="ACHLCY_000276"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0367604.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPSIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            90932..91216
                     /gene="rplY"
                     /locus_tag="ACHLCY_000277"
     CDS             90932..91216
                     /gene="rplY"
                     /locus_tag="ACHLCY_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP0367605.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(91355..92362)
                     /gene="yejK"
                     /locus_tag="ACHLCY_000278"
     CDS             complement(91355..92362)
                     /gene="yejK"
                     /locus_tag="ACHLCY_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP0367606.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGMPPNLRDQLQRRTSGG
                     N"
     gene            92544..92771
                     /gene="yejL"
                     /locus_tag="ACHLCY_000279"
     CDS             92544..92771
                     /gene="yejL"
                     /locus_tag="ACHLCY_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP0367607.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            92791..94551
                     /gene="yejM"
                     /locus_tag="ACHLCY_000280"
     CDS             92791..94551
                     /gene="yejM"
                     /locus_tag="ACHLCY_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP0367608.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            94626..94702
                     /locus_tag="ACHLCY_000281"
     tRNA            94626..94702
                     /locus_tag="ACHLCY_000281"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:94660..94662,aa:Pro,seq:ggg)
     gene            94921..96162
                     /locus_tag="ACHLCY_000282"
     CDS             94921..96162
                     /locus_tag="ACHLCY_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0367609.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLIKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALIRAGTDPQDHERQKRETEARR
                     QGNTFENVATAWYQVKISQNLAPNTIKDIWRSLEKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGMIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPVEASSARWEEIDMEAQRWTIPAGRMK
                     MRRDHVIPLCDQAMAVLEAMKPISHHRDYVFPSLKDPRQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRVVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            96659..96865
                     /locus_tag="ACHLCY_000283"
     CDS             96659..96865
                     /locus_tag="ACHLCY_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0367610.1"
                     /translation="MEAIKKVIFRQEVKKLIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEEDVIAWMKEREQEARGTTA"
     gene            complement(96987..97190)
                     /locus_tag="ACHLCY_000284"
     CDS             complement(96987..97190)
                     /locus_tag="ACHLCY_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367611.1"
                     /translation="MTNKESTNTPSQKTNREHENMALEHESERFAPCAFVLDEFLKKY
                     SRSERMKMAAQYGPNKQDNCPPA"
     gene            complement(97248..97421)
                     /locus_tag="ACHLCY_000285"
     CDS             complement(97248..97421)
                     /locus_tag="ACHLCY_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367612.1"
                     /translation="MSTPLEKRSPPYQMRLPEEFRAQLEEAAKADGDTSLATWIKRVL
                     RKELQQRGIEPKG"
     gene            complement(97926..98231)
                     /locus_tag="ACHLCY_000286"
     CDS             complement(97926..98231)
                     /locus_tag="ACHLCY_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0367613.1"
                     /translation="MATGSKNAKSQSLTARIPHDVIEGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENLLISSLNALAQVEKLGIKAAEEIGQLVTVAREELQRRKAKESE"
BASE COUNT        24354 a        25757 c        24475 g        23924 t
ORIGIN      
        1 tgtaaatggt attgttgata tcaataaaaa agccgaaatc atttatataa atgatttcgg
       61 ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg
      121 acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga cgggcgaaga
      181 aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt gaggttgacc
      241 attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg ctaatgtggc
      301 agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa ttcctgacag
      361 ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt
      421 gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt
      481 gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt
      541 attaaataag tcttcattaa tgcggcaaaa actaatacac cgcatcaatg taacatctct
      601 gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct
      661 cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc
      721 ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga
      781 gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgtg tggtattttt
      841 cagccagttt ctcagcacca ccggtaccga aaatcggttc atggtgacca cagttactgc
      901 aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact ttctcgaaca
      961 tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg
     1021 caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca
     1081 tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc tgcattaacg
     1141 ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc agataaccaa
     1201 ttgaattggt tgccaggcca tgagacataa tcggtgccat gtgggtaccg tccggcgacg
     1261 ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt
     1321 ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg
     1381 aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acaccattaa
     1441 tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga
     1501 tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa
     1561 acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg
     1621 cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat
     1681 tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt
     1741 cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa
     1801 tatagagaag tacttactta acattttccc atttggtact atctaacccc ttttcactat
     1861 taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg
     1921 ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc
     1981 tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc
     2041 cacggtacac cgatcatgct gaaagctcag cagctcggta tcaccccgga gcagatgatt
     2101 ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac
     2161 tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg
     2221 aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc
     2281 atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa
     2341 tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt gatcgagccg
     2401 aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat
     2461 ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag
     2521 gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga tatctcccgc
     2581 gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg
     2641 ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac
     2701 agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc
     2761 ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga aggcagcaac
     2821 ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg
     2881 tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac
     2941 agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac
     3001 ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt taacctggcc
     3061 tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaactggct
     3121 gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa
     3181 agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc
     3241 tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgccgacctg
     3301 caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta cctgaagccg
     3361 gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact gacctgggat
     3421 ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct gtataaccgc
     3481 atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct
     3541 gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac
     3601 gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt tgttgaaggt
     3661 tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc
     3721 ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac cattatggtg
     3781 gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt aatggctgct
     3841 ggtcctggcg ggaaagatat tttcctgcta agcccggatg ccggtgctaa accgggtcat
     3901 caggtgaaat aatccccctt caaggcgctg catcgacagc gccttttctt tataaattcc
     3961 taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatcag
     4021 cattcaactg gataaaatta cagggatgca gaatgaaaca ctttatctat caggacgaaa
     4081 aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc agttggggaa
     4141 aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg gcagcggcaa
     4201 aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag caagcgaagg
     4261 ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg gacttatcca
     4321 ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac aaaaatacag
     4381 atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac
     4441 acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga
     4501 cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca gccacatttg
     4561 acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg
     4621 acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg gcacttgaaa
     4681 tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg
     4741 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt
     4801 atgcgaataa tgtcgttatt attctgccgt ctggcattgc acctgactac ttggagcaat
     4861 attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc
     4921 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatgg cggcaaccat
     4981 taattgcttt gttattacct gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     5041 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     5101 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
     5161 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     5221 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     5281 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cggatgacga
     5341 aagccattgc tgcgttccca catgcagcaa tggcggcact gacggaactt cttgggcaaa
     5401 aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctaacaa ccagcactgg
     5461 cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc
     5521 aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc gtagtagtct
     5581 cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5641 taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5701 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5761 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5821 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5881 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag
     5941 taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac gccatgttac
     6001 attttggact caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag
     6061 ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct
     6121 tcaacaaact gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac
     6181 atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca caggataacg
     6241 cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg caagaaatcg
     6301 cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc
     6361 ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct
     6421 ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc ctcctccacc
     6481 tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta ttgcaggtga
     6541 aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt accgcctggc
     6601 agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc gttctcggta
     6661 acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctagt gaatcccagc
     6721 ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctta
     6781 tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag
     6841 aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt gaagatcggt
     6901 tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt ggcccacggc
     6961 agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc
     7021 gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga atcgcggtcg aacgatgcgg
     7081 ttaaccgcta caaattgctg aaaaaagatg cgcgtaccgt cgccgcccag caggtagcaa
     7141 ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgcc ggaaaacttc cagctcttcc
     7201 tggttgagca tccgctggtt cgccacttaa cccgccgtct gatttggggc gtttatagcg
     7261 ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac agcaccgctg
     7321 acgatgatct tttcaccctg ccggaaggcg atatctctat cggcattcct cacgttctgg
     7381 agatatcacc gacggatgcc gccgcctttg ggcagctttt tgccgactat gaactgttac
     7441 cgccgttccg ccagctcgac cgtaacagtt acgccctgac agaaaccgag cgcaatgcca
     7501 gcgaattgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg ggactggcga
     7561 ataaaggttg ggtgcgtggt gaaccgcagg atggtggctg ggtcggctgg atgatcaaac
     7621 ctctgggccg ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt ggtatgtcgc
     7681 cagccgaact tagcgccgag caactcttaa gcaagctgtg gctatgggaa ggcaaagcag
     7741 aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca
     7801 tcaccgccag cgagctaatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     7861 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     7921 aaacgccgct cagtcatcgt cgctggccgg gcgaaccagt gtccgttatc actggaagtc
     7981 tcatccagac attgggagat gaattactac aacaagcagg ccagaaggaa aatatcacct
     8041 ggaattatga taaatgctca ctggagtggc agtccgccat ccagcaggca atcaatttga
     8101 ctggcgagca taaaccgtca atcccagccc ttaccatggc ggctctgatc tgtatcgcgc
     8161 aaaatgacag ccagcagtta cttgatgaaa tcgtgcaaca agaggggctg gaatatgcga
     8221 cggatgtagt gatagcgcgt cagtgcattg cgcgacgtta tgagagtgac tcactggtcg
     8281 tcactttgca gtatcaggac gaggactatg gctacggtta tggctccgcg acctataacg
     8341 atttcgatct ccgtttgcgt aagcatctgt cgctggcaga ggaaagttgc tggcagcgtt
     8401 gtgccgataa actcattgcc gcactgccag gaatacccaa aattcgccgt ccttttattg
     8461 cgctgatcct cccggaaaaa cctgaaatag ccaatgaact cgccagtctt gaaagctctc
     8521 ggtcaagtct ccattcaaag gagtggttaa aggtcgttgc tactgataat acggcggtaa
     8581 aaaaactcga acgatactgg ggcctggacg tatttagcga tcgcgaagcc agctacatgt
     8641 cacaagaaaa ccgcttcggc tacgcggcct gcgcctccct tttgcgcgaa caaggactgg
     8701 cagccgttcc gcgcctggca atgtatgccc ataaagaaga ttgcggcagt ctgctggtac
     8761 aaattaacca tccgcaagtc atccgcacct tgctactggt ggctgataaa aacaaaccca
     8821 gcctgcaacg tgtggctaaa tacagtaaaa acttccccca cgcgacgctc gccgcactgg
     8881 cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc atcgaagaca
     8941 aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc
     9001 tgatggcatc gcagccacaa ctggcagcag aagtgatgcc gtggttaagt actcaagcca
     9061 gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat agcaccgata
     9121 acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag
     9181 ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg caaccaggcg
     9241 aacaagaggg gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgg
     9301 aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg
     9361 tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca
     9421 ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc
     9481 cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca
     9541 tatgggaaaa tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt
     9601 tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc attgagaaaa
     9661 agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag
     9721 gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg
     9781 gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg
     9841 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc
     9901 ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt
     9961 acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg
    10021 tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca
    10081 ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta
    10141 ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
    10201 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
    10261 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
    10321 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga
    10381 cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga
    10441 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    10501 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggta gtcgcagaaa
    10561 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    10621 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    10681 acctcaaccc ggttatcttt aatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc
    10741 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    10801 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
    10861 cccgacgctg gtcgctggca gattttcatt ctctgtttgt taatcatccc tttacccgtc
    10921 tggttaccca gcgattaata tggggggttt atccggcaaa tgaaccgcgt tgtttactca
    10981 aagcctttcg tgtggccgca gagggggagt tctgcaatgc gcaagatgag ccaattggcc
    11041 tgcctgcgga cgctctgatt ggcattgccc acccgttaga aatgacagta gaaatgcgca
    11101 gtgaatttgc acagcttttt gccgattacg aaattatgcc gccttttcgc cagttgtcgc
    11161 gccgcacggt gctgctcaca cctgacgagt caaccagtaa cagcctgact cgctgggaag
    11221 gtaaatccgc taccgttggg cagcttatgg gaatgcgata caaaggctgg gagtcaggtt
    11281 atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg
    11341 gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgc
    11401 accgtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt
    11461 taagcgcacc tgacgtcatt ttccattaaa aacacagcgg gcagtgatgc aactgcccgt
    11521 tatcaacacg acttaccagc ggggaaagcg atgatcgtgc aaaaagagct ggttgctatt
    11581 tacgattatg agatccctgt acctgaaaat ccgttttcct tcagacttga gatccataaa
    11641 tgctctgaat tatttacagg ttccgtctat cgactggagc gattccggct acgtccaaca
    11701 tttcatcaac gtgatcgaga agatactgac ccgctaataa atgatgcgtt gatttatata
    11761 agagatgagt gtattgatga gcggaaatta cgaggtgaat cacctgaaac tgtaatagca
    11821 atttttaatc gtgaactaca gaatatattc aaccaagaaa tagaataata tactctagat
    11881 aattaaagtc gcagcacatg caacttgaag tatgacgagt atagctttct taaaggggga
    11941 aacggtattc ccctttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc
    12001 gtaacagagc cactaataac tccagtacgg caatgagcac ggttaacttt tgtgtcagcg
    12061 tgtccatccg ctctccttat taaggagcgc aacaccagca ccttaccctt acactgcctg
    12121 ttgatactgg tttacgtatg ttggtacgaa acgttgctct ccggatcagg gcaccgcctg
    12181 cggcaccccc cgtccggcag ccaggactta aaattggtcc gatccggcac aacctcccaa
    12241 cgggcaggat tataaatctc actcactgaa ttgcaatgga gtgtggatat ggttataatt
    12301 tattcgttgg tacgaaatgt tgcacaatca gcaagttagc gtcactgtag taaataaaag
    12361 aacctgcctc accagcaggt ttttttattt tctgcgatct actttccaga tattttttgc
    12421 caatagccca acataaatat tcacatcccg gtcaaatggt gcgttgaata taaaatcgat
    12481 gaatttataa ataaaaatga gttatatgat gaattaataa aaacagtaat cattttatta
    12541 aagggggaag cggttttccc ccttagatgc gtttcaattt gcccgttact tcaacaaatc
    12601 aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg ttaacttttg
    12661 tgtcagcgag tccatccgct ctccttatta aggagcgcaa caccagcacc ttacccttac
    12721 actgcctgtc gatactggtt tacgtatgtt ggaccacgat gttgcactcc ggatcagggc
    12781 accacctgca gcaccccccg tccggtagcc aggacttgaa ataggttcga tccggcgcaa
    12841 cgtcccaaca gtcaggatta taaatctcat tatcttaatt gcaacggggt ccagccgtgg
    12901 ttataattcg ttcgttggaa cacgatgttg cacaggctgt ggtgttaggc ctgaaaatag
    12961 taaataaaag aacctgcctc accagcagtt ttttttattt actgtaatct gctttccaga
    13021 tattttccgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat atcgtttatt
    13081 tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct gcagcgtccg
    13141 cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga taacgcacct
    13201 tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct tggcgatagc
    13261 gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg tatgttagtc
    13321 actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc aaaatctctc
    13381 ctgtctgaat tactggcaac cgctattagc ggcgatgccg ggttaaccat ccagggcggc
    13441 gcatctacca ctgaagatca aatcaagtac ggctggaact acgctctgct tatcaatcat
    13501 ggcccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg cgacggcaaa
    13561 atcgtccgtt tcgaagagat aacgcgtaca ccgctggaag tgcaagattg tctgttaggt
    13621 atgctttctg acagagttat gacggtgccg gaactcactg gtgaagccag tcaactctat
    13681 gcgcgggaag gctttaatat tattgccact gccaataccc gcgatcgcgg cgtcaatgag
    13741 atgagtgccg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat tatggatttc
    13801 gctcaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca tagcggtatt
    13861 ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg cgatctgcgt
    13921 gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc caccgctgaa
    13981 gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa tcgcgcaggt
    14041 gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa cgaagaagat
    14101 cgcgctcgtc tgcgccgtta cttcgaacaa cgagtcgcca cacataaaga agctcactgg
    14161 caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga gcgagccgtt
    14221 aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat cgttaatcga
    14281 aagccagcgg ccacgatacg tgttgattga aggcccggct gattttaatg accgggtaga
    14341 cgaactgttt ttatcccacc agcttccggt agctatttac agttattgcc agtatcagga
    14401 cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc cggagtggca
    14461 ggcgctacaa gccgcacgtc gtattcaggc acaaacttac ttcatcgatt tgccttgctg
    14521 ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gatgaaagcc aggccttact
    14581 gctgcgtgcc acccgcatgg ataacagcga taccctgtgg gatcacttgt tcgaagatga
    14641 aagccagcaa actgcattac cctctgcgct ggcgcactat tttgcccaac tgcggggcga
    14701 tttccccggc gatgcactca atcgtcagcg cgaagccttt atggctcgct ggattgcatg
    14761 ggcggtgcag caaaataatg gcgacgtgtt agtcgtctgc ggtggctggc acgctccggc
    14821 actggcaaaa atgtggcgcg aatgcccgca ggacattaac acgccggaat tgccctcgct
    14881 ggcagatgcc attacaggtt gttatctcac gccctacagt gaaaagcgcc ttgatgtgct
    14941 ggcaggatac ctttccggta tgcctgcccc ggtctggcaa aactggtgct ggcagtgggg
    15001 cttacagcag gccggtgaac aactgctaaa aactatcctt acccgtttgc gccagcacaa
    15061 gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg cgctggcaca
    15121 gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag caggctcgct
    15181 gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta ttcatcccga
    15241 taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat tcggtaaact
    15301 tgccccttct acgccacaac cgcctctgcc aaaagatgtc acctgcgaac tggaacgtac
    15361 cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg gactagcgca
    15421 aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac gccagcaggg
    15481 aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc gcccgcttag
    15541 ccagcatgcg gcattgattg aggccgcctg ctttggtgca acactccagg aagccgcacg
    15601 ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagtatca ccacatgtct
    15661 tagccaggcg gcgttagcgg gtctggcgtc cttcagtcaa caattactgg agcaactcac
    15721 attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg aagtgctata
    15781 tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat tacaaacgac
    15841 gttatgcgcg gctatcgatc gcacgctgtg gctatgtgaa tctaacggca gaccagatga
    15901 aaaggagttt cacgctcacc tgcatagctg gcaagcgttg tgtcatatcc tgcgcgatct
    15961 acatagcggt gtgcaactcc ccggcatttc tctttctgcg gcggtagcct tactggagcg
    16021 acgcagtcag gcaattcatg ccccggcgct ggatcgcggc gcggctcatg gcgcactgat
    16081 gcgtctggaa catcctaacg ccagtgccga agcggcgctg acgatgctgg cgcagttatc
    16141 cccggcacaa tccggcgaag cgctgcacgg tttgctggcg ctggcccgcc atcaactggc
    16201 ctgtcagccg acatttatcg ccggtttcag cagtcattta aatcaactaa gtgatgccga
    16261 ttttatcaat gccctgcccg atttacgcgc ggcgatggcc tggctaccac cacgagaacg
    16321 cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc ccgtttcggc
    16381 gctgcaaatg ccgttgcatt gtccaccaca ggccattgca catcatcaac aactcgaaca
    16441 gcaggcactg gcatcgctgc aaaactgggg agttttccat gtctgaactg aacgatcttc
    16501 tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca gaaacgacgc
    16561 tttgtgggct ggatgacaac gcccggcaga tagaccacgc gctggagtgg ctgtatgggc
    16621 gcgatcctga acggctccag cgtggtgaac gctctggtgg attaggtggc tcaaatctca
    16681 ccacccctga gtggatcaac agtattcaca ctctgtttcc gcaacaggtg attgagcgac
    16741 tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacgaat ctcgacgtgc
    16801 tggaacgtat gcagccttcg gaaagcctgc tacgcgccgt tttgcacacc aaacatctga
    16861 tgaatcccga agtactggct gccgcccgcc agatagtgcg ccaggttgtt gaagaaatta
    16921 tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat cgccgtcgcc
    16981 gctcattaat tccactggcg cgaaactttg atttcaaaag tactctgcgc gccaacctgc
    17041 aacactggca cccgcaacac ggcaagttgt atatcgaatc cccccgcttt aacagccgca
    17101 ttaaacgcca aagcgaacaa tggcaactgg tcttactggt tgatcaaagc ggatcgatgg
    17161 tggactcggt gatccactct gcggtgatgg cggcctgttt gtggcagtta cccggcattc
    17221 gtacccatct ggtggcgttt gacacaagcg tggttgatct cacggcagac gttgccgatc
    17281 cggtagagtt attaatgaaa gtacagttgg gcggcgggac caatatcgcc agtgccgtgg
    17341 agtatggtcg gcaacttatt gaacaaccag caaaaagcgt cattatcctc gtgagcgatt
    17401 tttacgaagg gggttcatca tcattgctga cgcatcaggt gaaaaagtgt gtccagagcg
    17461 gcatcaaagt gctgggactg gcagcgctcg atagcaccgc aacgccttgc tatgaccgcg
    17521 atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgacg ccgggcgagc
    17581 tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg aattattaga
    17641 actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc gcagtcttaa
    17701 ggaactggaa aatggcaacg tcccggaaat cagccatgag aacggcgctt taatcgccac
    17761 cttcagtgac ggtgtccgta cccagctggc gaacagccag gcactgaaag aggctcagtg
    17821 cagctgcggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa gttatcaacg
    17881 actttgtgcc accgtccagc ccacggaaaa agaagaagag tgggatccgg caatctggct
    17941 ggaagaactg gctaccctgc ccgatgccac ccgcaaacgc gcgcaggcgc tggtcgctaa
    18001 aggcatcacc attgagttgt tctgtgcgcc gggcgaaatt ccctctgccc gcttaccgat
    18061 gagcgatgtg cgtttttatt cccgtagcag tattcgtttc gcccgttgtg attgtattga
    18121 aggcacactt tgcgaacatg tcgtactggc tgtgcaggcc ttcgtcgagg ccaaagcgca
    18181 gcaagcagaa tttaaccatt taatctggca gatgcgcagc gaacacgtca catcatctga
    18241 cgatccgttt gccagcgaag aaggccaaac gtgtcgtcaa tatgttcagc aattaagcca
    18301 ggcattatgg cttggcggca tcagccagcc gcttattcac tacgaggcag cattcaaccg
    18361 cgcattgcag gcggcagagg cctgcaactg gcgctgggtg agtgaatcac tacgacagct
    18421 acgggcaagc gttgatgcct tccacactcg cgccagccac tatcatgccg gagaatgctt
    18481 acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg cccggcgcga
    18541 cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg gcattgccgg
    18601 agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat
    18661 tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta ttttgcacct
    18721 ttcgcgcagt tggccgcgaa gtgaacagga aaactcaccg gcagctacgc gtcggctgtt
    18781 tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag caaaacgcag
    18841 tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg tgccgctgtc
    18901 gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg tggctttgcg
    18961 ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg tcgataactt
    19021 atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc gccagacgct
    19081 ggatgcgcag gtaatcagcg gcgaaggtga agataatctc ctgacgttat cccttccggc
    19141 atctgcctgt tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga
    19201 ccccgtgagt ctggtttctg gctttgtcag ttttgttgat gggcaattga cactggaacc
    19261 acgggtgatg atgacaaaaa cccgcgcctg ggcgctggac gcggaaactg cgcctgtggc
    19321 acctaccttc tgccagcgtt ttgcctgtgc cgtctaccgc tcatcagttg ctgatgcgct
    19381 gccaggcgtt acttattcaa ctgctccata acggctggcg ctatcaggaa cagagcgcta
    19441 ttagtcaggc agagttgctg gcgaatgacc tctccgcggt cggtttttat cggctggcac
    19501 atgtgttggg acaatttcgt aatacagaaa gcgaggcacg ggtagaagca atgaataacg
    19561 gtgttttact ttgcgaacaa ttattcccca tgcttcagca acaaggatga aatagtgctt
    19621 tttactaaga gttctactcc agttccggac tgctcacgtc acggtattag gcatattcta
    19681 tatagcccct ggtgagagtc accagtttct tgattaaata aaatggagtt ttacatgaag
    19741 gctttcaata agctgttttc cctcgttgtt gcatctgttc tggttttctc tcttgctggc
    19801 tgcggtgaca aagaagaatc gaagaaattc agcgccaatc tgaacggcac tgaaattgcc
    19861 attacctatg tctacaaagg tgacaaggtg cttaagcaat cttctgaaac caaaattcaa
    19921 tttgcttcta ttggtacaac caccaaagaa gacgctgcca agacacttga gccgttaagc
    19981 gccaaataca aaaacatcgc gggtgttgaa gaaaaattaa cctatacaga tacctacgcg
    20041 caggaaaacg tgactatcga tatggaaaaa gtggatttta aagccctgca gggtatttca
    20101 ggaatcaacg tttctgctga agatgccaaa aaaggtatca ctatggcgca aatggaactg
    20161 gtgatgaaag ccgctggttt taaagaagtg aaataatcgg ttggcggtca tgctctaaac
    20221 atgaccgcca attttttagc cttttttcac atgctggcgc gctgccagtc cacgcagaaa
    20281 ataacgtaaa aattgatcgc cgcattcgcg gaagttttta tgatccggtg cgcgcatcat
    20341 cgctgtaatt tccggcatcg aaacgcggaa ctgctgttcg gtgaggatag ccagaatgtc
    20401 atcggttttc agcgaaaacg cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg
    20461 acgttccggc tccagtgccg gagcagactc atccttgccg cgtttttcat aaatcaggcc
    20521 attgaggaat gacgacaaaa aaatgtccgg acaacgctga aaaccctctt cgtcttcttt
    20581 acgtagccag acggcgatct gttccgcggt ggcttcgaca ttacccagcg ccagaatacg
    20641 caccaggtca ttattattgg ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact
    20701 tagcatgagg ccttcaggtg ttgatgaggc aaaaagccat tttagcagtc ttttacaggc
    20761 caatcgcctc ttttaagctt ttcagataac ggcggctgac cggcacggtt aagccattac
    20821 gcaaaatcaa ctcggcctgg ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt
    20881 taaccagata ctgacgatgg cagcgcagta gtggtgtacg actttccagg gtacgtaatg
    20941 tcaattcggt aaagccctct ttcccttcgt ggctggtaac gtagacaccg ctcatccgac
    21001 tgctgacaaa tgccacatct ttcatttgca gcaaataaat ccgactatgc cccgtacaag
    21061 ggataaattt cagcgcctgt tgattttccg gtaacagcga aacatcctgc ttgctgcgct
    21121 cctgacgcaa tcgcgccagc gttttctcca gtcgcgcttc atcaattggc ttcagcagat
    21181 aatcaaaggc atgttcttca aaggctttaa tagcgtattc gtcaaacgca gtgagaaaaa
    21241 caatatacgg gcgatgttcc gggtcaagca tccccaccat ttccagacca ctgatgcgcg
    21301 gcatctggat atcgagaaac agcacatccg ggcgcagttt atgcaccgcg ccgatccctt
    21361 ccacggcgtt tgaacactct ccaacgattt caatatcgct ctgctcctgc aaaaatacgc
    21421 gcaggttctc ccgtgctaac ggttcatcat cgacaattaa gactttaatc atgcctcgtc
    21481 cctccatggt agtcgtaacg ttattcgggt gtaactatca ggctcacagg cgacgcttat
    21541 tccatagtca tcgccaaacc gttcacgtaa acgcttatcc accagattca tccccagccc
    21601 actggcattg gttaccggtt gatacaaacc ggcattgtct tcgatctcca gcatcaaatg
    21661 ttgcccctca cgtcgggcgc tgattgccac tcgccctgta tccagcagtt gtgatgtccc
    21721 atgtttaatg gcgttttcca ctatcggttg cagggtaaac gcgggcaatt gctgctggga
    21781 taattcttgc ggaatagcaa tgttgacctg caaccgcgac tggaagcgcg ccttttcaat
    21841 ttgcagataa gcgttcacat gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg
    21901 ctttaagttt ttgcggaaaa aagtggaaag atactgcacc agctggctgg cctgttcgct
    21961 gtcgcggcgg atcaccgctt taatggtgtt aagcgcatta aacaaaaaat gaggattcac
    22021 ctgggcgtga agcagtttga tttctgactg ggtgagcatc gctttttgcc gctcatattg
    22081 cccggcaaga atctgcgccg aaagcagttg cgcaatcccc tcgcccagcg tgcggtttat
    22141 tgaactgaat aaacggtttt tggcttcata caatttgatg gtgcccatca cgcgctgatt
    22201 ttcgccacgc aacggaatta ccagcgtcga ccccagtttg cattgcggat gcaaagagca
    22261 acgataaggt acttcgttgc catcagcgta gaccacttca ccggtttcaa tcgcttttaa
    22321 ggtgtaagtc gaagaaatcg gtttgccggg taaatggtgg tcgtcaccaa ttccggtaaa
    22381 ggccagcaat ttctctcgat cggtaatcgc gactgcacca atatccagct cctgatacag
    22441 cacctgagcc actttcatgc tgttcacttc gttaaacccc tgtcgcaaaa tgccttccgt
    22501 cgaggctgcc actttcagcg cagtggcaga aaaagcagaa gtgtattttt caaacatcgc
    22561 gcgtttatcg agcaatatac gcataaacag cgccgcgccg acggtattgg tgaccatcat
    22621 tggtgcagca atattactca ccagacgcac cgcatcttca taaggtcggg cgatcgcaag
    22681 gatgatcagc atttgcacca tttcagcgac gaacgtgacg gcaccggcgg taatggggtt
    22741 aaagacttta tcagtgcgcc cgcgacggat caggatgctg tgtaccaggc cgccgagcaa
    22801 tccttcaacg atggttgaga tcatgcaact tagcgcggtc atgcccccca tcgaatatcg
    22861 atgtaagccg ccggtcagac caaccagccc accgacgacc ggaccgccga gtaagccgcc
    22921 cattaccgcg cctatcgcac gggtattggc aatagaatcg tcaatgtgca acccaaacca
    22981 ggtgcccatg atgcagaaga tggaaaagac gatgtagcag agaaatttat gcggcagacg
    23041 aaccgtgacc tgcattaacg gtatgaataa tggcgtttta ctcattaacc acgcaatgac
    23101 taaaaaaacg cacatctgct gaagcagcag caacaccaga ttaaaatcgt acatacccgc
    23161 aaaccacact tccctttaaa acgcgtaaca tacattgcct gcgtttaact ttctttgaac
    23221 tcttgcagaa aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa
    23281 atcaccctat agatgcacaa aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa
    23341 agttaaaccg ggacctcgcg agcaagggtg agacgatggc gctttacaca attggtgaag
    23401 tggcgttgct ttgtgatatt aaccctgtca cgttacgcgc gtggcagagg cgttacgggt
    23461 tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc gatattgacc
    23521 gtatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa gttaaaatgc
    23581 tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa gaaacattac
    23641 tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa gagcgcggtc
    23701 aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc cgacgacttc
    23761 aatgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta ctgatcaact
    23821 acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg ctggtggttg
    23881 gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc agtcaacaag
    23941 gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa ctattcgaag
    24001 gtcgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag cagcaactca
    24061 ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga ttcgttaaca
    24121 aatgcgcttt actgtacaat cctttcgtta acataaggag tgcattatgc gcatagctaa
    24181 aattggggtc atcgccctgt tcctgtttat ggcgttcggc ggaattggtg gcgtcatgct
    24241 cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct ttcaaacagg
    24301 cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac atacgcggta
    24361 ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc taccgttgag
    24421 gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc cagaatcacc
    24481 ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat tcccatacct
    24541 ttcgcaactt ctgtcacgct ggcatcgatc gctcccagcc cggcaagtgt cgcctgcagg
    24601 acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc aaagccgatc
    24661 accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac ggcggcaata
    24721 gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat tccggcacca
    24781 gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg agccagtgcc
    24841 agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa caaggcagca
    24901 aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc aatgagccag
    24961 aacagcggat cgcgcaacat cttcatacgc ttacgcctcc accagcagat cctgaaaatg
    25021 cagcgtaccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc aaccccgcgc
    25081 gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct caccttctgc
    25141 ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcagac gcacacccag
    25201 ttcgctacgt ccaaaaaact ggcggacaaa atcattcgcc ggacgagtca gcatcgtcag
    25261 cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt cagccagccg
    25321 tagcgcctca tcaatatcat gagtgaccag cacaatagtg cgccccagca aacggtgaat
    25381 gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc caaaaggttc
    25441 atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca cacgttgctg
    25501 ctgaccaccg gaaagctgat gcggataacg ctcacgcaaa tttgactcca gccccagtag
    25561 cgtcattaat tcgtcgatac gatcgtcaat ccgtgcccgc gaccattttt gtaattgcgg
    25621 cacggtagcg atgttttgcg ccacgctcca gtgggggaat aggccaatag attgaatggc
    25681 atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt ctccggcaaa
    25741 gcggatctct ccgctgtcat gttccaccag gcggttaatc attttcaagg aggtggattt
    25801 gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat tgagattaag
    25861 atcgttaacg gctttttgtg cgccaaacag tttgctgaca tggctaaatt caatcattac
    25921 gtttcacctt cagcagtgcg ataagcaaat cgaacagggc atcgatcagc accgccagaa
    25981 caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc agcccctgga
    26041 aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc atacctacag
    26101 tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt ggtaactgaa
    26161 catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc agcacatcgc
    26221 gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag gcatagagca
    26281 ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcttc cccagccacg
    26341 gaaaggccgt caccagcgcg gcaagcggcg caatcaacag gccaaagagc gccaccgaag
    26401 gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca gtggaaaagt
    26461 agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca ccaaacagca
    26521 acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg tattctttca
    26581 ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata atggcaatct
    26641 gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg gcatcgctac
    26701 aggccagcaa tgccagcgcc gcagccagcc agaaaccact gccgaggctg gtacgcgcca
    26761 gcgcactgcc attttgcgcc agttgtgtcg ccgcctttcc cgcgccccac accagcaata
    26821 cgaagacgaa ttgcgccaga atgagtgcac aaatgctgcc ttttttaccg ggaataaaac
    26881 aggccgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc ggccacagtt
    26941 gccagagatg acgcccttca cccgagacta aacgattagg cgcgtagctg ataaacggca
    27001 gcgctgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta atacgtagat
    27061 aagtcacggg aaattacttc gcccaccctt tttgtttcag gtagtcggca gccacttttt
    27121 tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt tttgcatcga
    27181 ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactccttc aacaccgatt
    27241 cacgcaccac tggtgcaggc gcatagatag gttgcacgcc ttgcggatcg cttaaggttt
    27301 gcagccccag cgccgctacc gagccgtcag tgccgtaagc cattgcagca ttaacgccag
    27361 aggtttgctg ggcagcggct ttgatcgtca ccgccgtgtc gccaccagcc agtgacagca
    27421 actgatcctg accgagctta aagccatagg ctttttcaaa cgcgggtaac gcatcggcgc
    27481 gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctcttgc agataacgac
    27541 tcaggtcggc aagcgaagtg agtttatttt tctctgccac atcctgacgc acggcgatag
    27601 tccaggtgtt atttgcaggc gcgggcgtca gccagattaa cttgttgtgc tccgaatcga
    27661 gctttttgac tttctcgtaa ccttgctgcg cgtttttcca cgctgcatcg ttttcatctt
    27721 taaagaagaa agcgccattg ccggtatatt ccggatagat atccagttca ccggaagtaa
    27781 tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc ggtacgccgt
    27841 ggctttccag cacctgcaaa atgatattgc cgagtagcgc accttcggta tcgatttttg
    27901 aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc aacataacca
    27961 gtgaacctgc ccagagcttt aagagtggca tgatgctttc ctcattcttt tactgttgtt
    28021 ttcagcgaat taagagaaaa gcatagttga taatggcggg gttagcttga gcggaaggat
    28081 tcagttgcag aatcagataa atacttaaga ggcatattcg gtccggcgtt tcctgccgga
    28141 tgcggcgcga gcgccttatc cggcctacaa agggcgcaaa cgtcgcgccc ttactaaagc
    28201 attacagcaa ctcaaactcg cctttcttaa cgcgtgcgga atcagtgccg ataaatacat
    28261 tgaacttgcc aggctcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcaa
    28321 tatcgatcgg gaagctgaca gtctgagttt cgcccggttt cagggtgatt ttctcaaagc
    28381 ctttcagctg tttcacaggg cgactcatgg aagccgtcac atcctgcaag tacatctgca
    28441 ctaccgtggc cccctcgcgc ttaccggtgt tcgtcacctg cacgctggca gtcactttgc
    28501 cgtcacgctt catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtgtagc
    28561 tcagcccata gccgaacgga tacaacgccc cgttagcttc atcaaaataa cgcgaagtgt
    28621 atttgttcgg cttgtcggca ttatacgggc gaccggtatt cagatggctg tagtacaccg
    28681 ggatctgccc gacagaacgc gggaaggaca ttggcagctt gccggacggg ttgtaatcgc
    28741 caaacaatac atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa
    28801 tcgcatcagc ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca
    28861 gcaccagcgg tttaccggtg gctttcagcg ccgcaatcaa gtcacgttgg ctttgcggaa
    28921 tagtgatatc ggtccggctg gaggcttcgt gcgccatccc ctgtgcttca ccgactacag
    28981 ccaccaccac atcagattgt ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg
    29041 aacgcggatc gactttgacc gcttcttcat actgattcag gaaatcgata atgcctttgt
    29101 cactggtaac gttcgcccct ttggcataca gcactttacc gttttcaccg acggaatttt
    29161 taatcccggt cagtacggtc acggattgat cggcaacacc ggctgcggac cagctgccca
    29221 tcacgtcacg tttactgtcc gccagtggcc caaccaccgc aatggtggcc gattttttca
    29281 gcggtaacgt ttcgagacgg tttttcagca acaccaggct ttcgcgcgcc acttcacgcg
    29341 cttctttacg gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc
    29401 ccaaatggct gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc
    29461 gggcagcatc gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat
    29521 acttcgagta atactcgtcg ctcatactca tgttgattcc ggatttcagc gccacgcgca
    29581 ccgcatcttc cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcaccgtgat
    29641 cggaaacggt gatgccttta aagccccact ggtcgcgcag aacatctttc agcagccagg
    29701 aatcggaggt ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcgccgctgc
    29761 ctgcgtccag ccccgctttg tacggcggca tataatcatt aaacaggcgc tgcggactca
    29821 tatcgacggt gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga
    29881 cgctggtcat caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg
    29941 ttttacccat tgttgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc
    30001 agcgcggatc gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg
    30061 ccgcttcata agcagagaca cgtccgaccg ttttcactgc atcgaggtta aaagacgagg
    30121 ccagaccgag gctaatcggg aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga
    30181 aaagaggaat tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct
    30241 gacgggttac ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg
    30301 cctctttcgg gttatccggg ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa
    30361 ctgtcatttt cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat
    30421 ggttgccgaa taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta
    30481 ctgaacatag ccatttcata tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa
    30541 tggtgcaaaa accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt
    30601 attgtctgaa ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt
    30661 tgtgatattt tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa
    30721 taaagccttt ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc
    30781 cgcaaaaacg gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt
    30841 cgttttccct ggctcactac tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga
    30901 caaaattatt ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg
    30961 taacgactat gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt
    31021 tcttggcaag ggcgaacagg tgctggccta tccgggcacc acgctctatt cgctggaaaa
    31081 agccctcaaa ccgctgggac gcgaaccgca ctcggtgatt ggatcgtcgt gtataggcgc
    31141 atcggttatc ggcggtattt gtaacaactc cggcggctcg ctggtgcaac gtggcccggc
    31201 gtataccgaa atgtcgttat tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa
    31261 ccatctgggg attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgatga
    31321 tcgcatcaaa gatgacgatg tgcgtcacga tggtcgtcac gcccacgatt atgactatgt
    31381 ccaccgcgtt cgtgatattg aagccgacac gcccgcacgt tataacgccg atccggatcg
    31441 gttatttgaa tcttctggtt gcgccgggaa gctggccgtc tttgcggtac gtctagatac
    31501 cttcgaagcg gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct
    31561 gaccgaaatc cgccgtcata ttctggctaa cttcgaaaat ctgccggttg ccggggaata
    31621 tatgcaccgg gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat
    31681 tgataagtta ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc
    31741 gatgctggag aaagtgaaat tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt
    31801 cggtcacctg ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga
    31861 gcatcatctg ctgttaaaaa tggcgggcga tggcgtgggc gaagccaaat cgtggttggt
    31921 ggattatttc aaacaggccg aaggcgattt ctttgtctgt acgccggagg aaggcagcaa
    31981 agcgttttta caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc
    32041 cgatgaagtc gaagacattc tggcgttgga tatcgctctg cggcgtaacg acaccgagtg
    32101 gtatgagcat ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca
    32161 ttttatgtgc tatgtcttcc atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc
    32221 gttaaaagaa cagatgctgg aactgctaca gcagcgcggc gcgcaatacc ctgccgagca
    32281 taacgtcggt catttgtata aagcaccgga gacgttgcag aagttctatc gcgagaacga
    32341 tccgaccaac agcatgaatc cggggatcgg taaaaccagt aaacggaaaa actggcagga
    32401 agtggagtaa aaattacgga tggcagagta tcgccatccg aattcactta atcgttctgt
    32461 gccgtctgcc ccgccgccgc catttgggcg gctttttgtt ttttatagct caacgctgct
    32521 gccggcacag gcatcacttt accggtttca atccaggtac gcaggcggct ggcatcggca
    32581 aaatgggtat atttgccaaa cgcgtccatc actaccagcg ccaccggttt attattgata
    32641 accgtacgca tcaccagaca atggcccgcc gcattggtaa agccggtttt ggttaactga
    32701 atattccagt tatcgcgata caccagatga ttagtattgc ggaacggcag cgtatacgtc
    32761 gggttagaga aggttgccat atcttcccgg gtagtactta actgcccgat caacggatac
    32821 tgtttgctgg caatgagcag tttggttaag tcacgggcag ttgaaacgtt atgcaccgac
    32881 aatccggtag gttcaacaaa gcgcgtgttg ttcattccga gcgatttcgc tttcgcattc
    32941 attgccttaa taaaggcttt gtaaccaccg ggataatggt gcgcaaggct tgccgccgcg
    33001 cggttttctg aagacatcag cgccagcaac agcatatctt tacggctgat ttcgctattc
    33061 agtcgtacgc gcgaatagac ccctttcatc tccggcgtct ggctgatatc cacttttagt
    33121 ttttcatcca gcggcagtcg tgcatccagc acaaccatcg cggtcattaa tttgctgata
    33181 gacgcaatcg gacgcaccag atccgggtgg ttcgaataga tcactttgtt ggtattcaga
    33241 tcaacaatca tcgcgctacc ggaggcaatt tccggttgtg aagcggtggt agcggctgcc
    33301 gttttcgcaa cggcctgcgg tgccaaaggc acagccagca tcagggccag gctaaataaa
    33361 gaaactcgaa atttcggcat gatgagcatt cagatagtgg ttcacgcgca cgggttgcgc
    33421 accgccggag taaggattta ctgaggctag cgacgccatc ataacgagca aaaagtgcga
    33481 tcgtcaaagg agaatcgtga ggaaatgctg cattgctgac atttacgcca gcaatgcaat
    33541 gtcaaagaac tttctagaac aggcgatacc cgtagcccca tagtataacg gttagggcga
    33601 gcagcacttc cagtaccagc acgccaatcg ccagcgtcga actggagaag ctaaggcctt
    33661 cctctttgtt gatattcagg aagctcggaa taccaaggta aagcaggtag ccggtgtaaa
    33721 acagcgccac cgtgccgacc agcgcacaca accagaccag cggataaagc gccaccagac
    33781 cgcttaaaaa cagcggagtt gcaacgtagc ccgcgaagac catgcagtgc gcaagtgacg
    33841 gacgctgcgg gtaattgcgc gccatccacc agatgacccg ccccatcacc gcaaccccag
    33901 cgagcataac gccataaaac aggacagcca gcgccagtcc ggtaaaccag gacaacttca
    33961 ggatagtgcc atcgccaaaa ttccagccaa tctgtgtagt gccaatgaag gcgcaaatca
    34021 ccggaatcgc cgccatcagc aaaacgtggt gggtgtaatg gtgagaaatc gtttcgtttt
    34081 cgcgattaat cacctgcatt tcacgatcgg gatgggaaaa cagtccccag acatggctca
    34141 taccgccccc ttgttgtgag ttcatgaacc tgacagttca agtataagtc agcttgtgat
    34201 tattttttgt tcgctcatgt aatttgcacc gtctgatgta cgtccttttt cagcgtgaat
    34261 gatttccagg gtgtatgctt acaggcaacc aaagggagac agactggcct atggatctca
    34321 atacactcat ctcacaatat ggttatgccg cgctggtgat cggtagcctg gcggaaggtg
    34381 aaaccgtgac tttgctggga ggcgttgcgg cgcatcaggg gctattaaag ttcccgctgg
    34441 tggtactttc tgtggcgctt ggcggcatga ttggcgacca ggtgctttat ctgtgcgggc
    34501 ggcggtttgg cggcaagctg ttacgccgtt tctcgaaaca tcaggataaa attgagcggg
    34561 cgcagaaact tatccaacgc catccgtatc tgtttgtcat tggtacgcgc tttatgtatg
    34621 gctttcgggt tattggcccg acgctgattg gtgccagcca gctgccgccg aaaatctttc
    34681 tgccgcttaa tattctcggc gcatttgcct gggcgttgat ttttaccact atcggttacg
    34741 ctggtggtca ggtgattgcg ccgtggttgc acaatctcga tcagcatttg aagcactggg
    34801 tctggttgat tctggtcgtg gttctggtgg taggcgtgcg ctggtggctg aaacgacgcg
    34861 ggaagaaaaa gccggataat caggcgtaaa accattgccc tggataaggc gttcgcgccg
    34921 catccgacat ccggtgcacc atgcctgatg cggcgcttgt gcgtcttatc atgcctacca
    34981 aaattttacc cattgaaata tccacacacc tcactgcgct attctgggtt gaactgcgga
    35041 ttcgccaaca taaagccgcc atccactatc aacgactgcc cggtggtgta atttgcgccc
    35101 tccgaacaaa gccacgccac caggctggca atctcatacg ttgcgccaaa acgccgcaga
    35161 ggaatcgaag gctccgcgtc gggcttcacg tcgctatcat ccatgccgtt cattggcgta
    35221 gcgatcgccc caggtgcgac tgcgttcacc aaaatcttat gcctgaccaa ctccagcgcc
    35281 attgctttgg ttaacccgcc gagcgcatgt ttagcggctg tgtaggcgct ggcatccggc
    35341 agcggcgtat gttcatgtac cgacgtaatg ttgatgatgc gaccaccctg cccttgtttc
    35401 accatctggc gggccgcaat ttgtgagcat aaaaatgcac catcgacatc aacggtaaaa
    35461 atcttgcgcc actcatcgaa agccatatca agaaacggcg ctttggtcat tgcgcccgca
    35521 ttattcacca gcacatcaat acgccccagc cgttgaatga gtttctccag cgcctgtgca
    35581 ccttctggca gtttaccgag atccagctgc acaatctccg cacgtacgcc gtggctaact
    35641 acctcacgcg cggtatcttt tgccccttct tcatctgagt gccaggtaat gccaatatca
    35701 aacccctgct gcgccagtaa taacgcgcac tctttgccga tccccgaatc ggaggcggta
    35761 ataatcgcaa cctgtgccat cgagttctcc acttaacgct gaataaacgt taagtataga
    35821 aggcgcatat catcagcgtt tgtaaccccc gcccaacgca ccagtgagtt gaatggaagc
    35881 atccagccac tgcccttgca ataacaggcc attggcccgc tcacgcagcg cggggattct
    35941 ggcttcgctg acgcgggaac cagcaatgat gcccgcgtta aagcgcgcct gcgcaagacc
    36001 taccacacgc aaggcatcgc gctcaatttg cgcctgatgc tggtttttct ccgcaagtgt
    36061 ctgaacctga ctggctgccc gcgccacgtc attcaccgct tcaaccaccg ctttgttgta
    36121 gctggcgata gacaagttgc tttcggcttt tgcgatatcg agattggcgt taagacgacc
    36181 actatcgaaa atgggtagcg tcaggcctgc cgtaacgccc atttgctgcg cggaatgacg
    36241 gaacagatcg cttaagtgca acgcatcctg ttgcaggaag gccatcaggt tgatgtcagg
    36301 ataaaatgcc gcttttgccg catcaatggt gcttagcgat gactcaacgt accagtgcgc
    36361 cgcctgcaaa tctgcccgcc gggccagtaa ggagtacccc agttcatcag gaagctggct
    36421 tgccactttc ggcaacgcga ccggtttaag cttcaatgac tttgtctggt tatttgtaag
    36481 tgcgcttaac cgtgcctcaa taattttcat tttccccgcg acatcgttga gctgctgccg
    36541 ggttttgctg gcattaatat cggtttccac accttcaact gaagaagtaa tcccgttctg
    36601 atatagctgg cgatcggtcg cgataatggt gttctgctct ttttctattt gctgcaagac
    36661 cgtgtttaac gccgcctggg tttgccactc ccagtacagg cgggctacgc tgccagccag
    36721 caattggcgg gtttgctcgc gttccgccgc ccgtgcttta accgtaccca ggcgggcagt
    36781 aacctccgcc cgattctttc cccagatatc gagatgccag cccgccgtta agccaaaagt
    36841 tccgttggtg taccacggac cggtcgtacc tgcggccgga tcgttcagag caaacggccc
    36901 cattaagcct tctgccgaca ttttttgccg ctccatatcc gcagaaaagt cgatctgcgg
    36961 accatcctga gtagcaattg ccttcgcctg ggcttcagct agctgaatgc gctgttcagc
    37021 cacctgcata tccggtgcgt tctgtagtgc attgttaatt aaggaagtga gttgattatc
    37081 gtgatactcc agccaccatt ggctgtctgg ccaaccattt ttcagcgccg tgggtaatgc
    37141 ggtgtcaact tgtgcagcgg gcgtttgctg gcttaacgcc tggcgggttt catgcatagg
    37201 cccacacccg gccagcatca gtaacagcgg aaaacaggcg atggcgggat aaaaggaatc
    37261 acgattcatg ggggaataat caggtaagaa aaggtgcgcg gagattaccg tgtgttgcga
    37321 tatatttttt agtttcgcgt ggcaatacat cagtagcaat aaaacgacat atccagaaaa
    37381 atatacacta agtgaatgat atcttccgat ttatcttaat cgtttatgga taacggcaaa
    37441 gggcttcgtt ttttcctata cttattcagc actcacaaat aaaggaacgc caatgaaaat
    37501 tatactctgg gctgtattga ttattttcct gattgggcta ctggtggtga ctggcgtatt
    37561 taagatgata ttttaaaaat cagatatggc atataagttg tcaggtcagg aactagatat
    37621 tttgtgtact tgatttgcaa acagctccgg tgttggtggc gtcaaaatcg ctgaagcgtt
    37681 ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta caaggacgtt
    37741 acctcgcgcc cgacccggta gacgaagggg ataagctgaa ggtaccgcgc agcggcgatt
    37801 ttgttcgcca gagcccgggg gtgcaggggg aggaggcgat tgtcctcccc ctgcgcgctc
    37861 cttgcgccag tggcaatatg tggcttagct catgaaagga gcgcaacaag atgatgaagc
    37921 tgaataagcg attcattgtc tctgataaac tgcgattcca ctattatcgc agcttatcga
    37981 tatcaattgc ctgaatagcc cttgcaatat caggggaatt attcaacacc cgaacatgct
    38041 gaaataattc cgttgcttca tcgtattctt tacgcaaata actcaaccac tgtttaatcc
    38101 gcgcaacgtg atataacccg gtatcgccct gcttttccag acgggtatat ttttgcagca
    38161 aagcaaccac ctccggccac ggcattcgcg gttcgttata ttttactacc cggctcaggt
    38221 tgggaatatt gagcgccccg cgaccaatca tcactgcgtc gcagccgctg atcgcaatgc
    38281 attgttgcgc gctctgccag tcccagattt cgccgttggc aatcaccgga atattcagcc
    38341 gctggcgaat ctcgccaatc gcctgccagt caatatgctc cgcgcggtaa ccctgctctt
    38401 ttgtccgtcc atgcaccacc agctccgtag caccagcctg ttgaaccgca tcggcgattt
    38461 caaatttctt ctcaccgctg tcccagccca gacgcacttt cacgctgacg ggcaaatgcg
    38521 ccggtacagc ttcacgcatc gcttttgcac cctggtagat gagttcagga tctttgagta
    38581 acgtcgcccc accaccgcta ccgttgaccg ttttcgacgg gcagccgcaa ttgagatcca
    38641 cgccccagga acctaattcc accgcacggg cggcgttctc tgccagccat tgtgggaact
    38701 gacccaacaa ctgcacgcgc accagcgtac cggatggtgt ccggctggcg ttttgtagct
    38761 cagggcaaat gcgatgaaaa acttttaccg gcagcagttg atccaccacg cggacaaact
    38821 cggtgatgca cagatcgtag tcgttaactt cggtcagcaa ttcacgcacc agagagtcaa
    38881 gcacaccctc catcggagcc agtaacacac gcatatcttc acccgcaaaa aaatgaggcg
    38941 ctatgttagc gcctatggtc aacggattaa aggtctgcaa agagggattg ctgcaaactg
    39001 taatccctct ctatgcttga tgtatgcctg agttcgttgc gcattgcgta atgccgggga
    39061 gcgaaaaaat aagcgctgat ttcttaatgt gatcggtagc acgttttacg aattaattgt
    39121 atgatgaatc catctcatct ggggtgttga ttatgagtaa gacactgaac attatctggc
    39181 aatatttacg cgctttcgtc ctgatttatg cctgcctgta tgcaggcatt ttcattgctt
    39241 ccctgctacc ggtaaccatt ccgggcagca tcatcggtat gctgatcctg tttgtcctgc
    39301 tggccttgca aattcttccg gcaaaatggg tcaatccggg gtgctacgta ctgattcgct
    39361 atatggcgct attgtttgtg ccgattggcg taggcgtcat gcaatatttt gatttgctcc
    39421 gcgcacagtt tggcccggta gtggtttcct gtgcaatcag tacgctggtg gtttttctgg
    39481 tggtgagctg gagttcgcaa ctggtacacg gtgaacgtaa agtcgtaggt cagaaaggat
    39541 cagaagaatg atggcgaata tctggtggtc attaccgctg acgttgattg tcttttttgc
    39601 cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt aacccgttgc tggtagcaat
    39661 ggtggtgatc attccttttt taatgctgac cggcatctct tacgacagct actttaaagg
    39721 tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc gcgctggcct atcctttata
    39781 tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc atcaccatct gttttatcgg
    39841 cagcgtggtt gcgatggtga cggggacttc cgtggcattg ttgatgggcg cttcaccgga
    39901 aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct attgcaatgg cggttggcgg
    39961 cagtattggc ggtattccgg caatcagcgc agtttgcgtg attttcgtcg gcatcctcgg
    40021 cgcggtattt ggccatacat tgcttaatgt gatgcgtatt cgtaccaaag ctgcgcgcgg
    40081 tctggcaatg gggactgcct cgcacgccct cggtacggcg cgctgcgccg agctggatta
    40141 tcaggaaggt gcatttagtt cgctagcgct ggtgttatgc gggataatta cttcgctgat
    40201 cgcaccgttc cttttcccga ttattctggc agtaatgggc taaaatttgc gatgcgtcgc
    40261 gcatttttga tgtaagtttc acgcgttgca taattaatga gattcagatc acatataaag
    40321 ccataacggg ttcgtaaact gttatcccat tacatgatta tgaggcaacg ccatgcatcc
    40381 acgttttcaa accgcttttg cccaacttgc ggataacttg caatctgcac tggaacctat
    40441 tctggcagac aagtacttcc ccgctttgtt gaccggggag caagtctcat cgctgaagag
    40501 cgcaacgggg ctggacgaag acgcgctggc attcgcacta cttccgctgg cggcggcctg
    40561 tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt gcgcgcggtg tgagcggaac
    40621 ctggtatttc ggtgccaata tggaatttat tggtgcgaca atgcagcaaa ccgttcatgc
    40681 cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa aaagcgcttg cagccatcac
    40741 cgttaactac acgccttgtg gtcactgccg tcagtttatg aatgaactga acagcggtct
    40801 ggatctgcgt attcatctgc cgggccgcga ggcacacgcg ctgcgtgact atctgccgga
    40861 tgcctttggg ccgaaagatc tggagattaa aacgctgctg atggacgagc aggatcacgg
    40921 ctatgcgctg actggtgatg cgctttctca ggcggcgatt gcagcggcaa accgttcgca
    40981 catgccttac agtaagtcgc caagcggtgt cgcgctggaa tgtaaagacg gtcgtatttt
    41041 cagtggcagc tacgctgaaa acgccgcatt caacccgact ctgccaccgt tgcagggagc
    41101 gttaattctg ttgaatctca agggttatga ttacccggat atccagcgcg cggttctggc
    41161 agaaaaagcc gatgcgccgt tgattcagtg ggatgccacc tccgcaacgc tgaaagctct
    41221 cggctgtcac agtatcgacc gagtgcttct cgcttaagcc tggtgccgga tgcggcgtga
    41281 acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa ttcccggcaa acagtttgcc
    41341 gtttcttgcg caaaaccagc gggtaaagta gcctgatgga aattttcctt agatcgagtc
    41401 tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc tgatcggctt gctgctgttg
    41461 actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg cgccttatat ctacgacgaa
    41521 ttgcaggatc tcccctaccg ccaggtcggt gtggtgctcg gaacagcaaa atattatcgt
    41581 actggcgtaa ttaatcagta ttatcgctac cgcattcaag gagcgattaa tgcctataac
    41641 agcggtaagg taaattatct attactgagc ggcgataacg cattgcaaag ttataatgag
    41701 ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg acccatcaga tattgttctc
    41761 gattacgcag gctttcgtac gctggattcc atcgtgcgta cacgcaaagt tttcgatact
    41821 aatgatttca ttattatcac ccaacgtttc cactgtgagc gggcattatt tattgcgctg
    41881 catatgggga ttcaggctca gtgttatgcc gtaccgtcac cgaaagatat gctgtcagta
    41941 cgtattcgcg aatttgccgc ccgtttcggt gcgctggctg acctttatat ttttaaacgt
    42001 gaaccgcgtt ttttagggcc gctggtccct attccggcta tgcaccaggt gccggaagat
    42061 gcgcaggggt atcccgccgt cacacccgaa cagttacttg aattacaaaa gaaacaagga
    42121 aagtagttat ggatgtacag cagttttttg tcgttgccgt ttttttcctt attccgatat
    42181 tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc gattgataaa cgggttaaaa
    42241 atgcaccgga accggtgtat gtctggcgag caaaaaatcc cggactcttt ttcgcttata
    42301 tggtggcata tatcggcttc ggaattttat ctatcggcat gattgtttat cttattttct
    42361 atcgttaata ttttctcttt tctgttaatt attgagaata attattactt cactcaataa
    42421 gctgcggata taacattctg aaccgcagct atttgtgaat cctttcacag tataaatttc
    42481 cccacgcgca tagccttaat atcagcacgc aatatttata ctctaaataa ttcgagttgc
    42541 aggaaggcga caagtgagtg agtccccagg agcttacata agtaagtgac tggggtgagc
    42601 gaacgcagac gcagcacatg caacttgaag tatgacgagt atatttagcg ttcgccttaa
    42661 ttactcatag cattaaggaa gatcacatgc cgcaacaaaa ttatctggat gaactcactc
    42721 cggcttttac gcctttactg gcgattaaag aagcctctcg ctgtttatta tgtcatgacg
    42781 ctccctgtag tcaggcttgc ccggcacaga ccgatccggg gaaatttatt cgctcaatct
    42841 actttcgtaa ttttaaaggc gctgccgaga caattcgcga aaataatgcc ctcggtgccg
    42901 tttgtgccag agtgtgcccg acggagaaat tatgtcaaag cggttgtacc cgtgccggtg
    42961 ttgatactcc cattgatatc ggtcgcttac agcgttttgt tactgatttt gaacaacaaa
    43021 ccgggatgga aatttatcag cccggtacta aaacgctcgg caaagtcgcg attattggcg
    43081 caggtcctgc cggattgcag gccagtgtaa cactgacaaa ccagggttat gacgtcacga
    43141 tttatgagaa agaagcgcac cccggtggct ggttgcgtaa cggtattccg caattccggt
    43201 taccgcaatc agtgctggat gcagagatcg cccgtattga aaaaatgggc gtgaccatta
    43261 agtgcaacaa cgaagtcggt aacacactca cccttgagca gctgaaagca gaaaaccgcg
    43321 cggtactggt caccgtgggg ttatcgagcg gttccgggtt atcgctgttt gagcatagcg
    43381 acgttgagat tgccgtcgac ttcctgcaac gtgcacgaca ggcgcaaggc gatatcagca
    43441 ttccacaaag cgcattaatt atcggcggcg gtgatgttgc gatggacgta gccagcacgc
    43501 tgaaagttct cggctgtcag gcggtaacct gcgtagcgcg tgaagagtta gatgagtttc
    43561 cggcaagcga aaaagagttt gccagcgccc gggagctggg cgtttcgatc atcgatggat
    43621 tcacgccagt agccgtggaa ggcaataaag tcacgtttaa gcatgtacgg ttaccgggcg
    43681 aactgacgat ggcggcagat aaaattattc tcgccgtcgg tcagcatgcc agactggatg
    43741 cctttgcgga gttagagccg cagcgtaaca ccatcaaaac acaaaattat cagacccgcg
    43801 atccgcaagt ctttgctgct ggcgatattg ttgagggtga caaaaccgtg gtctatgccg
    43861 tgaaaaccgg gaaagaagcc gccgaggcga ttcatcacta tttagaggga gcttgctcat
    43921 gttaacgaaa gatctttcga ttactttttg cggcgtgaag tttcccaacc cgttctgcct
    43981 ctcttcttcg cctgtaggca actgctatga gatgtgtgcc aaagcctacg acacaggttg
    44041 gggcggcgtg gtgtttaaaa cgatcggctt ttttatcgcc aacgaagtct cgccgcgttt
    44101 tgatcatctg gtgaaagaag ataccggttt tatcggcttc aaaaatatgg agcagattgc
    44161 tgaacatccg ttggaagaga atctggccgc cctgcgtcag ctgaaggaag attacccgga
    44221 caaagtattg atcgcttcga tcatggggga aaatgagcag caatgggagg agctggcgcg
    44281 cctggtgcaa gaagctggcg cggatatgat cgagtgtaac ttctcctgtc cgcaaatgac
    44341 ttctcatgcg atgggtagcg atgtcgggca aagcccggag ctggtagaaa aatattgtcg
    44401 ggcagtgaaa cggggttcca cgctgccaat gctggcgaag atgacgccga atatcggtga
    44461 tatgtgcgaa gtggcgctgg cggcgaagcg cggcggcgca gatggcattg cggcgattaa
    44521 caccgttaaa tccatcacca atatcgatct taatcagaaa atcggtatgc cgatcgttaa
    44581 cggaaaatcg agtatttccg gatattccgg taaagcggta aaaccgatcg ccctgcgctt
    44641 cattcagcag atgcgcaccc atccagaact gcgcgatttc ccaatcagcg gtatcggcgg
    44701 cattgaaacc tgggaggatg cggctgagtt tttattgctc ggcgcagcaa cgttacaggt
    44761 gaccaccggc atcatgcagt acgggtatcg gatagtggaa gatatggcaa gcgggttgtc
    44821 gcattatctc gccgatcagg gatttgattc gctgcaggag atggtaggtc tggcgaataa
    44881 caatattgtc ccggcggaag atttagaccg cagttatatt gtctatcccc gtatcaatct
    44941 tgataaatgt gttggctgtg gacgctgtta tatttcctgt tacgacggcg gtcaccaggc
    45001 gatggaatgg agcgagaaaa cccgcacacc gcattgtaat accgagaaat gtgtgggttg
    45061 tctgctttgt ggtcacgtct gcccggtggg ttgtattgag ctcggggaag tgaagtttaa
    45121 gaaaggcgag aaagaacacc cggtaacgtt gtaaaaccag agacgcatcc ggcatttggc
    45181 accgattgcc ggatgcagcg catccggcaa tatccacaat cacatcactt cttacgcgca
    45241 tatttcagtg aatccagcgc tacggcgaag ataataatcg cccctttgat gatgtactgc
    45301 cagtatgggt ttacgccgat atacgtcagg ccatagttga tgacggtaaa aataattacc
    45361 ccggtcacca cgccaatcac cgtccccaca ccgccgctga acgatacacc gcctaccacg
    45421 cacgccgcga tagcatccag ctcatacata aagccgaggt tgttggtggc agagccgata
    45481 cgtccggctt ctaacatccc gccaaaggca tagaacacgc cagacaacgc gtagatcatc
    45541 agcaggttca ggccgacgtt gacaccagat acttttgccg cttccgggtt accgccaatg
    45601 gcaaaaatgt tcttaccgaa gcgggttttg ttccacaaca cccagacgaa cgccaccgca
    45661 atcaacgcgt agaaggtgat gtaagagaga cggaaactcc ccagcgcgac aaagccctga
    45721 gcaaaggtag agaagccact gtcaaaacca gaaattggcg acgccccgac aaagtcgtaa
    45781 tagagcgagt tgattccata gacgatgatc atcgtgccga gcgtggtaat gaacggcgtc
    45841 acgttgagat aagcgataat cagaccgttg atcaaaccga tcaccgcacc aatggcacag
    45901 acaatcagaa taaccagcgc aatcggcatc gtcgccattt ccgggaacac tttgttggcg
    45961 ttatccatgg actgcaataa tgtcgcagcc accactgccg ccagccctac ctgacgacca
    46021 gcagaaagat cggtcccctg ggtgacaatt aaccctgcca caccgagcgc gataataata
    46081 cgcaccgatg actgggtgag aatattactt aagttcaaca gacttaaaaa tgttgggtcc
    46141 tggaaaataa taatcgccag caaaactaaa agaacgacgt aaataccgcc ctctttcagg
    46201 taagtaagaa aacttttctt atttaacgca ctcatgggaa gcccctaatc ttaaaggtgc
    46261 aaagacgcaa gacgcagaat ttcgttttgc gttgttgttt ttgtatcgac aattccggaa
    46321 acgagaccat tgctcatgac cagaatacgg tctgttatcc ctaacaactc aggcatttca
    46381 gaggagataa taataatccc cttgcctttc ttcgccagtt cggcaattaa ctgataaatt
    46441 tcaaacttcg ccccgacatc aataccgcgc gtcggttcat cgagcattaa tatttctggt
    46501 tgcgttagta gccagcgacc aataatcacc ttttgctgat taccaccgga gagcgaacca
    46561 atttgcgtcc gatgacccgg cgtttttacc cgcatcgaat caatcaccca ctgggtatcg
    46621 cttttcatcc gcgagttatc cagtaaacca actttatttt tgtagttgcg aatattggaa
    46681 attaacgagt taaaactaat atccagatag gcataaattc ccgttgagcg gcgctcctca
    46741 gttaccagtg caaatccgtg gtttatggct tcgttggcat tatggttatt gatctgtttg
    46801 ccgtgcaacg taatggtgcc agccgatttc tcgcgaatac caaataacgt ctcaacaata
    46861 tcggtacgtt tcgcccccac cagcccggca ataccgagga tctccccttt atgcagatca
    46921 aacgagacat cgcgaatcga cggctggcgc agtgacgtta ggttacgcac ctcgaggatg
    46981 acttcgcccg gcttgttttc tttgtcagga aaacgctgat taagagaacg cccaaccatc
    47041 atggcgatga tcttgtccat cgtcagtcct gccagcggtt cggtggcgat ccactgaccg
    47101 tcgcgcaata cggtaacttc atcacataac tggaagattt cttccatttt atgcgagata
    47161 taaacaatac cgcagccgcg ctcttttaat ttacgaataa tagtgaacag atgattgacc
    47221 tctttttcgg ttaacgaaga agtcggttca tccataatca caattttcgc gttataggaa
    47281 aacgctttgg cgatttcgat catctgcatt tgcgaaacgg ataatgtgcc gacgcgcgca
    47341 cgcggatcga tatcaatatc cagttcatca aaaatcgctt tggtttcgcg gtacattttg
    47401 tcctgatcga caaacatgcc tttggtggga tatcgcccca gccacatgtt atccatcacc
    47461 gaacgttgta ataccaggtt taactcctgg tgtaccatcg aaataccatt ttccagtgcc
    47521 tctttggcgg aatggaaatc gatctcttta ccctggaata aaatggtgcc ggagtctttt
    47581 tgataaatac caaacaggca ttttaataat gtcgattttc ctgcaccgtt ttcccccatt
    47641 aatgcatgga tagaatgtgg ccggactttt aaattaacgt tatcaagtgc cttaacacca
    47701 ggaaaggact tgttgatacc gctcatttcc aacaagtatt ccccggaaga cggagtcgtt
    47761 gagctgacca tataatttta ccttgttggc catacaataa gggcgcagta aacgactgcg
    47821 cccaatcagt cttatttctt gctgaattca gccaggttgt ctttatctac gccaacataa
    47881 ggtacgcgga ccactttgtt gtcgattttc cagttggtgc catcagccgc acctttgcca
    47941 tcggccaggt ttttcgccag atcaaaggtc gctttcgcct ggttgttagc atcgttcagt
    48001 actgtgcccg ccagtgcacc ggatttcacc agcgccagcg cttctggcag cgcatcgacg
    48061 ccaaacaccg gaatgctgga cttgttgtgt gctttcagtg cttctaccgc gcccattgcc
    48121 atcgcatcgt tgttggcgat aaccacttcg attttgttgg cgttcgggcc agacagccag
    48181 gcgtccatct tatctttcgc ctgagcggta tcccacatag cggtatctaa ctgtaactgt
    48241 tcagttttaa tgcctttgtc gttcagctct ttaatcacgt aagtggtacg tgcttctgca
    48301 tccggatggc ccggttcacc tttcagcaat acgaattgaa tctgaccgtc tttgttcaga
    48361 tcccaaccct gattcgccgc ccagtgttta gcaatcaaat cgccctgaat aatgccggac
    48421 tctttggaat cagtgccaac gtagtaggct ttgtcgtagc tatccagcgc cttacgagac
    48481 ggttctttgt tgaagaaaac caccggcacg ttttgcccac gcgctttctc aatcaccgta
    48541 cccgcagctg ccgggtcaac caggttgatt gccagtgcct tcaccccttt cgccagcaat
    48601 acgtcgatct gatcgttctg cttggactgg tcattctgag aatcattcat cagcagctga
    48661 acatctggcg cggctttcgc atcttgctca atagccttgc gcactacaga cataaagtta
    48721 tcgtcgtact tatagattgt tacaccaatg cgagtatcag cagcgtgtgc agcggcaccg
    48781 aataacatgc tggccatcac agcagacagg gttaacacct tcttattcat ggtatctccg
    48841 gtttttctta tgcagggtag tgcttgagat aaatgctcgg cggggcagta gagttaatga
    48901 agtgttactg aacgccgaag ctcacttttt aaaattcgtt cttccatgcc cggtaacgct
    48961 ccagaaaacg gctttaattg ttgttatgac gctattacct cggtgaaagt gattattcac
    49021 tgttacatac gggttacaac gttaaaacgg tgcaatcata gctatcacat tgttaagata
    49081 ctgtgaaatc actcacagat tgaaagcggt tacatcgcct gatttgttga gttagtgatc
    49141 gtcgccgcat tctggcgtgt tgcgacagaa tggcgacgca ctaacgtcgg cataaaacag
    49201 tggctagcac gaggagcaat attgcctgct gccccctgca aggccagttc ggtggctaat
    49261 ttcgccattg aagcaatggg ataacgcacg gtcgttaatt gcgggtcggt gtaacgggcg
    49321 atgggaatat catcgaaacc gatgattgag agatgtaacg gaatcgcaat gccattatct
    49381 tttaatgctg tcagtgcgcc agcagccata ttgtcgttat aagcaaatac agcggtaagt
    49441 tgtagattgc gccccagcag ttcaaccatc gccgcctcac cgcccggcat gtccggcgta
    49501 ccagtgccaa tccagctttc cggcggaata atatcctgct ctttcaacgc actcatccag
    49561 cctgctttac gcatggcgtc atcttcaatg ccgtgactgg aagaaagata accaatacgt
    49621 tgatgaccgt tattcagcaa catgcgcgtc gccattcggg caccgctgag attatccagg
    49681 caaacgcaac gatgggcgta ccccggcaca acgcggttga ttaacaccat accgggaatg
    49741 ttatccataa attgcgccag ttcatcgtca ctcaatgctt ttgagtgaac aatcaacgca
    49801 ttacaacgct ggcgaattaa cacctcaatg gcgtgacgct ctttttccgc ttcatgatag
    49861 ctattgccga ttagcacgta tttctgatgc tgctgagcga ccagatccac cgcttttacc
    49921 agcgcgccga aaaacgcatc agaaacgtcc atcaccacca cgccaatggt gtcgctaacc
    49981 tgagttgcca gcgcctgggc attggcgttt ggccgataat ccagctcact cacggctttc
    50041 attactgctt cacgcgtgtc ggcactgacc agcgtgctgt tattgagcac ccgggaaacc
    50101 gttgccacag agacgccagc ctgacgcgct acatcacgaa tggtgatcat attcactacc
    50161 tgttatgaga ttgcagtaaa tgactgcttg ctggcggcta ttttgtcagc actgaataca
    50221 ggacttcgtg aatcgagtca cagcaatgga aacggttaca gccgttttgt taacgaatgt
    50281 gatccagatc gttatcttga tgttttagat attgccgttc cggcagcacg caaagttaac
    50341 tgacgccaga gccattccac cggcccctgg cggaagtaac gcagccagat aacagagaag
    50401 aggatattcg ccagccatac cggaataaca aacgccagca gctccaggcg gtcaaaatgc
    50461 ataaacaaac cgaggtggta aaaaagcatg gtacaaatca gcgtttgcaa tagatagttg
    50521 gtcagcgcca tccgtccgac gcaggcgatc gcaagcacca gcttaaagcg gctcaattgc
    50581 ggccagaagc cataaaacag cgacgcatag ccaatcgcct gaaacggcgc actcagttcc
    50641 cgcggcattt gaagtaagaa ggcacaccag cgatacgccc agtccagctg ccattgcagg
    50701 gcgatggcag gaaggttaat ggtcacccca atcgccacca gcacaaaacc agtacgacga
    50761 tagtgacgta agctgaactg ccctttcagc cagccgctgc gcatcagcgc tgcaccaatg
    50821 agcatcatcc cagccagttg ccagccatac tgtgcgccca gtgccagtaa actgttgcca
    50881 acaccatcgg cacgattact gatcgcttca acgccgccgt gaagcttcca gtatttttca
    50941 tacaaaatag ccgatgcatc cggcgtccag gcgcggctgg tctggctgtc ggaaatcagc
    51001 cccaataaca gcaaaacgcc aagccccacc agataaagca tgacgcctgt attaaacagg
    51061 ctttttaccg atggcgcatc gcgcaccagc cgccagcaga ttaagcccac cagcccgtaa
    51121 gccagcagaa tatcgccgtc ccagaacaat aaaccgtgaa taaagcccaa taagactaac
    51181 agcgttaacc gcgactggat ccagcgtctg ccacggggca gcaacatttg cagtccagca
    51241 ccaaacagca gcgcaaaaag cgtgaggaat ttcacctggg cgatgagatc aagaaatgcc
    51301 caggtccatg catcctgcgg cgtaatagca ccgtaccagg cgggattgag ataagccgcc
    51361 tttggtagcc caaaggcgct gatgtttaat agcaggatcc ccagaatggc gacgccgcga
    51421 acaaaatcga gcgtgacgtt gcgctccatg gttcctgcct tttaatcagt tgtgatgacg
    51481 cacagcgcgc agaaactcgt ggcgcgtatt ctgactggat ttgaacaatc caccaagaga
    51541 ggtcgttgtt gtggcactgg ttgcatcgcg gatgccacgc gccttcacgc agtaatgcac
    51601 cgcgtcgatc gagacagcca cgttattggt gcccagcagc gtttgtagcg caataagaat
    51661 ttgctgcgtc agacgttcct gcacctgcgg acgctgggca aagaactgca cgatacggtt
    51721 aatttttgac agaccgatca ccgaatcttt cgggatataa gccaccgtcg ctttgccatc
    51781 gatggtaaca aaatggtgtt cacaggtact ggtcagagtg atatcgcgca cggtgaccat
    51841 ttcatcgacc ttcattttgt tttcaatgag ggtgattttc gggaagttgg cgtaatccag
    51901 accggagaaa atttcatcga catacatttt agcgatgcga tgcggcgttt ccatcaaact
    51961 gtcatcagcc aggtcgagat tcagcagctg catgatttcg gtcatatgac cagcaataag
    52021 gcttttgcgc gtttcgttat ccatttcatg cacgggcggg cgcagcggtg tttccagtcc
    52081 tcgcgcaact aacgcttcat gaaccagggc cgcttcttta ctgagtgatg gcatttatga
    52141 tttctcctgc aggtgtgacg cctccgccct gcgtgggggc aaagttatta agctgattta
    52201 cagcttgatt attgtgcgtg aggcggcgca cataatccag tattcacagc gataattatt
    52261 gtaattgccg ctgcctttca tcagcagatg ttaaaacatc gttatgcaaa tacggaagtg
    52321 aaagttactc acagcacatt gaataaacgg tatgatgaag aaattgcaaa caacacaaca
    52381 aggagccacg catggaaatg ctcgaagagc accgctgttt tgaaggctgg cagcaacgct
    52441 ggcgacacga ctccagtacc ttaaactgtc cgatgacgtt cagtatcttt ctcccaccac
    52501 ctcgtgatca caccccgcca ccagtgctgt actggctttc cggattaacc tgcaatgacg
    52561 agaacttcac catcaaggcg ggtgcccagc gcgtggcggc agaactgggg attgtactgg
    52621 tgatgccaga caccagcccg cgcggcgaac aggttgccaa cgatgatggc tacgatttag
    52681 gccagggcgc ggggttttat cttaacgcca cgcaaccgcc gtgggcgacg cattaccgga
    52741 tgtatgatta tctgcgcgat gaattaccgg cgctggttca gtcgcaattt aatgtcagcg
    52801 accgctgcgc cattagcggt cattcaatgg gtggtcacgg tgcgctgatt atggcgctga
    52861 aaaatccggg taaatacacc agcgtttcgg cctttgcgcc aattgtgaat ccgtgcagcg
    52921 tcccgtgggg aatcaaagcg tttagcagct atttaggtga ggacaaaaat gcatggctgg
    52981 aatgggacag ttgcgcgctg atgtatgcca gtaacgcgca ggatgcgatc ccgacgctta
    53041 tcgatcaggg cgataatgat cagtttcttg ccgaccagtt gcaacctgcg gtactggcag
    53101 aagccgcgcg ccagaaagcg tggccgatga cgctgcgtat tcagccggga tatgatcaca
    53161 gttactactt catcgcctct tttatagagg atcacctgcg cttccatgcg cagtatttac
    53221 tgaagtgaaa gtccgcccgg ttcgccgggc atcttctcat cagaagcgat aatccactgc
    53281 cataaagtaa cgacgtccgt cttcgttata gctgtagtcg tcacgactga gatccttgtc
    53341 gccaaggttc agcacgcctg cgcgcagttt gacgtcttta gtcacctgcc aggccgcgcc
    53401 ggtattccag atggtataac cgcccggtgt tttagccgtc gcgctgtcgg cgcgtttctg
    53461 cccggtatag tgaccagaaa catagaatga ccagtcttcc agcgccagcg gtttccagtc
    53521 cagcgtaccg ttagcagtat ggaacggcag atcggatagc ggtttgtttt cgccgttgct
    53581 gacatcacga ccatcgttgt aggtgtagtt gatcgacagt ttccattcat cgttgaacgg
    53641 aattttcagt tcggtttcca cgccctgaat acgagctttg ttaacgttgt agtagctaaa
    53701 taccggtatg cgccgtccgt tagcgcccgt ctcaaaacca acaaagtttt ggtagcccgg
    53761 tgcagcgttg acgtcagacg tacggctgat gctgatacga tctttcacat cgttacggaa
    53821 aacggtaacg ctggattcaa ccccttccag ccagccttct tcacccatgt agtaaagccc
    53881 cagctcccaa ctttcgctgg tttctggttt cagatctggg ctacccacaa tcttacatgc
    53941 gccacggcag gaattgctcg tccagtcagg gctaagttgc aacagagaag gtgctttaaa
    54001 tgccgtcgcc cagccccctt tcaccgttac ggtgtcggtg gcgttataaa ccaggtaggc
    54061 acgcggactc cagtgttcac cgtaggtttc gtgatcgtcc atacgcacgc cggtcgtcag
    54121 cgccagcggc tcgaagatcc gccattcatc ttccacaaac agcgcgtact ggctggcaga
    54181 cgttttggag ctggttcccc cggtcaggtt caccgcatcg ctaagtttgt cgtgacgcca
    54241 ttcaccgcca accgtgagaa actgattaat tgccgtcagc ggcaacgtgt atttgccgtc
    54301 gaccgtattg ctttcggaag ttatcgggct gctgttgcca gggtttttgt tctcgacttt
    54361 ctcaccgtag tatttcagtt cgctggtgcc gtaatcccaa cgcccattat ggctgacgga
    54421 gtagttctgg cgttccaggc ggtttttgtc cagcgagtcg gaatcacgat cctgacggtc
    54481 gaaaccgtat ccggcagtaa aatcgtgatt ttgattcggt gtccaggcaa attcgacatt
    54541 gccgtcgcgg ctggagaatc cttcaatacg cggcgtttct ccggtatcgg tggtcgttga
    54601 gttttgcggg tcatcctttt cacgttttgc caggctgccg taagctttca ttcccagcac
    54661 accatcaatt aatggtccac tggtaaagaa ctgaccgtta taggtatcac cgcgatcgcg
    54721 atgttcctga atggtggtat cgacggtaac ggtacccgac catttctgac cgattttttt
    54781 ggtgatgata ttcactacac cgccgagcgc atcggagccg tatagcgacg acatcgggcc
    54841 acgaaccact tcaatacgtt cgatggaatc gaccgggatc cagttcagat cgaaatcatt
    54901 gtggcggaag acggcattgc gggagttaac gcgtttaccg tcgacgagaa tcagggtgta
    54961 gctgctgtcc agaccacgaa tactaacgcc cttacggtta tccccttcgt tcgtcagttg
    55021 tacgccaggc acttctttga ggacatcctt cagattctgt accggttttc gctgcaggtc
    55081 ttcctgggta atgacgctga tactggcggg tgcatctttg aggttttgtt caacggaaga
    55141 tgcagtgaca accatcgttt cgccatcatc atcgaccgct aacacaggcc atgcacaaga
    55201 aatagcggac aaacacagcc cgacccgtac gaaagggttc aacctaaaca ttccatatct
    55261 ccatgaggta actacgaaaa taaaatgggt tatcgctcac atcttcttca cgtccccttg
    55321 cgtacggcag catcgcggtg gacttatttg tatgcaggtg atcatcccag aaagccggaa
    55381 cagcctctga tttgtcagcc ttttttgatt gcggcgtaac gataatgcaa acgataacaa
    55441 ttatcaatcc aaatgttaaa ttttatatcc gcatgatttg tcagggaaat ggaattgaaa
    55501 aagccctctc ggttgagagg gcttagcaag gaagggagga aacttatttc ttatcgttct
    55561 gcgggaactt catttcgctg tagcgtacga agtgagttcc tttaatcagc ttgtagccga
    55621 accaaataat caggaacagc gggataccaa tatacgttgc cgctacgccg ccccagtcaa
    55681 tggtatcttt caggaacgct tcgtagttct ggcccaaagt gataatcaga cacagaatga
    55741 atgcgaagat cggccccagt gggaagaaac ctgaacggta cggcagatcg ttaatgtcgt
    55801 gtccctgcaa tacgtaaccg cgacggaagc gatagtggct aatggcaatc cccagccagg
    55861 cgataaaacc cgtcatcccg gaggtgttca gcagccacag gtataccgtc tggttgccaa
    55921 acatggaggt caggaagcac agaccggcaa tcaccgtcgt cgcatacagc gcattacgcg
    55981 gcacgccacc acgcgacagt ttagcgaaaa tgcgcggcgc tttaccgtca cacgccaggg
    56041 tgtacagcat acgagtagac gcatacatac cggagttacc cgctgacagc accgccgtca
    56101 gaataactgc gttcatcacc gccgccgcag agagcagacc cgcgtgctgg aacaccaggg
    56161 tgaacggact aacgctgatg tctttaacat cgttacgcag caggctcgga tcggtgtacg
    56221 gaataatcag gctgataatc aggatcgcga acacatagaa caacaggatt cgccagaaca
    56281 cctgacgtac cgcgcgtgga atgtttttcg ccggatcttc ggactcgcct gcagcaatac
    56341 cgatcagctc ggttccctgg aaagagaagc cgacaatcat agctacgccg atcatcgccg
    56401 caaaaccacc agcaaacggc gcttcgccga ttgtccagtt gctccagccc gcaggctgcg
    56461 cgcctttgaa gataccgata atcatcagca cgccaacgat gataaagaca ataactgtcg
    56521 tgactttgat cagtgagaac cagtattccg cttcaccaaa gccacgaact gagatgtagt
    56581 tcagcaggaa gataacgccg aggaacaacg cactccagat ccagcccggt gtatccggga
    56641 accaccagct catgaccagc tgagctgcaa ccaggtcaac ggcgatagtc accgcccagt
    56701 tgtaccagta gttccagccc agcgcgaagc caaagccttc ttcaacatag ttctgaccgt
    56761 aagtggcaaa cgaaccggaa accggcatat atgcagccag ttcaccgaga ctggtcatca
    56821 ggaagtaaac catcaggcca atcagcatat acgagagcaa tgccccgccc ggacctgcct
    56881 gagaaatcgt tgcgccagag gcaacaaaaa gacctgtacc gatggaaccg ccaatggcaa
    56941 tcatcgtcag gtgacgcgcc tttaattcac ggcgtaagcc cggcgcttct gtggttttag
    57001 tttcggaaac catacaaaaa tgctatccat cttaaaaatg aggcgcgatt gtagcagacg
    57061 attggcaatc cttccggcag aaatacacgg ttataagaca ccttcatgat cgcccaggga
    57121 ttataagtaa agcagccaat ctcttttctg gcgaatgcat gctgagggcg ctgcgtcacg
    57181 aaatcgacac agcaccagca tgttcttgta cagcaactta acgcggtaca ttcgcgggat
    57241 cgcaatagtc cagaaagcgc cgtagcgcgt tggaaaggtg tttttgccga tgatgtatac
    57301 gccacaacgt acgcatcagg cgcgggagag ggaccgcaac ttcacttaat gtgcctgcct
    57361 gcaattgatc tttaatcacc cgtcgcgaca ggcaactaat tcccaaccca tgacgcaccg
    57421 catgtttgat tgcctcggag ttacctaatt ccatcgccat ctcaaacttc ggtaaatgtg
    57481 acagcaacag ataatcgaca atctcccgcg tgccggaacc gcgttcacgc aggatccacg
    57541 gcgcagcggc cagctgttct aaggtgaccg gaccacgagc caacggcgaa gtcggcgcgg
    57601 cgaaaaccac cagctcgtct tccagccacg gttcagaaat gatttcagtg ctgtggcacg
    57661 gtccttcaat aaagccaata tcaacgcgga aatccagcac cgcttgcatc acgtcctggc
    57721 tgttcccaac gctaagttca atcggcaact gcggataatc atggcgataa cgggcgataa
    57781 ctgcaggcag aatgtagtta ccgatggtac tactggcata gatacgaatc gcgccgttgt
    57841 cttcgcgaaa cagttgttcg atttcaaccg cctgttcaag caatgccaac gcacgcggat
    57901 agagcagccg cccgtgttca ttaacaacca gtcttttccc cacgcgatca aacagttgca
    57961 cgccaagctg cccttccagg tcggtcaagg ctgcgctcac tgctgattgc gacaacgcca
    58021 gcatcaccga cgcctgggtg gttgatccac ttttcaatac ttctgcaaaa acttccaact
    58081 gccggagggt gatgtgcata gtcgcttacc acttataaag attaattata aatatataat
    58141 caattttatt tttaaaccag ttagtcgtaa ccttatgccc ggttaaagga gagggttatg
    58201 acgaatatca ccttacagaa acaacatcgt acactgtggc attttattcc ggggttagcc
    58261 ctgagtgcag ttatcaccgg ggtcgccctg tggggtggtt ccattcccgc ggttgcgggt
    58321 gccgggttta gtgccctcac cctcgcaatc ttgttgggga tggttttagg caacaccatc
    58381 tatccgcaca tctggaaaag ctgtgacggt ggtgtgctgt ttgccaagca atatttatta
    58441 cgtctgggta tcattcttta tggcttccgt ctgacgttct cgcaaattgc cgatgtcggt
    58501 atcagtggga tcatcattga cgtgttgacg ctgtccagta ccttcctgct ggcttgcttc
    58561 ctggggcaga aagtgtttgg tctggataag cacaccagct ggttgatcgg tgccggtagc
    58621 agtatctgtg gtgctgccgc ggtactggcg actgagccgg tagtgaaagc ggaagccagt
    58681 aaagtaaccg tggctgttgc aaccgttgtt atcttcggga ccgtcgctat tttcctctac
    58741 ccggcgatat atccgctgat gtctcaatgg tttagtccgg aaactttcgg tatctacatt
    58801 ggttctactg tgcacgaagt ggcgcaggtg gtggcggcag gtcatgccat cagcccggat
    58861 gcggaaaacg cagcagttat ttccaaaatg ctgcgcgtga tgatgctggc tccgttcctc
    58921 atcctgctgg cggcgcgtgt taaacagctg tctggggcga acagcggcga gaaaagcaaa
    58981 atcactattc cgtggtttgc catcttgttc atcgtagttg ccatctttaa ctcgttccac
    59041 ctgttaccgc agagcgtggt gaacatgctg gtaacgctgg ataccttcct gctggcaatg
    59101 gcgatggcgg cgctgggtct gaccactcac gtcagcgcgc tgaaaaaagc cggggcgaaa
    59161 cctctgctga tggcactggt actgtttgcc tggctgattg ttggtggtgg tgcgattaac
    59221 tatgtgattc aaagcgtcat cgcataaacc actacatctt gctcctgtta acccgctatc
    59281 attaccgttt tcctccagcg ggtttaacag gagtcctcgc atgaaataca ttggagcgca
    59341 cgttagtgct gctggcggtc tggcaaatgc cgcaattcgc gccgccgaaa tcgacgcaac
    59401 cgcgtttgcc ttgttcacca aaaaccaacg tcagtggcgt gccgcaccgc tcacgacgca
    59461 aaccatcgat gaattcaaag cggcctgtga aaaatatcac tacacatcgg cgcaaattct
    59521 tccccacgac agctatctga ttaacctcgg acatccggtc actgaagctc tggaaaaatc
    59581 gcgcgatgcc tttatagatg aaatgcagcg ttgcgaacaa ctggggcttt ctttgctcaa
    59641 cttccaccct ggcagccatc tgatgcagat ttcagaagag gattgccttg cgcgtattgc
    59701 cgaatccatc aacattgcgc tggataaaac tcaaggtgta acagcggtga ttgaaaacac
    59761 cgccggtcag ggcagtaact tagggtttaa attcgaacat ctcgcggcga ttatcgacgg
    59821 cgtggaagat aaatcccgtg tcggcgtctg cattgatacc tgccatgctt tcgctgccgg
    59881 gtatgatttg cgtactccag ccgaatgcga aaaaacgttc gctgattttg cccgtattgt
    59941 tggctttaag tatctgcgcg ggatgcacct taacgatgcg aaaagcacct ttggcagccg
    60001 cgttgaccgc catcatagcc tcggtgaagg caatatcggt catgatgcgt tccgctggat
    60061 catgcaggac gaccgtttcg acggcattcc gctgatcctt gaaaccatca acccggatat
    60121 ctgggcagaa gagatcgcct ggctgaaagc gcaacaaact gaaaaagcgg tggcctgatc
    60181 gatgaacgac agggaaaagc agattcttaa aattttacgg cgtaacccgc tgattcaaca
    60241 gaatgaaatc gccgatattc tgcaaatcag ccgctcatat tatggatttg atgcgcaagg
    60301 gtttgattaa aggcaaagga tatatcctta ccgagcagga ttactgtgtg gttgtgggtg
    60361 caatcaatat ggatattcgc ggaatggcgg atatccgtta cccgcaagca gcctctcatc
    60421 caggttctgt tcactgttcc gcaggcggcg tcggacgtaa tatcgcgcat aacctggctc
    60481 tgcttggtcg ggatgtgcat ttaatatcag ctattggcaa cgatttttac ggcgagacat
    60541 tactggaaga aacgcgccgc gcgggcgtga acgtttcaaa ctgcattcgt ctgcatggac
    60601 acagcactgc aacttatctc gctatcgcta ataagcagga agaaactatc ctggcaatca
    60661 atgacacaca tattctgcaa cagctaacgc ctcaactgct caatacttca cgggacttga
    60721 ttcgccatgc tggtgtggtg ctggctgatt gtaatctcac acctgaagcc ctggagtggg
    60781 tctttaccat tgctgatgaa attccgatgt ttgtcgatac cgtttccgaa tttaaagcga
    60841 acaaagtaaa aaactggtac agccggatcc acactctgaa accaacacaa aatgaactgg
    60901 aaattttgtg ggggcaaccg attaaagacg acaacgatcg catcagagct gtaaattcgc
    60961 tgcatcaaca aggtgttaaa cgcatttttg tttatctgaa agatgaatct gtattttgca
    61021 gcgataagga tggagaacaa tttttgctga ccgcgccagc gcatacgaca gtagacagtt
    61081 ttggtgctga cgatggtttt atggcgggcc tggtatatag ctttcttgaa ggatacagtt
    61141 tccgcgacag cgcccgtttt gcggtagcct gcgcggcaat ttcgcgcgcc agcggcagct
    61201 taaacaaccc taccctgtct gccgataacg cgctttcatt agtgccaatg gtgtaacaat
    61261 gttgccggat tcgacgcttg accgcatctt atccggctca taaattgcgc actacgctaa
    61321 accaataaag aatcctgcaa tagtagcact catcaggtta gaaagtgtcg ccgccgccag
    61381 cgcgcgtaaa ccaagttggg cgatttccgg cgcacggtgt ggcgcaaccg cagaaaacgc
    61441 ccccaccacc accccgatag aaccaaagtt agcgaaaccg cacaacgcga aagaaataat
    61501 cgccacggtt ttagcatcca gagtgccacc cgtttgcaga tatggcgaga aattgagata
    61561 agcgacaaat tcattgatcg ccagcttctg cccaatcaaa ctcccggcaa gatttgcatc
    61621 actccagtca acccccatca cccacgccaa tggggccaac aggtaaccta aaatggactc
    61681 cagcgaggca tgtgcaaaac caaaccagcc gccaacgccg ccgataatac cattaattaa
    61741 cgcaatgatg gcgacaaatg ccataacaac tgtcgctaca ccggcggcga ttttcagccc
    61801 ggtcattgcc cctgtcgcag cggcttcaat aatgcttttt ggcggtgttt cggtgaaaga
    61861 gaggttatta aaggaaacct gcgaagattc cgtagccggg cttaacaggc gggcaaacaa
    61921 gatcccgcct gggatcgcca ttaacgatgc cgccagcaaa tattccacag gtacgcccag
    61981 ggcggcgtaa ccaatcattg tcgaaccagc aatcgaggcc atgccactac aaatcgctgt
    62041 aaataattca ttgcgattca gacgatcgat aaagggtttg acgattgccg gaatttcgtt
    62101 ttgcccgagg aaaatggtag tgaccgcgac gaatgactag atcttgctga tatttaacgc
    62161 tttctggaag ataccgccga gaatgcgaat taaaatcccc atcacaccga tgtagtagag
    62221 aatactcacc agtgctgtaa cgaagataat tgccggtaat actctgaaac caaagataaa
    62281 tcctgcgcca tcaaacagcg tgtccatttt cggcccgacc agcgaaccga agataaatgc
    62341 gctacccgcg tcgctgtacg ccatcacttt atgcacgcca aaagcgactt tttcagcgac
    62401 ccaacgccct ggcggtaacc aaagcataat gccgccaatc acaacctgta acactaacgc
    62461 cgcgccaacg gtacgcaggc tgatcttctt cttgtttact gacagcaaaa atgcaattgc
    62521 cagcaatacc accattccca gaacacttct catgacatcc atagtgatta tctcttcatg
    62581 tcatattggg cgacggcttt ctgcccatca gaaatagccg ggcctgcatc ttgcgctatc
    62641 ggcccggctt gttatcaacg cttaatgggt tttgatgtag ccgcgcacgc actcttcgac
    62701 taagccccag aaccagtcgg tatcaatagt gatgccgact ttggtattgg cgggcttgcc
    62761 aagaacgccc agctcgtcgc agacggtgcg cccatagcaa gggccactgt tgacgtccac
    62821 ttcgacgtac atctcctggg ttttaatgcc atcagggtta atcagataac cgatgcaggt
    62881 ggcgtcgtgc accgggccgc cagccaggcc gtagttttcg aactgcgttt tgagagtgaa
    62941 gttcatgatg tcgctgaaca gctctccggc agggccgcct gccctttcca tccgagcaat
    63001 cacgtccggg gtgcaaacgg tctggttggt gagatcgagg cccatcatca ctaatggaac
    63061 gccagaggtg aacaccacgc gggctgcttc cgggtcagca aagatgttga actcggcaga
    63121 tggcgtgaag ttgcctgtac cgtaagcgcc gcccattagc acaatttcac ggattttggg
    63181 caggatcgcg ggttgcatac gcattgccac cgcgatattt gaaagcggac caaccggcac
    63241 cagagtgata tcgccatcgc ttgccatcag ggtatcaatg atgtatttca ccgcgtgggt
    63301 actttctgcc tggcgggtta gcggctcgaa taccgggcca tccagtccgg tttcaccgtg
    63361 aatattatcg gcaacgattt gttgacgcat aatgggttgc ggcatccccg cataaaccgg
    63421 aacattaatc tccagtttct ggcaaacatt caggccatta attaatgttt tatcaagcgt
    63481 ctgattaccc gctacaatag tgatgcctaa taaatctatt gccggatgtt tcgctgccat
    63541 catcattgca atagcatcat catgacccgg atcacaatcc agaataattt ttctcttttc
    63601 cattgtttat ttcctctgtt tccagttgcg ttattttttc tacagcaaag aaaattcgac
    63661 cgggccgatg attgaatctt accaaccgcg tacgtatgct aaatatgaga aatctcatag
    63721 cggataaaca tcgtgaaaga aatccacaat aatgatctta agcagcaatt gatgagtgaa
    63781 tccgcgttta aggattgctt tttaacggat gtttcagccg atacgcggct gtttcatttt
    63841 ttagcgcgtg actacattgt gcaggaaggg caacagccgt cctggctgtt ttacctgacg
    63901 cgaggccgcg ccaggcttta cgccacgcta gctaatggtc gcgtgtcgct gatcgatttc
    63961 tttgccgccc cctgttttat tggcgagatt gagttaatcg acaaagacca tgaaccgcgt
    64021 gcggtgcagg ctattgaaga gtgttggtgc cttgcgctcc ctatgaaaca ttaccgcccg
    64081 cggttattaa acgacacgct atttttacga aaactctgcg tcaccttaag tcataaaaat
    64141 tatcgtaata ttgtttcttt aactcagaat caatcatttc cgttagtgaa tcgcctggca
    64201 gcatttatat tactctcgca ggaaggtgat ctttatcacg aaaagcatac gcaagcggca
    64261 gagtatttag gcgtttctta tcgacatctt ttatatgttc tcgcgcaatt cattcacgac
    64321 ggtttattaa ttaaaagcaa gaaagggtat ctaattaaaa acagaaagca gttgtcagga
    64381 ctggcgctgg agatggaccc ggagaataaa ttctccggga tgatgcagta aaaattattt
    64441 gcaatagcgc gattgccgga tgcaacactt aataagcgcg tacatccggc aatgaaactc
    64501 aagctaaacc aataaagaac ccggcaatgg tcgcactcat caggttggaa agcgttgctg
    64561 ctgccagcgc ccgtaaacca agctgggcga tttccggcgc gcgttttggc gaaatagccg
    64621 aaaatgcgcc aacgacaaca ccgatagaac caaagttagc aaaaccacaa agcgcaaagg
    64681 agataatcgc aatggttttc acttccagcg tgccgcccgt ttgcaggtat ggggagaaac
    64741 tcaggtaagc gacgaattcg ttaatcgcca gtttctgccc aatcaggcta cccgcaagat
    64801 tggcatcact ccagtccaca cccatgatcc acgccagcgg tgccagcaca tagccaaaaa
    64861 tactttccag agaggcattg gcgaaaccaa accagccacc aattccgccg ataatgccgt
    64921 tgatcagcgc aataattgcg acaaacgcca ttaccaccgt cgctacacca gcggcgattt
    64981 tcagcccggt catcgcaccg ctcgccgccg cttcgataaa gctttttggc ggcgtttcgc
    65041 tgaacgacag attttcaaat gtgacctgtg aaggctcggt ggccgggcta agaatacgtg
    65101 caaacaaaat accacctggg atcgccatca gcgatgccgc taacaggtag tcaattggta
    65161 cgcccattcc ggcataacca atcatcatcg aaccagcaat ggacgccatc ccgctacaaa
    65221 ttgcggtaaa caactcgttg cgattcatgc gatcgataaa cggtttaacg atcgccggga
    65281 tctcattttg cccgaggaaa atagttgtga ctgcgacaaa agattcgatt ttgctgatgt
    65341 taagagcttt ctggaaaatg ctgccaagga tgcgaatcag cagccccatc acgccaatgt
    65401 agtacagcag actgatgagc gcagtaacga aaataatcgc cggaagtacg cgaaaggcga
    65461 agataaaacc cgcaccgtca aacaggacat ccattttcgg cccaaccagc gaaccaaaaa
    65521 tgaaggcgct accggcatca ctgtaagaca tcactttatg aacgcctaat gccgcctgtt
    65581 ctactgccca ctttcccggt gggaagtaga gcatgatgcc accaatagcg atttgcagca
    65641 gcagtgcggc tccaaccgtg cgcaaactga tgctcttttt attcactgac aacagaaatg
    65701 ctattgccag taacaccacc atccccacaa cgcttctcat tatatccata atgattttcc
    65761 cttcatgcca gtaaacccgg cgtcagcgcc aggttttggt atgcttgatg agtacgggcg
    65821 acggctttct gcccgtcaga aattaacccg cgagacgctg gtattctttg gcaatttcac
    65881 tcgccagaat ggcgttgttg aacaccagct ggatattgga tttcaggctg tcaccgccgg
    65941 tcagctcagc aacgcgcgcc agcaggaacg gtgtactttc tttaccaata acgccctgct
    66001 cttcagcttc ggcgacggct tgatctatgg ctgcattgat agattcctcc ggcatcgcaa
    66061 actgttccgg gatcggattc gccactacca ggccgccgtt caaaccggtt tgccatttca
    66121 ctgccattgc acgagcgatt tccgtggcac tatcaaggcg aatactgact tcaaatgggc
    66181 tggtacggca gaaaaacgca ggcagcgctt ttgtctgata gccaattaac ggcacaccga
    66241 aggtttctaa atattctgtt gttaatccta aatcgagaat agatttcgcc ccggcacaaa
    66301 caacggtgac attagtattt gccagttctt gcaaatcggc agaaatatcg aaggtatgtt
    66361 ccgccccgcg atgcacacca ccaattcccc cggtggcaaa tactttaatt ccggcaagcg
    66421 ccgcaataat catcgttgaa gccacagtgg ttgcgccatt ttttccggcg gcaacaacaa
    66481 aaggtaaatc gcgacgacta actttggtca cgttatgccc ttcacgaccc agtaattcaa
    66541 tttcttcttt gcttaaaccc actttcatca cgccgccaat aatggcgata gtggcaggta
    66601 ctgcgccctg tttacgaata gtttcttcta cttcaattgc ggtctgggca ttttgtggga
    66661 acggcatccc gtgagaaata atggtcgatt ccagcgccac aaccggtttt ttgtttttta
    66721 aagcgtcctg cacttccggg gaaatttgta ataattcagg ggaaattttt aattcagaca
    66781 ttctgcgttc tccactaacg atataacgtt ggcaatcgat aaatcggggt tattggtgta
    66841 ttcacaggag agcgccattg acgaacatcc ctgtgcgaaa cgaacagatt cggcaaacgg
    66901 cattccgtct acccaacacg aagcaagtcc cgccatcatg gcatcgcccg ctccggtaac
    66961 attaataaca ttggttttga tcggggcaga ccagccactt tcaccgctga tatcactgta
    67021 ataaacgcca tcgccgccca tgctcaatac cagtcggttc aggccatgtt gatggaacca
    67081 ggcagcaact tttgccacat ctccacgccc tgacagcgca atcccactca gtgtttccgc
    67141 ttcaaggcgg tttggcttca gagtgtggat ctgatttaga cggtcgcgca ctttgacaca
    67201 tttccacgct gaaaccggat cgacaaatac gggtacgttg gcagcattat ccagaatcca
    67261 tgccagtgcc tcttcactga tattacagtc cgcgacaatg acctttgccc tctgaataaa
    67321 ttcacggtgc tgtgcgagat attcagctgt aatagcgttg ctaatattca tgtcatttat
    67381 agcaaccagc atttcaccgg tattatcgag taatgataaa taactcgacg tattttctcc
    67441 cggcacaatc aggcatttat cgacataaac gccagattga ttggtttgcg ttagcagcga
    67501 ttgaccataa aaatcactac ctactgcgct cagtaaccag gctttgttac ccagcaacgc
    67561 caggttttgt gcaatatttc gccctactcc accaggtgta aattttattt tacctggatt
    67621 tgaatccgca taatttaatg attcatgtga atatccggcg acatcaatat tcgccgaacc
    67681 tataattacg acataatcct tttcgcgcat agcacgtcct ctggtaaaaa attaccaacc
    67741 ctgtcaatcc acaagaatat agatactgca aagacagttt aaacatgtgt tcatatttaa
    67801 acatgtgctc atggtatgca tgttttacgt aaagtaaatt gccgccagag aggcaaagcg
    67861 ggcagatttg agcaggctca caaaatgcga ttaaatgcca gaattacaaa agcgaggaat
    67921 ttgtgacggg atgcacagaa caggaacact aaaaaagggc agaaaagaat ctgcccttga
    67981 ggaaataacc ttagtgtttg atgaacgttt caggtttcta ctgaaagata aaaccattta
    68041 aattcataaa attacattca caaaccggtc attctctttg cgctcatcca atgatacagg
    68101 gcagtaaatt tctgccctgt aacacacctt ttattacgcg gctttcgcta ctgcgtccac
    68161 ttccggacgt ttcaggaagg cataggccaa acccgccacc agcgtaccgg caataattgc
    68221 taccaggtaa cccagtaccg gcgtaatagc gccagggatc agcagaacaa acagaccacc
    68281 gtgcggtgcc atcagtttcg caccaatcgc cattgagatt gcgccagtca gcgccccacc
    68341 aacgatacaa cacggcagca cacgcatcgg atcacgagca gcaaacggaa ttgcaccttc
    68401 cgaaatgaag cacagtccca ataccagagc ggctttgcca ccttcctgct gcgctttgtc
    68461 gaatttgcga cgcgccacca ttgttgccag acccattgcc agcggtggca ccatacctgc
    68521 cgccataatc gccgccatcg ggccataggt ttgagtactc agcagaccca caccgaatgc
    68581 gtacgctgct ttgtttaccg gaccgcccat gtcagtacac atcatgccac cgaggatcgc
    68641 ccccagcaga accgcattcg cagtccccat ggtctgcagc cagtgagtca acccttcgag
    68701 aatgccagca actggtttac cgatcaggta gatcatcgcc agaccgacca ccagactgga
    68761 aattagcgga atgatcagga tcggtttcag cgcctccata ctctgtggca gtttcagttg
    68821 cgtactgatt aacttcgcaa tgtaaccagc caggaagccc gcaataatac cgccaatgaa
    68881 gccagaaccg gtgctgaccg ccagcatacc gccaatcaga cccggcgtga gacccggacg
    68941 atcggcaatg gagaaggcaa tgtaacctgc cagtaccggc accatcagcg caaaggctga
    69001 accaccacca atctgcatca gcgccgctgc caacgtaccc ggctctttaa acgcttcgat
    69061 accaaaagca aaagaaagcg cgatacacag accacctgca acgaccatcg gcagcatgta
    69121 agagacgccc gtcagcaagt gacggtatgc gcctgcactc tctttcttac cttcagaggt
    69181 cgccgtttga gctttgcccg ccggttcata cggcgttgct tcagcaaccg ctttatccag
    69241 ttcctgcgcg gttttcttca gcgccagacc ggtagaggta cgatacatcg gtttaccagc
    69301 aaatttcgcc agatccactt cgatatctgc cgccacaatc accagatccg ctgcggctac
    69361 ttcttcgggg gtgattgcat tacccgcacc aacagaacca cgggtttcaa ctttcaccca
    69421 ccagccacgt tttttcgctt cggtttcaat ggcttcagcc gccataaagg tgtgtgctac
    69481 gccagtcggg caagcagtca ccgcaactac gcgtttcgga ccgctggcgg caaccggtgc
    69541 tgtcgcagcg accggcgcag tgtaaggttt cgcatggcct ttggcttcac tcaggaacag
    69601 ctcagggtgc gcaactgccc gggaaatatc acccagccag acatttttac cgttcagcgc
    69661 gctgtcattc gggatggaat caccgagaac aatcgccatt tctgcgtcgt tcggattgtc
    69721 gatgatttcc agttttgctt ttcgcgccgc cgcgcccagc agggtcttcg ccatataggc
    69781 gcgtgcctga ccgagattag cgtcaataat cagcagcgtt ttcattatgc ctctcctgct
    69841 gtcagttaaa aggttgtaag tcgacgcgcg ccatcattgc ggccaactgc ggacgatcgg
    69901 taatacccac attgctttga cttaccgcca gggctgcaac agcagtcgcc agacgcagtg
    69961 tgtgttcact ggattcacgc atcagcaagc cataaatcag gccaccaacc atagaatccc
    70021 ctgcgccaac ggtgcttacg acatcgactg acggtggttt ggcgatccat tcgccggagg
    70081 cattaaccca aagcgcgcct tcggcaccca gtgaaataac aacatgcgca ataccttgtt
    70141 cacgtagcgc atgtgcagct tcaatcacat ctttcatttc aggcagttta cggcctgccc
    70201 agatttccaa ctcgcggcgg ttaggtttca ccagccacgg tgccgctttc aaacctgcta
    70261 ctaacgcttc acggctacta tcaaagataa tgcaaggaca ctgactacgc aggcgagtca
    70321 tccagtcggt gaacgcttcc gggctgacgc ctgacggtaa gcttccgctg acacagacca
    70381 tatcgaactg accgagccag ctcagagaat cagtcacaaa gcgttcccag tcggcggggg
    70441 tgacttcaaa acccgagaag ttgaagtcgg tcacttcgcc gtctttttcc gtcagcttaa
    70501 cgttaattcg ggtgcgcccc tgtacaacct ggaaacggtt ggcaatgccc agctcgctga
    70561 acagttgctg aaaaccatcc tgattgtctt tacccaggaa gccgccaacg gtgacatcaa
    70621 ttcccaggtc ttttaatact ttggccacgt tgatgccttt acccgccgca tgcagaccgg
    70681 tggttttcac caggttcact tcgccgcgtt caatttccgg gcagaaacca acaaggtcat
    70741 aagccggatt aagggtgata gtagcaacac gtctgctcat tatgcgccct ccccaagacc
    70801 agcagcgata gcgtcgccga ttgctttcag cgcctgttca gcatctgcac cctgggcggt
    70861 aaagcgtagg cgatgacctt tcttaacgcc aagtgccaca actttcatca gactacgtcc
    70921 gtttgccggt ttgccggtgc catcaaggtt tgtcacggta atatcactgt taaattgttt
    70981 aatggtgttg accagcatgg tacctggacg agcatgcagg ccgtgttcat tgcgcaccac
    71041 aaactccgcg cttaacacgt cgtcggtcgg cgcatcatcg ctggtcagca gcgccagcaa
    71101 cgttgccgca tccgctttca gcaagcggtc agctttattg tcgagcaaca aatcagcgag
    71161 acgcttaaga accgcaatgg gctgatcgtc attcatcgcc acactcacca gcatggctgc
    71221 cgtttcgccg tccacatcaa aagcatttgc cgcacggctt accgcaatcg cgctacgcag
    71281 attgccttcg gcgctatcgc tcagccagat accctgtccg aggttcagcg gttgttcatt
    71341 gatggctttg gtgacgaaag tggcgtcaac tgccctcgcc tctttcagac gcgcagcgtt
    71401 cagcgcctga agagtcagca gatcgctggc ggcgatatcc agcgtcagca tttcgttgtc
    71461 gagcttcagc tgctcactct gcttttcgcc catcagcaat gcgcgaagtt cttctgctgt
    71521 tgttgctgac ttcagttgtt cagcaacgga atcatcgctc agtacgtggg tcagctggcg
    71581 tagcaggccc agatgttcat ccgagctggc agcaataccg atcgctacgt acgctacctg
    71641 accgtcaccc caggtgacgc cttccgggaa ctgaaatacc tgaacgccgg ttttcagcac
    71701 ctgatcgcgg gtgtcggtag tgccgtgtgg aatagcaata ccattgccga ggaacgttga
    71761 ggtttgctgt tcgcgcgcca gcatgccatt gacgtagcct tctgctacgt taccggcctg
    71821 caccagcgcc gcagcgagct ggcgaatcgc ctcttctttg tctccggcct tttcgcccgg
    71881 atggatgtcc tgtacggata actggaacat agttctcctc tcttgctgaa ttgaaacgat
    71941 tcagcttcta tgagaaaaaa agcgccaacc tggcttaggg ttaaagacaa gatcgcgctg
    72001 aaacgtttca agaaagcata atacttctgt ttcagcacgc aaggaaagcc gtaaagttat
    72061 gagcaaaagt ttgatgtgct gcacaatttt ttgtcatttt tcctaattgc tgatgggaaa
    72121 atccgttatc agcaatttca tttcagcagc gtcagcagcc aatcagcaac agtgaaatgg
    72181 cattttgatt attcgtggcg cttttgattt cgctgtctgt ttattttctg acaagcagcg
    72241 taaactccgc gtcttcctct tccagtgatc gaccagcatg cataactccc ccgcagtctc
    72301 cagcgcgaaa tcgtttgacc tgacctcgac ggcgttttta atcgttgcct ttctcaccgg
    72361 tattgcgggc gctctgcaaa ccccgacact cagtattttt cttaccaatg aagtacatgc
    72421 ccgtccggcg atggtgggat tcttctttac cggcagcgct gtcattggga ttctggtaag
    72481 tcagtttctc gccgggcgct ctgataagcg cggcgatcgc aaatcgctga ttgtcttttg
    72541 ctgcctgtta ggcgtgctgg cctgcaccct ttttgcctgg aatcgcaact actttgtttt
    72601 gctattcgtt ggcgtctttc ttagcagctt tggctcgacc gctaacccgc aaatgtttgc
    72661 ccttgcccgt gaacatgccg acaaaaccgg acgtgaggcg gtgatgttca gctctttttt
    72721 acgcgctcag gtttcactgg catgggtcat tggcccaccg ctggcttatg cgttagcgat
    72781 gggtttcagc tttacggtaa tgtatctgag cgcagcggta gcgtttattg tttgcggcgt
    72841 gatggtgtgg ctgtttttac cgtcgatgca aaaagagctt ccgctggcga ccggcacggt
    72901 tgaagcgccg cgccgtaacc gtcgcgatac gctactgctg tttgtcattt gtacattaat
    72961 gtggggctcg aacagcctgt acatcatcaa catgccgcta tttattatca acgaactgca
    73021 tcttcccgag aaactggccg gtgtgatgat ggggaccgcc gccgggctgg aaatcccgac
    73081 gatgttgatt gccggatatt tcgccaaacg tctgggtaag cgtttcttaa tgcgcgttgc
    73141 tgccgtgggt ggcgtctgtt tttacgcagg aatgctgatg gcgcattcac ctgtcattct
    73201 gttgggcttg cagctgctaa atgctatttt tattggcatt ctgggcggca tcgggatgct
    73261 ctattttcag gatctgatgc ccggtcaggc gggttcagcc accacgctct ataccaacac
    73321 ttcgcgcgtg ggatggatca tcgcaggatc agtggcgggc atcgtcgccg agatctggaa
    73381 ttatcacgct gtgttctggt ttgcgatggt gatgattatc gccactctgt tttgcttact
    73441 gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc aggtcatggc
    73501 ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac agactttcgg
    73561 tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa tgcagggcgt
    73621 attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag gggcagtgca
    73681 acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta accaccggat
    73741 tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata ccacttgccc
    73801 gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact acaggaattt
    73861 ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca acggcaaact
    73921 gctgctggtt aaggatattg atattcagtc gcccactgcc cgcggtgccg ctacgctgta
    73981 caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt tcaaaggtga
    74041 tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct atgtcgatgg
    74101 caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca ccaaagatca
    74161 gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc aggtgctgac
    74221 ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg aaatcgttga
    74281 aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg cgacattgag
    74341 cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa ttcgtatcca
    74401 tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg cagaaaaggg
    74461 atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg cgcttcctga
    74521 acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc atcgcattaa
    74581 caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt taatttatcg
    74641 ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc atcatgtaca
    74701 cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg gctttggcgc
    74761 ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc agggcggcga
    74821 ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc agctccgcgc
    74881 acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg tgataattgt
    74941 cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa ttattgagaa
    75001 attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag gctattgtat
    75061 tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg atctacaaac
    75121 ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac gccgccgtga
    75181 gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca atggtcatgt
    75241 ggtgaaaaac gcggtgctgg gaatggcaga aaaacgttcg ccagaactcg ccgggtggat
    75301 aaaagagcac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg ctgcaaccga
    75361 cgaatcactg gtggaaatca gccagcatct gggggtgaat gatccctgcg cgattagctg
    75421 cgaaccgttt atccagtggg tggtggaaga taacttcgtc gctgggcgtc ctgcctggga
    75481 agtcgcagat gtacaaatgg tgaatgatgt cctgccatgg gaagagatga aactgcggat
    75541 gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat tcgcccatat
    75601 cagtgattgc atgcaggatc gcgcatttcg ccatgccgcc agaacattaa tgctggatga
    75661 gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg ataagttaat
    75721 tgcacgtttt gctaatccgg cgctgaaaca taagacctgg caaatcgcga tggatggcag
    75781 ccagaaatta ccgcaacgca tgctggcagg tattcgcata catcaggggc gcgaaacgga
    75841 ctggtcgttg ctggcattag gcgttgcagg ctggatgcgt tacgtcagcg gcgttgatga
    75901 tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg aacttgttgc
    75961 gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag ttttcggtga
    76021 tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc aacaaatcgt
    76081 acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat ttctgcggtt
    76141 aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgctt tttttagcca
    76201 ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca ggaccaacct
    76261 catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt tagcccataa
    76321 agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg gaattgatgg
    76381 tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct gcatgtgctg
    76441 cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg gaaaaccaga
    76501 ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg atctgttaac
    76561 cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc tcgatccgcg
    76621 cttgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc tggctgccgc
    76681 agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc aagcgctaca
    76741 gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc atgggaaagt
    76801 tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg ccagcgccgc
    76861 gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc cagagcatca
    76921 acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct ggatattcga
    76981 cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg caccggtgga
    77041 acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc gtcagggcga
    77101 tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg agctgatttc
    77161 cagcagcgaa gctgactgga atgctttgca gagcgcgctg ttgaagcttc gtttagcgac
    77221 taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat gattaaaaat
    77281 ttgccgcaaa tagtgttgtt gaatattgtc ggcctcgcgc tgtttctttc ctggtatatt
    77341 cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt ctttaatcag
    77401 aaactggtcg aaagtaaggc ctttttgtgg ctggttgcat tgaccaacaa tcgcgctttc
    77461 gacggttgtt ctctgctggc gatgggaatg ttgatgctga gtttctggct gaaagaaaac
    77521 gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac tgcagtggta
    77581 ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac attgactttt
    77641 accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc ctcacgagat
    77701 agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat gtggcgttat
    77761 ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc atttcccaga
    77821 gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac cgtgatattg
    77881 atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac cttttttgac
    77941 aaatcactac cgggaaaaaa caaacatttc caaaacaaat aactcacagt aattaacatc
    78001 atcaggctta tttttatagt gaggataatc ctgatgatgc gcaccgtgct ttcatccatc
    78061 gcgcgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac tcaatcgtac
    78121 tttaattgac caatcccgct tattaacttt ctgtatcact ttttcttata aaaaatcatg
    78181 taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt gcctcggcgg
    78241 tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc cttaaggaat
    78301 tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta taacgatatt
    78361 tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc gattttgaga
    78421 tatatcttgc gcgggatccc cgcgattgca gtagcggttc tgctttctgc atgtagtgca
    78481 aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga aacatcatca
    78541 ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt aaaatcgcga
    78601 attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg cagcactaaa
    78661 aaaggtatcg attgttctgg ttttgtacaa cgtacattcc gtgagcaatt tggcttagaa
    78721 cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg cagtaatttg
    78781 cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt tggtatttat
    78841 atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat ttccagcatg
    78901 aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag ccgcagctaa
    78961 taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag cactgcttag
    79021 gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct tgatccaggc
    79081 tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat taacggaatc
    79141 atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat tgttgcaggc
    79201 gtgatgattg cgatactggt gagttgcctt caatttttag tggcctggca taagcacgaa
    79261 gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta ttttgccgac
    79321 ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca gcaggctaac
    79381 cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt gctggtgaaa
    79441 gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact caatgaattg
    79501 attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg cacgccgatg
    79561 gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa aaatagcggt
    79621 gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc acggcaggaa
    79681 gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt ttcttcacgt
    79741 ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat tgcgggcatt
    79801 cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg gtacgcattt
    79861 ttactgggcg gcatgagtgg aactgtcgtt ggcctgctct gctattacct gatgagcgta
    79921 cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt ttacgtggcg
    79981 tatcaaccgg tggtggatac acaagctttg cgagtaacgg gcctggaagt actgctacgc
    80041 tggcggcatc ctgtcgcggg agaaattccc ccggatgcct tcattaactt tgccgaatcg
    80101 caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg cgatgccgca
    80161 gaattagaaa aagtgctgcc agtaggcgtc aaatttggta ttaacattgc gccggaccat
    80221 ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct acccgcacac
    80281 catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca agaagccaca
    80341 caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga cttcggcacc
    80401 gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa aattgaccgt
    80461 ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga cgcggtgctg
    80521 acgctggcga aacgtctcaa tatgctgaca gttgctgaag gggtcgaaac gccggaacaa
    80581 gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat tagtcgcccg
    80641 ttaccgctgg acgattttgt ccgctggctg aagaaaccgt atacgccgca gtggtaaggt
    80701 gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta atgatgagta
    80761 aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc tctgttcacc
    80821 tttggtgtgc aggcgcaggc tatcaaggaa agctatgcct ttgccgtgct gggcgaaccc
    80881 cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc aaaaggtggg
    80941 cagataacgt tgtcagccct cggcaccttc gataatttca accgctatgc actgcgcggc
    81001 aacccgggcg cacgcaccga gcagctgtac gacacgctat ttacgacttc cgatgacgaa
    81061 ccaggcagtt attacccgct gattgctgaa agcgcacgct atgctgacga ttattcctgg
    81121 gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttcgcccat tactgcccgc
    81181 gatgtagagt ttacttttca aaaatttatg accgaaggcg tgccgcaatt tcgtctggtc
    81241 tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga gttagctaaa
    81301 cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga aaagtactgg
    81361 aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg tccgtaccgc
    81421 attacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga ttactgggca
    81481 gcaagcttac cggtaaaccg cggacgctgg aatttcgaca ccattcgcta cgattattac
    81541 ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt gcgtatggaa
    81601 aacgacgcca aaaactgggc cacgcgttat accggtaaaa atttcgataa aaaatacatc
    81661 atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc gtttaatatc
    81721 caacgtccag tattcagcga tcgccgggtg cgggaagcaa tcactctcgc ctttgacttt
    81781 gaatggatga acaaggcgtt gttttacaat gcctggagtc gcacgaacag ttattttcag
    81841 aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct tctggcacca
    81901 atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc ggtatccaaa
    81961 ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa cgaagcgggc
    82021 tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccact cagctttgaa
    82081 ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca cagcctgcaa
    82141 cggctgggta tcaacatgga cattcgcaag gtggataact cgcaaatcac taaccgcatg
    82201 cgcagtcgcg actatgacat gatgccgcgc gtatggcggg cgatgccgtg gcccagttcc
    82261 gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc ccccggcgtg
    82321 caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg aaataaagaa
    82381 aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta ttacatgctg
    82441 ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc ccagccggcc
    82501 gtgcgcccca tctatagcct cggtatcgat acctggtggt atgacgtcaa taaagcggcc
    82561 aaactgccgt ccgccagcaa acagggagag tagatgggcg cttacctgat tcgccgtctg
    82621 ttgctggtga tcccaacatt atgggcgatt atcaccatca actttttcat cgtgcaaatt
    82681 gcgcctggcg gtccggtcga ccaggccatc gccgccattg agtttggtaa tgccggagta
    82741 ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg tgtcggcaat
    82801 atcagcgaca gtaagtaccg tggcggacgc ggattagatc cagaagtgat cgctgagatc
    82861 actcatcgct acggttttga taagccgatc cacgaacgtt acttcaaaat gctctgggac
    82921 tacatccgct ttgattttgg cgatagcctg tttcgcagcg cctcggtgct gacgctgatt
    82981 aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat tatctatctg
    83041 gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagccgctt tgacgtctgg
    83101 agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc catcctgctg
    83161 attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg cctggtttcc
    83221 gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg gcatatcacg
    83281 ctgccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct gacaaaaaac
    83341 tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa aggggtaagt
    83401 gaaaaaaata ttctctggaa acatgtgttc cgcaacgcca tgctgctggt gattgccggt
    83461 tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga ggtgatgttt
    83521 tcactcaatg gtctgggctt actgggctac gaagcgaccg tctcgcgcga ttatcctgta
    83581 atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat tgtcagtgat
    83641 atcagctata cgctggtcga tccgcgtata gattttgagg gacgttaatg tcgcgactca
    83701 gccccgtcaa tcaggcccgt tgggcgcgtt ttcgccataa ccgtcgcggc tactggtcgt
    83761 tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc gccaacgata
    83821 aaccgttgct ggtacgttat gacggcagtt ggtatttccc gttgttgaaa aactacagcg
    83881 aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg tggctgaaac
    83941 aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt gctaccagta
    84001 tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac tggctgggaa
    84061 cggatgccaa cggcggcgat gtgctggcac gtattctcta tggcacgcgg atctcggttc
    84121 tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg ggggcgctac
    84181 aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa gtatggtcgg
    84241 ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct aacttctggt
    84301 ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg gtgcgggcgg
    84361 agtttttacg cacccgtaat ttcgactaca ttcgtgcggc gcaggcgctt ggcgtcagcg
    84421 atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc ctcacctttt
    84481 taccgtttat tttatgtagt tcgataacca ccctgacctc actcgatttc ctcggcttcg
    84541 gtctgccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa aataaccttc
    84601 aggctccgtg gcttgggatc accgccttct tgtcggtggc gatattattg tctttgctga
    84661 tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg tagcatgacg
    84721 caaactctgt tagcgattga agatttgtcg gtgggttttc gccatcagca aaccgtacgt
    84781 acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc gctggtgggt
    84841 gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct cccttccccg
    84901 ccggttgaat atctctccgg cgatattcgt tttcgtggcg aatcgctgct tcatgccagc
    84961 gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca agagccgatg
    85021 gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct ttcactccac
    85081 cgcgggatgc gtcgggaagc ggctcgaggc gaaattctta actgccttga tcgcgttggt
    85141 atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg cggcgaacgc
    85201 cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat tgccgatgaa
    85261 ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt gcgcgaactg
    85321 caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat tgtcagaaaa
    85381 ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa taacgccgct
    85441 acgctatttg cctcccccac tcatccttac acacaaaagc tactcaacag tgaaccgtca
    85501 ggcgacccgg tgccgttgcc agaacctgcc tcaacgttgc tggatgttga acagcttcag
    85561 gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa tgtggtggtg
    85621 aaaaacatca gttttacgct acgagcgggt gaaacactgg gtttagtggg cgagtccggt
    85681 tccgggaaaa gtacgacggg actggcgctg ctgcgactga ttaattctca gggcagcatc
    85741 atctttgacg gtcagccact gcaaaattta aatcgccgcc agctgttacc tattcgtcat
    85801 cgcattcagg tggtatttca ggatccaaac tcctcgctca acccacgact caacgttttg
    85861 cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc acaacgcgaa
    85921 caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg ccaccgttat
    85981 ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc attaattctt
    86041 aagtcctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac ggtacaggcg
    86101 caaatattga cgctattgaa atcattgcaa caaaagcatc aactggccta tttgtttatc
    86161 agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact gcgacaaggg
    86221 gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca ggagtatacg
    86281 cgtcagctac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa agggttcggc
    86341 aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct ggcgatttac
    86401 aaacaggcac ggctcacctt cacactccag caccgaacat ggaacttcga tgtcgcttaa
    86461 cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc taagcaattt
    86521 aagagccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt caaccttcga
    86581 acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga ttgagagttg
    86641 taatgaagtc acttattttc cccggaagca cattcacttc acaatttgtt tacatcaatt
    86701 ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc ctcgcctgcg
    86761 tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt gcggggctat
    86821 atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg actttttaca
    86881 taaattgatt ttacataaaa taaacatata tcgggggaat gatagatttg tggttgacat
    86941 gcatcaacaa aggaagcctt ttagcttcct cgttgtgcaa tagatcaccg ttttttcggt
    87001 cgactggcgt acagacagaa gagaatggag ctgcttgcgc agaatgcaat tgaccaaatc
    87061 atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat tgcccctatg
    87121 ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg aaactcatca
    87181 agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa cgccgcaacg
    87241 ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat ccacgctgcc
    87301 ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat gcggcgaacg
    87361 aagcggctgt taaaaatggt catcacgaac aggaaaacaa tgtttagtgc aaagtaataa
    87421 ccaaagtttt ccggcgcgac gtggttaatt tcaatgtaga caaacggtcc ggcgcttaag
    87481 aacgagaaca tcccggcaaa gctgaaacca ctggcaagca tataactcag gacacgctta
    87541 tgacggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaaagg ctggcgacgc
    87601 tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag aatcgccgct
    87661 aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca accgccgaca
    87721 atcggtgcca tcagcggtgc aatagtggtc accagcatga caaacgacat catccgcgag
    87781 aactcttctt tcgggtaaat atcgcgcatc agggcgttaa tgaccacgct ggccgcagcc
    87841 gccgccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtgtt tgccaacgca
    87901 cacgccaccg cggcggcggc aaacaccagc gtaccgccga gcaccaccgg cttacgcccg
    87961 aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag aatataagta
    88021 ctgagggtca tctgcgtact gcccgcaggt acgccaaact gcgctgaaat taccggtagc
    88081 gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc aaggataaaa
    88141 acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa gtcatggaaa
    88201 agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc gggggctaaa
    88261 tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac gtggttaccc
    88321 acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt cagacgaacc
    88381 tgcgttgggg taatcacttc cagcaccgca ggcttagtga gatctttttc gttatgcagc
    88441 tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc cagtgtcacc
    88501 agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca ctgaccatca
    88561 tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc gtgcagtttc
    88621 caggctaccg gttcatccag aaaatagagc accgttgggt gatcagggtc gtccgtggag
    88681 caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccatgttg ctgcgcgagc
    88741 gggttgccat cgtaagcgac atcatgttca ggaagcagtt tgaacgctgc attacgaaca
    88801 atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat agcacggcta
    88861 acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt gcctgtaaaa
    88921 acggaggtcg ggcattgacc cgaaaatctg aactgttgtc tgcccagtat agcggtctaa
    88981 taacatccct caagggaaaa agatccatgg catactatta gcagaataat ctacctacgc
    89041 gagaccatga tttttacact ccgcccatat cagcaagaag ccgtggatgc cacgctcaac
    89101 cattttcgtc gtcataaaac ccctgccgtt atcgtgctgc ccaccggcgc aggtaaaagc
    89161 ctggtgattg ccgaactggc acggctggcg cgtggtcgtg ttctggtgct ggcacacgtc
    89221 aaagaactgg tggcgcaaaa ccatgcaaag tatcaggcgc tggggctgga agccgatatt
    89281 tttgccgctg ggttaaagcg caaagagagc cacggtaaag tggtatttgg cagcgtgcag
    89341 tcggtcgctc gtaatcttga tgcctttcag ggtgaatttt cgctgttgat tgtcgatgaa
    89401 tgccaccgta ttggtgacga tgaagagagc cagtatcagc aaatcctcac tcacctgact
    89461 aaagtgaatc cccacttacg cctgctgggg ctgactgcca cgccttttcg attgggcaaa
    89521 ggctggatct accagttcca ttatcacggc atggtacgcg gcgatgagaa agcccttttc
    89581 cgtgactgca tttatgagct gccgctgcgt tatatgatta aacacggcta tctgacgccg
    89641 ccagaacgac tggatatgcc agtagtgcaa tacgatttca gccgcttgca ggcacagagt
    89701 aacgggctgt tcagcgaagc tgatctcaac cgtgagctga aaaaacagca acgcattacg
    89761 ccacatatca tcagccagat catggagttc gccgaaaagc gcaaaggggt aatgattttc
    89821 gccgccaccg ttgaacacgc aaaagagatt gtgggattac tgcctgccga agatgctgca
    89881 ctgattactg gcgacacccc cggcgctgag cgcgatgtgt taattgaaga ttttaaagcc
    89941 cagcgttttc gctatctggt caacgtcgca gtactgacca ccggatttga cgccccgcac
    90001 gtcgatctta tcgccattct gcgccctacc gaatcagtga gtctttacca acaaattgtc
    90061 gggcgcggtc tgcgtctcgc tccgggcaag actgattgtt taattcttga ttatgcgggt
    90121 aatcctcacg atctctacgc gccggaagtt ggtacaccga aaggcaaaag tgacaacgtt
    90181 ccggtacagg ttttctgccc tgcctgcggt tttgccaaca ccttttgggg gaaaacgacc
    90241 gccgacggga cattgattga acactttggt cgccgctgtc agggatggtt tgaagatgac
    90301 gacggtcatc gcgaacagtg tgacttccgt ttccgtttta aaaattgccc gcaatgtaac
    90361 gcagaaaatg atattgccgc tcgccgctgc cgcgaatgtg acacggtact ggttgatccg
    90421 gatgatatgt taaaagcggc gctacgactg aaagatgcgc tggtattacg ctgtagcggc
    90481 atgtctttgc agcatgggca cgacgagaaa ggcgaatggt tgaaaatcac ctattacgat
    90541 gaagacggcg cggatgtgag tgagcgtttc cgtctgcaaa cgcccgccca gcgaactgcc
    90601 ttcgagcagc tttttatccg cccgcatacg cgcacaccga gcatccctct gcgctggatc
    90661 accgccgccg atatcctcgc ccagcaagcc ttactgcgac atccggattt tgtcgtcgcc
    90721 cgcatgaaag gtcagtactg gcaggtgcgt gaaaaagtgt tcgattacga aggtcgtttt
    90781 cgtcgggcgc acgaattacg cggttaatgg tagttttgat tgatgtataa ggcgattgag
    90841 tatagaatct cgcccgcttt tgcatacgca aagcagatca cttacctgtt gctgggtcgc
    90901 ctgtagcagg attaatttaa gagagaaaga aatgtttact atcaacgcag aagtacgtaa
    90961 agagcagggt aagggtgcga gccgccgcct gcgtgccgct aacaagttcc cggcaatcat
    91021 ctacggtggc aaagaagcac cgctggctat cgagctggat cacgacaaag tcatgaacat
    91081 gcaagctaaa gctgaattct acagcgaagt tctgaccatc gttgttgacg gtaaagaaat
    91141 caaagttaaa gctcaggacg tacagcgtca cccgtacaaa ccgaagctgc agcacatcga
    91201 cttcgttcgc gcttaattgc tgaataagtt gtaaaaaacc ccgctccggc ggggtttttt
    91261 gtatctgcag attatgcctg attacggtat tgctattttt tgcaggccag ataaggcgtt
    91321 cacgccgcat ctggcattca gaacacaaaa ccgattaatt gccgccagac gtccggcgct
    91381 gcaattggtc gcgcaaattc ggcggcatgc ctttaatggt caaggtatcg gttgctggat
    91441 cccagaagat gcgttcacct aacagcatcg catcaaagtt aatagtcaag ccaccgccgc
    91501 taccggcaaa cttcgtcaac tgacgcagtg tgctacggtc tgccgggaag ctctcttcca
    91561 gttcgtagcc tttttcagcc gcaaactccg tgaagctcac ttcgcttacg cctgccagct
    91621 ctttcgacag cgattccagt tcgatctctt cgcctgcctg cagctgctca ttacagtagc
    91681 tataaacctg ctggcgcacg ttctgtcgct ctgctttatc cagttgtgct tcggcagtga
    91741 aatcatcaac cgcttgcaac agcccacggt tttgcgcttt cgcgttcaat ccttcgctgg
    91801 ccccgaggaa atccataaag aaatccgcca ctttgcgccc tactcgccct ttcaggaaag
    91861 tgagatagcg ggtggactct ggattggttt cccattcggt taaatcgata cgcgcaacaa
    91921 tatccgcatg attgatatca agataatggg ttgggttgat gtcgagattt tcgttaacgc
    91981 gcatactgct caggttgctc agaactgcca ccagcagata ctccaccgcc agataacggt
    92041 agtggcaaaa cagcacgaat ccaccgtcag caaaaggata tttcgctaat tcgtcacgca
    92101 ggcgcccggt tgccgcgcgg ctaaatgcca gaaagtcctc ttcgccctga cgctgtaagc
    92161 gcagcgtctg cgccagttca ctctcttcgc taaacaagcc gtaagcttta tttttggcgc
    92221 tatagacccg atgcagttct gccaccatct caacgacggt ttctgtcggt tccagcaatg
    92281 aatcgcgcaa caccagctca aggttctgct catcacgctt gataagctgg tgcagggcaa
    92341 tctggttgat atccagactc atgataaact ctcctttaag accgggcggt attcaaccac
    92401 cgccggttaa gcgacgcaat aaaagatggc ggtcatttta cagaaggcag cgtaacgaga
    92461 atgatttaac gcataaaaaa gcagaaaaaa aaccgttgct acggtaatat gttgcccttt
    92521 catcaactaa ctgatttcga tttatgccac aaatttcccg ctacagtgat gaacaggttg
    92581 aacaattgct cgcagagctg ctcaacgtac tggaaaaaca taaggctccg accgaccttt
    92641 ccctgatggt gttaggcaac atggtcacta accttatcaa caccagcatt gccccggccc
    92701 aacgccaggc aattgccaac tcttttgccc gcgccttaca gtcctctatc aacgaagaca
    92761 aagcgcacta agggaaacag ataactggtt atggtaactc atcgtcagcg ctaccgtgaa
    92821 aaagtctccc agatggtcag ttgggggcac tggtttgcac tgttcaatat tctgctttcg
    92881 ctcgtcattg gcagccgtta cctgtttatc gccgactggc cgacaacgct tgctggtcgc
    92941 atttattcct acgtaagcat tatcggccat ttcagcttcc tggtgttcgc cacctacttg
    93001 ctgatcctct tcccgctgac ctttatcgtc ggctcccaga ggctgatgag gtttttgtcc
    93061 gtcattctgg caacggcggg aatgacgcta ttactgatcg atagcgaagt ctttactcgt
    93121 ttccatctcc atcttaatcc catcgtctgg caactggtta tcaacccaga cgaaaatgag
    93181 atggcgcgcg actggcagct gatgttcatc agcgtgccgg ttattttatt gcttgaactg
    93241 gtgtttgcga cgtggagctg gcaaaagctg cgcagcctga cgcgtcgtcg acgcttcgcg
    93301 cgcccgctgg ccgcattctt atttatcgcc tttatcgcct cgcatgtggt gtatatctgg
    93361 gccgatgcca acttctatcg cccgatcacc atgcagcgcg ctaacctgcc gctttcgtac
    93421 ccgatgacgg cgcgacgttt tcttgagaag catggtctgc ttgatgcgca ggagtatcaa
    93481 cgccgtctta ttgagcaagg taatccagac gccgtttccg tacagtatcc gttaagcgaa
    93541 ctgcgctatc gcgatatggg caccgggcag aatgtgctgt tgattactgt cgatggcctg
    93601 aactactcac gcttcgagaa gcagatgcct gcgctggcag gttttgctga gcaaaatatt
    93661 tcgttcacgc gccatatgag ctccggcaac actacagaca acggcatctt tggcctgttc
    93721 tatggcatct cgccgagcta tatggacggc attctgtcga cccgtacgcc tgcggcatta
    93781 attactgcgc ttaatcagca aggctatcag ctggggttat tctcgtcaga tggctttacc
    93841 agcccgctgt atcgccaggc attgttgtca gatttctcga tgccgagcgt acgcacccaa
    93901 tccgacgagc agaccgccac gcagtggatc aactggctgg gccgctacgc acaagaagat
    93961 aaccgctggt tctcgtgggt ctctttcaat ggcactaaca ttgacgacag caatcagcag
    94021 gcatttgcac ggaaatatag ccgggcggca ggcaatgtcg atgaccagat caaccgcgtg
    94081 ctcaatgcac tgcgtgattc tggcaaactg gacaatacgg ttgtgattat cactgccggt
    94141 cggggtattc cgctgagcga agacgaagaa acctttgact ggtcccacgg tcatctgcag
    94201 gtgccattag tgattcactg gccaggcacg ccggcgcagc gtattaatgc gctgactgat
    94261 cataccgatc tgatgacgac gctgatgcaa cgcctgctac atgtcagcac acctgccagc
    94321 gaatattcgc aaggtcagga tttgttcaac cctcaacgcc gtcattactg ggttactgca
    94381 gcggataacg atacgctggc aattaccacc ccgaaaaaga cgctggtgct gaacaataac
    94441 ggtaaatacc gcacttacaa cttacgtggt gaaagagtga aagatgaaaa accacagtta
    94501 agtttgttat tgcaagtgct gacagacgag aagcgtttta tcgctaactg attaattata
    94561 aatcagttag cgaaatatct tacttgcaat cggtgtggaa aacggtagta ttagcagcca
    94621 cgagtcggca cgtagcgcag cctggtagcg caccgtcatg gggtgtcggg ggtcggaggt
    94681 tcaaatcctc tcgtgccgac caaatcgccc accggatacc accggaaatc accgaataag
    94741 acagggatag cccgccaatt acttgattgg cgggtttttt tatggttttt agtcaccgaa
    94801 catcaccgga tttataagct atcagccgga ttttttagtt atacgtttag ttatacgggc
    94861 tgacgtataa ctaaagccgt ataactaaaa ccctgttttt tcgtttggaa tcataagcat
    94921 atggcgagaa aaacaccccc attaaccaca gtacagatca aagcagccag accagcagaa
    94981 aaggaataca ccctacagga cggcggaggg ctttttctcc tgatcaaacc gtccggatca
    95041 aaactctgga gattttccta ctaccgacca tcggacaaaa aaagaatatt gctgagtttt
    95101 ggatcgcttg atgatgtttc cctggctgat gccagaaaac gccgtagcga gtacagggcg
    95161 ttaattcgtg ccggaactga cccgcaggac cacgagaggc aaaaaagaga gacagaggcc
    95221 cgaagacaag ggaacacgtt cgaaaatgtg gcaacagcat ggtaccaggt gaaaatcagc
    95281 cagaatctgg cccccaatac gattaaagac atctggcgat cgctggaaaa atatgtattc
    95341 ccgttcatcg gcaacacgcc aatagacacc ctgaccgctc gcaggtttgt tgaggtgctt
    95401 acccccatca aggaacgtgg caacctggaa acgctcaagc gagttttaca gcgtgtaaat
    95461 gaggtaatgg attacgccgc caatagtggg atgattgatg ccaatccggc tatgaatgtg
    95521 cgtaaggcgt tcccctcccc tgtaaaaaaa catatgccaa caatccgccc cgaacagctg
    95581 ccggagctta tgcaggcttt atcagtatcg gcaacagaac ggcagaccag attactgatt
    95641 gaatggcagt tactgaccgt aacccgtccc gttgaagcat cgtcggcacg ctgggaagaa
    95701 atcgacatgg aggcgcaacg ctggaccata cccgccggac gcatgaagat gcgccgcgac
    95761 cacgttatcc cgctttgtga ccaggctatg gcagtgctgg aggctatgaa accaatcagc
    95821 caccaccgcg attatgtttt cccaagtctg aaagacccac ggcagccgat gaacagccag
    95881 acagcaaacg cagcattacg acgcatgggg ttcgctggcg tgctggtgtc tcatggatta
    95941 cgcgccatat tcagcacagc agcgaacgag gaaggattcg agccggacgt aatcgaggcg
    96001 gcacttgccc acgtcgacac caacgaagtc agacgggcat acaaccggag caactacata
    96061 gaaaaacgcg tggtgcttat gcgctggtgg ggcgaatttg tcgaggctgc ggcgacgggc
    96121 gtaaccctcg ccagtggtaa aaggggtatc cgagccgttt agctgtacag aaaaccagta
    96181 aaaactacga aaaccattta aaaccgtcgt ataattgcat cacatttaac gacgaggcct
    96241 tgaaaaatga aaccgttaag atgcaaacaa atttcagatg caattgcgac gggctgcaac
    96301 tggccctgat ggttcagcat gaattttggt caacctacga tccggaggac agaacgacgg
    96361 ccccatcaaa aaaacaggta gtagatttcc tggtatcccg tggagcttcc agaaatctgg
    96421 ctgtaagtat tgataaggtt gtacgtccgg catccatgaa gaccggaggc aggcccaaaa
    96481 aatggcggta acaatcctga aagcggcaga aatgccgctt tttttataaa tccctttcaa
    96541 atcatcaata taaaaaacgg tgtataccgt ttaaaaacgg tgagtactgt ttttccctgc
    96601 atccgatgat ttaccgtatt tctcaccgga atacaccgga ttaatgaggt aaatcacgat
    96661 ggaagcaatc aaaaaagtca tctttcgcca ggaagtaaaa aaacttatcc atattaaggc
    96721 agacagcacg ctgcaaagca tgatcaacgc cggagaattc ccgcagggtt ttcgtgtcgg
    96781 tttgcgccgt cgcggatggt atgaggaaga cgtaatcgca tggatgaaag aacgcgaaca
    96841 agaagcgcgc ggaacgaccg cttaacatat atagggcaaa tgatgaacat aaaaaaagcg
    96901 gccccgaaat ggagccacct ttctgaacaa ttaacccgct gcgccgtatg tgtcaacaat
    96961 cataagcctg ggcatgataa ccgcgatcag gctggtgggc aattatcttg cttgttgggg
    97021 ccgtattgtg ctgccatttt catccgttca gaacgtgaat atttttttag aaactcatca
    97081 aggacaaaag cgcatggtgc gaatctttct gattcatgct cgagcgccat attttcgtgc
    97141 tctctgttcg ttttttgcga tggtgtattg gttgattctt tattggtcat tgtgtgtacc
    97201 tgtaaagcaa tgcgccgtag tacctcacgc cacggcgctg atgtttttta tcctttgggt
    97261 tcaataccgc gttgctgtaa ttcttttcgg agtactcgct tgatccaggt ggctaaagaa
    97321 gtgtcaccgt ctgctttcgc tgcctcttca agttgtgctc tgaattcttc tggtagccgc
    97381 atttggtatg gaggcgatcg cttttccaat ggtgttgaca tggtaattac ccttaattac
    97441 tatagttaca tggtaattac cattgtactt accgttatac aaaaaacaac gccccgcagt
    97501 ggtggctcac atacggggcg tctaaccaaa ccattaattg aggtaacgat tatggctata
    97561 aaagagcata gcttactctt tatacacaca caaacgcccc ggaaacggaa taatccgttt
    97621 catagctccg ttttgtgtac tcatcctggc gggcgattct tacgggactt tagccgccgt
    97681 gggcagcttg ctaaaaatgc ctccaacgcg aatagtccag gtttcatttc tatcggtatt
    97741 agttggatat tcagaggatt agccagttcg gcttcaaggc gagcacaggt ttctaactgc
    97801 ctttgccgaa tgatttcatc ttcttgcgtc tgcgtgtctt gtattggtga tagtgtgttg
    97861 ttggtcatag tactgccctg taaagcaatg cgccgtagta cctcacacca cggcgctgat
    97921 agtgattatt ctgattcttt ggccttgcgg cgctggagtt cttcacgtgc gacagtgacg
    97981 agctgcccga tctcctcggc ggctttgatt ccgagttttt ccacctgcgc cagtgcattg
    98041 agcgaagaaa tcaggaggtt ttctccgctt ccttctgcct ggcggcgggc gatttcaccg
    98101 cgcatggcgg ttactatgaa tccggcgttg ctttcaccgt ccagttttac ggattccatg
    98161 ccttcaataa catcatgcgg gatccgagct gtcagtgatt gtgattttgc gttttttgaa
    98221 cctgtagcca tctgtaatcc tctcaatgaa agtgtaagac aatatacaca taaaaagtct
    98281 tacataaaag cattgacatg taagccacct ataaataaag ttacttacac cttgttaatg
    98341 caaggtgcag aaacgacgaa accccgcact gtaggagcag taacggggct tctaaccacc
    98401 aacgataacg agagtatcga ggtagctatg agaaatcata ccacacaccc gcaagggcgg
    98461 gactcgcaca acctgaataa atacatttgg cgttttatcg ccctgagcac
//
LOCUS       DAZGTR010000004        89461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGTR010000004 DAZGTR010000000
VERSION     DAZGTR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.004"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..389)
                     /locus_tag="ACHLCY_000287"
     CDS             complement(<1..389)
                     /locus_tag="ACHLCY_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0367614.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPG"
     gene            complement(549..761)
                     /gene="rpmE"
                     /locus_tag="ACHLCY_000288"
     CDS             complement(549..761)
                     /gene="rpmE"
                     /locus_tag="ACHLCY_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP0367615.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            964..3162
                     /gene="priA"
                     /locus_tag="ACHLCY_000289"
     CDS             964..3162
                     /gene="priA"
                     /locus_tag="ACHLCY_000289"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP0367616.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3318..4343
                     /gene="cytR"
                     /locus_tag="ACHLCY_000290"
     CDS             3318..4343
                     /gene="cytR"
                     /locus_tag="ACHLCY_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP0367617.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4435..5394
                     /gene="ftsN"
                     /locus_tag="ACHLCY_000291"
     CDS             4435..5394
                     /gene="ftsN"
                     /locus_tag="ACHLCY_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP0367618.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQPPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5487..6017
                     /gene="hslV"
                     /locus_tag="ACHLCY_000292"
     CDS             5487..6017
                     /gene="hslV"
                     /locus_tag="ACHLCY_000292"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP0367619.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6027..7358
                     /gene="hslU"
                     /locus_tag="ACHLCY_000293"
     CDS             6027..7358
                     /gene="hslU"
                     /locus_tag="ACHLCY_000293"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP0367620.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7425..8351
                     /gene="menA"
                     /locus_tag="ACHLCY_000294"
     CDS             7425..8351
                     /gene="menA"
                     /locus_tag="ACHLCY_000294"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP0367621.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8444..8929
                     /gene="rraA"
                     /locus_tag="ACHLCY_000295"
     CDS             8444..8929
                     /gene="rraA"
                     /locus_tag="ACHLCY_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP0367622.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9014..9259)
                     /gene="zapB"
                     /locus_tag="ACHLCY_000296"
     CDS             complement(9014..9259)
                     /gene="zapB"
                     /locus_tag="ACHLCY_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP0367623.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9684..10529
                     /gene="glpF"
                     /locus_tag="ACHLCY_000297"
     CDS             9684..10529
                     /gene="glpF"
                     /locus_tag="ACHLCY_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP0367624.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10552..12060
                     /gene="glpK"
                     /locus_tag="ACHLCY_000298"
     CDS             10552..12060
                     /gene="glpK"
                     /locus_tag="ACHLCY_000298"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP0367625.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12195..13205
                     /gene="glpX"
                     /locus_tag="ACHLCY_000299"
     CDS             12195..13205
                     /gene="glpX"
                     /locus_tag="ACHLCY_000299"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0367626.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13302..14048
                     /gene="fpr"
                     /locus_tag="ACHLCY_000300"
     CDS             13302..14048
                     /gene="fpr"
                     /locus_tag="ACHLCY_000300"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP0367627.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14053..14481)
                     /gene="uspD"
                     /locus_tag="ACHLCY_000301"
     CDS             complement(14053..14481)
                     /gene="uspD"
                     /locus_tag="ACHLCY_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP0367628.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14508..14807)
                     /gene="yiiS"
                     /locus_tag="ACHLCY_000302"
     CDS             complement(14508..14807)
                     /gene="yiiS"
                     /locus_tag="ACHLCY_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP0367629.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15019..15459)
                     /gene="yiiR"
                     /locus_tag="ACHLCY_000303"
     CDS             complement(15019..15459)
                     /gene="yiiR"
                     /locus_tag="ACHLCY_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0367630.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15560..16159
                     /gene="yiiQ"
                     /locus_tag="ACHLCY_000304"
     CDS             15560..16159
                     /gene="yiiQ"
                     /locus_tag="ACHLCY_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP0367631.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16267..17034
                     /gene="tpiA"
                     /locus_tag="ACHLCY_000305"
     CDS             16267..17034
                     /gene="tpiA"
                     /locus_tag="ACHLCY_000305"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP0367632.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17089..17844)
                     /gene="cdh"
                     /locus_tag="ACHLCY_000306"
     CDS             complement(17089..17844)
                     /gene="cdh"
                     /locus_tag="ACHLCY_000306"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP0367633.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAK
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(17951..18940)
                     /gene="sbp"
                     /locus_tag="ACHLCY_000307"
     CDS             complement(17951..18940)
                     /gene="sbp"
                     /locus_tag="ACHLCY_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP0367634.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19259..20221)
                     /gene="pfkA"
                     /locus_tag="ACHLCY_000308"
     CDS             complement(19259..20221)
                     /gene="pfkA"
                     /locus_tag="ACHLCY_000308"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP0367635.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20402..21304)
                     /gene="fieF"
                     /locus_tag="ACHLCY_000309"
     CDS             complement(20402..21304)
                     /gene="fieF"
                     /locus_tag="ACHLCY_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP0367636.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21453..21953)
                     /gene="cpxP"
                     /locus_tag="ACHLCY_000310"
     CDS             complement(21453..21953)
                     /gene="cpxP"
                     /locus_tag="ACHLCY_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP0367637.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22103..22801
                     /gene="cpxR"
                     /locus_tag="ACHLCY_000311"
     CDS             22103..22801
                     /gene="cpxR"
                     /locus_tag="ACHLCY_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP0367638.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22798..24171
                     /gene="cpxA"
                     /locus_tag="ACHLCY_000312"
     CDS             22798..24171
                     /gene="cpxA"
                     /locus_tag="ACHLCY_000312"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP0367639.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24277..24951)
                     /gene="yiiM"
                     /locus_tag="ACHLCY_000313"
     CDS             complement(24277..24951)
                     /gene="yiiM"
                     /locus_tag="ACHLCY_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP0367640.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25100..26083)
                     /gene="kdgT"
                     /locus_tag="ACHLCY_000314"
     CDS             complement(25100..26083)
                     /gene="kdgT"
                     /locus_tag="ACHLCY_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP0367641.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNRDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26343..26963)
                     /gene="sodA"
                     /locus_tag="ACHLCY_000315"
     CDS             complement(26343..26963)
                     /gene="sodA"
                     /locus_tag="ACHLCY_000315"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP0367642.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27248..28282
                     /gene="rhaT"
                     /locus_tag="ACHLCY_000316"
     CDS             27248..28282
                     /gene="rhaT"
                     /locus_tag="ACHLCY_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP0367643.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWSNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28279..29127)
                     /gene="rhaR"
                     /locus_tag="ACHLCY_000317"
     CDS             complement(28279..29127)
                     /gene="rhaR"
                     /locus_tag="ACHLCY_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP0367644.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQHVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQLAMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(29201..30037)
                     /gene="rhaS"
                     /locus_tag="ACHLCY_000318"
     CDS             complement(29201..30037)
                     /gene="rhaS"
                     /locus_tag="ACHLCY_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP0367645.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYHCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30325..31794
                     /gene="rhaB"
                     /locus_tag="ACHLCY_000319"
     CDS             30325..31794
                     /gene="rhaB"
                     /locus_tag="ACHLCY_000319"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP0367646.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDNF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31791..33050
                     /gene="rhaA"
                     /locus_tag="ACHLCY_000320"
     CDS             31791..33050
                     /gene="rhaA"
                     /locus_tag="ACHLCY_000320"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP0367647.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            33133..33957
                     /gene="rhaD"
                     /locus_tag="ACHLCY_000321"
     CDS             33133..33957
                     /gene="rhaD"
                     /locus_tag="ACHLCY_000321"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP0367648.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAEVLVKIYSMGGMKQTITREELIALGERFGVTPLASALAL"
     gene            34029..35177
                     /gene="fucO"
                     /locus_tag="ACHLCY_000322"
     CDS             34029..35177
                     /gene="fucO"
                     /locus_tag="ACHLCY_000322"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP0367649.1"
                     /translation="MSFMLALPKISLHGAGAIGDMVNLVAHKQWGKALIVTDGQLVKL
                     GLLDSLFIALDQYEMSYHLFDEVFPNPTEELVQKGYAAFQDAACDYIIAFGGDSPIDT
                     AKAVKILTANPAPSTAYSGVGKVTNPGVPLVAINTTAGTAAEMTSNAVIIDTARQVKE
                     VIIDPNIIPDIAVDDASVMLEIPASVTAATGMDALTHAVEAYVSVGAHPLTDANALEA
                     IRLITLWLPKAVDDGHNLEAREQMAFAQYLAGMAFNSAGLGLVHALAHQPGATHNLPH
                     GVCNAILLPVIENFNRPNAIARFARIAQAMGVDTRDMSEEAASTEAINAIRALSHRVG
                     IPAGFSQLGITKEDIEGWLDKALADPCVPCNPRTASRDEVRELYLEAL"
     gene            35174..35488
                     /gene="rhaM"
                     /locus_tag="ACHLCY_000323"
     CDS             35174..35488
                     /gene="rhaM"
                     /locus_tag="ACHLCY_000323"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP0367650.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            35801..36247
                     /gene="frvA"
                     /locus_tag="ACHLCY_000324"
     CDS             35801..36247
                     /gene="frvA"
                     /locus_tag="ACHLCY_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP0367651.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFINQLRQGDADQVLALLNQTLSS"
     gene            36258..37709
                     /gene="frvB"
                     /locus_tag="ACHLCY_000325"
     CDS             36258..37709
                     /gene="frvB"
                     /locus_tag="ACHLCY_000325"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP0367652.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIIGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            37699..38769
                     /gene="frvX"
                     /locus_tag="ACHLCY_000326"
     CDS             37699..38769
                     /gene="frvX"
                     /locus_tag="ACHLCY_000326"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0367653.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPQSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGNGPGLMLFDKRYFPNQKLVAALKSCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVSAFSQFRQVD"
     gene            38769..40517
                     /gene="frvR"
                     /locus_tag="ACHLCY_000327"
     CDS             38769..40517
                     /gene="frvR"
                     /locus_tag="ACHLCY_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP0367654.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(40567..41607)
                     /gene="yiiG"
                     /locus_tag="ACHLCY_000328"
     CDS             complement(40567..41607)
                     /gene="yiiG"
                     /locus_tag="ACHLCY_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP0367655.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDSVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
     gene            complement(41760..42593)
                     /gene="fdhD"
                     /locus_tag="ACHLCY_000329"
     CDS             complement(41760..42593)
                     /gene="fdhD"
                     /locus_tag="ACHLCY_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP0367656.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            42787..45837
                     /gene="fdnG"
                     /locus_tag="ACHLCY_000330"
     CDS             42787..45837
                     /gene="fdnG"
                     /locus_tag="ACHLCY_000330"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:43372..43374,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0367657.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            45850..46752
                     /gene="fdoH"
                     /locus_tag="ACHLCY_000331"
     CDS             45850..46752
                     /gene="fdoH"
                     /locus_tag="ACHLCY_000331"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP0367658.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPEARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            46749..47384
                     /gene="fdoI"
                     /locus_tag="ACHLCY_000332"
     CDS             46749..47384
                     /gene="fdoI"
                     /locus_tag="ACHLCY_000332"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0367659.1"
                     /translation="MKRRDTILRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            47381..48310
                     /gene="fdhE"
                     /locus_tag="ACHLCY_000333"
     CDS             47381..48310
                     /gene="fdhE"
                     /locus_tag="ACHLCY_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP0367660.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(48641..48883)
                     /gene="yiiF"
                     /locus_tag="ACHLCY_000334"
     CDS             complement(48641..48883)
                     /gene="yiiF"
                     /locus_tag="ACHLCY_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFP0367661.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(49102..49320)
                     /gene="yiiE"
                     /locus_tag="ACHLCY_000335"
     CDS             complement(49102..49320)
                     /gene="yiiE"
                     /locus_tag="ACHLCY_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0367662.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(49925..50215)
                     /gene="nadS"
                     /locus_tag="ACHLCY_000336"
     CDS             complement(49925..50215)
                     /gene="nadS"
                     /locus_tag="ACHLCY_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0367663.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(50216..50527)
                     /locus_tag="ACHLCY_000337"
     CDS             complement(50216..50527)
                     /locus_tag="ACHLCY_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0367664.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            50756..51664
                     /locus_tag="ACHLCY_000338"
     CDS             50756..51664
                     /locus_tag="ACHLCY_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0367665.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(51728..52669)
                     /gene="fabY"
                     /locus_tag="ACHLCY_000339"
                     /gene_synonym="yiiD"
     CDS             complement(51728..52669)
                     /gene="fabY"
                     /locus_tag="ACHLCY_000339"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP0367666.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(52714..53151)
                     /gene="dtd"
                     /locus_tag="ACHLCY_000340"
     CDS             complement(52714..53151)
                     /gene="dtd"
                     /locus_tag="ACHLCY_000340"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP0367667.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(53148..54020)
                     /gene="yihY"
                     /locus_tag="ACHLCY_000341"
     CDS             complement(53148..54020)
                     /gene="yihY"
                     /locus_tag="ACHLCY_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP0367668.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(54014..54613)
                     /gene="yihX"
                     /locus_tag="ACHLCY_000342"
     CDS             complement(54014..54613)
                     /gene="yihX"
                     /locus_tag="ACHLCY_000342"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP0367669.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(54712..55497)
                     /gene="csqR"
                     /locus_tag="ACHLCY_000343"
     CDS             complement(54712..55497)
                     /gene="csqR"
                     /locus_tag="ACHLCY_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0367670.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(55531..56427)
                     /gene="yihV"
                     /locus_tag="ACHLCY_000344"
     CDS             complement(55531..56427)
                     /gene="yihV"
                     /locus_tag="ACHLCY_000344"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFP0367671.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELMALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            56595..57491
                     /gene="yihU"
                     /locus_tag="ACHLCY_000345"
     CDS             56595..57491
                     /gene="yihU"
                     /locus_tag="ACHLCY_000345"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP0367672.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            57515..58393
                     /gene="yihT"
                     /locus_tag="ACHLCY_000346"
     CDS             57515..58393
                     /gene="yihT"
                     /locus_tag="ACHLCY_000346"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFP0367673.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            58410..59651
                     /gene="yihS"
                     /locus_tag="ACHLCY_000347"
     CDS             58410..59651
                     /gene="yihS"
                     /locus_tag="ACHLCY_000347"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFP0367674.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            59789..60691
                     /gene="yihR"
                     /locus_tag="ACHLCY_000348"
     CDS             59789..60691
                     /gene="yihR"
                     /locus_tag="ACHLCY_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFP0367675.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANLVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            60890..62926
                     /gene="yihQ"
                     /locus_tag="ACHLCY_000349"
     CDS             60890..62926
                     /gene="yihQ"
                     /locus_tag="ACHLCY_000349"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFP0367676.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENADGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDNDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            62972..64357
                     /gene="yihP"
                     /locus_tag="ACHLCY_000350"
     CDS             62972..64357
                     /gene="yihP"
                     /locus_tag="ACHLCY_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0367677.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            64400..65803
                     /gene="yihO"
                     /locus_tag="ACHLCY_000351"
     CDS             64400..65803
                     /gene="yihO"
                     /locus_tag="ACHLCY_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0367678.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            65871..66563
                     /gene="ompL"
                     /locus_tag="ACHLCY_000352"
     CDS             65871..66563
                     /gene="ompL"
                     /locus_tag="ACHLCY_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFP0367679.1"
                     /translation="MKKINAIILLSSLTSASVFAGTYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(66654..67919)
                     /gene="yihN"
                     /locus_tag="ACHLCY_000353"
     CDS             complement(66654..67919)
                     /gene="yihN"
                     /locus_tag="ACHLCY_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0367680.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(68021..69001)
                     /gene="yihM"
                     /locus_tag="ACHLCY_000354"
     CDS             complement(68021..69001)
                     /gene="yihM"
                     /locus_tag="ACHLCY_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP0367681.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDVRLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(69009..69719)
                     /gene="yihL"
                     /locus_tag="ACHLCY_000355"
     CDS             complement(69009..69719)
                     /gene="yihL"
                     /locus_tag="ACHLCY_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0367682.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(69935..71758)
                     /gene="typA"
                     /locus_tag="ACHLCY_000356"
     CDS             complement(69935..71758)
                     /gene="typA"
                     /locus_tag="ACHLCY_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP0367683.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            72131..73540
                     /gene="glnA"
                     /locus_tag="ACHLCY_000357"
     CDS             72131..73540
                     /gene="glnA"
                     /locus_tag="ACHLCY_000357"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP0367684.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            73859..74908
                     /gene="glnL"
                     /locus_tag="ACHLCY_000358"
     CDS             73859..74908
                     /gene="glnL"
                     /locus_tag="ACHLCY_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP0367685.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            74920..76329
                     /gene="glnG"
                     /locus_tag="ACHLCY_000359"
     CDS             74920..76329
                     /gene="glnG"
                     /locus_tag="ACHLCY_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP0367686.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDNWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            76441..76551
                     /gene="yshB"
                     /locus_tag="ACHLCY_000360"
     CDS             76441..76551
                     /gene="yshB"
                     /locus_tag="ACHLCY_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP0367687.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(76741..78114)
                     /gene="hemN"
                     /locus_tag="ACHLCY_000361"
     CDS             complement(76741..78114)
                     /gene="hemN"
                     /locus_tag="ACHLCY_000361"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP0367688.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(78303..78812)
                     /gene="yihI"
                     /locus_tag="ACHLCY_000362"
     CDS             complement(78303..78812)
                     /gene="yihI"
                     /locus_tag="ACHLCY_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP0367689.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(78931..79097)
                     /locus_tag="ACHLCY_000363"
                     /pseudo
     CDS             complement(78931..79097)
                     /locus_tag="ACHLCY_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            79394..80026
                     /gene="yihA"
                     /locus_tag="ACHLCY_000364"
     CDS             79394..80026
                     /gene="yihA"
                     /locus_tag="ACHLCY_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP0367690.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(80408..83194)
                     /gene="polA"
                     /locus_tag="ACHLCY_000365"
     CDS             complement(80408..83194)
                     /gene="polA"
                     /locus_tag="ACHLCY_000365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP0367691.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            83558..84490
                     /gene="yihG"
                     /locus_tag="ACHLCY_000366"
     CDS             83558..84490
                     /gene="yihG"
                     /locus_tag="ACHLCY_000366"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0367692.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDQLLTSLMSSQRQDK"
     gene            complement(84531..85960)
                     /gene="yihF"
                     /locus_tag="ACHLCY_000367"
                     /pseudo
     CDS             complement(84531..85960)
                     /gene="yihF"
                     /locus_tag="ACHLCY_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(86115..86741)
                     /gene="dsbA"
                     /locus_tag="ACHLCY_000368"
     CDS             complement(86115..86741)
                     /gene="dsbA"
                     /locus_tag="ACHLCY_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP0367693.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(86758..87744)
                     /gene="srkA"
                     /locus_tag="ACHLCY_000369"
     CDS             complement(86758..87744)
                     /gene="srkA"
                     /locus_tag="ACHLCY_000369"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP0367694.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(87821..88090)
                     /gene="yihD"
                     /locus_tag="ACHLCY_000370"
     CDS             complement(87821..88090)
                     /gene="yihD"
                     /locus_tag="ACHLCY_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP0367695.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            88160..88744
                     /gene="mobA"
                     /locus_tag="ACHLCY_000371"
     CDS             88160..88744
                     /gene="mobA"
                     /locus_tag="ACHLCY_000371"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP0367696.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            88726..89253
                     /gene="mobB"
                     /locus_tag="ACHLCY_000372"
     CDS             88726..89253
                     /gene="mobB"
                     /locus_tag="ACHLCY_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP0367697.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(89357..>89461)
                     /gene="rrf"
                     /locus_tag="ACHLCY_000373"
     rRNA            complement(89357..>89461)
                     /gene="rrf"
                     /locus_tag="ACHLCY_000373"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        21888 a        23009 c        22514 g        22050 t
ORIGIN      
        1 tcaccgggaa acaggtgctc aaaatacagg cttctgccgc cgttatcact gagtatcagt
       61 gtgttatcgc gcagctgtaa gcggatatcc gcaggcattt tccagccggg gccgaggctc
      121 cccaccggcg cgggcgtttt tgtccggtaa ctgctgtagg tgcgggagag gatgaacggc
      181 agcgggccgg gcagggcgat gtcggtttca ccgggaagga cctttgcacc gagcagggga
      241 ttgaccggat ggccggacgt cactccgccg gggcacaccg aacaggccac gccggtgggg
      301 gcaccaatgc gcaccccggc tgaaccctga acaatgctac cgccatactg cgtcatgtcg
      361 ccctgacgcg ccgccggttt tccgctcatc gtaaaatcct tttgaaataa ataagaacca
      421 gaagcctagc gccagtcaac aacaggaaat gataatgagt taacaccccg gaatttttca
      481 gaatttataa aaggcaaatt acaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
      541 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
      601 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
      661 aggttcaggt catgaccaac ggtggagcgg attttcatta cgttaccgca agagcagcta
      721 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
      781 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
      841 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
      901 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
      961 gctatgcccg tagcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
     1021 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
     1081 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcaatgag
     1141 ctaaaagcgg tagtcgaagt gctggacgtt gagccggtat ttactcactc cgtctggcgg
     1201 ttactgctct gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
     1261 ttgccgattt tactgcgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
     1321 gccactgaac aaggtcaggc ggtggatctt aacagcctga aacgttcgcc aaagcaacaa
     1381 caggcgctgg cggcgttacg gcaaggcaaa atctggcgcg accaggtcgc ggaacttgaa
     1441 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
     1501 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
     1561 aataccgaac aagccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
     1621 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
     1681 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
     1741 caaactatcg cccgttttcg tgaacgtttt aatgcccccg tggaagttct gcattccggc
     1801 ctgaacgaca gcgagcggct ttcggcgtgg ttgaaagcga aaaatggcga ggcggcgatt
     1861 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
     1921 gatgaagagc acgacagctc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
     1981 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
     2041 gcgctggaaa cgttatgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacccgt
     2101 cgggcaggga atgcgcgtcc ggcaattcaa catgtgctgg atttaaaagg tcagaaggtg
     2161 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
     2221 caggttatcc tctttcttaa tcgtcggggt tttgcgcctg cgctgctgtg ccacgactgc
     2281 ggctggatag ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
     2341 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
     2401 ggttccacgc acctggtccc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
     2461 ccgatgttcc ccggcgtgcc catttcacgt atcgaccgcg ataccaccag ccgcaaaggg
     2521 gcgctggaac agcaactggc ggaagtgcat cgcggcggcg cgaggatctt gattggcacg
     2581 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
     2641 gacggcgcgc tgttttctgc cgattttcgc tcggcagaac gtttcgccca gctttacacc
     2701 caggtcgccg gtcgtgccgg gcgtgcgggt aaacagggcg aagtggtgct gcaaacgcac
     2761 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgacgc ctttgccgaa
     2821 caggcgctgg ctgagcgacg catgatgcag ctgccgccgt ggaccagcca tgtgattgtg
     2881 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
     2941 atcctttcca gcccgctggc agacgacaaa ctgtgggttc tcggcccggt tccggctctg
     3001 gcacctaagc gtggtggccg ctggcgctgg cagatcttgt tgcagcaccc ttcccgcgtg
     3061 cgcttgcaac acatcattag tggtacgctg gcgcttatta atacaatacc ggattcccgc
     3121 aaggtgaaat gggtgttgga tgtcgatccc atcgaaggct aatgcgcaaa cgttgcgagg
     3181 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
     3241 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
     3301 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
     3361 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
     3421 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
     3481 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
     3541 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
     3601 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
     3661 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
     3721 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
     3781 tcgcaccgga actggagcta cctacagttc atatcgacaa tctgaccgcc gcatttgatg
     3841 cagtaaatta tctatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
     3901 agatgccgct gtgtcactac cgcctgcaag gttacgttca ggcgctgcgt cgctgcggca
     3961 ttatggttga tccgcaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
     4021 cgatgcagca gctgcttgat cttccacaac cgcctactgc tgtcttctgc catagcgatg
     4081 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
     4141 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
     4201 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
     4261 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
     4321 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
     4381 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
     4441 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
     4501 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
     4561 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
     4621 gagacgctgc aaagccagaa agtgaccggt aacggactac caccaaaacc agaagaacgc
     4681 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
     4741 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
     4801 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaagt tccgtggaat
     4861 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
     4921 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
     4981 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accggcttct
     5041 actcagcagc cgtaccagga tctgctgcaa ccccctgcgc acacgaccgc gcagtcgaag
     5101 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
     5161 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
     5221 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
     5281 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
     5341 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
     5401 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
     5461 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
     5521 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
     5581 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
     5641 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
     5701 tggtcaaagc cgccgttgag ctggcgaaag actggcgtac cgatcgcatg ctgcgcaaac
     5761 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
     5821 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
     5881 ctgcggcgcg tgcactgtta gaaaatactg aactgagtgc tcgtgaaatt gctgaaaagg
     5941 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
     6001 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
     6061 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
     6121 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
     6181 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
     6241 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa attcaccgaa gtaggctacg
     6301 tcggtaagga agtggattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
     6361 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
     6421 tcgacgtgct gatcccacct gctaaaaaca actggggaca gaccgaacag cagcaggaac
     6481 cgtccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
     6541 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccaggca
     6601 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
     6661 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
     6721 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
     6781 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
     6841 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
     6901 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
     6961 ttgcgaaacc gtctgaccta atcccggaac tgcaaggtcg tctgccaatc cgcgttgaac
     7021 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
     7081 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
     7141 gtattaagcg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
     7201 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
     7261 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
     7321 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
     7381 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
     7441 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
     7501 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
     7561 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
     7621 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
     7681 aaaaaggggt cattacccag caagagatga aacgggcgct cattattacc gtcgtgctca
     7741 tctgtctctc cgggttggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
     7801 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
     7861 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
     7921 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
     7981 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
     8041 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
     8101 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
     8161 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
     8221 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
     8281 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
     8341 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
     8401 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
     8461 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
     8521 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
     8581 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
     8641 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
     8701 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
     8761 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
     8821 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
     8881 cgacaatacc gggattattc tttctgaaga tccgctggat attgaatgat agaaaaggca
     8941 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgga
     9001 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
     9061 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
     9121 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
     9181 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
     9241 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaatcctc
     9301 gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta
     9361 tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt
     9421 catccttctc ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg
     9481 aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaatggcgc gataacgctc
     9541 attttatgac aaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga
     9601 taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa
     9661 cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg
     9721 ctgaattcct cggtaccggg ttgttgattt tcttcggtgt gggttgcgtt gcagcactaa
     9781 aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg
     9841 caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca
     9901 ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac
     9961 aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt
    10021 tcgacttcga gcagactcat cacattgttc gcggcagcgt tgaaagtgtt gatctggctg
    10081 gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga
    10141 tggtgattac cgctattctg atggggctga tcctggcgtt aacggatgat ggcaacggtg
    10201 taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat
    10261 ctatgggccc attgacaggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct
    10321 ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact
    10381 tcctggtgcc gcttttcggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca
    10441 aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gaaaccacaa
    10501 ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa
    10561 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    10621 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    10681 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    10741 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    10801 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    10861 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    10921 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    10981 tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga attgctgttt
    11041 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    11101 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    11161 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    11221 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    11281 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    11341 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    11401 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    11461 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    11521 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    11581 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    11641 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    11701 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    11761 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    11821 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    11881 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    11941 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    12001 tacgcaggct ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgaataa
    12061 tgtaaatgcc gaatgaagcg tttatgccgc atccggtagt cccgaaacgt gcgggggcaa
    12121 ccccgcacac atcaataatc cctcccttcc cctgtgctac acttcgcgcc attccttact
    12181 gcttagagtt tgctatgaga cgagaacttg ccatcgaatt ttcccgcgtc accgaatcag
    12241 cggcgctggc tggctacaaa tggttaggac gcggcgataa aaacaccgcg gacggcgcgg
    12301 cggtaaacgc catgcgtatt atgctcaacc aggtcaacat tgacggcacc atcgtcattg
    12361 gtgaaggtga aatcgacgaa gcaccgatgc tctacattgg tgaaaaagtc ggtactggtc
    12421 gcggcgacgc ggtagatatt gctgttgatc cgattgaagg cacgcgcatg acggcgatgg
    12481 gccaggctaa cgcgctggcg gtgctggcag taggcgataa aggctgcttc ctcaatgcgc
    12541 cggatatgta tatggagaag ctgattgtcg ggccgggagc caaaggcacc attgatctga
    12601 acctgccgct ggcggataac ctgcgcaatg tagcggcggc gctcggcaaa ccgttgagcg
    12661 aactgacggt aacgattctg gctaaaccac gccacgatgc cgttatcgct gaaatgcagc
    12721 aactcggcgt acgcgtattt gctattccgg atggcgacgt tgcggcctca attctcacct
    12781 gtatgccaga cagcgaagtt gacgtgctgt acggtattgg tggcgcgccg gaaggcgtag
    12841 tttctgcggc ggtgatccgc gcattagatg gcgacatgaa cggtcgtctg ctggcgcgtc
    12901 atgacgtcaa aggcgacaac gaagagaatc gtcgcattgg cgagcaggag ctggcacgct
    12961 gcaaagcgat gggcatcgaa gccggtaaag tattgcgcct gggcgatatg gcgcgcagcg
    13021 ataacgtcat cttctctgcc accggtatta ccaaaggcga tctgctggaa ggcattagcc
    13081 gcaaaggcaa tatcgcgact accgaaacgc tgctgatccg cggcaagtca cgcaccattc
    13141 gccgcattca gtccatccac tatctggatc gcaaagaccc ggaaatgcag gtgcacatcc
    13201 tctgattgat ttgatcgatt gagccttcca gtccttcggg actggaattt ttttgttcgg
    13261 agaacgaaga taaggcaagt caatcaaaac aggagaaaaa catggctgat tgggtaacag
    13321 gcaaagtcac taaagtgcag aactggaccg acgccctgtt tagtctcacc gttcacgccc
    13381 ccgtgcttcc gtttaccgcc gggcaattca ccaagcttgg ccttgaaatc gacggcgaac
    13441 gcgtccagcg cgcctactcc tatgttaact cgcccgataa tcccgatctg gagttttacc
    13501 tggtcaccgt ccccgatggc aaattaagcc cacgattggc ggcactgaaa ccaggcgatg
    13561 aagtgcaggt ggttagcgaa gcagctggct tctttgttct ggatgaagta ccggactgcg
    13621 aaacgctatg gatgctggca accggtacag cgattggccc ttatttatcg attctgcaac
    13681 taggcaaaga tttagatcgc ttcaaaaatc tggtcctggt tcacgccgca cgttatgccg
    13741 ccgacttaag ctatttgcca ctgatgcaag aactggaaaa acgctacgaa gggaaactgc
    13801 gcattcagac ggtggtcagt cgggaaacgg cagcggggtc gctcaccggg cggataccgg
    13861 cgttaattga aagtggggaa ctggaaagcg cgattggcct gccgatgaat aaagaaacca
    13921 gccatgtgat gctgtgcggc aatccacaga tggtgcgcga tacacaacag ttgctgaaag
    13981 agacccggca gatgacgaaa catttacgtc gccgaccggg ccatatgaca gcggagcatt
    14041 actggtaagc ggttacttat cgataaacgg cacgatgagc aaatccgcac tcatcttatt
    14101 gatcatcccg cgatatgccg gcatcaaacg gttgataaat gagtgatgat gaccacagac
    14161 aaggaggtcg cactgctctt tttgcataat ttccagcagt gtttccggca tttctccgcg
    14221 ttcaatacgc agttttgtct tcggccattg aatatttttc gtcagtttat acagcttgtt
    14281 atccgactta ttcttcaaca attgaagaat atcttctgtt gcagggaagt agatacccgg
    14341 atacaactcg cttaagccat catcaatatg aattaacgtc aggtgagcgt cattatgtct
    14401 ggcgagctcc agagctttat tcaccagtaa ggcatcttct tcattcccgg aaattgccac
    14461 gccaatgtgt ttataagcca tgtttaactc cttctaaagc aactccatca agctaacgaa
    14521 cgcagtgata actcaaaaat aatcatctcg gcctggcatg agaaagtgaa ggccgcatca
    14581 agctcaactt gcccctccag atccttaatc ttgtaggcca cgctggcccc ttcatctgca
    14641 gaggatgcag cggcggcaag ctctttcagt ttggtcataa aagactctgc ttcttcgcgg
    14701 gtggcaaaaa agcgcgaaaa cttactggta cagttgtcgt tatcaatcac cgtaccgata
    14761 tcaatcgcac atcctttagt actgcattta tctacgacat ctttcatagg gcacctctgt
    14821 atattcgcct ctctgtcgat tacgacttaa gaataatgcg ccgttaaggg aagaacaaac
    14881 aagtaatgat cttatggatt accatttttt ttgatattgc ttaaggtgaa gagttcagaa
    14941 ggattttccg ggtattgtca tttgcatcgt ggaaataaca atcccgaaaa aaaatagcag
    15001 taagagatat attaaaggtt aattacttga tttattgtcg gctttatact tcacatcctg
    15061 agtatcttta ccatatttat tttcgccttg tgtgccaaca aacgcaccaa gatcgataag
    15121 catcatcacc agaatcaacg tcgggacaaa acgccccacc gcccattgcc agacacccgg
    15181 taaaatcgcc cagttacccg ccagcagcat ccacgccaca atcatcagaa atgcccatgc
    15241 gccggaacgc ccgcgatcat gcaagcgctt aacagttact gccgctgttg gccaaagcaa
    15301 gcacacaagg caaaacgccg cggtctgaat atcgagtaaa tttttacccg ccagtgagaa
    15361 aagcaccagc atgcctgcga accacaggcc tatccaaatc cagaaatcac ggcgtccaat
    15421 acgcccttta aatgagaata accattgctg tatggtcatg taagttcctt gatggttgtc
    15481 ttttccagga ttctaccctt ttgacaaggg cgacagatat cgttttaatc ggagccagtc
    15541 ataacaaaag gtactgtcaa tgaagccagg gtgtacgctg ttttttctct tatgttctgc
    15601 attaaccgtt acaacaacgg cgcatgcaca aacaccagat acggcaacga ccgcgcctta
    15661 tctgctggct ggagccccta ctttcgatct ctccatcagc cagtttcgag aagactttaa
    15721 cagccagaat cctagcctgc cactgaacga atttcgtgcc atcgacagca gtcccgacaa
    15781 agccaatctc actcgtgctg caagtaaaat taacgagaac ttgtatgctt ctacagcgct
    15841 ggagcgcggt accttaaaaa tcaaaagcat tcaaatgacc tggctaccca tccaggggcc
    15901 agagcaaaaa gcagcgaaag cgaaagctca ggaatacatg gcagcagtga tccgcacact
    15961 caccccatta atgaccaaaa cacaaagcca gaaaaaactg cagtcgctac taacggcggg
    16021 gaaaaacaaa cgttattaca ccgagacaga aggtgcactg cgttatgttg tcgcggacaa
    16081 cggcgaaaag gggctgacct tcgctgttga accgattaag ctggcgctat ctgaatcgct
    16141 tgaaggtttg aataaatgac aaaaagcaaa gcctttgtgc cgatgaatct ctatactgtt
    16201 tcacagacct gctgccctgc ggggcggcca tcttccttta ttcgcttata agcgtggaga
    16261 attaaaatgc gacatccttt agtgatgggt aactggaaac tgaacggcag ccgccacatg
    16321 gttcacgagc tggtttctaa cctgcgtaaa gagctggcag gtgttgctgg ctgtgcggtt
    16381 gcaatcgcac caccggaaat gtacatcgat atggcgaagc gcgaagctga aggcagccac
    16441 atcatgctgg gtgcgcaaaa cgtggacctg aacctgtccg gcgcattcac cggtgaaact
    16501 tctgccgcta tgctgaaaga catcggcgct cagtacatca tcatcggcca ctctgaacgt
    16561 cgtacttacc acaaagagtc tgacgaactg attgcgaaaa aattcgcggt gctgaaagag
    16621 cagggcctga ctccggttct gtgcatcggt gaaaccgaag ctgaaaacga agcgggcaaa
    16681 actgaagaag tttgcgcacg tcagatcgac gcggtactga aaactcaggg tgctgcggca
    16741 ttcgaaggtg cggttatcgc ttacgaacct gtatgggcaa tcggtactgg caaatctgca
    16801 actccggctc aggcacaggc tgttcacaaa ttcatccgtg accacatcgc taaagttgac
    16861 gctaacatcg ctgaacaagt gatcattcag tacggcggct ctgtaaacgc gtctaacgct
    16921 gcagaactgt ttgctcagcc agatatcgac ggcgcgctgg ttggcggtgc ttctctgaaa
    16981 gctgacgctt tcgcagtgat cgttaaagct gcagaagcgg ctaaacaggc ttaagtctga
    17041 caggtgccgg atttcatatc cggcacttac tttccttaac tcctcgcctt aacgcaaaat
    17101 ctcacactca tgatcctgga tttcctcggc tgaagcacgg ttcagcgtca gtagatttcg
    17161 ttgtgtcgcc agcaacacaa acgaattatc actctgccgt accatcgcca gcccgtagct
    17221 tcccatatgt tcccgcgcct caggcacttc ttctgcaagc atcataaatg ggctgcgttg
    17281 taccagttcg ctttccgtta cccgacgcgc caggtattca tgcccgcgca aaccacctgg
    17341 cagtggcaac cagcggctgc tgattttcgc cagattttta tccagttgtt cgcgcacatc
    17401 aggacgaata caagagatat gaatatgaaa atggttttgc gtacgcccgg tgcgggagtt
    17461 gatcgccaaa gaaaccgcgc gatcgggaac cggctggccg tattttttgc tcataaaatc
    17521 acgcgcctgc caggccaacc aaaagaagtt cggcgttgaa ggatcggtca acaaaggact
    17581 ttcagtaccg ttaatacgat acgttggcat caacagatat tgcagtggac cattaagatc
    17641 ttttaaaacc acgtatccgg cattgggttt gacttccgca catggcgaag gattttgatt
    17701 ttgctgctga ttgggcaaac attcctcaag gacaatctta cgtaatgtat ccgactcttc
    17761 accggttaat ttccagtaac caataccggc agccacaacg gcgataacta tcatcaccaa
    17821 aaaaagaaga cccgcttttt tcatcttttt ttccctgtac ctcaaagaga tgcaagggta
    17881 acgcaaaatc gtgacaaata aaaaacccgg tcgcgagaaa ccaccgggtt tgataattat
    17941 cctggggaaa tcagcgtttg ctgatctgat cgaacgtacc gccgttagca aaatgctctt
    18001 tttgcgcttt cgtccagccg ccgaactctt catcaatggt gaataacttc agctttggaa
    18061 acgcattttc gtactttttc gctacttcag cgtcgcgcgg acggtagtag tttttcgcgg
    18121 caatttcctg accttctggc gagtagagat atttcaggta ggcttccgcc acctctttgg
    18181 tgcctttttt ctcgaccact ttatcgacca ccgacacggt tggctctgcg aggatagact
    18241 cactcggcgt gacgatttcg aatttatctt tccccagttc attcgctgcc agcagagctt
    18301 cgttttccca ggcgatcagt acatcgccaa tcccgcgctc gacaaaggtg ttagtggagc
    18361 cgcgcgcgcc agaatccaga acttcgacgt ttttatacag tgcccgaaca aaatcctgtg
    18421 cttttgcctg atcgttgttg ttgtgatgca gcgcgtagcc ccaggcagcc aggtagttcc
    18481 agcgtgcgcc accagagctt ttcggattag gcgtgatcac cgaaacaccc ggtttaatca
    18541 gatcgttcca gtcatggatc tgcttcggat tgcccttacg caccaggaaa acaatggtgg
    18601 aagtgtacgg tgcggagtta tccggcagac gtttgatcca ctctttatca atccgcccgc
    18661 gttccgcaat cgcgtccacg tcataggcca gagccagcgt gacaacatca gcttcaatac
    18721 cgttgattac cgacgtcgct tgtttacctg aaccaccgtg tgactgacga atcaccacgt
    18781 tatcaccagt ttgctgtttc cagtgggcgc tgaatgcctt gttgtactgt tcgtacaatt
    18841 cgcgcgttgg atcatatgaa acgttaagaa gctgaatatc ctttgccata acgttggttg
    18901 ccgccagcaa aaatgttaac cctacgcccc acttgttcat cgcccgactc tcttatgttg
    18961 tgttgtgatg agcaaagcgt gccagaaggt taaccaaaca ttaaagaata aaaaaagatt
    19021 ggctataact tgcgggtata tgttgggggt taaaaaggcg gaagaaatcc gcctcatatt
    19081 gctgacaaag tgcgctttgt ccatgccgga tgcggcgcga acgccttatc cggcctacaa
    19141 aagtttgcaa attcaataaa ttgcagaatt catgtaggcc tgataagcga agcgcatcag
    19201 gcatttttgc ttctgtcatc ggtttcaggg taaaggaatc tgcctttttc cgaaatcatt
    19261 aatacagttt tttcgcgcag tccagccagt cacctttgaa cggacgcttc atgttttcga
    19321 tagcgtcgat gatgtcgtgg tgaaccagct gttcgttctg gatacctaca caacgaccgc
    19381 cgtaacctgc cagcagcaga tcgatagcgt aagcgcccat acgggaagcc agaatacggt
    19441 cgtaaggcac cggagaacca ccgcgctgga tgtgtcccag cacagttgcg cgggtttcac
    19501 gaccggtttc tttctcgatg aaatgcgcca gttcgtcaac atcacacata tgttcggtaa
    19561 tcgccacgat cgcgtgtttt ttacctttcg cgatacccgc tttgatttcg tttaccaggt
    19621 cttcacggct gaattcaact tccggaacca caacgaattc acagccaccg gcaatggccg
    19681 cagccaacgt cagatcgcca caataacggc ccatcacttc caccacggaa atacgctggt
    19741 gagaagaaga ggtgtcacgc agacggtcga tcgcttctac aacggtgctc agcgcagtga
    19801 agaaaccgat agtgtagtca gtgcctttga tgtcgttgtc gatagtgccc ggcagaccga
    19861 tgcacgggaa gcccatttcg gtcagacgca ttgcacccat gtaggaaccg tcaccgccga
    19921 taaccaccag cgcgtcgatc ccacgttttt tcaggttttc gatagccacg gcgcggatgt
    19981 tctcgtcgcg gaattccggg aagcgcgcag aaccgaggaa cgtaccgcca cggttgatca
    20041 tgtcagaaac gctgtaacgg tctagctgta ccatacggtc ttcatacaga cccagatagc
    20101 cgtcataaat gcccattact tccagacctt ctgtcagcgc agaacgaaca accccgcgaa
    20161 ttgcggcgtt catgcctggc gcatcaccgc cgcttgtcaa cacaccgatt ttcttaatca
    20221 tgactacctc tgaactttgg aatgcaaaat aaaatctgtt gccggaagtc ttcttgcaca
    20281 tcgaagtgat ccaacgaatg tgcaaatagt ataacaatca cttcctgctg aattgattca
    20341 ggtcaggcca aatggcggta ttttatacac aaaatgcggg tctggctctc ttttatactg
    20401 attatgaaag catagaccgt ttaccctccc tgggtacgac ggaacagggg tcctgatgga
    20461 taattacatc cgatcccgga aaacgccgta aaatagcctg ctctacctga tccgccacca
    20521 tatgtgcctg aaccaaaggc agagagtctt ccatttccaa atgaatctga ataaagcggg
    20581 tcggccctga ctgccgcgtg cgaagatcgt gagcgccgct aacacccggc caggaagtca
    20641 cgatatcaat aatttcttgc cgttcctcat caggcaatgc gcgatccagt aatgactgta
    20701 ccgcctcata tcccatgcgt aacgcgctat ataaaatata gatgccgatt cccaatgcaa
    20761 acagagcatc ggcgcgatgc cagccgtacc aggacaaccc cagcgccagc agaattgcgc
    20821 cgttcatcat aacatcagac tggtaatgta gcatatcagc ccgcaccgcc tggctttgcg
    20881 tccggcgcac cacccaacgc tgaaacgaga caaggataat cgtacaaatt agcgccacaa
    20941 ttgtcacgat aaccccgacg cctggatctg tcatcggtgt tggagatatc agatgttgaa
    21001 tacccgtcaa aaacaggaat agtgccgaac cggagataaa catactttgc gccagcgccg
    21061 cgagggactc tgctttaccg tgaccaaacg agtgattatc gtcggcaggt tgcagggaat
    21121 atcgcaccac cagtaaattc gtcaacgacg cgccgatatc caccagcgaa tccaccagcg
    21181 cggcgagaat actcaccgac ccggtatacc accatgcaaa aattttaatc agcaatagca
    21241 acgaagccat cgccgtcgca gcaatcgccg cccgactgac cagccgtcca taagattgat
    21301 tcataaatac tcccgctatc aactgacgct agtataacgg aagcaaatca tctgcaatgc
    21361 attaagcagc aggcaaattg aggataaaaa aaaaccccac atcatgtggg ggaagacagg
    21421 gatggtgtct atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc
    21481 aatagcttca acgatgaact tttttgccat tgcgtcacgt cacgcaactg ctccattcgt
    21541 tgttgatgtt tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg
    21601 ttgcggactt ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc
    21661 tgtgcgcgca cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc
    21721 agttcgctaa cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc
    21781 tgctgacgct gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc
    21841 tgcgtaagtt cttcacccgg atgccagtta tcgcctgaac cgacttcagc agcgtggctt
    21901 aatgaactga ctgccagcgt tgaggccatg acggcagcgg taactatgcg catcatttgc
    21961 tcccaaaatc tttctgtcgc gattcaacga tagagagttt acgattcagg ctgcaaacat
    22021 gcgtcagggg gtgtaaaaca acgtaaagtc atggattagc gacgtctgat gacgtaattt
    22081 ctgcctcgga ggtatttaaa caatgaataa aatcctgtta gttgatgatg accgagagct
    22141 gacttcccta ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg ttgcccacga
    22201 tggggaacag gcgcttgatc ttctggacga cagcattgat ttacttttgc ttgacgtaat
    22261 gatgccgaag aaaaatggta tcgacacatt aaaagcactt cgccagacac accagacgcc
    22321 tgtcattatg ttgacggcgc gcggcagtga acttgatcgc gttctcggcc ttgagctggg
    22381 cgcagatgac tatctcccga aaccgtttaa tgatcgtgag ctggtggcac gtattcgcgc
    22441 gatcctgcgc cgttcgcact ggagcgagca acagcaaaac aacgacaacg gttcaccgac
    22501 actggaagtt gatgccttag tgctgaatcc aggccgtcag gaagccagtt tcgacgggca
    22561 aacgctggag ttaaccggta ctgagtttac cctgctctat ttgctggcac agcatctggg
    22621 tcaggtggtt tcccgtgaac atttaagcca ggaagtgttg ggcaaacgcc tgacgccttt
    22681 cgaccgcgct attgatatgc acatttccaa cctgcgtcgt aaactgccgg atcgtaaaga
    22741 tggtcacccg tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt ctgcttcatg
    22801 ataggcagct taaccgcgcg catcttcgcc atcttctggc tgacgctggc gctggtgttg
    22861 atgttggttt tgatgttacc caagctcgat tcacgccaga tgaccgagct tctggatagc
    22921 gaacagcgtc aggggctgat gattgagcag catgtcgaag cggagctggc gaacgatccg
    22981 cccaacgatt taatgtggtg gcggcgtctg ttccgggcga ttgataagtg ggcaccgcca
    23041 ggacagcgtt tgttattggt gaccaccgaa ggccgcgtga tcggcgctga acgcagcgaa
    23101 atgcagatca ttcgtaactt tattggtcag gccgataacg ccgatcatcc gcagaagaaa
    23161 aagtatggcc gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga agataattac
    23221 caactttatc tgattcgtcc ggccagcagt tctcaatccg atttcattaa cttactgttt
    23281 gaccgcccgc tattactgct gattgtcacc atgttggtca gtacgccgct gctgttgtgg
    23341 ttggcctgga gtctggcaaa accggcgcgt aagctgaaaa acgctgccga tgaagttgcc
    23401 cagggaaact tacgccagca cccggaactg gaagcggggc cacaggaatt ccttgccgca
    23461 ggtgccagtt ttaaccagat ggtcaccgcg ctggagcgca tgatgacctc tcagcagcgt
    23521 ctgctttctg atatctctca cgagctgcgc accccgctga cgcgtctgca actgggtacg
    23581 gcgttactgc gccgtcgtag cggtgaaagc aaggaactcg agcgtattga aaccgaagcg
    23641 caacgtctgg acagcatgat caacgatctg ttggtgatgt cacgtaatca gcaaaaaaac
    23701 gcgctggtta gcgaaaccat caaagccaac cagttgtgga gtgaagtgct ggataacgcg
    23761 gcgttcgaag ccgagcaaat gggcaagtcg ttgacagtta acttcccgcc tgggccgtgg
    23821 ccgctgtacg gcaatccgaa cgccctggaa agtgcgctgg aaaacattgt tcgtaatgct
    23881 ctgcgttatt cccatacgaa gattgaagtg ggctttgcgg tagataaaga cggtatcacc
    23941 attacggtgg acgacgatgg tcctggcgtt agcccggaag atcgcgaaca gattttccgt
    24001 ccgttctatc ggaccgatga agcacgcgat cgtgaatctg gcggtacagg attggggctg
    24061 gcgattgttg aaaccgccat tcagcagcat cgtggctggg tgaaggcaga agacagcccg
    24121 ctgggcggtt tacggctggt gatttggttg ccgctgtata agcggagtta aactccgcat
    24181 ttgtaggcag gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga
    24241 cgctgatagc gtcttatcag gcctacactc cgggggttat tttccccaca accggcggga
    24301 aaaatcttcg atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga
    24361 taacccggcg gcggagagta aacggtgata ctgatcgtca tcaaacggca tatgccatgc
    24421 gatggcggca gcctcctgca cggtcacatc gctgacacgg gaaaccaact ccagcggcgc
    24481 gtccgcagac actttccctg gcgctatcac gctatacaac caccccacct tacctgtgtt
    24541 ttgcatcaac tgcgctatat cgctgatatc aaaatggtaa ttgagtttat agcaaggcga
    24601 gcgcggctgg ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc ccatatgaac
    24661 attactttcc gtgagaccgt cggttgagag attttcgcca aacgcaggcg caacaaacaa
    24721 ctccgcctgt tccggaaatt cccgcaccca gtagagatag tgctcacgag gataatgaca
    24781 cagcgctctg tctggcccgc cgtgaacctt tttctccgcc tgctcgtcac cttccagccc
    24841 cagctccgtc agcatcaact caccatcaac ctggattttg gcaattgcgc tgggtttgcc
    24901 ttcgggataa gcctgaatct tgcctgtgta tacatcaacc ggatatcgca tctgttccct
    24961 ctccacgaga aatttcccca ttagaccata tttattttat gtcaggcgtg ctgttttcgc
    25021 ggtaagtatc caggcaggaa ctcaatagca aaccggacga acgttagttc atccggctgt
    25081 ggggattaag gaagcaggtt tatttcaccg tacctaaaat ttcgattttc gctgctctgg
    25141 ctttgacttt acgtgaccag atagaggtaa gaattggcac cagaatcgaa gtcacaatga
    25201 ccgccgtcgc taccagcgaa gttgctgccg gagccatcgg tttaaacgca ggcaccattt
    25261 ctgcaatcag cacaggtgtc gctaccgcgg cccctgcgga actggaagcg gcaattccgg
    25321 cagtgccatc gccaccgcca atcaatttat cggcgataat caacggaata ccggtcacga
    25381 taattactgc cacacccaac aggatcccca gcaaaccagt ctgggcaatc acagtcaaat
    25441 caatggtatt gcccagcgcg aaggcaaaga atggaatcag cgtttgcacc gctttgctga
    25501 aaaattctcg taattcaggg tcacggttcc ccagggcaaa gcccaccagg aacggtaata
    25561 ctgcgccgac gaaaacatgc ggttcaaacg aggcaatccc tgcagtgccc agaataatca
    25621 tcgtcatgag cggcccggac tccaacgaca tcaacacaaa tgccccagct tcttcttttg
    25681 tgccgtactg ctgcatgatg gaagcgtaaa gtccgccatt ggtcatatcc atcgccgcca
    25741 ccagcgccag cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa
    25801 tgcgcgaggc aattgccgca accacccacg cgacggcaat ttttgttacc accagcgtac
    25861 cggatttacg caataccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa
    25921 accacaccgc cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat
    25981 atttccccgc ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc gggacgagca
    26041 tcatcccccc cgggattttc tcaatcgagc gttttatctg catttccatc acctgtcggc
    26101 atatttttaa gggctgatgc gtcattcctc aatggagcat cagtgttgat ggaaacgaag
    26161 atagacattc atttaatcaa taaaagtgat caaaaccaca tcaattgaaa cgctgtttta
    26221 tttttatcgg atcattgttt ttattttgaa attgatcaca aaaaaaccac cgccgttggc
    26281 gatggttcat tttttaagct gatatgcggc ctatacgcct cattgcagca ggcggcaaat
    26341 gattattttt tcgccgcaaa acgtgccgct gcttcgtccc agttcaccac gttccagaac
    26401 tctttaatgt agtccggacg gcggttctgg aatttcaggt agtaagcatg ttcccacaca
    26461 tccaggccca gaatcgggaa gccggaagcg ccagaaatag cttcacccat cagcggagaa
    26521 tcctggttag cagtagaaac caccgccagt ttatcgcctt tcagcaccag ccatgcccag
    26581 ccggaaccaa agcgggaagc tgccgctttt tcaaattctg ctttgaagtt atcaacggag
    26641 ccgaagtcac gttcgatagc cgctttcagg tcaccctgca gggtggtgcc ttttttcaga
    26701 cctttccaga acaggctgtg gttagcgtga ccgccagcgt tgttgcgcag tacggttttc
    26761 ttgtctgctg gcagctggtc cagtttggtg atcagctctt caaccggcag gttggcaaat
    26821 tctggcaggc tttccagcgc cgcgttggcg ttgtttacgt aggtctgatg gtgtttggtg
    26881 tggtggattt ccatggtctg cttatcgaag tgcggttcca gggcatcgta agcatacggc
    26941 agggatggca gggtatagct catattcatc tccagtattg tcgggcggcc gattgttaat
    27001 gccgcgtaag cagttggttc attatagtta attaaatgat attgaaaatg attatcaatg
    27061 ccgtactttt cgtaagggta tggttttgca ggaaaatgcc cgagatgtga agcaaatcac
    27121 ccacttaatg ccgtgattgc cagtaaatcg acaacggcgg caacaggcga aaggttaatc
    27181 gacagcacga tttttacact catctcgtcg gagatgtgaa gcgacgaaaa atgatgagga
    27241 taagaagatg agtaacgcga ttacgatggg gatattttgg catttgatcg gcgcggccag
    27301 tgcagcctgt ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg
    27361 gtcagtcggt gggattgttt cgtggattat tctgccgtgg gccatcagcg ccctgttact
    27421 accggatttc tgggcgtatt acagctcgtt tagtctctct acgctgctgc ctgtttttct
    27481 gttcggcgct atgtggggga tcggtaatat caactacggc ctgaccatgc gttatctcgg
    27541 catgtcgatg ggaattggca tcgccattgg cattacgttg attgtcggta cactgatgac
    27601 gccaattatc aacggcaatt tcgatgtgct gattaacacc gaaggcggac gcatgacgtt
    27661 gctcggcgtt ctggtggcgc tgattggcgt agggattgta actcgagccg ggcagttgaa
    27721 agagcgcaag atgggcatta aagccgaaga gttcaatctg aaaaaagggc tggtgctggc
    27781 ggttatgtgc ggcattttct ctgccgggat gtcctttgcg atgaacgccg caaaaccgat
    27841 gcatgaagcc gctgccgcac ttggcgtcga tccactgtat gtcgctctgc caagctatgt
    27901 tgtcatcatg ggcggcggcg cgatcattaa cctcggtttc tgttttattc gtctggcaaa
    27961 agtgaaggat ttgtcgctaa aagccgactt ctcgctggca aaaccgctga tcattcacaa
    28021 tgtgttactc tcggcactgg gcgggttgat gtggtatctg caattctttt tctatgcctg
    28081 gggccacgcc cgcattccgg cgcagtatga ctacatcagt tggatgctgc atatgagttt
    28141 ctatgtattg tgcggcggta tcgtcggact ggtgctgaaa gagtggagca atgcaggacg
    28201 ccgtccggta acggtgttga gcctcggttg tgtggtgatt attgtcgccg ctaacatcgt
    28261 cggcatcggc atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt
    28321 catcccggtt tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc
    28381 ggtcgaaata tcactgatta acaggcggct atgctggaga agatattgcg catgacacac
    28441 tctgacctgt cgcagatatt gattgatggt cattccagtc tgctggcgaa attgctgacg
    28501 caaaacgcgc tcactgcacg atgcctcatc acaaaattta tccagcgcaa agggactttt
    28561 caggctagcc gccagccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg
    28621 caacgaatca ctggtgtaac gatggcgatt cagcaacatc gccaactgcc cgaacagcaa
    28681 ctctgccatt tcgttagcaa acggcacatg ctgactactt tcatgctcaa gctgaccgat
    28741 aacctgccgc gcctgcgcca tccccacgct acctaagcgc cagtgtggtt gccctgcgct
    28801 ggcgctaaat cccggaatcg ccccctgcca gtcaagattc agcttcagcc gctccgggca
    28861 ataaataata ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc
    28921 atgaatgtaa aagagatcgc cacgggtaat gcgataaggg cgatcgttga gtacatgcag
    28981 gccattaccg cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cagcaaagac
    29041 atgttgcgga taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc
    29101 tttgagaagt tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt
    29161 attcgcaata tggcgtacaa atacgttgag aagattcgcg ttattgcaga aaaccatccc
    29221 gtccctgacg aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt
    29281 ggttactgtc gctgaatccg caatgatagg cgatgtcagt aacgctggcc tcactgtggc
    29341 gtagcagatg tcgggccttc atcagtcgca ggcggttcag gtaacgctga ggcgtcagtc
    29401 ccgtttgctg cttaagctgc cgatgtagcg ttcgcagtga aagagaaaat tgatccgcca
    29461 cggcatccca attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga
    29521 gacgtgatgc gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta
    29581 attgcataaa caagatctcg cgactggcgg tcgagggtaa atcattttcc ccttcctgct
    29641 gttccatctg tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta acgcgccagt
    29701 gagacggata ctgcccatcc agctcttgtg gcagcaactg attcagcccg gcgagaaact
    29761 gaaatcgatc cggcgagcga tacagcacat tggtcagaca cagattatcg gtatgttcat
    29821 acagatgccg atcatgatcg cgtacgaaac agaccgtgcc accggtgatg gtatagggct
    29881 gcccattaaa cacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat
    29941 gatgatgttc aggaaaatcc gcctgaggga gccggggttc tatcgccacg gacgcgttac
    30001 cagacggaaa aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac
    30061 acggcgaaat agtaatcacg aggtcaggtt cttaccttaa attttcgacg gaaaaccacg
    30121 taaaaaacgt cgatttttca agatacagcg tgaattttca ggaaatgcgg tgagcatcac
    30181 atcaccacaa tgaagcaaat tgtgaacatc atcacgttca tctttccctg gttgccaatg
    30241 gcccattttc ctgtcagtaa cgagaaggtc acgaattcag gcgcttttta gactggtcgt
    30301 aatgaaattc agcaggatca cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc
    30361 gcatccagtg ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg
    30421 cgcgaaatcc atcgttttaa aaatgggctg catagccaga acggttatgt cacctgggat
    30481 gtggatagcc tggaaagtgc cattcgcctt ggcttaaaca aagtgtgtga ggaagggatt
    30541 cgtatcgata gcattgggat tgatacctgg ggcgtggact ttgtgctgct cgaccaacag
    30601 ggtcagcgtg tgggcctgcc cgttgcttat cgcgatagcc gcaccaatgg cctaatggcg
    30661 caggcacagc aacaactcgg caaacgcgat atttatcaac gtagcggcat ccagtttctg
    30721 cccttcaata cgctttatca gttgcgtgcg ctgacggagc aacaacctga acttattcca
    30781 cacattgctc acgctctgct gatgccggat tacttcagtt atcgcctgac cggcaagatg
    30841 aactgggagt acaccaatgc caccaccaca caactggtca atatcaatag cgacgactgg
    30901 gacgagtcgc tactggcgtg gagcggggcc aacaaagcct ggtttggtcg cccgacgcat
    30961 ccgggtaatg tcattgggca ctggatttgc ccgcagggta atgagattcc ggtggtcgcc
    31021 gttgccagcc atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc
    31081 gcttatctct cttctggcac ctggtcattg atgggcttcg aaagccagac gccatttacc
    31141 aatgacacgg cgctggcagc caacatcacc aatgaaggcg gggcggaagg tcgctatcgg
    31201 gtgctgaaaa atattatggg cttatggctg cttcagcgag tgcttcagga acggcaaatc
    31261 aacgatctcc cggcgcttat cgccgcgaca caggcacttc cggcctgccg cttcatcatc
    31321 aatcccaatg acgatcgctt tattaatcct gaggcgatgt gcagcgaaat tcaggctgcg
    31381 tgtcgggaaa cggcgcaacc gatcccggaa agtgatgctg aactggcgcg ctgtattttc
    31441 gacagtctgg cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct gcgcggtgaa
    31501 gatttctcgc aactgcatat tgtcggcggc ggctgccaga acacgctgct caaccagcta
    31561 tgcgccgatg cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc aacgctcggc
    31621 aatatcggca tccagttaat gacgctggat gaactcaaca atgtggataa tttccgtcag
    31681 gtcgtcagca ccaccgcgaa tctgaccacc tttaccccta atcctgacag tgaaattgcc
    31741 cactatgtgg cgcagattca ctctacacga cagacaaagg agctttgcgc atgaccactc
    31801 aactggaaca ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg
    31861 aggaggcgct gcgccaactt gatcgtttac ccgtttcaat gcactgctgg cagggcgatg
    31921 atgtttccgg ttttgaaaac ccggaaggtt cgctgaccgg tgggattcaa gctactggta
    31981 attatccggg caaagcgcgt aatgccagtg agctacgtac cgatctggaa caggctatgc
    32041 ggctgattcc ggggccgaaa cggcttaatt tacatgccat ctatctggaa tcagacacgc
    32101 cagtctcgcg cgaccagatc aaacctgagc acttcaaaaa ctgggttgaa tgggcgaaag
    32161 ccaatcagct cggtctggat tttaacccct cctgtttttc gcatccgcta agcgccgatg
    32221 gctttacgct ttcccatgcc gacgacagca ttcgccagtt ctggattgat cactgcaaag
    32281 ccagccgtcg cgtttcggcc tattttggcg agcaactcgg cacaccatcg gtgatgaaca
    32341 tctggatccc ggatggtatg aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc
    32401 tgctggcagc actggatgag gtgatcagcg agaagctaaa ccctgcgcac catatcgacg
    32461 ccgttgagag caaattgttt ggcattggcg cggagagcta cacggttggc tccaatgagt
    32521 tttacctggg gtatgccacc agccgccaga cggcgctgtg cctggacgcc gggcacttcc
    32581 acccgactga agtgatttcc gacaagattt ccgccgccat gctgtatgtg ccgcagttgc
    32641 tgctgcacgt cagtcgtccg gttcgctggg acagcgatca cgtagtgctg ctggatgatg
    32701 aaacccaggc aattgccagt gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg
    32761 gccttgactt cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt ggtacacgca
    32821 atatgaaaaa agccctgctg cgtgcgttgc tggaacctac cgccgagctg cgcaagttgg
    32881 aagcggcggg cgattacact gcgcgtctgg cactgctgga agagcagaaa tcgttgccgt
    32941 ggcaggcggt atgggaaatg tattgtcagc gtcacgatac cccagcgggt agcgaatggc
    33001 tggagagcgt gcgggcttat gagaaagaaa ttttgagtca gcgtgggtaa acactgccag
    33061 atgcggcacg agcgccttat ccggcctaca gatcgtgcat ctgacaacac cccatttaca
    33121 ggaacaccga acatgcaaaa cattactcag tcctggtttg tccagggaat gatcaaagcc
    33181 accaccgacg cctggctgaa aggctgggac gagcgcaacg gcggcaacct gacgctacgc
    33241 ctggatgacg ctgacatcgc accgtatcac gacaatttcc accaacaacc gcgttatatc
    33301 ccgctcagcc aacccatgcc tttactggca aatacaccgt ttatcgtcac cggctcgggc
    33361 aaattcttcc gtaacgtcca gcttgatcct gcggctaact taggcgtcgt aaaagtcgac
    33421 agcgacggcg cgggctacca tattctctgg gggttaacca acgaggccgt tccaacttct
    33481 gaacttccgg cacactttct ctcccattgc gaacgcatta aagccaccaa cggtaaagat
    33541 cgggtgatca tgcactgcca tgccaccaac ctgatcgccc tcacctatgt actggaaaac
    33601 gacacggcgg tcttcactcg ccaactgtgg gaaggcagca ccgagtgcct ggtggtattc
    33661 ccggatggcg ttggcattct gccgtggatg gtgcccggca cggacgaaat cggtcaggcg
    33721 accgcacaag aaatgcaaaa acattcgctg gtgttgtggc ccttccacgg tgtgttcggt
    33781 tccggaccga cactggatga agcctttggt ttgatagata ccgcagagaa atcagcggag
    33841 gtgttagtca aaatctactc gatgggcggt atgaagcaga ccattacccg tgaggagctg
    33901 attgcactcg gtgagcgttt tggcgttacg ccactggcca gtgcgctggc actttaatat
    33961 cccaacagat atccggcggt gtgggaaacg tcgccggaca gatggatttg aacttaagga
    34021 gagtcattat gagttttatg ttggcattac cgaaaattag cctgcatggt gctggggcaa
    34081 ttggcgatat ggtaaatctg gttgcgcata aacagtgggg aaaagcactg atcgttaccg
    34141 atgggcaact ggttaagttg ggattactgg acagcctgtt tatcgcactg gatcaatacg
    34201 aaatgtctta tcacttattt gatgaggtat ttcctaaccc cactgaagaa ctggtgcaaa
    34261 aaggctacgc tgcatttcag gatgccgcat gtgactacat tattgctttc ggtggcgata
    34321 gcccaattga tactgcaaaa gccgtaaaaa tccttaccgc taatcctgcg ccttctacgg
    34381 cgtactctgg tgttggtaaa gtgacaaatc ccggcgtacc gctggtggca atcaatacga
    34441 ctgccggaac agctgctgag atgaccagca atgcggtgat catcgacacc gcgcggcagg
    34501 taaaagaggt cattatcgac ccgaatatta ttcccgatat tgcggtggat gatgccagtg
    34561 taatgctgga gatcccggcc tcggttaccg ccgccacggg tatggatgca ctgactcacg
    34621 cagttgaagc ttatgtttct gttggtgcac atccattaac cgacgctaac gcactggaag
    34681 ctatccgttt aattacgctg tggctgccaa aagcggtaga tgatggtcat aatctggaag
    34741 ctcgtgaaca aatggcattc gcccagtacc tggcgggtat ggcatttaac agtgctggtc
    34801 tgggccttgt tcatgcactt gcgcatcagc ccggcgccac ccacaacctg ccgcatggtg
    34861 tgtgcaatgc tatcttgttg ccagttattg aaaactttaa ccgcccgaat gccatcgcac
    34921 gctttgcccg cattgctcag gcaatgggtg tcgatacgcg tgatatgagt gaagaagcgg
    34981 caagtactga agcaattaac gctatccgcg cattaagcca tcgcgtgggt attccggcag
    35041 gattcagcca gcttggcatc accaaagaag atattgaagg ctggttagat aaagccctgg
    35101 ctgacccgtg tgtgccatgt aacccgcgta ccgccagccg cgacgaggtg cgcgagctgt
    35161 atctggaggc attatgatcc gtaaggcatt tgtgatgcag gttaaccccg acgctcacga
    35221 agagtatcaa cgtcggcata accccatctg gccggaactg gaagcagtgt tgaaatctca
    35281 cggtgcgcat aactacgcca tttacctcga caaagcgcgt aatctgctgt ttgccacggt
    35341 agagattgaa tctgaagagc gctggaatgc ggttgccagt actgatgttt gccaacgttg
    35401 gtggaaatat atgaccgacg tcatgcccgc gaacccggat aacagcccgg tgagtagcga
    35461 actgcaagaa gtgttttacc tgccgtaatt gcctaatgtg ccggagacgc ctccggtaca
    35521 atatttttaa tttcgatttg aaaaataaac atattacacc ttcagttaat atatctcggt
    35581 aacttatatt tccccgatgc attgtgagta aatcacaaaa ataatgaata actcattaat
    35641 gattcatgtg gtttatttaa ataaccatta tgtgcattac tccgcaaatc tggcctttca
    35701 ctctgtctca aattgtcatt gtttaccgac atatcacctc tcatcactta atcgctccac
    35761 cattacaatt gattaaaata tattttatgg agtccgatta atggcagctc ttactgcaag
    35821 ctgtattgac ctgaatattc agggcaatgg cgcttattcc attctgaagc agttggcgac
    35881 aatggcgtta caaaacggtt ttatcaccga ctcacaccag ttcctgcaaa ccctgctgct
    35941 gcgcgaaaaa atgcactcta ctggatttgg ttccggcatc gccgtgccgc acggtaaaag
    36001 cgcctgcgtt aaacaaccgt tcgtgttatt cgcccgcaaa gcgcaggcca ttgactggaa
    36061 agccagcgac ggcgaagacg tcaattgctg gatctgcctc ggcgtgccgc aaagtggcga
    36121 agaggatcag gtcaaaatca tcggcacact gtgtcgcaaa attatccatc aggattttat
    36181 caaccaactg cgacagggcg acgccgacca ggtacttgct ttgttaaatc aaaccctcag
    36241 ctcataagga agtggcgatg gagtcatcct tacgtattgt cgcgatcacc aactgccccg
    36301 ccgggatcgc tcacacctac atggtggcgg aagccctgga acagaaagcc cgttctctcg
    36361 gccataccat aaaagtggaa actcaagggt ccagcggcgt tgaaaaccgc ttatccagcg
    36421 aagagattgc cgctgccgat tacgtcattc tagctaccgg gcgtggcctg agcggtgatg
    36481 accgggcgcg gtttgccggg aagaaagttt atgagattgc catctcccag gcgctgaaaa
    36541 atatcgacca gattttcagc gaattaccaa caaactcgca gctttttgcc gcagatagcg
    36601 gcgtgaagct cggcaaacag gaaatgcaaa gcggcagcgt gatgagccac ctgatggcgg
    36661 gcgtctctgc ggcgctgcca tttgtcatcg gcggcggtat tctggtggcg cttgccaaca
    36721 tgctggtgca gttcggtttg ccatataccg atatgtcgaa aggtgcgccg tcgtttacct
    36781 gggtagttga atccatcggt tacctcggct tcacctttat gatccccatc atgggcgctt
    36841 atattgcctc gtcgattgcc gataaacccg ctttcgctcc ggcatttctg gtttgctatc
    36901 tggcgaacga taaagccctg ctcggcaccc agtcaggcgc gggcttcctc ggcgcggtgg
    36961 tgctggggct ggcgattggc tatttcgtct tctggttccg taaagtccgt cttggcaaag
    37021 cattacaacc gctgctcggc tccatgctta tcccattcgt tactctgctg gtttttggcg
    37081 tgctcactta ctacgttatc gggccggtga tgtccgacct catgggcggg ctgctccact
    37141 tcctgaatac cattcctcca tcaatgaagt ttgcggcagc gtttctggtc ggtgcgatgc
    37201 tggcgttcga tatgggcggc cccatcaata aaaccgcctg gttcttctgc ttctcactgc
    37261 tggaaaaaca catttatgac tggtacgcca tcatcggcgt tgtcgcgctg atgccgcccg
    37321 tcgcggcggg tcttgcgacg ttcattgcgc ccaaactgtt tacccgacag gaaaaagagg
    37381 ctgccagtag tgccattgtg gtcggtgcca cagtcgcaac cgaaccggct attccttacg
    37441 ctctggcagc tccgttgccg atgattaccg ccaatacgct ggcgggcggt attaccggcg
    37501 tgctggtgat cgctttcgga atcaaacgtc tggcacctgg tttgggtatc tttgacccgc
    37561 tgatcggcct gatgtcaccg gtaggttcgt tttatctggt gctggcgatt ggcctcgcgc
    37621 tgaacatttc attcattatc gtcctgaaag gattgtggtt acgccgcaaa gcgaaagccg
    37681 cgcaacagga gcttgtccat gaacattgag ttactgcaac agttgtgcga agccagcgcc
    37741 gttagcggcg atgaacagga agttcgcgac attctgataa acacgctgga accttgcgtg
    37801 aatgaaatca cctttgatgg gctgggcagc tttgtcgccc gtaagggaaa taaagggccg
    37861 aaagttgccg ttgtcgggca tatggatgaa gtcggcttta tggtcaccca catcgacgag
    37921 agcggttttc tgcgtttcac caccattggt ggctggtgga atcagtcgat gctcaaccac
    37981 cgggtaacca tacgcacaca caagggagtg aaaatccctg gtgtgattgg ttccgtcgcg
    38041 cctcatgcgt taacggaaaa gcaaaagcaa cagccgcagt catttgatga gatgttcatt
    38101 gatattggcg caaacagtcg cgatgaggtg gaaaaacgcg gtgtggcgat gggcgatttt
    38161 attagcccgg aagccaattt tgcctgctgg ggcgaagata aagtggtcgg caaggcgttg
    38221 gataaccgca tcggctgcgc aatgatggct gagctactac agacggtgaa taatcccgaa
    38281 attacgctgt atggcgttgg cagtgtggaa gaagaagttg ggctacgcgg ggcgcaaacc
    38341 tcggctgaac acattaaacc ggatgtggtg atcgtgctgg acaccgctgt cgcgggcgat
    38401 gttccaggca ttgataacat taaatatccg ctgaaactgg gcaacgggcc ggggttgatg
    38461 ctgtttgaca agcgctactt ccccaaccag aaactggtag cagcgttaaa aagctgtgcc
    38521 acgcagaatg atttaccgct gcaattttcc accatgaaaa ccggtgcgac ggatggcggg
    38581 cgctacaacg tgatgggcgg tgggcgtccg gttgtcgcgc tgtgtttgcc aacgcgttat
    38641 ctgcacgcca acagcggcat gatttcaaaa gccgattacg atgctctgct cacgctgata
    38701 cggggttttc tgaccacctt aaccgcggaa aaagtcagcg cgtttagcca gttccgtcag
    38761 gtggattaat gctaaacgaa cgccagttaa agattgtcga tctgctggag caacagccgc
    38821 gcacgcctgg cgagctggcg caacagactg gcgtttctgg caggaccata ctgcgtgata
    38881 ttgactatct caactttacc cttaacggca aagcccgcat ttctgccagt ggtagtgcgg
    38941 gctatcagct ggaaatcttc gagcgccgca gcttttttca gttgctgcaa aagcacgata
    39001 acgacgatcg gctgctggca ctgttattac tgaatacctt tactccccgt gcgcaactcg
    39061 cctcggcgct taatttgccg gaaacgtggg tcgcagagcg tctgccccgg ttaaaacagc
    39121 gttatgaacg cacttgctgc ctggccagtc gccccggttt gggccatttc attgatgaga
    39181 cagaagaaaa acgcgttata ttgctggcga acttgctgcg caaagatccg tttttaattc
    39241 cgctggcggg cgtaacacga gacaaccttc agcatttatc cacggcctgc gacaaccaac
    39301 accgctggcc gctcatgcag ggtgattatc tctccagcct gattctggcg atttacgccc
    39361 tgcgtaatca actgaccgat gagtggccgc aatatcccgg tgacgagata aaacaaatcg
    39421 ttgaacatag cggtctgttt cttggtgata acgctgtaag aaccctgaca ggtttgatag
    39481 agaaacagca tcagcaagcg cagataattt ctgccgataa tgtgcagcgg ttgctgcaaa
    39541 gggtgccggg catcgcgtca ttgaatatta ttgatacgca gctggttgag aacattaccg
    39601 ggcatttatt acgttgcctt gccgcaccgg tgtggattgc tgagcaccgc caaagcagca
    39661 tgaataacct gaaagccgcc tggcctgcgg cctttgatat gagtctgcac tttattacgc
    39721 tactgcgtga acagctcgat attccccttt tcgacagcga tctgatcggt ttgtattttg
    39781 cctgtgcgct ggaacggcat caaaacgaac gccagccgat cattttgctc tcggaccaga
    39841 acgcgattgc cactattaat cagctcgcca ttgagcgcga tgttttacat tgtcgggtga
    39901 ttattgcccg tagcttaagc gaacttgttg ccattcgcga agagattgag ccgttattga
    39961 tcattaacaa cagccattat ttactggatg acgcggtaaa taattacatc accgtaaaaa
    40021 atatcattac ggctgccggt atcgaacaaa taaaacattt tctggcgacg gcatttattc
    40081 gccaacagcc ggagcgtttt ttctctgctc ccggaagttt tcattattcg aatgtacgcg
    40141 gtgaaagctg gcaacatatt acccggcaaa tttgtgcgca attagtcgca caacaccata
    40201 ttaccgccga tgaagcacaa cgcatcatcg cccgcgaagg cgaaggtgaa aacctgattg
    40261 ttaatcgcct cgccatccca cattgctgga gcgaacagga gcgacgtttt cgtggatttt
    40321 ttattaccct cgcccaacca gttgaggtga ataacgaagt cattaaccat gtcttgatcg
    40381 cctgcgccgc cgccgatgcg cgtcatgagc taaaaatatt tagctatctg gcaagcatat
    40441 tgtgtcagca tccggcggag attattgccg ggttaacagg atatgaggca tttatggagt
    40501 tacttcacaa ggggtgaact gtgtcggatg cgccgcatcc gacactgcat ttatcatcaa
    40561 aaacgtttaa cgcaggctat tatagtcatc aaccatttcg ttgtactcgc gtaatgctct
    40621 cgggaaagag tcttcgacca tccagcttga gtttgcgtcc tgaagttgct ctttatccca
    40681 gtcagagtac gggactttat ctctgatgcg gcgaacgtat tttttagcct cgagttgata
    40741 ctggcctgcc gaattaataa acgagaaatt catgccgcct ttatccgctt ctttggcctg
    40801 cgccactagc gtttccagtt cagacacttt cttcattgcc gcatcaacat caaacttctc
    40861 ttgcgatatc aggttattaa tttgtttcgc tgaaatcatg actgccagag agtagtagtg
    40921 gaatgttttc ccttctcttt cttcaatatt tttcagttcc gcaagctggc gcttatcgtt
    40981 aatttcctga atcgccgcat gataagattc tgctacaggt tcaaaggctt ccagattttt
    41041 taagaatgtc tggtgcagcg ttttcccttt cgcaaacgca tcgtctttat aattttcctg
    41101 ggtgtaatat ttatccattt cgttaatggt attacccaac gccacggcag catcgatata
    41161 actcaccgcc acgctatcaa ttggttgcag cgcaggcgtt aacgccacag cactttttac
    41221 gcctttttca cactcagcga ggttggattc actaatgccg tagatgccat aaacagtacg
    41281 ctcttcaccg gtaggcccct gtttaaaatc tttcagccag tcagcataac gcgccagact
    41341 gctctgtaca gggatctgta acttgttata gcacttgata tagacatcca ttttttcgtc
    41401 gaaaaccgct tgttcatcga ccaccggttg cgcaggtgtt tcaggtttag cttctgcttt
    41461 tgcaacggga gcttcaggag ctggtgcggc aggttgacta ttggcaggcg ggggcgtttc
    41521 tgtttcggct tttttatcat cacaacccgt cagaccgagg gccatgatgg cgactataat
    41581 agcggaagat aataaattcc ttttcattta attgccttat aaatatatag ataagagagt
    41641 gaacataata aatgaaaaaa gaaaacgcca ctacacgcgt tttatttata aataaaataa
    41701 taaaattcac gaaatgaata agtcctgcaa tttaatatat atcgcaggac ttattttaat
    41761 taattgctca aacgctgcgg atgggtataa accgttgccc gacccggctt acaaaaaccc
    41821 accagcgtca gattacaacg ctcagccact tctacagcaa gcgtggtcgc ggcagacacc
    41881 gcaaacaaaa tctctacgcc gcacatcgcc gacttttgca ccatttcata actggcacga
    41941 ctggaaacca gtaccgcacc ttgctgccag ctttcccctt cttgtgaccg gcggcctaac
    42001 agtttgtcca acgccacatg gcgacccacg tcttcatgcc cgccgaccag ttcgccagat
    42061 ggcaacatcc aggcagcggc gtgagtacaa ccagtcagtt gccccactgg ctgaaaatcg
    42121 ttgagatgac gtaatgcatc atccagtttg ttgagatcaa acgtctgggt gaacggtagc
    42181 ggctgcaccg gttttccgat gtcattaagt tgctccacgc cgcatacgcc acagcccgta
    42241 cgtcccgcca gcgcccggcg gcgctccttc aaccccataa agcggcggct ggaaagctca
    42301 atttgtactt caagaccatt acaggaagga acgacatcca tgccgaagat atcgcgcgga
    42361 ctttctatta tcccctcgga aagcgaaaaa ccgagcgcaa agtactcaag gtctttgggc
    42421 gacgccatca tcaccacatg cgaaatgccg ttgtagacca gcgcaacggg aacttcttcc
    42481 gcgacctcat ctaaccgtgg gtgttgtaaa tcatcacgcc gccataactc aatttgacga
    42541 acacctgtga tatttgtcac attttcgatt tctttttgct gtgttttttt cactttctct
    42601 taaccagata agaacacaca caccaacatt atggtattct gttacaaacc cttcctggat
    42661 ggagggaaat tgagccaatt ctggaccttt gcggcccctt ccgcaaagaa aaataactcc
    42721 cactccctgc acacgcagca agcgaatgta aatgggacgt gacaatgtcg aaacaaggag
    42781 caatccatgc aggtcagcag aaggcagttc tttaagatct gcgctggcgg tatggcaggc
    42841 accacggcag cggcactggg ttttgcaccc agcgtagcgc tcgcggaaac ccggcagtat
    42901 aaactgctgc gcacccgcga aacccgtaat acctgcacct attgttccgt aggctgtggg
    42961 ctgttgatgt acagcctcgg tgacggagca aaaaacgcca aagcatctat cttccatatc
    43021 gaaggcgatc cggatcaccc ggtcaaccgc ggtgcacttt gtccgaaagg cgctggcctg
    43081 gtggatttca tccactccga aagccgtctg aagtttccgg aataccgtgc gccaggttct
    43141 gataaatggc agcaaatcag ttgggaagag gcgtttgatc gcatcgccaa actgatgaaa
    43201 gaagaccgcg atgctaacta cattgcgcaa aacgccgaag gcgtgactgt taaccgctgg
    43261 ctctccaccg ggatgctgtg tgcttccgcg tcgagtaacg aaaccggcta tttaacgcaa
    43321 aaattctccc gcgcgctggg tatgctcgcg gtcgacaacc aggcgcgtgt ctgacacgga
    43381 ccaacggtag caagtcttgc tccaacattt ggtcgcggtg cgatgaccaa ccactgggtc
    43441 gacatcaaga acgccaacct cgtcgtggtg atgggcggta acgccgctga agctcacccg
    43501 gtcgggttcc gctgggcgat ggaagccaaa attcacaacg gcgcgaagct gattgtgatc
    43561 gatcctcgct ttacgcgtac ggcggcggta gctgactact atgcccctat tcgttccggt
    43621 actgacattg ctttcctgtc aggcgtattg ctgtacctgc tgaacaatga aaaattcaac
    43681 cgcgaataca ccgaagccta taccaacgcc agcctgatag tgcgtgagga ttacggcttt
    43741 gaagatggcc tgttcaccgg ctacgacgcg gaaaaacgca agtacgataa atcctcctgg
    43801 acttatgaac tggacgaaaa cggcttcgcc aaacgcgata ccacgctgca acatccgcgc
    43861 tgcgtgtgga acttgctgaa acagcacgtt tcccgctaca cgccagatgt ggttgaaaac
    43921 atctgtggta cgccaaaaga cgcgttcctg aaagtctgcg aatacatcgc agaaaccagt
    43981 gctcacgata aaactgcctc gttcctgtat gccctcggct ggacgcaaca ctccgttggt
    44041 gcgcaaaaca ttcgtacgat ggcgatgatc cagctgctgc tcggcaatat ggggatggcc
    44101 ggcggcggcg ttaacgccct gcgcggtcac tccaatattc aggggctgac ggacctgggg
    44161 ctactgtcgc agagtctgcc aggttacatg acgctgccaa gcgagaagca gaccgatctg
    44221 caaacctacc ttaccgccaa cacgccaaaa ccgctgctgg aaggccaggt aaactactgg
    44281 ggcaactacc cgaaattctt cgtctctatg atgaaggcct tctttggtga taaagcgacg
    44341 gcggaaaata gctggggctt tgactggttg ccgaagtggg ataaaggcta cgacgtcctg
    44401 caatacttcg agatgatgaa agaaggcaag gtcaatggct atatctgcca gggctttaac
    44461 cctgttgcct cattcccgaa caaaaacaaa gtgatcggtt gtctgtcgaa actgaagttc
    44521 ctcgtcacca tcgacccgct gaacactgaa acctctaact tctggcagaa ccacggtgag
    44581 ctgaacgaag ttgactcgtc gaagatccag accgaagtgt tccgtctgcc atcgacctgc
    44641 ttcgcagaag agaacggttc aatcgttaac tcaggtcgct ggttgcagtg gcactggaaa
    44701 ggtgcggacg ccccggggat tgcgctgact gacggtgaga tcctctccgg tatcttcctg
    44761 cgcttgcgca agatgtatgc cgaacagggt ggcgcgaacc cggatcaggt gctgaacatg
    44821 acctggaact acgccattcc gcatgagccg aagtcggaag aagtagcgat ggagagcaac
    44881 ggtaaggcgc tggccgatat taccgatccg gcaaccggtg cggttatcgt caagaaaggc
    44941 caacaactta gctcgttcgc ccaattgcgc gatgacggta caacctcctg tggctgctgg
    45001 attttcgccg gtagctggac gccggaaggc aaccagatgg cgcgtcgtga taacgccgat
    45061 ccgtctggcc tcggtaacac gctgggctgg gcatgggcat ggccgcttaa ccgccgcatt
    45121 ctgtataacc gcgcctccgc agatccgcag ggtaacccgt gggatccgaa gcgtcagttg
    45181 ctgaaatggg acggcactaa gtggaccggc tgggatattc cggactacag cgcagcgcct
    45241 ccgggtagcg gcgtcgggcc gtttatcatg cagcaggaag gcatggggcg tctgtttgcc
    45301 ctcgataaga tggcggaagg tccgttcccg gaacactacg agccgtttga aacgccgctg
    45361 ggaactaacc cgctgcatcc aaacgttatc tcgaacccgg ctgcacgtat ctttaaagac
    45421 gacgccgaag cattgggtaa agccgataag ttcccgtatg tcggaaccac ctatcgtctg
    45481 accgagcatt tccactactg gaccaaacac gcgctgttga acgcgatttt gcaaccagag
    45541 cagtttgtgg aaatcgggga gtcgctggcg aataaacttg gcattgccca gggcgatacc
    45601 gtgaaagtct cctccaaccg tggctatatc aaggccaaag cggtggtgac caaacgtatt
    45661 cgtacactga aagcgaacgg caaagatatc gataccatcg gtattccgat tcactggggc
    45721 tatgaaggcg tagctaaaaa aggctttatt gccaatacgt taacgccatt cgttggtgat
    45781 gcgaacacgc agacgccgga gtttaagtcc ttccttgtga atgtggaaaa ggtgtaacgg
    45841 agacgacata tggcttatca atctcaagat atcattcgtc gttccgcgac taacggtctg
    45901 acccccgcgc ctgaggcgcg ggacttccag gaagaagtgg cgaaactcat cgacgtcacc
    45961 acctgtatcg gctgtaaagc ctgtcaggtg gcgtgttcag agtggaacga cattcgcgat
    46021 accgtcggca ataacattgg ggtgtacgac aaccccaatg atttaagcgc caaatcgtgg
    46081 acggtaatgc gcttctcgga agtggagcag aacgacaaac tggaatggct gatccgcaaa
    46141 gacggctgta tgcactgttc cgatccaggc tgcctgaaag cgtgcccggc ggaaggtgca
    46201 attattcagt atgccaacgg tatcgtcgac ttccagtccg agcagtgcat tggctgcggt
    46261 tattgcattg cgggctgtcc gttcgacatt ccgcgcctca acccggaaga caaccgcgtc
    46321 tacaaatgta cgctgtgcgt tgaccgcgtg gtggttgggc aagaaccagc ctgcgtgaag
    46381 acctgcccaa cgggcgcgat tcacttcggt acgaaagagt cgatgaaaac gctggcgagc
    46441 gagcgcgttg ctgagctgaa aacccgcggt tacgacaatg cgggtctgta cgatccggca
    46501 ggcgtcggtg gtacacacgt catgtacgtg ctgcaccatg ctgacaagcc aaatctgtat
    46561 catggcttgc cggagaaccc ggaaatcagc gaaaccgtga aattctggaa aggcatctgg
    46621 aaaccgctcg cggctgttgg ctttgcggct acctttgcgg ccagtatctt ccactacgtg
    46681 ggtgtcggtc cgaaccgtgc ggatgaggaa gagaataatc tgcacgaaga gaaagacgag
    46741 gagcgcaaat gaaacgacgt gacaccatcc tgcgctacac cgcgccggaa cgtatcaacc
    46801 actggatcac cgccttctgc ttcatcctgg cggcggtgag cgggctgggc tttttgttcc
    46861 cgtccttcaa ctggttgatg caaatcatgg gcacaccgca gctggcgcga attctgcacc
    46921 cgtttgtcgg cgtggttatg tttgcctcgt tcatcatcat gtttttccgt tactggcatc
    46981 acaacctaat caatcgggat gatatctttt gggcgaagaa tattcgtaag atcgtcgtca
    47041 acgaggaagt aggtgacacc gggcgttata acttcggtca gaaatgcgtt ttctgggcgg
    47101 cgattatttt cctggttctg ctgctggtga gcggcgtgat tatctggcgt ccttattttg
    47161 cgcctgcttt ctcaatcccg gtgatccgat tcgcgttaat gctgcattca tttgccgcag
    47221 tggcgttaat tgtggttatc atggtgcata tctacgccgc cctttgggtg aaaggcacga
    47281 ttaccgccat ggtggaagga tgggttacca gcgcctgggc gaagaaacat cacccgcgct
    47341 ggtaccgtga ggtccgcaag acaacggaaa agaaagctga atgagtattc gcataatccc
    47401 gcaagatgag ctgggttcga gcgagaaacg tacggcggat atgattccgc cgttattgtt
    47461 ccctcggctc aagaatttat acaaccgccg cgccgagcgt ctgcgcgagc tggcagaaaa
    47521 taatccgctg ggtgattacc tgcgctttgc tgcgcttatc gcccacgccc aggaagtggt
    47581 gctgtacgac catccgctgg agatggatct gaccgcgcgc attaaagaag ccagcgcaca
    47641 gggcaagccc ccgctggata ttcacgttct gccgcgtgat aagcactggc aaaagctgct
    47701 gatggcgctg attgctgagc tgaaacctga aatgagcggc ccggcgctgg cagtgattga
    47761 gaatctggag aaggcatcga ctcaggagct ggaagatatg gccagcgcac tgtttgcctc
    47821 tgatttctca tccgtcagca gcgataaagc gccgtttatc tgggctgcac tgtcgctcta
    47881 ctgggcgcag atggccaatc tgatccccgg caaagcccgc gctgaatacg gcgagcaacg
    47941 tcaatattgc ccggtttgtg gttctatgcc ggtgtccagc atggtgcaaa ttggcaccac
    48001 tcagggtctg cgttacctgc actgcaacct gtgtgaaacc gaatggcacg tagtgcgcgt
    48061 aaaatgcagc aactgcgagc agagcggcaa actgcattac tggtcgctgg atgacgaaca
    48121 ggccgcgatt aaagccgaaa gctgcgatga ctgcggcact tacctgaaga ttctttatca
    48181 ggaaaaagaa ccgaaagttg aagccgtggc agatgacctc gcctctctgg tactggacgc
    48241 gcgaatggag caagaaggct atgcccgcag ttccatcaac ccgttcctgt ttccgggtga
    48301 aggggagtaa tctctgacgt tttccgggtg gcgctggtcg cccggattac agctcatagg
    48361 gtgtgacgac accatctctc gtattccgcg ttacccattc aagatcatgc cgcaattgtc
    48421 tgtttgcctc gttcttccct ccaacacatc gataaggtta tgggtatgtc actgatgaca
    48481 agcgtatgat tgcctgatgc gctgcgctta tcagtatcta ccaataaact tcaatatatt
    48541 gaatttgcat gactttgtag gccggataag gcgttcacgc cgcatccggc atgaacaacg
    48601 cgcacgttgt cagcaatctg attgacatca ttttttccca ttaccattcc tcgcgcagct
    48661 tacgctgata ttcgacacca tcttcctcac acccttgcca gatgccagga acactggttc
    48721 ttgctgtttg cgattgattt atcaggtatt gatctaccgc ttcccttaat aggtccgcac
    48781 gaggaagatt acgctgcacc tcgagatcat caagttgctt aatcacctca ttcgataaat
    48841 cgagtaaaat tctgcccata tctatacctg ccagagagtt cataaatatc tcgccaatat
    48901 catttttgaa tctatggaga gaaaagtacc cttgccgaat ctttaaagaa agctcattta
    48961 cgcatcattt tttatttttg cgaggcgttt tccagatacg atgtaacagt tgcagttatt
    49021 ataagttcaa gtaaaacagc cttgtattaa taccagaata agggcatctg tttttttata
    49081 ttcaagaatg aaaaattttt ttcattcctt atgctcctta cactgatcat caacaccaac
    49141 tacctccccc ttttgcctgc ctccctttgc tttacgcacc ggaaattgct gccgctccag
    49201 atattgctca accgcttcgc gtaaaattgc agatcgtgaa accttacgcc agccccgaag
    49261 tttgttcagc cgtttaattg cctcttctga taaatgaaga aaaaccgtat tcatcgccat
    49321 taccaccacc ttacctatat tactcattaa tgtatgtgcg aaaattacgc cggaaaatca
    49381 tttaaaaaaa gcggattttt gcatgttgtt ttgtttttgc gaggcgtttt ccagaaataa
    49441 taagagtgtt gcagttgttt catttattac tcaaaaatgg tttagttatc gcccaggatg
    49501 agttatggat gctatgaata tattatattc agggatgttt attttttgat tggatcttta
    49561 tttgcataat ctgaatggtg tacccgaaaa atgtcagcat cctaacagca cccggattta
    49621 aggtgaaatt aatcatccat cacagaccgg tcagtactgc caccaaccgg tctttacttg
    49681 ttcatcgtta taattttgtt ggtaacagtt cgggaaacaa ggaattcctt ccggaacccc
    49741 ccggtatcat ccgatagcat tgacgtcaat catcagcgcg acgatgagtt tttttaatcg
    49801 catcataggg tggcgacgtt cagcacgatt acgggtcata gcaaaactcc tgggcaggcg
    49861 accactatgc cgctttcaca ggagttctga atgctagtgc tttctgtatg gcataaccat
    49921 gcctctaacg gttaagtgct tgcagcacat gttgcggatc attggcaatc gctcgcagca
    49981 aagcctttgc aggcccggtc ggtgaacgtc gcccctgctc ccagttacgc aacgtgtcta
    50041 cgcttacaga aataagatcg gcaaatttag attgtgacaa ccctgaagcg cggcgtattt
    50101 ctttgatttt catagcatca acttcaaaag tccgcgaagg ctgtcgcgtt ccttcaataa
    50161 tttcattcat ctgggccatg ctgtgagtca accgctcaaa aagtgcctta tccatttacc
    50221 acctcgtatt taacaaccgg agcattgctt tttcctgggg agacagatca tctttaatac
    50281 ccttacgata aataagcagt aatctgatct cataatgttt aacctgatga aagtaaataa
    50341 ccctgacacc agcacgtttc ccctttcctc ctgacaccca tcgaaccttt cttaacccac
    50401 cagtttcagg tattacctca ccatagtcag gattcaaggc caggaaaaac tgaaaacgac
    50461 tgaattcatc gtcggtcagc agtttttgaa catcttcagt aaaaatttcc gtttcgatga
    50521 agagcataat tatacgccat tggcttagag taacaatcct ttttgtacgt cattgacgta
    50581 cccttgaaac cactagctta ctgatagtgc tccagcaaaa ccacgtctct gccgggtttg
    50641 tctttagatt tgcgaggcgc tttccagatt tttcacgccc gtgaatttcc ctgtatccat
    50701 ccagcaaaaa cgggtgtata agactgtaca ttcatacagc aaaataagtt ctactatggc
    50761 actggaaaaa ggaattgcag agttagtcga aaagtttatc gctgcgggtc gcccctcatc
    50821 gcgtcaacaa agtattgcgc agcgcaggga gggctatatc gccagcgctg tccttgcggg
    50881 tgagacagaa acgcgggtag atattcagac tatcgaactt gaagggatga ccttacgaat
    50941 agtgtcgccg ctgaatgccc cgacattact cccgacgata atttattact acggtggctg
    51001 ttttgttagc ggcgggttcg caactcacga caaccagctg cgccagttag cctattacgg
    51061 gcaatgtcgg gttattgccg tccagtatcg gctggcaccg gaacatactt tccccgctgc
    51121 ccatgatgat gcgcaacgag gtgcagaaat agtctggcaa cacgccgaac gactcggcgt
    51181 tgataaacaa cgtataactc ttgccggtga cagtgcgggc ggacatctgg cgctggtaac
    51241 tgctttgcga ttaaagcgtg ctggcgagtg gcaaccggca caactcatct taatttatcc
    51301 tatgcttgat gcgactgcgc atttcgaaag ctacatacgc aatggtcacg attacatcat
    51361 cacacgcgac actttactga gcggctttga gatgtatctg ccaggcattg aacgcaggca
    51421 tcctgaagcc agccctcttt ggcgcgatga tttcaatggc ttgccacctg tacatatcat
    51481 taccgctgaa tacgacccac tttgcgatga aggtgaagcg ttgtatcacc gcatgacagg
    51541 gcaaggagtg caatgcacct gccaacgcta ccttggcgtt atccacggat tttttcagtt
    51601 aggtggtatc agtgaggccg cgcgatcggc actacgggat gttgcctgga gagcgggaag
    51661 gtaacttcgg acctgggccc tgaaaaggtt ttcagattga aaaccttttc agtcaagtaa
    51721 aacgccccta ctcttcttcg ttcccgccct cttcatacgg gccaaatggc ttcgcgggca
    51781 gaacgatata cgtgccttca aacactgcac ccggcgtctc gtcgccaaag atttcaactt
    51841 gcatctgcac ccgtgctttt cgtccgcgcg ccagacggtc gagatcgccg cttaaggcac
    51901 caaggtcggc taccgcatga ggtttaccgc taatcggctt gctgtagcgg atatgcgcat
    51961 ccgccagaat aatcgttccg ccgagatggc gttcgcgcag catcagccag ataagccccc
    52021 aaccggtgag cgttgccagt gagaataaac tcccggcaaa cagcgtatgg tgcggattct
    52081 gattaccggt ttccggcatg gtggtgataa atttttgccc ggtatattgt tgaatacgca
    52141 cgcccatttt ttcactaagc gggatatgtt cgtaccacgc ctgttgcagc tgcgcgcacc
    52201 agtcgccgcg atgcaaaata tcatccagag tggcgacggg cttaatcatc aaaaaatggc
    52261 gaatcggcgt ggtggttggc gtggtgattt ctccctgatt aacaaacccc agcttggcga
    52321 aaaactccac cgcgtcttca cgggcgctac aggtcacgcg cttaacgcct tcctgacgcg
    52381 ccaccgactc cagggtcatc gccatcagcg ttcctaaccc tttgtcctgc acgtccgggt
    52441 gaacggccat aaagcgaatg gacgcttcat tgtcggcatt aatatacaat cggcctaccg
    52501 ccaccagatt accctgctcg tcgacgacca tctggtgatg cgccatcgca tcccacgcgt
    52561 cgcgttccga accttttggt tgatgcaggg gcttacgcaa catttcccag cgaaactgat
    52621 agtaacgctc taactcttct tctgtttgtg gaacccgaag gtgatacata gcgatactct
    52681 ctcttgtttc ccgtgacaac cctggaagct ggctcatacc tgcaaccaga atgtcacggg
    52741 gccatcattg accagcgata cctgcatatc ggcagcgaag cgtcctgttt gcgtgttcat
    52801 ctcttgctgg cggcagcgtt cgacgaaata gtcatataac gcctctgcgc gatccggtga
    52861 tgcgccttta gagaaacttg ggcgcatccc ccgttcggta tctgcggcga gggtaaactg
    52921 ggaaaccacc agtacactgc cgcccgcctg ttgcacgttg agattcatct tgccttcggc
    52981 atcgctaaag atgcggtagc cgagcacacg ctcgcacaga cggtttgctt tctgttcgtc
    53041 gtcatccttt tcgacaccca ataacaccaa aagtcccgcg ccaatttcgc ccgtcacttc
    53101 tccctccacg gtgacgctgg cacgggttac gcgttgaatt aatgcaatca tggttcgtcg
    53161 tcttcttctt gttcagctgc ttgttttagt ttgcggtatt ccccgagagt gacagtaatt
    53221 tccgcgccaa gcaagacgat acaccatgtc cagtagaccc aaacaaagag aatggggatc
    53281 accgccaaca cgccgtaaat gagctgatat gacgggaaca tggtgatata aagcgcgaaa
    53341 cctttctttc ctgcttcgaa caggagcgcg gcgacaaacg cgccgacaat cgcgtcgcgg
    53401 ttaggtacgc ggatggtagg aacaatgctg tacagcaacc agaaggagat ccacgacaac
    53461 agcagcggaa aaatacgcag cacgttatcg atgacagtat tgagatcgct cgcccagcgc
    53521 agggagagca aataggaact gatcgccaga ctggcccctg ccagcagcgg ccccagcgtt
    53581 aaaatcatcc agtacacggc gaacgagtaa atttttggtc gcgctcgttt actgcgccag
    53641 atggtattca acgcgctatc gatggagtac atcagcaata acgcggtgac gatcagcccg
    53701 cacgccccaa cggcggtcat cttgttggaa ttggcaacaa attgttcgat ataccgctga
    53761 ataacatcgc cagtagcagg cagaaagttg gcaaaaataa agtgacgtaa ctgaatgctg
    53821 acgtcggaaa acatgggaaa agcggcgaaa agcgcaaaaa caacggcaac cagcggcact
    53881 aatgagagca acgacacata ggcaaggtta cctgccaggg ttgtcatgtt gtcctcatca
    53941 atacgttgcc agagtagttt tagccaggcc catagtggac gggtacggtg cctggctttg
    54001 tcctgaatgg tttttagcat aacaccttcg cgaaatagtc cgggatggtg gttttatctt
    54061 tcaccagaat actggtaatg cccagctgat tggctccttc tatattatcg gcgttatcgt
    54121 cgaaaaagac tgtatcgctg ggtgaaaaac cttccgcctg caaaacatgc tggtaaattc
    54181 gtgcttcagg tttgcgcatc cccagatctt gcgacagata gatatggtca gcagcatcac
    54241 gaatttccgg gtattcttcc ggccagaagg tggtatgcag gcggttagta ttggaaagca
    54301 ccaccacgcg atgcccctgc tcacgcagtt tatgcatgat ggcgatcact tccgggcgca
    54361 gcgcaacaaa caccgcctgc cagccgtgag agaactgctc gtagcttagc ggcagggcca
    54421 tctcatgaca cagcgcctct gcgaacgctt cgtcgctaat ttccccacgc tcatgctgat
    54481 gaaacgcctc ccccatgtgg aaactcttct taagcgtagc cagtggaaca cgcgttaaat
    54541 cgctccaggc tcccagcaca cggttaaagt cgatatcgac aatcacatta cctaaatcaa
    54601 agatatagag catttttgcc tcctttcgcg ccacaagaaa ttaactgtag cgggaaagga
    54661 tgggtttggc tatgtggcaa ggtatgaagc caggttaacg gtcacaaaaa tctacgcgtc
    54721 ttcctgggga agaatgattt caatttggct gtcttgtaag cgtgatttta gcgcagcggg
    54781 cggcagctcg tcggtaaaga gcgcagtgac ctgtgccacg ttaccaattt caaccgccgc
    54841 cgaagcatga tacttagtgt gatcggcgac cagcagaata tttctcgcgt gcgccatcat
    54901 cgttttcacc acgttagctt cgtttacatc aaactccatc aacgcgccat cgctctcaat
    54961 cgccccaacg cttgttacca gataatcagc acgaaaatca gccacaaagg acgccgctga
    55021 agggccaatg atcccgctat tatgagagcg caacgtaccg ccgggcacca tcacttcaaa
    55081 gcgcgggttg tggtaaagaa tatgcgccac acgcaggctg ttggtgatta tccgcaaatg
    55141 attatggtta agtaacgccc gggcaacatg ctcaacagtc gtaccaatgg tgataaatat
    55201 tgttgaacca tcaggaatat agtctgccac cgcttcggca atcgcttttt tttcctcggt
    55261 ttgcgaaacc tcacgctgct cgaacgccgt attaacgacg ctggaagccc gacccgcgcc
    55321 accgtgatgg cgcgtaatca ggccttgctc gcttaattta cgaatatccc ggcggaccgt
    55381 ctgcgtggag acatccagca gatttgccag ctcatcaata ttcatatacc cacgctcggc
    55441 gatcagcatg aggagttggt cgtgccgcgg gttaccggtt agttcggtaa ggctcatgaa
    55501 aattcctcga aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa aagatcgggt
    55561 ttgatcacag tcagggatcc cggctcgtcc accgggacgt gtgcatttta acgccgctac
    55621 accgctggcg aagcggactg actccgctaa atccccactt gttgccagcg ccaccgccaa
    55681 agcgccgtga aaaacatcac ccgcacctgt ggtatctacc acatcaactt tgaaggccgg
    55741 ttgatgctga cgcccaccat tttccagcca gtcgcagcct gcgctaccct gggtcacata
    55801 gacatgacca tttgtgagcg tttgtgcctg ttttagcgca ctggccatct ctttcacgcc
    55861 cgttaagcgc gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg ccatcagctc
    55921 actgatatcc tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg cctgacgggc
    55981 cagggtgaag gcttttttag cgccgtcgtg ccagcgtaca tctgccagca caacatccca
    56041 ctgagagaaa tcaatttcct ccaaccactc tgcgtcaggc agcagatccg ggctggggta
    56101 gttaatgatt atccgctcgc ctttggtatc caccatgatg gcggattgcg aagatttcgc
    56161 ctggttatac cgtttggtgt aacgggtgtt aacgccccag gattccagct ctgccagcag
    56221 gctgttgccc gtgtcgtcat cacctacgcg accaataaaa tcgacctgcg cccccagcct
    56281 tgccgccgca accgctgccg tcgccgctgg cccgccacca acttccgtat aatttctcgc
    56341 cacgtattta ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat ccatcacggt
    56401 tatacctaca caagcaacac gaatcatggt cattccttaa acattttggg atgcgttcat
    56461 tttaatttca ccaattgttt aaaaagtgac ggctgtcaat tttttgacga taaattacaa
    56521 atacgatcaa aaacagacaa gcaaagcatg tcataaatga caaaaagtga catcactgta
    56581 ttcaggagag ggttatggca gcaatcgcgt ttatcggttt aggacaaatg ggttcgccaa
    56641 tggcgagcaa tttattgcag caagggcatc aacttcgcgt ctttgatgtg aacgccgaag
    56701 ctgtgcggca tctggtagac aaaggtgcga ctcccgccgc caacccggcg caggccgcta
    56761 aagatgccga atttatcatt accatgttgc cgaatggcga tctggtgcgc aacgtgttgt
    56821 tcggtgaaaa cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt gatatgtcca
    56881 ccatccatcc gctgcaaacc gataaattga ttgccgatat gcaagccaaa ggcttcagca
    56941 tgatggatgt tccggtaggc cgtacttctg caaatgccat taccggtact ctgttactgc
    57001 tggctggcgg caccgctgaa caagttgaac gtgccacacc gatcctgatg gcgatgggca
    57061 gtgagttgat caacgctggc ggtccgggca tggggatccg cgttaagctc atcaacaact
    57121 acatgagcat cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc gaagccctga
    57181 atcttccctt cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt aaaggtcact
    57241 tcaccacttc ctggccaaac aaagtcctca gcggagatct ttctcccgcc ttcatgatcg
    57301 atcttgccca taaggatctt ggcatcgccc ttgatgtcgc caaccagctg catgtgccaa
    57361 tgccgctggg ggccgcctca cgggaggttt atagccaggc gcgcgcagcg ggtcgcggtc
    57421 gccaggactg gtccgccatt ctggaacagg tccgtgtcag tgccgggatg actgccaaag
    57481 taaaaatgta acgactggat aaaggaataa atgaatgaat aagtacacca tcaacgacat
    57541 tacgcgcgca tcgggcggtt ttgccatgct ggcggtcgat cagcgcgaag ccatgcgcat
    57601 gatgtttgcc gcggctgggg cacccgctcc ggtagccgat agcgttttaa ctgatttcaa
    57661 agttaacgct gcaaaggccc tctcgcctta tgcctcggcg attctggtag atcaacaatt
    57721 ctgctaccgc caggtggttg agcaaaacgc gattgccaaa agttgcgcca tgattgtcgc
    57781 cgccgatgag ttcattcctg gcaacggtat tccggtcgat agcgtggtta ttgaccgcaa
    57841 aatcaatccg ctacagatca aacaagacgg tggcaaagcc ttaaaactgc tggtgctgtg
    57901 gcgtagtgat gaagatgcgc agcaacgtct ggatatggtg aaagagttca acgaactgtg
    57961 ccactcacac ggtctggtaa gcatcattga gccagtcgtc cgcccaccgc gtcgtggcga
    58021 taaattcgat cgcgaacaag cgatcatcga tgccgccaaa gagctgggcg acagtggcgc
    58081 tgacctctac aaagttgaaa tgccgcttta tggcaaaggt ccgcaacaag agcttctctg
    58141 tgcttcacaa cgtctgaatg accatatcaa tatgccatgg gtgatcctct cttccggtgt
    58201 cgacgaaaaa ctgttcccgc gtgccgtacg cgtggcaatg acggcgggcg catcgggatt
    58261 cctggcaggc cgtgcagtct gggcatcggt cgtcggttta ccagacaacg agctgatgct
    58321 gcgtgacgtt tgcgcaccga aattacaaca acttggcgat atcgtcgacg aaatgatggc
    58381 taaacgccgc taagatgagg ataaaaggaa tgaaatggtt taacacccta agccacaacc
    58441 gttggctgga acaggaaacc gaccgcatct ttgattttgg taaaaattca gtagtgccga
    58501 ctggttttgg ctggttaggc aataaagggc aaatcaaaga agagatgggc acccatctgt
    58561 ggatcaccgc tcgtatgtta cacgtttatt ccgttgctgc ggcgatgggt cgacctggcg
    58621 cttactcgtt ggttgatcac ggtatcaaag ccatgaacgg cgcactgcgt gataaaaaat
    58681 atggcggctg gtatgcctgc gtgaatgacg agggcgtggt ggatgcctcc aaacagggct
    58741 atcaacactt ctttgctctg ctgggtgccg ccagcgccgt cacaacgggt cacccggaag
    58801 cgcgcaagct gctcgattac accattgaaa ttatcgagaa atatttctgg agcgaagaag
    58861 agcagatgtg cctggaatcc tgggacgaag ccttcagcaa aaccgaagag taccgcggcg
    58921 gcaatgccaa tatgcacgcg gtggaagctt tcttgattgt ttatgacgtc actcacgaca
    58981 aaaaatggct ggatcgcgcg attcgcgtgg cttccgtgat tatccacgac gtcgccagaa
    59041 ataatcatta tcgcgttaac gaacacttcg atacccagtg gaatccgctg ccggattaca
    59101 acaaagataa cccggcgcat cgcttccgcg cgttcggagg tacaccaggc cactggatcg
    59161 aatggggccg tttaatgctg cacatccatg ctgccctgga agcccgttgc gaacaaccac
    59221 cagcatggct gctagaagat gccaaaggtc tgtttaacgc caccgtgcgc gatgcctggg
    59281 cacccgatgg cgcggacggg attgtttata ccgttgactg ggaaggaaaa ccggtggtcc
    59341 gcgaacgtgt acgttggcct atcgtcgaag caatgggtac ggcctacgcg ctctacaccg
    59401 tcactggcga tcgccagtat gaaacctggt atcaaacatg gtgggagtac tgcattaagt
    59461 acctgatgga ctatgaaaat ggttcctggt ggcaggagct ggatgcggac aataaggtca
    59521 ccaccaaagt ctgggacggc aaacaggata tttatcacct gctgcattgc ctggtgatcc
    59581 cgcgtatccc gttagcgccc ggcatggctc cagcggttgc agcgggtctg ctggatatta
    59641 atgcgaaata actgatctga aaatagggtg cgggttttcc cctcacccta accctctccc
    59701 caaaggggcg aggggaccgt ccggtgcagt ttttgggttt gacatcagcc tcacgggagg
    59761 ctgtatgtcg ttaattaagg tgccttgtat gcaaatcacc aatatgcatt gcagtggaca
    59821 gaccgtttca cttgccgcgg gggattatca cgcgaccatt gtgaccgtcg gtgctggtct
    59881 ggctgaatta acctttcagg gttgtcattt agtcatcccg cacaaacccg aagagatgcc
    59941 gctggcgcat ctgggtaaag tgcttattcc gtggccgaac cggatcgcca acggctgtta
    60001 ccgctatcag gggcaggaat atcagttacc cattaatgaa catagttcaa aagccgctat
    60061 tcacgggctg cttgcctggc gagactggca aattagtgaa ttaaccgcca ccagcgttac
    60121 cctgacagct tttctgccgc ccagttacgg ctatccgttt atgctggctt cacaagttgt
    60181 ttattcgctc aacgcacaca cgggtctttc cgttgagatc gcaagccaga atatcggcac
    60241 tgtcgcagcg ccttatggcg tgggtattca tacttattta acctgcaact taacatctgt
    60301 tgatgaatat ctgttccagc ttccggcaaa cctggtttat gccgttgatg aacacgtaaa
    60361 tccgacaacg cttcaccacg ttgatgagct ggatttaaat ttcacgcagg cgaaaacaat
    60421 agccgcgaca aaaatcgacc acacctttaa aaccgctaat gatctgtggg aaataaccat
    60481 tacccatcca caacaggcgc tttcggttag cctttgttgc gatcaaccct gggtacaaat
    60541 ttatagcggt gaaaaattac agcgtcaggg actggcagtt gaacctatga gttgcccgcc
    60601 taacgccttt aattcaggta tcgacttatt attactggaa ccaggaaaac cgcaccgcct
    60661 gttttttaac atttacggac agagaaaata acttgtattg attagcagtc aaaatcattg
    60721 aagaacattt cactcgctaa aggcaacatt atgtatcttc aatttattaa tttaaaaaca
    60781 gctcagcttc tgtaaagttt ttatatatca gttttatgag aatcatttta cataagcctg
    60841 taataacagg aaggcaacaa tctcgttctc aactctatgg agattagtta tggatacgcc
    60901 acgtccacag ttattagatt ttcaatttca tcagaataac gacagtttta ccctacattt
    60961 tcaacaacgt cttattttaa cccatagcaa agataatcct tgtttatgga ttggctcagg
    61021 tatagcggat atcgatatgt tccgcggtaa tttcagcatt aaagataaac tacaggagaa
    61081 aattgcgctt accgacgcca tcgtcagcca gtcaccggat ggttggttaa ttcatttcag
    61141 ccgtggttct gacattagcg ccacgctgaa tatctctgcc gacgatcagg gccgtttatt
    61201 gctggaacta caaaacgaca accttaacca caaccgtatc tggctgcgcc ttgccgctca
    61261 accagaggac catatctacg gctgcggcga acagttttcc tacttcgatc tgcgtggcaa
    61321 accgttcccg ctatggacca gtgaacaagg cgttggtcgc aacaaacaaa cctatgtcac
    61381 ctggcaggcc gactgcaaag aaaatgcgga cggcgactat tactggactt tcttcccaca
    61441 gcctacgttt gtcagcacgc agaagtatta ctgccatgtt gataacagtt gctatatgaa
    61501 cttcgacttt agtgccccgg aataccatga actggcgctg tgggaagaca aagcaacgct
    61561 gcgttttgaa tgtgctgaca catacatttc cctgctggaa aaattaaccg ccctgctggg
    61621 acgccagcca gaactgcccg actggattta tgacggagta acgctcggca ttcagggcgg
    61681 gacggaagtg tgccagaaga aactggacac catgcgtaac gcgggcgtga aggtcaacgg
    61741 catctgggcg caggactggt ccggtattcg tatgacctct tttggcaaac gcgtgatgtg
    61801 gaactggaag tggaacagcg aaaactaccc gcaactggat tcacgcatta agcagtggaa
    61861 tcaggagggc gtgcagttcc tggcctatat caacccgtat gttgccagcg ataatgatct
    61921 ctgcgaagaa gcggcacaac acggctatct ggcaaaagat gcctctggcg gtgactatct
    61981 ggtggagttt ggcgagtttt acggcggcgt tgtcgatctc actaatccag aagcctacgc
    62041 ctggttcaag gaagtgatca aaaagaacat gattgaactc ggctgcggcg gctggatggc
    62101 tgacttcggc gagtatctgc ccaccgacac ttacctgcat aacggcatca gcgccgaaat
    62161 tatgcataac gcctggcccg cgctgtgggc gaaatgtaac tacgaagccc ttgaagaaac
    62221 gggcaagctc ggcgagatcc ttttctttat gcgcgccggt tctaccggta gccagaaata
    62281 ctccaccatg atgtgggcgg gcgaccagaa cgtcgactgg agtctcgacg atggcctggc
    62341 gtcggttgtc ccggcggcgc tgtcgctggc aatgaccgga catggcctgc accacagcga
    62401 cattggcggt tacaccaccc tgtttgagat gaagcgcagc aaagagctgc tgctgcgctg
    62461 gtgcgatttc agcgccttca cgccgatgat gcgcacccac gaaggtaacc gtcctggcga
    62521 caactggcag tttgacggcg acgcagaaac tatcgcccat tttgcccgta tgacctccgt
    62581 cttcaccacc ctgaaacctt acctgaaaga ggctgtcgcg ctgaatgcga agtccggcct
    62641 gccggttatg cgcccgctgt tcctgcatta cgaagacgat gcgcacactt acaccctgaa
    62701 atatcagtac ctgttaggtc gcgacattct ggtcgctccg gtgcatgaag aaggccgtag
    62761 cgactggacg ctctatctgc cggaggataa ctgggtccac gcctggacgg gtgaagcgtt
    62821 ccggggcggg gaagttaccg ttaatgcgcc catcggcaag ccgccggtct tttatcgcgc
    62881 cgatagcgaa tgggcggcac tgttcgcgtc gttaaaaagc atctaatcac atccgcccgt
    62941 ggggaaactc atgggcaaag ggagaacatc gatgagtcac atcacaacgg aagatccagc
    63001 aactttacgc ctgcccttta aagagaaact ctcttacggt attggcgacc tggcctctaa
    63061 catcctgctg gatatcggta cgctttatct tttgaagttt tataccgacg ttctggggct
    63121 gccaggcacc tatggcggca ttatcttttt gatttcaaaa ttctttactg cgtttaccga
    63181 tatgggtacc ggcattatgt tggattcccg acgcaagatc ggtccaaaag gtaagttccg
    63241 tccttttatt ctgtatgcgt cattcccggt caccttactg gcgatcgcca actttgtcgg
    63301 cacaccgttt gatgtcaccg gtaaaacggt gatggccact attctgttta tgctttacgg
    63361 actgtttttc agcatgatga actgctccta cggcgcaatg gttcccgcta tcaccaaaaa
    63421 ccccaacgaa cgcgcctcac tggcggcatg gcgtcagggc ggcgctacgc tgggcctgct
    63481 gctgtgcacg gtgggattcg tgccagtgat gaatcttatc gagggtaatc agcaacttgg
    63541 ctatatcttc gccgccacgc tgttttcact gttcggcctg ctgtttatgt ggatctgcta
    63601 ctcaggcgtg aaagagcgtt atgtcgaaac ccaaccaacc aatccggcgc aaaagcctgg
    63661 cctgttgcaa tctttccgcg caattgccgg taaccgccca ctgttcattc tgtgtattgc
    63721 caacctctgc accttagggg cgtttaacgt caagctcgcc attcaggtct attacaccca
    63781 gtacgtactt aacgatccca tcctgttgtc gtatatggga tttttcagca tgggctgtat
    63841 tttcatcggc gtgttcctga tgcctggcgc agtcagacgt tttggtaaga agaaggtcta
    63901 tatcggcggc ctgctgattt gggtgctggg cgatctgctc aactatttct tcggcggcgg
    63961 ctcggtcagc tttgtggcgt tctcctgcct ggcgttcttc ggctcagcgt ttgttaacag
    64021 cctgaactgg gcgctggttt ccgacaccgt cgagtacggc gagtggcgca ccggcgtgcg
    64081 ttcggaaggc acggtctaca ccggcttcac cttctttcgc aaagtgtctc aggcgctggc
    64141 tggtttcttc cccggctgga tgctgacgca aattggctat gtgccgaacg tcgcacaggc
    64201 tgaccacacc attgaagggt tacgccagtt gatcttcatc tacccaagcg cactggcggt
    64261 agtcaccatt gtggcgatgg gttgcttcta cagcctgaac gagaagatgt atgtccgcat
    64321 tgtggaagag atagaagccc gtaaacgcac ggcgtaataa taataaacga cgccctgcgg
    64381 ggcgttataa ggagtgatta tgtctgacca taatccactg acattaaaac tgaatctgcg
    64441 ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat ttaatgctct gcatcggtac
    64501 tctgtatctc ctcaaatttt ataccgatga attagggatg cctgcttact atggcgggat
    64561 tatctttctg gtggcgaagt tttttaccgc gtttaccgat atgctcaccg gatttttact
    64621 cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt ccctttattt tatatgcagc
    64681 agttccggcg gcgttaattg caacgctcca gtttatcgcc accacctttt gtttgccggt
    64741 taaaacgacg attgccaccg cgctgtttat gatgtttgga ctttcataca gtctgatgaa
    64801 ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac ccgaatgagc gcgcgcagct
    64861 cgcggcttac cgccagggtg gtgcaaccat agggctattg atttgtaccg tggcgtttat
    64921 tccgttgcag tcgctttttt ctgactcaac cgtcggttat gcctgtgcgg cacttatgtt
    64981 ctccattggc ggctttattt ttatgatgct gtgctacaga ggcgtcaaag agcattatgt
    65041 ggacacagcg ccaaccggac ataaagccag tattctcaaa tctttttgcg cgatatttcg
    65101 taatccacca ttgctggttt tatgcattgc taacctgtgt actctggcgg catttaatat
    65161 caaactggcg attcaggtct attacaccca gtatgtgctg aatgatatta atttattgtc
    65221 gtggatggga ttcttcagta tgggatgcat cctcgtcggc gtattactgg tgccagtaac
    65281 tgtaaaatgt tttggtaaaa aacaggttta tttagctggc atggtgctat gggcggtggg
    65341 tgatatactg aattatttct ggggaagtaa ctctttcact ttcgtcatgt tctcttgtgt
    65401 cgcctttttt ggcacggcct ttgtaaacag cctgaactgg gcactggtac cagataccgt
    65461 agattacggt gaatggaaaa ccggtattcg tgccgaaggt tctgtttata ccggttatac
    65521 cttctttcgt aaaatttctg ccgcacttgc tggcttcttg ccaggcatta tgctgacgca
    65581 aattggttat gttcccaaca tcgcgcaaag cgatgcgaca ttacagggtt tgcgtcagct
    65641 catttttatc tggccttgtg cactggcaat tattgctgca ttgacgatgg gattctttta
    65701 cacactcaat gaaaaacggt ttgcattgat tattgaggaa atcaaccaac gcaaaaataa
    65761 agagatggcg acagaagaaa aaacggcttc cgtaacgtta taaatgtcaa tagccgctat
    65821 ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa tggacttatt atgaaaaaga
    65881 ttaatgcaat aattttatta tcttctctga cctctgcctc ggtatttgct ggcacatacg
    65941 tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg cgaggttatg ctacgtgtgg
    66001 gttataactt cgatatgggc gcgggtatta tgttaaccaa tacctacacc tttcagcgag
    66061 aagatgaact aaaacatggc tataatgaaa ttgaaggttg gtatccttta ttcaaaccaa
    66121 ccgataaatt aaccatccag cccggtggct taattaatga taagagtatc ggttcgggtg
    66181 gtgcagtgta tctggacgtc aactataaat ttgtaccatg gtttaatctg acagtacgaa
    66241 atcgctataa ccataataac tatagttcaa cagatttgag cggggaactg gataataatg
    66301 acacctatga aattggcacc tactggaatt ttaaaatcac cgataaattt tcctatacat
    66361 ttgagccaca ttatttcata cgcgtaaatg actttaatag cagcaatgga aaagatcatc
    66421 actgggaaat cactaatacc ttccgttatc gcattaatga acactggctg ccttatttcg
    66481 aattacgttg gttagatcgt aatgtcgaac cgtaccaccg cgagcaaaac cagattcgta
    66541 tcgggacgaa gtatttcttc taatgtgaaa ctgccggatg cggcgtaaac gccttatccg
    66601 gcctacggtt gatacggttt tcgttggccg gattaagcac atccggccat taatcaagct
    66661 tcagccgcca gcatctcttt cttacgttgg ctaattcgct taaccagcat tacggaaacc
    66721 accgcaccac agaatgcgaa gcaggccata atgccaaaca cgattttgta gccaataatc
    66781 cccggattta aatccagaat gtagccatac agactgaagc agaacatcgc cggagcgtaa
    66841 ccaataaagc tacccaacgc catcgccgcg cctgttttat tttcagcaat ttttgcttcg
    66901 ccgataggtg caaaaaatac ggctcgctgt gtaaagacta tcgcgccaaa gcccagcgta
    66961 catgccatcc ctaaatagac cggcatactt tcgtgcggca gcataatcaa cagtacgagc
    67021 gcagcggtac tgatgataaa ggtgtagcat agatatttac tcggcgattt caggatctta
    67081 tctgaaatca tgccaccaat cggtccacca atcattttca gacagtattg gttaatgatg
    67141 ccgtaagccc ccaccagcgc aacgggcaat agatagatgt ttttcaggaa tggaatgaag
    67201 aatgtcaggc cacagtaaac cgcgtaaacg aagaagacgt taaaagcgat aagccagatg
    67261 gttttgtctc tcagcaccga ggtcatactg gtgttttgcg atgctccgtc ttcttttttc
    67321 acctcaacgg acggtgcctc ttctttgtca ttcaggacaa agaaaataat aatgcctacg
    67381 gcaatcacaa taagggaata gaaccagatg cctgctttaa aacctaataa gccactgcca
    67441 aaccaggtaa atactgccaa cgcagaaaat gccaccacgg tatcgacaat gccacgccct
    67501 gtttcgaaga agccaaacaa ccgaccttgt tgttcgctat tgcccaatcg acttaccgac
    67561 ttgagcaaga ccggccagtt catcatgtcg caagtaacgc caaatagcgc ccagacaaag
    67621 aggatgcccc aataccccgg cattgtcgtc aaataaacac ccagtaatcc tgtcgcaatg
    67681 agtgagaatg acatggtgta tctgcgcggt agtttatcgg caaaataaat agacagaaaa
    67741 aagcccactg tggtgacaaa tgagtttacc gacatagcat taccaatttg accattggtc
    67801 aaatggaaat attcctgcat cgggatataa aacgcatctt tcagcgacgg taatttataa
    67861 attgtaccgc cacacaatgt taatagacta aataacgccc atttcttttt cgtgagcata
    67921 acatccccta agcgattttt aaaatcactg ttttaaaggc aataagatac cgaccattca
    67981 tatggaatgg ttttattaac tgcgtatgag ttaaaactta ttagtggttc agaagatggt
    68041 ttgcctcttg tttgatttgt tgtagtacgt tacgcacatg atttatctga ttaattgcat
    68101 cttctttttc tttacgtaac cgcacatcca gtaaatgatt ttctggcaat ggtgtttcac
    68161 tcatctggcg ataagggatc cacggattat catcttcgtc ttcaaagcgg aacgccggcg
    68221 catagtaatc aacctcttct tccaggccat atgccgtcac ctgcggctca tcatcaccca
    68281 ggcatataag gtgcgttaat aacgctttga acggcatatc accctgacct gaaatacagg
    68341 ctttatgacc caggccaccc ggttctttaa cgatcaaggc atctttgata tggacctggg
    68401 taatatgcgg cgccatcgtt tttaaagcgt cgatgggatg ctcatttgca ttgatcatgt
    68461 tcgcaaaatc aaacagtaag gaaagagatt ccatctcact ttctttgacc agcgacacca
    68521 gctcatggct ttttaaatct tcatgctgtt cgatcgtaaa agtcaggccg ctgtcctgat
    68581 acgtttcccg tacataggca atgtcgttag cgatgatcga taatacgtcg cgcaaattac
    68641 cttcataacg tggataaaaa cgtacggacg atgccccagt tttcaacgca atggcgacgg
    68701 cttcgtcaat agatgcctta tctgaagcgc tggtttcaat atgaatatca aggttcagac
    68761 ggcgggcttt atccccaaag gcagagagtt ctttatcgtc catattacca agagaaaaac
    68821 gctcgccatc cagaacatgg attttgacgc cacgtagatt attttcactt gcgatatcaa
    68881 gtaaatcagc aggcaacact ctttccagcc gcatatttaa atgaaaggca taagcatgta
    68941 aataaagagg aagagtgtcg acacgttgta gaatctttct tgcgttattt attgtcacca
    69001 taaaacacct acagattaat gatgaggtct acggcctcaa atcgccaata ttcaatatct
    69061 atctggacaa tattgccttg ctggtcggcg cgatgctttt caattaagat cgctggcata
    69121 cccgcagaac cgccaagata cttgctaata tcgcccggca ttctgacggg tttgaaaatc
    69181 aattttttta ctaccgtctc tttttggtag cacttttccc agactgcaga aaatgagtgg
    69241 ttttcaagtt gttcaataaa acccggagca acttcaggat taatatatgt ttcgtgatag
    69301 aaaactttat gtccttccag cgccccccag ccggtgatgc gatagagttc agttgatggt
    69361 gtcactgcaa tcagtggcgc gagcgtcttg ggaatatcgc tggtacggtt tttctcggta
    69421 aacccccagg aaggctctcg tccttgctca attgcggccc gctgaaagct cgccgacaac
    69481 tccggactgt aattaaatcg cggctgggtc acgaaccacc ccttacgatc cttacggaag
    69541 attttggatt ctgcctcgag gttcaacaac gcctggcgca gcgtcatacg tttaatgccc
    69601 agcagttctc ccagctctct ttctgaaggg agttttccac ccggcgtggt gatgccatct
    69661 ttcaaccacc gatccagttt ctcttttgca ttttctacgg tggattgatt ctccgccatt
    69721 gtttaatcct tttggtttaa accagatgaa gcatcttacg cttacgccaa aaaagaaagc
    69781 aattaacggc gtatgacttt gcagataatt gtgacaaagc tctctattga cgtaatccca
    69841 gacttataag ggagatactc gcattggtgt gcgctcacga caggtaaaaa aaacctgcca
    69901 gcgatggcag gttttttata actaaaaaac gaaattaatc gtctttcggt gcgcggttgg
    69961 cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag gtcggagtca
    70021 cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc atgcggatag
    70081 gcggaaccag aacaacggct tcgtcagtac cggaagcacg catgttggtc agtttcttac
    70141 cggtcaggca gtttaccgtc aggtcgttag agcggctatg aataccgata atctgacctt
    70201 cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga ccgaacagcg
    70261 cgaacgcgac cgctttaccc tgaccgttag agatcagtac gccgttctga cgctgaccca
    70321 cttcacccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga ccagtaccgg
    70381 aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg atcacgtagt
    70441 cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct ttacgttcgc
    70501 ccagcgcctg cattacagaa ccctgatgct gttcttcaac gtccagagtc acgttttcat
    70561 acggctcttg tttacgaccg tcgatttcac ggaagataac tttcggacgg gataccgcca
    70621 gttcgaaacc ttcacgacgc atgttttcga tcagaacaga caggtgcagt tcgccacgac
    70681 cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca acgttgtgta
    70741 ccagttcttt gttcagacga tccaggatct gacgagacgt tacgaactta ccttctttac
    70801 cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca tcaacggaga
    70861 gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg ttcagttcgc
    70921 caaggcccgt gatcgcaacg atatcgccag cttccgccag atcggtttcg atacgttcca
    70981 gaccgaggtg gcccagcact ttaccgactt tcgcgttgcg ggttttgcct tcgctatcga
    71041 tgatagtgac ctgctggttc ggcttcactt taccgcgctt gatgcggcca atgccgataa
    71101 cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga ccgtcaaggt
    71161 caacgtccgg cgcaggaacg tggtcaacaa tcgcctggta cagcggggtc atgtcttccg
    71221 ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa acgatcggga
    71281 agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc tgatccacaa
    71341 cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc ggcttcaggc
    71401 cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg tcaaatgcgt
    71461 caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca ccaccgaagt
    71521 cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg atagcggtgt
    71581 ttttcgcgag gatggtaatc ccccgctctt tctccaaatc gttggagtcc atcacgcgct
    71641 cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg tctaccaggg
    71701 tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt tcgatcacaa
    71761 ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc actacaaaac
    71821 aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat cgctttcacg
    71881 gagcataaaa agggttatcc aaaggtcatt gcaccaacat ggtgcttaat gtttccattg
    71941 aagcactata ttggtgcaac attcacatcg tggtgcagcc cttttgcacg atggtgcgca
    72001 tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta tcttttttac
    72061 ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgaccatgac caatccagga
    72121 gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg aagtgaagtt
    72181 tgttgatttg cgcttcaccg atactaaagg taaagaacag cacgtcacta tccctgctca
    72241 tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct cgattggcgg
    72301 ctggaaaggc attaacgagt ccgacatggt gctgatgcca gacgcatcca ccgcagtgat
    72361 tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg aacctggcac
    72421 cctgcaaggc tatgaccgtg acccgcgctc cattgcgaag cgcgccgaag attacctgcg
    72481 ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct tcctgttcga
    72541 tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg atatcgaagg
    72601 cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc cggcagtgaa
    72661 aggcggttac ttcccggttc caccggtaga ctcggctcag gatattcgtt ctgaaatgtg
    72721 tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag tagcgactgc
    72781 tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg acgaaattca
    72841 gatctacaaa tatgttgtgc acaacgtagc gcaccgcttc ggtaaaaccg cgacctttat
    72901 gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt ctctgtctaa
    72961 aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc aggcgctgta
    73021 ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa acccgaccac
    73081 caactcttat aagcgtctgg tcccgggcta tgaagcaccg gtaatgctgg cttactctgc
    73141 gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag cacgtcgtat
    73201 cgaagtacgt ttcccggatc cggcagctaa cccgtacctg tgctttgctg ccctgctgat
    73261 ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg acaaaaacct
    73321 gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct ctctggaaga
    73381 agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg tgttcactga
    73441 cgaagcaatt gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg tgcgtatgac
    73501 tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt tgtactaccc
    73561 ggcgcaacaa cgccggaggt tcaaaccagg cccatctggc gtaattgttg cagccagttt
    73621 gaacacggac agcgcgcaga acccggagcg tacactggta cgtgaggagt tcgagcactg
    73681 cccaggttca aaatggcaaa taaaatagcc tgatgggact ggtttttagt tgccgtggaa
    73741 atttcagccc atctctgcat gggctttttt ctccgtcaat tctctgatgc ttcgcgcttt
    73801 ttatccgtaa aaagctataa tgcactaaaa tggtgcaacc tgttcaggag actgctttat
    73861 ggcaacaggc acgcagcccg atgctgggca gatcctcaac tcgctgatta acagtatttt
    73921 gttaatcgat gacaacctgg cgatccatta cgccaaccct gccgcgcaac aactgctcgc
    73981 ccaaagctcc cgcaaattgt ttggtacacc gttaccggaa ctgttgagct acttctcatt
    74041 aaatatcgag ctgatgcaag aaagtctgga ggcggggcaa ggttttaccg ataacgaagt
    74101 gacgctggtc atcgacgggc gctcgcatat cctttctgtg acggcccagc gtatgccgga
    74161 cggcatgatc ctgctggaga tggctccgat ggataaccag cgccgcttaa gtcaggaaca
    74221 gctacagcac gcccagcagg ttgctgcccg tgatttagtg cgcggcctgg cacatgagat
    74281 taaaaatccg cttggcggtt tacgtggcgc ggcgcagctg ctcagcaaag cgttacctga
    74341 cccatcacta ctcgaatata ccaaagtgat tatcgaacag gcggaccggc tgcgaaatct
    74401 ggtcgaccgt ctgttggggc cgcagctgcc cggtacgcgc gttaccgaaa gtattcacaa
    74461 agtggctgaa cgcgtggtaa cgctggtgtc gatggaactg ccggacaacg tgcggttgat
    74521 tcgtgattac gatcccagcc tgccggaact ggcgcacgac ccggatcaaa ttgaacaggt
    74581 cttgctgaat attgtgcgca atgcgctaca ggcgctgggg ccggaaggcg gtgaaatcat
    74641 tctgcgtacc cgcaccgcgt ttcaactgac cttacacggc gagcgctacc ggctggcggc
    74701 gcggattgat gtggaagata acgggccggg cattccgcct catttgcagg atacgctgtt
    74761 ttacccgatg gtcagcggcc gcgaaggtgg caccgggctt ggcttatcca tcgctcgtaa
    74821 tttgattgat cagcattcag gcaaaattga atttaccagt tggccagggc ataccgagtt
    74881 ctcggtttac ctgcctatca ggaaataaag gtgacgttta tgcaacgagg gatagtctgg
    74941 gtagtcgatg acgatagttc catccgttgg gtgcttgaac gtgcgctcgc tggggcaggt
    75001 ttaacctgta cgacgtttga gaacggcgca gaagtgctgg aggcgctggc gagcaaaacg
    75061 ccggatgtgc tgctttcaga tatccgtatg ccgggaatgg acgggctggc gctgctcaag
    75121 cagattaaac agcgccatcc aatgcttccg gtcatcatta tgaccgcaca ttccgatctg
    75181 gatgctgccg tcagcgccta tcaacaaggg gcgtttgatt atctgcccaa accgtttgat
    75241 atcgacgaag cagtggcgct ggttgagcgc gctatcagtc attaccagga acagcagcag
    75301 ccgcgtaatg ttcagcttaa cggcccaacg accgatatca tcggcgaagc gccagccatg
    75361 caggacgtgt tccgtattat cggtcggctt tcgcgttctt ctattagcgt gctgattaac
    75421 ggcgaatccg gcaccggtaa agaactggtc gctcatgccc tgcatcgcca cagtccgcgc
    75481 gccaaagcgc cgtttatcgc gctgaatatg gcagctatcc caaaagattt gatcgaatca
    75541 gaactgtttg gccacgagaa aggcgcgttt actggcgcga ataccattcg tcaggggcgt
    75601 tttgaacagg ccgatggcgg tacattattc ctcgacgaaa ttggtgatat gccgctggat
    75661 gtgcagacgc gtttgctgcg cgtgctggca gacggtcagt tttaccgcgt tggcggctat
    75721 gcgccggtga aagtggatgt gcggattatc gctgccactc accagaatct cgaacagcga
    75781 gtgcaggaag gtaagttccg tgaggatctg ttccaccgcc tgaacgttat ccgcgttcat
    75841 ctgccgccgc tgcgcgaacg tcgggaagat attccccgtc tggcgcgcca ttttttacag
    75901 gttgccgcgc gcgaactggg cgtagaagcg aagttactgc atccggaaac cgaagctgct
    75961 ctgacgcgtc tggcgtggcc aggcaacgtg cgccagctgg aaaacacctg ccgctggcta
    76021 acggtgatgg ccgccgggca ggaagtgttg attcaggatt tgcccggcga actgtttgaa
    76081 tcaacggttg cggagagtac ttcgcaaatg caaccggaca actgggcgac gcttcttgcg
    76141 cagtgggcag acagagcgct gcgttccggt catcaaaatc tgctttccga agcgcagcca
    76201 gagctggagc ggacgttact gacgaccgcg ttgcgacata cgcaggggca taaacaggaa
    76261 gcggcgcggc tactcggctg gggccgcaac accctgacgc gtaagttaaa agagctgggg
    76321 atggagtgat tcacagcttg tgtgtaaaga ttgattattg agcgcaaatt gctggtattt
    76381 tacgctttac tgttccgata agttcagtat gatcttgccc ggaaaacggg gagagtcatt
    76441 atgctggaat caataattaa tctggtatcg agtggcgcag ttgacagcca cacaccgcaa
    76501 actgccgttg ctgccgtgct gtgtgccgca atgattgggc tgtttagttg agtttcagat
    76561 tgctgatgaa gtgctctttg ttcatgccgg atgcggcgtg aacgccttat ccggcctaca
    76621 aaagcaatat tttcaacagg ttttgtaggc ctgataagcg tagcgcatca ggcaatcttg
    76681 ttctcagcac attgatggcg ctttcgtttt ctactttgta aacgaagcgc cattcactat
    76741 ttaaatcacc cgtgagaact gctgcatccg cgctttctgg cgcagatagg tatcaaagca
    76801 catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc acctgtattc ccttctcatc
    76861 cacatccacc agcccatctt ttgctaacgg ggcgagcagc ttgagatctt ccgcaaagta
    76921 atcagcgaag tgcaaatccc actgtttctc aataggggcg taatccagac ggaagttgca
    76981 gatgagcgac ttaatcacat cgcggcgaat acagtcatca cgcgttagcg caataccacg
    77041 ccacagcgca ttgccttgtt catccacttg ctgatagtac tgcttcaact ctttctggtt
    77101 ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg cccatcccca gcagatcggt
    77161 atcgccctga gtggtgtagc cctggaagtt acgatgcagc acgccttcac gctgggccac
    77221 cgccagctcg tcatccggac gggcaaagtg atccataccg ataaactgat agcccgattg
    77281 cgtcaggaag gcgatggttt cctgcaggat atcgagtttt tgctgcggac tcggcaggtc
    77341 agcatctttg attttgcgct gagcagcaaa aatggtcggc agatgcgcgt agttaaagac
    77401 actcagacga tcggggttca gctccgccac acgtttcagg gtaaaggcga aactctccgg
    77461 cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg gaggtaaaac caatctcacg
    77521 cgcatggtta agcagtgcaa agatgaactc ttcatcctgc tcgcggttaa ccagacgttg
    77581 cacttctttg ttgaagtcct gcacgcccat gctcaggcga ttaaagcctt cggcgcgtaa
    77641 atgatcgagt acatccagtt cgatttcccg cggatcgact tcgatcgaaa tctccgcatc
    77701 ggcattgaac tggaagtttt cgcgcagcag cttcatcagg cggctgattt gcgctttatt
    77761 cagatacgtc ggcgttccgc cgccccagtg caattggctg acgtgacgcc cggcgaacag
    77821 cggtgcacga tggacgattt cttgctccag cgcgtccaga tactgatcgg ccttgtgctg
    77881 ctggcgagta acaatcttat tgcaaccgca gaagtaacaa agcttatggc agaacgggat
    77941 atgtacgtag agagataatg gacgctcagg atagcgcgcc acggcttgta aaaacgcctg
    78001 ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac gaggtgtatc gtggcccgga
    78061 atagttatat ttctggatca gggccagatc ccagtcgatt tgctgtacag acatgctcac
    78121 tccttccaat ggcgtctctg gcggctacgg cgtaccggcg gatgcccgtt tcgggtcatc
    78181 agccggttgc gcagccactt ttgtcgccgc gacaaccgct gtagtttaac gaataaccac
    78241 accagataac atataaccgg aagggttatc agcagaacgg gaaggcccac ggcggcaatc
    78301 cgttagttgc cccgcagcag acgcatcatg tcttcttgct tctcgtcttc ttcctcttct
    78361 tcgtcatcgt cataagagag gccgagtttc tgcatcaact catcaatacg atccagttta
    78421 gcatccaccc aggattgctc ttcggcgctc agggtttcgc ctgcttccag acgttccagc
    78481 agcgcatcca gacgctcatc cgtttccagt aattccaact ccgcctgcgg tgaaagcata
    78541 ggtttctcac tcttcggttt gtgctgtttg gtgacttttt cagtcacgcc caatggaata
    78601 ggggttttac tgccaatacg tggatctttt ggtgcgttct ggcctttgct gcctgacgtg
    78661 gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac gacgtttttt ctgacgcttg
    78721 cgatcacgag cttcctgatc cagctcctcg cgtgtttttc gacgcgcttt tgcatgacct
    78781 ttgctgcgtg agtttgaaga tgatggtttc ataatggttc gtcttttagc cgttttattc
    78841 agtatagatt tgcggcggaa tctaacagaa agcaagcaaa gaaaaaaggc gacagattac
    78901 tctgtcgcct tttttcctga ctcataaccc ttaacgggtc ttacaatcct tgcaggccgc
    78961 caacacaccc tgtttttcct ttagccggaa caatccgtgt tgattccatt tccgtttaat
    79021 tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct tcgcctcctg gcgctcctga
    79081 ccctcatcct gagtcattgt tcctgttagc gtcctcgctc tatgggtgct actttacgcc
    79141 tttgctttaa acaaacaatc aaccgccatc agtatttgca cattttcaga gttttatata
    79201 aaagcaattc actgatttat ataacttatt tgacatgcat cgcagaattt tctctgctat
    79261 ttcgcataaa gactatcggc aatgtctcac aaagcgtatg gcttttactt acaacgctca
    79321 cgccgaaaaa tccgcctttt attatggttc aggtataatc gccacaaagc tttgactgac
    79381 ggagacgacc gctttgacta atttgaatta tcaacagacg cattttgtga tgagtgcgcc
    79441 tgatattcgc cacctacctt ccgataccgg aattgaagtg gcttttgcag gccgttccaa
    79501 cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag aaaagcctgg ctcgtacctc
    79561 aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa gtggctgacg gcaagcgtct
    79621 ggttgacttg cctgggtacg gttatgcgga agtcccggaa gagatgaagc gcaaatggca
    79681 gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg caaggtctgg tggtactaat
    79741 ggatattcgc catccgctga aagatttgga tcagcagatg attgagtggg cggtagacag
    79801 caatatcgcc gttctggtgc tgctgaccaa agcggacaaa ctggcaagcg gcgcacgtaa
    79861 agcgcaattg aatatggtgc gtgaagctgt actggcgttt aacggtgatg tgcaggttga
    79921 aacgttttct tcgttgaaga aacaaggcgt ggacaagctg cggcagaaac tggatacctg
    79981 gtttagcgag atgcagcctg tagaagaaac gcaggacggc gaataatttt cttgccttaa
    80041 tgcttatgcc ggatgtggcg tatccggccc gtaaattcaa tagattacaa ttctaccgtt
    80101 ggcctgataa acatagcgca tcaggcatta cggatctttt ctttcgccca ataaaaaacg
    80161 ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt acgtgaagta
    80221 aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact ttactctttt
    80281 ttgttcagtt cagaaagcac tttttgtaat tttttttcag ttgttgcata ggaatttcta
    80341 atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg gcatgttcag
    80401 gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc agcaacggca
    80461 catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg gcatcaacat
    80521 catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata cgtacacgcg
    80581 gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc ccgtttgata atgtcggcgg
    80641 cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga cgagcaccat
    80701 tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca acgtagccct
    80761 gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg tagcgttcga
    80821 agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc cgcgccagac
    80881 cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta cggcgttgct
    80941 cgctggtgac ggtttccagt ggcaaaccaa acacttctgc cgccgttgcc cggtggatat
    81001 cttttccttc cgcgaatgcg gtcagcaagc ctttgtcacg cgaaagatgc gccataatgc
    81061 gcagttcaat ctgcgagtag tccgctgaga caatcacata atcctctggc gcaataaacg
    81121 cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg ttaggatcgg
    81181 ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc acacgcccgg
    81241 ttttcgggtt gatcatcagc ggcagcttgt cggtgtaggt cgatttcagc ttcgccagac
    81301 cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct tccagtacct
    81361 cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaata ccctgttttt
    81421 caaagagaat ggtttgcaac tgcttggtgg aagaaaggtt aaattcttca cctgcaattt
    81481 catgcgcttt cttttccagc tcagccagac gcaaggtgag ctcttcagaa tgattgtgca
    81541 gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcacc ggcaccagcg
    81601 gcatttcgat attctcgaag acgttcaacg gccctttgtg tttttgcaga tctggccaca
    81661 ttttcagatg caactgcaag gtgacatcag catcttcggc ggcgtaacgt ccggcctctt
    81721 cgagggcaat ctggttaaag gtcagttgat ttttgccttt acccgcaatc tcttcaaaag
    81781 tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga cgcccggcaa
    81841 cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc agctcaatgc
    81901 cgtaattcgc cagaataccg cgatcgtatt tcaggttttg cccgaccttc agcgcctttt
    81961 catcttccag cagcggtttt agcaactcga gtgcacgctc gcgagagatt tgatcgggcg
    82021 cgtcaagata atcatgagca accggaatat atgccgctac gcctggctcg atagcaaaag
    82081 aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta tcaaatgcaa
    82141 ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca tcaaggatgg
    82201 tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg tctgcaacac
    82261 tggtttcctg cggcttcgcg gctggttttg cccctttggc ctgtaaccat ttgcccgctt
    82321 cgacatcagc agtccagcgt ttgaactcat actttttgaa tagccccaac aactcttccg
    82381 ctgccggttg ctgcacttcc agttgttcac aggtcagctc cagttcaacg tcggttttaa
    82441 tcgtcgccag ctggtatgag agataagcaa cttctttgtt ttgctcgagc ttcgctgcca
    82501 ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca tacagcgtat
    82561 ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt ttcaccgacg cccggtacgc
    82621 caggaatgtt atcggaggag tcacccatca gcgccaggaa atcgatgatc agttctggcg
    82681 gcacgccgta cttattcacc acctcttccg gtccgaggat ggtattcgtc atggtattga
    82741 taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgccagtg ctgatcagca
    82801 ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc gataacgtcg tccgcttcta
    82861 cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc aggggttcga
    82921 tttgtgctcg cagatcgttc ggcattggcg ggcgatgtga tttgtaatgt tcaaacagtt
    82981 catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc ggtttatatt
    83041 gcatgatcag actgcgcagc atgttgagga caccatacat cgcaccggtc ggctcgcctg
    83101 cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat gaaccatcta
    83161 caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt tatcagatta
    83221 tgggtaaagg atgccacaga caggatgaaa acatgagttt ttcaccgctt ttcgtgaaaa
    83281 tcttttgtag atcttctgga tcgctcgcaa atcgtcatgt ggataacttt gtgcataaat
    83341 tatttacatc gcttttaatt tattatcatg ctttttacag catgatattt gtctttgttt
    83401 ttcatgcagt taaaaataaa gccatcccta ataattgaga taatgggatt ttttctaatg
    83461 tggatagata tgaattattt ttctccttaa ggatcatccg ttatttgggt cgttttctgc
    83521 taaaatctgc gccttgcgct tctttagcgc actgtctatg gctaaccttt tgaataaatt
    83581 cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat tgaccatttt
    83641 ggtcactatt ttttgttccg tcccgatcat cattgccggg attgtaaaac tattgctacc
    83701 tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt attgctggtg
    83761 tgaaggtctg gcggtattac tgcatctgaa cccacacctg caatgggaag ttcacgggct
    83821 ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta gttgggcaga
    83881 tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat atttcctcaa
    83941 gcaacagctg gcctgggtgc catttcttgg cctggcgtgc tgggcgctgg atatgccgtt
    84001 tatgaagcgt tattcccgcg cttatttatt acgtcatccg gagcgacgtg gtaaagatgt
    84061 tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca ttgtcaattt
    84121 cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acccactcca cttttcaaaa
    84181 cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg gtaaacaatt
    84241 cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc cgttcttcga
    84301 tatgttaagc ggtaaactga cgcgaattgt cgttcatgtg gatttacagc ctattgccga
    84361 tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc agcaatggct
    84421 gaactcactg tggcaggaaa aagaccaact tctgacgtcg ttgatgtcat cacagcggca
    84481 ggacaagtaa attttgctcg cctctgaatg agcagaggcg agtgagtatt ttaatgaaca
    84541 aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt aaaaacaact
    84601 ttacccggtg tataacgcag ttgcagcgag gcggtattgt tgtcaatcgt gattaactga
    84661 aacatctgac ctaaggtcat catcccgctg atttgtttat cagcccgctt ttgcgctttt
    84721 tccgcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac gacattaagc
    84781 ggtaacttca catcaaaatt gagcgattta atatctttgt ttgtggatga tgaagatttg
    84841 gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc gttcttccat
    84901 tttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata ctctttgaac
    84961 aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg gtgtgcagca
    85021 agctgcttct gcattgcaat gttatactgg atgataaatt ggcgcaccgc tgagggatca
    85081 atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc ttttgttaac
    85141 ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac gcgatccagc
    85201 atggtttttg caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt gataatgtta
    85261 atctgattaa atttcgactc gctttcacca accggaaatt tcttttcttc taatcgcgcc
    85321 agagaatcaa tggtaaaact ttttgccgtt actttgttca ttggtgataa ttgcagaaca
    85381 atatcttcga ctttgccttc aatatccagg ttagacaatg aactaccatc accattaaaa
    85441 gtaaactggc cctcaccaaa agaaaacttt ccatactctg caggggcaac atctaatgtc
    85501 gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga tttatttttc
    85561 gcggcgataa atagcggttg tgtcagttca ttattcacta agttcacttt tgccgctgcc
    85621 agtgctggga tgacattacc atgcattaac attgtgatgg gcaacggacc gtgctcaaca
    85681 tccacatcaa atacaacttt ctggcctggc ttgatattga gatcgggtgc accgttatcg
    85741 aatgtcattt gcatttgaaa acgggaatta aaaaagcctt tgtcaaagtt tttataactt
    85801 aacgtcatat cgtaggcatg ttcacctttc tttttcgcat cgttaaaatc tttaataaac
    85861 ttttcgatac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc cagattacgg
    85921 ctaacgcaac aataacacct gtagctgact tacgtatcat ataaatattc cctgataaaa
    85981 cagcaaaact ttgaatatcc acttatgctg aaataatgtc cagcggcagg atgcattatc
    86041 agaatgtatt tatatttatt aaataataaa aaaagcccgt gaatattcac gggccttatg
    86101 taatttacat tgaattattt tttctcggac agatatttca ctgtatcagc atactgctga
    86161 acaaaaacat ccatattgct ggtatccata ccctgcggat tcagctgata tttaccgtta
    86221 acaaacatcg ccggaacgcc acgcaattgc acgtcagctg cagctttttc ctgctgagcg
    86281 accagagatt tgaccacgaa gctgttccac gccgcgtcgt actcttcacc tttaatacct
    86341 gcgttgataa atacatcgcg gatatcagat gctgaacgaa tggtctgggt tttctgtacg
    86401 ccttcaaaca gcggaacagt gactttgtct tccacgccca gcgccatcgc cacagcccat
    86461 gcctgagtca gatctttgcc caggtcacca cccatgaagt tgacgtggta tttagtcatc
    86521 ttcacgcctt ccggcagttt tttcttcaca ttatcagaaa tatgcagaac ttcttcaaac
    86581 tgatagcagt gcggacagaa gaaagagaaa aactccagca cttgcggcgc gccagctacc
    86641 ggtttttcca gggtagtgta ctgtttacca tcttcatact gcgccgccga tgcgctaaac
    86701 gctaaaacta aaccagccag cgccagccaa atctttttca tgatctactc tctccgatta
    86761 atacataggt gttaattgta aagggggttc ttgtagaact tttgcctgtt ctataaaagt
    86821 cgccgtttgt cgcagccagt aatcttcccc ggttaaccac gggaaatttt tcgggaacgc
    86881 gggatcagcc caacgccgca ttagccaggc aagataataa accaaacgca tggcgcgtaa
    86941 aggttcaatc agtccgattt cagcggtgtc gaactcgcta aattcttcat aagcttcaat
    87001 aatagtttcc agttgcatcc gctgctcggc tttatcgcca ttgagcaaca tccacaaatc
    87061 ctgaatagct ggaccattac gtgcatcatc cagatcaaca aacattggac catcgcgcca
    87121 gagaatattc ccggcgtggc agtctccatg tagccgcaga acggtgaaat cttcccgcca
    87181 gtgcgctgta acggcggcga tcagctcatc tgtcgctttc aggaatgccg ctttcaaccc
    87241 ggaaggtatc agtgtagcgt cctcaaacag cttgcgtggc tcaatgagat attcattcag
    87301 accgatggtc gggcgatgga taaaaagctg tttgcgcccc gtctggtgca tacgccctaa
    87361 ataacgccca accgcttcca tctgatcgat attatcagct tcgaactggc gaccaccgac
    87421 gcttggaaaa acagcgaaat aaaatccctg atgattcaat aaagtctgac cgttaaaggc
    87481 cacaggtgct gcgaccggaa cttcatcatt taccaactgc aacgcaaatt gatgttcttc
    87541 gaggatttga tcggctgtcc aacgttcagg gcgataaaat ttgacgacaa aacgtcgacg
    87601 atcttcgtcc tgaaattgat agacacggtt ttcatagctg ttaagcgggg taagaccgga
    87661 atccacccgg atcccttgct caaatagagc atccatgatg gtatccgggt gtagtgtctg
    87721 gaaagtaaaa gcgctgttgt tcatcccatc atccggaaaa cattacgaat gatgaaggat
    87781 atcatttcac gatgattttg gtggcaccgc ttacaagctt ttactcttta attacgccgc
    87841 gagcgcgtaa cagcgccgtt ttgaagtctt cttcataatc tttctgcaaa cccgggatca
    87901 ccgcatcttt cgcggaatca cgcattttca ggtgatagat cagaatgtca tccgtcaaat
    87961 ccgccagttc gccgtcaaaa cctgactctt tcgccagttt ctgcaaaaat tgcaacaggt
    88021 taaggtctgg ctctttttgc caggctggct ggaggagttc aataacttca ttcagacgtt
    88081 tacatttcat agtaatgctc cttactcttg agacagacac gttagcaggg tcaatcccac
    88141 aataaaagag gcgatatcgg tgaatctgat gacgacgata acaggcgttg tgctggcagg
    88201 cggtaaagcc agacgaatgg gcggcgtaga taaaggattg cttgaattaa acggcaaacc
    88261 attatggcaa catgtcgctg acgcgcttat gacgcagctc tctcacgtcg tggttaatgc
    88321 caatcgccat caggaaatct atcaggcaag cggtctgaaa gtgattgaag attcactggc
    88381 ggattaccca ggccctctgg caggaatgct ttcagtaatg cagcaggaag caggcgagtg
    88441 gtttttgttt tgtccgtgcg atactcctta tattcccgag gatttagcag cccggcttaa
    88501 tcatcagcgc aaagatgcgc ctgtcgtgtg ggtccacgac ggtgaacgcg atcacccgac
    88561 tattgctctg gtaaaccgcg caattgagcc tttattactg gaatatctgc aagcaggaga
    88621 acgccgggta atggcattta tgcgtctggc tggcggtcat gcggttgatt tcagcgataa
    88681 taaagatgca tttattaacg tgaatacgcc agaggagctt gcccgatggc aggaaaaacg
    88741 atgataccgt tactcgcctt tgccgcgtgg agtggtaccg gaaaaactac gcttttgaaa
    88801 caactgatcc cggcattatg cgccagaggg atccgtccag ggctgattaa gcatacgcac
    88861 catgatatgg atgttgataa gccaggcaaa gatagctatg agctgcgcaa ggctggcgcg
    88921 gcacaaacca tcgttgccag ccagcaacga tgggccttga tgacggaaac accagacgaa
    88981 gaagagctgg atctacagtt tctcgcaagt cgaatggata cctcaaagct ggatttgatt
    89041 ctggtcgaag ggtttaagca tgaagagatc gcaaagattg tgctgtttcg cgatggagcc
    89101 ggacatcgac ctgaagaatt agtgatagac aggcatgtta ttgctgtagc cagtgatgtg
    89161 ccgcttaatc ttgatgtcgc attactggat attaatgatg ttgaggggct ggctgatttt
    89221 gtggtggagt ggatgcaaaa gcagaacgga taagcaagag taggctggat aagacgcgcc
    89281 agcgtcgcat ccggcatttt tttctgcgcc aatgcaaaaa ggccatcctg acggatggcc
    89341 ttctgcttaa tttgatgcct ggcacttccc tactctcgca tggggagacc ccacactacc
    89401 atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga ccaccgcgct
    89461 a
//
LOCUS       DAZGTR010000005        86926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGTR010000005 DAZGTR010000000
VERSION     DAZGTR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.005"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHLCY_000374"
     CDS             complement(<1..209)
                     /locus_tag="ACHLCY_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0367698.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHLCY_000375"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHLCY_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP0367699.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHLCY_000376"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHLCY_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP0367700.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHLCY_000377"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHLCY_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP0367701.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHLCY_000378"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHLCY_000378"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP0367702.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHLCY_000379"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHLCY_000379"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP0367703.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHLCY_000380"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHLCY_000380"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP0367704.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHLCY_000381"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHLCY_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0367705.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHLCY_000382"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHLCY_000382"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0367706.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHLCY_000383"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHLCY_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP0367707.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHLCY_000384"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHLCY_000384"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0367708.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHLCY_000385"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHLCY_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP0367709.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIIECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9486)
                     /gene="kefB"
                     /locus_tag="ACHLCY_000386"
                     /pseudo
     CDS             complement(7682..9486)
                     /gene="kefB"
                     /locus_tag="ACHLCY_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9486..10006)
                     /gene="kefG"
                     /locus_tag="ACHLCY_000387"
                     /pseudo
     CDS             complement(9486..10006)
                     /gene="kefG"
                     /locus_tag="ACHLCY_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
     gene            10132..12045
                     /gene="yheS"
                     /locus_tag="ACHLCY_000388"
     CDS             10132..12045
                     /gene="yheS"
                     /locus_tag="ACHLCY_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0367710.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12045..13067
                     /gene="yheT"
                     /locus_tag="ACHLCY_000389"
     CDS             12045..13067
                     /gene="yheT"
                     /locus_tag="ACHLCY_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0367711.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGSSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13061..13279
                     /gene="yheU"
                     /locus_tag="ACHLCY_000390"
     CDS             13061..13279
                     /gene="yheU"
                     /locus_tag="ACHLCY_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP0367712.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13333..14202
                     /gene="prkB"
                     /locus_tag="ACHLCY_000391"
     CDS             13333..14202
                     /gene="prkB"
                     /locus_tag="ACHLCY_000391"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP0367713.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14257..14661)
                     /gene="yhfA"
                     /locus_tag="ACHLCY_000392"
     CDS             complement(14257..14661)
                     /gene="yhfA"
                     /locus_tag="ACHLCY_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP0367714.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAV"
     gene            14963..15595
                     /gene="crp"
                     /locus_tag="ACHLCY_000393"
     CDS             14963..15595
                     /gene="crp"
                     /locus_tag="ACHLCY_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP0367715.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15646..17736
                     /gene="yhfK"
                     /locus_tag="ACHLCY_000394"
     CDS             15646..17736
                     /gene="yhfK"
                     /locus_tag="ACHLCY_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP0367716.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17803..19023)
                     /gene="argD"
                     /locus_tag="ACHLCY_000395"
                     /gene_synonym="dapC"
     CDS             complement(17803..19023)
                     /gene="argD"
                     /locus_tag="ACHLCY_000395"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP0367717.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19109..19672)
                     /gene="pabA"
                     /locus_tag="ACHLCY_000396"
     CDS             complement(19109..19672)
                     /gene="pabA"
                     /locus_tag="ACHLCY_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP0367718.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19704..20306)
                     /gene="fic"
                     /locus_tag="ACHLCY_000397"
     CDS             complement(19704..20306)
                     /gene="fic"
                     /locus_tag="ACHLCY_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP0367719.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20296..20463)
                     /gene="yhfG"
                     /locus_tag="ACHLCY_000398"
     CDS             complement(20296..20463)
                     /gene="yhfG"
                     /locus_tag="ACHLCY_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP0367720.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20568..21140)
                     /gene="ppiA"
                     /locus_tag="ACHLCY_000399"
                     /gene_synonym="rotA"
     CDS             complement(20568..21140)
                     /gene="ppiA"
                     /locus_tag="ACHLCY_000399"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP0367721.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21411..22592
                     /gene="tsgA"
                     /locus_tag="ACHLCY_000400"
     CDS             21411..22592
                     /gene="tsgA"
                     /locus_tag="ACHLCY_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP0367722.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22854..25397
                     /gene="nirB"
                     /locus_tag="ACHLCY_000401"
     CDS             22854..25397
                     /gene="nirB"
                     /locus_tag="ACHLCY_000401"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP0367723.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25394..25720
                     /gene="nirD"
                     /locus_tag="ACHLCY_000402"
     CDS             25394..25720
                     /gene="nirD"
                     /locus_tag="ACHLCY_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP0367724.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25846..26652
                     /gene="nirC"
                     /locus_tag="ACHLCY_000403"
     CDS             25846..26652
                     /gene="nirC"
                     /locus_tag="ACHLCY_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP0367725.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26671..28044
                     /gene="cysG"
                     /locus_tag="ACHLCY_000404"
     CDS             26671..28044
                     /gene="cysG"
                     /locus_tag="ACHLCY_000404"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP0367726.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRTGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQKMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28291..28458
                     /gene="yhfL"
                     /locus_tag="ACHLCY_000405"
     CDS             28291..28458
                     /gene="yhfL"
                     /locus_tag="ACHLCY_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP0367727.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28753..30090
                     /gene="frlA"
                     /locus_tag="ACHLCY_000406"
     CDS             28753..30090
                     /gene="frlA"
                     /locus_tag="ACHLCY_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP0367728.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30111..31133
                     /gene="frlB"
                     /locus_tag="ACHLCY_000407"
     CDS             30111..31133
                     /gene="frlB"
                     /locus_tag="ACHLCY_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP0367729.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGQLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31183..32013
                     /gene="frlC"
                     /locus_tag="ACHLCY_000408"
     CDS             31183..32013
                     /gene="frlC"
                     /locus_tag="ACHLCY_000408"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP0367730.1"
                     /translation="MKTGMFTCGHQRLPIEHVFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32010..32795
                     /gene="frlD"
                     /locus_tag="ACHLCY_000409"
     CDS             32010..32795
                     /gene="frlD"
                     /locus_tag="ACHLCY_000409"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP0367731.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNMAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSSLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32895..33626
                     /gene="frlR"
                     /locus_tag="ACHLCY_000410"
                     /gene_synonym="yhfR"
     CDS             32895..33626
                     /gene="frlR"
                     /locus_tag="ACHLCY_000410"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP0367732.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33778..34863)
                     /gene="yhfS"
                     /locus_tag="ACHLCY_000411"
     CDS             complement(33778..34863)
                     /gene="yhfS"
                     /locus_tag="ACHLCY_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0367733.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34875..36179)
                     /gene="yhfT"
                     /locus_tag="ACHLCY_000412"
     CDS             complement(34875..36179)
                     /gene="yhfT"
                     /locus_tag="ACHLCY_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP0367734.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36191..36544)
                     /gene="yhfU"
                     /locus_tag="ACHLCY_000413"
     CDS             complement(36191..36544)
                     /gene="yhfU"
                     /locus_tag="ACHLCY_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP0367735.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAAFSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36555..37433)
                     /gene="php"
                     /locus_tag="ACHLCY_000414"
     CDS             complement(36555..37433)
                     /gene="php"
                     /locus_tag="ACHLCY_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP0367736.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37430..38656)
                     /gene="yhfW"
                     /locus_tag="ACHLCY_000415"
     CDS             complement(37430..38656)
                     /gene="yhfW"
                     /locus_tag="ACHLCY_000415"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0367737.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38656..39819)
                     /gene="yhfX"
                     /locus_tag="ACHLCY_000416"
     CDS             complement(38656..39819)
                     /gene="yhfX"
                     /locus_tag="ACHLCY_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP0367738.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPMLAEYGVTHAEPGHALTGTIPANQQGDQPEYIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(39903..40265)
                     /gene="yhfY"
                     /locus_tag="ACHLCY_000417"
     CDS             complement(39903..40265)
                     /gene="yhfY"
                     /locus_tag="ACHLCY_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0367739.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40282..41187)
                     /gene="yhfZ"
                     /locus_tag="ACHLCY_000418"
     CDS             complement(40282..41187)
                     /gene="yhfZ"
                     /locus_tag="ACHLCY_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP0367740.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41457..42461)
                     /gene="trpS"
                     /locus_tag="ACHLCY_000419"
     CDS             complement(41457..42461)
                     /gene="trpS"
                     /locus_tag="ACHLCY_000419"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP0367741.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42454..43212)
                     /gene="gph"
                     /locus_tag="ACHLCY_000420"
     CDS             complement(42454..43212)
                     /gene="gph"
                     /locus_tag="ACHLCY_000420"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP0367742.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43205..43882)
                     /gene="rpe"
                     /locus_tag="ACHLCY_000421"
     CDS             complement(43205..43882)
                     /gene="rpe"
                     /locus_tag="ACHLCY_000421"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP0367743.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43900..44736)
                     /gene="dam"
                     /locus_tag="ACHLCY_000422"
     CDS             complement(43900..44736)
                     /gene="dam"
                     /locus_tag="ACHLCY_000422"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0367744.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44843..46129)
                     /gene="damX"
                     /locus_tag="ACHLCY_000423"
     CDS             complement(44843..46129)
                     /gene="damX"
                     /locus_tag="ACHLCY_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP0367745.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46221..47309)
                     /gene="aroB"
                     /locus_tag="ACHLCY_000424"
     CDS             complement(46221..47309)
                     /gene="aroB"
                     /locus_tag="ACHLCY_000424"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP0367746.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47366..47887)
                     /gene="aroK"
                     /locus_tag="ACHLCY_000425"
     CDS             complement(47366..47887)
                     /gene="aroK"
                     /locus_tag="ACHLCY_000425"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP0367747.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48288..49526)
                     /gene="hofQ"
                     /locus_tag="ACHLCY_000426"
     CDS             complement(48288..49526)
                     /gene="hofQ"
                     /locus_tag="ACHLCY_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP0367748.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49438..49842)
                     /gene="hofP"
                     /locus_tag="ACHLCY_000427"
     CDS             complement(49438..49842)
                     /gene="hofP"
                     /locus_tag="ACHLCY_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP0367749.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49832..50272)
                     /gene="hofO"
                     /locus_tag="ACHLCY_000428"
     CDS             complement(49832..50272)
                     /gene="hofO"
                     /locus_tag="ACHLCY_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP0367750.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50256..50795)
                     /gene="hofN"
                     /locus_tag="ACHLCY_000429"
     CDS             complement(50256..50795)
                     /gene="hofN"
                     /locus_tag="ACHLCY_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP0367751.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50795..51574)
                     /gene="hofM"
                     /locus_tag="ACHLCY_000430"
     CDS             complement(50795..51574)
                     /gene="hofM"
                     /locus_tag="ACHLCY_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP0367752.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51694..54246
                     /gene="mrcA"
                     /locus_tag="ACHLCY_000431"
     CDS             51694..54246
                     /gene="mrcA"
                     /locus_tag="ACHLCY_000431"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP0367753.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54411..54971)
                     /gene="nudE"
                     /locus_tag="ACHLCY_000432"
     CDS             complement(54411..54971)
                     /gene="nudE"
                     /locus_tag="ACHLCY_000432"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP0367754.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55291..57426
                     /gene="igaA"
                     /locus_tag="ACHLCY_000433"
     CDS             55291..57426
                     /gene="igaA"
                     /locus_tag="ACHLCY_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP0367755.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57491..58159
                     /gene="yrfG"
                     /locus_tag="ACHLCY_000434"
     CDS             57491..58159
                     /gene="yrfG"
                     /locus_tag="ACHLCY_000434"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP0367756.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58170..58571
                     /gene="hslR"
                     /locus_tag="ACHLCY_000435"
     CDS             58170..58571
                     /gene="hslR"
                     /locus_tag="ACHLCY_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP0367757.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58596..59474
                     /gene="hslO"
                     /locus_tag="ACHLCY_000436"
     CDS             58596..59474
                     /gene="hslO"
                     /locus_tag="ACHLCY_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP0367758.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILTEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59609..61333)
                     /gene="yhgE"
                     /locus_tag="ACHLCY_000437"
     CDS             complement(59609..61333)
                     /gene="yhgE"
                     /locus_tag="ACHLCY_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP0367759.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYKSVWHNVLILLVIFLANGLTWVVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61711..63333
                     /gene="pckA"
                     /locus_tag="ACHLCY_000438"
     CDS             61711..63333
                     /gene="pckA"
                     /locus_tag="ACHLCY_000438"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP0367760.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63409..64761)
                     /gene="envZ"
                     /locus_tag="ACHLCY_000439"
     CDS             complement(63409..64761)
                     /gene="envZ"
                     /locus_tag="ACHLCY_000439"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP0367761.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64758..65477)
                     /gene="ompR"
                     /locus_tag="ACHLCY_000440"
     CDS             complement(64758..65477)
                     /gene="ompR"
                     /locus_tag="ACHLCY_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP0367762.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65705..66181
                     /gene="greB"
                     /locus_tag="ACHLCY_000441"
     CDS             65705..66181
                     /gene="greB"
                     /locus_tag="ACHLCY_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP0367763.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66278..68599
                     /gene="yhgF"
                     /locus_tag="ACHLCY_000442"
     CDS             66278..68599
                     /gene="yhgF"
                     /locus_tag="ACHLCY_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP0367764.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVMSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69037..69264
                     /gene="feoA"
                     /locus_tag="ACHLCY_000443"
     CDS             69037..69264
                     /gene="feoA"
                     /locus_tag="ACHLCY_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP0367765.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69281..71602
                     /gene="feoB"
                     /locus_tag="ACHLCY_000444"
     CDS             69281..71602
                     /gene="feoB"
                     /locus_tag="ACHLCY_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP0367766.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71602..71838
                     /gene="feoC"
                     /locus_tag="ACHLCY_000445"
     CDS             71602..71838
                     /gene="feoC"
                     /locus_tag="ACHLCY_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP0367767.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72041..72919
                     /gene="rpnA"
                     /locus_tag="ACHLCY_000446"
     CDS             72041..72919
                     /gene="rpnA"
                     /locus_tag="ACHLCY_000446"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP0367768.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(72948..73718)
                     /gene="bioH"
                     /locus_tag="ACHLCY_000447"
     CDS             complement(72948..73718)
                     /gene="bioH"
                     /locus_tag="ACHLCY_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP0367769.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            73756..74439
                     /gene="gntX"
                     /locus_tag="ACHLCY_000448"
     CDS             73756..74439
                     /gene="gntX"
                     /locus_tag="ACHLCY_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP0367770.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            74498..75073
                     /gene="nfuA"
                     /locus_tag="ACHLCY_000449"
     CDS             74498..75073
                     /gene="nfuA"
                     /locus_tag="ACHLCY_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP0367771.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            75434..76750
                     /gene="gntT"
                     /locus_tag="ACHLCY_000450"
     CDS             75434..76750
                     /gene="gntT"
                     /locus_tag="ACHLCY_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0367772.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(76795..78879)
                     /gene="malQ"
                     /locus_tag="ACHLCY_000451"
     CDS             complement(76795..78879)
                     /gene="malQ"
                     /locus_tag="ACHLCY_000451"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP0367773.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(78889..81282)
                     /gene="malP"
                     /locus_tag="ACHLCY_000452"
     CDS             complement(78889..81282)
                     /gene="malP"
                     /locus_tag="ACHLCY_000452"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP0367774.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            81894..84599
                     /gene="malT"
                     /locus_tag="ACHLCY_000453"
     CDS             81894..84599
                     /gene="malT"
                     /locus_tag="ACHLCY_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP0367775.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(84642..85658)
                     /gene="rtcA"
                     /locus_tag="ACHLCY_000454"
     CDS             complement(84642..85658)
                     /gene="rtcA"
                     /locus_tag="ACHLCY_000454"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP0367776.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(85662..86888)
                     /gene="rtcB"
                     /locus_tag="ACHLCY_000455"
     CDS             complement(85662..86888)
                     /gene="rtcB"
                     /locus_tag="ACHLCY_000455"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP0367777.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT        20739 a        23754 c        21948 g        20485 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agaaaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtatcag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaataata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcga taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcagatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagagc aatcctttga agggatcgat
     8701 agccgtttcc agttcatggc gatattcact ttccgccagc agcacgcccg caataaatgt
     8761 accgagcgcc atcgacagcc ccagcgcatc cataaacaat gcggaaccca acaccagcag
     8821 cagcgtcgcg gcggtgaaca cttcccgcac gccagaagct gcaataaagc ggaataccgg
     8881 acgcagtaaa tagcgcccac caatcagcat gccgacaaac gccagcacct tcatgccgat
     8941 cttcatccag tcgaaatgtt cgtctgccga acccgccaac aacggcacta acgccagtgc
     9001 cgggatcacc gccagatcct gaaacagcag taccgaaaac ccgagctgcc cggattcgct
     9061 gcgattcatc cctttctcac gcatcaattg caacgccatt gcggtcgaag acatcgcaag
     9121 gccaatgcca ccgaccaccg ccgcctgcca ggcgaaatcc gtcagcatca ataatcccgc
     9181 cagcaacgcc gcgcttaaca gcacctgtgc cgcgcctacg ccaaaaatcg aacgccgcag
     9241 ttgccaaagc ttggagggat tcaactcaag gccgatgata aacatcagga ataccacgcc
     9301 gagttccgaa aagtggagga tctcatcgac gtcgctaata aaccccagcc cccacgggcc
     9361 aattgcaatc cctgccagca aatatcccaa cacagcgcca atccccagcc gcgatgccaa
     9421 cggcaccgca gccaccgccg cgaagagaaa cagcactcct gcgagtaaaa aatcggaacc
     9481 ttccatcagc ggcctcctgg agacagcgga tttgccagcc agtcaccgta ggctctggcg
     9541 tggctcgcca gctcctgtgc gctttgccgt ctcgcccagt aaataatgat gggacttaac
     9601 caatgcatcc ggcacatgcc cgccgccagt tcaaagggac gcagaacgtc gctcatgggg
     9661 tagcgattca atgcgtcata acggtaagca ctttccggct cgccggtggt gatcacgcta
     9721 cgccagtact ttcccgccag ttggtttcct cccggtccag ccactctttc agtagcgccg
     9781 ggcagctata ggtataaaga ggatgctgaa agacaatcac ctcgtgctcg cgcagtaatg
     9841 cctgctcacg ggggatatca ataaaaaaat cgggatagtg cgcataaagg tcgtgcacgg
     9901 taacattgct gagctgcgtg gccggtttaa gcagtacccg gtttgccacc gagtcctgag
     9961 attccggatg ggcaaacagc agcaaaactt tcgctggctg agacatcatc cccctcccgg
    10021 tatggttttt gtgtatagtc gctgttttgg gctaccattg cgcccggtgc ggcagctcgc
    10081 ccatacatta tcataatgat aagttaacat agtctgaaca tacggcacct tatgattgtt
    10141 ttctcctcgt tacaaattcg tcgcggcgtg cgcgtcctgc tggataatgc caccgccacc
    10201 atcaaccctg ggcagaaagt cggcctggtg ggtaaaaacg gctgtggtaa atctaccctg
    10261 ctggcattgc tgaaaaatga aatcagcgcc gacggcggca gctacacctt tccgggaagc
    10321 tggcaactgg cgtgggtgaa tcaggaaacg ccggcgttac cgcaagcggc gctggaatat
    10381 gtcattgacg gcgaccgtga atatcgtcaa ctagaagcgc agctacacga cgccaacgaa
    10441 cgtaacgacg ggcacgccat tgcgaccatt catggcaagc tggatgctat tgacgcatgg
    10501 agtattcgct cccgtgccgc cagcctgctg cacggcctcg gtttcagcaa tgaacaactg
    10561 gagcgcccgg taagtgattt ctccggtggc tggcgtatgc gtcttaacct tgcccaggcg
    10621 ctgatttgcc gttcagactt gctgctgctc gacgaaccga ctaaccacct cgatctcgat
    10681 gccgttatct ggctggaaaa atggctgaag agctatcagg gcacgctgat cctgatctct
    10741 cacgaccgcg acttcctcga tccgatcgtc gataaaatta ttcatatcga acaacaaagc
    10801 atgttcgagt acaccggcaa ctacagttcg tttgaagtac agcgcgccac ccgtctggcg
    10861 cagcaacaag cgatgtacga aagccagcag gaacgcgtag cgcatctgca aagttatatc
    10921 gaccgtttcc gtgccaaagc caccaaagcg aagcaggccc agagccgcat taagatgctc
    10981 gagcgtatgg agctaattgc ccccgcgcac gtcgacaacc cgttccgctt tagcttccgc
    11041 gcgccggaaa gcctgccaaa tccgttactg aagatggaaa aagtcagcgc gggctatggc
    11101 gatcgcatta ttctcgactc gattaaactg aacctggtgc ccggctcgcg cattggtctg
    11161 ttaggccgca atggcgcggg taaatcgaca ttaatcaaac tgttagccgg tgaacttgcg
    11221 ccagtcagcg gtgaaattgg tctggcgaaa gggatcaaac tcggctactt cgcccagcat
    11281 caacttgaat acctgcgcgc cgacgaatca cctattcaac atctggcacg tttagcgccg
    11341 caggagctgg aacaaaaact gcgtgactac ctcggcggct ttggtttcca gggcgataaa
    11401 gtaaccgaag aaacgcgccg cttctccggt ggggaaaaag cccgcctggt gctggcatta
    11461 attgtctggc agcggccgaa tctgctgctg ctcgacgaac cgactaacca ccttgacctc
    11521 gacatgcgtc aggcactcac cgaagcatta atcgagtttg aaggcgcgct ggttgtcgtt
    11581 tcgcacgacc gtcatttgct gcgttccacc actgacgatc tctacctggt tcacgatcgt
    11641 aaagtcgaac cgttcgacgg cgatctggaa gattatcaac agtggttgag cgacgtacaa
    11701 aagcaggaaa accagaccga cgaagcgcca aaagagaatg cgaacagcgc ccaggcacgt
    11761 aaagatcaga agcgtcggga agctgagctg cgtgcgcaaa cccagccact gcgtaaagag
    11821 attgcccgtc tggaaaaaga gatggagaag ctgaacgcgc aactggcgca ggcggaagag
    11881 aaactcggcg acagcgaact gtatgaccag agccgtaaag cggagttgac cgcctgcctg
    11941 caacagcaag ccagcgccaa atccggcctg gaagagtgcg aaatggcatg gctggaagcc
    12001 caggagcagc ttgagcagat gctgctggaa ggccaaagca actgatggcg cagataacga
    12061 cgaccgatgc caatgaattc agcagcagtg ctgaattcat ccccatgcgc ggctctagca
    12121 attgtcatct gcaaaccatg ctgccgcgtc tgtttcgtcg tcaggtgaaa ttcaccccgt
    12181 actggcagcg gctggagttg cccgacggcg attttgtcga tcttgcgtgg agtgaaaacc
    12241 ctgcacaggc gcaacataaa cctcgtctgg tggtgtttca cgggctggaa ggcagtctca
    12301 acagccctta cgcccacggt ctggttgagg cggcgcaaaa gcgcggctgg ctgggcgtgg
    12361 tgatgcattt tcgcggatgc agcggtgaac caaaccgtat gcaccgcatt taccattcgg
    12421 gcgaaaccga agacgccagt tggtttttac gctggctgca acgcgaattt ggtcatgcgc
    12481 caacggctgc cgtcggctat tcgctcggcg gtaatatgct ggcctgtttg ctggcaaagg
    12541 aaggcaatga tctcccggtt gatgcggcgg tgattgtctc tgcgccgttt atgctggaag
    12601 cctgtagcta tcatatggaa aagggctttt cccgcgttta tcagcgttac ttgctgaacc
    12661 tgttaaaagc caatgccgcg cgcaagctgg cagcctaccc cggaacgctg ccgattaatc
    12721 tcgcacagtt aaaatcggta cgtcgcatcc gtgaatttga cgatctcatc accgccagaa
    12781 ttcacggcta cgccgacgct atcgactatt atcgtcagtg tagcgccatg ccgatgctga
    12841 accggatcgc caaaccgacg ctgattattc acgccaaaga cgatccgttt atggatcatc
    12901 aggtgatccc gaaaccggaa agtctccccc cgcaggtgga gtatcaactg actgaacatg
    12961 gcggtcatgt tggctttatt ggcggtacat tacttcatcc gcaaatgtgg ctggagtcac
    13021 gcattcctga ctggttaaca acgtatctgg aggcgaaatc atgttgattc cgtggcaaga
    13081 cctctccccc gaaacgctgg aaaatttaat tgaaagcttt gtgttacgtg aaggcaccga
    13141 ttatggtgaa catgagcgta cacttgaaca gaaagtcgcc gacgtcaaac gccagctaca
    13201 gtgcggagaa gcggtgctgg tatggtcgga gctgcacgaa acggtcaata ttatgccgcg
    13261 cagtcagttt cgcgaataac ctgcaaccgt cggcggacga gataataatg atagctaagt
    13321 catggagtta ctatgtctgc caaacatccg gtcattgcgg taacaggatc cagcggcgcg
    13381 gggaccacca ccaccagcct cgcgtttcgt aaaatattcg cgcagttaaa tctgcatgca
    13441 gctgaggtgg aaggcgacag ttttcaccgt tacacccgcc cggaaatgga catggcgatc
    13501 cgcaaagcgc gcgacgccgg gcggcatatc agctacttcg gccccgaggc taacgacttc
    13561 ggcctgctgg aacaaacctt cattgaatac ggtcagagcg gcaaagggaa atctcgcaaa
    13621 tatctgcata cctacgacga agccgtaccg tggaatcagg taccggggac attcaccccc
    13681 tggcaacctt taccggaacc cactgatgta ctgttttatg aaggtttaca cggcggcgta
    13741 gtcacgccac agcataacgt tgcgcagcat gtggacttac tggtcggcgt ggtgcctatc
    13801 gttaaccttg agtggattca aaaactgatc cgcgacacca gcgagcgcgg gcactcacga
    13861 gaagcagtga tggactcagt agtgcgttca atggaagact atatcaacta catcacaccg
    13921 cagttttccc gcacccatct taacttccag cgcgttccca ccgtcgacac ttcaaacccg
    13981 ttcgcggcaa aaggtatccc gtcgctcgat gaaagctttg tggtgatcca ttttcgtaat
    14041 ctggaaggga tcgatttccc ctggctgctg gcgatgttgc aaggctcatt catttcccac
    14101 atcaatacgt tagtggtacc gggcggcaaa atgggtctgg caatggaatt aattatgctg
    14161 ccgctggtgc aacgattgat ggaaggaaag aaaatcgagt aactctgcta ttacgccgga
    14221 taaaatacta tccggcttca caacgggata gttaagtcac acggcaacca cttcatacga
    14281 gtgagtaata ttcaccgctt tttccagcat caacgccact gaacaatatt tctcggcaga
    14341 gagatcaacc gcacgcgcaa ccgctgcgtc tttcaggtcg cgaccggtga cgataaaatg
    14401 cagattaatg tgcgtaaaca ggcgtggtgc ctcttcgcgg cgttcagagg tcaattttac
    14461 ttcacaatcg accacatcct gacgcccttt ttgcaggatc gaaaccacat cgatggcact
    14521 gcaaccaccc gccgccatca acaccatttc catcggactt ggtgctttat cgcctgagtt
    14581 gccgtccatt aaaatctgat gaccagaggc ggattcgccc agaaaagtta acccttcgac
    14641 ccacttcact cgcgcttgca tttttgctac tccactgcgt caattttcct gacagagtac
    14701 gcgtactaac caaatcgcgc aacggaaggc gacctgggtc atgctgaagc gagacaccag
    14761 gagacacaaa gcgaaagcta tgctaaaaca gtcaggatgc tacagtaata cattgatgta
    14821 ctgcatgtat gcaaaggacg tcacattacc gtgcagtaca gttgatagcc ccttcccagg
    14881 tagcgggaag catatttcgg caatccagag acagcggcgt tatctggctc tggagaaagc
    14941 ttataacaga ggataaccgc gcatggtgct tggcaaaccg caaacagacc cgactctcga
    15001 atggttcttg tctcattgcc acattcataa gtacccatcc aagagcacgc ttattcacca
    15061 gggtgaaaaa gcggaaacgc tgtactacat cgttaaaggc tctgtggcag tgctgatcaa
    15121 agacgaagag ggtaaagaaa tgatcctctc ctatctgaat cagggtgatt ttattggcga
    15181 actgggcctg tttgaagagg gccaggaacg tagcgcatgg gtacgtgcga aaaccgcctg
    15241 tgaagtggct gaaatttcgt acaaaaaatt tcgccaattg attcaggtaa acccggacat
    15301 tctgatgcgt ttgtctgcac agatggcgcg tcgtctgcaa gtcacttcag agaaagtggg
    15361 caacctggcg ttcctcgacg tgacgggccg cattgcacag actctgctga atctggcaaa
    15421 acaaccagac gctatgactc acccggacgg tatgcaaatc aaaattaccc gtcaggaaat
    15481 tggtcagatt gtcggctgtt ctcgtgaaac cgtgggacgc attctgaaga tgctggaaga
    15541 tcagaacctg atctccgcac acggtaaaac catcgtcgtt tacggcactc gttaatcccg
    15601 tcggagtggc gcgttacctg gtagcgcgcc attttgtttc ccccgatgtg gcgcagactg
    15661 atttatcacc ccgatatcaa ctatgcactt cgacaaacgc tggtgctatg tttgcccgtg
    15721 gccgttgggt taatgcttgg cgaattacga ttcggtctgc tcttctctct cgttcctgcc
    15781 tgttgcaata ttgcgggcct tgatacgcct cataaacgtt ttttcaaacg cttaatcatt
    15841 ggtgcgtcgc tgtttgccac ctgtagctta ctgacacagc tgctactggc aaaagatgtt
    15901 cccctgccct ttttgctgac cggattaacg ctggtacttg gcgtcactgc tgagttgggg
    15961 ccattgcacg caaaattgct tcccgcatcg ctgctggcag ccatttttac cctcagtctg
    16021 gcgggataca tgccggtctg ggaaccgttg ctcatctatg cgttgggcac tctctggtac
    16081 ggattgttta actggttttg gttctggatc tggcgcgaac aaccgctgcg cgagtcacta
    16141 agtctgctgt accgtgaact ggcagattat tgtgaagcca aatacagcct gcttacccag
    16201 cacaccgacc ctgaaaaagc gctgccgccg ctgctggtgc gccagcaaaa agcggtcgat
    16261 ctaattaccc agtgctatca gcaaatgcat atgctttccg cgcaaaataa tactgactac
    16321 aagcggatgc tgcgtatttt ccaggaggcg ctggatttac aggaacatat ttcggtcagt
    16381 ttgcatcagc cggaagaggt gcaaaagctg gtcgagcgta gccatgcgga agaagttatc
    16441 cgctggaatg cgcaaaccgt cgccgctcgc ctgcgcgtgc tggctgatga cattctttac
    16501 catcgcctgc caacgcgttt tacgatggaa aagcaaattg gcgcactgga aaaaatcgcc
    16561 cgccagcatc cggataatcc ggttgggcaa ttctgctact ggcatttcag ccgcatcgcc
    16621 cgcgtgctgc gcacccaaaa accgctctat gcccgtgact tactggccga taaacagcgg
    16681 cgaatgccat tacttccggc gctgaaaagt tatctgtcac taaagtctcc ggcgctacgc
    16741 aatgccggac gactcagtgt gatgttaagc gttgccagcc tgatgggcac cgcgctgcat
    16801 ctgccgaagt cgtactggat cctgatgacg gtattgctgg tgacacaaaa tggctatggc
    16861 gcaacccgtc tgaggattgt gaatcgctcc gtgggaaccg tggtcgggtt aatcattgcg
    16921 ggcgtggcgc tgcactttaa aattcccgaa ggttacaccc tgacgttgat gctgattacc
    16981 accctcgcca gctacctgat attgcgcaaa aactacggct gggcgacggt cggttttact
    17041 attaccgcag tgtataccct gcaactattg tggttgaacg gcgagcaata catccttccg
    17101 cgtcttatcg ataccattat tggttgttta attgctttcg gcggtactgt ctggctgtgg
    17161 ccgcagtggc agagcgggtt attgcgtaaa aacgcccatg acgctttaga agcctatcag
    17221 gaagcgattc gcttgattct tagcgaggat ccgcaaccta cgccactggc ctggcagcga
    17281 atgcgggtaa atcaggcaca taacactctg tataactcat tgaatcaggc gatgcaggaa
    17341 ccggcgttta acagccatta tctggcagat atgaaactgt gggtaacgca cagccagttt
    17401 attgttgagc atattaatgc catgaccacg ctggcgcggg aacaccgggc attgccacct
    17461 gaactggcac aagagtattt acagtcttgt gaaatcgcca ttcagcgttg tcagcagcga
    17521 ctggagtatg acgaaccggg tagttctggc gatgccaata tcatggatgc gccggagatg
    17581 cagccgcacg aaggcgcggc aggtacgctg gagcagcatt tacagcgggt tattggtcat
    17641 ctgaacacca tgcacaccat ttcgtcgatg gcatggcgtc agcgaccgca tcacgggatt
    17701 tggctgagtc gcaagttgcg ggattcgaag gcgtaatgca ggctaaacca tcggataagg
    17761 cattcacgcc gcatccgaca tcttttgcct gatgcttcgt atttacgccc caaccacctt
    17821 cgccaccgcg tgggcgaaac gttgcatccc ttcatcgata tccgcatctt ccaccaccag
    17881 cgacggtgca aaacgcatca catccggtcc ggcattcagc accattacgc cagcctctgc
    17941 gcccgcatac aggaaatcac gcgcccgacc tttgtactgt ggtttcagct ctgcgccaat
    18001 caacagcccc ataccgcgaa tatcgctaaa cacatcgtac tgctgatcga tcttctgcag
    18061 atggtcaaca aaacgctggc gtttcgcctg aatgccttcc agcacttcag gggtattgat
    18121 gatatcaaac gccgcccccg ctactgcaca ggccagagga ttaccgccgt aggtggaacc
    18181 gtgagaacca ggatgaaacg cagaagcaat ttctgccgtg gtcagcatgg cgctaatcgg
    18241 gaagccgccg cctaacgctt tcgcagaggt cagaatatcc ggcgtcacgc cgtagtgcat
    18301 gtaagcaaac aaatcgccgg tccgccccat cccgcactgt acttcatcaa acaccaataa
    18361 tgcctgatgt tgatcgcaca gctcgcgcaa gccctgcaaa aactctggcg tcgctgccgt
    18421 cacaccgccc tcgccctgga tcggctcaac caccaccgca caggtgtgat catccatcac
    18481 cgctttcact gcgtggagat cgttaaaagg aacgtggatg atgtctgccg gttttggccc
    18541 aaagccgtcg gaatattttg gctgcccgcc caccgagacg gtaaacagcg agcgaccatg
    18601 aaaagcgtta tggaaggcaa taattttggt tttgaacgga ctatgacgca cacaggcgta
    18661 atggcgtgcc agtttaaagg cggtttcgtt agcttccgtg ccggagttca taaacaccac
    18721 gcgttcggca aacgtcgcct caatcagttt gcgcccaaga cgcagcgccg gttcattggt
    18781 gaaaacgtta ctgatatgcc acagagtttc gccctgggtt tttaacgcgt tcaccaacgc
    18841 aggatggcaa tggcccaacg ccgtaactgc aatgccaccc gcgaaatcga catactcctt
    18901 gccttgctga tcccagattc ggctgccctg accttttacc ggaatgaact ctgccggagc
    18961 ataaatcggc aggatcactt catcgaaagt cgcgcgtgta attgctgttt gttcaattgc
    19021 catctcatga tcaccctgtt acgcataaat aaatgtgaaa ttataatcac aaaatatgca
    19081 taaaaaatca ttgaatggca atcagaaatc agcgatgcag gaaattagcc agcagttgat
    19141 gtccttgttc gctaagaata ctttctggat ggaactgcac accttccaga tcccactggc
    19201 gatggcgaat ccccataatc tcgcgggttt cgctccaggc cgtcacgtca aagcatgctg
    19261 gtaatgagcc aggttccacc accagcgaat ggtagcgtgt cacggtaagt ggatttgcca
    19321 gcccccgaaa tacgccctca ccgttatgtg taatcggcga ggttttgccg tgcatgacct
    19381 ttgcggcgcg cacaactttg ccgccaaatg cctgcgccat tgcctgatga ccgaggcaga
    19441 cgccaagaat cggcaagcgc ccggcatagt ggcgaataac gtcaagggag atcccggctt
    19501 catctggcgt acaggggccg ggtgatatga caattttttg tggtttaagg gcgtcgatat
    19561 ccgccagcgt caacgcatcg ttgcgcttaa ccagcacatc cgcccccagt tcacaaaagt
    19621 actggtagag gttccaggta aaagaatcgt agttatctat aagcaggatc atggcggctc
    19681 cggtacgaaa gaaccgcgct attttactca gattccccgg cttcgcttac cactttgcta
    19741 aatatcatct gcagcgcggt gagatccccc attgccccac tctgatttgc ctgattccag
    19801 gcctcttttt cgataccctg ccagctcagt tgatacccgg catgaatcgc cagttgctcg
    19861 aagaagatcc gctgtgccag accacttccc acccggaagg gatgcagcac gttgatttca
    19921 caatagtaat gcgccagccg ctcgacgaac ttcgctttct ccaggccaac cagataacct
    19981 tcttcctcca gatcctgcat cagggcattg ccctcttttt cgatataagc aaagtggcag
    20041 aacggcgtat cgccctgata aatatcaact tcacgcagtt gccccgccca gtcgaaaata
    20101 tcctgataca gctggcgatg gatagtgcgc aaatgcggta aaccgcgcac cagcggacca
    20161 agctcaatgg tcgcagcacg cagcgccgtc atttcgtaag cggcctgttc cagccgctgc
    20221 tgctggtgga tgttcagccg gttacgcatg atatcaaggc caggataaag atacggatcg
    20281 cgcccttcgc cgaatttatc gctcatagtg cctcctcagc tcttcaaggc gcgctaaagc
    20341 ctctgcggca gtaagagtga ctaaaggcat ctcgacgcct tcaagacggc gactggcctg
    20401 gaaattacga ttacgctgaa gctcccagag acgggacttt tgcttatcgg tgagtttctt
    20461 cacttgatgc ctccctgaac gtgtccattt gccacaagta taagcggcaa atccgggtta
    20521 cgccgggaga gcagaagcgc gggcaagatt gcccgcgcga aaaatcatta cggcaggact
    20581 ttagcggaaa ggataactac cggttttgac ggcacattct ggtacggacc aacgtcatga
    20641 gtcggcacct gggaaatctt atcggcaacg tccatgcctt tcaccacttt accaaatacc
    20701 gcgtaaccga aatcacgctg accatggtca aggaaggcgt tatcggcaac gttgataaag
    20761 aactggctgg tggcgctgtc tttgtcagcg gtacgcgcca tcgcgatggt gccacgcgtg
    20821 ttgcgcaggc cgttatcggc ttcatttttg attggcgggt ttggtttttt ctgctgcatc
    20881 tgctcggtga aaccgccgcc ctgaatcata aagccaggaa tgacgcggtg aaaggtagtg
    20941 ttgttataaa aaccgctgtt cacataatcg acaaagtttt gcacagacac tggcgctttt
    21001 tgtttatcca gctccagttc gatgttacca gctgaggttg tcaacaatac gtgcgggtcc
    21061 cctttcgctg ccattgctgc gggagaaaga gcagaaagag cgaaaacagc agccatcgcc
    21121 gccagggtcg atttgaacat gagatttcct taacaaacag agcagaaaaa aagcaagtga
    21181 agagattcta aatagccttc aagaacaagg ccatcccttt acctaatttt acgtatctga
    21241 aatatatgta acctgacgat cactttttat tccgtgatca aaatcacctc ttaaaatgca
    21301 atttagcaac cgattgcaat aaaacattta aacatatcac aaaatcacct aaaatcgccc
    21361 gctcgcggca tatcccgcta tgccatttta tttttgaacg caggccaatc atgactaaca
    21421 gcaatcgcat caagctcaca tggattagct ttctctccta cgcactgact ggtgcgttgg
    21481 ttattgtcac cgggatggtg atgggaaata tcgccgatta tttcaatctg cctgtttcca
    21541 gtatgagtaa caccttcacc ttcctcaacg ccggcatttt aatctctatc ttcctcaacg
    21601 cctggctgat ggaaatcgtc ccgttgaaaa cgcagttacg ttttggcttt ctcctgatgg
    21661 tgctggcggt tgccggtttg atgttcagcc acagcctggc gctgttctcg gcggcgatgt
    21721 tcattctcgg ggtggtcagc ggcatcacca tgtcgattgg tacattcctg gtaacacaaa
    21781 tgtatgaagg gcgtcagcgc ggttcccgcc tgttatttac cgactccttc ttcagtatgg
    21841 ctgggatgat tttcccaatg atcgccgcgt ttctactggc gcgcagcatt gagtggtact
    21901 gggtttatgc ctgcatcggg ctggtgtatg tcgctatttt tattctgacc ttcggctgtg
    21961 agttcccggc gctgggcaaa catgcgccaa aaacggatgc tccggtagag aaagaaaagt
    22021 gggggatcgg cgtactgttt ctctctgttg cggcgctgtg ctacatcctc ggtcagttag
    22081 gttttatctc ctgggtgcct gagtatgcca aaggcctggg catgagcctg aacgacgcgg
    22141 gcacgctggt gagtaacttc tggatgtcat acatggtcgg catgtgggcg ttcagcttta
    22201 ttcttcgctt ctttgatttg caacgcattc tgaccgtact ggctggtctg gctgcgattc
    22261 tgatgtacgt ctttaacacc ggaacaccag cacatatggc gtggtcaatt ctcgctctgg
    22321 gcttcttctc cagcgcgatc tataccacca tcatcaccct gggttcacag cagaccaaag
    22381 taccgtcgcc aaaactggtt aactttgtcc tgacctgcgg aaccatcggt actatgttga
    22441 cctttgtggt taccggcccg attgttgaac atagcggtcc gcaggcggca ctgcttacag
    22501 caaacggtct gtacgctgtc gtctttgtga tgtgcttcct gttaggtttc gtcagccgtc
    22561 accgtcagca taacaccctg acctctcatt aattgctcat gccggacggc actctcgtcg
    22621 tccggccttt tcctctcttc ccccgctacg tgcatctatt tctataaacc cgttcatttt
    22681 gtccattttt tgcacaaaca tgaaatgtca gacaattccg tgacttaaga aaatttatac
    22741 aaatcagcaa tatacccctt aaggagtata taaaggtgaa tttgatttac atcaataagc
    22801 ggggttgctg aatcgttaag gtaggcggta atagaaaaga aatcgaggca aaaatgagca
    22861 aagtcagact cgcaattatc ggtaacggta tggtcggcca tcgctttatc gaagatcttc
    22921 ttgataaatc tgatgcggcc aactttgata ttaccgtttt ctgtgaagaa ccgcgcatcg
    22981 cttatgaccg cgtacacctc tcgtcttact tctctcacca caccgccgaa gagctgtcgc
    23041 tggtgcgcga aggcttctac gagaaacacg gcatcaaagt tctggtcggc gaacgcgcta
    23101 tcaccatcaa ccgtcaggag aaggtgattc actccagcgc cgggcgcacc gttttttatg
    23161 acaagctgat catggcgacc ggttcctacc cgtggatccc gccaatcaaa ggttctgata
    23221 ctcaggactg ctttgtctat cgcactattg aagacctcaa cgccattgaa tcctgcgccc
    23281 gtcgcagcaa acgcggtgcc gttgttggtg gcggcctgtt aggtctggaa gccgctggcg
    23341 cgctgaaaaa cttaggtatt gaaacccacg ttatcgaatt tgcccctatg ctgatggcgg
    23401 aacagcttga tcagatgggt ggcgagcagc tgcgtcgcaa aatcgaaagt atgggcgtgc
    23461 gcgttcacac cagcaaaaac acccttgaga ttgtgcagga aggtgtcgaa gcgcgtaaaa
    23521 ccatgcgttt tgccgacggc agcgaactgg aagtcgactt tatcgtcttc tctaccggta
    23581 tccgtccgcg cgataagctg gcaacccagt gtggtctgga cgttgctccg cgtgggggta
    23641 tcgtcattaa tgattcctgc cagacttccg atccggatat ctacgccatc ggtgaatgcg
    23701 caagctggaa caaccgtgta tttggtctgg tagcacctgg ctacaaaatg gcgcaggtcg
    23761 ccgttgacca tattctcggt agcgaaaacg cctttgaagg tgctgacctt agcgccaagc
    23821 tgaaactgct gggcgtagac gtaggcggta ttggtgatgc gcacggtcgc acgcctggcg
    23881 cacgtagcta cgtttacctc gacgaaagta aagagatcta caaacgcctg attgtcagcg
    23941 aagacaacaa aaccctgctc ggtgcggtac tggtgggcga taccagcgac tacggcaacc
    24001 tgctgcaact ggtgctgaat gctatcgagc tgccggaaaa cccggattct ctgattctgc
    24061 cggcgcactc gggtagcggt aagccgtcta tcggtgttga taaactgccg gacagcgcgc
    24121 aaatctgctc ctgcttcgac gtcaccaaag gtgatctgat tgctgccatc aacaaaggct
    24181 gccacaccgt tgcggcgctg aaagctgaaa ccaaagcggg tactggctgc ggtggctgta
    24241 tcccgctggt tactcaggta ctgaacgcgg aactggcgaa acagggcatc gaagttaaca
    24301 acaacctgtg cgaacacttt gcttattcgc gtcaggaact gttccatttg atccgcgttg
    24361 aaggcattaa aaccttcgaa gaactgctgg cgaaacacgg caaaggctac ggttgtgaag
    24421 tttgtaaacc gaccgtcggt tcgctgctgg cctcctgctg gaacgaatac attctgaagc
    24481 cggaacatac tccgctgcag gattctaacg acaacttcct cgctaacatc cagaaagacg
    24541 gcacctactc ggtgatcccg cgttctccgg gcggtgaaat caccccggaa gggctgatgg
    24601 cagtgggtcg tatcgcgcgt gaatttaatc tctacaccaa aatcaccggc tcccagcgcc
    24661 tggcgatgtt tggcgcacag aaagacgatc tgccggaaat ctggcgtcag ctgattgaag
    24721 ctggcttcga aaccggtcat gcctatgcga aagcactgcg tatggcgaaa acctgcgtgg
    24781 gtagcacttg gtgccgctac ggtgttggcg atagcgtcgg cctcggcgtg gaactggaaa
    24841 accgctacaa aggcatccgt acgccgcaca aaatgaagtt cggtgtctcc ggctgtaccc
    24901 gtgaatgttc agaagctcag ggtaaagacg tgggtattat cgccactgaa aaaggctgga
    24961 acctgtatgt ttgcggtaac ggcggcatga aaccgcgtca tgcggatctg ctggcagcag
    25021 atatcgatcg cgaaacgctg atcaaatatc tcgaccgctt catgatgttc tacatccgta
    25081 ccgccgacaa actgacgcgt accgcgccgt ggttagaaaa cctcgaaggc ggcatcgatt
    25141 acctgaaagc ggtgatcatt gacgacaaac tggggctgaa cgctcatctg gaagaagaga
    25201 tggcgcgcct gcgtgaagcg gtagtgtgtg agtggactga aacggtcaat acaccgtccg
    25261 cgcagactcg cttcaaacac ttcatcaaca gcgacaagcg tgacccgaac gtgcagatgg
    25321 tgccagagcg cgaacagcac cgtccggcaa cgccgtatga acgtatcccg gtaactctgg
    25381 tggaggacaa cgcatgagcc agtggaaaga catctgcaaa atcgatgaca tcctgcctga
    25441 aaccggcgtc tgcgcgctgt taggcgacga gcaggtcgcg attttccgcc cgtatcacag
    25501 cgaccaggtg tttgcgatca gcaacatcga tccgttcttc gagtccagcg tgctgtcacg
    25561 cgggctgatt gcggaacacc agggcgagct gtgggtcgcc agcccgctga aaaaacagcg
    25621 ttttcgctta agtgatggct tgtgcatgga agacgaacag ttttccgtca aacattacga
    25681 agcacgagtg aaagacggcg tggtgcagct gcgcggttaa tgttttaacg ggaggcgcaa
    25741 tgcctcccct ttttgcatgg tcctgtaatg accttcggta taatgcagga cattttttaa
    25801 actttttgtt ttattttttg tttttatttt ttaaaggata atcaaatgtt tacagacact
    25861 attaataagt gtgcggctaa tgctgcacgc attgcacgcc tgtcggcaaa taacccgctc
    25921 ggcttttggg tcagctccgc catggcgggc gcatatgtgg gtcttgggat catcctgatt
    25981 ttcacgctcg gtaatttgct cgacccgtcc gtacgtccgc tggtgatggg cgcgaccttt
    26041 ggtatcgcct taacgctggt gattatcgcc ggttctgaac tgttcaccgg acacaccatg
    26101 ttcctcacct ttggggtaaa agcgggcagc atcagccacg ggcaaatgtg ggcaatcctg
    26161 ccgcaaacct ggctgggtaa cctggtcggt tccgtcttcg ttgccatgct ctatagctgg
    26221 ggcggcggta gcctgctgcc ggtagatacc agcatcgttc actccgtcgc gctggctaaa
    26281 accactgcac cggcaatggt actgttcttc aaaggtgcat tgtgtaactg gctggtttgc
    26341 ctggcaatct ggatggcgct gcgcactgaa ggggcggcga aatttatcgc tatctggtgg
    26401 tgtctgctgg catttatcgc gtccggctac gagcactcta tcgctaacat gacgctgttc
    26461 gcgctctcct ggttcggcaa ccacagcgaa gcctacacgc tggcgggtat tggtcataac
    26521 ctgctgtggg tgacgctggg taatacttta tcaggtgccg tattcatggg attgggttat
    26581 tggtatgcta cgccgaaagc gaatcgtccg gttgcggaca aatttaatca aactgaaacg
    26641 gctgccggtt aattactaag gggtttttac gtggatcatt tgcctatatt ttgccaatta
    26701 cgcgatcgcg actgtctgat tgtcggcggt ggtgatgtcg cggaacgcaa agcaaggttg
    26761 ctgttagacg caggcgctcg cttaacggtg aatgcattag cgtttattcc acagttcacc
    26821 gcatgggcag atgcaggcat gttaaccctc gtcgaagggc catttgatga aagccttctc
    26881 gacacctgct ggctggcgat tgcagcgacg gatgatgaca cgcttaacca gcgcgtcagc
    26941 gaagccgctg aagctcgtcg catcttctgt aacgtagtcg atgcgccgaa agccgccagc
    27001 tttattatgc cgtcgattat tgaccgctca ccgctaatgg tagcggtctc ctctggcggc
    27061 acctctccgg ttctggcgcg cctgttgcgc gaaaaactcg aatcactgct gccgttgcat
    27121 ctgggccagg tggcgaaata tgccgggcaa ttacgcgggc gagtgaaaca acagttcgcc
    27181 acgatgagtg agcgtcgccg tttctgggag aaattgttcg ttaacgaccg cctggcgcag
    27241 tcgctggcaa acaacgatca gaaagccatt actgaaacga ccgaacagtt aatcaacgaa
    27301 ccgctcgacc atcgcggtga agtggtgctg gttggtgcag gtccgggcga tgccgggctg
    27361 ctgacgctga aaggactgca acaaattcag caggcagatg tggtggtcta cgaccgtctg
    27421 gtttctgacg atattatgaa tctggtacgc cgcgatgctg atcgcgtttt cgtcggcaaa
    27481 cgcacgggat accactgtgt accgcaggaa gagattaacc agatcctgct gcgggaagcg
    27541 caaaaaggca aacgcgtggt gcggctgaaa ggtggcgatc cgtttatttt tggccgtggt
    27601 ggcgaagagc tggaaacact gtgcaacgcg ggtattccgt tctcggtggt tccgggtatt
    27661 accgcagctt ctggttgctc tgcctattcg ggtattccgc tcacgcatcg cgattatgcc
    27721 cagagcgtgc gcttaattac cggacactta aaaaccggtg gcgagctgga ctgggaaaac
    27781 ctggcggcag aaaaacagac gctggtgttc tatatggggc tgaatcaggc tgcgactatt
    27841 cagcaaaagc tgattgaaca cggtatgcct ggcgaaatgc cggtggcaat tgtcgaaaac
    27901 ggaacagcag tcacgcagcg cgtgattgac ggtacgctca cgcaactcgg tgaacttgct
    27961 cagaaaatga acagtccatc gctaattatt attggtcggg ttgttggcct gcgcgataaa
    28021 ctgaactggt tctccaacca ttaatttaac ccgggccaga gaattctggc cttcttaatg
    28081 actcttttta tatattcagc aaataaaaca tagcccctta ataaatatta tctgctaatg
    28141 ggttatattc accgttacag cgacttacaa aattaagtca ttcgctcact gacgccaatt
    28201 aaatattcct gtatcgttct taacgagcag tctgcttata ttcatataat caatgaatat
    28261 taattaatat ataatacata gggaatgtaa atgaacaaat ttattaaagt tgcactggta
    28321 ggtgcagtac tggctacgtt aactgcatgt actggtcata ttgaaaaccg tgataagaac
    28381 tgctcttacg actacctgct gcacccggca atttctattt ctaaaatcat tggcggttgc
    28441 ggtcctactg cacagtaagt ctctgacaga aatcggctaa caccggttta attgcccgac
    28501 gccctctgga ttttccgagg gcgtatttgt ttctatgtcc ttcttgcccc cgcccgtaaa
    28561 aatattttca ctttaaattc aatttgataa ctacatccat tagccacagc gcgatcctgt
    28621 gcgaaatttt gtgatcttcc tccacattac ataatatcat atgttgttat attcatcatg
    28681 cattgtcatg ttacctttta aatgactgca aactctcccc tacaacgtat tggacaagaa
    28741 aaaggtatcg ctatgggaag ccaggaactc caacgcaagc tcggattttg ggccgttctt
    28801 gcaatcgccg tcgggacaac cgtcggctcc ggtatttttg tatctgtggg tgaagtggca
    28861 aaagcagcgg gcacgccgtg gcttacggtg ctcgcgtttg tcattggcgg gttaattgtg
    28921 atcccgcaaa tgtgcgtcta tgcggaacta tccaccgctt atccggaaaa tggcgcagat
    28981 tatgtttatc tgaaaaatgc cggaagccga ccgctggctt tcctctccgg ctgggccagc
    29041 ttctgggcca acgatgcgcc gtcattgtcg attatggcgc tggcgattgt cagcaatctt
    29101 ggctttttaa cgcctatcga tccgttgctc ggtaaattta tcgccgccgg attaattatc
    29161 gcctttatgt tgctacacct gcgctccgtt gaaggcggcg cagcgtttca gacgctaatt
    29221 accatcgcca aaattatccc gttcactatc gtcattggcc ttgggatctt ctggtttaaa
    29281 gcggagaatt ttgccgcccc taccaccact gcgattggcg caacgggcag ctttatggcg
    29341 ctgctggcgg ggatctctgc caccagttgg tcgtataccg gcatggcctc tatctgttat
    29401 atgaccggcg aaattaaaaa ccccggaaaa accatgccac gagcgctgat tggttcctgt
    29461 ctgctggttc tggtgctcta caccctgctg gcgctggtga tttccggcct gatgcccttc
    29521 gacaaactcg ccaattctga aacgccgatt tccgacgccc tgacctggat ccccgcactc
    29581 ggcagcaccg ctgggatctt tgttgccatc acggcgatga tcgtcattct tggttcgctt
    29641 tccagctgcg tgatgtacca gccgcggctg gaatacgcga tggcgaaaga caacctgttc
    29701 tttaaatgct tcggccatgt gcatccgaaa tacaacacgc cggatgtctc catcatcctg
    29761 caaggggcgc tggggatctt cttcatcttc gtttccgatc tcaccagcct gctgggttat
    29821 ttcaccctgg tgatgtgttt caaaaatacc ctcaccttcg gctccatcat ctggtgtcgt
    29881 aaacgcgacg attacaaacc gctgtggcgt actccggctt tcgggctgat gaccaccctc
    29941 gccattgcgt caagcctcat tctggtcgcc tcaacctttg tctgggcacc gattcccggc
    30001 cttatctgcg ccgtcatcgt tattgctact ggtctgcctg cttacgcctt ctgggcgaag
    30061 cgtagccgcc agctcaacgc tttgtcgtaa tgttatctgg agaaaataaa atgttggata
    30121 ttgataaaag caccgtggac tttctggtca ccgaaaatat ggttcaggaa gtggaaaaag
    30181 ttctcagcca tgacgttccg ctggtgcaca ccatcgtgga agagatggtg aagcgcgaca
    30241 ttgatcgtat ttatttcgtt gcctgcggat cgccactcaa cgcggcgcaa acggcgaaac
    30301 atctggcgga tcgcttttcc gatcttcagg tctacgccat ttccggctgg gagttctgcg
    30361 ataacacccc gtatcgcctc gacgatcgtt gcgcggtgat tggcgtttct gactacggta
    30421 aaacggaaga ggtaatcaaa gcgctggagc tgggccgggc ctgcggcgca ctcactgcgg
    30481 cgttcaccaa acgcgcggac agcccgatta cctcggcggc ggaatttagc attgattatc
    30541 aggccgactg tatctgggaa attcacctgc tgctctgcta cagcgtggtg ctggagatga
    30601 tcacccgcct cgcgccgaac gcggaaatcg gcaagatcaa aaacgatctc aagcagttgc
    30661 cgaatgcgct cggtcaactg gtacgcacct gggaagaaaa aggccgccag cttggtgaac
    30721 tggccagcca gtggccgatg atttataccg ttgctgcggg tccgctgcgt ccgctgggtt
    30781 acaaagaagg cattgtaacg ctgatggaat ttacctggac gcacggctgc gtgattgaga
    30841 gcggagagtt ccgccatggc ccgctggaga ttgtcgaacc gggcgttccg ttcctgttcc
    30901 tgctcggcaa tgatgaaagt cgccacacca ccgaacgcgc cattaacttt gttaaacagc
    30961 gtactgacaa cgtgatcgtc atcgattacg ccgaaatttc gcaagggctg cacccgtggc
    31021 tggcaccgtt cctgatgttc gtgccaatgg agtggctctg ctactacctg tctatttaca
    31081 aagatcacaa cccggatgaa cgccgctatt acggtggtct ggtggaatat taatccctct
    31141 cccggcccgg caacgggccg aatttacgca aggagttacc cgatgaaaac aggtatgttt
    31201 acctgcggcc accagcggct gcctattgaa cacgtatttc gtgatgcaag cgagctgggt
    31261 tacgacggca tcgaaatttg gggcggtcgc ccgcacgcgt tcgcgccgga cttaaaagcg
    31321 ggcggcatca aacaaatcaa ggcgctggcg cagacgtatc agatgccgat tatcggctat
    31381 acgccagaaa ccaacggcta tccgtataac atgatgctgg gcgatgaaca tatgcgtcgc
    31441 gaaagcctcg acatgatcaa gctggcgatg gatatggcaa aagagatgaa cgcgggttat
    31501 acgctgattt ccgcggccca cgcgggctat ctcacgccac ctaatgttat ctggggacgg
    31561 ctggcagaga acctaagcga actgtgtgag tacgcggaaa acatcggcat ggatctaatc
    31621 ctcgaaccgt taacgccgta tgaatcgaac gtcgtgtgta atgctaatga tgtgcttcat
    31681 gcgctggcgc tggtgccttc gccgcgcctg ttcagcatgg tcgacatttg cgcgccgtat
    31741 gtccaggcgg aaccggtgat gagttatttc gacaaactgg gcgataaatt acgtcatctg
    31801 catattgtcg acagcgacgg ggccagcgac acgcattaca ttcctggaga aggcaaaatg
    31861 ccgctgaggg aactgatgcg cgatattatc gaccggggct atgagggtta ctgtacggtg
    31921 gagctggtga cgatgtatat gaacgagccc agactctatg cccgccaggc gctggaacgc
    31981 tttcgcgcgc tgctgccgga ggatgagaga tgaaaaccct ggcgacaatc ggcgataact
    32041 gcgtcgatat ctacccgcaa ctgaataaag cgttttctgg cggtaatgcg gtcaatatgg
    32101 cggtgtactg cactcgctac ggcatacagc cgggatgcat tacctgggtg ggtgacgatg
    32161 actacggcac aaagctgaag caggatctcg cccgcatggg cgtcgatatc agccatgtcc
    32221 atacgaaaca cggcgttacc gcacaaactc aggtggaact gcacgacaat gatcgcgttt
    32281 ttggcgacta caccgaaggc gtgatggccg actttgccct gagtgaagag gattacgcct
    32341 ggctggcgca gtatgacatt gtgcacgcgg caatctgggg acatgcggaa gacgcattcc
    32401 cacagctgca cgctgcgggc aaacttaccg ctttcgactt ctccgacaag tgggacagct
    32461 cgctctggca gacactggtg ccgcatctcg attttgcctt tgcctccgca ccgcaagaag
    32521 acgaagcgct gcgtctgaag atgaaagcga ttgttgcccg tggcgcaggc gtggtgattg
    32581 tcacgctggg tgaaaacggc agcattgcct gggatggcgc gcagttctgg cgtcaggctc
    32641 ctgaaccggt gacggttatc gacaccatgg gtgccggaga ttcgttcatt gccggattcc
    32701 tttgcggctg gtctgcgggg atgacattac cgcaggcgat ggcgcaggga acggcgtgcg
    32761 cggcgaaaac cattcagtac cacggtgcct ggtaggtata acgttggcgt gagcatcttc
    32821 acgccaacgt gctgttactt gccggaaaac gaccctataa tccgagtaat tcattcttta
    32881 tttcagggtc gattatgtca gctacggacc gctactctca tcaactcctc tacgctaccg
    32941 tccgccagcg gttgctggat gatatcgcgc agggggttta ccaggccggg caacagatcc
    33001 ctaccgaaaa cgagctttgt acgcaatata acgtcagccg cattactatt cgcaaagcta
    33061 tcagcgactt agtggcagac ggcgtactga tccgctggca gggcaaaggc acctttgtac
    33121 aaagccagaa ggtcgaaaac gccctgctga ccgtcagtgg ttttaccgat tttggcgtct
    33181 cacaaggcaa ggcgacgaaa gagaaagtga tcgagcagga acgagtcagc gccgcgccgt
    33241 tttgcgaaaa gctgaacatc cctggcaaca gcgaagtgtt ccatctttgc cgggtgatgt
    33301 atctcgataa agagccgctg tttattgata gttcctggat cccactgtcg cgttatcctg
    33361 actttgatga gatttacgtt gaaggaagct ccacttatca gttatttcag gagcgtttcg
    33421 acacgcgagt ggtcagcgac aaaaagacca tcgatatctt tgccgccacc cgcccgcagg
    33481 caaaatggct gaaatgcgaa ctgggcgaac cgttgtttcg catcagcaaa atcgcctttg
    33541 atcagaatga caaaccggtg cacgtctccg aactcttctg ccgcgccaat cgcatcacct
    33601 taacgattga taataaaaga cattaaccgt aggccggata agatgcgcca gcatcgcatc
    33661 cggcgatgct ggcgcgttga attttacatc ccgtacgttc ccctcaccct aaccctctcc
    33721 ccaaaggggc gaggggaccg ttcagtacgg aatttagctt gacggtgcta tccagcatta
    33781 aatactggca atactctcac gcaaaatccg cagcaccgtc tcttcaccgc tgcgattcgg
    33841 gttaatgcga atcgcacaat gttctgattg tggattcgcc tggcgaaacg ttccggaaag
    33901 gcgataaaag agcggcggga tttcatattt cgactctgca cccaccgggt aaggcaaggc
    33961 accgcgcttt tgcgcctctt ccagcactct ggcggcaatc ggctgatgaa actcgacaat
    34021 caacaccttc gactgcgcat tagcaatcac cgcgcttttc acttccggca ccgcaccacc
    34081 gttaagcaaa gccagcaacc gttcagatac ccctgcctgc accgcgtgca tcactggcgc
    34141 aaacaccaga ccacgcaata cttccagcgc ctgtgcgccc tggatctggc taccgccgga
    34201 gtaaagcgtg gcgcgaatac ggttgataac atcagcatcg ccgaccactg caccaacacc
    34261 ctctggccca aatagcttga agcaggaaaa tgtcgagaca ttcgcgccgc attcacagcc
    34321 gattcgcgcc accttcatca ccgcatagtt gtcatcggtt aacgctggaa cacctgccgc
    34381 gcgcaacgtt gccagcacat ctgccagcac gtagctgtcc tgcggctgct ggcgcgtatg
    34441 ctgcaccagc gccgcatccg gttgttgctc gtcgacgacc tgcttcagtg ccgacaggtc
    34501 attgaaatca acagtaataa gcgtcagccc catctgctca ataataaccc gtgtcgtcgg
    34561 gtaaacaggc gcgtcatgca ccagaagacg ctgccccggt ttgagcaaag ccgccagccc
    34621 ggcgcgaatc gcgccagtcc ccgcgccctg caccagcgcc gcagcctgtg cgtgaaatgc
    34681 atcagccagc acctgctcca cccgttgggt aatacgcggt tgattcagac ctggagttaa
    34741 ccctaaatca ccgccagtaa gaaactcgct gccggggaaa tggcgacaaa tgctatccac
    34801 cagcgcaaac tgcttttgct gcgcctcaat aatcgtcagg ctttgcagag gaaacgtctt
    34861 cataggtgcc ttccttaagc cggaacgaac aggccaagcc agtaaagaac attcagcaga
    34921 ataccggtga tcattactgc cacgaccggt gccgccattt tctgtaccgg acggcccagg
    34981 gattcgttga ggaagtaaat ggcaaccgcg atagagaatc cggtataacc cgccatctta
    35041 attgctgcga aaatcgaacc gaccagcagc gccacttcca tcagcatatt catggcgtta
    35101 cggatgttat ccgacgcatt acgcaccgac gggtagcgtc ccagccattt gccgatcgaa
    35161 cgaagcagca agacttccgc cgaaataacc actgcgccta ataccgccgc aaccatcgga
    35221 tttggcgaga gatagcccac cgcataaaca aaggtaaagc ccgcaactgc atacacgcca
    35281 gtggctaacg cggtggtggc aatcaacggc acaaaaccca gtccgcgcat aaattctgcc
    35341 agagccgcct gattaatcag cgtttgcgat tgttccggcg tcacgcctgc ggaatacgct
    35401 ttctccagtg tgaagatcga aacttcgcta ccagcaaaaa tcttcatact ggcaacggcg
    35461 gcaatcaatg ctcccacgat ggcgatataa ggtaagtttt tgataatccg cgacgtgcgc
    35521 tcttcaaaca ccgaaagccc gctggcatcg atgtcatttt catcacgatg acgcaggtcg
    35581 tgagttatcg cgatccccag cagcatcacc atgccaataa agatttcgat ggattcaggg
    35641 ttaagatgtg ggaaatagcg cacgactacc acacgggtca tcagaacaac cacggcggcg
    35701 accagacttt gcttccagcc aaactggtag aaaatcgcca ccagcgggaa cagtgcaaaa
    35761 gcagaaacca ccggcgagct taattccccg aggctaccta atacatccac cggcagcgcg
    35821 gtcagcagct ggtttactgg caacaggcaa gtaaggatca acacgcccca gatagcgcca
    35881 agaccaaacg ccatcaggct gtttatcgcc agtacgccga ggatatcggt aggaagaaag
    35941 agtaaccatg cgttgagcag cccggttttc agggtaaaag agatccccac cgaggccaca
    36001 aaaccaatgc tcagaccaaa agcgatactc cccgcctcgc gacgattcat atagccttca
    36061 atcagttgcg gcaggatcgg gcggatgccg tcatgaaaaa cagccgccga gcgatgcgcc
    36121 agaagcgatg tcatacccgt caggcacgcc accacgataa tctgaatata cagatccata
    36181 gtcgtgcctt ttatttcaga tggttaatca gcatcggaat cgcgtgttcg acgtgctcaa
    36241 cggaaaggcc aaacgccact ttcccttcag cgatcatttt ggcgatatgc tcgtctttcg
    36301 ctttaatgcc tggtttggca atggtgcaac ttttgttata gccaatcacc gcgatggcaa
    36361 tcgaaaatgc cgcgcccgca ccggtattac acgcaccgat gtagtaatcc agttgcccgg
    36421 atttcacttt cattgccgct tccatgtcgt tgtgaatgaa aacttcaaaa ctgccaggag
    36481 ccgtcgcttc aatggttttt ttaatctgct cacgctgtaa gcctgcaacg ccaatctttt
    36541 tcatgagtct gtccttattg gaaaaattga gagggatttt cacgtaacat cacatccaca
    36601 tcggcctgac tgaatcctga ctggcgcaat tgcggaataa aggtggtcaa taaatagtca
    36661 tagccataac caccattggc ttttaaatgg gagcggcgcg taatatccat cgacagcatg
    36721 acgcggttca gcaacccacg gtcacgtagc gcgtggagca tcgcaatgcg cttttcgtcc
    36781 gggtagtaac tgttcttacc aatggtgtcg aactgcacgt acgcgccgag atcgatcatc
    36841 ttcaaaatgt tgtcgagatt gtctttcaga tcgcagtgac caacggtgac gcgcgaaaga
    36901 tcaactccgt gggcttgcaa cagagccagt tgttccagcc ccatggtgct gaacgaagta
    36961 tgcgtggaga tcgggcgtcc ggtctggtta tgcgccagcg cagcggcaat aaacaccttc
    37021 tcttccagcg gcgtaatctt cccttcgcta gagccgatct ccgcgataat cccggctttc
    37081 aggtcggtgc catcgatacc ctgttcaatt tcatcgacca tctcctgcgc cagttcctgc
    37141 acgctgcggg tcgccacatg ctccgggaaa aaagcgtcct ggtaataacc ggtgcaggcc
    37201 accacgttga tccccgtctc gcgcattaca tcgagcataa attgcgcatt gcgccccatg
    37261 taacggttgg tcatctcaat cacattacgc acgccccggg tcatcaggtc gttcatctcc
    37321 tggcaaatga acgcatactg atcaaggcgg cagtccacgt tgtttttaaa gccggagaga
    37381 tcaatatgca gatgctcatg ggccagggtg taacccgtcg gatcaatact catagggtgt
    37441 ctcctgcaaa ccggagggag gaaagaaaag agcgaccatt ttgtggcggt ggcgcacgga
    37501 aaaattcaca cacggtagcc cccacatcag aaagcgtggt gcgtacaccg agttgcgtgt
    37561 ggaccagccc ttgctgataa accagcactg gcaccacttc acgggtatgg tggctgtgac
    37621 caatggtcgg atcgttaccg tgatccgcca tcacgaccag gcaatcatct ggctgcatcg
    37681 cctcaacaag ccgggcaagg ttacggtcaa cgacctgcaa acgttcggca taacgtgcga
    37741 cgtcttctgc atgaccagcg aggtcggttt cctggatatt gatgcaaata aacgccgtcg
    37801 gatgcgtgtt aaattcgtcg agggtgatat ccataatccg ctggctatcc accaggtttt
    37861 gccagctcac gccataagga ttgctgacga tatctgccac cttacctacc agcacggttg
    37921 gcacgcccgc ctcatacagt ttttgcggca cctgcacttt ttcatcaaca ccgtagccca
    37981 tatgcacgac ctggaagccg ttgtcataag cgccagaacg cggcgcatta ataccaataa
    38041 agcgcccttc tttgctttct gcggcatcga gaatacgctg actgtcggtt aacaagccgc
    38101 caaatgtaat gacccgaccg acctgtacct gctcacgcac gatacgacca attttgatcg
    38161 cgtcgtcaaa agagatcaca gagagattgg cggtaatgtt atagacctgg cctaaatccg
    38221 cctcgagatt atcgccaatc gcaaccgcct gattgaccca cagaaattgc agatcatcgc
    38281 cacggcgctc cacctgccag ccagcggaaa ctaatgcctg ctcaacacgg ccaatcacat
    38341 cgcgaaaggg catccgcagc ggcggtaacg ggcgcgtgcc taaaatttcc tgatgcccca
    38401 taaaggtatc gccaccttca tgttgcagct ctgccacccc ccaggttgcg gaatctgacg
    38461 gttgcatatc acctggtgca taacccaatg cgttgattag ccccagcttc tccagcgctg
    38521 gtagctgcaa atgcggcaac tggctcagga tgtgaccaca ggtattcgcc ccggcatctt
    38581 gcgggcgcac cagcgtgaca tctttcattg cccctacgcc aaagctatca atcactaaca
    38641 ccacaaatcg cgccattacg cccccagaga gtttccaaga ctgtcataac gaccgacgat
    38701 tttcggttcg ccacggtgaa taccggacac cagcaccaca tcgctacgag tgacaaaaat
    38761 ctgcgtgcga aaacagagca ctactgcact gcttaccgga aactcgcctg ccagcggcag
    38821 ggtgtagtcg atactgctgt catccacagt tttgagattg gtttcagtaa tcttttgatt
    38881 ttctggcgta aacaccagcg catgttgcgc atgaccacga cggtagtaac cgccgccgta
    38941 gcagtagctg tcgccacgga aatgatggga gatttcactt aaccaaagca tcgctatata
    39001 ttcaggctga tcgccctgct ggtttgccgg aatagtgccc gtcagcgcat gaccgggttc
    39061 ggcatgagtc acaccgtatt ccgccagcat cggcagcgaa gtgcagctgg tcgcagaagg
    39121 cgcgttcagt tgctcaatcg caataccaga tttcgccagt tgatcccgtg cttgtaccag
    39181 cgtgtgaaga ttcggtgtcg gcaaaacttt cccggcagcc tcatcccaaa gcaggcaagg
    39241 gaaatgggta agtccggcta aatgcagacc cggcagtttc ttaatttcag caaccacctc
    39301 gtgcagcgaa tgctgtacaa aaccgctctc ctgccccgga taaagaaaat catcgtcgct
    39361 ataaactttg agcagcacaa actgcacacg tccggccttc accgctgccg cagaaacttc
    39421 ccgcgctttg tcgagagtaa acacggtgat gacgtcggtg ccctgttcaa cggcgtcagc
    39481 aacctgatga caagggattt gtaccagatg cccctgatgc gccaccgaca aaccagcgcg
    39541 gcgcatgact cgcgcctctt tgtaatccac cgccacaatg ccgctgtaac ctaatgccag
    39601 caatttttcc gccagccatg ggttacggcc aaactgcttg gtcatcagat acagttcaat
    39661 gccgtaaagc cgcgccgtct caatcagtcg cttaccgttt tccagtacct gatccacgtc
    39721 gatcacccag ctgtccgggg cgatcttgcc ctgctgccac aaacttagtg cggcggaaat
    39781 cagcgccggg ttctggcgtt tcaatgcttc tacaaacatc ttgttgcctc gtttctgttc
    39841 cttgaattaa atattcattt ttttgaatat ttaggttgtg cgacgtggat ttgtcgggag
    39901 cttcaaacct cttcgttagc agccatccat aatccataaa ggttggccag caaatagcct
    39961 tcttcacacg ggttaacttc cagtgcgaat tccttcaaca acacctgatg caattgcaca
    40021 acggcttgcc agtggctgga ttgcgccagc tctgccagca attcgttatc cagcggctct
    40081 atttcttcac cacggcgact gcgcatcagt gcactcgcca tatgtgtcat cgccatcgtt
    40141 ccttgctcac tgcgcaccgg cagatggcac tctgtttcca gtacgttgac gacctgcatc
    40201 atgcctttgc agatgtcctt atcaataacg cctgcctcgc aaagcaggtt gagtctggtt
    40261 tccatatcag atgcctttcg tttaataact cggttcctgt tccccactca ctacccgttg
    40321 ttgatgggcg agcagggcgt gtttatctac aacggcacgc agcagttgtt gcattgggta
    40381 atcatcgact cgcgtcagga ccacggcttc ggtggcctgt aaaaatcgcg gatcgtctgt
    40441 gagcggcgtc gcctctaatc ccagcatggt cagttcgttt tccgccacca cgttccagat
    40501 caccgcatcg acatcgcctt taacaatgcg ttgtaaactc tcgtgataag agagatcgac
    40561 acgttccaca tcactatcgc caaaaaagac atcggtcatg attttctgat ccgccgaacg
    40621 gttatccagc cccacgcgct tcacgtttgc ggactcgcct ttacggcaaa tcaactggtg
    40681 ctcgccaacg taggtgtgcg gccccaactc cagcgcgagg caaaaccctt tttgcgtgag
    40741 ataactttcc gccgccagtc gggaaaccac cgccatgtca tacacgccgt taagtaaaca
    40801 ctccacgcga atatccgcgc cgcgcatatg cgcatagtaa aaaggaatgc catcaaactg
    40861 ggctttcaat ccgctcgcta aaccttcgta caagcgggta tagggcaaag gcatcgcaca
    40921 taccacgttg ttgatatcca catgactcag caatgctttg ttatccatct cgaccagata
    40981 actgccattg cgcccacggc gttcaatccg tatcgctccg cttgattcca gcgttttcaa
    41041 cgcggcctgc gtcaggccaa cggatgaacg gcattcagtt gccagctcat ctatggtttt
    41101 caacctatta ccgcattttt cacccaacaa ataacgggcc agcgtcgtga taacgacgcc
    41161 ttcttttttg ataaacgttc gacgcataat atattttcaa taaaatgaat gtttatatct
    41221 tcattaaaat gaagataagt ggcaactgtg agatgtggaa acggcgaggc gcttcacatt
    41281 ttttcggatt attgcaaatg aggataaaaa aaaccgggtt tccccggttt cagagtgatg
    41341 acgaagtgat ttattcgaca atgcgagcaa agggtaattc ccactggcgc ttgcctgctc
    41401 ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccgagcgtt ttgatattac
    41461 ggcttcgcca caaaaccaat cgcttcgtac accgctttca gcgtacggga agcgtgtaca
    41521 ctggcttttt ctgcgccgtc tttcatcacc tgttgcagga aggcttcatc gttgcggaaa
    41581 cggtgatagc gttcctgcaa ttcagtcagc ataccggaaa cggcatcagc cacttcacct
    41641 ttcagatgac catacatctt gccttcgaac tgtttttcca gttctggaat gctctggccc
    41701 gttaccgcag aaaggatatc cagcaggttg gaaacgcccg ctttgttctg cacatcgtag
    41761 cgaactaccg gcggctcgtc ggagtcagtg accgcgcgtt tgattttctt cactaccgat
    41821 ttcggatctt ccagcaggcc gataacgtta ttgcggttat cgtcagactt ggacatcttc
    41881 ttggtcggct ccagcagcga cattacgcgc gcgccagatt tcggaataaa cggctccggc
    41941 accttaaaga tctcgccata cagcgcgttg aaacgctggg cgatatcgcg actcagttcc
    42001 aggtgctgtt tctggtcttc acccaccggt accagattag tttgatacag caggatgtcc
    42061 gcagccatca gcaccggata gtcaaacaga ccagcgttga tgttctcggc ataacgcgca
    42121 gatttatctt taaactgcgt catgcggctc agttcgccga agtaggtata gcagttcagt
    42181 gcccagccta actgcgcatg ttctggtacg tgggactgaa caaaaatggt gcttttctca
    42241 ggatcgatac cacaagccag atacaaggcc agcgtatcca acgtcgcttt acgcagcttc
    42301 tgtgcatcct ggcgcacggt gatcgcgtgt tggtcaacga tacagtaaat gcaatggtag
    42361 tcatcctgca tgtttaccca ctgacgcagc gcacccatgt agttaccaat ggtcaattca
    42421 cctgagggct gtgcgccact aaaaacgatg ggcttagtca tttttcgatt cctgattttc
    42481 gctatgcgga agccctaatg cgggcagaag gtcatttata gactgataaa ttacatcagg
    42541 ctggctgaga tcgatagcct cgccgtagtt atatccgtag gttaagccaa ctgatgggca
    42601 acctgccgct tttgccgcct gaatatcatt gcgtgagtcg ccgacaaaca gcatctgttg
    42661 tggggcaatt cccatccgct cagccaccag taacagcggg tccggatgcg gttttttgtt
    42721 ttgcacatca tcgccgccga taaccacgct aaagtatttg gcaatatcta aggcttcgag
    42781 cagcggcgcg acgaacggcg tcggtttgtt ggtgaccagg cctagcggca ggcctttagc
    42841 ctgcaacgcg cccaacgtat cggcaacgtg cgggaacaaa aacgtcccct cttcggcaac
    42901 ctcgccatag tagcgatcga acagtttacg cagaatacgt acctgttctt ctgccggaat
    42961 gtcgtcatca acgggcggtt tacccattgt tttacgctga gtcgcacgtt cctgacgcgc
    43021 ccaggtcaat gcgcgctcca tcagaacatc tgcgccgtta ccaatccagg taataacgcg
    43081 ttcttcacct gcgacgggca actccagcgc atacagcgcc atatctaccg cagcagcaag
    43141 accgggagca ctgtcgacca gcgtaccatc aagatcaaaa gcgacgccgc gaatatcttc
    43201 aaacttattc atgacttacc tttgccagtt cactgcgcat ttcatcaatg acttttttgt
    43261 agtctggctg gtcgaagatt gccgaaccgg cgacgaacat atccgcgccc gccgcagcga
    43321 tttcgccaat gttgttcacc ttcacgccac cgtctacttc cagacgaatg tcaaagccag
    43381 actcgtcgat acgacgacgt acttcgcgca gtttatccag tgtttgagga atgaacgact
    43441 gaccgccgaa accaggattg acggacatca gcaggatcac atccagctta tccatcacgt
    43501 aatccagata gctcagcggt gtcgccgggt taaataccag tcccgcttta cagccatttt
    43561 ctttaatcag ttgcagcgtg cggtcaacat gctcggaggc ttctggatga aaggtaatga
    43621 tgctggcacc agcggcagcg aaatcaggca caatgcgatc gacaggtttc accatcaggt
    43681 gtacgtcgat aggggcggta atgccgtagt tacgcaagga tttcagcacc attggcccaa
    43741 tcgtcagatt gggaacatag tggttatcca tgacgtcaaa atgcacgaca tcagcgccag
    43801 ctgccagggc ttttgcggta tcttcaccca ggcgggcaaa atcagccgac agaattgagg
    43861 gggcaatcaa atactgtttc atccgcttct ccttgagaat tattttttcg cgggtgaaac
    43921 gactcctggt ttgtacaaag ccagcagttc gtccaccttt ttacgtgtgc cgccgttgct
    43981 gcttatactg cgtcgaactt tgacgacatg caattttgcg cgctgatacc actcacgcgt
    44041 taacatcgta tcgtgattgg agatcagcac tggaatatgg cgctcaacca gaccttcggc
    44101 gatctccgcc agatgcgctt gttgttcaag cgtaaaactg tttgtgtgat acgccgtaaa
    44161 gttggcggtc gcagacagcg gtgcataagg cggatcgcaa tagacgacgg atgcatcatc
    44221 tgcgcgcgcc atgctatcgg cgtaagactc acaatagaaa aaggcattct gcgctttttc
    44281 agcgaagtga tacaactctg cttccgggaa atagggtttt ttgtagcggc caaacggcac
    44341 gttaaactca ccgcgcagat tgtaacgaca caggccgttg taaccgtagc ggttcaaata
    44401 taaaaacagt accgcccgac ggaacggatc ctggcttttg ttgaactctt cgcggaactg
    44461 atagtagacc tcggcgcaat ttgtttcggg aacaaacagc tcgcgtgcgg cctgtacgta
    44521 ctcatcagta cgcatcttca caatgttata gagactgatc aggtcgctat tgatatcggc
    44581 aaggatataa cgagaaaagt cggtgttgag aaacaccgac ccggcaccta caaaaggctc
    44641 aaccagacat tcgcccttgg gcaaatgccg tttaatatca tcaagcaggg gatacttgcc
    44701 ccctgcccac ttcaaaaaag cgcgattttt cttcatgctg actaactaat tacaccttct
    44761 ccggctgtgg agaaagctcc gacagcatcc agcgcttcag gcaactcccg caacgataac
    44821 ccattgcggg agataacctt gattacttca gatcggcctg tacctgacgc agcggtttcg
    44881 cccacgggtt tttggcctga acatctgctg gcaatgtaga taccgctttt ttcgcctctt
    44941 ctttcgaagc gtacacgcca gaaaccagga cataccacgg ctgaccatta cgcgtcgttt
    45001 catagacaac gtagtttttc agattctctt tcttcgccca accgttcagg ttgtcgtagt
    45061 tagaggaact gctcagctgc agagtgtaat ggctggacgg tgccgatttc aacgaaccaa
    45121 cattacctgc ggtcttccct ccagcggctg gtgttgccgt ggtttgcgcc ggggatgccg
    45181 tctgtactgg agccgtagtc gccgtctctt ttggtgctgg cgcagaagtc gcagccggtg
    45241 ccttcgtgct cgccacagga gcagctggtt cagtacgctt cggcgtctgt gctaccggct
    45301 tcggttccgt tttcacggtt gcttgcggtt ttttcggttc aatcaccgcc tgctgacgag
    45361 ctggacgcgt agtggccgga cgttcagcag tttgcgtttt cgccgtgtca cgcgatgcat
    45421 tgccattgcg tactggcgcg acagttgctg gttcagtcgg caatgtagaa ttgaccgcca
    45481 cattgttcaa ctgttgctga ttttgtggct gggtcagcgc attgttcagg tcaccctgca
    45541 cttcaacacg ttgttgacca tccgttgccg ccggggtttg cccttgagtc ggcgtagaag
    45601 agatcggtgg cagagaaaca tcctgctgag tattttccgc agacgtggtt cccggcgctg
    45661 gctgcacccc attcgcctga tcggtcgcat tgcctgcaag atcaatactc ttctcgccag
    45721 acgcggtttg atcgctggaa gaggtcgagg gggcttttag cgcagaaccg ataccgatga
    45781 tcaacagcag tagaaccaga atgccgacgc ccatcatcat atactgacga gaagcgggtt
    45841 tactggctgc ttttttgcgc ttacgcggac gacgctctac gcgctcttca tccacggttt
    45901 catcttcgga ttcgtcaatt tcttcttcga tttccggttc ctcattgcgc tctttttgcg
    45961 cacgagtcgg acggcgatcg tcagtgtcat caagttcaat atcatcaaaa ttgatctgcg
    46021 gttcgccacg ttcagtacgc tcagaacgtt cagaagattg acgagaacga ccagtacgac
    46081 gatcgctggg atcgggtttc agctcgtctt ctggtttgaa ttcatccatt taacacccca
    46141 ctaaaaggtt aatgcttacc acgttgcaat taacctgaag ctaatagacc gcttgataag
    46201 cggcctgacc tttcttgttg ttacgctgat tgacaatcgg caatggcgtt aagaacaagc
    46261 tcgtgcgaaa cgccgctgcg aacttcactc ttaccaattg ccaacggaag aattaagcgt
    46321 atctctcccg caaggacttt cttgtcacgc agcatatgcg gtaaatacgc ctgcgcggac
    46381 atttcgcgcg gcccattgac cggtaaccca gcccgcttga gcagggttat aatacgctgc
    46441 gtttcggcag aactaaactg cccgagacgt tccgacgtcc gcgccgccat caccataccc
    46501 gcagcgaccg cttcaccatg taaccaattg ccatacccca tttcagcttc aatggcatga
    46561 ccaaaggtgt gtcccagatt cagtaaagca cgtaacccgg tttcgcgctc gtcggcggcg
    46621 acaacttctg ccttcagttc acaacaacgg cgaatacagt acgccattgc cggaccgtcc
    46681 agacgcaaca acgcatccag attctcttcc agccagttaa aaaacgcacc gtcaagaata
    46741 atgccgtatt tgatgacttc tgccagcccc gacgctaact cacgcggggg aagcgttttc
    46801 agacagtcga gatccaccac cactgaagca ggctggtaga acgcgccaat catgttttta
    46861 ccgaggggat ggttgaccgc agttttgccg ccaacggagg aatcgacctg cgacagtaac
    46921 gtcgtcggga cttgaatgaa acgaacaccg cgctgataac tcgccgccgc gaagccggtc
    46981 agatcgccca ctacgccgcc gccaagcgcc accagcgtag tatcgcgacc atgcggcttt
    47041 tgtaacaacg ccgtaaagac ggtatcgagt acagccaggc ttttatactg ctcgccgtca
    47101 gggaggataa cgctatcgac gttaacaccc gcctgttcaa gtacgccgcg gaccttatcg
    47161 agatacagag gagccagggt ttcgttggtg accaacatga cctgctcgcc cgatttcagc
    47221 ggtaagaatg aagctggttc attaaacaaa ccagatgcga tggtaattgg gtaactacgt
    47281 tccccgagag taacgacaat cctctccata acgcgacatc caccttaatt actgtacccg
    47341 cagacgagtg tatataaagc cagaattagt tgctttccag catgtgaata atctggtttg
    47401 caaccacttt agcgctttga tcatcagtac gaatggtcac gtcggcaatc tcttcataca
    47461 gcggattgcg ttcattggcc aacgcttcca gaacttcacg cggcggtgtt tcaacgtgca
    47521 gcaacgggcg ttttttatca cgctgcgtgc gtgcaagttg cttttcgatg gtcgtttcaa
    47581 gataaacgac aacgccacga gcggaaagac ggttacgcgt ttcacgggat ttcacagagc
    47641 cgccgccagt agccagcaca ataccctgtt tctcggtcaa ctcattgatg accttttctt
    47701 cgcgatcgcg gaagccttct tcgccttcta aatcgaaaac ccagcccaca tcagctccgg
    47761 ttcgtttctc aatctcttga tcggaatcgt aaaattccat attgagttgt tgagctaact
    47821 ggcgcccaat agtgcttttt ccggcaccca taggcccaac cagaaagata ttgcgtttct
    47881 ctgccatttt ttcggtacta ctaagactat tcgttaatga taaacccgct tcgctcagag
    47941 agcgccgcag gacatgaact gaaacctcat aagatattgc gagagtcaga ctgaaaatta
    48001 tctcaatact ccagcgggtt tggcaactga ataaatcacc catccctctg catatctggt
    48061 cgctgcaagc gcgctgcctt gctaccaccg ctctggcgat aaatcaccgg gtaagattag
    48121 cgtaaaaaag acagcaaaat gccgcctgaa tgataaatca tcatcatgga acgcaggcag
    48181 cgaagagcac caactctcaa atcggtactc cttgtatgct aaatacctgc gcgcgtcaaa
    48241 tagatgaaac acgttcagca taaaaacatt accgctttta cggctgttta ctcactggaa
    48301 accagtcgtg gcgtgataaa caccactaac tcgcgtcgtt catcttcttt tccgtcatga
    48361 cgaaataatt gcccgaacca gggaatgtcg ccaagcaacg gtacgctatc ctgacccgat
    48421 ttatttttac gggtaaaaat gccgcccagc gccaacgttt ctccgctttt gacctcgacc
    48481 tgcgtttcga tctcctgctt atcaatcgcc agcacttcgc catcggcctg ctgtagcacc
    48541 tgccccggaa cgttctggct gatgtgtaat ttcagccgga tgcgaccttt ttgtaacacc
    48601 gtgggcgtga cctccatccc caggacggcc tctttaaatt ccaccgacgt cgcgccactt
    48661 tccccgctgg aaacctgata tggaatttcg ctcccctgtt taatgctggc aggctgaaga
    48721 tgtgaggcca gcagacgcgg actggcgata atatccagct gctgtttttg ttcgagcgca
    48781 gaaagctcaa gatccagcaa gcgtccgttg atgcgcccaa tgttaaaacc gacatgcgtt
    48841 gtcgccgtcg ctacggagag gtcgctacca agcgtggtga cttgcccaac gccaccagcg
    48901 tgttgcgcat cggccagcgt ccatttcacg cctaactcac gcaaactttt ttcattaatg
    48961 gtgacaatat gcgccgacag ctcaacctgc ccgaccggca gatccatttg cgctacccac
    49021 tgttcaagcg cgcttaacgc cgttttgtta tctcgtagca aaaggcgatt ggtgcgttta
    49081 tcgacggtca tactcccttt ggcactcagt agcttctccc ccgctttcgc cagttctccc
    49141 gcgtcggcgt attgcagggt tatactgcga ttttccagcg gcagatttgc ctgcgcccgc
    49201 gcctgctccg cctcctggcg ggcgatattg ttattctgcc aggcaatgga atgcactgag
    49261 agaatgttgt cttcctgccg cgttatcagt ccggcgcttt tcactacagt ttgtagtgcc
    49321 tgcttccagg gaacatctgt tagatgtaac gacaccgtac cgctgacgtc tggcgacacg
    49381 accaggttca acttctcctg ttcagccagc gcctgcaaca cctgagctac cggaacgtca
    49441 tccaccatca gcgtcacttt ttgcggcttt gccgcctgta cgccgggtat cagcatcaac
    49501 agtagtgcgg ctatccattg cttcatttgt atctccttgc cgttgccaca accattgtgg
    49561 cggttcgcag tttgtcccgg ttcccagcgt tagtgcgtct ggcgtcagct gtaaaattgt
    49621 ccagccgttt tccagcacat cgttttgctg cacccgtcgc catttctttt gcccgtcttt
    49681 tattacaccg atgatgtgct cgcctcgccc taccatcccc tgatagcgcc actggctaag
    49741 ttcgctaatc cggcatagat cttccggcgg tttaaaaggg tcacgcatac cggttaaaag
    49801 gcacaatgca atacctgcca acaaccagcg tttaaccctc atgcggtgtc tccagttgta
    49861 gcgtgaacaa aagatcatca ccttccacgc ttaacgaaaa acggctcacg ctgacgttgc
    49921 gctctgccag ccgtgtaaat gccgacggca ctgcttccca cagcgttttc aacgccaact
    49981 cacctccctg cgcggatgga tgccaggaaa ccagttgcgc gccgggcaac tgaaaatcca
    50041 gtggcgaaaa gggcagcgtt ttttcttcgc tgaagggagt ggtgtcaacc aggcgataca
    50101 gtgtggccca ctgctggtga tgacttgccc gtagtcgaat taatgcgtcg cgctcttcat
    50161 ggtgtgtcga tgacagaaaa atgagcgtaa ctaacatcag caaccagaat gcccagcaaa
    50221 actggcggag gcggggtgat gtggcgaacc accagtcaaa gaacatgttc atcgctaacc
    50281 ttccttgtta actgatactc aaattgccag cgtccctgcg catcctgctg cgtggctccc
    50341 cgctgattga gatgaaaaga cgcatcctgg cggagtgacg tttctagtgc gtttaacgcg
    50401 gtaatgcttg ttgtcagccc cttgatctcc agcgttccct gctgccagct tatcgttgtc
    50461 agccaggcgt gctcgggtaa aagcgccgcc agtgcttcca gcgcagattg ccagtcgcgg
    50521 gtaaattgtc gctggcgctg ccgttgcgaa cgctgttcgc gtaactgctg ttgctccagc
    50581 aaacgtggct tcgttatctg taagctgcgg gcgagttgtt gttccgcctg aagcaaaact
    50641 gcgtctatgc gcgcttcggc gctgcctgtc agacgcagta ttagcgttat cccgacggcc
    50701 agcagcagag gcgcaacgaa catcagcaac cagaaacgca gaaaagcggt ccggcgttgc
    50761 tgtcgccagg gcaaaaaatt aattggcggg ttcatcagta cgccttccca agcgccagcc
    50821 ccagcgcgat ggcaaagtct ccaccaggcg gcggtagcgg cggctgacga acagaaacgg
    50881 cctcccaggg gtcaaatccg ccttcgccac atatcgcgac gctgtcggga tcaacggata
    50941 acgctgccgc cagctccttc gcgctagtca tccccaccgc cagtttgcgc ccccagctat
    51001 agcgcgtcgc ccacagccac tgttcgttat cacgccaggc cagacattgc tgatgagaag
    51061 gtaaaaaagg caggaatcgc tgtaatgcac tggcatccgg ggtgatcgca ctaacatgaa
    51121 cacgcaacct ttctgccaga gtaagcagcg ttgccagctc tttgctttgc gcggcagtca
    51181 cgttataggc gggactgagt gagtcttcgc tgtaatcgaa acgcagggag tccggatcca
    51241 tatccagctc gcgggccatc gtccctgaca gccaggccgt ttgctcccgc tcaccaaggg
    51301 acatcgacgg gcgcggaaat gaccgctgta atgtgcgact ggcgggaaac gccaacataa
    51361 tgtgatgacg ctgcggcagt tcgcgactcc acggtaacaa cgttttagcc agctgctgcg
    51421 catcaacaat ccgcccatct ttgataatgt cgttctccag cggcaaccgc caccagcgtt
    51481 gcaaaaagca ttcttttgct ccccgcacga tcgcaaccgc taccgcttct tgctgttgta
    51541 aatgcaaacc aatttgccag atcttaaatg ccattgtgat gatctcctta tcacccgtca
    51601 ctctgacggg tatatcaatg cgtctggctt gcctttatac taccgcgcgt ttgtttataa
    51661 actgcccaaa tgaaactaaa tgggaaattt ccagtgaagt tcgtaaagta ttttttgatc
    51721 cttgcagtct gttgcattct gctgggagca ggctcgattt atggcctata ccgctacatc
    51781 gagccacaac tgccggatgt ggcgacatta aaagatgttc gcctgcaaat tccgatgcag
    51841 atttacagcg ccgatggcga gctgattgct caatacggtg agaaacgtcg tattccggtt
    51901 acgttggatc aaatcccacc ggagatggtg aaagccttta tcgcgacgga agacagccgc
    51961 ttctacgagc atcacggcgt tgacccggtg gggatcttcc gtgcggcaag tgtggcgctg
    52021 ttctccggcc acgcgtcaca aggggcgagt accattaccc agcagctggc gagaaatttc
    52081 ttcctcagtc cagaacgcac gctgatgcgt aagattaagg aagtcttcct cgcgattcgc
    52141 attgaacagt tgctgacgaa agacgagatc ctcgagcttt atctgaacaa gatttacctc
    52201 ggttaccgcg cctatggtgt cggtgctgcg gcacaagtct atttcggtaa aacggtcgac
    52261 caactgacgc tgaacgaaat ggctgtgatc gccgggctgc cgaaagcgcc ttccaccttt
    52321 aatccgctct actcaatgga tcgtgccgtc gcgcggcgta acgtcgtact gtcgcggatg
    52381 ctggatgaag ggtatatcac ccaacaacag ttcgatcaga cacgcactga ggcgattaac
    52441 gctaactatc acgcgccgga gattgctttc tctgcgccgt acctgagcga aatggtgcgc
    52501 caggagatgt ataaccgtta tggcgaaagt gcctatgaag acggttatcg catttacacc
    52561 accatcaccc gcaaagtgca gcaggccgca cagcaggcgg tacgtaataa cgtgctggac
    52621 tacgacatgc gccacggcta tcgcggcccg gcaaatgtgc tgtggaaagt gggcgagtcg
    52681 gcgtgggata acaacaagat taccgatacg ctgaaggcgc tgccaaccta tggtccgctg
    52741 ctgcctgccg cagtcaccag cgccaatcct cagcaagcga cggcgatgct ggcggacggg
    52801 tcgaccgtcg cattgagtat ggaaggcgtt cgctgggcgc gtccttaccg ttcggatact
    52861 cagcaaggac cgacgccgcg taaagtgacc gatgttctgc aaacgggtca gcaaatctgg
    52921 gttcgtcagg ttggcgatgc atggtggctg gcacaagtgc cggaagtgaa ctcggcgctg
    52981 gtgtcgatca atccgcaaaa cggtgccgtt atggcgctgg tcggtggctt tgatttcaat
    53041 cagagcaagt ttaaccgcgc cacccaggca ctgcgtcagg tgggttccaa catcaaaccg
    53101 ttcctctaca ccgcggcgat ggataaaggt ctgacgctgg caagtatgtt gaacgatgtg
    53161 ccaatttctc gctgggatgc aggtgccggt tctgactggc agccgaagaa ctcaccaccg
    53221 cagtatgctg gtccaattcg cttacgtcag gggctgggtc agtcgaaaaa cgtggtgatg
    53281 gtacgcgcaa tgcgggcgat gggcgtcgac tacgctgcag aatatctgca acgcttcggc
    53341 ttcccggcgc aaaacattgt ccacaccgaa tcgctggcgc tgggttcagc gtccttcacc
    53401 ccaatgcagg tggcgcgcgg ctacgcggtc atggcgaacg gtggcttcct ggtggacccg
    53461 tggtttatca gcaaaattga aaacgatcag ggcggcgtga ttttcgaagc gaaaccgaaa
    53521 gtagcctgcc cggaatgcga tattccggtg atttacggtg atacgcagaa atcgaacgtg
    53581 ctggaaaata acgatgttga agatgtcgct atctcccgcg agcagcagaa tgtttctgta
    53641 ccaatgccgc agctggagca ggcaaatcag gcgttagtgg cgaagactgg cgcgcaggag
    53701 tacgcaccgc acgtcatcaa cactccgctg gcattcctga ttaagagtgc tttgaacacc
    53761 aatatctttg gtgaaccagg ctggcagggt accggctggc gtgcaggtcg tgatttgcag
    53821 cgtcgcgata tcggcgggaa aaccggtacc actaacagtt cgaaagatgc gtggttctcg
    53881 ggttacggtc cgggcgttgt gacctcggtc tggattggct ttgatgatca ccgtcgtaat
    53941 ctcggtcata caacggcttc cggagcgatt aaagatcaga tctcaggtta cgaaggcggt
    54001 gccaagagtg cccagcctgc atgggacgct tatatgaaag ccgttcttga aggtgtgccg
    54061 gagcagccgc tgacgccgcc tccgggtatt gtgacggtga atatcgatcg cagcaccggg
    54121 cagttagcta atggtggcaa cagccgcgaa gagtatttca tcgaaggtac gcagccgaca
    54181 caacaggcgg tgcacgaggt gggaacgacc attatcgata atggtgaggc acaggaattg
    54241 ttctgattaa aaaggcgctt cggcgccttt tcagttggct gacaaagtgc atttgtttat
    54301 gccggatgcg ggtgaacgcg ttatccggct aacaaaatcg tgaaaaatca atatattgca
    54361 ggaaacccat aagcctgata agcgttgtgc atcaggcaaa cttcacgcat ttacactcgc
    54421 ccctgccctt tcaaccattc gcgcacgagg aacagcgcgc tgacattacg cgcttcgttg
    54481 aagtcagggt cttccagcaa atccatcata tgcgccagcg gccagcgcac ctgtggtagc
    54541 ggctctggct catcgccttc cagcgattcc gggtagagat cttgcgcgac cacgatattc
    54601 attttgctgg aaaagtaaga cggtgccatg ctgagcttct tcaaaaaagt cagatcgttc
    54661 gcaccaaatc caacctcttc ttttagctcg cggttagcgg cttcgtagac gctttcaccc
    54721 ggatcaatta atcctttcga aaaacctaat tcgtaggatt cagttcccac tgcgtattcg
    54781 cggatcagga tcaggtgatc gtccacaatc ggcacaatca tcactgcttc ccggttggtt
    54841 ggacgcattc gttcataaac acgccgcacg ccattgctga actccagatc cacgctctcg
    54901 acggtaaaca gtcgggaacg ggctacagtt tcaacattca gaatggtggg tttttgtaat
    54961 gatttgctca tcgtgggatc tatgctgtga aatcagcggt tattgtgcga tatcggacac
    55021 gctttcggca atgtgaattg catgttattt acatttatgt aacttaataa ataatcgtcc
    55081 tcaaatcaaa ttaaaagtca ataggttgaa ataactccag gaatttgctg atattccgcc
    55141 ttcagagggt ttgctatgat cagcggttac tgtgatgtgc ttaatgatgc tcaagttaaa
    55201 ctccacgctt gccgatagcc aaccgcagaa tcatgtattg tgtctggtgc gactgaccac
    55261 gcctgacaga ctaagtaaga tggggaaagc atgagcacca ttgtgatttt tttagctgct
    55321 ttgctggcct gctcactact tgcgggatgg ctgataaaag tgcgatccag acggcgtcag
    55381 ctgccctgga ccaacgcctt cgcggatgcg caaacgcgta aactcacacc tgaagaacgt
    55441 agcgccgttg aaaattatct tgagagcctg acgcaggtat tacaggtgcc tggcccaacg
    55501 ggagccagcg cggcaccgat ctctctggcg ctgaatgccg aaagcaacaa cgtcatgatg
    55561 ctgacacacg ctatcacgcg ttacggcatc tctaccgacg atccgaataa atggcgttac
    55621 tacctcgatt cggtagaagt ccacctgccc cctttctggg aacaatacat caacgatgag
    55681 aataccgttg aactgattta taccgattcg ctgccgctgg ttatttcact caacggtcat
    55741 acgctgcagg agtacatgca ggaaacgcgc ggctatgcct tgcaacctgt tccgtccacg
    55801 caggcgtcga ttcgcgggga agagagtgag caaatcgagc tactcaatat tcgcaaagaa
    55861 acgcatgaag aatatgcgct gagtcgtccg cgcgggctgc gtgaagcgtt gctgatcgtc
    55921 gcctccttcc tgatgttctt tttctgcctg attaccccgg atgtatttgt tccgtggctg
    55981 gcaggcggcg cattactgct gctgggcgca ggtctgtggg ggctattcgc gcccccggca
    56041 aaatcctccc tgcgagaaat tcattgtctg cgcggtacac cccgtcgttg gggattgttt
    56101 ggcgaaaacg atcaggaaca gatcaacaat atttcactcg gtattatcga cctggtctat
    56161 cccgcacact ggcagccata cattgctcag gatctcggtc aacaaaccga tattgatatc
    56221 tatctcgacc gccacgtagt gcgtcagggg cgatatcttt cgctgcatga tgaagtaaaa
    56281 aactttccgt tacagcactg gctgcgcagt acgattatcg ctgcgggctc gctgctggtg
    56341 ctgtttatgc tgttattctg gatcccgctg gatatgccgc tgaaattcac tctctcatgg
    56401 atgaaaggcg cgcagaccat tgaagccacc agcgtaaaac aactggctga cgctggcgtg
    56461 cgggtaggcg atacattgcg tattagcggt acgggaatgt gtaatattcg cacttccgga
    56521 acctggagcg cgaaaaccaa ttcacctttt ttaccgtttg actgctcgca gatcatctgg
    56581 aatgacgccc gctcattacc attaccagaa tctgaactgg tcaacaaagc gacggcattg
    56641 actgaagcag ttaatcgcca gctgcaccct aaaccggaag atgaatctcg cgtcagtgcc
    56701 tcattacgtt cagcgattca aaaatccggc atggtattgc ttgatgattt tggcgacatt
    56761 gtactgaaga cagcggattt atgttctgcc aaagatgact gtgtgcgact gaaaaatgcg
    56821 ctggtcaatc tcggcaacag taaagactgg gacgcgctgg taaaacgcgc caacgccggg
    56881 aagctcgatg gcgtgaatgt gttattacgc ccggtgagtg cagaatcgct ggataacctg
    56941 gtggcaacct ccaccgcgcc gtttatcacg catgaaacgg cgcgagcggc acaatcacta
    57001 aacagtccgg cccccggcgg attcctgatt gtcagcgacg aaggcagcga ttttgttgat
    57061 cagccctggc cttcggcatc actttacgac tacccgccgc aagaacagtg gaacgctttc
    57121 cagaaactgg cacaaatgct gatgcatacg ccgtttaacg ccgaaggtat cgtcacaaaa
    57181 atcttcactg acgccaatgg tacgcagcat attggccttc atccgatccc ggatcgttcc
    57241 ggcctgtggc gctatctcag caccacattg ctgctactga cgatgctggg tagcgccatt
    57301 tacaatggcg tacaggcctg gcgtcgttac cagcgtcatc gcactcgcat gatggagatt
    57361 caggcctatt atgaaagctg cctgaacccg caactgatca ccccttcaga aagccttatc
    57421 gaataacacg tttgcgcggc aggttatgct accctgtcgc gcaaattgct tcactctgga
    57481 gatttccctc atgcatatca acattgcctg gcaggacgta gataccgttc tgctggatat
    57541 ggacggcacg ttgctcgacc tcgccttcga taactatttc tggcaaaagc tggtgcctga
    57601 aacatggggc gccaaaaacg gggttacgcc acaggaagcg atggaatata tgcgccagca
    57661 atatcacgac gtacagcata cgctaaactg gtactgtctt gattactgga gtgagcaact
    57721 gggtctggat atctgtgcga tgaccaccga gatgggaccg cgtgccgtac tgcgtgaaga
    57781 taccattccg tttcttgagg cactgaaagc cagcggtaag cagcgaattt tgctcaccaa
    57841 tgcgcatccg cacaacctgg cggtaaaact tgagcatacc ggtctggacg cacaccttga
    57901 tttattactt tccacccaca catttggtta tccgaaagag gatcagcggt tatggcatgc
    57961 ggtggccgaa gctacgggtc tgaaagctga aagaacgctg tttattgatg acagcgaagc
    58021 gattctcgat gctgccgcgc aatttggtat tcgttactgc ctcggcgtga ctaatcctga
    58081 ttccgggatt gccgagaaac agtatcaacg ccatccgtca ctgaatgact accgccgcct
    58141 gatcccctcg ctaatgtgaa ggagacgcca tgaaagagaa acctgctgtt gaggttcgac
    58201 tggataaatg gctatgggct gcccgttttt ataaaacccg cgcgctggcc cgtgaaatga
    58261 ttgaaggcgg taaggtgcat tacaacgggc agcgcagcaa gccgagcaaa atcgtcgagc
    58321 tgaatgccac gctcactctg cgccagggaa atgacgaacg cacggtgatt gtaaaggcga
    58381 ttactgaaca gcgtcgcccc gccagcgagg cagccttgct gtatgaagag actgcggaaa
    58441 gtgtagagaa acgcgaaaaa atggcgatgg cacgtaaact taatgcctta accatgccgc
    58501 acccggaccg acgcccggac aaaaaagagc gccgcgacct gttacgattt aaacacggcg
    58561 acagtgaata actgtcacct gcaagagaga tgattatgcc gcaacatgac caattacatc
    58621 gctatctgtt tgaaaatttt gccgttcgcg gcgaactggt aaccgtttcg gaaaccctgc
    58681 aacagatcct tgagaaccac gattatccgc agcccgttaa aaacgtgctg gcagaactgc
    58741 tggttgcgac cagcctgtta accgctacgc tgaagtttga tggtgatatc accgtacagc
    58801 tgcaaggcga cggtccgatg aatctggcgg ttattaacgg taacaataac cagcaaatgc
    58861 gcggtgtggc gcgcgtgcag ggcgaaattc cagaaaatgc cgacctgaaa acgctggtcg
    58921 gcaatggtta cgtggtgatc accattaccc cgagcgaagg cgaacgctat cagggcgtgg
    58981 ttggtctgga aggtgatacc ctggcggcct gcctggaaga ttactttatg cgttctgaac
    59041 agctgccgac gcgcctgttt attcgcaccg gcgacgtaga cggcaaaccg gctgcaggcg
    59101 gtatgttgtt gcaggtaatg cctgcgcaaa atgcccagca ggacgacttt gaccacctgg
    59161 cgacgctaac cgaaaccatc aaaaccgaag aactgctgac cttaccggca aacgaagtgt
    59221 tgtggcgttt gtatcacgaa gaagaggtga cggtttacga tccgcaggat gtggagttca
    59281 aatgcacctg ctcgcgtgaa cgttgcgccg atgcgctgaa aacgctgcct gatgaagaag
    59341 ttgatagcat cctgacggaa gatggcgaaa ttgacatgca ttgtgattac tgcggtaacc
    59401 actatctgtt caatgcgatg gatattgctg aaatccgcaa caacgcgtct ccggcagatc
    59461 cgcaagttca ttaatgagta tgtccggcag agagggctct gccggatttt acgacgtaat
    59521 ggcctgatgt gctgcgctta ccaggcctgt gaaataaatc gcaaattatt gttggtcaga
    59581 tacagcgtcg tatccgttaa aagagttgtc acttacttaa gtttatttcc agcgtttcac
    59641 catctaccgt cagatcgcgc cacgctttcg gcctcgtccc cagttttcca tccttcgcag
    59701 ctgtgagtaa tttttctttc gttatttcgt tcggaagtaa actcatatca agcgcgtccc
    59761 attcttttct gtctgagtca aagccataga caatgactct gtcatcatta aaagcaaaca
    59821 ggatccgctc cgcctgacca tcactattca gatcttgctc gaccaggacg caggcgtctt
    59881 tttcaataca ggtcatcacg ttatagcgat cctggattaa ggccgaccag aatgtcgcat
    59941 ccggtttaac agaaccaggg gcaattaaca cattttcggc taatactttt tccgatacct
    60001 gttcctgaag atgttgctct ccatctaacg ccatcagcag atcccgcgcg cgtttcgggt
    60061 ctttcataaa cccggcatcg cttttcagcg actcaagcgc cgcacgtcca tagcgaccgc
    60121 tctgttcgag catgtagata gttacctggt ctggcgtgtt tttgccgctc tgataacgcg
    60181 ccatatgact gttcacgcta atacgcatac tatccagcac cggcgaatta agaagcacca
    60241 gtatcaccaa caccaataac gaaaccgcaa ggttcacttt gccctgaaga acaaggggat
    60301 tttgcccatt acgccagacg atactgacaa aataccccag cgaccacacc agtaacacca
    60361 gcaccgccag cgcgccctgc aagcggtcga cagtccagcc gtactgagcg acccgcagcc
    60421 ataacgccca ggcggcgacg aacacataca gcggagcaac cagcaaagcg gttttaatca
    60481 ggcaacgcaa cggccccgtc cagggaagtg acgctttttg cggatcgcgg acaatagcca
    60541 ttaagatcaa ttgcaaaaag gccagcgtta acagcaaccc ggcggcggag atgtggcgag
    60601 aaatcgcgct caggcccgta aacggcagag tgatgataaa catcagggtt agcaatgata
    60661 ctaacggcag caaccccgtg gcgattaacg tgaacaactt ttgaatagag tcgattaaac
    60721 gtgactgtgt gcgcgcgaga atcaccgcca gtgcggtaac cagacctaat gtaagataca
    60781 taaaccagtc ggttgcaaaa aagagcgtat tgaaaaactt gatgccaaca agtttaaaca
    60841 actcacccca cagtaaaagc accacccacg tcaggccatt ggcgagaaaa atcaccagta
    60901 atataagtac gttatgccat actgacttgt aaaagtagct atagcggcta ctgccattgc
    60961 ggatacgaag gctttgttgt atccacggta gcaacaacat tgccatcaac agcagatagc
    61021 agccaaaatc ccaaatcgcc ttttcagctc tccaggggtt catgccatca gtttgccact
    61081 tcagccaacc gctcatccct gtcgtggcaa taaacaccag cgccagccat ccccaaaggc
    61141 gcttctgttt aaaagagatc acggagaaga gtaaaacgga tgaaaacgca acggtcgcag
    61201 gcacgccata aacaatccaa ctgtgatttt ttccggcaag ccaggcgatc aacaggtagc
    61261 aaaccagccc ctgaagtaat ccggtagcaa tcatccccca gcgtgttgcc ggtgaaagtt
    61321 caacgttatc catgttggtt atccagaatc aaaaggtggg ttaattatcg catccgggca
    61381 gtagtatttt gcttttttca gaaaataatc aaaaaaagtt agcgtggtga atcgatactt
    61441 taccgattga atttgcatca atttcattaa ggaatgcgat tccactcaca atattcccgc
    61501 catataaacc aagatttaac cttttgagaa cattttccac acctaaaatg ctatttctgc
    61561 gataatagca accgtttcgt gacaggaatc acggagtttt tgtcaaatat gaatttctcc
    61621 agatacgtaa atctatgagc cttgtcgcgg ttaacacccc caaaaagact ttactattca
    61681 ggcaatacat attggctaag gagcagtgaa atgcgcgtta acaatggttt gaccccgcaa
    61741 gaactcgagg cttatggtat cagtgacgta catgatatcg tttacaaccc aagctacgac
    61801 ctgctgtatc aggaagagct cgatccgagc ctgacaggtt atgagcgcgg ggtgttaact
    61861 aatctgggtg ccgttgccgt cgataccggg atcttcaccg gtcgttcacc aaaagataag
    61921 tatatcgtcc gtgacgatac cactcgcgat actttctggt gggcagacaa aggcaaaggt
    61981 aagaacgaca acaaacctct ctctccggaa acctggcagc atctgaaagg cctggtgacc
    62041 aggcagcttt ccggcaaacg tctgttcgtt gtcgacgctt tctgtggtgc gaacccggat
    62101 actcgtcttt ccgtccgttt catcaccgaa gtggcctggc aggcgcattt tgtcaaaaac
    62161 atgtttattc gcccgagcga tgaagagctg gcaggtttca aaccagactt tatcgttatg
    62221 aacggcgcga agtgcactaa cccgcagtgg aaagaacagg gtctgaactc cgaaaacttc
    62281 gtggcgttta acctgaccga gcgcatgcag ctgattggcg gcacctggta cggcggcgaa
    62341 atgaagaaag ggatgttctc gatgatgaac tacctgctgc cgctgaaagg tatcgcttct
    62401 atgcactgct ccgccaacgt tggtgagaaa ggcgatgttg cggtgttctt cggcctttcc
    62461 ggcaccggta aaaccaccct ttccaccgac ccgaaacgtc gcctgattgg tgatgacgag
    62521 cacggctggg acgatgacgg cgtgtttaac ttcgaaggcg gctgctacgc aaaaactatc
    62581 aagctgtcga aagaagcgga acctgaaatc tacaacgcta tccgtcgcga tgcgctgctg
    62641 gaaaacgtca ccgtgcgtga agatggcact atcgactttg atgatggttc aaaaaccgag
    62701 aacacccgcg tttcttatcc gatctatcac atcgataaca ttgttaagcc ggtttccaaa
    62761 gcgggccacg cgactaaggt tatcttcctg actgctgatg ctttcggcgt gttgccgccg
    62821 gtttctcgcc tgactgccga tcaaacccag tatcacttcc tctctggctt caccgccaaa
    62881 ctggccggta ctgagcgtgg catcaccgaa ccgacgccaa ccttctccgc ttgcttcggc
    62941 gcggcgttcc tgtcgctgca cccgactcag tacgcagaag tgctggtgaa acgtatgcag
    63001 gcggcgggcg cgcaggctta tctggttaac actggctgga acggcactgg caaacgtatc
    63061 tcgattaaag atacccgcgc cattatcgac gccatcctca acggttcgct ggataatgca
    63121 gaaaccttca ctctgccgat gtttaacctg gcgatcccaa ccgaactgcc gggcgtagac
    63181 acgaagattc tcgatccgcg taacacctac gcttctccgg aacagtggca ggaaaaagcc
    63241 gaaaccctgg cgaaactgtt tatcgacaac ttcgataaat acaccgacac ccctgcgggt
    63301 gccgcgctgg tagcggctgg tccgaaactg taatgatttg aagctggaga atatctatcc
    63361 agtatcttat agaaagcaaa acgggaggca ccttcgcctc ccgtttattt acccttcttt
    63421 tgtcgtgccc tgcgcccgcg ttaccggcac tggcagccag gcgcgaatgg aaagcccgcc
    63481 ccgctcgctg gtgccaagct ccagcatccc gttatggtta tccacgatac gctgcacaat
    63541 tgccagccct aatcccgtgc cgctaatggt gcgcgcactg tcgccgcgga caaacggctg
    63601 gaacaggtgc ttacgttgtt ccggcgcaat tcccggaccg tcatcttcca cctggaacca
    63661 ggcgcgattc ggctccgttc cgctgctgac tttgatccag ccattgccat aacgggcggc
    63721 gttgaccacc atattcgcca ccgcgcgttt gatcgacagc gggtgcattt tcacttcaat
    63781 gctgccgggg taaagcgcgg tttcaatttc ccgctcatag ccactttcgg cagcaatcac
    63841 ctcaccgagt actgcattaa gatccgccat ttccatcggc atctcctgcc cggtgcgcag
    63901 gtagtcgata aactgctcaa tgatggcgtt gcactcttcg atatctttat tgatcgattc
    63961 tgccagatag ccatcctgct cgctcatcat ctcagtcgcc aggcgaatac gcgtcagcgg
    64021 cgtgcgcaag tcgtgactta cccccgccat cagcagcgtg cggtcatccg ccagttgctt
    64081 aacaccagcc gccatatggt taaaggcacg ggtaacggaa cgcacctcgg acgcgccata
    64141 ctcacgcagc ggcggcggaa taatcccttt accaacctgc aaggctgcgt gttcgagatc
    64201 gaccaacggt cggttctgga tacgaataaa cagccacgcc ccgcctatcg ccaatagcat
    64261 aatcgccagc gtatagcgga acagcggaga gaaatcgccc tgatgaattt cggtcagcgg
    64321 cacgcgtacc cagatattgg gcgacagcca ggttttcagc cagacgacag gcgaactttt
    64381 gttgacctca acgcgcactt ccgtcgggcc gcccagttgc tgtgccatct gatggcttaa
    64441 gaattcatag tgttgcgccc aacgcagacc tgcctcttcg gcagcctcgt tggagtagag
    64501 agagatcccc agctcacggt agatctcccg acggaaagcg ggaggcacaa ccaactgcgt
    64561 gccgtcctcc agttgcagtt tgtcggtcat caacatacgg acttcgtacg cgaggacttt
    64621 attaaactgc tggaggctcg gcaaaatcgc gaagttcagc accaccagat aagtcgtcac
    64681 caggctggcg aacagcaagg tgacgatgag caataacgta cgggcaaatg aacttcgtgg
    64741 cgagaagcgc aatcgcctca tgctttagag ccgtccggta caaagacgta gcccaggccc
    64801 caaacggtct gaatgtaacg cggatgcgct ggatcttctt ctaccatgcg gcgcagacgc
    64861 gaaatctgca cgtcgatgga gcgttccatt gcggaatatt cacgaccacg ggcaaggttc
    64921 atcagcttat cgcgggagag cggctcacgc ggatggctga ccagtgcctt cagtaccgca
    64981 aactcaccgc tggtgagcgg catcggctca tcttcgcgga acatttcgcg cgtaccgagg
    65041 ttaagtttga acttaccgaa agcaattacc gcctcttcct gtgacggtgc gcctggcagt
    65101 tcgttcgcct gacgacgcag caccgcacga atacgggcca gcagttcacg cgggttaaac
    65161 ggttttggaa tgtagtcgtc agcgccaatc tccaggccta cgatacggtc cacttcttcc
    65221 cctttcgccg tcaccataat gatcggcatc gggttgctct gactacgaag acgtcggcaa
    65281 atcgacaagc catcttcacc aggtaacatt aaatccagta ccataagatg gaaagattca
    65341 cgagtcagca ggcgatccat ctgttctgca ttagcgacgc ttcgaacctg gaagccttgt
    65401 tcggtgagat aacgttccag cagcgcacgc aggcgcatgt cgtcatcgac caccagaatc
    65461 ttgtagttct cttgcattgt ttgtactccc aaaggttcgc aacaatttgt aagcgtgtat
    65521 tcttaaaaaa gctcacgttc gtcaccagct aaatctggta tgaatttcag cctaaattgt
    65581 tacaaagcat attaaacagc agcttaagta tacaatttat tcagcgaaac attattgatt
    65641 ctgttgatat gatcacgtta tacccaatgt gcgcattatc aaacagacaa agggaatcaa
    65701 cgagatgaaa acgcccctgg ttacccggga agggtatgaa aaactcaaac aagagcttaa
    65761 ttatctctgg cgtgaagaac gcccggaggt cacaaaaaag gtgacctggg ccgcaagtct
    65821 gggcgaccgc agcgaaaatg ctgactatca gtataataaa aagcgtctgc gtgaaatcga
    65881 ccgtcgcgtg cgctatctca ctaaatgcat ggaaaatctc aaaatcgtcg attactcccc
    65941 tcagcaggaa ggcaaggtct tttttggcgc atgggtggag attgaaaacg acgatggcgt
    66001 gactcaccgt ttccgtattg tcggctacga tgaaattttt ggccgtaaag attacatctc
    66061 tatcgattcc ccgatggccc gcgcattgct gaaaaaagaa gtcggcgatc tggcggtggt
    66121 gaatacccct gccggggaag cgagctggta tgtcaatgct atcgagtacg tgaaaccgta
    66181 aggaagagtc ttaacctcct gccgatggct ggcatttttg ccagccagtc cgtataacta
    66241 tcccctgatt tttgatccga aaagatgaac tcaaaccatg atgaatgatt cgttctgccg
    66301 cattattgcg ggtgaaattc aggcgcgccc ggaacaggtt gacgctgccg ttcgcctgct
    66361 tgacgaaggg aataccgtgc cgtttatcgc acgttatcgt aaggaaatca ccggcggtct
    66421 ggatgacacg cagctgcgta atctggaaac gcgtctgagc tatctgcgcg agctggaaga
    66481 gagacgtcag gcgatcctca aatccatttc cgagcaaggc aaactcaccg atgatctggc
    66541 gaaggccatc aacgccacac taagcaaaac cgaactcgaa gatctctacc tgccctacaa
    66601 acctaaacgc cgcacccgcg ggcaaatcgc cattgaagca gggcttgagc cgttggctga
    66661 cctgctgtgg agcgatccgt cacacacgcc agaagtcgcc gctgcacaat atgttgatgc
    66721 agataaaggc gtggcagata ccaaagccgc gctggacggc gcgcgctata tcctgatgga
    66781 acggtttgcc gaagatgccg cgctgctggc gaaagtgcgt gattatctgt ggaagaacgc
    66841 gcatctggtc tcaaccgtga tgagcggtaa agaagaggaa ggggcaaaat tccgcgacta
    66901 tttcgatcat cacgaaccgc tgtccacggt gccttctcac cgcgcgctgg cgatgttccg
    66961 tgggcgtaac gaaggcgtac tccagctttc gctgaatgcc gatccgcagt tcgaagagcc
    67021 gccaaaagag agctattgcg agcaaatcat catggatcat ctcggcctgc gcctgaacaa
    67081 tgctccggcg gatagctggc gcaaaggcgt ggtgagctgg acctggcgca tcaaggtgct
    67141 gatgcatctg gaaaccgaac tgatgggcac cgtgcgcgaa cgcgcggaag atgaagcaat
    67201 caacgtcttt gcccgtaacc tgcacgatct gctgatggcg gcccctgccg gactgcgtgc
    67261 cacgatgggc ctcgatccgg gtctgcgtac cggggtaaaa gtagcggtgg tcgatgccac
    67321 tggcaaactg gtggcgaccg ataccattta cccgcacacc ggacaaacag caaaagcagc
    67381 gatgaccgtt gctgccttgt gtgaaaagca taacgttgaa ctggtggcga tcggcaacgg
    67441 tacagcttcc cgcgaaaccg aacgtttcta tctcgacgtg cagaagcagt tcccgaaagt
    67501 gaccgcgcag aaagtgatcg tcagcgaagc tggcgcgtcg gtttactcgg cttccgagct
    67561 ggcagcgcag gagttcccgg atctcgacgt ttcgctgcgt ggtgcggtat ctatcgcccg
    67621 ccgtttgcag gatccgctgg cggagctggt gaaaatcgat ccgaaatcta tcggcgtagg
    67681 tcagtatcag catgacgtca gccagacgca actggcccgc aaactggatg cagtagtaga
    67741 agactgcgta aacgccgttg gcgtcgatct caacaccgct tctgttccgc tattaacccg
    67801 cgtggcgggc ctgacgcgca tgatggcgca aaacatcgtg gcctggcgcg atgagaacgg
    67861 ccagttcaag aaccgtcagc aactgctgaa agtcagccgt ctggggccga aagccttcga
    67921 gcagtgcgcg ggcttcctgc gcattaacca cggtgataac ccgctggacg cctctaccgt
    67981 tcacccggaa gcctatccgg tggtggaacg cattctggca gcaacacagc aggcactgaa
    68041 agatctgatg ggtaacagca gcgaactgcg taacctgaaa gcgtctgact ttactgatga
    68101 aaaattcggt gtgccgacag taactgacat catcaaagag ctggaaaaac cgggtcgcga
    68161 tccgcgtccg gaatttaaaa ccgctcagtt tgccgatggc gtcgagacaa tgaacgacct
    68221 gcaaccgggt atgatcctcg aaggcgctgt caccaacgtc accaactttg gcgcgtttgt
    68281 cgatattggc gtgcatcagg acggcctggt tcacatctct tcattgtcga acaagtttgt
    68341 ggaagatccg cataccgtgg tgaaagcggg cgacattgtg aaggtgaaag tgctggaagt
    68401 ggatcttcag cgtaaacgta tcgccctgac catgcgtctg gacgagcagc ctggcgaaac
    68461 caacgcccgt cgcggcggcg gtaatgaacg cccgcaaaac aaccgcccgg cagccaaacc
    68521 acgcggtcgt gaagcgcagc ctgccggtaa tagcgcgatg atggatgcgc tggcggcggc
    68581 aatgggcaaa aaacgttaaa cgccctgatt gacccctaaa cctgcgcgat aaggtgtcca
    68641 cgccgcatct ggcaacgaac gccttgtctg acataaaagt gccggagaat atctccggca
    68701 tttttattcc acagccaaac tcacaatata ttccggcaac atttatcact tcattaacaa
    68761 ctgaaacctt aattaaacat tagccagtcc tggtaattcg ctattcgaat tatattttcg
    68821 ctgcgatata accttgagcc acatcaacat tgagtcagat tattattcaa accaacattc
    68881 gcacacattt taagtattgc tgatagaaac cattctcatt atcattgtgc tgttgattat
    68941 ttaatctctc cttcgttggc aaatcatctg gtctcatgtc gctgtcaaac gccccatgag
    69001 gtagttatcc agttaatgag aaacaagtag gcacctatgc aatacactcc agatactgcg
    69061 tggaaaatca ctggcttttc ccgtgaaatc agcccggcat atcgccaaaa actgctttct
    69121 cttggcatgt tacctggctc ctcttttaat gtggtgcgcg tcgctccact cggcgacccc
    69181 attcatatcg aaacccgtcg tgtgagcctg gtattacgca aaaaagatct ggccttatta
    69241 gaagtggaag cggtttcctg ttaatacggt gataacaaca atgaaaaaat taaccatagg
    69301 cttaattggt aatccaaatt ctggcaagac aaccttattt aaccagctca ctggcgcacg
    69361 tcagcgtgta ggtaactggg ctggcgttac cgtcgaacgt aaagaagggc aattctccac
    69421 caccgatcat caggtcacgc tggtggacct gcccggcacc tattctctga ccaccatctc
    69481 atcgcagacc tcgctcgatg agcaaatcgc ctgtcactac attttgagtg gcgacgccga
    69541 cctgctgatt aacgtggtgg atgcgtctaa ccttgagcgt aacctgtacc tgacgctaca
    69601 actgctggaa ctcggcattc cctgcatcgt ggcactgaac atgctcgaca ttgccgagaa
    69661 gcaaaatatt cgtattgaaa ttgatgctct gtcggcgcgt cttggctgcc cagtgatccc
    69721 gctggtttca acccgtggtc gcggtattga agcgctcaag ctggcgattg atcgctataa
    69781 agctaacgag aatgtggaac tggtgcatta cgcacagccg ctactcaacg aagcagattc
    69841 actggcaaaa gtgatgcctt ccgacatccc gctgaaacaa cgtcgctggc tgggcctgca
    69901 aatgctggaa ggcgatatct acagccgcgc ctacgccggt gaagcgtcgc agcatctgga
    69961 tgccgccctc gcccgtctgc gtaatgagat ggacgatccg gcgctgcaca ttgccgatgc
    70021 gcgttaccag tgcattgctg ccatctgtga tgtggtaagc aacaccctga cggcagaacc
    70081 cagccgtttc accactgcgg tagataaaat cgtgctcaac cgtttcctcg gtctgccgat
    70141 tttcctcttt gtgatgtacc tgatgttcct gctggctatc aacatcggcg gggcgttaca
    70201 gccgctgttt gacgtcggct ccgtggcgct atttgtgcat ggtattcaat ggattggcta
    70261 cacgctccac ttcccggact ggctgactat cttcctcgcc cagggcctgg gtggcggcat
    70321 taacaccgtg ctgccactgg tgccgcagat tggcatgatg tacctgttcc tctccttcct
    70381 tgaggactcc gggtatatgg cgcgtgcggc gtttgtgatg gaccgtctga tgcaggcgct
    70441 gggcttgccg gggaaatcct ttgtgccgct gatcgtcggt ttcggttgta acgtaccgtc
    70501 ggtaatgggt gcacgtacgc ttgatgcacc gcgtgaacgt ctgatgacca tcatgatggc
    70561 accgtttatg tcctgcggcg cgcgtctggc tatcttcgca gtattcgcgg ctgccttctt
    70621 cgggcagaac ggtgcgctgg cggtcttctc gctgtatatg ctgggtattg tgatggcggt
    70681 gctgactggc ctgatgctca agtacaccat catgcgcggt gaagcgacgc cgtttgtcat
    70741 ggagttgccg gtctatcatg taccacacgt taaaagcctg attatccaga cctggcagcg
    70801 tctgaaaggc ttcgttctgc gtgctggtaa agtgatcatc atcgtcagca ttttcctgag
    70861 cgctttcaac agcttctcgc tgagcgggaa aatcgtcgat aacatcaacg actcggcgct
    70921 ggcgtccgtc agccgggtga tcaccccggt cttcaagcca attggcgtgc atgaagataa
    70981 ctggcaggca acggttggcc tgtttacagg tgccatggcg aaagaagtgg tagtgggtac
    71041 gctcaacacc ctctacaccg cagaaaatat tcaggacgaa gagttcaatc cggcagagtt
    71101 taacctcggt gaagagctgt tcagtgcgat agatgaaacc tggcagagcc tgaaagacac
    71161 cttcagcctt agcgtactga tgaaccccat tgaagccagc aaaggcgacg gcgaaatggg
    71221 taccggggcg atgggcgtga tggatcagaa attcggtagc gcagcagcag cttacagcta
    71281 cctgattttc gtcctgctgt atgtaccatg tatctcggtg atgggagcca tcgcccgtga
    71341 atcaagccgt ggctggatgg gcttctccat cctgtggggg ctgaatatcg cttactcgct
    71401 ggcaacattg ttctatcagg tcgccagcta cagtcagcac ccaacttaca gcctggtgtg
    71461 cattctggcg gttatcctgt ttaacatcgt ggttatcggt ctgctgcgcc gcgcgcgtag
    71521 ccgggtggat atcgaactgc tggcaacccg caagtcggta agcagttgct gcgcagccag
    71581 caccaccggt gattgccatt aatggcttca cttattcagg tgcgcgattt gctggcgtta
    71641 cggggccgta tggaagcggc ccagataagc cagacattga acactccaca gccaatgatt
    71701 aacgccatgc tgcaacaact ggaaagtatg ggcaaagccg tgcggattca ggaagaacct
    71761 gacggctgcc tctctggcag ttgtaaaagc tgcccggaag gaaaagcctg tctgcgcgag
    71821 tggtgggcgc tgcgttaacc ttactccatc gcctgttttg gaaagcccgg tatgcgtctg
    71881 catccgggct tttttgcgtg cggctttcca taaaaatgca actcttgcag cacggcgtaa
    71941 gttcctttga aagcatctcg cagggatgaa aactcgctaa tacacaggtg tggagtggcg
    72001 cgtagagtcg cggcattcaa acaacaggtg aaggaacgcc atgagcaaaa agcagagttc
    72061 caccccacac gatgcgctgt tcaaactctt tttacgccaa ccggacacgg ctcgtgattt
    72121 tcttgcgttt catttaccgg cacccattca cgcgctttgt gatatgaaaa ccctcaagct
    72181 ggagtcgagc agctttattg atgacgatct gcgtgaaagc tattccgatg tgctgtggtc
    72241 ggtgaaaacg gaacaaggac caggatacat ctattgtctg attgaacatc aaagcacctc
    72301 aaacaaactg atcgcatttc gcatgatgcg ttacgctatt gccgcaatgc aaaatcacct
    72361 tgatgctgga tacaaaacgt tgccgatggt ggtgccattg ttgttttacc acggtattga
    72421 aagcccctat ccctattcgc tgtgttggct ggattgtttc gccgatccca aactggcaag
    72481 gcagctttat gcctccgcat ttccgctgat tgatgtcacc gtcatgcctg atgatgaaat
    72541 catgcagcac cgacgtatgg cgctgctgga gttaattcaa aaacatattc gtcaacgcga
    72601 tctgatgggg ctggtagagc aaatggcctg cttattaagt agtggatacg ctaatgacag
    72661 acaaatcaaa gggctgttta attacatact gcaaaccggc gacgcggtac gttttaacga
    72721 ttttatcgac ggcgttgccg aacgttcacc gaaacacaag gagagtttaa tgacaattgc
    72781 ggaaagattg cggcaggagg gggaacaatc caaagccctg catatagcca aaataatgct
    72841 tgaatccgga gtccctctta cagacatcat gcgctttacc ggggtgtcag aagaagagtt
    72901 ggctgcggcg agccagtaaa tttctgtctc gccatttcaa aagttgccta caccctctgc
    72961 ttcaacgcca ccagcaggtg acaaaactcg accggatgcg aaataaacgg cgcatgggcc
    73021 gctttggcga agatatatga ttcgctgtga ggccaaagtt tatccagcat cggcaccact
    73081 ttgcgcggca ccagaccgtc gagatagcca tacaatcgca aaaacggcat ggacacgttt
    73141 tgcagcggct gacggagatc gaccgttttc aggatttcca gcccgccatt aagcacgtca
    73201 acctccggca tcggtaacgc cagaacggtt ttcttcaacg cccgcgcatc ctggcgcgcc
    73261 gtttcagtcc ccatggtttg taacgccagg aaccgctcca ctgtacgctg aaaatcatca
    73321 ctgagttgct gctgaaatcc cgccagcacg tccggtttta tccccggcca ctcgtcacga
    73381 gcactaaaac aaggtgacga cgccacggtg accagcgcct gaacacgctc gggatgggtt
    73441 aaaacaattt ggcttgccac cagcccgccc agactccagc ctaaccaaat ggctttatca
    73501 ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg acagcgcacc aaatccccgg
    73561 ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa aatgcgagct aagttcctcg
    73621 tcaatgcaac gccacacttc ggcattcagt ccccatccgt gcagcagcac aagatgaaca
    73681 ttcccctgac ctttggtctg ccaccagatg ttattcatcc gctattgttc tcttttgact
    73741 tacaaggatg aacatatgct aacagtaccg ggattatgct ggctatgccg aatgccactg
    73801 gcgttaggtc attgggggat ttgttcggtc tgttcacgcg ccacccgcac agataaaacg
    73861 ttatgcccac aatgtggatt acccgccaca cactcccatc ttccctgcgg tcgctgcctg
    73921 caaaaaccgc cgccctggca aagactggtc acggttgccg actatgcgcc gccgttaagt
    73981 ccgcttatcc accagcttaa attttcccgg cgcagtgaaa tcgccagcgc cctgtcacgt
    74041 ctgttactat tggaagtctt acacgctcgt cgcaccaccg ggttgcaatt gccggatcgc
    74101 atcatcagcg ttccgttatg gcagcggcgt cactggcgtc ggggatttaa tcagagcgat
    74161 ttgctgtgtc agccgttatc acgctggttg cattgccaat gggatagcga agccgtcaca
    74221 cgtacacggg ccactgcgac ccagcatttt ctcagtgccc ggctgcgcaa gcgcaacctg
    74281 aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc atatggtgat tgtggatgat
    74341 gtcgttacca ccggaagtac cgtcgcagag attgcgcagt tgcttttacg caatggtgcg
    74401 gcgactgtcc aggtctggtg cctttgtcga accttgtaga gcctcgatga tgggcgtatt
    74461 ataaccaact aaaatagtca actattaggc cattactatg atccgtattt ccgatgctgc
    74521 acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa gggacacaaa tccgcgtatt
    74581 tgtgattaac cctggcacgc ctaacgctga atgtggtgtt tcttattgtc cgccggatgc
    74641 cgtggaagcc accgacactg ccctgaaatt tgacctgctg accgcgtatg ttgatgagtt
    74701 aagcgcacca tacctggaag atgcagagat cgactttgtt accgaccagt tgggttctca
    74761 gttaacgctg aaagccccga acgccaaaat gcgtaaagtg gcagacgatg caccgctgat
    74821 ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca cagcttgctg gtcacggtgg
    74881 tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc attctgcaat ttggcggcgg
    74941 ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa gggatcgaga agcaactgct
    75001 gaacgaattc ccggagctga aaggtgtgcg cgatctcacc gaacaccagc gcggcgaaca
    75061 ctcctactac taagttatcc cctcttttgt agattgcccg atgcgacgct aaagcgtcat
    75121 ttcgggccta cgttgaacat gtgccagacg ttggcacagc attgctttgg caaatccctc
    75181 ccttcgtttc cccgcgataa tatgaccaac ctctcataat ttaaatttac cccgctctgg
    75241 tgattctcaa acgccagatg ttacccgtat cattcacatg ggtaccaaac atactcctga
    75301 catctgacta caataattag ttttagtggg tatcagtcgt ggtgccgcaa tatctctgtt
    75361 cccgattggg ataattagag tttgtcgtca gaaaattgac gttacccata acaaatgaaa
    75421 ggccaggtaa atcatgccat tagtcattgt tgctatcggt gtaatcttgt tgttgctcct
    75481 gatgatccgc ttcaaaatga acggcttcat cgctctcgtc ctcgtggcgc ttgctgttgg
    75541 attaatgcaa ggaatgccgc tggataaagt tattggctcc atcaaagccg gtgtcggcgg
    75601 gacgctcggt agccttgccc tgatcatggg ttttggcgca atgctgggca aaatgctggc
    75661 agactgcggt ggcgcacaac gtatcgccac cacgctgatt gccaaatttg gtaaaaaaca
    75721 catccagtgg gcggtggtac tgaccggttt taccgttggt tttgccctgt tctatgaagt
    75781 gggctttgtg ctgatgctgc cgctggtgtt taccatcgcg gcttctgcga atattccact
    75841 gttgtatgtt ggtgtaccaa tggcagctgc actgtctgtg acccacggct tcctgccacc
    75901 gcatccgggt ccgactgcga ttgccaccat tttcaatgcc gatatgggta aaaccctgct
    75961 gtacggtact attctggcaa tcccgaccgt gattctcgcc ggtccggttt acgctcgcgt
    76021 gttgaaaggt atcgataagc caattccgga aggtctctac agcgcgaaaa ccttcagcga
    76081 agaagagatg ccgagctttg gcgtcagcgt ctggacctct ctggtgccgg tagtactgat
    76141 ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt cacgctttcc tgccggtagc
    76201 ggagttcctc ggtgacccgg taatggctac gctgattgcc gtgctgattg ccatgttcac
    76261 ctttggtctg aaccgtggtc gttcaatgga tcagattaac gacacgctgg tttcttccat
    76321 caaaatcatt gcgatgatgc tgttgatcat cggtggtggc ggtgcgttca agcaggtgct
    76381 ggtagacagc ggcgtggaca aatacattgc ttccatgatg cacgaaacca acatttctcc
    76441 gctgctgatg gcctggtcga ttgctgccgt actgcgtatc gcgctgggtt ccgcaaccgt
    76501 tgcggcaatc actgcgggtg gtatcgcggc accgctgatt gcaacgacgg gtgttagccc
    76561 ggagctgatg gttattgcgg tgggttccgg tagtgtgatt ttctctcatg tgaacgatcc
    76621 gggcttctgg ctgttcaaag agtactttaa cctgactatc ggcgagacca tcaaatcctg
    76681 gtcgatgctg gaaaccatta tctcggtgtg cggtctggta ggctgtctgc tgctgaatat
    76741 ggtgatttga taacactgac tgccggatgc atcgcatccg gcatcaaccg cactctactt
    76801 cttcttcgct gcagctctgc gccgtctgtc caaatccttc agcaacttgt tcacgccatc
    76861 atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt cgccagtttt tatactggta
    76921 actggtgcca ggaatattca ccggttcggc catatccagc cagtcttccg gctgtagtcc
    76981 taacagagca ctgttactgt cggcaatgta gcgctgcaaa ccacggttca gcgtcggcgt
    77041 catcgacatc aacgatgcct tatgcccggc acgttttggc agacaaccat atttatgcag
    77101 tgcatccagc agcccttgct tcgccagttc gcgatcctga tacagaccgc gcagtaccac
    77161 ttcatccgga tacagcccca gggttttgcc cagcgttaga tccccgctct cccagtaacc
    77221 gcgtagcgta ggcaggtcat gtgtcgccgc aaccgccatc gactgctccg gatacgcttt
    77281 cggtgcacgg aacgtcttct cgtggtcgtt ttcgaaatag agcactttgt aggagtacac
    77341 accgctgctg cgcagcttac cgacaatctc taccggtacg gtaccgagat cttcaccaat
    77401 caccatacag cgatgacgtt tactttcgag tgccagaatc gagagcagat catccaccgg
    77461 atagtgaaca tacgcgccct gatctgccgt ctcgccatac ggtatccacc acaaacgcag
    77521 catcgacatc acatggtcaa ttcgtaatgc gccgcagttt tgcatattgg cacgcaacag
    77581 ctcgataaac ggttcatagg cacgcgcggt gatgatatgc gggtccattg gcggtaatcc
    77641 ccagttctgc cccaacgggc cgaggatatc cggcggcgcg ccaaccgacg ctttcaggca
    77701 atatagttca cggtcacacc aggtttccgc cccaccttcc gctacgccaa ccgccagatc
    77761 acgatacaag ccaatcggca tttcatagcc ctggcttatc tcccagcagg cggcaaactg
    77821 gctgtaagcc agccactgca accagagata aaaatcgacg tcatcacgat gttcttcgca
    77881 gaactgacgc acttctggtg aatccacgtt ctgatacatc tctggccatg caggccagcc
    77941 ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc gcatcaaagg ctgcctgcca
    78001 gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac gcggtcatct gctcatcatc
    78061 acgttgcgcg aaacctttcc acgccattcg taatgctgtc atttttaggg cggtaaccgt
    78121 ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc gtctgttgcg tggtcggcaa
    78181 ctgccaccag gcctgagcct cttcgctaag atggaaatct tcaacggcgt taacgtcgat
    78241 ataaatcaca ttcagccaac ggcgagaaga cgggctgtat gggctggcac tctccggatt
    78301 tgccggatag agcgcatgaa tcgggttcag gccgatgaac gacccgccac gttttgccac
    78361 atccaccagc atcgctttga gatcgccaaa atccccaata ccccagtttt tttccgatcg
    78421 cagcgtataa agctgaacgc aggcacccca cagcttttgt ttattcagca acgcctgcgg
    78481 ttcgtaacag cgtttcgggg cgacaatcac ccggcaatgc gcgcgctggt cgtcctgggt
    78541 gagtgtcagc gtgtgataac cttccggcag cttcgtcggt agattgaacg ctttgccccc
    78601 cgttacatgg cctttgtact gtgttccttc ttcggtggtc agcagccagc tatattcgcc
    78661 gctgccctcc accaccatcg gcattttttt gccgctggta taaaccatga cattcgggac
    78721 tggcgttacc gccactttcg tggcggtacg ttgatgcatc gcgtcaagca aacgccgttt
    78781 ggtttcggcg ctaatcgact gcggtttacc gtgggcattg atgtaattgg ggctaatccc
    78841 cgccgccagc gcggcattat ccagacgttt gctttccatc gagcttcctt agcgttttgc
    78901 ctgccagata cgagcctgat aatcgcgaat agagcgatcc gagctaaaca taccgcagcg
    78961 ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg tcgcggtaca gcacatccac
    79021 ctgcttttgt gcctcaacat aggctgcgaa atccgccatc accagatacg gatcgccgcc
    79081 ttgtttgccg atactgtgca gcatctggtc gaaggcatgc ttatcgccgt cgctgtattt
    79141 accgctttcc agctctttca atactgcgtc cagcacctta tctttcttcc gccatttcac
    79201 cgggtcgtag cctttggcca gaatagcctt cacttgttcc acggtatgac caaaaataaa
    79261 gatattttct tcaccgactt tctcggcgat ttcaacgttc gcaccatcca gcgtaccgac
    79321 agtaagcgca ccattgagcg ccagtttcat attgccggta ccggaagctt ctttacctgc
    79381 agtcgaaatt tgttcggaga tatccgccgc cgggatcagt ttttccgccg ccgaaacgca
    79441 ataatccggc aggaacacca ccttcaactt atcgccaacc agtggatcgt tgttgatcac
    79501 gtcagccact ttgttgatcg caaagataat attcttcgcc aggtagtagc ccggtgccgc
    79561 tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc tgcgggtttt cacgaatttc
    79621 tttgtacaac gccagaatat gcagcagatt caggtgctgg cgtttgtact catgcagacg
    79681 tttgatctga atatcgaaaa tcgcctgtgg attgatctca ataccggtac gaactttcac
    79741 aaactccgcc agacggactt tattcgcctg cttgatctcg cgatattgct gacggaattt
    79801 cgcatcatca gcgaattttt ccagattgat cagctgatcg agatcgttag cccactcttt
    79861 ttgcagtgat ttatccaaca gagccgccag tgcagggttg cactgtttga tccagcgacg
    79921 tggggtaata ccgttggtga cgttatggaa tttgttcggc catagctggt gatattccgg
    79981 gaacagatct ttcaccacca gatccgagtg cagcgccgca acaccgttca ccgcgaaacc
    80041 gccaaccaca cacaggttcg ccatatgcac ttgtttatcg tgcaccaccg ccagtttggc
    80101 ccacactttt tcatcgcccg gccaggtttt ctccaccagc gttttaaagc gagtattaat
    80161 ttcgttaata atctgcatat ggcgcggcag taagcctttc accagtttca catcccagcg
    80221 ttccagcgct tctggcatca gggtatggtt ggtgtaagcg aaagttttgc tggtaatggc
    80281 ccaggcgtca tcccagctca tctggtgctc atcgatcagc acgcgcagca gttctggaat
    80341 cgcgatagtt gggtgggtat cgttcagctg aataacttcg taatccgcca gttcgtgcag
    80401 tttacgcccc gccagatgat ggcgacgcaa aatatccgct accgaacagg cacactggaa
    80461 gtactgctgc atcaagcgca gctttttacc ggcagtatgg ttgtcgtttg gatagagaac
    80521 tttggtcagt ttttccgcat tgatgccctg ctgttcggca cgcaggaaat caccgtcgtt
    80581 aaatttagtc agatcaaacg gatgcgcgtg cgtcgcctgc cacagacgca gcggctgcgc
    80641 cacgccatta cgatagccga caacggggag atcccacgct tgaccggtaa tggtaaactc
    80701 cggctcccag cgtccgtctt tcgtcacttt accgccaatc cctacctgca catccagtgc
    80761 ttcgttgtgg cggaaccacg ggtagttact gcgatgccag tcatccggcg cttcaacctg
    80821 tttgccatcg acaaaagact ggcggaacaa accatattga tagttcagac cgtaacccgt
    80881 cgcagactga ccgacagttg ccattgagtc gaggaagcac gccgccagac gtcccagacc
    80941 accgttaccc agcgccgggt cgatctcttc ttccagcagg tccgtcagat tgatgtcata
    81001 agccttcaac gaatcctgta catcctgata ccagccgaga ttcaacaggt tgttgcccgt
    81061 caggcgacca atcaaaaact ccattgagat gtagttaaca tgtcgctgat tcgccaccgg
    81121 cttggcgaat ggctgagcac gcagcatttc ggccagtgct tcactcactg ctagccacca
    81181 ctggcgagga gtcatttcag ccgcagaatt taagccataa cgctgccact gacgtgaaag
    81241 cgcttcctga aattgcttat cgttaaaaat aggttgtgac ataggagttc cacttttctt
    81301 agattttcaa cacaacgtta tcgctagttt gccaggctcg atgttgacct tcctcatcct
    81361 gcgggggatt aggcagggag gagttgcggg gatgagcaag gaaatgtgat ctcaaccact
    81421 taaagctagt gcaaaccaca ggattagcag caaatcaatg caatacagcg cagaaaatct
    81481 gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa agcggaaggt aactctataa
    81541 attaagtaaa ggagtgaaac agtttcataa gtaaaatatc cagtgtgctc catctcattc
    81601 ttaatagatt tattaagatc atctttttag atggcacttt catcaggaat gaagaagaaa
    81661 cccttgctta aatgaatctg atgaacataa gggaaaccag tattcacgct ggatcagcgt
    81721 cgttttaggt gagttgttaa taaagatttg gaattgtgac acagtgcaaa ttcagacaca
    81781 taaaaaaacg tcatcgcttg cattagaaag gtttctggcc gaccttataa ccattaatta
    81841 cgaagcgcaa aaaaaataat atttcctcat tttccacagt gaagtgatta actatgctga
    81901 ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac cgtggttcgt gagcgcctgc
    81961 tggctaaact ttccggcgcg aacaacttcc ggctggcgct gatcacgagt cctgcgggct
    82021 acggaaagac caccctcatt tcccagtggg cggcaggcaa aaacgatatc ggctggtact
    82081 cgctggatga aggtgataac cagcaagagc gtttcgccag ctatctcatt gccgccgtgc
    82141 agcaggcaac caacggtcac tgtgcgatat gtgagacgat ggcgcaaaaa cggcaatatg
    82201 ccagcctgac gtcactcttc gcccagcttt tcattgagct ggcggaatgg catagcccac
    82261 tttatctggt catcgatgac tatcatctga tcactaatcc agtgatccac gagtcaatgc
    82321 gcttctttat tcgccatcaa ccagaaaatc tcaccctggt ggtgttgtca cgcaaccttc
    82381 cgcaactggg cattgccaat ctgcgtgttc gtgatcaact gctggaaatt ggcagtcagc
    82441 aactggcatt tacccatcag gaagcgaagc agttttttga ttgccgtctg tcatcgccga
    82501 ttgaagctgc agaaagcagt cggatttgcg atgacgtttc cggttgggcg acggcactac
    82561 agctaatcgc cctctccgcc cggcagaata cccactcagc ccataagtcg gcacgccgcc
    82621 tggcgggaat caatgccagc catctttcgg attatctggt cgatgaggtt ttggataacg
    82681 tcgatctcgc aacgcgccat tttctgttga aaagcgccat tttgcgctca atgaacgatg
    82741 ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat gcgtctcgaa gagattgagc
    82801 gtcaggggct gtttttacag cggatggatg ataccggcga gtggttctgc tatcacccgc
    82861 tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact ggcggcggag ctgccggaaa
    82921 tccaccgtgc cgccgcagaa agctggatgg cccagggatt tcccagcgaa gcgattcatc
    82981 atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat tctgcttaat cacgcctgga
    83041 gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc gcttaaggcc ctgccgtggg
    83101 acagtttgct ggaaaatccg cagttagtgt tattgcaggc gtggctaatg caaagccaac
    83161 atcgctatgg cgaagttaac accctgctag cccgtgctga acatgaaatc aaggacatca
    83221 gagaaggcac catgcacgca gaatttaacg ctctgcgcgc ccaggtggcg attaacgatg
    83281 gtaatccgga tgaagcggaa cggctggcaa aactggcact ggaagagctg ccgccgggct
    83341 ggttctatag ccgcattgtg gcaacctcgg tgctgggtga agtgctgcac tgcaaaggcg
    83401 aattgacccg ctcactggcg ctaatgcagc aaaccgaaca gatggcacgc cagcacgacg
    83461 tctggcacta cgccttatgg agtttaatcc agcaaagtga aattctgttt gcccaagggt
    83521 tcctgcaaac cgcgtgggaa acacaggaaa aagcgttcca gctgatcaac gagcagcatc
    83581 tggaacagct gccgatgcat gagtttctgg tgcgcatccg agcacaactg ttatgggcct
    83641 gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg gattgaagtc ttgtcgtctt
    83701 atcagccaca gcaacagctt cagtgcctgg caatgttgat tcaatgctcg ctggcccgtg
    83761 gtgatttaga taacgcccgt agccagctga accgtctgga aaacctgctg gggaatggca
    83821 aatatcacag cgactggatc tctaacgcca acaaagtccg ggtgatttac tggcaaatga
    83881 ccggcgataa agccgccgct gccaactggt tgcgtcatac ggctaaaccg gagtttgcga
    83941 acaaccactt cctgcaaggt caatggcgca acattgcccg cgcacaaatc ttgctgggcg
    84001 agtttgaacc tgcggaaatt gttctcgaag aactcaatga aaatgcccgc agtctgcggt
    84061 tgatgagcga tctcaaccgt aacctgttgc tgcttaatca actgtactgg caggccggac
    84121 gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa actggcgaat cgcaccggat
    84181 ttatcagcca ttttgtcatc gaaggcgaag cgatggcgca acaactgcgc cagctgattc
    84241 aactcaatac gctgccggaa ctggaacagc atcgcgcaca gcgtattctg cgagaaatca
    84301 atcaacatca tcggcataaa ttcgcccatt tcgatgagaa tttcgttgaa cgtctgctaa
    84361 atcatccgga agtgccagaa ctgatccgca ccagcccgtt gacccaacgt gaatggcagg
    84421 tcctggggct gatttactcc ggttacagca atgaacaaat tgcgggcgaa ctggaagtgg
    84481 cggcaaccac catcaagacg catatccgca atctgtacca aaaactcggc gtcgcccacc
    84541 gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat gatgggttat ggggtgtgag
    84601 tttagccgga taatgcattg gatccggttt acatctttac atcattcaat actcacccgc
    84661 gttacgccat ctgtttctat caaaccaaac cgcaccggca agaaacgctc caccaccgcg
    84721 atattggtca gcagatggca tgaaggatgg gcgaccttaa attctcccgc gcccgccagc
    84781 gccatcggca gtaccagttg gtcggcgaga tattccccca ccgccgctgg gcttgccagg
    84841 tagcgtttca cctctttcac taactgcgcg gctaccacct cggcactgac gcgcttttca
    84901 ccgacgacga aaaagcgttc agtgatattt tcactttcga cttccagcga gacggtatta
    84961 cccggcccct ggtcgcgcgg caggttatga atattctgtt catgcaggga aaaactcccc
    85021 gccagtgtag cgatttcacg ctcagcaaca tggcgcggca caccagctaa tagaacttct
    85081 ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca aggtgttaaa cgatgccacg
    85141 ggcgagactt ccgttgccac cacgccgcct ccggcaggat aaaaaccgtg acgtaacagc
    85201 gtggtttgct gatgaattcc tattttcgcc agcagcggct ccagcacccg gcggataaaa
    85261 tccgcaggcg gggccgacgg gttatcggtg cctccgctca cttcaacacg cgaaggtcca
    85321 tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca gcgtacagct tccggcacta
    85381 ccgatagcaa agcggtactc gccgccgcgc acggtgccag gccggaagac cagacgctgc
    85441 gaccccagct ccgcgccttc caccgttgcc ctacaaattt ccgcagccgc ttttaccgcg
    85501 gtcagatgct ggcgcaacag ccccggtttc gcccgcccgg cacgaatgct ggtgatggta
    85561 aatggctggc cggttatcat cgacaggctc agcgccgagc gcaggatctg cccgccacct
    85621 tcgccctgtg cgccatccag cgcaatcatc cttttcatca tttatccttt tacgcacacc
    85681 acctgacgca gggtatagat aacttccacc agatcgcttt gtgccgccat caccgcatca
    85741 atatctttat acgccatcgg gatttcgtcg atcacttcgg catctttacg gcattccaca
    85801 tgcgcggtgg cacgaatttg atcttccacg ctgaacagtt ttttcgcttt agtacggctc
    85861 attacccgcc cggcaccgtg gctgcacgaa cagaacgact cttcatttcc cagcccacgg
    85921 acgataaagc tttttgctcc catcgaaccg ggaataattc catattgacc agcacgcgca
    85981 gacaccgcgc ctttacgcgt cacgtagatc tcttcaccaa agtgctgttc tttttgcaca
    86041 tagttgtggt gacagttgat ctcttccatc gccagcgttt gtggctgtct gaccgttttc
    86101 tgcgtaatgc tctgcaacgc cgttaccacg ttttccatca tcgcatcgcg gttaaggctg
    86161 gcgaaaagct gcgcccaggc cacggctctt aggtaatcat caaagtattc cgtaccttcc
    86221 ataaagtacg ccaggtcgcg cgatggcaac gtttcaagct ggtcctgcat ctctttttgt
    86281 gccaaatcga taaagtaagt cccgatggcg ttaccaattc cgcgtgaacc ggagtgcagc
    86341 ataatccaca cctggtccga ctcatcaagg cagatttcaa taaagtggtt acccgttccc
    86401 agcgttccca ggtgtttata gttgttagtg ttaaggaaac gcggatattt ttgcgttaac
    86461 cactgataac cggcttcaag ctcagcccat ttcgcatcga cgttaacagg cgggttttcc
    86521 catgcgcctt tatcacgttt acaacggcca gtggtacgtc cgtgcggcac cgcagtttca
    86581 atcgcctgac gcagctctgc caggttttca ggcaggtctg ccgccgttaa cgcggtacgc
    86641 agcgcgttca ttccacagcc aatatccacg cccaccgccg ccggaataat cgcccctttg
    86701 gtcgggatca cgctaccaat ggtggaacct ttacccaggt gtacatcagg cattaccgca
    86761 atatgtttga aaataaacgg catcttcgcc gtattaataa gttgctgacg cgcatcggct
    86821 tctaccggca cgcctttggt ccacattttt accggggcat tttcagtggt cagtaattcg
    86881 taattcattt tgtttttctc ttttcgttgt ttgctgtcgt gataat
//
LOCUS       DAZGTR010000006        86421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGTR010000006 DAZGTR010000000
VERSION     DAZGTR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 86421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.006"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1013)
                     /locus_tag="ACHLCY_000456"
     CDS             complement(<1..1013)
                     /locus_tag="ACHLCY_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0367778.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRG"
     gene            complement(1472..1558)
                     /locus_tag="ACHLCY_000457"
     tRNA            complement(1472..1558)
                     /locus_tag="ACHLCY_000457"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1522..1524),aa:Leu,seq:gag)
     gene            complement(1573..1905)
                     /gene="secG"
                     /locus_tag="ACHLCY_000458"
     CDS             complement(1573..1905)
                     /gene="secG"
                     /locus_tag="ACHLCY_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP0367779.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(2133..3470)
                     /gene="glmM"
                     /locus_tag="ACHLCY_000459"
     CDS             complement(2133..3470)
                     /gene="glmM"
                     /locus_tag="ACHLCY_000459"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP0367780.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(3463..4311)
                     /gene="folP"
                     /locus_tag="ACHLCY_000460"
     CDS             complement(3463..4311)
                     /gene="folP"
                     /locus_tag="ACHLCY_000460"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP0367781.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(4401..6335)
                     /gene="ftsH"
                     /locus_tag="ACHLCY_000461"
     CDS             complement(4401..6335)
                     /gene="ftsH"
                     /locus_tag="ACHLCY_000461"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP0367782.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(6435..7064)
                     /gene="rlmE"
                     /locus_tag="ACHLCY_000462"
     CDS             complement(6435..7064)
                     /gene="rlmE"
                     /locus_tag="ACHLCY_000462"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP0367783.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVNQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            7190..7483
                     /gene="yhbY"
                     /locus_tag="ACHLCY_000463"
     CDS             7190..7483
                     /gene="yhbY"
                     /locus_tag="ACHLCY_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP0367784.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(7639..8115)
                     /gene="greA"
                     /locus_tag="ACHLCY_000464"
     CDS             complement(7639..8115)
                     /gene="greA"
                     /locus_tag="ACHLCY_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP0367785.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDKE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            8363..9796
                     /gene="dacB"
                     /locus_tag="ACHLCY_000465"
     CDS             8363..9796
                     /gene="dacB"
                     /locus_tag="ACHLCY_000465"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0367786.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(9836..11008)
                     /gene="cgtA"
                     /locus_tag="ACHLCY_000466"
     CDS             complement(9836..11008)
                     /gene="cgtA"
                     /locus_tag="ACHLCY_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP0367787.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(11024..11989)
                     /gene="yhbE"
                     /locus_tag="ACHLCY_000467"
     CDS             complement(11024..11989)
                     /gene="yhbE"
                     /locus_tag="ACHLCY_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0367788.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(12116..12373)
                     /gene="rpmA"
                     /locus_tag="ACHLCY_000468"
     CDS             complement(12116..12373)
                     /gene="rpmA"
                     /locus_tag="ACHLCY_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP0367789.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(12394..12705)
                     /gene="rplU"
                     /locus_tag="ACHLCY_000469"
     CDS             complement(12394..12705)
                     /gene="rplU"
                     /locus_tag="ACHLCY_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP0367790.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            12964..13935
                     /gene="ispB"
                     /locus_tag="ACHLCY_000470"
     CDS             12964..13935
                     /gene="ispB"
                     /locus_tag="ACHLCY_000470"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP0367791.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            14162..14440
                     /gene="sfsB"
                     /locus_tag="ACHLCY_000471"
     CDS             14162..14440
                     /gene="sfsB"
                     /locus_tag="ACHLCY_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP0367792.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(14488..15747)
                     /gene="murA"
                     /locus_tag="ACHLCY_000472"
     CDS             complement(14488..15747)
                     /gene="murA"
                     /locus_tag="ACHLCY_000472"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP0367793.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(15802..16056)
                     /gene="ibaG"
                     /locus_tag="ACHLCY_000473"
     CDS             complement(15802..16056)
                     /gene="ibaG"
                     /locus_tag="ACHLCY_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP0367794.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(16216..16509)
                     /gene="mlaB"
                     /locus_tag="ACHLCY_000474"
     CDS             complement(16216..16509)
                     /gene="mlaB"
                     /locus_tag="ACHLCY_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP0367795.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(16509..17144)
                     /gene="mlaC"
                     /locus_tag="ACHLCY_000475"
     CDS             complement(16509..17144)
                     /gene="mlaC"
                     /locus_tag="ACHLCY_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP0367796.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(17163..17714)
                     /gene="mlaD"
                     /locus_tag="ACHLCY_000476"
     CDS             complement(17163..17714)
                     /gene="mlaD"
                     /locus_tag="ACHLCY_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP0367797.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(17719..18501)
                     /gene="mlaE"
                     /locus_tag="ACHLCY_000477"
     CDS             complement(17719..18501)
                     /gene="mlaE"
                     /locus_tag="ACHLCY_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP0367798.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(18509..19318)
                     /gene="mlaF"
                     /locus_tag="ACHLCY_000478"
     CDS             complement(18509..19318)
                     /gene="mlaF"
                     /locus_tag="ACHLCY_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP0367799.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            19528..20505
                     /gene="yrbG"
                     /locus_tag="ACHLCY_000479"
     CDS             19528..20505
                     /gene="yrbG"
                     /locus_tag="ACHLCY_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP0367800.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPSRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            20519..21505
                     /gene="kdsD"
                     /locus_tag="ACHLCY_000480"
     CDS             20519..21505
                     /gene="kdsD"
                     /locus_tag="ACHLCY_000480"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP0367801.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            21526..22092
                     /gene="kdsC"
                     /locus_tag="ACHLCY_000481"
     CDS             21526..22092
                     /gene="kdsC"
                     /locus_tag="ACHLCY_000481"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP0367802.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            22089..22664
                     /gene="lptC"
                     /locus_tag="ACHLCY_000482"
     CDS             22089..22664
                     /gene="lptC"
                     /locus_tag="ACHLCY_000482"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP0367803.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            22633..23190
                     /gene="lptA"
                     /locus_tag="ACHLCY_000483"
     CDS             22633..23190
                     /gene="lptA"
                     /locus_tag="ACHLCY_000483"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP0367804.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            23197..23922
                     /gene="lptB"
                     /locus_tag="ACHLCY_000484"
     CDS             23197..23922
                     /gene="lptB"
                     /locus_tag="ACHLCY_000484"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP0367805.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            23970..25403
                     /gene="rpoN"
                     /locus_tag="ACHLCY_000485"
     CDS             23970..25403
                     /gene="rpoN"
                     /locus_tag="ACHLCY_000485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP0367806.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            25426..25713
                     /gene="hpf"
                     /locus_tag="ACHLCY_000486"
     CDS             25426..25713
                     /gene="hpf"
                     /locus_tag="ACHLCY_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP0367807.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            25831..26322
                     /gene="ptsN"
                     /locus_tag="ACHLCY_000487"
     CDS             25831..26322
                     /gene="ptsN"
                     /locus_tag="ACHLCY_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP0367808.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            26368..27222
                     /gene="rapZ"
                     /locus_tag="ACHLCY_000488"
     CDS             26368..27222
                     /gene="rapZ"
                     /locus_tag="ACHLCY_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP0367809.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            27219..27491
                     /gene="npr"
                     /locus_tag="ACHLCY_000489"
     CDS             27219..27491
                     /gene="npr"
                     /locus_tag="ACHLCY_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP0367810.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            27705..28337
                     /gene="yrbL"
                     /locus_tag="ACHLCY_000490"
     CDS             27705..28337
                     /gene="yrbL"
                     /locus_tag="ACHLCY_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP0367811.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(28334..29062)
                     /gene="mtgA"
                     /locus_tag="ACHLCY_000491"
     CDS             complement(28334..29062)
                     /gene="mtgA"
                     /locus_tag="ACHLCY_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP0367812.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(29059..29712)
                     /gene="elbB"
                     /locus_tag="ACHLCY_000492"
     CDS             complement(29059..29712)
                     /gene="elbB"
                     /locus_tag="ACHLCY_000492"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP0367813.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(29942..32278)
                     /gene="arcB"
                     /locus_tag="ACHLCY_000493"
     CDS             complement(29942..32278)
                     /gene="arcB"
                     /locus_tag="ACHLCY_000493"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP0367814.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(32374..33303)
                     /gene="yhcC"
                     /locus_tag="ACHLCY_000494"
     CDS             complement(32374..33303)
                     /gene="yhcC"
                     /locus_tag="ACHLCY_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP0367815.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            33978..38438
                     /gene="gltB"
                     /locus_tag="ACHLCY_000495"
     CDS             33978..38438
                     /gene="gltB"
                     /locus_tag="ACHLCY_000495"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP0367816.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQRGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            38451..39869
                     /gene="gltD"
                     /locus_tag="ACHLCY_000496"
     CDS             38451..39869
                     /gene="gltD"
                     /locus_tag="ACHLCY_000496"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP0367817.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNPLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            40053..41100
                     /gene="yhcG"
                     /locus_tag="ACHLCY_000497"
                     /pseudo
     CDS             40053..41100
                     /gene="yhcG"
                     /locus_tag="ACHLCY_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcG family protein"
     gene            complement(41160..41624)
                     /gene="nanQ"
                     /locus_tag="ACHLCY_000498"
     CDS             complement(41160..41624)
                     /gene="nanQ"
                     /locus_tag="ACHLCY_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP0367818.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(41621..42496)
                     /gene="nanK"
                     /locus_tag="ACHLCY_000499"
     CDS             complement(41621..42496)
                     /gene="nanK"
                     /locus_tag="ACHLCY_000499"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0367819.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPATFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(42493..43182)
                     /gene="nanE"
                     /locus_tag="ACHLCY_000500"
     CDS             complement(42493..43182)
                     /gene="nanE"
                     /locus_tag="ACHLCY_000500"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP0367820.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(43230..44720)
                     /gene="nanT"
                     /locus_tag="ACHLCY_000501"
     CDS             complement(43230..44720)
                     /gene="nanT"
                     /locus_tag="ACHLCY_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP0367821.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(44830..45723)
                     /gene="nanA"
                     /locus_tag="ACHLCY_000502"
     CDS             complement(44830..45723)
                     /gene="nanA"
                     /locus_tag="ACHLCY_000502"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0367822.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLTGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(45845..46636)
                     /gene="nanR"
                     /locus_tag="ACHLCY_000503"
     CDS             complement(45845..46636)
                     /gene="nanR"
                     /locus_tag="ACHLCY_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0367823.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            47016..48383
                     /gene="dcuC"
                     /locus_tag="ACHLCY_000504"
     CDS             47016..48383
                     /gene="dcuC"
                     /locus_tag="ACHLCY_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0367824.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMSTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(48426..48923)
                     /gene="sspB"
                     /locus_tag="ACHLCY_000505"
     CDS             complement(48426..48923)
                     /gene="sspB"
                     /locus_tag="ACHLCY_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP0367825.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(48929..49567)
                     /gene="sspA"
                     /locus_tag="ACHLCY_000506"
     CDS             complement(48929..49567)
                     /gene="sspA"
                     /locus_tag="ACHLCY_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP0367826.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(49962..50354)
                     /gene="rpsI"
                     /locus_tag="ACHLCY_000507"
     CDS             complement(49962..50354)
                     /gene="rpsI"
                     /locus_tag="ACHLCY_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP0367827.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(50370..50798)
                     /gene="rplM"
                     /locus_tag="ACHLCY_000508"
     CDS             complement(50370..50798)
                     /gene="rplM"
                     /locus_tag="ACHLCY_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP0367828.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(51017..52144)
                     /gene="zapE"
                     /locus_tag="ACHLCY_000509"
     CDS             complement(51017..52144)
                     /gene="zapE"
                     /locus_tag="ACHLCY_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP0367829.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            52338..52736
                     /gene="zapG"
                     /locus_tag="ACHLCY_000510"
     CDS             52338..52736
                     /gene="zapG"
                     /locus_tag="ACHLCY_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP0367830.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            52890..54257
                     /gene="degQ"
                     /locus_tag="ACHLCY_000511"
     CDS             52890..54257
                     /gene="degQ"
                     /locus_tag="ACHLCY_000511"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP0367831.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            54347..55414
                     /gene="degS"
                     /locus_tag="ACHLCY_000512"
     CDS             54347..55414
                     /gene="degS"
                     /locus_tag="ACHLCY_000512"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP0367832.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(55477..56415)
                     /gene="mdh"
                     /locus_tag="ACHLCY_000513"
     CDS             complement(55477..56415)
                     /gene="mdh"
                     /locus_tag="ACHLCY_000513"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0367833.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            56850..57320
                     /gene="argR"
                     /locus_tag="ACHLCY_000514"
     CDS             56850..57320
                     /gene="argR"
                     /locus_tag="ACHLCY_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP0367834.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            57685..57948
                     /gene="yhcN"
                     /locus_tag="ACHLCY_000515"
     CDS             57685..57948
                     /gene="yhcN"
                     /locus_tag="ACHLCY_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP0367835.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(58004..58276)
                     /gene="yhcO"
                     /locus_tag="ACHLCY_000516"
     CDS             complement(58004..58276)
                     /gene="yhcO"
                     /locus_tag="ACHLCY_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP0367836.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(58368..60335)
                     /gene="aaeB"
                     /locus_tag="ACHLCY_000517"
     CDS             complement(58368..60335)
                     /gene="aaeB"
                     /locus_tag="ACHLCY_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP0367837.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVEIAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(60341..61273)
                     /gene="aaeA"
                     /locus_tag="ACHLCY_000518"
     CDS             complement(60341..61273)
                     /gene="aaeA"
                     /locus_tag="ACHLCY_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP0367838.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(61281..61484)
                     /gene="aaeX"
                     /locus_tag="ACHLCY_000519"
     CDS             complement(61281..61484)
                     /gene="aaeX"
                     /locus_tag="ACHLCY_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP0367839.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            61667..62596
                     /gene="aaeR"
                     /locus_tag="ACHLCY_000520"
     CDS             61667..62596
                     /gene="aaeR"
                     /locus_tag="ACHLCY_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP0367840.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(62724..64169)
                     /gene="tldD"
                     /locus_tag="ACHLCY_000521"
     CDS             complement(62724..64169)
                     /gene="tldD"
                     /locus_tag="ACHLCY_000521"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP0367841.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(64325..68125)
                     /gene="yhdP"
                     /locus_tag="ACHLCY_000522"
     CDS             complement(64325..68125)
                     /gene="yhdP"
                     /locus_tag="ACHLCY_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP0367842.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(68193..69662)
                     /gene="rng"
                     /locus_tag="ACHLCY_000523"
     CDS             complement(68193..69662)
                     /gene="rng"
                     /locus_tag="ACHLCY_000523"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP0367843.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(69652..70245)
                     /gene="yhdE"
                     /locus_tag="ACHLCY_000524"
     CDS             complement(69652..70245)
                     /gene="yhdE"
                     /locus_tag="ACHLCY_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP0367844.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(70254..70742)
                     /gene="mreD"
                     /locus_tag="ACHLCY_000525"
     CDS             complement(70254..70742)
                     /gene="mreD"
                     /locus_tag="ACHLCY_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP0367845.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(70742..71845)
                     /gene="mreC"
                     /locus_tag="ACHLCY_000526"
     CDS             complement(70742..71845)
                     /gene="mreC"
                     /locus_tag="ACHLCY_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP0367846.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(71911..72954)
                     /gene="mreB"
                     /locus_tag="ACHLCY_000527"
     CDS             complement(71911..72954)
                     /gene="mreB"
                     /locus_tag="ACHLCY_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP0367847.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(73259..75199)
                     /gene="csrD"
                     /locus_tag="ACHLCY_000528"
     CDS             complement(73259..75199)
                     /gene="csrD"
                     /locus_tag="ACHLCY_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP0367848.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            75351..76325
                     /gene="yhdH"
                     /locus_tag="ACHLCY_000529"
     CDS             75351..76325
                     /gene="yhdH"
                     /locus_tag="ACHLCY_000529"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP0367849.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            76362..77192
                     /locus_tag="ACHLCY_000530"
     CDS             76362..77192
                     /locus_tag="ACHLCY_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367850.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AISNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTKQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            78058..78528
                     /gene="accB"
                     /locus_tag="ACHLCY_000531"
     CDS             78058..78528
                     /gene="accB"
                     /locus_tag="ACHLCY_000531"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP0367851.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPIMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            78539..79888
                     /gene="accC"
                     /locus_tag="ACHLCY_000532"
     CDS             78539..79888
                     /gene="accC"
                     /locus_tag="ACHLCY_000532"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP0367852.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(79980..81026)
                     /locus_tag="ACHLCY_000533"
     CDS             complement(79980..81026)
                     /locus_tag="ACHLCY_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP0367853.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEMSALKAEIAGYR"
     gene            complement(81023..81985)
                     /locus_tag="ACHLCY_000534"
     CDS             complement(81023..81985)
                     /locus_tag="ACHLCY_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0367854.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(82007..82996)
                     /locus_tag="ACHLCY_000535"
     CDS             complement(82007..82996)
                     /locus_tag="ACHLCY_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0367855.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(82997..84496)
                     /locus_tag="ACHLCY_000536"
     CDS             complement(82997..84496)
                     /locus_tag="ACHLCY_000536"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0367856.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(84557..85447)
                     /locus_tag="ACHLCY_000537"
     CDS             complement(84557..85447)
                     /locus_tag="ACHLCY_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0367857.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(85483..86337)
                     /locus_tag="ACHLCY_000538"
     CDS             complement(85483..86337)
                     /locus_tag="ACHLCY_000538"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFP0367858.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT        21530 a        23112 c        21129 g        20650 t
ORIGIN      
        1 cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc tgtaccattc
       61 tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg attagccata
      121 ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc agcagttaaa
      181 gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg
      241 atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc atacaaagac
      301 atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat acctgtatcc
      361 ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct ttgatgctct
      421 ttcgctgcta aagcaaaata gttgagattg aaaaacggcg tataggtagc aaatcgcgac
      481 gctagtatat atggactgaa cgctttttta ttttttgcat ctttataagc aaattgacaa
      541 gcggaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac tttttttgtc
      601 ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa aaaagcaaag
      661 gcacatagat aagggatata catccccagc attttgacaa cttcatccgg atcactctgg
      721 agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca tgaatagagg
      781 ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag caggcgaagc
      841 ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag caatcgctga
      901 accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat aacacaaaga
      961 tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt catccagaaa
     1021 tccaagttca caaccaatat aaacaattat caacaatgtt caattttata tcagacatgt
     1081 ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt taaatgatgc
     1141 ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac attacgcaga
     1201 acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa gtaaacatat
     1261 ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc agcagaaggg
     1321 aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct acgagtaaag
     1381 caactggacg agatacagat acctgacaac cattcctcag accaggacca aaacgaaaaa
     1441 agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact tgaacccgta
     1501 agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc acctcggcac
     1561 ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag ctggttgagt
     1621 ttgttcggtt ttcaccggtg cactcagatt ttcccattcg ctacctttat tggttttgtt
     1681 gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca gcagcgccgt
     1741 catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg aagcgcctgc
     1801 tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca gaccaacaag
     1861 gccaattgcc acaataagga aaactactaa aagagcttcg tacataatca acctgttcct
     1921 tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta atctttccca
     1981 ctgaagcggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa ttttatcgca
     2041 ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa aaaggcgaca
     2101 atcgccgcct ttttagccag ttatctaacg ctttaaacgg cttttactgc atcggcgatg
     2161 cggtgtgcaa attcagtcac ctgcgcttcg tcttcgcctt ccaccatcac gcgaattaac
     2221 ggttcggtgc cggatttacg cagcaacacg cgtccacggt tgcccagcgc agcttcaacc
     2281 tctgcggtca cggctttaac tgactcatgc tcaagtggat cgccgctacc tgcggtgtaa
     2341 cgtacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc gtgcaggctc
     2401 atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc gtcaccggta
     2461 gtagttttat ccagtaggat cacatgaccg gaattctctg caccgatacg ccagcctttc
     2521 tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa tggaattcct
     2581 aactgtttca gcgccagttc aagccccatg ttgctcatca atgtacccac agcgccacca
     2641 cgtagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg atcgccatcg
     2701 actttattgc cttcatggtc aaccataatc acgcgatcgc catcgccgtc gaaggcaata
     2761 ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc ggtagccccc
     2821 acttcggcat tgatgtttac accgtttggc tcacaaccga tagcgataac gttcgccccc
     2881 agttcgcgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcaca atccaccaca
     2941 atcttcagtt cactgaggct aagttcgttc gggaacgtgg ctttgcaaaa ctcgatatag
     3001 cgacccgcgg catcaacgat acggctggct ttacccagtt ctgccgaatc aacgcagctg
     3061 atctcctttt ccatttccgc ttcgatggcc tcttctaccg catccggcag tttggtgccg
     3121 tcgatagaga agaatttaat gccattatcg tagaacgggt tatgcgatgc agatatcaca
     3181 attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt tggcatcggg
     3241 ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc tgactccagc
     3301 atatagccag aaatacgcgt gtctttacca ataataatct tacgggagcc gtggcgcgcc
     3361 agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat cggcgcatcc
     3421 cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat agcgtttgtt
     3481 ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt ctttgacgtc
     3541 atgaacacga atgatgtgcg cgccttgcat tgcggcaatg accgcacagg ccagactacc
     3601 gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt ttcgtgacat
     3661 acccaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca gtaatgaata
     3721 gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt tctcttttgc
     3781 gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca cttctgcaaa
     3841 gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca tatgcatcag
     3901 acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg aaagggagcg
     3961 gatatcatta ataatgtgag cgccaacttt cgctgactca cggatgactt ctggtttgga
     4021 tgtatcgacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca caggaataac
     4081 acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg actcgccacc
     4141 aacgtcaatg atcgtcgcgc cagcattgat catcagattc gcatgtttca ccgcatctat
     4201 cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga ggatccccat
     4261 tacgtgagga tggctaaggt ccagtgaagt accctgggca aagagtttca tgatgttatc
     4321 cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc ggtacaaata
     4381 cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt gttacccggg
     4441 ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt gtcgccagaa
     4501 ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg acgtgccatc
     4561 aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc tttcatcgca
     4621 tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt acgctcaatc
     4681 agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg tttcgctttc
     4741 gctacgctac ggccgaggaa tacttcacct tcttcttccg cgtacagcag tggacccaat
     4801 ttctcagaga agccccactg ggtcaccatg ttacgtgcca gattggtcgc aactttaata
     4861 tcgttggacg caccggtaga aacatgttcc ggcccataga tgatctcttc tgccagacga
     4921 ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct gattgcgtcg
     4981 ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat cgtcactttg
     5041 tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc ttcgtggtaa
     5101 gccgttgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc acccatcatg
     5161 attttgtctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt gccacgagca
     5221 gcgaacagtg ccgcttcgtt caccaggttc gccaggtcag caccggagaa accaggagta
     5281 ccacgggcaa tgattgccgc gtcgatatcg ggggccaatg gtacgcgacg catgtgaact
     5341 ttcaggatct gctcacgacc gcgaacatct ggcaagccga ccacaacctg acggtcgaaa
     5401 cggccaggac gcagcagggc cgggtcgaga acgtccggac ggttagtcgc ggcgataacg
     5461 atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt cagagtctgt
     5521 tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac ggcgtcgatt
     5581 tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat gtcacgaaca
     5641 cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat agtaaagaac
     5701 ggaactttcg cttcgcctgc aatcgctttc gccagcaacg ttttaccggt acccggagga
     5761 ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg gctcggctcg
     5821 cgcagatact ctaccagttc agcaacttct tcttttgctt cgtcgcagcc cgcaacgtca
     5881 gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt accaaacgac
     5941 atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca gacaccaatc
     6001 agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg ttcttcaggc
     6061 ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt cggatcctga
     6121 accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat ttcacgtccg
     6181 ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt agagtaatcc
     6241 accttacggc cattagactc gctgggccca aagctctgga atactgacat cagcacaacg
     6301 gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt aacctcttat
     6361 tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc aaactttcgt
     6421 ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc ccgcgaacgt
     6481 gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg aatttccctt
     6541 agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc acctggcgct
     6601 aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg ggggatatcc
     6661 accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg gactttgctg
     6721 tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc gccctgaaga
     6781 aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat gcggcctttg
     6841 ccgccaattt ggttgaccac atattgtgac caaccacccg gagcagcacc aaggtcgaca
     6901 accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag tttaaaccag
     6961 gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct aaagtgttcc
     7021 tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact ttcccatggg
     7081 gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga tatatgggag
     7141 atggcggtag aatgacccgt tttcaatccc aacgtaagca aaaaatacga tgaatctgag
     7201 tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag ttgttctgct
     7261 tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt tagagcacca
     7321 tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa actaaaacct tgatcgtgga
     7381 agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa cgctggtgct
     7441 ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc ctaaagttac
     7501 acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa tgccacgctc
     7561 tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata catcaacatc
     7621 ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt ctacttcgcc
     7681 gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc cacgagcaat
     7741 aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc caacgatgcg
     7801 ataagtctgt tctttgtcag aatccagatt cagcaccgtt acggtagcac cgaaaataac
     7861 gcgcccattg ttgggcattt tggtgacatc aatcacctgc gcgttcgaca gcttggcttc
     7921 gatgtcttta atacggcctt cgcagaaacc ctgctgttca cgagctgcgt ggtattcggc
     7981 gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga tttcaggacg
     8041 gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc gtaaggtcat
     8101 cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag tcttcaccca
     8161 ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca aaaaaatacc
     8221 gacccgggta caagtcccag gtcagctaca attcacattt tgatagtcat tttaccctga
     8281 agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg acggctcgat
     8341 tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat tgaccagctg
     8401 tatagcgttc agtgttcagg ccgcgaatgt tgatgagtac gttactcaac tccccgctgg
     8461 tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta ttgattacca
     8521 cagtcagcag atggcgctgc ccgccagtac gcagaaagtg attactgcgc tggcggcgtt
     8581 gattcaactc ggccccgatt ttcgtttcac cacgacgctt gaaaccaaag gcaatgtgga
     8641 aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga cgttaaaacg
     8701 tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc aaatcgatgg
     8761 caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg gctggccatg
     8821 gaatgacatg acacaatgct ttagcgctcc gcctgccgct gccatagttg accgcaactg
     8881 tttctccgtc tcgctctaca gtgccccaaa acctggtgat atggctttta tacgcgtggc
     8941 atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgag gttctgccga
     9001 agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta cgctgacggg
     9061 atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg atggagccag
     9121 ctatgccggg gcaattctga aagatgagtt aaaacaggca ggtatcacct ggagcggaac
     9181 actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta aacagtcggc
     9241 cccgttgcac gatctgctta agattatgct gaaaaagtcg gacaacatga tcgccgatac
     9301 ggttttccgc atgataggcc atgcgcgctt caatgtgcct ggaacatggc gggccgggtc
     9361 ggacgccgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa acaccattat
     9421 tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca tgatgcaggt
     9481 gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc tgccgcttgc
     9541 gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg tggatggaaa
     9601 agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat tcattaccac
     9661 agcgagcggg caacgaatgg cgtttgtaca atatctttct ggctatgcag tagaaccggc
     9721 ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt ataaagatat
     9781 ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggtttt gctgattaac
     9841 gcttgtaaat gaactcaacg ccttcttcgt cgtcttcgtc ccagtcatca tcccagtcct
     9901 catcatcctc ttcagcaatc tcttcaagct gctggcgatg ataatcatcc cacatgaatt
     9961 cgactttctc tggctgtttc gcttcttcag cctgcacgac cgggttttca atgataaagg
    10021 tcatcacatc ccagcagaga tctttcacgc ccagtccact cgccgcagag atcagataat
    10081 atttatcttc ccagcccagc gcttcagcaa tcgctttcgc tttctcttca gcttctgcct
    10141 tatccagcag atcgatctta ttgaacacta accaacgcgg tttcgccgcc agatcctggc
    10201 tgtatttttc cagctcgctg ataataatac gcgcgttttc aaccggatcg gtgccgtcaa
    10261 tcggatcgat atcgatgagg tgcaacagga cgcggcaacg ttccaggtgc ttcaggaagc
    10321 ggatacccag acctgcgcct tccgcagcgc cttcaatcag tcctggaata tcggcaacaa
    10381 cgaagctctt ttcgttgtcc attcgtacca cacccagact tggcaccaga gtggtaaacg
    10441 gataatccgc cactttcggt ttagccgccg ataccgcgcg aataaaggtc gatttacccg
    10501 cgtttggcat ccccaacata ccgacgtcag ccagcagcat cagctccagc agcagctcac
    10561 gcttatcacc cggcgtaccg ttggttttct gccgtggtgt acggttaacg gacgatttga
    10621 aacgggtatt gcccagaccg tgccagccgc ccttagcaac cagcagacgc tgaccgtgtt
    10681 tagtcatatc gcccatggtt tcaccagtac cttggtcgat tatacgcgta cctaccggca
    10741 ctttaatcgt cacgtcttta ccgcgcttac cggtacaatc gcggcttgcg ccgttctgac
    10801 cgcgctctgc acggaaagat ttttcaaaac gataatcgat aagcgtgttc aggttttcgt
    10861 cggcttccat ccatacgtca ccaccatcac caccgtcgcc gccatccggg ccgcctttcg
    10921 gaatatactt ttcgcggcgg aagctcacgc aaccattacc gccatcacct gcaacgacca
    10981 gaatcgatgc ttcatcaaca aacttcattt tattctccgt aaatcattcg cctgcgcggg
    11041 gttgcgatac caccgttgta tgcttacgta atccgcccca aatacgatga ccaatggcgg
    11101 aatacatcgc gcccgcaacc acgacaaacg caccgagata acctaaaagg tttaacatcg
    11161 gtctggcgaa gaaatcgggc caggccagtg ataaaagatc tgaaaaaaac agcgtaaaca
    11221 gtggggtgag cgtgatgatc gcgctcacct gcgctgcctg ccagcgagcc atcgcttccg
    11281 ccagggcgcc atatcctacc aatgtattca gtccgcaaaa aattaaacat gcgagctgcc
    11341 agtggctaag ctgcgctatc actccaggct ttgccagagg gaagagcgca attgtacata
    11401 aagtgtacag taaaaacagg atctgcggtg aggccagccg acgcaataaa accttttgcg
    11461 ccacgccata actcacccaa accgtcgccg caccgacccc aaagataact ccccaggtgt
    11521 aatcggtgag ctttgtaaat atctcgacca gactggtgtt aaaaaacatc accaggccgc
    11581 tcaggagcat caatgcccct acaacctgag tgctgcgcat tttctctttc aggataaata
    11641 cgctggcaac catcatgcca actggcgaga gttgcccaat cacctgcgaa gcggtcggac
    11701 tcaggtattg caaggatgag ctgaacagga tgaagttccc aaacagcccg gcggtcgcca
    11761 ctgccaacaa aatcaaccag cgtggcttac gaaacacgcg taatggcggc aacctcttct
    11821 tcaccgcaag aatggcacca aggccaatac tcgccatcaa gaaacggtaa aacacgattg
    11881 tcggaggttc catcacctcc agcacctgct tcattgcgat tggcaacgcc ccccagcaaa
    11941 ttgctgtggt gagcgccaaa agaatgccaa tgcctgcctg ctgcttcata ccgtattccc
    12001 tgcaaagcgc atttgcggat taacgtcgca ccatgcgcat ttttaccggt tatcgaatgt
    12061 aaaaagcccc gcaacgtgtt gcggggcttt catccgttac cgggacgcga aaaacttatt
    12121 cagcttcgat gctgataaat ttacggtttt tcgggccttt aacttcgaat ttcactttac
    12181 cgtctgcttt agcaaacaga gtgtggtcac gaccgcaacc tacgttagcg ccagcgtgga
    12241 atttggtacc acgttgacga acgatgatgc tacccgccag aacggattcg ccaccgaaac
    12301 gcttaacgcc caggcgttta gcttctgaat cgcgaccgtt acgtgtggag ccgccagcct
    12361 ttttatgtgc catttgaaat ctctcctcag gtcttaggcg ctgatgccag taattttcac
    12421 atcagtgaac cactgacgat ggccctgctg cttacgatag tgtttacgac gacgaaactt
    12481 aacgatttta actttctcgc cacgaccgtg agcaacaact tcagctttga ttacgccgcc
    12541 atcaacgaaa ggaacgccga ttttgacttc ttcaccgttt gcgatcatca gcacttcagc
    12601 gaactcaaca gtttcgccag ttgcgatgtc cagcttttcc aggcgaacgg tctgaccttc
    12661 gcttactcgg tgttgtttac caccactttg gaaaaccgcg tacatataaa actccgcttc
    12721 cgcgcacacc ttttcaatga ttcagagtgc gctataaata ttcacaatag ggcgcgaata
    12781 ttacgcaaaa cgcacgcctt tgacaagtgc tacagtcaat acacgaagaa aaaaaacaca
    12841 acttgtacgg taacgtttat ctgtgccatt ttttcagtac aatcactcta tattcctaac
    12901 cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt
    12961 gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg
    13021 gcaatccttg agcagcttaa ttccgacgtc cagctgatca atcagttagg ctattacatc
    13081 gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt
    13141 ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat ccacacggcg
    13201 actctgctgc acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc
    13261 aacgctgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg
    13321 ttccagatga tgaccagcct cggttcgctc aaagtgctgg aagtcatgtc agaagccgta
    13381 aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact
    13441 gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg
    13501 cagtgttccg ggattctggc tggctgtacg ccggaagagg agaaaggcct gcaggattat
    13561 gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat
    13621 ggcgaacagt taggtaaaaa tgtcggcgac gatctgaacg aaggtaaacc gacgctgccg
    13681 ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg taccgccatc
    13741 gaacagggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga
    13801 tctcttgaat ggacgcgtca gcgtgccgag gaagaagcag acaaagccat cgcagcgtta
    13861 caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt
    13921 caacgcgatc gttaatcccc tcccctcatc ccgcgcagcg cgcgggatgg ttccagtaaa
    13981 ttccataaaa acacttactc agctctataa cacctgaaaa acaggctgtg catctttata
    14041 tattctgaat attcacactc cttacaggaa ctttttagag caatcggcca tcaggagtat
    14101 agtgatgctc gacagaagaa gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa
    14161 gatggaaagt aatttcattg actggcatcc cgctgacatc attgcgggtt tgcgcaagaa
    14221 gggaacgtca atggcggcgg agtctcgcag aaatggtttg agttcctcaa cgctggcgaa
    14281 tgcattatcg cgcccatggc cgaaaggaga gatgattatt gcgaaagccc tgggaactga
    14341 cccctgggtt atctggccat cacgctacca tgatccgcag acccatgagt ttatcgacag
    14401 aacgcagttg atgcgtagct acactaaacc gaaaaaatga gtggtctggc ggtagccccg
    14461 cgaacggggc tgccagctct cagacgatta ctcgcctttc acacgctcaa tatttgcacc
    14521 taaagcgcgc agtttgtctt caatgcgttc gtagccacga tcgatgtgat aaatacgatc
    14581 aaccaccgtc gtcccttccg caatacagcc agccagcacc aggcttgctg atgcacgcag
    14641 atcggttgcc ataacctgtg cgccagaaag tttttcaaca ccgtgacaaa taacggtatt
    14701 gctttcgatt tcggcatgcg cgcccatacg gctaagctcc ggcacatgca taaagcggtt
    14761 ttcaaagacc gtttcggtga tgaatccggt cccttctgcc accaggttca acagcgtgaa
    14821 ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt
    14881 cggacgtttg ccatgcatat ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc
    14941 agcgtcacgc agtttcgcca gcacggcgtc gagagtatct ggctgcgcgt tacggcagat
    15001 aattttgccg cgagaaatcg ccgccgccac caggaaagta ccggtttcga tacgatccgg
    15061 tagcacgcga tagacaccgc cgcctaaacg ttccacacct tcgatgacga tacgatcggt
    15121 gccctgaccg ctaattttcg cacccagcgt aatcaggaag ttcgcggtat cgacgatttc
    15181 cggttcacgc gctgcgtttt caataatcgt ggtaccttcc gccagggttg cagcacacat
    15241 gatggtcacc gttgcgccaa cgctgacttt atccatcacg atatgtgcgc ctttcagacg
    15301 accatcgacg gaagctttaa cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc
    15361 gaggccagaa atgtgtagat caaccggacg cgcaccgatc gtacaaccgc caggcagtga
    15421 aacttgcccc tgaccaaagc gcgctaccag cggccccagt gcccagatag aagcacgcat
    15481 ggttttaacc agatcgtaag gtgcgcagaa tacattaacg tcgcgggcat caatatgcac
    15541 agaaccatta cgttctactt tcgcacccag ctggcttagc agcttcattg atgtatcgac
    15601 gtctttcagt ttcgggacgt tctggatctc taccggttct tccgccagta gagcggcaaa
    15661 aaggataggc agagcagcat ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt
    15721 tggcccctga acacgaaatt tatccattta gtttgttctc agttaacaat tcatatccgc
    15781 taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc
    15841 aggggtatac gctttgatcg acacagcatg aatgcggtta tccgcaatat attccatcag
    15901 cgggccatag accgtctgct gttttttaac ccgactcatg ccgtcaaaca actcacccac
    15961 ggcaataacc tgaaagtggc tgccatcgcc ggaaacgtgg acttcctgga gggagagagc
    16021 gttcatcaac acgctctgaa tttcattatt ttccatggga tcttcaatca tcagttaata
    16081 aaccagcgaa acatcttaga gcaaagttgc gctggcataa ataagcaaaa agcctcgctg
    16141 ataaatcaga caaggctcga cttgcaggca ggtttgccgg acaggcggtt aacgccatat
    16201 ccggcctgaa aaaatttaac gaggcagaac atcagcaggc aaattataca atttcgcgag
    16261 ggtatacact ttgtcgttta ccccctgaag cgtcacgttg ttgccctgct ttttcgccag
    16321 atcgataaga tggagcagca gtgccagtcc acccgtatcc acgcgggaca cacggctaag
    16381 atcgatgcag gtaatcccct tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac
    16441 gtcctgatcc agctctccgg ataacgccag cgtgtcaccc gtctgcatcc agctcagtga
    16501 ctcgctcatt attttttctc ttccagagtg attttctgtt gagaaatcga tttcagttgc
    16561 gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc cccactcgtt ttgtttggtg
    16621 gtgatcatac tgacgccttc agcaatcatg tcgtaagcct gccagtttcc cgtctgggag
    16681 tttttacgcc actggaagtc cagacgcacc ggcggacggc cattcgggtc aataatggta
    16741 acgcgaatag gcacaatggt tttatcgccc agcggctgtt ctggcgcaat ctgataggtt
    16801 tgaccgtgat acatcgccag cgcctgaccg taagcctgct tcaggtactc acggaaagcg
    16861 gcaaagtagg cttcacgttg agcaggggtc gcactcttgt aatactggcc cagcaccagc
    16921 gcaccggcgt atttcacctg tacgtatggc agcagttcct gatcaacaat ggtacgcaga
    16981 taatccgggt tggcccgaat ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc
    17041 gccgcctcgt ccatcagctt atacggattg gtctggtctg ccgcggttgc cgcactcaga
    17101 ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa acatgcgtcg gttctcctga
    17161 aattatttcg ttgtacccac aggttcagtg gtttcattat tacctggcgc agcagctggc
    17221 gcatcgccac tattcttatt gtcatcgcct ttactaccgt aaaggaactg accaatgaga
    17281 tcttccagca ccatcgcaga cttagtgtcc tgaattgtat cgccatcctt caggatagca
    17341 gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca gatattgttc ccccagcagg
    17401 ccggaagtac gaatgctcag cgaactggta tctggaatgt ggttataacg ttgttcaatt
    17461 tccagcgtta cgcgtggcag ataggttttc gggtccagcg taatatccgc cacccggccc
    17521 acaacaacgc caccaatact gaccggagag cgcgctttca ggccgccaat gttatcgaac
    17581 gtcgcataaa gcgtgtaggt cggttcagta cgtatggacg tcacgttcgc cgccttcagg
    17641 caaacaaaca gcgccgccag cagtgctgct aataaaaaga tacccaccca aatttcattt
    17701 tttttcgttt gcatgaactc aattcccaaa catcaatgcg gtcagcacaa aatccagccc
    17761 cagaacagcc agagacgagt ggacaacggt gcgagtggtt gcccggctaa tcccggcaga
    17821 cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac
    17881 cacgctctta atcagacagt tgaccagatc catacgccag tcgacggcat tttgcattgc
    17941 cgaccagaag aacccgctat caatgccttt ccaactgacg ccgaccagag atccgcccca
    18001 gatccccacg gcgacgaaaa taaccgtcaa cagtggtaat gaaataaccc cagcccagaa
    18061 acggggagaa ataacccgac gcagcggatc caccgccatc atctccatac tggagagttg
    18121 ctctgtagcg cgcatcaggc cgatttctgc ggttagcgcc gaaccagcac gcccggcaaa
    18181 caacaacgcg gcaaccaccg gccccagttc acgcagtagc gataacgcca ccagcatacc
    18241 cagactggtt tccgcactat aagtggtcag aaccagataa ccttgcagcc ccaacaccat
    18301 tccgatgaac acgccagaaa ccacaataat cagcatcgac aggacgccga cattatagag
    18361 ctggcgcacc agcagcggcg catgtttgcg aaattccggt ttgccgacca gcgcattgaa
    18421 taacattaac ccggcccgcc cgaacgttct cagggtttta atccctttat gtccgagcga
    18481 cgccagcgca tttaacagca tgagtggctt aactccctgg taaaagatca gcgtgataat
    18541 cgccggcagg atagcggaac ggaacaggcc cgtcagctat cccgtccaga aactgacgta
    18601 cgcgcggatc aggattcgcc tgcaacgcct gggcactgcc atgagcgaca atttttttgt
    18661 ccgccaggat ccaggcgtga tccgcaatac ttaacacttc cggcacatcg tgagaaacca
    18721 ccacacaagt cacgcccagc gcgctgttca gctcagaaat cagcttcacc agtacgccca
    18781 tggtgatggg atcttgccca acaaaaggtt catcaaacat gatgagatcc ggctccagcg
    18841 caatcgcacg cgccagcgct gcacgccgcg ccatcccacc agaaagttca gaaggcatta
    18901 gcttagccgc tccacgcagc cccacggcct ccagcttcat catcactgta ctatgcaaca
    18961 atggcgcggg aagttgggta tgttcgcgca gtggataggc gacgttgtca aatacgttca
    19021 tatcagtgaa caacgccccg gactgaaata acatgctcat ccgtttgcgc actgtataca
    19081 ggcgcgaacg agacatcgcc ggaatattct caccatcgaa aaggatctca ccatgatctg
    19141 gtgcgatttg cccgccaatc agacggagta gcgtcgtttt accgatgccc gatggcccca
    19201 tgattgccgt gatcttccct cgcggcacgg tcagggaaat attatcgaag atgcagcgat
    19261 tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt cgccacagac tgctccataa
    19321 ttcacccttc gtcttgcgtt gattttctaa gcatggcgct caatttaacc ttgaacccaa
    19381 catatttaca gaatattacc cgccgtggtt agcgaaagct ggcatttgtt ttacttttta
    19441 gccgcataaa gtcaaaatta agaatccgtt acggctttct gaaaatcttc agcggaccgg
    19501 cgagtatacc tgaagaaagg atgttagatg cttttagcta cggcactgtt aattgttggt
    19561 ttacttttgg tcgtttacag tgccgaccgc ctggtttttg ccgcgtctat tctttgccga
    19621 acctttggca tcccgccgct gatcatcggc atgacggtgg tcagtattgg tacatcgtta
    19681 ccagaagtca tcgtctcgct tgccgcgtct ctgcacgaac aacgcgattt agccgttggt
    19741 acagccctcg gctcaaacat tatcaatata ttgctgatcc tcggtctggc tgcgctggtt
    19801 cgtcctttta ccgtccattc tgatgttcta cgccgtgaat tacccttaat gttgttggtc
    19861 agcgtggtgg ccggttccgt actctatgac ggacaactta gtcgcagcga tggtatcttt
    19921 ctcctctttc tggctgtgct atggctgctg ttcattgtta aacttgcacg tcaggctgaa
    19981 cgtcagggga ctgacagcct gaccagagag cagcttgcag agctgccgcg tgacggcgga
    20041 ttgcccgtcg cgtttttatg gctcggcatt gcgcttatca tcatgccagt ggccacgcgg
    20101 atggtggttg ataacgccac ggtgctggcg aattactttg ccatcagcga gttgacgatg
    20161 ggtctgacgg caattgctat cggaaccagc ctgccggaac tggcaaccgc aatagcgggg
    20221 gttcgcaaag gtgaaaacga cattgctgtc ggaaatatca ttggcgcaaa catttttaat
    20281 attgtcatcg tgttgggttt acccgcgctg ataacgccag gagagattga tccactggcg
    20341 tacagtcgtg actacagcgt gatgttgctg gtgagcatta tttttgcgtt gctgtgctgg
    20401 cggcgctccc cacaaccgag ccgtggtgta ggggtattat taactggcgg atttatcgta
    20461 tggctggcga tgttgtactg gttatcgcca atactcgttg aataactgga aacgcattat
    20521 gtcgcacgta gagttacaac cgggttttga ctttcagcaa gcaggtaaag aagtcctggc
    20581 gattgaacgt gaatgcctgg cggagcttga tcaatacatc aatcagaatt tcacgcttgc
    20641 ctgtgaaaag atgttctggt gtaaagggaa agttgtcgtc atggggatgg ggaaatcggg
    20701 gcacatcggg cgcaaaatgg ccgcaacgtt tgccagcacc ggtacacctt catttttcgt
    20761 ccatcctggt gaagccgcgc atggtgattt aggcatggtc accccacagg atgtggtgat
    20821 tgctatctct aactcaggtg aatccagcga aatcacggcc ttaattccag tgcttaagcg
    20881 tcttcacgta ccgttaatct gcatcaccgg tcgcccggag agcagcatgg cgcgcgccgc
    20941 agatgtgcat ctgtgtgtta aagtagcgaa agaagcctgt ccgttagggc tggcaccgac
    21001 cagcagcacc accgccacgc tggttatggg cgatgccctc gctgtcgcgc tgttaaaagc
    21061 acgcggcttt actgctgaag attttgcgct ctcacaccca ggcggcgcac tgggtcgtaa
    21121 acttctgctg cgcgtaaacg atattatgca tacgggcgat gagatcccgc atgttaagaa
    21181 aacggccagt ctgcgtgacg cattgctgga agttacccgc aaaaatcttg gtatgactgt
    21241 catttgcgat gacaatatga tgattgaagg catctttacc gacggtgatt tacgccgtgt
    21301 cttcgatatg ggcgtggatg ttcgtcagtt aagtattgcc gatgtgatga cgccgggggg
    21361 aatacgtgtg cgccctggca ttctggccgt tgaggcactg aacttaatgc agtcccgcca
    21421 tatcacctcc gtgatggttg ccgatggcga ccatttactc ggtgtgttac atatgcatga
    21481 tttactgcgt gcaggcgtag tgtaaagatt caaggataaa caacaatgag caaagcaggt
    21541 gcgtcgcttg cgacctgtta cggccctgtc agcgccgacg ttatggcaaa agcagagaac
    21601 attcgtctgc tgatcctcga cgttgatggc gtactgtcag atggcctgat ttatatgggc
    21661 aataacggcg aagagctgaa agcgttcaat gttcgtgacg gttatggcat tcgttgtgcg
    21721 ctcacctctg atattgaagt cgctatcatt accgggcgaa aggctaaact ggtagaagat
    21781 cgttgtgcca cattggggat cactcacttg tatcaggggc agtcaaacaa actgatcgcc
    21841 tttagcgatt tgctggaaaa actggcgatt gccccggaaa atgtggctta tgtcggcgat
    21901 gatctcatcg actggccggt aatggaaaaa gtgggtttaa gcgtcgccgt ggccgatgcg
    21961 catccactgt tgatcccgcg cgccgattac gtgacgcgca ttgctggcgg tcgtggcgca
    22021 gtgcgcgaag tttgcgactt attactcctg gcgcagggca aactggatga agccaaaggg
    22081 caatcgatat gagtaaagcc agacgttggg ttatcattgt gctatcactg gcggttctgg
    22141 tgatgatcgg cattaatatg gccgaaaaag acgataccgc ccaggtggtc gtcaacaaca
    22201 atgatcccac ctataaaagc gagcatacgg acacgctcgt ctataaccca gaaggggcac
    22261 taagctatcg attgattgct caacacgttg aatattattc cgatcaggcc gtttcgtggt
    22321 ttacgcagcc ggtacttacc acgtttgata aggataaaat cccgacatgg tccgtaaaag
    22381 cagataaagc caagctgacc aatgaccgga tgctctattt atatggacac gttgaagtca
    22441 acgcactcgt gccggactct caacttcgca gaatcacgac ggataacgcg cagatcaatc
    22501 tggtgacgca ggatgttacc tctgaagacc tcgtcacgtt atacggaaca acatttaact
    22561 ccagcggtct gaaaatgcgc ggcaacttac gcagcaagaa cgccgagctg attgaaaagg
    22621 ttagaacatc ctatgaaatt caaaacaaac aaactcagcc ttaatcttgt gcttgccagc
    22681 tcacttctgg ccgccagcat tccggcattt gccgtaaccg gagacactga tcagccgatc
    22741 cacattgaat cggaccagca atctcttgat atgcaaggca acgtggttac ctttaccggt
    22801 aatgtcatcg tcacccaggg caccatcaaa attaatgccg acaaagtggt tgttacccgt
    22861 ccgggcggcg aacaaggtaa agaagtgatt gacggctacg gtaaaccggc aacgttctac
    22921 cagatgcagg acaacggtaa accggttgaa ggtcacgctt cccagatgca ctacgaactg
    22981 gcaaaagatt ttgtcgttct gacgggtaat gcttatctgc agcaggttga tagcaacatt
    23041 aagggcgata agatcactta cctggtaaaa gagcagaaaa tgcaggcttt cagcgacaaa
    23101 ggcaagcgcg taaccaccgt tctggtgccg tcgcagctgc aggacaaaaa caacaaaggc
    23161 cagaccccgg cacagaagaa gggtaattaa ttcgttatgg caacattaac tgcaaagaac
    23221 cttgcaaaag cctataaagg ccgtcgcgtg gtagaagacg tcagcctgac cgtcaactcc
    23281 ggggaaattg tcggtctgct ggggccaaac ggtgccggta agaccaccac tttctatatg
    23341 gttgtaggca ttgtgccgcg cgatgcgggc aacatcatta ttgatgatga cgatatcagt
    23401 ctgctgcctc tgcatgcacg cgcgcgccgc ggtatcggct atctgccaca ggaagcctcc
    23461 attttccgtc gcctcagcgt ttacgataac ctgatggcgg tactgcaaat tcgtgacgac
    23521 ttgtctgctg aacaacgtga agaccgcgcg aacgagctga tggaagagtt tcacattgag
    23581 cacctgcgtg acagcatggg gcagtcactc tccgggggtg aacgtcgccg tgtagaaatt
    23641 gcccgcgcac tggctgcgaa tccgaaattt attctgctcg acgaaccgtt tgccggtgtt
    23701 gacccgatct cggttatcga cattaaacgc atcattgagc acctgcgcga cagcggcctg
    23761 ggcgtgctga tcactgacca caacgtgcgt gaaacactgg cggtttgtga acgcgcttat
    23821 atcgtcagtc aggggcattt gatcgcccac ggcacgccta cagaaatctt acaagacgaa
    23881 cacgttaagc gtgtatacct tggggaagac ttcagactct gatagggtag aagtttgcga
    23941 cgttttagca ggagagtacg attctgaaca tgaagcaagg tttgcaactc aggcttagcc
    24001 aacaactggc gatgacgcca cagctccaac aggcaattcg tctgttgcag ttgtcgacgc
    24061 tggaacttca gcaggagcta cagcaggcgc tggagagtaa tccgctgctt gagcaaatcg
    24121 acactcatga agaaatcgac acccgcgaaa cgcaagacag tgaaacgctg gacaccgccg
    24181 acgcgctcga acaaaaagag atgccggaag agctgccgct cgatgccagt tgggacacca
    24241 tttacaccgc tggtacacca tccggcacca gcggtgacta cattgacgat gagctgccgg
    24301 tctatcaggg cgaaacgacg cagaccttgc aggattacct gatgtggcag gtcgagctga
    24361 caccgttttc cgacactgac cgcgctattg ctacctctat cgtcgatgcc gttgatgaca
    24421 ccggttatct gactgtcccg ctggaagata ttctcgaaag tatgggcgat gaagagatcg
    24481 acatcgacga ggttgaagcc gtccttaagc ggatccaacg gtttgatccg gtcggtgtag
    24541 cggcaaaaga tctgcgtgac tgcctgctaa tccaactctc ccaattcgat aaaaccacgc
    24601 cgtggctgga agaggccaga ttgatcatta gcgatcatct cgatctgtta gccaatcacg
    24661 acttccgcac tttaatgcgc gtcacgcgtc tgaaagaaga tgtgctgaaa gaagccgtca
    24721 atctgatcca gtcgctcgat ccgcgccccg ggcagtcgat ccagactggc gaacctgagt
    24781 atgtcattcc agatgtgctg gtgcgtaagc ataacggtca ctggacagta gaactcaaca
    24841 gtgacagcat tccgcgtctg caaatcaacc agcactacgc ctcgatgtgc aataacgctc
    24901 gcaatgatgg tgacagccag tttatccgca gcaatctgca ggatgccaaa tggttgatca
    24961 agagtctgga aagccgtaac gatacgctac tgcgcgtgag tcgctgtatc gttgaacagc
    25021 agcaagcctt ctttgagcaa ggcgaagaat atatgaaacc gatggtactg gccgatatcg
    25081 cccaggctgt cgaaatgcat gaatcgacga tatctcgcgt gaccacgcaa aaatacctgc
    25141 atagtccacg aggcattttt gaactgaagt atttcttttc cagtcacgtc aataccgagg
    25201 gcggcggcga agcttcctcc acggcgattc gtgcgctggt gaagaaatta atcgcggcgg
    25261 aaaacccagc gaaaccgttg agcgacagca agttaacctc tttgctgtcg gaacaaggta
    25321 tcatggtggc acgccgcact gttgcgaagt accgagagtc tttatccatt ccgccgtcaa
    25381 accagcgtaa acagctcgtt tgacccaacc gataaggaag acactatgca gctcaacatt
    25441 accggaaata acgtcgagat caccgaggca ctgcgcgaat ttgttacagc caaatttgcc
    25501 aaacttgagc aatattttga ccgaatcaac caggtctatg ttgttctgaa agtggagaaa
    25561 gtcacccaca cctcagatgc aacactgcat gtaaacggtg gtgaaattca tgccagcgcg
    25621 gaaggtcagg acatgtacgc tgccattgat ggcttaattg ataagctggc acgccagttg
    25681 accaaacata aagataaact gaaacaacac taattgtccg ggcaattagc atgtgcatgg
    25741 cggcctgttg tgcggcacaa cgggccattt gtacggttaa tgctccgagc ctgttccact
    25801 gtttgagtgg gcaggttctt aggtgaaatt atgacaaata atgatacaac tctacagctt
    25861 agcagtgttc ttaacaggga atgtacgcga agccgcgtcc actgtcagag caaaaaacgc
    25921 gccctggaaa tcatcagcga gctggcggcg aaacaactta gcctgccgcc tcaggtggtt
    25981 tttgaagcta tcctgacgcg ggaaaaaatg ggcagtaccg gtatcggcaa tggtattgcc
    26041 attccgcatg gcaaactgga agaagatact ctgcgcgccg ttggcgtttt cgttcagctc
    26101 gaaacgccta tagctttcga tgccatcgac aaccaaccgg tggacttact ttttgccctg
    26161 ctggtgccgg cagaccaaac taaaacgcac ctgcatactc tgtcgctggt ggcgaaacgt
    26221 ctggcggaca aaaccatctg ccgccgttta cgcgcagccc agagcgatga agagctgtat
    26281 caaatcatta cggataccga aggtactccg gatgaagcgt agttattcgg taatgtctct
    26341 tttagacgtt gtgaggagaa acagtacatg gtactgatga tcgtcagcgg acgttcaggt
    26401 tcaggtaaat ctgtcgccct gcgtgcgctg gaagatatgg gtttttactg cgtggataac
    26461 cttcccgtag tgttgttacc cgatctggct cgaactctgg ccgatcgcga gatttctgcc
    26521 gccgtcagca ttgatgttcg taatatgccg gagtcaccag aaatattcga acaggcgatg
    26581 agtaacctgc ctgacgcttt ctcaccgcaa ctactgttcc tggatgccga ccgtaatacc
    26641 ttaattcgtc gttacagtga cacgcgccga ctgcatccgc tttccagcaa aaacctgtcg
    26701 ctggaaagtg ctatcgacaa agaaagcgat ttgctggagc ctctgcgttc gcgagcggat
    26761 ctgattgtcg atacctcaga aatgtccgtt cacgagctgg ctgaaatgct gcgtacccgt
    26821 ctgctgggta aacgtgaacg cgaactgacc atggtctttg agtctttcgg cttcaaacac
    26881 ggtatcccta tcgatgcaga ttacgtcttt gacgtgcgct tcttgccgaa cccgcactgg
    26941 gatccgaaac tgcgtccaat gacaggtctt gataaacctg tcgccgcgtt cctcgaccgc
    27001 cacacagaag tacacaattt tatctaccag acgcgaagct atcttgaact atggttacct
    27061 atgctggaaa ccaacaaccg tagctacttg acggtcgcca ttggttgtac cggcggaaag
    27121 caccgttcgg tgtatattgc agagcaactg gcagactact tccgctcgcg cggtaaaaac
    27181 gtccagtcac gccatcgtac gctggaaaaa cgtaaaccat gaccgtcaag caaactgttg
    27241 aaatcacaaa caagctgggc atgcatgccc ggcctgcaat gaagctgttt gaattaatgc
    27301 agggttttga cgctgaagtg ctgttacgta atgacgaagg caccgaggcc gaagccaaca
    27361 gcgttattgc gctgctgatg ttggattctg ccaaaggacg gcagattgaa gttgaagcga
    27421 ccggcccaca ggaagaggaa gcactggccg ccgttatcgc cctctttaac tctggttttg
    27481 atgaagatta atcttcatca ctttgacata caactccctt caaactcccc ccgacaataa
    27541 gaaaatcacg tactgaaatc gttctcaatc aacgtcattt gtacattttg tgcgcttttc
    27601 actttcagaa gaaccttaag aaaaccttaa gaggcattgt ttaggttttg tttaagttaa
    27661 tcgaccatac tggagatcgt cagaaaatat ttccaggaga tggcatgatt cgcttatctg
    27721 aacaaagtcc cctgggcacc ggaagacatc ggaaatgtta tgcgcatccg gaagatgccc
    27781 aacgctgtat caagattgtc taccatcgtg gcgatggtgg cgataaagag atccgccgtg
    27841 agttaaagta ctacgcgcat cttggtcgcc ggttaaaaga ctggagtgga ataccgcgct
    27901 atcacggtac cgttgagacg gattgcggaa ccggttatgt ctacgatgtg attgcagatt
    27961 ttgacggcaa accctccatt acgctgaccg aatttgctga acaatgccgt tacgaagaag
    28021 acattgcaca actgcgccag ctactgaaac agctgaagcg ttatttgcag gataaccgta
    28081 tcgtgacgat gtcgctgaag ccgcagaata tcctctgtca ccgcatcagt gagtcagagg
    28141 ttatcccggt cgtctgtgac aacattggtg aaagcacatt gattccactg gcgacctggt
    28201 cgaagtggtg ttgtctgcgt aagcaagagc ggttatggaa acgatttatc gcccaaccgg
    28261 cgctggctat tgccctgcag aaagatctgc aacctcgtga gagcaaaacg ctggccctca
    28321 cttcgcgcga agcttaatcc agctggtgtt gttgcataaa cggctcaccg cctaactgat
    28381 acatctgccg taaaatccac gcctgacggc tacgcacgta gcccgatggc gaggagactt
    28441 tgaaacgaag tggattaggt aatacagctg ccagtaatgc agcttccgac cgggtaagtt
    28501 tgctcgcggg tttgtggaaa taacgttgtg ccgcagcttc gacgccaaac acaccgtcgc
    28561 caaattcggc gatattcagg taaaccgtca ggatacgctt tttgctccag actgtttcta
    28621 tccccagcgt taatccagct tccagccctt ttcgtaccca gctacgccca tcccataaaa
    28681 agagattttt ggccgtctgc tgagaaatcg ttgaagcacc gcgaatacgg ttttcattgc
    28741 gctcgttgtg cgccagggct ttctcaatgg aagcgacatc aaagccccag tgctcaggaa
    28801 atttctgatc ttctgcggca atcaccgcca gtcccatcca cggcgagatt tgatccatac
    28861 tgacccagtc agaatgtgcc acgtaacgaa aattgccatg cagccaggcg ctgacctgtc
    28921 gctcgaccat taccgctgag aagggaacag gcgcaacact aaacaacgcg atgcccccgc
    28981 cccagaaaac ggcgaggaca accattaacc gcaaaagaaa acggcgaacg aaactaaaca
    29041 ccgttaagcg gcttttactc attcagccag aaccagcacg cgggaaacca gcttatcaat
    29101 gccgctcgcc gcttctgcaa tgttctgcgc cagcatatat gctggggtgg tgacaatctt
    29161 attgtcttca tcaaccacga tatcatcgac aggacagggc acatgctccg cgcccatctc
    29221 ttccagcact tctgcggtat cgatatcagt accgatggtc aaacgcagcg ggaaatcgaa
    29281 aattttcggc agcatcgccg gggcaataca cataaaacca agcggttttc cggcctgatg
    29341 catcgcttgt gccagcgcct ttaattcacg gtcaacggtg cattcgctac caagagtggc
    29401 aaaattgctt aaattcttcg ccgcgccaaa cccccccggc acaatcaacg catccagttc
    29461 agcggcatcg gcctgggcca gaggacggat ttcaccgcgc gttattcgtg ccgcttcaat
    29521 cagcacgttg cgcgtttccg tcatcgcttc gccagttaaa tggttgataa catcaacctg
    29581 ctgcttatcc ggtgcaaagc agaccgcctg cgcaccgctg cgtgaaatag ctaacaacgt
    29641 caacaccgct tcatgaattt cagaaccgtc atagacgccg catccgctca gaattacgcc
    29701 aattttcttc attgtgatca tcctttcgca attgactgaa acacattaat ttttttaata
    29761 aaaatggtac gcatcacaca tttaactgat tcatgtaaca aatcatttaa gttttgctat
    29821 cttaactgcg tgcggcctga aaaacagtgc tgtgcccttg taactcatca taataattta
    29881 cggcgcagcc aagatttccc tggtgttggc gcagtattcg cgcaccccgg tctagccggg
    29941 gtcatttttt agtggctttt gccacccacg ctttcagcac ttctacgtcg tgacgccact
    30001 cttctttcat ctcttcaatc cattcaccga cgttatcttc ccaggcagga aggtccggag
    30061 actgaatttg ctgacccagc tgttgcagat ggcgtaaccc cactgacccc gccgcacctt
    30121 taattttatg tccttcctct acaatgcctt ttttatcctg cgccgtcaga ttcgactcca
    30181 gcacgctgac atagcccggc atcatcttct caaacaccgc taacccgtcg gtgatcagct
    30241 tcggtcctac aagttcgaga tactgttcca gcatgggaat atcgagcaat gcttctgatt
    30301 tactgttctc ttctgtcgtc accgtactct cctcatcatc ctgggtatcc cagaatttct
    30361 tgatcatcgc ggttagcgcc ggaaccgaaa gcggcttact cagcacatca tccattccag
    30421 cattgaggta ctcttgtttg tctttcagca cgttagcggt taaggccacc agcggcggta
    30481 aatcctcgcg cggataacgc ttcgtcagtt cacgagagat atccagcccg gtcatatctg
    30541 gcaactgaat atccagcaac accaggtcgt attcgcccgg tttaaacatc tccagcgccg
    30601 ccttgccggt catggcgaca tcaacgctgt tacctaattt ttccagcaca gaacgcgcaa
    30661 caatcacgtt cagttcaatg tcttccacca gcagcacatt cagcgccggt aaaggcatat
    30721 cgtcttcatc aaacgcatca tcgacctctt ctgctaccga cggtgcgtgg atcgtcaacg
    30781 taaaggttga acctttgccc tgttcgctgg taaccgtaat atcgccgccc atatttttcg
    30841 ccagacgacg agaaacggcc agaccaatac cggtgccggt ggcaggttta ccgccatgac
    30901 tgtctttcac ctggtaatac atggcgaaaa ttttatccag ctcatcctgc ggaatgccga
    30961 taccagagtc ttccacttca aaatgcagca tatcgccttc atcgtagcgc acgcgcacgg
    31021 taacctggcc ttgctgggtg aatttgacgg cgttactgat gaggttccac aggatctgcc
    31081 gtaaacgcgt cccgtcggta atgacctgat gcggtaatgg cagcgtcggc tccaggttaa
    31141 agcgcaatcc tttttgttgc gcctgcaatg cggagagatt ttccagatcg gcaaggaagc
    31201 tggtgaaatc aaccggttga ttatcaagct ggaccttgcg ccgttccatc ttatccatgt
    31261 cgataatatc gttaaagata ttccccagcg tgacggccga aacatggatg gtcttgagat
    31321 atttttcctg ctcggcggtg agttcggtat ccagcagaat acggctcagg ccgacgatac
    31381 cgttcagcgg tgtacgcaat tcgtgactga tggtggagat aaacgtcgtt ttgtcgcggc
    31441 tggcccgttc aagcgcatcc tgataccgct tacgctcggt aatgtcgcga ccaaagccca
    31501 tcaaaccgtg acgtttaccc acgcggtcgt agtacggcac tttacggatt tcaaagcagg
    31561 ctttgcgccc gtccgggtaa tccagccact gttcataggt cagtgacaca ttatgacgga
    31621 acactttttc atcggtttca atgacttttg cggcggcttc cggtgagtaa acatcagcag
    31681 gtttcaggtg aaccagttgt ttttcgcttt ttccggtcag cagctccatc gcgcggttac
    31741 agccggaaaa ctctttatct tcgttacgat aaaaaaccag gtcgggtgaa gcatcaagga
    31801 aggaacgtaa gaatgaggat tgctgctcga gctgaatttg tgtctcttcg cgctctttga
    31861 tttcaatttt cagttggccg aaggtttcct gcagttctgc ttccgctttt tcacgaacgg
    31921 cgatttcctg atttagctgg gcaatattat cttttaactg aacgttgagg ctcaaatcgc
    31981 gctcgcgcat ctcctccagt ttttgcacca gccgtgacag acgttgtcgt gactcctcca
    32041 gttgctcgac gaccaccgat agaaagtaga ccgcccacgg cgtaatcagc aaaccaaaga
    32101 agatagaacg aataacatca atgctttcga cctgaccatg cagcaccatg gttaccgcca
    32161 tttgtaccac aatggcaaga acgacgaggg ccagcgccag caacattgag aagcgcacca
    32221 gacctaactt catcatcagg tcaacataat actgcgccag cagacgaatt tgcttcatta
    32281 gggaattcct tcacgacaac ctgccccaat aatacccaat tctgacagtt acgttataaa
    32341 ttgtgtgaga aatgcggaat tcccctcaac gacctactcc gttggaggta tccacggacg
    32401 ttccagcgcc gatccctgta cgccatgttc attcagatag cgatccagct cgaccattcc
    32461 cgtccatcga ttttcgcacc acagaggagc aagcagcgtc gggcgacggg cgctggcgga
    32521 aatgcggtgg tagatcactt ccggtggcgt atggcgaatc atttctccgg cagtgagcgt
    32581 gtaatcctcc agttcaatac cgttcaaacg ccccgcttcc caggctttcg ccataatgct
    32641 gcctttcaca atatgcagcg gatgcagctt tatgccgtcc acgccggttt caaccactcg
    32701 ctccagcgtt tgcaaacatt cagcctgccc ttcgccaggc aggccgacaa ttaagtggga
    32761 acacaccttc agcccgcgct gacgtgccag ctgggttgta cgctgataac aggcaaaatc
    32821 atggccgcgg ttgatgcgat gcagtgtttt gtcgtgggcg gtttgtagcc ccagctccag
    32881 ccacacttcg taaccctggt ccttatattc gcaaagcaga tccagcaccg catccggcac
    32941 gcagtccggg cgggtaccaa cacacaaacc gacaatattg gcctggctca ccgcctgctg
    33001 atacatagaa cgcagcacct gaacttccgc aaaggtgctg gtatacgcct gaaagtaggc
    33061 cagatagcgt ttagcgcggt taactaaatt cgcctggtgc gccagttgct cggcaatgga
    33121 acgatgctgc tgcgcttcat cggcaaacga ggcaacatta cagaatgtgc agccaccacg
    33181 cccgatggta ccgtcacggt tagggcagct aaaaccgccg tgcagcgtca gcttatgcac
    33241 cttttgccca taacgacggg tgagatcacc accaaacata ttgactaatt tctgtaactg
    33301 cataatctga tagaccgcgc cttgaaaaga ggccaaagcc tgccattttt agctcaattc
    33361 ggcgatgacc tggatcaatc gtcccggcct gcttttatca actgcataat caatcaaaat
    33421 taccgaaatt tcatgcataa tcacataaat cacttttgct tatcttgtgt cagatttttt
    33481 tatctcctta tggattttag gcaaaaacag tagcatgaaa cgtcattacc aattaaggca
    33541 gtataaaatg ctggttttct cgtcagttca aggcaggata agggttaaca cgcctttatg
    33601 acagtcagga attgactgtt tctctaacga cttccctttt agccttaaag ataaaatcta
    33661 ttttaatttc agtcatttaa taaagaattt tgcgctaaag cacatttctg taccaataag
    33721 cttgccattt gacctgtatc agctttcccg ataagttgga aatccgctgg aagctttctg
    33781 gatgagcagc ctgctcatca tatttatgca gtaattgaga tcccctcttc accgtattaa
    33841 ccgatgcgaa aaggacaaca agggggcgaa tgcgaggcgc gcgtatgaca cgcaaacccc
    33901 gtcgccacgc tctttctgtg cccgtgcgca gcggttcgga agtggggttc ccgcagagcc
    33961 tgggggaggt tcacgatatg ttgtacgata aatcccttga gagggataac tgtggtttcg
    34021 gcctgatcgc ccacatagaa ggcgaaccta gccacaaggt agtgcgtact gcaatacacg
    34081 cactggcccg catgcagcac cgtggcgcga ttctcgccga tggtaaaacc ggcgacggtt
    34141 gcggcttgct gttacaaaaa ccggatcgct tttttcgcat cgttgcgcag gagcgcggct
    34201 ggcgtttagc aaaaaactac gccgtcggga tgctattcct gaataaagat cctgaactcg
    34261 ccgctgccgc acgccgcatc gttgaagaag aactgcaacg cgaaaccttg tcgattgtgg
    34321 gctggcgtga tgtccccact aacgaaggcg tgctgggtga aatcgccctc tcctctctgc
    34381 cacgcattga gcaaattttt gtgaacgccc cggcaggctg gcgtccgcgc gatatggagc
    34441 gccgtctgtt tatcgcccgc cgccgcattg aaaagcgtct cgaagccgac aaagacttct
    34501 acgtctgtag cctgtcgaat ctggtgaaca tctataaagg tctgtgtatg ccggcggatc
    34561 tgccgcgctt ttatctggat cttgcggacc tgcgtctgga atcggccatc tgcctgttcc
    34621 accagcgctt ctccactaac accgtaccgc gctggccgct ggcgcagccg ttccgctacc
    34681 tggcgcataa cggtgaaatc aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta
    34741 cctataaatt ccagacgccg cttatccctg atctgcacga cgccgcaccg ttcgtcaacg
    34801 aaaccggctc tgactccagt tcgatggata acatgctgga actgctgctg gcaggcggga
    34861 tggatatcat ccgcgccatg cgtctattag taccacccgc ctggcagaac aacccggata
    34921 tggacccgga actgcgtgcc ttctttgact ttaactccat gcatatggag ccgtgggatg
    34981 gcccggcggg catcgtgatg tccgatggtc gcttcgctgc ctgtaacctc gaccgtaacg
    35041 gtctgcgtcc ggcacgctac gtcattacca aagacaagct catcacctgc gcctctgaag
    35101 tcggtatctg ggattatcag cctgacgaag tggtcgaaaa aggccgcgtc gggccaggcg
    35161 aactgatggt gatcgacacc cgcagtgggc gtattctgca ctcggcagaa accgatgacg
    35221 atctgaaaag ccgccatcca tataaagagt ggatggagaa aaacgtccgc cgactggtac
    35281 cgtttgaaga tctgcccgat gaagaagtgg gtagccgcga actggacgac gacacgcttg
    35341 ccagctacca gaaacagttt aactacagcg cggaagagct ggactccgta attcgcgtac
    35401 tgggcgaaaa cggtcaggaa gcggtcggtt cgatgggcga tgatacccca ttcgccgtgc
    35461 tctccagtca gccgcgcatt atttacgact acttccgcca gcagtttgcc caggtgacta
    35521 acccgccaat cgacccgctg cgtgaagcgc atgttatgtc gctcgccacc agtatcggtc
    35581 gtgaaatgaa cgtcttttgc gaagcagagg gccaggcgca ccgtttaagc tttaaatcgc
    35641 cgattctgct ctactccgat ttcaaacagc tcacgacgat gaaagaggag cactaccgcg
    35701 cagatacgct ggatatcacc tttgacgtca ctaaaaccac gctcgaagcg acagtcaaag
    35761 agctgtgcga caaagccgaa aaaatggtac gtagcggcac cgtgctgctg gtgctctccg
    35821 accggaatat cgctaaagat cgcctgccgg ttccagcccc gatggcggtt ggcgcgatcc
    35881 agacccgtct ggtcgatcaa agcctgcgtt gcgatgccaa catcatcgtc gaaaccgcca
    35941 gcgcccgcga tccgcaccac ttcgccgtgt tgctgggctt cggcgcgacg gctatttatc
    36001 cataccttgc ctatgaaacg ctgggccgcc tggtagacac ccatgcgatt gccaaagatt
    36061 atcgtaccgt gatgctcaac taccgtaacg gcatcaacaa aggcttgtac aaaatcatgt
    36121 ccaaaatggg catctccacc atcgcctctt accgctgctc gaaactgttt gaagcggtcg
    36181 gtctgcacga tgatgtagtg ggcctgtgct tccagggggc ggtcagccgc attggtgggg
    36241 cgagctttga agacttccag caggatctgc tgaacctgtc gaaacgtgcc tggctggcgc
    36301 gtaagcccat cagccagggc ggcctgctga aatacgtcca cggcggcgaa taccacgctt
    36361 acaacccgga cgtggtgcgc acgctgcaac aggcggtaca aagaggcgag tacagcgact
    36421 atcaggaata cgcgaagctg gttaatgagc gtccggcaac cacgctgcgc gatctgctgg
    36481 caattacgcc gggtgaaaac gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt
    36541 ttaaacgctt tgataccgcc gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg
    36601 cgctggcgga agcgatgaat agcatcggcg gtaactcgaa ctccggtgaa ggcggcgaag
    36661 acccggcgcg ctatggcact aacaaagtgt cgcgcatcaa gcaggtggct tctggtcgct
    36721 tcggcgttac tccggcgtat ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg
    36781 gcgcgaagcc gggcgaaggc ggtcagttgc cgggcgacaa agtcactcct tacatcgcca
    36841 aactgcgcta ttcggtgccc ggagtgacgc tgatctcccc gccgccgcac cacgatatct
    36901 actctatcga ggacttagcg cagctcattt tcgacctcaa gcaggttaac ccgaaagcga
    36961 tgatctccgt gaagctggtt tccgaaccgg gcgtaggcac catcgcgact ggcgtggcaa
    37021 aagcttatgc cgatttaatc accatcgcag gctatgacgg tggcaccggc gcaagcccgc
    37081 tgtcgtcagt gaaatacgca ggttgcccgt gggagctggg gcttgttgaa acccagcagg
    37141 cgctggttgc caatggtctg cgtcataaga tccgtttgca ggtcgatggc ggcctgaaaa
    37201 ccggtgttga tatcatcaaa gcggcgattc tcggcgcaga aagcttcggc ttcggcactg
    37261 gcccgatggt ggcgctcggc tgtaaatatc tacgtatttg ccatctgaac aactgcgcaa
    37321 cgggtgtagc aactcaggat gacaaactgc gtaagaacca ctatcacggc ctgccgttca
    37381 aggttaccaa ttactttgaa tttattgccc gtgaaacccg cgagctgatg gcacagctgg
    37441 gcgtaacgcg tctggtggat ctgattggtc gcaccgacct gctgaaagaa ctggacggtt
    37501 tcaccgccaa acagcaaaaa ctggcgctgt cgaagctgct ggagaccgcc gaaccgcatc
    37561 caggtaaggc actctactgc accgaaaaca acccaccgtt tgataacggc ctgctgaacg
    37621 cgcagttgct gcaacaggcg aaaccgtttg tcgatgaacg ccagagcaaa accttctggt
    37681 tcgatattcg caacaccgac cgttctgtcg gcgcgtcgct ttcaggctat atcgcccaga
    37741 cgcacggcga tcaggggctg gcagccgatc ctatcaaagc gtacttcaac ggcaccgcag
    37801 gccagagttt cggtgtgtgg aacgcgggcg gcgtggaact gtacctgacc ggggatgcca
    37861 acgactatgt cggtaaaggc atggcgggcg gcttaatcgc cattcgtcct ccggttggtt
    37921 ccgccttccg cagccatgaa gcaagtatta tcggcaacac ctgcctgtat ggcgcgaccg
    37981 gtggtcgtct gtatgccgca ggccgcgcgg gtgaacgttt cggcgtgcgg aactccggtg
    38041 ctatcaccgt ggtagaaggc attggcgaca acggctgtga atatatgacg ggcggtattg
    38101 tctgtattct gggtaaaacc ggcgttaact tcggtgcggg catgaccggc ggcttcgctt
    38161 acgttctcga tgaaagcggt gatttccgca aacgcgttaa cccggaactg gtcgaggtct
    38221 taagcgttga cgatctggcg atccatgaag agcatctgcg cggtcttatc accgagcatg
    38281 ttcagcatac tggctctcag cgcggtgaag agattctggc gaactggtca accttcgcca
    38341 ctaaatttgc gctggttaaa ccgaagtcca gtgatgtaaa agcactgctg ggtcatcgta
    38401 gtcgtagcgc agcagagttg cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga
    38461 atgtttatca atttatcgac ctgcagcgcg ttgatccgcc aaagaaaccg ctgaagatcc
    38521 gcaaaattga gtttgttgaa atttacgagc cgttttccga aggccaggcc aaagcgcagg
    38581 ctgaccgctg cctgtcgtgc ggcaacccat actgcgagtg gaaatgtccg gtacacaact
    38641 acatcccgaa ctggctgaag ctcgccaacg aggggcgtat ttttgaagcg gcggaactgt
    38701 cgcaccagac caaccccctg ccggaagttt gcggacgagt ctgcccgcaa gaccgtctgt
    38761 gcgaaggttc ctgcactctg aacgatgagt ttggcgcggt gaccatcggc aacattgagc
    38821 gttatatcaa tgataaagcg ttcgaaatgg gctggcgtcc ggatatgtcc ggcgtgaagc
    38881 aaaccggtaa aaaagtggcg attatcggcg caggcccggc aggtctggcg tgtgcggatg
    38941 tcctgacgcg taacggcgta aaagccgttg tcttcgaccg tcatccagaa attggcgggc
    39001 tgctgacctt cggtattccg gccttcaagc tggaaaaaga ggtaatgacg cgtcgccgtg
    39061 aaatcttcac cggcatgggt attgaattca aactcaatac cgaagtgggc cgcgacgtgc
    39121 agctggacga tctgctgagt gattacgatg ccgtcttcct tggtgtcggg acttatcagt
    39181 caatgcgcgg cgggctggaa aacgaagacg ccgatggcgt gtacgcagcg ctgccgttcc
    39241 ttatcgccaa caccaaacag ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca
    39301 gcatggaagg caaacgcgtg gtggtccttg gcggtggcga cactgcgatg gactgcgtgc
    39361 gtacgtccgt gcgccaggga gcgaagcacg ttacctgtgc ctatcgtcgt gatgaagaga
    39421 acatgccggg ttcccgccgc gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat
    39481 tcaacgtcca gccactgggg attgaagtga acggtaacgg taaagtcagc ggcgtaaaaa
    39541 tggtacgtac cgaaatgggc gaaccggatg ccaaaggccg tcgccgcgcg gagatcgttg
    39601 caggttccga acatatcgtt ccggcagatg cagtgatcat ggcgtttggt ttccgtccac
    39661 acaacatgga atggctggca aaacacagcg tcgagctgga ttcacaaggc cgcatcatcg
    39721 ccccggaagg cagcgacaac gccttccaga ccagcaaccc gaaaatcttt gctggcggcg
    39781 atatcgtccg tggttccgat ctggtggtaa ccgctattgc cgaaggtcgt aaggcggcag
    39841 acggtattat gaactggctg gaagtttaag ctctgtccca gcgccgcctc ctcagaaaag
    39901 agtcggcgct gaaattctcc tttctttctg gaaagcgcct cgcataatct gaagaaaacc
    39961 aatgtaatac gctcatctgg attgttaccg gtagatgccc ggcgtatagc tgattccatt
    40021 attcaacgca gcgccatata aagggacaga taatggaatc gctctcagag agaacctcaa
    40081 caggctacca gcaaatccac gacggtatta ttcatctggt cgatagcgcc cggacggaaa
    40141 cggtacgtag cgttaacgcg ctgatgaccg cgacatactg ggaaattggc cgacgaattg
    40201 tcgaatttga acaaggtggc gaggccaggg ctgcgtatgg tgcgcagcta atcaagcgac
    40261 tatcaaagga tttaagtcta aggtataagc gtgggttctc tgcaaaaaac ttacgccaaa
    40321 tgaggctttt ttacctcttt tttcaacatg ttgaaattcg ccagacagtg tctggcgaat
    40381 taacacctgc cctggtccac ctacgtccat ttactttccg ttaaaaacgc tgacgcccgc
    40441 agcttttatg aaaaagagac gctccgctgt ggctggtctg ttcgccagct agaacggcaa
    40501 attgcgaccc aattttatga gcgaacacta ctgtcacatg acaaatcagc catgctgcaa
    40561 caacacgctc ctaccgagac gcatattctt ccgcaacagg cgatacgcga tccctttgtg
    40621 ctcgaatttc tggaattgaa agatgaatat tcagaatctg atttcgagga ggcgctgatc
    40681 aaccacctga tggatttcat gctggaactt ggggatgatt ttgcctttgt tggtcggcag
    40741 cgaaggttac gcattgatga caactggttt cgtgtcgatc tgctgttttt ccaccgccgt
    40801 ttacgctgcc tactaatcgt cgatctaaaa gtgggcaaat tcagctatag cgatgccgga
    40861 cagatgaata tgtatctcaa ctacgccaaa gagcactgga cgctaccgga tgaaaatccg
    40921 cccatcggtc tggttctctg tgcagagaaa ggagccggag aagcacatta tgctctggca
    40981 ggtttgccta acaccgttct ggcaagcgaa tataagatgc aactacctga tgagaaacga
    41041 ctcgcagatg aactcgttcg aacacaggcg gtgctagagg aaggctatag acgccgttaa
    41101 tttcagcggt ttcgaattct gcagacagtg tctgcagaat ctttaaaacg aactaagttt
    41161 taagccatta aatcagcctt aaccttcacc acaacctttt taatctctcc aggctcgccg
    41221 acaacgcatc ctggtttatg cggttcacct ggcataaaca cggcgaacat tcccggtttt
    41281 aagatgatgg cttgctcgtt atcaatggtg ctgcaaagct gataatcatc ctcatggtgg
    41341 aactcttcac actggcgcgc agtgcctgcc atgccaaaca gaatccgttc ctcaccgttt
    41401 aataacagtt ggatatcaat gtattgctcg tgcaattccg cttttttctc gacgggcgat
    41461 tgagtgttaa acgtcatgac attcataaag atattgtcgc cctgtaattc gtaacgaccc
    41521 ggcgcttttt cttgcggtct ggcagctaat gccagcgtta acgcgtcctg taacgcagga
    41581 tgtaacccag cagacggtaa tgactgtact tcacccatca tcataatttt tctccctggg
    41641 ccaacagcgc agccccaagt aaacctgcat cgtggcggta gtgcgccgcc agtaaatcaa
    41701 catgaaatgt cgctggctcc tgcgccagat acgtttccac cagcgccaga tacccttctg
    41761 ccagcccaac gctgccaccg accaccacgc actggcaatc agttgtggct ttaatatcag
    41821 cgatcagcct tgcaagcgta cgtgcggagc ggtgaatcag ctgctgcgcc tgctcgtcgc
    41881 cctgcccggc gcgtgtgaaa atagttctcg catccgcgcc cgccagttca ccctgcgctg
    41941 ccgccgcaat gccgcgacca gaagcaatcg cctccacgca acctgtgcgt ccacagccac
    42001 agaccgggcc gtgtggatcg gcaagcgtat gcccgatgtg ccccgccaga ccgccagggc
    42061 cggtaagcag tttgccgccg ctcactacac cgccgccgac gccagtggaa acggtgataa
    42121 agaccatatc ggttatatcg ccttccagcg cctgatactc tgcccatgct gcggcctgcg
    42181 cgtcgttaat ggcaatggtc ggcaaattgg taagttgttc cagcgttttg actaacggaa
    42241 agtgtagcaa tccaccgaga ttatgcggat tcagcgccag caaactgccg tcgcgaataa
    42301 ttccggttga agcgatggca acccgctgcg catgagcttg caacggagag actaatgcgg
    42361 ataaggcatc acgcaaggct tctggtgtct ggctggctgg cgtaggaagt tcacgacgat
    42421 cgcggatctg cccgtcagcg ccaatcagcg cggcggcaag tttagtaccg ccgatatcaa
    42481 tcgccagcgt ggtcatagca ccgccttttt catcgctgtg ttgtaccact gacaaatgtg
    42541 ttcaagacgc gtaatggcag aaccgaccgt caccgcccac gcgccgtggc gcatcgcatc
    42601 tgccgcctga gcaggcgtgt tgtaacgccc ttcggcaatc accctacatc cggcgtcgct
    42661 caacgttttc accagcgcca gatccggctc ttctggcgtt tcaggcgtgg tatagccaga
    42721 aagcgtagtg ccaataattt cggctcccag cttttggcat gccaggccgt cttccggcgt
    42781 tgagcagtcg gtcatcgcca gtaaaccgtg atggtgaata cgtgccagca gcgtttcaac
    42841 aggcaccgga cgcgggcggt cggtgccgtc aatggcgata atgtccgcgc ccgcctgcgc
    42901 cagcgcatca acatcttcaa tataggccgt gatgcgtacc ggagaatcct ccagatcgcg
    42961 tttcacaatt ccaataatcg gcacgctcac caccgcacgc gtggcttgca gatttgccac
    43021 accttcaatg cgaatggcaa ccgcgcccgc ctgttctgcc gctaatgcca tggcggcgac
    43081 gatttcgggt ttatcgagcg ggctgtccgg aaccggctgg caggagacaa tcaggccacc
    43141 gttagcagcg attttttgat ccagttgtgc aagtaacgac atacatcttc ccttagcgaa
    43201 aggcccggta catagaccgg gcaacaggat taacttttgg ttttgactaa atcgtttttg
    43261 gcgctgccaa atggcacggc accgctaaat ggtttgccgt cgatagcgtc atgagtacgc
    43321 aatgcttccg ggcgcagcca acgctgaacg cgggaaggca tatccagacc aatcagcagg
    43381 atcaccacaa atgtcagact gaacgatagc gatgccagcg cagtacccag atccagacgt
    43441 tgagcgatca acgcgccgat gattggggcc agtgcgccgc ccaatgcacc aacgttgtag
    43501 gtaaagccca ggcccgctgc acgctggtcg gtatcgaaat aaccgccaat cagtttcggt
    43561 aagatcccgg cgatcccttg tccaagcatt tgctggaaga acagtaacag accgagcacc
    43621 cagacgtttg cgccgccaat cgcaaatacc ggaataatca acagctgcga ggccaacagg
    43681 ctacaaacgt acgctttgcg ggttcccagc cagtcaccga ggaagccgcc gacacagcac
    43741 cccaccgcag ccccaaagcc gctaaagaac agcacattgg cgacagtatg cgggttataa
    43801 gccagatcgg ttttcagata cgttggcagc agcgcctgaa tcggccatga gtagaggaaa
    43861 gcaaacaaca cgaccaccat cagcattacg cccgttggcc agcgtttacc cgtactttgc
    43921 accataaagc tgataaagat tgcggcgcat aacagcccaa gaacagcgac gatcgcggca
    43981 ttttgcaggt taccggcgaa gcaaaaccac agcgcagtag ccgccgccag tgtcattacg
    44041 atattggcaa tgcgatgttc accacggtag agaatatcta ccatcgtgcg tactggtgct
    44101 ttacctgcat gtttctcttt ccagtcttcc gcttccggga tgtttttacg cagccagaga
    44161 gcaaagatga taggcaaaat gccgataaag aacagcgcac gccagcccca gaccggaacc
    44221 accaggctat agacctgagc ggcaacgacg gcccccacag agaagcctga aatcagaaaa
    44281 ccactggctt tgttacgcag atgttttggc cagctttcaa tgacataggt ggcgctggaa
    44341 ccgtattcac ccgccatccc catgccgatg accagacgcg cgataaacat ggtgatgtag
    44401 cctggcgcaa agccgcaggc cagcgtcccg gccgagaaga gaacgatgct ggtgaccatt
    44461 gccagacgac gcccgtagcg gtcacccata gcgccgagca tcaggccgcc gaaccagcga
    44521 gagataaagg ctgcggagat cagacttgcc gcctgcaccg tcgtcagccc gaattcacct
    44581 tgtacttcgg tgagtaccag ggcgattaaa acgaaatcaa aaccgtcaag cagatatccc
    44641 aaccaggcag cggaaaatgc ccgccattgt gcacgattga gatggcgata ccacgggata
    44701 ttctgggttg tagtactcat tgtgagtctc ccgcggtggg cgatgcccac acgctttggt
    44761 atgaaaattg tagggtacag atgcgtttat ttcccctcac ccggtagggg cgagcgaggg
    44821 gaaaacaact cacccgcgct cttgcatcaa ctgctgggcc agcgccttca gttctggcag
    44881 atatttttca tctaccggtc caaacggttt gcggcacagc ggcacagaaa cgacatccat
    44941 ataatggagg acagttttca ggccgcggaa tacgcccgtt ttgatcagta aatcaatgac
    45001 tttattgcat tcagtttgca gtttctgcgc ggtctggata tcgccttctt tcagcgcctt
    45061 aacgatcccc tgatagcgcc agcccatgat gttgtaggta ctaccgatac caccatcagc
    45121 gcccgtcagc agaccagagg cgaagatttc gtcgtaaccg ttatagagca caagatcagg
    45181 atgttcacga cggatctgct ccatctgata gagatcgcca gaagtctgtt tcagcgcacc
    45241 gacgccaggc aacgtaacca gggtgttgat ctgatccagg gtcagtttta ccccactcag
    45301 ggctggaatg ttgtacacca ccatcggcaa accatccgct gaatcaataa ttgcccgata
    45361 gtgatcgcag tgttcttcaa agctgaaagg atagtagaac ggcgtgacgg cggagacggc
    45421 atcgaagcca taacgtttag ccgatgccgc aagttgttgg ctttcggcgg tgctgacgca
    45481 accgacgtgg gcgatgagtt taatcttacc tttcgcctct tcggcgacga tttccagtac
    45541 ctgttcacgc tcggaaaggc tttgtacaaa ggcctcgccg gtcgaaccac ccacgtataa
    45601 accgtcgatg ccctgctgaa tattgaactg aaccaggcga cgcagactcg ctttatccag
    45661 tgcttgttgt tgatcaaaag gagtcaggag tgcagccatt acgccacgta aattcgttgc
    45721 cataaatacc tctgaagtga tgcttgtctg ataaacgata tacctttata cctgttatac
    45781 cagatcaatt aagcaacacc ccatacagaa agcttataat gcgatctgct tcactaaagt
    45841 ggcattattt ctttttgttg gtggtctgac cgaaagcgtg ccaggtagca gagacgctgt
    45901 tgagatgcga ttgcaacgca cgatcggctt cgtcaggatc atgacggcgg atcgcatcaa
    45961 cgatcgcaat atgctgttga taactaacgt tgttatgttc gtgcagtgcc tgatcggtaa
    46021 ccgttgggcg tgcggcaata agccagtcga gcagggcaac gtggatcgcc atgaagattg
    46081 ggttaccggg gatctccgcc agcacgcggt ggaaatcaac gtctgaacga atgaatgccg
    46141 cgttgttatc cagcgactga ctgttaattt ccagtgcttt tgccagcaaa tcgatttgct
    46201 catcggtggc atgttcagcc gcatagcgca ccaggctgga ttcaaagaac agacgtaatt
    46261 gttcgaaatg ggcaatccca ccaggatgag aaaggaaatc tttcgccatg ccggaaagct
    46321 cgccgatgat ggtgtccgca gaaggacgtg agacgcgggc acgttcgccg ttgttgattt
    46381 gcaccagacc tttgcgtttt aacgctgcca gcgcttcgcg cacagaagga cgcccgacgt
    46441 taaagaacgc catcagttcg cgttcagacg gtaattgttc accttcgcca aattcacgac
    46501 ggcggatcat ctgttccagc tcttcttcca ccatttcgga gagtttttta cgcgccagcg
    46561 ggcggctacg caagttgcga ccaattgcag gtgaagaatc ttcggtttgc gaatcaaatg
    46621 cgttcataag gcccattctg taaggtcagt gtgattaaca tcatcagtga catcctatca
    46681 caggattgaa agtaggggaa aatggcaggt ttttctcttt gtgcctcatc attaccataa
    46741 ttaacggaat aattaactat tgcgaaaaat taatgtaacg cagataaaaa catcccgttt
    46801 gaattattta taagactatt cacgagcatt atgaatatta tgaatgtgtt cttacaaaat
    46861 aatcataagc gcatattttt taatgaaaaa tcacctcacc tacaattaaa aacacgacat
    46921 ccgcaccata aatagacttg caaaaaatat aacatcattg ttttcaatct gccgtttatg
    46981 ggattgaccg ttttcttttg acacggagtt caacaatgtt cggcataatt atatctgtca
    47041 tcgtattaat tacgatgggc tatttgatcc tgaaaaacta caaacctcag gtggtgctgg
    47101 ctgccgcagg tatcttcctg atgatgtgcg gtgtctggtt agggttcggt ggtgtactcg
    47161 atcccgccaa aagcagcggc tacttgatcg tcgatattta taatgaaatc ctgcgcatgc
    47221 tgtccaaccg cattgccgga ttggggctgt cgattatggc ggtgggcggt tatgcccgct
    47281 acatggagcg cacaggagcc agtcgcgcga tggtgagctt gctaagccgc ccgttaaaac
    47341 tcattcgctc gccgtatatt attctgtcgg caacttacgt catcggccaa atcatggcgc
    47401 agtttattac cagcgcctcc ggcctgggta tgttgctgat ggtcacctta tttccgacgc
    47461 tggtgagtct gggagtaagt cgcctctctg cggtggcggt tatcgcaacc acgatgtcca
    47521 ttgagtgggg gattctggaa acgaactcca tttttgcagc ccaggtcgcg ggaatgaaaa
    47581 ttgccactta cttcttccac taccagcttc cggtcgcctc ttgcgtcatt atctcggtgg
    47641 cgatctccca ctttttcgtg caacgcgctt ttgacaaaaa agataaaaat atcaatcacg
    47701 aacaggcaga gctaaaagct ctcgataatg tcccgccgct ctattacgcc attttacctg
    47761 tgatgccgtt aatcttgatg ctcggctcgc tgttcctcgc ccacatcggg ctgatgcagt
    47821 cagaactgca tctggtggtg gtgatgttac tgagtttgac ggtgacgatg tttgttgagt
    47881 tcttccgcaa gcataacttg cgcgaaacaa tggacgatgt gcaggcgttt tttgacggca
    47941 tgagtacgca gtttgccaac gtggtaacgc tggtggtcgc gggtgaaata tttgcgaaag
    48001 gcttaacgac gattggcact gttgatgcgg ttatcagggg tgcggagcat tctggtctgg
    48061 gcggtattgg cgtgatgatt attatggcgc tagtcattgc catttgtgcc attgtgatgg
    48121 gctctggcaa tgcgccgttt atgtcatttg ccagtcttat tccgaatatc gcagccggac
    48181 tacatgtacc agcggttgta atgattatgc cgatgcattt tgccacgacg ctagcgcgcg
    48241 cggtttcgcc gattactgcg gtggtggtcg ttacgtcagg aattgcaggc gtttcgcctt
    48301 ttgcggtggt gaagcgaaca gcgatcccca tggcagtcgg tttcgtggtg aatatgattg
    48361 ccacaatcac gctattttat taagccatta aaagacaaaa caggccacca gggcctgttt
    48421 ttgtattact tcacaacgcg taatgccggt cgaccaccgc gtggtggctg cggaggttca
    48481 tcgtcaggat gagtgtcatc atcgtgatct ggcttgtcgc catcaataac cgacataacg
    48541 gtttcgttgt ctgccgatgc ctcttcatca ttcatgatgc tggtatcttc atcgtaggca
    48601 gcttcaggct caaacatcgt gcctgcgcca ttttcacggg cgtagatagc cagcacggca
    48661 gccagcggca cagaaacctg acgcggaatg ccgccaaagc gcgcgttaaa gcgtacctca
    48721 tcattcgcca gttccagatt gccgacagca cgcggcgcaa tgttgagtac gatttgcccg
    48781 tcacgcgcat attccatagg aacctgcacg ccagggagcg tcacatccac caccaagtgc
    48841 ggcgtgagct ggttatccag caaccactca tagaatgcac gcagcagata gggacgacgt
    48901 ggtgttagct gtgacaaatc catacagatt aactccggcc cagacgcatt tcacgttctg
    48961 cttcagttaa agaagcaagg aaagagtcac gctcaaagac gcgggtcata tagcctttca
    49021 gctctttcgc acccgggccg ctgaactcga tgcccagttg cggcagacgc cacagcagcg
    49081 gagcaagata gcaatcgacc aggctgaact catcgctcag gaagtacggc ttctgaccga
    49141 agaccggcgc aatcgccagc agttcttcgc gcagttgctt acgtgcggca tctgcttcag
    49201 aagctgaacc gttgatgatg gtgttcatca gcgtgtacca gtctttttcg atgcgatgca
    49261 tgtacagacg gctttcaccg cgagctaccg ggtaaacagg catcagtggc ggatgcggga
    49321 aacgctcatc cagatattcc ataatgatgc gagattccca cagggtcagc tcacgatcca
    49381 ccagggtcgg aacgctctga ttcgggttga ggtcaatcag atcctgaggc ggattgtcct
    49441 tttccacgtg ttcgatctcg aaacttacac ctttctcagc cagcacaatg cggacctgat
    49501 ggctatagat gtcagtagga ccggaaaaca gcgtcattac cgaacgtttg ttggcagcga
    49561 cagccatgaa aacctccagg tatagtcaga atttttactg ctaccagcca ccaggtggcc
    49621 agtcagaagt tgtgttaccc agtaaggaac gactctcttt gttcgaaaat caaacaaaaa
    49681 atgagcaata cccgacattt gggcagaaaa ttggatgata gtttaccaga ttttgcgacc
    49741 tttgtggtga gtcgatgccg gaaatgggga aaaagagatg cgctttagtc tgaaatagtt
    49801 gacttagtcc cttattggcg atgtggtttt tgttttgcct gtctgtcaga cggtagcaaa
    49861 aagcaacttt ccaattttta cgctgattca gaatttaacc ataaaaaaac ccgccgaagc
    49921 gggttttttc gcaaattgtt ttctgccgga gcagaagcca attaacgttt ggagaactgc
    49981 ggacgacgac gtgctttacg cagaccgact ttcttacgtt caacctgacg agcgtcacga
    50041 gtaacgaagc cagctttacg cagttcagaa cgcagggact cgtcgtattc catcagagcg
    50101 cgggtgatac cgtgacggat cgcaccagcc tgaccagaga taccaccacc tttaacggtg
    50161 atgtacaggt ccagtttctc aaccatgtcg accagttcca gcggctgacg aactaccatg
    50221 cgagcagttt cacgaccgaa gtactgttcc agagaacgtt ggttgattac gattttaccg
    50281 ttgcccggtt tgatgaaaac gcgagctgcg gaacttttgc ggcgaccagt gccgtagtat
    50341 tgattttcag ccattgccta taatcccgat tagatgtcaa gaacttgcgg ttgctgtgcc
    50401 gcgtggttgt gctcgttacc cgcgtaaact ttcagtttac ggaacatagc acgacccagc
    50461 gggccttttg gcaacatgcc tttaaccgcg atttcaatca cacgctcagg acggcgagca
    50521 atcatctctt caaaggtcgc ttgtttgata ccaccgatgt ggccggtgtg gtgatagtac
    50581 actttgtcag tacgcttgtt gccggttaca gcaactttgt cagcgttcag aacgatgatg
    50641 taatcaccgg tatctacgtg cggagtgtat tccgctttgt gcttaccgcg caggcgacga
    50701 gccagttcag tagccagacg gcccagagtt ttaccggtcg cgtcaacaac ataccagtcg
    50761 cgttttacgg tttctggttt agctgtaaaa gttttcatta aaagcttacc caataaatag
    50821 ttacacgttg gtgaacaccc aaacgtcttc aattgttgag gttcacacga caaagtccgg
    50881 caaacctacc ccttcgaata gcctatgcca gcacacaaaa agttttggga aaaaaacttt
    50941 cttgtaacgt ggggtcgcag gattatagag aagtcggggt caaagatcga cccctttttg
    51001 tgatttgtga caggttttaa cccgccaaat gctcgcgctt cagatactct tcgctttgca
    51061 tctcttgcag acgtgacagg caacgctgga actcaaactt cagccgatcg ccctgataaa
    51121 tttcatacag cggcacttct gcactcacca ctaatttgac atggcgctcg taaaactcat
    51181 ccaccagcgc aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata actggtacat
    51241 caaacaacat gaccgtatga aagagacgtg agagcgcaat atagtcatgc tgactgcggg
    51301 cgtcgacgca cagcgtagta aaagagaccg ccagcgtctg gttctcgacg cccattgtcg
    51361 ccaatggccg atggttgatt tctaacgtcg gtgaattttc tcgtttcgcc cccgccagcg
    51421 ccaaccatag tttatccatc tgcgcccggg tttcatcgtt gagtggcgaa agccacagat
    51481 gcgcctgagt gagtgtacgc agtcgataat caacaccagc gtccacgttc attacatcac
    51541 aatgctgttt aatggcatcg attgcaggca gaaaacgcgc acgttgcagg ccatttcgat
    51601 aaagttcatc cggcggaata tttgacgtcg ctaccagggt aatgccgcgg gcgaacaggg
    51661 ctttcatcag accgccaagt agcatggcgt cggtaatatc agaaacaaaa aattcgtcaa
    51721 aacagagcac gtcagtttcg gctttaaagc gatcggcaat aatttccagc ggatcggtct
    51781 gcccctgtaa ggcagttagc tcttcgtgca cccgcagcat aaaacggtga aagtgcaggc
    51841 gctgtttccg ctctcccggc aggctttgat agaaaaggtc catcagccag gttttcccgc
    51901 gtcctacacc gccccacata tataagccac gcactggcgt atgctttgtg tcttcgcgtt
    51961 taccccacag cttaccgacc cgcgccatta gcccactcgt cctgggggct ggtggcctgc
    52021 tattgatgag ttcttgataa ataatttcca ggcggctgac ggcctctttt tgaacgtcgt
    52081 cgggttgatg gctgccttca ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt
    52141 gcatgatctt attgttattc cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa
    52201 aaaggccgtt ctacactacg cgatatgcag tcgggattcc acttctgtgg aattaacggt
    52261 tatagtggca taatcagccg caggcatgga gcctgaagcc aacaccctac ggaaacaaaa
    52321 gacaacggga gatgttcatg acctgggaat atgcgctaat tgggttagtc gtcggcatca
    52381 ttattggtgc tgtggccatg cgttttggta atcgtaaact acgccagcaa caggcgttgc
    52441 agtacgaact ggaaaagaat aaagctgaac tggacgagta tcgcgaagag ctggttagcc
    52501 actttgcccg cagcgcggaa ttactggata ccatggcgca cgactatcgc cagctgtatc
    52561 agcacatggc gaaaagctcc agcagcctgc tgccggaact gtctgctgaa gcaaacccgt
    52621 tccgtaatcg tctggcagag tctgaagcca gcaacgatca ggcaccggtg cagatgcctc
    52681 gcgactattc tgaaggcgca tccggcctgc tgcgtactgg cgcgaagcgc gactaattta
    52741 tttttcgggc gcagccattg cgccctcctc ttctctccct ccccgactat catttaatct
    52801 ggtgtctcat tgttagccgt ctgaaaattc aataacatca aactgttttg aatctctttt
    52861 cttatcattc aggtacgaga gcaggaataa tgaaaaaaca aacccagctg ttgagtgcat
    52921 tagcgttaag tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg tcgattccag
    52981 gccaggttgc cgatcaggcc cctctcccca gtctggctcc aatgctggaa aaagtgcttc
    53041 cggcagtggt gagcgtacgg gtggaaggaa cggccagtca gggacagaaa atcccggaag
    53101 aattcaaaaa gttttttggt gatgatttac cggatcaacc tgcacaaccc ttcgaaggtt
    53161 taggttccgg tgtcatcatc aacgccagta aaggctatgt gctgaccaac aaccatgtga
    53221 ttaatcaggc acagaaaatc agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac
    53281 tgattggtag cgatgaccag agcgatatcg ccctgttaca aattcaaaac ccgagcaaat
    53341 taacgcaaat cgctattgcc gactccgata aattgcgcgt cggtgatttt gccgtagcgg
    53401 tcggtaaccc atttggcctt gggcaaaccg ccacctctgg cattgtttcc gcattaggcc
    53461 gcagcgggtt gaatcttgaa ggtctggaaa actttatcca gacagatgct tccattaacc
    53521 gcggtaactc cggcggtgca ctattaaacc ttaacggtga gttaattggc atcaacactg
    53581 caatccttgc gcctggcggc gggagcgtcg ggattggatt tgccatcccc agtaatatgg
    53641 cgcgaacact ggcgcagcag cttatcgact ttggtgaaat caaacgcggt ttgttaggca
    53701 tcaaaggcac cgagatgagt gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg
    53761 gcgcgtttgt cagcgaagtg ttgccaggtt ctggctcggc aaaagcgggc gtcaaagcgg
    53821 gcgatattat taccagcctc aacggcaaac cgctgaatag ctttgctgag ttgcgctctc
    53881 gtatcgcgac tacagagccg ggcacgaaag tgaagcttgg cctgctgcgt aacggcaaac
    53941 cactggaagt agaagtgacg cttgatacca gcacctcttc gtcggccagc gctgaaatga
    54001 tcacgccagc gctggaaggt gcaacgttga gcgatggtca gctaaaagat ggcggcaaag
    54061 gtattaagat cgatgaagtt gtcaaaggaa gcccagctgc tcaggctggc ttgcaaaaag
    54121 acgatgtgat cattggcgtc aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag
    54181 tgctggcggc aaaaccggcc atcatcgccc tgcaaattgt acgcggtaat gaaagcatct
    54241 atctgctgat gcgttaatgt cgtaaaccgg gcatcaggct tacgtgtgat gtccggttaa
    54301 ctcgtggtat gctgctgccg ttcccttttt taatgacgcc tccatcatgt ttgtgaagct
    54361 cttacgttcc gttgcgattg gattaattgt cggcgctatt ctgctggttg ccatgccttc
    54421 gctgcgcagc cttaacccgc tttccactcc gcaatttgac agtaccgatg agacgcctgc
    54481 cagttataat ctggcggttc gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg
    54541 tttgaacacc aactctcaca accagcttga gatccgcacc ctgggatccg gtgtaatcat
    54601 ggatcaacgc ggttatatca tcaccaataa acacgtcatc aacgacgccg atcagatcat
    54661 cgtcgcctta caggatggac gtgtatttga agcattgctg gtgggatctg actctctaac
    54721 cgatctggcg gtacttaaaa ttaatgccac tggcggttta cctaccattc cgatcaatgc
    54781 acgtcgcgta ccgcacattg gcgacgtggt actggcgatc ggtaacccgt acaacctcgg
    54841 gcagaccatt acccagggga ttattagtgc tacgggtcga atcggtctga acccgaccgg
    54901 gcggcaaaac ttcctgcaaa ccgatgcttc cattaaccac ggtaactctg gcggcgcgct
    54961 ggtgaactcg ctgggagaac tgatgggcat taacacgctg tcgtttgata agagtaacga
    55021 tggcgaaacg ccggaaggta tcggctttgc gattcctttc cagttagcaa ccaaaattat
    55081 ggataagctg atccgcgatg gtcgcgtgat ccgcggctac attggtatcg gcggacgcga
    55141 gatcgcacca ctgcacgcgc agggcggtgg tatagatcaa ctgcaaggga tcgtggttaa
    55201 tgaagtgtca cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg atctgattat
    55261 ttcggtggat aacaaaccgg ccatctctgc tctggagacg atggatcagg tggcagaaat
    55321 tcgccctggt tcggtgatcc cggttgtagt gatgcgtgat gataagcagt taacgctgca
    55381 ggtcaccatt caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg
    55441 agtctgtgct ccggtttttt attattcgct aattaattac ttattaacga actcttcgcc
    55501 cagggcgata tctttcttca gcgtatccag cataccttcc agtgcgctct gttcaaatgc
    55561 gctcagggta ccgatagatt tacgctcttc cacgccgttt ttacccagca gcagcggttg
    55621 agagaagaaa cgggcgtact gaccgtcgcc ttcaacgtag gcacattcga caacgccttg
    55681 ttcgccctgc agtgcacgaa ccagagacag accaaaacgt gcagctgcct ggcccataga
    55741 cagggttgca gacccaccac cggctttcgc ttcaaccact tcagtacctg cgttctggat
    55801 acgtttggtc agatcagcca cttcctgctc ggtaaaacta acgccaggaa cctgtgacag
    55861 cagcggcaga atggtaacac cagagtgacc gccaataacc ggcacttcaa cttcgcctgg
    55921 ctgtttgcct ttcagttccg caacaaaagt gttggaacga atgatatcca gcgtggtaac
    55981 gccgaacagt ttgtttttgt cataaacacc ggcttttttc agcacttcag cagcaatcgc
    56041 aactgtggtg ttaaccgggt tagtgataat accaatgcac gctttcgggc aggttttcgc
    56101 aacttgctgt accaggtttt tcacgatgcc ggcgttaacg ttaaacaggt cggaacgatc
    56161 catacccggt ttacgcgcta cacctgcaga gataagaacg acatctgcgc cttccagcgc
    56221 cggagtcgca tcttcaccag aaaaaccttt gattttcaca gcagtaggga tatggctcag
    56281 atcgacagcc acaccgggag tcactggagc gatatcatac agagagagtt ctgaacctga
    56341 aggcagttgg gtttttaaca gtagtgcaag cgcctggcca ataccgccag cagcgccgag
    56401 gactgcgact ttcatcctaa actccttatt atattgataa actaagatat gttgctccgc
    56461 tgccgcgacc ttaatccaca aaattgccgt ttacaatgac cacatctcaa gaatgtgtag
    56521 tcacgcaagt ttagcgttta tgcatttaat tgccgtaatc aggaacctaa cttacgtaat
    56581 taacagtcgc ttatgcatta gcgcaacatt ccaacaggtg gtgacaatat accctaccgt
    56641 tcagccaaaa caacatcaat ttgataacaa ttaatttact tttaaacaga atttgcatgc
    56701 cgtgacgcag gcatgtttct caataacgaa atttgataaa atcccgctct ttcataacat
    56761 tatttcagcc ttcttcaggg ctgactgttt gcataaaaat tcatctgtat gcacaataat
    56821 gttgtatcaa ccaccatatc gggtgactta tgcgaagctc ggctaagcaa gaagaactag
    56881 ttaaagcatt taaagcatta cttaaagaag agaaatttag ctcccagggc gaaatcgtcg
    56941 ccgcgttgca ggagcaaggc tttgacaata ttaatcagtc taaagtctcg cggatgttga
    57001 ccaagtttgg tgctgtacgt acacgcaatg ccaaaatgga aatggtttac tgcctgccag
    57061 ctgaactggg tgtaccaact acctccagtc cattgaagaa tctggtgctg gatatcgact
    57121 acaacgatgc agttgtcgtg attcatacca gccctggcgc ggcgcagtta attgctcgcc
    57181 tgctggactc actgggcaaa gcagaaggta ttctgggcac catcgctggc gatgacacca
    57241 tctttaccac ccctgctaac ggtttcaccg tcaaagacct gtacgaagca attttagagc
    57301 tgttcgacca ggagctttaa tctctgcccc gtcgcttctg gcgtcgggga aaatgttgct
    57361 tatccctctc attccccttc tttctcctgc gatacattta acgaatagtg cgttttaccg
    57421 cgacacatca ttcacaaaat gaatacatac ggtaaaaaaa gcacattatg caaaattcat
    57481 tatctaattg aaaatactat aattaataat aaataaccgt gttttttata cttttttgtt
    57541 attaaaattc acatttttaa cacttagtat caaccgaaac agttagcgtg gtattaatta
    57601 gttcaataat tagtgtatac ttgattttgt gatatgggtc acaaaacaaa ggcccagcta
    57661 aaagattatg tcgaggtaaa aatcatgaaa atcaaaacca ctgtagctgc attaagcgta
    57721 ctttctgttc tctctttcgg tgcattcgct gccgactcca ttgatgctgc acaagcacaa
    57781 aatcgtgaag caatcgggac cgtatccgta agtggtgtgg cgtcttcgcc aatggatatg
    57841 cgtgaaatgc tgaacaaaaa agcggaagag aaaggcgcaa cggcctacca gattactgaa
    57901 gctcgtagcg gtgacacctg gcacgctacg gctgaactgt acaaataaac cctcatcgtc
    57961 ttgtccgacg atattgcccc cggttcgggg gctttttttt gcgctaatga cgaacattaa
    58021 aacgcaaatg cccttccagc tcttcctctg cctcatcaaa cagcaatatt aacgcgccaa
    58081 aacggcgacg cgttttctct cccagatgaa caaattcaat ctcaagtggt agcggcagga
    58141 catcgttcat taacacatcc catagtgagt cgagatcgcg taccttatct ttagccagac
    58201 caaaggtttg gctaaagtca cgataaaaat cctcctgact ctcaatctca tcaaaatcaa
    58261 aggtataaat attcatctgt tgccaccgtc acgtttcgcg gcgagttctg ccgtgcatcg
    58321 ctaagtatag ccatgaaaaa accgacgctt ttggcgtcgg ttttgactta actatcggtc
    58381 aacgcatgtt gatacttatg gagcatcccc gccagccgat gaaccggttc cgtcacctgc
    58441 ggtggcgctt gccagatgcg tagcttttcc tggtagattt ccagttcttc cagcaactgg
    58501 ccaaagtacc gacgacgttt atcatcgcta cgggcagata tcacatggtc tgctgtgcgg
    58561 cgcatttgtc ggtgaaacgc cgataaatcc tcgttaaccg ggatcggcgc atcacgcagg
    58621 cgctggtgcg cgataatcat cgtcagcgcc aggcgaaatt tcggcaaatc ccctgggaac
    58681 ttattcatca gcaaaaacag ctgctgataa agtgccggga ggtggttctc tttacgacgt
    58741 gccacattgg tagtcatcgc ggaaacagcg gcagaaacaa actgattaag cagtacacgt
    58801 ccggtcctgt cgcgcgattt atcccgcaca agcaaaataa cggtgaacgc gagcacacag
    58861 ccgacgattt gccctaatgc gctgtcgaga aactgactga aatggaaagt catcgggtta
    58921 tccagcacga taatatttat ggtgctggcc agcgccccca tcgagcccag tcgccgtttc
    58981 tgtacttcta taccgaggaa aaatcccagc actgccaggc taatacacag cagcaacatg
    59041 ctctgttggg tattagggat aatcaccaaa aagtagagca gccctaacgg cagcgcggcc
    59101 agcgtcccgt agataaagtc gatcgccacc atgcgtggat tcggcaaacg cattgccagt
    59161 gacgtcacga ccgcaatcat caccattgca ccactgccgg aagtccagcc cgtccacagc
    59221 cagaaaagcg tgcccagaat gcaggaaagt gtggttcgcc agaagttaac catcgcatga
    59281 tgacgttcgg ctgactctac tttgacttcc ggttcgcctt gcaggatctc ttcttcggtg
    59341 gcgttgattt ttgtgttact gataacgccg cgcttgagaa gctgataacg cgttgccgcc
    59401 gcgacccagc tataaatggt gacaggcgtt tcccgttccc cggtccaggc gataactctc
    59461 cgcaggcgtt tgagctgctt gtggacgtcc tgcgcgattt ctaccggcgt gtcaaaaaat
    59521 tcgcggaaag tatcagtgat caattccggg cgcgtattct gaataagata agtttcgcag
    59581 gattgggtaa tcagcgtcag cgatagcgta ttgatcgctt ttaaacgtcg attggcccgc
    59641 gcccagcggg aagattccat attcaggttg ctgcgcatgc cttgtagcgc cgtggtgcgt
    59701 cgcaccaggt cgccccaggc tttatcgaca acttcaccat cgccatgctt gatacagagt
    59761 tgcattaatt gatattgcgc gaccagcaaa ctttccagct ctcgatccac ttcttgtttg
    59821 atcgatcgcg gagaaaagag caaatccgcc ataatcgcac acacaatacc gataacgatc
    59881 tcgctacaac gttcgacggc aaactgcggc gtaagcaatg gttccggctg aatggtgatc
    59941 acaatgatca gcgcggtata accggccagc ccccacgcgt acgagttttc tattcgtacc
    60001 agcgaggata tccaggtaca aaaaccggcc cagatacagc acaccagaat catcaataat
    60061 ggtgcgcgga tcatcgcaat gatgatcacc agtccggcaa tacagccaat aaatgtgccg
    60121 atgatgcgca aaaagccacg atagcgaata gcgccagaat acggttcacc tcccgcagca
    60181 aaggccggac cggcggcaac aatcgccgct gtcagtaccg cccagcgtgg cgtttccagc
    60241 tggaagtgaa agccaacaaa cagcgccagt acaatggcgg tcgccagttt taccgcaaag
    60301 cgaatatgtt ggttagcaat ggagaaaata cccatcgtga ttaaccaaac tcacgcaggc
    60361 gatgggccat tttacggaag aacgaatcct ggctttcgtc gcgatcttgt ttgccagtga
    60421 ccaccactgt agcagtggtg cccgcaggcc agatgttctc ttgctggttg tcgagacgaa
    60481 tacgaaccgg aacacgttgc gccaaacgca cccattcaag gttagagtcg atggtcgcca
    60541 tccctttgtc gtcacgcgtg ctgctggcgt tggtgacccc agcggcaaca ctatcaacag
    60601 tccctttcag cactttgtta ctgccaagcg gcgtgatctc tgcacgatac cccggacgca
    60661 ccccttccag cttagtttct tccatatagg ccagtacata gaaggagttc tgtttcacca
    60721 gcgcaaccgc cgttgatcct cgagtaataa actcaccggt atagacgttg aggttggtca
    60781 cccagccatc tgctggcgcg cggatcaccg tgcgttcaag atccagtttt gccagatcgc
    60841 gggtcgcctg cgctttcgct aactgatgca gaaccgtttg tagtacgttg ttagcctggt
    60901 cgatctcttc gcgagacatc gcctgcacac cgagacggtt acgacgtccg gcctcctggc
    60961 gtttctcctg tgccagtacc tgataataag caacatcggc ttgcgcttcc tcaagcgcct
    61021 tttgatagcg cggctggtcg atggtgaaca gtatctgtcc ttttttcacc agctggttat
    61081 catgaacatt cacctgggta atgagtccag aaacgtccgg cgcgatcgca acgacgtcag
    61141 cgctaaagcg cgcgtcacgc gtccaggggg attcggtgta atagacccag gcattaaaaa
    61201 ttgcgatgaa ggccagaatg actaatacga ccgtgatggc cgtacgggag aattttctta
    61261 ttagtgtttt cacttcaacc tcaaacgaac agtcgcgata tcaaataaaa caagcagcaa
    61321 tagagcgcgg tgttgaacaa cgccggatgc cagacaaagt cgtagatacc tgttggcaca
    61381 agtacccggc gcaccagcca gaaaatcgcc agtgataaaa gcaattcaaa aaatatcggt
    61441 gggaaggaca gcccaaacac cacgataacg ggaaacagac tcatgttgac cttggttgta
    61501 tagagagagc aggcgttatt attttcagca tctgtcgccg cagagaaggg catggaaagc
    61561 cgggcgagag caacattgct gtagattgat atttaatata ttagcgtaac tgttatgctg
    61621 ttatctatat tatgtgatct aaatcacttt taagtcagag tgaataatgg aacggctaaa
    61681 acgcatgtcg gtgtttgcca aagtagttga atttggctct tttaccgccg ccgccagaca
    61741 gctacagatg agcgtttcgt ccatcagtca gacggtgtca aaactggaag atgagttgca
    61801 ggtaaagctg ttaaaccgta gcacacgcag cattggcctg accgaagccg gtagaattta
    61861 ctaccagggc tgccgtcgta tgcttcatga agtgcaggat gttcatgagc aactgtatgc
    61921 cttcaataac acccccatcg ggacgctacg cattggctgt tcttcaacta tggcacaaaa
    61981 tgttctcgcc gggctgacag ccaaaatgct gaaagaatac ccaggtttga gcgtcaatct
    62041 ggttaccgga attccagccc ccgacctgat tgccgacggt ctggatgtgg tgatccgcgt
    62101 cggcgcgttg caggattcca gcctgttttc ccgccgtctt ggcgcgatgc caatggtggt
    62161 gtgcgccgcg aaaagctatc tcacacaata cggcataccg gaaaaacccg ccgatttaag
    62221 tagccattca tggcttgaat acagcgtgcg gcccgacaat gaatttgaac tgattgcacc
    62281 ggaagggatc tcgactcgcc tgatcccgca agggagattt gtgactaatg atccgatgac
    62341 gctggtgcgc tggctaacgg cgggtgccgg gatcgcctac gtgccgctga tgtgggtgat
    62401 caacgagatc aatcgcggtg agctggagat cctgctgccg cgttatcagt cagatccacg
    62461 cccggtttat gcgttatata ccgaaaaaga taagctgccg ctgaaggtac aggtcgtgat
    62521 caactcgctg acggattatt ttgttgaggt cggtaaattg tttcaggaga tgcacgggcg
    62581 cgggaaagag aagtaattca tattgtactg ttacgttcta caaacctgtg ccaacgggtt
    62641 cccctcaccc taaccccaga ggagcgaggg gaccgttcgt gcacgtagaa agattaatta
    62701 tccttctgaa aataatgaaa ttattacgca gtaccgccaa cagtcaggtt atcaactttc
    62761 agcgttggct ggcccacgcc aaccggcaaa ctttgccctt ctttaccgca gacacccacg
    62821 ccgttatcca gtttcaggtc gttgccaacc atcgaaatct gctgcatggt ttcgataccg
    62881 gaaccaatca acgtcgcgcc tttcaccggc ttcgttactt taccgttttc aatcagatac
    62941 gcttctgaag tggagaaaac gaatttgccg gatgtaatat ccacctgacc gccaccaaag
    63001 ttcggtgcat agataccgta ctcaacggat tcaataattt cctgcggggt cgatttaccc
    63061 ggcagcatat aggtgttggt catacgcggc atgggcagat gggcgtagga ttcacggcga
    63121 ccgttgccag tcggcgtcat ccccatcaaa cgcgcgttga gtttatcctg catgtagcct
    63181 ttcagaatgc cgttctcaat cagcacgttg tactggcctg gcgtaccttc gtcatcaatc
    63241 gccaccgaac cacggcgatc gaccatcgtg ccgtcatcaa ccacggtaca cagttctgaa
    63301 gccaccagct ccccgacctg cccactaaat actgaagtgc cgcggcggtt gaagtcgcct
    63361 tccagaccgt gacctaccgc ttcatgcaac agcacgcccg gccaacctgc gccaagtact
    63421 accggcatgg tgcccgctgg tgcagcaacg gcagaaagat tgaccagcgc catacgcact
    63481 gcttcttttg cccatgcatc cgcacggact tcgccgtcaa gatcggcaag gaagaactca
    63541 taaccaaaac gaccgccgcc gccactggca ccgcgttcgc gtttgccatc ttcttcgacc
    63601 agaacgctca cggaaagacg caccagcgga cgaacatccg ccgccagcgt gccgtcggtg
    63661 gccgcaacca gaattaattc ataaacgccg ctgaggctgg cagtcacttc ttgtacgcgc
    63721 ttgtccgctt cgcgggcaac cttatcgacg cgacgcagga tatccagctt ctcttcacgg
    63781 ctcatgcttt gcagcggatc taccgaggta tacaacgggc tatgctctac cgcgcccagc
    63841 gtctgtactt tgccatcacc actatcacgg acgatggtgc gcgccgcttg cgcactctgt
    63901 tccagcgcca gcaggctgat ttggtcagcg taagcaaatc cggttttttc accactgatt
    63961 gcacgcacac caacgccctg atcgatgttg taagaaccat ctttaataat gcggtcttct
    64021 aaaacccagg attcgtgata gctcgactga aaatagagat cgccataatc aaggcgacgt
    64081 tcggccagtt gaccgaggat cgcgaacaag tcctgatgtt tcaggccgtt cgccgctagc
    64141 aattgttcac ttaccaggtt cagactcatc gtttttgcta ctcgttagtt actgcagtag
    64201 aggatttttt acggctgccg gatgcggcgt gaacgcccta tccggcctac agttatgttc
    64261 cggctcgttg gcctttggca acgattatca tatgagattg gggcaattac gcgccctcgt
    64321 caaatcattg cgctttttct ttacgcggtt ggcgcaacac ttcgttgatt tgcggatcgt
    64381 ccagcggacc cgaaatgtga tagcgcaaaa tggagacttt gctccacagc ggccccagca
    64441 ctttactggc tgcaaatact gccgcgccaa caatggggtt aaccgcaaaa gccgcagcca
    64501 cgcccaccgt cgcagaaatc tctggtgcga caaccgcttc catattcagg tcgcgacgta
    64561 ccagatttac cgaccctttc atggcgatat ccgcctccag gccatccacc agcgtgtcgt
    64621 cggtgtgcat aaccccgtct ttaatccacg cggtgctgcg aatggagtca aaatagaacc
    64681 cttcgccaaa agtgtctctg aaatcaaaac gcagcttacg catcagggca tcaacgctca
    64741 ataagcgcag caattgcccg gcatgtccgg tattgatttc ggtaatttcg cctttaccca
    64801 gttgagtatg aatgatgcca ttcaacgtcg cctcatctgg ttgccagggt gctttgcgcc
    64861 agtgtaaatc gtaatccaca ttaaatgacg actggcgtat gggcgtcgtg acaccaaaaa
    64921 attctgcggc ggcatcaatt ttctgcccgc gcagttttcc tttcagcgag gtacgttcat
    64981 tctccggatt attaacccat tcaccatcgg cagtaagccg cgagaaacca gtatcaatca
    65041 gtccattggt cagcgttaac gtatcgccag aaatggtgat atcactgtca atgcgaccga
    65101 atttttgccc ccagaaccag cactctgtgc atcgtatttg tgcgtccggc cagccacgga
    65161 agttaatgcg ctccgttgtc gggaaaggtg atgacggcgt tgaatcacca cgagttttcg
    65221 ccacgctcgg gttgtaataa agatatttga tattcgccag ccacggcgcg ttattacgca
    65281 tcgctagcgt ggcgttgatt tcacgccctt gcgcctcaac cagggtgcca tttgccgtcg
    65341 gttgcgaaac aatactcagg ttattccatt gctgatttcc cagtgacaac ataggcgtac
    65401 gtaacgttat gtgttgtggg aaactcgctg caccaccgac actctccgca gcgcctttct
    65461 ggaacagggc cagccactcg gcaccattca tcggcggcat attgagttca acgccacttt
    65521 gttccggcaa cggcgggagc gttttactgt ctgccgccca aatagcacga tcgagcgtca
    65581 gcttttgacc aagcaaccag cggctattga aatggttatc cgcaccagcc tgtccggtta
    65641 attcaaagct gttgagattg ccatcaacct taacgtttac cgctagtggt tcacccgcag
    65701 gtttggctaa cggtgaaggt aagtgactac tcacattatt cagatcgccg ttcagctcta
    65761 cgttataggt cgcgccagca tgataaggca gatcaatgcc cactttacca tcccacgcca
    65821 cgctgccact caatgcttcg ttcaccgctt caggcagaac gctggttttc gccggttgcc
    65881 agttaccgtt gagattcacc gcaacctggt aggcttttgc cccttctttg gtggaaaaat
    65941 ccacgttcaa cggctgatta aaccagcttg ctgtcagtgg ttcactttgc agatcgccat
    66001 tgataaagct gaatttaccg ctcaaatttt tcagggtgct gtcgagtggt ttgataaaca
    66061 gactgttatt acgcagcgtc acttcacctt tcgcggttac cagttcgccg ttcagcggga
    66121 tatcaagatg taagcgagca ttcacatcgc cgtcgagctg gagttcttgc agggtcgcac
    66181 ccagagaatc tttcagcggt gtctcatcaa agtaagggcc aacggcttta cccggacctt
    66241 taatgtcagc gtcaatcagc agtttttctt tcgagtagtc agggatcact gcggtgagat
    66301 tactcgcgcg cacgccgccc agattaacgc catcggtttt catccataaa ccgtcgttaa
    66361 taaagtccag ttcaatatca aggttagtta atgcaggcca gtccggctgg aaggcaaact
    66421 tcgcgttgcg cagcggcacc agcacttcaa actgaccttc gttgtgttta taggggaaga
    66481 gttgcggatt gccaccataa accagcgtcg cgttatccgc ttcaccgccc tgaatcgcgc
    66541 cacttaagta atcaaccagg tctttaccca tcaagttttc cgggaaatag cgccaggctt
    66601 gtgaaccatc atcggtactg atgccagcca gaatacccag ccagggttca tcgttagcag
    66661 gttgcaggta acgaaaaccg ccgcgcgcat ggacggcttt ggctttaacg tcaatattac
    66721 gcccatccag ctggaaacct ttgtcattgt tcagccagct tatagttgcc tggccgtcgg
    66781 cgatttccag tggcgcacgg aataccgttt cgtaaggcat ctttgcctgc ttcatcgacg
    66841 cggtaagcaa accattttca acgctgccgg aaagcgtccc ggagaagtgt tccgcacccg
    66901 gtaataattt ccattgcttc caggccagat cgctccacga tgcctgaaaa cgggtcttgt
    66961 ctgccgcctg aagcgggata tccagcgcca gagtgttaat cttgccgctc ggttgtgtgg
    67021 agcgccaaac atcacccagt gcaggtgaaa gtttcgcggc cagcgggcgt atgccctcca
    67081 ggcctgccag ctccagatta ctggcgcgaa tccggagttc gtcactgcgt ttattgtctt
    67141 tgccgccaac gtcctgttcc ggtatccagg ccagcgtcaa tgctccgctc ggccagggtt
    67201 tgccgtccat cgtgatccgt gtatcgggaa tagagaactg ccagcccgga ttttcacgcg
    67261 taatatgcgc ggtcagatta tccaccgaca gcgtatgcgt ttgcttctcg cccaaccagc
    67321 tggcaccgcc ctgtttcagc cagacgtcac cgccggttac atcgcctttg tcgatcgtca
    67381 tccagccttc aagggagaac tgtgccgttt ccagagcaat attgtcctgc atccatttac
    67441 cgagccacgg cttcaggtcg atgtcatccg cctgtagcca gacgcgacca ttgcttaaca
    67501 acccctcatc atcgcgcaaa tccatgcgca cctgcatcac gccgtgctgt ccggtaaggc
    67561 tggagaggct taccaggcct tccgcacggt gtcgacgtgg atcgttcagc caggtgagtt
    67621 gtgggatcgc cagctcggcg cgctgaccgg atggcgtcag aaaactgact tcactgtcgc
    67681 ggagatcgaa atggtcaaat tgacgaagaa acagatcgct gatgtgactg gcttccagac
    67741 tgtcatcgct accaccgctg gtgataggag tgttggtgcg aaagcgcagc tgccagaaag
    67801 tgaggtcgcg aaactgccag cgcatatgta acaggctctg ccagacatcc agcgccagag
    67861 taacgcgttt aaccgaaaat tcgccgccat cttttagttc tgcacggatg tcgtgtgctt
    67921 caagcgtcgg gccaaaattc tgccagctgg ctgagagctg actggcttct accggcatgc
    67981 cagtcgcgga ttctattttg ttgaggattt ccggacgcca ggcgtcaaga tgcggtaaag
    68041 caatacgcag gccgctaacc agcagagcag cgatcacaac gagcgcggct ccagtaagca
    68101 gtaaaatccc cggcaatcgc ctcacccgtc actccttgtc tgctaaaaat gtgactcaaa
    68161 aaccctttgc cggatggcgg cccagcatct gtttacatca ttacgacgtc aaactgctcc
    68221 tggttataga gcggttcaat ttgtacttta acctgtttgc caacgaaaat ttccacttcc
    68281 gccagcgagt gtgactcttc gcctttcaag gcttcagcta ctgccggaga tgcatagacc
    68341 aggaaacggt cggagtcgta agcatggtgg acacgaacaa tctcgcgcat gatttcatag
    68401 cataccgttt ccacggtttt caccgttccg cgaccgtggc aggttgggca ttcgttacac
    68461 agtacgtgct caatgctttc gcgggtgcgt ttacgcgtca tctccaccag ccccagcgcc
    68521 gaaaaaccat taacgctggt tttcacccgg tctttgctca acgcctgctc cagcgagtgc
    68581 agcacgcggc ggcggtgatc ttcattattc atatcgatga aatcaataat gataatccca
    68641 cccagattac gcaaccgtaa ctggcgagcg atagcctgcg tcgcttcaat attggtattg
    68701 aaaatggtgt cgtccagatt gcgatgaccg acaaacgctc cggtattgat gtccacggtg
    68761 gtcatcgctt cggtctggtc gataatcaga taaccaccgg atttcagttc tactttgcgt
    68821 tccagcgctc gctggatttc gttttcgaca tcaaagagat cgaaaatcgg ctggcgtccg
    68881 gtgtaatgct ccagcttgct ggtcatctcg ggaatgtact ccgaggtaaa ctccagcaat
    68941 gcttcgtaag tcaggcgtga gtcaacgcga atgcggtcca gttcggcgtc ggcgaaatca
    69001 cgcagaacac gctgcgccag cgccagttcg ccgtacagct gataacgggt ctgcgggcgt
    69061 tttttacgct ccataacttt ggtccagacg cgtttcagat aagcggcatc ggaggccagt
    69121 tcagcctcgc caaccccttc cgctgcggta cggatgataa acccgccctg ctcgtcgcaa
    69181 tactctgcga ccactttttt caggcgttca cgttctgatt cgctttcaat acgttgggaa
    69241 accccaacgt gagaagcccc tggcataaac accagatagc gagaaggcag cgtgatatcg
    69301 gtggtcaggc gcgcaccttt agtgccaagc ggatctttca ccacctgcac catcagatct
    69361 tgcccctgac gaaccagttc cgagatgtcg cgcaccgtga attgcttttg ttcttcaccc
    69421 gccacacatt cggtgtgcgg catgatatcg gatgcatgaa gaaacgcggc tttatccagc
    69481 ccaatatcta caaaagccgc ctgcataccc ggaagtacac gacttacacg acccttgtag
    69541 atattgccta ctattccgcg tcgcgcctca cgttcaatat gaatttcctg cagaataccg
    69601 ccatcaatat acgccactcg cgtttccgaa ggcgttacgt ttactaacaa ttcagccgtc
    69661 atgtttatcc cttttctcac gcagtgcgtt aaaattactt aataattcat acgtttcaac
    69721 cagcggtaag ccgactacgg cgtgatagct gccatttatc ttcctgacaa aacagccacc
    69781 cagcccctga ataccgtatg cacctgcttt atctaacggt tcaccgctgg cgacatagcc
    69841 cgcgatgtct tcgtctgtta acgttctgaa agtcacatcg gtgaccacca ggcaatcgag
    69901 aatgtgctgg ctgtcggcca acgccactgc cgtcatcacc tgatgggtct gacccgataa
    69961 tttgcgcaac atctgtgccg catgctctgc gtcgcgcggt ttctccagca cttctccatt
    70021 caggataacg atagtatccg cacccagcac cgggagatcc tgcgccgttt gcgcgacacc
    70081 cgcctgtgct ttctcgcgcg ccagacgcac aacatactgc tgcgcgctct cctgcggctg
    70141 acgctgctcc tcaatgcccg taacaatacg ttcaaaggtc acgccaagtt gcgcaagtaa
    70201 ctcctgacga cgcggagaac cggaagctaa atacagagaa gtcatagaaa cctttattgc
    70261 actgcaaact gctgacggac tttacgcatc agcaagaaaa tccacggcca gagcacccca
    70321 ttgactacac tactccagaa cacttccggt ctgaaagaga cgttaatcac taaaaactct
    70381 gcccagaaaa caataatatc caccaccagc gaaagcaaca tgacgaccag cgcctgctgc
    70441 cataatgcga ggttgcggaa aagctggtat ttcagcgcca ccaggtaagc aatgatgctc
    70501 atcgccaata cgcgtacgcc aagcgtcgag ccgctgatca gatccagtat ggcacccatc
    70561 acaaaacctg tgcccacatt tacgcgatga ggcaaggcca ggatccaata caacaagatg
    70621 agtaacaccc agtttggccg gaaaacaatc aggttatccg gccagggcat gatttgcagc
    70681 aacagcgcaa tgaggaaaga gagccagatt acccaacgtc cctggctacg atagctcgcc
    70741 actattgccc tcccggcgca cgcgcaggcg gttgagcacc actttgcggc ggcgtagccc
    70801 tttgtggaga gcgattagca gcaggctgtg tgggcgcagc aggcgcagta gctgcatttc
    70861 ctgtcgtcgg ttgctgcgga gtcggctgag cgatccccgt tgccggttca ggtaactttg
    70921 gccccatcgc gtctggcgaa ggcaatacct gcggcatcat ctgcatcaga cgttcattag
    70981 caacacgatg cacctcttcc ggcgtcatcg ggttagcgcc gttacgatcc gcgccccaca
    71041 gcagcagcag ataacgcaaa cgttgcagtc ctgcagtcgg acgcgcctga atcacagtat
    71101 aagcgcgctg ggtatcgagt tttacggaag agacaaccgc gaccggatag ccttccggga
    71161 aacgaccgcc cagaccggag gtcaccagca catcaccaac acgaatatcc gtattcgctg
    71221 gcagatgctc aagctgcaaa tcatccgtac aaccgttacc ggctgcaatt acgcggatat
    71281 cgttgcgcag cacctggatt ggcagcgcgt gggtcgcatc acaaatcagc agcacgcgac
    71341 tggtcagttt agcgacggcc accacctgac caacaacacc tttgtcgctg atgaccggct
    71401 ggccttcata aacgccatta acgctacctt tatcgataac aacttgatcg ctataaggat
    71461 cgttaaccgt ggagataacc tgagtcacca ttttctgctc atcctgacgc agcggggaac
    71521 ccagcagctc gcgcagacgc gcgttctcct gtttgtattg tccaagcatc agcagttcac
    71581 tgtttttcag caacagttcc tgacgtaacg cccggttttc aagttctagt tggtcacgcg
    71641 aggccagcgt ctgcgatacg ccatccagca attcacgagg agcattggaa acaaagtaga
    71701 aaggactgac ggcggtatcc atataagtac ggatttgact gaacgtcccc aggcggctgt
    71761 cggcaataat aatgccgagc gccaccagca ccgccagaat aaggcgaatc tgtagcgacg
    71821 ggccacggct aaaaattggc ttcataagtt atgcgtattc tcgtatcaga ccaggcaggg
    71881 taaacagaca tttcccctgc ctgcatccga ttactcttcg ctgaacaggt cgccgccgtg
    71941 catgtcgatc atttccagcg ctttgccgcc accgcgcgcc acacaggtca gcgggtcttc
    72001 agcaacaacg actggaatgc cggtttcttc cattaacaaa cggtcaaggt tacgcagcag
    72061 tgcgccacca ccggtgagca ccatgccgcg ctcggagatg tcggaagcca gttccggcgg
    72121 gcactgttcc agtgcaacca ttaccgcgct cacaataccg gtcagcggtt cctgcagtgc
    72181 ttcgaggatt tcgttggagt tcagggtaaa accgcgtgga acaccttctg ccaggttacg
    72241 gccacgaact tcgatttcac ggacttcatc gcccgggtaa gccgaaccga tttcgtgctt
    72301 gatacgttct gcggtggctt caccgatcag agaaccgtaa ttacgacgca catagttgat
    72361 gatagcttcg tcgaaacgat caccaccaat gcgcacggaa gaggagtaaa ccacgccgtt
    72421 caaggagata acagcaactt cagtggtacc accaccgata tcaaccacca tagaaccggt
    72481 cgcttcagaa accggcaggc cagcaccaat tgcggcagcc atcggttctt caatcaggaa
    72541 gacttcacgg gcaccagcgc cctgcgcgga ttcacgaatt gcgcggcgtt caacctgggt
    72601 cgcgccaacc ggtacacaaa ccagaacgcg cgggcttgga cgcataaagc tgttgctgtg
    72661 cacttgtttg atgaagtgct ggagcatttt ttcagtcacg aagaagtcgg cgataacgcc
    72721 gtctttcatt gggcgaatgg cagcaatatt gcccggcgta cggcccagca tctgcttcgc
    72781 gtcatgacct actgcagcta cgcttttcgg tgaaccggca cgatcctgac gaatggccac
    72841 cacggaaggc tcattcaata cgatgccttg tccttttaca taaatgaggg tattcgcagt
    72901 acccaggtca atggacaagt cattggaaaa catgccacga aattttttca acatactaag
    72961 ggataatcct gaaagctggg gcggaaaaga aaatccgctt actttaccaa ccacacgcag
    73021 cagcgacaag gcgcaaaaat catctgctac ggtgaaaatt agtgcagttc gtttcctttg
    73081 ttacaaatct ctgcctgagt ccagaaaggc ttaatgcatc agcagcattc ctcgcctgtt
    73141 tgcaaccgcg taaggtcatt catctgcata tgtgctgcaa caatctggcg agcagacaag
    73201 cacactccca tgagacgcag cgcgcgttat tctacgtgaa aacagattaa acggcaggtt
    73261 aaaccgagta tctttgtgaa tattttttca cgttagtatc aagtggctgt gaggacgcga
    73321 aaaaatcccc ttgcccgcct gtaacaccgc gctgaatcag ggtctgccac tcgcttcgcg
    73381 aacgcacacc ggtggcgtaa acttgggtgc tggtcccgga gcaggcttcc accaggcttt
    73441 gaaccagcag ctggttctcc gttcgcttct caatgtttct gaccagcccc ggatggagct
    73501 tgagtaactc aacattaagt tctttgatcc aactggtgct taccagcgtc aaaccagcct
    73561 ggttgacggc tacccgtacc cctaaagcat tcactaaacg aataacaggt tgtaaacgac
    73621 tgatatgttg acctacatcg gcctctgcaa gttcaataat tatgcgtttt cgttgtgatt
    73681 tttcacattg cattaacgta tcgcgcagcc aacgctgaaa acgcgggcga atcagcgact
    73741 caacggtaac ctgaatcgcc agattttcct ctggccagta acgcaatagt ggaataagac
    73801 ggctgatttg cagacggtca tactcttccg ataagccaaa ctgcaagacc atcggcatat
    73861 actccgccga gctaacctct tcattaccat cgaagatgcg gcacatgagt tcgcgatgat
    73921 gaacctgacc ttcgcgagta accgccggtt tttgataaag gcgcgggccg ccgcgactga
    73981 gcatttgctc gataagcgta cgccagcgaa cattaccgcg tcctttttca ggcaacgagt
    74041 catcgtaaat agcccagcta ttgccgccct gcaatcccgc attacgcgtg gcagactctg
    74101 catgttccat tacctgctcg gtatcctgac cactacgcca ggcgcagata ccaatgtgga
    74161 tcatatcgtc gcgatcgagc attttattgt tcggcaaggt atcgacggct ttgattaact
    74221 gaccggcgat gctctctgcc tcttttaacg tccggtgcgg taacagcgca gcaaaatcac
    74281 tgcggtggta acgcgccagc agtgcgccag ggtagcgcat cataaatgtc gacagcagat
    74341 tcgtcagagt gaagaactgt tcttcaacct ggctgtgccc ccaagtatcg ctcaacatat
    74401 tgaaatccgg cagacgaatc atcatcacga tcccgtgggt acctactttc tcctgatctt
    74461 ccagtaacgt tgctaactga ttatcgaaaa agagtcggtt attgaggccg gttttcacgt
    74521 cctgggcggc ataagagcgg atcagcgtat caagacggct gtgttgttcg cgtgcgttct
    74581 gaatttcacg aagcagcgta tctagcgcac tgctggttct gggcggccat tcatagatgg
    74641 ttcccaacac attagagcca cgctcaccgt ttaagatacg agtagcccgg gtttccagca
    74701 attcttgccc ggcaagttgc cgttgtaacc agcgtaccgc caggaagagc ataacaatga
    74761 taaagccaat cgcccccgtg agcggcgcgg tagtcattaa agaatggaaa tagttgccca
    74821 tcggatcctg ataaaccaga cgcagcgaca tccccggatg ctttatcaac ggaacactca
    74881 gttcgcgaaa cagatcgctg gagccaaccg gacgataact accatttctg gccagggtat
    74941 aaacctgttt atcaccatgg agcaggtcta cacgaacgat atctgccgac atcattaatt
    75001 cggtaatttg tggccttaat gtgctgaagt cattcgacac aaggtgggta tcaatcgccg
    75061 tcgccaccgc ctgaacgcga tgactaaact tatactgaat tgcgttgtag aaacttagcg
    75121 aacagcccag caaagtcaca aaaattgtta acccggtgag cagcgtaaca aaggccgaaa
    75181 atttcgtcgt taatctcatc cttgtgttaa ctccgatagt gaggaagcgg gcatactagc
    75241 aaatcagatt tatctcgcaa tttattgcgc ttcatcggct ttgcttttcc attagcgagt
    75301 atagtcttca gaaattattt tccaatccat catgcacatg aggaccactt atgcaggcgt
    75361 tacttttaga acagcaggac ggcaaaactc tcgcatcggt acagacactt gacgaaagtc
    75421 gcctgccgga gggcgatgtc acggttgatg ttcactggtc gagcctgaac tataaagatg
    75481 cgctggcgat taccggtaag ggaaaaatca tccgtaattt tccgatgatt cctgggatcg
    75541 attttgccgg aactgtacgc accagcgaag atccgcgttt tcatgccggt caggaggtgt
    75601 tactcactgg ctggggcgtt ggtgaaaacc actggggtgg gctggcggag caggcgcgag
    75661 tgaaaggtga ctggctggtt gccatgccgc aagggctgga cgcgcgtaaa gcaatgatta
    75721 tcggtactgc cggttttacc gccatgctgt gtgtgatggc gctggaagat gccggtgttc
    75781 gcccgcagga cggggagatt gtcgtgacgg gtgccagtgg tggcgtcggc agtaccgccg
    75841 tggcgctgct gcataagttg ggttatcagg tcgttgccgt ttccggtcgc gaaagtaccc
    75901 atgaatatct gaaaagttta ggtgccagtc gtattctccc tcgtgatgag tttgccgaat
    75961 cccgtcctct ggaaaaacaa gtctgggctg gggcaattga cactgttggc gacaaagtgc
    76021 tggcaaaagt gctggcgcaa atgaattacg gcggctgcgt ggctgcctgt ggtctggcgg
    76081 gtggttttac tctgccaacc acggtcatgc catttattct gcgtaatgtc cgtttgcaag
    76141 gggtggattc agtaatgacg ccaccagaac gccgcgcaca agcctggcag cgactggtcg
    76201 ccgatttacc ggaatcattc tatacccagg cggcaaaaga gatatctctg tcagaggcac
    76261 cgaactttgc cgaggccatc attaataacc agatccaggg tcgcacgctg gtgaaggtta
    76321 actaaccatt tagcaaggaa taataagaga gggaactcat tttgacatca ttattcaatc
    76381 ggttaacggg aaaagcggtt agccggacag ctttcgtcga acaccttggt caggaagttg
    76441 tacaacatca tccaaactgg aaagtcatga tttcgactga ccacaaattg atgcgcattg
    76501 atacgggtca ccaaacggtg gaaatcaccc ttgatgatgc ctacaaacgt tatcaagccg
    76561 caccagaccc gctgcaagaa gtggctgcag atatgctgaa acagcttgca gaaatgctgc
    76621 cgaccgggcg ctgtattgtt ccggtgatta aagaaaaagc ctggctggat accgttcgcc
    76681 aacagcttag ccagtatgag tctgatccag aggtcctcaa cgcctcgctg gattttgcct
    76741 gggaagactt taacgatgag ttaattatct tgtttgctga agatggtagc gaaaccatgc
    76801 gctatctgat gcgctacgaa gtgaccattg accgcgaaca agccatcagt aatttgaagc
    76861 aaatattgcc ggaagttacg gctgaaatcg ttgatacagg cgcaaacgat ggaaaagttg
    76921 ccgttatctc cgcagggggt acttatgagc ccagtctgat gctcattgac gatctttggg
    76981 tgcaatcacc cgatcctgaa tgcggcgacc tgttagtgac cgttccctcc agaggaaaac
    77041 tgtttttcac ccacaatgat cagccagcaa atctcagtgc attacgacag ctaacaaaac
    77101 aatgcttccg tgaggatgac catcctctga caacacagat attcatccgc cgtaacggtc
    77161 acttttatcc ctgggtagaa accttgcatt aacgcaacct ccgtcatgct tgaaaaagct
    77221 tgtcggagta tgtgatgaaa actgcaaact caccgaagcc gattttgcta ccaaatcggc
    77281 ttttttattg tcaggtggca aattctgaaa gcatcagata gccgcactgc cgcctgacaa
    77341 aacagtttac cttctgcaaa atggggggaa taatctgatt ttgtttgact acaaattaat
    77401 cactcgaacc tatttaatgc tgagcattgt caatcggtta attttgcgtg ctttagcatt
    77461 aacatctatc cagacaatgc agtgaaaatt gggtaatccc cagcaaccgc tgcgtaatgt
    77521 cgtctatctt gtcgcgatcc tggcatccct acattatttg tggtctgtga agattatctc
    77581 attgcagccc ctcatcttcg cagggctggc tttacagctt ttcaccttac gttataagaa
    77641 gttccgtcga tgatggcgct aatttcgtga attgtgcggc ttgttgcaaa ttacacggtg
    77701 ttgaaggtta tttacatgtt agctgttgat tatcttccct gataagacca gtatttagct
    77761 gccaattgct acgaaatcgt tataatgtgc gacctcgtcc tccctgacgc agtttttgcg
    77821 ctgcggaaaa ggtgacattg gcgcaacgaa ggtatatttt gttttttgcc ggaggatagc
    77881 agcagatcgc tgcacaatgt ccgtcaagtc taacattgac actctggggc aaaatagacc
    77941 ggcgtcccgg cctgctggaa tttatcgcta tgcatacagc tgtcggggca tacgctttac
    78001 agacggcggt gaaacgcctg tcacaatcac actaaacaaa gagtacggaa cccactcatg
    78061 gatattcgta agattaaaaa actgatcgag ctggttgaag aatcaggcat ctccgaactg
    78121 gaaatttctg aaggcgaaga gtcagtacgc attagccgtg cagctcctgc cgcaagtttc
    78181 cctgtgatgc aacaagctta cgctgcacca ataatgcagc agccagctca atctaacgca
    78241 gccgctccgg cgaccgttcc ttccatggaa gcgccagcag cagcggaaat cagtggtcac
    78301 atcgtacgtt ccccgatggt tggtactttc taccgcaccc caagcccgga cgcaaaagcg
    78361 ttcatcgaag tgggtcagaa agtcaacgtg ggcgataccc tgtgcatcgt tgaagccatg
    78421 aaaatgatga accagatcga agcggacaaa tccggtaccg tgaaagcaat tctggtcgaa
    78481 agtggacaac cggtagaatt tgacgagccg ctggtcgtca tcgagtaacg aggcgaacat
    78541 gctggataaa attgttattg ccaaccgcgg cgagattgca ttgcgtattc ttcgtgcctg
    78601 taaagaactg ggcatcaaga ctgtcgctgt gcactccagc gcggatcgcg atctaaaaca
    78661 cgtattactg gcagatgaaa cggtctgtat tggccctgct ccgtcagtaa aaagttatct
    78721 gaacatcccg gcaatcatca gcgccgctga aatcaccggc gcagtagcaa tccatccggg
    78781 ttacggcttc ctctccgaga acgccaactt tgccgagcag gttgaacgct ccggctttat
    78841 cttcatcggc ccgaaagcag aaaccattcg cctgatgggc gacaaagtat ccgcaatcgc
    78901 ggcgatgaaa aaagcgggcg tcccttgcgt accgggttct gacggcccgt tgggcgacga
    78961 tatggataaa aaccgtgcca ttgctaaacg cattggttat ccggtgatta tcaaagcctc
    79021 cggcggcggc ggtggtcgcg gtatgcgcgt agtgcgcggc gacgctgaac tggcacaatc
    79081 catctccatg acccgtgcgg aagcgaaagc tgctttcagc aacgatatgg tttacatgga
    79141 gaaatacctg gaaaatcctc gccacgtcga gattcaggta ctggctgacg gtcagggcaa
    79201 cgctatctat ctggcggaac gtgactgctc catgcagcgc cgccaccaga aagtggtcga
    79261 agaagcacca gcaccgggca ttaccccgga actgcgtcgc tacatcggcg aacgttgcgc
    79321 taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact ttcgagttcc tgttcgaaaa
    79381 cggcgagttc tatttcatcg aaatgaacac ccgtattcag gtagaacacc cggttacaga
    79441 aatgatcacc ggcgttgacc tgatcaaaga acagctgcgt atcgctgccg gtcaaccgct
    79501 gtcgatcaag caagaagaag ttcacgttcg cggccatgcg gtggaatgtc gtatcaacgc
    79561 cgaagatccg aacaccttcc tgccaagccc aggcaaaatc acccgtttcc acgcacctgg
    79621 cggttttggc gtacgttggg agtctcatat ctacgcgggc tacaccgtac cgccgtacta
    79681 tgactcaatg atcggtaagc tgatttgcta cggtgaaaac cgtgacgtgg cgattgcccg
    79741 catgaagaat gcgctgcagg agctgatcat cgacggtatc aaaaccaacg ttgatctgca
    79801 gatccgcatc atgaatgacg agaacttcca gcatggtggc actaacatcc actatctgga
    79861 gaaaaaactc ggtcttcagg aaaaataatt tgtgacgcgc ggtgttttca tcgcgcttag
    79921 caaagaacgt aatcaacgcc gggcctgtta acgctgcccg gcgtttttat tgccgttagc
    79981 taacggtagc ctgcaatttc cgctttcaac gcgctcattt cagcatcgcc atcaacaatt
    80041 cccagctcaa agcagtattt ctttgtttcc cctggctcaa taaagggcag acgcccctgc
    80101 gctcgttctt tatcacgacc gtcaggaaaa ctattactga cctccagtcc cataacatac
    80161 tcacccgcgc ctaaattttt ccactggata aagttgggta gctgcatggt gtcgaatttc
    80221 tcgtaaattc cccaccccag ttcggcgttc accagagcaa ctttactcat cccctgactg
    80281 tcgctattca acgtgagata gtaaacctgc tcgtcaaaac cgggctgtgg ggtacaaatc
    80341 tccagatgac tggttaaccc ttccgccgca tgcggagttc gtggcgttac gcttttagcc
    80401 gacagcaaga tttctgcctg tggcgataat aaaggccagc cgtaattaaa gtgataaaga
    80461 atttgcagcg gctggcgggt gaaaccatga ttagtcacct tgtcttcaat gcgcagcaca
    80521 ttttcgccat aacggcagcg aatggtcctt tccagcgtca gattttcgcc gaacagtcgc
    80581 gtttcccgta ctttgccatt gatgacaaat tcaatgccgt cgtcggtccg ttctgtgcga
    80641 taccccacct cttctgctgg agtcgcagcc aaacgcccgt gtaaccccag cgtttctcct
    80701 tcatcctcac aaggcgtgcc aatgtagcta agtccgcagg tagtcagtaa ccccgcgtaa
    80761 aagctacgta aaaatccgtt gccctgagac tcaaaaaaag cgggggcgac aatgccgttg
    80821 ggggtgataa aagaaagcgc cgtgtccttg tagctggccc atgcaatatc cagcgcgcga
    80881 tcttttacca ccgtaaatgc cagtcccgca ccagtacgga catcaaacgc ttctgtaccc
    80941 tttgctctgc cactacgtaa ctcatagtgt ttaattccgg caacctgcga gagatcgcca
    81001 atccacgacc aaatttcgtt tttcattgtg ctgcttcccg aatggacatg tcatggcgct
    81061 ggatgaacgc ttcgatttcc gcaaggcgag acgttccctc cattgggcca cgtctggtta
    81121 ccgccagcgc accgcaggca ttggcatatt gcagcgctcg atgggcgtca aaccctagct
    81181 ggcggcaggc aatccacgcg ccaccaaagc agtctcccgc accggtcggg tcaacttctt
    81241 ccaccggata gctctcaaca tgaaactgct cattcgcgga gtagtaactg gcaccttgat
    81301 tgccgcgctt cacaatcacc tctttaacac cttcttcaag aaaacctgcg attgcacgct
    81361 ccggcgtcga atgtggcgac aagagcaaca cttcgccctc gctgggcata tagatatccg
    81421 ttaactcaag aacaaagtgg agggcgtcac gcatttctgg aatatcgagc atctctttac
    81481 gaatattagg gtcgaaagaa ataacaccac catttgcttt aacgatagtt accgcttttt
    81541 ttactgcgtc taccatatga aacgaaaaca gggaagagcc cataatatga aaatgggtac
    81601 agtccttcag aatattttca tcaacatgct gcgccgataa tttaccgcaa gcggcattct
    81661 taatattaaa aataaaatcg cgatcgccag agttatgata agtaacaaag gcactcccgg
    81721 tagcttccag aggtaagact gaaatccccc tgatatccac accgtcagca gccagacgat
    81781 gtatattaat atcaccaaag ccatcattcc ccacacagct aatgatgccg cacggaacgc
    81841 caagacgtgt cacctggtcg ataaaaatag cgggcgcacc actgggataa gggccgttcc
    81901 agatacctgg ttgatcaaat ggctggccga ttttactcgc cataatttca accagaattt
    81961 ctccaattgt gaagacctta ctcatatgat taattccttg aaatgattag ctgcgttttc
    82021 ttttatttct tacgtcaaaa taaacggcta caagaatgac taatccttta accaccatct
    82081 ggtaaaaata gggaacattc attaagttca tgccattatt aataacgccg atagttaatg
    82141 cgccgatcac cgtccccacc acggttccat agccgccgtt caggctggtg cctcccagta
    82201 cagccgcagc aatagcatcc agttcgtagc tcaacgcgga attcggttgc ccggaataga
    82261 ggcgtgaggc gaggatcatc ccgctgatac ctgccagcac ggctgtaatc ataaagactt
    82321 taattttcaa gcgggtgaca tttatccctg aataaattgc cgcttcttta ttaccaccag
    82381 tgatataaat ttttcgtcca aaggttgttt tagtaagaat gaagtggtta atcagcaata
    82441 agatgattaa tatccagatt ggaataggta tatagagaaa ttctccattc cccagtgcca
    82501 gaaaggcatc atctttaatc atgatgggca taccatcggt cactatataa gcgataccac
    82561 ggaagatccc catagtagca acggtgacaa taaacgacgg gatccccgct atagccgtca
    82621 agccaccatt tatcatcccc agagccagtg ctgcaataat ggataatggg accaataaaa
    82681 taaatggaat tccccatgaa gccccaagcg cagcaaatgt gccaatcaat gcaatggttg
    82741 aaccgaccga taaatcaata tcgccaagca aaagtacata agtcattcca taggcggtaa
    82801 ttgcaattat cgaaacctga agaataatat tagtaatgtt gttggcggta aaaaaactgt
    82861 cattaataaa actgaacaga atgattaaca ccaccattcc actgaggata ccgccatatt
    82921 gcctgaagac ttttttaaaa ttatttgcaa tgggaatttc cgaaggtttc ggactggcaa
    82981 gagttttctc tgtcatttaa cgtactcctg ttgcagcggt cattacctgt gtctgcgtta
    83041 aattttcaca tgaataaata tcggcaatcg ttttattacg cattaccacc agacgatcgc
    83101 taagtgccat gacttctggc agttcagagg acaccagaat aattgctgtc cctgccgcag
    83161 ccagttgacg tatcacgtta tagatttcaa attttgcgcc gacatccact ccgcgcgttg
    83221 gctcatcgag caataaaata cgcggagttt tctccagcca cttcgataac acgattttct
    83281 gttgattacc gccgctcagc gctcccactg cctgatgtac agaactcact ttagtgttca
    83341 ttctggcaat tgattgcagc gtcttcgcct cttcttgtcg gtgattgatg agtcccatcc
    83401 gcgaagcgtt atattgaaac gccacatggt gcgtgttcat attgacgtcg tgcatcagca
    83461 ctaacccttc ttccttgcgg ttttcggtca caaaagcaat gccatgatca atagcctgtt
    83521 taggattagc gatcctgact tccttaccat caatcaacac agtcccctga tacgaaacga
    83581 ggccaaagag cgccttcatc acatcggaac gcccggcccc caccaggcca aaaaatccga
    83641 ggacctcccc cggacgcaca gcgaaactga tctctttaaa aagcgaagga tgggaaagat
    83701 tttttacttc cagttttgca ggaacatttt catccggtat ttccccaaga cgtgctggcc
    83761 aaatattttt catttcccga ccaaccattt gggcaatcaa atgctggata tcactgtttt
    83821 tcttttcatg cgtggcgata tactcaccgt cgcggatcac cgaaatatca tctgaaatcc
    83881 gcataatttc atccatgcga tgagagatat aaataaccgc acagcctcgc tgctttaatt
    83941 tttcaataat gttaaacaga atctccgctt cattatcact tagcgaagat gtcggttcat
    84001 ccataatcac gacatccgct gcatagctta aggctttagc aatttctacc agttgcctat
    84061 gtgccatcga catattgccg accatttcta gtgggtctag tggtatgccc agttccgcga
    84121 ggagattctg gcaatctgcg agcatttttt tatcgttaat aatgccaaaa cgtcgcggtt
    84181 cattattggc ataaatattt tccgccacgg tcatattatt actcaggctc aattcctgaa
    84241 aaataatcgc aattcctaac tggcgtgaat gctgcggatt attaatggtg atagtttcac
    84301 cacgtagtgt tatcgttccc tgggttggcg gataaacacc ggccagtatt ttcattaatg
    84361 tcgatttacc ggcaccattc tcgcctaata aagtatgcac ccgcccttta cgtaaagaga
    84421 gggtaatatc ttttaatgct tgaacatgac caaatgtctt cgcaatatgc gaaacatcaa
    84481 gaataacatc cgtcataatg acctcggtgt cacaacaaaa cgccaataac gttttgttgt
    84541 gaggtgaaga tgtttactat ttacctgtat aaacgccggg ttgtaccggg atcatttttt
    84601 ctaccggttt accattgagg tgatcaacag cggcatcaat ggctttagca cccatttgat
    84661 cgggatattg ccgaatcacg gccgcaaacg ttttttcact gtcaacggca tcacgcgctt
    84721 ctttcatgcc gtcaaaacca atgattttga tgttgtcgtt atgcgcagat tttgctgcga
    84781 taaccgctgc cagcgccgca tcatcaccaa agccaaacag accattcagg ttggcatttg
    84841 cctgcataat gttttgcgca gtcgttaacg cttcagcccg cgtaatacca ggctgttcag
    84901 caacaatttt aatatccggt gtatcgctta accctttctt aaaaccatca acgcgcgcaa
    84961 ccaccgattg tagtgccgga taagaaataa tggctacagt acctttacca ttcaaaagtt
    85021 tgcccattaa cttgcctgct tcaacgcctc ccgcatagtt atcggtcgca acatgcgata
    85081 cgaccggaac gccttccgca gcgacgtcga cggtgataac gggaataccg gccttatcgg
    85141 ctttaattat cgccgcctgg acgcctttcg aatcgaccgg agagataata atggcatcca
    85201 cttttttagt aataaaatcc tcaacatcag aaagttgttt gtttaaatcc tgattggcaa
    85261 tagagacact taaattgaca ttgtcttttt ttgcctcttc tttcatggca ttcgcaagat
    85321 caatatagaa aggatgttgc tgtgttaata atgacgcacc aattttatac tcttttgcca
    85381 acgaattttg cgagaacaat gccaagctca ccaacatagc tgaaccaagt aatttaatat
    85441 ttttcataaa caatcctcat tgtttttgta gggtacaacg tcttaaattt ttccttcact
    85501 accacagaca ccaataatac gagttacgac ttctttcatg gcggcctttg cgggctgcat
    85561 atagtggcgt ggatcatttg ccgcaggatt gttgtagaaa tatcctttta acgcatcggc
    85621 aaacgcaatt ttcaattctg tcgcgacatt cactttacaa ataccgcggc gaatacagtg
    85681 ctgaacgtcc tgttctgata agcccgaagc accatgcaac accagtggaa tctccactcg
    85741 ctgacgaatc tgttccagcc gcacaaaatc cagtcgtggt tcatgcgcat ataaaccatg
    85801 tgctgtgccg attgctacgg ccagggagtc aatttgggta cgttgaacaa actctgcagc
    85861 tgcggctggt gaggtataag cggaatcttt ttcatccacc agcagatcgt cctcctggcc
    85921 gccaagacgc cctagctcgg cctcaacgct aacatcaaag cgatggcaaa aggtggtaac
    85981 ttcttgcact cgcgcaatgt tgtcttcaaa tggcaaatgg gagccatcaa tcatgacaga
    86041 acggatccct gcgcgaacct tgttaaaaat gtcatcactc tcttcatgat ggtcgagatg
    86101 caggactaac ggcaggttgt actcgcgagc cagcgattgt actaacgaaa ttaaacttcc
    86161 cacgccacca taacgataag taccgggtgt tcccgccagc ataacgggag agcgaagttg
    86221 tgcagcgctc tcaacaacta cctgtaaagt ttcaaggtta tgaatgttga aagcggggac
    86281 ggcatatccc tctgactggg cttttttcag catgtttttg cttgaaatga tgtacatcgc
    86341 agctcctgtt atttgtcgtt cgccttacaa aatgaagaaa gagaatattc actttcattt
    86401 tgaaagcaaa ctagcacagc a
//
LOCUS       DAZGTR010000007        83935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGTR010000007 DAZGTR010000000
VERSION     DAZGTR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.007"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            119..562
                     /gene="cmtB"
                     /locus_tag="ACHLCY_000539"
     CDS             119..562
                     /gene="cmtB"
                     /locus_tag="ACHLCY_000539"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP0367859.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            590..1978
                     /gene="cmtA"
                     /locus_tag="ACHLCY_000540"
     CDS             590..1978
                     /gene="cmtA"
                     /locus_tag="ACHLCY_000540"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP0367860.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1993..3270
                     /gene="yggP"
                     /locus_tag="ACHLCY_000541"
     CDS             1993..3270
                     /gene="yggP"
                     /locus_tag="ACHLCY_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0367861.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3267..4232
                     /gene="glpX"
                     /locus_tag="ACHLCY_000542"
     CDS             3267..4232
                     /gene="glpX"
                     /locus_tag="ACHLCY_000542"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0367862.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4254..4763
                     /gene="fumE"
                     /locus_tag="ACHLCY_000543"
     CDS             4254..4763
                     /gene="fumE"
                     /locus_tag="ACHLCY_000543"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP0367863.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4760..5473
                     /locus_tag="ACHLCY_000544"
     CDS             4760..5473
                     /locus_tag="ACHLCY_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP0367864.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5445..6122)
                     /locus_tag="ACHLCY_000545"
     CDS             complement(5445..6122)
                     /locus_tag="ACHLCY_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0367865.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6116..6793)
                     /locus_tag="ACHLCY_000546"
     CDS             complement(6116..6793)
                     /locus_tag="ACHLCY_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0367866.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6781..7488)
                     /locus_tag="ACHLCY_000547"
     CDS             complement(6781..7488)
                     /locus_tag="ACHLCY_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP0367867.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRTFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7489..8067)
                     /locus_tag="ACHLCY_000548"
     CDS             complement(7489..8067)
                     /locus_tag="ACHLCY_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP0367868.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8090..8521)
                     /locus_tag="ACHLCY_000549"
     CDS             complement(8090..8521)
                     /locus_tag="ACHLCY_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP0367869.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8893..9912
                     /gene="epd"
                     /locus_tag="ACHLCY_000550"
     CDS             8893..9912
                     /gene="epd"
                     /locus_tag="ACHLCY_000550"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP0367870.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9962..11125
                     /gene="pgk"
                     /locus_tag="ACHLCY_000551"
     CDS             9962..11125
                     /gene="pgk"
                     /locus_tag="ACHLCY_000551"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP0367871.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11340..12419
                     /gene="fbaA"
                     /locus_tag="ACHLCY_000552"
     CDS             11340..12419
                     /gene="fbaA"
                     /locus_tag="ACHLCY_000552"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP0367872.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12778..13638
                     /gene="mscS"
                     /locus_tag="ACHLCY_000553"
     CDS             12778..13638
                     /gene="mscS"
                     /locus_tag="ACHLCY_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0367873.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13777..14412
                     /gene="argO"
                     /locus_tag="ACHLCY_000554"
     CDS             13777..14412
                     /gene="argO"
                     /locus_tag="ACHLCY_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP0367874.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14505..15245
                     /gene="yggE"
                     /locus_tag="ACHLCY_000555"
     CDS             14505..15245
                     /gene="yggE"
                     /locus_tag="ACHLCY_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP0367875.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15412..16308
                     /gene="ygfI"
                     /locus_tag="ACHLCY_000556"
     CDS             15412..16308
                     /gene="ygfI"
                     /locus_tag="ACHLCY_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0367876.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16305..17783)
                     /gene="scpC"
                     /locus_tag="ACHLCY_000557"
     CDS             complement(16305..17783)
                     /gene="scpC"
                     /locus_tag="ACHLCY_000557"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP0367877.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRASYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17807..18592)
                     /gene="scpB"
                     /locus_tag="ACHLCY_000558"
     CDS             complement(17807..18592)
                     /gene="scpB"
                     /locus_tag="ACHLCY_000558"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP0367878.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18603..19598)
                     /gene="meaB"
                     /locus_tag="ACHLCY_000559"
     CDS             complement(18603..19598)
                     /gene="meaB"
                     /locus_tag="ACHLCY_000559"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP0367879.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19591..21735)
                     /gene="scpA"
                     /locus_tag="ACHLCY_000560"
     CDS             complement(19591..21735)
                     /gene="scpA"
                     /locus_tag="ACHLCY_000560"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP0367880.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21939..22832)
                     /gene="argP"
                     /locus_tag="ACHLCY_000561"
     CDS             complement(21939..22832)
                     /gene="argP"
                     /locus_tag="ACHLCY_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP0367881.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22974..23159
                     /locus_tag="ACHLCY_000562"
     CDS             22974..23159
                     /locus_tag="ACHLCY_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367882.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23215..23874
                     /gene="rpiA"
                     /locus_tag="ACHLCY_000563"
     CDS             23215..23874
                     /gene="rpiA"
                     /locus_tag="ACHLCY_000563"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP0367883.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24130..25362
                     /gene="serA"
                     /locus_tag="ACHLCY_000564"
     CDS             24130..25362
                     /gene="serA"
                     /locus_tag="ACHLCY_000564"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0367884.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25591..25650
                     /gene="ibsC"
                     /locus_tag="ACHLCY_000565"
     CDS             25591..25650
                     /gene="ibsC"
                     /locus_tag="ACHLCY_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP0367885.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25983..26042
                     /locus_tag="ACHLCY_000566"
     CDS             25983..26042
                     /locus_tag="ACHLCY_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0367886.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26143..26745)
                     /gene="fau"
                     /locus_tag="ACHLCY_000567"
     CDS             complement(26143..26745)
                     /gene="fau"
                     /locus_tag="ACHLCY_000567"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP0367887.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26766..26949)
                     /gene="ssrS"
                     /locus_tag="ACHLCY_000568"
     ncRNA           complement(26766..26949)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHLCY_000568"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26991..27320)
                     /gene="zapA"
                     /locus_tag="ACHLCY_000569"
     CDS             complement(26991..27320)
                     /gene="zapA"
                     /locus_tag="ACHLCY_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0367888.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27488..28066
                     /gene="ygfB"
                     /locus_tag="ACHLCY_000570"
     CDS             27488..28066
                     /gene="ygfB"
                     /locus_tag="ACHLCY_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP0367889.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28092..29417
                     /gene="pepP"
                     /locus_tag="ACHLCY_000571"
     CDS             28092..29417
                     /gene="pepP"
                     /locus_tag="ACHLCY_000571"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP0367890.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29414..30592
                     /gene="ubiH"
                     /locus_tag="ACHLCY_000572"
                     /gene_synonym="visB"
     CDS             29414..30592
                     /gene="ubiH"
                     /locus_tag="ACHLCY_000572"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP0367891.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30615..31817
                     /gene="ubiI"
                     /locus_tag="ACHLCY_000573"
                     /gene_synonym="visC"
     CDS             30615..31817
                     /gene="ubiI"
                     /locus_tag="ACHLCY_000573"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP0367892.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31891..31974)
                     /locus_tag="ACHLCY_000574"
     CDS             complement(31891..31974)
                     /locus_tag="ACHLCY_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367893.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32265..33359
                     /gene="gcvT"
                     /locus_tag="ACHLCY_000575"
     CDS             32265..33359
                     /gene="gcvT"
                     /locus_tag="ACHLCY_000575"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP0367894.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33383..33772
                     /gene="gcvH"
                     /locus_tag="ACHLCY_000576"
     CDS             33383..33772
                     /gene="gcvH"
                     /locus_tag="ACHLCY_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP0367895.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33891..36764
                     /gene="gcvP"
                     /locus_tag="ACHLCY_000577"
     CDS             33891..36764
                     /gene="gcvP"
                     /locus_tag="ACHLCY_000577"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP0367896.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            37025..37768
                     /gene="ygfF"
                     /locus_tag="ACHLCY_000578"
     CDS             37025..37768
                     /gene="ygfF"
                     /locus_tag="ACHLCY_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0367897.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37825..39258)
                     /gene="bglA"
                     /locus_tag="ACHLCY_000579"
     CDS             complement(37825..39258)
                     /gene="bglA"
                     /locus_tag="ACHLCY_000579"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP0367898.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            39303..39614
                     /gene="yqfB"
                     /locus_tag="ACHLCY_000580"
     CDS             39303..39614
                     /gene="yqfB"
                     /locus_tag="ACHLCY_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP0367899.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39778..40437
                     /gene="yqfA"
                     /locus_tag="ACHLCY_000581"
     CDS             39778..40437
                     /gene="yqfA"
                     /locus_tag="ACHLCY_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP0367900.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40633..41613)
                     /gene="ygfZ"
                     /locus_tag="ACHLCY_000582"
     CDS             complement(40633..41613)
                     /gene="ygfZ"
                     /locus_tag="ACHLCY_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP0367901.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41856..42122
                     /gene="sdhE"
                     /locus_tag="ACHLCY_000583"
     CDS             41856..42122
                     /gene="sdhE"
                     /locus_tag="ACHLCY_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP0367902.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            42103..42510
                     /gene="ygfX"
                     /locus_tag="ACHLCY_000584"
     CDS             42103..42510
                     /gene="ygfX"
                     /locus_tag="ACHLCY_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP0367903.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42550..43071)
                     /gene="fldB"
                     /locus_tag="ACHLCY_000585"
     CDS             complement(42550..43071)
                     /gene="fldB"
                     /locus_tag="ACHLCY_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP0367904.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43183..44079
                     /gene="xerD"
                     /locus_tag="ACHLCY_000586"
     CDS             43183..44079
                     /gene="xerD"
                     /locus_tag="ACHLCY_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP0367905.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            44104..44814
                     /gene="dsbC"
                     /locus_tag="ACHLCY_000587"
     CDS             44104..44814
                     /gene="dsbC"
                     /locus_tag="ACHLCY_000587"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP0367906.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44820..46553
                     /gene="recJ"
                     /locus_tag="ACHLCY_000588"
     CDS             44820..46553
                     /gene="recJ"
                     /locus_tag="ACHLCY_000588"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP0367907.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46644..47742
                     /gene="prfB"
                     /locus_tag="ACHLCY_000589"
     CDS             join(46644..46718,46720..47742)
                     /gene="prfB"
                     /locus_tag="ACHLCY_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP0367908.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47752..49269
                     /gene="lysS"
                     /locus_tag="ACHLCY_000590"
     CDS             47752..49269
                     /gene="lysS"
                     /locus_tag="ACHLCY_000590"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0367909.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49312..49860)
                     /gene="idi"
                     /locus_tag="ACHLCY_000591"
     CDS             complement(49312..49860)
                     /gene="idi"
                     /locus_tag="ACHLCY_000591"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP0367910.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHRAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            49915..49986
                     /locus_tag="ACHLCY_000592"
     CDS             49915..49986
                     /locus_tag="ACHLCY_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367911.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(49983..50108)
                     /gene="yqfG"
                     /locus_tag="ACHLCY_000593"
     CDS             complement(49983..50108)
                     /gene="yqfG"
                     /locus_tag="ACHLCY_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP0367912.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50110..51558)
                     /gene="uacT"
                     /locus_tag="ACHLCY_000594"
     CDS             complement(50110..51558)
                     /gene="uacT"
                     /locus_tag="ACHLCY_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP0367913.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51994..53913
                     /gene="ygfT"
                     /locus_tag="ACHLCY_000595"
     CDS             51994..53913
                     /gene="ygfT"
                     /locus_tag="ACHLCY_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP0367914.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53913..54401
                     /gene="ygfS"
                     /locus_tag="ACHLCY_000596"
     CDS             53913..54401
                     /gene="ygfS"
                     /locus_tag="ACHLCY_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0367915.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(54437..55804)
                     /gene="ghxQ"
                     /locus_tag="ACHLCY_000597"
                     /gene_synonym="ygfQ"
     CDS             complement(54437..55804)
                     /gene="ghxQ"
                     /locus_tag="ACHLCY_000597"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP0367916.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55840..57156)
                     /gene="guaD"
                     /locus_tag="ACHLCY_000598"
     CDS             complement(55840..57156)
                     /gene="guaD"
                     /locus_tag="ACHLCY_000598"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP0367917.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57174..58574)
                     /gene="xanQ"
                     /locus_tag="ACHLCY_000599"
     CDS             complement(57174..58574)
                     /gene="xanQ"
                     /locus_tag="ACHLCY_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP0367918.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(58739..61609)
                     /gene="xdhD"
                     /locus_tag="ACHLCY_000600"
     CDS             complement(58739..61609)
                     /gene="xdhD"
                     /locus_tag="ACHLCY_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP0367919.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYNAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(61606..62385)
                     /gene="ygfM"
                     /locus_tag="ACHLCY_000601"
     CDS             complement(61606..62385)
                     /gene="ygfM"
                     /locus_tag="ACHLCY_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP0367920.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(62436..63764)
                     /gene="ssnA"
                     /locus_tag="ACHLCY_000602"
     CDS             complement(62436..63764)
                     /gene="ssnA"
                     /locus_tag="ACHLCY_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP0367921.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(63767..66865)
                     /gene="ygfK"
                     /locus_tag="ACHLCY_000603"
     CDS             complement(63767..66865)
                     /gene="ygfK"
                     /locus_tag="ACHLCY_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP0367922.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLAYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(67187..67765)
                     /gene="mocA"
                     /locus_tag="ACHLCY_000604"
     CDS             complement(67187..67765)
                     /gene="mocA"
                     /locus_tag="ACHLCY_000604"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP0367923.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            67868..68638
                     /gene="yqeC"
                     /locus_tag="ACHLCY_000605"
     CDS             67868..68638
                     /gene="yqeC"
                     /locus_tag="ACHLCY_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP0367924.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            68686..70311
                     /gene="yqeB"
                     /locus_tag="ACHLCY_000606"
     CDS             68686..70311
                     /gene="yqeB"
                     /locus_tag="ACHLCY_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP0367925.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(70352..71284)
                     /gene="arcC"
                     /locus_tag="ACHLCY_000607"
     CDS             complement(70352..71284)
                     /gene="arcC"
                     /locus_tag="ACHLCY_000607"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0367926.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(71332..72717)
                     /gene="hyuA"
                     /locus_tag="ACHLCY_000608"
     CDS             complement(71332..72717)
                     /gene="hyuA"
                     /locus_tag="ACHLCY_000608"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP0367927.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(72770..73981)
                     /gene="ygeY"
                     /locus_tag="ACHLCY_000609"
     CDS             complement(72770..73981)
                     /gene="ygeY"
                     /locus_tag="ACHLCY_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP0367928.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(74039..75235)
                     /gene="dpaL"
                     /locus_tag="ACHLCY_000610"
     CDS             complement(74039..75235)
                     /gene="dpaL"
                     /locus_tag="ACHLCY_000610"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP0367929.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(75293..76480)
                     /gene="ygeW"
                     /locus_tag="ACHLCY_000611"
     CDS             complement(75293..76480)
                     /gene="ygeW"
                     /locus_tag="ACHLCY_000611"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP0367930.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            76959..78737
                     /gene="ygeV"
                     /locus_tag="ACHLCY_000612"
     CDS             76959..78737
                     /gene="ygeV"
                     /locus_tag="ACHLCY_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP0367931.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAFGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(78777..79256)
                     /gene="xdhC"
                     /locus_tag="ACHLCY_000613"
     CDS             complement(78777..79256)
                     /gene="xdhC"
                     /locus_tag="ACHLCY_000613"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP0367932.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(79253..80131)
                     /gene="xdhB"
                     /locus_tag="ACHLCY_000614"
     CDS             complement(79253..80131)
                     /gene="xdhB"
                     /locus_tag="ACHLCY_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP0367933.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(80142..82439)
                     /gene="xdhA"
                     /locus_tag="ACHLCY_000615"
     CDS             complement(80142..82439)
                     /gene="xdhA"
                     /locus_tag="ACHLCY_000615"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP0367934.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            82854..83609
                     /gene="actS"
                     /locus_tag="ACHLCY_000616"
     CDS             82854..83609
                     /gene="actS"
                     /locus_tag="ACHLCY_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP0367935.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            83688..83761
                     /locus_tag="ACHLCY_000617"
     tRNA            83688..83761
                     /locus_tag="ACHLCY_000617"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83720..83722,aa:Gly,seq:ccc)
BASE COUNT        20333 a        20890 c        22233 g        20479 t
ORIGIN      
        1 attaataatt cactgttttc aaaacatcgg tttccctgct caattgcttt cattaaaccg
       61 cacgagtatt ttatttttac cgccggacgg taccctaaaa ataaaataag gataatttat
      121 gcggcttagt gattattttc cagaatcatc aatctcagtt atacattcag caaaagattg
      181 gcaggaagct atcgatttct cgatggtatc attgctggat aaaaactata tcagcgagaa
      241 ttacattcag gcaattaaag attccaccat taacaatggc ccttattata ttctcgcacc
      301 aggcgtggca atgcctcatg cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt
      361 gacattactt gaacaaggtg tttattttcc ggggaatgac gaaccaatta aattactcat
      421 cggactctct gctgccgatg ccgattcgca cattggcgct attcaggcgt taagtgaatt
      481 actgtgcgaa gaagaaatac tcgaacaact cttaacagca tcatcagaaa aacaattagc
      541 ggacattatc agccgcggat aattactctc tcctctacag gataatatta tggaaaacaa
      601 gtctgctcgt gcaaaggtcc aggcttttgg gggctttttg actgcaatgg tcatccccaa
      661 tattggtgct tttattgcct ggggttttat tactgcgtta tttattccca ccggttggct
      721 gcctaacgaa catttcgcca aaattgtcgg cccgatgatt acctatttat tgcccgtgat
      781 gattggttct acaggtggtc atctggtcgg cggtaaacgc ggggcggtca tgggcggaat
      841 aggtactatt ggtgtgatcg ttggcgcaga gatcccgatg ttccttggct caatgatcat
      901 ggggccgctc ggtgggttgg tcataaaata tgtcgataag gcactggaaa aacgcatacc
      961 tgccggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga tgctcctttg
     1021 tctgctgggt tttgaagtga tcggcccggc ggtgttaatt gccaatactt tcgtcaaaga
     1081 gtgtatcgag gcgctggtac atgcgggtta tctgcctctg ctgtcagtca tcaatgaacc
     1141 ggcgaaagtg cttttcctta ataatgcgat cgatcagggc gtctattatc cgctgggaat
     1201 gcaacaggct tcggttaacg gtaaatccat cttctttatg gtggcctcta acccaggtcc
     1261 gggcctgggg ctgctgctgg cgtttacctt gtttggtaaa gggatgagta aacgttctgc
     1321 gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt tcccatatgt
     1381 gctgatgaag ccgctgacta ttattgccat gattgcgggc ggtatgtctg gtacctggat
     1441 gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta tctttgctta
     1501 cctggcactg acgccgaaag gctcgttcct ggcgacaatt gccggtgtta cggtaggtac
     1561 cctggtgtcc tttgctatta cttcgctgat actgaagatg gaaaaaacgg tggaaacgga
     1621 gagcgaagat gagtttgctc agtcagccaa tgcggttaag gcgatgaaac aagagggtgc
     1681 attctcgtta agcagggtta agcgtatcgc ctttgtttgc gatgcgggga tgggctccag
     1741 tgcgatgggc gcgaccacct tccgtaaacg cctggaaaaa gcggggctgg caatagaagt
     1801 aaaacattac gcgatagaaa acgtgcctgc ggatgccgat atcgtcgtca ctcacgccag
     1861 tctggaaggg cgcgtgaaac gtgtgacgga taaaccgctg atattgatta ataactatat
     1921 tggcgatcca aaactcgaca ctttatttaa tcaattaacc gccgaacata aacactgatt
     1981 acagaggtaa aaatgaaaac caaagttgct gctatttatg gcaagcggga tgtccgtctg
     2041 cgcgaatttg aactgccaga aattaccgat aatgaactgt tagtgagtgt aatttctgac
     2101 agcgtctgtt tatcgacctg gaaagcggcg ttactcggta gtgaacataa acgcgtaccc
     2161 gacgatttag aaaatcatcc ggtcattacc gggcatgaat gtgccggggt tattgtcgaa
     2221 gtgggtaaaa atctcactgg caaatataaa aaaggccagc gttttgtatt gcaaccggcg
     2281 atggggttac caagcggata ttcagcaggc tacagctacg aatattttgg cggtaacgcc
     2341 acctatatga ttattcccga aatcgccatt aatttgggct gcgtattacc gtatcacggc
     2401 tcttattttg ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat
     2461 gccaattatc acaccacgca atatgtttat gagcatcgca tgggcgtcaa acctggcggc
     2521 aatattgcac tgctggcgtg tgcgggtccg atgggcattg gcgctatcga ttacgccatt
     2581 aacggtggca tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa acgcctggcg
     2641 caggtgcaga agctgctgcc ggtggatctg gcggccagta aaggcattga gctggtgtat
     2701 gtgaatacca aagggatgag cgatccggtc cagacgctgc gggcgctgac aggagatgtc
     2761 gggttcgatg acatttttgt ttatgcggcg gtgcctgctg tcgttgagat ggctgacgaa
     2821 ttactggcgg aagatggctg tctgaacttc tttgccgggc cgacggataa aaacttcaaa
     2881 gtgccgttta atttctacaa cgtccattac aacagcacgc acgtagtcgg tacatccggc
     2941 ggttcaacgg acgacatgaa agaggcgatt gcgttaagcg ccacggggca gttgcagccg
     3001 tcctttatgg tcacgcatat cggtgggctg gatgcggtgc cagataccgt gctcaatctg
     3061 ccggatatcc ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctcactgcc
     3121 attgccgatt ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagtt ggcgcggctg
     3181 gttgaggaaa cgcacggcat ctggaatgaa caggccgaga aatatctgct ggcacaattt
     3241 ggcgttgata tcggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta
     3301 cggaacaggc agcgttggct gcctggccgc aaaccggatg tggcgacaaa aataaaattg
     3361 atggcctggc ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt cgtggacgag
     3421 tggttatcgg cgaaggagaa attgaccatg cgccgatgct gtggattggc gaagaggtgg
     3481 gcaaaggcga tgggccagaa gtcgatatcg cggttgaccc gattgaaggc acgcggatgg
     3541 tagcgatggg gcagagcaat gcgctggcgg tcatggcttt cgcccctcga gatagcctgt
     3601 tacatgcgcc cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga
     3661 ttgatctgtc tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac
     3721 cgctggacaa gctgcgcatg gttacgctgg ataaaccgcg cctgagtgcc gcgattgaag
     3781 aagcaacaca gctgggcgtg aaggttttcg ctctgccgga tggcgatgtc gccgccagcg
     3841 tgctgacctg ctggcaggat aatccttacg acgtgatgta caccatcggc ggtgcgccag
     3901 aaggcgtgat ttctgcctgt gccgttaaag cattaggtgg ggatatgcag gccgagctga
     3961 ttgacttttg tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc
     4021 gcaagcgttg taaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg
     4081 tgagggggaa cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg
     4141 gtatccaaca gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc
     4201 aaacgtgtaa tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga
     4261 cgctgacaga agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta
     4321 tgaaaactgc gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg
     4381 tcgataacga tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc
     4441 cgcttgacga tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat
     4501 ggctgtatgc cgatatcacg cacttttccc agtactggca ttacctgaac gaacaggatg
     4561 aaacgcccgg atttgccgat gacatgacct gggattttat cagcaacgtc aatagcataa
     4621 cctgcaatgc gacgctctat gacgcgttaa aagcgatgaa gttcgccgat ttcgccgtct
     4681 ggtcagaggc gcgttttagc ggaatggtca aaacggcgct gacgctggca gtaacgacca
     4741 ccttaaagga attaacgccg tgaaaattga attaacggtg aatgggctga acgtgcaagc
     4801 gcagtaccat gatgaagaga tagaacgcgt gcataaaccg ttgctgcgta tgctggcggc
     4861 cttacagacg gtaagtccgc agcgacgtac ggtggttttt ctttgcgctc cgccggggac
     4921 gggtaaatct accttaacta ccttctggga atatctcgcg caacaagacc ctgaattacc
     4981 cgctatccag acgctcccga tggatggttt tcaccattac aatagctggc tggatgcgca
     5041 tcaattgcgc cccttcaaag gcgcaccaga gacatttgac gttgcgaaac tggcggaaaa
     5101 tctgtgccag gtagtggaag gggattgtac gtggccgcag tatgatcgac aaaagcatga
     5161 tcctgttgaa gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt
     5221 gttgctggac gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat
     5281 caacgcgcca gctactgcgt tgcgggaacg actggtaggg cgcaagctgg cgggaggggt
     5341 atcgctggca gacgcagaag ctttttatga ccgtaccgac gggccgaatg ttcgccgggg
     5401 gctggaagag agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg
     5461 tctggtggat taacgttcct gattagacca tcttccagtc gcaccacacg agagaaatga
     5521 cgccgggtaa atgcggcgtc atgactgatt gctacgacgc tcgtgcctcg ctggccgcac
     5581 ttctccagcc agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg
     5641 tccagcagca atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc
     5701 tgcgcactat gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg acaacattgc
     5761 agggcagcat gggtacgttg agtgatttca tccgccgggc atttttgcag tttcaaacca
     5821 aatgcgatct cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc ctgaaacagt
     5881 acgccaacct ttgccgcgcg ctggcggttt ttcaggtttt tcatcgcctg ttgttgcagc
     5941 ataaccgtac cggctgtggg cgtaagaaga cctgccatta ctcgtaacag cgtcgatttt
     6001 cccgcgccat tgtcgccggt cagcgccaac cattcacctt gtttgagttg cagagaaatg
     6061 tcgcaaagac aatccgttgc cgcgccaggc cagcgataag atatcttatt taacgttaac
     6121 atagtggctg gagcgctccg ttttgcaatt gccacgcttg ctggcagtag ttcaggaaag
     6181 gtgtgcgatg acgttcaaaa agaataatca gcgaaccacg ttcaaacgcc cagtgttgta
     6241 agcgctgcaa cagcatttcg ctggcctgcg gagtcaggcg gctaaaggct tcatcaagaa
     6301 ttaagagctt tggctgcatc gcgatggcgc aggcgatgac cacgcgctgt gtctctccac
     6361 cggagagtgt tgccgggtga cgatggcgca acggctggca ctcggtcaga gtcagggcgg
     6421 catcaatccg cgccataatc tctgcttcag caagacacag attttctggc ccaaaggcaa
     6481 cttcttcttc cacgctaaag gtacagccgg agagctgcaa atagggcgat tgctggacga
     6541 gctggatggt ggcggattgt tcattaagcg ggagatgccc aatgggcgta ccgaggagcg
     6601 tccccgtgcc tgttatctct cctggcagaa agtccggata ccagcccgcc atcagctgtg
     6661 ccagcgtact tttgccactg ccgttgtcac caaagatggc gaccatcccc ggtttgacat
     6721 agtgaaagtc ataacagaac ggaggatgcg ttgagtgtgt cgggcaataa cggaactgtt
     6781 ctaacgtaac catatccacg ctccgaattc tgtcagcatc agcaagatca tggtgtaacg
     6841 cgctacgcgt tgtagggtgc tatcggctgg tgcccacaac gttgttcgtc ggttgtggat
     6901 ccgaaatgtc cgcatatcca gcgccgcacc acggacagcc agatcgttaa gcgcattgtg
     6961 tgttagcgga ataattaacg cgggcatcgc acgtaaacgc tgataccagc cctcatccag
     7021 cggcacgccg cgggcacgct gcgcttcatg aataatcgct aattgccgtt ttaactgctc
     7081 aacgaccaat aacggcccgg caaacagata agcgacgcct ggcggcaggc gagaagcaaa
     7141 cagagcgcga ataaaacatt gcacggggac gtactgcatc cacaactgtg aggttgaaac
     7201 aatcgccaga atccgcaacc agagcgtaat agcatgcgac caccgttctg gcgagcgtgg
     7261 cgtgccgctt agccattctg gtagccagcc accgtgtacc agccacagcc cggctcccag
     7321 cgagaacatc agccagacga catattttgc ccgccgccgc gtggccttta gcgcaataag
     7381 gcagaaaaag gtcgccgcgc tatagatcgg caaaatagtc tgcgcgggca ggataagcgt
     7441 ggtgcaggcc gccaatgccc acagtgttaa tgaggtaaat ggatgcattt agcgcacggc
     7501 agccattgcc ggaaaatggc gcgtggtgcg aatcggcagc tggcgcagta aaagccagac
     7561 aatcaccgct gtaagaattt tatcgaccag gttcgcgcct atcaccgtaa tcgccaccga
     7621 ctccaccagg ttttgcccca tcgagtgcat ccaggcaacg aacaaatcgg ctccgctacc
     7681 ggtgacaccg ccgaacagcg cagtacgcag cggtacagcg acgaccgtta ccgcaagcgt
     7741 aataatcacg ccgctgacaa cgacttttgg cagggtgcga aaccaacccg ctcgcgccag
     7801 gcaaccggcg accaggccga tgaccatcgc gacgggtgca aatgcggcgg cgatcgggtt
     7861 ggtcagcaat ccccagagta aattggtcag taagccggtc agcattccga tcaccggacc
     7921 aagcagtacc gcgctaataa gtgtgccgat ggagtcgaga aatataggta atttgaccat
     7981 gctggccagt tgcccgccga tcatattgat ggcgatagat ataacgatca acaccagtgc
     8041 ctgactggaa aagtggctac gcgccataag aaaacctctt tgaaagtagt tatttaacgg
     8101 gggaaatatg caactcgtcg taaccagaaa gcgcgcaagg ttggcggcta aacgccagtt
     8161 cctcaaggct accgatcacc agttcaagcg tggtccgcac ggtacagccg ggcatcatat
     8221 gaccgccaag catcgtgccg tgtggatcgg aaacgcagag atgaagatgc tcaccactct
     8281 gttccagcgt gccgtttagt gagatcactt cgaatgtacc gttcaggagc gtggtgcctt
     8341 cttgcccggc atagcgcaag gcgacgtcag tcaggctacc ggtacagcct gcaatccagg
     8401 cggcatgaag ttgatgctgc tgtgcaaaag cgtgtagctg ggaaaaaact tcctgcccgg
     8461 ggaggaggcg cagtgcgtaa aagcgtgcgg aggaagaata tgggtagggc aatgtcatca
     8521 ttggagtact ccgaaactcg gacgtattgc ggataatatc gacgctgcat cagtcatatt
     8581 ctgatataag tcagggtttg tgacggcaca acgaaaagat tttgctacgc ttattcgtga
     8641 ttctgcaaag tggaataaat tccacagggg cggggctgaa aacgtgacgt aagtcagata
     8701 attgctttct ttgactggac aatcattcct tttattccac gtttcgctta tcctagctga
     8761 agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt gcgacaggta
     8821 aggtttcccc ggacgatttg ctggattact ctgtctggca aatttgctca gggaaaacct
     8881 tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat cgggcgtaat
     8941 gtggttcgtg ctttgtatga atccggacgc cgggcggaaa ttaccgtggt ggcaatcaac
     9001 gaactggcgg atgctgcggg catggcgcat ttgttgaaat atgacaccag ccatggccgt
     9061 tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga cgccatccgc
     9121 gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt tgatgtagtc
     9181 ctcgactgca ccggcgtata tggctcccgc gagcatggcg aagcgcatat tgccgccggg
     9241 gccaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac cgttgtttac
     9301 ggcgtcaatc aggatcaact tcgtgcggaa caccgcatcg tttctaacgc ttcctgtacc
     9361 acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat tgagtccggc
     9421 actgtgacca caattcactc cgccatgcac gatcaacagg ttattgatgc ataccatcct
     9481 gacctgcgtc gcacccgggc agccagccag tcgatcattc cggtcgatac taaactggcc
     9541 gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaagcgat tgcggtacgt
     9601 gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa acctgtaaaa
     9661 gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca tggtatagtt
     9721 gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca cagtgccatt
     9781 gtcgatggca cccaaacccg ggtcagtggc gcacacctga tcaaaacgtt ggtctggtgc
     9841 gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat ggctactgtt
     9901 gctttcaggt aagacgcaag cagcgtctgc aaaactttta gaatcaacga gaggattcac
     9961 catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat ttatccgtgc
    10021 ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta tccgtgcttc
    10081 tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa cttcccacct
    10141 gggtcgtcct accgaaggcg agtacaacga agaattctct ctgctgccgg ttgttaacta
    10201 cctgaaagac aaactgtcta acccggttcg cctggttaaa gattacctcg acggcgttga
    10261 cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag gcgagaagaa
    10321 agacgacgaa accctgtcca aaaaatacgc tgcactgtgt gacgtgttcg taatggacgc
    10381 attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat tcgctgacgt
    10441 tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag cactgaaaga
    10501 accagctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca aactgaccgt
    10561 tctggactcc ttgtctaaaa tcgctgacca gctgattgtt ggtggtggta tcgctaacac
    10621 ctttatcgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg acctggttga
    10681 cgaagctaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg ttcgcgtagc
    10741 aaccgagttc tctgaaactg caccggctac cctgaaatct gttaacgatg tgaaagctga
    10801 cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa tcctgaagaa
    10861 tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga acttccgcaa
    10921 aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca tcgctggcgg
    10981 cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct cctacatctc
    11041 cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg cagtagcgat
    11101 gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc ccttgtttca
    11161 gcgcgctttt agagcacgca ttttttcata ctctaaataa ttcgagttgc aggaaggcga
    11221 caagagagtg aatccccagg agcttacata agtaagtgac tggggtgagc gaacgcagac
    11281 gcagcacatg caacttgaag tatgatgagt ataaggcccg acgatacagg acaagagaca
    11341 tgtctaagat ttttgatttc gtaaaacctg gcgtaatcac tggtgatgac gtacagaaag
    11401 ttttccaggt agcaaaagaa aacaacttcg cactgccagc agtaaactgc gtcggtactg
    11461 actccatcaa cgccgtactg gaaactgctg ctaaagttaa agcgccggtt atcgttcagt
    11521 tctccaacgg tggtgcttcc tttatcgctg gtaaaggcgt gaaatctgac gttccgcagg
    11581 gtgctgctat cctgggcgcg atctctggtg cgcatcacgt tcaccagatg gctgaacatt
    11641 atggtgttcc ggttatcctg cacactgacc actgcgcgaa gaaactgctg ccgtggatcg
    11701 acggtctgct ggacgcgggt gaaaaacact tcgcagctac cggtaagccg ctgttctctt
    11761 ctcacatgat cgacctgtct gaagaatctc tgcaagagaa catcgaaatc tgctctaaat
    11821 acctggagcg tatgtccaaa atcggcatga ctctggaaat cgaactgggt tgcaccggtg
    11881 gtgaagaaga cggcgtggac aacagccaca tggacgcttc tgcactgtac acccagccgg
    11941 aagacgttga ttacgcatac accgagctga gcaaaatcag cccgcgtttc accatcgctg
    12001 cgtccttcgg taacgtacac ggtgtttaca agccgggtaa cgtggttctg actccgacca
    12061 tcctgcgtga ttctcaggaa tatgtttcta agaaacacaa cctgccgcac aacagcctga
    12121 acttcgtatt ccacggtggt tccggttcta ctgctcagga aatcaaagac tccgtaagct
    12181 acggcgtagt gaaaatgaac atcgataccg atacccaatg ggcaacctgg gaaggcgttc
    12241 tgaactacta caaagcgaac gaagcttatc tgcagggtca gctgggtaac ccgaaaggcg
    12301 aagatcagcc gaacaagaaa tactacgatc cgcgcgtatg gctgcgtgcc ggtcagactt
    12361 cgatgatcgc tcgtctggaa aaagcattcc aggaactgaa cgcgatcgac gttctgtaag
    12421 tcattccttt ctgcttatct caaggcccgc tctgcgggtc tttttttcgc caaaagcaaa
    12481 attgcctgat gcgctacgct tatcaggatc tacacgtatc ctgcaatata ttgaatttgc
    12541 aggtttttgt aggccggata aggcgttcac gccgcatccg gcatgacaaa gcgcacattg
    12601 atatctgtct gagccccgct ctgcggggat tttttcgcca aaagcaaggt gattcagatg
    12661 agaaatctgt gatctatttg gcaaaattat gctttattgt ttacccttgt cagactgccc
    12721 gtcataaggc ggcggagtgt atttctccat tttgagtcag ttgaaaagga atattgaatg
    12781 gaagatttga atgttgtcga tagcataaac ggcgcgggaa gctggctggt agctaaccag
    12841 gcgctgctgc taagttatgc agtaaacatc gtggcggcac tcgcgatcat catcgttggt
    12901 ttgattatcg cgcggatgat ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc
    12961 gatgccactg ttgctgattt tctttctgca ttagtccgtt acggtattat cgcctttacg
    13021 ctaatcgcag ccctgggacg cgtgggtgta caaacagcgt cagtcattgc tgtactcggt
    13081 gccgcaggct tagctgttgg tctggctttg caggggtcac tttctaacct ggccgctggc
    13141 gtgttacttg tcatgttccg cccgttccgt gccggagaat atgttgacct cggcggcgta
    13201 gccggtactg tgctgagtgt gcagattttc tccaccacca tgcgtactgc agacggtaaa
    13261 attatcgtta ttccgaacgg taaaattatt gccggaaata ttattaactt ctcccgcgag
    13321 ccagttcgcc gtaacgaatt tattattggc gtggcgtatg attccgatat cgatcaggtt
    13381 aagcagatcc tgaccaatat tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg
    13441 actgtgcgcc tgaacgaact tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc
    13501 aacagcggcg atctgcaaaa cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt
    13561 gatgccgccg gtatcagctt cccgtacccg caaatggatg tgaactttaa gcgggtgaaa
    13621 gaagacaaag ctgcgtaatc aacgctgagg ccagataata ctctctggcc tctctcttat
    13681 tagttattct gattgccaat taatattatc aatttccgct aataacaatc ccgcgatata
    13741 gtctctgcat cagatactta attcggaata tccaacgtgt tttcttatta ctttcaaggt
    13801 cttgctcttg gggcagctat gattctgccg ctcggcccac aaaatgcttt tgtgatgaat
    13861 cagggcattc gtcgtcagta ccacattatg attgccttac tttgcgctat cagcgatttg
    13921 gtcctgattt gcgcggggat ttttggtggt agcgcgttat tgatgcagtc gccgtggttg
    13981 ctggcgctgg tcacctgggg cggcgtagtc ttcttgctgt ggtatggttt tggcgctttt
    14041 aaaacagcga tgagtagtaa tattgagtta gctagtgccg aagtcctgaa gcaaggcaga
    14101 tggaaaatta tcgccaccat gttggcagtg acctggctga atccgcatgt ttacctggat
    14161 acttttgttg tactgggcag ccttggcggg cagcttgacg tcgaaccaaa acgttggttt
    14221 gcgctcggga caattagcgc ctctttcctg tggttctttg gtctggctat tctcgcagcc
    14281 tggctggcac cgcgcctgcg cacggcaaaa tcacagcgca ttatcaatct ggtagtggga
    14341 tgtgtcatgt ggtttatagc cttgcagctg gcgagagacg gtattgctca tgcacaagcc
    14401 ttgttcagtt aggcgctatc tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag
    14461 aggtcctgaa ttttgggcaa gtcaattaac acggagagac taacgtgaag ttcaaagtta
    14521 tcgccctggc ggcattaatg ggtattagcg ggatggcagc gcaggctaac gaattgccgg
    14581 atggaccgca tattgtcacc tccggtacgg caagtgtgga tgcggtgcca gacattgcca
    14641 ctcttgcgat tgaagttaac gtggccgcga aggatgccgc tacagccaag aaacaggcag
    14701 atgagcgcgt cgcacaatac atttccttcc ttgaactcaa tcagatcgcg aaaaaagata
    14761 tcagctcagc gaatttacgc acccagccag attatgatta tcaggatggt aaaagtatcc
    14821 tgaaaggcta ccgcgctgtg agaacggtgg aagtcacgct ccgtcagtta gacaaactga
    14881 attccttgct ggatggcgcg ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg
    14941 gcgtggcgca gccggatgcc tataaagaca aagcgcgtaa ggcagcgatt gataacgcga
    15001 ttcatcaggc gcaggaactg gcgaacggct ttcatcgtaa actggggccg gtatatagcg
    15061 tgcgctacca tgtttccaac tatcagccca gcccaatggt gcggatgatg aaagccgatg
    15121 ccgcgccggt gtctgcccag gaaacttacg agcaggccgc tattcagttt gatgatcagg
    15181 tcgatgtggt cttccagtta gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac
    15241 aataatactt gtagcccgac gtattcgtcg ggctaatgcc tttacccgat attgcctcct
    15301 ttttctgtaa ttttctgtaa aaccatgacg taatcaccta tctttgctcg cggtttcatc
    15361 aaggataggc tgactgaaac ggttaagcgg acaaggatgc ggggaggggg gatggacatt
    15421 tttatttcga aaaagatgcg caatttcatt ttattagcgc aaaccaataa tattgcccgg
    15481 gcggcagaaa aaatccatat gacggcttcg ccatttggta aaagcattgc cgcgctggaa
    15541 gaacaaattg gctatacgct atttacccgc aaagataata atatcagcct caataaagcc
    15601 ggtcaggaac tgtatcaaaa actgttcccg gtttatcaac gactttctgc gattgataat
    15661 gaaatccata actcagggcg tcgttcacga gaaattgtga ttggcataga taatacttat
    15721 ccgacaatta ttttcgatca gttgattagt ctcggagata agtatgaagg cgtgaccgcc
    15781 cagccagttg agttcagcga aaacggtgtt atcgataatc tttttgaccg tcagttagat
    15841 tttattatct ccccacagca tgtatctgca cgagttcagg aactggaaaa cctgactatc
    15901 agtgaattac caccgctgcg attgggcttt ctcgtttctc gccgttatga agagcgtcaa
    15961 gagcaagagc tgcttcagga gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac
    16021 ttcgaagcaa tgattgatgc gaatatgcga ccttgtggga tcaatcccac cattatttat
    16081 cgtccctaca gttttatggc caaaatcagc gccgtggagc gcggacattt tctgacggtg
    16141 atcccgcatt ttgcctggcg actggtgaat ccggcaacgc tgaaatattt tgatgcgccg
    16201 cacaggccga tgtatatgca ggaatatctt tattcaatca gaaatcatcg ttataccgcc
    16261 acgatgcttc agcatattgc tgaagatcgt gacgggacaa accattaacc cagcatcgag
    16321 ccggttgcaa ttaaattacg gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt
    16381 ccgccgggcg cattttccag atagcgatgc agataatccc gatacatagg atgtgcacaa
    16441 ttatcaatga tagtgcgggc gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct
    16501 tgttcggtga tgatcacttt gacgctgtgt tcgctgtgat caacatggct gcacattggc
    16561 acgacggttg agatcttgcc ttctttagca atcgacggag ccataaagat cgacagatac
    16621 gcgttgcgtt caaaatcacc gctgccgccg atgccgttca tcagatcgac cccggctacg
    16681 tgcgttgagt tggcatgccc gtaaatatca aactccaggc cgacgttcag agcgatgacg
    16741 cccagacgac ggatgatttc cgggttattg gaaatctcct gcggacgcaa cacaatgcgg
    16801 ctggcaaagt aatccatatt gtcgtaaatc ttgcgcaggg aatcggccga gattgtcagg
    16861 ctggaggcgc tggccccgct gattttgccg gtttccagta aatgcaccac cgattcctgt
    16921 agcacttccg aatacatcat gaacggagga atttccgggt tttcccccag acgcgccatt
    16981 accgcattat tgatattgcc cacgccactt tgcagcggca gaaattccgg cggaatacgc
    17041 ccatgcgcca tttcctgcaa taagaacgtg accacgttat cggcaatctg ctggcacatg
    17101 ggattttgct tatccagcat attaccggcg tcgggcaagt tggtttccac gacggcgaca
    17161 atctttttcg gatcgatttg cacatagcgg gtaccgacgc gatccattgc atggaagatc
    17221 gacacgctat tgcgccgcgg tggcgcgcca ggaatcacaa tatctgccag ttctgcaacg
    17281 cgcggatcgt gatagtgatt aagttcaatg atcactttct tcgctcgcag cagccaggtc
    17341 ggcgcattac cgatcccgct ggttaaccag actcgaccat ccggtgccag tgccgatgct
    17401 tcaatgacgg caacatcaat gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg
    17461 ctcaaatgca ggtcaacgaa actcaccgcg ccctgattga tctttttacg taaaccggac
    17521 gatgtttgat atgacgcacg ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg
    17581 gcggcgctga ttgacgcccc cgtcagcagg cgaatttgat acggcttttt ggcctcatgc
    17641 tgttcgttag ctctgcgggc aatcgcggtg ggtagggctt tcggcgaacc cgccggggta
    17701 aagccgctaa atgccaccat gtcgttatgc tggataattt ctgccgcttc atcggcggtc
    17761 atccttgtcc actgagtttc catttttact ccttcgttcg cagggattaa tgaccaacga
    17821 aattaggttt acgtttttcg aggaaagcgt tcattccttc ctggtaatct tcgctgtcat
    17881 aaaccgcgcg tcgcatcccc tgaatacgtt caaattcatc ggagttcatg gtgtgcgctt
    17941 cgcccagtac acgcagctct tctttgataa cagcaatggc taacggcgct ttctcagaga
    18001 tgtggtgcgc catttgtaag gtgaaatctt ccagttcttc cacttccaca acatggttga
    18061 ggatgccgac agccaacgcg cgctgggcgg tgattggcga agcggtaaaa atcagttctt
    18121 tgacaatgtg gaagcccgcg tcgcgggtca ggttgtgaat gccgaccagg ttatacggga
    18181 cgccgaggtt tacaggcgtc attgagaagg ttgaggtact ggcggcgata atcagatcgg
    18241 aactcatgat catttcaaat gcgccacccc aaacactacc ttccaccatc gaaatgatcg
    18301 gtttcgggaa tttttggatc atgcgggtga tttgacgcaa tggatcatca taggagagcg
    18361 gatcgcgacc gccagacggc agttcgtgaa tatcgtggcc tgcggagaag actttggatc
    18421 cactcggcgc gcgcaaaatg atacagcgaa tttccggccg gttgagatcg cttaatgcct
    18481 gcataagatc atcaataaag actttactta aggcattaag tttgcggcca tagttaaact
    18541 caatgaccgc cactttgttg atagtgacaa cgttaacata ctgataagac ataaaaattc
    18601 ctttaatcaa aatattgcgt ctggataaat tcactgagct gccgcaggcc ggtgcgcggt
    18661 gagagcgtat tgtttttgac cgctaaaagc gtctggcggt aatagcgatc gaaatcttca
    18721 ttcgcgaaca ggtgattcag tacttcttct tcggtctgct tacgcagcca ttccaccgat
    18781 tgttgttgcc gcacttgttg taaacgacca ctggcagtta gcgcggtttt gaagtcgatg
    18841 atggcgtgcc agatctcatc gattccacgt ttttccagtg cgctacaagt cagaacccgt
    18901 ggctgccatt cgtcgtattt acgtcgcaga atatgcaggg cactctcgta catatgccgg
    18961 gcaatggcga cattggtatg gttatcgcca tcgtctttgt tgataacgat cagatcagcc
    19021 acttccatca gccctttttt aatgccctgc agatcatcgc cgccaccggc aatttgcaac
    19081 gagataaaac agtccaccat gcgggcgact tctgtttccg actgcccgac gccaaccgtt
    19141 tcgacaatca ctacgtcata acccgctgct tcgcataaca gcattaattc ccgcgctcgc
    19201 tgactggcac cgcccagatg accggaggat ggtaccgggc gaataaacgc cgcttcggca
    19261 cgcgccaggt cattcatgcg ggttttatcc ccgagaatgc taccgccagt gaccgggctg
    19321 ctgggatcga ccgcaataac cgcgaccttt aatccctctc gaatcaacaa catgccaaag
    19381 gcctcaagaa aggtactttt ccccgcgccg ggggtgccgg taacgcccag tcgcagggtg
    19441 ttaccgcagt acggcataat ggcatcaagc agctgcgtac ttagtgcctg atgacgcggg
    19501 tgacggcttt ccaccagcgt catggcctgg gcgagtgtgg cacgctcacc ctgacgtaag
    19561 cggcgaatac tttctgccag cgtggcttca ttaatcatga tgctggctta tcagattcag
    19621 tacgtcgcgc acactgtcga gcataggtgt acctggacca taaatcgccg ccacgccgcg
    19681 ctcttgcagg aaggcgtaat cctgcggcgg aatgacgcca cccgcgacca cgcagatatc
    19741 ttcgcgtccc cattttttca gcgcttcgac cagttccggg atcagcgttt tatgaccggc
    19801 agccagtgag gatgcgccca ctacgtgaac gtcgttttct acggctaggc gggcgatctc
    19861 ttcaggtgta gagaacatcg ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct
    19921 ggcgatcact ttcgcgccgc gatcgtgtcc atcctggccc atcttagcga tcagaatgcg
    19981 cgggcgacga ccattgtcgg caaggaactg ctccgtttgc gcaacaatgg catcgaactc
    20041 ggaggccgat ttctcagact gatgatagct ttgcgcaatc acgccggtaa cacactggct
    20101 tggcaccaga taacggtcga aagcgacttc cagcgcatcg gaaatttcac ccagggtggc
    20161 gcgaacgcga gcggcattaa cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg
    20221 agtcagggcg ttcaacgcgg cggttacggc ggcatcatca cgggtggcgc gaatgcgttc
    20281 cagcgaagca atttgctcgt tacgcaccat cacgttgtcg atctcaagta catcggtttc
    20341 gtcttcgtga tccagtttgt acttgttgac accaacgatg acacgcttgc cctggtcgat
    20401 cagcgactgt tcgcgcgctg aggcctcttc gatcattcgt tttggcagac ctgcttcgat
    20461 cgctttcgcc atgccaccgg cttcgtcgat ctgttggata atagctctgg cttgtttgac
    20521 gatttgatcg gtcagcgact caatgtaata ggatccggcc agtggatcga cggtgcggca
    20581 gagttctgat tcttcctgga tgatgatctg ggtgttgcgg gcaatgcgtg ctgagaaatc
    20641 ggtaggcaaa ccaagcgctt cgtcaaaggc gttggtatgc agtgactgag taccgcccag
    20701 cgtcgcagcc agtgcttcaa tggtggtgcg gataacgttg ttatacggat cctgttcagt
    20761 caggctccag cctgaggtct ggcagtgggt acgcagcgcc agtgatttcg gatcctgtgc
    20821 gccaaatcca ctgaccgctt cgctccataa ataacgtgcc gcacgcaaca tggcgacgtt
    20881 cataaacaga tccatgccga taccgaagaa gaacgacagg cgaggagcga agtcatcaat
    20941 tttcagtccg gcagagattg ctgctttgat gtactcaatc ccatcagcga gcgtaaatgc
    21001 tacctgctgc acgcagttgg cacccgcttc ccccatgtgg taaccgctga tactgatggt
    21061 attaaatcgc ggcatgttgc cggaacacca ggcgatgatg tcggcgataa tgcgcattga
    21121 cggttttggt gggtaaatat aggtgttgcg gcagaggtac tctttgagga tatcgttctg
    21181 aatggtgccg gtcagtttat caggtgtaac accttgctct tctgcggcga cgatataaaa
    21241 cgccagtact ggtagcactg cgccattcat ggtcatcgaa accgacattt tatccagcgg
    21301 gatctggtcg aacaggactt tcatatcttc cacggtgtcg atagcgacgc ccgctttgcc
    21361 gacgtcgccc gccacgcgcg ggttatcgga gtcgtagcca cggtgggtgg caaggtcaaa
    21421 cgcaacggaa agaccttttt gcccggcggc caggttacgg cgataaaaag cgttggactc
    21481 ttttgctgtt gaaaaaccag catactgacg gatggtccac ggttgggcgg tatacatagt
    21541 ggcacgcggg ccacgaacgt agggcggcaa accaggaagg gtacctgtca cctccagatt
    21601 atcgagatcg gcttcggtat acagcggctt gatggcgatc ccttccgcgg tttgctgaac
    21661 cagcgagtcg acagttttct cccgacggct caattccttg ttggcaagct gttgccactc
    21721 ctgcacgtta gacattattt gctccgtaaa acgcaatcaa aatgagggta tttgcgccta
    21781 cggtgagctt tttacgtggg tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt
    21841 tgccgcctga gatttgtcta ttttgtgatg tgattcaagg aatacaaaaa gaagtgagga
    21901 caacgcctga tatgtgctat caggcattat ttgatggatt aatcctgacg aaggactttg
    21961 tgaccataat cgagtaacgc atcagtgact ttacgcatca tgcggctttc aggagcaaag
    22021 cggtgccagt agagcatccg tcgttgaaat agcccaggcg ttaagtcaat cagttcaccg
    22081 ctggccagct ctttctcgat ttgcaggtgc gggatcatac agcaggtggt gccctggcga
    22141 gcaagttgta cgaacgcttc tgaagaatta acgatatggc agggcacgct gcctggaggc
    22201 agatcgaagt tttgctgcaa aaaggcctgg tgcatatcgt caagatggtc aaacgcgacc
    22261 actggcgctt tcagtaatgc cgaacgcgtt acgccgttag ggaaatattt ttcggcaaag
    22321 ggttttgagc tgacgaacag atagtcgagc gcaccaagtt tatcgacaag acaactcggc
    22381 agcgcctgat gttgaatact caccgcgccg accacttcgc cgcggcgcag acgttcctga
    22441 gtgcgggttt catcttctac ctgcaagttg aggcggatag gcgaatcagc caacacagga
    22501 gccagtgcag gaagcaacca cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc
    22561 agcggagtcg aaccggtttg ttcatcgccc agccactctt cttccagcaa ctccacctgg
    22621 cgcagcagtg ccagcagttt ttgcccttgt tccgtcgggc gcggcggtac ggtacgcacc
    22681 aacagcggct gcccgaacat attttccagt tgcttaatgc gctgtgagac ggctgattgt
    22741 gtaatgcaca gcttttgtgc cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt
    22801 gcctgtaatg ttctgtagtc cgggcgtttc attgctgttg tctgctcctg gtaaatgctt
    22861 tttcctgcac tatgacacaa ttttgtgtca ggttgcatat accgaagccg tcggcgggta
    22921 acatttgtat tcgtggttaa acgcgcagct taaaagatca gggatttgcg ctaatgcatt
    22981 tcgctcagcg agttcgcgcc ctggttgtgt tgaatggcgt ggcgttattg cctctgttcg
    23041 caccgtggag cggcataccc agaccgttgt atgctttatt tgcggggcga aaggggatgc
    23101 ccgccattgc gcgatatttt atggatgagt taaccacgcg gcttgccaac ggggtctgaa
    23161 tcgctttttt tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg
    23221 caggatgaat tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggcacc
    23281 attgttggtg taggtacagg ttccaccgcc gcacacttta ttgacgcgct cggtacaatg
    23341 aaaggccaga ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc
    23401 ctcggcattc acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc
    23461 gcagatgaaa tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt
    23521 gaaaaaatca ttgcttcggt tgcagaaaaa tttatctgta ttgcagacgc ttccaagcag
    23581 gttgatattc tgggtaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca
    23641 gtggcgcgtc agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtggtgacc
    23701 gataatggca acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg
    23761 gaaaacgcca taaatgcgat tcctggcgtg gtgactgttg gcttgtttgc taaccgtggc
    23821 gcggacgttg cgctgattgg cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac
    23881 gggggaacct cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgct
    23941 tttaccaggc aattgtcgat tgctctaaat aaatcctcta aaccagcata ttcatccaag
    24001 aattaccttt gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata
    24061 ttattttttg atatgttgaa aggcggatgc aaatccgcac acaacatttc aaaagacagg
    24121 attgggtaaa tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa
    24181 ggcgtgcacc aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt
    24241 cacaaaggcg cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc
    24301 ggcctgcgat cccgtaccca tctgactgaa gacgtgatta acgccgcaga aaaactggtc
    24361 gctattggct gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc
    24421 gggatcccgg tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt
    24481 ggcgaactgc tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcaccgtggc
    24541 gtgtggaaca aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc
    24601 ggctacggtc atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt
    24661 tacttttatg atattgaaaa caaactgccg ctgggcaacg ccactcaggt acagcatctt
    24721 tctgacctgc tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtccacc
    24781 aaaaatatga tgggcgcgaa agagatttcg ctaatgaagc ccggctcgct gctgattaat
    24841 gcctcgcgcg gtactgtggt ggatattccg gcgctgtgtg atgcgctggc gagcaaacat
    24901 ctggcggggg cggcaatcga cgtattcccg acggaaccgg cgaccaatag cgatccattt
    24961 acctctccac tgtgtgaatt cgacaacgtc cttctgacgc cacacattgg cggttcgact
    25021 caggaagcgc aggagaatat cggcctggaa gttgcgggta aattgatcaa gtattctgac
    25081 aatggctcaa cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcatggtggt
    25141 cgtcgtctga tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct gaacaaaatc
    25201 ttcgccgagc agggcgtcaa catcgccgcg caatatctac aaacttccgc ccagatgggt
    25261 tatgtagtta ttgatattga agctgacgaa gacgttgccg aaaaagcgct gcaggcaatg
    25321 aaagctattc cgggtaccat tcgcgcccgt ctgctgtact aattcccctt ctctgaaaat
    25381 taacgggcag gtcactgact tgcccgtttt tttatccctt ctccacaccg ttgccgcgtt
    25441 gcgcattcta ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt
    25501 gtttatttaa tggggattta cgatggcggg acagcatggg gctgtaacgg taaagccctc
    25561 accgaagcga gggcttaaag gagaagggtt atgatgcgac ttgtcatcat actgattgta
    25621 ctgttactga taagtttcgg agcttattaa cagtcagtct caggggagga gtcatcctcc
    25681 cttaccctta ctcactaaat taggtcaaag aatcaacgat gtcaatcagg gcgatgcggg
    25741 gtgcatcgcc ctgaaccgaa ctttttgata gccacttcat cgcccacgtt tactgccgta
    25801 atagatcctt tttcccaaaa attatctctg ttttgcatgg ggtttttcga gcacgctcga
    25861 agaaaagatt taaaggtaga tcaatgcgtt gtggttattt aaagagagct tacgatagct
    25921 gggcagcatg gggctgtaac ggtaaagccc tcaccgaagc gagggcttga aggagaaggg
    25981 ttatgatgcg acttgccatc atactgattg tactgttact cataagtttc ccagcttatt
    26041 aacagtcagt cacaggggag gagcaatcct cccttaccct tactcactaa attaggttaa
    26101 agaatcaacg atgtcaatca gggcgatgca ggtgtatcgc ccttaccact cccagacttt
    26161 cgacggtgta accaccgcag gaagagggat atcccactct tcaacgggga gtttttccac
    26221 caactgacaa tcatgcgcat aacccaccgg ttgcgtttta tagtgctgcc agttttgtaa
    26281 ggtccgatca taaaaaccac cgcccattcc caggcgctga ccgtactcat caaaggcgac
    26341 cagcggtgtg atcagcacgt ctaatcggga aaggggtagc acgtcacgca catccagttt
    26401 tggctcatgg atcttcaacc tgttcatcac cagttcgctt tgcggatggt aattcaggaa
    26461 cagcaaatta ccggcactaa agggatgcaa aactggaaga tatacgcgct taccggcgcg
    26521 ccagagttgt tctattagcg gctgggtgtc gagttcacca tcaaaagaga ggaatacagc
    26581 gaccgtatgc gccatcacca ccgggggata agtcatcatc cgggtagcgg cttgttgacc
    26641 catttcctgc tgttgttccg gcgttaacgc acgacgacgt tgccgaatca ttttgcggat
    26701 ttcttgtcgg gataatgtca gtgggagttc tggtagttgc gtcatggctg gtgccagata
    26761 agaagggaat ctccgagatg ccgccgcagg ctgtaaccct tgaacccttg gttcaaggtg
    26821 aatgtgtcgt cgcagtttta aggcttctcg gacggaccga gcatgctcac caaccgcgga
    26881 gcgccacatt cttgtggtat gaaatatcgg ctcaggggac tggcccgctt gcgaacatct
    26941 cagagaaatt ttgtcttcac ggttactcta ccacagtaaa ccgaaaagtg tcattcaaag
    27001 ttttggttag ttttttcggt gatgcgacct tgttcaagta acgcttgttc tatggtctgc
    27061 tgcagcatcc gaatacgctg ttccatactt gccgcgtagt cacgagtctt cgctttttct
    27121 tgcgctaact catagctgat attcaatgcg gcaatgaaga ccaactgttc agtatttgtg
    27181 actctagtgc gttctttcag atcttgcaac cgttggttca gatcgtccgc tgcctgattc
    27241 aacgcatccc tttggtcagg cgggcagttc acacgcagtg aacggccaaa aatttggata
    27301 tcgacgggtt gtgcagacat gccaccttcc tgctgattga ctgcgctgcc ttcgtcttta
    27361 gacccatggt ctgcgaaggg gcgacactat agctaccctg atgagaagag acaagccctt
    27421 ttctggtcca ccagggccca aagtggtagc atatcatgaa tattcctccc tttgacgacg
    27481 aatgcttatg tctatacaga acgaaatgcc tggttacaac gaaatgaacc agtatctgaa
    27541 ccaacaaggg acgggtctga cccctgctga gatgcatggt ttaatcagtg ggatgatatg
    27601 tggcggtaac gatgacagct catggctacc gctacttcac gacctgacga acgaaggcat
    27661 ggctttcggt catgagctgg cacaggcact gcgtaaaatg cactctgcca ccagcgatgc
    27721 cctgcaggat gacggcttcc tttttcagct ttatctgcct gatggcgatg atgtcagcgt
    27781 tttcgatcgg gctgatgcat tggcaggttg ggtcaatcac ttcctgcttg gtcttggcgt
    27841 tacgcaaccg aagctggata aagtgaccgg cgaaaccggt gaagctatcg acgatctgcg
    27901 taacattgcg caactgggtt acgacgaaga cgaagatcag gaagagcttg aaatgtcgct
    27961 tgaagagatc atcgaatacg ttcgtgttgc cgcgctgtta tgccacgaca cctttactca
    28021 tccgcaaccg accgcgccag aagtacaaaa accgactcta cactaaaaac aaaaaacgta
    28081 aggagagtgt tatgagtgag atatcccggc aagagtttca gcgtcgccgt caggccctgg
    28141 tggagcaaat gcaacccggc agcgccgcgc tgatttttgc tgcaccagaa gtaacacgta
    28201 gcgccgacag cgaatacccc tatcgtcaga acagtgactt ctggtacttc accggcttta
    28261 acgaaccgga agcggtgctg gtgctgatta aaagcgatga cactcataac cacagcgttc
    28321 tgtttaaccg cgttcgcgac ctgacggcgg agatctggtt tggccgtcgc ttaggccagg
    28381 atgccgcgcc agagaaactg ggcgttgacc gcgcactggc attcagcgaa atcaatcagc
    28441 aactttatca actacttaac ggcctggatg tggtttacca tgcccagggc gaatatgcat
    28501 atgctgatga aatcgtgaac agtgcgctgg aaaaactgcg taaaggttcg cggcaaaatc
    28561 tcaccgcacc ggcaacgatg attgactggc gtcctgttgt tcatgaaatg cgcctgttca
    28621 aatcgccaga agagattgcc gtactccgcc gcgcgggaga aatcaccgcc atggcacata
    28681 cccgggcgat ggaaaaatgc cgtccgggaa tgttcgagta ccatctggaa ggcgaaattc
    28741 accacgaatt taaccgccac ggtgcgcgct atccgtccta taacaccatt gtcggcagcg
    28801 gtgaaaacgg ctgcattctg cactacaccg aaaacgagtg tgaaatgcgc gacggcgacc
    28861 tggtgttgat tgacgcgggt tgcgaataca aaggttacgc tggcgatatt acccgcacct
    28921 tcccggtcaa cggcaaattc actcaggccc agcgtgaaat ctacgacatt gtgctggagt
    28981 ctctcgaaac cagcttgcgc ctgtatcgtc cgggaacttc cattctggaa gtcactggtg
    29041 aagtggtgcg catcatggtt agcggcctgg taaaactcgg cattctgaaa ggtgatgttg
    29101 atgaactgat cgctcagaac gcccatcgtc ctttctttat gcatggcctt agccactggt
    29161 tagggctaga tgtccatgac gttggcgttt atggtcagga tcgctcgcgc attctggaac
    29221 cggggatggt actgaccgta gagcctggtc tgtatatcgc gccggatgcg gatgtgccag
    29281 aacaatatcg cggtatcggc attcgtattg aagacgacat tgtgattacc gaaaccggca
    29341 acgaaaacct caccgccagc gtggtgaaaa agccggaaga aatcgaagcg ttgatggcag
    29401 ctgcgagaaa gcaatgagcg taatcatcgt cggtggcggc atggcgggcg cgacgctggc
    29461 gctggctatt tcccggttaa gtcacggggc gctgccggta catttgattg aagcgaccgc
    29521 gccagagtca catgctcatc cgggctttga tggacgagct attgcgctgg ctgcggggac
    29581 ctgtcagcaa ctggcgcgca tcggcgtctg gcaatctctg gcggattacg caaccgccat
    29641 cactaccgtg catgtcagcg atcgtggtca cgctggattt gtcaccctcg ccgcagaaga
    29701 ttaccaactg gcggcgctgg gacaggttgt cgaattgcac aatgtcgggc aacggctgtt
    29761 tgcattgctg cgtaaagcac ctggcgtaac gctgcattgc cctgatcgcg tggctaacgt
    29821 tgcccgtact cagagtcacg ttgaagtgac gctggagagt ggcgagacgc tgacgggccg
    29881 cgtgctggta gcagctgatg gcacccattc agcgttagcc accgcgtgcg gcgttgactg
    29941 gcagcaggag ccttacgaac aactggccgt gattgccaac gttgcgactt ccgttgcgca
    30001 cgaaggccgc gcttttgaac gcttcacgca acatggcccg ctggcgatgt tgccgatgtc
    30061 tgacggacgc tgttcgctgg tctggtgtca tccactggaa cggcgcgaag aggtgctgtc
    30121 gtggagcgac gagaagtttt gccgtgaact ccagtcggcc tttggctggc gacttggaaa
    30181 aattacccac gccggtaaac gcagtgctta tccgctggcg ttaacccgcg ccgccagagc
    30241 tattacccat cgtaccgtgc tggtgggcaa tgcggcgcaa actctgcacc cgattgccgg
    30301 gcaagggttt aacctcggta tgcgagatgt catgagcctt gcggaaaccc tgactcaggc
    30361 gcatgagcgc ggagaagaca tcggtgatta cggcatattg tgccgctatc agcagcgtcg
    30421 acagagtgat cgcgaagcaa ccatcggcgt cacggacagc ctggtacatc tttttgccaa
    30481 ccgttgggca ccgctggttg tcgggcgcaa catcggactg atgacgatgg aattattcac
    30541 cccggcacgc gatgtgctgg cgcagcgcac cctcggttgg gtggcgcgtt gaaggcggtt
    30601 ttaaggaata aacaatgcaa agtgttgatg tagccattgt tggtggcggc atggtggggc
    30661 tggcggttgc ctgcggctta caggggagcg gcttacgcgt tgccgtactg gagcagcgcg
    30721 taccggaacc tctggcggcg gatgcaccac cacaactgcg cgtttcggct atcaatgccg
    30781 ccagcgaaaa attactcacc cgtcttggcg tctggcagga cattctctct cgtagggcca
    30841 gttgttatca cggtatggaa gtgtgggaca aagacagttt tggtcacatt tcgtttgacg
    30901 atcaaagcat gggctatagc catcttgggc atatcgttga aaactcagtg attcactacg
    30961 cgctgtggaa caaagcgcag cagtcgtcag atatcactct gttggccccc gcagaattac
    31021 agcaggtcgc ctggggagaa aatgaaactt tcctgacgct gaaagatggc agcatgttaa
    31081 cggcgcgtct ggtgattggc gcggacggtg ctaattcctg gttgcgcaac aaagcagata
    31141 ttccgctgac tttctgggat tatcagcatc acgcgctggt agcgaccatt cgcacggaag
    31201 aaccgcatga tgcggtggcg cggcaggttt tccatggcga aggcattctg gcctttttac
    31261 cgcttagcga tccgcatctt tgctcaattg tctggtcact gtcgccagag gaagcgcagc
    31321 ggatgcagca ggcaagtgaa gatgaattta atcgcgcgtt aaatatcgct tttgataatc
    31381 gcctgggctt atgcaaggtt gagagcgcgc gtcaggtgtt cccactgacg gggcgttatg
    31441 cgcgccagtt tgccgcgcac cgtctggtgc tggtgggcga cgccgcgcat accattcacc
    31501 cgctggcggg gcagggagta aatctcggct ttatggatgc tgcagagctg attgctgaac
    31561 tgaaacggtt gcatcgtcag gggaaagaca tcgggcagta catttacctg cgccggtatg
    31621 agcgtagccg caagcacagt gcggcgctga tgctggctgg tatgcaggga ttccgcgatc
    31681 tgttttccgg tactaatccg gcgaaaaaac tgctgcgtga tattggtttg aaactggccg
    31741 acacgcttcc tggcgttaaa ccacaactta tccgccaggc aatgggatta aacgatttgc
    31801 ctgaatggct gcgttaaaaa tttctcttct gctgtttatt tgatacccat cacactttca
    31861 tctcccggtt ttttcgccgg gagattttcc tcatttgaaa taaactaatt tcacctccgt
    31921 tttcgcatta tattttctaa tgtcacgaat ttttgattta gtgttttttg acattttttc
    31981 agctcttaat attgccttat tcaaattaac tttctcatca catcatcttt atataaaaac
    32041 ttgtgtattt tttggttttt tattctgttg cgattttggc gttttttaac cataagctaa
    32101 tgtgatgatc aattttacct tatggttaac agtctgtttc ggtggtaagt tcaggcaaaa
    32161 gagaacgatt gcgttgggga ccgggagtgg ctccgatgct gggtttcgtg gtgataattt
    32221 caccatgaaa aagttgtcag ccccgcttat tcaatgagga caagatggca caacagactc
    32281 ctttgtacga acaacacacg ctttgcggcg ctcgcatggt ggatttccac ggctggatga
    32341 tgccgctgca ttacggttcg caaatcgacg aacatcatgc ggtacgtacc gatgccggaa
    32401 tgtttgatgt gtcacatatg accatcgtcg atcttcgcgg cagccgcacc cgggagtttc
    32461 tgcgttatct gctggcgaac gatgtggcga agctcaccaa aagcggcaaa gccctttact
    32521 cggggatgtt gaatgcctct ggcggtgtga tagatgacct catcgtctac tactttactg
    32581 aagatttctt ccgcctcgtt gttaactccg ccacccgcga aaaagacctc tcctggatta
    32641 cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg tgatgacctt tccatgatcg
    32701 ccgtacaagg gccgaatgcg caggcaaaag ctgccacact gtttaatgat gctcagcgtc
    32761 aggcggtgga agggatgaaa ccgttctttg gcgtgcaggc gggcgatctg tttattgcca
    32821 ccactggtta taccggtgaa gcgggctatg aaattgcgct gcccaatgaa aaagcggccg
    32881 atttctggcg tgcgctggtg gaagcgggcg ttaagccatg cggtctgggc gcgcgtgaca
    32941 cgctgcgtct ggaagcgggc atgaatcttt atggtcagga gatggacgaa actatttctc
    33001 ctttagccgc caacatgggc tggactatcg cctgggaacc ggcagatcgt gacttcatcg
    33061 gtcgtgaagc cctggaagtg cagcgtgagc atggcacaga aaaactggtt ggtctggtga
    33121 tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg ttttaccgat gcgcagggca
    33181 accagcatga aggcattatc accagcggga ctttctcccc gacgctgggt tacagcattg
    33241 cgctggcacg cgtgccggaa ggtattggcg aaacggcgat tgtgcaaatt cgcaaccgtg
    33301 aaatgccggt taaagtgacg aaacctgttt ttgtgcgtaa cggtaaagcc gtcgcgtgat
    33361 ttactttttt ggagattgat tgatgagcaa cgtaccagca gaactgaaat acagcaaaga
    33421 acacgaatgg ctgcgtaaag aagccgacgg cacttacacc gttggtataa ccgaacatgc
    33481 ccaggagctg ttaggcgata tggtgtttgt tgacctgccg gaagtgggcg caacggttag
    33541 cgcgggcgat gactgcgcgg ttgccgaatc agtaaaagcg gcgtcagaca tttatgcgcc
    33601 agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc gattccccgg aactggtgaa
    33661 cagcgaaccg tatgcaggtg gctggatctt taaaatcaaa gccagcgatg aaagcgaact
    33721 ggaatcactg ctggatgcga ccgcatacga agcattgtta gaagacgagt aacggcttta
    33781 ttcctcttct gcgggagagg atcagggtga ggaaaattta tgcctcaccc tcactctctt
    33841 cgtaaggaga gaggttcaca attcactgca cgtttcagga accatcgctc atgacacaga
    33901 cgttaagcca gcttgaaaac agcggcgctt ttattgaacg ccatatcgga ccggacgccg
    33961 cgcaacagca agaaatgctg aatgccgttg gtgcacaatc gttaaacgcg ctgaccggcc
    34021 agattgtgcc gaaagatatt cagcttgcga ccccgccgca ggttggcgca ccggcgaccg
    34081 aatacgccgc actggcagaa ctcaaggcta ttgccagtcg caataaacgc ttcacgtctt
    34141 acatcggcat gggttacacc gccgtgcagc taccgccggt tatcctgcgt aacatgctgg
    34201 aaaatccggg ctggtatacc gcgtacactc cgtatcaacc tgaagtctcc cagggccgcc
    34261 ttgaagcact gctcaacttc cagcaggtaa cgctggattt gactggactg gatatggcct
    34321 ctgcttctct tctggacgag gccaccgctg ccgccgaagc aatggcgatg gcgaaacgcg
    34381 tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc cgatgtgcat ccgcaaacgc
    34441 tggatgtggt ccgtactcgt gccgaaacct ttggttttga agtgattgtc gatgacgcgc
    34501 aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt acagcaggtt ggcacaaccg
    34561 gtgaaatcca cgactacacc gcgcttatta gcgaactgaa atcacgcaaa attgtggtca
    34621 gtgtcgccgc cgatattatg gcgctggtgc tgttaactgc gccgggtaaa cagggcgcgg
    34681 atattgtttt tggttcggcg caacgcttcg gcgtgccgat gggctacggt ggcccacacg
    34741 cggccttctt tgcggcgaaa gatgaataca aacgctcaat gccgggccgt attatcggtg
    34801 tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc gatgcagact cgcgagcaac
    34861 atattcgccg tgagaaagcg aactccaaca tttgtacttc ccaggtgctg ctggcaaaca
    34921 tcgccagcct gtatgccgtt tatcacggcc cgattggcct gaaacgtatc gctaaccgca
    34981 ttcaccgtct gaccgatatc ctggcggcgg gcctgcaaca aaaaggtctg aaactgcgcc
    35041 atgcgcacta tttcgacacc ttgtgtgtgg aagtggccga caaagcgggc gtactgacgc
    35101 gtgccgaagc ggctgaaatc aacctgcgta gcgatattct gaacgcggtt gggatcaccc
    35161 ttgatgaaac aaccacgcgt gaaaacgtaa tgcagctttt cagcgtacta ctgggcgata
    35221 accacggcct ggacatcgac acgctggaca aagacgtggc tcacgacagc cgctctatcc
    35281 agcctgcgat gctgcgcgac gacgaaatcc tcacccatcc ggtgtttaat cgctaccaca
    35341 gcgaaaccga aatgatgcgc tatatgcact cgctggagcg taaagatctg gcgctgaatc
    35401 aggcgatgat cccgctgggt tcctgcacca tgaaactgaa cgccgccgcc gagatgatcc
    35461 caatcacctg gccggaattt gccgaactgc acccgttttg tccgccggaa caggctgaag
    35521 gttatcagca gatgattgcg cagctggctg actggctggt gaaactgacc ggttacgacg
    35581 ccgtttgtat gcagccgaac tccggcgcac agggcgaata cgcgggcctg ctggcgattc
    35641 gtcattatca tgaaagccgc aacgaagggc atcgcgatat ctgcctgatc ccggcttctg
    35701 cgcacggaac taaccccgct tctgcacata tggcaggaat gcaggtggtg gttgtggcgt
    35761 gtgataaaaa cggcaacatc gatctgactg atctgcgcgc gaaagcggaa caggcgggcg
    35821 ataacctctc ctgtatcatg gtgacttatc cttctaccca cggcgtgtat gaagaaacga
    35881 tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca ggtttacctt gatggcgcga
    35941 acatgaacgc ccaggttggc atcacctcgc cgggctttat tggcgcggac gtttcgcacc
    36001 tcaacctgca taaaactttc tgcattccgc acggcggtgg tggtccgggt atgggaccga
    36061 tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca tagcgtggtg caaatcgaag
    36121 gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt cggtagcgcc tctatcctgc
    36181 caatcagctg gatgtacatc cgcatgatgg gcgcagaagg gctgaaaaaa gcaagccagg
    36241 tggcaatcct caacgccaac tatattgcca gccgcctgca ggatgccttc ccggtgctgt
    36301 ataccggtcg cgacggtcgc gtggcgcacg aatgtattct cgatattcgc ccgctgaaag
    36361 aagaaaccgg catcagcgag ctggatattg ccaagcgcct gatcgactac ggtttccacg
    36421 cgccgaccat gtcgttcccg gtggcgggta cgctgatggt tgaaccgact gaatctgaaa
    36481 gcaaagtgga actggatcgc tttatcgacg cgatgctggc tatccgcgca gaaatcgacc
    36541 aggttaaagc cggtgtctgg ccgctggaag ataacccgct ggtgaacgcg ccgcacattc
    36601 agaacgaact ggtcgccgag tgggcgcatc cgtacagccg tgaggttgca gtattcccgg
    36661 caggtgtggc agacaaatac tggccgacgg tgaaacgtct ggatgatgtt tacggcgacc
    36721 gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca gtaattcact gattcgacta
    36781 ttttctaaag gcgcttcggc gcctttttag tcagatgaca aagtacaaaa gtgctcagac
    36841 agtcccctcg cccctttggg gagagggtga ggggaacagg ccggcactgg cgcgaatatt
    36901 taccctcacc ccggccctct ccctgaaagg gcgaggggga aaagcgtgcc aacattgaag
    36961 attgagccag tttgttagca atctcaaaga tacgtcaacg aatgaatttt tctcggaaaa
    37021 acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg caactgcatt
    37081 actgttggcg caagaagggt atacggtggc ggttaattat cagcaaaacc tccacgcggc
    37141 gcaggaagtg atgaacttaa taacgcaagc cggtggcaaa gcattcgtgc tccaggcgga
    37201 tatcagcgac gaaaaccagg tcgttgcgat gtttacagca atcgatcagc acgatgaacc
    37261 gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg ttgaaaacct
    37321 taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt ttctctgctg
    37381 ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg ctatcgtcaa
    37441 tgtctcttcg gtggcctcac ggttgggttc gccaggggaa tatgttgatt acgcggcatc
    37501 gaaaggggcg attgatacgt taaccaccgg actatcgctg gaagtcgccg cgcaggggat
    37561 ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca gcggcggcga
    37621 gcctggacgc gtcgatcgcg ttaagtcgaa catccccatg cagcgtggtg gacaggcaga
    37681 agaggtcgcg caggccattg tctggctact aagtgataaa gcctcttacg tcacgggaag
    37741 ttttatcgat ttggcgggcg ggaaataaaa cagggaagtt gtctgaccgg atgcaacaag
    37801 tattgcatcc ggtacttcat cgacttaaag cttctcgccg ttgctggcaa tcacctcttg
    37861 gtaccagtta aagctcttct tacgtgaacg cgacatatcg ccagcaccgt cgtcatgttt
    37921 attcacatag ataaagccgt agcgtttgct gtactgcccg gtggtgaacg atacgcagtc
    37981 gatgcagccc cacggtgtgt agcccatcag atccacgcca tcgtaagtca ccgctttttt
    38041 catctcttcg atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga tgctgccatc
    38101 ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg gcctctgata
    38161 acgctcatac agttcgcaaa gggcatagcg cagacctact ggatcaatct gccagcccca
    38221 gtcagacgct ttaacatacg ggttcggtac gctgccttcg aaaccagaga tcgcatcgcc
    38281 ggtgccgcct tcggccttca ctgcgttggt catgtaatag ctgaaaccaa gataatcgca
    38341 ggtgccttca cgcagtacat cgagatcgcc gtcttccatt ttgatgttaa atccgcgacg
    38401 ctcccactcg ttcaacacat aggacgggta atagccgcgt agctgcacat cggtaaagac
    38461 gtagcgttcg cgcatcgact cctgagcgaa catcacatcg tccgggttac agcagtaggg
    38521 atagagcggc accatcgcca gcatacagcc gactttcatc tccgggttaa tgcgacgcgc
    38581 agctttcacc gccagggcgc tggcgacaaa ctggtgatgc agcacctgat acatcgtctc
    38641 ttccgggttc tcatgctcgg tataaaccac gccggagcag cagtaaccga acagcggtgc
    38701 acgccagtta cgctggttgt taatttcgtt gaaggtcatc cagtatttga ctttatgctt
    38761 atagcgttca aacaccactt ccgcgaaacg gacaaagaaa tcaaccactt tacggttggt
    38821 ccagctaccg tattgctgca ccagatgcag cggcatttca aagtgggaga gggtgatcac
    38881 cggttcgatg ttgtatttca gcagttcatc gaacatatca tcgtagaact tcagcccttc
    38941 ttcgtttggc tgagcttcat caccttttgg aaaaatacgc gtccaggcga tggaagtccg
    39001 aaaacatttg aagcccattt cggcaaatag cttgatgtct tccttgtagt gaccataaaa
    39061 atcaacggct tcatggtttg gatagtattt gcctggcaag acttctttgg tgatttcgcg
    39121 cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg ctcggccctt ttccgccttt
    39181 gttccagccg ccttcgacct gatgagcggc aactgcgccg ccccataaga aatctttcgg
    39241 taaggtgagt tttttcacta tcataggctc cagatgagtc aattcttaca ggaaatagta
    39301 ccatgcagcc aaacgacatc actttttttc aacgtttcca ggatgacatt ctggctgggc
    39361 gtaaaaccat caccatccgc gacgagtctg aatcacactt caaaacgggt gatgtgcttc
    39421 gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc gcaacctcaa
    39481 ccgtaacgct ggatacgctg actgaaaaac atgcagagca ggaaaatatg accctgactg
    39541 aactgataaa ggtcattgcc gacatctatc ccggtcagac acagttttat gtgattgaat
    39601 ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt aatttcatga
    39661 tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt cactggaact
    39721 attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctccgga gtaagttatg
    39781 gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc caacagcgtc
    39841 agtcacggca ttgggttggt gtttggtatc gttgggctgg tgttgctact ggttcaggcg
    39901 gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg cggcagtatg
    39961 atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg ggcaaaaatg
    40021 tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac ctacacgccg
    40081 tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt tatctggagc
    40141 ctggcattgc tgggtattct gtttaaactg accatcgcgc accgattcaa aattttatct
    40201 ctggtgacct atctggcgat gggctggctg tcgctggtgg taatttatga aatggcagtt
    40261 aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta ttcgctcggg
    40321 gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca cggcttcgtg
    40381 ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca ggcgtaatgg
    40441 gtatgccttt gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg gagcacaact
    40501 gccggattcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt gtgataaagg
    40561 catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa atgcctgatg
    40621 cgataaagag atttactctt cgagcgaata cggcagcggc tcgatatgca atgtattcgc
    40681 atcgtcgcgt acgcggaaga tgctatccgg ttccatatcg ttattcatga cgacctgtac
    40741 cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac gacgccagtt
    40801 ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg cgctacctgc
    40861 cagcaaccag agcgcacgtt tattggcacc acggaatttt gctcgcgcca ccatctcttg
    40921 tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat tggtcgcctg
    40981 tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaacccgctt caatgtttaa
    41041 tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat cggtcagcat
    41101 attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt caaaccatag
    41161 caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt cgctaaagag
    41221 atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac gctcgtcgtc
    41281 tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca gctgcggttc
    41341 acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca gacgtaaatt
    41401 gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat tttctgtcat
    41461 ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg cgccagtaat
    41521 ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg cagaagccgt
    41581 aggctgacgg ggaggaaaag gtgtaaaagc cataatagag tcctgagttg cttaacggcg
    41641 tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac tacgcgatct
    41701 gtgccttctg cgaggcgact tccaaaaaaa tgaagccatt gaattgttgt gaattaattt
    41761 cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa ggcgaaaaac acgttacaat
    41821 agcggggtac tggattttcg tgagacagga agaagatgga cattaacaac aaagcccgca
    41881 ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg ccgtttttcg
    41941 aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt ctgctggaat
    42001 gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc gatgcagaac
    42061 tggaaatgat ggtccgactc atccagacac ggaaccggga acgtggtcct gtggcaatct
    42121 gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg gctggttgcc
    42181 gctgttattt tactcatgcc ctggccactc agttacaccc cgttatggat ggtgttactt
    42241 tcgctggtgg tgtttgattg cgttcgcagc cagcggcgta ttaatgctcg ccagggggaa
    42301 attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag catcgtcaaa
    42361 gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg cggtaaacgg
    42421 caacatttat ggctggcagc cgacagcatg gacgaagctg aatggcggga tttacggcgg
    42481 attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg cgctaagccg
    42541 tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc accagctctg
    42601 aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac ccacgaacag
    42661 ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg ttggccagta
    42721 gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga gcgcatcgag
    42781 gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaacaa ttttaccttc
    42841 aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga tttcaccaaa
    42901 atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt tcggggagtc
    42961 gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga ttttttctgc
    43021 cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca taacgtaagc
    43081 ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag tcacggcaca
    43141 ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca ggatctggca
    43201 cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga aaatacgttg
    43261 aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg cgggttgacg
    43321 ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct ggagggcggg
    43381 tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt ccagtatctt
    43441 tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc gaaattgccc
    43501 cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca ggcaccatta
    43561 attgatcagc cactggagct acgcgataaa gctatgcttg aagtgttgta tgctaccggg
    43621 ctgcgtgtct ctgaactggt cggactgaca atgagtgata tcagcctgcg tcagggcgta
    43681 gtgcgggtca ttggtaaagg caacaaagag cgcctggtac cgttaggtga agaggcggtt
    43741 tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg tgtgtcaatt
    43801 gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt ctggcaccgc
    43861 attaaacatt atgctgtgct ggcgggtatc gacagcgaaa agctgtcacc gcatgtgttg
    43921 cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt ggtgcagatg
    43981 ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc taccgagcgt
    44041 ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag aacgggaaga
    44101 tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg ttttgctcag
    44161 gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag cagcgatatt
    44221 cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggcgt gttgtacatc
    44281 accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagcgg cacggctccg
    44341 gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa agagatgatc
    44401 gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat tacctgtggt
    44461 tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat caccgtgcgt
    44521 tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat gaaagctatc
    44581 tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa aagcgtcgca
    44641 ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca gcttggcgtt
    44701 agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta ccagccgccg
    44761 aaagagatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa ataattcgcg
    44821 tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac ttgcccgctg
    44881 aattgcctcc cttgctgcgc cgtttatacg ccagccgggg cgtgcgcagt gcgcaagaac
    44941 tggaacgcag tgttaaaggt atgttgccct ggcagcaact gagcggcgtc gaaaaggccg
    45001 ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc ggtgatttcg
    45061 acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg cttggttgca
    45121 gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta agcccggaag
    45181 tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat aacggtattt
    45241 cctcccatgc gggggttgta cacgctcgct cgttgggcat tccggttatt gttaccgatc
    45301 accatttgcc aggcgaaaca ttacccgcgg cggaagcgat cattaaccct aacttgcgcg
    45361 actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat ctgatgctgg
    45421 cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgtggcatc gcaattccta
    45481 acctggcaga actgctggat ctggtcgcgc tgggaacagt ggcggacgtc gtgccgctgg
    45541 acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc ggaaagtgcc
    45601 gtccggggat taaagcgctg ctggaagtgg caaatcgcga tgcgcaaaaa ctcgccgcca
    45661 gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg gacgatatgt
    45721 ccgtcggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg ctggcaaatg
    45781 aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcaa gttgaagccc
    45841 tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct acccggcggg ctggcaatgt
    45901 atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgcatc aaagagcgtt
    45961 ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg tacgctgaaa ggttcaggtc
    46021 gctccattca ggggctgcat atgcgtgatg cactggagcg attagacaca ctctaccctg
    46081 gcatgatact gaagtttggc ggtcatgcga tggcggcggg tttgtcgctg gaagaggata
    46141 aattcgaact ctttcaacaa cggtttggcg aactggttac agagtggctg gacccttcgc
    46201 tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg accatggaag
    46261 tggcgcagtt gctgcgcgat gctggcccgt gggggcagat gttcccggag ccgctgtttg
    46321 acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg aaggtgatgg
    46381 tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc gataccgccc
    46441 tctggccgga taacggcgtg cgtgaagtgc aactggctta taagctcgat atcaacgagt
    46501 ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt tagcgtcatc
    46561 ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg cattttgact
    46621 agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat cgcattcagg
    46681 acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc caagaaagag
    46741 cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga acccgaacgc
    46801 gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac cctcgaccaa
    46861 atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga agctgacgac
    46921 gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa actggcgcag
    46981 cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta cctcgatatt
    47041 caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga gcgtatgtat
    47101 ctgcgctggg ctgaatcgcg tggtttcaaa actgaaatca tcgaagagtc ggaaggtgaa
    47161 gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta cggctggctg
    47221 cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc cggcggtcgt
    47281 cgccacacat cgttcagctc tgcgtttgtt tacccggaag ttgatgatga tattgatatc
    47341 gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc gggcggtcag
    47401 cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccaaccgg gatcgtgacc
    47461 caatgccaga acgaccgttc ccagcacaag aacaaagacc aggccatgaa gcagatgaaa
    47521 gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc gatggaagat
    47581 aacaaatccg acatcggctg gggcagccag attcgttctt atgtccttga tgactcccgc
    47641 attaaagatc tgcgtaccgg ggtagaaacc cgcaacacgc aggccgtgct ggacggcagc
    47701 ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa catgtctgaa
    47761 caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa aacgcgtcgt
    47821 gagaagctgg cgaacctgcg cgagcagggg attgccttcc cgaacgattt ccgtcgcgat
    47881 catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga actggaagcg
    47941 ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat gggtaaagcg
    48001 tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc ccgtgacgat
    48061 ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga catcctcggc
    48121 gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg caccgagctg
    48181 cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt gcaggatcag
    48241 gaagcgcgct atcgtcagcg ttatttggat ctcatctcta acgatgaatc ccgtaacacc
    48301 tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa ccgcggcttt
    48361 atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc gcgtccgttt
    48421 atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc ggaactgtac
    48481 ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg taacttccgt
    48541 aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact ctacatggct
    48601 tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac tctggcacag
    48661 gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt cggtaaaccg
    48721 ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac cgacatggcg
    48781 gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat ccacgttgag
    48841 aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc agaagcacat
    48901 ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct ggcgcgtcgt
    48961 aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg tcgtgaaatc
    49021 ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt cctggatcag
    49081 gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga ttacgttacc
    49141 gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga ccgtatggta
    49201 atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc gatgcgtccg
    49261 gtaaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt tttaattgtg
    49321 ctgcgcgaaa gcagacaaca attttcttgc ttcactattg gctgcctgca tcaccatcca
    49381 cggactgaac gcccacggtg tggcatcaat accgtgtaat acatctgcta aatcacacca
    49441 ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc gtgcggcaaa
    49501 taccggacac acttcatttt ccacaatgcc attcggatcg gtggcgcgat agcgaaagtc
    49561 aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc aacggcggat
    49621 caccgcgtct tcgttgcttt ctcccagttg tgggtgccca caaaccgagt tagtccacac
    49681 gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgtc ctttggcatt
    49741 aaacagccaa ctggagaacg cgcgatgtaa gcgggtgtct gccgtgtgtg cggcatactt
    49801 ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt ccgtttgcat
    49861 aatttctcac atgtaattct gatcactcat cattatagat aattgaagag tgaaatgatt
    49921 aaccaagtga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca ggtaaaaacc
    49981 atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc aacacagtca
    50041 gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc gcgcattaaa
    50101 aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa gccacatacg
    50161 tactgtcctg acttttaaat ctttatcaga gacggattcc ttcaccaggt cagcatgatg
    50221 ctgatagcca ttaaagaaga cattcagcac aacggcgcta agggttgcga gcataatgcc
    50281 gctatgcagc aacggttgca gtacggccgg taacttagaa aagaaatcgt gagagagcgt
    50341 cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac ggttggtggt
    50401 gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga acatcaccag
    50461 cccagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca tttttggcac
    50521 catgccaaac aggattaata taattcccga agaaatacac acccagcggc tatgaacgcg
    50581 cgtcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc tgttaaacgt
    50641 gccgcctatc attgtcccta cgccatcgac acgcagcccg cgaataatat cgtgtgaaga
    50701 gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact cgataaacac
    50761 gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg gcataccaaa
    50821 cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata aattgacttc
    50881 attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg cgacgttgga
    50941 cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg caaaggagat
    51001 acctaaataa acgggattac catattgcgg attcccttta cctccggcgg cccagtcaat
    51061 acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa ccagtggcgg
    51121 gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa aacccgcggc
    51181 gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa tggctatcat
    51241 tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc ccataaagcg
    51301 gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat ccgagctaat
    51361 gagcatcgca atagcttctt ttgagaggcc cagtcggtca ccaatcataa gaggaacagc
    51421 gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca gctttcctgg
    51481 tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg agggaagttg
    51541 ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa agcaaccgtt
    51601 tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc gaggaagggg
    51661 agttagagac aggaattgcg gaggcaatca cataatcgtg gttaaaccac gaaaatgaga
    51721 agagaacctg acaatgaaat tatctgtaat tttctcatat tgatatttca ttaatcattc
    51781 tggcatgtcg cactctcgca tttaatcgtt tttatctgga tagcgctctt ttggtcggca
    51841 atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc agtcgcaaaa
    51901 ttataattaa aacggtcatg ctacctaata tatgcccgtt tattgcatcg cgaaatttga
    51961 gaagacataa ctcattaaat gaaagggatg caaatgaata agtttatcgc tgctgaagct
    52021 gcggaatgta taggctgtca tgcttgtgaa attgcctgtg cggtggcaca caatcaagaa
    52081 aactggccgc tgagtcacag tgactttcga ccgcgtatcc acgttgtagg gaaaggccag
    52141 gctgcgaatc cggtggcctg ccatcactgc aacaatgccc cttgcgttac ggcttgtccg
    52201 gttaatgctc tgactttcca gtccgatagc gtacaactgg acgagcaaaa atgtattggc
    52261 tgtaaaagat gcgcaatcgc ttgccccttt ggcgtcgttg agatggtcga tacgattgcg
    52321 cagaaatgcg acctttgtaa ccagcgcagt tccggcacgc aagcctgtat tgaagtctgc
    52381 ccaacacagg cgttacgact gatggacgat aaagggttac agtggataaa ggtggctcgc
    52441 cagcgcaaaa cggcagcagg aaaagcgtca tcagatgctc agccatctcg cagtgcagcg
    52501 ttgctccccg ttaactcgcg taaaggcgca gataaaattt cagcgagtga acggaaaacc
    52561 cactttggcg aaatatattg cgggctggat ccactgcaag cgacttatga gagtgaccgc
    52621 tgtgtttatt gtgccgaaaa agctaactgc aactggcatt gcccgctgca taacgctatt
    52681 ccggattaca tccgtctggt acaggaagga aagattattg aagcggcaga actttgccac
    52741 cagaccagtt ccttaccaga aatttgcggc agggtatgtc cacaggaccg tctttgtgaa
    52801 ggcgcatgta ctttgaaaga tcactcgggc gcagtctcta tcggtaatct ggaacgctac
    52861 atcaccgata ccgcgctggc gatgggctgg cgtcccgatg tcagcaaagt tgttccccgt
    52921 agcgaaaaag tggcggtgat tggcgctggg ccagcagggt tagggtgtgc tgatattctg
    52981 gcgcgcgcgg gagttcaggt tgatgtcttt gatcgccatc cagaaattgg cggtatgctg
    53041 acttttggca ttcctccttt caaactcgat aaaacggtat taagccagcg gcgagagata
    53101 ttcaccgcaa tgggaatcga ctttcatctt aactgtgaaa ttggccgcga tattaccttt
    53161 agcgatttaa cttctgaata tgatgcagtt ttcatcggcg tggggactta cgggatgatg
    53221 cgagcagatc tgccgcatga agatgcgccc ggtgtcattc aggctctacc gttcctgact
    53281 gcccataccc gccagctcat gggattgccg gagtctgaag agtatccgct gacggacgtg
    53341 gaaggtaagc gagtcgtggt attgggcggt ggcgatacga caatggattg tttgcggact
    53401 tccatccgcc tcaatgccgc cagcgtgacc tgcgcgtatc gtcgtgatga agtcagtatg
    53461 ccgggctcgc gcaaagaggt ggttaatgcg cgcgaggaag gtgtcgagtt tcagttcaat
    53521 gttcaaccgc aatatatcgc ttgtgacgaa gatgggcgtt taactgcggt gggcctgatt
    53581 cgtaccgcca tgggtgagcc ggggccggat ggtcgccgtc gtcctcgtcc ggtagcgggt
    53641 tcagagtttg aattgcccgc cgatgttctg attatggcct ttggtttcca ggcgcatgcc
    53701 atgccgtggt tgcagggcag cggaattaaa ctcgataaat ggggcctgat tcaaaccggt
    53761 gacgtcgggt atttacctac ccagacgcat ctgaaaaaag tctttgctgg tggtgatgca
    53821 gttcatggcg cggatctggt tgtcactgca atggccgcag gaaggcaggc ggcgcgcgat
    53881 atgttaactc tgtttgatac gaaggcatcg tgatgaaatc gttaattatc gttaatccgg
    53941 ctgactgtat tggctgccgt acttgtgaag tagcctgtgt ggtcgctcac ccttcagaac
    54001 aggagttgaa tgccgatgtc tttttacccc ggctgaaggt acagcgactg gacagcatca
    54061 gtgcgccggt gatgtgccat cagtgtgaaa acgccccttg tgttggcgct tgccccgtgg
    54121 gggcgctgac gatgggcgag caggtggtgc agaccaattc tgcccgttgt attggctgtc
    54181 agagttgcgt cagtgcgtgc ccgtttggaa tgatcaccat tcagtcgttg ccgggggata
    54241 cccgacaaca aattgtgaaa tgcgatctct gtgaacagcg ggaagagggg ccagcctgtg
    54301 ttgaatcttg cccgacacag gcgctgcagt tgcttactga aagagaactc aggcgagtcc
    54361 gccagcagcg tattgttgcc agcggtgaga atcctctgtg aaagaaatct ccgcgctgaa
    54421 aggcgcggag aatctattag attgcccaac cacccgcgta aaatgcgacc agtgcggcag
    54481 taatgatcac cgtaccaata ttcaactttt gccattcgcg agcaaagaca cggcctacga
    54541 ccagtgtcac aaagcccagc ataataccgg taacgatatt acaagtcaga acgatgaaca
    54601 cggcacacac caggccagcc attgcgtcaa taaaatcgtt gaaatccagc ttcgagacgt
    54661 tacttaacat cagcaaaccc acgtacatca gagcgggtgc agtggcgtaa ccagggatca
    54721 aaaatgacag cggtgataag aacagaatca gcaggaataa cgcccccact acggttgcgg
    54781 tcaaccctgt tttacccccg gcggcggttc ctgccgctga ttcgatataa accgctgcgg
    54841 gcgctgcgcc caccagaccg gagaatattg aacttactga gtcactggtc agggctttgc
    54901 cgccattgat gatctggttg tctttatcca acaaattcgc ctgaccggcg acggcacgga
    54961 tggtgccagt ggcgtcgaac actgcggtca tcaccaatgc cagtacactc ggaagtacgg
    55021 ttggctggag tgcgcccata atatcgaggc tgaaaatcag agacttacca tcttcgccag
    55081 tcagacttgg catcgccacc agaccgtggt atttcaccgc tgggtcaaag attaagccga
    55141 taatcgaaat tgcgataatc accaacaaga tcccgccggg tacgcggcac ttctccagac
    55201 cgaagatgac cgccagcccc agcaagctca tcatcaccgg gaaggaggta aacgcaccga
    55261 gcgccactgg caagccttca atcgggtttt tgataaccat acccacaccg ttagcagcaa
    55321 tcagcagcag aaacaggccg ataccgatac ctgtaccgtg agcgataccc atcggtaaat
    55381 tacgtaagat ccaggtacgc acaccggtta cggaaatagc ggtgaagatg acgcccatca
    55441 gaaagaccgc gcccagtgcg acaggaacgc taatttgttg cccgagtacc agactgaatg
    55501 cggtaaatgc cgtcaaggaa atcgcacaac caatcgccat tggcaaattg gcccacaacc
    55561 ccatcagcaa cgagccgaag cccgcgacca gacaggtggc aacaaacaca gctgcgggag
    55621 gaaaacctgc tttgcccagc attcccggaa cgacgataac ggaataaacc atggccagaa
    55681 aggtcgttaa gccagccagt acttcctgac gaacggtact gccacgagcg gtaattttaa
    55741 aataattatc cagcgcgccc tgtggctttg gtgcgtccgg tgtttgtagg atgtctccag
    55801 acataatgat gtcctcacga ggatgttaaa gttttattat tagttgcgtt cgtacaccag
    55861 acgaccatca acgtaggtgc ggtagatcga acggtcatcg cccaacgtca tcatcacgaa
    55921 caatttgtcg actaaagaaa cagagttgtc atagcgcagc tgctgtagcg gagtggcggt
    55981 gggttccatc accacgaaat cagcctcttt gccaggtaaa aagttgccaa tcaaatcgtc
    56041 aaggcccaga gatttcgctc cgccgagcgt ggccaggtaa aacgcttcat atgccgagag
    56101 gcgatagcct tgtaattgca atactttgta ggcttcgttc agcgtttgca gcatgttgaa
    56161 agtggttccg gcaccgatat ccgttcccat gcccacttta actttcttct gccatgcttt
    56221 tttcaagttg aataagccgc tgccgaggta aaggttggag gtcggacaga aagcaatgct
    56281 ggatttggtt tcgctgagac gatcccactc tttttcttcg agatggacgc agtgagcaaa
    56341 gacacagttt ttaccggtca ggccgtactg atggtaaaca tccagataac catcatggtc
    56401 aggataaagc gatttcaccc aggcaatttc atctttgttt tcacagagat gggtatgtac
    56461 ccacgtatcc ggatattctt ctttcaggcg ttgcgccatc gccatctgtt caggagatga
    56521 ggtcggggcg aagcgtggcg taatcgcata tagcagacga ccatttttgt gccagcgttc
    56581 aatcagttct ttgctttggt gatagctgct ttcggcagtg tcgagcagat aatccggtgc
    56641 gttgcggtcc atcatcacct taccggcaat catacgcata ttgatatgac tggcggcttc
    56701 aaacagcgca tcaacagatt gcggatgaac agtgccaaac accagcgccg tggtggttcc
    56761 gttacgtaaa agctgcttga tgaagaatgc cgacatttcg cgggcgtact ctaaatcctc
    56821 ataacgacgt tcagtaggga aggtgtgttt attcaaccac tccagcaatt gttcaccata
    56881 ggcccccacc atttcacttt gcggataatg gatatgtgta tcgacaaagc ccggtactat
    56941 cagtttgccg cgatagtcgc gcacgcgaat agtgtcagga atttgatgct ttccgtcttc
    57001 ccattcgcca aaccattcca cttttccctg tttaatgagt aataaaccat cctcaataaa
    57061 ccgcagcgca gaggcaatct cttccgggtt atcgaccgta cgggtgacat caataaaact
    57121 gcctcgtacc gctttcaacg tgtgttctcc tgacatcatt gactccttta ctgttaatcc
    57181 atctcttccg ctgaggtaat accaggcaga acgttttcct gtcggtagcc accagggagg
    57241 ataatattta ataaaatcgc agttaacccg ccagcacaaa tagggttttc aactagtaca
    57301 taaatagagg ctggcaatat tttaaaaatt tcgggatcgt aggagacgcc aagtcctaaa
    57361 cctaaagaag tggcgacaat aagtgtttct cggcgcttta aaccgttggt gatgattatg
    57421 cgaatcccag cgatggcaat catggaaaac atcaacgtca ttgcgcctcc cagaactgcc
    57481 gagggaatgg tcgtgaagaa gccgccaatc atcggaaaca aaccgaggat aaccagcatt
    57541 accgcgatgg ttcgcccgac ataacgtgaa gcgacgccag tcatctgaat aaccccatta
    57601 ttttgcgcaa acgtggttaa gggtaatgac ccgacagcgg aggcgataac agaaaccaga
    57661 ccatctgcca gcacgccgcc tttcagtcgg gactgatact cttccccctg aatggggcgg
    57721 cgggaaacca ttgccgtggc ggtgatatcg ccgactgctt ccagcacgct aagcagataa
    57781 atcgtgccaa ccaccaggaa ctgatggaag ctaaaactaa agccgtattt gaacggatgc
    57841 gggatggtga ttaacggcaa attgcgcata ctgctgaaat ccaccatgcc caggcataac
    57901 gatgcgatat agccgacaca tagcccaatg gcgatccctc ccatgcgtag caacggactg
    57961 cgacagcagt taaagccgat caccacaatt aaaaccaata aaccaacgcc gagatgttcg
    58021 taattgccga acgtaccgct gcttttggct gcaaatcctc caccaaaatc gataatgccg
    58081 actttaatca ggcttaagcc gatcatcagt accacaatac cgctgacggt aggcgtaata
    58141 acccgacgta aatagggcaa gataaatgaa gatccgacaa ccagaaatgc gccaacgaag
    58201 gagacgccga gaagcgacga catgattaac tcttcgtgaa aaccgtcgct tttcatgctg
    58261 ctgcccagcg caatcatgac cgtaacaaat gaaaaattga ctgactgaat tgagagtagg
    58321 ccagaaccga cgatgccgta gcggtttact tgtaaccagg taccaatacc agaggcgatc
    58381 atcgccatag aaacaagata ggcggttgtt tcagcggaaa gctgtaaggc cgcacccacg
    58441 attaacgcgg gggttaccat cggaacgaaa attgccaaca gatgggtaat ggcaccaacg
    58501 agagcctgat gaaagggagg gcgatcctcc agttcaaata taaggtcaga acctgcatgg
    58561 tttatatcag acatcccttc actcctttta aagtaaattt gatcatctga attagtaaaa
    58621 taaattccca cgaatttggg gcgcaggacg ccttgagaac ggcttatctc attttcgcag
    58681 tcactattta gtcatcgttc aaacagagaa acaggccgcc gaagcggcca tttattactt
    58741 atattttttc cagcgcggtg agtattttct ccggtgtgaa atgccattcg cgtaaccaga
    58801 tgccgcaggc atcgtgaatt gcggtagcaa tcgccggagc tgcgccattt acaccaattt
    58861 ccgagatcga tttcgccccg aacgggccga ctttatcgtc gctcggcacc aacacagcgc
    58921 ggaaatcacg cggaatgtca ccaattttcg gtgcgccgta actgcgtaaa tcacgcgtta
    58981 acgggtgacc ttcggcgtta taaatgatct cttcgctcat actgtggccg atagcacgca
    59041 gggtggcacc gtagatttgt cccaacgcta actctggatt gaccggtgta ccgcagtcca
    59101 gcaaggcgta gaatttatcc aggcggattt cacccgtacg cgtgttgacg gcaacttcag
    59161 cgaagtttgc gccatacggg aaagcgaaat caggcgtgat ataactgcca gttcccacca
    59221 gtgaaccaaa gccggtgccg gtttcgcctt tatgggcaat atccccgaaa ctaacttcgc
    59281 ctttcttgcc gcgcacgacg cccggcgttg ctagttgaac atctgccact ggctcaccca
    59341 acatttgcgc gccgtggaac agaattttct cccgcagatt ttccgctgcc aaacgcgccg
    59401 cgttacccga gaagcaagta ccggacgagg catatgcgcc tttatcaaac aacgcatgat
    59461 cggtatcacc ggagataaca tgcacgtcct gcggtgggca gtgcagcact tctgctgcca
    59521 gtttcgtcac tacagtatcc agaccagtac caatatccgc accgccagaa tgaacgataa
    59581 aggtgccgtc cgattccagt ttgatcatgc agttagcctg atcgatatcc gggatacccg
    59641 atttctgcat gataatggcg acgccgcgac cgatgtgcca gtcactattt tgcggtttcg
    59701 gtgaagacca ttggatcatc tcgcgaccct gacgcaggat ctcttccagt gcgcagctgg
    59761 cggcggaagg aacggaggtc ggcgctttac cttcaccgat tgcaccgaga attttcagct
    59821 cttgcccttc gtgtacccgg ttacgttcga taatttccag ttggtcgatc tgtaactgtt
    59881 cagccagttc cgctaatgcc atggtgatag cgaagttacc tttcggtgcg ccataaccct
    59941 gataagcacc atttgggcaa atgttgctgt agtaggtggt gacctggaaa tcgacgttat
    60001 cgcacggata taacggcagc gacagcgccg gtccgttaca cggtacggtg agtgagtggt
    60061 tgccgtaagg gccagtgttg gcgcggaaat ccatcttcac tgccgtcagg cgaccatctt
    60121 ttttcgctcc cagtttgacg gtgactttcg cgacgtgacg agaggtgtta gcaataaact
    60181 cttcttcacg ggtgtagcgg aacagtaccg gacgcccggt cacgcaggtt gcccatgcgc
    60241 acacttcttc cagcaggatg tcctgtttgg agccaaaacc gccgccaact cgctctttaa
    60301 tgacatgaac tttatgctgt ttcatgccta cgaggcgcgc gacctggcgg cgtaagtgcc
    60361 atggtacctg ggtggaggcg tggataacca gacgatcgcc gtccatccgg gtaaagcaga
    60421 tatgtgtttc agtcgggcac tgctgtgcct gcgttgagtt ataggttcgc tcaatgatca
    60481 catcggcatc ggcaaagcct ttgtccatat cgccaatatg accatgaata ctggcggcga
    60541 tatttttgcg agggcgagaa ccgatcggga agttgatgat catatgctcg ccgcgctggg
    60601 ctgcatggct gttatcgtct tccagagtat ctggcgcacc agcaacatac accaccggtt
    60661 catcgtgcac gacaggcgca tcttccgcca ttgcttcgtc gatcgacatt accggcttaa
    60721 gcacttcata ttcaacgtcg atgagcttca atgcttcgag cgcaatttct tcactttctg
    60781 cgacgaccgc agcaacacga tcgccgacgt gacgcatttt cttgccgaac atacggcggt
    60841 caagcggtga cggttccggt gcgctctgac cacccggggt atagtagata tccgggcaat
    60901 tcaggtgagt aataacgtga acgacgcccg gtaaggcttc agctttgctg acatccagat
    60961 gagtaatcag tgcgtgagcg tgtgggctac gtaacatttt aatgacgcag gcgtcagccg
    61021 ttacgcggtc ttcaacatag cagggtttcg cctgcaccat tttcgcggca tcagttttag
    61081 gataatgctt gccaatgact tcaaggtcgt cacggaaagt cggagcgata tcaatggtgg
    61141 cctgcggatt atttttacgt gcaaccgcca gttcaatgac ctgatagtat tgttgccagc
    61201 ctgcatcgcg gctgaacaaa ccagaaagcg cgtcgtcgat ctcttcgcgg gtaggtgcgg
    61261 cgatgcgatc gagaagatcg gtgataatca gagctgcagc tggatcgtta taaccagact
    61321 gcaccacgcc aacatcaacc attgcctgtt gaaccagact taactcgttc catttgccca
    61381 aagattctgc ggtacgaata tctgccttct ctaactgtgc ggcaataagc aaggacgcgt
    61441 taacgatatt accgttaaag attattgcgt cagaaccggc aaacccgaag ccatcatcac
    61501 tgttgcgtac agagtgcatt cccatgttaa acaacagctt ttgcacgttt tcgcctggat
    61561 taacggttag ctcctgaggc gcgccattta aagtaaagtg gatgatcata cggcttcctc
    61621 cccagcctgt tggcagtcgg catacaggtc ggctaccaga actcccgtga tatagcgttt
    61681 ataggccacg ctgccccgca aatcttcctg cgggaaaatg gcgttggcga cagcctgttc
    61741 aagtgcattg ccttccagat tttgtttttc gacatcatgc agacgcagtg ctttactggc
    61801 gacgccatcc agcgcaattc gcataccgtc gtggtctgtc attgcaacgg ctgccgtcac
    61861 gacggttaaa cctgcctgag aacggctaat tttgcgggtc gcacaggtgc gatacggatc
    61921 tttaatgata atttcggtta gcaggcgatc gcatgggcag gccaggtagt cctcgattga
    61981 cagcgtttcg ccgttgccaa aaaccagttc agcatccagt gccaacagga cgggaagcag
    62041 caccgactct tcctggcggg cggcgatttc accaccaatg gtcgactgat tacgaacatg
    62101 gcgtgagtaa acaaaaccga gggcttcacg cagcgctgca ggaataaatc gcgcatcacg
    62161 cagtggctgc aagcgagaca ttgcgccaat ccgcagtgca ccgttatccc agtcaaccca
    62221 gtccagttcc agatcctgta aggaaatggc aatcttttta tcggtacggg ttggtgtagc
    62281 attgagtttg ctgcccccgg cgaaccagac ggcttcatcc tggtagcggc gcttcagttc
    62341 cagcgcctgt tcgacagagt cgggcctgaa aaattgttca atcatcttct ttccttattt
    62401 aaccctcttc cctgcgggaa gagggcatct gtcatttatg ccagcgcatc catccgacgc
    62461 cacatactgg cagcggcttt tctggcttgt gcataaatgg aatcgcaatc gaagttaaac
    62521 tgacggtctt catagaccat cacaccattg accatcacgc tgtgaacgct gcctgagccc
    62581 ataccgaaag cgatatgccc ggcgatattg tctgccagca gcggcgtcgg tgagttgtaa
    62641 tcgcaaatgg ttaaatcagc tttgtaaccg gcttccagaa gtccaaattt cgcgccaaag
    62701 ttgcggctca tcagttcgtt accgttagtc agggctttgg caaaactgtc aggccacagc
    62761 ggaccacccg catcgcgatg tttaaagaag gcaaatttca tctcttcaaa catgtccgaa
    62821 ccaatgccgt ccgttcccaa cgccagattg cggatgtcgc taaggtgatg gttgtagccg
    62881 acatggttgt tcatgtttga acgggcgtta tgcaccagga acgcatcgcg ctgattgagt
    62941 agggcgatgt catctttcga caagtacagc ccatgagcga ccagcgtttt gctatcgatg
    63001 agatcgaatt gcgctagtcg tgccagcagg tctttgccgt accagtggtg actgtaggaa
    63061 acgtcgtaaa ggtcttccgc agcgtgaata tgcaaaccac ggcctgtggc tttcacggct
    63121 tcacgtagca tctccagacc ggcatctggc acggtaaacg gcgcgtgagc accgatatgt
    63181 gcttccacca gatacggctc ggttgctgct ttcttcgcct catcaatctg acgggcgaag
    63241 cggatgtttt cttctacacc ttcctgcaac tctttgatgc cgttgttacg gtcagtagtt
    63301 tcaaaacagg tcatcgcgcg caggccaact tttaaaaatg cgtcgcgcaa tgtggagagc
    63361 gacccgccga tatacgccgg agaggcatgg tgatcgataa ccgatgtaca tccgctctta
    63421 atcgcttcca gggaacaaat cagcccgctg taatagagcg actcttcatc aagggcgcga
    63481 tcgagccgcc accagagatt tttcagcgtt gagatgaaat ccgggcaggg ggcgatgttt
    63541 gccataattc cgcgggaaag ccccgagtaa aaatggttgt gcgagcagac aattcccggc
    63601 atcacaatcc gaccatgcat ctctttgtag ctggcatcgg ggtagcgttg cgtcagggca
    63661 tcgccgatag cgacaatcac atcattttcg atagcgatat caacgccttc ctgcactttc
    63721 gccgggtgta actgcactgc ggtgacattc ttcagaatca acatgattac acctccacgc
    63781 ggcccagcag ataatgatga tgctggtgga catggctgat gatgcggcac atatcgttca
    63841 gctccggtgg tacgttgttg aactgacctt cgctgtcgat gtttaacacc cagctttggt
    63901 tattcagtcg tactcgtacc cggcaatctt ccacaaggaa gcctgggttg ctgctgttat
    63961 caaagtcttg cgccaggctg aaaacggtga ttttgtcttt gtatggttta ccgttccacg
    64021 gacagaactg agcgcagttg ccgcattcgt tacagtaagc gtcgaggtgc agcgtctgga
    64081 atctattctg gaagcctggg accgcaatgg agacgttggc gcggttcggg cagacatcca
    64141 cacacttgct gcaaacgtag ttacattcga ggcagcgagc ggcttcctgg gcgacaaacg
    64201 cgtcacgatc gtcactgttc accagagtga tagagatatc gcctttacgt tgatagattt
    64261 ccgccggatt gacgttgttc cagtatttat cgttctggtg ggaacggata ttttcccgac
    64321 taaggatggc atcggtcgcc cgacgcgcgg ttccgacagc agcgacaatg gaggatggtc
    64381 cgcgctgtac gtcgccgatc ataaagacgt cagtcagacg agtttcgcca ttgtggtcga
    64441 cgtctggcca gccgtttttg tccagcggca cgcccatcgc attcagggct tcagtatcct
    64501 gctgttcacc aatggcggtg atcaggctgt ctacatgcag tgttactgtt tcgttggttt
    64561 caaccggacg gcgacggcct ttctcatcgg gttcgccaag cgacataacg cgcaaggtta
    64621 aggtgccatc agcatcgaaa cgttccggat tattcaggaa acggaactcc acgccgtcgt
    64681 gcaacgcttc ttcatactct tcgcgccatg cgggcatctc ttgcagtgaa cgacggtaaa
    64741 cgatcgttgc tttttctacg cctggaacgc gtaacgccgc acgagcgcag tccattgcgg
    64801 tgttacccgc cccgacaacg accacatgtt tgcccagctt gagcgctgta cccttgttgt
    64861 attcacgcag gaaggggagt gatttccaaa cattttggtt gtcacccgcc agtttcacac
    64921 cgctattttt atcagtgccg gtggcaatca gaacatagtg gaagccctga ttttttaact
    64981 gttcaacggt caaatcgggt gagcagccat actcaaattt cacgccgtga tcggcaacaa
    65041 aatcgatatc gtgctgaatt aactcagccg gaattcggaa ctgaggaatg atatttttca
    65101 ccacgccgcc cgcattggct tcgcgttcaa acagcgtaac cggatggccc gctctggcaa
    65161 ggaagtaacc tgctgccaga cccgccggac ctgcaccaat cacggcaacc ggatggcgtg
    65221 aaccagaacc ggctggtttg tgccagcgtt gcttatattc atcccaacct ttttccagcg
    65281 cgactttttt cagttcgcgg atattcagcg cactgtcgta atccaggcgg gtacagttgt
    65341 attggcactg gtgatcgcaa atatgaccgg taatggcggg cagagcgttg cgttggtaga
    65401 tgagttccag cgcgtcggca tagcggtgtt cgccaagcag acggatgtat tccggaatat
    65461 cttgcttgat agcgcaggca gtaacacagg gggcaacgta gcaatcggtc agcggcaggt
    65521 cttctgccac ttcaatacgc tcttctggct tccagtgttt ctgggtgtat tccatggtta
    65581 acgcatctgc tgccagtctg ttcagtcgtt cgacgtcaac atggtcaagt ccccaggcgt
    65641 cggagccttc cagctcgcgc atgcaggcac ttaagcgcaa atagccgcca ggtttcagca
    65701 ggtcggttgc catagtaata gggcgaatac ctgtatcaaa aatatcgcgg atagtcagct
    65761 gactggcacc accggaataa gaaatgggca gtttgccgtc aaaggcgcga gagagaactg
    65821 ctgcaacatt gatggagagc gggaacagcg cacggcctga catatacatc tcttcaccag
    65881 gcagtgcgcc tttattgttg atggtgccga gagtgttagt cagttttacg ccaaagccga
    65941 gtgatttttc ttttgccagt gccatcaggc gttccagcat ttccagtgct tgcgtcagct
    66001 tgaggtcgtg atcaaatgac tcttctttta agcctatgta accgaaaccg cagacatcga
    66061 ggatctcacg aacacgcgcg tatgccagta aggtcgggtt aagtttcaca aaggtgttga
    66121 gccctttttc ttccagcatg tagcggcaaa tggcttcgat ttcatgcgga ggacagccgt
    66181 gcatggtgga gagggtgacg ccctgcacca tactggtggg gatgcgagcg ggtaaggctt
    66241 gcaagctttc gcgtttttcc tgcaatccgt ggcgggctaa aaatgcgtca tcctggagta
    66301 atttattcag cgtatcgcga tattgcgcga atttcggatg gtcagatgcg tccatcatat
    66361 tgtcgatgaa ctgttgcatt ggcggttgct taataccttc gaggttgtaa ccgacgctca
    66421 tattaaagat gaacgattta ccggaatcag aaggctggaa catcgcttcg agaaggtgca
    66481 gggcaaacca ggctttgagg tattcatccc aggctttaag cagggtaaat tcggtagacc
    66541 attcggtgtt aaagcactcg tcttcggcat cgatacaggg cttttccagc tccaggcggt
    66601 caagaatttg gacggttttt agttcgatga agcgtccgcc agtcagccag gaagtgacaa
    66661 tattttgcgc gagctgcgtg tgcgggccag cggcagggcc gacgggagtg gcacaggttt
    66721 caccgaagac gctaacagtt ttacctttta caggtgagta aaactgttgc tcgggaatac
    66781 caaagattga gcgttgttgt tggtattcat caaatatgcg cgtcaaaagt tcctcaaacg
    66841 gaatgggacg cataatatcc cccataaccc tctccttagc ttatacagat atcattgagt
    66901 gaccggtgtt gagaaatccg gtggtttagg gggatagagc aacaatcgca ccactcttgc
    66961 caggtttggc gggaaatata aaaaaatgtg aggcggctcg aaacttaaag gctttttgtg
    67021 aatgaactca caggtgccaa atgagaatta aaatcactcc tgaaaattat tattgtcagc
    67081 ccatttatca ctttttctct tttctcaaaa tcagtgattc cttatcatta tgataaagag
    67141 tgctggagag attaaatatt tgaacgcaat cgtttaacta cattttctaa attttagtat
    67201 accttttttg cgctgtaata aagtcgtccg gagtatcaat atctaaaatt atttctgcat
    67261 tttctatctc gacggaataa tgagctccca taaggagtgc ctgacgcata ttggtgacat
    67321 tgggtcgttg gattgcctgc atcaagcatg gttttgacac taaaatcgga tggccgggga
    67381 tgccattatg gagtggcagt attgcgccat catttcgtaa cgaccagatt tttctaaaaa
    67441 tatcgatggt gagggttggc atgtcaccgt gggtgagaaa acaatgttct gtttgcactg
    67501 cgggtactgc ggcctttact gatgttagta aaccctgcgc ataatctggg ttgtggataa
    67561 tagtgatatt gctctggttc gcataacgtt cgtgcagttc attaccacga tagccggtga
    67621 ctaaaataat tcggctacaa aactgcaacg catttttgat acttgtatca agaattgtcc
    67681 cctgttgcca gggtaacatc attttccatt gccccattct tgatgataat ccggcagcgg
    67741 taattataca gtcgatggct gacatagtgg ttccattatt tataggggct aataacgcct
    67801 gataattgcg ttacttcctg atggattaag ttggggataa tttatcccag agaggtcata
    67861 aagactcgtg aaaagtatag ttgacccatc ggcattagtc attgatttag gtgcgcagaa
    67921 acgtcccact gtgatttctg ttgttggtgc tggggggaaa accagcttgc ttttttggct
    67981 ggcagaactg ctccaggcta gcggcaggcg tgtgttaatc acgacgacga cacatatgtt
    68041 tatgccaacg tctcactggc ccgtggtttt ctgtcgtgat cccgccatgc ttcctcatgc
    68101 gtctcttaca tcccccattt cattttgttt tcacagctgg aaagcgaacc agggaaaagt
    68161 gcagggattt acgccagaag cgattgatgc actggtgcaa cgaccagagt gtgacgtaat
    68221 tctcattgaa gcagatggct cgcgaggaat gccgttaaaa gcgcctgatg agcacgaacc
    68281 ttgcataccc aaaagcagtt gctgcgtgat tgctgtgatg ggaggacata ctttgggcgc
    68341 gaaagtgagc acagaaaatg tccatcgctg gtcgcagttt gctgatatta ccgggttaac
    68401 acctgatgca accttgcaac tgagcgatct cgttgcgctg gttcgccacc ctcagggggc
    68461 gtttaaaaac gtaccgcaag gttgtcggcg agtctggttc attaaccgtt tttctcaatg
    68521 tgagaatgcg attgcacaaa gcgagctcct tcaaccgctg caacaacacg acgtagaggc
    68581 aatctggctg ggcgatatac aagaacatcc tgcaatcgcg cgcagatttg tgaattagcg
    68641 gcccgggtat tccgggttac tgaaaggata tccattgagg ttggtatgaa tattttcaca
    68701 gaggctgcaa aactcgaaga gcaaaattgt ccgtttgcga tggcgcaaat tgttgatagc
    68761 cgaggctcga ctccccgcca ttctgcacaa atgttagtgc gcgccgatgg ttctatcgtc
    68821 ggtacaattg gtggcggaat ggttgagcgg aaggtgattg aagagtcact tcaggcattg
    68881 caggaacgta agccgcgatt attccatgga cgtatggctc gtaacggtgc ggatgctgtc
    68941 gggtcagatt gtggaggtgc aatgtcagta tttatcagcg tccatggtat gcgtccacgt
    69001 ctggtgttga ttggcgcggg gcatgtcaac cgggcgatag cccagagtgc ggcgctatta
    69061 ggatttgata ttgccgttgc cgatatttat cgcgaaagcc tcaatcctga actattccct
    69121 ccatcaacca cgcttctcca tgctgagtcg tttggtgcag cagtggaagc actggatatt
    69181 cgccctgata attttgtcct gattgccacg aataatcagg atcgtgaagc cctcgacaaa
    69241 ctcattgaac agcccattgc atggttgggg ttgctggcaa gtcgccgcaa ggttcagctt
    69301 ttcctgcgtc aattgcgtga gaaaggcgtg gctgaagaac atattgcccg tttacatgcg
    69361 cccgttggtt acaacatagg tgcggaaacg ccgcaggaga tcgccatcag cgtgctggca
    69421 gaaatattac aggtgaaaaa taacgcgccg ggtgggctga tgatgaaacc ttctcatcct
    69481 tccggacacc agctggtggt gattcgcggt gcgggagata tcgccagtgg tgtggcgcta
    69541 cgtctgtatc atgcgggttt taaagtgatc atgctggaag tggaaaaacc gacagtgatt
    69601 cgttgtaccg tggcgtttgc ccaggccgtg ttcgatggcg aaatgacggt cgaaggcgtt
    69661 actgctcgcc tggcaaccag ctctgcggaa gcgatgaaac ttaccgaacg cggattcatc
    69721 cctgtgatgg cagatcccgc ctgttcattg cttgatgaac tgaaaccgct ttgcgtggtg
    69781 gacgctattc tggcgaaaca gaatttggga acgcgggcag atatggcacc agtaacaatc
    69841 gcgcttgggc cgggctttac tgcagggaag gattgtcatg cggtaattga aacaaatcgc
    69901 gggcactggc tcggtcaggt gatttactct ggttgtgcgc aggagaatac cggtgttcct
    69961 ggcaatatta tggggcatac cacccgacgg gtgatccgtg ctcctgctgc aggcattatg
    70021 cgatccaacg tgaaattagg cgatctggtg aaagagggcg atgtgattgc ctggattggt
    70081 gagcatgaaa ttaaagcacc gttgacgggg atggtgcgtg gcttgttgaa cgacggcctg
    70141 gcagtggtcg gtggttttaa aattggtgat atcgatcctc gtggtgaaac ggctgatttc
    70201 accagcgttt ctgataaagc ccgggcgatt ggcggcggcg tacttgaggc gttaatgatg
    70261 ttgatgcatc agggcgtgaa agcgacaaaa gaagtgctgg aagtggctta aatgaaaatg
    70321 gccggatggt attacccacc cggccttaaa attactgaca aatcaccgtt ccggttttgc
    70381 cttcaatccc ctctttcgct ttgctcaata cggtaatcag tgcttcacga cctgcgcgag
    70441 agcgagcaaa cgaggcggca gcttccactt taggcagcat agaacctttc gcaaaatgac
    70501 cttcttcgat aaagcgttca gcatcactta atgacaggcg atcgagccac tgttcattct
    70561 ctttaccgaa gttaatggcc actttttcta ccgcagtcag aatgatcagc atatcggcat
    70621 caatcatttc tgctaaacgg gcgctggccc agtctttatc gataaccgcg ctggcaccac
    70681 gcagatggtt accttcacga ataacaggaa taccaccacc gccaacggtg atcaccacct
    70741 ggcctgcatc taccagagct ttaaccgttt ctttttcaat aatatcaacc ggttttggcg
    70801 aggcaacaac acggcgatag ccgcgaccag catcttcttt cagagtgtaa ccctgttttg
    70861 tcagttgctc tgcttcttgc tcggtaaaga acgaaccgat cggcttggtc gggttgagga
    70921 atgccggatc gttagcgtcg acttcgacct gagtaaccag cgttgctacc ggtttattaa
    70981 tgccccgaga aagcagttct tctcttaagg cgttttgtag atcgtaacca atataaccct
    71041 ggcttaaggc aacgcagaca gacatcggca gcattggcga gtgcgcttca gtttttgcag
    71101 cagcttcaaa ggcctggtta atcatgccca cctgcggccc gttaccatga gtgacgataa
    71161 cttcatgtcc ttgggcaatt aaatcaacaa ttgcctgaga agtaattttt accgctttca
    71221 tttgtccggc caggtcatcg cccagcgcat tcccgcccag ggcgagaaca attttcttac
    71281 tcatttttcc tcactcatca ttcttgattg tgtatagatc cctgacagtg gttagagcac
    71341 gggagggaca aacggtttgc gtcgcaggaa acgacctcgc ccggctttgc ctgtaaaggt
    71401 gccgtcacag aaaatcgttt caccacggga taaggttctg acaatcgcgc cctgacaggt
    71461 aaaaccctcc catggcgagt agtcggcgtt gtcgtggaga tggcgatgct gaatttgttg
    71521 gctctgacgt gggtcgataa tcaccacgtc gccatcggaa ccgggcgcta ataatccttt
    71581 ttgcggccac aggccaaaca gcctggcggg cattgcgctg gttaattcaa caaagcgttc
    71641 cggtgttata cgtcccgtca tcacgccact ggaaaacagt aactgcatgc ggttttccac
    71701 accgggtaag ccatttgggc agcgactgaa atcgccttta gaaatttgca ggcgttgagc
    71761 catcgagaag gtgcagtgat cggtcgccac cacgtcaatc gcaccatcgc tgatgccaca
    71821 ccacagtttg tcctgctcgc gtacgttacg cagcggtggg ctaagaatga acttcatgcc
    71881 gtcttctgta tcgtaactgc gttcgtccaa caggagatat tgtgggcagg tttcaaccca
    71941 gactggctgg tgattcgcac gggcaagacg cagataatcc agacctaagc cgttagacag
    72001 gtgcacgata tagagcgggg cgttaccggc aatttgtgcc aggttaatca tgcgggcgat
    72061 ggcttccgct tcgcattcca gagggcgact caaggcatga tagcgcggcg cggttaaccc
    72121 tgcggcgata aattccgccc gcttgctggc gatagccgca tcattttccg ggtgcacggt
    72181 ggtcagcgcg ccggattcat gcagacggcg taatgcctgc aaaacctcgt catcgttgag
    72241 tttgtattga taggttaaat agagtttaaa actgctcagt ccttcctcga ccatcatcgg
    72301 gatttcgtcg aggattgcgt gattaatgtg ctggatcaca ccgtgaaagc tgtaatcgat
    72361 gaccgcttta tgggcggcat aaccacgata aacctccagt tgatggcgta accgacagcc
    72421 gtttgggcca aatcccatat ggtcaataat ggttgttgta ccgccacacg cagctgcgcg
    72481 ggtaccggta aaaaaatcat cacaactgcg cgcgatgccg acatcaatat tgaaatgcgt
    72541 atggacatcc acgccgccag ggaaaacgta acagccagtg gcatcaattt cttcatacgg
    72601 gagctgcggc gaaatattgt tgcccaactg gcgaacaatc ccgctttcaa tcagcaaatc
    72661 ctgtttggct tgtccatctg cgttaacgac agtgccgttt ttgatcaata cgcgcatagc
    72721 aaactccaaa aaccctccgg cgaaccggag gggaataagt gaagagtaat tactctgttg
    72781 ccagccagct taacgggatt gcagcgtaca tcgcggcaca ggtcaccagg tgagatttcc
    72841 aggttttttc gttaggtgca tgcgcttcag gttctttacc cgggccaaag ccgatcaccg
    72901 gaatgccgtg acggcccatg atagatacgc cgttagttga gaaggtccac ttatcaacaa
    72961 ccggcgcttt gccaaacagc ccttcgtagg cattcaccag tgctttaacg gtgaagtgat
    73021 cttcttccac tttccaggtc gggaagtagc attcggttgg gtaaaccagg ccagtccagg
    73081 acggacggtc gtagttgtac atagaaacaa ccgcgttagc tttctgtact gcaggcaggg
    73141 cgcggatttc gtccagcgcg ccttcccagg tttcgcccca ggtcagacgg cggtcaatag
    73201 agacggcgca gctatctgct acagcgcaac ggcttgggga tgtgaagaag atttcagaaa
    73261 cggtgagggt gcctttgccg aggaattcgt cataacccag acgttgggag agttcttgta
    73321 attcgccaag aatcggaccc attttgaaaa tggcgttgtc accgcgttct ggcgcagaac
    73381 cgtggcagct aacaccctga acatcaatac gaatttccat acgaccgcgc tgaccacggt
    73441 atatctggca gtcggttggt tcggtactga ccacaaattc cgggcgaatg ccggattgtt
    73501 caataatgta ctgccagcac agaccgtcgc agtcttcttc ctgcacagta ccggtaacca
    73561 gcagggtata ttcatcttcc agaccgaggt ctttaatgat tttaccggca taaaccatag
    73621 atgccatgcc gccttcctgg tcggaagtac cgcgaccacc gattagctca tcagtttcca
    73681 tgccttcgta cggatcgaag tcccagtttt tgatgttgcc aatgccgacg gtatcgatat
    73741 gagcgtccat tgccaccaga cgcgggccgt ggccgatata accgagaacg ttgcccatcg
    73801 ggtcgatttc aactttatcg aagccgactt tttccatctc ttctttaata cgatgtacta
    73861 cgcgtttttc gtcgcagctt tcactgggaa tagcaaccat gtcgcgcagg aagcgagtca
    73921 tgtccgcctg gtaatctttt gctttttcaa gaatcagttt gaatggaata ttcttagcca
    73981 ttatttttct ccagattttt tactccccgt tttgcaggga gtagttccaa tagagtgatt
    74041 aaggtgctac agcgtgtttg ccttcccaga caacttcgcg gtagtgcttc acgtcggtgt
    74101 cgccttcagt gctgataacc agcaccacgg catctttgtt cagcgccagt ttttccatca
    74161 ggctttgacg ttgcgggtga taatgaaccg ctgcgagaac gcccaaaccg acagcgccgg
    74221 attcaccgga gatgatgcgc gggtcgttgc cgtacggatt acccagcacg cgcataccta
    74281 atgcggcaac gctgtcctgg caggagatga attgggtggc gcagttacgt aggatttccc
    74341 agcccagcgg gttaggttcg ccacaggcca ggcctgccat gatggtggcc atatcaccgc
    74401 caacgttgac gatgtcgcct ttgacgccgg agcgataaat acagtcagct ttgtcaggtt
    74461 caacaataat gctgtgcaga ttttgcgggc tatagacgtc gaccagataa cccagcacac
    74521 caccggccat tgctccgaca ccggcttgca gcagaacgtg cgtcggggtt acgcccattt
    74581 cacgcatttg ctcgacggct tcatctgcca gggttgcgta gccttgcatg atccaggttg
    74641 ggattttggt gtaaccttcc catgccgtgt cctgtaccac ttcccagccg tgctgctgcg
    74701 cgtgttgcat ggtcaggcga acggtatcgt catagttcat atctgtgacg atgcactcgg
    74761 caccgaggtt cagaatggcg tcaacgcgtt cctgagcaga acctttcggc atgtaaatca
    74821 ccgcattctg tccgagttgc tgcgctgccc acgccacacc gcgcccgtgg ttgccgtcgg
    74881 tggtggtcgc gaaagtcatt ttttcgccga tggcattttt caggtgctca aatgacagcg
    74941 tttcgatatc aagatgatat ttttcacaca ataattgagc gatagcgtac gcaccgccga
    75001 gcattttgaa ggcgttcaga ccgaatcgtt ttgattcgtc tttgacaaga attttcttca
    75061 ccccaaaaag gtttgcgaga tcgtccagcg cacaaagcgg tgttgggcga taaccagcta
    75121 ttttctggtg gaactggcgc gccagtttgg cctggctttg cgaaaagagc ggtgatgttt
    75181 cgccgttgaa aaatttgtta tcggcgatat caatcttcaa tgagaaaacg gacatatcct
    75241 atccttttgg cctctggaaa tagagggccg accagctgat cggcccgagg ggttatttca
    75301 cgcgttcttg cgcttctttc agcagttgct cgagcagtgc acctggtttg gcgtatttac
    75361 gggacaggat catcgcggcg atgatataag gcttccagct ggcttctttg taggtagcga
    75421 tacggtattt ttcgaatacg ccttcagtca cttccccttc tttgcaggat acgccgctga
    75481 tatcagctgg caggcagtgc atgtacaggg cttcgccatc acgggtcagt tccatcatct
    75541 cttcagtaca atgccagtct ttgtgttgcg cgttctgtgc cagacactgt ttttccagtg
    75601 cttttaagcc ttcgtgatcg ttagcgcgca gcaattcggt acgctgttcc atcactttgt
    75661 aaggtgccca tgacttcgga taaacgatgt ctgcatcttt gaacgcttct tccatgctgg
    75721 tgacctgacg gaagctaccg ccagatgctt tagcattgtt tttcgccact tcaaccacat
    75781 ccgggatcag gtcgtagcct tccggatggg ccagggtgac atccataccg aagcgagtca
    75841 tcagaccgat gatgccttgt ggtacagaga gcggtttgcc atagcttgga gagtaggccc
    75901 aggtcatggc gattttttta cctttcaggt tttccagtga accaaagtgt tcacgtaacc
    75961 acgcgaggtc agccattgac tgagtcgggt ggtcaatatc gcattgcagg ttcactaaag
    76021 ccggacgctg tggcagtaca ccctgcttgt aaccgtcatc aagtgcagcg ccaacttcac
    76081 gcatataggc gttgcctgcg cccagataca tatcgtcgcg aataccaata gcgtcggcgc
    76141 agaaggagat catattggcg gtttcacgca cggtttcgcc gtgagcgatt tgtgatttgc
    76201 cttcgtcgag atcttgttgt gcaaggccga gcaggttaag cgcggaagca taagagaagc
    76261 gggtacgggt ggagttgtcg cggaatacgg aaatacctaa tccactatta aagactttgg
    76321 ttgagatgtt ttcagcacgc agtgctttta atgctgcggc aacgtccagt acttgtttca
    76381 gttcatctgg cgtctgttcc cacgttaaca aaaaatcttt ctcgtgaagg tgagaggtca
    76441 gcgaattgat atccttaatc agctcattaa cagttttcat cataaagtcc cggtagtgga
    76501 atgacaggca aagtggttta tttcactgtg acttgccaaa gtgattggtg ttttcacagc
    76561 tatacctggg actaacaata agtgtgccag aattatccac ggggctgcaa aagactaatt
    76621 tgacggataa tgtgtggggt tgatcacaaa ttgatgccag cttccggttg tttgtaaaat
    76681 acgaaaacgt ttggcttttt ttatcgcgtt atttatttgc tgctaagtat cagaaataag
    76741 ataaagggac aggctatctc tcttcagttt gataaactca ccgccgtaat tctcatattg
    76801 atatagaaat gtgatatctc ttccataatt tcattagaaa atgataaaaa tcttatgttt
    76861 ttatcaaagc caatttcgcc tgagttaata tcatcatgat ctgtgaacca tcaacgtctt
    76921 caatgatcga cttttaagta agtctggaag gtaaagctat ggagcttgct actacgcagt
    76981 cagtattgat gcaaattcaa ccgacaattc agcgttttgc cagaatgctt gccagcgttt
    77041 tgcagcttga ggttgagatc gttgatgaaa acttgtgtcg cgtcgccgga acgggcgcgt
    77101 ttgggaagtt tcttggtcgc cagttgagcg gcaactcacg cctgctccgc cacgtcctgg
    77161 aaacgaaaac tgaaaaagtt gtgacacagt ctcgcttcga tcccctttgc gaaggttgcg
    77221 atagtaaaga aaattgccgt gaaaaagcat ttctgggtac gcctgtcatt ttacaggatc
    77281 gttgtgttgg ggtgataagt ttgattgccg ttacccacga gcaacaagag catatcagtg
    77341 ataatttacg cgaattttct gattatgttc gccatatatc caccattttt gtttcgaaac
    77401 ttctggagga tcaggggcca ggagataaca tcagtaaaat attcgcgacc atgatcgata
    77461 atatggatca gggcgtatta gttgttgatg atgaaagtcg ggttcagttt gttaatcaga
    77521 ctgccttaaa aacacttggt gttgtacaaa ataatattat tgggaaacct atccgtttca
    77581 gaccattaac atttgagagt aattttactc atggacatat gcagcatatt gtttcgtggg
    77641 acgataaaag tgaattaatc attggtcaat tgcataacat tcagggccga caattatttt
    77701 taatggcatt tcaccaatcg cataccagtt tttctgtagc aaatgcacct gatgaaccac
    77761 atattgaaca attggttggc gagtgccgtg ttatgcggca attaaaacga ctcattagcc
    77821 gtattgcacc cagcccatcc agcgttatgg tggttggtga aagcggcacg ggtaaagaag
    77881 tcgtcgcccg agcaatccat aagttgagcg gaagacggaa taaacccttt attgctatca
    77941 actgtgccgc gattccggag cagcttctgg aaagcgaact gttcggttat gttaaaggcg
    78001 catttactgg cgcttctgcc aacggtaaaa cagggttgat tcaggcggcg aatacgggca
    78061 cgctgtttct cgatgaaata ggtgatatgc cattaatgtt gcaggctaaa ttactgcgcg
    78121 ctattgaggc gcgtgaaatt ctaccgattg gtgccagtag cccaatacaa gtcgacattc
    78181 gcatcatttc tgcaactaat cagaatttgg cccagttcat tgccgaaggt aaattccgcg
    78241 aagatctctt ctaccgactt aatgttatcc cgataactct gccaccgctg cgtgaacgtc
    78301 aggaagatat tgaactattg gtgcattact ttttacatct gcatacccgt cgtctgggat
    78361 cggtttatcc tggcattgct cccgatgtcg tcgaaatatt gcgtaagcat cgttggcccg
    78421 gaaacctgcg cgagttaagt aatttgatgg aatatctggt taacgtagtt ccttcaggtg
    78481 aagttatcga cagcacgcta ttgccgccaa atctgctgaa taatggcaca acggagcaaa
    78541 gtgatgtaac agaggtcagt gaggcgcacc tgtcactcga tgatgcgggc ggcacggcgc
    78601 tggaggagat ggaaaagcaa atgatccgcg aggcgctttc acgtcataac agcaagaagc
    78661 aagttgctga tgaactgggc atcggcattg ctacgctcta tcgcaagatt aagaaatatg
    78721 agttgttaaa cacataacct cagtgaatcc ggcctgaatt caggccggat atccttttac
    78781 ttcgttttct cgcaatccag aactgtattt acaatcatct gataccccgt gcagcgacaa
    78841 agatttcccg ccagtccgcg acgaatttcc gtaatggtta atggcttctc gcgtggtttc
    78901 gccagcattg ccgtggtagc cataatcagg ccaggcgtac aaaacccgca ctgcactgcg
    78961 ccggatttcg cataagcctg ctgaacatga gaaagttttc cgcctttcgc ttcaccttcc
    79021 agcgtgcgga tctcttttcc ttcagcccag gcggcaaggt ataagcaact gtctattgct
    79081 gtgccgtcga ccaacaccgt acaggcacca cattcaccca cgcagcaccc ttgtttgaca
    79141 cttagcagtc cttgttcgcg gagtaattcc gagagcggcg tgcctggtgc ggcgtgaagc
    79201 tgaaaaggca tcccgttaat ggtgcattcg atggtaattg tttcgctgtg attcattgca
    79261 attttccccc cgccgcggcg acggcttcgc taatcacttt tttggtcatc gtctggatga
    79321 gatgcagacg aaactcttta ctggcccgcc atgaagaacg cggggcgaca tcttgcagga
    79381 cagattcgct gatagcttcc agcgtttgca ggtttaatgg cgcattttgt gcagtctgtt
    79441 cggcatgttg gcagcgaatc ggcgttggcg cggcaacacc aaatgccagg cgtaattcgc
    79501 tgaaattgcc gttatccagt cggcaatgtg cggcgcagcc aatcgttgaa atatccattg
    79561 cgtcgcgcat ggcatattta aaatgcgcgc tgcccgcgtg ttctttcggc tgtggcggaa
    79621 aatgaaaggc gacgaggatt tcgtcatgct caagagacac tttgcccggc ccggtgtgaa
    79681 agccattaat cgggacgaaa cgaacaccgc gcggggagtg gatctccagt ttcgcgtcat
    79741 aaattagcgt tggcgtggca gaatctgcgc tggtggcacc gttgcaaata tttccaccgt
    79801 aggtagcgac gttacggatc tgcggtccag caatggacgt ggccgcagca cataacgccg
    79861 ggagatgacg ttgagttata ggatcttcta ttagctgggt aaatgtcgtt gcagagccga
    79921 tacgtagcga gccatcttcc gccagcgtaa ttccccgcag ctccgccaga ttatgaatat
    79981 caacaatatg gcgataacgg tcattgtgat ggtggagctg aatcagtacg tcagtgccac
    80041 cggcgagcag tttggcctgc gggttgtcag ccagcaggtt tatggcatcg gcaagggttg
    80101 ctgcgcgatg gtaagaagca aaatcaaaca tgatgttatc ctcaaatcaa tcctgccaga
    80161 tggaactctt catataaccg ttttggcgtc agcggcagtg tattgattgc aacaccggta
    80221 gccatcttca ccgcgttacg aatagcagcg gcaacaggaa ttattggtgg ctcacccagt
    80281 gacttatgtc cgtatgcgga ttgcggctca ttgatttcga cgaacgcgct ttccagttgt
    80341 ggcagatccg gcatggtcgg cattttgtaa tccagcagat tggggttacg gaccacgccg
    80401 cttttagcat cgatgatcat ctcttcaaat agcgcccagc caatgcccat tcccattccg
    80461 ccgtgtacct gaccttctgc cagcagtgga ttaagaatat gccctgaatc atgaacgttg
    80521 aggatgcggt tgatggtgac tttgcacagc gcaatatcga ccgtcagatc aacaaaggta
    80581 cagccaaacg ccggtgggtt agtggtggtt ttgatggagc tttcagcaga gagctgcccg
    80641 ccgcgttcag ggtggtagaa agcgtccatc gccaaatctt ttaacgacat taacggctct
    80701 tccggtcgtt caaccagcac gatatggcct tttatcaggg tcagattcat cgctgactga
    80761 tgtagcatga ctgcggcgtg agcgatgatt ttctctttta ataatagtgc cgcactgcgc
    80821 agcgcaggcg cggcaacata gctctggcgt gaggcaaatg cgccgggatc gaacggcgta
    80881 acgtcggtat cttgagttga aataacgcga acgtcgctga ccggaacccc cacggtttct
    80941 gccaccattt gcgagaagac ggtgtcggca ccctgaccga tttccgtcgc gccgctttgc
    81001 acgttgatgg ttccatcctg attcatcaga aggcgcgcgc ctgctatttc tacgccgaca
    81061 ggccaggtgt tagaggtgta gctaaaacag gcgacgccaa cgccgcggcg caaattgcct
    81121 tgctggttct ggcattctgc acggcgtttt tcccattcaa agattttccg gcctttttca
    81181 agacactccg gcaaccctgc gctgtaaata cgtttgcccg tgagcggatt agcatctcct
    81241 tcgcgggcgg cgttgcgtaa acgaatttca acaggatcaa tacctaacgc tgtcgcggcg
    81301 tcatcaagca tagactcaac ggcaaatacg acttgtggcg cgccataacc acgcatcgca
    81361 ccagccgagg ggaggttggt atagcaggtc tttgaactgt aagcgtaggc acaacgagga
    81421 taaaggtaag cgactttatt cccccccgca gaagcgatgg agtgcccgtg agatgcataa
    81481 gcgccggtgt tagacagaac atccagacta taacctttca atgttccgtc gcggttcacg
    81541 cccatttgcc cgtcaatggt aaaagcgtgg cgggtacggg ttgcgaggaa acactcttca
    81601 cggctaaggg aaactttcac cggaatgccg ccaagcttgc tggtcaggaa tgccgccatt
    81661 ggctcttcca gtacatcctg tttattacca aaaccgccac cgacaaacgg tttgatgact
    81721 cgtacgcatg accagggaat atccagcgcc tgaccaacca cgcggcgaac aatgtgcggg
    81781 atctgggtgc tggaaacgat ggtaattcgc gagtcatcct ccatccacgc cagcgatgtt
    81841 acgctttcca tgtgacaatg ttgaataacg ggggtctgat agtgcccctg tacctggtag
    81901 tcggcggcat cgattgtttg ttggacatta cccgtcgaca tcgtgctttg tttcagtaaa
    81961 ttgccaccgt tatggattgg tgcagcgtct tctgccagcg ccgcttctgg cgtggtgata
    82021 acgggtaatt cttgccactc aatgctgacc aattgcgccg ctttttctgc cgtgagttca
    82081 tcgcgggcca cgacgatggc aacggcgtca ccatgatgac gaacatggcg agttagcagt
    82141 gcgcgatcgg cggtatcgcg cttgttttcg tcaagtgtcc aggcatgccc agcagtagca
    82201 aatggaatat caggcacatc ttcccaggta aaaatcgcca gtacgcccgg caaacttctg
    82261 gcttgttcat cattaatact tacggcataa ccatgtgcga tagggctacg tacatatttc
    82321 gcataacaca tgcccgccat aacataatcg tcagtatatc gtgcccgccc ggtgacctta
    82381 gcaatggcat cgacgcgcat gcatgattca cccgtagcgg ttgcttcccg cgcttccata
    82441 gaaatcccct cgatttgaac atttggttct tatggcaaag atttagtcta agcaggcaag
    82501 atttacgcca gaaacgacga taagcgttag gggaggtagg aagtgtgatg acgttaaaaa
    82561 aacacagata tgatacgaaa caggactctg aataggggca gtgatacgta tcaatatgag
    82621 tggcgataaa atcaatgtga taaattggga ttattacgca tcaggatggg aaatagggta
    82681 aaaagtcagg aagttcgata cctctacagc gaatattttg ctgatgatac ttccctttgc
    82741 caaatgaaat taacctcttt ctctattacc acgtttttcc agaagcaaga gattgggtat
    82801 caagaggctg gctgctatga taaggcgtct tgttttttaa gcgaggaaag attttgagtg
    82861 cgggacgcct gaataaaaaa tctctgggta tcgtgatgtt gttatcggtt ggactgcttt
    82921 tggcgggctg ttcgggtagc aaatcatccg atacaggaac gtattccggc tccgtttaca
    82981 ccgtgaaacg gggggatacg ctatatcgta tttcgcgcac cacgggaacc agcgtaaaag
    83041 aactggcgcg actgaacggc atttcccccc cttacaccat tgaagttggt cagaaactaa
    83101 aactgggtgg ggcgaaaagt agcagtagta cacgtaaatc aaccgccaaa tcaacgacca
    83161 aaaccgcatc ggttacaccg tcatcagcgg taccgaaatc ttcctggccg ccagtagggc
    83221 aacgttgttg gttatggcca acgacaggga aagttatcat gccgtattcg acagcagatg
    83281 gcggcaataa agggattgat atctcagctc cacggggtac acctatttac gccgcgggtg
    83341 caggaaaggt ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc atcatgatta
    83401 aacacagtga agattacatt acggcttacg cccataatga cacgatgctg gtaaataatg
    83461 ggcaaagcgt gaaggctggg caaaaaatcg ccactatggg gagcacggat gcggcatctg
    83521 ttcgcctgca tttccagatt cgttaccgtg caacggcaat tgatccgcta cgttacttgc
    83581 cgccgcaggg cagcaagcca aaatgctgat ggcgaattaa tcagcagtca gcagcgcggc
    83641 acttgctaag gagagcgtaa ggtttataat gccttacgca tctcgaagcg ggcgtagttc
    83701 aatggtagaa cgagagcttc ccaagctcta tacgagggtt cgattccctt cgcccgctcc
    83761 aatttatctt cacctgatca atctgccatt tttgctgtat ctgattttct atattatctt
    83821 aattcaatcg cttcagactt ttcgcaatcg ccgtaatact tccccatttt gttgtaaata
    83881 aaaagcgttt acgcaatgca atccttttgt aacaacccag gtaaaacacc ttatt
//
LOCUS       DAZGTR010000008        73347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGTR010000008 DAZGTR010000000
VERSION     DAZGTR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.008"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            84..263
                     /locus_tag="ACHLCY_000618"
     CDS             84..263
                     /locus_tag="ACHLCY_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367936.1"
                     /translation="MQQLVPPPGVKGIDKMVHYEVVQYLMDCCGITYNQAVQALRSND
                     WDLWQAEVAIRSNKM"
     gene            274..429
                     /gene="ydaF"
                     /locus_tag="ACHLCY_000619"
     CDS             274..429
                     /gene="ydaF"
                     /locus_tag="ACHLCY_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaF family protein"
                     /protein_id="HFP0367937.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LEKHTVS"
     gene            complement(426..914)
                     /gene="sieB"
                     /locus_tag="ACHLCY_000620"
     CDS             complement(426..914)
                     /gene="sieB"
                     /locus_tag="ACHLCY_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection exclusion B family protein"
                     /protein_id="HFP0367938.1"
                     /translation="MLDVFTPLLKLFANEPLERLMYTIIIFGLTLWLIPKEFTVAFNA
                     YTEIPWLFQIIVFAFSFVVAISFSRLRAHIQKHYSLLPEQRVLLRLSEKEIAVFKDFL
                     KTGNLIITSPCRNPVMKKLERKGIIQHQSDSANCSYYLVTEKYSHFMKLFWNSRSRRF
                     NR"
     gene            1356..1577
                     /gene="kilR"
                     /locus_tag="ACHLCY_000621"
     CDS             1356..1577
                     /gene="kilR"
                     /locus_tag="ACHLCY_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFP0367939.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     NLTTKTCITDRMIKVFLGRDGLPVKAESW"
     gene            1577..1747
                     /gene="ydaE"
                     /locus_tag="ACHLCY_000622"
     CDS             1577..1747
                     /gene="ydaE"
                     /locus_tag="ACHLCY_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFP0367940.1"
                     /translation="MTKKIKCAYHLCKKDVEESKAIERMLHFMHGILSKDEPRKYCSE
                     ACAEKDQMAHEL"
     gene            1822..2097
                     /locus_tag="ACHLCY_000623"
     CDS             1822..2097
                     /locus_tag="ACHLCY_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367941.1"
                     /translation="MITNYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLDEGVKKMCLVHIGKNLPTEKKAEFLATLIAMKLKGEI"
     gene            2199..4799
                     /gene="recE"
                     /locus_tag="ACHLCY_000624"
     CDS             2199..4799
                     /gene="recE"
                     /locus_tag="ACHLCY_000624"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFP0367942.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSG
                     KKLSSYFKAVATNFPVVNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQG
                     NTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEEL
                     PAVTALAVSHKTSLLDPLEIRELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYT
                     DRGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARS
                     MDLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIG
                     IEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQP
                     SGTTAVEQGEAETMEPDATEHHQDTQPLDAQSQVNSVDAKYQELRAELHEARKNIPSK
                     NPVDADKLLAASRGEFVDGISDPNDPKWVKGIQTRDCVYQNQPETEKTSPDMNQPEPV
                     VQQEPEIACNACGQTGGDNCPDCGAVMGDATYQETFDEESQVEAKENDPEEMEGAEHP
                     HNENAGSDPHRDCSDETGEVADPVIVEDIEPGIYYGISNENYHAGPGVSKSQLDDIAD
                     TPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEEFSNRFIVAPEFNRRTNSGKEEE
                     KAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQWLVESAGHAESSIYWEDPETA
                     ILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHVQDAFYSDGYEAQFGVQPT
                     FVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLADCLNTDEWPAIKTLSL
                     PRWAKEYAND"
     gene            4792..5601
                     /gene="recT"
                     /locus_tag="ACHLCY_000625"
     CDS             4792..5601
                     /gene="recT"
                     /locus_tag="ACHLCY_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFP0367943.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAIKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRKVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFNFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            5657..5806
                     /gene="ralR"
                     /locus_tag="ACHLCY_000626"
     CDS             5657..5806
                     /gene="ralR"
                     /locus_tag="ACHLCY_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HFP0367944.1"
                     /translation="MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEK
                     EALER"
     gene            5844..6032
                     /locus_tag="ACHLCY_000627"
     CDS             5844..6032
                     /locus_tag="ACHLCY_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0367945.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            6132..6347
                     /gene="xisR"
                     /locus_tag="ACHLCY_000628"
     CDS             6132..6347
                     /gene="xisR"
                     /locus_tag="ACHLCY_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFP0367946.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            6349..7584
                     /gene="intR"
                     /locus_tag="ACHLCY_000629"
     CDS             6349..7584
                     /gene="intR"
                     /locus_tag="ACHLCY_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0367947.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEVTNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGVVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            7636..8571
                     /gene="ttcA"
                     /locus_tag="ACHLCY_000630"
     CDS             7636..8571
                     /gene="ttcA"
                     /locus_tag="ACHLCY_000630"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP0367948.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(8700..10073)
                     /gene="dbpA"
                     /locus_tag="ACHLCY_000631"
     CDS             complement(8700..10073)
                     /gene="dbpA"
                     /locus_tag="ACHLCY_000631"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP0367949.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(10103..10276)
                     /gene="ynaL"
                     /locus_tag="ACHLCY_000632"
     CDS             complement(10103..10276)
                     /gene="ynaL"
                     /locus_tag="ACHLCY_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP0367950.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(10551..11534)
                     /gene="zntB"
                     /locus_tag="ACHLCY_000633"
     CDS             complement(10551..11534)
                     /gene="zntB"
                     /locus_tag="ACHLCY_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP0367951.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(11548..11595)
                     /gene="ydaN"
                     /locus_tag="ACHLCY_000634"
     CDS             complement(11548..11595)
                     /gene="ydaN"
                     /locus_tag="ACHLCY_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP0367952.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            11789..13021
                     /gene="dgcM"
                     /locus_tag="ACHLCY_000635"
     CDS             11789..13021
                     /gene="dgcM"
                     /locus_tag="ACHLCY_000635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP0367953.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(13042..13605)
                     /gene="smrA"
                     /locus_tag="ACHLCY_000636"
     CDS             complement(13042..13605)
                     /gene="smrA"
                     /locus_tag="ACHLCY_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP0367954.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(13935..14843)
                     /gene="abgR"
                     /locus_tag="ACHLCY_000637"
     CDS             complement(13935..14843)
                     /gene="abgR"
                     /locus_tag="ACHLCY_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0367955.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTLLTASLITQFRRECGYLQS"
     gene            15019..16329
                     /gene="abgA"
                     /locus_tag="ACHLCY_000638"
     CDS             15019..16329
                     /gene="abgA"
                     /locus_tag="ACHLCY_000638"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP0367956.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            16329..17774
                     /gene="abgB"
                     /locus_tag="ACHLCY_000639"
     CDS             16329..17774
                     /gene="abgB"
                     /locus_tag="ACHLCY_000639"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP0367957.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTCNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            17811..19337
                     /gene="abgT"
                     /locus_tag="ACHLCY_000640"
     CDS             17811..19337
                     /gene="abgT"
                     /locus_tag="ACHLCY_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP0367958.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            19348..19863
                     /gene="ogt"
                     /locus_tag="ACHLCY_000641"
     CDS             19348..19863
                     /gene="ogt"
                     /locus_tag="ACHLCY_000641"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP0367959.1"
                     /translation="MLRLLEEKIATPLGPLWVMCDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            20058..20810
                     /gene="fnr"
                     /locus_tag="ACHLCY_000642"
     CDS             20058..20810
                     /gene="fnr"
                     /locus_tag="ACHLCY_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP0367960.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            20962..21912
                     /gene="uspE"
                     /locus_tag="ACHLCY_000643"
     CDS             20962..21912
                     /gene="uspE"
                     /locus_tag="ACHLCY_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP0367961.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHELAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(21962..22219)
                     /gene="ynaJ"
                     /locus_tag="ACHLCY_000644"
     CDS             complement(21962..22219)
                     /gene="ynaJ"
                     /locus_tag="ACHLCY_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP0367962.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            22463..23494
                     /gene="ynaI"
                     /locus_tag="ACHLCY_000645"
     CDS             22463..23494
                     /gene="ynaI"
                     /locus_tag="ACHLCY_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP0367963.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(23545..25158)
                     /gene="mppA"
                     /locus_tag="ACHLCY_000646"
     CDS             complement(23545..25158)
                     /gene="mppA"
                     /locus_tag="ACHLCY_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP0367964.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(25495..26394)
                     /gene="pgrR"
                     /locus_tag="ACHLCY_000647"
     CDS             complement(25495..26394)
                     /gene="pgrR"
                     /locus_tag="ACHLCY_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP0367965.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            26532..27452
                     /locus_tag="ACHLCY_000648"
     CDS             26532..27452
                     /locus_tag="ACHLCY_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0367966.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSIFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAIMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPTQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            27452..28384
                     /locus_tag="ACHLCY_000649"
     CDS             27452..28384
                     /locus_tag="ACHLCY_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP0367967.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQKALEKVIPFFNTHLG"
     gene            28402..29040
                     /locus_tag="ACHLCY_000650"
     CDS             28402..29040
                     /locus_tag="ACHLCY_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0367968.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
     gene            29349..30077
                     /gene="mpaA"
                     /locus_tag="ACHLCY_000651"
     CDS             29349..30077
                     /gene="mpaA"
                     /locus_tag="ACHLCY_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP0367969.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPATAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(30052..31017)
                     /gene="ycjG"
                     /locus_tag="ACHLCY_000652"
     CDS             complement(30052..31017)
                     /gene="ycjG"
                     /locus_tag="ACHLCY_000652"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP0367970.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVTTAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     IVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFVDLDGPTWLAVDVEPALQFTTGELHL"
     gene            31136..31642
                     /gene="tpx"
                     /locus_tag="ACHLCY_000653"
     CDS             31136..31642
                     /gene="tpx"
                     /locus_tag="ACHLCY_000653"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP0367971.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(31686..33227)
                     /gene="tyrR"
                     /locus_tag="ACHLCY_000654"
     CDS             complement(31686..33227)
                     /gene="tyrR"
                     /locus_tag="ACHLCY_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP0367972.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(33375..34436)
                     /gene="ycjF"
                     /locus_tag="ACHLCY_000655"
     CDS             complement(33375..34436)
                     /gene="ycjF"
                     /locus_tag="ACHLCY_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP0367973.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(34433..35830)
                     /gene="ycjX"
                     /locus_tag="ACHLCY_000656"
     CDS             complement(34433..35830)
                     /gene="ycjX"
                     /locus_tag="ACHLCY_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP0367974.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            35985..36983
                     /gene="ycjW"
                     /locus_tag="ACHLCY_000657"
     CDS             35985..36983
                     /gene="ycjW"
                     /locus_tag="ACHLCY_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0367975.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIVMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(37094..37999)
                     /gene="ompG"
                     /locus_tag="ACHLCY_000658"
     CDS             complement(37094..37999)
                     /gene="ompG"
                     /locus_tag="ACHLCY_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP0367976.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(38044..39126)
                     /locus_tag="ACHLCY_000659"
     CDS             complement(38044..39126)
                     /locus_tag="ACHLCY_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0367977.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGTSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(39140..39799)
                     /gene="pgmB"
                     /locus_tag="ACHLCY_000660"
     CDS             complement(39140..39799)
                     /gene="pgmB"
                     /locus_tag="ACHLCY_000660"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP0367978.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(39796..42063)
                     /gene="ycjT"
                     /locus_tag="ACHLCY_000661"
                     /pseudo
     CDS             complement(39796..42063)
                     /gene="ycjT"
                     /locus_tag="ACHLCY_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
     gene            complement(42060..43115)
                     /gene="ycjS"
                     /locus_tag="ACHLCY_000662"
     CDS             complement(42060..43115)
                     /gene="ycjS"
                     /locus_tag="ACHLCY_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0367979.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(43125..43913)
                     /gene="ycjR"
                     /locus_tag="ACHLCY_000663"
     CDS             complement(43125..43913)
                     /gene="ycjR"
                     /locus_tag="ACHLCY_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0367980.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(43931..44983)
                     /gene="ycjQ"
                     /locus_tag="ACHLCY_000664"
     CDS             complement(43931..44983)
                     /gene="ycjQ"
                     /locus_tag="ACHLCY_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP0367981.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(45014..45856)
                     /gene="ycjP"
                     /locus_tag="ACHLCY_000665"
     CDS             complement(45014..45856)
                     /gene="ycjP"
                     /locus_tag="ACHLCY_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0367982.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(45843..46724)
                     /gene="ycjO"
                     /locus_tag="ACHLCY_000666"
     CDS             complement(45843..46724)
                     /gene="ycjO"
                     /locus_tag="ACHLCY_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0367983.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(46745..48037)
                     /gene="ycjN"
                     /locus_tag="ACHLCY_000667"
     CDS             complement(46745..48037)
                     /gene="ycjN"
                     /locus_tag="ACHLCY_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0367984.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(48051..49730)
                     /gene="ycjM"
                     /locus_tag="ACHLCY_000668"
                     /pseudo
     CDS             complement(48051..49730)
                     /gene="ycjM"
                     /locus_tag="ACHLCY_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(49942..50256)
                     /gene="pspE"
                     /locus_tag="ACHLCY_000669"
     CDS             complement(49942..50256)
                     /gene="pspE"
                     /locus_tag="ACHLCY_000669"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP0367985.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(50331..50552)
                     /gene="pspD"
                     /locus_tag="ACHLCY_000670"
     CDS             complement(50331..50552)
                     /gene="pspD"
                     /locus_tag="ACHLCY_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP0367986.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(50561..50920)
                     /gene="pspC"
                     /locus_tag="ACHLCY_000671"
     CDS             complement(50561..50920)
                     /gene="pspC"
                     /locus_tag="ACHLCY_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP0367987.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(50920..51144)
                     /gene="pspB"
                     /locus_tag="ACHLCY_000672"
     CDS             complement(50920..51144)
                     /gene="pspB"
                     /locus_tag="ACHLCY_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP0367988.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(51198..51866)
                     /gene="pspA"
                     /locus_tag="ACHLCY_000673"
     CDS             complement(51198..51866)
                     /gene="pspA"
                     /locus_tag="ACHLCY_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP0367989.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            52033..53010
                     /gene="pspF"
                     /locus_tag="ACHLCY_000674"
     CDS             52033..53010
                     /gene="pspF"
                     /locus_tag="ACHLCY_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP0367990.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(53130..54395)
                     /gene="puuE"
                     /locus_tag="ACHLCY_000675"
     CDS             complement(53130..54395)
                     /gene="puuE"
                     /locus_tag="ACHLCY_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP0367991.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDTAMKILQDALSD"
     gene            complement(54433..55713)
                     /gene="puuB"
                     /locus_tag="ACHLCY_000676"
     CDS             complement(54433..55713)
                     /gene="puuB"
                     /locus_tag="ACHLCY_000676"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP0367992.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTDNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(55715..57202)
                     /gene="puuC"
                     /locus_tag="ACHLCY_000677"
     CDS             complement(55715..57202)
                     /gene="puuC"
                     /locus_tag="ACHLCY_000677"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP0367993.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLTDLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(57477..58034)
                     /gene="puuR"
                     /locus_tag="ACHLCY_000678"
     CDS             complement(57477..58034)
                     /gene="puuR"
                     /locus_tag="ACHLCY_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP0367994.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(58061..58825)
                     /gene="puuD"
                     /locus_tag="ACHLCY_000679"
     CDS             complement(58061..58825)
                     /gene="puuD"
                     /locus_tag="ACHLCY_000679"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP0367995.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            59037..60455
                     /gene="puuA"
                     /locus_tag="ACHLCY_000680"
     CDS             59037..60455
                     /gene="puuA"
                     /locus_tag="ACHLCY_000680"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP0367996.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            60604..60768
                     /locus_tag="ACHLCY_000681"
     CDS             60604..60768
                     /locus_tag="ACHLCY_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0367997.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            60758..62143
                     /gene="puuP"
                     /locus_tag="ACHLCY_000682"
     CDS             60758..62143
                     /gene="puuP"
                     /locus_tag="ACHLCY_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP0367998.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALFVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            62277..62522
                     /gene="ymjA"
                     /locus_tag="ACHLCY_000683"
     CDS             62277..62522
                     /gene="ymjA"
                     /locus_tag="ACHLCY_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFP0367999.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            62835..64478
                     /gene="sapA"
                     /locus_tag="ACHLCY_000684"
     CDS             62835..64478
                     /gene="sapA"
                     /locus_tag="ACHLCY_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP0368000.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYRKKQDEVKKP"
     gene            64475..65440
                     /gene="sapB"
                     /locus_tag="ACHLCY_000685"
     CDS             64475..65440
                     /gene="sapB"
                     /locus_tag="ACHLCY_000685"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP0368001.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            65427..66317
                     /gene="sapC"
                     /locus_tag="ACHLCY_000686"
     CDS             65427..66317
                     /gene="sapC"
                     /locus_tag="ACHLCY_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP0368002.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            66317..67309
                     /gene="sapD"
                     /locus_tag="ACHLCY_000687"
     CDS             66317..67309
                     /gene="sapD"
                     /locus_tag="ACHLCY_000687"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP0368003.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            67311..68117
                     /gene="sapF"
                     /locus_tag="ACHLCY_000688"
     CDS             67311..68117
                     /gene="sapF"
                     /locus_tag="ACHLCY_000688"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP0368004.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            68185..68538
                     /gene="ycjD"
                     /locus_tag="ACHLCY_000689"
     CDS             68185..68538
                     /gene="ycjD"
                     /locus_tag="ACHLCY_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFP0368005.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            68906..69694
                     /gene="fabI"
                     /locus_tag="ACHLCY_000690"
     CDS             68906..69694
                     /gene="fabI"
                     /locus_tag="ACHLCY_000690"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP0368006.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            69839..70966
                     /gene="yciW"
                     /locus_tag="ACHLCY_000691"
     CDS             69839..70966
                     /gene="yciW"
                     /locus_tag="ACHLCY_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP0368007.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            71034..72968
                     /gene="rnb"
                     /locus_tag="ACHLCY_000692"
     CDS             71034..72968
                     /gene="rnb"
                     /locus_tag="ACHLCY_000692"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP0368008.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(73046..73150)
                     /locus_tag="ACHLCY_000693"
     CDS             complement(73046..73150)
                     /locus_tag="ACHLCY_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368009.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            73204..>73347
                     /locus_tag="ACHLCY_000694"
     CDS             73204..>73347
                     /locus_tag="ACHLCY_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368010.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSA"
BASE COUNT        18273 a        18793 c        18157 g        18124 t
ORIGIN      
        1 tacttcgagt taccaggcag tggtcagggg ttaagtagcc agcccgaggc gtatgaacat
       61 gacggcggga ttcaaatttt gcagtgcagc agttagttcc gccacccggc gttaagggga
      121 tagataagat ggtgcattac gaagtagttc agtatttgat ggattgttgc ggtatcactt
      181 acaaccaggc tgtgcaggct ttacgcagca acgactggga tctctggcag gcagaagtcg
      241 ctatacgtag caacaagatg tgagattcgc aaaatgcaaa aaatcgacct cggcaacaac
      301 gaatccctgg tgtgcggcgt gttccccaac caggatggaa cgttcactgc catgacgtat
      361 accaaaagca aaacatttaa aaccgaaact ggtgcgcgcc gatggttgga gaagcacaca
      421 gtaagctaac gattaaaacg tctactcctg ctgttccaga ataacttcat aaaatgggag
      481 tatttttcgg tgacgagata ataagaacag tttgcgctat cactctgatg ttgaatgatg
      541 cccttccgtt ctaatttttt cataaccggg ttacggcaag gagaagtgat aataagattt
      601 cctgttttaa ggaaatcttt aaatacagcg atttctttct cagataaacg aagcaatact
      661 cgttgctctg gtagtaatga ataatgcttt tgaatatgtg ctcgcaatct tgagaaggaa
      721 atggcgacca cgaaagaaaa ggcaaaaacg ataatctgaa agagccaagg tatttcagta
      781 taagcattga atgcgacagt aaactctttc ggtatcagcc agagagtgag accaaaaatg
      841 ataatcgtat acataagtct ttcgagtggc tcgttagcaa aaagtttcaa caatggagta
      901 aatacatcca acatatcaat aactctcaac tgtaagggta ttgaaatgtt aacacaagct
      961 ctcgctgtag gggtatagcc gagaccaccg aagcccggag gtggtgaaat aaaaccgggc
     1021 acaacacgaa ggcgcatttc cgatatccat aaagagtcgg tcttgtctgt taaatttaaa
     1081 tggtgggagt gcgcctccgg ttgtaaataa cgacattgct gtgtgtagtc ctggcggcat
     1141 cagttttttt cttgaagttc ggctgatgtc cgcccttttt aaagtgaatt ttgtgatgcg
     1201 gtgaatgcgg ctaagcgcac gtggcacagt taaaagtcat gttagtcctt attggtttgg
     1261 gtgggaaagc cgactgtaat tgttaactgg ttgcagtcac ctggaggcac caggcaccgc
     1321 atcaacaaag ttcatttgta aaaatggaga taattatgat tgcacatcac ttcggaactg
     1381 atgaaatacc acgtcagtgt gtgactcctg gcgattatgt tcttcatgaa ggccggacat
     1441 atattgcctc ggcaaacaat attaaaaagc gaaaactata tattcgtaac ctgaccacaa
     1501 aaacatgcat tactgaccgc atgattaaag tcttcctcgg tcgtgatggt ttacctgtaa
     1561 aggcggagtc atggtgatga ctaagaaaat aaaatgtgct taccaccttt gcaaaaaaga
     1621 cgttgaagaa agcaaagcta ttgaaagaat gcttcacttc atgcacggga ttttatcaaa
     1681 agacgaaccg agaaaatatt gcagtgaagc ttgtgccgaa aaagaccaga tggcacatga
     1741 actttaatta attgactatt cgaaactgaa tttatgccag aaatggcagg tattcgctca
     1801 accttaatta aggagaaaaa catgattacc aattatgaag ccactgttgt aactaccgat
     1861 gacattgttc acgaggtgaa tctggaagga aagcgcattg gctacgtaat taaaacagaa
     1921 aataaagaaa ccccattcac tgtggttgat atcgatggtc catcaggcaa cgtaaaaaca
     1981 cttgatgaag gtgtcaaaaa aatgtgcctg gtgcatatcg gaaagaatct gcccacagaa
     2041 aaaaaagccg aatttctggc aactctaatt gcaatgaaat taaaaggtga aatctgaaag
     2101 aaatagcctg cgtatggcgc aggctatgaa cagtgtgtat ccggcaagat cattcactga
     2161 acaaaacgaa ttttaatctg agttgaggtt aaaaaacaat gagcacaaaa ccactcttcc
     2221 tgttacggaa agcgaaaaaa tcatccggtg aacctgacgt cgtcctgtgg gcaagcaacg
     2281 attttgaatc gacctgtgcc actctggact acctgatcgt taagtcaggt aaaaaactga
     2341 gcagctattt taaagctgtt gccacgaatt ttcctgtcgt taatgacctg cccgctgaag
     2401 gtgagatcga ttttacctgg agtgaacgct atcaactcag caaagactcc atgacatggg
     2461 aactaaaacc gggagcagca ccagacaacg ctcactatca aggcaatacc aacgtcaacg
     2521 gcgaagacat gactgagatt gaggagaata tgctactccc aatttctggc caggaactgc
     2581 ccattcgttg gcttgctcaa cacggcagcg aaaaaccggt aacgcacgtt tcacgcgacg
     2641 gactccaggc attacacatt gctcgggctg aagaactacc ggctgttact gccctggctg
     2701 tttcccacaa aaccagcctg ctcgacccgc tggaaattcg cgaactccac aaactggttc
     2761 gtgacactga caaagttttc cctaatcctg gtaattcaaa cctgggactg ataactgctt
     2821 ttttcgaagc atacctgaac gctgactaca ccgatcgagg actgctgaca aaagagtgga
     2881 tgaagggtaa tcgtgtttca cacatcactc gcacggcttc cggtgctaat gctggcggcg
     2941 gaaacctcac cgatcgcggc gaaggtttcg tacacgatct gacgtcactg gcgcgcgacg
     3001 tagccactgg cgtactggcc cgttcaatgg atctggacat ctataacctt catccggcac
     3061 acgctaaacg cattgaggaa attatcgctg aaaataaacc gcccttttct gttttccgcg
     3121 acaaattcat caccatgcct ggcgggctgg attattcccg cgccatcgtg gttgcgtccg
     3181 taaaagaagc accaattggg atcgaggtca tccccgcgca cgtcactgaa tatctgaaca
     3241 aagtactgac tgaaaccgat catgccaacc ctgatccgga aatcgtggat attgcctgcg
     3301 gtcgctcctc tgccccgatg ccgcagcgag taacagaaga aggaaaacag gatgatgaag
     3361 aaaaaccgca accatctgga acaacggcag ttgaacaggg agaggctgaa acaatggaac
     3421 cggacgcaac tgaacatcat caggacacgc agccgctgga tgctcagtca caggtaaatt
     3481 ctgttgatgc gaaatatcag gaactgcggg cagaactcca tgaagcccgg aaaaacattc
     3541 catcaaaaaa tcctgtcgat gccgataaat tgcttgctgc atcacgtggt gaatttgttg
     3601 acggaattag cgacccgaac gatccgaaat gggtaaaggg gatccagact cgcgattgtg
     3661 tgtaccagaa ccagccagaa acggaaaaaa ccagcccgga tatgaatcaa cctgagccag
     3721 tagtgcaaca ggaaccggaa atagcctgca atgcctgcgg ccagactggc ggggataact
     3781 gccctgactg tggtgcggtg atgggcgacg caacatacca ggaaacattc gatgaagaga
     3841 gtcaggttga agctaaggaa aatgatccgg aggaaatgga aggcgctgaa catccgcaca
     3901 atgagaatgc tggcagcgat ccgcatcgcg attgcagtga tgaaactggc gaagtcgcag
     3961 atcccgtaat cgtagaagac atagagccag gtatttatta cggaatttcg aatgagaatt
     4021 accacgcggg tcccggtgtc agtaagtctc agctcgatga cattgctgat actccggcac
     4081 tgtatttgtg gcgtaaaaat gcccccgtgg acaccacaaa gacaaaaacg ctcgatttag
     4141 gaaccgcttt ccactgccgg gtacttgagc cggaagaatt cagtaaccgc tttatcgtag
     4201 cacctgaatt taaccgccgg acaaactccg gaaaagaaga agagaaagcg tttctgaggg
     4261 aatgcgcaag cacaggaaaa acggttatca ctgccgaaga aggccggaaa attgaactca
     4321 tgtatcagag cgttatggct ttgccgctgg ggcaatggct tgttgaaagc gccggacacg
     4381 ctgaatcatc aatttactgg gaagatcctg aaacagcaat tttgtgtcgg tgccgtccgg
     4441 acaaaattat ccctgaattt cactggatca tggacgtgaa aactacggcg gatattcaac
     4501 gattcaaaac cgcttattac gactaccgct atcacgttca ggatgcattc tacagtgacg
     4561 gttatgaagc acagtttgga gtgcagccaa ctttcgtttt tctggttgcc agcacaacta
     4621 ttgaatgcgg acgttatccg gttgaaattt tcatgatggg cgaagaagca aaactggcag
     4681 gtcagctgga atatcaccgc aatctgcgaa ccctggctga ctgcctcaat accgatgaat
     4741 ggccagctat taagacgtta tcactgcccc gctgggctaa ggaatatgca aatgactaag
     4801 caaccaccaa tcgcaaaagc cgatctgcaa aaaactcagg gaaaccgtgc accagcagca
     4861 attaaaaata acgacgtgat tagttttatt aaccagccat caatgaaaga gcaactggca
     4921 gcagctcttc cacgccatat gacggctgaa cgtatgatcc gtatcgccac cacagaaatt
     4981 cgtaaagttc cggcgttagg aaactgtgac actatgagtt ttgtcagtgc aatcgtacag
     5041 tgttcacagc tcggacttga gcccggtagc gccctcggtc atgcatattt actgcctttt
     5101 ggtaataaaa acgaaaagag cggtaaaaaa aacgttcagc taatcattgg ctatcgcggc
     5161 atgattgatc tggctcgccg ttcaggtcaa atcgccagct tgtcagcccg tgttgtccgt
     5221 gaaggtgacg agtttaattt cgaatttggc cttgatgaaa agttaataca ccgcccagga
     5281 gaaaacgaag atgcccctgt tacccacgtc tatgctgtcg caagactgaa agacggaggt
     5341 actcagtttg aagttatgac gcgcaaacag attgagctgg tgcgcagcca gagtaaagct
     5401 ggtaataacg ggccgtgggt aactcactgg gaagaaatgg caaagaaaac ggctattcgt
     5461 cgcctgttca aatatctgcc cgtatcaatt gagatccagc gtgcagtatc aatggatgaa
     5521 aaggaaccac tgacaatcga tcctgcagat tcctctgtat taaccgggga atacagtgta
     5581 atcgataatt cagaggaata attcagcctg gcggtgtaat gcaccgccaa cttgaaatat
     5641 ttttatgaga aaaattatga gatatgacaa tgttaaacca tgtccatttt gtggttgtcc
     5701 atcagtaacg gtgaaagcca tttcaggata ttaccgagcg aagtgtaacg gatgcgaatc
     5761 ccgaaccggt tatggtggaa gtgaaaaaga agcactcgaa cgatgaaata aacgaaccac
     5821 tggaaataat aatggaggtg ttcatgtata aaattaccgc cactattgaa aaggaaggtg
     5881 gcactcctac taactggaca agatattcaa aatctaaact aacgaaatca gaatgcgaaa
     5941 aaatgctctc aggtaaaaaa gaagcaggcg tttccagaga gcagaaagta aaactgataa
     6001 attttaattg cgagaaactt cagtcctcgt gaattgcatt gtattcaaat taaaacttca
     6061 tagctgatta ttaataatca acatcgggcg tcaatttaag tctaacattg gcgcctgcca
     6121 gaggtgatgc gatggcacaa gtaatcttta atgaagagtg gatggttgaa tacggcctga
     6181 tgcttcgcac tggtctgggg gccagacaaa ttgaagcata ccgccagaac tgttgggtgg
     6241 aaggcttcca cttcaaacga gtatctcctt tagggaagcc agacagtaag cgagggatta
     6301 tctggtataa ctatccaaag ataaatcagt ttatcaaaga ctcatgatat gtctaaatta
     6361 ccaacaggtg tcgagattcg aggtaaatac attcgcatct ggttcatgtt tcgaggaaaa
     6421 cgatgtcggg aaacattgaa aggctgggag gttactaaca gtaacattaa aaaagccggg
     6481 aatttaagag cgttgatagt tcatgaaatc aattccggtg agtttgagta tttaagacgt
     6541 tttccccagt ccagcactgg ggcaaaaatg gtgacaacga gggtcataaa aacgttcggg
     6601 gagctttgtg atatctggac aaaaattaaa gaaacagagt taacaacaaa cacaatgaag
     6661 aaaacgaaat cacaattaaa aacactcagg ataataattt gtgagagtac tccgatatcg
     6721 catattcgtt atagcgatat cttaaactac cggaatgaac tgctgcatgg agaaacgctt
     6781 tacctggata atccaagatc caacaaaaaa ggaagaaccg tgcgcacagt tgataactat
     6841 atcgccctgc tctgttcgtt gttacgtttt gcgtatcagt cgggatttat atcaaccaaa
     6901 ccatttgaag gagtaaagaa attacagaga aacagaataa agcctgaccc gttatctaaa
     6961 acagaattca atgcattaat ggaaagtgaa aaaggacaga gccagaactt gtggaaattt
     7021 gccgtatact ccgggcttcg tcacggggaa ctggctgctc tggcgtggga ggatgtggat
     7081 ttcgagaagg gagttgtgaa tgtcagaaga aacctgacga tactggatat gttcggtccc
     7141 ccaaaaacaa atgcggggat ccggacggta acactactgc agcctgctct tgaagcactg
     7201 aaggagcaat acaaactgac cgggcatcat cgcaaaagcg aaatcacctt ttatcatcgg
     7261 gagtacggca gaaccgaaaa gcaaaaactg cattttgttt tcatgcccag ggtgtgtaac
     7321 ggaaaacaaa aaccttatta ctcggtaagc agtttggggg caaggtggaa tgcagcagta
     7381 aaacgtgctg gtattcgccg ccgtaatccg taccatacgc ggcatacttt tgcctgctgg
     7441 ctgttgacgg caggagcgaa cccggcattt atagccagcc aaatggggca tgaaactgcg
     7501 cagatggtgt atgaaattta cggtatgtgg attgatgaca tgaacgacga acaggtagcc
     7561 atgttgaatg cgcggttatc atagttgcaa agtttgcccc caatttgccc catttagtac
     7621 cagagaactg aaataatgca agaaaatcaa caaattacaa agaaagaaca atacaacctg
     7681 aacaaattac aaaaacgtct gcgtcgtaac gtgggcgaag ccattgctga cttcaatatg
     7741 attgaagaag gcgatcgcat catggtttgc ctctccgggg gtaaagacag ctataccatg
     7801 ctggagattc tgcgcaattt gcagcaaagc gcgccaatca atttttcgct ggtggctgtt
     7861 aacctcgatc aaaagcaacc gggcttcccg gaacacgttc tgcccgagta tcttgaaaag
     7921 ctgggcgttg agtacaagat tgttgaagag aatacttacg gtatcgtgaa agagaagatt
     7981 ccagagggca aaaccacttg ctcactgtgt tctcgccttc gtcgcggtat cctttatcgt
     8041 accgcaacgg aactgggggc gacgaagatc gcgttgggtc accatcgtga cgatatcctg
     8101 caaacgttgt tcttaaatat gttctacggc ggcaagatga aaggtatgcc tccgaaactg
     8161 atgagcgatg atggcaaaca tatcgttatt cgtccgctgg cctactgccg cgagaaagat
     8221 attcagcgat ttgccgatgc aaaagcgttc ccgattattc cgtgcaacct gtgcggttca
     8281 cagcctaacc tgcaacgtca ggtgattgct gacatgttgc gtgactggga taaacgttat
     8341 ccagggcgta tcgagacgat gttcagcgcg atgcagaatg tggtgccgtc gcatctgtgc
     8401 gataccaacc tgttcgattt taaaggcatt acccacggtt ctgaagtggt taacgggggt
     8461 gatctggcgt ttgatcgcga agagatccca ctacaaccgg cgggctggca gccagaagaa
     8521 gatgaaaatc agttggatga gttacgactg aatgtggttg aagtgaaata accaggatag
     8581 cgcccgatgc gcaagcgtat cgggctactc ttatggaggc cggataagac gcggccagcg
     8641 tcgcatccgg caatcccgaa taagatgttt actcttgcac ccggcaattc aacatttcat
     8701 tattttaata accgcacccg gcacgttttt cctttaatct tcccgccctg taactgtttc
     8761 catgctttat gagcaacagc ctgacggacc gcgacataga catgcgccgg atgcacggcg
     8821 attttgccaa tatctgcgcc atcaagcccg atatctcctg tcagtgcacc taatacatca
     8881 cccgggcgca ttttggcttt tttcccgcca tcgatacaca acgttgccat ttctgcttcc
     8941 agcggcacaa tggaactatt agctggcggc gtttgccagt taagttttat ctgcaacatg
     9001 tcagaaatga tattggcccg ctgtgcttct tccggagcac agaaactgat cgccagaccg
     9061 ctatttcctg cacgagctgt acgaccgatg cgatgtacat gaacttcagg gtcccacgcc
     9121 agctcaaagt tcaccaccag ctcaagcgat ttaatatcca gaccacgcgc agcaacatca
     9181 gtagcaacca gtacacgggc gctaccgtta gcaaaacgta ccagagtctg atcgcgatcg
     9241 cgttgctcca gatcgccgtg taacgacaat gcactttgcc ctacttcatt cagcgcgtcg
     9301 cagacagcct ggcaatcttt tttggtattg caaaacacca cgcaagagga tggctgatgc
     9361 aagcttaata accgttgcaa cagaggaatt ttgcctttgc tggatgtctc ataaaattgt
     9421 tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg atcgcgttgt
     9481 actcgtccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag aagcgtctgt
     9541 cgagatgcag gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc catatccagc
     9601 atgcggtcgg cctcatccat caccaaagta ttcagcgcat ccagtgatac cgtgcctttt
     9661 tgcaagtgat ccagcaaacg acccggcgtt gccacgataa tatgcggcgc atgttgcaac
     9721 gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt ggtatttggc
     9781 agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc acgcgtagga
     9841 cacagcacta aagcctgggt ttgaaatagc gacgcatcaa tttgctgtaa caagccgagg
     9901 ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc ttttccggca
     9961 aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc caactcatta
    10021 aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt cacaatctat
    10081 tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct cgcagatcta cgctcacgat
    10141 gcgacaattt aatcggttct tcatcgggtg gtgggtcagg catgggttgc gggcgaggga
    10201 tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga atttgcaaat
    10261 aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg gtagacgctg
    10321 ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga atcaattgtg
    10381 ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc atcgcttgac
    10441 gttgcattca cctgcaatgg agatattgcc ctgaatgggt agatagttta ttgacttcgc
    10501 tcaaactttg cggcgttttt gtatacagac agccggaaaa attgcttttg ttacaaccat
    10561 ttactacggt gcaaccataa agcaacacca ccaataagaa caactaacag aatacaaaaa
    10621 attgaaaatc cgaattgcca cccgccgcca gggatcccac caaggttgac gccaaataac
    10681 cctgtcagaa aggtactggg taaaaagacc attgccatca atgacattgt ataggtacga
    10741 cgagctaaat tttcctgcat cacctgagcg atttcatccg ccatcacacc agtccgtgct
    10801 atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc ctgcatccga
    10861 cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc atagacatca
    10921 cgttgcggtg ccatatagcg acgcatcacg attaattgtt tgcgcagcag agccaggaat
    10981 ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat tttatcgtgc
    11041 agctgctcga taaattcgct ggaatgatcg gtcaacgcat cgcacacatc caccagccac
    11101 cctccgcaat cggtcggacc cgtgccctct tccagatcgc tcaccacatc gtccagcgcc
    11161 agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac acgcatggcg
    11221 accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaatgt aataagcgtg
    11281 ccttcaccga gacggctgac tcggggccgt gtgctctcgc ccgccagcgc atcacgtacg
    11341 ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg tacataatta
    11401 aggtggagcc agcagggatg cgcttcatca atcacatctg tattttccag cggtttaacg
    11461 ccgcctctac catccagcat ccaggcaaat actgcatccg ggacattaac gtccgatccc
    11521 ttaatcgctt ccacagtgcc tccatcatca acgcattatt ttgtagtcta gccttctggc
    11581 cctgttacgc aacatctcat caccccatta ccctgaaatg attaataaaa ttctgtctaa
    11641 attgaataca aaaagcaaaa tgcttttccg tatacaaacc gtgtgaagtg ttaaatagcg
    11701 tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc ggatagcaac
    11761 aaaaattgat aaaaataacg ggatctcaat gattacgcac aacttcaata ccctggactt
    11821 actcaccagt cctgtctgga ttgtttcgcc ctttgaggaa cagttaattt atgccaatag
    11881 cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg gaccctattc
    11941 cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc aacacgatat
    12001 tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca gcattaagct gtcggcttgt
    12061 tttgcgagag ctgacagaaa cagaaccggt gattattttc gaaggtatcg aagcgccggc
    12121 aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac aaggttttta
    12181 tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccct cacgagatgg
    12241 ccaaatcgtc gatgctaacc tcgccgcgct caatttctat ggttataacc atgaaacgat
    12301 gtgccagaaa catacctggg aaattaatat gctcgggcgt cgcgtcatgc ctatcatgca
    12361 tgaaatttcg catttacccg gcggtcataa acctttgaat tttgttcata aactggcgga
    12421 tggatctact cgtcatgtgc agacctatgc cgggccgatt gaaatttatg gcgacaagct
    12481 catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc agctggaaca
    12541 tgctgctcac catgacgcga tgaccggatt actgaatcgg cgacagtttt atcacattac
    12601 cgagccaggc caaatgcagc acctcgccat cgcccaggat tacagcttgt tgctcatcga
    12661 caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg atgaggtgtt
    12721 atgcgccctc gcccgcaccc tcgaaagttg cgctcgcaaa ggcgatttgg tgtttcgttg
    12781 gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg cgctttcgct
    12841 ggctgaaact atccgtgtaa gcgtggcaaa agtgagtatt tcgggcttac cacgttttac
    12901 cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac tgtttaaacg
    12961 cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc tggcggcata
    13021 agccgcggat gcgtctcgag atcaacggct gcgcttagcg tggcgctccc agttttcttg
    13081 cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc catgatgcgg
    13141 tagcgcggtg caatatgcct gaacatcatc aaattcggtc agccagcgcg ccacatagct
    13201 gcggacaata ttggcatgcg atttatcatc ccgtcctttg ccatgaataa tcagcacgtt
    13261 acgcaaacca tcagccatgg cttgttgaat gaaactgaac atcattttgc ggcactcttc
    13321 caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac tgcgcagctt
    13381 atccagcacc ccatgttgca acccttcccg ccgaaactcc agcggctgac ttagtgggat
    13441 gatgtcgaga aatccggtgg tgaggaaatt atcaagctgc agcgtgtcga tacgctgcgg
    13501 cgcacgttgg ttacgcgttg gatgccagtg gacatcggta gcacgtttca gcggctggac
    13561 atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgttac atcctcccgc
    13621 aattaagagc gcgatatgat aaccagaccg ggtcggtcca acaacgtctt acccaaaatt
    13681 ccagtaataa gttccaaata ttgccgatat tttaagcaaa atgcttatgc atgattattc
    13741 attcacgata ttaataatgt aacttatatt ttcgtgaaat ctgtcactga agaaaattgg
    13801 caactaaagg ttaaaaccgt tataacacag tcaccggcgc agaggagaca atgccggact
    13861 taagacgcgg atgcactgct gtgtgtactg tagagtctgg cggatgtcga cagactctat
    13921 ttttttatgc agttttaact ttgcagatag ccgcattctc gccggaattg cgtgattaat
    13981 gacgcggtca gtagtgtctg gcgactatca cgccgctgaa tcaaataata ggccgctttc
    14041 ggtaaaattt cgctaaccgg caacatcacc agcccctgtc cgtgcaaggg atcgcagccc
    14101 atttcttcag gcagtatgct gagaaaatcg ctttttgcca ccagactgat acaggctgag
    14161 aacgtctcgc agactacacc gacctgtggc gtttgcgcct gatcgtcaag caattcactc
    14221 aactgtttgt agtaactgcc gtgtggcgtc ggcattgtcc agctgtaatc cagtaactgt
    14281 ttgatcgaac gggcaccaat ggcggggtgt cccgggcggc aaaagatcgc gaattgcttt
    14341 tccagtaatt tctcaaaagt aaattcgtgg tcgtacggtc cctgataata ggtattgatg
    14401 gtgaaatcca attctccctg acgcaattca ttaatcatcg acaccagttg cccttccata
    14461 atgcgtactt ttacctgcgg atgctgctga tgaaaacgag atatgacagc tggcatcaga
    14521 ctgcgggaaa tactggcccc catgccgata ttaatctgcc ctgccagttg cccttgtcgt
    14581 tggcgaattt cctcttgcgc tgcgcgcagc tcttcaagaa taagactggc gtgctgataa
    14641 aaactttcac cggcatcagt taacgtcacg cctttactac ggcgaaaaaa gagttgcgcc
    14701 gctaatcctt cttccagctc ctgaatagat ttactcagtg ccggttgcga catattcaac
    14761 attcggctcg ctccgcgaat gctgccctga cgagccactt caacaaaagc ccgaatttga
    14821 tgaattttta cctgaaaagc catgacgcca ccgataaccg ttatttatca gaccaaagaa
    14881 actggcatct actttaatgc agatgattgt gtcagggtaa tttatgaacg gttaaaactg
    14941 tgaaaaatca gttagtgata agtaaaaact atcgctacgt gaaccgggtc acacttttta
    15001 ctgatgacgg gaaaggttat ggagtcgttg aatcaatttg ttaattcgct tgccccaaaa
    15061 ttatcgcact ggcgacgtga ttttcatcac tatgcagagt ctggctgggt ggaattccgc
    15121 actgccaccc ttgttgcgga agaattgcag cagctcggct attcactggc gctgggccgc
    15181 gaagtagtta atgaaagtag ccggatggga ctacctgatg aactcactct acaacgcgaa
    15241 ttcgagcgcg ctcgtcaaca gggtgcgcta gcacaatgga ttgcggtttt tgaaggtggt
    15301 ttcacgggta tcgtcgccac cctggatacc ggtcgccccg gtccggttat ggctttccgt
    15361 gtcgatatgg acgcgctgga tctcagtgaa gagcaggatg tcagccatcg cccctaccgc
    15421 gacggttttg cgtcatgtaa cgccggaatg atgcatgcct gtggtcatga tgggcatacc
    15481 gccattgggc ttgggctggc gcataccctt aaacaattcg agtccggact acatggcgtc
    15541 atcaaactga tttttcagcc tgcagaggaa ggtacgcgtg gcgcgcgggc gatggtcgat
    15601 gcaggtgtcg tagatgatgt tgattatttt actgccgtgc acattggcac tggcgtacct
    15661 gcgggcaccg tggtgtgcgg cagtgataat tttatggcaa ccaccaaatt tgacgcgcac
    15721 ttcaccggga ccgccgctca cgcaggcgca aaaccagaag acggtcacaa tgccttgttg
    15781 gcggcagcac aagcaactct tgcactgcat gcaatcgccc cgcacagcga aggagcttcc
    15841 agagtaaacg tgggcgttat gcaggcagga agcggccgta acgttgttcc tgcctcggcg
    15901 ttgctgaaag tggaaacacg cggggccagc gacgtcatta atcaatatgt ttttgaccgt
    15961 gcacaacaag cgattcaggg cgcagcaacc atgtatggtg tcggcgttga aactcgcctg
    16021 atgggtgcag ctaccgccag ttctccttcg ccgcaatggg tcgcatggtt gcaaagtcag
    16081 gcggctcagg tcgcgggggt caatcaggcc attgaacgtg ttgaagcgcc tgcgggttcc
    16141 gaagatgcca cattaatgat ggcccgcgtg cagcaacatc aagggcaagc ctcctacgtg
    16201 gtgtttggca cacagctggc ggcaggtcat cacaacgaaa aattcgattt tgacgagcag
    16261 gttctcgcta ttgccgtcga aacgctggcg cgcaccgcgc tcaattttcc ctggacgcga
    16321 ggtatctgat gcaggaaatc tatcgtttta tcgacgatgc gattgaagcc gatcgccaac
    16381 gttataccga tattgccgat caaatctggg atcatccaga aacacgtttt gaagagttct
    16441 ggtcagcgga gcatctggct tcggcgctgg aatctgcaag cttcaccgtt acctgcaacg
    16501 taggcaatat cccaaatgca tttattgctt cgtttggtca aggcaaaccg gttatcgccc
    16561 tgctgggaga atatgacgcc ctggcaggtt taagtcagca agctggttgc gcgcaaccta
    16621 catccgcgac gcccggtgaa aatggtcacg gttgcggaca caatttgctg ggaaccgccg
    16681 cctttgccgc tgcaatagcc gtcaagaaat ggctggaaca atatgggcaa ggcggcacag
    16741 tgcgctttta tggttgtcct ggcgaagaag gcggctcggg taaaacgttc atggtccgcg
    16801 agggggtatt tgatgatgtg gatgcggcac tcacctggca cccggaagcc tttgccggca
    16861 tgttcaatac ccgcacgctg gcaaacattc aggcatcatg gtgctttaaa gggatcgcag
    16921 cacatgccgc gaattcccct catttgggac gcagcgccct tgatgccgta acgttgatga
    16981 ccactggcac caacttcctc aacgaacata ttattgaaaa agcgcgcgta cactatgcca
    17041 tcacaaatag cggcgggatc tcgcccaacg tggtccaggc gcaggcagaa gtgctttatc
    17101 ttatccgcgc ccccgaaatg accgacgtgc agcatattta tgatcgggtc gccaaaatcg
    17161 ccgaaggtgc ggcattgatg accgaaacca cggttgaatg ccgcttcgac aaagcctgtt
    17221 ccagttatct cccgaatcgc accttagaaa atgccatgta ccatgcccta tcccattttg
    17281 gtaccccgga atggaactcc gaagaactgg cttttgcgaa acaaattcag gctacgctca
    17341 cccccaacga tcggcaaaac agtctgaata atatcgccgc aaccggtggc gaaaacggca
    17401 aggcttttgc actacgtcat cgtgaaacgg tactggcgaa tgaggtcgct ccatatgccg
    17461 ccaccgataa cgtgcttgcg gcatcgactg atgtcggcga cgtcagttgg aaactgcctg
    17521 ttgcccagtg tttcagcccc tgttttgccg tcggtacacc gctacatacg tggcaactgg
    17581 ttagccaggg gcgaacatct attgctcata aaggaatgct gctggcggcg aaaactatgg
    17641 cagcaaccac agtcaatctc ttcattgatt cagggctatt gcaagaatgc caacaagagc
    17701 atcagcaagt tacggacacg caaccgtatc actgccctat cccgaaaaac gtgacaccgt
    17761 cacctttaaa ataacaacaa caacgcaaac acaacaaccg aggaatgccc atgagtatgt
    17821 catccatacc gtcgtcctcc caatccggga agctctatgg ctgggtcgaa agaattggta
    17881 acaaggttcc ccatcctttt ttgctcttta tctatttgat aatcgtactc atggtgacga
    17941 cggcaatttt gtcagccttt ggcgtcagtg cgaaaaaccc gaccgatggt acgccggtgg
    18001 tggtgaaaaa cctgctcagt gtggaaggat tacactggtt tttacccaat gtgattaaaa
    18061 actttagcgg ttttgctcca cttggagcga tcctggcgct ggttttaggt gccggcctag
    18121 cggagcgcgt cggcttactg ccagcgctaa tggttaaaat ggcatcgcat gttaatgccc
    18181 gctacgccag ttatatggtg ctgtttattg cttttttcag ccacatttct tccgatgcgg
    18241 cgttagtgat catgccaccg atgggtgcgc tgatttttct ggcggtgggc aggcatccag
    18301 ttgcaggttt actggctgcc attgcaggcg taggttgcgg ctttacggct aatttactga
    18361 ttgtcacaac cgacgtgttg ctgtcgggga tcagcacgga agcggcggct gcgttcaatc
    18421 cgcaaatgca cgtcagtgta attgataact ggtattttat ggccagctcc gtagtcgtgc
    18481 tgacgattgt tggcggcttg ataaccgaca aaatcatcga gccacggtta ggtcaatggc
    18541 agggaaacag cgatgagaaa ctgcagacat tgaccgaaag tcagcgtttt ggtttacgca
    18601 tagcaggtgt cgtatcgcta ctttttattg ctgcgatcgc gctgatggtg atcccggaaa
    18661 acgggatatt gcgcgatccg attaatcaca ccgtgatgcc atcacccttt attaaaggta
    18721 tcgtgccact aatcattctt tttttctttg tggtttcgct ggcttatggc atcgctaccc
    18781 gcacaattcg acgtcaggcg gatttaccgc atttaatgat tgaaccgatg aaagagatgg
    18841 cgggatttat cgtgatggtt tttcccctcg cccaatttgt cgccatgttt aactggagca
    18901 acatggggaa attcatcgcc gtggggctga ccgatatact tgaaagttca gggcttagcg
    18961 gcatcccggc gtttgtcggt ctggcgttgc tttcctcttt cttatgcatg tttattgcca
    19021 gcggttccgc aatctggtcg attctggccc ccattttcgt accaatgttt atgctacttg
    19081 gctttcaccc ggcatttgcg caaatcctct ttcgtattgc cgactcatcc gtattgcctt
    19141 tagcgccggt atctcctttt gttccactgt ttcttggatt cctgcaacgc tacaaaccag
    19201 acgcgaaact gggtacttac tattcgttag tcttgcccta tccgcttatc tttttggtgg
    19261 tatggctgct gatgttgctg gcgtggtatc ttgttggtct gccgataggt ccggggattt
    19321 acccacgttt gtcttaagag agaacggatg ctgagattac ttgaagaaaa aattgccacg
    19381 ccactgggtc cactgtgggt gatgtgcgat gagcaatttc gcctgcgggc ggttgaatgg
    19441 gaagagtaca gcgaacgcat ggtgcagctg ctggacatcc attatcgtaa agaaggctat
    19501 gagcgcattt ctgccaccaa cccaggcggt ttaagcgaca agcttcgtga ttattttgcc
    19561 ggtaatctta gcattattga tacgcttccc actgccacag gggggacgcc atttcagcgc
    19621 gaagtctgga aaactctacg cactatcccc tgcgggcagg taatgcatta cggccaactg
    19681 gctgaacaat tgggccgccc tggcgcggcg cgtgccgtag gtgcggcaaa tggatcgaat
    19741 cccatcagca tcgtcgtacc ttgccatcgg gttattggtc gaaacggcac catgaccgga
    19801 tatgcaggcg gagttcagcg aaaagagtgg ttattgcgcc acgaaggtta tcttttgctg
    19861 taaacattaa acaatttgtg ccagcttgtt cacactttta tgtaaagtta cccttaacaa
    19921 cttaagggtt ttcaaataga tagacatata tttacatcta atatcggaat tctctgctgt
    19981 taaggtttgc ttagacttac ttgctcccta aaaagatgtt aaaattgaca aatatcaatt
    20041 acggcttgag cagacctatg atcccggaaa agcgaattat acggcgcatt cagtctggcg
    20101 gttgtgctat ccattgccag gattgcagca tcagccagct ttgcatcccg ttcacactca
    20161 acgaacatga gcttgatcag cttgataata tcattgagcg gaagaagcct attcagaaag
    20221 gccagacgct gtttaaggct ggtgatgaac ttaaatcgct ttatgccatc cgctccggta
    20281 cgattaaaag ttataccatc actgagcaag gcgacgagca aatcactggt ttccatttag
    20341 ctggcgacct ggtgggattt gacgccatcg gcagcggcca tcacccgagc ttcgcgcagg
    20401 cgctggaaac ctcgatggta tgtgaaatcc cgttcgaaac gctggacgat ttgtccggta
    20461 aaatgccgaa tctgcgtcag cagatgatgc gtctgatgag cggtgaaatc aaaggcgatc
    20521 aggacatgat cttgctgttg tcgaagaaaa atgccgagga acgtctggct gcattcatct
    20581 acaacctgtc ccgtcgtttt gcccaacgcg gcttctcccc tcgtgaattc cgcctgacga
    20641 tgactcgtgg cgatatcggt aactatctgg gcctgacggt agaaaccatc agccgtctgc
    20701 tgggtcgctt ccagaaaagc ggtatgctgg cagtcaaagg taaatacatc accatcgaaa
    20761 ataacgatgc gctggcccag cttgctggtc atacgcgtaa cgttgcctga tttttccgca
    20821 taactcacta tccttctgtc atatcattaa atttttctga tttattgatc tggcagaagg
    20881 ttcatcactg tttcattcac cagatatggg ttaatctttt aattacaaac tgcgttgaca
    20941 gttgttgtaa ggagaccctg tatggctatg tatcagaaca tgctcgttgt tatcgatcct
    21001 aaccaggacg accaaccagc attgcggcga gctgtttatt tacatcaacg gattggtggc
    21061 aaaattaaag cctttttgcc gatctatgac ttctcatacg aaatgaccac cctgctctcc
    21121 ccggacgaac gtaccgctat gcgtcagggc gtcatcagcc agcgtacagc ctggatccac
    21181 gagctggcaa aatattatct caatgctggc gttcccattg aaattaaagt ggtctggcat
    21241 aaccgtccct tcgaagccat cattcaggaa gtgatcagcg gcgggcacga tttggtgcta
    21301 aaaatggcgc accaacatga ccgtctggaa gcggtgattt ttacgccaac ggactggcat
    21361 ctgttacgca aatgcccaag cccggtgtgg atggtgaaag accagccgtg gccggaagga
    21421 ggcaaggcgc tggtggcggt gaatctcgcc agtgaagagc cgtatcataa tgcgctcaat
    21481 gaaaaactgg tcaaagagac gatcgaactg gcagaacaag tcaaccatac cgaagttcat
    21541 ctggttggcg cttatccggt aacgccaatt aatatcgcga ttgaactgcc ggaatttgat
    21601 ccgagcgttt ataacgatgc cattcgtggg caacatttgc tggcaatgaa agctctgcgg
    21661 cagaaattcg gcattaatga aaacatgacg cacgtagaaa aaggtctgcc agaagaggtg
    21721 attcctgatt tggcggagca cttacaggcg gggattgtgg ttctgggcac ggtaggacgc
    21781 accggtattt cagcagcatt cctcggcaac acggcggaac aggtgattga tcatcttcgc
    21841 tgcgatctgt tggtcattaa acctgaccag tatcagacac ccgttgaact ggatgacgaa
    21901 gaagacgatt aacgactctt caataactga caaggcccga gttatcgggc ctatttttta
    21961 cttactgctc ttcgccgcca aggaaataaa ttcccaacgg gattgccagc aacacagtaa
    22021 agaccaggct ataaacaaaa atcatcgatg actgaatcac atacattgat gtcgtccact
    22081 cagacagcgg aatattgtac tgttctataa cgccgccgat agtcgcgcga gtcacaaccg
    22141 acgccgcaat aatgaaaacc gccaacaaac caaagagaaa tttcttccct ttcgctgact
    22201 tcagtttcgc cataatcatg atgtaatcct tgtgcagaca aatttcttgt tacctaaacg
    22261 taacaatacc atgacatttc accgttgtct gctctgaaaa cctcacctct tagctggatt
    22321 tacaatcaac gcattgatta gttagctaat gttgctactt ccttccctga ctattgatga
    22381 tctgaatgaa ttgtcgcggt aaaatagccc cgccaggggt ttccggcatg acgacaatta
    22441 tctggaggag cctgataaaa caatgatcgc tgaactgttt acaaataatg cgcttaatct
    22501 ggtcattatt ttcggtagct gcgcagcatt gattctgatg agcttttggt ttcgccgcgg
    22561 aaatcgtaaa agaaaaggtt ttttattcca tgcggtgcaa tttttaatct acaccataat
    22621 tatcagtgct gttggtagca ttattaatta tgttatagaa aactataaac tcaaatttat
    22681 caccccaggc gttatcgatt ttatctgtac gtccctgatt gcggttattc tgacgattaa
    22741 gctatttttg ctgattaatc agtttgaaaa acagcagatt aaaaaaggtc gcgatatcac
    22801 cagtgcgcgg attatgtcgc gcattatcaa aatcaccatt attgtggtgc ttgttctgct
    22861 ttatggcgaa catttcggca tgagcctttc tggcttgctg acctttggtg gtattggtgg
    22921 tctggctgtc ggtatggccg gtaaagatat tctgagtaat ttcttttccg ggatcatgct
    22981 ctatttcgac cgtcctttca gtattggcga ctggatccgt tcaccggaca gaaatatcga
    23041 aggtacagta gcggaaattg gctggcgaat taccaaaatt acgacctttg ataatcgtcc
    23101 attgtacgta ccgaactcgc tgttttcgtc gatcagcgta gaaaacccag gacgaatgac
    23161 caaccgccgc attaccacga ccattggttt acgttatgag gatgcggcaa aagtgggcgt
    23221 tattgtcgaa gccgtacgtg agatgctgaa aaatcacccg gccatcgacc agcgacaaac
    23281 cttactggtt tatttcaacc agtttgccga ctcgtcattg aatattatgg tttattgctt
    23341 taccaaaacc acggtatggg ctgaatggct tgctgcacag caagacgttt atttgaagat
    23401 tatcgatatt gtgcagtcac acggcgcgga ctttgccttc cccagccaga cgctgtatat
    23461 ggataacatt acaccgccgg aacaggggcg ctaaatcaga catcactggc gttatccaca
    23521 agggtaacgc cagttcccat cacatcaatg cttcacaata tacatagtcc ggctgtacgc
    23581 aacatcttcc gggttattaa tgggataacc tttcagccac ggcttgatta atcgtccatt
    23641 ggtatattga taaattggtg caatcggtgc ttgctccatg aggatttttt ctgccgcgtt
    23701 gtaatcggca ttacgcgctt taacggtatt ttccgtcgat gcctgggcca gaactttgtc
    23761 gtatgccgga ttgttaaagc gtgaaatatt tcctgaatgc gttgacgtta ataatgtcag
    23821 gaaagtggag ggttcattat aatcccccac ccatgaggcg cggataacat caaaattgcc
    23881 ggtgttacgg ctatcgatat aggttttcca ttcctgattt tgcaatttaa catctacgcc
    23941 aaggttcttt ttccacatcg atgctacagc aatcgcaatt ttttgatggt tttctgaagt
    24001 gttatacaaa agcgtcagct tcagcggttt ttgcggacca taaccagctg cgctcaacaa
    24061 agtttttgcc tgcgcattca gttcttcctg actcatttgt tcaaacggcg aaggttccgg
    24121 cgtaaatccg gcggtaacat ctggtgtaaa atgccacgct ggcttttcgc ccgttcccaa
    24181 cactttttcg gtcatcaggc ggcgatctat cgtcatactt aatgccaggc gaacgcgctg
    24241 atctgccgtc ggcccttttt gcgtgttaaa cgcataataa taggtcccga gctgtggcgg
    24301 cgtataaacc tgccccggaa tatccttcaa cagcttctga tacatatttt tcgggaagga
    24361 ttcggtgata tcaatatccc ccgcgaggta acgcttagtg gctgcggatt cctgattaat
    24421 tggcaggaag gtcacttttt gcagtaccgt tttggcgtta tcccaataat gggtattcgg
    24481 taccacgacc agtttttcat tgactacgcg atctttaaga acataagcgc cattgccgat
    24541 cagatttccg ggtttcgtcc actctttacc gctttctacg ttggcttttt gcaccgggaa
    24601 gaaggcaaag ttagcggtta aattcacaaa ccacggcaac ggtttatcaa gctgaatttt
    24661 caaagtatgg gcatcaactg cggtgacgcc aagctggtct ggcgtagctt taccatcaat
    24721 aatcgcctgt gcgttgttga ttcccgccag cgcggcaaac catgcaaatg gcgatagcgt
    24781 tttcgggtcc accagacgtt gccagctgta gacaaaatct tgcgccgtta ccggtgtgcc
    24841 atccgcccat tttgcgttat cgcgcagggt aaaagtccag atacggttgt cattactttt
    24901 ccactgagtc gcaacgccgg ggacaatctc ccctttctcg ttctgattca ccagtccttc
    24961 aaacagatcg cgaatgacct gaatctctgg caggcccacg gctttagcgg gatccagcga
    25021 cgcaggctca tctttaatgt ggcgcaccag ctcctgcttc tctgccagta ctgtgccgct
    25081 cggaacttct gcagcatacg aaagagaaat gctgctgacc aacagcgcac aacacgtgac
    25141 tgaaacagag tgcttcataa gtttccctct aaccgacatt aaaattgatg cgtaattatt
    25201 tgtttcgcct taaagaaatg caaacaactt ccatcaaaaa agtgacacag gaagcataaa
    25261 atgttttatg tatcaatata ttaaatcaga tttagtgtaa ataatcgacg ccaaaatgcg
    25321 gtaaagcaca tagaacaccg aacactacgt tcaggcttcg cacgttcaaa ccagttttcg
    25381 gtatcataat cagcatctgt cgccattgta cgaagaataa aaaataacca aaacgttgaa
    25441 aggaactgag aatacgttaa gagagaaatt aaattaatgt atcagcaagt agtgctaaac
    25501 agcaccttta tacttcagcc tatctataaa taatgagaat gccgagccag catgtcgacg
    25561 gtgtggatag tacaggtgat aaccgggtaa atctggtgtg aatttatcca aaacacgtat
    25621 cagttttttt tctttaatag cccgctcaac ctgatcataa ggtagatacg ccaatccatg
    25681 cccatcaatt gcagcatcaa tgatcaggtc tatcgtattc agtaaaagct gaccttccat
    25741 gcgaacacga acttcacgtc caccgcgtat taatctccag cgatttgctg tacccgatgt
    25801 gggaagatac aaattaattg cctgatgatc tattaattgt gacactgacg ttggaacact
    25861 tcggcgagaa aaataatccg gtgagccaac aatagccatt ggtatatctg gcccgattcg
    25921 aatggcgatc atatctttat ccatctcccc acccagacgg acgcctgcat caaaacgttc
    25981 agaaacgaca tcggtcaaac cataatcaat ggtgagctga atatcaattt caggatgcga
    26041 tttcaggaat gtgcgcattg ctggtaacaa tatcgttttt gctgcatgtt ctacagtagt
    26101 aatacgtatt gtcccggatg ggcggttctg cagctcgctc agggatgcca gggctgaatc
    26161 tatgtcatgc aacatagggc caagaacaga caaaagatgc tcgcccgctt cagttggaac
    26221 aacgctgcgc gtggttcgcg tcagaagccg caatcccaat cgttcttcta tacgacgcac
    26281 tatctggctt aaagctgact gcgccatgct caggcgggct gctgcacgag tgaagctacg
    26341 ctcctctgca acgacgacaa acgccatcag atcagcgatt tcttcacgct tcattcagta
    26401 tctccatgca atctattaat catttaaatg ataggtctaa tcatgatttt gggtctaatc
    26461 aacttaatga tcaatatcta tagtgatatc accaaccgtt aaaacgaacc gacaactcca
    26521 tgaggaacgg aatgatgaat aataaagtca gcttcactaa cagcaataat ccaaccatct
    26581 ctttgtctgc agtgatctat ttccctccaa aatttgatga aacccgtcag tatcaggcaa
    26641 ttgtggtatc tcacccaggt gggggcgtta aagaacaaac ggccggaacc tatgccaaaa
    26701 aactggcgga aaagggattt gtcactattg cttatgacgc atcttatcaa ggtgaaagtg
    26761 gcggcgagcc gcgtcagtta gaaaacccat acattcgtac tgaagacatc agtgccgtga
    26821 ttgattacct gacaacgctt tcttatgtcg acaatacccg aattggtgcg atgggaattt
    26881 gtgccggtgc aggatataca gccaatgctg caattcagga tcgccgcatc aaggccatcg
    26941 gtaccgttag tgcggtcaat attggttcga tattccgtaa tggttgggaa aacaatgtga
    27001 aatctattga tgccctgcca tacgttgaag cgggttcaaa cgccagaacc agcgatatta
    27061 gcagcggaga atacgccatt atgccactgg caccaatgaa ggagtccgat gcgccgaacg
    27121 aagaactgcg tcaggcctgg gagtattacc acacccctcg cgcgcagtat ccaacagcac
    27181 cgggttacgc tactttgcgc agccttaacc agattattac ctatgatgct taccatatgg
    27241 cggaagtgta cctgactcag ccgacgcaga ttgtggcggg cagccaggca gggagcaaat
    27301 ggatgagtga cgacctgtac gatcgagcat caagccagga taaacgctat cacattgttg
    27361 aaggcgcaaa ccatatggat ctttatgacg gcaaagccta tgtggctgaa gcaatttcag
    27421 tattagctcc gttctttgag gaaactctgt aatgaaacat attcaaatcc gcaatagtga
    27481 tatggcctgg catattgctg ccaatattca gtttccacca aattttgatg agtcaaaaca
    27541 gtatccggcg attatcagcg tccatccttt tggcagttgt aaggagcaga cgtctggcaa
    27601 tatctatggc aaagcattgg ctgaaaaagg ctatctcgtt ctcgcttatg acgccagttt
    27661 ccagggagaa tctggtgggg agcctcgctg gatagaagat ccgacacagc gcgtagagga
    27721 tattagccgt gttatcgatt atgcagtaac tctgccatat gttaatgctg aacgtattgg
    27781 cgtgctggga gtgtgtgggg gtggtggcta tgccattaat gccgcgctga cagaaaaacg
    27841 aattaaagca gtagtgagta ttacgggtgt taatatcggg cgtctttttc gggaaggttt
    27901 cagcaattac gatccgattg gcgcattgaa tgcaatggcg tcgcaacgaa ccaaagaggc
    27961 tcgtggtgga gaattacaga tcaacgaatt acttcccgcc agcctggatg cagcaaaagc
    28021 ccatggactg accgaacgcg atgtttatga agcaactgac tactataaaa caccacgcgg
    28081 gcaacaaccg ggaggtgcga caaaaatgtt gttctcacat gcacagaaaa cgctggcctg
    28141 ggatgccttc gcatttactg aagtgttact gactcagccg gtgatggtag tagttggtga
    28201 aaaagtcggt gccttcggtg cttatcgtga tggactggaa gtctatggcc gggcgatggt
    28261 ttcgcaggat cgtcagttag tttcattacc cgatttctca cattatgaac tgtatgacaa
    28321 accggaagca gttcagaagg cgctggaaaa agttattcct tttttcaaca cccatctggg
    28381 ataagtttca tgagaaatat tatgaaaaat gtactgattc ttggtgctgg cggtcagata
    28441 gcccgccatg tgattaatca actggcagat aaacaaacca ttaaacaaac tttatttgcc
    28501 cgtcagccag caaaaatcca caaaccctat ccaactaata gcaagattat tatgggagat
    28561 gtactgaatc atgcagcact gaagcaggcc atgcagggac aggacgttgt gtatgccaac
    28621 cttacggggg aagatttaga tattcaggca aattcagtga tcgctgcgat gaaagcctgt
    28681 gatgttaaac gtctgatctt tgttctttcc ctcggtattt atgatgaagt tccaggaaaa
    28741 tttggcgaat ggaataatgc agttattggt gagcccctaa aaccattccg cagggctgcg
    28801 gatgcaattg aggcttctgg actggaatat acaattcttc gccctgcctg gctgactgat
    28861 gaagatatta ttgattatga gcttacctca cgcaatgaac cattcaaagg tacgatcgta
    28921 tcacgtaaga gtgtcgcagc attaatcaca gatatcatcg ataaacctga gaaacacatt
    28981 ggtgagaata taggtatcaa tcaacccgtg actgatggtg acaagccatt attcatgtaa
    29041 atcaattaca tagttaccat cgtaatgtac tatgtgcagt acgatttatt aaacttatta
    29101 aggtgaactg atcaatccga tgcgattaac atgtgtagaa ccagtaagaa taaaatacgc
    29161 catgtatata acattacgac ttacatctaa gtcagcataa ttctaatatc tgcacatcaa
    29221 taaaaatgca aacaacttcc ggcaaaaaag tgatacaaga tcgatgacat agcgttgaag
    29281 aaaaggattt gataatccgc aaaatttgca caacactgcc tgtaatactt cctgaaccag
    29341 agacgatcat gaccgtaaac cgcccacgcg ccgaacgcgg cgcatttccg cccggaacag
    29401 aacattacgg acgttcatta ttgggcgcgc cgttaatctg gtttccggcc actgcagcca
    29461 gtcgcgaaag tggtttgatt ctggctggca ctcacggtga tgaaaactct tcggtcgtca
    29521 cgctctcctg tgctctgcgg actttgacac cttctttgcg ccgccatcat gtagtgctgt
    29581 gtgtgaatcc tgacggctgc cagctggggt tacgggccaa tgctaatggt gtggatttaa
    29641 accgaaactt tccggcggcg aactggaagg aaggtgaaac agtttatcgc tggaacagcg
    29701 ccgctgaaga acgtgatgtc gtattgctga caggtgacaa acctggctct gaacctgaaa
    29761 cccaggcgct gtgccagctt atacaccgca ttcaacccgc ctgggtggtc tcattccacg
    29821 atccgctggc ctgtattgaa gatcccagac acagcgaatt aggcgaatgg ctggcccagg
    29881 cgtttgaatt gcctctggta accagcgtcg gttatgaaac acctggctct ttcggtagtt
    29941 ggtgtgcgga tctcaacctg cattgcatca ccgcagagtt tccgcccatc tcctccgacg
    30001 aagccagcga aaaatatctc tttgcgatgg caaacttgct gcgctggcat cctaaagatg
    30061 caattcgccc gtcgtgaact gaagcgccgg ttccacatct accgccagcc aggtcggtcc
    30121 gtcaagatcg acgaaactga cctgcggcac cagcggtaaa gcggcgctaa tggcccgaga
    30181 ggtacacaac atgcagccca gcatcagacg aaaaccttgt gcacgcgctt cagtcgccag
    30241 cgccagcgct tccgtcagac caccggtttt atcaagctta atgttaacca tctcatagcg
    30301 ccctttcagc gccttcagat tgctacgtgt atgacaactt tcatctgcac aaatcggtaa
    30361 cggatgaata aaattctcca gcgccgcatc gtcctgcgca ggaagcggtt gttcaagcat
    30421 cgcaacgcct aaatccgcca atagctggca acgcgccgcc aacccttctg cacgccagga
    30481 ttcatttgcg tcaacgatca gcgtcgcatc gggcacagct gtgcgaattg ccactatccg
    30541 ctcactgata agatggttat ccagcttcac tttcagtaat ttcgcgcctg cctgccagag
    30601 tgttgatgca ctattggcca tctgatcagg cgtaccgatg actaccgtct gtgcagtggt
    30661 gactgtcccg ggaagcgtta tgccgatcaa atcagccagc gattgctgct gttttcgcgc
    30721 ggccagatcc cacaatgcac aatccagcgc attacgtgct gcgccggcag ggagaatttt
    30781 ttgcaactcc tcccgtgtca gccctttctc taattgcggc acgacgctca taatttgcgc
    30841 cattaccgag gcatcacttt ccccataacg cggatacggc gtgcattcgc cggtgccttt
    30901 aataccctct tcttccagtt caaccaccac cacgcgcgct tcactgcgac ttccccgggc
    30961 aatcacaaac ggggtatgta agggccaggc ttcctcaaat accttaacgg ttctcatctt
    31021 tactccttgt tgtcgcggtg gtctgcggat taagcgcaaa tagcgtttgc tgtgttattg
    31081 acagttagca taaactaggt gtgacgttaa ctatatgtaa acaggaaaga taatcatgtc
    31141 acaaaccgtt catttccagg gcaacccggt tacagtcgcc aattccatcc cgcaggcggg
    31201 tagcaaagcg cagactttta ctctcgtggc aaaagatctg tctgacgtca ccctcggtca
    31261 gtttgcgggt aaacgcaaag tgctgaacat tttcccgagt attgataccg gtgtttgcgc
    31321 cgcatcagta cgtaagttta accaactggc aactgagatc gacaacaccg ttgtgctgtg
    31381 tatctctgcc gatctgccgt tcgcccagtc tcgtttctgc ggcgcagaag gtctgaacaa
    31441 cgttatcacc ctctccactt tccgtaacgc tgaatttctg caagccttcg gtgtggcaat
    31501 tgctgatggc ccactgaaag gtctggcagc gcgtgccgtt gtggttattg acgaaaatga
    31561 caatgtgatt ttcagccagc tggtggatga aatcaccacc gagccggatt acgaagcagc
    31621 tctggctgta ctgaaagcat aagttaatat gcctgatggt gttgcaccat caggcatatt
    31681 cgcgcttact cttcgttctt cttctgactc agaccatatt cccgcaactt attggcaatc
    31741 gcggtatgtg aaacgccgag acgttttgcc agtttgcgcg tgctgggata attgcgataa
    31801 agctgggtta ataccgagcg ttcaaaacgg ctggtgattt cgtccagcga accttccatc
    31861 gcatcttcgc ccacggctac cgttgcggcg tcataatccg gcaacaaaat atcctgtgga
    31921 cgcagctcat aaccgtccag ttgtgtcagt gcgcgataga tagcgttctt taactgccgc
    31981 acatttcccg gccacgcata acgcgtaagt acagtattca ggtcagcggc cagtttcgga
    32041 cgcggcacgc cctgctcgtc ggcaaagcgg gcgacgaaca gctcagttaa cggcatgatg
    32101 tcctgcggac agtcacgtag cggcggcaga ttgagcgtca acacgttcag acgataatag
    32161 agatcttcac ggaacatgcc tttttgcacc agttcgacca gattcttctg cgtagcgcaa
    32221 atcacccgca catcgacatg cacctcatgg tcttcgccaa cccgacggaa agtgccatca
    32281 ttaaggaaac gcagtaattt cgcctgcatc cgtggtgaca tttcccctat ttcatccaac
    32341 agcaccgaac caccgttcgc ctgctcaaag aatcctttct tcccttccgg agcatgacca
    32401 aacagttcac tctcgaccgc atcttccggt atagacgcac agttcagcgc caggtaaggt
    32461 ttgcccgctc tggggcttgc ctgatggcag gcgtaggcaa agagatcttt acctgtacct
    32521 gtgtcacccg taatcagcag cggcgcgctt agcatcgcca gtttctgcgc ctgttcgaca
    32581 acatgcttca ttttcgggct gacggcgaca atttgactga aggcgctgac gtcctgggcg
    32641 gcgacatttt gcaactggcg gcccatacga atcgttgatc gcaacatcac caccgcaccg
    32701 gtcaggacgt gttgatcatt ttcatcctga agataaacag gcgtaatctc catcaggaaa
    32761 ttctgcccat taataacgac atgctcgtta tgggaatctt gcggttcgct ttccagccaa
    32821 cgtaaaaaat taaagccgtt aatcaattgt gcggcggtat ggttgcgcag gcgatccaat
    32881 ttttgcgcaa aaagctgaca gctcgccggg ttcgccatat ccactttgct tttcatatcg
    32941 acagagagca caggttcggg caacgcctcc agtaacgcgc tcaacgccag atgctcacgt
    33001 tcggaaggca tccacgggac agtacgcaca tcggtaacac ctgcaatacg gcgtatttcg
    33061 gccatcagac tgctgaaact ctcaaactcc agttcagcaa aattgaggta gattcgccca
    33121 atgggatcaa tctcaatacc gcgtaaatca atgcctctta gcacgagtag atcgagtaat
    33181 tcgcgggtca gaccgagtcg gtcttcacaa aagacttcca gacgcatggg aaccttcacc
    33241 tgaaaaaaga acaattaata tgatgatatg acactatttg gatagcagga aggtttctgt
    33301 caacaatcat tgacacaaag acggaaaaac gggcacttcg gcgtaaagat atccggtcag
    33361 cacccgatat tgcattattt ttcgctgggc gttttgcctt tttgcagcgt ttctttcacc
    33421 tgaccgataa gctgacgacg gaaatccccg aggcgaggtt tgtcatcgtc aatccacggc
    33481 agcgggcggc aaagctccat agctttaatc ccgagtcgtg ccgtcagaag tccagcacca
    33541 attccctgag ctgcgcgggt agacaaacga gcagcgagat cttgcgacat ccagtccatc
    33601 cccacttcgc gcaccagttc gctggctccg gcaaaagcga tattcagcaa taccagctta
    33661 aacagacgca aacggctgta ataccccagt tcaatgccat acagcgtggc gatgcgatta
    33721 attaaacgca gattgcgcca ggcgataaac gccatatcga ccaacgccag cgggctgacc
    33781 gcaatcatca acgttgattc cgccgccgaa cggctgattt cgcgccgcgc ctgggcatct
    33841 aaaactggct ggaccaaatg cgcatacaaa ctgaccactt cacggtcgtt ttgcgtttca
    33901 tggattgagg cataccagcg ttgcagcgct ggatgagact gatcaatacc cgcctgctgc
    33961 gccagttttt cgcaaaatgc gcggcctttg cccgtgccgt ggctgtgcaa caaatcgcgc
    34021 gcttcgtcgc gttcatgggc gcgctgtcgc aagcgccata agcgccgcca ctctgttacc
    34081 acagaaccta cgccagcgcc gataatcaat gccccagcgg cacatccacc cagcgccacc
    34141 cagtcctgag tttgccaggc attcattgtc cactgtacac cctgcccgac aacgcttgcg
    34201 ccaaacagag ccagcccgcc catcaccatt ttgcgccaca ggctgcgttt cggacgtaac
    34261 gctgcatcca ttaccgcttc aacttgcccc tcttcttcag gcgcttcatc gagcgtggcc
    34321 ggggcaaaat tttgcgcctg attttcgtca aaggtctgct gcgccctgaa ttttggattc
    34381 tgatcgacct ccagcggacc gtcgaaatca atacgtggtt ttaacggttc ggtcatcgca
    34441 atttatctcc tattaaaaat tcaagcgcgg catcaagacg aatatgcggc agcggtttgt
    34501 cgacatccat cacctgcggg cgaaacgctt caaactggaa cccttgcttg tcccagaacg
    34561 cctgaccggg caaacgcgca ggaacttcac cgggataaac ggtgagtggc gcaccatcgc
    34621 tgagccggtt accgcgcaat gccggaattt tctcgccgtt gacgtcaata atgccgctgg
    34681 tggtcgcctg caccgaggcc agccccaggc aatccatact aatcccttca aacgccgcat
    34741 tttgccaggc atcctgaatc agttgttgca gtaatgaaac catattggcg tgctgatcga
    34801 tggtcacatg gtccgcttta gtggcagcaa acaatagctt atcgataacc ggcgaaaaca
    34861 aacgccggaa caaggtacgc tgcccgtagt ggaaactttg catcagctgc gtcagtgcca
    34921 gacgcatatc attaaatgcc tgtggcccac tgttgagagg ttgcaggcaa tcaaccagca
    34981 caatctggcg gtcaaagcgc agaaaatgat tcttatagaa ccccttcacc accttctcgc
    35041 agtaataatt aaaccgctcg cgcagcattc cggcattggt gtgcttatcg gcctgcgcca
    35101 gtttggactc gccccaggca tcgacatccg gccacgggaa gaattgcagc gcgggcgcac
    35161 ctgccatatc tcccggcaag acaaagcgcc caggctgaat aaagtgcagc ccctgctgtt
    35221 tacagtggtg gagataatcg gtccacgcgg cggcaatgtc tgccagccgg ttttcgtcgg
    35281 caggcgctag cgggtccagc ccttcgcaca tcattcgcca tttgaccgac cattcgccgc
    35341 gctgaccatt gagtaagccc gtcatctggc gtgaccagct taaatagtcc tgcgccagca
    35401 tcggcaggtc gagcaaccat tcgccgggat aatccacaat ctccagatac agcgtggagg
    35461 tgtccttgaa gtggcgtagc agcgaatcgt tcgatttaaa acgtagcgcc aggcggattt
    35521 cactgacgcc gcgcgttggc gtcggccagg cgggaggatc gccatataac tgcgccagtc
    35581 cttcatcata tgtgaagcgc ggaatgccaa agtcacgctg aggaatgcgt tttacgccca
    35641 gcaggcgctc ttcacgcacc gcgcttaaca gcggcaagcg tgctccggcg tgaatattga
    35701 gcaactgatt gaccatcgca gtgataaacg ctgttttgcc gctgcggctt agtccggtta
    35761 cggcgaggcg cagatgtctg tcgacacccc gattcaccag cgcattaagt tcatttttaa
    35821 gtcgcttcat tgctgtcctg tgctgctctg aagaccagaa aaaaatcatt cgtaaggata
    35881 tgaatgtggg ggccaggcca ttgattatca actgcccgtg ttgtcatcga cgggcgaact
    35941 ccataagatg tgcagcattc acgttcggac ggagttcctg acctatgtcc cctactattt
    36001 atgatattgc cagggttgca ggcgtatcaa aatccaccgt atcacgcgtg ctgaataagc
    36061 aaactaatat ctccccggaa gcgcgcgaaa aagtgttacg ggccattgaa gaattacagt
    36121 atcagccaaa caagctggcc cgcgcgctga cctcttcggg ttttgatgcc attatggtga
    36181 tttctacccg ttcgaccaaa actacggcgg gtaatccgtt tttctctgaa gttttgcatg
    36241 ccatcaccgc caaagctgaa gaggaaggtt tcgacgtgat attgcagacg tcgcacaatc
    36301 cggcagaaga cttacaaaaa tgtgaaagca aaattaagca gaaaatgatt aaaggcattg
    36361 ttatgctaag ttcgccagcg gatgagtcat tttttgccca acttgataaa tacgatattc
    36421 ctgtcgtggt gattggcaaa gttgaaggtc aatatgccca tgtttattct gtcgataccg
    36481 ataattttgg cgacagcatt gcgttgaccg atgcattaat tgaaagtggg catcaaaata
    36541 ttgcctgcct gcatgctccg cttgatgtcc atgtttcagt ggatcgggta aatggttata
    36601 agcagagcct ggctgcgcat aatattgcag tgcgtgatga atggattgtt gacggcggtt
    36661 atacccatga aacagcactg caagccgcgc gggaattatt aagccagtca ccgttgcctg
    36721 aggcagtgtt tgctactgac agcctgaaat taatgagtat ttatcgtgcg gcagcagaga
    36781 aaaatattgc tattccgcag cagttagcgg tggtgggtta tagtaatgaa acgctgtcgt
    36841 ttattttaac gcctgcgcca ggcggtattg atatcccaac acaggagtta ggtcaacaaa
    36901 gctgtgagtt attattccgc ttaatttctg gaaaaccgtc accacagaat attaccgttg
    36961 ccacgcatat gacgctgaaa taaataccag ctaccttgcc tgaatgccgg atgcgatgct
    37021 agcgcatctt atccggccta catttatagt tcggataaag tactcgtgcc gcatccggca
    37081 atttagccca ttatcagaac gagtaattta cgccgacacc tgcataatgg aatttatcgc
    37141 tgtcgccttc gtcgtgatcc tgccactcaa acgcgtattc cagcgaaacg gaaagcccgt
    37201 tctggaaatc ataaccgtaa aataaaccta cacggttaaa atcatggcct tcacgttcaa
    37261 tatcatcctg ccagtcccag ttactccagc gatccagccc aatgcgcgta tacggcgtca
    37321 ccgagtggtt gccgagcgtc agcggcaaat aggcgcgaat ttcttgcgta gaaaactcac
    37381 cgttattgcg gctgtcgtcc atattaaagc cgcgctcgag ataatagttc actcgcaagg
    37441 caaccgtttc gttgaaggta tattgcaggc ctgtttccgt ttcgactcgg gtatcagcgt
    37501 aaccggtcgt gttcagatcg ttggcaaatt tatacatcga caaccaaccg ttgaaacgta
    37561 aatcgtcagt cagtttcaca tcccagtctg gcgcgatttt ccagcgctgc atattcgccg
    37621 tgtctttacc cggttcatca acgtagtgat aaccataatt acggaaaccg ccggtcaggc
    37681 cgaaactgaa atcatcgttt tcgaggaact gataatgcac ctccagctcc gggcgatcaa
    37741 accacgttcc acgtttaccc gcgctataat ctaccggccc ttcctgataa taggccagag
    37801 caattctcca cggcccgttg gcggcattaa aatagactga aggctccgcc agcccatcca
    37861 tatcttcgcc ataaccctcg acgttttcta tttcgtacat cgcgccgata ttaaagtgcc
    37921 agtcgttcct ttcctcggcc tgtgcgcagg ccattcccgc acacatcacc agtgcggtac
    37981 agggtaataa ctttttcatt attgttcctt gtattattct gggggaatgc cttccccctg
    38041 tatttagcga attgctattt ccgtttctgc atcaaagaaa tgacatttcg tcatatcaaa
    38101 atgaatagtg atattttctc ctgcatgata atcatttaac gcaccagcac ggaccactaa
    38161 ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca ccggttaatt ctgccacgct
    38221 aattttggcg gaaatgttat tttcctcttg cgcgtccgga tgaatatctt ccggacgtat
    38281 tcccatcacg atgggcttat gcaaacttcc ctgtgttttc agaaccgcta atttctcttc
    38341 gggaatggtt aatttaagcg tttccgtaac gaatttatcg ccatcgatcg tgccacgaat
    38401 aaaattcatc gccggtgatc caataaatcc ggcaacaaac atattcgctg gttggttata
    38461 aaccgttttc ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt
    38521 cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt
    38581 ctggtgtagc ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga
    38641 gagcggttca tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac
    38701 acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc
    38761 gagaatttgc gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat
    38821 cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg
    38881 gaacaccatc gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat
    38941 caacagatcg ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt
    39001 accgcagccc gacgtgccga caaacacgat gaactcttta tcggcaattt ccaggttgaa
    39061 gtccttcacc acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg
    39121 agccatgttg attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca
    39181 gagagtccgt tgaaggcaac agtaattgcg ccccggttaa gcccgcgccg atccccaccg
    39241 agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac
    39301 atgcctgcgg cggcacgccc agcccggcac aggcggcgag aaagatttcc gggtccggtt
    39361 tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa aaactcgcgc agctccagcg
    39421 ccgctaaaat cgtcggcgca ttcagggaga cagaagccag cccaaccggg atctgctgtg
    39481 cacggagatc tgccagcaaa gagcgaatgc cgggtagaac agcgctgacc gtcaactcgc
    39541 gcagtgagtg gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt
    39601 taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga
    39661 tcccttttag ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct
    39721 gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa
    39781 ttaccccttg cagtttcatt catcctcctg atgtgtggta gcggtcccat tgaagggcaa
    39841 aataaaatca cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa
    39901 acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg
    39961 taattcgcag ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc
    40021 cggattgaga tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc
    40081 cagccagata gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc
    40141 accaagatcg atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca
    40201 gcgtgcggca acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg
    40261 ttcataaaat tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta
    40321 attgagcatc accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga
    40381 ataatccagc agaatggttt gcttccccgc cgccgctttg tatttcgcca gattaatcgc
    40441 cggcttagcc ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag
    40501 ccgcagttct ttaaggaaca tttcggcgcg atggataaac gcatcgtcgc tacagccaaa
    40561 ctggcgggca atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc
    40621 gttattattg acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg
    40681 gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat
    40741 gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca
    40801 ggcgatatcg gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc
    40861 ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc
    40921 ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa
    40981 gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa
    41041 cggtaacaaa aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa
    41101 gccttttgcc gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata
    41161 aagcgcataa tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg
    41221 tttctgccac cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg
    41281 ctacgcgcac atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg
    41341 cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg
    41401 aagctgcgca ctggtatgtt gctgcaaacg gcgctctttg gcggtatagc attgctgcac
    41461 atcaccgctc accatacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt
    41521 gtagatcccc tgcatcagat gctgaccaaa cacccgcacc tgggtttcgt cgagatgttg
    41581 gcgaccgtgg ttggtttgcg tggcgtcgat acctgttgaa atcagcactg acgcgtcggc
    41641 gtccagtggc gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa
    41701 gcgacggctg gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg
    41761 taattctcca ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac
    41821 ctcaccattt atggcaatct ccatccccaa gatatcaggc aggttcacca gttcgttgat
    41881 ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt
    41941 gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga
    42001 tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt
    42061 cataattcca cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg
    42121 atgatgtacc cctgctcggc gtcggctatc atcacgggct tgccctgtac gtgattgata
    42181 aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca gttcccgttg catcagcgtc
    42241 attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accggcttta
    42301 tcaccacaaa agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt
    42361 tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaataacga atcttcgacg
    42421 ctgtaagttg ccggatccca ctcgccaaat tgaccgcagc tcttttgcgt gccgatcttt
    42481 tgaaagctat gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgccgca
    42541 tccagcatat gaatgccgat gtcgataagc gggccaccac cctgcagttc tttattggta
    42601 aagacacccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc
    42661 tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacgg
    42721 tggtgaaagt cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc
    42781 gcctgttctg gcgtcatggc gggcggtttt tcgcacatca catggcagcc cgcttccagt
    42841 gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt
    42901 ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg atgcattacc gtatttatct
    42961 gccagcgcct gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg
    43021 cgggtgcagt agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg
    43081 acccgcagcg gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac
    43141 caggccaacg aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac
    43201 actacatagc cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg
    43261 gtgccgctgc ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg
    43321 cggttgtcat gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg
    43381 ccgataatct gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg
    43441 gtgttgatca tatgatcctg atagcggttt aacggttcga gatacaccac ggttccggta
    43501 cgcgcggcga cctgttccag tacgtgcagg gaatcactca ccattttgcg gtcgccgtcc
    43561 aggctacgcg gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga
    43621 acgacgatac ctttaccccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt
    43681 aagccattaa gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag
    43741 gcggtggtca ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg
    43801 accagcagtt tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat
    43861 ttctccagaa tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca
    43921 ttcctttgaa ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat
    43981 actgcatgta gctttccggg ctcttggcaa aggtcactac cgggtcgatt aaatcttcgc
    44041 aattgagata accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc
    44101 agcgcggata atccgggttc ggttcgctgc acgcacggga gaaaacaatt ttggcgttat
    44161 tgaaatgcgc ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcgacat
    44221 aagagatggt gccgccataa gcaaggccac gcagcgccga ttgcagcgcg tcggcgtaac
    44281 cgctggtttc gataatcaca tcggcaccct gcttgccggt cagcgttttg atctcttttc
    44341 ctacgtcagt gccaatgggg ttaaggcaga aatcagcacc gtggcgacgg gcaatatcac
    44401 agcgataggc gataggatcg acgccaatca ccaccgacgc gccagcgcgt ttcgccagtt
    44461 ggatggctat ttgaccaatc gcgccaagcc ctaccaccac cacaaaatca cccacgcgca
    44521 cgttggcatc gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt
    44581 tccacgagct gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga
    44641 ccgtctcgga gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt
    44701 cgctgccgca ctcgataatg tcgccaacca ccatgttgcc aagctggaat ttgccaaact
    44761 caatgccgcg cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt
    44821 cttcatcgat aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg
    44881 cgccgaaacg cacgcggatt ttcacttcat tggctaaaat cgcccgatct tcatactcaa
    44941 ccagcgcagc aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa
    45001 gaaactggcc gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt
    45061 ccgaaagcgc atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc
    45121 gtccccagat gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat
    45181 tgctggcgct ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc
    45241 agtagacgaa cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa
    45301 tttgcagacg gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt
    45361 cgaagtagct tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga
    45421 tcagcgccat ctcggtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca
    45481 ccagcaaaat gccagagaac atatacaccg tgtaaaagct ggcattgatg gtcatccgac
    45541 ctttaaagcg caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca
    45601 cggatgaaac caccgacacc accagactgt tacggaagta gtcgacaaac gggaaaatca
    45661 tcgggttaaa aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat
    45721 gcagtgagat cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa
    45781 acagcgtgat gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc
    45841 gtttatttgt tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata
    45901 atgatgaaga gcaccaccga gatcgccgcc gcttttccta aatcattaaa ggcaaaggcg
    45961 gttttgtaga gatatacacc gagaatatcg accttggtcg tcagcaaata aacatccgcg
    46021 aacatgtaga acatccagat ggtgcgcagg gtcaccaccg tcgccaggac cggcataatc
    46081 gcgggcagcg tgacgatacg aaaccgttgc caggcattag cgccatccat ctctgccgct
    46141 tcgtacagcg atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg
    46201 aagtagcgcc agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac
    46261 cacagcggtg cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg
    46321 tagccgttgt tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag
    46381 gagaggatca ccagcgaacg cgcggttttg cgcagacgga actcgcggtt gaaaaacatc
    46441 gccactgcca gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc
    46501 atccacagcg agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca
    46561 acaaaggttg actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc
    46621 gacaccattg gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc
    46681 agcgcaaagg gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct
    46741 gggtttagtg ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag
    46801 ggagatcggc tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaccccg
    46861 tcacatcacc catgcgggta aagtttttat cccctactgc accaaagacc tgaatatttg
    46921 gcagttcacc gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt
    46981 tccaggtggc ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca
    47041 tcacccagtc ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt
    47101 ccgtctcttc ggtcttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg
    47161 cagagttttt ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatca
    47221 cagccggaag gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt
    47281 ctttcacttc catgatgtcg ttagaacccg gcatagtgtt ggcagcaagg tcgcggtaat
    47341 aggtcagtgc ctgcatcatc tctggtgtat caagggtgat tttgccttcg gcgttaaaga
    47401 cgttagcctg gttggataac gcaaactggg agaaggattg ttccgtcaac acgctttctg
    47461 ctgtaggcag cgcaatgccg tattttttat tcgccgggtc attcagtttc tgtgcaacgt
    47521 ccagcagctg ttgccagttt ttcggctcct caagccccgc ttttgccagc acatctttgc
    47581 gataccagat accgccaatc caggcgctga caggaacacc ggtccatgcg ctaccatctt
    47641 cggtacgcac aatacgcagt acgccatcgt aaaacgcgcc ttcaccaacg ttgctgatga
    47701 ctgtggcaac cgctttgcga tcgataagct gctctttgtc catcactttg gcgtagtcat
    47761 ggctggtttc tatcacttcc ggcagcgaac cgctacgtga aagagtaatg actttagtgt
    47821 tataggcatc ttcttccacg ggcacttgct taacggtgat gccagggttt tctttttcaa
    47881 aacgggcaat caatttactg ataacggcct ggcgctcctg ctccaccgaa gaatgcataa
    47941 attcgatgga tacattcgtt ttattttctt ctttacagcc tgaaatcagg gcgcaggaaa
    48001 ccagtgctga taacagcacg atttttgatt taatcatgag atgttccttt ttatttaatc
    48061 cacataacct gccaggggcg cagcgttatt tcattaccca atatatcaac ttcactaatc
    48121 agatcccgac catgcagatt agtaatattt acatgctgaa tatttttact gacattaaac
    48181 aatccagtca gacaattacc atcagcgtta cttctttgaa tacgcattac ggatgaatta
    48241 atcgtatcga tggtaaaatt attatccgga tgaaactcgt tatggctgcg acgaagtgta
    48301 attaaacgcg acaactcatg atataccgca tgccttaatg tagcttcatc gttcagttct
    48361 cgggttatct ctttactgtg atatttttta cggttaatcg cacggttata tccgagtttt
    48421 tcgacacctg cgtaatcatt acgcgagcca agaatacttt gaatatatat cgctggcaca
    48481 ccggggaaac ttaacaaaat cgcatgggcg agaataaacc tggcgcaacg ttcttcatcg
    48541 ctactctcac ggcggcttaa cgcatccata taggtcacat ttatttcata cggactgcgt
    48601 gtaccgtcgg gattattttt ccagtttact aatgcacctt cctgctgtaa cgcctcgacc
    48661 agctctaata tttcgctttc aggcaacaag ccccgtagcg ggtttagccc gatgccatcg
    48721 tgagaggcga ggaagttaaa ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc
    48781 cacgcacaaa gcgcctcaac gttctgtttt tgcaccgcat gcagcaccag tggcggcagc
    48841 gagaactggt acaccatatg tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct
    48901 ttatgcggaa cattggtctc ggtaatgatc actgtacctg gcgcaacgtt atcaataatc
    48961 gaccgtaaca gtttgataat cagatgtgtt ttttccagat ggatgcagct tgttcccggc
    49021 tctttccaca taaagccaac ggcatccagg cggacatact cagcaccttt cgcaaggtaa
    49081 cagagtaaaa catccaccat cgccagcaac acttcagggc tacggtaatt caggtcaatt
    49141 tgatcgtcac taaaggtggt ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc
    49201 gttaataacg gtaacgcacg cgggcgagtg acggcgctga gatcggtttg cgggtcaacg
    49261 gcaataaaaa aatcttcaaa acctggatgc tgttgtaaat agtttttaaa tcattcactt
    49321 tttgccgaca tatggttgca gacaaaatca aacattaaat gactgcattc cccgagttgc
    49381 tgaatatcct gccactcccc cgcttcactg gcgacctgat gataatcaat taccgaaaag
    49441 ccatcatcag atgaccatgg ataaaacggc aacaaatgaa catgtgaaaa aatgctttgc
    49501 agccattgat ggtaaaactg attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat
    49561 tgatcggcat aggtaattaa cacgacatca ctttcatccc agtgtttttt acgtcgctgt
    49621 gtaattaatc gtttcgcact ctcaagacgc gttacaagtt tctgtaaatg agttgcggta
    49681 aatgttccac cgtagatttc gtccaggtaa tccgtaattt tctgtttcat gttgcctttc
    49741 cgtggtagcg gtcccatgaa acgagagact actccgcaga aaataaactg tcaattgagc
    49801 acaagggttt tagtcacgca ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc
    49861 tgacgcaaat aatctatggg gatgtaaata aggtgtgaca gaaaaaaaac ggcgcataag
    49921 cgccgctcat ggtgaattct tttaaccttt gaccttcggc attgcgatgt ctttcaggcc
    49981 accggcgttc tcaacgtggg tatatcccat ctcgctaagg atctcttttg cttgccctga
    50041 ctggcgtccg gcattgcaat acactttcac ggtgtcgttt ttatccggaa cggcggtggc
    50101 aatgcgctct ttcacttcct tcaggggaat attgatggcc ccctgaacgt gctcttgctg
    50161 atactgctct ggaacacgaa catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa
    50221 caccagtgcc agagcaagta agcctttttt aaacatgtaa gcgtccttct atggatgaaa
    50281 attgccatcc attatacgcc tgagatttaa caatttttgc gaaactcccc tcacctttta
    50341 taacgctgtg ccagtttatt agcagcccga cttaacagcg gttccagcgc cacagccagc
    50401 aacattttca gcggtcggcg agcaactgat tttatcgccc agcccgctac acccgccggg
    50461 ccatagcgca gtgcggtaag aagtaccagc ttgcctgcta atttgaaacc aggctttacc
    50521 ttttgcccgg cctgttgcca gcgagtattc ataactttcc tcacagttga cggaaacggc
    50581 tacgtaacgt gaaagtatcg gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac
    50641 ttgccgccag ttcacggtcg acttcatcca gcaattcgct gctggaaggt agctgctcac
    50701 caaaggccat gttgtccggc attggatcaa gcgcaaatga caaaatgatg taagcaacca
    50761 gggtaaacag cgccagaccg aagaaaatcg acagcaccac caggatacgc accagttttg
    50821 ccggtacatc aaaatagttg gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg
    50881 gaatacgcca taatttttta ttgagattaa tgcccgccat tagcgatccc tccagttcgg
    50941 atgttctgca tcaagaatag attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc
    51001 atcagccagt tgcgctaatc gctgctgctc actttgcgac aattcactgc gaccagaacg
    51061 attgctgtaa tgcagccata accagatcgg taaaacaaac agcacaaaaa tggttaacgg
    51121 aatagccaga aatagcgcgc tcataagtac tccttagacg atgagcggcg gcgcgttcgg
    51181 acgccgcctg gatgttatta ttgattgtct tgcttcattt tggcttttaa ttgtgccagt
    51241 tgttcgctga ttgcatcatc ggctttcagt tcggcaaact gatcgtccag cgatttttgt
    51301 ttaccgaagc tgtggctttc tgcttccgct tccatctggt caatacgacg ttcgaaagat
    51361 tcgaaacgag ccattgcttc atccagtttg ccactgtcca gctgacgacg cacatcgcgc
    51421 gacgagtttg ccgcctgatg acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc
    51481 aatttgtttt ccagctcacc gatctctttc ttcatgcgtg ccagcgtgtc gtccaccagc
    51541 gtcacttcat gttccaggct cttaatcaga tcggtcagtt tctgtttttc aattaacgct
    51601 gcacgtgcca gatcctctct ctctttcagc agcgccagtt cggctttttc ctgccattca
    51661 acttcacgcg ccgacgcttg ttcaatacgg cgagtcagct gtttcttttc tgccaacgcg
    51721 cgcgccgaag tagaacgtac ttcaaccagt gtatcttcca tctcctggat catcagacga
    51781 accagtttct gtggatcttc cgctttctct aacagagcgt tgatgttggc attcacgatg
    51841 tcggcaaagc gagaaaaaat acccataatg ttgtcctctt gatttctgcg ttcaggattg
    51901 tcctgctgaa ctgttaatac aatttgcgtg ccaatttttt atctttttga tttataaagt
    51961 tctgattgaa gaatcaacag caacatgcca ggatgagtta gcgaattaca ctaacaagtg
    52021 gcgaatttca tcatggcaga atacaaagat aatttacttg gtgaggcgaa cagctttctc
    52081 gaagtgctgg aacaggtttc gcatctcgca ccgctggaca aaccggtgct catcatcggc
    52141 gaacgcggca ccggtaaaga gctgattgcc agccgcctgc attatctctc ctcccgttgg
    52201 caagggccgt ttatttccct taactgcgcg gcgttaaatg aaaatctgct ggattccgaa
    52261 ctgtttggtc acgaagcggg ggcgtttacc ggtgcgcaaa aacgtcatcc tgggagattt
    52321 gaacgtgccg acggcggtac gctatttctt gatgaactcg ctacggcacc gatgatggtg
    52381 caggagaaat tattgcgcgt gattgagtac ggtgaactgg agcgcgttgg cggcagccag
    52441 ccattgcagg tgaatgtgcg gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc
    52501 aatgaaggca cttttcgcgc tgacctgctc gaccgactgg cttttgatgt tgtacaactg
    52561 ccaccactgc gcgagcgcga aagcgacata atgttgatgg cagaacactt tgccatccag
    52621 atgtgtcggg aaattaagct gcctctgttc ccggggttta cagagcgcgc cagagaaaca
    52681 ttgctgaatt atcgttggcc gggaaatatt cgtgaattga aaaacgtggt ggaacgttca
    52741 gtgtatcgcc acggcaccag cgattatccg cttgatgaca tcattattga tccctttaaa
    52801 cggcgtccgc ctgaagacgc tatcgccgtt tcagaaacca cctcgcttcc aacactgccg
    52861 ctggatttac gtgagtttca gatgcagcag gaaaaagagt tgctgcaact cagtttgcaa
    52921 caagggaaat ataaccagaa gcgcgcggct gaattactgg ggttaactta tcatcagttc
    52981 cgcgcgttgt tgaaaaagca ccagatttag cgaagttgag caatacaact tgccggatgc
    53041 ggcgtgaatg ctttattcgg catacataat caatacgtcg tttgcaggcc tgataagcgc
    53101 agcgcatcag acattattgc gttgggctat taatcgctca gcgcatcctg caaaattttc
    53161 attgccgtat cgaattgcgc atccggtatg gtcagcggat acaggaagcg aatcacgttg
    53221 ccgtatgcgc cacaggtcag caggagcagc ccctgcgcca gcgcgcgttg ctggattttc
    53281 tgtgcaatcg ccgctgacgg ctcgcccgtt tgcggatcgt taaactctgc cgcaatcatc
    53341 gaccccaggc cgcgtaccgc agcaatggcc ggaacgcttt ctttggcatc aatcaacgtg
    53401 tttttgagac gctggcccag ttgattcgcg cgttcgcaga gtgattcttt gtcgataata
    53461 ttgagcaccg cgtgcgcggc agccaccgcc agcgggttcc cggcgtaggt gccacccaac
    53521 ccgcccggcg cgggcgcgtc cataatattc gcgttaccga ccacgcccga aagcggcatc
    53581 ccgcccgcga ggcttttcgc catcgtcatt aagtccggct tatcggcgta atgatccatg
    53641 gcaaacagtt taccggtacg cgcaaagccg ctttgcactt catcggcaat catcacaata
    53701 ccgtgctcgt cgcacaggcg gcgaatggcg gcaaccagct cttttggcgc aacgttgaaa
    53761 ccgccctcgc cctgcaccgg ttcgaaaata atggccgcga cctgcttcgc ttcgatgtct
    53821 gatttaaaca agcgttcgat ggcgtcgagg gagtcctgtg ttgaaatgcc gtgtaaatct
    53881 gacggataag gtacgtgata caccgaacca gggaacgggc cgaagccgat tttgtacggc
    53941 gcaacttttc cggtcagcgc catggtcata tatgtacgac cgtggaagcc accgctaaac
    54001 gcaatcacgc caggtcgtcc ggtatgggcg cgagcaattt tcaccgcgtt ttccaccgct
    54061 tccgcaccgg tagtgaagaa tgcggtttta gcctgcccgc tcactggggc aagggcgtta
    54121 attttctccg ccagcgtgac gtagctttcg tacggcacaa tctgatacgc cgtgtgggta
    54181 aactgttgca attgctgctc caccgccgcg accagatcag ggtggcgatg cccggtattc
    54241 agcaccgcaa tgcctgcggc gaaatcgatg tattcgttgc cctctacgtc cttcagcgta
    54301 gcgttttctg ccgactgggc gaagaagtta cacatcacgc caaccccgcg tggggtagca
    54361 gaaagacgac gctgatggaa ttcattgttg ctcatgtttc acaccttttg actcttcact
    54421 gttaatcgga aattaaacgc ccagacgatc gcgcaggctg taataagccg cgcccatcgc
    54481 ggtaaatggc acgcgcagcg tgcgcccgcc ggggaacggg taatgcggca gattggcgaa
    54541 ggcatcgaaa cgttcggcgt cgccgcgcag cagttcggca atcagacgcc cggcaagatg
    54601 ggtacaggtg acgccgtggc cgctgtagcc ctgcatgtaa tagatgttgg tatcaaggcg
    54661 accgaactgc ggcatgcgcg acaaggtcag caggaagttg tccgtccagc ggtaatcaat
    54721 tttcacgccc ttcagctgcg ggaaggtttt cagcagtttc ggcaccacaa ggcgctcaac
    54781 gtcatccggg tcgcgcgcgc cgtagaccac gccgccgccg tacagcaggc ggttgtcggc
    54841 ggtaagacgg tagtaatcaa gcagatagtt acagtcttcc acacagtagt ttttcgggat
    54901 cagcgaacgg gctaaatctt ccgacagccg ttcggtggtg atcacctgcg tgccgcacgg
    54961 catgctgcgt ttcgccagtt ccggctctac tttatcgccc agatacgcat tcccggcgac
    55021 aatcacatac ttcgccgtca cctgaccttt ggcagttcgc acaacggctg gcgtggtgtg
    55081 ctggatctgc gtcacggcag aaagttcata cacgcgcccg ccgttgaggc ggatggcgtc
    55141 cgcttcgcca atggcgaggt ttagcggatg aatatgccca ccgctgtgat ccagcagcgc
    55201 accggtatag cgatcgctgg ctacttcacg gcgaatggcg ttggcgtcca gcaattccag
    55261 ctgtttattg ccgtagcgtt cccagttctc tttctgctct tcaagtgtgg cgagctgttt
    55321 atcattcatc gccacaaaca ggccgccggg gcggtagtcg cagtcaatct gataacgttt
    55381 gatacgttcg cggatgatct caccaccttc gaacatcatg ctgccgagca tacgggcggt
    55441 gtccatgccg tagctttttt cgatcacgtc gatgtcgcgg ctgtaggagt tcacaagctg
    55501 cccgccattg cgcccgcttg cgccaaagcc gatgcgtgag gcttcgagaa ccactacgtc
    55561 aaagcccgct tccgccagat gcagcgcgga agagagcccg gtatagccgc cgccgaccac
    55621 gcaaacgtcg caggaaatcg actcattcag cgtgtcgaat ggcgcgtatt tattcgcact
    55681 ggcggcgtag taactgctgg tatgttcggt catttcaggc ctccaggctt atccagatgg
    55741 ttttcagttc ggtgaatttt tcaagggcat gcagggattt gtcgcgaccg ttgccgctct
    55801 gcttatagcc gccaaacggc acggtcatat cgccgtcgtt gtagttattg acgaagacgg
    55861 aaccagcctt caggcggcgg ctcatgcggt gcgcgcggga gaggtcgcgc gtccataccg
    55921 ccgcgccaag gccgtactgg ctgtcgttgg caagctgtag cgcctggtct tctgatgtga
    55981 aacgcgtgac caccagcacc ggaccgaaaa tctcttcgcg gcttaaggac gcattcgggt
    56041 ctacatccac aaagatggtc gggccgatgg cggcagccag ctcagcgtta cgaccatcca
    56101 gcagcagttg ccctttgctt tcgccttccc gaataaagct atggaccgag tcagcgtgag
    56161 cgctgtcgat taaggtgccc atggtggttg cgggatcaag tggatggccc ggctgccagt
    56221 tttgcgcctg ctgttttaac agggctaaga attcatcggc gatgctctct tccagtaaca
    56281 aacgcgttcc ggcgatgcac acctggccct ggttgtagaa gatgcctgcg gcggtggcgc
    56341 ttgccgcttt ttgcagatcc ggacaatcgg cgaaaacgat gttggcgctt ttgccgcccg
    56401 cttccagcca gacgcgtttc atgttgctct cgcccgcatc tttcagcagc tgtttcccgg
    56461 tacgggtcga accggtaaag gcaatggcgt cgatatcgtt atgacgcgac agcgcctgcc
    56521 ccgcttcatg accaaaacct gtcaccacgt tcaaaacacc atccggcaag cctgcttctt
    56581 tcgccagccc cgcgagacga atcgcactga gcggtgattt ttcagacggt ttcaggacaa
    56641 cgctgttccc tgctgccagc gccgggccaa gcttccagca ggtcagcagc agcggaaagt
    56701 tccacggtac gatggcggca atcacaccga ccggttcacg cacgatcatc gccagctcat
    56761 ggctactggt ggtcgccact tcgccataca ctttgtcgat cgcttcggcg taccagcgaa
    56821 tagcgcgcgc cgcgccggga atatcatcac gcagactgtg acgaatcggt ttgccggtgt
    56881 cgagagtttc cagcagtgcc agctcttcgg cgtgggcttc cattaaatcg gtgagtttat
    56941 tcagtaccgc tttacgttta gccggagaag agagtgacca gtcgccgcgt tcaaatacgc
    57001 cgcgtgctgc gctcaccgca cggtcgatat cgacgctctt gccgcgggca attttcgcca
    57061 gcggtgcctg ggtgaccgga tcaacggttt caaaggtttc attttccgcc gcagcagtat
    57121 attcaccgtt aataaataag cgggtttcaa tggcgagact taacgcttta tcctgccagt
    57181 aagccagatg atgaaaattc attatgactc ctgtttcacg tctatcagat atatgcagtt
    57241 tgtgaacgtc agggcgcaga gatccccggg ccgtatgccc gtttatttct ggcgacattc
    57301 tttatataca cttcatcctt caagccgcct ctgccttggc tgcgttcgct cccccgaatc
    57361 acttacctga gtaaactcat cgggattcgc tcacttgccg ccttgatgca acttgaatga
    57421 ttttgtgtat agattgccgg gcgcattgcg cttacccggc ttacagaaca aaaagattaa
    57481 aacgtggtgg gcgtatgggc gctgataatt cggcaaatac ctgccgacgt attactgaaa
    57541 ctgtgcggga tgccggtatt aatggcataa ctttgccccg cgacgaggtg gtaatcctga
    57601 ccattaatcg tcagaacaat ttcaccttcc agtactgtgc ctatttcctc accctgatgc
    57661 ttaattcttt ccccagtggt tgtgcccggc tggtacgttt caaagatcat cgccagcgtg
    57721 cgattcgggt taccgttatg aaccagcttc attgacacac cctgactgcc catctcaatt
    57781 aagtcgtcct gattaatgac gacctgcggc tcatcaggtt tttccggctc ggaaaagaat
    57841 tccgagagtg acagaccata caccttcagc agcttttgca gcgtactgat ggcagggctg
    57901 actttatctt gttctatcgt actgatagca ctgtgagtca gcccggagag ttcggcggca
    57961 cgacgttgtg aaagcccctg ctgctggcgg atttccgaca agcgttttcc tggcgccagt
    58021 ccctcatcac tcatatttgc atttccttaa actgtagtgg tcagagtcgc tgtttttcag
    58081 cgatatggtg ctgacaagcg gtgataaagc cctcgaacaa tatacgcgaa agcgcgtact
    58141 cgctactgtt ccattccggg tgccactgta cgcccagcgc aaaaggatga ttgatgacgc
    58201 taaccgcctc aactaagcca tccggcgagc gagcttcaac acgcaaccgt gggctaacga
    58261 ccttcgcccc ttgcccatgc agagagttta cccaaaagtt gctacattca ggtaacaacg
    58321 cagacagtaa tcccccctct tcaacctgaa cttcatgcga cggtgcatat tgctgttcca
    58381 ccggcagttc gggatcttcc cgatgttcca gcaattcagg ctgttcgcac agcttgcgat
    58441 gcaacgaacc accggttgcc accaccagtt cctgtaaacc ccggcagatg gcgaaaatgg
    58501 ggatgcgcct ttcgagtgcg gcattaatta acgccatgct cagaagatca cgcccgggat
    58561 cggcgtcagg ctcatcgccg ttttcaccat ataggtgcgg ctgcacattg ctgggactac
    58621 caggaagata aatgccatcg agtttcggca aaagttgttc aagtaatgac ggttccgcca
    58681 gcgcatgtgg tagcgcaatt ggcaagccgc ctgcgtggat gatggcattc aggtactttt
    58741 cttgcagagt ctgggtcgca tgacccttaa gcctgttcct gcacattacg acaccgataa
    58801 ccggattgtt cattatattt tccatgctca atctcacaaa gtggactaaa ttatcgccat
    58861 tactgcggtt taaatgacct ttatgttcaa tattttttca atctagcagt gggttttcat
    58921 ttttgcaaac tcaatttaac atttgacaaa catttagttt gcatacagat tcgaatggtg
    58981 gtcattatat tttacgcttt gataacgagc ggaaaacaaa ccaaaggcga agaatcatgg
    59041 aaaccaatat cgttgaagta gagaactttg ttcagcagtc agaagagagg cggggtagcg
    59101 cctttacgca ggaagtgaaa cgctacctgg agcgctaccc gaatacgcaa tatgttgatg
    59161 ttttacttac tgatttgaat ggttgcttcc gtggaaaacg tatccctgtt tcaagcctga
    59221 agaagttgga gaaagggtgt tatttcccgg cctcggtgtt tgcaatggat attctgggca
    59281 acgtagtaga agaagcgggt ctgggtcagg aaatgggcga gccggatcgt acttgtgttc
    59341 ctgttctcgg ttccttaacc ccttctgccg ccgatccaga gtttatcggt cagatgctcc
    59401 tgaccatggt cgatgaagat ggcgctccct ttgacgttga gccgcggaac gttcttaacc
    59461 gcctgtggca gcagctacgc cagcgcggat tgttcccggt tgtagcggta gagctggagt
    59521 tctatttact ggatcgtcag cgcgacgctg aagggtatct gcaaccgccc tgcgcgcccg
    59581 gcaccgatga ccgcaataca caaagccagg tttactccgt tgataacctc aaccacttcg
    59641 ccgatgtgct caatgatatt gatgaactgg cgcagttaca gttaattccc gccgacggtg
    59701 cggtcgccga agcctcgccg ggtcagtttg aaatcaacct ttaccatacc gataacgtgc
    59761 tggaagcctg cgatgatgcg ctggcgctaa aacgcctggt gcgtctgatg gcagaaaagc
    59821 ataagatgca cgccactttt atggcgaagc cgtatgaaga gcacgcgggc agcggaatgc
    59881 atatccatat cagtatgcaa aataaccgtg gcgagaacgt gctttctgac gcggaaggcg
    59941 aagattcgcc gctgctgaaa aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga
    60001 tggcgttgct ggcaccaaac gtgaactcgt atcgccgctt ccagccggga atgtatgtgc
    60061 cgacgcaggc gtcgtggggc cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg
    60121 accgtcataa tcaccgcgtg gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg
    60181 tgatggcagc gatttttgcc ggtattttgc atggccttga taacgagctg ccgttgcagg
    60241 aagaagtcga aggcaacggg ctggaacagg aaggcttacc cttcccgatt cgccagagcg
    60301 atgccctggg tgagtttatc gagaatgatc acctgcgccg ctatttaggc gaacggtttt
    60361 gccatgtgta tcacgcctgt aaaaatgatg agctgttgca gtttgagcgc ctcatcacag
    60421 aaacagaaat tgagtggatg ttgaaaaacg cgtaattccc catgcggggt tgtttcatcg
    60481 gcctgagcgg agccaggcct gacatttgct ggtaatccag acatttcccg gatgtcgcgt
    60541 tatggagcgc aggcggcaac gggcttccgt taacgattaa gactgtgacg aggaaatgag
    60601 atgatgccga tgattaaatc cccgcatggc gagggaggct gcgtatgcgc ccctcctgct
    60661 actgactgga ctccgccccc tttgcttccc ttgctaaaca ggtttgattt caggtcgaca
    60721 cgaccgcaaa ccttattacg caggggaggc agcaattatg gctattaatt caccactgaa
    60781 tattgctgcg caacccggca aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt
    60841 aatgatgggt ctggcctatc tcacgccgat gaccgtattt gatacttttg gtattgtctc
    60901 cggcattagc gacggtcacg ttccagcgtc ctatttgctg gcgctggcgg gcgtgctgtt
    60961 taccgctatc agctacggca aactggttcg ccagtttccg gaggccggtt cggcctatac
    61021 ctacgcgcaa aagtcgatta atccgcacgt cggatttatg gtcggctggt catcactgct
    61081 ggattatctc tttttgccaa tgatcaacgt tttgttggcg aaaatctatc tctccgccct
    61141 cttcccggaa gtgccgccgt gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc
    61201 gaatctgaag agcgtcaacc tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat
    61261 ctccatcatg gtggtgttta tcttcctggt ggttcaggga ctgcataaag gagaaggcgt
    61321 tggcaccgtc tggtcacttc agccgtttat cagcgagaac gcgcacctga tcccgattat
    61381 taccggtgcg acgattgtct gtttctcgtt cctcggtttc gatgcggtga ccacgctttc
    61441 ggaagagacg ccggacgccg cacgcgtgat cccgaaagcc atcttcctga cggcggtata
    61501 cggcggcgtt atctttatcg cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag
    61561 ccgctttaaa gacccggacg ccgcactgcc tgaaattgcg ctgttcgtcg gcggcaagct
    61621 ctttcagtcg attttcctct gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc
    61681 acatgccagc gtgtcgcgtc tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg
    61741 cgtgtttggc tatgtgcacc caaaatggcg gactccggca ctcaacgtca ttatggtcgg
    61801 gattgtcgcg ctgtcggcgc tgttctttga tttagtcacc gcgacagcac tgattaactt
    61861 cggtgctctg gtggcgttta ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg
    61921 taaaggaatg aataaaagct ggaaggatca cttccactat ttgctgatgc cgctggttgg
    61981 cgcgctgacg gtgggtgtgc tgtgggttaa cctagagtca acgtcactga cactcggtct
    62041 ggtatgggct tcgttaggcg gcgcatattt gtggtatctg atccgccgct atcgcaaagt
    62101 gccgctgtac gaaggtgaca gaacgccggt gagcgaaacg taatagccgg atgcgcggcg
    62161 cattatcctg cctgagggta acgcgccgcg ccattaacta taaaaagatg tatgacccgg
    62221 atcacgagtg ctatcatccg cccttatgct tagcgcatta accccagggg caaataatga
    62281 accacgatat cccgctaaaa tattttgata ttgccgatga gtacgcgacc gaatgcgcag
    62341 agcctgtcgc agaggctgaa cgcacaccgc tggcccacta cttccagtta ctgctcaccc
    62401 gtttgatgaa caatgaagag atcagcgaag aagcccaaca tgaaatggct gctgaagcgg
    62461 ggattaatcc cgtacgcatt gatgagatcg cagagttttt gaatcaatgg ggtaatgagt
    62521 aattatttaa ttgcagacga tcggtcagta tcgttgtccg gataaagcgt tcacgccgca
    62581 tccggctaac aatgacacgc actgcagcgt gctttaatgg gtttctcacg atcgtaccgc
    62641 tctgctcctc gctaagttgt tctgtgagtt atagttatag agaaatcacg ggtacgaatg
    62701 ttgaacagat ggcgacgcca cctgttcaac gaccaaccac acggccctca ttacgacccc
    62761 tgaacacaaa cggtaatggc aaatgtcatt aaatattagt aagatagcct ctttacataa
    62821 aatcccttaa tattatgcgc caggtattat cgtctctttt ggtgattgct ggacttgtga
    62881 gtggtcaggc aatcgccgcg cctgaatccc ccccgcatgc tgatatccgc gacagcggtt
    62941 ttgtctattg cgtcagcggg caagtcaaca cctttaaccc atccaaagcg agcagtgggt
    63001 taattgtcga tacccttgcc gcccagtttt atgatcgact gctggatgtc gatccctata
    63061 cctatcgcct gatgccggaa cttgccgaaa gctgggaagt actcgacaac ggcgcgacct
    63121 atcgcttcca cctgcgtcgc gatgttccgt ttcaaaaaac cgactggttt actcccactc
    63181 gtaaaatgaa tgccgacgat gtggtgttta ccttccagcg aatttttgac cgcaacaacc
    63241 cgtggcataa cgtcaacggc agcaacttcc cctacttcga cagcctgcaa tttgccgata
    63301 acgtgaaaag cgtccgcaaa ctggataatc ataccgttga gttccgactc gcccagccgg
    63361 atgcttcttt tttgtggcac ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg
    63421 cccggaagtt agagaaagaa gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg
    63481 gtccgtatca gttgtcggaa taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg
    63541 acttctggcg cggtaaaccg ttaatgccgc aggtagtggt ggatttaggc tccggcggca
    63601 ccggacgtct gtcgaaactc ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca
    63661 gccagctatc cattttgcgt gacgacccgc gcttgcgttt aacgctgcgt cctgggatga
    63721 acgtcgccta tctggcattt aacaccgcca aaccgccgct aaataatccc gctgtccgcc
    63781 atgcactggc actggcgatt aataaccagc gcctgatgca atccatctat tatggtacgg
    63841 ctgaaacggc ggcctctatt ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa
    63901 ttactgaata caatccggcg aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact
    63961 taacgctgaa actgtgggtg cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa
    64021 ctgccgaact gattcaggcg gatatggcgc aggttggcgt aaaagtggtg attgtgccgg
    64081 tagaaggtcg ctttcaggag gcgcggttga tggatatgag ccatgatctg acgttatccg
    64141 gttgggcgac ggacagtaac gacccggaca gtttcttccg tcctttactg agctgcgcgg
    64201 caattcattc acagaccaac ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc
    64261 gtaaggcgct ctcctcgcag cagctggcgg cgcgtattga agcctatgac gaagcgcaga
    64321 gtattctggc gcaggaattg cccattttgc cgctggcgtc gtcattgcgt ttgcaggcct
    64381 atcggtacga tatcaaaggt ctggtactta gcccgtttgg taacgcctcc tttgctgggg
    64441 tgtatcgcaa gaaacaggat gaggtgaaaa aaccatgatt atcttcacct tacgccgcat
    64501 tttgttattg attgtcacct tgttcctgct gaccttcgtt ggcttcagct tgagctattt
    64561 cacccctcat gcgccgttac aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa
    64621 cggcctgatc cactgggatt ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca
    64681 gttaaaagag gtcttcccgg cgacgatgga actgtgcatc ctcgccttcg gttttgcgct
    64741 gattgttggc atccccgtgg ggatgattgc cgggattacg cgccataagt ggcaagataa
    64801 cttgatcaac gccatcgcct tactgggctt ttcaatcccg gtgttctggc tggcgcttct
    64861 gttgacgctg ttttgttcac tcacgctagg ttggttgccc gtttcagggc gtttcgatct
    64921 gctctacgaa gtgaaaccga ttaccggttt tgcgttgatt gatgcctggc tttcggactc
    64981 accatggcgg gatgaaatga tcatgagcgc aatccgccat atgatattgc ccgtgatcac
    65041 cctgtcggtt gcgccaacaa ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt
    65101 gtatgaccag aactatgtaa aagcggcggc gacccgcggt ttgtcgcgct ttactatttt
    65161 gcgtcgccac gttctgcata acgcgttacc gccggttatt cctcgcctgg gcttacagtt
    65221 ttccaccatg ctgacgctgg cgatgatcac cgaaatggtt tttagctggc cgggtttagg
    65281 gcgctggtta attaacgcca tccgccagca ggattacgca gccatttccg ccggagtgat
    65341 ggtgtgtggc tcactggtca ttattgttaa cgtgatttct gatattttgg gtgccatggc
    65401 taaccctctg aaacataagg aatggtatgc cttacgatag cgtatacagc gaaaagcgcc
    65461 cgccgggcac gctgcgtacc gcctggcgca aattttatag tgacgcctct gcgatggtcg
    65521 gcctgtacgg ctgcgcgggg ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt
    65581 acggcatcga ccagcaattt ctcggttatc aattattgcc gccgtcatgg tcgcgctatg
    65641 gcgaagtttc tttcttcctg gggactgacg acctcggacg cgatgtttta agccgtttac
    65701 tgagcggagc tgcgccaacc gtgggtggcg catttgtggt gacgcttgcc gcgacgatct
    65761 gtggcctggt actgggcacc ttcgccgggg cgacacacgg cctccgctca gcggtgctta
    65821 accatattct ggatacctta ctggcgatcc cttcgctgct gttggcaatt atcgttgtag
    65881 cgtttgccgg accgagtttg tcccacgcta tgtttgctgt ctggctggca ttgctgccgc
    65941 gtatggtacg ttcgatttac agcatggtgc atgacgaact ggaaaaagag tacgttatcg
    66001 ccgcccgtct ggatggcgca tcaacgctga atattctctg gtttgctgtg atgccaaaca
    66061 tcaccgccgg gctggtcacc gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg
    66121 ccgcgctggg ctttctcgat ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc
    66181 tcggtgatgc gctggaactg atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg
    66241 caattatgat tagcgtgttg ttggttaacc tccttggcga tggcgttcgt cgtgcgatta
    66301 ttgcgggggt ggaataatgc cattactcga tattcgtaac ctgaccattg aatttaaaac
    66361 cggtgatgag tgggttaaag ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat
    66421 ccgcggtctt gttggtgaat ccggttccgg caagagtttg attgcgaaag cgatttgtgg
    66481 ggtgaataaa gataactggc gtgttactgc tgaccgtatg cgttttgatg atatcgattt
    66541 gctgcgtctc tccgcacgcg aacggcgcaa actggttggc cataacgtgt cgatgatttt
    66601 ccaggaaccg cagtcgtgtc ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa
    66661 catcccagcc tggacctata aaggccgttg gtggcagcgt tttggctggc gcaaacgccg
    66721 tgccattgaa ctgctgcacc gcgtggggat taaagatcac aaagatgcga tgcgcagttt
    66781 tccctatgag ttgaccgaag gtgaatgtca gaaagtgatg atagccattg cactggcgaa
    66841 tcaaccgcgt ctgctgattg ctgacgaacc gaccaactca atggagccaa caacccaggc
    66901 gcaaatcttt cgcctgctga cgcgtctcaa ccaaaacagc aataccacta ttttgcttat
    66961 cagccatgac ttacaaatgc ttagccaatg ggcggataaa attaacgtgc tttactgcgg
    67021 tcaaacagtg gaaaccgcgc cgagtaagga gttggtgacg atgccacatc atccttatac
    67081 ccaggcgctg atccgcgcga taccagactt cggcagcgcg atgccgcata aaagtcgcct
    67141 caatacgctg cccggcgcta tcccactgct ggaacagtta ccgattggtt gtcgtctggg
    67201 gccacgttgc ccgtatgcac aacgagaatg cattgtgacg ccacgtttga cgggggcgaa
    67261 aaatcatctc tatgcctgtc atttcccgct gaacatggag aaagagtgag atgatcgaaa
    67321 cgctgcttga agtgcgtaat ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc
    67381 gtcagaccgt agaagcggta aaacccttga gctttacgct acgtgaaggc cagacactgg
    67441 cgattattgg cgagaatggt tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga
    67501 tagagcctac cagcggcgag ttattgattg acgatcatcc actgcatttt ggcgattatt
    67561 ccttccgcag tcagcgtatt cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc
    67621 gtcaacgtat ctcgcaaatt ctggattttc cactgcgcct gaacaccgac ctggaaccgg
    67681 aacagcgtcg caaacagatt attgaaacga tgcgtatggt tgggctgcta ccggatcacg
    67741 tcagttacta tccgcatatg ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg
    67801 cgctgatatt gcgcccaaaa gtcattattg ccgatgaagc cctcgcttca ctggatatgt
    67861 cgatgcgttc gcagttgatt aatctgatgc tggagttaca ggaaaaacag ggcatttcgt
    67921 atatttatgt tacccagcat atcggaatga tgaagcacat tagcgaccag gtgctggtga
    67981 tgcatcaggg cgaggttgtc gaacgaggca gcaccgcgga tgtgctggca tcgccgctgc
    68041 atgaactcac caaacggctg attgccgggc actttggcga agcattgaca gcggatgcgt
    68101 ggcgtaaaga tcgctaattt tctgaatcct ccttccagat tatccacctc attctgacaa
    68161 gagattaaat cttgtcagaa tgaggtgatg gataaaataa aatcaaatgc gcgtgattta
    68221 agacgcaatc tcactttgca ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt
    68281 ggtgatttca aatttcgccg tcaacatccg gtggggagct acattctcga ttttgcttgc
    68341 tgctcggcgc gcgtagtcgt tgagctggat ggtgggcagc atgatttggc agttgcctat
    68401 gataccaggc gcactagctg gcttgagtcg cagagctgga ccgtgctgcg tttctggcat
    68461 aacgagattg attgtaatga ggaggcggtg ctggagatta ttctgcagga actgaaccgc
    68521 cggtcaccct caccctgaaa gggcgagggg gccgaccgag ccgaatagct gttgtggtga
    68581 aaacatggag acagtgctgg agaatattcg gcaaggtctg aaccatccca gccatcgcca
    68641 taaaagggtt aggggctgta tgagcctgtt tgttgctggg gtaacaatat ttgcacaata
    68701 tggtcccctc gcccctctgg ggagagggtt agggtaaggg gaaaagcgcc caccctgccg
    68761 cagcctgctc cggtcggacc tggcaactat agctactcac agccaggttg attataataa
    68821 ccgtttatct gttcgtactg tttactaaaa cgacgaatcg cctgattttc aggcacaaca
    68881 agcatcaaca ataaggatta aagctatggg ttttctttcc ggtaagcgca ttctggtaac
    68941 cggtgttgcc agcaaactat ccatcgccta cggtatcgct caggcgatgc accgcgaagg
    69001 agctgaactg gcattcacct accagaacga caaactgaaa ggccgcgtag aagaatttgc
    69061 cgctcaattg ggttctgaca tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga
    69121 caccatgttc gctgaactgg ggaaagtttg gccgaaattt gacggtttcg tacactctat
    69181 tggttttgca cctggcgatc agctggatgg tgactatgtt aacgccgtta cccgtgaagg
    69241 cttcaaaatt gcccacgaca tcagctccta cagcttcgtt gcaatggcaa aagcttgccg
    69301 ctccatgctg aatccgggtt ctgccctgct gaccctttcc taccttggcg ctgagcgcgc
    69361 tatcccgaac tacaacgtta tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta
    69421 tatggcgaac gcgatgggtc cggaaggtgt gcgtgttaac gccatctctg ctggtccgat
    69481 ccgtactctg gcggcctccg gtatcaaaga cttccgcaaa atgctggctc attgcgaagc
    69541 cgttaccccg attcgccgta ccgttactat tgaagatgtg ggtaactccg cggcattcct
    69601 gtgctccgat ctctctgccg gtatctccgg tgaagtggtc cacgttgacg gcggtttcag
    69661 catcgctgca atgaacgaac tcgaattgaa ataatcattc tgttggtaaa tattgggcgg
    69721 cgttctgccg cccgttatct ctgttatacc tttctgatat ttgttatcgc cgatccgtct
    69781 ttctcccctt cccgccttgc gtcaggataa cgatttcctt tacgaccaag gagcgcccat
    69841 ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc
    69901 ggagtatgac gttctgcctc tggtcccgga agccgcaaag gtcagcgatc cctttctgct
    69961 cgacgtgatc cttgatgaag aaacgctggc tcccttcctt tcatggctgg tccctgcgcg
    70021 cgttcttgca gtggaattgt tccctgacca gcttaccgtg acccgttcac agaccttcac
    70081 cgcttatgaa cgcttgtcta cggccctgac ggttgctcag gtttgcggcg tccagcggtt
    70141 atgtaactac tattcagcgc gacttacgcc gctccccggg cctgattcct ccagggaaag
    70201 caatcatagg ttggcacaaa tcacgcaata tgcccgccaa ctggctagct cgccttctat
    70261 tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt cttactgccg gggactgtgt
    70321 gatcattaac caaatcattg gttttattgg ctttcaggcg cggaccattg cgacatttca
    70381 ggcttatctc gggcacccgg tacgctggtt acccggtctg gaaatacaaa actacgccga
    70441 cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc agctatgagg tggaaaaact
    70501 acctgaagag tacacaaaaa gttcaacagc agaactttgc caactggccg aaacactctc
    70561 tctccaccct atttcacttt cccttctcga aaggttgtta aacagtacac gggttaatac
    70621 acagccggat aataagcttg cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg
    70681 ttttgccgcc tgtatggatt catcaaatga atataaaaaa atcagccccc ttctgcgcaa
    70741 gggcgaaaat gaaattaacc aatgggctga ccgtcattct gttgagcacg ctaccgttca
    70801 ggcgatacaa tggctgaccc gagcacccga tcgctttagc gccgcccagt tcagcccttt
    70861 actcgaacac gaaaaatcat caacgcagat tattaatctg ctggtatgga gcgggctgtg
    70921 tggctggata aatcgcttaa aaatcgcgtt gggtgagaca tattaacctt gccgcgtcag
    70981 acagattcgc gtaaaactgt cagccgctct aatggccacc aaaatagaca attatgtttc
    71041 aggacaaccc gctgctagcg cagcttaaac agcaactgca ttcccagacg ccacgcgctg
    71101 aaggggtggt aaaagccaca gaaaaaggct ttggcttcct ggaagtcgac gcgcaaaaaa
    71161 gttatttcat tccgccgccg cagatgaaaa aagtcatgca tggcgaccga attatcgcgg
    71221 tgatccacag tgaaaaagaa cgtgaatccg cagagccaga agaactggtt gaaccgttcc
    71281 tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg tctggccatc gttcctgatc
    71341 atccactctt aaaagacgcc attccttgcc gcgcagcccg tggcctgaac cacgagttta
    71401 aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc gctgaaaggc gatcgttctt
    71461 tctatgctga actgacacaa tacatcactt ttggtgacga ccactttgta ccgtggtggg
    71521 ttacccttgc gcgccataat ctggaaaaag aagcaccaga cggcgtcgct accgaaatgc
    71581 tcgatgaagg tctggttcgt gaagatctga ccgcgctgga ttttgtcacc atcgacagtg
    71641 ccagcacaga agatatggat gacgcccttt tcgctaaggc gttgccggat gacaaacttc
    71701 agctgattgt ggcgattgcc gatccaaccg cgtggattgc tgaaggcagc aagctggaca
    71761 aagccgcgaa aattcgcgca ttcaccaact atctgcctgg cttcaacatc cctatgctgc
    71821 ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa tgaagtccgc ccggtactgg
    71881 catgccgcat gacgctctcc gctgatggca ccattgaaga taatatcgaa ttctttgccg
    71941 ccaccatcga atccaaagca aagctggtgt atgaccaggt ttctgactgg ctggaaaata
    72001 ccggtgactg gcagcctgaa agtgaagcaa ttgccgaaca agtccgtttg ctagcgcaaa
    72061 tttgccaacg ccgcggcgag tggcgtcata accacgcact ggtgtttaaa gatcgcccgg
    72121 attaccgctt tattctcggt gaaaaaggtg aagtgctgga tatcgtcgcc gagcctcgtc
    72181 gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc taacatttgt gcggcccgcg
    72241 tactgcgcga taagctcggt tttggcatct ataacgtgca tatgggcttt gatccggcga
    72301 atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct gcatgtcgat gccgaagaag
    72361 tgctcacgct ggacggtttc tgcaaactgc gtcgtgaact ggacgcgcaa ccaactggtt
    72421 tcctcgacag ccgcattcgt cgcttccagt catttgctga aattagcact gaacccggtc
    72481 ctcactttgg cctcggtctg gaagcatacg ccacctggac ttcgccgatc cgtaaatatg
    72541 gcgacatgat caaccaccgt ctgctgaaag cggttatcaa aggcgaaact gcgacgcgcc
    72601 cacaggatga aatcactgtc caaatggccg agcgtcgccg tctcaaccgg atggcagaac
    72661 gtgatgttgg tgactggtta tacgcacgct tcctgaaaga caaagccggg accgacaccc
    72721 gtttcgcagc ggaaattgtc gatatcagcc gtggcggcat gcgtgttcgt ttggttgata
    72781 acggcgctat cgcctttatt ccggcacctt tcttacacgc tatgcgcgat gaactggttt
    72841 gcagccagga aaacggcact gtacaaatta aaggtgaaac ggtttacaaa gtgactgacg
    72901 ttattgacgt cactattgcc gaagtccgca tggaaacccg cagcattatt gcgcgcccgg
    72961 tcgcgtaatc tcctttcacg gcccattcct catggatggg ccgtttattt ccccgctcta
    73021 ccttcgttat atccaggcag attttttaac tgactttcgt ttgaaaactg gcgtttttcc
    73081 aaaactggat tacaaataaa tatataaaaa aaacaatgcg tttagccgcc gttaatttca
    73141 tgtaagtcat gttgttcctt ttatttaacc gcggactccg cagttaaccg gaggatatgc
    73201 atcatgaaaa ccgttaggga gtccacaacg ttgtacaact ttctcggatc gcacaatcca
    73261 tactggcggt tgacggaaag cagcgatgtt ttgcgctttt ctaccaccga aaccacagaa
    73321 cctgatcgta cattgcagtt atctgcc
//
LOCUS       DAZGTR010000009        67413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGTR010000009 DAZGTR010000000
VERSION     DAZGTR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.009"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..883
                     /locus_tag="ACHLCY_000695"
     CDS             <1..883
                     /locus_tag="ACHLCY_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFP0368011.1"
                     /translation="PMPRHANPEQLEKTVRYLTQTVHPRSADNIDNLNRSAEYIKEVF
                     VSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGARYDSASSYENDELTYTPGAD
                     DNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSAVHAASLERPVKL
                     MIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVRQVKATLLSSR
                     YLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQYHLPGDTADRLNYQ
                     KMAQVVDGVINLLYNSK"
     gene            986..1489
                     /locus_tag="ACHLCY_000696"
     CDS             986..1489
                     /locus_tag="ACHLCY_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP0368012.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1556..3013)
                     /gene="nuoN"
                     /locus_tag="ACHLCY_000697"
     CDS             complement(1556..3013)
                     /gene="nuoN"
                     /locus_tag="ACHLCY_000697"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP0368013.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3020..4549)
                     /gene="nuoM"
                     /locus_tag="ACHLCY_000698"
     CDS             complement(3020..4549)
                     /gene="nuoM"
                     /locus_tag="ACHLCY_000698"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP0368014.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4713..6554)
                     /gene="nuoL"
                     /locus_tag="ACHLCY_000699"
     CDS             complement(4713..6554)
                     /gene="nuoL"
                     /locus_tag="ACHLCY_000699"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP0368015.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLVT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6551..6853)
                     /gene="nuoK"
                     /locus_tag="ACHLCY_000700"
     CDS             complement(6551..6853)
                     /gene="nuoK"
                     /locus_tag="ACHLCY_000700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP0368016.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6850..7404)
                     /gene="nuoJ"
                     /locus_tag="ACHLCY_000701"
     CDS             complement(6850..7404)
                     /gene="nuoJ"
                     /locus_tag="ACHLCY_000701"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP0368017.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7416..7958)
                     /gene="nuoI"
                     /locus_tag="ACHLCY_000702"
     CDS             complement(7416..7958)
                     /gene="nuoI"
                     /locus_tag="ACHLCY_000702"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP0368018.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7973..8950)
                     /gene="nuoH"
                     /locus_tag="ACHLCY_000703"
     CDS             complement(7973..8950)
                     /gene="nuoH"
                     /locus_tag="ACHLCY_000703"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP0368019.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8947..11673)
                     /gene="nuoG"
                     /locus_tag="ACHLCY_000704"
     CDS             complement(8947..11673)
                     /gene="nuoG"
                     /locus_tag="ACHLCY_000704"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP0368020.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGIYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGA
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11726..13063)
                     /gene="nuoF"
                     /locus_tag="ACHLCY_000705"
     CDS             complement(11726..13063)
                     /gene="nuoF"
                     /locus_tag="ACHLCY_000705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP0368021.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13060..13560)
                     /gene="nuoE"
                     /locus_tag="ACHLCY_000706"
     CDS             complement(13060..13560)
                     /gene="nuoE"
                     /locus_tag="ACHLCY_000706"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP0368022.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13563..15365)
                     /gene="nuoC"
                     /locus_tag="ACHLCY_000707"
     CDS             complement(13563..15365)
                     /gene="nuoC"
                     /locus_tag="ACHLCY_000707"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP0368023.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15459..16121)
                     /gene="nuoB"
                     /locus_tag="ACHLCY_000708"
     CDS             complement(15459..16121)
                     /gene="nuoB"
                     /locus_tag="ACHLCY_000708"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP0368024.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16137..16580)
                     /gene="nuoA"
                     /locus_tag="ACHLCY_000709"
     CDS             complement(16137..16580)
                     /gene="nuoA"
                     /locus_tag="ACHLCY_000709"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP0368025.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSARFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17211..18149)
                     /gene="lrhA"
                     /locus_tag="ACHLCY_000710"
     CDS             complement(17211..18149)
                     /gene="lrhA"
                     /locus_tag="ACHLCY_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP0368026.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19069..20286
                     /gene="alaA"
                     /locus_tag="ACHLCY_000711"
     CDS             19069..20286
                     /gene="alaA"
                     /locus_tag="ACHLCY_000711"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP0368027.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20370..20969
                     /gene="yfbR"
                     /locus_tag="ACHLCY_000712"
     CDS             20370..20969
                     /gene="yfbR"
                     /locus_tag="ACHLCY_000712"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0368028.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21028..22860)
                     /locus_tag="ACHLCY_000713"
     CDS             complement(21028..22860)
                     /locus_tag="ACHLCY_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0368029.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22947..23597)
                     /gene="hxpA"
                     /locus_tag="ACHLCY_000714"
     CDS             complement(22947..23597)
                     /gene="hxpA"
                     /locus_tag="ACHLCY_000714"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP0368030.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23608..24102)
                     /locus_tag="ACHLCY_000715"
     CDS             complement(23608..24102)
                     /locus_tag="ACHLCY_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP0368031.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24185..24640)
                     /gene="yfbV"
                     /locus_tag="ACHLCY_000716"
     CDS             complement(24185..24640)
                     /gene="yfbV"
                     /locus_tag="ACHLCY_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP0368032.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24978..26180
                     /gene="ackA"
                     /locus_tag="ACHLCY_000717"
     CDS             24978..26180
                     /gene="ackA"
                     /locus_tag="ACHLCY_000717"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP0368033.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26255..28399
                     /gene="pta"
                     /locus_tag="ACHLCY_000718"
     CDS             26255..28399
                     /gene="pta"
                     /locus_tag="ACHLCY_000718"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0368034.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPS
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGCSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28589..30109
                     /gene="yfcC"
                     /locus_tag="ACHLCY_000719"
     CDS             28589..30109
                     /gene="yfcC"
                     /locus_tag="ACHLCY_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP0368035.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30142..30684)
                     /gene="yfcD"
                     /locus_tag="ACHLCY_000720"
     CDS             complement(30142..30684)
                     /gene="yfcD"
                     /locus_tag="ACHLCY_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP0368036.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30742..31293)
                     /gene="yfcE"
                     /locus_tag="ACHLCY_000721"
     CDS             complement(30742..31293)
                     /gene="yfcE"
                     /locus_tag="ACHLCY_000721"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP0368037.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31349..31993)
                     /gene="yfcF"
                     /locus_tag="ACHLCY_000722"
     CDS             complement(31349..31993)
                     /gene="yfcF"
                     /locus_tag="ACHLCY_000722"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP0368038.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32129..32776
                     /gene="yfcG"
                     /locus_tag="ACHLCY_000723"
     CDS             32129..32776
                     /gene="yfcG"
                     /locus_tag="ACHLCY_000723"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP0368039.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32833..33195
                     /gene="folX"
                     /locus_tag="ACHLCY_000724"
     CDS             32833..33195
                     /gene="folX"
                     /locus_tag="ACHLCY_000724"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP0368040.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33216..34109
                     /locus_tag="ACHLCY_000725"
     CDS             33216..34109
                     /locus_tag="ACHLCY_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP0368041.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34157..35047)
                     /gene="rpnB"
                     /locus_tag="ACHLCY_000726"
     CDS             complement(34157..35047)
                     /gene="rpnB"
                     /locus_tag="ACHLCY_000726"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP0368042.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35244..36017)
                     /gene="hisP"
                     /locus_tag="ACHLCY_000727"
     CDS             complement(35244..36017)
                     /gene="hisP"
                     /locus_tag="ACHLCY_000727"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP0368043.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36025..36741)
                     /locus_tag="ACHLCY_000728"
     CDS             complement(36025..36741)
                     /locus_tag="ACHLCY_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0368044.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36738..37424)
                     /gene="hisQ"
                     /locus_tag="ACHLCY_000729"
     CDS             complement(36738..37424)
                     /gene="hisQ"
                     /locus_tag="ACHLCY_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP0368045.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37514..38296)
                     /gene="hisJ"
                     /locus_tag="ACHLCY_000730"
     CDS             complement(37514..38296)
                     /gene="hisJ"
                     /locus_tag="ACHLCY_000730"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP0368046.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38517..39299)
                     /gene="argT"
                     /locus_tag="ACHLCY_000731"
     CDS             complement(38517..39299)
                     /gene="argT"
                     /locus_tag="ACHLCY_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP0368047.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39565..40134)
                     /gene="ubiX"
                     /locus_tag="ACHLCY_000732"
     CDS             complement(39565..40134)
                     /gene="ubiX"
                     /locus_tag="ACHLCY_000732"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP0368048.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIKTHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40229..41746)
                     /gene="purF"
                     /locus_tag="ACHLCY_000733"
     CDS             complement(40229..41746)
                     /gene="purF"
                     /locus_tag="ACHLCY_000733"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP0368049.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41783..42271)
                     /gene="cvpA"
                     /locus_tag="ACHLCY_000734"
     CDS             complement(41783..42271)
                     /gene="cvpA"
                     /locus_tag="ACHLCY_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP0368050.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42530..43192)
                     /gene="dedD"
                     /locus_tag="ACHLCY_000735"
     CDS             complement(42530..43192)
                     /gene="dedD"
                     /locus_tag="ACHLCY_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP0368051.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43182..44450)
                     /gene="folC"
                     /locus_tag="ACHLCY_000736"
     CDS             complement(43182..44450)
                     /gene="folC"
                     /locus_tag="ACHLCY_000736"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP0368052.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44520..45434)
                     /gene="accD"
                     /locus_tag="ACHLCY_000737"
     CDS             complement(44520..45434)
                     /gene="accD"
                     /locus_tag="ACHLCY_000737"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP0368053.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45590..46249)
                     /locus_tag="ACHLCY_000738"
     CDS             complement(45590..46249)
                     /locus_tag="ACHLCY_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0368054.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46332..47144)
                     /gene="truA"
                     /locus_tag="ACHLCY_000739"
     CDS             complement(46332..47144)
                     /gene="truA"
                     /locus_tag="ACHLCY_000739"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP0368055.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVMRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47144..48157)
                     /locus_tag="ACHLCY_000740"
     CDS             complement(47144..48157)
                     /locus_tag="ACHLCY_000740"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0368056.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48223..49359)
                     /gene="pdxB"
                     /locus_tag="ACHLCY_000741"
     CDS             complement(48223..49359)
                     /gene="pdxB"
                     /locus_tag="ACHLCY_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP0368057.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49458..50453
                     /gene="flk"
                     /locus_tag="ACHLCY_000742"
     CDS             49458..50453
                     /gene="flk"
                     /locus_tag="ACHLCY_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP0368058.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50450..51628)
                     /locus_tag="ACHLCY_000743"
     CDS             complement(50450..51628)
                     /locus_tag="ACHLCY_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0368059.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51912..53132)
                     /gene="fabB"
                     /locus_tag="ACHLCY_000744"
     CDS             complement(51912..53132)
                     /gene="fabB"
                     /locus_tag="ACHLCY_000744"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP0368060.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53291..55297
                     /gene="mnmC"
                     /locus_tag="ACHLCY_000745"
     CDS             53291..55297
                     /gene="mnmC"
                     /locus_tag="ACHLCY_000745"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP0368061.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVGVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55418..55696)
                     /locus_tag="ACHLCY_000746"
     CDS             complement(55418..55696)
                     /locus_tag="ACHLCY_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP0368062.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55730..56278)
                     /gene="epmC"
                     /locus_tag="ACHLCY_000747"
     CDS             complement(55730..56278)
                     /gene="epmC"
                     /locus_tag="ACHLCY_000747"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP0368063.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56278..57087)
                     /locus_tag="ACHLCY_000748"
     CDS             complement(56278..57087)
                     /locus_tag="ACHLCY_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP0368064.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(57087..57911)
                     /gene="mepA"
                     /locus_tag="ACHLCY_000749"
     CDS             complement(57087..57911)
                     /gene="mepA"
                     /locus_tag="ACHLCY_000749"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP0368065.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSSLWKPEI
                     FSLIKLAAQDKNVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57915..59000)
                     /gene="aroC"
                     /locus_tag="ACHLCY_000750"
     CDS             complement(57915..59000)
                     /gene="aroC"
                     /locus_tag="ACHLCY_000750"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP0368066.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59035..59967)
                     /gene="prmB"
                     /locus_tag="ACHLCY_000751"
     CDS             complement(59035..59967)
                     /gene="prmB"
                     /locus_tag="ACHLCY_000751"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0368067.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            60133..60684
                     /gene="smrB"
                     /locus_tag="ACHLCY_000752"
     CDS             60133..60684
                     /gene="smrB"
                     /locus_tag="ACHLCY_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP0368068.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60757..61608)
                     /locus_tag="ACHLCY_000753"
     CDS             complement(60757..61608)
                     /locus_tag="ACHLCY_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP0368069.1"
                     /translation="MHKIRHVICLLVLALCASGIQAATKIATLYVPAGTTSVVAKYRF
                     HLSVLTPQSVEYGTYESNSTAAASLPLVSWTGSPPGPELRMERNNTTLPDSTCPGLEE
                     YDALSPVTAWSCNELVLGVYYDGDLHGCPWIVSSYVESASTMDQRFGAGIFIGPTKVS
                     SDCPTESLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCDGSNFDER
                     GAYCRFVAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCRF
                     TYILNMY"
     gene            complement(61610..62149)
                     /locus_tag="ACHLCY_000754"
     CDS             complement(61610..62149)
                     /locus_tag="ACHLCY_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0368070.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGARAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVAGQSAHLPVVFKLKDCKGPAGYNVKVTLTGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(62146..62634)
                     /locus_tag="ACHLCY_000755"
     CDS             complement(62146..62634)
                     /locus_tag="ACHLCY_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0368071.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTNFSAYATLRVD
                     YQ"
     gene            complement(62631..63140)
                     /locus_tag="ACHLCY_000756"
     CDS             complement(62631..63140)
                     /locus_tag="ACHLCY_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0368072.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(63156..63908)
                     /locus_tag="ACHLCY_000757"
     CDS             complement(63156..63908)
                     /locus_tag="ACHLCY_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0368073.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(63928..66573)
                     /gene="yfcU"
                     /locus_tag="ACHLCY_000758"
     CDS             complement(63928..66573)
                     /gene="yfcU"
                     /locus_tag="ACHLCY_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFP0368074.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(66655..67218)
                     /locus_tag="ACHLCY_000759"
     CDS             complement(66655..67218)
                     /locus_tag="ACHLCY_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0368075.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16599 a        18363 c        16974 g        15477 t
ORIGIN      
        1 gccaatgccc cgacacgcaa atcctgagca attagaaaaa acagtgcgtt atcttacaca
       61 aactgtgcat ccacgtagtg ccgacaatat tgataatctg aataggtccg cagagtatat
      121 aaaagaggtc ttcgtcagta gcggtgccag aattacctcg caggacgtcc ccattacggg
      181 tggcccctac aaaaacattg ttgctgatta tggtcctgcc gatggaccgc tgattattat
      241 tggtgcgcgc tatgactctg ccagcagtta tgaaaacgat gaattgacct atacacctgg
      301 cgcggatgat aacgccagcg gcgtggcagg attactcgaa ctggcacgtt tgttacatca
      361 gcaagtaccg aaaacaggcg tgcagctggt cgcctatgcg tcggaagaac cgcccttctt
      421 tcgtagcgat gaaatgggga gcgcggtaca tgcagcttcg cttgagcgtc cagtaaaatt
      481 aatgatagca ctggagatga ttggctatta cgactctgcg cctggtagcc aggattaccc
      541 ttacccggca atgtcctggc tttatcccga tcggggagac tttattgccg tggtcggcag
      601 aatgcaggat atcaacgccg ttcgtcaggt aaaagcgaca ttgttatcat cccggtattt
      661 atctgtttat tctatgaatg ccccagggtt tattcccggt attgatttct ctgatcacct
      721 taattattgg caacacgata ttcccgccgt aatgattact gacaccgctt tttatcgtaa
      781 taaacaatac cacttgcccg gtgataccgc agacagattg aattatcaga aaatggctca
      841 ggtggtggat ggtgttataa atttgttata caacagtaaa taataaaaat ttctcgaggc
      901 gggtccagaa aaagcgacat gcacatttta ttttccctcg tctgttttaa gatgcgaaac
      961 atgaaattca atggaggaaa aataaatggg aatgattggt tattttgcag aaattgactc
     1021 agaaaagatt aaccaactgc ttgagtctac tgaaaaacct ttgatggata atatacatga
     1081 cacgctttca ggattacgcc gactggatat tgataaacgc tgggattttt tacactttgg
     1141 gctgacaggc acctctgctt ttgatcccgc gaagaatgat ccgctgagtc gtgccgtgct
     1201 tggtgaacac agcctcgaag atggcataga tggcttcctg ggattgacct ggaatcagga
     1261 gttagccgcg acaatcgacc gactggaatc actggatcga agtgagctgc gtaaacagtt
     1321 ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt
     1381 agaggggcaa ctctttgctt cgatcatgct tgatatggaa aagctgatta gcgcgtatcg
     1441 acgtatgctg cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcaggg
     1501 caccaaaaaa aaccgccgaa catgtcggcg gctttctgac ttacaaacta acagattaca
     1561 tcagcggcat tgctaaccgc acaatgctaa tcagcggttg tggccataca cccagcacca
     1621 gtaccaacag tgcagaaatc agcaccacga taccgcccgc gctgtactgc cagtttgatg
     1681 gtgcatcgcg acccggttgt tccggggcat gaagatacag gctcaccgcc acgcgcaggt
     1741 agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg
     1801 cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac
     1861 cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca
     1921 gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc
     1981 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg
     2041 cttccatcga catctcacca gtttgcagcg caatcagcgc caccagcaga tagccgaggt
     2101 gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcagc gccatcaggt
     2161 taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt
     2221 cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
     2281 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
     2341 ggtggaacgg caccagcgag agtttgaagc cgaggccaac aatcatcagt ccgaaacctg
     2401 ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg
     2461 acaggtcgcc agactgtgca tacaccagcg ccataccaaa cagcaggaaa gaagacgctg
     2521 cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt
     2581 aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac aaagacgcca
     2641 gatggttggc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
     2701 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
     2761 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
     2821 gcgtaacgtc catagcgccc gcctggccga caaaccagag cgaaaccagc gccgcgttaa
     2881 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
     2941 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
     3001 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
     3061 ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca
     3121 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
     3181 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca
     3241 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa
     3301 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
     3361 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
     3421 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa
     3481 taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg
     3541 ccaactggct gccggtgtag atagcaatca gcacgaagcc catgtgggaa accgaggtgt
     3601 aggcgatcag acgtttgata tcggtctggg cgaaggccat ccacgcaccg tagaagatgc
     3661 cgataacacc cagccacata gcaattggcg cgaactctgc cgacgcgttc gggaacagcg
     3721 gcagggagaa acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa
     3781 cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca
     3841 ccggcatttt gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac
     3901 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
     3961 agtgaacaaa aaccagcgcc aggatggcaa tcaacatcac caggccactc gcctgggtgt
     4021 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
     4081 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
     4141 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
     4201 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca tagtaccgcc agcacaccga
     4261 gcagaccggt cagcacgacc atcagcagcg acagcccgtc aatggcgaga tgaatagaga
     4321 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
     4381 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
     4441 ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc aaagcgttcg gtctgccagc
     4501 acaggaagcc gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
     4561 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
     4621 cttcggactg ggggtagtgt cggatgcacc gcgaaggcgg cttatccgac ctacgttctt
     4681 atatgcccag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
     4741 accacgaccg caccgatgct catggatgcc acataccagc gcagatagcc gttctcactt
     4801 aacagcagac ctttacctgc aaagcgggaa aggacagccg ggatgttcat cattgagttc
     4861 agcggatcgc gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa cactttgtca
     4921 tacagccagt caaatcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca
     4981 ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga
     5041 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
     5101 agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
     5161 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
     5221 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
     5281 gtcataaacg caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca
     5341 cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggtag tgccgacagt
     5401 gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
     5461 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
     5521 agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc
     5581 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
     5641 ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca
     5701 atacccacca gatgcagaac ttccggcgtc accaggaaca ggccgtgggt acgggcgatc
     5761 aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac aggcgtcggg
     5821 cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
     5881 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct
     5941 ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaac
     6001 agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgccgct
     6061 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
     6121 ccttcccagc cgaggtacat cagcagcagg ttatcggcga gaaccagaac taccatgctg
     6181 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
     6241 caggaggcgt acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc
     6301 gacaggccgt ccagcaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc
     6361 cacagcggct ggctgtatga ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
     6421 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
     6481 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
     6541 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
     6601 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
     6661 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
     6721 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
     6781 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
     6841 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
     6901 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
     6961 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
     7021 tgctttagca ctgattggcg taccgtcgat accctgatca ttaacaccaa ggatggcgta
     7081 aacaatcacc accagcatga tggccgacaa aattgccgga ccaatccaca cctgcggttt
     7141 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa
     7201 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
     7261 agcgcccagt gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag
     7321 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
     7381 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
     7441 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
     7501 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttt
     7561 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
     7621 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg
     7681 cggaaaaatt ccgggtacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag
     7741 ccgaccgggc aggctaccgc gcagaggtta caggctacgc aacgctcttc gccgtccggg
     7801 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
     7861 tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc
     7921 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
     7981 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
     8041 ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca
     8101 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
     8161 acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc
     8221 cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac
     8281 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cataccgcca
     8341 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gataacgttc cacacatgcg
     8401 cctggctgtt gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca
     8461 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca
     8521 gcaacgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac
     8581 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac
     8641 tgactggcac aatcgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg
     8701 taaagatgac gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca
     8761 tgtccgcaac cagctggagc gaaccgcccc agccaacacg gttaggtccg taacggttct
     8821 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccgcag gtgacaacca
     8881 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
     8941 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
     9001 aatgccggac atacccatcg gcaaacccac ctgccctgcc gtcagccctt cagcgatttc
     9061 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgagcgc ctgcgttcac
     9121 gcccaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg
     9181 gaagaccgga gcacgctgtg acaattcatc gctgccaaac aggtggtaat acggcgcgat
     9241 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
     9301 attttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
     9361 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg
     9421 cacttgcgaa cggtgcgcag tcggctggtt gttaccttcc atcgagaagg tgaacatggt
     9481 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
     9541 accgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
     9601 cggcgcagca tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac
     9661 atggtcgagc tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa
     9721 ccagcgccag ctttccagca tgacagtttt gctgtcgtaa taagcaggat cgtaaacctg
     9781 gaagaaacgt tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct
     9841 ggcagcagaa agtaccagat gggcgttttc cataatcgct gtgcgttgat gatcaaccac
     9901 catcaccagc ggcgctttag ccaacgcagc attcacgcgg gtagcggaag cgtgacgatg
     9961 cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc
    10021 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
    10081 catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc
    10141 ctgaatcacc tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
    10201 tgccagtgcc tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac
    10261 cgctggcgca gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc
    10321 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgggtgtcat caacgttggt
    10381 aacaaacagc ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc
    10441 agccactttc tgtgctgccg ccatttcgcg cgctttacct ttcacagcct gacgcactgc
    10501 cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
    10561 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
    10621 cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
    10681 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
    10741 tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
    10801 aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca ggttgacgta
    10861 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
    10921 gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga
    10981 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
    11041 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
    11101 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaat
    11161 gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt agcaggcgat
    11221 gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt
    11281 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca
    11341 gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac
    11401 cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
    11461 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
    11521 cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc agcaaaagta
    11581 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
    11641 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcagtcag
    11701 agactgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
    11761 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
    11821 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
    11881 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatcgccc ggctgacctt
    11941 caccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
    12001 tacaccagcc gcaggattca cgggcgaaaa actcttccag gttacgcacc agcgacacca
    12061 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctt cccgctttac
    12121 cgatactttc gaattccatc ggcaggtcaa gatgcgcttc ggtcaggaag tcggtgcccg
    12181 cgccgcctgg ctgccaggct ttgaatttca gaccgtcacg cataccaccg gcgtaatctt
    12241 cgaggatctc gcgcgcggta gtaccaaacg gcagttccca caggcccgga tttttcaccc
    12301 gaccggagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
    12361 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac
    12421 aggtcggttt accccatgcg ccggaggtcg ccgggaatgg aggcttcgag cgtgggttag
    12481 cacgacgtcc ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg
    12541 ccccggtatg gacgaacagt tcgaagtcaa aacctgttcc cataatattt ttgccaagca
    12601 gacctgcttc ggtggcttcg gcaatggcac ggcgtagatt cactgccgct tcgatatatt
    12661 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac
    12721 cttccaccag caggtgcggc agttgctcca tcaacaggcg gtctttatag gtgcccggct
    12781 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
    12841 ggctccactt caggccagtc gaaaagcccg cgccgccgcg ccctttcaga ccagcgtctt
    12901 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gccccttcgt
    12961 aaccattttt gctgcggtat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
    13021 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
    13081 ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca tcatatttgg
    13141 ccctttatca cagttcccca ggcagcaggt tggcagcagc gtaaagcgac cgtcaaacgt
    13201 cgtttgccct ggtttgatgt tcagtttttt ctccagcgcc gcctgaatac cctggtaacc
    13261 gttgatgtga cagaccacgc tgtcgcaata acggatcaca tggcgaccaa ccggttggcg
    13321 gaagatctgg ctatagaacg ttgccacgcc ttcgacatcg ctcgccggaa tacccagcac
    13381 atcggcgatc gcgtggatcg cgccgtccgg cacccagcca cgctgcttct gaacgatttt
    13441 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
    13501 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
    13561 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
    13621 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
    13681 cggatacgag tgcgataact catggtgcta ccgtcactgg tcaggtagta actgttgatc
    13741 cccttggtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
    13801 acttgcagga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
    13861 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
    13921 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
    13981 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
    14041 cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca ggcccgcgcc agtggtgccc
    14101 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
    14161 gtattttgca gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
    14221 tcacgcagca ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac
    14281 cacgccgggt gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga
    14341 tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg
    14401 tacagcaggt ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg
    14461 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    14521 acgcagccgc cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    14581 cgctcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    14641 atctcttcgc cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc
    14701 ggaccgaggt tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat cccccactct
    14761 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    14821 ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    14881 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc
    14941 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    15001 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    15061 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    15121 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    15181 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    15241 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat taccggatca
    15301 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    15361 accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg
    15421 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    15481 aggttggtta cggcaatacg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    15541 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    15601 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    15661 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    15721 ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    15781 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    15841 tcagcctgac gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    15901 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    15961 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    16021 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    16081 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    16141 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    16201 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    16261 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    16321 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    16381 ctggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    16441 tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa
    16501 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    16561 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    16621 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg
    16681 tagaaattct tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt
    16741 caactttaac aaaagtttct tcacattagt ttacataata tcaacaccat tagcatttaa
    16801 tgccctttca ccccagatcc ttgacgactc caggataatt agatgttgtt gaatcgtgtc
    16861 cgttgtgaag caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc
    16921 cccccaaaag agagtatttt cttgatctgt gacacgcttt tgtcattcca taacaaaaac
    16981 gcagcaacaa atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat
    17041 tatttgtgcg ttcgggacgt gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc
    17101 tgcactatgc gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca
    17161 gcggctcgtt ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcgata
    17221 tccctttcaa tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac
    17281 ggattatggt agctctccat cgcctgatag atcacctggg ccagctcatt attactggac
    17341 gggtcatagc acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc
    17401 agcacacgca ggtccgggct catcatctca accggccttg ccgtcacgcc aagaccggct
    17461 ttcactgccg cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata
    17521 tctgctttat taagcgtcgc cagcaccata tcgcgaaacg gacttggatc atccagcaat
    17581 acaagaggga tcggctcgcc tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt
    17641 ggcgaggttc gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa
    17701 tccacttcct gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc
    17761 agcgctaatt tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg
    17821 gcagattcat ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat
    17881 gagcaggcct catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca
    17941 tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt
    18001 tccagacgct gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg
    18061 gcagcaaaag tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg
    18121 ttaattatcg gacgatttgc acttatcata tattatcact tactggcggc tcatactgag
    18181 ctggttaatg ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag
    18241 tttcatccgg aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc
    18301 acattgttcc gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat
    18361 ctattaagtt actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg
    18421 tgagtccttg ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc
    18481 aatggtgagg cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa
    18541 accttttatt tataagggtc attgcgaatt atctgatgca aagttatgtt atgttaggca
    18601 aagtaatctt ctattattaa taagcacatc aaaacctttt tgaatattaa ataataatta
    18661 attagcatca tcctcattca ttaattccgc ttaacaatag tttcacacct tgcccccatt
    18721 acgacacctc ggaaatcatc acgctgttaa actctccgta atgcggaaat atcatcatcg
    18781 cgtttcatta gtgaattctt aaatgaggca ttttcacaca attatcttac agataaaaaa
    18841 accagactta caattaagaa tcagaacaat tcactatata acattgcatg tgaagcatat
    18901 acacctcatt attttgtcat tattaagtta ttaacagcac aatcgagcct tcccctctgg
    18961 caaaatctta ttctgcagac cttcaaaaca ccgtcctggg ggagtacatt gttctaagct
    19021 gacttccacg gcagggagtg gcgataacag caaaaaaggt caagattcat gtcccccatt
    19081 gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc gtggtccggt gctgaaagaa
    19141 gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac tgaacatcgg caacccagcc
    19201 ccgttcggtt ttgacgcgcc agatgaaatc ctcgttgacg tgatacgcaa cctgcctacc
    19261 gctcaagggt attgcgattc caaaggtctt tactccgcgc gtaaagccat catgcagcac
    19321 taccaggctc gtggcatgcg tgatgttacc gtggaagata tttacatcgg caatggtgta
    19381 tcggagctta tcgttcaggc aatgcaggca ttgctgaaca gcggggacga aatgttggtt
    19441 cctgcaccag attacccact atggaccgcg gcggtttcgc tttccagcgg taaagcggtg
    19501 cattatcttt gcgatgaatc ctctgactgg ttcccggacc tcgatgatat tcgcgctaaa
    19561 attacgcctc gtacgcgtgg gatcgttatt atcaacccaa ataacccaac cggcgcggta
    19621 tattccaaag agcttttaat ggagattgtg gagattgcac gtcagcataa tctcattatc
    19681 ttcgccgatg aaatttatga caaaattctt tacgacgacg ctgagcatca ctcaattgcg
    19741 ccgctggcac ctgacctgct gaccattacc tttaacggac tgtcgaaaac gtaccgcgtt
    19801 gcaggcttcc gtcaggggtg gatggtgttg aacgggccga aaaaacacgc caaaggctac
    19861 atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg ctaacgttcc tgcgcaacac
    19921 gccattcaga ccgcgctggg tggttatcag agcatcagtg aatttattac ccctggcggt
    19981 cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca acgatattcc gggcgtttcc
    20041 tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa tcgacgccaa acgctttaac
    20101 attcacgacg atcagaaaat ggtgctggat ttcctgttgc aggaaaaagt tctgttggtg
    20161 caagggacgg cattcaactg gccgtggccg gatcacttcc gcattgtcac gctaccgcgt
    20221 gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt tcctttctgg ttatcatcag
    20281 ctgtaatctt aatttcactg ccggagattg catccggcag cgttatcccg ccacaatgac
    20341 ctgatgatgt catcatacgt aaggtcacta tgaaacagag ccattttttt gcccatctct
    20401 cccgcctgaa actcattaac cgctggccgc tcatgcgcaa cgtgcggacg gaaaatgtgt
    20461 ccgaacacag tttgcaggta gcgatggtcg cccatgcgct ggcagctatc aaaaatcgga
    20521 aatttggcgg taatgtcaac gccgaacgta tcgctttact ggcgatgtac cacgatgcct
    20581 cagaagtgct caccggcgat ctccctactc cggtgaaata cttcaattcg caaatcgctc
    20641 aggagtacaa ggctattgaa aaaatcgctc agcaaaaact ggtcgatatg gttccggaag
    20701 agctgcggga tatctttgcg ccgttaattg acgagcatgc atatagcgat gaagaaaaat
    20761 cgctggtgaa acaggcagat gcactgtgtg catatctgaa atgtctggaa gaactcgcgg
    20821 ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga agcgacgctt gaagcgcgtc
    20881 gcagccagga gatggactac ttcatggaag tatttgttcc cagcttccat ctttcgctcg
    20941 atgagattag ccaggattca ccgctgtaag cagccggagt ctgcgtcgca tcaggcaata
    21001 agcgccggat gcgacatcag gctcttgtca aaacggaaac agcatcggga tcatcaccac
    21061 acaaaccgcc atcacgataa tggtgaacgg tacccccaac ttcacaaagt cactgaagct
    21121 gtaatttccc ggacctaaaa ccagtgtgtt aacaggtgaa gaaaccggtg tcataaaggc
    21181 ggcggatgct gccatcgcca cgaccatcgc gaatggataa ggcgacaccc ccatcgtttt
    21241 ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc gcggtattag agataaatag
    21301 cccaataacc gccgacaaga caaacaaaca ccccagcatc atatgtggcc cgtaaccacc
    21361 gccaatgtcc atcagccctt tcaccgccag cgcgacacct cccgttttct gtaatgccac
    21421 agcaaacggc atcatcccaa cgatcaaaat aatgctcggc cagtgaatgg atttataggc
    21481 gctttcagca tctatacagc ggaatttccc catcagcagg caggcgatga tagcggcaac
    21541 aggattagga atttcatctg tcagcattaa cgccaccatt agcaccagac agaaaatggc
    21601 atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc tcttccggta agttcagcgc
    21661 tacgaagtcg cggccctgtt tggccagcat accgatcagt ttccagttac ccacaaccag
    21721 gatgatatcg cccagcagca gaggctcatc cgccagcgaa ccttccagcg ccacgccatt
    21781 gcgctttagc cccaccacat tcagtccgta gcgggtacga aaaccaattt cgcgcaccga
    21841 tttaccaatc agttctgact caggaattaa tgaaatctct gccatgccca catcaagggc
    21901 ctggtcagaa aaatactcgc cgcgcagtac catcggctcc agcaattgct cactacaaaa
    21961 ttgccggaga tcgacatcag ccgcagacat atcaataagc aaaacgtcac gcgcgcgaaa
    22021 ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa cgccgccagc gttcaacacc
    22081 gatgacgtta gcgccataac gctcacgtaa tttgagatca tccagccgtt gacctatcat
    22141 tggggatccg gggcgaatag ccagacgtcg cgcacgcccg gtcagtcgat attcacggat
    22201 aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc ggggtctggg tatccccttt
    22261 cagcatgaaa cgcatcacta acatatacaa aatacccagc accagcacga ccaggccaat
    22321 aggtgttacg ctaaagaaac tgaagccgtg atagccttca cgcagcaatt cactgttgac
    22381 taccaggttc ggcggcgtcg ccaccagcgt catcatgccg ctaatcagcc cggcaaaact
    22441 taacggcatc atcagacgtg acggcgaagt ttgcatacgc atggcaacgc ttaacaccac
    22501 gggaataaag atagcgacaa cgccggttga actcataaac gcgccaagcc ccgcgacggt
    22561 cagcatcaac aaaaccaaca ttttgatttc actgttgccc gccactttga ccagccatgt
    22621 tcccattacg gtggcaacac cggtacggac caaaccatcg ccaataataa acaaggcggc
    22681 aatcaggaca acgttaggat cagaaaagcc ggaaaatact tcggggaccg tcagcgttcc
    22741 gcttaatgca aacgcgacaa taacaaacaa agcgaccgca tccatacgca ctctgcccgt
    22801 cgcaaacaag acgatggcaa cggccagtaa tgaaagaacc caaatcaatt caccgttcac
    22861 aacttatcct tgttaattga gggggatgac ttgattctgc cataaaaaag ccccgacgag
    22921 acggggctaa atcatgatca ggtgtttcac tgaataataa catcgccatt tggctgtttg
    22981 gtcacagtaa tttgctccag actgtggagg accaaatcga cctcattcag gcgcggggta
    23041 tctgccggag cgttaaccgc aatgacatgg caacccgccg ccaggccaga aagcacgcca
    23101 gcgggagcat cttccaccac cacacactcc tgcggcgcaa gccccagcag ctgcgcacct
    23161 aacagatacg catcaggttc tggttttccg cgcttcactc gctcagcggt tacaaacacc
    23221 tctggtgcgg gaagcccagc tattttatgg cgcgctcgcg ctaccggcat ggagccagaa
    23281 gtcacaatgg cccacggaat acctgcttta ttcaaatgat tgagtaaggc gattgccccc
    23341 ggaagcgcgg taataccttc ggtttccgtg gcctcgatgt gctccagacg cgtaaactcg
    23401 gcggcaatat cagcctcgga tttgcccgcc ataaaatggc gcaaagaggt gatcgcctgt
    23461 ttaccgtgaa tgaaagccag cacctcttcc ggcgctaacc catgacgtct ggcccagttg
    23521 ctccacgccc gttctaccgc aggcagggaa tccaccagcg ttccatcaag atcaaacaga
    23581 aaacctttgc accgcacgcg ggcctcctca ggcgttgata atttgattaa tttcgttggc
    23641 gctcaaatgg tactgacgcg ggcaggcatg ccacacatta agcatgcgct gatatttttc
    23701 ccacattggc gtctgggcgt taaaaccgtg agttccggcg tcaaaatggg tatagcgccc
    23761 ttccacatta accataaagc ggacataacc gaggtaacgt gcttcagtgg cggcgtcaaa
    23821 gccgaggaag gtgacacgac gttcatcgat ggattgctgg tcctgcaaat tagaccagga
    23881 aacatgcaac gcatgataca tctccataat gtcgatgata gtgcggcagg tttcttcttt
    23941 cagctcgcca aactcgcgat ccaattcacg catctgtaat ccgtaaccac gctcaataat
    24001 tgtttgcagg cgacggtaac gttcagcatt tgccggatcg agcatagtca tcatcttgta
    24061 ctgattagac aaaataagac gttgcgcgtt ggtcatttcc attgttgact cctgtatcac
    24121 tatactacgg tgaaaaaaaa gaaggctgag tatgccttct tttatatgcg taatcagggg
    24181 tcaattacaa atcatcaagg aaagttttat ccagttgttt gaaggcgcgc ttaagcgtgt
    24241 cagctaatgc ctggtaatca ggcttgcctt caacgggtgc caacacctgt ccagactcct
    24301 gcaatttacc gcgaacttca taaaaccagt taaggattgc agggggtaat ggcgttacag
    24361 aacgcttgcc cagccaccac aatccctgca tgggtaaact taaggcgaac agcgcagtgg
    24421 caactgccgg cccaagctga ccgcccaggg caatctgcca gcagagagta aatacggcga
    24481 tcggcggcat aaaacggatc gcataacgcg tcatcttgat aacgcgattt tcgacaaaga
    24541 ccggggcaag gcgtttttcc agcggccacg tctttgagta atgctgtccc cggcgaaaca
    24601 agctaaaaaa attaacagaa cgattatccg gcgttgacat gcttcacctc aacttcacat
    24661 ataaagattc aaaaatttgt gcaaattcac aactcagcgg gacaacgttc aaaacatttt
    24721 gtcttccata cccactatca ggtatccttt agcagcctga aggcctaagt agtacatatt
    24781 cattgagtcg tcaaattcat atacattatg ccattggctg aaaattacgc aaaatggcat
    24841 agactcaaga tatttcttcc atcatgcaaa aaaaatttgc agtgcatgat gttaatcata
    24901 aatgtcggtg tcatcatgcg ctacgctcta tggctccctg acgttttttt agccacgtat
    24961 caattatagg tacttccatg tcgagtaagt tagtactggt tctgaactgc ggtagttctt
    25021 cactgaaatt tgccatcatc gatgcagtaa atggtgaaga gtacctttct ggtttagccg
    25081 aatgtttcca cctgcccgaa gcacgtatca aatggaaaat ggacggcaat aaacaggaag
    25141 cggctttagg tgcaggcgcc gctcacagcg aagcgctcaa ctttatcgtt aatactattc
    25201 tggcacaaaa accagaactg tctgcgcagc tgactgctat cggtcaccgt atcgtacacg
    25261 gcggcgaaaa gtataccagc tccgtagtga tcgatgagtc tgttattcag ggtatcaaag
    25321 atgcagcttc ttttgcaccg ctgcacaacc cggctcacct gatcggtatc gaagaagctc
    25381 tgaaatcttt cccacagctg aaagacaaaa acgttgctgt atttgacacc gcgttccacc
    25441 agactatgcc ggaagagtct tacctctacg ccctgccgta caacctgtac aaagagcacg
    25501 gcatccgtcg ttacggcgcg cacggcacca gccacttcta tgtaacccag gaagcggcaa
    25561 aaatgctgaa caaaccggta gaagaactga acatcatcac ctgccacctg ggcaacggtg
    25621 gttccgtttc tgctatccgc aacggtaaat gcgttgacac ctctatgggc ctgaccccgc
    25681 tggaaggtct ggtcatgggt acccgttctg gtgatatcga tccggcgatc atcttccacc
    25741 tgcacgacac cctgggcatg agcgttgacg caatcaacaa actgctgacc aaagagtctg
    25801 gcctgctggg tctgaccgaa gtgaccagcg actgccgcta tgttgaagac aactacgcga
    25861 cgaaagaaga cgcgaagcgc gcaatggacg tttactgcca ccgcctggcg aaatacatcg
    25921 gtgcctacac tgcgctgatg gatggtcgtc tggacgctgt tgtattcact ggtggtatcg
    25981 gtgaaaatgc cgcgatggtt cgtgaactgt ctctgggcaa actgggcgtg ctgggctttg
    26041 aagttgatca tgaacgcaac ctggctgcac gtttcggcaa atctggtttc atcaacaaag
    26101 aaggtacccg tcctgcggtg gttatcccaa ccaacgaaga actggttatc gcgcaagacg
    26161 cgagccgcct gactgcctga tttcacaccg ccagctcagc tggcggtgct gttttgtaac
    26221 ccgccaaatc ggcggtaacg aaagaggata aaccgtgtcc cgtattatta tgctgatccc
    26281 taccggaacc agcgtcggtc tgaccagcgt cagccttggc gtgatccgtg caatggaacg
    26341 caaaggcgtt cgtctgagcg ttttcaaacc tatcgctcag ccgagtaccg gtggcgatgc
    26401 gcccgatcag actacgacta tcgtgcgtgc gaactcttcc accacgacgg ccgctgaacc
    26461 gctgaaaatg agctacgttg aaggtctgct ttccagcaat cagaaagatg tgctgatgga
    26521 agagatcgtc gcaaactacc acgctaacac caaagacgct gaagtcgttc tggttgaagg
    26581 tctggtcccg acacgtaagc accagtttgc ccagtctctg aactacgaaa tcgctaaaac
    26641 gctgaatgcg gaaatcgtct tcgttatgtc tcagggcact gacaccccgg aacagctgaa
    26701 agagcgtatc gaactgaccc gcaacagctt cggcggtgcc aaaaacacca acatcaccgg
    26761 cgttatcgtt aacaaactga acgcaccggt tgatgaacag ggtcgtactc gcccggatct
    26821 gtccgagatt ttcgacgact cttccaaagc taaagtaaac aatgttgatc cggcgaagct
    26881 gcaagaatcc agcccgctgc cggttctcgg cgctgtgccg tggagctttg acctgatcgc
    26941 gactcgtgcg atcgatatgg ctcgccacct gaatgcgacc atcatcaacg aaggcgacat
    27001 caatactcgc cgcgttaaat ccgtcacttt ctgcgcacgc agcattccgc acatgctgga
    27061 gcacttccgt gccggttctc tgctggtgac ttccgcagac cgtcctgacg tgctggtggc
    27121 cgcttgcctg gcagccatga acggcgtaga aatcggtgcc ctgctgctga ctggcggcta
    27181 cgaaatggac gcgcgcattt ctaaactgtg cgaacgtgct ttcgctaccg gcctgccggt
    27241 atttatggtg aacaccaaca cctggcagac ctctctgagc ctgcagagct tcaacctgga
    27301 agttccggtt gacgatcacg agcgtatcga gaaagttcag gaatacgttg ctaactacat
    27361 caacgctgac tggatcgaat ctctgactgc cacttctgag cgcagccgtc gtctgtctcc
    27421 gcctgcgttc cgttatcagc tgactgaact tgcgcgcaaa gcgggcaaac gtatcgtact
    27481 gccggaaggt gacgaaccgc gtaccgttaa agcagccgct atctgtgctg aacgtggtat
    27541 cgcaacttgc gtactgctgg gtaatccggc agagatcaac cgtgttgcag cgtctcaggg
    27601 tgtagaactg ggtgcaggga ttgaaatcgt tgatccagaa gtggttcgcg aaagctatgt
    27661 tggtcgtctg gtcgaactgc gtaagaacaa aggcatgacc gaaaccgttg cccgcgaaca
    27721 gctggaagac aacgtggtgc tcggtacgct gatgctggaa caggatgaag ttgatggtct
    27781 ggtttccggt gctgttcaca ctaccgcaaa caccatccgt ccgccgctgc agctgatcaa
    27841 aactgcaccg ggctgctccc tggtatcttc cgtgttcttc atgctgctgc cggaacaggt
    27901 ttacgtttac ggtgactgtg cgatcaaccc ggatccgacc gctgaacagc tggcagaaat
    27961 cgcgattcag tccgctgatt ccgctgcggc cttcggtatc gaaccgcgcg ttgctatgct
    28021 ctcctactcc accggtactt ctggtgcagg tagcgacgta gaaaaagttc gcgaagcaac
    28081 tcgtctggcg caggaaaaac gtcctgacct gatgatcgac ggtccgctgc agtacgacgc
    28141 tgcggtaatg gctgacgttg cgaaatccaa agcgccgaac tctccggttg caggtcgcgc
    28201 taccgtgttc atcttcccgg atctgaacac cggtaacacc acctacaaag cggtacagcg
    28261 ttctgccgac ctgatctcca tcgggccgat gctgcagggt atgcgcaagc cggttaacga
    28321 cctgtcccgt ggcgcactgg ttgacgatat cgtctacacc atcgcgctga ctgcgattca
    28381 gtctgcacag cagcagtaat ctcgtcatca tccgcagctt tgcgctgcgg atatctgaac
    28441 cggaaataat cactatttcc ggttttttat tctcttaatc tgcattaatc ctttctgatt
    28501 atcttgctta actgcgctgc atcaatgaat tgcgccattt cactttgcat acttaccact
    28561 ttgttttgtg caagggaata tttgcgctat gtccgcaatc actgaatcca aaccaacaag
    28621 aagatgggca atgcccgata cgttggtgat tatctttttt gttgctattt taaccagcct
    28681 tgccacctgg gtagttccgg tggggatgtt tgacagtcag gaagtgcagt atcaggttga
    28741 tggtcaaaca aaaacacgca aagtcgtaga tccacactca tttcgcattc tgactaacga
    28801 agcaggcgaa cctgagtatc accgcgtaca gctgttcacg acgggcgatg aacgcccggg
    28861 cctgatgaac ttcccgtttg aaggattaac ctcaggatcg aaatacggga cagctgttgg
    28921 catcatcatg tttatgctgg tgattggcgg cgcgtttggc attgtgatgc gtacaggaac
    28981 cattgataac ggtatcctgg cgcttattcg ccatacccgc gggaatgaaa ttctctttat
    29041 tcctgcgctg tttattctgt tttcacttgg cggcgcggta tttggtatgg gagaagaggc
    29101 cgtcgccttt gccattatca tcgcaccgct aatggtccgg ctgggctatg acagtattac
    29161 caccgtcctg gtgacctata ttgccacgca aatcggtttt gccagttcgt ggatgaaccc
    29221 gttttgtgtg gtcgttgctc aggggattgc cggcgttccg gtgctttctg gctccgggtt
    29281 gcgcatcgtg gtgtgggtta tcgccactct gattggcctg atctttacca tggtgtacgc
    29341 ctcacgagtg aaaaagaatc ctcttctgtc acgcgtgcat gagtccgacc gcttctttcg
    29401 tgaaaagcag gctgatgttg aacaacgtcc gtttaccttt ggtgactggc tggtattgat
    29461 tgtcctgacc gccgtaatgg tctgggtgat ttggggcgtg atcgttaatg cctggtttat
    29521 tccagaaatt gccagccagt tcttcaccat gggtctggtg attggcatca tcggcgttgt
    29581 tttccgcctt aacggcatga cggttaatac catggcttca tcctttaccg aaggggcgcg
    29641 aatgatgatc gcccctgccc tgctggtggg tttcgccaaa gggattttgc tgctggtcgg
    29701 taatggtgaa gcaggtgatg ccagcgtgtt aaataccatc ctcaacagca ttgccaatgc
    29761 cattagcggt ctggacaacg ctgtcgcggc ctggtttatg ttgctcttcc aggcggtatt
    29821 taatttcttt gtgacatccg gttctggtca ggcggcgtta accatgccgt tactggcacc
    29881 gcttggcgat ctggtcggtg ttaaccgtca ggttaccgtg ctggcattcc agtttggtga
    29941 tggcttcagc cacatcattt acccaacctc agcttcgtta atggcgacgc tcggtgtttg
    30001 ccgggtggac ttccgtaact ggctgaaggt gggcgcgaca ctgcttggac tgctgtttat
    30061 tatgtccagc gtcgtggtga tcggcgctca gttgatgggc taccactaaa aatgttaaga
    30121 gccgcaattg cggctctttt ttcattctgc cgtttcagtc tctacggctt catttttggc
    30181 attgcgcttc atccacaacg ccagcgcttt cagcgagtct ggagtgaact catcgcagcg
    30241 tgcggtgatt tcttccggcg tcagccagca aacttcactg acttcatctt cctgtagggc
    30301 gaagggaccg tgagagacgc agctgaacaa tgcgccccag acacggcaat ttttatcttc
    30361 gaaatagaac tgcccgtgct cagcaaaggg gacaccggca atgcccaact cttcttccgc
    30421 ttcgcgacgc gcggattcca gcagttgctc atcggcctgg accacaccgc ctgcggtcgc
    30481 atctaacatg ccgggtaaaa agtcttttgt ctcggtacga cgctggacca gaattttgcc
    30541 catgccatca tgcacgacga tgtaagttgc acgatgacgc agacactgtg cccgcatttg
    30601 ttcccggctg gcttgtgcaa tgacttcgtt ctcttcattg acaatatcca cccattcagt
    30661 acttgccaaa cgacgctgtt ccattatcag gaaaccttct ttttctggcg ctcttacggc
    30721 gcattttgag ttgtgggtaa attacggatt aatcgcgacc tgcgcaatga tactttgatc
    30781 attgagtgcg attacactaa gtacgtcatt atccagcatg ccataactcg ccggattacc
    30841 gcctttcgga atactcaccg agccggggtt gaagtggaaa atctcccccc gctgttctgc
    30901 cactggcaga tgggtatggc cgtacaccag cacatcgttc tggtttaaag caggcagatt
    30961 ttccgggcca aaaagatgac catgcgtcaa aaacagacgt tgtttttcca gtaatacctg
    31021 ttgccacggc gcggttatcg ggaaatgcag cagcatttga tccacttcgc tgtcgcagtt
    31081 gccgcgcaca gcgataacct tatgtgccac ttcattaagc cgttcagcga ctttggctgg
    31141 cgcgtaaccc tccggtaaag cattacgcgg gccgtgattc aacacgtcgc caaggatcac
    31201 cagccactgg gcaccgcttt gggcaaacaa ctccagaaca cgttccgtcg ccggtaacga
    31261 cccatgaatg tccgatgcaa acatcagttt catcactcac tcctcgtcga aaaaacgtaa
    31321 gctatgatac tggattctgt cgttgctatc agccagattg cttcgccgaa agtgcaataa
    31381 aacgctggac agacgctcgc tgccactgga atgtcgcata atccaccagg cgttctggca
    31441 cctcatcgcc atgtagtacc aggcggttaa tcattagcgc cagatcagta tcagcaatgc
    31501 accattcacc aaataaattc ggctgaccca gtactaacaa atgttctgcc atcgcaaaca
    31561 gtttctctgc actggctttt ccctcggccg ttagtggcgc ttttttcgcc cccgcaaaga
    31621 caacatccgt cggacgctct tcgcggatgg gcatcagatc gctgcgcagc caggcctgaa
    31681 tctgtcgtgc acgcgcacga ttttctaaat caagcggata aatacgttcc caggtcggtg
    31741 gcgcaaatcg atcttccaga tactccgcaa tggcagaaga ttcactcaac tcaaaatcat
    31801 cgatttgtaa taacggcaca cggcgtgtct gaccgtaacc ttgccacgtc ggctgcaaat
    31861 gttcaccgct gtcgaggtcg atggtcttga tatgaaacga caggcctttt tcctgcaacg
    31921 ccacccaggc ggataacaca taaggggaga aaaagtgggc atctgaccaa agcgtgatag
    31981 cgggtttact cattatgtcc tcggcaataa agggggctgg ctatcaaact atagagcctt
    32041 tttgtcactg tcacgtgacg aatactcacg taagtcgccg cgctttctat acttgtggtg
    32101 ttcagatgat taaacaaccg gagctgcaat gatcgatctc tatttcgccc cgacacccaa
    32161 tggtcacaaa attacgctgt ttctcgaaga agcagagctt gattatcgct tgattaaggt
    32221 agacctgggg aaaggcggtc agtttcgtcc ggaatttttg cgcatttcgc ccaacaacaa
    32281 aattccggca attgttgatc attctcctgc cgatggcggc gaaccgttaa gcctctttga
    32341 gtctggtgcc attttgttgt atctggctga gaaaacagga ctctttttga gtcatgaaac
    32401 gcgtgagcgc gccgccacat tacagtggtt attctggcag gtaggcggac tggggccgat
    32461 gcttgggcaa aatcatcatt ttaatcacgc agccccccaa accattcctt acgctattga
    32521 acgttatcag gttgaaactc agcgtcttta ccatgtactg aacaagcggc tggaaaactc
    32581 gccctggctg ggaggcgaga actacagcat tgcggatatt gcctgctggc cgtgggttaa
    32641 tgcctggact cgccagcgaa ttgacctcgc aatgtatccg gcagtcaaga actggcatga
    32701 gcggatccgt tcgcgccctg ccaccgggca ggcactgcta aaagcacaac tcggtgatga
    32761 gcgttcggat agttaacaga aacaggttct cgtgtattat ttcatcctaa gtaaaacaac
    32821 ggagaacctg caatggcaca acctgccgct attattcgta taaagaacct tcgtttgcgt
    32881 acgtttatcg gaattaagga agaagaaatt aacaaccgtc aggatattgt tatcaatgtg
    32941 acgatccact accccgccga taaagcgcgc actagcgaag atatcaacga tgcgctgaat
    33001 tatcgcaccg taacgaaaaa cattattcag catgtagaga ataaccgttt ctctttgctg
    33061 gaaaaattaa ctcaggatgt gctcgatatc gcacgtgaac atcactgggt gacgtatgct
    33121 gaagtggaga tcgataaact gcacgcgctg cgctacgccg attcggtatc catgacctta
    33181 agctggcagc gttaaccgcc atatcgggag gctgcatgaa catagtgatc accggaggga
    33241 cgggattaat tggtcgccat ttgattccgc gtttgctgga gctgggccat caaatcacgg
    33301 tagtgacgcg taacccacag aaagccagtt ccgttctcgg ccctcgggtg acgctatggc
    33361 aagggctagc cgatcaaagc aacctcaacg gcgttgatgc ggtaatcaac ctggccggag
    33421 aaccgattgc tgataaacgc tggactcacg agcaaaaaga gcgtctctgc caaagccgct
    33481 ggaatatcac gcaaaaactg gtcgatttga ttaatgccag cgacacgccg ccgtcggtac
    33541 tcatttccgg ctcggcaacg ggctattatg gcgacttagg tgaagtggtg gttaccgaag
    33601 aggaaccgcc gcataacgaa tttactcata aactctgcgc ccgctgggaa gagattgcct
    33661 gccgggcgca aagtgacaaa acgcgagtgt gcctgctgcg aaccggcgtg gtactggcac
    33721 cggacggcgg tattctcggt aaaatgctgc caccgtttcg tcttggcctg ggcgggccga
    33781 ttggttccgg tcggcagtat ctggcctgga ttcatattga tgatatggtc aacggcattc
    33841 tctggctgct ggataacgac ctgcgcgggc catttaatat ggtttcgccc tacccggtac
    33901 gcaatgaaca atttgcccat gcgctcggtc aagcgctgca tcgcccggcc attttgcgcg
    33961 tccccgcaac cgccattcgg ctgttaatgg gcgaatcttc aatactggta ttaggtggac
    34021 aacgcgcgct gcctaaacgg ctggaagaag cgggttttgc gtttcgctgg tacgatttag
    34081 aagaggcgct ggcggatgtc gttcgctgat gtggtttaca gcaaacatcc gccagttaac
    34141 tcccggtgtt acaggattag tggctttgcg cgataagatc gtctggtgaa agtcgggtca
    34201 ccatcataac taactctctg tctaaacctc tatccagcat ctcctgagca atacgcaggg
    34261 cttcttcgtg tttgccctgc attgcgcctt cttcacgtaa tctgtcagca atggtcatca
    34321 gtttctcctt ttcttgtggt gcgcgttctg ttatctcacc aataaatgca cggaaacgct
    34381 gggcatcccc tgtttgtaat acgtaattaa acagggcttt tagctgtctg tcattagtgt
    34441 tccctgtaac tagcagcgaa acaatttggt cgactaatcc caacagatcg cgctgacgaa
    34501 tatgtttctg aattaactcc aacagcgcca ttttgcggtg ttgcataatc tcgtcatccg
    34561 gcaccacggt aatatccacc aacggaaacg ctgatgaata tattttgcgg gctatagcag
    34621 gctcggcaaa ttcatcaagc cagcagagtg aataaggata aggacttctg caaccatgat
    34681 aaaacagcat cgggatcacc aatggaagct ctttatagcc cgcatcaaga tggttttgca
    34741 ttgccgcaat ggaataacgc atcatgcgaa aagccattaa ttcttccggc ttactttggt
    34801 gctctatcac tacataaata taacccactc cctcctgcgt tttcacagac cacaagaggt
    34861 cggaataata ttgccgcagg tcgtcatcaa taaaactgtt tggttccagt ttaagcgtcg
    34921 ttaaatcaca cagtttgcgc agcggcgcgg gaagatgaat atcaataaaa tcccgcgcgg
    34981 tgtctggatg gcgtaaaaaa gatttaaata ccgcatcatg cggcgtggaa gttgtcgata
    35041 ttgtcatggc gttccgtcac ccctcaaatt taagatgacg cgacaataac cacgattaac
    35101 acccaatgca gcaggcagtt tttatctttg gacagcgaat tcagaagtat ttttagcgga
    35161 ctgcaacttt tacatttcag cggaagctga ctcacaaatt ccccatgaaa atactccggc
    35221 agcgttagcc accggagagg aaattatttc agcgatccct taaggaactg ttgcagacga
    35281 gggctttgcg ggttgccaaa taactgctcc ggcgcgccct cttcttctat ttttccctga
    35341 tggaggaaaa tgacgtgagt agaaacatgg cgagcaaagc ccatttcgtg agtcactacc
    35401 accatggttt tcccctcttc tgccagttgc tgcataatac gtaacacttc gcctaccagt
    35461 tcaggatcga gcgccgaggt aggttcatca aacagtaaaa cttccggttc catcgccagc
    35521 gcccgcgcga tagaaacacg ctgttgctga ccgccggaaa gatgcaccgg atatttcccc
    35581 tgcgcacgtt cgtctatccc gactttcgcc agatacttca ccgcccgctc gcgcgcttcc
    35641 tgcttgctca ggcccaacac ctgaatcggc gcttccatga cgttttccag caccgtcata
    35701 tggctccaga gattgaagtg ctggaatacc atcgtcaggc gtgtgcgcag taagcgcagt
    35761 tgatttttat cggcgacttt gagttgacca tctttgtcgc gcaccagatt gatcgtctgg
    35821 ccattgacca cgatcgaccc ttcactcggt ttttcgagga agttaatgca gcgcagaaag
    35881 gtacttttcc ccgatcccga cgatccgatg atgcttatta catctccggc attcgcttgc
    35941 agtgagaccc ctttcagcac ttcatgttcg ccgtagcgtt tgtgcaaatc gataacgttt
    36001 aatttattct cggacatcgt attctcagtg cgttgaagaa ggtttcacat gctgcaacca
    36061 gcgtttttcc gctctgcgaa agaggctgat caggacataa gagatgatta aatagagcac
    36121 cgcggcaatg ccgaaggcgg taaaaggttg atacgtggcg gcgttaatat cgcgggctat
    36181 tttcagcaga tccggcacag tggccgtaaa cgccagcgca gtagagtgca gcatcaggat
    36241 cacttcgttg ctgtatgccg gtaacgcaat acgcagcgca gaaggcaaaa taatgcaacg
    36301 atacatttta aaagtcgaga agccataggc tctggcggct tcaatttccc catgcggaac
    36361 cgaacggatt gccccagcaa aaatctcggt agtgtaagcg caggtgttaa gcgtcagcgc
    36421 cagcacggta cagttcaggc cactgcggaa gaaagcgtta aggaattcgg ttcccttaac
    36481 aatctcaagc gtgtacatgc cggaatagaa caccagcaac tgaacataca gcggcgtacc
    36541 gcgaaaaata taggtaaata accagattgg aaactggatg tatttattac tggagacacg
    36601 accaatcgcc agaaacagcg ccaggactcc gcctatcact accgacaaaa taagcagcca
    36661 cagagtgatc gccacaccag taaagcgata accgtcggtc cacagcagcg gtttccagta
    36721 ttcatgtaag atttcgatca caggtcagcc ctcttcacac ccacggagta gcggcgctca
    36781 aggaacagca gcacaccatt ggaaacagtg gtgaaaacca ggtaaatcac gccacagacg
    36841 atggcgaaat agaacggttc ccaggtactt ttgcctgcca gttgggtggc tttgaccaca
    36901 tcttccaggc cgagtaacga aaccagtgcg gtagatttga ggatcacctg ccagttgttg
    36961 ccaatgcctg gcagggcgta acgcatcatc gccggaaaca tgatccgccg aaacacttgc
    37021 ccacgagtaa aaccgaacgc cgtcgccgcc tctatatgcc ctttcggcac tgccataaac
    37081 gcgccacgga acgtttcggt aaaataagca ccgtagataa aaccgagagt aataatacca
    37141 gcgaccatcg gatcgatatc aatctgcccg acgcccatcg cctccgtcac cgtgtttagt
    37201 gcaatctgca aaccgtagaa aatcagcagc atcaacacca agtccggcac gcctcgaatc
    37261 agcgtggtgt acccttcgaa aatcagcccc gaaagccgat tttgcgagag cttaccgcca
    37321 gcgccaatta aaccgatgat tacagcgagc actacagaac tgatagccag ctccagcgtg
    37381 acgagcgcac cctgtaaaat aacacctgaa aacccataca acatgctgcc tgtcctgtcg
    37441 tgtgtggtga attaccgcct cttgtctccc tccggttacc cgaagggagc gggctttctt
    37501 tcaccgatgg cgattaaccg ccataaacat caaaatcgaa gtactttttc gctaatttct
    37561 cgtaagtacc gtcagcgcgc atttcggcaa aggctttgtt cagtgcctcg cgcagttcgt
    37621 tatcttcttt acgcaggccc atgccggtcc ctacgccaaa cagtttttca tctttaacag
    37681 acgggccacc gaatttgtaa tctttaccga caggttgttt gaggaaacct tcgctggcag
    37741 cgacctcatc ctggaacgcg gcatcaatac gtccggcagt caggtcagaa taaatgttgt
    37801 cctgcccctg atacgagacg atttcaatgc cttttggtgc ccaatgttca ttaccgaacg
    37861 tctcctgggt ggtgccctgc aataccccta cccgtttgcc tttcagcgac tcgactgtcg
    37921 gctgaatgtc ggaatttttc gccaccacca aacgagaatc ggcagcgtac agtttgtcgg
    37981 tgaaggctat ttcttgctga cgtttttccg taatggaaag cgatgacatg atggcgtcaa
    38041 tcttcttcgc ttttaaggac gggattaacg catccagcgg attttcgaca aacgtacatt
    38101 gcgtattgat gcgtttgcat aattcctttg ccagatcgat atcgaagcca accagttcgc
    38161 cttgtgaatt ttttgattca aatggcgcat aggtcgggtc ggtaccgatg cggatgtttt
    38221 gcggaatcgc agcaaacgcc gcagttgcgc tggagaaggc cagaaccaga gagagcgata
    38281 gcaccagttt tttcataatg tcctcaactg acagtctttt tatagggaat atttacaggt
    38341 ttgttacact tatcgtgcca taaaattgac catcaaggca aaatatcccg ccccgttaga
    38401 cgtattattt ttcattaaaa atgcttaagt atgcatttca atgcaccacg atagtgcaac
    38461 aaacctggcg tgcaccatga tgatcattcc atcaggtaca gcttcccagc gacgtatcag
    38521 tcaccgtaga cattaaagtc gaaatacttt ttcgccatct tgtcgtaggt gccgtcctga
    38581 cgcagctcgc caagcgcctt attgaaggca gccgtcagtt cagcatcatc tttacgtagc
    38641 cctacaccgg tgccatcacc gaagtatttt ttgtctttta ctgatgagcc agcaaaggcg
    38701 aaatctttac cagcaggttg cttgaggaat ccttcgctgg cagcaacttc atcttgtaac
    38761 gcagcatcca gacgtcctgc agccagatcg gaatagacca aatcctggtt ggcataggcc
    38821 accacatcca cgcctttgct acgccaggtc tcgttagcgt aagcttcctg ggttgatccc
    38881 tgcagcacac caacatgttt acctttcagt gaatccagcg ttggctgaat cggtgaacct
    38941 ttggcagcaa tcaaacgaga atctgcggcg tacagcttgt cggagaaggc aatctcctgc
    39001 tgacgtttat cggtaatgga aagcgacgaa ataatggcgt cgattttttt cgctttcagt
    39061 gaggggatca gcgcgtcaaa gtcactggca acccaggtac atttcacctg catccgtttg
    39121 cacatctcgt taccgagatc gatatcaaag ccaacaaaat cacctttagc atctttcgac
    39181 gagaacggcg cataggtggt atcggttccg atacgtaccg tctccggtag cgccgcatag
    39241 ctggaagccg ctgcggagag accgactaac aaagacagag cgagaatcga cttcttcata
    39301 cataaccctc aagtgaaaat ggctttttta tgttgtgtat tgtgttgtgt gtttgcaggc
    39361 tctttcatgc aggtcttatg ccatcttgac gacaacagta acattcaacg ttaaatatgt
    39421 taataagacg ttgcattatt gtcctgaagt tgaagatagc aggtatagcg gttgaatcgc
    39481 agcgtttcga ttgaatggca gaaacaaaat gtcgaggatt tgatcgcggt tgcaaaattg
    39541 ccctgaaaca gggcaacagc ggagttatgc gccctgccag cgggcaaaga gatcttcagg
    39601 aagggttatt gcaaactggt caagaacacg attaaccgtc tgatttatca catcatcaag
    39661 ggattgcggg cgatgataaa acgccggaac gggaggcata atcaccgcac cgatttctgc
    39721 cgcctgagtc attaaacgca gatggcctaa gtgcaatggt gtttcacgca cgcagagcac
    39781 caacgggcga cgctctttca gcaccacatc tgccgcacgg gtcagtaagc catcagtata
    39841 gctatggaca atgccggaaa gggttttgat tgaacagggt aaaatcacca tccccagcgt
    39901 ctggaaagaa ccggaagaga tgctggcgga aatatcgcgc gcatcgtgcg tgacatcagc
    39961 taatgcctgc acttcgcgca gggaaaaatc cgtttcgagg gataaggtct ggcgcgccgc
    40021 ctggctcatc accagatgcg ttttgatatc tgtgacatcg cgcagaacct gtaataagcg
    40081 cacgccataa atcgcgccgc tggcaccgct gatgcctaca atgagtcgtt tcataaatag
    40141 ttgccctgtc agacttgcag gcagactttg cagtatttcg gcgggagttg caagtcaggg
    40201 tgccagaccg gcaccctcag cgaaggcatc atccttcgtt atgcatttcg agattttcca
    40261 cttcgttctg acgttgcact gctttggcgt catcattacg taacgtatcg aggaaatcga
    40321 ggtagccctg atcaacatct ttggtgacgt agacgccgtt gaacaccgag cattcaaact
    40381 gctggatatc cggattttca gcgcgaacgg cgtcgatcag atcgttcaga tcctggaaaa
    40441 tcaacccgtc agcaccgatg atctggcgaa tttcatcaac ttcgcgaccg tgagcgatca
    40501 gttccgtggc gctcggcata tcaataccat aaacgttcgg gaagcgaatt tccggtgccg
    40561 cagaagcgag gtacactttc ttcgctccgg cttcgcgtgc catctcgata atctgctcag
    40621 aagtggtgcc acggacgatg gagtcgtcga ccagcaggac gtttttatcg cggaactcgg
    40681 cgcggttggc attcagttta cggcgcacgg acttacgacg cagctgctgg cccggcatga
    40741 taaaggtgcg gccaacatag cggtttttaa cgaagccctg gcggtacggt ttgcccagaa
    40801 tacgagcaat ttccagcgcg atatcacacg aggtttctgg gatcgggatc accacgtcga
    40861 tatccagatc ttcccattcg cgggcaattt tctcgcccag tttcgtgccc atattcacac
    40921 gcgcgctgta aacggaaatt ttgtcgatga acgagtccgg gcgagcaaag tatacatact
    40981 caaacaggca cggattgctg accggattgt cagcacattg acgggtaaac aactgccctt
    41041 cttcagtgat gtaaatcgct tcgcccggcg cgacgtcacg caggaaatca aagcccagcg
    41101 tatcgagcgc tacgctttcg gaagcgacca tatattctgt acggttctcg tcaatatcac
    41161 gttttcccag taccagcgga cgaatcccgt ttggatcgcg gaaagcaacc ataccgtggc
    41221 cgataatcat cgccacacag gcatatgcgc cgcggattaa gcggtttgtg gcagcaatgg
    41281 cagcgaaaat attgtcggct tccagcgggt agtggcggaa gttgtccagc tcgctagcga
    41341 agatattaag cagaatttcc gagtcggaag tggtgttgat gtggcggcgt ttttcttcaa
    41401 acagtttttt acgcaactcg tgagcgttgg tcagattgcc gttgtgggca agcgtaatgc
    41461 catacggaga gttaacgtaa aacggctgcg cttcagaggc gctggagctg ccagccgtag
    41521 ggtaacgcac atgaccaatg cccatattgc cctgcaaacg ctgcatatgg cgagcttcaa
    41581 atacatcgct caccagcccg ttcgctttac gcaaacggaa gcagttattg gcatctatgg
    41641 tgatgatgcc ggcggcatcc tgaccgcgat gctgaagcac cgttaaggca tcataaatcg
    41701 actggttaac cggcataaca ccggcgatac cgacaatacc gcacatacgt ctttttcctc
    41761 gttaagccac atctcagagc acttacgctc tgggcaagaa acttgacgag ctttgcagat
    41821 aatcaaaaaa ccatctgatg ataaaactga attgcgggat cagctgtgat ttgctccagt
    41881 cttcgctttt cgacaccccg gtaaaggagt cgagaaagaa gagaatggcg gcaacaatca
    41941 acacaccgcg caacgcaccg aaacagacgc ccagcacccg atcggtgcct gacaaccccg
    42001 ttttctccac caactggcct atcacgaagt tcacgatagc accaacgatc agggtagcga
    42061 taaacagtac cgcgatggca attccatttc gaaccagttc gtcttcaaag cccgtaaacc
    42121 agactgacag gtaagtgtag taatgactgg caacaaagaa agcacaaccc catgtcacca
    42181 gcgataacgc ttcacgaaca aagccgcgga tcaggctaac cagagaggaa aaagcaatca
    42241 ccgcgattat ggcgtaatca atccagacca tatgtgtccc acgattttac gccctgtcat
    42301 cctgttcggg gcgcattcta acagaaaaag aaaacgtttg cgtagggatt tccttcccgc
    42361 gcatcaataa aaatggcgct gaaaaaatat tcaacgccat cgacttttta tgcctttgcg
    42421 gcatcgtgca atgcgtgtcg gatgcggcgt aaacgcctta tccgacctac ggttctaaca
    42481 ctgcccaggc ctgataagac gcgccagcgt cgcatcaggc aagactgtat taattcggcg
    42541 tatagcccat taccacgcca cttaagccag aaagttgctt caactcaccc agcgaacctt
    42601 tcagcttatc tttcgaggca tccggcccaa ccagaatacg ggtaatttta ccctgcactg
    42661 gcgtggatgg cgacgtataa acccgataac cggcaccgcg cagcttaccg acaatctcat
    42721 tcactttatc ggcatttttc agcgcaccca gttgcacaac ataggcttta cccgttggcg
    42781 ctgctttttc ttccacgacc ggcttcggtt ctggcttcgg cgcaggtggt gcttcaacct
    42841 tcggtttagg cggctccacc ggtttcggct tcggtggggc gaccggtgca ggttccggtt
    42901 caaactcggt gttattggct gcaatagtgg tcggatcgag cgacggtgct gcggcatcac
    42961 ctgcccgcac ctcttccgct gcgccttccg gcggctgcgt cggcaacgcc tgggtggcgg
    43021 ctggcatcat atcaggctca tcgcgatcgc ccgctttcgg caccagcggg atagccgcga
    43081 actcatcctg ataatgtttt ttctgcccgt ccagcagccc tggaagtaca atcaccccca
    43141 gcgccaccag cacgatcgtg cccactaacc gattctgaaa cttacttgcc accgcttctc
    43201 ctcgcgtcaa tcacttccat gacatgtgcg acagtgtgga aagaaccaca caccagcacg
    43261 gtgtcttccg ctttagcgtc cgccattgcg gcatcccatg cctgcgcaac gctatcaaat
    43321 gatttgccgt tacccaaatg ctcaagcagt tgttctgccg tggcaccgcg cggcccttcc
    43381 agtggcgcac aataccagtc atcaaccacg cttttcaacc aggccagagt tccggcaata
    43441 tctttatcat gtagcatacc gataaccgcc agcacgcgcc cgtttttcgg tagcgctttc
    43501 atacgcccgg tgagatattc cgccgcatgt ggattatgcg cgacatcaaa aataacgcgt
    43561 ggcgactcgc tcacaatctg gaaacgtccc ggcaaaattg cgctggcaat cccgtcgcga
    43621 atggcatttt cactgacttc cagcccgctg gcacgcagtg ccgccagcgc cgttgcggca
    43681 ttcggttgcg ggacaagcgg caacggcaga ttttccagcg tgccgtgagc atcgctaaac
    43741 gcccagtcat gatcggtgac ggaatagttc cactcaacgc cccgacgttg taacagtgca
    43801 cctttttcct gtgccacatc agcaatggta gaaggcattt ccggctcacc gacaattgcc
    43861 ggtttttcgc tgcggaagat gcctgctttc tcgcgaccaa tactttcgcg atctggaccc
    43921 agccagtcgg tatgatccag cgcaatactg gttactaccg cgacatcggc atcgacaata
    43981 ttggttgcgt ccagacgacc gcccagccct acttccagaa tcaccacgtc aagttgtgcc
    44041 tgcttgaaca gccacaacgc cgacagcgta ccgtactcga aataggtcag ggaaatatca
    44101 ccgcgtgccg attcaatctc cgcaaaagag gcggtgtggg ccgattccgg caattcctgg
    44161 ccctgcacac gtacgcgctc ggtataacgc accagatgcg gtgaactgta gacgcccact
    44221 ttgtaccctg ccgccatcag aatcgactcc agcgtacggc aggtggtgcc tttgccattc
    44281 gtacccgcaa cggtaaacac aaatggcgct ggtttcagaa cgccaagacg cgccgcgacc
    44341 tggctcacgc gctcaaggcc gagatcgata gttttactgt gcaggttttc cagataagaa
    44401 agccacgaag ccagaggcga cgcggcttga ggagtgcgtt tgataatcat ggtatccgct
    44461 gattcgttac ggtgagaata gcaaaagggc agagccagtg gccctgccct tatcagttat
    44521 caggcctcag gttcctgatc cggtaccggg ggtaccacta cgccttcacg cggcgcttca
    44581 ggattcggcg ctggcagatt catcaacttc gccagaatgc tcgccagttt caggcgcatt
    44641 tccggacgac ggacgatcat gtcgatcgcg cctttctcga tcaggaattc actgcgctgg
    44701 aatcctggcg gcagtttttc gcgaacggtc tgttcgataa cacgcggacc ggcaaagccg
    44761 attaacgctt tcggttcagc gatgttgaga tcgcccagca tggcgaaact tgcagaaaca
    44821 ccgcccatcg tcgggtcggt cagcacggag atgtacggca agccgcgctc ctgcattttt
    44881 gccagtgccg cagaggtttt cgccatctgc atcagcgaca tcagtgcttc ctgcatacgt
    44941 gcgccaccag aggcggagaa gcagatcagc gggcagttat cttccagcgc ctgctcaacg
    45001 gcacgcacga aacgtgcacc cacaacagac cccattgaac cgcccataaa ggcgaactcg
    45061 aatgccgcag cgacaaccgg cattccatac agagtgcctt tcatcaccac cagcgcatct
    45121 ttttcgccag tttctttctg cgcagatgcc agacggtctt tatacttctt ggagtcacga
    45181 aacttcagca catctttcgg ctcaagctcg ctacccagct ccacaaggct tccttcatct
    45241 aacaggctat gcaggcgatt acgcgctgtc atacgcatgt gatggtcaca cttcggacag
    45301 acctcaagat tacgttccag ctcagcgcgg tataaaacct gaccgcagct atcacactta
    45361 gtccacaccc cttcaggaat gctcgccttg cgggtgggag taatgttgct tttaattcgt
    45421 tcaatccagc tcattaggga cctttctgtc tgaacctggt tcgatgccag ttttatcttt
    45481 ggggacgcat aatgccattt ttgcccccaa cagaccatga atgttgcaca ttaaaacata
    45541 acagcccgaa actttggata aaaaagtggt cgaaccgcgg agttactttt tattttgcgg
    45601 cgcgtgccgc agcgcgtttg tgacgaataa attcgaaaac cccaggcaat attgaaacaa
    45661 aaataatgcc aacaatcatc agcttaaggt tgctctgaat gaaggggagc gtgccgaaga
    45721 aataacccgc gtaggtaaaa atcagtaccc acaacagtgc gccgatcacg ttataagcgg
    45781 cgaaatgacg gtacgacatg tggcccattc ccgcaacaaa cggcgcgaac gttctgacga
    45841 tcggtacaaa acgggcgaga ataatcgttt tgccgccatg tctctcgtaa aactgatgag
    45901 tcttatcaag ataactgcga cgaaagattt tcgaatccgg gttactgaac agtttgtcac
    45961 caaataaccg tccaatggta tagttcactg catcaccgac aatagcggca atcaacataa
    46021 gcaccaccat catatggaca ttgagatcgt tagtttccag cgacgccaat gcaccagcaa
    46081 caaacagcag cgaatcacca ggcaggaatg gcgttaccac cagccccgtt tcgcagaaca
    46141 gaatcaaaaa cagaatggca taaacccaga cgccgtactc cgcgaccagc tctgccagat
    46201 gcacgtcaat gtgcaggata aaatcgatga ggaaataaat caggtccata tttgcctatg
    46261 ccttgtactc gtcatctttc aggctgtaac tacgttggct acagcctgaa atgttccgag
    46321 cattattctc gttagtccgc cagaaatagc gggcccattg gcggttttgg aagatcatac
    46381 cggtcagggt aatccaccgc gaccagatac agcccttccg cttttgccgt tgctgccgcc
    46441 agcgttctgt cctttgccgc cagcaactct gctatccagc tctccggctg gttgtgggca
    46501 ccgacttcca tcaggctgcc gacaatattc ctgaccatat gatgtacaaa ggcattcgct
    46561 ttgatatcta ccaccacata aggaccgtga cgcatgacat taatatgcat aacgttgcgc
    46621 cacggggttc gggactggca ctgcaccgca cggaacgagg tgaaatcatt ctcgcccagc
    46681 aagcattgcg cagcccgatg catccgttca gcgtccagcg gttcgtaaaa atgggttacc
    46741 cctttactca gtaccgccgg gcgcagccga tgattgtaga tgatgtagcg ataacggcga
    46801 gccgtggcgc taaatcgggc atgaaaatca tcaggtacag ctttaaccca acgcacggcg
    46861 atgtcaccag gtaaattcgc atttacgccc aaggtccacg ccgcgtcttt gcgctgcgcg
    46921 gttgtttcga aatgcaccac ttgcccggta ccgtgtaccc ctgcgtcagt acgcccggcg
    46981 cagaagacgg tgatgggttc gttcgccacc tgggagagcg ccttttccag cttctcctgc
    47041 acactgcgga cttcattctg ccgttgccag ccgtaatact tactgccgtc gtactcaatg
    47101 cccagcgcaa ttttataaac tggcagttgt tgctggtcgg acattagtac agatactcct
    47161 gcaccagttt ctcggcgatt tttactgcca tcagcgcgcc gccaaagcga acgttatcgg
    47221 ccaccgacca gaactggact tgctccggca taccgtagtc attacgcacg cagccaacag
    47281 aaagatgcgg cgtacccgaa gcatccccta cctgagtcgg gaattcgttc tcttcagaga
    47341 gcacaatatc ttcgccctga gcaaacgcat cacgcgcttc ttctgccgcc agcggacgca
    47401 gtgcttcaaa gttgaccatc tgggcatgac cgtagaatac cggtgcctgg acgacgcttg
    47461 ccgaaatcat cagcccttcg tcctgcagga ttttgcgtac ttcgtcaacg atacgacgtt
    47521 cttcacgcac gctaccttcg ctatccggca acaacggcag catattgaac gccagttgac
    47581 gaccgaagaa atcttcttcg tcaatcggaa tgccgttgag caatttcgca ctctgccccg
    47641 ctaacgcatc gaccgctttt ttgccctggg cggaggctga aatcaggctg gtaacactga
    47701 tacgcgacaa accgccctga tcgattaacg gtttcagtgc cgccagcagc tggctggtca
    47761 gactgtctgg tacggcgatg acattccggt tgcggtaatc ggtcagtaca aacgggttta
    47821 cttccggcac caccagcggt acgtcgggtt cgagagcaaa cagtccgctg ctgtcgatca
    47881 ccaggcaacc tgagttggtc gcttcttcaa cccaggcagc ggtagcttct ttgcctgcga
    47941 caaaaaatgc cagctgcgcc tgcgtccagt cgaattcagc ggcatcctgc acggtgattg
    48001 ttttaccacc aaagcgcagt tgttcgcctg cgctttcgtt acgtgccagt gcataaattt
    48061 ccccaaccgg gaactgacgt tcagccagcg tttcaagcag ggcttcgccc acagcgccag
    48121 ttgcgcccag gacggcaatg ttccagcctt cagacatggt ggtttactcc agaaatagca
    48181 aagctccctg ccaatgttac agcagagagc atgaagaaga gattaacgtg ccggatgatg
    48241 aacggcgtta aaacccagtt tacacagcaa tgatgccgca ctggcgtcat cacaaattac
    48301 atacagagat gaccattcac ggcgctcaag atagtttttg cgcagtttat cgaactcacc
    48361 cggtatcccg gcgactttac gcagcggtgc gtcatcgcgg cgcacatcat acaccaaatg
    48421 caccagcctt ttcagcgtcg gttgatcgag cgggccatgc agcgtaatgc gaccaaactc
    48481 tggcgcgggc agtaatgtat ccagcgcaac gtgctgttca tgcccaataa acttgctata
    48541 ggcttcaaac acttgcgtag taccgcgtgc tttaccttcc agggtataac ctgcgatatg
    48601 cggcgtgccg atatccactt ttgtcagcaa ctccacgtta agttccggtt cgccttccca
    48661 gacatccagc actacgctta acttctggcc ttcattcagg caggtcagca acgcagtatt
    48721 atcgacgact gcgccacggc aggcgttaat cagaatcgct cccggcttca ggctacggat
    48781 cagtttttca tccgccagat gcagcgtttt gtacggccca tctttgaaga gtggcgtatg
    48841 gaaagtcaga atatccgcat gctgaactaa ttcatccagc gagcggaaat caccctcatc
    48901 cccacggtca gcgcgaggcg gatcgcaaag caatgtttta atccctaacg cttccagtcg
    48961 cgcctgcaat cgacgtccaa cgttaccaac gcccacgatc cccaccgtac ggtcgtgcag
    49021 tgaaaatcca tcgcgttcgg caagcatcag tagtgaggag aaaacatatt ccaccaccgc
    49081 aatcgcatta cagccaggtg cagcggaaaa accaattccc gcctgcttca accatgcttc
    49141 atcgacatgg tcggtccccg cagtggcagt gccaacaaat ttaatgggtt ttcctgccag
    49201 caaagattca ttcactttcg tgaccgaacg caccatcagc gcatccgcgt ctgccagttg
    49261 agcaacgggg attggacgcc cgggaaccgc ggtcacctca cccaaacggc taaataagtc
    49321 gcgggcataa ggcatatttt catcaacaag gattttcacg tttgtgttac ctgtatgaga
    49381 cgagagttaa ccggacaagt gtgccataat ctcgcggcca ggcatacttg cgaagatttc
    49441 aggtataagg atacgtaatg atacaaccta tttccggccc tcctcctggg caaccaccag
    49501 gtcagggaga taacctgccg tctggcgcgg gcaatcagcc tttatccagt cagcaacgta
    49561 cttcgctgga aagcttaatg acgaaagtga cctcactgac gcaacaacaa agagcagaac
    49621 tgtgggcggg tatcaggcac gatattggtc tgtcgggaga ttcaccgctg ctttcgcgtc
    49681 acttccctgc cgctgagcat aatctggcgc aacgtctgct ggccgcgcaa aaaagccatt
    49741 ctgcccgcca gcttttagcg caattagggg agtatttacg tctggggaat aatcgtcagg
    49801 cggtcacgga ttatatccgt cataactttg gtcagacgcc gctgaatcag ctctcaccgg
    49861 agcaattaaa aaccattctc accctgttgc aggaagggaa gatggttatt ccgcaaccac
    49921 agcagcgcga ggcgaccgac cgtcctttat taccggcgga gcacaatgcg ctaaaacagc
    49981 tggtgaccaa acttgcggcg gcaacggggg aacccagcaa acagatctgg caatcgatgc
    50041 tggaactttc cggggtgaaa gatggcgagt taattccagc gaaactgttt aaccatctgg
    50101 tgacctggtt gcaggcgcgc cagacgttaa gccagcaaaa tacgccgacg ctggaatcac
    50161 tacagatggc gctaaaacaa cctttagatg ccagtgaact ggcggcgtta tcggcatata
    50221 tccagcaaaa atatggcctt tctgcgcaat catcgctttc ttctgcccag gccgaggata
    50281 ttcttaatca gctttatcaa cggcgggtta aagggattga tccgcgtgat atgcaaccgc
    50341 tgcttaatcc ttttccaccg atgatggaca cgttgcaaaa tatggcaacg cgtcccgcgc
    50401 tgtggatact gttagtcgcg attatcctga tgctggtctg gctggttcgt tagccccgac
    50461 gaaatgacag tatcgtgaca ataattccca gcaccgcaga gatcgccccg gcaagaaata
    50521 ccgaagagta accaaacgtg gtcgccagca ttcccgccag cggcccggag acgccgaggg
    50581 cgatatcctg aaacgcggcg taaccgccca gtgcggtgcc gcgaacttgt gaggggacgc
    50641 gtttaaccac ctccacgccc agcgcaggaa agataagcga acatccggct ccggttaacg
    50701 ccgcgcccgc taatgcgacc cacgcacctg gggcttgcca gagcagcaac aagcccaccg
    50761 tttctacaag cagagagaca atcgccactt tcacgccgcc aaaacggtcc ggcatccagc
    50821 caaacatgac gcgcatcacg acaaatgcgc cgccaaacgc ggtaagagta aagcccgcca
    50881 tcgcccatcc tttgctggca aagtagagcg aaacgaaagt cccgataacc gcaaaaccaa
    50941 cgccttgtag tgccagccct aaccctggtt tccagataaa cccgacaacg ctccacagcg
    51001 atggacgttc tcccgccagg gccggtactt tgcgcactgt gccgttacag gcccacgcca
    51061 gtaagggtaa taccattgtg gtgatcgcca gtgcggcaaa accgtaatgg ctatgaatca
    51121 acaggccaag cggagcacca acagcgaggg caccgtaaat cgccattcca ttccatgaca
    51181 tcactttgcc agagtgtttt ggccctacga tgcctaaccc ccaggtcaga gcgcctgtca
    51241 gtaactggct ttcaccaaac ccaagaatca aacgcccgat gaccaacagg gcaaatttga
    51301 acggtgcgga gacaggcaaa atcgccgcca gcagcaacgc gccgccagcc agaccacaag
    51361 ctaacatccc ctgaagcgcc gaacgttttg caccatattg atcggccagt cgcccggcat
    51421 agccacgcgt cagcaccgta gccagaaact gaatcccgac ggcaatcccg accatggtat
    51481 tgccatagcc cagatcatga tgaacaaaca gcgggataac cggcaacggc agccctacgg
    51541 tcatgtaggt gagaaaaacc gcaaaagcga tgcggaagag cgaaaaattg gcagaagatc
    51601 gtgtttcggt ttggcttaca gcagtcatgc attactccag aatgcagcgc aaggcgagga
    51661 gtatccccgt ctcatctctc tggtttcagg gttacggtgc gttggcagga tttaacgcgt
    51721 acgtcttttc agaaggaaat cgacaaagcg ggaagtttgc ctggaacagg cggcgattgt
    51781 caatgatgtg aaaaagggaa ccatcaggtt cccttttgcg ttagtgccgg aggccgcatt
    51841 agcgtgtaac gtcggatgcg acgctggcgc gtcttatccg acctactccg actactccgg
    51901 cctactgcga attaatcttt cagcttgcgc attaccagcg tggcgttggt gccgccgaag
    51961 ccgaagctgt tagacataac ggtggtcagt tcgcgatcgg tcgtttcggt cacgatgttc
    52021 agacctgcag cctgctcgtc cagctcttca atgttgatgc tcggggcgat aaagccgtgt
    52081 tccagcatca gcagagagta gatagcttcc tgtacgccag cagcgcccag agagtgaccg
    52141 gtcatggctt tggttgcaga aatcgccggg ctcttatcgc cgaacacttc acggatagct
    52201 gccagttctt tcacgtcgcc aaccggagtc gaagtaccat gagagttcag gtaatcgatt
    52261 ggggtatcaa cgccgtgcat cgccatcttc atgcagcgta ctgcgccttc gccagacgga
    52321 gcaaccatgt ctgcaccatc agaggttgcg ccgtagccaa cgatttcagc atagatgtga
    52381 gcaccacgcg ccagcgcgtg ttccagctct tcaaccacta ccataccgcc gccgccagcg
    52441 ataacgaaac cgtcacggtg agcgtcgtaa gtacgggagg ctttttccgg ggtgtcgttg
    52501 tatttagtag acagcgcacc catcgcgtcg aattcgcagg ccatttccca gcacagctct
    52561 tcgccgccgc cagcaaacac gatgtcctgt ttgcccagtt ggatctgctc tactgcgtta
    52621 ccgatacagt gtgcggaagt cgcacacgcg gagctgatgg agtagttaac gccatgaatt
    52681 ttaaacggcg tggcgaggca ggcagaaacg ccggatgcca tcgctttggt gaccacatac
    52741 gggccaaccg ctttcaggcc gcgcgggccg cgcattgcgt cagcgccgaa cacctggaaa
    52801 cgcggggaac cgccgccaga acctgcaatc aggccaacgc gcgggttatt ctggtaagct
    52861 tccggagaaa ggcccgcatc tgcgattgcc tgctccatag aaaggaatgc ataaatggat
    52921 gcgtcgctca taaagcgcac aactttgcgg tcaatgaggc cagtggtatc cagttttacg
    52981 ttgccccaga cgtggctacg catgccggaa tccttcagct cctgagagaa agtgatccct
    53041 gaacgtcctt cacgcagaga tgccaggact tcctgctggt tattaccgat gctggaaaca
    53101 atgcccaggc cagtaatcac tgcacgtttc attcaatacc tctgtaagtc gcacatagag
    53161 taagtttcga atgcacaata gcgtacactt gtacgccgaa caagtccgat cagccattta
    53221 atagagaaat ttgcgcagcc ttacacacat cgctaagatc gagccaccgc ctgtaagacg
    53281 agtaacttac gtgaaacact actccataca acctgccaac ctcgaattta atgctgaggg
    53341 tacacctgtt tcccgagatt ttgacgatgt ctatttttcc aacgataacg ggctggaaga
    53401 gacgcgttat gtttttctgg gaggcaacca attagaggta cgctttcctg agcatccaca
    53461 tcctctgttt gtggtagcag agagcggctt cggcaccgga ttaaacttcc tgacgctatg
    53521 gcaggcattt gatcagtttc gcgaagcgca tccgcaagcg caattacaac gcttacattt
    53581 cattagtttt gagaaatttc ccctcacccg tgcggattta gccttagcgc atcaacactg
    53641 gccggaactg gctccgtggg cagaacaact tcaggcgcag tggccaatgc ccttgcccgg
    53701 ttgccatcgt ttattgctcg atgaaggccg cgtgacgctg gatttatggt ttggcgatat
    53761 taacgaactg accagccaac tggacgattc gctaaatcaa aaagtagatg cctggtttct
    53821 ggacggcttt gcgccagcga aaaacccgga tatgtggacg caaaatctgt ttaacgccat
    53881 ggcaaggttg gcgcgtccgg gcggcacgct ggcgacattt acgtctgccg gttttgtccg
    53941 ccgcggtttg caggacgccg gattcacgat gcaaaaacgt aagggctttg ggcgcaaacg
    54001 ggaaatgctt tgcggggtga tggaacagac attaccgctc ccctgctccg cgccgtggtt
    54061 taaccgcacg ggcagcagca aacgggaagc ggcgattatc ggcggtggta ttgccagcgc
    54121 gttgttgtcg ctggcgctat tacggcgcgg ctggcaggta acgctttatt gcgcggatga
    54181 ggcccccgca ctgggtgctt ccggcaatcg ccagggggcg ctgtatccgt tattaagcaa
    54241 acacgatgag gcgctaaacc gctttttctc taatgcgttt acttttgctc gtcggtttta
    54301 cgaccaatta cccgttaaat ttgatcatga ctggtgcggc gtcacgcagt taggctggga
    54361 tgagaaaagc cagcataaaa tcgcacagat gttgtcaatg gatttacccg cagaactggc
    54421 tgtaggcgtt gaggcaaatg cggttgaaca aattacgggc gttgcgacaa attgcagcgg
    54481 cattacttat ccgcaaggtg gttggctgtg cccagcagaa ctgacccgta atgtgctgga
    54541 actggcgcaa cagcagggtt tgcagattta ttatcaatat cagttacaga atttatcccg
    54601 taaggatgac tgttggttgt tgaattttgc aggagatcag caagcaacac acagcgtagt
    54661 ggtactggcg aacgggcatc aaatcagccg attcagccaa acgtcgactc tcccggtgta
    54721 ttcggttgcc gggcaggtca gccatattcc gacaacgccg gaactggcag agctgaagca
    54781 ggtgctgtgc tatgacggtt atctcacgcc acaaaatccg gcgaatcaac atcattgtat
    54841 tggtgccagt tatcatcgcg gcagcgaaga tacggcgtac agtgaggacg atcagcagca
    54901 gaatcgccag cggttgattg attgtttccc gcaggcacag tgggcaaaag aggttgatgt
    54961 cagtgataaa gaggcgcgct gcggtgtgcg ttgtgccacc cgcgatcatc tgccaatggt
    55021 aggcaatgtt cccgattatg aggcaacact cgtggaatat gcgtcgttgg cggagcagaa
    55081 agatgaggcg gtaagcgcgc cggtttttga cgatctcttt atgtttgcgg ctttaggttc
    55141 tcgcggtttg tgttctgccc cgctgtgtgc cgagattctg gcggcgcaga tgagcgacga
    55201 accgattccg atggatgcca gtacgctggc ggcgttaaac ccgaatcggt tatgggtgcg
    55261 gaaattgttg aagggtaaag cggttaaggc ggggtaatct gctctcgcag tgtttgccgg
    55321 atgcggcgtg aacgccttat ccgacctacg tgtgacgtgt aggcctgata agacgcgata
    55381 agcgtcgcat caggcatggt gctccagacg ccgcaactta ctgctgtgac gcctgctgaa
    55441 acaggttttc ccacatatcg gtcaccagcg cctggtcacg cggcgacaat tcaccggcac
    55501 caatggcttt ttccagactc tggctaacgg tcgtatgcac cgcctgagcg gagtggtcat
    55561 caccactttc cagttctgcg atggctaatg tcaggtggcc acgcaaatac ccactggcaa
    55621 acaactcatc atcactggca tggtcaacca taccgtcgat taatgccaga atgcgtgatt
    55681 caaactccgc gatcatcttc tttcctcatt gtaaaacgtg gcaccggctt cagttgagcg
    55741 cttccggcca cgggaactgt tccgccgtaa gttccggcgt gtgataataa ttttgtagtg
    55801 ctttaatgaa gcgtgccggg cgttcaggaa tgccgtttgc taggtaatcc atcacctgcg
    55861 catgtacccg ccgttgaaac accacacgat ccggttcgaa gtcgccttcc agattgtcgc
    55921 aactgacgtt aaacggatat cccgccgcca cacagaacaa ccagtcgaac gcctgcggct
    55981 tcacttcaac atcttcaaac tggctttgcg tttgggcatc gcgtccgtcc gggcaatacc
    56041 agtagccgaa atcaaccagc tcacggcgcg ctttcccagc aatacaccag tgcgaaatct
    56101 catgaattgc gctggcataa aagccatgag caaagacgat tcggttatac ggtacttccg
    56161 catcagcagg aagatagatc ggttcgtcgt cgcctttaat cagacgggta ttaaattcat
    56221 cggcaaagca gctattaaaa atttcaatca actgctcata gtggtgtgta ctgttcatta
    56281 gttcatcccc aaccagtgga ggatctcctg tccgtggcta tcaaaaagta atttggcact
    56341 catcaccgct gagacaataa caatcatcgg gcggatcagc ttttgtcctt tgctcaacac
    56401 cagtcgtgaa cccatgcgcg cgccgaggaa ctgccccacc agcatgacaa agcccgtcgc
    56461 ccaaatcact ttgccaccga gaataaacag cagtaaaccg ccgatgtttg acgttgcgtt
    56521 gagtaatttg gcgtgcgccg tggctttggc gaggttgaat ccgcacagcg taacaaaggc
    56581 cagtgcgtaa aacgatccgg ctgccggacc aaagaatccg tcataaaaac cgacgcagcc
    56641 accggcaatt aacgcgaagg gtaaaccgta catccgacgc tggcgatctt cttcacccag
    56701 ctttggcatc agcaagaaat agaggccgat acaaattacc agaatgggca aaatctgccg
    56761 taagacatca gcctgaacgt attgcaccag cagtgcgccg ctcattgagc cgacaaaggt
    56821 catggcgata ttgagtttct gatcgcttaa actaaccact ttgcggcgaa taaagtagat
    56881 agtagcggaa atagagccgc cgcaggcttg cagtttattg gttgccagcg cattagcggg
    56941 agacatcccc gctgccatca atgccggaat ggtgagtaac ccaccgccac cggcaatcga
    57001 gtcgataaat cccgccagca tggcgacaaa aaagagaact cccagcaaca gcggggaaac
    57061 cataaacagg ctattaaacg tttccattag atcacgtgct catccagtag cgcctggcag
    57121 gaaggcggca acggaggcgg tgtcttcttc tcaggctttg ttgttcccgg ttttggaggt
    57181 tcaaaccagc tttgcagttc tgccccgcaa ccatcgcctg gtggcggtaa aggttgatct
    57241 tcacactcca gactatcggc aggacaacgt aatcgtacat gcatatgcgc acgatgctgg
    57301 aaccagggtc gcactttgcg caaccagtcg cgatcggtgc ccgcatctag gcaaagttgt
    57361 tgtttaatcg ccggattaac aaaaatgcgc gtgacgtttt tgtcctgggc ggcgagtttg
    57421 atcaagctga aaatttctgg cttccacagc gaggagacaa cgtgtttacc gtcgcgggaa
    57481 actaagtcca gtgcttgcgg gcgcaagagc tgcgcggagg tccagcgagt tttcggcagt
    57541 tgcagaaaga tatcgacatc cagtccggtc tggtggctgg catgaccgcc gttgaaacgc
    57601 ccaccagcag gcatccccat atcgccaatc agcaccgtac ccatgcccag attgctcacc
    57661 tggctactca gacgctggat aaacatcacc agatccgggt gaccgaaata gcgacgctga
    57721 tcggtacgca tgacctgata atgttcggac tgtatcggca gcgtgtcagc gccgacaata
    57781 cagccattag aaaaactgcc tatcgattgt gcgctacccg gcacaggttg ggttattttt
    57841 tgccacggcg tcgctgccag gctagcgcta ctggcaagca gagccagcag cgcaatcgcg
    57901 gttttgttca ttttttacca gcgtggaata tcagtcttca catcggcatt ttgcgcccgc
    57961 tgccgtaaca ggtgatccat taaaacgatc gccagcatcg cttctgcgat cggcactgca
    58021 cggatcccga cacagggatc gtgacggcct ttggtgatca tctcaacttc ttcgccaaag
    58081 cggttaatag tgcgccccgg cacggtaatg ctggaggtcg gtttcagcgc catatgggca
    58141 atgatttgct gcccgctgct gataccgccg agaatgccgc ccgcatggtt gctctggaaa
    58201 ccgtctttgg tgatttcgtc gcggttctgg ctgccgcgca gcgccactac gtcaaaaccg
    58261 tcgccaattt ccacgccttt caccgcgttg atgctcatca gcgcatgggc gatgtcggca
    58321 tccaggcggt caaagaccgg ctcgccaagt ccggcgggga caccactggc aacaacggtg
    58381 actttcgcgc cgatggagtc gccctctttt ttcagcgcgc gcatcagttc atctaacgcg
    58441 tcgattttgt ccgggtccgg gcagaagaac ggattttgtt caaccagcga ccagtctttg
    58501 atttccagcg gaatgtcgcc catctgggtc aagcagccgc ggatttcaat accaaatttc
    58561 tcggcgagat attttttggc aatcgcccct gccgccacgc gcatggcggt ttcgcgggcg
    58621 gaagaacgtc caccgccgcg ataatcgcgc agaccgtatt tttgttcgta ggtgtaatca
    58681 gcatggcctg ggcggaaaac gtctttaatt gcgctgtaat cctgagagcg ctggtcagtg
    58741 ttttcgatca acaagccaat gctggtgcca gtagtaacgc cttcaaaaac accggagaga
    58801 attttgacct gatccggctc gcggcgctgg gtggtatagc gcgatgtccc agggcgacga
    58861 cggtcgaggt catgttgcag gtccgcttcc gtcagcggaa tgcctggcgg aacaccatcg
    58921 acgatgcagc cgagcgccag cccatgcgat tcgccgaagg tggttacgcg aaagagttgt
    58981 ccaattgtgt ttccagccat cacggctccg ttattgttgt gtttgcgtgt ttacttaatc
    59041 tttataaatc gcgaaatatt cacgggccgc cagcagctgc tctttggtga gcataaacac
    59101 accatcgccg ccgttatcaa actccagcca ggtgaacgga acatccggat attgttccat
    59161 aagatgtacc atgctgttgc cgacttcaca aatcagcaca ccatcatcag caaggtaatc
    59221 tgccgcgtta ccgagaatgc gacgcgtcag tttcaggccg tcggtgccag atgccaggcc
    59281 cagttccggc tcgtgacggt attcgtttgg caggtcggac atatcttccg cgtcgacata
    59341 cggcgggtta gtgacaatca gatcgtattg cactttcggc aaatcacgga acagatcgga
    59401 acgaatcgga atgacgttgt ggatcagacc gtgttcttcg atgttctgtt cggcaaccgc
    59461 cagcgcgtcg ggagagatat ctaccgcgtc gacttccgct tccgggaagg cataagcaca
    59521 ggcaatggcg atgcagccgc taccggtaca catatctaaa atatgctgcg gttgcttgct
    59581 gatgagtccg gcaaatttat tgttgatcag ttcaccaatc ggtgagcgcg gcaccagcac
    59641 gcgttcatcg acgtaaaatt catgaccgca gaaccaggct ttgttggtca ggtaagccac
    59701 cggaatgcgc tcgttgacgc ggcggatcac gcgttcaaca atacggtgtt tttcgctgga
    59761 ggtcagacgc gcggtgcgca tatcttccgg gatatccagc ggcaggtaga gcgatggtaa
    59821 caccagctgt acggcttcat cccacgggtt atcggtgccg tgaccgtacc agatatttgc
    59881 cgcgctgaag cggcttaccg accagcgcaa catgtcctga atggtttgca gctcatttac
    59941 tgcttcatca acgaaaattt tatccacgta ttcctccagg gcatgatcgc aataatttcg
    60001 gcggctagtt tgccatgaag atgacgataa atcagcattc acgcgcggtg agtgaggaaa
    60061 aatacgttta aaacgatcga ttgtgctacg agtcgggtaa actgtaggaa aattagaaat
    60121 agagacagat aaatgaaaaa gaaaacaaca cttagcgagg aggaccaggc tctgtttcgc
    60181 cagttgatgg cggggactcg caagattaag caggacacga ttgtccaccg accgcagcgt
    60241 aaaaaaatca gcgaagtgcc ggtgaaacgc ttgatccagg agcaggctga tgccagccat
    60301 tatttctccg atgagtttca gccgttatta aataccgaag gtccggtgaa atatgttcgc
    60361 ccggatgtca gccattttga ggcgaagaaa ctgcgccgcg gcgattattc accggaattg
    60421 tttttggatt tacacggtct gacgcagctg caggccaagc aggaactggg cgcgttgatt
    60481 gccgcctgtc gccgcgaaca tgtgttttgt gcctgcgtga tgcatgggca cgggaagcat
    60541 attttgaagc aacaaacacc gctgtggctg gcacaacatc cgcatgtgat ggcatttcac
    60601 caggcaccga aagagtacgg cggtgatgcg gctttgttgg tgttgattga agtggaagag
    60661 tggttgccgc cggagttgcc ctgaggagtt gagcgtgttg tgccaggagc gcggaatggc
    60721 aggggcgaat gaccctgcca agtgagaaaa ggtgcgctaa tacatattca gtatgtaagt
    60781 gaatcggcag gtagaatcta tgggctgtcg agatgtcgtg tccacgcgta gcttcatatc
    60841 atgcagttgc ctggacgtga tcggttgcgg ctctggcgtc accgtcactt tggcattatc
    60901 acagccggaa gtcgagaagg tcatctgctg tgcgacaaaa cggcagtagg ctccacgctc
    60961 gtcaaaatta ctgccatcac acagtttgcc gttttccatc aggaaagtgg gcagtgtttg
    61021 ctcaataaca ccacctgttg actgcaacct gacgactttg ttatgtacaa cataattttc
    61081 attccaggaa acatcatacg gtgccaggga ctctgtcggg cagtcagagc taacttttgt
    61141 tggtcctata aaaattccgg ccccgaaacg ctgatccata gtactggcgc tttcaacata
    61201 agacgacaca atccagggac aaccgtgtaa atctccatcg taataaacgc caagtactaa
    61261 ttcattacat gaccaggccg ttactggaga gagagcatca tactcttcta atccggggca
    61321 ggtagaatcc ggtaaggttg tattattgcg ctccatcctg agttcagggc cgggaggact
    61381 cccggtccaa ctaactaaag gcaaacttgc tgccgcagtt gaattagatt catacgtgcc
    61441 atattcaact gattgagggg tcaggactga aagatgaaaa cgatactttg caacaacgct
    61501 ggtcgtcccc gcgggtacat agagtgtggc gatctttgtc gcagcctgta tcccagaagc
    61561 gcacagagcc agcaccaata aacatataac atgacgtatt ttatgcattt tatatatact
    61621 caaacgtagc tgtcaggctg gcggtgaaat caccgatggt tacatcacga ccgcttttcg
    61681 cctggatcca ggcccggaaa ttaatatcat tgttgccgtt ggtaagcgca aaagtcgccc
    61741 cggtggtgtt gttaatatcc acccgcttgc cgtcgagcgt ttccatccct atccccacgc
    61801 cctgcgcggt ggaggtgtcg tcgactgcca gaaaacccgg ctgaccgctg tcttccgttc
    61861 cggtgagggt gactttgacg ttgtatcccg ccggcccttt gcagtctttc agcttaaaca
    61921 caaccggcag atgcgcggac tgcccggcga ccatcaaatc ccggttgctg acggtgggaa
    61981 aatgaatttc cgccagattg gcaccatcca ccaccagcgt gcaggattta ctcagcaggt
    62041 tgccgtaaaa atgcaggttg ttatcgactg cccgtgcccc cgtgctggcg gtcagcagca
    62101 ggcctgctaa cagcgcataa ccgggtgaat aaatgtgtgc tgttttcatt ggtaatccac
    62161 ccgtaaggtt gcgtaagcag aaaaattcgt gtccgtcagc accgcatcag ccgctttgac
    62221 cggtaccgcc tccagctttg gcttctggct aaaatcggtg gcgttaatgg aaagcggctc
    62281 gttgagctta aaacgctgac cgtcatgctg aagctcaatt ccgaagcccg gtacatccgt
    62341 ctcaatagca gagtcgttgt aggaggtggc agtccccgtc caggagagtg tcatattcca
    62401 ctcgggatcg cggacatccg agtcgcagct cagttcgtag tccactttct gacgaccata
    62461 attgccatca atgctgtcga taataagatc gcgaaattgc acttcgatgg tttgcccgcc
    62521 gctgatgcta caggcgggcg gcgacatcag cgtaccgtgg aatgtgatgt catcttccgc
    62581 cgccagcaca gccccgcaac cagcgcacag taaaaggagc aggaattttt tcattggtaa
    62641 tccaccacca tggtcatcgt ggcgttaaac tccgacggcg tcagttgcac cccgctctgt
    62701 tttaccggca ccgctttcag ctgaggctga gtgttgacat taaaaggcgt ccagttattt
    62761 tcaccaactg caaacaggga gttgtcggcg gcgttttcta tccgcacccc caggccggcg
    62821 atatccgtgc tgagaaccgt ttctccgttg atcacggcgg tattgccttt gagctgcatc
    62881 cgtaagtcat cgctgacttt attaccgcag ttgagggtgt atttcgcatc ctgttggtaa
    62941 ttggtaccat cgatcctgtt gattatcaca ttgccaaatt tcacagcgct tcccttcacg
    63001 gagcaaggcg gcggtccatc gaccaggatt cgcagcgtca gatccacctg tttgatatca
    63061 ctgccagcaa cagccaaacc gctgataccc catccggcca gcaccagcaa ccctgcgccc
    63121 gctacaaact tgtgactcat aagtccctcc cggcatcagc ctttgcggct ctgctcttca
    63181 tccacctggc agctgttgcc ggtgcatata aagtacagcg gcatccgcgc gccgtagtcg
    63241 ttgatgtaag tcagcaccgg cacgctgtcc atcttcgcat tcagcggtgc ggaggttttc
    63301 ggctccatca tcagcggtgt gaatcccgcc ggcgggttgc cgcctttttt agcgctggcg
    63361 ttgctgatga tcacgtagta cggcgtcggg ttattgacgg tgaagtcctg gccgttacgg
    63421 atgagcgtca ccttatgctg ccacggactt ttcatgctgg tgttctccag cgccttcggt
    63481 cgccagaaca gtttgatacg cgtctgcagc gcaatctgca gtgtgttcgg tttatttgat
    63541 ttcggcggaa tttcgcgcac gttgaaatag aacacgctct cgcggtcggc cggcagtttg
    63601 ttgatatccg gcagcccctg caccttcacc tggccgttca tcatcgcatc gatgcgctgc
    63661 actggcggaa gcaccgcaag cggcgagctg attttgttgc cctttgcatc ctctatccag
    63721 ctctgcgcca gataaggcag tgtgggatcg ttgttgcgca gcgtgacgct gatggacttg
    63781 tcgctttcgt taaacaccag ccgtgtcctg tcgggcgtca ccgaagcgtg gctggagaaa
    63841 ctgaccgctg tcgccagcag cgccagtgcc gggacaacga tttttgcagt gttagtcaga
    63901 ttaaacatgg atcattcctt aatcacgtta ctggtttact gaaattgaag tcggaggttc
    63961 cgttggcgtc acccgctgtg tctgttcctg aatcgccggt ttgaccgccg gcgaagtgat
    64021 aggtgctaca ttgcctttat gctggcatgg cagtaacaag ccgttaaaca gatcggcagg
    64081 cagcgggtcc ggcaggttga tatcgcaatg cgcaacacca ctccagaaca cactcatgtg
    64141 ttcgccaggt ttcaccccag ccagataaac attgccgtca tcatcgacaa ggcccactgt
    64201 ctgctcgtta tcatttttta cttctgcgcc aaacggagga tggctgccgt cgctcaggcg
    64261 cagcaccgcc atcgcttttt gtccgctaat gacagcaaat ttacggtagc caatggcacc
    64321 ttcagttagc gtggcttgca ccaccgactg ggttgcttca gcgttttcag gcaatctgtt
    64381 gaggtcgata tacgcctgat tgcggtaata gttattgacg tcagaaacga cggctttacc
    64441 aaacatattg gtataaacag cagccccgtt accttccacc ggaacatcgg caacgccatc
    64501 ggcatcaatc aacaatcgtg tcccgcccat attctgggta cggtgaagtg cgccaccgtg
    64561 ggcagtaagc gttgcgccgc cctgtaacga caagcccgca gaggtgtatt gcccttcatg
    64621 gtagttcgca ctgaggtcaa cctgcgccag cgaaccatca tggctgtaat agccgtcaac
    64681 gctggtgtgt ttgtcactgg tgccgacgtt caactgatag tgagtcgcgt catcgacacg
    64741 gctgaaataa ccgacctgac tgctgtccgt cccactgcca tagttaccgt tatagctaac
    64801 ggtactgttg tcgccccacg gcatactgag cgaaatgtac atgcctttgt cggcctggtt
    64861 gtcatactcg taacggtagc cagtcatcga gatgctgaca ttacgaatac tgcccatatt
    64921 gaaatagtgc gagagcatga tgttgtagtt tgtctgctcc tcgcgatccc agtaggtatg
    64981 gcgggtatag ttgagataaa ccgaaacacc cgcatcgcgg aagttctggt tataagtggc
    65041 ggtgtacatc tctttgtcgt tgcccgtgcg taccattccg ctgtcactgg catccagata
    65101 ctcgctcatg gtcataaagt tctcttccga gaagcgatat ccagcaaaag taacgcggct
    65161 gttgagctgg tcaaagtctt tggaataact cacacggaag gagttaccgt ccagcgaacc
    65221 tttgccataa gcggtgtctt tatccagttt ggtgtgcgag tgagtaacat caaacgcgac
    65281 cgcgccgaat gtagacaaat cgcgaccgac gccaagcgcc gcagactgat agttttcatc
    65341 tcccagtgcg ccgccatata acgaccagcc gttagcgatc ccccaggaag cttcagcacc
    65401 agaaaaaaat tcaccctcga catggtgtcc ccactcttgc ggacggccca tcatgatctt
    65461 atagcgaacc tgacctgggc gagtgaggta tggcatcgag gcggtgctga tgtcatattc
    65521 ctgcacctgg ccgttctgtt cttcaatgcg aatatgcaac gtaccggaga cagaatcacc
    65581 aagatcctga atacgaaacg gtccagccgg aacctgcgtt tcgtaaatca cacgccccat
    65641 ctggctgacg gtcacttttg ctgtggtgtg tgccacgccg gaaatgtctg gcgcgtagcc
    65701 gcgcagattg ggaggcaaca tttgatcgtc agtactgaca ctgccaccaa catagttaaa
    65761 gccatcaaaa atatcggatc tgaggtaatc ctcgcccagc gccagtttgg cttttaatga
    65821 tggtaacgcc cgccaggcat agtagcgact ccactcccat tttttttgag tttcatcgcc
    65881 gctgaattca tcgtcatcat cattactgcg agtatgttga tagttagtct gccagtcagc
    65941 acgcatacgc cacggcccca ggttaacccc gaccgtcccg ttgccgctga tctcgttact
    66001 atcatcaccg ccattttctt cgtgccgggt ttgtgcgttg atgctgtagt ccgcgacgat
    66061 cccggagatg ccgtcatccc aacgtgaagg cggatcccag tcgggataag tatattcgag
    66121 gtaagcctgt ggcagtgaaa tgactaatgc ggactggctt aaatcagcct taatttccat
    66181 gccttccagt tgaccggatt tcaggcattt agcatcgtgg ctccattgca gatttttcgc
    66241 cacgtcttct tttaaaccaa actgcgctac cagttccggt gtcagacaag catagctttt
    66301 gctcgcgtca tcttcaccag cataccagta aatatcgtac tcttccgcca atggctgttt
    66361 atttagttga acctgtaaat tgtattttcc gggctcaaca tatccctggc tggaaaaacg
    66421 cttcagatca atttttgtgt cgccttttaa ttccagaaaa cgggaatcaa actgaatgtc
    66481 gtcttcagcc cagacactgc tataactccc tgacattgcc agcgcaatgc accacggaag
    66541 aatccgtaat cgaaaaagag aatggtcagg catacacgtt tctatccgtt tttattcttg
    66601 ttcggtcaaa atccatttaa atcccccata gcagtgtcgc tatgggggta tgccttacag
    66661 gtaagtaatc tggaaggtgg tatttgcttc gaaattaccc agatctggcg catcagctgc
    66721 gccgaccagc caggctttaa agttcagtgt ctgcttcgct ttgcctttat tggtagaagt
    66781 gtctttcact attttctggt caatacccat gccgcttttg taagaggtac ccagagagtc
    66841 accgatcgcc aggctaacat tgttaaatgc cgcaccggta tcggtattga aaatggtgta
    66901 ataattgccg ctatttgcag aagaaacggt accagtaaag gtagtggtca ttgtttcctg
    66961 agtatcaaat acgcaatctt gcagacgaat ctggaaactt ttcggcgtgg tggtgccgtt
    67021 atttttcagt ttttcggcac caatatcacc catatcaact tccagtttgt gatcgtccgg
    67081 aacgatagaa cacactgagt cttcaatggt gccgtaaaaa cgtgcagtac cgttgttacc
    67141 cgcagcgatt gttgccgtag aggtgaaaac gcccatcacc attgccgcag caatagctgt
    67201 tttaacaaac ttactcatta tttgtcctta aactataaat aattaaaaat ataaacatta
    67261 caatacattg attcagtcaa tacccaatgt ttatacccag ccattgaata cggcatgcga
    67321 tccaaaaaga acaggtcgta gaaaattgta ttctttggtg tggggccgca tagtgatgtt
    67381 ttcacccccc tgatgcaatt taattttact aaa
//
LOCUS       DAZGTR010000010        66209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGTR010000010 DAZGTR010000000
VERSION     DAZGTR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.010"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(155..634)
                     /gene="folK"
                     /locus_tag="ACHLCY_000760"
     CDS             complement(155..634)
                     /gene="folK"
                     /locus_tag="ACHLCY_000760"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP0368076.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(631..2028)
                     /gene="pcnB"
                     /locus_tag="ACHLCY_000761"
     CDS             complement(631..2028)
                     /gene="pcnB"
                     /locus_tag="ACHLCY_000761"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP0368077.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2088..2984)
                     /gene="gluQRS"
                     /locus_tag="ACHLCY_000762"
     CDS             complement(2088..2984)
                     /gene="gluQRS"
                     /locus_tag="ACHLCY_000762"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP0368078.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(3051..3506)
                     /gene="dksA"
                     /locus_tag="ACHLCY_000763"
     CDS             complement(3051..3506)
                     /gene="dksA"
                     /locus_tag="ACHLCY_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP0368079.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3684..4388)
                     /gene="sfsA"
                     /locus_tag="ACHLCY_000764"
     CDS             complement(3684..4388)
                     /gene="sfsA"
                     /locus_tag="ACHLCY_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP0368080.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4403..4933)
                     /gene="thpR"
                     /locus_tag="ACHLCY_000765"
     CDS             complement(4403..4933)
                     /gene="thpR"
                     /locus_tag="ACHLCY_000765"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP0368081.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5007..7436
                     /gene="hrpB"
                     /locus_tag="ACHLCY_000766"
     CDS             5007..7436
                     /gene="hrpB"
                     /locus_tag="ACHLCY_000766"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP0368082.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPTHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7568..10102
                     /gene="mrcB"
                     /locus_tag="ACHLCY_000767"
     CDS             7568..10102
                     /gene="mrcB"
                     /locus_tag="ACHLCY_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP0368083.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10322..12565
                     /gene="fhuA"
                     /locus_tag="ACHLCY_000768"
     CDS             10322..12565
                     /gene="fhuA"
                     /locus_tag="ACHLCY_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP0368084.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12616..13413
                     /gene="fhuC"
                     /locus_tag="ACHLCY_000769"
     CDS             12616..13413
                     /gene="fhuC"
                     /locus_tag="ACHLCY_000769"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP0368085.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13413..14303
                     /gene="fhuD"
                     /locus_tag="ACHLCY_000770"
     CDS             13413..14303
                     /gene="fhuD"
                     /locus_tag="ACHLCY_000770"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP0368086.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14300..16282
                     /gene="fhuB"
                     /locus_tag="ACHLCY_000771"
     CDS             14300..16282
                     /gene="fhuB"
                     /locus_tag="ACHLCY_000771"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP0368087.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGVCVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16317..17597)
                     /gene="hemL"
                     /locus_tag="ACHLCY_000772"
     CDS             complement(16317..17597)
                     /gene="hemL"
                     /locus_tag="ACHLCY_000772"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP0368088.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17763..17810
                     /gene="yadX"
                     /locus_tag="ACHLCY_000773"
     CDS             17763..17810
                     /gene="yadX"
                     /locus_tag="ACHLCY_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP0368089.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17822..19243
                     /gene="clcA"
                     /locus_tag="ACHLCY_000774"
     CDS             17822..19243
                     /gene="clcA"
                     /locus_tag="ACHLCY_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP0368090.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLFCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAMQLARSKAASASENT"
     gene            19267..19332
                     /gene="yadW"
                     /locus_tag="ACHLCY_000775"
     CDS             19267..19332
                     /gene="yadW"
                     /locus_tag="ACHLCY_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP0368091.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19325..19669
                     /gene="erpA"
                     /locus_tag="ACHLCY_000776"
     CDS             19325..19669
                     /gene="erpA"
                     /locus_tag="ACHLCY_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP0368092.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19716..20339)
                     /gene="yadS"
                     /locus_tag="ACHLCY_000777"
     CDS             complement(19716..20339)
                     /gene="yadS"
                     /locus_tag="ACHLCY_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP0368093.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20377..21177)
                     /gene="btuF"
                     /locus_tag="ACHLCY_000778"
     CDS             complement(20377..21177)
                     /gene="btuF"
                     /locus_tag="ACHLCY_000778"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP0368094.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21170..21868)
                     /gene="mtnN"
                     /locus_tag="ACHLCY_000779"
     CDS             complement(21170..21868)
                     /gene="mtnN"
                     /locus_tag="ACHLCY_000779"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP0368095.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21952..23469
                     /gene="dgt"
                     /locus_tag="ACHLCY_000780"
     CDS             21952..23469
                     /gene="dgt"
                     /locus_tag="ACHLCY_000780"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP0368096.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23599..25023
                     /gene="degP"
                     /locus_tag="ACHLCY_000781"
     CDS             23599..25023
                     /gene="degP"
                     /locus_tag="ACHLCY_000781"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP0368097.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNV
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25178..26335
                     /gene="cdaR"
                     /locus_tag="ACHLCY_000782"
     CDS             25178..26335
                     /gene="cdaR"
                     /locus_tag="ACHLCY_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP0368098.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVVRHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26424..26810)
                     /gene="yaeH"
                     /locus_tag="ACHLCY_000783"
     CDS             complement(26424..26810)
                     /gene="yaeH"
                     /locus_tag="ACHLCY_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP0368099.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVALKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26972..27784)
                     /gene="yaeI"
                     /locus_tag="ACHLCY_000784"
     CDS             complement(26972..27784)
                     /gene="yaeI"
                     /locus_tag="ACHLCY_000784"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFP0368100.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSAGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLVLAHNPDSKEVMRDEPWDLMLCGHTHGGQLHVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27839..28663)
                     /gene="dapD"
                     /locus_tag="ACHLCY_000785"
     CDS             complement(27839..28663)
                     /gene="dapD"
                     /locus_tag="ACHLCY_000785"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP0368101.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28694..31366)
                     /gene="glnD"
                     /locus_tag="ACHLCY_000786"
     CDS             complement(28694..31366)
                     /gene="glnD"
                     /locus_tag="ACHLCY_000786"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP0368102.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSYIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31428..32222)
                     /gene="map"
                     /locus_tag="ACHLCY_000787"
     CDS             complement(31428..32222)
                     /gene="map"
                     /locus_tag="ACHLCY_000787"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP0368103.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32590..33315
                     /gene="rpsB"
                     /locus_tag="ACHLCY_000788"
     CDS             32590..33315
                     /gene="rpsB"
                     /locus_tag="ACHLCY_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP0368104.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33573..34424
                     /gene="tsf"
                     /locus_tag="ACHLCY_000789"
     CDS             33573..34424
                     /gene="tsf"
                     /locus_tag="ACHLCY_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP0368105.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAVLEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34571..35296
                     /gene="pyrH"
                     /locus_tag="ACHLCY_000790"
     CDS             34571..35296
                     /gene="pyrH"
                     /locus_tag="ACHLCY_000790"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP0368106.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35588..36145
                     /gene="frr"
                     /locus_tag="ACHLCY_000791"
     CDS             35588..36145
                     /gene="frr"
                     /locus_tag="ACHLCY_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP0368107.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36237..37433
                     /gene="ispC"
                     /locus_tag="ACHLCY_000792"
     CDS             36237..37433
                     /gene="ispC"
                     /locus_tag="ACHLCY_000792"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP0368108.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDGASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQATTTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37622..38380
                     /gene="ispU"
                     /locus_tag="ACHLCY_000793"
                     /gene_synonym="uppS"
     CDS             37622..38380
                     /gene="ispU"
                     /locus_tag="ACHLCY_000793"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP0368109.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNSGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38393..39250
                     /gene="cdsA"
                     /locus_tag="ACHLCY_000794"
     CDS             38393..39250
                     /gene="cdsA"
                     /locus_tag="ACHLCY_000794"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0368110.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSSHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39262..40614
                     /gene="rseP"
                     /locus_tag="ACHLCY_000795"
     CDS             39262..40614
                     /gene="rseP"
                     /locus_tag="ACHLCY_000795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP0368111.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELLHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40644..43076
                     /gene="bamA"
                     /locus_tag="ACHLCY_000796"
     CDS             40644..43076
                     /gene="bamA"
                     /locus_tag="ACHLCY_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP0368112.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43198..43683
                     /gene="skp"
                     /locus_tag="ACHLCY_000797"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43198..43683
                     /gene="skp"
                     /locus_tag="ACHLCY_000797"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP0368113.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43687..44712
                     /gene="lpxD"
                     /locus_tag="ACHLCY_000798"
     CDS             43687..44712
                     /gene="lpxD"
                     /locus_tag="ACHLCY_000798"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP0368114.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMSMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44817..45272
                     /gene="fabZ"
                     /locus_tag="ACHLCY_000799"
     CDS             44817..45272
                     /gene="fabZ"
                     /locus_tag="ACHLCY_000799"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP0368115.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45276..46064
                     /gene="lpxA"
                     /locus_tag="ACHLCY_000800"
     CDS             45276..46064
                     /gene="lpxA"
                     /locus_tag="ACHLCY_000800"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP0368116.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            46064..47212
                     /gene="lpxB"
                     /locus_tag="ACHLCY_000801"
     CDS             46064..47212
                     /gene="lpxB"
                     /locus_tag="ACHLCY_000801"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP0368117.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYSDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47209..47805
                     /gene="rnhB"
                     /locus_tag="ACHLCY_000802"
     CDS             47209..47805
                     /gene="rnhB"
                     /locus_tag="ACHLCY_000802"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP0368118.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47842..51324
                     /gene="dnaE"
                     /locus_tag="ACHLCY_000803"
     CDS             47842..51324
                     /gene="dnaE"
                     /locus_tag="ACHLCY_000803"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP0368119.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51337..52296
                     /gene="accA"
                     /locus_tag="ACHLCY_000804"
     CDS             51337..52296
                     /gene="accA"
                     /locus_tag="ACHLCY_000804"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP0368120.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52395..54536
                     /gene="ldcC"
                     /locus_tag="ACHLCY_000805"
     CDS             52395..54536
                     /gene="ldcC"
                     /locus_tag="ACHLCY_000805"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP0368121.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54593..54982
                     /gene="yaeR"
                     /locus_tag="ACHLCY_000806"
     CDS             54593..54982
                     /gene="yaeR"
                     /locus_tag="ACHLCY_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0368122.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKVFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            55047..56345
                     /gene="tilS"
                     /locus_tag="ACHLCY_000807"
     CDS             55047..56345
                     /gene="tilS"
                     /locus_tag="ACHLCY_000807"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP0368123.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56394..56654)
                     /gene="rof"
                     /locus_tag="ACHLCY_000808"
     CDS             complement(56394..56654)
                     /gene="rof"
                     /locus_tag="ACHLCY_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP0368124.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56641..56841)
                     /gene="yaeP"
                     /locus_tag="ACHLCY_000809"
     CDS             complement(56641..56841)
                     /gene="yaeP"
                     /locus_tag="ACHLCY_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP0368125.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            57007..57552
                     /gene="yaeQ"
                     /locus_tag="ACHLCY_000810"
     CDS             57007..57552
                     /gene="yaeQ"
                     /locus_tag="ACHLCY_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP0368126.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57549..57971
                     /gene="arfB"
                     /locus_tag="ACHLCY_000811"
     CDS             57549..57971
                     /gene="arfB"
                     /locus_tag="ACHLCY_000811"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP0368127.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLSEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57985..58695
                     /gene="nlpE"
                     /locus_tag="ACHLCY_000812"
                     /gene_synonym="cutF"
     CDS             57985..58695
                     /gene="nlpE"
                     /locus_tag="ACHLCY_000812"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP0368128.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58851..59674)
                     /gene="yaeF"
                     /locus_tag="ACHLCY_000813"
                     /pseudo
     CDS             complement(58851..59674)
                     /gene="yaeF"
                     /locus_tag="ACHLCY_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59728..61446)
                     /gene="proS"
                     /locus_tag="ACHLCY_000814"
     CDS             complement(59728..61446)
                     /gene="proS"
                     /locus_tag="ACHLCY_000814"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP0368129.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIIEYL
                     VKQIKG"
     gene            complement(61558..62265)
                     /gene="tsaA"
                     /locus_tag="ACHLCY_000815"
     CDS             complement(61558..62265)
                     /gene="tsaA"
                     /locus_tag="ACHLCY_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP0368130.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62262..62666)
                     /gene="rcsF"
                     /locus_tag="ACHLCY_000816"
     CDS             complement(62262..62666)
                     /gene="rcsF"
                     /locus_tag="ACHLCY_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP0368131.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62784..63599)
                     /gene="metQ"
                     /locus_tag="ACHLCY_000817"
     CDS             complement(62784..63599)
                     /gene="metQ"
                     /locus_tag="ACHLCY_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP0368132.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63639..64292)
                     /gene="metI"
                     /locus_tag="ACHLCY_000818"
     CDS             complement(63639..64292)
                     /gene="metI"
                     /locus_tag="ACHLCY_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP0368133.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64285..65316)
                     /gene="metN"
                     /locus_tag="ACHLCY_000819"
     CDS             complement(64285..65316)
                     /gene="metN"
                     /locus_tag="ACHLCY_000819"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP0368134.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65504..66079
                     /gene="gmhB"
                     /locus_tag="ACHLCY_000820"
     CDS             65504..66079
                     /gene="gmhB"
                     /locus_tag="ACHLCY_000820"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP0368135.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDTMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15639 a        16485 c        18238 g        15847 t
ORIGIN      
        1 caagtgcaat tggttagggt atggcgatgg gattgatatt tatctgcggg aatttatatt
       61 atgtttctgc aaaaatgttt aaactaaaat ttttattaag gaaagagagg atgtatgatt
      121 ttcagaaaat atttatattc gtaatataaa taaattacca tttgcttaat ttgtcaaatg
      181 ctcttgtatg taagacttca cgcaacgtct ccccatcagg aaacgccagc tccggtgcaa
      241 tctcaaacag cggccacagc ataaatccac gattcttcat atcgtagtgc ggaacggtca
      301 ggcgttcggt atttatcact tcattaccaa acagcatgat atcgagatcc agcgtgcgtg
      361 gtccccagcg ttcagcttta cgaacacgac cctgttgcag ttctatgcgc tgtgtatggt
      421 tgagtaactc ttccggggcg agcgaagttt caagcgccac ggcggcgttg aggtaatcgg
      481 gttgatcttg cggccccagc ggtggggtgc ggtaaaacga agaaacggca agaatgtggc
      541 tttcagggat atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca
      601 gattgctgcc tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg
      661 tttgcgtgga cgacgagtac ggcgacgcgg tgacggctct tcatccaact cgttaagcat
      721 ccctttttga tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgctg
      781 cagttcagcg ttacgttcaa cttcagctcg caaggccaac aggtcataag ccgcacggaa
      841 cttaggatgc tccagcagtt tccatgcgcg tttaccctga cgacgggaca tacgcaactg
      901 caactgccag atatcgcggg ttaacgtcgt cagacgtttc gggattgcca gtgaacggca
      961 ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg tcgtgatagg tcaggccgct
     1021 ttcctgggca atcttctgtg ccgtctccag cagtgggtac cagaacatgg cggcaaacag
     1081 gaacgccggg ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagcacctg
     1141 ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg
     1201 gaacagcggc tggaacagat gatattcaca caacagctta taggtatcgt aaccgtagcc
     1261 cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc ggtgggatat cgttcaacag
     1321 ggtagcgagg cgagggatcg gttctgcggt ttccgggctg atgcgcatac ccaatttggc
     1381 ggcaaaacgt accgcgcgca gcatacgtac cggatcttca cggtagcgcg tttccgggtt
     1441 accaatcaga cggataacgc cgtccttcag atccttcata ccgccaacgt aatcacggac
     1501 ggtaaaatcc gctacgctgt aatagaggct gttgatagtg aaatcgcggc gctgggcgtc
     1561 ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg ccgttttgcc cgcgttggga
     1621 ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg aaggtcgcaa cttcgataat
     1681 ctccgggcca aacattacat gagccagacg gaaacggcga cccaccaggc ggcagttacg
     1741 gaacagtttg cgcacctgct caggcgtggc gttagtggtt acgtcaaaat ctttcggctt
     1801 tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt
     1861 gttgagcctg tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg
     1921 ctgctcacgc gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc
     1981 ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg
     2041 tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca
     2101 tttgagaatg ttgaatttac aattgccgac tcgggcacgg cggtaagccg ccagtttttg
     2161 acggctgact gaaggatttg ctcgacgctg aaatcctgcc agtgtgcttc tgcctgctgc
     2221 cccagaaatt gaagtgccgc gattagtacc gggcgtggat cgcctttcgg caacgcaggc
     2281 gcatgattct gcttggaaag tttagcgcct tgtggattaa gcgccagcgg cagatgaatg
     2341 taatctggca ctttccagcc aaaaagctgg tacagcgaga tttgccttac tgttggttca
     2401 atcagatcag ccccacgtac tatttctgta acgccctgga aatgatcatc aaccacaaca
     2461 gccaggttgt aggcgaacaa cccatcacgg cgatgaatga taaaatcttc ccgtgccagt
     2521 ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa attgcgtgac cggatgctgc
     2581 tggcggatac gcactgcggc attgtctggt ccatgatgca acacccggca atgaccgtcg
     2641 taaataccgc caatgctttg aatacgcgca cgcgtacagg tgcagtaata acttagtcct
     2701 tgttcatgta accaggcgag tgcttcacga taggcgtggt gacgttgcga ttgccagaga
     2761 acatcgccgt cccagtgcag accgtaatgt tccagctggc gcaggatagt ttctgcggca
     2821 ccgggaactt cacgaggcgg gtcgatatct tctatgcgta ccagccagcg accttgccgg
     2881 gcgcgagcct gcaaatagct gccgagcgcg gcgatcagag agccaaaatg aagctcgccg
     2941 gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg tcatctcttt gaacaaaaaa
     3001 taaggcggga gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc
     3061 atctgttttt cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg
     3121 cgcgcttcca gacggcgaat accaatttca acaccgcagg attcgcagta gccgaaatct
     3181 tcgtcttcca cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg
     3241 cggttacgca gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg
     3301 aagttggctg cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga
     3361 ttacgccatg cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct
     3421 tcgcccggct tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat
     3481 gttttacggt tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca
     3541 tgttcggggg aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc
     3601 taaacgtaac acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt
     3661 aaattaacca gttacttact ttactacaat gtaaccggca gtgatttttt aagagccatg
     3721 ccttcagcag aaatttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct
     3781 gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct
     3841 gaatgcagca cggcgaaaaa gataaccgca cgctggcctt cagccgctac gctcatcaac
     3901 tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc
     3961 tccgctaacg taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac
     4021 ataaagtcaa tacggctgcg ttctgcgccg tattttactt cgcttttcag cgagctatag
     4081 cctgacagtt ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa
     4141 agcgtgttga cgcaaataaa tgcgccgctc tggctttgag ttaattccca ggtgtgtggg
     4201 tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa
     4261 ccggtcattg cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc
     4321 acatcggcta aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagaa
     4381 aattccattg cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc
     4441 gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gcatacgacc
     4501 agttaaaacc tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat
     4561 gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gaacggagca
     4621 tattcgccag ctggattaag ccgcgtggcg gctgacgcat ccctaaccac accacacgcg
     4681 aacgcagcca ttgtccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc
     4741 cggctaaaag agaaagcgcc ttctctttct cggcgctcac ttcgcctaaa aatgccagag
     4801 tcagatgcaa attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc
     4861 gccagtggat aatctgttcg cggatttctg caggtaagtc gatagcaaag aacagacgtt
     4921 gcggttcaga catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga
     4981 agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttacctg
     5041 aattacttac cgctctcgat tgtgcgccgc aggtattatt aagtgcgccg accggggccg
     5101 ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta
     5161 tcctgctgga gccgcgtcgt ctggcggcgc gtaacgtcgc gcaacggctg gcggagctgc
     5221 ttaacgaaaa gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc
     5281 cgaatacccg cctggaagta gttaccgaag gcgtgctgac gcggatgatc cagcgtgacc
     5341 cggagttgag cggtgttgga ttggtgatcc tcgatgagtt tcatgagcgc agcttgcagg
     5401 cggatttggc gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac
     5461 tgctgattat gtcggctacg ctggacaacg accgcttgca gcaaatgctg ccagaagcgc
     5521 ccgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg ccgttattta ccactgccca
     5581 cgcatcagcg ttttgacgaa gccgttgcgg tagccaccgc tgaaatgctg cgtcaggaaa
     5641 gtggatcatt actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac
     5701 tggcttcgcg catcggcagc gatgtattgc tctgcccgct gtatggcgcg ttgtcgctga
     5761 acgatcagcg aaaagcgatc ctcccggcac cgcaagggat gcgcaaagtg gtgctggcga
     5821 ccaatattgc tgaaaccagt ttaaccatcg aaggtattcg tctggtggtg gattgtgccc
     5881 aggagcgtgt ggcgcgtttt gatccgcgca cggggcttac gcgactgatt actcaacgcg
     5941 ttagccaggc atccatgacg cagcgtgccg ggcgcgccgg gcgtctggag ccgggtatct
     6001 gcctgcattt aatcgccaag gaacaagcag aacgcgccgc ggcgcaaagt gaaccggaga
     6061 tattacaaag cgatctttcc ggtttgctga tggaattact gcaatgggga tgcagcgatc
     6121 cggcgcagat gagctggctg gatcaaccgc cagtagtgaa tctactggcc gcgaaacgtc
     6181 tgttacaaat gctgggggca ctggagggtg aacggcttag tgcgcaaggg caaaaaatgg
     6241 cagcgctggg taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg
     6301 aagctgctac cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata
     6361 gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg gcagcaacgt agtcagcaac
     6421 tgttaaaacg cttaaacgta cgtggcggtg aggcagacag ttcgcttatc gcgccgctac
     6481 ttgccggggc gtttgccgat cgcattgctc gtcgccgtgg gcaagatgga cgctatcaac
     6541 tggcaaacgg catgggagcg atgctcgatg ccaacgacgc gctaagccgc cacgaatggt
     6601 tgatcgcacc gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgc
     6661 tgccggtcga tattgatgag ttagtacaac gctgcccgca gctggtacag cagtctgaca
     6721 ctgtggagtg ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg caaatcggtc
     6781 tgctgacggt gaaagtgcag ccgctggcga aaccctcgga agacgagttg catcaggcga
     6841 tgcttaatgg catccgcgat aaagatttaa gcgtgctcaa ctggacggcg gaagcggaac
     6901 agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg
     6961 ttgatgatga aagtttattg gcgacgctgg aaacgtggct gctgccacat atgactggcg
     7021 tacattcact acgcggcctg aaatcactcg acatttatca ggcactacgc ggattacttg
     7081 attggggaat gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg
     7141 gaagccggat cgccattcgt tatcatgaag ataacccgcc cgcgctggcg gtgagaatgc
     7201 aagagatgtt tggcgaggcc accaatccga cgatcgccca ggggcgcgtg ccgctggtgc
     7261 tggagttgct ttcacctgcc caaaggccat tacaaatcac acgagatttg agcgccttct
     7321 ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct
     7381 ggccggacga cccggcaaat actgcaccga cgcgacggac gaaaaagtat tcgtaaggtt
     7441 ggttttctcc ctctccctgt gggagagggg accgatcgag cacaaatttt gagagatatc
     7501 ttcttctgtc ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag
     7561 aaaaagcatg gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc
     7621 ggtcaaacaa aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga
     7681 tgactatgag gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa
     7741 gcctcgtggc aaacgcggct ggctatggct gctgttaaaa ctggctatcg tttttgccgt
     7801 gctgatcgcc atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa
     7861 ggtctggcaa ctgcctgcgg cagtttatgg ccgaatggtc aaccttgagc cagatatgac
     7921 catcagcaag aacgagatgg tgaagctgct ggaggcgact cagtatcgtc aggtgtcgaa
     7981 aatgacccgt cctggcgaat ttaccgtgca ggccaacagc attgagatga tccgccgtcc
     8041 gtttgatttc ccggacagta aagaagggca ggtgcgtgcg cgtctgacct ttgatggcga
     8101 tcatctggcg acgatcgtca atatggagaa caaccgtcag ttcggtttct tccgtcttga
     8161 tccgcgtctg atcaccatga tctcctcgcc aaacggtgag cagcgtttgt ttgtgccgcg
     8221 cagtggtttc cctgatttgc tggtggatac tttgctggcg acagaagacc gtcattttta
     8281 cgagcatgac ggcatcagcc tctactccat cggacgtgcg gtgctggcaa acctgaccgc
     8341 cggacgcacg gtgcagggag cgagtacgct gacgcaacag ctggtgaaaa acctgttcct
     8401 ctccagtgag cgttcttact ggcgtaaagc gaacgaagct tacatggcgc tgatcatgga
     8461 cgcgcgttac agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca
     8521 gagcggcgac aacgaaatcc gcggcttccc gctggcgagc ttgtattact ttggtcgccc
     8581 ggtagaagag ctaagcctcg accagcaggc gctgttagtc ggcatggtga aaggagcgtc
     8641 catctacaac ccgtggcgta acccaaaact ggcgctggag cgacgtaatc tggtgctgcg
     8701 tctgctgcaa cagcaacaga ttattgatca agaactctat gacatgttga gtgcacgtcc
     8761 gctgggtgtt cagccacgcg gtggggtgat ctctccacag ccagccttta tgcaactggt
     8821 gcgtcaggag ctgcaggcga aattaggcga taaggtaaaa gatctctccg gcgtgaagat
     8881 cttcactacc tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat
     8941 tccggcactg aagaaacagc gtaagttgag cgatcttgaa actgcgattg tggtcgtcga
     9001 ccgctttagt ggtgaagttc gtgcgatggt cggtggttct gagccgcagt ttgcgggcta
     9061 caaccgtgcg atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct
     9121 gactgcctta agccagccga aaatctatcg tctgaatacg tggattgcgg atgcgccaat
     9181 cgcgctgcgt cagccgaatg gccaggtctg gtcaccgcag aatgatgacc gtcgttatag
     9241 cgaaagcggc agagtgatgc tggtggatgc gttgacccgt tcgatgaacg tgccgacggt
     9301 aaatctgggg atggcgctgg ggctgcctgc ggttacggag acctggatca aactgggcgt
     9361 accgaaagat cagttgcatc cggttccggc gatgctgctg ggggcgttga acttaacgcc
     9421 aatcgaagtg gcgcaggcgt tccagaccat cgccagcggc ggtaaccgtg caccgctttc
     9481 tgcactgcgt tcggtaattg cggaagatgg caaggtgctg tatcagagct tcccgcaggc
     9541 ggaacgcgct gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt
     9601 acaacgcggt acgggtcgtc agcttggggc gaaatacccg aacctgcatc tggcagggaa
     9661 aacagggact accaacaata acgtagatac ctggtttgca ggcattgacg gcagcacggt
     9721 gaccatcacc tgggtaggcc gtgataacaa ccagccgacc aaactgtatg gagccagcgg
     9781 ggcaatgtcg atttatcagc gttatctggc taaccagacg ccaacgccgc tgaatcttgt
     9841 tccgccagaa gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg
     9901 tggcatgcgt gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagcga
     9961 gatgcagcag cagccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc
    10021 gcaacagcaa cctgctcagc aagagcagaa agacagcgac ggtgtagccg gttggatcaa
    10081 ggatatgttt ggtagtaatt aacatctaag cgtgaaatac cggatggcga gttgccatcc
    10141 ggtaaaataa catcccatct aagatattaa ccctttcttt tcatctggtt gtttattaac
    10201 ccttcaggaa cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc
    10261 ttatctttat aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc
    10321 aatggcgcgt tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt
    10381 agccacagcg gtaagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga
    10441 cactatcacc gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac
    10501 tattgcggcg cgacagtctg ctaccggcac taaaaccgat acgccgattc aaaaagtgcc
    10561 acagtctatt tctgttgtga ccgccgaaga gatggcgctg catcagccga agtcggtaaa
    10621 agaagcgctt agctacacgc cgggtgtctc tgttggtacg cgtggcgcat ccaacaccta
    10681 tgaccacctg atcattcgcg gctttgcggc agaaggccaa agccagaata actatctgaa
    10741 tggcctgaag ttgcagggca acttctataa cgatgcggtc attgacccgt atatgctgga
    10801 acgcgctgaa attatgcgtg gcccggtttc cgtgctttac ggtaaaagca gtcctggcgg
    10861 cctgttgaat atggtcagca agcgtccgac caccgaaccg ctgaaagaag ttcagtttaa
    10921 agccggtact gacagcctgt tccagactgg ttttgacttt agcgattcgt tggatgatga
    10981 cggtgtttac tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc agcagaaagg
    11041 gtcagaagag cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg atgataaaac
    11101 caatttcacc ttcctttctt acttccagaa cgagccggaa accggttatt acggctggtt
    11161 gccgaaagag ggaaccgttg agccgctgcc gaacggtaag cgtctgccga cagactttaa
    11221 tgaaggggcg aagaacaaca cctattctcg taatgagaag atggtcggct acagcttcga
    11281 tcacgaattt aacgacacct ttactgtgcg tcagaacctg cgctttgctg aaaacaaaac
    11341 ctcgcaaaac agcgtttatg gttacggcgt ctgctccgat ccggcgaatg cttacagcaa
    11401 acagtgtgcg gcattagcgc cagcggataa aggccattat ctggcacgta aatacgtcgt
    11461 tgatgatgag aagctgcaaa acttctccgt tgatacccag ttgcagagca agtttgccac
    11521 tggcgatatc gaccacaccc tgctgaccgg tgtcgacttt atgcgtatgc gtaatgacat
    11581 caacgcctgg tttggttacg acgactctgt gccactgctc aatctgtaca atccggtgaa
    11641 taccgatttc gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc gcattctgaa
    11701 taaacagaaa caaacgggcg tttatgttca ggatcaggcg cagtgggata aagtgctggt
    11761 caccctaggc ggtcgttatg actgggcaga tcaagaatct cttaaccgcg ttgctgggac
    11821 gaccgataaa cgtgatgaca aacagtttac ctggcgtggt ggtgttaact acctgtttga
    11881 taatggtgta acaccttact tcagctatag cgaatcgttt gaaccttctt cgcaagttgg
    11941 gaaggatggt aatattttcg caccgtctaa aggtaagcag tatgaagtcg gcgtgaaata
    12001 tgtaccggaa gatcgtccga ttgtagttac tggtgccgtg tataatctca ctaaaaccaa
    12061 caacctgatg gcggaccctg agggttcctt cttctcggtt gaaggtggcg agatccgcgc
    12121 acgtggcgta gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg tagtcggttc
    12181 ttatacttac accgatgcgg aatacaccac cgatactacc tataaaggca atacgcctgc
    12241 acaggtgcca aaacacatgg cttcgctgtg ggctgactat accttctttg acggtccgct
    12301 ttcaggtctg acgctgggca ccggtggtcg ttatactggc tccagctatg gtgatccggc
    12361 taactccttt aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt atgatctggc
    12421 gcgagtcggc atggcgggct ccaacgtggc gctgcatgtt aacaacctgt tcgatcgtga
    12481 atacgtcgcc agctgcttta acacttatgg ctgcttctgg ggcgcagaac gtcaggtcgt
    12541 tgcaaccgca accttccgtt tctaatttct cttttggggc acggatttcc gtgcccattt
    12601 cacaagttgg ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt
    12661 aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct
    12721 gccgggaaag tgaccggtct gattggtcac aacggttctg gtaaatccac tctgctcaaa
    12781 atgcttggcc gtcatcagcc tccgtcagaa ggggagattc ttcttgatgc ccagccgctg
    12841 gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt atttgccgca gcagcttcct
    12901 ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc
    12961 gcgctggggc gctttggtgc ggcagatcgc gaaaaggtcg aggaagctat ctcgctggtt
    13021 ggcttaaaac cgctggcgca tcggctggtc gatagtctct ctggcggcga acgtcagcgg
    13081 gcgtggatcg ccatgctggt ggcgcaggat agccgttgtc tgttgctcga cgaaccgact
    13141 tcggcgctgg atatcgccca ccaggttgat gtgctggcgc tggtgcaccg tttaagtcag
    13201 gaacgtggcc tgacggtcat tgccgtgctg cacgatatca atatggcggc acgctactgt
    13261 gattatctgg tcgccctgcg cggtggtgaa atgattgctc agggaacgcc tgcggaaatt
    13321 atgcgcggcg aaaccctcga aatgatttat ggcatcccga tgggtatttt gccgcatccg
    13381 gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc
    13441 gtcgactgtt aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg
    13501 cggcggctat tgaccccaat cgtattgtgg cgctggagtg gttgccggtg gaattactgc
    13561 tggcgctcgg catcgtgcct tacggcgtgg cggataccat caactatcgc ctgtgggtca
    13621 gcgaaccacc attgccggac tcagtgattg acgtcggttt gcgtacagaa cctaaccttg
    13681 aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat ggcccttcac
    13741 cagaaatgct ggcacgtatt gcgccgggtc gcggatttaa cttcagtgac ggcaaacagc
    13801 cgttggcgat ggcgcgtaaa tcgctgacgg aaatggcaga tttacttaac ctgcaaagcg
    13861 cagcagaaac gcatttagcg cactatgaag actttatccg cagcatgaaa ccccgctttg
    13921 tgaagcgtgg tgcacgcccg ttactgctga cgacgcttat cgatccgcgc catatgctgg
    13981 tcttcggtcc aaacagcttg ttccaggaaa ttctcgatga gtacggcatc ccaaatgcct
    14041 ggcaagggga aaccaacttc tggggcagta ccgccgtcag tatcgatcgt ctggcggcgt
    14101 ataaagacgt tgatgtgctc tgttttgatc acgacaacag caaagacatg gatgcgctaa
    14161 tggcaacccc gctgtggcag gccatgccgt ttgtccgtac agggcgcttt cagcgcgtac
    14221 ccgcagtctg gttttatggt gcaacgcttt cggcaatgca ctttgtgcgt gttctggata
    14281 acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt ttcccggcgt tattgctggc
    14341 gctgttagtg attgtcgcta cggcgctaac ctggatgaac ttctcacagg cgctgccgcg
    14401 cagtcagtgg gcgcaggccg cctggtcgcc ggatattgac gtcatcgagc agatgatttt
    14461 tcactacagc ttgttgccgc gtctggcgat ttcgctgctg gtgggcgcag gcctggggct
    14521 ggtgggcgtg ctgtttcagc aagtgctgcg taatccgctg gcggaaccga cgacacttgg
    14581 cgttgctaca ggcgcgcaac tgggaattac cgtcaccaca ctttgggcga tcccaggtgc
    14641 tatggcgagc cagtttgccg cactggtggg cgtttgtgtc gttggcttaa tcgtctttgg
    14701 cgtcgcgtgg gggaaacggc tttcgccggt tacgcttatt ctcgcggggc tggtggtgag
    14761 cctttattgc ggggcaatta atcagctact ggttatcttc catcacgacc agctgcaaag
    14821 catgttcctg tggagcaccg gaacgctgac gcaaaccgac tggggcggcg ttgagcgttt
    14881 atggccgcag ttgctgggcg gcgtgatgtt gacgttattg ctacttcgcc cgttaaccct
    14941 gatggggctt gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc tcgcgcgtct
    15001 ggcagcgctg tcgctggcga ttgtcatcag tgcgctgctg gtgaacgctg tggggattat
    15061 cggctttatc gggttgttcg cgccgctgct ggcaaaaatg ctgggggcgc ggcgtctgct
    15121 gccacgactg atgctggcgt cgctgattgg ggcgctgatc ctctggcttt ccgatcaaat
    15181 catcctctgg ctgactcgcg tgtggatgga agtgtccacc ggttcggtca ctgcgttgat
    15241 cggtgcgccg ctgctactgt ggctgttgcc gcgtttacgc agcattagcg cgccggatat
    15301 gaaggtcaac gatcgtgtcg cggctgaacg ccaacatgtg ctggcgtttg ccctcgcggg
    15361 cggcgtgctg ctgttgatgg ctgtggtggt ggcgctgtcg tttggtcgtg atgcgcacgg
    15421 ctggacgtgg gcgagcgggg cgttgcttga ggatttgatg ccctggcgct ggccgcgaat
    15481 tatggcggcg ctgtttgcgg gcgtcatgct ggcggtggcg ggctgtatta ttcagcggct
    15541 gaccggaaac ccgatggcaa gcccggaagt gctggggatt agctctggcg cagcgtttgg
    15601 cgtggtgttg atgctgtttc tggtgccggg taatgccttt ggctggctgt tacctgcagg
    15661 cagtctcggc gcggcggtga cgctgttgat cattatgatc gccgccggcc gcggtggatt
    15721 ttccccacac cgcatgttac tggcggggat ggcgttaagc accgcgttca ccatgctttt
    15781 gatgatgttg caggcaagtg gtgacccgcg aatggcgcaa gtgctgacct ggatttccgg
    15841 ttcgacctac aacgcgaccg atgcgcaggt ctggcgcacg ggaattgtga tggtgatttt
    15901 gctggcgatt accccgctgt gccgccgctg gctgaccatt ttaccgctgg gtggtgatac
    15961 cgcccgagcc gtaggaatgg cgctgacgcc gacgcgaatt gcgctgctgc tgttagcggc
    16021 ttgcctgacg gcgaccgcga cgatgactat tggaccgttg agttttgttg gtttaatggc
    16081 accgcatatt gcgcggatga tgggctttcg acggacgatg ccacacatcg taatttcggc
    16141 gctggtgggt ggtttactgc tggtgttcgc tgactggtgt gggcggatgg tgctgtttcc
    16201 attccagatc ccggcagggc tgctgtcgac ctttatcggc gcgccatatt ttatctattt
    16261 gttgagaaag cagagccgtt aatagaatgc ctcgcccggt gggcgaggca aaatcatcac
    16321 aacttcgcaa acacccgtcg tgcagcatcg atggtattat tgatatcttc catgctgtgc
    16381 gccacggaca taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc ttcgtccagc
    16441 atcatatgga agaaacgctt aaagcgttcc acgtcacagg ccatcacatc ctgatagcac
    16501 gtcacggact cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg gttaacgacc
    16561 agcggaattc cggcttcttc tgccgcttcc agcagacctt ctgccagacg tgttgtcagc
    16621 tcatccagcg tttcgtgaac gcccggctgc gcgacttcat tcagacaggc gaaacccgct
    16681 gccatcgcaa ttgggttacc ggaaagcgta cccgcctgat agaccggacc cgtcggggcc
    16741 agcgcatcca ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg
    16801 attttgccca ggcaggtgag atccggttcc acaccgtaat aatcctgtgc gccagctagc
    16861 gctacgcgga agccagtcat tacttcatcg atgatcagca atgcgccaaa ttcgtcgcac
    16921 agcgcgcgca gacctggcag gaactctggc agcggtggaa cgcagttcat attgcctgcc
    16981 accggctcga cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca
    17041 gaagccagat cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacaccc
    17101 ggcgagtttg gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca
    17161 gcgtgaccgt ggtaacaacc ttcaaattta atgattttgt cgcgaccggt aaaaccacgg
    17221 gccaggcgga tggcgctcat cgtcgcttca gtgccggagt tcaccatgcg caccatatcc
    17281 atggtcggga ccagttcggt caccagttgc gccattttca cttccatttc ggtcggtgca
    17341 ccaaagctta aaccacgctc ggcggcttca atcacggcat tgcggatagc cggatggtta
    17401 tggcccagca ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca
    17461 tcgtacagat aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta
    17521 aaggcgcgaa caggggagtt cacaccgcca gggatcagct cgcgcgctgc gctgtaaaga
    17581 ttttcagact tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct
    17641 atgttattca taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc
    17701 ctgatttctt agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt
    17761 tcatggaacg tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg
    17821 gatgaaaact gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca
    17881 actgattcgc caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc
    17941 agtcgtcggc acgcttgtcg ggctggcagc ggttgctttt gacaaaggtg tcgcctggtt
    18001 gcagaaccaa cgtatggggg cgctggtaca tactgctgat aattatccgc ttctgttaac
    18061 cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata
    18121 cgcgccggaa gcaggtggtt cggggatccc ggaaattgaa ggggcgctgg aagatcaacg
    18181 tcccgttcgc tggtggcgtg tgttgccggt gaaattcttt ggcgggctgg ggacactcgg
    18241 cggaggcatg gtgctagggc gcgaaggacc aaccgtgcag atcggcggca atatcggtcg
    18301 catggtgctt gatattttcc gcctgaaagg tgatgaagct cgccatacgc tgctggcaac
    18361 cggtgctgct gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat
    18421 tatcgaagag atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat
    18481 tggcgtcatt atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga
    18541 cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat
    18601 tatttttggc attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct
    18661 gcaccgtgtg cacggcggca atattaccaa atgggtactg atgggtggtg cgattggcgg
    18721 tctgtgtgga ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt ttaacctgat
    18781 tcctatcgct accgcgggga atttcagcat gggaatgctg gtgtttattt tcgtcgctcg
    18841 ggtcattacc accttattct gcttctcttc cggcgcgccg ggcggtattt ttgccccgat
    18901 gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg agctgtttcc
    18961 gcaatatcac ctggaggcgg ggacgtttgc tattgccgga atgggggcat tactggcggc
    19021 atctattcgc gcgccgttaa cggggatcat tctggttctg gagatgaccg ataactacca
    19081 gctcattttg ccaatgatta ttaccggtct tggcgcaaca ctattagcgc aatttaccgg
    19141 cgggaaaccg ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg aagctatgca
    19201 actggcgcga agcaaggccg catcagccag cgagaatact tgaacgaaat accagggtat
    19261 tagataatgg cgattattat tgggttagaa tttgcccaat tgccgatgtc gtttggagca
    19321 aaatatgagt gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt
    19381 taaaagcctg atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg
    19441 tggcggttgc agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga
    19501 tatgaccatc gaaaaacagg gcgttggctt ggtggttgat ccgatgagcc tgcaatatct
    19561 ggtcggcggt tccgttgact ataccgaagg actggaaggt tctcgtttca tcgtgaccaa
    19621 cccgaacgcg aaaagcacct gcggttgcgg ttcttccttt agcatctaat ctgttgttca
    19681 tgtgccggat aaactatccg gcacatcttc tctcttcaac gcccattctc atccagcgca
    19741 aacgtcggta gcttaagatg ccaacgaata gccgccagcc gaatcaagag cgtcacgacc
    19801 atgcccatca tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg
    19861 acaataccgc cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatt
    19921 tcgcgggcca gaacatcacg aatgatcccg ccgccaacgc cagtaatgac gcccatacaa
    19981 accgcgatta acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac
    20041 accgccagac caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg
    20101 cgcaccagca cgatggtcag catgctggtt accattgcaa cgaccagatc ggtgggatct
    20161 ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc gaattgtccc gccgcctaca
    20221 gcggtaacca cgcccagtac cagaacacca aaagggtcca tacgcaattt tccggctaac
    20281 aaaacgccgg agatggcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt
    20341 gttcaatccc cactgagcac ctgaaaaggt caggcactaa tctacctgtg aaagcgcatt
    20401 acagagctgt tgtgcagcga ggataatacg tgggcttgca cgttcaaacc agtcactcgt
    20461 gagaggaata acgggaattt tgagctgttc accccagtat tgtttgattt taggaatttg
    20521 gtccggtccg cctgtaatga caatcgcctg tggcgagcgt gctaacacct gttcgcggct
    20581 aacttgcggc cagggaaccc ggctgtcttt aaagatgttt tctccgccac aaacttcgag
    20641 tacctggttc tgaatcgact cttttccact ggtaaatggc ggattaatgc cgaattgcag
    20701 aaaaacacgt tttttaggtt tatcagcata ttgcgctttc aattgcgcgt actgatccag
    20761 cagggattgc gcggcttgtt cggccttgtc tggttgcgga ctccaggggg ccagttgacg
    20821 taacgcattg gcaatttgtt caatgcttgt cgcatcgacc cacatcactt ttattcccag
    20881 cgaagccagc tggtcaacct gccgctcggc attacctcca cgccaggcaa tcaccagatc
    20941 gggtttcagc gcgacaatgc gttccagatt catcccctgc caggtggaaa cctgctcaat
    21001 cttttgcgct tgtggaggat agtcggaata gctgctgacc ccaaccggcg tgatcccggc
    21061 ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag
    21121 ccacagtggc gcaagaaaag acagggcgac cagcgccctg aacagtgact tagccatgtg
    21181 caagtttctg caccagtgac tcaaccatca ggctggactg tttagcggca acagccagga
    21241 actcatcgaa gctaagatga gactgttgat cggccacgtc ggagatggcg cgtacgacaa
    21301 caaacgggac gttgaaattg tggcagacat gggcgattgc cgtcgcttcc atctctacag
    21361 caatggcctg tgggaagttg tggcggattt tcgccagacc aacagaaccg ttgatgaaag
    21421 cgtcgccgct aacaatcagg ccacgtacag cgttaagatt cagttcggca atgcaggcct
    21481 cagcggcagc gatcagttta tcgtcagctt taaagcctgc cggacagcct ggtaactgac
    21541 cgtattcata accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg tccgagacaa
    21601 cgatatcgcc cactttcaac gttggtgcca ggccaccggc agaaccggtg ttaataatca
    21661 catctggctt gcagtgttcc aacagcaaag tggcacccag cgccgcagcg actttaccga
    21721 tgcccgattt cagaagcgca acctcggttc cattcagttg gccggtatag atttcgcaac
    21781 cgccgagact gatagtttga cgtttttcga ttttgtcacg cagcagcgta acttcttctt
    21841 ccattgcacc aatgatgccg attttcatag atttactcgc gataagcccg atttgaaggc
    21901 atagtttacc atgcgcttac ggggaagcgt atttctcacg cgggagagga catggcacag
    21961 attgatttcc gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca gggcgttaaa
    22021 accgaacatg agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat caactctccg
    22081 gcaattcgtc gtctgcaaca aaagacgcag gtttttccac tggagcgcaa tgccgccgtg
    22141 cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg ggcgctacat cgccaaagaa
    22201 attttaagcc gtctgaaaga gcttaaattg ctggaagcat acggcctgga tgaactgacc
    22261 ggaccttttg aaagcattgt tgagatgtca tgcctgatgc acgatatcgg caatccgccg
    22321 tttggtcatt ttggcgaagc agcgataaat gactggtttc gccaacgttt gcacccggaa
    22381 gatgccgaaa gccagcctct gactgacgat cgctgcagcg tggcggcact acgtttacgg
    22441 gacggggaag aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt atgtcatttt
    22501 gaggggaatg cacaaggcat tcgtctggtg catacattga tgcggatgaa tctcacctgg
    22561 gcacaggttg gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg cgaaacgcct
    22621 gagacacatc actatttaat gaaaaagccg ggttattatc tttctgaaga agcctatatt
    22681 gcccggttgc gtaaagaact taatttggcg ctttacagtc gttttccatt aacttggatt
    22741 atggaagctg ccgacgacat ctcctattgt gtggcagacc ttgaagatgc ggtagagaaa
    22801 agaatattta ccgttgagca gctttatcat catttgcacg aagcgtgggg ccagcatgag
    22861 aaaggttcgc tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg ctcaaatagt
    22921 ttaagccgca gtacggaaga tcagtttttt atgtatttac gggtaaacac cctaaataaa
    22981 ctggtaccct acgcggcaca acgatttatt gataatctgc ctgcgatttt cgccggaacg
    23041 tttaatcatg cattattgga agatgccagc gaatgcagcg atcttcttaa gctatataaa
    23101 aatgtcgctg taaaacatgt gtttagccat ccagatgtcg agcagcttga attgcagggc
    23161 tatcgggtca ttagcggatt attagagatt tatcgtcctt tattaagcct gtcgttatca
    23221 gactttactg aactggtaga aaaagaacgg gtgaaacgtt tccctattga atcgcgctta
    23281 ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag taaattaccg
    23341 tcagattctc ctgagtttcc gctatgggaa tattattacc gttgccgcct gctgcaggat
    23401 tatatcagcg gtatgaccga cctctatgcg tgggatgaat accgacgtct gatggccgta
    23461 gaacaataac caggcttttg taaagacgaa caataaattt ttaccttttg cagaaacttt
    23521 agttcggaac ttcgcgctat aaaacgaatc tgaagaacac agcaattttg cgttatctgt
    23581 taatcgagac tgaaatacat gaaaaaaacc acattagcac tgagtgcact ggctctgagt
    23641 ttaggtttgg cgttatctcc gctctctgca acggcggctg agacttcttc agcaacgaca
    23701 gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc
    23761 attaacgtag aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa tttccagcag
    23821 ttcttcggtg atgattctcc gttctgccag gaaggttcac cgttccagag ctctccgttc
    23881 tgccaggggg gccagggcgg taatggcggc ggccagcaac agaaattcat ggcgctgggt
    23941 tccggcgtca tcattgatgc cgataaaggc tatgtcgtca ccaacaacca cgttgttgat
    24001 aacgcgacgg tgattaaagt ccagctgagc gatggtcgta agttcgacgc gaagatggtt
    24061 ggcaaagatc cgcgctctga tatcgcgctg attcagatcc agaacccgaa aaacctgacc
    24121 gcaattaaga tggcggattc tgatgcactg cgcgtgggtg attacaccgt agcgattggt
    24181 aacccgtttg gcctgggaga gacggtaact tccgggattg tctctgcgct ggggcgtagc
    24241 ggcctgaatg tcgaaaacta cgaaaacttc atccagaccg atgcagcgat caaccgtggt
    24301 aactccggtg gtgcgctggt taacctgaac ggcgaactga tcggtatcaa caccgcgatc
    24361 ctcgcaccgg acggcggcaa catcggtatc ggttttgcta tcccgagcaa catggtgaaa
    24421 aacctgacct cgcagatggt ggaatacggc caggtgaaac gcggtgagct gggtattatg
    24481 gggactgagc tgaattccga actggcgaaa gcgatgaaag ttgacgccca gcgcggtgct
    24541 ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag cgggcattaa agcgggtgat
    24601 gtgatcacct cactgaacgg taagccaatc agcagctttg ccgcactgcg tgctcaggtg
    24661 ggtactatgc cggtgggcag caaactgacc ctgggcttgc tgcgcgacgg taagcaggtc
    24721 aacgtgaatc tggaactgca gcagagcagc cagaatcagg ttgattccag ctccatcttc
    24781 aacggcattg aaggcgctga gatgagcaac aaaggcaaag atcagggcgt ggtagtgaac
    24841 aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt
    24901 ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc gtaaagttct tgacagcaaa
    24961 ccgtctgtac tggcactgaa cattcagcgc ggcgacagct ccatctacct gttaatgcag
    25021 taatctccct caaccccttc ctgaaaacgg gaaggggttc tccttacaat ctgtgaactt
    25081 caccacaact ccatacatct tcatcatcct ttaggcattt gcacaatgcc gtacgttacg
    25141 tacttcctta tgctaagccg tgcataacgg aggacttatg gctggctggc atcttgatac
    25201 caaaatggcg caggatatcg tggcacgtac catgcgcatc atcgatacca atatcaacgt
    25261 aatggatgcc cgtgggcgaa ttatcggcag cggcgatcgt gagcgtattg gtgaattgca
    25321 cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc gatatcgatg acgcggtggt
    25381 acgtcatctg cacggtgtgc ggcaagggat taatctaccg ttacggctgg aaggtgaaat
    25441 tgtcggcgta attggcctga caggtgaacc agagaatctg cgtaaatatg gcgaactggt
    25501 ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg atgcacttgt tggctcagga
    25561 tagccgtttg cgggaggaac tggtgatgaa cctgattcag gcagaggaga atactcccgc
    25621 acttactgaa tgggcgcaac ggctggggat cgatctcaat caaccgcgag tggtggctat
    25681 tgttgaggtc gacagcggtc agcttggtgt ggacagcgca atggcggagt tacaacaact
    25741 gcaaaacgcg ctgactacgc ccgagcgtaa taatctggtg gcgattgtct cgctaaccga
    25801 aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag aagatcatcg
    25861 taagcgagtt gaacaactga ttacccgcat gaaagagtac ggccagctgc gttttcgcgt
    25921 ttcactgggc aactatttta ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa
    25981 aacgacgatg gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga
    26041 tctgatgtta cctgtgttac tcgacagttt gcgtggcgac tggcaggcca acgaactggc
    26101 gcgaccgctg gcgcggctga aagcgatgga caataacggc ttgctgcgac gaacgctggc
    26161 ggcgtggttt cgtcacaatg tgcaaccgct ggcaacgtca aaggcgttgt ttattcatcg
    26221 taataccctg gagtatcggc ttaatcgtat atcggaactg accgggcttg atttgggcaa
    26281 ttttgatgac aggttgctgc tgtatgtggc gttacaactg gatgaagagc ggtaggttat
    26341 gcgttaaggt tgaggcggcg ctggctcatt cccctcagcc cgaaggggag aggggaatgc
    26401 ccgaattaac cgtcaattcg ggattattta cgcgttagtt tttccagatc ggcttcaatc
    26461 tcgctgatct tattggttac gactgactcc aggtgacgta agtcgtcgag gatcttacgc
    26521 ttaagagcaa cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac
    26581 cgtagattcg ggctgatttc ctggacttct ttataaccct gacccacacc atcagcgacg
    26641 accgttttac gctgacgcgg atatttaaac ttaacgctct tggcgaaaaa ctcgcctttg
    26701 tctttctgga aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga
    26761 tcaatttctt caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct
    26821 caaaatgagt cagtaaattg tgcttatttt agcatttggc ctggcccgcc cggctcgact
    26881 gtgatcgcag aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta
    26941 cctgctttat tgtggtgata aaattcacgt tctacaccag ttccagcatc gttacttccg
    27001 ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc acgggttgtg tagatgtgtc
    27061 gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc ttcgacaggg gcaaaaggtt
    27121 caccaaccaa cggtacgtgc agttgcccgc cgtgagtatg gccgcacagc atcagatccc
    27181 agggttcatc acgcatgact tctttgctgt cgggattatg cgccagcacc agtcttggca
    27241 gattggcttc gctggcggga ggcggtttgc attgtccggc ccataagtca ccggtgccta
    27301 ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc ctggttaaac aacaccgtga
    27361 tgcccgctga tttcaacgtc tcgccaatta agtgattttt ttctgtacca acagggcgat
    27421 cgtggttgcc gaagcaggca aacgtcggcg cacattcggc aaggggggag aggacatcac
    27481 taaacgccga aaaattcagc ggcatatcaa ataatacgta atcgccgccc agtaatatca
    27541 aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga aatcaggctt aaaggaacaa
    27601 aacgagagta atggagatcg gccagaaaaa gaattttgaa tggtgctgcg ttgtctttaa
    27661 aaaaggcgag gcggtgacgg attaattcaa accagcctgg ctcccagtaa tgcatataac
    27721 cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg caacaatcgg cggcgtgaaa
    27781 tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg ctgcccgtgt gcatactttt
    27841 agtcgatggt acgcagcagt tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt
    27901 tcttaacgat aaccgcacag tagaggctgt atttgccatc ttttgacggc agattacctg
    27961 aaacaaccac agaccccgcc ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt
    28021 aaatacgggt gctctgacca atgtatacgc ccatggaaat gacggaacct tcttcgacaa
    28081 tcaccccttc aaccacttca gagcgtgcgc caatgaagca gttatcttca atgatggttg
    28141 ggttagcctg cagcggttcc agcacgccgc cgatgcccac gccaccggaa aggtggacgt
    28201 ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat
    28261 caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg gcaataaacg
    28321 caccctgacg taccgccgct ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt
    28381 cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta gcggctttct gccccttcga
    28441 tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac cactgatgcg
    28501 tcacccactg accgtcaatt ttttccgcta cacgcagtgc accggaatcc agcagggcga
    28561 tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt ggctggcgtg atctcggcac
    28621 ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa ctcttttcat
    28681 atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac cgctgatgca
    28741 cttcctgctg caactcgtta ttaagtgcac gccggtcagc ggtggcaatt atgaataaat
    28801 cttctactcg ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt cccagatcgg
    28861 caaaaatttt cccgactctc gccagcagtc ctggctggtc gagcgcgata agctcaagga
    28921 acgatttgcg gtcggtatgg gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc
    28981 gtaatttggc gggttggcga cggggctgcg gtggctgcca gctactttgc gtcagtactt
    29041 gctccagacc aaaccgaata acctcatgac gatctgcgga cagcgggctg ccatcgggtt
    29101 ccagcacgat aaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt
    29161 gaacacttaa attgcggcgg tctaattcgg cacagacggc ggcaaacaga taggggcggt
    29221 ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca
    29281 gcggtttgct taaatcatgc tgtaataaat ggcgggcatg ccaggccagt tgatttgggc
    29341 tatggcggac aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt
    29401 cgatgttatc catgcgcagt agtgccagtg cctggagttg gtgatgacga acccgttcgc
    29461 gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggca aaatagagtt
    29521 cgcgcaacag actttgcttc cagctattcc acagcgtttc gttggtggcg caaatgtcag
    29581 ccaccgtcag gcataccaga taacgaagtc ggttttctgt ttgcacttct tcggcaaact
    29641 gcttgatgac ttccgggtcc tgaatatcgc ggcgttgggc ggtcaccgac atcaacaggt
    29701 gctggcgaac cagccaggca accagctgtg tttcgcgcga gttaagcccg tggagttcgg
    29761 caaaatgcac tacatcctga gcaccgagaa tggagtggtc gccgccgcgt cctttggcga
    29821 tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggcaggcgc ggccagacgt
    29881 ccacacacaa cggatggcgc tggcgcgttt cttcactggc aaaactctcc agtttcagca
    29941 tcacgcggat agtatgttca tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc
    30001 cgacgatata cgaccattgc ggcatatacg cgccgagcac gctatggcga tgcattggca
    30061 atagcccgcg ccgcaccgct ccggggtgac gcagaatgct caaaaacagt tttcgtgctt
    30121 ccggaatatt acacagcggt tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg
    30181 tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga
    30241 tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgattagc gtaccgcgta
    30301 gctgaaactc atcgtcgagt ggacgtggtt tttcgtcggc gggaagggcg aggatggctt
    30361 catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgta acgcggaagt
    30421 aatccttcat catccgctcg accggttcgt tgccttcacc actgtaattc agacgctgag
    30481 cgacgctaag ttggcgatcg aataacagac gattatcgta acggctgacg accagatgca
    30541 gggcaaagcg aatacgccac agtatatgca gacattcgtt taattccgcc cgctccgctg
    30601 aggttaagaa gccaaaccca accatttcat ccagcgatgt tgcgccaaaa tgacggcggg
    30661 ccacccattg cagagtgtgg atatcgcgca agccgccagg gctacttttg atgtctggtt
    30721 caagattgta gctggtgccg tggtaacgct gatggcgctg gttctgttct tcaactttcg
    30781 ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt tgcagttcga
    30841 ggaagagcgc aacatcgcca attaataagc gggattcgat taaattggtg gcgacggtta
    30901 aatccgataa cccttccagc atgcactctt caagcgtgcg cacactatga ccgacttcca
    30961 gctttacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga tcgtccggga
    31021 gctttttacg gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc tcgccacgac
    31081 cgtagccacc gacggcgacc aatgccaggt cggcaatctg gctgaatccc gcttcaatcc
    31141 ataatcgttg caggagctgg tcgataaact cggtgcgcgc ctcaatcaac tgttctgcag
    31201 agatcccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata tgggctttta
    31261 tcccaccgac ggttaattca tcacggggcc agacgcatgg attttgcggt tgaccgggca
    31321 gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg cgccacccat
    31381 aagattaaat tatcgcatta aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc
    31441 gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca gccgttatca
    31501 gtcaccacaa tagtatgctc atattgtgca gacaagctgc gatctttggt ttttaccgtc
    31561 cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg ctcgatggtg
    31621 aacgtcatcc caggtttcag tacgacgttg gtttcacggg agtcatagtg cagcacctgc
    31681 ggttcttcat ggaagccgcg accaataccg tgtccgcaat attcacgaac gacggagaag
    31741 ccttctgctt cgacaaattt ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt
    31801 ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga tgcggcacag acgttcgccc
    31861 atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa accatctttg
    31921 attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttcgc atcgtccggg
    31981 ataccgtggc acaccacttc attaatagag atgcaaacgg atttcggata gccgtgatag
    32041 ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt aatcattaca gatgcgatcc
    32101 agctcgccgg tgctgacgcc cggtttaaca tacggttcga tcatctccag cacttcggca
    32161 gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat tgagatagcc
    32221 attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg
    32281 tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc actgaacgtt
    32341 ttctataata gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt attggtgtcc
    32401 atgacgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat acacagactg
    32461 gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcacg tattccgggg
    32521 tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt ttatatagag
    32581 gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt tcacttcggt
    32641 caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa
    32701 gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct ggctgaactg
    32761 aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc
    32821 gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca tcgctggctg
    32881 ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg
    32941 gaaactcagt ctcaggacgg tactttcgaa aagctgacca agaaagaagc gctgatgcgc
    33001 actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat gggcggtctg
    33061 ccggacgctc tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa agaagcaaac
    33121 aacctgggta ttccggtatt tgctatcgtt gataccaact ctgatccgga cggtgttgac
    33181 ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt
    33241 gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga agaaagcttc
    33301 gtagaagctg agtaataagg cttgataact cccccaaaat agttcgagtt gcagaaaggc
    33361 ggcaagctcg agaattcccg ggagcttaca tcagtaagtg accgggatga gcgagcgaag
    33421 acaacgcatc tgcggcgcga aatatgaagg gggagagccc ttatagacca ggtagtacac
    33481 gtttggttag ggggcctgca tatggccccc tttttcactt ttatatctgt gcggtttaat
    33541 gccgggcaga tcacatctcc gaggatttta gaatggctga aattaccgca tccctggtaa
    33601 aagagctgcg tgagcgtact ggcgcaggca tgatggattg caaaaaagca ctgactgaag
    33661 ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag
    33721 cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact
    33781 acggcatcat tctggaagtt aactgccaga ctgacttcgt tgcaaaagac gctggtttcc
    33841 aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg caaaatcact gacgttgaag
    33901 ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt ggtgagaaca
    33961 tcaacattcg ccgcgttgct gtgctggaag gcgacgttct gggttcttat cagcacggtg
    34021 cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga agagctggtt aaacacatcg
    34081 ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc agaagacgta tccgctgaag
    34141 tggtagaaaa agaataccag gtacagctgg atatcgcgat gcagtctggt aagccgaaag
    34201 aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa gtttctctga
    34261 ccggtcagcc gttcgttatg gaaccaagca aaactgttgg tcagctgctg aaagagcata
    34321 acgctgaagt gactggcttc atccgcttcg aagtgggtga aggcatcgag aaagttgaga
    34381 ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc
    34441 gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc agctaaccct tgtggggctg
    34501 cgctgaaaag cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt gacagtctca
    34561 ggaaagaaac atggctacca atgcaaaacc cgtctataaa cgcattctgc ttaagttgag
    34621 tggcgaagct ctgcagggca ctgaaggctt cggtattgat gcaagcatac tggatcgtat
    34681 ggctcaggaa atcaaagaac tggttgaact gggtattcag gttggtgtgg tgattggtgg
    34741 gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga
    34801 ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg cactgcaccg
    34861 cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt gcgacagcta
    34921 cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg
    34981 tacaggtaac ccgttcttta ccaccgactc agcagcttgc ctgcgtggta tcgaaattga
    35041 agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg tttaccgctg atccggcgaa
    35101 agatccaacc gcaaccatgt acgagcaact gacttacagc gaagtgctgg aaaaagagct
    35161 gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac cataaattac cgattcgtgt
    35221 tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac
    35281 tttaatcacg gaataattcc cgtgatggat aaataagggt aagattccgc gtaagtatcg
    35341 cgggggcgtt agtctggtta taaggcgtta ttgttgcagg cagtttggtc acggccagcg
    35401 cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga gcactgctcg gggccaaaat
    35461 ggcaaataaa atagcctaat aatccagacg attacccgta atatgtttaa tcagggctat
    35521 acttagcaca cttccactgt gtgtgactgt ctggtctgac tgagacaagt tttcaaggat
    35581 tcgtaacgtg attagcgata tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga
    35641 agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt gcttctccca gcctgctgga
    35701 tggcattgtc gtggaatatt acggcacgcc gacgccgctg cgtcagctgg caagcgtaac
    35761 ggtagaagat tcccgtacac tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt
    35821 tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg aactctgcgg gtagcgacat
    35881 ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg
    35941 tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg cgtcgtgacg cgaacgacaa
    36001 agtgaaagca ctgttgaaag ataaagagat cagcgaagac gacgatcgcc gttctcagga
    36061 cgatgtacag aaactgactg atgctgcaat caagaaaatt gaagcggcgc tggcagacaa
    36121 agaagcagaa ctgatgcagt tctgatttct tgaacgacaa aaacgccgct cagtagatcc
    36181 ttgcggatcg gctggcggcg ttttgctttt tattctgtct caactctgga tgtttcatga
    36241 agcaactcac cattctgggc tcgaccggct cgattggttg cagcacgctg gacgtggtgc
    36301 gccataatcc cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca
    36361 tggtagaaca gtgcctggaa ttctctcccc gctatgccgt aatggacgat ggagcgagtg
    36421 cgaaacttct taaaacgatg ctacagcaac agggtagccg caccgaagtc ttaagtgggc
    36481 aacaagccgc ttgcgatatg gcagcgcttg aggatgttga tcaggtgatg gcagccattg
    36541 ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa accattttgc
    36601 tggccaataa agaatcactg gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga
    36661 gcaaagcgca attgttaccg gtcgatagcg aacataacgc catttttcag agtttaccgc
    36721 aacctatcca gcataatctg ggatacgctg accttgagca aaatggcgtg gtgtccattt
    36781 tacttaccgg gtctggtggc cctttccgtg agacgccatt gcgcgatttg gcaacaatga
    36841 cgccggatca agcctgccgt catccgaact ggtcgatggg gcgtaaaatt tctgtcgatt
    36901 cggctaccat gatgaacaaa ggtctggaat acattgaagc gcgttggctg tttaacgcca
    36961 gcgccagcca gatggaagtg ctgattcacc cgcagtcagt gattcactca atggtgcgct
    37021 atcaggacgg cagtgttctg gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc
    37081 acaccatggc atggccgaat cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac
    37141 taagtgcgtt gacatttgcc gcaccggatt atgatcgtta tccatgcctg aaactggcga
    37201 tggaggcgtt cgaacaaggt caggcaacga cgacagcatt gaatgccgca aacgaaatca
    37261 ccgttgctgc ttttcttgcg caacaaatcc gctttacgga tatcgctgcg ttgaatttat
    37321 ccgtactgga aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg
    37381 atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg tctcgcaagc tgaggataat
    37441 ccggctacag agagtcgcgc tatttgttag cgtagggctt cagtgatata gtctgcgcca
    37501 tctgatcgta agtagttggc tttataaggt caggtatgcc gtggttttac acggcttttt
    37561 tttgtatagg cttcagtatt cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt
    37621 gatgttgtct gctactcaac cacttagcga aaaattgcca gcgcatggct gccgtcatgt
    37681 tgcgatcatt atggacggca atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt
    37741 tgggcataaa gccggggcaa aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg
    37801 tattgaggcg ttaacgctgt atgcctttag tagtgaaaac tggaaccgac cagcgcagga
    37861 agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca
    37921 ccgacataac gtgcgtctgc gtattattgg cgataccagt cgctttaact cgcgtttgca
    37981 agaacgtatt cgtaaatctg aagcgctaac agccgggaat agcggtctga cgctgaatat
    38041 tgcggcgaac tacggtggac gttgggatat agtccaggga gtcaggcaac tggctgaaaa
    38101 ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa gagatgctaa accagcatgt
    38161 ctgtatgcat gaactggccc ctgtagattt agtaattagg actggggggg agcatcgcat
    38221 tagtaacttt ttgctttggc aaattgccta tgccgaactt tactttacag atgttctctg
    38281 gcccgatttc gatgaacaag actttgaagg ggcgttaaat gcctttgcta atcgagagcg
    38341 tcgtttcggc ggcaccgagc ccggtgatga aacagcctga tgggggtcgc ttttgctgaa
    38401 gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt
    38461 gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc atgctggcag cgtgggaatg
    38521 gggacagctt agcggtttta ccactcgttc gcagcgagta tggttggcgg tgttatgcgg
    38581 gttattgttg gcgctgatgc tttttctgtt gccggaatat caccgaaata ttcatcaacc
    38641 gctggttgaa atctcacttt gggcttcgtt gggttggtgg attgtcgcgc tattgctggt
    38701 gctgttttac ccaggttccg cagcaatctg gcgtaactct aaaacattgc gccttatttt
    38761 tggcgtgcta accattgttc ccttcttctg gggcatgctg gcgttacggg cctggcacta
    38821 cgacgagaat cattacagtg gcgcaatatg gctgctctat gtcatgatcc tggtatgggg
    38881 cgctgactct ggcgcatata tgtttggcaa attgtttggt aaacataagc tggcaccgaa
    38941 ggtttctccg ggtaaaacct ggcaaggctt tataggtgga ctagcgaccg cagcggtaat
    39001 ctcatggggt tatggcatgt gggcgaatct cgacgtcgct cccgtcacct tactcatttg
    39061 ctctattgtc gcagcgttag cctcagtgct cggcgatctg accgagagta tgtttaagcg
    39121 tgaagcagga attaaggaca gcagtcattt aattccagga cacggtggta ttttagatcg
    39181 tattgatagc ctgacggctg cggtaccggt ctttgcttgc ttgttgttac tggtattcag
    39241 gacgctttaa cggaaggtaa tatgctgagt tttctctggg atttggcttc gttcatcgtt
    39301 gcactgggtg tacttatcac cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt
    39361 ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg cgctctggcg gcgaactgat
    39421 aagctcggca ccgaatatgt tatcgccctg atcccgctgg gcggttatgt caaaatgctg
    39481 gatgagcgcg cagaaccggt cgttccggaa ctcctccacc atgccttcaa taataaatct
    39541 gtcggccaac gagcggcgat tattgccgca ggtccggttg caaacttcat ttttgctatc
    39601 tttgcctact ggctggtttt tattattggt gtgcctggcg tacgtccggt ggttggtgaa
    39661 atagcagcca attcgatagc tgcggaagca caaattgcac caggtacgga actaaaagcc
    39721 gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt tgcagttggt cgataaaatt
    39781 ggcgatgaaa gcaccaccat tacagtagcg ccatttggca gcgaccaacg gcgggatgta
    39841 aagctcgatt tacgtcactg ggcgtttgag ccggataaag aagatccggt atcttcgctg
    39901 gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg aaaatgtgca accaaactcg
    39961 gcggcaagca aggcaggttt gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc
    40021 ttaacgcagt gggtgacctt tgtgatgctt gtccgggata acccgggtaa atccttagcg
    40081 ttagaaatcg aaaggcaggg aagtcccttg tctttgacat taatcccgga gagtaaaccg
    40141 ggtaatggta aagcgattgg ttttgtcggt attgagccga aagtcattcc tttgccagat
    40201 gagtataaag ttgtacgcca gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa
    40261 acgtggcagc tgatgaagct gacggtcagt atgctgggaa aattgatcac cggtgatgtg
    40321 aaactgaaca acctcagtgg gccgatctct atcgccaagg gggctgggat gacagcggaa
    40381 ctcggggtag tttattacct gccgtttctt gcgcttatta gcgtgaactt agggataatt
    40441 aacctgtttc cgttgcccgt acttgacggg gggcatctgc tgttccttgc gatcgaaaag
    40501 atcaagggcg gaccggtatc cgagcgggtt caagactttt gttatcgcat tggctcgatt
    40561 ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt tctctcggtt atgagagtta
    40621 gttaggaaga acgcataata acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt
    40681 ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt gaaagatatt catttcgaag
    40741 gccttcagcg tgtcgccgtt ggtgcggccc tcctcagtat gccggtgcgc acaggcgaca
    40801 cggttaatga tgaagatatc agtaatacca ttcgcgctct gtttgctacc ggcaactttg
    40861 aggatgttcg cgtccttcgt gatggtgata cccttctggt tcaggtaaaa gaacgtccga
    40921 ccattgccag cattactttc tccggtaaca aatcggtgaa agatgacatg ctgaagcaaa
    40981 acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga tcgcaccacc attgccgata
    41041 tcgagaaagg tctggaagac ttctactaca gcgtcggtaa atatagcgcc agcgtaaaag
    41101 ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa actggtgttc caggaaggtg
    41161 tgtcagctga aatccagcaa attaacattg ttggtaacca tgctttcacc accgacgaac
    41221 tgatctctca tttccaactg cgtgacgaag tgccgtggtg gaacgtggta ggcgatcgta
    41281 aataccagaa acagaaactg gcgggcgacc ttgaaaccct gcgcagctac tatctggatc
    41341 gcggttatgc ccgtttcaac atcgactcta cccaggtcag tctgacgcca gataaaaaag
    41401 gtatttacgt cacggtgaac attaccgaag gcgatcagta caagctttct ggcgttgaag
    41461 tgagcggcaa ccttgccggg cactccgctg aaattgagca gctgactaag atcgagccgg
    41521 gcgagctgta taacggcacc aaagtgacca agatggaaga tgacatcaaa aagcttctcg
    41581 gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca
    41641 aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc
    41701 gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg
    41761 aaggtgcatg gctgggaagc gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg
    41821 gcttctttga aactgtcgat accgataccc aacgtgttcc gggtagcccg gaccaggtag
    41881 atgtcgtcta caaggtaaaa gagcgcaaca ccggtagctt caacttcggg attggttacg
    41941 gtactgaaag tggcgtgagc ttccaggctg gtgtgcagca ggataactgg ttaggtacag
    42001 gttatgctgt tggtatcaac gggaccaaaa acgattacca gacctatgct gaactgtcgg
    42061 taaccaaccc gtacttcact gtagatggtg taagcctcgg tggtcgtctc ttctataatg
    42121 acttccaggc agatgacgcc gacctgtccg actataccaa caagagttat ggtacagacg
    42181 tgacgttggg cttcccgatc aacgaatata actcgctgcg tgcaggtctg ggttatgtac
    42241 ataactccct gtccaacatg cagcctcagg ttgcgatgtg gcgttatctg tactctatgg
    42301 gtgaacatcc gagcacctct gatcaggata atagcttcaa aacggacgac ttcacgttca
    42361 actatggttg gacctataac aagcttgacc gtggttactt cccgacagat ggttcacgtg
    42421 tcaacctgac cggtaaagtg accattcctg gatcggataa cgaatactac aaagtgacgt
    42481 tagacacggc gacttatgtg ccgatcgatg acgatcacaa atgggttgtt ctggggcgta
    42541 cccgctgggg ttatggtgat ggtttaggcg gcaaagagat gccgttctac gagaacttct
    42601 atgccggtgg ttccagcacc gtgcgtggct tccagtccaa tacaattggt ccgaaagcag
    42661 tttacttccc gcatcaggcc agtaattatg atccggacta tgattacgaa tgtgcgactc
    42721 aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg
    42781 ttgccagcct cgagttcatc accccgacgc cgtttattag cgataagtat gctaactcgg
    42841 ttcgtacttc cttcttctgg gatatgggta ccgtttggga tacaaactgg gattccagcc
    42901 aatattctgg atatccggac tatagtgatc caagcaatat ccgtatgtct gcgggtatcg
    42961 cattacaatg gatgtcccca ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa
    43021 agtacgatgg agacaaggca gaacagttcc agtttaacat cggtaaaacc tggtaagtgt
    43081 tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt aggcgatcaa tataagatcg
    43141 ccgggccacg caaagaactg caccctccgg tgcaaatggg atggtaagga gtttattgtg
    43201 aaaaagtggt tattagcggc aggtctcggt ttagcactgg caacttctgc tcaggcggct
    43261 gacaaaattg caatcgtcaa catgggcagc ctgttccagc aggtagcgca gaaaaccggt
    43321 gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa
    43381 accgatctgc aggctaaaat gaaaaagctg cagtccatga aagcgggcag cgatcgcact
    43441 aagctggaaa aagacgtgat ggctcagcgc cagacttttg ctcagaaagc gcaggctttt
    43501 gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca aactggttac tcgtatccag
    43561 actgctgtga aatccgttgc caacagccag gatatcgatc tggttgttga tgcaaacgcc
    43621 gttgcttaca acagcagcga tgtaaaagac atcactgccg acgtactgaa acaggttaaa
    43681 taagtaatgc cttcaattcg actggctgat ttagcgcagc agttggatgc agaactacac
    43741 ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc aatctgcaca aacaggtcac
    43801 attacgttca tggttaaccc aaaataccgt gagcatttag gcttgtgcca ggcgtccgcg
    43861 gttgtcatga cccaggacga tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat
    43921 ccctacctga cttacgcgcg catggcgcaa attttagata ccacgccgca gcccgcgcag
    43981 aacattgcac ccagtgcggt gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt
    44041 ggcgctaacg cggtgattga gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc
    44101 ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta
    44161 accatttacc atgagatcca gatcggtcag aattgcctga tccagtccgg aacagtggta
    44221 ggcgcagacg gctttggtta tgccaacgat cgtggtaact gggtgaagat cccacagatt
    44281 ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc
    44341 gcgctggatg acactattat tggcaatggc gtgatcattg ataaccagtg ccagattgca
    44401 cataacgtcg tgattggcga caatacggcg gttgccggtg gcgtcattat ggcgggcagc
    44461 ctgaaaattg gtcgttactg catgatcggc ggagccagcg taatcaacgg gcatatggaa
    44521 atatgcgaca aagtgacggt tacgggcatg agtatggtga tgcgtcccat cactgaacca
    44581 ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca
    44641 ctggtgatga acattgatga catgagcaag cgtctgaaat cgcttgagcg caaggttaat
    44701 caacaagact aacgttccat cttttgttcg ccaaacttta cggcctgtct cattcttacg
    44761 attgcggcag gccgtgttat tattgtcgtt tcttatattt tgacaggaag agtatcttga
    44821 ctactaacac tcatactctg cagattgaag agattttaga acttctgccg caccgtttcc
    44881 cgttcttact ggtggatcgc gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa
    44941 aaaatgtctc tgtcaatgag ccattcttcc agggccattt ccctggaaaa ccgattttcc
    45001 cgggtgtgct gattctggaa gcaatggcac aggcaacagg tattctggcg tttaaaagcg
    45061 taggaaaact ggaaccgggt gagctgtact acttcgctgg tattgacgaa gcgcgcttca
    45121 agcgcccggt cgtgcctggc gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc
    45181 gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa
    45241 cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat tgataaatcc gcctttgtgc
    45301 atccaaccgc cattgtggaa gagggcgcgt caattggcgc gaacgcacac attggtcctt
    45361 tttgtatcgt tggaccccat gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg
    45421 tcgtgaatgg tcatactaaa attggccgcg ataatgagat ttatcagttc gcctccatcg
    45481 gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc
    45541 gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac agtccagggc ggtggattga
    45601 cgaaggtggg cagcgacaac ttactgatga tcaacgcgca cattgcgcac gattgtacgg
    45661 taggtaaccg ctgtattctc gccaacaacg caacgctggc gggtcacgta tcggttgacg
    45721 acttcgcgat catcggcggc atgaccgcag tccatcagtt ctgcatcatt ggtgcgcacg
    45781 tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg
    45841 gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc
    45901 gtgaggcgat taccgctatc cgcaatgcgt ataagctgat ttatcgtagc ggtaaaacgc
    45961 tcgatgaagt gaaaccggaa attgctgaac tggcggaaac atatccggaa gtgaaagcct
    46021 ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg ttaatgactg aacagcgtcc
    46081 attaacgatt gccctggtcg ccggagaaac ctccggcgat atcctggggg ccggtttaat
    46141 ccgcgctctg aaagaacgtg tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat
    46201 gcaggctgaa ggctgcgaag cctggtacga aatggaagaa ctggcggtga tgggcattgt
    46261 tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt gccgatctga caaagcgttt
    46321 tggcgaactg aagccagata tttttgttgg tattgatgcg cctgacttca atattactct
    46381 tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat tacgtcagtc catccgtctg
    46441 ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc accgatctgg tgctcgcatt
    46501 tctgcctttc gaaaaagcgt tttatgacaa atacaatgta ccgtgccgct ttatcggtca
    46561 taccatggct gatgccatgc cgttagatcc agataaaaat gccgcccgtg atgtgctggg
    46621 gatccctcac gatgcccact gcctggcgtt gctaccaggg agccgtggtg cagaagttga
    46681 aatgcttagt gccgatttcc tgaaaacggc ccagcttttg cgccagacat attcggatct
    46741 cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc
    46801 tgaagtcgcg ccagaccttt cagttcattt gctggatggg atgggccgtg aggcgatggt
    46861 cgccagcgat gcggcgctac tggcgtcggg tacggcagcc ctggagtgta tgctggcgaa
    46921 atgcccgatg gtggtgggat atcgcatgaa gccttttacc ttctggttgg cgaagcggct
    46981 ggtgaaaact gattatgtct cgctgccaaa tctgctggcg ggtagagagt tagtcaaaga
    47041 attattgcag gaagagtgtg agccgcaaaa actggctgcg gcgctgttac cgctgttggc
    47101 gaacgggaaa accagccacg cgatgcacga taccttccgt gaactgcatc agcagatccg
    47161 ctgcaatgcc gatgagcagg cggcacaagc cgttctggag ttagcacaat gatcgaattt
    47221 gtttatccgc acacgcagct ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt
    47281 ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc gcccgattgc cgggctgaat
    47341 gattccaaaa agctgagcga aaaacgccgt ctggcgctct atgaagagat caaagagaaa
    47401 gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt
    47461 catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat
    47521 gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa
    47581 ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc tggcgaaagt gacgcgtgac
    47641 gccgaaatgg cggcgctgga tattgttttc ccgcaatatg gttttgccca acacaaaggg
    47701 tacccaaccg cttttcatct ggaaaaactg gctgaacacg gcgcgaccga acaccatcgg
    47761 cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt cctgattctt gtgtcgagat
    47821 taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc
    47881 gactactcga tgatcgatgg cctggccaaa accgcaccgt tggtaaaaaa ggcggcggcg
    47941 ttgggtatgc cagcactggc gatcaccgat ttcaccaacc tttgtggtct ggtgaagttc
    48001 tacggagcgg gacatggcgc agggattaag cctatcgtcg gggcagattt taacgtccag
    48061 tgcgacctgc tgggtgatga gttaacccac ctgacggtac tggcggcgaa caataccggc
    48121 tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg
    48181 ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag ggttgatcct tctttccggc
    48241 ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta acagcgcgct ggtagatgag
    48301 tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt ttctcgagct gatccgcact
    48361 ggcaggccgg atgaagaaag ctatctgcac gcggcggtgg aactggcgga agcgcgcggt
    48421 ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca gcagcgactt tgacgcacac
    48481 gaaatccgcg tcgcgatcca cgacggcttt accctcgacg atcctaaacg cccgcgtaac
    48541 tattcgccgc agcaatatat gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc
    48601 cccgaagccc ttgccaacac cgttgagatc gccaaacgct gtaacgtaac cgtgcgtctt
    48661 ggtgaatact tcctgccgca gttcccgacc ggggacatga gcaccgaaga ttatctggtc
    48721 aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt tattccctga cgaggaagaa
    48781 cgtgttaagc gccgcccgga atatgacgaa cgtctggaga ctgaacttca ggttatcaac
    48841 cagatgggct tcccgggcta cttcctcatc gttatggaat ttatccagtg gtcgaaagat
    48901 aacggcgtac cggtagggcc aggccgtggc tccggtgcgg gttcactggt ggcctacgcg
    48961 ctgaaaatta ccgacctcga tccgctggaa tttgacctgc tgttcgaacg tttccttaac
    49021 ccggaacgtg tctccatgcc tgacttcgac gttgacttct gtatggagaa acgcgatcag
    49081 gttatcgagc acgtagcgga catgtacggt cgtgatgcgg tatcgcagat catcaccttc
    49141 ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac
    49201 ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc cggggatgac gctggcgaaa
    49261 gcgtttgaag ccgagccgca gctgccggaa atctacgaag cggatgaaga agttaaggcg
    49321 ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta acgccggtaa gcacgccggt
    49381 ggggtggtta tcgcgccgac caaaattacc gattttgcgc cgctttactg cgatgaagag
    49441 ggcaaacatc cggtcaccca gtttgataaa agcgacgttg aatacgccgg actggtgaag
    49501 ttcgacttcc ttggtttgcg tacgctcacc atcatcaact gggcgctgga gatgatcaac
    49561 aagcggcggg cgaagaatgg cgagccgccg ctggatatcg ctgcgatccc gctggatgat
    49621 aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg
    49681 cgcggcatga aggacctgat caagcgtcta caacctgact gcttcgaaga tatgatcgcc
    49741 ctagtggcac tgttccgccc cggtccgttg caatcaggga tggtggataa ctttatcgac
    49801 cgtaaacatg gtcgtgaaga gatctcctat ccggacgtac agtggcagca tgaaagcctg
    49861 aaaccggtac tggagccaac ctacggcatt atcctgtatc aggaacaggt catgcagatt
    49921 gcgcaggtgc tttctggtta taccctcggt ggcgcggata tgctgcgtcg tgcgatgggt
    49981 aagaaaaagc cggaagagat ggctaagcaa cgttctgtat ttgctgaagg tgcagaaaag
    50041 aacggaatca acgctgaact ggcgatgaaa atcttcgacc tggtggagaa attcgctggt
    50101 tacggattta acaaatcgca ctctgcggcc tatgctttgg tgtcatatca aacgttatgg
    50161 ctgaaagcac actatcctgc ggagtttatg gcggcggtaa tgaccgccga tatggacaac
    50221 accgagaagg tggtgggtct ggtggatgag tgctggcgga tggggctgaa aatcctgcca
    50281 ccagatataa actccggtct ttaccatttc cacgtcaacg acgacggcga aatcgtgtat
    50341 ggtattggcg cgatcaaagg ggtcggtgaa ggtccgattg aggccatcat cgaagcccgt
    50401 aataaaggcg gctacttccg cgaactgttt gatctctgcg cccgtaccga caccaaaaag
    50461 ttgaaccgtc gcgtgctgga aaaactgatc atgtccgggg cgtttgaccg tcttgggcca
    50521 caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg
    50581 aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa
    50641 attgaacaat cctacgccag ctgccaaccg tggccggagc aggtggtatt agatggggaa
    50701 cgtgaaacgt taggcctgta cctgaccgga caccctatca accagtattt aaaagagatt
    50761 gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc
    50821 atcacggctg cggggctcgt tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt
    50881 atcggtatct gcacgctgga tgaccgttcc gggcggctgg aagtgatgtt gtttactgac
    50941 gccctggata aataccagca attgctggaa aaagaccgca tacttatcgt cagcggacag
    51001 gtcagctttg atgacttcag cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt
    51061 gacgaagccc gggaaaaata tgctcgcggg cttgctatct cgctgacgga caggcaaatt
    51121 gatgaccagc ttttaaaccg actccgtcag tctctggaac cccaccgctc tgggacaatt
    51181 ccagtacatc tctactatca gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg
    51241 tggcgtgtct ctccgagcga tcgtttatta aacgatctcc gtggcctcat tggttcggag
    51301 caggtggaac tggagtttga ctaatacagg aatactatga gtctgaattt ccttgatttt
    51361 gaacagccga ttgcagagct ggaagcgaaa atcgattctc tgactgcggt tagccgtcag
    51421 gatgagaaac tggatattaa catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta
    51481 gaactgacgc gtaaaatctt cgccgatctt ggtgcatggc agattgcgca actggcacgc
    51541 catccacagc gtccttatac cctggattac gttcgcctgg catttgatga atttgacgaa
    51601 ctggctggcg accgcgcgta tgcagacgat aaagctatcg tcggtggtat cgcccgtctt
    51661 gacggtcgtc cggtgatgat cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt
    51721 cgccgtaact ttggtatgcc agcgccagaa ggttaccgca aagcactgcg tctgatgcaa
    51781 atggctgaac gctttaagat gcctatcatc acctttatcg acaccccggg ggcttatccg
    51841 ggcgtgggcg cagaagaacg tggtcagtct gaagccattg cacgcaacct gcgtgaaatg
    51901 tctcgcctcg gtgtaccggt agtttgtacg gttatcggtg aaggtggttc tggcggcgcg
    51961 ctggcgattg gcgtgggcga taaagtgaat atgctgcaat acagcaccta ttccgttatc
    52021 tcgccggaag gttgtgcgtc cattctgtgg aagagcgctg acaaagcgcc gctggcagct
    52081 gaagcgatgg gtatcatcgc tccgcgtctg aaagaactaa aactgatcga ctccatcatc
    52141 ccggaaccgc tgggtggtgc tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg
    52201 caactgctgg cggatctggc cgatctcgac gtgttaagca ctgaagattt aaaaaatcgt
    52261 cgttatcagc gcctgatgag ctacggttac gcgtaattcg caaaagttct gaaaaagggt
    52321 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
    52381 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
    52441 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
    52501 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga
    52561 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
    52621 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
    52681 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
    52741 gtatgcgtca gtacaccgac gaatatcttg ataacattac accgccgttc acgaaagcct
    52801 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
    52861 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
    52921 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
    52981 cacacctgga agcagaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
    53041 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
    53101 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
    53161 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
    53221 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
    53281 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
    53341 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
    53401 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
    53461 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
    53521 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
    53581 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
    53641 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
    53701 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
    53761 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
    53821 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
    53881 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
    53941 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
    54001 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
    54061 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
    54121 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
    54181 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
    54241 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
    54301 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
    54361 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
    54421 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
    54481 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
    54541 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
    54601 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagtttt
    54661 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
    54721 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
    54781 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
    54841 tgatgacatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
    54901 ccgtgtcgat ccatacacgc aaaaacgctt caccttcttt aacgatccgg acgggctgcc
    54961 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
    55021 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
    55081 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
    55141 tggtgcagtg gcggacggaa aatccgggcg tcactctgcg cgctattcat gtgcatcacg
    55201 gtttaagtgc caatgccgat gcctgggtta cgcattgcga aaacgtctgc caacagtggc
    55261 aggtgccgct ggtggtcgaa cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
    55321 aggcgcggca ggcgcgttat caggcatttg cccgcacctt gttgcccggt gaagtgctgg
    55381 tcaccgcgca acatctcgac gatcaatgtg aaacttttct gctggcgcta aaacgcggta
    55441 gcggccctgc cgggctttcg gctatggcgg aagtttcgga gtttgccgga acacggctta
    55501 tccgcccgtt gctcgcccgc acgcggggag aactggagca gtgggcattg gcgcatggtt
    55561 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
    55621 tgcgcgtagt gccgttattg cagcagcgtt ggccgcattt tgcggaagca acggcccgca
    55681 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
    55741 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
    55801 cccgccgcgc ggcgattatc cgtcgctggc tggcagggca gaatgcacca atgccttccc
    55861 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
    55921 gtttacgctt gggcgcgttt gaaatccgcc gctatcagtc gcaactgtgg tggattaaat
    55981 ccgtcaccgg gcaaagtgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
    56041 taccggcggg gctgggaagc gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
    56101 cagacgaagc ggtcagcgtg cgtttcaaag cggcaggatt gctgcatatt gtcgggcgca
    56161 acggcggacg taagctgaag aaaatctggc aagagttagg cgtgccgccg tggctacgtg
    56221 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
    56281 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
    56341 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggac
    56401 tcgctcacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgt
    56461 agctcacggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
    56521 aaatcgctgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
    56581 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgta
    56641 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
    56701 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
    56761 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
    56821 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
    56881 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
    56941 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
    57001 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
    57061 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
    57121 cgtatgatgc tgcgcttgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
    57181 cgtggtttgt gtgccgatga tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
    57241 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
    57301 gccgcagaag tggcgctgtt tacctataat agtcgggcgg cgcaaatctg gtggcagcaa
    57361 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
    57421 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
    57481 ggcgtgatct ggttatcgga cgataagaat aatctggaag ttaacttaac cgcctggcaa
    57541 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
    57601 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggccattc
    57661 atctgcgttt tgacattcgg gcgtccagcc tgtcagagta ttacaaagag cgtctactcg
    57721 ccgccagcca tcatttgatc agcagtgatg gcgtgattgt cattaaggca caggaatacc
    57781 gcagtcagga actgaaccgc gaagccgcgc tagcccggct ggtggcagtg attaaagatt
    57841 taacaacaga acaaaaagcc cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca
    57901 ggctggcatc gaaagcacaa aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg
    57961 gtcgggaata aaaagaagga atagatggtg aaaaaagcga tagtgacagc gatggctgta
    58021 atcagcctct ttactctgat gggatgtaat aaccgggccg aagtcgatac gctttctcca
    58081 gcacaggctg ccgaactgaa accgatgccg caaagttggc gcggcgtgct gccgtgtgct
    58141 gattgcgaag gaatcgaaac ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat
    58201 gagcgttatc tcggtgctcg tgaagaacct tcctccttcg cttcctacgg tacatgggcg
    58261 cgaaccgctg acaagctggt attaaccgac agcaaaggtg aaaagtcata ttatcgggcg
    58321 aaaggcgatg cgctggagat gctcgatcgt gaaggcaatc cgattgaatc gcagttcaac
    58381 tatacgctgg aaccggcaca atctagttta cctatgacgc cgatgaccct gcgaggcatg
    58441 tatttttata tggctgatgc ggcgaccttc actgattgcg cgaccggaaa acgtttcatg
    58501 gtagcgaata acgcagagct ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa
    58561 ccggtgttac tgtcagtaga aggtcacttt acgcttgagg ctaatccgga tactggtgcg
    58621 ccaaccaaag tattagcgcc cgatacggcg ggtaaatttt acccgaataa ggattgcagt
    58681 agtttggggc agtaaacctg cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc
    58741 gcttatcagt atctacagca tataatgcaa cattttgaat tagcacgatt ttgtaggccg
    58801 gataaggcgt ttacgccgca tccggcattt tgtgcacgag gctggcttta tcactgagtc
    58861 agcccaacaa agcgacccgc cttgatataa atcccaggct tcaggtgccc aacatactgt
    58921 aactgcgttt ctggcttaaa cgcagagaca tcaccaatac gcatatgcat cagatcggca
    58981 gggctaatcc agcccgattg cgccagtgta agtggatgcc cggctttggc aaaggcatcc
    59041 gtgacaaatt ccgaacaaaa ccacgacttt ttgtctcctt cgcccacact gcttaactgc
    59101 gctttcgcca ggccgctgac gcactttggc gaaaatcctc ggagaacgga ttcagtgagc
    59161 acatctggcg agtcaccata aagggaataa attcgacaat gccgcgatag ttataaccgc
    59221 tatctttgat tttattggca aacgcggtga tatctgtggc ttgttgcggg gtaagatccg
    59281 ggactcgtaa gacgaaaagc ttatcactat gcttcatcgc ttttttaagg gaaacaatct
    59341 ggacgccagc gcctgtcgct tctgcaacgt tattatcacc cagaaagatt gcaacgtgac
    59401 tcacagagga agtgctgaag acgcggattc caaatgaggt taccccaagg cttgaggaga
    59461 acagcaaatc gccgggtttg agatcaggtg ccgttatttc ttttattgat tgttcggtga
    59521 aagagctttg atgctggaat ttgacagccc atgtttttgc ctcggcatct actgctgtgg
    59581 ctgatgggtc tggctggctg atatcaacgg tacaagccga aagtaaaaga aagctgggga
    59641 gaaacaggcg gcagtacgcc tttggtttat ccattttata caatccatgt aaaaaaaggg
    59701 ccctgaaatt caggaccctt tctggcatca gcctttaatc tgtttcacca gatattcgat
    59761 gatgtcacca gtcttaatta actgtttctc gccgttgcga cgatatttat attcgatatc
    59821 gtcgttgtcg aggttacggt cgcccagcac aatagtgtgc ggaataccga tcagttccat
    59881 atcagcaaac atcacgcccg gacgctcttt gcggtcatcc agcagcactt cgataccttg
    59941 tgcacgcagt tcgctgtaca gtttctcagc aagctcttgt acgcggaagg atttgtgcat
    60001 gttcatcggc agaatcgcca cctggaacgg cgcgatagcg tcaggccata cgatgcctcg
    60061 ttcgtcgtag ttctgctcaa tcgccgcagc taccacacgc gttaccccga taccgtagca
    60121 acccatcgtc aggatttggt tacggccatc ttcaccctgt acggaggctt tcagtgcttc
    60181 ggagtacttg gtacccagct ggaagatgtg accaacttcg ataccacgtt tgatcagcag
    60241 cgtaccctgg ccgtccgggc ttggatcgcc agccaccacg ttacggatat ctgcaacttc
    60301 cggggtagcg acatcgcgat cccagttgat gccgaagtag tgtttaccat cgatgttagc
    60361 accagcagcg aaatcactca tcgccgcaac ggtacggtca atcaccaccg gaatcggcat
    60421 gtttaccgga cccagtgaac ccggaccggc tttaaccacg gcacgaattt cttcttcggt
    60481 cgcgaaagtc agcgggcttg caacctgcgg cagtttttct gctttaactt cgttcagctc
    60541 gtgatcaccg cgcaccagca gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac
    60601 cagcagagtc ttaaccgttt tctcaatcgg cagattgaac tgttcaacca gttccgcgat
    60661 ggttttcgcg ttcggcgtat caaccagcgt catttcctgg gtagcagcag cgcgcggttc
    60721 tttcggcgcg atagcttctg ccagttcaat gttcgctgca tagtcagagg tgtcggagaa
    60781 gaccacatcg tcttcaccgc tctgcgccag cacctggaat tcgtgagagg cgctgccgcc
    60841 gatagaaccg gtgtcggctt gtacggcgcg gaaatccagc cccatgcggc tgaagatttt
    60901 gctgtaggcc gcatacattg catcgtaggt ttcctgcagg gattcctgag aagtatggaa
    60961 agagtaagca tctttcatca ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac
    61021 ttcgtcgcgg aacttggtct ggatctgata gaagttcagc ggcagctgtt tgtaagagct
    61081 aagctcgtta cgaatcaggt cagtgataac ttcttcatga gttgggccga gtacgaacgg
    61141 acgctcgcca cggtcaacaa aacgcagcag ttccggaccg tactgttccc aacgaccact
    61201 ctcttgccac aaatcagctg gctgaaccac cggcatcgac acctcgatcg caccggcgtt
    61261 gttcatctct tcacgcacga tgttttcgac ttttttcaga acgcgcacgc cggtcggcag
    61321 ccaggtatat aacccggagg ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg
    61381 gctgatcacc tcggcgtcgg caggtgtctc cttgagagtg gagagcaggt attggctagt
    61441 acgcatgttg ttacggttcc agttggaagg tagaacaggc tcaaggcgag cctgggacaa
    61501 aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg cgtgaattta
    61561 acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc gaacgttaaa
    61621 atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat aggccgggcg
    61681 tgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac gcttctccag
    61741 cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatctctg ccgctggcgc
    61801 actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga gatacggttt
    61861 gatatccact accggcgtac catcgaccag atccagactg ccgagcttca gaatcacgct
    61921 gtctttatgg caaacaacct ctttcagctc taccagcgac ataccaattg ggttagggcg
    61981 gaaagtagag cgtgttgcga aaacccccat tctggcgtta ccgccgaggc gcggtggacg
    62041 cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagga tccataaatg
    62101 gctgaacgct tccaggccgc gaacggcgtc ggcctggttg tagggagcaa tgagatgcag
    62161 ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt tttctttata
    62221 gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc cgtaatgtta
    62281 agcgcagagc cgatacatac agcctgacga tagcagcctg gcgtaccgct ggtgacttcg
    62341 cagctatgca gtaataccgc attggccttc attttcgagg cgttgatttg catccgctta
    62401 cgcgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga gtcgccactg
    62461 acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt ataaattcgg
    62521 accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg gggtgcagtg
    62581 ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag catgagtgct
    62641 actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa aacgtcaata
    62701 ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga atgatattac
    62761 aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt aaacactttg
    62821 tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt cacgttctcg
    62881 gcgtctttgt tatcttcacg cgttacgatc aggtttacgt acggggagtc tttatcttcg
    62941 acaaagatac cgtctttcgc tggagtcagg ccaatctggc tggcataggt ggtattgata
    63001 actgccagag cgatttgcgc gtcgtccaga gatcgaggca gctgcggtgc ttccagctca
    63061 acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag caggccaacg
    63121 ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg accaagatta
    63181 gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag tgatttgatt
    63241 ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac cagtttgtag
    63301 ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt ggcgtcgata
    63361 tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac cagctcaacg
    63421 tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg ctgttcggca
    63481 ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc gcagcctacc
    63541 agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt gaacgccata
    63601 ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt gacagcccgg
    63661 acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa taccagtacc
    63721 gtattcatca ccgtcgcgtt atagccgatg tagccatact gatagccaat ctgacctaaa
    63781 ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat cagggtgata
    63841 gttgccgcat tcaccagacc cggcaatgct tccggtaaca gcactttacg gacgatctgc
    63901 atcggcgtgg cacccattgc acgggaagct tcaattaacc cggttgggat ctccagcaga
    63961 gcgttctcga ccatacgggc aataaacggt gctgcaccaa cggttaacgg aacaatcgct
    64021 gcctgcaaac caatcgatgt accgacaata acgcgggtaa acggaatcat ccataccagc
    64081 aagataatga acgggatgga acggaaaatg ttcacaatcg cagaaacggt acgatacagc
    64141 ttcgcgttag caataatttg ccccgggcgc gtaacataaa gcaggacgcc gaccggcaga
    64201 ccaatcacaa agccaaaaaa tccggatacg aaggtcattg ccagcgtttc ccatacgcca
    64261 cgaaccagca accacatcat cggctcagac ataacccagt acctctactt ttacatggtg
    64321 ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca tttcagtcag
    64381 catgatgccg aacttaacgc caccggcgta atccatctgc gcgctaataa tgttgttgtt
    64441 gacgttgaaa cgacgcgcgg tttcagaaag cagcggggca tcgaccgatt gaccggtaaa
    64501 ctccaggcgc aacatcggca cgcagtcagt aaatggctcc gcttgcagac gttcctggta
    64561 atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca gcggcgtttt
    64621 cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat tgctgatgac
    64681 cgccacgcaa tcacaaatac gcttcacaac gtccatttcg tgggtgatca acagaatcgt
    64741 caaacccaga cggcggttaa tgtctttcag cagttcgaga atagaacgtg tcgttgccgg
    64801 gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg ctaatgcacg
    64861 ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc tatcatgctt
    64921 atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct cgtctttcgg
    64981 tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg aagagagcag
    65041 gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact cggattctga
    65101 cagcgtggtc agttcctggc catcgaccag cacgctaccc tcggttgggc gctccagcag
    65161 gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa cgccataaat
    65221 ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc gggtgccctg
    65281 gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat cgtcattaag
    65341 ttagtcgtgg catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat ggatgttaag
    65401 gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc aggtcgaaac
    65461 atgcgatact agcgtcccat gccttattaa ggagctataa aaggtggcga agagcgtacc
    65521 cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg tccatgagat
    65581 cgacaacttt gaatttatcg acggtgttat tgacaccatg cgcgagctaa aaaaaatggg
    65641 ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat ttaccgaagc
    65701 acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag atgtcgatct
    65761 ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt ttcgccaggt
    65821 ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt atttgcatat
    65881 tgatatggcc gcttcttata tggtgggaga taaattagaa gatatgcagg cagcggttgc
    65941 ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac ctgaagcaga
    66001 aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga taaaaaagca
    66061 gcaaaaaccg gcgcaatgat taaaagatga gcggttgaaa taaaaatgca tttttccgct
    66121 tgtcttcctg agccgactcc ctataatgcg cctccatcga cacggcggat gtgaatcact
    66181 tcacacaaac agccggttcg gttgaagag
//
LOCUS       DAZGTR010000011        66066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGTR010000011 DAZGTR010000000
VERSION     DAZGTR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.011"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            118..387
                     /locus_tag="ACHLCY_000821"
     CDS             118..387
                     /locus_tag="ACHLCY_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0368136.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            356..1474
                     /locus_tag="ACHLCY_000822"
     CDS             356..1474
                     /locus_tag="ACHLCY_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0368137.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWKKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            1641..2435
                     /gene="potC"
                     /locus_tag="ACHLCY_000823"
     CDS             1641..2435
                     /gene="potC"
                     /locus_tag="ACHLCY_000823"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP0368138.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            2432..3478
                     /gene="potD"
                     /locus_tag="ACHLCY_000824"
     CDS             2432..3478
                     /gene="potD"
                     /locus_tag="ACHLCY_000824"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP0368139.1"
                     /translation="MKKWSRHLLAAGTLALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPAYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            3536..3997
                     /locus_tag="ACHLCY_000825"
     CDS             3536..3997
                     /locus_tag="ACHLCY_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP0368140.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSCP"
     gene            3994..4782
                     /gene="ycfZ"
                     /locus_tag="ACHLCY_000826"
     CDS             3994..4782
                     /gene="ycfZ"
                     /locus_tag="ACHLCY_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0368141.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(4921..5742)
                     /gene="cobB"
                     /locus_tag="ACHLCY_000827"
     CDS             complement(4921..5742)
                     /gene="cobB"
                     /locus_tag="ACHLCY_000827"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP0368142.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(5758..6669)
                     /gene="nagK"
                     /locus_tag="ACHLCY_000828"
     CDS             complement(5758..6669)
                     /gene="nagK"
                     /locus_tag="ACHLCY_000828"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP0368143.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(6698..7942)
                     /gene="lolE"
                     /locus_tag="ACHLCY_000829"
     CDS             complement(6698..7942)
                     /gene="lolE"
                     /locus_tag="ACHLCY_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP0368144.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(7942..8643)
                     /gene="lolD"
                     /locus_tag="ACHLCY_000830"
     CDS             complement(7942..8643)
                     /gene="lolD"
                     /locus_tag="ACHLCY_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP0368145.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(8636..9835)
                     /gene="lolC"
                     /locus_tag="ACHLCY_000831"
     CDS             complement(8636..9835)
                     /gene="lolC"
                     /locus_tag="ACHLCY_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP0368146.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            10097..11170
                     /gene="ycfT"
                     /locus_tag="ACHLCY_000832"
     CDS             10097..11170
                     /gene="ycfT"
                     /locus_tag="ACHLCY_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP0368147.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            11298..14744
                     /gene="mfd"
                     /locus_tag="ACHLCY_000833"
     CDS             11298..14744
                     /gene="mfd"
                     /locus_tag="ACHLCY_000833"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP0368148.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRHTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            14888..15850
                     /gene="ldtC"
                     /locus_tag="ACHLCY_000834"
     CDS             14888..15850
                     /gene="ldtC"
                     /locus_tag="ACHLCY_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP0368149.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTRPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(15933..16190)
                     /gene="bhsA"
                     /locus_tag="ACHLCY_000835"
                     /gene_synonym="ycfR"
     CDS             complement(15933..16190)
                     /gene="bhsA"
                     /locus_tag="ACHLCY_000835"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP0368150.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            16431..17063
                     /gene="comR"
                     /locus_tag="ACHLCY_000836"
     CDS             16431..17063
                     /gene="comR"
                     /locus_tag="ACHLCY_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP0368151.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(17125..17664)
                     /gene="ycfJ"
                     /locus_tag="ACHLCY_000837"
     CDS             complement(17125..17664)
                     /gene="ycfJ"
                     /locus_tag="ACHLCY_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0368152.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(17891..19195)
                     /gene="ndh"
                     /locus_tag="ACHLCY_000838"
     CDS             complement(17891..19195)
                     /gene="ndh"
                     /locus_tag="ACHLCY_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP0368153.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(19595..20137)
                     /gene="ycfP"
                     /locus_tag="ACHLCY_000839"
     CDS             complement(19595..20137)
                     /gene="ycfP"
                     /locus_tag="ACHLCY_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP0368154.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERLLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(20160..21185)
                     /gene="nagZ"
                     /locus_tag="ACHLCY_000840"
     CDS             complement(20160..21185)
                     /gene="nagZ"
                     /locus_tag="ACHLCY_000840"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP0368155.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(21196..22020)
                     /gene="thiK"
                     /locus_tag="ACHLCY_000841"
     CDS             complement(21196..22020)
                     /gene="thiK"
                     /locus_tag="ACHLCY_000841"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP0368156.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(22001..22642)
                     /gene="lpoB"
                     /locus_tag="ACHLCY_000842"
     CDS             complement(22001..22642)
                     /gene="lpoB"
                     /locus_tag="ACHLCY_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP0368157.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(22656..23033)
                     /gene="ycfL"
                     /locus_tag="ACHLCY_000843"
     CDS             complement(22656..23033)
                     /gene="ycfL"
                     /locus_tag="ACHLCY_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP0368158.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMGSSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(23036..23395)
                     /gene="hinT"
                     /locus_tag="ACHLCY_000844"
     CDS             complement(23036..23395)
                     /gene="hinT"
                     /locus_tag="ACHLCY_000844"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP0368159.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            23729..25918
                     /gene="fhuE"
                     /locus_tag="ACHLCY_000845"
     CDS             23729..25918
                     /gene="fhuE"
                     /locus_tag="ACHLCY_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP0368160.1"
                     /translation="MLSTQFNRDNQYQASTKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQHIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLIGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(25978..27411)
                     /gene="ptsG"
                     /locus_tag="ACHLCY_000846"
     CDS             complement(25978..27411)
                     /gene="ptsG"
                     /locus_tag="ACHLCY_000846"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP0368161.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(27706..28503)
                     /gene="ycfH"
                     /locus_tag="ACHLCY_000847"
     CDS             complement(27706..28503)
                     /gene="ycfH"
                     /locus_tag="ACHLCY_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0368162.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(28514..29518)
                     /gene="holB"
                     /locus_tag="ACHLCY_000848"
     CDS             complement(28514..29518)
                     /gene="holB"
                     /locus_tag="ACHLCY_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP0368163.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(29515..30156)
                     /gene="tmk"
                     /locus_tag="ACHLCY_000849"
     CDS             complement(29515..30156)
                     /gene="tmk"
                     /locus_tag="ACHLCY_000849"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0368164.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(30146..31168)
                     /gene="yceG"
                     /locus_tag="ACHLCY_000850"
     CDS             complement(30146..31168)
                     /gene="yceG"
                     /locus_tag="ACHLCY_000850"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP0368165.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(31171..31980)
                     /gene="pabC"
                     /locus_tag="ACHLCY_000851"
     CDS             complement(31171..31980)
                     /gene="pabC"
                     /locus_tag="ACHLCY_000851"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP0368166.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMSVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(32100..33341)
                     /gene="fabF"
                     /locus_tag="ACHLCY_000852"
     CDS             complement(32100..33341)
                     /gene="fabF"
                     /locus_tag="ACHLCY_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP0368167.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(33429..33665)
                     /gene="acpP"
                     /locus_tag="ACHLCY_000853"
     CDS             complement(33429..33665)
                     /gene="acpP"
                     /locus_tag="ACHLCY_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0368168.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(33876..34610)
                     /gene="fabG"
                     /locus_tag="ACHLCY_000854"
     CDS             complement(33876..34610)
                     /gene="fabG"
                     /locus_tag="ACHLCY_000854"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP0368169.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNEIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(34623..35552)
                     /gene="fabD"
                     /locus_tag="ACHLCY_000855"
     CDS             complement(34623..35552)
                     /gene="fabD"
                     /locus_tag="ACHLCY_000855"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP0368170.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(35568..36521)
                     /gene="fabH"
                     /locus_tag="ACHLCY_000856"
     CDS             complement(35568..36521)
                     /gene="fabH"
                     /locus_tag="ACHLCY_000856"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP0368171.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(36589..37659)
                     /gene="plsX"
                     /locus_tag="ACHLCY_000857"
     CDS             complement(36589..37659)
                     /gene="plsX"
                     /locus_tag="ACHLCY_000857"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP0368172.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(37740..37913)
                     /gene="rpmF"
                     /locus_tag="ACHLCY_000858"
     CDS             complement(37740..37913)
                     /gene="rpmF"
                     /locus_tag="ACHLCY_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP0368173.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(37965..38486)
                     /gene="yceD"
                     /locus_tag="ACHLCY_000859"
     CDS             complement(37965..38486)
                     /gene="yceD"
                     /locus_tag="ACHLCY_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP0368174.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            38685..39269
                     /gene="yceF"
                     /locus_tag="ACHLCY_000860"
     CDS             38685..39269
                     /gene="yceF"
                     /locus_tag="ACHLCY_000860"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP0368175.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(39381..40340)
                     /gene="rluC"
                     /locus_tag="ACHLCY_000861"
     CDS             complement(39381..40340)
                     /gene="rluC"
                     /locus_tag="ACHLCY_000861"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP0368176.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            40913..44098
                     /gene="rne"
                     /locus_tag="ACHLCY_000862"
     CDS             40913..44098
                     /gene="rne"
                     /locus_tag="ACHLCY_000862"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP0368177.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(44294..45247)
                     /gene="flgL"
                     /locus_tag="ACHLCY_000863"
     CDS             complement(44294..45247)
                     /gene="flgL"
                     /locus_tag="ACHLCY_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP0368178.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(45259..46902)
                     /gene="flgK"
                     /locus_tag="ACHLCY_000864"
     CDS             complement(45259..46902)
                     /gene="flgK"
                     /locus_tag="ACHLCY_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP0368179.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGALGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(46968..47909)
                     /gene="flgJ"
                     /locus_tag="ACHLCY_000865"
     CDS             complement(46968..47909)
                     /gene="flgJ"
                     /locus_tag="ACHLCY_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP0368180.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKEGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(47909..49006)
                     /gene="flgI"
                     /locus_tag="ACHLCY_000866"
     CDS             complement(47909..49006)
                     /gene="flgI"
                     /locus_tag="ACHLCY_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP0368181.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(49018..49716)
                     /gene="flgH"
                     /locus_tag="ACHLCY_000867"
     CDS             complement(49018..49716)
                     /gene="flgH"
                     /locus_tag="ACHLCY_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP0368182.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(49769..50551)
                     /gene="flgG"
                     /locus_tag="ACHLCY_000868"
     CDS             complement(49769..50551)
                     /gene="flgG"
                     /locus_tag="ACHLCY_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP0368183.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(50689..51444)
                     /gene="flgF"
                     /locus_tag="ACHLCY_000869"
     CDS             complement(50689..51444)
                     /gene="flgF"
                     /locus_tag="ACHLCY_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP0368184.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(51464..52669)
                     /gene="flgE"
                     /locus_tag="ACHLCY_000870"
     CDS             complement(51464..52669)
                     /gene="flgE"
                     /locus_tag="ACHLCY_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP0368185.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(52694..53389)
                     /gene="flgD"
                     /locus_tag="ACHLCY_000871"
     CDS             complement(52694..53389)
                     /gene="flgD"
                     /locus_tag="ACHLCY_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP0368186.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPKGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(53401..53805)
                     /gene="flgC"
                     /locus_tag="ACHLCY_000872"
     CDS             complement(53401..53805)
                     /gene="flgC"
                     /locus_tag="ACHLCY_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP0368187.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(53809..54225)
                     /gene="flgB"
                     /locus_tag="ACHLCY_000873"
     CDS             complement(53809..54225)
                     /gene="flgB"
                     /locus_tag="ACHLCY_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP0368188.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            54380..55039
                     /gene="flgA"
                     /locus_tag="ACHLCY_000874"
     CDS             54380..55039
                     /gene="flgA"
                     /locus_tag="ACHLCY_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP0368189.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            55115..55408
                     /gene="flgM"
                     /locus_tag="ACHLCY_000875"
     CDS             55115..55408
                     /gene="flgM"
                     /locus_tag="ACHLCY_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP0368190.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            55413..55829
                     /gene="flgN"
                     /locus_tag="ACHLCY_000876"
     CDS             55413..55829
                     /gene="flgN"
                     /locus_tag="ACHLCY_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP0368191.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(55869..57404)
                     /gene="murJ"
                     /locus_tag="ACHLCY_000877"
     CDS             complement(55869..57404)
                     /gene="murJ"
                     /locus_tag="ACHLCY_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP0368192.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(57514..58437)
                     /gene="yceM"
                     /locus_tag="ACHLCY_000878"
     CDS             complement(57514..58437)
                     /gene="yceM"
                     /locus_tag="ACHLCY_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0368193.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(58439..59086)
                     /gene="yceH"
                     /locus_tag="ACHLCY_000879"
     CDS             complement(58439..59086)
                     /gene="yceH"
                     /locus_tag="ACHLCY_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP0368194.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(59097..59681)
                     /gene="rimJ"
                     /locus_tag="ACHLCY_000880"
     CDS             complement(59097..59681)
                     /gene="rimJ"
                     /locus_tag="ACHLCY_000880"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0368195.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            59917..61125
                     /gene="mdtH"
                     /locus_tag="ACHLCY_000881"
     CDS             59917..61125
                     /gene="mdtH"
                     /locus_tag="ACHLCY_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP0368196.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            61189..61836
                     /gene="grxB"
                     /locus_tag="ACHLCY_000882"
     CDS             61189..61836
                     /gene="grxB"
                     /locus_tag="ACHLCY_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP0368197.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            61970..62530
                     /gene="yceB"
                     /locus_tag="ACHLCY_000883"
     CDS             61970..62530
                     /gene="yceB"
                     /locus_tag="ACHLCY_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0368198.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDTNLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYILREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            62636..63682
                     /gene="pyrC"
                     /locus_tag="ACHLCY_000884"
     CDS             62636..63682
                     /gene="pyrC"
                     /locus_tag="ACHLCY_000884"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP0368199.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            63755..64000
                     /gene="dinI"
                     /locus_tag="ACHLCY_000885"
     CDS             63755..64000
                     /gene="dinI"
                     /locus_tag="ACHLCY_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP0368200.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            64290..64544
                     /gene="bssS"
                     /locus_tag="ACHLCY_000886"
     CDS             64290..64544
                     /gene="bssS"
                     /locus_tag="ACHLCY_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP0368201.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            64659..65777
                     /gene="solA"
                     /locus_tag="ACHLCY_000887"
     CDS             64659..65777
                     /gene="solA"
                     /locus_tag="ACHLCY_000887"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP0368202.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTVIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            65825..65950
                     /locus_tag="ACHLCY_000888"
     CDS             65825..65950
                     /locus_tag="ACHLCY_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP0368203.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYFSFPH"
BASE COUNT        16222 a        17575 c        16410 g        15859 t
ORIGIN      
        1 caaaatacgt ttcagttatc tggcaaaaac tgatcccgag tcaggctgat ttttaatgtt
       61 caaccgtcgc cagttgtcgt tgatattctg caactggcgc gttccggagt gatagccatg
      121 agcgaacagt acctgataac gctcgacgag tggaaaccaa aacggttcag tctcccaata
      181 acaaacacta ccctggtgaa atacggaaaa ctaggataca tcgttccaag accacaaaaa
      241 attcgtgggc gttggctgat agatcgccga gcagtatttg ttgggcctgg tgaaacggga
      301 attgcgccgg aaattcatac tggcgatgat gatgcactga aggagatttt aactcatgtc
      361 accgaggcca cgaaaaaaca gcactgacgt agccggtctt tacgaaaagt ttgatcgcag
      421 aactggcaga gtttactacc agtataaaaa tcctgtgact ggaaaatttc acggactcgg
      481 aacagacaaa ggtaaggcag aaaaaatcgc ttccacagcc aatcagcgga tagctgcagc
      541 agaagctgaa tatttcatgc gcaaaattga tgaaagtccg tcagcaacaa aacgtcgggg
      601 tatcagatta aaggcatggg ttgatcgata tctgaaaata caggacacgc gactgaaaaa
      661 tggagatatt gcagctacaa ctcacaaaga aaaaactcga atggctgcat acctggtttc
      721 ccgtctggga aaccacccat tgaaagaact ggaagtaaga gactttgcat taatactgga
      781 tgagtggctg gataaagaca tggtcagcac agcgagagta aatcgtggat tatgggttga
      841 tatttataaa gaagcacagc atgcagggga agttcctcct ggatggaatc ctccggaggc
      901 tacccgtaaa ccgatcccta aagtaaccag agccaggctc accatggaag actggaaaaa
      961 aatttacaat gcaacgcctg aaaaacactt tatccgtaac gcaatgcttc ttgcgattgt
     1021 tactggtcag cgccgtgatg acatttgcca catgcgtttt tcagatgtgt ggaacgaaca
     1081 cttgcatatc acccagggaa aaaccggaat gcgtctggcg ttaccgctta cactacgctg
     1141 tgatgccatt gggataacgt taaaagaagt tattgatggg tgccgagaca gaatattaag
     1201 tccatatcta atccatagtc ggcaccagaa acaaccgaag ccgatgagta aagacaacct
     1261 gagcgactac tttgccaaag cacgggatct ggctgggata attccaccag caggaaaaac
     1321 tccgccaaca tttcatgaac aacgttctct atcagaacgg ctgtatcgtg cacagggtat
     1381 cgatacaaaa acattactag gacataaagt ccaggcaacc accgaccgct ataacgatac
     1441 tcgaggtcag gaatgggtta agttggttat ttgacaaaca ccatatggaa tatccgtcac
     1501 catctcagga aaaaagtgtt gtaaaatgcg tggccttgtt ttggggaaaa gttttggaga
     1561 gattttggag aaaggaaaaa attgaataaa ttcaaaatcc tgaaagtgct ccaccttatg
     1621 aataaaaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
     1681 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
     1741 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
     1801 acagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacatttg
     1861 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgtggtaagc
     1921 cgttcgttag cggaatgctg tttgtggtga tgatgtcacc agatatcgtg atggcgattt
     1981 ctctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
     2041 cgcatatcac cttctgcctg ccgtttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
     2101 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagtgaattt accattctgc
     2161 gaaaaatcat tctgccactg gcaatgcctg cggtggcggc gggctgggtg ttaagcttta
     2221 ccctgtcgat ggacgatgtg gtagtttctt cgttcgtcac cggaccaagt tatgaaattc
     2281 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
     2341 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
     2401 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
     2461 ggcgggtact ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
     2521 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
     2581 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
     2641 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
     2701 gcgtaaagaa gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
     2761 cgatccagat atgctcaaca agccgtttga cccgaataac gattactcca ttccgtatat
     2821 ctggggtgcg acggcgattg gtgttaacgg tgatgcggtg gatccgaaat ctgtcaccag
     2881 ctgggccgat ctgtggaagc ctgcgtacaa aggcagcctg ctgttgaccg acgatgcccg
     2941 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
     3001 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
     3061 ctccgataac ccagctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
     3121 cggttctgct ttcgttgcac ggcaggcggg tacgccaatt gacgtggtgt ggccgaaaga
     3181 aggcggcatt ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
     3241 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
     3301 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagcccgg aagtggcgaa
     3361 cgataaaacg ctctacccgg atgctgaaac cattaaaaat ggtgaatggc agaatgacgt
     3421 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
     3481 cagcaagcgg gcggtggcat taccgcccgt tttttattta gcaaggatgt ggctaatgag
     3541 tcaggacagt aaagtgttat cccgcgcttt tctcggtatc ggtctgatat tgctcgttat
     3601 ttctgcaatc atcttttatt accattttac ttttaccaaa agcgcggttc atacagaagg
     3661 aatcattgtt gatgccgttt ggtacaacaa tcactcaaac gacgtcgatg ataatggttc
     3721 ctggtatcca gtggttgcct ttcgccctac gccagattac acgctaattt tcaattcgaa
     3781 tatcggtagt gatttttatg aagatagcga aggtgacaga gtcaatgtct attatccgcc
     3841 agggcatcca gaacaagccg aaataaacaa tccctgggtt aattttttta aatggggctt
     3901 tgttggcata gcaggcatta ttttcggtag tgtcggtctg ataatcacgc tcccctccac
     3961 caaaaaatct cgcaggaaac ggaagtcctg cccatgaaaa aattcattat actgctcagt
     4021 ttactgatac tcctgcctct ggtagcaacc agtaaaccat tgattccaat aatgaagaca
     4081 ctttttacgg atgttaccgg tactgtaccg gatgctgaag agatcgcacg taaagccgag
     4141 ctttttcgcc aacaaacggg aattgcaccg tttattgtga ttttacccga cattaataat
     4201 gaagccagtc tcagacaaaa tggtaaagcg atgctggcgc atgcgtcatc ttcattgagt
     4261 aatgtaaaag gaagtgttct gttactattt actacccgcg aaccacggtt aattatgatc
     4321 accaacggcc aggttgaaag tggtctggac gataaacatc tcggccttct gatagaaaat
     4381 cacacgctgg cttatttaaa tgcagatctc tggtatcagg gaatcaataa tgcattggct
     4441 gttctacaag cacagatatt aaaacaaccg acgccgccac tgacgtatta tccgcatcca
     4501 ggacaacagc atgaaaatgc tccccccggc agcaccaaca cattaggttt tatcgcctgg
     4561 gctgcgacgt ttatcttatt ttcctggatc ttttgctaca cgactcgttt tatttatgcg
     4621 ttaaaatttg cagtggcgat gactattgcc aatatgggtt atcaggcttt atgtttgtat
     4681 atcgataata gctttgcgat taccagaata tcgccactct gggcagggct aattggtgtc
     4741 tgtacgttta ttgccgcatt gttattgacg agtaaacgat aaaagaggcc ggaaaagtgc
     4801 gcgtgtatta tgccggatgg ggcgcgaacg ccttatccgg cccacaaaac ccgcaaattc
     4861 aattaattgc gtccccttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct
     4921 ttataatccc ttcagcaact tttcaacaaa ctcaggcacc acctggcttg ccgggccgta
     4981 atatttctcg gcaaattcat taccgacctg acttggttcg agattcagtt ccacggtgtg
     5041 cgcgccatgc agtttcgctt cgtgaacaaa cccagccgcc ggataaacat gcccggaagt
     5101 accaatggca atgaaaatat cagccatcga caacgccata taaatttcat ccatgccgag
     5161 tggcatttcg ccaaaccaaa cgacgtgcgg gcgcaagggt gccgggaact ggcaacaatg
     5221 gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca
     5281 acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg
     5341 caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagggcgt cttgcagttt
     5401 agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg
     5461 ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac
     5521 atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga
     5581 ttccgcagaa atccctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg
     5641 caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc ggcgtttatt
     5701 tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta
     5761 atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg
     5821 ttcaatgcgc ggaacacgag ctacaggtaa gagatgacga ggcagcctgt ccgccagttg
     5881 cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac
     5941 aatggtcagg atatttccca gacaaaccgc taataaatcc agataacgct caacgtgcgc
     6001 ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct gcaacggttg
     6061 atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca
     6121 gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca tggttaacgc
     6181 atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac cggtaatcgg
     6241 tttgccgttg aaaatcagcc cgccgccaac gccggtgccg agaatcaacc ccatcaccag
     6301 tggatattga gtaaattcat catcccaggc ttctgaaagg gcaaaacagt tggcatcgtt
     6361 atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttaccgct
     6421 ggcagcgggg acattggcgg catacagcgt accatcttct gtttccggca ttcccggaat
     6481 accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta ccagctcaca
     6541 cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg
     6601 caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc
     6661 gtaatacatt gccgctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg
     6721 cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca
     6781 atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaaatcaa
     6841 taaaatagat atcgctggag aggaactgat gaccgatcaa cttttcaatc cactcaataa
     6901 tcggggtaag ttgcagtgaa accaccacac cgataatcac cccacacagg ctgccgaaca
     6961 gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca
     7021 gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga
     7081 cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga
     7141 tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta atataaacat
     7201 agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca
     7261 ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca
     7321 gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaacgt
     7381 gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg gggatcataa
     7441 tcgacaccca atccccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa
     7501 tttgctgttc gcccgcttta aaattgcgcc acgcatcgcc ctggacaaac gagggtaatg
     7561 cgctcagacg ctgttcctgt tgcgggttaa cgcccttcac ctggattgcg cgaagattcg
     7621 cgccactttc caccagcccg gtgaaattga tatacggcgc ggcagcggca atacccggca
     7681 ctttttgcac gtgatccagt gcttcctgcc agttagtcca cggctgatcc accgcctcga
     7741 tttcaccatg cggcaccacc gccagaatac ggttgttcag ttcgcgttca aagccgttca
     7801 tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg gtagaaataa
     7861 cggagatcag cgacaccatg ccgccgcgcc gccgtccgcg actaaaacgc aggccaatta
     7921 ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg
     7981 accatcacgc atttctaact ggcggctcat acgtttcgcc agttgcaggt cgtgagtaac
     8041 caccaggaag gcggtgccct gcaagcgatt caattcccca agcaactgga agatgctgtc
     8101 ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccaggc gcgggttatt
     8161 caccagcgca cgggcaatcg ccacacgctg gcgttcgccg ccagaaagtt cagatgggcg
     8221 gtgattcgca cgatgatcca gccccaccgc ttttaacatc tcaagtgcac ggctgttgat
     8281 ttcagcgggc tttttcttgc caatcagcag cggcatagcc acgttttcca gggcggtaaa
     8341 atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagctctgc
     8401 tttcgccgcc gaagacagtt tgctcatcgg ttgaccgtta aagatcacat cgccggaggt
     8461 tggcgtatcc agcccgccca acaagtgcag caaggtgctt ttaccggaac cagagctacc
     8521 gacgatcgcc atcatttcac cttcgccgac gctgaaactg acattgtgca gcacatcggt
     8581 ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt
     8641 cataacgtaa agcctcagcg ggttgagtgg cggcagcgcg ccatgaaggg taaagcgtag
     8701 acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag
     8761 cgaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact
     8821 ggctggcaag cagtgcgcca agcgccgcac cgaggatcgc accgataatc ccggcgctgg
     8881 ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga
     8941 tcgcgacttc gccctgcttc tccattacca tcagccctag tgaggtaata atgttaaacg
     9001 ccgcaacggc gacaatcagg ctcagcagca agcccatcat atttttttcc atgcgtacgg
     9061 cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt
     9121 tttgctgact taaagagtca actttcagcg gctcatccag ccacaaacgc cagccggtaa
     9181 tattgcctgc cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat
     9241 agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg cgctggcttg
     9301 gaatacgccc catcggcgtg aactggctgg cagatggtac catcacgcgg atttgatcac
     9361 cgcgattaac gcctagctgt gaggcaagtt gttcgccgag gataacatta tatttccccg
     9421 gctcgaggtc agtttgtttc acattgacca gatacggtgt aagtggatct ttttgcgccg
     9481 ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac
     9541 cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt
     9601 gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga
     9661 tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca
     9721 tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg
     9781 ctgcacgccc acgcatgtaa cgcaggccaa taaatagagc gacaggttgg tacatgaaat
     9841 ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc
     9901 tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat
     9961 tgccatgttt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag
    10021 acagataccc attacacctc gggtcataac ccgcaggatc aaccgcatgt aaattaaaaa
    10081 agagcgtgtt gtcctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg
    10141 tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcatc tgaccacttt
    10201 tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc
    10261 cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga
    10321 cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc
    10381 gctttggggc gtggtgcagt ggctggcgtt aagtgcgctg aaccagtggc tggcacctga
    10441 gcgcgattta agcaatgcct ccaatgccgc ttatgccgat tccaccggtg agttcctgca
    10501 cgggatgatc accgccagca ccagcttgtg gtatctgtat gcgttaattg tctatttcgt
    10561 ggtatgtaaa atttttagcc gtctggcgct gccactattc gccttgtttg tactgctgag
    10621 tgtggcggtt aatttcgttc ccacgccgtg gtggggaatg aacagtgtga tccgcaattt
    10681 gctttattac agccttggcg catggtttgg cgcaacaata atgacctgtg ttaaagaggt
    10741 gccgttgcgc cgccatctgc tgatggcttc tttgcttacc gttctggcgg tcggtgcctg
    10801 gttgtttact atctcgctgc tgttgtcgct ggtatcgatt gtggtgatca tgaagctgtt
    10861 ttatcaatac gagcaacgat tcggtatgcg ctccaccagc ctgctgaatg tgattggctc
    10921 caacaccatt gctatctaca ccacccatcg cattctggtt gaaatattca gcttaactct
    10981 gcttgcgcaa atgaacgcag cacgctggtc gccgcaagtc gaactgacac tcctgctggt
    11041 ttatcccttt gtcagtttgt tcatctgtac tgttgcgggc ttgctggtaa gaaaactttc
    11101 acagcgtgca ttcagcgatc tgttgttctc cccgccttca ctgcccgctg ccgtcagtta
    11161 ctctcgttaa accatcgccc cagccggggc gattcaattt gctaaaccat gtcgattacg
    11221 gataatgaac agcattgctt atcagagata tgcccccata tgttgaggca tatcctaacg
    11281 agaatctgac aaccgttatg cctgaacaat atcgttatac gctgcccgtc aaagcgggtg
    11341 agcagcgtct gctgggcgag ttaaccggcg cagcctgtgc aacgctggta gcggaaattg
    11401 ccgaacgtca cgccggtccg gtggtactca ttgcaccaga tatgcaaaat gctctgcgtt
    11461 tgcatgatga aatcagccag ttcaccgatc agatggtgat gaatctggcg gactgggaaa
    11521 ctcttcccta cgacagtttt tcgcctcatc aggacattat ctcctcgcgc ctttccaccc
    11581 tttaccagct accgacgatg cagcgtggcg tactgattgt tccggtgaat acgcttatgc
    11641 agcgcgtttg cccacacagt tttctccacg gtcatgcgct ggtgatgaaa aaaggtcagc
    11701 gcctgtcacg agatgcatta cgaacccaac tggacagcgc cggttatcgc catgttgacc
    11761 aggtgatgga gcacggcgaa tacgccacgc gcggcgcgtt gctggatctc ttcccgatgg
    11821 ggagtgagct gccttatcgt cttgatttct ttgatgatga aatcgacagc ctgcgggtgt
    11881 ttgacgtcga cagccagcgc acgctggagg aagtagaagc gatcaatctg ctgcccgcgc
    11941 acgaatttcc gaccgataaa gcggcaattg aactgttccg cagccagtgg cgcgatacct
    12001 tcgaagtgaa gcgcgatccg gaacatattt accagcaagt gagtaaaggc acattacctg
    12061 ccgggatcga gtactggcag ccattgttct tcagcgaacc actgccgccg ctgttcagtt
    12121 atttccctgc caataccttg ctcgtgaata ctggcgatct ggaaaccagt gccgaacgtt
    12181 tccaggctga cacgctggcg cgttttgaga atcgcggcgt cgatccgatg cgcccgctgt
    12241 tgccaccaca atcgctctgg ctgcgggtgg acgagctctt ctcagagctg aaaaactggc
    12301 cccgggtgca gctaaaaact gaacatttac cgacaaaagc cgcgaatgcc aatttaggtt
    12361 tccagaaact gccagacctg gccgttcagg cacaacaaaa agcgccgctg gatgcgctgc
    12421 gtaagttcct cgagactttc gacggtccgg tggtgttctc ggtagaaagt gaaggtcgcc
    12481 gtgaagcgct gggtgaactg ctcgcacgaa ttaaaattgc tccgcaacgc attatgcgtc
    12541 ttgatgaagc cagcgaccgt gggcgttatc tgatgattgg cgctgccgaa catggttttg
    12601 tcgataaggt gcgtaatctg gcgctgatct gcgaaagcga tctgctcggt gaacgtgttg
    12661 cccgccgtcg tcaggattct cgccacacca tcaaccccga tacactgatc cgtaaccttg
    12721 cggagctgca tattggtcag ccggtggtcc atctggagca cggtgtcggg cgctatgccg
    12781 gaatgaccac gctcgaagcg ggcggcatta ctggcgagta tttgatgctc acctatgcca
    12841 acgacgccaa actgtatgtt ccggtgtcgt cactgcatct gattagccgt tacgcaggtg
    12901 gcgcggaaga aaacgccccg ctgcataaac ttggcggcga tgcgtggtca cgcgcgcggc
    12961 agaaagcggc ggaaaaagtg cgtgatgtgg cggcggaatt gctggatatc tacgcgcaac
    13021 gcgccgccaa agagggcttc gcgtttaaac acgatcgtga gcagtatcag ttgttctgcg
    13081 acagcttccc gtttgaaacc acgccggatc aggcgcaggc cattaatgcg gtacttagcg
    13141 acatgtgtca gccgctggca atggatcgtc tggtgtgcgg cgatgttggc tttggtaaaa
    13201 cagaagtggc gatgcgcgcc gctttcctgg cagtagataa ccacaagcag gtggcggtgt
    13261 tggtgcctac cacccttctc gcgcagcagc attacgacaa cttccgcgac cgtttcgcca
    13321 actggccggt acgtatcgaa atgatctccc gtttccgcag cgccaaagag cagacgcaaa
    13381 tccttgcgga agtggcggaa gggaaaatcg atattctgat cggtacgcac aaactgctgc
    13441 aaagtgacgt caagtttaaa gatttaggcc tgctgattgt cgatgaagaa caccgcttcg
    13501 gggtgcgtca taaagagcgt attaaagcga tgcgcgcgaa cgtggatatt ctgacgctta
    13561 ctgcaacgcc gatcccacgt acgctgaata tggcaatgag cggaatgcgt gacctgtcga
    13621 ttatcgccac gccgcccgcc cgtcgtctgg cagttaaaac ctttgtccgt gagtatgaca
    13681 gcctggtggt ccgggaggcg atcctgcgtg aaattttgcg cggaggacag gtttattatc
    13741 tctacaatga tgtggaaaac attcagaaag ccgccgaacg gctggcagaa ctggtgccag
    13801 aagcgcggat cgccatcggt cacgggcaga tgcgcgagcg cgaactggaa cgggtgatga
    13861 atgatttcca tcatcaacgt ttcaacgtgc tggtttgtac aaccattatc gaaaccggga
    13921 tcgacatccc gacagccaac accattatca ttgaacgcgc ggatcacttc ggtctggcgc
    13981 agctgcacca gttacgcggt cgcgtcggac gttcacatca tcaggcatat gcatggttgc
    14041 tgacgccgca tccaaaagcg atgactaccg atgcacaaaa acgtcttgaa gcgattgcct
    14101 cgctggaaga tctcggggca ggttttgcgc tggcaacgca cgatctggag attcgcggcg
    14161 cgggtgaact gcttggcgaa gaacaaagcg gctcaatgga aaccatcggt ttctcgctgt
    14221 atatggagtt gctggaaaac gccgtcgatg cactgaaagc cggacgcgag ccgtcgctgg
    14281 aagatctcac cagccagcaa acagaagtcg agctgcggat gccgtcgcta ttgccagatg
    14341 atttcattcc tgacgtgaat acgcgtttgt cgttctataa acgtattgcc agcgccaaaa
    14401 cggaaaacga actggaagag atcaaagtcg agcttatcga tcgcttcggc ctgctgccgg
    14461 atccggcgcg taccctgctg gatgttgccc gtctgcgcca gcaagcgcag aaactgggga
    14521 tcaggaagct ggaaggtaat gagaaaggcg gggtgatcga atttgccgag aagaatcacg
    14581 ttaatccggc ctggttgatt ggtttgctgc aaaaacagcc gcagcattac cgccttgatg
    14641 gtccgacgcg cctgaaattt attcaggatt tgagtgagcg gaaaacgcgt atcgaatggg
    14701 tacgccagtt tatgcgtgaa ctggaagaga acgcgatcgc ttaatggtaa atcgggggcg
    14761 tttctgcgcc cccatctgca acatttacaa attctttgca cttccctgca ctatccgaca
    14821 ctgtcaccat ccataattca ggcttatctg tttattacaa taaccttata tttattatgg
    14881 atttttggtg atgatcaaaa cgcgtttttc tcgctggcta acgtttttta cgttcgccgc
    14941 tgccgtggcg ctggcgctac cggcaaaagc caacaccagg ccgctgccgc cagcgggcag
    15001 tcgtctggtt ggcgaaaaca aatttcatgt ggtggaaaat gacggtggtt ctctggaagc
    15061 catcgccaaa aaatataacg tcggctttct cgctctgtta caggctaacc ctggcgttga
    15121 tccttacgta ccgcgcgcgg gtagcgtgtt aacgatcccg ttgcaaaccc tacttccaga
    15181 tgcgccgcgc gaaggcattg tgatcaatat tgcggagctg cgtctctatt actacccgcc
    15241 gggtaaaaat tcggtaaccg tgtatccaat aggtattggt cagttaggtg gtgacacgct
    15301 gacaccgaca atggtgacca ccgtttcaga caaacgtgca aacccaacct ggacgccaac
    15361 ggcaaacatc cgcgcccgtt ataaagcaca gggaattgag ttgcctgcgg tagtgccggc
    15421 tggaccggat aacccaatgg gccatcatgc gattcgtctg gcggcctatg gcggcgttta
    15481 tttgcttcac ggtacgaacg ccgatttcgg cattggcatg cgggtaagtt ctggctgtat
    15541 tcgtctgcgg gatgacgata tcaaaacact ctttagccag gtcaccccag gcaccaaagt
    15601 gaatatcatc aacactccga taaaagtctc tgccgaacca aacggtgcgc gtctggttga
    15661 agtacatcag ccgctgtcag agaagattga tgacgatccg cagctgctgc caattacgct
    15721 gaatagcgca atgcaatcat ttaaagatgc agcacaaact gacgctgaag tgatgcaaca
    15781 tgtgatggat gtccgttccg ggatgccggt ggatgtccgc cgtcatcaag tgagcccaca
    15841 aactctgtaa atcctggata aaaaaaatcc ccgcgacacg gcatgtcgcg gggaaaaaat
    15901 cagcagtagc aggcattaat gagggttaat gcttatttat aaattactgc tgttccatgg
    15961 agggtattcg gaccggttac agaagtaata cggaaagatt ttgcgcccat ctcatccgct
    16021 ttttgcgcca gctgctcttc cagcgatccc agatttgtcc ccgcgttagc actgattgta
    16081 ccgacttttt gttggccttc tggcgttgac tgaacttcga cagccgcaaa gctggcaaat
    16141 gacatggagc ttaaaatcgc cgcagcgatg agggttttta cgtttttcat aatagtggcc
    16201 ttatgcagat gaatgacgtc gcaagcaatt acttaacgat cgataactaa atgatagatg
    16261 tgatctggat cacatacaag atatttttta taacaatcat taattaatgt aaaaatcttt
    16321 aaatttcagt taaatagaaa gcaattattt ttatgggatc tggactggtg aacggaggtg
    16381 attattttta taataacctt tcattcacag tcaccaggta caacggcatc atggcaactg
    16441 actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa agtgttcgac agggatgccg
    16501 cgcttgataa ggccatgaaa ttgttctggc aacacggtta tgaagcgact tctcttgcgg
    16561 acctcgtcga agcgaccgga gccaaagcgc ccacgctata cgcggaattt accaacaaag
    16621 aggggttatt tcgtgccgtt ctcgaccgct atatcgatcg ttttgccgct aagcatgaag
    16681 cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct ggctgactat tttgctgcca
    16741 tcgccaactg ctttaccagc aaagacaccc cggcaggctg cttcatgatc aacaactgca
    16801 ccaccctctc cccagattca ggagatatcg ccaatacgtt gaaatcacgc catgcgatgc
    16861 aagagcgcac tttgcagcag tttttatgtc aacgacaagc gcgcggggaa atcccgaccc
    16921 actgtgacgt gacacatctg gcagaattcc ttaattgtat tattcagggg atgtcgatca
    16981 gcgcacgcga aggtgcatcg ctggaaaaac taatgcagat tgccggaacg actttgcgtt
    17041 tatggcccga actggtgaaa taagtcgcaa aaaaagcccc tcagcctgag cgaatgaggg
    17101 gccaaattgc agagtacagc cttgttatac tttgttattc aaaatcagtt gcccattact
    17161 atctagcggg atctgcgtac ccggatcgcg gtccatgcgg attttgccct gctgatcgcc
    17221 aatcttatag gtcacatcat aaccgagcat tttttctgac ttgtcataca ccgttttaca
    17281 acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga gagccctgga tctggttacc
    17341 ggcatatcca ccacccagcg cccccacaac agtggcgaca tctttaccgc gaccaccgcc
    17401 aaactgatgc cctatcacgc cgccagcaac agcgccgagc accgacccgg taatgcgatt
    17461 ttcatcctgc accggtcgac gatgggtcac tgtgacgttg cgacactcct gacgcggtgt
    17521 tttaaccgtt tccttgattg gggttgcaga aacaacctga gcgtattgcg ggccacgttc
    17581 aaacacgttc agactggcca ctgccgctac gcccagcgca gctgcgacac caatcccgat
    17641 acccgccaac attgatttat tcacgggaac acctccttct ttgcagttat gccaatcatg
    17701 caagcgaagg agaaaagcag ctatcagaca gaagatgaaa agcgcgggaa tggctggaaa
    17761 tacttaatga ttcggaggag tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc
    17821 aggcctaccg ctcgtaccat ttgtccaggg cggatgaggc gtttatgcca catccgccag
    17881 tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac gcggttaata ctccccacca
    17941 gcatcattaa tccggtttta aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg
    18001 agatatatac aaagcgcgca attcgtcctt caatcatcat tgagccgcgc gtcaggttac
    18061 ccatcaggct accaacggtg gagaagttcg acagcgatac cagcgaacca tgatctttat
    18121 actgataatt tttcagcggc ttaccattca tctgcgccag aatgttgttc attgcgcaag
    18181 tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc
    18241 atgacgcgca gtcgccaata gcgtaaatgt ctggatcgcg ggtggtttgc agcgtcggtt
    18301 ccaccaccag ctggttgata cggttagttt caagaccacc gatatctttc aggaagtctg
    18361 gcgctttgat cccggctgcc cacaccatca gatcagcctc aatatattcg ccatctttag
    18421 tgtgcaggcc gccttcatca gcactggtga ccatggtttg cgtcagcacg cgaacgccaa
    18481 gtttcgttag ctcgttgtgg gccgcagcag agatacgtgg cggtaatgca ggcaaaatac
    18541 gttctcccgc ttctaccagc gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt
    18601 agctgtgcag ttgcttgacc gcgttgtgca attcagcgga gagttctaca cccgtcgcgc
    18661 cgccgccgac aatcgcaatg ttcactttgc catttgcgcc caggttggcg gagtatttca
    18721 ggaagagatt cagcatctcc tggtgaaagc gacgcgcctg gtgcgggtta tcgaggaaaa
    18781 tgcagttctc tttgacacct ggcgtattga aatcgttaga ggtgctaccc agcgccatta
    18841 ccagggtgtc ataggcgatt ttacgttccg gaaccagcag ttcacctttc tcatcgcgca
    18901 gttctgcaat agtgattgtt ttcgcttcac gatcaatatc aatgacggaa cccagctgga
    18961 actggaaacc atgattgcgc gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa
    19021 gcgagccagt cgccacttcg tgcagcagcg gtttccacag atggctgtgg ttacgatcga
    19081 ccagcgtaat tttggctttt ttcttgcgtc ccagcttatg ccccagctgt gttgccattt
    19141 ccagcccacc agcaccgccg ccgacaatca caatcttttt caatggcgta gtcaacgtga
    19201 cccccttaaa tttattaacc aattgttaat taatggttat taacatagct cttaattaac
    19261 aacaggttac agcaatgaaa atgttcttcg ctattgagaa taacatgaat ggtgcattgg
    19321 tcataccaaa attgatgtta atcaagtttt gttgctgaaa agataggcga ctttaccggg
    19381 tgagatcaaa aaacagagtt tattgtttat tatttgagca agtccggaag ggcgatatag
    19441 ggtgaagcgt atatcgaggg cgtttggtcg tcctgataag aggcgcatca ggcattgatg
    19501 ctcgaatgtc ggatgcggcg tgaacgcctt atccgaccca cagaatccgg agcgcatcag
    19561 gtaccggcaa ggttacctga tgcgacgagg gcatttaccc gagggttttg aacgctttaa
    19621 tgcgctgtaa atgcggggag atattcttga atttgtgcgt ctgctcttcg tcccagacaa
    19681 tctcgtaata atgatgcagc tcttcagatg tccgctggct gttaagcgct tcatcattac
    19741 gcgacaaaat caccaggcaa cgatcgcgat tcttctcacg gaagttggtc acacacttag
    19801 tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc tcataaggga
    19861 acaaattagg gttgaagatc acctggcgga tatcgcaaag aaaaccaatc cgttccgccc
    19921 agtatccgcc cagaccaacg ccgcaaatca gcagacgctc gtcgacgttc agttgcaaca
    19981 ttttgtccac ttctttgagc agatgctgca tatcatgttt cggatgccgc gtactgtagc
    20041 ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg tggttacccg
    20101 gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat aagccagggt
    20161 tagtgacctg ctttctcttc ctgccagcgt tcatgtaact gattcagacg ggtgctgatc
    20221 gctttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa
    20281 cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac ggccccttta
    20341 cgattattgc agaccaggat catatcgcaa cccgcatcca gtgatgcctg cccgcgttcg
    20401 gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa aatcacgccg
    20461 tcaaaaccca gttcctgacg caaaacggtt ttcagccagt agggagaacc gctcgccgga
    20521 cgcggatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag tttattttcg
    20581 cggattaagg aactgaagac cgacatatct ttagcgcgaa tctccgcttg tggacgtgga
    20641 tcgcacggtg tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg gaagtgtttc
    20701 ccggtcgttt tcattccggc ttcatgcata ccatcaataa accggctggc aattgccagg
    20761 gctttttgtg gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat atgcccgaca
    20821 tccagcactg gcgcaaagct gatatcaata tccatagcga tcatttcgct ggccatcagc
    20881 caacccgcct cttgcgccag tttgccgccc tcttccattc ctgacagcgc agcgaatgat
    20941 tgcgccgctg gcaagcgggt aaaaccttca cgaaaacgct gcacgcgtcc accttcctga
    21001 tctaccgcca ccaccagatg attgcgcgaa gctgcgcgga tctggcgcac cagttcacgc
    21061 aactgggcag gatcatgata gttacgcgta aagagaatca gccctcccac cagcggatgc
    21121 gcaagtattt cacgctcttc cgcgtccagt tcgtaacctt cgacatccaa cattactgga
    21181 cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat cggccagcct
    21241 gataaattgt tgatcgccgg tttgtcgcca gcggtactca aaccaccctg ctttgagcat
    21301 cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat aaatcttcgc
    21361 gcgagtggca tagtcattga ccaattgccg gtgctgttca gtattttcca cccacactgc
    21421 cgccagttcc agcgcgatat caccatctcc ggcatactcc cagtcgatga gttttaaccc
    21481 tgacgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac ttaagcgtaa
    21541 aggccgtggt tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac ccactgtccg
    21601 ccgcgccgga tcgctttgct gccagtacag ttccagtaac ggcaacagcg ttattcgcca
    21661 gccaaaacgt ggttgttgat gtagataata cagcaagcct gccagttcgt tggtatccgg
    21721 caaatacgtt tttacctcgc cgggcagata gtcgactacc atccagtcac ggagatataa
    21781 atgcggcttc ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact ggcgcaagaa
    21841 cgcggactgc ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct ggccctgatg
    21901 ttcaatgaga aaactcccgc cactaagccc actattaaac gtcgtgacgg gatgaaactg
    21961 cgggaaaaag cgcgacagca attcgtcgcg catgatggga ttattgctgc gaaacggcac
    22021 ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg
    22081 gagcgttaac gttgccagag gcgctggagt acagcacgta atgagcgccg acattgcggg
    22141 caatgcctat ggctttacta cgggtaccta aactgtcctg cggcgacaaa cctaactgtt
    22201 gcttagccat cgacagctgc tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg
    22261 catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta cggttgttaa
    22321 cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc atcttactga
    22381 ccatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca ggcggtgccg
    22441 tttgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc gagggcactg
    22501 tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact tcttctaccg
    22561 gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg gtaatcaagg
    22621 cgtagcgact catttttgtc atcaagattc accccttaca aatatagata aagtctgact
    22681 ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg
    22741 gtaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc ataccagtaa
    22801 aaacgataat gaacggtaac gggttcttgc ctttcgttat aaagcgttga ggatgctgaa
    22861 ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc agccaataaa
    22921 gatgacccca tcaccagcga ttgctcatca ttcaccggaa tttccggatg tgaacgacag
    22981 cccaccagca gtaacaacgc cagagacacc agcccaaagc atccttttct catcgttaca
    23041 gacctttatg tgccagcatt ggtcccagcg gacggccacc caacaagtgc atatggatgt
    23101 ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag ccatcttcgg
    23161 caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc agcgcctgct
    23221 catgctcagc tgacacgtcg ttcacagtcg gaatgaggat attcggaatg atcagaatat
    23281 gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc tggtagacga
    23341 tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct gccacgacat
    23401 tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac tgcgagtata
    23461 gagtatggtc gagttaaacc tgctctttca accttaactc acgatttctt aagcaaaaaa
    23521 tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat gcgctgaaaa
    23581 gacattctct gtttggttta cacgtcaaat atggatgcgt atatttctca tttgcattta
    23641 catacaaaat tattcgcaca ctaaatataa ccgctcatca ccctcctccg gcatgagcct
    23701 gacgacaaca taaaccaaga gatttcaaat gctttcaaca caatttaaca gggataatca
    23761 atatcaagcc agcaccaaac catcactact tgccggttgc atagcattgg cactattacc
    23821 ttctgccgct tttgccgctc cagccactga agaaacggtg attgttgagg gttcagccac
    23881 agctccagat gatggcgaaa atgattacag cgtaacgtct acctctgcgg gtaccaaaat
    23941 gcagatgact caacgtgata ttcctcagtc ggtcactatt gttagccagc agcggatgga
    24001 agatcagcag ttacaaacgc tgggcgaagt gatggaaaac acgctgggga tcagcaaaag
    24061 tcaggcggat tccgatcgtg ctctttatta ttcccgcgga ttccagatcg ataactatat
    24121 ggttgatggt atccccacct attttgaatc gcgctggaat ctgggcgacg cactttctga
    24181 tatggcactc tttgaacgcg tagaagtagt gcgtggcgcg acaggactca tgaccgggac
    24241 gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac gcgaccagtc gtgaatttaa
    24301 aggcgatgtc tcggcggaat acggtagctg gaacaaagaa cggtatgtgg cggatttaca
    24361 aagcccactc accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct accagaataa
    24421 cgactcatgg ctggaccgct acaacagtga aaagaccttc ttctccggca ttgtcgatgc
    24481 tgatttaggc gatcttacga cgctttcagc cggttacgaa tatcagcaca ttgatgttaa
    24541 tagcccaacc tggggcggtt taccgcgctg gaatactgat ggcagcagca acagttacga
    24601 tcgcgcacgc agtaccgcac ctgactgggc gtacaacgat aaagagatca acaaggtctt
    24661 tatgaccctg aagcagcggt ttgctgatac ctggcaagcg acgctgaatg ccacccactc
    24721 tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg
    24781 tatgctgatt gggccataca gtaattatgg acctggcttt gattatgtcg gcggcaccgg
    24841 ttggaacagt ggcaaacgta aagttgatgc gctggatttg ttcgctgacg gtagttatga
    24901 attgtttggt cgtcagcaca atctaatgtt tggtggcagt tacagcaaac aaaacaatcg
    24961 ttacttcagt tcatgggcca acatcttccc ggatgaaatt ggcagtttct acaactttaa
    25021 tggcaatttc ccacaaaccg actggtcacc acagagcctg gcgcaggacg ataccacaca
    25081 tatgaaatcg ttatatgctg ccactcgtgt cacccttgcc gatccgctgc atctgatcct
    25141 cggcgcacgt tataccaact ggcgggttga tacgctgact tacagcatgg agaaaaacca
    25201 caccacgcct tacgctggtc tggtgtttga catcaatgac aactggtcga cctacgccag
    25261 ctatacctct attttccagc cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc
    25321 aatcaccggt aacaactacg agctgggtct gaaatcggac tggatgaata gccgtctgac
    25381 caccacgtta gccatcttcc gtattgagca ggataatgtc gctcagtcca ccggtacacc
    25441 tatccccggc agcaacggcg aaaccgccta taaagcagtg gatgggacag tcagtaaagg
    25501 ggtggaattt gaactcaacg gcgcaattac cgacaactgg cagctgacat ttggcgcaac
    25561 gcgctatatt gcagaggata acgaaggaaa cgccgttaat cctaatctgc cacgcaccac
    25621 ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca gagttgacag tcggcggtgg
    25681 tgttaactgg caaaatcgcg tgtataccga caccgtgaca ccgtatggca ccttccgcgc
    25741 cgagcaaggt agctacgcgc tggtggatct cttcacccgc taccaggtga cgaaaaactt
    25801 ctcgttacag gggaacgtca ataacctgtt cgacaaaacc tacgatacca acgtggaagg
    25861 ttctatcgtc tacggcgcac cgcgtaattt cagcattacc ggcacgtatc aattctgaat
    25921 aacacctgta aaaaaggcag ccatctggct gccttagtct ccccaacgtc ttacagatta
    25981 gtggttacgg atgtactcat ccatctcggt tttcaggttg tcggatttag taccgaaaat
    26041 cgcctgaaca ccagaaccag caaccactac gcccgctgcg cccagtttct tcaggccagc
    26101 ctgatccact ttagacacat cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag
    26161 gttagtaatg ttttctttac caccaaatgc agcaaccaga gccggtgcca tttcgctggt
    26221 acctgtcgct tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc
    26281 tttaatcagc acgcggaaga tggtgtagta aacaatcgca taaccgatac cgacgatcgg
    26341 gaacagccac agtttgctgc tgttaccaga cagaacgatg aagtcgatca gaccgtgcga
    26401 gaacgacgta ccgtcacgca tccccagaag aatacagatt gggaatgcca ggcctgccag
    26461 aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg aaggagaact cgatcggctc
    26521 ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc ataataccgc ccactttcgc
    26581 gcggttttct ggtttagcag agtgccagat agcaattgcg gcagctggca gaccgtacat
    26641 tttgaacagg aagccaccag acagtttacc cgcagtcggg tcacccgcca tataacgcgg
    26701 aatgtcgccg tggaaaacct gacctgctgc gttggtgtat tcaccaatct gcatctggaa
    26761 aggtacgttc cagatgtggt gcagaccaaa cggtaccagg caacgttcga tgaaaccgta
    26821 aatgccaaac gcaactaccg ggttctggta agcagcccac tgagagaagg tctggattgc
    26881 agaaccaatc ggcggccaaa tgaaggacag cacaacgcca gtaaagatgg cagccaggcc
    26941 agaaatgatc ggcacaaagc gtttaccggc aaagaagcca agatactcag gcagcttaat
    27001 acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg gagataatcc ctccgagtac
    27061 gccagtatcc gccaggtgtt tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg
    27121 cgcaaccacg gccatggttt taaccatgat gccataggca acaactgcgg ccagcgcgga
    27181 tacgccatcg ttattggtaa agccgagggc gacaccgatc gcaaaaatca gtggcatgtt
    27241 tgcaaagacg gaaccgcctg cttctgccat aacatgcgat acaacggcgg gcagccagct
    27301 gaaattcgcg gaaccgacgc ccagcagaat acctgcgata ggcagtacgg ataccggcag
    27361 catcagcgat ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca taattgagag
    27421 tgctcctgag tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg
    27481 ccgtgtacag ggcatctaag cgccctttat ttattacaca gagtaaaata attcagtgcc
    27541 aatttgtttg acggctatca cgtttcaact tcagatcatt ggagaagttg cacattttga
    27601 caaaacatat gtcacaatat ttcaaacgtg gttattcacc gcccaatttt cggcggtgaa
    27661 ctttactcgt tttagccatt aattacgagc tttaaaaaaa ctcattcaac ggatggattg
    27721 aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta cctgcgccag
    27781 ttcttcaacg gcaacacctt tcaacacagc catgtattct gcaacgtcac gaaccatcgc
    27841 aggttgattc tcttttcctc gatgcggtac cggcgcaagg taaggtgagt cagtttccac
    27901 cagtaatcga tccaggggga cataacgcgc agcatcgcgc agttgctccg cattacggaa
    27961 ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc agtaatttac ccgccgtttc
    28021 tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag
    28081 aattgccagc gtatcggcac gggcgtcacg ggtatggacg ataaccggct tgttcagttc
    28141 acgaccaatc tggatatgat ggatgaagga ctcttgctga cgtactttag tttccggtgt
    28201 gtaataataa tccagcccgg tttcacccag cgctacaaca ccctcttctg ccgccagacg
    28261 gcgtaaatct tctacatcgt agggatcatt ctggttgagt ggatgcacgc cacaagaaaa
    28321 tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt aaataacccg gtaatgttgt
    28381 ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac
    28441 gtccttatgc aaagattcat aatccagacc atcgagatgg cagtgtgagt cgactaaaaa
    28501 catgatgtct ctcttaaaga tgaggaaccg gtagcacaac gcccggttgc aggtaatgct
    28561 caatacgcag taaaagatcg gtgatgagaa gctcgcggtt gatgcctgta acagacatta
    28621 actgttcacg aatgtggcaa acatccccca gtatagcctg caggcgcgag ggagaaagat
    28681 ggtttgccag ttcggcgacc aggcccggca catcaacatt ggtcacctgc gcagcaccat
    28741 gatggcgttt tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc gccggagctt
    28801 gttcatgatt aagggccgct agcagcgaat accagtcgcc cgatggcacg ctatatgcca
    28861 acgcctgaca caatgtttca cgagcctgcc agttatctcc ctgaaacaac gccagtgccg
    28921 cgccaggcga accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca
    28981 cttcgcgtga aagccaggtc acggcgtact gttccggcgg cggcgcaagg taatgtaacc
    29041 gacaacgact acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc
    29101 aggtttctgc tggtggctct tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca
    29161 gtaaggcagc atcggttacc cagacaactt tcgcaccacc taggcgtgcg tgctcattca
    29221 gcttttcggt gacctcacgt accgcatcaa tgcccagcat atttttccct ttttcgggag
    29281 ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac
    29341 cgcaactttt gtggccctgc ggttgttggc agagtaaata acggctcagg gcgtagatta
    29401 aagcatcatc gcccatgccc ggtaacgcct gaatgagtag cgcatggtga cctcttccgg
    29461 cctgatagct ggctaccagt ttttcgaaat caggtcgtaa ccatggatac catctcatgc
    29521 gtccaactcc ttaacccagt gggtcacggt agtgcggatt gcatccatca cggcctccag
    29581 cggctgggtg gcatcaatgg tatgaatact tttatcttgt gcagccagtt ccagatagcg
    29641 ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca atacgatcca gctcgccgcg
    29701 cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg agatagagcg ttaagtcggg
    29761 gcgaaaatcc ccgagaacag catcacgcag tgttgccagc atatgttggt caataccacg
    29821 tcctccgccc tgatacgcct gagtggagag atcgtggcga tcgccaatca cccaggtgcc
    29881 gttagccaga gctggtttga tgaccgtttc taccagttga acgcgcgcgg cataaaacat
    29941 cagaacttcg gctttatcgg taatgacttc atcgcctacc gatttgatat ccagcaccag
    30001 gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg
    30061 gatacccagt tgctcaagcg tctcaaccac cacattacgc gcggtagttt tgcctgcgcc
    30121 ttccagcccc tcaatgacga tatacttact gcgcattttt ttccttaagc actttcagat
    30181 aatcctgcac agacttgtta tggctggcaa gattggtatt aaacgtgtga ccacctttac
    30241 catcggccac aaaatagaga tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat
    30301 ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt aatggtatag gtgttatacg
    30361 ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt ataacgctct cccatcccgt
    30421 aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg taaacggttg ataaataccg
    30481 aggcaacctg atcgcgttca ctggcaacgg cggtttcttt ttcgataatt gatgccatcg
    30541 tcaccagctg atttttatct ttataaggca gaccgtccgc acgcccttcc caggcgctat
    30601 cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa cgcgacatcg gtggtattgg
    30661 cggtatacat ccaggtgtct ggccagaacc aaccttcaat ccactccggg ttttccagtt
    30721 caagtgcctg cgctacggtg gcgtacttat cgtcactcag cgtgtgcttg atatacggag
    30781 cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc ttctaccagt cgcagcggga
    30841 actgtgcttc tttaccgctt tccaacaatt tcagcatctc gcgcacggtc atctgcggtg
    30901 taaagcggta agtcccggct ttaaagtgag aaagatccgg ttcgatacgc agcagccatt
    30961 gaaaaacccg tgggcgattg atgatcttat cagcataaag ctgttcaccg agcgccagac
    31021 gtccggtccc tggcttcagg gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg
    31081 caagatggcg aaccttccag acgcccacac cagcggcgat acccagtacc accagcaata
    31141 acaagataat caataacact tttttcatga ctaattcggg cgctcacaaa gtggggctaa
    31201 atattcatat aacgttgctg acgaaaagga gacatcgcca caggcacgta cggacatcac
    31261 tggcattaac gcattacaaa taaccatctc atctgcctgc aacgcctctt ccagagaggc
    31321 ttgcacttcg acaagctgat aagaggattg tgccagcaaa cggatacaga attgtcgcat
    31381 aatgccgtta acacctgcct gatccagtcg cggcgtataa actacattgc ccttccgcca
    31441 gaacaaatta gccgcacagc attccgtaac ccacccttcg ctgtcaagga ccagcgcctc
    31501 atcagcgttt gtctgctcaa gatgagagcg gatcaatact tgctcaagcc gattaagatg
    31561 tttaatacct gcaagatgag gattgcgccc cagccgcacc gggcttagcg ccaacgtcat
    31621 cccttcgtta cgcaaacggt cgtaatgtgc aggataaacc gtaacggaga gaatccgcgt
    31681 tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac
    31741 tttcagcaca ccattttgct gttctgctgc cagcgttttc atctcttgtt caagctgagg
    31801 ccagaagtca cagaaaatca tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc
    31861 cgataacaaa ctgactttac cgtcgataac tctggcggtg gtaaaacaac catcaccaaa
    31921 ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta taaccgttaa ttaagaacat
    31981 accggctcct tatcagagaa ttgttagtgt ggcagcatgt tcactacgga acaagtcgga
    32041 ataaaagcta agaaaaaagg cccgcaagcg gaccttttat atgggtggaa aatgacaact
    32101 tagatctttt taaagatcaa agaaccatta gtgccaccga agccgaagga gttacacagc
    32161 gtgtattcca ttccgctgac ctgacgtgct tcatgcggta cgaaatccag atcgcaacct
    32221 tcatccgggt tatccaggtt gatggttggc ggaacagcct gatcgcgcag cgccaggatg
    32281 gagtagatag attctactgc acccgccgca cctaacaggt gaccggtcat agatttcgtg
    32341 gagcttacca acacacggct tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca
    32401 gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct
    32461 tcaatgcctg catcacgcag agcatttgcc attgccagag ctgcgcctgc gccattttct
    32521 ggcggtgacg tcatatgata agcatcgctg ctcataccaa agccgacgag ttcagcgtaa
    32581 attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa gtaccagcat accggcacca
    32641 tcgcccagta cgaagccatc acgctcttta tcccacgggc ggctcgccgc ttgcgggtta
    32701 tcattgcggg tagataatgc acgtgccgcg ccaaaaccac caacgcccag cggcgtactg
    32761 gctttctctg cgccacctgc aaccatcacg tcagcatcgc catacgcgat aatacgcgca
    32821 gcatgaccaa tgttgtgcac gccggaagta caggcggtcg cgatagagat gcttgggcca
    32881 cgcaggccat acatgatagt cagatgacct gccaccatgt tcacaatcgt tgacggaacg
    32941 aagaatgggc tgatcttacg tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc
    33001 agtccgaggc cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc
    33061 gttatttcaa ggccagaatc ctgcatggcc tgaacgccag cgacaattcc atattgaatg
    33121 aaggcatcca tcttgcgctg ttctttgcgc gagataatgt cctcacagtt aaaatccttt
    33181 actaagccag caaatttcgt tgcataggcg ctagtatcga aatggtcgat taggctgatg
    33241 ccactctgac cggcaagcag agctttccag gtagactcta cggtattgcc gacaggagac
    33301 aacatgccca gtccggtcac aactacacga cgcttagaca cgtttgtcct ccagggaggg
    33361 aaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga ccgcctggag
    33421 atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac ggtggtgatt
    33481 ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc cattaccagc
    33541 tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt gttggtaact
    33601 tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc ttcgatagtg
    33661 ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt agtgtataaa
    33721 atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac gttattttga
    33781 ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata accacgcaga
    33841 ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc
    33901 acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg
    33961 ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag gatacccgca
    34021 cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg
    34081 tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa gccgatcaag
    34141 cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc aaccacagaa
    34201 ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac cgcttttgac
    34261 agacggaaaa cagatgaaag gttggtttcg ataatttcgt tccactcttc atctttcatt
    34321 cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc cacttcacca
    34381 aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt cacattcaac
    34441 atcagacctt tgccgttggc acctaaataa tcgctgatcg cctgagcgcc attttcactg
    34501 gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca
    34561 attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat gattttcctc
    34621 ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg
    34681 gtgtcgacaa tgcgtttcgt caggccagta agcaccttgc ccgggccgac ttcatagaga
    34741 tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac cgggttatac
    34801 aactgacgta ccagtgcgtc acggatggca tcaccattgg tttcgcattt cacatcaacg
    34861 ttattcacaa cagaaactgt tggtgcgtta aaggtgattt tcgctaattc tactgccagt
    34921 ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg taacggcagc
    34981 gcgcgttttg cgccagcagc tttacaggca gcgccagcac gctcaaccgc ttctttatga
    35041 ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac ctgaccttct
    35101 gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat tgccgccata
    35161 gcgccagtgc cttccggtac ggcttcttgc atgaacttac cgcgcatctc aaccagacgc
    35221 accgcatcag cgaaatcaat cacgccagcg caaaccaggg cggagtattc ccccaggctg
    35281 tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg atacagcgca
    35341 acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc ttcagctggc
    35401 ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc agcaaacgtt
    35461 tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt ttgagaaccc
    35521 tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta gaaacgaacc
    35581 agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc
    35641 ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc cgcagaggta
    35701 ttaccgtggc gatccagcgt caccacgaca ttgtccatag acataccgag ttttttcgcc
    35761 gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag ttgagaacgg
    35821 tcaagattat ttgccgccag cgtctcatca acgatgtgcg ccagttccgt taccgcaacc
    35881 ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt cacacggtca
    35941 gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg catgcagatg ggtggagatg
    36001 attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc aaaaataata
    36061 atagtcccac gatcggttgg atcgcaggtg cgcgccagta catcggaacc gacgaccaga
    36121 gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa
    36181 cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccaa catgctttga
    36241 atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg
    36301 ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc
    36361 atggttgaaa cggtttcgtt tggtgcggca atgtggcgtt cgcggatacc ggtacgagtg
    36421 acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt
    36481 tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca gtcacttttc
    36541 ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct accgcagagt
    36601 tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt ccaggcgagc
    36661 ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc
    36721 aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca atcctaacag
    36781 acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca ggctcttttg
    36841 tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac cctgagattt
    36901 cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaacgtga catttcctgt
    36961 aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg cttcaagata
    37021 gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc gaatactgtc
    37081 gagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat tgggaatttc
    37141 caccacctct tcagccagaa ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc
    37201 acaatcgacg ttggccccta aatcgaggac caccgttttg cccttttgct gatgtggtaa
    37261 taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata attttgccag
    37321 ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac cttctttcac
    37381 cagctccagg gccatacgca ttgaactccc acgactggcg cggatagctt gcgaaggccg
    37441 ggcatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac gttgttcaaa
    37501 gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag
    37561 ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc
    37621 ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt
    37681 ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca gacgcgtgat
    37741 tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg
    37801 tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag cgcgtcatgg
    37861 gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc catggacctt
    37921 actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt acgctttaag
    37981 ctggctaata cggcaaatgg gtttggcttt tgcgcttctt caggcagttc accaaagacc
    38041 atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac cggcaaggcg
    38101 aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc gttaacctca
    38161 atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa
    38221 caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt
    38281 accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat agcgaacgac
    38341 atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg ctcaacctga
    38401 tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga
    38461 gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa cgtcatagag
    38521 tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta
    38581 ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga agcgcatagt
    38641 ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct aaacttattt
    38701 tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat
    38761 gtgcagcacc agaggtcgac gaaaccccac gcagcgacga atcaccgcga cagttggtgc
    38821 ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat catttaatta
    38881 ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag
    38941 aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc tatactggac
    39001 tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct tttgacgtcc
    39061 atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaggag catcccctgc
    39121 actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag cgtttagagg
    39181 gacgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag atgttacgtc
    39241 gggaagggaa aaacccgctg atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc
    39301 cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca accgcccatt
    39361 atcaggctta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa
    39421 ccttcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg ggtaaacttc
    39481 aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt gagctgtctg
    39541 tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc atactgtgta
    39601 tgcacacgga tctgatgagt acgtccggtt accgggctac aacgcaccag ggtggcaaat
    39661 gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg actgacacgc
    39721 acgatacgtt cgccgctttg cagaatattt ttcaataacg gcgcttgaac gctcttcaca
    39781 tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcattcc tttttcacgt
    39841 aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa cacacctgaa
    39901 gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg caacgcccgc
    39961 aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt accggaaggt
    40021 ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag cgccgccacc
    40081 ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac cggtgggata
    40141 cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt gttcacccgc
    40201 acttcacctt tacgcaaaat acggtaaatc atactttttg gtacgccttt caattgggta
    40261 cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac aatttttacg
    40321 gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt cgcgccactc
    40381 atttttctat gcttatattt agttttacac cttattactt cactacgtga tcactttatt
    40441 gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa
    40501 atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg
    40561 tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga ccgatcatcc
    40621 acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg cgggttgcag
    40681 tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt ctttgataat
    40741 tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt tgagctgacg
    40801 ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac ccggaaaggc
    40861 gttactttgc ccacagctta gtcgtcaatg taagaataat gagtaagtta cgatgaaaag
    40921 aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg
    40981 tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa acatctacaa
    41041 aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt acggcgctga
    41101 acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg ctaactacag
    41161 tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca
    41221 gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta tcagtctggc
    41281 gggtagctat ctggttctga tgccgaacaa cccgcgcgcg ggtggcattt ctcgccgtat
    41341 cgaaggcgac gaccgtaccg aattaaaaga agcactggcg agccttgaac tgccggaagg
    41401 catggggctt atcgtgcgca ccgctggcgt cggcaaatct gctgaggcgc tgcaatggga
    41461 tttaagcttc cgtctgaaac actgggaagc catcaaaaaa gccgctgaaa gccgcccggc
    41521 tccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg attacttacg
    41581 tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg cacgtcagca
    41641 tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca ccggcgagat
    41701 cccgctgttc agccactacc agatcgagtc gcagatcgaa tccgccttcc agcgtgaagt
    41761 ccgtctgccg tccggtggtt ccattgttat cgacagcacc gaagcgttaa ccgccatcga
    41821 catcaattcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa
    41881 cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat
    41941 tgttatcgac ttcatcgaca tgacgcctgt acgccaccag cgtgcggtag aaaaccgtct
    42001 gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt ctcgctttgg
    42061 cctgctggaa atgtcccgtc agcgcctgag cccatcactg ggtgaatcca gtcatcacgt
    42121 ttgtccgcgt tgttctggta ctggcaccgt gcgtgacaac gaatcgctgt cgctctctat
    42181 tctgcgtctg atcgaagaag aagcgctgaa agagaacacc caggaagttc acgccattgt
    42241 tcctgtgcca atcgcttctt acctgctgaa tgaaaaacgt tctgcggtaa atgccattga
    42301 aactcgtcag gacggtgtgc gctgtgtaat tgtgccaaac gatcagatgg aaaccccgca
    42361 ctaccacgtg ctgcgcgtgc gtaaagggga agaaacccca accttaagct acatgctgcc
    42421 gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg
    42481 tccggaacaa cctgcgctgg caacctttgc catgccggat gtgccgccag cgccaacgcc
    42541 agctgaacct gccgcgcctg ttgtagcccc agcaccgaaa gctgcaccgg caacaccagc
    42601 aactcctgca caacctgggc tgttgagccg cttcttcggc gcactgaaag cgctgttcag
    42661 cggtagtgaa gaaaccaaac cgaccgagca accagcaccg aaagcagaag cgaaaccgga
    42721 acgtcaacag gatcgtcgca agcctcgtca gaacaaccgc cgtgaccgta atgagcgccg
    42781 cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg
    42841 tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcaag ctgaggtaac
    42901 ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta gccgccgccg
    42961 taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc
    43021 tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg
    43081 tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc
    43141 ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag ctccacgcac
    43201 agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac agcaagaaga
    43261 gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg
    43321 tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca
    43381 gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg
    43441 gatccgctat ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga
    43501 agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg tcgaacctgt
    43561 tgttagcgcg ccagttgttg aagaaatggc tgaagtcgta gaagcccccg taccggttgc
    43621 cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac
    43681 tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag tcgcagaaca
    43741 cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag ttgccgaaac
    43801 tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc cagtcgtcgc
    43861 tgaagtcgca gcagaagttg aaacggtagc cgcggttgaa ccagagatca ccgttgagca
    43921 taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc cggaggcacc
    43981 gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg
    44041 tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata
    44101 attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc atctttgaat
    44161 caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa
    44221 aaatgcccgc cccggcgatc ccagagcagg cagacaaaac atacccagtt tcatgatatg
    44281 ttttaaagcg agcttatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg
    44341 ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc
    44401 agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta
    44461 atgaatccag cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga actgtaagga
    44521 cattattcag cgagtttttc agtccacggt tggttttatc caatgccgca gcggcgattt
    44581 ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc tgcgatggcg ctatccagca
    44641 tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg ttactggtaa
    44701 tactgtcgaa aattttgtcg cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct
    44761 gttgcctaat actttctgct ccaccgacgt atttcccttt ctcttcgcta aacggcgcag
    44821 tctctgtttt ataaccggca aaaatgtagc gcccgttacc gtcagtggtg ttcgccagat
    44881 tcagcaactg gtcacgaagc ccctggatat ccgtagccag cgaggcccgg tcatcgtcac
    44941 tcaaagtgcc attgctggcg tagacaattt tttcctgagc attctggata gcagtggtga
    45001 cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta cgcgccagcg
    45061 tgtactggct gttttgcgcc tgtgcctggg agagaacaac cgcttgtgat gctgcaatgg
    45121 gatcgtcaga agggttaacg actcgcttac ctgtcgacat ctgctcgccg tacttcatcc
    45181 attccgcctg agaattggtg atgccacgca tgttttgctg gtacatcatc tgggtactga
    45241 aacgcatctt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt
    45301 ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc
    45361 ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt
    45421 accttgcgtg gcgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa
    45481 cgaggcataa gcgtcgttaa aggatttcgc accgcccacc gttttactgt tgctttgcag
    45541 atccagcagg gcctgaccgt tgcggttatc gctatcaccc gcatcttctt cgctcgccat
    45601 cgctattttc gcttcgtcgg tgattaatac atccatgttg acgatagcgt cacttactgg
    45661 tttcagcgtg aagctgtcgt taacagcagg cgttcctgta aacgtcaact ccagaccatc
    45721 aaatgccact ttaccgttgg catccggcgt caccgtaaaa gtggtattac tggcaaggcg
    45781 ggtgacctgc cactgattat tatcgaacga gattttgtaa tcagtcgcca gtaccgcgga
    45841 ggcatcagtt accgtggcac cgatcgcaac gtcacctgtg tttttcgtgt tttgcagaac
    45901 cgcgggctta ccgatagcaa agaaatcttc accagcatcg ccgttggcat caaatccggc
    45961 tttgtgttgg ctgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg
    46021 cgtctggtcc agatcctgag aacggaatgt cagaatgccg cccagcgccc cggtattcag
    46081 taatttctcc gggatctcaa tattgcctgc cgtcccatca acgtaagcga cagtcgtacg
    46141 agaagggtcg gcgctggaag gaactgccgc cagttgccgc gccgtacttc cctgaaccag
    46201 tgagtaacca ttggccatcg tgatgttata agtaccgcca tcctgaacgc tgacttctac
    46261 gccaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca gattgttagg
    46321 tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc tggcaatttg
    46381 tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga cctgtttgtc
    46441 ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt ctgatttccc
    46501 aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc accagcgttt gcaggctggt
    46561 gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca tattgtcgat
    46621 tttcgacatc tgctcatagc gggcagtcag gccgctactt tgcgtctgcg ccgcacgtaa
    46681 ctggttggta ataaaagcat catactcacg ctgcacacca gaaacgtaga caccattgcc
    46741 aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt
    46801 atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg ccgcctgggc
    46861 cgcgttcagt ccgctcatgg cgttattgat caagctggac atggaggttc cttataagcc
    46921 ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca gaacagatta
    46981 tcaatgttca tgctgtaggt tttgctcacc ttgtcgctta tcgatttcat ctgctggatc
    47041 atgttggtga gtttacgggc atagtgagga tcggtagcat accccgcgtc ctgtagggcc
    47101 tgcgccccct gctccgcact cgcggcggtc gtcacggcgg cgtagcgcgg gttacgtgtt
    47161 aacagcccaa cgtaatccga caatgcttcc agatacgagc tgtagacccg aaacttcgct
    47221 tttactttct tcgcttcgcc gttttcatat tcagtcgtgg tgatttcagt aactggccct
    47281 ttccagttgc cagaggcttt gacaccaaac aggttatagc tcggctcgcc gttttcgcgg
    47341 cggatttgcc gttgccccca accagattcc agtgccgcct gagcgaggat caaatgatgt
    47401 ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc gaggaatgct
    47461 ttactgtcac ccggcagcga atcatcgtag ttacgtggca cggccttttg caccagctgc
    47521 gaaagcgcct gattttgata acgcaccaca gtttcgagcg ggaatttcat cggtgctgct
    47581 ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat catctctgca
    47641 agccccagac ctttgcccgc cgtcatctgt tgggcaatct gctggtcata catactggta
    47701 tacaggcgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt
    47761 ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg gatatttgcc
    47821 gccggatctt cgccctcttt cgcctttagt tcgttgagtg attgcgcatc ccaggccgca
    47881 cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc cgcagacatc
    47941 ccgcactttg cattgattgc agtatggaca tcagatccat cggcgtagcg cccagcgcat
    48001 tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc
    48061 cgctctggcg taaatcgatc tgcgtttgtg gagtaaccac agtctgtcca ccaccaaacg
    48121 gtgtatctgg ctggctgaca ttggcctgac gattgactgt tactgagaga tttccctgcg
    48181 ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac caccgaaccg gtgcgcgagt
    48241 taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg atatctgcaa
    48301 ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta
    48361 acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt tgctgcgcca
    48421 tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca aactggctgg
    48481 gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt tggttcacct
    48541 gaacactgct accgccagcg gaggctcctg cgccgccaac cagaatattg ccctgcgcca
    48601 gcgcatacac ctgactgtca acgcccttaa gcggcgtcat caacaacgta cctccacgca
    48661 agcttttggc atttcccatg gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa
    48721 acggaggaag tgacgctgtc accattaccg cagcgacgtt ttttagctgc atattggtgc
    48781 ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg
    48841 gcgtctgggt tgtctggtca ccggtgccat ccagccccac caccagacca tagccaatca
    48901 gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca gcctgagccg
    48961 ccgtcgtaac cagtagaaga attaatgcag agagaaattt aatcaccaca gcctcactta
    49021 cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg cttcgttaat
    49081 gtagccattg cctacgtatt caatgcgcgc atccgccacc tgagtagacg gtacggtatt
    49141 gctgccgctg atagtgcgtg gattaaccac gccagagaag cgaataaatt cggtaccctg
    49201 attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca gtacctggtc
    49261 aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc cctttccgtt
    49321 gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa
    49381 atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg cattcgcaga
    49441 ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat cgccaatatt
    49501 gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct gagcagactg
    49561 gaaaatagaa ccgttggcga cgggcgtcgg cccgggaacc ggttgtgcac tggtcgcccc
    49621 ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca ccaacaagct
    49681 ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca aaaatcagta
    49741 aaccggtgaa cctgccaccg gttaagcctt agagttgcgt cagtttttgc agcatctgat
    49801 cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga atcatattga
    49861 ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac agcagtcccg
    49921 cgccgttcag gcccggcgtg ctttcgttcg gtgcaccaga ggattgcgtt tcggtgtaga
    49981 ggttttcgcc aatgctctcc agcccggtgt cattcataaa ggtggtgaga ttgagctgcc
    50041 caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca tcacgaccga
    50101 tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga aaaccaccag
    50161 ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg
    50221 aaccatctgg caacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt
    50281 tgttggtctg cgacaggttt ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc
    50341 ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct
    50401 ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag
    50461 tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc
    50521 ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaag
    50581 cagagcagaa gcacgcgaca gtgtgaattt cccctcaccc taaccctctc cccaaagggg
    50641 cgagggaata gtccgcgcgc ttttattgca atgtcatcaa tcgttcaatt aactcatcga
    50701 cagcagttgg ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat
    50761 ttcaaaacgc cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact
    50821 gccttccaga acccccgaca tcacacgcaa ggttggatct gcctgcagta tcggcccacg
    50881 cgtggcctgg ctttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgtt gcacttcgct
    50941 gcccgtggct ttcaccagtt tcagacgccc cactggcgca accgtatttg ccggatcgcc
    51001 cggattgagc gccgagattg tgccatcggc agcaatagtg atttccgccc cttccggcac
    51061 agcaattggc ccagcctcgc ctatcaccgg atgcccctga attgtcagtt gcccggtggg
    51121 atcaacctga atgctgccat tacgcgtata cccttcgcta ccgtcagcgg actgcacggc
    51181 cagccagcca tcctgctgca atgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc
    51241 gggcgtcatg tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag
    51301 cccttccacc ggcactgcac gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc
    51361 attggccaga ttactggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat
    51421 cgcggtataa attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca
    51481 gcgtgttgag gatctggtcc tgggttttga tggtctgggc gttagactga tagttacgct
    51541 gggcaacgat catattgacc agttctttac tgagatcgac gttggacgct tccaacgcac
    51601 cgttggtcag ggtgccaaag ttgcccgtcc cggctgtccc caacagcgcc acgccagaag
    51661 attgcgtcgc agaccagacg ttgtcgcctt cggatgccag cccttcgttg ttggcaaagt
    51721 tcgccagtac aatctgcccc agcagttgtg tttgttcgtt ggaatagttg ccgacaaccg
    51781 taccgtcatc attgatttga taactcacca gatcgcccgg tttgtagcca ttctgggtgg
    51841 ttgccacaat attgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa
    51901 acgtggcggg atctgcgccg ttaattgcgc cagtggtaat attctctgtt ggatttgagg
    51961 tcagaacgcc attggcatta aacaccagct tcattgcagg ctcggctgta cctgttggat
    52021 cactgctatc ctgggtgtag acctgccagt tattatcccc ggtcttcaca aagtagacgc
    52081 tcatgtcatg agcattaccc tgactgtcga aaacagtcac cgaacctttt ttgttatagc
    52141 tatccgcatt gctggcgctg aatggcgtaa cagtaggaag cggatcactg gaattcaggt
    52201 tgatctgcat cgatgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa
    52261 tattggtcgg attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaacccg
    52321 tcagctgtaa accttgcata ttcaccaggt tacggttttc atccagctta aattgtccgt
    52381 tacggctgta gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga
    52441 tagcaacgtc cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga
    52501 taccggcaac ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg
    52561 ccgtgcctga tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga
    52621 ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct
    52681 gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc
    52741 cgagatccag cgtattaccg ctgttgccgc ggatcacacc ctgcaccaga gcaaactgca
    52801 gcggctgggc aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac
    52861 ctttcggcgc agtgctgccg tcagtcaacg taccgtccca ggtgaaactg tgaactccgg
    52921 cggtcagttc accaatatca atggtgcgca caaccgcacc atttttatcg gtgatggtgg
    52981 ccgtaacttt gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc gtggtcacag
    53041 ctccttcttc actgccggtt cccgcaagaa cagtggtgcc ggggatcatc acgccgtgac
    53101 cgatcaggtt actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga
    53161 gtgtggtatt gagtttttca atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca
    53221 gctcgttgtt ttccattgga ttggtcgggt cctggttttt cagctgcgcc accagcaaag
    53281 tcagaaaact gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg
    53341 tactgacgcc ggtatttgtc ggatcggtgg tggttaccgc aatggacata gctttctcct
    53401 ttattgaccg agcgtaaggg ttttcagcat catgcttttc accgtgttga gcacttcaac
    53461 gttggcctga tagctgcgtg acgccgacat ggtgttaacc atctccccga cgacatcaac
    53521 gttcggcatt tttacgtagc cctttgcatc tgccagcgga ttacccggtt cataaaccag
    53581 tttgtccggg gcctgacttt ctataacatc ggcaaccttt acgccgcctg tcgcagcacc
    53641 tggtgcagcg ttaacctgga ataccacctg ttttgcccga tatggctgtc catcaggacc
    53701 ggtcacgcta tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc
    53761 agttaacgcc gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct
    53821 ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta
    53881 ttgcaggctg ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc
    53941 aagcgaaggc tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg
    54001 cgccgggatg tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc
    54061 acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg
    54121 ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag
    54181 cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt
    54241 atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc
    54301 aaaatgggtc gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta
    54361 gaaatggctg ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact
    54421 gttcagtccg ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc
    54481 ccaactcgcg ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact
    54541 accgccatgc gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt
    54601 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac
    54661 aggaaattat gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa
    54721 tgtcaaacta aaacgcggac ggctggatac cctgccaccg cgtacggtgc tggatatcaa
    54781 tcaacttgtt gatgccatta gcctgcgcga tctatcaccc gatcaaccta tccagttaac
    54841 ccagtttcgc caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg
    54901 tgatgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa
    54961 tgcgcgggtg cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa
    55021 tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    55081 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    55141 gaagcctgta agcaccgttc aaccgcgcga aaccactgac gcgccggtaa cgaacagccg
    55201 ggcggcaaaa acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa
    55261 actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc
    55321 gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga
    55381 agcgcagcaa gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc
    55441 agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct
    55501 ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc
    55561 tggcgacgct ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg
    55621 ttgatattag tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga
    55681 atcaacataa cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa
    55741 tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacaaccc
    55801 atcgcggcgg taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga
    55861 atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt
    55921 gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag
    55981 ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac
    56041 atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta
    56101 aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga
    56161 ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat
    56221 tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa
    56281 taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca
    56341 attaacgccc gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc
    56401 gaaacggtta acggacctga aagaatgccc aacgcaaccg cactcggcag cgccaacagg
    56461 aaacaaagac gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca
    56521 aaacttttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggac
    56581 ggaaactcca ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac
    56641 gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc
    56701 ggtcccatct gtttcaccac gcgcattgct ccggcatcgt ggaagttaat gcgcggcagg
    56761 accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg
    56821 accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc
    56881 gcgaaaccaa tcatgctgat gttaagcagt gttggagcaa acgccggaat cgagaagcgg
    56941 ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga
    57001 aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct
    57061 ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc
    57121 agcgcgagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc
    57181 tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca
    57241 aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc
    57301 cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac
    57361 atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccag
    57421 acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctggatt
    57481 atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat
    57541 cttgtcaacg atacgttgcg ccagcacggc ctgttcgcca gcggtttgcg gaactgtctg
    57601 attttgcaca cattcaatga agtgccgcgc acagccgaca aatccacgtt gttcaagagt
    57661 gctctgccaa ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct cacgccattc
    57721 gcgcatatcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg
    57781 acttccggca cggcgatgca tacaggtggt gatctgcaaa ggaccggccg aaaaatggtg
    57841 ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc
    57901 tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt
    57961 gaaataaaga tcgtgtggcc cgacactatt gctacgatgt ttatccattc ttagcgaagc
    58021 tgcggtagcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc
    58081 gaccatcaac gtcagttttt tacgcgccgc cagttccacc agccgttcag catcgcgcag
    58141 attttctgcc agcggtttat cgacacagac atgtacaccc gcattgagta acgtactgac
    58201 cacgtcaaag tggctggcgg tgctggaatg cacaaaaacc gcatcgcaac tggcggcaag
    58261 gctggataac gaatcggcat aaggaatgcg ccagctttca caaattggca gggctttcgc
    58321 gcgcgtaggc gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa
    58381 ccacgctttt tgcgcaatgc cacctaatcc cactacgccg atacgtaatt ttttcacttt
    58441 aatctcccag atgggccagc aacgaatcaa gacgctgttt cagttctgcc acttcgattt
    58501 ccagggcttc gacgcgggcc tgtaaatcac catcaaccgc gttcgacata tccgtcaccg
    58561 ccggctgatc ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg
    58621 gttcgcgggc cagacgcacc acaaaaggac catcttcgcg atttgccagt tgttccagcg
    58681 tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt
    58741 cacctggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact tccgctgcgc
    58801 tcagtttcag atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat
    58861 taccaaaacc gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct
    58921 gcacttcgga ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg
    58981 tgactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca
    59041 agcaaccaat cacgcgagct tcaagggcag taagttgata tttcatgaga ttctccttag
    59101 cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca
    59161 tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa
    59221 tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg
    59281 cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat
    59341 ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccacgaac aacattggaa
    59401 aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct
    59461 gaaccttgtt tatgaaattc gttaatcatc cccagcctgg cctgccagcc tgatggataa
    59521 cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa
    59581 taatccgcaa gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg
    59641 gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc
    59701 gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag
    59761 tccaaaatgc cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac
    59821 gttttgataa aaaaatattg ttcccgaagt gatgggaaaa gtcttagcgg caatgcagaa
    59881 tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg
    59941 aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt
    60001 gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc
    60061 ggtattgctc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca
    60121 attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc
    60181 ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc
    60241 tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc
    60301 cgtccacaac aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccggtgcg
    60361 gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc
    60421 acaggggccg ttttgtttgt gctgtgtgcg gcgttcaatg cgtggttgtt accggcatgg
    60481 aaactctcca ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag
    60541 cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg
    60601 ctgatgctgc caattatggt caacgacgtg gctggcgcac cctctgccgt taaatggatg
    60661 tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa
    60721 aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg
    60781 atgccggtgg gcatggtcag cggcctgcaa caacttttca ccctgatttg tctgttttat
    60841 atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca
    60901 agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt
    60961 ggttatatcg gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt
    61021 ccgtggatga tgctgggcat tattggcatc ttcactttcc ttgcgctggg ttggcagttt
    61081 agccagaaac gcgccgcgcg tcgtttgctt gaacgcgacg cctgatttga tactcccccc
    61141 tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac
    61201 atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc
    61261 cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt
    61321 caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggac
    61381 atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct
    61441 gccattgaag agtggctgcg caaggtcaat ggctacgcaa acaaactgct gttgccgcgt
    61501 tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac
    61561 aagaaagagg ccagcgcggg taatttagcc gacctgctgg cccactctga cggtctgatt
    61621 aagaatatca gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg
    61681 aatggcgaac tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg
    61741 gtagccggaa tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag
    61801 acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt
    61861 attcgcgggt tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca
    61921 tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt
    61981 tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca
    62041 atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa
    62101 agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca
    62161 aattggtcgc gaagagccga ataaggttac cctaaccgga gacaccaatc tggacatgaa
    62221 ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt
    62281 tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca
    62341 accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg
    62401 caattacttt aaccagcaac ctgcttacat cctgcgcgaa gatggcagtc agggcgaagc
    62461 aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt
    62521 tacggattaa tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt
    62581 ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac
    62641 tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga
    62701 tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctattgt
    62761 aatgcccaat ctggctccgc ccgtgaccac tgttgaggct gccgtggcgt atcgccagcg
    62821 tattcttgat gccgtacctg ccgggcacaa tttcacccca ttgatgacct gttatttaac
    62881 agattcgctg gatcctaatg agctggagcg cggatttaac gaaggcgtgt tcaccgctgc
    62941 aaaactttat ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc
    63001 aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga
    63061 agtgacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat
    63121 ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa
    63181 agatgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca
    63241 gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg
    63301 tttacccatc ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg
    63361 ttttaatcga gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag
    63421 cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt
    63481 ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca
    63541 gttctatggc ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt
    63601 tgctgaaagc atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt
    63661 acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct
    63721 gtataaataa ccagtatatt caacaggggc tattatgcga attgaagtca ccatagcgaa
    63781 aacttctcca ttgccagctg gggctattga cgccctggct ggcgaacttt cccgccgtat
    63841 tcagtatgcg tttcctgata atgaaggcca cgtatcggta cgttatgccg cagcgaataa
    63901 tttatcggtt attggcgcaa caaaagaaga taaacagcgc attagcgaaa ttctccagga
    63961 aacgtgggaa agcgccgatg actggtttgt cagcgaataa tatgcagtga tttttgtttt
    64021 tgccgggtcg ccccggcttt tttattttac ttacaaattt tgagtacaaa atttctacac
    64081 ttcctcaaaa taattgcaca atatgctaaa aaaatatgaa tgacatgctg ttttattgtt
    64141 catataaatc ttaaaagcag aaatttatct gttcccccct ttaaaaattc ggtgaatacc
    64201 cttacttatt ggtatactga agttgcttca gataaaaacg cattgaacct cgaataacgt
    64261 tgtctagtaa cacgaattag ggggccatga tggaaaaaaa taatgaagtc attcagactc
    64321 atccgctcgt agggtgggac atcagcaccg ttgatagcta tgatgcgctg atgttgcgtt
    64381 tgcactacca gaccccaaat aagtccgagc aggaagggac tgaagttggt cagacgctct
    64441 ggttaaccac tgatgttgcc agacaattta tttcgatatt agaagcagga atcgccaaaa
    64501 ttgaatccgg tgatttcccg gtaaacgagt atcggcgtca ttaacatgac atgaccatcc
    64561 acccaaaagg cacctcaccg gtgcctttac catttctggg ttgtatatct gcattcagtt
    64621 aattactctg cttaaaccat gagagcagag agaaaaaaat gaaatacgat ctcatcatta
    64681 ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc aacccgcgcc ggtttaaacg
    64741 tgctaatgac cgacgcccat atgccaccgc atcaacacgg cagccaccat ggcgatacgc
    64801 gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt cccgctggtc ctccgcgcgc
    64861 aaacgctgtg ggatgaactt tcccgccaca acgaagatga tcccattttt gtacgctctg
    64921 gtgtcattaa cctcggcccg gctgactccg catttctcgc caacgtcgcc cacagcgccg
    64981 aacagtggca actcaacgtt gaaaagctcg atgcgcaagg gattatggcc cgctggccag
    65041 aaatacgcgt cccggacaac tacatcggct tatttgagac tgattccggt tttttgcgca
    65101 gcgaactggc gattaaaacc tggatccaac tggcgaagga agcgggctgt gcgcaactgt
    65161 tcaactgccc ggtcaccgta attcgtcatg acgatgatgg cgtaactatt gaaacggttg
    65221 acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg aacatgggta aaagacctgc
    65281 tcccggagct gcctgtccag cccgtacgta aagtatttgc ctggtatcag gccgatggcc
    65341 gctatagcgt gaagaataaa ttcccggcgt ttaccggtga actgcccaat ggcgatcaat
    65401 attatggttt tccggcagaa aacgacgcgt tgaagattgg caaacataac ggaggccagg
    65461 ttatccattc agcggatgaa cgtgttccgt ttgcggaagt ggtcagcgat ggttcggaag
    65521 ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg ctgcctgtac ggcgctgcct
    65581 gcacctatga taattcgcct gacgaagatt ttattatcga taccctaccc ggccacgata
    65641 atacactgct cattaccggc ctgagtgggc acggttttaa atttgcgtca gttttagggg
    65701 aaatagctgc cgattttgcg caagacaaaa aaagcgattt tgatttgacg ccattcaggc
    65761 tttcccgctt ccaataatca tcattacggc ctccagaatg aggccgtttt tacaggagta
    65821 tttaatgcgt cggctgttgc actatctcat caataatatt cgcgagcatc tgatgcttta
    65881 tctttttctc tggggattgt tggcaattat ggatctgatc tatgtgtttt attttagttt
    65941 tccgcactga cataaactga caaatattta atctcccttt catttggcgt gaaaaattga
    66001 atgagattat tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa
    66061 cttatg
//
LOCUS       DAZGTR010000012        64755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGTR010000012 DAZGTR010000000
VERSION     DAZGTR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.012"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(80..156)
                     /locus_tag="ACHLCY_000889"
     tRNA            complement(80..156)
                     /locus_tag="ACHLCY_000889"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Arg,seq:acg)
     gene            complement(220..296)
                     /locus_tag="ACHLCY_000890"
     tRNA            complement(220..296)
                     /locus_tag="ACHLCY_000890"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(260..262),aa:Arg,seq:acg)
     gene            complement(494..570)
                     /locus_tag="ACHLCY_000891"
     tRNA            complement(494..570)
                     /locus_tag="ACHLCY_000891"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(534..536),aa:Arg,seq:acg)
     gene            complement(574..666)
                     /locus_tag="ACHLCY_000892"
     tRNA            complement(574..666)
                     /locus_tag="ACHLCY_000892"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(630..632),aa:Ser,seq:gct)
     gene            complement(981..1166)
                     /gene="csrA"
                     /locus_tag="ACHLCY_000893"
     CDS             complement(981..1166)
                     /gene="csrA"
                     /locus_tag="ACHLCY_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP0368204.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1401..4031)
                     /gene="alaS"
                     /locus_tag="ACHLCY_000894"
     CDS             complement(1401..4031)
                     /gene="alaS"
                     /locus_tag="ACHLCY_000894"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP0368205.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4159..4659)
                     /gene="recX"
                     /locus_tag="ACHLCY_000895"
     CDS             complement(4159..4659)
                     /gene="recX"
                     /locus_tag="ACHLCY_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP0368206.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4902..5963)
                     /gene="recA"
                     /locus_tag="ACHLCY_000896"
     CDS             complement(4902..5963)
                     /gene="recA"
                     /locus_tag="ACHLCY_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0368207.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6043..6540)
                     /gene="pncC"
                     /locus_tag="ACHLCY_000897"
     CDS             complement(6043..6540)
                     /gene="pncC"
                     /locus_tag="ACHLCY_000897"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP0368208.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6685..7770)
                     /gene="mltB"
                     /locus_tag="ACHLCY_000898"
     CDS             complement(6685..7770)
                     /gene="mltB"
                     /locus_tag="ACHLCY_000898"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP0368209.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8026..8589
                     /gene="srlA"
                     /locus_tag="ACHLCY_000899"
                     /gene_synonym="gutA"
     CDS             8026..8589
                     /gene="srlA"
                     /locus_tag="ACHLCY_000899"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP0368210.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8586..9545
                     /gene="srlE"
                     /locus_tag="ACHLCY_000900"
                     /gene_synonym="gutE"
     CDS             8586..9545
                     /gene="srlE"
                     /locus_tag="ACHLCY_000900"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP0368211.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9556..9927
                     /gene="srlB"
                     /locus_tag="ACHLCY_000901"
                     /gene_synonym="gutB"
     CDS             9556..9927
                     /gene="srlB"
                     /locus_tag="ACHLCY_000901"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP0368212.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9931..10710
                     /gene="srlD"
                     /locus_tag="ACHLCY_000902"
     CDS             9931..10710
                     /gene="srlD"
                     /locus_tag="ACHLCY_000902"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP0368213.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10815..11174
                     /gene="gutM"
                     /locus_tag="ACHLCY_000903"
     CDS             10815..11174
                     /gene="gutM"
                     /locus_tag="ACHLCY_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP0368214.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11241..12014
                     /gene="srlR"
                     /locus_tag="ACHLCY_000904"
                     /gene_synonym="gutR"
     CDS             11241..12014
                     /gene="srlR"
                     /locus_tag="ACHLCY_000904"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP0368215.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12007..12972
                     /gene="gutQ"
                     /locus_tag="ACHLCY_000905"
     CDS             12007..12972
                     /gene="gutQ"
                     /locus_tag="ACHLCY_000905"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP0368216.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12969..14483)
                     /gene="norR"
                     /locus_tag="ACHLCY_000906"
     CDS             complement(12969..14483)
                     /gene="norR"
                     /locus_tag="ACHLCY_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP0368217.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14670..16109
                     /gene="norV"
                     /locus_tag="ACHLCY_000907"
     CDS             14670..16109
                     /gene="norV"
                     /locus_tag="ACHLCY_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP0368218.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCCEHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            16106..17239
                     /gene="norW"
                     /locus_tag="ACHLCY_000908"
     CDS             16106..17239
                     /gene="norW"
                     /locus_tag="ACHLCY_000908"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP0368219.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17465..19717)
                     /gene="hypF"
                     /locus_tag="ACHLCY_000909"
     CDS             complement(17465..19717)
                     /gene="hypF"
                     /locus_tag="ACHLCY_000909"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP0368220.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19870..20397)
                     /gene="hydN"
                     /locus_tag="ACHLCY_000910"
     CDS             complement(19870..20397)
                     /gene="hydN"
                     /locus_tag="ACHLCY_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP0368221.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGVGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20546..21556)
                     /gene="ascG"
                     /locus_tag="ACHLCY_000911"
     CDS             complement(20546..21556)
                     /gene="ascG"
                     /locus_tag="ACHLCY_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP0368222.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALAQHGIALNEKLIA
                     NGRWTPASGAEGVEMLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLICRDSLIAL
                     SR"
     gene            21816..23273
                     /gene="ascF"
                     /locus_tag="ACHLCY_000912"
     CDS             21816..23273
                     /gene="ascF"
                     /locus_tag="ACHLCY_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP0368223.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            23282..24706
                     /gene="ascB"
                     /locus_tag="ACHLCY_000913"
     CDS             23282..24706
                     /gene="ascB"
                     /locus_tag="ACHLCY_000913"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP0368224.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24820..25239)
                     /locus_tag="ACHLCY_000914"
     CDS             complement(24820..25239)
                     /locus_tag="ACHLCY_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0368225.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25236..25547)
                     /locus_tag="ACHLCY_000915"
     CDS             complement(25236..25547)
                     /locus_tag="ACHLCY_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP0368226.1"
                     /translation="MHIISKAPFEESARKYPNDALAHQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25721..26464)
                     /locus_tag="ACHLCY_000916"
     CDS             complement(25721..26464)
                     /locus_tag="ACHLCY_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368227.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKFV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26507..26977)
                     /gene="hycI"
                     /locus_tag="ACHLCY_000917"
     CDS             complement(26507..26977)
                     /gene="hycI"
                     /locus_tag="ACHLCY_000917"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP0368228.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26970..27380)
                     /gene="hycH"
                     /locus_tag="ACHLCY_000918"
     CDS             complement(26970..27380)
                     /gene="hycH"
                     /locus_tag="ACHLCY_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP0368229.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27377..28144)
                     /gene="hycG"
                     /locus_tag="ACHLCY_000919"
     CDS             complement(27377..28144)
                     /gene="hycG"
                     /locus_tag="ACHLCY_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP0368230.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(28144..28686)
                     /gene="hycF"
                     /locus_tag="ACHLCY_000920"
     CDS             complement(28144..28686)
                     /gene="hycF"
                     /locus_tag="ACHLCY_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP0368231.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28696..30405)
                     /gene="hycE"
                     /locus_tag="ACHLCY_000921"
     CDS             complement(28696..30405)
                     /gene="hycE"
                     /locus_tag="ACHLCY_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP0368232.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30423..31346)
                     /gene="hycD"
                     /locus_tag="ACHLCY_000922"
     CDS             complement(30423..31346)
                     /gene="hycD"
                     /locus_tag="ACHLCY_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP0368233.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31349..33175)
                     /gene="hycC"
                     /locus_tag="ACHLCY_000923"
     CDS             complement(31349..33175)
                     /gene="hycC"
                     /locus_tag="ACHLCY_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP0368234.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMRVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(33172..33783)
                     /gene="hycB"
                     /locus_tag="ACHLCY_000924"
     CDS             complement(33172..33783)
                     /gene="hycB"
                     /locus_tag="ACHLCY_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP0368235.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33908..34369)
                     /gene="hycA"
                     /locus_tag="ACHLCY_000925"
     CDS             complement(33908..34369)
                     /gene="hycA"
                     /locus_tag="ACHLCY_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP0368236.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34581..34931
                     /gene="hypA"
                     /locus_tag="ACHLCY_000926"
     CDS             34581..34931
                     /gene="hypA"
                     /locus_tag="ACHLCY_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0368237.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34935..35807
                     /gene="hypB"
                     /locus_tag="ACHLCY_000927"
     CDS             34935..35807
                     /gene="hypB"
                     /locus_tag="ACHLCY_000927"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP0368238.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35798..36070
                     /gene="hypC"
                     /locus_tag="ACHLCY_000928"
     CDS             35798..36070
                     /gene="hypC"
                     /locus_tag="ACHLCY_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP0368239.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            36070..37191
                     /gene="hypD"
                     /locus_tag="ACHLCY_000929"
     CDS             36070..37191
                     /gene="hypD"
                     /locus_tag="ACHLCY_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP0368240.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            37188..38198
                     /gene="hypE"
                     /locus_tag="ACHLCY_000930"
     CDS             37188..38198
                     /gene="hypE"
                     /locus_tag="ACHLCY_000930"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP0368241.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38272..40350
                     /gene="flhA"
                     /locus_tag="ACHLCY_000931"
     CDS             38272..40350
                     /gene="flhA"
                     /locus_tag="ACHLCY_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP0368242.1"
                     /translation="MSYTPMSDLGQQRLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAKCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(40387..40740)
                     /gene="ygbA"
                     /locus_tag="ACHLCY_000932"
     CDS             complement(40387..40740)
                     /gene="ygbA"
                     /locus_tag="ACHLCY_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP0368243.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40817..40951)
                     /locus_tag="ACHLCY_000933"
     CDS             complement(40817..40951)
                     /locus_tag="ACHLCY_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368244.1"
                     /translation="MIPELIISGEEANCEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            41027..43588
                     /gene="mutS"
                     /locus_tag="ACHLCY_000934"
     CDS             41027..43588
                     /gene="mutS"
                     /locus_tag="ACHLCY_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP0368245.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43694..44350
                     /gene="pphB"
                     /locus_tag="ACHLCY_000935"
     CDS             43694..44350
                     /gene="pphB"
                     /locus_tag="ACHLCY_000935"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0368246.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(44401..45168)
                     /gene="ygbI"
                     /locus_tag="ACHLCY_000936"
     CDS             complement(44401..45168)
                     /gene="ygbI"
                     /locus_tag="ACHLCY_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP0368247.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            45364..46272
                     /gene="ltnD"
                     /locus_tag="ACHLCY_000937"
     CDS             45364..46272
                     /gene="ltnD"
                     /locus_tag="ACHLCY_000937"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP0368248.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            46269..47531
                     /gene="otnK"
                     /locus_tag="ACHLCY_000938"
     CDS             46269..47531
                     /gene="otnK"
                     /locus_tag="ACHLCY_000938"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP0368249.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDLARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAESVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            47528..48166
                     /gene="ygbL"
                     /locus_tag="ACHLCY_000939"
     CDS             47528..48166
                     /gene="ygbL"
                     /locus_tag="ACHLCY_000939"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0368250.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            48171..48947
                     /gene="ygbM"
                     /locus_tag="ACHLCY_000940"
     CDS             48171..48947
                     /gene="ygbM"
                     /locus_tag="ACHLCY_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP0368251.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            49036..50400
                     /gene="ygbN"
                     /locus_tag="ACHLCY_000941"
     CDS             49036..50400
                     /gene="ygbN"
                     /locus_tag="ACHLCY_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP0368252.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(50439..50675)
                     /locus_tag="ACHLCY_000942"
     CDS             complement(50439..50675)
                     /locus_tag="ACHLCY_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP0368253.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(50686..52107)
                     /locus_tag="ACHLCY_000943"
     CDS             complement(50686..52107)
                     /locus_tag="ACHLCY_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP0368254.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGGFYLDKACVVSRDPLDPDNFGKQNV
                     GIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATPLK
                     YDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFTGH
                     YSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFPEV
                     QAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFNLP
                     QVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHYSQ
                     PVVDLPETKAWAEKLTAMLAARK"
     gene            complement(52107..52700)
                     /locus_tag="ACHLCY_000944"
     CDS             complement(52107..52700)
                     /locus_tag="ACHLCY_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP0368255.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            52847..53254
                     /locus_tag="ACHLCY_000945"
     CDS             52847..53254
                     /locus_tag="ACHLCY_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0368256.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(53375..54367)
                     /gene="rpoS"
                     /locus_tag="ACHLCY_000946"
     CDS             complement(53375..54367)
                     /gene="rpoS"
                     /locus_tag="ACHLCY_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP0368257.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(54430..55524)
                     /gene="nlpD"
                     /locus_tag="ACHLCY_000947"
     CDS             complement(54430..55524)
                     /gene="nlpD"
                     /locus_tag="ACHLCY_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP0368258.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITRADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(55709..56335)
                     /gene="pcm"
                     /locus_tag="ACHLCY_000948"
     CDS             complement(55709..56335)
                     /gene="pcm"
                     /locus_tag="ACHLCY_000948"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP0368259.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(56329..57090)
                     /gene="surE"
                     /locus_tag="ACHLCY_000949"
     CDS             complement(56329..57090)
                     /gene="surE"
                     /locus_tag="ACHLCY_000949"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP0368260.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(57071..58120)
                     /gene="truD"
                     /locus_tag="ACHLCY_000950"
     CDS             complement(57071..58120)
                     /gene="truD"
                     /locus_tag="ACHLCY_000950"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP0368261.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEYI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLNDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(58117..58596)
                     /gene="ispF"
                     /locus_tag="ACHLCY_000951"
     CDS             complement(58117..58596)
                     /gene="ispF"
                     /locus_tag="ACHLCY_000951"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP0368262.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(58596..59306)
                     /gene="ispD"
                     /locus_tag="ACHLCY_000952"
     CDS             complement(58596..59306)
                     /gene="ispD"
                     /locus_tag="ACHLCY_000952"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP0368263.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(59325..59636)
                     /gene="ftsB"
                     /locus_tag="ACHLCY_000953"
     CDS             complement(59325..59636)
                     /gene="ftsB"
                     /locus_tag="ACHLCY_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP0368264.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(59830..60153)
                     /gene="ygbE"
                     /locus_tag="ACHLCY_000954"
     CDS             complement(59830..60153)
                     /gene="ygbE"
                     /locus_tag="ACHLCY_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP0368265.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(60203..60808)
                     /gene="cysC"
                     /locus_tag="ACHLCY_000955"
     CDS             complement(60203..60808)
                     /gene="cysC"
                     /locus_tag="ACHLCY_000955"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP0368266.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(60808..62235)
                     /gene="cysN"
                     /locus_tag="ACHLCY_000956"
     CDS             complement(60808..62235)
                     /gene="cysN"
                     /locus_tag="ACHLCY_000956"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP0368267.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTSGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(62237..63145)
                     /gene="cysD"
                     /locus_tag="ACHLCY_000957"
     CDS             complement(62237..63145)
                     /gene="cysD"
                     /locus_tag="ACHLCY_000957"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP0368268.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            63397..64434
                     /gene="iap"
                     /locus_tag="ACHLCY_000958"
     CDS             63397..64434
                     /gene="iap"
                     /locus_tag="ACHLCY_000958"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP0368269.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT        15225 a        17697 c        16774 g        15059 t
ORIGIN      
        1 gcaacgttat catccctggt gcctgcggtt aattccgcta cctcttcgat accttcattg
       61 ctgaagattt cggagaatat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
      121 gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaaattta ttttactgct
      181 gatattgata ccgctactaa agccatatca agtaagagat ggtgcatccg ggaggattcg
      241 aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcatcg
      301 ggaaacttac tttactgcag attttttgat accgctacta aagccgtatc aagtaagaga
      361 tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt gctaaagcaa
      421 cgttatcctc cctggtgcct gcggttatac cgccacctct tccatacctt catcgctgaa
      481 agtacgtaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc
      541 gagtactcta tccagctgag ctacggatgc aaatggcggt gaggcgggga ttcgaacccc
      601 ggatgcagct tttgaccgca tactccctta gcaggggagc gccttcagcc tctcggccac
      661 ctcaccacac gcctcttacg agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg
      721 tggcgcacat attactttct gagacttata agtcaaacaa tttttcctac acttttatcg
      781 tttgcacact tcacgttcaa ttagtctgta aaaacgtcaa aaagagtgtt ttatcaacag
      841 aaaaatggag gtctgacaga tagtagtaat gcaaaaaatg gagacttaag ttgaatgaac
      901 gggagtaaag cgaaaagact atagagtgaa ggagaaattt tgagggtgcg tctcaccgat
      961 aaagatgaga cgcggaaaga ttagtaactg gactgctggg atttttcagc ctggatacgc
     1021 tggtagatct cttcacggtg aacagaaact tccttcgggg catttacgcc aatacgtacc
     1081 tggttgccct ttacccctaa aactgtcacg gtgacctcat ccccaatcat gagggtctca
     1141 ccaactcgac gagtcagaat cagcattctt tgctccttga aagattaaaa gagtcgggtc
     1201 tctctgtatc ccggcattat ccatcatata acgccaaaaa gtaagcaatg acaaacacat
     1261 tacatgtaag cagtcatggc attacattct gttaaaccta agtttagccg atatacacaa
     1321 cttcaacctg actttatcgt tgtcgatagc gttaatgcga atgccgtgaa gcgagtccac
     1381 ggcattgcct gacgcttata ttattgcaat ttcgcgctga cccagccttt cacactggct
     1441 aacgctgcag gtaaggccgc agcatccgta ccaccggctt gcgccatgtc aggacgtcca
     1501 ccacccttgc cgcccacctg ctgagcgacc ataccaatca gttcccctgc tttcacacga
     1561 tctgtgacgt ccttagatac gcctgcaatc agagaaacct taccttcggc taccgttgcc
     1621 agcacgataa ttgtcgaccc cagctgattt tttaaatcgt caaccatggt acgcaacatt
     1681 ttcggctcaa caccgctaag ctcgctaacc aacagcttaa caccattaac atcaattgcc
     1741 ttactggaaa gatttgcgct ctcctgtgcg gcagcttgtt ctttaagctg ttgtagttct
     1801 ttttctaact gacgcgtacg ttccagtact gagcgcactt tatccgccag attattgcta
     1861 tcgcctttca gcagatgcgc gacttcgctt aagcgatcgc tgtctgcatg aacggtggtg
     1921 atagcgcctt ctccggttac cgcttcgata cgacgaacgc ctgcagcagt acccgattca
     1981 gagatgatgc ggaacagacc aatatcacca gtgcggctgg cgtgagtacc gccacacagc
     2041 tcggtggaga aatcgcccat gctcagcacg cgtacacgct catcatactt ctcgccgaac
     2101 agcgccatcg cacctttcgc tttcgccgct tcgagatcca tgatgttggt ttcgatcggc
     2161 aaattgcgac gaatctgtgc gttcaccagg tcttcgaccg cacgaatctc ttccggtttc
     2221 atcgcttcgt tgtgtgagaa gtcgaagcgc agcaccttgt cgttaaccag tgaacctttc
     2281 tgcgatacat gagtccccag aacctggcgc agcgcagcgt gcatcaggtg cgttgcggag
     2341 tgattcagac gaatacgggc gcgacgagcc tcatcaacat cagcctgcac agcgtcgccc
     2401 actttcagag aacccgcagc aagtttaccg atgtgaccaa tcgcctggcc gtatttctgc
     2461 gtatcttcca ccgcgaagga gaagttagcg cctttcagtt cgcctttatc accaacctga
     2521 ccgccggatt ccgcatagaa tggtgtttga tccagcacga ccacagcttc ctggcctgca
     2581 ttgattgcat caaccgcttt accatcaaca aacagcgcgg tcactttgcc gttcagttcc
     2641 agatggtcat agcctttaaa ttcagatgca ctatcaacac ggatcattgc gttgtaatcg
     2701 gcaccaaagc cgctggcttc gcgcgcgcga cgacgctgct cttccattgc tgcttcaaaa
     2761 ccagcttcgt caactttgat gttgcgctca cgacaaacat cagccgtcag gtcaaccggg
     2821 aagccatagg tgtcgtacag acggaaagcc gtttcaccat ccagcgtatc accagaaagt
     2881 tttgccagct cttcatccag caacgccaga ccgcgctcca gagtacgggc aaactgctct
     2941 tcttcagtct tcagcacctg ctcaacctgc gcctgctggc gtttcaggtc ttcacccgca
     3001 gagcccataa cgtcgatcag cggaccaacc agtttgtaga agaaggtttc tttcgcgccg
     3061 agcatattgc cgtgacgcac tgcgcgacga atgatacgac gcagtacata accacggttt
     3121 tcattggacg gcattacgcc atccgcgatc aggaacgcac aagaacgaat gtggtcagcg
     3181 attacgcgca gcgatttatt gctcagatcg gtcgcgccag tgactttcgc taccgcctgg
     3241 atcaacgtgc ggaacaggtc gatgtcatag ttagagttaa cgtgttgcag caccgcagca
     3301 atacgctcca gacccatacc ggtatctaca gacggcttcg gcagcggttc catcgtgcca
     3361 tctgcctggc ggttgaactg catgaagacg atgttccaga tctcaatgta gcggtcgccg
     3421 tcttcttccg gacttcccgg aggaccaccc caaatgtggt cgccgtgatc gtagaagatt
     3481 tcggtgcacg ggccgcacgg accagtgtca cccatctgcc agaagttgtc agatgcgtat
     3541 ggcgcacctt tgttatcgcc gatgcgaata atacgttcgc gcgggatccc gacttctttt
     3601 tcccagattt cgtaggcttc gtcgtcgctt tcatagacgg taacccacag acgctctttc
     3661 ggcagggcaa accatttttc gctggtcagc agttcccatg caaactgaat ggcatcgtgt
     3721 ttgaaatagt cgccgaagct gaagttgccc agcatttcga agaaggtatg gtgacgcgcg
     3781 gtgtaaccga cgttttccag gtcgttgtgt ttaccacccg cacgcacgca gcgttgggaa
     3841 gtggtagcgc gggaataatt acgcttgtcg agcccaagga acacatcctt gaactggttc
     3901 atcccggcgt tggtaaacaa caaagttggg tcgttatggg gtaccaggga gctgctggca
     3961 actacctgat gtcccttact atggaaaaag tcgagaaacg cctgacggat ctcagcggtg
     4021 ctcttgctca taattatcct gaaatcaagc taacgaaata tcgccaccag ctccagcgtg
     4081 tcttaaccgc cgggctggta actgaaaagt gggaataaga taagttttct tgactgggaa
     4141 gtaaaatacc gtatgcgttc agtcggcaaa atttcgccaa atatcctgga tatcttccat
     4201 cagatagcca cgatagagca gaaaacgctg gatcttaact ttttctgaaa agacagttgg
     4261 caaaggttca ccatattttc gcgtcgcctg atcgcgcgcc agtgcgcacc agtcgatgtc
     4321 acattcacgc atcgcttttt ctgtcgcttc gcgggaaata cctttctgat tcagttcctg
     4381 acgaatacgt gcaggtccat aacctttgcg gctacggctg gcgataaagc gcgcaacaaa
     4441 tcggctgtca tcgagatagc catgttcatg gcaccaggca ataacgcgct cgtaatcttc
     4501 tgccgtagca tcaatctctt ctgggccatt tttgcccata atcggtgcca cgagtttacg
     4561 tcgcagttct tgctcactgt gatcgcgcac cgccagaatg cgtaccgcac gatccaacag
     4621 gcgagcatat gccgggcgac gggacgttga ttctgtcatg gcatatcctt acaacttaaa
     4681 aaagcaaaag ggccgcagat gcgacccagg ttttgctgat aaacagattt aatagtcaat
     4741 gagtgtgcag aatgttcccg caggcccaat tcgttagagg atcgaggtga gagtaatttt
     4801 tcacgccagt gctggcctgt tcccctcacc ctaaccctct ccccaaaggg gcgaggggac
     4861 cgtccgtgca cacattatct attatcatca ataacgaacg attaaaaatc ttcgttagtt
     4921 tctgctacgc cttcgctgtc atctacagag aaatccggcg ttgagttcgg gttgctcagc
     4981 agcaactcac gtactttctt ctcgatctct ttcgcggttt ccgggttatc tttcagccag
     5041 gcagttgcat tcgctttacc ctgaccgatc ttctcacctt tgtagctgta ccacgcgcct
     5101 gctttctcga tcagcttctc ttttacgccc aggtcaacca gttcgccgta gaagttgata
     5161 ccttcgccgt agaggatctg aaattcagcc tgtttaaacg gtgcagcgat tttgttcttc
     5221 accactttca cgcgggtttc gctacccacc acgttttcgc cctctttcac cgcgccgata
     5281 cgacggatgt cgagacgaac agaggcgtag aatttcagcg cgttaccacc ggtagtagtt
     5341 tccgggttac cgaacatcac accaattttc atacggatct ggttgatgaa gatcagcagc
     5401 gtgttggact gcttcaggtt acccgccagc ttacgcatcg cctggctcat catacgtgcc
     5461 gcaaggccca tgtgagagtc gccgatttcg ccttcgattt ccgctttcgg cgtcagtgcc
     5521 gccacggagt caacgacgat aacgtctact gcgccagaac gcgccagggc gtcacagatt
     5581 tccagtgcct gctcgccggt gtccggctgg gagcacagca ggttatcgat atcgacgccc
     5641 agtttacgtg cgtagattgg gtccagcgcg tgttcagcat cgataaacgc acaggtttta
     5701 ccttcacgct gcgctgcggc gatcacctgc agcgtcagcg tggttttacc ggaagattcc
     5761 ggtccgtaga tttcgacgat acggcccatc ggcagaccac ctgccccaag cgcgatatcc
     5821 agtgaaagcg aaccggtaga gatggtttcc acatccatgg aacggtcttc acccaggcgc
     5881 atgatggagc ctttaccaaa ttgtttctca atctggccca gtgctgccgc caacgctttc
     5941 tgtttgtttt cgtcgatagc catttttact cctgtcatgc cgggtaatac cggatagtca
     6001 atatgttctg ttgaagcaat tatactgtat gctcatacag tatcaagtgt tttgtagaaa
     6061 ttgttgccac aaggtctgca atgcatacgc agtagcctga cggcgcaccg catcacggtc
     6121 accgctgaag cattcccgcc gggtaatgcc ttcaccgcgg gcagtggcaa aagcaaacca
     6181 gacggtgccg acaggcttct cttcactgcc gccatccggc ccggcgatac cactaataga
     6241 cacggcataa tcagcacgag ccgctttcag tgcacctatc gccatttcca ccacgacggg
     6301 ttcactcacc gcgccatgct gcgccagcgt ctcttcgcgt acgccgatca tctgcgcttt
     6361 ggcttcgtta ctgtaggtga caaatccgcg ttcaaaccag gcggagctac cggcaatatc
     6421 ggtaatcact ttcgctaccc aaccaccggt acaagactcg gcagttgtta cggttgcgcc
     6481 acgggctttc agcgcctgcc caacctgttc acttaactgc atcagttcac tgtcagtcat
     6541 cacaacctct gtcagtcaaa aatttatgcc ggacaagata gcactttcgt caggaagatg
     6601 gtgttggcgt tgcaatttta cgaggaggat tcagaaaaaa gctgattagc cagagggaag
     6661 ctcacgcccc cctcttgcaa atagttactg cacgcgcgcc agcgccacgg cttgtcctaa
     6721 ctgccagacc gccattgcgt aatgggtgct gtggttataa cgggtaatgg tgtagaagtt
     6781 cggcagaccg taccagtact ggtaaccggt accgacatcc agacgcagca ggctggcttg
     6841 ttgatggttg cccagcggct gctgtggcgt taaacctgcc gtggcaagct gcgaaatgct
     6901 gtacctggtt ttgaagccat ttggcaagcc tggagcctga ccgtttgcca ttaccgcgac
     6961 ctgatcgcct ttcacccagc cgtgcgcttt gaaatagttc gccacgctac cgatcgcatc
     7021 aaccggatcc cacaggttga tatgcccgtc gccgctgaaa tctaccgcat attgtttgta
     7081 agacgacggc ataaactgtc cgtagcccat cgccccggca aaggaacctt tcagattgag
     7141 cggatcgtcc tgctcgtcgc gcgccatcaa caggaaggtt tccagctcgc cagagaaata
     7201 ctccgcgcgg cgtgggtagt taaatgacag cgttgccagc gcatcgagga tgcgagtttt
     7261 ccccatcacg cgcccccagc gggtttcaac gccgataatc ccgacgataa tttccggcgg
     7321 tacgccatac acctgccacg cgcgattcaa cgcatcttca tactgattcc agaaaaccac
     7381 accgttctgc acgttgtccg gcgtaataaa ttttttgcga taacggagcc atgcgccgtt
     7441 cggacctgat ggcggtttca ccgatgtggt tggtgcctgg ttatccatca gccgcagtac
     7501 cgaatccaga cgcttcgcct gggagagaat ttcctgcaac tgctgacgat cgaaaccgtg
     7561 tttgttcacc attttgtcga tgaactgctg ggcattcggg ttattagcga aatcgccgcc
     7621 catctgcatc acattgtgct gcggctcaag caggaagccg ccagacggcg ttccggtggt
     7681 cgtttcagtc tcagtaggtt ttggcttgct gctacaggcg gcaagcaaca caaaaagggg
     7741 aagcaatgtt acataacgac gcttgaacat gaggggtcca tttaacagat tcaaccaggg
     7801 gcaagtatgg taaagcatca cgccccacac aaggaagcgg tagtcactgc ccgatacgga
     7861 ctttacataa ctcaactcat tcccctcgct atccttttac tcaaactttc aaattaaaat
     7921 atttatcttt cattttgaga tcaaaataac acttttaaat ctttcaatct gattagatta
     7981 ggttgccgtt tggtaataaa acaataaatc ctgaaggaga gaacaatgat agaaaccatt
     8041 actcatggtg cagagtggtt tatcgggctg ttccaaaagg gcggagaggt gtttaccggg
     8101 atggtgaccg gcattcttcc gctgttgatt agcctgctgg ttatcatgaa cgcactgatt
     8161 aattttatcg gtcagcatcg tattgaacgt tttgctcaac gttgcgccgg taaccctgtt
     8221 tcccgttacc tactgttacc gtgcattggc acgtttgtct tttgcaatcc gatgacctta
     8281 agcctgggtc gctttatgcc ggaaaagtac aaacccagct actacgcggc ggcctcttat
     8341 agctgccact caatgaatgg cctcttcccc catatcaacc ctggcgaact gtttgtttat
     8401 cttggcattg ccagcggtct gacaacgctg aacctgccac ttggcccact ggcggtgagt
     8461 tatctgctgg ttggtctggt caccaatttc ttccgcggct gggtgaccga tctgaccacc
     8521 gccatttttg agaaaaagat gggcattcaa cttgaacaaa aagttcacct ggcaggagca
     8581 acatcatgac gcatattcgg atcgaaaaag gaacgggtgg ctggggcggc ccgcttgagc
     8641 tgaaagccac gccgggaaaa aaaatcgtct atatcaccgc cggtacccgg cctgcgattg
     8701 ttgacaaact ggcacagctt actggctggc aggctattga cggatttaaa gaaggtgaac
     8761 ccgcggaggc ggaaattggt gtcgcggtaa tcgactgtgg cggcacatta cgctgcggca
     8821 tctatccgaa acgacgtatt cccaccatta atatccactc gacgggcaag tccggtccgc
     8881 tggcgcagta cattgtggaa gatatttatg tctctggcgt aaaagaagaa aacatcactg
     8941 tagtgggtga tgcgacacca caaccctctt ccgtgggccg tgactatgac accagtaaga
     9001 aaatcaccga acaaagcgat ggtttactgg cgaaggtggg aatgggcatg gggtccgccg
     9061 ttgcggtgct gtttcaatct ggtcgtgaca ccatcgacac tgtattaaaa accattctgc
     9121 cgtttatggc attcgtctcg gcgctcattg gcatcattat ggcttctggc cttggtgact
     9181 ggattgccca cggtcttgct ccgctggcga gccatccact gggtctggtc atgctggcgc
     9241 tcatctgctc cttcccactg ctttcacctt tcctcggccc aggcgcagtt atcgcacagg
     9301 ttatcggcgt attgattggc gtgcagattg gtctcggcaa tattccgccg catctggctt
     9361 taccggcact gtttgccatc aacgcgcagg cggcctgcga cttcatcccg gtcggtttgt
     9421 cgctggcgga agcccgtcag gacacggttc gcgtcggtgt cccttctgta ctggtgagcc
     9481 gctttttaac cggcgcaccg actgtactga tcgcctggtt tgtctccggt tttatctatc
     9541 aatagaggct gaaacatgac cgttatttat cagaccacca tcacccgtat cggcgcgagt
     9601 gccactgacg ccctcagcga ccagatgctc atcacctttc gtgaaggcgc gccagcggac
     9661 ctcgaagagt attgcttcat tcattgccac ggcgagttga aaggtgcact ccatcccggt
     9721 ttgcaattta cgctcgggca gcatcgctat ccggtgaccg ctgttggcag cgtggcggaa
     9781 gacaaccttc gcgaactggg tcatgtcacc ctgcgcttcg atggtttaaa cgaagcggaa
     9841 tttccgggca ctgtccatgt ggcaggccct gtacccgacg atatcgcgcc gggatcggtt
     9901 ttgaagtttg aatctgttaa ggagtaaaaa atgaatcagg ttgccgttgt catcggtggt
     9961 gggcaaacct taggcgcgtt cctgtgccac ggtctggctg ccgaggggta tcgcgtcgcg
    10021 gttgtcgata ttcagagcga caaagccgca aatgtggcac aagaaattaa cgccgaatat
    10081 ggtgaaagta tggcgtacgg ttttggtgct gacgccacta gcgagcaaag cgttctggcg
    10141 ctctctcgtg gggtagatga aatctttggt cgcgtggatt tgctggtcta tagcgccgga
    10201 atagcaaaag cagcctttat cagcgacttc cagctcggcg attttgaccg ttcgctacaa
    10261 gtgaatctgg tgggttattt cctgtgtgcc cgtgaatttt cgcgtttgat gatccgcgac
    10321 gggattcagg gtcgcattat tcagatcaac tcgaaatccg gcaaagtggg cagcaaacac
    10381 aactctggct acagcgcagc aaaatttggt ggcgtcgggc tgactcaatc actggcgctg
    10441 gatctggcgg agtacggcat tacggtgcat tcactgatgc tcggtaacct gctgaaatcg
    10501 ccgatgttcc agtcactgtt gccacaatac gcgaccaagc tgggtatcaa accggatcaa
    10561 gtcgagcagt attacatcga caaagtaccg ctcaaacgcg gctgcgatta tcaagatgtg
    10621 ctgaatatgc tgctgttcta cgccagtcct aaggcgtcgt actgcaccgg acagtcgatc
    10681 aatgtcaccg gcggtcaggt gatgttctga tcaacagcgg agatccatta aggatctccg
    10741 tgagactata gaatgcctga tgcgctacgc tcatcaggca tacaggactt ccgccactac
    10801 attaaggaaa agttatggta tccgcactca tcaccgtcgc cgttatcgcc tggtgtgcgc
    10861 aactggcctt aggcggctgg caaatttctc gttttaaccg tgccttcgac acactatgcc
    10921 agcaagggcg ggttggcgtg ggccgttcca gcgggcgctt taaaccgcgg gtcgtggtcg
    10981 ccatcgcgct ggacgatcag cagcgcatcg tcgacacctt gtttatgaaa ggactgaccg
    11041 tcttcgcccg accgcaaaaa attcccgcaa ttaccggtat gcatgcgggt gatttacagc
    11101 ccgatgtgat ctttccccat gatccactat cacagaatgc tctatcattg gcgcttaaac
    11161 tgaaacgtgg ataatttcgt tgtgaatgtt acttgcttgc gaagttatca ttttgaaacc
    11221 taaatcaggt aatcacgccc atgaaacctc gtcagcgtca ggccgccatt ctggagtatc
    11281 tgcaaaagca gggtaaatgc tcggttgaag aattggcgca atactttgac accacaggca
    11341 caaccattcg caaagatctg gtcattctgg aacatgccgg aaccgtcatt cgtacttatg
    11401 gcggagtggt gttgaataaa gaggaatccg atccgcctat cgatcataaa acactcatca
    11461 acacccacaa gaaagagctg attgcagaag ctgccgttag ttttatccat gatggcgatt
    11521 cgatcattct tgatgctggc agtaccgttt tgcagatggt tcccctgctc tcgcgcttta
    11581 ataacatcac ggtgatgacc aacagcctgc atatcgtcaa tgcgctatcc gaactggata
    11641 acgaacaaac tatcctgatg ccaggcggaa cgtttcgtaa aaaatcggcc tcatttcacg
    11701 gacagctggc agagaatgcc ttcgaacatt tcagtttcga taaattgttt atgggcaccg
    11761 acggcatcga tctcaatgcg ggcgtaacca ccttcaacga ggtttatacc gtcagtaagg
    11821 caatgtgcaa tgccgcgcgc gaagtgattt tgatggcgga ctcatcaaag tttggccgta
    11881 aaagccccaa cgtagtttgc agtcttgaaa gcgtcgataa gctgattacc gacgcaggta
    11941 tcgatccggc gtttcgtcag gcgctggaag agaaagggat cgatgtgatc ataaccggag
    12001 agagcaatga gtgaagcact actgaacgcg ggacgtcaga cgttaatgct ggagttgcag
    12061 gaagcaagcc gtttaccgga acgtctgggc gatgattttg ttcgcgccgc caatatcatc
    12121 ctgcactgcg aaggcaaagt ggtggtttcg ggaattggca aatcgggcca cattggtaag
    12181 aaaatcgccg caacacttgc cagtaccggc acaccggctt tttttgtcca tccggcagaa
    12241 gcactgcacg gcgatctggg gatgattgaa agccgcgatg tgatgctgtt tatctcttac
    12301 tccggtggcg cgaaggaact ggatctgatt attccgcgtc tggaagataa atctatcgcg
    12361 ctgctggcga tgaccggcaa accgacgtca ccgctgggcc tggcggcaaa agcggtgctg
    12421 gatatctccg tagaacgcga agcctgcccg atgcaccttg cgccgacctc cagcaccgtc
    12481 aataccctga tgatgggtga cgcgctggcg atggcggtca tgcaggcgcg cggatttaat
    12541 gaagaagatt ttgcccgctc ccacccagcc ggggcactgg gcgctcgctt gctgaataaa
    12601 gtgcatcatc tgatgcgccg tgacgatgcc atcccacagg tggcgttaac cgccagcgtg
    12661 atggatgcga tgctggaact cagccgcacc ggtctggggc tggtggcggt atgtgacgct
    12721 caacaacagg tacaaggcgt ctttaccgac ggcgatttac gtcgctggct ggttggcggc
    12781 ggcgcactca ccacgccagt taatgaagcg atgacgacag gcggcaccac attacaggca
    12841 caaagtcgcg ccatcgacgc caaagagatc ctgatgaagc gcaaaatcac tgccgcaccg
    12901 gtggtggatg aaaacggcaa actcaccggc gcaattaacc tgcaggattt ctatcaggcc
    12961 gggattattt aatccttcaa tcccagacgt ttcgccagcc gatgcaggtt ggcgacgtcg
    13021 gtttccagca tccgcgcgca ggcagcccag ttgtgatgat tttgtgccag tgcctggcga
    13081 atggtttcac gctggaacgc ttctgtcgct tcacgcaggt tttgcttaac aacgggcacc
    13141 gccgccgctt ctggcggcgg caacgtcacc tcaggaaaag caaaatgttg tgcctcaaga
    13201 atcacttcat cgccgctgcg agtggctctc gccagcacta ccgcccgatg aatagcatgt
    13261 tccagttcgc gcacgttccc cggaaaacga tagtgttgca gtaaatttcg cgctccggca
    13321 cttaatacca cgcgggagag ccccagccgc aaacgacact gctcgcagaa ataccccgcc
    13381 agcagaatga catcatcgcc ccgctcacgc agcggcggca ccgaaagtgg aaacacgctc
    13441 aggcgatgaa acaaatcggc gcggaatcgc cctgccagca cctcttcgcg taagtcgcgg
    13501 ttagtcgccg ccagcacgcg cacatcgacc cgcaaactac ggtcatcgcc aacgcgctga
    13561 atatcgccat actgcaacac cctcagcagc ttggcctgca atgccaacga caactcgccg
    13621 atctcatcga gaaacagagt gccgttatcc gccatttcaa acttcccgct gcggttactg
    13681 atagcgccag taaacgctcc tttcacatgc ccgaacaact cactttccgc cacactttcc
    13741 ggcagtgcgg cacagttgag atagaccagc ggattcaccg cccgtggcga ggcttcatga
    13801 atcgctttcg ccaccagctc cttaccggtt cccgtctcac cgctgatcag aacgttgaga
    13861 tcggacgccg ccacaatctc aatctctttt ttcaattgcg tcatgcctgg cgacaagcca
    13921 atcatctgcg tctgtttcac cgcttcaaac ggcgcggcat cgcccggcag catattctgg
    13981 ctttccagtt gttcaatcag caacgcattg cttaacgctc ccgccgccag cgcggcaatc
    14041 agccgtaact cttcgtcgct gaaaacatcg aactgatcgg gctgcatccc gtcgagcgtc
    14101 agtgcgccga tcaggttttg cccggcaaac aatggcagac caacgcaggc gtgaaccttc
    14161 agactctcct gcccaggaat caaaccgtca tagggatcgg gcaattcgct gtctgcagga
    14221 aagcgcacca cgtccccggc gcgggcaatc gcttcaagcc gtggatgccc ttccagcgca
    14281 aagcgtctac cgagtacatc ctttgccaga ccgtcgatgg caagcggaat aaactgccgc
    14341 gaatcgtaac gtagcaacgc agacgcatcg cactccagca cctgacgtag cgtggtgatc
    14401 aggcgctgaa aacgatcctg atgaccaatc ccacgctgca attcgatggc gatattcgcc
    14461 agcacatcaa cggaaaaact catctttgcc tcactgtcaa tttgactata gatattgtca
    14521 tatcgaccat ttgattgata gtcattttga ctactcatta atgagcataa ttttatttat
    14581 agagtaaaaa caatcagata aaaaactggc acgcaatctg caattagcaa gacatctttt
    14641 tagaacacgc tgaataaatt gaggttgcta tgtctattgt ggtgaaaaat aacattcatt
    14701 gggttggtca acgtgactgg gaagtgcgtg attttcacgg cacggaatat aaaacgctgc
    14761 gcggcagcag ttacaacagc tacctcatcc gcgaagaaaa aaacgtgctg atcgacaccg
    14821 tcgaccataa attcagccgc gaatttgtgc agaacctgcg taatgaaatc gatctggcgg
    14881 atatcgatta catcgtgatt aaccatgcag aagaggacca cgctggggcg ctgaccgaac
    14941 tgatggcgca aattcccgat acgccgatct actgtactgc caacgctatc gactcgataa
    15001 atggtcatca ccatcatccg gagtggaatt ttaatgtggt gaaaactggc gacacgctgg
    15061 atatcggcaa cggcaaacag ctcatttttg tcgaaacacc aatgctgcac tggccggaca
    15121 gcatgatgac ttacctgaca ggcgacgcgg tgctgttcag taacgatgct ttcggtcagc
    15181 actactgcga cgagcatctg ttcaacgatg aagtggatca gacggagctt ttcgagcagt
    15241 gccagcgtta ctacgccaat atcctgacgc cgttcagccg cctggtaacg ccgaaaatta
    15301 ccgagatcct gggctttaac ttaccagtcg atatgatagc gacctcccac ggcgtggtct
    15361 ggcgcgataa cccgacgcaa attgtcgagc tgtacctgaa gtgggccgct gattatcagg
    15421 aagacagaat caccattttc tacgacacca tgtcgaataa cacccgcatg atggctgacg
    15481 ctatcgccca gggaattgcg gaaaccgacc cacgcgtggc ggtgaaaatt ttcaacgtcg
    15541 cccgaagcga taaaaacgaa atcctgacca atgtcttccg ctcaaaaggc gtgctggtcg
    15601 gcacttcgac gatgaataac gtgatgatgc cgaaaatcgc cgggctggtg gaggagatga
    15661 ctggtttacg cttccgtaac aaacgcgcca gtgctttcgg ctctcacggc tggagcggcg
    15721 gtgcggtgga tcgtctttcc acgcgcctgc aggatgcggg tttcgaaatg tcgcttagcc
    15781 tgaaagccaa atggcgacca gaccaggacg ctctggagtt atgttgtgaa cacggtcgcg
    15841 aaatcgcccg tcagtgggcg ctcgcgccgc tgccgcagag cacggtgaat acggtggtta
    15901 aagaagaaac ctctgccacc acgacggctg acctcggccc acggatgcag tgcagcgtct
    15961 gccagtggat ttacgatccg gcaaaaggcg agccaatgca ggacgttgcg ccaggaacgc
    16021 cgtggagtga agttccggat aacttcctct gcccggaatg ctccctcggc aaagacgtct
    16081 ttgaagaact ggcatcggag gcaaaatgag taacggcatt gtgatcattg gttcgggctt
    16141 cgccgcccgc caactggtga aaaatattcg caaacaggac gccactattc cattaaccct
    16201 gattgccgcc gacagcatgg atgagtacaa caaacctgac ctcagccatg ttatcagtca
    16261 ggggcaacgt gccgacgacc ttacccgcca gacggcgggt gaatttgccg agcagtttaa
    16321 tctgcgcctg tttccgcaca cctgggtgac ggatatcgat gccgaagccc gtgtggtgaa
    16381 aagccagaat aatcagtggc aatacgacaa gctagtgctg gcaaccggtg ccagcgcctt
    16441 tgtcccgcca gtgcctgggc gtgagttaat gctgacgtta aatagtcagc aagagtatcg
    16501 cgcctgtgaa acgcaactgc gggatgcccg acgcgtgttg attgtcggtg gtggcttgat
    16561 tggtagcgaa ctggcgatgg atttttgtcg tgcaggcaaa gcggtcacgc taatcgacaa
    16621 cgctgccagt attctggcgt cattaatgcc accggaagta agcagccgct tgcagcatcg
    16681 gttgacggag atgggcgttc atctgttgtt gaaatctcag ttgcaggggc tggaaaaaac
    16741 agattctggc attctggcaa cgctggaccg ccagcgcagt atcgaagtgg atgcggtaat
    16801 tgccgccacc ggactgcgcc cggaaaccgc cctggcacga cgcgccgggc tgacgattaa
    16861 tcgcggcgtt tgcgtcgata gttatctgca aaccagtaat gccgatattt acgcgctggg
    16921 cgattgcgcg gaaattaacg gtcaggtatt gccgttcctc cagccgattc aacttagcgc
    16981 gatggtgctg gcaaaaaatc ttctcggcaa taacacgcca ctgaaactac cggcgatgct
    17041 ggtgaaaatc aaaacgccgg aattaccgct gcatctggca ggcgaaaccc agcgtcagga
    17101 tttacgctgg cagattaata ccgaacgcca gggaatggtg gcgcgcggcg ttgacgatgc
    17161 tgaccagctt cgcgcctttg tggtcagtga ggatcggatg aaagaggcat ttggattgtt
    17221 gaaaacgttg ccgatgtagg tgggctactg tgcctaaaat gtcggatgcg acgctggcgc
    17281 gtcttatcca acctacgggg agcgcatgtg ttggtcacat actgcgttca tgtcgcatcc
    17341 acctactgtg cccaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac
    17401 gcatgtgtag gccggataag gcgtttacgc cgcatccggc aatggtgtcc aaatgcaaca
    17461 cgttttatcc gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc
    17521 ccagagacaa accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag
    17581 cgagataatg cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata
    17641 ccagcgtagt gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg
    17701 ccagcgcatc atgaaacgcc cacgcgcgtt gattaaccgg tgcctgccag ttcagccact
    17761 gctgccagaa agtggcgaga tccagttgat tgtccaccag cggcattgtc accggatgcg
    17821 tcactccgtg gcacgaagct gcgagtgcct ccagagcaca agccgcttca ccttcataac
    17881 ttaacgtggc tggcgcacag cccagtgccg ccgccacggc atcgaacaac cgcccacacg
    17941 atgacgccag cggcgcgtta attccacgct caatggcccg cgccagcacg ctccagtttt
    18001 gctgttgcac acttgctgtt tcagaataat tctgccactc cggcacaaag cgcaggcact
    18061 gcgccagcag gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg
    18121 caggcaagcc gcccaggtgc tcacattcgc gatagttcac ccgcagacac tcgccgcccc
    18181 acaaagcgcc gttctccccc ataccgatac catcgagcgt caaagcaatg acatccccgc
    18241 catccagcgg ccactgatgc tctgccagac acgccgctgc gtgggcatga tgatgcagca
    18301 ccgtttgcgt cggcatattc atttcgctcg cccactggct ggagacatag cccggatgca
    18361 cgtcatgcac aacgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt
    18421 cgcgccactg catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg
    18481 cttgttcacc gcgcaccagg cagaaggtgt ttttcagatc cgcgccgaga cacagcacag
    18541 gcggaacatt tttaaagccc ggaggcaaag ccagcgcatc cggcacatac ccccgcgaac
    18601 ggcgcagcat ttcgccgctt tcgcgcacca ccgaatcatc catccgctgc acgatgtcgc
    18661 ggttatgtat caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc
    18721 tgatagctgg tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt
    18781 cctgtaacag caaatgctgg agcgggttcg caggcaacat taccccgact tcgttaaggt
    18841 caggggcgat atcatcacaa agctcaggaa cgtatttttt atccaccagc acaatcggcg
    18901 cggcgggcgt ggtaagcaac tggcgcgcag cgtctggtaa accgtcagcc actggcaaca
    18961 tgactgccag cggtttcgcc gggcggtgtt tgcgctcccg cagtgtcgcc accgcgttac
    19021 tgttacgtgc atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaattttgc
    19081 ccatttttaa ctgtgcgata gctgcctgta atgccgcctc ttgttccgca tgttcaccat
    19141 gacttaccca ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc
    19201 gacgatcgag cgggtcgcgg tactctttat cacaggctgg acacagcgga aactccgcca
    19261 ttacggtaaa cgggcggtcg taaggcatgg cgcgaataat ggtgaaacgc gggccgcagt
    19321 gggtacagtt gataaacgga taacgataac gccgttcgcc tggggtattc atttcggcaa
    19381 ggcaagcagg gcaagtagcg gcatccggga caatttgcgt attcatggca cctcccgcgc
    19441 tctggcggat agtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc
    19501 tatcaatacg cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt
    19561 cttccagcag ccggacttct acgccatcgc cgtcattaca gacatcgccg tgaagattta
    19621 attgctgtgc cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa
    19681 tacgcagttg gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa
    19741 ttgcgtaacc cgtccagccg gacacgacag cgtcgcatcc ggcagtcaca ggtcggcgat
    19801 accgcccgct tcgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct
    19861 gtaagatttt tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag
    19921 tttattacga tcgacacaaa ttagcgcatg ggtcgggcaa gccgccatac acgccgggcc
    19981 gtcttcacga tggttgcaca ggtcgcattt attggcttca gctttgtcag cccgtacatt
    20041 cagccccacg ccgctgttgc gaatcaccgg acgtaccacc acttccatcg cgccatacgg
    20101 gcaagccacc acgcaggttt tgcaaccaat acaacgttcc tgcatcacat gaacaaaccc
    20161 tttatcacgg ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg
    20221 acggcaaact gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa
    20281 tgtttccggg gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac
    20341 ttcacaggta cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa
    20401 attctccagc aatgacagtt aatgcgccga tacattcaca aatcatgcca gtttttaatt
    20461 tactgttatt taaggaaatt aatttctgta atgcaggaaa aacgatgtca tcgacactag
    20521 tgacgatgac atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcaga
    20581 tcaattttcc gctgaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca
    20641 gccgtccaat aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg
    20701 gaacgatata gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca
    20761 ccgctacgcc gcgctcgtgc aacgctttca tcgcacctat cgccatatcg tcgttactgg
    20821 caactaacgc gctaaattta gccccacgtt cgagcaacat ttctacccct tcggccccgc
    20881 tggcaggcgt ccatctaccg ttagcgataa gtttttcatt gagcgcaata ccgtgctgcg
    20941 ccagcgcgtc tttatatccg gcaagacgtt caacgctggt gggagaatcc atcgaaccgg
    21001 taaggaaagc aatctcctga tgtccggcgt ttatcaactc tgccacggcg ttaaaactgg
    21061 tctgtttatg atcgcaccag acgctatggc tgctgttttt gcgcaggcgg cgattaagca
    21121 ccattatcgg ctgactgtgc gcgtcaatga tgtcatcgat ctcatccacg cttaaaaaac
    21181 gcgggtaaat cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct
    21241 cttcttcggc gctgtgttta ccatctgcca atagcaactg ccgccctttc tcttccgcca
    21301 ttcgcgcggc atgaaacagt aattcactaa aataaatgcc gtggtaaagc gtgttggtca
    21361 ctaccagccc cagcgtctga gtactcttcg ccgacaaatt gcgcgccagc aagtttgggc
    21421 ggtaaccgct ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc
    21481 cattacctga aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt
    21541 ccagcatcgt cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg
    21601 tttgccgatt gtccttgcgc aatcggcggg aaaaatattc aggtgaccga tttcacaaat
    21661 ataaaaaatg aacaattaac tctcttgctt atttagtgac aactattcat gattttgtga
    21721 aaccggtttc ctaattccgt ttcagcatcg gcatttttcc gttacgtcga ctgataacaa
    21781 ctacatctac cctactgata acaggataaa atccgatggc caaaaattat gcggcgctgg
    21841 cacgctcggt gatagcggca ctgggcggcg ttgataacat ctcggcggtc acgcactgta
    21901 tgacgcgttt gcgctttgtt atcaaagatg accagcttat cgacagcccg acgttaaaaa
    21961 ccatccccgg cgtgctcggc gtggtacgta gtgacaacca gtgtcaggtg attatcggca
    22021 ataccgtttc acaagccttt caggaagtcg tcagcctgct gccgggagat atgcagcccg
    22081 cacagcccgt gggtaaaccc aaactcacgc tgcgtcgcat tggtgcaggg atcctcgatg
    22141 cgctgatcgg caccatgtca ccgctgatcc cggcgattat cggcggatcg atggtcaaac
    22201 tgctggcgat gatcctcgag atgagcggcg tgctgacaaa aggatcgccg accttaacca
    22261 ttctgaatgt gattggtgac ggtgctttct tcttcctgcc gctgatggtc gcggcatctg
    22321 ccgccatcaa atttaaaacc aatatgtcgc tggcgattgc catcgcgggt gtgctggtac
    22381 atccgagttt tattgaactg atggcgaaag cggcccaggg tgaacatgtc gaatttgccc
    22441 tgattccggt caccgcggtg aaatacacct acacagtgat cccggcgctg gtgatgacct
    22501 ggtgcctgtc atatatcgaa cgctgggtgg atcgcattac accggcggtg accaaaaact
    22561 tcctcaagcc aatgctgatt gtgttgattg ccgcaccgct ggcaatcctg ctgattggcc
    22621 cgattggtat ctggatcggt agcgccattt cggcgctggt ttacaccatt catggttatc
    22681 tgggctggct ttcagtcgcc attatgggcg cgctgtggcc tctgctggta atgaccggga
    22741 tgcaccgcgt ctttacgcca accatcattc agaccattgc cgaaaccggc aaagaaggga
    22801 tggtcatgcc gtcagagatc ggcgctaacc tgtcgctggg cggttcatca ctggcggtgg
    22861 cgtggaaaac gaaaaacccg gaactgcgcc agacggcgct ggctgcggcg gcatcagcca
    22921 ttatggcggg gatttccgaa ccggcgttat acggcgtggc gatccgcctg aaacgtccgc
    22981 ttatcgccag tcttatcagc ggctttattt gcggcgcggt tgccggtatg gcggggcttg
    23041 ccagccactc aatggcagcg ccggggctat ttaccagcgt gcagttcttc gatccggcga
    23101 atccaatgag catcgtctgg gtgttcgcgg tcatggcgct ggcggtcatg ctgtcgttta
    23161 tcctcacact gttgctcggc tttgaggata ttcctgttga ggaagcgact gccgaggcgc
    23221 gaaagcatca gagcgtacaa ccgaccgtcg ccaaagaagt aagtcttaat tgaggatgaa
    23281 aatgtcagta tttccagaag gttttttatg gggcggcgcg cttgccgcca accagtctga
    23341 aggtgcgttc cgtgaaggtg gcaaagggct gaccactgtc gatatgatcc cacacggcga
    23401 gcatcgaatg gcggtgaaac tggggctgga aaaacgtttt cagttgcgcg atgacgagtt
    23461 ttatcccagc catgaggcga cggattttta tcatcgttat aaagaagata tcgccctgat
    23521 ggcagagatg ggattcaagg ttttccgtac ctcaattgcc tggagccgtc tctttccgca
    23581 gggcgatgaa atcacgccca atcagcaggg cattgctttt tatcgttctg tctttgaaga
    23641 gtgtaaaaag tacggtatcg aaccgctggt cacgttgtgc cacttcgatg tgccgatgca
    23701 tctggtcacc gaatatggct cctggcgtaa ccgcaagctg gtggagtttt tcagccgcta
    23761 cgcccggacc tgctttgaag catttgatgg tctggtgaaa tactggctta ccttcaatga
    23821 aatcaacatt atgttgcata gcccgttctc cggcgcgggt ctggtgtttg aagaaggtga
    23881 aaatcaggat caggtgaaat atcaggccgc gcatcaccag ctggttgcca gtgcgctagc
    23941 caccaaaatc gcccatgagg ttaacccgca aaatcaggtg ggctgtatgc tggcgggcgg
    24001 taacttctac ccttacagtt gcaagccgga agatgtctgg gcggcgctgg agaaagaccg
    24061 ggaaaacctg ttttttatcg atgtgcaggc gcggggcgcg tatccggctt actctgcccg
    24121 cgtattccgc gaaaaagggg taaccatcga caaagcaccg ggcgatgatg aaatcctgaa
    24181 aaacaccgtc gattttgtct ctttcagcta ttacgcctcg cgctgcgcct cggcggagat
    24241 gaacgccaac aatagcagtg cggcgaacgt agtgaaatcg ctgcgtaacc cgtatctgca
    24301 agtgagcgac tggggctggg gtattgatcc actcggtctg cgtatcacca tgaacatgat
    24361 gtacgaccgt tatcagaagc cgctgtttct ggtggaaaac ggcctcggcg caaaagatga
    24421 acttgctgcc aatggcgaga ttaacgatga ctatcgcatc agctatttac gcgaacatat
    24481 ccgcgcaatg ggcgaagcga ttgcagatgg cattccgctg atgggctaca ccacatgggg
    24541 ctgtattgat ttagtttccg cctctacggg tgaaatgagc aaacgctacg gttttgtcta
    24601 cgtagaccgt gacgacgcag gcaacggcac gctgacgcgc acacgtaaga aatcgttctg
    24661 gtggtataaa aaagtgattg ccagtaatgg tgaagattta gagtaagcaa cagtgccgga
    24721 tgcggcgtga acgccttatc cggcctacat gtcgcacgaa ctaggaaggc ccgataagcg
    24781 cagcgcatcg ggcaatctgg ggtttggcat cagtcaaaat caaagaaacc attccggctt
    24841 aacgccaaat cgggctgaaa gtgccttaat gtgggatatc gtcagggatc gttgtccaga
    24901 caaaatctga ctgaccaggg atttagagcc gatttcgttc ttcaaatccg cataggtcag
    24961 attatgttga tcaatcagcg tgcgaagtaa cgccacgcca acaggcattg ccgcaacggc
    25021 tttgtcgaac tctgcaaact tttcgttgtt gttttcatac tctgcaatgc ggctcgccag
    25081 aaagtcgata agtggattgg tgtcatcttc ttcaatcaaa tattcgacca gttccagtgc
    25141 ttcacgataa tcatcttctg agtcactgcc tcccagaaag gggactgcat tgaccagttc
    25201 tcttgttgct ttaacagcgc gtgcagcatt agcagtcatt ctttgttctc tctgtagtaa
    25261 cgtgtcagtt tgtcatactc tgcgtgattc gttatgtgct tcacaaagaa ccgcttatta
    25321 acaaagttta tgtaggcaat aaccctaaga ttgttcccgc cgacatccag aacgtaccat
    25381 ttgttgcgat atctaaaatt gtcgaggttg ggaaacgccg ttcgcatttc ttctggtgta
    25441 gaaaaatccg tttccttaat aacccgatac aatgcctgat gagccagagc gtcgttagga
    25501 tatttacgcg cagactcttc aaatggcgct ttagaaatga tgtgcatttt taagttttaa
    25561 cttccttata gtaagtaaac agccacatct gcgatgttct atccttatgt gttcggaaac
    25621 tatagctcat caaatccaag ttcacaatat gtaaacactt gtgcgcgata taattacccc
    25681 caaaatcctc atagccactc tgcaaaaagt cagttctggt tcacccctca agcacatccc
    25741 cctttatcaa gcaacgctgc gtctgcgcca gatcttccgt ttgcgggtcg cgcccggcga
    25801 tgggtttgag gacgaattta cggttggggg tataagtaaa acgcgtgagc gcgggatagc
    25861 ttaacttgcc aaaccagccg tgaacacgct gccatttggc gtcttttacc tgatacagac
    25921 tggttatcca gtaaccagaa ttaaactgca tatccagcaa ttgcgtgtga ccattgcctt
    25981 gcaaatccag caaatcatct acgccagttt tgcttgcggt gtagaatccc ctgggttcaa
    26041 agacacaggg acggccttcg cgcgtgaacg tcataagaat taaatgtgca taaggagcca
    26101 gaccattacc ggtacttggt aaccagataa ccagatcctg aatgccgtta cgatcaagat
    26161 cggcgcgata gagttgccct ttgcccgcta tgtttgccag catacccaca ggcacagtcc
    26221 aggatttacc ggcgcggtct ttagcggtta attgccagta atcttcttca tttggcatct
    26281 tctcaagcgt cacgccagca acgcccactt tcctaaaatg ccaggtttgc tcggcatcat
    26341 aaggcatggt caggagaggt ttcagcgccc gcttttgcgc gtattcgctg tcactcattg
    26401 gcagatattc ggtaaagctg gtggcctgtg caggcagcac taagctcaat aaaaaagcgc
    26461 acatccctgt tctgatgttc aattccctga attccctgat ggagaattat tcttcttcca
    26521 ccgctaactg cgcaaatcca ccatttcctt cccagccttc cagtcgttga taaacggttt
    26581 ctaccgcatc tttaatcggc tgggtcatcg ggtagtaaaa gccgacgata tccggctgaa
    26641 tgccgaggaa aatcacttca ccaatgtctt cttttaactg gtcgataagg taattcaacg
    26701 gcatgttatg ggtagtcatc ataaacatct cggcgatatc atccgggtcg atgatgcgga
    26761 tttcgccggg gtttagcccc atatccgtgg cgtcgacaat cagcagtcgt gtcgggcgca
    26821 gttcacggat ggcgacgatg tcgttttccg gtgcactacc gccgtcaatc accacccagt
    26881 tacctttcgg cgcggcggcg cacttttccg ccagcagcgg gccagcgcca tcatcgccca
    26941 tcatgctatt gccaacacag agtaaaacgt cagtcacgca gtctcctcac catcaaatag
    27001 atggcgtttt cctgatgaat atcatgcagc atacttaaca gcattgtact ccattccttc
    27061 tgctgcgaag tttgcgctgc ccgggcttta tcgaatgcgt tggccagcat cggcacatgg
    27121 ttaatgtcga tgacgatctc gccatatttc ggtacgcctt ccattttgcg gcgggcttcg
    27181 ctgcccgcct ccagagtggc aatccatgcc agatattcat cccatgggca ggtgagcgcc
    27241 gcttccaggc aatcgataac cccaaggtgg tgaccaatcg ccaggctgta atagaccacc
    27301 tgctgcgcct cggcgggcgt ggcatcgttc tcatcaataa atttacggct cagttgactg
    27361 aacaccacct tttcactcat cggatacgcg cctcttcaac aacatgattc agatggctga
    27421 caatctcgtt cagacgcgga tcgttttccg cttccagcca gcgtgcaacc tgttcttcgc
    27481 cctgccctaa ctgtgtaagg taatcatcgg caatctgacg accgtaacga taacccgcca
    27541 ggcgacgtgc ttcgcgatcc actttcacgc gcagcggctg caccatatcg ccatgcagaa
    27601 tctcagccgg ttgttcatcc tgctcacccg ggccacgggc gtgaattttc tgctccagca
    27661 ggccgagtgc cattgcaaag ccgtacagcg tggcggcagg cgttggcggg caacccggaa
    27721 tgtagacatc taccgggacg attttatccg taccgcccca cacgcagtag agatcgtgga
    27781 aaataccacc gctgttaccg cacgcaccgt aggagataca aattttcggg tccggcgcgg
    27841 actgccacgc acgcagcgca ggggatcgca ttgcacgggt gaccgcgccg gtaaacagta
    27901 aaatatccgc atgacgcggt gaaggaacga ctttaatgcc gaagcgttct gcatcaaaca
    27961 gcggcgataa agtggcgaaa atttcgattt cgcagccgtt gcagccgccg cagtccacgc
    28021 ggtaaacata ggcagaacgt ttgatttttt tcagtaacga cgccttcatg ctggcgatgg
    28081 attcatccac cgtcatcggg accggaatgc cgttggcgtc acgggggcct aataaattgc
    28141 tcatcagatg gcctctttca tatggcgagt cagttcaata cggtcggacg gcaccaggca
    28201 tttctggcgc ttacattccg ggcaagtctc aaagctttcg cggtggtttt ccgcgcggct
    28261 gtcaccattg tgcttaagca gcgcaatggc gtagtcgatc tctttctgga cggcgaaagg
    28321 acgattgcag acgcgacagc tgcacagcac aaaacgggac tgttgcagga aatcttcttt
    28381 cttccacacc gccagttcgt actcttgcga cagtttgatc gctgccgtcg ggcagacttc
    28441 ttcgcagcgt ccacagaaga tgcagcgccc gagattaaac tcccacgcca gctcaccggt
    28501 ggcgaggtca gtttcaaccg ttaaggcgtt tgacggacag gcattgacgc aggccgcaca
    28561 gccgatgcac tgctgcgggt tctgctccgg cttaccacgg aagtttttat caaccgcaat
    28621 cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt tgataaaggt
    28681 aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt aacgctcgag
    28741 ttctttgtac ggcaccactt tgctcttttt cttacgcaca tcgaccacgg tcatgcggtc
    28801 ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatcgg aaacggtgtt
    28861 accgcgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac ggcagcgcca
    28921 gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc cgcgcggcgc
    28981 ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca ccatcagcgg
    29041 accgcccggc aggttatcca gaccgtagtc gatcatgttc agcgcggtat aaacttcgtt
    29101 gatacgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga cttccattgg
    29161 cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcgt gaccgctggc
    29221 gcgaaccatc gggccaacgt tactgaaatc gcgggcgatt tccgggtcca gacgaccaat
    29281 gccgacggtg cgctgttcca tgttcggcgt actcagcagc acatccacca gctcctgcac
    29341 ttcacgacgc atctgttgcg ccagctggcg ggtctggatc atgtcatctt tcaacagatc
    29401 gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg tgaggatctc
    29461 tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg agtcaaagcc
    29521 ggtaaagtgg caggcgagac cgaggttgag cagatgcgag tgcaggcgtt ccacctccag
    29581 cagaatggcg cggatcatct gcgcacgttc tggcacctga atacccatcg cgttttctac
    29641 cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac ggtcagagag
    29701 gaaggtcact tcgttgtaac ccatacgggt ttccgccagt ttttccatgc cgcgatgaac
    29761 gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca gacggaagtg
    29821 gcccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt ttttcttgtc
    29881 gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg gacgctgacg
    29941 ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat ccggcagcac
    30001 cagacgacgt tcatccggca gaccaaccgg aatcaaaccg tacatatcgc gcacttcgcg
    30061 ctcgccccac accgccgccg gaacgcgcgg cgtcacggac ggatactccg gtttgttggc
    30121 gtcaacttcg acgcgaaccg ttacccaaca cttggtgccc ttctccatcg acagcacgta
    30181 ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca ccgacagcca
    30241 gccaccctgc ttgtagtaga gaaactccac cacttccggc aggtagttca ccttcacggt
    30301 cacggtcagc tgatctttgg tctgccaggc gtggtccagc acgacgcccg gaaatgcctc
    30361 attcagcgcg gcgagataat gttgacctaa tttttcttca gacatgctca aactctcttt
    30421 aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc agcccaggta
    30481 atgcgcggag taatatcaag acgcagacgg gccatgctgt tttcgaacag cgcgataacc
    30541 aggacgccga cgaccagttt gacgatggca atcaccagcg ccagcagcag cccaccggcg
    30601 gtgaaggttt ccatctgtcc ccacgggata aatacgccga cgaacatctg cagcaccacc
    30661 agctgtttca ggctgatacc ccatttcatg acgccaaagc cgctgccgct gtattcagag
    30721 agcgggcctt cctgcagctc ctgctcggct tccgccaggt cgaacggcag tttgcccatt
    30781 tcgataaagg tggcgaacgc acaggcacaa agcgccagta ccagcgggat gctctggctc
    30841 agcggccagt gataaacggt gtcggtgatg ttgctgatgt tggtggaacc ggcaacctgt
    30901 gcggcaaccc acagaccaag cagcagcatc ggttcgacca gcacgccaag catcgcttca
    30961 cggctcgcgc cgatagcggt aaacgggcta ccggtatcca gaccagaaat ggcaaagaag
    31021 aaacgcgcga tggcaaagag atacagtaag gtgattaaat cacccagttg cggcagcgga
    31081 gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca gcatgacgcc caccatcaca
    31141 tacggcgtca ggcggaacac ccagccggag gcatccgggc caacgctctg gcggcccagc
    31201 agtttgataa tgtcacgata ctcctgcaac acacccggcc cacgacggtt atgtaagcgg
    31261 gctcgcgcca cgcgggtaat accggagagc agcggcgcaa cggcaaatag caccagcgcc
    31321 tgaattaacg gatataaaac actcattctc aggctcctcg tgaaacaata atcaccacca
    31381 gtaccgccag ttcaaccagc gccatccggc ggaacagcaa cgcactcccc tcgcactgcc
    31441 agcccggcac cagagacacc ggattcagcc atttgcgtag tttcagcacc ggcgcaaacg
    31501 cctgtttcac cggcatggca aaaccgtgag cggtaatcac cattgatttt tcgtggtcgt
    31561 agccgcacac ccaggccgcg ccgcgggaac gcgatggcaa acgatcgcct ttgcaaatcg
    31621 ccataatgat gaatggcagc agcgggcagg caatcagcag caatgtgatc attggttgag
    31681 aaacggtggt gttagcaggc tccagcggca gaggtacagc agcagacagc atcggcagca
    31741 gccacggcgc agcaacaccg ccaattacgc agcaaatcgc cagtgccact acgcttaccc
    31801 tcatcaggag cggcgcacag gtggcgtttt cggcttcttt ggtacgcggc gcgccgagga
    31861 acgtgacgcc atagactttc gccatacaca tcaccgccag cgcaccggta attgccagac
    31921 ccacagcgag cagcggcccc agcagacggg caacaaacgc gccgctattg ctcagtttga
    31981 agaaggattg atagataacc cattccccgg caaacccgtt cagcggcggc agcgcagcca
    32041 ttgccattaa cccgactaac atggcgatgg agataaccgg cattttcttg ccaataccac
    32101 cgagtttttc gatatcgcga tgaccggtac ggaaccagac gctccccgcc ccgaggaaca
    32161 gtacgctttt gaacaggcta tggttaagca gatggtacag accaccgacc aggccaagag
    32221 caatcagcgc cggttgttcg agcgcgatac ccgttacgcc agcgcccagc cccagcagga
    32281 taatgccgat attttccagg gtgtggtaag ccagcaggcg ctggatgttg tgctccatca
    32341 gcgcatacag accaccgaca aacgcggtga tcatgccgag caccagcagc gcgatccccc
    32401 accacagcgg tgcattaccg cccagcagtg acagggttaa aatgcccagc aggccaattt
    32461 tcatgactac cgtagaaaac aacgcggcag ctggcgcaga ggcgttagca tgtgcctgcg
    32521 gcacccagcc gtgcagcgga ataatcccgg cgagcaggcc aaagccaatc acaccgagca
    32581 gccagatatc ggaaccgagc ggcagctgtt gcatacgcat atccagcagg cgcagatcca
    32641 gcgtgccata acgctgccac agcagccagc aagcaatcgc cagcagcaga gtgccaagac
    32701 gccccagcgc aaaccacagt ttgccctctt tgctgttgct agtgaggaac accgcgcaca
    32761 gggccatgat ttcggccatt accacgaaca tgccgaggtt gctggcaatg acggcgcaga
    32821 cggcggcagc catcaacata ttgatctgca agccgttgca cttcacctgc gcgtggcgat
    32881 gccagtcaat gttatagagg ctgacaaaca gaccgcaaag gccgagggta atcagccaaa
    32941 tcgcgttaag cggggagatt tgcacatcgt agcttaccag cgacagcgca ccgctcacgc
    33001 caaccgcgcc agtcagcaca gtgaagcccg cggctgccgt atacaggcta ccaaccgcgc
    33061 cgccaattcc ggctatccag ccacttagcg tcttttgaaa agaaaagaga aatgccagaa
    33121 cagcggcggc gacaaaccac gccacgccgc tattgatcag ggaaattgcg ctcatttagc
    33181 ctctccactt tgagcctgct gaaacaaggt gagatcgcca aagtccgtgt taaaggtcag
    33241 ctcacgctta cgtttgctga cgcgggcgat atcggtgtta tccaccagat gcagggcttt
    33301 agtcgggcac atccgcacgc aggccggacc ttgttcatca aagctacaaa ggtcacattt
    33361 gacggcgatc gcgcgaatac ctggcaccca gtcaagcaat gtgctgacac gcgccggagc
    33421 aggcggtgcc ggtggcgctt tcggggtatt ggcgtttgcc ggaatatcca gcggacggct
    33481 gccggaaaat tcaattgcgc caaacgggca ggcgatgccg cacagcttgc agcttacgca
    33541 caggctttca ttcaactgca cggccccatc aacgcgggtg atggcgttaa ccgggcagac
    33601 caccgcgcag ggtgcatctt cacagtggtg acagagctgc ggcgcagatt ctttttcatt
    33661 cagcatcact ctcaggcgcg gcattgattg caggccgtgc tggcgatgcg tctctgaaca
    33721 ggcggcctca caagtgtggc agccgataca gagcgtggag tcagcaatta caaaacgatt
    33781 caccaggcat tcctcaggtg attgtcattt ttgacgaaaa catgccgatg aaatgtcatt
    33841 ttcgacactc atcgacacgc ccatccccaa acaggcgtaa cgcctgcaaa acgggcaaag
    33901 cctcagctca tgctgccggg ctttgtccct ttaccagttg gcttaaattc accggcacat
    33961 tgttttcaat agcggtgtat aagctgtcgt aaacgccagc gattttttcg agatagtgtt
    34021 ccagaacctg ttcgcgatcg cggatgttga ccttgccatc aatcatgccg tcaatgctca
    34081 gttgcgcctg cgcaatccgc tgtttgtctt cgccatcttt tgtttcgacg taatactcga
    34141 tggtgtggct gttaaagcca tccgccaggg tgacgtaaat gcgaaaatgt tcaaaaagga
    34201 cgaaacagag ttctttgtcc ggcgcgcagc tcatggcgtg atgcaggcgc gctttcgata
    34261 acgtgatccc ctgaaccagc gaattgcagt agatgtgcca ctggtcctgt aggcgacgat
    34321 gccgctgtgc gatgtaatcg gctttctcgc ttatttccca aatagtcatt gtcaggttac
    34381 ccgtttaaca gagatgccag ctttaagcat tttctgtgcc aacttttaat ttattgttat
    34441 taaagagatt tttaagctaa agatgaattt cgtcgccgtg tcgacgtgtc atttcgacat
    34501 catcgacatt attcaccgca gggataatca acactggcac aattattgct tgtagctggc
    34561 aatagttaat gggaggcgat atgcacgaaa taaccctctg ccaacgggca ctggaattga
    34621 tcgaacagca ggccgcaaaa cacggcgcaa aacgcgtaac tggggtctgg ctcaaaattg
    34681 gcgcattttc ttgtgtcgaa accagctctc ttgccttttg ttttgatctg gtttgccgcg
    34741 gcagcgtggc ggaaggttgt aaactgcacc tcgaagaaca agaggccgaa tgctggtgtg
    34801 aaacatgcca acagtatgtg acgctactga cccagcgcgt ccgccgctgt ccacagtgtc
    34861 atggtgacat gctgcagatt gtggcagacg acggtttaca gattcggcgg atagaaatag
    34921 accaggagtg agcgatgtgt acaacatgcg gttgcggtga aggcaacctg tatatcgagg
    34981 gtgatgaaca taaccctcat tccgcgtttc gtagcgcgcc atttgccccg gcggcacgcc
    35041 cgaagatgaa aatcaccggc attaaagcgc ctgaatttac ccccagccag actgaagaag
    35101 gcgacctgca ttacggtcat ggcgaagcgg gcactcacgc accgggcatg agccagcgtc
    35161 ggatgctgga agtcgaaatt gacgtgctgg acaaaaataa ccgtctggct gaacgcaacc
    35221 gcgcgcgctt tgctgcccgc aagcaactgg tgctcaacct ggtttccagc ccaggttccg
    35281 gtaaaaccac cctgctgacg gaaaccttaa tgcgcctgaa agacagcgtt ccgtgcgcgg
    35341 ttattgaagg tgaccagcaa accgtgaacg atgccgcacg cattcgcgct accggcacac
    35401 cagcgattca ggtgaacacc ggtaaaggct gccatcttga cgcacagatg attgccgacg
    35461 ccgcaccgcg tctgccactg gacgataacg gtattctgtt tatcgaaaac gttggcaacc
    35521 tcgtatgccc ggccagcttc gatctcggtg aaaaacacaa agtggcggtg ctttccgtta
    35581 ccgaaggtga agacaaaccg ctgaaatatc cgcatatgtt tgccgccgcc tcgctgatgc
    35641 tgctcaacaa agttgacctg ttgccgtatc tcaactttga cgttgagaag tgcctcgcct
    35701 gcgcccgcga agtcaatcca gaaattgaaa tcatccttat ttccgccacc agcggtgaag
    35761 ggatggacca gtggctgaac tggctggaga cacagcgatg tgcataggcg ttccaggcca
    35821 gatccgcacc attgacggca accaggcaaa agtcgacgtc tgcggcattc agcgcgatgt
    35881 cgatttaacg ttagtcggca gctgcgatga aaacggtcag ccgcgcgtgg gccagtgggt
    35941 actggtacac gttggctttg ccatgagcgt aattaatgaa gccgaagcac gcgacactct
    36001 cgacgcctta caaaacatgt ttgacgttga gccggatgtc ggcgcgctgt tgtatggcga
    36061 ggaaaaataa tgcgttttgt tgatgaatat cgcgcgccgg aacaggtgat gcagttaatt
    36121 gagcatctgc gcgaacgtgc ttcacatctc tcttacaccg ccgaacgccc tctgcggatt
    36181 atggaagtgt gcggtggtca tacccacgct atttttaaat tcggcctcga ccagttactg
    36241 cctgaaaacg ttgagtttat ccacggtccg ggttgcccgg tgtgcgtact gccgatgggt
    36301 agaatcgaca cctgcgtgga gattgccagc catccggaag tcatcttctg tacctttggc
    36361 gacgccatgc gcgtaccagg gaaacaggga tcgctgttgc aggcaaaagc acgcggtgcc
    36421 gatgtgcgca tcgtttactc gccgatggat gcgttgaaac tggcgcagga gaatccaacc
    36481 cgcaaagtgg tgttcttcgg cttaggtttt gaaaccacca tgccgaccac cgccatcact
    36541 ctgcaacagg cgaaagcgcg tgatgtgcag aatttttact tcttctgcca gcacattacg
    36601 cttatcccga cgctgcgcag tttgctggaa cagccggata acggtatcga cgcgttcctc
    36661 gcgccgggcc acgttagtat ggttatcggc actgatgcct ataattttat cgccagcgat
    36721 tttcatcgtc cgctggtggt cgctggtttc gaaccccttg atctactgca aggcgtggtc
    36781 atgctggtgg agcagaaaat agcggcccac agcaaggtag agaatcagta tcgtcgggtg
    36841 gtaccggatg ccggtaacct gctggcgcaa caggcgattg ccgatgtgtt ctgtgtcaac
    36901 ggcgacagcg aatggcgcgg cttaggcgtg attgaatctt ctggcgtgca cctgacgccg
    36961 gattatcaac gattcgatgc cgaagcacat ttccgcccgg caccgcagca ggtctgcgat
    37021 gacccgcgcg cgcgttgtgg tgaggtatta acgggcaaat gtaagccgca tcaatgcccg
    37081 ctgtttggta acacctgtaa tcctcaaacc gcatttggtg cactgatggt ctcctccgaa
    37141 ggagcgtgcg ccgcgtggta tcagtatcgt cagcaggaga gtgaagcgtg aataatatcc
    37201 aactcgccca cggcagcggc ggccaggcga tgcagcaatt aatcaacagc ctgtttatgg
    37261 aagcctttgc caacccgtgg ctggcagagc aggaagatca ggcacgtctt gatctggcgc
    37321 agctggtagc agaaggcgat cgtctggcgt tctccaccga cagttacgtc attgacccgc
    37381 tgttctttcc tggcggtaat atcggcaagc tggcgatttg cggcactgcg aatgacgttg
    37441 cggtcagtgg cgctattccg cgctatctct cctgtggctt tatcctcgaa gaaggattgc
    37501 cgatggagac actgaaagcc gtagtgacca gcatggcaga aaccgcccgc acggcaggca
    37561 ttgccatcgt tactggcgat actaaagtgg tgcagcgcgg cgcggcagat aaactgttta
    37621 tcaacaccgc tggcatgggc gcaattccgg cgaatattca ctggggcgca cagacgctaa
    37681 ccgcaggcga tgtattactg gttagcggca cgctcggcga ccacggggcg actatcctta
    37741 acctgcgtga gcagctgggg ctggatggcg aactggtcag cgactgcgcg gtactgacgc
    37801 cgcttattca gacgctgcgt gacattcccg gcgtgaaagc gctgcgtgat gccacccgtg
    37861 gtggtgtgaa cgcggtagtt catgagttcg cggcagcctg cggttgtggt attgaaattt
    37921 cagaagcggc actgccggtt aaacctgccg tgcgcggcgt ttgcgaactg ctgggactgg
    37981 acgcccttaa ctttgccaac gaaggcaaac tggtgatcgc cgttgaacgc aacgcggcag
    38041 agcaagtgct ggcagcgtta cattcccatc cactgggaaa agacgcggcg ctgattggtg
    38101 aagtggtgga acgtaaaggt gttcgtcttg ccggtctgta tggcgtgaaa cgaaccctcg
    38161 atttaccaca cgccgaaccg cttccgcgta tatgctaata aaattctaaa tctcctatag
    38221 ttagtcaatg accttttgca ccgctttgcg gtgctttcct ggaagaacaa aatgtcatat
    38281 acaccgatga gtgatctcgg acagcaaagg ttgttcgaca tcactcggac actattgcag
    38341 cagcccgatc tggcctcgct gtgtgaggct ctttcgcaac tggtaaagcg ttctgcgctc
    38401 gccgacaacg cggctattgt gttgtggcaa gcgcagactc aacgtgcgtc ttattacgca
    38461 tcgcgtgaaa aagacacccc cattaaatat gaagacgaaa ctgttctggc acacggtccg
    38521 gtacgccgca ttttgtcgcg ccctgatacg ctgcattgca gttacgaaga attttgtgaa
    38581 acctggccgc agctggtcgc aggtgggcta taccccaaat ttggtcacta ttgcctgatg
    38641 ccactggcgg cggaagggca tatttttggt ggctgtgaat ttattcgtta tgacgatcgt
    38701 ccctggagcg aaaaagagtt caatcgtctg caaacattta cgcagatcgt ttctgtcgtc
    38761 accgaacaaa tccagagccg cgtcgtcaac aatgtcgact atgagttgtt atgccgggag
    38821 cgcgataact tccgcatcct ggttgccatt accaacgcgg tgctttcccg cctggatatg
    38881 gacgaactgg tcagcgaagt cgccaaagaa atccattact atttcgacat tgacgatatc
    38941 agcattgtct tacgcagcca ccgtaaaaac aaactcaaca tctactccac tcactatctt
    39001 gataaacaac atcccgccca cgaacagagc gaagtcgatg aagccggaac cctcaccgaa
    39061 cgcgtgttca aaagtaaaga gatgctgctg atcaatctcc acgagcggga cgatttagcc
    39121 ccctatgaac gcatgttgtt cgacacctgg ggcaaccaga ttcaaacctt gtgcctgtta
    39181 ccgctgatgt ctggcgacac catgctgggc gtgctgaaac tggcgaaatg cgaagagaaa
    39241 gtgtttacca ctaccaatct gaatttgctg cgccagattg ccgaacgtgt ggcaatcgct
    39301 gtcgataacg ccctcgccta tcaggaaatc catcgtctga aagaacggct ggttgatgaa
    39361 aacctcgccc tgaccgagca gctcaacaat gttgatagtg aatttggcga gattattggc
    39421 cgcagcgaag ccatgtacag cgtgcttaaa caagttgaaa tggtggcgca aagtgacagt
    39481 accgtgctga tcctcggtga aactggcacg ggtaaagagc tgattgcccg cgcgatccat
    39541 aatctcagtg ggcgtaataa tcgccgcatg gtcaaaatga actgcgcggc gatgcctgcc
    39601 ggattgctgg aaagcgatct gtttggtcat gagcgtgggg cttttaccgg tgccagcgcc
    39661 cagcgcattg gtcgttttga actggcggat aaaagctctc tgttcctcga cgaagtgggc
    39721 gatatgccac tggagttaca gccgaagttg ctgcgtgtat tgcaggaaca ggagtttgaa
    39781 cgtctcggca gcaacaaaat cattcagacg gacgtacgtt taattgccgc gactaaccgc
    39841 gatctgaaaa aaatggtcgc cgaccgagag ttccgtagcg atctctatta ccgcctgaac
    39901 gtattcccga ttcacctgcc gccactacgc gagcgtccgg aagatattcc gctgctggcg
    39961 aaagccttta ccttcaaaat tgcccgtcgt ctggggcgca atatcgacag cattcctgcc
    40021 gagacgctgc gcaccttgag caacatggag tggccgggta acgtacgcga actggaaaac
    40081 gtcattgagc gcgcggtatt gctaacacgc ggtaacgtgc tacagctgtc attgccagat
    40141 attgttttac cggaacctga aacgccgcct gccgcaacgg ttgtcgccca ggagggcgaa
    40201 gatgaatatc agttgattgt gcgcgtgctg aaagaaacca acggcgtggt tgccgggcct
    40261 aaaggcgctg cgcaacgtct ggggctgaaa cgcacgaccc tgctgtcacg gatgaagcgg
    40321 ctgggaattg ataaatcggc attgatttaa ctgcaaattg ccggacagat ctgcctgtcc
    40381 ggcatactat tcacgaggtt ttttcggacg atatttttcc ggcagttctg gcaccggacg
    40441 cttgtcatcg atgagatgac gcacggttaa gatcggatga cgccacagca ttctcggccc
    40501 ggcccaacgc ataatctgtt tcatctcttc acgctttgca ggctggtaac agtgcaccgg
    40561 acactgctta caggctggtt tctcttcgcc gaacacacat ttatccagcc gcttttgcgc
    40621 gtaaacaaac aacgcctcgt aatgctccgg ctccgctgac gcctgcgggc atttcgcttg
    40681 ataaagatcg atcatttttt taatcgtcag tttttcacga gagatacgct tgccggacat
    40741 gctgcctcca tactcttaag gtgtatttat attacaactt aatttgaaag gggactatgg
    40801 ctctgaagga ggaggtttat tcaaccgacg caattttgcg tacttgcttc ataagcatca
    40861 cgcaaaagct gcaaaacagc atctttcccg gaaccagcat caagaactcg cagttcgctt
    40921 cttcccctga aatgattaac tccggtatca tgtgcgcctt atgtgattac aacgaaaata
    40981 aaaaccatca caccccattt aatatcaggg aaccggacat aaccccatga gtgcaataga
    41041 aaatttcgac gcccatacgc ccatgatgca gcagtatctc aggctgaaag cccagcatcc
    41101 cgagatcctg ctgttttacc ggatgggtga tttttatgaa ctgttttatg acgacgcaaa
    41161 acgcgcgtcg caactgctgg atatttcact gaccaaacgc ggtgcttcgg cgggagagcc
    41221 gatcccgatg gcggggattc cctaccatgc ggtggaaaac tatctcgcca aactggtgaa
    41281 tcagggagag tccgttgcca tctgcgaaca aattggcgat ccggcgacca gcaaaggtcc
    41341 ggttgagcgc aaagttgtgc gtatcgttac gccaggcacc atcagcgatg aagccctgtt
    41401 acaggagcgt caggacaacc tgctggcggc tatctggcag gacagcaaag gtttcggcta
    41461 cgcgacgctg gatatcagct ccgggcgttt tcgcctgagc gaaccggccg accgcgaaac
    41521 gatggcggca gagctgcaac gcactaatcc ggcggaactg ctgtatgcag aagatttcgc
    41581 cgagatgtcg ctgattgaag gccgtcgcgg cctgcgccgt cgcccgctgt gggagtttga
    41641 aatcgacacc gcgcgccagc agttgaatct gcaatttggc acccgcgatc tggtcggttt
    41701 tggcgtcgag aacgcgccgc gcggactttg tgctgccggt tgtctgttgc agtatgcgaa
    41761 agatacccaa cgcacgaccc tgccgcatat tcgttctatc accatggaac gtgagcagga
    41821 cagcatcatt atggatgccg cgacacgtcg taacctggaa attactcaga acctggcggg
    41881 cggtgcggaa aatacgctgg cttctgtgct cgactgcacc gtcacgccga tgggtagtcg
    41941 tatgctgaaa cgctggctgc atatgccagt gcgcgatacc cgcgtgttgc ttgagcgcca
    42001 gcaaactatt ggcgcattgc aggatttcac cgccgagttg cagccggtac tgcgtcaggt
    42061 cggcgacctg gaacgtattc tggcacgtct ggctttacga actgctcgcc cacgcgatct
    42121 ggcccgtatg cgccacgctt tccagcaact gccggagctg cgtgcgcagt tagaaactgt
    42181 cgatagtgca ccggtacagg cgctacgtga gaagatgggc gagtttgccg agctgcgcga
    42241 tctgctggag cgagcaatca tcgacacacc gccagtgctg gtacgcgacg gtggtgttat
    42301 cgcatcgggc tataacgaag agctggatga gtggcgcgcg ctggctgacg gcgcgaccga
    42361 ttatctggag cgtctggaag tccgcgagcg tgaacgtacc ggcctggaca cgctgaaagt
    42421 tggctttaat gcggtgcacg gctactacat tcaaatcagc cgtgggcaaa gccatctggc
    42481 acccatcaac tacatgcgtc gccagacgct gaaaaacgcc gagcgctaca tcattccaga
    42541 gctaaaagag tacgaagata aagttctcac ctcaaaaggc aaagcactgg cactggaaaa
    42601 acagctttat gaagagctgt tcgacctgct gttgccgcat ctggaagcgt tgcaacagag
    42661 cgcgagcgcg ctggcggaac tcgacgtgct ggttaacctg gcggaacggg cctataccct
    42721 gaactacacc tgcccgacct tcattgataa accgggcatt cgcattaccg aaggtcgcca
    42781 tccggtagtt gaacaagtac tgaatgagcc atttatcgct aacccgctga atctgtcgcc
    42841 gcagcgccgt atgttgatta ttaccggccc gaacatgggc ggtaaaagta cctatatgcg
    42901 ccagaccgca ctgattgcgc tgatggccta catcggcagc tatgtaccgg cacaaaaagt
    42961 cgagattgga cctatcgatc gcatctttac ccgcgtaggc gcggcagatg acctggcttc
    43021 cgggcgctca acctttatgg tggagatgac tgaaaccgcc aatattttac ataacgccac
    43081 cgaatacagt ctggtgttaa tggatgagat cgggcgtgga acgtccacct acgatggtct
    43141 gtcgctggcg tgggcgtgcg cagaaaatct ggcgaataag attaaggcat tgacgctgtt
    43201 tgccacccac tatttcgagc tgacccagct accggagaaa atggaaggcg tcgccaacgt
    43261 gcatctcgat gcgctggagc acggagacac cattgccttt atgcatagcg tgcaggatgg
    43321 cgcggcgagc aaaagctacg gcctggcggt tgcagctctg gcaggcgtgc caaaagaggt
    43381 gattaagcgc gcacggcaaa aactgcgtga gctggaaagc atttcgccga acgccgccgc
    43441 tacgcaagtg gatggtacgc aaatgtcttt gctgtcagtg ccagaagaaa cttcgcctgc
    43501 ggtcgaagca ctggaaaatc tcgacccgga ttcactgact ccgcgtcagg cgctggagtg
    43561 gatttatcgc ttgaagagtc tggtgtaata acaattcccg atagtctttt gctatcggga
    43621 atattaacga caactgacga atcaaataaa aataccctgt ataataggaa agcttatttt
    43681 acagggtaaa accatgccat ctacacgcta tcaaaaaatc aatacccatc actatcgcca
    43741 tatatgggtc gttggtgata ttcatggtga atatcagtta ttacaatccc gcttacatca
    43801 actctctttt ttccccgaaa ccgacttact tatttctgtc ggcgataata ttgatcgtgg
    43861 gccggagagt cttgacgtcc tgcgcctgct aaaccagccc tggtttatct ccgttaaagg
    43921 caaccacgaa gcaatggcgc tggatgcgtt cgcgaccggc gatggcaata tgtggcttgc
    43981 cagcggtggt gactggtttt tcgatttaaa tgattcagag caaaaagaag ctacagatct
    44041 gttgctgaaa ttccatcacc ttccacatat tattgaaatc actaacgaca acataaaata
    44101 tgtcatcgca catgcagatt atccggggag tgaatatctc tttggtaaag aaatagcgga
    44161 gagcgaatta ctctggcctg ttgatcgtgt gcagaaatcg cttaatggcg agttacaaca
    44221 aataaacggc gctgattatt ttatatttgg acatatgatg tttgataaca ttcagacgtt
    44281 cgctaaccag atttatattg ataccggcac gccgaaaagc gggcggctgt cattttataa
    44341 aataaagtag tctcatgctt cttctgtgag gcatgagtaa ccaaatgtta ttgcaggcca
    44401 ttattcattt ttcgctacca gcaaagagag atcctgcttc gccagcgcgc gactggcact
    44461 ctccggcaaa ccgtcgtctg taataatctg atcaaactcg cttaatggta acgccagcca
    44521 tgtcgccacc tgaccatatt tcgtcgcatc acagaccaaa actcgctggc ggctggcact
    44581 ggcaatcgcc cgtttcaccg tgactttatc ttccgctggc gtagaaatcc cccgcacact
    44641 ccatgacgat gcagaaataa aagcctgatc aatcatcagg ctgcgcagca tggtcgcagc
    44701 ggcttccccg acacaggaac ggttttcccg acacactgca ccgccagtgt gaataattgt
    44761 gcaattactg ttgtcgagca agtagttcgc aataacgaaa tcgtttgtga ccacagtcag
    44821 tgactccatg tgaatcagat gctgtgctat cgctaacgtg gtcgttcccg catccagata
    44881 gatacaactt cccggctgaa caagacttgc cgccagcttg ccaatagccg ctttttgcgt
    44941 cattgccagc gcagttttta cctgatgaga aggttcatgc gccacgcgtc ccggagactg
    45001 gacgcctccg gacaccagca caacggctcc ctgctgctcc agtttttgta aatcccgacg
    45061 aatggtcata tgtgacacat tcattctgtc cgttagttca gcaatactga caatgccttt
    45121 ttcagctacc atctcaagga tgatttggcg acgctctacg ggtatcaact tttgctcctt
    45181 cctttgtcct gctgacattc tacgctattt gcctgcgaaa cgtgcgcggc gcaactaacg
    45241 cttagttcac ataaaataac acacaatgtt aatttatgtg aatcagctca ccatatcgtt
    45301 atcttccagc gcttatattc acaacatcaa acaaaatatc acttaaatta acaaggagag
    45361 cagatgaaaa cgggatctga gtttcatgtc ggtatcgttg gcttagggtc aatgggaatg
    45421 ggagcagcac tgtcatgtgt ccgcgcaggt ctttctacct ggggcgcaga cctgaacagc
    45481 aatgcctgcg ctacgttgaa agaggcaggt gcttgcgggg tttctgataa cgccgcgacg
    45541 tttgccgaaa aactggacgc tctgctggtg ctggtggtca atgcggccca ggttaaacag
    45601 gtgctgtttg gtgaaacagg cgttgcacaa catctgaaac ccggtacggc agtaatggtt
    45661 tcttccacta tcgctagtgc tgatgcgcaa gaaattgcta ccgctctggc tggattcgat
    45721 ctggaaatgc tggatgcgcc agtttctggt ggtgcagtaa aagccgctaa cggtgaaatg
    45781 actgtcatgg cctccggtag cgatattgcc tttgaacgac tggcacccgt gctggaagcc
    45841 gttgccggaa aagtttatcg cataggtgca gaaccgggac taggttcgac cgtaaaaatt
    45901 attcaccagt tgttagcggg cgtacatatt gctgccggag ccgaagcgat ggcacttgca
    45961 gcccgtgcgg ggatcccgct ggatgtgatg tatgacgtcg tgaccaatgc cgccggaaat
    46021 tcctggatgt tcgaaaaccg gatgcgtcat gtggtggatg gcgattacac cccgcattca
    46081 gccgtcgata tttttgttaa ggatcttggt ctggttgccg atacagccaa agccctgcac
    46141 ttcccgctgc cattggcctc aacagcattg aatatgttca ccagcgccag taacgcaggt
    46201 tacgggaaag aagacgatag cgcagttatc aagattttct ctggcatcac tctaccggga
    46261 gcgaaatcat gatcaagatt ggcgttatcg ccgatgattt taccggcgcg acggatatcg
    46321 ccagttttct ggtggaaaac ggtctaccaa cggtacaaat taacggtgtt ccaacaggta
    46381 aaatgccgga agcaatcgac gcactggtga tcagcctgaa aacgcgctcc tgtccggtgg
    46441 ttgaagccac acagcaatcg ctggcggctc tgagctggtt gcaacagcaa ggttgcaaac
    46501 agatctattt caaatactgc tctactttcg acagtacggc gaaaggtaat atcggcccgg
    46561 ttaccgatgc attaatggat gctctcgaca cgccgtttac ggtcttctct ccggccctgc
    46621 cggtcaacgg acgtacggtt tatcaggggt atttgttcgt aatgaatcaa ctgctggccg
    46681 aatccgggat gcgccatcac ccggtaaatc ccatgaccga cagctatctt ccccgtctgg
    46741 ttgaagcgca atccacaggg cgctgcggcg tcgtttcggc acatgttttc gaacaaggtg
    46801 tggatgccgt tcgtcaagag ctggctcgct tacagcaaga gggctaccgc tacgcggtgc
    46861 ttgatgcgct gaccgaacac catctggaaa ttcagggaga agccttgcgc gatgccccac
    46921 tggtaacggg cggttctggt ctggcgattg gcctggcccg gcagtgggcg caagaaaacg
    46981 gtaaccaggc tcgcgaagca gggcatccgc tcgctgggcg cggcgtagtg ctctccggtt
    47041 catgctctca aatgaccaac cgccaggtgg cacattaccg tcaaattgca ccagcccgtg
    47101 aagttgatct ggcacgctgc ctctcaaccg aaactctggc cgcttatgca cacgaactgg
    47161 cagagtgggt tctgggccag gaaagtgtac ttgctccact ggtttttgcc accgccagca
    47221 ctgacgcatt ggcagcaatt caacagcaat acggtgcaca aaaagccagt caggcagtag
    47281 aaactctatt ttctcaacta gcggcgcggt tagcagcgga aagcgtgaca cgctttattg
    47341 tcgcaggcgg tgagacctcc ggcgtagtca cacagagcct gggcataaaa gggtttcata
    47401 ttggcccaac catttccccc ggcgtgccgt gggtaaacgc actggataag cctgtctcac
    47461 tcgcccttaa atctggcaac ttcggtgatg aagccttttt ttcacgagcc caaagagagt
    47521 ttttatcatg agcgatttcg caaaagtaga gcagtctttg cgagaggaga tgacgcggat
    47581 tgccagttca ttctttcagc gcggctatgc aaccggttcg gctggcaatc tgtcgctgct
    47641 tttacctgac gggaatttac tggcgacacc gacaggttca tgcctgggca atctcgatcc
    47701 gcagcggctt tccaaagtcg ccgcggatgg cgaatggtta agtggtgaca aaccctcgaa
    47761 agaggtgctc tttcatctgg cgctgtatcg caacaatccg cgctgtaaag cggtggtgca
    47821 tttgcacagc acatggtcga cggcgctttc ctgcctgcaa gggctggaca gcagcaacgt
    47881 tattcgtccg ttcacaccat acgtggtgat gcggatggga aatgtcccgc tggtgcctta
    47941 ttaccgaccg ggcgataaac gcatcgcaca ggatctggcg gaactggcag cagacaatca
    48001 ggctttttta ctggcaaatc atggcccagt ggtttgcggt gaaagcctgc aagaagccgc
    48061 caacaatatg gaagagctgg aggaaacggc aaagctgatt tttattctcg gtgaccgccc
    48121 gatccgttat ctgaccgcag gtgaaattgc ggaattaagg agttaatgca atgcctcgtt
    48181 ttgcagctaa tttatccatg atgttcaccg aagtgccttt tattgaacgc ttcgccgcag
    48241 cgcgaaaagc cgggttcgat gctgtggaat ttctgtttcc ctatgactac tccaccctgc
    48301 aaatccaaaa gcaactggag caaaaccatc tgacactggc gctgttcaat accgctcccg
    48361 gagatattaa tgcgggggaa tggggattat ccgcccttcc cggacgtgag cacgaagcac
    48421 acgcagacat cgacctggca ctggaatatg cattggcgct caattgtgaa caagtccatg
    48481 tgatggcagg cgtcgtgccc gctggcgaag acgctgaacg gtaccgggca gtatttatcg
    48541 ataatctccg ctacgctgcc gaccgctttg cgctacacgg caagcgaata ttagttgaag
    48601 cactcagccc cgacgttaag cctcattatc tcttctccag ccagtatcag gcactggcta
    48661 ttgttgaaga ggttgcgcga gataacgtgt ttattcaact agatactttt catgcgcaaa
    48721 aagtagatgg caacctgacg catttaatcc gcgactacgc tgggaaatat gcgcacgtac
    48781 aaattgccgg actacctgat cgacatgaac cggacgatgg agaaatcaac tatccgtggc
    48841 tgttccgcct gttcgatgag gtgggatatc agggctggat cggttgtgaa tataaacctc
    48901 gtggcctcac cgaagaaggg cttggctggt ttgacgcctg gcgctaattc gttaaatccc
    48961 taattacaac gtacccatac atccccccat ctacggatgg ggtaggttaa ctataacttt
    49021 cagacagggt ttcccatgtc cacaattaca ttgttatgca ttgcgttagc tggcgtaatc
    49081 atgctgttgc tgctggtcat caaggcaaag gtacaaccat tcgttgctct gctcctcgtc
    49141 agcctgttag tcgcacttgc ggcgggtata ccggcgggcg aagtgggtaa agtgatgatc
    49201 gccgggatgg gcggtgtgct tggttccgtc actattatta ttggtctggg cgctatgctg
    49261 gggcgtatga tcgaacactc tggtggtgca gagtcactgg ctaattattt cagtcgcaag
    49321 ttaggtgaca aacgaactat cgctgcgctg actctggcag cgttcttcct cggtattccc
    49381 gtcttctttg atgtcggctt tattattctt gcgccaatca tttacggttt tgccaaggtt
    49441 gccaaaatat cgccactcaa atttggcctg cctgtcgctg gaatcatgct aactgttcat
    49501 gtggcggtac cgccgcatcc aggccctgtc gccgcagcgg ggttactcca cgcagacatc
    49561 ggctggctaa ccatcatcgg tattgcgatt tctattcccg tagggattgt tggctacttt
    49621 gcagcgaaaa taatcaatac gcgccaatat gcgatgtcgg tggaagtact ggaacagatg
    49681 caactggctc cggccagtga ggaaggcgca acaaaattaa gcgataaaat aaatccaccg
    49741 ggcgtcgcgc tggtcacctc gctaattgtt attcctatcg cgattatcat ggcgggtacg
    49801 gtttccgcaa cactgatgcc gccttcgcat cccctgcttg gtacgctaca gctgatcggc
    49861 tcaccaatgg tagctctaat gattgcgctg gtgctggcat tctggttatt ggctttacgt
    49921 cgcggctgga gcttacaaca caccagcgac attatgggct cagcccttcc cactgcggcg
    49981 gtagtgattt tggttactgg tgctggaggg gtatttggca aagtgctggt ggaatcgggc
    50041 gtcggcaaag cccttgccaa tatgctacaa atgattgacc tgcctctgtt accagccgca
    50101 tttattattt cactggctct gcgtgcatcg caggggtcag caaccgtagc aatcctgaca
    50161 accggcgggt tactctcaga agcggtgatg ggattgaatc cgattcagtg cgtattggtg
    50221 acgctggcag cctgctttgg tgggcttggc gcgtcacata ttaatgactc agggttctgg
    50281 attgtgacca aatatctggg gttgtcggta gcagacggtc tgaaaacctg gacagtgtta
    50341 acgaccattc tcggttttac cggattctta attacctggt gcgtatgggc ggtaatttga
    50401 ccttaataaa aaggtccgat gggcatcgga ccttttattt aacgcttacc ttccaccagc
    50461 agcggcggga tgctcggcac cattggcgcg tcatcaatat ctttctgcgt catgcggaac
    50521 gcttcgggat aatgttcgcg gctggtacgg cgcagcggtt cggtatcgcg ccaggtataa
    50581 aggcaatgct ggcactgata taccgtccag acatctttca ccggcgattt cgccatcact
    50641 tcaatctgtt catcggcaca acgtggacaa atcatcttct tctccttatt tacgtgcagc
    50701 cagcatagcg gtcagttttt cagcccaggc tttggtttcc ggtaaatcca ccaccggttg
    50761 gctgtagtga ccacggttgt ccggggcgac aggcgtagtg gcgtcgataa tcagcttgtc
    50821 ggtgatcccc gcagggcttg agcctggatc gagttccagc acggacatat tcggcaactg
    50881 caccaaatcc cctgccgggt tcactttcga ggagagcgcc cacatcacct gcggcaggtt
    50941 gaacgggtca acgtcttcat cgaccataat caccatcttc acgtagccca gaccatgcgg
    51001 cgtggtcatt gcgcgcaggc ccaccgcgcg ggcaaagccg ccgtagcgtt ttttggtgga
    51061 gataatcgcc agcaggccat gggtgtacat ggcgtttacc gcctgcactt cagggaactc
    51121 ggctttcagc tgctgataca gcggcacgca ggtggctggc cccatcaggt agtcgatttc
    51181 ggtccacggc ataccgaggt acagcgattc gaaaatcggc ctggtgcggt aagagacttt
    51241 atcgatgcgc accacggtca tgttacgccc gccggagtag tgcccggtaa actcaccgaa
    51301 cggcccttcg atttcgcgtt tacggctttc gatgacccct tcgaggatca cttctgaacc
    51361 ccacggcaca tcaaaaccgg tcaacggggc ggtggcgatc gggtacgggc tttcacgcag
    51421 cgcgcctgcc atttcgtact cggactgatc atatttcagc ggcgtggccc ccatcagcgt
    51481 gatgatcgga tcgttaccga gcgtgatcgc aatcggcaga tcttcaccgc gctcttctgc
    51541 tttatgcaga tgcagggcga tatcgtgcat cggcaccggt tgcaggccga gcttacgctt
    51601 gcccttcact tccatgcggt agatgccgac gttctgcttg ccgaagttat ccgggtcgag
    51661 cggatcgcgg gaaaccacgc acgctttgtc gagatagaaa ccgccatcgt ttaaacgaaa
    51721 cagcggcagg atatcgaaca ggttgatctc gtcgccatca acggtgttct gcgcccaggc
    51781 tggattggcg cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc
    51841 aatctgcttt ttaaccgggg catttggcgg caggccgagg gaaatcgcgt ggttctgcca
    51901 ggaaccgatg gtgttcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca
    51961 cagcgcgggc gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg
    52021 ctcggcgttc acttcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa
    52081 aaagctgcgt aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc
    52141 ggcaatcctt gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg
    52201 gctaccacat ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc
    52261 ggcggcacca tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg
    52321 ctaagcggca tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc
    52381 gccgcgcgcc ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata
    52441 ctgcacggaa taacgatcat gccgtcggta cgaaaagaac cggatgagat gatcgccgcc
    52501 tgatccgccg ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc
    52561 gtttccagtt caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc
    52621 ggcatctccc gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc
    52681 atcccgacga tcagtttcat tgcttatcct cttaattgtt cgtatacgaa cgttattatt
    52741 aacatactcc ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca
    52801 ctaaaaaacc gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa
    52861 taaagcgttc catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga
    52921 gttacctgac ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg
    52981 tatcgaacag gtggcactga tagaagcagc tgtcagcacc aaagcaacgt tagcagaaat
    53041 gttggcaaga atggaaaatc gtggcttagt cagacgtgaa catgaccccg ctgataagcg
    53101 gcgacgcttt gtctggttga ctgccgaagg cgaaaagata cttgctgcgg cgatacccat
    53161 tggtgacagc gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt
    53221 tatgcagctg gtgcgcaaga tgatgaacgc atagggggca aatttccgga tgcgacgcta
    53281 tcgcgtctta tccagactac aaatacaccg actcaccaac cgaaagaaaa aaggccagcc
    53341 tcgcttgaga ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat
    53401 tcagcccctg cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc
    53461 gaacacgttc acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt
    53521 accccagcaa accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca
    53581 gccatttgac gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct
    53641 ctttttcatc ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt
    53701 ctaccgaggt aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat
    53761 ccagttgctc tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac
    53821 gtgcggttcg caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac
    53881 gggtttggtt cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg
    53941 agaagcggaa accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc
    54001 cctcttcgat aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta
    54061 ccaccagacg caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg
    54121 cgcgacgcgc aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac
    54181 caaggtaaag ctgagtcgcg tccaatacac gctgtgtggc tccctgcgat aacagttcct
    54241 cttcggccaa atcgttatca ctgggttcct cttcaactaa ggccttttcg tcaaaaacct
    54301 caactccgtt ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct
    54361 gactcataag gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt
    54421 tccgccgatt tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg
    54481 aatttcaaaa tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg
    54541 ccccgccttc acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact
    54601 caggtaatca tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc
    54661 ggcataaaca acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat
    54721 atcaatcccc ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt
    54781 cggccagcgc caggtggaga taggcgtact ggttgatgta ctgctgacag ttggctcggt
    54841 tgtgcttgct gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagctg gcttgttgtt
    54901 cggcaacatt ttgttagcac tctgttcacc cgaagattca gaatacgtaa ttgtcggttg
    54961 cgacgcaaca gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc
    55021 gtcggcccgg gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg
    55081 caaggtctga ccaacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc
    55141 acggaaatcg ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt
    55201 ataggtactg ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat
    55261 gcgtccgttt tccatctgta ccggctgctg agctactggc tgcactggct ggatttgcgg
    55321 ttgttgagta gcctgaattt gtggctgctg taccggctga atttgcggtt gctgggctgt
    55381 agacgtcgtc cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg
    55441 cgcattgcca ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc
    55501 cagccatagc gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc
    55561 cgcgctcatt tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa
    55621 ggcaccctac tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc
    55681 aggaaaaatc caggtatttc ctcacgtttt aagccagctc acccttcact aaaggtacaa
    55741 agcgcacggc ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca
    55801 aatactggtg ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca
    55861 ttagcgcagt tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcgc
    55921 gtgcctgcca accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat
    55981 ttttcaggcg gcgacgcgcc tgccactgca aacctttaat ccgttcgacc gagcaaacat
    56041 gctggacaag atgcgccagg attgccgttt gatatcccga accagtgcca atttccagca
    56101 cccgcgactg cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg
    56161 aaattgtctg cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg
    56221 cttcatcaac gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct
    56281 gaataccttg cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt
    56341 gccaactccc acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa
    56401 atccacatgc agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc
    56461 cggaccggca tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg
    56521 atcttgctgc gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat
    56581 acctttgatt tgatccaagg gtaaatccgg aacgttaata ttaagaatac gcccggtgcg
    56641 caacggctct ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta
    56701 atgtttatgc ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat
    56761 cgcggcggct accgtaccgg aataaataac atcatccccc agattcggcc cggcgttaat
    56821 tccggacaca acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca
    56881 atcggtcggg gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag
    56941 ggaggattcc agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac
    57001 ctgaacgtca gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac
    57061 cccgtcatca ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga
    57121 caacgctggt tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat
    57181 cccaccaatt ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa
    57241 ctttttcgcg caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gcttgttcga
    57301 atgccagcgc ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca
    57361 tcagctcttt atcgttaacg cgacgctgta attccgccag ttcttcggtg gttgcgacaa
    57421 accagctacc acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg
    57481 cttttttgag gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc
    57541 aaaaactccg tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgctgcgccc
    57601 cctgcaagtt gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt
    57661 ttacgcaaat atcgttcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa
    57721 ccagggtaaa ggcgtttcct ttcagcgccc ctaaacgcag cttgcgcttg tgccgcgcat
    57781 actccagcac ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcccg
    57841 gcacgcgagc gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc
    57901 tgacttcacg ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat
    57961 tgcagccgtt tttgaggatt ctaaccagaa tatactcacc ttcaccatca ggctcaaagc
    58021 ccaaatcttc caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg
    58081 gtttaccgtg gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag
    58141 cgccaccgct tcacaggcaa tcccttcccc acgtccggta aatcccagtt tttccgtagt
    58201 agtggctttc acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg
    58261 catttgtgga atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt
    58321 gccaagggta taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct
    58381 atcggcacct ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc
    58441 cgccgcgcca agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc
    58501 cagcaatcct ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc
    58561 aaaggcatgt acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt
    58621 cgggtgaggt aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc
    58681 gcacggcctt cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg
    58741 gtaatagtcg cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg
    58801 aaaaattgcg gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca
    58861 tttttgcccg gttcggcacg tttcatagta tcgcgcactg gtgcggcgag gatccccccc
    58921 gtgcggctgg tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg caaacaagga
    58981 cgagcggcgt catgcaccaa tacccactgc gcgtcgccag cggctttcag acctgccagc
    59041 acggaatcgg cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga
    59101 ggaagttgtg caaaacggct atcgccagga cttatggcaa tgacgactcg tttcacccgg
    59161 ggatgcgcca gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga
    59221 tattgcttag gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg
    59281 caaacatcca aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc
    59341 cccgcagact gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc
    59401 ctggtcatgc tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga
    59461 tcgtcaattt cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc
    59521 tgtgccgcca catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac
    59581 agcgaatact gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg
    59641 ccccctgaaa aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg
    59701 gggataccgc gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg
    59761 gcgtgcagaa cataatctca ttattagtta cggtttgaat tatgaacaga ggagacggga
    59821 aagtacaaat tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac
    59881 cagggtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc
    59941 caccactacc gcgacgggca tcagcgccag aaagaaaggc caggtgtaga taaagaagaa
    60001 cagcgtgtta gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa
    60061 accgaccacg gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt
    60121 atttgttagt gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg
    60181 ggcatggaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta
    60241 ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt
    60301 caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt
    60361 tcttatataa gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca
    60421 cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg
    60481 gcgagataaa tgcggtcagc actaccagtc cggcttcaac catcaaattc gccacttcac
    60541 cgacgcgacg gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc
    60601 cgtggcgaac attatcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct
    60661 cctccagcgc cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca
    60721 ctacaccacg atgaccgtgg tgtagctcac gttgttgcac agtgaccgga tggctatgcc
    60781 agacgacgtt ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca
    60841 gtgcggaaag tggcgacgaa ccagagcatt caattccagt tcgaatgcac tgaattcaga
    60901 tggcgcagca gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt
    60961 gctcaggcga tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat aacgatctaa
    61021 caccagcggc tcgtcaaaag tgagatccac gaggccgatc ccattcagtg gcaggttttc
    61081 aacttcacgc tgggtaaggt tattaatatc aacctgatag cgaatgccat caacacgcgc
    61141 gcgcgtcttc ttaccggcaa ttttgatgtc gtaactctgc cctggagaaa gcggctgttc
    61201 cgccatccat accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt cgtctgccgc
    61261 cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc
    61321 tccggcaaag gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc
    61381 cacaccagag ggcagcactt tgacacgttg cccgacttcc acgcgaccgg atgccagcgt
    61441 tccggcgtaa ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat
    61501 tggctgagca tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg
    61561 accgctgtac cacggcatac tttcactttg cgatgccacg ttgtcgcctt ccagtgcaga
    61621 gagcggcaca aagcggatat ccagattacc cggcagctgc ccggcaaagg tcaaataatc
    61681 ttcacgaata cgggtgaacg tctcttcact gtaatccacc agatccattt tgttgatcgc
    61741 cacgaccaga tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc
    61801 gagcacgcct ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg atgcgccagt
    61861 cgccatattg cgggtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt
    61921 ctcggtagag aaatagcggt aggccacgtc aatggtgatg ccctgttcgc gctcagcttg
    61981 caggccgtcc accagcagag ccagatccag cttttcgccc tgggtgccgt gacgcttact
    62041 gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcagacg
    62101 accaatcaga gtacttttgc cgtcatcgac gctaccactg gtcagaaaac gcagcaggct
    62161 tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc
    62221 aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct
    62281 ggtcgcggtc aatcacgcgg ccctgacgtt cactggtggt ggaaaccagc atctcttcga
    62341 tgatttccgg cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca
    62401 gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt
    62461 tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat
    62521 atagcggaac aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc
    62581 agttagagag cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt
    62641 gccacagctc cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt
    62701 aaattcgctc tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat
    62761 caaaaccgta tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt
    62821 tcgcgctgcc gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca
    62881 ccagcagttc gcagccgtag gctttagcag tacgatcgcg gaactcatac atctcgcgga
    62941 atttccagcc ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct
    63001 tgcgcgccag atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat
    63061 ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca
    63121 ggtgagtaag tcgtatttga tccataaccg ttcctttgca ataccgctat tttcttgcca
    63181 tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt
    63241 agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg
    63301 atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg
    63361 cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt
    63421 accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa
    63481 cctggcgatt ttgctaatac tcaggcacga catattgcta ctttctttcc gggacgcatg
    63541 accggaactc ctgcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg
    63601 ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat
    63661 cgcaagagct ggcataacgt gacgggaagt acggtgattg ccgctcatga aggcaaagcg
    63721 ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac
    63781 gccgatgcca atctcggcgg gcttacgtta caaggaatgg atgataacgc cgcaggttta
    63841 ggtgtcatgc tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga
    63901 tttgtggcga ccagcggcga agaggaaggg aaattaggcg cagagaattt actcaagcgg
    63961 atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt
    64021 ggcgataaat tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg
    64081 cgcgacaggg cgctggcaat tgcgcgtagt catggaattg ccgcaacgac caatccgggt
    64141 ttgaataaaa attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa
    64201 gcgggcattg ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt
    64261 tatcagcaac gcgcaaaaac acctgccttc ccggcgggaa atagctggca tgacgtaaga
    64321 ctggataatc aacaacatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt
    64381 gatgttatgc ggataatgct accgctggtg aaggagctgg cgaaggcgtc ttgatgggtt
    64441 tgaaaatggg agctgggagt tctaccgcag gggcggggaa ttctaagtga tatccatcat
    64501 cgcatccagt gcgcccggtt tatccccgct gacgcgggga acacaaaacc aaacttctcc
    64561 ataaattcca tagccgcggt ttatccccgc tggcgcgggg aacacgagag tgctgacagg
    64621 tgtctcgatt acctgatcgg tttatccccg ctggcgcggg gaacacgaac gcaatatcac
    64681 ggcgttctgc ggcctcggcg gtttatcccc gctggcgcgg ggaacacgtc gggccgggta
    64741 agtgaacctg tcggt
//
LOCUS       DAZGTR010000013        64516 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGTR010000013 DAZGTR010000000
VERSION     DAZGTR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64516
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.013"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            6..722
                     /locus_tag="ACHLCY_000959"
     CDS             6..722
                     /locus_tag="ACHLCY_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0368270.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            752..1252
                     /locus_tag="ACHLCY_000960"
     CDS             752..1252
                     /locus_tag="ACHLCY_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFP0368271.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1344..4028
                     /locus_tag="ACHLCY_000961"
     CDS             1344..4028
                     /locus_tag="ACHLCY_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFP0368272.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADISDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDGLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQRAVAP"
     gene            4025..5113
                     /locus_tag="ACHLCY_000962"
     CDS             4025..5113
                     /locus_tag="ACHLCY_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFP0368273.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLVVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5201..6499)
                     /gene="exuT"
                     /locus_tag="ACHLCY_000963"
     CDS             complement(5201..6499)
                     /gene="exuT"
                     /locus_tag="ACHLCY_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP0368274.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6982..8394
                     /gene="uxaC"
                     /locus_tag="ACHLCY_000964"
     CDS             6982..8394
                     /gene="uxaC"
                     /locus_tag="ACHLCY_000964"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP0368275.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8409..9896
                     /gene="uxaA"
                     /locus_tag="ACHLCY_000965"
     CDS             8409..9896
                     /gene="uxaA"
                     /locus_tag="ACHLCY_000965"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP0368276.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9979..10530
                     /gene="ygjV"
                     /locus_tag="ACHLCY_000966"
     CDS             9979..10530
                     /gene="ygjV"
                     /locus_tag="ACHLCY_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP0368277.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10535..11779)
                     /gene="sstT"
                     /locus_tag="ACHLCY_000967"
     CDS             complement(10535..11779)
                     /gene="sstT"
                     /locus_tag="ACHLCY_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP0368278.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12178..13143)
                     /gene="alx"
                     /locus_tag="ACHLCY_000968"
     CDS             complement(12178..13143)
                     /gene="alx"
                     /locus_tag="ACHLCY_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP0368279.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13426..14412)
                     /gene="ygjR"
                     /locus_tag="ACHLCY_000969"
     CDS             complement(13426..14412)
                     /gene="ygjR"
                     /locus_tag="ACHLCY_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0368280.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14491..15174)
                     /gene="ygjQ"
                     /locus_tag="ACHLCY_000970"
     CDS             complement(14491..15174)
                     /gene="ygjQ"
                     /locus_tag="ACHLCY_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP0368281.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15251..15754)
                     /gene="ygjP"
                     /locus_tag="ACHLCY_000971"
     CDS             complement(15251..15754)
                     /gene="ygjP"
                     /locus_tag="ACHLCY_000971"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP0368282.1"
                     /translation="MSNLTYLQGYPELLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTPDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15839..16975
                     /gene="rlmG"
                     /locus_tag="ACHLCY_000972"
     CDS             15839..16975
                     /gene="rlmG"
                     /locus_tag="ACHLCY_000972"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP0368283.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSTGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17166..17249
                     /locus_tag="ACHLCY_000973"
                     /pseudo
     CDS             17166..17249
                     /locus_tag="ACHLCY_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17246..17662
                     /gene="higA"
                     /locus_tag="ACHLCY_000974"
     CDS             17246..17662
                     /gene="higA"
                     /locus_tag="ACHLCY_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP0368284.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(17707..19725)
                     /gene="fadH"
                     /locus_tag="ACHLCY_000975"
     CDS             complement(17707..19725)
                     /gene="fadH"
                     /locus_tag="ACHLCY_000975"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP0368285.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHISLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAIELDARRAIAQGTRLALEI"
     gene            complement(20051..22402)
                     /gene="ygjK"
                     /locus_tag="ACHLCY_000976"
     CDS             complement(20051..22402)
                     /gene="ygjK"
                     /locus_tag="ACHLCY_000976"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP0368286.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22419..23489)
                     /gene="ygjJ"
                     /locus_tag="ACHLCY_000977"
     CDS             complement(22419..23489)
                     /gene="ygjJ"
                     /locus_tag="ACHLCY_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP0368287.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(23623..25056)
                     /gene="ygjI"
                     /locus_tag="ACHLCY_000978"
     CDS             complement(23623..25056)
                     /gene="ygjI"
                     /locus_tag="ACHLCY_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0368288.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTILSMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25119..25568)
                     /gene="ebgC"
                     /locus_tag="ACHLCY_000979"
     CDS             complement(25119..25568)
                     /gene="ebgC"
                     /locus_tag="ACHLCY_000979"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP0368289.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25565..28657)
                     /gene="ebgA"
                     /locus_tag="ACHLCY_000980"
     CDS             complement(25565..28657)
                     /gene="ebgA"
                     /locus_tag="ACHLCY_000980"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP0368290.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQENEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYELGAGFFSTNLHSENKQ
                     "
     gene            complement(28841..29824)
                     /gene="ebgR"
                     /locus_tag="ACHLCY_000981"
     CDS             complement(28841..29824)
                     /gene="ebgR"
                     /locus_tag="ACHLCY_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP0368291.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30043..30375
                     /gene="ygjH"
                     /locus_tag="ACHLCY_000982"
     CDS             30043..30375
                     /gene="ygjH"
                     /locus_tag="ACHLCY_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP0368292.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(30417..31796)
                     /gene="ygjG"
                     /locus_tag="ACHLCY_000983"
     CDS             complement(30417..31796)
                     /gene="ygjG"
                     /locus_tag="ACHLCY_000983"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP0368293.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            32214..33734
                     /gene="aer"
                     /locus_tag="ACHLCY_000984"
     CDS             32214..33734
                     /gene="aer"
                     /locus_tag="ACHLCY_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP0368294.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VCEGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(33888..34511)
                     /gene="nfeR"
                     /locus_tag="ACHLCY_000985"
                     /gene_synonym="yqjI"
     CDS             complement(33888..34511)
                     /gene="nfeR"
                     /locus_tag="ACHLCY_000985"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP0368295.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            34788..35552
                     /gene="nfeF"
                     /locus_tag="ACHLCY_000986"
                     /gene_synonym="yqjH"
     CDS             34788..35552
                     /gene="nfeF"
                     /locus_tag="ACHLCY_000986"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP0368296.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPLSRDYTPLYDELRHELAID
                     FFIHDGGVSSGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(35606..35681)
                     /locus_tag="ACHLCY_000987"
     tRNA            complement(35606..35681)
                     /locus_tag="ACHLCY_000987"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35646..35648),aa:Ile,seq:cat)
     gene            35806..36312
                     /gene="mug"
                     /locus_tag="ACHLCY_000988"
     CDS             35806..36312
                     /gene="mug"
                     /locus_tag="ACHLCY_000988"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP0368297.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            complement(36391..38232)
                     /gene="rpoD"
                     /locus_tag="ACHLCY_000989"
     CDS             complement(36391..38232)
                     /gene="rpoD"
                     /locus_tag="ACHLCY_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP0368298.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(38427..40172)
                     /gene="dnaG"
                     /locus_tag="ACHLCY_000990"
     CDS             complement(38427..40172)
                     /gene="dnaG"
                     /locus_tag="ACHLCY_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0368299.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNLELAKK"
     gene            complement(40283..40498)
                     /gene="rpsU"
                     /locus_tag="ACHLCY_000991"
     CDS             complement(40283..40498)
                     /gene="rpsU"
                     /locus_tag="ACHLCY_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP0368300.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            40736..41749
                     /gene="tsaD"
                     /locus_tag="ACHLCY_000992"
     CDS             40736..41749
                     /gene="tsaD"
                     /locus_tag="ACHLCY_000992"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP0368301.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(41792..43255)
                     /gene="ttdT"
                     /locus_tag="ACHLCY_000993"
     CDS             complement(41792..43255)
                     /gene="ttdT"
                     /locus_tag="ACHLCY_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP0368302.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(43303..43908)
                     /gene="ttdB"
                     /locus_tag="ACHLCY_000994"
     CDS             complement(43303..43908)
                     /gene="ttdB"
                     /locus_tag="ACHLCY_000994"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP0368303.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(43905..44816)
                     /gene="ttdA"
                     /locus_tag="ACHLCY_000995"
     CDS             complement(43905..44816)
                     /gene="ttdA"
                     /locus_tag="ACHLCY_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP0368304.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            45023..45955
                     /gene="ttdR"
                     /locus_tag="ACHLCY_000996"
                     /gene_synonym="dan"
     CDS             45023..45955
                     /gene="ttdR"
                     /locus_tag="ACHLCY_000996"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP0368305.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQHNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDKGYQVM"
     gene            complement(45968..46585)
                     /gene="plsY"
                     /locus_tag="ACHLCY_000997"
     CDS             complement(45968..46585)
                     /gene="plsY"
                     /locus_tag="ACHLCY_000997"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP0368306.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            46690..47058
                     /gene="folB"
                     /locus_tag="ACHLCY_000998"
     CDS             46690..47058
                     /gene="folB"
                     /locus_tag="ACHLCY_000998"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP0368307.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            47148..47969
                     /gene="bacA"
                     /locus_tag="ACHLCY_000999"
     CDS             47148..47969
                     /gene="bacA"
                     /locus_tag="ACHLCY_000999"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0368308.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(48126..49364)
                     /gene="cca"
                     /locus_tag="ACHLCY_001000"
     CDS             complement(48126..49364)
                     /gene="cca"
                     /locus_tag="ACHLCY_001000"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP0368309.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPLTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(49428..50048)
                     /gene="ygiM"
                     /locus_tag="ACHLCY_001001"
     CDS             complement(49428..50048)
                     /gene="ygiM"
                     /locus_tag="ACHLCY_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP0368310.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            50290..51591
                     /gene="ygiF"
                     /locus_tag="ACHLCY_001002"
     CDS             50290..51591
                     /gene="ygiF"
                     /locus_tag="ACHLCY_001002"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP0368311.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            51614..54454
                     /gene="glnE"
                     /locus_tag="ACHLCY_001003"
     CDS             51614..54454
                     /gene="glnE"
                     /locus_tag="ACHLCY_001003"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP0368312.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            54502..55935
                     /gene="hldE"
                     /locus_tag="ACHLCY_001004"
     CDS             54502..55935
                     /gene="hldE"
                     /locus_tag="ACHLCY_001004"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP0368313.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKDALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(56055..56114)
                     /gene="ibsE"
                     /locus_tag="ACHLCY_001005"
     CDS             complement(56055..56114)
                     /gene="ibsE"
                     /locus_tag="ACHLCY_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP0368314.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(56431..56490)
                     /locus_tag="ACHLCY_001006"
     CDS             complement(56431..56490)
                     /locus_tag="ACHLCY_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0368315.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            complement(56729..58390)
                     /gene="yqiK"
                     /locus_tag="ACHLCY_001007"
     CDS             complement(56729..58390)
                     /gene="yqiK"
                     /locus_tag="ACHLCY_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0368316.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            complement(58417..59046)
                     /gene="yqiJ"
                     /locus_tag="ACHLCY_001008"
     CDS             complement(58417..59046)
                     /gene="yqiJ"
                     /locus_tag="ACHLCY_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0368317.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            59315..59515
                     /gene="glgS"
                     /locus_tag="ACHLCY_001009"
     CDS             59315..59515
                     /gene="glgS"
                     /locus_tag="ACHLCY_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP0368318.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            complement(59558..60622)
                     /gene="yqiI"
                     /locus_tag="ACHLCY_001010"
     CDS             complement(59558..60622)
                     /gene="yqiI"
                     /locus_tag="ACHLCY_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0368319.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNQYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(60624..61372)
                     /gene="yqiH"
                     /locus_tag="ACHLCY_001011"
                     /pseudo
     CDS             complement(60624..61372)
                     /gene="yqiH"
                     /locus_tag="ACHLCY_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
     gene            complement(61388..63892)
                     /locus_tag="ACHLCY_001012"
     CDS             complement(61388..63892)
                     /locus_tag="ACHLCY_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0368320.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGCFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            complement(63952..64503)
                     /gene="ygiL"
                     /locus_tag="ACHLCY_001013"
     CDS             complement(63952..64503)
                     /gene="ygiL"
                     /locus_tag="ACHLCY_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0368321.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
BASE COUNT        15713 a        16138 c        16859 g        15806 t
ORIGIN      
        1 tttatatggt ggcgaaaatt aatctcttat cacggcttac gccgctttta ttcgtgttcg
       61 ctcctttttt ggcacagtca aatatgactg tttatcctat ggctgtttcc attaatagtc
      121 agggtgaagg caacgttcgc gtaatatcta aaagtaatga agttcagtac atcaaggcga
      181 cagtattccg tatcgataat ccatcgacgc ctcaggaaaa tgaagttgag attaaatccg
      241 gtgatgcaaa tcatctggtt gttatgccac ctaaattcgc tttaccagcc ggtagtagca
      301 aaaccgtacg ttttgttgcg atggaaccag agcaaaaaga gaaaaattat cgcgttaaat
      361 ttgaagcggt tcccagtatt gatgacgttg ccacagataa aaaagatctc tctatgcagt
      421 taacagttaa cttaatttgg gggattgttg ttagtgttcc acctcagcaa cctattgcta
      481 agttagaagt aaatgctgcc caaaaattag ttaatgcagg gaatcaacgc ttaaagattt
      541 taacgatcgc ttattgtaaa aataatagca aagaaaattg taagatacag accgtaaata
      601 aaaacatctt ccctggtcag gaaagaaatc ttgaaagcat atctggctac gacaaaatcg
      661 ttgtcaaata taataactgg atcaccaaag ataatggcga gtttgaactg gcggtccatt
      721 aattatcatt gtaataaacg aaggaatagt aatgaaaaag gtatttgcaa aatctcttct
      781 ggtcgcagcg atgttttctg ttgcaggctc cgcattggca gtacaaaagg atattactgt
      841 aacggccaac gttgatgccg ctctggatat gacacagacc gataacaccg cgttgccgaa
      901 agcagtagaa atgcaatatc tgccggggca gggtctgcaa tcttaccagt tgatgaccaa
      961 aatctggtct aacgacgtta ccaaagatgt aaaaatgcag ttagtttccc cggcgcaact
     1021 ggttcaaagc ctggatgcta gcaagattgt tccgctgaca gtaacctggg gcggtgaaga
     1081 aattaaagct gatgcggcaa ccacttttac cgcaaccaaa atctttgctt ccgatgcgtt
     1141 aactaacggt tctctggcta aaaacctgat gtttgctcaa accaccaaag gtgttctgga
     1201 gacaggcatc taccgtggtg tagtaagcat ttatctgtct caggatatct aatccagata
     1261 acagaattaa cacgtaacaa agggaggggt agccctccct tattttaact gttttagtat
     1321 ttgttatgga tttcaggatt gccatggata aaaaattact ggctcttttg atcctggcga
     1381 gtctcagccc ggcagaggcg acattaacca aaattcccgc agggtttgag gttattgctc
     1441 agggacagca ggagtatatc gaggtttatt tttcagggaa aagtctcggt aaatattatg
     1501 caatggttaa tcttgatacc gtaacatttc ttgatccagc aagtctatat aacaagctgg
     1561 aactggatgt agacgatcag aaaatcgcgc atatagtgaa agaaaaatta tcgcagccgc
     1621 tagctcgcca cggtgaattg gcttgcggtt atgtacgtac tgactcaggg tgtggttttc
     1681 tgaataccga tacgctggaa ataatctata atgatgaaga aagttcggca acgttgttta
     1741 ttaatccgca atggaattca gctttcgatg cgaagtcatt atatttaaat ccagacaaaa
     1801 atactgttaa tgcttttata catcagcaag acatcaatgt tctggcacag gatgattacc
     1861 aatcgttgtc tattcaggga aacggtgcgc tgggaataac agaaaatagt tatattggtg
     1921 cacactggaa tttcaacggt tatgatgcag atgatgtcag tgacagtaat gctgacatca
     1981 gcgatctcta ttatcgttat gattttttac gtcgttatta tgtgcaggcg ggtcgcatgg
     2041 acaaccgcac actatttaat gcacaaggcg ggaactttac ctttaacttt ctgccactcg
     2101 gtgcaattga tgggatgcgt atcgggtcga cactgagcta tttaaaccag gcgcaaagcc
     2161 agcaaggaac cccagtaatg gttctgcttt cgcgcaattc tcgtgttgac gcttatcgta
     2221 atgagcaact tttgggatcg ttttatctca atagtggttc gcaatttatt gataccagtt
     2281 cctttccgcc aggcagctac agcgtggcat taaaagtcta cgaaaataac caactcaccc
     2341 gcaccgagct tgtgccgttt accaaaaccg gcggtctgac tgacggaaat gcgcaatggt
     2401 tcttacaggc aggtaaaact acatcacagg tttctgatga tgaaagttca gcttatcagc
     2461 taggagtacg cctgccatta catccgcaat atgagctcta cgcagggctg gcgaatgccg
     2521 atgatgtgag tgctttcgag ttaggtaata actggacggc agatttaggc ggggtgggga
     2581 atcttgcaat cagcgccagc gtgttccgta acgatgacgg cggcaaaggt gatatgcaac
     2641 aggccaactg gagtaatccg ggatggccga cgttgggctt ttatcggacc aactctgacg
     2701 gcgatgcttg tacaaccgac agcagagaga gctataacgc cttaagctgt tatgaaagta
     2761 tttccgcgac ggtttcacag aattttgtcg gctggaatat gatgctgggt tatacccgca
     2821 cacaaaataa cactgatgat agtttgcgtt gggataaaca gcagagcttt gaaaataact
     2881 atcttcgcca gacaacagcg caaagtatct ctgaaactgt acaacttagc gcttcccgcg
     2941 cttttgtgat gcgtgactgg attttgagta cgtcagtcgg tgttttccat cgtaatgaca
     3001 acggtggcga taacgacgac aacggcttgt acttatcgtt ttcgttatct gacacgccaa
     3061 cgatggatag caataacaac agccattcaa caaatgtgtc tacggattat cgttatagcg
     3121 aacaggatgg cgatcaaacg tcatggcagt tatcgcatac cttttataac gattcattca
     3181 gccataaaga acttggcgta accgtcggag gcctgaacac cgataccata aacagcgcgg
     3241 ttaacgggcg ttgggatggg caatacggaa atgtctacgc taccgtatct gatagttatg
     3301 accgtaagaa tcatgatcat ctttcggcct ttacgggcac ttatagctct acgctggctg
     3361 tcagtcgcta tggcgttaat ttgggagcca gtggtacaga cggtttgctg ggtgcggtac
     3421 tggtggatgt gaaaggcttc tctgaacagg atgaagagag tcaggatctg caactcgaag
     3481 cgcgggtggc aggcagccga actttgcagc ttggtcaaag tgacagtgtg ttgttccctt
     3541 atcctggatt tcagtctggt tttgttgagg ttaacgacag tagccagggc aatcagcagg
     3601 ggacaacaaa catcattaac ggtgcaggga atcgtgaatt aatgttgttg cctggcaagc
     3661 tgcgctatcg cgaagtgtct gccagcttta actacaacta tatcggtcgt ttattactgc
     3721 cggcagcggt gaaaaaattc ccgattgtgg ggctgaacag cgccatgtta ctggtagctg
     3781 aagatggcgg atttacactt gaaattaacg gcagcgaaaa agagctctat ctgctttccg
     3841 ggcagcaatt ccttaagtgt ccactgagtg ttgtaaagaa acgcgccagc attcgttaca
     3901 gcggagatgt tacttgtagt gtggtgactt attcacaatt accggagtcg attcaggttc
     3961 aggcacagtt gaaacagcct aaattacgtg gaaacgttca gacggcgcaa agggcggttg
     4021 caccatgaga aatcgattga ttgcggcgat attgggcttg tttggcacgc tcactggcgt
     4081 tcaggcagct cctgacgtga ccagtgaaat tacgtatgat ttggcatctg gtagagcgga
     4141 ttattacttc tggaaggatg aagcgtcggc aggaaataat ggatatatgt ggtatgaatg
     4201 ttcgtatcct gacctccaac aaacctgtac agctaatgga aatatatcga cagtacaaat
     4261 ctatttaact gaacaacgca gtgggatgcg ttggccggta aaactcaaag gatttaaaac
     4321 agccattgta agtagcgatg aagcgccgcc aggatgcaag gggggcaaag ggcttcagac
     4381 gaatcttaag gattctaata gatcttcatg tacagaagat ggtcaacatt attatatata
     4441 cgatacaaag tttcttacgc tctaccttga gcagacagag atgaagaatt tgccgattgg
     4501 tggcgtctgg aaggggaaag ttaaattaca ttcgaacagc ccggcccagg actatttcgc
     4561 aaatattacc ctgaatacgc tcgaccccaa ccatattgac gtgttcttcc cggagttcgc
     4621 ccacgccacg ccaagggtgc agttagactt gcatccaaca ggaagcgtta acggcagcaa
     4681 ctacgcgcaa gatctgacca tgttggacat gtgcctgtac gatggtttta acggtaatgc
     4741 catcagttat gaaatcatgc tcaaagatga agggcgacca gctgcagggc gcagagacgg
     4801 ttacttctct atctatcgtc agggagggac caccaccgac gagggagaac gcattgatta
     4861 ccgggtcaaa atgtacaacc cggaaaccgg tgggcaaatt gatgtgcgca ataatgaaaa
     4921 tatggtctgg aacagcatta acctgaaacg tgtgcgtccg gtggtactgc ccggtattcg
     4981 ctatgccgtg atgtgtgtgc cgacaccctt gactctggta gtcgataaat tcagcgtgat
     5041 ggataaacag gccgggtact acatgggcaa attgtcagta atctttacgc cttccttgcc
     5101 aaccatcaat taaccctgca aaccggacaa acgctttttt ctatataaac gacgaagccg
     5161 tctgataaag cggcttcgtc attacaactt aagttaaacg ttaatgttgc ggtgcgggat
     5221 cgttatgtgt ttcctgcgcc acctcaatcg ccggtttgtt ctgcaacacg gtccagataa
     5281 ccagcgcacc taacaggtcg aacactgcca gaactgcgaa cagcgggctg aagccgatgg
     5341 tgtcagccag tgcaccgaca accagcgcaa acagcgtact tgccagccat gcggacatcc
     5401 cggttaaacc gttcgccgtc gccacttcgt tacgaccgaa cacgtcagaa gagagcgtaa
     5461 tcagcgcgcc agacagtgcc tggtgggcaa aaccaccgat acacagcagc ataattgcga
     5521 catacgggtt ggtgaacaga ccgatcatac ccgggccaat catcagcaca gcacccagcg
     5581 ttacgaccat cttacgggaa acgatcaggt tcacaccaaa ccaacgctgg aacagcggcg
     5641 gcaggtaacc accgaggata caaccgaggt cagcaaacag catcggcatc caggcgaaca
     5701 tcgcgatctc tttcaggtta aagccgtaaa ctttaaacat gaacagcggg atccacgcgt
     5761 taaaagtacc ccaggccggt tctgccagga aacgcggcag cgcgataccc cagaactgac
     5821 ggttacgcag gatctgacca acggacattt tcttcgccgt gctcacctgg tgctgggctt
     5881 cctgaccatt aataatatag tcgcgttctt catcggtcag atgcttctgg tcgcgcggat
     5941 gtttatagaa aatcagccat gccatcgccc agataaagct caatgcaccg gagatgataa
     6001 atgccatctg ccagctgtgc attacgattg cccataccac cagcggcggc gcaatcatcg
     6061 caccaatcga agaacctacg ttaaagtaac ctaccgcgat ggaacgctct ttcgccggga
     6121 accattcgga gctggctttc agacccgccg ggatcatcgc tgcttccgcg gcaccgactg
     6181 caccacgagc aatcgccagg ccaccccagc taccagccag cgcggttgca ccacagaaca
     6241 cggcccacag cacagcaaac attgcataac cgattttcgt acccagcaca tccagtacat
     6301 aacctgctac cggttgcatg accgtataag cagcagaata ggctgcgatg atataggaat
     6361 actgttgggt ggagatgttt aactcttcca tcagagttgg cgcagctgcc gccacagtgt
     6421 tacgcgtcag gtaaccaagc acggtgccga gcgtcaccag tgcgatcata taccaacgta
     6481 accctttaat tttacgcatg taaaacctca tcgttttgtt atatccgcag gaagcgcggc
     6541 catcatcccg ccagaggctg aagatgttag ttacgaccgg gtaaccgaaa aaaaaccggc
     6601 acgccccgaa cgttgccatg aaagtttcgt gctaattcgg cttccgtacc ggttaacccg
     6661 attcatctaa caaatgaatg aatcggcaat ttgcattccg tcgacttatg atttgcgacg
     6721 gcagaaagat aacttgtcat acaactttaa aaggtgagag ccatcacaaa tgtgcgaata
     6781 tttgtaggga cattacctga cgacagcgag gccagtactg gcgcggcctg cagagagatt
     6841 taccactttg agagtaattt ttttaactac gtttattgat ctaactcacg aaaatatctt
     6901 cggactctgg aaattggtgt gataactttg tcagcatcgc accataagca agctagctca
     6961 ctcgttgaga ggaagacgaa aatgactccg tttatgactg aagatttcct gttagatacc
     7021 gaatttgccc gccgtctgta tcacgactac gcaaaagacc agccgatttt cgattaccat
     7081 tgccatttgc cgccgcagca gattgcggaa gactatcgtt ttaaaaacct gtatgacatc
     7141 tggctgaaag gcgatcacta caaatggcgc gctatgcgta ccaacggtgt ggccgagcgt
     7201 ctgtgtaccg gtgatgcgtc tgaccgtgaa aaatttgacg cctgggcggc gactgttccg
     7261 cacactatcg gcaacccgtt ataccactgg acgcacctcg aactgcgtcg tccgtttggt
     7321 atcactggca aattgctttc tccgtcaact gccgatgaaa tctggaacga atgtaacgaa
     7381 ctgctggcgc aggataactt ctccgcgcgc ggcatcatgc agcagatgaa cgtgaaaatg
     7441 gtcggcacca ccgatgaccc gatcgattct ctggagcatc acgcagagat cgccaaagac
     7501 ggctctttca ccatcaaagt gctgccgagc tggcgtccgg acaaagcctt caacatcgaa
     7561 caggcgacct ttaacgacta catggcgaag ctgggcgaag tttccgatac cgacattcgc
     7621 cgctttgctg acctgcaaac tgccttgacc aaacgtctgg atcacttcgc cgctcacggc
     7681 tgtaaagtgt ctgaccatgc gctggacgtg gtgatgtttg ctgaagcgaa cgaagcggaa
     7741 ctggacagca ttctggcgcg ccgtctggct ggcgaaaccc tgagcgagca cgaagtggca
     7801 cagttcaaaa ctgcggtact ggtgttcctt ggtgccgaat atgcacgtcg cggctgggta
     7861 cagcagtacc acattggcgc gctgcgtaat aacaacctgc gtcagttcaa actgctgggg
     7921 ccggatgtag gctttgactc catcaacgac cgtccgatgg cagaagagtt gtctaagctg
     7981 ctgagcaagc agaacgaaga aaacctgctg ccgaaaacca ttctgtactg cctgaacccg
     8041 cgcgataacg aagtgctggg caccatgatc ggtaacttcc agggcgaagg tatgccgggc
     8101 aaaatgcagt tcggttccgg ctggtggttt aacgatcaga aagacggtat ggaacgtcag
     8161 atgacccaac tggcgcagct cggtttgctg agccgctttg tcggtatgct gactgacagc
     8221 cgtagcttcc tgtcatacac ccgtcacgaa tacttccgcc gcattctgtg ccagatgatc
     8281 ggtcgctggg tggaagcggg cgaagcaccg gcggacatca acttgctggg cgagatggtg
     8341 aaaaatattt gctttaacaa tgcgcgtgac tacttcgcca ttgaactgaa ctaaggtctg
     8401 gggttgatat gcaatacatc aagatccatg cgctggataa cgtcgcggtc gctttagcgg
     8461 atttggctga aggcacagaa gtcagtgtcg ataaccagac tgttacgctg cgccaggatg
     8521 ttgctcgtgg acataaattt gcgttaacgg atatcgcaaa aggggccaat gtcatcaaat
     8581 atggcctgcc gattggttat gcattggcgg atattgcggc gggggaacac gtccacgccc
     8641 acaatacgcg cacgaatctg agcgatctgg atcagtatcg ctatcaacct gattttcagg
     8701 atctgcctgc gcaagcggca gatcgtgaag tgcagatcta tcgtcgcgct aacggcgatg
     8761 tcggggtgcg taacgagctg tggatcctgc caaccgtggg ctgtgtcaac ggcatcgcgc
     8821 ggcaaatcca gaaccgtttc ctgaaagaga ccaacaacgc cgaaggtacc gacggcgtgt
     8881 tcctcttcag ccacacctac ggctgctcac aactgggcga cgatcacatc aacacccgca
     8941 ccatgctgca aaacatggtg cgccacccga acgcgggcgc agtgctggtg attggtctgg
     9001 gctgtgaaaa caaccaggtt gccgcattcc gtgaaacgtt gggcgatatc gatcctgaac
     9061 gcgttcattt catgatctgc caacagcagg atgatgagat cgaagccggg atcgagcatt
     9121 tgcatcagct gtataacgtg atgcgcaacg ataaacgcga gccaggcaaa ctcagcgaac
     9181 tgaagtttgg tctggagtgc ggtggttctg acggtctttc tggtattact gctaacccga
     9241 tgctggggcg tttctctgac tacgtgattg ctaacggcgg tactaccgta ctgaccgaag
     9301 tgccggagat gtttggcgca gagcagttgc tgatggacca ttgccgcgac gaagcaacgt
     9361 ttgaaaaact ggtgaccatg gtcaacgact tcaaacagta ctttattgcc catgaccagc
     9421 cgatctatga aaacccatcg ccggggaaca aagcgggcgg tatcaccacg ctggaagata
     9481 aatcccttgg ctgtacccag aaagcgggtt ccagcgtcgt ggttgacgtg ctgcgttacg
     9541 gcgagcgtct gaaaacgccg gggctgaact tgttaagtgc gccgggtaac gatgccgtag
     9601 cgaccagcgc cctggcgggt gcgggctgcc atatggtgct gttcagtact ggtcgtggca
     9661 cgccatacgg cggctttgta ccgacggtga aaatcgctac caacagtgaa ctggcggcga
     9721 agaaaaaaca ctggatcgac tttgacgcgg gtcagctgat ccacggtaaa gcgatgccgc
     9781 agttgctgga agaatttatc gacaccatcg ttgagtttgc caacggtaaa caaacctgca
     9841 acgagcgtaa cgacttccgc gaactggcga tcttcaaaag cggcgtaacg ctataaagtc
     9901 gaaatgagtt gttaataaaa aacggcgttt cataatgtga agcgccgttt gcttttccat
     9961 acctgataag gatttatcat gaccgcgtat tggctggccc agggcgtggg tgtcatcgcc
    10021 tttctgattg gtatcacaac atttttcaat cgtgacgaac gtcgcttcaa aaagcagctt
    10081 tcggtctata gcgccgttat tggcgtacat ttttttcttc tgggcaccta tcccgctggt
    10141 gccagtgcca tccttaatgc cattcgtaca ttgattacct tacgcacgcg cagcttatgg
    10201 gtaatggcga tttttattgt gctgactggc ggaattggcc tcgcgaagtt ccatcatcct
    10261 gtcgaactat tgccggttat cggtacgatt gtcagtacct gggcgctgtt ccgctgtaaa
    10321 gggctcacca tgcgctgcgt gatgtggttt tcaacgtgtt gctgggtgat tcacaacttc
    10381 tgggcggggt cgataggcgg cacaatgatt gagggtagtt ttctgcttat gaatggcttg
    10441 aatatcattc gtttctggcg gatgcagaaa aggggaattg atccgtttaa agtagagaaa
    10501 acccctcccg ccgtagacga aaggggttaa acaattaatt acgcagggcg ctatttgcca
    10561 gacgatcgtc ttccgcctgg caagctgccg cagtgaacag cacgtcagtt gaagagttca
    10621 gcgcggtttc gcaagagtcc tgcaatacgc cgatgataaa gccgacggca accacctgca
    10681 tggcgatatc gttcgaaata ccgaacatat tacaggccag cgggatcagc agcagagaac
    10741 cccccgccac gccggatgcg ccacaggcac acagagaagc caccacgctc aacagcagcg
    10801 ccgtgggcag atcgaccggg atacccagcg tattaaccgc agccagcgtc aacacggtaa
    10861 tggtgattgc tgcgcccgcc atattgatgg tggcacccag cggaatagaa acggaatagg
    10921 tatcgcgatc cagattcagc ttttcacaca gtgccatatt caccggaatg ttcgccgcag
    10981 agctgcgggt gaagaaggca tacacgccgc tttcgcgcag gcacagcagc accagcggga
    11041 acgggttacg acgaattttc caccacacca gcaatgggtt aaccaccagc gccaccagta
    11101 acatacagcc aaccagcacg accagcagtt gcgcgtagcc ccacagtgtg gagaaaccgg
    11161 tggttgccag ggtagaagaa accagaccaa aaataccaat cggtgcgaag cgaatgacca
    11221 gtttcaccat aaaggtgacg gcattcgaca tatcgttgac caggtttttg gtggtctcgt
    11281 taccgtgacg cagtgcgaaa ccgaggccaa ttgcccacac caggatcccg atgtagttac
    11341 ctttcagcag cgcgtcgatg gggttggaaa ccatgctcat taccagcccg cgcatcactt
    11401 ctacaatgcc tgacggtggc gaaatatcac ccgcgctact ggataagtgc agggtagaag
    11461 ggaaggcgaa gctgaaaacg actgcggcca gagcagcaga gaaggtaccc agcagataga
    11521 ggaacaaaat agggcggata ttggttttct gcccgtgctg gtggttagca atagatgcca
    11581 tcaccagcat caacaccagg atgggggcaa cggctttcag tgcgccgacg aacaaagtac
    11641 ctaacagacc aacagcttcc gccgcgggtt ttgagatcca tgccagaaga atccccagaa
    11701 caaggccgac caggatttgt tttaccaggc tgccatgagc cagacgccgg aatagccccg
    11761 gtgaacgttg cgtagtcatt tttcgatcct ttcattgtgt tgtcgcacat ccctggtgcg
    11821 ttctgtcgac gcatctttca ggatgtaaca aagtgtttgc aaggtcgagt ataaggaaag
    11881 ttacagcttc gggaagcgaa aaatgctgga ttttaaagag aggtcatatt ttgcaactta
    11941 atggttacat ttatttgaca taaaaataaa gccaacgtgc ggcttccaga ctgatgacaa
    12001 catcaaaaaa ggactcggtc agtcccctcg ccccgtttgg ggagagggtt agggtgaggg
    12061 gaacaggcca gcatcggtgc gaaaaattac cctcaccccg accctctccc tggaagggag
    12121 agggagaaaa cggtgctcat gttaatgaca gggtacacgg cttaggcaga ttaaaaatta
    12181 tccaccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa acgtcatcac
    12241 cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga agtcgacaat
    12301 cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga gcatcgagaa
    12361 acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga ttgcaaacag
    12421 gtttgaggtc agcacaataa acggatcagt ggtcacggcg aagatagccg gaatgctatc
    12481 cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca gcggtgtggc
    12541 gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt cggtcatgcg
    12601 caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt catcttcatg
    12661 ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat acaggatcca
    12721 gtcgaactgt gaaatcagcc agctgcctgt gaagatcatg atggtacgca gaacaatcgc
    12781 cccgagcacg ccatacacca gcacgcggcg ttgtaatgcc gccggaacag agaaatagct
    12841 gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa tcagatagcc
    12901 cgtgagaaag gccagtgcct gtggatcggc aacggcgcga ccttcagttt gcaccagata
    12961 ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg accaggccgc
    13021 agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca ggtcgatagc
    13081 cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc cgacagtatt
    13141 catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag ccgctgcttt
    13201 ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg aatacgtctg
    13261 cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcacacatcg taatgacgat
    13321 cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac cattcagcca
    13381 gtcaatggtc tgttacatac cttttacact gttttacttt ggcaattata gttttacgct
    13441 atctgccgga aaaatcaccc cagtctggcg gcggatctcg gtcagcagct tggcggtgat
    13501 gcgactgacc gccagccccg gatgatccac cagatgctca tccaccaggg tagcgaacag
    13561 ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct gcatttggct
    13621 gccacgcggc acgaagcaca ctttctggca ttcagacagt ttttcaatca ccagcgatcc
    13681 tgcttcgccc tgaatctcgc tcgccaggac agaatcactg acttttgagt gctgcaaggt
    13741 gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcatctacac cgcttgccag
    13801 caaactggcg gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg ccgacgccag
    13861 gcagtaaaag ccgatatcca taattgaacc gttagagaat gacggattaa aggtgttggg
    13921 attttcaccg tccaggtaac gttgatagcg cgaggaatac tggcaatagt tgaaaaagac
    13981 tttacgcaat ttgccgactt tcggcagcgc ctggcgcagc aaatgaaagt tcggcaggca
    14041 ggcggtttta aatgcctcaa acagcaccac ctgattttcc cgcgcacagg caatggcggc
    14101 atccacttcc gccagattcg acgccagtgg tttctcgcaa atcacatgaa ttttatggct
    14161 aaggaaaagt tgtgtctggg aaaagtgcag ggcattcggg ctggcaatat acaccgcgtc
    14221 aatggcatcg ctttccgcca tcgcttccag cgaggtaaac agatgctcga cagaaaaatc
    14281 attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta acttgtattt
    14341 accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac caatcacagc
    14401 gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat gtagcacgcc
    14461 tttttcatct tcgacgtgcg cagatacgca ttattctttt gccggacagc catgctcact
    14521 aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga ggattttggc
    14581 gtcaatcacg gcgctgacac gagcgagttt ctcccgcagt tgagtataaa aaccgtgctt
    14641 catattgagg tcaggcgcgt taaaaccgat agcgtcgatg ccgtactgtt ttgccagcca
    14701 gatggcgcgt tgattatgaa attcttgcga gataatggtg atatggtttt caccaaaaac
    14761 ctttttggct cgcactaccg aatccagcgt tgagaatccg gcatagtcgc agaagatcac
    14821 tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgaggctt catcgtaatt
    14881 tttacgtccg ttatcgccac ttaccagtag ccatttcact tttccggcgt ggtacaacgc
    14941 tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctggccc ccagcaataa
    15001 gcccacgtta cgcgccggaa ctgcgttgac atcgctccag gtcagctgct tgctggcgtt
    15061 gaccataacg cgatcggcaa taaaaatggt cgctcctgct ataagcaaca acaaacagcc
    15121 aatcttaaga gtccggcgag aaaaacaaat acggagaaga aggcggggct gcatggtcgt
    15181 tatccttaac gaaagggggg aggtcgattt taggcgctaa agaaagaaaa gataacgcct
    15241 gacatcattt tcagattttg tcctgcccaa gcgacaactg cgttagccac agccgggtgt
    15301 cgaactcaag ctggtggtac tgcggctcca tatggcagca caactggtaa aacgctttgt
    15361 tatgctcttt ctccttcagg tgcgccagtt cgtgcaccac gatcatacgt aaaaacggtt
    15421 ccggcgcgtt gcgaaacaca gtagcgacgc ggatctctgc ttttgctttc agcttgccgc
    15481 cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt ttttagcaca tggatcttat
    15541 tgtcatacat cactttattg atcggcgggg cattgcgcag aaactgattt tttagatccg
    15601 gagtatattg ccagagggct ttatcggtgg cgtagtcgtg tgttcccgga tagcgttttg
    15661 ccagcacatc gcccagacgc tgttcgttaa tcagcgttcg cacctgggaa agtagcagct
    15721 cgggataacc ctgaagataa gtaagattgc tcataaccgc ccacgaaaaa gagaaaaagg
    15781 gtatactcac gcaccctttt caggggcaac gtcgaaattt tatcattcag gagggccgat
    15841 gagccactta gacaacggtt tccgttcact gacactacaa cgttttccgg cgacggatga
    15901 cgttaacccg ctacaggcgt gggaagcggc ggatgaatat ttgctgcaac agttggacga
    15961 cacagaaatc cgcggcccgg tgttgatcct gaatgatgcc tttggtgcgt taagctgcgc
    16021 gctggcggaa cataagccgt acagcactgg cgactcatac atcagtgaac tggcgacgcg
    16081 cgagaattta cgcctcaacg ggattgatga gtcgagcgtg aagtttctcg acagcaccgc
    16141 cgactacccg caacagccgg gtgtggtgct gatcaaagtg ccgaaaacac tggcattgct
    16201 ggaacaacaa ctgcgtgcgc tgcgcaaagt ggtcacgtcg gatacacgta ttattgccgg
    16261 tgccaaagcc cgtgacattc acacttccac gctggaactg ttcgaaaaag tgctcggccc
    16321 gaccaccacc acgctggcat ggaaaaaagc gcgcctgatt aattgcacgt tcaatgaacc
    16381 gccgctggcc gatgcgccgc agaccgttag ctggaagctg gaaggtactg actggactat
    16441 ccataaccat gcgaatgtct tctcccgcac cgggcttgat atcggcgcgc gcttctttat
    16501 gcaacatctg ccagagaatc tcgaagggga gattgtcgat ctcggttgcg gtaatggcgt
    16561 tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa gtggtgtttg tcgatgaatc
    16621 gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc aacatgccag aggcgttgga
    16681 tcgcagcgag tttatgatca acaacgcgct ctccggcgtg gagcctttcc gctttaatgc
    16741 tgtgctctgc aacccgccgt ttcaccaaca acatgcgctg accgataacg tcgcctggga
    16801 gatgttccac catgcccgcc gctgcctgaa aatcaacggc gagctgtata ttgttgccaa
    16861 ccgtcacctg gattacttcc ataaactgaa gaagattttc ggcaactgca ccaccatcgc
    16921 cacgaataat aaatttgtgg tgctgaaagc agtgaagctg gggcgtcgtc ggtaagattg
    16981 ggagttacct gccggatgcg gcgttaacgc cttatccggc ctacgttcag cacgttactc
    17041 ccaggccgga taagacgcgg caagcgtcgc atccggcaat gtctgcccag ttctggaatc
    17101 gcgcttccat aaaacaaaaa atccctgtca ccatcctcta cattctcttg tttagcgttg
    17161 ttcaacgttt atgctacata cgtgaagtta tgacgcataa agaatacgat ttctttaccg
    17221 ctgttcatcg tactaagggg aaaaaatgat tgcgattgcc gacatcttgc aagcaggaga
    17281 aaagctaact gctgtggcac cttttctggc gggtattcag aacgaggaac aatacaccca
    17341 ggcgctggaa ctggtagatc atctgctgct caacgatcct gaaaacccct tgctggatct
    17401 ggtgtgtgca aaaataaccg cgtgggaaga atcagcgccc gaatttgcgg aatttaatgc
    17461 catggctcaa gccatgcctg gcggtatagc cgtgattcgt acccttatgg atcaatatgg
    17521 tttaaccctt tccgatctgc cggaaattgg cagtaaatct atggtgtcac gcgttttgag
    17581 cgggaagagg aaattaacgc tggaacacgc taaaaaattg gcaacgcgat tcggcatttc
    17641 tcccgccttg tttattgatt aacgcgacgt gccggatgcg ttgcatccgg cgcttcaaat
    17701 aacgatttaa atctccagcg ccagccgtgt tccctgggca attgcccgtc gtgcgtccag
    17761 ctctatagcc acatcgcagc cgccaattaa atgcacagtt ttcccgctat caatcagcgg
    17821 ttgcgccagc gcgcggtttg gctcttgccc tgcgcagatc accacattgt ccactgctaa
    17881 tacctgcgtt tcgccgttga tcaccacatg cagcccgtca tcgtcaatct tctgataact
    17941 tacgcctggg atcattttca caccccgcga gagcagggtg gtgcgatgga tccagccggt
    18001 ggttttgcct aacccctgtc ccggtttgct ggctttgcgc tggagcatca caatttgccg
    18061 tgggctacgc gggatctgca ttccctgcgg gcttaagcca ccagcctgtt gtaggctgct
    18121 gtcgatcccc cactcattac agaacctggc gatattctgg ctggtggatt cgcccggctg
    18181 acttaaatac atcgccgtat caaaaccaat cccgccacaa ccgatgatgg caaccttgtt
    18241 gcctaccggc gctttgtcgc gcagtacatc gagataactc aatactttcg gatgatcgat
    18301 cccgtcgata ggcggaacgc gcggcacgat cccactggcg aggatcgttt catcgaacgc
    18361 ctgtaactga tccgccgtca cggtgtgatt gagtttcagc gtcacgcccg tcacttcgat
    18421 catccggtgg taatagcgca gcgtttcgta aaactcctct ttgccgggga tctgtttggc
    18481 gatattaaac tgcccgccga tctcgctgtg ggcatcaaac aatgttacct gatgcccacg
    18541 cgccgccgcg ttaatggcaa acgccagccc agcaggtccc gcaccgacca ccgccagatt
    18601 ttttttctgc acggcaggaa ggattggcat tttggtttcg tggcaggcgc gaggattcac
    18661 caggcacgag gtgactttgc caacgaagat ctgatcgagg caggcctgat tgcagccaat
    18721 gcaggtgttg atctcatcgg ctcgccccga ttgcgctttt gacagcagtt ccgcatcagc
    18781 aagaaacggt cgtgccatcg ataccatatc ggcatcgccg cgcgagagaa tgtcgtcggc
    18841 aacctgcgga tcgttaatcc ggttggtggt caccagcggc agcgaaatgt ggcctttcag
    18901 tttgcgcgtg acccaagtaa atgcgccgcg cggcacgggc gtggcaatgg tcggaatacg
    18961 tgcttcatgc cagccaatgc cggtgttgat aatggtcgcg cccgccgctt caatggcctg
    19021 cgccagctct accgtttcgg caaaagtccc gccgtcttcg accaggtcga gcatcgacag
    19081 tcggtagata ataatgaagt cgttgccgac gcgttcgcgc accgcacgca ctacttctac
    19141 ggcaaatcgc atccggttgc ggtaatcgcc gccccactgg tcactacgct gattggtgcg
    19201 cagcgtcaga aattcgttga tcaaataccc ttcggacccc attacctcta caccgtcgta
    19261 tcctgcctcc cgtgccagtt gcgcgcagtg ggcgaaatcg tcgatcagtt gcaggatctc
    19321 ttcatgggtg agttcgtgag gaacgaaacg gttgatgggg gcctgcaatg cggagggagc
    19381 gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa atttgcaggg ctattttgcc
    19441 gccttcctga tgtaccgctt cggtaatggt gcgatggtgt gggatctggc tggcgtcgtt
    19501 gagcattgcg ccaccttcca tgccaacgcc tgttaaatct ggtgcaatgc cgccgctgac
    19561 aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac gctgccagcc gctcggcacc
    19621 gtccgggtat tcctccagcc cggtgtgcat tgagcccatc aacacgcggt tttttaacgt
    19681 ggtaaaacct aaatccagcg gggcgaacag cgacgggtag ctcataaaat tgtccagtat
    19741 gtaaaataat tgttatgtgg tcggatgagt tctaatttag ccgtcgccgg agaaaaggga
    19801 aaaggggagt gcggggtttg tgatgggatt caaaaagaat acgcccggtg gtaacgccgg
    19861 atgcgacgct tgccgcgttt tatccggcct gggggattgc acatgtaggg cggataaggc
    19921 gttcacgccg catccgccag tggcacggtg cagatgccgg atgcgacgct tgtcgcgttt
    19981 tatccggcca ggaggattgc acatgtaggg cggataaggc gtttacgccg catccgccag
    20041 cagttgcaaa ttattgctta cggaaaaaat cgttatacaa catatacaaa tgcgcggcac
    20101 tccaggagaa atttggtgcg ccttgctgtg cgccagtcag cgggttgtaa ttctcctgaa
    20161 ttgggccatc ggcggttaat cctttggcgt gacggaagaa cgtatccgcc agtttcaggg
    20221 catcatcacg ataaccgtaa cgctccatcc ctttcagacc aaaccagaac tgatccaccc
    20281 atacgcgccc gcgccagtag atatcagcgc caaaagccgg attggttaac gccgccgttc
    20341 ccagcgggac aaaggtgttg aactctttag gatcgagcat caccttcacc accgcgtcgg
    20401 cgttggcctg cgttgccgca ccgttaaaca gcggcgacca gccttccggc cctttaccgc
    20461 gctcgacaat cggtttgccc gcgcagccgt tcgccagcgg tttatcttca atacgcacgt
    20521 catagtagaa ctgggtagcc gggtcgaaca tacaggtgtt tatgtagtct gcgagctgct
    20581 gtgccaactg gcgatagcgt ttggcctctt ccggcttacc gaggatggtt gccatctccg
    20641 ccagataatg gttatcgctg tacatatagc tggcctgatc caccgactcc tgcaatagcg
    20701 agtagcccag caacgttccg tcctgactgc ggttttcggc gaatttcacc gtccagtcgc
    20761 tacgtttgcc gccattagcg acatatttat ccaactgttc tttgtcgata aaaccaaaga
    20821 cggcggcatc atcacgcccc gattcccacg acgcagcgac ctgtgccgga atttccagac
    20881 tgtcgtactg gcctttctcc accacgcggg cgtagttgtt cagcccagac tgcgtctctt
    20941 ctttgttgcc tttcttcacc gtaaacagca tctcgccgct ctcagtgttg tgggctttgt
    21001 cgcgggtcgc gccatattcc ggcacgccgt tgccgttgtg atcgcggtta cgtaaccacc
    21061 agtcgtgata ggccaccagt ttcgggtaca tctctgccaa ccaggcttta tcctgggtga
    21121 cgttgtacac ttccatcacc gaccaggcgg caaggctggg tttggtatta cgttcgttcc
    21181 agttgccgcc atcgccacca cgctcggggc taaggttcca cgctatcagg tcggggacaa
    21241 agcccacatc ctgcggacgc acgctatcgc caggctggat ctgccaggag aagaccgcgc
    21301 ggatattctc tttggcgatg tccggattga aatgcgccat cgcaaacgcc tgcttccagg
    21361 tatcccacgg ccaggtctga ttgccggaga accagcgccc ggtcaccgac ggtgtaacgg
    21421 tgttaaattt caccgcaccg cccggtgagc gccagttacc gttgagcgtt tcgatcgctt
    21481 tcaccgcgac gcgcgtctgt tccggcgtcg catccggatt ggttaaccct ttcttcagat
    21541 actcttccca gcgttgctgc gaggcggtga gataaaacgc cggacgcgcc agaatatcgc
    21601 ggatctgcat ttgctctttg ctaacttcct gagaggtcag cagatgggaa taggtggtat
    21661 agagcgtcgt cgaaccgttg atatgtgcct tactggtaaa gcgattgccg ttgatttcag
    21721 tctgcaccgg cagggattta tgcacctgat attccgattc gccggaggtc agcagatccc
    21781 aggtggcgcg cactttgcca aaggtgactt tcaggccatc acgggtggcg ctgattttgc
    21841 gctggtagtc agggtattcg ccagcaatgg ttttatcgga aagcggtttc ccttctttcg
    21901 cttccagttt ttccagcagt tcgccgtccc acaccagatc cagcggttta ttgctggtga
    21961 ttttggtttc cagtagtgac gtgcgcggcg tggcgaagcg cagagtcatt tcgacttgca
    22021 catcttttgc tgtcagtttt tgcaccagcg caccgggaat actgtatgcc tccagcgtaa
    22081 agtcgacttt cttgccgtcc tgccagacgg tcaggcggtc gaaattgctg gccataaagt
    22141 tgatgtactc ttccgtcagc agcgcaacgc ccggaaagcc gcccatggtg ttagggccgt
    22201 ctggcaacag atgaccatgc caggctccga gatcgaaaaa cggattaaaa cgctggtgat
    22261 cgtcgtaatc gtaatccttc atgtactgcg gcgcgccagt acggttaatc acgtttttat
    22321 aattgtcggc gttagcggca tgggcggaaa aacttatcag cagagcgcag gttactggcg
    22381 ttaaaatagt ttttattttc atgaaattac agtccttatt accagtagaa atattgcatc
    22441 aggaagattt ggtcgctggg atcgtgagag gcatttttca tgtagaagcg gctatcaaaa
    22501 gccgtggcga aacgtccgcc gccgtattcg taccagatac caaactgcat gaaagaggtg
    22561 gtttcatcgt cagcgtggtc agggcgatgt ttggcgtagg tgtaagcggt ggagagataa
    22621 acgcccttta acgcttcata catggcgctc accatcatcc cactttcact tcccggtacg
    22681 tccagcccgt cggcgcgtcc ggtatggtgc caggcgcgag cggcaaagtt aggagttagc
    22741 aggcccgtga gatagagctg tcctgtgccg tcggtgattt ccagaccgtt catccaggtg
    22801 atgccttcgg caaggtcata ctggatgtaa ccgttcatca ttgccggata ggtgtatttg
    22861 tcgtcatagc gatcgtactt gccaaaatgt agccaggctt tgctttcgtc atgatgtccg
    22921 gcaggtgtgg cggtgacgct ataacgaaag ttgccgctta agttctgcac cttcagcgcg
    22981 gtcataatgt cgcgggtatt gggaatgacg tagcccagat cgggggtgaa gtcgccccac
    23041 cattgcaggt catccagcga ggaatcctga cgcagactga gcatgatttc ggtgccatca
    23101 ttggtacgat aaccgccata gaaacggtta attccgccct cgaaaccacc ccagctatgg
    23161 tctggcaccc aggcattgcc gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc
    23221 aggccgaacc aggagccgta cttcatctcc aggccacctt caatataggt accgtcgctg
    23281 tatttatgtt tattctcacc ctctaaatcc atatatccac caacccctaa ttcgccgtaa
    23341 aaacgaaatg aaggagaaga tgtcgaggta attgcaggtg tttttgtgtc gtcatgttct
    23401 gccggacgtg cttcttctcc tgccgcagaa aaggcgtagc aaaacggcag agcaagtaat
    23461 gctctgcatg gtgcagtgat taatttcatt gtgttaatcc atgagctaaa gaaaaaatac
    23521 agccttttat cgttgtaatt ataaaaatat aaaggcagaa actattttcc ctctcctcat
    23581 ccaggaagag ggaaaatgcc tgctcatttt ttattattct gcttaatcaa cattgctggc
    23641 tggcgtcgct tcaatatggc gctcttcagc cgttaatccc tttatatatt tactgtattt
    23701 ccaccacgca aagccaagga agataacaat accgccgacg ttataaaaaa tgatggtcag
    23761 aatattcgcg ccagtcggga atgtcgaagc gacaaaccct acggcaaata tcgcaatcag
    23821 cattgaaaca acgataatac cggtgcggcg ggagcccata cggaaatcgc gtggcaggtg
    23881 atctaatttg gcgcgtaaat tcaggtaagc cagcatgatg aataacggcg gaagcatgga
    23941 cgctgcggcg gtcatattaa taatagtatt catcagatcc tgcacggtat tggaaccgag
    24001 catcgggata atcatcagcg ggatgacgat caggaactgg atccacgctg cgcgcgccgg
    24061 aacgccgttt tcgttcagtt cgacggtttt cttaccaaag atgccttccg ggatttcgga
    24121 gaagaaaatt ttcaccggcg ttgcggtcca catcagcagg gaaccgaaca tcgcggtaaa
    24181 ggacaccaga ccgacaaagc gattcatcaa cgcttccggt agaccaaaat acgccgccat
    24241 gccgtggaat acctgcaccg atccgccggt aaatttcaac tctttgctgc tgacgaagac
    24301 gttaatcagc accgaggaga cggaatacag tacgccgata aaaatcccgg cgaggatgat
    24361 cactttaacg aacgatttcg aaccgccttt gacgtcgtta acgtacacag cgacggactc
    24421 cgcgccacct gcggccataa agatccaggt ggtaacgccg aggaacgccc agttgaagtt
    24481 cgggatcatc gcgtcaacgg tgatggggtc agcaggctgt acgccgccaa ccagcgccgt
    24541 acctgccagt aaaatgtagg agagcgttaa cagcagcatc agcgttgaag tgacggaggt
    24601 aattggcccc aacattttcg ccccgttggt ggaaacccag gtggagaagg cgaacagcac
    24661 catactgaga atggtggtgg caaccggcgt cataatatat tcgtatccga ggaaggcata
    24721 cgaagcataa gcaataacgc gcggcaacag tgaggtgaaa aagaacaggt ttacgaacca
    24781 gtaggtatag gcagtaataa atgcccaacg tccgcccagc gaacttttta cccacgcgta
    24841 gacaccggct tctgagtttt tatttaacga aacaaattct gcgatgatca gacagaaggg
    24901 aataaaataa aaaatcgtcg cgaggaaaaa catcggtgcc gaggccagtc caagctcaat
    24961 attattattg ataacgttgt taaagctgta aacggcggca aaagtcagcg agagcaagcc
    25021 gaatttgccg attgtattac gtttggtatc agacataaca catctccgaa aatgtgtagg
    25081 gtagcggtgg cggcaaactc cttttgccgc cgtagttgtt atttgttatg gaaataacca
    25141 tcttcgatgg tgactttgag aaccactttt ttgaccgcgt tattgcagat aaaccgatac
    25201 gcttcatgga tatcgcaaat aacgatttgc ccttcgtgga cctcaacggt ttctccgcag
    25261 ccttttaaat attcacggtc agtttcatca cgataatatt ccactacctg taatgtctct
    25321 ttcggtgcat attcaatttt ttgctgccct tgcaggtagt aatgcacttc aaaatagcgt
    25381 cgatgcccgg taaatagcgc atcggtcgcg gagtctgtct ccacgcggta agtcaatgag
    25441 tcaccgatgg agtgggcgac gccaggctga atgttgtcga tattttcaat cgcttcaacg
    25501 cagcgttgcc acttcttgcc agaggcgtaa atctggcgga actgttctaa gttatcgatg
    25561 atcctcattg cttattctcg ctgtgcaaat tcgtggaaaa gaaccctgcg cctaactcat
    25621 acgacgccag gctttgcgcg gtagcttctc cgccagaaac cggcagcaac gtaaagccgt
    25681 agctgaagtc acggaaccag acgcgccagg agtccagcac ctcgctgccc caggagttgg
    25741 agccgaggcc aagtagctgg tggtcgaggt tcagggtgat gtcatcactg cgctgcagct
    25801 cgttacagtg ctgggcagcg tggatgtttt cctgggtata gtgccaggcg ctgaagttaa
    25861 ttgggcgctg cggaaccacc agcagaccgt tgccgtggcg gttagtcagt gccgtccagc
    25921 ggacatgctg acggttgccg ttgttctgcg ggaagggata gttctcgaac atggcatcga
    25981 cggtgctgcg ccagatatcg atgatgttag cctgctggct gtcggcgtag ttttcgcccg
    26041 gtccacgacc gtaatacgcc acctgatcgt attcgccgtt aatccccatg gtgaaaccga
    26101 tgcacggaat gatgtgcgga tagtcgccgt aacgctcgcc ggaaagcgcc acgttaacct
    26161 ggccatcggc agcgatgcgc cagatgtagg tgcagcgcat cccgaagtca aacaccggcg
    26221 gggcaataac tgtgcggctg atgatcaaca cttcaccatc gctctgttct acggcaaagt
    26281 cgcgcagatg ttcctgcatg atctgcaaat gattcggttg ccacagccct tcgttctcct
    26341 gcttgtggtt gtcgatcatt ggcttgaaga agttgatctt tggctcgcgg gtcagcagcg
    26401 actcgccatt cacctgccag gatgtcggtt tgccactcat ttttgagaag gtgatcgcga
    26461 agttgtagcc gcgaacggtg cagctcaaac gatcgtcttc cagcgtcagc ggacgcgcat
    26521 tatttggtgc gaaaggcact ggttgcgcgg tgttttcctt cagcgggaac tgataagtgg
    26581 cgatagaatg cccggcttcg ctgtagcggg tgcgggaatc tttggtcacc gtaatgttga
    26641 ggaacgcttc gcgggcgtcc agctgcggca gcgtgatctg caagggggct tcgctgttcg
    26701 gcgcaacgtc gcgcagttta atctgctgcg tcgcgagcgt ttcaccttcg acgcgcacct
    26761 ctgcgtgcag ggtgtagtca tcaagcgtgg taaaccacag tttattttcg actttcagct
    26821 cgccgcgagt cagatccagc gcgtggattt ttaccggcgc gataacctgt ttgtactctt
    26881 tcaggcccgg tcccggcgtc tgatcggaat agatcaaacc atcaagacag aagttatagt
    26941 tgttgggata gtcgccgtag tcgccgccga atttatacca gacgttgccg ttgtcatcct
    27001 gcgcctggat cccgtggtcg caccactccc aaacataatg tccctgaata caatcgtgct
    27061 tatagaagac gttctggtac tccgtcagcc cgcctggtcc gttccccatc gcatgagcat
    27121 attcacagat gatgcgcggc ttcggatgcg ggtattcacc aaactcattc atcagcggca
    27181 cgcgggtgta catggtggaa ataatatcga ccacttcagc atcgcgatct tcttcgtaat
    27241 gcaccagtcg cgtgtcatcc agcgccttcg ctgcgtggta catcgcgcgg atgttacagc
    27301 catagccgga ttcattgccc agcgaccaga tgatgatcga cggatggttt ttctgcgcgt
    27361 gaatatggcg aacaatgcgc tcgacgtaga ctttttccca ctgcggatcg tcggtaatac
    27421 ggctgatatc gccgacatta gcaaagccgt gcgattcgac gtcggtttcc gccatcacaa
    27481 acaagccgta gatatcacac agttcgtaaa aacgcggatc gttcgggtag tgagcggtac
    27541 gcacggagtt gatattgtgc tgcttcatca actggagatc tttctcgacg cgatccattc
    27601 caacggcgcg gcctttgcga tgatcgttgt cgtgacggtt gacgccgtgc agcatcacat
    27661 aacggttatt gatccagaac agaccgtcgc gcactttgat atcacggaag ccaacgcgtt
    27721 gtggcaccac ttccagaacg ttgccgttgg cgtctttcag cgtcatgacc agatggtaaa
    27781 gataagggga ttctgctgac cattgctgcg gctgttcgac agtaaaagca aagctggcgc
    27841 tggtcagttt ttcaattgcc aaatgatcaa tggcgctgct gtgcaccacg cgttcgccat
    27901 caaacaaggt atattccagc gtcgtgacga caggggaggc ggcgagattt tccagcacca
    27961 cttcgcagga aagcgtggca tcgcaatagg cttcgtcaaa gtcggtacgc acagtgaaat
    28021 cgttaatatg cgttaggtgt tttccgacca gataaacatc gcggaagatc cccgctgacc
    28081 accacatatc ctggtcttcc acgtaggtag agtccgccca ctgcatcacg cgcacacaca
    28141 acaggttgtc gccggtttta accatcgcgc tgatgtcaaa ctctgcggtc aggcgactgc
    28201 ccttgctgaa acccacatac tgaccgttaa catagacttc aaaataggtt tcgacgccgt
    28261 caaatttaat cagcgtctgt ttaccctgcc agccgtcgct gagggtgaaa atacgttgat
    28321 aggcaccggt tgggttatcg ctggggacaa acggcacatc gatggggaac ggaaaacctt
    28381 cgtcggtata ttgcagtttg ccgtgacctt ccatttgcca catggcgggg acggtaatat
    28441 gcccccagtc agccattaac tcagaggtga aggcttctgg tacttgcagc ggatggtcaa
    28501 aaaagtggaa attccactga ccgcttaagg gcagaaacag gctgctggtt tcgcgggcaa
    28561 aggtacgcgc ttgcgcaaca gaatcatatg aaaaaaagta cgcacgcggc gcaagtcggt
    28621 tttcgtgggt gagctgaatg ttttcccagc gattcatagg gcctcccggt agagagaaat
    28681 cggaaccatt catggtgatg gtagtttgtg taattgctgg ggagtttagt aaaattttta
    28741 ctaaaaagta gcaggtaaat gcatttactt taactggatc acgaaaataa acggcagcac
    28801 tgtccggcaa aaacgattgc cggacagtgt gtaaggggat ttaacgggtc gtgccgcgca
    28861 gttttaattt gctgggaacg aagactaaca gcggcagcgc gcgaccatcg cgggcttttt
    28921 catacaccag gttaacgccc tgacttccca tcatctcgga atggatgcgc acggtggaga
    28981 gcggcggaaa ggtaaatcgc gcggtgggga tatcgttaac gctgataagc gaaatatcct
    29041 gtgggatgtt caggcctcgt tcatgaattg cccgcagtac gccgatagca atggaatcgg
    29101 aagcaacaaa cagtgccttc ggatagtctt cccgcgccag catttgtttt gccagttcat
    29161 aacccgacga actggaaaaa ccgccgcgcc agatatcctc ttcgcgtacc acttgtttca
    29221 gtcggccata ttccgcaaag gcgacctcac gaatatccgc cttgccaggc tcatcttcac
    29281 cgccaataaa accaatacga ttaacgccct ggttgatata gaagtcgatg atttctttac
    29341 tgatgcgtgc cagatcgata tccaccgcat cgtaaccgct gccgggttcg tgaaagtcga
    29401 taaaacagat attgtcggtc aacgcgctgg cagcggcgcg cagggcgggc gtgggtttgc
    29461 cgacaattaa aataccggtg acgtttttaa tgtctggtaa gccgctgtgt tcataacagt
    29521 tggtgagctc gatgcccagc ttttcgcact gggtttcaat gccgtggcgg atcgccagat
    29581 agtaaggatc gttgatctcc agctcctgct ggtagctgta gatagccaga atatggtgtt
    29641 ggttgactgc acctgtctgg agtttacggg cactactggt cttgtactcc agcttttcgg
    29701 cgatctcgag aatgcgatgt ttcgtctctt ctttcacatt caatgtcgga tcgtcattta
    29761 agaccctgga tactgtcgcc agggatacgc cagcttcgat tgcgatgtct tttagtgttg
    29821 ccatgtttac cttttttact ttccctgatg tgcgccccca ttgtagagga agtgcttatc
    29881 gggtcacctg ttttcagtaa ataaagcgcg tttctttgac gctgtaacgg taacacagcg
    29941 gctcacgaat gtgatcacgc ctgcattctc catttttagc ctacaggatt gagaaaaact
    30001 tttgccatag tttagtaaaa ttttacccaa agggaggaaa caatggaaac cgtggcttac
    30061 gctgattttg cacgtctgga aatgcgcgtc ggaaagattg tggaagtgaa acgccatgaa
    30121 aacgccgaca agctgtacat cgtacaggtt gatgtggggg aaaaaacact gcaaaccgtg
    30181 accagcctgg tgccgtacta cagcgaagaa gagctgatgg ggaaaacggt ggtggtctta
    30241 tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg aatgcatgct gttgtgtgcg
    30301 gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg agcggatgat gcctgcgggc
    30361 gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc gccatccgat ttgatattac
    30421 gcttcttcga cacttactcg catggccgcc agcgccttac gcgccgcttt gatcaccagt
    30481 tcacactgtt caatggtcag tgtcagtggc ggttcaatgc ggatcgtttt ggcgttattg
    30541 agcgttccgg ccaccagtac gcgctggcgg aacatctcgc tggcaaagtt atagccgatt
    30601 tcgttatcaa caaactcaat tgccatcaac atccctttac cacgcgcttc ctgtaccaga
    30661 tcgggatatt cccgcgccag ttgacggaaa ccgtccagca acatatcgcc tttttgctca
    30721 gcctgagccg gtaagttctg ctccagcaac acattgatgg tcgctagcgc cgccgcacag
    30781 gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg ggttgtcgaa cagaactgaa
    30841 aacacctctt cagtggcgat ggtcgcgcca atcggcatca cgccgccgcc gagcgctttg
    30901 gcaaggcaga ggatatccgg ctgtacgttc tcatgctcgc aggcgaacat cttgcccgtg
    30961 cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg cgccgaactc atcgcatagc
    31021 ttacgtacgg cggtgagata gcccggcggc ggcagaatta cgccaccttc accctgaatc
    31081 ggttcgagga tcaccgcagc cacatcatca ccggtttttt tgcactcgtt aagagccgtg
    31141 cgcatggctt cgatattgcc aaacggcaca tgacggaagc ccggcagcag tggcataaac
    31201 ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc caagtgattt accgtggaac
    31261 gcgccgctgg tggcaataaa agtaaacttg ccgcgcggtg actggtaagc tttcgccagc
    31321 ttcagcgctg cttcaacgga ctcggtgccg ctattacaga agaagctgta tttcagttta
    31381 ccgggcgtta gcgcagcaag ggttttcgcc aacatcgccc gtaacggatc gagcagctcc
    31441 tggctgtgca gcggttgttt cgcaagttga ttctgtacgg cggaaaccac aactggatta
    31501 cggtgcccca cgttgaaaat tccaaaacct cccaggcagt cgataaactc ctgtccctgg
    31561 gtgtcgacaa gcgtatttaa acctcccgct tgccactcta cggctccgta atccccgccg
    31621 gcggtaacag atttgcgata ctctaaaaac cccggattga catgctcttt gaagtattca
    31681 atcacctctc ggttaagtgc tttcatctcc tcatgatcca gcgttcgctt ctcaatgaga
    31741 ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg aaggtaacct gttcaaaata
    31801 tgctccggag gctcgcgtat cacatgatac cgatttaagt attgcaggga ttgcgccact
    31861 ccggctgcga tcgcgaaaat cgggataaac acaaattcgg ttatcatttg tgcaatattt
    31921 aatgctatct gttaacattt gttgttattt tgcacggttt ttgtcgggta tggatttgat
    31981 gattgctgcg caaatgcacg ttttcagggc aataattgcc tgatttttgg gcaagataat
    32041 taacgttttc gtatagtaac agagtgaaag tattttaaat caaagaatta gatttgtgct
    32101 ttaatcgcaa attgcgatct aaatcaaatt aatcggttaa agataaccgc agcggggccg
    32161 acataaactc tgacaagaag ttaacaacca tataacctgc acaggacgcg aacatgtctt
    32221 ctcatccgta tgtcacccag caaaataccc cgctggcgga cgataccact ctgatgtcca
    32281 ctaccgatct gcaaagctat atcactcatg ctaatgacac ttttgtgcag gtgagcggct
    32341 ttaccttgca agagttacaa gggcagccgc acaacatggt gcgtcacccg gatatgccaa
    32401 aagcggcgtt tgcggatatg tggttcaccc tgaaaaaagg ggagccctgg agcggcatcg
    32461 tgaaaaatcg ccgcaaaaat ggcgaccatt attgggtgcg ggccaatgcg gtaccgatgg
    32521 tgtgcgaggg aaaaatcagt ggctatatgt cgattcgtac ccgggcgacg gatgaagaga
    32581 ttgcggcggt ggagccgctg tacaaagcgc tgaacgccgg acgtaccagt aagcgtattc
    32641 ataaaggcct ggtggtgcgt aaaggctggc tgggtaaact gccttcatta ccgcttcgct
    32701 ggcgggcgcg tggagtgatg accctgatgt ttatcttgct ggcggccatg ctttggtttg
    32761 ttgctgcccc ggtggtgacg tatttcctct gtgtgttagt ggtattgttg gcaagcgctt
    32821 gttttgaatg gcagattgtg cgcccgatag aaaatgtcgc ccgtcaggcg ctgaaggtgg
    32881 cgaccggaga gcgtaatagt gttgagcacc tgaatcgcag cgatgagctg gggctgacat
    32941 tacgcgcggt agggcagctt ggcctgatgt gccgttggct aattaacgat gtctcaagcc
    33001 aggtgtccag tgtcagaaat ggcagtgaga cgctggcgaa aggcaccgat gaactgaacg
    33061 aacataccca gcagacagtt gataacgttc agcaaacggt ggcgaccatg aaccaaatgg
    33121 cggcgtcggt gaaacagaac tctgccacgg cgtcggctgc cgataaactt tctatcaccg
    33181 ccagtaatgc ggcagtgcag ggtggggagg cgatgaccac ggtgatcaag acaatggacg
    33241 atatcgccga cagtacccag cgcattggca ccattacttc gctgattaac gatattgcgt
    33301 ttcagaccaa tattctggcc ctgaatgcgg cggtggaagc ggcgcgtgcc ggcgaacagg
    33361 gcaaaggttt tgcagtggtg gcaggggaag tgcgtcattt agccagccgc agcgctaatg
    33421 ctgccaacga tattcgcaag ctgattgatg ccagtgctga taaggtgcaa tccggttcgc
    33481 agcaggtaca cgccgccgga cggacgatgg aagatattgt ggcacaggtg aaaaacgtca
    33541 cccagttgat cgcccagatt agccattcaa cgctggaaca ggccgatggg ctttccagcc
    33601 tgacccgtgc agtggatgag cttaacctga tcacccagaa aaatgccgag ctggtggaag
    33661 agagtgcgca ggtgtcggcg atggtgaaac accgcgccag ccgactggaa gacgcggtga
    33721 cggtgctgca ttaatcggta tgccggatcc ggcggttgga gcacaatgcc tgatgcgatg
    33781 ctggcgcatc ttatcaggcc tgcggggtgt gtagcgggtg taggccggat aaggcgcgtc
    33841 aagcgtcgca tccggcaatt gcaccgcgcc actggcggat gcggcgatta atccagttgc
    33901 gtaatatcaa aagcggcgcg gtcgatcacc gcaatgatct tttttatttg tgcatcactg
    33961 atatcgctct ggttgacgtg taaatccagc accgctttaa aattatccag cgcccgcttc
    34021 atttgcgggt tctggcgcag cgccgcgcca acgcaacgcg ctttgatgcg ttcttcaatc
    34081 atctccacct gttcgcggtt ttcttccagc cactgcgcgc cttgttcggt cagcgcaatc
    34141 tgcttcttac caccttcctc ttcgcggatg gtaatcagcg actgctcctg cagaaaatcc
    34201 agcgttgggt agatgacgcc cgggcttggg gtgtaattcc cctgggttag gttctcaatc
    34261 gctttaatca attcgtaacc gtggctgtca tcgcgcgaga gaatatccag aatcaccaga
    34321 cgcaattcac cgtgaccaaa gaaacgttgc cgacgaccgc cgccgcgacc gtggcgacca
    34381 ccgcagcatt gaccgtgttc atgctggtga ccgtgtccgc agtgctcgtg ttctgacttc
    34441 tcacctttgc agcagccctc atggcgtggc tggccttcat gtttacaaca cccttcgtga
    34501 tgatggctca tatcagcctt cttttgcttg attagatata tctaatttat atctgatgtg
    34561 agcattactg caagtacaaa aatgattttt aaacgtggaa gcacctcaca tccaggccgg
    34621 ataagacgtt cacgccgcat ccggcagcat tgctatggca ctgaatgcct tcacatactt
    34681 cagatatatc agttctacaa atcgcttgca tttatcatga ttaacaatca ttatcattta
    34741 cgaaaatcat ttagatatat ctgattacta tcacgaaggc gataacaatg aataactccc
    34801 cccgctaccc gcagcgcgtt cgcaatgatc tgcgcttccg tgaactgact gtgttacgcg
    34861 ttgagcgcat cagcgccggt tttcagcgca ttgtcctcgg cggcgaggcg ctggacggtt
    34921 ttacatcgcg cggctttgac gatcacagca aactcttctt tcctcaacct gacgctcact
    34981 ttgtgccgcc aacggtaacg gaagagggca tcgtctggcc ggaaggacca cgcccactgt
    35041 cgcgtgacta tacgccgctg tatgacgaac tacgccatga actggcgatc gatttcttta
    35101 ttcacgacgg tggggtctcc agcggctggg cgatgcaggc gcaaccgggc gataaactta
    35161 cggtggcagg tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg
    35221 tctgcgatga atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg
    35281 ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc
    35341 tcgcgcacct tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg
    35401 cgcgtctggc gcagatgcaa atccctgccg atgattactt catctggata accggcgaag
    35461 gtaaagtcgt taagaattta agccgccgct ttgaagcgga acagtatgac ccacagcggg
    35521 tacgtgcagc ggcttactgg cacgcaaagt aactgatata taaaaagaaa aaggctgacg
    35581 atttctcgtc agcctttgct atatctggtg gcccctgctg gacttgaacc agcgaccaag
    35641 cgattatgag tcgcctgctc taaccactga gctaaggggc catggtagcg gattataaag
    35701 taactccgtg tcgcaatcca gccattaccg tgcgcctgct gtttttataa acaatgtatt
    35761 ttcaatccgt tatacttttc ctggtgatgt tagaaaggag taaatatggt tgaggatatt
    35821 ttggctccag ggttacgggt cgtgttttgc ggtatcaacc ctgggctttc atccgccggg
    35881 actggttttc cctttgctca tccagcaaat cgcttctgga aggtgatata tcaggccggg
    35941 tttaccgacc gtcagttgaa gccgcaggag gcacagcatc tgctggatta tcgttgtggc
    36001 gtcaccaaac tggtagaccg tccaacggtg caagccaatg aagtttcaaa gcaggagctg
    36061 cacgcaggtg ggcgtaagct gattgaaaaa attgaagatt atcagccgca ggcgttggcg
    36121 attctgggca aacaagcata tgaacaggga ttcagccagc gcggtgcaca gtgggggaaa
    36181 caaacgctca ccattggttc gacgcagatt tgggtgctgc caaatcccag cggtttaagt
    36241 cgcgtttcac tagagaaact ggttgaagca tatcgcgagc tggaccaggc gctggtagtg
    36301 cgtgggcgat aaaaaacgcc accgaaaagg tggcgtttgt gcaaagggac tagtgccggg
    36361 tgcggcgtaa cgcctgatcc ggcctaccga ttaatcgtcc aggaagctac gcaacacttc
    36421 agaacggctc gggtgacgca gtttgcgcag cgccttcgct tcgatctgac ggatacgttc
    36481 gcgggtaacg tcgaactgtt tacccacttc ttccagcgtg tggtcggtgt tcatatcgat
    36541 accgaaacgc atacgcagaa ctttcgcttc acgcgcggtc aggccagcca gcacgtcgtg
    36601 cgttgccgca cgcaggcttt cggtggtcgc agaatccagc ggcagctcga gggtggtatc
    36661 ctcgatgaaa tcccccagat gcgaatcttc atcatcaccg atcggcgttt ccatggagat
    36721 tggctctttg gcgatcttca gcactttgcg gatcttgtct tccggcatca gcatacgttc
    36781 agccagttct tccggcgtcg gttcacggcc catctcttgc agcatctggc gagaaatacg
    36841 gttgagtttg ttgatagtct caatcatatg caccggaata cggatggtgc gcgcctgatc
    36901 cgcgatagaa cgggtgatcg cctgacggat ccaccaggtt gcgtaggtgg agaacttgta
    36961 accacggcgg tattcgaatt tatcaaccgc tttcatcaga ccgatgttgc cttcctgaat
    37021 caggtcaagg aactgcaagc cacggttggt gtatttcttg gcgatagaaa taaccagacg
    37081 taagttcgct tcaaccatct ctttcttcgc acggcgggct ttcgcttcac cgatggacat
    37141 acgacggttg atatctttaa cctgctcgat ggtcaggccg gtttcttctt caatctgctg
    37201 cagtttctgc agggcgcgat gcacttcttc agagacatcg tgcagttttt ccgaccacgg
    37261 cttgttcatc gcaattgccg cgttgaacca ggtatcgctg gtttcgttgc cggtaaacag
    37321 ggtaatgaag tttttcttcg gcattttgca ctgctcaacg cagagcttca tgatcagacg
    37381 ttcttgcgta cgaacgcggt ccatcatgac gcgcatgctg ttgaccaggt agtcaaactg
    37441 cttcggcacc aggcggaact gtttgaatac ttcagacagt ttcaggatct cttcctgagc
    37501 ggcagcgtga ctgcgacctt tcgctttgat ggtgtcacgc gttacaacgt actgagcgcg
    37561 tagttccgca aatttttcgc gagccagttc cgggtcgatg ctgttgtcat catcggcgct
    37621 gtcgtcatcg ccatcttctt cgtcttcatc ttcgtcatcg tccagatctt cctgggaaag
    37681 ctcagaaccg acgtgagtgg cggtaggtgc cagatcttct tctgcgttcg ggtcaacaaa
    37741 gccggtgatc agatcggaca gacgcgcttc ttctgcttca acacgatcgt actgttccag
    37801 cagataggtg atcgcttccg gatattcagc aacggagcat tgaacctggt tgatcccgtc
    37861 ttcaatacgc ttagcgatgt caatttcgcc ttcgcgggtc aacagttcaa cggtgcccat
    37921 ttcacgcatg tacatgcgta ccgggtcagt cgtgcgcccg atttcagatt ccacgctgga
    37981 aagcacctgc gcggcggctt cggcagcatc ttcgtccgcg gtgttttcag ccagcatcag
    38041 atcatcggca tccggtgctt cttccatcac ctgaatgccc atgtcgttga tcatttggat
    38101 gatgtcttcg atctgatctg aatcgacgat atcttccggc agatggtcat tgacctcggc
    38161 ataggtcaga tagccttgct ccttaccacg ggtgacaaga agtttcagct gtgactgcgg
    38221 gttttgctcc ataagacggt atccacactt atttcatgag gttggtgttg ggcgattgac
    38281 ccgatgtcct tcagccgtta acgctgaaat ccaacggttg tttaccgcca acgataatta
    38341 cgagggcgta cttatatatt tgccgctgcc tctcagtgcg gctgtcgggg gcttcccgat
    38401 cgctcttcgg cacttaagcc gttaaatcac tttttcgcca gctccaggtt taatgtccag
    38461 agctccaggc gttcttcgtt gcttaaacca tgcgtgcgct cacgagcgat taactcttcc
    38521 tggcgcagtt caagcagcga atcaaacata tggttgagtg agtcggtgaa ggtttgctca
    38581 gcaatattct tatctgctat atcgtcccac atcgacagtt tttcaagggt ggcagcatta
    38641 tttgtaccac gatagtgctc taaaagttgc ccggtggtca gacctggctg ggagagacaa
    38701 gtgttgacca gttctctgaa taagccaagt ccagggagct tattttcatc cagattctca
    38761 agcggcggga ccaacgtcgc taattctgga ttttgcacca gcaaccctat aagtatacgc
    38821 atggtcgtgc gttttagctg cggaacaggg cgagaaacgc cgctctctgc cgcttttggc
    38881 attaatcgtt caagctggct gtcatcaagt atgcctaatt tgttgcctaa ttcctgacga
    38941 agatatattc gcagcgtttc gcccggcact tgcgatatca atggcagtgc cagcgtactc
    39001 aaacgtgcgc gcccgtcagg ggtactcaga tcaacttgcg gcatcaggct gttaaacaga
    39061 aatgcggaga gtggcatcgc ctgctccatc cgcgcttcaa acgcttcttt accttctttt
    39121 cgtaccagcg tgtcagggtc ttcgccatcc ggcaaaaaca taaagcgtag ctgacggccg
    39181 tctgtcatgt aaggcagcgc cgtttccagc gctcgccatg cggcatcgcg gcctgcacgg
    39241 tcgccgtcat aacagcaaat gacattgttg gtcgcgcgga acaacagttg tatgtgatcg
    39301 gcggtggttg acgtacctaa cgacgcaacg gcgtaattaa tgccgtattg cgccagcgcc
    39361 accacgtcca tatagccttc gaccacaagc agacgattgg gttcagcgtt atcctgctgc
    39421 gcttcataaa gaccgtaaag ctggcggcct ttatggaaaa tgtctgtttc cggcgagttc
    39481 aggtatttgg gggtatcgtt gcccagcacg cgcccgccaa aaccaatcac ccgaccgcgt
    39541 ttatcgcgaa tggggaacat cacccgctcg cggaaacgat cgtaactgcg tccctgatcg
    39601 ttagtgacca acatccccgc atcaatcaat gactggcgat tttctggatt gccgccaaac
    39661 cgcttcagga cgttgtccca gccggggggc gcaaaaccaa tcgcaaagcg agcgataacc
    39721 tcgtggctta atccgcgttt ttccagatac tggcgcgcag acgtggcaac aggttgttgt
    39781 aaagattgtt ggtaaaacgt attcagaccg tccatcaact gataaagcgt ttgcctctga
    39841 tggcgctcga tctggctggg gccgctgcct gcttcaaatg gcacttcaag attgtgcatt
    39901 gctgccagct cttcgaccgt ttcaacgaac tcgagcttgt cgtagttcat caggaagtcg
    39961 atcgcgttgc cgtgcgcgcc acatccaaag cagtggtaaa actgtttctc accgttaacg
    40021 gtgaatgacg gggttttctc gttgtggaat ggacaacacg cgtggaaatt cttgccctgc
    40081 tttttcagct tcacacgggc atcgatcaga tcgacgatgt cagtgcgtgc cagcagatca
    40141 ttaatgaata cgcgtgggat tcgtccagcc ataggccccg atttttagca attcataaac
    40201 gaaaataagc cgcgcattcc tttcggaagc acggccttac aactacaact cggtctgatc
    40261 ggagagcaac gctctcgggg aattagtaca gacgagtgcg gcgtgcgttt tcgcgagcca
    40321 gtttcttcgc gtgacgtttc actgcagaag ctttagcgcg cttacgttcg gtagtcggtt
    40381 tttcatagaa ctcacgacga cgaacttccg ccagaacacc tgctttttcg caggaacgct
    40441 tgaagcgacg cagagctacg tcgaacggct cgttttcacg tactttaatt accggcatgt
    40501 gcctctcacc tttgattaat tcggtttgcc gctggcatca acgccagctt atttcaaaat
    40561 ggtgcggaat tttactgcaa ttgctgctgc tttgtaaagc accgcggcct tttttgaaag
    40621 ggacttttat aagggtgagg agtatacacg aggctttctc caggggcgaa caaagtttta
    40681 catcaacccg cattggtcct acactgcgcg gtaataaagc gaggtaaaac aagtcatgcg
    40741 tgtactgggt attgaaactt cctgcgatga aaccggcatc gccatttacg acgatgaaaa
    40801 aggtttgtta gccaaccaat tgtatagtca ggtgaaattg cacgctgact acggcggcgt
    40861 cgtgcctgaa ctggcctccc gcgatcacgt gcgcaaaacc gtaccgttga tccaggcggc
    40921 gctaaaggag tctggtttaa cggcaaaaga cattgatgct gtggcctata ccgcaggccc
    40981 tggattagtc ggcgcactgc tggttggcgc aaccgtgggg cgttctctgg cgtttgcctg
    41041 gaacgttccg gcaatcccgg tacaccatat ggaagggcat ctgttagcgc cgatgctgga
    41101 agataacccg ccggaatttc cgtttgtcgc gctgctggtg tccggcggtc atacgcagtt
    41161 aatcagcgtg actggcattg gtcagtacga gctgctcggc gagtctatcg atgatgccgc
    41221 cggtgaagcg tttgataaaa ccgcgaagct gctggggctg gattatcctg gcggaccgtt
    41281 actgtcgaaa atggcggctc agggtactgc cgggcgcttt gttttcccgc gtccgatgac
    41341 cgaccgtccg gggctggatt tcagcttttc tggtctgaaa acctttgcgg cgaacacgat
    41401 tcgtgacaac ggcaccgacg accagacgcg tgctgatatc gcccgcgcct ttgaagatgc
    41461 ggtggtcgat acgttgatga ttaagtgtaa gcgagcgttg gatcagacgg gctttaagcg
    41521 actggtcatg gcgggcggcg tgagtgctaa ccgcacgtta cgggcgaagc tggcggaaat
    41581 gatgaaaaaa cgccgcggcg aagtgttcta cgcgcgtccg gaattttgta ctgataacgg
    41641 cgcgatgatc gcctatgccg gaatggtgcg gtttaaagca ggcgcgacgg cggatctcgg
    41701 cgttagcgtg cgtccgcgct ggccactggc agagttaccg gctgcgtaaa actatccggt
    41761 gccggagagc aatttccggc accgtcctca cttaaagcaa caccacgggc atccacagca
    41821 agccggtaat caccagcaac acgaggaata tcagtccaaa aatcgccccc agtcgccagt
    41881 aatccaccgt tggcagataa ccactaccgt agtaaatcgg actgggtccg gtggcgtatg
    41941 gtgtaagaat gctccccaga ccaattgccg cgccaaccat caggcagaac accggcagcg
    42001 ggatttccgg catcgccagc gcagcggcaa tcatcatcgg tgcgagggcg gaggtatacg
    42061 ccgtggcgct ggcgaaaaag tagcgcagca gataaaacac cacaatcaac gccaccatca
    42121 ccatcgttgg cgaataaccg cttaagctgc ctgctaacag tttgccaaac cagctaataa
    42181 aaccagtgtt gttgagtccg gtagcgaggg tgataagcga ggccagccag aagaaaacgt
    42241 tccacgccgc tttattactg acaatgtcgt cccaactgat aatgcgcaga agcagcatca
    42301 gtgccaccac gctgtaaccg accatcgcgg catcgatata atcaccgccg aaaatccaca
    42361 gcaccagcgc gcctaccatc agccccagca tccgtttttc acgcgaacag agcgggccca
    42421 ttgcctgcag ttccgtctct gcccagcgcg gcacctgatc gccagacttc agtaccggcg
    42481 ggtacagcac ataagccagc cagggaacca gcagcaccag caaaatactg agcggcaaca
    42541 tcccgaggaa ccagtcgccc caactcagcg tggcgtgaga tgcgcttttc atcagtccaa
    42601 ttaacagcaa gtttggtgcc atcgccgtca agaaaatggc gctggtcacg cagtcggcaa
    42661 caatccccat ccacatgatg tacgagccaa tgctgcgcga actgctgtcg tttggttgtg
    42721 attgatagag cggtggcagg ttacggatga tgggatagat aatccccgca ccacgcgccg
    42781 agttggacgg tgttacaggt gcgaggatca gctcggagaa catcaccgca tagccgagaa
    42841 acagcgtgcg atgtcccatc tttttcacca gaaacagcgc aatgcggcgt ccaagcccgg
    42901 ttttttcata gcctgtgcca aacataaagg cggcgaaaat cagccagata accgaattag
    42961 aaaaaccgga aaccgcccac gagagggatt ttgcagtgaa tttaaagcct ggctgggcga
    43021 gctgttcggg gctgaacagc agccagggag agagtatggc gatgatggag atacccacca
    43081 tcgccaccac ggcacccggc acgggttcga ggatcagtcc aacgatcacg ccagtaaaaa
    43141 cggcaaagta gagccaggta tggttctcca gccctgcggg aaccggaagt agagcaataa
    43201 tggcgatgac cgccagcggc gcaagatatc gccaccattc agtggaaggt ttcatcgtgt
    43261 tatgcctgcg aaaaaggaga ggggtgagcc tctccgggag ggttatttga tgtaatggac
    43321 gtgctcgcag atctcttcca cgatgggatc gcggcgttcg gcgaacagct ttttgttttc
    43381 ggctatcagg ttgttgccgt gggtatcaat agagacaatc agcgggccga actctttgac
    43441 ccggcagacc cacagtgact ccggcatccc gagttctgtc cagtgcactt cttcaatctc
    43501 ttctacctgg gttgccgcca gcaccgcgca gcctgccggg aaaatcacat gtagcgcctt
    43561 gaatttctgg cagccttctt cggtcagcgg ccccatacca cctttgccaa ccaccagttt
    43621 cacgccggtc tgctcgataa attcacgttc aaaactttcc atacgcatac tggttgtcgg
    43681 gccaacggaa accatctccc atttatcgcc gtttttgcgc acgatggggc cagcgtggaa
    43741 aatcgctttg ccgttgagat cgtaagggat cggacgtttc agttcgatca agcggcggtg
    43801 acaaacgtca cggcaggtca ccagcgtacc ggtcaggtag atcacatcgc cgacgcgaat
    43861 atcttgcaga tcttcagctt tgatcggggt tgtcaggatc tttttcataa cgcgctccgg
    43921 gtgtgagaca gattttcaaa ggtgagatcg gcatgaacca gcagtgtgcc gcgacgatgc
    43981 gcccagcagc ctgtagaaac agcaacgccg atggttgacg gatggcgggc ggcagattcg
    44041 atatgcacgc ccatcactga actgttgcca gtcagccctt gtgggccaat tcccagacgg
    44101 ttgagtcctt cttccaggcg tagctccagc tctgccgctt ttggattggg atggcggctg
    44161 ccaatcgggc gcaaaatggc ttttcgcgag agcacggcgg cggtttccac cgaggtggcg
    44221 atgcccacgc ccaccagtac cggtggacag gcgtttacgg cgagggtgga gatattttcg
    44281 aagacgaatt tcaccacgcc ttcgtagcct tctgacggca ttaacacttt cgagcggcca
    44341 ggtagcgtgc agccgccgcc tgccatgtaa acttcgattt ccgcatcgtc attgtcgggg
    44401 atgatgtccc aggtgaccca cggtacgccg ctaccggtat ttttgccggt gtttacttcg
    44461 tcaaaaattt ctaccgcatt gtgacgtagt ggcgctttga cggttgcctc ttccacggct
    44521 tgtttgagta tgctttgcag ctcgccaagc agtgggaagc gggaaccgac tttaacgaag
    44581 aacataatct ccccggtgtc ctgacaggca ggacgattca ggtcaatcgc tttttgcatg
    44641 ttgtcgaaca tcgtatggta gataattttc cccatcgacg acgtttcggc atcctttagc
    44701 tgttttagtt tatccaccac gtcatcaggc attcgggtag aaatcatggc ggtaaagtta
    44761 gcgacaatct ctgtcaactt attcactgcc tgttgcttat tactttcgct catcatggca
    44821 actccagttt ttgtatttgt ctggaaaaat tcaccgccac aaactaccac taaaaatgtg
    44881 gttttatatt ttttgccacc gtgaatatgt aattaacagt tcgtgaatca acgcgaaaat
    44941 aagaatgaat attgcgccag agcagggaat ggcaatgttt tgcgaaatac actgcgctta
    45001 tcacaactgc ggataattaa cgatgctgaa tagctggcct ttagccaaag acttgcaggt
    45061 actggtagaa atcgtccaca gcgggagttt tagtgctgca gcggcgacgc ttgggcagac
    45121 gcctgcattt gtcaccaagc ggatccaaat ccttgaaaat acgctggcaa caacgctgct
    45181 gaaccgctcc gctcgcggcg tggcgctgac tgaaagtggc caacgttgtt acgaacatgc
    45241 gctggaaatc ctcacccaat atcagcgact ggtcgatgac gtcacgcaaa tcaaaacgcg
    45301 cccggagggg atgattcgta ttggttgcag ttttggtttt gggcgcagcc atattgcgcc
    45361 agccattacc gaacttatgc gcaattatcc tgagttacag gtgcattttg aattgttcga
    45421 tcggcaaatt gatttagtgc agcataatat tgatctggat attcgtatta atgacgaaat
    45481 tcctgattat tatattgcgc atctgttaac gaaaaataaa agaatattat gcgcagcacc
    45541 tgagtatctg caaaaatatc cgcagccaca atcattacag gaattaagtc gtcatgactg
    45601 tctggtgacc aaagagcgcg atatgaccca cggaatatgg gagttgggga acggtcagga
    45661 gaaaaaatcg gtgaaggtta gcgggcatct ttcctccaat agcggcgaga tagtgttgca
    45721 atgggcgctg gagggcaaag ggattatgct gcgttccgag tgggatgtgt tgccgtttct
    45781 ggagagtggc aaactggtgc aagtattgcc ggagtatgca caaagcgcca atatctgggc
    45841 tgtttaccgg gagccgctct atcgcagcat gaaattacgt gtctgcgtgg aatttctggc
    45901 ggcatggtgc cagcaacggc tgggcaagcc cgataaaggc tatcaggtca tgtagatcca
    45961 ccagaaatca ctcgggatcc ttttcgcgct ttcttttgaa tttcgtccag atttttgtct
    46021 cctgacgacg ccacagacgc tggatgttgt catgatgacg cagcaggatc aggcaagaga
    46081 gcatcgaaac cgggaaggtg aattgtggct taaaccacca tacataaaac ggagcaatca
    46141 gtgcactgac aatcgctccc agcgacgagt atccgctcaa tagcacggtc agtaaccagg
    46201 ttcccgccat tactccggtg agatcccagc caatgggtgc gatggcacca aaagcggtag
    46261 caacgccttt tcctccttta aatccgaaga aaacgggcca gatgtgtcca agacaggcgg
    46321 cgatggcaat taagcctagc cagaaggggc tgacacctaa ttcatacgcg ccccagacgg
    46381 gcaacattcc tttcagaacg tcgaaaatca gtactgctac ggctgctccc ttgccaccga
    46441 tacgtaacac attggttgcg cctggattgc cggagccgct ggttcgcgga tcgggcagcc
    46501 cacacaagcg gcaaaccaga atggcactgg aaatggagcc gcagaggtac gcgatgagga
    46561 tcattccagg cgcgattgca ctcataagct gttccgtttt gaaaattcgt gttcaacgat
    46621 gaatctgtgg ataatacgca catttcgccg gaagtggtat ccggttagcc aaaaagcagg
    46681 caggacgtga tggatattgt atttatagag caactttcgg taatcaccac tattggtgtt
    46741 tacgactggg aacagaccat cgaacagaag ttagtgttcg atatcgaaat ggcatgggat
    46801 aaccgtaaag cggcgaaaag tgatgatgtg gcggattgcc tcagttacgc tgacattgca
    46861 gaaacggtgg tcagccacgt cgagggggcg cgttttgcgc tggtggaacg cgtggctgaa
    46921 gaggtggcgg agctgctgtt agcacgcttt aactcgccgt gggtgcgtat caaactcagc
    46981 aagccaggcg cagtggcgcg ggcggcgaat gttggcgtaa tcattgagcg tggcaataat
    47041 ctgaaagaaa ataattaatt ttacagctgt taaaccaaac ggttataacc tggtcatacg
    47101 cagtagttcg gacaagcggt acattttaat aatttagggg tttattgatg agcgatatgc
    47161 actcgctgct gatagcggca atattgggtg tggtcgaagg attgacagaa tttctgccgg
    47221 tatccagcac gggccatatg attattgtcg gtcacttgtt ggggttcgag ggcgacacgg
    47281 cgaaaacctt tgaagttgtg atccagttag gatcaattct ggcggtagta gtgatgttct
    47341 ggcggcgtct gtttggcctg attggcatcc actttggccg cccgttgcag cacgaaggag
    47401 aaagcaaagg tcgtttaacg ctgatccaca ttttgctggg gatgattccg gcggtggtac
    47461 tggggctgtt gttccacgac acgattaagt cattgtttaa cccgataaat gtgatgtatg
    47521 cactggtcgt tggcggtttg ttgctgattg ccgccgaatg cctgaagccg aaagagccgc
    47581 gtgcgccggg tcttgatgat atgacctatc gtcaggcgtt tatgattggc tgtttccagt
    47641 gtctggcgct gtggccgggt ttctcccgtt ccggggccac catttcaggt gggatgctga
    47701 tgggcgtgag ccgttacgct gcttccgagt tctcgttcct gctggcggta ccgatgatga
    47761 tgggcgcaac ggcgctcgat ctctacaaaa gctggggctt cctgacaacc ggcgatatcc
    47821 cgatgtttgc cgttgggttt atcaccgctt ttgtggtggc gctgatagcg attaaaacct
    47881 tcctgcaatt gattaagcgc atttcgttta tcccgttcgc catttatcgc tttattgtgg
    47941 cggctgcggt gtacgtcgtg ttcttttgat tgagaaaagg ttcgggagca ggcctggttt
    48001 tctccctcgc tctgtaggga gagggccggg gtggggggga ttgttcgcac cggtgctggt
    48061 ctgttcccct aaccctctcc ccgaaggggc gaggggatgg ttcgtgttaa cgtttagttt
    48121 tatactcagt caggctttgg gcaacgttgt tccttccaac tggctaccgc cgcaatccgc
    48181 cgtcgggtca gctcctcgcg aatctccaca cctttaaatc ccgcttcaac gaccgctttt
    48241 gtcggcactg actgcgccac ttcccaggct tcgcgcaacc aacggccttg cgggtagtcc
    48301 gctgattcaa aaccggttct gccgcgtacg tcagcctcgc tggtcagcgc cagttgctcg
    48361 acacgctgcg gtttacgcca ggcatcgatg gaatcaaata atttgacgat ggtttttggg
    48421 ttcagcattg ggaaggtgtg gatgagatcg tgaaactcag ccaccagtct ggctaaatcg
    48481 cgaatttcat ttggcacgcg cagacgctgg catagttgtt ccactaactt aacacccgcc
    48541 gggccatgcc catgatgacg cggccaaagt tctggcggcg tcagtccttt accgagatcg
    48601 tggcataaag ttgcgaaacg gacatcaacc tgcggactca gcatcgccgc cattgagagc
    48661 gtcattaagg tatgaatacc cgtgtcgatt tccggatgcc acctggcagg ggccggaacg
    48721 ccaaacaggg cgtcaatttc cgggaataaa acgcgaagtg caccgcaatc gcgcagtacc
    48781 tggaagaaca cctggggatt gcgggtggtg agggcgcttt ccgtctcttt ccatacccgt
    48841 tcaggcgtca ggtgttccag ttcacccgca tgggtcatct cgcgcatcag cgtcagagtt
    48901 tcatcggcaa tacgaaaacc gaggtgggca taacgcgcag caaaacgcgc cacgcgcaga
    48961 acgcgtaacg gatcttcgcc aaaagcgggg gaaacatggc gcaacagacg attttgcaga
    49021 tcgcccagac cgttgtacgg gtcgataatc tcgccgttat cgtcctgagc cagcgcatta
    49081 atggtcagat cgcgacgctt aagatcatct tccagcgtga catccggtgc ggcatagcaa
    49141 gtaaaaccgg tgtaaccgga accggatttc cgttcggtac gtgccagcgc atactcttca
    49201 tgcgttagcg gatgcagaaa cacaggaaaa tcgcggccta cctgctggta gcccgcgtcg
    49261 agcatctcct gtggcgtact gccgaccacc acccaatctc tgtctttgac cggtagccct
    49321 aacaatgcat cccgaacagc accaccgacc agataaatct tcacgccatc atcccttcgt
    49381 caaatacaca attcctaata ataagacagt gcaacgaaga aggcgattta gttcatccag
    49441 cgatctttgc gtttgcgcgt tgggatcagg tgtggcagta ccagaccgag cagcaagcca
    49501 agccccagca cgccaccgcc atacataaac cattgcatga tgatggtgcg ctgtttgtca
    49561 tccaactgta cgctggcggc atcgaccttt ttctgcgcga caatcagctc gtttttcaat
    49621 ttctgatttt cttcttttaa tccgttgatc acgctgtcgc tctgcgccac tttttgctgc
    49681 atttctgcgg tgcgctgatt ccaggtgtta tcgatattgg tgagtttatc ggtcagggtt
    49741 ttgacctgat tttccagatc tggcacacgg gagcgcaggc ttggctcagt gctaagttgt
    49801 ttcaacggga tccaggcggt acggccagag ctgtctttca cctgggcata attggtgttg
    49861 gcgtcagttt gtaataaggt cacttcctcg ccggcgttaa ccgtgcccac gaggcgataa
    49921 tgatctcccg gaccgctacg gacccaggta ttcagttcat cagaaacata gcgcgtttct
    49981 tcagcgtgtg agacggcagt ggcgctaagt gcgagtaaag ttaatccgat caggcgtaat
    50041 tttggcatca ggctgtcgtt attgtcatgg aaagtggaac gatagtagtg gcatcagtgt
    50101 cgctacgcaa agcattcgac atcaatcgga atcctctgtg tcaggtcttg ccactgcttc
    50161 aaacttttac gcccgtcaaa ttgcccgttg catggcaatt tggcgcaaaa tactatctac
    50221 tgacaaaaaa gatcgcatta agttcgccgt tcatcgtcac ttatgtgaca taaccgataa
    50281 gtaaaggcca tggctcagga aatcgaatta aagtttattg ttaatcacag tgccgttgag
    50341 gcgttgcgtg accatctcaa tacgctgggc ggcgagcacc atgaccccgt gcagttgctg
    50401 aatatttact acgaaacgcc ggataactgg ctgcgtgggc acgatatggg cttacgtatt
    50461 cgtggcgaaa acggtcgcta tgagatgacc atgaaagttg caggaagagt gacaggcggc
    50521 ttacaccagc gcccggaata taacgtggcg ttgagcgaac cgacgctcga cctggcgcag
    50581 ttaccgacgg aagtctggcc gaacggcgaa ttgcccgccg atctcgcttc ccgcgtacaa
    50641 ccgctgttca gcaccgattt ttatcgcgaa aaatggctgg tggcggtcga tgacagccga
    50701 attgaaatcg ctctcgatca gggggaggtg aaagcggggg aatttgctga acctatctgt
    50761 gagctggaac tggaactgct tagcggcgac acgcgcgcgg tgctgaaact ggcgaaccaa
    50821 ctggtatcgc aaaccggatt acgccagggc agcctgagca aagcggcgcg tggctatcat
    50881 ctggcgcagg gcaatccggc gcgtgaaatc aaaccgacca ccattttgca tgttgcggca
    50941 aaagccgatg tggaacaggg gctggaagcg gcgttcgagc tggcgttagc gcaatggcag
    51001 tatcatgaag aactgtgggt acgcggcaac gatgcggcga aagagcaggt gctggcagcc
    51061 attggccttg ttcgccatgc actgatgctg ttcggtggca ttgtgccgcg taaagcgagc
    51121 actcacttac gtgatttgct gactcaatgc gaagcgacca tcgcttctgc ggtttctgcc
    51181 gtgacggcgg tctactctac cgaaacggca atggcaaagc tggcgttgac cgaatggttg
    51241 gtaagcaaag cctggcagcc gtttttagat gccaaagcgc agggtaaaat cagcgattcc
    51301 ttcaaacgct ttgccgatat ccatctttcc cgccatgccg ctgaactgaa aagcgttttc
    51361 tgccagccgt taggcgatcg ctaccatgat cagttgccac gcctgacgcg tgatattgac
    51421 tcaatactgc tgctggcagg ttactatgat cctgtcgtcg cgcaagcctg gctggagaac
    51481 tggcaggggc tgcgtcacgc tattgcgacc gggcaacgca ttgaaattga acatttccgt
    51541 aatgaggcaa acaatcagga accgttctgg ttgcacagcg gaaaacgtta atcaggcaag
    51601 gataaaatcg cttatgaagc cgctctcttc accgttacag cagtactggc agaccgttgt
    51661 tgagcggctg ccagagcctt tagccgagga atcacttagc gcacaggcga agtcagtact
    51721 tacttttagt gattttgtgc aggacagcgt gattgtgcat ccagagtggc tgacggaact
    51781 ggaaagccaa ccgccgcagg ccgacgaatg gcagcattac gcggcatggt tgcaggaggc
    51841 gctcagtaat gtgagtgacg aagccgggtt aatgcgcgag ctgcggctat tccggcggcg
    51901 cattatggtg cgcatcgcct gggcgcaaac gctggctttg gttactgaag agagcatatt
    51961 gcagcagctc agccatctgg cggagacgct gattgtcgcc gcgcgtgact ggctgtatga
    52021 cgcttgctgc cgcgagtggg gaacgccgtg caatgcgcag ggcgaagcgc aaccgctgct
    52081 gattttaggc atgggtaagc tgggcggtgg ggaactgaat ttctcctcag atatcgatct
    52141 gatttttgcc tggccggaac atggttgtac gcagggcgga cgccgggaac tggataacgc
    52201 gcagtttttt acccgcatgg ggcagcggct gattaaagtg ttggatcaac caacgcagga
    52261 tggcttcgtc tatcgcgtgg atatgcggct gcgtccgttt ggcgaaagtg gcccgctggt
    52321 gctgagcttt gccgcgttgg aggattatta ccaggagcag gggcgcgact gggagcgtta
    52381 cgcgatggtc aaggcgcgga ttatgggcga tagcgaaggc gtctatgcta acgagttgcg
    52441 tgcgatgctg cgcccgtttg ttttccgtcg ttacatcgat ttcagcgtga ttcagtcgct
    52501 gcgcaacatg aaagggatga ttgcccgtga agtgcgtcga cgtggtttga ccgacaatat
    52561 caaactcggc gcaggcggca ttcgcgaaat tgaatttatc gttcaggtgt tccagctcat
    52621 tcgcggtggg cgcgaaccgt cgctgcagtc gcgctcttta ctgccaacgc tcagcgtcat
    52681 tgccgcgctg catctgcttt ctgaaaacga tgctgaacaa ttgcgagtgg cgtatctgtt
    52741 cctgcggcgt ctggaaaacc tgctgcaaag cattaacgac gaacaaaccc agacgcttcc
    52801 ttctgatgag cttaaccgtg cgcggctggc gtgggcgatg gactttgctg actggccgca
    52861 actgaccggg gcgctgaccg cacatatgac caatgtgcgc cgggtgttta atgaattgat
    52921 tggcgacgac gaaagtgaaa ctcaggaaga gtcgctgtcg gaacagtggc gtgagctgtg
    52981 gcaggatgcg ttgcaggaag atgacactac gccagtgctg gcgcatctta gcgaggatga
    53041 tcgcaaacag gtgctaacgc tgattgccga tttccgcaaa gagctggata agcgcaccat
    53101 cgggccgcga ggacgtcagg tgctcgacca tctgatgccg catctgctaa gtgatgtctg
    53161 tgcgcgtgaa gacgctgccg ttacgctgtc gcgcattacc gccttgctgg tggggattgt
    53221 tacccgcacc acctatttag aattgctcag tgaattcccc gcggcgctta aacatttgat
    53281 ttctctgtgt gccgcgtcgc cgatgattgc cagccaactg gcgcgttatc cattattgct
    53341 ggatgaattg ctcgatccaa acacccttta ccagccgacg gcgaccgatg cctaccgcga
    53401 tgagttgcgc cagtatttgc tgcgcgtgcc ggaagatgac gaagagcaac agcttgaggc
    53461 gctgcgtcag ttcaaacagg cacagctgtt acgcatcgcc gcagcggata tcgctggtac
    53521 gctaccggtg atgaaagtga gcgatcactt aacctggctg gcagaggcta tgatagatgc
    53581 cgtcgttcag caggcgtggg ttcaaatggt ggcccgctac ggtaagccaa atcacctcaa
    53641 cgatcgcgaa ggccgtggtt ttgcggtggt cggctacggc aagctgggcg gctgggagtt
    53701 aggctacagt tccgatcttg acctgatctt cctccatgat tgcccaatgg atgcgatgac
    53761 tgacggcgag cgggaaatcg acgggcgaca gttttatctg cgtctggcgc aacgcattat
    53821 gcatctgttc agtacgcgta cctcttccgg cattttgtat gaagtggatg cacggctacg
    53881 cccgtccggg gccgcgggaa tgctggtgac atccgcagaa gcatttgccg attatcagaa
    53941 aaacgaggcc tggacgtggg aacatcaggc gctggtgcgt gcgcgggtag tgtacggcga
    54001 tccgcagctc accgcgcact ttgacgcggt gcgtcgcgag attatgacgc tgccgcgtga
    54061 aggtaaaact ctgcaaacgg aagtgcggga aatgcgcgag aaaatgcgcg ctcatctcgg
    54121 caataaacat cgcgatcgct ttgatatcaa agctgatgaa ggcggaatta ccgatatcga
    54181 atttattacc caatatctgg tgttgcgcta cgctcatgaa aaaccgaagt taacgcgctg
    54241 gtcagacaac gtgcgcattc tggaactact ggcgcaaaac gacattatgg aagagcagga
    54301 agcgatggcg ctgacccgtg cgtatactac gctgcgcgat gaacttcatc atctggcatt
    54361 acaggaattg ccgggccatg tgccggagga ttgcttcacc gcagagcgtg aactggtgcg
    54421 ggcaagctgg cagaagtggc tggtggaaga atgaagtatg gtattatcgc gcgcaaattt
    54481 tgaatctctc aggagacagg aatgaaagta acgctgccag agtttgaacg tgcaggagtg
    54541 atggtggttg gtgatgtgat gctggatcgt tactggtacg gtcccaccag ccgtatctcg
    54601 ccggaagcgc cggtgcccgt ggttaaagtc aataccatcg aagaacgtcc gggcggcgcg
    54661 gctaacgtgg caatgaacat cgcttctctc ggtgccaatg ctcgcctggt cgggctgacg
    54721 ggcattgacg atgcggcgcg tgcgctgagt aaatctctgg ccgacgtcaa cgtcaaatgc
    54781 gacttcgttt ctgtaccgac gcatccgact atcaccaaat tacgggtact ttcccgcaac
    54841 caacagctga tccgcctgga ttttgaagaa ggtttcgaag gtgttgatcc gcagccgctg
    54901 cacgagcgga ttaatcaggc gctgagttcg attggcgcgc tggtgctttc tgactacgcc
    54961 aaagatgcgc tggcaagcgt acagcagatg atccaactgg cgcgtaaagc gggcgttccg
    55021 gtgctgattg atccaaaagg taccgatttt gagcgctacc gcggcgctac gctgctgaca
    55081 ccgaatctct cggaatttga agctgttgtc ggtaaatgta agaccgaaga agagattgtt
    55141 gagcgcggca tgaaactgat tgccgattac gaactctcgg ctctgttagt gacccgttcc
    55201 gaacagggta tgtcgctgct gcaaccgggt aaagcgccgc tgcatatgcc aacccaggcg
    55261 caggaagtgt atgacgttac cggtgcgggc gacacggtga ttggcgtcct ggcggcaacg
    55321 ctggcagcgg gtaattcgct ggaagaagcc tgcttctttg ccaatgcggc ggctggcgtg
    55381 gtggtcggca aactgggaac ctccacggtt tcgccgatcg agctggaaaa tgcagtgcgt
    55441 gggcgcgcag ataccggctt tggtgtgatg accgaagagg aactgaagct tgctgtagcg
    55501 gcagcgcgta aacgcggcga aaaggtggta atgaccaacg gtgtctttga catcctgcac
    55561 gccgggcacg tctcctatct ggcaaatgct cgcaaactgg gtgaccgtct gattgtcgct
    55621 gtgaacagcg atgcctccac caaacggctg aaaggggatt cccgccccgt taacccgctc
    55681 gaacagcgta tgattgtgct gggcgcactg gaagcggtcg actgggtggt gtcgtttgaa
    55741 gaagacacgc cgcagcgctt gatcgccggg atcctgccag atttactggt gaagggcggc
    55801 gactataaac cagaagagat cgccgggagt aaagaagtct gggccaacgg cggcgaagtg
    55861 ctggtgctca acttcgaaga cggttgctcg accactaaca tcatcaagaa gatccaacag
    55921 gataaaaaag gctaaccgga aagcggttca cagatctcgc ttcgtagcag gcaggcctat
    55981 tgacgctgtt gttttttcaa cctaaagtaa aggaacaagg gtaagggagg atttctcccc
    56041 cctctgatca gttgttagta agtcgggaaa cttaacagta acaacacaac cagtatgatg
    56101 acgaacttca tcataaccct ttccttctat aaggcccctt cctcgggagg ggctttcccg
    56161 tttcagcccc ttgctgactc cccgattgtt gatgagcgcc ggaccccgcg caacgctttc
    56221 cctgaagcag ttttgccttc cccgagcggt cacgcaaaac gctgcaacgt cctgagcatt
    56281 tgcacctttt agcggaacgc agatataaaa aatccccggc tttttgggcc agggatatgt
    56341 gttaatgaaa ccttcttgac gctgattttt tttcaaccta aagtaaagga acaagggtga
    56401 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgattaacaa
    56461 cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg ccccttccta
    56521 gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg agcgccggac
    56581 ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg caaaacgttg
    56641 caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc cccggctttt
    56701 tgtgacaggg acgttcatag ctgaaaattt attctacgtt ttcggcgacg ggagttgttg
    56761 aggttaaggg tgaagtcaat gccgccagtg agccgcctga aacgccaatt tcattgagca
    56821 aggagtcaat cagcggtgcc tgtgtgcggt aagagagagc ggctgataat gcttgttccg
    56881 ccaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg ccacgattca
    56941 gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca acggattttt
    57001 ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg gtttgttcat
    57061 cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc gccagaccct
    57121 ttttacgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct gcggcttctt
    57181 tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc tgcgcggcag
    57241 cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg gtttctacgt
    57301 tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct gcctgggact
    57361 gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt tcggtgacct
    57421 gctgttgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt cttgagcgga
    57481 cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga attcgcgtct
    57541 gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc tgttcggcag
    57601 tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt tgttcaatct
    57661 ccaacttgcg cgaaagtgca tcacggtttt tctcacgcac cgcaacttct acatcctgtt
    57721 caacttcgtt acgttcgcga cgacggcgct ccgtctcctg agtcagtttg gttaaacctt
    57781 cggcgtcaaa ggcattgttc ggattgaaat gttctttcga ggtctggtta aagttggtaa
    57841 gtgaaacgct ttccagctca agaccgtttt tcgacaggtc ttctgcgaca gtattttgta
    57901 ctccctgcac gaagttctcg cgggtatcct gtaactcatg catggtcatt tgtgcagctg
    57961 ttgcacggag ggcatcgaca aatttatctt caaccaacat acgtaagtct tctggtgata
    58021 gcgtgcgttg ccccaacgtc tgcgccgcag tggcgatgcc ttctacagac ggttttaccc
    58081 gtacaaagaa agcgactact acatcgacac gcatacgatc tttcgtaatc aggctatcaa
    58141 tggttgagcg gctgacttcc agcttcagag tattcatatt gatggggatt atttcatgaa
    58201 agatcggcat cacgattgcg ccaccgctca tcaccacttt ttgcccacct aaaccagtac
    58261 gaacaaaagc ttgctctgcc gaagcgcgac gatagagcct ggcgaaaata attccaataa
    58321 taaacagaat gcatacggca ataatcgcgg taaacatcca ggagggcaca gaattaacaa
    58381 tatcatccat tattaaattt ccttttgatt aataaattac aatatttgtg gccagggatt
    58441 gttttccgcc aaatatcgcg ttgcagaaag tcggcaaata attaacactt tgtctccttt
    58501 agtgaaaaat tttccttctt caggctccag tagtaaataa tgtatttggc caaattgatc
    58561 ggtgagtttt ccttcgcagg gattacctga agtggcttga tgaccggtaa ttaatgccat
    58621 gctaccaata tattcttctt ctgtaatagc tgaactatgg tcgcgtggaa tccagggcgc
    58681 aactatttta ccggtataat gcaccgcaat aatcgtaaac agtaaactga caggaaccac
    58741 gaacaggttc gacagtggtg attgccagac catgatgcag gcatgctgca acaggatgcc
    58801 aataagacca aaaaaaccag ccagcaagca gagaacgacg agagctggca atcttccgat
    58861 attgagataa tgaagtgcct ggctaatatg acctgtggtt atggaatcat aatggtcaag
    58921 atgtgcatca agagcaccgg agagcatgtg accgcagata agcgctaaaa tttctaaaag
    58981 gccgatcaac agtacaaagg aaatagcgaa aagataaggg gtattatagt cggcgaataa
    59041 aatcatatgt cactccaatg tccatttagt gacgcaatat ttactttggg atagtttcct
    59101 tgcttacatt tatctttgtt aataataaca catgttagag aagttctgga agagaaagtt
    59161 ttttaatatg gttttttatt tttcactaaa aagtgtgatc ggggacaata tatttacgca
    59221 cgttatgttt aaaggcacta cactgattgg ggaatactga aatcagaaaa agcatatggc
    59281 cttaaaaggt atgcgataac cggaggatgt gcttatggat catagtctta attctttaaa
    59341 taatttcgat ttcctggcgc gtagttttgc cagaatgcac gcagaaggtc gcccggtcga
    59401 tattcttgcc gttactggta acatggatga agaacataga acctggtttt gcgcacgtta
    59461 tgcctggtat tgtcaacaga tgatgcaggc aagagagctg gagttagagc actgatataa
    59521 cgggcctgat ggcccgtttt agtgtttgta ttagatgcta gttataggtg attttgagga
    59581 tgacgtcttt actaaacggt ccctcaacaa cagtctctgc cggattcgca ctaagttctg
    59641 ccctgaatgg tgcggtgacc gaaggtgcac tagacgatga aaaagaagca aattgtgaga
    59701 agcggttaaa ttccgttgct gttccatctt tatgaaatat cttcaatagc agtccattac
    59761 ccatatccag atggctataa tcactcaaaa tatccgtagt gaaaaagctc cctaaaatat
    59821 tgaattgctc agtacaggcc gcaccaggat cttttgtcat gactacacta aacgtctcac
    59881 taacgctggt atttttaatt gttcgggaat ttattttacc aaagtcaaca acgccatcag
    59941 ctggttccag tgcgacatta acaaagcagt caaggacatg aatattattt aatccacgaa
    60001 tcctgaaggt caaatttttg tctacttcta tagctcctgc gccactcata cctttaaact
    60061 ctagagcgat atattcatca ggtaacacca ggtttttatt aactctattg gcattttttg
    60121 ctaatttaat atagagttgt acgcgagctg gaaaggttac tgataattga ggctcaggac
    60181 ctccactaca gacagcgggg tttttatatt gattattaaa ggtacccgct ccttttatat
    60241 caatgcattc tggtgctgcg atagcattag gggcagtcga atcataatca actccattgt
    60301 atgttacgcc aaaaatatag gagttctgat tataaaagtc ctgaccgcct ggtaaaaaca
    60361 tcattatatt agtccaggct gatgggtatt cttctccagg tgatggggca ggtaatgcat
    60421 tattacagaa gacattcata aaatactcac tgctttgcca gatcttatca ccttttttac
    60481 cattactggg gatagaaatt cttggtacgt aaccaatgac tacggtattt cccccttcat
    60541 gttcagcata gcagtccatt gcccatacag gtaatgcact aaaacctata ataaatataa
    60601 ttacaattaa taagtagcgc atattattta tctcgtttgg ctacggaaac attacaagag
    60661 ttattaaccg tcgggcagtt tatttcataa aaacttaatc caccaaaatc attgatataa
    60721 ccaacctgaa aagctgacgg aacattttta aactgcgtat ctgcatggct ataaggggtt
    60781 accattaaac tttgcgtttg tggcaacatt gtgattccgt ttgttccgat ataacccagc
    60841 gtaacgtgat aaggcatctt attgttaaag cggatcgaac catcatttct acggatgatt
    60901 tcaacttttt cgtaagacat taccccatca tcttctaatt caatagcttt tggtcgccag
    60961 aatagtttca atgcgtgctg catagtgact tgcatcacat ttttttatct gtttttggcg
    61021 gtatttctcg cacgttaaaa tagaagagcg tttctctgtc attaggcagt tgagcaattt
    61081 tttcctgact tatcattctt atttgaattt gctcactggg atttaaacgt accatagggg
    61141 gaaccgtgac aataaagtca cgtgatttat tgcctttcgt atcttctatc catgtttgcg
    61201 ccagataggg tagatttttg ctttgattag taagttttaa tgcttcccct ttatctgact
    61261 cattcattat gatacgggta cggtccggga tgatggcacc ccaggcaatg ctgctcatgc
    61321 aggcgagtaa tactcctgct gcaatgagat tctttgtgtt taagtagcgc attagtttca
    61381 tcctgaatta attttggcta atacacggta aaataaccgt tccggtggtt aagttgctgc
    61441 tattgggtgg ttgaataaaa caggatttat caccccagcg taaagtaagt ttgctgtcat
    61501 cctgaatgcc gttgaggtat acaaagcagc catcagcaac cagtcctaat tctttatggc
    61561 tggttttatc ttttacgcta atccccagtg gtgggtgact accatctgcc aggcgaataa
    61621 cccccatgat ttgatagccc ttgcgggtat caagtttggc gtagccaata gcaccttcgg
    61681 ttaaggtggt ggtgataacc gagttttcga tatccacatt ttctggaaga tttcgggtat
    61741 caacgcttaa tgatgttgtt atgtaactgc tgactgatgg aataactccg atgccatacc
    61801 gattagttac cgcgcgttta ttgttcaacg gaatatcttc tgcaccatct gcgtcgatca
    61861 taaaacgcga gtcagcacta ccactatagt catggaaagc ggctccgtga cgagtcgccg
    61921 tgaatgaacc attccaactg gcattcagag aataataact gtctcgctgt gatgtcccgg
    61981 agagatagag acgaccgttc tcggtattat gctgataact ggcgcgtagt gacgctttca
    62041 tatctttgaa ttcatcattg tcgcctgaag cagaaacgct ccaggaatta tttcgatcgg
    62101 aagagtcata ccacgaagca gtatgcgaaa tcttgttatc ctgatttcgt tgcataccat
    62161 agctcaatgt tctactggtt ccccagggaa tcgaaatatt taggtacagt tgatcctggt
    62221 tgtcttcttc ccagtttatt cggctaaatg ttagagaagt ggagacgttt tttaatggcc
    62281 cgatatcaaa atcgcggcta agtgataatg aataattcac attactgctg gcgtcccagt
    62341 aagtattacg agccaaactt acgtatgtat ttaaccttaa tgattcaaga tactggttaa
    62401 acgtgacaat ataactctct ttttcattct gataattcgt gtaatggtta gtgtcattga
    62461 tatattcatt cattgtcaca aagtttttat cagagaaacg gtaaccagcg aaagtgagct
    62521 ggctaccggt agattcaaaa cgttttgaat agttagcgcg gtagctataa cccgtttctt
    62581 tatcctgatt atgcaactga gcatcagatc gtgtgacatc aaaagataat gaacccaatg
    62641 agttaaggtt aaagccaacg ccggcagcca gagattgata atcacggttt gttaaaactg
    62701 aaccaccata cagggataca ttgttcagcc aaccccatga gaattcacca gtccagaaga
    62761 agggttgttg taaggaatca ccaccaacgc tggtcggttt acccatagca gtggtgtagc
    62821 gaatctgccc tttacgcgtc agataaggaa tactattaga tgcaacttgc cattgcgtga
    62881 cttgcccgtc ctcttcttca accttgacgt ccagttgccc ctgtaatgta ttgaataaat
    62941 tagtaatagc aaatgggcca ggaggaacat tttcttgata aataatacgg ttgttctgtg
    63001 agacagtgac ctttgcattc gtttgtgcaa cacccgtgat ttgcggtgcg taaccacgta
    63061 gatcaggcgg caacatattc tcatcgcttt tcaacgaaac gccagaaaaa tggaaagagt
    63121 cgaaaataga ggattcagta tcgtattgac ctatagttag ttttgcatta attgctggga
    63181 ttgggcgaaa caaataaata cgcggaaaat ccaggttgtt cgcctggctt ttgccattgt
    63241 taaatgactg atcgtactgg taatcagtac gcaggcgcca ggccccaaga ttaaccccag
    63301 cctgcccata ggaacttata ttttgtgaat tatcgccctg atgtggtgca taatgagagg
    63361 catataaatt gtagtcaaga aatgccccag caataccatg atcccaaagt tcaggaggcg
    63421 tccagtttgc atctttgtat tttaaccatg cctgaggtgc agatatggat aattgcattt
    63481 tccctttatc gagataagtt gtaatttgtt tttctttttc gatagggtaa caaccatcga
    63541 tcaggttttg ctgagcctct tcaataatct catcagtaaa gcctaataat gttaattgct
    63601 ctggagtcag acaaactgca gtttggttat caattaatac ccattgcagg tccagatgga
    63661 ttggcgtcct tttattatta ataagagcgt ttaatgagta tttacctacg ggtaattggc
    63721 catcagtttc aaattgggaa aggttaacgt tttcacgatc ttcggcttcg attaaatctt
    63781 tgttgaattc aacggcataa gctgacgcac aaccaatgat gagaacggat aatgaacaat
    63841 aaatattttt aatcaattcg cttattgtgg ttaattttaa ttttttatac atctgttcca
    63901 cttatcatta atccgttaat gatgtggccg ggcgaacccg gcctaatgtg attactcgta
    63961 agcaatctta tagtcagcct gagcgtaaac gttgcccggg gtagcatctt tgcttgggga
    64021 ttccatacga gctttgaagt tcagttcctg atagctgtta gtattgttga atgtaattac
    64081 taccggagta cctaaagtca cttcgccgct ctggctatcc agaatacgta cgccaacgcc
    64141 tgtggcacca ccgatttcag tttctttggt gtttttcagc aggtcagtct gacccgtcat
    64201 agtattcgct gtggtgaaag taaccttcgc ttttgaataa gtggttccac cctggctcaa
    64261 catgcagtct tgcaggtgaa tagtgaaagg ctcagccaaa gatttctggt cgcttttcag
    64321 aacggtatct gctacttcac ccagatttac agttaagtct tcgtcacctg gtttaattga
    64381 acatggagca gagataactt cacctttaaa tttaacaatg ccgtgacctt cgtcagcaaa
    64441 tacgttattg gaaagaatca ttgctacgcc agcaacaaga agtgactttt taaaagcaga
    64501 cataatattc ctttat
//
LOCUS       DAZGTR010000014        63589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGTR010000014 DAZGTR010000000
VERSION     DAZGTR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.014"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..1091
                     /gene="hemB"
                     /locus_tag="ACHLCY_001014"
     CDS             117..1091
                     /gene="hemB"
                     /locus_tag="ACHLCY_001014"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP0368322.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDN
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1196..2047)
                     /gene="tauD"
                     /locus_tag="ACHLCY_001015"
     CDS             complement(1196..2047)
                     /gene="tauD"
                     /locus_tag="ACHLCY_001015"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP0368323.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTIAPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2044..2871)
                     /gene="tauC"
                     /locus_tag="ACHLCY_001016"
                     /pseudo
     CDS             complement(2044..2871)
                     /gene="tauC"
                     /locus_tag="ACHLCY_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
     gene            complement(2868..3635)
                     /gene="tauB"
                     /locus_tag="ACHLCY_001017"
     CDS             complement(2868..3635)
                     /gene="tauB"
                     /locus_tag="ACHLCY_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP0368324.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3648..4610)
                     /gene="tauA"
                     /locus_tag="ACHLCY_001018"
     CDS             complement(3648..4610)
                     /gene="tauA"
                     /locus_tag="ACHLCY_001018"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0368325.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            5226..5897
                     /locus_tag="ACHLCY_001019"
     CDS             5226..5897
                     /locus_tag="ACHLCY_001019"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP0368326.1"
                     /translation="MDKVLDSALPSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            5907..7103
                     /gene="yaiP"
                     /locus_tag="ACHLCY_001020"
     CDS             5907..7103
                     /gene="yaiP"
                     /locus_tag="ACHLCY_001020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP0368327.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDHFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            7081..7662
                     /locus_tag="ACHLCY_001021"
     CDS             7081..7662
                     /locus_tag="ACHLCY_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0368328.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            7664..8437
                     /gene="yaiO"
                     /locus_tag="ACHLCY_001022"
     CDS             7664..8437
                     /gene="yaiO"
                     /locus_tag="ACHLCY_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP0368329.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQNFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            8621..8896
                     /gene="frmR"
                     /locus_tag="ACHLCY_001023"
     CDS             8621..8896
                     /gene="frmR"
                     /locus_tag="ACHLCY_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP0368330.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            8931..10040
                     /gene="frmA"
                     /locus_tag="ACHLCY_001024"
     CDS             8931..10040
                     /gene="frmA"
                     /locus_tag="ACHLCY_001024"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP0368331.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            10133..10966
                     /gene="fghA"
                     /locus_tag="ACHLCY_001025"
     CDS             10133..10966
                     /gene="fghA"
                     /locus_tag="ACHLCY_001025"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0368332.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(11091..11630)
                     /gene="yaiL"
                     /locus_tag="ACHLCY_001026"
     CDS             complement(11091..11630)
                     /gene="yaiL"
                     /locus_tag="ACHLCY_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP0368333.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(11732..12943)
                     /gene="mhpT"
                     /locus_tag="ACHLCY_001027"
     CDS             complement(11732..12943)
                     /gene="mhpT"
                     /locus_tag="ACHLCY_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP0368334.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGDKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASALGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(13218..14231)
                     /gene="mhpE"
                     /locus_tag="ACHLCY_001028"
     CDS             complement(13218..14231)
                     /gene="mhpE"
                     /locus_tag="ACHLCY_001028"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP0368335.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(14228..15178)
                     /gene="mhpF"
                     /locus_tag="ACHLCY_001029"
     CDS             complement(14228..15178)
                     /gene="mhpF"
                     /locus_tag="ACHLCY_001029"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP0368336.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(15175..15984)
                     /gene="mhpD"
                     /locus_tag="ACHLCY_001030"
     CDS             complement(15175..15984)
                     /gene="mhpD"
                     /locus_tag="ACHLCY_001030"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP0368337.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(15994..16860)
                     /gene="mhpC"
                     /locus_tag="ACHLCY_001031"
     CDS             complement(15994..16860)
                     /gene="mhpC"
                     /locus_tag="ACHLCY_001031"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP0368338.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(16878..17822)
                     /gene="mhpB"
                     /locus_tag="ACHLCY_001032"
     CDS             complement(16878..17822)
                     /gene="mhpB"
                     /locus_tag="ACHLCY_001032"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP0368339.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(17824..19488)
                     /gene="mhpA"
                     /locus_tag="ACHLCY_001033"
     CDS             complement(17824..19488)
                     /gene="mhpA"
                     /locus_tag="ACHLCY_001033"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP0368340.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            19679..20512
                     /gene="mhpR"
                     /locus_tag="ACHLCY_001034"
     CDS             19679..20512
                     /gene="mhpR"
                     /locus_tag="ACHLCY_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP0368341.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            20589..21671
                     /gene="lacI"
                     /locus_tag="ACHLCY_001035"
     CDS             20589..21671
                     /gene="lacI"
                     /locus_tag="ACHLCY_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP0368342.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            21794..24868
                     /gene="lacZ"
                     /locus_tag="ACHLCY_001036"
     CDS             21794..24868
                     /gene="lacZ"
                     /locus_tag="ACHLCY_001036"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP0368343.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVIPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTFATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGELRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWLAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKYQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPGLPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPH
                     LTTSEMDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            24920..26173
                     /gene="lacY"
                     /locus_tag="ACHLCY_001037"
     CDS             24920..26173
                     /gene="lacY"
                     /locus_tag="ACHLCY_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP0368344.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(26252..27433)
                     /gene="cynX"
                     /locus_tag="ACHLCY_001038"
     CDS             complement(26252..27433)
                     /gene="cynX"
                     /locus_tag="ACHLCY_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP0368345.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVSWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(27439..27909)
                     /gene="cynS"
                     /locus_tag="ACHLCY_001039"
     CDS             complement(27439..27909)
                     /gene="cynS"
                     /locus_tag="ACHLCY_001039"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP0368346.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(27940..28599)
                     /gene="cynT"
                     /locus_tag="ACHLCY_001040"
     CDS             complement(27940..28599)
                     /gene="cynT"
                     /locus_tag="ACHLCY_001040"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP0368347.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            28709..29608
                     /gene="cynR"
                     /locus_tag="ACHLCY_001041"
     CDS             28709..29608
                     /gene="cynR"
                     /locus_tag="ACHLCY_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP0368348.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(29648..30931)
                     /gene="codA"
                     /locus_tag="ACHLCY_001042"
     CDS             complement(29648..30931)
                     /gene="codA"
                     /locus_tag="ACHLCY_001042"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP0368349.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(30921..32180)
                     /gene="codB"
                     /locus_tag="ACHLCY_001043"
     CDS             complement(30921..32180)
                     /gene="codB"
                     /locus_tag="ACHLCY_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP0368350.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(32519..34405)
                     /gene="prpE"
                     /locus_tag="ACHLCY_001044"
     CDS             complement(32519..34405)
                     /gene="prpE"
                     /locus_tag="ACHLCY_001044"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP0368351.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(34445..35896)
                     /gene="prpD"
                     /locus_tag="ACHLCY_001045"
     CDS             complement(34445..35896)
                     /gene="prpD"
                     /locus_tag="ACHLCY_001045"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP0368352.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(35930..37099)
                     /gene="prpC"
                     /locus_tag="ACHLCY_001046"
     CDS             complement(35930..37099)
                     /gene="prpC"
                     /locus_tag="ACHLCY_001046"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP0368353.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIANRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(37144..38034)
                     /gene="prpB"
                     /locus_tag="ACHLCY_001047"
     CDS             complement(37144..38034)
                     /gene="prpB"
                     /locus_tag="ACHLCY_001047"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP0368354.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTIDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            38273..39859
                     /gene="prpR"
                     /locus_tag="ACHLCY_001048"
     CDS             38273..39859
                     /gene="prpR"
                     /locus_tag="ACHLCY_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP0368355.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIHKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRLGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(39957..40232)
                     /gene="yahO"
                     /locus_tag="ACHLCY_001049"
     CDS             complement(39957..40232)
                     /gene="yahO"
                     /locus_tag="ACHLCY_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP0368356.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            40379..41050
                     /gene="yahN"
                     /locus_tag="ACHLCY_001050"
     CDS             40379..41050
                     /gene="yahN"
                     /locus_tag="ACHLCY_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP0368357.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(41067..41312)
                     /gene="yahM"
                     /locus_tag="ACHLCY_001051"
     CDS             complement(41067..41312)
                     /gene="yahM"
                     /locus_tag="ACHLCY_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP0368358.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGQSSPMVGIRQIRLIT"
     gene            complement(41724..42539)
                     /gene="yahL"
                     /locus_tag="ACHLCY_001052"
     CDS             complement(41724..42539)
                     /gene="yahL"
                     /locus_tag="ACHLCY_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP0368359.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(42782..43831)
                     /gene="yahK"
                     /locus_tag="ACHLCY_001053"
     CDS             complement(42782..43831)
                     /gene="yahK"
                     /locus_tag="ACHLCY_001053"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP0368360.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAEPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(43918..44874)
                     /locus_tag="ACHLCY_001054"
     CDS             complement(43918..44874)
                     /locus_tag="ACHLCY_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0368361.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(44871..45842)
                     /locus_tag="ACHLCY_001055"
     CDS             complement(44871..45842)
                     /locus_tag="ACHLCY_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0368362.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTAAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(45835..47319)
                     /locus_tag="ACHLCY_001056"
     CDS             complement(45835..47319)
                     /locus_tag="ACHLCY_001056"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0368363.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(47368..48351)
                     /locus_tag="ACHLCY_001057"
     CDS             complement(47368..48351)
                     /locus_tag="ACHLCY_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0368364.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGNAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(48605..49015)
                     /locus_tag="ACHLCY_001058"
     CDS             complement(48605..49015)
                     /locus_tag="ACHLCY_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0368365.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(49314..50696)
                     /gene="yahJ"
                     /locus_tag="ACHLCY_001059"
     CDS             complement(49314..50696)
                     /gene="yahJ"
                     /locus_tag="ACHLCY_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0368366.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(50706..51656)
                     /gene="yahI"
                     /locus_tag="ACHLCY_001060"
     CDS             complement(50706..51656)
                     /gene="yahI"
                     /locus_tag="ACHLCY_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP0368367.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDVGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(51800..53218)
                     /gene="yahG"
                     /locus_tag="ACHLCY_001061"
     CDS             complement(51800..53218)
                     /gene="yahG"
                     /locus_tag="ACHLCY_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0368368.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(53218..54765)
                     /gene="fdrA"
                     /locus_tag="ACHLCY_001062"
     CDS             complement(53218..54765)
                     /gene="fdrA"
                     /locus_tag="ACHLCY_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0368369.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIPPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(54755..55618)
                     /gene="yahE"
                     /locus_tag="ACHLCY_001063"
     CDS             complement(54755..55618)
                     /gene="yahE"
                     /locus_tag="ACHLCY_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0368370.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGQNETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(55658..56263)
                     /gene="yahD"
                     /locus_tag="ACHLCY_001064"
     CDS             complement(55658..56263)
                     /gene="yahD"
                     /locus_tag="ACHLCY_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0368371.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCFALGVDINTCDRQGKTTIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            56521..57018
                     /gene="yahC"
                     /locus_tag="ACHLCY_001065"
     CDS             56521..57018
                     /gene="yahC"
                     /locus_tag="ACHLCY_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0368372.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            57110..58042
                     /gene="yahB"
                     /locus_tag="ACHLCY_001066"
     CDS             57110..58042
                     /gene="yahB"
                     /locus_tag="ACHLCY_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0368373.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(58084..59172)
                     /gene="pdeL"
                     /locus_tag="ACHLCY_001067"
     CDS             complement(58084..59172)
                     /gene="pdeL"
                     /locus_tag="ACHLCY_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0368374.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(59314..63297)
                     /gene="ehaA"
                     /locus_tag="ACHLCY_001068"
     CDS             complement(59314..63297)
                     /gene="ehaA"
                     /locus_tag="ACHLCY_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP0368375.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDD
                     GNSAIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSTEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        15128 a        17339 c        15829 g        15293 t
ORIGIN      
        1 atgtcctggt ctctctttca ttctcttctt gtcaacatca cgacaacttt cgtaaaacac
       61 tcctgccccg cttcaggtat actgtgcacc ttaattccac aaacatcagg cagaccatga
      121 cagacttaat ccaacgccct cgtcgcctgc gcaaatctcc tgcgctgcgc gctatgtttg
      181 aagagacaac acttagcctt aacgacctgg tgttgccgat ctttgttgaa gaagaaattg
      241 acgacaacaa agccgttgaa gccatgccag gcgtgatgcg cattccagag aaacatctgg
      301 cacgcgaaat tgaacgcatc gccaacgccg gtatccgttc cgtaatgact tttggcatct
      361 ctcaccatac cgatgaaacc ggcagcgacg cctggcggga agatggactg gtggcgcgta
      421 tgtcgcgcat ctgcaagcag actgtgccag aaatgatcgt catgtcagac acttgtttct
      481 gtgaatacac ttctcacggt cactgcggtg tgctgtgcga gcatggcgtc gacaacgacg
      541 caactctgga aaatttaggc aagcaagccg tggttgcagc tgctgcaggc gcagacttca
      601 tcgccccttc cgccgcgatg gatggccagg tacaggctat tcgccaggcg ctggacgctg
      661 caggctttaa agatacggcg attatgtcgt attcgaccaa gttcgcctct tccttttatg
      721 gtccgttccg tgaagctgcc ggaagcgcat taaaaggcga ccgcaaaagc tatcagatga
      781 acccaatgaa ccgtcgcgag gcgattcgtg aatcactgct ggatgaagcc cagggcgcag
      841 actgcctgat ggttaaacct gctggagcat acctcgacat cgtgcgtgag ctgcgtgaac
      901 gtactgaatt gccgattggc gcgtatcagg tgagcggtga gtacgcgatg attaagttcg
      961 ccgcgctggc gggtgctata gatgaagaga aagtcgtgct cgaaagctta ggttcgatta
     1021 agcgtgcggg tgcggatctg attttcagct actttgcgct ggatttggct gagaagaaga
     1081 ttctgcgtta atttttttcc tctcagaaag ggggagttaa cctgttgaaa gtacggtgaa
     1141 cgccccctca ccctaaccct ctccccaagg gggcgaggga accgtcccac tcgtatcacc
     1201 ccgcccgata aaacggttta tccccaagga tcgtcgcccg atgcataatc cgccgctgtg
     1261 gcaggtaatc ggcattggca tagtgctgag tcacgcggtt atcccaaatc gcaatatcat
     1321 tcggttgcca gcgccaacgc acctgaaact ccggtttagt gatatgggcg aataaaaagc
     1381 ctaacaaggc ttcgctctct ttctcgctga catcgacaat tcgcgtagta aagccttcat
     1441 tcacaaacag cgcctgttta ccgctcaccg gatgcgttcg caccaccgga tgtagcaacg
     1501 gaggattttt cgcgaccgcc tcgcgccagc gttgatgttc ctcctcggtt ttgcggtatt
     1561 tgtattccgg gaacgattta cggaaatcat gctccgcacg cagcccgctc agcagctggc
     1621 ggaagggaac agagagcgcc tcataggccg caataccgct ggtccagagc gtatcaccgc
     1681 cggtcgaagg taactcttta gctgccagaa tcgcccctgc gggtggcgtt tcaataaatg
     1741 tcacatcggt atgccagttg tcgttatccg gcggattatc gttatgggta tccagcacga
     1801 tgatctcgtc aaccccttcg gcatgagggt aaacagggtg aatatgcagt tcgccaaaac
     1861 gctgggccag cgcgcgttgc tgctgcggcg caatagtttg atcgcgcaga aacaccacct
     1921 gatggcgcag caccgcatgg taaagctgtt caaactgatt atcgcttaac gggcgcgtca
     1981 ggtcggcacc cgaaatttgt gcgccgatat acggccccag cggggtaatg ctcagacgtt
     2041 cactcattgt acttctccat gccagggcgt cagacggcgc tgtaacgcgc gcaaacccag
     2101 ttctaaaaga aaggcgataa tcgcaatcac cgcgatcccc gccagcacca cgtcagtcgc
     2161 gagaaattca cccgctgact gaaccataaa tcctaaaccg cgcgtcgcgg caatcagctc
     2221 cgccgctacc agcgtagacc accccacacc cagaccaatg cgtaatccgg tgaggatttc
     2281 cggcagcgcg ccgggcaaaa tgacaaacca cagcacctgc gcacggctgg cacccagcga
     2341 ctgggcggca cgaatgcgaa cctgctgcgc gcttttcacc cccgccagcg ccgacatcgc
     2401 caccggtgca aaaatcgcta aatagatcag taagatcttc gaggtttcac caataccaaa
     2461 ccagatcacc atcagcggca aataagccag cggcggcacc ggacgataaa gttcgattac
     2521 cggatccaga atgccgcgca ccgtcgggct tagccccatg gcgatcccga ccggaatacc
     2581 gatcaccacc gccgcgagta gcgccagcac aatgcgcgtc aggctggctg ccagatgctg
     2641 ccacagcgtg gcgtccataa atccctgcgg tccggcaatg gtgagcagtt ttgccagtac
     2701 ttgttgcggc ggcggcaaaa acagcgggct aatcagttgc agcgccgcca ccgtccacca
     2761 tacagtgagt aaaaccgcta acgtgccaat gcttaaggtc acctgacgct agagcggcca
     2821 gcgccatttc agccgccgcg aatgcagttt ttcattaatg agcacactca tgagaacgcc
     2881 tcccgttgct caaatacgcg gcttaaaaca tattcgcgca tggcgatgaa ttgtggatcg
     2941 gacttgatgc tgcggctcga ctctcccgca acaaagcggc gagcaaagtt gagcggcagc
     3001 cgctccagca cgcggccagg gccgggtgaa agcagaacca gttcagtcgc cataaacacc
     3061 gcttcttcta tatcgtgggt aatcaacagc acctgcttgc ccgtctcctg ccagagtttc
     3121 agcagcaggg tttgcatctg gtcgcgggtg aaggcgtcca gcgcaccaaa cggctcgtcg
     3181 agtaataaca gctggggatt cgccgccagc gcgcgggcaa tccccacccg ctgacgttga
     3241 ccaccggaaa gctgccagat atagcgtttt tctgcgcctt ccagccccac ttttttcagc
     3301 atctggtgcg cgatttccag tcgctgcatt ttctctatac ctgccaattg caggccgaac
     3361 gccacgttgt cctgtacatt gcgccacggt agcaaccctt cattctgaaa aaccaccccg
     3421 cgctctgctc ccggtccctc aatacgctta cccgccagtt gaatgctgcc atgctgataa
     3481 ggcacaaaac cggcaatcag attcagcagg gtggttttac cgcagccgga cggccccagc
     3541 accaccagta gctcgccgct ttccagcgtc aggttgatat cttccagtgc cggtttgccg
     3601 ccataatcgg cgtaaagatg agagatttgc agcatctgcg cctcctttta ttgcacgaag
     3661 cgcgaggtaa cgtactggct gtaatcattc gctacagccg ggaccttgcc ctgctctttc
     3721 aaaaactgcg cggtgtcgat gatcgctttg ttcaccggtc cggtcagttc tgccgtttgt
     3781 tgctgcggcg tcagataggt attccccttc accagccccg gaacgtcacc ttccggcacg
     3841 ccgcttaaac gcgccagttt gctgatgttt tccggctgtt tcagccacgc gtctgggtta
     3901 gcaatgtacg gttgctgagc atcgatggcg cttttagcga acgctttcac gacctcagga
     3961 tgtttctcgg caaaatcttt gcgcaccacc cagacgtcca gcgttggcgc accccactgc
     4021 ccgacctgtt cagaatcggt cagcaccttg ccgtcttttt ccagggcgtt aaccgccggt
     4081 gcccagacat aagcaccatc aatatctccc cgctgccagg cagcgataat cgcgggcggc
     4141 tgcaggttca caatctccac ttgcccgggt ttaatgcccc agtgtttcag cgccgccagc
     4201 aggctgtagt gggtggtaga gataaacggt acggcgatgc gtttgccaat cagatcttcc
     4261 ggtttgctga tagttttctt taccaccagc gcttcggagt tacccagttt tgacgccagc
     4321 aagaaaactt caatcggcac ctgttggctg gctgcaaccg ctaacgggct ggaaccgagg
     4381 ttgccgattt gcacatcgcc agaagccagc gcccgcacga tgctggctcc gctgtcaaac
     4441 ttacgccagt ccacggttgc tccgctttct ttagcaaagg tgttgtcggc ctgagccact
     4501 ttcgccggtt cggctgaggt ttgatacgca acggtgacgt tcaccgcctg tgcctgaaaa
     4561 gcgatgaatg ccagtgcggc aagaagtgtg ttacgcgatg aaattgccat gattgtctgc
     4621 tcccctgtct tgttatgtga gcagtattca ggaataaaaa cattcattaa aagaattagt
     4681 cgttatcgca cagatgattt tattcttagc aaaaaaacgg caaaggaaag attaaatatg
     4741 caattactcc gttctaacaa ttgggaaaat tactacactt cattttagtg atgcataaac
     4801 aactacatta cgagatttat tacttcatta tttctatcaa tatcaataca tataataaaa
     4861 ctattatatt cgcattgtat atatcactgt atcattctca atcactcgca tattacattc
     4921 acataatgta tataaacaat acttgttcac agaacatatt ttttaaatgc aattaattga
     4981 caaactaaat cacatctaaa tacaatgtat aaaacaaaat aactatacat acaaaacata
     5041 caaacactta acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa
     5101 gcccttgctt ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc
     5161 agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc
     5221 attaagtgga taaggtttta gattcagccc tcccttcttc ggcaaataaa agaaagggta
     5281 tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc
     5341 gtcttgcgca aaaaggaatt tatatcgcag ccgtggtgat gactaccggc aactctggta
     5401 cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgcc
     5461 accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga
     5521 tttccgctct ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg
     5581 tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa
     5641 tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt
     5701 catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg
     5761 cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat cgcctgcgtg
     5821 cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc
     5881 ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt atgtctatag
     5941 caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta
     6001 ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc
     6061 tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata
     6121 ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccactttgtt gccgtcacgc
     6181 aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc
     6241 aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata
     6301 tgctggcaga aattgagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga
     6361 aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga
     6421 agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa
     6481 tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc
     6541 ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca
     6601 tcgtataccc acaggaatgc aacagcccgc gtgaggagtg gcgtcgctgg cggcgttgga
     6661 ttgtgggata cgcggtctgt atgcgcctgc ataaaagact tttatttagc cgcttcggta
     6721 tcttcagtat atttcctatg ctgttggttg tgctttatgg cgttgggatt tatctcacta
     6781 cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc
     6841 ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct
     6901 ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct
     6961 ggattattta tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca
     7021 cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa
     7081 ctgaaaaact atctgaagct taatgttaaa gagaaaaata ttcagattgc agaccaggta
     7141 attattgatg aaactgcagg tgaagttgtt atcggcgcga atacccgtat ttgtcatggt
     7201 gccgttattc agggtccggt agtgattggc gcaaactgcc tgataggtaa ttatgcgttt
     7261 attcgtcctg gcacaataat cagcaatggt gtaaaaattg gttttgccac cgaaattaaa
     7321 aatgcggtta ttgaagcgga agcaacgatt ggtccgcaat gttttattgc cgactcggta
     7381 gttgcaaacc aggcatattt gggcgcacaa gtacgtacca gtaatcatcg tctggatgaa
     7441 cagcctgtgt ctgttcgaac tccagaggga attatcgcta ccggatgcga taaattaggt
     7501 tgttatatcg ggcagcgttc acgccttggc gtacaagtta taattttgcc tgggcgaatt
     7561 atttccccga acacgcaact tggcccgcgc gtgattgtag aacgtaattt acctgccgga
     7621 acttattcac tccgacaaga acttatccgt acaggagatt aaaatgataa aacggacgtt
     7681 attagcggcg gccattttta gcgcattgcc cgcttatgcc gggttaactt ccattaccgc
     7741 gggctacgat tttaccgatt attctggcga tcatggcaac cgtaatttag cgtatgctga
     7801 actggtggcg aaagttgaaa acgcaacgct gctttttaat ctttcccagg ggcgtcgtga
     7861 ttatgaaact gaacatttca atgccactcg cggtcagggt gcggtctggt ataaatggaa
     7921 taactggctg acaacccgaa cgggtattgc ctttgcggat aatacgccgg tctttgcccg
     7981 ccagaatttt cgtcaggata ttaacctggc cctgttgcca aaaacgcttt tcacgaccgg
     8041 ttatcgctac actaaatatt acgatgatgt cgaagtcgat gcctggcaag gcggcgtatc
     8101 actctatact ggcccggtga tcaccagcta ccgctatacc cattatgact ccagcgatgc
     8161 aggtggtagt tatagcaata tgatttccgt gcgtctgaat gacccgcgcg gcactggtta
     8221 tacgcaacta tggctaagcc gcggaacagg cgcttacacc tatgactgga cgccagaaac
     8281 acgctacggc agcatgaaga gcgtcagtct gcaacgtatt caaccgctga ctgagcaact
     8341 taatctcggg ctgacggcag gtaaagtgtg gtacgacacc ccaaccgatg attataacgg
     8401 tctgcaactt gcagcccatc tgacctggaa attctgattc cttctgccgc ccgctatccg
     8461 gggcggcctt ccctgccgat tagccccccc tttcctcttt gttttccgac cacattcacc
     8521 ggataaattt tattctccag tgttatatac tatagggggg tatgcattga catatagcat
     8581 accccctata gtatattgcg tgcagatgat gaggtgcgaa atgcccagta ctccggaaga
     8641 gaagaaaaag gtccttactc gagttcgtcg tattcgaggg cagattgatg ctctggaacg
     8701 gtcgctggag ggtgatgccg aatgccgtgc catactccaa cagatcgctg ccgttcgggg
     8761 cgcggctaac gggctgatgg cagaagtgct tgaaagccat atccgggaaa cgtttgaccg
     8821 aaatgactgc tacagccgcg aagtcagcca atccgttgac gacactattg aactggttcg
     8881 agcctatctt aaatagctga atctattacc atattgagga agagcgagag atgaaatcac
     8941 gtgctgccgt tgcatttgct cccggtaaac cgctggaaat cgttgaaatt gacgttgcac
     9001 caccgaaaaa aggtgaagtg ctgattaaag tcacccatac cggcgtttgc cataccgacg
     9061 catttaccct ctccggggat gacccggaag gtgtattccc ggtggttctc ggtcacgaag
     9121 gggccggcgt tgtggttgaa gtcggtgaag gcgtaaccag cgtcaaacct ggcgaccatg
     9181 tgatcccgct ttacaccgcg gagtgcggcg agtgtgagtt ctgtcgttct ggcaaaacta
     9241 acctctgtgt tgcggttcgc gaaacccagg gtaaaggctt gatgccagac ggcaccaccc
     9301 gtttttctta caacgggcag ccgctttatc actacatggg gtgctctaca ttcagtgaat
     9361 acaccgtggt cgcggaagtg tctctggcca aaattaatcc agaagcaaac catgaacacg
     9421 tctgtctgct gggctgtggc gtgaccaccg gtattggcgc ggtgcacaac acagctaaag
     9481 tccagccagg tgattctgtt gccgtgtttg gtcttggcgc gattggtctg gcagtggttc
     9541 agggcgcgcg tcaggcgaaa gcgggacgga ttatcgctat cgataccaac ccgaagaaat
     9601 tcgatctggc gcgtcgcttc ggtgctaccg actgcattaa cccgaatgac tacgacaaac
     9661 cgattaaaga tgtcctgctg gatatcaaca aatggggtat cgaccatacc tttgaatgca
     9721 tcggtaacgt caacgtgatg cgtgcggcgc tggaaagtgc gcaccgcggc tggggtcagt
     9781 cggtgatcat cggggtggca ggttctggtc aggaaatctc cacccgtcca ttccagttgg
     9841 tcactggtcg cgtatggaaa ggttccgcgt ttggcggcgt gaaaggtcgt tcccagttac
     9901 cgggtatggt tgaagatgcg atgaaaggtg atatcgatct ggaaccgttt gtcacgcata
     9961 ccatgagcct tgatgaaatt aatgacgcct tcgacctgat gcatgaaggc aaatccattc
    10021 gaaccgtaat tcgttactga tttcccgcag gtataccccg tccacttcag acggggttct
    10081 taatactctc cctgggcagc cgtccggggg attaaccgtg agataatgac tgatggaact
    10141 cattgaaaaa catgccagct ttggcggctg gcaaaatgtg tatcggcatt attcccaatc
    10201 actgaaatgt gaaatgaatg tcggcgtcta tctcccacca aaagccgcga atgaaaaatt
    10261 gccggtgctg tactggcttt cgggcctgac ctgcaacgag cagaatttca ttactaaatc
    10321 ggggatgcag cgttacgcgg ctgaacacaa cattattgtt gttgcgccgg acaccagtcc
    10381 gcgaggcagt catgtcgcag atgccgaccg ttacgatctc gggcaaggtg ccgggtttta
    10441 cctgaacgca acgcaagcgc cgtggaatga acattacaaa atgtatgact atatccgcaa
    10501 cgagctgccg gatttagtga tgcaacattt tccggcaacg accagaaagt ctatctctgg
    10561 tcattctatg ggcgggctgg gcgcgctggt gctggcgtta cgtaacccag atgaatatgt
    10621 cagcgtctcg gcgttttcgc ccattgtctc cccatcgcaa gtaccgtggg gacagcaagc
    10681 ctttgccgca tatcttggtg aaaataaaga tgcctggttg aattacgatc cggtgagtct
    10741 tatttcacaa ggtcaacgcg ttgcggaaat catggttgat caggggttga gtgatgattt
    10801 ttacgcggaa cagctgcgga ctccaaatct tgaaaagatc tgccaggaga tgaatatcaa
    10861 gacgttaatc cgttatcacg agggttacga ccacagctat tattttgtct ccagttttat
    10921 tggcgagcat attgcctacc acgccaataa actgaatatg cgttgataat agtgcacgac
    10981 tgccggatgc ggcgtgaacg ccttatccgc ctacatttcg cccgcaggcc gtaggcatga
    11041 taagacgcgc atagcgtcgc atcaggcatc tgcgcacgac ttatctatgt ttaccacatc
    11101 aaatcatcag gcactttgaa gtcggcatat gggtcgtctt catcctgctc ttcggcgctg
    11161 agcgcgctgt gcaacactat actgctggca tcacgctgag ctattttatc ggcaacgctg
    11221 gcgggaataa tggcgtattc accttcgcta ttgttatcga ccaacaagcg agcaatcgcc
    11281 agacgaccat taatcaactg cgcctgtgtg agcttatcga caaaaatctt cttaatcaga
    11341 ttgccgtcag tgaagttaaa accaatatcg ccattggcaa tggtgattcg gttcatttca
    11401 atgagctgct tcacctgagc tttatattct ttcgccaacg cagcttgttt ttgttgctcg
    11461 ctaagctgtt tatcacgctc aagctgtgcc tttttatttt cttctaccgc cgcccgagct
    11521 tcacgcgcct gaacgcgcga ttttttcgcc gttctctcca ccttcgccgc ttttttgctg
    11581 gtgactaatc ctgctttgag caactgctct tgtaaggtaa gttttgccat ctttgtttcc
    11641 aaacccgctg aataattttt agcgagtata cctgtaaacg ccgtgggtgt attgggggca
    11701 actccctttc gcccctgctt aataaggcgc gtcaggcatc ggcacacggc tgcattcttg
    11761 atctccgact catcaaaata aacaccgcca acccagcaac aagaataccc agtgcagaag
    11821 cggccattac gccgaccgtg ccagtgccta atgccagcat tttcccggcc agtaacggac
    11881 cgctcatagc ccccagacgc cctaccgcca cggctgttcc cacacctgtt gcgcggatct
    11941 gcgaactgta aaacaacggt gccagggcat acaaaacgct ttgcccacct gtcgcaaaca
    12001 accccgcgac aaatcccgcc agcaacatac cgttaaatga cgacaccgtt ccaagtgcca
    12061 gcagcgaagc taacatgccg ctataaatca gtagcgacat ggtcactgga cgcagcttat
    12121 ccatcaatgc gcccaacatt aacgtcccgc ttgccgcacc catttgcagg gcaaacatca
    12181 cccctgccgc ctgcgatggc tggaatcctt gctccaccaa aagcagcggt agccagttga
    12241 tcaacatgta gaccaccagc agagtgaaga aataacacaa ccacagcagc agcgtcgcgg
    12301 ttgccgtttc tggcgcaaat aaggcacgca gtggtggcgc agcctgttta tcgccagcga
    12361 aaaccgccga ctccggcagc cagcgcatta atagcggcac cagaatcaac ggcaccacac
    12421 cacctaccca aaacaccgtt tgccatgcta agtttgcccc cgcgaaaccc agtgtcgccg
    12481 ccagcgccgc gccaatggga acaccgcaat acatcaggct cactgctgtc ccacgaaaac
    12541 gtggacccgc ggcttcagac gtcagggcga taagattcgg caacgccgcc cccagcccga
    12601 caccggtcat cagccgcgca aagaccagtg aggggaaatc ccaggcaatc gccgttgcca
    12661 gcgagaacaa accaaacagc gcaactgagc caatcaaaat gcgcttgcga ccataacggt
    12721 ccgccagcat tccgccaacc aacgcgccgg gtagcaaacc gagtattccg gcgctgaata
    12781 tccagcccat ttgcatttta tcgagtgcga aagcctgggc gatgccaccc gccgcaatgc
    12841 cagccgcctg aagatccagc ccttccatca gagcgaccaa aaaacaaagc ccgatagtca
    12901 gcatcaggcg ggatgaagat gatgaagggg tacgagtcga cataagatga acctcgttgc
    12961 gagtgcaggt gaatcgcgac tgatacattg ccagccgcgt aatcagaact atttaatgac
    13021 tgcctacggg cgaaatgtag gccggacaag acgtttatcg catccggcag tcgtgtgcag
    13081 atgcctgatg cgacgctaac gcgtcttatc aggcctacaa attctcacgc cacccgtagg
    13141 ccggataagg tgtttacgcc gcatccggca gtcgtgctgg gatgggcata caaaatgcac
    13201 gttctcggca gtcatcatta tttgttgttg cgcagatcca gcgccacgtc aacgatcata
    13261 tcctcctggc cgccaaccat ccggcgtttg cccagctcaa cgagaatatc caccgcactt
    13321 aagccataac gcgccgccgc cgtttcacag tgacgcagga agctcgagta aacaccagcg
    13381 tatcccagcg ccagcgtttc gcgatcgact cgtaccggtc gatcctgcaa cggacgcacc
    13441 aggtcgtcag cggcatccat taacgcatag agatcggtcc catgctgcca gcccagttta
    13501 tccgcggcgg caataaatac ttccagcggc gcgttacctg cgcccgcgcc cattcccgcg
    13561 aggctggcgt cgattcggtc gcagccctct tccaccgccg cgatagagtt cgccacgcca
    13621 agactcaggt tatggtgagc gtgcatgcca gtttgcgttt ctggtttcag ctctgctttc
    13681 agggcgcgga aacggtcacg gatatcgctc atgttcatcg caccgccaga atccaccaca
    13741 taaatacagg tcgcaccgta gccttccatc agctttgcct gctttgcgag attctccggc
    13801 gtggtcatat ggctcatcat cagaaaacca acggtgtcca ttccgagctc gcgggcatac
    13861 tgaatatgct gggcggaaac atcagcttcg gtacagtgcg ttgccacacg aaccacccgc
    13921 gcgccagcct gccaggcatt tttcagatcg tgaatagtgc cgattcctgg cagcaacaac
    13981 gtcgcgattt tggcgtgctt caccacatcc gccgctgctt caatccattc aaggtcgcta
    14041 tgtgcgccga aaccatagtt aaagctggaa ccctgcaaac cgtcgccatg ggccacttca
    14101 atcgaatcca cgcgggcatc gtccagtgct ttggcaatct ggcgaacgtt ttccagcgaa
    14161 tactgatgac gaatggcgtg cataccgtca cgcaacgtga cgtccgagat ataaagtttt
    14221 ttaccgttca tgccgcttct cctgccttgc gcgccagtga ctgggccatt ttttccgctg
    14281 tcgccagcgc actggaagtc ataatgtcga ggttgcccgc ataggcaggc agataatgcg
    14341 ctgcgccttc gacttccagc cagaccgctg ttttcagtcc ggagaattgc cccacgcccg
    14401 gtaaattgac cggtttatcc tgcgggataa cttcaaactg cacgcgctgt ttcaggcgat
    14461 aacccggtac gtaagcctgc accgcctcag ccatttcatt gattgaggct tcgatatcat
    14521 cttgtgaagc ttcgtcgctc aatacataca ccgtgtcacg catcatcaac ggtggctctg
    14581 ctgggttaag cacaataatc gccttccctt ttgccgcgcc gcccaccact tcaatggctc
    14641 gggaagtggt ttccgtaaat tcatcgatat tggcacgcgt gccaggtccg gcagatttac
    14701 tggcgataga agcgataatt tcggcgtaat gaacacgcgc cacgcgtgaa actgccgcca
    14761 ccattggaat ggtggcctgg ccgccgcagg tgaccatgtt gacgttcagt tgatcgacgt
    14821 tcgcctcgag gttaaccacc ggcacgcagt aagggccgat ggcagcaggc gtcaggtcaa
    14881 ttaagcgaat atccggtttc gcttcgcgta aagcggcatc gtttttcaca tgcgcgcccg
    14941 cgctggtcgc atcaaataca atgtcgatat cagcaaattc aggcatgttc atcagcccga
    15001 tcaccccttc atgggtggtg gcgacgccca tacgtctggc acgcgccaga ccgtcggact
    15061 gaggatcaat gccgaccatc accgccatct ccagatgctg accgtgacgc aaaattttaa
    15121 tcatcagatc ggtgccaatg ttgccagaac cgataatggc gactttacgc ttactcatga
    15181 cagacttcct tttggggctg cgcttgaaaa tgtcgcagca actgaaccta tgccttcaat
    15241 atgggcttca aaacgatcgc ccgcattcac cgccaccatc gggcctaatg ccccggtaag
    15301 aatgatatct ccggcgcgca gcggttcacc cagactggcc attttgcgtg ccagccagac
    15361 ggcggcatta agcggatgtc ccaggcattc gctgccgcgc ccgctggaaa cctcttcgtt
    15421 attacgcgtc atcttcatgg cgcagttttt caggtctaac cccgccggac gttgcgccgg
    15481 accgccgatg acatacaccc cgcaggaagc gttatctgcc acggtatcga caaactgaat
    15541 cgaccagtcg cgaatgcggc tccccaccac ttccagcgcc ggaagtaccc attcaatggc
    15601 gttatacagt tcgtcgaagg tgatatcagt tgcgggtaaa tcgcggttca acaccagtgc
    15661 gatctccgct tcaatgcggg gttgcagaac acgggaaaaa ggaatgattt cgttatcgcc
    15721 ataacacatg tcggcaaata acgtcccaaa atccggttga tcaacgccca gttgttgttg
    15781 cactttcgga tgcgtcaggc ccactttacg ccctaccacg cgacgcccct gcacaacgtc
    15841 atattgcaca tttatgtgct gaatggcgta ggccgcttca gcgttatcga taccaatcag
    15901 atcgcgcagc ggcgcaatcg cttcgccctg ctctgcggcg cggcgtaaat ccgccgccag
    15961 ttgctcaaga gtatgcttcg tcatgaccat tccttaaggg cgtgcgagga aattcagcac
    16021 caattgattg aaagcgtcgg catgttccca ctgcgcccag tgaccacagt cgcggaagat
    16081 atgcagttca gaaccggcaa tgccggacag cagacgcaga cccgcatcca tcggcacaaa
    16141 gcggtcgttg cgcccccaga caatcagggt ttgcgctttg atttccgcca gacgtgggcc
    16201 aaaatccgga aactgtttcg gattagcttc caggctctta acgaagtttt ccaggtgatc
    16261 gcggcgcgac agcatattat tcaggcgcgc ttcaaacagg gcgtcggtca aatcgctggt
    16321 atcgaaaacg aagatatcca tcatcagctt caggttttcg atagtcggct gacgataaag
    16381 ctgattcagt cgcttaatac cttcggttgg catcggcgta aacaaactca tgccgcccgt
    16441 accgccgccc atcagcacca gtttgccgac gcgctccggc cagttaaggg tgaacgccac
    16501 agaactatgg ccgcccatcg agttgcccag caggtggatt ttggcgatat ccagttgatc
    16561 caccacgctt ttcaggattc gtgcattaag atccgatcgt gaaccactat taacgatcga
    16621 atcgcttttg ccccaacccg gacaatccag caggattacc cgatagcccg cctctaccag
    16681 cggatcgata ttgcggctga agttcgccca gccagtagca cccgggccgg aaccatgcag
    16741 caggacaacg gtttcgtcgc cttgtccgca gtcattaaaa tggatgcgca gcgttttacc
    16801 cgcttcttct acattcagaa aacggctggt ggcggcttcg gtttgtggct gataactcat
    16861 catcttctcc tgcatattca gttctctgtt ctggcgctta acgagccaaa tccggcaatc
    16921 cactccggga ttgggcggta ataacgccct tcgctacgcc agttgccaaa tgtagaaata
    16981 gcggcaaaag cggcgaccca ggttttgatt tcatgtgtcg actttccggc aatggcggaa
    17041 agctcttcgt tactgacggc atccagttcc tgtatgcgtc cctgctccag caaagtcatg
    17101 aactggttat cccaaatcgg gttgagcgga tgcagagttc tctgatcctc aacaaacttc
    17161 tcagcggcgc taatcacccg ttgctgacgc aattcgcgct cactggcggg taaatctttc
    17221 ccgctcccca acagacggtc gcgcatatgg gcatcggctt tcgccagttc gggcaccggc
    17281 ggctgatggg aaagcccacc ggaacccagg aacagcacgc gtttattgag agtgctggtg
    17341 aaacgtccaa tggcttcacc caacatgcgg gtacgctgga aaccgggcag cggcgtggcg
    17401 acaccgttga tgaacacagg cagaactggc accttatcca gcccaccgag caggaactcc
    17461 agcggctggg cgaacccgtg gtccacctgc atacagtaag aaacggcaag atcgatcccg
    17521 ctcttcatga cagcatgcgc acaggcctcc gccagctcca caggcacggg cagctctcct
    17581 gccgcactgc cgaaatcacc aattgccgtc gctccaacgc ctaaacagaa cggtggcatc
    17641 acgtcataga aaaagccgtt gtagtgatct ggcgcaaaca gcaccaccag ttcaggggag
    17701 aaagccgcaa tacgctcgcg ggcgctggca atcacgccat tgacctcatc gagtacctct
    17761 tgcgccgggt cgacataccc caccaacggc gagtgggaaa gacagtgaag ataagcgtgc
    17821 atatcaggct accttttcga cagaaacgtc ggcatcaggg cgggtcagcg tcatcaccga
    17881 cgccagttta ttcagcgtat tgcccagggt ttgcggaatg gcggtggcgg caacaaagcg
    17941 atccgggcgc atcaccacca gcgaagcatt gtgttgcgcg aaccagctac gcagacgacc
    18001 ttgcgtatcg cccacgcgta gcacgccgtc gtggttatcc tgtgcggtat gaatttgcac
    18061 ttccggcacc acctgaatga agcgtgtgcc caacgcgcgc cactgctgga tttgctcgtc
    18121 gctcatcccc cacagtggat tgcatcccca gccaattacc gcgaagttcg cgccaatcgc
    18181 gttatcgagc agcgtcacgt cgccgttttc cagcgtgact ttcggctgaa taaacatctt
    18241 gccgaccgga gagtgcttcg cttcgccctc acgtaccagc gcaccgccgt aatattgcgg
    18301 catcggcttg aagcgcattt cgaggaagta gcgttttact ggcggcagat aattcagtag
    18361 ccaggaaacg ccgtcacgta acgtaccctg ccagcgtttc ggcggagcca gcacgttgcc
    18421 cgccgtcacg gacaggtcaa tcatcgcttt ggcgtgatcg cgacgttctt gttgataggt
    18481 atcgagcagc gcatcgcggg ctttcccctg gataaccaac gccagtttcc aggcgaggtt
    18541 aaaggcgtcg cgcataccac tgttatagcc ctgcccctgc cacaccggca tgatgtgcgc
    18601 ggcatcgccc gccagcagta cgcgatcaat acggaaacgt tgcgccagtc gcgcgttatg
    18661 ggtgtagaca cgctggcgaa tcaattcaac attgtccgga ttaggcagca ctttgcttaa
    18721 cagcttgcgc atattttgcg gctcacgcag ctgctcttcg gtttctcccg gcatcaccat
    18781 aaattcaaag cgacgtaccg catgaggcag cgcggcagaa acatacgggc gcaccggatc
    18841 gcaacacaaa tagatatgcg gcgtacttaa cggatcgttg gcgatatcta ccacaatcca
    18901 ctgatttggc gcagttttac cttcaaacgg cacattcaga gtgcgacgga caaaacttgc
    18961 tccaccgtca caggctacca gccactgggc tttgactatt tcccgctgcc cttctgccgt
    19021 tttcaggtgc aaggtcactt cgtcattttg ctggctgaag gcctccagct cgcgggaaaa
    19081 caagcagcgc acattaggaa aacgcgacac cccttccagc atcaccgcat cgacctgcgg
    19141 ctgaataaag gcgttacggc gcggccagcc aaattcatcg gtcattggct gaatatcagc
    19201 aaaacagcgg cctttcgggg tgagaaaacg catcgcgtgc cacggcgtag tgtgcggcag
    19261 aacattctcg accaggccaa ccgactgcat ggtgcgcagc gcctcgtcat caataccaat
    19321 cgcacgcggg tagtcgatca acttatcgag tttctccacc accagcacgt caatgcccat
    19381 ctgaccgaga tagttcgcca tcatcagccc gaccggaccg gcacctgcga tcgccacctg
    19441 aacgctatgg ttaacagcag gctggatgtc agggtgttgt attgccattt cagtacctca
    19501 cgactcggac aaaatgccgt tgcgcgcaca gtacggcgca acttattttg ttaaaaacat
    19561 gtaaatgatt ttttgttgtg cgctcagtat aggaagggtg ttttcggcta caatcaaaac
    19621 atgcccgaat gtgcaccagg tgcacaacgt tgttttaact atagaaatgt caattaatat
    19681 gcagaacaat gagcagacgg aatacaaaac cgtgcgcggc ttaacccgcg gtctaatgtt
    19741 attaaatatg ttaaataaac ttgatggcgg tgccagcgtc gggctgctgg cggaactcag
    19801 cggcctgcat cgcaccactg tgcggcgact gctggagacg ctgcaggaag agggatatgt
    19861 ccgccgtagc ccctccgatg atagttttcg actgaccatc aaagtgcggc aattaagcga
    19921 aggatttcgt gacgaacagt ggatttctgc actggcggcc ccactgctgg gcgatctgtt
    19981 gcgcgaagtg gtatggccga cagatgtgtc cacgctggat gttgatgcaa tggtggtacg
    20041 cgaaaccact caccgtttca gccgcttatc ctttcaccgg gcaatggtcg ggcgacgttt
    20101 gccgcttctg aaaaccgcct cgggcctgac ctggctggcc ttttgcccgg aacaagaccg
    20161 caaggaatta atcgaaatgt tagcctcccg ccccggtgat gactatcaac tggcacggga
    20221 accgttaaag ctggaagcca ttctggcgcg cgcgcgcaaa gagggttacg gacagaacta
    20281 ccgcggctgg gatcaggagg agaagatcgc ctctatcgcc gtaccgctgc gcagtgaaca
    20341 acgggtgatt ggctgtctga atctggtgta tatggcgagc gcaatgacca ttgaacaggc
    20401 agcggaaaag catcttccgg cgctacaacg ggtagcaaaa cagatcgaag aaggggttga
    20461 atcgcaggct attctggtgg ccggacggcg aagcggcgtg catttacgtt gacaccatcg
    20521 aatggcgcaa aacctttcgc ggtatggcat gatagcgttc ggaagagagt caattcaggg
    20581 tggtgaatgt gaaaccagta acgctatacg atgtcgcaga gtatgccggt gtctcttatc
    20641 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
    20701 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
    20761 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
    20821 aaattgtcgc ggcgattaaa tctcgcgctg atcaactggg tgccagcgtg gtggtgtcga
    20881 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
    20941 gcgtcagtgg gctgctcatt aactatccgc tggatgacca ggatgccact gctgtggaag
    21001 cagcctgcgc taatgttccg gcgttatttc ttgatgtctc tgaccagact cccatcaaca
    21061 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
    21121 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
    21181 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
    21241 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
    21301 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
    21361 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
    21421 acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc
    21481 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
    21541 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
    21601 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    21661 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    21721 ttacacttta tgcttccggc tcgtatgttg tgtgaaattg tgagcggata acaatttcac
    21781 acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga
    21841 ctgggaaaac cctggcgtta cacaacttaa tcgccttgcg gcacatcccc ctttcgccag
    21901 ctggcgtaat agcgaagaag cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
    21961 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
    22021 gtgcgatctt cctgaggccg atactgtcgt catcccctca aactggcaga tgcacggtta
    22081 cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc cgtttgttcc
    22141 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
    22201 ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc tgtggtgcaa
    22261 cggtcgctgg gtcggttacg gtcaggacag tcgtttgccg tctgaatttg acctgagcgc
    22321 atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag
    22381 ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct
    22441 gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta atgatgattt
    22501 cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg actacctacg
    22561 ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg
    22621 cggtgaaatt atcgatgagc gtggtagtta tgccgatcgc gtcacactac gtctgaacgt
    22681 cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact
    22741 gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt tccgcgaggt
    22801 gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gaggcgttaa
    22861 ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga
    22921 tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca
    22981 tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg aagccaatat
    23041 tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc
    23101 gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat
    23161 ctggtcgctg gggaatgagt caggccacgg cgctaatcac gacgcgctgt atcgctggat
    23221 caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg acaccttcgc
    23281 aaccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccaaccct tcccggcggt
    23341 gccgaaatgg tccatcaaaa aatggctttc gctgcctgga gaactgcgcc cactgatcct
    23401 ttgcgaatat gcccacgcaa tgggtaacag tcttggcggt ttcgctaaat actggcaggc
    23461 gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct
    23521 gattaaatat gatgaaaacg gcaacccgtg gttggcttac ggcggtgatt ttggcgatac
    23581 gccgaacgat cgccagttct gcatgaacgg tctggtcttt gccgaccgca cgccgcatcc
    23641 ggcgctgacg gaagcaaaat accagcagca gtttttccag ttccgtttat ccgggcaaac
    23701 catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat
    23761 ggtggcgctg gatggcaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca
    23821 aggtaaacag ttgattgaac tgcctggact accgcagccg gagagcgccg gacaactctg
    23881 gcttacggta cacgtagtgc aaccgaacgc gaccgcatgg tcagaagccg gacacatcag
    23941 cgcctggcag cagtggcgtc tggcggaaaa cctcagcgtg acactccccg ccgcgtccca
    24001 cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg gtaataagcg
    24061 ttggcaattt aaccgtcagt caggccttct ttcacagatg tggattggcg atgaaaaaca
    24121 actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg acattggcgt
    24181 aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg cggcgggcca
    24241 ttaccaggcc gaagcagcgt tgttgcagtg ctcggcagat acacttgccg acgcggtgct
    24301 gattacgacc gctcacgcgt ggcagcatca gggaaaaacc ttatttatca gccggaaaac
    24361 ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tagcgagcga
    24421 tacaccgcat ccggcacgga ttggcctgac ctgccagctg gcgcaggtcg cagagcgggt
    24481 aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg cggcctgttt
    24541 tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa
    24601 cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc gcggcgactt
    24661 ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc atcgccatct
    24721 gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg
    24781 cgacgactcc tggagcccgt cagtgtcggc ggaattccag ctgagcgccg gtcgctacca
    24841 ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc tgcccgtatt
    24901 tcgcgtaagg aaatccatta tgtactattt aaaaaacaca aacttttgga tgttcggttt
    24961 attctttttc ttttactttt ttatcatggg agcctacttc ccgtttttcc cgatttggct
    25021 acatgacatc aaccatatca gcaaaagtga tacgggtatt atttttgccg ctatttctct
    25081 gttctcgcta ttattccaac cgctgtttgg tctgctttct gacaaactcg ggctgcgcaa
    25141 atacctgctg tggattatta ccggcatgtt agtgatgttt gcgccgttct ttatttttat
    25201 cttcgggcca ctgttacaat acaacatttt agtaggatcg attgttggtg gtatttatct
    25261 aggcttttgt tttaacgccg gtgcgccagc agtagaggca tttattgaga aagtcagccg
    25321 tcgcagtaat ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct gggcgttgtg
    25381 tgcctcgatt gtcggcatca tgttcaccat caataatcag tttgttttct ggctgggttc
    25441 tggctgtgca ctcatcctcg ccgttttact ctttttcgcc aaaacggatg cgccctcttc
    25501 cgccacggtt gccaatgcgg taggtgccaa ccattcggca tttagcctta agctggcact
    25561 ggaactgttc agacagccaa aactgtggtt tttgtcactg tatgttattg gcgtttcctg
    25621 cacctacgat gtttttgacc aacagtttgc taatttcttt acttcgttct ttgctaccgg
    25681 tgaacagggt acgcgggtat ttggctacgt aacgacaatg ggcgaattac ttaacgcctc
    25741 gattatgttc tttgcgccac tgatcattaa tcgcatcggt gggaaaaacg ccctgctgct
    25801 ggctggcact attatgtctg tacgtattat tggctcatcg ttcgccacct cagcgctgga
    25861 agtggttatt ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg tgggctgctt
    25921 taaatatatt accagccagt ttgaagtgcg tttttcagcg acgatttatc tggtctgttt
    25981 ctgcttcttt aagcaactgg cgatgatttt tatgtctgta ctggcgggca atatgtatga
    26041 aagcatcggt ttccagggcg cttatctggt gctgggtctg gtggcgctgg gcttcacctt
    26101 aatttccgtg ttcacgctta gcggccccgg cccgctttcc ctgctgcgtc gtcaggtgaa
    26161 tgaagtcgct taagcaatta atgtcggatg cgccactggg cacatccgac aattatttat
    26221 agctccatca tctggcgaaa caagcgtcgc atcatacctc tttgacccac agctgcggaa
    26281 aacgtgctgg tgcaaaacgc agggttatga tcatcagccc aacgacgcac agcgcatgaa
    26341 acgcccagtc catcaggtaa ttgccgctga tactacgcag cacgccagaa aaccacgggg
    26401 caagcccggc gatgataaaa ccgatcccct gcataaacgc caccagcttg ccagcaatag
    26461 ccggttgcac agagtgatcg agcgccagca gcaaacagag cggaaacgcg ccgcccagac
    26521 ctaacccaca caccatcgcc cacgataccg gcaattgcag cggcaaccag ataaagccgc
    26581 agaaccccac cagttgtaac accagcgcca gcattaacag tttgcgccga tcctgatggc
    26641 gagccatagc aggcatcagc aaagctcctg cggcttgccc aagcgtcatc aatgccagta
    26701 aggaaccgct gtactgcgcg ctggcaccaa tctcaatata gaaagcgggt aaccaggcaa
    26761 tcaggctggc gtaaccgccg ttaatcagac cgaagtaaac acccagcgtc cacgcgcggg
    26821 gagtgaatac cacgcgaacc ggagtggttg ttgtcttgtg ggaagaggcg acctcgcggg
    26881 cgctttgcca ccaccaggca aagagcgcaa caacggcagg cagcgcccac caagcgagtg
    26941 tttgatacca ggtttcgcta tgttgaacta accagggcgt tatggcggca ccaagtccac
    27001 cgccgcccat cagagccgca gaccacagcc ccatcaccag tggcgtgcgt tgctgaaacc
    27061 gccgtttaat cactgaaggc atcaccgcct gaatgatacc gatccccacc ccaccaagca
    27121 gtgcgctgct aagcagcagc acactttggg ggtaaagctc acgcatcaat gcaccgacgg
    27181 caatcagcaa cagactgatg gcgacactgc gacgttcgct gacatgctga tgaagccagc
    27241 ttccggccag cgccagcccg cccatggtga ccaccggaag agcggtcaac agggcagcca
    27301 cgctaaagct cattccgctc gcctggcgca attgcggtag cagtggcccg acggaggtga
    27361 gcagtggtcg catattaaga ccaatcagca ccagaaccag cagcatcgtg aacgtctttt
    27421 gatgattgcg catggctgtc agaacggctt ggtcggcaga tatttaccat ctaaggtaat
    27481 gaccgcacgt tcgccacctt ccgggtccgc cactttctta acgtcgagtt tgaagttaat
    27541 cgcgctgata atgccatcgc caaacttctc atgaaccaac gctttcaggg ttgtgccgta
    27601 cacctgcaac atttcataga aacgatacat cgttgggtcg gttggaatac ggtcatcaat
    27661 gcagccacgc agtggaatca tctgcaacag tagaatggcg tcttcgtcga gatccagctt
    27721 cgccccgacc aggcgggcgg cgtcggcagg aagcgcttgc tgccccagca aagccgcggt
    27781 tacgaaggct tctgccagac cggtaccgtc ggcaatctcg gcaaatgaga gatctttttt
    27841 agctttgctg agcaaaatgg catcggcaag atcaagacga atattgcggt taatttgtga
    27901 ctgaatcatg gtggatctcc tgatggttta aaaataaggt tacgctgcgg tcggttggcg
    27961 tagcggtatg gcacaaacgc gaggattagc ggccagtggc acaaactggc gggttgcgcc
    28021 gtcaaaagct gcgatgctgc cgctttcaat gtcgtagacc cagccgtgca gggcgatccg
    28081 cccctcttcg agcgccaggc gcaccgatgg atgagtttgc aaattagcca actgagcaat
    28141 gacgttttca cgtaccatcg ccgcagcttt tgacggtaaa tcggaatgcg ggcgcgcctc
    28201 attaacgacg cgggctgaat cggcataacg cagccagtgg gagacggcag gcatatggtc
    28261 catgcactga cagctggcaa tggcggtcat cgcgccacag ttggaatgac cacaaatcac
    28321 aatgtcagat acccgaagcg cagcgacggc atactccacc gaagcagaaa cgccaccggg
    28381 ttccggcccg taggaaggga cgatattgcc cgcgttgcga ataacgaaca gatcgccagg
    28441 ctcacgttgc gtcaccagct cagggaccag acggctgtcg gagcaggaga taaaaagtgt
    28501 gcgcgggctt tgctgtgtcg ccagctgttt aaacaaggct tcccgcttcg gaaatgcctc
    28561 gcgctggaat ttaaggaatc catcaataat ctctttcacc gttaacctct gtctgtctct
    28621 ggaatgagag gcagactact cgccgattat cataaggtaa aagtctcatt tatgatgagt
    28681 tctattggat ttacttatag gttggcgaat gctctctcga catatcaatt attttcttgc
    28741 cgtggctgaa catggcagct tcacccgtgc cgccagtgcg ttgcacgtct ctcagcctgc
    28801 gctttcccag cagattcgcc agttagagga gagtttaggc gtgccgctgt ttgaccgtag
    28861 cgggcgaacg attcgtctca ccgatgcagg agaagtctgg cgacagtacg ccagccgggc
    28921 gttacaggaa ctgggagcgg gtaaacgggc gattcatgat gttgccgatc tgacgcgagg
    28981 atcgctgcgt atcgccgtca cccccacctt tacaagctac tttatcggcc ctttaatggc
    29041 ggatttctat gcgcgctacc ccggcatcac gctccagcta caggaaatgt cgcaggagaa
    29101 aatcgaagat ctgctttgtc gcgacgagct ggacgttggg attgccttcg cgcctgtgca
    29161 ttcgccggag ctggaggcaa ttcctttact gacagaaagt ttagcgttag tcgtggcgca
    29221 acatcatccg ctggctgcct gtgaacaggt ggcgttgagt cgcttgcatg atgaaaaact
    29281 ggtcctgctc agcgcggaat ttgccaccag agagcaaatt gaccactact gcgagaaagc
    29341 ggggctacat ccacaggtgg tcattgaggc gaactcaatt agcgcggttc tggagctgat
    29401 tcgccgcact tccctttcca cattgttacc agcagcgatt gccacacaac atgacgggct
    29461 taaagcgatt tctcttgccc cgccactact ggagagaacg gcggttttgt tgcggcggaa
    29521 aaatagctgg cagacagccg ccgcgaaggc atttttgcac atggcgttgg aggaatgtgc
    29581 ggatgttggc gagaatgaat cacggtagcc actgacacag gtcagtggct cttcaacaca
    29641 gaggaaatta ccgtttgtaa tcgatggctt ctggctgctc cagatatacg gtggtttgtg
    29701 ccggttgtgt actggcaatc accttgccgc cacgtaccga ataacgtacc ggaacctgac
    29761 ggcgcagcgc atcaaatcca ttttcagccg gcaggataat caggtttgcg ctgtttccgg
    29821 cggcaatgcc gtaatcctgc aaattcaatg ttctggcact gtggtgggtg attaaattca
    29881 ggccatcgtt aatctgcccg tagcccatca actggcaaac atgcagcccc atatgcagca
    29941 cttgcagcat attcgccgtt cccagcggat accacggatc gaagacatca tcgtgaccaa
    30001 agcagacgtt aatgccggac tccagcatct ctttaacgcg cgtgatgccg cgacgttttg
    30061 gatacgtatc gaaacgtcct tgcagatgaa tattgaccag cgggttggcg acaaagttaa
    30121 taccggacat tttcagcaag cggaacaggc gtgaggtata cgccccgtta taggagtgca
    30181 ttgccgtggt gtggctggcg gtgactcgcg cgcccatgcc ttcacggtgc gccagggcag
    30241 caacggtttc gacaaagcgc gactgctcgt catcgatctc atcacagtga acgtcgatga
    30301 gacggtcgta tttttgcgcc agggcgaagg ttttatgcag cgactccacg ccgtattcac
    30361 gggtaaattc aaaatgcgga atcgccccca ctacatctgc ccctaagcgt aacgcctctt
    30421 ccagcaacgc ttcaccgttg ggatacgaca aaatcccttc ctgagggaag gcgacaattt
    30481 gcagatcaat ccacggcgcg acttcctgct tcacttccag cattgctttc agcgcagtta
    30541 gcgttgcatc cgaaacatcg acatgggtac gcacatgctg aatgccgttg gcaatctgcc
    30601 atttcagcgt ttgccaggcg cgttgtttca catcgtcatg ggttaataac gctttgcgct
    30661 cggcccagcg ttcaatgcct tcaaacagcg tgccggactg attccagttc ggttgtccgg
    30721 cggtttgcgt ggtgtccagg tgaatatgcg gctccacaaa cggcggtata actaaacctt
    30781 gttcggcatc caggctgttt tcagttatgg gcatcacgcc ggattgcgca tcaatggcgc
    30841 tgatttttcc gtcctgcaga tgaatctgcc acagcccctc tttgcctggt aaccgggcgt
    30901 taataattgt ttgtaaagcg ttattcgaca ctgttaacct ccacatgcgt cattgctgct
    30961 gtcgttttac gattcaaaat cgggttaagg atcagatagc tcagcgcgcc acctaatacc
    31021 gcgttgaccg gaacaattcc cggtaaccag tggcctgcgg caatccccag cgcgaccgcc
    31081 agaatcgcca cccaattgac actcatcata cgcgtggtcg caaagtgctc atagcggcga
    31141 cggttcatca gatagtcggc gatgatcacg ccccctactg gaggaatagc tgccgaaagg
    31201 aaggtcaacc agccgacaaa attgttatac agccataatg cgcagaccgt accgataata
    31261 ccgttgatta ccgaaagggt tttgctcgac ataccggtaa tgttggcgaa acctaaaccc
    31321 gacgcataga gtgcgttatc gttggtggtc cagatattca gccccagcac cacaatcgca
    31381 ggcagcagca ggccctgagc aatcatcaca tcagagatat ccgccatgcc cagtgccgca
    31441 gcgcccgctg caccgaaaat aaacatcaac gagttgccga ggaaaaaggc caccatcgcc
    31501 accagcaccg ccagtttggc attgcgacca aaccggacaa agtcagcggt gagcgtaccc
    31561 gcactgataa atgaccccac aaccagcgcc agcgcgacat tgaaatctaa cggttgtgcg
    31621 ggaacgaccg cttttaatgc gtccaggccg cccatgccgt taacagccag ccacacggaa
    31681 taaccgccca ggcaggcgat agccggaacc gcaatcaccg aaagaaccgt cagcgccgaa
    31741 atgccaaaaa agacggtgac ggtcatcagt aaaccggaaa cggcaatcag caaattaata
    31801 tccagcccgg ttgccttacc caccggaatg gcaaacatcg ccacaccgac gccaaaccag
    31861 ccaacctgag ttccgcccag tagcagtgaa ggcagccatg agcctttaac accaaacgag
    31921 aagcgagcaa gaagatgagt ggtcaggccg gtttttgcgc caatgtaacc gagaaatgaa
    31981 gtgtaaatac cgaggagaag attaccgatg agaactgcga ggaagaaatc atgatagcta
    32041 agaccggttc cgagagtgcc gccggtccac atactggcgg aaaagaaggt taatcccagc
    32101 atgacgaacg tcaatgccaa tacccctttc cgcgccgact gcgggactgg cccctggcta
    32161 aagttgttat cttgcgacac gaaattcccc ctttttgctg tttgaaaccc aaaaaatccg
    32221 ccgcattcta ttcatctgaa gataaaaagc aatcgttttc gtggggaaat atatttttta
    32281 tatgaatgag tgcaaaaagg tgggggaaaa attatcggtg taaaaacagt gggtagtggg
    32341 tagatactga taagacgcgt cagcgtcgca tcaggcatcg gcgcggggtg tcggatgcga
    32401 cgcgagcgcc ttatccgacc tacggttcag gcctgtaggc ataataagac gcggtaagcg
    32461 tcgcatcagg catcggcgca tggtgtcgga tgcgccacgc gacgcatccg acaataccct
    32521 actcttccat cgcctggcgg atctgatcca atgacgcagg atcatcaatg gtcgtcagat
    32581 ccccaggatc gcgtccttcg caaattgcct ggatcgtgcg gcgcagcatt tttccggatc
    32641 gcgttttcgg caattgcgag acaaaccaga cgtgcgccgg gcgaccaaag ttgccaatct
    32701 ggctgtccac cagcgccata atcgccttct cttgcgaatg cgccacatca cgatcttcca
    32761 gactatcgct ctctttcgga atgacaaacg ccaccgccac ctgccctttc agcgcatctt
    32821 tcaccccaac caccgccact tcggcaacgc ccggatggct ggagatactc tcttcaatct
    32881 cgcgcgtgcc cagacgatgc ccggcaacgt taattacatc gtcagtgcgc ccgagaataa
    32941 agtgataacc gtcagcgtca cggatgcccc agtcaaaggt ggcgtacacc gggcgggaaa
    33001 acagcgacca gtaagtcttc acaaagcggt cgtcgtcgcc ccagatggtc tgaatacagc
    33061 ccggcggcag cggcccttcc accaccagca tccctttctc gttgacgcca cacggttcgc
    33121 cggtgacttc attaagcaac tgcacgttat agccatacat cggcacgccg gggcttccca
    33181 gacgcgtcgg cctgtcgtcc agaccgcgag caatcgccat aatcggccag ccggattcgg
    33241 tctgccagta gttgtcgatg accggcacat ccagcgtatt gctcacccaa ctggcggtcg
    33301 gctcgtccag cggttctcca gccagataga gcacttccag cgacgagaga tcgtgtttgc
    33361 gaatttcagc ggtagggaat tttttcagca cgcgaatggc ggtcggcgct gagaacatcc
    33421 ggctaacctg atatttctcg acgattttcc accacacgcc gcagtccggc caggtcggca
    33481 atccttcgta aacgatagtc gccatccccg ccagcagcgg cgcgtaaacg atatacgaat
    33541 gccccaccac ccagccgata tccgatgcgc aaaagaacac gctgcccgct ttgccgccaa
    33601 aaatggtgtc catcgaggtc gccagcgcca ccgcatatcc gccgacgtca cgctgcacgc
    33661 ctttaggttt gccggtcgtg ccggaagtgt agagaatgca ggaggtttcg ttggattcca
    33721 gccacgccac cggtacccgc gcgccgatgt gttgatggcg caacgacgcg aaatcgacat
    33781 cccgcccgct gacgcgcgcc attttcgcca gcccgcgatc caccagcaaa acatggcgtg
    33841 gctggtgctg cgcctgactt atcgcatcgt cgagcaattt tttataggga atgattttgc
    33901 caccgcgcgc tccggcatca gccgagacaa tcagcaccgg tttagcgtca tcaattcgcg
    33961 ccgccacgct gtgcgaggca aaaccaccaa ataccaccga atgaatagca ccaatgcgcg
    34021 cgcaggccag cagagtaata tgcgcttcgg caatcatcgg catatacacc agcacccgat
    34081 cgccgcgctg cacgcccagt gagcgcaaca ttgaggccac cgcgttcact tcgtcatgca
    34141 gctggcgaaa ggtaaaggtg cgctcttctt ctgtttccga agagacggca atcaatgcca
    34201 gtgcctctgg ctgtttctcc agccagcggt cgatagcgtt gtggcacaaa ttggttcggc
    34261 cttcacaaaa ccaacgggca aacggcggat tgctgtggtc gagcgtttgc gtaaagggcg
    34321 tctgccagtc aatacgccgg gcctgctcgg cccagaactg ctccggttcg ttaatcgaac
    34381 gctgataaaa ttcgctaaaa gacataatgc tctcctgttg aacttacgcc ttaccgccgt
    34441 ttacttaaat gacgtacagg tcgagatact cattgaccgg catctgttcc aggcgagttc
    34501 tgtcgagaga aacctccaga atgcgctgct gttggcgagt cgggaactgg cgcgcgagat
    34561 tgattttgaa tttatcgacc agtttcggaa taccatcctg acggcggcga gcatgaccaa
    34621 tggggtactc caccaccact tcttcaaatc gtgtgccgtc ggtgaactca agggtaatgg
    34681 cattggcgat ggcgcgtttt tccgggtcgt ggtagtcagc ggtaaatgcc ggatcttcaa
    34741 agcaattgat cttctcgcgc agggcgtcga tgcgtttatc ttgcgcaacg ttgtcctcgt
    34801 aatctgccgc cgttaagcgc ccgaataaca gcgggatcgc caccatgtac tgaatgcagt
    34861 ggtcacggtc agccgggtta ttgagcggcc cctttttgtc gatgatgcga atacaggctt
    34921 cgtgggtgcg aatggtcact ttttcgatat ccgccgccgt cttgcctgct gcctgcatct
    34981 gttcatagag cgtcatcgcc gcttcaactg ccgtctggga gtggaactcc gccgggaagg
    35041 agattttgaa cagcacattc tccatgacgt aagaaccgta cggacgctgg aagcggaacg
    35101 attcaccttt aaaggagacg tcgtagaagc cccataccgg cgcggtcagg gctgacgggt
    35161 aacccatttc gcccgttttc gccatcagtg ccagacgtac cgcgcgggaa gtggcatcgc
    35221 ccgccgccca ggatttacgc gtgccggtgt tcggcgcatg gcgataggtg cgcagcgact
    35281 gaccgtccac ccacgccagc gaaacggcgt tgagaatttc ctcgcgggtc aggccgagca
    35341 tttcggcgac cacggcggtg gaagccactt tcactaacag aacgtggtcg aggccgacgc
    35401 ggttaaagga gttttccagc gcgatgcagc cctgaatttc atgggctttg atcattgcgg
    35461 tcagcacctg tttcatggtc aacggcgctt tgccgctggc gacagcgttg cgcgaaagcc
    35521 agtctgccgt tgccagaatg ccgccgaggt tgtcggaagg atgcccccac tccgccgcca
    35581 gccaggtatc gttgaaatcg agccagcgga tcatcgcgcc aatgttaaat gccgcctgga
    35641 cagggtcgag ctgaaactga gttccgggga cgcgcacgcc gttgggtacg acggtgccag
    35701 gaacaattgg ccccagcagt tttttacagg ccgggtattc gagagcttcc agaccgcagc
    35761 cgagcgtgtc gagcaggcag taatgtgcgg tgtcgtaggc cactttagag ctgatttcgt
    35821 agttcatcac gtaatcgacg atatcaacga tttcacgatc aaattccggg cggatgttgt
    35881 tgatttgagc tgacattggg tatgtttcct tattgttatt cgtagaggtt tactggcgct
    35941 tatccagcgc gacaaactgg cggtcttccg gtccaacata attggcggaa ggacggataa
    36001 ttttgttgtc ctgacgttgt tcaatgatgt gcgccgccca gccggtgaca cgggcgatga
    36061 caaacagtgg tgtgaacatc tcggtgggaa cgcccatcat gttgtaggaa acagcagaga
    36121 accagtcgag attggggaac atctttttgc tctcccacat caccgtttcc aggcgattgg
    36181 cgatgttgta catcttcaac gagccgcctt cctgcgagag ctgcttcgcc acacgtttga
    36241 tcacctggtg gcgcgggtcg gcgatggtat aaaccggatg accaaaacca atgaccactt
    36301 ctttgttttc cacccgcttg cggatatcgg cttcggcttc gtccggcgtt tcgtagcgtt
    36361 gctggatctc cagcgacact tcattcgctc cgccgtgttt cggcccgcgc agtgcgccaa
    36421 tcgcgccaat aatcgcggaa tacatatcag agccagtgcc cgcaatcacc cgactggtaa
    36481 aggtggaggc gttaaactcg tgttcggcgt acagcactag cgaaatatgc atcgcctttt
    36541 cccagctttg cgatggcttt tcgccgtgca gcagatgcag gaagtgacca ccgatggagt
    36601 cgtcatcggt ttccggctgg atgcgttcgc cgttgtgact gtagtgatac caataaagga
    36661 gaatcgagct aagtgatgcc agcagtttgt cggcaatatc ccgcgcgcca gagacagtgt
    36721 gcccctcttt ttctggcagt gtgcagccga gcgcggaaac gccggtgcgc atgacatcca
    36781 tcgggtgcga cgccgccggt aaggcttcca gcacggtacg cacgttagcc ggtaaaccgc
    36841 gcagggcttt cagtttcgtt ttgtaagcgg cgagttcgtc acgggtcggc agtttgccgt
    36901 ggatcagcag gtgcgccact tcttcaaatt cgcaatgttc cgccagatca agaatatcgt
    36961 agccacggta atgcaggtca ttgccacttt tacccacggt gcagagcgcc gtattgcccg
    37021 ccggaacgcc agaaagtgcc acagattttt tcggtttaat gacatgggta ctgttttgca
    37081 ggatcgttgt gtcgctcatg ttgtcctcgt catttgtagg gtcgagatat ttttcgttgt
    37141 gttttatttc acctggctac gggcaaacag gtcgtcgagc ttcgcttcgt actggtagta
    37201 gttgatgctt tcgtacagct cgttgcgggt ttgcatggta tcgatgacgc ttttctgcgt
    37261 gccttcctga cgcaggacgt tgtagacatg ctcagcggcg cggttcatgg cgcggaacgc
    37321 tgaaagcggg tacagcgcca ttgcgacatg ggcgctgcgt aattcgtcga tggtaaacag
    37381 cggcgtggca ccaaattcgg taatgttggc gaggattggc acctgcaccg catcggcaaa
    37441 ctggcggtac atggcgagtt cggtaatcgc ctccgggaac aacatctcgg cacccgcttc
    37501 aacataggcc tgcgcacgct cgatcgccgc atccagcccc tctaccgcca gagcatcggt
    37561 gcgcgccatg atcacaaaat caggatcggt tttcgcatcc accgccgcgc ggatccgatc
    37621 caccatctct tctttcgaga cgatcgcttt attcggacga tgaccgcagc gtttcgcacc
    37681 aacctgatct tcaatatgca atcctgccgc accggcttta atcatcgatt tcacggtgcg
    37741 cgccacgtta aaggccgaag aaccaaaacc gatatccgca tccaccagca gcggcagcga
    37801 acaaacgtcg gtgatacggc gaatatcggt cagcacgtca tcaagagtag aaataccgag
    37861 atcgggcagc cccagcgaac ctgccgccac gccaccgcca gaaagataaa ttgcctgata
    37921 tccggcacgc tgcgccaaca gcgcatgatt agcgttgatg gtgccaacaa tctgcaatgg
    37981 attttcttta gtcagtgcag cgcgaaacgc tttacctgga gagtgtagag acatagccca
    38041 tcctttgtta tcaacttgtt atttgcgttg ataaagacaa agcaaggggt gtgccacaat
    38101 tgccttaata aaattaaatg tttaatttca atttgttaga gataaagtca tgaactaatc
    38161 cgcaaatatg cgtttcagtt aacgtttcag gcaatgtttc accgcgtttc attgcaacaa
    38221 ttatgaaaca agactaaacc caatattcgg tttcttaact ttgcggtgcg ctatggcaca
    38281 tccaccacgg cttaatgacg acaaaccggt tatctggacg gtatctgtaa cgcgcctgtt
    38341 cgagctgttt cgcgatatca gcctcgagtt tgatcacctg gcgaacatta cccctatcca
    38401 gcttggcttt gaaaaagcgg tgacctacat ccacaagaaa ctggcaaacg aacgctgtga
    38461 cgccatcatc gcggctggat ctaacggcgc gtacctgaaa agccgcctgt cagtgccagt
    38521 tattttgatt aaaccgagcg gctacgatgt gttacaggca ctggcaaaag ccggaaaact
    38581 cacctcttct atcggcgttg tcacttatca ggaaactatt ccggcactgg tggcgtttca
    38641 aaaaaccttt aatttgcgcc tcgatcaacg tagctacatt accgaagaag acgcacgcgg
    38701 gcagattaac gagctaaaag ctaacggcac cgaagcggtg gtcggcgcgg ggctgattac
    38761 cgatctggca gaagaagccg gaatgaccgg aatttttatc tattccgccg ccaccgtgcg
    38821 ccaggcgttc agcgatgcgc tggatatgac gcgcatgtcg ttacgccata acactcacga
    38881 tgccactcgc aacgccctgc gtactcgtta cgtgctgggc gatatgctcg gtcaatcacc
    38941 acagatggaa caggtgcggc agactatttt gctgtatgcc cgctccagtg cggcggtgtt
    39001 gattgagggg gaaacgggga cgggcaaaga actggcagcc caggcaattc atcgggaata
    39061 ctttgcccgc cacgatgcgc gacagggcaa aaagtcgcat ccgtttgttg ccgtcaactg
    39121 cggggcgatt gccgaatcgc tgctggaagc ggagctgttt ggctatgagg aaggggcgtt
    39181 taccggctcg cgacgcggag gtcgcgccgg gctgtttgaa atcgcccacg gtggcacgct
    39241 gtttttggat gagattggcg aaatgccgct acctttgcag acccggctgc tgcgggtgct
    39301 ggaagaaaaa gaggttaccc gcgtcggcgg gcatcagcct gttccggtgg atgtgcgggt
    39361 cattagcgcc actcactgca agctggaaga agatatgcgg caagggcagt ttcgccgtga
    39421 cctgttttat cggctgagca ttttacgcct gcaactgcca ccactgcgtg agcgggtggc
    39481 ggatattctg ccgctggcgg aaagcttttt gaaagtgtct ctggcggcgc tctccgcccc
    39541 gttttctgcc gcattacgcc tgggattaca ggcaagcgaa accgtgctgg tgcactacga
    39601 ctggccgggc aatattcgtg aactgcgcaa tatgatggag cgactggcgc tatttttaag
    39661 tgtggaaccg acgccggatt taacgccgca atttttgcag ctgctactgc cggaactggc
    39721 gcgcgagtcg gcgaaaactc ccgctccacg cttactgaca ccacaacagg cactggagaa
    39781 atttaatggc gataaaacag cagcggcgaa ttatttaggc atcagccgga cgacgttctg
    39841 gcggcggctg aaaagctgaa ctgccgaatg cagcgcgaac gctttatccg gcataaattt
    39901 caatagatga tcaccgataa acttacgcat cgggcatgtt tacatagggt tcagaattac
    39961 ttcttcttat aaatatttgc cgtgccgtgg atcttattgt cagtttgacc ggaggtcagt
    40021 accaacacat cagccccttt ttcatccgct tttttgatca aatcttcttt cgcgtctgca
    40081 gtcgacattt cattgctggt tgaaatatca cctatttttt catactgcga ttcaactttt
    40141 tcaaattccg ctttggtcat caattcagcg gcatagacat tggtaacggc taacgctaac
    40201 gcaccgacta acattttaga gattattttc atattacggc tccagagttg ttatcacact
    40261 atcatccacc cacattattg ggttgattaa taattagttc agtcagggca taaacgccag
    40321 taaccggaaa taagacactt tgtgatctct ctcgcacccg attatttttg gtagaaatat
    40381 gatgaagtta cttcacttat ttatggatga aatcactatg gatcctttgc acgccgttta
    40441 cctgaccgta ggactgttcg tgattacttt ttttaatccg ggagccaatc tctttgtggt
    40501 agtacaaacc agcctggctt ccggtcgacg cgcaggggtg ctgaccgggc tgggcgtggc
    40561 gctgggcgat gcattttatt ccgggttggg tttgtttggt cttgcaacgc taattacgca
    40621 gtgtgaggag attttttcgc ttatcagaat cgtcggcggc gcttatctct tatggtttgc
    40681 gtggtgcagc atgcgccgcc agtcaacacc gcaaatgagc acactacaac aaccgattag
    40741 cgccccctgg tatgtctttt ttcgccgcgg attaattacc gatctctcta acccgcaaac
    40801 cgttttattt tttatcagta ttttctcagt aacattaaat gccgaaacac caacatgggc
    40861 acgtttaatg gcctgggcgg ggattgtgct cgcatcaatt atctggcgag tttttcttag
    40921 tcaggcgttt tctttgcccg ctgtgcgtcg tgcttatggg cgtatgcaac gcgttgccag
    40981 tcgggttatt ggtgcaatta ttggtgtatt cgcgctacgc ctgatttacg aaggggtgac
    41041 gcagcggtga gcagataatt tactctctac gtaatcaacc tgatttgccg aatcccgacc
    41101 atcggcgatg attgcccccg taatgcgtgg tccttatttc cgataagttt acttgagtct
    41161 ttagcttccc atttcagccc actctcccct gtgtgggctt ttttcattgg tctgacccgt
    41221 cctgaatagc caaacaatgg caatgttcca ctttgcaggg atgataataa ggggatctga
    41281 ttcagaagag ttttgaaaag ttggaccgcc atcgaggcct cgaaccccgt accatacaac
    41341 aatcctggtt gtcgtctgct cttcctgatg agctaatggc ggtttttgat atgagcctta
    41401 cagcatattt tctgtcaggc ttgagtgcga tgctaaggta cagaaaagcc cccacacaaa
    41461 catgatggaa ttatgatttt tttcatatga atgtcaaccc acacattcac aaaacatctg
    41521 catgagatca ttttttatac accgcatagc tgcgaaacca ctgctggtgc ttttttaagc
    41581 gaagcgtcgc cgatcacaac aaaatatcgg cgaaaaaggc ggtggaatgc catttggcat
    41641 acggatatac agatgaaatg attacctgct ttgcgattca ctctctggtg gaataacaac
    41701 ttgcaggtgg aaccctttct gaactaaaga gtgtagcgga ttttaacgtg cttatttaca
    41761 ggataatatc tgtcacctaa ttcctgcact ctattcgata tccattcatt taatcgtgcg
    41821 tcatctctgt atgtttttac gtccagaaca gagagcaata acatcaccag tgattcagga
    41881 aaaacagggt ggatattttt taagtgtaat aataatccat gcgcaagcgc ctgcttaatg
    41941 ggcgcgtctg tctccggaat agacgacatt tcgcgaagcc atttttccac acgcatacac
    42001 tctttccctt tagggaaaat tctcagcaaa ggcatatggt gttcaagaag aatacatttg
    42061 caatctgttc gcactgaatc gataaactgc actaatttct taagcccggt aaagcgtcca
    42121 gcctgcacat ttgctttaat atcaagaaac gcatcatcct ggtcatatac ccaaactgtc
    42181 cctttttgat tttcaagtat gcgaaaaaca aacttaaccg ggtgatcagg gtaatccatc
    42241 cagtcataaa atccagggaa atcattaaac tgcacattta tggtgagtgc gttatcgtct
    42301 gaccactcac agtctcgtac ggcagccatg agatgaatga gtttgtcgtc atcgactaaa
    42361 tcatcacgac aatcataagg ataatcataa tattgtttaa tcaaaacctt gaatatatcg
    42421 acacaggaaa ccagggagct tttgatatcc tctggtaact gattgttttt aaccgtatta
    42481 agtatgcttt gttgcgtata tggcatcaaa ttattgtgcg gagcttttaa tgatatcatc
    42541 gcgatctctc ttaaataaaa ataaacgtcg cagataataa agctctcatt aaaccaggca
    42601 ctatcagaaa tcgctcataa ctaacgtgtg aagtattgtg tactggaggg agttaattta
    42661 acaatttgaa tttacgttat aggttttact gctactttga tattatttcg ctctccttaa
    42721 ctaaaaagtt aagtggagag cgaagttaat atgttaaacc acagcgtatt tattaatttt
    42781 ttcagtctgt tagtgtgcga ttatcgataa caaaacgata tttcacatca ccacgcagca
    42841 ttcgctcata ggcttcatta atttgatcgg cccgaatcat ctctatatca gccacgatgc
    42901 catgttcggc gcaaaaatcg agcatctcct gagtttctgg aatgccgcca atcatagaac
    42961 cggctatcgc acggcgtttc atgatcaggt tgaaaacttc cggcgattta tgcggtgtcg
    43021 caggcgcacc aaccagcgtc atggtgccat cacgcttcag caaggtggta aaatcgtcga
    43081 gattatgtgg ctcagctact gtattcaaaa tgaaatcgaa actcttcaca tgagccgcca
    43141 tctcatcggc attgcgtgag ttaacaactt catcggcccc cagggctttt gccgcttcgc
    43201 gttttgcctc agaagtggta aatgccacca catgtgcccc catcgcgtgg gccagcttaa
    43261 tccccatatg tcccaggccg ccgatgccga ccacgcccac ttttttaccc ggcccggcct
    43321 gccagtgacg tagcggcgaa tacgtggtga tccctgcaca caacaaagga gccaccgccg
    43381 ccagctgctc ttgcgggtga cgaatacgca gaacatatcg ctcatgaacg acgatctgtt
    43441 gtgagtagcc gcccagagta tggcccggtt cgtccggcgt cggcgagtta taggtgccgg
    43501 tcatgtgatc acagtagttt tccagcccgt cttcacactc ttcgcaatgt ttacaactgt
    43561 cgacaatgca gccgacaccg accagatcgc ccggcgcatg tttttctacc tgatcaccaa
    43621 cggctaccac acgccccaca atttcatgac ccggtacgca ggggtaaacc gtccccgccc
    43681 actcggaacg gacctggtgg agatcggaat ggcagacgcc acagtaagcg atttcgattt
    43741 tgacatcatt cggtcccggt tcacgccgtg tgatatccat cggttcaagt ggttgtttag
    43801 cggaatatgc accaacagct ttgatcttca ttgtgtttac tcctgattag ctatgtgtat
    43861 ttggcgatgc ctgatatgtc atcgcggcaa aacgcgtcca ttgaatatag ccaatattta
    43921 aaccacccga tccagcccca acatgccgcg aacctgaatg aaacagagca gcaacgttcc
    43981 ccataaggcc atcgtcaggt aggggcttat accgagaata ttgaagccgc tttcaataat
    44041 ttgcagaaga aacagcgcca gcaccatacc aattatccgc ccgctgccgc cgtccggatt
    44101 aaccccgccg agtactgccg cgaggattga caccagcaag tacgattcgc cataagaggc
    44161 cttagccgag ttgagtttcg acatcatcaa aaatgccgct accgcacaaa gcaaggcgga
    44221 aatgacataa acccaaatca gcacccgacg agtgttaatg ccggagtaaa gtgttgctcg
    44281 ttcgttagac ccaatcaggt aaatggcttt accgagcggc gttttctcca gcaatatcca
    44341 cagccccagt gccactgcgg caaacaacca catcggtagc ggaataccga accattgcgc
    44401 atgattcaac cacagcaccc atgagggata attggcaatg gcgctaccac ctgtcaccag
    44461 aatatttatc cctttgagca acgtcatcat cccaagcgtt gcaagaattg gcgacactcg
    44521 aataccagca atcaatacgc cgttacataa accgattatc accgccgcgc cagcgcccgc
    44581 cagtaacgta gcgacaaccg tagcaatacc cggtggatac tgagtcgcca cccacgccat
    44641 caccagtgaa caggcgttag cagtggctat aattgacaag ttaatgccac cgcaaagcat
    44701 ggtcaccgcc atagccaacg ccagaatgcc cagcacgggc atttgcgaag cgacggactg
    44761 gaaattactt atcgaccaga aaatctccgg catagtaatg ctgaacgcgg caacagttag
    44821 caccagcagg ccgatcaagt aaaactcaac gttattacgc cacgattttt tcatagcaga
    44881 ctccggttct gatgctgact ccacgccgtg gcactaatgc tggcaacgat gatgatgccg
    44941 gtgatcaatg tttgccagta agacgagact cccaataaat ttagcccgtt ttgcatcact
    45001 gccaacaaca ccacgccgag caaagtaccg gttaacgtgc cgcgcccgcc gagcaaacta
    45061 gtgccgccaa gcaccaccgc agccagtact gtcagctcat aacccagaag agaatcgggg
    45121 gcggcagtca tcaccgtcca cgactgcact acacccgcag cgccagacat caatcccata
    45181 tagccgtaga cgaaaagttg cagtttcagc acgctaaagc cgatgcgact ggctgattcg
    45241 cggttcccgc caagggcata aattttgcgc cctacactgg tgaaattcat gataaacgct
    45301 gtcagcagca ccaccgtaat catggcgatc agcggcagac caaggccata gtcatagcca
    45361 tcagcatcgg tgtacttgaa caacataacc ccctgctcaa accacggcgg aaagtcgtac
    45421 agccacacac ctttactcaa ccataacagc aggccataga aaatattcag cgtcgagata
    45481 gtgatgatga tagaaggcac ccgtaggcga ttaaccagta aggcgttaat taagccaagt
    45541 aaaatgccga tgcagcccgc cagtgcaaac gcgacagcga agttgccacc aattttttgc
    45601 agcaacagca ccatgccgta ttgcgcaatg atggtcattg ctggaaacga aatatcaatt
    45661 ccaccggaaa tcaacaccac aaacaaacca caggcgagaa tagtcaacat ggcatagtta
    45721 ttggcgagat cgtacaaatt accgaatgtc agaaactcgt cgctgcgcca ggcaaggcca
    45781 acaaacagca ccacaatcag taatgccagc cagaattcat ggcgtttttt cagttcagcc
    45841 atttaccacc tccgcaatcc tggcctgaga actgctgtca ggcaggaagc tatgggtgat
    45901 ctgacctttt tgcattacca aaatccggtg gctttgatac cacgcttctt caatttcatc
    45961 gcagatcatc aataccgcaa tgccatgagc cgccagatcg ctgataatgc cgtatattcc
    46021 tgctttgttg gcgatatcaa caccgactgt cgggctatcg agaatcaaca gtcgtggccc
    46081 aatcgccagc catttggcga tcgatactcg ctgcgcgtta ccaccagaaa gcgtattcac
    46141 cggaagatga ggatcagaga ctttgatggt caatgccttg attagcctgt ccaccaggtc
    46201 acgaacttta gtttcactca gaaagccgaa gcgatctttt accttgtgaa aaacagtgct
    46261 aatgatgttg tcttcaatag attgcgccat caccaaaccg cgcgacatgc gatcttccga
    46321 aacataacca ataccgtggc ggatagcgtc ctggttacta tgcagtgtaa ccggctggcc
    46381 gttgatgagg atctcgccag catccggtcg ggtcatacca aacagactca gacataattc
    46441 cgtgcgcccc gcaccaagca ggccaacaat cgataccact tcgcccgctt ccacccgcaa
    46501 gttaatattt agatattccc catgacgact taaattacgc acctcaagca ccggggtgct
    46561 ggattttccc tgccacaact ctcgcacctg atattcgaac tcctggccgg tcatcaggaa
    46621 accgagttgt tttgtggtca tctctgcagc cggaaaagtc ccgaccagct caccatcttt
    46681 cagcactgaa atacggtcgg aaacttccat cacttcttcc agacgatgac tgacgaagac
    46741 cacgcagatc ccgcgttccc gcaattgatg caccacctgt aataaccctt gcacttcctg
    46801 atgggtcagc gaagcggttg gttcatccat cacgatcaac cgtgcgtcct gcgccagtgc
    46861 acggcaaatt gctaccagct gacagcgggc aattgaaagc tcagaaacga gcgtatccag
    46921 tggcaatgtg acgttaatac tggtcattgc cgcctgcgcc acctcacgca gacgacggcg
    46981 gttaacaaac agaccgtggt gatagtgatt cacggcgata ttctcccaga cactcaggtt
    47041 aggaaataaa gagaggtcct gataaatcac ctgaatcccc tgcgccaccg aagcggcggg
    47101 tgtcagcttt gaccagtttt tcccaccaag cgtaatttcc gctccctcat ccgggcgata
    47161 aacaccggac ataattttaa tcaacgtcga tttaccacag ccgttttgcc cggcaaggca
    47221 gtgcacttca cccgacataa agtcgagaga aacatcgcgc agcgctcgcg tggcatgaaa
    47281 cgttttgttg atatgacgaa gggaaaggaa ggtttccata acatcccctg gcaataagaa
    47341 tcggggcttt cgccccgaag aaatggatta ataaagtgag tcgatattgt ctttgttgac
    47401 cagtaatacc ttatggaaac ggataattcg cttatccata tctacgtcgg ccttacccag
    47461 attctgcatt tctaatccag gtttgatctc ttcacccttc agcaacgtgc tggcgacggc
    47521 tgcgagtgcg taacctgcag aggccggatc gtaagtaatc ccttcggtga tatcaccact
    47581 tttaatcagt gatgccgcct gtgaagggat catcatgcca tagactgcga ctttattttt
    47641 cgcccgtttc tctttcaccg cacgtcccgc gccaatcgga ccgtttgaac caaaggagac
    47701 aaccgctttc aagtcaggat aggttttcat caggtccagt gtggtacgac gtgagtcatc
    47761 cacactctcg gcaaccggca tgcggcgggt aacttcatgc atatccgggt aatgctcttt
    47821 ctggtatttc accagcaagt cagcccataa gttatgctgc ggcacggtca aactacccac
    47881 gtaaatcaca tagccgccct tgccccccat gcgtttcgcc atatgctcaa catattcagc
    47941 ggcaaatttt tcgttatcaa tgatttcgat atcccagtta gcacttggct gaccggggga
    48001 ttcgttggtc agaaccacaa ttccggcatc tcgcgctttt ttgaataccg gttccagcac
    48061 gttggcatcg tttggcacga tagtaattgc attaacctta cgggcgatta aatcctcaat
    48121 aattttaact tgttgcggag catcagtact tgaaggcccc acctgtgacg cattaacacc
    48181 aaaggcgtta cccgcctcaa ccacaccttc gcccatgcga ttaaaccacg gcataccatc
    48241 gactttagag atattcacca cgactttttc cgctgcctgg agcggcgcag aaattagcgc
    48301 agcgcctaat aacagcgaag acaccatatt gataacaaaa cgtttattca tcatttcagc
    48361 ctcattgcag ggtagtcagg aagtgttcgc ccaaattagc gcgctggaga gaataagaat
    48421 tttcgaatat ttttatgcag aataaacagc cctatttcag gcagctatta aaaatatggt
    48481 tataaatatg gacaaaacgg gcagcaacaa taagtgtgac attcatcacg taaaaaatag
    48541 caaaataaac aaaattagcg atattttatg tgtgtgagtt tatttcgcgt agataaatag
    48601 ttatctacac aacgtcaata agggcatatt cttcaaatat aacgtccatt tctggtgaat
    48661 agcttccttc tgcctggaaa aaacgacggt gttcattacg ccaatattca agactgaggt
    48721 caccttcacc ttccttacgg gcaagctcca cggtgacatc agaaaagcgc agtaaatgca
    48781 ggccaatcac tctgataaca catacaggct caccacggct attttctaca atcttatact
    48841 caccaatcat cggaaatgcg ttgtcttcaa tacatcccgc cagcgaacca caggaagctg
    48901 ttttaacacc agtcgcaatc aacgttgcga ggtgatcggc caacgctgct gaatcgccaa
    48961 aagtacaaaa ggaagcatgg ggatattttt gtgagaattt ttcttttatg gtcatggcaa
    49021 tatttttgcg attacggaaa aacaaatcac atcttaatta ctgttggtaa ccatcagcaa
    49081 gagtatcatt ggtttttcga tactgaatct tttttatccg aagcaggtca catttatggc
    49141 ctgataaata cctttgttaa aactcacttt tgtgtatttc tctcaaataa tcccctggaa
    49201 attatgccgc gagattataa agttcaggat cattcattag caaaccattc ccgccccact
    49261 ccctggggct taaatctctt acaattcacc gttgcaggcg tttcaaatgc gcatcaacct
    49321 gccacactcc cccacaccag ttgcccttta tggaacgttg cggttctcgg cgagatacgc
    49381 gccaccgcct cggcggaaca ggaggcgtcc accagcacaa agctggcatc atcctgcgct
    49441 tttggccata cacgctcgcc tttttcattc agcggcaata catcgccagt ggctagaaac
    49501 agcgaacggg agaggttctg ttcgttagga cgaatataga gctgcgcgta gagattggct
    49561 ttttccagca tgtcgcccag accataaggc gaccagtggt cgataacgct gtcagtgcca
    49621 gtcatcactt ttacgccttt gtcgtgcaac tgtttgagcg gcatatgcag cgtgccaatc
    49681 ggcaccgtcg aggcgataga aatttgctgc gccgccatcc gattcgccag cgcatcgacc
    49741 tgttgttcgt tgagcgtagc cagcgcaaag gcgtggctga tggtcagctt acctttcagt
    49801 tgcggcgttt tctctaccgt ttcaaccata taattgatgg ctgccacgcc cgacggagtg
    49861 gtttcgtgca ggtgaatatc gacacctttg tcgtagtcca gcgcaatctg aaacatggtg
    49921 tcgagggatt tttccatcgc gccatcgaca ctggtcgggt ccagcccgcc gacgtaatgc
    49981 gcacccgcct gcatcgcttc gcgcattaag ggttccgatt tcgacagcag caaaccgtgc
    50041 tgcgggaagg cgacgatttc acactcgaag cccgcctgac gtcgcgccag caccgcctgc
    50101 aaattctgca gatttttcag gccggaaacc ggttcgatat tgcagtggct gcgggcaatg
    50161 gtggtgcctt tcgactgcaa tagatcgatc agtttttctg cccgttcctg cgtgtacggt
    50221 tgcagttccg gcagcatttt ctgctcaagc ttgatcatgt cctggatagt ggtgcctgcc
    50281 ggacgattga gcgagcgcca cggcccgccg tagaaggttt tgtcgagatg aatatgcatg
    50341 tcgcgagtgg tgggtagcat caacttaccg ccagcgtcat agtgcggcag cgtggcgtcc
    50401 ggatgctgct tgttctcgcg cagggcgaca attttgccgt cctgaatctc cacggtctgg
    50461 cgcgccgtgc gggtctgcac cgccacaccg ttttcgtagt caaaaccggt ttccagcagc
    50521 acgttatcga gatagtaatg cgggtcagtg attttcatgg tgttgttcgc ttcgcagttt
    50581 agggtgccag ctaccgccgc atgggcgagc ggcacagagg taccaaacag cgcggcggcg
    50641 gtgaccatct taccgctctg gctcagaaat tcacggcggc tattgctttc tttcatccta
    50701 cgtccttacg ttttaataat atgggtgccc gtttcaccgc gcagcgccgc aggcaggcat
    50761 tccggcgtgg tgataatcac ttctttgccg ccctgctcca ggaacgtcag gctggcgatg
    50821 atttttggca acatgctgcc gggcggaaaa tgcccttcct gcatatagcg agtcatggtg
    50881 gcaatatcca cccgatccag cgcctgctgc tgcggtttac caaagtggat acagactttt
    50941 tcgacgccgg tggtgatcac cagaatgtcg gcgtgaattt cacgggccag cagcgcggta
    51001 gagagatctt tgtcaataac cgcgtccacg ctttggtaat cccccgcttc agtacgcact
    51061 accggaattc caccgccgcc cgcgccaatt acgacaaaac cttgttggat cagcgcctta
    51121 atggcgggtg cttcgacaat acgtttcggt tccggcgagg cgaccacgcg gcgatagccc
    51181 cgtccgacat cttcaacaaa acaccagtca gggtttgcct tttgtagttt gtcacgctgg
    51241 ctttcactga agaatgcacc aatgggcttg gtgggatggg caaaacctgg atcgttttta
    51301 tccacttcca cctgagttac cacggtgacg gcttttttct cgccgtgacg cgccagccgg
    51361 ttattcagcg cctgttggat cagatagccg atgccgccct gcgtatctgc cacacagttc
    51421 gccagcggcg ttaagggcag cccttcgcgc tcgtgggcaa tctccgcacg gcgtaaatcc
    51481 agcccgacct gcggcccgtt accgtgggtc agcacaatgt cgtaatcgga agccagcatt
    51541 tccagcaccg tatcggccac ggctttcacc gcctccgcct gatgctcaat cgactggctg
    51601 gcgttatctt tgataatgct gttgccacca atggcaacga ccacaagctc tttcatgttg
    51661 ttatgtccgc tacagagggt gattaggtta tgaccaacgc cagactgcct gatgcgctac
    51721 gcttatcagt atctacaatt ctcgggccat ccgtaggccg gataaggcgt tcacgccgca
    51781 tccggcatga acatgtaggt cagacgccgt aacgttcgca ccagccaaga atggctttct
    51841 cgaagcaggc aaaaggtgga tgcacaatgc ccgcgccaat catgccgacg cccgcatctt
    51901 tatgggcgat ggcagtgttg atcaccggca gaatgccgct gctacccacg cgggtgatgt
    51961 cgattgccga cggcacgccc ataaaaccga gcagcggaat ggtgacgttg gggttttcac
    52021 cgagagtgat ttcacgcatc tggcgagaga aatcgatagc ctcttccacc gtgccgccca
    52081 ccagcgcaac gattgcaggc gcggtcgcca tagcaaatcc gccaataccg taggtttcag
    52141 tgatggcgct gtcgccgata tccagccccg aatcttccgg cttataaccg gcaaacatcg
    52201 ggccgatgac ctgctgcgcc ggaccggtaa accattgccc cggtaatccg ctgacccgca
    52261 ggccgaactc gacgccgtta cgcgccatgg tggtgaccac ggtgctgtat tcgatgccgt
    52321 gcgccgcatc catcgccgct ttacacatcg ccatccacgt cgggccggag aagtagtcgc
    52381 tgctagcgac aaactcaaac acttcgcgct gtttctccac ggaataacct gcctgaataa
    52441 ttcccggcgt cagcgcctga atcagcaacg tcgtcccggc gttattgcgg ttatggcact
    52501 catcgcccat atgcagcgcc tgcgccagca ttaagcgcag atcaatctcg ccgataattt
    52561 tcatcgcgtc gcgcagcatt ggcccctgca catcgcgcat ccagttgagg cggtcgatca
    52621 cgctctgatc gttggctccc atacgcaaaa tcttcgccat ctgctcgctc atattggtgt
    52681 aagcgatgtt gccgtaggtt ttgtttttta cgatgtgcat aaacatcgag gcagaggtaa
    52741 cacccgccat cgatcccaca cagtcgtgtt cgtggcacgg cgagaaggtg atctccccgg
    52801 aggccgccag ttcagccgcc tcgtcgagat ctttcgccag cccttcgaac accaacgccc
    52861 cggtgaccgc gcctttcatc gcgccgcaca ttttttccca ggtgatcggc ggcccggcgt
    52921 gaagaatggt tttcggcgtc atgcccggca ccacgttaat cgcctgatca aaaccaatca
    52981 gcaccggatg cgactggata atgcgctcca gcgcctgctg gttagcggcg gcgattttgt
    53041 ctgccagcgg cgaatcggca atgttatcca gcgcctgcac cacctgcata ttgccctgcc
    53101 ccggcggcgt ccagtcgagt tgagtcactt ctacatgctg ctttttcagg tcatcgctaa
    53161 acatggcgat gccgacgtta ataacgttca atggttggct aaacagtgac tggctcatca
    53221 ggcttcctcc cctttgcaga taaattcacg cgccagcaat ccggtattgg tgctgctgct
    53281 cgccagaatc actccggcat caagcagcat ctggctttgt tgttctaacg atggcgtatc
    53341 aagatcggta cccagcacat aggcgagaat gatcaactcg cgtccttcgg cagcggcgat
    53401 cgctttcgct tctttgatcg cctcgatggt ggagccgacc ggatcttcat gcgatccaaa
    53461 tccgagcaca aaatccatca cgatcaccgc cacttctgga tcgcgcgcct cttcgatcaa
    53521 gcgactgatg cggttggtgg ggtcaatcat tggatgcggc ttgccgttcg tgaagtcgtc
    53581 atcgccaaag tcgaggaagg tgtgtttgat gctgcggttg atatcttgca ggcggaattc
    53641 cggatcgggc ggaatgttgc tgtagacatc gccatgtttt tccatcaccg cgaacatggt
    53701 ttcgtcgcac agcgtgccgc cgcagaacag gccacgaatg tatttctgct gcggttgcag
    53761 acgcgcacgc acatcagcaa tcaacggctg gttaagcgta tgcaggtcga gattttcctg
    53821 tttcacgccg gagagcatca ccgcttttag cgccgcctct ttgctgccgc gggcaaactg
    53881 tagcccctgc tcatccaccg gcgtttcaac gcgaccgagg aagcagacca ccaccggctt
    53941 gcggcaggca cgtgcgcgtt ccagcacttt gcgggccacc gcaggcgcgg gaggttttga
    54001 aatcagtgcg ataatttcag tttgcggatc gttttccagc atcccgatgg cgtcgagcat
    54061 catcaggccg ccgattttct cgctcaggtc gcgcccgccg gtgccaatca gttgtgaaac
    54121 accgccacca aattcatgaa tgcggacgct cagctcctgg ctgccggtgc cggatgcgcc
    54181 aacaataccg atattgccgc gacgcacggc gttaccaaaa cagagcgccg cgccgttaat
    54241 aatcgccgtg ccgcagtccg gccccatcat cagcagccct ttttcgtggg ccaactgctt
    54301 gagcgccagt tcatcttcaa ctgagacgtt atcggaaaac agcatcacgt tgagatcgtt
    54361 ttgcagcgcc tgacgcgctt cgcgagcggc aaacagaccg ttgaccgaaa tcaccgccag
    54421 gttactttcc gggatatgct ttttggcgct ggcaatagtg gcgtaacgcg cctcgtgcga
    54481 gccgctttgc gctttggtgt tgaacagttc ttcaatctcc accagcaact gctcgttgtc
    54541 cgcacccgat ttaccattga tgacaatcat caggtcgccg tttttcgcct gttccagctc
    54601 cggcgtcagc agtcctaaat ttttcagtac acctttgttc atttcggtcg ccatcgccac
    54661 aaatgcctgc tcgacgccgt cgagtttatt tgcacgcgtg gagatagaca tcagcgagac
    54721 agaatcaaaa taggtattcg gtttaatgac tattttaact gacataattc cctccgtagg
    54781 tttggctcag cgcacaacca tcggccatgc catacagcat gtcgcagccg gaactggagc
    54841 caatatgttt aattttttct attgcctgag ttgcgttccc agggatgtca taaataatgt
    54901 tgtgaataaa acgatgaata ttttcccggc aacgttgttg taatgcggct tccagcgtta
    54961 tagcacttaa taatgtggtg ttatttctgc cgcgctgcat accgagataa aattcctctt
    55021 tgtatttttc cgacggatgc ccgggaataa ataaaataag cgccagacct gttaaataat
    55081 cgtcggctga tggcgtaagg ccaatcccta acccaatcat actggcgacc gctgcggcga
    55141 tattttgttt ttcgtttact gcctgaataa ctgcccgtcg tttaatatgt agttctttat
    55201 ttaacgcctg ataaaacgga ttatcgccgc gataattaaa cagggtttca ttttgcccca
    55261 ggtgctgatg aataatgtcg tgccactgta gccaggcgat aaggtgaaaa ttcacagcgg
    55321 tcatttgcaa ggttggggac agccaacgcc gacaacgact catctctata tgaagatgtt
    55381 gaccaaccgt aataccttga tcgtcaaact gaaccttatc accatgccgg aacagatcat
    55441 caaagtgagt gagtgccaac cgacaactgt ttggcgcatt gtcgtactct tcacaaagca
    55501 gcgtcagcaa ctgctggcga gccgggagtg cgatatttac cgctctggca aagacctgtt
    55561 caacctgtcc ttgcccccgc tgcgccagaa aatccgcatc cgcagttagc gcccacacac
    55621 accgtctttc tggtgtgttg atagcaagcc tcccggttta tgcacctgcg gcaatcagta
    55681 actgcgcaat ctcttcaaag ccccgttccc gcgccagttc cagtggcgtt ttgccatatt
    55741 tatcggtcag atgcgggctg gcaccgtgtt ccagcaataa ctgcacaatc gcctgctgtt
    55801 taataccacc atcattaagc acaatcgctt ccagtagcgg cgtccagcct acatggttgg
    55861 tctggttaac gttaatctcc gtatgcgcca aaagctcttt tacaatactt aaatggcctt
    55921 tttcacaggc aggcgtcagg ccgacaccgc caaaacgggt tacgcaatta agatcgggtt
    55981 tagccggtaa aataattcgt agtagcgtta aatcatcgtt cagacagcta attaaaaaag
    56041 gatttaaaca ggtatgatct tgcttattaa tatccgctcc ggcatcaatt aatgcctgaa
    56101 cgcaagcata ttgctgatat aaacttgcca gcgtaattgt cgttttcccc tgacgatcgc
    56161 aggtattaat atcgacaccc agcgcaaagc aggtttttac tttatcgata tcaccctgtt
    56221 gtgcagccaa taaatagtcg gcaggtagat tttttatact cattcacatt cccctggttt
    56281 ttgtggctgt tttcgatgaa ttgagaatag caacgataat ctgataaaag aacccgctta
    56341 aatttaattc gctgtatttc gcctttttgt tgaatgatgt tcaacaatct tttcgcagtg
    56401 tgcggtagtg caatatttgt ttctttgacc cgataccctt tcactcaatc aggttttatt
    56461 tctctatagt ctgcacatgt aagagtagct ttcgtccatg tctaaccaga ggggaacaag
    56521 atgaacggac tcacggcaac gggtgtcacc gtcggtattt gtgcagggct ttggcagtta
    56581 gtgtcttcac atgtcgggct ctctcaggga tgggaattat taggaaccat tggcttcgtc
    56641 gccttttgca gtttttatgc cgcaggcggc ggtaaatcgg ggtttatcag aagtctggcg
    56701 gttaattatt ccgggatggt gtgggcattt ttcgccgcgc tggctgccgg ttggctggcc
    56761 tctgtaagtg gactgtccgc gttctgggcg agcgtaatta ctaccgtacc cttctcggcg
    56821 gtagtggtct ggcaagggcg tttctggtta ctgtccttta ttcccggtgg ctttctcggc
    56881 atgacgctgt tttttgccag cgggatgaac tggacggtga cgttactggg ttttctggcg
    56941 ggtaactgcg tggggataat ttcagaatat ggtggacaaa aattaagcga agcgacgact
    57001 aaacgcgacg ggtattaaaa gttattcaaa aatgattatc tatctttaac cgatacctct
    57061 acagtcatag ctggagaaat tcatagtaga taatggcgag aatggtttaa tgaactcaat
    57121 ttttaccgaa gagaatctgt tggcatttac taccgctgcg cgttttggca gcttcagtaa
    57181 agccgcagaa gagttgggtt taaccacttc cgccattagc tacaccatca agcgtatgga
    57241 gacggggctg gatgtggtgc tgttcactcg cagtacccgc agcattgagt taacggagtc
    57301 cgggcgttat tttttccgca aagctaccga cctgctgaat gatttttatg ccatcaaacg
    57361 cagtatcgat actatttcgc agggcataga agcgcgtgtg cgcatttgta ttaatcagct
    57421 gctttatacg cccaaacaca ccgcacgctt gttacaggtg ctgaaaaagc agtttcctac
    57481 ctgccagatt accgtcacca ccgaagtgta taacggcgtc tgggatgcga ttattaataa
    57541 tcaggccaat atcgccatcg gcgcacccga tacactactg gacggcggcg gtattgatta
    57601 cactgaaatt ggcgcgattc gctgggcttt tgctatcgcc ccggatcacc cgttagcctt
    57661 tgtgccggaa cccatcgccg aaagccaact gcgtctctac cctaatatta tggtggagga
    57721 caccgcgcat acgattaata aaaaagtggg ctggttgctg cacgggcagg agtcaattct
    57781 ggtgccagat ttcaacacca aatgtcagtg ccagatttta ggtgaaggaa ttggtttttt
    57841 gccggattac atggtccgtg aggcgatgac gcaatccctg ctggttaccc gacaaatcca
    57901 taatccgcgc caggattcgc gcatgttact ggcgactcag cattctgcta cgggtcaggt
    57961 gacgcagtgg attaaaaaac aatttgcgcc aaatggcata ttaacgggga tctatcagga
    58021 tttactgcat cgggagaatt aatcgctgcc aacctcccga tgcgctgtga atagcggttt
    58081 acatcaacca cctgctttca ttacccattc agagataaat ttattacccg gtactggcgg
    58141 agagtacaaa tagccctgca aaaagtgaac gcctttaccg atcattaaat ccgcctgctc
    58201 ctgggtttct accccttccg ccacgatact cagaccaggc ttacgcgcta gttcgacaat
    58261 attgtccaca atatgaccgg agatttcgtc gacactcgcc atttgcacaa atgacttatc
    58321 gatcttaata aaatcgaccg ggaacgcctg caagtaacga taggtcgcat aacccgtacc
    58381 aaagtcatcc agcgcaaagg taatgttgtg ctgatgaagg ctgtcaaata tcgctctggc
    58441 ttctggcgtt accggaatag ggttacgttc cgttagctcg agaaccagct tgattttatc
    58501 gttacctaat ttcttaacca ggttcagaca ctctttttca aatcccgctg ccaaaaaaca
    58561 acccgccgag acgttgatgc caatatggaa attgtccggc agcaaatgtt ttaccggcat
    58621 cagaatatcc gcagtctgtt tcatcagttg gcgggtcatt atgacaataa gaccggatga
    58681 ctccgccaga ggaataaact gatccggtgg gataattccc gtttgtggat gttcccagcg
    58741 gacaagtacc tcacagcccg tcagtacccc agtctgcgcg cagaaaaccg gttggatcca
    58801 cggtttgaat tcgtggtttt ccaacgccag actgatggct tcaggcgtga cgatatggtg
    58861 acaacggtta tcagggatat aaaaattatt tttatttccc gtgccactga tgacttgcgg
    58921 atgatattga aaaaagatat cccgccagaa ggtaatatca ctctggatgc ctagtttttc
    58981 atacagctgc ttcttttgat gtgaaattgt ctttgcactg cgatttctgt actgtgagat
    59041 ttctgtaacc gacattccct ccattagcag cttgagtaaa agccgttctt tttcggacac
    59101 gataccaggc aacgtcaaat tcaccgttgc cccgaactct tccaaaaaaa cactcaaatc
    59161 atgcgaagcc attgaacaca ccttgattaa atcttgtgcg ccagctaaat ctgcgagtct
    59221 tcactctgac tgataaagct ggctgcaaga aaatgacaca gagagccgaa ttatctcccg
    59281 ttcggctctc tctcctttta ctcattattc agatcagaat tgccacttaa tgccaaccat
    59341 tgcagaggtg tcattatatc ccctatcggc aacctgaacg cccacattcc cccagacatt
    59401 caggttggca tttagctgtc cttccacccc ggtttttatc tcagcaatat ttcgggctcc
    59461 atcctgagtg acggacacgc catccatact ggtgctgaaa tccttactgt tatgcagcca
    59521 gttcacttct acaaacggct ggaactcgcg ggatttaccg tcatccattt tatggtggct
    59581 cttcagccag gtttttacgc caagtcgggt ttgaacattg ccatcaccgt tgctatgaac
    59641 gagggttccg ttgctttcgc ggtgcttatc ggctttgact cccatccagg taacctgtgc
    59701 ttgcggctga acataccatt cattacgcgt tccctggctg ccattaaatt cagctaattt
    59761 gtgtttgtat ccagcttcca gtgaagcggt aaatcctttt gatttatagg attcactttg
    59821 taagtcatcc cctttcactg tgttatcaaa ccagctgtac tgcgcccaac tgtcgagata
    59881 cgcgccatta cgcgactcgt catcggcata ccaggtggca tagaggccgg tgctatatcc
    59941 gttcacactc gcttttgcac gataaccggt tcgcgaggaa atggttttgc tgtcgctgtt
    60001 gccatatccc gccatgaccc caacatgcca gctgtcgctg ccgttttggc tccactgcgc
    60061 gacatcgcct cccagttgca gaacatagcg attgctttgg gttttcagct ggccgctgcc
    60121 atcacgccat ttattatgac caccttcatg gcgcatccac atagtggttt gtttctgctc
    60181 acccgtcacc atgtcggtat agtacgtatt acccagacgc tcatgcagac gcgtggtgaa
    60241 cattgtattc gctgctgcaa ggttcgcaat gtagctaccc gcctccggtc gcaggtcatt
    60301 atccacattc agatccggac ccggcgtagg tgtcgggtta gggttcggct ctggattcgg
    60361 gtttggctcc ggattcggca tcgggtctgg ctccggctgc agttcaggag aatcactgcc
    60421 gctggtcaga taccagttac cactatttgc cccttgtcca cgcgcgagag tgtagtcata
    60481 cgcccccgca acgatacgac cagcctgaac aaattcgccc tcagacttac cgtctacatg
    60541 gataagttcg ataccgttaa gggttttcgc acctgtaccg ccagcgttat tcaccgtaac
    60601 ggcagtcgtg ccgctagtat cgccctcgac aaccaattta tcggttactg agtcatcgcc
    60661 attcagtacc gtattcatta ctagttgacc gttattaccg tggtagttcc ccttcaccac
    60721 taacacattg ccagcttgct gtcctgacat tccggtcatc agcgtaccgt tgttggtcag
    60781 attgccgccc agggtgtaat tcacagacgc agccgctctt ttttcagtgc tattattcaa
    60841 cgccagggta ccgttgttct caacgtttgc gctacccaat ccctgcgcat gacttaccag
    60901 gacattagcc gaattagcaa tgttcacatt cgccgtcagg ttgctgttat cgcccgcgat
    60961 gtccagcgtg ccgccctgaa tattcagctc tccgctcccc gttaacgaac cggttgaagt
    61021 gcccccgttg gtaaccgtca gactgccgcc atttaagctc agcagtgaat cggcgctgcc
    61081 attgagcgtg cccacggtct ggctgtgacc attcatatcc agttcagtct ctgccgccag
    61141 acgcagttca tgggtattac ccaacacgtt atcgttattc aacaaaagcg tcccactgcg
    61201 cagatcggta acaccggtat agtcgttgtc tttgttagaa agcgatacgg tctgaccctt
    61261 ctggctgctg aatgccaggt caccactgcc ggtaatcttt gcgctgagat cggcggcatt
    61321 ctcgctttta ccgttagagc tcagcaccag agcatcgctg tcggtagcgt gtaaatcaag
    61381 ctgggtcagg ccgtaaccga tatacagacc gtcattgttt accccactcg tcagacgata
    61441 gtcgtaagtg ccgacggccg ctttttgccc attctgctga atatcacgtt gggcgctgct
    61501 ggagaggatt tgcccatttt tatcctgcaa ttgcagattg cccgcatcgc ccagaacttc
    61561 cgcaccttgc gcatcaacca gcttaatggc gctgttacca tcatcgactt cagtgagcga
    61621 taacgcagag tcaatatcac gagatactga gcggacaacg tcactgtcag aaacctgaat
    61681 cacaccctcg ccgcgcaaat ccagcgtttt actaacgttg accgtccctt ctgtcatctg
    61741 tgcacctgct gtcagggcac caaaatcaac ggttccgccg gagaatccca aagtgctaat
    61801 ggagtgagcg cccttaccca ctgttacctg gcttccctga cccgcagtga tcccgcctct
    61861 ctgcagaaac tcagcattct tttccaggtt aaatgtcgtg ttctggaacg ccagtgaacc
    61921 ctggaaccag tctgtataag caaactcatc gaaggagaat tcgtgattcc ccatgtttat
    61981 ttcaacaata cctctacctt gaagatcgtt atcaaatacc caatctttat tggcattgat
    62041 ttgcagtttg ccatcaacat ccaggtccgc acggccgatg ttcttgtctt cagaaataac
    62101 cgcgacagca ccagaagcga catcaatata tccgctgaat gcatagttat tgccggtcag
    62161 tgccactgtc ccactgtcga tacttattgt accattgccg gaaatcttat tggcaaaggt
    62221 catgctatcg tcagccttca ggtgtaattt cccgcggttg ttgatctctg ctgtacctat
    62281 atttttggtg atccctaaag cgccattttg ctcaattttg actttaccac taaaggcaga
    62341 gttatcaccg gttaagtaag tcgtgccatt gagcgcattg atatagccca ggccctcgct
    62401 gccactgaca atatccgcgg aaatagtcgc gtttttatcc gtgtggttta aggtgatttt
    62461 cttatcggat gtctttgccc acacaaactc aattttttcg gcatcaataa tccctgcagc
    62521 cttcgcagca cttccttcct gtgcacctaa cgctaactca gagttggtgc ttaaactaat
    62581 tttcggtgcg gatattttac ctccatctga tacgactaat gttgtatcag aacgttggtc
    62641 gtaataaccc aggctaatat gttcacgagc attaacttca ccaccgttat taaccgacag
    62701 gaactgatta ccgccatatc cgccgtaaac gcccataaag aaggtccctg catttacctg
    62761 actaccttcg ccgcttacat tcccctgcgt ataagtcaga cgactggtgc ccttaacatg
    62821 aattttgccg ccattggaga cattaaaata gccttgatca tgtctcatgc ctgagtttga
    62881 ctcatacaaa tcatcagcat ataactcgct gccagcattg gagacattca ggaaccccgt
    62941 acctttcagc aaaaagttaa gcgggccatt gattgttacc tttcctccgg taatatccaa
    63001 cccaccacca gaagcgagag tcagacgcgg acgggaagta tctgtcgtat tgattaccag
    63061 attggcattg tcagtcaccg taatacggta atcgatgctc tggttctctt tgccaaaatc
    63121 aatagtttta tcctggttta cgatgacatc atcggcgaat accagagatg aatataaact
    63181 tatcgcgcta attaaaacag cccgggactt ttttcctttt actctgctcc ccagctcaga
    63241 gcagacgctc caacagttac gggacctgtt ccactttagc cgatatattt tattcatcac
    63301 aatctccagc aatacatcca aataaaacca agattgcata aacagcaacg cattaaatgc
    63361 cattctggtt actgttcatc gcatataaaa attcaaaaat tttgcatagc attggcaacc
    63421 acatatctga gtatgtagtt cagagggtaa taaaatagca agttattact taaaacttat
    63481 tttaagttaa cactcttaac tttatcatta cattcctccc agtctctaat acctgcgcat
    63541 tatatgtatc caggaactca aatcaactca aaatataaac aaaagcaag
//
LOCUS       DAZGTR010000015        61413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGTR010000015 DAZGTR010000000
VERSION     DAZGTR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.015"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(72..956)
                     /gene="xapR"
                     /locus_tag="ACHLCY_001069"
     CDS             complement(72..956)
                     /gene="xapR"
                     /locus_tag="ACHLCY_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP0368376.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(997..1155)
                     /locus_tag="ACHLCY_001070"
     CDS             complement(997..1155)
                     /locus_tag="ACHLCY_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFP0368377.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(1208..2464)
                     /gene="xapB"
                     /locus_tag="ACHLCY_001071"
     CDS             complement(1208..2464)
                     /gene="xapB"
                     /locus_tag="ACHLCY_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP0368378.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(2524..3357)
                     /gene="xapA"
                     /locus_tag="ACHLCY_001072"
     CDS             complement(2524..3357)
                     /gene="xapA"
                     /locus_tag="ACHLCY_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP0368379.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            3606..4370
                     /gene="yfeN"
                     /locus_tag="ACHLCY_001073"
     CDS             3606..4370
                     /gene="yfeN"
                     /locus_tag="ACHLCY_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP0368380.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(4409..5335)
                     /gene="yfeR"
                     /locus_tag="ACHLCY_001074"
     CDS             complement(4409..5335)
                     /gene="yfeR"
                     /locus_tag="ACHLCY_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0368381.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            5425..6423
                     /gene="yfeH"
                     /locus_tag="ACHLCY_001075"
     CDS             5425..6423
                     /gene="yfeH"
                     /locus_tag="ACHLCY_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP0368382.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(6420..6638)
                     /gene="ypeB"
                     /locus_tag="ACHLCY_001076"
     CDS             complement(6420..6638)
                     /gene="ypeB"
                     /locus_tag="ACHLCY_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP0368383.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(6640..8655)
                     /gene="ligA"
                     /locus_tag="ACHLCY_001077"
     CDS             complement(6640..8655)
                     /gene="ligA"
                     /locus_tag="ACHLCY_001077"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP0368384.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(8726..9712)
                     /gene="zipA"
                     /locus_tag="ACHLCY_001078"
     CDS             complement(8726..9712)
                     /gene="zipA"
                     /locus_tag="ACHLCY_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP0368385.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            9942..10703
                     /gene="cysZ"
                     /locus_tag="ACHLCY_001079"
     CDS             9942..10703
                     /gene="cysZ"
                     /locus_tag="ACHLCY_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP0368386.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            10888..11859
                     /gene="cysK"
                     /locus_tag="ACHLCY_001080"
     CDS             10888..11859
                     /gene="cysK"
                     /locus_tag="ACHLCY_001080"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP0368387.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            12243..12500
                     /gene="ptsH"
                     /locus_tag="ACHLCY_001081"
     CDS             12243..12500
                     /gene="ptsH"
                     /locus_tag="ACHLCY_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP0368388.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            12545..14272
                     /gene="ptsI"
                     /locus_tag="ACHLCY_001082"
     CDS             12545..14272
                     /gene="ptsI"
                     /locus_tag="ACHLCY_001082"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP0368389.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            14313..14822
                     /gene="crr"
                     /locus_tag="ACHLCY_001083"
     CDS             14313..14822
                     /gene="crr"
                     /locus_tag="ACHLCY_001083"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP0368390.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(14864..15715)
                     /gene="pdxK"
                     /locus_tag="ACHLCY_001084"
                     /gene_synonym="thiJ"
     CDS             complement(14864..15715)
                     /gene="pdxK"
                     /locus_tag="ACHLCY_001084"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP0368391.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            15820..16188
                     /gene="yfeK"
                     /locus_tag="ACHLCY_001085"
     CDS             15820..16188
                     /gene="yfeK"
                     /locus_tag="ACHLCY_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP0368392.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(16191..17102)
                     /gene="cysM"
                     /locus_tag="ACHLCY_001086"
     CDS             complement(16191..17102)
                     /gene="cysM"
                     /locus_tag="ACHLCY_001086"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP0368393.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(17236..18333)
                     /gene="cysA"
                     /locus_tag="ACHLCY_001087"
     CDS             complement(17236..18333)
                     /gene="cysA"
                     /locus_tag="ACHLCY_001087"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP0368394.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIKTRDEELALAQSA"
     gene            complement(18323..19198)
                     /gene="cysW"
                     /locus_tag="ACHLCY_001088"
     CDS             complement(18323..19198)
                     /gene="cysW"
                     /locus_tag="ACHLCY_001088"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP0368395.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQDKRAQQEEHHEH"
     gene            complement(19198..20031)
                     /gene="cysT"
                     /locus_tag="ACHLCY_001089"
     CDS             complement(19198..20031)
                     /gene="cysT"
                     /locus_tag="ACHLCY_001089"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP0368396.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(20031..21047)
                     /gene="cysP"
                     /locus_tag="ACHLCY_001090"
     CDS             complement(20031..21047)
                     /gene="cysP"
                     /locus_tag="ACHLCY_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP0368397.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(21218..22009)
                     /gene="ucpA"
                     /locus_tag="ACHLCY_001091"
     CDS             complement(21218..22009)
                     /gene="ucpA"
                     /locus_tag="ACHLCY_001091"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP0368398.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTDMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(22138..22995)
                     /gene="murR"
                     /locus_tag="ACHLCY_001092"
     CDS             complement(22138..22995)
                     /gene="murR"
                     /locus_tag="ACHLCY_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP0368399.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            23159..24055
                     /gene="murQ"
                     /locus_tag="ACHLCY_001093"
     CDS             23159..24055
                     /gene="murQ"
                     /locus_tag="ACHLCY_001093"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP0368400.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKLGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            24059..25483
                     /gene="murP"
                     /locus_tag="ACHLCY_001094"
     CDS             24059..25483
                     /gene="murP"
                     /locus_tag="ACHLCY_001094"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP0368401.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            25488..26792
                     /gene="pbp4b"
                     /locus_tag="ACHLCY_001095"
     CDS             25488..26792
                     /gene="pbp4b"
                     /locus_tag="ACHLCY_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP0368402.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYDHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(26850..27749)
                     /gene="yfeX"
                     /locus_tag="ACHLCY_001096"
     CDS             complement(26850..27749)
                     /gene="yfeX"
                     /locus_tag="ACHLCY_001096"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP0368403.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGIHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(27845..28402)
                     /gene="yfeY"
                     /locus_tag="ACHLCY_001097"
     CDS             complement(27845..28402)
                     /gene="yfeY"
                     /locus_tag="ACHLCY_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP0368404.1"
                     /translation="MLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQGVGELT
                     ASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQGTISRID
                     VLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKVEGSQHISYQFSGEW
                     SGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(28482..28931)
                     /gene="yfeZ"
                     /locus_tag="ACHLCY_001098"
     CDS             complement(28482..28931)
                     /gene="yfeZ"
                     /locus_tag="ACHLCY_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP0368405.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(28918..29343)
                     /gene="ypeA"
                     /locus_tag="ACHLCY_001099"
     CDS             complement(28918..29343)
                     /gene="ypeA"
                     /locus_tag="ACHLCY_001099"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP0368406.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNSEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            29557..30426
                     /gene="amiA"
                     /locus_tag="ACHLCY_001100"
     CDS             29557..30426
                     /gene="amiA"
                     /locus_tag="ACHLCY_001100"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP0368407.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            30430..31329
                     /gene="hemF"
                     /locus_tag="ACHLCY_001101"
     CDS             30430..31329
                     /gene="hemF"
                     /locus_tag="ACHLCY_001101"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP0368408.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(31335..32387)
                     /gene="eutR"
                     /locus_tag="ACHLCY_001102"
     CDS             complement(31335..32387)
                     /gene="eutR"
                     /locus_tag="ACHLCY_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP0368409.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(32433..32933)
                     /gene="eutK"
                     /locus_tag="ACHLCY_001103"
     CDS             complement(32433..32933)
                     /gene="eutK"
                     /locus_tag="ACHLCY_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP0368410.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(32946..33605)
                     /gene="eutL"
                     /locus_tag="ACHLCY_001104"
     CDS             complement(32946..33605)
                     /gene="eutL"
                     /locus_tag="ACHLCY_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP0368411.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(33615..34502)
                     /gene="eutC"
                     /locus_tag="ACHLCY_001105"
     CDS             complement(33615..34502)
                     /gene="eutC"
                     /locus_tag="ACHLCY_001105"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP0368412.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(34523..35884)
                     /gene="eutB"
                     /locus_tag="ACHLCY_001106"
     CDS             complement(34523..35884)
                     /gene="eutB"
                     /locus_tag="ACHLCY_001106"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP0368413.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RITGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(35896..37299)
                     /gene="eutA"
                     /locus_tag="ACHLCY_001107"
     CDS             complement(35896..37299)
                     /gene="eutA"
                     /locus_tag="ACHLCY_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP0368414.1"
                     /translation="MNTRQLLRVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(37296..38522)
                     /gene="eutH"
                     /locus_tag="ACHLCY_001108"
     CDS             complement(37296..38522)
                     /gene="eutH"
                     /locus_tag="ACHLCY_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP0368415.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(38639..39826)
                     /gene="eutG"
                     /locus_tag="ACHLCY_001109"
     CDS             complement(38639..39826)
                     /gene="eutG"
                     /locus_tag="ACHLCY_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP0368416.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQSRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(39816..40652)
                     /gene="eutJ"
                     /locus_tag="ACHLCY_001110"
     CDS             complement(39816..40652)
                     /gene="eutJ"
                     /locus_tag="ACHLCY_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP0368417.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(40663..42066)
                     /gene="eutE"
                     /locus_tag="ACHLCY_001111"
     CDS             complement(40663..42066)
                     /gene="eutE"
                     /locus_tag="ACHLCY_001111"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP0368418.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(42078..42365)
                     /gene="eutN"
                     /locus_tag="ACHLCY_001112"
     CDS             complement(42078..42365)
                     /gene="eutN"
                     /locus_tag="ACHLCY_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP0368419.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(42472..42765)
                     /gene="eutM"
                     /locus_tag="ACHLCY_001113"
     CDS             complement(42472..42765)
                     /gene="eutM"
                     /locus_tag="ACHLCY_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP0368420.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(42804..43820)
                     /gene="pta"
                     /locus_tag="ACHLCY_001114"
     CDS             complement(42804..43820)
                     /gene="pta"
                     /locus_tag="ACHLCY_001114"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0368421.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(43817..44620)
                     /gene="eutT"
                     /locus_tag="ACHLCY_001115"
     CDS             complement(43817..44620)
                     /gene="eutT"
                     /locus_tag="ACHLCY_001115"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP0368422.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(44617..45318)
                     /gene="eutQ"
                     /locus_tag="ACHLCY_001116"
     CDS             complement(44617..45318)
                     /gene="eutQ"
                     /locus_tag="ACHLCY_001116"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP0368423.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(45293..45772)
                     /gene="eutP"
                     /locus_tag="ACHLCY_001117"
     CDS             complement(45293..45772)
                     /gene="eutP"
                     /locus_tag="ACHLCY_001117"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP0368424.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(45785..46120)
                     /gene="eutS"
                     /locus_tag="ACHLCY_001118"
     CDS             complement(45785..46120)
                     /gene="eutS"
                     /locus_tag="ACHLCY_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP0368425.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(46413..48692)
                     /gene="maeB"
                     /locus_tag="ACHLCY_001119"
     CDS             complement(46413..48692)
                     /gene="maeB"
                     /locus_tag="ACHLCY_001119"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP0368426.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEIVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            48981..49931
                     /gene="tal"
                     /locus_tag="ACHLCY_001120"
     CDS             48981..49931
                     /gene="tal"
                     /locus_tag="ACHLCY_001120"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0368427.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            49951..51954
                     /gene="tkt"
                     /locus_tag="ACHLCY_001121"
     CDS             49951..51954
                     /gene="tkt"
                     /locus_tag="ACHLCY_001121"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0368428.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGVIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(52050..53093)
                     /gene="ypfG"
                     /locus_tag="ACHLCY_001122"
     CDS             complement(52050..53093)
                     /gene="ypfG"
                     /locus_tag="ACHLCY_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP0368429.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(53219..53794)
                     /gene="nudK"
                     /locus_tag="ACHLCY_001123"
     CDS             complement(53219..53794)
                     /gene="nudK"
                     /locus_tag="ACHLCY_001123"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP0368430.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(53862..55841)
                     /gene="aegA"
                     /locus_tag="ACHLCY_001124"
     CDS             complement(53862..55841)
                     /gene="aegA"
                     /locus_tag="ACHLCY_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP0368431.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            56047..57747
                     /gene="narQ"
                     /locus_tag="ACHLCY_001125"
     CDS             56047..57747
                     /gene="narQ"
                     /locus_tag="ACHLCY_001125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP0368432.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            57911..61024
                     /gene="acrD"
                     /locus_tag="ACHLCY_001126"
     CDS             57911..61024
                     /gene="acrD"
                     /locus_tag="ACHLCY_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP0368433.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(61123..61182)
                     /gene="ypfM"
                     /locus_tag="ACHLCY_001127"
     CDS             complement(61123..61182)
                     /gene="ypfM"
                     /locus_tag="ACHLCY_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP0368434.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14435 a        16914 c        15632 g        14432 t
ORIGIN      
        1 tgcgatttgc gaaattgcct gatgcgcttc acttagcaga cgactatttc cggcaattcc
       61 tgtctcctca cctactgtgt caatgcagcc aacagcttaa ccatcgcggg cgtcacctgc
      121 tgtgtttcat aaacaatata taaatctgca gggatgcgct gtttgagcgg acggaaaatg
      181 acacctggcc agttcatttg tgcgtagctg tccgctatca atgtgatacc aatacccata
      241 ctgaccatgg cgagtaccgt ttgcggttca ttaacttcgc gaataacaac cggtgaaaat
      301 cccacctgct ggcaaactcg ctgcaaaaaa tcccagtcag tgtacacggg cggcattgta
      361 acaaaatact cgtcacgtag cgcttccagc ggaacggtgg aaaatgatga gagatgatgc
      421 tcttcaggca tcgccaccag aaacgccgat tcatgcaacc gtaagctggt aaaacctgtc
      481 ggtggttctg tcgccattcg ccagatcccg gcatcgagtt cacgtcgttc cagcagggcc
      541 atttgcatcg cgggcatctt ttcgcgaaaa agaacttcaa cgttaggatt ttccctgagg
      601 aatcgccgca taaccgggcg catccgtccc cacatagccg ttcccactac gccgagttca
      661 atccgccctg cttctccccg acctatttgt tcaacccgag ccaatacatt attagcattc
      721 accagcaatc gacgcgattc ttccatcaag attttgcccg cgtgtgtcag tacgacgctg
      781 cgcgaatggc gaataaaaag ctgcgtgccg agttgatttt ccagctcttt aatatgaata
      841 ctgagcggag gctgagacat atttaaacgc gctgctgcgc ggccaaaatg caactcttcc
      901 gctacggcaa gaaaataacg gagcaactta agatctgttc tgtatacgcg ttccataatt
      961 aagtgctcca tataataaat acatcgtatt aaattatcat ggcataaagt atttcaccac
     1021 cagatatccg acatacataa acacaatgga taatatacct ggtaatacat ttggtacagg
     1081 aataggcaag cgcacgattg taaacaaagc accaatgcca aaaccaacgc cggaagaaaa
     1141 taaaatatct ttcactaatt aacctttatc ataaaagcag ctctgaagag cagagctgcg
     1201 aatcctttta atgagccacc gctcgatgct ttatcttttc agggtcatga ttatatttaa
     1261 acccaaagaa aaatatcact gcgagaaaaa gagcatatcc tgcaaacacc agccagatag
     1321 tttgccagtc ttttacgcca tccaccgaaa agtaatctac tgccatgcca ctcagaatcg
     1381 agccaaccca tgcgccgaca ccatttacca tggtcataaa gagcccctgc gcgctggcac
     1441 gaatgctgga atcaacttcc tgttcgacaa ataccgaacc agaaatattg aagaaatcga
     1501 atgcacagcc ataaacaatc atcgacagca gcagcaaaat aaatccggtt gttgacggat
     1561 cgccataggc gaagaagcca aagcgcagcg tccaggccac catactcatc agcatgacgg
     1621 ttttaatgcc aaatcgcttt aaaaagaatg ggatagtcag tataaagccc acttctgcca
     1681 tctgtgaaac tgacagtaaa atggagggat atttcaccac aaaactgtca gcaaactccg
     1741 ggttacgggc gaaatcatgt aggaacggat taccaaaaac gttggtaatt tgcagtaccg
     1801 cacccagcat catggcaaag aggaaaaaga tggccatgcg tggattttta aacagcacga
     1861 aggcatccag acccagcttg ctggcaagcg atgtggtcgc ttttttctcc gcaaccggaa
     1921 tcttcggcaa agtcagcgca taagccgaca gcagcaatga cgcaccggac gcgatataca
     1981 gctgcagact actcaattcc agatgcagca ggcttactgc ccacatcgcg acaatgaacc
     2041 ccaccgtacc aaaaacgcga atgggcggga aagcggtcac cgggtcaagc cctgcctggg
     2101 caagacagga ataagagacg ctgttcgata acgcaatagt cggcataaac gccatcgcat
     2161 tgactaacat cacccaaaac atcatatccg gatcagttac ggatgccgca taaaaaagta
     2221 cgcccgcaca caccaggtga cacagcatgt atgcacgttc tgcgcgcagc catttgtctg
     2281 cgatgatccc cattatacca ggcataataa tcgcggcgat ccctttggaa ctgtaaacca
     2341 tgccaacatt agcgccggtg aaatgaaggg tattaatcat gtaagagccg agggtaacca
     2401 gccagctccc ccagataaaa tattgcaaaa aggacattac ctttaagcgc atcgcgatgc
     2461 tcataatcgt ttccttgtcg catcatttgg cctcacccgc gaggccgttt cttattgttt
     2521 ttctcaggca attttgcgca gaaagccaca aataaggttg atgaagttct gcttagagag
     2581 ctccgcagcc gccagcgttt gcgcatgtga caatttcaca tcgcttaaac cttccgccat
     2641 attggtaatc gcagagaccg caacgacttt aaggtcgcaa tggcgagctg aaataacctc
     2701 aggcaccaca gacataccaa caacatcccc accaataatt tgcatcatgc gaatttccgc
     2761 cgcagtctcg aaattcggcc ccggatacga gacgaacacg ccctccgtca gagggaaacc
     2821 ctcttctttc gccacttttt gtaacagtgc gcggtattcc gcatcgtagg catttgccag
     2881 tgagaagaag cgctctccaa aacgatcatc gttaagaccc accatcggcg taccgggcat
     2941 ggtgttgata tgatctttca atgcgaccag gctgcctgcc cccacttccg gacgcagcga
     3001 gcctgccgca ttggtgcaga acagtagctc gcagcccagc agcttaaagg tacgaattgc
     3061 gtcagtcata atggtcattc cacgaccttc gtagaaatgt ccgcgacctt tcatacatac
     3121 caccggtacg ccctggagat ggcccagcac taactccccc gcatgaccat gtaccgtact
     3181 taccgggaag cccggcagct tttcgtagga aattgcgaca gcgttctcaa tctgatcggc
     3241 cagcgcgccg agcccggaac ctaaaataaa ggccactcgt ggcgtgaaat caggtttata
     3301 agtcttgata atatctatgc aaaacagcgg gttatgagaa aattgaacct gagacataca
     3361 tatccttttt ctgtagggcg gaatctaacg cctttttata gcgaggttgt cccggctctt
     3421 accaatacac ttttctcacg cattcaatag cgattcgata tcgatggatt tatatctttt
     3481 ctctattaat ccacggtaaa aactgtattg gcgatattaa ttcctttgca ttaacaatat
     3541 gtcgttttta gcaaaccact ttattaagca atctactaat aacgatcggc atagggaatt
     3601 atgctatgaa aaaacatctt ttaactctga cactttcctc tatattagcg atacctgttg
     3661 tatctcatgc agaatttaaa ggcggttttg cagatatcgg cgtgcattat ctggactgga
     3721 ccagccggac taccgaaaaa tcgtcgacca aatcacacaa agatgatttt ggttatcttg
     3781 aatttgaagg tggcgctaac tttagttggg gagaaatgta cgggttcttc gactgggaaa
     3841 acttctataa cggtcgccat aataaaccag gtagcgagca gcgttatacc tttaaaaata
     3901 ccaaccgtat ttacctgggt gacaccggat ttaatctcta cttgcatgcg tatggcacct
     3961 acggttctgc gaatcgcgtg aatttccacg acgatatgtt cctgtacggt atcggctaca
     4021 atttcaccgg cagcggttgg tggttcaaac cgttctttgc taagcgttat acagatcaaa
     4081 cctattacac tggcgacaac ggctatgtcg ccggctgggt tgcaggttac aactttatgc
     4141 tgggcagcga gaaattcact cttaccaact ggaacgagta cgagtttgac cgtgacgcta
     4201 cctatgcagc gggtaatggc ggtaaagaag ggctgaatgg cgcagttgca ctgtggtgga
     4261 atgcaacatc acacattact acggggattc agtatcgata tgcggatgac aaactgggcg
     4321 aagattttta ccaggatgcg ataatctact ccatcaaatt taacttctga ttgattactg
     4381 ccggggctcc cggcagtatg aattcactct atatctgata caacggatcc ccttcccgac
     4441 cagccattag cgcattgccc tggtctcgta ccacgtccca taacgcttca gcggcggtgg
     4501 aaagtgaacg gttcttcctg cgcaccagca tcaactggcg ttcaacaacc ggcgtaatgc
     4561 gctttaccac cagtgggcta ccttcaggca atggtagcgc cagtgcggga agaatactga
     4621 tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa
     4681 tattcgcctg aataccattg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg
     4741 cgtaatcctg caacaccaat ttcgctccct gtaatgcctg ccagggtaca taatcttcga
     4801 cggccaaagc gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac
     4861 attgcagatc gcccactggg cctggatcga tgacgatgcc aaagtcgaca tctccctggc
     4921 gaatactttc catcacccac tgctgcggac gatcgtgcag gacaaactga atatctggat
     4981 agcggcgatg gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg
     5041 cagcgacccg aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc
     5101 tgttcagttc atccagcagt cgctcaagac gcaaagccag ctgctgccct gcatctgtaa
     5161 gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagttcct
     5221 tcacactgtg actcactgcc gactggctca ggccgatacg ctctcctgca cgactaaaac
     5281 ttttctcctg cgctactgtg acgaaaacct ttaattgttt taaagaataa ttcatctgtt
     5341 ttcttcatga atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag
     5401 aaccatcgat catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta
     5461 accctgatca cggtggtgtt gctggcctct ttctttccgg ccagaggcga tttcgtcccc
     5521 ttctttgaaa atctgaccac cgcagcgatt gccctgctgt tctttatgca cggcgcgaag
     5581 ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc gactgcattt gtgggtaatt
     5641 tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt ttgcctggtg gaaaccggta
     5701 aatgtcgacc cgatgctcta ctccggtttt ctctacttgt gcattctccc ggctaccgtg
     5761 cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct
     5821 gcgtcagcat ccagcctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg
     5881 aatgttcacg gtgcaggggg cagccttgag caggtcggta aaattatgct gcaactgctg
     5941 ctgccgtttg tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat
     6001 aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacagcgttc
     6061 agcgaagccg tcgttaatgg tatctggcat aaagttggct ggggatcatt gctgtttatc
     6121 gtggtggtca gctgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc
     6181 cgactgggct tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt
     6241 ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg
     6301 gtgctgcccc taatgatttt ccatcagatc caattgatgg tctgtgcggt gctggcgcgt
     6361 cgatacaaac gccagaccga acagttacag gcacagcagg aaagcagcgc cgataaagct
     6421 taaagcggac gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc
     6481 gtgatttgca ttagctcacc gagcttccct gccgggaatt catctttgcg ggcaaaccac
     6541 agcagatact cctccggcag gtcgattaag cgacgccctt tatatttgcc aaacggcatt
     6601 atcgtattgg ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag
     6661 catttccgct tcgtcgatga cttcaatgcc cagttcctgc gctttcgcca gtttagaacc
     6721 tgccgcttct ccggcaatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc
     6781 gcccagttca accagtcgag ctttagcgtc atcacgcgac atctggctta agctgcccgt
     6841 aagcaccacg gttttaccag caaacgggct gtcaatctct tccgcgttga taacgatcgg
     6901 cgcaggccag tgaacacctt ccgccaacag ctcgctgatg acattgcggt tgctttcttc
     6961 ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca acatcaggca ccttttgcag
     7021 ctcttcaatc gaagcggctt ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc
     7081 tgcggtggcc tcgccgactt cacggatgcc aagtgcatag aggaagcgag caaaggtggt
     7141 ttctttcgct ttttccagcg cgttaaccac gttttgtgcc gattttggcc ccatacgctc
     7201 cagtccggtc agtttgcctg cggtgagttt gaacagatct gccggagtat ggacatattc
     7261 tttttcaacc agctgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg
     7321 ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg caaatcaggc caccggtaca
     7381 gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa ccacataccg gacaatgcgt
     7441 cgggaataca acctcacgag tatcttccgg gcgttcagaa agcacgacgt taaccacctg
     7501 cgggatcacg tcgccagcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg
     7561 ttcgatttca tccgcattgt gtaaggttgc gttactcacc agcacgccag caacatggac
     7621 aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc
     7681 acgcacaaag gtcatctgct cctgcgccgg gaatttaaac gctaccgccc agcgcggggc
     7741 acgcgcgaca aagccaagct gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc
     7801 gtcgatatca aagcccagcg tcgggcggtc ttcttccact ttgtgataga acgccagcac
     7861 ttcttccgcc gattcacaaa gcgttacccg atcgctgacc ggcaaccccc actttttaaa
     7921 ttgcagtaaa cggccaagat gagtatccgg cagctcgcca ccttccagaa caccaacgcc
     7981 atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga
     8041 accagctgcc gcattacgtg ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc
     8101 gttaatcttt tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc
     8161 cgggatattc tctccgtgca gcttcagcgg aatagcgcga atagtacgca cattagacgt
     8221 gatatcttcc ccggtggtgc catcgccacg ggtcgcggca ctgactaaaa cgccattttc
     8281 atacagaata ctgacggcaa ggccatccag cttcagctca cagcaccagg tgactttctc
     8341 gttgtttttc agacggtcct gcacacgttt gttgaaagca agaaagcttt cttcatcaaa
     8401 aacgttatcc agtgacagca ttggtacttc atggcgtatc tggctgaaag ccgccagcgg
     8461 cgcagcgcct acacgttggg taggcgaatc aggcgtaatc agttctggat gtttggtttc
     8521 cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc
     8581 catcacatga taaagatatt catgatggcg aagcgtcgtt cgcagttctg tcagttgttg
     8641 ttcgattgat tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa
     8701 gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt
     8761 cctggtactc gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcacgacac
     8821 cgcccacttc atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct
     8881 cgtcaccgta agacggtacc tgcataaaga tggtgacacc cggagtagtg aaatccttca
     8941 tttcaggatc aaaggttccc ggtttcacca tattcgccag gctgaataac gccgggccgc
     9001 tgccatccgg gctaagatga cgatggtaaa tattcatatc gccaaaaatg aagcccgctt
     9061 gttgaatgct gttaagaagc agttcaccgt ttagctcgct accgtgatgc gccgcgacgt
     9121 tcatgataat caccgcttct ttgcgcttcg gtttatccat aactggagcc ggttccgcta
     9181 caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt
     9241 gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca
     9301 acggctgttc aggctgcggc tgataggcag gctgctgcac cggctgcgct ggacgcggag
     9361 catgttgcgg cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag
     9421 acgcataagg cggttggtac tggtgttgcg gcgacggacg agcagcctca tgctcctgag
     9481 cgttagccgg ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt
     9541 cgacatcctc gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat
     9601 cgcggaacat agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga
     9661 ttatggcgat cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct
     9721 ctgttgttct aacaccttgc caccacggca aacatttact cactaagagt atttgccgat
     9781 tacctcaagt gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt
     9841 tttgctgttt tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc
     9901 agaattttcc tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat
     9961 ctgccccacg cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg
    10021 ggattcggcg tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat
    10081 tctggtggct ctttacacag ctcgatgtct ggatcccgac tctcatgagt tacgttccgg
    10141 actggctaca atggctgagt tatctgttgt ggcctctggc tgtcatctct gtgctgttag
    10201 tgtttggcta tttcttctcc acgattgcta actggattgc cgctccgttt aacggtttat
    10261 tggctgaaca actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg
    10321 gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gcctggtatc
    10381 tgccgcgcgc aattgtattg ctaattcttt acttaatccc tggtattggg caaaccgtcg
    10441 cgccggtact gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc
    10501 ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa
    10561 tcaccaatat gcagtttggt gctttaacca gcctgtttac gatgatcccg ctgcttaatc
    10621 tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg
    10681 ataaacacgc gatgtggcgg taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa
    10741 cagggggggc ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgtata
    10801 tagatatgct aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc
    10861 ccaatttcat acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact
    10921 atcggtcaca cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag
    10981 gtggaatctc gtaaccccag cttcagcgtt aagtgccgta tcggtgccaa catgatttgg
    11041 gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc gaccagcggt
    11101 aataccggga ttgcactggc ctatgtagct gccgctcgcg gttacaaact caccctgacc
    11161 atgccagaaa ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg
    11221 gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc
    11281 gccagcaatc cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa
    11341 attcacgaaa aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta
    11401 tttattgctg gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc
    11461 accaaaggca agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc
    11521 gcccaggcac tggctggtga agagattaaa cctggcccgc ataaaattca gggtattggc
    11581 gctggtttta tcccagctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc
    11641 aatgaagaag cgatttctac cgcgcgtcgt ctgatggaag aagaaggtat tcttgcaggt
    11701 atctcttctg gagcagctgt tgctgcggcg ttgaaactac aagaagatga aagctttacc
    11761 aacaagaata ttgtggttat tctaccgtca tcgggtgagc gttatttaag caccgcattg
    11821 tttgccgatc tcttcactga gaaagaactg caacagtaat gccagcttgt taaaaatgcg
    11881 taaaaaagca cctttttagg tgcttttttg tggcctgctt caaactttcg cccctcctgg
    11941 cattgattca gcctgtcgga actggtattt aaccagacta attattttga tgcgcgaaat
    12001 taatcgttac aggaaaagcc aaagctgaat cgattttatg atttggttca attcttcctt
    12061 tagcggcata atgtttaatg acgtacgaaa cgtcagcggt caacacccgc cagcaatgga
    12121 ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa
    12181 agtcgaaccg ccaggctaga ctttagttcc acaacactaa acctataagt tggggaaata
    12241 caatgttcca gcaagaagtt accattaccg ctccgaacgg tctgcacacc cgccctgctg
    12301 cccagtttgt aaaagaagct aagggcttca cttctgaaat tactgtgact tccaacggca
    12361 aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct gggcctgact caaggtaccg
    12421 ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc ggttgaacat ctggttaaac
    12481 tgatggcgga actcgagtaa tttcccgggt tcttttaaaa atcagtcaca agtaaggtag
    12541 ggttatgatt tcaggcattt tagcatcccc gggtatcgct ttcggtaaag ctctgcttct
    12601 gaaagaagac gaaattgtca ttgaccggaa aaaaatttct gccgaccagg ttgatcagga
    12661 agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac
    12721 gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc tttgaagggc atattatgct
    12781 gctcgaagat gaggagctgg agcaggaaat catagccctg attaaagata agcacatgac
    12841 agctgacgca gctgctcatg aagttatcga aggtcaggct tctgccctgg aagagctgga
    12901 tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg
    12961 caacatcctg ggcctgaaga ttatcgacct gagcgccatt caggatgaag tcattctggt
    13021 tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt
    13081 catcaccgac gcgggtggcc gtacttccca cacctctatc atggcgcgtt ctctggaact
    13141 gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg aaaaatgacg actatctgat
    13201 tctggatgcc gtaaataatc aggtttacgt caatccaacc aacgaagtta ttgataaaat
    13261 gcgcgctgtt caggagcaag tggcttctga aaaagcagag cttgctaaac tgaaagatct
    13321 gccagctatt acgctggacg gtcaccaggt agaagtgtgc gctaacattg gtacggttcg
    13381 tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt
    13441 cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa cagtttgctg cttacaaagc
    13501 agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt accatggaca tcggcggcga
    13561 caaagagctg ccatacatga acttcccgaa agaagagaac ccgttcctcg gctggcgcgc
    13621 tatccgtatc gcgatggatc gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg
    13681 tgcctcggct ttcggtaaat tgcgcattat gttcccgatg atcatctctg ttgaagaagt
    13741 gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc
    13801 gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca ccggctgccg caacaattgc
    13861 acgtcattta gccaaagaag ttgatttctt tagtatcggc accaatgatt taacgcagta
    13921 cactctggca gttgaccgtg gtaatgatat gatttcacac ctttaccaac caatgtcacc
    13981 gtccgtgctg aacttgatca agcaagttat tgatgcttct catgctgaag gcaaatggac
    14041 tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca cttctgttgc tggggatggg
    14101 tctggacgaa ttctctatga gcgccatttc tatcccgcgc attaagaaga ttatccgtaa
    14161 cacgaacttc gaagatgcga aggtgttagc agagcaggct cttgctcaac cgacaacgga
    14221 cgagttaatg acgctggtta acaagttcat tgaagaaaaa acaatctgct aatccacgag
    14281 atgcggccca atttactgct taggagaaga tcatgggttt gttcgataaa ctgaaatctc
    14341 tggtttccga cgacaagaag gataccggaa ctattgagat cattgctccg ctctctggcg
    14401 agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg
    14461 gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc tgtagacggt accattggta
    14521 aaatctttga aaccaatcac gcgttctcta tcgaatctga tagcggcgtt gaactgttcg
    14581 tccacttcgg tatcgacacc gttgaactga aaggcgaagg cttcaagcgt attgctgaag
    14641 aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt tgatctgccg ctactggaag
    14701 agaaagccaa gtctaccctg actccggttg ttatctccaa catggacgaa atcaaagaac
    14761 tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt
    14821 aattcttgcc gcagtgaaaa atggcgccca tcggcgccat tttttatgct tccgccagcg
    14881 gcggcaaaat caattcatcg ctctcatgct gctgggtgta gcgcatcact tccagtacgc
    14941 gcaaccccgc acggtgcacc gcatcgttta acgccttccc tttcagcaaa ccactgataa
    15001 gctgagcaca aaacaggtcg ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa
    15061 tgacattcac gctgtcggca ctgaccacca ccacctgcat ctcctgattt tcttcattac
    15121 cggaggcgct ggtaatcacc acccatttta atgtgtctga aagcagactt tttgcggcag
    15181 caatggcact gtcgagatcg cggcaatttt taccggtcag gatttccagc tcaaagatat
    15241 tgggggtaat tccctgcgcc agcggcagta aatattgtcg atacgcctcg ggaaggtcag
    15301 gtttgacata aattccgcta tcaatatcgc caatcaccgg atcgaccatg atcaataggt
    15361 caggatggtc tttgcgtagc gcagtcagcc actcggcaag gattttgatt tgcgatgccg
    15421 ttcccatata gcccgtggtt acagcgcgca gctggcgcag cgcatcacgc tcctgaagcg
    15481 cacgcaaata accgctaaac cattcatccg gaatcgcacc accgtagaaa gtgtcataat
    15541 gcggcgtatt gctcagcaat accgtcggca cggcaaaaac attcagaccg ttctgtttga
    15601 tagcaggcac ggcaatgctg ttgcccacgc tgccgtaaac cacctgcgac tgcacggcga
    15661 cgatatccgc ctgcagcgcc ctgctcttat cgttaaacaa caacaaacta ctcatttaat
    15721 tttttctcct tgccgatgat cctcatcgta ttccaaccga aactttacct gattctggca
    15781 gtcaaatcgg ctatcacaaa acaaggataa ggtaattcaa tgaagaaaat catttgtctg
    15841 gtcattacac tactaatgac actccccgct tacgcgaagt taactgccca tgaagaagcc
    15901 cgcatcaacg ccatgctgga gggattagca cagaaaaagg atttgatatt tgtgcgcaac
    15961 ggtgatgaac atacctgcga tgaagcggtt tctcatctgc gtctgaagct gggcaatacc
    16021 cgtaaccgca ttgacactgc cgagcagttt attgataagg ttgcttcgtc gtcatcgatt
    16081 accgggaagc cgtatattgt gaagatcccc ggtaagagcg atgagaacgc acagcctttt
    16141 ttacatgcgc taattgcgca gacggataaa acggtgcctg cgcaataatc ttaaatcccc
    16201 gccccctggc taaaatgctc ttccccaaac accccggtgg aaaggtagcg atcgccacga
    16261 tcgcagatga tcgctaccac caccgcacca gggttagctt ttgccacccg cagtgctccg
    16321 gcaaccgcgc cgccggagct gacgccacag aatattcctt cccgcactgc cagttcgcgc
    16381 atggtgtttt ctgcatcgcg ctgatgaata tccagcacct catccaccag agaagcgttg
    16441 aaaatccccg gcagatattc cgcaggccag cggcgaatgc cgggaatact gctgccttct
    16501 tccggctgca ggccaacaat cgttactggt ttggattgtt cgcgcataaa gcgtgaaacg
    16561 ccggtgatag tgccggttgt tcccatgctg gagacaaaat gggtaatgcg cccgccggtt
    16621 tgctgccaga tttccggccc ggtagtggtg taatgggcgt aagggttatc gggattatta
    16681 aactgatcga gcagctttcc ttcgccacga ttcgccatct ccagcgccag atcgcgcgca
    16741 ccttccatgc cctgctcttt ggtgacaaga atcagttccg caccataagc acgcatcgcc
    16801 gcacggcgtt cctggctcat gttgtcgggc atcagcaatt tcatgcgata gcctttcagc
    16861 gcggcaatca ttgccagcgc aatgccggtg ttaccactgg tggcttcgat taagacatca
    16921 cccggtttaa tttccccgcg cttttccgcc tcgacgatca tcgaaagtgc cgcacgatct
    16981 ttcaccgaac ctgccgggtt attgccttcc agttttaacc acacttcact gccgttatcc
    17041 ggccccattc gctgcaactt caccagaggc gtattgccta ttgtttgttc taatgtactc
    17101 acgatctcta tccatacgtg gtgttgcctg atgcgacgct tgcgcgtctt atcaggtcta
    17161 caggttacaa accttgccat taaaaagccc ggatcgcggg aagcgcctcc gggcgtttaa
    17221 cattcactca acctatcagg cgctttgtgc gagagcaagt tcctcatctc gggttttgat
    17281 acgctcgtcg ccgttataca gccgcgcatg ttgcagacca acgaataaac gctcgccacg
    17341 ctgcggggca tcgtcgccat gcatcacgac cgtcagcggt tcgttgtacc accccagcgg
    17401 ctgcaccact aattgggtgt agtgaccttt cgggctggct tccagtacct gtaccggcag
    17461 cggcgaatcg agactggtac ggcggctgat atccacttcc cacgggcgca ggaagagatc
    17521 caccggaccc tgatacgcag gtgtgtagcc cagcggccag cgatgcgcgc caacatggaa
    17581 ctgcccgccg cgaatggttc cctgcaggcg gttcacttcg cccataaatt cgagtacaaa
    17641 acgggtcgct ggttcgcgcc atacctgatt cggcgcatca gcctgttcaa tattcccctg
    17701 actcatcacc actacacgat cggctacttc ggtcgcctct tcctggtcgt gggtcacgaa
    17761 cacgctggtg aatttcagct cttcatgcag ttgacgcagc cagcgacgca gctctttacg
    17821 cacctgcgca tccagcgcgc caaacggttc atcaagcagc agaatttgcg gttctacagc
    17881 cagcgcgcgc gccagcgcca cgcgctgttt ctggccgccg gaaagctgtg ccggataacg
    17941 atctgccaga tgagcaagct gcaccatctc cagcaatttt gtcactttcg ctttgatggc
    18001 tgcggcattc gggcgctcgc gacgtggcag caccgtcagg ccaaaagcga tattgtcgaa
    18061 caccgtcata tgacggaaca gcgcgtaatg ctggaacacg aaaccgacct tgcgatcgcg
    18121 cgcgtgcagg cggctgacgt cggtgccgtg gaagcgaata tgcccgctgg tttgatgctc
    18181 cagcccggcg ataatgcgca gcagcgtggt tttcccggaa ccggacggcc ccagcaacgc
    18241 gaccatctga cctgaaggaa tatccagtga gatatcgttc agcacctggg tgcgaccaaa
    18301 cgacttctta atattggcaa tctcaatgct catgatgttc ctcctgctgt gcgcgtttat
    18361 cctgattctc caggcgccac tgcaacatac tttttaaaaa cagggtgata atcgccatca
    18421 gcgttaacag tgccgcagcg gtaaaggagc cgacggtgtt gtagtcctgc tccagcaatt
    18481 caatctgtaa cggcagcgac agggtttcgc cgcgaatcga gccggaaacc accgacaccg
    18541 cgccaaactc gccaattgcg cgggcgttgg tcaacaccac gccataaagc agcgcccagc
    18601 gaatgttcgg caatgtgacg cggcgaaaca tctgccagcc agacgcgcca agcaaaatcg
    18661 ccgcttcgtc ttcctggctg ccctggctta acatcaccgg caccagctcg cgcaccacaa
    18721 acggacacgt cacaaagatg gtgaccagca ccattccggg ccaggagaac ataatttgca
    18781 gattatgttc atccagccag ccgcccagcg ggccgttaga gccgtagaac agcagataca
    18841 ccagaccggc aaccaccggc gatacggcaa acggaatgtc cagtagcgtc agcagtaact
    18901 ggcgtccagg gaagttaaag cgcgtcacca gccaggccag cagaatgccg aacaccaggt
    18961 ttaccggtac ggcaatcagc gcgatcatca ccgtcagcca gatggcgtgc agcatgtccg
    19021 gatcggccag attctgtaaa accggcatca gccccttgct gaatgcctgc acgaagatgt
    19081 aaatcatcgg caccagcagg atgaacgctg aaaccagcat cccgatgcca attagaaacc
    19141 atttgcccca gttaatcggg cgcgcgtcat aacgcttcaa ttgggtaact tccgccatta
    19201 atgacctacc acacgccgac caaagcgact ttgcagagtg ttaattgaga acagcagcag
    19261 cagggatgcc gcgaggatca ccgaagcaat cgcgctcgcc gccgggtaat caaactcctg
    19321 caagcgcaca aaaatcatca gcgacgtcac ttccgtcttc caggcgatgt tcccggcgat
    19381 aaatatcacc gcgccaaact caccaagact acgggtaaac gacaacgcca cgcccgccac
    19441 cagcgctgga gaaagttccg gcagcaccac tttgcagaaa ctctgccagc gcgttgcacc
    19501 aagcgtttcc gccgcttctt catattccgg gcctaactct tccagcaccg gctgcacggt
    19561 acgcaccaca aacggaatgc tggtaaaggc catcgccacc gcaataccaa gccaggtata
    19621 ggtgactttg atatcaaact tcgccagcca ttcaccgtaa aaaccgttta cggaaaagag
    19681 cgaggccagc gttaaaccgg cgactgccgt tggcagcgca aagggtaaat ccatcagcgc
    19741 atcaagcagc gtgcggcccg gaaagcgata acgtgttaaa atccacgcca tcagcagacc
    19801 gaaaacgccg ttaaaaatcg atgccacaaa cgccgacagc agcgtcactt tgtaggccgc
    19861 gaccacttgt gggttggtga tcacctccca gtactgcgcc cagctcatct gagccagctg
    19921 catcaccagc gcggagagcg gcagcagcaa aatcaggcac acaaacagca gactggtgcc
    19981 gaggcttaag gtaaagcccg gcagcacgcg tcttgatgaa actgcaaaca tcagttacgc
    20041 cccgccgcta acagcttgtc taactcgccg ccgctggtga agtgggtttt catcacttcc
    20101 ggccaggagc caaatttgtc ttccacgcgg aacagctcgg tctgcgggaa tttatctttc
    20161 agtttgtcca tgacttccgg gttatttacg cggtagtaat agtcggtgat gatggtctgc
    20221 gcctgcgggc tgtagagcca gttcaggtag gcttttgctg ctttttccgt accgttggcc
    20281 tgcacgtttt tatcgaccca cgccaccggg aattccgcca gaatattggt tttcgggatc
    20341 accacttcaa agccctgtgc ttcatactgt ttacggatgt tgttcacttc cgattcaaag
    20401 ctaatcagca catcgcccag gccacgctcg gcaaaagtgg tggttgcgcc acgaccgccg
    20461 gtatcgaaca cttcaacatt tttcaggaac tgggtcataa actgttcggt tttggcttta
    20521 tcgccaccgt cagctttatc cgctgcgccc catgccgcca gataggtata acgcgcgtta
    20581 cccgacgttt tcgggttcgg gaaaatcagc ttcacgtcgg agcgcaccag gtcgttccag
    20641 tcgtggatat tcttcgggtt gcccttacgc accaggaagc ccatggtgga gtagaacggc
    20701 gagctattat tcggcaggcg cgactgccag tccgccggga tcagcttgcc tttgtcatgc
    20761 aggatttgta cgtcagtcac ctggttataa gtgacaacgt cggcttttaa gccctgtaaa
    20821 atcgccagcg cctgttttga tgacccggca tgagattgtt taatcgtcag tttgtcgccg
    20881 ccgttatctt tggcccattg ctgttcaaac ggcgggttca gggcggcaaa cagctcgcgg
    20941 gagacgtcat aagaactgtt cagcagttcc gttgcctgta catggcctgc cagcagcaga
    21001 gaagcgacca gcgcgagtga gttctttttc agtaagttaa cggccattgc gcacccttat
    21061 atatttaatg actttctaat agccatcata tttataacgg acgtgaaagg agtaacggtt
    21121 ttatataccg tttggtgatt tggaagttga aaagggaata agaaccgggt ttaaaacgaa
    21181 atcctcccca cacatgcagg gaggaaacag aggtgaatca gataccgacg ctaaccgtct
    21241 ccggcagtgt gctgccgcca tcaatcacat tctgtgtacc ggttaaatag ctggattcat
    21301 ccgatgcgag gaaggccgcc agttcgccga cttccagcgg atcggcgagg cgacgcagcg
    21361 ggattgcttt tgccatatca gtcagcaccg actctggatc ttccgggttc gactggcggg
    21421 caatgctttc cgccattggc gtgcggacgt accccgggca gatggcgtta acgcgaatac
    21481 cggattgcgc gtactccacc gccagcgatt ttgtcaggcc aacaatcgcc gctttcgtta
    21541 aggcgtacgc cgtttcaccc ggatcggcca ccatatcacc agtgactgaa gacatcatca
    21601 caatgcgacc atctttgcgc gcaatcatct ctggcagcac cgccttcgtg acgttccata
    21661 cgcctttaat attgatgtca atatggaaat cgcgatcttc atcgctcata tcgaggaaac
    21721 tgcccagacg acaaacgcct gcgttattca ccaggatatc aatgcgccct tctttttcct
    21781 tcgcgcgttt gatagctgcg gctaccgacg ccgggtcacg cacatcggcg acaaccgccg
    21841 tacagcgatg accacgacca cacagttcgt ccgccagctt ttcgatctca ggggagatat
    21901 ccagcaagat taggtttgcg ccatgacgcg caaaagttct ggcaattcct tcgccaattc
    21961 cctgcaatgc gcccgtaatc agtgctgtct tgcccgtgag tttacccatt ttaatgctct
    22021 ccttgttaat acagagcgtt acactccatt ccctctgaaa atacaggata aacaaaatat
    22081 tgctgcggat cagctcacta attcaggaaa atctcaatcc agtcgctgct ttatgcatca
    22141 tttcaggcgt tgtgttagct cactgctgcg ctgaatcatt ttcaatgact cgacatcatt
    22201 gagttgcaca agaccaacaa acagtaaatc ggtaacagaa ttttgcgccg tgcgggtaga
    22261 catcgacgag ctacgccatt cggtttcacc ggaaacggta tcgagcgtga aatgcgccag
    22321 ccgccgcagc ggagagtcag ccagagaagt aatggcaatc accgttgccc cctgctttcg
    22381 tgccgcttct gcgcacaaga caatctcttt tttactgccg ctgtaagaga tagcgatttg
    22441 tacatcgcct tttttcaacg cctgggaaac ggtggcttgt acgtgcgtgt cggcttcgca
    22501 ggccacgcga tatccaattt tcatcagttt gaacgacaga tcgcgcccca ccagcgccga
    22561 tccgcccagg cccgttatct gaataaattg cgctttactg atgacatcaa tgattttttg
    22621 cagccgcgcg tagtcgaaca acgcgcaggt ttgctccagc gccagctctt tttcgcgatt
    22681 cagtttgcgg gcaatcactt ccagcgaatc atcactggta atcgaactgt gcaggtgcag
    22741 cgccgtggca ttcgttttct cccggctggc actgtattcg ccaattaacg ccatacgcaa
    22801 ttcagtaaat ccttgtgcgc cgagcttctg ggcaaacttc acaatactcg actggctgat
    22861 gccgagctgt ttcgccatct tgcgcgaaga gacggattgc agctcactca cgttggcctg
    22921 cagaaagtca gcgatttttt gctcgttttc ggtgaattca ctccccgcat tgcttatttt
    22981 cgtcaggtac aacatcttcc actctcctgg gctggcagca taaattccgc ttaagttctt
    23041 atgacgctct tcacactctg cgagtgtaga ctcaatgatt cctttaagtc tgtcaattcg
    23101 ggatatatga ttccatacat ctgcaattat ttgatcgtaa atagtaaggt caccaccgat
    23161 gcaactagaa aagatgatta ctgaaggctc gaacaccgcc tcggctgaaa ttgaccgcgt
    23221 atcgacgctg gaaatgtgcc ggattatcaa cgatgaagat aaaaccgtac cgcttgccgt
    23281 tgagcgcgtc ctgccggata tcgccgcggc gatcgatgtt atccacgccc aggtcagcgg
    23341 cggcggtcgt ctgatttatc tcggtgcggg aacatccggt cgtctgggga ttctggatgc
    23401 cagcgaatgt ccgcccacct acggcgtgaa actgggtctg gtggttggtt tgattgctgg
    23461 aggcgaatat gccattcagc acgcggtgga aggcgctgaa gatagccggg aaggtggtgt
    23521 taacgatctg aaaaatatta atttaacggc acaggatgtg gtggttggca ttgctgccag
    23581 cggtcgcacg ccgtatgtga ttgccggact ggaatacgca cgccagctcg gctgccgcac
    23641 agtgggaatt tcctgtaatc cggggagtgc cgtttccacc accgccgagt ttgccattac
    23701 accgattgta ggtgccgaag tggtcactgg ttcttcgcga atgaaagcag gcacagcgca
    23761 gaaactggtg ctcaatatgc tttccaccgg gctgatgatt aaatctggca aagtgttcgg
    23821 caacctgatg gtcgatgtgg tcgccaccaa cgaaaaactg catgtgcgcc aggtcaatat
    23881 cgttaaaaac gccaccggat gtaatgccga acaagcggaa gcggcgttaa ttgcctgcga
    23941 gcgcaactgt aaaacggcca ttgtgatggt gctgaaaaat ctcgatgccg cagaagctaa
    24001 aaaacgcctg gatcaacacg gcggctttat tcgtcaggtt ttagacaagg aataacccat
    24061 ggccaaagag atcagcagtg aacttctgaa caccattctt acccgtgtcg gcggaccggg
    24121 aaatatcgcc agttgtggta actgtatgac gcgcctgcgt ctgggtgtac atgacagttc
    24181 actggttgat cccaatatca aaacgctgga aggcgtgaag ggcgtcattt tgaccagcga
    24241 ccaggtgcag gtcgtttttg ggcctggtaa agcgcatcgt gccgcaaaag cgatgagcga
    24301 gctgctgggc gaggcaccgg tacaggatgc cgcagaaatc gccgcccaga acaaacgtca
    24361 gttaaaagcc aaacaaacct ccggcgtgca acaatttctc gccaaattcg ccaccatctt
    24421 cacgccgctg atccccggtt ttattgccgc cggtctgctg ctggggatag cgacgttaat
    24481 tgccacggtg atgcacgttc cggcagatgc tcagggcacg ctacccgacg cgctgaattt
    24541 tatgaaggtg ttcagcaaag gtttgttcac tttcctggtg attctggtgg gctataacgc
    24601 cgcccaggca tttggcggca cgggcgtcaa tggcgcaatt attgccgcac tctttttgct
    24661 cggttataac cccgctgcaa ccaccggtta ctacgccggt tttcacgatt tctttggtct
    24721 gcccatcgat ccgcgcggca atattatcgg cgtgctgatt gccgcgtggg cctgcgcccg
    24781 cattgaaggc atggtgcgcc gctttatgcc ggacgatctc gacatgctgc tgacctcgtt
    24841 aatcaccctg ctgatcaccg ctacgcttgc gtacctgatc attatgccgc tgggtggctg
    24901 gttattcgaa ggtatgtcat ggctgtttat gcacctgaac agtaatccgt tcggttgtgc
    24961 ggttttagcc gggctgttcc tgatcgccgt ggtatttggc gtgcatcagg gctttattcc
    25021 tgtttacctc gcgttaatgg acagccaggg attcaacagc ttatttccca tcctttcaat
    25081 ggcaggcgcg ggccaggtgg gcgcggcgct ggcactctac tggcgggcgc aaccgcacag
    25141 tgcattacgc agtcaggtac gcggggcgat tattcccggt ctgctgggcg ttggtgaacc
    25201 gttgatttac ggcgtcaccc tgccccgcat gaaaccgttt attaccgcct gtttaggcgg
    25261 cgcagcgggc ggtttgttta tcggcctgat agcctggtgg ggtctgccga tgggcttaaa
    25321 cagcgccttt gggccgtctg gtctggtggc gctgccgctg atgacttccg cacaaggcat
    25381 ccttccggca atggcggttt atgctggcgg gattctggtg gcatgggttt gcgggtttat
    25441 tttcaccacg ctctttggct gccgtaacgt caatctggac tgatatcatg aaacggacaa
    25501 tgctctatct ttctctgctg gctgttagtt gtagcgtcag cgccgcaaaa taccctgttc
    25561 tgacagaaag ttcgccagag aaagcagggt tcaacgtcga acggcttaac cagatggatc
    25621 gctggattag ccagcaagtg gacgccggtt atcccggcgt aaacctgctg atcattaaag
    25681 ataatcagat tgtgtatcgc aaagcctggg gagcggcgaa aaagtacgat ggcagcgtgt
    25741 tgatggaaca gcctgtcaaa gccaccaccg ggacgctgta tgacctggcc tcaaacacca
    25801 aaatgtacgc cacgaacttc gccctgcaaa agctgatgag cgaaggcaaa ctgcatcctg
    25861 acgatcggat tgcgaaatat attccggggt ttgccgatag tccaaatgac accatcaaag
    25921 gcaaaaacac gctgcggatt tctgacctgc tgcatcacag cggcggtttc ccggcggacc
    25981 cacaatatcc gaataaagcg gtcgcgggcg cgttatattc ccaggataaa ggccagacgc
    26041 tggagatgat caagcgcacg ccgctggagt atcagcccgg cagcaagcat atttacagtg
    26101 atgtcgatta tatgctgctt ggatttatcg tcgagtcggt caccggtcag ccgcttgacc
    26161 gctatgttga agagtcgatt tatcgcccgc tcggcctgac acatacggta tttaacccgc
    26221 tgctgaaagg ctttaaaccg cagcaaattg ccgccacgga attgaacggc aatacccgcg
    26281 atggcgtgat ccatttcccg aatatccgca cctccactct ctggggtcag gtgcacgatg
    26341 aaaaagcctt ttattcgatg ggcggcgttt ccgggcacgc aggtttgttt tccaataccg
    26401 gcgatattgc ggtgttaatg caaacgatgc tgaacggcgg gggctatggc gatgtgcagc
    26461 tgttcagtgc ggaaacggtg aagatgttca ccaccagctc taaggaagat gccacttttg
    26521 gcctcggctg gcgcgtgaat ggtaatgcca ccatgacgcc gacgtttggc acgctggcaa
    26581 gcccgcaaac ctacgatcac actggctgga ccggaacggt gaccgttatc gatccggtga
    26641 atcatatggc gattgtgatg ttaagcaaca agccacattc gccggttgcc gatccgcaaa
    26701 agaatcccaa tatgttcgaa agcggtcagc tgccgattgc aacgtatggt tgggtagttg
    26761 atcaggtgta tgcggcgtta aagcaaaagt aataaaaagg ccagtcagta atgttactgc
    26821 ctggccttta atcaatgaat cagaaacgct tacagcgcca tcaacttgtc cagcgacggt
    26881 gcgaaataat agccgccggt taccggtttg gtgaaacgca acatcgcatc acgcttacca
    26941 tcggtatcgc caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag
    27001 gcgcagaagt acagaccgtg aatgccactg gcagtgccgt acggcaggct ctggcgaaca
    27061 atcttcagcc ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga ggtttccgga
    27121 cgttcgtcgc cgtcgatctc ttcgttggcc tctttggtgc gcccgatcat catctcctga
    27181 tcgtgaacgc tcatccggtt gagctgcttc aggttgtgtt cccaacgctg gacaaacaca
    27241 tagctgccgc ccgcatccac gccgtctttg ataaccgcca cttcgcgacg cgtctcttca
    27301 cccgccgggt tttccgtacc gtcaacaaag ccgctcaggt cacgctcttc aacccaacgg
    27361 aagccgtgga tctcttcttt cacttcaatg cagtcaccaa aggcttccat cgccgcctgg
    27421 gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg
    27481 gtggtcggcg caaggccttt accgtagccc ggaaaatctt tcagctcttc tgccccaacg
    27541 ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc aagatgcgcg
    27601 tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa atgttttact ggccgcacgc
    27661 agggcgtcaa cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct
    27721 ggcaaaatgc cactctgaac ctgagacatt gttcctcctg aaaataataa tgccacgcaa
    27781 tccgtggcat tgttgagcgc tattttacgt gttttttcgc ggcggcggtt tgttcagacg
    27841 caaattaacg ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag
    27901 gcattaaccc ttcaggacca ctccattccc cgctgaactg gtagctaata tgttgactgc
    27961 cttcgacttt acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa
    28021 atgctttgct gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa
    28081 tatcgctatc cagcacgtca atgcgactga tcgtgccttg atcgccgtta atcaccatcg
    28141 ccacgttgtc gcccttcatc acttcaaaaa agcgcaccac gttgccgttc gcggttttca
    28201 ttccgctgcg caggcgataa tcgccatcaa gcgcatcggc aatggcttgt tcttgcagtg
    28261 gtgtggacgc cgttaattca ccaacgccct gctcgctcac tttggtggac gacccaaacc
    28321 agttccacgg gttagcggcc gaccagttaa ctgacgacat cgtggaacag ccggtcagca
    28381 tcaacggcat agcgcataac attaaacgca agcgatttca tgtcacttcc tttggttatt
    28441 caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta
    28501 aaacaagcgc gtaaacgtcg attggtcagc agccagatta acgccacaat atccgccact
    28561 accagcgcca gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccag
    28621 caaagtaaaa ccacctgcgc caacagcaac agaaaataga gcacatgcca agtgcgagga
    28681 aatgtagccc gccgaccgct caacaaaaac gccagcaccg ccggaatgcc tggaatcagc
    28741 cccagccaga aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctcg
    28801 cgcgacgcac cggcaatgac aaacagcacc caagttcgcg cctgaagcaa tagcacaagc
    28861 cagaagagca agggtaaacg caggcgtccg tgcgcatcat aatggacagg atggaactca
    28921 gtactcttca tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc
    28981 caggcgttca tacattccga gcaccatgtc gttatcttcc ggcacattga tctgaatttt
    29041 cgggcagcca cgagcaatca gctttttctc cagccgatta agcaacgcat tggcaatccc
    29101 acgcccacga aactctggat gcacgccaag ataataagca gacccgcgat gcccgtcata
    29161 accgcccatc accgttccga ccacttcact gtttacctca gcgaccaaaa acaaactgac
    29221 gtcatggttc atcttacgct cgatgtccat ttccggatcg ttccacggac gcagcaagtc
    29281 gcaacgctcc caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc
    29341 catggtattc gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt
    29401 agccaatatc tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg
    29461 aaatgaaaag taaaacaatt ctcaacatca aaccgtcgta acggattacg cgatacgata
    29521 taacatctgg aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa
    29581 acactcactt cgcgccgcca ggtgctgaaa gccggtttgg ctgccctgac gttgtcagga
    29641 atgtcgcaag ccatcgccaa agacgaacct ttaaaaacca gcaacggaca cagcaagccg
    29701 aaagccaaaa aatctggcgg caaacgtgtc gttgttctcg atccaggcca cggcggaatt
    29761 gataccggag cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcgattgct
    29821 aaaaacgtcc gttccatttt gcgtaatcat gggattgatg cgcgtttaac gcgttctggc
    29881 gatacgttta tcccacttta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg
    29941 tttatgtcaa ttcatgccga tggctttacc aacccgaaag ctgccggtgc ttcggtattt
    30001 gccctctcta accgtggggc aagtagcgca atggcgaaat acctgtctga acgcgaaaac
    30061 cgcgccgatg aagttgccgg taaaaaggcg actgacaagg atcacctatt gcagcaagtg
    30121 ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc tgacgctcgg ctcgcatatt
    30181 ctgaaaaaga ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt
    30241 gtggtgttga aatcgccgtc ggttccttcg gtgctggtgg aaacctcgtt tatcaccaac
    30301 ccggaagaag agcggctgtt aggcacggcg gcgtttcgtc agaaaatcgc cacagcgatt
    30361 gctgaaggcg tgatcagtta tttccactgg ttcgacaacc agaaagcaca ttcgaaaaag
    30421 cgataagtta tgaaacccga cgcacaccag gttaaacagt ttctgctcaa ccttcaggat
    30481 acgatttgtc agcagctgac cgccgtcgat ggcgcagaat ttgtcgaaga tagttggcag
    30541 cgcgaagctg gcggcggcgg gcgtagtcgg gtgttgcgta atggtggtgt tttcgaacag
    30601 gcaggcgtca acttttctca cgtccacggc gaggcgatgc cagcttccgc caccgctcat
    30661 cgcccggaac ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg
    30721 cataacccgt atgttcccac cagccacgcg aatgtgcgtt tttttattgc cgaaaaaccg
    30781 ggtgcagagc ccgtctggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt
    30841 gaagaagacg ccattcactg gcaccgcacc gcccgtgacc tgtgcctgcc atttggtgaa
    30901 gacgtttatc cccgttacaa aaagtggtgc gacgattact tctacctcaa acatcgcaac
    30961 gaacagcgcg gtattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac
    31021 tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt accaattgta
    31081 gagcgacgta aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc
    31141 ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca ctctgtttgg cctgcaaact
    31201 ggcgggcgca ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat
    31261 tatcagccaa aagatggcag cccagaagcg gcgttaagtg agtttattaa ggtcagggat
    31321 tgggtgtaac tccctcaccc ccattcccgc atccgctgat gcaacgtcaa cgacggcttc
    31381 tcggcaaaca gctgctggta atccgtggca aattgcccca gatgccagaa tccccactgc
    31441 atggcggcgt cttttaccgt tgtgctttgc gaccacgggc ttatcagttc gcggcgtacg
    31501 gcgttcaggc gaatgcgttt cagccacgcg tttgggccaa tgcctaaaat agcgtgaaac
    31561 gcgttttgta gcgtgcggcg actgacatgc agttgattac acaagtccag caccgtcacc
    31621 ggctcagaca tattttccag cacatattca cgggcgcggg aaagtaatct acggtaactc
    31681 tgatgactga tgctttccgc cgtcaccatt ggttgcgcgt cttccagcat cgcccccatc
    31741 gccattagca aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg
    31801 ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc acagcgcggc tttatgctgc
    31861 tctttcactt ccagcgccga ctggctacgc agcatatgta atacccgatc cgggttatgc
    31921 aaaaagttag cctgccgggt gatgacatct tcagaaagca ccacgcccag gatcgtgtaa
    31981 tcatccggcg tgctcagttc aaattcagtg ccaccagggc gggtggcgat ttccgcgctt
    32041 cccagacatt gcgaaccgat aaatccctgc tcaccgcgcg tcgccggaat gccaaaccag
    32101 aacgagttcg gccagaccag gcacgactga cgcagcgcca gaccggtgta ttcacgaaaa
    32161 acctgaatat catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc
    32221 tgatcgtaaa tctgctgcca ggcggtaatc gttaaagcat gttcatagac atccgttgtc
    32281 tgtcgttgat gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag
    32341 ggttcatgat aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg
    32401 ggtgttgtag gacacccggc acctccgaca ggttaatggg gcttgagacg ataacgacta
    32461 ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc
    32521 agcggccagc caaagtgggc agccacttct cctgccgtca ttccctgacg tactgatgtt
    32581 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccctt
    32641 acgggttgct tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    32701 cgaccgatct ccttgcggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    32761 tccagcgccg cacgacacgc cgccagatcg ccttccacca ccagcgttaa gcgaccaggg
    32821 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atccgctgca
    32881 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    32941 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    33001 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    33061 tcagacggtg gcgggacata ggtcaccagc tgtacgtcgg cagatttcaa cgctgcatca
    33121 atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    33181 atcccggcgg ttgatgagag ataagaaccg gtacgcgaaa ctacatgtgc caggaaggcg
    33241 gtgttttctg cgtcattggc ccactggaaa gctgcgccat tttcaatatg tgcaaccatc
    33301 gcatccagac cggcacgcac ttccgccggg tttggcccac caagcataat cagcacttca
    33361 ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca gcgagcgacc atataccact
    33421 tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt aggtgacgtc gtcagaatcg
    33481 gcagaaatca gcccgaggct acgaatatgc ggcggcaatt ttagttcacg cgcaaattca
    33541 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    33601 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    33661 ttcgccaaat ccacaatgac ggctgcggct tcaaccggcg gcgtgccgcc ctggtgaatg
    33721 ttagagatac aggtgcgatc ggcctcaacg gtggtcgcca tacgcggcga gtaaacggcg
    33781 tagcaggaga ggctttctga ctgccctaac cccggacgtt cccccaccag caggatcacc
    33841 actttcgcgc cgaggatctc accgatctga tcttcaatct tcacgcgacc ataacgaaca
    33901 aagaatggcg tgccgacttt cagcccggcc tgtttcaggc ccgccatcag cggcggcagg
    33961 atctcttcgt agttcacggt gatggcatca gttgacaggc catcagaaat aacgacctgt
    34021 acgtccggat tagcaacaca ctgtgctttc agcgcttcaa cagcttctgc acacaggcgg
    34081 cggcccatat ccgggcgagt caggtagaga tttttgtcgc taatttcgga gcgtacttcc
    34141 agcaagccct gcgctttcac ccactcttcc ggcacttctt tcagtacggt atctttcgaa
    34201 cgagagtgat cggccaggaa acgcagcaac gcctgcgtac gcggacgcgg accggcacga
    34261 ccggtacaaa cgcgggccac ggtgctgcga cgcagttctg ttaatacgtc tgcgcgatgc
    34321 gggttttcaa caccaatcca cgcttttgct tccgcagaac ctaaatccag cgcgcagctt
    34381 tccgaggtca ccggtgccgc acagttggtg gtggcgcact ttgcttctga cggcgcgggg
    34441 gccgtttgtc ccattgacgc catcacgctg cgtacaattt cttcaatctg tttttgatcc
    34501 atgatgtgtt atctccgcgt catcagaaga atagtgacgg atcgcccgcc cgtttggtca
    34561 ggcgaccgtt tgccataatg cccatgcttt ccagccagcg ttcaaactcc ggtgacggac
    34621 gcaggttgag taactgacgc acagtggcgg tatcgtggaa tgcggtggtc tggtagttga
    34681 gcatgatgtc atcacccagc ggcatcccca tgatgtagtt gcagcctgcg gtggcgagca
    34741 ggatcatcag gttttcgttg aggttctggt cagcgtcagc gtggttggtg taacagcagt
    34801 cacagcccat agagatgccg ctcagcttgc ccataaagtg atcttctaag ccagcacgga
    34861 taatctggcg gtcgttgtag agatactccg gcccaataaa gccgaccacg gtgttgacga
    34921 taaacggatc gtaatgacgc gccagcccat agttacgtgc ttccatcgtc acctggtcag
    34981 cgccgaagtt agcgccagcg gatagcgcag agccttgtcc ggtttcgaag tagaggcagt
    35041 tttccccggt gatacggttg aactctgcac ccactgcgcg cgcttcgtcg agcatcgcca
    35101 gttccacgcc aaactctttc agcccttttt cgctgccaca gatactctgg aaaatcagtc
    35161 cgccaggcgc gccgcgacga atcgcttcga tctgggtggt gacgtgcgcc agtacgcagc
    35221 cctgagttgg gatgttgaat ttgtcgatca cgccgtaaat ggtgtccaac acgcggctta
    35281 agttttccac gtcgtcagtt accgggttaa cgccgatcac cgcatcgccc accccaaagg
    35341 aaagcccttc atagatttgc gcagcgatac tttgcacgtc gtcacgggta tcgttcggct
    35401 gcaaacgggc gctgaaggtg cccggaatac caatggtggt attggccttt ttgattaccg
    35461 gcattttctt cgcgccgtag atcaggtccg cgttggagca aatcttcgct accgccgcga
    35521 ccacttccga ggtcagccct ttgcgggtaa aggcaatgtc gtccacgctg gtttcatcgc
    35581 tcagcacata ctcacgcagt tcgctgatgc tccagttttt aatctggttg taggccgttt
    35641 cgttaacatc gtcctgaatc agccgcgtca cgcagtcatc ttcataggca atcaccggat
    35701 tattgcggat gtccgctacg gtcatttccg acaacacctg ctttgccgcc acgcgctcct
    35761 gtgagcttgc cgctgcaacg cccgccagca catcccccga acgcagttcg ttggctttag
    35821 ccagcacctc ttttacatcc ttaaactgat atacattgcc gaacaatgtg gtctttagtt
    35881 tcataagtcg ttccctcagg aaggaaatgc gagtgatttc accgtcaccg gcacaaccga
    35941 tccgccaaaa agaggcgtac caatgtcgat atagtccccc gcgcggacaa tcacttcgtc
    36001 aatgactgcc aacgggagtt gttgtagctg tgggcgcaac aacatgccca gagctttacc
    36061 aaagtcctgc ccggccacca ccagcagggg atgcggattc ggaaaacgcg cgacgaaatc
    36121 gaccagcgcg ttgatgaccg tcagtaccgc ggcgtaacgc acaggcagcg aggcgggaag
    36181 cgccagcacg tacgcgtcag ttttgggatc aagatccagc tgaagcagcg cctgttgcca
    36241 ggcactcacc agatccgttt catcaatcgg gatcgccacc ggcaaattgc gcagcggcag
    36301 ttgtacgccc tccagccaga ttgtgctgcc agagagcgaa agggtatgtg cacccgcgcc
    36361 aatcaccgtg gcgcgtacgg tttgcgccgg aaactgcaca ttcatctcac gcaggcgcgg
    36421 atggtcatgc agcgccgttg ccagaagtgg gccaatatcg gcaaaacaga acgggtcggc
    36481 gggctggtgg cgataacatt cgcccacgcc tccagaaagc gtaatgattt caggcgtaac
    36541 acctgcgggc agcaaaccgg tttgcatcaa tgcctgcgcg agcggcgaaa gcgttccgtc
    36601 aatcacttcg acaatcagcg ccgccatccg ccgggtaacc tgcaccagct gcgcgccggt
    36661 cagcgaacgg gcgtcagtgc ctgcaccgaa gcactcatcc acaatcatct gccccggttt
    36721 atgagcgtaa accacgcgcc cctggctgtc ggtttccagc aggcgaccac cgacgttgag
    36781 acaggcagtg ccgctgattt ttccggcatc gaacagggcg tagttcgcgg tgccaccgcc
    36841 gatgtcgata ttcagtaccc gacacagccg ttgttcagaa agggtttgcg ccccagctcc
    36901 gtgaccggcg atcacggatt cgaggtgcgg cccggcgctg gcaacgacaa aatcccccag
    36961 cgattgagag agcgccatca ccgccgggcg agcattgcgg gttttcgcgc tttcaccggt
    37021 gatgatgatg gcaccagaat caacgctttc cggcgcaata cccgcagcct gatattgctc
    37081 gagtattaag gttttcagtt ccgcttcttt taaaccgccc tgtttatcga caggggtaaa
    37141 gaacaccgga ctttgccagc taatttcgcg tttaatgaat tcgtagcgcg gcacctgcga
    37201 caccgccgca cggttaacca gctccaggtg ggagaaaatc acctgggtgg tggtggtgcc
    37261 gatatcgata ccgactctca atagctggcg agtgttcacg attgtgcctc cgcttcggtt
    37321 ttagtcgcgg tcgcgtcttc ttttggcacc agcatcatcg ccacgccaat cgccgttacg
    37381 ccgccgatca acttgccgac aatcatcggg aagatcatgg cgttcatgtt ggcagcggcg
    37441 aagcctaagt ggtcgcccag ggcgaaagca gcggaaacgg cgaaggcgca gttgatgact
    37501 ttgccgcggg tatccatctg cttcatcatg ccgaacatcg ggatgttgtt ggcaagcgtt
    37561 gccaccatgc cggctgccgc gatgttgttc atattcagca ctttaccgac gctcatcagc
    37621 ggtttttcaa accagcgagt cagcagcagc accattggat acgcccctaa cagcacgcag
    37681 gagatagaac cgataacttc aatggcgcgc atcacttcac cgggtttatc gccaggggcc
    37741 ataaagatag gatccagtcc ggggatcagt tcccagccaa ggaggaattt cactaccgca
    37801 gcggcaagac cgagggtgat caatgccacg aggaatttgg cgaagatctg gaagccgttg
    37861 atcatttttt ccgggatgaa tttcagcccc agcgccacca gaatcgcgac aatgatcacc
    37921 gggatcatgt tcatcaggat cagggcgaaa gtgaattcca ccggctgacc gttgatctgc
    37981 acaccggagt acatagcaac cagaccacca gcaatacagc caatcggaat ggtcacaatg
    38041 cccgccagca cgccgagcgc cagataacga cggtcagaag gttcgataat gccgagcgcc
    38101 accggaatgg aaaacacaat cgttggcccc atcatcgacc cgagaattaa cccagagtat
    38161 agccacgctg ctacgtcgcc gcccgccagc tctttggcga ggaagaagcc gcccatatcg
    38221 cacgccagca gtgttccggc gaacatcgat gggttagcgc cgagcatttc gtaaaccgga
    38281 ataattaccg gcccgagtac gtgagccagt accggtgcca gcgcggtcat accgaccatc
    38341 gccaggccca gtgcgcccat tgccataaag ccttcttcga actgaccgcc tgatccttcg
    38401 atacttttac cgaacttacc gaggaaacga gcagaaccgc cgaactgcga caggatcctg
    38461 tctacggcag ctatcagcat aaagaacatc atgatgtaca tgatgatttc gttaattccc
    38521 atcgccttta ctccctgtta gttgttattt attggcggat gcggcgtaaa cgccttatcc
    38581 gccctacatg tgcaatcccg aaggccggat cagacgcggc aagcgtcgca tccggcattt
    38641 attgcgccgc tgcgtacagg ccgacaatct gctccaggct ggcggtacgc gggttgctgc
    38701 gcagacaaat atcttcctgc gcggcctgcg cccatgcacc gtaatgcgca gatgtcgcgc
    38761 caacatcgcc cagtcgttta ccaatcccaa cttccgcaat cagctcactt accgcgttaa
    38821 tagcgtcacg atcgtcggat tttttagttc gcagtgcccg accaatctga ctaaaccgtt
    38881 cgcgacaaac catccggtta aattccatca ccgttggcag caacatggcg ttcgccagac
    38941 cgtgtggaat atgcagcgcc gcccctggct gatgcgccat cgcgtggcac aacccaagcc
    39001 ccgcactgga aaacgccatt cccgccatac atgaagccag taacatgctc tcgcgcgcgg
    39061 caaggtcatg accgtagccc accgctttcg gcagcgattt gccaatcatc gcaatggcac
    39121 caatcgccag actgtcggta aacggtgtag cgttcagggc gctgtatgct tcaatggcat
    39181 gggttaacgc atcaatgccg gtcatcgccg tgacatgcga cggcacacct tcggtcaatg
    39241 cggcgtcgag aatcgccaca tccggcatca gcgaggcatg ggctaacacc tgcttacgcc
    39301 cgctcaccgc atcgataatc accgttacgt tggtggtttc agagccggtt ccggcggtag
    39361 tgggaatggc aatcaacggc aggcgcgatt gcagaacgct ggtttctgac atctctgcca
    39421 gcgtgctatc cgggttcgtc accagcaacg ccacggcttt cgccgcatcc agcaccgagc
    39481 cgccgccaaa tgcgatcacc ccatcacagc ctgactcacg caactgcgcc acggctgcac
    39541 acacgtcggt gatgcacggt tcgcccaccg gacatggcca gagcgtcatg gcgatacctt
    39601 taacggccag gctacgcgtc agcccggcgg tcatccctgc ctgatgcaaa aagctgtctg
    39661 ccatcacgaa cagatgtttc agcccacgcg tttgcgcttg ctgtccgcaa ctgctcaccg
    39721 agcccggacc gcaaagcgtc accggtggaa cgctaaatgt ttttacccgt tgcagattca
    39781 gggtatcgaa cgcctgaaag agcgcggtct gcaattcatt ttgcatatag cccctccgct
    39841 ttctctcgcc cactactggc gatcgccagc ggggtcataa acaaactgtg ctgcggtaaa
    39901 tgcacctgta acgccgggaa ttgtttgcga aacagttccg ccacgcccgg ttgcatacaa
    39961 gagccgcccg ccagccataa atcagtaatg ccctgccctt caatatgccg ggcgacaatg
    40021 tccgccattt tttcgtacac cggtttcacc gcgggccaaa tctcgtcacc atgaccgcgc
    40081 ttgtactgct ctgcctcttc cagcgaaata cgacgatttc cggcgagagt cagagagatg
    40141 tgatgtccgc cggtggcttc atccgccgag tacgtcacct tgcccttttt cacgatggca
    40201 atgccggtag tgccgccgcc gatatccact acaccggcgt tgtccagttg cagcagatcc
    40261 gcgactgcgg ttggctcatc cagcacatgg ctcacttcca gcccggcaga ttccagcacg
    40321 ttgatggaaa tacgcgggtc cgtacctgga ggaaatgaag tcgccgcatg gctaaaacga
    40381 agaccgaatt gctgctcaag agtgtcgaga tggcgacgaa caatggtgac cgcaccgaag
    40441 aaatcccaga cgatgccgtc gcggaccaca tcggcccagt cgaggcatac cgccaccggc
    40501 tgaccgtcgc ggtcaacaac catcgacacc acatcgcagg tgcccagatc cacccccagc
    40561 cacagcggag attccgtcgc ggcaggcgtc tggttacaca gcgttgccgc cgtttgcaga
    40621 cgtggggtga gccattgttc gtcgtgcgcc atcggttact ccttaaacaa tgcgaaacgc
    40681 atcgactaat acacagcgac gcagacggac aaacgtccgc gcgctggtta ccccttcacc
    40741 ggttggcgtg gtgatggtca tggtggtcca gccttccccg cccagcccca gcccggcaat
    40801 gcacggtccg ttcttaacga aaatgctggt atcaatggcg ttcgccatct ggttcatgtt
    40861 ttcgatattg cgcgagtgca ttgccgccgt gtggtggcaa ccgccctcca gtttcaccgc
    40921 cagcgcaatg gcatccgcca cgttggcgac gcgcacgacg ggcagcaccg gcatcatcag
    40981 ttcggtcacg gcaaacggat gttctgcagt ggtttccaca aacagcaaac gcgtttcttg
    41041 cggcacgttc aggccgattg ccgccgcgat tttcgccgca tcgcgaccaa cccagtcacg
    41101 gctgacggtg ccttttccgc gctcgtcgat atttttcagc aacaccggtt gcagctgctg
    41161 cgcctgttct gcggtcagtt tcaccgcgtg ctggccttcc atcagacgca tcagttcatc
    41221 ggctacgcta tcaacaacaa tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa
    41281 agaagcgcct ttgacgatgg actgagcggc acgggcgagg tcggcggttt catccaccac
    41341 taccggcggg ttgccagcgc ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc
    41401 ttctactacc gcttcgccgc cggttactac cagcaggccg atacccggaa acttgaacaa
    41461 gcgttgcgcg gtttcgatat ccggatttgc cacagtaacc agtaagtttt ccggcccacc
    41521 tgcggcaaca atcgcctggt tgagcagcgt aatcgcccgc tgggagactt ttttcgccgc
    41581 cggatgcggg gcaaaaatga cgctgttgcc cgcggcaatc aggctgatgg cgttgttaat
    41641 tacggttgcc gccgggttag tggaaggcgt caccgaagcc accacgcccc agggtgcgtt
    41701 ttcaattagg gtcaggccgt tgtcgccagt cagcacttgc ggagagaggc actcaacgcc
    41761 tggtgtgccg cgcgcctgag cgacgttttt tgcaaattta tcttcaacgc gccccatgcc
    41821 ggtttcactg acggcaagtt ccgctaaatc tctggcgtgt ttttcgcctg cttcacgaat
    41881 ggcagcaatg gctaactggc gcattgccac gctttttaac ccttgctggg cgactttggc
    41941 tgccgcaacg gcgtcatcca gggacgcgaa aacgcccatc tcatgaacgg cggcggacgg
    42001 cgtgtcactg ctttgcattt tcagcagtac cgctttcacc acctgttcaa tatcctgttg
    42061 attcatgatg ttctgcctta tttgtggaaa attacctgac cgccagacac cacctcatcg
    42121 acaatgccaa tcacgcacag atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg
    42181 gaactgccac tcaccagtaa cacccactcc ccggttcccg cgccaatatt gtcgatggcg
    42241 acggcgcatt gcccgtcagg attaccttgt ggatcaatca tttccaccat cagcaatttg
    42301 tcatgcgcca gtccgtgatg gcgtacggta caaacaattt gtccagtgac gactgccagt
    42361 ttcatacccg cctccgtggc gtatttcagg taaaagctcc ccctaccctc cgcagaaggt
    42421 aaaatgaaaa aggagagagc gtgacgcccg aatcgacgtc acacagggtg attacaggtt
    42481 gctgctatcg cctttcaggc cgatcgggaa gacttcttcc aggtcgccgt gcggacgcgg
    42541 gataacgtgt acagatacca gctcgccgat acgctgtgcc gctgcggcac ctgcatcggt
    42601 cgcggcttta caggctgcaa catcaccacg aaccatggca gtacacaggc cgccgccaat
    42661 ctgcttaaca ccaaccagct tgacgcgcgc agctttcacc atcgcgtcag aagcctcaat
    42721 cagtgcaacc aggccccggg tttcgatcat tcctaatgct tccattgtgc tttcctcttt
    42781 atcagggtcc agaacgggac cgttcattca accagtgttt gtaaactgct ttcgcggttc
    42841 acttctgtct gacgcggcac agctgccacc agcgccagct cgataatttc ctgcacgcta
    42901 caaccacgag agagatcgtg catcggcgcg gcaagtcctt gtatcagtgg cccgacggca
    42961 cgatatccgc cgagtcgttg tgcgattttg taaccaatat ttccggcttc cagcgacgga
    43021 aaaaccatca cattggcctt gccctgtagc gggctggcag gcgctttttg cgccgccact
    43081 tccggcacga aggcggcgtc aaactgtaac tcgccatcca ccaccagctt tggtgcgcgc
    43141 tcacggacga tttctgtcgc ctgctggacg ttagcaacac aggggtgacg ggcgctacca
    43201 ttgctggaaa acgacagcat cgccacgcgc ggctcttctc cggtgatggc gcgccaggtt
    43261 tcggcactgg caagcgcgat atccgccagc tgcgccgccg tcggctgtgg caccacgctg
    43321 caatcggcaa agcccaacgc cgggccgctg tactgtggca acatcaggaa aatggaagag
    43381 agcgttttac agcctggctg caagccgata atgcgtaatc cggcacgcag cacatttgcc
    43441 gtggaagaga ggttgcccgc gatacagaca tccgctttac cggcgctgac cattgctgcg
    43501 gcgaacatca gagggtcggt aagtttttcc agcgcatccg gcggcgtttt ttcgcccgcg
    43561 cgggccagcc agcgatgagc aaattcttcc cgcattgcga ggttgccatg cggatcaatc
    43621 acctgtagcc cgtccatcgc cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga
    43681 ttggcgacca gaatgggcgt tgccagacct tgttgatgta aatattgcgc ggctttcagc
    43741 acacgttggt ctaacgcatc cggaaaaacc actctggcgg gcgctcgcaa cgccagttca
    43801 cgacaacgtt caataatcat tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta
    43861 gcggctgctt cgttacgctc aggatcatca tcacgtagac cgtgctggaa aggcggttca
    43921 gcgcctgcaa aatgtccggg cgtaatactt caaagctgcg ggtgataaac acctgcgccg
    43981 ctacggtttc ggtttcccgc actttggtac gtagcagatt gagcaacgcc gcatcgcgcc
    44041 cgtggctggc ttccggcacc agatgatcgt ggtcgagata gcgcagcggc tggtgagaaa
    44101 gtcgatgcag atcttcgtca gaaagcccga caatcgcctg gcagcccagc ggttcaccca
    44161 gcgcatcggc gcgcataatg ttgccaagac gcgaacggat atccgccagc cacggctgcc
    44221 acggttccgc cagttcaatt tgcagccaca ctgccagcgc aatggtgctg tcgagaaccg
    44281 cgcgaaaacc cagacgcgga tcgcttttgg cgaccatttt ttccgccgac aggtgagtca
    44341 gcgtatccgg cttttttgcc accggctggc ggcacagttc gcagcacgcc tgcggatgtt
    44401 catcgctact ggtcagccca tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca
    44461 ggcggccctg ctcgtcaata aacttgatgc gcagatggcg gctttccagt aactcccggg
    44521 cagagggcgt caggcgactg tccgcaggga gatggatctc tgcgccttcg ctgagcgtat
    44581 ggttcgctct tagccatgct tcggtgatga aatctttcat agggattgcc agttagccgg
    44641 ccaggcgaca tacaggaatt tcacgctgga tgtcgtacca aattcgatac tggagccttt
    44701 cgggataaac atcacgtcac ccgctttggc gatcatggtt tcgccttcgt ggcgaacatg
    44761 cagctcccct tccagcacca tgtcgatttc gtcgtagttc agggtccacg ggaagaatgc
    44821 gttctcccac tgcataaaac cagcggccat gctgctgccg tcgtctccgg tcacaagatc
    44881 agtcaagccc acgcaatgcg gctctgcgcc gtcgaaacga ccaaacttga cgctgctgcc
    44941 atcaatcact ttgatgccgc ctttgccggt caccgatttg aagctcggct gcattgcgcc
    45001 ctgctccagc gactgttttt ccttcatcac tttttccatc agctgtgcga ccaggctttc
    45061 ggtaaactgg ccttccggca gctgggcaat gatggtttcg cggatgcgct ggctttcggt
    45121 tttgtcggct ggcacgctgg cgggcacgga cgccgttacc gggatcgatt catcacattc
    45181 ggtgatggta aaacccagta gatccgccac ttcacgggct tccggggtaa taatgctggc
    45241 gcgcagaacc acggacattg cctgttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc
    45301 tgtgatgagt tttttcacct gcttcctcct gtttggtgag agatgccaga taatccacca
    45361 gttgctgcac gctttgcggg tcgtggctgt ttagctcgaa aatcggctct tcgaaacccg
    45421 tctcaagcaa taactttcgc gttgcggcga cgtcagcatc tggcatgtcc gttttactga
    45481 tgacggcgat ttgccgtttg ctgacgccaa tatccagcaa cccggcaggc aaccgacttt
    45541 ccgggtcatt tgcgccatgg acatagatca gcatgtctac atcctgtagc gtggtaatta
    45601 aggcgtgata ccagcgggga tggctaaaat attcacccgg cgtatcaata tcgcccttat
    45661 cattaaattc cacggcctgt gtttttctgg cgagggtata attcccctgc aacgcattaa
    45721 ataacgttgt ttttcctgcc ccgacggtac ccacaaacgc aattcgtttc atgaatgcct
    45781 cgtattaact tttggtcatt tcacaaagcg tataatttaa taagcgacct aaaccgctga
    45841 cggtttgtga taacgcttct tctactgcgc ccaccgagcc ataaatcacc aatgcaccgc
    45901 tgaagcggtc gagaaaaccg atatgcacat cagcggcttt cagcgctaaa tcaccggcga
    45961 tcatcgccgt ttcgccggga gttagcgtca taatgccaat tgcgcccgca tccggaacgc
    46021 cgattttctt cgccagttcc tcaccgggat gcgcgatgag atgcgccagt gtgacctgtt
    46081 tgccgggcac aaattcctga atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa
    46141 ctttcgtgta tcgtgacaat ttttcgtcag agtaaataac aaaattcggc aagcgcgttt
    46201 aaaaggtgag gtagatcact aagaatggag agatattcgc tgtggtgcat aaactttcat
    46261 atgcaacgca tgaatataaa taaaataata tcgagatatt atatagactg ctttttttat
    46321 tattcgcaaa ctcagtaata agctttacta atatattgca gccaaaccgc ttaccctgaa
    46381 tattcagggt aagcgtgaga gttaaaaaag aattacagcg gttgggtttg cgcttcaacc
    46441 acggccagcg ccaccatgtt gacgatacga cgcaccgatg cgatcggcgt taacacgtga
    46501 accggtttcg caacgcccat cagcaccgga ccaacagtca caccttccga gctggaaacg
    46561 cgcagtaagt tgtaactaat gcgggcagct tccatgttcg gcatcaccag aatattggca
    46621 gaacctttca aagggctgtc cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca
    46681 tcgccgtgca tttcaccatc aatcatcaga tctggcgcac gatccctgac cagttccagc
    46741 gcctgacgca ttttgctcga cgacgggcag tcagaagaac caaagttgga gtgcgacaac
    46801 agagcaacgc gcggctcaat accaaaacga cggacagttt ctgccgccat caaggtgatc
    46861 tccgccagct cttctgcgtc tggttcgtca ttgacgtagg tatcggcaat aaaggtgtta
    46921 ccactcggca gcagcagcgc gttcattgca cctgcggtgt gaacgccatc gcgataacca
    46981 aagacatttt tcaccacgct aaaatgttca tgataatcgc ccaccgtacc gcagatcatt
    47041 gcatcggctt ccccacgctg aaccatgatc gcgccgatca ctgtcgggtt actgatcagc
    47101 gcccgctgcg cctgttcctg agtgacaccg cgacgcttca tgatctggaa gtattcggtc
    47161 cagtactctt taaagcgcgg atcggattcg ttattgacga tctcaaaatc aacgcccgct
    47221 ttgatctgca agcccagttt ctgaatgcgc atttcgatca cgttcggacg accgataagg
    47281 atcggtttcg ccagtcccag cgttaccagt tcctgagtgg catgcagaac gcgcgcctct
    47341 tccccttctg gcagaacaac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc
    47401 ataaacaggt tggttttgta aacgaactca gtcagcttgt cgatgtagac gtcgaaatca
    47461 gcaatcggac gcgtcgccac gcccgactcc atcgcggctt tagcgaccgc aggagcgatc
    47521 ttaacgatca agcgcggatc aaacggtttt ggaatgatgt attccggacc aaagctcaga
    47581 tcctgatcgc catacgctga agccaccact tcgctctgtt cggcgtgggc cagttccgca
    47641 atcgcgcgta ccgccgccag tttcatctct tcattgatag ccgttgcgcc aacatccagc
    47701 gcgccacgga agataaacgg gaagcaaagg acgttgttga cctggtttgg atagtcagaa
    47761 cgaccggtgc aaatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc
    47821 ggttccgggt tcgccagcgc caggatcatt ggcgcacgag ccattttctt cactatttcc
    47881 tgggtcagca ctttcgggcc ggaacatcca aggaaaatat ccgcgccttc aatcacatca
    47941 tcgagggtac gtttgccgtc atccaccacc gcatatgcgg ctttggtttc cgccatgttt
    48001 ggctcacggc cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc
    48061 agacccagcg ctaccagcag gttcatacag gcgattgctg cggcacccgc gccggaaacc
    48121 accatccgca cgtcggagat gtttttctcc accacgcgca agccgttgag gatggcggca
    48181 gtgctgatga ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg
    48241 cgcagtttct gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgattccg
    48301 ccgaaggtcg gttcgagcgc agcgacaact tcaataaatt tgtccgggtc gagttcatca
    48361 acttcaatgt caaatacatc aatcccggcg aatttcttaa acagaacgcc cttgccttcc
    48421 atcaccggtt tgccagccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta
    48481 gagatcaccg ccaccaggtt accgcgcgcg gtatatttgt aggcttttaa cgggtctttt
    48541 tcgatttcaa gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc
    48601 gttgccagag gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa
    48661 tcaagtgcac tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac
    48721 aaataaagtg tgtgggcaac agcccctgcc cacaacgtgg cgcacattat taccctgccg
    48781 gagtctacag actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa
    48841 tcctataaca ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt
    48901 tttgatgcgt ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta
    48961 cttttcaagg agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg
    49021 tggcagacag cggcgatatt gagtccattc gccattatca tccccaggat gccaccacca
    49081 atccttcgct gttactcaaa gctgccggat tatcacaata tgagcattta atagacgatg
    49141 ctatcgcctg gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg
    49201 acaaactggc agtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt cgcgtgtcaa
    49261 cagaagttga tgcacgcctc tcttttgata aagaaaagag tattgaaaag gcgcgccatc
    49321 tggtggactt gtatcagcaa caaggcgttg agaaatcacg cattttgatc aaactggctt
    49381 cgacctggga aggaattcgc gcggccgaag agctggaaaa agaaggtatt aactgcaacc
    49441 tgacgctgct gttttctttt gcccaggcgc gggcctgtgc agaagcgggc gtttttctga
    49501 tttcgccgtt tgtcgggcgt atttatgact ggtatcaggc acgcaagccg atggacccgt
    49561 atgtggtgga agaagatccg ggcgttaaat cggtgcgcaa tatctacgac tactataagc
    49621 aacaccacta tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg
    49681 ccttaaccgg ctgcgatcga ctgactatcg caccgaattt gctgaaggag ctgcaggaaa
    49741 aagtttcgcc agtggtacgt aaattaatcc cgccttctca gacgttccca cgcccagcac
    49801 ccatgagcga agcggagttc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac
    49861 tgtctgaagg cattcgtctg ttcgctgttg atcaacgcaa actggaagat cttcttgccg
    49921 ccaaactata aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt
    49981 cgcgcactca gtatggatgc ggtacaaaaa gccaattctg gtcatcccgg cgcgccgatg
    50041 ggcatggctg atattgccga agtgctgtgg aacgattttc ttaaacataa tcctaccgac
    50101 ccaacctggt atgatcgcga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc
    50161 tacagtttgc tacatctgac cggttacgac ctgccgctgg aagaactgaa gaacttccgt
    50221 cagttgcatt cgaaaacccc aggccacccg gagattggct atacgccagg cgttgaaacc
    50281 accaccggcc cgcttggaca aggtttggcg aacgccgtcg ggctggcgat agcagagcgt
    50341 acactggcgg cgcagtttaa ccagccagac catgagatcg tcgatcactt cacctatgtg
    50401 tttatgggcg acggctgcct gatggaaggt atttcccacg aagtctgttc gctggcaggc
    50461 acgctgggac tgggcaagct gattggtttt tacgatcaca acggtatttc catcgacggt
    50521 gaaacagaag gctggtttac cgacgatacg gcaaaacgtt ttgaagccta tcactggcat
    50581 gtgatccatg aaatcgacgg tcacgatccg caggcggtga aggaagcgat ccttgaagcg
    50641 caaagcgtga aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg
    50701 ccgaataaag caggtaagga agaggcgcac ggcgcaccac tgggggaaga agaagtggcg
    50761 ctggcacggc aaaaactggg ctggcaccat ccgccatttg agatccctaa aaagatttat
    50821 cacgcctggg atgcccgcga aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt
    50881 gccgcctata aaaaggctca tccgcaactg gcagaagagt ttacccgtcg gatgagcggt
    50941 ggtttaccga aggactggga gaaaacgact cagaaatata tcaatgagtt gcaggcgaat
    51001 ccggcgaaaa tcgccacccg taaggcttcg caaaatacgc ttaacgctta cggaccgatg
    51061 ctaccggagc tgctcggagg ttcagcggat ctggctccca gcaacctcac catctggaaa
    51121 ggctctgtgt cgctgaagga agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa
    51181 tttggcatga ccgctatcgc caacggcatt gcgcaccacg gcggctttgt gccgtatacc
    51241 gcgacgttcc tgatgtttgt tgaatacgcc cgtaatgccg cgcggatggc ggcactgatg
    51301 aaagcacggc agattatggt ttatacccac gattctattg gcttagggga agatggtccg
    51361 acgcaccagg ccgttgagca actggccagt ctgcgcttaa cgccgaattt cagcacctgg
    51421 cgaccgtgcg atcaggtgga agcggcggtg ggctggaagc tggcggttga gcgccacaac
    51481 gggccgacag cactgatcct ctcaaggcag aatctggccc aggtggaacg tacgccggat
    51541 caggttaaag agattgctcg tggcggttat gtgctgaaag acagcggcgg taagccagat
    51601 attattctga ttgccaccgg ttcagagatg gaaattaccc tgcaagcggc agagaaatta
    51661 gcaggagaag gtcgcaatgt acgcgtagtt tccctgccct cgaccgatat tttcgacgcc
    51721 caggatgagg aatatcggga gtcggtgttg ccttctaacg ttgcggctcg cgtggcggtg
    51781 gaagcaggta ttgccgatta ctggtacaag tatgttggtc tgaaaggggt aattgtcggg
    51841 atgacgggtt acggggaatc tgctccggcg gataagctgt tcccgttctt tggctttacc
    51901 gccgagaata ttgtggcaaa agcgcataag gtgctgggag tgaaaggtgc ctgatggtga
    51961 tcgccggatg ctgattgccg gatgcgacgc tgacgcgtct tatccggcct acagcgtctt
    52021 atccggccta catgtccccg caattttgtt taacgggtga tccacaacgt gggccaggcg
    52081 tctggcccat gccagttatc gcaggtgggt tctgcggcat aacgcaccag gcgaaaacgc
    52141 tgaccgtcaa agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct
    52201 aaggtcacca gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc
    52261 gtctcctgac cgttgttgaa tggcaaacgc aaccgaaccg ggcgcgaggc tagtggcttt
    52321 ttacgcgaca caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc
    52381 atcatcagcg ctttatcatc agtcagcgca gtgacattca cctcacgacg taacggatca
    52441 agcgagcagc gcagaccatt catccgccag tttccataat ccagcaaatc gttgcgttct
    52501 tcgagtgaga gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgcg
    52561 ggcggtacgc tgagcggcgg ttcgtcccct ttcttgatcc acgcggtttc actgccaacg
    52621 cgcttttgct gagcatcaat aaacaacaac gccgctttta agccactcag agaaatggtc
    52681 tgattgccgt cgcgtaaggt gatcgccttc ccttcctgaa tcatctgcaa aaacgcggtg
    52741 atggttgccg tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt
    52801 aacgccaacg gctcaccatc taacagcagc cgtggcgcta tctctccttc agacgcatcc
    52861 ggcgacttca acccgccgcg ctcaatacgt aaaacggcat cggtatgcgc cccggcgctg
    52921 cggctcaggg tcatcaccag tccattatga tcgcccgtat tgcgcgccac gcagaaattt
    52981 tggttattac aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc
    53041 aaagacgtcg gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt
    53101 acgaccccag aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg
    53161 ctcaatcaga tttgtcggat tgagccaata ataaacaacg aaatattatt gttatttttc
    53221 agtccattaa atgtgacgtt tgcaaatagt taagcaataa caccgtctta ccgtcacgta
    53281 tctcgccggt tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat
    53341 cttcatcttc aacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa
    53401 aaaagtggat tagctcagtc acaccgccag gcgacatata cagttcaaat aatttgcgca
    53461 cttcgcccac ttcatagccc gtctcttcaa tcgcttcttt acgaatgcac acttccggtt
    53521 cgtcgttatc cagcagcccg gcgcaggttt caatcagctg cccgctttca ttgccattaa
    53581 cccaggtagc gacacggaac tgacgaatca gaaccacgct ctttttcttc gcgttgtaca
    53641 ggaggatcgt cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc
    53701 catctttgcg cgtgagatcg taggtaatgt tgtgcagggt gaaatagtta tcggagagaa
    53761 ttttgtcttt aatgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgagca
    53821 attatcttac gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat
    53881 tttaccccca gccagtcaat aatcccctgt gccgcatgac gtccttctgc cattgcggta
    53941 accaccagat ccgcaccacg cacggcgtcg ccaccggcga agattttcgg attggtggtc
    54001 tggtaacggt actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca
    54061 ccgtgcgact ccagccacgg catcccgtgt ggattgaagc caaacgccat aatcaccgca
    54121 tcggctggca tgacaaattc actgccctca atcggtacag gtcgccgacg cccctgggca
    54181 tccggctcgc caagacgcgt gcgcaggaaa cgaatcccgc agacgtgacc ttgttcattc
    54241 agctcaagcg ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc
    54301 ctcacctctt tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg
    54361 ttactcgcgc cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc
    54421 agtaccacga cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc
    54481 cccatcacct gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac gcccggcgca
    54541 tcttcattgg gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg
    54601 tcgtattgtt ccaaaagcga atccaaagag acatctttac ccacttcaca attgagttcg
    54661 aagtgaatcc ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt
    54721 ttgaaagaag gaatgccgaa agtgagcaag ccaccgattt ctggatggcg atcgtacacc
    54781 gtcaccgcca cgccattgcg ggtcagaaca tccgcacagg ccagccctgc cggacctgcc
    54841 ccgataatcg ccacccgctt gtccactttg gtgacatgac ttaagtcagg acgccaacct
    54901 ttcgccaacg cctgatctga aatatagcgt tcaatgttgc cgatagttac cgcgccgtgc
    54961 tcatcgcgaa tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta
    55021 atttccggca gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct
    55081 ttcaccagtt caatccactg cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg
    55141 ctgtgctcgc cgcacttaag gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat
    55201 ggcagataaa tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atccggctcg
    55261 ccacgcgcgg gcgttgcctg catttgttcg actttactca ttaccggcat ttcttgcgcc
    55321 gcggtactgg catgccacgg ttgatgttcc tgacgcgcgg tgcgcaagcg gcgggatttc
    55381 gccatgccgg agagtgcgac gtcagtgacc agttgcagcg cgtccgccgg gcaattctca
    55441 acacaggcag gaccgttttc gcgccccgca caaaggtcgc atttatgcgc cgtggctttt
    55501 acttttcctg ccgcgacggg tgtcaggacg atttgcatcg taccaaaagg acaggccacc
    55561 acgcaggatt tacagccaat acacttttgc tgattgacct gaatgctgtc atcaacgtgg
    55621 ctgattgcgc cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc
    55681 actgcactac gttgctgttg atgtttgata accgtaattc ggggatgaaa atggtgttgg
    55741 ctcaggacat gttgctcatc attgtgagcc atgacacagg cgatttcaca agcatgacaa
    55801 cccagacatt gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt
    55861 tgtaaaaacc ttattcttta tatgagtgtt gttattaccc gacttacagg ggaatcggca
    55921 atgttcatgt gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc
    55981 ccatcactga ctaaattgtg tttcagctaa ctggaacatt aatgattttt tgtggagaag
    56041 acgcgtgtga ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt
    56101 gtgctgctgt cgattctttc cacgggtatc gctctgctaa ctctggcgag cagtttgcgc
    56161 gacgctgagg ctatcaatat tgccggatcg ctacgtatgc agagttaccg cctgggctac
    56221 gacttgcaaa gtggcagtcc acaactcaat gcacatcgcc agttgtttca gcaggcactg
    56281 cattcaccgg tattaaccaa tctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc
    56341 tatgcgcatc tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgatttg
    56401 ccgtggtatc aggccaatat taataattat gttaatcaga tagacctgtt cgtgctggct
    56461 ttacagcact acgctgaacg caaaatgctg ctggtggtgg cgatttccct ggctggcggt
    56521 atcggtattt tcacgctggt cttttttact ttgcgccgta tacgccatca ggtggttgcc
    56581 ccgctgaatc agctggttac cgccagtcaa cgtattgaac acgggcagtt cgactcgccg
    56641 ccgctggata ccagtctgcc gaatgagctt ggtctgcttg caaaaacctt taaccagatg
    56701 tcgagcgagc tgcataaatt gtacctttcg ctggaggcgt cagtagaaga aaagacccgc
    56761 gatctccacg aggccaagcg tcgtctggag gtgttgtatc aatgttccca ggcgctgaac
    56821 accagccaga ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa
    56881 gcggctgaat atctggagtt aaatgtcggt gaaaactggc ggattagcga agggcaacca
    56941 aacccggaat tgccgatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa
    57001 ctgcactggc aaaatagtca cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg
    57061 atgctgggac gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg
    57121 atggaagaac gtgcaaccat cgcccgcgaa ttgcacgact cgctggctca ggtactttct
    57181 tacttacgta tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca
    57241 caaagtatca tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag
    57301 ctgttgacta ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa
    57361 atgctggata cgttacaaaa tcaaaccagc gccaaactga ccctcgactg ccgtctgcca
    57421 accctggcgc tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg
    57481 ctgaatgcga tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg
    57541 gacggcaatc acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa
    57601 cccgaaggtc attatggtct gaatatcatg cgcgaacgcg cggaacggct gggtgggacg
    57661 ctgacttttt cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctctgcg
    57721 gagggtgagg aaagtcagtt gatgtaatgc ctcctactga ccaaagaata cttgcactta
    57781 aggttcagta taaaagggca tgataattta cattaactcc tttttttctc cacgattggc
    57841 tcgtaccttg ccgctacagt gaagcaagtc aagcctacaa cgatacgcag aaacacgagg
    57901 tcctctttta atggcgaatt tctttattga tcgccccatt tttgcctggg tgctggcaat
    57961 cctgttgtgt ctgacaggga ccctggcgat tttttcattg cccgttgaac aataccccga
    58021 tctcgcgcca ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct
    58081 ggaaaacacc gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctcatgta
    58141 tatgtcatct cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg
    58201 caccgatccg gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa
    58261 gttaccgcag gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaatat
    58321 tctgaccatt gctttcgtct ctaccgatgg ttcgatggat aaacaggata ttgccgatta
    58381 tgttgccagt aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc
    58441 ctatggttcg caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca
    58501 gatgacggct aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg
    58561 gcaacttggt ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca
    58621 gtcactgctg caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaagacgg
    58681 ctcagaggta aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta
    58741 tcttagccgc ttcaatggta agccagcctc cgggctgggg gtaaaactgg cctccggcgc
    58801 aaacgaaatg gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtactt
    58861 cccgcacgga ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat
    58921 tgaagacgtg gtgaaaacgc tgctggaagc tatcgccctg gttttcctcg ttatgtatct
    58981 gttcctgcaa aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat
    59041 gggaaccttc tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc
    59101 gatggtactg gcgatcggtc tgctggtgga tgacgcaatc gtggtggtgg aaaacgtcga
    59161 acgtattatg agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca
    59221 gatccagggg gcactggtcg ggattgcgat ggttctttcg gcggtatttg taccaatggc
    59281 cttcttcggc ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc
    59341 gatggtgctg tcagtactgg tagcgatgat cctcactccg gctctgtgtg ccacactact
    59401 taagccactg aaaaaaggtg agcatcacgg gcaaaaaggc ttttttgcct ggtttaacca
    59461 gatgtttaac cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag
    59521 cctgcgctgg attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg
    59581 tttgccgacg tcgttcttac cgctggaaga ccgtggcatg tttactacct cggtacaatt
    59641 gcccagcggt tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta
    59701 cttcacccat gaaaaagaca acatcatgtc ggtgtttgcc actgttggtt ctggccccgg
    59761 gggtaacggg caaaacgtgg cgcgaatgtt tatccgcctg aaagactgga gcgaacgcga
    59821 cagtaagacc ggcacctcgt ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat
    59881 taaagaagct cgcgttatcg ccagcagccc gccagcaatt agcggtcttg gtagttctgc
    59941 aggttttgat atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc
    60001 acgtaatcag ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa
    60061 cggcctcgac gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct
    60121 gggcgttgct atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt
    60181 gaatgacttt atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta
    60241 tcgcatgctg ccagatgaca ttaatctctg gtatgtccga aataaagatg gcggcatggt
    60301 gcccttctct gctttcgcga cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta
    60361 taacggttat tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac
    60421 ggcgatggat attatggaat cgttagtgaa gcagttgcca aacggctttg gtctggagtg
    60481 gacggcgatg tcgtatcagg agcgactttc cggcgcgcag gctccggcgc tgtacgccat
    60541 ttccttgctg gtggtattcc tgtgtctggc ggcgttgtat gaaagctggt cggtgccgtt
    60601 ctcggtaatg ctggtcgtgc cgctgggggt tatcggcgcg ctgctggcaa cctggatgcg
    60661 cgggctggaa aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc
    60721 gaaaaacgcc atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccacgacct
    60781 gtttgaagcg acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct
    60841 ggcatttatc ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca
    60901 gcatgcggtg ggtactggcg taatgggcgg gatgatttca gctactattc tggctattta
    60961 cttcgtgccg ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga
    61021 ataagcaata aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa
    61081 cctgctcagc gggttcttgc tgtttatact ttgtctcggg aattacttac gaagcataac
    61141 ttcgataaag tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat
    61201 tatgggtatt caacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat
    61261 gactacctcc gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat
    61321 gtgatacaca gcaacatttc gagatattca tacggcatct aatactgatt taattctggt
    61381 taaaatacag acagataaca agatgaatat tct
//
LOCUS       DAZGTR010000016        56966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGTR010000016 DAZGTR010000000
VERSION     DAZGTR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.016"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(138..401)
                     /gene="cedA"
                     /locus_tag="ACHLCY_001128"
     CDS             complement(138..401)
                     /gene="cedA"
                     /locus_tag="ACHLCY_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP0368435.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            584..2845
                     /gene="katE"
                     /locus_tag="ACHLCY_001129"
     CDS             584..2845
                     /gene="katE"
                     /locus_tag="ACHLCY_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP0368436.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3104..3862)
                     /gene="chbG"
                     /locus_tag="ACHLCY_001130"
     CDS             complement(3104..3862)
                     /gene="chbG"
                     /locus_tag="ACHLCY_001130"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP0368437.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3875..5227)
                     /gene="celF"
                     /locus_tag="ACHLCY_001131"
                     /gene_synonym="chbF"
     CDS             complement(3875..5227)
                     /gene="celF"
                     /locus_tag="ACHLCY_001131"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0368438.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5332..6174)
                     /gene="chbR"
                     /locus_tag="ACHLCY_001132"
     CDS             complement(5332..6174)
                     /gene="chbR"
                     /locus_tag="ACHLCY_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP0368439.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6182..6532)
                     /gene="chbA"
                     /locus_tag="ACHLCY_001133"
     CDS             complement(6182..6532)
                     /gene="chbA"
                     /locus_tag="ACHLCY_001133"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP0368440.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6583..7941)
                     /gene="chbC"
                     /locus_tag="ACHLCY_001134"
     CDS             complement(6583..7941)
                     /gene="chbC"
                     /locus_tag="ACHLCY_001134"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP0368441.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPVLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8026..8346)
                     /gene="chbB"
                     /locus_tag="ACHLCY_001135"
     CDS             complement(8026..8346)
                     /gene="chbB"
                     /locus_tag="ACHLCY_001135"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP0368442.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8645..8983)
                     /gene="osmE"
                     /locus_tag="ACHLCY_001136"
     CDS             complement(8645..8983)
                     /gene="osmE"
                     /locus_tag="ACHLCY_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP0368443.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9185..10012
                     /gene="nadE"
                     /locus_tag="ACHLCY_001137"
     CDS             9185..10012
                     /gene="nadE"
                     /locus_tag="ACHLCY_001137"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP0368444.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10242..11129
                     /gene="cho"
                     /locus_tag="ACHLCY_001138"
     CDS             10242..11129
                     /gene="cho"
                     /locus_tag="ACHLCY_001138"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP0368445.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11089..11664)
                     /gene="ves"
                     /locus_tag="ACHLCY_001139"
     CDS             complement(11089..11664)
                     /gene="ves"
                     /locus_tag="ACHLCY_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP0368446.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADHFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11867..12352)
                     /gene="spy"
                     /locus_tag="ACHLCY_001140"
     CDS             complement(11867..12352)
                     /gene="spy"
                     /locus_tag="ACHLCY_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP0368447.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPIMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIVASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12682..13650)
                     /gene="astE"
                     /locus_tag="ACHLCY_001141"
     CDS             complement(12682..13650)
                     /gene="astE"
                     /locus_tag="ACHLCY_001141"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP0368448.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13643..14986)
                     /gene="astB"
                     /locus_tag="ACHLCY_001142"
     CDS             complement(13643..14986)
                     /gene="astB"
                     /locus_tag="ACHLCY_001142"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP0368449.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPIRELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14983..16461)
                     /gene="astD"
                     /locus_tag="ACHLCY_001143"
     CDS             complement(14983..16461)
                     /gene="astD"
                     /locus_tag="ACHLCY_001143"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP0368450.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16458..17492)
                     /gene="astA"
                     /locus_tag="ACHLCY_001144"
     CDS             complement(16458..17492)
                     /gene="astA"
                     /locus_tag="ACHLCY_001144"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP0368451.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17489..18709)
                     /gene="astC"
                     /locus_tag="ACHLCY_001145"
     CDS             complement(17489..18709)
                     /gene="astC"
                     /locus_tag="ACHLCY_001145"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP0368452.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19155..19961
                     /gene="xthA"
                     /locus_tag="ACHLCY_001146"
     CDS             19155..19961
                     /gene="xthA"
                     /locus_tag="ACHLCY_001146"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP0368453.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20128..20838
                     /gene="ydjX"
                     /locus_tag="ACHLCY_001147"
     CDS             20128..20838
                     /gene="ydjX"
                     /locus_tag="ACHLCY_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0368454.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20843..21520
                     /gene="ydjY"
                     /locus_tag="ACHLCY_001148"
     CDS             20843..21520
                     /gene="ydjY"
                     /locus_tag="ACHLCY_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP0368455.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21535..22242
                     /gene="ydjZ"
                     /locus_tag="ACHLCY_001149"
     CDS             21535..22242
                     /gene="ydjZ"
                     /locus_tag="ACHLCY_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0368456.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLASIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22242..22790
                     /gene="ynjA"
                     /locus_tag="ACHLCY_001150"
     CDS             22242..22790
                     /gene="ynjA"
                     /locus_tag="ACHLCY_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP0368457.1"
                     /translation="MGLPPLSKISFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22800..23966
                     /gene="ynjB"
                     /locus_tag="ACHLCY_001151"
     CDS             22800..23966
                     /gene="ynjB"
                     /locus_tag="ACHLCY_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0368458.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNRRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            23939..25474
                     /gene="ynjC"
                     /locus_tag="ACHLCY_001152"
     CDS             23939..25474
                     /gene="ynjC"
                     /locus_tag="ACHLCY_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP0368459.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPTLLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25474..26127
                     /gene="ynjD"
                     /locus_tag="ACHLCY_001153"
     CDS             25474..26127
                     /gene="ynjD"
                     /locus_tag="ACHLCY_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0368460.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            26194..27501
                     /gene="ynjE"
                     /locus_tag="ACHLCY_001154"
     CDS             26194..27501
                     /gene="ynjE"
                     /locus_tag="ACHLCY_001154"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP0368461.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQQVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27510..28130)
                     /gene="ynjF"
                     /locus_tag="ACHLCY_001155"
     CDS             complement(27510..28130)
                     /gene="ynjF"
                     /locus_tag="ACHLCY_001155"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0368462.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            28217..28624
                     /gene="nudG"
                     /locus_tag="ACHLCY_001156"
     CDS             28217..28624
                     /gene="nudG"
                     /locus_tag="ACHLCY_001156"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP0368463.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREKLGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(28590..28862)
                     /gene="ynjH"
                     /locus_tag="ACHLCY_001157"
     CDS             complement(28590..28862)
                     /gene="ynjH"
                     /locus_tag="ACHLCY_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP0368464.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            29098..30441
                     /gene="gdhA"
                     /locus_tag="ACHLCY_001158"
     CDS             29098..30441
                     /gene="gdhA"
                     /locus_tag="ACHLCY_001158"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP0368465.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30558..31598)
                     /gene="ynjI"
                     /locus_tag="ACHLCY_001159"
     CDS             complement(30558..31598)
                     /gene="ynjI"
                     /locus_tag="ACHLCY_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0368466.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANIFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(31726..33687)
                     /gene="topB"
                     /locus_tag="ACHLCY_001160"
     CDS             complement(31726..33687)
                     /gene="topB"
                     /locus_tag="ACHLCY_001160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0368467.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(33692..34735)
                     /gene="selD"
                     /locus_tag="ACHLCY_001161"
     CDS             complement(33692..34735)
                     /gene="selD"
                     /locus_tag="ACHLCY_001161"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP0368468.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEIPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIH"
     gene            complement(34852..35403)
                     /gene="ydjA"
                     /locus_tag="ACHLCY_001162"
     CDS             complement(34852..35403)
                     /gene="ydjA"
                     /locus_tag="ACHLCY_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP0368469.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35564..37420
                     /gene="sppA"
                     /locus_tag="ACHLCY_001163"
     CDS             35564..37420
                     /gene="sppA"
                     /locus_tag="ACHLCY_001163"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP0368470.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37587..38603
                     /gene="ansA"
                     /locus_tag="ACHLCY_001164"
     CDS             37587..38603
                     /gene="ansA"
                     /locus_tag="ACHLCY_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP0368471.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            38614..39255
                     /gene="pncA"
                     /locus_tag="ACHLCY_001165"
     CDS             38614..39255
                     /gene="pncA"
                     /locus_tag="ACHLCY_001165"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP0368472.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHSSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39416..39688)
                     /gene="yeaC"
                     /locus_tag="ACHLCY_001166"
     CDS             complement(39416..39688)
                     /gene="yeaC"
                     /locus_tag="ACHLCY_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP0368473.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(39730..40143)
                     /gene="msrB"
                     /locus_tag="ACHLCY_001167"
     CDS             complement(39730..40143)
                     /gene="msrB"
                     /locus_tag="ACHLCY_001167"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP0368474.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGCLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            40485..41480
                     /gene="gapA"
                     /locus_tag="ACHLCY_001168"
     CDS             40485..41480
                     /gene="gapA"
                     /locus_tag="ACHLCY_001168"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0368475.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            41564..42448
                     /gene="yeaD"
                     /locus_tag="ACHLCY_001169"
     CDS             41564..42448
                     /gene="yeaD"
                     /locus_tag="ACHLCY_001169"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP0368476.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(42499..43353)
                     /gene="yeaE"
                     /locus_tag="ACHLCY_001170"
     CDS             complement(42499..43353)
                     /gene="yeaE"
                     /locus_tag="ACHLCY_001170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP0368477.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(43443..44189)
                     /gene="mipA"
                     /locus_tag="ACHLCY_001171"
     CDS             complement(43443..44189)
                     /gene="mipA"
                     /locus_tag="ACHLCY_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP0368478.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            44625..46559
                     /gene="yeaG"
                     /locus_tag="ACHLCY_001172"
     CDS             44625..46559
                     /gene="yeaG"
                     /locus_tag="ACHLCY_001172"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP0368479.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            46672..47955
                     /gene="yeaH"
                     /locus_tag="ACHLCY_001173"
     CDS             46672..47955
                     /gene="yeaH"
                     /locus_tag="ACHLCY_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP0368480.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPIFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            48102..49577
                     /gene="cdgI"
                     /locus_tag="ACHLCY_001174"
     CDS             48102..49577
                     /gene="cdgI"
                     /locus_tag="ACHLCY_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0368481.1"
                     /translation="MIQSTRISMGLFFKYCLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            49758..51248
                     /gene="dgcJ"
                     /locus_tag="ACHLCY_001175"
     CDS             49758..51248
                     /gene="dgcJ"
                     /locus_tag="ACHLCY_001175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP0368482.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILAALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            51291..51794
                     /gene="yeaK"
                     /locus_tag="ACHLCY_001176"
     CDS             51291..51794
                     /gene="yeaK"
                     /locus_tag="ACHLCY_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP0368483.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(51795..51899)
                     /gene="yoaI"
                     /locus_tag="ACHLCY_001177"
     CDS             complement(51795..51899)
                     /gene="yoaI"
                     /locus_tag="ACHLCY_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP0368484.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            52069..52515
                     /gene="yeaL"
                     /locus_tag="ACHLCY_001178"
     CDS             52069..52515
                     /gene="yeaL"
                     /locus_tag="ACHLCY_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP0368485.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(52472..53293)
                     /gene="nimR"
                     /locus_tag="ACHLCY_001179"
     CDS             complement(52472..53293)
                     /gene="nimR"
                     /locus_tag="ACHLCY_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP0368486.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            53390..54571
                     /gene="nimT"
                     /locus_tag="ACHLCY_001180"
     CDS             53390..54571
                     /gene="nimT"
                     /locus_tag="ACHLCY_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP0368487.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTVVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            54626..54973
                     /gene="yeaO"
                     /locus_tag="ACHLCY_001181"
     CDS             54626..54973
                     /gene="yeaO"
                     /locus_tag="ACHLCY_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP0368488.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(54995..55249)
                     /gene="yoaF"
                     /locus_tag="ACHLCY_001182"
     CDS             complement(54995..55249)
                     /gene="yoaF"
                     /locus_tag="ACHLCY_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP0368489.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            55432..56457
                     /gene="dgcP"
                     /locus_tag="ACHLCY_001183"
     CDS             55432..56457
                     /gene="dgcP"
                     /locus_tag="ACHLCY_001183"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP0368490.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHS"
     gene            56490..56588
                     /gene="yoaK"
                     /locus_tag="ACHLCY_001184"
     CDS             56490..56588
                     /gene="yoaK"
                     /locus_tag="ACHLCY_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP0368491.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            56591..56665
                     /gene="yoaJ"
                     /locus_tag="ACHLCY_001185"
     CDS             56591..56665
                     /gene="yoaJ"
                     /locus_tag="ACHLCY_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP0368492.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(56724..>56966)
                     /gene="yeaQ"
                     /locus_tag="ACHLCY_001186"
     CDS             complement(56724..>56966)
                     /gene="yeaQ"
                     /locus_tag="ACHLCY_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0368493.1"
                     /translation="ILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGGWI
                     STLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
BASE COUNT        14090 a        14700 c        13893 g        14283 t
ORIGIN      
        1 aagaaaaaag atcacttatt tatagcaata gatcgtcaaa ggcagctttt tgttactggt
       61 ggtttgaatg aatgtagcaa tgaaatacag aatttcaggt catgtaactc ccggcaaaac
      121 cgggaggtac gtaatcttta ctcagtcact tccccttcct ggcggatctg atttgcccaa
      181 cgctgggcgg attcaggcac agtaaacgcc ggtgagcgca gaaatgactc tcccatcagt
      241 acaaacgcaa catatttgcc acgcagcatc cagacatcac gaaacgaatc catctttatc
      301 gcatgttctg gcggcgcggg ttccgtgcgt gggacatagc taataatctg gcggttttgc
      361 tggcggagcg gtttcttcat tactggcttc actaaacgca tattaaaaat cagaaaaact
      421 gtagtttagc cgatttagcc cctgtacgtc ccactttgcg tgtatttcat aacaccgttt
      481 ccagaatagt ctccgaagcg ggatctggct gatggtctat agttagaggg ttttttgacc
      541 aaaacagcgg ccctttcagt aataaattaa ggagacgagt tcaatgtcgc aacataacga
      601 aaagaaccca catcagcacc agtcaccact acacgattcc agcgaagcga aaccggggat
      661 ggactcactg gcacctgagg acggatctca tcgtccagcg gctgaaccaa caccgccagg
      721 tgcacaacct accgcccctg ggagcctgaa agcccctgat acacgtaacg aaaaacttaa
      781 ttctctggaa gacgtacgca aaggcagtga aaattatgcg ctgaccacta atcagggcgt
      841 gcgcatcgcc gatgatcaaa actcactgcg tgccggtaac cgtggtccaa cgctgctgga
      901 agattttatt ctgcgcgaga aaatcaccca ctttgaccat gagcgcatcc cggaacgtat
      961 tgttcatgca cgcggatcag cagctcacgg ttatttccag ccatataaaa gcttaagcga
     1021 tatcaccaaa gcggatttcc tctcagatcc gaacaaaatc accccagtat ttgttaggtt
     1081 ctctaccgtt cagggtggcg ctggctctgc cgataccgtg cgtgatatcc gtggttttgc
     1141 caccaagttc tatactgaag agggtatttt tgacctcgtt ggcaataaca cgccaatctt
     1201 ctttatccag gatgcgcata aattccccga ttttgttcat gcggtaaaac cagaaccgca
     1261 ctgggcaatt ccacaagggc aaagtgccca cgatactttc tgggattatg tttctctgca
     1321 acctgaaact ctgcacaacg tgatgtgggc gatgtcggat cgcggcatcc cccgcagtta
     1381 ccgcaccatg gaaggcttcg gtattcacac cttccgcctg attaatgccg aagggaaggc
     1441 aacgtttgta cgtttccact ggaaaccact ggcaggtaaa gcctcactcg tttgggatga
     1501 agcacaaaaa ctcaccggac gtgacccgga cttccaccgc cgcgagttgt gggaagccat
     1561 tgaagcaggc gattttccgg aatacgaact gggcttccag ttgattcctg aagaagatga
     1621 attcaagttc gacttcgatc ttctcgatcc aaccaaactg atcccggaag aactggtgcc
     1681 cgttcagcgt gtcggcaaaa tggtgctcaa tcgcaatccg gataacttct ttgccgaaaa
     1741 cgaacaagca gcattccatc caggtcatat tgttcctggt ctggacttca ccaacgatcc
     1801 gctgttgcag ggacgtttgt tctcctatac cgatacacaa atcagtcgtc ttggtgggcc
     1861 gaatttccat gagattccga ttaaccgtcc gacctgccct taccacaact tccagcgtga
     1921 tggcatgcat cgtatgggga tcgacactaa cccggcgaat tacgaaccga actcgatcaa
     1981 cgataactgg ccgcgtgaaa caccgccggg accgaaacgc ggcggttttg aatcatatca
     2041 ggaacgcgtg gaaggaaata aagttcgtga gcgcagtcca tcgtttggcg aatattattc
     2101 ccatccgcgt ctgttctggc taagtcagac gccattcgag cagcgccata ttgtcgatgg
     2161 tttcagtttt gagttaagca aagtcgttcg tccgtatatt cgtgagcgcg ttgttgacca
     2221 gctggcgcat attgatctca ctctggccca ggcggtggcg aaaaatctcg gtatcgaact
     2281 gactgacgac cagctgaata tcaccccacc tccggacgtc aacggtctga aaaaggatcc
     2341 atccttaagt ttgtacgcca ttcctgacgg tgatgtgaaa ggtcgcgtgg tagcgatttt
     2401 acttaatgat gaagtgagat cggcagacct tctggccatt ctcaaggccc tgaaggccaa
     2461 aggcgttcat gccaaactgc tctactcccg aatgggtgaa gtgactgcgg atgacggtac
     2521 ggtgttgcct atagccgcta cctttgccgg tgcaccttcg ctgacggtcg atgcggtcat
     2581 tgtcccttgc ggcaatatcg cggatatcgc tgacaacggc gatgccaact actacctgat
     2641 ggaagcctac aaacacctta aaccgattgc gctggcggga gacgcgcgca agtttaaagc
     2701 aacaatcaag gtcgctgacc agggtgaaga agggattgtg gaagctgaca gcgctgacgg
     2761 tagttttatg gatgaactgc taacgctgat ggcagcacac cgcgtgtggt cacgcattcc
     2821 taagattgac aaaattcctg cctgatggga ggcggcgcaa ttgcgccgcc tcaaatgatt
     2881 tacatagtgc gctttgttta tgccggatgc ggcgtgaacg ccttatccgg cctacaaagt
     2941 cgtataaatt caatgtattg cagtttattg gtagatcctg ataagcgaag cgcatcaggc
     3001 agttttgcgt ttgtcagcag tctcaagcgg cgcagttacg ccgcctttgt aggaattaat
     3061 cgccggatgc aaggttcacg ccgcatctgg caaacatcct cacttacaca tcccgataac
     3121 tccccaaccg ataaccacgc tgagcgatag cacctttcaa cgacgctgat gtcaacacat
     3181 ccagctccgt taagcgtggg aaacagtaag cactctgacg gatagtatta tcgataaacg
     3241 ccggatgaca catcacctcc agcgaacgat caccccgatg gcttgcgtca tccagcactt
     3301 gcaggaacag tgcctcgcta atctcttcac cataaaacgc gctactgaat ccctgagaac
     3361 tgcgcagatt agccggtaaa tcaccagcgt tagatagcgg ctgacggtcg atgcgcaacg
     3421 caatcccctc ttcagccgca aacctggcaa caatcgggaa aatctgcggg aacatatgca
     3481 catgatgatg gctatcaaga tgcgtaggtt tgcgtccaaa tagctcaatg aaacgcaaat
     3541 actgactggc aagctcctga gtaatttctt ccagcggtaa agcatcttct tctgccaact
     3601 gccagatcca ttttcccagc acaccatcgc gggttaaccc cggcatagct gtcaggggct
     3661 tgcccatagt aaggacaaag tgcatcccta tggccagact cggttcatca cgactcaact
     3721 gcaccgcatg gtcaatagcc tgcccattca ccagcgccgt cgttgatgtg acaatcccat
     3781 tgcgacaggc ctcgataatg ccgtagttct gacctttgct taagccaaaa tcatcggcat
     3841 taacaatcag taagcgttcc ataatcagcc tcggttaatg tgctttttta agctctgcga
     3901 tgcagtcggc aaaatttggc agccatttct cgtgcgccag aatcatctcg cgtgccagca
     3961 gctcagcatc gcgatcggaa tgcaccaacg gactaaggtt tagcgccagt aacacatcgt
     4021 taaattctcc gctaagtgcg gcgttgctgg cagcaatctc gaagccttta atggtgtgaa
     4081 tcagccccat cactttatca tcgaaatgcg taatgcgcgg atgtggcgtc gcgccatcgc
     4141 gccccagctt acaggtcatt tctaccgccc agtctgccgg aatattatca atatgcccat
     4201 gatgcgggat attaacgtaa tgttcagctt gcttgtcgtt gtagatagcg ttgatcactt
     4261 cgcacgctgc atcagagtaa taagccccac cgcgctgttc cagttctttc ggcttaactt
     4321 tcaactccgg atttttatac agctcaaaaa gttgtttctc gactttctgt actacctgtg
     4381 ctcgtgcgcc gcctttgtag tattcgccca tttcaatagc cagcatctct ttctgcttga
     4441 agtaatacag cagataagaa catggcagca gatttaacga acgaattaag ccctcactaa
     4501 atggcagatc gaaaatattt tttacagagg acgcttttaa ctgccctgac gccacaccat
     4561 caagcaattc ggcaaagcgc gacttgccat ttaccagcac atccttaatg aacaccatat
     4621 ggttgaggcc gaacagatcg atagataaat catcgctgtc tttcagcatc agaacatcgc
     4681 gaataaacat cttcatgccg atcggaatat tacacacgcc gataaagcgt ttaaatccgg
     4741 tatgacgata aacggcttca gtgaccattc ccgccgggtt agtgaagtta atcacccatg
     4801 cattcggaca aagttcttcg acatctttta cgatgtcaaa aatcaccgga atggtacgca
     4861 gacctttaaa cagaccgccc gcgccgttgg tttcctgacc aagataacca tgacttaatg
     4921 gaatacgttc atccagttcg cgcgccggta attggcctac gcgcagttgg gtagtaacga
     4981 aatcagcatc tttcaatgct tcgcggcgat ccagcgtttt ataaagcttc atcgggacgc
     5041 cagcgttatc aatcatccgt tggcagagtt caaaaatgat atccagtttc tctttaccat
     5101 cttcgacatc caccagccat aattcgctga ccggcaattc gtgataacgc ttaataaatc
     5161 cttccagtaa ctccggggta tagctgctcc cgccaccaat agtgacgact tttaatttct
     5221 ggctcataat ttctcccttc agtacagaat actgatatct ggcatatctg cccccccgga
     5281 cataaataat ccagcaacag gacagatatg tgaattgtca ggtataacga cttactgatt
     5341 aaattcagtc aatttcttac gatagctctt aggcgtaaag gatgttaatt tcttaaacgt
     5401 tttaataaac aaactcgggc tactataacc ggcctcaaac gcaatatccg tcacggaata
     5461 gttggtcatt tccagttgtt ttttggcaaa attaatacgg atctcattaa taatctgcat
     5521 tggtgttttg ccataatatc gttgagtcgc tcgcgtcaaa tattcctgtg atttggctga
     5581 caacgtcacc atattctcca gcgccgattc actaaactgc tctttatcat gcatcttttc
     5641 taccgtactt ttcagccact gcggtacatc atcaatcacc tgttcttcgc ggtaatgacg
     5701 taaacggtta atgacataaa aagtaaccat ctcaacaaac tcttccagcc ctgtttcgcg
     5761 gaaattcaat gaagaaatca ctgtttccac ataggtgaga aaagcgttat tggtccggta
     5821 tacctgcgaa gcgacaaagc aataaggcaa caacggcagg taatgctgct caaaaaagcg
     5881 tttactgatc ccaacgttca atatacgcgt ggcaccaaac tcataaaaac tttgatggtg
     5941 cgaacctaac ggaataaaaa caaaatcgcc ccgttccagt aacacgcgct taccgttaat
     6001 ctcctggaaa taacgcccgg ttaataccag agtaaattca taatagtcgt gctgatgcag
     6061 cccgctgata ctctcagttt tgttatagat aaacacatgg aagtttttgc cattaaacaa
     6121 ctgctgttct cgggcagtgg caatttccgg cgcgttaatc actggctgca tcatcgactc
     6181 cttatgcctt cagtttttca tgaagctcaa ttaattcagt aatcagttca cgcgcaagca
     6241 tggacgtcat taaatgatcc tgagcgtgga ccagcaccag actcactttc atcttacctt
     6301 cgcccgcatc gccttcaatc agtttcgtct gtaccagatg cgcttcattc aatgccatcc
     6361 gtgactgatc catcatggct tttgctgcag caaaatcgcc ctgcttcgcc tgtttcagtg
     6421 ccgcatacgc caggctgcgc gcttgtccgg agttgatgat aagccccatc accacttctt
     6481 ccagctcttc agcttctgtt tgcgtatcag gaatattatc gagatccatc atacatcgtt
     6541 cctctctctt cttaccggca agattacccg taccggcatc gattaaaatt tcagggcgtt
     6601 agcgatatct tcttcgctct cttctttatc aatcgcattt tgtgctttgt tagccaccac
     6661 aacaaagggc agataaatta aggttgcgat gccaaggttg aagagtgcga ccagcaatgc
     6721 ggcgacgcta ccgttggtgt taaagaaggc tcccagaccg gttggcatgg tccacggtgc
     6781 aatattggtc accggaggaa tgatgcccat gtagtacgct gcgagggtga ttgctgccag
     6841 aatcggttgt accagtacaa acgggataaa catcaccggg ttcatgataa ttggcagacc
     6901 aaacagaatc ggttcgttaa tctggaagat gccggacggc agcgccagtt ttgccacctg
     6961 acgataatca gcacggcgag aggcgataaa gatagccagg atcaggccta aagtcgcacc
     7021 actgccccca aggaaaataa aggagtccag caccggcttg gcccagatat ggaaggtctt
     7081 accggctgcc agcgccgctt cgacggaacc atattgctga taggtcgcga tattttccag
     7141 cgcccacggc gtcataatgc cgttgtccag tgcggtcagc gccagcgcgc catgaatacc
     7201 gaagaaccag agcagtggaa caaagatcac ataggcccag cccaccacgc tacccaacga
     7261 tgccagtggg gttgagatgg tatccataat gatctgatgg aagttggtgc cccaggtatt
     7321 caacgcccag gcaataatcc ccatcacgga aagaataata aagccgggaa ttaatgccga
     7381 gaaggaacgc gatactgaag caggtacgct gtcgggcagt ttaatgaccc aattgcggcg
     7441 gacaataaag gtaaacattt ctgccaccac caggccaata ataatcccgg agatgatatt
     7501 cgccccacct aaccagtttg cgccaaccgc ataggcctca ccgacactat atggggtgac
     7561 ggtcataaat gctgcaacgg ataacaaccc cgccgccagc gcatcgactt tacgctcttc
     7621 tgccagcgcc atgccaataa agaacggtgc cattaaagac attatcccta atgttccgtt
     7681 atatacgttg ccgccaatac ctttcaggcc attaagtgtt tcaatggttg aggcgtcgag
     7741 gcgaatacct aaggaataaa aaaacgaccc ctccccaaag cttagaaaaa cgttgttaat
     7801 taatacaaac atggcccccg caagggttaa cggcattaag cgaataaagc catttttgat
     7861 tgcattaacg tgtggctgct ttcctatttt aactgcaaaa gggaggagta ccttttcaag
     7921 cgatgcaata acattactca tagaaaaata cccttaaaaa ccgcaattta aatattgcgg
     7981 tattgattta tgaaataact ctttgacggg aaaatttaaa ataaattaat ttgctgcggc
     8041 ttttttaatc gctgcaaccg cagccttaag cacgcctaaa ccatcgactt tgccataaag
     8101 cagcgagtca attacttcaa ccggtttgtt gggtaacaaa cgctggattt cgggcaacat
     8161 ataagcaatc tgcggcccta ataacacgac atcggcattc tgaccttttt caccagccag
     8221 tgtttccgga aatgcttcaa taatgaccgg aacttcatat ttttctgcct gtgcgcgcat
     8281 ttttgatacc agtaaagagg tagacatgcc cgcagaacaa aacagataaa tgtgtttctt
     8341 ttccataaaa ctgccctcat cgacgattat ctgtcagcca gacactccgc aagccttaac
     8401 ctgcttccat gctctgggta acttgcgaaa ccaacatgac tttttttgtg gctgaaagga
     8461 gtatacggta tagacctgag caatagtatt tctttgacct tataaattgt ttctccttga
     8521 cctgtggtta tgaccctctt cacatttcgg gcaaataatt cgcgaagata attaagaact
     8581 caggccaaaa aaaaccggcg caatggccgg tttccgttgt tactcaagct ttcagacgaa
     8641 ttgattactt cgcagcctgt ggatcagtgt cgtattcagc acaggtctga taaccggagt
     8701 tgatgacatg tccggtatca tctaacgcga caaagtaggt ttctgcttta ccatcacgtt
     8761 gacccaggat gtaggtctgg caagtaccgc gagcatggat catgctcact tcagacgaag
     8821 gtttacccgc aatttgtgca acctgcgccc ggctcatgcc ttttttgacg tctttcacca
     8881 caggctgtac aaactggtct ttggtacgat cataagccgt acaacccgcc agcatggtta
     8941 ataccgccgc tgcactcaga attcctgcca tattcttgtt catattccgt cctcttgttt
     9001 atcagcgtgt tagataagcc tggaatacat tgggcgcttt ttcaagcccg tgaacgaaac
     9061 ggctccgctt tcagaggatt cctgtatgac gttttaacca ccattcagcc cgctgtcgct
     9121 tgtcgtttca gtagcaacgg gttagcttta aggaagtttt gtcttttctg tctggagggg
     9181 ttcaatgaca ttgcaacaac aaataataaa ggcgctgggc gcaaaaccgc agattaatgc
     9241 tgaagaggaa attcgtcgta gtgtcgattt tctgaaaagc tacctgcaaa cttatccgtt
     9301 cattaaatca ctggtgctcg ggatcagcgg cggtcaggac tccacgcttg ccggaaagct
     9361 gtgccagatg gcgattaatg agctgcgcca ggaaactggc aacgaatcac tgcaatttat
     9421 tgccgtacgc ctgccctatg gtgttcaggc tgacgaacaa gattgccagg atgccattgc
     9481 ctttattcaa ccggatcgcg tattaaccgt taatatcaag ggcgcggtat tggctagcga
     9541 gcaggcattg cgggaagcag gcattgaact gagcgatttt gtccgtggca atgaaaaagc
     9601 gcgtgagcgg atgaaagcac aatatagcat tgcgggtatg accagcggtg tcgtggtggg
     9661 caccgatcat gcagcagaag ccattaccgg attcttcact aaatatggtg acggcggtac
     9721 ggatattaat ccgctgtatc gtctcaacaa acgccagggt aaacagttgc tgacggcatt
     9781 aggttgcccg gaacaccttt ataagaaagc cccaacggcc gatctggaag atgatcgccc
     9841 ttctctgcca gatgaagtgg cactcggcgt gacctatgac aatatcgacg actatctgga
     9901 agggaaaaac gtacctgaac aggtcgccag aacaatagag aactggtatc tgaaaaccga
     9961 acataaacgc cgtccgccaa ttaccgtttt cgatgatttc tggaaaaagt aataatttgc
    10021 acataatggt ttgtgcgaaa aagagtaatt tgttcacgcc ggatgcggcg tgaacgcctt
    10081 attcgaccta caaaactatg caaattcaat atattgcagg agtcgaatag gcctgacagg
    10141 cgtagcacgt cagacggtgt aacctttgtc atcgacccgc ctctttttta atcgcttccc
    10201 gcctgttaca ctggatagat aaccagcatt cggagtcaac agtggtacgg cgtttaactt
    10261 ctccgcggct cgaatttgaa gctgcggcaa tttatgaata tcccgaacat ttacgttcat
    10321 tccttaatga cttacccacc cgaccagggg tgtatctgtt tcatggcgaa agtgacacca
    10381 tgccgctcta tatcggcaaa agcgttaata tccgcagtcg ggtgctttct catttacgca
    10441 ccccggatga agccgccatg ctacggcaat cccggcgaat tagctggatc tgcaccgcag
    10501 gcgaaatcgg cgctctgctc cttgaagcgc gattaatcaa agaacaacag ccgctgttta
    10561 ataaacggtt gcgccgcaat cgccagctct gtgccctgca attaaatgaa aagcgcgtcg
    10621 atgtggtgta tgccaaagag gtggattttt cacgagcccc caacctgttt ggcctgtttg
    10681 ccaataggcg cgcagctttg caagcattgc agagcatcgc tgatgaacaa aaactttgtt
    10741 atggcctgct gggactggaa ccgttaagtc gcggtcgtgc atgttttcgt tcagcgctaa
    10801 aacgttgcgc cggagcatgc tgcggtaaag agagccatga ggaacatgcg ctacgcttgc
    10861 gccagtctct ggagcgtttg cgggtggtgt gttggccttg gcaaggggcg gtggcgctga
    10921 aagaacagca cccggaaatg actcaatatc atattattca aaactggctg tggctggggg
    10981 cggttaattc gctggaagac gcgacaacgt taattcggac acccgccggg tttgatcacg
    11041 acggttataa aattctttgt aagccgctgc tttccggtaa ctatgaaatt actgaacttg
    11101 atccggcgaa tgaccagcga gccagttgat ttcactgaac aacagtttac cttgcggttg
    11161 taaaagccgc agggtgtgcc tgccatcgaa ccagcacgcg ccctggtcgg tagtgagcag
    11221 tttgtcgccc agttgccacg ccccgttaat cacaaacact accccgccgc gtgagccaaa
    11281 ggtagtaaac gtacgttcgg caattctgac tttcgcctta cagacatcca ggcgcgtcat
    11341 aatattgaaa tccatcgaca tctgccctgc cgtcagtttc gcttttacca cctggtccgc
    11401 cgcaaaggca aaaggctgta acggttttaa ggtatggtta aagtggtctg cgctttcaag
    11461 gaacatctcg ccgccttcca gcaacgtcac tatcctttcc ataccgggaa acaaagaaaa
    11521 ctccccattc gccgcgatgg atgcaatgct ggcacgccag taaaaatcac gttttgccgg
    11581 tgggaaagtg caaatttcgc gcgtttcacc ggcagcattt cgccacagat tcaccgacat
    11641 tttacgcata tcaaagtatt ccatactcgc tcccggcccg ttcttttcat tgttatcgtt
    11701 caggtaaccg tggatggtta tcggcaactc gggtgattta cgttagtggt gatcaggctt
    11761 tttttacaac tgtttgagaa gagaaaagaa aaccgccgat cctgtccacc gcattactgc
    11821 aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaaagattat tcagcagttg
    11881 caggcatttt accttttgcc gctggacgtt ctgtcagacg cttctcaaaa ttagcattaa
    11941 attgcttttt ctgttccggc gtcaggatgt tgtaaatttt gttctgggtt tccatgtgcg
    12001 ccagcatgtt agctttgcgc tgttcttcca tttttgcgat ctgcgcttca gcttttgctt
    12061 tatcgaaggt atcgctggca acgatgtcat gcattgcgcg gcgttcttcc agcggcggac
    12121 gtttcatctg gtcacgctgg cctttcatga tttcgcggat ctgctgtttc tgcgcgtcgg
    12181 tcaggttcag gtctttgaac atcatgtcct gatgcggacc gaacttgcct ttgtggtgca
    12241 taatcggctt cgcgtcagcc ggtgctgcgg tagtggtgtc tgcggcatgg gccaggttag
    12301 ccgcgccaag agccagggta gaggcaacaa acagtgcagt taatttacgc atattctata
    12361 tccttccttt cagttattta ttacggcttt cttaagtagc gtgccgtgtt gacgagatta
    12421 actttactga ctttagcgtc aattaatcag agcaacggta aaacaatgaa agtgtaaaaa
    12481 acacttttgc gccaattatg gagaaaaaaa gaaaatttga tggagagtga tgagagaata
    12541 ttacaacacg atgattttgc agagattatg aagaactata ccggatgact ggtgataaat
    12601 aaagcaaata accaggatta atctgtatta atttataaga aagcaactta atacccgcag
    12661 agtgatttct gcgggtaagt attagcttat tttttcgagc attaatcccg cgcgtaatcc
    12721 caacgctacc aacggattag ggaataacac atactctaca tcatgggtta cggtaaaacg
    12781 ttcctctccg tcctgcgcca gcaatgttcc tttctcaaac ggcataaaat tcagcgtgtc
    12841 acttgccata tgcatttcga aggacggcga gtggcgagta atttgcgaaa ccacccgata
    12901 acggagcggc ggtgttctca cgataccgac actctcacca gatagcagcg cagcaattgc
    12961 gctggcagtt actgcaaact ggcgaagatc gttttgccca aagggcaacg ctttgccaag
    13021 ttccagcgta caggccagcg cgccaaaatg tctggcgctg aaatgggtaa acgtaccacc
    13081 aggttcctga tggaacacca gcgcctccag ccccgccgca cccagccacg tcagaaattt
    13141 ctcgtcccag ggaatgtcgc gttgcggtaa tacaccgaac tgcggatgca aggagccacg
    13201 aattgcggta tgtagatcaa ggtgccagcg cacagattct ttgccctggt cataaaaatc
    13261 ttccaggcac tgttccagtt cgcgcgcccg acaggtttct ccgctttcag caaatagctg
    13321 ccaacgaccg ccaaacattc gattcatatc gctatggcaa taacgtttcc cttgcttcag
    13381 cgcaggagga ttcccgagga tcaccagcaa ccgccaacgt aacgggatct cgccgtgaga
    13441 tatcgcgcca agcaacgcgt ccagcatctc cacaggtgcc gtctcattac cgtgtattcc
    13501 cgctgaaatc accagtgcgc cttgcggtgg cgttaatggc gtcagttcca gcacaccatc
    13561 gcccagccag cgccagcgaa cgccgttgat ttctcgctcg gtgataaccg gttttttacc
    13621 cgttaaggtc agagcaagaa aattatccat tgccccctcc ctcgcgctgg aacggataaa
    13681 ccgaaccgag attcagtaat tggctcaata catccagtgc ttcccgccct tcgcgcagca
    13741 attgcgggtc ggccagatcg gcagcagtaa ggcgatcgcg gtagtaacga tccacccagt
    13801 cattgagcgc attaaacagc gtatcgttca tcatcaccgc cggattcacc gcccggcgtt
    13861 cttcttctgt caataccacc cgcaaccgca ggcacgccgg gccgccgcca ttcgccatgc
    13921 tttcacgtaa atcaaagact tttagttctc taatcgggtt gtcagcggca aggagttcat
    13981 tgagataacc ccatactccg gcgtgttccc gacactcctg aggcagcacc aacatcatgg
    14041 aaccatcatc gcggctcaac agttggctgt taaacagata ggtagacacc gcatccgaca
    14101 cggaaacctg agttgccgga acttctatcg ccataaaacc gttcacccgc gcacgcaggt
    14161 ttgccagtaa ctgtgactgg cgagcgaacg cctgttggtg gcaaaacagc acctggcggt
    14221 tactcacggc aatcacgtca ttatgaaaaa cgccctggtc gataacgtcc gggttttgct
    14281 gggcgaaaat cacctgttgg ggattcacct gattcagcct tgccaccgcc tcgctggctt
    14341 cgcgagtctg tcgcgccgga taacgggaag gccgggtatc attgccttct tctcgcccgt
    14401 agacaaaaag ttgcataccc ggctcaccgt aatgaccgcc gagacgattg tggtttgccg
    14461 ccccctcatc accgagcaac gctacctgcg gcaacgccga atgaacgcta aatttctctt
    14521 cgtcgttaaa aatcgctttt aaaagtgatt cggtgacgtg cgcttccagc gagcggtgaa
    14581 atttattgtt caggttggca acggtgagat gcactttgcc atccagcgta tcggcagatg
    14641 gcgcgatcgt tgccgcattg gctacccaca ttggcgaagc ggaactgacg ctggaaagcc
    14701 agtgcggtgc ctggcgtgca actttttcca gtacctgctc atcgctacca ctgaatccca
    14761 actgacgcag caccggaata aacggacgct cgtgcggcgg gatcacggcc tgggggaatc
    14821 ccgcatcggc aagggctttc attttcagta agccctgttt tgccgcctgt cgcgggttag
    14881 acacctgaaa acggtgacgg gtagaggctt cattaccaaa cgacaggcca gcgtaatgat
    14941 gcgtcagccc taccagcccg tcgaaattaa cttcccaggc gttcatcgca ccacctcatc
    15001 ggaaaaatcc agcccggggt taagcgttgc gggcaaagtt aacgagtccg actccaggct
    15061 cgccataggc catgcgcagt aatctgcggc ataccaggcg ctggggcgat ggttaccgga
    15121 tgcaccaatg ccgccgaatg gcgcggtact ggcagcacca gtaagcggtt tgttccagtt
    15181 aacaatcccc gctcgcgcct ccagcaacag ttgatcgaat ttttcccgct cgggggaaac
    15241 cagaccgcaa gagagtccga agcgagtgtt attcgccatt agaatcgctt cctcgaaaga
    15301 atcataacgc cagacgcgca ataacggtcc gaacacctct tcatctggta cgccagcaac
    15361 gcctgtcatt tcaatgatcc ccggcgtcag caacgatgtc tctgattgta ataagcgcgg
    15421 cgcaagcagg gttcgtccgc ccatcgcttc cagttgctgc caggcagtaa ccacctgctg
    15481 tgcggcctgt tcagaaatca gcccgccaat aaacggctgc ggttcgtcat cccagttgcc
    15541 cggcgttaat cgctggctga cggcaaccag acgagcaaga aacgcatcgc cctgcgctcc
    15601 gcttttgagc aataaacggc gggcgcaggt gcagcgttgc ccggctgtga caaacgccga
    15661 ctgaatggtc agatggacag ccgcgtcgat atccgccacc tcatcgataa ttagcggatt
    15721 attaccgccc atctcaaggg cgagaatttt ctccggctga ccggagagct ggcgatgcag
    15781 ctggtagccg gtattggcgc taccggtaaa cagcaaaccg tcgagatcct ccagcgcact
    15841 cagcgcctga cccgtttcac gcccgccctg caccaggttc agtacgcccg gcggcaagcc
    15901 agcctgctgc cataaacgca ttaccgcttc gccactccac ggtgtcagtt cgctgggttt
    15961 aaagataatg gtgttacctg ccagcaatgc cggaacgata tgtccgttcg gcaaatgacc
    16021 agggaaatta tacggcccaa acaccgccag cacgccgtgc gggcgatgtc gcaggctcgc
    16081 cgcaccgtcc ggcatttcac tacgctgctc gccggtacga acgtgatacg ccttaattga
    16141 tatcgcgatt ttattgatca tcgccgtcac ttcggttgcc gcttcccagc gcggcttacc
    16201 cgtttctctg gcaataatcg cggttaattc gcctttattc ctttccagca atccggcaaa
    16261 gcgttcgaca acgacctgac gttcagccaa tgagagccgc gcccagcgcg gaaacgccgc
    16321 acgggctgcc cgacaagcct gttcgacctg agcggcatcg gcatcattgc cttgccataa
    16381 cacctcgccc gataccggat tacgcttcac acgcgatgcg ccctggcccg ttatccagtc
    16441 accgttaatc cataaagtca tgctgttttc tcctctgcgc acaggcgcac cagacgaacg
    16501 cgatcccctg cgtggcattt gagggcatcc agttgtgcgg cggttaaaat caaacgctcg
    16561 gttgccggat cggtacgcgc cagcaccacg cggaaatggt gataattttc attggcgacc
    16621 aggcaggccg gaaaatcgcc ctgcgcaggc tgcccttctg ccacttccac cagccgactt
    16681 ttacgaatgg cgcgcacgcg gtcgatgtca cactcaagcg ttggcccgcc gtcgaagatg
    16741 tcgatatagt tgcggtaacg aaaaccttct ttctccagca ccgcgcgggc aggcgcggtt
    16801 tgcggatgta cctgaccgat gacgtcctgg gcttcctggg ataaaaagtg ggtatagatc
    16861 ggatgtttcg gcatcagttc tgcaataaat gccttttgcc cggtgccgca gagaaaatcg
    16921 gcgcggctaa aatccatcga aaagaagcgt ttaccgaggc tttgccagaa cggtgaatag
    16981 ccgtgttcgt caatcacccc gcgcatttca gcaaccactt tgtcattaaa cttgtcgcga
    17041 aaagccgcca taaacataaa gcgcgatttc gacagcaaat agccgttgcc ctctttgcgc
    17101 cagtccgggt cgagaaacag cgtgcacagc tcgctgctgc cggtgtgatc gttactgaga
    17161 aacagcgtcg gcaatgcgtt atagacattc agctcttttg aggcgtgaac caacgtgccg
    17221 acgcgatagt tgtaccaggg atcgttcagc ccaaccgcca cctcaatggc acaaatcccc
    17281 gccacggtgc ctgtctcgct atcttccagc acgaacacat agccctgctc acttttgggc
    17341 agttcgcctt gccaggtttt gattgccctt tcgatacgcg ccgaaagcgt ggcttcattg
    17401 gcgggaagcg acgtcaggcc gccgcccgtt ttgctggcaa gctgcatcag cgccgagaca
    17461 tctgatcgct caacgggacg gatgaccatc atgatgaacc tcggctaaca aagtgttcgc
    17521 aagcagctgc aaagcgatcc agtccggtcg tcacctcttc ttcgctgaca ttgagcgcag
    17581 gcgcaaaacg caccacgttg ccacccgcaa tcagtaccat cacgcctgct ttcgccgctt
    17641 cctgagagat ctgtttcgct tgcccggcgt aatcggcatt cagtacacag ccaatcagca
    17701 aacctaagcc gcgaacttca ctgaacaaac catagcggtg attaatagta ttaagacgct
    17761 caacaaacca gtcgtgacgc tgtttaacgc cattaagcat ctctggtgtg ttgatgagct
    17821 ccagcacttt gcctgccacc gccgaggcca gcggattacc gccataggtg gtgccatgag
    17881 tgccaacggt catcacgcgg gcgcactctt cggttgccaa caacgcaccg accgggaaac
    17941 cgccgcccag cgctttggcg gtagttaaca gatcaggcgt cacgccgtag tgcatatagg
    18001 catacagttc cccggtgcgc ccgacgccgg tttgtacttc atcaaaaatc aacagcgcat
    18061 tgtggcggtt acacaattca cgcagacctt gtaaaaacgc gttgctggct ggcaccacac
    18121 cgccttcccc ctggatgggt tcgacaatca ccgcacaggt agagtcgtca atcagcgcgc
    18181 tggcagagtt aatatcgtta tatgcagcat gacgaatatc cgccggcagt ggcgcaaaat
    18241 cctgtgaata ggctggctgc ccacccgcac tgacagtaaa cagcgtgcga ccatgaaacg
    18301 catttttgaa cgccacgatg ccgctcttat ggctgccgta gcggtcgtga gcgaatttac
    18361 gcgccagttt tagcgccgct tcgttggctt ccgcaccgga gttacaaaag aagacgcgat
    18421 cggcaaacgt ggcgtcgatc aattttttcg ccagtcgcag taccggctcg ttggtgtaac
    18481 cgttgccggt atgccagaac ttactcgcct gttcgttcag cgcttcacgc agttccggat
    18541 gcgcatggcc cagcgcgttc accgcaatgc cacccgcgaa gtcgatatac tctttcccct
    18601 gctgatccca caagcgcgaa ccttcgccac gtaccggtat aaagggtgcc ggagcgtaaa
    18661 caggtatcat ccattcatca aagttttcac gcgtaattgg ctgagacata gcgacctcta
    18721 cagtaaataa ttcgttattt atatgttaac aataagtaat gtttgcgctg taaatgtaga
    18781 ttgcagggtt cgtgccagcc agtgataaaa gtgcataaac ggcggaggct aactggaaat
    18841 caaggagtta taaccaaacc atatgcattt aaagtgcata taaagtgaat acgtttgcga
    18901 tgtgggtgaa taaaaagaat aaaaaacgca atgttatgca gaagtaaaat ataattctgg
    18961 aattgtgatc attgacgaaa tttactggaa attactgcgc cattctgacg cagcgcgcac
    19021 caaaagcggg cattttttgc gccatcgttg acatcattaa caaccatcga tcaaatcact
    19081 taacaacagg cggtaagcaa cgcgaaattc tgctaccatc cacgcactct ttatctgaat
    19141 aaatggcagc gactatgaaa tttgtctctt ttaatatcaa cggcctgcgc gccagacctc
    19201 accagcttga agccatcgtc gaaaagcacc aaccggatgt gattggcctg caggagacaa
    19261 aagttcatga cgatatgttt ccgctcgaag aggtggcgaa gctcggctac aacgtgtttt
    19321 atcacgggca gaaaggccat tatggcgtgg cgctgctgac caaagagacg ccgattgccg
    19381 tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg gcggattatt atggcggaaa
    19441 tcccctcact gctgggtaat gtcaccgtga tcaacggtta cttcccgcag ggtgaaagcc
    19501 gcgaccatcc gataaaattc ccggcaaaag cgcagtttta tcagaatctg caaaactacc
    19561 tggaaaccga actcaaacgt gataatccgg tactgattat gggcgatatg aatatcagcc
    19621 ctacagatct ggatatcggc attggcgaag aaaaccgtaa gcgctggctg cgtaccggta
    19681 aatgctcttt cctgccggaa gagcgcgaat ggatggacag gctgatgagc tgggggttgg
    19741 tcgatacctt ccgccatgct aatccgcaaa cagcagatcg tttctcatgg tttgattacc
    19801 gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga cctgctgctc gccagccaac
    19861 cgctggcaga atgttgcgta gaaaccggca tcgactatga aattcgcagc atggaaaaac
    19921 cgtccgatca cgcccccgtc tgggcgacct tccgccgcta atttagcagc tctcctggct
    19981 caaactgggt caggagaatt aaccttgaga aaaatcaaca aactgtcagt aatgatttgt
    20041 tgcctgccgt cctttgttat accgtcactg cgtttttagt tgtctgacca cttctctatt
    20101 atcaagtttg atataggaaa ctccacgatg aacgctgagc gtaactttct gtttgcctgt
    20161 cttatttttg cgctggtcat ttacgctatc cacgctttcg gtttattcga tctgctcacc
    20221 gatttacccc acttacagac actcattcgc cagagcggat tgttcggtta tagcctctat
    20281 attctgttat tcatcattgc caccctcttt ctgttaccag gaagcatact ggtgatcgct
    20341 ggaggaatag tttttggccc gctcttaggg acactactct cattaattgc cgcaacgctg
    20401 gcctcctcgt gctcattcct gctggcgcgc tggctgggac gtgatttact gctgaaatac
    20461 gtgggccata gccatacctt ccaggccatt gaaaaaggca ttgcgcgtaa cggtattgat
    20521 tttcttattc tgacccgctt aatcccgttg tttccttaca atattcaaaa ttacgcttac
    20581 ggattaacca ccatcgcctt ctggccttat acccttattt cggcactcac gaccctgccc
    20641 ggtattgtta tttataccgt gatggcaagc gatctcgcca atgaaggcat tacgctgcgc
    20701 tttattttac aactctgtct ggcgggcctg gcgctgttta ttctcgtcca gctcgcaaaa
    20761 ctctacgccc gacacaaaca tgtggatctg tctgcttcgc gccgcagccc acttactcac
    20821 ccaaaaaatg aaggatagaa cgatgtcgca acattattca gtgtcatgga aaaaaggact
    20881 ggctgcactc tgcttactgg ctgttgccgg gcttagcggc tgcgatcaaa aagagaatgc
    20941 ggcggcaaaa gtggaatacg acggactttc gaacagccaa ccgctgcgcg tcgatgccaa
    21001 taaccatacg gtaaccatgc tggtgcaaat taatggtcgt ttcctcaccg acgacactcg
    21061 tcacggtatt gtgtttaaag atggctccaa cggacataaa tcgctgttta tggcttatgc
    21121 gaccccgaaa gcattttatg aagccctgaa agaggcaggt ggtacgccgg gcgaaaacat
    21181 gacgatggat aataaagaaa cgactcatgt cacaggcagc aaactggata tttcggtcaa
    21241 ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg attgttgaca gtaatggcaa
    21301 gaaactggac atgcgctttg gcggtaattt aacggcagca gaagagaaga aaacgggttg
    21361 cctggtgtgt ctggatagct gcccggtcgg catcgtcagc aatgcaacat acacttatgg
    21421 tgcggtggaa aaacgcggcg aagtgaaatt caaaggcaat gcctcagttc tcccggcgga
    21481 taacacgctg gcaacggtta cctttaaaat caccgaataa agccaggata aaggatgatg
    21541 aagatacaat cgcgtaaaat ctggtactac cgtataaccc tcatcatcct gttgttcgct
    21601 atgctgctcg catgggcgct gcttcccggc gtccatgagt ttatcaatcg cagcgttgcg
    21661 gcgtttgccg ccgtggacca acagggtata gaacgcttta ttcagtctta cggcgcactg
    21721 gcggcagttg tctcgttctt gttgatgatt ttacaggcca ttgctgcacc gctacctgcg
    21781 tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt tctggggcgg cttgctgtcg
    21841 tggaccagtt cgatggccgg cgcggcgctg tgctttttta tcgccagagt gatgggccgc
    21901 gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca gtatggacgg ctttttcact
    21961 cgctacggca aacacaccat cctggtctgt cggttattgc cttttgtccc tttcgatcca
    22021 atcagctatg ctgccggtct ggcttcaata cgttttcgct cgttttttat cgccaccggg
    22081 cttggtcaat taccggcgac tattgtttat tcctgggcgg gcagcatgtt aacaggcggt
    22141 actttctggt ttgtcaccgg actgtttatt ctgtttgccc tgaccgtggt gatttttatg
    22201 gcgaagaaaa tatggcttga gcgccagaag aggaatgcct gatgggttta ccgccgctta
    22261 gcaaaatttc tttcatttta cgtccgcagg cgtggctgca tcgtcgccat tacggcgagg
    22321 tgctaagccc gattcgctgg tgggggcgga tcccgtttat cttttatttg gtgtcaatgt
    22381 ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc ggtagtgcga tcgcttgtca
    22441 gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt ggatatcacc agtatgaaag
    22501 tcgccgagcg taccggcagc accgataaac tgctggcagt ggctgactgg cggcaaagcc
    22561 cgctcttcag cgatgaagaa cggctggcgc tggagtacgc cgaagccgca agcgtaacgc
    22621 cgccaacggt cgatgatgcc ctgcgcaccc gactggctgc gcattttgac gctcaggcgc
    22681 tcaccgaact gacggcattg atcggcctgc aaaacctgtc ggctcgtttt aattctgcca
    22741 tggacattcc cgctcagggg ctgtgccgta ttccggaaaa acgttcttaa ggagagaaga
    22801 tgcgccattg tgggtggttg ctgggattgt tatcgctgtt ttctctggca acacatgcca
    22861 gtgactggca ggaaattaaa aatgaggcca aagggcaaac cgtctggttt aacgcctggg
    22921 gcggcgatac cgcaattaac cgctatctcg actgggttag cggcgagatg aaaacccatt
    22981 acgctataaa cctgaagatt gttcgtctgg cggatgccgc agacgcggtg aagcgcattc
    23041 agaccgaagc tgctgccgga cgtaaaacgg gcggctcggt ggatctgctc tgggtgaacg
    23101 gcgaaaactt ccgcacctta aaagaggcca atttattaca aacgggctgg gcggagactc
    23161 tgcccaaccg gcgctatgtc gacacacagc tgccggtgcg ggaagatttt tcagtgccta
    23221 cagaaggggc tgaatcgccc tggggcggcg cacaactgac gtttatcgcc cgccgcgatg
    23281 ttacgccaca gccaccacaa acgccgcaag ccttactgga gtttgctaaa gccaatcccg
    23341 gcacggttac ctatccgcgc ccaccggact ttaccggcac ggcgtttctt gaacagttgc
    23401 tgattatgct gacgcccgat cccgccgcgt taaaagttgc cccggataaa gccactttcg
    23461 cccgcgtcac tgcgcccttg tggcaatatc ttgacgcgct acatccctat ttatggcgca
    23521 aaggaaagga tttcccgcct tcgcccgcgc ggatggatgc tctgctgaaa gccggcacat
    23581 tgcgcctgtc gctgaccttt aaccctgctc atgcccagca aaaaatcgcc agcggcgatt
    23641 tgcctgcaag cagttacagt tttggtttta accaggggat gattggcaac gtgcatttcg
    23701 tcaccattcc tgctaacgca aatgccagtg ctgcggcgaa ggtggttgcc aacttcctgc
    23761 tctcacccaa tgcgcaactg cgtaaagcgg atcccgctgt ctggggcgat ccttctgttc
    23821 tcgatccgca aaaactgcct gatgggcagc gcgaaacatt gcaatcaaga atgccgcaag
    23881 atctgccgcc ggtactggct gaaccgcacg caggttgggt aaatgcgctg gaacaagaat
    23941 ggctacaccg ttacggtacg cattaatctt tttgctgtgg gcgatggtgg cggtgattta
    24001 tgcaccactg atcccggcag ctctcacgct gatatcgcct gctttgtcgt tgacacactg
    24061 gcaggcgtta tttgccgatc cacagttacc gcaggcatta ctggcaacgc tggtgtcgac
    24121 aaccatcgcg gcggtcgggg cgttgttgat tgccctgctg gtgattgtgg cgctgtggcc
    24181 ttggccgaaa tggcagcgta tgtgtgcccg tctgccgtgg ctgctcgcca ttccccatgt
    24241 ggcttttgcc accagcgccc ttctgctctt tgctgacgga gggctgcttt atgactattt
    24301 cccgtatttc actccgctaa tggacaaatt gggtattggg ctgggcctca cccttgcagt
    24361 gaaagaaagc gcatttctgc tgtggatctt agcggcagta ttgagcgaaa aacggctgtt
    24421 gcagcaggtc attgtgctgg attcgctggg ctacagccgc tggcaatgcc tgaactggct
    24481 gctcttgccc tccgtcgcac ctgcactggc aatggcgatg ttggcgattg ttgcctggtc
    24541 gctttcggtc gtggatgtgg cgattattct tgggccaggt aatccaccga cgctggcagt
    24601 aattagctgg cagtggttaa ctcagggcga cgcggatcaa caaacgaaag gcgcactcgc
    24661 cagtctgctg ctgatgctgt tactcgccgc ctacgttttg ctgagctatc tgctatggcg
    24721 cagctggcgg cgcactattc cccgcgtaga tggcattcgc aagcctgcca cacctttatt
    24781 accaggcact acgctggcga gttttttacc cttaaccggt gtgctgtgtg tggttctgct
    24841 ggcgatcctc gcggatcagt cgacgatcaa tagtgaagca ctcatcaaca gcctgacaat
    24901 ggggctggtg gcgacattca tcgctttgct cctgttactg ctgtggctgg aatgggggcc
    24961 acagcgtcgc cagttgtggc tctggttgcc cactctattg cccgctctgc cactggtggc
    25021 gggccagtac acgctggcgc tatggctgaa cctggatgga agctggacgg cggtggtctg
    25081 ggggcatctg ctgtgggtaa tgccgtggat gctgtttatc ctgcaacccg cctggcagcg
    25141 cattgattca cggttaattt tgattgcgca aacactgagc tggtcgcggg ccaaaatctt
    25201 cttttacgtg aaatgtccgc ttatgctacg ccctacgctg attgccttcg cggtgggatt
    25261 ttcagtcagt attgcgcagt atatgccgac gctgtggctg ggcgcggggc gttttccgac
    25321 gctcaccact gaggcggtgg cattaagcag cggcggcagc aatggtattt tcgccgccca
    25381 ggctttatgg caactgctat taccgcttat tatttttgcc ctgaccgcgt tagtcgcaaa
    25441 atgggtaggt tatgtcagac aaggactccg ctaatgctct gcgtgaaaaa tgtttcgcta
    25501 cgtttaccag aaagccgctt gctgacaaac gttaacttta cggtggataa aggtgacatt
    25561 gtcacgttaa tggggccgtc tggctgtgga aaatccactc tgttttcatg gatgattggt
    25621 gcactggctg gacagttttc ttgtacaggt gagctatggc tcaatgagca acggattgac
    25681 atgctaccca ccgcacagcg tcagattggc attcttttcc aggatgcact gttatttgac
    25741 cagttcagcg tcgggcaaaa tttactgctg gcgctaccgt cgacgctcaa ggggactgcc
    25801 aggcgcaatg ccgtgaaaga cgcgcttgat cgagcgggac tggctgaaac ttatcaccag
    25861 gaccctgcga cattatcagg cggtcagcga gcgcgggtgg ccctgctgcg cgcccttctc
    25921 gctcagccaa aagcgttact cctggatgag ccattcagcc gtcttgatgt ggctctgcgc
    25981 gataattttc gccagtgggt gttcagcgaa gttcgcgaac tggcgatccc cgtcgttcag
    26041 gtaacgcacg atctccagga tgttcctgct gatagttctg ttctggatat ggagcagtgg
    26101 tcagaaaatt acaacaaact gcgataacgc aaagtttttc tcaatgcgtc agttcagaat
    26161 ggcgcactca aaactacaat gtcgggattt tcgatgaaac gtgtttctca aatgaccgcg
    26221 ctggcaatgg ctttagggct ggcttgcgct tcttcgtggg ccgctgaact ggcgaagcct
    26281 cttacacttg accagcttca acaacaaaat ggcaaagcga tagatactcg ccccagcgcg
    26341 ttttataacg gctggccaca aaccttaaat ggcccttctg gtcatgaacc tgccgcctta
    26401 aacctctctg ccagctggct cgacaaaatg agcaccgaac agctcaacgc gtggatcaag
    26461 caacataacc tgaaagccga tgccccggtg gcgctgtacg gtaatgacaa agatgtcgac
    26521 gccgtcaaaa cgcgactgca aaaagcaggt tttacgcata tctccattct gagtgacgcg
    26581 ctaagcgaac cttcccgtct acaaaaactg ccgcactttg agcagcaggt ttatccgcaa
    26641 tggctgcacg acctgcaaca aggtaaagag gttacggcga aacctgccgg tgactggaaa
    26701 gtcattgaag cggcctgggg cgctcctaag ctttacctta tcagccatat tcccggtgct
    26761 gactacatcg ataccaacga agtggaaagc gaaccgctgt ggaacaaagt ttctgatgaa
    26821 caactaaaag cgatgctggc aaaacacggc attcgccatg acaccacggt cattctgtac
    26881 gggcgtgacg tatacgctgg agcgcgtgtg gcgcaaatta tgctctacgc aggcgtgaaa
    26941 gatgtgcgtc tgctggatgg cggctggcaa acctggtccg acgcgggact gcctgttgag
    27001 cgaggaacgc caccgaaagt gaaagcggaa ccggatttcg gcgtgaagat cccggcacaa
    27061 ccgcagttga tgctcgatat ggaacaggcc cgtggactgc tgcatcgcca ggatgcatcg
    27121 ctggtgagca ttcgttcgtg gccagaattt atcggtacga ccagcggtta cagctatatt
    27181 aaaccaaaag gtgaaatagc cggagcacgt tggggacacg ctggtagcga ctcgacgcat
    27241 atggaagatt ttcataaccc ggatggcacc atgcgcagcg ccgatgatat taccgctatg
    27301 tggaaagcgt ggaatatcaa accagagcag caagtttcat tctactgcgg caccggctgg
    27361 cgcgcgtccg aaacctttat gtacgcacgc gccatgggtt ggaagaatgt ttccgtgtat
    27421 gacggcggct ggtacgaatg gagcagcgat ccaaaaaatc cggtagcaac cggtgaacgc
    27481 ggcccggaca gtagcaaata acattgcaat tactgacgct ggtgcgactt cagcgtcaga
    27541 tacccactcc acacccgcgt aaaggtcgtc atccagcaca acgcaccgaa gatccacgca
    27601 aaccacgcaa accatgcagg gaataaacat cccagcacaa acagtaagat cgtttctgtg
    27661 ccttcggtta acccgcccag ataataaaac gatttatgcg cataaccggg attatcaatc
    27721 tgatgtttcg ccgccagcgc agcaaaagcg agaaaactgc tgcctgtgcc gataaacgca
    27781 aacagcagcc agccgcctgc cagggcgttt tgctgcggtg cggcgaggat aaacccaaaa
    27841 ggtaccagcg cgtaaaaaag aaaatcgaga gaaatatcaa gaaagccgcc cgcgtcggta
    27901 agccctctgc gccgcgccag cgcaccgtcc agaccatcaa gcagcctgtt caacaaaatg
    27961 acgacaagcg ccgccagata ccagcccagc gccagaaaag gcagtgccag cacgccgatg
    28021 gcaaatccca ctaatgttaa accatctggc gtaatgcccg gtttatcaag aacccgcacg
    28081 cactgatgca acaacggttt aatccgggga tgaagatggc ggtctagcac aggcactcct
    28141 taaatataaa gcctttctga ttgagcaaca gtgcggatat tatggcattt ttcgcttatc
    28201 tgcccgtgtg taatttatga aaatgattga agttgttgcc gccatcattg aacgtgatgg
    28261 caaaatttta ctcgcgcaac gccccgccca gagcgatcag gcgggattat gggagtttgc
    28321 cggtggtaaa gtcgagccgg atgaaagcca gcggcaggcg ctggtgcgtg agttacgcga
    28381 aaaactgggc atcgaagcaa ctgtgggtga gtatgttacc agccatcagc gagaagtttc
    28441 gggccggatt atccatcttc atgcctggca cgtacccgac ttccacggga cgttacaggc
    28501 acatgaacat caggcgctgg tctggtgctc acctgaagag gcgctgcaat atccgctggc
    28561 ccctgctgac attccattat tagaggcgtt tatggcttta cgcgccgcca gaccagcgga
    28621 ttagtgctaa gggttttgtc atcccgctgg cattgcagca gtattccctc ggctttaatt
    28681 accgcgcctt cagaataatt tttatcctga taaacgcagc actgagtaca gggctgcgct
    28741 gactgcccgc ctgaactgaa cacttctggc ggtacgttta cctccacgtc cggacgataa
    28801 tgcgggttag ccagtgcaat taatggaaat gctaatacta cggcgaacaa tgctcgactc
    28861 acagggaact ccttaacgtt attgtctctg ctactgataa cggtagccgg gtggcaaaac
    28921 tttagcgtct gagttatcgc atttggttat gagattactc tcgttattaa tttgctttcc
    28981 tgggtcattt ttttcttgct taccgtcaca ttcttgatgg tatagtcgaa aactgcaaaa
    29041 gcacatcaca taaacaacat aagcacaatc gtattaatat ataagggttt tatatctatg
    29101 gatcagacat attctctgga gtcattcctc aaccatgtcc aaaagcgcga cccgaatcaa
    29161 accgagttcg cgcaagccgt tcgtgaagta atgaccacac tctggccttt tcttgaacaa
    29221 aatccaaaat atcgccagat gtcattactg gagcgtctgg ttgaaccgga gcgcgtgatc
    29281 cagtttcgcg tggtatgggt tgatgatcgc aatcaggtac aggtcaaccg tgcatggcgt
    29341 gtgcagttca gctctgccat cggcccgtac aaaggcggta tgcgcttcca tccgtcagtt
    29401 aacctctcca ttcttaaatt cctcggcttc gaacaaacct tcaaaaatgc cctgactacc
    29461 ctgccgatgg gcggtggtaa aggcggcagc gatttcgatc cgaaaggaaa aagcgaaggt
    29521 gaagtgatgc gtttttgcca ggcgctgatg actgaactgt atcgccacct gggcgcggat
    29581 accgacgttc cggcaggtga tatcggggtt ggtggtcgtg aagtcggctt tatggcgggg
    29641 atgatgaaaa agctctccaa caataccgcc tgcgtcttca ccggtaaggg cctttcattt
    29701 ggcggcagtc ttattcgccc ggaagctacc ggctacggtc tggtttattt cacagaagca
    29761 atgctaaaac gccacggtat gggttttgaa gggatgcgcg tttccgtttc tggctccggc
    29821 aacgtcgccc agtacgctat cgaaaaagcg atggaatttg gtgctcgtgt gatcactgcg
    29881 tcagactcca gcggcactgt agttgatgaa agcggattca cgaaagagaa actggcacgt
    29941 cttatcgaaa tcaaagccag ccgcgatggt cgagtggcag attatgccaa agaatttggt
    30001 ctggtctatc tcgaacgcca acagccgtgg tctgtaccgg ttgatatcgc cctgccttgc
    30061 gccacccaga acgaactgga tgttgacgcc gcgcatcagc ttatcgccaa tggcgttaaa
    30121 gccgtcgccg aaggggcaaa tatgccgacc accatcgaag cgactgaact gttccagcag
    30181 gcaggcgtac tgtttgcacc gggtaaagcg gctaatgctg gcggtgtagc aacgtcaggc
    30241 cttgaaatgg cgcaaaacgc tgcgcgcctg ggctggaaag ccgagaaagt tgacgcacgt
    30301 ttgcatcaca tcatgctgga tatccaccat gcctgtgttg agcatggtgg tgaaggtgag
    30361 caaaccaact acgtgcaggg cgcgaacatt gccggttttg tgaaggttgc cgatgcgatg
    30421 ctggcgcagg gtgtgattta agttgtaaat gcctgatggc gctacgctta tcaggcctac
    30481 aaatgggcac aattcattgc agttacgctc taatgtaggc cgggcaagcg cagcgccccc
    30541 ggcaaaattt caggcgttta tgagtattta acggatgatg ctccccacgg aacatttctt
    30601 atgggccaac ggcatttctt actgtagtgc tcccaaaact gcttgtcgta acgataacac
    30661 gcttcaagtt cagcatccgt taactttctg cgatagcagc agatatgcca gtaaagaaac
    30721 cccattagac tatttttttg ataatcttct tcgctttcga acaactcgtg cgcctttcga
    30781 gaagcaagca ttatataatg ccaggccagt tcttcttcaa ttgtcccgtt ttgaaaagct
    30841 gtgcttgata tcgaggtcat ccatgataat tccgccgccc atattagctt cgccgaggat
    30901 ttaccggagc gattattagc gcaatcagag atatagtctg agggaaaaac agcaaattta
    30961 ttcaacaagg cgataacctg ctctggggct tcctccatgt ttgctttaaa gatattggct
    31021 ccatggtcgc cagaaagaaa atgctccatt aaggcacaat aactttcgct atcttcgata
    31081 ccccattgat cctctaaaga ctcgcgtctt ttacttatga tatcgatcga gtcaaaggga
    31141 agcacatgat attggaaggt atctttgcca ggttcaggct ttcgcggcca gaactccagc
    31201 gtttcagacc attgcttatg atagaaccga taaggtgcga tcaattgtag cgcctgtaac
    31261 ttctcgatac tgagcggctc aataccttta gcctgataat aatgcagttg ttcttttttt
    31321 gctttaaaac cggcccgaac aataagcccc atcataatta atagataaag aaaagagcat
    31381 cccgcggtaa tcaggcctct ttcattcaaa ccgttggatg ttatcgctgc gaacacaaac
    31441 attacagcaa caacacatgt taaataaaac ccccacttac aaagcagcat ggccttattt
    31501 tctttaatca tccgttcaaa attactatta aatatttccc agccattaaa agaatacttc
    31561 tcgctcccag gatggttttg taataaaact tttttcatca cattccctgt tattacatac
    31621 tttagaggcg ataattttat ccatgcaaaa aaaatatcca acgaattttc ttgatccgct
    31681 ggatatttca gaatatgact cgaatagcac gaaagattca ctcgcttacg ctatcgcccc
    31741 gcttccgact tcatctgctg gcggactttt tttcgcacta cgtttacgcg gtgcagcctt
    31801 tttcttatca gcactgccac cactgcccgg agccacaatg ccgcgaaact gccgcaccgg
    31861 cgtacgtttg gcttgatcaa taagctgata tagcgtcccc accagcggct gcataaagtc
    31921 ctgatagcga cactgctttt cgctgatttg cgtcagcacc gattcccagt gcgcggtcat
    31981 gtccggtcgc gtcgccattt ctggcagcga atggaataac gcttttccgg cgtcggtaga
    32041 gtggatatag cgcccttttt tggtcagaaa accacgcttg aacagcagtt caataatccc
    32101 ggcacgggtt gcttctgtcc ccagaccatc ggtcgcacga aggatctttt tcagatcttt
    32161 gtcctgcaca aagcgcgcga tcccggtcat cgccgaaagc agtgttgcat cggtaaaatg
    32221 gcgcggcggc tgggtttgcc gctctaccac ttcacctttt tcacacagca actcatcgcc
    32281 tttcgccacc acaggcaatg gcgtaccgtc gttttcttca tcgcgctctt tgctgcctaa
    32341 cagcgtgcgc cagcctgctt cagcaagaaa acgggcttta gcgacaaatt tgcctttggc
    32401 aatgtccagt tcgataacac acttgcggaa caccgcatcc gggcagaact gcatcaggta
    32461 ttgacgggca atcaggttat agaccttcgc ttcgttctcc gtcaggttga tcgcagaact
    32521 ccgtgcggtc gggatgatgg cgtggtgcgc atcgaccttt ttgtcatccc aacagcggtt
    32581 gcgtatatct ggatctacca ctgactgcgg caacagatcc ggtgcatgaa cgctgatggc
    32641 attcatcacc gcgtggcgtc cggcaaaatg ttcttctggc aaatagcgac aatcggaacg
    32701 cggataagtg attagcttgt gcgtttcgta cagcttctgg cagatatcaa gcacgttctg
    32761 cgcgctcaga ccgaagcgtt ttgccgcttc aatctgcaac gccgaaagcg aaaacggcag
    32821 cggcgcggat tctgattccc gtttatcgtt atagctggtg acaatagccg gttgaccact
    32881 aatgcggtta accacatgct ccgccagtgg acgatgtaac aagcgccctt cttcatcctg
    32941 gtacggttca cacgcttcgc tcggttgcca gatagcggta aaccgctcat cggcaggtgt
    33001 cacgatatgc gctttgactt caaagaagtc tttcgccacg aagttttcaa tctcttcatc
    33061 gcggcgcacc accagcccga gcacgggtgt ctgcacgcgt cccacggaaa gtacgccctg
    33121 ataaccggca ttgcgaccga gaatggtata cgcacgggtc atattgatgc cgtacagcca
    33181 gtcggcacgc gctcgcgcca gcgcagaaac gcacagcggt acaaactcac tgttggaacg
    33241 aagacggtcg atcgcccgct caaccgcctg cgggttcagg tcgtttatca agcaacgctg
    33301 cacctgctgg cgcttttccg gtgccagttg cagatagtcc agcacttcat ccaccagcaa
    33361 ttgcccttca cgatccgggt ccccggcgtg aacgatttcg ctggcttcat gcaggaagcg
    33421 tttgatgacg ttaagttgtt tggtcacgga gggtcggggc tgtaattgcc acttttccgg
    33481 gacaatcggc aaatccgcaa gattccagcg cgcatagcgg ctgtcgtagg cgtctggctg
    33541 cgcctgctca agcaggtgac cgatacacca ggtcaccacc tgaccattac cgcactcgat
    33601 aaagccatcg cctttacggt gcggtttggg caggacatca gcaatggcgc gcgccagact
    33661 cggtttttcg gcaataaaca accgcattga attaatggat ctcaaccatg gcacgaccgc
    33721 cgcgcgctgg caccagttcg ccaatcgccg tcagttcaat gccaaactcg gcggctgtag
    33781 ttttaacctc attttctgct tccggcatga ccgccagcag caaaccgcca gaagtttgcg
    33841 gatcgcacag cagatcgcgc acttcacgcg gaatttcacc catcagatga ccgtagctgg
    33901 caaagttacg ttcggtgccg ccaggcactg cgcccaactt aatgtactct tcaacccccg
    33961 gcaattttgg gatcgcgtca tagtcaacgc gcgcctgcac acctgcgccc tgacacattt
    34021 cgctcaaatg gcccagcaga ccaaaaccag taacgtcggt catcgctttt acgccttcga
    34081 tgttggcaaa ggacgcacct gcgatgttca tccggcacat tacttccgtc gccagtccct
    34141 gatgttctgg tttcaacagt gattttttct cagccgtggt aagaacgccg atccccagcg
    34201 gtttcgtcag gaacagtttg catccggctt gtgcggtact gtttttcttc acccgctcgg
    34261 tcggtacgat ccccgttacc gccagaccaa aaatcggctc cggcgcatcg atggagtgac
    34321 cgccagccag cgcaataccc gcctgacggc atgcatagcg tccaccttcg gttacttcgc
    34381 gggcaatttc tggggaaagt ttgttaatcg gccagccgag gatcgcaatc gccataatcg
    34441 gtttgccccc catcgcgaag atatcgctga tggcgttagt cgccgcaatg cggccaaaat
    34501 cgaaaggatt atcaacgatc ggcataaaga agtcggtggt actgataacg ctggtgccat
    34561 tgcccagatc gtacaccgcc gcatcgtcgc gggtctcatt acccacaagc aaattcggat
    34621 caacaaactt cgcctgctca ctgtgcagga tggtttccaa cacttttggg gaaattttac
    34681 agccgcaacc agctccgtgg ctgtattggg tcaaacgaat cgagttctcg ctcatggaca
    34741 tctcctgtca atgcaatccg ggtatggtaa ccctcattcc gtgaagtgat aagtgagagt
    34801 gtctgaattc ctgcgccttt gctcacaatc cagacagttt cgcgacaatt atcagaaata
    34861 agttacaaac ggcgtcgggt ccgggacgtt aatcgacgta gatgctttca actgcggcgt
    34921 gccgaggtag agaaaaccga caattttatc ctgctcacgg caaccgaatg cttcacgcac
    34981 taccggactt tcagttaatg cgccactgcg ccagatgccg ccaaatcctt gggcaacggc
    35041 tgccatttgc atcgccatga ccgcgcatcc ggcagacatt tcctgttccc atcgcgggac
    35101 tttatgattt tcttcgcatt tcgccaccac cgtgatgatg agcggtgcgc ggaacggcgc
    35161 attacgggct ttgtcgatag ctttgtcatc actaccggca gcaatcgccc cctgttccag
    35221 tacggcgctg aaacgctcgc gcccttcccc ttcaatcaca aaaaaatgcc acggttgcat
    35281 ggacttatgg tccggcgcac gcatacccgc acgcaggatg ttttgcagtt gttcacccgt
    35341 tggcgcgggt tcagccaggc gggaggcgct acggcgattg atcaatagtt cgagtgcatc
    35401 catttgatta actcctgtcg tgatatttat tcacaaaatt aacacgagag tggattttgt
    35461 tacagcacag tccgcaattc ctgctgacaa gtgccggttg ggtcattacg ataaccacat
    35521 ctattgcgcc tgtgacaggt gtgaccttaa gttgggagaa tacatgcgaa ccctttggcg
    35581 atttattgcc ggatttttta aatggacgtg gcgtctgctg aatttcgtcc gtgaaatggt
    35641 acttaacctg ttctttattt tcctcgtact ggttggtgtg gggatctgga tgcaggtcag
    35701 tggtggtgat tcgaaagaaa cggccagtcg tggcgcactg ctgctggaca tttctggtgt
    35761 gatcgtcgat aaacccgaca gttctcagcg gtttagtaaa ttaagccgcc agctgcttgg
    35821 tgccagttcc gatcgtctgc aggaaaactc actgtttgat atcgtcaaca ccattcgcca
    35881 ggcgaaggac gaccgcaata tcaccggtat cgtgatggat ctgaaaaact tcgcaggcgg
    35941 cgaccagccg tctatgcagt acatcggcaa agccctgaaa gagtttcgtg acagcgggaa
    36001 accggtttat gccgttggcg agaactacag ccaggggcaa tattatctcg ccagtttcgc
    36061 caataaaatt tggctgtcac cgcaaggcgt ggttgatctg cacggttttg ctaccaacgg
    36121 tctgtactac aaatcgttgc tggataagct gaaagtttcc acccatgtgt tccgcgtggg
    36181 tacgtataaa tctgccgttg aaccgtttat tcgtgatgat atgtcaccgg cagcccgcga
    36241 agctgacagc cgctggattg gtgagctgtg gcaaaactat ctgaatactg ttgccgctaa
    36301 ccggcagatc cctgctcagc aggtattccc tggcgcgcaa gggttgcttg agggtttaac
    36361 caaaaccggt ggcgataccg cgaaatatgc actggaaaac aagctggtcg atgcactggc
    36421 atcgagtgcc gaaatcgaaa aagctctgac caaagagttc ggctggagta agactgataa
    36481 aaattatcgc gccatcagtt attacgatta cgcattgaaa acgccggcag ataccggtga
    36541 cagcatcggt gtcgtcttcg ctaatggcgc aattatggat ggcgaggaaa ctcaggggaa
    36601 tgttggcggt gataccactg cggcacaaat ccgcgacgct cgccttgacc cgaaagtgaa
    36661 agcgattgtc ctgcgtgtta atagcccagg cggcagcgtt accgcgtctg aagtgattcg
    36721 cgctgaactg gcagcagccc gggcagcggg taagcctgtg gttgtatcga tgggcggcat
    36781 ggcggcatct ggtggttact ggatttccac gccagctaat tacattgtgg ctaaccccag
    36841 caccctgacc ggttctatcg gtatcttcgg cgtgatcacc accgtagaaa atagtctgga
    36901 ttcgattggt gttcatactg atggtgtctc aacttcaccg ctggcggatg tttctatcac
    36961 cagggcactg ccgccggaag cgcagcagat gatgcaatta agcattgaga atggctataa
    37021 acgctttatc acgctggttg ctgatgcgcg tcattcgacg ccggagcaaa ttgataaaat
    37081 cgcccagggc cacgtctgga ccggtcagga tgcaaaagct aacgggctgg tcgatagtct
    37141 cggggatttc gatgatgcgg ttgccaaagc agcagagctg gcaaaagtga aacagtggca
    37201 tctggaatac tacgttgatg aaccgacctt cttcgacaaa gtgatggaca acatgtctgg
    37261 ttctgtccgg gcaatgttgc cagatgcgtt ccaggccatg ttacctgcac cgcttgcctc
    37321 ggtagcctct accgttaaaa gtgaaagcga caagctggcc gcgtttaacg acccacaaaa
    37381 ccgttatgcg ttttgcctga cctgcgccaa cgtgcgttaa gtcttgtact gagtggccga
    37441 cagatcgtcg gccacattat tttttacctc gacgaatcct cttcccgctg tctcgaccca
    37501 tatttcctta tccgcatagt atcaggtgcg ctcctcctgc ctcacgtata tacttttgct
    37561 ctttcgatat cattcatatc aatatcatgc aaaagaaatc aatttacgtc gcctacacgg
    37621 gcgggaccat cgggatgcag cgttccgagc agggttatat tccggtgtca ggtcatctac
    37681 aacgccaact ggcgctgatg ccggaattcc atcgcccgga gatgccagat ttcaccattc
    37741 atgaatatac gccgttgatg gattcttctg atatgacgcc ggaagactgg cagcatattg
    37801 ctgaagatat taaagcgcac tatgacgact atgatggttt tgtcattcta cacggtaccg
    37861 acacgatggc gtataccgcc tctgcgctgt cgttcatgct cgagaatctc ggtaaaccgg
    37921 tcattgtgac agggtcacaa atcccgctgg ctgagttacg ctctgacgga caaattaatc
    37981 tgctgaatgc gttgtacgtt gcggcgaatt atccgatcaa cgaagtaacg ctctttttca
    38041 ataaccgatt gtatcgcggc aaccgcacta ccaaagccca tgccgatggt tttgatgcgt
    38101 ttgcctctcc aaaccttcct ccgttactgg aagctggtat ccatattcgt cgtttgaata
    38161 cgccacccgc cccgcacggt gaaggggaat taatcgttca tccaatcacg ccacaaccaa
    38221 ttggcgtagt gacgatttat ccagggattt ctgctgacgt cgtgcgcaat tttctgcgcc
    38281 aaccggtgaa agcattgatt ctgcgctcct atggcgtggg taatgcgcca caaaacaaag
    38341 ccttcctgca ggaattacaa gaagccagcg atcgcggtat tgtggtggtc aacctgacac
    38401 aatgtatgtc cggtaaagtg aatatgggtg gttatgccac cggtaacgcc ctcgcccatg
    38461 ccggcgttat tggcggtgca gatatgactg tagaagccac gctaaccaaa ctgcattacc
    38521 tgctgagcca ggaactggat actgaaacca ttcgcaaggc catgagccaa aacctgcgcg
    38581 gcgaactgac gccggatgat taaggagact gcaatgcccc atcgcgccct gttactggtc
    38641 gatttacaaa atgatttctg tgctggtggc gcgctcgccg tgccggaagg tgacagtacg
    38701 gtagatgtcg ctaaccgcct gattgactgg tgccagtcgc gcggtgaagc ggttatcgcc
    38761 agtcaggact ggcacccggc gaatcacagc agttttgcca gtcagcacgg tgtagagcct
    38821 tatacgccag gccaactcga cggtttgcca caaaccttct ggccagatca ctgtgtgcag
    38881 aacagtgaag gcgcacaatt acatccgtta ctgaaccaaa aagcgatcgc agcggtgttc
    38941 cataaaggcg aaaatccttt agttgacagt tacagtgcct tttttgataa cggccgtcgg
    39001 cagaaaacct ctctcgatga ctggttacgc gatcatgaaa tcgatgaatt gatcgttatg
    39061 ggcctggcta ctgactattg cgtgaagttt accgtgctgg acgcgttaca gttaggttat
    39121 aaggtaaacg tgattaccga tggttgtcgt ggcgtgaata tccagcccca ggacagtgcg
    39181 cacgcgttta tggagatgtc agcagctggg gcaacgctat atacgctggc agactgggaa
    39241 gagacgcagg ggtaatatta gttgaattca tctctggcct acatttcctc aatgacattg
    39301 taggccagat aatacttgtc agtgttgcat ttaacctact tgcagctaaa acacgacgtc
    39361 ctaacaagta tatttgccct cccttaataa caggcttaaa tactagataa ctcattcact
    39421 taaacatagc aataggcttt gctgagatac caaaatcttc ttttaactgc tgtttgctct
    39481 tcatgaccat ctgaccattt gtgtcaattg tcatatgctg tgcttcggta ttatggcggg
    39541 cttgccacag catcaccagt tgcaggcaat tctctttttg ttcctcggtc aacgcaacgc
    39601 catcaggcca tttccccagt tcaacggcgg tcgacaaacg ctggtatacc tcaggcgtca
    39661 tactgttgat aatgtcatca agattcataa tttttccgct cctgtggaat aagttgctga
    39721 atcgtttttt caaccgttga tttcttcgcc gttttcgcca tcagtgaagc gtaaagaagc
    39781 agagttaaca caataacgtt cgcctgttgg ttgcggcccg tcgggaaaga catgccccag
    39841 atgggcatca cagttaccgc aacgaatttc tatgcgctgc attccatgtg acaagtcttt
    39901 gatataacga atggattctt cactcaccgg ttcgtagaaa ctgggccagc cacagccgga
    39961 atcatacttg gtttcggaat gaaacagcgg ggcatcgcag atcaaacagt gatatacgcc
    40021 gtcacgcttg ttatgcagta aacaacccgt aaatggcggt tctgtcccat gattctgcgt
    40081 cacgtaaaac tgcatctcgg acaaattttt tttcagttct tctgccgaag gtttattagc
    40141 catttgctca catctcactt taatcgtgct cacattacgt gactgattct aacaaaacat
    40201 taacaccaac tggcaaaatt ttgtcctaaa cttgatctcg acgaaatggc tgcacctaaa
    40261 tcgtgatgaa aatcacattt ttatcgtaat tgccctttaa aattcggggc gccgacccca
    40321 tgtggtctca agcccaaagg aagagtgagg cgagtcagtc gcgtaatgct taggcacagg
    40381 attgatttgt cgcaatgatt gacacgattc cgcttgacgc tgcgtaaggt ttttgtaatt
    40441 ttacaggcaa ccttttattc actaacaaat agctggtgga atatatgact atcaaagtag
    40501 gtatcaacgg ttttggccgt atcggtcgca ttgttttccg tgctgctcag aaacgttctg
    40561 acatcgagat cgttgcaatc aacgacctgt tagacgctga ttacatggca tacatgctga
    40621 aatatgactc cactcacggc cgtttcgacg gtaccgttga agtgaaagac ggtcatctga
    40681 tcgttaacgg taaaaaaatc cgtgttaccg ctgaacgtga tccggctaac ctgaaatggg
    40741 acgaagttgg tgttgacgtt gtcgctgaag caactggtct gttcctgact gacgaaactg
    40801 ctcgtaaaca catcaccgct ggtgcgaaga aagtggttat gactggtccg tctaaagaca
    40861 acactccgat gttcgttaaa ggcgctaact tcgacaaata tgctggccag gacatcgttt
    40921 ccaacgcttc ctgcaccacc aactgcctgg ctccgctggc taaagttatc aacgataact
    40981 tcggcatcat cgaaggtctg atgaccaccg ttcacgctac taccgctact cagaaaaccg
    41041 ttgatggccc gtctcacaaa gactggcgcg gcggccgcgg cgcttcccag aacatcatcc
    41101 cgtcctctac cggtgctgct aaagctgtag gtaaagtact gccagaactg aatggcaaac
    41161 tgactggtat ggcgttccgc gttccgaccc cgaacgtatc cgtagttgac ctgaccgttc
    41221 gtctggaaaa agctgcaact tacgagcaga tcaaagctgc cgttaaagct gctgctgaag
    41281 gcgaaatgaa aggcgttctg ggctacaccg aagatgacgt agtatctacc gatttcaacg
    41341 gcgaagtttg cacttccgtg ttcgatgcta aagctggtat cgctctgaac gacaacttcg
    41401 tgaaactggt atcttggtac gacaacgaaa ccggttactc caacaaagtt ctggacctga
    41461 tcgctcacat ctccaaataa gttgagatga cactgtgatc taaaaagagc gacttcggtc
    41521 gctctttttt ttacctgata aaatgaagtt aaaggactgc gtcatgatta agaaaatttt
    41581 tgcccttccg gtcatcgaac aaatctcccc tgtcctctcc cgtcgtaaac tggatgaact
    41641 ggacctcatt gtggtcgatc atccccaggt aaaagcctct tttgcattac agggcgcaca
    41701 ccttctctcg tggaaacctg cgggtgaaga agaagttctg tggttgagca acaacacacc
    41761 gttcaaaaat ggcgtcgcta ttcgcggtgg cgtaccggtt tgctggccgt ggtttggtcc
    41821 ggcggcacaa caaggtctgc ctgcgcacgg ttttgcccgc aacctgccgt ggacgctgaa
    41881 atcacatcgt gaagatgctg atggcgtagc gctgactttt gaattgacgc aaagcgaaga
    41941 gacgaaaaaa ttctggccgc acgactttac gctgttagcg catttccgcg tgggtaaaac
    42001 ctgtgaaatc gatcttgaat cacatggcga atttgaaacc acctctgccc tgcataccta
    42061 ctttaacgtg ggtgatatcg ctaaggtaag cgtcagtggg ctgggcgatc gcttcattga
    42121 taaagtaaat gacgcgaaag aagatgtact gaccgacggt attcagacct tccctgaccg
    42181 taccgatcgc gtgtatctga atccgcaaga ttgcagcgtg attaatgatg aagcgctgaa
    42241 tcgtattatc gccgtaggcc accagcatca tctgaacgtt gtcggctgga acccgggacc
    42301 ggcgctttca gttagcatgg gcgacatgcc ggatgatggc tacaaaacat ttgtttgtgt
    42361 agaaacggct tacgcttcag aaacgcaaaa agtgaccaaa gagaaacctg cacatctggc
    42421 gcaatccatt cgcgttgcga aacgttaatt tgcgttaatg ttgtgtgccg ggtgcgatgc
    42481 atccggcacg tatcaacatc acaccatatc cagcgcagtt tttccttttg gtgccggata
    42541 cgctttatcc agcatggtta attccgctga cgaaagttcg acctcaagca cagccgcatt
    42601 ctgttggaca tgggcaatcg tggccgcttt tggaatcgcc atcacaccct gatggctgat
    42661 tacccacgcc aacaatactt gtgccgcgct gatattgtga gcatgtgcaa tttcgttgac
    42721 taccgcgttt tttaacagtc cattgcgcaa ccgcccggcc tgggctaatg gactgtaagc
    42781 catcaccggc atctgctgtt gctggcacca ggggagtaga tcgtactcaa tccctcgtga
    42841 accgagatgg taaagcacct gattagtggc acactgattt ccccccggca gctgccagag
    42901 ttcctgcata tcagcatgat caaggttaga aacgccccag cggcggattt ttccctgggc
    42961 gatcaatttt tccatcgctg ccacagtctc ttcaaaagcg aaactgccag accagtgtaa
    43021 taagtagaga tcgagataat cagtattgag acggcgtaaa ctggcttcgc atgcatttat
    43081 cgctttttgc ccgccagcat tccacggata gactttagag acgagaaaga cgttatcgcg
    43141 cagaccggtt aatgcttccc caaccacctt ttcggcaccg ccatcggcat acatttcggc
    43201 ggtatcaatg agggttaaac cgagctcaat gcccgcgcgt agtgcagcaa cttctgtttt
    43261 gcgctgactg gcatcttcgc ccatatacca tgttccctgc cctatggctg gcagtgagac
    43321 atcgccacta aattgaatca ttttttgttg cattgtttcc tcccaggtat tgcaccaccg
    43381 taatgcaaaa cagggcgtga cgccctgttt ttatgcacaa aatgccctgg aaagatgcat
    43441 tatcagaatt tgtaggtgat cccggtagaa atcaggccag tccaggattt atccaccatc
    43501 gggctgtcag taacttcatc agacagacgg gtatagcgcg cggtaccgta aacactccag
    43561 tcgccgagga agttgtagct ggcgctcagc tccaggtaag ggctccagct gtcgttcggg
    43621 ttatagccac gcagaccgct gcgagcggac tctttgcgcg atacgccata atagtattcg
    43681 ttctggtttt cgctgttcca ctgcacaccg atacccgggg tcacggtcag gccgccgttg
    43741 gtgtaacgat acaaccaggc catatcccag acgatgccgt tgctgttatc cagggtatcg
    43801 ccagccaggg tggtacgcag gtaaccgtac tgggtaaagt gagcataaga cagaccagcc
    43861 atcatggtgc tcttacggtc atccaggtga cgcatttggt gatcgccact gtctttcgct
    43921 ttgaagtaaa gcggcgacca ataagcggta attgaaagtt tgtccgttgc gtcattccac
    43981 aggtagtaac caccacctaa gccacggaac cagaagttat cgccttcata gttgattacc
    44041 ggtactgggt aaacatcggt atcgtaatct ttatatgggt gctcaacgac tcctacgcct
    44101 gcgcccaggg aaaatttacc ttcagcgtgc gctacgcctg cagacgttgc gataagcact
    44161 ccaagtgcca gaagtttgag tttggtcaca attaatcatt ccttaaacaa atgtttagcg
    44221 ggcgacaaag tttacccgtc aatacatcga aacccaacct ttttacgttt tcatttttta
    44281 aagtaactgt ttaattttcc tgacgcggat gacaccgcgc ttacagccaa atgaatttag
    44341 cgttactggc taccctggtc tttacattaa ttatgcaaaa tttatggatg agttgttgat
    44401 atgccattga aattaagaaa gccgtgcagg caagttttcc atttgccatc tacgcttaat
    44461 tttgaaggtg tatcaccggg cacgttgttc tcatcgtcga taaaatggca tgagagttgc
    44521 tgtgttttag caagagacgt cgttcagttt acctcttccg ggagcctcta ctattcatat
    44581 gaacggctct taacctgtgc taaaaaacga aaggacggca taccatgaat atattcgatc
    44641 actatcgcca gcgatatgaa gctgccaagg acgaagagtt cacactgcag gagtttctta
    44701 ccacttgtcg gcaagatcgc agtgcttatg ccaacgcggc tgagcggcta ttgatggcta
    44761 tcggtgagcc tgtcatggtc gatacagccc aggaacccag actttctcga ctcttttcta
    44821 accgggtcat tgcacgttat ccggcatttg aagagtttta cggcatggaa gacgcgattg
    44881 aacagattgt ctcttatctg aaacacgcgg ctcaggggct ggaagagaag aaacaaatcc
    44941 tgtatctgct ggggcctgtg ggtgggggta aatcatcgct tgctgagcga ctgaaatcat
    45001 taatgcagct cgtaccgatt tatgtattga gcgccaacgg tgagcgtagc ccggtcaacg
    45061 atcatccgtt ctgtcttttc aatccgcagg aagatgctca gattctggaa aaagagtatg
    45121 gcattcctcg ccgttatctc ggcaccatca tgtcgccatg ggcggcaaaa cgcctgcatg
    45181 aatttggtgg cgatatcact aagttccggg tagtgaaggt ctggccgtca attctgcaac
    45241 aaattgctat cgccaaaacg gaacccggtg atgagaacaa ccaggacatc tccgcgctgg
    45301 ttgggaaagt cgatattcgt aaactcgaac actacgcgca gaatgacccg gacgcctacg
    45361 gctattccgg tgcgctgtgc cgcgccaacc aagggatcat ggaatttgtc gagatgttta
    45421 aagcaccgat aaaagtgctg catccgctgt taaccgccac tcaggaaggt aactacaacg
    45481 ggacggaagg tatctccgcc ctgccgttca acgggattat tctcgcccac tcgaacgaat
    45541 ccgaatgggt cactttccgt aataacaaaa acaacgaagc cttcctcgac cgtgtttaca
    45601 tcgtgaaggt gccgtattgc ttacgcattt ccgaagagat caaaatctac gagaaattgc
    45661 ttaatcacag tgaattgact cacgccccct gcgcccctgg cacgctggaa acgctgtcac
    45721 gtttttccat tctttcgcgc cttaaagagc cagaaaactc cagcatttat tcaaagatgc
    45781 gggtttatga tggcgaaagt ctgaaagaca ccgatcccaa agccaagtcg tatcaggaat
    45841 atcgtgacta cgccggtgtc gatgaaggga tgaacggtct gtcgacgcgt tttgcgttta
    45901 agatcctctc ccgcgtgttc aacttcgatc atgtagaagt ggcggcaaac ccggtccatc
    45961 tgttctacgt cctggaacag cagatcgagc gcgagcagtt cccacaagag caggcagaac
    46021 gctatctgga gttcctgaaa ggttatctga tcccgaaata tgccgagttt atcggcaaag
    46081 agatccagac ggcctacctt gaatcctatt ccgaatatgg gcaaaacatt ttcgaccgtt
    46141 atgttaccta cgcggatttc tggattcagg atcaggagta tcgcgatccg gataccgggc
    46201 agctgtttga ccgcgagtct cttaacgccg agctggagaa aatcgagaaa ccggcgggga
    46261 tcagtaatcc aaaagatttc cgcaacgaga ttgttaactt cgtactgcgc gccagagcga
    46321 ataacagcgg acgcaatccg aactggacca gctatgaaaa actgcgcacg gttatcgaga
    46381 agaaaatgtt ctccaatacc gaggagctgt tgccggttat ctcgtttaac gccaaaacgt
    46441 caaccgacga gcagaagaaa cacgacgact ttgtcgaccg tatgatggaa aaaggctaca
    46501 cccgtaaaca ggtgcgttta ctgtgcgaat ggtatttgcg cgtacgtaaa tcgtcttaac
    46561 aaccctggcc cggtacacat gttaccgggc ctacaacgac agcgaaccgt gggcctgaga
    46621 agcggcaaca caggcgtagc atacagttgg caaatgtagt acggggggca tatgacctgg
    46681 tttattgacc ggcgtctgaa cggcaaaaac aaaagcatgg tgaatcgcca gcgtttttta
    46741 cgccgttata aagcgcaaat taaacagtcg atctccgagg ccattaataa gcgttcggtg
    46801 actgacgtcg acagcggcga atccgtatcc attcccacgg aagatattag cgaaccgata
    46861 tttcatcagg ggcgtggcgg tctgcgccac cgcgtgcatc cgggcaatga ccatttcgtc
    46921 cagaacgacc gaattgaacg tccccagggt ggcggcggag gttccggtag tggtcagggc
    46981 caggccagcc aggatggtga aggtcaggat gaatttgtct ttcagatttc gaaagatgag
    47041 tatcttgatt tgctctttga agatttggcc ttaccgaatc tgaaacaaaa ccaacaacgc
    47101 cagctgaccg aatataaaac gcatcgggcg ggttataccg ctaacggcgt tccggcaaat
    47161 atcagcgttg tgcgctcctt gcagaactca ctggcgcgac gcacagccat gacggcaggc
    47221 aagcggcggg aacttcatgc actggaagag aatttggcca tcatcagcaa cagtgaacct
    47281 gcgcagctgc tggaagagga acgtctgcgc aaagaaattg cagaattacg tgccaaaatt
    47341 gaacgcgtcc cttttattga caccttcgat ttacgttaca agaactacga gaagcggccc
    47401 gatccctcca gccaggcagt gatgttttgc ctgatggacg tttccggttc aatggatcaa
    47461 tccactaaag atatggctaa gcgtttttat attctgctgt atctgttcct cagcagaacg
    47521 tataagaacg tggaagtcgt atacatccgc catcataccc aggcgaaaga agtcgatgaa
    47581 catgagtttt tctactcgca ggaaacaggc ggcaccattg tttccagcgc cctgaaactg
    47641 atggatgagg tagtgaaaga gcgttataac ccggcacagt ggaatattta cgctgcacaa
    47701 gcatcggacg gcgataactg ggccgatgac tctccgcttt gccatgaaat cctggcgaaa
    47761 aaattattac ctgttgttcg ttattacagc tatatcgaaa ttacccgtcg tgcacatcag
    47821 acattgtggc gagaatatga gcatctgcaa tctactttcg acaactttgc gatgcagcac
    47881 atccgcgacc aggatgatat ttatccggtg ttccgtgaac tgtttcataa acaaaatgca
    47941 acagctaaag actaaaacta tcagccaggt cattatcgcc tggctgattt ttagcttact
    48001 gtaaattatc tcatttatta catacattag cttacaatcg ctttaaatat gacagcgtaa
    48061 cctttacata atttagttcc agaaaacaat cattcggaaa aatgattcag tcaacacgta
    48121 tttccatggg gttattcttt aaatattgtt tatcgttaac gaaaattgat cctggtcaaa
    48181 actatatatc tctgccatca ataaaatcca gcactcacat tgctctcctt tttatggttt
    48241 ctatgggtac acaaaaatta aaagctcaaa gcttttttat tttcagttta ttgctgacgt
    48301 taattttatt ttgcatcacc acattatata acgaaaacac aaatgtaaaa ctcatcccac
    48361 agatgaatta cctgatggtt gttgtggctt tgtttttcct taacgccgtc atttttcttt
    48421 tcatgttaat gaaatatttc actaacaaac aaattttacc aacactcatt ttaagccttg
    48481 catttttaag tggccttatc tatttagttg aaaccattgt aattatccat aaaccaatta
    48541 acggcagtac actgatccag acaaagtcga atgatgtttc tattttctat attttccgcc
    48601 aactcagttt tatttgttta acctcgctgg cactcttttg ttatggaaaa gacaacatcc
    48661 ttgacaacaa taagaaaaaa acgggaatcc tgttgctggc gctgattcct tttttagttt
    48721 ttccccttct ggcacacaat ctgagcagtt ataacgctga ctattctttg tatgtcgtcg
    48781 actactgtcc ggacaaccat actgcgacct ggggaatcaa ctatacaaaa atattggttt
    48841 gtctgtgggc atttttactg ttctttatta tcatgcgtac acgattagcc agcgaactat
    48901 ggccgttaat agcattatta tgtctggcat cgctatgctg caacttactt ctactgactc
    48961 tggatgagta taattacacc atctggtata tcagtcgcgg gattgaagtt tccagtaaac
    49021 tgtttgttgt gtcttttctg atttataaca tttttcagga actgcaactc tccagcaaac
    49081 tggcagttca tgatgtgctg accaatattt ataatcggcg ctactttttc aacagcgtag
    49141 agtcattatt gtcgcgacct gttgttaagg acttctgtgt catgctggtt gatattaatc
    49201 agttcaaacg catcaatgcc caatggggac atcgtgtggg tgataaagtg ctggtttcaa
    49261 ttgtcgatat tatccagcaa agcatccgcc ctgatgatat tttagcgcga ctggagggtg
    49321 aggtgtttgg cttgctattt accgaactca atagtgccca ggcaaaaatc attgcggaac
    49381 gtatgcgtaa aaatgtcgaa ctcctgaccg gctttagtaa cagatatgat gttcctgaac
    49441 aaatgaccat cagtattggc acggtttttt caacgggtga cacgcgtaat atctcgcttg
    49501 tcatgacgga agcagataaa gccttacgcg aagcgaaaag cgaggggggc aacaaagtga
    49561 ttattcatca tatttaagtg caaaaatatt cagagccatg ctttttgcgt ggcttttgca
    49621 tacaatttat tactattacc cctaaatttc ccctccccag cggcgcagag atgagtataa
    49681 ttagcgcccc tgtgccaggc cgcaatcgaa ctttatctgg ttttctcgtt tcactaaccg
    49741 aaggagtgcc atttatcatg aaattgcacc atagaatgct ccggcatttt atcgccgcaa
    49801 gtgtcattgt gctgacatct tccttcctta tttttgaact tgtcgccagc gacagagcaa
    49861 tgagtgccta tctgcgctat atcgtgcaga aagcagactc ctcctttctt tatgataagt
    49921 atcagaatca gagtattgcc gcgcatgtga tgcgcgctct cgctgctgag cagtcggaag
    49981 tgtcgccaga acagcggcgc gccatctgcg aggcttttga gtctgccaat aatacccatg
    50041 gcttaaacct gactgcccat aaatacccgg gcttacgcgg cacactacaa accgcatcca
    50101 ctgactgcga cacaattgtg gaagctgcag cactattacc cgcttttgat caggcagtgg
    50161 aaggcaaccg ccaccaggat gattacggtt caggtcttgg gatggccgaa gagaaatttc
    50221 actattatct cgatctcaat gaccgctatg tctattttta tgagccggtt aatgttgaat
    50281 actttgcgat gaataactgg tccttcctgc agtcaggaag tattggcatc gatcgcaaag
    50341 atattgaaaa ggtatttacc gggcgtaccg tattgtcgag catttaccag gatcagcgta
    50401 ctaaacagaa cgtgatgagt ttgctgacgc cggtatatgt cgcagggcag ctaaaaggga
    50461 ttgtgctgct ggatattaac aaaaacaatt tgcggaatat cttttacact catgaccgcc
    50521 ctctcctctg gcgttttctc aatgtcacgc taaccgatac cgattcgggg cgcgacatta
    50581 tcatcaacca gagcgaagat aatctgttcc agtacgtcag ttacgtccat gacttaccgg
    50641 gcggcattcg tgtctcgtta tccattgata ttctttactt tatcacgtct tcgtggaaaa
    50701 gcgttctgtt ctggattttg gcggcgttaa ttttgctgaa tatggtccgg atgcacttcc
    50761 gtttatacca aaatgtgtcg cgagaaaata ttagtgatgc gatgactgga ctgtataatc
    50821 gcaaaatttt aacccctgaa ctggagcagc ggttgcagaa actggtgcaa tccggttctt
    50881 cggtgatgtt tattgcaatt gacatggaca agttaaagca aataaatgac accctcggtc
    50941 atcaggaggg ggatttagcg attacgttat tagctcaggc gattaaacaa tcgattcgta
    51001 aaagtgatta tgccatccga ctcggtggcg atgaattctg catcattctt gtcgattcga
    51061 cgccgcaaat tgcagcacaa ctgcctgaac gtatcgaaaa acgtctgcaa catatcgcgc
    51121 cgcagaaaga aatcggcttc tcttccggta tttacgcgat gaaagaaaac gatacgttac
    51181 atgatgcgta taaagcttcc gatgagcgtt tatatgtcaa taagcagaac aaaaacagcc
    51241 gttcatgata accttctgtg gttgtttgct tgtaatctca ggagcgtgaa atgactgaaa
    51301 tggctaaagg aagcgtgacg catcagcggt taatcgcttt attatcacaa gaaggtgcta
    51361 acttccgcgt cgttacccat gaagcggtag gtaaatgtga agcggtgtca gaaattcgtg
    51421 gcaccgctct aggccagggt gctaaggcac tggtatgtaa agtcaaaggc aatggcgtaa
    51481 atcaacatgt tctggcgatc ctcgctgccg atcaacaggc cgatctgagc caactcgcca
    51541 gccatatagg cggattacgc gcttcgttgg ccagcccggc ggaagtcgat gaactcacag
    51601 gctgtgtctt cggcgcgatc ccccctttca gcttccatcc aaaactcaaa ctggttgccg
    51661 accctctact cttcgaacga tttgatgaaa tcgccttcaa tgcagggatg ctggataaat
    51721 ccgttatttt gaaaaccacg gattatttgc gcattgcgca accagaactg ctcaatttcc
    51781 gccgcactgc gtaactagcc tgtccgttca ataagcagaa cggacaaaac cagcacaaca
    51841 gcaatggcaa aaaacgatga cgtgataatc agtgtctcga cgaacatttg atcgttcata
    51901 gcatgccccc gggagatagc tgttttcacg ttattgttag cgtgcacaaa tggcagtttg
    51961 atgacagttc gcctgatttt ttatgcaaaa aacgtaaaga ttttctactt ccttcctgca
    52021 gcaagcgtaa agtaagcagg cttattattt tttggcaagg aaaccacgat gtttgatgtc
    52081 actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa caccactgtc
    52141 gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac cgttaagcac ctttttcccg
    52201 tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg cgttatggcc
    52261 cccatcgcca gcgggacgct accgccctca accttgattc actcgtttct gaactggaaa
    52321 tcgctggtgg caattgcagt aggcgttatt gtctcctggc tgggtgggcg cggcgtgacc
    52381 ttaatgggca gccagccgca actggtcgcc gggctgctgg taggcactgt tttaggcgtg
    52441 gcgctgtttc gcggcgtacc ggtcggcccg cttattgccg cgggtctggt ttcgctgatt
    52501 gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc aggcctttct
    52561 taaacatagt aataaaggcc gttgttgaat cataccccag cgtatgagca actttctgta
    52621 cagtatcgcc tttcactaat ccctgaagtg ccataatcag ctgtaactgc tgacgccact
    52681 ggcgaaaact caaccccgtc tcttttacta tcagtcgcgc aagattgcgt tcactcatcg
    52741 caaaaaagcc agcccattgc cctaatgacc cccactcgac gggccctttc gccatcatct
    52801 ccaccatcgt gcggatttta ggatgagaag aaacgggcag gtgcagctgt tgttgtggct
    52861 gctgagggag ttcatcaaac aggacctgaa ttaatctgcg ggtcatcggc tcagcacgct
    52921 gagtcgtcgt cctgtttgcc agggttaaaa tcaattcccg gcataagggc gatattttca
    52981 atgtgcaaca tgttgtcggc attgtcactg cgctgggttc gataaacaga aaacagagtt
    53041 cagcgttagc tgtcacttga ttgctatgtt ccaccccgcc cggtatccaa accgcatatt
    53101 gcggcggcac catccacaaa gcattttcca ccgtacaggt aattgcacca tgtaatgcta
    53161 gtatcagctg tcctttgcgg tgctgatgta caggactggt taactcatcc gttccggcgt
    53221 gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg ttcatagcca ttgagattca
    53281 acctgtgcat cattttgtcc gaatttagcg ataatttgtc attttagctt gattcaacat
    53341 aacaataaaa acggtaaggt acagcctcgt ttgtaacaat gagaagcata tgacctgttc
    53401 aacttcatta agcggcaaaa acaggattgt ccttatcgct ggcattctga tgattgccac
    53461 tacattacgc gtcaccttca ccggcgcagc accgttactc gatacgattc gttccgctta
    53521 ctcgctgacg acagcgcaaa ccggcttatt aaccaccctg ccattattgg cctttgcgct
    53581 aatctcacct ttggctgccc cggtggcgcg acgttttggt atggaacgta gcctgtttgc
    53641 cgcgttactt ttgatctgtg caggtatcgc aattcgttct ctcccttcgc cttacttatt
    53701 atttggcggt acagtggtca ttggcggtgg gattgcatta ggcaatgtct tactgccagg
    53761 attaattaaa cgcgatttcc ctcattccgt cgccagactt accggcgcat attccctgac
    53821 aatgggagct gcagcggcac tgggatcggc tatggtcgtg ccgctggctt tgaacggttt
    53881 tggctggcaa ggcgcgttgt tcatgctgat gtgttttcct ctgctggctc tttttttatg
    53941 gctgccacag tggcgaagcc aacaacatgc aaatttgagt acctcgcgcg ccttacatac
    54001 tcggggtatc tggcgttcgc cgcttgcctg gcaggtcaca ttgtttcttg ggatcaactc
    54061 actggtctat tacgtgatta ttggctggct tccggcgatc ctcatcagtc gcggctatag
    54121 cgaagcacag gcgggttcac tgcatggttt gctgcaacta gccacagcag ctcccggttt
    54181 gctgatccct cttttcttac atcatgtgaa agatcagcgt ggtattgcag cgttcgttgc
    54241 cttgatgtgc gcagtgggcg ctgttgggct ctgctttatg ccagcgcacg cgatcacctg
    54301 gactctgctt ttcggttttg gttccggcgc aacaatgata ctggggttga cgttcattgg
    54361 tcttcgggca agttctgcgc atcaggcggc ggcactctcg gggatggcac aatccgtcgg
    54421 gtatttgctg gcagcctgtg ggccgccgct gatgggtaaa atacacgatg ctaacggtga
    54481 ctggtccgta ccactgatgg gtgttgccat actttcacta ctgatggcga ttttcggact
    54541 ttgcgccggg agagacaaag aaattcgcta atatccggtg ctatagtgac gtgacaaatc
    54601 atgcgtgaaa gggagaacaa acacgatgaa tattcagtgc aaacgcgttt atgatccggc
    54661 tgaacagagc gatggttatc gcgtactggt cgaccgcctc tggccgcgcg gtatcaaaaa
    54721 aaccgattta gcccttgatg agtgggataa agaaatcacg ccgtcaacgg aactgcgcaa
    54781 agcctttcac ggcgaagtcg tcgattttgc aacctttcgc gagcaatatc ttgcagaact
    54841 ggcgcaacac gagcaagaag gaaagcggct ggcggacatc gccaaaaaac agccgctgac
    54901 cctgctctac gcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg ccgactggct
    54961 acgtagcttg tgattttagt acagcatccg gcggttattt ttcaccagcc ggatggtccc
    55021 gtcgccacaa tgcccattca tcaatcgttt caccgcccgg taatttgcaa ttgttgctga
    55081 ccccttgcgc tgtctgcact ggaatgagcg tcccgccctt ctgctggcaa tagaccgacg
    55141 ccggatttgc cataccaatc tgcggcggtt taggtgcttc tggctgagaa gaggttgaac
    55201 aaccagccag gaccagcaag caaggcaaag caaaactgat aattttcatt tattgatctc
    55261 acacatttat ctacgcttag agtatcgcgg tatcgttttg ttttgcagca ctatttttat
    55321 tacattcact caaaacatat tacgtcttgt ttcatctttg ttgatgatgt tttatcatgc
    55381 ctgcaaagat taaataatca gcatttaccc gccgtatcct ggagttgttt cgtgtcagat
    55441 cagattatcg cccgcgtctc gcaatccctt gccaaagaac agtcactaga aagtctggtc
    55501 cgacagcttc tggagatgct ggaaatggtc actgatatgg aatcaaccta cctgaccaaa
    55561 gtggatgtcg aagcgcgcct gcagcatatt atgtttgccc gtaacagcca gaaaatgcac
    55621 atcccggaga attttaccgt ctcgtgggat tactcgttat gcaaacgcgc tattgatgaa
    55681 aactgctttt tcagcgatga agtccctgac cgttggggcg actgtattgc ggcacgcaat
    55741 cttggaatca ccacatttct gagtacacca attcacttac cggatggatc attctatggc
    55801 acgctttgcg ccgccagcag tgagaagcgc cagtggagtg aacgcgcgga acaggtttta
    55861 cagttattcg ccggactgat tgcacaatat attcaaaaag aggcgttggt tgaacagctg
    55921 cgcgaagcca atgccgcgct gattgcgcaa tcgtataccg actcgttaac cgggctaccg
    55981 aatcggcggg cgatttttga aaatctgacg acgctgtttt ctctcgcccg gcatcttaac
    56041 cataagataa tgatcgcgtt tatcgatctg gataacttca aattaatcaa tgatcgtttt
    56101 ggtcataata gtggcgatct gtttctcatt caggttggcg agcgccttaa tacgctccag
    56161 caaaatggcg aagttattgg tcgtctcggc ggtgatgagt ttttggttgt ttcactaaat
    56221 aacgagaatg cggatatttc gtcgctgcga gaacgtattc aacagcaaat acgtggagaa
    56281 tatcacttag gtgatgttga tttgtattat cctggtgcca gtcttggcat tgtagaagtc
    56341 gatcctgaaa cgaccgatgc agacagtgcc ctgcatgctg ccgatatcgc gatgtatcag
    56401 gagaaaaaac acaaacagaa aacacctttt gtcgcgcatt cagcgccaca ttcctgaggc
    56461 gtattcacat ccttttgatt ggtgataaca tgcgaatcgg tattattttt ccggttgtaa
    56521 tctttattac agcggtcgta tttttagcat ggttttttat tggcggctat gctgccccgg
    56581 gagcataaag atgaaaaaaa caacgattat tatgatgggt gtggcgatta ttgtcgtact
    56641 cggcactgag ctgggatggt ggtaacgtca cctctaaaaa atagcaaagg ctgcctgtgt
    56701 gcagcctttg tgcaatttaa gcgttaactt ttaatcttcc tgtagataaa tagcacgaca
    56761 atcgcaccaa taacggcaac cacgaagctg ccaaaattga agccatcgac tttaccaaag
    56821 ccaaacagcg tgctgatcca tccgccaact acggcaccga ctatccccag caggatagtc
    56881 ataaagaatc caccaccatc tttacctggc atgatccact tcgccagaat accggcaata
    56941 agcccaaaaa taatccatga cagaat
//
LOCUS       DAZGTR010000017        50493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGTR010000017 DAZGTR010000000
VERSION     DAZGTR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.017"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            100..2739
                     /gene="ydbH"
                     /locus_tag="ACHLCY_001187"
     CDS             100..2739
                     /gene="ydbH"
                     /locus_tag="ACHLCY_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP0368494.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQMEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNSEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            2736..2921
                     /gene="ynbE"
                     /locus_tag="ACHLCY_001188"
     CDS             2736..2921
                     /gene="ynbE"
                     /locus_tag="ACHLCY_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP0368495.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            2926..3255
                     /gene="ydbL"
                     /locus_tag="ACHLCY_001189"
     CDS             2926..3255
                     /gene="ydbL"
                     /locus_tag="ACHLCY_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP0368496.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(3427..4332)
                     /gene="feaR"
                     /locus_tag="ACHLCY_001190"
     CDS             complement(3427..4332)
                     /gene="feaR"
                     /locus_tag="ACHLCY_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFP0368497.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADNEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            4568..6054
                     /gene="feaB"
                     /locus_tag="ACHLCY_001191"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /pseudo
     CDS             4568..6054
                     /gene="feaB"
                     /locus_tag="ACHLCY_001191"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
     gene            complement(6112..8385)
                     /gene="tynA"
                     /locus_tag="ACHLCY_001192"
     CDS             complement(6112..8385)
                     /gene="tynA"
                     /locus_tag="ACHLCY_001192"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFP0368498.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(8633..10678)
                     /gene="paaZ"
                     /locus_tag="ACHLCY_001193"
     CDS             complement(8633..10678)
                     /gene="paaZ"
                     /locus_tag="ACHLCY_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFP0368499.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRTDSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            10963..11892
                     /gene="paaA"
                     /locus_tag="ACHLCY_001194"
     CDS             10963..11892
                     /gene="paaA"
                     /locus_tag="ACHLCY_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFP0368500.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            11904..12191
                     /gene="paaB"
                     /locus_tag="ACHLCY_001195"
     CDS             11904..12191
                     /gene="paaB"
                     /locus_tag="ACHLCY_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFP0368501.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            12200..12946
                     /gene="paaC"
                     /locus_tag="ACHLCY_001196"
     CDS             12200..12946
                     /gene="paaC"
                     /locus_tag="ACHLCY_001196"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFP0368502.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            12961..13458
                     /gene="paaD"
                     /locus_tag="ACHLCY_001197"
     CDS             12961..13458
                     /gene="paaD"
                     /locus_tag="ACHLCY_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFP0368503.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            13466..14536
                     /gene="paaE"
                     /locus_tag="ACHLCY_001198"
     CDS             13466..14536
                     /gene="paaE"
                     /locus_tag="ACHLCY_001198"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFP0368504.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGERLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            14533..15300
                     /gene="paaF"
                     /locus_tag="ACHLCY_001199"
     CDS             14533..15300
                     /gene="paaF"
                     /locus_tag="ACHLCY_001199"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFP0368505.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            15300..16088
                     /gene="paaG"
                     /locus_tag="ACHLCY_001200"
     CDS             15300..16088
                     /gene="paaG"
                     /locus_tag="ACHLCY_001200"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFP0368506.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            16090..17517
                     /gene="paaH"
                     /locus_tag="ACHLCY_001201"
     CDS             16090..17517
                     /gene="paaH"
                     /locus_tag="ACHLCY_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFP0368507.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            17507..17929
                     /gene="paaI"
                     /locus_tag="ACHLCY_001202"
     CDS             17507..17929
                     /gene="paaI"
                     /locus_tag="ACHLCY_001202"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFP0368508.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            17929..19134
                     /gene="paaJ"
                     /locus_tag="ACHLCY_001203"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             17929..19134
                     /gene="paaJ"
                     /locus_tag="ACHLCY_001203"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFP0368509.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            19161..20474
                     /gene="paaK"
                     /locus_tag="ACHLCY_001204"
     CDS             19161..20474
                     /gene="paaK"
                     /locus_tag="ACHLCY_001204"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFP0368510.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            20575..21525
                     /gene="paaX"
                     /locus_tag="ACHLCY_001205"
     CDS             20575..21525
                     /gene="paaX"
                     /locus_tag="ACHLCY_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFP0368511.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            21507..22097
                     /gene="paaY"
                     /locus_tag="ACHLCY_001206"
     CDS             21507..22097
                     /gene="paaY"
                     /locus_tag="ACHLCY_001206"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP0368512.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVESNRKRLVFDENLRPKQ"
     gene            22328..23188
                     /gene="pdxI"
                     /locus_tag="ACHLCY_001207"
     CDS             22328..23188
                     /gene="pdxI"
                     /locus_tag="ACHLCY_001207"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP0368513.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            23252..25558
                     /gene="ydbD"
                     /locus_tag="ACHLCY_001208"
     CDS             23252..25558
                     /gene="ydbD"
                     /locus_tag="ACHLCY_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP0368514.1"
                     /translation="MFKSRICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            25729..26334
                     /gene="ynbA"
                     /locus_tag="ACHLCY_001209"
     CDS             25729..26334
                     /gene="ynbA"
                     /locus_tag="ACHLCY_001209"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0368515.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            26334..27229
                     /gene="ynbB"
                     /locus_tag="ACHLCY_001210"
                     /pseudo
     CDS             26334..27229
                     /gene="ynbB"
                     /locus_tag="ACHLCY_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
     gene            27245..29002
                     /gene="ynbC"
                     /locus_tag="ACHLCY_001211"
     CDS             27245..29002
                     /gene="ynbC"
                     /locus_tag="ACHLCY_001211"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP0368516.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     LVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            28992..30308
                     /gene="ynbD"
                     /locus_tag="ACHLCY_001212"
     CDS             28992..30308
                     /gene="ynbD"
                     /locus_tag="ACHLCY_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP0368517.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(30359..30964)
                     /gene="azoR"
                     /locus_tag="ACHLCY_001213"
     CDS             complement(30359..30964)
                     /gene="azoR"
                     /locus_tag="ACHLCY_001213"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP0368518.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            31165..35067
                     /gene="hrpA"
                     /locus_tag="ACHLCY_001214"
     CDS             31165..35067
                     /gene="hrpA"
                     /locus_tag="ACHLCY_001214"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP0368519.1"
                     /translation="MTEQQKMTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            35339..36139
                     /gene="ydcF"
                     /locus_tag="ACHLCY_001215"
     CDS             35339..36139
                     /gene="ydcF"
                     /locus_tag="ACHLCY_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP0368520.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            36336..37638
                     /gene="aldA"
                     /locus_tag="ACHLCY_001216"
                     /pseudo
     CDS             36336..37638
                     /gene="aldA"
                     /locus_tag="ACHLCY_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(37680..38681)
                     /gene="gap"
                     /locus_tag="ACHLCY_001217"
     CDS             complement(37680..38681)
                     /gene="gap"
                     /locus_tag="ACHLCY_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0368521.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            38870..39400
                     /gene="cybB"
                     /locus_tag="ACHLCY_001218"
     CDS             38870..39400
                     /gene="cybB"
                     /locus_tag="ACHLCY_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP0368522.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            39645..39818
                     /locus_tag="ACHLCY_001219"
     CDS             39645..39818
                     /locus_tag="ACHLCY_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368523.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            39844..39912
                     /gene="yncP"
                     /locus_tag="ACHLCY_001220"
     CDS             39844..39912
                     /gene="yncP"
                     /locus_tag="ACHLCY_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP0368524.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(39890..40039)
                     /gene="hokB"
                     /locus_tag="ACHLCY_001221"
     CDS             complement(39890..40039)
                     /gene="hokB"
                     /locus_tag="ACHLCY_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP0368525.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            40438..42078
                     /gene="trg"
                     /locus_tag="ACHLCY_001222"
     CDS             40438..42078
                     /gene="trg"
                     /locus_tag="ACHLCY_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP0368526.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASATHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(42116..43039)
                     /gene="ydcI"
                     /locus_tag="ACHLCY_001223"
     CDS             complement(42116..43039)
                     /gene="ydcI"
                     /locus_tag="ACHLCY_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0368527.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELELLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            43256..44599
                     /gene="ydcJ"
                     /locus_tag="ACHLCY_001224"
     CDS             43256..44599
                     /gene="ydcJ"
                     /locus_tag="ACHLCY_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0368528.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            44824..46479
                     /gene="opgD"
                     /locus_tag="ACHLCY_001225"
                     /gene_synonym="mdoD"
     CDS             44824..46479
                     /gene="opgD"
                     /locus_tag="ACHLCY_001225"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP0368529.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            46619..46843
                     /gene="ydcH"
                     /locus_tag="ACHLCY_001226"
     CDS             46619..46843
                     /gene="ydcH"
                     /locus_tag="ACHLCY_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP0368530.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            46906..47442
                     /gene="rimL"
                     /locus_tag="ACHLCY_001227"
     CDS             46906..47442
                     /gene="rimL"
                     /locus_tag="ACHLCY_001227"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP0368531.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(47437..48416)
                     /gene="ydcK"
                     /locus_tag="ACHLCY_001228"
                     /pseudo
     CDS             complement(47437..48416)
                     /gene="ydcK"
                     /locus_tag="ACHLCY_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
     gene            48540..49532
                     /gene="tehA"
                     /locus_tag="ACHLCY_001229"
     CDS             48540..49532
                     /gene="tehA"
                     /locus_tag="ACHLCY_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP0368532.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            49529..50122
                     /gene="tehB"
                     /locus_tag="ACHLCY_001230"
     CDS             49529..50122
                     /gene="tehB"
                     /locus_tag="ACHLCY_001230"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP0368533.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            50425..>50493
                     /locus_tag="ACHLCY_001231"
     CDS             50425..>50493
                     /locus_tag="ACHLCY_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP0368534.1"
                     /translation="MRTTSFAKVAALCGLLALSGCAS"
BASE COUNT        12445 a        11937 c        13332 g        12779 t
ORIGIN      
        1 atgcttagtg taggcgcaac cttcaactga acggttaaac atgccacaat acccgtattg
       61 aatgcttaat ttttcgctaa atcaggatat taactaccca tgctgggtaa atataaagcc
      121 gttctcgcgc tgttattact gattattctt gtgccgttga cgctgttgat gacgctcggg
      181 ctgtgggttc ccactctggc gggcatctgg ctaccgctcg ggacacgtat tgcattagat
      241 gaaagcccac gcattacgcg taaaggttta atcattcccg atctccgtta tctggtggga
      301 gattgtcagc ttgcgcatat caccaacgcc agcctttcac atcccagccg ctggttattg
      361 aacgtcggca cggtagaact tgattctgct tgtctggcga aattgccgca gatggagcaa
      421 tcgccagctg ctccaaaaac cctcgcgcag tggcagtcca tgctgcctaa cacctggatc
      481 aatatcgata aactgatttt ttctccctgg caggaatggc agggaaaact ctctctcgca
      541 ttaacctctg atatccagca actgcgttat cagggcgaaa aagttaaatt tcaaggccag
      601 ctgaaagggc aacaacttac agtcagcgaa ctggatgtcg tcgcgtttga aaatcagccg
      661 ccggttaaac tggtggggga atttactatg ccgctcgtgc cggatggact tcctgtaagt
      721 gggcatgcta ctgcgacgtt aaacttgccg caggaaccgt cactggtgga tgcagagctg
      781 gactggcagg aaaatagcgg gcaattgatt gtgctggcac gggataacgg cgatccgttg
      841 ctcgatttgc cgtggcaaat tactcgtcaa caattgaccg taagcgatgg tcgctggagt
      901 tggccgtatg caggttttcc tttgagtggc cgactgggtg tcaaagtcga caactggcag
      961 gcagggcttg agaacgctct gatcagcgga cgactgagtg tgctgaccca ggggcaagcg
     1021 ggtaaaggca acgcggtgct aaattttggc ccaggaaaat taagcatgga taacagccag
     1081 ctgcctctgc aactgaccgg tgaagcgaaa caggcggacc tcattttata tgcccgctta
     1141 cccgcgcagc taagtggaag tctgactgac ccaacgctgg cctttgagcc aggcgcgtta
     1201 ttacgttcga agggaagagt catcgattcg ctggacatcg atgaaatccg ctggccttta
     1261 gcgggtgtaa aagtcaccca acgtggtgtt gacggacgtt tgcaggccat tttgcaggcg
     1321 catgaaaatg aactgggcga tttcgtgctg catatggatg ggctggcgaa tgattttctc
     1381 cctgacgcgg gccgctggca gtggcgctac tggggaaaag gcagttttac accgatgaat
     1441 gccacctggg atgtcgcagg aaaaggtgaa tggcatgaca gcacgattac gctgaccgac
     1501 ctctccaccg gtttcgacca gttacaatac ggtacgatga cggtagaaaa gccgcgatta
     1561 attctcgaca agcccgtcgt ctgggtacgt gacgcacagc atccctcctt cagcggcgcg
     1621 ctgtcactgg acgccgggca aacgctgttc actggcggca gtgtgttacc gccatcaacc
     1681 ttaaaattta gcgtcgatgg gcgcgatccg acgtatttcc tctttaaagg cgatttacat
     1741 gctggtgaga ttggcccggt tcgggtaaat ggtcgctggg acggtattcg tctgcgcggt
     1801 aacgcctggt ggccaaaaca atcactgact gtattccagc cgctggtgcc acccgactgg
     1861 aagatgaact tacgcgatgg tgaactgtat gctcaggttg cattttctgc tgcgcctgaa
     1921 caaggattcc gcgcgggagg acacggcgtg ttgaaaggcg gtagtgcctg gatgccagat
     1981 aatcaggtta acggtgtcga ttttgtcctg cctttccgtt ttgccgatgg agcctggcat
     2041 ctggggactc gcggccccgt tacgttgcga attgccgaag tgattaatct ggtgacagcg
     2101 aaaaatatta cggctgattt gcaagggcgt tatccgtgga ctgaagaaga acccttgctg
     2161 ttgactgatg ttagcgtcga tgtgttaggc ggtaacgtac tgatgaaaca attacgtatg
     2221 ccgcaacatg acccggcgct gttgcggctg aataatctct catccagcga actggttagc
     2281 gccgtcaatc cgaaacaatt cgccatgtcc ggggcattta gtggtgcatt gccgttatgg
     2341 ctaaacagcg aaaaatggat agtgaaagat ggttggctgg caaatagcgg gccaatgacc
     2401 ttgcggctgg ataaagacac cgcagatgcg gtggtgaaag acaatatgac tgcgggttca
     2461 gcaattaact ggttgcgcta tatggaaatt agccggtcat cgacaaaaat taacttagat
     2521 aatctcggtt tattaaccat gcaggccaac attacaggta ccagtcgcgt tgatggtaaa
     2581 agcggtacgg taaaccttaa ttaccatcat gaagagaata tttttacgct gtggcgcagt
     2641 ttacgctttg gcgataatct ccaggcatgg ttggagcaga acgcacgtct gccgggaaat
     2701 gactgtccgc aaggaaaaga gtgtgaggaa aaacaatgaa aattttactg gctgcgttga
     2761 cgtcatcttt tatgctggct gcctgtacgc cgcgtattga agtcgctgcg cccaaggaac
     2821 cgatcactat caatatgaac gttaaaattg agcatgagat catcatcaag gcagacaaag
     2881 atgtcgaaga gctgcttgaa actcgtagcg atcttttctg aggcgatgat gaagaaaaca
     2941 ttactttttt gtgcgtttct tgttgggctg gtaagcagca atgtaatggc attgactctg
     3001 gatgaagcca gaactcaggg gcgggtaggt gaaacatttt atggttatct ggttgcgctg
     3061 aaaacggatg ctgaaacaga gaaattagta accgacatta atgccgaacg taaagcgagt
     3121 taccaacaac tggcaaagca aaataatgtg tctgtagatg atatcgcgaa actcgccggg
     3181 caaaagctcg tagctcgggc caaaccgggg gaatatgtac aagggataaa cggtaaatgg
     3241 gtgcgaaaat tttaatagca aaaccgcaat atcgggataa cgctacagga gaaagcgatt
     3301 gatctatttt cctgaaacaa ggtgaatatt caaaaactcc tgtcaaattg cctttcgccc
     3361 tgaaaaatgc atagtcaaat atctgtttta actaattggc gttgcagtac acacaacgcc
     3421 aattagttag cggaatttac gtcgatactc gcctggcgtc atcccaaagc gttgcttaaa
     3481 taccgttgaa aaatgactct ggtcagaaaa tccccaatga aagccgatgc ctgccagttt
     3541 ttcattatct gcggcatggc gaatcgcatc tgcacaaaaa tcgagacgac ggttacgaat
     3601 atattgcgcg actaccaaac ctttatcggc aaacattcgg tacaaactac gtactgacat
     3661 acctgtctct ccggctatcc actccgggcg taatatctct tcgcgaatat tatcgtctat
     3721 caacgtaacc actttttgaa actgacgttc acgacgaggt tgaacagatt cccgctgatg
     3781 aagtaccggg cgcagcagac acaccatcgc ctgtagcgca gcttcacttt ctgtttcaga
     3841 aagtgccgga ttattcatgc tctcctgtaa caggcgatga ctgagttgca ccatgggtaa
     3901 gtcagcgtcc agtctttctg cgcagacagg tttttgatgg ggaaaatatt gttccagcag
     3961 agtgcgtggc aaaagtaatg aaatctgttt agaagactcc tgccagtaaa gcgaacaggg
     4021 gcgtgaggca tcgagtaacg taatatcgcc agcgccaatc tgcacctgac gctcatcctg
     4081 ctccattatt gcctgaccac taagctgaaa aacggtgtaa aaccaggcat cgtcgctgcc
     4141 ttttacttcc tgccaggtgc gggataaatt caccccgctg gttgtcacgg tactcagctt
     4201 tagtcctttg gcaaaatgcg tatccagtac acccgtgtaa cgctcagtca gcaggtgtcc
     4261 ggtaaaattt ccgcatacct gattgatttg ggaaagccat tgctgaaact cattatccac
     4321 tgcggggttc atggcacgtt ttcgctctgt gaaatgtatt tttattgttg catttgtgtt
     4381 gcagtaaacg aagctaatga gcctgactat aggaaataag tcttctcagg catagagaca
     4441 taagcggtta ttgtcacgat ttgcggagct tgtcacagct gacaaagcga atgtcacagc
     4501 gaaaaaagtg acttttcttg tcgctgcgta cactgaaatc acactgggta aataataagg
     4561 aaaagtgatg acagagccgc atgtagcagt attaagccag gtccaacagt ttctcgatcg
     4621 tcaacacggt ctttatattg atggtcgtcc aggccccgca caaagtgaaa aacgattggc
     4681 gatctttgat ccggccaccg ggcaagaaat tgcgtctact gctgatgcca acgaagcgga
     4741 tgtagataac gcaggcgggc ctttgtctcg cgtcgctggg ccgggcgatt acccgcagag
     4801 cgtgaacgta ttctgctacg ttttgctgat ctggtggagc agcacagtga ggagctggcg
     4861 caactggaaa ccctggagca aggcaagtca attgccattt ctcgtgcttt tgaagtgggc
     4921 tgtacgctga actggatgcg ttataccgcc gggttaacga ccaaaatcgc gggtaaaacg
     4981 ctggacttgt cgattccctt accccagggg gcgcgttatc aggcctggac gcgtaaagag
     5041 ccggttggcg tagtggcggg aattgtgcca tggaactttc cgttgatgat tggtatgtgg
     5101 aaggtgatgc cagcactggc agcaggctgt tcaatcgtga ttaagccttc ggaaaccacg
     5161 ccactgacga tgttgcgcgt ggcggaactg gccagcgagg ctggtatccc tgatggcgtt
     5221 tttaatgtcg tcaccgggtc aggtgctgta tgcggcgcgg ccctgacgtc acatcctcat
     5281 gttgcgaaaa tcagttttac cggttcaacc gcgacgggaa aaggtattgc cagaactgct
     5341 gctgatcact taacgcgtgt aacgctggaa ctgggcggta aaaacccggc aattgtatta
     5401 aaagatgctg atccgcaatg ggttattgaa ggcttgatga ccggaagctt cctgaatcaa
     5461 gggcaagtat gcgccgccag ttcgcgaatt tatattgaag cgccgttgtt tgacacgctg
     5521 gttagtggat ttgagcaggc ggtaaaatcg ttgcaagtgg gaccggggat gtcacctgtt
     5581 gcacagatta accctttggt ttctcgtgcg cactgcgaca aagtgtgttc attcctcgac
     5641 gatgcgcagg cacagcaagc agagctgatt cgcgggtcga atggaccagc cggagagggg
     5701 tattatgttg cgccaacgct ggtggtaaat cccgatgcta aattgcgctt aactcgtgaa
     5761 gaggtgtttg gtccggtggt aaacctggtg cgagtagcgg atggagaaga ggcgttacaa
     5821 ctggcaaacg acacggaata tggcttaact gccagtgtct ggacgcaaaa tctctcccag
     5881 gctctggaat atagcgatcg cttacaggca gggacggtgt gggtaaacag ccatacctta
     5941 attgacgcta acttaccgtt tggtgggatg aagcagtcag gaacgggccg tgattttggt
     6001 cccgactggc tggacggttg gtgtgaaact aagtcggtgt gtgtacggta ttaatctggt
     6061 tcgctcataa gtaaaaaacg gcacctggtg ccgttttttt gtctgaaaca atcacttatc
     6121 tttcttcagc gcccctagcg ttggcgtttc gtcaaagaag ttccatggtt taagcagagt
     6181 atgtacccat tcggtcggca taatcggcca ctcttcggcg cgggccacat gtgtggtgcc
     6241 ggtggtcatc cagacaacgg cgtcggtgtt gtccagcgac tcgttatctt tactgtattg
     6301 tccaagaccg gtgtcatgag tagaacggtt cggatatttg ccttccggga aacgctcgcc
     6361 aggatgataa cgcgttaccc aaagctgctt gtccataaag cttaaacgat gatagatcca
     6421 ctcgtccggc gcgaactggg caccttttgc taccgggtga gtaccacctg cataaggaat
     6481 aatttgatag gaaactggat tgcccatgcg gttctctttg ttcgggttac tcaacagacg
     6541 aatcgtgccc ggatcaaact tctgcgcggc atcctgttca ttgccgatgt tgtactgatt
     6601 aacttgcatg gtactggtgc gtgggccacc ggcagtattc ggttttacca ctgggtccat
     6661 cgccaccagg ctgttattct cgccatctac atccagatcg aggcggaaat tataaatatg
     6721 ttggtgtgta gtacccacga tattgtgatc gataagcgtg ccgtagcgcg tgtcatcttt
     6781 cgccgtctca tcgtgcatgg ttttcgcttt aacacctttc accgcttcga tgcccgtagc
     6841 accggcatca atgccaatag tgccgttttc atggaagatc cagtcaaaaa tgtagtcata
     6901 gttacccact gtactgatcc agcgcaccac taactcccgg cgttcggtac tgacgttggg
     6961 ctggcccatt tcctgatgct tatactcagg cccggcataa cgttcaaata ccgcgatagc
     7021 gcgagggatc tccatcggca cgccagtgta gtcggcgatg gtttcattaa ggagcactgc
     7081 gttagacggg gcatctttac cacgagcaat tggtgaggtt agcgtgccca taccgtagtc
     7141 accagagtcc agatacgctt taaagtacca gccaatatca ggatcaccgt aaggcacaat
     7201 catgccgccg agagaacctt cgtacatgac tttgcgtttg gtgccattgt cgttataagt
     7261 cacggtggag atcatcggcc cgacgcgaga gttcatgctg aggtgaaaat cccagttccg
     7321 ccagtgaatc atatcgccag taatggtgta atttttacct tcaggctcaa tgatttgcat
     7381 aggcttaact gccggagcaa cgcggtcacg gccatcaaat gggcgtgcgg tcattggcac
     7441 cggaactacc ggaccttctt caatcttaac gatttttttc tgttctaaat caacgaccgc
     7501 caccaggttt tcgatgggat gtgcccagta gttgccatca ccgacatcaa gatagctgat
     7561 gactttgagc aaccgggcat cttgtttcag gccatcttta ccatcgaaat aacctacggt
     7621 cagcggcgtg gtaatcactt ttttcgcatc agtaataccg cgtttcttca cggcagcggc
     7681 aaattcttca ctgttgttaa taatgttctg cacactggcg aaatcatcca gcaacaccat
     7741 accgtgggcg tctttaatgg gttgccagga gagcagtttg ttgttttgca gatccactac
     7801 cgcttcgatg atatgtttgc cgtcgagcat aatgacgtcg gctttgcgtg gctggtcaac
     7861 cggtttgttt tccagcgcaa acgcccagac agcttcttta tctggcggta gcagggagat
     7921 ctcagtaaaa cgggtattgg gtttgaagtc cgcggaagct ttaacaattt caacggcctg
     7981 tttaatttcg tccgcagtta gcgcattaag tgggtgaggg cgcttttcta cctgaaaggt
     8041 ttgatccaga ccggactgga aaacatcgtt aataaaggtg tcagaaaccc aggccttatt
     8101 gtctttcatc actaccggca cttgcagtgc cagaggttga ccattaacaa ttgctgtttg
     8161 cgcaccaggc ttcactttca cgtacgcgcc atctttaatc agggtaaaga tctgggcgta
     8221 gtcgtcccac tgcacatcgg caccaaattc tttaagcgtt tcatccattg gcaccatatg
     8281 cgcttcacca ccgtgggcaa ataccggcgc ttgccaggcg aaacttaagg cgactgccaa
     8341 cgccagggtt gttttacggg cagaatacag agaggggctt cccattatta acctcgtcag
     8401 atgttgtgtt cttgttagca accgcgctct gtgggcggtt tagtcaggtt cacattatca
     8461 gtactgatgc aaaggggatt gcctgcacct gccaggttgt ttggcaggtg tgccagcttt
     8521 tcatacagtg ggtgccctga aaatagatgt acacatcatg cataatgtga caacgtcaca
     8581 aaacttagtg aaataaaagg gcaactattg gccgttgccc ttcattcacc gattaatcga
     8641 caaaatcacc gtgctgcctg gccaccagcg tcagaattga atacagcgcc accggggttt
     8701 gatgctgatt gaatacctct accgcccatt ccaccacacc tgttggtttt tcttctgcgc
     8761 tacgctgttt tttcagcgtc ttgcgcttac aggtgagacg cacctggatg gtatcgcctg
     8821 gctttacggg ttcgataaaa cgcaagcttt ccagcccgta gttagcaatg accggaccaa
     8881 caccggcatc gacaaacaga cccgcagccg cagaaagcac aaaataccca tgcaccaccc
     8941 gctcaccgaa aatagattcg gcagcagcaa tcttatccat atgtgcatag aaatgatcgc
     9001 cgctgaggca agcaaagtta acaatatcgg cctctgtcat tgtgcggcgg ggagtcaaca
     9061 ggctgtcgcc tggttgtagc tcctcaaaat atttgcggaa cggatgaata cgatcttctt
     9121 cgactttcgc accgcgcacc cactgtttac tgatagctgc aagcatcgtc ggactaccct
     9181 gaacagcggt tcgctgcatg taatgtttca ccgctcgtaa accacctaat tcttcaccgc
     9241 ctcctgcgcg accaggccca ccatgtacca gttgtggcag tggggagcca tgcccggtgg
     9301 attcttttgc cgactcttca ttgaggatct gaattcgccc atgcgtacgt gccgcgtcgg
     9361 caataaactg acgcgcaatt tgcggatcag ccgtcaccag cgttcccgca aggctaccgc
     9421 cgcctgcaca agccagttgc agagcatgtt gctggttttg tgctggcatt agcgttgcga
     9481 cagggccaaa ggcttctgtt gcatgtaccg ccggtgtttc atccggctgc ggacagtaca
     9541 ataaggttgg cgggaagaag gcacccgcag cagataaatc cgcctgacca ccgaggcgaa
     9601 tctcgcatcc tgcagccagc aatatgttca ctttttcctg cacatcggca cgctgctcag
     9661 catttaccag tgcgcccatt ttcacgcctt cctgagcagg atcaccgacc acgactttct
     9721 gtaatcgcgc aaccagagca tcactgacag cattaaccaa tgcctgcggc acaataatcc
     9781 gccggattgc cgtacatttt tgcccggctt ttgtggtcat ctcacgcaca acttcacgaa
     9841 taaacagcgc aaactccggt tgatccgggg tgacatcttc gcccagtacg cagcagttca
     9901 gggaatcagc ttccatagtg aaggggatag atttggcgac gatatttggc tgaactcgca
     9961 gcatctgtcc ggtcgccgct gaccccgtga aagtcaccac atcctggctg tccagatgat
    10021 ccaacaagtc gccagcacta ccgcagatca gactaattgc gccttcggga acaagaccac
    10081 tatcgacaat tgatttcacc atcgcctgag tcagttgggc cgtcgcggta gctggtttga
    10141 tgatggctgg cattccgccc agccacgttg gtgccagctt ttccagcatt ccccagcagg
    10201 ggaagttaaa ggcgttaata tgcactgcca cgcctgactt cgaggtcagt acatggcgcg
    10261 cggcaaatcc accttctttc gataagggga tcaattcatc ttccggccac agcgtatcgt
    10321 caggcagctc ccggctaccg aggctggcgt aagtaaataa cgtcccaatg ccaccttcaa
    10381 tatcaaccca actgtctgtc cgcgttgcgc ctgtttgcgc agaaagagca tagaaacgct
    10441 ctttttcact cagcagatgt ttagcgaccg ctttaagcat cgccgcacgt tcgataaagg
    10501 tcatcgcgcg gagggcgggg gcaccttttt caatggcaaa ctggcgggca gccgccatat
    10561 caagaccttc actggtcact tcccataacg cctcgccgct aatagcgtgg tgaatcaaac
    10621 ggctacggcc ccggccagac tgccaggtac cggataagaa actggctaac tgctgcatcg
    10681 ctactctcca gatgtttcac atttctgttg ctaatagtta aatcgcgaat cataaaaagc
    10741 aagggatctt ttaacgaaat gttaactgcg tgatctgtat atcaactact aaaaacatta
    10801 atgcactgat aaataatgag ttatcaaaat agagtgcgaa atctgtcaca gttcaagtat
    10861 acaaaatttg tgactttagt taaccattgt gtaactttca taaaacaacg tgattcgtgt
    10921 ttttaattaa ttcacgaaaa ctggaatcgt aaaggtgatg acgtgaccca agaagaacgc
    10981 tttgagcaac ggatagccca ggaaacggct atcgagccgc aggactggat gcccgatgct
    11041 taccgaaaga cattgatccg ccagattggg cagcatgcgc actccgagat tgttggtatg
    11101 ttgcctgaag gtaactggat cactcgcgca ccaaccttgc ggcgtaaagc cattctgtta
    11161 gccaaagtgc aggatgaagc cggtcatggt ctgtatctct atagcgccgc cgaaacactg
    11221 ggctgtgcca gagaagacat ctaccaaaaa atgctcgacg gacggatgaa atattcctcc
    11281 atctttaatt atccgacatt gagttgggct gatatcggcg ttatcggctg gctggtggat
    11341 ggcgcagcga tcgtcaatca ggtggcgtta tgccgaactt cttatggccc gtatgccaga
    11401 gcaatggtga aaatctgcaa agaagagagt tttcaccagc gtcaggggtt tgaagcctgc
    11461 atggcactgg cgcagggcag cgaagctcaa aagcagatgt tgcaagacgc cattaaccgt
    11521 ttctggtggc cagccttaat gatgttcggg ccaaacgatg ataactcgcc aaacagcgcc
    11581 agaagtctcg cctggaaaat caaacgtttc accaacgacg aactccgcca gcgtttcgtg
    11641 gacaacaccg ttccacaggt tgaaatgctc ggtatgaccg ttcctgaccc ggatctgcat
    11701 tttgacactg aaagcggtca ctaccgcttt ggtgagatcg actggcagga gtttaacgaa
    11761 gtaattaacg gtcgcggaat ttgtaatcag gagaggctcg acgccaaacg taaagcctgg
    11821 gaagaaggta cctgggtacg ggaagcagcg ctggcccatg cacaaaaaca acttgcccgt
    11881 aaggtcgcat aaggagattc aaaatgagta atgtttactg gccgttatac gaagttttcg
    11941 tgcgtggcaa acagggctta tcacaccgcc atgttggcag tttacatgct gccgatgagc
    12001 ggatggcact ggaaaatgcc cgtgatgctt acacccgtcg tagcgaagga tgttcaattt
    12061 gggtggtgaa ggcgagtgaa attgttgcct cgcaaccgga agaacgcggt gaattttttg
    12121 atccggctga aagcaaggtc tatcgccatc caacgtttta caccatccct gatggcattg
    12181 agcacatgtg aggtcggaaa tgaatcagtt aacggcttac accttgcgcc tgggcgataa
    12241 ctgtctggtg ctctcccagc ggttggggga atggtgcggt cacgcaccgg aactggaaat
    12301 cgatctcgca ctggcaaaca ttggcctcga tttattaggt caggcacgta acttcttatc
    12361 gtatgccgct gaattagcgg gagaaggtga tgaagatacc ctggccttca cccgagacga
    12421 gcgccagttc agcaacttat tgttggttga acaaccaaac ggcaattttg ccgacaccat
    12481 tgcacgccag tatttcatcg atgcatggca tgtggcgctc tttacccgtc tgatggaaag
    12541 ccgtgatccg caactggcgg cgatttctgc caaagcaatt aaagaagcgc gttatcacct
    12601 gcgttttagt cgtggctggc tggagcgact gggcaatggt actgacgtat cagggcaaaa
    12661 gatgcagcag gccatcgaca agttgtggcg ttttactgcc gaactgttcg atgccgacga
    12721 gattgatatt gcactgagtg aagagggtat tgcggttgat ccacgcactt tacgcgcagc
    12781 gtgggaagcc gaagtttttg ccggaatcaa cgaagccaca ttgaacgtac cgcaagagca
    12841 ggcgtatcgc actggcggta aaaaaggact gcataccgaa caccttggac ccatgctggc
    12901 agaaatgcag tatctccagc gtgtcttgcc cggtcagcaa tggtaacaga ggagatgggt
    12961 atgcaacgtc tggccaccat tgcaccgcca caggttcatg agatatgggc gctactcagc
    13021 cagatcccgg acccggagat cccggtgctg accattaccg atttaggcat ggtgcgtaat
    13081 gtgacacaga tgggagaagg atgggtgatc ggctttacgc cgacttattc cggttgtccg
    13141 gcaacggaac atttgattgg cgcgatacgt gaggcaatga caacccacgg gtttaccccc
    13201 gttcaggttg tgctgcaact cgacccggca tggaccaccg actggatgac ccccgatgcc
    13261 cgtgagcgtc tgcgacagta tggcattagc ccgcccgccg gacacagttg ccatgcccat
    13321 ttgccgccag aagtacgttg cccgcgctgc gccagcgtcc ataccacact tatcagtgaa
    13381 tttggttcca cggcctgcaa agcattgtac cgctgcgata gttgccgcga acctttcgat
    13441 tatttcaaat gtatttgagg atgccatgac aacgtttcat tccttaacag tggcaaaagt
    13501 cgagccggaa acccgtgatg cggtgaccat tacctttgcg gtgccccagc ctttgcagga
    13561 ggcgtatcgc tttcgccccg gtcaacattt gaccttaaaa gccagtcttg atggtgaaga
    13621 attacgccgt tgttactcca tttgccgcag ctacctgcct ggcgaaatta gtgtggcggt
    13681 gaaagccatt gaaggcggac gtttctcccg ctatgcccgc gaacacatcc gccagggtat
    13741 gacgctggag gtcatggtgc cgcaggggca tttcggctat cagccacagg ccgaacgcca
    13801 gggccgctat ctggctattg cagcaggatc aggtattacg ccaatgctgg cgattatcgc
    13861 caccacttta caaaccgagc ctgaaagtca gttcaccctg atctacggta accgtaccag
    13921 ccagagcatg atgtttcgcc aggcactggc agacctgaaa gacaaatatc ctcagcgttt
    13981 acagttgttg tgcattttca gtcaggaaac cctcgacagc gatctgcttc acgggcgtat
    14041 tgacggtgaa agattacagt cacttggggc ctcgctcatt aattttcgtc tttatgatga
    14101 ggcatttatt tgtggtccgg cggcgatgat ggatgagacg gaagccgcct taaaagcact
    14161 gggaatgcca gataaaacca ttcatctgga gcggtttaat acgcctggca cgcgcgtcaa
    14221 acgtagcgtt aacgtgcaaa gtgacggaca aaaagtgact gtacgtcagg atgggcggga
    14281 tcgggaaatc gtgcttaatg ccgacgatga aagcattctc gatgcggcat tgcgccaggg
    14341 ggctgatctg ccctatgcct gcaaaggcgg cgtctgtgcg acctgcaaat gcaaagtgct
    14401 gcgtggcaaa gtggcgatgg aaaccaatta cagtctggaa ccggatgaac tggccgcagg
    14461 ttatgtgttg agttgccagg cactgccgct gaccagcgat gtggtggttg actttgacgc
    14521 gaaggggatg gcatgagcga actgatcgtc agccgtcagc aacgagtatt gttgctgacc
    14581 cttaaccgtc ccgccgcacg taatgcgcta aataatgccc tgctgatgca actggtaaat
    14641 gaactggaag ctgcggctac cgataccagc atttcggtct gtgtgattac cggtaatgca
    14701 cgcttttttg ccgctggggc cgatctcaac gaaatggcag aaaaagatct cgcggccacc
    14761 ttaaacgata cacgtccgca gctatgggcg cgattgcagg cctttaataa accacttatc
    14821 gcagccgtca atggttacgc gcttggggcg ggttgcgaac tggcattgtt gtgcgatgtg
    14881 gtggttgccg gagagaacgc gcgttttggg ttgccggaaa tcactctcgg catcatgcct
    14941 ggcgcaggcg gaacgcaacg tttaatccgt agtgtcggta aatcgttagc cagcaaaatg
    15001 gtgctgagcg gagaaagtat caccgcccag caagcacagc aggccgggct ggttagcgac
    15061 gtcttcccca gcgatttaac cctcgaatac gccttacagc tggcatcgaa aatggcacgt
    15121 cactcgccgt tggccttaca agcggcaaag caagcgctgc gccagtcgca ggaagtggct
    15181 ttgcaagccg gacttgccca ggagcgacag ttattcacct tgctggcggc aacagaagat
    15241 cgtcatgaag gcatctccgc tttcttacaa aaacgcacgc ccgactttaa aggacgctaa
    15301 tgatggaatt catcctcagt catgtagaaa agggcgtgat gacactaacg ctcaaccgcc
    15361 cggaacgcct gaacagtttt aatgatgaga tgcacgcaca actggcagag tgcctgaaac
    15421 aggtcgagcg cgacgacact atccgttgcc tgttacttac aggtgccggg cgcggatttt
    15481 gtgctggtca ggatcttaac gatcgtaacg ttgatcccac aggccccgca ccggatttag
    15541 ggatgtcagt tgaacgtttc tataacccac tggtacgtcg cctggcaaaa ctgccaaaac
    15601 cggtgatctg tgcagtcaat ggcgtggcgg caggggcagg cgcaacactg gcactggggt
    15661 gcgacatcgt tattgctgcc cgttcagcaa aattcgtcat ggcgtttagt aagttaggct
    15721 taatacccga ttgcggtgga acctggttac tgccacgcgt tgctgggcga gcgcgcgcca
    15781 tggggctggc actgctgggg aatcaactga gtgctgaaca ggcgcacgaa tgggggatga
    15841 tctggcaggt tgttgatgat gaaacgctgg cagataccgc gcaacagctg gcacggcatc
    15901 tggcgacaca accgacattt ggtcttggac ttatcaagca agcgataaat agcgctgaaa
    15961 ccaatacgct cgatacgcaa ctggatctgg aacgcgacta tcagcgactt gccggacgga
    16021 gcgcagatta tcgtgaaggt gtcagtgcgt tcctggctaa acgctcaccg cagttcacgg
    16081 ggaaatagca tgatgataaa tgtgcaaact gtggcagtga ttgggagcgg caccatgggg
    16141 gctggcattg ctgaagttgc cgccagtcat ggacatcagg ttttactgta tgacatttct
    16201 gctgaagcgc tgacccgcgc aatcgacggg atacacgcgc ggctaaattc acgcgtgacg
    16261 cggggaaaac tgactgctga aacctgtgaa cgcacattga aacgcctgat cccggtgacc
    16321 gatattcacg cgctggcagc tgcggacctg gtcattgaag cggcgtctga acgtctggaa
    16381 gtcaaaaaag cgctctttgc acagctggcg gaagtttgcc cgccacaaac gctattgacc
    16441 actaacactt cgtcaatctc tataaccgcg attgctgcgg aggtaaaaaa tcctgaacgt
    16501 gttgcggggc tgcatttttt taacccggca ccggtgatga agttggtgga ggtggtcagt
    16561 gggctggcaa cggcagcgga agttgttgag cagttgtgtg aactaacgtt gagttggggt
    16621 aagcagcctg tgcgctgtca ttcgacccct ggatttatcg ttaaccgtgt ggcgcgtcct
    16681 tattattccg aggcctggcg ggcactggaa gagcaggttg ctgcaccaga agtgattgac
    16741 gctgcacttc gcgatggcgc tggtttcccg atggggccgc tggaattaac cgatctgatt
    16801 ggtcaggacg tcaattttgc tgtcacctgt tcggtgttta acgctttctg gcaggagcgt
    16861 cgttttttac cttcgctggt gcaacaggaa ctggtgattg gtggacggtt gggcaagaaa
    16921 agtgggctgg gcgtgtacga ctggcgcgcg gaacgtgagg cagttgttgg cctggaagcg
    16981 gtaagcgaca gttttagccc aatgaaagta gaaaagaaaa gtgacggcgt cacggaaatt
    17041 gacgatgttt tattgattga gacacaaggc gagacggcac aggcgctggc aatacgactg
    17101 gcacgcccgg tggtagtggt cgacaaaatg gcgggcaagg tggtgaccat tgctgcggca
    17161 gcggtgaacc cggactcagc gacccgcaag gccatttatt acctgcaaca gcagggcaaa
    17221 acagtgctgc aaattgcaga ttacccagga atgctgattt ggcgaacggt agcaatgatc
    17281 atcaatgaag cccttgatgc gctgcaaaaa ggcgtggcct ctgaacagga tatcgatacc
    17341 gccatgcgtc ttggggtgaa ttatccatat ggcccgcttg cctggggagc gcaacttggc
    17401 tggcagcgaa tattaaggct cctggaaaat ctacaacatc actatggcga agaacgctat
    17461 cgcccatgtt cattgctgcg ccaacgagcg cttctggaga gcggttatga gtcataaggc
    17521 ctggcaaaat gcccatgcaa tgtatgagaa cgatgcctgc gccaaagcgc ttggcatcga
    17581 cattatctca atggatgaag gctttgctgt agtgaccatg accgtcactg cacaaatgct
    17641 taacggtcat caaagttgcc acggcgggca gctattttca ctggctgata ctgcctttgc
    17701 ctacgcctgc aatagccagg ggctggcagc cgtcgcttct gcctgcacga ttgatttttt
    17761 gcgtccaggc tttgccggag acaccttaac tgctactgcg caggtacgtc atcagggcaa
    17821 gcaaaccggt gtttacgaca tcgaaattgt taaccaacaa caaaaaacgg ttgcgctgtt
    17881 tcgcggtaaa tctcaccgca tcggcggcac cattacagga gaagcctgat gcgtgaagcc
    17941 tttatctgtg acggaattcg tacgccaatt ggccgctatg gtggggcatt atcaggtgtt
    18001 cgggctgacg atctggctgc tatccctttg cgggagctgc tggtgcgaaa cccgcgtctc
    18061 gatgcggagt gtatcgatga tgtgatcctc ggctgtgcta atcaggcggg agaagacaac
    18121 cgtaacgtag cccggatggc gacgttactg gcggggctgc cgcagagtgt ttccggcaca
    18181 accattaacc gcttgtgtgg ttccgggctg gacgcactgg ggttcgccgc acgggcgatt
    18241 aaagcgggcg atggcgattt gctgatcgct ggtggcgtgg agtcaatgtc tcgggcaccg
    18301 tttgttatgg gcaaggcaac cagtgcattt tctcgtcagg ctgagatgtt tgataccact
    18361 attggctggc gatttgtgaa cccgctcatg gctcaacaat ttggaactga cagcatgccg
    18421 gaaacggcag agaatgtagc tgaactgtta aaaatatcgc gagaagatca agatagtttt
    18481 gcgctacgca gtcagcaacg tacggcaaaa gcgcaatcct caggcattct ggctgaggag
    18541 attgttccgg ttgtgttgaa aaacaagaaa ggcgttgtaa cagaaataca acatgatgag
    18601 catctgcgcc cggaaacgac gctggaacag ttacgtgggt taaaagcacc atttcgtgcc
    18661 aatggggtga ttaccgcagg caatgcctcc ggggtgaatg acggagccgc tgcgttgatt
    18721 attgccagtg aacagatggc agcagcgcaa ggactgacac cgcgggcgcg aatcgtagcc
    18781 atggcaaccg ccggggtgga accgcgcctg atggggcttg gtccggtgcc tgcaactcgc
    18841 cgggtgctgg aacgcgcggg gctgagtatt cacgatatgg acgtgattga actgaacgaa
    18901 gcgttcgcgg cccaggcgtt gggtgtacta cgcgaattgg ggctgcctga tgatgcccca
    18961 catgttaacc ccaacggagg cgctatcgcc ttaggccatc cgttgggaat gagtggtgcc
    19021 cgcctggcac tggctgccag ccatgagctg catcggcgta acggtcgtta cgcattgtgc
    19081 accatgtgca tcggtgtcgg tcagggcatc gccatgattc tggagcgtgt ttgagcatat
    19141 caacctgcga gtaccctaca atgataacca atacaaagct tgacccgatt gaaaccgcgt
    19201 ctgttgatga gttacaggcg ttgcaaacac aacgtctgaa atggacgctc aaacacgcgt
    19261 atgaaaatgt cccgatgtat cggcgcaaat ttgacgcagc aggcgtacat cctgatgatt
    19321 tcagggaact ttcagatctg cgtaaatttc cctgtaccac caaacaggat ctgcgcgaca
    19381 actatccctt cgacaccttt gccgtgccga tggaacaagt ggtgcgcatt catgcttctt
    19441 cgggaaccac aggtaaaccg acagttgtcg gctatacgca aaacgatatt gataactggg
    19501 ccaatattgt agcgcgttcc ttgcgtgcgg caggtggcac gcccaaagac aaaattcatg
    19561 ttgcctatgg ttacggcctg tttactggtg ggctgggcgc gcactatggt gccgaacgtc
    19621 tgggcgctac ggtgatcccg atgtctggtg ggcagacgga aaaacaagcg caactgatcc
    19681 gcgattttca accagatatg atcatggtta cgccatctta ttgccttaac ctgattgaag
    19741 agctggagcg gcagttgggc ggtgatgcca gcggttgctc gctgcgggtt ggagtgtttg
    19801 gtgctgagcc gtggacacag gccatgcgta aagagattga acggcgtctg gggatcaccg
    19861 cactggatat ttatggcctg tcagaagtga tggggccggg ggtggcgatg gagtgtctgg
    19921 aaactaccga cggcccaacc atttgggaag atcatttcta tcctgaaatt gttaatcctc
    19981 atgacggcac accgcttgcc gatggtgaac atggcgaact gttattcacc acgctgacca
    20041 aagaagcatt gccggtcatt cgttaccgca cgcgtgattt aacccgactg ttaccaggaa
    20101 cggcgcggac tatgcgccgc atggatcgca tcagcggacg cagcgatgac atgttgatca
    20161 ttcgcggtgt aaatgtcttc ccgtcacaac tggaagaaga gattgtcaaa ttcgaacatt
    20221 tatcgcccca ttaccaactg gaggtgaacc gccgggggca tcttgattca ctttctgtga
    20281 aagtggagtt gaaagaaagc agcctgacat tgacgcatga gcagcgttgc caggtgtgcc
    20341 accagctgcg ccatcggatt aagtcgatgg tggggatctc taccgatgtg atgatcgtta
    20401 actgtggcag tatcccgcgt tcagaaggca aggcgtgtcg ggtgtttgat ctgcgcaata
    20461 ttgttggtgc ctgacgtgtc gtctggccct ggtgaggtaa agcgccaggg ccagaagtcg
    20521 atacgacctg tgctatgatt cataaatcac aacaataaca acagactgaa tcgaatgagt
    20581 aaacttgtta cttttatcca acatgctgta aacgctgttc cggtcagtgg cacatctttg
    20641 atctcctctc tgtatggtga ttcgctttcc catcgtggtg gtgaaatctg gctgggtagt
    20701 ctggctgctt tgctggaagg gctgggattt ggtgagcgtt tcgtgcgcac cgctttgttt
    20761 cgtcttaata aagaaggctg gctggatgtt tcccgcatcg ggcgacgcag tttctatagc
    20821 ctcagtgata aaggcttgcg cctgacgcga cgggcagaaa gtaaaattta tcgcgcagag
    20881 caacctgcat gggatggtaa atggctcctg ttgctctcgg aaggtttaga taaatcaacg
    20941 ctggctgatg tcaaaaagca gttgatctgg caaggttttg gcgcactggc acccagcctg
    21001 atggcatcgc cgtcgcaaaa actggcggat gtacagacac ttttgcatga agcgggggtg
    21061 gcggataacg tgatttgttt tgaagcgcaa ataccactgg cgctttctcg tgcagcactg
    21121 cgtgccagag tggaagagtg ctggcattta actgaacaaa atgccatgta cgaaaccttt
    21181 attcagtcat tccgcccgct ggtgccgctg ttaaaagagg cagcagacga gttaaccccg
    21241 gagcgtgcat ttcatattca gcttttactg atccattttt atcgccgtgt cgtccttaaa
    21301 gacccattgt tgccagagga gttgcttccg gcgcactggg cagggcatac ggcgcgtcag
    21361 ctgtgtatca acatttatca gcgcgtagcg cctgctgctt tagcgttcgt tagtgaaaaa
    21421 ggtgaaacct cggtcggtga actgcctgcg ccgggaagcc tgtattttca acgttttggc
    21481 ggtttgaata ttgaacagga ggcgatatgc caatttacca gatagatggt ctgactccgg
    21541 ttgtgccaga agagagtttt gtccatccga cagcggtatt gatcggcgat gttattctag
    21601 gcaagggcgt ttacgttggg ccaaatgcca gcctgcgtgg cgattttggt cgtatcgtgg
    21661 tgaaagatgg cgcgaacatt caggataatt gcgttatgca cggttttccc gagcaggata
    21721 ctgtcgtgga agaagatgga catattggtc atagcgctat ccttcatggt tgcattatcc
    21781 gccgcaatgc attagtggga atgaacgcgg tagtgatgga cggcgcggtg attggcgaga
    21841 acagcattgt tggcgcatcc gcatttgtga aagccaaagc agaaatgcct gccaattacc
    21901 tgattgtcgg cagcccggcg aaagcgattc gtgaactcag tgagcaggag ctggcatgga
    21961 aaaagcaggg tacgcatgag tatcaggtgc tggtgactcg ttgtaagcag acgttgcatc
    22021 aagttgagcc attgcgggaa gttgaatcga acaggaaacg tctggtattt gatgagaatc
    22081 tgcgaccgaa acagtaacgt ccagatactg aacattctaa tttttagcat gtgataccta
    22141 aaccgcaacg ctgatacagg ttgcggtttt tttattgccg gatgcggcgc tttacgcgtt
    22201 ttgttttgtg tttttcagga caatagagca actcatccag taatcttgtt tacgccctcg
    22261 cattatttat ctcttttcgt ttctatactg atttttctta atccgtttta ttacagggca
    22321 gggtgcgatg agcagcaata catttactct cggtacaaaa tccgttaacc gtcttggtta
    22381 tggcgcgatg caactggcag gtcctggggt ttttggtccc ccaagagatc gccacgtcgc
    22441 tataactgtg ctgcgcgagg cgctggcatt gggcgtcaat cacattgata ccagcgactt
    22501 ttatggtccg cacgtcacca atcagattat ccgcgaagcg ctttatcctt actctgacga
    22561 cctgacaatt gtcactaaaa ttggtgcgcg gcgtggagag gacgcttcct ggttgcccgc
    22621 cttttctccg gcagagttgc aaaaagcggt gcacgataat ctacgtaatc tcggcctgga
    22681 cgtactggat gtggttaacc tgcgcgttat gatgggagat ggtcatggcc cagcggaagg
    22741 atcgattgag gccagcctga ccgtgctggc agagatgcaa caacaaggcc tggtaaaaca
    22801 tattggcctg agcaacgtca caccgacgca ggttgcagag gcgcgcaaga ttgccgaaat
    22861 tgtctgtgtg caaaacgaat acaacatcgc gcaccgtgct gatgatgcaa tgattgatgc
    22921 tttggcccac gatggcattg cctacgtgcc gttcttcccg ctcgggggct ttacaccgct
    22981 gcaatcctct acgctgtcgg atgttgctgc gagcctgggt gcaacaccca tgcaggtggc
    23041 gctggcgtgg ctgttacaac gttcaccgaa tattttgctg atcccaggga cgtcttctgt
    23101 tgcgcattta cgggagaata tggctgctga aaaattgcat ctttctgagg aagtgttgtc
    23161 tacgttggat ggtatttcgc gagaataacg aatatacaaa agggaaagat gcatttccct
    23221 tttgtttctt ttttaatggc atggagtgca tatgtttaag tccaggattt gcggctggat
    23281 aggtttactt cccctgttta tgctgagtct tccagtacag gcggaacttc gatgtgttgc
    23341 aaatacagtt gatattgaac catttttttc tgcggctacc gctgaagata aacaacaagt
    23401 tgaacaagct atcaacagta gcgtgaatct tgtccccttc ggtttatctg catcggactg
    23461 gaaagtgcat cgtggcgatt tagtggtaga aggtaatata gagagtaatc aaaaattgat
    23521 tgttcttggt aatttgacag tcaaaggtaa tatttccact ttctctcttt ctaatccatg
    23581 ggttattctc ggtaacgtga cggcaacgaa tatagttacg gattcaccac tgttaattac
    23641 cggttcgata aatgcaagtg gactggtatt tatcgactca tattacgata atccgtcttc
    23701 gattaagggg agtattaatg cgcgtggaat atttatcaat gacataattg cacctgttgt
    23761 tgcgtcttcg acaaatagtg aattcatggt ccgtgcgagt gacaaaaatg acactgaaaa
    23821 tgtcaaaaaa gcgctgatga taataaatcc tgatgcatat tattgggggc taataaatga
    23881 tgaagatgct ctgaaagaaa tttttaagcg aagcaatatt cgcatggcag ggaatgtctg
    23941 taatcagatg aaaaaagaag cgctgtttcg ccctaagcct tctcctgagt tagtgcagga
    24001 attgcaaatg ctggatgaag gcaacgttgc tgcatttgaa ggacgagata ttgcgacatt
    24061 tgatcttgcc ataatgcgaa ctctcccaag gcttaaagga atttcagcta acctacgcaa
    24121 acaacttatt aatagcaatg atgaacaaac gattgaaagt atggcgagat atatgcctga
    24181 caatgagatt ctggagttga ccgatcagca attgggttac cagcctgttg ttctggggtt
    24241 gctcgaccgt gaaccgctct ctgtcgaaat aatgacgcga atgagtcgtc tacctgatgg
    24301 tgtcggtccg ttgaatcttg cgctacgtga aaatctccct ctggatatcg taatgactct
    24361 ggcgaaaaga gattgggata tgatcattca ggagctttat aaagatgcgt ggttattgcc
    24421 tgaatccatt attgatggct atatccgcag tgatgattcc tctattcgtc aggtcggtgc
    24481 tggaggacaa ctcacctaca atcaggcaat gcagttggcg aacgattcat caaacaatgt
    24541 tgtcacaagc ttagcgttca agctggcaga gatgaaacac catggtcaat tattgcggat
    24601 gacgccacaa gagagtgaca aagttgcagc ctatttatac caaaaattcg agaatgacga
    24661 tgatctaata cgtgtgttat ttttagcatt gccagataac ttacagttta attttgttaa
    24721 aaggatggag aaaaaatccc ctgcctactt ttgctgtcgg gatatgcagg taattcactc
    24781 tgacgctgct ctacaacggt tattgacacg tttcaacgat cctgaggggt ggagtaatct
    24841 ggcgaaaaat cagtatctga gcacgtcgat gaaacaaaaa atttggcaac gtgctttatc
    24901 gcatcggaaa aataatccga aagcagattc agctgcgtat gaaaccagcg ctgatatgat
    24961 tttgtctgag cttatcagcc acggtgaagt tgatgatcag atgctattaa atgccaccgc
    25021 cctgatacgt ttggaagact gggacttttt agagagtgca ttagttagtt gggataattt
    25081 acccgctgtt gtcctcaagg aattacagca aaatacgcca cgcaatgata tttgggcgaa
    25141 gttttttctg aggcaggaaa acagctcccg tgcgcaggtt gatgaagcat tacgcgtcta
    25201 ttatgcactc gaccccgatg cgttagcaca actggatgta ctggcaaaac aaccggatcg
    25261 tatatggtgg agtacactgg cgaaaagcaa tctcacattt ttcaagttcg gcgcacttaa
    25321 caaccgccac acaccgcctg cagtactggc ggcagaaatt gatcccgagt ggtggattgt
    25381 ggcgatgaat aatccccgtt ttccagttga tgtattaaag gcgaggctga aacgtgatcc
    25441 tttgctggcg ttagagcttg ttaaccctga actggattta gtccgccagc tggcgcttaa
    25501 cggtaagacg cgcgcaatac gggaacaagc gatgagaaaa cttgatgagt tgtattgatc
    25561 cacaacgtaa tatttcttaa ccctttgatt aattggggat aattattcct gacagtgagt
    25621 ccccaatacc ttgatatatt ctgaattttt aatgaagcgg cgtgttgcga tatctccgtc
    25681 aggggaattg atgcaccata gcgcaaaccg aattatcaag gattgataat gacgctctac
    25741 cagataaaac cgctctttca gtcgctgtta aggccgacga tgttttggct ttacaagcac
    25801 cacgttacag cgaatcacat cactcttgct gcactggcgc tttctcttct taccggattg
    25861 ctgttgatgt tggtggcaca acccatcctc tttatgctat tgcccatcgt gctttttatc
    25921 cgtatggcgc tcaatgcgct ggatggcatg ttggcgcgtg agtgcaatca gcaaacacgg
    25981 cttggggcaa ttttgaatga gactggtgat gttatttccg atattgcgct ctatttacca
    26041 tttttatttt taccggaaag taacgcatca ctcgtgatac tcatgttatt ttgcaccata
    26101 ttgaccgagt tttgcggttt actcgcgcag acgattaacg gtgttcgcag ttatgccggt
    26161 ccatttggca aaagcgatcg cgcgttaata tttggtctgt ggggtctggc cgttgccatt
    26221 tatccacagt ggatgcagtg gaataatctt ttatggagca ttgcttcaat tctgcttctc
    26281 tggactgcga ttaatcgttg tcggagtgtg cttcttatga gcgctgaaag ataatgctgg
    26341 aaaaatctct ggcaacactt ttcgcgttgt taattttagc aacgctgata aaccattttc
    26401 tgctatggcg gttaccggag agaaaagagg atgaagttac attacgtatt cgtacctggt
    26461 ggggcatcgt catttgtttt tcactggtga tttcaggtcc acgctggatg acgttgacgt
    26521 tttttgcgct gataagtttt ctggcattga aagaatattg tacgcttata tctgtacatt
    26581 ttccgcggtg gttatattgg gttattcctc ttaactattt gtttatcggt tttaattgct
    26641 ttgagctgta tctattattt atacctttgg ctgggtttct aatattagcc accgggcaag
    26701 tttttatggg ggacccctcc ggttttctgc ataccgtgag cgccattttt tggggctgga
    26761 taatgaccgt tttcgccttg agtcatgccg cctggttatt aatgttgcca accacaaata
    26821 tccagggcgg ggcgttactg gtgttatttc ttttggcatt aaccgagtca aacgatattg
    26881 cacagtattt atgggggaaa tcctgcggca gaagaaaagt ggtccccaaa gtcagcccgg
    26941 gaaaaacgct ggaaggtctg gtggggggcg taatcaccac catgattgcg tcactgatta
    27001 ttggaccgtt gctgacaccg ctaaatacat tacaggcatt attagcgggt ttgttaattg
    27061 gtattagtgg tttttgcggc gatgtcgtga tgtcagccat caaacgagat attggtgtta
    27121 aagatagtgg aaactattac caggacatgg cggactcctt gacaggattg actcattaat
    27181 tttcaccgct ccggtatttt tttattttat tcgctactgc tgttactgaa ggaaaataga
    27241 gaaaatggaa aattcacgca tccatgggga acattttttt accagcagtg ataatacagc
    27301 gttgttttat cggcactggc ccgctttaca gcccggggct aaaaaggtca tcgtcttatt
    27361 tcatcgcggt catgaacatt ctggtcgtct acaacatctc gttgatgaac tggcgatgcc
    27421 agatactgtt ttttatgcct gggatgcccg agggcatgga caaacttcgg ggccgcgtgg
    27481 ttatagccca tctcttgcgc gttcagtgca ggatgtcgat gaatttgttc gttttgctgc
    27541 cagcgatagc ctggtcggac tggatgaggt ggtagtgatc gcgcaaagcg tcggcgcagt
    27601 gctggttgcc acatgggttc atgattatgc gcctgcaatt cgcgggctgg tgctggcttc
    27661 tccggccttt aaggttaaat tgtatgtacc gctggcacgt cctgcgctgg cgttatggca
    27721 tcgtctgcgt ggtctgtttt ttattaattc ctatgtgaaa ggacgctatt tgacccacga
    27781 tcggcaacgg gtggcgagtt tcaataatga tccgctgatc acacgggcga ttgccgttaa
    27841 tatcttgctc gatctctaca aaacgtctga acgtattatt agagatgcgg cggcgattac
    27901 gctccccacg caacttctga tatcaggcga tgactatgtg gtgcatcgcc aaccgcagat
    27961 tgatttttat cagagattac gtagccctct gaaagagctg catctgctgc caggctttta
    28021 tcacgacacg ttgggtgaag agaacagggc gcaggcattt gaaaaaatgc aaagctttat
    28081 tagtcgttta tatgctaaca agtcacaaaa atttgattat cagcatgaag accgcactgg
    28141 accatcagcg gatcgctggc ggctcctttc aggtggaccc gtgccattat cgccggttga
    28201 tttggcgtat cgctttatgc gaaaggcgat gaaattgttc ggggcgcact ctgcgggcct
    28261 gcatctcgga atgagcaccg gctttgattc aggcagttcc ctggattatg tctatcaaaa
    28321 tcaaccgcaa ggcagtaacg cattcgggcg cttaattgac aaaatctacc tgaacagtgt
    28381 tggctggcgc ggtattcgcc agcgtaaaac ccatttacaa atactgatta aacaagccgt
    28441 tgccgatctc cacgccaaag gtttagccgt ccgcgtggtt gacattgccg cagggcatgg
    28501 gcgctatgta ctggatgcgc tggcaaacga gcctgccgta agcgatattt tgttacgtga
    28561 ttacagtgag ttaaatgttg cacaggggca agcgatgatt gcccaacggg gaatgtctgg
    28621 gcgggtgcgt tttgaacagg gcgatgcgtt taacccggag gaactcagcg cgttaactcc
    28681 gcggcctacg ctggcgattg tctctggcct gtatgagctt tttcccgaaa atgagcaggt
    28741 aaaaaactca ctcgcaggtc ttgccaatgc catcgaaccg ggcggcattc tcatctacac
    28801 cgggcagccg tggcaccctc aactggagat gattgccggg gtgttaacca gtcataaaga
    28861 tggtaaaccg tgggtaatgc gcgtgcgttc gcaaggggag atggattcac tcgtgcgtga
    28921 tgccggattt gataaatgca cacaacggat tgatgagtgg ggtattttta cggtttcgat
    28981 ggcggtgcgt cgtgataact gaacgtcgga acgtattgct acaaggcgct ggctggttat
    29041 tgttgctggc cccgtttttc ttcttcacct atggatctct taatcagttc accgcggttc
    29101 aggaccttaa cagccatgat atccccagtc aggtattcgg ttgggaaacg gcgatccctt
    29161 ttcttccctg gactattgtt ccttactgga gtctggatct tttatatgga ttttcgctgt
    29221 tcgtttgtag cacgacattc gaacagcgcc gacttgtcca ccggcttatt ctggcaacgg
    29281 taatggcctg ctgcggtttt ttgctctatc cgctgaagtt tagttttatc cgtcctgaag
    29341 tgagtggggt gacgggatgg ctattttcgc aacttgaact gtttgatctg ccttataacc
    29401 agtctccttc gctgcatatt attctctgct ggctactttg gcgtcacttt cgtcagcatc
    29461 tggctgagag gtggcgtaaa gtctgtggcg gatggttttt actcatcgct atttctactc
    29521 tgacgacctg gcagcatcat tttattgatg tcatcacagg gctggcggta ggtatgttga
    29581 ttgactggat ggtgcccgtc gatcgtcgtt ggaattatca gaaacctgat caacgtcgaa
    29641 tcaaaatagc actgccatat gtcgtaggcg cgggctcgtg cattgtgttg atggagctaa
    29701 tggtgatgat tcagttatgg tggtcagtct ggttatgttg gccagtatta tcgctactca
    29761 ttattggccg tgggtacggt gggcttggcg cgataacaac agggaaagat agtcagggga
    29821 aactcccgcc cgccgtttac tggctgacat tgccctggcg catcgggatg tggctgtcta
    29881 tgcgttggtt ttgtcgtcgc ctggagccgg tgagcaaaat tactgctggt gtttatttag
    29941 gggcgtttcc acgacatatt ccggcacaga atgcggttct ggacgtcacc tttgaattcc
    30001 ctcggggacg agcgacaaaa gatcgactct atttctgtgt accgatgctg gatctggtgg
    30061 ttccggaaga gggggagctc cgacaggccg tggcgatgct ggaaacatta cgcgaagagc
    30121 aaggcagcgt tctggtccat tgcgcgttgg gattatcgcg cagtgcgctg gtagtggcgg
    30181 catggttgtt atgttacgga cactgtaaaa ccgttgatga agcgattagt tatattcgag
    30241 ccagacgttc gcatattgtg cttaaggaag atcacaaagc gatgctgaaa ttatgggaaa
    30301 acaggtaagt ggattgagat gtggactgaa ctacagtcca catcaagatc gtgtccggtt
    30361 atgcagcaac aatgctgtcg atggccgctt tcgcgtcaga ctgcgctttc gctgccattt
    30421 ccggaccgta tgcgatccct tcggcgaaga caaatttcac atcggtaatg ccgataaagc
    30481 cgaggaacgt ggacagatac ggcgtcacca ggtccgttgg tccatctttg tggatcccac
    30541 cgcggctggt aataacgatg gcttttttac ccgttaccag accttccgga ccgttctcgg
    30601 tatagcggaa agtaacgcct gcgcgtgcaa ccaggtcaaa atagtttttc aactgagttg
    30661 agatgttgaa gttatacatc ggtgccgcaa taacgataac gtcgtgggct ttcagctcgg
    30721 caatcagctc atcggaaagc gccagagctt cctgctgacg cggagtcagc ggcgcatcgc
    30781 tcggacgcag tgcaccaacc agttcgccat ccagtaccgg aatcggattt gcagccaggt
    30841 cgcgaacggt gatttcatca gcggagtgct tttcgcgcca ttgttcaaca aaataatcgg
    30901 acaactgatt agactgagag taccctgcca ggatgctgga tttaagaact aataccttgc
    30961 tcatggtgtt tccttataga tgtttgaatg ggcgatgccc cgttgcttgc tgacacttta
    31021 ttcacaatcc tgccacagag atagcgcaat aaatcgaagc ctatgttcga atttattgaa
    31081 caacgcatag aaagccgcga tgtggtactc tatatctatc atttaaaaga aaattaatca
    31141 ggcagactac tgcccactaa cgttatgaca gaacaacaaa aaatgacctt tacggccttg
    31201 cagcagcggc tggattcgct gatgctgcgc gacagactgc gtttttctcg gcgtctgcac
    31261 ggtgttaaga aggttaaaaa tcctgatgca caacaggcca ttttccagga gatggcgaaa
    31321 gagattgacc aggcggcagg gaaagtcctg ctgcgtgaag cggcacgacc ggaaattact
    31381 tatcctgaca atttaccggt tagtcagaaa aaacaggaca ttctcgaagc gattcgtgat
    31441 caccaggtgg tgatcgtcgc cggggaaacg ggttctggta aaacgactca gttaccgaaa
    31501 atctgtatgg agctggggcg cgggattaaa gggctgatcg gccataccca gccgcgtcgc
    31561 ctggcggcaa gaacggtggc gaaccgtatt gcggaagagc tgaaaacgga gccgggcggt
    31621 tgcatcggtt acaaagtgcg tttcagcgat cacgttagtg ataacacgat ggtcaagctg
    31681 atgaccgacg gtatcctgct ggcggagatc cagcaagacc gcctgctgat gcagtacgac
    31741 accatcatta ttgacgaagc acacgaacgc agcctgaata tcgattttct gctcggctat
    31801 ttgaaagagt tgctgccgcg gcgtcctgac ctaaaaatca ttatcacttc cgcgactatc
    31861 gacccggaac gcttttcgcg ccactttaat aatgcgccga ttatcgaagt ctccggtcgg
    31921 acctatccgg tggaagtgcg ctatcgaccg attgttgaag aagccgacga caccgagcgc
    31981 gatcagttgc aggcgatttt tgacgccgta gacgaactga gtcaggaaag ccctggcgac
    32041 attctgatct ttatgagtgg tgagcgggaa attcgcgata ccgccgatgc gctgaacaag
    32101 ctaaatctgc gtcataccga aatcttgccg ctttatgcgc ggctttcgaa cagcgaacag
    32161 aaccgcgtgt tccagtcgca cagcggacgg cgcattgtgc tggcgaccaa cgtcgcggaa
    32221 acctcgctga ccgtaccggg gattaaatac gttatcgacc ccggtacagc acgtatcagc
    32281 cgctacagct atcgcaccaa agtgcagcgt ttgccgattg aacccatttc gcaggcgtct
    32341 gccaatcagc gtaaaggccg ctgtgggcgt gtgtccgaag ggatctgtat tcgtctctat
    32401 tccgaagacg atttcctttc gcgcccggag tttaccgatc cggagattct gcgtaccaac
    32461 ctggcctcgg ttattttgca gatgaccgcg ctggggctgg gcgatatcgc tgcgttcccg
    32521 tttgtcgaag caccggataa acgcaatatc caggatggcg tgcgtctgct cgaagaactg
    32581 ggcgcgatca ccacggatga acaggccagc gcctataaac tgacgccgct cggtcgccag
    32641 ctctcgcagt tgcctgtcga tccacgtctg gcgcgtatgg tgctggaagc gcaaaaacat
    32701 ggctgcgtgc gtgaggcgat gattatcacg tccgcgcttt ccattcagga tccgcgcgag
    32761 cgtccgatgg ataaacagca ggcatcggac gaaaaacatc gtcgcttcca cgacaaagag
    32821 tctgacttcc tcgcgtttgt gaatctgtgg aattatcttg gcgagcagca aaaggcgctt
    32881 tcttccaacg ccttccgtcg cctgtgtcgt accgattatc tcaactatct gcgcgtgcgc
    32941 gaatggcagg atatctacac ccagttgcgc caggtggtga aagaacttgg cattccggtt
    33001 aacagcgaac cggcggagta tcgcgaaatt cacattgcgt tgctgacggg tttactttcc
    33061 catatcggca tgaaagatgc cgataaacaa gaatataccg gcgcacgtaa cgcgcgtttc
    33121 tccatctttc ccggttctgg tttattcaaa aaaccgccga aatgggtaat ggtggcggaa
    33181 ctggtagaaa ccagccgcct gtgggggcgc attgctgcgc gtatcgaccc ggaatgggtg
    33241 gagcctgtcg ctcagcattt gattaaacgc acctacagcg aacctcactg ggaacgggcg
    33301 cagggcgcgg tgatggcaac ggaaaaagtc actgtttatg gtttgccgat tgttgccgcg
    33361 cgcaaggtca actacagcca gatcgatccg gcgttatgcc gtgaactctt tattcgccat
    33421 gcgctggtgg aaggtgactg gcagacgcgt cacgcattct tccgtgaaaa cctgaaactg
    33481 cgggccgaag tggaagagct ggaacacaaa tcacgtcgcc gcgatattct ggttgatgac
    33541 gaaacgttgt ttgagttcta cgaccagcgc atcagccacg atgtaatctc cgctcgtcac
    33601 ttcgacagct ggtggaaaaa agtcagccgc gaaacccctg atttgctcaa ctttgaaaaa
    33661 agcatgttga tcaaagaggg cgcagaaaaa atcagcaagc tggattaccc gaacttctgg
    33721 catcagggca atctcaagct gcgtttgagc tatcagtttg agcccggcgc ggatgctgac
    33781 ggtgtgaccg tacatattcc gctgccgtta cttaaccagg ttgaggaaag cgggtttgaa
    33841 tggcagatcc ccggtctgcg ccgcgaactg gtgattgctc tgattaaatc gttgccgaaa
    33901 ccggtacgcc gtaattttgt acccgcgcca aactatgccg aagcgttttt aggccgcgtc
    33961 aaaccgctgg agttaccgtt gctcgacagc cttgagcgcg agttacggcg gatgaccggc
    34021 gttaccgttg accgcgaaga ctggcactgg gatcaggtgc ctgatcacct gaaaattacc
    34081 ttccgcgtgg tggatgacaa aaacaagaag ctaaaagaag ggcgctcgct acaagatctg
    34141 aaagatgcgc tgaaaggcaa agtgcaggaa acgctatctg cggtggcgga tgacggtatc
    34201 gagcagagcg gcttacatat ctggagtttt ggtcagctgc cggaaagcta cgaacagaag
    34261 cgtggcaact acaaagtgaa ggcgtggccg gcgctggtgg atgagcgcga cagtgtggcg
    34321 atcaaactgt ttgataaccc gctggagcaa aagcaggcaa tgtggaacgg tcttcgccgt
    34381 ctactgctgc tgaatattcc atcgccaatc aaatatttac atgaaaagtt accgaacaaa
    34441 gccaagctgg gactgtactt taacccgtat ggcaaagtgc tggagctgat cgacgactgt
    34501 atctcctgcg gtgtggatca attgatcgac gccaatggtg gcccggtctg gacggaagaa
    34561 ggctttgctg cgctgcatga aaaagtgcgc gccgaactga atgacacggt ggtggatatt
    34621 gcgaagcagg tcgagcaaat cctcactgcg gtgttcaata tcaacaaacg tctgaaaggg
    34681 cgggtggata tgaccatggc actggggctt tctgacatta aagcgcagat gggcgggttg
    34741 gtatatcgcg gttttgtcac tggtaacggc ttcaaacggc tgggcgacac gctgcgatat
    34801 ttgcaggcga ttgaaaaacg gctggaaaaa ctggcggttg atccacatcg cgaccgtgct
    34861 cagatgctga aagtcgaaaa cgtccagcag gcgtggcagc aatggatcaa caaactgccg
    34921 cccgcacgtc gtgaggatga agacgtgaaa gagatccgtt ggatgataga agagttgcgc
    34981 gttagttact tcgctcaaca acttggtacg ccttatccga tttcagataa gcgtattttg
    35041 caggcgatgg agcagattag cggttaaccc tgctatttgc ctgataaaga aaaaacccgg
    35101 taagcattta gcgccgggtt ttttattaat tctaaaacgg taagggtaaa aattcaggaa
    35161 ttcagaaaaa tacaattctc tgctgcaaga tgaataatgt ttatctacag catttcctta
    35221 aaagatatgt caggcttgcg gagtggcggt taaggacata cgacttcctc ctttcagagt
    35281 gctccgcttc tcactattat ctcacgcagt attcttaagg gaacgataag gaggaaccat
    35341 gaacattacc ccgtttccga cgctttcgcc ggcaactata gatgccataa atgttatcgg
    35401 acagtggctg gcgcaggatg atttctccgg tgaggtgccg tatcaggccg attgcgtgat
    35461 ccttgcaggc aatgcggtta tgccgactat cgacgcggca tgtaagattg ctcgcgatca
    35521 gcaaattcct ttactgatta gtggtggtat cggtcactcg acaacttttt tgtatagcgc
    35581 catcgcacag catccgcact acaacactat ccgcaccact ggcagagcag aagcgaccat
    35641 cctggcggat atcgctcatc agttctggca cattccgcat gaaaaaatct ggattgaaga
    35701 ccagtcaaca aactgcggtg aaaacgcacg ctttagcatc gcgctattga atcaggccgt
    35761 agaacgagtt catacggcta tcgttgttca ggaccccacc atgcagcggc gcacgatggc
    35821 gacgttccgc cgtatgactg gggacaatcc cgatgcacca cgctggttaa gttatcccgg
    35881 attcgttcct cagttaggaa ataacgcaga cagtgtaatc tttattaatc agttacaagg
    35941 attatggccg gttgagcgtt atctctcact actcactggc gagctgccgc gtttacgcga
    36001 tgatagcgat ggctacggtc cccgcgggcg agattttatc gttcacgttg attttccggc
    36061 agaagtcatc catgcatggc aaacgctgaa acatgatgcg gtgctcatcg aggcgatgga
    36121 aagtcgctcg ttacgttaaa aattgcccgt ttgtgaacca cttgtttgca aacgggcatg
    36181 actcctgact tttatttctg ccttttattc cttttacact tgtttttatg aagcccttca
    36241 cagaattgtc ctttcacgat gccgtctctc tgatgattga tgttaattaa caatgcattc
    36301 accgaaaaca aacatataaa tcacaggagt cgcccatgtc agtacccgtt caacatccta
    36361 tgtatatcga tggacagttt gttacctggc gtggagacgc atggattgat gtggtaaacc
    36421 ctgctacaga ggctgtcatt tcccgcatac ccgatggtca ggccgaggat gcccgtaagg
    36481 caatcgatgc agcagaacgt gcacaaccag aatgggaagc gttgcctgct attgaacgcg
    36541 ccagttggtt gcgcaaaatc tccgccggca tccgcgaacg cgccagtgaa atcagtgcgc
    36601 tgattgttga agaagggggc aagatccagc agctggctga agtcgaagtg gcttttactg
    36661 ccgactatat cgattacatg gcggagtggg cacggcgtta cgagggcgag attattcaaa
    36721 gcgatcgtcc aggagaaaat attcttttgt ttaaacgtgc gcttggtgtg actaccggca
    36781 ttctgccgtg gaacttcccg ttcttcctca ttgcccgcaa aatggctccc gctcttttga
    36841 ccggtaatac catcgtcatt aaacccagtg aatttacgcc aaacaatgcg attgcattcg
    36901 ccaaaatcgt cgatgaaata ggccttccgc gcggcgtgtt taaccttgta ctggggcgtg
    36961 gtgaaaccgt tgggcaagaa ctggcgggta acccaaaggt cgcaatggtc agtatgacag
    37021 gcagcgtctc tgcaggtgag aagattatgg cgactgcggc gaaaaacatc accaaagtgt
    37081 gcctggaatt gggtggtaaa gcaccagcta tcgtaatgga cgatgccgat cttgaactgg
    37141 cagtcaaagc catcgttgat tcacgcgtca ttaatagtgg gcaagtgtgt aactgtgcag
    37201 aacgtgttta tgtacagaaa ggcatttatg atcagttcgt caatcggctg ggtgaagcga
    37261 tgcaggcggt tcaatttggt aaccccgctg aacgcaacga cattgctgct ggatgttcgc
    37321 caggaaatgt cgattatgca tgaggaaacc tttggcccgg ttctgccggt ggtcgcattt
    37381 gacacgctgg aagatgctat ctcaatggct aatgacagtg attacggcct gacctcatca
    37441 atctataccc aaaatctgaa cgtcgcgatg aaagccatta aagggctgaa gtttggtgaa
    37501 acttacatca accgtgaaaa cttcgaagct atgcaaggct tccacgccgg atggcgtaaa
    37561 tccggtattg gcggcgcaga tggtaaacat ggcctgcatg aatatctgca gacccaggtg
    37621 gtttatttac agtcgtaatg agtaaaagag gcggaggttt tttcctccgc ctgtgcgcgt
    37681 cagagtttag cgaatttttc gagggtgcga ataagctgcg tgacgaagcc atattcgtta
    37741 tcgtaccagg cgaccgtttt caccagttgt aaatcgccca cggcggtaat ttccgtttgc
    37801 gtggcatcaa acaccgaacc gaaatggctg ccaatgatat cggaagagac tatttcttca
    37861 tcggtataac caaatgactc gttattggtg gttgcttgtt taagtgcgtt attcacctct
    37921 tcggcagtca ctttttttcc gagaatggac accagctcag tgaccgaacc tgttttcacc
    37981 ggcacgcgtt gcgcatgacc tttcagtttg ccgctcagtt ccgggatcac cagaccaatg
    38041 gcttttgccg cccccgtagt gtggggaatg atattttctg ccgctgcgcg tgaagcacgt
    38101 aaatctttac cacgtgggcc atccaccagt gactgggtgc cagtataggc atgaatggtc
    38161 gtcatcgtgc cgacttctat cccgaaactg tcatgcaagg ctttggccat cggcgcaaga
    38221 cagttagtgg tgcatgacgc cacggaaaca atggtgtcgt tgccatccag agtgtcgtca
    38281 ttgacgttat aaacgatagt tttcatttca ccggcagggg cggaaatcaa caccttcttc
    38341 gcaccagcat caagatgcgc ctgcgatttc tcggcggagg tataaaagcc agtacattcg
    38401 acaatgattt ctgcaccttt cgctttccac ggaatatttt tagcctcttt ttcggcgtaa
    38461 accgcgatac ttttcccatc aacgataagt gaatcttccg taaaatcaac gctccagggg
    38521 aatggtccgt agtttgaatc atgtttcagc aggtaggcga gaatttttgg ggaagtgaga
    38581 tcattaatag cgacaacgtc tatgttgctt ttgacttcaa gtaatcgacg caacaccagt
    38641 cgaccgatac gaccaaaacc gttaatacca actttactca tggttttctc ctgtcaggaa
    38701 cattcggatg aaaattgatc ctttccaggc ttagaccagg attgtgggat gggcaatctc
    38761 cattcttaca gtgaaacgta acgtactgaa aacgggtgaa caatatttaa tgaaattttg
    38821 agaaaagccc gttatgttaa cggaaaatta tgttaaagca ggaaatgtta tggaaaataa
    38881 atactcaagg ttacaaatca gcattcactg gctggtcttt ttgctggtta tcgcagcgta
    38941 ttgcgcaatg gagtttcgtg gtttcttccc acgtagcgat cggccactca tcaacatggt
    39001 tcatgtttcc tgtggtatct caattctcgt gctgatggtg gttcgtcttt tgttaagact
    39061 gaaataccca accccgccga ttatacctaa gccaaaaccg atgatgacgg gactggcgca
    39121 tttgggacat ttggtgattt atctcctgtt tattgcgctg ccagtgattg gtttggtgat
    39181 gatgtataac cggggcaacc cgtggtttgc gtttggtttg acgatgcctt acgcttcaga
    39241 ggccaatttc gaacgggttg atagcttaaa gtcgtggcat gagacgctgg cgaatctggg
    39301 atattttgtc atcgggttgc acgctgcggc tgcactggca caccactatt tctggaagga
    39361 caacacactt ctacgcatga tgccgcgtaa acgttcctga aggatattta aagaaaacgc
    39421 ctgtactaaa accgacccgt ggtacaggcg aagaatacgg gtctacatcg gaagcgccta
    39481 ttatatttat ttgtatgaca aataaaacca taatccttgc ccatacgtcc atctggctta
    39541 tttttaatca attcacccga tctttgatct catcaacggt atcaaaataa accagcgtat
    39601 aacgttcaaa tcgaacataa gattcgataa accatggagg ttatatgaaa aaactggcac
    39661 ttattttgtt tatgggaacg cttgtttcct tttatgccga tgccgggcgc aaaccctgtt
    39721 ctggttcgaa aggggggatc tcacactgta cggcaggcgg caaatttgtc tgtaatgatg
    39781 gttctattag tgcctcgaaa aaaacatgca ctaactgaag tgtaaaaggg gtgccatgag
    39841 aaaatgaatc tgctagtcag atgcgcgggg aaaatccccg cgcttgccct tacctggacg
    39901 tgcaggccat gagcgcagca acctccttat caccgtcccg gaaccgcagt tcgtagagcg
    39961 tttgtcgggt caggagtgtg aatgtcagaa tcgtaatgca gataatgagc agacacacca
    40021 ccagagggtt gtgcttcata gcctttctcc ttgccggatg gcgggtaaga ggctaagatc
    40081 tgaattgcta ggttcattcg ttggcctcgg ttgatagaaa tatcggtcgg ggccttcgtc
    40141 tttctgattc ccggttagcc tgaaaacaga aagtctcagg cacccgcagg catcctatga
    40201 ggtttcctta gggacgaaaa taatcacttc acgaaattgc gtgctgtttt ccagaatttt
    40261 tcgtcattcg ggttagccag tttagccatt cgttactctc ttcattccaa tagcattaat
    40321 tttctctgca ataattgttg taaaaatgtg acgcaaagag gtttttggtc ataagtaatt
    40381 accgtcaagt gccgatgact ttctatcagg agtaaacctg gacgagagac aacggtaatg
    40441 aatacaactc cctcacatcg attaggtttt ttgcatcaca tcaggctggt tccgttattt
    40501 gcctgcattc ttggcggtat cttagttcta ttcgcattaa gttctgctct ggctggctat
    40561 ttcctctggc aggccgatcg cgatcagcgt gatgttactg cggagattga gattcggacc
    40621 gggttagcga acagttcaga ttttttgcgt tcagcccgga tcaatatgat tcaggccggg
    40681 gctgcaagtc gtattgcgga aatggaagca atgaagcgaa atattgcgca agccgaatcg
    40741 gagattaaac agtcgcagca aggttatcgt gcttatcaga atcgaccggt gaaaacacct
    40801 gctgatgaag ccctcgacac tgaattaaat caacgctttc aggcttatat cacgggtatg
    40861 caacctatgt tgaaatatgc caaaaatggc atgtttgaag cgattatcaa tcatgaaagt
    40921 gagcagatcc gaccgctgga taatgcttat accgatattt tgaacaaagc cgttaagata
    40981 cgtagcacca gagccaacca actggcggaa ctggcccatc agcgcacccg cctgggtggg
    41041 atgttcatga ttggcgcgtt tgtgcttgcc ctggtcatga cgctgataac atttatggtg
    41101 ctacgtcgga tcgtcattcg tccactgcaa catgccgcac aacggattga aaaaatcgcc
    41161 agtggcgatc tgacgatgaa tgatgaaccg gcgggtcgta atgaaatcgg tcgcttaagt
    41221 cgtcatttac agcaaatgca gcattcactg gggatgacag tagggactgt tcgacagggt
    41281 gcggaagaga tttatcgtgg caccagcgaa atttcagctg gcaatgcgga cctgtcatct
    41341 cgcaccgaag aacaagcggc ggctatcgaa caaactgccg ctagcatgga gcaactcact
    41401 gcgacggtga aacagaatgc ggataacgcg catcatgcca gcaaactggc gcaagaggct
    41461 tctattaaag ccagcgatgg cgggcagacg gtttccggtg tagtaaaaac gatgggcgct
    41521 atctctacaa gttcgaagaa aatttccgag atcaccgccg tcatcaacag tattgctttc
    41581 cagacgaata ttctggcact gaatgctgcc gttgaagccg cgcgagcggg tgagcaaggc
    41641 cgtggatttg ccgttgtcgc cagcgaagta cggacactcg caagccgcag cgcccaagcg
    41701 gcgaaagaga ttgaaggctt gatcagtgaa tcagtcaggt taattgacct ggggtcggat
    41761 gaggtggcaa cggcagggaa aaccatgagc actattgttg atgccgtcgc gagtgccaca
    41821 catatcatgc aggaaatcgc cgccgcctcg gatgaacaaa gtagaggcat aacgcaggtt
    41881 agccaggcga tttctgaaat ggataaggtg acgcaacaga atgcttctct ggtagaagag
    41941 gcctcagcgg cggcggtgtc ccttgaagaa caggcggcac gattaactga ggcggtggat
    42001 gtattccgtc tgcacaaaca ttctgtgtcg gcagaacctc gcggagcggg tgaaccagtt
    42061 agtttcgcta cggtgtgaaa atgttcaagg agggatcgac agatcccttc acctttcaga
    42121 acggcattga ttttcgaata gcgttaatca tcaactggca accagaagag aacgtcgcat
    42181 ctacgcgggt cagtattcca atcggttcgc ctgcaccatg tcccggaaca ggcagggcca
    42241 ccagcgtggc atgacgcagg tcgtctttta cagcgccaga agggacaaac cacacgtaat
    42301 cgtattcaac cgtaagttga cgagatagcg aagcagacag cgtttcgata caacccgaag
    42361 gaattttaca gccctggctc tgcactaatg catctgaatg ctggcgtggc gcagtgcctt
    42421 ctggtgatac aacgaccggc cattccagca cccggcttag cgttacgttc tcctgaagta
    42481 gcgggtgatt agggcggaca accagcttca acgattcaag aaacagcagt tcgtaattaa
    42541 gcccggtcat cagttcagga tctgacatcc gaccaatgcc gatatcgatt tccccggttt
    42601 ttaaacccgc cagaatcata gggttactca ttgtcgcaac ttgcaaggtc gtctcttttt
    42661 gttgctgatg aaactgacct ataaccgaag gtaatatccc cagtgccgca gtaggtagtg
    42721 caccaaccct gacgacatca ttattaagac cttctttacg atgaagcgac tgtccggcag
    42781 tgttgatggc gtcaagaact ctgactgcat gcgttaaaaa ttgttcgccg ggtaaggtaa
    42841 gttgcgcccc ctgacgacca cgctcaaaca agcgagcgcc agtcagcagc tccagttcat
    42901 tcaatgtctt agagagcgca ggttgactca aattaagggt ttcagccgcg cgccccaaag
    42961 ttccttgttg tgcgacagct acgaatgtat gaaggtggcg caaacggatg cgctgactaa
    43021 acagactatt tttttccata agcgatgtta aaaacgaagc ggtgtcgctg acaagtgaag
    43081 ttgtttgatt atgataactt gattgcaaaa tattattaac aattaaagca attgtgttac
    43141 agcaaaatgg ataatattga tgttttcgcg gcgagatcac agtttgtaaa ttcttcccgc
    43201 cagagtgaat gcggttacct acactccaga ttactgacca ctggaggcag acactatggc
    43261 gaacagcatc acggcggatg agattcggga acagttttcg caggcaatgt cagccatgta
    43321 ccagcaagaa gttccgcaat atggcacgct gctggaactg gtagctgatg tgaatctggc
    43381 tgtgctggaa aacaatcctc aactgcacga aaaaatggta aatgcagacg agctggcgcg
    43441 actgaatgtt gaacgtcatg gggcgattcg cgttgggact gcacaagagc ttgctactct
    43501 tcggcggatg tttgccatta tggggatgta cccggtgagc tattacgatc tctcgcaggc
    43561 aggggtgccg gtacattcga cagcatttcg gcccattgat gatgcttctc tggcgcgtaa
    43621 tcccttccgc gtttttacct ccttactccg ccttgagctt atcgagaacg aaattttgcg
    43681 ccagaaagcg gcggagattc tgcgtcagcg cgatatcttc accccacgtt gtcgacaact
    43741 gttagaggaa tatgagcagc agggcggttt taacgaaaca caggcacagg agtttgtgca
    43801 ggaagccctg gaaacgtttc gctggcacca gtcagcaacg gtagatgaag aaacctatcg
    43861 cgccttgcac aacgaacatc ggttgattgc tgatgtggtc tgttttcctg gatgccatat
    43921 caaccacctg acgccacgta cgctggatat tgaccgggtg cagtcgatga tgcctgaatg
    43981 cggaattgaa cccaaaattc tcatcgaggg gccgccgcgc cgcgaggtac cgattttact
    44041 acgccagacc agctttaaag cactggaaga gacggtgttg tttgcggggc agaaacaggg
    44101 cacgcatacc gcgcgctttg gtgaaattga gcagcgtggc gtggcattaa cgccgaaagg
    44161 gcgacaactg tatgatgatc ttctgcgtaa cgctggaacc gggcaggata atctcactca
    44221 ccaaatgcat ttacaggaaa ccttccgcac ttttcctgac agtgagtttt taatgcgtca
    44281 gcaagggctg gcatggttcc ggtaccgtct gacgccttca ggtgaggcgc atcgtcaggc
    44341 gattcatccc ggagacgatc cacagccctt aattgaacgt ggttgggtcg cggcgcaacc
    44401 cattacctat gaagatttct tgcccgttag cgcggcgggg atcttccagt caaatctggg
    44461 taatgaaacg caggcacgca atcacggtaa tgccagtcgc gaggcatttg agcaggcgtt
    44521 gggttgtccg gttttggatg agttccagct ttatcaggaa gcggaagaac gcagtaaacg
    44581 tcgctgtggt ttgctttaaa ttctgaccat ccgcctttgc aaaaatttgc ctgattttac
    44641 aaacgaatca ggctcatccc atcgacataa aaaaattgcc gatttatgca tattctctga
    44701 gtttaacaat tggattatta ataaatattg tctagagtga gcggtcataa ataagcactt
    44761 tcttgccgct gaaaacgacc agcgcgggac cattcacaac accagaagga ctcactttca
    44821 ggtatggatc gtagacgatt tattaaaggt tcaatggcta tggccgccgt gtgcggtacc
    44881 agcggcattg cttctctttt ttctcaggcg gcattcgcgg cagattctga tattgccgac
    44941 gggcaaaccc agcgttttga cttctccatt ctacagtcaa tggcgcacga cttagcgcaa
    45001 acagcgtggc gtggtgcgcc tcgtccgtta cctgacacgc tggcgacaat gacgccgcag
    45061 gcttataaca gtattcaata cgacgccgaa aaatcgctct ggcataacgt tgagaaccgt
    45121 caactggacg ctcagttctt ccatatggga atgggattcc gtcgccgcgt tcgtatgttt
    45181 tctgtagatc cagcaacaca tctggcgcgt gaaattcact ttcgcccgga gttgttcaaa
    45241 tacaacgatg caggtgttga taccaaacaa ttagaagggc aaagcgatct cggctttgcc
    45301 ggttttcgcg tgtttaaagc ccccgaactg gcgcgccgtg atgtagtatc attcctcgga
    45361 gcgagttatt tccgcgccgt tgatgacaca tatcaatacg gtttgtcggc ccgcggcctg
    45421 gcgattgaca cttacaccga cagtaaagaa gagttccccg actttaccgc cttctggttt
    45481 gatacggtaa aaccgggggc aactaccttt accgtttatg cgttgctcga tagcgccagc
    45541 attactggtg cctataagtt cactatccat tgtgagaaaa gtcaggtgat tatggatgtg
    45601 gaaaatcacc tgtatgcgcg caaagacatt aaacagctgg gcattgcgcc gatgaccagt
    45661 atgttcagct gcggtactaa tgaacgtcgg atgtgcgaca ccattcatcc gcaaattcat
    45721 gactctgatc gtctgtccat gtggcggggc aacggcgagt ggatttgccg tccgctgaat
    45781 aatccgcaaa aattgcagtt caatgcttac accgacaaca acccgaaagg gtttggttta
    45841 ttgcaactgg atcgtgattt ctcccattat caggacatta tgggctggta taacaaacgc
    45901 ccaagtctgt gggtggaacc gcgtaacaag tggggtaagg gcaccatcgg cctgatggaa
    45961 atcccaacaa cgggcgaaac gctggataac attgtctgct tctggcagcc agaaaaagct
    46021 gtaaaggcgg gtgatgagtt tgcattccag tatcgtctgt actggagtgc gcaaccgcct
    46081 gttcattgcc cattagcgcg cgttatggcg acgcgtaccg gcatgggcgg tttcccggaa
    46141 ggttgggcac caggtgaaca ctatcccgaa aaatgggcgc gtcgttttgc cgtcgatttc
    46201 gttggtggtg atctgaaagc tgccgcacca aaaggcattg agccggtgat tacgctttcc
    46261 agtggggaag cgaagcaaat cgaaattctc tatattgaac ccattgatgg ttatcgtatt
    46321 cagtttgact ggtatccgac ttcggactcc actgatccgg tcgatatgcg gatgtatctg
    46381 cgttgtcagg gcgacgctat cagtgaaaca tggctgtatc agtatttccc gccagcgccg
    46441 gataaacgtc agtatgttga cgaccgcgtg atgagttaat cgttttttct tcggcacttt
    46501 cttcgggagg tgccgtctgg ttaaacacga tcccgctcgc atttttccct aagttaaatg
    46561 agtaatctga tggtgtgtat ttcagataca ccttgtcagc cactaacagg gagtgcgtat
    46621 gtttccagaa tatcgagatt taatatcccg tctgaaaaac gaaaatcctc gctttatgtc
    46681 cttgttcgat aaacacaata aacttgatca tgaaattgcc agaaaggaag gttccgacgg
    46741 tcgagggtac aatgcggaag tggtccgcat gaaaaaacaa aagctacagt taaaagatga
    46801 gatgctcaaa atcctgcagc aggagagcgt caaagaggtg taaactttcc taagccgcca
    46861 gccaggcggc ttttttaaca actgcatgga ttgactggag ataagatgac tgaaacgata
    46921 aaagtaagcg aatcacttga attacatgct gttgcagaaa atcacgtcac tcctctttat
    46981 cagttaatct gtaaaaataa aacctggtta cagcagtcgc taaactggcc gcagttcgtt
    47041 cagagtgaag aggacacgcg aaaaacggtg cagggtaatg tgatgttgca tcaacgcggc
    47101 tatgccaaaa tgttcatgat tttcaaagaa gatgaactta tcggcgttat ctcgtttaat
    47161 cgtattgaac cactgaataa aaccgctgaa ataggctact ggctggacga atctcatcag
    47221 gggcagggga tcatttctca ggcgctgcag gcattgattc atcattacgc ccagtctggt
    47281 gaacttagac gcttcgtgat caaatgtcgg gtggacaatc cgcaaagcaa ccaggtcgct
    47341 ttgcgcaatg gttttatcct tgaaggttgc ctgaaacagg ctgagttcct gaatgatgcc
    47401 tatgatgatg tgaacttata cgcgcgtatt atcgattcat aataaccctg ccagcggcgt
    47461 tcgcgtaatg cgctcctcgc cgttaatgac ttttggacca cggacatgaa ccgtgtcgcc
    47521 atcaaaggct tcaacgacgg cgtggtcggt taactccaca tggttttcaa ttatcaccgc
    47581 acctgtaata cggctctcac cttgtattac aacgtgctcg tcgagcagaa tcggtccacc
    47641 acgtaccacg gcatttccgc caatcaagac atgatgtttt aacacacaat taccttccac
    47701 aatggcatat tccgccacct gcgaactgta atgaatcgtt ggaatggcat cttcttctat
    47761 gccagctttc acctgcgcgt gaccgtagac tttagcgcaa tcgcatagcc agacgttgtt
    47821 ctcttcatta ccttcaatac tggcaaaatc aaaaacttcg gcacgatgtt caataaaagc
    47881 ataccggatg acggcatcgc cataaatttg tgcctggtgg acaatacgcg aggcgctaac
    47941 ccttgcgcga tcataaattt gtagcaggag ttgatggtca ggcgttaagc cttgtgctgc
    48001 gacgatcata gaatgttgat taattaaggc gtgaccaaat attctacatt gcccgcaaac
    48061 cagtgagtca tgaatggtga cactgtcact tatatatgca ccctggctga tttcactatt
    48121 atcgatccag acattatccg ttgcgtaaac ctctccccac agtacgctgg tgccggtgat
    48181 gcgcgtatta ccggaaatca ccgttccgcc gaacgcaata gcattctggt cgtaaatcca
    48241 gcaatttcct tcctgtgcta acaccgtttc gcgatcgatc cagccgccag cggttcctgc
    48301 tatcacatcg ttaaaatcgc taatggcgat aatctgccgt aataacacat ttttttagtg
    48361 ccatcttcct gataactaaa ggctcgctgt tcttcactta gacgatattt gcgcatagcg
    48421 ttttcccaca ggtgacttac tataaccgta gcaaattctg cggctctggc tatgctcgag
    48481 aaaattccat aaaatgcatt tcaaatatac tttataaatt aaacaaaatg agtaagaaga
    48541 tgcagagcga taaagtgctc aatttgccgg caggctactt tggtattgtg ttggggacga
    48601 tagggatggg atttgcctgg cgctatgcca gccaggtttg gcaggtcagc cactggttag
    48661 gggatgggct ggtgattctg gcgatgatca tctggggatt attaactagc gcatttatta
    48721 cccgactcat acgctttccg catagcgtgc tggcggaagt tcgccatcca gtgctgagca
    48781 gttttgtgag tttgtttccg gcaacgacga tgctggtggc gattggtttt gttccgtggt
    48841 ttcgcccact ggcggtgtgc ctgttcagtt ttggtgtcgt ggttcagttg gcttatgccg
    48901 cctggcaaac tgcgggatta tggcgcggat ctcaccctga agaagctacc acgcctggac
    48961 tgtatctgcc gacagttgcc aacaacttta tcagcgcaat ggcctgtggt gcgttgggct
    49021 acaccgacgc cggtctggtg tttttaggcg caggcgtttt ctcatggcta agccttgaac
    49081 ctgtgatctt gcagcgtctg cgcagttcgg gagaattacc cacggcactg cggacatcac
    49141 tcggcattca gctcgctcct gcgctggtgg cctgtagtgc ctggttgagc gtcaacggcg
    49201 gcgagggtga tacgctggcg aaaatgcttt ttggttatgg actgctgcaa ctgctgttta
    49261 tgctacgtct gatgccatgg tatctctccc agccatttaa tgcttcattc tggagtttct
    49321 cgttcggcgt atctgcactg gcaaccaccg gtttgcatct ggggagtggc agcgaaaatg
    49381 gatttttcca tacgctggcg gtaccgctgt ttatctttac caattttatt attgcaatac
    49441 tgctcatccg tacttttgcg cttctgatgc agggaaaatt gttagtcaga accgagcgcg
    49501 ccgttttaat gaaagcagag gacaaagaat gatcattcgt gacgaaaact attttactga
    49561 taaatatgaa ttaacccgca cacactctga agtactggaa gcggtgaaag tggttaaacc
    49621 gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac agtctttacc tggcagccaa
    49681 tggttatgat gttgacgcat gggataaaaa tgccatgagt atcgccaatg tagagcgcat
    49741 taaatccatt gaaaatctgg ataatttaca cacccgagtc gttgatctga ataacctcac
    49801 atttgataga cagtacgatt ttattctttc gactgtggta ctgatgttcc ttgaggctaa
    49861 aaccatccca gggctgattg ccaatatgca acgttgcact aaacctggtg gttacaacct
    49921 gattgtggcg gcgatggata ccgctgatta tccatgtacc gtcggcttcc cgtttgcctt
    49981 caaagaggga gaattacgtc gatattacga aggctgggag agggtgaaat acaatgaaga
    50041 cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt attaaactgc gtttcgccac
    50101 gatgctggca cgtaaaaaat gacccgctaa gaataaggcg cgtgaaaatt ccccacgctg
    50161 agatgattta ctgttcttct tttcggtaag catatttttt atcgaaggga tgtgaaatta
    50221 atcacagtag tcgaagtttt ttagcagctt aacttactga aatttaacta ctgatgattg
    50281 acttagcccc tttttcggca ttgactatgt cgtctgaaaa ggggctgaaa aatttatttt
    50341 caccaacact ttttttgcca caacacgaag cggcgctttt tgctataact taaaaagtaa
    50401 tataatcatc tcaggaaact attcatgcgt accacatcat ttgcgaaagt tgcagcttta
    50461 tgcggcttgt tggctctgtc tggttgtgcc tct
//
LOCUS       DAZGTR010000018        49541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGTR010000018 DAZGTR010000000
VERSION     DAZGTR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.018"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..326
                     /locus_tag="ACHLCY_001232"
     CDS             <1..326
                     /locus_tag="ACHLCY_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0368535.1"
                     /translation="NRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGH
                     TAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEM
                     IQATA"
     gene            370..477
                     /locus_tag="ACHLCY_001233"
                     /pseudo
     CDS             370..477
                     /locus_tag="ACHLCY_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(461..1099)
                     /gene="ytfB"
                     /locus_tag="ACHLCY_001234"
     CDS             complement(461..1099)
                     /gene="ytfB"
                     /locus_tag="ACHLCY_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP0368536.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1318..1938
                     /gene="fklB"
                     /locus_tag="ACHLCY_001235"
     CDS             1318..1938
                     /gene="fklB"
                     /locus_tag="ACHLCY_001235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0368537.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2247..3650
                     /gene="cycA"
                     /locus_tag="ACHLCY_001236"
                     /gene_synonym="dagA"
     CDS             2247..3650
                     /gene="cycA"
                     /locus_tag="ACHLCY_001236"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP0368538.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(3692..3790)
                     /locus_tag="ACHLCY_001237"
                     /pseudo
     CDS             complement(3692..3790)
                     /locus_tag="ACHLCY_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3917..4345
                     /locus_tag="ACHLCY_001238"
                     /pseudo
     CDS             3917..4345
                     /locus_tag="ACHLCY_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4450..5517
                     /locus_tag="ACHLCY_001239"
     CDS             4450..5517
                     /locus_tag="ACHLCY_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368539.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(5764..6426)
                     /gene="ytfE"
                     /locus_tag="ACHLCY_001240"
     CDS             complement(5764..6426)
                     /gene="ytfE"
                     /locus_tag="ACHLCY_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP0368540.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6534..7499)
                     /gene="ytfF"
                     /locus_tag="ACHLCY_001241"
     CDS             complement(6534..7499)
                     /gene="ytfF"
                     /locus_tag="ACHLCY_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0368541.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(7607..8467)
                     /gene="qorB"
                     /locus_tag="ACHLCY_001242"
     CDS             complement(7607..8467)
                     /gene="qorB"
                     /locus_tag="ACHLCY_001242"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0368542.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLANEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            8556..8936
                     /gene="ytfH"
                     /locus_tag="ACHLCY_001243"
     CDS             8556..8936
                     /gene="ytfH"
                     /locus_tag="ACHLCY_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP0368543.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(9065..11008)
                     /gene="cpdB"
                     /locus_tag="ACHLCY_001244"
     CDS             complement(9065..11008)
                     /gene="cpdB"
                     /locus_tag="ACHLCY_001244"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP0368544.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            11198..11938
                     /gene="cysQ"
                     /locus_tag="ACHLCY_001245"
     CDS             11198..11938
                     /gene="cysQ"
                     /locus_tag="ACHLCY_001245"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP0368545.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(11928..12485)
                     /gene="ytfJ"
                     /locus_tag="ACHLCY_001246"
     CDS             complement(11928..12485)
                     /gene="ytfJ"
                     /locus_tag="ACHLCY_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0368546.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDRALTQEEVQQVMDLLHKLINK"
     gene            12810..13016
                     /gene="ytfK"
                     /locus_tag="ACHLCY_001247"
     CDS             12810..13016
                     /gene="ytfK"
                     /locus_tag="ACHLCY_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP0368547.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(13078..14421)
                     /gene="paeA"
                     /locus_tag="ACHLCY_001248"
     CDS             complement(13078..14421)
                     /gene="paeA"
                     /locus_tag="ACHLCY_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP0368548.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14744..15382)
                     /gene="msrA"
                     /locus_tag="ACHLCY_001249"
     CDS             complement(14744..15382)
                     /gene="msrA"
                     /locus_tag="ACHLCY_001249"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP0368549.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            15588..17321
                     /gene="tamA"
                     /locus_tag="ACHLCY_001250"
     CDS             15588..17321
                     /gene="tamA"
                     /locus_tag="ACHLCY_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP0368550.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTRGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            17318..21097
                     /gene="tamB"
                     /locus_tag="ACHLCY_001251"
     CDS             17318..21097
                     /gene="tamB"
                     /locus_tag="ACHLCY_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP0368551.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELTVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     NRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            21100..21441
                     /gene="ytfP"
                     /locus_tag="ACHLCY_001252"
     CDS             21100..21441
                     /gene="ytfP"
                     /locus_tag="ACHLCY_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP0368552.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            21653..21904
                     /gene="chpS"
                     /locus_tag="ACHLCY_001253"
     CDS             21653..21904
                     /gene="chpS"
                     /locus_tag="ACHLCY_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP0368553.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            21898..22248
                     /gene="chpB"
                     /locus_tag="ACHLCY_001254"
     CDS             21898..22248
                     /gene="chpB"
                     /locus_tag="ACHLCY_001254"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP0368554.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(22328..22858)
                     /gene="ppa"
                     /locus_tag="ACHLCY_001255"
     CDS             complement(22328..22858)
                     /gene="ppa"
                     /locus_tag="ACHLCY_001255"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP0368555.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            23168..24124
                     /gene="ytfQ"
                     /locus_tag="ACHLCY_001256"
     CDS             23168..24124
                     /gene="ytfQ"
                     /locus_tag="ACHLCY_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP0368556.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            24264..25766
                     /gene="ytfR"
                     /locus_tag="ACHLCY_001257"
     CDS             24264..25766
                     /gene="ytfR"
                     /locus_tag="ACHLCY_001257"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP0368557.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGPALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            25780..26802
                     /gene="ytfT"
                     /locus_tag="ACHLCY_001258"
     CDS             25780..26802
                     /gene="ytfT"
                     /locus_tag="ACHLCY_001258"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP0368558.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            26789..27784
                     /gene="yjfF"
                     /locus_tag="ACHLCY_001259"
     CDS             26789..27784
                     /gene="yjfF"
                     /locus_tag="ACHLCY_001259"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP0368559.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(27817..28815)
                     /gene="fbp"
                     /locus_tag="ACHLCY_001260"
     CDS             complement(27817..28815)
                     /gene="fbp"
                     /locus_tag="ACHLCY_001260"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP0368560.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            28991..30364
                     /gene="mpl"
                     /locus_tag="ACHLCY_001261"
     CDS             28991..30364
                     /gene="mpl"
                     /locus_tag="ACHLCY_001261"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP0368561.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(30520..31071)
                     /gene="yjgA"
                     /locus_tag="ACHLCY_001262"
     CDS             complement(30520..31071)
                     /gene="yjgA"
                     /locus_tag="ACHLCY_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP0368562.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            31165..32517
                     /gene="pmbA"
                     /locus_tag="ACHLCY_001263"
     CDS             31165..32517
                     /gene="pmbA"
                     /locus_tag="ACHLCY_001263"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP0368563.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            32700..33086
                     /gene="cybC"
                     /locus_tag="ACHLCY_001264"
     CDS             32700..33086
                     /gene="cybC"
                     /locus_tag="ACHLCY_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP0368564.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(33131..33595)
                     /gene="nrdG"
                     /locus_tag="ACHLCY_001265"
     CDS             complement(33131..33595)
                     /gene="nrdG"
                     /locus_tag="ACHLCY_001265"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP0368565.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(33754..35892)
                     /gene="nrdD"
                     /locus_tag="ACHLCY_001266"
     CDS             complement(33754..35892)
                     /gene="nrdD"
                     /locus_tag="ACHLCY_001266"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP0368566.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(36286..37941)
                     /gene="treC"
                     /locus_tag="ACHLCY_001267"
     CDS             complement(36286..37941)
                     /gene="treC"
                     /locus_tag="ACHLCY_001267"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP0368567.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(37991..39412)
                     /gene="treB"
                     /locus_tag="ACHLCY_001268"
     CDS             complement(37991..39412)
                     /gene="treB"
                     /locus_tag="ACHLCY_001268"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP0368568.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(39531..40478)
                     /gene="treR"
                     /locus_tag="ACHLCY_001269"
     CDS             complement(39531..40478)
                     /gene="treR"
                     /locus_tag="ACHLCY_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP0368569.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            40663..40716
                     /gene="mgtL"
                     /locus_tag="ACHLCY_001270"
     CDS             40663..40716
                     /gene="mgtL"
                     /locus_tag="ACHLCY_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP0368570.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            40857..43553
                     /gene="mgtA"
                     /locus_tag="ACHLCY_001271"
     CDS             40857..43553
                     /gene="mgtA"
                     /locus_tag="ACHLCY_001271"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP0368571.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(43759..44145)
                     /gene="ridA"
                     /locus_tag="ACHLCY_001272"
     CDS             complement(43759..44145)
                     /gene="ridA"
                     /locus_tag="ACHLCY_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP0368572.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(44218..44679)
                     /gene="pyrI"
                     /locus_tag="ACHLCY_001273"
     CDS             complement(44218..44679)
                     /gene="pyrI"
                     /locus_tag="ACHLCY_001273"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP0368573.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(44692..45627)
                     /gene="pyrB"
                     /locus_tag="ACHLCY_001274"
     CDS             complement(44692..45627)
                     /gene="pyrB"
                     /locus_tag="ACHLCY_001274"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP0368574.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(45631..45765)
                     /gene="pyrL"
                     /locus_tag="ACHLCY_001275"
     CDS             complement(45631..45765)
                     /gene="pyrL"
                     /locus_tag="ACHLCY_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP0368575.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(46046..46441)
                     /gene="yjgH"
                     /locus_tag="ACHLCY_001276"
     CDS             complement(46046..46441)
                     /gene="yjgH"
                     /locus_tag="ACHLCY_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0368576.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(46572..47285)
                     /gene="bdcA"
                     /locus_tag="ACHLCY_001277"
     CDS             complement(46572..47285)
                     /gene="bdcA"
                     /locus_tag="ACHLCY_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0368577.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            47356..47949
                     /gene="bdcR"
                     /locus_tag="ACHLCY_001278"
     CDS             47356..47949
                     /gene="bdcR"
                     /locus_tag="ACHLCY_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0368578.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            48094..48546
                     /gene="tabA"
                     /locus_tag="ACHLCY_001279"
     CDS             48094..48546
                     /gene="tabA"
                     /locus_tag="ACHLCY_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP0368579.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            48669..>49541
                     /locus_tag="ACHLCY_001280"
     CDS             48669..>49541
                     /locus_tag="ACHLCY_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0368580.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIRDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILID"
BASE COUNT        11851 a        12315 c        13276 g        12099 t
ORIGIN      
        1 taaaccgttc gatactggat cagggctggt atgaaatgcg ccgccagctt gagtacaagc
       61 agctctggcg tggcggtcag gtgcttgctg ttccgccagc gtacacaagc cagcgttgcg
      121 cgtgctgtgg tcatacagcg aaagaaaatc gcctgtcaca aagtaaattc agatgccagg
      181 tatgtggata tacagcgaac gccgatgtaa acggcgctcg taacatttta gcggcggggc
      241 acgccgttct tgcctgtgga gagatggtgc agtcaggccg ctcgttgaag caggaaccca
      301 ccgaaatgat tcaggcgaca gcctgaacga agcagggatc cacgtccttc agggcgtgga
      361 ggatgtcaac atatcagtat ggtttccccc caggcactgg aagccagacg taaggtcatc
      421 aaagagacaa tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac
      481 tgccatccgg ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc
      541 ccgtcaccac gccgctggcg ttctggcgga ttttcaccat ttgcccgttt tgcaaattac
      601 tcagcggctt acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc
      661 cgtggtcgcg gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt
      721 gattatcaat tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgctctt
      781 ctggctggcc ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt
      841 gtggggtgac taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa
      901 tatctaactg cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca
      961 gaaatccgac caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca
     1021 gcgggtccat gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta
     1081 gttcaaagcg cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct
     1141 atgaataagc taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga
     1201 tattgctatt gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc
     1261 aaagaagtgt ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg
     1321 accaccccaa cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta
     1381 gggcaacaac tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt
     1441 attgccgatg cgctggaagg caaacatccg gctgttccgg ttgatgtggt gcatcgcgcg
     1501 ctgcgtgaaa tccacgagcg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct
     1561 gctgaaggtg tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa
     1621 tctggcctgc aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac
     1681 cgcgttcgtg ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt
     1741 gctcgtggtg aacccgctga atttccggtt aatggcgtga tccctggctg gattgaagca
     1801 ctgactctga tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat
     1861 ggcgagcgcg gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa
     1921 ctgctggaaa tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat
     1981 tcagcaataa acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa
     2041 gcgtgtattt ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat
     2101 aaaattaaca ttagatctaa atcttagtat tcatcccgcg tattgttacc taatatcgat
     2161 gagtcccgat acagattcgt cgtatcatag actgactaaa ggccgtagag cctgaacaac
     2221 acagacaggt acaggaagaa aaaaacatgg tagatcaggt aaaagtcgtt gccgatgatc
     2281 aggctccggc tgaacagtcg ctacggcgca atctcacaaa ccgacatatt cagcttattg
     2341 ccattggcgg tgccattggt acggggttgt ttatggggtc tggcaaaacg attagccttg
     2401 ccgggccgtc gatcattttc gtttatatga tcatcggttt tatgctcttt ttcgtgatgc
     2461 gggcaatggg ggaattgctg ctttcgaatc tggaatacaa atcttttagt gacttcgctt
     2521 ccgatttact cgggccgtgg gcaggatatt tcaccggctg gacttactgg ttctgctggg
     2581 ttgtaaccgg tatggcagac gtggtggcga tcacggctta tgctcagttc tggttccccg
     2641 atctctccga ctgggtcgcc tcgctggcgg tgatagtgct gctgctgacg ctcaatctcg
     2701 ccaccgtgaa aatgttcggt gagatggagt tctggtttgc gatgatcaaa atcgtcgcca
     2761 ttgtgtcgct gattgtcgtc ggcctggtta tggtggcgat gcactttcag tcaccgactg
     2821 gtgtggaagc gtcattcgcg catttgtgga atgatggcgg ctggttcccg aaaggtttaa
     2881 gtggcttctt tgccggattc cagatagcgg ttttcgcttt cgtagggatt gagctggtag
     2941 gtacaaccgc tgcggaaacc aaagatccgg agaaatcact gccgcgtgca attaactcta
     3001 ttccgatccg tatcattatg ttctacgtct tcgcgctgat tgtaattatg tccgtgacgc
     3061 cgtggagttc ggtagtcccg gagaaaagcc cgtttgttga actgttcgtg ttggtgggtt
     3121 tgcctgcggc tgccagcgtg atcaactttg tggtgctgac ctctgcggcg tcttccgcta
     3181 acagcggcgt cttctctacc agccgtatgc tgtttggcct ggcccaggaa ggtgttgcac
     3241 cgaaagcgtt cgctaaactc tctaagcgcg cagtacccgc gaaagggctg accttctcct
     3301 gcatctgtct gctcggcggc gtggtgatgc tgtatgtgaa tcccagcgtg attggcgcgt
     3361 tcacgatgat cacgacggtt tccgcgattc tgtttatgtt cgtctggacg attatccttt
     3421 gctcgtacct ggtgtatcgc aaacagcgtc ctcatctgca tgagaagtcg atctacaaga
     3481 tgccgctcgg caagctgatg tgctgggtat gtatggcgtt ctttgtgttc gtgatcgtgc
     3541 tgctgacact ggaagatgac actcgccagg cgctgctggt taccccgctg tggtttatcg
     3601 cgctggggtt gggctggctg tttattggta aaaaacgcat ggcgaagtaa tgccgctaag
     3661 gcacctgcgg gtgtctttca ggctgcaaaa ttgcctgatg cgctacgctt atcaggccta
     3721 cagaacctct gcaatatatt gagtttgcgt gcttttgtag gccgggtaag gcgtttacgc
     3781 cgcatccggc atgaacaaag cacactttgt cagcaatctc ctttgtcttt atacattcct
     3841 aaaaatactt cccgtcatcg acagctctca ggcgcgggct taccgtggat ctttgccact
     3901 cattgcagag ttcactatgg ctaagcacag ttggttatta atcgccgcat tacctctctc
     3961 catttcacct tcctggggcg cagattttta ttaccgccag caggagaaag gcacggttta
     4021 tgttgccgaa cagaagggag aaaaggatga gatcctctcc gaactgccgg gcgttaattt
     4081 ttcccgcctc tggcgtattg ccaatttagc caataaccaa gaaacccgct tactgtccga
     4141 ttttaatccc gataagttcg attgcgatga tcggaataat tgccagcata cctggctcac
     4201 cgatggacgc tctgttcttt ggtctggcaa agtcctgaaa aatccgcccg gtgaacctaa
     4261 tgtcgatgcc gccagttttc aggcattcgg tgctttcgct gccgataaac gcagcgtcta
     4321 ttttgacggt cagcgtaccg atgatagatg aagtggatat gtatatagct gtgtctcagt
     4381 tgcaaacaaa tatttatgta ccgaaataaa agtatcaata gacaattaat taataaatca
     4441 ggagataaag tggctatatt acatccacag gagtgctggt tgctggaaag aataatgtcg
     4501 ccggaatatt atcggcgtcg ttttgagggc tggcaggagt ttgtcgagct gtgcgagcga
     4561 caggtggcag agtggagcaa gaccatgccg ctggatgtgc gccgtcgtcc gctgtgcgag
     4621 cagattgacg tcgtctgggg cgggcgagtg ttgcccaata tccgcagtac tttaaagtcg
     4681 gtacagtatg actttattca gctacagcag ggggacctgc gggtactgca atccgggggc
     4741 aatattagca gtgatatgaa aggactaatt gattatccgt cggactggat gtcactcgcc
     4801 gcgcaaaaac agtatgacag actgaaatgg cgaggtgcac attataataa tttaatcaga
     4861 cgtacctcgg gcggatactg gtatgacggc gaactgactt attattatga ggaaagtctg
     4921 cacggcccgc tggccctgcc gatgcaatta ccgttgtatg agctggatag tagcgtatac
     4981 ctgcgtgagg atgactcggt aacggtggct ggcctgtatc tgccggatat cccagatgcc
     5041 agcgcccaac tgttgtaccg gagtgaacat attcccgagg cttggcaggg gcgggtacga
     5101 acgaagtatg tcaacgaggc tgggatacaa gaatattact gggaaagcgg ggcgtgggca
     5161 aaatgcaatt ggaaacgtat acggcgggtg gcaaaccgat ttatcaatgt gccgccggag
     5221 ggatttttcc cacagggcat gccggaggag ttgtacaact ggccacagcg cgaagcgcag
     5281 tatgttacag accggcagcg gatagcggcc tttagcggtg aagcttgtcc tcatagcggg
     5341 gagtggtcga tttttgtaga aggtagacaa gcgacggtga cactggagca gggagagcag
     5401 atgccagaat ggacggacag gaaaatggag ggggagtata aaagaggcga gaaattccat
     5461 gtgctgtggt cgctgatgaa ccgccatgat ggcgggtcgg tgtgggtgga agcgtagcgg
     5521 tagctggatg tacacgtaaa aaccagggaa tgggtgattt tattctggct tatacaggag
     5581 cgatgaataa gtaattatat taatgaaaaa aataagttag taaccgttga aggaacttat
     5641 tattggctga ataatgaatt ttcccagttc agtagataca aaagattgtc ccggagtggt
     5701 gaataactca aagcatttga aaacgcacct gattgtgtaa accaggtgcg ttgatcgaac
     5761 aaattactca cccgccagcg cgcgtgggaa cagtacattg ttttccaggc tgatgtgatc
     5821 catcaggtca tcaatcagtt cattaatacc gttatacatc gctttccagg tggtgcaggc
     5881 ttccggcggc ggtgtgacgt tattggtggt gtgtttaatc acttccagca gttcgcccgc
     5941 ttcatcgtgc tcgctttcca tcacgctgat tggtcccatt gcctggctgc ccatgccttg
     6001 tttgatcatc gggaagagga tctgctcttc tttcatcatg tggctggaaa gctcttcatg
     6061 cagcatagtc aggtattttg tcagcccttt tggcacgctc ggtttgtcgg cgtgaacgcg
     6121 ctcgacttta gtcgcttgca ggatcagctc cggcagctgc tcgcggtgac gatcgtggta
     6181 gcgcacgatg atatggtcaa tgatttctgc cagcggggcg ctacgccagt ctttctcaat
     6241 cggttgttca gcaagctttg ccagttcagc ttcaatgacc tcaacatcca gttctttacg
     6301 tgccgccgcg cgcgccagcg tctgcttacc gccacagcag taatccatat catatttacg
     6361 aaacagagcc gaagcgcgag ggatagagag cgccagttca cctaaaggtt ggtcgcgata
     6421 agccatagct gatacctcat tcttaataag ataagatgta ttttaaatgc atctttaagg
     6481 caaaagctat aacccttacg tagcagaggc ttatttaact cactgccagc gtgtcaactt
     6541 tctgagacag attcagttaa aggtttttct ggttttgctc tgaccgcaat gaccacgcca
     6601 atcactaaca gcgcgatacc gctcagcgtc attagcggcg gcatttgctg gcggagtaaa
     6661 aaggtgtaca gcaaacctgc cagcgtttcg aaaacaatca gcggcccgag aatcactgtc
     6721 ggtaatcgct ggctggcgac gttccagcag agtgcgccaa cccatgagca aagcacggct
     6781 atcgcaacca tcagactaat aaacaccagc ggacgggggc caaaaggtaa ggagaagtcc
     6841 ggcgtttgta tattcagcca gtaacaggcg acgagatagc cgatgagaga aaccggcagc
     6901 gtgaccagcg cctgcgccgt cgcccacatc atcggatgtt tgtcgggatt ttcccgcagc
     6961 cagcgggcgt tgcgcagggc ataccatgcc cagcagacca cggaaactaa cgctagcacg
     7021 atgccagagg tataacgtgc ccagtcaaaa tcggggagtc cgtggtttaa ctcagcaata
     7081 ttcacactcg ccaggccgat gccaatacaa atcagtgccg gggcgagttt tccccacgcg
     7141 agtttgccgt cgcgctggct ataaagcaga ttggcaaaga caggaatgac caccggcagg
     7201 gtgccgataa tcatcgtgga aacaggcgcg ccagtacgtt gaatggcact ggcaaggcag
     7261 aagtaataaa tgaggttgcc catcatagtg agcatcaagg cggtaagcca gtcccgacgc
     7321 gccaactgac gcagacgcac gcgtcccagc caggcaatgg gcagcgcaat taaccctaac
     7381 gccagataac gccccatcga ctgcaacatc gccgggtatt ccggtacgat caacggcccg
     7441 acaaaaataa gcccccacat caaccctgct aacagggcgt acagcacgcc gctaatcatt
     7501 actggcatcc attggtctgt cagaagaaag ttcaggaagc agacatgatt gctcccggca
     7561 tcccgtcatt attagagagg atgcgggagg atgccacttt aattaactag ttattaacat
     7621 taaaaagatg gcttacgctt tcggctaacg ttgtcgttgg gcggccaatc aatttgctaa
     7681 gcgttttgct gttatcaaac agaccacctt tcgatgcgcc aacgtcagaa tccgccagca
     7741 tatccgccag tccgtcgggc agtccgacgc ttttcagtgc cgcggcgaaa tcggcttcgc
     7801 tcagattttg ataggtaacc tgtttgccgc tctgtttggt cagctctgcc gctaactgtg
     7861 tcaatgtcca ggcactatcg cccgccagtt cgtaaacctt gccttcgtga ccggcttcgc
     7921 taatcacgcg tgccgcagct gccgcataat ctgcccgcgt tgctgaggcg attttgccat
     7981 cgcccgccgc accgataaat acgccgtgtt ccagtgctgc cggggcgctg gcgaggtagt
     8041 tttcgctgta ccagccgttg cgcagcaggg tgtaaacgat gccagaatca gccaacattt
     8101 tctccgtctc gatgtgctca ttggcgaggc cgagcgggga ggtatctgca tgtagcaggc
     8161 tggtataagc gataaatttc acaccagccg cctttgcggc attaataaca ttacgatgct
     8221 gcggggcacg ttgacccact tcgctggaag agatcagcag tagtttttcc actccctgaa
     8281 gtgcagatgt cagtgcggct tcatcgccgt agtcagcctg acgcacggta atgccttgtg
     8341 ctgccagagc ctgggctttt gccggattac gaacgatagc cactatttgg ctgacaggaa
     8401 ccgttttctt caaggattca ataacatagt gaccaagttg gccagtggca ccagtaatag
     8461 cgatcatggg aagtctccat cgttttgtct tgtgtttaaa tacgctaaca cctaaactta
     8521 cttttagtaa gtacgcacaa aaaggtaagt atgaaatgag tcaggttagc ctgtcgcaac
     8581 aactgaaaga gggtaatctc tttgcggaac agtgcccgtc gcgcgaggtg ttgaaacacg
     8641 tcaccagccg ttggggggtg ttgattctgg tggcgctacg cgaaggtact catcgcttta
     8701 gcgacctgcg gcgcaaaatc ggcggggtga gcgaaaagat gcttgcgcag tcgttacagg
     8761 cgttagaaca ggatggtttt cttaaccgta tcgcgtatcc ggtggtgccg ccgcatgtgg
     8821 aatatagcct cacgccgctg ggcgagcagg tgagcgaaaa ggttgctgca ctggcggact
     8881 ggattgagtt gaatttgccc gaggtgttgg cggtgcggga tgaacgtgcg gcataactgc
     8941 cggatgcgct gagcttatct ggtctacggt gcatggcttg tagggcggat aagatgcgcc
     9001 agcatcgcat ccgccaataa atgccggatg cggttgatga tttataggcc gaaaaagaag
     9061 tgttttactt actcaaatcc acctgataaa tcgcaaaccc gatatcatcg gtcgcgactt
     9121 tattcatcgg atattgccct ttctctttaa taaacgctgc ggctttatct gacggagaag
     9181 tttcgaaacg gatatccagt ttcttatcgc cagctatcgg tgctaaacgc cagttgttat
     9241 ctgccgccgg gtgaatttcc cccgcacgtt tcgactcatc agcaatccac gctgccagca
     9301 ccgagcggtt ctcatccggt gaagcaaaag cgatatggct gtcgcccgta ccggcaaatt
     9361 tgccgccgta agcgcgatag ttattggtgg caacgaggaa catggcgttc ggatcaatcg
     9421 gcttgccatt aaaggtcagg ttcttaatcc tttccgcatt ggcattaatc atctggcact
     9481 cgccgtcata acgggcaggt tgggtaacat caatctgata attcacacca tcaatcacat
     9541 caaagttata ggtgcggaaa ccatcccagt tgatgagtga ttgcggtttc gtgctgttag
     9601 gatcaatctg gttaaactgt ccggcggagc actccagcca ctctttcacc tctttaccgc
     9661 tggctttcac cacaatcagc gtattggggt agagataaag atcggcggca ttacggaagg
     9721 tcaactggcc tttttccacc tccacatagc ttgccgggtc atttttgcga ccaccaactt
     9781 taaacggtgc ggcagctgaa agcaccggca gttttgccag atccggatcg ccctgaatgt
     9841 aatgttcgac atacgctttt tgcgcgttgt tcactacttg cacggtcgga tcgtcctgca
     9901 ccagcgccag atagctatac atattgtcgg cagatttacc gattggcttg ctgacgaact
     9961 ggcgtgtggc atcgtgatcg gctttgagtg tttctaccag cttgctgtct tccgccgcga
    10021 gggatttttt attggcgatg tcgtaaatcg gccgagcttc cgctttcgcc tgcgtcacct
    10081 gccatttacc gctgtcatta ctgagttgta agtcgaccac cccaagatga tcgccccaca
    10141 tgcccggcat taccgccgga acaccattca gcgtgccttt ggtgatatca gccccttcga
    10201 tatcagcaaa atctttaccc gggaaaacgg cgtgagcatg accaaacata atggcgttaa
    10261 cgcccggaat ttcactgagg taataaactg agttttccgc cattacttta tacggatcgg
    10321 cagacagccc ggaatgcgcc agaacgacaa cgacatcggc acctttctcg cgcatttcag
    10381 gcacgtattt gcgcacggtt tcggtaatat cattcaccgt cacttttccg gataaattag
    10441 ctttatccca gcccatgatt tgcggcggca cgacgccaat atagccaatc ttcagcgtct
    10501 gtttttttcc gtctttatcg accacttcgg tatctttaat taaatacggt gtaaacattg
    10561 gctgtttggt tctggcgtca atgacgttgg catttacata agggaatttc gctcccgcca
    10621 gcgcattttt caggtaatcc agaccgtagt taaattcatg attgccgagt gtaccgaccg
    10681 tataatccag cgtattcagc gccttataaa ccggatgaac atcacctgct tttaatcctt
    10741 tcgccgacat gtaatcggcc agcggactcc cctgaatcaa atcgccgtta tcgaccagta
    10801 cgctgttttt cacttcattg cgggcatcgt taatcaggct tgccgtacgt accagtccga
    10861 atttttccgt ggcggtgtct ttgtaataat cgaaatccat catgttgcta tgcagatcag
    10921 tggtttccat gatacgcaga tcgaccgtcg ctgcattcac actggcggca atcagcgtgg
    10981 ccaggagcgt tgcgctaaac ttaatcatca gggacatcct tttatcatcg ggaatacgaa
    11041 agaaaaggaa gaataaacgt cttacttata gaacagtgaa gaatgccaca attttacgct
    11101 ttgaaaatga tgacactatc acagttggcg cattcattaa cgatagggta taagtaaaac
    11161 aataagttaa caccgctcac agagacgagg tggagaaatg ttagatcaag tatgccagct
    11221 tgcacggaat gcaggcgatg ccattatgca ggtctacgac gggacgaaac cgatggacgt
    11281 cgtcagcaaa gcggacaatt ctccggtaac ggcagcggat attgccgctc acaccgttat
    11341 catggacggt ttacgtacgc tgacaccgga tattccggtc ctttctgaag aagatcctcc
    11401 cggttgggaa gtccgtcagc actggcagcg ttactggctg gtagacccgc tggatggtac
    11461 taaagagttt attaaacgca atggcgaatt caccgttaac attgcgctca ttgaccatgg
    11521 caaaccgatt ttaggtgtgg tgtatgcgcc ggtaatgaac gtaatgtaca gcgcggcaga
    11581 aggcaaggcc tggaaagaag agtgcggtgt gcgcaagcag attcaggtcc gcgatgcgcg
    11641 cccgccgctg gtggttatca gccgttccca tgcagatgcg gagctgaaag agtatctgca
    11701 acagcttggc gaacatcaga ccacgtccat cggctcttcg ctgaaattct gcctggtggc
    11761 ggaaggacag gcgcagctgt acccgcgctt cggaccaacg aatatttggg acaccgccgc
    11821 tggacatgct gtggccgcag ctgccggagc gcacgttcac gactggcagg gtaaaccgct
    11881 ggattacact ccgcgcgagt cgttcctgaa tcctgggttc agagtgtcta tttattaatt
    11941 aatttatgca gcaggtccat cacttgctgc acctcttcct gagtaagcgc cctgtctttg
    12001 gcccattgca cgcgaccgtc tttatccagc accactacag cggaactttt ttcatccagc
    12061 tgccaggcac cgcgtgcgac gccattgcta tcaacaataa actgcgacca gggataaagc
    12121 tttttattac tctccagact gctgcgcaca aacatgccgg aacccggaat tgcgtcgtcg
    12181 gtgttaacaa tggtggtggt ctggtaacga tcgtgcggta acttcgctga tttaatcgct
    12241 tcaatcagcg tcgcgttttt ctcttttgca gaggtgcgac cagcaatatg ttgcagtact
    12301 cgcacttttc ccactaactg cgcgctgttc caggttttgt agctaaactg atctttatca
    12361 agcaccaact cgccacgatc ggtgatgcca atcggcggca ctcgctgacc ggtttcgaac
    12421 tgatgtgcgg aagccatcat cggcaacagc aggcaggtga gtgccagaat cttgcgtagg
    12481 gtcatggtgt ttccttctta tgatatgcag gtgatccgac cacttgggtc tgagttttaa
    12541 tcatatgtgc tatttatcga atttcccgca agtgtgatgc cagtttgcgg tcaagcgcac
    12601 aaatcatatg aaaaatgaat gcttatactg aagaccgcgc ttcggtaaaa agataattct
    12661 gaataattgt aacctttagg taaaaaaagt tatacgcggt ggaaacattg cccggatagt
    12721 ctatagtcac taggcattaa aatttgcgcc tcataatagt tgggccgatt gtggcaccgc
    12781 acaggcgtaa tactcagcag gagataacaa tgaaaatttt ccaacggtac aacccacttc
    12841 aggtggcgaa gtacgtaaag atcctgttcc gtggacggtt atacatcaag gacgttggcg
    12901 cttttgaatt tgataagggt aagattctta tcccaaaagt gaaggataag ctgcatttgt
    12961 cagtgatgtc cgaagttaac cgtcaggtta tgcgtctgca aacagagatg gcttaaccaa
    13021 agtgctatgc agtaataaga cggctcctga ttcaggagcc gttgatgttt ctgggggtta
    13081 cgcgacgctt tcttctttat ctttagcgtc aggcagtttt ggcgaaaggg cggtggcctt
    13141 gctgtcgatc cgggtcacca gcagctggtc gatgcggtag ttatcgatat ccaccacttc
    13201 aaatttgtag ccggcgaatt tcaccgaatc ggtgcgtttc gggatcttac gcagcataaa
    13261 catcataaag cctccgatgg tttcgtagtt gcccgactgc gggaactcgt caatatccag
    13321 cacgcgcatg acgtcgtcaa ttggggtgcc gccgtcaatc agccatgagt tctcatcacg
    13381 ggcgacaatc tgctcttcca gcccctgacc gaccagatcg cccatcagcg tggtcatcac
    13441 gtcgttgagg gtgatgatcc ccaccaccag cgcgtactcg ttcatgatca ccgcgaagtc
    13501 ttcacctgcg gttttaaaac tttccaacgc ctctgaaagg gttaacgtat ccggcacaat
    13561 cagcgtgttg cgaatttgta cgccgctgtt cagtgccagg ctttggttag ccagcacgcg
    13621 gttcagcagg tctttagaat cgacataacc gatgatgtgg tcaatatctt cattacagac
    13681 gaggaactta gagtgcggat gttccgccac cttattcttc aggctttgct catcttcgtg
    13741 gagatcaaac caaatcacgt tttcacgcgg tgtcattgaa gacggaacgg tacgggattc
    13801 cagctcaaag acgttttcaa tcagctcgtg ttcctgttta cgtaacacgc ccgccagcgc
    13861 accggcttcc actaccgcgt agatgtcatc agaagtgatg tcatctttac gtaccattgg
    13921 cagtttgaaa atacggaaga tcatgttcgc caggccgttg aagaaccaca ccagcggggt
    13981 gcaaacgtac aggcagaagc gcatcgggtt gatgatacgc aaagccaccg cttctggcgc
    14041 aatcataccg atgcgtttcg gggttaaatc cgcaaacagg ataaacatgc cagtcactaa
    14101 cgagaaagag agaataaagc tcagttgctc agagagttct gccgacatat agcgggagaa
    14161 caggctgtga aaagctggag aaaatgccgc atcaccgacg ataccgccga gaatcgcaac
    14221 tgcgttcaga ccgatttgga ccaccgtaaa gaacatgccg ggattttcct gcatattcag
    14281 aacgcgttgg gcatttatat tgccttcatc agccagcagt ttaagtttga ttttgcgtga
    14341 ggcggcaagt gagatctcag acatcgagaa gaacgcactt acagcgatca agcagagtat
    14401 gactaaaata ctgtttaaca tatcttatcc gacctttcag gccagatcct cggaagggga
    14461 agttgattaa tttgtgtgaa atacacattg aaagccgatt cgaagaaagt gatccggcat
    14521 tttcagtggc tagtatagcg taaggtactg taaagtcgcc agagggttaa aattcagact
    14581 gctgataaat gcaatactgc ctgatgcgct atgccgatca ggcctacgca gtccctgcaa
    14641 tatattgaat ttggacgatt ttgtaggcct ggtaaggcat tcacgctgca ttcggcataa
    14701 acaaaacgca ctttatcaac aatctacatt tgtacccgta acgctatgct tccggtggca
    14761 gacagacgcc aattccgcca attccacagt aaccatacgg gtttttatgc agatattgct
    14821 ggtggtcatc ttcggcataa taaaacggtg tggcgttagc gatttccgtg gtgatgtgac
    14881 gatcgtcatc ggcggcaagc atcgccgcct gaaaacgttc cagactggcg cgagctgcgg
    14941 catcctgttc tggggtcagc ggataaatcg ctgaacgata ctgcgtgccg tggtcattgc
    15001 cctgacgcat tccctgggcg ggatcgtgat tctcccaaaa tacctgtagc aactgctcat
    15061 agctgatgac agaaggatcg taaacaatgc gtaccgcttc ggcatgaccc gtatcaccgg
    15121 agcacacttc ccgataagtc ggatttggcg tatagccgcc ggtatagcct gcggcggtgc
    15181 tgtaaacgcc gggtaactgc cagaacagac gctccacacc ccagaaacaa cccatcgcaa
    15241 aaatggcaat ctccattccg tcaggtacat tggtcattga gtgaccgttg accgcatgca
    15301 gcgtggctac gggcatcggg gtgttacgtc caggcagggc atcggcgggg gaaaccagat
    15361 gctttttatc aaataaactc atggtgtcgc tctcccgaaa atcggtcatt ggggttaagg
    15421 ttgtaacaag aggcgtattt gcacacaata accaccgtga ataggtctaa agtaaaacat
    15481 aagaaatatt tggggtttag tctgcttttt aatccatatt actggatttt tgttaagccg
    15541 tttaacggcg ttccaggggc aggaaaaaag gatattcagg agaaaatgtg cgctatatcc
    15601 gacagttatg ctgtgtaagc ttactctgct taagcggatc tgccgtcgcc gcgaacgtcc
    15661 gtctacaggt cgaggggtta tcgggacagc tggaaaagaa cgttcgtgcg cagctttcta
    15721 cgattgaaag tgatgaagtg acgccagacc gtcgctttcg cgcacgcgtc gatgatgcca
    15781 tccgcgaagg tctgaaagcg ctgggttatt accagccgac cattgaattt gatctccgtc
    15841 caccgccaaa gaaagggcgg caggtattga tcgccaaagt cacgccaggc gtgccggtgt
    15901 taattggcgg caccgatgtg gtattgcgcg gcggcgcgcg gaccgataaa gactatttga
    15961 aattgctcga tactcgcccg gctattggca cggtactgaa ccagggcgat tatgaaaatt
    16021 tcaaaaagtc cttaaccagc attgcgttgc gtaaaggtta tttcgatagc gaatttacca
    16081 aagcgcagct gggcattgcg ctcggcctgc ataaagcctt ctgggatatt gattataaca
    16141 gtggcgaacg ttaccgcttt gggcatgtga cctttgaagg atcacaaatc cgcgatgaat
    16201 acctgcaaaa tctggtgccg tttaaagagg gcgatgagta cgaatcgaaa gatctggcgg
    16261 aacttaaccg tcgactttct gctaccggct ggtttaactc ggtcgtagtg gctccacaat
    16321 ttgataaagc gcgcgaaacg aaagtattac ccttgacggg cgtggtttcg ccgcgaactg
    16381 aaaacaccat cgaaaccggg gtcggttact ctacggacgt gggaccgcgc gtgaaagcta
    16441 cgtggaaaaa gccgtggatg aactcttatg gtcacagtct gaccaccagt accagtattt
    16501 ccgcgccgga acagaccctc gacttcagct ataaaatgcc gctgctgaag aatccactgg
    16561 aacaatatta tttggtgcag ggcggtttta agcgcactga cctgaacgat accgaatctg
    16621 actccactac gctggtggct tctcgctact gggatctctc cagcggctgg cagcgtgcca
    16681 ttaacctgcg ctggagtctc gaccacttta ctcagggtga aattaccaac accacgatgc
    16741 tgttttatcc tggggtgatg attagccgca cgcgttctcg tggtggcctg atgccaacca
    16801 ggggcgactc gcaacgctac tctatcgact actccaacac ggcctggggc tcagatgtcg
    16861 atttctccgt tttccaggcg cagaacgtct ggatccgcac actgtacgat cgccatcgtt
    16921 tcgttacacg cggcacgctg ggctggattg aaaccggtga tttcgacaaa gtaccgccgg
    16981 atctgcgttt tttcgccggg ggcgatcgca gtattcgcgg ctacaaatac aaatctatcg
    17041 ccccgaaata cgccaacggt gacctgaaag gggcctcgaa gttgataacc ggatcgctgg
    17101 aataccagta caacgtgacc ggaaaatggt ggggcgcggt gtttgtcgat agtggcgaag
    17161 cggtaagcga tattcgccgc agcgacttta aaaccggtac cggggtcggc gtgcgctggg
    17221 aatcgccggt cgggccaatc aaactcgatt ttgccgtacc ggtcgcggat aaagacgaac
    17281 acgggttaca gttttacatc ggtctggggc cagaattatg agtttatgga aaaaaatcag
    17341 cctcggcgtg gttatcgtta tcttactgtt gctgggatcg gtggcgtttc tggtgggcac
    17401 caccagcggc ctgcatcttg tatttaaagc ggcggatcgc tgggtgccag gactggatat
    17461 tggcaaggtc accggcggct ggcgcgatct caccttgtct gacgttcgtt atgagcagcc
    17521 aggcgtggcg gtaaaagcgg gtaatctgca tctggcggtc ggtcttgagt gcctgtggaa
    17581 cagtagcgtt tgtattaatg acctggcgct gaaagacatt caggtcaaca tcgacagtaa
    17641 aaaaatgcct ccttctgaac aggttgaaga agaggaagat agcggtccgc tggatctctc
    17701 cacgccgtat cccatcaccc tgacacgggt ggcgctggac aacgtcaaca tcaagattga
    17761 tgacaccacg gtgtcggtga tggacttcac ctccggcctg aactggcagg agaaaaccct
    17821 gaccctgaaa ccgacgtcgc tgaaaggcct gctgattgct ctgccgaaag tggcggaagt
    17881 ggcgcaggaa gaagtggtcg aaccgaaaat tgaaaatccg cagccggagg aaaagccgct
    17941 cggcgaaacg ctgaaagatc tcttttctcg cccggtattg ccggaaatga ccgacttgca
    18001 tttgccgctt aacctgaaca ttgaagagtt taaaggcgag cagttacgcg tgacgggcga
    18061 cacggacatc accgtgcgca ccatgctgct gaaagtgagc agcattgacg gcaatactaa
    18121 actggacgcc ctggatatcg attccagtca agggatcgtc aacgccagcg gcacggcgca
    18181 gctgtcagac aactggccgg tggatatcac cctcaacagc acactgaacg tggagccgtt
    18241 gaaaggtgaa aaagtgaagc taaaagtggg cggcgcgctg cgcgaacagc tggagattgg
    18301 cgttaatctt tccggtccgg tggatatgga tttacgcgcc cagacgcgac tggcggaagc
    18361 cggattgcca ctcaacgtgg aagtgaacag caaacagctt tactggccgt tcaccggtga
    18421 gaagcagtat caggcggatg atctgaaact gaaacttacc ggcaaaatga ccgattacac
    18481 gctctctatg cgtacggcag tgaagggact ggagatcccg ccagccacca ttacccttga
    18541 tgccaaaggt aatgaacagc aggtcaatct cgacaaactc accgtcgcgg cgctggaagg
    18601 gaaaactgaa ctcaaggcgt tgctcgactg gcagcaggcc attagttggc gcggtgagct
    18661 aacgcttaac ggcattaaca ccgccaaaga gatcccagag tggccgtcga aactcaatgg
    18721 cttgattaaa acccgcggta gcctgtacgg cggcacttgg cagatggagg tgccagaact
    18781 gaagctgacc ggtaacgtca aacagaacaa agtgaacgtt gacggcacgc tgaaaggcaa
    18841 cagttatatg cagtggatga tcccagggct tcatctggaa ctggggccaa acagtgccga
    18901 agtgaaaggc gagctggggg taaaagatct caatcttgat gccaccatca acgcgccggg
    18961 gctggataac gcgctgccgg ggcttggcgg tacagcgaaa gggctggtga aagttcgcgg
    19021 cacggtggaa gcgccacaac tactggcgga catcaccgcg cgcggcctgc gctggcagga
    19081 gctaaccgtg gcgcaggttc gcgtggaagg cgatatcaaa tccaccgatc agattgcggg
    19141 taaactcgac gtgcgcgttg agcaaatttc acagccggat gtaaatatca acctcgtcac
    19201 cctgaatgcc aaaggcagcg aaaaacagca cgagttacag ttgcggattc agggcgagcc
    19261 ggtttccggg cagcttaatc tggcaggaag ttttgatcgc aaagaagaac gctggaaggg
    19321 aactcttagc aatacccgct tccagacgcc ggtcggcccg tggtcgctga cccgcgatat
    19381 tgcgctggat taccgcaata aggagcaaaa aatcagcatc gggccacact gctggcttaa
    19441 cccgaatgcg gaactgtgcg tgccgcaaac tatcgatgcg ggggccgaag ggcgtgcggt
    19501 ggtgaatctc aaccgcttcg acctcgccat gctgaaaccg tttatgccag aaaccactca
    19561 ggccagcggt atcttcacgg gtaaagcgga tgttgcctgg gacaccacga aagaggggct
    19621 gccgcagggc agtatcaccc tttcggggcg taacgtgcag gtaacgcaaa ccgtcaacga
    19681 tgcggcgctg cccgtggcgt ttcagacact gaatctgacg gcggaattgc gtaacaaccg
    19741 tgccgaattg ggctggacca tccgcctgac caataacggc cagtttgatg gacaggtgca
    19801 ggtgaccgat ccgcaaggcc gccgtaatct tggtggcaac gtcaatatcc gtaacttcaa
    19861 ccttgcgatg ataaacccca tctttaaccg tggggaaaaa gcagcgggga tggtgagtgc
    19921 caacttgcgt ctgggtggtg atgtgcaaag cccgcagttg tttggtcagc ttcaggttac
    19981 gggtgtggat atcgacggca actttatgcc gtttgatatg caaccgagcc agcttgcggt
    20041 caactttaat ggtatgcgct cgacgcttgc cggtacagta cggacccagc agggtgaaat
    20101 ctacctgaac ggtgatgccg actggagcca aattgaaaac tggcgggcgc gggtaacggc
    20161 gaaaggcagt aaagtgcgga tcaccgtgcc gccgatggta cgaatggatg tatcgccaga
    20221 tgttgtcttc gaggctacac caaacctgtt taccctcgat ggtcgcgtgg atgtcccgtg
    20281 ggcgcgcatc gtggtgcacg atctgccgga aagcgcagta ggcgtctcca gcgatgtggt
    20341 gatgcttaac gataacctgc aacctgaaga gccgaaaacg gcgtcgattc cgattaacag
    20401 taacctgatt gtccacgttg gcaacaatgt gcgcattgac gcctttggcc tgaaagcgcg
    20461 gctgacgggc gatcttaacg tcgttcagga caaacaaggg ctgggcctga acgggcagat
    20521 caacatccct gaagggcgct tccatgccta tggtcaggat ctgattgtgc gtaaaggtga
    20581 gttactgttc tctggtccgc cggatcaacc gtatcttaat attgaagcta ttcgtaaccc
    20641 ggatgctaca gaagacgacg taatcgccgg agttcgcgtc actggtctgg cggacgaacc
    20701 gaaagcggag atcttctctg acccggcgat gtcgcaacaa gctgcattgt cttatttgct
    20761 acgtggacaa gggctggaga gcgatcagag cgacagtgcg gcaatgacct cgatgctgat
    20821 tggtctgggg gttgcgcaaa gtggccagat tgtgggtaaa atcggcgaga cgtttggcgt
    20881 aagcaattta gcgctcgaca cccagggagt aggcgactcc tcccaggtag tggtcagcgg
    20941 ctatgtattg ccaggtctgc aagtgaaata tggcgtgggt atatttgact ctatagcaac
    21001 actcacgtta cgttatcgcc tgatgcctaa gctatatctg gaagccgtgt ctggtgtaga
    21061 ccaggcactg gatttgctct atcagttcga gttttagcaa tgcgaatatt tgtctacggc
    21121 agtttacgcc acaaacaagg caacagtcac tggatgacca atgcccagtt actgggcgat
    21181 ttcagtatcg ataactacca gttgtatagc ctgggccact atccaggcgc agttccgggg
    21241 aacggaacag tacacggtga agtttatcgt attgacaacg ccacgctggc cgaacttgat
    21301 gccttgcgca ccaggggcgg tgaatacgcg cgccagttga ttcagacgcc gtacgggagt
    21361 gcatggatgt acgtttatca acgacccgtc gatggattaa agctaattga aagcggcgac
    21421 tggttagaca gggataagta accatatgca tacgccacct tcgggtggcg ttgttttttg
    21481 cgagacgact cgcattctgt tttgtaattc cctcaccctt tgcttttctc tccgagccgc
    21541 tttccatatc tgttaacgca taaaaaactc tgctggcgtt cacaaatgcg caggggtaaa
    21601 acgtttcctg tagcaccgtg agttatactt tgtataactt aaggaggtgc agatgcgtat
    21661 taccataaaa agatggggga acagtgcagg tatggtcatt cccaatatcg taatgaaaga
    21721 acttaactta cagccggggc agagcgtgga agcgcaggtg agcaacaacc aactgattct
    21781 gacacccatc tccaggcgct actcgcttga tgaactgctg gcacagtgtg acatgaacgc
    21841 cgcggaactt agcgagcagg atgtctgggg taaatccacc cctgcgggtg acgaaatatg
    21901 gtaaagaaaa gtgaatttga acggggagac attgtgctgg ttggctttga tccagcaagc
    21961 ggccatgaac agcaaggtgc tggtcgacct gcgcttgtgc tctccgttca agcctttaat
    22021 caactgggaa tgacgctggt ggcccccatt acgcagggcg gaaattttgc ccgttatgcc
    22081 ggatttagcg ttcctttaca ttgcgaagaa ggcgatgtgc acggcgtggt gctggtgaat
    22141 caggtgcgga tgatggatct acgcgcccgg ctggcaaagc gtattggtct ggctgcggat
    22201 gaggtggtgg aagaggcgtt attacgcttg caggcggtgg tggaataagg tgtgtttatt
    22261 tatcgcgggc ataaaaaaac ccttactaac cgaagctcgg cgttcagggt tattgcgcta
    22321 gaagaactta tttattcttt gcgcgctcga aggaagcaac gatttcagct ttagcggctt
    22381 ctgcgttttc ccaaccttca actttcaccc acttgccttt ttcgaggtct ttgtagtgct
    22441 cgaagaagtg agcgatctgc gctttcagca gctctggcag atcgttaacg tctttaatgt
    22501 gatcgtattc tttgctcagc ttgctgtgcg gaaccgcaac cagtttcgca tcttcaccgg
    22561 cttcgtcggt cattttcaga acgccaaccg gacggcaacg gatcacagaa cccggctgca
    22621 gcgggtacgg agtcgggacc agtacgtcaa ccgggtcacc gtccagagac agggtgtggt
    22681 tgatgtaacc gtagttgcac ggatagaaca tcgcggtgga catgaagcgg tcaacgaaca
    22741 gtgcgccgct ctctttgtcg atttcgtatt tgatcggatc tgcgttagcc gggatctcaa
    22801 taacaacgta gatgtcttcc ggcagatctt tacccgcagg gacgttgagt aagctcatgt
    22861 ctgtttcctt taaaaatatg tggcaaacaa gtgccgagta ttatagccaa ctcgcgccga
    22921 atgtctttgc ttgttttcgt cttcattttc ctttttcgcc agttttaaga cggtatacag
    22981 agcaggaaaa tccataaccg tagccgcatt tttcatagtg agatgaaagc gattacaaac
    23041 ttgtgattaa cgttttattt acttttttga agtgtgatgt aacgcaatcc gttacataac
    23101 gaattgtcta tagttttttc gcgaacatct tttcaccaat aataattacc ccgacgagga
    23161 caaccctatg tggaaacgct tacttgtagt ctctgcagtc tcggcagcca tgtcgtctat
    23221 ggcgttggcc gctccattaa ccgtaggatt ttcgcaggtc ggatcggaat ccggctggcg
    23281 cgctgcagaa accaatgtgg cgaaaagtga ggccgaaaaa cgcggaatta cgttgaaaat
    23341 tgccgatggt cagcaaaagc aggaaaacca gattaaagcg gtacgttcat tcgttgcaca
    23401 aggggtggat gcgatcttta tcgctccagt ggttgcgacg ggttgggaac cggtattaaa
    23461 agaggcgaaa gatgccgaaa tcccggtctt cttgcttgac cgttccatcg atgtgaaaga
    23521 caaatctctc tatatgacca ccgtcactgc cgacaacatc ctcgaaggca agttgattgg
    23581 tgactggctg gtaaaagaag tgaatggcaa accatgcaac gtggtggaac tgcagggcac
    23641 cgttggggcc agcgtcgcca ttgaccgtaa gaaaggcttt gccgaagcca ttaagaatgc
    23701 gccaaatatc aaaatcatcc gctcgcagtc aggtgacttc acccgcagta aaggcaaaga
    23761 agttatggag agctttatca aagcggaaaa caacggcaaa aacatctgca tggtttacgc
    23821 ccataacgat gacatggtga ttggtgcaat tcaggcaatt aaagaagcgg gcctgaaacc
    23881 gggcaaagat atcctcacgg gttccattga cggcgtaccg gatatctata aagcgatgat
    23941 tgatggcgaa gcgaacgcca gcgttgaact gacgccgaac atggcaggcc ccgcttttga
    24001 cgcgctggag aaatacaaaa aagacggcac catgcctgaa aagctgaccc tgaccaaatc
    24061 caccctttat ctgcctgata ccgcaaaaga agagttagag aagaagaaaa atatggggta
    24121 ttgagggttg ctatgcctga tgccgattcg taggccggat aaggcgctcg cgccgcatcc
    24181 ggcgatggtg cactgaagcc tgatgcgacg cttaccgcgt cttatcatgc ctactgggag
    24241 cacgctttac accgggggaa accatgacga ccgaccaaca ccaggagatc ctccgcaccg
    24301 aaggattaag taaatttttc cccggcgtca aagcgttaga caacgttgat ttcagcctgc
    24361 gccgtggcga aatcatggcg ctgcttggtg aaaacggggc gggaaaatca acgctaatca
    24421 aagcattaac tggtgtatac cacgccgatc gcggcaccat ctggctggaa ggccaggcta
    24481 tctcaccgaa aaataccgcc catgcacaac aactcggtat cggcaccgtc tatcaggaag
    24541 tcaacctgct acccaatatg tcggttgctg ataatctatt tataggccgc gaacccaaac
    24601 gtttcggcct tctacgccgt aaagagatgg aaaagcgcgc caccgaactg atggcatctt
    24661 acggtttctc cctcgacgtg cgcgaaccgc ttaaccgttt ttcggtggcg atgcagcaaa
    24721 tcgtcgccat ttgccgggct atcgatctct ccgccaaagt gctgatcctc gatgaaccca
    24781 ccgccagcct cgacacccag gaagtggagc tattatttgg cctgatgcgt cagttgcgcg
    24841 atcgcggcgt cagcctgatc tttgtcactc actttctcga tcaggtctat caggtcagcg
    24901 atcggatcac cgtcttacgc aacggcagtt tcgtaggctg tcgggaaacg cgcgagctac
    24961 cgcagatcga actggtaaaa atgatgctgg ggcgcgagct ggatacccac gcgctacagc
    25021 gtgccgggcg aacattgttg agcgacaaac ccgttgccgc gttcaaaaat tacggcaaaa
    25081 aaggaacgat agcaccgttt gatctcgaag tgcgccccgg cgagatcgtc ggtctggctg
    25141 gcttgctggg atccggacgt accgaaaccg ccgaagtgat cttcggtatc aaacctgctg
    25201 acagcggcac ggcgttgatc aaaggcaaac cgcaaaccct gcgatcgcca catcaggctt
    25261 cagtactggg catcggattc tgcccggaag acaggaaaac cgatggcatc atcgctgccg
    25321 cctcggtgcg ggaaaatatc atcctcgctc tccaggccca gcgcggctgg ctacgaccca
    25381 tttcccgcaa agagcagcaa gagattgccg aacgctttat ccgccagctt ggcattcgca
    25441 caccttcaac tgaacaaccg attgaatttc tctctggcgg caatcagcaa aaagtgttgc
    25501 tttcacgttg gctactgacc cgaccgcaat ttctgatcct cgacgagcca acccgcggca
    25561 ttgatgttgg tgcccacgcc gagatcatcc gcctgattga aacgctgtgt gctgacggtc
    25621 cggcgctgtt ggtgatctcc tccgaactgg aagagctggt gggctatgcc gatcgggtga
    25681 ttatcatgcg cgatcgcaaa caggtggcgg agatcccgct ggcagagctt tccgttccgg
    25741 cgatcatgaa cgccattgcg gcgtaaggag aacagtgtga tgcctcaatc tctcccggac
    25801 actacgccgc cgaaaaggcg ctttcgctgg ccaacgggaa tgccgcagct ggcggcgcta
    25861 ttgctggtgc tgctggtcga tagcctggta gccccgcatt tctggcaggt ggtgcttcag
    25921 gatgggcgtt tgttcggtag ccctatcgac attcttaacc gtgcagctcc cgttgcgtta
    25981 ctggcgattg gtatgacgct ggtgatcgcc accggtggga ttgatctctc cgtaggggcg
    26041 gtgatggcta tcgccggagc cacaacggct gcgatgacgg tcgcgggatt cagcctgccg
    26101 attgttttgt taagcgccct gggcaccggc atcctggcgg gattgtggaa cggcatactg
    26161 gtagcgatcc tcaaaattca gccgtttgtc gccaccctga tcctgatggt cgccgggcgc
    26221 ggcgtggcgc aactgatcac cgccgggcag atcgtcacgt ttaactcgcc ggatctctca
    26281 tggtttggca gcggatcgct gttgttcctg ccaacgccgg tcattatcgc ggtgctgacg
    26341 cttctcctgt tctggctgtt gacccgcaaa acggcgctgg ggatgtttat cgaagccgtt
    26401 ggtatcaaca ttcgggcggc aaaaaatgcc ggggtaaaca cgcggatcat cgtcatgctc
    26461 acctacgtgt tgagcgggct gtgtgcggcg attgcgggca ttatcgtggc ggcggatatt
    26521 cgcggtgccg atgccaataa cgccgggtta tggctggagc tggacgccat tcttgcggtg
    26581 gtgattggcg gcggatcgct gatgggcggg cgctttaacc tgctgctctc agtcgtgggg
    26641 gcgctgatta ttcaggggat gaacaccgga attttacttt ctggctttcc gccggagatg
    26701 aaccaggtcg taaaagcggt ggtggtgctt tgtgtgctga ttgttcagtc gcaacgcttt
    26761 atcagtctga ttaaaggagt acgtagccat gataaaacgt aacttgccgc tgatgatcac
    26821 catcggcgtc tttgtgctgg gctatctcta ctgcctgacc cagtttcccg gttttgcttc
    26881 cacaagagtg atctgcaata tcctgaccga taacgccttt cttgggataa tcgccgttgg
    26941 catgaccttt gtgatcctct ctggcggtat cgatctctcc gtcggttcgg tgatcgcctt
    27001 tactggcgtg tttctggcaa aagtgattgg cgatttcggc ctctcgccgc tgctggcgtt
    27061 tccgctagtg ctggtgatgg gctgtgcctt tggcgcattt atggggcttt tgatcgacgc
    27121 cctgaagatc ccggcattta tcattaccct ggcaggaatg ttctttttgc gcggcgtcag
    27181 ctatctcgtt tcagaagagt cgatcccgat aaaccatccc atttatgaca cgctctcaag
    27241 ccttgcgtgg aaaatccctg gcggcggtcg cttaagtgcg atgggactgc tgatgctggc
    27301 ggtggtggtt atcggcatat tcctcgcgca tcgtacccgt tttggtaatc aggtatacgc
    27361 cattggcggc aacgcaacgt cggcaaacct gatggggatt tctacccgca gcaccactat
    27421 tcgcatttat atgctctcca ccggactggc aacgctggcg gggattgtct tctcgattta
    27481 tacccaggcc ggatatgcgc tggcgggcgt aggtgtggaa ctggatgcta tcgcctcagt
    27541 ggtaattggc ggtacgcttt tgagcggtgg cgttggaacg gtattaggga cgctttttgg
    27601 cgtggcgatt caggggctaa ttcagactta tatcaacttt gacggtacgc tgagttcctg
    27661 gtggacgaaa atcgccatcg gcattttgct gttcattttt attgccttac agcgtggatt
    27721 aacggtgctg tgggagaatc gtcagagttc gccagtgaca agagtcaata ttgcgcagcg
    27781 ataaaaacgc ctctccgtgt ggagaggcgc aggagattac gcgtccggga attcacggat
    27841 aaagcgttcg acatcttcaa ccatatggtc gttgccgaca aagaacgaac ggcgctggtg
    27901 cagagtttcc gggatgatat ccagaatacg ctctttgcca tcgctcgctt taccgcccgc
    27961 ttgttccgcc aggaatgcca tcgggttgca ctcatacagc aaacgcagtt tgccgtccgg
    28021 gtggctggcg gtgcttgggt agagataaat accgcctttc agcaggttac ggtggaaatc
    28081 cgcgaccagt gaaccgatat aacgtgaggt atacgggcgg ttggtggatt tatcttcttc
    28141 ctggcagaat ttaatgtact tcttcacccc gttcggaaac ttaatgtagt ttccttcgtt
    28201 gatggagtag gttttgcctt tctccgggaa gcgcatccgt tcctggcaca ggcagaaaac
    28261 gcccagcgaa ggatcgtaag taaaggcgtg aacaccgcat ccggtggtgt aaaccagcat
    28321 ggtagaggag ccgtatacca cgtaacctgc cgcaacctgt ttgttaccag gctggaggaa
    28381 atcttcttcc gttaccggcg tgccaacagg cgtaacgcgg cggtagatgg agaaaatggt
    28441 accgacagag acgttaacat cgatgttgga cgagccatcc agggggtcca tcagcaccac
    28501 gtattttgcg tgttcacagc cttcaaagac gacaatctca tcttcttctt cagaggcaat
    28561 gcccgcaacg atatcgcgtg ctttcagtgc ggctttcagt ttttcattag cgaacaagtc
    28621 gagtttctgc tgaacctcgc cctgcacgtt ctcagcaccg ctggcaccca ggatatcaac
    28681 cagtcctgct ttgttgatat cgcgatggat aatcttggcg cccagtttta ttgccgacag
    28741 caaagcagtg agctcaccgg tagcatgaga aaactcgtgc tgcttttcga caataaattc
    28801 acctaacgtt ttcataaaac tttccctgca atgtttatgg agtaaagcga ccgcaacaat
    28861 cttaacaaat aatctcaatg ttgcgctcag gtgaatcgcg ccagcaaatt gcggattatc
    28921 ctgaaatgcg tttctcactt gcccgacata tgcgtaaaat gagcggcaga ttaaaaaagg
    28981 atagtgacgt atgcgcattc atattttagg aatttgtggc acatttatgg gcggtctggc
    29041 gatgctggcg cgccagttag gccatgaagt aacgggttcg gacgccaatg tgtatccgcc
    29101 gatgagcacc ttacttgaga agcaaggcat tgagctgatt cagggttacg atgccagcca
    29161 gctcgatccg cagccggatc tggtgattat tggcaacgcc atgacccgtg gaaatccgtg
    29221 tgtggaagcg gtactggaaa aaaacatccc ttatatgtca ggtccacagt ggctgcacga
    29281 ttttgtgctg cgcgaccgct gggtgctggc cgttgccggt acacacggca aaactaccac
    29341 cgcgggaatg gcgacctgga ttctggaaca gtgcggttac aaaccgggat ttgtgatcgg
    29401 cggtgtgccg gggaactttg aggtttcggc gcgtctgggc gaaagcgact tctttgttat
    29461 cgaagcggat gagtatgact gcgccttctt cgacaaacgc tctaaatttg tccattactg
    29521 cccgcgtacg ctgatcctca acaaccttga gttcgatcac gccgatatct ttgacgacct
    29581 gaaagcgatc cagaaacagt tccaccatct ggtgcgtatc gttccggggc agggccgtat
    29641 tatctggccg gaaaatgaca tcaacctgaa acagaccatg gcgatgggct gctggagcga
    29701 gcaggagctg gtgggtgagc agggtcactg gcaggcgaaa aagctgacca ctgacgcttc
    29761 tgaatgggaa gtcttgctgg atggcgaaaa agtgggcgaa gtgaaatggt cgctggtggg
    29821 cgaacataat atgcacaatg gcctgatggc gattgcggcg gctcgccatg ttggtgtagc
    29881 gcctgcagat gccgctaacg cgctgggttc gtttattaac gctcgtcgcc gtctggagtt
    29941 gcgtggtgaa gcgaatggcg tgacggtata tgacgatttt gcccatcacc cgacggcaat
    30001 tctggcaacg cttgcggcgc tgcgtggcaa agttggtggt acggcgcgca ttattgctgt
    30061 gctggagccg cgctcgaata ccatgaaaat ggggatctgc aaagacgatc tggcaccttc
    30121 attaggtcgt gccgatgaag tcttcctgct gcaaccggcg catattccgt ggcaggtggc
    30181 agaagtggca gaagcctgcg ttcagcctgc acactggagt ggcgatgtgg atacgctggc
    30241 agatatggtg gtgaaaaccg ctcagcctgg cgaccatatt ctggtgatga gcaacggcgg
    30301 ttttggtggg atccatcaga aactgctgga tggtctggcg aagaaggcgg aagctgcgca
    30361 gtaattcggc ctcagcctga tatagtgtgc gtaggccgga taaggcgttc acgccgcatc
    30421 cggcatttga gcatagtgcc tgatgcgacg cttgatgcgt cttatcaggc ctacaggtgc
    30481 accgcatccg gcaaaccata ctcacatcaa caacgaaaat tacccttcat tctccgccag
    30541 ttcgcgcaga tactggaaaa tctggcgtgc ggatttcggc ggcttattcc cttctttctc
    30601 tttcttcgcg ttgcggatca gagtacgcag ctgctgacga tcggcatcgg gccacagatt
    30661 taatacctca gcgatggcgt catcaccttg atcgatcaga cgatcgcgca ggttttccag
    30721 tttatggaac agcaccacct gctggttgtg acggtttttc agcttatcca gcgcctgacg
    30781 gatcggttct acatcgcgct ggcgcagcat cttaccgatg agctgcaact ggcggcggcg
    30841 accttccatc ttaatacgct gggcgagctc aatagccgcg cgcagatccg catctaacgg
    30901 gatcttatcc agcgcgtttt tccccagatc aacaatttcc gcgccaaggc gttttagctc
    30961 ctcggcatca cgtttaattt cacttttact gacccagata atttcatcgt cttcgtcttc
    31021 gatgtcatca ccgggaacgt cgtcgagcca gtcttcgggc tgcttagtca tctcaggctc
    31081 cttaaaaaaa gaggctaatg ttaccagtta agatgcgcac tgaaaaacgg ttctctgtta
    31141 gacttcagag aaactctcta cattatggca cttgcaatga aagtaatctc tcaagttgaa
    31201 gcgcagcgca agattctgga agaagcagtt tccactgcgc tggagttggc ctcaggcaaa
    31261 tcggacggtg cggaagttgc cgtcagcaag accaccggca ttagcgtaag cacgcgttat
    31321 ggtgaagtgg agaatgttga attcaatagc gatggcgcgc tggggatcac tgtttatcac
    31381 cagaaccgca aaggtagcgc atcatccacc gatttaagcc cgcaggccat tgcccgtact
    31441 gtacaggcgg cgctggatat tgcccgttat acctcgccag atccctgtgc cggcgtggca
    31501 gacaaagagc tgctggcctt tgacgcacca gatctcgact tgttccaccc tgcggaagtt
    31561 tccccggatg aagccattga actggcggcc cgcgcagaac aggcggcatt gcaggcggac
    31621 aaacgcatta ccaataccga aggtggcagc tttaacagcc actacggtgt caaagttttt
    31681 ggcaacagcc acggcatgtt gcagggttac tgctcaacgc gtcattcgct ctccagctgt
    31741 gtaattgccg aagaaaatgg cgatatggag cgtgattacg cctacaccat tggccgcgcg
    31801 atgagcgatc tgcaaacgcc agagtgggtc ggggccgact gtgctcgccg tactttatcg
    31861 cgcctgtcac cgcgtaaact ctccaccatg aaagcgccag tcatttttgc caatgaagtg
    31921 gcaaccgggc tttttggcca tctggtgggg gcgatagcgg gtggctcggt ttaccgtaaa
    31981 tctacctttc tgctggattc gctgggtaaa caaattctgc cggactggct gaccattgaa
    32041 gagcatccgc atctgctgaa agggctggcg tcgacgccat tcgacagcga aggtgtgcgc
    32101 accgagcgtc gcgatattat taaagatggc atcctgactc agtggctgct gaccagctac
    32161 tcggcgcgga aactggggct gaaaagcacc ggacatgcgg gcggtattca caactggcgg
    32221 attgccggac aagggctaag cttcgaacaa atgctcaaag agatgggcac cgggctggtg
    32281 gtgacggagc tgatgggcca gggcgtgagt gccattaccg gtgattattc ccgtggtgca
    32341 gcgggcttct gggtagagaa tggcgaaatt cagtatcccg ttagtgaaat caccatcgca
    32401 ggtaatttaa aagatatgtg gcgcaatatt gtcaccgtcg gtaacgatat tgaaacacgc
    32461 agtaatatac agtgtggttc tgtgctgttg ccggagatga aaatcgccgg gcagtaaaaa
    32521 actggcgcga cctgccgcgc tataaactaa aaaattacac aaaatcattc gcactgcatc
    32581 gagacggaaa gtgaatgagg gccggggagc gtactcgcag tacgtgaccc gggcgaatga
    32641 gcgtagccgt cgatgaggca gtgtgaagga tgaagtgtaa ataaaaaagg aagtgagcaa
    32701 tgcgtaaaag cctgttagct attcttgcag tctcctcgtt ggtattcagt tctgcgtcgt
    32761 ttgccgctga tcttgaagac aatatggaaa ccctcaacga caatttaaaa gtgatcgaaa
    32821 aagcggataa cgcggcgcaa gtcaaagacg cgttaacgaa gatgcgcgcc gcagcgctgg
    32881 atgcgcaaaa agcaacgccg ccgaagctcg aagataaatc accggacagc ccggaaatga
    32941 aagatttccg ccacggtttc gacattctgg tcggtcagat tgacgacgcg ctgaagctgg
    33001 caaatgaagg taaagtaaaa gaagcgcagg ctgctgcaga gcaactgaaa acgacccgca
    33061 acgcctatca ccagaagtat cgttaattcc tcatttccct gttgcctgca ctcaggtaac
    33121 agggaactta tcatcgcaaa tgatgcacca cctgattgct gctgccgcgc cagatcaggg
    33181 aagggtcttt taaatcctgc acaaatttgc cgtcgaccag tacgttaatc aaatcaacaa
    33241 cctgcatttg cgcagcgttg agttcgtcga gtttatagcc tgtccacacc cagatgtctt
    33301 tacccggaca ctcggcgcgg atgcgtttta ccagtttcag aatatccggc acgttttgcg
    33361 gatgcagcgg atcgccgccg gagagggaaa taccctggcg tttgatacga gtgtcattca
    33421 gatcgttaat aatctggtct tccattgctt tagtaaatgt ctgaccggaa tttacccgcc
    33481 atgtgctttt gttatagcaa ccggggcatt catgaacaca cccggagaca aacagggtgc
    33541 agcgagtgcc ggggccgttg acgatgtcga caggatagta ctgatgataa ttcattgttt
    33601 cgcctggctt atcagtccgc gtataggatg catccggtaa tcaacgcctg atgcgacgct
    33661 gtcgcgtctt atcagaccta caatcgttgt tgccatgtag gccggataag gcgtttacgt
    33721 cgcatccggc atttgcttaa cgtagagtga agattaacct atctgcccat tccccaaatg
    33781 tttaacgcgg cgcttaactt cttcctgctt accggcgtta aatggacgtg catccgggct
    33841 acctaaatat ccgcacacgc ggcgagtcac cgacacacgg gaggcgtcat ggttaccaca
    33901 tttcgggcaa gtgaagcctt tgctggtgca ctcgaactca ccggtaaagc cacactcgta
    33961 gcactcatca atcggtgtat tggtgccgta atacggtaca tgctgatagc tgtaatccca
    34021 gacatcttcc agcgccttca ggttgtgctg aatgtttgga tactcgccgt agcaaatgaa
    34081 accaccgttc gccagcggcg ggtaaggcgc ttcaaagtcg atcttgtcgt acgggttcac
    34141 cttcttctcc acatcgaggt ggaaactgtt ggtgtagtaa cctttatcgg tcacgcccgg
    34201 caccacgcca aactcagcag tatcgagacg gcagaagcgg tcgcacaggt tttcactcgg
    34261 cgtgctgtag agactgaaac cataacccgt ttcttctttc cactgatcca ctgcctggcg
    34321 cagacgttca acaatggcga tacctttcgc gcgaagctgc tcgttgtcgt aaacatgctc
    34381 gccgccgaac agcgcgttaa tggtttcgtg gatgccgatg taacccagcg aaatagacgc
    34441 acgaccgttt ttgaagattt cagaaacatc atcgtcagca ttcagacgta cgccacaagc
    34501 accttccata tagaggatcg gggccacgcg cgctttcacg ccttcgagac gagcgatacg
    34561 ggtcatcagc gccttacgtg ccagcaccag acgttcatcc agcagcttcc agaaggtggc
    34621 ttcatcgcct tttgcttcca gagcaatacg cggcaggttc aggctgatca cgccgaggtt
    34681 gttacgacca tcgtggatct gctcgccgtt ttcattttcc cacacgccga ggaagctgcg
    34741 gcagcccatc ggggttttaa acgaaccggt gactttcact acctgatcgt agttcaggat
    34801 atccggatac atgcgcttgc ttgcgcactc cagcgccagc tgtttgatgt cgtagttcgg
    34861 atcgcctttt ttatggttca gaccatcgcg aatcgcaaac accagtttcg ggaacaccgc
    34921 agttttacgg tttttaccga ggcctgcgat acggttacgc aggatcgatt cctgaatcag
    34981 gcgcgattcc cagctggtgc ccagaccaaa accaaaggtt acaaacggcg tctgaccgtt
    35041 ggcggtgtgc agggtgttta cttcgtactc cagcgactgg aaggcatcgt agcactcttt
    35101 gatggtacga gagtttgcgt agccttcggc gtccgggatg ttccactctt ctgcggtctt
    35161 gcgatgtttg ttgtagctgg cggtaacaaa cggcgccagc acttcatcga tacggttaat
    35221 ggtggtgccg ccataaatat ggctggcgac ctgagcaata atctgcgcag ttaccgcggt
    35281 tgccgtagag atcgacttcg gcggttcaat ctcggcgttc cccatcttaa agccctgggt
    35341 cagcatgcct ttcaggtcga tcaacatgca gttgaacatc gggaagaacg gcgagtaatc
    35401 gagatcgtga tagtgaatat cgccacgctc atgtgcctgc accacgtcac gcggcagcag
    35461 gtgctgacgt gcatagtgtt tagccacgat cccggccagc aggtcgcgct gggttggaat
    35521 caccttgctg tctttgttgg cgttttcgtt gagtaacgag gcgttggtct gctcgaccag
    35581 accacggatc tcctggttca ggcgaccgcg tttttcacgt tcaatgtcgc gatcgtgacg
    35641 gtactcgatg taagcacgag ccagttgttt gtatggaccc gacatcagct ggttttcaac
    35701 tgcggtctgg atctcgttga tatccacctg gttgcggccc tgcatctgct cgctgacaac
    35761 cgcggcaaca gtggcgcaat aatcggcatc atcgacttcc gctgctttag ctgcacgcag
    35821 aattgcttct ttgatgcgct ctgatttaaa cggcactttg cagccgtctc gtttcatcac
    35881 atgcggtgtc atgatcgctc catattttta agaacaggtt atccacagaa attgggaaag
    35941 gcgttttccg gttttttcgt ttcgttttcc ggtgctttcc gcaatcgcca tccgcttatc
    36001 cacaacgccg cacctttttc gtggggagct gtagtagcaa ttatagtcga ttaatacaac
    36061 atattgggtt ggggcgcatt ttaaagtcta tatatagtgc tttgcatcaa ggatgtttta
    36121 actttttttg atgtagctca aagtaaaaag cagagcgtac ggatgacggg cgctacagcg
    36181 ttatgtaaat tttttaatga atttgctggt tgaaaaatca acaaaaagaa catgctgaca
    36241 gacaaaaccc cggaatgacc ggcattaccg gggcttaggg aagatttact tctgtaacca
    36301 ccagacagcc tcaaaaggcc gtaaattcat ggcacagggt tgtggtgagg cttcttcgta
    36361 gttatgcatc acaagctgcc agttgccgcg catttgccct ggctgccagg gttggatctc
    36421 acggctaagg ttggcaatga ccagcaaggt ttgccccttc cattcacggc gatagcacca
    36481 caatacaggg ctgtttggca gcagatcctg gtaattgccc catgtcagga tggcttcttg
    36541 cttacgcagt gcgattaact tttggtaggt gtaaaacacc gaggagtcat cggccagcgc
    36601 ggcttctacg ttgatttgtt gatagttatc gcccaggcca atccacggtt cgccagccgt
    36661 aaacccggca ttatcgccgt tgctccattg catgggcgtg cggctgttgt cacgggattt
    36721 actggcgagg attgccaata actcgtcggc atcacgacca tcgttgcgca gctcggcaaa
    36781 catattgagg ctctccacgt cgcgatagtc agtaatgcgc gtgaaatgcg ggttggtcat
    36841 gccaatctct tcgccttggt agatatacgg cgttccctgc atgccatgca gcaccatcgc
    36901 cagcattttt gccgcaggca cgcggtattc accttcatca ccaaagcgag aaacaatgcg
    36961 cggctgatcg tggttacacc agaacaaggc attccatgcc acgttgtgca ttccttgttg
    37021 ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt ttagccagcg tccatttttc
    37081 accaccggga taatcgacct tcaggtgatg aaaattaaag gtcatcgaca attcactgcc
    37141 tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg gaggacattt cacctacggt
    37201 cattaacccg cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg
    37261 tggcccgtcg gtgtagaagc gacgcccgtc gccatccagg tcttcaggga aacgcgggtc
    37321 tttggagatc agattcacca catccaggcg caacccgtcg accccacgat cggcccagaa
    37381 ttcacagact tttttcagct ctgcgcgtac cgctggattc tcccagttga gatccgcctg
    37441 ttctggtgca aagagatgca aatagtactg ttcgctttcc gcatgccagc gccacgcact
    37501 accgccaaat tttgaacgcc agttgttcgg tggcgtttct ggttcgccat cgcgccagat
    37561 ataaaactgg cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg catgttgggt
    37621 agaggtatgg ttaaacacca tatcgagaat gatacgaatc ccgcgcgatt ttgcctgcgt
    37681 caccagttcg tcaaaatcgt ccagcgtgcc gtagatggga tcaatcgccg tatagttcgc
    37741 tacgtcgtaa ccgttatcga cctggggaga gatataaaag ggggttagcc agatggcatc
    37801 aacgcccagt ttatgcagat agtccaggcg ttggataacg ccacgtaaat cgccggtgcc
    37861 gctacccgtg gtgtcctgaa aactctttgg ataaatctgg tagataacgc cgttttgcca
    37921 ccagtgggga agattagtca ttacgttatt cctgcaaatg cgagggggcg caattgcgcc
    37981 ccgaagaaaa ttaaacaata tccagcgtgc ccaggcggta tttccgctga tagataaacg
    38041 aggtgagtac aatcgggatg atgatggcga taaccattgc cagcgcaaat acctgccagt
    38101 agctcggttg aatcgagaga attcccggca ggccgcctac gccgatacca ttcgccataa
    38161 cgccgttcag gccgcatagt aatcctgcca gaccagaacc aatcatcgcg cacagcatcg
    38221 ggaagcgata tttcaggttg atgccgtaca ttgcaggctc agtgacccca agccaggcgg
    38281 agatagcggc aggcacggag atctcgcgtt cattgtgctt gcggctggaa atgataatgc
    38341 ctatcacggc ggagccctga gcgatattcg acagcgcaat cagcggccac actggcgtgc
    38401 cacccatgct ttgaatcatc tgcaaatcaa tagcaagggt ggtctggtgt acaccggtga
    38461 tcaccagcgg ggcgtacagg aagccaaaca atgctgcgcc aatcggagca aagctgccgg
    38521 tcatcaggtg acgtaccgca aaggcaacgc catcgccaat catgcgacca aacggaccaa
    38581 tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca gacgggcacc accaccagat
    38641 agaggtaatc cggcacgatg cgtttaaggc gagtttcaat aacgcccagc gccagcccgg
    38701 ctaacagtgc cgggatcacc tgcgcctgat agcctacttt ggcgatgctg aacatgccaa
    38761 agtcccacac ttccggcagc tgctgcccga gcagataagc gttcatcagc tggggagaaa
    38821 ccagtgtcac gccaagcacg ataccaagga tcggcgtgcc gcccattttt ttcaccgctg
    38881 accagcaaat accgaccggc aggtagaaga agatcgcttc accgatcaac cacagaaaat
    38941 cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt ctgaccgttg ctcatgggca
    39001 aatcgccgat cacattgcga aaaccgagga tcaaaccgcc gctaatcaac gcgggcagca
    39061 gcgggaagaa gatctccgcg aaatgagaga tcaactgctc atgccatttc atattctgcc
    39121 gggcggcttt ttttacctgc tctttatcaa cctgcgcctg tccggttgac gcaatcagtg
    39181 cttgatagta atcacccaca ttggtgccaa tcaccacctg aaattgcccg gcattggtga
    39241 aacagccttt caccatgggg agttgctcaa tttctttcgg tctggcattg gccggttggt
    39301 tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc
    39361 cgaccagttc aatcaaccga tcgatatccg tttggtttat tttgctcatc ataaagcccc
    39421 atggcagatg acatttttgg ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg
    39481 gaacgttccc gaagtgcagc gaagatcaca atttatcgtt caggaaacga tcaggacagg
    39541 gtggcgggga tgatgatttg ttgcggttcg ctgcgcccgg ttacctgtgc gatcaattgg
    39601 caggccgcct ggcgtccagc ttcggcgtaa ccaggatcta cggtgacgat ctccggatga
    39661 aggaatttca ttaatggcgt attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc
    39721 tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag taaggcggta
    39781 gtttcaggcg taatcacttt tgctacgttc tcatagcctt gcttcatagc aaggcctggc
    39841 agagcggcaa cgggattcag tttatgcgct ttgcagaacg ccaggtaggc ttcgtgacgt
    39901 cgcttcccgg tcgtcacgtc actatgcggc acgccgagat aactgatatt acgatgcccc
    39961 tggtcataca gccgttgcat caggatcttg attgcccctt cgtcgtcata acagaccgaa
    40021 gcaaaacctt ttgcgtcacg cgccagcaga accagcgatg actgccagtg ggctaacatt
    40081 tcttctgtta tgccagtaaa accgaacagc actacgccgt cgatattacg ccgtttcagc
    40141 acccccaaat gttcggcaac taattgcggg gaaaactgac tttccatcat gattgggtcg
    40201 taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc tgacaacgaa
    40261 tccaggcggg taacaatgat ggcgaccact ttatcgcttt gcccacgcat agcgcgcgca
    40321 gagcgggaag gggaaaatcc atgctgattc atcactgctt caacacgctc gcgggtgcgc
    40381 tggctcacgc cgctttcgtt attcagcacc cgggaaactg tagatttccc cacgccgctt
    40441 aagcgtgcga tgtctttgat ggtcagccga ttttgcatcc tgttgtcctg taacgtgttg
    40501 tttaattatt tgagcctaac tttacccgtg cattcagcaa tgggtaaagt ctggtttatc
    40561 gttggtttag ttgccagcag gtattatatc gccatagatg ctacgaatat tattggattc
    40621 tccttattat ttgcggcgct tttttcactt accggaggtt atatggaacc tgatcccacg
    40681 cctctccctc gacggagatt aaaacttttc cggtaagccc gtcttttcac ggcgttaccg
    40741 gatgcgtaag gccgtgacgt tttaacgtcc ctgctcagct ttattacctt caggtaaggc
    40801 ttcgccacgc ctgaagacat ttctgtactg tttcagacag tgcggaggga ctccttatgt
    40861 ttaaagaaat ttttacccgg ctcattcgcc atttaccttc ccgtctggtt catcgcgatc
    40921 cattgcctgg cgcgcagcag acagtgaata cggcggtccc gccgtcctta agtgcgcatt
    40981 gcctgaaaat ggcggtgatg cccgaagaag aattatggaa aacgttcgac acccatccgg
    41041 aagggttaaa tcaggcggaa gtggaatctg cccgcgaaca acatggtgaa aataaattac
    41101 ccgcacaaca accgtcgccg tggtgggtac atttatgggt ctgctatcgc aaccccttta
    41161 atattttact caccattctc ggcgctattt cttacgccac ggaagattta tttgccgcgg
    41221 gcgttatcgc gctaatggtc gctatttcta cgttgctgaa ctttattcag gaagcacgtt
    41281 ccactaaagc ggcagatgcc ctgaaagcga tggtcagcaa tacagcgacg gttctgcgcg
    41341 taattaacga caaaggcgaa aatggctggc tggagatccc gatcgaccag ctggtgcccg
    41401 gcgatataat aaaactggcg gcgggggata tgatcccggc agatttacgt atcttgcagg
    41461 cgcgggatct gttcgtcgct caggcgtcgt taaccggtga gtctctgccc gtagaaaaag
    41521 ccgctaccac tcgccagccg gagcacagca atccgctgga gtgtgacacc ctgtgtttta
    41581 tgggcaccac cgtggtgagc ggcacggcac aagcaatggt gattgctaca ggtgccaata
    41641 cctggtttgg tcaactggcg gggcgtgtta gtgagcagga aagcgagccg aatgcctttc
    41701 agcaagggat cagccgcgtc agtatgctgc tgattcgctt tatgctggtg atggctccgg
    41761 tggtgctgtt aatcaatggt tacaccaaag gcgactggtg ggaagcggca ctgtttgcgc
    41821 tttcggtcgc ggtaggctta acgccggaaa tgttgccgat gattgttacc tcgacgctgg
    41881 cgcgcggcgc agtaaagctg tcgaaacaga aagtgatcgt caaacatctg gatgctattc
    41941 agaactttgg cgcaatggat attctgtgca ctgataaaac tggcaccctg acacaggaca
    42001 aaattgtgct ggagaatcat accgatatct ccggtaaaac cagcgaacgc gtgctgcata
    42061 gcgcgtggtt gaacagtcat tatcagaccg gacttaaaaa cctgctcgat acagcggtgc
    42121 tcgaaggtac ggatgaagag tcagcgcgct cgctggccag tcgttggcaa aaaattgatg
    42181 agattccgtt tgatttcgag cgtcgccgga tgtcggtggt agtggcagaa aataccgagc
    42241 accatcagct ggtttgcaaa ggtgcattgc aggaaatcct caatgtgtgt tcgcaggtgc
    42301 gtcacaatgg cgagattgtg ccgctcgatg acatcatgct gcgtaagatt aagcgggtta
    42361 ctgatacgct taatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa tacctgccag
    42421 cgcgtgaagg agattaccag cgggcggatg aatccgacct gatcctcgaa ggatatattg
    42481 ctttccttga tccgccaaaa gagacaaccg ctccggcact gaaggcatta aaagcgagtg
    42541 gaatcaccgt aaaaatcctt actggcgaca gtgagttagt tgcagcgaaa gtgtgccatg
    42601 aagtggggct ggacgcggga gaggtggtca ttggtagtga tattgaaacg ctatctgacg
    42661 acgaactggc aaatctcgca cagcgcacca cgctgtttgc ccgcctgacg ccgatgcata
    42721 aagaacgcat tgtgacctta ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg
    42781 gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg gacggcgcgg
    42841 tagatattgc ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg atggtgctgg
    42901 aagagggggt tattgaagga cgtcgcactt tcgccaacat gctgaaatac atcaaaatga
    42961 cggcgagctc caacttcggt aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct
    43021 tcctgccgat gttgccgtta cacttgctta ttcagaacct gctgtatgat gtgtcacagg
    43081 tggcgatccc gtttgataac gtcgacgacg agcaaattca aaagccgcag cgttggaatc
    43141 cggcggatct ggggcgcttt atgatcttct tcggaccgat cagctcgatc ttcgatattc
    43201 tgacgttttg cctgatgtgg tgggtattcc atgccaacac gccggaaacg caaacgctgt
    43261 tccagtcggg atggtttgtg gtgggcttac tgtcgcaaac gctgattgtg catatgatcc
    43321 gcacccgccg tgtgccgttt attcagagct gtgcatcgtg gccgttaatg atcatgaccg
    43381 tgatcgtgat gattgtcggg atcgcattgc cgttttcacc gctggccagt tatctgcagc
    43441 tgcaggcgct gccgttaagc tatttcccgt ggctggttgc gattctggca gggtatatga
    43501 cattaactca gttggtgaaa ggattttata gccgtcgtta cggctggcag taaagaataa
    43561 actgggcacg atagccccga ttcagattgc agacaaagta agctttgttc atgccggatg
    43621 cggcgtaaat gccttatccg gccaacaaat tcaataaatt gcaggaatca cgtaggcctg
    43681 ataagcgtag cgcatcaggc gattttgcgt ttgtcatcag acttaatccg ggcatgatag
    43741 cccggatttc catcaagatt agcgacgaac agcgatcgct tcgatctcaa tcttcacgtc
    43801 tttcggcaga cgggcaactt caacgcaaga acgtgccggg aaggtggcat tgtgttcggt
    43861 gaagaaggct tcgtaggtag cattaacggt tgcgaagtcg ttcagatctt tcacaaacac
    43921 ggtggtttta acgatgtcgc ccactttcag gcctgcggct tcgacgatcg cttttacgtt
    43981 atccagcgac tgacgtgcct gtgcagcgac gtctgccggt acttcgcccg ttttcggatt
    44041 taccgggatc tggccggagg tgatgatcat attgcccaga tcaacgccct gtacgtaagg
    44101 accgatagct gccggtgcat tttccgtcgc gatagttttg ctcatgattt ctcctttatt
    44161 acagcggtaa aaagtctggc tcattatagg gagccagact tttattacca accgcaatta
    44221 attggccagc accacattat gggaaaactc tttttcacag tatttgcatt tgagcgcgat
    44281 gtcattggcg cgttttcgca cggcaaagct ggaagaaacc ggttcggcat ggctgataca
    44341 gttgctgttc gggcagacca gcacattgtc gatgcgctct ggcagactgg ggcgcgattt
    44401 acccaccact tcatagttgt cgatacggtt aaccgtggct tgcggcgcat acaatgccag
    44461 ttgatctacc tgatcttcac tcaaaaaggt attttcgatt ttgatcagat ctttgcggcc
    44521 catctcgcca gaaggcaagt tcagaccgat ggtgatgcgc tgatccgttt cggtcagctt
    44581 gaacagactc aacagcttaa aaccgatctg ggcggggata tggtcaatta ccgtgccgcg
    44641 tttaatagct tcaacctgca atttattatc gtgtgtcatc tctatttccc cttacagtac
    44701 cagatcgcga ttcagaacca gtgccagtaa cgcctggcga gcgaaaatcc cgttgcctgc
    44761 ctgctggaag taccaggcgt gtggcgtttt atcaacatcc gtcgcaatct catcaacacg
    44821 cggcagcgga tgcagcactt tcatattggc tttggcgttg tggagatcgc tggcgcgaag
    44881 aacaaactgc gctttcacgt tggcgtactc ggacgggtcc agacgctctt tttgcacgcg
    44941 ggtcatgtac aggatgtcta cttccgccat cacttcttca atagagctgt gcagactcca
    45001 tgcgatccct ttttcatcga gcatatccag aatgtattgc ggcattgcca gcgcgtccgg
    45061 cgcgatgaag taaaaacggt tgccgtcgaa cttcgctaac gcctgagtca gggagtgaac
    45121 ggtgcggcca tatttcaggt caccaaccat tgcgacgtgg agattgtcca gacgcccctg
    45181 ggtttcctga atagtgaata agtccagcaa ggtttgcgtc ggatgttggt tggagccatc
    45241 accggcattc agtaccggta cattgccgga aaactcggtg gccaggcgcg ccgcaccttc
    45301 ctgcggatga cgcatcacta tcgcatcgac gtaagtgctg ataaccgaaa tggtatcggc
    45361 cagcgtttcg ccctttttac ccagtgatgt attggcgctg tcggagaagc ccaccacgct
    45421 ggcacccagg cggtgcatag atgtttcgaa agagaggcgg gtacgggtag aggcttcgaa
    45481 gaaacagctg gcaatgactt tgtgcttcaa cagctctggt tgcgggtttg ctttcagttt
    45541 cgccgctgtc gccagcacca gattaaggtc atcgcgacta aggtcgttta tggaaatgat
    45601 atgtttctga tatagcggat tagccatctt ttatctcctg acgcctgggc aaaaaaaagc
    45661 ccctcgattg aggggctggg aatgggtgat caacgggaag aaaaacggca ggccagcgtc
    45721 ttttttcaga cgcggtaaga caaaatgtcg aacacactga accatacatc ctcccggcaa
    45781 attgtccggc attatactca tcgtcagaag cggtgcaagc atttgatgca atattttgtc
    45841 agcgcaaacg gtttatttga attaaaagtc aaggtatatg catttttatt tgatgtgatt
    45901 ctgcagggga actgtccttc ggtatcaata attgcagaca ttccctgctt tttccttttt
    45961 cactttcacg caatcagata tgcattttat tccattcatc cgacttatag ggcggagtgt
    46021 gaaagcgaac ggctaacact attgcttact gctcagggat gcgcgctatc actttaattt
    46081 caaaatcaaa gcctgccagc catgtaacac ccaccgccgt ccagtttgga taaggtgggg
    46141 cgctaaatat ttcatttttc accgtcatga tgtcttcaaa ttggttttct ggatcggtat
    46201 ggaagctcgt aacatcaatg atatcgtcaa aagtgcatcc cgcagctgcc agggtcgcat
    46261 gcaaattatc aaatgccagt ctgacttgtt gctgaaaatc gggttctggt gttccgtcct
    46321 ctcgacttcc tacttgcccg gaaacaaaca gcaaatcgcc ggaacgaata gccgcagaat
    46381 aacgatgctc agcatatagt gaatgtcggc cagcagggaa aacagcggtt ctttctacca
    46441 tttggttatc ctcaagattt acgacatgaa cagaagattt ctctttaccg ggagccgctt
    46501 ttagcggacg acgtgagtaa acaaaaccca gacatcatgg ataatggctg ggcttaattg
    46561 agcgtagtcg gttatgcgcc aaacgcgcca tcaatggtat gcatcgcgcc ggtaacaaaa
    46621 ctggcttctg gccctgctaa ccatgcgacc ataccagcga cctcttccgg ttgcccatgt
    46681 cttttgatag ccatcaaact atgcaacata tcgcgcattg gcccgttggc gggattagcg
    46741 tcggtatcaa ttggccctgg ctggacgacg ttaatggtga tcccacgcgg tccaaaatca
    46801 cgggccagcc cgcgcgccat gccttgcagg gcagatttgc tggcggcata agcagccatg
    46861 cctgcaacag gcatacgatc gccattcacg gagccgatga ttaagatgcg cccgccttcg
    46921 ggcatctgcc gggcggcttc aacagaggca tgataaggag catgaatatt gattttgaaa
    46981 aggcgatcaa tatcgtcggc atttaattcc agggcctcgc caaagacgcc aatacctgca
    47041 tttaccacca ggatatccaa tgcgccgctc ttacgaacga catcaatgac agcgtctctg
    47101 tcagcactat ctgtgaatac tgctgtcgct ccagtctctt gtgccaggcg tttagcggca
    47161 tctttcgacc ccgcataggt gaatcgtaca ttggccccat cggtgacgaa acgacgtacg
    47221 atagcggcac cgataccacg actgccaccg aggatgagaa ctgtcttacc tgtaaaagcg
    47281 cccataagga ctccttgatt tattatgtaa catgcattac aaaactgttt taactttctg
    47341 tcaacaagtt ttgtaatggt cactaaaaaa caatctcgcg ttccaggtcg tcccagacgt
    47401 ttcgctcctg agcaggcagt ctctgcggca aaagtgcttt ttcaccaaaa aggtttcgat
    47461 gctgtcagtg ttgctgaagt tactgattat cttggtatta accccccgag tctctacgcg
    47521 gcttttggca gtaaagctgg gttatttagc cgtgtactca atgaatacgt cggtacggaa
    47581 gctattccgc ttgccgatat tcttcgtgat gatcgtccag taggcgagtg cctggttgag
    47641 gtattaaaag aagcggcgcg cagatatagc caaaacggcg gctgcgctgg ctgtatggtt
    47701 cttgaaggta ttcatagtca tgatccacta gcgcgtgata ttgccgttca atattatcac
    47761 gccgcagaaa cgaccattta tgactatatc gccaggcggc atccacaaag cgcacaatgt
    47821 gtgactgatt ttatgagtac cgtgatgtca gggctttctg cgaaggcacg ggaggggcac
    47881 tcaatcgaac aactctgtgc aacagctgca ctggcggggg aagcgataaa aactcttctc
    47941 aaggagtgat gctggccttg atccgaaagg cgggaacgcg cctgccgata agttgtgata
    48001 agacaataat tcacgcgtta aggctggcgg aattgatcat cttttcgtat aacgatagaa
    48061 atgaaacatt gttttaatta aggagtggaa aagatgatca tcggaaatat tcataatctt
    48121 cagccgtggc taccccagga gttacgccag gcgattgagc atatcaaagc gcacgttacg
    48181 gcagaaacgc caaagggtaa gcacgatatc gaaggcaatc gtctgtttta tcttatctcg
    48241 gaagatatga ccgagccgta cgaagctcgc cgtgcggagt atcatgcccg ctatctcgac
    48301 attcagattg tgttaaaagg tcaggaaggc atgaccttca gcacgcaacc tgcaggcacg
    48361 ccggataccg actggttagc tgataaagac atcgcatttt tgccggaagg cgttgatgag
    48421 aaaacagtta tccttaatga aggtgatttt gttgtgtttt atccggggga agtgcataaa
    48481 ccgctgtgcg cagtgggcgc gccagcccag gttcgcaaag cagtagtgaa gatgctgatg
    48541 gcgtgatgac ttttcgccgt aaataactcc aggtttacgg cgagtttgtg aaaagagcgt
    48601 tttttgatat ttttttgtga gtagaatttg taatacttag acgttcttat taactcaagg
    48661 agtccgtcat gagcaaaata tcaggttgga atttttctca aaacattaca tcagccgaca
    48721 attgtaaaca aaaaaatgaa gacttagata cctggtatgt gggaatgaat gattttgccc
    48781 gaattgccgg agggcagaat agcagaagca atattctttc tcccagagca tttttggagt
    48841 ttttggctaa gatatttacc ctgggttatg tggattttag caaacgctcc aacgaagcgg
    48901 gtagaaatat gatggctcat attaagtcct catcttatat caaaaataat gatggtagtg
    48961 agataatgaa gtttgttatg aataatcctg aaggggagcg agcggattta tctaaggtgg
    49021 aaatagaaat tacacttgcc tcagctttta ataatgggat tcgtgaagga catacagtca
    49081 ttatatttac acaacctgat ggttcgacta atcgttatga agggaagtcc tttgaaagaa
    49141 aagatgagag ttcattacac ctgattacta acaagattct ggcgtgttac caacgcgaag
    49201 ctaacaagga aatagctcgt ctattaaata ttccgcagga gttaaataat tctcaggact
    49261 tgaataactc gcaggtgagt tgtaaagatt cagttgattc tacgattagg gatttattag
    49321 aaaaaccatt gaataatgca ttattagcaa taaggaaaga acatctgcta ttaatgcctt
    49381 atgtatgtaa tgaatcgatt tcatacttgc ttggtgaaaa agggatactg aaagaaatag
    49441 atgaccttaa cgcagtaaat aattatcttc ttaataataa gaaagctact gacaatgaaa
    49501 ttaatgacat caaagttaac ctgtctcata ttcttattga t
//
LOCUS       DAZGTR010000019        49133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGTR010000019 DAZGTR010000000
VERSION     DAZGTR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.019"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHLCY_001281"
     CDS             complement(96..827)
                     /gene="rlmB"
                     /locus_tag="ACHLCY_001281"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP0368581.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELICIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1007..3367)
                     /gene="rnr"
                     /locus_tag="ACHLCY_001282"
     CDS             complement(1007..3367)
                     /gene="rnr"
                     /locus_tag="ACHLCY_001282"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP0368582.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKKVAE"
     gene            complement(3406..3831)
                     /gene="nsrR"
                     /locus_tag="ACHLCY_001283"
     CDS             complement(3406..3831)
                     /gene="nsrR"
                     /locus_tag="ACHLCY_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP0368583.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4036..5334)
                     /gene="purA"
                     /locus_tag="ACHLCY_001284"
     CDS             complement(4036..5334)
                     /gene="purA"
                     /locus_tag="ACHLCY_001284"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP0368584.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5438..5635)
                     /gene="yjeT"
                     /locus_tag="ACHLCY_001285"
     CDS             complement(5438..5635)
                     /gene="yjeT"
                     /locus_tag="ACHLCY_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP0368585.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5717..6721)
                     /gene="hflC"
                     /locus_tag="ACHLCY_001286"
     CDS             complement(5717..6721)
                     /gene="hflC"
                     /locus_tag="ACHLCY_001286"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP0368586.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6724..7983)
                     /gene="hflK"
                     /locus_tag="ACHLCY_001287"
     CDS             complement(6724..7983)
                     /gene="hflK"
                     /locus_tag="ACHLCY_001287"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP0368587.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8069..9349)
                     /gene="hflX"
                     /locus_tag="ACHLCY_001288"
     CDS             complement(8069..9349)
                     /gene="hflX"
                     /locus_tag="ACHLCY_001288"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP0368588.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGALVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9426..9734)
                     /gene="hfq"
                     /locus_tag="ACHLCY_001289"
     CDS             complement(9426..9734)
                     /gene="hfq"
                     /locus_tag="ACHLCY_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP0368589.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9820..10770)
                     /gene="miaA"
                     /locus_tag="ACHLCY_001290"
     CDS             complement(9820..10770)
                     /gene="miaA"
                     /locus_tag="ACHLCY_001290"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP0368590.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAVEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10763..12610)
                     /gene="mutL"
                     /locus_tag="ACHLCY_001291"
     CDS             complement(10763..12610)
                     /gene="mutL"
                     /locus_tag="ACHLCY_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP0368591.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRPIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12620..13957)
                     /gene="amiB"
                     /locus_tag="ACHLCY_001292"
     CDS             complement(12620..13957)
                     /gene="amiB"
                     /locus_tag="ACHLCY_001292"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP0368592.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13976..14437)
                     /gene="tsaE"
                     /locus_tag="ACHLCY_001293"
     CDS             complement(13976..14437)
                     /gene="tsaE"
                     /locus_tag="ACHLCY_001293"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP0368593.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14409..15956)
                     /gene="nnr"
                     /locus_tag="ACHLCY_001294"
     CDS             complement(14409..15956)
                     /gene="nnr"
                     /locus_tag="ACHLCY_001294"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP0368594.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15955..17094
                     /gene="queG"
                     /locus_tag="ACHLCY_001295"
     CDS             15955..17094
                     /gene="queG"
                     /locus_tag="ACHLCY_001295"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP0368595.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFLQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAAKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAMAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17077..17130)
                     /locus_tag="ACHLCY_001296"
     CDS             complement(17077..17130)
                     /locus_tag="ACHLCY_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368596.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17364..17439)
                     /locus_tag="ACHLCY_001297"
     tRNA            complement(17364..17439)
                     /locus_tag="ACHLCY_001297"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17404..17406),aa:Gly,seq:gcc)
     gene            complement(17475..17550)
                     /locus_tag="ACHLCY_001298"
     tRNA            complement(17475..17550)
                     /locus_tag="ACHLCY_001298"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17515..17517),aa:Gly,seq:gcc)
     gene            complement(17708..17783)
                     /locus_tag="ACHLCY_001299"
     tRNA            complement(17708..17783)
                     /locus_tag="ACHLCY_001299"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17748..17750),aa:Gly,seq:gcc)
     gene            complement(17994..18539)
                     /gene="orn"
                     /locus_tag="ACHLCY_001300"
     CDS             complement(17994..18539)
                     /gene="orn"
                     /locus_tag="ACHLCY_001300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0368597.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18634..19686
                     /gene="rsgA"
                     /locus_tag="ACHLCY_001301"
     CDS             18634..19686
                     /gene="rsgA"
                     /locus_tag="ACHLCY_001301"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0368598.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19783..20751
                     /gene="asd"
                     /locus_tag="ACHLCY_001302"
     CDS             19783..20751
                     /gene="asd"
                     /locus_tag="ACHLCY_001302"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0368599.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20773..24096
                     /gene="mscM"
                     /locus_tag="ACHLCY_001303"
     CDS             20773..24096
                     /gene="mscM"
                     /locus_tag="ACHLCY_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0368600.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24125..24439)
                     /gene="yjeO"
                     /locus_tag="ACHLCY_001304"
     CDS             complement(24125..24439)
                     /gene="yjeO"
                     /locus_tag="ACHLCY_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFP0368601.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24436..24750)
                     /gene="yjeN"
                     /locus_tag="ACHLCY_001305"
     CDS             complement(24436..24750)
                     /gene="yjeN"
                     /locus_tag="ACHLCY_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFP0368602.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24802..26304)
                     /gene="yjeM"
                     /locus_tag="ACHLCY_001306"
     CDS             complement(24802..26304)
                     /gene="yjeM"
                     /locus_tag="ACHLCY_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0368603.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26523..27500)
                     /gene="epmA"
                     /locus_tag="ACHLCY_001307"
     CDS             complement(26523..27500)
                     /gene="epmA"
                     /locus_tag="ACHLCY_001307"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0368604.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27825..29633
                     /gene="frdA"
                     /locus_tag="ACHLCY_001308"
     CDS             27825..29633
                     /gene="frdA"
                     /locus_tag="ACHLCY_001308"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0368605.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29626..30360
                     /gene="frdB"
                     /locus_tag="ACHLCY_001309"
     CDS             29626..30360
                     /gene="frdB"
                     /locus_tag="ACHLCY_001309"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0368606.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30371..30766
                     /gene="frdC"
                     /locus_tag="ACHLCY_001310"
     CDS             30371..30766
                     /gene="frdC"
                     /locus_tag="ACHLCY_001310"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0368607.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30777..31136
                     /gene="frdD"
                     /locus_tag="ACHLCY_001311"
     CDS             30777..31136
                     /gene="frdD"
                     /locus_tag="ACHLCY_001311"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0368608.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            31199..32332
                     /gene="ampC"
                     /locus_tag="ACHLCY_001312"
     CDS             31199..32332
                     /gene="ampC"
                     /locus_tag="ACHLCY_001312"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0368609.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32421..32954
                     /gene="blc"
                     /locus_tag="ACHLCY_001313"
     CDS             32421..32954
                     /gene="blc"
                     /locus_tag="ACHLCY_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0368610.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32951..33268)
                     /gene="sugE"
                     /locus_tag="ACHLCY_001314"
     CDS             complement(32951..33268)
                     /gene="sugE"
                     /locus_tag="ACHLCY_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0368611.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33450..33596)
                     /gene="ecnB"
                     /locus_tag="ACHLCY_001315"
     CDS             complement(33450..33596)
                     /gene="ecnB"
                     /locus_tag="ACHLCY_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0368612.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33707..33832)
                     /gene="ecnA"
                     /locus_tag="ACHLCY_001316"
     CDS             complement(33707..33832)
                     /gene="ecnA"
                     /locus_tag="ACHLCY_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0368613.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33884..34450)
                     /gene="efp"
                     /locus_tag="ACHLCY_001317"
     CDS             complement(33884..34450)
                     /gene="efp"
                     /locus_tag="ACHLCY_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0368614.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34492..35520
                     /gene="epmB"
                     /locus_tag="ACHLCY_001318"
     CDS             34492..35520
                     /gene="epmB"
                     /locus_tag="ACHLCY_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0368615.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            35910..36779
                     /gene="yjeJ"
                     /locus_tag="ACHLCY_001319"
     CDS             35910..36779
                     /gene="yjeJ"
                     /locus_tag="ACHLCY_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0368616.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(36983..37336)
                     /gene="yjeI"
                     /locus_tag="ACHLCY_001320"
     CDS             complement(36983..37336)
                     /gene="yjeI"
                     /locus_tag="ACHLCY_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP0368617.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(37474..39120)
                     /gene="groL"
                     /locus_tag="ACHLCY_001321"
     CDS             complement(37474..39120)
                     /gene="groL"
                     /locus_tag="ACHLCY_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP0368618.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(39164..39457)
                     /gene="groS"
                     /locus_tag="ACHLCY_001322"
     CDS             complement(39164..39457)
                     /gene="groS"
                     /locus_tag="ACHLCY_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP0368619.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            39733..40989
                     /gene="yjeH"
                     /locus_tag="ACHLCY_001323"
     CDS             39733..40989
                     /gene="yjeH"
                     /locus_tag="ACHLCY_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP0368620.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(41005..41481)
                     /gene="fxsA"
                     /locus_tag="ACHLCY_001324"
     CDS             complement(41005..41481)
                     /gene="fxsA"
                     /locus_tag="ACHLCY_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP0368621.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            41818..43254
                     /gene="aspA"
                     /locus_tag="ACHLCY_001325"
     CDS             41818..43254
                     /gene="aspA"
                     /locus_tag="ACHLCY_001325"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP0368622.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            43372..44673
                     /gene="dcuA"
                     /locus_tag="ACHLCY_001326"
     CDS             43372..44673
                     /gene="dcuA"
                     /locus_tag="ACHLCY_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP0368623.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            44789..45127
                     /gene="cutA"
                     /locus_tag="ACHLCY_001327"
     CDS             44789..45127
                     /gene="cutA"
                     /locus_tag="ACHLCY_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP0368624.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            45103..46800
                     /gene="dsbD"
                     /locus_tag="ACHLCY_001328"
     CDS             45103..46800
                     /gene="dsbD"
                     /locus_tag="ACHLCY_001328"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP0368625.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEVKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            46837..47412
                     /gene="yjdC"
                     /locus_tag="ACHLCY_001329"
     CDS             46837..47412
                     /gene="yjdC"
                     /locus_tag="ACHLCY_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0368626.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            47519..47594
                     /locus_tag="ACHLCY_001330"
     tRNA            47519..47594
                     /locus_tag="ACHLCY_001330"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47552..47554,aa:Phe,seq:gaa)
     gene            47792..49057
                     /locus_tag="ACHLCY_001331"
     CDS             47792..49057
                     /locus_tag="ACHLCY_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0368627.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGGDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANRERFISPFEYAKINNPLKQ"
BASE COUNT        11795 a        13259 c        12354 g        11725 t
ORIGIN      
        1 taataaaatg aatatttgca gtacgtaatt atcttaccag ctatagacaa aaaaaaacca
       61 tccaaatctg gatggctttt cataattcag acgaactagc tgcgctgacg aaccgcttca
      121 aataagcaaa ttccggttgc aaccgaaacg ttcagggaag aaacacttcc tgccattggg
      181 atgcagatca actcatcgca atgttcacgg gtcaggcgac gcataccttc accttccgcg
      241 cccatcacca gcgccaggcg tccggtcatt ttgctctgat agagtgtatg atccgcctcg
      301 cctgccgtac cgacgatcca gatattctct tcctgcaaca tacgcatggt gcgcgcaagg
      361 ttagtcaccc gaatcagtgg aacgctttct gctgcgccgc aggctacttt tttcgccgta
      421 gcgttgagct gtgcggagcg atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc
      481 gcgctacgca ggcacgcgcc gaggttgtgc ggatcggtta caccgtcgag gatcagcagg
      541 aacggttgat cgagcgaagc gatcagatcc ggcagatcgt tttcctgata ctgacgtcct
      601 ggcttcacgc gggcgataat gccctgatgc acggcaccgt cgcttttctc gtcgagatat
      661 tggcggtttg ccaactggat aaccacgccc tgagactcaa gggcgtgaat cagcggtaac
      721 agacgtttat cttcacggcc ttttaaaata aagacttcct gaaaacgttc aggggcgcgc
      781 tccagcaggg cctgcactgc gtggatgccg taaatcattt cgctcattaa tgtactcgtt
      841 gtttacgttt tgcctgatgc actacgttta tccggcctac atgatctctg caatatattg
      901 aatttgcgtg attatgtagg ccggataagg cgttcacgcc gcatccggca tgaacaaagc
      961 gcattttgtc agcaatctaa ccctcttctt ttaaagaggg tattgatcac tctgccactt
     1021 ttttcttcgc cgctttcggc ttcgtttttt tctcaccgcg gaaggcgctg tctggctcaa
     1081 agtttacctt tttaccgacc tgacgacgct tgccgccttt tttgcctgca tcgccttttt
     1141 tcgctttctc gcgcgccgtt ttaccgacgt tgcgcggtgc gcgttcactg gagatcagac
     1201 taaagtcgat tttgcgctcg tccatattaa ccgcttcgac tcgaacttcc acgcgatcgc
     1261 ccaggcgata agtctggccg ctggattccc ccatcaggcg ttgccctacc tggtcaaagc
     1321 gatagtagtc attgtccagc gaagagacat ggaccagacc atcaatgaac aagtcgtcca
     1381 gacggacgaa gaagccaaag ccagtgacgc tggaaattac gcctttaaag acgttaccta
     1441 cctggtcgag catgaagtca cacttcagcc agtcagcgac atcgcgcgtt gcttcgtcgg
     1501 cacgacgttc cgccatcgaa cagtgctgac ccagttgcaa catctcttcc atcgaataat
     1561 ggtagccgcc ggtttcagtg gtgttgccct gatgcccctg ctctttcgcc agcagatatt
     1621 taatggcgcg gtgcagcgtc aggtcaggat aacgacgaat cggcgaagta aagtgcgcat
     1681 aggactgcaa tgccagaccg aagtgaccac ggttttctgg atcgtaaatc gcctgtttca
     1741 tcgagcgtag cagcatggtt tgcagcattt ctgcatcagg acggtcggca accgactcca
     1801 gcagttccgc gtagtcacgc ggttccggct tgttaccacc cggcagctcc agccccagct
     1861 ccgccagcac tgaacggaaa gaggtaatcg cttcggtgct cggcttgtcg tgaatacgga
     1921 acagtgccgg ttctttcgct ttctcaacga aacgcgccgc cgagatattc gccagaatca
     1981 tgcactcttc aattaatttg tgcgcgtcgt tacgctgggt ctgttcgata cgttcaatac
     2041 ggcgttcagc gttgaaaatg aacttcgctt cttcgctctc aaatgagatc ccaccgcgtt
     2101 cttcacgggc tttatccagc actttataga ggttatgcaa ctcttcgaga tgcttaacca
     2161 gcggggcgta ctgctcgcgc agatcctgat cgccctgcag aatatgccag actttggtgt
     2221 aggtcagacg cgcgtgagag ctcatcaccg cttcgtagaa tttgtagccc gtcaggcggc
     2281 ctttcgacga aaccgtcatc tcgcacacca tacacaggcg gtctacctgc gggttgagcg
     2341 aacacaggcc gttagagagc acttccggca gcatcgggat aacctgcgaa gggaagtaca
     2401 ccgacgtgcc acggttacgc gcttctctgt ccagcggcgt tggcggacgc acatagtagc
     2461 tgacgtcggc aatcgcgacc cataaacgcc agccgccgcc gcgttttttc tcgcagtaaa
     2521 ctgcatcgtc aaagtcacgg gcgtcttcgc catcaatggt gaccagcggt aaatcgcgca
     2581 gatcaacacg gcccgctttt gcttcttccg gcacttcttc tttcagcccg gcaacctgtt
     2641 gctcaacagc ctgcggccag atgtacggaa tttcatgggt acgcagagcg atatcaaccg
     2701 ccatgccggt gcccatattg tcgcccagca cttcgacgat tttacccacc gctttggtgc
     2761 ggcgagtcgg acgctgagtc agttcgacta cgaccacaaa gcccatccgc gcgcccatga
     2821 tctgatcggg cgggattaag atatcgaagc tcagacggct atcgtcagga accacaaagc
     2881 cgacgcccgc ttcagtaaag tagcgaccaa caatctggct ggtttttggc accagtacgc
     2941 ggacaatacg cgcttcacga cgacctttac ggtcagcgcc cagcggctgc gccagcacct
     3001 gatcgccatg aatgcaggtt ttcatctgct cgctggagag atacaaatca tctttacgcc
     3061 cttcaacccg cagaaagccg tagccatcac ggtggccaat aacggtacct ttcaccaggt
     3121 cgaggcgttc cggcagcgca tagcactgac gacgagtgaa gaccagttga ccatcgcgct
     3181 ccatcgcgcg caggcggcga cgcaggcctt caagctgctc ttcgccttca atgtgcagtt
     3241 ctaccgccag ctcatcacgg ctggccggtt tttcacgttt ggttaaatgt tcgaggataa
     3301 attcccgact agggatggga ttcgcgtatt tttcagcttc gcgttcctgg aaaggatctt
     3361 gtgacatctc ggttcctccg ttgtcatctc tgatgaagat tttcgtcact ccaccagcaa
     3421 taatttataa agcggttgat tctcttcaac caaatcggca agcgtgtagt tatccagttc
     3481 cgtaagaaaa ctttgcacgg ccttagaaag cgcctgtttc aaccgacagg caggtgtaat
     3541 gtggcaaaac tcactgctgc aattcaccag cgataagggc tccagctcgc gcaccacatc
     3601 accaatacgt atcgcactcg ccggtttacc caggcgaatg ccgccatttt ttccacgaac
     3661 agcagtcacg tagccggcac gactaagttg attgattatt ttgaccatat gattacggga
     3721 gacgccgtag acgtcagtca cttcagaaat actggtcatc cgcccttctg gcaatgacgc
     3781 catgtagatc agcgcacgta atccgtaatc agtgaaactc gttaactgca catcaacctc
     3841 aaaagggaaa tcgggaaaaa atacatttat attgatgata aaccagccac aagctgtgtc
     3901 gctaatttat ttcagttcgg ggaaggaaaa aagcgaggat ttaacactgt gtcggatagc
     3961 gtaatggctt aaccgacaca gcaatgtatc acactgttag cccggcaggc aaaatatctg
     4021 ccaggcgtac cagaattacg cgtcgaacgg atcgcgcaga atcatggttt cagtacgatc
     4081 cggaccggta gagatgatat cgatcggcac accagtcagc tcttcaatac gcttgatgta
     4141 gttcagcgcc gcctgcggca gaccgctacg atctttcacg ccgaaggtgg attcagacca
     4201 gcccggcatg gtttcgtaaa tcggctctac acctttccag tcgtcagctg ccagcggagt
     4261 ggtcgtcact tcacgaccat ccggcatacg gtaagccaca cagagtttca cctctttcag
     4321 gccatccaga acgtccagtt tagtcaggca gaagccagac agggagttca gctgtaccgc
     4381 acgacgaacg gcaacggtgt ccagccagcc ggtacgacga cgacgccccg tagttgcgcc
     4441 gaattcgtta ccctgcttgc agaggaactc gccagtttca tcaaacagtt cggtcgggaa
     4501 cggacctgca cctacacgag ttgagtaagc tttgaggata cccagaacat aatcaacata
     4561 acgcgggccc aggccggaac cggtcgccac gccaccagca gtggtgttgg aagaagttac
     4621 gtacggataa gtaccgtggt cgatatccag cagcgtaccc tgcgcacctt caaacatgac
     4681 gaaatcgcca cgctgacgcg cctggtcgag caggtcagaa acgtcaacca ccatagaagt
     4741 caggatgtcg gcaacagcca tcgtatcatc cagaactttc tggtaatcaa ccgcttcagc
     4801 tttgtagtag ttaaccaact ggaagttgtg atattccatc acttctttca gtttttcagc
     4861 gaaggtttct ttgtcgaaaa ggtcgccaac acgcagaccg cgacgtgcta ctttatcttc
     4921 ataagcaggc ccgataccac gaccggtggt gccgatcgct ttcgcgccac gcgctttctc
     4981 acgcgcgtta tccagcgcaa cgtgataatc aaggatcagc ggacatgctt cagacagcag
     5041 cagacgctca cgaacgggga tgccacggtc ttccagttct ttcatctctt tcatcagcgc
     5101 ggccggagac agcacaacac cgttaccgat gatgctggtt acattctcgc ggagaatacc
     5161 tgatggaata agatggagaa cggttttttc accgttgatt acgagagtat ggcctgcgtt
     5221 gtgaccgccc tggtagcgta caacatattt agcccgttca gtcagaagat cgacgatctt
     5281 acctttacct tcgtcacccc attgggtgcc cagtacgacg acgttgttac ccatttttca
     5341 aaatcaccgt ttgcttaaaa atggattcta ccatcgcttt ttcagagtta cagcactttt
     5401 tgcactcaaa aatgacctcg atcagtctgt tttttgttca gccaatcgtt ttcctcaaca
     5461 tgtagtagac cacaacgccc gcaaccacaa gtccaccgcc aaaacgacgt aaaatattat
     5521 cgggcaaatt ggtcatcgca gagatcatct tcttccatgc cttcgggtaa agcatcggcc
     5581 ctaaaccttc cagtaccaaa accagggcaa gcgccagcca gattgtcgaa ttcattcatt
     5641 atccttatag aaaaagaaaa ccaccgacat ccctgaggat gcggtggctt tattgacctg
     5701 taccgcagtc gttatattaa cgcgttgcgg aagtcggcgt cttcatgtag cggaagaaat
     5761 cgctatccgg gctcatgacc atcacgtcct gattgccaga gaagctgttc tcataagcac
     5821 gcaggctacg gatgaatgcg tagaagtccg gatctttact gaatgcatca gcaaacagtt
     5881 tggctgcttc ggcatcgcct tcaccacgca tgatgcggcc ctgacgctca gcttctgcca
     5941 gcgttctggt cacttcatag tcggcagtcg cgcgcagttt ttccgcttct tcctgacctt
     6001 gtgaacggtg acgacgcgct accgcttcac gctcggcgcg catacggttg tagatcgctt
     6061 cagacacttc ggtcggcagg ttgatctgct tgatacgcac atcgacaact tcaataccca
     6121 gcgccgccat actgttcggg ttgatgaccg gaactttgcc cttcgtctca gccgttacgc
     6181 gctctgccgc ttcggcaatg gcgttatctg ctgccggggt agtaacttca tcttctgtac
     6241 ccgcagaacc ggagttcagc gcgtcacgta cttcgagggt cagacgacca cgggaatcag
     6301 tgacgatatc tttcacgtcc aggcgaccaa tttcagaacg cagacggtca gagaacttac
     6361 gtttcaacag cacttccgct tgcgaaatgt cgccaccacc cgttgccagg tagtaacggc
     6421 tgaaatcgct gatgcgccat ttgatgtaag aatcgacgat caggtctttc ttctctttgg
     6481 tcacaaagcg gtcggcctgg ttgtccatgg tctgaatacg tgcgtcgagc attttcaccg
     6541 tttcaatgaa cggtatcttg aaatgcagac ccggctcata aaccagaggt ttgttgtcat
     6601 cgtcacgcag taccttacca aaacgcagcg taataccgcg ctcaccttct ttgacgacaa
     6661 agacagacat gtaaagcact accagcacga tgatgataat cgcgataact gacttacgca
     6721 tcgttattcc ccctgacgct ggtagtcgtt acgctgcgcg ttggcgcggc gttggtccat
     6781 aatatcgccc tgactggtgg acgacgtgtt gcttgctcca ctggttgtgg aggaagaggc
     6841 tggcggcaga cgcagcagat tgctggcacc gttatcgctc ttcgccgcag gggcgttacc
     6901 acctttcagc atctggtcta acggcagaac catcaggttg ccacctttgt cgttaaccag
     6961 cactttgcgg gtgttgccca acactttttc catcgtctcg atatacagac gctcgcgagt
     7021 aatttccggc gcggctttat attccggcag aagtttagca aagcgcgcca cttcaccctg
     7081 agcttccagg atggtctggg ccttgtacgc acgcgcctct tcgaggatac gttgcgcctg
     7141 accgttcgca cgcggctgaa cttcgttggt atacgcttct gcttcacgaa tgtattgctg
     7201 ttcgttttca cgcgcggcaa tcgcatcgtc aaacgccgct tttacttctt ccggcggacg
     7261 agcagcctgg aagttgacgt ccagcagcgt gatacccatg tcatacggac gaatcgtctc
     7321 ttccagttcg cgctgagtat cgctacgaat cacggtacga ccttccgtca gaatgcggtc
     7381 catggtgtat ttaccgataa ctccacgcag ggcgctgtcg gtagcctgac gcaggctgtc
     7441 atccgggctg gtcacgctat acagatattt ttccggattg gtgacgcggt actgcacgtt
     7501 catctcaacg cgcactacgt tctcgtccga cgtcagcatc acaccagaag cggccagttc
     7561 acgcacggct tccacgttca ccggtttgac ttcgtcgata aacgtcggtt tccagttcag
     7621 acccggctca accagatggc tgaatttacc aaagcgtgtt accacgccgc gttcggcttc
     7681 tttaatggta tagaaaccac tggccgccca gataatgaca atcgctgccg ctgcgatggt
     7741 aacgacacga ccgccaagct gcgggcgcgg gccttgcgat gaactgccac cgccagatcc
     7801 ggtgccttta ccgccgccca gaccaccgag ctttttgctc agtttgcgga agatatcatc
     7861 taaatcaggt ggcccttgat cgcgaccgcc tttgtttcca tttccctcag agttgccgcc
     7921 aggtttgctg cttccccacg ggtcgcggtc ttgtccgtta ttaccgggct gattccacgc
     7981 catgtttgtg ctccatattt gttatgcggt gatccctgtt gtctttcgac gccagaggat
     8041 atgactccac gcttcagacg ctacgccgtt agatcaggta atcgatcaac gccggttctt
     8101 gtttacagag gcgacgccag tcaacgatcg gcatacgaac ttgcagactt acgctgccgt
     8161 cctcctccat ccactctttt tctattgcct gaagctgata aaaacgactt ctcagacgcc
     8221 cttcctgcgg tggcagacgc aatgtatgct gcgccacctc gccggaaagc cgctccgtca
     8281 aagcctgaaa aagctgtggt atccccgctc cggtctgtgc ggaaagccag acacggatcg
     8341 gtttgttctc ttcgtcccga tcaatacgcg gttcgaaatc ttccagcata tcgatcttgt
     8401 tcatcaccag cagggttggg atctcgtgag cgtcgatctc ttcaagaacc gtattcaccg
     8461 cttcgatgtt ttcttgtaca cgcacatccg ccgcatcaat gacgtgcagc agtaatgtgg
     8521 cttgccgcgt ctcttgtaac gtggctttaa atgccgccac cagatcgtgc ggcaggtggc
     8581 gaataaaccc tacggtatct gcaagtacgg tttcaccgac atctgcaacg tcaatacgcc
     8641 gcaacgtcgg gtcgagggtg gcaaacaact ggtctgccgc gtagacccgc gcttcggtga
     8701 tgcgattgaa aagggtagat ttaccggcgt tggtatatcc caccagcgaa acagtaggaa
     8761 cgtcggcttt gatacgcgat tgccgcccct gctcacgctg cttttcaact ctttccaggc
     8821 gtgactgtat ctgcacgatg cgattacgca acaaacgacg gtcggtttcg agctgggttt
     8881 cacccggacc acgcaaacct atcccgcctt tctgtctttc aaggtgggtc cagccacgca
     8941 ccaggcgcgt agccagatgg cgcagctgcg ccagctcaac ctgcaactta ccctcatggg
     9001 tacgcgcacg ttgggcgaaa atatctaaaa taaggccggt gcggtcgata acacgacact
     9061 cgcacaaacg ctccaggtta cgctcttgcg ccgggctcag ggcatggtca aaaagaacga
     9121 ccaaagcacc cgtcgctttg acagcttccg caatttcaac tgctttacct tcacctacaa
     9181 aatactttgg gtgcggcgct ttacggctac cggtaatcac ctgcaatgct tcgacaccgg
     9241 cggaagagac cagagattca aactcctgga ggtcttccat atctttgtct tgcgtaaaat
     9301 agatgtgtac cagtaccgcc tgctcaccag catcataacg gtcaaacaag cgtataaccc
     9361 tctaaataga tcagcgggga acgcaggatc gctggctccc cgtgtaaaaa aaacagcccg
     9421 aaaccttatt cggtttcttc gctgtcctgt tgcgcggaag tattctgcgc gctgctacca
     9481 tgatggtagt tactgctggt accgccaccg gcgttgttac tgtgatgaga aaccgggcga
     9541 gacgggacaa cagtagaaat cgcgtgcttg taaaccatct ggctgaccgt gtttttcaac
     9601 aggatcacga attgatcaaa agactcgatt tgcccttgca gcttaatacc attcaccaaa
     9661 taaatagaaa ctggaacacg ttcccgacgc agtgcgttca ggaacggatc ttgtaaagat
     9721 tgccccttag ccattctctc ttttccttat atgcttattt gtactttgaa cctttcgatt
     9781 ctgaaaaaat tgcgcacgat acgtctcaat tgtacacatt cagcctgcga tagcaccaac
     9841 aacctgtaat acttcgtcgc gcgcctgttc tggtttttca ctgtcaagcc agtgaacccc
     9901 ttcccaacca cgcagccagg ttatctgccg cttcgccaac tgtctcgtgg cgcaaacacc
     9961 tcgataaacc atttcatcgt atgagatttc gccttcaagg taagaccaca tctggcgata
    10021 acccacgcaa cgaatggaag gcaagtccgt atgcaaatct cctcgggcaa aaagcgcccg
    10081 gacttctgct tcaaaacctg aagccaacat ctgatgaaaa cgctgctcaa tgcgttgatg
    10141 gagcagttca cggctcgccg gggcgatggc gaactgatgc acctgatacg gtagagcgtc
    10201 tcctgacgtt tgcgtcagtt ccgttaaagt tttacccgaa atgaaaaaaa cttccagtgc
    10261 ccgggaaagc ctttgtggat catttggatg aatccttgct gccgcaaccg gatctacctc
    10321 ctgaagttga cgatgcaatg actcccaacc ttgctctacc gcctgttgct caattctggc
    10381 ccgcacttcc gggtctgccg acggtagcgg cgacaaccct tccagcaatg ccttgaaata
    10441 caacatcgta ccgcccacta acagtgggat ccgccccgcc gcggtgatat cggccatttc
    10501 cgccagcgca tcgcggcgaa aatcagcagc cgagtaagcc tgcgacggat cgcgaatatc
    10561 cagcaatcgg tgcggcgcgg cgagtaactc ttcagcgttc ggcttcgccg tcccgatatc
    10621 catccctttg taaataaggg cagaatcaac gcttatcaac tctactggta aaattttacg
    10681 cagctcaatg gctaacgccg ttttaccgga ggccgtcggc cccatcaaaa aaatcgcctt
    10741 aggcaggctc gccttactga tatcactcat ctttcagggc ttttatcgcc ggatgtaaat
    10801 caacagattg taacagacca cccggcggcg ttttcacaag ttgcggacat aaccgttcca
    10861 cgtccgccag cagggttatg gcctgtgcca ttgaccactg cgcatgttcg ctcatcagat
    10921 ttcgtgcaat ccactgcgca atattgccag gttcgaatac ggactgcttc gccaggtagc
    10981 ctatcagttc aggaatcaag atttgtaaat tttgttggcg taagggtaaa ggcacggccc
    11041 tgatggtcac atgctgtgca tctgactgga aatcaatacc caattccgcc agggcagact
    11101 gcgctttttc taatgccgat ttttcttcgc cagaaacttt tagccgcaac ggaatcagca
    11161 acggctgggc gcaaactggc gcttcacccg gcgtcagttg cgcctgacgc agccaacgtt
    11221 ctgccactgg caaggctaaa agtgaaatgt tgccatcgcg ctccagcaac gcacagtcgg
    11281 aatggacgat agtcagtacc cgaccaaaac tctgactgtt cgccgcaagt gcaggttcct
    11341 gcggttccgg cgcttttggt ttttgcatcg gcgcgggcgt ttgcaaaagc tggcgataca
    11401 cttcaccttg ctgtttctgg tagcctggct gcgcattcgg ccagggggca gccggacgac
    11461 tgcctgaggc gggcgcagga gagtagcgcg gagctaccgg ctcacgagct gccggttctg
    11521 caaagtgatt gcgccccgcc gccacgcggt tttccggaat gggacgcggt gcaggttggg
    11581 gttcatcgtc cagcggtagc ggcgtttcca gttgctgttg tagcacgctc agcacgccct
    11641 gatagataaa atcatgcacc agacgcgact gatggaaacg cacttcgtgt ttggcggggt
    11701 gtacgttgac gtccacctga tgcgggtcga tctccagata caacacaaac gccggttgtt
    11761 gatcggcccc cagtttgtct tcgcaggcct ggcggatcgc atgattgatc aggcgatcgc
    11821 gcatcatgcg accattcacg tagcaatact gaatttccgc cagtgcgggc gtagtgtgat
    11881 ttggatcggc cacccagccg cgcagcgtga gatcgccgtg ttgccattca atcgccagcg
    11941 cttgttcgag aaaagcggtg ccacaaatcg cgcctaagcg ccgttctttt tgcccgcctt
    12001 ccggcactgc gcggtactga cgcacaattt taccgttatg cgacaggttg atcgtgacgt
    12061 cgaaacgcgc cagcgcaatg cggcggatga tttcatcaat atggctaaat tcggttttct
    12121 cggtgcgcag gaatttgcgc cgcgccgggg tgttgtagaa cagatccagc acctccagcg
    12181 tcgtccccac aggatgcgcc gccggtttta ccgtcacgtt catatcgcgc ccttcggcat
    12241 aggcctgcca ggcttcctgc tgttctgcgg tgcgtgaagt gagcgtcagg cgggaaaccg
    12301 aactgatact cgccagcgcc tcaccgcgaa agcccaggct gataatggct tcgagatcgt
    12361 ccagagaggc gattttactg gtggcatgac gagccagcgc cagcgccagc tcatcttttt
    12421 tgataccgca gccgttatca cgaatgcgga taagtttcgc cccaccgcgt tcgatatcaa
    12481 tatcgatacg cgtcgcacct gcatcgaggc tgttttccac cagttctttg actaccgacg
    12541 caggtcgctc gaccacctca cctgcggcaa tctggttcgc cagttgtggc ggtaagacct
    12601 gaattggcat caatcgtcct tagtttggca gcgtgcgatc tggcgtcgtc accgtactgg
    12661 cagtttgtgc cgttgccccc tgcggcgcag attgcatcgg atgcgcaagg aaataattgc
    12721 gcaggccttt gtaaatggct tctgccagct gttgttggta atcgtcgctc gccagcaaac
    12781 gttcttcgct gttgttgctg ataaaaccgg tttcgaccag tactgacggg atatccggcg
    12841 aacgcagaac gccgagactc gcgtgttctg gtcggcgttt atgtatttcg ccaatgcgtt
    12901 gcaactgact gatcatactg gtcgctacat cataccctac ccgttgggaa tgaccgaact
    12961 gtaaatccag caccgcctgg cttaaatagg ggtcagactg actgttcgcc agcacatcac
    13021 ccgccccacc cagcagctcc gactgtttct cgtgctgctc cagccagctg gccatttcgc
    13081 tgttggcgcg acggttagag agcacccata cggaagcgcc agtcgcactg cggttcggtg
    13141 cggcatcagc gtgaatcgac acgaggaaat tggcgttttg cttacgtgcc acatcactgc
    13201 gccccatcac cgagataaag taatccccgt cacgggttaa aacgccctta aacatcggat
    13261 cgtcattgag caaagtacgc aatttacgcg cgatggcgat ggtgacattt ttctcccgcg
    13321 taccaccggg gccgatagcg ccagggtcct gaccgccgtg tccggcatca atagcgatga
    13381 taattttatc gccagtgtta gccgtcgcgc gcgctgccgg acgcgttacc gtattactgc
    13441 tgataacacc cgtagtgcgg ttactttccg ttttaaacgg attgcgcgcc ggttcgctga
    13501 cgcgcggtgc gacaaccgca ggcgtttcaa cgcgtttcgc aaccacgggc ggcggaggag
    13561 gaggtggcgg cgcatcggcg ttaatcgtaa agacgacagt gtaattgctg ccattctgcc
    13621 gcttcaccgc ttcggtttta ccgttttcgg taagatcgac caccagccgt agcgtttgtg
    13681 catctttagg cgttccagag cgaatcgcct tcaccagatt attgccgctg aacaacaacg
    13741 gcagtccctg aatcacgccc gtttgtttga tatcgagcgc cacggtgcgt ttgctttgat
    13801 ggctaaacgc ataatcagga tcgccaataa aactcaacgt tatccgcgcc tgttgattac
    13861 cgttagaaac ctgaatatca gagagcgtcg cggcacccac cggcgtgcac agcagcagca
    13921 gcgtcgctac caaccaattt ctgatgcgat acatcatccc gccacctttc aaaggttaac
    13981 cggctaaacg cgccagcaac aattcacccg cagaggaaac cgcactcacg cgcgcctcac
    14041 ggccttgtgc ctgataatca atgtgtattt cgacatccgg gtcaggaaga acacctgtac
    14101 cttgttgtgg ccactccacc aggcagatgg catcgttggc aaaataatcg cggatcccca
    14161 taaactccag ctcctcggga tcggcaaggc ggtacaaatc aaagtgataa accattaaat
    14221 tgtcgagcgt atagggttcg accagcgtat aagtggggct tttgacatta ccctgatgac
    14281 ccagagcttg taaaaagccc cggctaaagg tggttttacc tgcgcctaaa tcgccataca
    14341 gatagattac ggttgcgcca tcgcaggctt tcgctacccg ctcgcccagg tctaatgttg
    14401 cctgctcatc agggagcgga attactcgat tcatcatggt ttttatcagt cacttccggg
    14461 ttaacaatac gctgtagcgt ggaaaagaga tcggttgcca gcatcccgcg cgttccaaaa
    14521 cgcgccgcca gtacgtcagc tgcagcaccg tgcgcgacac agcccgcaca ggcggcatca
    14581 tacggcgaca gtttttgccc aagcaatgcg ccaataatac cagagagcac atcgcccatg
    14641 ccgccgctcg ccatgcctgc atttccggca tcaataatgc ctaaagcgtc aggatgggcg
    14701 gcgaccacgg ttccggcacc tttcagcacc gctacgccgc cataacgttg taccagacgt
    14761 ttggcgcaat gtaagcggtc actttcaatt tcagcgacgg aacagcctaa caaccgtgcg
    14821 gcctcgccag gatgcggcgt gatcacgcga ttgtgacgct tatcgggatt gattgccagc
    14881 aggttcaatg catcggcatc ccacaacatc ggtttgcgaa aattctcaac tttttgcagt
    14941 gcttttttcc cccactcttg ctggcccaga ccgggaccaa tcaccaccac atcggcccat
    15001 tccaggcttt cggtaagaga gtccatcgtc agttcatgca ccatcaattc cggtcgtgca
    15061 gtcagcagcg gcgcaatgtt ttcactgcgg gtcagtactc ggactaaacc agcaccagca
    15121 cgtagcgccg cttcccccgt catacgaata gcccccgccg tgccgtgatc gccaccgata
    15181 attaccagcc gcccgtgatc gcctttatgc gaagtcgggc gacgcggttt tagccagtga
    15241 gaaagttgtt ctgccgaaaa ccgctgaatt ttcgtctcct gacctgccag ccaactatcc
    15301 agccccagtg agtcaaaatg cagttgtccg gtaacatccc gcgcttttcc agtgagcaag
    15361 cctggtttca gcgcaataaa agtgatggtg tgatcggcgt tgatcactgc gcctggcgta
    15421 gcgccggttt cagccagcag gccggaaggg atatcaaccg ccgcaatcgg cgcaggatgg
    15481 gaattagcgt ggtcgattaa ctggctaatg gattcgcggg gcgcttgctg caagccggta
    15541 ccgagcagcg catcaacaat cagatctacc gattcgggcc agacaatatt cgaagcatgg
    15601 atctcgcctc ccgcgtttaa ccatgcttcg cgtgccagcg cggcctcttc cggcaacggt
    15661 ttgtcgctct cctgggccaa caacgtgacc tcaatgccga ccgctgtggc cagtcgcgcg
    15721 accacgtagc catcgccgcc gttattacca tgaccgcaca gcaccagcca gtggcgggcg
    15781 tcaggatacg ccgaacgaca cacctggaat gcggcttcgc cagcgcgaag catcagctca
    15841 tagagtgtaa gccccagcgc atctgccgcc tcacgttctc cgcggcggat atcgtcggcg
    15901 tgccagacgg tgtgtggtat acttacgggg tttttcttca ttgtatggtc cgtcatgtca
    15961 gagcccctcg atctcaatca gttagcgcaa aaaattaaac agtgggggct ggaactgggc
    16021 tttctgcagg taggtattac cgataccgat ctcagcgagt ccgagcccaa actgcaagca
    16081 tggctggaca aacaatacca cggcgaaatg gactggatgg cacgtcacgg tatgctgcgc
    16141 gctcgccccc atgagttatt acccggtacg ctgcgcgtga tcagcgtgcg gatgaattac
    16201 cttcctgcta acgccgcatt tgccagcacg ctgaaaaacc ccaaactcgg ctatgttagc
    16261 cgttatgcgc tgggccgtga ctatcacaaa cttctgcgca accgactcaa aaagctgggc
    16321 gagatgattc agcaacattg tgtttcgctg aattttagac cgtttgtcga ttctgcgcct
    16381 attctcgagc gcccgttagc tgcaaaagct gggctcggct ggacaggtaa gcactcactt
    16441 atcctcaatc gcgaggccgg ttctttcttc tttttaggcg aattgctggt cgatattccg
    16501 ctgcccgtgg atcaaccagt cgaggaagga tgcggtaaat gcatagcctg tatgacgatt
    16561 tgcccgaccg gtgccatcgt cgagccatat accgtcgatg ctcgccgctg tatctcttat
    16621 ctcaccatcg aactggaagg ggcgatcccg gaagagttgc gaccgttaat gggaaaccgt
    16681 atttacggtt gcgatgactg ccagcttatc tgcccgtgga atcgctattc gcaactcact
    16741 acagaagacg atttcagccc gcgtaagccg ctacacgcac cggaactcat tgagttattc
    16801 gcctggagcg aagagaagtt tttaaaagtc acggaaggat cggcgattcg ccgtataggt
    16861 cacctgcgct ggctgcgtaa tatcgccgtg gcattaggca atgccccctg ggatgaaacg
    16921 attctggcgg cgctggagag tcgcaaaggt gagcacccac ttcttgatga gcacatagcg
    16981 tgggcgatgg cgcagcaaat cgagaggcga aatgcgtgca tagtcgaagt gcaattgccg
    17041 aaaaaacagc gtctggttcg ggtgattgaa aaagggttac cgcgtgacgc ctgattcatc
    17101 cacagcttgt gaataaaaat aaaaacgcat tgcaattcaa gacgaataaa atcgtcaagt
    17161 gatcgactcg acaaaacgaa atcttaattt ttaacttatc tatcaataag ttaaaaaaat
    17221 actattcacc gtgcgaagtt tctttgaaat taagactaca gcctgaggct gtggataact
    17281 ctgtttacaa gagtttgtca gagacaacaa aaaccagcaa taacgcatcg cgtcgctgtg
    17341 gatattttat tgagagaaga atttggagcg ggaaacgaga ctcgaactcg cgaccccgac
    17401 cttggcaagg tcgtgctcta ccaactgagc tattcccgct tgggtggtct gtgccttaca
    17461 gcactttcaa attttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag
    17521 gtcgtgctct accaactgag ctattcccgc ttaatcttcg tctttcgaac cgctactgcc
    17581 ttggctgcct tcactcacct tagtcacata cttttgtaag ttcccaagga ttcgttcagt
    17641 tgccgccttg tcgcaattcg aaatactgcg attggatggt ctgtgctttg cagcactttt
    17701 caaattttgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
    17761 tctaccaact gagctattcc cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc
    17821 tgcgtcgtta cgggaggcgc attatacaag aaatctttcc ctctgcaacc ccccaaaaaa
    17881 gttttttttg cgtttttatc tcaagtgctg aaattatcag caagacgttc aatttagcgt
    17941 caaaatcacg ccgccgcaag tggattccgg cgcttacgtg accaggaaaa attttacagc
    18001 ttgataaaat gctcgcggta gtaagccagc tccgccaccg attcacggat atcatccatc
    18061 gcctgatgcg tcccctgctt ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc
    18121 agctctttca gggtgctgac atcgagataa cggtagtgga agtaggcttc cagctccggc
    18181 atgtatttaa acaggaaacg acggtcctga ccgatgctgt taccgcaaat cggcgatttt
    18241 cccgcaggca cccactgttt taaaaattcg agcgttgcca gttcagcttc ccgatcgccc
    18301 atcgtgctcg ctttcacgcg ctctaccagc ccgctggcgg tatgggtgcg cacgttccag
    18361 tcatccatca gcgccagctg ttcatcagac tggtgtactg caatggtcgg cccttctgcc
    18421 agaatattca ggttggcatc ggtcaccagc gtggcaatct caataatgcg atcgcgctcg
    18481 ggatccagac cggtcatctc aagatcgatc caaatcaggt tgttttcatt ggcactcatg
    18541 ctattttcca cccattcaca gtgactatat tgatccacaa ttgcgtgtat catagatgtt
    18601 ttgcccatca ggggcgacca ggagtctgta cgattgagta aaaataaact ctccaaaggc
    18661 cagcagcgcc gcgtgaacgc caatcaccag cgtcgtctta aaacgtctaa ggagaagccc
    18721 gactacgacg acaatctgtt tggcgagcct gatgaaggta tcgtcatcag ccgctttggt
    18781 atgcacgctg atgtggaatc cgccgatggc gacgttcacc gctgcaatat tcgccgtacc
    18841 atccgttcgc tggtaaccgg cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa
    18901 ggcgtaaatg tcaaagggat cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt
    18961 ccggatttct acgacggcgt aaaacctatt gccgccaaca tcgaccagat tgtcattgtc
    19021 tccgccattt tgccggagct gtcgctcaat attatcgacc gttacctggt ggcctgcgaa
    19081 accttgcaga ttgagccgat tattgtgctc aacaagatag acctgctgga cgacgaaggc
    19141 atggcgttcg tcaacgagca gatggatatc taccgcaata tcggttatcg cgtattgatg
    19201 gtttccagcc atactcagga tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc
    19261 agcatttttg ccgggcagtc tggcgtcggc aaatccagcc tgctgaatgc gttgctgggg
    19321 ctgcaaaaag agatcctgac caacgatgtc tctgacaact cggggctcgg tcagcacacc
    19381 acgaccgccg ctcggctgta tcacttcccg cacggcggtg atgtgattga ttcccctggt
    19441 gtgcgtgagt tcggcctctg gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa
    19501 ttccatgact atttaggtct gtgtaaatat cgcgattgca aacacgatac cgatccgggc
    19561 tgcgctatcc gggaagcggt tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat
    19621 caccgtattc tggaaagcat ggcgcaggta aaaacgcgta aaaacttttc tgatacggat
    19681 gactgacaat taagctaacc gtcgttagaa tcgtcccctt tttttcagga tccggcatgt
    19741 tggccggatc aggaacgaca aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa
    19801 ctttcgctac agtacattct gccgaaacta tggcttactc gcctggcggg ttggggcgca
    19861 agcaagcggg caggatggct gacaaaactg gttatcgatc tgttcgttaa atactacaag
    19921 gtcgacatga aagaggcgca aaagccggac accgccagct accgcacctt taacgaattc
    19981 tttgtccgtc cgctgcgtga cgaagtacgc ccaatcgata ccgatccgaa tgtcctggtc
    20041 atgcctgccg atggcgttat cagccagttg ggtaaaatcg aagaagataa aatcctgcaa
    20101 gccaaaggcc acaactacag cctcgaagcc ctgctggcag gcaactatct gatggcggac
    20161 ctgttccgca acggtacgtt tgtgaccact tacctctccc cgcgtgacta ccatcgcgta
    20221 cacatgccgt gcaacggtat tctgcgtgag atgatctacg tgccgggcga tctcttctcc
    20281 gttaaccatc tcaccgctca gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt
    20341 tgcctgttcg ataccgaatt tggcccgatg gcgcagattc tggtcggggc gacgattgta
    20401 ggcagcattg agacggtctg ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag
    20461 cgctggacct ggcctgccgg ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa
    20521 atgggccgct ttaaactcgg ctctaccgtt atcaacctgt ttgcaccggg taaagtgaat
    20581 ctggttgagc aactggaaag cctgtctgtc acgaaaattg gccagccgct ggcagtatct
    20641 accgaaacct ttgttacgcc agacgctgaa cccgccccac ttcctgctga agagatcgaa
    20701 gcagaacacg acgccagccc attggttgac gataaaaaag accaggtcta atccatcaaa
    20761 ggaaacgctg acgtgcgcct gattatcact tttctgatgg cctggtgcct cagttggggg
    20821 gcgtacgccg cgacggcccc cgatagcaaa caaatcactc aggaactgga gcaggcaaaa
    20881 gcggcgaaac ccgcacagcc ggaagtcgta gaggcgctcc agtctgcctt aaatgcgctt
    20941 gaggaacgaa aaggttccct tgagcgcatc aaacaatatc agcaagttat cgataattat
    21001 ccgaaactct ccgctactct gcgcgcacaa ttaaacaaca tgcgtgacga gccgcgcagc
    21061 gtgtcgccgg gaatgtctac cgacgcgctg aatcaggaaa ttctccaggt cagcagccag
    21121 ttgctggata aaagccgtca ggcccagcaa gagcaggagc gcgcccgcga gattgccgat
    21181 tcgctgaatc aactgccgca acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag
    21241 cgccgcctgg gaacgcttac tggcaatacc ccgctcaatc aggcacaaaa tttcgcgttg
    21301 cagtctgact ccgcacgcct gaaagcgctc gttgatgaac tggaactggc gcagctgtct
    21361 gccaataacc gtcaggaatt agcgcgctta cgctcagagc tggcggaaaa agagagccag
    21421 caactggatg cgtatttgca ggccttacgt aatcagttga acagccaacg tcagcttgag
    21481 gcggagcggg cgctggaaag taccgaattg ctggcagaaa acagcgccga tttgccgaaa
    21541 gacatcgtcg cgcaattcaa aattaaccgc gaactatcgg cggcactgaa tcaacaggcg
    21601 cagcggatgg atctcgttgc ctcgcaacag cgccaggcca ccagccagac gttacaggtt
    21661 cggcaggcgc tgaatacgct gcgtgaacag tcgcaatggc tgggatcgtc caacctgctc
    21721 ggcgaagcgt tgcgggcgca ggtggcacgg ctgccggaaa tgccgaaacc acaacagctt
    21781 gataccgaaa tggcgcagtt gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa
    21841 cagccgctgc tacggcaaat tcatcaggcc gacggtcagc cgctgaccgc cgagcaaaac
    21901 cgtattctgg aagcacagct acgcactcag cgtgagttgc tgaactcatt gttgcagggt
    21961 ggcgacacgc tactgctgga actgaccaag ctgaaagtct ccaacgggca acttgaggat
    22021 gcgctgaaag aggtgaatga agcgacgcat cgctatctgt tctggacctc tgacgtgcgc
    22081 ccgatgacca tcgcctggcc actggaaatc gcccaggatc tgcgtcgtct catttcgctg
    22141 gacaccttca gtcagttggg caaagccagt gtgatgatgc tgaccagcaa agagacgatt
    22201 ttgccgctgt ttggcgcgtt gattctggtc ggttgcagta tttactcgcg ccgctatttc
    22261 acccgttttc ttgaacgttc ggcggcgaaa gtcggcaaag tgactcagga tcacttctgg
    22321 ctgacgttgc gcactctttt ctggtcgatt ctcgtcgcgt caccgctgcc ggtgctgtgg
    22381 atgacgctgg gttacggctt gcgcgaggcg tggccttatc cgctggcggt cgcgattggc
    22441 gatggcgtaa cggccaccgt gccgctgctg tgggtagtga tgatttgcgc cacctttgcc
    22501 cgcccgaacg gcttgtttat cgctcatttt ggctggccgc gcgaacgtgt ttcccgtggg
    22561 atgcgctact acctgatgag catcgggctt attgtgccgc tgattatggc gctgatgatg
    22621 ttcgataacc tcgacgaccg tgaattctcc ggttcgctgg gacggctttg ctttatcctc
    22681 atctgcggtg cgctggcggt ggtcaccctc agcctgaaaa aggccgggat cccgctgtac
    22741 ctcaacaaag agggcagcgg cgacaacatt accaaccata tgctgtggaa catgatgatt
    22801 ggcgcaccac tggttgccat tctggcgtca gcggtgggtt atctggcaac ggcgcaggca
    22861 ctgttagcga ggcttgaaac ctcggttgcc atctggttcc tgctactggt ggtttatcac
    22921 gttatccgcc gctggatgct gatccagcgg cgcaggctgg cgtttgaccg ggcgaagcat
    22981 cgccgggcag agatgttagc gcaacgcgcg cgtggcgaag aggaagcgca tcatcacagt
    23041 agcccggaag gggcaattga agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg
    23101 caatccttgc ggctggtgcg ctcaattttg atgttgatcg ccctgctttc tgtcattgtg
    23161 ctgtggtcag aaatccattc cgctttcggc ttcctcgaaa atatttcgct gtgggatgtc
    23221 acctccacgg tacagggcgt agaaagtctg gagccaatta ccctcggtgc ggtgctgatt
    23281 gccattctgg tgtttatcat caccacgcag ctggtgcgca acctgcccgc gctgctggaa
    23341 ctggcgattt tgcagcacct ggatttaacg ccgggtacgg gctacgccat caccaccatc
    23401 accaaatatc tgctgatgct gattggcggg ctggtcggct tctcgatgat tggtattgag
    23461 tggtcgaaat tgcagtggct ggttgccgcg ctcggtgttg gtctcggttt tggtttgcag
    23521 gagattttcg ccaactttat ctctggcctg attatcctgt tcgaaaaacc gattcgcatt
    23581 ggcgatacgg taacgatccg cgatcttacc ggtagcgtga cgaaaattaa cacccgcgcc
    23641 accaccatca gcgactggga ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc
    23701 gagcagttta tcaactggtc gctctctgac tcggtcacgc gcgtggtgtt gacgataccg
    23761 gcccctgccg atgccaatag cgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc
    23821 tgctcgctgg tgatcgacaa cccggcaccg gaagtcttcc tggtggatct gcaacagggg
    23881 attcagattt tcgagctgcg tatttacgcc gctgagatgg gtcaccgtat gccgctacgc
    23941 catgagatcc accagctgat tctggcaggc ttccacgccc acggtatcga tatgccattc
    24001 ccgcccttcc agatgcgtct ggaaagcctc aacggtaaac aaacggggag aacgctgacg
    24061 tctgcgggca aaggtcgtca ggcgggaagt ttgtaagttt gtgtgccgga tgaaccaatc
    24121 cggctcacaa acaaaactga tagcgcagaa aaaactgcca cagccagtag ccgaaaatag
    24181 ccagcgcggt ggcgtacata aaccagccgc gtttttttcg ccacaaagcg aaccagagaa
    24241 aagggaaaaa gagcggtaac gtcattatca caccaacatc acgggtatcg tcttcttcga
    24301 gatacatcaa cacatcgcag acgttgttaa tcccgcgtcc atcaatcatc cactctttgt
    24361 ccagcgcgga agtgatggtt atccagagga acgccacaat aaaccagatg cagcataaga
    24421 caaacatgcg cgcactcata aggtaaccgg tcgcttaatt tcgctatagc gcatacgaaa
    24481 caaatcgccg tatcccacta attccccgcg aaaaaccaga catcggataa gataagcgca
    24541 ataaatatcc ggcaggtttc cccaccgttt accatcgcga tctttgaggg tgttaatggt
    24601 gctacccacc accatcgcca ctttacgggt gtggcaggca tcgacaaaag agagaatcgt
    24661 ctgctctatt tccgctacat ccccggcaga aaattgcagc gccctgattt catcttcggt
    24721 tattgccggg tcccggctgg tgtcatccat aacatttcct gtctttacgc cctatcgtca
    24781 ggtagacgat agggcatcgg gttattccac cgcccactgc ggattttttg ccactctgct
    24841 gcaatagttc tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat
    24901 ccacaatgtg ctgtcccagt ctccggcttc aaccacaggt tgaatgatgg tgaagacgtt
    24961 cgcaaatgtc accaccagta cgacaaccac tgttgcaatc attgccgaca tatgcgtttt
    25021 gaaaatcaca aatggtctgt cgagatcctg acgtgcttta aaaaatggga aagccagcgc
    25081 gaggaacagg taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa
    25141 cgccgatgcg gtaccgccac caaacgaaac cagcaggatg aagacagtaa ccaacccgca
    25201 ctgcatccac atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg
    25261 ccacaacgct ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa
    25321 gaacgcaccg gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc
    25381 cagcgacaat gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct
    25441 cttcatcagc acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg
    25501 ccagtttgtg ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat
    25561 agcggcaaaa acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag
    25621 accaccaacc gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat
    25681 tgccagaccg gactgataac ctgggttcgg tgatgcaagg aaattaatat cctgcgcgaa
    25741 atgcccacca tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca
    25801 cattactgca ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc
    25861 aacgacggtg accagaatca tccatgccac tgccagcaga ccaaccacct gcgtaggctc
    25921 cagtccagca atacgccagt gctgggtcat gtcgctacca tagaggaatg ttgagaacgg
    25981 tacccaaact ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa
    26041 cgtaccaatg aaggcaaaac gtggtccgac actattattc atccaggaat agataccgcc
    26101 ttcttctttg cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa
    26161 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
    26221 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
    26281 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
    26341 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
    26401 tacctggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
    26461 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
    26521 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
    26581 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
    26641 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
    26701 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgtcaattc atggaaacca
    26761 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
    26821 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
    26881 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcag cgtgtcgcgg
    26941 tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc
    27001 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
    27061 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
    27121 aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga
    27181 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
    27241 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
    27301 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
    27361 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
    27421 cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc
    27481 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
    27541 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
    27601 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
    27661 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
    27721 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
    27781 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
    27841 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
    27901 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
    27961 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
    28021 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
    28081 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
    28141 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
    28201 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
    28261 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
    28321 gtcatgttcg cggcgtggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
    28381 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
    28441 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
    28501 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
    28561 aaggctgccg cggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
    28621 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
    28681 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
    28741 ccacgccgcg tggcgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc
    28801 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
    28861 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
    28921 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
    28981 tgcacggtgc aaaccgtctg ggttctaact ccctggcgga actggtggtc ttcggccgtc
    29041 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
    29101 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
    29161 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
    29221 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
    29281 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
    29341 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
    29401 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
    29461 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt
    29521 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
    29581 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
    29641 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
    29701 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
    29761 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
    29821 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
    29881 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
    29941 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
    30001 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
    30061 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
    30121 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
    30181 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
    30241 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
    30301 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
    30361 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
    30421 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
    30481 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
    30541 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
    30601 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
    30661 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
    30721 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
    30781 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
    30841 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
    30901 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
    30961 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
    31021 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
    31081 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
    31141 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
    31201 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
    31261 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
    31321 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
    31381 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
    31441 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
    31501 aagcgatccc acaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac
    31561 actattacat ctcgcaacct acactgctgg cggcctgcca ttgcaggtgc cggatgaggt
    31621 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggcgccagg
    31681 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
    31741 gtctggtttg agttttgagc aggcgatgca aacacgtgtc ttccagccac tcaaactcaa
    31801 ccatacgtgg attaatgtac cgcccgcaga agaaaagaat tacgcctggg gatatcgcga
    31861 aggcaaggca gttcatgttt cgccaggggc gttagatgct gaaacttatg gtgtgaagtc
    31921 gaccattgaa gatatggcct gctgggtacg aagcaatatg aatccccgtg atatcaacga
    31981 caaaacactt cagcaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
    32041 gtatcagggc ctgggctggg aaatgctgga ctggccggta aatcctgaca gcatcattaa
    32101 cggcagtggc aataaaattg cactggcagc acaccccgta aaagcgatta cgcccccaac
    32161 tcctgcagta cgcgcatcat gggtacataa aacaggggca accggcggat ttggtagcta
    32221 tgtcgcgttt attccagaaa aagagctggg tatagtgatg ctggctaaca aaaactatcc
    32281 caatccagcg agagtcgccg ccgcctggca gattctcaac gctctacagt aaaatttcat
    32341 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
    32401 aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat
    32461 ttctggtcgt tgcctgcagt tctcctacgc cgccgcgtgg cgtaacagta gtgaataatt
    32521 tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg
    32581 aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg
    32641 tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt
    32701 actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg
    32761 gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg
    32821 accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg
    32881 agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc
    32941 agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca
    33001 gactcgccag gcgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg
    33061 cgccgactgc gccaataccc gtccacacgg cataagccgt ccctactggt aacgatttca
    33121 tcgcccaggc aagtagcgcc atactgacaa tcatcgccgt cacggtaata acactcggcg
    33181 tcaaacgact aaagccgtgg gtatatttca ggccaacggc ccataccact tccagcagac
    33241 cagcaataac taagataatc caggacatat caggctccgg agaattgggg ccgtccccgg
    33301 tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat attttgccag
    33361 gtggcagggg aatttcaact attaccttag tgactatcgc agaaaaaaca aacggcacga
    33421 catacctgtc gtaccgttat tgcttattgc tattgctgcg ctttcgttgc tgcgccagaa
    33481 atcgcgttac cgccatcaga aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt
    33541 aatactgttg aaagcaccag aacagaaaag atcgctgcaa ttgtcttctt caccataacg
    33601 tcttcctttt aggtaatgtt atttatgttt gcctatagca aattaataat agacaacatc
    33661 gccaaaaatg acggaaatct gatgattttt cggaagagaa gaaaaattag ctggcagcgc
    33721 gagagatgga gttgccgaga tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc
    33781 ccgtgagcag cgtgctggca agcaaaacaa gaacgataag gcgtttcatc atctctgacc
    33841 tgaataagtg atggtgcagc ctgcaggccg caccacaacc gcattacttc acgcgagaga
    33901 cgtattcacc agagcgggta tccactttga tgacttcgcc gatttgtacg aacagcggaa
    33961 ccttaaccac agcgccagta gacagggtag ccggtttgcc gccagtacct gcggtatcac
    34021 ctttcaggcc cggatcggta tcaacgattt ccagttcaac gaagttcggc ggagtaacgg
    34081 agatcggctg accattccac agagttacga tacactctgc ctgatccagc agccatttag
    34141 cgttgtcgcc aattgctttt gcatcagcag acagctgctc gaaagtttcg ttgttcatga
    34201 agtgccagaa ctcaccgtcg ttgtacaggt aagtcaggtt catatcgaca acatcagcgc
    34261 cttcagcgga atcagtagat ttgaaggttt tttctacgcg agtaccggtc agcagacgac
    34321 gcagtttaac gcgagcaaat gcctggcctt tacccggttt tacgaattca ctcgcttcaa
    34381 ccgcgtaagg ttcgccgtct aacatgattt taagaccagc acgaaaatcg ttgctatagt
    34441 acgttgccat aaggccctct gaaatttgtt aattggtagc taagccacaa aatggcgcat
    34501 attgtaaccc taaatacccc atccagagaa gattggttaa cgcaacttgc cgatgttgtg
    34561 accgatcctg atgaacttct gcgtcttttg aatatagacg cggacgaaaa actgttggcc
    34621 ggacgcagcg ccaaaaagct gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg
    34681 gagaaaggca atccggacga tcctcttttg cgtcaggtac ttacctcgca agatgagttt
    34741 gtcgtcgcgt ccggattctc caccgacccg ctggaagaac agcacagcgt agtgcctggt
    34801 ttgttgcata aataccacaa ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat
    34861 tgccgctatt gcttccgtcg ccacttcccc tatgccgaaa atcagggcaa caagcgtaac
    34921 tggcagactg ctcttgagta tgttgctgcg catccggaac tggacgagat gattttctcc
    34981 ggcggcgatc cgctgatggc gaaagatcac gagctggact ggttgctcac acaactggaa
    35041 gccatcccgc atatcaaacg tctgcggatt cacagccgtc tgccgattgt gatcccggca
    35101 cgtatcaccg aggcgctggt tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg
    35161 aatcacatca atcatgccaa tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg
    35221 cgccgtgtcg gtgtcaccct gcttaaccag agcgttctgt tacgtggtgt gaacgataac
    35281 gcacaaacgc tggcaaacct gagtaatgcg ttgttcgatg ccggcgtaat gccgtattac
    35341 ctgcatgtgc tcgataaagt acagggcgcg gcgcatttta tggtgagtga tgacgaagca
    35401 cggcagatta tgcgtgagtt gctgacactg gtgtcgggtt atctggtgcc gaaactggcg
    35461 cgagaaatcg gcggcgaacc cagcaaaacg ccgctggatc tccagctacg ccagcagtaa
    35521 ctgattaaac agggaagtta aacattagcc tgatgcgcta cgcttatcag gcctacattg
    35581 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
    35641 tccggcaaga gcaacaaacg caagtacagc aacctgtcat taaactggga aatttaatct
    35701 ttgcccgttg cataaccttt aattattcat atacattgct gttgttactc tttatatagc
    35761 caacaccaat aattaacctt tcttttctgt ttatatagag tgagtacggc tatttatgtt
    35821 attttaacaa ctattactac caagcccatt tatctggctt ataaattaaa attaattatt
    35881 taattgagaa atggttaggg agaacctaca tggccataag catcaagggt gttaataccg
    35941 gtgttattcg caagagtaat aatttcattg cactggcgct gaaaattaaa gaacctcgca
    36001 ataaagaatc gttgttcttt atgtctgtca tggaactgcg cgacctgctg attgcgctgg
    36061 aaagtcgtat gcatcaaaag cataaactgg atgctgctgc acgccttcag tatgagcaag
    36121 ctcgcgataa agtcattaag aaaatggcag aaaacatccc agaaattctg gttgatgagc
    36181 ttaaaaatgc cgatattaat cgtcgggtta acacgctgga acttaccgat aatcaaggcg
    36241 aaaatcttac cttcgtatta acgctacatg acggtagcaa atgtgagtta gtcgtcaatg
    36301 agttgcagat tgaaatgctg gcgcgagcaa tcattcatgc aattaataat gcggaaatgc
    36361 gtgaactggc attacgtatt acctcactgt tagatttcct gccgttatat gacgttgact
    36421 gccaggaaaa tggcaatctg gaatatgaca cctattccca accggaatgg aaacataacc
    36481 tgttcgacca ttatctggcg gtgctttacc gctttaaaga tgaaagtggc aaagaacaat
    36541 ttagcggcgc agtggtgaaa acgcgtgaag caacgccggg taaagaaata gaggcgatca
    36601 ctcgccgaat gcttgatttc agccaacgac tgaaaaaact cgcgggtgtg ccatgtcagg
    36661 tctatgttcg caccgtagcg gcgaataacg cccaaccgct aacccaggat caatgcttac
    36721 gcgcgctgca tcacttacgt gttcaatcca ccagcaaaac tgcaccacag gcgaaataat
    36781 ctcatctttg ctaatgcgag gctggagcca tccagcctga aatgactgat aacgtgatcg
    36841 ttggtcatgc cggatgcggc gtaaacgcct tatccggcct acaaattctc aagcattcaa
    36901 tcagttgcaa tgcctgctgt agatactgat aagcgaagcg catcaggcaa tgatacgttt
    36961 gtcacccagc ctctggatca catcagttcg ggcacttata aacctgaccg ataatctggc
    37021 tatccgtcgg gacaaaactg gacaacattc cctgcgacgg gctgctgatg ccataaatca
    37081 cgttaccgcc cattgcagcc gcctggttgc gcagatcgtt tgctgcgccg cgcatagaac
    37141 cgccctcttc tccgtgttgc ccggaaagcc agttgctttg cttacctgtc gcagtaccaa
    37201 tcagctggca ctctgcgcct ggctgctcgt ccacaatgcg tacactctga ccggcagcac
    37261 tcaattcgtt gctggagcta caacccgcca tcagtagcgc ggcaccgaca atccctgcta
    37321 agtattttac gtgcatgtta ttccccataa tcaatgagct ggacgccact cgcgtcgtcc
    37381 gtgtctgaat cttatactag aaagatgacc aaaagaaaaa cccccagaca tttctgcccg
    37441 ggggtttgtt tatttctgcg aggtgcaggg caattacatc atgccgccca tgccacccat
    37501 gccgcccata ccgccagcag cgcctaagtc agctgcatca tttttcggca ggtcggtaac
    37561 catgcactcg gtggtgatca tcaggccagc cacagaagcc gcgtactgca gagcagaacg
    37621 ggttactttg gttgggtcca ggatacccat gtcgatcatg ttgccgtatt cttcggttgc
    37681 tgcgttgtaa ccgtagttgc cgtcgccgcc tttaacggtg ttagcaacaa cagacggttc
    37741 ttcgccgcag ttcaggacga tctgacgcag cggagcttcc attgcacgca gtgcaacttt
    37801 gatacccacg ttctggtctt cgttctgacc acgcaggtca gccagtttag acgctacgcg
    37861 gatcagcgca acaccaccac cagcaaccac gccttcttct accgcagcac gggtcgcgtg
    37921 cagggcgtct tcaacgcgtg cttttttctc tttcatttca acttcggtag cagcacctac
    37981 tttgataact gcaacgccgc ctgccagttt cgctacgcgt tcctgcagtt tttcacggtc
    38041 gtagtcagaa gttgcttctt caatctgctg acggatctga gcaacacggc cctggattgc
    38101 agcttcttca cccacgccat cgatgatagt ggtggtgtct ttgttgatca caacacgttt
    38161 agcctgaccc aggtcttcca gggttgcttt ttccagctcc ataccgatct cttcagagat
    38221 tacggtaccg ccagtcaggg ttgcgatatc ctgcagcata gctttacgac gatcgccgaa
    38281 gcccggagct ttaaccgcag caactttcac gatgccacgc atggtgttaa caaccagagt
    38341 tgccagcgct tcgccttcaa catcttcagc gatgatcagc agcggtttgc ctgctttggc
    38401 aacagcttcc agaaccggca gcatttcgcg gatgttagag attttcttgt cagccagcag
    38461 gatgaacggg ctttccagtt ctactgcgcc agtttccggc ttgttgatga agtaaggaga
    38521 caggtagcca cggtcgaact gcataccttc aaccacgtcc agttcgtcct gcagaccggt
    38581 accgtcttca acggtgataa cgccttcttt accgactttg tccatcgctt cagcgatcag
    38641 tttacctacg gtttcgtcgg agttagcgga gatggtacca acctgagcaa tcgctttaga
    38701 gtcagagcac ggtacggaca gcgctttcag ttcttcaact gcagcggtaa cggctttgtc
    38761 gataccacgt ttcaggtcca tcgggttcat gcccgcagca acagctttca gaccctcagt
    38821 gatgatagcc tgagccagta cagttgcagt ggtggtaccg tcgcctgcag cgtcgttcgc
    38881 tttagaggca acttctttca ccatctgcgc acccatattt tcgaacttgt cttccagctc
    38941 gatttcacga gcaacggaaa caccatcttt ggtgatggtc ggtgcaccga aagatttatc
    39001 cagaactacg ttacggcctt tcggaccgag ggtaactttc actgcatctg ccagtacgtt
    39061 tacgccgcgc agcattttca cacgagcgtc gttaccgaat tttacgtctt tagctgccat
    39121 tatctttatt ccttaaattc gtatgttcag tgtcgtgcga ggattacgct tcaacaattg
    39181 ccagaatgtc gctttcggac atgatcaaca cttcttcatt gtcgatcttc tcagatttca
    39241 caccgtagcc atcgttgaaa ataacgatgt cgccaacttt cacatccagc ggcttcactt
    39301 cgccattttc aaggatacgg ccattgccga cagccagcac ttcgccgcgg gtggatttag
    39361 ccgctgcaga gccggtcaga acgatgccgc cagcagattt agtttcaact tctttacgct
    39421 tgacgatcac gcgatcatgc aatggacgaa tattcattga taactctcct ttgagaaagt
    39481 ccgtatctgt tatgggtgac gccggagctt acgtggtttc ccggctggtg accagagaaa
    39541 tggggatgag gattcgcccc ttcaaggggg aaaaagaaaa aaaattctga tcagcacaaa
    39601 atcgggtgaa aaccctgatt cacctcacat ttcatcgcaa tttcttcatc gccgtcataa
    39661 gcgaatctga ttgtgctacc atcgaaaatc tacgcagttg cccaaaattt gggcgcaatc
    39721 ggatatcaac ccatgagtgg actcaaacaa gaactggggc tggcccaagg cattggcctg
    39781 ctatcgacgt cattattagg cactggcgtg tttgccgttc ctgcgttagc tgcgctggta
    39841 gcgggcaata acagcctgtg ggcgtggccc gttttgatta tcttagtgtt cccgattgcg
    39901 attgtgtttg cgattctggg tcgccactat cccagcgcag gcggtgtcgc gcacttcgtc
    39961 ggtatggcgt ttggttcgcg acttgagcga gtcaccggct ggttgttttt atcggtcatt
    40021 cccgtgggtt tgcctgccgc gctacaaatt gccgccgggt tcggccaggc aatgtttggc
    40081 tggcatagct ggcaactgtt gttggcagaa ctcggtacgc tggcgctggt gtggtatatc
    40141 ggtactcgcg gtgccagttc cagtgctaat ctacaaaccg ttattgccgg acttatcgtc
    40201 gcgctgattg tcgctatctg gtgggcgggc gatatcaaac ctgcgaatat ccccttcccg
    40261 gcacctggta atatcgaact taccgggtta tttgctgcgt tatcagtgat gttctggtgt
    40321 tttgtcggtc tggaagcatt tgcccatctt gcctcggaat ttaaaaatcc agagcgtgat
    40381 tttcctcgcg cattgatgat tggtctgctg ctggcaggat tagtctactg gggctgtacg
    40441 gtagtcgtct tacacttcga cgcctatggt gaaaaaatgg cggcggcagc atcgcttcca
    40501 aaaattgtag tgcagttgtt cggtgtagga gcgttatgga ttgcctgcgt gattggctat
    40561 ctggcctgct ttgccagtct caacatttat atacagagct tcgcccgcct ggtctggtcg
    40621 caggcgcaac ataatcctga ccactacctg gcacgcctct cttctcgcca tatcccgaat
    40681 aatgccctca atgcggtgct cggctgctgt gtggtgagca cgttggtgat tcatgcttta
    40741 gagatcaatc tggacgctct tattatttat gccaatggca tctttattat gatttatctg
    40801 ttatgcatgc tggcaggctg taaattattg caaggacgtt atcgactact ggcggtggtt
    40861 ggcgggctgt tatgcgttct gttactggca atggtcggct ggaaaagtct ctatgcgctg
    40921 atcatgctgg cggggttatg gctgttgctg ccaaaacgaa aaacgccgga aaatggcata
    40981 accacataat ccggcgtttc gacattaatc ctggcgatcg tctttatgat caaggcggtc
    41041 gcgctcatca tcctttcgct ggtactcacc atcaaaagta ttaccgccac cggtcccggc
    41101 gctaaaaccg ccgcctggca tacgagaaaa gcgcaaatgc ggcatcaact tcactgtcag
    41161 atgcttttgc accggcggta ataaaagcag aagaccgagg aagtcggtga aaaagcccgg
    41221 taataaaagc agcaaaccgg caatgatcag cgaaacactt ttaatcatct ccgccgctgg
    41281 gttttcaccc gccgccattt tttgctgcat cagcacgaaa ttcttaaagc cctggttacg
    41341 taccagtgac atcccgataa ctgacgtgaa tataaccagc acgagggtca gcaatacccc
    41401 caatacatgg gcaacctgaa taaagattga aatctcaata tagacataaa ggaaaatggc
    41461 aataaaaggt aaccagcgca aaggtttctc ctgtaatagc agccggtaaa acccggctac
    41521 ctgaatgggt tgcgaatcgc gttcagctta tattgtggtc attagcaaaa tttcaagatg
    41581 tttgcgcaac tatttttggt agtaatccca aagcggtgat ctatttcaca aattaataat
    41641 taaggggtaa aaaccgacac ttaaagtgat ccagattacg gtagaaatcc tcaagcagca
    41701 tatgatctcg gatattcggt cgatgcaggg gataatcgtc ggtcgaaaaa cattcgaaac
    41761 cacatatatt ctgtgtgttt aaagcaaatc attggcagct tgaaaaagaa ggttcacatg
    41821 tcaaacaaca ttcgtatcga agaagatctg ttgggtacca gggaagttcc agctgatgcc
    41881 tactatggtg ttcacactct gagagcgatt gaaaacttct atatcagcaa caacaaaatc
    41941 agtgatattc ctgaatttgt tcgcggtatg gtaatggtta aaaaagccgc agctatggca
    42001 aacaaagagc tgcaaaccat tcctaaaagt gtagcgaatg ccatcattgc cgcatgtgat
    42061 gaagtcctga acaacggaaa atgcatggat cagttcccgg tagacgtcta ccagggcggc
    42121 gcaggtactt ccgtaaacat gaacaccaac gaagtgctgg ccaatatcgg tctggaactg
    42181 atgggtcacc agaaaggtga atatcagtac ctgaacccga acgaccatgt taacaaatgt
    42241 cagtccacta acgacgccta cccgaccggt ttccgtatcg cagtttactc ttccctgatt
    42301 aagctggtag atgcgattaa ccaactgcgt gaaggctttg aacgtaaagc tgtcgaattc
    42361 caggacatcc tgaaaatggg tcgtacccag ctgcaggacg cagtaccgat gaccctcggt
    42421 caggaattcc gcgctttcag catcctgctg aaagaagaag tgaaaaatat ccaacgtacc
    42481 gctgaactgc tgctggaagt taaccttggc gcaacagcaa tcggtactgg tctgaacacg
    42541 ccgaaagagt actctccgct ggcagtgaaa aaactggctg aagtcactgg cttcccatgc
    42601 gtaccggctg aagacctgat cgaagcgacc tctgactgcg gcgcttatgt tatggttcac
    42661 ggcgcgctga aacgcctggc tgtgaagatg tccaaaatct gtaacgacct gcgcttgctc
    42721 tcttctggtc cacgtgccgg cctgaacgag atcaacctgc cggaactgca ggcgggctct
    42781 tccatcatgc cagctaaagt aaacccggtt gttccggaag tggttaacca ggtatgcttc
    42841 aaagtcatcg gtaacgacac cactgttacc atggcagcag aagcaggtca gctgcagttg
    42901 aacgttatgg agccggtcat tggccaggcc atgttcgaat ccgttcacat tctgaccaac
    42961 gcttgctaca acctgctgga aaaatgcatt aacggcatca ctgctaacaa agaagtgtgc
    43021 gaaggttacg tttacaactc tatcggtatc gttacttacc tgaatccgtt catcggtcac
    43081 cacaacggtg acatcgtggg taaaatctgt gccgaaaccg gtaagagtgt acgtgaagtc
    43141 gttctggaac gcggtctgtt gactgaagcg gaacttgacg atattttctc cgtacagaat
    43201 ctgatgcacc cggcttacaa agcaaaacgc tatactgatg aaagcgaaca gtaatcgtac
    43261 agggtagtac aaataaagaa ggcacgtcag atgacgtgcc ttttttcttg tgagcagtaa
    43321 cttaaaaata acaacctaat atcaacttgt taaaaaacaa ggaaggctaa tatgctagtt
    43381 gtagaactca tcatagtttt gctggcgatc ttcttgggcg ccagattggg gggaataggt
    43441 attggttttg caggcggatt gggggtgctg gttcttgccg ctattggcgt taaacccggt
    43501 aacatcccgt tcgatgtcat ttccattatc atggcggtta tcgccgctat ttctgccatg
    43561 caggttgctg gcggtctgga ctatctggtt catcagacag aaaagctgct gcgccgtaac
    43621 ccgaaataca tcacgatcct cgcaccgatc gtgacctatt tcctgactat ctttgctggt
    43681 actggcaaca tatctctggc gacactgcca gttatcgctg aagttgcgaa ggaacaaggc
    43741 gtcaaacctt gccgtccgct gtctactgca gtggtatccg cgcagattgc gatcaccgca
    43801 tcgccaatct cagcggcagt ggtttacatg tcttccgtga tggaaggtca tggcatcagc
    43861 tacctccatc tgctctccgt ggtcatcccg tccaccctgc tggcggttct ggtgatgtcc
    43921 tttctggtca ctatgctgtt caactccaaa ctctctgacg atccgattta tcgcaagcgt
    43981 ctggaagagg gcctggttga actgcgcggt gaaaagcaga ttgaaatcaa atccggtgca
    44041 aaaacgtccg tctggctgtt cctgctgggc gtagttggcg tggttatcta tgcaatcatc
    44101 aacagcccaa gcatgggtct ggttgaaaaa ccgctgatga acaccaccaa cgcaatcctg
    44161 atcatcatgc tcagcgttgc aactctgacc accgttatct gtaaagtcga taccgacaac
    44221 atcctcaact ccagcacctt caaagcaggt atgagcgcct gtatttgtat cctgggtgtt
    44281 gcgtggctgg gcgatacttt cgtttccaac aacatcgact ggatcaaaga taccgctggt
    44341 gaagtgattc agggtcatcc gtggctgctg gccgtcatct tcttctttgc ttctgctctg
    44401 ctgtactctc aggctgcaac cgcaaaagca ctgatgccga tggccctggc actgaacgtt
    44461 tcaccgctga ccgctgttgc ttctttcgct gcggtgtctg gtctgttcat tctgccgacc
    44521 tacccgacgc tggttgctgc ggtacagatg gatgacacgg gtacaacccg tatcggtaaa
    44581 ttcgtcttca accatccgtt cttcatcccg ggtactctgg gtgttgccct ggccgtttgc
    44641 ttcggcttcg tgctgggtag cttcatgctg taatgaccaa tcgcggggcg ttcacgcccc
    44701 gctttctttc ccgccgacta acatccttcc cccgtccgtt gtatagtgac ctctctcttg
    44761 cggttccatc tgttcttgcg aggtgtttat gcttgatgaa aaaagttcga ataccgcgtc
    44821 tgtcgtggtg ctatgtaccg caccggatga agcgacagcc caggatttag ccgccaaagt
    44881 gctggcggaa aaactggcgg cctgcgcgac cttgatcccc ggcgctacct ctctctatta
    44941 ctgggaaggt aagctggagc aagaatacga agtgcagatg attttaaaaa ctaccgtatc
    45001 tcaccagcag gcactgctgg aatgcctgaa gtctcatcat ccatatcaaa ccccggaact
    45061 tctggtttta cctgttacac acggagacac agattacctc tcatggctca acgcatcttt
    45121 acgctgatcc tgctactttg cagcacttcc gtttttgccg gattattcga cgcgccggga
    45181 cgttcacatt ttgtccccgc ggatcaagcc tttgcttttg attttcagca aaaccaacat
    45241 gacctgaatc tgacctggca gatcaaagac ggttactacc tctaccgtaa acagatccgc
    45301 attacgccgg aacacgcgaa aattgccgac gtgcagctgc cgcaaggcgt ctggcatgaa
    45361 gatgagtttt acggcaaaag cgagatttac cgcgatcggc tgacgcttcc cgtaaccatc
    45421 aaccaggcga gtgcgggagc gacgttaact gtcacctacc aaggctgtgc tgatgccggt
    45481 ttctgttatc cgccagaaac caaaaccgtt ccgttaagcg aagtggtagc caacaacgca
    45541 gcgtcacagc ctgtgtctgt tccgcagcaa gagcagcaca ccgcgcaatt gcccttttcc
    45601 gcgctctggg cgttgttgat cggtattggt atcgccttta cgccatgcgt gctgccaatg
    45661 tacccactga tttctggcat cgtgctgggt ggtaaacagc ggctctccac tgccagagca
    45721 ttgttgctga cctttattta tgtgcagggg atggcactga cttacacggc gctgggtctg
    45781 gtagttgccg ccgcagggtt acagttccag gcggcgctac agcacccata cgtgctcatt
    45841 ggcctcgcca tcgtctttac cttgctggcg atgtcaatgt ttggcttgtt tactctacaa
    45901 ctcccctctt cgctgcaaac acgtctcacg ttgatgagca atcgccaaca gggcggctca
    45961 cctggcggtg tgtttgttat gggggcgatt gccggactga tctgttcacc atgcaccacc
    46021 gcaccgctta gcgcgattct gctgtatatc gcccaaagcg ggaacatgtg gctgggcggc
    46081 ggcacgcttt atctctatgc gttgggcatg ggcctgccgc tgatgctaat taccgtcttt
    46141 ggtaaccgct tgctgccgaa aagcggcccg tggatggaac aagtcaaaac cgcgtttggt
    46201 tttgtgatcc tcgcactgcc ggtcttcctg ctggagcgag tgattggtga tgtatgggga
    46261 ttacgcttgt ggtcggcgct gggtgtcgca ttctttggct gggcctttat caccagccta
    46321 caggctaaac gcggctggat gcgcgtggtg caaataatcc tgctggcagc ggcattggtt
    46381 agcgtgcgcc cacttcagga ttgggcattt ggtgcgacgc ataccgcgca aactcagacg
    46441 catctcaact ttacacaaat caaaacggta gatgagttaa atcaggcgct cgttgaagtc
    46501 aaaggcaaac cggtgatgtt agatctttat gccgactggt gcgtcgcctg taaagagttt
    46561 gagaaataca ccttcagcga cccgcaggtg caaaaagcgt tagcagacac ggtcttactt
    46621 caggccaacg tcacggccaa cgacgcacaa gatgtggcgc tgttaaagca tcttaatgtc
    46681 cttggcctac cgacaattct cttttttgac ggacaaggcc aggagcatcc acaagcacgc
    46741 gtcacgggct ttatggatgc tgaaaccttc agcgcacatt tgcgcgatcg ccaaccgtga
    46801 acgacacttg cagtgggaaa gacggaggaa aatagcgtgc aacgtgaaga tgtactggga
    46861 gaagccctga aattattaga attacaaggg attgccaaca ccacgctgga gatggttgct
    46921 gaacgtgtgg attatccact ggacgagcta cgccgcttct ggccagacaa agaggcaatc
    46981 ctctacgatg cgctgcgtta tcttagccaa cagatagatg tctggcgtcg tcagctgatg
    47041 ctggacgaaa cgcaaaccgc cgaacaaaag ctgctggcac gttatcaggc gttatcggag
    47101 tgcgttaaaa acaaccgcta tccgggctgt ctatttatcg ctgcctgtac gttttatccc
    47161 gatcctggcc accctattca tcaactggcc gatcagcaaa aaagcgcggc ctacgatttc
    47221 acccacgaac tgttaaccac gctggaagtt gacgatccgg cgatggtagc aaagcagatg
    47281 gaactggtgc tggaaggctg tttaagccga atgctggtga atcgtagtca ggcggatgtc
    47341 gacactgcac atcggctggc ggaagatatc ctgcgcttcg cccgctgccg ccagggtggt
    47401 gcattgacct gacagaaaca cagaaaagaa gcgatttgcc gcaatcttaa gcagttgaat
    47461 cgcttttact gaaattaggt tgacgagatg tgcagattac ggtttaatgc gccccgttgc
    47521 ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg gttcgattcc
    47581 gagtccgggc accaaattca tatcaacgga cctccacgga ggtccgtttt tcgtttcaga
    47641 acaccatgat ttaagtgttc ttcctccaaa tcaattctac cgaactcaac caaattctcc
    47701 ccacatcaac ctcattgtgt gggtataatt gcgggcatac cccagttcga cagaatttgt
    47761 accctcttta acgcattcag aaggctgaac tatggcactg actgacgcaa aaatccgggc
    47821 tgcaaagccc actgacaagg cttataaact cactgacggg gctggcatgt tcctgctggt
    47881 acatcctaat ggttcccgtt actggcgtct ccgttatcgt attctgggta aagagaagac
    47941 tctggcactt ggtgtgtatc cagaagtatc tctctccgaa gctcgtacaa aacgggatga
    48001 ggcccgaaaa ctgatttcgg aggggggtga cccttgcgaa cagaaaagag ctaaaaaagt
    48061 agtccctgat ttacaactct ctttcgaaca tattgcacga cgctggcatg ccagtaataa
    48121 acaatgggca caatcacaca gcgataaagt actcaaaagc ctcgagacac acgtattccc
    48181 ctttatcggc aatcgggata tcacaacact caataccccg gacctgctta tccctgttcg
    48241 tgctgcagaa gctaaacaaa tttatgaaat cgccagtcgt ctgcagcaaa gaatatctgc
    48301 cgtaatgcgt tatgccgtac agtctggcat catcagatat aatcctgctc tggatatggc
    48361 tggcgcattg actacggtaa aacgccagca tcgccccgct cttgatcttt ctcgcctgcc
    48421 tgaacttttg tcgcgtattg gcagttacaa ggggcaacct gtcacccagc ttgccgttat
    48481 gctgaattta ctggttttta ttcgttccag tgaactcaga tacgcccgct ggtctgaaat
    48541 tgatattgac aatgccatgt ggactattcc agccgaacgc gaacctctgc caggcgtaaa
    48601 attctcacac cggggatcca agatgcgaac accacatctt gtgccactca gcaaacaggt
    48661 tgtagccata ctggcagaac ttcagacatg ggctggtgaa aatggtctga tctttacggg
    48721 agcacatgac ccacgtaaac caatcagtga aaacaccgta aataaagccc tgagggttat
    48781 ggggtatgac acaacccagg atgtctgtgg tcatggattc cgggcgatgg catgtagtgc
    48841 gttaatagaa tcaggtttgt ggtcccgcga tgcagttgaa cgtcagatga gccatcagga
    48901 acgtaacggt gtacgtgctg cttacattca taaagcagaa catctggaag aacggcgact
    48961 gatgctacag tggtgggccg attttctgga tgcaaacaga gaaagattta tcagtccatt
    49021 tgaatatgca aagattaata atccattaaa acagtaatca tcccgggcaa atgcccggga
    49081 acctactcca ggattatttt ctttgttaaa aaaacagaca aaacggtatt aac
//
LOCUS       DAZGTR010000020        46305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGTR010000020 DAZGTR010000000
VERSION     DAZGTR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.020"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHLCY_001332"
     CDS             complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHLCY_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP0368628.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2090..2956
                     /gene="yghU"
                     /locus_tag="ACHLCY_001333"
     CDS             2090..2956
                     /gene="yghU"
                     /locus_tag="ACHLCY_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP0368629.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3080..3328)
                     /gene="hybG"
                     /locus_tag="ACHLCY_001334"
     CDS             complement(3080..3328)
                     /gene="hybG"
                     /locus_tag="ACHLCY_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP0368630.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3341..3682)
                     /gene="hypA"
                     /locus_tag="ACHLCY_001335"
     CDS             complement(3341..3682)
                     /gene="hypA"
                     /locus_tag="ACHLCY_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0368631.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3675..4163)
                     /gene="hybE"
                     /locus_tag="ACHLCY_001336"
     CDS             complement(3675..4163)
                     /gene="hybE"
                     /locus_tag="ACHLCY_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP0368632.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4156..4650)
                     /locus_tag="ACHLCY_001337"
     CDS             complement(4156..4650)
                     /locus_tag="ACHLCY_001337"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP0368633.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4650..6353)
                     /gene="hybC"
                     /locus_tag="ACHLCY_001338"
     CDS             complement(4650..6353)
                     /gene="hybC"
                     /locus_tag="ACHLCY_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP0368634.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6350..7528)
                     /gene="hybB"
                     /locus_tag="ACHLCY_001339"
     CDS             complement(6350..7528)
                     /gene="hybB"
                     /locus_tag="ACHLCY_001339"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP0368635.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7518..8504)
                     /gene="hybA"
                     /locus_tag="ACHLCY_001340"
     CDS             complement(7518..8504)
                     /gene="hybA"
                     /locus_tag="ACHLCY_001340"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP0368636.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8507..9625)
                     /gene="hybO"
                     /locus_tag="ACHLCY_001341"
     CDS             complement(8507..9625)
                     /gene="hybO"
                     /locus_tag="ACHLCY_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP0368637.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9814..10101)
                     /gene="yghW"
                     /locus_tag="ACHLCY_001342"
     CDS             complement(9814..10101)
                     /gene="yghW"
                     /locus_tag="ACHLCY_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP0368638.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10220..11107)
                     /gene="yghX"
                     /locus_tag="ACHLCY_001343"
     CDS             complement(10220..11107)
                     /gene="yghX"
                     /locus_tag="ACHLCY_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP0368639.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11264..12304
                     /gene="gpr"
                     /locus_tag="ACHLCY_001344"
     CDS             11264..12304
                     /gene="gpr"
                     /locus_tag="ACHLCY_001344"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP0368640.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12344..12838)
                     /locus_tag="ACHLCY_001345"
     CDS             complement(12344..12838)
                     /locus_tag="ACHLCY_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP0368641.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13029..13913
                     /gene="yghA"
                     /locus_tag="ACHLCY_001346"
     CDS             13029..13913
                     /gene="yghA"
                     /locus_tag="ACHLCY_001346"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0368642.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14185..14610)
                     /gene="exbD"
                     /locus_tag="ACHLCY_001347"
     CDS             complement(14185..14610)
                     /gene="exbD"
                     /locus_tag="ACHLCY_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP0368643.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14617..15351)
                     /gene="exbB"
                     /locus_tag="ACHLCY_001348"
     CDS             complement(14617..15351)
                     /gene="exbB"
                     /locus_tag="ACHLCY_001348"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0368644.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15603..16790
                     /gene="metC"
                     /locus_tag="ACHLCY_001349"
     CDS             15603..16790
                     /gene="metC"
                     /locus_tag="ACHLCY_001349"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP0368645.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16930..17589
                     /gene="yghB"
                     /locus_tag="ACHLCY_001350"
     CDS             16930..17589
                     /gene="yghB"
                     /locus_tag="ACHLCY_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP0368646.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17629..18528)
                     /gene="yqhC"
                     /locus_tag="ACHLCY_001351"
     CDS             complement(17629..18528)
                     /gene="yqhC"
                     /locus_tag="ACHLCY_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP0368647.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18722..19885
                     /gene="yqhD"
                     /locus_tag="ACHLCY_001352"
     CDS             18722..19885
                     /gene="yqhD"
                     /locus_tag="ACHLCY_001352"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP0368648.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19990..20817
                     /gene="dkgA"
                     /locus_tag="ACHLCY_001353"
     CDS             19990..20817
                     /gene="dkgA"
                     /locus_tag="ACHLCY_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP0368649.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            21017..21943
                     /locus_tag="ACHLCY_001354"
     CDS             21017..21943
                     /locus_tag="ACHLCY_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP0368650.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21994..22251
                     /gene="yqhH"
                     /locus_tag="ACHLCY_001355"
     CDS             21994..22251
                     /gene="yqhH"
                     /locus_tag="ACHLCY_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP0368651.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22294..24513)
                     /locus_tag="ACHLCY_001356"
     CDS             complement(22294..24513)
                     /locus_tag="ACHLCY_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP0368652.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALAGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24624..26036)
                     /gene="ftsP"
                     /locus_tag="ACHLCY_001357"
     CDS             complement(24624..26036)
                     /gene="ftsP"
                     /locus_tag="ACHLCY_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP0368653.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26111..26848)
                     /gene="plsC"
                     /locus_tag="ACHLCY_001358"
                     /gene_synonym="parF"
     CDS             complement(26111..26848)
                     /gene="plsC"
                     /locus_tag="ACHLCY_001358"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP0368654.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27081..29339)
                     /gene="parC"
                     /locus_tag="ACHLCY_001359"
     CDS             complement(27081..29339)
                     /gene="parC"
                     /locus_tag="ACHLCY_001359"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP0368655.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(29885..30367)
                     /locus_tag="ACHLCY_001360"
     CDS             complement(29885..30367)
                     /locus_tag="ACHLCY_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP0368656.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPIMFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30420..30812)
                     /gene="ygiW"
                     /locus_tag="ACHLCY_001361"
     CDS             complement(30420..30812)
                     /gene="ygiW"
                     /locus_tag="ACHLCY_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP0368657.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30964..31623
                     /gene="qseB"
                     /locus_tag="ACHLCY_001362"
     CDS             30964..31623
                     /gene="qseB"
                     /locus_tag="ACHLCY_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP0368658.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31620..32969
                     /gene="qseC"
                     /locus_tag="ACHLCY_001363"
     CDS             31620..32969
                     /gene="qseC"
                     /locus_tag="ACHLCY_001363"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP0368659.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(33015..33347)
                     /locus_tag="ACHLCY_001364"
     CDS             complement(33015..33347)
                     /locus_tag="ACHLCY_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFP0368660.1"
                     /translation="MLKHKIQTIFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLF
                     WGRFIFCY"
     gene            33666..34247
                     /gene="mdaB"
                     /locus_tag="ACHLCY_001365"
     CDS             33666..34247
                     /gene="mdaB"
                     /locus_tag="ACHLCY_001365"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP0368661.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            34278..34592
                     /locus_tag="ACHLCY_001366"
     CDS             34278..34592
                     /locus_tag="ACHLCY_001366"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP0368662.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34640..36532)
                     /gene="parE"
                     /locus_tag="ACHLCY_001367"
     CDS             complement(34640..36532)
                     /gene="parE"
                     /locus_tag="ACHLCY_001367"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP0368663.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(36561..37142)
                     /gene="yqiA"
                     /locus_tag="ACHLCY_001368"
     CDS             complement(36561..37142)
                     /gene="yqiA"
                     /locus_tag="ACHLCY_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP0368664.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNSAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(37142..37969)
                     /gene="cpdA"
                     /locus_tag="ACHLCY_001369"
     CDS             complement(37142..37969)
                     /gene="cpdA"
                     /locus_tag="ACHLCY_001369"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP0368665.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYRHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37994..38416)
                     /locus_tag="ACHLCY_001370"
     CDS             complement(37994..38416)
                     /locus_tag="ACHLCY_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP0368666.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(38417..39046)
                     /gene="nudF"
                     /locus_tag="ACHLCY_001371"
     CDS             complement(38417..39046)
                     /gene="nudF"
                     /locus_tag="ACHLCY_001371"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP0368667.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            39251..40732
                     /gene="tolC"
                     /locus_tag="ACHLCY_001372"
     CDS             39251..40732
                     /gene="tolC"
                     /locus_tag="ACHLCY_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP0368668.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40880..41551
                     /locus_tag="ACHLCY_001373"
     CDS             40880..41551
                     /locus_tag="ACHLCY_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP0368669.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            41557..42717
                     /locus_tag="ACHLCY_001374"
     CDS             41557..42717
                     /locus_tag="ACHLCY_001374"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0368670.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(42755..43543)
                     /gene="ygiD"
                     /locus_tag="ACHLCY_001375"
     CDS             complement(42755..43543)
                     /gene="ygiD"
                     /locus_tag="ACHLCY_001375"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP0368671.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43686..44459
                     /gene="zupT"
                     /locus_tag="ACHLCY_001376"
     CDS             43686..44459
                     /gene="zupT"
                     /locus_tag="ACHLCY_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP0368672.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(44517..44687)
                     /gene="yqiD"
                     /locus_tag="ACHLCY_001377"
     CDS             complement(44517..44687)
                     /gene="yqiD"
                     /locus_tag="ACHLCY_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP0368673.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44948..45601)
                     /gene="ribB"
                     /locus_tag="ACHLCY_001378"
     CDS             complement(44948..45601)
                     /gene="ribB"
                     /locus_tag="ACHLCY_001378"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP0368674.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45705..45853)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45975..46265
                     /gene="ubiK"
                     /locus_tag="ACHLCY_001379"
     CDS             45975..46265
                     /gene="ubiK"
                     /locus_tag="ACHLCY_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP0368675.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11486 a        12374 c        11580 g        10865 t
ORIGIN      
        1 cagtacgggg ttgctacaac atgtattact ttttcaccac aattaacggt tcaatatcac
       61 tctctttttt gatgaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc
      121 caacggtgaa ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt
      181 gctgatagat gtttttctgc tcggcaaatt taccgctggt tttgtccagc acctcttcat
      241 gatggctgac caggtcgata ttgctaccac agcgaccggc aatcggtttc actgcgtaac
      301 ctgttttcac caattcatca ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg
      361 ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg
      421 gctcaaagac tagcacttcc gggcgcagca atacgtcgat aagacgcact tcgttttgcg
      481 gatgaccggt acggattggc accgcagcaa actcacggtc gctaacttca cgaatctgat
      541 caaacgcggt ttcccacgcc caggttttcc acacgcagtt aaccagccgt ccttccccat
      601 caatcagttg cccggcagca tcccagccca gttcatccag cccgcgcaag atacgcgttt
      661 caaagcccgc ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat
      721 ctttgtcctg catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca
      781 gttcgttaat cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc
      841 aacgttcgag gatcaagccc gcttcggtat gacatgaggc agagtcggcg ttgtattcgt
      901 aaaccttcag gccacgctca tccatgcaga aatccatacg accagtgatc atatggtgac
      961 ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat tttcgggatg tcgaacagcg
     1021 ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg aagatacatc aggtgcagct
     1081 cgttggtggc tttaattagc tcctgctcgg cactctcggt aatggtgtag taatgataag
     1141 gatcctgatt gatcacctga ccgttggcct gcacataggc gttttgcagc ggatcttttt
     1201 catccagcca tttaccgtca aactggcctt tgttttccag gcgcgctccg ctgattttca
     1261 gcagctcgcc tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca
     1321 tccagcccag aatggtggtg tcatcaaaag tgtctttcag ggtatagcag ccgttttcga
     1381 ccaccatctc cagctcacgc gtccactgtt gcccttgcgg caacggggaa tgaatcacgt
     1441 tctgttccgc aatacggact ttgttgccat gcaattgggt aatgatggcg acatggccag
     1501 tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg
     1561 gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc attaaccact tctcgcagga
     1621 agcgcagcga gaaaatctcc cacgccatac ccacgtcagt aaagaccaca ccgtaattca
     1681 gaaagagaaa acggcgagca aattcaacgc attgccactt gtggcccata tattcatcgt
     1741 cgatatagct acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat
     1801 ctgaagagta gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat
     1861 cctggctggt cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg
     1921 cgtaattgtt gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa
     1981 tcatacacct catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca
     2041 agtgacctgc tacactgctt caacactacc gctcagaagg caactcacta tgacagacaa
     2101 tacttatcag cccgcgaaag tctggacgtg ggataaatcc gctggcggcg cgttcgccaa
     2161 tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg ctgcccgttg gcaaacaccc
     2221 attgcaactt tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga
     2281 gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc tggctgattc gtattggcga
     2341 tggcgatcaa ttctccagcg gctttgtcga agtgaaccca aactcgaaga tcccggcgct
     2401 gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa tctggttcga tcctgcttta
     2461 tctggcggag aaatttggct acttcctgcc gcaggatttg gcaaagcgta ctgaaacgat
     2521 gaactggctg ttctggttac agggcgcggc accgttcctc ggcggtggtt ttggtcactt
     2581 ttaccattac gcgccgatga aaattgagta cgccatcaac cgctttacca tggaagccaa
     2641 acgtctgctc gacgtgctgg ataagcaact ggcgcagcat aagtttattg cgggcgatga
     2701 gtacaccatt gcggatatgg cgatttggcc gtggtttggc aatgtggtgt taggcggcgt
     2761 gtatgatgcc gcagagtttc ttgatgcggg cagttataag catgtacaac gctgggcgaa
     2821 agaagtaggc gaacgtccgg cggtgaaacg tgggcgtatt gttaaccgca ccaacggacc
     2881 gctgaatgag cagttgcatg agcgccatga cgccagtgat ttcgagacga atacggaaga
     2941 taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc catccaagcc ggataaggcg
     3001 ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg acgctgccgc gtcttatcag
     3061 tatctacgaa agctaatcat cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt
     3121 ctaatgtggc tttggcttca tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca
     3181 gcacccactg gcccagtaga tcggcagggt taccttcaca aatcagggcg atattcacgt
     3241 cgcgcttgat accacatact tcaacctgcg caagctggtg aatatcttca ccgacagcca
     3301 gcacctggcc tggaacgcca atacacatta ttaactccgg ttattcaact tcaatacttt
     3361 tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact
     3421 gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc ccagcaccaa gcctgggcgg
     3481 gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa
     3541 aactaaaacg gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg
     3601 cggtgacgcg cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct
     3661 ggcaaagaga caactcatgc attttcgcca ctcctgcgac caaacagcag ggcgcgacgc
     3721 cctgcgtgtg gtacatccgg attcgtgact ggcagcgaaa ggatcattcg tgcgcagtca
     3781 tccgtcaggc gttggccctc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa
     3841 caggagagat attgcgaaac accgtccagt tcgccaacgg taaaggtcat agtgccatac
     3901 ggcagttgca gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg
     3961 gggaagataa ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg
     4021 aacagcgtaa aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc
     4081 atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg
     4141 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgccagattc
     4201 acgcagcgca gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt
     4261 taagccgatg tgtggctcca gcgattccgg gatcacaccg accagggtca gctttttcgg
     4321 aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga
     4381 gattttgttg gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg
     4441 cgtgttcttt ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
     4501 gccaagcagc tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat
     4561 gtatcgttgc tctaacgctt cgacaatccg cacaccgatg gcttcatcgg tcagcaaaat
     4621 attgccgacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
     4681 ccgtcggcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
     4741 cgcaccactt ccagcggttt attcggatca gcaaccggtg tacccaccag cgactgctcg
     4801 taaggaccga cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaact
     4861 gcctggtagt tgctgatgat accgtcttta atcaccatcc agtgagagag cataccgcgc
     4921 ggcgcttcga ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggtttcaca
     4981 aaggtggtgt gatcgccttt gccgatattg gtgatcagtg cactgtattg gttttgcagg
     5041 atatcctgca attcacagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc
     5101 agctgtgcca cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc
     5161 agtttgttct gggtagattc tcgacctgcc gccagtttca ccagcatatt agccagcgga
     5221 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg
     5281 tcagaccaac catcataagc cggaatggtg gtaccttccc acggcgcctg tggcgcttcg
     5341 tctttatacc aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa
     5401 tgagaagtga tcggacgata cgaggacaga tccgcattct caatgtagcc gcccgggaac
     5461 aggaagctgc cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca
     5521 cctttaccgc gcgtcagcca ttccgggtag aacgcggcga taaccgcggt atcaacctta
     5581 taaacctgct caacaaagtc gctcagtttg tcgatgaaag acttgatgta catcaggcgc
     5641 tcaaggttca acacgcccaa cccgtcgagg ttgattgggt tcgcgacacc acccactgcc
     5701 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
     5761 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
     5821 ttcatcgctg ggtgacccca gtagccatta gcgaaaatac ccaactgacc gctggcaacc
     5881 agatctttga tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc
     5941 gaaacgcctt tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg
     6001 tccacccagt ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc
     6061 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca
     6121 ctttctgccg cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt
     6181 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
     6241 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
     6301 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
     6361 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
     6421 tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
     6481 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccagc gaataggtca
     6541 gacgccaggt tgcacaacct aacagtgcgc tcagtgctga caggaacagc atgcgggaat
     6601 cattacgcag cttcgccaca cgcagaacca ccagcgggaa gagcatcagc aggacttcaa
     6661 tccagaacat cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
     6721 taagctcgcc aaagcgcagc acgatgaaaa tcgccagcaa cacactgatg gtgttggtca
     6781 gcttgacgaa cagactcttt tcatccggac cgttgccacg cagacccgcc tgcaccagcg
     6841 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
     6901 gcaacatttc atagctctgc cacaacggat gcaccttgta gcccgccgag atcatcagtg
     6961 accccattga agactggtgc atggtcggca acagcgcgcc aagtgcaatg atgaagaaca
     7021 tcaccttgtt cagacgcttg agagaaacct tccagcccag acgttcaaac agtgccggag
     7081 caaactccag tgccatcacg ccgatataga tggtcataca gaccgccgtc tcgaacagta
     7141 ccgagttcac gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccca
     7201 cgtcgatagt gatcgacaag ccccccagtg agtaaccaaa cagactcgcc aacagcgccg
     7261 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
     7321 agccgccaca ggcaaagccg gtaccaatca gcaggtcaaa cgcgatccac acgccccacg
     7381 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
     7441 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
     7501 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
     7561 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
     7621 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
     7681 ggcaggtcga gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc
     7741 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
     7801 gatttcagcg tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt
     7861 ttcgcctccg ccatcagctc ttcacgcgta ccaaaaatta ccgcgcccgc cgggcacact
     7921 tctacgcagc caggcaaacc gcctttatcg agacgttcca cacctttctg gttgcacaac
     7981 tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtatggg
     8041 caggcgacca tgcagtaacg gcaaccggtg cacacatctt tgtcgtaatg gacaatgccg
     8101 gttttcggat cttttttcag cgcagagacc gggcacacag agacacagtt cggatcgacg
     8161 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
     8221 cctgtgccgc tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg
     8281 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg
     8341 gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc
     8401 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
     8461 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
     8521 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
     8581 cggcaaccag cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc
     8641 cctctttagc gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
     8701 gctgatggat gcctttatgg aagccgatac cttcttcgtt acagccatag catgggtgac
     8761 caatcgctac cggccacacg ccgccaacat cgcagaattg cagcgttgag cagttgccgt
     8821 aagtttctgg ccctttacag ccgaggtggt acaggcacca gccttcacgg tggccttcat
     8881 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
     8941 gaatcagacg gccataggcg aaggtcggac gatttttgtc atccagtttc ggcggtttgc
     9001 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
     9061 gaatattgat aacggttttg cctggcagaa cttcttgcag gctgactgcg ccagttgggt
     9121 taactccagc tgcggcaaca ccgccccacg cagagcagga accgatagcg ataatcgctg
     9181 ctgcgccttc tgccgctttg cggatgtgat ccacaatcgg ctcaccagca accatgcaat
     9241 aaataccgtt atcttttaat gggatggaac catccacaac caacacatac tgccctttgt
     9301 acttctcaag tgcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca
     9361 cttcgtgata ctccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
     9421 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
     9481 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
     9541 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
     9601 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgaagacc tggcatatat
     9661 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
     9721 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
     9781 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat
     9841 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
     9901 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
     9961 ggtcttttcg tacatccggt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
    10021 gaattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
    10081 tttaccaaaa tggttattca tcgttcacct cattcgctat atatcgccat atatagcgat
    10141 attttaggca aagaacgact tttgcgcacg ctattttttc atgcttgttt aagacgtaat
    10201 ccagtaagag ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc
    10261 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
    10321 taacccccgg atagatatac gcctcataaa ccttattatt ggctttcaac gccgcctcgt
    10381 aagcaggcca gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg
    10441 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
    10501 ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg
    10561 taatacccac tttgcctgcc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga
    10621 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
    10681 ttcccggata acctccaacg gaacttaagc cgtcaggtgc cagggcgata taccccgcct
    10741 tcgccactcg ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca
    10801 ctaccacggc tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct
    10861 cgccgtgacc atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa
    10921 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg
    10981 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gagattttcc
    11041 cgtgagcgta atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac
    11101 gcggcatcgt tgcactcctc aactggtgtt tttttaagca aagcataagc acgtgttttt
    11161 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
    11221 ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa
    11281 tcccgaacgt tacgggcaga tgcaataccg ctattgcgga aaaagcggtt tacgcctgcc
    11341 cgcgttatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
    11401 tgcgatcctg cgtaaagcgt ttgatttggg cattacgcac tttgatttag ccaacaatta
    11461 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcggg aggattttgc
    11521 cgcttatcgc gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc
    11581 ttacggctct ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
    11641 tatggggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
    11701 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
    11761 gatctcctct tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa
    11821 aattccgctt ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
    11881 cggcctgctg gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc
    11941 tcagggattg ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgtatcg
    12001 tgaagggaat aaagttcgtg gtctgacacc gaaaatgctt accgaagcca acctcaacag
    12061 cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
    12121 aagctggttg ttgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga
    12181 gcaactagag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
    12241 gcagattgat cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa
    12301 atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag
    12361 tcagtcggtc aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga
    12421 cgtaccacat cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaggt
    12481 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
    12541 ttttccccag ccagttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat
    12601 tctcataacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
    12661 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
    12721 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
    12781 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
    12841 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa
    12901 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc
    12961 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
    13021 gaaaaacgat gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca
    13081 aacagaaaca accgacgcca ggcatccagg cgaagatgac accggtaccg gattgcggcg
    13141 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
    13201 attccggaat aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga
    13261 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat
    13321 gcggacgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
    13381 tggttcacga agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
    13441 aacaggttgc cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg
    13501 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
    13561 gtgcaagtat catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg
    13621 actatgcggc tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg
    13681 cggagaaagg tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa
    13741 tttccggcgg acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc
    13801 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga
    13861 gctacgtcac cgcagaagtg cacggtgtgt gcggcggcga gcatttaggt taaaaaaatg
    13921 cccggttgtg aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa
    13981 acgccttatc cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc
    14041 tgataagcgt agcgcatcag gcattgagca cctaatgccg gatgcggcgt gaacgcctta
    14101 tccggcctac aaacgcacga agattcaaca tgttgcaata gatattgtag gcctgatgag
    14161 cgtagcgcat caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc
    14221 tatcttcagg taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc
    14281 gacggtttta tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt
    14341 caacgccgta atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc
    14401 tgccttcacc gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc
    14461 aggcaagttc accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag
    14521 cagaaccaac atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc
    14581 gtcgaggttt tcgttaagat gcattgccat cggatattat cctgcgcgta atttttgtgc
    14641 gacacgaacc ggatgcgcag cggcgctggc ttccagatct aggtcacggc tttgcagcaa
    14701 caatacctgc gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa
    14761 tacgttatag ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag
    14821 agcttctgcg atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc
    14881 aataaagctg ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc
    14941 gccgatggtt gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacccg
    15001 acgctccaga cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga
    15061 cagttccagc tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa
    15121 atcagcggcg atatcgttgg cctggtttaa ggaacgcgct tcagccagca gttgctgctc
    15181 gcgcttaagg cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca
    15241 agtgactacg gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgtg
    15301 ctgatacata ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct
    15361 ccaggacgca agtcacaaaa tctgcgcata ataatatcaa aatgacgtcg aattgatagt
    15421 cgttctcatt actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca
    15481 gtaaaaaagt caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg
    15541 atgcgcccgc gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg
    15601 acatggcgga caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca
    15661 ctctcggcgc ggtaaatagc gtgattcagc gcgcttcttc gctggtcttt gacagtgtgg
    15721 aagccaaaaa gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg
    15781 gaacgttaac ccatttctcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct
    15841 gcgcgctatt tccctgcggg gcggcagcgg ttgctaattc cattcttgct tttgtcgaac
    15901 agggcgatca tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca
    15961 aaatcctcag caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata
    16021 tcgttaagca tctgcagcca aacactaaaa tcgtgtttct ggaatcgcca ggctccatca
    16081 ccatggaagt ccacgacgtt ccggcgattg ttgccgccgt acgcagtgtg gtgccggatg
    16141 ccatcattat gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg
    16201 gcatcgatgt ttctattcaa gccgccacca aatatctggt tgggcattca gatgcgatga
    16261 ttggcactgc cgtgtgcaat gcccgttgct gggagcagct acgggaaaac gcctatctga
    16321 tgggccagat ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag
    16381 gtgtgcgttt gcgtcaacat catgaaagca gtctgaaagt ggctgaatgg ctggcagaac
    16441 atccgcaagt tgcgcgagtt aaccaccctg ctctgcctgg cagtaaaggc cacgaattct
    16501 ggaaacgaga ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca
    16561 ataatgaaga gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt
    16621 ggggcgggta tgaatcgttg atcctggcga atcaaccaga acatatcgcc gccattcgcc
    16681 cccaaggcga gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg
    16741 tcgacgatct gattgccgat ctggacgccg gttttgcgcg aattgtataa cattgccact
    16801 tttggacaat tttgcagaca tttaattgta aaaagtctta aattgttgcg tccgggatca
    16861 aggcgtcccg gacgaatcag gagtacaata ggcagataaa ggcttaaacg ctgttccaca
    16921 ggaaagtcca tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc
    16981 gcgctggcgg atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt
    17041 ttagaaaacg gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca
    17101 ggcgcattga ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc
    17161 gcagcaagtc tgggctgctg gctaagttat attcaggggc gctggttagg gaataccaaa
    17221 acggtgaaag gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg
    17281 tttgaccgcc acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg
    17341 ctgctgccaa ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac
    17401 tggttaagtg gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg
    17461 attccgttcg ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt
    17521 gccttgttaa ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt
    17581 aacgcctgac gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg
    17641 catcgcccgc attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc
    17701 acggctaaat tgcgatgcgc tttcatagcc gacgcgcatc gctgctgcgc tggccttcat
    17761 gccgtcatgg atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa
    17821 cggcgaggtg ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc
    17881 ttctgccgcc agttgctcga cgctcaggtt ttcggtgtat ttattctcaa tccgtttcag
    17941 cacgcggcta atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc
    18001 gcaaggtccg gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg
    18061 cgcatccagt ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc
    18121 tgataacgtg gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc
    18181 gccaatgtcc atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc
    18241 cagcggcacc tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag
    18301 caggtattca ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc
    18361 ggaaaagaga attatgatgc caggctcgta catcaccggt gtacgtgcga aaggcgtctc
    18421 gccatacaac aaacgtacat cgggcaacag ttctgacaaa ctattttctt taattttcag
    18481 tttattaact ttatccgcca gcaagcggca aatctcttca cgtttcatat cgcgtaattt
    18541 cttaggaata atgcggcaat ttgattgtgc acaattttgt agcatttctc cagcactctg
    18601 gaggaatagg caagacattg gcagaaatga gcattgagag ccagggcgct ggcgatcaca
    18661 atgaaaaaca tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt
    18721 aatgaacaac tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc
    18781 tggtttacgc gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag
    18841 cgtgaaaaaa accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct
    18901 ggaatttggc ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact
    18961 ggttcgcgaa cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg
    19021 caccaaattt atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct
    19081 gcaaacgggc ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc
    19141 agcaaccggt tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa
    19201 gcaggcgttc cattctgccc atgttcagcc ggtatttgcc gtgctcgatc cggtttatac
    19261 ctacaccctg ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt
    19321 ggaacagtat gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat
    19381 tttgctgacg ctgatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt
    19441 gcgcgccaac gtcatgtggg cggcgacgca ggcgctgaac ggtttgattg gcgctggcgt
    19501 accgcaggac tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga
    19561 tcacgcgcaa acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa
    19621 gcgcgctaag ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgatga
    19681 tgagcgtatt gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc
    19741 gacccacctc tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact
    19801 ggaagagcac ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg
    19861 ccgtatatac gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt
    19921 tcgtccagac ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag
    19981 gaggaacgta tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag
    20041 ctgggactgg gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg
    20101 ttagaagtgg gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc
    20161 ggcaaagccc tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg
    20221 tggaacgacg accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag
    20281 cttgattata tcgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc
    20341 gaagcatgga aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg
    20401 tgcaacttcc agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg
    20461 ataaaccaga tcgaacttca tccgctgatg caacaacgcc agctacacgc ctggaacgcg
    20521 acacacaaaa tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc
    20581 gatcagaaag tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt
    20641 atccgctggc atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt
    20701 attgccgaaa actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcgaaatt
    20761 gcaaaactcg atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacat
    20821 gcaaattctc ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc
    20881 cctcgcgtcc cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca
    20941 tcatgcagag ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg
    21001 gagaggtgaa aaacagatga aaataatact tctgttttta gcagccctgg caagttttac
    21061 cgtacacgca cagcccccct cactgaccgt agaacaaaca gtccggcata tttatcagaa
    21121 ctataaatca gatgccactg ccccttattt tggtgaaacc ggagagcggg cgataacttc
    21181 tgcgcgtatt caacaggcgc ttaccctgaa cgacaatctt acgctgccgg gcaatattgg
    21241 ctggctggat tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag
    21301 cgttgcgata acccaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt
    21361 taaagatgat aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg
    21421 ggtcattgac gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa
    21481 tgaaaaaacg ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc
    21541 cgaactcttt gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc
    21601 ttaccgccag gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga
    21661 tgaagatatg caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga
    21721 gtcgctattt tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg
    21781 tattcatgtt cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca
    21841 gcggcgcgaa ggcaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact
    21901 taagcagatt gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa
    21961 ttagccggaa caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct
    22021 gttgttctgg caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcagctg
    22081 gcgagcaatg tgcaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca
    22141 ctacgcccac aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat
    22201 gctcaggact attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat
    22261 ccccggcagc atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg
    22321 tttcttcgca ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc
    22381 aggcgtctga cgctgggtcg ccatcggcgt atgtttcgtc aacgccggac gggtattgcg
    22441 gttctggcga cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg
    22501 acggctgcca atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca
    22561 gtttgccgga tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt
    22621 cggtacgaag acgtcttcgc tcttataacc aatcttcgcc agcgggtttt tcccggtgta
    22681 atacatggtg gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg
    22741 gaaacgatgc tttttcagcc acagcgccag attcaccata tcttcatcac gcgtaccggg
    22801 gtgcgcggag atgaaatagg ggatcagata ctgctcttta cctgcctgtt ttgagtaagt
    22861 atcgaacagc tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgataa
    22921 cggcccttct tcggtatgtt ccggggcaat cttcagataa ccgccgacgt gatgggtcgc
    22981 caactctttg atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat
    23041 gaggattttt ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc
    23101 gtggttagtg tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt
    23161 ttgttcagcg cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag
    23221 atcggaaatt acgcccgtaa aaccaggaac ggtgtcgcgg atcgcttcga tctcattaat
    23281 gatcgaatct tccgaacggc tctgaataat gcgcccttcg tgctcggtga tagaacagaa
    23341 agagcagccg ccaaagcagc cgcgcataat gttgaccgag aaacggatca tttcgtaagc
    23401 cggaatacgg gcattgccat aggccggatg cggcacgcgc ttgtacggca gcgcaaaaac
    23461 gctgtccatc tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc
    23521 accgtgtttt tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg
    23581 cgaagcatga gcgtacagga ctttatcgcc cttcactttc tcgaaagaag gcagcaatac
    23641 gtaggttttt tcccacggtt tcgggcgcgg cggctgcacg gttacagctt tcgcttcctg
    23701 ctttttcggc gcaacaggtt tgttatccgc gcacggcaga tcttcgccat acggatgcgg
    23761 gattgggtcg attttccctg gggtatcaag acgagtagaa tccacaccgc tccagccagc
    23821 cagcgcctct tttacgataa ttgcggtatt acgcacatcg cggatttcgc taattggctc
    23881 gcccatcgcc agacgatgcg ctacttccac cagcggacgt tcaccgttac caaacatcag
    23941 catgtcggct ttcgaatcca ccagcacgga acggcgcacg gtatcggacc agtaatcata
    24001 atgcgcggta cggcgcagac tagcctcaat accgccaagg atcaccggca catctttcca
    24061 cgcctcttta caacgctggg tataaaccag tgtggcgcga tccggtcgct tacccgcgac
    24121 gttatccggt gtgtaggcat cgtcatgacg taaacggcga tcggcggtat agcggttgat
    24181 catcgaatcc atgttgccag cagtaacacc aaaaaacaga ttcggtttac ccagacgcat
    24241 aaagtcgtct ttgctgctcc agtccggctg ggcgatgatc ccgacgcgaa agccctgcgc
    24301 ttccagcata cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc
    24361 agtaaccaaa atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat
    24421 cggcaaaaac ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc
    24481 gcgatccggt tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata
    24541 atcaaaggcc ggggattata agccggaacg aaagagaaat cgaaaggtat tccatactcg
    24601 ccctcctcgg gcgagtatga agattacggt accggattga ccaacagttg cccaatcgag
    24661 ccacggtccg ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa
    24721 ggctgaccga aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag
    24781 cctctgtctt ccgggaacgg catcgcgcca ttcacgttac ggatctggaa catcacgcct
    24841 tcaatatgga acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc
    24901 tgcgcggtga catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca
    24961 cccagactaa tatcgcgact gcgaatcggc gaaccggcca tgatttcagt tggcagcaag
    25021 cgcatcggaa gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc
    25081 agggtagaaa ccaaaatgct ggatggctca aagaagccac gaatacgatc aacaatgctc
    25141 gccgcttcgc cacaggtgat cgacacttca tcaccgttgc tcatatccac cagaatctcg
    25201 cggcgctcgc ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc
    25261 tgatcgccag aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga
    25321 gagttcgacg cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg
    25381 ctttgtacac cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg
    25441 tattctggcg taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc
    25501 acaccataat ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt
    25561 ccggcaaggc cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac
    25621 agagtagctg cgttctggcg aatgggcagt acgggtgccc agtcagcgtt tggcgacatc
    25681 atccgtgccg gaccgcccat cagcgggcct ggtacctgta gcccggccac cgtcattgag
    25741 acattttctg tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg
    25801 atagtcggcc ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta
    25861 aatgaccagt gcgcacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt
    25921 agcggcggaa cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg
    25981 cctgcacaaa gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt
    26041 gctccatgta aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca
    26101 gattgccgac ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg
    26161 cgattttttg ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc
    26221 catactgact gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc
    26281 gattaagatt aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg
    26341 ccgcaattgc cgcgtgaaat gctccagtct tgaacggtag caggccgcga ccacggctgc
    26401 gggttccttc cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt
    26461 ccgcaatggt gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta
    26521 accagtacaa ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg
    26581 tcggcggttg cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga
    26641 tatagatagc attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc
    26701 caaacagcgg cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac
    26761 gcgggctgaa aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg
    26821 taataataag acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt
    26881 gtacgtcacc tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt
    26941 gttaatcgca acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt
    27001 tgaatacgct gccggatgac gacttaacgt ttcatccggc gttccttgca agcgggagga
    27061 aacaggccct ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag
    27121 tcgatctcaa cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt
    27181 tcgccagtga ctttctgtaa ctcttccggg cgcagtttaa ttttgcgttt cccaacatga
    27241 atggtcagcg tgctttgcgg cggcagaacg tacaattgcg ccagaccatc ttctccacgc
    27301 gcggcttctg ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc
    27361 agatcgctta ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg
    27421 gaagcatctt caatcaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa
    27481 gccttacctg cacggttacg cgccaccaga tcgttaaagg tgcagacgaa accgtaaccc
    27541 gcatcggaag ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc
    27601 gccccaggcg gcaacgttaa tttgccggtg agtggttcgc cctgaccacg cgccgacggc
    27661 agcgtaatcg ggtcgatggc atagctacga ccggtggaat caacaaacac taccggttgg
    27721 ttgctcttac ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc
    27781 gcgtcgatat catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg
    27841 acaggttcag acggcagcat gtcgtgctcg ctcatcgctt tcgcttcttc gcgttcctgc
    27901 aacggcgaac gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc
    27961 aggttattca ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc
    28021 agctcactct gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc
    28081 agttcgagga tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat cagcgccggt
    28141 ttcggttcat cttcgttacg aatgatctca atcacttcgt cgatgttgag aaacgccacc
    28201 agcaaacctt ccaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg
    28261 cggcgcacgg tatcgcgacg gaacaccagc cattcggaga ggatttccag caggtttttc
    28321 accgccggac gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga
    28381 tcggtggtag cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc
    28441 acaatcacca gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc
    28501 ggcagctttt tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc
    28561 tgatgcggta atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcgcgcata
    28621 cgcactgaac cacgtccgtt ctcgtagatt ttacggatct cggcgcgcga agtgataatt
    28681 tccgcttcag tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtggtt
    28741 ttcggctggt cgattaatgc gattgccgcc tgagctactt cacgcaggtt atgcggtgga
    28801 atatcggtcg ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga
    28861 cgagcaggca gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc
    28921 gtcccctgcc ccagctcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg
    28981 taacgcattg ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca
    29041 accagcggat aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg
    29101 ctatcgccgt gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta
    29161 aatttggcgc tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca
    29221 ggtttaagac catcgccaat aaacggcaac gcacggtcca tgatcacgta catggagtag
    29281 tttaagtagg cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt
    29341 aattctgatt cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta
    29401 cattatctgc cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc
    29461 gacggcgata aaggcatcaa tgtgccttga tatacaactc tttgctgtag acttgatcgg
    29521 catagagggc agcacaaggt tgcgcccatg ccacaccact cggcatggga gtccgctccg
    29581 ggcgattcga gaagttgagg ttatgcgagt ccagggaagc ctaaagcatc aaagtagtcg
    29641 actgtaagta tacggttcag cccagtttag ctgtgtctcc accagtgccc cgcgcctcct
    29701 gccataagtg cggcttgctc atggataccc ccgagacggc ttacggcgca ccggataata
    29761 aatttcgcgt atacatggta ttccttaccg gagaaattat aagcccaaca acaatacgcc
    29821 tgtggcattg ttcgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc
    29881 cccatcacgc caacggcaca taaacatccg tttccagtcg ctgctctgtc accccttcgg
    29941 caagattggt gtagtgaaac ataatcggtg ctttacgcat tttctcgccg cttgcaggca
    30001 gccagtggcg aataatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacgtggc
    30061 gggccacggc ataacgtcca ccggtaagtt caccattgct cacaccataa cgattatcgg
    30121 gaatcggttc gctaacgctg ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg
    30181 cagggtcgtc ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct
    30241 ctttacgcca catgataaat ttcgccacac tgtagttgag caactcaggg ctacagcggt
    30301 gcggcaacat cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt
    30361 ttgtcatttt gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat
    30421 tacggattta ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg
    30481 acttcaccct gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt ccagcgtttg
    30541 tggtcgatat caacattgat agtaccgctg gcatctttaa acacgtagag atcgtcagag
    30601 atgcgttcaa cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg
    30661 ctttctacgg tcgttacgct gccgttcggc ccctggaatc ctccggcctg actttgcgtt
    30721 gccgatgggc cagaaaaacc gccctgctct gctgccatca ccggtgcgct gcacagggcc
    30781 attactgcga ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc
    30841 cgagcattta acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttagtgc
    30901 tgtacagagc gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa
    30961 aaaatgcgaa ttttactgat agaagatgac atgctgattg gcgacggcat caaaacgggc
    31021 cttagtaaaa tgggttttag cgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg
    31081 ctttatagcg cgccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt
    31141 cgcgatattt tgcgcgaatg gcgagaaaaa ggtcagcgtg agccggtact gatcctgacc
    31201 gcgcgcgatg cgctggagga acgtgtagaa gggctgcgtc tgggagctga cgattatctg
    31261 tgtaaacctt ttgcgttgat agaagtcgcc gccaggctgg aagctctgat gcgccgaacc
    31321 aacggccagg ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt
    31381 atcgccacgc tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa
    31441 ttactgatgc gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat
    31501 acctgggacg aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc
    31561 aaactcggta gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa
    31621 tgaaatttac ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt ttaattctgg
    31681 cctcggtgac ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg
    31741 atgaattgtt cgacacccaa ctgatgctgt ttgccaagcg gttaagtacg ctcgatctca
    31801 acgaaatcaa cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg
    31861 ttgatgacga tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaacg
    31921 atggcgataa cggagaagat attccttata gctatcaacg ggaaggtttt gctgacgggc
    31981 aactggtggg cgataaagat caatggcgtt ttgtctggat gacctcacct gatggcaaat
    32041 atcgcatcgt tgttggtcag gaatgggaat accgtgaaga catggcgctg gcgattgttg
    32101 ccgggcaatt gatcccgtgg ctggtcgcac tgccgattat gttaatcatc atgatggtac
    32161 tattgggtcg tgaactcgcg ccgctgaaca aactggcgct ggcgctgcgt atgcgtgacc
    32221 ctgactcgga aaaaccacta aacgcgactg gcgtacccag cgaagtgcgt ccgttggttg
    32281 agtcgctaaa tcaactgttc gcccgcacac atgcgatgat ggttcgtgaa cgacgcttta
    32341 cctccgacgc agctcacgaa ctacgtagcc cgttaacggc gctgaaagtg caaaccgaag
    32401 ttgcgcagct ttctgacgat gatccgcaag cgcggaaaaa agcgctgctt caattacatt
    32461 acgggatcga tcgcgctact cgtctggttg atcaactgct cactctatcg cggctggact
    32521 cactggataa ccttcaggac gtcgcggaga tcccgcttga agatctcctg caatcgtcgg
    32581 tgatggatat ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg
    32641 cccacggcat caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc
    32701 tggataacgc cgtgcgctac agtccacagg gcagcgtgat agacgtcacg ctgaatgctg
    32761 ataatttcat cgtgagggat aacggccccg gtgtgacgcc agaggctctg gcgcgaattg
    32821 gcgaacgctt ctatcgcccc cccggacaaa ccgctaccgg cagtggactt gggctatcga
    32881 ttgtccagcg aatcgccaaa ttgcatggca tgaatgttga gtttgggaat gcggaacaag
    32941 gtggatttga ggcgaaggta agctggtaac ttctctgtct atgagagccg ttaaaacgac
    33001 tctcatagat tttactaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa
    33061 accagacaaa taccgagcga gaatacatat acccaaaact cgaacatttc ccgcataaag
    33121 agttttctta agataagaat aataagtggc gtaagaagaa aaaatgctgc aatcattgct
    33181 tgcaatatac gatcttcgcc tgaaacggaa ggtatactgc aaatattgtc tcccgactca
    33241 agcatccagt catacttatt gaaagcaaaa aaagcatcta ttaaccaata agtaagcatt
    33301 atgtataaca gagcttcaaa tattgtttga attttatgct ttaacatata tcagtacgct
    33361 cataaaaatc catttataat tatcaacatg acacgttgat attttcatct tttacttatt
    33421 caaagccata ttttcaatcc caaaaaaaac cgaaccaaac gtgtaaatta ttccggtgcg
    33481 agtaagaatt atgaggaatg gctatcagta ttgtcatttt cagaaaatat ttatcctgca
    33541 tcggtgagtc agagcaagat cagacttttg ctaaattcgc aaaagacttt gcacattttg
    33601 ctaatttcac cgtaccgctc tgtgacgtac tatagtcggc aaacgtctca ccttgaggtt
    33661 aaaaaatgag caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc
    33721 aactgaacga caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg
    33781 tccgcatcgt tcgcgccgac agcgactacg atgtcaaagc ggaagtacaa aactttctct
    33841 gggctgatgt ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga
    33901 aaaaatatat tgatgatgta ttcaccgaag gtcatggaac gctgtatgcc agcgatggcc
    33961 gtacccgcaa agatccgtca aaaaaatacg gttccggcgg cctggtacag ggcaaaaaat
    34021 atatgctttc tctgacctgg aacgcaccaa tggaagcctt caccgaaaaa gatcaattct
    34081 tccacggcgt tggcgtcgac ggtgtgtatc tgccgttcca taaagcaaac caattccttg
    34141 gcatggaacc gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc
    34201 gctatactga agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc
    34261 tttagaagga gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac
    34321 atcaccgtca ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag
    34381 aaggttgcca cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta
    34441 tggcaccgga ttctatcgtg atgattgagc agtgggaaag catcgcgcat cttgaagcgc
    34501 atctgcaaac cccgcacatg aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga
    34561 tgaatatccg catcctgcaa cctgggattt aaacctgcct tgtttgcccg gccatcctga
    34621 ccgggcaatg ttctttcctt taaacctcaa tctccgccat gtcgcctttc tcttgcaacc
    34681 agttgcggcg atcttccgag cgtttcttcg ccagcagcat atccatcatc gcgtcagtac
    34741 gctgatcgtc ttcatcatcg atagtcaact gcaccagacg gcgagtgttc ggatcaagcg
    34801 tggtttcacg caattgcatc gggttcattt cccccagacc tttaaaacgc tggacgttcg
    34861 gcttgccttt cttgcgtttt aattgctcaa gtacgccctc tttctcttct tccgtcagcg
    34921 cgtaataaac ctctttcccg agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt
    34981 gaccgtgttt caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa
    35041 tgtgcagacc atcagagtcc gcatccgcga ggatacagat tttgccataa cgaagctggc
    35101 tcagatcgtc gctgtcagga tcgataccga tcgctaccga aatatcatgc acttcctgcg
    35161 aggccagcac ttcgtcggaa gaaacttccc aggtgttaag gatcttacct ttcagtggca
    35221 tgatcgcctg atattcgcga tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt
    35281 ccacaaggaa cagttcggta cggttaagat cctgcgcggt acagtcagcc agtttgccag
    35341 gcagtgctgg gccgctggtc agctttttgc gcaccacttt tttagccgca cgcatacggc
    35401 gctgggcgct ggaaatcgcc atctccgcca gcagctccgc cgcctgaacg ttctggttca
    35461 gccacaggat aaaggcatct ttcaccacgc ctgaaacgaa tgccgcgcat tgacgcgaag
    35521 agagacgctc tttggtctgc ccggcaaact gcggatcctg cattttcacc gacagaacat
    35581 aggcgcagcg atcccagata tcttccgccg acagcttcac gccgcgcggc agaatgttgc
    35641 ggtattcaca gaactcacgc atcgcgtcca acaggccctg acgcagacca ttaacatggg
    35701 taccgccctg catcgttggg ataaggttga cgtagctttc ggtcagcagt tcaccgcctt
    35761 ccggcagcca cagtagcgcc cagtccacag cttcagtatc gccagcgaaa ttaccgataa
    35821 acggtttttc tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac
    35881 cgtcctgata gcaccagcgc tgttcggtgt tgttgatctc atctttaaag gtaatctcaa
    35941 cgccggggca cagtactgct ttggccttca gcacatgcgt caggcgtgaa acagaaaaac
    36001 gcgggctgtc aaagaaggtt tcatccggcc agaagtgcac gctggtaccg gtattgcgtt
    36061 taccgcaagt gccgacaacc tgtaaatcct gcaccttttc gccattttca aaggcgatgt
    36121 tataaacctg accatcgcgg cgcacgttaa cttctacgcg cttcgacagg gcgttaacca
    36181 ccgaaatccc cacgccatgc aggccgccag agaactggta atttttgtta gagaatttac
    36241 cgcctgcatg cagacggcaa agaatcagtt caaccgccgg tacaccctct tccgggtgaa
    36301 tatccaccgg catcccgcgc ccatcgtcaa taacttctaa cgactggtca gcatgtaaga
    36361 ttacatccac gcgttttgcg tgacccgcca gtgcttcatc gacactgtta tcaataactt
    36421 cttgccccaa atggttaggg cgagtggtat cggtatacat ccccggacgg cggcgaaccg
    36481 gctcaagccc ggtgagtacc tcaatggcat cagcgttata agtttgcgtc atggtttaag
    36541 ttagcaattc gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg
    36601 aaataatctt cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag
    36661 gaagcgtagt acgccaccgc ctggcggtaa tccagcactt catctcccgt ctgttgcagc
    36721 agccagatca aatccggcgc ttccagcggg tcaatctgca tgactttgag atcgtaaata
    36781 tggcgtgact ctagcacata ttgctgcccg gtgtaggggt tctcgttctg accgagatag
    36841 tccgtcagca gttcaaacgg gcgcaccgcc gagtttacca ccactgcggg cagcataaaa
    36901 cattgtgaca accaggtggc gtaatatccc cccagtgacg aaccgacaat acccagcgaa
    36961 tcgccgccat gttccaggac aatggattcc agcagctctg ccgcgtcgga aggatacggc
    37021 ggcaactgcg gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca gttttttaac
    37081 aagctcgctt ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac
    37141 atcagtagcc ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct
    37201 cggtggtcag cgtgccatca gcatgtaact cgagagtacg ccagccgggc gcgatggtgt
    37261 ccagcgtaaa gttggaacag tgcggcttaa actgcacaca ggtcgacggc gttgccagca
    37321 ggcggcgacc gttccagtcg agatccagct cctgatgaat atgaccgcac agcaagtatt
    37381 tgacgtgcgg aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc aggctgtgtt
    37441 gatcaagcca actacaaccc gcaggtagcg gatgatgatg cagcagcagc aacgtatggc
    37501 gttctggcgc atcggccagt ttacgttcca gccactcaag ctgaaactcg ctcagctcac
    37561 cgtgcggcac gccaaacacc tggctatcca gcaacaggat ttgccattgc tcaccaataa
    37621 acacccgctt cgccggggag atacccgcat cctgtaacgc gctgtacatc gcgggctgga
    37681 aatcgtggtt gccaggcagc cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga
    37741 aatgccgata ggccgcagag gattgatcct gcgctaaatc acctgtcgcg acaatcaggt
    37801 cgaattcgtg ctggtgtgga cgaatcgcct ccagcaccgc ctggtagctt tcccaggtat
    37861 ttacccctaa cagggcttcg tgcttttgtg caaacaggtg agtatcggta atttgtaaaa
    37921 tcctgactct ggcctcacca gccagaggaa gggttaacag gctttccaaa tggtgtcctt
    37981 aggtttcacg acgctagtaa accggaatcg ccatcgctcc atgtgctaaa cagtatcgca
    38041 accagtccgc taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc aactttttat
    38101 taggataatc ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac acttcagcca
    38161 ccatcgcgtc atgatacaga cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg
    38221 gcgcagtctg ttctattgtc accagggtag tgtatcgggt cgattccaca atcgtcagcc
    38281 gatattgtgc gtttgccacc tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta
    38341 acaaacggcg caattgtgaa aagttcatct cgcacaggcg catcatttca ggaaagtcag
    38401 gtgtgtaacg cttcatttat gcccactcat tttttaacgc ttgatgatgc agctgcagcc
    38461 attgcaaagc gatgaccgac gctgcgttgt cgattttccc ctcttctacc cactggtatg
    38521 cctgttcccg gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa
    38581 taccgcttgc ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc tcactggtgc
    38641 cccccgggct tgccaggaaa cttaacaccg gtttggtccg tttgactatc agtcccgcct
    38701 cttcaatcgc ttcgcgacgg gcaacatctt ccacactttc accctcttct atcatcccgg
    38761 caaccatctc cagtagccag ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct
    38821 caatcagcac aacttcatca cgcactgggt caaagggtag caagactgcg gcgtgaccgc
    38881 gctcaaaaat ttcccgccgt acctcatgac tcatttgccc gttgaataga cgatgacgaa
    38941 atctataaag atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca ataatttcta
    39001 catcgttttt gccaaatgta acgggcaggt tgtctggctt aagcattgtt aatgtcctgg
    39061 cactaatagt gaattaaatg tgaatttcag cgacgtttga ctgccgtttg agcagtcatg
    39121 tgttaaattg aggcacatta acgccctatg gcacgtaacg ccaacctttt gcggtagcgg
    39181 cttctgctag aatccgcaat aattttacag tttgatcgcg ctaaatactg cttcaccaca
    39241 aggaatgcaa atgaagaaat tgctccccat tcttatcggc ctgagccttt ctgggttcag
    39301 ttcgttgagc caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc
    39361 ggaattgcgt aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg
    39421 cagtccatta ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg
    39481 cgacgcgaac ggcatcaact ctaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat
    39541 ttttgatatg tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag ggattcagga
    39601 cgtcacatat cagaccgatc agcaaacctt gatcctcaac accgcgaccg cttatttcaa
    39661 cgtgttgaat gctattgacg ttctttccta tacacaggca caaaaagaag cgatctaccg
    39721 tcaattagat caaaccaccc aacgttttaa cgtgggcctg gtagcgataa ccgacgtgca
    39781 gaacgcccgc gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac gcaataacct
    39841 tgataacgcg gtagagcagc tgcgccagat caccggtaac tactatccag aactggcagc
    39901 gctgaatgtc gaaaacttta aaaccgacaa accacagccg gttaacgcgc tgctgaaaga
    39961 agccgaaaaa cgcaacctgt cgctgttaca ggcacgcttg agccaggacc tggcgcgcga
    40021 gcaaattcgc caggcgcagg atggtcactt accgacgctg gatttaacgg cttctaccgg
    40081 gatttctgac acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga
    40141 cgacagcaat atgggccaga acaaagtggg cctgagcttc tcgctgccga tttatcaggg
    40201 cggaatggtt aactcgcagg tgaaacaggc acagtacaac tttgtcggtg ccagcgagca
    40261 actggaaagc gcgcatcgta gcgtcgtgca aaccgtacgt tcctccttca acaacattaa
    40321 tgcatctatc agtagcatta acgcctacaa acaagccgta gtttccgctc aaagctcatt
    40381 agacgcgatg gaagcgggct actcggtcgg tacgcgtacc attgttgatg tgttggatgc
    40441 aaccaccacg ctgtacaacg ctaagcaaga gctggcaaat gcgcgttata actacctgat
    40501 taatcagctg aatattaagt cagccctggg tacgttgaac gagcaggatc tgctggcact
    40561 gaacaatgcg ctgagcaaac cggtttccac taatccggaa aacgttgccc cgcaaacgcc
    40621 ggaacagaat gctattgctg atggttatgc gcctgatagc ccggcacccg tcgttcagca
    40681 aacatccgca cgcactacca ccagtaacgg tcataaccct ttccgtaact gatgacgacg
    40741 acggggcttc ggccccgtct gaacgtaagg caacgtaaag atacgggtta tctgccgcat
    40801 tcttccccct tctcgcttca atttcgacca gccatcctct attctgatgg gtatttacca
    40861 ctggtcccgg aagacaaaaa tgaaacggac aaaatccata cgccacgcat cgttccgcaa
    40921 aaactggagc gcgcgccatc tgacaccagt cgctctcgcg gttgccactg tttttatgct
    40981 ggctggctgt gaaaagagtg atgaaacagt gtctctctat caaaatgctg acgactgttc
    41041 agctgcaaac ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga
    41101 agccgaacgt actgcgccga aatacgccac ccgtgaagac tgtgttgctg aatttggtga
    41161 aggtcagtgc cagcaggcac cagcccaggc tggcatggca ccagaaaacc aggcgcaggc
    41221 ccagcaatcc agcgggagtt tctggatgcc gctgatggcc ggttacatga tggggcgtct
    41281 gatgggcggc ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa acccagccag
    41341 cccggcttac ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag cccagccagg
    41401 ccgcaccatg accgtaccga agacagccat ggcaccaaaa ccggcgacca ccactaccgt
    41461 tacccgtggc ggttttggtg aatctgttgc caaacaaagc actatgcagc gtagcgcaac
    41521 tggcacctct tctcgttcaa tgggtggctg ataccgatgg aaagagtcag tattaccgag
    41581 cgcccggact ggcgtgagaa agcccacgaa tacggtttca attttcacac catgtacggc
    41641 gagccgtact ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt tgaaaagctg
    41701 gaagaagtca ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc
    41761 agcgatgagc tgatgaccaa attccgcatt ccaaaacaca cctggagttt tgtgcgccag
    41821 tcatggctga cgcaccagcc atcgctttat tcgcgtcttg atctggcgtg ggatggcact
    41881 ggcgaaccta aacttctgga aaataacgcc gatacgccaa cgtcactata cgaggcggcg
    41941 ttctttcagt ggatctggct ggaagatcag cttaacgccg gtaacttgcc ggagggcagc
    42001 gaccagttta acagtctgca agaaaaactg atcgatcgct tcgttgagct gcgtgaacag
    42061 tatggcttcc agttgctgca tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc
    42121 attcagtatt tgcaggactg cgcaacggaa gctgaaattg ctactgagtt cctctacatc
    42181 gatgatatcg ggttaggtga aaaaggtcag ttcacggatt tacaggatca ggtaatttcc
    42241 aacctgttca agctgtatcc gtgggaattt atgttgcgtg agatgttttc caccaagctg
    42301 gaggatgcag gcgtacgctg gctggaaccg gcgtggaaga gcattatctc caacaaggca
    42361 cttctaccgc tactgtggga gatgttcccg aatcacccga acctgctgcc cgcttatttt
    42421 gcggaagatg atcatccgca aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa
    42481 ggcgcaaacg tgtcgatcat tgaaaacggc aaaaccattg aagcagcgga aggtccgtat
    42541 ggcgaagaag ggatgattgt tcagcaattc cacccgttac cgaaattcgg cgacagctat
    42601 atgctgattg gtagctggct ggtgaacgat caacccgccg gaattggcat tcgtgaagac
    42661 cgtgcattga tcacccagga tatgtctcgg ttttatccac atatttttgt tgagtaatca
    42721 atgatgccgg atggcattcg ccatccggta attgttaacc tatctgcacc gacagcatac
    42781 tcaggctgcc catttctata ccatcgaccg gaatggtaat tggctcctgc ccatcccacg
    42841 cacctaagac atacaacaac ggcagatagt gctccggcgt tgggttcgat aacgcgccac
    42901 cttcatggtc gaggtaattc accagaggat gctgctctac cgccccttgc cacgtcagat
    42961 tcgctttcac atactcatta aacgacatcg cccaaggata tggtgaacta tcaccgtgcc
    43021 acttcactgt acgcaggtta tgcaccacgt tgccactggc gaccaacatt attccttcat
    43081 ctcgcagcgc tgccagtttg cgccccattt cgaaatgcca ggcggcaggt ttgctactgt
    43141 cgatactcaa ctgcaccatc gggatatcgg cgtcaggata catcttaatc agcacgcccc
    43201 aggaaccgtg gtcaaagccc caggcttctt tatccagcgt caccgggacc ggtgctaaca
    43261 gctcaaccag atgctgtgcc agcacaggcg aacccggagc aggataatgc gtatcgtaca
    43321 gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc
    43381 ctgttccacg ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg
    43441 tcatccccaa cttctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac
    43501 taccgtgacc taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta
    43561 aaggtgtcat tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac
    43621 aatgatgatg atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt
    43681 tgtcgatgtc agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg
    43741 cgtttctcgg cgttctcggg caaaaaccct cgaaccgctt actggcgttt tcgctaggat
    43801 ttgcggcggg gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcactagcag
    43861 ctgaaggaat gtcgcctgtg ttgggttatg gaatgtttat cttcggtctg cttggctatt
    43921 ttggcctgga ccgcatgttg ccacatgcgc atccgcagga tttaatgcaa aaatcggtgc
    43981 agccgttgcc aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc
    44041 ataacttccc ggaagggatt gccacctttg tcacggcgag cagcaacctg gagctgggat
    44101 ttggcatcgc actggccgtc gcgttgcaca atattcctga aggtctggcg gttgcaggcc
    44161 cggtttatgc agcaacgggt tctaaacgca cggcaattct gtgggcgggg atttctggat
    44221 tggcagaaat tcttggtggt gtgctggcgt ggttaatcct aggtagcatg atttcccccg
    44281 tggtcatggc ggcaatcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg
    44341 aattaatgcc gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat
    44401 gtggaatgtc agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa
    44461 agcaacatca gaagttggcc agcgcatgga caacttctgc tagcttcact aaagccttat
    44521 tctacctttt cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc
    44581 agtgcgtctc tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc
    44641 accacaacaa gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta
    44701 atatttaaca atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt
    44761 catatggtcg cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc
    44821 tgatattaat gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg
    44881 caaaataaaa accgaagcca gctacaactt cggtttcttg attaaggcag taattaagca
    44941 gcggttttca gctggcttta cgctcatgtg cctgacggta tgccaccagg tcttcaatag
    45001 tcacgagcgc catattgtgt ttattggcaa actcaatgca ctctggtgca cgcgccatcg
    45061 tgccatcgtc attagtcaac tcacacagca caccagccgg tttaaagcct gccagcgtca
    45121 tcaaatcaat agtcgcttca gtatgaccgc cacgcgtcag tacaccacct gcctgcgcgc
    45181 gaagtgggaa aacgtggccc gggcgattca gatcagaagg ttttgcgcca tcggcaatcg
    45241 ctgcgcgaac ggtcgtaatt cgatcagcgg cagaaacacc ggtagtcaca ccttcagccg
    45301 cttcaatggt cacggtaaaa ccggtgccat aggcgctggt gttattttct accatcattg
    45361 gcagatcaag ttgtttacgg cgatcttcag tgatgcacag gcaaacaata ccgctaccgt
    45421 ggcgaatggt cagcgccatc tgctcaacgg tcatggtttc tgccgggaag atcatatcgc
    45481 cttcgttttc acggtcttca tcatcaagca ccattacacc gcgtccttca cgcagcgcag
    45541 ccagtgcatt ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca
    45601 tggtaaaaaa acctcactaa aattatggtt accagaatca gggcagtctt aggagtggcg
    45661 gcatatagcc aaaataacgt gagcgggtcc atgcccgaca gaatcgttac tctctcccat
    45721 ccggactcta accgtcggcc ccggaattac accggatctg ctgtcctttg agttcgcacc
    45781 caaagcgctc gcgggctttc aactgagttg atttaccgcc ggtggggaat ttcgccccgc
    45841 cctgagaata agcggattca ctataacgct aatgattagc ggcagcaacg catagcttca
    45901 cataattctg gtttatgact tacccttatc gcactacaat ggcactcaac acctatcagt
    45961 acagggaaac tacaatgatt gacccgaaaa aaattgagca aatcgctcgc caggttcacg
    46021 aatcaatgcc taaaggaatc agggagttcg gggaagatgt ggagaaaaaa atccgccaaa
    46081 ccctacaagc tcagctgacg cgcctcgacc ttgtaagccg tgaagagttc gacgtccaaa
    46141 cgcaagtcct gttacggact cgtgagaaac tggcgctgct tgaacaaaga atgagcgaac
    46201 tggaaaatcg cagtactgag attaaaaagc agccagatcc agagactctc cccccaacgc
    46261 tgtaaacatt tgtctttaat gcactgtaaa ttatgaatta atcct
//
LOCUS       DAZGTR010000021        45601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGTR010000021 DAZGTR010000000
VERSION     DAZGTR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.021"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            226..1590
                     /gene="yajR"
                     /locus_tag="ACHLCY_001380"
     CDS             226..1590
                     /gene="yajR"
                     /locus_tag="ACHLCY_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0368676.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSELTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1718..2209)
                     /gene="yajQ"
                     /locus_tag="ACHLCY_001381"
     CDS             complement(1718..2209)
                     /gene="yajQ"
                     /locus_tag="ACHLCY_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP0368677.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2377..3288
                     /gene="panE"
                     /locus_tag="ACHLCY_001382"
     CDS             2377..3288
                     /gene="panE"
                     /locus_tag="ACHLCY_001382"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP0368678.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANAPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3251..3841
                     /gene="yajL"
                     /locus_tag="ACHLCY_001383"
     CDS             3251..3841
                     /gene="yajL"
                     /locus_tag="ACHLCY_001383"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP0368679.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3895..5343)
                     /gene="thiI"
                     /locus_tag="ACHLCY_001384"
     CDS             complement(3895..5343)
                     /gene="thiI"
                     /locus_tag="ACHLCY_001384"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP0368680.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5549..5791
                     /gene="xseB"
                     /locus_tag="ACHLCY_001385"
     CDS             5549..5791
                     /gene="xseB"
                     /locus_tag="ACHLCY_001385"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP0368681.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5791..6690
                     /gene="ispA"
                     /locus_tag="ACHLCY_001386"
     CDS             5791..6690
                     /gene="ispA"
                     /locus_tag="ACHLCY_001386"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP0368682.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6715..8577
                     /gene="dxs"
                     /locus_tag="ACHLCY_001387"
     CDS             6715..8577
                     /gene="dxs"
                     /locus_tag="ACHLCY_001387"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP0368683.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8757..9731
                     /gene="yajO"
                     /locus_tag="ACHLCY_001388"
     CDS             8757..9731
                     /gene="yajO"
                     /locus_tag="ACHLCY_001388"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP0368684.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9785..10303)
                     /gene="pgpA"
                     /locus_tag="ACHLCY_001389"
     CDS             complement(9785..10303)
                     /gene="pgpA"
                     /locus_tag="ACHLCY_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP0368685.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSSIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10281..11258)
                     /gene="thiL"
                     /locus_tag="ACHLCY_001390"
     CDS             complement(10281..11258)
                     /gene="thiL"
                     /locus_tag="ACHLCY_001390"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP0368686.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11336..11755)
                     /gene="nusB"
                     /locus_tag="ACHLCY_001391"
     CDS             complement(11336..11755)
                     /gene="nusB"
                     /locus_tag="ACHLCY_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP0368687.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11775..12245)
                     /gene="ribE"
                     /locus_tag="ACHLCY_001392"
     CDS             complement(11775..12245)
                     /gene="ribE"
                     /locus_tag="ACHLCY_001392"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP0368688.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12334..13437)
                     /gene="ribD"
                     /locus_tag="ACHLCY_001393"
     CDS             complement(12334..13437)
                     /gene="ribD"
                     /locus_tag="ACHLCY_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP0368689.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13441..13890)
                     /gene="nrdR"
                     /locus_tag="ACHLCY_001394"
     CDS             complement(13441..13890)
                     /gene="nrdR"
                     /locus_tag="ACHLCY_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP0368690.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14041..14580
                     /gene="yajI"
                     /locus_tag="ACHLCY_001395"
     CDS             14041..14580
                     /gene="yajI"
                     /locus_tag="ACHLCY_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0368691.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14879..15763
                     /gene="tsx"
                     /locus_tag="ACHLCY_001396"
     CDS             14879..15763
                     /gene="tsx"
                     /locus_tag="ACHLCY_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP0368692.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15940..16287)
                     /gene="yajD"
                     /locus_tag="ACHLCY_001397"
     CDS             complement(15940..16287)
                     /gene="yajD"
                     /locus_tag="ACHLCY_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP0368693.1"
                     /translation="MAIIPKNYARLESGYREKALKIYLWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16416..17387)
                     /gene="secF"
                     /locus_tag="ACHLCY_001398"
     CDS             complement(16416..17387)
                     /gene="secF"
                     /locus_tag="ACHLCY_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP0368694.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17398..19245)
                     /gene="secD"
                     /locus_tag="ACHLCY_001399"
     CDS             complement(17398..19245)
                     /gene="secD"
                     /locus_tag="ACHLCY_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP0368695.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19273..19605)
                     /gene="yajC"
                     /locus_tag="ACHLCY_001400"
     CDS             complement(19273..19605)
                     /gene="yajC"
                     /locus_tag="ACHLCY_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP0368696.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19628..20755)
                     /gene="tgt"
                     /locus_tag="ACHLCY_001401"
     CDS             complement(19628..20755)
                     /gene="tgt"
                     /locus_tag="ACHLCY_001401"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP0368697.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20811..21881)
                     /gene="queA"
                     /locus_tag="ACHLCY_001402"
     CDS             complement(20811..21881)
                     /gene="queA"
                     /locus_tag="ACHLCY_001402"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP0368698.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIIHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21974..22555
                     /gene="acpH"
                     /locus_tag="ACHLCY_001403"
     CDS             21974..22555
                     /gene="acpH"
                     /locus_tag="ACHLCY_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP0368699.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22560..24377)
                     /gene="malZ"
                     /locus_tag="ACHLCY_001404"
     CDS             complement(22560..24377)
                     /gene="malZ"
                     /locus_tag="ACHLCY_001404"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP0368700.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFDGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHRQLTDGILALPAISATVWMN"
     gene            complement(24533..25906)
                     /gene="proY"
                     /locus_tag="ACHLCY_001405"
     CDS             complement(24533..25906)
                     /gene="proY"
                     /locus_tag="ACHLCY_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP0368701.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25982..27301)
                     /gene="brnQ"
                     /locus_tag="ACHLCY_001406"
     CDS             complement(25982..27301)
                     /gene="brnQ"
                     /locus_tag="ACHLCY_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP0368702.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27708..29003)
                     /gene="phoR"
                     /locus_tag="ACHLCY_001407"
     CDS             complement(27708..29003)
                     /gene="phoR"
                     /locus_tag="ACHLCY_001407"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP0368703.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFIQGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWLRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29061..29750)
                     /gene="phoB"
                     /locus_tag="ACHLCY_001408"
     CDS             complement(29061..29750)
                     /gene="phoB"
                     /locus_tag="ACHLCY_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP0368704.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPPSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29940..31142
                     /gene="sbcD"
                     /locus_tag="ACHLCY_001409"
     CDS             29940..31142
                     /gene="sbcD"
                     /locus_tag="ACHLCY_001409"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP0368705.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31139..34282
                     /gene="sbcC"
                     /locus_tag="ACHLCY_001410"
     CDS             31139..34282
                     /gene="sbcC"
                     /locus_tag="ACHLCY_001410"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP0368706.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQRIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDKQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASETLEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34408..35592
                     /gene="araJ"
                     /locus_tag="ACHLCY_001411"
     CDS             34408..35592
                     /gene="araJ"
                     /locus_tag="ACHLCY_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP0368707.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAELFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35736..36644)
                     /gene="mak"
                     /locus_tag="ACHLCY_001412"
     CDS             complement(35736..36644)
                     /gene="mak"
                     /locus_tag="ACHLCY_001412"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP0368708.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36769..37680
                     /gene="rdgC"
                     /locus_tag="ACHLCY_001413"
     CDS             36769..37680
                     /gene="rdgC"
                     /locus_tag="ACHLCY_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP0368709.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMESPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37758..37841)
                     /locus_tag="ACHLCY_001414"
     CDS             complement(37758..37841)
                     /locus_tag="ACHLCY_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368710.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37838..38179)
                     /locus_tag="ACHLCY_001415"
                     /pseudo
     CDS             complement(37838..38179)
                     /locus_tag="ACHLCY_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38327..38611)
                     /gene="ppnP"
                     /locus_tag="ACHLCY_001416"
     CDS             complement(38327..38611)
                     /gene="ppnP"
                     /locus_tag="ACHLCY_001416"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP0368711.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38683..39360)
                     /gene="aroM"
                     /locus_tag="ACHLCY_001417"
     CDS             complement(38683..39360)
                     /gene="aroM"
                     /locus_tag="ACHLCY_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP0368712.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39618..39809)
                     /gene="yaiA"
                     /locus_tag="ACHLCY_001418"
     CDS             complement(39618..39809)
                     /gene="yaiA"
                     /locus_tag="ACHLCY_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP0368713.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39859..40383)
                     /gene="aroL"
                     /locus_tag="ACHLCY_001419"
     CDS             complement(39859..40383)
                     /gene="aroL"
                     /locus_tag="ACHLCY_001419"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP0368714.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40566..41024)
                     /gene="yaiI"
                     /locus_tag="ACHLCY_001420"
     CDS             complement(40566..41024)
                     /gene="yaiI"
                     /locus_tag="ACHLCY_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP0368715.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41144..41953
                     /gene="proC"
                     /locus_tag="ACHLCY_001421"
     CDS             41144..41953
                     /gene="proC"
                     /locus_tag="ACHLCY_001421"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP0368716.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(41970..43085)
                     /gene="adrA"
                     /locus_tag="ACHLCY_001422"
                     /gene_synonym="dgcC"
     CDS             complement(41970..43085)
                     /gene="adrA"
                     /locus_tag="ACHLCY_001422"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP0368717.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43187..43507)
                     /gene="psiF"
                     /locus_tag="ACHLCY_001423"
     CDS             complement(43187..43507)
                     /gene="psiF"
                     /locus_tag="ACHLCY_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP0368718.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSYCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43626..45041)
                     /gene="phoA"
                     /locus_tag="ACHLCY_001424"
     CDS             complement(43626..45041)
                     /gene="phoA"
                     /locus_tag="ACHLCY_001424"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP0368719.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45142..45402)
                     /gene="iraP"
                     /locus_tag="ACHLCY_001425"
     CDS             complement(45142..45402)
                     /gene="iraP"
                     /locus_tag="ACHLCY_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP0368720.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT        11268 a        12196 c        11461 g        10676 t
ORIGIN      
        1 aaaaatgcac atagcaatca agaaatgata ttattacttt tttgatgtaa aactatttag
       61 ttttaaaatt cattttttca acatatagag cattcatttt atgaatgcaa aaaaacatac
      121 ctgacacaca aattactctc ctctgtaata ttttctcatt aacactctgt ttacccaaca
      181 tgcgctccgc actacactat gtgggcattt ttttaagggg tggtaatgaa cgattataaa
      241 atgacgccag gtgagaggcg cgcgacctgg ggtttaggga ccgtattctc gttgcgcatg
      301 ctgggcatgt ttatggttct gccggttctg accacgtacg gcatggctct gcaaggtgcc
      361 agcgaagcat taatcggtat tgccattggt atttatggtc tgactcaggc cgtttttcag
      421 attccgtttg gcctgctttc cgaccgcatt ggtcgcaaac cattaattgt cggtgggctg
      481 gcggtgtttg ccgccggtag cgttatcgct gcgctttccg actccatctg gggaattatt
      541 ctgggccggg cgctacaagg ctctggagca attgccgccg ccgttatggc gctgctttcc
      601 gagctcacgc gcgaacaaaa ccgcaccaaa gcgatggcgt ttatcggcgt gagctttggc
      661 attacctttg ccattgcgat ggtgcttggc ccgatcatca ctcacaaact tgggctgcac
      721 gcgctgttct ggatgatcgc tattctggca acaaccggca ttgcgttgac catttgggtt
      781 gtgcccaaca gtagcactca cgtacttaat cgtgagtccg gaatggtgaa aggcagtttc
      841 agtaaagtgc tggcggaacc gcggctgcta aaactcaact ttggcattat gtgtctgcac
      901 atgcttctga tgtctacgtt tgttgccctg cccggacagc tagctgatgc ggggttcccg
      961 gcggctgaac actggaaggt ctatctggcg acgatgttaa tcgcctttgg ctcggtcgtg
     1021 cctttcatta tctacgctga agttaagcgc aaaatgaagc aagtctttgt cttctgcgtc
     1081 gggttgatcg tggttgcgga aattgtgttg tggaacgcac aaacgcagtt ctggcaactg
     1141 gtggtcggcg tgcagctttt ctttgtggcg tttaatttga tggaagccct tctgccctca
     1201 cttatcagta aagagtcgcc agcaggttac aaaggtacgg cgatgggtgt ctactccacc
     1261 agccagtttc ttggcgtggc gattggcggt tcgctgggcg gctggattga cggcatgttt
     1321 gacggtcagg gggtatttct cgctggcgca atgctggccg cagtgtggct ggcagtcgcc
     1381 agtaccatga aagaaccgcc gtatgtcagc agtttgcgca ttgaaatccc ggcggacatt
     1441 gccgcaaacg aagcgttaaa agtgcgtttg ctggaaactg aaggcgtcaa agaagtgttg
     1501 attgcagaag aagaacattc agcttatgtg aaaatcgaca gcaaagtgac gaatcgcttt
     1561 gagatagaac aggcaattcg tcaggcataa aaaacgcggc gaattgcctg ctgcgctacg
     1621 cttatcaggc ctacaaagtt tctgaattac aaacctttgt aggccgaatc aggcgttcat
     1681 gccgcatctg acatgaacaa aacgcacata gtcgcgatta atcgcggaag tttttgaact
     1741 ggaacggctg accgagatcg ccaccacgta ccatcgccat gacagcctgc aaatcatcac
     1801 gagatttgcc cgttacgcgg atttcatcgc cctgaatttg cgcctgcact ttcagtttgc
     1861 tgtctttgat catcttgacg attttcttct gggtcgcgct ctcaatgccc tgtttcagtt
     1921 tcgcttccac aaaccaggtt ttaccgctat gaacgatatt ctccggtaca tccagcgaac
     1981 tgccttcaat gccgcgcttc agcagcttgg cacgcagaat atccagcaac tgattgacct
     2041 ggaaatcgga ctcgctcaac actttgatgg ttttgctggc gtcgttcagc tcaaatgagg
     2101 cttcaacgtt acggaagtca aaacgggact ccacttcgcg gctcgcgtta tcgaccgcgt
     2161 tacgagcttc ctgaagatca acttcagaga caatatcgaa agatggcatc ttttcctctc
     2221 ccttcatttt tgatgcgaag cataataccc gcaaagttaa ataaccgaca gtcctgcacc
     2281 aggacgtatc ccttaattgt gcatagtcgc aacagcatgc acaattaagg gatagcctta
     2341 ttgaacaccc ggagtggttg cgggtgagga ggaacaatga aaattaccgt attgggatgc
     2401 ggtgccttag ggcaattatg gcttacagca ctttgcaaac agggtcatga agttcagggc
     2461 tggctgcgcg taccgcaacc ttattgtagc gtgaatctgg ttgagacaga tggttcgata
     2521 tttaacgaat cgctgaccgc caacgctccc gattttcttg ccaccagcga tctgctcctg
     2581 gtgacgctga aagcatggca ggtttacgat gccgtcaaaa gcctcgcgtc cacactgcct
     2641 gtaaccacgc caatactgtt aattcacaac ggcatgggca ccatcgaaga gttgcaaaac
     2701 attcagcagc cattactgat gggcaccacc acccatgccg cccgccgcga cggcaatgtc
     2761 attattcatg tggcaaacgg aatcacgcat attggtccgg cacggcaaca ggatggcgat
     2821 tacagttatc tggcggatat tttgcaaacc gtgttgcctg acgttgcctg gcataacaat
     2881 attcgcgccg agctgtggcg caagctggcc gtcaactgtg tgattaatcc actgaccgcc
     2941 atctggaatt gcccgaatgg tgaattacgt catcatccgc aagaaattat gcagatatgc
     3001 gaagaagtcg cggcagtgat cgagcgcgaa gggcatcata cttcagcaga agatttgcgt
     3061 gattacgtga tgcaggtgat tgatgccaca gcggaaaata tctcgtcgat gttgcaggat
     3121 atccgcgcgc tgcgccacac cgaaatcgac tatatcaatg gttttctctt acgccgcgcc
     3181 cgcgcgcatg ggattgccgt accggaaaac acccgcctgt ttgaaatggt aaaaagaaag
     3241 gagagtgaat atgagcgcat cggcactggt ttgcctcgcc cctggtagtg aagagactga
     3301 agccgtcacc actatcgatc tgctggttcg cggcggtatc aaagtcacca ctgccagcgt
     3361 cgccagcgat ggcaacctgg ccattacctg ctcgcgcggc gtgaagctgc tggcggatgc
     3421 gccgctggtc gaggtggctg atggcgaata tgacgtgatc gtactgcctg gtggcattaa
     3481 aggcgcagag tgttttcgcg atagtaccct gctggttgaa accgttaaac agttccaccg
     3541 ttccgggcgt atcgtcgcgg ctatttgcgc cgcgccagcc accgtgctgg tgccgcacga
     3601 tatcttcccg attggtaata tgaccggctt cccgacgctg aaagacaaaa ttcccgctga
     3661 acaatggcag gacaagcgcg tcgtctggga tgcacgggta aaattgctga ccagccaggg
     3721 gccggggaca gctatcgact ttggtctgaa aattatcgac ctgctggttg ggcgcgagaa
     3781 agcccatgaa gtggcatcac aactggtgat ggcggcaggg atttataatt attacgagta
     3841 gtgtcggatg cggtaagcgt cgcatctgac cagatgcgac gttacaacca caaattacgg
     3901 gcgatatacc ttcacattgt taaagccctg ctcgcgcaga tagagcgcct gcagacggct
     3961 catcaccccg cgctcacacc acagtagcca ggttctgttc tggtcgagat cgccaaactt
     4021 ggtgctcagt ttatagaacg gcagagaaac tacatcaatc ccttcgactt tcagtggctt
     4081 atcttcctgt tcatcgatag aacggatatc gaggatcacg tcgttcgggc cgaagccatt
     4141 gacggtttcc acttccacca cttcctgctc ggtctgctgg gcgatttcgc ggatatcaac
     4201 gttattcgct tcttcaacca ctttatcgag aatgctgaag tcgaatttct cttcttccgc
     4261 ttcaatcttc gacttaaccg ctttcaccgt cgggcttttg gagataacac cgcaatattc
     4321 cggcatcgtg cgagcaaagt cttcggtgcc aatctggcga gccaggttga tgatgtgctc
     4381 tttgtcgtaa gaaatcagcg gacgcaggat cagcgtatcg gagacgttat caatcaggcg
     4441 caggttggtc agcgtctgac tggacacctg gccgagcgct tcgccggtga ccagcgcctg
     4501 tacgccgtaa cgttcagcca ctttagacgc ggcacgcacc atcatacgtt tgaggataac
     4561 gcccatctga ccgtcgtcga ttttctcgag aatttccccg acgaccggtt cgaaattaat
     4621 agcgacaaaa cgcacgcggt gggagctgcc aaaacggttc cacagataat gcgccacctg
     4681 acgcacgcca atttcatgcg ccgcgccgcc aaggttaaag aaacagtaat gcacgcggca
     4741 gccgcgacgc atcaacatat aactggaaac accggagtcg aaaccaccgg aaatgagcga
     4801 cagcacatct tcctgggtgc cgatcgggaa accgccaata ccttcgtagc ggcctttaat
     4861 cagcaggaga cgatcgtctt ccacttccag atggacagtc acttccggat tggtcagctt
     4921 cacgcgcgcg gactcaatat gctgatttaa accgccgccg acgtaacgtt ccacatcaat
     4981 cgagctaaaa tcatgtttgc cacggcgctt cacgcgtacg cagaaggttt tgccttccag
     5041 ctgatcgcga tactgaacca acgctttctc aaaaatatcg tgcatgtcgg taaacggcac
     5101 gtcttcgact tcgagaatat ggtggatacc cggaatacgg gtcagggcgt cgcgaatagc
     5161 cagacgctgg ttttcatctt ttgcgcgaac ttcgatgtta tcccagtggc ggacgacagc
     5221 gagcgtctca tcatagtgct ttaaaacgtt acgaatgttc ccggtaagga tttttataaa
     5281 gcgcaagcgc acagattggc ttttgatggt gatttccggg aacaatttaa tgataaactt
     5341 catggcggca atggttcgtt ggcaagcctt aagcgacttg tatagggaaa aatacagcag
     5401 cccacacctg cggctgcatc caggcgcgga agtataccac taacatcgct ttgctgcgca
     5461 catcacctta ccattgcgcg ttatttgcta tttgccctga gtccgttacc atgacggggc
     5521 gaaaaatatt gagagtcaga cattcattat gccgaagaaa aatgaggctc ccgccagctt
     5581 tgaaaaggcg ctgagcgagc tggaacagat tgtaacccgt ctggaaagtg gcgacctgcc
     5641 gctggaagag gcgctgaacg agttcgaacg cggcgtgcag ctggcacgtc aggggcaggc
     5701 caaattacaa caagccgaac agcgcgtaca aattctgctg tctgacaatg aagacgcctc
     5761 tctaacccct tttacaccgg acaatgagta atggactttc cgcagcaact cgaagcctgc
     5821 gttaagcagg ccaaccaggc gctgagccgt tttatcgccc cactgccctt tcagaacact
     5881 cccgtggtcg aaaccatgca gtatggcgca ttattaggtg gtaagcgtct gcgacccttc
     5941 ctggtttatg ccaccggtca tatgtttggc gttagcacaa acacgctgga cgcacccgct
     6001 gccgccgtag agtgtatcca cgcttactca ttaattcatg atgatttacc ggcgatggat
     6061 gatgacgatc tgcgtcgcgg tttgccgacc tgccatgtga agtttggcga agcaaacgcg
     6121 attctcgctg gcgacgcttt acaaacgctg gcgttctcga ttctaagcga tgccaatatg
     6181 ccggaagtgt cggatcgcga cagaatttcg atgatttctg aactggcgag cgccagcggt
     6241 attgccggaa tgtgcggtgg tcaggcatta gatttagacg cggaaggcaa acacgtacct
     6301 ctggacgcgc ttgagcgtat tcatcgtcat aaaaccggcg cattgattcg cgccgccgtt
     6361 cgccttggtg cattaagcgc cggagataaa gggcgtcgtg ctctgccagt actcgacaag
     6421 tacgcagaga gcatcggcct tgccttccag gttcaggatg acatcctgga tgtggtagga
     6481 gatactgcaa cgttgggaaa acgccagggt gccgaccagc aacttggtaa aagtacctac
     6541 cctgcacttc tgggtcttga gcaagcccgg aagaaagccc gggatctgat cgacgatgcc
     6601 cgtcagtcgc tgaaacaact ggctgaacaa tcactcgata cctcggcact ggaagcgcta
     6661 gcggactaca tcatccagcg taataaataa acaataagta ttaataggcc cctgatgagt
     6721 tttgatattg ccaaataccc gaccctggca ctggtcgact ccacccagga gttacgactg
     6781 ttgccgaaag agagtttacc gaaactctgc gacgaactgc gccgctattt actcgacagc
     6841 gtgagccgtt ccagcgggca cttcgcctcc gggctgggca cggtcgaact gaccgtggcg
     6901 ctgcactatg tctacaacac cccgtttgac caattgattt gggatgtggg gcatcaggct
     6961 tatccgcata aaattttgac cggacgccgc gacaaaatcg gcaccatccg tcagaaaggc
     7021 ggcctgcacc cgttcccgtg gcgcggcgaa agcgaatatg acgtattaag cgtcgggcat
     7081 tcatcaacct ccatcagtgc cggaattggt attgcggttg ctgccgagaa agaaggcaaa
     7141 aatcgccgta ccgtctgtgt aatcggcgat ggcgcgatta ccgcaggcat ggcgtttgaa
     7201 gcgatgaatc acgcgggcga tatccgtcct gatatgctgg tggtcctcaa cgacaacgaa
     7261 atgtcgattt ccgaaaatgt cggcgcgctc aacaaccatc tggcgcagct gctttccggt
     7321 aagctttact cttcactgcg cgaaggcggg aaaaaagttt tctctggcgt tccgccaatt
     7381 aaagagctgc tcaaacgtac cgaagaacat attaaaggca tggtagtgcc tggcacgttg
     7441 tttgaagagc tgggctttaa ctacatcggc ccggttgacg gtcacgatgt gctggggctt
     7501 atcaccacgc tgaagaacat gcgcgacctg aaaggcccgc agttcctgca tatcatgacc
     7561 aaaaaaggtc gtggttatga accggcagaa aaagacccca tcactttcca cgccgtgcct
     7621 aaatttgatc cctccagcgg ttgtttgccg aaaagtagcg gcggtttgcc gagctattca
     7681 aaaatctttg gcgactggtt gtgcgaaacg gcagcgaaag acaacaagct gatggcgatt
     7741 actccggcga tgcgtgaagg ttccggcatg gtcgagtttt cacgtaaatt cccggatcgt
     7801 tacttcgacg tggcaatcgc cgagcaacac gcggtgacct ttgccgccgg tctggcgatt
     7861 ggtgggtaca aacccattgt cgcgatttac tccactttcc tgcaacgcgc ctatgatcag
     7921 gtgctgcatg acgtggcgat tcaaaagctt ccggtcctgt tcgccatcga ccgcgcgggc
     7981 attgttggtg ctgacggtca aacccatcag ggcgcttttg acctctctta cctgcgctgc
     8041 ataccggaaa tggtcattat gaccccgagc gatgaaaacg aatgtcgcca gatgctctat
     8101 accggctatc actataacga cggcccgtcc gcggtgcgct acccgcgcgg taacgcggtt
     8161 ggcgtggaac tgacgccgct ggaaaaactg ccaattggca aaggcattgt gaagcgtcgt
     8221 ggcgagaaac tggcgatcct taactttggt acgctgatgc cagacgcggc gaaagtcgct
     8281 gaatcgctga acgctacgct ggtcgatatg cgttttgtga aaccgcttga tgaagcgtta
     8341 attctggaaa tggccgccag ccatgaagcg ctggtcaccg tagaagaaaa cgccattatg
     8401 ggcggcgcag gcagcggcgt gaacgaagtg ctaatggccc atcgtaaacc agtacccgtg
     8461 ctgaacattg gcctgcctga cttctttatt ccacaaggaa ctcaggaaga aatgcgcgcc
     8521 gaactcggcc tcgatgccgc cggtatggaa gccaaaatca aggcctggct ggcataatcc
     8581 ctactccact cctgctatgc ttaagaaatt attcatagac tctaaataat tcgagttgca
     8641 ggaaggcggc aaacgagtga agccccagga gcttacataa gtaagtgact ggggtgaacg
     8701 aatgcagccg cagcacatgc aacttgaagt atgacgagta tagcgggagt ggcagcatgc
     8761 aatacaaccc cttaggaaaa accgaccttc gcgtttcccg actttgcctc ggctgtatga
     8821 cctttggcga gccagatcgc ggtaatcacg catggacact gccggaagag agcagccgtc
     8881 ccatcattaa acgcgcgctg gaaggcggca taaatttctt tgataccgcc aatagctatt
     8941 ccgatggcag cagcgaagag atcgttggtc gcgcactgcg ggatttcgcc cgtcgtgaag
     9001 acgtggtcgt tgcgacgaaa gtgttccatc gcgttggtga tttaccggaa ggattatccc
     9061 gtgcacaaat tttgcgctct atcgacgaca gcctgcgccg tctcggcatg gattatgtcg
     9121 atatcctgca aattcatcgc tgggattaca acacgccgat cgaagagacg ctggaagccc
     9181 tgaacgacgt ggtaaaagcc gggaaagcgc gttatatcgg cgcgtcatcc atgcacgctt
     9241 cgcagtttgc tcaggccctg aagctacaaa aacagcacgg ctgggcgcag tttgtcagta
     9301 tgcaggatca ctacaatctg atttatcgcg aagaagagcg cgagatgctg ccactgtgtt
     9361 atcaggaggg cgtggcggtg attccgtgga gcccgctggc gcgggggcga ctgacgcgtc
     9421 cgtggggaga aactaccgca cgactggtgt ctgatgaggt ggggaaaaat ctctataaag
     9481 aaagcgatga aaatgacgcg cagatcgcag agcggttaac aggcgtcagc gaagaactcg
     9541 gtgcaacacg agcacaagtt gcgctggcct ggttgttgag taaaccgggc attgccgcac
     9601 cgattatcgg aacttcgcgc gaagaacagc ttgatgagct attgaacgcg gtggatatca
     9661 ctttgaagcc ggaacagatt gccgaactgg aaacgccgta taaaccgcat gcggtcgtag
     9721 gatttaaata agagtccagg cctgatgaga cgtgacaagc gtcacatcag gcatcggtgc
     9781 acaactacga cagaataccc agcggccagt gatgaccgat aaagtacagg atgcctgcgg
     9841 aaatcacccc ggcgacaata tcgtcgatca tgatccccat gccgccatgt acattgcgat
     9901 caaaccagcg gatcggccac ggcttccaca tatccagaat acggaaaatc acaaacccgg
     9961 cggcaaccca ctgccagtca ttggtcggca gcgccatgag cgtgatccac ataccaataa
    10021 attcgtccca gacaatgctg ccgtgatcgt gcacgcccat atctttcgcc gtttgatgac
    10081 aaagatagac gccgatacag atccccaaca tcaccaccag cgagtagagc tgccagggta
    10141 aaaaggtcat cagataccag aacggaatcg ctgccagcga tcccatcgtg ccaggaacga
    10201 ttgagcttaa tccacttccg aatccgacag caagcagatg ccacggatta ctcatcttca
    10261 ggcgactttt cgcgacatct ttatggcgtg gcaaaatggt catatccttt ccagtcaaac
    10321 gtgacaggtt cgccgtcacg aataaaacaa agcccttcga tatcggcggt catttgcccg
    10381 atacaggtaa acggtacgcc caggtgtccg agagccacat ccagcgcgcc acggttcagt
    10441 tccggcacag tgaaacacaa ctcgtaatct tcaccgccag agagcgccca gcgcagcgcc
    10501 tgttccggtt caacatggcg agaaagcgca tcagaaaacg gcagcaatgc caggtcaata
    10561 cgtgcgccgc agtcgctggc tttcacgata tgcccgagat cggaaatcag accgtcagag
    10621 agatcgatag ctgaatttgc cagatcgcgc agtgcctgcc cctgtaaaat acgcggcgat
    10681 ggacggagat gacgtttgat caagtagtca gcatctttag catcggcaac ctgcaaacgg
    10741 ttttgcaaaa tcgccagccc ggcggcgcta tcgcccggtg taccggtcac atagatccag
    10801 tcacccggtt tcgccccaga gcgcgttaag gctcgtccca tcggaacaaa gccgtggata
    10861 cccaacgtca ttgataatgg cccacgcgtg gtatcgccgc caatgagttg catatcgtaa
    10921 taattgagaa gatcaaacaa actgtcgctg aaggactcaa gccacgcttc gtctacgtcc
    10981 ggtaaggtta atgccagcgt cagccaggcc ggatcggccc ccatcgctgc cagatcgctt
    11041 aggttcactg ccagtgcttt atacgccaga tcagcaggat cgatatcatg gaggaagtgg
    11101 ttgcccgcca ccagcgtatc agtgctgatc gccagggtct gtttctcggg gatattgaga
    11161 agagcgcaat cgtcgccgat gcccagttcg acatcaagac gagaacttct tacacggtca
    11221 aaataacggg caatcaggga gaactcgcca catgccatac gttatgcctc agcaggtaaa
    11281 aaagaaaagg ccggcgatcg cggaacggtc ttccgtgaat ctaccggcct ggatatcact
    11341 ttttgttagg gcgaatcaca ggtgctgctt tatcgagtac gccgttgacg aacttatggc
    11401 tgtcttctgc accgaacgat ttcgccagtt cgatcgcttc gttaatggcc actttgtatg
    11461 gcacatcgct acgtttagac agctcgtaca gcgcaatgcg cagtactgct ttttctacct
    11521 gtcccagttc ttccagcagg cgggacaggt atggcttcat cagtccgtcg aggtatgcgg
    11581 tgttagtcgc caccccggcc agcagctcac ggaagtacag gacgtcaacg tcttttacat
    11641 cctgttcagc caggaactgg tattcaacat cagcgatgtc gttctgggac aactgccagg
    11701 agtagagcgc ctggacggca cactcacgag cgcggcgacg agcagcaggt ttcacggatt
    11761 tccccttact aatttcaggc cttgatggct ttcaatacat taatcatttc aagcgcggtc
    11821 agtgcagctt ctgcaccttt gttgccagct ttggtgccag cacgttcgat cgcttgttca
    11881 atgctttcag tggtcagaac cccaaaagca accggaattt cgctgtcctg ggcaacatgc
    11941 gccaggccgt tgcttgcacc accagcgaca tattcaaagt gggcagtgcc accacgaata
    12001 accgtaccca gcgcaatcac cgcgtcgtat ttaccggttt tagccagtgc acccgccgcc
    12061 agcggcagct cataggcacc aggcacccaa acaacggtaa tgttttcatc ttttacctga
    12121 ccgatacgtt tcagtgcgtc aattgcacct tccagcaggc tgtcattgat aaagttgttg
    12181 aaacgcgcga tggtgatggc gacgcgagcg tccggggtag caacgttagc ttcaataatg
    12241 ttcattttct tccttcgggt tcgagtatgg ccccgcaggg gggcggattt tagcataata
    12301 tttcgtgcgc tgcttccctt tcgagccggg agatcatgca cccactaaat gcaggcaaac
    12361 atccgggcct acatgacgta tctctttgaa tttaaattgg ggggcgtcgg ctaatttctc
    12421 aagccctggc agcgtgcata atccgcgggc gtcgctgcct aatagtttag gtgcgatata
    12481 gacaatcagc tcatcgacta aacccgcctg cagcaatgcg ccagcgagcg ttggccccgc
    12541 ttccacccag atactgttaa tttgctgttt acccagttgc atcatcagta caaccagatc
    12601 cagatgacct ttatgctctg gaatcagcaa ggtacgcacc gtttccggcc actcactaga
    12661 atcttcctgc gtacgcgcga accaggtttc gccgggctgc tgcacaatgc gatgttccgg
    12721 cgtcacgcga ttttggctat caatcacaat acgtactggc tggcggagat tttgttgcgg
    12781 gtagagcgcc tgagtttgtt catccagttc agaccaacgc actgttaagg ctggatcatc
    12841 cgccagcacc gtggcgctgc tggttaaaat ggcatgactt tgtgcgcgct gtcgttgtac
    12901 gtcgcgccgc gcctgaggcg aagtgatcca ctggctttcg ccgctcgcca tcgccgtgcg
    12961 gccatcaagc gatgcgccaa gttttaactg aatataagga aagccggtgc gcatccgctt
    13021 gagaaagcct ttattcaatt gctcggcttc actcatcatc agcccgtggc tgacgtcaat
    13081 gccagcctgt tgcagacggt aaagtccacg cccagcgacc tgcgggttag gatcttgcat
    13141 cgaggcaacc acgcgcgcta cgccagcggc gatgagtgcg tcacagcacg gtggcgtacg
    13201 accatgatgg ctacagggtt cgagtgtgac ataggcggtc gcacctttgg ctttttcacc
    13261 cgccatacgc aacgcgtgta cttcggcatg tggttcaccc gcacgttggt ggtaaccttc
    13321 accgacaatt tcgccatctt tgacaatgac gcacccgaca ttcgggttgg gatgcgtggt
    13381 aaaacgtcct cgttgcgcca gctttagcgc tcgcgccatg taatactcgt cctgcacggc
    13441 ttagtcctcc aggcgcgcga tctcttcgcc aaattctttg atatcttcga aactgcggta
    13501 gacagaggca aaacggatat aggcgacttt atcgagcttt ttcaattgct ccatcaccag
    13561 attgccaatc atcttgctcg gcacttcgcg ctcaccggtg gcgcgcagct gcgatttaat
    13621 atgattgatt gccatttcga cgtcatcgga actcaccgga cgtttttcca gcgcccgcag
    13681 cattccgcta cgcaatttct cttcattaaa cggttcacgc acgtcgttgc tttttacaac
    13741 acgcggcata accagctccg ccacttcaaa ggtggtgaaa cgttcattac acaccagaca
    13801 ctgccgacgg cggcgtacgg atgaaccctc gcccacgaga cgagagtcaa ttaccttagt
    13861 gtccacggcg aaacagaatg ggcaatgcat acggcgtcct gactaggtgg ttaacagaaa
    13921 tctattttac cctgaactga cgatacaaca aaggcgcaac gcttttgaga taaaggatcg
    13981 atgctttcgc tgcttttacc ctctaccatt aggctaattg cgacgagaaa gggacgacct
    14041 atgaatacta acgtttttcg actgctcctg ctgggaagcc tgttcagcct tagcgcctgt
    14101 gtgcagcaga gcgaagtgcg acagatgaaa cacagcgtca gcacgctgaa ccaagagatg
    14161 acgcaactca ataaagaaac ggtcaaaatc actcagcaaa acaggctgaa tgcaaaatcc
    14221 agcagtgggg tttaccttct gccaggagcg aaaacaccgg caagactgga aagccagatc
    14281 ggtactttac gtatgtcgct ggtgaatatt acgcctgatg cagatggcac caccctgacg
    14341 ctacgtattc agggcgagtc gaatgatccc ttgcccgcat tcagcggaac tgttgaatat
    14401 gggcaaattc agggaacaat agacaacttt caggaaatca atgtgcagaa tcaattgatt
    14461 aatgcccctg ccagcgtcct cgcccccagc gatgttgata ttccgttaca gttaaagggt
    14521 atttctgtag aacagttaga cttcgtacgt atccacgata ttcaacctgt tatgcagtaa
    14581 acgtatttcg gggcgatttt gtgcgtctcg caacatcttt ccccgtcatt ttgttactcc
    14641 gcttacatca ccctgattga tagtaaaagt ttgcaacaag ggtgaaagtc agtacaatcc
    14701 ctgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga actgtgaaac
    14761 gaaacatatt tttgtgagca atgattttta taataggctc ctctgtatac gaaatattta
    14821 gaaacgcaat ttgcgccttt ttcactcccg caagggattt tcaaacagtg gcatacatat
    14881 gaaaaaaaca ttactggcag ccggtgcggt actggcgctc tcttcgtctt ttactgtcaa
    14941 cgcagctgaa aacgacaaac cgcagtatct ttccgactgg tggcaccaga gcgttaacgt
    15001 tgtcggaagc tatcacaccc gtttcggacc gcagatccgc aacgatacct accttgagta
    15061 cgaagcattc gctaaaaaag actggttcga cttctatggt tatgcggatg cgccggtatt
    15121 cttcggcggt aactccgatg ctaaaggtat ctggaaccac ggttctccgc tgtttatgga
    15181 aatcgaacca cgtttctcca tcgacaagct gaccaatact gaccttagct tcggtccgtt
    15241 caaagagtgg tacttcgcga acaactacat ttacgacatg ggtcgtaata aagatggtcg
    15301 ccagagcacc tggtacatgg gtctgggtac cgacatcgat actggtctgc cgatgagcct
    15361 gtccatgaac gtctatgcga aataccagtg gcagaactat ggcgcagcaa acgaaaacga
    15421 gtgggacggt taccgtttca aaattaaata ctttgtgccg attaccgatc tgtggggcgg
    15481 tcagctgagc tacatcggct tcaccaactt cgactggggt tccgatttag gggatgacag
    15541 cggtaacgca atcaacggta ttaagacccg tactaataac tctatcgctt ccagccatat
    15601 tctggctctg aactacgatc actggcacta ctctgtcgta gctcgttact ggcacgacgg
    15661 tggtcagtgg aacgacgatg cagaactgaa cttcggcaac ggcaacttca acgttcgctc
    15721 taccggctgg ggtggttacc tggttgtagg ttacaacttc tgattatgaa aatgccggga
    15781 tttattcccg gcatttcaga ttgttaacaa cgtgcgcttt gttcatgccg gatgcggcgt
    15841 aaacgctttg tccgacccac aaaagcacgt aaattcaata tattgctgaa ataacgtagt
    15901 cctgataagc gtagcgcatc aggcaatttt acgttttaat cacttcttct tgttcatcat
    15961 cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg acatctttct gcgcatcttc
    16021 ccctgcgata acggtcgtac catactgatc cgcttcggta tatttcgaat gctcatgatc
    16081 gtggcaatag agacaaagca attcccagtt actgccatct tccgggttat tggtatggtc
    16141 gtgatcaatg tggtgaacag taagttcacg caggttggaa taaacaaact cgcgggaaca
    16201 gcgaccgcag acccacagat atatttttaa tgctttttcg cgatagccgc tttctaaccg
    16261 cgcatagttt tttgggatga tagccatgaa gagttacctg gatgaaatgt agggttacgc
    16321 gcagtttccc aagatgcggg gattttcgca atctccatac atcaaaaaaa atcccgatct
    16381 tctgaccggg attttcaaca tcaacgggaa cttgattacg gcagaattga cggctgatcc
    16441 gccccttctt tttccacttt ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga
    16501 gccaacgcag atgccacata gatggaagat gcagtaccga tggaaacacc gataagcatg
    16561 gtcagcgaga agccttccag taccggacca ccgaagaggt acagcatcag gataaccatc
    16621 aaggtagtac cggatgtgat caaggtacgg tgcagcgtct gggtcaagga cacgttaaag
    16681 atttcgtaag gcgtaccgcg acggatcttg cggaagtttt cacgaatacg gtccgatacc
    16741 acgatactgt cgttaagcga gtaaccgata accgacatca acgatgccac aatggtcagg
    16801 tcaatctcga tatggaataa cgacaaaata cccagcgtaa tgataacgtc gtgcgccagc
    16861 gcaataacca cccctgccgc cagtcgccac tcaaagcgaa aacctacgta cacaaggata
    16921 gacagcagcg ccgccatcaa cgccatcgca ccggtttgcg caaggtctgc ccccacgctc
    16981 ggaccgacga actcaatacg cttcactgct gcattctgat tggtggattc gttaatcacc
    17041 ttcagaacct ggctgcccag cacctgaccg ccggtttcgc cttcagcagg cggcatacgg
    17101 accatgatgt cgtggctgct accaaagttt tgcagcattg gctcttcaaa accggctttt
    17161 tgcaatgcat cacgcattac gtcaatttca gccggttttt cgagcgtaat ttcgataacc
    17221 gtaccaccgg tgaaatccag cccccagtta aagccgcgca cgcccataat aacgatagcg
    17281 gcgattaaca gcagaccaga gatgccgaaa gcccagtagt cccagcgcat aaagtcatag
    17341 actttacggc cgtggtttag ttgttcaaca gtatattcct gtgccacatc gcactcctca
    17401 gattgacagc ttcttgacgc gcttgccgcc atatagcagg tttacgatgg cacgcgtacc
    17461 gacaatcgcg gtaaacatcg acgtcgccac accgataccg gtagtaatcg cgaacccttt
    17521 aattgccccg gtacccactg cgtacaggat gatgacttta atcagcgtgg tgatgttcgc
    17581 atcgaagata gaactgaatg cgccacgata accttcatca attgcctgtt gaacagtacg
    17641 tccgttgctc aactcttctt taatacgttc gttgatcagt acgttcgcat cgaccgccac
    17701 cgcaagggtt aagacgatac ccgcgatacc tggcatactc agcgttgcgc ctggcaacag
    17761 cgacataatg ccgacgatta agatcaagtt ggcaatcaga gcactggtcg caatcagacc
    17821 aaacttctta tagaagatga tcatgaacag aatagaaacc agcagaccgg caaggcaagc
    17881 ctccagcccc tgttcaatgt tctgcatacc cagggttggg ccaatggtac gttcttcaac
    17941 aatctgaatc ggcgcgatca acgcaccggc acgcagcagc agtgacagct gacgggcttc
    18001 gttcgggttg ttgatgccgg tgatacggaa gctgttaccc agacgagact ggatgttggc
    18061 gatgttaatc acctcttcct gtttcaccag aaccgcacga ccatttgcat ctttcttacc
    18121 gctgtctttg tactccacaa acagggttgc catcggtttg ccgatgttgt ccttagtgaa
    18181 gttagacatg atgttaccac cagcgctatc gagcgagatg ttaacctgcg gttggttgta
    18241 ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg gtcagaatta cgcgtttgta
    18301 cagcacaact ggctgacctt cgcgggtctg tttcacttca gagtcgcccg gtacgcgacc
    18361 agatgccgca gcggcctggt caacgttggt gtttaccaga cggaattcca gcgttgccgt
    18421 cgcacccaga atctctttcg cacgcgcagt gtcctgaata cctggcagtt caacaacgat
    18481 acggtcagca ccctgacgct gaaccaccgg ctccgccacg ccaagttggt ttacacggtt
    18541 acgcaggata ttgatgttct gctgcaccgc atattcacgc gcttcactca gacgagcatc
    18601 gctcattacc gcacgcagct ggttgctgcc ctggctacta atcaccaggt ccggatggcg
    18661 cttgctcaga tacgcaatgg cttcatcacg agctttagca tcgcggaaag taatgctcag
    18721 gccgtagttg ttttctttac gaacggtggt atacgggatg cccttttcac gcagatcgct
    18781 gcgcaggcta tcgatatttt gttcctggag tttgccaagc gcggtgtcca tatccacttc
    18841 catcaggaag tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct cagcgtgaat
    18901 agctgccagc cagcgcggcg ttgccggggc aaggttaagc gccacgacgt atttgtcacc
    18961 cataacgccc attaatgctt cacgagcgcg cagctgggtg tcagtggagt cgaagcgcgc
    19021 aagaatagcg ccctcttcca gtgccacaga cttagcagtt attttttctt cttgtaacgt
    19081 tttctggacc tggatcagcg tttgctcact ggcggcgact ccgcgcgcac cagtgatctg
    19141 aacagccgga tcctcaccaa acaggttggg aagcgcatac agcagaccga tgacaatcac
    19201 cacgatcagc atgacgtact tccacaaagg ataacggttt aacacggcaa ttcccttagg
    19261 gaaaaatttt aattacagcg ccttcatggt gcctttcggc aggacggcag ctacgaagtc
    19321 acgtttaata actacttcag tggtgtcatt cagcgcgata gcaatgtagc cgttttccgc
    19381 tactttggtt acgcgaccaa ccaggccacc gttcgtcaga acttcatcac ctttggcaat
    19441 ggagtccatc agctttttgt gttctttggt gcgcttctgc tgtggacgca ggatcatgaa
    19501 atagaaaatc agaccgaata ccaccagcat caaaatcaaa gacatcgggc taccttgcgc
    19561 cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag ctcattaaat ttccctcatt
    19621 attaatatta atcaacgttc aaaggtggta cttctcgccc ctgacgctgg taaaaatcag
    19681 ttacgaagct ctctaattta ccctcttcaa tagccttgcg taaacccgcc atcaaacgct
    19741 ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt
    19801 caagatgatg caagtaagcg cgtgaataat tgcgacaggt gtagcaatca cactcaggat
    19861 cgagtgggcc agtatcgctc ttatacttcg cattgcggat tttcaccacg ccatcggtca
    19921 cgaacaaatg accatttcgg gcgttgcggg ttggcattac gcagtcaaac atatcgatac
    19981 cacgacgtac gccttcaacc aggtcttctg gtttaccaac gcccatcagg taacgcggtt
    20041 tgtctgccgg aatttgcggg catacatgct ccagaatgcg gtgcatatct gctttcggct
    20101 cacccacagc cagaccgccg acagcgtagc catcaaaacc gatatctacc agacctttaa
    20161 cagaaatatc acgtaaatct tcgtaaacgc tgccctgaat gataccaaac agcgcatttt
    20221 tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga gacatctcca
    20281 tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt acactcatca aagatcatga
    20341 cgatatccga accaagatcg tactgaatct ccattgattt ttcaggatcg aggaaaatcg
    20401 gatcgccgtt gatcgggtta cggaagtgca cgccctgttc ggtgatttta cgaatatcgc
    20461 caaggctgaa gacctggaag ccgccggagt cggtgaggat cggccccttc cactgcataa
    20521 aatcgtgcag atcgccgtgc agtttcatga tttcctggcc cgggcgcagc cacaggtgga
    20581 aggtgttgcc gaggataatt tgcgcgccag tggcttcaac ttcttccggc gtcatccctt
    20641 ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt ttccactacg ccacgatcaa
    20701 agaccaggcg gccacgacgt gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt
    20761 ctccactacg tcagaaaaac agtccaacgt tttaaaccag cgccgcggaa ttactccccg
    20821 acgcgctcat taattgcctg cggattgtac gtgataaaca tcgcatcacc gtaactaaaa
    20881 aagcgatatt tctcttctac cgctgcttta taggcgttca tggtgtgttg ataaccggca
    20941 aaggccgaaa ccagcataat cagcgtcgat tccggcaggt ggaagtttgt caccagcgca
    21001 tcgaccactt tgtactggaa gcccggatag ataaagattt gggtatcgtc aaagaacggt
    21061 tcgataagat cgtttttcgc cgcctgagcc gcgctttcca gcgaacgtac tgaggtggtg
    21121 ccaaccgcaa tcacccggtt accgcgcgct ttcgccgcca gtaccgcgtc taccacatcc
    21181 tgcggcactt cagcgtattc cgagtgtatg atgtgatctt caatggtgtc gacgcgcacc
    21241 ggctggaagg tgcccgcacc aacgtgcaac gtcacaaacg ccatctccac gcctttggcg
    21301 cgtaattttt ccagcaaagg ttcgtcaaaa tgcagacctg cggtcggggc tgcaaccgcg
    21361 cccggttttt cgctataaac ggtttgataa agttcgcggt cagcgtcttc gtccggacgg
    21421 tcgatatacg gcggcagcgg catatggccg atgctgttga gaatatccag caccgagcgt
    21481 tcatcattaa attcgacttc aaacagtgcg ccgtggcgcg cggtcattgt tgcgttaata
    21541 ctttcgtcat cgcccagcag cagttctgcg ccaggttttg gcgctttcga ggcgcgaata
    21601 tgcgcaagaa tgcgtttgtc gtcgagcatc cgttcaacca gcacttcaat cttgccgccg
    21661 ctggctttac gcccaaacag gcgcgccggg atcacgcggg tattattaaa aaccagaaga
    21721 tcgccggggt tgagcttatc aagtaaatcg gtgaaagtac cgtgcgtcag cgcgcccgtc
    21781 ggcccgtcca gcgacagtaa acgacagcta ctgcgttcag gcatgggata gtgggcaatc
    21841 agggattcgg gcaattcaaa ggagaaatcg gtaacgcgca tgacactgac tcagactaaa
    21901 ataagaggcg ggtagtctag tgccggggcg ctctccctgc aacctttacc cccttttgga
    21961 tagaatataa tcgatgaatt ttttagctca cctgcattta gcccatctcg cggaaagctc
    22021 gctttccggc aatttactgg ctgatttcgt acgcggaaat cctgaagaaa gttttccgcc
    22081 cgacgtcgtg gctggcattc atatgcatcg acgtatcgac gtattaaccg acaatctgcc
    22141 ggaagtccgc gaagcacggg aatggtttcg tagtgaaact cgccgcgttg cgcctattac
    22201 gctggatgtc atgtgggatc actttctttc ccgccactgg tcgcagctgt cgccggactt
    22261 tccgctacag gaatttgtct gttatgcccg cgagcaagtg atgacgattt tgccggactc
    22321 accgccacgt tttatcaatc tgaacaatta cttgtggtca gagcagtggc tggtgcgcta
    22381 tcgcgatatg gatttcatcc agaacgtgtt aaacggcatg gcaagccgcc gcccacgtct
    22441 ggatgccctg cgtgactcct ggtacgattt agacgctcat tatgacgccc tcgaaacccg
    22501 cttctggcag ttttatccgc ggatgatggc gcaggcgtca cacaaggcgt tataaaacgt
    22561 tagttcatcc ataccgtagc cgaaatggca ggcaaagcca gaatcccgtc agtcagttgc
    22621 ctatgccctt ctttgcattg ccattgcacg gcattgagca acggtgacgc gggtagcacc
    22681 acttcacaag cttcgccacg gttgattgcc accagtacac gctgctgatt cagcacgcgg
    22741 acaaatacca ccacgttatc ttccgcatac agcacctgac agccgccatg acgtagcgcc
    22801 tgacttttct tacgcagcgc aatcattcgc tggtacagcg cgaataacgc cgtatcctgc
    22861 ttttccacct gccaggggaa cggtttacgg caaaacggat cgtttttgcc atccagtcct
    22921 acttcatcac cgtaataaat gcacggtaca ccaggccagg tgaacagcca gaccaccgcc
    22981 agcggcaggc gcgcaatatc ccgaccgagc agcgttttaa atcgcgcagt atcgtggctg
    23041 tcgagctgat taaacatacg taattgttgt tgatgagaaa gccctgcgcg gtaattatcc
    23101 atccaggcca tacaggtttg ggcatcaatt tgctgcggat cgtaagagat atcggtattg
    23161 gcaagaaatc cccacaacgg gaatgtgaag ccacgatagt tcatggcggc atcttccaca
    23221 tcggcctgta accattgccg tgcatcgcca aaatgttcgc cgacaatata cgcttccggc
    23281 tgggtttctt tcgccgcttc ggtgatccca gcaacgtgct gcatattatt gcgcgcccca
    23341 cccgcctccc ccagcatatg caccacatcc agccgccagc cgtccatact ccacggcgct
    23401 ttcagccaat ggcggacaat actgtcttcc ccgcgataaa tttcattcac cagactttcc
    23461 gactgataat ccagcttcgg caagctggca tagccaagcc agtcgagcgc cgtgccatca
    23521 tcactaaacg agtaccagtc gcgccagggc gattcagggt tgtgacaagc tccccccgtg
    23581 ccacgattgt gcctgtcaaa ccaggcatgg gaatcgccac tgtggttaaa cacgccatcc
    23641 agcaccagcc gcattcccag ctgctgcgta ttgtggcgta agcgcagcaa cgccccatca
    23701 ccgtcaaact gcggatcgac atggcgataa tcctcggtat cgtatttatg tacgctggga
    23761 gctttaaaca ccggattgag atacagcgcc gtcacgccaa gctttttcag atacggcagt
    23821 ttttcgctta tcccgtccag atcgccgcca tagaacgttg atccgcccgc ctgcgccgtg
    23881 accggttcat cccagtcacg caagatgatc tcttgtccgg ctgcatgatg gtaatagaca
    23941 tgatcctgtt cagcttcacg aggaagacta cgcgcaaaac gatcagggaa gatctgataa
    24001 aaaatctgat ccgcagccca ttgtgggccg atatccggta catcgacggc aaactgctcc
    24061 agtcgtgccg gcggcattcg gctgaagccc tgcggtgtaa accaacgctg gcgatcgtgc
    24121 cacagcagtt tgaaactgta acgccgccgg ggttgtccgc tggagagatc aatcgccgca
    24181 cgccatgcgg tgacgccagg ctgctgctga ctgcgctgct tatgcatcga tactgacatt
    24241 tcttcgttat cgtgttctgt acgcagcata atgcgctgcg gtgggtcttc gcccgtcagc
    24301 cacagtgtaa tgagcagttg atctttgctt tgtttaacaa atgggggcac cggcaggtgc
    24361 catgcattta acatcataat tcccctgtga tgaaaatctg atcaccttgc catagcctaa
    24421 tgcaggatca ctcatcatct gaaggatatt tgggggagga ggacgggggc ggataacttt
    24481 cggaatagcc ggatcaggat ttcctcctga accggcttac agaacgcaag tattattggt
    24541 tttcagccag ctgacgatcg tggcggcgtt taaacatcca gccaatcaac agcacaacaa
    24601 tccacgcgaa accgacatac agcgagatac gcgtatccgg gtgataacca atcaacccga
    24661 taataaagag caggaaaatc agcccgccga tggtcgttgc taccccaccc ggcactttaa
    24721 atttcagcgc cttaacttct tctggcggca aacggcgacg gaaggcaatt tgcgacagca
    24781 ggatcataat ccacacccac accgtggcga aggttgccag cgaagcgatc accaggaaga
    24841 cgttttccgg catgatgtag ttcagataca ccgcaaacag cagcgcggta gtcatcacca
    24901 gcaccgtaac ccacggaata ccgcgacgcg acgttttgct gaaaattttc ggcgcgctgc
    24961 cctgctctgc cataccgtgg agcatacggc ctacgccaaa tacgtcactg ttaattgccg
    25021 acagcgaagc agtcagcaca acaaagttaa gaatgctggc ggcaaaggta atgcccatat
    25081 gctggaacgt cagcacgaac gggctaccgg cagtgccaac ctgattccac gggtagatag
    25141 acataatgac gaacagcgta ccgacgtaga acaccagaat acgcatcggc acggagttaa
    25201 tcgcacgcgg tatcgatttc tcaggatctt tcgcttcacc ggcggtaatc ccgataattt
    25261 cgatcccacc gtaagcaaac atcaccattt gcaacgacat caccatccca agccagccgt
    25321 tactgaagaa gccgccgttg ctccacaggt tatgaatacc ggtcggttgc ccgccgttgc
    25381 caatccccca gatgatgatg ccgaaaccgg cgacaatcat gatgatgatg gtggcgactt
    25441 taaagaacga gaaccagaat tccagctcac cgaagacctt cacgctcatc aggtttacgg
    25501 cgcaaatgat cagcaccacg ctcagtaccc aaatccagtg cggcaccgtc gggaaccaga
    25561 cacccatata gataccaaaa gcggtcacat cggcgatggc gacaataagg atttcaaagc
    25621 aataggtcca gccggtaatg taacctgcca gcgggccgag gttttcctgc gcataacgcg
    25681 agaaagagct ggctgccggg ttatgtaccg acatttcccc cagcgcacgc ataatgatat
    25741 acgccgcgat accaccgata atataggcca acaacacgct cggaccggcc attttgatgg
    25801 cgtctgccga accgtaaaac agcccggtgc caattgctga acccagtgcc ataaagcgta
    25861 tgtggcgggt acttagccca cgctttagct tgttcttact ttccatcaat tcctaaccca
    25921 tcaacacaat aaaaaaccac ggggcatcgc agccccgtgg ttaaaacaaa tgttcagtga
    25981 tttagtgagc gctggaggtc acctgacgac ctgccgcgcg atcccagata atggccagaa
    26041 ccaccatcac cactgttggc attaaccacg ccagaccttg ttcggccagc ggtaaacgct
    26101 gcgcccagga cggtaagata tcgctgaatg cagatgcttt gatcccgtcg agaataccaa
    26161 aaagcaggct gataaacatc ggcggagcaa tcacgcggga cgaattatgc caccatgagc
    26221 gtgtaaaact taatacaacc agtgcgatac acggcggata aatagcggtc agcaccggta
    26281 cggagatctg gatcagctgg cttaagccga ggttagaaac caccatcgag aagccgccga
    26341 ggataaacac cagcgtacga taagagagcg gtacgtactg ggcaaagaat tctgcacaag
    26401 cacaggtcag gccaactgcc gttaccaggc aggcgatgaa gattaacgcc gccagcagga
    26461 agctaccgcc gccgccaaag gtgtgctgaa cgtaagcatg cagaatagca gcgccgtttg
    26521 cagactgatc gaccagcgac gcgctgtctg aacccagacg gaacagcgcc aggtacagca
    26581 gagtcagacc aacacccgcc atcaggccag cccagacggt ataacgggtc agcagacgcg
    26641 cttcggtaac gccacgagaa cgcgccgcgt taacaataac gataccaaac accattgcgc
    26701 ccagcgtatc catggtcaga tagccgttaa cgaagccgtt agaaaacgca gcgttttgat
    26761 aagcctcagt cgccgtgctg atagaacccg ccggccagac aatcgcggca acagacagga
    26821 tgaccagcgc gataattttc agcggcgcaa ggaagttgcc cacggtatcc agcagcttgc
    26881 ccggatagag cgaaaccaga ataacgatag cgaaatagac caggctgtag ataaacagcg
    26941 gcagcgcgga atcacccgtc agcggcgcaa tccccacttc aaaggaaacg gtagctgtac
    27001 gcggcgtagc gaaaagcggc cccaccgcca ggtaacaaac cgttgccagc agtacgccag
    27061 cgactttacc gattggcgtg ctgaggctgt caacaccgcc gccaactttt gccagcgcca
    27121 ctaccgttaa taccggcagg ccaacggcag taatgaggaa gccgaatgcc gcagtccaga
    27181 cgtgttcgcc tgcctgtaag ccgaccattg gagggaaaat aatgttacct gcgccgacga
    27241 acaacgcaaa tgtcataaag cccagagcga tgatatcgcg cgatcttaat tgatgggtca
    27301 taaatcatac tgcctgtgga tgtggtgttg taacgttaaa tttttgtgcc aatccctggg
    27361 gatgatacag cggtatattt aaacaatttt agcgggaaat actcccaacc aggcgcggcg
    27421 aagaatagtt tttcgattta ccgcgttaat gcagtcagta tgacagcacc tgaagcgcaa
    27481 tttaaacgct tataacgttt taaagcaagg agggatctaa aaaccagagg tatatagcga
    27541 taagaaatca ccaaccagta taaaacacta tttccgtgaa aaagacatgg ctaatcatgc
    27601 gaacaaaaaa tcaccaaccg ttgtaaaatc taacgcccgt attttgactg caaagaaaat
    27661 aagccagcat cggctggctt atggcaataa aagatgacaa aggcggatta atcgctgttt
    27721 ttggcaatta aacgttccgg gataacaaaa ctgaaacgtg ttccttttcc tactgtactc
    27781 tcaatattca ggcgactttc gtgatgattc accgcatgtt tcacgatcgc taaccctaat
    27841 ccgctaccgc cggtttgccg ggaacgcgct ttatcaacgc gataaaaacg ctcggtcagg
    27901 cgcggaatat gctccggtgc aatgcccggt ccgttatctt caacgctaaa ttcggcaccg
    27961 tgcggcactc gcagccagcg tacggtgata tgcgtgcctt ccggcgtatg attcacggcg
    28021 ttatagacca ggttcgaaat cgcactgcgt agctgatctt cgttgccaga caccttgagg
    28081 ccgttatcta tctcaaaggt aaatgtctgt tttttctgac tcagagtctg agcctcgcgc
    28141 tcaacaacgc gcagcatcat cggcacatca accttttcat tgagcaattg cgtcggcgcg
    28201 gcttcaattt tcgacagcgt tagcaactgc ttcaccagcc cttccatccg ctgggtctgc
    28261 tcgcgcatgg tgtgcaacgc tttttcgcgt accgcgcctt ccagcggctg ctcatccatc
    28321 atctccaggt aaccctgtaa cacggtcaat ggcgtacgta actcatggct cacgttggca
    28381 aaaaagttac gccgcgcccc ttccagttga tgcatttgcg tgacatcacg cgccaccatc
    28441 agcaactgtt tgtgggtata aggcatgacg cgaatttcca gatgccgccc ggtgttgagc
    28501 accagattga gcgggcgaga aaaatcacgc gttttcagat attgcgtaaa ctccgggtaa
    28561 cgcagcaggt taaggatgtt ctgcccgtta tcttccggcc agcgcaaacc aagaatttgt
    28621 tgcgccagac cgttacacca gaaaataccg ccctcttccg tggtcagcac cactgcgtcg
    28681 ggcagcgact ccgcgccgct acgaaagcgt ttaatcagat tgcccagttc acggcggcgt
    28741 tttttatttc gcagctgcat ctggtgtaag ccgtatagta gcggttccca gctaccacgc
    28801 cccggtggcg gggtcatact acgatccacc cacagccacc atgaaaggcg caataaattc
    28861 cagaaatgcc agataagcag tcccgttacc gatgccagca aaaaccaggg caggtaacca
    28921 aaaaatgcac cctggatgaa agccgggagg cagcaaagta gcagctccag caccagcctt
    28981 ttccacgaca gccgttccag cacgtaagat actccagtta agaaatcata agccctgctc
    29041 tgcgtccgat gagcaaggcg ttaaaagcgg gttgaaaaac gatatcctgt accgcgcacg
    29101 gtctgcacca tgcggtcatg cccgccgggc tccagtgctt tacgcaggcg acgaatgtgg
    29161 acatcgaccg tgcggtcttc cacatacacg ttagttcccc agacgtggtt taacagctgc
    29221 tcgcggctgt acacgcgctc aggatgcgtc ataaaaaagt gcagcagttt aaattctgtc
    29281 ggccccatct ccagcggctc ttcgcccgcc atcactcggt gagatggcgg gtcgagactt
    29341 aatccctgca tctcaatcac ctcttccacc gccattggcg aaatacggcg cattaccgct
    29401 ttgattcgcg ccaccagctc cttcggcgaa aacggcttgg tgatatagtc atccgcgccg
    29461 gtttcaaggc cgcgcacgcg atcttcttct tcccctctgg cggtcaacat caccactgga
    29521 atatcccggg tcatcgactc gcgcttgagg tgtttgatga actggatacc ggagccgcca
    29581 ggtaacatcc agtcaaggag aattaaatcc ggccagggtt cattcagttg attcacagca
    29641 ctgtcataat cttccgcttc gaccggctga aagccatttt gttcgagcac gaagcagacc
    29701 atttcgcgaa ttggagcttc atcttctacg accagaatac gtctcgccat gatttgccct
    29761 gttgtaataa ataggttgcg atcattaatg cgacgtcatt atgcgtcaga tttatgacag
    29821 atttatgaaa agctcgtcgc acatatcttc aggttattga tttccgtggc gcagaaaaaa
    29881 gcaaatggca catctgtttg ggtataatcg cgcccatgct ttttcgccag ggaaccgtta
    29941 tgcgcatcct tcacacctca gactggcatc tcggccagaa cttctacagt aaaagccgcg
    30001 aagctgaaca tcaggctttt cttgactggc tgctggagac agcacaaacc catcaggtgg
    30061 atgcgattat tgttgccggt gatgttttcg ataccggctc gccgcccagt tacgcccgca
    30121 cgttatacaa ccgttttgtt gtcaatttac agcaaactgg ctgtcatctg gtggtactgg
    30181 caggaaacca tgactcggtc gccacgctga atgaatcgcg cgatatcatg gcgttcctca
    30241 atactaccgt ggtcgccagc gccggacatg cgccgcaaat cttgcctcgt cgcgacggga
    30301 cgccaggcgc agtgctgtgc cccattccgt ttttacgtcc acgtgacatt attaccagcc
    30361 aggcggggct taacggtatt gaaaaacagc agcatttact ggcagcgatt accgattatt
    30421 accaacaaca ctatgccgat gcctgcaaac tgcgcggcga tcagcctctg cccatcatcg
    30481 ccacgggaca tttaacgacc gtgggggcca gtaaaagtga cgccgtgcgt gacatttata
    30541 ttggcacgct ggacgcgttt ccggcacaaa actttccacc agccgactac atcgcgctcg
    30601 ggcatattca ccgcgcacag attattggcg gcatggaaca tgttcgctat tgcggctccc
    30661 ccattccact gagttttgat gaatgcggta agagtaaata tgtccatctg gtgacatttt
    30721 caaacggcaa attagagagc gtggaaaacc tgaacgtacc ggtaacgcaa cccatggcag
    30781 tgctgaaagg cgatctggcg tcgattaccg cacagctgga acagtggcgc gatgtatcgc
    30841 aggagccacc tgtctggctg gatatcgaaa tcactactga tgagtatctg catgatattc
    30901 agcgcaaaat ccaggcatta accgaatcat tgcctgtcga agtattgctg gtacgtcgga
    30961 gtcgtgaaca gcgcgagcgt gtgttagcca gccaacagcg tgaaaccctc agcgaactca
    31021 gcgtcgaaga ggtgttcaat cgccgtctgg cactggaaga actggatgaa tcgcagcagc
    31081 aacgtctgca gcatcttttc accacgacgt tgcataccct cgccggagaa cacgaagcat
    31141 gaaaattctc agcctgcgcc tgaaaaacct gaactcatta aaaggcgaat ggaagattga
    31201 tttcacccgc gagccgttcg ccagcaacgg gctgtttgct attaccggcc caacaggtgc
    31261 ggggaaaacc accctgctgg acgccatttg tctggcgctg tatcatgaaa ctccgcgtct
    31321 ctctaacgtt tcacaatcgc aaaatgatct catgacccgc gataccgccg aatgtctggc
    31381 ggaggtggag tttgaagtga aaggtgaagc gtaccgtgca ttctggagcc agaatcgggc
    31441 gcgtaaccaa cccgacggta atttgcaggt gccacgcgta gagctggcgc gctgcgccga
    31501 cggcaaaatt ctcgccgaca aagtgaaaga taagctggaa ctgacagcga cgttaaccgg
    31561 gctggattac gggcgcttca cccgttcgat gctgctttcg caggggcaat ttgctgcctt
    31621 cctgaatgcc aaacccaaag aacgcgcgga attgctcgag gagttaaccg gcactgaaat
    31681 ctacgggcaa atctcggcga tggtttttga gcagcacaaa tcggcccgca cagagctgga
    31741 gaagctgcaa gcgcaggcca gcggcgtcgc gttgctcacg ccggaacaag tgcaatcgct
    31801 gacagcgagt ttgcaggtac ttactgacga agaaaaacag ttacttaccg cgcagcagca
    31861 agaacaacaa tcgctaaact ggttaacgcg tcaggacgaa ttgcagcaag aagccagccg
    31921 ccgtcagcag gccttgcaac aggtgttagc cgaagaagaa aaagcgcaac ctcaactggc
    31981 ggcgcttagt ctggcacaac cggcacgaaa tcttcgtcca cactgggaac gcatcgcaga
    32041 acacagcgcg gcgctggcgc atattcgcca gcggattgaa gaagtaaata ctcgcttaca
    32101 gagcacaatg gcgcttcgcg cgagcattcg ccaccatgcg gcgaagcagt cagcagaatt
    32161 acagcagcag caacaaagcc tgaatacctg gttacaggaa cacgaccgct tccgtcagtg
    32221 gaacaacgaa ctggcgggtt ggcgtgcgca gttctcccaa caaaccagcg atcgcgagca
    32281 tctgcggcaa tggcagcaac agttaaccca tgctgagcaa aaacttaatg cgcttgcggc
    32341 gatcacgttg acgttaaccg ccgatgaagt tgctaccgcc ctggcgcaac atgctgagca
    32401 acgcccactg cgtcagcgcc tggtcgcgct gcatgggcag attgttcccc aacaaaaacg
    32461 tctggcgcag ttaatggtca ctatccagaa tgtcactctg gaacagacgc aacgtaatgt
    32521 cgcacttaac gaaatgcgcc agcgttataa agaaaagacg cagcaacttg ccgatgtgaa
    32581 aaccatttgc gagcaggaag cgcgcatcaa aacgctggaa gcccagcgtg cacagttaca
    32641 ggcgggtcag ccttgtccac tttgtggttc caccagccat ccggcggtcg aggcgtatca
    32701 ggcgctggag cctggcgtta atcagtctcg attactggcg ctggaaaacg aagttaaaaa
    32761 gctcggcgaa gaaggtgcgg cgctgcgcgg gcaactggat gcattaacga agcagcttca
    32821 gcgcgatgaa aacgaagcgc aaagcctccg acaagataag caagcactta ctcaacaatg
    32881 gcaagccgtc acggccagcc tcaatatcac cttgcagcca caggacgata ttcaaccgtg
    32941 gcttgatgca caagatgagc acgaacgcca gctgcggtta ctcagccaac gacatgaatt
    33001 acaagggcag attgccgcgc ataatcagca aattatccag tatcaacagc aaattgaaca
    33061 acgccagcag cagctcttaa cggcattggc gggttatgca ctgacattgc cacaggaaga
    33121 tgaagaagag agctggttgg cgacacgtca gcaagaagcg cagagctggc agcaacgcca
    33181 gaacgaatta accgcgctgc aaaaccgtat tcagcagctg acgccgattc tggaaacgtt
    33241 gccgcaaagt gatgatctcc cgcacagcga agaaactgtg gcgctggata actggcggca
    33301 ggttcatgaa caatgtctcg cattacacag ccagcagcag acattacagc aacaggatgt
    33361 tctggcggcg caaagtctgc aaaaagccca ggcgcagttt gacaccgcgc tacaggccag
    33421 cgtctttgac gatcagcagg cgttccttgc ggcgctaatg gatgaacaaa cactaacgca
    33481 gctggaacag ctcaagcaga atctggaaaa ccagcgccgt caggcgcaaa cgctggtcac
    33541 tcagacagca gaaacgctgg cacagcatca acaacaccga cctgacgggt tggctctcac
    33601 tgtgacggtg gagcagattc agcaagagtt agcgcaaact caccaaaagt tgcgtgaaaa
    33661 cacgacgagt caaggcgaga ttcgccagca gctgaagcag gatgcagata accgtcagca
    33721 acaacaaacc ttaatgcagc aaattgctca aatgacgcag caggttgagg actggggata
    33781 tctgaattcg ctaataggtt ccaaagaggg cgataaattc cgcaagtttg cccaggggct
    33841 gacgctggat aatttagtcc atctcgctaa tcagcaactt acccggctgc acgggcgcta
    33901 tctgttacag cgcaaagcca gcgagacgct ggaagtcgag gttgttgata cctggcaggc
    33961 agatgcggta cgcgataccc gtaccctttc cggcggcgaa agtttcctcg ttagtctggc
    34021 gctggcgctg gcgctttcgg atctggtcag ccataaaaca cgtattgact cgctgttcct
    34081 tgatgaaggt tttggcacgc tggatagcga aacgctggat accgcccttg atgcgctgga
    34141 tgccctgaac gccagtggca aaaccatcgg tgtgattagc cacgtagaag cgatgaaaga
    34201 gcgtattccg gtgcagatca aagtgaaaaa gatcaacggc ctaggctaca gcaaactgga
    34261 aagtacgttt gcagtgaaat aactattcag caggataatg aatacagagg ggcgaattat
    34321 ctcttggcct tgctggtcgt tatcctgcaa gctatcactt tattggctac ggtgattggt
    34381 agccgttctg gtagttgtga tggtggtatg aaaaaagtca ttttatcttt ggctctgggc
    34441 acgtttggtt tggggatggc cgaatttggc attatgggcg tgctcacgga gctggcgcat
    34501 aacgtaggaa tttcgattcc tgctgccggg catatgatct cgtattatgc gctgggagtg
    34561 gtggtcggtg cgccaatcat cgcactcttt tccagccgct actcactcaa acatatcttg
    34621 ttgtttctgg tggcgttgtg cgtcattggc aacgccatgt tcacgctctc ttcgtcttac
    34681 ctgatgcttg ccattggtcg cctggtttcc ggctttccgc atggcgcatt ttttggcgtc
    34741 ggcgcgatcg tgttatcaaa aattatcaaa cccggaaaag tcaccgccgc cgtggcgggg
    34801 atggtttccg ggatgacagt cgccaatttg ctgggcattc cgctgggaac gtatttaagt
    34861 caggaattta gctggcgtta caccttttta ttgatcgctg tttttaatat tgcggtgatg
    34921 gcatcggtct atttttgggt gccggatatt cgcgacgagg cgaaaggaaa gctgcgcgaa
    34981 caatttcact ttttacgcag cccggccccg tggttaattt tcgccgccac gatgtttggc
    35041 aacgcaggtg tgtttgcctg gttcagctac gtaaagccat acatgatgtt tatttccggt
    35101 ttttcggaaa cggcgatgac ctttattatg atgttagttg ggctagggat ggtgttggga
    35161 aatatgctaa gtggcaggat ttcaggacgt tattcaccac tgcgcattgc agcagtgact
    35221 gactttataa ttgtactggc actgctgatg ctctttttct gcggcggcat gaaaacaacg
    35281 tcgcttattt ttgcttttat ttgttgcgcg gaattatttg ccctttcagc acctctgcaa
    35341 atattgttac tacaaaacgc caaaggcgga gagttattag gtgccgcagg tgggcaaata
    35401 gcgtttaacc tcggtagcgc cgtcggcgca tattgcggag gtatgatgct gacgctgggg
    35461 ctggcatata attacgtggc gctgcctgcc gccctgcttt cgtttgctgc gatgtcatcg
    35521 ttgctgctgt atggtcgcta taagcgccag caagcggcgg atagtccggt gctggcgaaa
    35581 ccactggggt agattactgt cacggtgcgc gttgcttatt gccggatgcg gcgtgaacgc
    35641 cttatccgcc ctacgcggtt ctggcacatt ttgtagatac tgataagacg cggcaagcgt
    35701 cgcatcaggc atgatgcgcc aattgcctac gttttttact cttgtggcca taaccacgca
    35761 gcgccgcgta cgccgctgga atcaccgtgc ttcgccttac gcaccggcgt ttcacattcg
    35821 ccgccgaaga caaattgttt aatcaactgc ggaaccgttt gatataaacg gtctacattg
    35881 ctcatcccgc cccccaggac aatcacatcc ggatcgagaa tattcacgac atgtgccagc
    35941 gattttgcca gccgcagctc gtagcgacgc aatgccagtt ccgctaccgg atcgctttct
    36001 tcaaccaggc tgataatttc actgcctttc agcgcatgtc cgctcaaacg acgataatcc
    36061 gtcgcgaatc cagtgcccga aataaaggtt tcaatacaac cttgtttacc gcaataacaa
    36121 gggacttcct cgcgataacg cagttcgtct tcgtccatcc acggtagcgg attgtgtccc
    36181 cactcacctg ccgtgccatt gccgccgata tgcgcccgcc cattgaatgc cacgcccgcg
    36241 ccgcatcccg tgccgataat cacggcaaat accgtctgcg ctcccgctgc cgcgccatct
    36301 accgcttctg aaaccgccag acagttggcg tcatttgcca gccgcacttc ccgctgcaac
    36361 cgcgcgctta agtctttatc gaatggctga ccgttgagcc aggttgaatt ggcattcttc
    36421 accacaccgg tgtagggcga aatcgaacct ggaatgccca tacctaccgt tccgcgctgc
    36481 cctgtcgcct gctccgccat atcaaccaac gtggcgatcg tttcaatagt ttgccggtaa
    36541 tcatcacgcg gcgtgggcag acgatggcgg tacaactgct cccctgcatc gcccagtgca
    36601 atcacttcag ttttggtgcc gcctaaatcg atacctatac gcacggtact ctccttattt
    36661 ttttcaatat caatagcgta gagacggaca accggattgg caatgcaagg ccgccgacaa
    36721 ttcgttatca tgcccgctaa atttaacgac aaggccgtgg aaattatcat gctgtggttc
    36781 aaaaatttaa tggtttaccg tcttagccgc gagatttcgc tgcgtgcaga agagatggaa
    36841 aaacagctag cctcgatggc atttacccca tgcggcagcc aggacatggc gaagatgggc
    36901 tgggttcctc cgatgggatc gcacagcgat gcgttaacgc acgttgccaa tggtcaaatt
    36961 gttatctgcg cgcgcaaaga agaaaaaatc ctcccgtctc cggtgattaa acaggcgctg
    37021 gaagcgaaaa tcgccaaact ggaagcggaa caggcgcgta agctgaagaa aaccgaaaaa
    37081 gattcgctga aagacgaagt gctgcactct ctgctgccgc gtgctttcag ccgttttagc
    37141 cagacaatga tgtggatcga cacggttaac ggtttgatta tggtggactg cgccagtgcc
    37201 aaaaaagcgg aagatacgct ggcattactg cgtaaaagcc tggggtcgtt accggttgta
    37261 ccattgagca tggaaagccc gattgaactg acgctgaccg aatgggttcg ctccggtagt
    37321 gcggcacagg gcttccagct gcttgatgaa gccgagctga aatcgttgct ggaagatggc
    37381 ggcgtgatcc gcgcgaagaa acaagatctg accagcgaag agatcaccaa tcacattgaa
    37441 gccggaaaag tggtgactaa actggcgctc gactggcagc agcgcattca gtttgtgatg
    37501 tgcgacgatg gttcgctcaa gcgtctgaag ttctgcgacg agctgcgcga tcaaaacgaa
    37561 gatatcgacc gtgaagattt cgcccagcgt tttgatgccg atttcatcct gatgactggt
    37621 gaactggcag cgttaattca aaacctgatt gaaggattag gtggcgaagc acaacgttaa
    37681 ttgctgattt tcctttaatg ccggatgcga cgcctgccgc gtcttatccg gcgtacgaag
    37741 ccacaccagg catataatta ttcgctacgg cgggcaataa tttttagcgc agcaatatta
    37801 tgcgttttat gctgtaactt gctccatgga cgttgtgtca ttgtttttcc tcaagccagt
    37861 gacgcgcttc actgcgaatt ttccgcattg ccccggtttt cactaaatcc cgtaacagcg
    37921 aaagatcggg ttgttcgaca aataagagta atgatggatc ctcttttaac cacgtcgttt
    37981 tcacatcggc agcgagctgt ggattattca tagctagcga ccgatgccga gactcggtca
    38041 atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa tgccgtttgg cgcaaatgat
    38101 cattgcgact ttgcagcaat gcacgccacc attcatcatc tgatttatct tttatcgtag
    38161 ccagttccgc aacaatatct ggagatcttc actgatattt atatcaacct tgatgaatta
    38221 tgaatatacc ggaaattgaa aaaatcgcag caaaggaaat attattatgg gttaaagaga
    38281 ggatatttca gaaaaatgcc ctcccgttca aggggaaggc gaggaattac agatagcggc
    38341 acagataaga ggtgggttcg gcaacttgca gatgaaactc actgtgaccg ggaacattaa
    38401 acaccgaacc ggcttcatac acctgccagt cggtcgcgtc aggcagtaac acattcagcg
    38461 cgccactgat taccgtcatc tcttccggct cagcggtgct gaaggtgtat tcgccttcaa
    38521 ccataacacc cacgctggcg cgaccagtgc tgctgctgga aaagccgatt gatttcactt
    38581 tgccggaaaa gtactcatta ctttgaagca taaactggcc ctgtctggta gttgaacatc
    38641 tacttcacta taggggccag aggcgctggc tgtcacgcaa aattacacca gtaattccgc
    38701 agccagccgt gcaatcaata cgttagacag caagacagga acatcgagct gtttttgcag
    38761 taaatcgcga tgacgttggt gaaatcctaa acaatccagc atgatgacat cagccccttt
    38821 tgccagtaat tctttcccgg catcaatgat tttttgttct gaatcatgaa tggggttgcc
    38881 caatgaaaat accggcgatt tctgcaaaat ttgccatttt tgcgcctgaa cggtcagcat
    38941 ctcctcaacc ggaacgataa cccccacctg atgatcttca acaatagagg aaaccagtgg
    39001 aggcaatatt cgcgacggct caagaaagat cgtattacgc gcagtcatac tactaatgtt
    39061 tgctgtactc attaataaaa tgacgtcata accctgatta tcgagcactt caaccacacc
    39121 ttgcaggtct cgctccactt tacgacgcga aacatgggcc agctggttgt catttaataa
    39181 tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc gccatcactt cttcacgact
    39241 taacttcccc agcaagctat gatgggaaat attatcttcg tcaatgtatt ccgtcaggag
    39301 cggcaaaacc tcctgcatag gtacaatgcc gatggtcagg atcgccaacg acgcactcat
    39361 tgttcatcca ccttactttt ctttcgtcgt taattaccgg gcaagtgtga agcaccactg
    39421 ctgacactta cttgtgctac aaatgacaaa aagcgtagca gcagacgtgc acggcataat
    39481 gtaaagattc caaatgattc cagtaatgga ttgttattgt ttaatattct aattattaga
    39541 aaaacatgaa ttatgaaaaa tgtgacgcag atcccttgta gtggcactca gaatcccttc
    39601 cgagcagtct gatgcggtca ctctttgtca gggtcctcat agcgtttttt cgcatccatc
    39661 gcttcctgag ccgtcggatg ttcactgatc aacgagtctg gtttaggatg atcggctctg
    39721 agttggtacc aggttaccgt ctgtcctggc tttccttttt caatcgtcac tatatatgct
    39781 tcacgaggat aaggcggttt cgttggcatc gttcttcctt atttcacggg atgaacgtta
    39841 agtataggcg ctctaaaatc aacaattgat cgtctgtgcc agggcgctac gaatttcaga
    39901 aatcacctgg ctgggttcgt ttgttgcgtc gatgataata tgcgcaactt cgcgatatag
    39961 cgcatcgcgt tcttccagca cttcctgaac ttcttcgctc agcggttttc ccgttaaggt
    40021 tggccgtaaa tcttcttccg gtgcagcttg cagtcggtta accaggactg atactggcgc
    40081 acacaaataa accacgatcc cgttattttg catgaagtga cgattaaatt ccgtcagaat
    40141 aatgccgccg cctgtagcga taacggtgga tggcgcagtt accgcttcca gcgccgccgt
    40201 ttctctggcg cgaaatcccg cccactcttc cctttcgacg atctccgcga ccgtcatatt
    40261 gagctgtgat tgcaaccact gatcggtatc gacaaaccga cggttaagcg aatcggcaag
    40321 ggccattccg accgttgttt taccacagcc ccgaggcccg atcagaaaaa gaggttgtgt
    40381 catcgtgggt tttccccaat aggtcgcaat gcggcgaaag ccggtgtcat gagaatagcg
    40441 atcataccat caaactagta caatttcgat tgtaaagaaa aaattccact taaagtaaaa
    40501 atctcaatac atcccttact ataccaataa atattcaaga atgaagtgta aataataaat
    40561 tacatttagc cacgactacg ttgcacttcc agccaccact tctccagctc cgcggcaaag
    40621 gcctggcggt cacgttgtga aaggctatct ggtccgccgg tctggatccc actggcacgt
    40681 aaggtatcca taaaatcgcg catcgtcagg cgctcacgaa tggtcgctgg cgtgtaacgt
    40741 tcgccgcgcg gattaagcgc cgctgcgcct ttctcgatgg cttcagcagc caaaggtata
    40801 tctgcggtga tcaccaaatc gcccgcttca cactgccgga caatttcgtt atcggcaacg
    40861 tcgaaacctg ccgcgacgcg cagcgtacga ataaatcgcg atggcggcac gcgtaaactc
    40921 tggtttgcta ccagtaccag cggcatctgc atacgttccg ccgcgcgata caaaatctct
    40981 ttaattacat tgggacacgc gtcggcatcc acccaaattg tcataaagtc atcctttgtt
    41041 gggtaatcct ctattgtgtc gcgcttttgc cttccggcat agttctgttt atgcttctgc
    41101 cagcgattat caaaacaatg aatttcacgg caggagtgag gcaatggaaa agaaaatcgg
    41161 ttttattggc tgcggcaata tgggaaaagc cattctcggc ggtctgattg ccagcggtca
    41221 ggtgcttcca gggcaaatct gggtatacac cccctccccg gataaagtcg ccgccctgca
    41281 tgaccagttc ggcatcaacg ccgcagaatc ggcgcaagaa gtggcgcaaa tcgccgacat
    41341 catttttgct gccgttaaac ctggcatcat gattaaagtg cttagcgaaa tcacctccag
    41401 cctgaataaa gactctctgg tcgtttctat tgctgcaggt gtcacgctcg accagcttgc
    41461 ccgcgcgctg ggccatgacc ggaaaattat ccgcgccatg ccgaacactc ccgcgctggt
    41521 taatgccggg atgacctccg taacgccaaa cgcgctggta accccagaag ataccgctga
    41581 tgtgctgaat attttccgct gctttggcga agcggaagta attgctgagc cgatgatcca
    41641 cccggtggtc ggtgtgagcg gttcttcgcc agcctacgta tttatgttta tcgaagcgat
    41701 ggccgacgcc gccgtgctgg gcgggatgcc acgcgcccag gcgtataaat ttgccgctca
    41761 ggcggtaatg ggttccgcaa aaatggtgct ggaaacggga gaacatccgg gggcactgaa
    41821 agatatggtc tgctcaccgg gaggcaccac cattgaagcg gtacgcgtac tggaagagaa
    41881 aggcttccgt gccgcagtga tcgaagcgat gacgaagtgt atggaaaaat cagaaaaact
    41941 cagcaaatcc tgatgacttt cgccggacgt caggccgcca cttcggtgcg gttacgtccg
    42001 gctttctttg ctttgtaaag cgccaaatct gccgatttca accactcacg atagtgactc
    42061 atttgtgggt tcagcggcgc aacccccaca ctaatccgta aagttacctg tggcgtattc
    42121 ggcaagcgta acgtgtttag cccttcacgc acccgtaaca tggcggtaat ggcgctctca
    42181 gctggcgtac cggacatgat tactgcaaac tcatcgccgc caaaccgacc aatcacatcg
    42241 ctaccgcgca gggtaatttg taactgtcgg gtaagcgcca caatcgcttc atcgcccaca
    42301 tcatgacccc aggtatcgtt gatgctcttg aaatggtcga tatcgataat cagtaacgtt
    42361 gcatcgcgat tatgccgccg acagttatca aattcattgc gtaacatagt ttcccaatga
    42421 cgtcggttat acacgcccgt catgccgtcg cgggtactca tgacctgcaa cctgcgttta
    42481 tgttccgcca gtttggttgc cgtctggtag ctgacccagc caaacagcag aggataaacg
    42541 acaataatgg gaagggagag ccaccattcc agcggcgcac tattgaacga cacggtaatg
    42601 cccgtaagct cgagggtgac aaggcaggaa accaccatca acaccagacc cgcgacaaac
    42661 agacgggggc cgcctgcccc catcaaattc agacacataa tcatcaacat agcggtggaa
    42721 ggcagcacgt ttacgcccat tacgcctacc cacattcccg ctaatactgc atcggttttt
    42781 aagttgtaaa tttcccggct aagcggatcg acggccctgc tcgctatctg ccaggctaaa
    42841 tgcggccaga cgaacgccca gccgaccaac accagccacc accagcccgg cggcgggtgc
    42901 gaaaccagcg ttgaagcaat cggtaagaac atgccagcca ggccaaccgc acggggtagt
    42961 ctgacgcgac gggcgaagcg cagcccggac cgctgatgtt cgttttgagg agttaaagga
    43021 ggttcctccc catgcgccgc cgcttttttg aaaaagtttt catcattcat tatttttggg
    43081 aacattcaga aacaattttc ccaaattata gagacggggc taaaggcaga agtatgaaat
    43141 ttcgggtgag ccactttcgt agctcacccg ttagcactat caggtatcag gcggctttct
    43201 tgaggcaggc actcataaac ttattacgat catcaccttt cagagattgt tgcgttgctt
    43261 gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa acttttttca ccaggcgcag
    43321 acttgctgtt cttcaggcaa taactcatgt aggtcttacg agcatcccct ttcaacgcct
    43381 gcgccgtcgc ctgctgatta caggatgtca tacgctgttg ttgtggggtt aaggttctct
    43441 cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag caaggtaacc agtaatgtta
    43501 ttttcatagc accatccctc ttcatgtttt aaccatgagc gtatgcgccc gtgatctgcc
    43561 attaagtctg gttgctaaca gcaaaaaaac cacccggcag cgaaaattca ctgccgggcg
    43621 cggttttatt tcagccccag ggcggctttc atggtgtaga agagatcggt ctggtcggtc
    43681 agtccaacaa cattggcggc atgcgggcca tacgccgcaa tacgcaactg actgccggta
    43741 tgttcttgtg aatcctcttc ggagttcccg tagctcatca ccatcactgc gccatctttg
    43801 gtatttagcg cctgggtgag gcccggagct ttggtatccg gcgcgacaat ctggctggcg
    43861 tgggcgtgat cagcggtgac tatgaccagc gtgttgccat cctttttagc gaattccagc
    43921 gcccgttgta cggcttcatc gagatcgacc gtctcgccaa tttgcccaca aggattcgca
    43981 gcgtgatcct gtttatcgat tgacgcacct tcaacttgca ggaaaaatcc tttctcattt
    44041 ttactcaaca attcaatggc tttgtcggtc atctgcgcca gggttggtac actgtcatta
    44101 cgttgcggat taggcgtaca ggtgactgcg ggcttatcga tattgccgtg gtacgttgct
    44161 ttcggtccta gccagcgcac tggcatattg ccgtcagcaa acagtccaag cagaggtttt
    44221 tgctgattcg cttccgtcac cgaattcagt gaggcagcat cgctgaccaa ctgataacca
    44281 cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt caccggcggt tgccgtttca
    44341 gcaaaggttt ttgcgccacc gccaagcgta acgtcggcac gagcgttaag cagctgttcg
    44401 gtaatcgatc cttttccgcc tttttccaga gcgttacccg gacatttttc actggtcgcg
    44461 ctcggaccgt agcatttgcg cgaggtcaca tgcgccacca gcgcagcggg cgtggcatcc
    44521 tgcaactctg cggtagaaac gttaccggtc gccagacctg cggcttttgc catttccaga
    44581 atcgttgggt gatctttttc gtgaatatcg acgcccagcg cgccgttata ggttttgaca
    44641 ccggttgacc aggcggttgc tgatgcagcc gaatcggtga cgtagtccgg tttgccggtt
    44701 tttttattca gcgcatagtg agtgtattgc ccggtaagcg gtaaggcatc gataccttta
    44761 aaaaagccgc ccgcaccttc ggcataatta cgtgcggctg taatttccga atcccccatc
    44821 ccatcgccaa tcagcaaaat aatatttttt gcaggtttat cgctaagaga atcacgcaga
    44881 gcggcagtct gatcacccgt taaacggcga gcaccgccgg atgcagtaat atcgccctga
    44941 gcagcccggt tttccagaac aggcatttct ggtgtccggg cttttgtcac aggggtaaac
    45001 agtaacgata agagtgccag tgcaatagtg ctttgtttca ctttattttc tccatgtaca
    45061 aatacattaa aaaataaaaa caaagcgact ataagtctcg gccgtgacaa ctttatgaca
    45121 gctgttgaaa agattaactt tttactgacg aggatgcttc aataacttct ttacgtaatc
    45181 gcgcagcagc tccgtatcgt cgtcaggaat gctggcatcg ggctttacct cgtacagcgc
    45241 cccctctacc tgatcaatca agcgctgttg gtcattttgc gccatattgc gaagcattgc
    45301 agtgacgata atctccaaag cttctacctg cgcacacagt tctttcgact cttcttcttt
    45361 ttgggcaagc ttaaataaca actcagcaat gagatttttc atgtctgtat ttccttatcc
    45421 aaagtatgga gaagttaaca ttatgtttca caacttcata gtgtctggta gtattatttt
    45481 tatgacaaag gaaatacttt gcgcaaatat tttttgtttg attaccagcg aaacgtttct
    45541 cttatttaaa taattaccca cacaaaatat aggcttttaa ttatcaacct ttattataaa
    45601 t
//
LOCUS       DAZGTR010000022        45437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGTR010000022 DAZGTR010000000
VERSION     DAZGTR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.022"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..915
                     /locus_tag="ACHLCY_001426"
     CDS             <1..915
                     /locus_tag="ACHLCY_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0368721.1"
                     /translation="FSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESG
                     DIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVD
                     YAKAYALFNQAAQHGVNLARSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFL
                     ATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVT
                     FSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATV
                     RTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(971..1513)
                     /locus_tag="ACHLCY_001427"
     CDS             complement(971..1513)
                     /locus_tag="ACHLCY_001427"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0368722.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1497..1808)
                     /locus_tag="ACHLCY_001428"
     CDS             complement(1497..1808)
                     /locus_tag="ACHLCY_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0368723.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1993..2883)
                     /gene="cyoE"
                     /locus_tag="ACHLCY_001429"
     CDS             complement(1993..2883)
                     /gene="cyoE"
                     /locus_tag="ACHLCY_001429"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP0368724.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2895..3224)
                     /gene="cyoD"
                     /locus_tag="ACHLCY_001430"
     CDS             complement(2895..3224)
                     /gene="cyoD"
                     /locus_tag="ACHLCY_001430"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP0368725.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3224..3838)
                     /gene="cyoC"
                     /locus_tag="ACHLCY_001431"
     CDS             complement(3224..3838)
                     /gene="cyoC"
                     /locus_tag="ACHLCY_001431"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP0368726.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3828..5819)
                     /gene="cyoB"
                     /locus_tag="ACHLCY_001432"
     CDS             complement(3828..5819)
                     /gene="cyoB"
                     /locus_tag="ACHLCY_001432"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP0368727.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5841..6788)
                     /gene="cyoA"
                     /locus_tag="ACHLCY_001433"
     CDS             complement(5841..6788)
                     /gene="cyoA"
                     /locus_tag="ACHLCY_001433"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP0368728.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7248..8723)
                     /gene="ampG"
                     /locus_tag="ACHLCY_001434"
     CDS             complement(7248..8723)
                     /gene="ampG"
                     /locus_tag="ACHLCY_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP0368729.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTGVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8767..9345)
                     /gene="yajG"
                     /locus_tag="ACHLCY_001435"
     CDS             complement(8767..9345)
                     /gene="yajG"
                     /locus_tag="ACHLCY_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0368730.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9650..9967
                     /gene="bolA"
                     /locus_tag="ACHLCY_001436"
     CDS             9650..9967
                     /gene="bolA"
                     /locus_tag="ACHLCY_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP0368731.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10311..11609
                     /gene="tig"
                     /locus_tag="ACHLCY_001437"
     CDS             10311..11609
                     /gene="tig"
                     /locus_tag="ACHLCY_001437"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0368732.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            11855..12478
                     /gene="clpP"
                     /locus_tag="ACHLCY_001438"
     CDS             11855..12478
                     /gene="clpP"
                     /locus_tag="ACHLCY_001438"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP0368733.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12604..13878
                     /gene="clpX"
                     /locus_tag="ACHLCY_001439"
     CDS             12604..13878
                     /gene="clpX"
                     /locus_tag="ACHLCY_001439"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP0368734.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14066..16420
                     /gene="lon"
                     /locus_tag="ACHLCY_001440"
     CDS             14066..16420
                     /gene="lon"
                     /locus_tag="ACHLCY_001440"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP0368735.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16611..16901
                     /gene="hupB"
                     /locus_tag="ACHLCY_001441"
     CDS             16611..16901
                     /gene="hupB"
                     /locus_tag="ACHLCY_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP0368736.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17093..18964
                     /gene="ppiD"
                     /locus_tag="ACHLCY_001442"
     CDS             17093..18964
                     /gene="ppiD"
                     /locus_tag="ACHLCY_001442"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0368737.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSLYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKVVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFTLPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19115..19486
                     /gene="ybaV"
                     /locus_tag="ACHLCY_001443"
     CDS             19115..19486
                     /gene="ybaV"
                     /locus_tag="ACHLCY_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP0368738.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19580..19978
                     /gene="fadM"
                     /locus_tag="ACHLCY_001444"
     CDS             19580..19978
                     /gene="fadM"
                     /locus_tag="ACHLCY_001444"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP0368739.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20030..20725)
                     /gene="queC"
                     /locus_tag="ACHLCY_001445"
     CDS             complement(20030..20725)
                     /gene="queC"
                     /locus_tag="ACHLCY_001445"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP0368740.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20790..22490)
                     /gene="ybaE"
                     /locus_tag="ACHLCY_001446"
     CDS             complement(20790..22490)
                     /gene="ybaE"
                     /locus_tag="ACHLCY_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP0368741.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22590..23408
                     /gene="cof"
                     /locus_tag="ACHLCY_001447"
     CDS             22590..23408
                     /gene="cof"
                     /locus_tag="ACHLCY_001447"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP0368742.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23561..24019
                     /gene="decR"
                     /locus_tag="ACHLCY_001448"
     CDS             23561..24019
                     /gene="decR"
                     /locus_tag="ACHLCY_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP0368743.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24049..25821
                     /gene="mdlA"
                     /locus_tag="ACHLCY_001449"
     CDS             24049..25821
                     /gene="mdlA"
                     /locus_tag="ACHLCY_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0368744.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHRALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVASNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            25814..27595
                     /gene="mdlB"
                     /locus_tag="ACHLCY_001450"
     CDS             25814..27595
                     /gene="mdlB"
                     /locus_tag="ACHLCY_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0368745.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27776..28114
                     /gene="glnK"
                     /locus_tag="ACHLCY_001451"
     CDS             27776..28114
                     /gene="glnK"
                     /locus_tag="ACHLCY_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP0368746.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28144..29430
                     /gene="amtB"
                     /locus_tag="ACHLCY_001452"
     CDS             28144..29430
                     /gene="amtB"
                     /locus_tag="ACHLCY_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP0368747.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWGVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29478..30338)
                     /gene="tesB"
                     /locus_tag="ACHLCY_001453"
     CDS             complement(29478..30338)
                     /gene="tesB"
                     /locus_tag="ACHLCY_001453"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP0368748.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30556..31128
                     /gene="ybaY"
                     /locus_tag="ACHLCY_001454"
     CDS             30556..31128
                     /gene="ybaY"
                     /locus_tag="ACHLCY_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP0368749.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31159..31470)
                     /locus_tag="ACHLCY_001455"
     CDS             complement(31159..31470)
                     /locus_tag="ACHLCY_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP0368750.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31633..31729
                     /gene="ffs"
                     /locus_tag="ACHLCY_001456"
     ncRNA           31633..31729
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHLCY_001456"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            31849..32202
                     /gene="ybaA"
                     /locus_tag="ACHLCY_001457"
     CDS             31849..32202
                     /gene="ybaA"
                     /locus_tag="ACHLCY_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP0368751.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32244..33794)
                     /gene="pdeB"
                     /locus_tag="ACHLCY_001458"
     CDS             complement(32244..33794)
                     /gene="pdeB"
                     /locus_tag="ACHLCY_001458"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP0368752.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRLVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(33958..34428)
                     /gene="ylaC"
                     /locus_tag="ACHLCY_001459"
     CDS             complement(33958..34428)
                     /gene="ylaC"
                     /locus_tag="ACHLCY_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP0368753.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFAALAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34544..35095)
                     /gene="maa"
                     /locus_tag="ACHLCY_001460"
     CDS             complement(34544..35095)
                     /gene="maa"
                     /locus_tag="ACHLCY_001460"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP0368754.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(35267..35485)
                     /gene="hha"
                     /locus_tag="ACHLCY_001461"
     CDS             complement(35267..35485)
                     /gene="hha"
                     /locus_tag="ACHLCY_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP0368755.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(35511..35885)
                     /gene="tomB"
                     /locus_tag="ACHLCY_001462"
     CDS             complement(35511..35885)
                     /gene="tomB"
                     /locus_tag="ACHLCY_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP0368756.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(36431..39580)
                     /gene="acrB"
                     /locus_tag="ACHLCY_001463"
     CDS             complement(36431..39580)
                     /gene="acrB"
                     /locus_tag="ACHLCY_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP0368757.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDREKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(39603..40796)
                     /gene="acrA"
                     /locus_tag="ACHLCY_001464"
     CDS             complement(39603..40796)
                     /gene="acrA"
                     /locus_tag="ACHLCY_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP0368758.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            40938..41585
                     /gene="acrR"
                     /locus_tag="ACHLCY_001465"
     CDS             40938..41585
                     /gene="acrR"
                     /locus_tag="ACHLCY_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP0368759.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            41713..45075
                     /gene="mscK"
                     /locus_tag="ACHLCY_001466"
     CDS             41713..45075
                     /gene="mscK"
                     /locus_tag="ACHLCY_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP0368760.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMCNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(45314..>45437)
                     /locus_tag="ACHLCY_001467"
     CDS             complement(45314..>45437)
                     /locus_tag="ACHLCY_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0368761.1"
                     /translation="NGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT        11475 a        11177 c        11967 g        10818 t
ORIGIN      
        1 ttttctgtaa tgctgatagt ttcaccaagc gtggtggcca gcgagaaagc gcatgaattg
       61 tatgacagca tatacggtgg taaacctgct cccgatgtta taaatacact gcataaaatg
      121 gctgaatcgg gagatatcga cgcgcagagc ctgttaggct gggaatatta tcagcctcgt
      181 tatgatacca aacctgatgt tcaggaagcg attaaatggt tcgagttagc ggctaaacaa
      241 ggtgacagag aagctccgtt agcgctgggg ggtatctact acgacggtga gcaggtgcgg
      301 gtggattatg ccaaagcgta tgcactattt aatcaggccg cgcagcacgg tgtgaattta
      361 gcaaggtcca ggttgggcat tatgtacgcc aatggtcagt atgttgaggt agattgtaag
      421 aaagcgaagg aatatcttga taaaggtgtc cacatttatg gtggcccaga agactttctg
      481 gctacttgtc gaaaagacat gattgacaga aaaacggttg acgatacgtt acccgtgatt
      541 acggttaccc gttctgggat gcgagataat tttttagata aaggtttttc ctgcatggat
      601 agcctcttcg ccaccaccaa taaattaggc gaagtggcca atcttcgcgt gacattcagc
      661 attcgtcgcc catccggtaa ggagattaac caaacggttg gcttcgcgcc tttcggttta
      721 aaccggctga atattagctt tactgattac ctatttggtt catttaccag taattcgtct
      781 cttattcttt ataaaccgga atttgagcga aaatcttgtg caactgtaag gaccacaatc
      841 gttgccgcaa cggcaaccat taacggcaag gatgtggagt tgctgaaagc gggggcaatt
      901 gaacaaaagt ggtaacgctg ctcagccccc ggctgaggca aaacttgccc tggcgggtgg
      961 cgataataat ctaagccgcg agaatatgct gaatcgccaa aaacataggt acagcaccaa
     1021 aagcatttgg tgtcgctgaa agctgaacaa agccgagacg agcataaaaa ttaatggcgg
     1081 cagggtcagc atcaagataa acccctttaa ttggtaatgc ctggtgaatt atttttacat
     1141 gttcaaaaaa atcacatagt aggtcctgac caaagccccg cttttgatac ttccgtgcta
     1201 cccccaacat gactaatctg acaacaccaa tttctgaagg ttgtgaaccc tgaaggacgc
     1261 cagaaacgcg ttgtttttcc agcgaatatg ccgtaaaagt acagatgccg atcagttcac
     1321 cactttgtct gtcaataagt gctttagccg cacagtcgct attacgcaca cttttcttta
     1381 gcgatgcgcg tacaaattta tcgataacag ggttaccgca atcaaactgt ttttgcccag
     1441 gataagtaat atcagactga taattacagg agaggactat gggcagagta atctcttcat
     1501 gcttgtcgac catcgctgtt tttccttttc attaacgcct tgagggcgag cgtcggttga
     1561 gcaggttcag cgatgagttg gttcatcagt tcccagcttt gattcgaaag ctcacggcga
     1621 cgttggttat caagcacact ttcggcgcgt tccatagcag cgctcaaaat aaaagcgctt
     1681 aaatctaccc caacggcttg cgcagcttca cgaagtttgt ttttggcgtc gggactagtt
     1741 ttgagttcga cccgcgcatt tttcagcgtt ttatcgacat cgggtagagt ggctgttttt
     1801 ccaggcatta tgacctcttt gattaactga atgaaatcgt agggagtcga tctcatacat
     1861 aatacggcta tcaaccgtat gattaattta tgttcatctg ctcactctgt caagacatac
     1921 ggttattagc cgtatctctg atctcagaca ataaaaaagg tgccgtagca cctttttaat
     1981 agagaggttt tgttaccaca cagcagccag cagcgtatgc gagtccggta ccataaaatc
     2041 aacggacatc atcaccgaga gggcagtgat ggcgatgata gagaagccga acagcttgcg
     2101 cgcccagatt ctgtcatcag caactttata accgcgcaga gccataccta accaccagac
     2161 gctaaccgcc gcggcgacca ccagatattt atatccagcg taaccgccaa gagagagcat
     2221 cagcgtggca acggcaaagg cgatgatata cagcgtgatg tgattcttcg ccaccgaaat
     2281 gccttttacc actggcaata ccggaatgtt tgccgcctgg taatccttaa agcggaaaat
     2341 ggcgatggca taggagtgag gcatctgcca caggctgaag atagccagca ggatcgctgc
     2401 gccgctatcg aactcgccgg ttactgcaca gtagccaatc accggcggcg cagcgccgga
     2461 gagcgaacca atcaatgtgc cgtagactga gtggcgtttc atgtacaggc tatagacgcc
     2521 gacataaacc acaaagccca tcacccccag ccagcaggcc agcggattcg cgccaaacca
     2581 cagcagcata aagccagcaa tacccagcaa ggtggcgtac accagcgaga cagcaggaga
     2641 gatcaggcct ttaaccagca cccgattctt cgtcctttcc atctttctgt cgatatccct
     2701 gtcgatgtag ttgttaaaca cacaacccga cgccacaacc agtgacaccc caaccagcgt
     2761 gtagataaac aggggataat caatgctgcc ctttgaggcc agcaggaatc ccccaatcac
     2821 cgagatcagg ttgccaaaga tgatgcctgg tttcgttact tgcaggtatt gcttaaacat
     2881 cataaccgcc gctcttagtg catcatcatg ttgtagttga ggttccacat aatccagatg
     2941 gagcctacaa ccaggatagc gatgattagc acggtgaaga caaacgccgt catgttccag
     3001 ccttcatctg atttggtatt catgtgcagg aagcacacca gatgcaccag aacctgtacc
     3061 actgccattg ccaggattgt tcccagaatt acggccggag aggcagcccc tgtcatcacc
     3121 atccagaacg gaatcaccgt cagaatgatc gacaggataa agcctgtcat gtaggttttt
     3181 acgctgccat gggacgcgcc gctgtgatcg gtagaatgac tcattacatc gcccccatca
     3241 gataaacaac agtgaacaca cagatccaaa ccacatccag gaagtgccag aacaggctca
     3301 ggcacatgat gcgggtacgg ttagtgctgg tcaggccgcg acgagcgatt tgtaccatca
     3361 gcaccgccat ccagataaga ccggaagtga cgtgcagacc gtgcgtgccg accagcgcaa
     3421 agaacgctga caggaagccg ctgcgatccg gacccatgcc gttaacaatc aggtgatgga
     3481 attcgtagat ttccatcccg ataaatccgg caccaaacaa ccaggtcaac gccagccagg
     3541 agataacctg gcttttgttg tttttgtaca tggcgatagc cgccatgccg taggtgatgg
     3601 agctgaacaa cagcaagaaa gtttcaacca gaacgaacgg cagttcgaaa atgtccttac
     3661 ctgtcgggcc gcctgcggtg ccgttcacca gaacggcata ggtagcaaac aagattgaga
     3721 acagaatgca gtcgctcatc aggtagatcc aaaatccgaa gattttggtt ccgcctgcat
     3781 cgtggtgccc gtgttcgtgc gcgtgggcag tcgcgtgcgt caaagtatca gttgccattt
     3841 ttcagccctg ccttagtaat ctcatcgaaa tgctggtttt ccagtttttc gatttctgcc
     3901 accggcacgt agtaatccac gtcctcgtcg aagcttttca cgatccaggt gatgatcatg
     3961 cctgcgaagc caacaatcgc cagccaccag atatgccaga tcatggcgaa accgaagatg
     4021 gtggagaaag ctgcaatgac gatacctgca ccgctgtttt tcggcatatg aatttcttca
     4081 tagtggtcag gctttttata cgcttcgcct ttctctttca tttcccagaa tgcatcacgc
     4141 tcgtgaacgt gcggcacaac ggcaaagtta tagaacggag gcggggaaga ggttgcccac
     4201 tccagcgtac ggccacccca cgggtcgcca gtcaggtcac ggttctggtc gcggtcgcga
     4261 atagaaacgt acatctgaat aacgaggcag agaataccca gcgcaatcag tactgcaccg
     4321 ctagctgcaa tcatcagcat ggtgtggaac tgcgggtcaa tctgctggct caaacgacgg
     4381 gtcatgccca tgaagcccag cgcatacagc ggcataaagg caacgaagaa gccgatgatc
     4441 cagaaccaga acgcgcgttt accccaggtt tcattcagtt tgaaaccgaa ggctttaggc
     4501 caccagtagg tcatccctgc gaagcagccg aagaccacgc cgccgatgat cacgttatgg
     4561 aagtgcgcaa tcaggaacag gctgttatgc agaacgaagt ccgcgcccgg tactgccagc
     4621 agcacgccag tcatcccgcc caccgagaaa gtgacgataa aaccgatggt ccacagcatc
     4681 gcagaatgga acacgatgcg gccctgatac atggtgaaca gccagttgaa gatcttcacc
     4741 ccggtcggga tggcgataat cattgtggtg ataccaaaga aggcgtttac gttcgcgccc
     4801 gcacccatcg taaagaagtg gtgcagccaa acgatgaacg acagcacggt gatacagacg
     4861 gttgcccata ccagcgaggt ataaccaaac agacgtttac gcgagaaggt tgccgcaatt
     4921 tcggagaaca caccgaaaac aggcaggatc aggatgtaaa cttccgggtg gccccaggcc
     4981 caaatcaggt tgatgtacat catcatgttg ccacccatat cgttggtaaa gaaatgggtg
     5041 cccagatagc gatccagggt caacaacgcg acggtaaccg tcagaattgg gaaggaagca
     5101 ataatcagta cgttcgcgca cagtgatgcc caggtaaata ctggcatctt gaacatggtc
     5161 atgcccggtg cgcgcatctt cagaatggta acgaagaagt tgataccggt aagcgtcgta
     5221 cctataccgg atagctggag actccatatc cagtaatcga caccgactcc cggactgtac
     5281 tctattcccg atagcggtgg ataggccagc cagccggtct gcgcaaattc gcccacgccg
     5341 agagaaacgt taaccagaat cacaccaaca acagtaaacc agaagcttaa gttgttgagg
     5401 aacgggaacg caacgtcacg cgcgccgatc tgcagcggaa ccaccaggtt catcagaccg
     5461 ataacgaaag gcatcgccac gaagaagatc ataatcacgc cgtgcgcggt gaagatctga
     5521 tcgtagtggt gaggtggcag gaagcccgct tcgcccgccg aggcaagagc ctgctggcta
     5581 cgcatcataa tggcgtcagc aaaaccacgc agcaacatca caatcgccac gatgatatac
     5641 atgataccga ggcgtttatg gtcgacggag gtcagccact ctttccacag gtaggtccac
     5701 ttaccgaagt aagtgatcag gccaacgagc gccagacctc ccaaaataat gccagcgatc
     5761 gtaaccatga cgataggttc atggaacggg actgcatcaa gtgataattt tccgaacatc
     5821 tttattcttc ctcaacccct ttaatgggcg gattccgcgt ggctcatgtc catgccttcc
     5881 ataccttcgt gtgcactgtg ctcaccttct ggctgggtca tgtccatgct cttaccgtga
     5941 gccataaact tgttaatcac atcggcaaac aagtctggtt tcacgttgga gaaatattcc
     6001 acctggttgt attcgctagg cgcggccagt ttttcgaacg cagccatgtc agacatggtg
     6061 ttcggcgact gtttcgcttt tgcgacccat tggtcgaatt cggcgcgatc cggtgttgca
     6121 atagctttga acttcatgcc tgagaagccc ggcccgctgt agctggcgga gataccgtca
     6181 taagtaccgg gttcgttggc gatcagatgc aggcgagtct gcataccggc catggcataa
     6241 atctggctac ccagacgcgg aatgaagaag gagttcatca cggagttgga ggtcactttg
     6301 aagtacaccg gagtgttcgc cgggaaagcg atttcattca cggtagcaat gccctgttcc
     6361 gggtagatga agaaccattt ccagtccatg gaaaccactt cgatggtaat gggcttctcg
     6421 tcgtgtgcca gcggcttgct aggctcaaga gcgtgagtgg ttttccaggt cagtaccgca
     6481 aggaagatga tgattaagat aggtaccgtc cagaccacag cttccacttt attggagtgt
     6541 gaccagttcg ggctgtactt agcatcttta ttgctcgcac gatacttcca ggcgaaacca
     6601 acagccatca agattgcggg aataacgaca atcaacatca ggccaaatgc cgtcagtatc
     6661 agtgaacgtt gctccagacc aatctgtcct ttgggatcta acagcgcaga attacagcca
     6721 ctgagcaata cagtgcctgc aaataatgac aaccatccca aacttttatt gtatttcctg
     6781 agtctcattt aacgacctca attccacggg atctggtggc gtttaaagtg tgtgggcatt
     6841 ttacgggaag gttacattac tgtaaacatg attaaattcc tgttacttgg atttggctgt
     6901 caggtcacat atgttgcaaa acatatcaag ttgattttaa gaagctgttg caaaagggcg
     6961 ttataacgaa aggggagtgc agaaggagtt tggacgggca attggtataa ccaatctgga
     7021 ataaaacaat tgtttaacaa ataattatca ttatggtgac aattgggtgg gggaaaataa
     7081 agactcatta gctgaatcgt gtaagaaaaa atagaattca taaattgcgt caataatttc
     7141 caaacgctaa gccgcacaaa agaacaaata ttattaagcg atactgatgt ttatttagcc
     7201 gtaataatta cggcgagtga ttactacagc taaataatat ttacagatta cgtcagatgc
     7261 gtttttcgta gcgccagata atccagcaaa ccaccaagca cgacgccaga aagggcgact
     7321 aaaaccccga cttccagcag agcaggcagg aaagagaagt gcgtcaaatc cagcgcgtcc
     7381 atcgtcaaca gcagtaacca cacggccaac aggctgacgc ccgccgccag tgtccacatg
     7441 gcaaaggcat aacctgccgg atattcggta cgggagataa agttgtcatt tactcctgta
     7501 tattcaagcg tctggcggca aaccagcagc aaaataagcc ccggtacggc agcggcgacg
     7561 gagaatagat agaatgtcga ccagccgtgt gcttcaacaa accaacccgc cacggggccg
     7621 acataaactc gtcctacagc ggaaagcgct gagagcaggg caaattgagt tgcggaaaat
     7681 gacttattac acagcgtcat taacagcgcg acaaaggctg atgtgcccat cccgccacag
     7741 aggttttcga aaaagacggc tgcgcccatg ctgtagagat gcttatcagt aatcgacagc
     7801 agccagtaac cagcgttaga cgcaccttgt aaaatgccga aaatcagcag cgcccggaac
     7861 agcgacaggc gctgcatcaa aatcccaccg tacaatgcgc ccacaatggt cgccagtaag
     7921 ccaagcgttt tgttaaccac gcctacttca cccgcatcaa acccgacgcc gcgaatcaaa
     7981 aacgtggttg tcaggctcat ggcgaatgcg tcgcccagct tatacagcac gataagaagc
     8041 aaaataagcc aggcattatt gcgaccaaag aaatctcgca gaggtgcaac aaccgcttgt
     8101 tccagcgttt ttggcacagg aatggtgtcg gtcggttctg gtgcaagcaa cgtcgcgata
     8161 atacaaggga tcaacagcgc cgccatcaac cagtacatgc cctgccagcc cagccattta
     8221 tctgccagcc acagggctaa gccgccggaa accagcatcc ctaaacggta acccagcacg
     8281 ctgattgccg cgcccgcacc acgttcttct gccgggagca catcggtttt ccacgcatcg
     8341 aagacaatat cctgggaggc cgagcaaaaa gcgatcacca ccgccagtgc cgccatccag
     8401 cggagttggg tgccaggttc gagaaaaccc atcgccgcaa tggcgactaa taacaggatt
     8461 tgcgtggcga gcagccaacc gcgccgccgc ccaaaaaatg ggggcgtgta gcggtccatc
     8521 agcggtgacc agaggaattt aaaaacgtaa gcctggccta ccagagagaa gaaaccaatg
     8581 gttttgagat caatattctc gaccgtcatc caggcctgta aggtgccgga tgtcagggcg
     8641 agcggtagcc cggaagcaaa acccaggatc agcaatatgg ctgaacgcgg ctgttgaaaa
     8701 atacgtaaat attgactgga catgggcgtc tacaggcccg gcttgcaccg ggccagaggg
     8761 cagaaattaa cgcgcgttct gcttgatgaa ttcgtgaatg ctggtgtcct gagacatatc
     8821 agcgatggta tcggtcagca cactgttaac cgcatcggcg atatttttgt tggaggcctg
     8881 gaacgcacct tcaacgttgt agctggcacg atagtttttg gtcattttgt tgccattctg
     8941 cgcggtagcg atgatggcga tatccgcttt ggtagcgatg ttgtagcgca cgttgccctg
     9001 ggacacgtca gcatacagtt ggctaacgat gatttgcaga ttaaccgggc cattcggacc
     9061 aaccatgtaa ccacgcgcgg tcatctgttt ttccagcact tcttgcagca ggaaacgcag
     9121 atcgcgggag gcggtcaggg taacgatttg attatcgcgg gtgacttttg ccagcgcctg
     9181 atcggtacgc tgatcggcac cattaatgct tacggtgacg cccatcaggc ttggatcctg
     9241 ctgtggcagt gtaatcgtcg gggaaacttc aatagttgtt ggcggttttg cgcatcctgc
     9301 aagcataaac agagcaacta acgggaagag gatttttttg aacatgttcg ggctctcaga
     9361 gactcttaag cgtgtttggt aaaaattccc gccatcataa cattgccaac ggcgagggga
     9421 agtgggtaag gcatgtaaat tcatcatgtt gacgaaataa tcacccctgg taaaagaaac
     9481 gctgatgcga ggcctgtgtt tcaatcttta aatcagtaaa cttcatacgc ttgacggaaa
     9541 aaccaggacg aaacctaaat atttgttgtt aagctgcaat ggaaacggta aaagcggcta
     9601 gtatttaaag ggatggatga catctcagcg ttgtcggagg agatatttca tgatgatacg
     9661 tgagcggata gaagaaaaat taagggcggc gttccaaccc gtattcctcg aagtagtgga
     9721 tgaaagctat cgtcacaatg tcccagccgg ctctgaaagc cattttaaag ttgtgctggt
     9781 cagcgatcgt tttacgggtg aacgttttct gaatcgtcat cgaatgattt acagtacttt
     9841 agcggaggaa ctctctacta ccgttcatgc gctggctctg catacttaca ctattaagga
     9901 gtgggaaggg ttgcaggaca ccgtctttgc ctctcctccc tgtcgtggag caggaagcat
     9961 cgcgtaaaaa cgcatttgca actgtcggcg cttttccagt atgttgctaa agattttatg
    10021 aaaaacggcc tgcgggccgt tttgttttgt ctggattttg cgctttttgc ccagcattca
    10081 gacgaaaatt gcccgggaat tgtgaaaaaa tacgcgacag cgcgcaataa ccgttctcga
    10141 ctcataaaag tgatgccgct ataatgccgc gtcctatttg aatgctttcg ggatgattct
    10201 ggtaacaggg aatgtgattg attataagaa catcccggtt ccgcgaagcc aacaacctgt
    10261 gcttgcgggg taagagttga ccgagcactg tgattttttg aggtaacaag atgcaagttt
    10321 cagttgaaac cactcaaggc cttggccgcc gtgtaacgat tactatcgct gctgacagca
    10381 tcgagaccgc tgttaaaagc gagctggtca acgttgcgaa aaaagtacgt attgacggct
    10441 tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg ttatggcgcg tctgtacgcc
    10501 aggacgttct gggtgacctg atgagccgta acttcattga cgccatcatt aaagaaaaaa
    10561 tcaatccggc tggcgcaccg acttatgttc cgggcgaata caagctgggt gaagacttca
    10621 cttactctgt agagtttgaa gtttatccgg aagttgaact gcagggtctg gaagcgatcg
    10681 aagttgaaaa accgatcgtt gaagtgaccg acgctgacgt tgacggcatg ctggatactc
    10741 tgcgtaaaca gcaggcgacc tggaaagaaa aagacggcgc tgttgaagca gaagaccgcg
    10801 taaccatcga cttcaccggt tctgtagacg gcgaagagtt cgaaggcggt aaagcgtctg
    10861 atttcgtact ggcgatgggc cagggtcgta tgatcccggg ctttgaagac ggtatcaaag
    10921 gccacaaagc tggcgaagag ttcaccatcg acgtgacctt cccggaagaa taccacgcag
    10981 aaaacctgaa aggtaaagca gcgaaattcg ctatcaacct gaagaaagtt gaagagcgtg
    11041 aactgccgga actgactgca gaattcatca aacgtttcgg cgttgaagat ggttccgtag
    11101 aaggtctgcg cgctgaagtg cgtaaaaaca tggagcgcga gctgaagagc gccatccgta
    11161 accgcgttaa gtctcaggcg atcgaaggtc tggtaaaagc taacgacatc gacgtaccgg
    11221 ctgcgctgat cgacagcgaa atcgacgttc tgcgtcgcca ggctgcacag cgtttcggtg
    11281 gcaacgaaaa acaagctctg gaactgccgc gcgaactgtt cgaagaacag gctaaacgcc
    11341 gcgtagttgt tggcctgctg ctgggcgaag ttatccgcac caacgagctg aaagctgacg
    11401 aagagcgcgt gaaaggcctg atcgaagaga tggcttctgc gtacgaagat ccgaaagaag
    11461 ttatcgagtt ctacagcaaa aacaaagaac tgatggacaa catgcgcaat gttgctctgg
    11521 aagaacaggc tgttgaagct gtactggcga aagcgaaagt gactgaaaaa gaaaccactt
    11581 tcaacgagct gatgaaccag caggcgtaat ttacgcagca taacgcgcta aattcgcaca
    11641 aaggcccgtc accgccaggt ggtgggcttt tttttgtcat gaattttgca tggaaccgtg
    11701 cgaaaagcct ctttcggtgt tagcgtaaca acaaaagatt gttatgcttg aaatatggtg
    11761 atgccgtacc cataacacag ggactagctg ataatccgtc cataaggtta caatcggtac
    11821 agcaggtttt ttcaatttta tccaggagac ggaaatgtca tacagcggcg aacgagataa
    11881 ctttgcaccc catatggcgc tggtgccgat ggtcattgaa cagacctcac gcggtgagcg
    11941 ctcttttgat atctattctc gtctacttaa ggaacgcgtc atttttctga ctggccaggt
    12001 tgaagaccac atggctaacc tgattgtggc gcagatgctg ttcctggaag cggaaaaccc
    12061 agaaaaagat atctatctgt acattaactc cccaggcggg gtgatcactg ccgggatgtc
    12121 tatctatgac accatgcagt ttatcaagcc tgatgtcagc accatctgta tgggccaggc
    12181 ggcctcgatg ggcgctttct tgttgaccgc aggggcaaaa ggtaaacgtt tttgcctgcc
    12241 gaattcgcgc gtgatgattc accaaccgtt gggcggctac cagggccagg cgaccgatat
    12301 cgaaattcat gcccgtgaaa ttctgaaagt taaagggcgc atgaatgaac ttatggcgct
    12361 tcatacgggt caatcattag aacagattga acgtgatacc gagcgcgatc gcttcctttc
    12421 cgcccctgaa gcggtggaat acggtctggt cgattcgatt ctgacccatc gtaattgatg
    12481 ccagaggcgc aactgtgccg ctatacttat ccagggcggc acaacgctgt aagcggcttg
    12541 cgcctgagaa tggcatttgc gtcgtcgtgt gcggcacaaa gaacaaagaa gaggttttga
    12601 cccatgacag ataaacgcaa agatggctca ggcaaattgc tgtattgctc tttttgcggc
    12661 aaaagccagc atgaagtgcg caagctgatt gccggtccat ccgtgtatat ctgcgacgaa
    12721 tgtgttgatt tatgtaacga catcattcgc gaagagatta aagaagttgc accgcatcgt
    12781 gaacgcagtg cgctaccgac gccgcatgaa attcgcaacc acctggacga ttacgttatc
    12841 ggccaggaac aggcgaaaaa agtgctggcg gtcgcggtat acaaccatta caaacgtctg
    12901 cgcaacggcg ataccagcaa tggtgtcgag ttgggcaaaa gtaacattct gctgatcggt
    12961 ccgaccggtt ccggtaaaac gctgctggct gaaacgctgg cgcgcctgct ggacgtcccg
    13021 ttcaccatgg ccgacgcaac cacgctgacc gaagccggtt atgtgggcga agacgttgaa
    13081 aacatcattc agaagctgtt gcagaagtgc gattacgacg tacagaaagc gcagcgcggg
    13141 attgtctaca tcgatgagat cgacaagatt tctcgtaagt cagacaaccc gtccattacc
    13201 cgagacgttt ccggtgaagg cgtacagcag gcactgttga aactgatcga aggtacggta
    13261 gctgctgttc caccgcaagg tgggcgtaaa catccgcagc aggaattctt gcaggttgat
    13321 acctctaaga tcctgtttat ttgtggcggt gcgtttgccg gtctggataa agtgatttcc
    13381 caccgtgtag aaaccggctc cggcattggt tttggcgcga cggtaaaagc gaagtccgac
    13441 aaagcaagcg aaggcgagct gctggcgcag gttgaaccgg aagatctgat caagtttggt
    13501 cttatccctg agtttattgg tcgtctgccg gttgtcgcaa cgttgaatga actgagcgaa
    13561 gaagctctga ttcagatcct caaagagccg aaaaacgccc tgaccaagca gtatcaggcg
    13621 ctgtttaatc tggaaggcgt ggatctggaa ttccgtgacg aggcgctgga tgctatcgct
    13681 aagaaagcga tggcgcgtaa aaccggtgcc cgtggcctgc gttccatcgt agaagccgca
    13741 ctgctcgata ccatgtacga tctgccgtcc atggaagatg tcgaaaaagt ggttatcgac
    13801 gagtcggtaa ttgatggtca aagcaaaccg ttgctgattt atggtaagcc ggaagcgcaa
    13861 caggcatctg gtgaataatt aaccattccc atacaattag ttaaccaaaa aggggggatt
    13921 ttatctcccc tttaattttt cctctattct cggcgttgaa tgtgggggaa acatccccat
    13981 atactgacgt acatgttaat agatggcgtg aagcacagtc gtgtcatctg attacctggc
    14041 ggaaattaaa ctaagagaga gctctatgaa tcctgagcgt tctgaacgca ttgaaatccc
    14101 cgtattgccg ctgcgcgatg tggtggttta tccgcacatg gtcatcccct tatttgtcgg
    14161 gcgggaaaaa tctatccgtt gtctggaagc ggcgatggac catgataaaa aaattatgct
    14221 ggtcgcgcag aaagaagctt caacggatga gccgggtgta aacgatcttt tcaccgtcgg
    14281 gaccgtggcc tctatattgc agatgctgaa actgcctgac ggcaccgtca aagtgctggt
    14341 cgaggggtta cagcgcgcgc gtatttctgc gctctctgac aatggcgaac acttttctgc
    14401 gaaggcggag tatctggagt cgccgaccat tgatgagcgg gaacaggaag tgctggtgcg
    14461 tactgcaatc agccagttcg aaggctacat caagctgaac aaaaaaatcc caccagaagt
    14521 gctgacgtcg ctgaatagca tcgacgatcc ggcgcgtctg gcggatacca ttgctgcaca
    14581 tatgccgctg aaactggctg acaaacagtc cgttctggag atgtccgacg ttaacgaacg
    14641 tctggaatat ctgatggcaa tgatggaatc ggaaatcgat ctgctgcagg ttgagaaacg
    14701 cattcgcaac cgcgttaaaa agcagatgga gaaatcccag cgtgagtact atctgaacga
    14761 gcaaatgaaa gctattcaga aagaactcgg tgaaatggac gacgcgccgg acgaaaacga
    14821 agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa gaggcaaaag agaaagcgga
    14881 agcagagttg cagaagctga aaatgatgtc tccgatgtcg gcagaagcga ccgtagtgcg
    14941 tggttatatc gactggatgg tacaggtacc gtggaatgcg cgcagcaagg tcaaaaaaga
    15001 cctgcgtcag gcgcaggaaa tccttgatac cgaccattat ggtctggagc gcgtgaaaga
    15061 tcgcatcctt gagtaccttg cggttcaaag ccgtgtcaac aaaatcaagg gaccgatcct
    15121 ctgcctggta gggccgccgg gggtaggtaa aacctctctt ggtcagtcca ttgccaaagc
    15181 caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg cgtgatgaag cggaaatccg
    15241 tggtcaccgc cgtacttaca tcggttctat gccgggtaaa ctgatccaga aaatggcgaa
    15301 agtgggcgtg aaaaacccgc tgttcctgct cgatgagatc gacaaaatgt cttctgacat
    15361 gcgaggcgat ccggcctctg cactgcttga agtgctggat ccagagcaga acgtagcgtt
    15421 cagcgaccac tacctggaag tggattacga tctcagcgac gtgatgtttg tcgcgacgtc
    15481 gaactccatg aacattccgg caccgctgct ggatcgtatg gaagtgattc gcctctccgg
    15541 ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac ctgctgccga agcagattga
    15601 acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat agcgccatta tcggcattat
    15661 tcgttactac acccgtgagg cgggcgtgcg tggtctggag cgtgaaatct ccaaactgtg
    15721 ccgcaaagcg gttaagcagt tactgctcga taagtcatta aaacatatcg aaattaacgg
    15781 cgataacctg catgactacc tcggtgttca gcgtttcgac tatggtcgcg ctgataacga
    15841 aaaccgtgtc ggtcaggtaa ccggtctggc gtggacggaa gtgggcggtg acttgctgac
    15901 cattgaaacc gcatgtgttc cgggtaaagg caaactgacc tataccggtt cgctcggcga
    15961 agtgatgcag gagtctattc aggcggcgtt aacggtggtt cgtgcgcgtg cggaaaaact
    16021 ggggatcaac cctgattttt acgaaaaacg tgacatccac gtccacgtac cggaaggtgc
    16081 gacgccgaaa gatggtccga gtgccggtat tgctatgtgc accgcgctgg tttcttgcct
    16141 gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt gagatcactc tgcgtggtca
    16201 ggtactgccg atcggtggtt tgaaagaaaa actcctggca gcgcatcgcg gcgggattaa
    16261 aacagtgcta attccgttcg aaaataaacg cgatctggaa gagattcctg acaacgtaat
    16321 tgccgatctg gacattcatc ctgtgaagcg cattgaggaa gttctgactc tggcgctgca
    16381 aaatgaaccg tctggcatgc aggttgtgac tgcaaaatag tgacctcgcg caaaatgcac
    16441 taataaaaac agggctggca ggctaattcg ggcttgccag cctttttttg tctcgctaag
    16501 ttagatggcg gatcgggctt gcccttatta aggggtgttg taaggggatg gctggcctga
    16561 tataactgct gcgcgttcgt accttgaagg attcaagtgc gatataaatt ataaagagga
    16621 agagaagagt gaataaatct caattgatcg acaagattgc tgcaggggct gatatctcta
    16681 aagctgcggc tggccgtgcg ttagatgcta ttattgcttc cgtaactgaa tctctgaaag
    16741 aaggggatga tgtagcactg gtaggttttg gtacttttgc cgttaaagag cgtgctgccc
    16801 gtactggccg caacccgcag accggtaaag agatcaccat cgctgctgct aaagtaccga
    16861 gcttccgtgc aggtaaagca ctgaaagacg cggtaaacta agcgttgtcc ccagtgggga
    16921 tgtgacgaag ttcaagggcg catctactga tgtgcctttt ttatttgtat tcggtgactt
    16981 tctgtgtctt gtgggctgac aattgccccc gtttcttgtc acaataggcc tttgcgcgca
    17041 tcgatacgtt gcgtgaggta cacagtcatc tacagcggag tgttgttaca ccatgatgga
    17101 cagcttacgc acggctgcaa acagtctcgt gctcaagatt attttcggta tcattatcgt
    17161 gtcgttcata ttgaccggcg tgagtggtta cctgattggc ggaggcaata actacgccgc
    17221 aaaagtgaat gaccaggaaa tcagccgtgg gcagttcgag aacgccttta acagcgagcg
    17281 taatcgcatg cagcaacagc tgggcgatca atactccgag ctggcagcga acgaaggcta
    17341 tatgaaaacc ctgcgtcaac aggtgctgaa tcgtctgatc gacgaggcgc tgttggatca
    17401 gtacgctcgt gagctgaaac tgggtatcag tgatgagcag gttaaacagg cgattttcgc
    17461 gaccccagcc ttccaggttg acggcaaatt tgataacagc ctctataacg gtatcctcaa
    17521 ccagatgggg atgaccgccg atcagtacgc ccaggcgctg cgtaaccagc tcactaccca
    17581 acagctgatt aacggtgttg ccggtaccga ttttatgctg aaaggtgaaa ccgacgagct
    17641 ggcggcactg gtcgcgcaac aacgcgtggt gcgtgaggcg actatcgatg ttaacgcgct
    17701 ggcggcgaag cagcctgtga ccgaacagga gattgccagc tactacgaac aaaacaaaaa
    17761 caatttcatg acgccggaac aattccgcgt gagttacatc aagctggatg ccgcaacgat
    17821 gcagcaaccg gttagcgatg cggatatcca gagctactac gatcagcatc aggatcaatt
    17881 cacccagccg cagcgtaccc gctacagcat catccagacc aaaactgaag atgaagcgaa
    17941 agtggtactt gatgagctga ataaaggcgg tgattttgct gcattagcca aagaaaaatc
    18001 tgccgatatt atctctgccc gtaacggcgg cgatatgggt tggttagaag atgccactat
    18061 ccccgacgaa ctgaaaaatg ctggtctgaa agaaaaaggg caactgtctg gtgtcatcaa
    18121 atcttcggtc ggtttcctga ttgtacgtct ggacgacatt cagccagcga aagtgaaatc
    18181 gttagacgaa gtacgtgacg acattgcggc gaaagtgaaa cacgaaaaag ccctcgatgc
    18241 gtactatgcg ctgcagcaga aagtgagcga tgcggcaagc aacgacacgg agtctctggc
    18301 cggtgcagag caagctgccg gcgttaaagc cactcagacg ggttggttta gcaaagataa
    18361 cctgccggaa gagttgaact tcaagccggt tgccgacgct atctttaacg gcggtctggt
    18421 aggtgaaaac ggcgcgccgg gcatcaactc tgacatcatc accgtagacg gcgaccgcgc
    18481 attcgtgctg cgcatcagcg agcacaaacc ggaagcggtg aaaccgttgg cagatgttca
    18541 ggaacaagtt aaggcactgg ttcagcacaa caaagctgaa caacaggcga aagtggatgc
    18601 tgagaaactg ctggttgatt tgaaagccgg caaaggtgcg gaagctatgc aggctgccgg
    18661 tctgaaattt ggcgagccga aaatcttaag ccgttccggt cgtgacccga ttagccaggc
    18721 ggcgtttaca ctgccactgc cagcgaaaga caaaccgagc tacggtatgg cgaccgatat
    18781 gcaaggtaat gtggttctgc tggcgctgga tgaagtgaaa caaggttcaa tgccggaaga
    18841 tcagaaaaaa gcgatggtgc agggtatcac ccagaacaac gcacaaatcg tctttgaagc
    18901 tctgatgagt aacctgcgta aagaggcgaa aatcaaaatt ggcgatgcgc tggaacagca
    18961 ataatcctga agccgcctcg caaaaaaatg cgttgcagct gtaacaagaa aaggtcgctt
    19021 tcgcggcctt ttccatttct gaacattgcc atttgtttac tgttttcact cccgttaagg
    19081 tgattccact gttaacaaac aaggagaaaa cagtatgaaa cacggaatta aagcactgct
    19141 cattaccctg tccctggcct gtgccggaat gtctcatagc gcgctggcgg cagcttctgt
    19201 ggcgaaaccg acggcggtag aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc
    19261 agtaccggcg aaagccagtg acgaagaagg cacccgggtc agcattaata atgccagcgc
    19321 ggaagagcta gcccgcgcga tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag
    19381 ttatcgcgaa gagtacggtc cgtttaaaac ggtggaggat ctaaagcagg tgccggggat
    19441 gggcaattcg ctggtggaac gtaatctggc ggtattaacc ctgtaattaa tttgcatagt
    19501 ggcaattttt tgccagactg aagaggtcat accagttatg acctctgtac ttataacaac
    19561 aacgtaaggt tattgcgcta tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt
    19621 ttaccagcac gtcaacaacg cccgctacct tgaatttctc gaagaagccc gctgggatgg
    19681 gttggaaaat agcgacagtt ttcagtggat gacggcccat aacatcgcct tcgtcgtggt
    19741 caatatcaat attaactatc gtcgcccagc ggtattaagc gacctgttaa ctattaccag
    19801 tcagttgcag caattaaacg gtaaaagcgg catcttaagc caggtcatta cactggagcc
    19861 ggaagggcag gtggtagcgg atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca
    19921 gaaagcatta gctctggaag gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac
    19981 gttttgtggt gccggatgct caagccgcat ccggcgacac ccggaataat tacctcaacc
    20041 cggttttctg cttcatcgct gccatcaccg tcggtttatc ggccagataa tgattcaaac
    20101 cgttggcgcg taaattacat gccgcacaat gaccgcaacc gtcgccttta aagccgttat
    20161 agcaggtcaa cgtttcgtta cggactaaat ccagtttgcc gtaataatct gccagcgccc
    20221 aggtttccgc tttatcaatc cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc
    20281 ccaaactgac ggcatggttt agtgctttca caaactcatc gcggcaatcc gggtagccgg
    20341 agaaatccgt ttcacagacg ccggtaatta cggcttctgc ttttacctga tacgcatata
    20401 ttgccgccag cgtcaggaac aaaatattac gcccagggac aaacgtattc gggatgccat
    20461 cggcttcagg ttcataatca ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca
    20521 gctcgttgag cagcgttaca tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca
    20581 gttcgcgtgc cacgtcgatt tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat
    20641 ggacttcatc atattgttgt aatgcctgca ccagacaggt ggtggaatcc tggcctccac
    20701 tgaacacaac gacagcacgt ttcatagata atcctgcttg aacaataaaa gcgttatggt
    20761 aacgcctgcg attaacccgg accagctatt cattgcgacg gcgcgggaag ccaggcttcg
    20821 gtaaattcaa accagccgcg cggtgtcagt cgcacaccgt tcacgccggg aggggcacta
    20881 atgcgatagt gatagttgaa cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa
    20941 accgctttca gggcattaaa tcggttttcc tcatcaggca ttacttgcac cgcgtccagc
    21001 gtcgattgca gatgtgcata tgctggagcg tcgaaaacat gtggccatag tggatcgcaa
    21061 cgtagccatt gctccagagt atattccggt gcttcgccaa ttaatctgtc gcccatcatg
    21121 aggtctgcgt gtgccagtag ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt
    21181 gtgagttcac agccttccgc tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt
    21241 tctatcggta ggtgataaac cagcgtcaag gttttcggta gtttgacttc atccggtact
    21301 tgccagtacg gaatagtcca gcctggcagt aatgcatgac tggcggtgat caggttttct
    21361 ccgacttcta acgtttgtaa taaaccggat tgatgaataa tggagatcac ttttcgcgcc
    21421 tgccagaggg agagtcgggg acttttgcgc aacgttaaat agcaaaaacc taaactgatg
    21481 ccactactga cctggctgac ccgttgcagc tcctccggtt tgccgatggt gatttgcacg
    21541 ggatgccgac aactgtttcc caaatctttt tcgaaaagcg gcggagttat ccagtactca
    21601 accgctttaa gcagcggatg acgcaaatgg taataatcat ggctctccag gcgcaccagc
    21661 tccgctgtga attgtgttaa gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc
    21721 gccagatggc tgcaatagct cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa
    21781 aagaaggtca gacactgcgg atgggtgact tcaatatgct tcacgctaat aaataattga
    21841 tccagtgctg gcagttgtaa cagcatcaat aatcgctggt gtaagtgtga ggcttttact
    21901 gcatcgccgt tatgccagtg tagggttgaa cgaagataaa agtcccagcg taacccgtca
    21961 gtagaggttt cccagtgatg cgctaaatcg ccaatcgggc gctgagtatt attatcgaag
    22021 cgggtcaggc cggaaaatat ctgcccggcg agatgctgct cggcacggcc gggcaaaaag
    22081 cctggttgta gcggttcgag cgggcgatag tagggaatac gcaacgtggg tgtatcgttt
    22141 tgccattgtc cgcccataaa cggctgtaac agagtgcgca gctcacctgg ggccagttgc
    22201 gccagctcca gcacatcttg ctgctttccg gtttccagtg cctgttccat catcgcattg
    22261 cgtagcgatt ccggcgtgac cagaaagcgt aattgtccgc gctttccgcg tcctgactgc
    22321 gcctgccact ccagccatcc cgcctcctgt gcctgacgca acagcgtacg aacatggcgt
    22381 tcgctgcaaa aacagcgttc ggccagttcg ctgacggtga cggtttgcgg ctttccggct
    22441 gaaggttgcc acagacgttg atactggtta agacggttga gcaatcgcat ataaacccgg
    22501 aacaatatta tttaactatt cactattact tccgtatata tcaggtgata ctcaatcacc
    22561 attaaccgtg tcacagagtg gagaaagaaa tggctcgtct ggcagcattt gatatggatg
    22621 gcactttatt gatgcctgac catcatttag gtgagaaaac cctctctact ttggcgcgac
    22681 tgcgtgaacg cgacattacc ctcacttttg ccacggggcg tcatgcgctg gagatgcagc
    22741 atattctcgg ggcgctatcg ctggatgcat atttgattac cggcaacgga acgcgcgtgc
    22801 attctctgga aggtgaactt ttacatcgtg atgatttacc tgcggatgtc gcggagctgg
    22861 tgctgtatca gcaatgggat acccgagcca gcatgcatat cttcaatgac gacggttggt
    22921 ttaccgggaa agagatccct gcgttgttgc aggcatttgt ctatagcggt tttcgttatc
    22981 agataatcga tgtcaaaaaa atgccactcg gcagagtcac caagatctgc ttctgtggcg
    23041 gtcacgacga tcttacacgc ttgcagatcc agctatacga agcattaggc gagcgtgcac
    23101 atttgtgttt ttccgccacg gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg
    23161 ctgcattgac ggtgctgacc caacatttag gtttatcgtt gcgcgattgc atggcctttg
    23221 gtgatgcgat gaacgatcgc gaaatgttag gcagcgtcgg tagcgggttt attatgggca
    23281 atgcgatgcc gcaactgcgc gcggagctcc cgcatttacc ggtgattgga cattgccgaa
    23341 atcaggctgt ctctcactat ttgacgcact ggctggacta tccacatcta ccttattccc
    23401 ccgaataacg agatcccttc cagcaccggg caattgcccg gttttttttg cgttgaattt
    23461 gtcattttgt gccgtggtgt ttaaaccgca cagaataaat tgtcgtgatt tcacctttaa
    23521 aatagaatta aaagagaaaa aaattctctg tggaagggct atgttagata aaattgaccg
    23581 taagctgctg gccttactgc agcaggattg caccctctct ttgcaggcac tggctgaagc
    23641 cgttaatctg acaaccaccc cttgctggaa gcgtctgaaa cggctggagg acgacggtat
    23701 ccttatcggc aaagtcgccc tgctggatcc ggaaaaaata ggcctcggcc tgaccgcttt
    23761 tgtgctgata aaaacgcaac atcacagcag cgaatggtat tgccgctttg tcacggtggt
    23821 taccgaaatg ccagaagtgc tggggttctg gcgcatggct ggtgaatacg attatctgat
    23881 gcgcgtccag gttgccgaca tgaaacgcta cgacgagttt tataagcgtc tggtaaacag
    23941 cgtgccgggg ctgtcggacg tcacttccag cttcgcgatg gaacagatta aatacaccac
    24001 ttctttaccc atcgaataaa tatccagaat caggtcagga cacaacgcgt gcgattattt
    24061 gctcaattaa gctggtattt ccgtcgggaa tggcgtcgct atctcggggc tgtcgccttg
    24121 cttgtcatta tcgcgatgct gcaactggtt ccgccaaaag tggttggtat tgttgtcgat
    24181 ggcgtgacag aacaacactt tactaccggg ctgatcctga tgtggatcgc caccatggtg
    24241 ctgattgccg ttgtggttta tctcctgcgt tatgtctggc gcgtattgct gtttggcgcg
    24301 tcttaccaac tggctgttga actgcgtgaa gattattacc gtcagctaag ccggcagcac
    24361 ccggagtttt acctgcgtca tcgcacgggt gatctcatgg ctcgtgcgac caatgacgtc
    24421 gatcgcgtcg tgtttgccgc cggagaaggg gtgctgacgc tggtggattc actggtaatg
    24481 ggctgcgctg tgttgattat gatgtctacg caaattagct ggcagttgac cttatttgcc
    24541 ctgttaccga tgccagtgat ggcgatcatg attaagcgca acggcgatgc cttgcatgaa
    24601 cgctttaagc tggcacaggc ggcgttttcc agtcttaatg accgcaccca ggaaagcctc
    24661 accagtatcc gcatgatcaa agcctttggt ctggaagatc gccagtcggc gttatttgcc
    24721 gcggatgccg aagataccgg caaaaaaaac atgcgggtgg cgcgtattga tgctcgtttc
    24781 gacccgacaa tctatatcgc gattggtatg gcgaacttgc tggcgattgg cggcggtagt
    24841 tggatggtgg tgcagggcag tttaacgctg ggccagctca ccagttttat gatgtattta
    24901 ggtctgatga tttggccaat gctggcgctg gcatggatgt ttaacattgt ggaacgtggt
    24961 agtgctgcgt acagccgtat tcgtgcgatg ctggcggaag cgccggtggt gaacgatggt
    25021 agcgaacccg tgccggaagg gcgtggcgaa cttgatgtaa atattcacca gttcacgtat
    25081 ccgcagactg accatcgtgc gctggaaaac gtcaatttcg ccctgaaacc cggtcagatg
    25141 ctgggtatct gcgggccgac tggttccggc aaaagtaccc tgttgtcgct cattcagcgt
    25201 catttcgacg tcagcgaggg ggatattcgc tttcatgata ttcctctgac gaagttacaa
    25261 ctcgatagct ggcgtagccg cctggcggta gttagccaga caccgttcct tttttccgac
    25321 accgtagcga gcaacatcgc gttgggttgc ccgaatgcca cccagcaaga gattgagcat
    25381 gtcgcgcggt tagccagcgt acatgacgat attttgcgtt taccgcaagg ttacgataca
    25441 gaggtgggcg agcgcggcgt gatgctttcc ggcgggcaaa aacagcgtat ctctattgct
    25501 cgtgcgttat tagtcaacgc ggaaatcctc atccttgatg atgcgctttc ggcggtggac
    25561 ggacgcacag agcaccagat cctgcataac ctgcgtcagt ggggacaggg tagaacggta
    25621 atcatcagtg cccatcgcct ttcggcactg acggaagcca gtgaaattat tgtgatgcag
    25681 cacggacata tcgcccagcg tggcaatcat gatgagctgg cgcaacaaag cggctggtat
    25741 cgcgatatgt atcgctatca acaactggag gcggcgctcg acgacgctcc ggaaattcgc
    25801 gaggaggccg tcgatgcgta gttttagcca actgtggccg actctcaagc gtctgttagc
    25861 gtacggttcg ccgtggcgta aaccgctggg gattgcggtc ctgatgatgt gggttgcggc
    25921 agcggcagaa gtcagtgggc cgctgcttat cagctatttt atcgacaata tggtagcgaa
    25981 aaataacctg ccgttgaaaa tggttgcagg gctggctgcg gcgtatgttg ggctacaact
    26041 gtttgccgcc gggctacatt acgcgcagtc gctgctgttt aatcgggcgg cagtaggcgt
    26101 agtgcaacag ttgcgtaccg acgtgatgga tgccgcattg cgccagccat taagcgagtt
    26161 tgatacccaa cccgtcgggc aggtgatttc ccgcgtcact aatgacactg aagtgatccg
    26221 cgatctctac gttaccgtag tggcaactgt cctgcgtagc gccgcactgg tgggcgcgat
    26281 gctggtggcg atgttcagcc tcgactggcg aatggcgctg gtggcgataa tgattttccc
    26341 ggtggtgctg gtggtaatgg tgatatacca gcgttacagc acgccgattg tccgtcgtgt
    26401 gcgcgcctat ctagcggata tcaacgacgg ctttaacgaa atcatcaatg gcatgagcgt
    26461 tatccagcag ttccgtcagc aggcgcgatt tggcgaacgt atgggggagg ccagtcgttc
    26521 acactatatg gccaggatgc aaaccctgcg cctcgacggt tttctgctgc gtccgctgct
    26581 gagtctgttt tcatcgctca ttctttgtgg cttgttgatg ctgtttggct tctccgctag
    26641 cggcaccatt gaagtgggcg tgctgtatgc gtttatcagc tatcttgggc gacttaacga
    26701 accattaatc gaactgacca cgcaacaggc gatgctgcaa caggctgttg ttgctggtga
    26761 gcgcgtgttt gaactgatgg acggaccgcg ccagcaatat ggcaatgatg atcgcccgtt
    26821 acagagtggc accatcgaag tcgataacgt gtcatttgct tatcgcgatg acaatctggt
    26881 gctaaagaac attaatctct ctgtgccttc gcgtaacttt gtggcactgg tcgggcatac
    26941 cggcagtggc aaaagcaccc tcgccagttt attgatgggc tattacccgc taacggaagg
    27001 tgagattcgc cttgatggtc gtccattaag ttcgctaagt cacagcgcgc tgcgccaggg
    27061 cgtggcaatg gtgcagcaag atccggtggt gctggcggat accttcctcg ccaacgtgac
    27121 gctggggcgt gatatctccg aagaacgcgt ctggcaggcg ctggaaaccg tgcaactggc
    27181 ggagctggcg cgcagcatga gtgatggtat ttatacgccg ctgggcgagc aggggaataa
    27241 tctctccgtc gggcaaaagc aactgctggc actggcacgc gtgttggtgg agacgccgca
    27301 aatcctgatc cttgatgagg caaccgccag cattgactcc ggtactgaac aggcgattca
    27361 acatgctctg gcggctgtgc gtgaacatac cacgctggtg gtgattgctc accgcttatc
    27421 gaccattgtt gatgccgaca ccattctggt gcttcatcgt gggcaagccg tggagcaggg
    27481 cactcaccag caactgctgg cggcccaggg acgctactgg cagatgtatc aactgcaact
    27541 tgcgggcgaa gagctggcag ccagcgtgcg tgaagaagaa tcattgagcg cctgaatagc
    27601 gcaatatttc atcgttggtg caaaaatgta acgcactgtg cactgtcata gtgcgttttc
    27661 attttcaatc ttcttaactt cctgctctct ttctcgtttt tcatttctgg cacaccgctt
    27721 gcaatacctt cttcgtgtag cagaaccatt accgaattct gaccggaggg gatctatgaa
    27781 gctggtgacc gtgataatca aaccattcaa gctggaagac gttcgtgaag cgttatcttc
    27841 cattggtatt cagggcctga ccgtcaccga agtgaaaggt ttcgggcgtc agaaagggca
    27901 tgccgagctg taccgggggg cggaatacag cgtcaacttc ctgccaaaag taaaaattga
    27961 tgtggcgatt gctgatgacc aactcgatga agtgatcgat atcgtcagta aggcggctta
    28021 caccggaaaa attggcgacg gcaaaatctt cgtcgctgaa ttgcaacgcg tcattcgtat
    28081 tcgtaccggc gaagccgacg aagcggcgct gtaatctctg gcacacagca acaggaacga
    28141 aaaatgaaga tagcgacgat aaaaactggg cttgcttcac tggcgatgct gccgggactg
    28201 gtaatggctg cacctgcggt ggccgataaa gccgacaatg cgtttatgat gatttgtact
    28261 gcgctggtgc tgtttatgac tattccgggg attgccctgt tttacggtgg gttgattcgc
    28321 ggcaaaaacg tgctgtcgat gctgacgcag gtgacggtga cgtttgcact ggtctgtatt
    28381 ctctgggggg tttacggtta ctcgctggcg tttggtgagg gcaacaactt cttcggcaac
    28441 attaactggt tgatgctaaa aaacatcgaa ctgacggcgg tgatgggcag catttatcag
    28501 tatatccacg tggcgtttca gggctcgttt gcctgcatta ccgtcggctt gatcgttggg
    28561 gcgctggcgg aacgaatccg cttctcagct gtgttgattt tcgtggtggt atggctgacg
    28621 ctctcttaca ttccgattgc gcatatggtg tggggcggtg gtttgctggc ttctcacggt
    28681 gcgctggatt tcgcgggtgg cacggtggtg catattaacg ccgcaatcgc cggtctggtg
    28741 ggcgcgtatc tgataggcaa acgcgtgggc ttcggcaaag aggcgtttaa accgcacaac
    28801 ctgccaatgg tcttcaccgg tactgccatt ctctatattg gttggtttgg ctttaacgcc
    28861 gggtcagcgg gcacggcgaa tgaaatcgcg gcactggcat ttgtgaatac tgtggtcgca
    28921 acggcggcgg caattcttgg ctggatcttc ggtgaatggg cgctgcgtgg taagccttct
    28981 ctgctgggag cgtgttctgg cgcgattgcc ggtctggtcg gcgtgacgcc agcgtgtggc
    29041 tacattgggg ttggcggcgc gttgattatc ggcgtggtag ctggtctggc gggcttgtgg
    29101 ggcgttacta tgctcaaacg cttgctgcgg gtggatgatc cctgcgatgt cttcggtgtg
    29161 cacggcgttt gtggcattgt cggctgtatc atgactggga tttttgccgc cagttcgctg
    29221 ggtggcgtgg gcttcgctga aggtgtgacg atgggccatc agttgctggt acagctggaa
    29281 agcatcgcca ttacgatcgt ctggtccggt gtggtggcat ttattggcta caaactggcg
    29341 gatctgacgg ttggtctgcg tgtaccggaa gagcaggagc gagaagggct ggatgtcaac
    29401 agtcacggcg agaatgccta taacgcgtaa caagcactgc aaaaaatagc cggacggttt
    29461 tacctccggc tattttttta attgtgatta cgcatcaccc cttcctgaac ggtcgaggca
    29521 accagcacgc cgtcctgggt ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc
    29581 gaggtgctct ccacgctata cagcagccat tcattcaaat taaacgggcg atggaaccac
    29641 atggaatggt caatggtggc aatctgaatc cccggttcga gaaaaccgat gccgtgcggc
    29701 tgtagagcta ccggcaggaa gttaagatca gaagcgtaac cgagcagata ctgatgaacg
    29761 cgcaggtcat ccggcacgct accatttgcg cggatccaca cctgacgatg tggttctgcg
    29821 acgtgacctt tcagtgggtt atgaaactcc accggacgga cttccagcgg acgatcgcag
    29881 atgaatttat ctttaagcac tggcggcagc aggtgcgcca gcgattgggc gatttgcgtt
    29941 tccgaaggca ggccatcagg cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc
    30001 gcttctggtg cctggaaaga ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca
    30061 gcaacccggc gggcgctgaa gctgttaccg tcacgcagcg tttcgacatc ataaataatc
    30121 ggcttcttac tatcgccagg gcgaagaaag tagctgtgaa acgaatgtac cagccgctct
    30181 tcagggacgg tctcttttgc agcatacaag gcctgaccca cgacctggcc gccaaacacc
    30241 tggcgtaaac ctaaatcttc actctggccg cgaaagagtc cttcctcaat tttttccaga
    30301 tttaacaatg tcagtaaatt ttttagcgcc tgactcatat aactctccag taacaaagct
    30361 gccgcagcaa gccaaagtga gttgagtata acgcaaattt gctactggtc cgatgggtgc
    30421 aatggtctga attacgggct aattacaggc agaaatgcgt gatgtgtgcc acacttgttg
    30481 acgttactat tttgttaacc actcttccgg cgaggaaagt tagcccgctg gtgcattgat
    30541 aataaggaga aatgaatgaa actcgtgcac atggccagtg gtttagcggt tgcgattgcg
    30601 ttggcggctt gcgcagataa aagcgcggat attcagacgc cagccccggc tgcaaataca
    30661 tctatttcag caacacaaca acctgctatc cagcaaccga atgtctccgg taccgtctgg
    30721 atccggcaga aagtcgcact gccgcctgat gctgtgctga ccgtgacact ttctgacgcg
    30781 tcgttagccg atgcaccgtc aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa
    30841 cagtcaccat tcagctttgt tctgccattt aatccggcag atgttcagcc gaacgcgcgt
    30901 attctgttga gtgcggcgat taccgtgaat gacaaactgg tatttattac cgataccgtt
    30961 cagccggtga tcaacaaggg cggaactaaa gccgacctga cattggtgcc ggtgcagcaa
    31021 accgccgtgc cggttcaggc cagcggtggt gcaacgacta ccgtaccttc gacttcacca
    31081 actcaggtga atccgtcttc ggcagttcca gctcctacgc aatattaagc gacgttaacc
    31141 ctctccgccc ggagagggtt agtagttcca gcgataacgc tgcaagtcga tttgcccgct
    31201 tcccgatacc atcacacctt ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc
    31261 ggttagcgaa attgtgccgt ggcgattaac cacccggtgc cagggtaagg tgctgccttc
    31321 agggagacgc tttaacacac cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt
    31381 cgccacatca ccgtaagtgg tgacatagcc ttcgggaata gcggcgacga tttgccagac
    31441 gcgttgggga aatgaatctt ctttttccat cttttcttcc tgaggtaatt tttcagcata
    31501 atctggaaaa acgcccgagt gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat
    31561 gggttgcaat tagccggggc agcagtgata atgcgcctgc gcgttggttc tcaacgctct
    31621 caatgggggc tctgttggtt ctcccgcaac gctactctgt ttaccaggtc aggtccggaa
    31681 ggaagcagcc aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc ccccacccat
    31741 ttctgcctcc caccgtttcg tcaaaaaacc ccaacatggc taaactttaa ccacaactga
    31801 cgtcgcaaga attgtctggc tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt
    31861 gatggttttg tggttgccgt tcctgccgat aaaaaggatg cctatcggga aatggccgct
    31921 aaggctgcgc cattgtttaa agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc
    31981 gatgtaccgg atggcaaagt gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa
    32041 gaggttgtct ttagctggat tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa
    32101 aagatgatgt cggacccacg gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag
    32161 cgaatgatct atggcggatt cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg
    32221 cggcgtgccg tcagcccaaa aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct
    32281 ttcggtaatg ccttgctgta gagccagccc tgaccgtagt gcacgccatg ctggcgtagc
    32341 cactcttctt gtttactggt ttcgattccc tccgctacca ttttcagttt cagtgttttt
    32401 gccatttcga tgatatgcgg cgtgacattt ttatattcca gcgcatcaac gaaagattta
    32461 tcgatcttca gaatgtcgac atccagatcc tgtaaataac ttaaacttga atatcccgta
    32521 ccaaaatcat caagataaat ttcatggccc gcctcccggt agcgagaaat tatcggggcg
    32581 ctggttttcg gatcggcaaa ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta
    32641 acctgatagt gattgatcat ttcacgcagc aattgcggga ttttttccga ggtgagcacg
    32701 gtggattcaa gattgatcga aatatgctgc tgtggatgct gacgcagcca gtcgcccata
    32761 tcttcaaaga cgcttcttat aatcagtagc gtcaatggct cagaaaggcc cgtttgctgt
    32821 gccagcggaa taaaactatc tggtgacaac caactaccgt ctgtctgcgg ccagcgcgcc
    32881 agtgcctcag caccgacaat tttgccattg gctaaggaga caatcggctg atagtgcacg
    32941 caaatatcac gattttcgat agcatcctgc agccgatgat gcggtgactg gatacggcgc
    33001 aaaatacgca gcacaaacat cgctgccagc aggccaatca ccaacccgag cggtaaccaa
    33061 ataaagactt gccgatgcca gcctttctgc aacattttcg ttgaagccca ggtgatgatc
    33121 gaaatattca tctccgggaa gggcaggata tcgtaaatga ttccattatt ttcgatatgc
    33181 tcaccgggtg ttttttgtag cctggtaata attccctgag caatttcatc gctgctggtt
    33241 atgacaacgt tatgggcatt gccaataatg gcggcatcaa tttgccatga gctataggga
    33301 atgacatcaa taaaggaagc ggggtcgatc atgacgacat aatgtgccgt tcccatggcg
    33361 accatgtaac ggataatgcc taaatcgtta tgcgatgtta accagacacg ataaccatct
    33421 ttcgaaattt tacctggctc ggggaaggta tcgggcggac tttcatgctc cagagacgaa
    33481 cactggggaa cgttgttatc gatataaacc acttcctgaa tatagcgata actgtaagat
    33541 accagacgca tttccatgag atgggcttcg ctacaggcag cgccttgcca tctttccagc
    33601 tcttgcagcg catctttccc ttgtgtcgcc accttattgg cccgaatagc gacgcgggag
    33661 gaataggtat ccagctcttc aataaacgat gtttctacct gctgatgggc cagccagatg
    33721 cttaagccga caggcagcaa tactgaaaga atcagtactc ccgaaataag gccgaccaga
    33781 tgtcgtgttc tcaccagcat atccttatta acacagtgta gggaaagtat atctgattgt
    33841 ggaaaaaggc tggcaggaga taaggaggta gagctggtat ttcatgaagt gatgggtgga
    33901 aacgatcatt gtcatttgac caggcgcaga gctttacgcc tggtatgtaa ccctaactta
    33961 agatgttgat tcggcgcgag cgagggtaaa aatatcgtaa aaagacagtt cacctttacg
    34021 gacgatcatt ttttgcagtt gttccttatg aacatccgcg acacgcggag agttcaagat
    34081 ggcttcaacc agcgcggcag gtacaaagcg cgtgttgtac cattcgccgt tatagcaaac
    34141 gcggaaatcc agcacgtcga tatcttcata gcggtagcgt gggtagacat cgaaaaagaa
    34201 gaaaccatta agcaggataa ataatacaac cagtagccag acagagcctg acagcgtttc
    34261 ggactgcaac atcaccgcca gggcggcaaa aaaagcaacg tacataccga taaacagccc
    34321 cggatgttta cggataaaac tgatactaaa gcggggtttg ttgtcgcgtt tttcgcgggt
    34381 attcagagac tcaatcgttt cggtcagcag gcgttgtatt tcggtcatcg tttgccttcg
    34441 tggtatctgc aactttccag ggaacaccct attttaacgt gggaatagta gaggggaagt
    34501 aacagatcta ccacaatttt gcataacagt tgcgaaaaac cgattacaat tttttaatta
    34561 ttctggctgg attaccgccc acgacaacgt tgtccgggac atcttttgtg acaactgcac
    34621 ctgaggctac cacgacgtta tcaccaatgg tcacaccagg gttaatgacc gcgcgtccgc
    34681 caatccagac gttattaccg atggtgacgg gtttccccag ttcagcaccg ctattacgtg
    34741 ctacagggtc gatgggatgt gttgccgtgt agatatgaac gcctggtgcc aacatacagt
    34801 tatcaccgat gcgaataggg cagacatcaa gcatcacgca atcgaagttg gcgaaaaaat
    34861 tattaccgag aaaaatgtta tagccatagt cacagcgaaa cgttggctca atataagcct
    34921 ctgtcacctg accgaataga tcagcgagaa tttgctggcg taatgtgtgc tcttccgcca
    34981 gggaatgatt gtatcggtga ataagctgac gagcgcgcag gcgatcgcga gataacgtct
    35041 catctgccga gcgatacaac tcaccagcaa tcatcttttc tttttctgtg ctcattactg
    35101 acctccggtt gctgaaaatg cggcaacaca gtaagacgaa aagtcagtga agagaacgtt
    35161 cccattacat ctgtgtgata aagatcacat agggtatgtc ttcatggtga aaaagtataa
    35221 aattcttaat aaacagccgg ttatagctcc gaaagcgaat tatttattag cgaataaatt
    35281 tccatactga ggaagggatc ttgtcgtata gtttattcat ggtcaattcg gcgaggcggt
    35341 gatctgcggc tgagtaaaat accgccagtt cattatctga taattcgtat ttatttttct
    35401 cgataacacg ctccagcgtg tcaattgtct ggcaacgacg taaacgcatt aaataatcgg
    35461 ttttcgttaa aggtttttcg gacatacttc tacccatggt tgtaataatt ctaacaagat
    35521 aaactcgcag gattctcttt cgtcgcattg acaaaacaac ggaatagtcg attacctgac
    35581 ttccgccatt tctgaagatc ctgcatatta ataccataac tgctgaacaa cataaaggtg
    35641 tcatccagat attcgtcgat ctgctcaatg agcttattgt cttcattata cttaattttg
    35701 taattaagtg cgaaggtcgc aatatgctca atcagttcat tcaactggag gttgatcgcc
    35761 gaggttgggt cgttaaccca gccatgattg ctttcttcaa ggtttgcaag acagtcatga
    35821 tacagggttt cacagagaaa cttaagctgt gcgatatcat gtcttttggg tgagtattca
    35881 tccataacgc gtccccttct tagtggttga actaacggac acctttcggg atggaaaaaa
    35941 cttactgacc tggacttgcc ctctcgtttg ttagcttaac tataagccac tctttgcagg
    36001 ttttcatcgc attattacga aaaattacaa ttaggaagga atcgggacag gactcacttt
    36061 ttcatactag cactaacccg cagcaggtaa aagcagtttg ctcgaactgt tcataacatt
    36121 tcaggattat cgccacatga aattggtgac actaatacca ggattgctct gaatatgacg
    36181 taataaccga ggaatgaata aagaattacc gcaaataatt aagaatagcc tctaaatgat
    36241 tatggattat taaatctatt agcgctactt aatataaatt aactaacaat cagtattttt
    36301 tatgaatttt atccgtggtt aatactggtt ttcgtatgag atcctgagtt ggtggttcaa
    36361 ttactcctta atgttcgtag gttatgcata aaaaaggccg cttgcgcggc cttagtgatt
    36421 acacgttgta tcaatgatga tcgacagtat ggctgtgctc gatatcttca ttcttgcggc
    36481 taaagcggcg gcgaaccacc acaaagaata ccggaacgaa gaagattgcc agtaccgttg
    36541 cggtcaccat cccgcccatt acaccggtac ctactgcgtt ctgcgcgccg gaaccagcac
    36601 cagtactgat aaccagcggc ataacgccga ggataaacgc cagcgaggtc atcaggatcg
    36661 gacgtaaacg catccgcact gcatcaagcg tcgcttcaat cagaccttta ccttctttat
    36721 ccatcaagtc tttggcgaat tcgacgataa gtatcgcgtt cttcgccgac aacccaatgg
    36781 ttgtgagcag gcctacctgg aagtaaacgt cattggtcag gccacggaag gtggcagcca
    36841 gcaacgcacc gataaccccc agcggaacga ccagcataac ggagaacgga atcgaccagc
    36901 tctcgtacag cgccgccaga cacaggaata cgacaatcaa cgaaatcgcg tacagtgaag
    36961 gtgcctggtt gccggagagg cgttcctgat aggacatccc cgtccagtca tagccaacac
    37021 cggtaggcag tttgctcgcc agttgttcca tcagctccat tgcttcaccg gtacttttac
    37081 ccggtgccgc ctggcctaag atttccatgg atggcaggcc attgtaacgt tccagacgcg
    37141 gcgaaccgta ctcccaacga gaagaggaga atgccgagaa cggcaccatc tgaccatcag
    37201 cagcacgaac ataccagtcg ccgatatcat ccggcagcat acggtatttc gcttctgaca
    37261 tgacgtaaac tttcttcaca cgaccgcggt cgataaagtc gttcacatag ctaccgcccc
    37321 atgcagcgcc cagagtggtg ttaatgtcat tgatagaaac acccagcgcc tgcgcttttt
    37381 cccggtcgat atcaatctta aactgcgggg tatcttccag accgtttgga cgtacgctgg
    37441 tcaacatatc agggtgcttc gctgcttctg caagcaactg gttacgcgcc tgagtcagtt
    37501 tttcgtgacc aaggccagcc tggtcaatca gctcaaagtc aaagccggtt gcagtaccca
    37561 gttccacgat tgcgggcagg ttaaaggcga aaaccatcgc atctttgatt tgcgagaaag
    37621 cgcgtgttgc acgcatggta atcgcttcaa ctttgttttc ttcgcccgga cgatcggccc
    37681 agtccttcaa ggaaacgaac gcaataccgg tattctgacc acgtcccgca aagccgaagc
    37741 cgttaacggc gaacaccgac tcaacgttgt tcttttcttt ggtcagatag taatgcgtta
    37801 cctcattgag cactttctgc gtacgttcct gcgttgcacc tgctggcagc tgaaccatgg
    37861 tcataaatac gccctggtcc tcatctggca agaaggagct tggcagacgc acgaacagat
    37921 aggccatgcc gaccacgatg atcagataca gcaccaggta acgccccgta ctgcgcagaa
    37981 taccgcctac gctgtcggtg tagtggtgcg tgctcttctc gaacatgcgg ttaaaccagc
    38041 cgaagaagcc ttttttacct tccccgtgat cgcctttggc aatcggtttc agcatggtgg
    38101 cacaaagagc cggagtcagg atcaacgcta ccagtaccga cagcgccatt gctgaaacaa
    38161 tggtaataga gaactgacga tagatagcac cagtagaacc gccaaagaag gccatcggta
    38221 cgaataccgc cgacagtacc atcgcgatac cgaccagagc gccctgaatc tgccccatcg
    38281 acttacgggt agcttctttt ggcggcaaac cttcttccgc cataacacgc tccacgtttt
    38341 ctaccacaac gatagcgtca tccaccaaca agccgatggc gagcaccatc ccgaacattg
    38401 ttagcgtgtt tatcgagaag ccaaaggcgg caaggacggc aaaggtcccg agcaatacca
    38461 ccggtacggc aatggtcgga atcaacgtcg cgcggaagtt ctgcaggaac agatacataa
    38521 ccaggaacac gaggatgatc gcttcgacca gcgttttaac cacttcgtga atagagattt
    38581 tcacgaacgg cgtggtgtcg tacgggtaaa caattttcag acccgacggg aagaacggtt
    38641 ccatcttcgc cagttcagca cggattgccg cagcggtatc cagcgcgttt gcaccggtcg
    38701 ccagcttgat ccccagaccg gaagccggtt ggccgttaaa ctctgcgatg atgtcgtagt
    38761 tctcaccacc cagctcaatc ttcgccacat cacgcagcag tacgcgggaa ccatcctgat
    38821 tcactttcag caggattttg ccgaactctt cagtagaggt cagacgcgtc tgagcaataa
    38881 tagaggcgtt aagctgttgg cctttcaccg gcggcgtacc accgagctga cccgccgcaa
    38941 cctgggcgtt ctgcgctttg atggcggtaa tgacatcaac cggcgttagc tggaatttgt
    39001 tcagctcatt cgggttcatc cagatacgca tcgcgtactg tgaaccgaac aactgaacat
    39061 cacccacgcc cgacgtacgg ctgatggcat ctttcatatt cgccgctacg tagtcagaga
    39121 tatcctcctg cgtcatggtg ccatcggtgt tgataacgcc gacaaccatc aggaagctgc
    39181 tggatgattt ctcaacgctc accccttgct gctgaacttc ttgcggcagc aacggcatcg
    39241 ccagctgcag tttgttctgc acctgaacct gcgcgatatc cgcatcagta ccagactcaa
    39301 aggtcagggt gatctgcacg gtacccgtgg agtcactgtt agaggacatg tacatcaggt
    39361 tatcgatacc gttcatattc tgttcgataa cctgtgtcac cgtgtcctgc actgttttcg
    39421 catcagcgcc ggggtaggag gcggagatcg ttactgccgg cggtgcaatc gtaggatatt
    39481 gcgccaccgg cagtttgagg atcgccagcc cccctgccaa catgatgata atggcgatca
    39541 cccacgcaaa aatcgggcga tcgataaaga aattaggcat gtcttaacgg ctcctgttta
    39601 agttaagact tggactgttc aggctgagca ccgcttgcgg cttgctggtt attatcagcg
    39661 gtaacttctt gtgcttttac ctggacacca ggacgcactt tctgcagccc acttattact
    39721 acgcgatcgc ctgctttcag accttctgtc accagccact tatcgccaat agcctggctt
    39781 gcaacgatcg gacgggtttc cactttgtca tccgcgccaa ctaccagtac ggtggcatca
    39841 ccacgcggcg tacgggttac gccctgttgc gggactaaaa tagcgtttgg attaagccct
    39901 tcttccagac gtgcgcgcac gaacataccc ggcagcagag tgtgatccgg gttcgggaag
    39961 atagcgcgta gggtgataga cccagtggtc tgatcaacgg taacgtcaga gaattccagc
    40021 gtaccgtcct gcgggaactt aatgccgtca ctggtgatca gcgacacttt ggctttgccg
    40081 ttctcttgtt tcagcgtgcc attcgccagt tcctgtttca ggcgcaggaa gtcgttgctg
    40141 gactgggtca catcaacgta gatcggatca agttgctgca cggttgccag cgcagtcgcc
    40201 tgaccgttct gtaccaatgc gccttccgtc acgttcgact taccaatgcg accgctaatc
    40261 ggagaggtga ctttggtgta agccagattg atccgcgcag tttcaacggc agctttcgcc
    40321 gcagttaccg cagcattcgc ctgttgcgca tcagccagag cctgatcgta ctcttgctta
    40381 ctgatgtact gagtaccgag cagtttctga taacgattca ccgtcaattg cgcgatattg
    40441 gctgcagcct gggctttcgc cagatcacct ttcgcactgt cgtatgtcgc ctgataggtc
    40501 gcaggatcaa tctgatagag agagacacca gcttcgatgt cgctaccttc tttgaaatta
    40561 cgcttcagga taatcccgct aacttgagga cgaacttctg cgatccggta ggcactggtg
    40621 cgacccggaa gctcggttgt gatctgcaga ggttcagttt tgactgttac tacgccaacg
    40681 gcgggcatct gctggccacc ttgttgggcc tgtttgtcgt cacatcctgt tagggctaag
    40741 ctgcctgaga gcatcagaac gaccgccaga ggcgtaaacc ctctgttttt gttcatatgt
    40801 aaacctcgag tgtccgattt caaattggtc aatggtcaaa agttaataaa cccattgctg
    40861 cgtttatatt atcgtcgtgc tatggtacat acattcacaa atgtatgtaa atctaacgcc
    40921 tgtaaattca cgaacatatg gcacgaaaaa ccaaacaaga agcgcaagaa acgcgccaac
    40981 acatcctcga tgtggctcta cgtcttttct cacagcaggg ggtatcatcc acctcgctgg
    41041 gtgagattgc aaaagcagct ggcgttacgc gcggtgcaat ctactggcat tttaaagaca
    41101 agtcggattt gttcagtgag atctgggaac tgtcagaatc caatattggt gaactagagc
    41161 ttgagtatca ggcaaaattc cctggcgatc cactctcagt attaagagag atattaattc
    41221 atgttcttga atccacggtg acagaagaac ggcgtcgatt attgatggag attatattcc
    41281 acaaatgcga atttgtcgga gaaatggctg ttgtgcaaca ggcacaacgt aatctctgtc
    41341 tggaaagtta tgaccgtata gaacaaacgt taaaacattg tattgaagcg aaaatgttgc
    41401 ctgcggattt aatgacgcgt cgcgcagcaa ttattatgcg cggctatatt tccggcctga
    41461 tggaaaactg gctctttgcc ccgcaatctt ttgatcttaa aaaagaagcc cgcgattacg
    41521 ttgccatctt actggagatg tatctcctgt gccccacgct tcgtaatcct gccactaacg
    41581 aataaccctg aatctgactc caggattttt cctggacatt ttcgtcgttg ctattctggt
    41641 tcactgcgtc gtgatattct tgcggtttga ctttttcagg tcgttcttca ggttcagaaa
    41701 ccttcattca tcatgactat gttccagtat tacaaacgat cacggcattt tgttttttca
    41761 gcatttattg cttttgtttt tgtcttgtta tgccagaaca cggcgtttgc gcgggcgtca
    41821 tcgaatggtg atctgccgac aaaagcggac ctgcaggcgc aacttgactc actaaataaa
    41881 caaaaagatc tttctgctca ggacaaactg gtgcagcagg atctgacaga tacattagcc
    41941 accctcgata aaatcgatcg cataaaagaa gagacagttc agctacggca aaaagtcgct
    42001 gaagcgccgg aaaaaatgcg ccaggcgacc gcggcgttaa cagcacttag cgatgtcgat
    42061 aacgacgaag aaacgcgcaa aattctgagt acgctttcgt tgcgccagct ggaaactcgc
    42121 gttgcccagg cgctggacga tttgcaaaac gcacaaaacg atctggcgtc ttataacagc
    42181 cagctggttt cgttacagac gcagcccgaa cgcgtgcaaa atgcgatgtg taacgcttcg
    42241 cagcagctgc aacaaattcg cagtcgtctg gatgggactg atgtcggcga gactgcctta
    42301 cgtcccagcc agaaagtgtt aatgcaggct cagcaggcgt tgctgaatgc ggagattgac
    42361 cagcagcgta aaagcctgga agggaacacc gtcttgcagg ataccttgca aaagcaacgt
    42421 gattacgtga cggcgaacag cgcccgtctg gagcaccagt tgcagctgtt gcaagaagcg
    42481 gtaaacagca agcgcctgac tttaactgaa aaaacggcgc aggaagccgt ctccccggat
    42541 gaagccgcgc gtattcaggc taatccgctg gtgaaacagg aactggaaat taaccagcag
    42601 ttaagtcagc gtctgattac cgcgactgaa aacggtaatc agttgatgca gcaaaacatt
    42661 aaagtcaaaa actggctgga gcgggcgctg caatcggaac gcaatattaa agagcagatt
    42721 gccgtcctga agggcagcct gctgttgtct cgtatccttt accagcaaca acaaacgctg
    42781 ccctcggcgg atgaactgga aaacatgacc aaccgcatcg cggatttgcg tctcgaacag
    42841 tttgaagtta accagcagcg tgatgcactc ttccagagcg atgcgttcgt caacaaactg
    42901 gaagaaggtc acaccaacga agtcaacagc gaagttcacg atgcgttatt gcaagtggtt
    42961 gatatgcgtc gcgaattgct ggatcaactc aacaaacagt tgggtaacca gctgatgatg
    43021 gccattaacc tgcaaatcaa ccagcagcag ttaatgagcg tatcgaaaaa cctgaaatcc
    43081 atcctgactc agcaaatctt ttgggtgaac agtaaccgtc caatggactg ggactggatc
    43141 aaagcgttcc cgcaaagcct gaaagatgaa tttaagtcga tgaaaatcac ggtgaactgg
    43201 gaaaaagcct ggcctgccgt ttttatcgct ttcctcgccg gtttgccgct gctgttgatt
    43261 gccgggctga tccactggcg tctgggctgg ctgaaagcgt atcaacagaa actggcttcc
    43321 gctgtgggtt ccctgcgtaa cgatagccag ctcaacacac caaaagcgat ccttatcgac
    43381 ctgatccgcg cgctgccggt gtgcctgatt attctcgcgg ttggcctgat tctgttgacc
    43441 atgcagctca acatcagcga actgctatgg tcgttcagta aaaaactggc gattttctgg
    43501 ctggtgtttg gcctgtgctg gaaggtactg gagaaaaatg gcgttgccgt gcgtcacttc
    43561 ggcatgccgg agcagcagac cagccactgg cgtcggcaaa ttgtccgcat cagtctcgcg
    43621 ttgctgccta tccatttctg gtctgtggtg gcagaacttt ccccgctgca tctgatggat
    43681 gatgtgctgg ggcaggcgat gattttcttc aacctgctgc tgattgcctt cctggtatgg
    43741 ccaatgtgcc gcgaaagctg gcgtgataaa gagtcgcaca ccatgcgact ggtcaccatt
    43801 accgtgctgt cgattatccc gattgcattg atggtgctga ctgccacggg ttatttctac
    43861 accacgctgc gcctctccgg gcgttggatt gaaaccgttt atctggtgat catctggaac
    43921 ttgctctacc agacggtatt gcgcggctta agcgtagcgg cgcggcgtat cgcctggcgt
    43981 cgtgcgctgg cgcgtcggca gaatctggtg aaagagggcg cagaaggtgc cgaaccgccg
    44041 gaagaaccca ccattgcact ggaacaggtt aaccagcaga cgctgcgtat taccatgttg
    44101 ctgatgtttg cgctgttcgg tgtcatgttc tgggcaattt ggtccgattt gatcaccgtg
    44161 ttcagctatc tcgacagcat cacgctctgg cattacaacg gcactgaagc tggcgctgcg
    44221 gtggtgaaaa acgtcaccat gggcagtctg ttgtttgcga ttatcgcctc aatggtggcc
    44281 tgggcgttga ttcgcaacct gcctggttta ctggaagtgc tggtgctctc gcgactgaat
    44341 atgcgtcagg gcgcgtcgta tgccattact accatcctta actacatcat tattgccgtt
    44401 ggtgcgatga cggtgttcgg atcgctgggc gtctcttggg ataaactcca gtggctggcc
    44461 gcagcattat ccgtaggtct tggttttggt ttacaagaaa ttttcggtaa cttcgtctcc
    44521 ggtttgatca ttctgttcga acgtccggtg cgtattggcg atacggtaac cattggtagc
    44581 ttctcgggga cggtaagtaa gatccgtatt cgtgcgacaa cgattaccga tttcgatcgc
    44641 aaagaagtga tcatcccgaa caaagcgttt gttaccgagc gtctgatcaa ctggtcgttg
    44701 actgacacta ctacgcgtct ggtgatccgt ctcggcgtgg cctatggctc cgatctggaa
    44761 aaagtgcgta aagtgttact gaaggcggcg actgagcacc caagggtgat gcacgaacca
    44821 atgccggaag tcttctttac ggcgtttggt gccagcacgt tggatcatga gctgcgtctg
    44881 tatgtgcgtg aactgcgtga ccgtagtcgt actgtcgatg agctgaaccg tactatcgat
    44941 cagctgtgcc gtgaaaacga catcaacatt gcctttaacc agcttgaagt gcatctgcac
    45001 aacgagaagg gcgatgaggt gacggaagtc aaacgcgact acaaaggcga tgacccgacg
    45061 ccagcggtag ggtaataacc aaagggggcg acatttagtt gccccgagat tactgacaaa
    45121 gtgcgcgttg ttcatgccgg atgcggcgtg accgccttat ccggcctacg aaaccgcaag
    45181 aattcaatgt attgcaggag cggtgtagat actgttatgg cctgcaagtt ttttcctggt
    45241 ccggtttccc ggattgcagg catgccagac acagcgccgt caggcgctgt ttttttcatc
    45301 ccttccgcga tttttacgcc gctaccggat tatgccgtga cgcatcgaac ggcacgcctg
    45361 acttcagcac tccatacgcc acctgtgcca gcttgcgcat catcgcgccg agaatcacct
    45421 ttcctttctt gccatta
//
LOCUS       DAZGTR010000023        44664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGTR010000023 DAZGTR010000000
VERSION     DAZGTR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.023"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..96
                     /locus_tag="ACHLCY_001468"
     CDS             <1..96
                     /locus_tag="ACHLCY_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFP0368762.1"
                     /translation="TLLLTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            120..4634
                     /locus_tag="ACHLCY_001469"
     CDS             120..4634
                     /locus_tag="ACHLCY_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP0368763.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTMALSKVSGNELLVWTAGKKQGEAKPGSESLWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     DARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSFADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQSLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNISVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            4635..6284
                     /gene="yfaQ"
                     /locus_tag="ACHLCY_001470"
     CDS             4635..6284
                     /gene="yfaQ"
                     /locus_tag="ACHLCY_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP0368764.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            6289..7065
                     /gene="yfaP"
                     /locus_tag="ACHLCY_001471"
     CDS             6289..7065
                     /gene="yfaP"
                     /locus_tag="ACHLCY_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP0368765.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            7340..10189
                     /gene="rcsC"
                     /locus_tag="ACHLCY_001472"
     CDS             7340..10189
                     /gene="rcsC"
                     /locus_tag="ACHLCY_001472"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP0368766.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(10389..11039)
                     /gene="rcsB"
                     /locus_tag="ACHLCY_001473"
     CDS             complement(10389..11039)
                     /gene="rcsB"
                     /locus_tag="ACHLCY_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP0368767.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(11056..13728)
                     /gene="rcsD"
                     /locus_tag="ACHLCY_001474"
     CDS             complement(11056..13728)
                     /gene="rcsD"
                     /locus_tag="ACHLCY_001474"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP0368768.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETSLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQSRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            14467..15558
                     /gene="ompC"
                     /locus_tag="ACHLCY_001475"
     CDS             14467..15558
                     /gene="ompC"
                     /locus_tag="ACHLCY_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0368769.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            15670..16725
                     /gene="apbE"
                     /locus_tag="ACHLCY_001476"
     CDS             15670..16725
                     /gene="apbE"
                     /locus_tag="ACHLCY_001476"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP0368770.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            16799..17863
                     /gene="ada"
                     /locus_tag="ACHLCY_001477"
     CDS             16799..17863
                     /gene="ada"
                     /locus_tag="ACHLCY_001477"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP0368771.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANACEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            17863..18513
                     /gene="alkB"
                     /locus_tag="ACHLCY_001478"
     CDS             17863..18513
                     /gene="alkB"
                     /locus_tag="ACHLCY_001478"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP0368772.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            18589..20232
                     /gene="yojI"
                     /locus_tag="ACHLCY_001479"
     CDS             18589..20232
                     /gene="yojI"
                     /locus_tag="ACHLCY_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP0368773.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            20450..22096
                     /gene="mqo"
                     /locus_tag="ACHLCY_001480"
     CDS             20450..22096
                     /gene="mqo"
                     /locus_tag="ACHLCY_001480"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP0368774.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQNSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(22245..22754)
                     /gene="eco"
                     /locus_tag="ACHLCY_001481"
     CDS             complement(22245..22754)
                     /gene="eco"
                     /locus_tag="ACHLCY_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP0368775.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(22869..23033)
                     /gene="yojO"
                     /locus_tag="ACHLCY_001482"
     CDS             complement(22869..23033)
                     /gene="yojO"
                     /locus_tag="ACHLCY_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP0368776.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            23142..23636
                     /gene="napF"
                     /locus_tag="ACHLCY_001483"
     CDS             23142..23636
                     /gene="napF"
                     /locus_tag="ACHLCY_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP0368777.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            23626..23889
                     /gene="napD"
                     /locus_tag="ACHLCY_001484"
     CDS             23626..23889
                     /gene="napD"
                     /locus_tag="ACHLCY_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP0368778.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            23886..26372
                     /gene="napA"
                     /locus_tag="ACHLCY_001485"
     CDS             23886..26372
                     /gene="napA"
                     /locus_tag="ACHLCY_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFP0368779.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            26379..27074
                     /gene="napG"
                     /locus_tag="ACHLCY_001486"
     CDS             26379..27074
                     /gene="napG"
                     /locus_tag="ACHLCY_001486"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP0368780.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            27061..27924
                     /gene="napH"
                     /locus_tag="ACHLCY_001487"
     CDS             27061..27924
                     /gene="napH"
                     /locus_tag="ACHLCY_001487"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP0368781.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            27921..28370
                     /gene="napB"
                     /locus_tag="ACHLCY_001488"
     CDS             27921..28370
                     /gene="napB"
                     /locus_tag="ACHLCY_001488"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP0368782.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGALRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            28380..28982
                     /gene="napC"
                     /locus_tag="ACHLCY_001489"
     CDS             28380..28982
                     /gene="napC"
                     /locus_tag="ACHLCY_001489"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP0368783.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            28995..29618
                     /gene="ccmA"
                     /locus_tag="ACHLCY_001490"
     CDS             28995..29618
                     /gene="ccmA"
                     /locus_tag="ACHLCY_001490"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP0368784.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            29615..30277
                     /gene="ccmB"
                     /locus_tag="ACHLCY_001491"
     CDS             29615..30277
                     /gene="ccmB"
                     /locus_tag="ACHLCY_001491"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP0368785.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VSGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGAL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            30319..31056
                     /gene="ccmC"
                     /locus_tag="ACHLCY_001492"
     CDS             30319..31056
                     /gene="ccmC"
                     /locus_tag="ACHLCY_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP0368786.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            31053..31262
                     /gene="ccmD"
                     /locus_tag="ACHLCY_001493"
     CDS             31053..31262
                     /gene="ccmD"
                     /locus_tag="ACHLCY_001493"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP0368787.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            31259..31738
                     /gene="ccmE"
                     /locus_tag="ACHLCY_001494"
     CDS             31259..31738
                     /gene="ccmE"
                     /locus_tag="ACHLCY_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP0368788.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            31735..33678
                     /gene="ccmF"
                     /locus_tag="ACHLCY_001495"
     CDS             31735..33678
                     /gene="ccmF"
                     /locus_tag="ACHLCY_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP0368789.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            33675..34232
                     /gene="dsbE"
                     /locus_tag="ACHLCY_001496"
     CDS             33675..34232
                     /gene="dsbE"
                     /locus_tag="ACHLCY_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP0368790.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            34229..35281
                     /gene="ccmH"
                     /locus_tag="ACHLCY_001497"
     CDS             34229..35281
                     /gene="ccmH"
                     /locus_tag="ACHLCY_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP0368791.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(35316..35963)
                     /gene="narP"
                     /locus_tag="ACHLCY_001498"
     CDS             complement(35316..35963)
                     /gene="narP"
                     /locus_tag="ACHLCY_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP0368792.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            36283..38873
                     /gene="yejO"
                     /locus_tag="ACHLCY_001499"
                     /pseudo
     CDS             36283..38873
                     /gene="yejO"
                     /locus_tag="ACHLCY_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(39340..40263)
                     /locus_tag="ACHLCY_001500"
     CDS             complement(39340..40263)
                     /locus_tag="ACHLCY_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0368793.1"
                     /translation="MISKNMKGVSAVRAASVLTSCKFSHADAIEMTKKNDPAVSKFLE
                     KSIVAGMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLQSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPCTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIELFKKHIANGNTLATSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGTV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            complement(40253..40414)
                     /locus_tag="ACHLCY_001501"
     CDS             complement(40253..40414)
                     /locus_tag="ACHLCY_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368794.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQPTEIRLQAAAVLMPYFHETLTPC
                     YQEEEDDDF"
     gene            complement(40426..40914)
                     /locus_tag="ACHLCY_001502"
     CDS             complement(40426..40914)
                     /locus_tag="ACHLCY_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0368795.1"
                     /translation="MALTTKKKRFADALSKGTNPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDQKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAANVSSNRFPIPKPPTGL
                     KH"
     gene            complement(41042..41203)
                     /locus_tag="ACHLCY_001503"
     CDS             complement(41042..41203)
                     /locus_tag="ACHLCY_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFP0368796.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSDEVE
                     GLISLLGEQ"
     gene            complement(41207..41401)
                     /locus_tag="ACHLCY_001504"
     CDS             complement(41207..41401)
                     /locus_tag="ACHLCY_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368797.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDIVRRMNESGIYGRAATPT
                     IGHLARVLLRERLGIATSEA"
     gene            complement(41532..41777)
                     /locus_tag="ACHLCY_001505"
     CDS             complement(41532..41777)
                     /locus_tag="ACHLCY_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368798.1"
                     /translation="MNRKTRDKTAPKYKALDMTELSLKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSAGE"
     gene            complement(41979..43379)
                     /locus_tag="ACHLCY_001506"
     CDS             complement(41979..43379)
                     /locus_tag="ACHLCY_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP0368799.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSQKRENWREYLERERGKKPGEEKHHQRKTTLPMSVGSAGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHGEQVKNLYLVNRPIFPAAPLDVEEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRPPV
                     ELERIEEAGNKTENHAALWQCIRTRTAHGEACTIALLRDDMKKLGYEMKHFRRWLYKL
                     ENDGVIVIDGDDVRPL"
     gene            complement(43376..43675)
                     /locus_tag="ACHLCY_001507"
     CDS             complement(43376..43675)
                     /locus_tag="ACHLCY_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0368800.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVAGRCVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            complement(43681..43914)
                     /locus_tag="ACHLCY_001508"
     CDS             complement(43681..43914)
                     /locus_tag="ACHLCY_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368801.1"
                     /translation="MRDIYLETIDRASLALSHSENMMEILRMCLESFGDNERNAKKTR
                     IITSLITLLESVINELQEIETLHDRYNEQHTGE"
     gene            complement(43907..44353)
                     /locus_tag="ACHLCY_001509"
     CDS             complement(43907..44353)
                     /locus_tag="ACHLCY_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368802.1"
                     /translation="MCNKTTPDAAAALTTLIHALIDIECTAELAQGEEQKDRTQFALE
                     CIRYIATRSLNDAKNILVADCENGGGVMRDDRFNSLKQEFSGVPDDAADALSSMPELI
                     RAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            complement(44569..>44664)
                     /locus_tag="ACHLCY_001510"
     CDS             complement(44569..>44664)
                     /locus_tag="ACHLCY_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0368803.1"
                     /translation="IEATSEQEARLQSPAGCVMVFAARIRQGVGL"
BASE COUNT        10349 a        11073 c        12061 g        11181 t
ORIGIN      
        1 actctccttc tgacatggaa ggacacccga tggataccca acgattccaa tcccaatttc
       61 attggcattt atcgtttaaa ttttctggcg cgatagcagc gtgcttgtcc ctgtctcttg
      121 tgggaacagg gcttgctaat gctgatgatt cgcttccttc cagtaactat gcgccgcccg
      181 ccgggggaac attctttttg cttgctgaca gcagttttag cagcagtgaa gaggcgaaag
      241 tgcgactgga agcgccgggg cgtgattatc ggcgctatca gatggaagag tacggcggcg
      301 tggacgttcg cctgtatcgt attcctgacc cgatggcatt tttgcgccag cagaaaaacc
      361 tgcatcgcat tgtggtgcaa ccgcaatatc tgggcgacgg gctgaacaat acgctgacct
      421 ggctgtggga taactggtac ggcaaatctc gccgcgtaat gcagcgtact ttctcttctc
      481 agtcacggca gaatgtgact caggcattac ccgaattaca tctcggcaat gccattatta
      541 aaccttcccg ttatgtacag aacaaccagt tttccccgct gaaaaaatat cccttggtgg
      601 aacagttccg ttatccacta tggcaggcta aaccggtcga gccgcagcaa ggggtaaaac
      661 tggaaggcgc atccagcaat ttcatctcgc cgcagccggg taacatttat attcctctcg
      721 gccaacaaga gccgggactg tacctcgtcg aggcgatggt tggtgggtat cgggcgacga
      781 cggtggtgtt tgtttccgat accatggcgc ttagcaaagt gtcaggcaac gagcttctgg
      841 tgtggaccgc gggtaaaaaa cagggtgaag cgaagcccgg ctcagagagc ttgtggactg
      901 acggtcttgg cgtgatgacc cgcggtgtga ccgatgacag cggtaccttg cagttacaac
      961 atatatcgcc agaacgttca tacattctgg gtaaggatgc tgaaggcggt gtttttgtct
     1021 ccgagaactt cttctacgaa agcgaaatct acaacacccg cttgtatatt tttaccgatc
     1081 gcccgctata tcgcgcaggc gatcgtgtcg atgttaaagt gatgggccgc gagttccacg
     1141 atccgttgca ttcatccccc atcgtcagcg ccccggcgaa gctttcggtg ctggacgcta
     1201 acggcagtct gttgcaaacc gtcaatgtca cgctggatgc gcgcaatggc gggcagggaa
     1261 gtttccgcct gccagaaaat gccgtagccg gaggttatga gttacgtctt gcttaccgca
     1321 atcaggtcta tagcagcagt tttcgcgtgg caaactacat caagccacat ttcgagattg
     1381 gtttagctct cgacaaaaaa gagttcaaaa ctggcgaagc ggtcagcggc aaactgcaac
     1441 tgctttaccc ggatggtgag ccggtaaaag atgcccgcgt gcagttaagt ttgcgcgctc
     1501 agcaattatc aatggtcggt aacgatttgc gttatgccgg acgttttccc gtgtcgctgg
     1561 aaggcagcga aacggtgtcc gacgccagcg gtcatgtggc gttaaatctc cccgccgccg
     1621 ataaaccgag ccgctatttg ttaaccgtct ccgccagtga cggcgcggcg tatcgcgtca
     1681 ccaccaccaa agagatcctc attgaacgcg gcctggcgca ttactcttta agtaccgccg
     1741 cacaatacag taatagcggc gagtcggttg tgttccgtta tgccgcgctg gaatcttcaa
     1801 aacaggttcc tgttacgtat gaatggttgc gtctcgaaga ccgcacgagc catagcggag
     1861 agctaccgtc aggcggcaaa tcctttaccg tcaatttcgc taaacctggc aactacaatc
     1921 tgacattacg cgataaagac ggcttaattc tcgctgggtt aagtcatgcc gtcagcggta
     1981 agggcagcac ggcgcatact ggtacggtag atatcgtggc ggataaaacg ctgtaccagc
     2041 caggcgaaac tgcgaagatg ctgattacct ttccggagcc aattgatgaa gcattattga
     2101 cgctggaacg cgatcgcgtg gaacagcagt cgctgctttc gcatccggca aactggctga
     2161 cgttacaacg tttaaacgat acccagtatg aagcccgggt tccagtgagc aattcctttg
     2221 cgcctaacat cactttttcg gtgctgtata cccgtaatgg tcagtacagt tttcagaacg
     2281 ccgggatcaa agttgccgtt cctcagcttg atatccgggt gaaaacggac aaaacccatt
     2341 accagcctgg tgaactggtc aatgtcgaat taacctcgtc gctgaaaggt aaacctgttt
     2401 ctgcgcagct aacggtaggc gtggtcgatg aaatgatcta cgcgctgcaa ccagaaatcg
     2461 cgccgaatat cggcaaattt ttctatccgc tggggcgtaa caatgtgcgt accagctcca
     2521 gtctgtcgtt tatcagctac gaccaggcgc tctccagcga gccggttgcg cctggcgcaa
     2581 ctaaccgcag cgagcggcga gtaaaaatgc ttgaacgtcc acggcgtgaa gaggtggata
     2641 ccgcggcatg gatgccgtca ctcacaaccg ataaacaagg caaagcgtat ttcacgttcc
     2701 tgatgcctga ttcgttaacc cgctggcgta tcaccgcgcg tgggatgaac ggcgacgggc
     2761 tggtcgggca ggggcgtgct tatctgcgtt cggaaaaaaa tctctacatg aagtggagta
     2821 tgccaacggt gtatcgcgtg ggcgacaaac cggcggcagg actgtttatc ttcagtcagc
     2881 aggataacga accggtggcg ctggtgacta aatttgcagg cgctgagatg cgccagacgc
     2941 tgacgctgca caaaggggcg aattatattt cgctgacgca gaacattcag caatctggct
     3001 tgttaagtgc agaactgcaa caaaatgggc aagtgcagga cagcattagc acaaaactgt
     3061 cttttgtgga caacagctgg cccgttgaac agcagaaaaa tgtcatgctc ggcggtggcg
     3121 agaacgcgct gatgttgccc gagcaggcga gtaatatccg gctacaaagt agtgaaacgc
     3181 cgcaggagat tttccgcaac aatcttgatg cgttagtcga tggaccatgg ggtggcgtga
     3241 tcaacaccgg tagccgtctg atcccgctca gtctcgcctg gcgttcgttt gccgatcatc
     3301 aaagtgccgc cgctaacgac attcgtcaga tgattcagga taaccgtctg cggctgatgc
     3361 aactggcggg gcccggagcg cgctttacct ggtggggtga agatggcaat ggtgacgcct
     3421 tccttacggc atgggcatgg tacgccgact ggcaggccag ccaggcgctc ggcgtaacgc
     3481 aacaaccgga atactggcag catatgctcg acagttatgc cgagcaggcg gataacatgc
     3541 cgttattgca tcgggcgctg gtgctggcgt gggcacagga gatgaatctg ccgtgcaaaa
     3601 cgttgttgaa agggttggat gaagctatcg cccggcgcgg aactaaagat gaagatttct
     3661 ctgaggaaga catccgcgat atcaatgaca gcctgatcct cgatacaccg gaatctccac
     3721 tggcagatgc ggtggcaaac gtcttaacca tgacgttgct gaaaaaagcg cagttgaagt
     3781 ccacggtgat gccacaggtt cagcaatatg cgtgggataa agcggcaaac agcaatcagt
     3841 cgctggcgca cacggttgtg ctgctcaata gcggtggtga cgctacccag acggccgcta
     3901 ttttaagtgg tttgaccgct gagcaatcca ctattgagcg cgcgctggcc atgaactggc
     3961 tggcgaaata tatggcgaca atgcctccag ttgttttgcc tgcgcctgcg ggcgcatggg
     4021 ctaaacataa gttaactgga gggggcgaag actggcgttg ggttggtcag ggcgtgccgg
     4081 acattctctc ttttggtgac gaattatcgc cgcaaaatgt gcaggtccgc tggcgtgagc
     4141 cggcaaaaat ggctcaacaa agtaacattt cggtgaccgt tgaacgccag ttgtatcggc
     4201 ttatccctgg tgaagaagag atgagcttta ttctgcaacc ggtgaccagc aatgagattg
     4261 acagcgatgc gctgtatctc gatgaaatta cgcttaccag cgagcaggat gcagttctgc
     4321 gctatggtca ggtggaagta ccgctcccgc cgggagccga cgttgagcgc acaacatggg
     4381 gcatttcagt caataaaccc aacgccgcga aacagcaggg gcaattgctg gaaaaagcgc
     4441 gtaatgaaat gggcgaactg gcctatatgg tgccggtgaa agaactgacg ggaacggtca
     4501 ctttccgcca tttgctgcgc ttctcgcaaa aagggcaatt cgttctgcct cctgctcgtt
     4561 atgtgcgttc ctatgcacct gcgcagcaaa gtgttgcggc agggagtgaa tggaccggga
     4621 tgcaggtgaa ataagtgaac tggcgcagaa tcgtctggct attggcgttg gttactctgc
     4681 caacgcttgc ggaggagacg ccgttacaac tggtgctgcg cggtgcgcag cacgatcaac
     4741 tgtatcagct gtcgtcatca ggcgtgacca aagtctcagc attacctgac tcgctcacca
     4801 cgccgttagg gagtttgtgg aaactgtatg tttacgcctg gctggaggat acccatcaac
     4861 cggagcagcc ctaccagtgt cgcggaaact ccccggaaga ggtttattgt tgtcaggctg
     4921 gggagagcat aacgcgcgat accgcgctgg tacgttcctg tgggctctat tttgcgccgc
     4981 agcggttgca tattggcgca gatgtgtggg ggcaatactg gcaacagcgt caggcacctg
     5041 cgtggttagc gtctcttaca acgctgaagc cagaaacctc ggtaacggtt aaatcgctgc
     5101 tcgattcatt agcgacgtta cctgcgcaaa acaaagctca ggaagtctta ctggatgtgg
     5161 tgctggacga ggcgaaaatc ggtgtcgcat ccatgctcgg tagtcgggta cgggtgaaaa
     5221 cctggagctg gtttgccgat gataaacagg aaatccgcca gggtggtttt gctggctggt
     5281 taaccgatgg tacaccgctg tgggttactg gcagtggcac cagcaaaacg gtactaaccc
     5341 gttatgcgac ggtgcttaat cgggttttgc cagtgccgac gcaggttgcc agtgggcaat
     5401 gtgtagaggt ggagctgttt gcccgctatc cgctgaagaa aatcacagcg gaaaagagta
     5461 cgacggcagt aaaacccggc gtacttaatg ggcgctatcg tgtgacgttt actaatggta
     5521 accacattac gtttgtcagc cacggtgaga cgaccttact gagcgaaaag ggcaaactga
     5581 aattgcagtc gcatctcgat cgtgaagagt acgttgccag agttctggat cgcgaggcga
     5641 aaagtacgcc ccctgaggca gcgaaagcca tgaccgtggc gattcgcact tttttacagc
     5701 aaaacgccaa tcgggaaggc gattgcctga ccatccccga cagcagcgcc acgcagcgtg
     5761 tttctgcttc gccagcgacc acgggggcgc gaacgatgac cgcctggacg caggatctca
     5821 tctatgcagg cgatccggtt cattatcatg gcagtcgagc taccgaaggg acactttcct
     5881 ggcggcaagc cacggcccag gcggggcagg gagagcgtta cgaccagata ctcgcgtttg
     5941 cttatcccga caacagcctt agccgctggg gagcgccacg ctcaacctgc cagttattac
     6001 ccaaagcgaa agcctggctg gcgaaaaaaa tgccgcagtg gcggcgtata ttacaaggcg
     6061 agacggggta caacgaacca gacgtgtttg cggtctgtcg tttagtctca ggtttcccct
     6121 ataccgatcg gcagcagaaa cggctgttta ttcgtaactt ctttacgctt caggatcggc
     6181 tggatttaac ccatgagtat ctgcacctgg cctttgatgg ttatcccacc gggcttgatg
     6241 agaactatat cgaaacgctg acccgacaat tattaatgga ctaatgctat gcgaaagatt
     6301 tttcttccgt tgttactggt ggcactttcg cctgtcgctc acagtgaagg tgtgcaggaa
     6361 gtcgagattg acgcaccgct ctctggctgg catccggcag agggcgaaga tgccagtttt
     6421 tcacaatcga tcaattaccc ggcgtcgtcc gtcaacatgg cagatgatca aaatatctca
     6481 gcgcagatcc gcggcaaaat aaagaattat gctgcggcgg gtaaagttca gcagggtcgg
     6541 ctggtggtca acggtgccag tatgccgcag agaattgaat ccgatggttc atttgcacgc
     6601 ccttatattt tcactgaagg cagcaacagc gtgcaggtca tcagcccgga tgggcaaagc
     6661 cggcaaaaaa tgcagtttta ctcaacgccg ggtacaggaa cgattcgtgc acgtttacgg
     6721 ctggttctct cgtgggatac ggacaatacc gatctcgatc tccatgttgt cacgcccgat
     6781 ggcgaacacg cctggtacgg taacaccgtg ctgaaaaaca gtggtgcact ggatatggat
     6841 gtcacgacgg ggtacggacc cgagattttc gccatgccag caccagttca cgggcgctat
     6901 caggtgtata tcaactacta tggtgggcgc agcgaaacgg aattgacgac cgcccaactg
     6961 acgctgatca ccgatgaagg atcggtcaat gagaaacagg aaacatttat tgtaccgatg
     7021 cgtaatgctg gcgagctgac gctggtgaaa agttttgact ggtgatccat cccttcacac
     7081 aacctggctc tctctttttt cagataagac tgctgggcga tctgccgacg ccaggatata
     7141 accattaaca agcgtcggca gattggtcat ttcttatttt tcgtactgag aactgtccat
     7201 ctactccggc tttcctggtt aaggcaatgg tttctaagcg atacgcatta ttgcaatggt
     7261 gacagaacat tgcggtaaca cgcttttacc gctaccttaa ccacactcca tcggtcacct
     7321 gaggcggagc ttcgcccctt tgaaatacct tgcttctttt cgtacaaccc tgaaagcctc
     7381 gcgctacatg ttcagagcat tggcgttagt gctctggctg ttgattgctt tttcatccgt
     7441 tttttacatc gttaatgcgt tacatcagcg agaatcggaa attcgtcagg aatttaatct
     7501 gagttccgat caggctcagc gctttattca acgcacctct gatgtgatga aagagctgaa
     7561 gtacatcgcc gaaaatcgct tatcggcaga aaacggtgtg ctttccccgc gtggacgaga
     7621 aacgcaggcg gatgtgcctg cgtttgaacc gctgtttgcc gactccgatt gttccgcaat
     7681 gagtaacacc tggcgaggtt ctctggagtc attggcgtgg tttatgcgct actggcgcga
     7741 taatttttct gcggcttacg atctcaaccg ggtattttta atcggcagtg ataacctctg
     7801 catggccaat ttcggtctgc gtgatatgcc agtggaacgc gataccgcgt tgaaagcgtt
     7861 gcatgaacgc atcaataaat atcgaaatgc accacaagat gatagcggca gtaacctcta
     7921 ctggatcagc gaaggtccgc gccctggcgt cgggtatttt tacgcgttga cgccagttta
     7981 tctggcgaac cggttgcagg cgcttttggg tgtcgagcag accatccgga tggaaaactt
     8041 tttcttaccg ggtacgttgc cgatgggggt taccattctt gatgaaaatg gtcataccct
     8101 gatttcgctt accggaccag aaagtaaaat taagggcgat cctcgctgga tgcaggaacg
     8161 ctcctggttt ggctatacgg aagggttccg ggagctggtg ctgaagaaaa atctgccacc
     8221 ctcatcgcta agcatcgtgt attcggtgcc ggttgataag gtgctggaac gcattcgcat
     8281 gttgatcctt aacgcaattt tgctgaatgt gcttgccgga gctgcattgt ttactctcgc
     8341 acggatgtac gagcgacgta ttttcattcc ggcggaaagc gacgccctgc gactggaaga
     8401 acatgagcag ttcaatcgca agattgtcgc ctccgcgcca gtgggtatct gcattttgcg
     8461 taccgctgat ggcgtcaata ttttaagtaa cgaactggcg catacctatc tcaatatgct
     8521 tacgcatgag gaccgccaac gactgacgca aattatctgt gggcagcagg tcaattttgt
     8581 tgatgtcctg accagcaaca ataccaatct gcaaatcagc ttcgtccatt cgcgctatcg
     8641 taatgaaaac gtggccattt gtgtgctggt ggatgtttct tcgcgcgtga agatggaaga
     8701 gtcgttgcag gagatggcac aagcagcgga acaggcgagc cagtcaaaat cgatgttcct
     8761 tgccaccgtc agtcatgagc tgcgaacgcc gctgtatggc attatcggta acctggatct
     8821 gttgcaaacc aaagagttac cgaaaggcgt cgatcggctg gtgacggcaa tgaacaactc
     8881 ttccagcctg ttgttgaaaa ttatcagcga tattctcgat ttctcgaaga ttgaatcgga
     8941 acagttgaag atcgaaccgc gtgagttttc accgcgtgaa gtgatgaacc acatcaccgc
     9001 caactattta ccgctggtgg tacgcaagca gttaggcttg tactgcttta ttgaaccgga
     9061 tgtgccagtg gccttaaatg gcgacccgat gcgtttacag caggtcatct ccaacctgct
     9121 gagtaacgcc ataaaattca ccgataccgg ctgtatagtt ttgcatgttc gcgctgatgg
     9181 cgattatctc tctatccgtg ttcgcgatac cggcgtgggc attccggcga aagaagtggt
     9241 gcgcttgttt gatcccttct tccaggtcgg aacgggcgta cagcgtaact tccaggggac
     9301 cggtctgggt ctggcgattt gtgaaaaact gatcagcatg atggacggcg atatctcggt
     9361 agattcagaa ccgggaatgg gcagccagtt taccgtgcgt attccgttgt acggcgctca
     9421 gtacccgcag aaaaaaggcg tggaagggtt gagtggtaaa cgctgctggc tggcggtccg
     9481 caatgcgtcg ctctgtcagt tcctggaaac cagtttgcag cgcagcggca tcgtcgttac
     9541 aacatacgaa gggcaggaac cgactcccga agatgtgtta atcactgacg aggtagtgag
     9601 taaaaaatgg cagggtagag cggtagtgac cttctgtcgt cgccatattg gtattccgct
     9661 ggagaaagcg ccaggggagt gggtacacag tgtggctgct ccgcatgagc taccggcatt
     9721 gttggcgcgt atttatttga tcgagatgga gagcgacgat cctgctaacg ctctgccgtc
     9781 gacggacaaa gcggtcagcg ataacgacga tatgatgatt ctggtcgtgg atgatcatcc
     9841 gattaaccga cgtctgctgg cagatcagtt gggatcgttg ggctatcaat gtaaaaccgc
     9901 gaatgatggc gtcgatgcgc ttaatgtact tagcaagaat catattgata tcgtgcttag
     9961 cgacgtcaac atgccaaata tggatggtta ccgcttgacg caacgcattc gtcagttggg
    10021 actgacgttg ccggtaatcg gagtaactgc taatgcgttg gctgaagaga agcagcggtg
    10081 tctggagtcc ggtatggaca gctgcctgtc gaagccggta acgctggatg tgataaaaca
    10141 gacgctgacg gtatatgccg agagggtcag gaaatcgcgg gaatcgtagg acggataagg
    10201 cgtttacgcc gcatccggca ttcagtgcaa atgccagatg cgacgctgac gcgtcttatc
    10261 tggcctactt taatgtaatc tgttgaaata atgggaatcg taggccggat aaggcgttta
    10321 cgccgcatcc ggcattcagt gcaaatgcca gataagacac taacgcgtct tatctggcct
    10381 acaggtgatt agtctttatc tgccggactt aacgtcacag aagagagata attcagcagg
    10441 gcgatatcgt tctcgacacc cagcttcatc atcgcagatt tcttctggct actgatggtt
    10501 ttaatactgc ggttcagctt tttagcgatc tcggtcacca ggaagccttc cgcaaacagg
    10561 cgcagaactt cactctcttt tggcgagaga cgcttgtcac cgtaaccacc agcactgatt
    10621 ttttccaaca ggcgagaaac gctttccggg gtaaatttct tccctttctg cagcgcggcg
    10681 agagctttcg gcagatcggt cggtgcacct tgtttcagca cgatcccttc gatatccaga
    10741 tccaataccg cactaagaat cgccgggttg ttgttcatgg tcagaacaat aatcgacagg
    10801 cttgggaaat ggcgcttgat gtacttgatt aaggtaatgc catcgccgta cttatcgcca
    10861 ggcatggaga gatcggtaat caacacatgc gcatccagtt tcggcaggtt gttgatcagt
    10921 gctgtagagt cttcaaattc gccgacaaca ttcacccact caatttgctc aagtgatttg
    10981 cgaataccga acaagactat cggatggtca tcggcaataa ttacgttcat attgttcatg
    11041 taataggcta ccttgctaca gcaagctctt gacataactg tcaatgtcgc tgatgtattt
    11101 ttctattcct ggaacatcct tctcacgaat cagatgttcc agcgtttcac ataactgctt
    11161 gccgggtacc agatttagca tggcaaatac gcctttaaga cgatgagccg tttgggctaa
    11221 cgcagcaaag tcactggttg ctgcttcagt atacagcctc ttaacatcat ccggtactgt
    11281 gtctacaaag agcgcataat agccgctggc atggagttgc gcattttcat ctccgcccag
    11341 aggcgattct gtcacctctt cctgcgccag ttgcacttca attaattgta agaccgcttc
    11401 ctgcatagcg ttgctcatat tgaagttgac gcacaattga ccaggcccaa tttcccgtac
    11461 gccagactca tcatcgctta aaagcaagcc agaggcagta agattagacg gattatccgt
    11521 taaaaagata tcataatctt gactaattaa tctttcatcg ggtgtgatac aggttgcacc
    11581 ccaattttct aactggcgag tgacaatatt ccgaatttct gccgaagtaa catccaccat
    11641 tacgcagaca tcatccagta aacgctcttc ttcctcttca acttccgggt cagcggcgag
    11701 cattttgata tgcacagagt agcgtgtacc aagcccatcc cgcgttttga tgtttaaatg
    11761 accgcccagt ttacgcgcca gttgatcgct cagccagaat gccagcgggt ccgccttgcc
    11821 atagcgatcg ttttgggtct ggttgataaa cgggaagtgc aaattatcca tttcatgaat
    11881 acttacgcct tctccggtgt ccagaatgcg gaatgtcagg cggtcttcag aggactcatc
    11941 ctgatcaacc tcaagggtga tttttcccaa ttgcgttgag gtcacggcat attgcatcag
    12001 tagcagcaaa atacgtcgta aggcatcgcg atcgccgcgg cgcatatcgt gtgctttcag
    12061 atgattgtta atcagcagtt gcagaccttt acgcttgatg gcaggcaaca ctgaaggcac
    12121 aacttcatca attaaatcct gcacggagaa cagcaccgtc tcacttttcc agctatcatc
    12181 cgcaagcatg ttcgctaact gtatttcatc aaccagccgc accagcacat ctgcctgatt
    12241 cgccagttgt ttgctttccg gggcgttgag tttagccgcg ctctccgcca gggactgtgc
    12301 aggttctttc agcgcatcgc caatgttttt cataaaggtc atccggctct gctggttttt
    12361 ctcatacaga cgctgcgcct gcttgagttt cttgtttacc agcacttcgc gatcctgatc
    12421 gcgaataatg aaaatttgtg tgcgcggcgc gacctggctg cggaacatgc ggatctcata
    12481 cagctcgtta ttgatcgtcg cctgaataat cccctgatgc tgttccgcca tggtggtgat
    12541 gttttgcaga ttcaaatgcg gcagcaaatg atcggcaatt ttgttactta tgacagtgcg
    12601 gttcgattcc tgatcgtgaa ccagcaggcc gagcggcagc agtgagacta tctcttcatt
    12661 aattgcccgt aaaatgcgca attcgttatt gaccgccgtg ctgggtagac tttctgtact
    12721 gcggctggag aaatggcgga atgtggtata gccaaataac gccagcgcca gcaaaccgat
    12781 gttcagcagc agtggcagca gaatgttttg caacgtatcc agcaataagg tgccataagg
    12841 aacctgccag accaggcgca tatcggtaga gttgagtgcc gaggagattt caatcttcgt
    12901 actgttaaag tggatactga cgctatccgt cccttctttc tcattatcat tgtttcccgt
    12961 tgccgtcgcg tctggctcaa ggcggaaact gtccagcggc atacccggtg ggatcaaatc
    13021 attaatcggc agatcaaaag ccacgaccgt tgccagatgt cctggctggt taaaggtagt
    13081 acgcaaggta aagtaatgac cgttctgcca ggccaggcgg cgcatgttag aaaagctttc
    13141 gcgttcatcg agggcgttgg cctgttgcaa catctctgca cgacgtgaat caacaatgtc
    13201 gctgacggtc gattctttaa atccggaggt gagatcttta aggggtaggg ttgagatcag
    13261 caccagactg ttatcctgac cattcaggta atacatcgac cacggtacat tttctgcgcc
    13321 ccacaatgta tccagataag tggacatccg ctgagtcatc tcaagcgttg agttgtcgtg
    13381 agagccaaag attaacgctt ccgttttgcg gcgtggtttc tccagatagt agacatcctg
    13441 tttcaggctc gtctcttgta aaccttcgcc ggaggagggg gaggtcgtcg cggcaatgtt
    13501 gtcgtagatc tgccaggtca cgtaacgcca ggtatcgacg cgcttttgga tagcgtgggt
    13561 aatgtcgaca atctggtaac ttttatcttt cagccatgcg ttaacggcgc tttgtaccat
    13621 tacacccatc gtcaccagta acacaatgat caacagtaaa aagaagcggg taatgctccc
    13681 cggtaggagt gaaaagcggg tcgtggccgt tgtctctttc tgacgcattc gtgtttatga
    13741 cctgttaaaa cttcgcgaag tgatagggca gtataaaggg tacagtgtga aattccagac
    13801 tcttacctga ttgatgattc tctattctcc cgtgatattt atcatcctgc attgggggaa
    13861 atagatgtac aagcgccatt ttgctgtaca aataacctac aaaaagccca acgaaaataa
    13921 ttgcaaataa acaaagattg ctggaaatta tgctgatgtt aattatttgt gaaatagtta
    13981 acaagcgtta tagtttttct gtggtagcaa agaataatga aaagtgtgta aagaagggta
    14041 aaaaaaaccg aatgcgaggc atccggttga aataggggta aacagacatt cagaaatgaa
    14101 tgacggtaat aaataaagtt aatgatgata gcgggagtta ttctagttgc gagtgaaggt
    14161 tttgttttga cattcagtgc tgtcaaatac ttaagaataa gttattgatt ttaaccttga
    14221 attattattg cttgatgtta ggtgcttatt tcgccattcc gcaataatct taaaaagttc
    14281 ccttgcattt atattttgaa acatctatag cgataaatga tacatcttaa aagttttagt
    14341 atcatattcg tgttggatta ttctgcattt ttggggagaa tggacttgcc gactgattaa
    14401 tgagggttaa tcagtatgca gtggcataaa aaagcaaata aaggcatata acagagggtt
    14461 aataacatga aagttaaagt actgtccctc ctggtcccag ctctgctggt agcaggcgca
    14521 gcaaacgctg ctgaagttta caacaaagac ggcaacaaat tagatctgta cggtaaagta
    14581 gacggcctgc actatttctc tgacaacaaa gatgtagatg gcgaccagac ctacatgcgt
    14641 cttggcttca aaggtgaaac tcaggttact gaccagctga ccggttacgg ccagtgggaa
    14701 tatcagatcc agggcaacag cgctgaaaac gaaaacaact cctggacccg tgtggcattc
    14761 gcaggtctga aattccagga tgtaggttct ttcgactacg gtcgtaacta cggcgttgtt
    14821 tacgacgtaa cttcctggac cgacgtactg ccagaattcg gtggcgacac ttacggttct
    14881 gacaacttca tgcagcagcg tggtaacggt ttcgcgacct accgtaacac tgacttcttc
    14941 ggtctggttg acggcctgaa ctttgctgtt cagtaccagg gcaaaaacgg tagcgtaagc
    15001 ggcgaaggca tgactaacaa cggtcgtgaa gcactgcgtc agaacggcga cggcgttggc
    15061 ggatctatca cttatgatta cgaaggcttc ggtatcggtg ctgcagtttc cagctccaaa
    15121 cgtactgatg atcagaacag cccgctgtac atcggtaacg gcgaccgtgc tgaaacctac
    15181 actggtggtc tgaaatacga cgctaacaac atctacctgg ctgctcagta cacccagacc
    15241 tacaacgcaa ctcgcgtagg ttccctgggt tgggcgaaca aagcacagaa cttcgaagct
    15301 gttgctcagt accagttcga ctttggtctg cgtccgtccc tggcttacct gcagtctaaa
    15361 ggtaaaaacc tgggtcgtgg ctacgacgac gaagatatcc tgaaatatgt tgatgttggt
    15421 gcgacctact acttcaacaa aaacatgtcc acctatgttg actacaaaat caacctgctg
    15481 gacgacaacc agttcactcg tgacgctggc atcaacactg ataacatcgt agctctgggt
    15541 ctggtttacc agttctaatc tcgattgata tcgaacaaag ggcctgcggg cccttttttc
    15601 attgttttca gcgtacaaac acagtttttt ggtgtactct tgcgaccgtt cgcatgagga
    15661 taatcacgta tggaaataag ctttacccgc gtggcactgc tggctgccgc gctcttcttt
    15721 gttggttgcg atcaaaaacc acaaaccgcc aaaacccacg ctactgaagt taccgttctt
    15781 gaaggcaaaa ctatgggtac cttctggcgt gccagcatcc cgggcattga cgccaaacgc
    15841 agtgccgaac ttaaagaaaa gattcagacc cagctggacg ccgacgatca gctgctttcg
    15901 acctataaaa aagattccgc gctgatgcgc tttaacgatt cgcaaagttt atcgccgtgg
    15961 ccggtaagtg aagcgatggc cgatatcgtc accacctcgc tgcgcattgg cgcaaggacc
    16021 gatggtgcga tggatataac cgtcgggccg ctggtgaatc tgtggggctt tggcccggaa
    16081 caacagccgg ttcaaattcc gagccaggaa cagatcgatg cgatgaaagc caaaaccggc
    16141 ttacagcacc tgacggtcat taatcagtcg catcagcaat atctgcaaaa agacctaccg
    16201 gatttatatg tcgatctctc caccgtcggt gaaggttatg cggcggatca cctggcacgc
    16261 ttgatggagc aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc
    16321 cgtggtatga acggtgaagg cctgccgtgg cgggtagcga ttcaaaaacc aaccgataaa
    16381 gaaaacgcgg ttcaggctgt ggtggatatc aacggtcatg gtattagcac ctctggcagt
    16441 taccgtaact attacgaact ggacggcaaa cgtctttctc atgttatcga tccgcaaacc
    16501 gggcgtccca tcgaacacaa tctggtatcc gtgacggtga ttgctccgac ggcgctggaa
    16561 gccgatgcct gggatactgg cttgatggta ctcgggccgg agaaagccaa agaggttgtt
    16621 cgccgggaag ggctggcggt ttatatgatc accaaagaag gcgatagctt taaaacctgg
    16681 atgtcaccac agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc aagattgctg
    16741 gtttttgcgt gatggtgacc gggcagacta aaggctatcc ttaaccaggg agctgattat
    16801 gaaaaacgcc acatgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgaccc
    16861 gaatgccgac agcgaattcg ttttcgccgt gcgcaccaca ggcatctttt gccgtccgtc
    16921 ttgccgcgcc agacatgcct tgcgggaaaa tgtctccttc tacgcaaatg cctgcgaggc
    16981 actcgccgcc ggatttcgcc cctgcaaacg ctgccaacct gataaagcca atgcccagca
    17041 acatcggttg gataaaatca cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac
    17101 gctggaagcc ttagccgacc aggtggcgat gagtccattt catctacatc ggttgtttaa
    17161 agcgactacc ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg
    17221 cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt cttaacgccg gattccccga
    17281 cagcagcagt tactatcgca aagctgacga aacgctgggc atgacggcta aacaattccg
    17341 tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc tgggtcgttg
    17401 cctggtggca gaaagcgagc gggggatttg cgcgatattg ctgggcgatg atgacgcgac
    17461 actaatcagc gagttgcagc agatgtttcc cgctgccgac aacgcgcctg ccgatctgat
    17521 gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat caacgcgata cgccgctgac
    17581 gttaccgctg gacattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac
    17641 gataccttgc ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa
    17701 agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag ctggctatca taataccctg
    17761 tcatcgggtg gtccgtggtg atggcacact ttccggttac cgctggggcg tgtcgcgtaa
    17821 agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg taatgttgga tctgtttgcc
    17881 gatgctgaac cgtggcaaga gccactggcg gctggagcgg taattttgca ccgttttgct
    17941 tttaacgctg cggagcaact gatccgcgat attaatgacg ttgccagcca gtcgccgttt
    18001 cgccagatgg tcacccccgg gggatatacc atgtcggtgg cgatgaccaa ctgtgggcat
    18061 ctgggctgga cgacccatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat
    18121 aaaccgtggc ccgccatgcc acaaagcttt catgatttat gtcaacgtgc ggctacggcg
    18181 gcgggctatc cggatttcca gccagatgct tgtcttatca accgctacgc tcctggcgcg
    18241 aaactgtcgc tgcatcagga taaagacgaa ccggatctgc gcgcgccgat tgtttctgtt
    18301 tctcttggct tacccgcgat ttttcaattt ggtggcctga aacgtaatga tccgctcaaa
    18361 cgtttgttgt tggaacatgg cgatgtggtg gtatggggcg gtgaatcgcg gctgttttat
    18421 cacggcattc aaccgctgaa agcggggttt catccactca ccggcgactg ccgctacaac
    18481 ctgacattcc gtcaggcagg taaaaaagaa taaaaataag aattattatt gctgtgcgcg
    18541 cgaagatgtt taaactgcgg gctgtaattc attgtccggg ttttctgcat ggaacttctt
    18601 gtacttgtct ggcggcagta tcgctggcca tttatcagtg tgatggcgct aagcctcgcc
    18661 agtgcggcat taggcattgg cttaattgct tttatcaatc agcgccttat cgaaacggcg
    18721 gataccagtc tgctggtatt gccggagttt ctgggattat tgctgctgtt gatggcagtc
    18781 actctcggat cgcaactggc gctcaccact ttggggcatc acttcgttta ccgactgcgt
    18841 agtgaattta tcaagcggat tctggatacc aacgtcgagc gcattgaaca actcggtagc
    18901 gcctcgttgc tggcgggatt aaccagcgat gtgcgcaata tcaccattgc ttttgtgcgt
    18961 cttccggaac tggtgcaggg gatcattctc actatcggtt ccgcggcgta tctgtggatg
    19021 ctgtcgggca aaatgttgct ggtaacggct atctggatgg cgatcaccat ctggggtggt
    19081 tttgtactgg tggcgcgggt gtacaaacat atggcgaccc tgcgtgaaac cgaagacagg
    19141 ctgtatacgg attttcaaac tgtactcgaa gggcgcaaag agctgactct gaaccgggaa
    19201 cgcgccgagt atgtgtttaa caacctctac attcctgatg cgcaagagta tcgccaccat
    19261 attatccgcg cagacacctt ccatcttagc gccgtgaact ggtcaaacat catgatgttg
    19321 ggcgcaatcg gcctggtgtt ctggatggcg aacagtctcg gttgggctga taccaacgtt
    19381 gccgcgacct attcgttgac gcttttattc ctgcgtacgc cgctgctttc ggcggttggc
    19441 gcattgccga cgctgctgac ggcgcaggtg gcgtttaaca agctgaacaa attcgcgctc
    19501 gcgcctttca aagcagagtt tccgcgcccg caggcgtttc ccaactggca aacgctggag
    19561 ctgcgtaacg tgacgtttgc ttatcaggat aacgcgtttt ccgttggtcc gattaatctc
    19621 accatcaaac gtggcgagct gctgtttctg attggcggca acggtagcgg aaaatcgacg
    19681 ctggcgatgt tgttgacggg cttgtatcag ccacaaagcg gcgaaatctt gctggatggc
    19741 aaacctgtca gcggcgaaca accggaagat tatcgcaaac tgttttcggc agtgtttacc
    19801 gatgtctggc tgtttgatca actgctgggg ccggagggta aacccgctaa cccgcaactg
    19861 gttgagaagt ggctggcgca gctgaaaatg gctcataagc ttgagttaag caacgggcgt
    19921 attgttaacc tgaagttatc aaaagggcag aaaaaacgcg tggcgctgtt gctggcgctg
    19981 gcagaagaac gcgatattat cctgctggat gaatgggcgg cggatcagga tccacacttc
    20041 cgtcgtgagt tttatcaggt gttgctgccg ctgatgcagg agatgggtaa aactattttc
    20101 gccatcagtc atgatgatca ttactttatc cacgccgacc gcctgctgga aatgcgcaac
    20161 gggcaactta gcgagctgac gggcgaagag cgcgatgccg cttcgcgtga tgccgttgcc
    20221 cggacggcat aatattacga cgctgacctg gcggactgct gccgtcaggt caatatgcta
    20281 aaaatccctt catttctaat aaccctataa ttaatcaacg aaattataat gtttctaaaa
    20341 ttagaatata atttataaac attatttaaa tgttgttact taagtgttac cgttgatgcc
    20401 gcgcaaatct cacctgcaat aaagcgacta aaagtaaggc attaacaaga tgaaaaaagt
    20461 gactgccatg ctcttctcga tggccgtggg gcttaatgcc gtttcgatgg cggcaaaagc
    20521 gaaagcgtcc gaggagcagg aaactgatgt actgttgatt ggcggcggca ttatgagcgc
    20581 cacgttgggg acctatttac gcgagctgga gcctgaatgg tcgatgacca tggtggagcg
    20641 cctggagggt gtcgcgcagg agagttcgaa cggctggaat aacgccggaa ccgggcattc
    20701 tgcactgatg gaactgaact acaccccgca aaacgccgat ggcagcatca gtattgaaaa
    20761 agcagtcgcc attaacgaag catttcagat ttcccgccag ttctgggcgc accaggttga
    20821 gcgcggcgtg ctgcgtactc cgcgttcatt tatcaatacc gttccgcata tgagctttgt
    20881 ctggggcgag gataacgtta atttcctgcg cgcccgttac gccgcgttgc aacaaaactc
    20941 gctgtttcgc ggtatgcgtt actctgaaga tcacgcgcag atcaaagagt gggcaccgtt
    21001 agtgatggaa gggcgcgatc cacaacagaa agtagcggca acacgcacag aaattggcac
    21061 tgatgtgaac tatggcgaga tcacccgcca gttaattgct tccttacaga agaaatctaa
    21121 cttctcgctg caactcagca gcgaagtccg cgccctaaag cgtaatgacg ataacacctg
    21181 gaccgttacc gttgccgatc tgaaaaacgg cactgcacag aacatccgcg ccaaatttgt
    21241 ctttatcggc gcgggcggtg cggcgctgaa gctattacag gaatcgggga ttccggaagc
    21301 gaaagactac gccggtttcc cggtgggcgg acagttcctt gtttcggaaa acccggacgt
    21361 ggttaatcac catctggcga aggtttacgg taaagcatcc gttggcgcac caccgatgtc
    21421 ggttccgcat atcgataccc gcgttctgga cggtaaacgc gtagtgctgt ttgggccatt
    21481 tgccaccttc tcaaccaaat tcctcaaaaa cggttcattg tgggatctga tgagttccac
    21541 caccacctct aacgtgatgc cgatgatgca cgtcgggctg gataatttcg atctggtgaa
    21601 atatctggtg agtcaggtga tgttgagtga agaggatcgt tttgaagcgt tgaaagagta
    21661 ctatccgcag gcaaaaaaag aggactggcg tttgtggcaa gcggggcagc gcgtgcagat
    21721 tatcaagcgt gatgccgaga aaggtggcgt actgcgtctg ggtactgaag tggtcagtga
    21781 ccagcaaggg accattgccg cgctgctggg ggcatcgcca ggggcgtcaa ccgccgcgcc
    21841 gattatgttg gatctgctgg aaaaagtatt tggcgatcgc gtttccagcc cgcaatggca
    21901 ggctacgttg aaagcgatcg ttccgtctta tggacgcaag ctgaacggtg atgtagcggc
    21961 aacagaacgc gagttgcagt acaccagcga agtgctgggg ctgaaatacg acaagccgca
    22021 ggcagcagat agtacgccaa aaccgcagtt gaaaccgcaa cccgttcaaa aagaagtggc
    22081 ggatattgcg ttgtaatgat gcgccacatc cggcatggta tgccggatgt ggcgtatgct
    22141 gataagacgc gccagcgtcg catcaggcac cggctcgggc gttagatgtc ggatgcggcg
    22201 tgaccgcctt attcggccta cggggtgccg cacttcacag cattttagcg aactaccgcg
    22261 ttgtcaattt tctcttccgc cttccagacg cggtacttca catctacatt gtctggcgta
    22321 tacaccacga tcggcagctt gctgttgtaa cgcagcattc cagcatcgcc cagatacgcg
    22381 gtgacaaatt tcttctcttt cttgccatcc gggcaggcca tcatcgtgga aaccggcgag
    22441 ctaactttat caaagacata ataatcatag ccccagcctt ccagcgtttt gctttccagc
    22501 ttcccgccga gacgatgcaa attgcaatcg acttccagcg tctgaccgat taacagttct
    22561 actttcaggg tagattcatc ttcttgcggg gttaactgaa tcacctgacg cttcatccct
    22621 ttttcagctt gtggataagg cgcgattttt tccagtggct ggacgctttc tgccgcccag
    22681 gcggaagtgg tagcgaaagc ggcaaacaat actgcaggta gaatggtctt catttttttg
    22741 ctttccttct tcaatttata ggttttcacc gatgatgtcg ctgaaggtta acatattcat
    22801 cagatttatt aatttgcttt atgaataact taaagaacat tcagacaatt ttacctgctc
    22861 ttttagtgtt aaattctgat gagggagcaa accagcagaa atatctctgg tgaattaatc
    22921 gctatataaa tatgtttata accatttgaa atgtgagcaa aagcccgttt ttgccacgct
    22981 tcccgccact cttttgatcc tgctacaggt tttaccccga tcggggtatg catctttgac
    23041 acatccttta atatcttagc ggctataaaa atggcttatt aattatgcgg tttatttggt
    23101 cgctctcaat tttcagagcg cgttaatgat ggaaggtcaa tgtgaagatt gatgcatccc
    23161 gtcggggcat actcactggt cgctggcgca aagccagtaa cggtatccgt ccgccctggt
    23221 ccggtgatga atctcatttt ctgacccatt gcacccgctg cgacgcctgt attaatgctt
    23281 gtgaaaacaa cattctgcaa cgcggcgcgg gcggctatcc gagcgttaat ttcaaaaata
    23341 acgagtgcag cttctgttac gcctgcgcgc aggcttgccc cgaatcgcta ttttctccgc
    23401 gccacaccag ggcctgggat ttgcagttca ccattgggga cgcgtgcctg gcgtatcagt
    23461 cagttgaatg ccgccgctgt caggatagct gtgagccaat ggcaattatc tttcgcccga
    23521 cgctctccgg gatctaccag ccgcaactta atagccaact ttgcaacgga tgtggagcat
    23581 gtgcagccag ctgtccggta tcagccataa ctgcggagta tctccatgca cactaactgg
    23641 caagtttgca gcctggtcgt gcaggccaaa agcgaacgaa tttcagacat cagcacccaa
    23701 ctgaacgcct ttcccggctg tgaagttgct gtcagcgacg cgccgagcgg tcagttgatt
    23761 gtggtggtgg aagcagaaga cagcgaaacg ctgatccaaa ccattgagtc agtacgcaac
    23821 gtagagggcg tgctggcggt gtcgctggtt tatcaccagc aggaagagca aggtgaggaa
    23881 acaccatgaa actcagtcgt cgtagcttta tgaaagctaa cgccgttgcg gccgctgcgg
    23941 cggctgccgg tctcagcgtg ccgggcgttg cccgcgccgt tgttggtcag caggaagcca
    24001 ttaaatggga taaagcgccg tgccgtttct gcggtactgg ttgcggcgtt ctggtcggaa
    24061 cgcagcaggg gcgtgtggtg gcctgtcagg gcgacccgga cgcaccggtt aaccgtggcc
    24121 tgaactgcat taagggctat ttcctgccca agatcatgta cggtaaagac cgtttgacgc
    24181 agccgctgct gcgtatgaaa aacggtaaat atgacaaaga aggcgaattt accccaatca
    24241 cctgggatca ggccttcgat gtgatggaag agaaattcaa aaccgccctg aaagaaaaag
    24301 gaccggaatc gatcggtatg ttcggttctg gtcagtggac tatctgggaa ggttatgccg
    24361 cgtccaagct gttcaaagcg ggcttccgtt cgaacaacat cgacccgaac gcacgtcact
    24421 gtatggcgtc ggcagtagtt ggctttatgc gtacctttgg tatggatgag ccgatgggct
    24481 gctatgacga catcgagcag gctgacgcgt ttgtgctgtg gggcgctaac atggcggaga
    24541 tgcacccgat cctctggtca cgcatcacta accgtcgtct ctctaaccag aacgtcaccg
    24601 tggcggtgct ttctacctac cagcatcgta gcttcgagct ggcggataac ggcatcatct
    24661 ttacgccaca gtctgacctg gtgatcctga actacatcgc caactatatc attcaaaaca
    24721 atgcgataaa tcaggacttc ttcagcaagc acgttaacct gcgcaaaggg gcgacggaca
    24781 tcggctacgg tttacgtccg acccatccgc tggaaaaagc agcgaagaat ccgggttctg
    24841 acgcctccga accgatgagc tttgaagatt acaaagcctt cgttgccgag tatacgctgg
    24901 aaaaaactgc cgaaatgacc ggcgtgccga aagaccagtt agaacaactg gcgcagctgt
    24961 atgccgatcc gaacaagaaa gtcatctcct actggacgat gggcttcaac cagcatactc
    25021 gtggcgtgtg ggccaacaac ctggtctaca acctgcacct gctgaccggc aaaatttccc
    25081 agccgggttg cggtccgttc tccctgaccg ggcagccttc cgcgtgtggt actgcgcgtg
    25141 aagtgggcac ctttgctcac cgtctgcctg cggacatggt ggtgactaac gagaaacacc
    25201 gtgatatttg cgagaagaag tggaatatcc cgagcggcac cattccggcg aaaatcggtc
    25261 tgcacgcggt ggcacaagac cgtgcactga aagacggcaa gctgaatgtt tactggacca
    25321 tgtgtaccaa caacatgcag gccgggccga acattaatga agagcgtatg ccgggctggc
    25381 gtgatccgcg caacttcatc atcgtctccg atccgtatcc gacagtcagt gcgctggcag
    25441 ccgacttgat cctgccgacc gcaatgtggg tagagaaaga gggcgcttac ggtaacgccg
    25501 aacgccgtac tcagttctgg cgtcagcagg tacaggcgcc aggcgaagcg aaatcggatc
    25561 tctggcagtt agtccagttc tcccgccgct tcaaaactga agaagtatgg ccggaagagc
    25621 tgctggcgaa gaaaccggaa ctgcgtggca aaacgctgta cgaagttctg tatgccaccc
    25681 ccgaagtgag caaataccct gtatccgaac tggcggaaga tcagctgaac gatgaatccc
    25741 gcgagctggg cttctatctg caaaaagggc tgttcgaaga gtacgcatgg tttggtcgcg
    25801 gtcacggtca cgatctggca ccgttcgatg actaccacaa agcgcgcggt ctgcgctggc
    25861 cggtggtgaa cggtaaagaa acgcagtggc gttacagcga aggtaacgac ccgtacgtga
    25921 aagcgggcga aggctataag ttctacggta aaccggatgg caaagcggtg atcttcgcac
    25981 tgccgttcga accggcggcg gaagcaccgg atgaagagta cgacctgtgg ctctctaccg
    26041 gacgcgttct ggagcactgg cacaccggca gtatgactcg ccgtgtgccg gaactgcacc
    26101 gcgccttccc ggaagcggtc ttgtttattc acccgctgga tgcgaaagcg cgcgatctgc
    26161 gccgtggcga caaagtgaaa gtggtttctc gccgtggcga agtgatctcg attgttgaaa
    26221 cgcgcggtcg taaccgtccg ccacagggcc tggtgtacat gccgttcttc gacgccgcac
    26281 agctggttaa caaactgacg ctggatgcga ccgatccgct ctcgaaagag acggacttca
    26341 agaagtgcgc agtcaaactg gagaaggtgt aagccattat gtcccggtca gcgaaacctc
    26401 aaaatggtcg ccgccgcttt ctgcgcgatg tcgttcgcac agcaggcggg ctggctgccg
    26461 tgggtgtggc gctggggtta caacagcaaa ccgcacgcgc atctggcgtg cggttgcgcc
    26521 cgcccggagc cataaacgag aacgcctttg ccagtgcctg tgtgcgttgc ggtcagtgtg
    26581 ttcaggcttg cccttacgac accttaaaac tggcgacgct ggcctctggt ctgtcggcgg
    26641 gcacgccata ttttgtcgca cgggatattc cttgcgaaat gtgtgaggac attccgtgcg
    26701 ccaaagtgtg cccaagcggt gcgctggatc gtgagattga atcgatcgac gacgcgcgga
    26761 tggggctggc ggtactggtg gaccaggaaa actgtctcaa ctttcagggg ctgcgctgcg
    26821 atgtttgtta tcgcgaatgc ccgaaaattg atgaggccat caccctggag ctggagcgca
    26881 acacgcgtac cggtaagcac gcccgctttc tgccgacggt tcacagcgac gcctgtaccg
    26941 gttgcggtaa atgcgaaaaa gtatgcgtgc tggaacaacc ggcaatcaag gtgttaccgc
    27001 tgtcactggc gaaaggggag ttaggtcacc attaccgctt cggttggctg gaggggaaca
    27061 atggcaaatc gtaaacgtga cgccgggcgc gaggcgctgg agaaaaaagg ctggtggcgc
    27121 agtcaccgtt ggctggtgtt acgtcgcctt tgtcagttct tcgtgctggg gatgtttttg
    27181 agcggtccat ggtttggtgt gtggatcttg cacggcaact acagcagtag cctgttattc
    27241 gacaccgttc cgctgaccga tccgctgatg acgctgcaaa gtcttgccag tggtcatctg
    27301 cctgccacgg tggcgttgac cggtgcggtg attatcaccg tgctctacgc cctggcggga
    27361 aagcgattat tttgcagctg ggtctgcccg ctgaacccga ttaccgactt agcgaactgg
    27421 ttacgcaggc gctttgacct caatcagtct gcgacgatcc ctcgccacat tcgctacgtg
    27481 ctgttggtgg tcattctgct gggatcggcc ttaactggca cgctcatctg ggaatggatc
    27541 aaccccgtat ctctgatggg gcgcagcctg gtaatgggct tcggcagcgg cgcgctgctt
    27601 attctcgcgc tgtttttatt tgatctactg gtcgttgaac acggctggtg cgggcacatt
    27661 tgtccggttg gcgcgctgta tggcgtgctg ggtagcaaag gtgtgattac cgttgcggcc
    27721 accgatcggc agaaatgtaa ccgctgtatg gattgttttc atgtttgccc ggaaccgcat
    27781 gtgctacgtg ccccggtgct ggatgaacaa agcccggtgc aggtcaccag ccgcgattgc
    27841 atgacgtgcg gtcgctgcgt ggatgtctgt tctgaggatg tatttacaat aactacacga
    27901 tggagttcgg gagcgaaatc atgaaaagcc atgacctgaa gaaagcgctg tgtcaatgga
    27961 cggcgatgct ggccctggtg gtaagcggcg cggtttgggc ggctaatggc gtcgatttta
    28021 gccagtcgcc ggaagtatcc gggacacagg aaggggccat tcggatgcca aaagagcagg
    28081 aacggatgcc gctgaactat gtgaatcagc cgccaatgat cccgcatagc gttgaaggtt
    28141 atcaggtaac gaccaatacc aaccgctgct tgcagtgcca cggtgtcgaa agttatcgca
    28201 ccactggcgc gctgcgtatc agtcctactc actttatgga cagcgacggc aaagtgggcg
    28261 cggaagtggc tccgcgtcgt tatttctgtc tgcaatgtca cgtaccgcag gccgataccg
    28321 cgccaatcgt ggggaatacc tttaccccat caaaaggtta cgggaaataa gaggttatta
    28381 tgggaaattc tgaccgtaag cctggtctga ttaagcgcct gtggaaatgg tggcgtaccc
    28441 ccagccgtct ggcgctgggg acgctgctgt tgatcggttt tgttggcggc atcgtcttct
    28501 ggggtggctt taacaccggg atggaaaaag ccaataccga agagttctgc attagctgcc
    28561 acgaaatgcg caacacggtg tatcaggaat acatggattc cgtgcactac aacaaccgta
    28621 gcggcgtccg tgcgacctgt ccggattgtc acgttccgca cgagtttgtg ccgaagatga
    28681 tacgcaagct caaagcaagt aaagagctgt atggtaaaat ttttggcgtt attgacacgc
    28741 cgcagaaatt tgaagctcat cgtctgacga tggcacagaa tgagtggcgg cgcatgaagg
    28801 acaataactc gcaggagtgc cgtaactgtc acaacttcga gtatatggat acaaccgccc
    28861 agaaatcggt tgccgcgaag atgcatgacc aggcggtgaa agatgggcaa acctgtattg
    28921 attgccataa agggatagcg cacaagctgc ccgatatgcg tgaagtcgag ccaggttttt
    28981 aacagggtta ttgcgtgggt atgcttgaag ccagagagtt actttgtgag cgggatgaac
    29041 gaaccttatt tagtggcttg tcatttacgc tgaacgcagg agagtgggta caaatcaccg
    29101 gtagcaacgg cgcggggaag acaacgcttc tccgtttgct gacggggttg tctcgccctg
    29161 acgcaggcga ggttctctgg caagggcagc ccttgcatca ggtacgcgac agctaccatc
    29221 aaaacctgtt atggataggc catcagccgg ggatcaaaac ccggctgacg gcgttagaaa
    29281 atctgcactt ttatcatcgc gatggcgata ccgcacaatg tctggaagcc ctggcgcagg
    29341 ccgggcttgc cggattcgaa gatattcctg taaatcagct ctcggccggg caacaacgcc
    29401 gcgtcgcttt agcgcgtctg tggctgaccc gtgccacgtt atggatcctc gacgagcctt
    29461 ttaccgcgat tgacgttaac ggcgtcgatc gtctgaccca gcgtatggcg cagcatacgg
    29521 agcagggggg gattgtgatt ctgactaccc accagccgct caacgttgct gaaagtaaaa
    29581 ttcgccgcat ttcactgacg caaacgaggg ccgcatgatg ttctggcgca ttttccgtct
    29641 tgagctgcgt gtagcgtttc gccatagcgc cgaaatcgcc aacccgctgt ggttcttcct
    29701 gattgtaatt accctttttc cgctcagtat cggtccggag ccgcaactgc tggcgcgtat
    29761 tgcaccgggc attatctggg ttgctgcgct gctttcatcc ttgctggcgc tggaacgact
    29821 gttccgtgac gatttgcagg acggcagtct tgaacaattg atgttgttgc cgttaccctt
    29881 gcccgccgtt gtgctggcga aggtgatggc gcactggatg gtaagcggtc tgccgttact
    29941 cattctttcg ccactggtag caatgctact gggaatggat gtttatggct ggcaagtgat
    30001 ggcgctgacg ctgctgctgg gaacgcctac gcttggcttt ctcggtgcac cgggcgtggc
    30061 gctgacagtg ggacttaagc gcggtggtgc gctgctcagc atactggtgt taccgctgac
    30121 tatcccatta ctcatcttcg ccaccgccgc gatggacgcg gcttccatgc atttgcccgt
    30181 tgacgggtat ctggcaattt taggcgcgtt gctggcaggc accgcgacat taagtccttt
    30241 tgcgacggcg gcagcgttac gaatcagcat tcaataacgc tggattactg atttttgttc
    30301 gagtctggta tcgaaactat gtggaaaaca ctgcatcaac tggcgatccc accacggctg
    30361 tatcaaatct gtggctggtt tataccgtgg ctggcaattg ccagtgtggt cgtgcttacc
    30421 gtcggctgga tctggggatt cggctttgct ccggctgatt atcagcaggg aaatagctac
    30481 cgcattatct acctgcatgt gcctgcggcg atctggtcga tgggcattta tgcatcaatg
    30541 gcagtggcag cgtttattgg ccttgtctgg cagatgaaaa tggccaacct ggcggtggcg
    30601 gcgatggccc ccattggtgc cgtgtttacc tttattgccc tggttaccgg ctctgcatgg
    30661 ggaaaaccga tgtggggcac ctggtgggta tgggatgcac gtctgacttc tgaactggtg
    30721 ctgctttttt tgtatgtggg tgtgattgcc ctgtggcacg ccttcgacga ccgccgtctg
    30781 gcgggccgtg cggcaggtat cctggtgctg attggcgtgg tgaatctgcc gattattcat
    30841 tactccgtgg agtggtggaa caccctgcat cagggatcaa cgcggatgca gcaaagtatc
    30901 gatccggcga tgcgttcgcc gctgcgctgg tcgatttttg gcttcctgct cctgtctgcc
    30961 acgctgacgc tgatgcggat gcgtaatttg attttgctga tggaaaaacg ccgtccgtgg
    31021 gtgagtgaac tgatactgaa aagaggccgt aaatgacccc tgcatttgct tcctggaatg
    31081 aatttttcgc aatgggcggt tacgcctttt ttgtctggct ggcggtggtg atgaccgtta
    31141 ttccgctggt ggttttggtc gtgcactcgg tgatgcagca tcgcgcaatt ctgcgtggtg
    31201 tggcgcaaca gcgggcgcgt gaggcgcgtt tacgtgctgc gcaacagcag gaggctgcat
    31261 gaatattcgc cgtaaaaacc gcttgtggat tgcctgtgct gttctggcag ggttggcact
    31321 gactatcggt ctggtgctat atgcgctgcg ctcgaatatc gatctctttt atacgccggg
    31381 ggaaattctc tacggcaagc gtgaaactca gcaaatgccg gaagtcggtc agcgtctgcg
    31441 cgttggcggg atggtgatgc cgggtagcgt gcagcgcgat cccaattcgc taaaagtaac
    31501 tttcaccatt tacgacgctg aaggttcagt agatgtctct tacgaaggca ttttgccgga
    31561 tctgttccgt gaagggcagg gcgttgtggt gcagggcgaa ctggaaaaag gcaatcatat
    31621 cctcgcaaaa gaagtgctgg cgaaacacga cgaaaactac acgccgccag aagttgagaa
    31681 agcgatggaa gccaatcacc gtcgcccggc gagtgtttat aaggacccag catcatgatg
    31741 ccggaaattg gtaacgggct gctgtgtctg gcgctaggaa ttgcgctgct gctgtccgtg
    31801 tatccgctat ggggcgtagc gcgcggagat gcgcgcatga tggcgtcttc ccgcttgttt
    31861 gcctggctgc tgtttatgtc tgtggctggc gcatttctgg tactggtcaa tgccttcgtg
    31921 gtcaacgact tcaccgtcac ctatgttgcc agcaactcca atacccagct tccggtgtgg
    31981 tatcgcgtgg cggctacctg gggcgcgcat gaaggctcgc tcctgctgtg ggtgctgctg
    32041 atgagcggct ggacctttgc ggtagcgatt tttagtcagc gtattccgct ggatattgtg
    32101 gcccgcgtac tggcgataat ggggatggtc agcgtcggct ttttgctgtt cattctcttt
    32161 acctctaacc cgttctcacg cacgttgccg aacttcccga ttgaagggcg cgatcttaac
    32221 ccgctgttac aggatccggg gctgatcttc catccgcctc tgctctatat ggggtacgtg
    32281 gggttctcgg tggcgtttgc ttttgccatt gcttctttgt tgtgcgggcg tctggacagc
    32341 acttatgcgc gttttactcg tccgtggacg ctggcggcgt ggatcttctt gacgctcggc
    32401 attgtgctcg gttccgcatg ggcctattac gaactcggct ggggcggctg gtggttctgg
    32461 gatccggtag aaaacgcctc gtttatgccg tggctggtgg ggactgcgct gatgcactca
    32521 ctggcggtca ctgaacaacg cgccagcttc aaagcgtgga cattactgct ggcaatcagt
    32581 gccttctcgt tgtgtctgct ggggactttc ctggtgcgtt ccggcgtgct ggtatcggta
    32641 cacgcgtttg cctctgatcc ggcacgcggt atgtttatcc tcgcctttat ggtactggtg
    32701 attggtggtt cgctgctgct gtttgccgcg cgtggacaca aagttcgctc acgcgtaaac
    32761 aatgcgctgt ggtcgcggga atctctgctg ttagcgaaca acgttttgct ggtcgccgcg
    32821 atgctggtgg tattactggg gacgctgctg ccgctggtgc acaagcaact gggactgggc
    32881 agtatttcga ttggcgaacc gttcttcaac accatgttta cctggctgat ggtgccgttt
    32941 gcgctgctgc ttggtgtcgg tcctctggtg cgctgggggc gcgatcgccc acgtaaaatc
    33001 cgcaatttat tgattatcgc cttcatctca acgctggtgc tgtcgctgct gctgccgtgg
    33061 ctgttcgaaa gcaaagttgt ggcgatgacg gtgctcggcc tggcaatggc ctgctggatt
    33121 gctgtgctgg caattgcgga agctgcgctg cgtatttcac gtggcacgaa aaccaccttc
    33181 agttattggg gaatggtggc ggctcacctg gggctggcag tgacaattgt tggtattgcc
    33241 tttagccaga actatagcgt tgagcgtgat gtgcgcatga agtccggcga tagcgtcgat
    33301 attcatgaat atcgcttcac cttccgtgat gtcaaagagg tgactggccc gaactggcgt
    33361 ggcggtgtgg cgactatcgg cgtaacgcgc gatggcaagc cggaaacggt gctgtatgcg
    33421 gaaaaacgtt attacaacac tgccgggtcg atgatgaccg aagcggcaat tgacggcggc
    33481 atcacgcgtg acctgtacgc cgcgctcggt gaagagctgg aaaacggcgc gtgggccgtg
    33541 cgtctttact acaaaccatt tgttcgctgg atttgggcgg gcgggctgat gatggcgttg
    33601 ggcggactac tgtgtctgtt tgatcctcgc tatcgtaagc gcgtgagtcc gcaaaaaact
    33661 gcgccggagg ccgtatgaag cgcaaagtat tgttaattcc gttgattatc ttcctggcga
    33721 ttgccgcggc gctgctgtgg cagctggcgc gtaatgccga aggggatgat ccgaccaatc
    33781 tggaatcggc gctcattggc aagcctgtgc cgaagtttcg tctcgaatca ctggacaatc
    33841 cggggcagtt ttatcaggcg gatgtgctga ctcagggcaa accagtactg cttaacgtct
    33901 gggcgacctg gtgtccgacc tgccgtgcgg aacatcaata tctgaatcag ctttcagcgc
    33961 agggcattcg cgtggtcggc atgaactata aagacgatcg ccagaaggca atcagctggc
    34021 tgaaagagct gggcaatcct tacgcgctaa gcctgtttga tggcgacggc atgttagggc
    34081 tggatctcgg tgtctatggc gcgccagaaa cgttccttat tgacggcaac ggcatcattc
    34141 gctatcgcca tgcgggcgat ctcaatcctc gcgtctggga agaagagatc aagccgctgt
    34201 gggagaaata cagtaaggag gccgcacaat gaggttttta ttgggcgtgc tgatgctgat
    34261 gatctccggc tcagcgctgg cgaccatcga cgtgttgcag tttaaagatg aagcacagga
    34321 acaacagttc cgtcagctca ctgaagaact gcgctgcccg aaatgccaga ataacagcat
    34381 tgccgattcc aactcgatga ttgccaccga cctgcgccag aaagtgtatg aactgatgca
    34441 ggaaggtaaa agtaagaaag agattgtcga ttatatggtg gcgcgttacg gcaacttcgt
    34501 cacttacgat ccgccgttaa cgccgctgac cgtgctgctg tgggtgctgc cagtagtggc
    34561 tattggcatt ggcggttggg tcatttacgc ccgctcgcgg cgtcgggtac gcgtggtgcc
    34621 ggaagcgttt cctgaacaaa gcgtgccgga aggtaagcgt gccggatatg tcgcttattt
    34681 gccgggcatt gtggtggcgt taattgtggc tggcgtcagc tactaccaga ccggaaatta
    34741 tcaacaggtg aaaatctggc agcaggccac ggcacaggct ccggcgttgc tggacagggc
    34801 gctggatccg aaagccgatc cgctcaacga agaagagatg tcgcgtcttg cgctggggat
    34861 gcgtactcaa ctgcaaaaaa atccgggaga tatagaaggc tggattatgt tgggccgcgt
    34921 tggcatggcg ctgggtaacg ctagtatcgc caccgatgca tacgctactg cgtatcgcct
    34981 cgatccgaaa aacagcgatg ccgcactggg ttatgctgaa gcgttgacac gttcatctga
    35041 tcccaacgac aaccgcctcg gcggcgaact gctgcgccag ttggtgagaa gcgaccacag
    35101 caatatccgt gtgttaagca tgtatgcgtt taatgccttt gagcagcagc gatttggcga
    35161 agccgttgcc gcgtgggaga tgatgttgaa actcttacct gccaacgata ctcgccgtgc
    35221 ggtgattgaa cgtagtatcg cgcaggcgat gcaacatttg tcgccgcagg agagtaaata
    35281 aaaagaggcc cgatggtgat gccatcgggc tatttttatt gtgccccgcg ttgttgcagg
    35341 aacagaatgg tggctgccac gcgtgagcgg acattgagtt tgcgcagcag attgcgaata
    35401 tgtactttta ctgtctgctc tgaaatattc aacaccgagg caatctgttt atttgacagc
    35461 ccctgtgcca gctcgtgcag aacatccagc tcgcgctccg tcagcacgct gaagggatct
    35521 tcttccgcgc caaacatttc acgttcacgt aagtactgat tgacgcgttc gctaaagact
    35581 ttgctgcctt tcgctccagc acgaattgct tccagcaata cttccgggtc gctgtctttc
    35641 aacagataac cgtctgcgcc tgcgtctatc agcgcaaaga catcgctgga ggcatctgat
    35701 acggtcagga taataatttg cgcggtaacg ccatccctgc gcaaggcatt gagggtatcc
    35761 aggccactca tacctttcat attgagatcc agcaagatca cgtcgatatc cagtctattc
    35821 gccagatcga tcgcgctcgc gccgtcgccc gcttcggcga ccacttcaaa gccaggatca
    35881 agctccagta actgacgaac accgcgtcgc ataagtggat gatcatccac aatcatcacc
    35941 tgaaaaggcg ttgcttcagg catagtagtc tcctgaggtt ttattagaat cattatgttt
    36001 gagcgtagcg ttggtaacgc taccaggcgc gtgaatgtta ccccatttta tgtatggaca
    36061 tgtagtgaaa actacaacct tgtcgttcgc tttggcatca ttttcattat aaagacggaa
    36121 ttttgcatca gcttctcatc aagaaaaaca actaaaaaat caatcacaat acttgtgcat
    36181 ttctttaaac agtttttaaa aactgactcc cggtatggag ctatgggtat tttctgtacc
    36241 caatgctttt aacagcaatt aatttcatag gatgaaagct caatgcatca atctggttct
    36301 gtttctcttt gtcgttccgc aatatctgtt ctggtggcta cagcgttata ttcacccata
    36361 gcattggcat caacagttga gtatggtgag acagttgatg gtgttgtcct ggaaaaagat
    36421 atccagctgg tttatgggac cgccaataat acgaaaatca atcctggcgg agaacagcat
    36481 ataaaagaat ttggtgtaag taataatact gaaattaacg gagggtatca gtacattgaa
    36541 atgaatggcg ccgcagaata ctcagtatta aatgacggtt atcaaattgt tcaaatgggt
    36601 ggcgcggcaa accagactac gctcaataat ggtgtgctac aggtttatgg cgcagcgaat
    36661 gataccacga ttaaaggcgg gcgcttaatc gttgaaaaag atgggggggc cgtctttgtc
    36721 gctatcgaaa agggaggact actggaggtt aaagaggggg gattagcgat tgcggtagat
    36781 cagaaagcag gcggtgctat taaaacaacc acgcgggcca tggaggtatt cggaacaaac
    36841 cgtctcggtc agttcgatat caagaatggt attgctaata atatgttgtt ggaaaacggc
    36901 ggaagtttgc gagttgaaga aaatgacttc gcttataata ccactgtaga tagtggcggc
    36961 ttactggagg ttatggatgg cgggactgta actggcgttg ataaaaaagc aggcggaaaa
    37021 ttaattgtct caacgaatgc gctggaagtg agtggtccaa acagtaaagg ccaatttagt
    37081 ataaaagatg gtgtgtcaaa aaattatgaa ctggatgatg gttccgggct cattgttatg
    37141 gaggacacgc aggccattga tactatcctt gatgagcatg ccactatgca atcgctggga
    37201 aaggatactg gtacgaaagt gcaggcaaat gcggtatatg atctcggtcg atcatatcag
    37261 aatggaagta tcacgtattc ctcaaaagcc atctctgaaa atatggttat caacaatggc
    37321 cgcgctaacg tctgggctgg cacaatggtt aacgtttcag tcagagggaa tgatggcatt
    37381 cttgaggtca tgaagccgca aataaattat gcacccgcaa tgttggtggg taaggtagtg
    37441 gtttctgagg gcgcttcttt tagaacgcat agtgccgtgg ataccagcaa agcggacgtt
    37501 tcgctcgaaa atagcgtatg gaccatcatt gccgatatca ctacgacgaa ccaaaacacc
    37561 ctcctcaact tagccaacct tgcgatgtct gacgcaaatg tgattatgat ggatgagcca
    37621 gtgactcgtt catcagtgac ggcaagtgcg gaaaatttca ttacgttgac caccaatacc
    37681 ctgtcgggaa acggcaattt ttatatgcgt accgatatgg ctaatcatca gagcgatcag
    37741 ctcaacgtca ccggtcaggc aacaggtgat ttcaaaatat tcgtgacgga caccggtgcc
    37801 agcccggcag caggagatag ccttacactg gtaacaacgg gcggcggtga tgctgcattt
    37861 acgttgggca atgccggagg cgttgttgat atcggtacgt atgaatatac cttgctggat
    37921 aatggcaacc atagctggag tttggcagag aatcgcgcgc aaattacccc ttcaaccact
    37981 gatgtgctga atatggcggc agcacaaccg ctggtatttg atgcagaact ggacaccgtg
    38041 cgtgagcgtc ttggtagcgt aaaaggcgtt aattacgata cggcgatgtg gagttcggca
    38101 attaacaccc gcaacaacgt gaccactgat gcgggcgctg gctttgagca aacattgacc
    38161 gggctgacgc tcggtatcga tagccgtttc tcccgtgaag aaagcagcac aattcgcggc
    38221 ttgttctttg gttactctca ttctgatatt ggttttgatc gcggcggcaa aggcaatgtc
    38281 gatagctata ccctgggggc ttatgccggt tgggagcatc agaacggtgc ctatgttgat
    38341 ggagtggtga aagttgaccg ttttgccaac accatccatg gcaagatgag taatggggca
    38401 acagcgtttg gcgattacaa tagtaacggc gcgggtgctc atgtcgagag cgggttccgt
    38461 tgggttgacg gattgtggag tgttagaccc tatctggcct ttaccggctt taccacagat
    38521 ggtcaggact acacgttatc aaacggcatg ccgcggatgt gggaaatacc cggatattac
    38581 gcgctgaagc gggaacggcg gtaagctatc acatggacct gcaaaacggt acgacgctgg
    38641 aaccctgact gaaagccgcc gtgcgtcagg aatacgccga ttctaaccag gtgaaagtta
    38701 atgacgatgg caaatttaat aatgatgtgg ctggaacccg tggcgtttat caggctggga
    38761 taaggtcatc gtttaccccg acgttaagcg gtcatttgtc agtcagctat ggcaatggcg
    38821 caggggtaga atcgccgtgg aatactcagg cgggtgtggt ctggacgttc tgataacaaa
    38881 aaataaacag gctgtgatgt gtcacggtct gtttatcgaa ttaattgcag atataaaaaa
    38941 aaccaaccgt aaaggttggt tttttcttgg gatttttgca cgagagtagt ttaattgagt
    39001 atgtaactac atgattttaa ataatatgct tgattcaact tttaaaccgt ataactaaac
    39061 gtataactaa aatcatttgc tgaaaaagtg tacaatttaa atgcgagttt tcctgtggta
    39121 aaagctccaa ccgtaacgat gttcgttatg gttgccttga tggtctgcgt ggtcattatg
    39181 accattcagg atttgaaggg tgacgcttcc gacttcctca aattgaggtt tccgaaaata
    39241 tcagcgggtt agcgattaat ctgaccatta attaaactgc gaaaatttcg taattatccc
    39301 gatatgggga tcaccatatc gggattgatg ccggaaaaat cagcttttcg ccagaacggt
    39361 gacggcctta tcgagttttt gccatccgca ataggtgatt gccctgaacg ctgtagcgtc
    39421 attctggaaa aggctaaccg tccctgattc ggtttttatt accgcctcat cagttggggt
    39481 tataactgtg gcttcaacgt taataatcag cttttgagcg tctatcagca tcatgcgatc
    39541 cgcctgttcg ctcacaatta ccggtattct caaaagctct atctgccaga actcagcatc
    39601 agtaagctgt aaggcgtttt ctacgggcaa gattagcgac gacgtggcaa gcgtgttacc
    39661 gttcgctatg tgctttttga aaagctctat gaagttttcg gcctcatcag caccattaag
    39721 cacgccctca ggggatacgc ccgccacctc ctggtcactg gaaagaaatt ttttatctat
    39781 ttctttcacc gattccgatg tgatcaagtc tctgatcgct gaattggtac aaggggtgct
    39841 aaatttgatt agctcgttat tcagcatcac aatagcgcca agtttgcaca gttttagcgc
    39901 actttcgttt acttctccct ctttgacagg gatttcttcg gcttctttaa gccatttact
    39961 acccttgcct gatattgact gcaattttgc gccaaacgga agattcagcg cgctgctgat
    40021 tagcttgcct ggtacagact gcgaaaaaat atactcgcca agctctctta ctatcggctc
    40081 tgccaccagc gacggcatat tatccgtagt cattccggca acaatggatt tttcaagaaa
    40141 tttcgatact gccggatcat tttttttcgt catttcaatt gcgtctgcat ggctgaattt
    40201 acacgatgta agcacgctgg cggcgcgaac tgccgacacg cctttcatgt ttttagaaat
    40261 catcatcttc ttcctcctgg taacatgggg ttagggtttc gtgaaaatat ggcatcagaa
    40321 cagcggcagc ctgtaaccgt atttcggttg gctgctgctt gtcgttcatg atttccgtga
    40381 gtacatcaat agggtctgtg atgtattcgc tcatagttcg ctacctcagt gttttaagcc
    40441 agttggtggc ttagggatgg ggaaccgatt tgaggagaca ttagcggcat tgagtttttt
    40501 aacctctttt ttcccaatgc tgccgatctt gcggtgggtg aactgggcca gcttaaacgc
    40561 ggcatctaaa gccagttttt gatctgtgtc catgttgtca atcagtatcc tgcccattgc
    40621 tttgatgggg tcgggtaggc cgttatccat catctcaatg gtgttaattg atgcccgttc
    40681 cggtgtaact ttttcggtaa ctttctttgt aacttttttc ggttttgtaa cctgaaaagt
    40741 tacatcagaa gttacagcct taagatactt tttcacctcc ggatcattgg atagctgcca
    40801 gcctttaacc cttgcagtct tttcgctgta tcccgcatgt atcgcggctt ccgttggatt
    40861 tgtgcccttt gataacgcat cagcaaaccg cttttttttg gtcgttaatg ccatgcaaaa
    40921 acctcgtttt gagggggtgt aacttttcgt gtaacttttt tgaaaagcaa aattttctat
    40981 gaatgagacg gggggcggtg tccagggcga tcgactttcc ggccggaaca ctccccccct
    41041 gtcattgctc accgagtaac gagatcaggc cttcaacttc atcactcagc ttgtttattc
    41101 gtgtctcaag ctctgcgatc ttttccatca atggcgctat ggtgtctctt gtttcctcgc
    41161 caatggcttt aatgatgctt gcctcgcgtt ctgtgatagc cattgtttac gcctcacttg
    41221 ttgcaatgcc caggcgttcg cgtaacagta cgcgcgcaag atgccctatt gttggtgttg
    41281 ccgccctgcc gtagatacct gattcattca tccggcgcac aatatcgaac tgcaactttt
    41341 taagctcgtt aaggatttca tgatcaatca tcacggtagc gctttgcttt atctcttcca
    41401 ctttgttttc tcttgatact tatcattgca taaccagtat tttcgataga ttatcattaa
    41461 ttaaccatct ttgaagatat atgagatcga aaaaaagccg gattgctccg gctatgtgct
    41521 cacttcttcg gttattctcc agcgctttta acgttgatgg ttgttacctg ttccgcttcg
    41581 gcaatctccc gttctgtcag cgtggcaaag tttgccgccg ctgtggtcat gaatgcgctt
    41641 atcagttcgg gatgctcctt cgcgtatcct tcccccgcgt ggcggtctat cgttctgatt
    41701 gccaccttta aggaaagctc tgtcatgtct aacgctttat attttggcgc tgtcttatct
    41761 ctggtttttc tgttcattcc ccactactcc ccacttttgc tccccacttt ttgaaattac
    41821 ccaccgtctc cccacctctt ttttttgtga ttctaacgtt atgtttttca atatgttttt
    41881 tactccccac ttttttgggt atatacaggt gggaaagtgg gtaattatgt ttcaattttg
    41941 ttaaattccc cactcccccc acttttactc cccacttttt acagcgggcg cacgtcatca
    42001 ccgtcaataa cgattacgcc atcattttcc agcttgtaca gccagcgccg gaagtgcttc
    42061 atctcatacc ccagcttttt catatcgtcg cggagaaggg cgatagtgca cgcctccccg
    42121 tgtgctgttc gtgttctgat gcactgccat agcgcggcgt gattctccgt cttgttccct
    42181 gcctcctcga tgcgctccag ttcaacggga gggcgcggct tatccaccac caccagcgac
    42241 gtgattaact ccccgtcagc gtcggtaaaa agctccacca cgcgtaagtc atatgctgct
    42301 tctttgagtt cctccgcgtc cttcattttg gtacatgaga taaccagcgc ttcgctgcct
    42361 gcgtcctccc tgcgtatccg gtattcggcg tccagtgatg cacgaaatgc actggaacca
    42421 cgcgccccct ttgtttcatc cttgccggaa tggtgaacca ccagcaccgt tgcccctgtg
    42481 cgccgtttca gttcgtcaca cccacggata aacgccccca tatcacggga atcattttca
    42541 tcattgccgc caaagcagcg cgccagcgta tccagaataa tcattcgaac cggtttaccc
    42601 gtttcccgtt ccacctgacg ggcagcgata accatttcct caacatcaag cggggcagcc
    42661 ggaaagatgg gacggtttac cagatacaga tttttcacct gctcaccgtg cacaacctcc
    42721 caggctttta cacgacgcgg aacgccgata ccgccttcac caaccacata gagaacagcg
    42781 ccatgcgcca ccctgcggcc tccccactgg cggcccgtgg aaacgtgaca cgcccacgaa
    42841 cccgcaagga acgatttata tgaaccgctc gccccgtata tgctgcacag cgataccgcc
    42901 ggaataatcc ccttaaccac gtaatcaagt tgtgtgtcgt atccggcaga tcccacgctc
    42961 attggtagcg tggtttttcg ctggtggtgt ttttcttccc ctggcttttt cccccgctcc
    43021 cgttccagat attcgcgcca gttctcccgc ttctggctgt gcatcccttc ggggtaataa
    43081 ttcgcatcag ttacacccgc cgccgccagc ttgtgcgcaa tggcattaat atttgatggc
    43141 ctgatgtggc ctgccttgta cagccggaca caatagcgcc cctcgtcgat gattctcagg
    43201 tctgccagtt catccagttg atcatcagcc agcacaacgg gaggcacatt atcgccagcc
    43261 agtcgcccgt cctgttcctg ccactgtttc gcatgtgccc acgcatcact acccgcaaaa
    43321 atgatgactt cggtcatctt gtcgtaaggc tgttttttta agttcggtgc gcttttcatt
    43381 tcttgcccct gaatccgtta atcatggttt tcagcttctg gatgtttgcc cgtgccctgg
    43441 cgttgctggt gggcacgtta tgcggcgcgg tctgtaccag agaaaaatca cgccggaact
    43501 gataaacagg catcacgcaa tcatattcgt aaccttcacg gcggtaagtt acgcaccgtc
    43561 ccgccacgcc cttaatcatt accgtgccgc cgtactggtc gcggtaaata tcaccgcgcg
    43621 taaatttagg gtgagtgttg ccactggcag ttaagccaga atatttaagt ttcattattt
    43681 ttattctccg gtgtgctgtt cgttatatct gtcgtgcaat gtctctattt cctgcaactc
    43741 atttattaca gactcaagaa gcgttattaa tgatgtaata atccgcgttt tttttgcatt
    43801 acgttcatta tcgccaaagg attcaaggca catccgcaat atttccatca tgttttcact
    43861 gtgagaaagt gcaagtgatg cgcggtctat tgtttcaaga taaatatcac gcatggctta
    43921 catcctcagg aaattttctt ctgtaacgcg cctctgccac atattccgca taatcggcgg
    43981 cgatattcag tacatcaagt cccgttgatt tatattctct cgtggaaagt aagaaaaaag
    44041 ccgctctaat aagttctggc attgacgaaa gcgcatcagc cgcatcatca ggaacgccgg
    44101 aaaattcttg tttcagggaa ttaaaacgat catcacgcat aacccccccc ccattttcac
    44161 aatcagcaac aagaatattt ttagcgtcat tcagcgaccg cgttgcgatg tatcggatac
    44221 attccagggc gaactgtgtc cggtctttct gttcttctcc ctgcgcaagc tctgccgtgc
    44281 attcaatatc aataagtgcg tgtatcagcg tagtgagcgc ggcggctgcg tccggtgtgg
    44341 ttttattgca catgtacccc tccgcatttt tttcgttaga aataagcgtt cttctttcct
    44401 gttcatcgat caggaatacg cagacctcac cgctaaggcg tttaagtaag ctgatgattg
    44461 cccctgattc tctgtcggtc atcatgcttg ggtaatcctc tgccagtgcg caaataactt
    44521 cgatttggtg ggcgcgttct gctgcctgtt gtagtgtgat ttcctggctc ataagcctac
    44581 cccctgacga atacgggcag cgaataccat cacgcagcca gccggggatt gcaggcgtgc
    44641 ttcctgttcg ctggtggcct caat
//
LOCUS       DAZGTR010000024        44220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGTR010000024 DAZGTR010000000
VERSION     DAZGTR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.024"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(45..1409)
                     /gene="mnmE"
                     /locus_tag="ACHLCY_001511"
     CDS             complement(45..1409)
                     /gene="mnmE"
                     /locus_tag="ACHLCY_001511"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP0368804.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDANGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLYDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1515..3161)
                     /gene="yidC"
                     /locus_tag="ACHLCY_001512"
     CDS             complement(1515..3161)
                     /gene="yidC"
                     /locus_tag="ACHLCY_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP0368805.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQHISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3164..3421)
                     /gene="yidD"
                     /locus_tag="ACHLCY_001513"
     CDS             complement(3164..3421)
                     /gene="yidD"
                     /locus_tag="ACHLCY_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP0368806.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3385..3744)
                     /gene="rnpA"
                     /locus_tag="ACHLCY_001514"
     CDS             complement(3385..3744)
                     /gene="rnpA"
                     /locus_tag="ACHLCY_001514"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP0368807.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3761..3901)
                     /gene="rpmH"
                     /locus_tag="ACHLCY_001515"
     CDS             complement(3761..3901)
                     /gene="rpmH"
                     /locus_tag="ACHLCY_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP0368808.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4131..4211)
                     /locus_tag="ACHLCY_001516"
     CDS             complement(4131..4211)
                     /locus_tag="ACHLCY_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368809.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4508..5911
                     /gene="dnaA"
                     /locus_tag="ACHLCY_001517"
     CDS             4508..5911
                     /gene="dnaA"
                     /locus_tag="ACHLCY_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP0368810.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNIPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNTLLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILIKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5916..7016
                     /gene="dnaN"
                     /locus_tag="ACHLCY_001518"
     CDS             5916..7016
                     /gene="dnaN"
                     /locus_tag="ACHLCY_001518"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP0368811.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7016..8089
                     /gene="recF"
                     /locus_tag="ACHLCY_001519"
     CDS             7016..8089
                     /gene="recF"
                     /locus_tag="ACHLCY_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP0368812.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8118..10532
                     /gene="gyrB"
                     /locus_tag="ACHLCY_001520"
     CDS             8118..10532
                     /gene="gyrB"
                     /locus_tag="ACHLCY_001520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP0368813.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10773..11171
                     /gene="yidB"
                     /locus_tag="ACHLCY_001521"
     CDS             10773..11171
                     /gene="yidB"
                     /locus_tag="ACHLCY_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP0368814.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11286..12098
                     /gene="yidA"
                     /locus_tag="ACHLCY_001522"
     CDS             11286..12098
                     /gene="yidA"
                     /locus_tag="ACHLCY_001522"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP0368815.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12144..12800)
                     /gene="yidX"
                     /locus_tag="ACHLCY_001523"
     CDS             complement(12144..12800)
                     /gene="yidX"
                     /locus_tag="ACHLCY_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP0368816.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYHWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13078..13767
                     /gene="dgoR"
                     /locus_tag="ACHLCY_001524"
     CDS             13078..13767
                     /gene="dgoR"
                     /locus_tag="ACHLCY_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP0368817.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13764..14642
                     /gene="dgoK"
                     /locus_tag="ACHLCY_001525"
     CDS             13764..14642
                     /gene="dgoK"
                     /locus_tag="ACHLCY_001525"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP0368818.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14626..15243
                     /gene="dgoA"
                     /locus_tag="ACHLCY_001526"
     CDS             14626..15243
                     /gene="dgoA"
                     /locus_tag="ACHLCY_001526"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP0368819.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15240..16388
                     /gene="dgoD"
                     /locus_tag="ACHLCY_001527"
     CDS             15240..16388
                     /gene="dgoD"
                     /locus_tag="ACHLCY_001527"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP0368820.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16463..17800
                     /gene="dgoT"
                     /locus_tag="ACHLCY_001528"
     CDS             16463..17800
                     /gene="dgoT"
                     /locus_tag="ACHLCY_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0368821.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17797..18861)
                     /gene="cbrA"
                     /locus_tag="ACHLCY_001529"
     CDS             complement(17797..18861)
                     /gene="cbrA"
                     /locus_tag="ACHLCY_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP0368822.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCSGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKRIMRSGMAHIPQLKD"
     gene            18962..20176
                     /gene="yidR"
                     /locus_tag="ACHLCY_001530"
     CDS             18962..20176
                     /gene="yidR"
                     /locus_tag="ACHLCY_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP0368823.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYMPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAEVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20178..20510)
                     /gene="yidQ"
                     /locus_tag="ACHLCY_001531"
     CDS             complement(20178..20510)
                     /gene="yidQ"
                     /locus_tag="ACHLCY_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0368824.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20750..20822
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20816..21229
                     /gene="ibpA"
                     /locus_tag="ACHLCY_001532"
     CDS             20816..21229
                     /gene="ibpA"
                     /locus_tag="ACHLCY_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP0368825.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21341..21769
                     /gene="ibpB"
                     /locus_tag="ACHLCY_001533"
     CDS             21341..21769
                     /gene="ibpB"
                     /locus_tag="ACHLCY_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP0368826.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21966..23627
                     /gene="yidE"
                     /locus_tag="ACHLCY_001534"
     CDS             21966..23627
                     /gene="yidE"
                     /locus_tag="ACHLCY_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP0368827.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23624..24340)
                     /gene="yidP"
                     /locus_tag="ACHLCY_001535"
     CDS             complement(23624..24340)
                     /gene="yidP"
                     /locus_tag="ACHLCY_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0368828.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24636..26252
                     /locus_tag="ACHLCY_001536"
     CDS             24636..26252
                     /locus_tag="ACHLCY_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0368829.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLDIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26252..27066
                     /locus_tag="ACHLCY_001537"
                     /pseudo
     CDS             26252..27066
                     /locus_tag="ACHLCY_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27063..27956)
                     /gene="yidL"
                     /locus_tag="ACHLCY_001538"
     CDS             complement(27063..27956)
                     /gene="yidL"
                     /locus_tag="ACHLCY_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0368830.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRNKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28123..29838
                     /gene="yidK"
                     /locus_tag="ACHLCY_001539"
     CDS             28123..29838
                     /gene="yidK"
                     /locus_tag="ACHLCY_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP0368831.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29835..31328
                     /gene="yidJ"
                     /locus_tag="ACHLCY_001540"
     CDS             29835..31328
                     /gene="yidJ"
                     /locus_tag="ACHLCY_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0368832.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31375..31824)
                     /gene="yidI"
                     /locus_tag="ACHLCY_001541"
     CDS             complement(31375..31824)
                     /gene="yidI"
                     /locus_tag="ACHLCY_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP0368833.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31934..32281
                     /gene="yidH"
                     /locus_tag="ACHLCY_001542"
     CDS             31934..32281
                     /gene="yidH"
                     /locus_tag="ACHLCY_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP0368834.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32271..32633
                     /gene="yidG"
                     /locus_tag="ACHLCY_001543"
     CDS             32271..32633
                     /gene="yidG"
                     /locus_tag="ACHLCY_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP0368835.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32630..33127
                     /gene="yidF"
                     /locus_tag="ACHLCY_001544"
     CDS             32630..33127
                     /gene="yidF"
                     /locus_tag="ACHLCY_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP0368836.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33135..34319)
                     /gene="emrD"
                     /locus_tag="ACHLCY_001545"
     CDS             complement(33135..34319)
                     /gene="emrD"
                     /locus_tag="ACHLCY_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP0368837.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            34401..34475
                     /gene="ysdE"
                     /locus_tag="ACHLCY_001546"
     CDS             34401..34475
                     /gene="ysdE"
                     /locus_tag="ACHLCY_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP0368838.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34738..34827)
                     /gene="tisB"
                     /locus_tag="ACHLCY_001547"
     CDS             complement(34738..34827)
                     /gene="tisB"
                     /locus_tag="ACHLCY_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP0368839.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35392..35490
                     /gene="ivbL"
                     /locus_tag="ACHLCY_001548"
     CDS             35392..35490
                     /gene="ivbL"
                     /locus_tag="ACHLCY_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP0368840.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35596..37284
                     /gene="ilvB"
                     /locus_tag="ACHLCY_001549"
     CDS             35596..37284
                     /gene="ilvB"
                     /locus_tag="ACHLCY_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP0368841.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37288..37578
                     /gene="ilvN"
                     /locus_tag="ACHLCY_001550"
     CDS             37288..37578
                     /gene="ilvN"
                     /locus_tag="ACHLCY_001550"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0368842.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37653..38243
                     /gene="uhpA"
                     /locus_tag="ACHLCY_001551"
     CDS             37653..38243
                     /gene="uhpA"
                     /locus_tag="ACHLCY_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP0368843.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38243..39745
                     /gene="uhpB"
                     /locus_tag="ACHLCY_001552"
     CDS             38243..39745
                     /gene="uhpB"
                     /locus_tag="ACHLCY_001552"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP0368844.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39755..41074
                     /gene="uhpC"
                     /locus_tag="ACHLCY_001553"
     CDS             39755..41074
                     /gene="uhpC"
                     /locus_tag="ACHLCY_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0368845.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGVSALLLLPFLNAQAPREA"
     gene            41330..42721
                     /gene="uhpT"
                     /locus_tag="ACHLCY_001554"
     CDS             41330..42721
                     /gene="uhpT"
                     /locus_tag="ACHLCY_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP0368846.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42766..44220)
                     /gene="adeD"
                     /locus_tag="ACHLCY_001555"
                     /pseudo
     CDS             complement(42766..44220)
                     /gene="adeD"
                     /locus_tag="ACHLCY_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase"
BASE COUNT        10174 a        11370 c        12016 g        10660 t
ORIGIN      
        1 gcgaagccgc atctgacagt cagaatacgg cttcgtaagc gcggttactt accaatacag
       61 aagctggaga aaatccgccc cagcaggtcg tctgaagtaa attccccggt gatttcgctt
      121 aagttctgct gtgccaggcg caactcttcc gccagcagtt cacccgccca ggcacctaac
      181 agttgcgctt taccttgctg caaatgctcc gctgcctgtt ccagcgcctg taagtggcga
      241 cggcgcgcca ggaaaccgcc ttccatgttg gtgtcaaagc ccatgctctg tttgagatgg
      301 ttacgcagca cgtccacgcc ttcaccagtc cttgccgaga gacgaattaa cgcgtgaccg
      361 ttcacttcac tcattcccag cgtttcgccg gtgatatcgg ctttattgcg caccacggtg
      421 atcggcagtt tcgctggcag acgggcgata aattccggcc agatctctgc cggatccacg
      481 gcgtctgttg tggtgccatc gaccataaac agcacccggt cggcctgttc aatttcctgc
      541 cacgcgcgct cgatcccaat acgttccact tcgtcactgg cttcacgtag cccggcggtg
      601 tcgatgatat gcagcggcat tccgtcaatg tggatatgct cacgcaacac gtcacgcgta
      661 gttccggcga tatcggttac gattgctgct tcacggccag ccagcgcgtt taacaggctc
      721 gatttaccgg cgttaggacg tccggcaatc accactttca tcccttcgcg caacaaacta
      781 ccctgacgtg cttcagcacg cactgcatca agatcggcaa taacgtcatt gagctgggct
      841 tcaattttgc cgtcggagag gaaatcgatc tcttcatcag ggaaatcaat tgccgcttcg
      901 acgtaaatgc gcaagtgggt gagggcttct accagatgat taacccgtgc ggagaatgcg
      961 ccttgcagcg agttaagtgc cgaacgggcc gcctgttccg agctggcgtc gataagatcg
     1021 gcaatcgcct cagcctgggc taagtcgagt ttatcgtaaa gaaacgcgcg ttcggaaaac
     1081 tcaccagggc gagcaatccg caggccggga atggtcagaa tgcgttttaa cagcaggtcg
     1141 aggatcaccg gaccgccatg accctgcagt tcaagcacat cttcgccggt gaacgagttc
     1201 gggccaggga accatagcgc aatcccctga tcgagcacgc tgccgttggc gtctttaaat
     1261 ggcaaataat cggcgtagcg cggcttaggt agtttaccca gcacggtttc ggcaacttca
     1321 cgggctttga ggccggagat gcgcaggatg ccaacaccgc cacgtcccgg aggcgtggcc
     1381 tgggctacga tagtgtcatt atcgctcatg atgttcctgt tgctttgtgt ggcggatgcg
     1441 cggtgcttat ccgccctacg aaaagaaaaa aggcggtcaa ctgaccgccc ttattttagc
     1501 gaaaactcac cgaatcagga ttttttcttc tcgcggctat gcaggccacg tttttccaga
     1561 ccacggtaaa tcagctgctg ctgaataatg gttaccaggt tgctgacgat atagtacagc
     1621 accagacctg acgggaacca caggaagaac acggtgaaga tgaccggcat aaaggtcatg
     1681 atcttctgct gcatcgggtc ggtcactgtg gtcggcgaca tcttctgaat gaagaacatc
     1741 gttacgccca tcaggatcgg caggatgtag tacgggtcct gtgccgacag gtcgtggatc
     1801 cacagtgcaa acggtgcctg acgcagttca acggaaccca tcagcatgta gtacaacgcc
     1861 aggaagattg gcatctggat cagcagcggg aagcagccgc ccagcgggtt aaccttctca
     1921 gctttgtaca gcgccatcat ttcctggctg atatgctgtt tgtcatcgcc cagacgctca
     1981 cgcattgcct gaatcttcgg ctgcagcata cgcatcttcg ccatggaggt gtactgcgct
     2041 ttggtcagcg ggtacatgat gccacgaacg ataaaggtga tgatgataat ggagaagccc
     2101 cagttaccca caaagctatg gatccatttc agcagtttga acagcggctg agagatgaac
     2161 cacaaccaac cgtaatcaac ggtcagatcc aggtgcggag caacagctgc cattttgtcc
     2221 tggatttccg ggccaaccca cagggtgctg ttcatcgcgc cagtctgacc aggctgaacc
     2281 agtaccggct gagatttata gccgatagcg gcgatgccgt tacccagatt agcggtatag
     2341 aagttgttgg taccgtcgtt atgcgggatc cacgccgtcg cgaaatactg ttgcagcatc
     2401 gccacccaac cacctttcga agagatgttc aggttttcgt tatcggcaat ggtatcgaac
     2461 ttgtatttct catacttctc gtcaggcgtg gagtacgccg cgccacggaa ggtgtgcagt
     2521 gcgaagttgc tgcttccggt atcgagatgc ggtggcagag tgatggattg cttcaactga
     2581 ccaaaggtgg agatttccag cggtttctcg ccagcgttct gcacgttgta gttgacgttg
     2641 acagcgtaat caccacgttt caggacaaac gttttggtaa acgtgttgcc tgccgcgtcg
     2701 gtatacgtca tcggcacctg cagttcgttt tgaccttcag ccagcacata agcgtctttt
     2761 tcaacgttat acagcggacg cgggccgtta gccgggttat ccgggccatc acgaccggtc
     2821 agaccgctct gtgcctgata aataaactgc ggtgaagttt ccagcagctg gaacggctgg
     2881 gtagagttca gctctttcgg gtaagcaggc agcagagctt gctcaacatc accaccacgg
     2941 gtgttgatgg tcagatcaag cacgtcggtc ttaaccgaga tcagtttccc ctggccactg
     3001 gccggtacgc cctggtcggc ggcgctaccc gctgcggtgg tcgttgtctg cgtggtctgt
     3061 tgggcctgag gttgcgggtt tttatcctgc tcccaggctt gccagatcat gaaagacacg
     3121 aacagcaaag cgatgactaa aagattgcgt tgcgaatcca tcgttagtgt tctctggtat
     3181 caaatggtcc gggcgggacg ggatcgtcac caccagggtg taaagggtgg cattttaata
     3241 cgcgtttcac cgtcaaccaa ctgcctttta tcactccaaa cctgcgcaat gcctcaattc
     3301 cgtagcttga acaggttgga gtgaaacgac aatgcggccc gagtagcgga ctaatcaggc
     3361 gttgatagac ccgaatgagg gctatcagga cccgcgagcc aggcgacagt ggcggcgcca
     3421 taatttttcc aacgcttccg agagagcacg gttatcgagg tcggcaaccc cttttttcgc
     3481 caccaccacg aaatccatag ccgggagttc atgttggcgc agacggaagc tttcacgcgt
     3541 cagacgttta atccgattgc gttcatgggc gcgtcgaacg tttttcttgg cgactgtaag
     3601 accgatacgg ggatgcccca gcgaattcag gcggccgaga atggtaattt gcggcgtgcc
     3661 agcccgttgt ggctgctgga agacgaatgt gaattgactg ggagttaaca agcgtaactc
     3721 cctgggaaat gcgagcttaa ccactcaggg gttagcttta ttacttagaa acggtcagac
     3781 gagcgcggcc tttagcacga cgacgtgcca gaacctgacg accattttta gtagccatac
     3841 gagcacggaa gccgtgagaa cggttgcgct tcagtacaga cggttgaaaa gtgcgtttca
     3901 tggcgatttc tacctaaact tgaataaatt caatggcttt attggatatc cgccgaaaaa
     3961 tgaaacgatg gacaccgaag ccatgggtga ttaaagaggc cggattgtaa taattgtaca
     4021 ctccggagtc aattctcttt ccttatttac cgcgcttttc cgcacctttt cgcagggaaa
     4081 atgtacgacc tcacaccagt gaaaaccagc atggcgcgcc gggtggagga ttatacgggc
     4141 tgatgggtaa agcgcaagga tcgtcctgga tctttattag atcgattaag ccaatttttg
     4201 tctatggtca ttaaattttc caatatgcgg cgtaaatcgt gcccgcctcg cggcaggatc
     4261 gtttacactt agcgaggtct ggaaagtcct gtggataaat cgggaaaatc tgtgagaaac
     4321 agaagatctc ttgcgcagtt taggctatga tccgcggtcc cgatcgtttt gcaggatctt
     4381 gatcgggcat ataaccgcag acagcggttc gtgcgtcacc ctcaagcagg gtcttttcga
     4441 cgtacgtcaa caatcatgaa tgtttcagcc ttagtcatta tcgacttttg ttcgagtgga
     4501 gtccgccgtg tcactttcgc tttggcagca gtgtcttgcc cgattgcagg atgagttacc
     4561 agccacagaa ttcagtatgt ggatacgccc attgcaggcg gaactgagcg ataacacgct
     4621 ggccctgtac gcgccaaacc gttttgtcct cgattgggta cgggacaagt accttaataa
     4681 tatcaatgga ctgctaacca gtttctgcgg agcggatgcc ccacagctgc gttttgaagt
     4741 cggcaccaaa ccggtgacgc aaacgccaca agcggcagtg acgagcaacg tcgcggcccc
     4801 tgcacaggtg gcgcaaacgc agccgcaacg tgctgcgcct tctacgcgct caggttggga
     4861 taacatcccg gccccggcag aaccgaccta tcgttctaac gtaaacgtca aacacacgtt
     4921 tgataacttc gttgaaggta aatctaacca actggcgcgc gcggcggctc gccaggtggc
     4981 ggataaccct ggcggtgcct ataacccgtt gttcctttat ggcggcacgg gtctgggtaa
     5041 aactcacctg ctgcatgcgg tgggtaacgg cattatggcg cgcaagccga atgccaaagt
     5101 ggtttatatg cactccgagc gctttgttca ggacatggtt aaagccctgc aaaacaacgc
     5161 gatcgaagag tttaaacgct actaccgttc cgtagatgca ctgctgatcg acgatattca
     5221 gttttttgct aataaagaac gatctcagga agagtttttc cataccttca acaccctgct
     5281 ggaaggtaat caacagatca ttctcacctc ggatcgctat ccgaaagaga tcaacggcgt
     5341 tgaggatcgt ttgaaatccc gcttcggttg gggactgact gtggcaatcg aaccgccaga
     5401 gctggaaacc cgtgtggcga tcctgattaa aaaggccgac gaaaacgaca ttcgtttgcc
     5461 gggcgaagtg gcgttcttta tcgccaagcg tctacgatct aacgtacgtg agctggaagg
     5521 ggcgctgaac cgcgtcattg ccaatgccaa ctttaccgga cgggcgatca ccatcgactt
     5581 cgtgcgtgag gcgctgcgcg acttgctggc attgcaggaa aaactggtca ccatcgacaa
     5641 tattcagaag acggtggcgg agtactacaa gatcaaagtc gcggatctcc tttccaagcg
     5701 tcgatcccgc tcggtggcgc gtccgcgcca gatggcgatg gcgctggcga aagagctgac
     5761 taaccacagt ctgccggaga ttggcgatgc gtttggtggt cgtgaccaca cgacggtgct
     5821 tcatgcctgc cgtaagatcg agcagttgcg tgaagagagc cacgatatca aagaagattt
     5881 ttcaaattta atcagaacat tgtcatcgta aacctatgaa atttaccgta gaacgtgagc
     5941 atttattaaa accgctacaa caggtgagcg gtccgttagg tggtcgtcct acgctaccga
     6001 ttctcggtaa tctgctgtta caggttgctg acggtacgtt gtcgctgacc ggtactgatc
     6061 tcgagatgga aatggtggca cgtgttgcgc tggttcagcc acacgagcca ggagcgacga
     6121 ccgttccggc gcgcaaattc tttgatatct gccgtggtct gcctgaaggc gcggaaattg
     6181 ccgtgcagct ggaaggtgaa cggatgctgg tacgctccgg gcgtagccgt ttttcgctgt
     6241 ctaccctgcc agcggcggat ttcccgaacc tcgatgactg gcagagtgaa gtcgaattta
     6301 ccctgccgca ggcaacgatg aagcgtctga ttgaagcgac ccagttttcg atggcgcatc
     6361 aggacgttcg ctattactta aatggtatgc tgtttgaaac cgaaggtgaa gaactgcgca
     6421 ccgtggcaac cgacggccac cgtctggcgg tctgttcaat gccaattggt caatctttgc
     6481 caagccattc ggtgatcgta ccgcgtaaag gcgtgattga actgatgcgt atgctcgacg
     6541 gcggcgacaa tccgctgcgc gtgcagattg gcagcaacaa tattcgcgcc cacgttggcg
     6601 actttatctt cacctccaaa ctggtggatg gtcgcttccc ggattaccgc cgcgttctgc
     6661 cgaagaatcc ggacaaacat ctggaagctg gctgcgatct gctcaagcag gcgtttgccc
     6721 gtgcggcaat tctctctaac gagaaattcc gcggcgtgcg cctgtatgtc agcgaaaacc
     6781 agctgaaaat caccgccaac aacccggaac aggaagaagc ggaagagatc ctcgacgtta
     6841 cctatagcgg tgcggagatg gaaatcggct tcaacgtcag ctatgtgctg gatgttctga
     6901 acgcgctgaa atgcgaaaac gtccgcatga tgctgaccga ttcggtttcc agcgtgcaga
     6961 ttgaagatgc cgcatcacag tcggctgcct atgttgtcat gccaatgaga ctgtaatgtc
     7021 cctcacccgc ttgttgatcc gcgatttccg caacattgaa accgcggatc tcgctttatc
     7081 tcccggcttt aactttctgg taggtgccaa cggcagtggc aaaaccagcg tgctggaagc
     7141 catctatacg ctcggccatg gtcgggcgtt tcgcagtttg cagattggtc gcgtcattcg
     7201 ccatgagcag gaggcatttg ttctccatgg gcgattacag ggcgaagagc gcgagacggc
     7261 gattggctta accaaggaca aacagggcga cagcaaagtc cgcatcgacg gtactgacgg
     7321 gcataaagtc gcggaactgg cgcacctgat gccaatgcag ctgataacgc cagaagggtt
     7381 tactttactc aacggcggcc ccaaatacag aagagcattc ctcgactggg gatgctttca
     7441 caacgaaccc ggatttttca ccgcctggag caatctcaag cgattgctca agcagcgcaa
     7501 tgcggcgctg cgccaggtga cacgttacga acagctacgc ccgtgggata aagaactgat
     7561 cccgctggcg gagcaaatca gcacctggcg cgcggagtat agcgccggta tcgcggccga
     7621 tatggccgat acctgtaagc aatttctccc tgagttttct ctgactttct ctttccagcg
     7681 cggctgggag aaagagacag aatatgctga ggtgctggaa cgtaattttg aacgcgatcg
     7741 ccagctaacc tacaccgcgc atggcccgca taaagcggac ttacgcattc gcgccgacgg
     7801 tgcgccggtg gaagatacct tatcgcgtgg gcagcttaag ctgttgatgt gcgccttacg
     7861 tctggcgcaa ggagagttcc tcacccgtga aagcgggcgg cggtgtctct acctgataga
     7921 tgattttgcc tctgagcttg atgatgagcg tcgtgggttg cttgccagcc gcttaaaagc
     7981 gacgcaatca caggtctttg tcagcgcgat cagtgctgaa cacgttatag acatgtcgga
     8041 cgaaaattcg aagatgttta ccgtggaaaa gggtaaaata acggattaac ccaagtataa
     8101 atgagcgaga aacgttgatg tcgaattctt atgactcctc cagtatcaaa gtcctgaaag
     8161 ggctggatgc ggtgcgtaag cgcccgggta tgtatatcgg cgacacggat gacggcaccg
     8221 gtctgcacca catggtattc gaggtggtag ataacgctat cgacgaagcg ctcgcgggtc
     8281 actgtaaaga aattatcgtc accattcacg ccgacaactc tgtctctgta caggatgacg
     8341 ggcgcggcat tccgaccggt attcacccgg aagagggcgt atcggcggcg gaagtgatca
     8401 tgaccgttct gcacgcaggc ggtaaattcg acgataactc ctataaagtg tccggcggtc
     8461 tgcacggcgt tggtgtttcg gtagtaaacg ccctgtcgca aaaactggag ctggttatcc
     8521 agcgcgaggg taaaattcac cgtcagatct acgaacacgg tgtaccgcag gccccgctgg
     8581 cggttaccgg cgagactgaa aaaaccggca ccatggtgcg tttctggcct agcctcgaaa
     8641 ctttcaccaa tgtgaccgag ttcgaatatg aaattctggc gaaacgtctg cgtgagttgt
     8701 cgttcctcaa ctccggcgtt tccattcgtc tgcgcgacaa gcgcgacggc aaagaagacc
     8761 acttccacta tgaaggcggc atcaaggcgt tcgttgaata tctgaacaag aacaaaacgc
     8821 cgatccaccc gaatatcttc tacttctcca ctgaaaaaga cggtattggc gtcgaagtgg
     8881 cgttgcagtg gaacgatggc ttccaggaaa acatctactg ctttaccaac aacattccgc
     8941 agcgtgacgg cggtactcac ctggcaggct tccgtgcggc gatgacccgt accctgaacg
     9001 cctacatgga caaagaaggc tacagcaaaa aagccaaagt tagcgccacc ggtgacgatg
     9061 cgcgtgaagg cctgattgcg gtcgtttccg tgaaagtgcc ggacccgaaa ttctcctccc
     9121 agaccaaaga caaactggtt tcttctgagg tgaaatcagc ggttgaacag cagatgaacg
     9181 aactgctggc agaatacctg ctggaaaacc caaccgacgc gaaaatcgtg gttggcaaaa
     9241 ttatcgatgc tgcccgtgcc cgtgaagcgg cgcgtcgcgc gcgtgaaatg acccgccgta
     9301 aaggtgcgct cgacttagcg ggcctgccgg gcaaactggc agactgccag gaacgcgatc
     9361 cggcgctttc cgaactgtac ctggtggaag gggactccgc gggcggctct gcgaagcagg
     9421 ggcgtaaccg caagaaccag gcgattctgc cgctgaaggg taaaatcctc aacgtcgaga
     9481 aagcgcgctt cgataagatg ctctcttctc aggaagtggc gacgcttatc accgcgcttg
     9541 gctgtggtat cggtcgtgac gagtacaacc cggacaaact gcgttatcac agcatcatca
     9601 tcatgaccga tgcggacgtc gacggctcgc acattcgtac gctgctgttg accttcttct
     9661 atcgtcagat gccggaaatc gttgaacgcg gtcacgtcta catcgctcag ccgccgctgt
     9721 acaaagtgaa gaaaggcaag caggaacagt acattaaaga cgacgaagcg atggatcagt
     9781 accagatctc tatcgcgctg gatggcgcaa cgctgcacac caacgccagc gcaccggcat
     9841 tggctggcga agcgttagag aaattggtgt ctgagtacaa cgcgacgcag aaaatgatca
     9901 accgcatgga gcgtcgttat ccgaaagcaa tgctgaaaga gcttatctat cagccgacgc
     9961 tgacggaagc cgacctctct gatgagcaga ccgttacccg ctgggtgaac gcgctggtca
    10021 gcgaactgaa cgacaaagaa cagcacggca gccagtggaa gtttgatgtc cacaccaatg
    10081 ccgaacaaaa cctgttcgag ccgattgttc gcgtgcgtac ccacggtgtg gatactgact
    10141 atccgctgga tcacgagttt atcaccggtg gcgaatatcg tcgtatctgc acgctgggtg
    10201 agaaactgcg tggcttgctg gaagaagatg cgtttatcga acgtggcgag cgtcgtcagc
    10261 cggtagccag cttcgagcag gcgctggact ggctggtgaa agagtcccgt cgcggcctct
    10321 ccatccagcg ttataaaggt ctgggcgaga tgaacccgga acagctgtgg gaaaccacca
    10381 tggacccgga aagccgtcgt atgctgcgcg ttaccgttaa ggatgcgatt gccgctgacc
    10441 agttgttcac cacgctgatg ggcgacgccg ttgaaccgcg ccgtgcgttt attgaagaga
    10501 acgccctgaa agcggcgaat atcgatattt aatggcgcta accatgcgag cgtgcctgat
    10561 gcgctacgct tatcagtatc tacgaaaatc ttgcaatgta ttgaattttc atgattttgt
    10621 aggccggata aggcgttcac gccgcatccg gcaagaagct ataagaaaag ggcggagatc
    10681 atctccgccc tttttatttc tgcaatccgc aattatcatc ccgcaaatct atactttttg
    10741 ggtcaacacg taacgcttta caggtgatta tcatggggct ttttgatgaa gttgtcggtg
    10801 cctttctgaa aggcgatgcg gggaaatatc aggctatttt aagttgggtt gaggagcagg
    10861 gcggcattca ggtgctgctg gaaaaactgc aaagtggcgg cttaggggcc attctctcaa
    10921 cctggctgag taatcaacag ggcaatcaat cggttagtgg cgagcaactg gaatcggcgc
    10981 tcggcacaaa tgcggtgtcc gatcttggac aaaaacttgg cgtggatacc agtacagctt
    11041 ccagtttact ggcagaacaa ttgccgaaga ttattgatgc gctctcaccg caaggtgaag
    11101 tgtctccaca agccaataac gatctgcttt ccgcaggcat ggaactgctg aaagggaaac
    11161 tcttccgcta agcaaatggg gagcatgcaa cccgcgttgc atcccccttt tccctcgggt
    11221 gttgtactga tttttgagcg gaatcgcgtt agcatgggtc aggaactaat ctacctgggg
    11281 aactcatggc tatcaaactc attgctatcg atatggatgg cacccttctg ctacccgatc
    11341 acaccatttc acccgccgtt aaaaatgcga ttgccgcagc tcgcgcccgt ggcgtgaatg
    11401 tcgtgctaac gacgggtcgc ccttatgcag gtgttcacaa ttacctgaaa gagctgcata
    11461 tggaactgcc gggcgactac tgcattactt ataacggcgc gctggtacag aaggccgctg
    11521 atggtagcac cgtggcgcaa accgctctca gctatgacga ctaccgtttc ctggaaaaac
    11581 tctctcgcga agtcggttct catttccacg ccctggaccg caccacgctg tacaccgcca
    11641 accgtgatat cagctactac acggtgcatg aatccttcgt tgccacaatt ccgctggtgt
    11701 tctgcgaagc ggagaaaatg gaccccaata cccagttcct gaaagtgatg atgattgatg
    11761 aacccgccat cctcgaccag gctatcgcgc gtattccgca ggaagtgaaa gagaaatata
    11821 ccgtgctgaa aagtgcgccg tacttcctcg aaatcctcga taaacgcgtt aacaaaggta
    11881 cgggggtgaa atcactggcc gacgtgttag gtattaaacc ggaagaaatc atggcgattg
    11941 gcgatcagga aaacgacatc gcaatgattg aatatgcagg cgtcggtgtg gcgatggata
    12001 acgctattcc ttcggtgaaa gaagtggcga actttgtcac caaatccaac cttgaagatg
    12061 gcgtggcgtt tgctattgag aagtatgtgc tgaattaatc agtggacggg caaacagcat
    12121 aaacgcgttt gcccgcttac tgattattgg tttactgcat atacaaccag aaattgcagc
    12181 ggtgcagtaa tgatgtccag cgtaacagtc actggcagta atgctgcgga aacaccatcc
    12241 ggaatgcctc tgggtgaata gtattcataa aatcgcactt taaacggatg atagaacgcc
    12301 atcacctttg agtaatccat gtttttattt ttctcatgga ttgtgccttt taggttttcc
    12361 agtgacctgg tacagtgttg aacaccacag gcaaaaccat aatgcgttgc cagtgccttt
    12421 tcttcttcgt tattccagtg gtagtaaagc gatattgtgc cggtaaattt ctttttcccc
    12481 gcatttaaaa caaatcttgc gtcatcggca acctgaatat tgtgccggtt aagtgctgga
    12541 tcattgagca tcttaacgat gtcatcggca ccatcagtga taaggtaatc cagcgattcc
    12601 cctacaaaaa catagccttt tgttccatta ctatcttttg cctgcgatag cccagtgatg
    12661 gtgtctgact gcgattgata ctttcctgaa ctaccctttg cggtatggga aaccagagca
    12721 tacgagatac agcctgaaag catcagcgcc agtgggaata aaccggcagc cttaaaaaat
    12781 cccttaaaat tcaacttcat gattaatcct ttaaacgagt agctaagccg ttgaaagcat
    12841 actcttcttt ccctcctgtt tagcgcctga tatccctttt cagatttctg cccgacgcat
    12901 gtcatttttt tatgcattgt tctttttgtg atctaaattg tagtacaaca atataagttt
    12961 gtactacatt acgcacacgg caaacgcgaa cgtcatcacg ctggtactac aaagttgccg
    13021 cgttatgcat cgatcggggt aaagtagaga agaacataca gagcacaagg actctccatg
    13081 actctcaata aaaccgatcg cattgtcatt acgctgggta aacagatcgt tcacggcaaa
    13141 tacgtacctg gctcgccact tccggctgag gcggagctct gtgaagagtt tgcaacctcg
    13201 cgcaacatca tccgtgaggt gttccgttcg ttgatggcga aacggctgat tgaaatgaaa
    13261 cgttatcgcg gcgcgtttgt ggcaccgcgt aaccagtgga attacctcga cactgacgta
    13321 ctgcaatggg tgctggaaaa cgactacgac ccacggctta tcagtgccat gagcgaagtg
    13381 cgaaatctgg tggaaccggc gattgcccgt tgggcagcag agcgcgcgac ttccagcgat
    13441 ctggcgcaga ttgaatcggc gctgaacgag atgattgcca acaatcagga ccgcgaagcg
    13501 tttaacgaag cggatattcg ctaccacgag gcggtgctgc agtcggtaca taacccggtg
    13561 ttacagcaac ttagcattgc gatcagttcg ttgcagcggg cggtttttga acgaacctgg
    13621 atgggcgatg aggccaacat gccgcaaacg ctccaggaac ataaggcgct gttcgatgca
    13681 atacggcatc aggacggcga tgcggcagag caggcggcgc ttaccatgat cgccagctcg
    13741 acacgaaggt taaaggaaat cacatgacag ctcgctacat cgcaattgac tggggatcga
    13801 ccaatcttcg cgcctggctt tatcagggcg accactgcct ggagagcagg caatcagaag
    13861 caggcgtcac gcgcctgaac ggaaaatctc cggctgcggt gttagcagaa gtcacgaccg
    13921 actggcgtga agagaatacg ccagtggtaa tggcaggaat ggtcggcagc aatgtcggct
    13981 ggaaagttgc tccgtattta tctgttcctg cccgtttttc gtctattggc gaacaattaa
    14041 cgtctgttgg cgacaatatc tggattattc ccggattatg cgtctctcat gacgataacc
    14101 acaatgtgat gcgcggcgaa gaaacacaat tgatcggcgc gcgaactctg gctccttcct
    14161 ctctttatgt tatgcccggt acgcattgca aatgggtgca ggccgatagc cagcaaatca
    14221 acgattttcg caccgtgatg accggtgaat tacatcattt actgttaaat cactcattga
    14281 ttggcgcagg tttgccgccg caggaaaact ctgccgatgc cttcgcggct ggccttgagc
    14341 gcggccttaa tgcgcccgcc atattgccgc agctttttga agttcgcgcc tcgcatgtgc
    14401 tgggaacact tccccgcgaa caggtcagcg aatttctctc tggtttgttg attggcgcag
    14461 aggttgccag tatgcgcgac tatgtgaccc atcaacacgc catcaccctt gtcgccggaa
    14521 catcgctgac cgcgcgctac cagcaagcct ttcaggcgat gggttgcgac gtgacggcgg
    14581 tggcgggcga cacggcattt caggctggta taaggagcat cgctcatgca gtggcaaact
    14641 aaacttccgc tgatcgccat tttgcgcggc attaagcccg acgaggcgct ggcgcatgtt
    14701 ggcgcggtga ttgacgccgg gttcgacgcg gttgaaatcc cgctgaattc cccacaatgg
    14761 gagcaaagca ttcccgccat cgttgatgcg tatggcgaca aggcgttgat tggcgcaggt
    14821 acggtactga aacctgaaca ggtcgatgcg ctcgccagga tgggttgtca gctcatcgtt
    14881 acgcccaata tccatagtga agtgatccgc cgtgcggtgg gctacggcat gaccgtctgc
    14941 cccggctgcg cgacggcgac cgaagccttt accgcgctcg aagcgggcgc gcaggcgctg
    15001 aaaatatttc cgtcatcggc ttttggtccg caatacatca aagcgttaaa agcggtattg
    15061 ccatcggaca tcgcagtctt tgccgttggc ggcgtgacgc cagaaaacct ggcgcagtgg
    15121 atagacgcag gttgtgctgg ggcgggctta ggcagcgatc tctatcgcgc cgggcagtcc
    15181 gtagaacgca ccgcgcagca ggcagcagca tttgttaagg cgtatcgaga ggcagtgcaa
    15241 tgaaaatcac caaaattacc acgtatcgtt tacctccccg ctggatgttc ctgaaaattg
    15301 aaaccgatga aggcgtggtc ggttggggcg agcccgtgat tgaaggccgc gcccgtacgg
    15361 tggaagcggc agttcacgag ctgggtgact atttgattgg tcaggatcct tcgcgcatca
    15421 atgacttatg gcaagtgatg tatcgcgccg gattttatcg tggcggtcca atcctgatga
    15481 gcgccattgc cgggatcgac caggcgttat gggatatcaa aggcaaagtg ctgaatgcgc
    15541 cggtctggca actgatgggc ggcctggttc gcgacaaaat taaagcctac agttgggtcg
    15601 gcggcgatcg tccggcggat gttatcgacg gcattaaaac cctgcgcgaa atcggcttcg
    15661 ataccttcaa actgaacggt tgtgaagaac tggggctaat tgataactcc cgcgcggtag
    15721 atgcggcagt caacaccgtg gcacaaattc gtgaagcttt tggcaatcag attgagtttg
    15781 gtcttgattt ccacggtcgc gtcagcgcgc caatggcgaa agtgctgatt aaagaactgg
    15841 agccgtatcg cccgctgttt attgaagagc cggtgctggc ggaacaagcc gaatactacc
    15901 cgaaattggc ggcacaaacg catattccac tggcggcagg tgagcgcatg ttctcacgtt
    15961 tcgattttaa acgcgtgctg gaggcaggcg gtatttcgat tctgcaaccg gatctctccc
    16021 atgcaggcgg tattaccgaa tgctacaaaa ttgctggaat ggcagaagcc tatgatgtga
    16081 cccttgcgcc gcactgtccg ctcggaccga ttgcactggc ggcttgcctg catatcgact
    16141 ttgtttccta taacgcggta cttcaggaac aaagtatggg cattcattac aacaaaggcg
    16201 cggagttact cgactttgtg aaaaacaaag aagacttcag tatggttggc ggcttcttta
    16261 aaccgttaac gaaaccgggc ttaggtgtgg aaatcgacga agctaaagtt attgagttca
    16321 gtaaaaatgc cccggactgg cgtaatccgc tctggcgtca tgaagataac agcgtagcag
    16381 agtggtaatt cctgccacgt aagcccctca tcgggcacta aaacagcaat acaaaaatat
    16441 aaccctctgt aaattacagg gcatggtgag cggcttcgct atgcccaaaa tctggagaaa
    16501 gctcgctatg gatattcccg ttaatgcagc aaagccgggg cgtcggcgtt atctgacgct
    16561 ggtgatgatc tttattacgg tggtcatttg ttatgttgac cgcgctaacc tggccgtggc
    16621 ttccgcccat attcaggaag agttcggcat tacgaaagcg gaaatgggct atgtattttc
    16681 ggccttcgcc tggctttata cgctatgcca gatccccggc ggttggtttt tagatcgcgt
    16741 aggttctcgc gtgacttatt ttattgcgat atttggttgg tcagtggcga ctttattcca
    16801 gggctttgcc acgggattaa tgtcattaat tggtctgcgt gcgataaccg gtattttcga
    16861 agcgcccgct ttcccgacca ataaccggat ggtgaccagc tggttcccgg aacatgaacg
    16921 cgcttccgcc gttggttttt atacgtctgg tcagtttgtc ggtctggcgt ttttgactcc
    16981 gctgctgatc tggattcagg agatgttgag ctggcactgg gtgttcattg tcactggtgg
    17041 tatcggcatt atctggtcgc tgatttggtt taaggtttat cagccgccgc gcctgaccaa
    17101 aggtatcagc aaagctgaac tggattacat tcgtgatggc ggcggtctgg tggatggtga
    17161 tgcgccggtg aagaaagagg cgcgtcagcc gttaacagcc aaagactgga aactggtgtt
    17221 ccatcgtaaa ctgatcggcg tttatcttgg gcaatttgcg gtggcttcta cactgtggtt
    17281 tttcttaacc tggttcccga actatttaac ccaggaaaaa ggaatcacgg cgctgaaagc
    17341 aggctttatg accacggtgc cattcctcgc ggcgtttgtc ggcgtcctgc tctctggctg
    17401 ggtcgcggat ctgctggtac gtaagggctt ttcactgggc tttgcgcgta aaacgccgat
    17461 tatctgcggc ttgctgatct ccacctgcat tatgggcgct aactacacca acgatccgat
    17521 gatgattatg tgcctgatgg cgctggcatt cttcggcaac ggttttgctt cgattacctg
    17581 gtcgctggtc tcttctctgg caccgatgcg cctgattggt ttaaccggcg gcgtgtttaa
    17641 cttcgccggt ggtctgggcg gcatcaccgt tccgctggtg gtggggtacc tggcgcaggg
    17701 ttacggtttc gcacctgcac tggtttatat ctccgccgtc gcgttgattg gcgcgctctc
    17761 ttacatcctg ctggtgggcg atgtgaagcg cgttggctaa tctttcaact gtggaatatg
    17821 cgccatacca ctgcgcataa tccgctttct taaagccggt gcggtcaggc atcggctttt
    17881 tactatcttg ccgaagagtt ttaaacgcag tttgcgggtg gcgcgccagt aagcggtatt
    17941 gagcttctct ggctgcttca gtaacaccga acgcagaatg tctgcgctat ccagcgcata
    18001 gctaatcccc tccagcgaac tggcgctgat aaatcctgcc gcttcgccaa tcagaaaggc
    18061 gttgtcctta ccgcagacaa aatcctgcca gcgtgaggga aacagcaccg tgcatttttc
    18121 gcttttcacc gccttaccaa actggaactg aaaggcgctc attttctctt tcagcgtcgt
    18181 gaaacgcgtc tgaccgtctt tcattggata agcaccgcca aagataaaat aaccgtcttt
    18241 gctgatactc catgaataac agttagttat cgaattatca aagatgcagg agtagaacgg
    18301 caccggatgt ttctctgcga accactgctg gatagcgaca tatttacgga tttgatgatc
    18361 cgggtagaga tgtcgccgca ccatcgagtt tgcgccatcg gcaccgacca gatagcgggc
    18421 agtaatatgt tgctcccagc cgtctgcacg aaaaatgaca tgccatttat catcctcacg
    18481 ccagattttt cgacacaggc tgtcgtgata aacctcaacg ctggcgggga tcagcgattt
    18541 catccacaag tcgaaagcat ggcgattaat attgatatag cttcgctggt agttacgcgt
    18601 gagcgatgcg gcgacgtcga cagttttgac gctgaaaatc tgcgggttgg cgatcacatc
    18661 gacaggaagc gtcagtccat cgcgaataaa agatcgctgc gcgtccggtg ccagcagacc
    18721 gctgcaaggt ttgctgaaac cttcagtacc acactggtgc tttttatcca gcgcgatcac
    18781 ctgcattttg cctgctaatt ttcgcgccaa cgccgatcct gccgggccga ggccaataat
    18841 cgccacgtcg aaatgttcca tcttttcact cacattcatc acgatggcaa aagactattg
    18901 tctgaaagtg aagtccttgt gaagtatgat ggcgggaccc gttttgtatc aggaccgcgc
    18961 catgaaacag ataacctttg ctccccgtaa tcacctgctc accaatacca atacctggac
    19021 gcccgacagc cagtggctgg tatttgacgt gcgtccttct ggggcgtcgt ttaccggcga
    19081 gaccattgag cgtgtgaata tccataccgg cgaggtcgag gttatctatc gcgcgtcaca
    19141 gggcgcacac gtcggcgtgg tgaccgttca tccgaagtca gagaaatatg ttttcatcca
    19201 tggcccggaa aatcctgatg aaacatggca ttacgatttc catcatcgac gcggagtcat
    19261 tgctgaaggc ggcaaggtga gcaatctcga tgcaatggat attaccgcgc cgtatatgcc
    19321 aggagcgctg cgcggcggca gccatgtgca tgtctttagc ccgaacggtg aaagggtgag
    19381 ctttacctat aacgaccatg taatgcatga actcgatccg gcgctggatt tgcgaaacgt
    19441 cggtgttgct gcgccgtttg gcccggtcaa cgtacaaaag cagcatccgc gtgaatacag
    19501 cggtagccac tggtgcgtgc tggtgagtaa aaccacgccc acgccgcagc ctggcagcga
    19561 tgaaatcaat cgtgcttatg aagaaggatg ggtaggaaat cacgcgctgg cgtttattgg
    19621 cgacacactt tcgccaaagg gcgagaaagt gccggagctg tttatcgttg agttaccgca
    19681 agatgaagct ggctggaaag cggcaggtga tgcgccgtta agtggaacgg aaaccaccct
    19741 gcccgcgcca ccgcgcggcg tcgtgcagcg acgtttaacc tttacccacc atcgggctta
    19801 tccggggtta gtcaacgtcc cgcgccactg ggtgcgctgt aatccgcagg gtacgcaaat
    19861 cgcgttttta atgcgtgatg ataacggcat tgtgcaactg tggcttatct cgccacaggg
    19921 cggcgagccg cgccagttaa cccataacaa aacggatatt cagtctgcat ttaactggca
    19981 tccgtcagga gaatggttgg gctttgtgct ggataatcga attgcttgtg cccatgcgca
    20041 aagcggcgag gttgagtatt taaccgaaaa ccacgccaat ccaccttctg cggacgccga
    20101 ggtcttctcg ccggatggtc aatggctggc gtggatggaa ggtggccagc tgtggatcac
    20161 cgaaactgat cgctaaatca gttatcaggc attctggccg gtggaataac cgcattggtg
    20221 gcttgcgcgt tggcttcgct tttttcaacg cgtgagcgca ccgagctgtc tttgcggaac
    20281 acatcccacg gcagtaaaag cgtgtccatc acagcggtaa acggcatatc gagaatcgcc
    20341 agcgacttag tgccccagtt ggtttcatcg tcgccaatca tggttgcgct ggcgcgggtg
    20401 ccgggatacg ttccttcttt ccctccggta tgggacatca cgctggagca tcctccgagt
    20461 aatgtcattc cgctgcatat catgaacgct aacaacacat ttcttatcat aataaattca
    20521 tctgttgatc gtgggtgttg gcctgatgag ttatagcgat cccttgctga aaataacatc
    20581 atcattacgt cgcactgtgg cggctatcgc actttaacgt ttcgtgctgc cccctcagtc
    20641 tatgcaatag accataaact gcaaaaaaaa gtccgctgat aaggcttgaa aagttcattt
    20701 ccagacccat ttttacatcg tagccgatga ggacgcgcct gatgggtgtt ctggctacct
    20761 gacctgtcca ttgtggaagg tcttacattc tcgctgattt caggagctat tgattatgcg
    20821 taactttgat ttatccccgc tttaccgttc tgctattgga tttgaccgtt tgtttaacca
    20881 cttagaaaac aaccagagcc agagtaatgg cggctaccct ccgtataacg ttgaactggt
    20941 agacgaaaac cattaccgca ttgctatcgc tgtggctggt tttgctgaga gcgaactgga
    21001 aattaccgcc caggataatc tgctggtggt gaaaggtgct cacgccgacg aacaaaaaga
    21061 gcgcacctat ctgtaccagg gcatcgctga acgcaacttt gaacgcaaat tccagttagc
    21121 tgagaacatt catgttcgtg gcgctaacct ggtaaatggt ttgctgtata tcgatctcga
    21181 acgcgtgatt ccggaagcga aaaaaccgcg ccgtatcgaa atcaactaat tccctaaggc
    21241 cgcctggcgc ggcctgacat ctccatgctc gccgtcaggg agcatatgcg aatcttcgga
    21301 tttgcaggta cttactcgct tcttagaagg agaaatgact atgcgtaact tcgatttatc
    21361 cccactgatg cgtcaatgga tcggttttga caaactggcc aacgcactgc aaaacgccgg
    21421 tgaaagccag agcttcccgc cgtacaacat tgagaaaagc gacgataacc actaccgcat
    21481 tacccttgcg ctggcaggtt tccgtcagga agatttagag attcaactgg aaggtacgcg
    21541 cctgagcgta aaaggcacgc cggagcagcc aaaagaagag aaaaaatggc tgcatcaagg
    21601 gcttatgaat cagccattta gcctgagctt tacgctggct gaaaatatgg aagtctctgg
    21661 cgcaaccttc gtaaacggtt tactgcatat tgatttaatt cgtaatgagc ctgaacccat
    21721 cgcagcgcag cgtatcgcta tcagcgaacg tcccgcgtta aatagctaac tagctattct
    21781 ctttgccccg ccattctggt ggggcttttt ttgtcttact tactcaacaa tgcgttaaat
    21841 ttcgactgtt taagatattt cggcacgttc tttcccgtga taatgtgcgc tataaggcaa
    21901 atctgcttca ccgattcagc cataatccgt agcaattaaa aatgttatct caagagaaat
    21961 ggacgatgag tgatatagca ttaacggtca gtattctggc tttggtggca gtcgtcggtt
    22021 tgtttatcgg caatgtcaaa tttcgcggca taggattagg tattggcggc gtgctgtttg
    22081 gtgggatcat cgtcggccat tttgtttctc aggcggggat gacattaagt agcgatatgc
    22141 tgcatgttat tcaggaattt ggcctgatcc tgttcgttta tactatcggg attcaggtag
    22201 ggccgggctt ctttgcctca ttgcgcgtct ccggattacg cctcaacctg tttgctgttc
    22261 tgatcgtcat catcggtggt ctggttaccg ccatcctgca taaactgttt gatattccac
    22321 tgccggtagt gctggggatt ttctccggtg cggttaccaa tacgccagcg ctgggggcag
    22381 ggcagcagat tttgcgcgac ttgggtacac caatggaaat ggtcgatcag atggggatga
    22441 gttacgcgat ggcgtatcca ttcggcattt gcgggatttt gttcaccatg tggatgttgc
    22501 gggttatttt ccgcgtcaat gtcgagacag aagctcagca gcacgagtct tcacgcacca
    22561 atggcggcgc gctgatcaag actatcaata ttcgcgttga gaaccctaac ctgcatgatt
    22621 tagccattaa agatgtaccg attctcaacg gcgacaaaat tatctgctcg cgtctgaaac
    22681 gcgaagaaac cctaaaagtt ccttcgccag ataccattat ccaactgggc gatttgctgc
    22741 atctggtggg gcagccagcg gatttacata atgcgcaact ggtgattggt caggaggtcg
    22801 atacttcgct gtccacgaaa ggcactgatt tgcgcgtcga gcgtgtggtg gtcaccaatg
    22861 aaaacgtgct cggaaaacgt attcgcgacc tgcactttaa agaacgctat gacgttgtta
    22921 tctcgcgcct gaaccgtgcc ggggtcgaac tggtcgccag tggcgatatc agcctgcagt
    22981 tcggcgatat cctcaatctg gtggggcgtc cgtccgcaat tgatgccgtt gccaatgtgc
    23041 tggggaatgc gcagcaaaaa ctgcaacagg ttcagatgct gccagtgttt attggcatcg
    23101 ggcttggcgt attgttaggt tctattcccg tctttgtgcc tgggttcccg gccgcgttga
    23161 aactggggct ggcgggcggt ccgctgatta tggcgttgat cctcgggcgt atcggcagta
    23221 tcggcaagct gtactggttt atgccgccaa gtgccaacct cgcgctgcgg gagctgggga
    23281 tcgtgctgtt cctctcggtc gttggtctga aatctggtgg agattttgtg aataccctgg
    23341 tcaatggcga agggctaagc tggattggtt atggtgccct gatcaccgcc gttccgctga
    23401 ttactgttgg cattctggcg cggatgttag ccaaaatgaa ttacctgacc atgtgcggga
    23461 tgctggcagg ttccatgacc gatcctccgg cgctggcgtt tgctaataat cttcatccaa
    23521 ccagcggtgc ggcggcgctc tcttacgcca ctgtctatcc gttagtgatg ttcctgcgca
    23581 ttatcacccc ccaattactg gcggtgctct tctggagtat cggttaactc ttttccgggt
    23641 ggagtcgccg taaatggtac tccacctggt attcgctggc atttctgaac atcactgaat
    23701 aattcaaaaa ctccccgctc tcgctatatg acaacgaggt gatccgcagc agtggcgtgt
    23761 gttccgctac cttcatttgc cgcgccagca gtctatcggc gagcactggc gtcaggcttt
    23821 cataattacc gccaatcaaa atcccgcact cttgttcaat atattcaaac ttcgaccctt
    23881 ccagatgttg cagcgaaaga ttacggaaca gttttaccgg catatagctg tcttccagca
    23941 tcagcggttt cccttccaca aaacgaacgc gacgggagaa gtagatctgc tcgttgattt
    24001 gaatccgtaa ctggctggca atggccggag gcgcaggcat gatttcaaaa atcagcacct
    24061 ggctggtgac cgtttttccc tgccgtttta acacctccac cagcccggtc aggctggcgg
    24121 tttgatgcag tacatctttg cgcaccaggt aagtgccgct gccgtggcgg cggaccacca
    24181 atccccacgc taccagcagg tcaatggctt tacggatggt catccgcgat accgcaaatt
    24241 cttccgccaa ctttttttca ccgggaagaa ggctatttag cgtgaaatct gcggaattaa
    24301 gtcgaattct taaccgctcc gcaatgcttt tgtagatcat ctctagatct cttttttgcc
    24361 tgcaagcacg ggtaatgcgc ccacaagttc tgcttaatcg attgaaaaaa catctttttt
    24421 ttaaagatgt gttcgatgct gtgtacctgt gctcacagat gtctactttt tcgcgaaaac
    24481 gtagatctct accgcccaac gaaaagcatg aaagcgatca cgaatcccat tgggtcgtca
    24541 tgttctgccg atcgcatctt cctatcctcg ctccaggcct gccgcataac caatcaggct
    24601 tcctacttac agaattgaga aaagaggatg tggaaatgct cagtcaaatt caacgctttg
    24661 gcggcgcgat gttcacgcca gtgctgctgt ttcccttcgc cgggattgtg gtgggtcttg
    24721 ccatcttgct gcaaaacccg atgtttgtcg gggaatcact gaccgatccg aacagtttat
    24781 tcgcgcaaat cgtacacatt attgaagagg gcggttggac ggtgttccgt aatatgccgc
    24841 tgatttttgc tgtcggttta cccattggcc ttgctaagca agcgcagggg cgtgcttgtc
    24901 tggcggtgat ggtgagtttc ctgacctgga actatttcat caacgcgatg ggaatgacct
    24961 ggggaagcta cttcggcgtc gatttcactc aggacgcggt ggcaggtagc ggtctgacaa
    25021 tgatggccgg gattaaaacc ctcgatacca gcattatcgg cgcaattatc atttccggca
    25081 ttgtgacggc gctgcataac cgtctgttcg ataaaaaact gccggttttt ctcgacattt
    25141 tccaggggac gtcttatgtg gtgattatcg ccttcctggt gatgatcccc tgtgcctggc
    25201 tgacgttgct cggctggcca aaagtacaaa tggggattga atctctgcaa gcgttcctgc
    25261 gttcggcggg tgcgcttggg gtgtgggttt acaccttcct cgaacgtatt ctgatcccaa
    25321 ccggtttaca ccacttcatc tacggaccgt ttatctttgg tccggcagct gttgaaggcg
    25381 gcattcagat gtactgggcg cagcatctgc aagagttcag tctgagcgcc gagccgctga
    25441 aatcgttgtt cccggaaggc ggttttgccc tgcacggtaa ctcaaaaatc tttggtgccg
    25501 tgggcatttc tttagcgatg tacttcactg ccgcaccgga aaatcgggta aaagtggcgg
    25561 gcttgctgat tcccgcaacc ttaaccgcca tgctggtggg aattaccgaa ccgctggaat
    25621 ttaccttcct gttcatttca ccgttgctgt ttgcggtaca cgccgtgctg gcggcctcaa
    25681 tgtcgaccgt gatgtatctc tttggtgtgg tgggcaacat gggcggaggt ctgattgacc
    25741 aggttttacc gcaaaactgg atcccgatgt tcagcaacca cgcggatatg atgctgaccc
    25801 aaatcgccat tgggttgtgc tttaccctgc tgtacttcgt ggttttccgc accctgattc
    25861 tgcagttcaa catgtgcacg ccgggacgtg aagatgcgga agtgaaactc tactcaaaag
    25921 ccgaatacaa agcctcgcga ggccaaacca ccgcggcaga gccaaaaaaa gagctggatc
    25981 aggctgccgg tatcctgcaa gccctgggcg gggtcggcaa tatctccagc attaacaatt
    26041 gcgcgacgcg tctacgcatt gcactgcatg acatgtcaca aacgctggat gacgaagtct
    26101 ttaaaaagct gggagcgcac ggcgtcttcc gtagtggcga tgccattcag gtgatcattg
    26161 gtctgcatgt atcccaattg cgtgaacagc tcgatagctt aattaattct catcaatcag
    26221 cagaaaatgt tgccattacg gaggcagtat aatgaccaaa ttctcagtgg ttgtcgcagg
    26281 cggtggaagc acctttacgc caggcatcgt gttgatgctc ctggcgaatc aggaccgttt
    26341 cccgcttcgt gcactgaaat tttatgataa cgatggcgcg cggcaggaag tgattgccga
    26401 agcctgtaaa gtcatcctta aagaaaaagc gccggacatt gcgtttagtt acaccaccga
    26461 tcctgaagta gcgttcagcg acgttgattt tgtcatggcg cacatccgcg tcggcaagta
    26521 cccgatgcgc gaactggatg aaaaaatccc actgcgccac ggtgttgttg gtcaggaaac
    26581 ttgcggaccc ggcggaatag cgtacggcat gcgttccatt ggcggcgtcc tggaactggt
    26641 ggattatatg gaaaaatatt caccaaacgc ctggatgctc aactactcca acccggcagc
    26701 aattgtcgca gaagccacgc gtcgtctgcg cccgaatgcg aaaatcctca acatctgtga
    26761 catgccaatc ggtattgaaa gccggatggc gcaaattgtt gggctgcaag atcgcaaaca
    26821 gatgcgcgtg cgctactacg gcctgaacca ctggtggtcg gcgatatccc gcagtttcag
    26881 aaagggttaa tgagtcagca agtggcggtg gaaaaactgg tcgtggacgc ctgggaacag
    26941 cgttcatatc agcacctgtg gcaggcgatt acgttgtcga aaacggtacc gagcgcctcg
    27001 gtcgccaaag ctattctgga tgagttgctg gaggccaaca aagcgtactg gccagagtta
    27061 cgttaattgt gtggactggc atccgttggg tgctggtccg tattctttag cacggctgac
    27121 ggcgcataac caaatttatg tttaaaggct ttgctgaaat gaaaggagtc gaagaagttg
    27181 agcgtatcag cgacttcgcc aaccgagttt ccttgttgct ttaatagcga tagcgccaga
    27241 tccagacgcg catcgaggta atattctttc ggcgtcttgc cggtatagcg taaaaacaga
    27301 cgacgcaacc aggcttcgct acaggggatc gtggcagcca tatcagctac gctccagcgt
    27361 tgttgcagac tggcatgtaa agtggcaatt aatttttcaa tttgccgccg ttgtggatct
    27421 tttttaccat ctgcgtacat aagacaaatc cattgataaa taattttcgt cagaaaagcg
    27481 actgccagat tattttttat tgcttctggt gaagttatta actcagcaac ttcggtgagt
    27541 tcctgattat aaatttcgcc attataaata acgctttgct gaccaacggg aatatccatc
    27601 atactggtgg gggtaaattc catccagtac tgttcccaga ctaaaccttc acagtgataa
    27661 gagtgaatat ccattggctt taagaatata atacagttgc catgcagtgt tatttgttcg
    27721 ccattcttta aaagaatttt cccacatcct tgtacggtat acaccgcaac ccatgagttg
    27781 gcgataagcg gtttcttctt gttccgcggc cagatcaccc ggtaatgctc gtcggcgtag
    27841 gtatgccaga gcgagatcag agaaattccg ctggagatca cattttcgtt aatcaataag
    27901 ggaatattgt acggagtttc gttttttaca tctgaacttt gcaattttcc attcatttta
    27961 atatccctcc ggcgaattat gctcacaacc tgaacttaac agaaaacaat ccgttcaggg
    28021 atacgacgtt tgtattttaa gaatctgact gcctgacccg acgcatttta cctctcccta
    28081 tattcatgcg tccgggacat aaacataaat aagggctatg agatgaattc gttacaaatc
    28141 ttgagttttg tcggttttac gctgctggtg gcggtgatca cctggtggaa ggtccgcaaa
    28201 acagataccg gatcgcaaca aggctatttt cttgccggac gttcactaaa agcgccggtt
    28261 attgccgctt cgttaatgct aaccaacctt tccacggaac aactggtcgg cctttccggg
    28321 caggcctaca aaagcggcat gtcggtgatg ggctgggaag tgacttcagc ggtgacgctg
    28381 atcttcctcg cgctaatctt tttaccgcgc tatctgaagc gcggcattgc caccatcccc
    28441 gattttctgg aggaacgtta tgataaaacg acgcgtatta tcatcgactt ctgcttcctc
    28501 attgccaccg gcgtctgctt tctgccgatt gttctctact ccggcgcgtt ggcgctcaac
    28561 agcctgtttc acgtcgggga atcgctacag atttctcacg gtgcggctat ctggctactg
    28621 gtaattttgc ttggtctggc gggaattttg tatgcggtga tcggcggact gcgcgcaatg
    28681 gcagtggcgg actccatcaa cggtattggg ctggttatcg gcgggttgat ggtgccggta
    28741 tttggcctga tcgcgatggg caagggcagc tttatgcagg gcattgagca actcaccacc
    28801 gttcacgccg agaaattaaa ctcaatcggt ggcccgaccg atcccttgcc gattggcgcg
    28861 gcatttaccg gtttgattct ggtgaacacc ttttactggt gtacaaatca gggcatcgtg
    28921 caacgcacgc tggcgtcaaa aagcctggcg gaagggcaaa agggggcgct gttaacggcg
    28981 gtgctgaaaa tgctcgaccc gctggtactg gtgctgccag ggttgattgc gtttcatctg
    29041 tatcaggatt taccgaaagc cgacatggcc tacccgacgc tggtcaataa cgttctgcca
    29101 gtgccaatgg tgggtttctt cggcgcggtg ttatttggtg cggtgatcag taccttcaac
    29161 ggctttctga atagcgccag tacgttattc agtatgggta tttaccgtcg catcattaac
    29221 cagaatgccg agccgcagca gctggtcacc gtcgggcgca aatttggttt ctttatcgct
    29281 atcgtttcgg tgctggtcgc gccgtggatc gccaacgcgc cgcaggggct gtatagctgg
    29341 atgaaacagc tcaacggcat ttacaacgtg ccgctggtta ccatcatcat tatgggcttt
    29401 ttcttcccgc gcatcccggc gctggcggca aaagtggcga tggggattgg cataatcagc
    29461 tacatcacca tcaactatct ggtgaagttc gacttccatt tcctctatgt gctggcctgt
    29521 acgttctgca tcaacgtggt cgtgatgctg gtgatcggtt ttatcaaacc gcgcgccacg
    29581 ccgttcacct tcaaagatgc gtttgcggtg gacatgaaac cgtggagaaa cgtcaagatc
    29641 gcgtcaattg gcatcctgtt cgcgatgatt ggcgtctatg ccgggctggc tgaattcggc
    29701 ggctacggta cgcgctggtt agcgatgatc agttatttca ttgccgccgt agtgattgtc
    29761 tacctgattt ttgacagctg gcggcatcgt cacgacccag ccgtaacctt tactcccgac
    29821 gcgaaggata gcctatgaaa cgccccaatt ttctgttcat catgaccgat acccaggcca
    29881 ccaatatggt cggttgctat agcggtaaac cgctgaatac gcaaaatatt gatagtctgg
    29941 cggcggaagg tattcgcttt aattccgcct acacctgttc accggtttgt acgcctgcac
    30001 gcgccggact atttaccggt atctacgcta accagtccgg cccgtggacc aacaacgtcg
    30061 cgccaggcaa aaacatctcc actatggggc gctactttaa ggatgccggc tatcacacct
    30121 gttacatcgg caaatggcat ctcgacggtc atgactattt cggcactggc gagtgtccgc
    30181 cggagtggga cgctgattac tggttcgatg gggcgaacta ccttagcgaa ctgacggaaa
    30241 aagagatcag cctgtggcgc aatggcctaa acagcgttga ggatttacag gcgaaccata
    30301 tcgacgaaac cttcacctgg gcgcaccgca tcagcaatcg ggcagtagat tttctgcaac
    30361 agcccgcgcg cgccgaggaa cccttcctga tggtggtttc gtatgatgag ccgcatcacc
    30421 cgttcacctg tccggtggag tatttagaga aatacgctga tttttactac gatctgggcg
    30481 agaaagctca ggatgacctg gcgaacaaac cggaacatca ccgcttatgg gcgcaggcga
    30541 tgccatcgcc agtcggtgat gacgggcttt atcaccatcc gctctatttt gcctgtaatg
    30601 actttgttga tgaccaaatc ggacgggtca tcaatgcctt aacgccagag caacgtgaaa
    30661 atacgtgggt catttatact tccgatcacg gcgaaatgat gggcgcacat aagctgatca
    30721 gtaaaggagc ggcgatgtat gacgacatca cccgtattcc gctgatcatc cgttcgccgc
    30781 aaggggagcg gcgacaggtc gatacgccag tcagtcatat cgatttactg ccgacaatga
    30841 tggcgctggc agatattgaa aaaccagaga ttctgccggg ggaaaatatc cttgccgtga
    30901 aagagccacg cggtgtaatg gtggaattta accgctacga gattgagcat gacagctttg
    30961 gcggttttat tccggtgcgt tgctgggtga cggatgactt taaactggta ctcaacctct
    31021 tcaccagtga tgaactttac gatcgccgta atgacccaaa tgaaatgcat aacctgatcg
    31081 atgatatccg ttttgcagac gttcgcagca aaatgcatga cgccttattg gattacatgg
    31141 acaaaattcg cgatccgttc cgcagttacc aatggagcct gcgtccgtgg cgtaaagatg
    31201 cactgccgcg ctggatgggg gcatttcgtc cacgcccaca agatggctat tcgccggtgg
    31261 tacgtgacta tgacaccggc ctaccgacgc aaggagtgaa agtggaagag aaaaaacaga
    31321 agttctgacg ataatgcccg ctgatgaaca cccggcgggc attattcagg ctgcttaaac
    31381 cagtgaaccg ccggtcagga aggaaaaaat caggaatatc agcaaaatgc tgcacacgat
    31441 gccgatacca aaaagcaagg tatggaaagc cagcgtcccg ccgccaaaaa ccagcgcgcc
    31501 gcgaatgccc cagcgatttt ccttacgcat accgccaata aatgcgcaaa gtagcgcagc
    31561 aatggccagt gcgataccgc tttgatcgag gattttatcg acatctacgt cctctttttt
    31621 ctccacggtg gtaatttttt cgccttttat tccggcgaga agacctcttt taatttcagc
    31681 cgttttgtcc gccacgatgc tttcaaacgt tggcggtggt gcggaaaaag ggccgaatga
    31741 aaagtggata atccccatca ttaaagcgat ggcaccgaac agcatgccca atgaactgat
    31801 tttattttga gctatgatac ccatctgacc tccttgtagc ggatggcgaa agcatatcag
    31861 gaaacgggcg gacgaaattc ttaaaaaaaa accgctaaag gtagagcgtt agtgaaattg
    31921 caaaggagca acaatgaaaa tttcccgcct tggagaagcg cccgattacc gcttctcgct
    31981 ggctaatgag cgtacttttc tggcgtggat ccgtaccgca ctaggatttc tggcggcggg
    32041 ggttgggctt gatcaacttg cgccagattt cgccacgccc gtcattcgtg aactgctggc
    32101 gttgttgttg tgtctgtttt ccggcgggtt agcgatgtat ggctacctgc gctggttacg
    32161 caatgaaaaa gcgatgcgtc tgaaagagga cctgccctac accaacagct tattaatcat
    32221 tagcttaata ttgatggtcg tggcggtgat cgttatggga ctggtgttgt atgccggata
    32281 gccgcaaagc cagacgcatt gccgacccag gactgcagcc ggagcgcaca tcactggcgt
    32341 ggtttcgtac catgctgggc tacggcgcgt tgatggcgtt ggctatcaaa cacaactggc
    32401 accaggcggg catgttattc tggatttcga ttggcattct cgccatcgtg gcgctgatcc
    32461 tctggcacta cacccgtaat cgcaatttaa tggatgtcac gaatagcgat ttttcccaat
    32521 ttcacgtagt ccgtgacaaa tttttgatct ccctcgcggt gttatctctc gcaatactgt
    32581 ttgctgtaac gcatatacat caacttatcg tatttattga gagagtcgca tgacaggaag
    32641 tcaggtcatt gatgctgaag aggacaggca taaactggtt gtagagtaca aggatgctct
    32701 gcaacctgcc gatttttatc acaatttcaa acagcggggc atccgctccg tacagctcat
    32761 tccctatctt gaatttgatg accggggcga tctgacggcc gcttcggtga cagcagagct
    32821 gtgggggaaa tttttaattg ccctgttcga gtgttgggtg cgggcagata tcagtcgtat
    32881 ctcgattgag ctttttgacg ccaccttaca aaaatggtgt ggcagcgaaa acccgcaacc
    32941 gcgccgtgac tgccaggcgt gcgactggca tcgcctgtgc ccgcacgctc gccaggagac
    33001 gccagacagc gtgctttgtg cgggctatca ggcgttttat tcttactccg caccgcatat
    33061 gcgggtgatg cgtgatctga taaagcagca tcgttctccc atggagctga tgacgatgct
    33121 gcggtgacgt gcgcttaaac gggctgcccc tgatgcgaca tccgcgtcgc cagcggcagc
    33181 cagcacagca cgatcaacaa tcccattaag gtcatcaaca accccaggct gccctgaccg
    33241 gtttgcggca acatcgcaga gagcgacgcc agcacgccgg aaccaatgtt ttgcagaccg
    33301 ccgaccagcg cgccagccgt gcccgccagg aaggggaacg gctccatcgc gccgctggtc
    33361 gccagcggaa acagcatccc ggcaccgaaa aagaacagcg cggcgggaac gagcagcgtc
    33421 cagacattca tcacgccaaa ccagtcgggg atccacatca gcaagccagc cagcaggcag
    33481 cagataaccg actgccacat taacgtggag aagcgtttat tgggacgtcc ggcaaaccat
    33541 gcgccaaaaa acgctgccgg aatcggcaga ataaacaaaa tactgaccgt catactgctc
    33601 agccctaaca ctgcgcccat cagcacgccg gagcaggctt caaaggcggc aatcccggcc
    33661 agaccgccaa tcagcatcag caaataacag ttaaaaccgc tgttaccgaa aagcgtttta
    33721 taactggtaa gcaggcgcgt gcgcggtgca tcgaccggac gcgtttccgg catccagcgg
    33781 gccatactga aggtcacacc agcacaaaga accaacaaaa agagataaca ggcgcgccag
    33841 ttccacatcg tatccagcag accgccgatt agcggtgcga gcaacggact gacgagaatc
    33901 cccatgttta acaggctgtt agcatggcgc aactgtgtcc gttcatataa atctcgcggc
    33961 aaagtacgcg ccattacgcc gccaacgccg gtgcccatcc cctgcatcgc gctggcggca
    34021 atcaacaccg tcaaactgga ggtcgtgacc gcgaccagcg ttgccagcat aaaaatggac
    34081 attccgacga ggatcaccgg tcggcggccc acgcggtcgg aaatcgggcc ataaaacagc
    34141 tgtgagacac cgtaagtcag cagataagcg cccattacgc tctgcaccgc cccttcacgg
    34201 acgttgagat cgcgcgccat atcggcaata gctggaatat aaatggtttg cgccatctga
    34261 ccgacggcca cgagtaatac caacatcaat aacaaattga cgtttctttg ccttttcatt
    34321 atcacggatg cttttataaa aataattaga ataagtcact acgcattccc ataaatgcgg
    34381 gagaggaatc tatcacattg atgaatgtta gccagatata cgccaggaat ggggaattgt
    34441 ttagcggcag aatatgtaaa caaaagcggc aataaatgtt gccgggaggg attacgtgcg
    34501 aactgtaacc ctctccccaa aggggcgagg gggaaaccac cgttcccttt cagaaaatga
    34561 gctaaacatt gcacgggacg gtcccctcgc ccctttgggg agagggctag ggtgaggggc
    34621 acaggcagcg caggtgcaaa cattctgctg gtagaagaag cccccaactc caacccgcac
    34681 gctaaatata aaaggggagc ggttacccgc tcccctttgg tgcgacttga atctgaatta
    34741 cttcaggtat ttcagaacag catcaagcag ttgcagtgct gcaacaatga gtttgaggat
    34801 aagaatggcg atatccacca ggctcatacg cgtctcctgt ggttcaggag cactgccagc
    34861 tgacgtacct ttccgctgcc tgttgccagc tgttgtgcac gcggtatctt aaggagaaac
    34921 gttgtgcttg ttgacataac acagtgtgct cacgcggggt taaggcgctg acggcaccac
    34981 ccgtttcagc caggacttag tgcgccgggt aacgatgcgg cccccgcata acacattgcg
    35041 tacagtgata ttatagaagt ttactgtata aataaacagt aatatttgga caaaacgcaa
    35101 actgtgatcg atactcaact atgccctaaa tacgtcaaaa ttcgtgccga aattgcgcgt
    35161 tctgcgcgga acacgtatac tttcagtgtt gacataatac agtgtgcttt gcggttacca
    35221 gccgcaggcg gctgatgaaa cctcgctccg gcggggtttt ttgttacctg caattcagta
    35281 caaaacgtga tcagcccctc aattttccct tgctgaaaaa ttttccattg tctcccctgt
    35341 aaagctgtgc ttgtataaat attgttaaac acaaaaccaa caaggtcccc aatgactact
    35401 tccatgttca acgcaaaact actaccaact gcgctatccg ccgcagtggt cgtcgtgcgt
    35461 gtggtggtgg tcgtcggcaa tgcgccgtag ggactggaac aacacacgat tccaaaaccc
    35521 cgccggcgca aaccgggcgg ggtttttcgt ttaagcacct cccggaaagt cggcccagaa
    35581 gaaaaggact ggagcatggc aagttcgggc acaacatcga cgcgtaagcg ctttaccggc
    35641 gcagaattta tcgttcattt cctggaacag cagggcatta agattgtgac gggcattccg
    35701 ggcggttcta tcctgcctgt ttacgatgcc ttaagccaaa gcacgcaaat ccgccatatt
    35761 ctggcccgtc atgaacaggg cgcgggattt atcgctcagg ggatggcgcg caccgacggt
    35821 aaaccggcgg tctgtatggc ctgtagcgga ccgggtgcga ctaacctggt gaccgccatt
    35881 gccgatgcgc ggctggactc catcccgctg atttgcatca ccggtcaggt tcccgcctcg
    35941 atgatcggca ccgacgcctt ccaggaagtg gacacctacg gcatctctat ccccatcacc
    36001 aaacacaact atctggtcag acatatcgaa gaactcccgc aggtcatgag cgatgccttc
    36061 cgcattgcgc aatcaggccg cccaggcccg gtgtggatag acattcctaa ggatgtgcaa
    36121 acggcagttt ttgagattga aacacagccc gctatggcag aaaaagccgc cgcccccgcc
    36181 tttagcgaag aaagcattcg tgacgcagcg gcgatgatta acgctgccaa acgcccggtg
    36241 ctttatctgg gcggcggtgt gatcaatgcg cccgcacggg tgcgtgaact ggcggagaaa
    36301 gcgcaactgc ctaccaccat gactttaatg gcgctgggca tgttgccaaa agcgcatccg
    36361 ttgtcgctgg gtatgctggg gatgcacggc gtgcgcagca ccaactatat tttgcaggag
    36421 gcggatttgt tgatagtgct cggtgcgcgt tttgatgacc gggcgattgg caaaaccgag
    36481 cagttctgtc cgaatgccaa aattattcat gtcgatatcg atcgcgcaga gctgggtaaa
    36541 atcaagcagc cgcatgtggc gattcaggcg gatgttgatg acgtgctggc gcaattgatc
    36601 ccgcaggtgg aagcgcaacc gcgtgcagag tggcaccagt tggtagcgga tttgcagcgc
    36661 gaattcccgt gtccaatccc gaaagcgtgc gatccattaa gccattacgg cctgatcaac
    36721 gccgttgccg cctgtgtcga tgacaatgcg attatcacca ccgatgtggg tcagcatcag
    36781 atgtggaccg cgcaagctta tccgctcaat cgcccacgcc agtggctgac ctccggtggg
    36841 ctgggcacga tgggttttgg cctgcctgcg gcgattggcg cggcgctggc gaacccggat
    36901 cgcaaagtgt tgtgtttctc cggcgacggc agcctgatga tgaatattca ggagatggcg
    36961 accgccagtg aaaatcagct ggatgtcaaa atcattctga tgaacaacga agcgctgggg
    37021 ctggtgcatc agcaacagag tctgttctac gagcaaggcg tttttgccgc cacctatccg
    37081 ggaaaaatca actttatgca gattgccgcc ggattcggcc tcgaaacctg tgatttgaat
    37141 aacgaagccg atccgcaggc tgcattgcag gaaatcatca atcgccctgg cccggcgctg
    37201 atccatgtgc gcattgatgc cgaagaaaaa gtttacccga tggtgccgcc aggtgcggcg
    37261 aatactgaaa tggtggggga ataagccatg caaaacacaa ctcatgacaa cgtgattctg
    37321 gagctcaccg ttcgcaacca tccgggcgta atgacccacg tttgtggcct ttttgcccgc
    37381 cgcgctttta acgttgaagg cattctttgt ctgccgattc aggacagcga caaaagccat
    37441 atctggctac tggtcaatga cgaccagcgt ctggagcaga tgataagcca aatcgataag
    37501 ctggaagatg tggtgaaagt gcagcgtaat cagtccgatc cgactatgtt taacaagatt
    37561 gcggtgtttt ttcagtaacc gctcaaggct tgaacaacat cgcgcttatc gttaaggtaa
    37621 gcgcgttttt ttttacccgc caggacaaga ccatgatcac cgttgccctt atagacgatc
    37681 acctcatcgt ccgctccggc tttgcgcagc tgctggggct ggaacctgat ttgcaggtag
    37741 ttgccgagtt tggttcgggg cgcgaggcgc tggcggggct gccggggcgc ggtgtgcagg
    37801 tgtgtatttg cgatatctcc atgcccgata tctccggtct ggaactgcta agccagctgc
    37861 cgaaaggtat ggcgacaata atgctctctg ttcatgacag tccggcgctg gttgagcagg
    37921 cgcttaacgc gggggcacgc ggctttctct ccaaacgctg tagccctgac gaactgattg
    37981 cggcggtgca tacggttgcc acgggcggct gttatctgac gccggatatt gccattaaac
    38041 tggcatccgg tcgtcaggac ccgctaacca aacgcgaacg gcaggtggcg gaaaaactgg
    38101 cgcaaggaat ggcggtgaaa gagattgctg ccgaactggg cttgtcgccg aaaacggtgc
    38161 acgtccatcg cgccaacctg atggaaaaac tgggtgtcag taacgacgta gagctggcgc
    38221 gccgcatgtt tgatggctgg tgatgaagac gttgttctcc cgcttaatta ccgttattgc
    38281 ctgctttttt atcttctctg ccgcatggtt ttgcctgtgg agtatcagcc tgcacctggt
    38341 tgagcgccct gatatggcgg tgctgttatt tccgtttggt ctgcgtctgg ggctaatgct
    38401 gcaatgcccg cgcggatact ggccggtgct gctgggcgcg gagtggctgc tgatttactg
    38461 gctaacgcag gcggtcggtt taacccattt tccgttattg atgatcggta gtttactgac
    38521 gttactgccc gtggcgctga tctcgcgcta tcgccatcag cgtgactggc gcaccttgct
    38581 gttacagggt gcggcgttaa cggcggcggc gttgttgcag tcgctgccct ggctttggca
    38641 cggcaaagag tcgtggaatg cgctgttgct gactttaact ggcggcctga cgctggcccc
    38701 gatatgtctg gtgttctggc actatctcgc caataacacc tggctgccgc tcggcccgtc
    38761 actggtttct cagccgatca actggcgcgg gcgacatctg gtctggtact tgctgctgtt
    38821 tgttatcagt ctctggctcc agttgggatt gccggacgaa ctgtcgcgct ttacgccatt
    38881 ttgtctggcg ctgccgatta tcgcgctggc ctggcactat ggttggcaag gggcgctgat
    38941 tgcgacgttg atgaacgcca tcgcgctgat cgccagtcaa acctggcgcg atcatccggt
    39001 ggatttattg ctctcgctgc tggtgcaaag tctgacaggg ttgttgcttg gcgctggcat
    39061 ccagcggttg cgtgaactta accagtcgct gcaaaaggaa ctggcgcgca atcagcatct
    39121 ggctgaacgg ttgctggaaa ccgaagagag cgtgcgccgt gatgtggcgc gtgagctgca
    39181 tgatgatatc ggtcagacca tcacagccat tcgtactcag gcgggcattg ttcagcggct
    39241 ggcggcagat aacgccagcg tgaagcagag cgggcagctc atcgaacaac tatcgctggg
    39301 cgtttacgac gcggtgcgcc gtttgttggg tcggttacgt ccgcgccagt tggatgatct
    39361 caccctggag caggccatcc gctcactgat gcgggaaatg gagctggaag ggcgcggtat
    39421 tgtcagccat ctcgaatggc gaatcgatga atcagcgtta agcgaaaacc agcgcgtgac
    39481 gctgtttcgt gtctgccagg aagggctgaa caacattgtg aaacatgctg atgccagcgc
    39541 ggtcaccctg caaggctggc agcaggatga acggttgatg ctggttattg aagacgatgg
    39601 cagcggtttg ccgccgggtt ccgggcaaca aggttttggc ctcaccggaa tgcgcgagcg
    39661 cgtaacggcg ctgggtggca cattacacat ttcctgtctg cacggcacgc gtgtcagcgt
    39721 ttctctacct caacgctatg tctaaggttt gatgatgttg ccgtttctga aagcgcctgc
    39781 cgatgcgcca ttaatgactg ataaatatga aattgatgcc cgctatcgct actggcgtcg
    39841 gcatattctg ctgaccatct ggctgggtta cgcgctgttt tacttcacgc ggaaaagttt
    39901 taacgccgcg gtaccagaaa tccttgctaa cggcgtgctc agccgtagcg atatcggcct
    39961 gttagcgacc ctgttttaca ttacttacgg cgtgtcgaag tttgtctccg gcattgtcag
    40021 cgatcgctca aatgcccgtt attttatggg gatagggctt atcgccacgg gcatcatgaa
    40081 cattctgttt ggcttctcga cgtcgctatg ggcgtttgcc gtgctctggg tgctgaacgc
    40141 ctttttccag ggctggggtt caccggtgtg tgcgcgtctg ttaacggcct ggtattcacg
    40201 taccgagcgc ggcggttggt gggcattatg gaacacggcg cataacgtcg gcggcgcact
    40261 cattcccatt gtgatggcag cgagcgcgct gcattacggc tggcgtgccg ggatgatgat
    40321 tgctggttgt atggcgatag tcgtggggat ttttctctgc tggcggctac gcgatcgccc
    40381 gcaggcgtta ggtttaccgg cggtcggtga atggcggcac gacgcgctgg aaattgctca
    40441 acaacaagaa ggggcagggt tgacgcgtaa agagatcctg accaaatatg tgttgctgaa
    40501 tccgtatatc tggctgcttt cgttttgcta tgtgctggtc tatgtggtcc gggcggcaat
    40561 caacgactgg ggcaatttgt atatgtccga gacgctgggc gtcgatctgg tcacggcgaa
    40621 tacggcagtg acgatgtttg aactgggcgg atttatcggt gcgctggtag ccggttgggg
    40681 ctcggacaaa ttgtttaacg gcaaccgtgg gccgatgaat ttgattttcg ccgccggaat
    40741 tttgctttca gtcggctcgc tgtggctgat gccatttgcc agctacgtga tgcaggcaac
    40801 ctgcttcttc accattggtt tttttgtctt tggcccgcag atgttaatcg gcatggcggc
    40861 ggcagagtgt tcccacaaag aggctgcagg ggcggcgacg gggtttgtcg gcttgtttgc
    40921 ttatctgggg gcgtcgcttg ctggttggcc gctggcgaaa gtactcgata cctggcactg
    40981 gagcggattt tttgtggtta tctctatcgc cgccggggtt tccgcactgc tgttactgcc
    41041 ctttttgaac gcccaggcac cgcgcgaagc gtgatgcatc tcaccttttc acttcatatc
    41101 cggcaaaact aagaaatttt ctaggttttg cctggacgct atctcaggcc tgatttgctg
    41161 ctgattttta caatgccatc attcattaat tctttcccaa aataattcaa gttgcaggaa
    41221 cgcagcaaac gcgtgagtcc cgggagcata gtgaactatg tgaccgggag gaatgcgtgc
    41281 agctaaggca cctgcggctt gaagtatgaa gggaaatcag gagtaaccca tgctggcttt
    41341 cttaaaccag gttcgcaagc cgaccctgga ccttccgctc gaagtgcggc gcaaaatgtg
    41401 gttcaaaccg ttcatgcaat cctacctggt ggtctttatc ggctacctga cgatgtacct
    41461 gattcgcaag aactttaaca tcgcgcagaa cgacatgatc tcgacctacg ggttgagcat
    41521 gacgcagctg gggatgatcg gcctgggttt ctccatcact tatggcgtgg gtaaaacgct
    41581 ggtttcctac tacgccgacg gcaaaaacac caaacaattc ctgccgttca tgctgatcct
    41641 gtctgccatt tgtatgctgg gcttcagtgc cagtatgggc agcggctcgg ttagcctgtt
    41701 cctgatgatt gccttctacg ccttaagcgg ctttttccag agtaccggcg gttcgtgcag
    41761 ttactccacc atcaccaaat ggacgccgcg tcgtaaacgc gggacattcc tcggtttctg
    41821 gaatatttct cacaaccttg gtggtgcagg cgcagcaggt gtggcgctgt tcggggcaaa
    41881 ttacctgttc gatggccatg tcatcggcat gtttatcttc ccgtcgatta tcgcgctgat
    41941 tgtcggtttt atcggcctgc gttacggcag cgactccccg gaatcttatg gcctcggcaa
    42001 agctgaagaa ctgttcggcg aggagatcag cgaagaggac aaagagacag aatctaccga
    42061 tatgaccaag tggcagatct ttgttgagta tgtgctgaaa aacaaagtga tctggctgct
    42121 gtgcttcgcc aacattttcc tctatgtggt acgtattggt atcgaccagt ggtcaaccgt
    42181 atacgcgttc caggaactga aactctctaa agcggtggcg attcagggct ttacgctgtt
    42241 tgaagctggt gcactggtcg gtacgctgct gtggggctgg ctctctgacc tggcgaacgg
    42301 tcgccgtggc ctggtggcct gcatcgcgct ggcgctgatt atcgccacgc tcggtgtgta
    42361 tcaacatgcc agcaacgaat atatctatct ggcttctctc tttgcgttgg gtttcctggt
    42421 ctttggcccg caattgttga ttggtgtggc tgctgttggc tttgtaccta aaaaagcgat
    42481 tggcgctgcc gatggtatta aaggcacctt tgcttacctg attggtgaca gctttgccaa
    42541 gttaggtctg ggaatgattg ccgatgggac gccggtattc ggccttaccg gctgggcagg
    42601 caccttcgcc gcacttgata tcgccgcgat tggttgtatc tgcctgatgg cgatagtggc
    42661 ggtgatggaa gaacgcaaaa tccgccgcga gaaaaaaatt cagcagttga cagtggcata
    42721 aacgtaactg gtgacttttg cccggcatga cgccgggctt ttttattatt ccgtgacttc
    42781 cagcgtagtg aaggcaaact tctcgccatc aaatagcccc tgactggtta gttttagcgc
    42841 ggggatcact ggcagagaaa gaaacgccat ctgaataaac ggctcatcgg gtaacggacc
    42901 gcattcacgg gcggcggctt tcaaggcgtc aatttgttcc gccagcgact gcgccgtgtc
    42961 ggtgctcatc aggccggcaa tgggcaacgg cagatgactt tggacctggc cgttacgtac
    43021 cacgcacagc ccgccgccat cctgaatcac ctgattgacc gccagcgcca tctcttcggc
    43081 actgcgaccg atcaccacaa tattatggct gtcatggctg accgtcgcag ccagcgcacc
    43141 ttcattcagt ccaaagccgc caagcaaacc acaagccgga gccagccgtt gcccgtaacg
    43201 ctcaagtacg gcaataaagc tcacatcatc gcgatcaaaa ccattttcgc tgtagacgct
    43261 ggagtgggag tgcgtaatca attcgttatg gatgacgtca atgacccgat agcgttttcc
    43321 gggcgtaaat tgcagggcaa agtcgctggc ggaaactggc tggcgggcaa tggtgttgcc
    43381 atatggcgga gcggattgtg ccagtctcgc cgactcttcc gcctgtaagg tttgcgcatc
    43441 aatcggctcg cctttcacca gtacctgctg caccgtgacc ttacgcgcat cgctcaacag
    43501 gacgatatcg gcctgcttgc cgggtgccag taagccgagg tgattcagac caaagtggcg
    43561 cgccgtcgac cagctggcga cgcgatatgc cacatgcagc ggcacattgt gttgttcgat
    43621 caggcggcga attaaggcat cgatgtgtcc ttcatgggcg atctcccacg ggttacggtc
    43681 atcggtacag agcatgcatt gcgggctgtt aaattcgttg atcaacggtg ccagcgcgtt
    43741 gagattgcgg gcagcggacc cttcgcggat catcaacgac atgccgagtt gtaatttccg
    43801 gcgtccttct tccagctgat aactttcgtg gcagttttca atacccgcag taatataggc
    43861 gttaagttct ttaccaccca aacccgggca gtgaccgtcc agcgtcaggt ggcgaaatgc
    43921 atccagttta tcgagcagcg cattctgccc gctaattacg ccagggtagt ccatcatttc
    43981 tgcaaggccg gtaacctgcg gatggtcccg ccaggcgagc atcaatccgc tgcaaagccg
    44041 gagcatcagc gtattctgcg ccaacaccgg caatgtaacg tcctttaatc acaattgggc
    44101 cggaaatttc tccgccattg atcaggtcga gaatagagac attatcaata atataatcgg
    44161 caacagcgtc gccacgggaa acggctaaca attcctggta ttcagcccgg ctaatgtgat
//
LOCUS       DAZGTR010000025        41829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGTR010000025 DAZGTR010000000
VERSION     DAZGTR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.025"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            330..416
                     /gene="leuL"
                     /locus_tag="ACHLCY_001556"
     CDS             330..416
                     /gene="leuL"
                     /locus_tag="ACHLCY_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP0368847.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            509..2080
                     /gene="leuA"
                     /locus_tag="ACHLCY_001557"
     CDS             509..2080
                     /gene="leuA"
                     /locus_tag="ACHLCY_001557"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP0368848.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2080..3171
                     /gene="leuB"
                     /locus_tag="ACHLCY_001558"
     CDS             2080..3171
                     /gene="leuB"
                     /locus_tag="ACHLCY_001558"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP0368849.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3174..4574
                     /gene="leuC"
                     /locus_tag="ACHLCY_001559"
     CDS             3174..4574
                     /gene="leuC"
                     /locus_tag="ACHLCY_001559"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP0368850.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4585..5190
                     /gene="leuD"
                     /locus_tag="ACHLCY_001560"
     CDS             4585..5190
                     /gene="leuD"
                     /locus_tag="ACHLCY_001560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP0368851.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5239..6417)
                     /gene="setA"
                     /locus_tag="ACHLCY_001561"
     CDS             complement(5239..6417)
                     /gene="setA"
                     /locus_tag="ACHLCY_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFP0368852.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6520..6651)
                     /gene="sgrT"
                     /locus_tag="ACHLCY_001562"
     CDS             complement(6520..6651)
                     /gene="sgrT"
                     /locus_tag="ACHLCY_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP0368853.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6740..8395
                     /gene="sgrR"
                     /locus_tag="ACHLCY_001563"
     CDS             6740..8395
                     /gene="sgrR"
                     /locus_tag="ACHLCY_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP0368854.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8429..8528
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8559..9542
                     /gene="thiB"
                     /locus_tag="ACHLCY_001564"
     CDS             8559..9542
                     /gene="thiB"
                     /locus_tag="ACHLCY_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP0368855.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9518..11128
                     /gene="thiP"
                     /locus_tag="ACHLCY_001565"
     CDS             9518..11128
                     /gene="thiP"
                     /locus_tag="ACHLCY_001565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP0368856.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11112..11810
                     /gene="thiQ"
                     /locus_tag="ACHLCY_001566"
     CDS             11112..11810
                     /gene="thiQ"
                     /locus_tag="ACHLCY_001566"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP0368857.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11924..12688)
                     /gene="yabI"
                     /locus_tag="ACHLCY_001567"
     CDS             complement(11924..12688)
                     /gene="yabI"
                     /locus_tag="ACHLCY_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0368858.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12774..13652)
                     /gene="araC"
                     /locus_tag="ACHLCY_001568"
     CDS             complement(12774..13652)
                     /gene="araC"
                     /locus_tag="ACHLCY_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP0368859.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRYPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13991..15691
                     /gene="araB"
                     /locus_tag="ACHLCY_001569"
     CDS             13991..15691
                     /gene="araB"
                     /locus_tag="ACHLCY_001569"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP0368860.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15702..17204
                     /gene="araA"
                     /locus_tag="ACHLCY_001570"
     CDS             15702..17204
                     /gene="araA"
                     /locus_tag="ACHLCY_001570"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP0368861.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDNPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17373..18068
                     /gene="araD"
                     /locus_tag="ACHLCY_001571"
     CDS             17373..18068
                     /gene="araD"
                     /locus_tag="ACHLCY_001571"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0368862.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18143..20494
                     /gene="polB"
                     /locus_tag="ACHLCY_001572"
     CDS             18143..20494
                     /gene="polB"
                     /locus_tag="ACHLCY_001572"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP0368863.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPPLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20659..23565
                     /gene="rapA"
                     /locus_tag="ACHLCY_001573"
     CDS             20659..23565
                     /gene="rapA"
                     /locus_tag="ACHLCY_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP0368864.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALVREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23577..24236
                     /gene="rluA"
                     /locus_tag="ACHLCY_001574"
     CDS             23577..24236
                     /gene="rluA"
                     /locus_tag="ACHLCY_001574"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP0368865.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYVADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24353..25168)
                     /gene="djlA"
                     /locus_tag="ACHLCY_001575"
     CDS             complement(24353..25168)
                     /gene="djlA"
                     /locus_tag="ACHLCY_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP0368866.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25423..27777
                     /gene="lptD"
                     /locus_tag="ACHLCY_001576"
     CDS             25423..27777
                     /gene="lptD"
                     /locus_tag="ACHLCY_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP0368867.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYRNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27830..29116
                     /gene="surA"
                     /locus_tag="ACHLCY_001577"
     CDS             27830..29116
                     /gene="surA"
                     /locus_tag="ACHLCY_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP0368868.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29116..30105
                     /gene="pdxA"
                     /locus_tag="ACHLCY_001578"
     CDS             29116..30105
                     /gene="pdxA"
                     /locus_tag="ACHLCY_001578"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP0368869.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRSYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30102..30923
                     /gene="rsmA"
                     /locus_tag="ACHLCY_001579"
     CDS             30102..30923
                     /gene="rsmA"
                     /locus_tag="ACHLCY_001579"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP0368870.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30926..31303
                     /gene="apaG"
                     /locus_tag="ACHLCY_001580"
     CDS             30926..31303
                     /gene="apaG"
                     /locus_tag="ACHLCY_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP0368871.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRTPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31310..32158
                     /gene="apaH"
                     /locus_tag="ACHLCY_001581"
     CDS             31310..32158
                     /gene="apaH"
                     /locus_tag="ACHLCY_001581"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP0368872.1"
                     /translation="MATYLIGDVHGCYDELFALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32183..32782
                     /locus_tag="ACHLCY_001582"
     CDS             32183..32782
                     /locus_tag="ACHLCY_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFP0368873.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32818..33285
                     /locus_tag="ACHLCY_001583"
     CDS             32818..33285
                     /locus_tag="ACHLCY_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368874.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33384..33563)
                     /locus_tag="ACHLCY_001584"
     CDS             complement(33384..33563)
                     /locus_tag="ACHLCY_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP0368875.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33584..34063)
                     /gene="folA"
                     /locus_tag="ACHLCY_001585"
     CDS             complement(33584..34063)
                     /gene="folA"
                     /locus_tag="ACHLCY_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP0368876.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34255..36117)
                     /gene="kefC"
                     /locus_tag="ACHLCY_001586"
     CDS             complement(34255..36117)
                     /gene="kefC"
                     /locus_tag="ACHLCY_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP0368877.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36110..36640)
                     /gene="kefF"
                     /locus_tag="ACHLCY_001587"
     CDS             complement(36110..36640)
                     /gene="kefF"
                     /locus_tag="ACHLCY_001587"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP0368878.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36748..38079)
                     /gene="yaaU"
                     /locus_tag="ACHLCY_001588"
     CDS             complement(36748..38079)
                     /gene="yaaU"
                     /locus_tag="ACHLCY_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0368879.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38138..38425)
                     /gene="fixX"
                     /locus_tag="ACHLCY_001589"
     CDS             complement(38138..38425)
                     /gene="fixX"
                     /locus_tag="ACHLCY_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP0368880.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38422..39708)
                     /gene="fixC"
                     /locus_tag="ACHLCY_001590"
     CDS             complement(38422..39708)
                     /gene="fixC"
                     /locus_tag="ACHLCY_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0368881.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39759..40700)
                     /gene="fixB"
                     /locus_tag="ACHLCY_001591"
     CDS             complement(39759..40700)
                     /gene="fixB"
                     /locus_tag="ACHLCY_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0368882.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40715..41485)
                     /gene="fixA"
                     /locus_tag="ACHLCY_001592"
     CDS             complement(40715..41485)
                     /gene="fixA"
                     /locus_tag="ACHLCY_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP0368883.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10187 a        11445 c        10880 g         9317 t
ORIGIN      
        1 caataatatt caccaaatca atatcaaaaa aatcgcaaaa catataattc aatacaaatc
       61 atcaggatag gttttgcaac gcgtgcattt tgtccccttt ttcctcgttg attagatgca
      121 aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg gtagttaagc agaaattaat
      181 atcgcttact ttaaccaccg cagcacaatt agctaatttt acgggtgcag aactcacgct
      241 ggcgggacgt ttttattgcg tcagggttga catccgtttt tgtatccagt aactctaaaa
      301 gcatatcgta ttcatctgga gctgatttaa tgactcacat cgttcgcttt atcggtctac
      361 tactactaaa cgcatcttct ttgcgcggta gacgagtgag cggcatccag cattaagcca
      421 gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt tttttatgcc cgaagcgagg
      481 cgctctaaaa gagacaagga cccaaaccat gagccagcaa gtcattattt tcgataccac
      541 attgcgcgac ggtgaacagg cgttacaggc aagcttgagt gtgaaagaaa aactgcaaat
      601 tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa gtcggtttcc ccgtctcttc
      661 gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag gttaaaaaca gccgcgtatg
      721 tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg gccgaatccc tgaaagtcgc
      781 cgaagccttc cgtattcata cctttattgc tacctcccca atgcacatcg ccaccaagct
      841 gcgcagcacc ctggacgagg tgatcgaacg cgctatctat atggtgaaac gcgcccgtaa
      901 ttacaccgat gatgttgaat tttcttgcga agatgccggg cgtacaccca ttgccgatct
      961 ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc accatcaaca ttccggacac
     1021 cgtgggctac accatgccgt ttgagttcgc cggaatcatc agcggcctgt atgaacgcgt
     1081 acctaacatc gacaaagcca ttatttccgt acatacccac gacgatttag gcctggcggt
     1141 cggcaactca ctggcggcgg tacatgccgg tgcacgccag gtggaaggtg caatgaacgg
     1201 gatcggcgag cgtgccggta actgttcgct ggaagaagtc atcatggcga ttaaagttcg
     1261 taaggatatt ctcaacgtcc acaccgccat taatcaccag gaaatatggc gcaccagcca
     1321 gttagttagc cagatttgta atatgccgat cccggcaaac aaagccattg ttggcagcgg
     1381 cgcattcgca cactcctccg gtatccacca ggatggcgtg ctgaaaaacc gcgaaaacta
     1441 cgaaatcatg acaccagaat ctattggtct gaaccaaatc cagctgaatc tgacctctcg
     1501 ttcggggcgt gcggcggtga aacatcgcat ggacgagatg gggtataaag aaagtgaata
     1561 taatttagac aatttgtacg acgccttcct gaagctggcg gacaaaaaag gtcaggtgtt
     1621 tgattacgat ctggaggcgc tggccttcat cggtaagcag caagaagagc cggagcattt
     1681 ccgtctggat tacttcagcg tgcagtctgg ctctaacgat atcgccaccg ccgccgtcaa
     1741 actggcctgt ggcgaagaag tcaaagcaga agccgccaac ggtaacggtc cggtcgatgc
     1801 cgtctatcag gcaattaacc gcatcactga ctataacgtc gaactggtga aatacagcct
     1861 gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg gatattgtcg ccaactacaa
     1921 cggtcgccgc ttccacggcg tcggcctggc caccgatatt gtcgagtcct ccgccaaagc
     1981 catggtgcac gtactgaaca atatctggcg tgccgcagaa gtcgaaaaag agttgcaacg
     2041 caaagctcaa cacaacgaaa acaacaagga aaccgtgtga tgtcgaagaa ttaccatatt
     2101 gccgtattgc cgggggacgg tattggtccg gaagtgatga cccaggcgct gaaagtgctg
     2161 gatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca gccattacga tgtaggcggt
     2221 gcagccattg ataaccacgg gcaaccactg ccgcctgcga cggttgaagg ttgtgagcaa
     2281 gccgatgccg tgctgtttgg ctcggtaggc ggtccgaaat gggaacattt accaccagac
     2341 cagcaaccag aacgcggcgc gctgttgcct ttgcgtaagc acttcaaatt attcagcaac
     2401 ctgcgcccgg caaaactgta tcaggggctg gaagcattct gtccgctgcg tgcagacatt
     2461 gccgctaacg gcttcgacat cctgtgcgtg cgcgaactga ccggcggcat ctatttcggt
     2521 cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag cgtttgatac cgaggtgtat
     2581 caccgttttg agatcgagcg tattgcccgc atcgcgtttg aatctgctcg taaacgtcgc
     2641 cacaaagtca cctcaatcga caaagctaac gtgctgcaat cctctatttt atggcgtgaa
     2701 atcgtcaacg agatcgccac ggaatacccg gatgtcgaac tggcgcatat gtacatcgac
     2761 aacgccacca tgcagctgat taaagatcca tcacagtttg acgttctgct gtgctccaac
     2821 ctgtttggcg acattctgtc tgacgagtgc gcaatgatca ctggctcgat ggggatgttg
     2881 ccttccgcca gcctgaacga gcaaggtttt ggtctgtatg aaccggcagg cggctcagca
     2941 ccagatatcg caggcaaaaa catcgccaac ccgattgcgc aaattctgtc gctggcactg
     3001 ctgctgcgct acagcctgga tgccgatgat gcggcttgcg ccattgaacg cgccattaac
     3061 cgcgcattag aagaaggcat tcgcaccggg gatttagccc gtggcgctgc cgccgttagt
     3121 accgatgaaa tgggcgatat cattgcccgc tatgtggcag aaggggtgta atcatggcta
     3181 aaacgttata cgaaaaatta ttcgacgctc acgtagtgta cgaagccgaa aacgaaaccc
     3241 cgctgttata tatcgaccgc cacctggtgc atgaagtgac ctcaccgcag gcgttcgatg
     3301 gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa aaccttcgcc actatggatc
     3361 acaacgtctc tacccagacg aaagacatta atgcctgtgg tgaaatggcg cgcatccaga
     3421 tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga actgtatgac ctgaatcacc
     3481 cgtatcaggg gatcgtccac gtaatggggc cggaacaggg cgtcaccttg ccggggatga
     3541 ccattgtctg cggcgactcg cataccgcca cccacggcgc gtttggcgca ctggcctttg
     3601 gtatcggcac ttccgaagtt gaacacgtac tggcaacgca aaccctgaaa cagggccgcg
     3661 caaaaaccat gaaaattgaa gtccagggca aagccgcgcc gggcattacc gcaaaagata
     3721 tcgtgctggc aattatcggt aaaaccggta gcgcaggcgg caccgggcat gtggtggagt
     3781 tttgcggcga agcaatccgt gatttaagca tggaaggtcg tatgaccctg tgcaatatgg
     3841 caatcgaaat gggcgcaaaa gccggtctgg ttgcaccgga cgaaaccacc tttaactatg
     3901 tcaaaggccg tttgcatgcg ccgaaaggca aagatttcga cgacgccgtt gcctactgga
     3961 aaaccctgca aactgacgaa ggcgcaactt tcgataccgt tgtcactctg caagcagaag
     4021 aaatttcacc gcaggtcacc tggggtacca atccaggcca ggtgatttcc gtgaacgaca
     4081 atattcccga tccggcttcg tttgccgatc cggttgaacg cgcgtcggca gaaaaagcgc
     4141 tggcctatat ggggctgaaa ccgggcattc cgctgaccga agtggctatc gacaaagtgt
     4201 ttatcggttc ctgtaccaac tcgcgcattg aagatttacg cgcggcagcg gagatcgcca
     4261 aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt tcccggctct ggaccggtaa
     4321 aagcccaggc ggaagcggaa ggtctggata aaatctttat tgaagccggt tttgaatggc
     4381 gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga ccgtctgaat ccgggcgaac
     4441 gttgtgcatc caccagcaac cgtaactttg aaggccgcca ggggcgcggc gggcgcacgc
     4501 atctggtcag cccggcaatg gctgccgctg ctgctgtgac cggacatttc gccgacattc
     4561 gcaacattaa ataaggagca caccatggca gagaaattta tcaaacacac aggcctggtg
     4621 gttccgctgg atgccgccaa tgtcgatacc gatgcaatca tcccgaaaca gtttttgcag
     4681 aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg actggcgttt tctcgatgaa
     4741 aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc cgcagtatca gggcgcttcc
     4801 attttgctgg cacgagaaaa cttcggctgt ggctcctcgc gtgagcacgc gccctgggca
     4861 ttgaccgact acggttttaa agtggtgatt gcgccgagtt ttgctgacat cttctacggc
     4921 aatagcttta acaaccagct gctgccggtg aaattaagcg atgcagaagt ggacgaactg
     4981 tttgcgctgg tgaaagccaa tccggggatc catttcgacg tggatctgga agcgcaagag
     5041 gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg ccttccgccg ccactgcatg
     5101 atgaacggtc tggacagtat tgggcttacc ttgcagcacg acgacgccat tgccgcttat
     5161 gaagcaaaac aacctgcgtt tatgaattaa tccccttgcc cggtcaaatg accgggcttt
     5221 ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg gttaaaaata atgcgacaac
     5281 agaaataacc gcaattaccc agtagacagc aaagtgcccc caactttgtg caattgctcc
     5341 ctgaataacg ccagccagaa ttaccccggt agaaatactg ttagtaaata aggtggtagc
     5401 tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat
     5461 gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa
     5521 aatcaatccg gtgtaaaaca gtactcctgc cgccactgct atgaccatca ttcgccgctt
     5581 accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc
     5641 cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aactcgctac tgatccacaa
     5701 cggcatatca ataatgtaca tggtgttgca ggtccacatt aacgtcgagg cgacaaataa
     5761 catccgtacg ttactatcct gccagccacc ttgcattgat aaagcatttt ccgacggcag
     5821 ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat
     5881 cccggcggca atcgaaaaca tcaccgtaaa gccgtaattc aacgccagca taaaggccaa
     5941 cggtggaccg ataacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac
     6001 cacttctcgc gccgagttat ccgcatattc ccgcgccaga gcaaataact gtggcattgc
     6061 cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt
     6121 aaatgcaaac aatagcgcat tgccgatagc catcaaacag caaaatataa tcagttttcg
     6181 ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat
     6241 agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag
     6301 aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag
     6361 cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg
     6421 ttcccttttt agcgcggcga gaataaaaaa aaaccagcag gtataatctg ctggcgggtg
     6481 attttacacc aatactcagt cacacatgat gcaggcaagt caactttcag aattgcggtc
     6541 atcccactgc atcagttctt caagcatggt taatcgttgt gggacgctta accaacgcaa
     6601 ccagcacaac ttcgctgtcg cggtaaaata gtgctgataa aactgacgca tggggcacct
     6661 ccttgcttca tcgttaggaa ttattgactt aatataggga aaataaaatt gctgtctttt
     6721 gcgcaggagt tcccctttta tgccatctgc tcgtctgcaa caacagttca tccgcctgtg
     6781 gcaatgctgc gagggtaaat cgcaggacac aacgctcaac gaactggcag cgttattgag
     6841 ctgctcgcgt cgtcatatgc gtaccctgct caacaccatg caggatcgcg gctggctgac
     6901 gtgggaagcg gaagtcgggc gcggtaaacg ctcgcgtctg acattcctct ataccgggct
     6961 ggcgcttcag caacagcggg cggaagacct gctggagcag gatcgtatcg accaactagt
     7021 gcagttggtt ggcgacaaag cgactgtgcg gcaaatgctg gtttctcatc tgggccgcag
     7081 cttccgccag gggcggcaca tcctgcgcgt gctctactat cgtccgttgc gtaatctgct
     7141 acctggcagc gcattgcgcc gttccgaaac ccatattgcc cggcaaatct tcagttcgct
     7201 aacgcgcata aatgaggaaa atggggaact ggaagcagac atcgcccacc actggcagca
     7261 aatatcaccg cttcactggc gtttcttttt gcgtccagga gtccattttc atcatggtcg
     7321 tgaactggaa atggacgacg tgatcgcctc tttaaaacga atcaatacgc tgccgctcta
     7381 ttcgcatatt gcggacattg tgtcgccgac gccctggacg ctggatatcc atctcacgca
     7441 accggaccgc tggttaccgt tactgctggg acaagttccg gcgatgatcc tgccgcgcga
     7501 atgggaaacc ctcagtaact ttgccagcca tcccatcggc accggtccgt atgcggtgat
     7561 ccgcaacacc accaatcaac tgaaaattca ggcattcgat gacttcttcg gttaccgggc
     7621 attaatcgat gaagttaacg tctgggttct gccggaaatt gccgacgagc cagctggagg
     7681 tctgatgcta aaaggtccac agggtgagga aaaagagatt gaaagccgcc tggaggaagg
     7741 ttgctactat ttattgttcg acagccgcac ccatcgcggg gcgaatcagc aagtcaggga
     7801 ctgggtaagc tatgtgcttt ctccaactaa tctggtctat ttcgctgagg aacagtacca
     7861 gcaactgtgg ttcccggctt atggactgct cccccgttgg caccatgccc gcaccataaa
     7921 gagcgaaaaa ccggctggcc tggaaagcct catcctgacc ttttatcagg atcacagtga
     7981 gcatcgggtg attgccggga tcatgcagca gattctggca agtcaccagg tcacgctgga
     8041 aatcaaagag atcagctacg atcagtggca tgaaggagag atcgagagcg atatctggct
     8101 taacagcgcc aactttacgc tgccgctgga tttttcgctg ttcgcgcact tgtgcgaggt
     8161 gccgctgctc caacactgta ttccgatcga ctggcaagcc gacgccgccc gctggcgcaa
     8221 tggcgaaatg aacctggcga actggtgcca gcaactggtc gccagcaaag caatggtgcc
     8281 gcttatccac cactggctga tcattcaggg gcaacgcagt atgcgcggcc tgcgcatgaa
     8341 caccctcggc tggttcgatt ttaaatcagc gtggtttgcg ccaccggatc catgattgct
     8401 ggtagataac aaaatcacta cactaacgcc gttctcaacg gggtgccacg caaacgcgtg
     8461 cgctgagaaa atacccgtcg aacctgatcc ggataacgcc ggcgaaggga tttgaggctc
     8521 cttctcaagt cctttgccac tcttttttga ggtgcaaagt gttaaaaaaa tgtcttcccc
     8581 tgctgttgct gtgcacagcg cccgttttcg ctaaacccgt tctgactgtt tatacctacg
     8641 attccttcgc cgccgactgg gggcctggtc caaagatcaa aaaagctttt gaagccgact
     8701 gtaactgcga actgaaactg gtggcactgg aagatggcgt ttcgcttctc aaccgtctac
     8761 ggatggaagg caaaaatagt aaagccgatg tggtgctggg gctggataac aatctgttag
     8821 acgccgccag taaaaccgga ctatttgcca aaagcggtgt ggcagcggat gccgttaacg
     8881 ttcccggcgg ctggaataat gacactttcg tcccgtttga ttacggctat ttcgccttcg
     8941 tctatgacaa gaacaaactg aaaaacccgc cgcaaagcct gaaagaattg gttgagagcg
     9001 atcagaactg gcgggtgatt tatcaggatc cgcgcaccag tacgccgggg ctgggcctgc
     9061 tgttgtggat gcaaaaagtc tatggcgatg acgccccaca agcctggcaa aaactggcga
     9121 agaaaaccgt cacggtcacc aaaggctgga gcgaagccta cggcctgttt ttaaaaggtg
     9181 aaagcgatct ggtactgagt tacaccacct ctccggctta tcacattctc gaagagaaaa
     9241 aagataacta cgccgccgcg aactttagcg aaggtcacta tctgcaggtg gaagtcgccg
     9301 cccgcaccgc tgccagcaag cagccggagc tggcgcaaaa attcctccag tttatggttt
     9361 ctccggcttt tcagaatgcg atcccaaccg gtaactggat gtatccggtg gcaaatgtca
     9421 cgctgcctgc cgggtttgaa caattgacca aaccggccac cacgctggag ttcacaccag
     9481 ctgaagtggc tgcacaacgt caggcatgga ttagcgaatg gcaacgcgcc gtcagccgtt
     9541 aattcccggc tggttaattc caggtgtaag cgccgccacg ctggtggtag cggttgcgct
     9601 cgcggcgttt ctcgccctgt ggtggaacgc gccgcagggt aactgggtgg cagtctggca
     9661 ggacagctac ctgtggcatg tggtgcgctt ctccttctgg caggcgtttc tctcggcgct
     9721 actctctgtc gtacccgcga tattcctcgc ccgcgccctc tatcgcaggc gctttccggg
     9781 tcggctggcg ctgttgcgtc tgtgcgcaat gaccttgatc ctcccggtgc tagttgctgt
     9841 tttcggcatt cttagcgtct atggtcgtca gggctggctg gcatcgctct gccaatcgct
     9901 cggtctggag tggacctttt cgccctacgg cctgcaaggc attttgctgg cgcacgtatt
     9961 ttttaatctg ccgatggcga gccgcttatt actccaggca ctggaaaaca ttcccggcga
    10021 acaacgtcag cttgccgccc agcttgggat gcgcggctgg cattttttcc gcttcgtcga
    10081 atggccgtgg ctacggcgac aaatcccgcc ggttgccgcg cttatcttta tgctctgttt
    10141 cgccagcttc gccaccgtgc tatcgctggg cggcggtccg caggcaacta ctatcgagct
    10201 ggctatttat caggcgctga gttacgacta cgatcctgcc cgcgcggcga tgctggcgct
    10261 gctccagatg gtgtgttgcc ttgggctggt gctgttgagt cagcgactaa gtaaggccat
    10321 tgcgcccggt accacgctgc tgcaaggctg gcgcgatccg gacgatcgtc tgcatagccg
    10381 catttgcgac acggtgttaa tagtgctggc gctgctgctg ttgctgccac cgttgctggc
    10441 ggtgatcgtc gatggggtaa atcgccagtt gccggaagtg ctggcacaac cggtgctgtg
    10501 gcaggcgctg tggacctcgt tgcgtattgc gctggcggca ggtgtattgt gcgtagtgct
    10561 gaccatgatg ctgctatgga gcagtcgcga actgcgggcg cggcagaaaa tgctggcggg
    10621 tcaggcgctg gagatgagcg gcatgttgat cctcgccatg ccggggattg tgctggctac
    10681 cggcttcttt ttactgctca acaacactat cggcctgcca caatctgctg acggcattgt
    10741 gattttcacc aatgcgttaa tggcgatccc ttatgcgctg aaagtactgg aaaacccgat
    10801 gcgcgatatc accgcccgct acagtatgtt gtgtcagtcg ctgggcattg aaggctggtc
    10861 gcgcttaaaa gtggtcgagc tgcgcgccct gaaacgtcca ctggcgcagg cgctggcttt
    10921 tgcctgcgtg ctgtcgattg gtgattttgg cgtagtggcg ttgttcggta acgatgattt
    10981 ccgtaccctg ccgttttatc tctaccagca aattggctcc tatcgcagcc aggacggcgc
    11041 ggtcaccgcg ttaattctgc tactactctg ttttctgctg tttaccgtga ttgaaaaact
    11101 accggggcga aatgttaaaa ctgactgata tcacctggct ttatcaccat ttgccgatgc
    11161 gttttagctt aacggtagaa cgcggcgagc aggtggcaat cctcgggcca agcggcgcgg
    11221 gtaaaagtac cctgctgaat ttgattgccg gttttctgac gccagccagc ggttcgctga
    11281 ctatcgacag cgttgatcac accacgacgc cgccgtcacg ccgcccggta tcgatgctgt
    11341 ttcaggagaa caacctgttc agccacctga cggtcgcgca gaacatcggg ctggggctta
    11401 atccgggatt gaagctgaac gcggcgcagc aggagaaaat gcacgctatc gcccgccaga
    11461 tggggattga taatttaatg gcgcggttac cgggcgagct ttccggcggt cagcgacagc
    11521 gcgtggcgtt agcgcgttgt ctggtgcgcg aacagccgat tttattgctc gatgaaccgt
    11581 tctctgcgct cgatccggcg ttacgtcagg agatgctgac gctggtgagc acgagctgcc
    11641 agcagcaaaa aatgacgcta ttgatggtgt cgcacagtgt ggaagatgcg gcgcggatcg
    11701 ccacacgctc ggtggtggtc gccgacgggc gcatcgcctg gcagggtaag accaatgagt
    11761 tgttgagcgg taaggcgagt gcttcggcac tattggggat tacgggttag tatgcactgt
    11821 cggatgcggc gtgaacgcct tatccgacct acgggtagtg cgttttgtag gcctgataag
    11881 acgcggtcag cgtcgcatca ggcaacccgc acaagactat ctcctaaccc ccaaccactt
    11941 tacgcaaaat atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca
    12001 ccacgccgat ggcagaaatt aacggcgtca gccacaacaa acggccgcgc gacaaataat
    12061 gacttaaacg atcggttgct ttaccgctgc gccataaccg ccagcacagc cagccaccaa
    12121 cccacaaaaa taccgccgtc gccagcagca accatttaaa ctcgccgctc tgcattccgg
    12181 ctggaatatc gatcgccgcg cccgccagaa tcccaggcag gaagtaaaac ggtggccaca
    12241 gcaggcagcc gataatattc ggcgtaatga attttgccac cggcagatcc agcattcccg
    12301 ccaccattgg caccagcgga cgcgtcgggc caacaaagcg accgaccaga atggtgaaca
    12361 tgctgtgttg atgcaacgca tgttcagttt tatcgagtaa tgctttgttt ttcttcagaa
    12421 atgaccagcg atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc
    12481 ccagcaggca accgacgatc cctgccagcc aggcgtgcca gaaacttaac tcgccgctgc
    12541 caatcagcgc tcccagccct gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg
    12601 ccagcgactc cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtagattggg
    12661 taataaagtg ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt
    12721 ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac
    12781 aacttgacgg ctacatcatt cactttttct tcacaaccgg ctcggaactc gctcgggctg
    12841 gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg
    12901 cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata
    12961 cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac
    13021 agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct
    13081 gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg
    13141 agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc
    13201 agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg
    13261 ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac
    13321 ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac
    13381 cattcgcgag cctccggata acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa
    13441 atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga
    13501 atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga
    13561 taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc
    13621 ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa
    13681 gaaaccaatt gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc
    13741 tcgctaaccc aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa
    13801 gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat
    13861 tatttgcacg gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat
    13921 cctacctgac gctttttatc gcaactctct actgtttctc catacccgtt tttttggatg
    13981 gagtgaaacg atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt
    14041 ggcggtggac tgcgccagcg gtgaagagat cgccaccagc gtagagtggt atccccgttg
    14101 gcaaaaaggg caattttgtg atgccccgaa taaccagttc cgtcatcatc cgcgtgacta
    14161 cattgagtca atggaagcgg cactgaaaac cgtgcttgca gagcttagcg tcgaacagcg
    14221 cgcagctgtg gtcgggattg gcgttgacag taccggctcg acgcccgcac cgattgatgc
    14281 cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa aacccgaacg cgatgttcgt
    14341 attgtggaaa gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc
    14401 gccgggcaac gttgactact cccgctacat tggcggtatt tattccagcg aatggttctg
    14461 ggcaaaaatc ctgcatgtga ctcgccagga caccgccgtg gcgcaatctg ccgcatcgtg
    14521 gattgagctg tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat
    14581 tcgtcgcgga cgttgcagcg ccgggcataa gtcgctatgg cacgaaagct ggggtggctt
    14641 gccgccagcc agtttctttg atgagctgga cccgatcctc aatcgccatt taccttcccc
    14701 gctgttcact gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc
    14761 gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat
    14821 gggcgcagtt ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac
    14881 ctgcgacatt ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg
    14941 cggtcaggtt gatggcagcg tggtacctgg atttatcggt ctggaagcag gccaatcggc
    15001 gtttggtgat atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc
    15061 cgcccagcat ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc
    15121 gctgaccgaa gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg
    15181 gtttaacggt cgtcgcacac cgaacgctaa ccaacgcctg aaaggggtga ttaccgatct
    15241 taacctcgct accgacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg
    15301 cgcacgcgca atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc
    15361 gctgggcggc atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa
    15421 tcgcccgctg caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc
    15481 tgccgtcgcc gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc
    15541 ggtagagaaa accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg
    15601 ccgctatcag caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc
    15661 acaggctgcc caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt
    15721 atgaagtgtg gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg
    15781 tcacccaaca tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca
    15841 aactagtgtt gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg
    15901 cgaattacga cgatcgttgc gctggtctgg tggtgtggct gcacactttc tccccggcca
    15961 aaatgtggat caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt
    16021 ttaacgcggc gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg
    16081 cacatggcgg tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg
    16141 ttaccggtca ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg
    16201 cggtctctaa acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg
    16261 aagtagcggt caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca
    16321 atacctgggc ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta
    16381 acgcgctggt cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg
    16441 gcgaaaaacg acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc
    16501 tggaacaagg tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac
    16561 agcttccagg tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag
    16621 gcgactggaa aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg
    16681 gcggcacctc ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc
    16741 tcggctccca tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg
    16801 acgttcagca tctcggtatt ggtggtaagg acaatcctgc ccgactgatc ttcaataccc
    16861 aaaccggtcc agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg
    16921 ttaactgtat cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg
    16981 cgctgtggaa agcgcaaccg gatctgccaa ctgcttccga agcgtggatc cttgctggtg
    17041 gcgcgcacca taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg
    17101 agatgcacga cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag
    17161 acgcactgcg ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta
    17221 tgtaacgcct gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg
    17281 ccggataagc aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg
    17341 gggtgagggg aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat
    17401 tagaagccaa cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca
    17461 gcgccgttga tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg
    17521 tcatgaccgc tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta
    17581 cgaaaaagcc ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca
    17641 ttggcggcat tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt
    17701 cgattccagc aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc
    17761 gcaaaatgac cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg
    17821 tagaaacctt tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt
    17881 cccacggccc gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc
    17941 tggaagaggt cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata
    18001 tgcagcaaac gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg
    18061 ggcagtaatg actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct
    18121 ggttttttga tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg
    18181 ggacaccccg caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca
    18241 ggttacgctt gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc
    18301 tcagcatatt ttgcagggtg aacaaggctt tcgcctgaca ccgctggcgt taaaggattt
    18361 tcaccgccag ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga
    18421 aaagcgcctg cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg
    18481 ctatctgatg gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg
    18541 cactatcgtt aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt
    18601 ttctatagat attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg
    18661 cgggcagcgc atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt
    18721 cgaactggaa tacgtcgcca gccgcccgcc gttgctggaa aaactcaacg cctggtttgc
    18781 caactacgat cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct
    18841 gcaaaaacat gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct
    18901 ggagtggcgc gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct
    18961 aattatcgac ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct
    19021 ggaaactgtc gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg
    19081 aatggacgaa attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct
    19141 gaaagattgc gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact
    19201 cgaacgagca acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt
    19261 tggtcatctc tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctaggcga
    19321 agtgccgccg cacgccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga
    19381 ttcagtgctg gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat
    19441 tgatcccgtc gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg
    19501 ttttctcgat gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat
    19561 ctggcacggg cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa
    19621 gatcattatg aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc
    19681 gcgcctggca tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt
    19741 gattgaagcg cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct
    19801 aaaaggtgca cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt
    19861 taacgcctgg tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga
    19921 gtatgaaact catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag
    19981 taaaaagcgc tacgccgggc tgattcagga gggcgacaag cagcggatgg tgtttaaagg
    20041 gctggaaacc gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctgta
    20101 cctgcgtatc ttccgcaacg agccatatca ggagtacgtc cgccaaacca tcgacaaact
    20161 gatggcgggt gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag
    20221 cgaatatcag cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa
    20281 tcaaaagcgc ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac
    20341 caccaacggc ccggagccgc tggactacca acgttcacca ctggattacg aacactatct
    20401 gacccgccag ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc
    20461 tacacttatg accgggcaac ttgggctatt ttgagcaaaa aaaagagttc gccagatacc
    20521 attttgatgc gtgacgaatg ctttgccatc cagtaccata gcgccctttc cattcctgga
    20581 cctgaataac gccactacct cataagcacg gtagcgggtg gttattgcct gcaattaaag
    20641 atatagagcc gaacacatat gccttttaca cttggtcaac gctggatcag cgatacagaa
    20701 agcgaattgg gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc
    20761 ccatctactg gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg
    20821 ttcaaccctg gtgataccat taccagccat gacggctggc agatgcaagt cgaagaagta
    20881 aaagaagaaa atggcttgct gacctatatc ggtactcgcc tggatactga agagtccggc
    20941 gtagccctgc gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt
    21001 ctgtttgccg ggcagattga ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag
    21061 tattccagcg aacagttccg tatgccgtac agcggcctgc gcggtcagcg taccagcctg
    21121 atcccgcatc agctcaacat cgctcatgat gttggtcgcc gccacgcgcc gcgcgttctg
    21181 ctggctgacg aagtgggttt agggaaaacc attgaagccg ggatgatcct gcatcagcaa
    21241 ctgctctctg gcgctgctga acgtgtgctg attatcgtcc cggaaacctt acagcatcag
    21301 tggctggtag aaatgctgcg ccgtttcaac ctgcgttttg cgctgtttga tgatgagcgt
    21361 tatgccgaag ctcagcacga tgcttacaac ccgttcgaca ccgaacagct ggtgatttgc
    21421 tcgctggatt ttgcccgtcg tagcaaacag cgcctggaac atctctgtga agccgaatgg
    21481 gacctgctgg tggtcgatga agcgcatcac ctggtgtgga gcgaagacgc gccgagccgc
    21541 gaatatcagg ctattgaaca actggcagag cacgtgccgg gcgttctgct gctgaccgca
    21601 accccggaac agttgggaat ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac
    21661 cgtttccacg attttgcgca gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac
    21721 gccgttgcca tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa catgcttggc
    21781 gagatgatcg gcgagcagga tatcgagccg ctgttgcaag cagcaaacag cgacagcgaa
    21841 gatgcccaga gcgcccgtca ggaactggtt tcgatgctga tggatcgcca cggcaccagc
    21901 cgcgtgctgt tccgtaacac ccgtaacggt gtgaaaggat tcccgaaacg cgagctgcac
    21961 accattaagc tgccgctacc gacgcagtat cagacggcta ttaaagtctc cggcattatg
    22021 ggcgcacgta aaagtgcgga agatcgtgct cgcgatatgc tctacccgga gcgtatttat
    22081 caggaatttg aaggtgataa cgccacctgg tggaacttcg atccgcgcgt tgagtggctg
    22141 atgggctacc tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc
    22201 actgcgctgc aactggagca ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc
    22261 cacgaaggta tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac
    22321 accggcgcac aggtactgct gtgctcagaa atcggttctg aaggacgtaa cttccagttc
    22381 gccagccaca tggtaatgtt tgacctgcca ttcaacccgg atctgctgga gcagcgtatt
    22441 ggtcgtctgg atcgtatcgg tcaggcgcac gatattcaga tccatgtgcc ttatctggaa
    22501 aaaactgctc agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc attcgagcac
    22561 acctgcccga ccggacgcac tatttacgat agcgtctaca acgatctgat taactatctg
    22621 gcttcaccgg atcaaaccga aggctttgac gatctgatca aaaactgccg cgagcaacat
    22681 gaagcgctga aagcacaact ggaacagggg cgtgatcgcc tgctggaaat ccactccaac
    22741 ggtggcgaaa aagcccaggc actggcagaa agcattgaag agcaggatga cgataccaac
    22801 ctgatcgcct tcgccatgaa cctgttcgat attatcggta tcaatcagga cgatcgcggc
    22861 gacaacatga tcgtgctgac cccgtccgat catatgctgg tgccggactt ccctggcctg
    22921 tcggaagatg gcatcaccat cacctttgat cgtgaagtgg cgctggtgcg tgaagatgct
    22981 cagtttatta cctgggaaca tccgctgatc cgcaacggtc tggatctgat tctttctggc
    23041 gataccggta gcagcacgat ttcactgtta aaaaacaaag cgttgccggt aggtacgctg
    23101 ttggtggaac tgatttacgt ggtcgaagcc caggcgccga agcagttgca gctcaaccgc
    23161 ttcctgccac cgacgccggt acgtatgctg ctggataaaa acggcaacaa cctggcggcc
    23221 caggtggagt ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca caccggcagc
    23281 aaactggtta acgccgtgca gcaggatgtt cacgctatcc ttcagctggg tgaagcgcag
    23341 atcgagaaat ctgcccgtgc attgattgat gccgcacgta acgaagccga cgaaaaactg
    23401 tctgccgagc tgtctcgtct ggaagcgctg cgcgcagtga acccgaacat tcgtgacgac
    23461 gaactgaccg ccattgagag caaccgtcag caggtaatgg aaagcctgga tcaggcaggc
    23521 tggcgtctgg atgccctgcg tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg
    23581 ggatggaaaa ctacaatcca ccgcaggaac cctggttggt tatcctgtat caggatgacc
    23641 acattatggt ggttaacaag ccgagcggtt tgttgtctgt gccgggtcgt ctggaagagc
    23701 acaaagatag cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc
    23761 gtctggatat ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg
    23821 agttaaaacg ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg
    23881 ggcatccatc ccccgcagaa ggtctggtgg atctgccgct gatttgcgac tggccaaacc
    23941 gcccgaaaca gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg
    24001 tggagtatgt ggcggataac acggcaagag tggtgttaaa accgattacc gggcgttcgc
    24061 atcaattgcg tgtgcatatg ctggcgctgg gtcatccgat tctcggcgat cgtttttatg
    24121 catcaccaga agcgagagcg atggcaccac gtttgttgct acatgcagag atgttgacga
    24181 ttacccatcc ggcgtatggc aatagtatga cgtttaaagc gccagcggat ttttaatcgt
    24241 cacgctggcg cagttcggta ggcggataag gcgttcacgc cgcatccggc aattgtgcgc
    24301 cgattgcctg atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa acttatttaa
    24361 accctttctg ctgctttatc agctcatatg cctgctgaat ttcctgcgct ttctgcttcg
    24421 ccatctccat catctcaggc ggcaaacctt tcgccaccag cttatcggga tggtgttcac
    24481 tcatcagctt acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca
    24541 gcacattaca ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc
    24601 cagattgctg ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact
    24661 ggtcaaactg ggcgcgggag atccctaatt cttcagcaat gacatacagc accgcccgtt
    24721 cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac
    24781 gaattaagtc aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg
    24841 ggtaattatc tgattttccc actcggaacg cgttttgcgc cgcagtacgg gaagcgccat
    24901 gaaggttcat tcggtccatc aactggctgg cgatatgaat atcagcctcc gtgacgcgac
    24961 ctttggattt ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac
    25021 gctcacgctg gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc
    25081 caattaacag gcctaacact acgccccaaa agccgccgcc catcagtaag gccacggcca
    25141 cgccaatgat ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg
    25201 ctatctccca caatataaag gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca
    25261 tcgcctataa tttgcattat catacctgtc attcaatgcc gtgcctaaca ccactgacgc
    25321 tattcggaca ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg
    25381 tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc
    25441 actctcctgg ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac
    25501 ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat
    25561 accaatgact tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac
    25621 gccgtgttta ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa
    25681 gtgcagctcc atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg
    25741 ctcggtaatg tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg
    25801 aatctgaaca ccaaagatac caacgtctgg gaaggtgatt accagatggt gggtcgccag
    25861 ggtcgcggta aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat
    25921 aacggtagct ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa
    25981 attattcatg accgcgaaga acaagttgcg gagatctgga acgcccgctt taaggtgggt
    26041 ccggtaccga tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct
    26101 ggtttcttga tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca
    26161 tattaccgga acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt
    26221 cgtggcaaca tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg
    26281 atggaactgg actatctgcc ttcagataaa gtctatgaag atgaacaccc gaacgatgac
    26341 agttcacgtc gttggttatt ctactggaac cactccgggg tcatggatca ggtgtggcgt
    26401 ttcaacgtcg actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag
    26461 tacggttcca gtactgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa
    26521 aacttcaatg ccaccgtttc aaccaagcag ttccaggttt tcagcgaaca gaacaccagt
    26581 agctactcgg cagagccgca gttagacgtt aattactacc agaatgatgt tggtccgttt
    26641 gatacgcgta tttacggcca ggcagtgcac tttgttaaca ccagagacga catgcctgaa
    26701 gcaacccgtg ttcacctgga accgaccatc aatttgccgc tctctaataa ctggggcagc
    26761 atcaataccg aagcgaagtt gctggcaacc cattatcagc aaaccaatct tgactggtat
    26821 aactccagaa acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa
    26881 gttgacggca aaatggtctt tgaacgcgat atggaaatgc tggctccggg ttatacccaa
    26941 acgctggaac cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag cgacatctat
    27001 aactacgact cgtctctgct gcaatctgac tactctggcc tgttccggga ccggacttac
    27061 ggcggtcttg accgtattgc ctccgctaac caggtgacga ccggtgtcac atctcgcata
    27121 tatgatgatg ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg
    27181 gagtctcgca ctggcgatga caacataaca tgggagaatg acgacaaaac gggttcactg
    27241 gtgtgggcag gcgatactta ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt
    27301 cagtacgata cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg
    27361 gatgaagacc gtctggtaca gctgaattac cgttacgcca gcccggaata tattcaggct
    27421 acgctgccta agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt
    27481 gctgtcgcca gctggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac
    27541 accaatgcga acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat
    27601 gcaattcgcg tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg
    27661 gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt
    27721 ctgggtacgc aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt
    27781 gttgatttac cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg
    27841 gaaaacgctg cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt
    27901 agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg
    27961 attaatgcag tcggtaaaac tgaacgctgc tcaggcaagg cagcaacttc ctgatgacgc
    28021 gacgctgcgc caccaaatca tggaacgttt gatcatggat caaatcatcc tgcagatggg
    28081 gcagaaaatg ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc
    28141 gaaacagaac aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggactgaa
    28201 ctacaacacc tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa
    28261 cgaggtgcgt cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt
    28321 gggtaaccaa aacgacgcca gcactgagct gaacctgagc cacatcctga tcccgctgcc
    28381 ggaaaacccg acctctgatc aggtgaacga agcggaaagc caggcgcgcg ccattgtcga
    28441 tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca
    28501 ggcgctgaac ggcggccaga tgggctgggg ccgtattcag gagttgcccg ggatcttcgc
    28561 ccaggcatta agcaccgcga agaaaggcga cattgttggc ccgattcgtt ccggcgttgg
    28621 cttccatatt ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgaccga
    28681 agttcatgct cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg
    28741 tgtgaaactg gaacagattg ctgctgatat caagagtggt aaaacgactt ttgctgccgc
    28801 agcgaaagag ttctctcagg atccaggctc tgctaaccag ggcggtgatc tcggctgggc
    28861 tacaccagat attttcgatc cggccttccg tgacgccctg actcgcctga acaaaggtca
    28921 aatgagtgca ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg
    28981 taatgtcgat aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg
    29041 taagttctcg gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa
    29101 aatcctgagc aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc
    29161 gggattggcc cggacttagt tgtccagctt gcacagcgtg agtggccggt cgaactggtt
    29221 gtttgtgccg atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc
    29281 ctccgctctt attcccccaa ctcccctgca caaccgcaaa ctacgggcac attaacgcta
    29341 cttcctgtcg cgctacgtga atctgtcact gcggggcagt tagcgattga aaatggacat
    29401 tacgtggtgg agacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg
    29461 ctgatcacag gccccgtgca taaaggcgtc attaacgacg caggcattcc gtttaccggt
    29521 cataccgagt ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc
    29581 gaagaacttc gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct
    29641 atcacccctg cacttttgca cgaagtgatt gctattttgc atcacgattt gcgtacaaaa
    29701 tttggtattg ccgaaccgcg cattctggtc tgcgggctga atccgcacgc gggcgaaggc
    29761 ggtcatatgg gtacggaaga gatagacacc attattccgg tgctcgacga gctgcgggcg
    29821 caggggatga aactcaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc
    29881 gataacgccg acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac
    29941 cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg
    30001 gaccacggca ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg cagttttatt
    30061 acggcgctta atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc
    30121 agggccactt agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg
    30181 acagcattgt ttctgccatt aacccgcaaa agggccaggc aatggtcgaa atcggccccg
    30241 gtctggcggc attgaccgag ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac
    30301 ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga
    30361 tttatcagca ggatgcgatg acctttaact ttggcgaact ggccgagaaa atgggtcagc
    30421 cgctgcgtgt tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt
    30481 ttagctatac tgatgccatt gccgacatgc actttatgtt gcaaaaagag gtggtgaatc
    30541 gtctggttgc aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact
    30601 attgcaacgt gatcccggta ctggaagttc cgccgtcagc ctttacacca ccacccaaag
    30661 tggattccgc cgtcgtgcgc ctggttcctc atgcaacgat gcctcacccg gttaaagatg
    30721 ttcgtgtgtt gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta
    30781 acagcctcgg caacctgttt agcgtcgagg tgttaacggg aatggggatc gacccggcga
    30841 tgcgagcgga aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga
    30901 acgcgccttt gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca
    30961 aagcgtctac attgaggcac aatcttcacc tgataatgaa cgttacgttt ttgcttatac
    31021 cgtaaccata cgcaatctgg ggcgaacgcc agtgcagttg ttggggcgtt actggctgat
    31081 caccaatggc aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc
    31141 acttatcgcg cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct
    31201 gggcaccatg cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga
    31261 cattcccgta ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata
    31321 ccttattggc gacgttcatg gttgttacga tgaactgttc gcattgctgc ataaagtaga
    31381 atttacccct gggaaagata ccctctggct gacgggcgat ctggtcgcgc gcgggccggg
    31441 ttcgctggat gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg
    31501 caatcacgat ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga
    31561 tcgcctgaca ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg
    31621 ccagcctttg ctgcaaatcg acgaagagaa aaagttggtg atggcccacg cagggatcac
    31681 gccgcagtgg gatctacaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctatc
    31741 gagtgactcc tatcccttct ttctcgacgc tatgtacggc gatatgccaa ataactggtc
    31801 accggaattg cgggggctgg gaagactgcg ttttatcacc aacgccttta cccgtatgcg
    31861 tttttgcttt ccaaacggtc aactggatat gtacagcaaa gaatcgccgg aagaagcccc
    31921 tgccccactg aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat acagcatcgc
    31981 ctttggtcac tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt acgcgctgga
    32041 taccggctgc tgctggggtg ggtcattaac ctgcctgcgc tgggaagata agcagtattt
    32101 tgtccagccg tcgaaccggc ataaggattt gggtgaggga gaggcggtcg cgtcttaacg
    32161 aattaccatt cagggaggaa taatggctga ctattttgaa atacgtgctg attacaatca
    32221 gcacaccatc acgatttatc aggcttacaa tgatgcaatc gctgacgtcg ccgtacgtga
    32281 cgggcgattt ggcgcacctt tttcattcaa ccgcatgacc tggattaaac cctcttttat
    32341 gtggatgatg gagcgtagca actggggact gaaaaaagat caacaacaca ttctcgcaat
    32401 ccgaatcaaa cgcaccttct ttgatacatt gctggaacaa gcggtgctaa ccacgccgga
    32461 agcccatgtg tatccacatg caggaatttg ggaaacgtta tttgcacaag caaatgtgta
    32521 tgttcagtgg gacccagaac gcagcataaa tggtaaaaaa ctggagcacc gatcattaca
    32581 gcttggtatt tctcgtaact tgatcagtca atttaacgaa gacgcgattg ttgctattga
    32641 tgatctgacg ccgctggtgc gaaagtgtca taaccttttg ataaacggca aaacaacaca
    32701 ggcgaaatcc ttcctgccgc cagaaaaaat ttacccagtt agtgcagcag cgagaaaggc
    32761 tttgggaatg aaaaattcct gagacaaata cgcacctatt aagcggaaaa tatctaagtg
    32821 cgacaaatca acaccgggct taataccaga gaaattattc tggaaatgat tgacgaagca
    32881 gatgcccccc cgtttgctga gcagcggacc tggcatcgca ggcgaatcgc ttttctatgt
    32941 gattgcatca gcgttgctgt cgactacggc ttcttctgtt ctgtctctca gcagtcaata
    33001 atgaacataa ccaagatggc tgaagatgta tggataacag aaagtaaaac gacccaactc
    33061 attgatattt ggcaacaact taaacagctt tatccgccag aaactattcc ctacgcccag
    33121 aagttaccca ctaaggaatt gcttgaactg ctactttcta gtcctgacga tcttcgaata
    33181 attgaaattc ctgacatcat cggtgatgtt tttgatgacc aaacctggat tcgattattc
    33241 aaaaaacact ttgctgatgt tattcagcta cgatgtgatg tgtaacaatc caaaatatat
    33301 cgccagacga tagaccaaaa agaaagtatt tagcagatac tcaacattct gacttctgta
    33361 cagaaatagc agagcttaaa aacttaaaac ttccggtact catcagagaa acatccagtt
    33421 tcttctgcaa attcatttaa tgccgagatg gcatcctcgt ttacttcatt ccactgtaga
    33481 gtggcttgaa ggtttagacc tacgcaatga gcgcgactca caaaatcgcg ctcactggta
    33541 aaatcaaacg tcttatcgtt caatttgtag tcctggcaaa aaattaccgc cgctccagaa
    33601 tctcaaagca atagctgtga gagttctgcg catcagcatc gtggaattcg ctgaataccg
    33661 attcccagtc atccggctcg taatccggga aatgggtgtc gccttccact tctgcgtcga
    33721 tatgcgtcag atacagtttc tgcgcttttg gcaggaactg ctcataaacg cgaccgccgc
    33781 caatcaccat gatttctggt acgtcaccac acgccgcgat ggcttcatcc accgacttca
    33841 cccacgttac gcgatcgtcc gtacccggtt gactgctgag gataatattt ttgcgtcctg
    33901 gcaacggacg accgattgat tcccaggtat ggcggcccat aatcacgggt ttatttaagg
    33961 tgttgcgttt aaaccaggcg agatcggcag gcaggttcca cggcatggcg ttttccatgc
    34021 cgataacgcg atctaccgct aacgccgcaa tcagactgat cattgagatt tcccgataaa
    34081 aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt
    34141 tgaggaaaat tttatattct gctggcgagt ccacgctctc tccctggact cgccgcatta
    34201 caatgaaaca aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattagga
    34261 tgagggtttc gtttccggtt catccgccat gttgccggta tgtttacctt cttcggttcc
    34321 ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtaa tgatctcact
    34381 taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcgt tctcaaccat
    34441 tgccatctct tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc
    34501 ttcatacggc cccagaccta aactttccag tgccagacgc ccggttttca gcgcaccttc
    34561 gaaggtttca cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtggtcgac
    34621 atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag
    34681 ttgcaggttg gtttgcggat catcaatagc attaatcagc acttccgctt tcgccgctcc
    34741 ggcagattcc agtaaatcca tccgcgttgc atcgccataa aacactttca taccaaattt
    34801 acgcagcgtt tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga
    34861 gagcagtaaa cgtccggtaa tctgcccaaa acgaccgaat ccggcgataa tcacgcgcgg
    34921 ctgttcttcg tcgatctcat cggcttcacg cgcttcctca gtagaagatt gctcaaggcg
    34981 attgaggatc accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag
    35041 cgatttcgcc cactccggct ccagcacatt cgccatctgc gccgcgccaa ataccacaaa
    35101 ggcaaactca ctgccctgcc ctaacaacac cgcaaaccaa cgacgctgtt tatttggcac
    35161 ttgcaacggt cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag
    35221 caaaatgaca atgcgcaatg ggttttcaag cagcgtgcca aagtctatcg acatgccaac
    35281 accgatgaaa aacagcccca acagcaaacc tttaaatggt tcgatatcgc tctccagcgc
    35341 atgacggtat tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga
    35401 caagccgacc tcttccagca gcaaaccaaa gccaaacacg aggaataacg ccacggcact
    35461 aaacacttcc cgcaagccag agcgggctac aaaacgcagc gccggacgcg tgacatagcg
    35521 ccccagcaat accaccagca ccagcgcacc cgccactttt aacgccgaga gagcaaatgc
    35581 gcccatcgtc gtcgaggcac tgctcgttgc cagtagcgga atcatcgcca ccagcgggat
    35641 cgccgcgata tcctggaaca gcagcaccgc aaaggcactg cgacccattt gcgtcaccat
    35701 cagattgcgt tcattcatcg cctgcatggc aatcgccgta gaggagagcg ccagcgtcat
    35761 gccgatcaat tccgcgacct gccagcgcag cccaagtaac atgcagaaca gccccagcag
    35821 gccgccgcaa atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca
    35881 cagcctttgt ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc
    35941 ggcaaagtgc agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat
    36001 gcagccggcg atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac
    36061 aatcagcgct gccgaaccga gataaatcag cgcctgaatc agcgtatggc tatccatgat
    36121 gggcctcctg ccattccagc agacgttgct tatagtgacg cgcctgccct tcgagggttt
    36181 cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg cagccagttc agtccgcagt
    36241 agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt
    36301 caaaatggct ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg
    36361 ccgtgccgcc atgaccgtaa gcccagccgt gggagaaaac tttatcgatc caaagtttga
    36421 ggagcggagg aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag
    36481 acagcgcctc ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag
    36541 agcgaatttc gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg
    36601 aatgatgcgg atacggatgc gcataaatta taagaatcat aggtagcccg cgtgattacg
    36661 cctttgtttt cccacaaagt gtagtcatat attcacgcgg cttatagtca ataatattga
    36721 ttcagaaaag agagaaatct gaacaattta ccccattctc tgcccgcgga tcgtcatatt
    36781 gctggtttgc gccagtgaca tccctcgagt ctccggggca aacgctacgg aaatcaacaa
    36841 gccaaacagc gagatacccg cccccattag catcgtgtta ctgataccgt aattattgat
    36901 aaagatcggc agtgcccagg tcgaaacaat ggtgccaata cggcttaagg acataatcac
    36961 gcccacggca gaggcgcgga tatctgtcgg gaagagttca ttaggataga gccactgcaa
    37021 attacccggc ccgccagaga aaaaggcata caccgcaaag gccatcacta ccagccagat
    37081 ccccatatcc gggatcagcc ccaaaaccgc cagcgccagc gtcatcatgg caaagctgcc
    37141 aatcaacaat ggacgccgtc cggcagtgtt taaccacagc atcggcggaa tacagccgag
    37201 cataaagaac aggctaatca ccacgttccc cagcgccgcg tttttgccaa cacccaatcc
    37261 caacaaacca acgatttgcg ggccaaaggt gtaaatggcg aacattggga tcacctggca
    37321 ggtccagatg gcggcaacaa acagaacaaa aggaaaatgg cggcgattaa acagatcgcg
    37381 aaaacgggtt tgctgcggct gctcttcatc gaaagccacc ggttcgccaa acagttttat
    37441 catcatctct tcgcactctt ttactcgccc tttgcgtaat aaccagcggg gagattcagg
    37501 cagttcgaat cgaccaatca aaatcaacaa acaggggatc gccgcgctac ccagcatcca
    37561 gcgccagccg ccttccacat cataaagcca gtagccgacc agatcggcgc aggtcgcgcc
    37621 gacataccac atggcggcga taaagctgat ggaaaacgcc cgctgacggg tactggagaa
    37681 ctcggtgatc attgaggtgg cgatgggata atctgcaccg atgacaatgc cgataagtac
    37741 ccgcatcacc aacagttcga cgggggatga aacaaacatc gtcgccaccg atatcacgcc
    37801 gatggcgatg atatcaatga ggaacatttt gcgccgtccg actttatcgg aaatatagcc
    37861 aaacagcgat gtgccaacga acagcccggc gagcgttccc gcgcccaaca agccaatcca
    37921 gtcagcgtcc agttttagcg ccggagtcag ttgctccagc gccacgccaa tcattaccag
    37981 tacataacca tccagaaacg gaccaccgct tccccacagc aaaatgcggc ggtgaattga
    38041 ggagaatttg agatcgtcaa agtttctgga cggttgcatg ggttattcct gtttttttgc
    38101 aatgagagtc agtacggcgt tacgtatcaa accaacatca gccgtaacgg aactccacac
    38161 caaaggtgcc gcgcgggtat tcccactgtt ccagcgccga ccccagcccc agaatgcgac
    38221 aggtgccgca ctccagacat ccggcgtaat cgaagcgcac actgccgtca tcctgcttct
    38281 tgtacagacc tgcggggcac gctttcacca gcagctccag ggcctgttta tcggcatcgg
    38341 ccttaacaac aatgtgcgga tgctcttcat cgacattgaa tttattgacg cccagtttga
    38401 cgtccacatt gacgggagaa gtcataaaac ggtcactcct ttcatgccat ccttgatcag
    38461 attgatgaag cccactttct tgccgtggcg gaggattttc ttgcgcatca gttccggcgc
    38521 actgccgtca atggtgaaca ggtcacgcgc cacgcccacc gccagttccg ggtagccgct
    38581 aaacatgcgt gggttatcaa ggaaggccgg tagtttctgg tacatacgca tatcgcgcag
    38641 cgggccactc tcaagatgct gacgatattc cgcgagtttt tgcttactga aatcgtcgct
    38701 tttcatcgct gaaagcacgg tttttgctgc agcttccccg gcggcaatcg ccagatccat
    38761 accgcgaatg gtaaaaccga ggttcataca cattccggcg gcatcaccgg caatcaatac
    38821 gccgtcacca accaactccg gcagcatgtt gatgcctgct tccggcacta cgtgagcgga
    38881 atattccacc agcttgccgc ccgcgatcag cggtgccact gccggatgct gtttgaaatc
    38941 ttccagcatt tgcggcaccg atttttttgc gtcatgcaga tgatgcagac cacaaaccag
    39001 ccccagcgac agggtatttt cattggtata aaggaagccg ccgcccatca ggccatcggt
    39061 gggtgatccc gcaaacagac aagccgcgcc ctgattaccc tgcaactgaa aacggtcttc
    39121 gattaccgac ttcggtaact cgatcagttc cttcacgcca accgccacat ccgtcggttt
    39181 aacgcgtttt gccatcccca gcttttcggc aaggatggag ttcactccat cagcaaggat
    39241 caccgttttc gcttcaatca catcgccatc ggcttctaca ccgacgactt tgccatcgcg
    39301 ctgtacgagg ttatcgacgc ggatcccggt aattaactgc gcgcccgctt cttcggcctg
    39361 ctccatcagc caggcatcaa atttactgcg caaaacggag taagaacgct gggatggcga
    39421 ggtttcgtca ccattgcagt agtccatagt catcgctgac ttttccgtca taaacgcgag
    39481 tttttcatgg gtgatcaggc gttctacggg ggcggagtcg gcgaaaccag gaataatgtg
    39541 ttccaggctg tgggcataga gacgcccgcc ggtgacgttc ttggcacctg cggaattgcc
    39601 acgctcgata actaacactt gcgccccttc gcgggcgagc accagtgcgg caaccgaacc
    39661 ggcaagccca gcaccgacga tgatggcgtc aaagatatct tcggacataa gagctacctg
    39721 tcagcggcag ggccaaaatg cagccctgcc agagtggatc aacgcgctaa agctgcggtc
    39781 agcgccggaa ggatcttcac ggcgtcacca acaatgccgt aatccgcgta ctggaagatc
    39841 ggcgcatttt tatctttatt gatggcgaaa atggtttgcg atgcattagc accaaccatg
    39901 tgctggatct gcccggagat ccccaccgcc aggtacagtt caggtttcag catcaggttg
    39961 gagataccga catagcgttc gtgctccatc catttttcgt tttccgccac cggacgagaa
    40021 caggccaact ccgcacctat cgccttgcaa agctgttctg ccagcgcaat gttctctttg
    40081 ctgccaatac cgcgaccgac gctgaccacc agacgggctt tgtcgagatc gacgctgttg
    40141 ctctggcgcg cctgggttgc cgtgcgggta atcgccacag ccggagcctg ccactccacg
    40201 gtgtgcgttt cgccagtgcg tgacgcgtct ggctgagccg catcgaacgt gccgctgctg
    40261 atggtcagta ccgcatacgg cgtggcaatg cgttcttcgc caatcgccag accaccgtaa
    40321 accatgtgtt tcactgtcgc tttaccgtcc tgtacgctga cggtgctggc atcgttagac
    40381 accgccgctt taaggcgata acccagtttt gccgccagta atttgccgcg acgggtgttt
    40441 ggcagcagca ccaggccgtc tgcgccgtgc tggcgaatag tgtcagccat gacaccggcg
    40501 taatcttcga tcatccgatc gtccggtttg ccgtttaatt tccagacatg attagcgccg
    40561 agctggattg cctgtacgcc gtcggcatca ttgaggacaa aggtgttgat ttgattagct
    40621 aaagcctgcg caccgttcat cagttccggc agacgagaag gggtatcgct gaatacccag
    40681 acttgagaaa acgtgttcat agcatcccct gtaattaaat gactttgcga agattttcag
    40741 caaatgcggc gatctgttct tcgccgtcgc cttcaatcac gatgcgctga cgttcgcgct
    40801 gtttcggcgc agcaacctgt tgttcagacc aggcgtccac ggcgttaaaa ccgatatccg
    40861 ccgccgacca tacctggact ggctttttcg ccgcgccgag aatggctttc atcgaaggaa
    40921 tttgtgggga gttgatatca gtggaaacag caacaaccgc aggcagcgga atgcttaagg
    40981 tttcggtttc atcttccagt tcgcgctcaa cggtgagggt atctgcagtc agggagataa
    41041 ttttgctgac gccgttaact gccggaatat tgaggatttc gcccaccagc agaccaacct
    41101 gctgggcata aaggtcggaa gaaccatcgc cacagaggat caaatcaaag cctgctttct
    41161 gggcggctgc agccagtgcg ctcgccgttt gttgcggcag tgcctgctcg aactggtcat
    41221 caatcaccac aatcagttca tccgggccgc gcgatagcac atctttacgc cctttggcgt
    41281 tggtcagggc tttaccgccc acacttaagg ccgtcacctg cgcctctgct gcctgttgct
    41341 ttagctggca agccgcttca atagcgttca gatcgtattg gcttattttg gcatcggctt
    41401 tgctgaagtc taatgaacca tcagcattat tgaccgcaat atcctgttca tcaggcacgc
    41461 acttatagca agtaataatc ttcattgcat ctccagaaat catgaaggaa acgtcgttca
    41521 tatcggtggt gttaattaca gatatcacac cctttaattt ggaaataaaa ctttaatcac
    41581 caatattgaa aatgtcacac gcagataaat ccaactttca atattgttaa gttcctcacc
    41641 aatattgaaa acatcgcgta gcaaaaagaa attttcaata ttgctttatg gatcaccaat
    41701 attgaaagtc agatgctatt tttttactca caacacaaca taacaaactg attaataatc
    41761 aaaattaacg agaaaagcgc cctctattca cccgaaacaa aaatgtgata acaatcacag
    41821 aatgcagct
//
LOCUS       DAZGTR010000026        41098 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGTR010000026 DAZGTR010000000
VERSION     DAZGTR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.026"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            281..586
                     /locus_tag="ACHLCY_001593"
     CDS             281..586
                     /locus_tag="ACHLCY_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0368884.1"
                     /translation="MATANKNAKSQLTTVRVPLDVMQGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENPLVSSLDALAKVEQIGIKAAEEIGQLVTVAREELQRRKTKEPE"
     gene            complement(481..1029)
                     /locus_tag="ACHLCY_001594"
                     /pseudo
     CDS             complement(481..1029)
                     /locus_tag="ACHLCY_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1082..1270)
                     /locus_tag="ACHLCY_001595"
     CDS             complement(1082..1270)
                     /locus_tag="ACHLCY_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0368885.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(1645..2874)
                     /locus_tag="ACHLCY_001596"
     CDS             complement(1645..2874)
                     /locus_tag="ACHLCY_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0368886.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGAVKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWMERLGI
                     LAGTHENVTTLPVARRK"
     gene            2862..3044
                     /locus_tag="ACHLCY_001597"
     CDS             2862..3044
                     /locus_tag="ACHLCY_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368887.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            3051..3956
                     /gene="tqsA"
                     /locus_tag="ACHLCY_001598"
                     /pseudo
     CDS             3051..3956
                     /gene="tqsA"
                     /locus_tag="ACHLCY_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(3981..5369)
                     /gene="pntB"
                     /locus_tag="ACHLCY_001599"
     CDS             complement(3981..5369)
                     /gene="pntB"
                     /locus_tag="ACHLCY_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP0368888.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5380..6912)
                     /gene="pntA"
                     /locus_tag="ACHLCY_001600"
     CDS             complement(5380..6912)
                     /gene="pntA"
                     /locus_tag="ACHLCY_001600"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP0368889.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7436..8380
                     /gene="ydgH"
                     /locus_tag="ACHLCY_001601"
     CDS             7436..8380
                     /gene="ydgH"
                     /locus_tag="ACHLCY_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP0368890.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8566..9948
                     /gene="ydgI"
                     /locus_tag="ACHLCY_001602"
     CDS             8566..9948
                     /gene="ydgI"
                     /locus_tag="ACHLCY_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0368891.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9985..10707
                     /gene="folM"
                     /locus_tag="ACHLCY_001603"
     CDS             9985..10707
                     /gene="folM"
                     /locus_tag="ACHLCY_001603"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP0368892.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10704..11039)
                     /gene="ydgC"
                     /locus_tag="ACHLCY_001604"
     CDS             complement(10704..11039)
                     /gene="ydgC"
                     /locus_tag="ACHLCY_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP0368893.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11168..11887
                     /gene="rstA"
                     /locus_tag="ACHLCY_001605"
     CDS             11168..11887
                     /gene="rstA"
                     /locus_tag="ACHLCY_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP0368894.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11891..13192
                     /gene="rstB"
                     /locus_tag="ACHLCY_001606"
     CDS             11891..13192
                     /gene="rstB"
                     /locus_tag="ACHLCY_001606"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP0368895.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13268..14197
                     /gene="tus"
                     /locus_tag="ACHLCY_001607"
     CDS             13268..14197
                     /gene="tus"
                     /locus_tag="ACHLCY_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP0368896.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(14194..15597)
                     /gene="fumC"
                     /locus_tag="ACHLCY_001608"
     CDS             complement(14194..15597)
                     /gene="fumC"
                     /locus_tag="ACHLCY_001608"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP0368897.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAVSLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15740..17386)
                     /gene="fumB"
                     /locus_tag="ACHLCY_001609"
     CDS             complement(15740..17386)
                     /gene="fumB"
                     /locus_tag="ACHLCY_001609"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0368898.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17585..18760
                     /gene="manA"
                     /locus_tag="ACHLCY_001610"
     CDS             17585..18760
                     /gene="manA"
                     /locus_tag="ACHLCY_001610"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP0368899.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18861..20369
                     /gene="ydgA"
                     /locus_tag="ACHLCY_001611"
     CDS             18861..20369
                     /gene="ydgA"
                     /locus_tag="ACHLCY_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0368900.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(20414..21679)
                     /gene="uidC"
                     /locus_tag="ACHLCY_001612"
     CDS             complement(20414..21679)
                     /gene="uidC"
                     /locus_tag="ACHLCY_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP0368901.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTWWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(21718..23091)
                     /gene="uidB"
                     /locus_tag="ACHLCY_001613"
     CDS             complement(21718..23091)
                     /gene="uidB"
                     /locus_tag="ACHLCY_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP0368902.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFLFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(23088..24899)
                     /gene="uidA"
                     /locus_tag="ACHLCY_001614"
     CDS             complement(23088..24899)
                     /gene="uidA"
                     /locus_tag="ACHLCY_001614"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP0368903.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     AEQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25288..25878)
                     /gene="uidR"
                     /locus_tag="ACHLCY_001615"
     CDS             complement(25288..25878)
                     /gene="uidR"
                     /locus_tag="ACHLCY_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP0368904.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAERMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(26099..26866)
                     /gene="hdhA"
                     /locus_tag="ACHLCY_001616"
     CDS             complement(26099..26866)
                     /gene="hdhA"
                     /locus_tag="ACHLCY_001616"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP0368905.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(26978..28006)
                     /gene="malI"
                     /locus_tag="ACHLCY_001617"
     CDS             complement(26978..28006)
                     /gene="malI"
                     /locus_tag="ACHLCY_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP0368906.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            28181..29773
                     /gene="malX"
                     /locus_tag="ACHLCY_001618"
     CDS             28181..29773
                     /gene="malX"
                     /locus_tag="ACHLCY_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP0368907.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVLALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            29783..30955
                     /gene="malY"
                     /locus_tag="ACHLCY_001619"
     CDS             29783..30955
                     /gene="malY"
                     /locus_tag="ACHLCY_001619"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP0368908.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            31059..32060
                     /gene="add"
                     /locus_tag="ACHLCY_001620"
     CDS             31059..32060
                     /gene="add"
                     /locus_tag="ACHLCY_001620"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP0368909.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKHALREKVAAK"
     gene            complement(32095..33135)
                     /gene="ydgJ"
                     /locus_tag="ACHLCY_001621"
     CDS             complement(32095..33135)
                     /gene="ydgJ"
                     /locus_tag="ACHLCY_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0368910.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            33378..33503
                     /gene="blr"
                     /locus_tag="ACHLCY_001622"
     CDS             33378..33503
                     /gene="blr"
                     /locus_tag="ACHLCY_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP0368911.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            33776..33991
                     /gene="ydgT"
                     /locus_tag="ACHLCY_001623"
     CDS             33776..33991
                     /gene="ydgT"
                     /locus_tag="ACHLCY_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP0368912.1"
                     /translation="MTVQDYLLKFRKISSLKSLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            34077..34517
                     /gene="ydgK"
                     /locus_tag="ACHLCY_001624"
     CDS             34077..34517
                     /gene="ydgK"
                     /locus_tag="ACHLCY_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP0368913.1"
                     /translation="MITTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            34594..35175
                     /gene="rsxA"
                     /locus_tag="ACHLCY_001625"
     CDS             34594..35175
                     /gene="rsxA"
                     /locus_tag="ACHLCY_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP0368914.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGHAIALITAGLMSLAFMGFSGLVKL"
     gene            35175..35753
                     /gene="rsxB"
                     /locus_tag="ACHLCY_001626"
     CDS             35175..35753
                     /gene="rsxB"
                     /locus_tag="ACHLCY_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP0368915.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            35746..37968
                     /gene="rsxC"
                     /locus_tag="ACHLCY_001627"
     CDS             35746..37968
                     /gene="rsxC"
                     /locus_tag="ACHLCY_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP0368916.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAIEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQSNAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            37969..39027
                     /gene="rsxD"
                     /locus_tag="ACHLCY_001628"
     CDS             37969..39027
                     /gene="rsxD"
                     /locus_tag="ACHLCY_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP0368917.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            39031..39651
                     /gene="rsxG"
                     /locus_tag="ACHLCY_001629"
     CDS             39031..39651
                     /gene="rsxG"
                     /locus_tag="ACHLCY_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP0368918.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            39655..40350
                     /gene="rsxE"
                     /locus_tag="ACHLCY_001630"
     CDS             39655..40350
                     /gene="rsxE"
                     /locus_tag="ACHLCY_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP0368919.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            40350..40985
                     /gene="nth"
                     /locus_tag="ACHLCY_001631"
     CDS             40350..40985
                     /gene="nth"
                     /locus_tag="ACHLCY_001631"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP0368920.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     LVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT         9997 a        10635 c        10079 g        10387 t
ORIGIN      
        1 gtgctcaggg cgataaaacg ccagatgtat ttattcaggt tgtgcgagtc ccgcccttgc
       61 gggtgtatgg tatgatttct catagctacc tcgatactct cgttatcgtt ggtggttaga
      121 cgccccgtat gtgtttccgg cactgcgggg cgttgctctt tgtatttcaa aaatcatttc
      181 ggtgtgtttc atgttatgag cgcatgaaac acacgtcaag gctttttgta tttctttttt
      241 tgtgtatact gaaacacacc gatgattagg agtttcagaa atggcaacag ctaacaaaaa
      301 cgcaaaatca caactgacaa ctgtcagagt cccactagat gttatgcaag ggatggaatc
      361 cgttaagctg gacggcgaaa gcaatgccgg atttatcgta accgccatgc gcggagaaat
      421 agcccgccgc caagcagaag gcagcggaga aaatcccctt gtgtcgtcac tggatgccct
      481 ggctaaggtc gaacaaatcg gcatcaaagc agcggaagaa atcgggcagc ttgtcaccgt
      541 tgcacgcgaa gaactccagc gtcgcaagac caaagaacca gagtaatcac catcagcgcc
      601 gtggtgtaag gtattacggc gcattgctat gcaggacaac acaatgaccg ataaagaatt
      661 gaccaaaaca ttatcaccgg cacggaaaag acggcgcaga aagatagagc atgaatcaga
      721 aagattcgcg ccatgtgctt ttgcccttga gcaattcctt aaagagtaca gggaaaagcg
      781 ctcattgcag gtatggcaac gaactgaacc agactgatag cattgcccac cagcctgata
      841 gcgactatca tttccgttct tatgtttggg atcacacaca caaacggcgc agcgggttat
      901 ctgttcagaa aggcggctcc atttcgggac cgcttttttt attcctgaaa aaccccaatt
      961 ttgttgtttt tcagtttcac cagggcgaac gaatccccgc ccacgttcgg gcgcatattc
     1021 aatcttcatg gttatatctc tgtattagtg gatgtgtggc ggctgcgtgc cgccagcgtg
     1081 attaatgaac tgccttgcag ctatccttcc aggccagaac ctcggataaa gaccagccaa
     1141 cggaacgacc gccaagttta cgacgtgatg ggaattgtcc ggccttttcc aggcggtagc
     1201 ggcatgagcg gctaaggcct gttagctttt cgcattcttt ttcacgtata aaccgatcag
     1261 tgcttaacac tattgccccc tttcgtttct taaagagtta tttcgtgttc tattgcgttg
     1321 ggatgtgtct gattgtgtca ggatgattca gagttggcaa atgttgaggc tgtatggttt
     1381 acagaaagag gaataatcag gataaaatca tttaaattca tgttaataca aaggcataaa
     1441 atattgttta atgccttttt tctcgctatt taaagagtga ttcgctagtg tataaaaaac
     1501 cagtaacaca ttaaaaatca gctacttata aatctgtaca ctttttcgcc tcttgttcgt
     1561 agttgttccg cattgttgct cattgttgca cgctgtatct gttcgcatat ccagtatgcg
     1621 catactgaaa aaatacgaaa aaaattattt tcttctggct actggtagcg tggttacgtt
     1681 ttcatgtgtt cccgccagta tccctaaccg ctccatccac atatccagcg cattgcgttt
     1741 agcatccaga taacgggaat gattataaac tcgctgcatt cctggcatct ggtggcctgt
     1801 aagctgctcc acgacatgcg gatcaacgcc taaatcgttc agcatggttg taaaggtgcg
     1861 ccggatgtca tgcagtgacc agtgagggtg attaagcctc ctgtgcgcta atcttccata
     1921 ctgcgatacg ctggcctcct gtttcactgc ccccagcaat aagcccgtgt gcctgttctg
     1981 ctccaccagc tgcgtgacga acggcaggat cgcttccggt atgggccgga atattgcgac
     2041 cttcgttttg ctgtgctcct tcggaacggt ccatagcatt tccgtaaaat cccactcccc
     2101 gatctccgat aacctcagtt ctaccgttct ggctccgaag acaatcagga ggcggattaa
     2161 cgcgacgtag taaggggaaa atattttttt atccagcgcc tgcaataact cgcccagttc
     2221 tttggtgctt aagacacgtt cgcttatatc cggttttttc ccaacgtccg ccacgttcag
     2281 atcgtccaga acgttgctga ttgcatagcg ccgcctacgg cagaacttaa gcgcctgttt
     2341 gcacgcctgt agcacgaatc cggcagtaac aggcgttcgc tttgccacct ggtcaaaaca
     2401 ggccagccag tgccgtagct cgcatttatc cagcggcata gcaccaatct gctgtattac
     2461 gtgattatta agtcgccttt tcagggcgat ataatccacg cggttttcct ttacgtaata
     2521 ctcaagccag taggtgagcg catcgccaac cgttacgggc tttaacgctt cctgtacggt
     2581 gtaattcatc tcatgacgtg gatttttccc ctcagccagc catgtgcgac actgtgcggc
     2641 tttttccctg gctgctttca ggctcagatc aggataactt cccagcttaa tgcgttcggg
     2701 tcgtgtctcc cttcccgttc cggccctgta tgtgaaatac caggtcaatt tccctgatgt
     2761 taaatatttc acgctcaggt ttccgccatc actataaaac gtgtttttct ccgctggctt
     2821 accatgaagt ttccttagca aggtatcgct cagtttgttc attgctctgc tcacgtttta
     2881 ccccctcttt acgaaaaagt gactacacca gtgactacac cgatcgttgc acaaagggct
     2941 acaacgtgaa acaagctgga acaaggaaaa tcacaaatct gttgataatc aaaaacataa
     3001 tgcacagcct gaaacattat gaaacagcaa aaaacactaa atgataaaat gctgttattc
     3061 ttaacccgct ggtgcaacac atggtccgct ggcgagtgcc gcgtgtactg gcggtgtcga
     3121 ttttgatgac catcatcgtg atggcgatgg tgttgctatt agcttatctg ggttccgcgc
     3181 tcaacgagtt gacgcggacg ttaccgcaat atcgcaactc tattatgacg ccgctgcaag
     3241 ctcttgagcc gttgttgcaa cgcgtaggga ttgacgtctc agttgaccag ctggcgcatt
     3301 acattgatcc gaacgcggcg atgacgttgc tcaccaactt attgacgcag ttatctaatg
     3361 ccatgtcatc aatattttta ttgctgctga cggtgctgtt tatgctgctc gaagtgccac
     3421 aattgcccgg aaaatttcag caaatgatgg cgcgtccggt tgaagggatg gcggcgattc
     3481 aacgtgcaat tgacagtgtt tctcattatc tggtgctgaa aacagccatc agcatcatca
     3541 ccggcctggt cgcctgggcg atgctcgccg cactcgatgt tcgcttcgct tttgtctggg
     3601 gattgctggc ctttgcgctt aattacatcc ctaatattgg ttcagttctc gcggcaatcc
     3661 cccctatcgc tcaggtactg gtgtttaatg gcttctacga agcgttgctg gtgctggcgg
     3721 gatatctgct gattaatctg gtcttcggca atattctgga gccgcgcatc atgggacgtg
     3781 ggctggggct ttccacattg gtggtatttt tgtcgttgat tttttgggga tggttgttag
     3841 gaccggtggg tatgctgctt tccgtgccgt taacaattat tgtcaaaatt gcgcttgaac
     3901 aaacagcggg aggtcaaagc atcgccgtta tgttaagcga tctcaataaa gagtgacggc
     3961 ctccgcagag gccgtcaggg ttacagagct ttcaggattg catccacgct ggctttggcg
     4021 tcaccaaaca gcatgtgggt gttttccttg aagaacagcg ggttttgcac accagcatag
     4081 ccagtgttca tcgaacgttt aaagacaatc acgttctgcg ctttccacac ttccagcaca
     4141 ggcataccag caataggact cttcggatca tcctgcgccg ccgggttaac cgtatcgtta
     4201 gcaccaatca ccagtacggt atcggtatca gcaaagtcat cgttgatctc gtccatttcc
     4261 agcacgatgt catacggtac ttttgcttca gccagcaata cgttcatatg tccaggcaaa
     4321 cgccccgcaa ccgggtggat accgaaacgc acgttgatac cacgagcgcg cagtttctcg
     4381 gtaatttcag cgacaggata ttgcgcctgc gcgactgcca tgccgtaccc cggagtaatg
     4441 atcactgaat gggagttttt cagcagttcc gctgtctctt ctgcggtgat ttcgcggtgc
     4501 tcgcccactt cctgatcatc gccagtagaa gagccgtcgg tgccgaaacc acccgcaata
     4561 acgctgataa aggaacggtt catcgcctta cacataatgt aagaaaggat agcgcccgaa
     4621 gaaccgacca gcgcaccggt cacaatcagc aggtcgttgc tgagcataaa gcccgcagcc
     4681 gcagccgccc agccggagta cgagttcagc atcgacacca ccactggcat atctgcacca
     4741 ccgatggagg cgactaaatg ccagccgaat accagcgcaa tggcggtcat tatcagcaat
     4801 gccagcactt gcaggccgac gctatccgtg cgaacaaata caatcagcag caggaaggaa
     4861 acgaccagag ccgccaggtt cattttgtga cggtttggca gcatcaacgg tttagacgaa
     4921 atcttgccac acagtttgcc gaacgccacc accgaacccg tgaacgttac cgccccgatg
     4981 aagataccga ggaacacttc cgtcaggtga atattgacca gaatcggtgc cattcccgcg
     5041 tcatgatgca gataactgtt aaagccaacc agcactgccg ccagacccac gaagctatgc
     5101 aggatcgcca ccagttctgg catttcggtc atttcaactt tcttcgccag acggatacca
     5161 attgccccac caatgaccat cgccagcaag atccagccaa cattacccgt atccggtcca
     5221 aaaatggttg cgattaacgc aatcgccatc ccggcgatac cgaagttgtt accctggcga
     5281 gacgtttcat gtttcgaaag accggccaga ctgaagataa acaggatcgc ggcaacaatg
     5341 tatgcagctg taactaatcc tccagacata tgttacccct taatttttgc ggaacatttt
     5401 cagcatgcgc tgagtcacgg tgaagccacc gaaaatatta atgctggcta taagcaccgc
     5461 gataaaacta aggaagctaa cccagccgcc ctggccaatc tgcaacagtg ctccgacaac
     5521 aataatccct gaaatcgcgt tggtgaccga catcaacggt gtatgcagcg catgcgatac
     5581 attccacacc acgtaataac cgacaacgca ggccagcgcg aaaacggtga agtgcccaag
     5641 aaactctttc ggcgcaacgc ttgccagcca accgaaaagg atgattgcca gcgccatcaa
     5701 tgcgtattta cgccacggtg agcaggcaca tttttcctca gttttcactt ccggtgccgc
     5761 tttttgtgcc gcctgcggct gagctgatac ctgaatcggc ggtgccggcc aggtaatttc
     5821 gcccgcacgg atcacggtca cgccgcgaat caccacatca tcaaaatcaa cagtgatatt
     5881 gccgtctttc tctttgcaca acagtttcag cagattaacg aggttagtac cgtaaagctg
     5941 tgaggattgc gtcggcagac ggcccggaag atcggtataa ccaatcactt tgacaccatt
     6001 ttccgtagtg aagatttcac ccggcacggt gtattcacag ttgccaccgt tttgggctgc
     6061 caggtcgaca atcacactgc ccgccttcat ggagtcaacc atttcacggg taattagctt
     6121 cggcgctggt ttgcctggaa taagcgcggt ggtgacaatg atatcgacct cttttgcctg
     6181 ggcggcaaag agttccattt ccgctttgat gaacgcgtcc gacatcactt tggcatagcc
     6241 atcgccgctg cccgcttcct ctttaaaatc cagctcgagg aattccgcgc ccatactttg
     6301 aacttgttct ttcacttccg ggcgggtgtc gaatgcacgc acaatcgcgc cgagactgtt
     6361 tgctgcgcca atggcggcca gacctgcaac acccgcacca atcaccatca cttttgccgg
     6421 tggcactttc ccggccgcag taatttgccc ggtaaagaag cgcccaaatt catgtgccgc
     6481 ttcaacaatg gcgcgataac cggcgatgtt cgccatcgag cttagtgcgt ccagcgattg
     6541 tgcgcgtgag atacgcggca cagaatccat cgccatcacg gtcacgttac gttccgcaag
     6601 tttttgcatt aattccggat tctgcgcagg ccagataaaa ctcaccagcg ttgtcccagg
     6661 attcagtaac gcaatttcat catctaacgg cgcattgacc ttcagaatga tctctgactg
     6721 ccagacgcta ttcccttcta caatttcagc gcccgcttgc acaaacgctt tatcgtcaaa
     6781 acttgccagt tgacccgcgc cgctctctac cgcgacggta aaacccagtt tcagcagctg
     6841 ttccactgtt tttggcgttg ctgcaacacg ggtttcattg gttaaccgtt ctcttggtat
     6901 gccaattcgc atgatattcc cttccatcgg ttttattgat gatggtttgc ctgtgtcagg
     6961 agccacacaa gccgctcatg tacgagctaa atgttactcc gttaaaataa attagtaaca
     7021 aacgccctat aacgtactga aaattatgcc tgtgatctag cgccaaaaat cagtatttcg
     7081 gcgtgaactc gcaaaatatt aacgattcag ccgtgatagt gggataaaca ccttagaacg
     7141 ccggataaag actgataatt gtcttcgacg gtcgggtaaa acgagacatc gccccggcac
     7201 gaatcactac ttaacattaa attaacttat acaattcagt tgcttcagta gtaatgatgc
     7261 tgatacggct gttttttaag catagacggt catttgagca ggattaaaat tggcttaagg
     7321 aatgtgatat gaaaaatgac gcagacagtt acaccgttta aatgcaataa tcagccacgt
     7381 ttctcgttaa taacaatacc agtacctggt ttgcgcaagg cgaaggatta tttttatgaa
     7441 gcttaagaac accctcctgg cgtcggcact gctttctgct acggcattct ccgttaacgc
     7501 agcaacagaa ctgacaccgg agcaagcggc agcggttaaa ccttttgacc gtgtagtggt
     7561 taccggtcgt tttaatgcta ttggcgaagc ggtgaaagcc gtttctcgtc gcgcagataa
     7621 agaaggtgcc gcctcttttt atgttgtcga cacttctgat tttggtaaca gcggtaactg
     7681 gcgtgtggtc gctgacctct ataaagccga tgctgaaaaa gcagaagaaa caagtaatcg
     7741 cgtaattaac ggtgttgtcg aactgccgaa agatcaggct gttctgattg aaccgtttga
     7801 cacggtcacc gtccagggct tctatcgtag ccagccagaa gtcaatgatg ccatcaccaa
     7861 agcagcaaaa gcgaaaggtg cctactcttt ctacattgtt cgtcaaatcg atgccaacca
     7921 gggcggcaac cagcgtatta ctgcattcat ctataaaaaa gatgctaaga aacgtatcgt
     7981 ccagagcccg gatgtgatcc cggcagattc cgaagcagga cgtgcagctc tggctgccgg
     8041 tggcgaagcc gcgaagaaag ttgagatccc aggtgttgcg actaccgcat caccaagttc
     8101 tgaagtcggt cgtttctttg aaacccagtc atcaaaaggc ggacgttaca ctgtcacgct
     8161 tcctgacggc actaaagtcg aagaactgaa caaagcgaca gcagcgatga tggtcccgtt
     8221 cgacagcatc aaattctctg gcaactacgg caacatgacc gaagtctcct atcaggttgc
     8281 gaaacgtgcc gcgaagaaag gtgctaagta ttaccacatc acccgccagt ggcaggaacg
     8341 tggtaataac ctgaccgtca gcgcagatct gtataaataa ctattcagtt actcctataa
     8401 ggcggcttga tgccgccttt cgcattttta gtcacaaatt tccccgcgac cattgcatgc
     8461 agacacatct cttcgtaaaa tcccgcgctt cagtggcaaa ctccattttt atgcgttttg
     8521 ccctaatatt tattcttctt atcacgtttt aatcactgga tatcgatgga aaagaaactg
     8581 ggactgagcg cactcaccgc gctggtatta agctcaatgc tgggcgcggg tgttttcagt
     8641 ctgccgcaaa atatggcggc agttgccagc ccggcagcac tgctcatcgg ctggggtatt
     8701 actggcgctg gcattttatt gctggccttt gccatgctga tcctcacgcg cattcgtccc
     8761 gaacttgacg gcggtatctt tacctatgcc cgcgaagggt ttggcgagct aatcggcttt
     8821 tgttccgcat ggggatactg gctgtgcgca gtcatcgcca acgtctccta tctggtgatc
     8881 gttttttccg cgttaagctt ttttacggac acgccggaat tgcgcctgtt cggcgatggt
     8941 aatacctggc aatcgatagt cggtgcatcg gctttattgt ggattgttca ctttttgatc
     9001 ctgcgcgggg tgcaaaccgc tgccagcatt aacctggtgg caacactggc aaaattgttg
     9061 ccgctgggtc tgtttgttgt gctggcgatg atgatgttca aactggatac cttcaagctc
     9121 gacttcaccg gacttgcact tggcgtaccc gtgtgggaac aggtgaaaaa caccatgctg
     9181 atcaccctgt gggttttcat tggtgtggaa ggtgccgtgg ttgtttctgc gcgtgcgcgt
     9241 aataaacgtg atgttggcaa agcgacactg ctggcggttc tctccgctct gggcgtttac
     9301 ttgctggtaa cgctgctttc gctgggcgtg gtggcacgtc ctgaactggc tgaaattcgt
     9361 aacccgtcaa tggccggtct gatggtggaa atgatgggac catggggcga aatcatcatc
     9421 gctgccggtt tgatcgtttc cgtttgcggc gcgtacttaa gctggaccat catggcggca
     9481 gaagttccgt tcctggctgc cactcataaa gcattcccac gcatttttgc gcgccagaac
     9541 gcacaagctg cgccatccgc ctcgctgtgg ttgaccaaca tctgcgtgca aatttgtctg
     9601 gtactcatct ggctgaccgg ttccgattac aacacgttgc tgaccatcgc ctcagaaatg
     9661 attctggtgc cctatttcct ggtcggcgcg ttcttgctga aaatcgcaac ccgtccactg
     9721 cataaagcgg taggtgtcgg tgcctgcatt tatggcttat ggttattgta tgcctctggt
     9781 ccaatgcact tgctgttatc cgttgttctg tatgcgccgg ggctgctggt tttcctgtat
     9841 gcgcgcaaaa cgcatacaca tgataatgta ctgaaccgtc aggaaatggt gctgattggc
     9901 atgctgttaa ttgcctcagt tccggcgacc tggatgctgg tgggataacg ttcatcccat
     9961 cgttggatag caaaggagat aacgatgggt aaagcccagc ccttgccaat attaattact
    10021 ggcggaggtc gtcgcatcgg cctcgccctc gcctggcatt tcattaatca aaagcaaccg
    10081 gtgattgtca gctatcggac gcactatcca gccattgatg gactgattaa ggcaggtgcg
    10141 cagtgtattc aggctgattt ttcgaccaac gacggtgtga tggcgtttgc cgatgaagta
    10201 ctaaaaagca cccatggtct gcgtgctatt ttgcataacg ccagtgcgtg gatggcggaa
    10261 aaaccaggta caccactggc cgacgtactg gcttgcatga tgcagatcca cgttaatacc
    10321 ccatacctgc tcaaccatgc gctggaaaga ttactgcgtg ggcacggaca cgccgccagc
    10381 gatatcattc actttaccga ttatgtggtg gagcgcggta gcgacaaaca tgttgcgtat
    10441 gctgcaagca aagcggcact ggataatatg acccgctcgt ttgcccgcaa gctggcaccg
    10501 gaagtgaaag tgaattctat tgcgccatcg ctgatcctgt ttaatgaaca tgatgatgcc
    10561 gaatatcgac aacaggcgct gaataaatca ctgatgaaaa ccgcgcctgg cgagaaagaa
    10621 gtgatcgacc tggtcgatta cttacttacc agctgctttg tcaccggacg cagtttccca
    10681 cttgatggcg gtcgtcatct gcgttaatgc agctttatcc agcatataat caacacccat
    10741 gcgcttatcc cccaacacgc aacagaacca acaaaagcgg cgggcaagcg catcattccg
    10801 gtgaaatacc acagcgacac caggtaaaca aaatagggaa taatcgacca catactaaaa
    10861 atgatggttg cgcgtaaggc ttcaatgccg cgttcgctgg caacaatata atgcgcgata
    10921 agcgcaaagg tcgggaaaag tggaatcagc ccggcgatat aataattttt cgtttttgct
    10981 aaaacaccaa tcaacagcac taccagcgca ccgagcgcgg ctttgattac cagccccatc
    11041 tttttacctt aacacttcca taacaagtca tcagtagaat acctgatgaa aacttgttta
    11101 gaaacgattg atagtaagta aaaacagcgc ggtgtattgt gacgttttta tatctaccgt
    11161 gaatgttatg aacactatcg tatttgtgga agatgatgcg gaagtcggtt cactgattgc
    11221 cgcgtacctg gcaaaacatg atatgcaggt taccgtagaa ccgcgcggcg accaggccga
    11281 agaaaccatt ttgcgagaaa gtccggattt ggtgttactc gacatcatgc taccaggcaa
    11341 ggacggcatg accatttgtc gtgatttacg cgcaaagtgg tctggaccga ttgttcttct
    11401 aacctctctc gatagcgata tgaaccacat cctggcactg gaaatgggtg cctgcgacta
    11461 tattctcaaa acgacgcccc ctgctgtttt gctagcgcgt ttacgtttgc atttgcgtca
    11521 gaatgagcaa gccacactga ccaaaggtct tcaggaaacg tctctgactc cctacaaagc
    11581 cctgcatttc ggcacgttga ccatcgatcc catcaaccgc gtagtcaccc tggctaacac
    11641 tgaaatctcg ctctcgacag ctgatttcga attattgtgg gaattagcta cccatgccgg
    11701 gcaaatcatg gaccgcgatg cattgctgaa aaatttacgc ggcgtcagtt atgacggact
    11761 ggatcgtagc gtggacgtgg ctatttcgcg gttaagaaaa aaactgctcg ataacgccgc
    11821 agaaccttat cgcattaaaa ctgtgcgtaa caaaggctat ctttttgcgc ctcatgcatg
    11881 ggaataagcg atgaaaaaac tgtttatcca gttttacctg ttattgtttg tctgcttcct
    11941 tgtgatgtct ctgctggttg ggttggtgta caaatttacc gccgaacgcg cgggcaaaca
    12001 gtcgctggat gatttgatga acagttcgct gtatctgatg cgcagcgaat tgcgtgagat
    12061 ccccccacac gactggggta agacgctgaa agagatggat ttaaatctct ctttcgatct
    12121 gcgtgtcgag ccactgagta aataccatct tgatgatatt tccatgcacc gactgcgtgg
    12181 cggcgaaatt gtcgccctgg acgatcagta cacgtttttg cagcgtatcc cgcgcagcca
    12241 ctacgtgctg gcagttggtc ctgttcctta tctttattat ctccatcaga tgcgattgct
    12301 ggatatcgcc ctgatcgctt ttattgctat ttccctcgcc tttccggtgt ttatctggat
    12361 gcgtccgcac tggcaggata tgttaaaact ggaagcagcg gcgcaacgat ttggcgatgg
    12421 gcatctcaat gagcgtatcc actttgatga gggttcgagc tttgaacgac ttggtatcgc
    12481 atttaaccag atggcggaca atatcaacgc cttgattgcc agcaaaaaac agcttattga
    12541 cggtatcgct cacgaactgc gaacaccgtt agtgcgcctg cgttatcgac tggagatgag
    12601 cgataacctg agcgccgccg aatcccaggc gttgaatcgt gatatcagtc aacttgaagc
    12661 tttaattgaa gagctgctga cttatgcccg actcgatcgc ccacaaaacg agcttcatct
    12721 tagcgaacca gacctgccgt tgtggctgtc aacgcatctg gcagatattc aggcagtaac
    12781 gcccgataaa acggtacgga taaaaacgct cgtgcaaggc cattatgcgg cgttggatat
    12841 gcgcttaatg gagcgcgtgc tggataattt gctcaataac gccctgcggt actgccattc
    12901 aacggttgaa accagcctgc tactgtcggg gaatagagcg acattaattg ttgaggatga
    12961 tggcccaggg attgccccag aaaaccgcga acatatcttt gaaccttttg ttcgcctcga
    13021 tcccagccgg gatcgctcaa ccggcggctg cgggctgggg ctggcaattg tccactctat
    13081 agcactggca atgggcggta cggttaattg tgacaccagc gaactgggtg gtgctcgctt
    13141 ctcgtttagc tggccgttat ggcataacat cccgcaattt acctctgcct gacactacgc
    13201 gcacgatggt caagtcacca cgactgtgct ataaaataag tatgttgtaa ctaaagtggt
    13261 taatattatg gcgcgttacg atctcgtaga ccgactcaac actacctttc gccagatgga
    13321 acaagagctg gctacatttg ccgctcatct tgagcaacac aagctattgg ttgcccgcgt
    13381 gttctctttg ccggaggtaa aaaaagagga tgagcataat ccgcttaatc gtattgaggt
    13441 aaaacaacat ctcggcaacg acgcgcagtc gctggcgttg cgtcatttcc gccatttatt
    13501 tattcaacaa cagtccgaaa atcgcagcag caaggccgct gtccgtctgc ctggcgtgtt
    13561 gtgttaccag gtcgataacc tttcgcaagc agcgttggtc agtcatattc agcacatcaa
    13621 taaactcaag accacgttcg agcatatcgt cacggttgaa tcagaactcc ccaccgcggc
    13681 acgttttgaa tgggtgcatc gtcatttgcc ggggctgatc acccttaatg cttaccgcac
    13741 gctcaccgtt ctgcacgacc ccgccacttt acgctttggt tgggctaata aacatatcat
    13801 taagaatttg catcgtgatg aagtcctggc acagctggaa aaaagcctga aatcaccacg
    13861 cagtgtcgca ccgtggacgc gcgaggagtg gcaaagaaaa ctggagcgag agtatcagga
    13921 tatcgctgcc ctgccacaga acgcgaagtt aaaaatcaaa cgtccggtga aggtgcagcc
    13981 gattgcccgc gtctggtaca aaggagatca aaaacaagtc caacacgcct gccctacacc
    14041 actgattgca ctgattaatc gggataatgg cgcgggcgtg ccggacgttg gtgagttgtt
    14101 aaattacgat gccgacaatg tgcagcaccg ttataaacca caggcgcagc cgcttcgttt
    14161 gatcattcca cggctgcacc tgtatgttgc agattaacgc ccggctttca tactgccgac
    14221 catctgttct ggccgtaccc agctgtcaaa ctcggcttcg ctaagatacc ccagcgcaag
    14281 ggccgcagct tttaaggtca gcccttcttt atgcgctttt ttggcgatct ccgcggcttt
    14341 gtcataacca atgtgggtgt taagcgcagt caccagcatc agcgattcat tgagtaattg
    14401 attgattcgc tcacgattcg gttcaatacc cactgcgcag tgtttgttaa aactttccat
    14461 gccatctgcc agcaagcgca ccgattgcag gaaattgtgg atcaccattg gacggaagac
    14521 gttcagttca aagttaccgg aagcgccccc catgttgatc gccacgtcgt tccccatcac
    14581 ctgacagcag agcatggtta atgcctcaca ctgtgttgga ttcactttcc ccggcatgat
    14641 tgagctgccc ggctcatttt ccgggattga gatttcacca attccgcagc gcgggccaga
    14701 ggccagccag cggacatcat tggcgatttt catcagtgac acagccaacc ctttcaacgc
    14761 gccgtgcgcc tgaaccaggg catcacaggt cgccagcgct tcaaatttgt tcggcgcggt
    14821 aacaaacggt gcacaggtaa tgactgccag ttcatctgct acgcgacgcg catactccgg
    14881 atgggtattt agtccagtac ccaccgctgt accgccaaga gccagttccg ctacgtgagg
    14941 caggctgtat tcgatatgtt tgagattatg ctcgagcatc gctacccagc cggaaatctc
    15001 ctgccctagt gttagcggcg tggcgtcctg caagtgggtt cgaccgattt tgacgatatc
    15061 ggcaaatgca cgcgattttt cactcagtgt ctgtgtcagg gttttaagct gcggaatgag
    15121 ttgcttgcgc agcgccagca gcgccgcaac gtgcatcgcc gtcggaaaga catcgttgga
    15181 actttggctt ttgttcacgt cgtcgttagg gtgaacttta cgttccatcc cgcgcacgcc
    15241 gccgagtaat tcactggccc ggttagccag cacttcgttc atgttcatgt tactttgcgt
    15301 gccggagccg gtctgccaga tagccagcgg gaattcgtcg tcatgctgtc ctgccagtac
    15361 ttcatccgcc gcctgccgaa tggcgctcgc tttctcttca gacaacaagc ctaaatcttc
    15421 attaactttt gccgctgcgc gcttggttag cgccagcgca tgaatcagtg aggtgggcat
    15481 tttctccgtc gaaatgcgga aatgctccag cgagcgttga gtttgtgcgc cccacagctt
    15541 atctgccggg acatcaatcg cccccatcga atctttttcg ctgcgtactg tattcatggc
    15601 ctgctcctca cctgtttaat tttttctttc tgttttgctt tcgttaagca acttttagct
    15661 cacttattat ttactatttg ataacaaatg tttggtcttt cgtgccatgt aaaaaaaccg
    15721 ccccgaaggg cggctctgtt tatttcacac agcgagtgca ctgtgtgagt tgtatctgct
    15781 ggaagaagtc atttccttta tcatccacaa ggataaacgc cgggaaatct tccacttcaa
    15841 ttttccagat ggcttccatt cccagttccg gatattcaac acattccaag ctcttaatac
    15901 ttccctgcgc caatacagcg gccggaccac cgatactgcc aaggtagaag ccgccgtgtt
    15961 ttttacaggc atccgtcacc tgctggctgc ggttgccttt cgccagcatg atcatacttc
    16021 cgccctgcgc ttgcagttga tcgacataag aatccatccg tccggcggtc gttgggccaa
    16081 gagaaccgga ggcataacct tccggcgttt tggccggacc cgcgtagtaa atcggatgat
    16141 ctttaatgta ctgcggcagc ccttcaccgt tatccatccg ctctttcagt ttggcgtgag
    16201 caatatcacg accgacgata atcgtgccgt taagcgataa gcgtgtagaa acgggatact
    16261 gcgacaactg tgcgaggatc tctttcatcg gacggttaag gtcaacgcgc accgcttcgc
    16321 cttctcccgc tttacgcagc tcttcaggga tatatttacc tggattatgt tccagttttt
    16381 cgatccagat cccctgacgg ttgatcttcg ctttgatatt acggtcagca gagcaggaaa
    16441 cacccatacc aaccggacag gatgcgccgt gacgtggcag gcgaatcacg cgtatgtcgt
    16501 gagcgaagta tttaccacca aactgcgcac ccagaccgag attttgcgct tcgatcagca
    16561 attctttttc cagttccaca tcgcggaatg cctgaccgtg ctcattccct tccgttggca
    16621 gttcgtcata gtatttagcg gaagccagtt tcactgtttt aaggttagtt tctgcagaag
    16681 ttccaccaat tacgaacgca atatgatacg gaggacaggc cgccgtaccc agcgtgcgca
    16741 tcttctcaac caggtaattt ttcagtttcc ccggcgttag taacgctttg gtttcctgat
    16801 agagatacgt cttgtttgcc gaaccaccac ctttggcgat acagaggaat ttgtactcgt
    16861 cgccatcaac ggcataaaga tcgatctgcg ctggcagatt ggtgccggta ttcacctctt
    16921 tatacatatc cagcggcgcg ttttgcgagt agcgcaaatt atcttcgata taagtgttat
    16981 agacaccgcg cgccagcgcc gcttcatcac agccaccggt ccatacacgc tgcccttttt
    17041 taccaacaat aatcgcagtg ccggtatcct gacaggttgg cagaacgcct ttcgccgcga
    17101 tgtcggagtt acgcaggaat tgcagcgcca catatttatc attttcgctg gcctccgggt
    17161 cacgcagaat gtcggccacc tgttgttggt gagccggacg cagcataaac gacgcatcat
    17221 gaaacgcctg acgcgccaac agagttaacg cttcgggtgc gactttcaaa atctcctgcc
    17281 cttcaaattc agatacgcta acgtgttcgc tggttagcag gtaatactca gtatcatctt
    17341 ttttgagtgg aaaaggagcc tgataatgaa agggtttgtt tgacattgtt ctctcactta
    17401 ctgcctggtt tggttatgct ctgggcgggt gttccgttgc cctgttaaaa gcgagtaaca
    17461 atatcctaca cactttttta acaaaaactg agactagtac gactttttgc ggctccaggt
    17521 tacttcccgt aggattcttg ctttaatagt gggattaatt tccacattaa aacagggatt
    17581 gatcatgcaa aaactcatta actcagtgca aaactatgcc tggggcagca aaacggcgtt
    17641 gactgaactt tatggtatgg aaaatccgtc cagccagccg atggccgagc tgtggatggg
    17701 cgcacatccg aaaagcagtt cacgagtgca gaatgccgcc ggagatatcg tttcactgcg
    17761 tgatgtgatt gagagtgata aatcgactct gctcggagag gccgttgcca aacgctttgg
    17821 cgaactgcct ttcctgttca aagtattatg cgcagcacag ccgctctcca ttcaggttca
    17881 tccaaacaaa cgcaattctg aaatcggttt tgccaaagaa aatgccgcag gtatcccgat
    17941 ggatgccgcc gagcgtaact ataaagatcc taaccacaag ccggagctgg tttttgcgct
    18001 gacgcctttc cttgcgatga acgcgtttcg tgagttttcc gagattgtct ccctactcca
    18061 gccagtcgca ggtgcacatc cggcaattgc tcacttttta caacagccta atgccgaacg
    18121 tttaagcgaa ctgttcgcca gcctgttgaa tatgcagggt gaagaaaaat cccgcgcgct
    18181 ggcgatttta aaatcggcgc tcgacagtca gcagggtgaa ccgtggcaaa cgattcgttt
    18241 aatttctgaa ttttacccgg aagacagcgg cctgttctcc ccgctgttac tgaatgtggt
    18301 gaaattgaat cctggcgaag cgatgttcct gttcgctgaa acaccgcacg cttacctgca
    18361 aggcgtggcg ctggaagtga tggcgaactc cgataacgtg ctgcgtgcgg gtctgacgcc
    18421 taagtacatt gatattccgg aactggttgc caatgtgaaa ttcgaagcca aaccggctaa
    18481 ccagttgttg acccagccgg tgaaacaagg tgcagaactg gacttcccga ttccagtgga
    18541 tgattttgcc ttctcgctgc atgaccttag tgataaagaa accaccatta gccagcagag
    18601 tgccgccatt ttgttctgcg tcgaaggcga tgcaacgttg tggaaaggtt ctcagcagtt
    18661 acagcttaaa ccgggtgaat cagcgtttat tgccgccaac gaatcaccgg tgactgtcaa
    18721 aggccacggc cgtttagcgc gtgtttacaa caagctgtaa gagcttactg aaaaaattaa
    18781 catctcttgc taagcttatt aaaggcttat aacaccttca ggcggccagt ccgcctgatt
    18841 tcattttatg gataatcatt atgaataaat cgctggtagc ggtaggcgtc attgttgcgc
    18901 taggcgtagt ctggacaggc ggcgcatggt atacaggcaa gaagattgaa acccatctcg
    18961 aagacatggt cgcgcaggcg aacgcgcaac tcaaactgac agctcctgaa tccaacctgg
    19021 aagtgagtta tcaaaactat catcgcggcg tattcagcag ccagttgcaa ctgttggtga
    19081 aacccattgc cgggaaagaa aatccgtgga ttaaaagcgg tcagagcgtc atcttcaacg
    19141 aatcggttga tcatggtccc ttcccgcttg cccagcttaa aaaactgaac ctgatcccgt
    19201 cgatggcatc aattcaaacc acgctggtta ataacgaagt aagcaaacca ctgtttgata
    19261 tggcaaaagg tgaaacgcct tttgagatta actcgcgcat tggttacagc ggtgattcca
    19321 gttccgatat ttcgctcaag ccactgaatt acgagcaaaa ggatgaaaaa gtcgccttta
    19381 gcggcggcga gttccagtta aatgctgaca gagacggcaa agccatctcc ctttccgggg
    19441 aggcgcaaag tggtcggata gacgcagtta acgaatacaa ccagaaagtg cagttgacct
    19501 ttaataatct gaaaaccgac ggttccagca cgctggcaag ttttggtgag cgcgtaggaa
    19561 accaaaaact gtcactggaa aaaatgacca tttcagtgga aggcaaagaa ctggcactgc
    19621 tggaaggcat ggagatcagc ggtaaatcgg atctggtcaa tgacggtaaa acgatcaata
    19681 gccaactgga ttactcgcta aacagcctga aggtacagaa tcaggatctg ggcagcggca
    19741 agctgacttt aaaagtcggc cagattgatg gtgaagcctg gcatcagttt agccagcaat
    19801 ataacgcgca aactcaggcg ctgctggcac agccagaaat tgccaacaat cccgaacttt
    19861 atcaggagaa agtgacggaa gccttcttta gcgccctgcc gctgatgttg aaaggcgatc
    19921 cggtgattac tatcgcgccg ctaagctgga aaaacagtca gggtgaaagt gcgctgaatc
    19981 tgtcgctgtt cctgaaagat ccggcaacga ctaaagaagc gccgcaaacg ctggcgcagg
    20041 aagtagatcg ttcggttaaa tctctggatg cgaaactgac cattccggtg gatatggcaa
    20101 ctgagtttat gactcaggta gcgaagctgg aaggttatca ggaagatcaa gcgaaaaaac
    20161 tggcgaaaca gcaagttgaa ggtgcatcag caatggggca gatgttccgt ctgaccacct
    20221 tgcaggacaa taccatcacc accagcctgc aatatactaa cggtcagata acgttaaacg
    20281 ggcagaaaat gccactggaa gatttcgttg gtatgtttgc aatgccggca ttaaatgttc
    20341 cggtcgtacc cgctattccg cagcagtaat tcacagacaa aacgccggag atttttctct
    20401 ccggcgttat tttttacttc agcataaagt catactgaat actcattttc acatcccagt
    20461 aattcaggcg atcgcccagc gtactgccat cgcgcttttc ccgcccttta tcggcagcaa
    20521 gaaagaaatt catcccttgt aacggccctt cgctgacccg atagttcata ccacctaaga
    20581 tacccgtgcc ttcaatccgc acatctcgtt tactggattc atagccgcga gtcacaatcg
    20641 gtgcaaatgt aagcgtaaat tgcggcgtta aacgatagtt gacgcccaat tgccaggact
    20701 gcattccttc acggttacga tcaagactta atgaaaaggg atagccaatg tcggtatcca
    20761 ccacggcacc attcattgca tggcgcaaat aaccaccact gccaaatacg cttccccacg
    20821 gcgcattcca ggtcaattgt ccactaacca acgccgtttc attgggctgg ctggtattac
    20881 gacttaaacc ttcgagctgc gcgtaaaagc cgagcaactc ccctttcaac gtataattcg
    20941 ggtcgagcgg tatgcttttc tcgagaatac tttgtatctg attggaaaca tctttctgta
    21001 ccgatgcccc cagagaatag cgactggtat catcatgcca actggcggct aaaaaagaac
    21061 gtgcgcgatg tttatcgtat tttcccgtct ggctgttata caccccttca tcacgaatac
    21121 cggttgttga atcagtccat aaaaatactc gtgaacgcca cattccccac agatcaatat
    21181 ttggtgttaa agcccaataa cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg
    21241 aactgccagc cattaacgga atacgataag gtaagctacc ataactataa tccgtcccga
    21301 agcgacctaa tttaagcagg ctattttcac tgggttttat ttttactgcc cccagggcaa
    21361 agccaaaact tttatcacca tcaagatacc accaggtact catatcagca cgagcaccta
    21421 atttataaac ataatatgcc ccgccttcaa cgccaataat attggaataa taaccactat
    21481 tgaaatcgag caatcctccc tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg
    21541 gcttatccgc cctgcgcaat tcgtttttca gtctgatgcg tagtcccgct tcatcgtcag
    21601 ccagttgcgc cgcataagca gaggtaaggc cagaggcagc cgtcaggcaa ataacggcca
    21661 tggccactat ttttctcata gttgcacctt tgatgttctg attattttta ttgaatatta
    21721 attagtgata tcgctgatta attgctgctg cactttttta cgattatcaa tttcaaccac
    21781 gatttctttg aattttttat ccgtgagcgg ataaaaccag ataataacga atgccagtag
    21841 cataaatccg caaggtacta aggcaattga tgtgcggatg cccataataa cttccggcgt
    21901 ttgcacctga ttggcgatat atccgcttaa tcccaaaata aaggcaggaa ttgaacctcc
    21961 gattgcctga ccgcatttac gggtaaataa gaatagtgaa taggtgagcc cttcaattcg
    22021 cacgccggtc aggtattcac cgtattctac ggtatcagct tccagtgccc acatcacagt
    22081 catagtaacg ccctgaccaa ttgaagcgat ggccaacgca accaacgcca ccggcagtga
    22141 ccagacggaa acccagaaga atagcagata accgcaggtt cccagcaaag cgccaatcag
    22201 gaaggtattc tttttaccga tcctcgcgac catccccggc accagcggtg ccgatgccac
    22261 agtaccaacc aggttttgca ccagtaccag cacagtgaac agcccggtat catttaacac
    22321 atagcgcacg tagaacaacg acgaggcgct gaccgcgaag gtcgaaatca gcacacacag
    22381 cgcaccgatg cacaacataa acagcgggcg attccgtttc agggtttgca gactgatctt
    22441 caatgacggc tgcgccacga tacgtaccac attctcacgc gtcgatttga agcagatgaa
    22501 gtaaagcacc attccggcaa tcgccagcac aatcgtccag aaatggtata ccgacaccat
    22561 ctcttccggg ctggagttct taatgctcgg tcctatcaga aatgccaggc agacaaaggt
    22621 taatgaagcg gcaatcccgc gagccgcgcc cagacgggcg cgggattgtg gttgttgggt
    22681 catcgcggta gcaagtgaac cataaggaat attaaccagg ctgtagcaaa gcccgaggcc
    22741 catgtaggtc aaatatgcat acaccacttt gctaccatgg ctccagtcgg tcggcaccca
    22801 gaataccagc acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa
    22861 ttttccccag cgggtattca cactgtccac cactcgtccg gcaaagacgt cggcgaaggc
    22921 atcgaatacc cgcaccagta acagcatggt gcccgccgca gcggcaccga cgccagcgac
    22981 gtcggtgtag taactcaata ggaagagcgc ccccattgcg aaggcgaagt tattggcgac
    23041 gtcaccgagg ctgtagccga cgatggtgcg ccaggagagt tgttgattca ttgtttgcct
    23101 ccctgctgcg gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc
    23161 gccgacttcg gtttgcggtc gcgagtgaag atccctttct tgttaccgcc aacgcgcaat
    23221 atgccttgcg aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg
    23281 acgcgatcaa agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac
    23341 atgtcggtgt acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata
    23401 atcggctgat gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc
    23461 gtttccaaat cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca
    23521 aagagatcgc tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc
    23581 ggacgcgtcg ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca
    23641 ccttgcggac gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt
    23701 ttgtcacgcg ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg
    23761 actgcctctt cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa
    23821 gagaggttaa agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc
    23881 cagtcgagca tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc
    23941 cagtccatta atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag
    24001 tccgcatctt catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac
    24061 tgttcgccct tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct
    24121 gtctggcttt tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg
    24181 tgcggattca ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttgc
    24241 tccgcatcac gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac
    24301 gcgtggttac agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc
    24361 ggcgtggtgt agagcattac gctgcgatgg atcccggcat agttaaagaa atcatggaag
    24421 taagactgct ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc
    24481 cagttcagtt cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac
    24541 ggcgtgacat cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta
    24601 ttgacccaca ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct
    24661 gcccaacctt tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga
    24721 atatctgcat cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct
    24781 tgtaacgcgc tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat
    24841 gcccacaggc cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacata
    24901 agggactcct cattaagata aaaatactgg tcaaccttta atctgattag ttaaccaatt
    24961 ctgtgttgca cacagttatc tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac
    25021 tgacaccgcg atcaaaaaac caaaaactgc aatacccaca gcgtgatatt gatcgcatta
    25081 atctttaaaa cggcgttgac agaagccata aaataagcaa ccgaacgttc gttcgagcat
    25141 attgggaacg ataaactctc atgacatatg gctacggtga atatttttgg gggggaattc
    25201 tccgatcccg gctagattgt attttgtaca gggaaattaa agtatgaaac gcgggcacag
    25261 ttatcaccag cccgcgaaat gagaggatca ggatgcggtt aagataccgc caatcatcgc
    25321 gcgtaatccc tgagcaaacg acgcttcacg cgccagatct tccgcttcga tatcggccag
    25381 tacaccgtag gtcagatcca gcagtaaacg tgaagtcatt gacgtgttga tgtctgggct
    25441 tatctcgcct ttttgctggg catcacgcat ccgctcggca acaaattcag tgatcgtcat
    25501 atgcttattt ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc
    25561 gaccaccagc gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat
    25621 atagtcaaca aaatgaatcc cttcaatcgg ttcccggaaa cgggccagcg ccctctcctg
    25681 gtcctgtaag ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt
    25741 cccgggacta atggcgcagg atttacagat ggctttcatc gaggcactgt gaaatccatt
    25801 ttctgaaaaa atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt
    25861 ctgcatgtta tccatcataa aaccattata gttagcgtga ataactagtt taccgcgttg
    25921 tcaggtgagc gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatactt
    25981 ttcactctga aattggttaa ccaatagccg tgatttggct gccagttatt tagcagagac
    26041 gttgatgagt ttatcccggt aaacaacacg cacggttgtt taccggtccg ttagtgtatt
    26101 aattgagctc ctgtacccca ccaccggaga cggtgagaat ttgtccgctt acccagctcg
    26161 cagcaggcga gcaaaggaac agcgctgcgt tagcaatatc ttgcggttgg cccagacgtc
    26221 tgatcggcgt gtgctgtaac attttttgtt caatttctgg tgtaataacg gatttcaggg
    26281 catcggttaa tattgccccc ggcgcaatgc catttacccg aatatttttt tcacccaggt
    26341 caaacgccat atttctgacc agatgactgg ctgcagcttt agatgatgca taggaagtca
    26401 tgtttatatt tttattttct gccgccatag aagtgatggt cagaataacg ccaccgccat
    26461 ttttttccat ttctggcgca acaagttgtg acagatggaa aaaagaaaac acattcagtt
    26521 cataagcacg gcgaaaatcc gccattggca tatcaaacgg tttaggtcca ccgccaccgg
    26581 cgttgttaac cagaatatca actttaccca gcttactgat agcaaagtct gccagtgcag
    26641 agagttcctg ttcggaagta atatcacaac ggcaggcaaa tgcctgacca cccagttgtt
    26701 gaatttcgtc tacaacatgg ttagctgcgt cggcgttaat atcactgacc accacagatg
    26761 cgccagctgt cgcgaatgta atggcaattt ctttaccaat acctgcaccc gcacctgtga
    26821 tgatggcgca ttttccgtcg agtctcaggt tgtcagaatt aaacacataa acctcctgtg
    26881 aacttcagta aaaataaaag gcaccgcctg tgtgccacat tttttatcat taaccttgag
    26941 gaaaggacac taccaggtat agcagataaa gagaggatta tttcgccgca atgagccggg
    27001 cgggaataat aagattgcgt gaatgcgtct cttcatgggt gattttttgc atcatgcgat
    27061 ccgcaagtgt gataccaagt tcccgcgctg gcgtgctggc ccaggtaaca ggtatatcat
    27121 caagtgtggt tggtgtcgca tcggtaaatg ccgccagcga aacctgttgc tcaaagtaac
    27181 gatcgactcc gctttccccg ctttgccgcc ctgctttgag caaaccaaac catgccccca
    27241 tcgcaatagt ttcgttatag caaaccacgg cactgatggt cgggttatga cgtaaaagcg
    27301 ccgtgatagc ttccgcggct tgcttctggc tggaagtgca ctccaacacc caatcgctat
    27361 gaaacggcag gccaaatttt agtagagttg cacaatagcc ccccacccgt tctgcacggg
    27421 ttaatgagga actttgccct cccagccagg cgatccgctg atgcccattg cgaatgagat
    27481 gctccgtcaa caactgtgca gcctgcatgt tgtccgggcg aaccgtatca acatcatcaa
    27541 gataactggc acgggaagcg aaaatcaccg ggatagcttt ttcttctgcc atccgtcgca
    27601 ggtcatcgct gcttcctgca gccccggcaa ttaccacacc atccacaccc tgattcagta
    27661 acagtgaaaa ccgctgtgcc agctgctcgc cgtctttacc gccgtgaagc aaaaaaacca
    27721 tccgtccctg cgcttccaga gcttccgtca atccggccgt caattcggcg taaaacggcg
    27781 cagataaatc acggacgatc aaaccaatga cgccgctttg cccgccgcgc agcgccgacg
    27841 cctggcgatt gcgcacaaat cccagctctt caatggcggc gttaacgcgt tctcctgtgg
    27901 cggtagagat tcgccctttg ccactaagca ccagcgaaac ggtacttacc gacacgcccg
    27961 cagccagcgc aacatcatga atggttattt ttttggcggt agccatgcag atgacctact
    28021 ccctgattat gtgacagata aaacgtttta ccttttattt atcttatacc cgctattatc
    28081 gttgcgtaat gtgatttatg cctcactaaa atttgataaa acgttttatc ttctcgcaca
    28141 atttactgaa tccagattgt tctctacgag gagtcgtttt atgacggcga aaacagcacc
    28201 gaaagtcacg ctgtgggagt tcttccagca gttaggcaaa accttcatgt tacccgtggc
    28261 attattgtcg ttctgcggca ttatgctcgg cattggtagt tctcttagta gccacgatgt
    28321 catcaccctg atcccggtcc tgggcaaccc cgtgttgcag gctatcttta cctggatgag
    28381 taagattggc tcgtttgctt ttagtttcct gccagtcatg ttctgtatcg ccatcccgct
    28441 gggtctggca cgcgaaaaca aaggcgtagc ggcattcgct ggcttcgtcg gttatgcggt
    28501 aatgaacctc gcggtaaact tctggttgac caataaaggc attctgccaa ccacggacgc
    28561 cgcggttctg aaagccaata acatccagag cattcttggg atccagtcga tcgacaccgg
    28621 tatcctcggt gcggtgatcg ccggtattat cgtctggatg ctgcatgagc gattccataa
    28681 tatccgcctg ccggatgcgc ttgcattctt cggcggtacg cgcttcgtac caatcatctc
    28741 ctcgctggtg atgggccttg tcggcctggt gattccatta gtctggccga ttttcgcgat
    28801 gggtattagc ggcttgggcc atatgataaa cagcgcgggt gatttcggac cgatgctgtt
    28861 tggtaccggt gaacgtctgc tgttgccgtt tggtctgcat cacattctgg tggcattaat
    28921 tcgctttacc gacgcaggcg gcacgcagga agtctgcggt caaaccgtca gcggcgcatt
    28981 gaccatcttc caggcgcaat tgagttgccc gaccactcac ggtttttctg aaagcgccac
    29041 gcgtttcctt tcgcaaggta aaatgcctgc gtttctcggc ggtctgccag gtgcagcgtt
    29101 agctatgtat cactgcgcgc gcccggaaaa tcgccataaa attaaaggtc tgctgatttc
    29161 tggcctgatc gcctgcgtcg ttggcggcac taccgaaccg ctggaattcc tgttcctgtt
    29221 cgtagcgcca gttctgtatg tcatccacgc gctgttaacc ggcctcggct tcaccgtcat
    29281 gtctgtgctc ggcgtcacca tcggtaatac cgacggcaat atcatcgact tcgtggtgtt
    29341 cggtattttg catggtctgt caaccaagtg gtacatggtg ccagtggtgg cggcaatctg
    29401 gtttgtcgtt tactacgtca tcttccgttt cgctatcacc cgcttcaatc tgaaaacccc
    29461 ggggcgcgat agcgaagttg ccagctcaat cgaaaaagcc gttgccggtg cgccgggtaa
    29521 atcaggttac aacgttcctg caatcctcga agcattaggc ggtgccgaca atattgtcag
    29581 cctcgataac tgcattaccc gtctgcgttt gtctgtgaaa gatatgtcgc ttgttaatgt
    29641 gctggcactg aaggacaatc gggcaattgg cgtagtacaa cttaatcaac ataacctgca
    29701 ggttgttatc gggccacaag ttcagtcagt aaaagatgaa atggccggtc tgatgcatac
    29761 tgtccaggca taaggataag atatgttcga tttttcaaag gtcgtggatc gtcatggcac
    29821 atggtgtaca cagtgggatt atgtcgctga ccgtttcggc actgctgacc tgttaccgtt
    29881 cacgatttca gacatggatt ttgccactgc cccctgcatt atcgaggcgc tgaatcagcg
    29941 cctgatgcac ggcgtatttg gctacagccg ctggaaaaac gatgagtttc tcgcggctat
    30001 tgcccactgg ttttccaccc agcattacac cgccatcgat tctcagacgg tggtgtatgg
    30061 cccttctgtc atctatatgg tttcagaact gattcgtcag tggtctgaaa caggtgaagg
    30121 cgtggtgatc cacacacccg cctatgacgc attttacaag gccattgaag gtaaccagcg
    30181 cacagtaatg cccgttgctt tagagaagca ggctgatggt tggttttgcg atatgggcaa
    30241 gttggaagcc gtgttggcga aaccagaatg taaaattatg ctcctgtgta gcccacagaa
    30301 tcctaccggg aaagtgtgga cgtgcgatga gctggagatc atggctgacc tatgcgagcg
    30361 tcatggtgtg cgggttattt ccgatgaaat ccatatggat atggtctggg gcgagcagcc
    30421 gcatattccc tggagtaatg tagcgcgggg agactgggcg ttgctgacgt cgggctcgaa
    30481 aagtttcaat attcccgcgc tgaccggtgc ttacgggatt atagaaaata gcagcagccg
    30541 tgatgcctat ttatcggcac tgaaaggacg tgatgggctt tcttcccctt cggtactggc
    30601 gttaactgcc catatcgccg cctatcagca aggtgcaccg tggctggatg ccttacgcgt
    30661 ctatctgaaa gataacctga cgtatatctc agataaaatg aacgccgcgt ttcctgaact
    30721 caactggcag atcccgcaat ccacttatct ggcctggctt gatctacgtc tgttgaatat
    30781 tgacgacaac gcgttgcaaa aagcgcttat cgagcaagaa aaagtcgcga tcatgccggg
    30841 gtatacctat ggtgaagaag gtcgtggttt tgtccgtctc aatgccggct gcccacgttc
    30901 gaaactggaa aaaggtgtgg ctggattaat aaacgccatc cgcgctgttc gctaacccca
    30961 attgcgcaac gtaaaaaatc gttgcgcaat cgtggatttt taccctgctt tgtttttata
    31021 atggtgcgca cttttatatt cagaaaaaga gtgcgaccat gattgatacc accctgccat
    31081 taactgatat ccatcgccac cttgatggca acattcgtcc ccagaccatt cttgaacttg
    31141 gccgccagta taatatctcg cttcctgcac aatccctgga aacactgatt ccccacgttc
    31201 aggtcattgc caacgaaccc gatctggtga gctttctgac caaacttgac tggggcgtta
    31261 aagttctcgc ctctcttgat gcctgtcgcc gcgtggcatt tgaaaacatt gaagatgcag
    31321 cccgtcacgg cctgcactat gtcgagctgc gtttttcacc aggctacatg gcaatggcac
    31381 atcagctgcc tgtagcgggt gttgtcgaag cggtgatcga tggcgtacgt gaaggttgcc
    31441 gcacctttgg tgtgcaggcg aagcttatcg gcattatgag ccggaccttc ggcgaagccg
    31501 cctgtcagca agagctggag gcctttttag cccaccgtga ccagattacc gcacttgatt
    31561 tagccggtga tgaacttggt ttcccgggaa gtctgttcct ttctcacttc aaccgcgcgc
    31621 gtgatgcggg ctggcatatt accgtccatg caggcgaagc tgccgggccg gaaagcatct
    31681 ggcaggcgat tcgtgaactg ggtgcggagc gtattggaca tggcgtaaaa gccattgaag
    31741 atcgggcgct gatggatttt ctcgccgagc aacaaattgg tattgaatcc tgtctgacct
    31801 ccaatattca gaccagcacc gtagcagagc tggctgcaca tccgctgaaa acgttccttg
    31861 agcatggcat tcgtgccagc attaacactg acgatcccgg cgtacaggga gtggatatca
    31921 ttcacgaata taccgttgcc gcgccagctg ctgggttatc ccgcgagcaa atccgccagg
    31981 cacagattaa tggtctggaa atggctttcc tcagcgctga ggaaaaacac gcactgcgag
    32041 aaaaagtcgc cgcgaagtaa caaaatggat ggtgcaaatt gcaccatcca tttttcatgc
    32101 aaggcacaaa gtcgcgcgat gtttggcgga ttcgatgccc agctcaatca actccattac
    32161 ctggattgcc tggcttgccg gaaccggatt ttcaccatcg ccatttaacg catcacgaat
    32221 agccgcatag taagccggat aattcccagg taccgtcaac agcgtttctt cgacacgttc
    32281 ctcaccttcc acgcgggtaa gtacgccatc acgcatatcg tagccccagt cttcctgcgg
    32341 cagacgctcg ccatttttca gacgttcttc ctgtggatcg aggccatatt tcacataact
    32401 gcctcgggat ccatgcacga tataccgtgc tgactcagcg gctgccagca tggtaccgtg
    32461 taaaatgact cgccgctgtg gataggacaa gatggcgtgg aaataatcgg tcgactgcgc
    32521 tccgggccgt aactgtgcca aatctaccgt catgctgacc ggtaaaccaa aaagcgtaat
    32581 ggcctgatca agaagatgtg gtgctaaatc gtaccagata ccgctgcctg ggccgccctg
    32641 ttcacgccaa cgatcgcgca cctgcggacg gaagcggtca aaatgagact caaagtaagc
    32701 aacttcaccc agcacgcctt ccgcgagtaa accttttagc gtcaagaaat cgctatccca
    32761 gcgacggtta tggaatacag acagcacacg ccccaggctt tttgccagcg catccagctc
    32821 tcgcgcttgt gacagtgtca cggtaaaggg tttatcaacg accacatgtt tacccgcctc
    32881 aagcgccgct ttggctaacg ggaaatgggt atcgttgggt gtaggaatga caatcaggtc
    32941 tatgttggga tcgttaaaca gatgcttcgg ctcagagaca accgtaaccg ttggccagtc
    33001 ggcttttact tttgtttcat cactgctgga gattaccgcc agttccagcc cgggcgtgcc
    33061 cgcaatcagg ggcgcatgga aggttttgct cgcataacca tacccaatca acccaacacg
    33121 gatgttgtcg ctcatgtcat tgcctctcat tgaagtatga tggctatttg acactatcct
    33181 ttacccacgc tcaacagttt aataacctgc cagcaataag ggatgttgtt taacttaagt
    33241 caaaaaaata gcgaattttc caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa
    33301 tcttgctgga gctattattg ctaagtaaca tttaccccct gaagttaatg gatcaatcaa
    33361 gagagatgtg ggctgtaatg aatcgtctta ttgaattaac aggttggatc gttcttgtcg
    33421 tttcagtcat tcttcttggc gtggcgagtc acattgacaa ctatcagcca cctgaacaga
    33481 gtgcttcggt acaacacaag taagctctgc acttgtggag cgacatgctg cccgtccggg
    33541 tgcatgtttt cacttgtcgg atattaaacc aggaatttat tatcttgttc gatgttgttg
    33601 gtgattgtca gggatagtaa agcgcgttac tcttttcaga gcagtttttc atccctgaat
    33661 atttgccatc agcgcattct tattattgta ataacacaag cgttcacttt tggtgaaatt
    33721 ttatcatcac taataatacc tacccctttg gtattaatta ttttatggac cttttatgac
    33781 tgttcaggac tacttattaa aatttcgcaa aatcagttca ctcaaaagtc tggaaaaact
    33841 ctacgaccat cttaattaca ccctgacgga cgatcaggaa ctgatcaata tgtatcgtgc
    33901 tgccgatcac cgtcgcgcag agctggtttc tggcgggcgt ttgtttgacc tcggccaggt
    33961 accgaagtcc gtctggcact atgtccaata aagaaagtag cgatcggtta taaaagcctt
    34021 ataataacgc ccctaaaatt agatttgcct gtttgcgtcg ctattctgga gaacctatga
    34081 tcaccacaac gccacaacgt attggaggct ggttgcttgg cccgttggcc tggcttttag
    34141 tcgctctgtt gagtacgacg ctggcgttgt tgctgtatac cgctgcgtta tcttctccgc
    34201 aaacatttca aacgctcggc ggacaagcac tgacgacaca aatcttatgg ggcgtttctt
    34261 ttattaccgc tatcgccatg tggtattaca cactgtggtt gaccatcgct ttctttaaac
    34321 gtcggcgttg tgttcctaaa cactatatta tctggctgct tatttccgta ttgctggcag
    34381 taaaagcatt cgctttttca cccgtggaag atggcatagc cgttcgccag ttgctgttta
    34441 ctttgttggc aaccgcattg atcgtcccct atttcaaacg ctcgtcgcgg gtaaaagcga
    34501 cgtttgtgaa tccgtaataa ccttacagtt aacctgttgt cgcctgctct ggattaacgg
    34561 ataataggcg gcttttttat ttcaggccga aaaatgactg actacctgtt actgtttgtc
    34621 ggaactgtac tggtcaataa ctttgtactg gtcaagtttc tcggtctctg tccgtttatg
    34681 ggggtttcca aaaaactgga aaccgcgatg ggcatggggc tggcaacaac gtttgtgatg
    34741 acgctggcgt ctatttgcgc ctggcttatc gatacgtgga ttttgatccc acttaatctg
    34801 atttacctgc gcaccctggc atttattctg gtgattgctg tggtcgtgca gttcaccgag
    34861 atggtggtgc gcaaaaccag cccggtgctt taccgtctgc tggggatttt tttgccgctt
    34921 atcaccacca actgtgcagt gctcggcgtg gcgttgctga atatcaatct cgggcacaat
    34981 ttcttgcagt cggcgctgta cggtttttcc gccgctatcg gtttctcgct ggtgatggtg
    35041 ctcttcgccg ccatccgcga acgccttgct gtggctgatg tcccggcacc ttttcgcggt
    35101 catgccattg cgttaattac cgcaggtctt atgtctctgg cctttatggg ctttagtggt
    35161 ttggtgaagt tgtaatgaat gctatctgga ttgccgttgc cgccgtgagc ctgctgggcc
    35221 tggcgtttgg cgccattctg ggttatgcct cccgccgttt tgcggtggaa gacgatccgg
    35281 tcgttgagaa aattgacgaa atcttaccgc agagccagtg tggtcagtgc ggttatcccg
    35341 gctgtcgccc ctacgcggaa gccatcagct gtaacggtga aaaaatcaac cgttgcgccc
    35401 caggtggcga agctgtgatg ctaaaaattg ccgagttgct taatgtcgag ccgcagccgc
    35461 tggatggcga agcgcaagag ataacgcctg cgcggatggt ggcggttatt gatgaaaata
    35521 actgtattgg ctgcactaaa tgtattcagg cgtgtccggt agacgccatc gttggcgcta
    35581 cccgtgccat gcatacggta atgagtgatc tctgtacggg ctgcaattta tgtgttgatc
    35641 cgtgcccgac gcactgcatc tcgttgcaac cggtcgcaga aacacctgac tcctggaaat
    35701 gggatctgaa caccattccc gtgcgtatca ttcccgtgga acaccatgct taagttattc
    35761 tctgcattca gaaaaaataa aatctgggat ttcaacggcg gcatccatcc accggagatg
    35821 aaaacccagt ccaacggtac acccctgcgc caggtaccac tggcgcagcg ttttgttatt
    35881 ccactgaaac agcatattgg cgctgaaggt gagttgtgcg ttagcgtcgg cgataaagta
    35941 ttgcgcggcc agccgcttac ccgtggtcgc ggcaaaatgc tgcctgttca cgcgcccacc
    36001 tcgggtaccg ttacggctat tgcgccccac tctacggctc atccttcagc tttagctgaa
    36061 ttaagcgtga ttattgatgc cgatggtgaa gactgctgga tcccgcgcga cggctgggcc
    36121 gattatcgca ctcgcagtcg cgaagagtta atcgagcgca tacatcagtt tggtgttgcc
    36181 gggctgggcg gtgcaggatt cccgacaggc gttaaattgc agggtggcgg agataagatt
    36241 gaaacgttga ttatcaacgc ggctgagtgc gagccgtaca ttaccgccga tgaccgtttg
    36301 atgcaggatt gcgcggctca ggtcgtagag ggtattcgca ttcttgcgca tattctgcag
    36361 ccacgcgaaa ttcttatcgg cattgaagat aacaaaccgc aggcgatttc catgctgcgc
    36421 gcggtgctgg cggactctaa cgatatttct ctgcgggtga ttccaaccaa atatccttct
    36481 ggcggtgcta aacaattaac ctacattctg accgggaagc aggttccaca tggcgggcgt
    36541 tcatccgata tcggcgtatt aatgcaaaac gtcggcactg cttatgcagt gaaacgtgcc
    36601 gttattgatg gcgagccgat taccgagcgt gttgtaaccc tgactggcga agcaatcgct
    36661 cgcccgggca acgtctgggc acggctgggg acgccagtgc gtcatttatt gaatgatgcc
    36721 ggattctgcc cctctgccga tcaaatggtg attatgggtg gcccgctaat gggctttacc
    36781 ttgccatggc tggatgtccc ggtcgtaaag attaccaact gtctgttggc tccctctgcc
    36841 aatgaacttg gcgaaccaca ggaagaacaa agctgcatcc ggtgtagcgc ctgtgctgac
    36901 gcctgccctg cggatctttt gccgcaacag ttgtactggt tcagcaaagg tcagcaacac
    36961 gataaagcta ccacgcataa cattgctgat tgcattgaat gtggggcttg cgcgtgggtt
    37021 tgcccgagca atattcccct ggtgcaatat ttccgtcagg aaaaagctga aattgcggct
    37081 attcgtcagg aagaaaagcg cgccgcagaa gccaaagcgc gtttcgaagc gcgccaggct
    37141 cgtctggagc gcgaaaaagc ggctcgcctt gaacgacata agagcgcagc cgttcaacct
    37201 gcagccaaag ataaagatgc gattgctgcc gctctggcgc gggtgaaaga gaaacaggcc
    37261 caggctacac agcctattgt gattaaagcg ggcgaacgcc cggataacag tgcaattatt
    37321 gcagcacggg aagcccgtaa agcgcaagcc agagcgaaac aggcagaact gcagcaaact
    37381 aacgacgcag caaccgttgc tgatccacgt aaaactgccg ttgaagcagc tatcgcccgc
    37441 gccaaagcgc gcaagctgga acagcaacag gctaatgcgg aaccagaaga acaggtcgat
    37501 ccgcgcaaag ccgccatcga agccgctatt gcccgtgcca aagcgcgcaa gctggaacag
    37561 caacaggcta atgcggaacc agaagaacag gtcgatccgc gcaaagccgc cgtcgaagcc
    37621 gctattgccc gtgccaaagc gcgcaagctg gaacagcaac agtctaatgc ggaaccagaa
    37681 gaacaggttg atccgcgcaa agccgccgtc gaagccgcta ttgcccgagc caaagcgcgc
    37741 aaactggaac agcaacaggc taatgcggaa ccagaagaac aggttgatcc gcgcaaagcc
    37801 gccgtcgaag ccgctattgc ccgtgccaaa gcgcgcaagc tggaacagca acagactaac
    37861 gcagaaccag aagaacaggt tgatccgcgc aaagcggcag ttgccgcggc tattgcccgc
    37921 gctcaggcca aaaaagccgc ccagcagaag gttgtaaacg aggactaaat ggtattcaga
    37981 atagctagct ccccttatac ccataaccag cgccagacat cgcgcattat gctgttggtg
    38041 ttgctcgcag ccgtgccagg aatcgcagcg caactgtggt tttttggttg gggtactctc
    38101 gttcagatcc tgttggcatc ggttagtgct ctgttagccg aagctctcgt actcaaacta
    38161 cgcaagcagt cggtagccgc aacgttgaaa gataactcag cattgctgac aggcttattg
    38221 ctggcggtaa gtattccccc cctcgcgcca tggtggatgg tcgtgctggg tacggtgttt
    38281 gcggtgatca tcgctaaaca gttgtatggc ggtctggggc aaaacccgtt taatccggca
    38341 atgattggtt atgtggtctt actgatctcc ttcccggtgc agatgaccag ctggttaccg
    38401 ccacatgaaa ttgcggtcaa catccctggt tttatcgacg ccatacaggt tattttcagc
    38461 ggacataccg ccagtggtgg tgatatgaac acactacgct taggtattga tggcattagt
    38521 caggcgacac cgctggatac atttaaaacc tctgtccgtg ccggtcattc ggttgaacag
    38581 attatgcaat atccgatcta cagcggtatt ctggcgggcg ctggttggca atgggtaaat
    38641 ctcgcctggc tggctggcgg cctgtggttg ctatggcaga aagcgattcg ctggcatatt
    38701 cccctcagct tcttagtaac gctggcgtta tgcgcaacgt tgggctggtt gttctcacca
    38761 gaaacactgg cagcaccgca aattcatctg ctgtctggtg cgaccatgct cggcgcattc
    38821 tttattttga ctgacccggt taccgcttct acgaccaatc gtggtcgtct tattttcggc
    38881 gcgctggcgg gcttattagt ctggttgatc cgcagtttcg gcggctatcc tgacggcgtg
    38941 gcttttgccg tcctgctggc gaacatcacg gttcctctga tcgattacta cacgcgtccg
    39001 cgcgtctacg gccatcgcaa agggtaaacc atgctgaaaa ctatccgaaa acacggcatt
    39061 acgttagcgc tatttgcagc gggttcaaca gggttaactg cggccatcaa ccagatgacc
    39121 aaaacgacga ttgctgaaca ggccagtctg caacaaaagg cattatttga tcaggtgctg
    39181 ccagccgaac gctataacaa tgcgctggca cagagttgct atctggtaac tgcgccagag
    39241 ttaggtaaag gtgagcatcg ggtttacatc gccaaacagg atgacaaacc ggtagccgcc
    39301 gttctggaag caaccgcgcc agatggctat tccggtgcga ttcagctgct ggtgggagcc
    39361 gattttaacg gcacggtact tggcacgcgc gtgacagagc accacgaaac gccagggctt
    39421 ggcgataaaa tcgaactgcg cctttctgac tggatcaccc attttgcggg taaaaaaatc
    39481 agtggtgcag atgatgcgca ctgggcggtg aagaaagatg gtggtgattt cgaccagttc
    39541 accggcgcga cgattactcc ccgcgcggtg gttaatgcgg taaaacgcgc cggattgtac
    39601 gctcagacgt taccggcaca actttctcaa cttcctgcct gtggagaata aagcgtgagc
    39661 gaaattaaag acgttattgt tcaggggttg tggaaaaaca actctgcgct ggtccagttg
    39721 ctcggccttt gtcctctgtt ggcggtcacg tccactgcca ctaacgctct gggtttagga
    39781 cttgcgacta cgctggtact gacgctgacc aacctgacca tttcgacgct gcgtcactgg
    39841 acgccagccg agatccgcat tcccatttac gtgatgatca tcgcctcggt ggtcagcgct
    39901 gtacagatgc tgatcaacgc ctacgccttt ggcctgtatc aatcattagg gatttttatt
    39961 ccgctgattg tcactaactg tatcgttgtg ggccgcgctg aagccttcgc cgccaaaaaa
    40021 ggtccggcgc tttcggcact ggacggcttt tcaattggta tgggcgcaac ctgcgccatg
    40081 ttcgtgctgg gttcactacg cgaaattatc ggcaatggca cattgtttga cggtgcagat
    40141 gcgctgttag gtagctgggc aaaagtatta cgcgtggaaa ttttccacac cgactcccct
    40201 ttcctgctgg cgatgctgcc tccaggtgca tttattggcc tgggactgat gctggcagga
    40261 aaatacctga ttgatgaaag aatgaaaaag cgccgtactg aagcagctgc cgaacgtgca
    40321 ttgccaaacg gtgaaacagg gaatgtctga tgaacaaagc aaaacgcctg gagatcctca
    40381 ctcgcctgcg tgagaacaat cctcatccca ccaccgagct taatttcagt tcgccttttg
    40441 aattgctgat tgccgtactg ctttccgctc aggcgaccga tgtcagtgtt aataaggcga
    40501 cggcgaaact ctacccggtg gcgaatacgc ctgcagcgat gcttgaactg ggcgttgaag
    40561 gggttaaaac ctatatcaaa acgattgggc tttataacag caaagcagaa aatatcatca
    40621 aaacctgccg tatcttactg gagcagcata atggcttggt tccggaagat cgtgctgcgc
    40681 ttgaagccct gcccggcgta ggtcgtaaaa cagccaacgt cgtattaaac actgcattcg
    40741 gctggccgac tattgctgtc gacacgcaca ttttccgcgt ttgtaatcgt actcaatttg
    40801 cgccggggaa aaatgtcgaa caggtagaag aaaagctact aaaagtggtt ccggcagagt
    40861 ttaaagtcga ctgccaccat tggttgatcc tgcacgggcg ttatacctgc attgcccgca
    40921 agccccgctg tggctcttgt attattgaag atctttgtga atacaaagag aaagttgaca
    40981 tctgaagaaa aggggtaaca ccgattaccc cattgataac ctttctttat cctcttttaa
    41041 aacatctttt taacgtgaat gatgccattg cttagcgtta tcatcaggta atccgttt
//
LOCUS       DAZGTR010000027        40488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGTR010000027 DAZGTR010000000
VERSION     DAZGTR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.027"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..344
                     /locus_tag="ACHLCY_001632"
     CDS             <1..344
                     /locus_tag="ACHLCY_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0368921.1"
                     /translation="CKKQGEYFPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFY
                     SDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYLGTPNNTYLKYNPKAKG
                     NGVTIIRQGSN"
     gene            346..667
                     /locus_tag="ACHLCY_001633"
                     /pseudo
     CDS             346..667
                     /locus_tag="ACHLCY_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(754..1429)
                     /locus_tag="ACHLCY_001634"
                     /pseudo
     CDS             complement(754..1429)
                     /locus_tag="ACHLCY_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1574..2182)
                     /gene="yiiX"
                     /locus_tag="ACHLCY_001635"
     CDS             complement(1574..2182)
                     /gene="yiiX"
                     /locus_tag="ACHLCY_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP0368922.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(2367..2684)
                     /gene="metJ"
                     /locus_tag="ACHLCY_001636"
     CDS             complement(2367..2684)
                     /gene="metJ"
                     /locus_tag="ACHLCY_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP0368923.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2961..4121
                     /gene="metB"
                     /locus_tag="ACHLCY_001637"
     CDS             2961..4121
                     /gene="metB"
                     /locus_tag="ACHLCY_001637"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP0368924.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HQAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4124..6556
                     /gene="metL"
                     /locus_tag="ACHLCY_001638"
     CDS             4124..6556
                     /gene="metL"
                     /locus_tag="ACHLCY_001638"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP0368925.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPVSQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6695..6829)
                     /locus_tag="ACHLCY_001639"
     CDS             complement(6695..6829)
                     /locus_tag="ACHLCY_001639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0368926.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6905..7795
                     /gene="metF"
                     /locus_tag="ACHLCY_001640"
     CDS             6905..7795
                     /gene="metF"
                     /locus_tag="ACHLCY_001640"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP0368927.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            8124..10304
                     /gene="katG"
                     /locus_tag="ACHLCY_001641"
     CDS             8124..10304
                     /gene="katG"
                     /locus_tag="ACHLCY_001641"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP0368928.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            10398..11303
                     /gene="yijE"
                     /locus_tag="ACHLCY_001642"
     CDS             10398..11303
                     /gene="yijE"
                     /locus_tag="ACHLCY_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP0368929.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(11330..11947)
                     /gene="yijF"
                     /locus_tag="ACHLCY_001643"
     CDS             complement(11330..11947)
                     /gene="yijF"
                     /locus_tag="ACHLCY_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP0368930.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(12222..13325)
                     /gene="gldA"
                     /locus_tag="ACHLCY_001644"
     CDS             complement(12222..13325)
                     /gene="gldA"
                     /locus_tag="ACHLCY_001644"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP0368931.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(13336..13998)
                     /gene="fsa"
                     /locus_tag="ACHLCY_001645"
     CDS             complement(13336..13998)
                     /gene="fsa"
                     /locus_tag="ACHLCY_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0368932.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(14010..16511)
                     /gene="ptsP"
                     /locus_tag="ACHLCY_001646"
     CDS             complement(14010..16511)
                     /gene="ptsP"
                     /locus_tag="ACHLCY_001646"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0368933.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCRLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPMLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQAHTADGIRIEIAANIAHSVE
                     AQAAFGNSAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16820..17899
                     /gene="frwC"
                     /locus_tag="ACHLCY_001647"
     CDS             16820..17899
                     /gene="frwC"
                     /locus_tag="ACHLCY_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP0368934.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     TAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17914..18234
                     /gene="frwB"
                     /locus_tag="ACHLCY_001648"
     CDS             17914..18234
                     /gene="frwB"
                     /locus_tag="ACHLCY_001648"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0368935.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            18285..20582
                     /gene="pflD"
                     /locus_tag="ACHLCY_001649"
     CDS             18285..20582
                     /gene="pflD"
                     /locus_tag="ACHLCY_001649"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0368936.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            20548..21426
                     /gene="pflC"
                     /locus_tag="ACHLCY_001650"
     CDS             20548..21426
                     /gene="pflC"
                     /locus_tag="ACHLCY_001650"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP0368937.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            21428..21769
                     /gene="frwD"
                     /locus_tag="ACHLCY_001651"
     CDS             21428..21769
                     /gene="frwD"
                     /locus_tag="ACHLCY_001651"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0368938.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21756..22607)
                     /gene="yijO"
                     /locus_tag="ACHLCY_001652"
     CDS             complement(21756..22607)
                     /gene="yijO"
                     /locus_tag="ACHLCY_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0368939.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22756..24489)
                     /gene="eptC"
                     /locus_tag="ACHLCY_001653"
     CDS             complement(22756..24489)
                     /gene="eptC"
                     /locus_tag="ACHLCY_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0368940.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLHPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24672..27323)
                     /gene="ppc"
                     /locus_tag="ACHLCY_001654"
     CDS             complement(24672..27323)
                     /gene="ppc"
                     /locus_tag="ACHLCY_001654"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP0368941.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27675..28826)
                     /gene="argE"
                     /locus_tag="ACHLCY_001655"
     CDS             complement(27675..28826)
                     /gene="argE"
                     /locus_tag="ACHLCY_001655"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP0368942.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28980..29984
                     /gene="argC"
                     /locus_tag="ACHLCY_001656"
     CDS             28980..29984
                     /gene="argC"
                     /locus_tag="ACHLCY_001656"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP0368943.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPCCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29992..30768
                     /gene="argB"
                     /locus_tag="ACHLCY_001657"
     CDS             29992..30768
                     /gene="argB"
                     /locus_tag="ACHLCY_001657"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP0368944.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINTLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30829..32202
                     /gene="argH"
                     /locus_tag="ACHLCY_001658"
     CDS             30829..32202
                     /gene="argH"
                     /locus_tag="ACHLCY_001658"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP0368945.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVGELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            32469..33386
                     /gene="oxyR"
                     /locus_tag="ACHLCY_001659"
     CDS             32469..33386
                     /gene="oxyR"
                     /locus_tag="ACHLCY_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP0368946.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(33369..34769)
                     /gene="sthA"
                     /locus_tag="ACHLCY_001660"
     CDS             complement(33369..34769)
                     /gene="sthA"
                     /locus_tag="ACHLCY_001660"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP0368947.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            35100..35750
                     /gene="fabR"
                     /locus_tag="ACHLCY_001661"
     CDS             35100..35750
                     /gene="fabR"
                     /locus_tag="ACHLCY_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP0368948.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35750..36109
                     /gene="yijD"
                     /locus_tag="ACHLCY_001662"
     CDS             35750..36109
                     /gene="yijD"
                     /locus_tag="ACHLCY_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP0368949.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYSLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(36149..37249)
                     /gene="trmA"
                     /locus_tag="ACHLCY_001663"
     CDS             complement(36149..37249)
                     /gene="trmA"
                     /locus_tag="ACHLCY_001663"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP0368950.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      37363..37553
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37618..39462
                     /gene="btuB"
                     /locus_tag="ACHLCY_001664"
     CDS             37618..39462
                     /gene="btuB"
                     /locus_tag="ACHLCY_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP0368951.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGASL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            39407..40264
                     /gene="murI"
                     /locus_tag="ACHLCY_001665"
     CDS             39407..40264
                     /gene="murI"
                     /locus_tag="ACHLCY_001665"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP0368952.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9319 a        10098 c        11146 g         9925 t
ORIGIN      
        1 tatgtaagaa gcaaggtgaa tatttcccca tgaatccgat aagttcaaat gataatgata
       61 gtgtagactt gcgaaatata aaatgccctg aaggttcaga gagagtaggc gattatcaca
      121 ctcacggttt ttactctgac gataaaggaa ataaagtaac aaaagaaaat gatgtttatg
      181 atagtctaaa tttttcaagc aaagatttaa cgaattctta tatgaatgga atgggaaaaa
      241 aagaatacag tagttacttg ggaacaccaa ataacaccta tctaaaatat aatcccaaag
      301 ctaaagggaa tggagttaca attatcaggc aagggagtaa ttaaaatgaa ggcaatacaa
      361 tgttgttatt tccttgcttt agtctccagt catttaatgg ctggagttat gggtgatgat
      421 aatatatttt taagtaattc ggattcagct gctttatatt gtgtggctaa aatatttcca
      481 gatggagaaa atattttaat atataaagta aatataatta atgctagttc aaaaaaagat
      541 gtttttttat gaaagcccaa catcaaccca caatgacata atggggggcg ggggaatgtc
      601 atatgcctat gaatatgatg taaaatataa tatatgtcat ttgagaaaaa agagcagaat
      661 gaggtaaaaa tctttggctg tgtattcagt ctggtaatca tccaaggaaa gcttatcaga
      721 agggtgatga ctccaattag tagaacatat attttcgata aaagcaccga tgactttttc
      781 atgaagttct acagaacgcg agaaacagat ggttctgcgg gccagacgtt tgattcgggt
      841 tctcagggtc agattgttac gctcaatgtg ctgagtaaaa atcttaccag tcagatgctt
      901 ctccttcggt aactctctag cgtaactacc ccattcgtca gtggtcaaca tgccgatggt
      961 aaaagggtaa gcagcgcccg caattcacga caggtttcgt cagtccgggg gccaaaagtg
     1021 taggccagaa caccaccagt tttcgtgttg tacacgtacc agagccagtg ctgtcgcgct
     1081 ttactgccga caaagctcca ttgctgacta agtttgcaga tgagtgccac atcagcatga
     1141 gcgaccgggg atgacgttat tcttcgtggc gagagttttt taaagttcgg atgactgtat
     1201 taatgccgat tttcagcgta cgggcagtat cgcgaacccc ggcaccgttg aaagtcattt
     1261 cagcgatttg gtctttaact cccggtttac ggggctcgta acagtaactg agctgaaata
     1321 cccggtgaca gtcacggccg cggaatcgat catgtctttt tgggttctgc ccgtgacggt
     1381 aaacctgaac ggactggcaa cgggggcaat gaaggttaac tgtgaccatg aaacaacctt
     1441 aaaagcgagt attatatcct taattcaact aattggaggc atcaccgttt accggagcga
     1501 cagcttgcgg aaaggtcgtg caactacaaa gcaacaaaca gactacggcg ggaattattt
     1561 ttttcatcat ccattaccac gaaaacagcg gccactcttt ggcaaccgtg gtgagttgtg
     1621 gcgcgtcgaa aacggcttgt ggtgaaacta ccgtttcctc aagcggaata tttttaccgt
     1681 agcgttcttt gagcttcgcc tggaccagcg ggttgctgag atcaaactct ttcaacttct
     1741 gctgttcgcc cacgcgcatt cccagcgcgt tctggtaaac cttccacacc acttccgaac
     1801 agtactggcg atcgtcgctc catgagaagc tgaaatcata cggtttaccg agataacgtt
     1861 ttgccgtttg cgccagtttt tgctgttgtt caacactcag tccgccttca acgcggcgaa
     1921 cgacgtattt gcccttttca ccatgggcaa tccactgctt gagcggggtg tatttcaccg
     1981 ggccgaccgc ttcaaaaatg tagggctttt tgttacgcat caccagcata ccggtgtggc
     2041 tgtaatcgga atgggttgcc agttggatcg ctttactttg cgatgagcga gagatttgaa
     2101 agatgatgtc gccagtttgc ggctgccagg caaaggcagg tacagaaacc agcaggctga
     2161 ggatcagcag cctgtttttc atagttaaac gtccatgtat aaaaagcggt gggtcgcaga
     2221 caacgtgatc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc tacaagttcg
     2281 tgcaaattca ataaattgca atatgacgta gatactgata agcgtagcgc atcaggcgat
     2341 tccactccgc gccgctcatt tccgctttag tattcccacg tctccgggtt aatccccatc
     2401 tcacgcatga tctcttttgc cgcttccggg atttcgtcgc tgcgctcttt acgcagatcg
     2461 gcatcatccg gcaaaggttg cccggtaaag gcatgcagaa acgcttcgca cagcagctcg
     2521 ctgttggtag cgtgacgcag gttgttcacc tgacgacgcg tgcgttcatc ggtgaggatt
     2581 tttaacacct taagaggaat ggaaaccgta atctttttga cttgttcact cttcttgccg
     2641 tgctcagcgt atgggctgat atattcgccg ctccattcag ccatgagata cttaatcctc
     2701 ttcgtcaata aattgagacc agaccacaca atttatgtgg gtactgaccg taaacccgca
     2761 tagtttaccg tacaggcgtt accgtgacat cgtgtaatgc acctgtcggc gtgataatgc
     2821 atataatttt aacggctatt tgggatttgc tcaatctata cgcaaagaag tttagatgtc
     2881 cagatgtatt gacgtccatt aacacaatgt ttactctggt gcctgacatt tcaccgacaa
     2941 agcccaggga acttcatcac atgacgcgta aacaggccac catcgcagtg cgtagcgggt
     3001 taaatgacga cgaacagtat ggttgcgttg tcccaccgat ccatctttcc agcacatata
     3061 actttaccgg atttaatgaa ccgcgcgcgc atgattactc gcgtcgcggc aacccaacgc
     3121 gcgatgtggt tcagcgtgcg ctggcagaac tggaaggcgg tgctggtgca gtacttacta
     3181 ataccggcat gtccgcgatt cacctggtaa tgaccgtctt tttgaaacct ggcgatctgc
     3241 tggttgcgcc gcacgactgc tacggcggta gctatcgcct gttcgacagc ctggcgaaac
     3301 gcggttgcta tcgcgtgttg tttgttgatc aaggcgatga acaggcatta cgggcagcac
     3361 tggcagaaaa acccaaactg gtactggtag aaagcccaag taatccattg ttacgcgtcg
     3421 tggatattgc gaaaatctgc catcaggcaa gggaagtcgg ggcggtgagc gtggtggata
     3481 acaccttctt aagcccggca ttacaaaatc cgctggcatt gggtgccgat ctggtgttgc
     3541 attcatgtac gaaatatctg aacggtcact ctgacgtggt ggctggcgtg gtgattgcta
     3601 aagacccgga cgttgtcact gaacttgcct ggtgggcaaa caatattggc gtgacgggcg
     3661 gcgcgtttga cagctatctg ctgctacgcg ggttgcgaac gctggtgccg cgtatggagc
     3721 tggcgcagcg caacgcgcag gcgattgtga aatatctgca aacccagccg ttggtgaaaa
     3781 aactgtatca cccgtcgctg ccggaaaatc aggggcatga aattgccgcg cgtcagcaaa
     3841 aaggctttgg cgcaatgttg agttttgaac tggatggcga tgagcagacg ctgcgtcgtt
     3901 tcctgggcgg gctgtcgttg tttacgctgg cggaatcatt agggggagtg gaaagtttaa
     3961 tctctcacgc cgcaaccatg acacatgcag gcatggcacc agaagcgcgt gctgccgccg
     4021 ggatttccga gacgctgctg cgtatctcca ccggtattga agatggcgaa gatttaattg
     4081 ccgacctgga aaatggcttc cgggctgcaa acaaggggta aaaatgagtg tgattgcgca
     4141 ggcaggggcg aaaggtcgtc agctgcataa atttggtggc agtagtctgg ctgatgtgaa
     4201 gtgttatttg cgtgtcgcgg gcattatggc ggagtactct cagcctgacg atatgatggt
     4261 ggtttccgcc gccggtagca ccactaacca gttgattaac tggttgaaac taagccagac
     4321 cgatcgtctc tctgcgcatc aggttcaaca aacgctgcgt cgctatcagt gcgatctgat
     4381 tagcggtctg ctacccgctg aagaagccga tagcctcatt agcgcttttg tcagcgacct
     4441 tgagcgcctg gcggcgctgc tcgacagcgg tattaacgac gcagtgtatg cggaagtggt
     4501 gggccacggg gaagtatggt cggcacgcct gatgtctgcg gtacttaatc aacaagggct
     4561 gccagcggcc tggcttgatg cccgcgagtt tttacgcgct gaacgcgccg cacaaccgca
     4621 ggttgatgaa gggctttctt acccgttgct gcaacagctg ctggtgcaac atccgggcaa
     4681 acgtctggtg gtgaccggat ttatcagccg caacaacgcc ggtgaaacgg tgctgctggg
     4741 gcgtaacggt tccgactatt ccgcgacaca aatcggtgcg ctggcgggtg tttctcgcgt
     4801 aaccatctgg agcgacgtcg ccggggtata cagtgccgac ccgcgtaaag tgaaagatgc
     4861 ctgcctgctg ccgttgctgc gtctggatga ggccagcgaa ctggcgcgcc tggcggctcc
     4921 cgttcttcac gcccgtactt tacagccggt ttctggcagc gaaatcgacc tgcaactgcg
     4981 ctgtagctac acgccggatc aaggttccac gcgcattgaa cgcgtgctgg cctccggtac
     5041 tggtgcgcgt attgtcacca gccacgatga tgtctgtttg attgagtttc aggtgcccgt
     5101 cagtcaggat ttcaaactgg cgcataaaga gatcgaccaa atcctgaaac gcgcgcaggt
     5161 acgcccgctg gcggttggcg tacataacga tcgccagttg ctgcaatttt gctacacctc
     5221 agaagtggcc gacagtgcgc tgaaaatcct cgacgaagcg ggattacctg gcgaactgcg
     5281 cctgcgtcag gggctggcgc tggtggcgat ggtcggtgca ggcgtcaccc gtaacccgct
     5341 gcattgccac cgcttctggc agcaactgaa aggccagccg gtcgaattta cctggcagtc
     5401 cgatgacggc atcagcctgg tggcagtact gcgcaccggc ccgaccgaaa gcctgattca
     5461 ggggctgcat cagtccgtct tccgcgcaga aaaacgcatc ggcctggtat tgttcggtaa
     5521 gggcaatatc ggttcccgtt ggctggaact gttcgcccgt gagcagagca cgctttcggc
     5581 acgtaccggc tttgagtttg tgctggcagg tgtggtggac agccgccgca gcctgttgag
     5641 ctatgacggg ctggacgcca gccgcgcgtt agccttcttc aacgatgaag cggttgagca
     5701 ggatgaagag tcgttgttcc tgtggatgcg cgcccatccg tatgatgatt tagtggtgct
     5761 ggacgttacc gccagccagc agcttgctga tcagtatctt gatttcgcca gccacggttt
     5821 ccacgttatc agcgccaaca aactggcggg agccagcgac agcaataaat atcgccagat
     5881 ccacgacgcc ttcgaaaaaa ccgggcgtca ctggctgtac aatgccaccg tcggtgcggg
     5941 cttgccgatc aaccacaccg tgcgcgatct gatcgacagc ggcgatacta ttttgtcgat
     6001 cagcgggatc ttctccggca cgctctcctg gctgttcctg caattcgacg gtagcgtgcc
     6061 gtttaccgag ctggtggatc aggcgtggca gcagggctta accgagcctg atccgcgtga
     6121 cgacctctcc ggcaaagacg taatgcgcaa gctggtaatt ctggcgcgtg aagcgggtta
     6181 caacattgaa ccggaccagg tacgtgtgga atcgctggtg cctgctcatt gcgaaggcgg
     6241 cagcatcgac catttctttg aaaatggcga tgaacttaac gagcagatgg tgcaacggct
     6301 ggaagcggcc cgcgaaatgg ggctggtgct gcgctacgtg gcgcgtttcg atgccaatgg
     6361 caaagcgcgt gtgggcgtgg aagcggtgcg tgaagaccat ccgctggcat cactgctgcc
     6421 atgcgataac gtctttgcca tcgaaagccg ctggtaccgc gataatccgc tggtgatccg
     6481 tgggcctggc gctgggcgcg acgtcaccgc cggggcgatt cagtcggata tcaaccggct
     6541 ggcacagttg ttgtaatttc agaaatttaa taatgcccgg tactcatgtt ttcgggtcta
     6601 tggtttctaa tgaaatatat tgaattatca taggattagg ccggatgaag cgtttacgac
     6661 aaatccggca agaagcaata agtacatggt tagtttatat ttgcagtccg gtttgctttg
     6721 cataccggat tttctttttc ttaccatcct gaagtttttt catcttccct gatttttcct
     6781 caccatcatt ggtcattttt cggttgacgc ccttcggctt ttccttcatc tttacatctg
     6841 gacgtctaaa tggatagatg tgcacaacac aacatataac tacaagcgat tgatgaggta
     6901 aggtatgagc ttttttcacg ccagccagcg ggatgccctg aatcagagcc tggcagaagt
     6961 ccaggggcag attaacgttt cgttcgagtt tttcccgccg cgtaccagtg aaatggagca
     7021 gaccctgtgg aactccatcg atcgccttag cagcctgaaa ccgaagtttg tatcggtgac
     7081 ctatggcgcg aactccggcg agcgcgaccg tacgcacagc attattaaag gcattaaaga
     7141 tcgcactggt ctggaagcgg caccgcatct tacttgcatt gatgcgacgc ccgacgagct
     7201 gcgcaccatt gcacgcgact actggaataa cggtattcgt catatcgtgg cgctgcgtgg
     7261 cgatctgccg ccgggaagtg gtaagccgga aatgtatgct tctgacctgg tgacgctgtt
     7321 aaaagaagtg gcagatttcg atatctccgt ggcggcgtat ccggaagttc acccggaagc
     7381 aaaaagcgct caggcggatt tgctcaacct gaaacgcaaa gtcgatgccg gagccaaccg
     7441 cgcgattact cagttcttct ttgatgtcga aagctacctg cgttttcgtg accgctgcgt
     7501 atcggcgggc attgatgtgg aaattattcc ggggattttg ccggtatcta actttaaaca
     7561 ggcgaagaaa tttgccgata tgaccaacgt gcgtattccg gcgtggatgg cacaaatgtt
     7621 tgacgggctg gatgacgatg ctgaaacgcg caagctggtg ggcgcgaata ttgccatgga
     7681 tatggtgaag attttaagcc gtgaaggagt gaaagatttc cacttctata cgcttaaccg
     7741 cgctgaaatg agttacgcga tttgccatac gctgggtgtt cgacctggtt tataaatagt
     7801 gtggcttttg tgaaaatcac actgtgatca caaattttaa acagagcaca aaatgctgcc
     7861 tcgaaatgag ggcgtaaaaa taaggttgtc agctttgttt tcgccctcct tccttgaaga
     7921 ataggaatct aaaacccttt tttataaagc agttgtccga attcggacat aatcaaaaaa
     7981 gcttaattac gatcaatttg atctacatct ctttaaccaa caatatgtaa gatctcaact
     8041 atcgcatccg tggattaatt caattataac ttctctctaa cgctgtgtat cgtaacggta
     8101 acactgtaga ggggagcaca ttgatgagca cgtcagacga tattcataac acgacagcca
     8161 ctggcaaatg cccgttccat cagggcggtc acgaccagag cgcgggggcg ggcacaacca
     8221 ctcgcgactg gtggccaaat caactccgtg ttgacctgtt aaaccaacat tctaatcgtt
     8281 ctaacccgct gggtgaggac tttgactacc gcaaagaatt cagcaaatta gattactacg
     8341 gcctgaaaaa agatctgaaa gccctgctga cagaatctca gccgtggtgg cctgccgact
     8401 ggggcagcta cgccggtctg tttattcgta tggcctggca cggcgcgggg acttaccgtt
     8461 caatcgatgg acgcggcggc gcgggtcgtg gtcagcaacg ttttgcaccg ctgaactcct
     8521 ggccggataa cgtaagcctc gataaagcgc gtcgcctgtt gtggccaatc aaacagaaat
     8581 atggtcagaa aatctcctgg gccgacctgt ttatcctcgc gggtaacgtg gcgctggaaa
     8641 actccggctt ccgtaccttc ggttttggtg ccggtcgtga agacgtctgg gaaccggatc
     8701 tggatgttaa ctggggtgat gaaaaagcct ggctgaccca ccgtcatccg gaagcgctgg
     8761 cgaaagcacc gctgggtgca accgagatgg gcctgattta cgttaacccg gaaggcccgg
     8821 atcacagcgg cgaaccgctt tctgcggcag cagctatccg tgcgaccttc ggcaacatgg
     8881 gcatgaacga cgaagaaacc gtggcgctta ttgcgggtgg tcatacgctg ggtaaaaccc
     8941 acggtgccgg tccgacatca aatgtaggtc ctgatccaga agctgcaccg attgaagaac
     9001 aaggtttagg ttgggcgagc acttacggca gcggcgttgg cgcagatgcc attacctctg
     9061 gtctggaagt agtctggacc cagacgccga cccagtggag caactatttc ttcgagaacc
     9121 tgttcaagta tgagtgggta cagacccgca gcccggctgg cgcaatccag ttcgaagcgg
     9181 tagacgcacc ggaaattatc ccggatccgt ttgatccgtc gaagaaacgt aaaccgacga
     9241 tgctggtgac cgacctgacg ctgcgttttg atcctgagtt cgagaagatc tctcgtcgtt
     9301 tcctcaacga tccgcaggcg ttcaacgaag cctttgcccg tgcctggttc aaactgacgc
     9361 acagggatat ggggccgaaa tctcgctaca tcgggccgga agtgccgaaa gaagatctga
     9421 tctggcaaga tccgctgccg cagccgatct acaacccgac cgagcaggac attatcgatc
     9481 tgaaattcgc gattgcggat tctggtctgt ctgttagtga gctggtatcg gtggcctggg
     9541 cttctgcttc taccttccgt ggtggcgaca aacgcggtgg tgccaacggt gcgcgtctgg
     9601 cattaatgcc gcagcgcgac tgggatgtga acgccgcagc cgttcgtgct ctgcctgttt
     9661 tggagaaaat ccagaaagag tctggtaaag cctcgctggc ggatatcatc gtgctggctg
     9721 gtgtggttgg tgttgagaaa gccgcaagcg ccgcaggttt gagcattcat gtaccgtttg
     9781 cgccgggccg cgttgatgcg cgtcaggatc agactgacat tgagatgttc gagttgctgg
     9841 agccaattgc tgacggtttc cgtaactatc gcgctcgtct ggatgtttcc accaccgagt
     9901 cactgctgat cgacaaagcc cagcaactga ccctgaccgc gccggaaatg accgcgctgg
     9961 tgggcgggat gcgtgtactg ggtgccaact tcgatggcag caaaaacggc gtcttcactg
    10021 accgcgttgg cgtattgagc aatgacttct tcgtgaactt gctggatatg cgttacgagt
    10081 ggaaagcgac cgacgaatcg aaagagctgt ttgaaggccg tgaccgcgaa accggcgaag
    10141 tgaaatacac tgccagccgt gcggatctgg tatttggttc taactctgtc ctgcgtgcgg
    10201 tggctgaagt ttacgccagc agcgatgctc acgagaagtt tgttaaagac ttcgtggcgg
    10261 catgggtgaa agtgatgaac ctcgaccgtt tcgacctgct gtaatctgac cttcttcagc
    10321 gactgccttt caggcagtcg ctgaagtttc tttaccggcg tatagtgtcc acaggaaaac
    10381 tacacactgg atctctcatg tctgtcgcag gaaagagcaa cccactggca atcagtggcc
    10441 tggttgtgct cacacttatc tggagttata gctggatttt catgaagcaa gtcaccagct
    10501 acatcggtgc cttcgacttt accgccttac gctgcatttt cggcgctctc gttttattca
    10561 tcgtcctttt attacgtggt cgcggaatgc gcccgacacc gtttaaatac accttagcca
    10621 ttgccctgtt acaaacctgc gggatggttg gcctggcgca gtgggcgctg gtcagcggcg
    10681 gagcggggaa ggtggcgatc ctaagctata ccatgccgtt ctgggtggtg attttcgccg
    10741 cgttgtttct cggtgaacgc ctgcgacgtg ggcaatattt cgcgatactg attgccgctt
    10801 tcggcttatt tttggtgttg cagccgtggc aactcgattt ctcttcgatg aaaagtgcca
    10861 tgctggcaat cctctccggc gtcagttggg gggcgagcgc gattgttgct aaacgtctgt
    10921 atgcccgtca tccgcgcgtg gatttattgt cgttaacatc ctggcagatg ctctatgcgg
    10981 cgctggtgat gagtgtggtc gctttactgg tgccgcaacg tgaaattgac tggcagccca
    11041 ccgtgttctg ggcgctggcc tacagtgcga ttctggcgac ggcgctggcg tggagcttat
    11101 ggttgtttgt attgaaaaac ttgcctgcca gtattgccag cttaagcacg ctggccgttc
    11161 ccgtttgcgg cgtactcttt tcctggtggc tgctcggtga gaatccgggg gccgttgaag
    11221 gtagcggtat tgtgctgatt gtgctggcac tggcgctggt gagccgtaag aaaaaagaag
    11281 ctgtcagtgt aaaaagcatc tgaatttttt cttcatgtgg ggcgatcgct tatttaacaa
    11341 aataacgata atgccccacc atccgccagc taaacagcac atcttcttcc tgcgcgcctg
    11401 cgccaatgtt atgtatcacc agcggcgtac cgtcgcgggc gaagccatct gacaccaccc
    11461 caatatgtgc cagtccgtta tccagtcgcc aggagacaat atcgcccgct tgatagtcac
    11521 tggggttctt gctgatgggg cgtgttttat cgtggcgggt aaaccaggtt tccagattag
    11581 gcacccgacg gtgatcgatg ttgctgtccg ggcgctttaa ctgccatttt tgcgggtact
    11641 cggcaaaatt cttcgccata tcttcgtgca ccagtttctg caaatcgacc ttctggctgc
    11701 gcaatgcacg gatcaccaca tcggaacata ccccgcgttc ttgcgggaca tcaccgccag
    11761 gataggtaag ctgcatatac gccggatcgt aaaatagcgt gctgccaatt tgctgtctgg
    11821 caccgtctgc gatggcaagg ttggtattgg cctggatttg taccacggtt ggtggaacgg
    11881 cgggagattt taaggagtgg ctggtaaatg ccgttagcag gctgagcagc gccagtgaag
    11941 ctttcatctg gttcgtccgt gaaaataaga gtcacagatt cgaccttccg ggctaacgat
    12001 tgttctgtag agaaaatgtc cagaagcgga taaaaaactc ctgaacaacg taggagtttc
    12061 agattactgg cacagagtgc cggatgcggc gtgaacgcct tatctggcct aaaaagcacg
    12121 caaattcaat atattgcaga gattgcgtag atcctgataa gcgtagcgca tcaggcaatt
    12181 ttgcgttcaa actcccggac aagccgggag tttggagtag gttattccca ctcttgcagg
    12241 aaacgctgac cgtactggtc ggctaccagc agagcggcgt aaacctgatc tggcgtcgcg
    12301 ccgccaggca tgttgtggat ggtttcacct tctgcacatg ccgcttctgc cacaattcgc
    12361 attttcgccg ggacatcttc tttaatatcc agttgagcga gagttattgg caaacctacc
    12421 gcatggctaa gtgcagctac ggtttcgatt tcctcaaccg gcgcgttttc cagtaccagc
    12481 tgcgtcagcg taccgaatgc cactttttca ccgtgataat agtgatgcgc gtccgggata
    12541 gcggtcaggc cgttatgcac cgcatgagcc gccgccagtc cgccgctttc aaaaccaacg
    12601 ccgcttaaat aggtgttcgc ttcaatcacg cgttccagcg caggggttac tacatgctgt
    12661 tcggcggcaa gcatcgcttt ttcgccttct tccagcagag tgttgtagca cagttctgcc
    12721 agtgccagcg ccgcctgggt gcacttgccg cccgccatag tggtagcgcc gctacgggaa
    12781 caggcgcgtg cttcaaacca ggttgccagc gcatcgccga tacccgccgc taacagacgt
    12841 gcaggagcgc ccgcgacgat tttggtgtcg acaatgacca tattcgggtt atttggcaac
    12901 agtagatagc ggtcaaactc accctcatcg gtgtagataa cagacaatgc gctgcacggt
    12961 gcatcggtag aggcgatagt cggtgcgatc gctaccggaa cacccatgaa atgtgccagt
    13021 gctttggcag tatcgagggt ttttccgcca ccgataccga gaattgcgcc acactgcgca
    13081 gtctccgcga tgccacgcag acgatcgatc tcattttgcg aacattcacc gccaaacggc
    13141 gcaatttcta ctaccagtcc agcatcttta aagcttttct cgacagtgga ttgagcaaaa
    13201 cctaaaacaa atttgtcacc caccactaac cagcgttctg ccagcggctt caggtattcg
    13261 cccagacgat taatcacatc agcgccctgg atgtatttac ccggtgattg aataatgcgg
    13321 tccataattg ctcctttaga gatgagtagt gccaaatgcg gcattccagt cgtgttcgaa
    13381 cttctctata gctgactcta ccgcaggggt gttgagcatt tgttgcgcta catctaaagg
    13441 cagggtgatg gactcacatc ctgccagcaa acaatccagt gcctgacgcg gcgttttaaa
    13501 gctggctgca agcaccatgc tttctggcgc gtgcatttct aacagcgctt gcagctcctg
    13561 aaccgtacga atgccgtccc cgccctgggc atctacgcgg ttaacataag gagcaacgta
    13621 ttttgcccct gccagcgcgg ccagtaaccc ttgtgcagca ctgtacactg cggtgccaag
    13681 cgtagtaata ccctcttttt tcagcatctt aatggcggcc agaccttcgg aagtcaccgg
    13741 gattttcacc acaatgcccg gaatggcgtc gcgcaggcgc ttcgcttctt ccaccatacc
    13801 ctgcgcgtca cgactcatag tctgagcaaa cagaatgccc tcatcaccaa tcgctttttg
    13861 cagacgcggc agaacttccc atatggactc cttgctggca gcgataatgc tcgggttagt
    13921 tgtcaccccg gcaatgggga atatgcgtgc cagacgttcg acttctgcga cgttagcggt
    13981 gtccagatac agttccatga tgtttccttt tacagttcca gttcatgttg cagcaggctg
    14041 gcgatagcgt cggcagaggc ggcgttaacc agcgcgttac ggaattcttc gtgcatgatg
    14101 cggcgagcga ggcgcgagaa aatgcgcatg tgctgatcgc ccgcagcgtg tttgttcagg
    14161 gttaacataa tgatgaattg cgcttcatca tcgccccagc gcaccggcgc ttgcagacgc
    14221 gctacactga tggtggattg ctcaatgtgt tctgatttgc tgtggggaat ggcaaaacta
    14281 aagcccagac cggtagagaa aacggcctcg cgcgcccaca agtcggcttc cagtttacgc
    14341 ggatagcggc agcggcccgc cagcagcagg ttatcggtca tgcctttgag cacttcttct
    14401 ttgctgcgcc agtcgctttc cagcgtgatg cactcggcgg tgaccagcgg tgcgtcctgt
    14461 tgggtcatgc ggaattgcgc cagcaggtgt tccacttcca gcgaagtacg gcaggccatt
    14521 gcctggttga gcaactggcg gcattcgcgg ctatcaagtt gcgccattcg cgctttcgcc
    14581 gccggaattg acggtgcgct catgctcagc tcatccagcc ctaaaccgac cagcaacggc
    14641 agcacggaac ctttcgctcc cagctcaccg cacagaccaa tccatttgcc ctggcgatgc
    14701 accgcttgca cggcgtaatc gagcgcccgc aagaatgccg gattcaggct gttgtagtga
    14761 cgagtaacct tagcattatc gcgatccacc gccagcagat attgcgtcag gtcattacta
    14821 ccaatactaa agaaatcaat ctcttcgcag cattgatcga tgatgaacat caccgacggc
    14881 acttccagca taatgccgag ctggatcttc tcatcaaacg gaatgtgttc gttacgcagt
    14941 tgctgtttgg cttccgccag tttttctttc acccataaga tctcttccat tgaagagatc
    15001 atcgggatca tgattttcag gctgccgtga gcggaggcgc gcaggatcga ccgtagttgt
    15061 gtggtgaaca gcgaggcgta ttcttcataa atacgcacag cgcgataacc gaggaacggg
    15121 ttagcttctg ccggaatgtt cagataatca acgggtttgt cgccgccaat gtccatagtg
    15181 cgcacaataa tgctgcgtcc gttggcagac tccagcgcct gacaaaaaat gttgtacagc
    15241 tcgctttcgc ccggtgcact ggtgcgatcc atatagagca tttcagtgcg gaacaaacca
    15301 acgccttccg cactattgcc gaatgcggcc tgcgcttcca cggagtgagc gatgttagcg
    15361 gcaatttcaa tgcggatacc gtcagcggta tgggcttgtt gggtcagcca gacacgctgt
    15421 tgctcacgca gggcatcctg tacgcgggct tcttgctgat aataacgggc tacggcttcc
    15481 cctggctcaa ccacaatcgc tccggcgttg ccgtcgatat aaatcgtttg atgttgccac
    15541 ggggtaaggg catcaatatc cacaccaacc agcatcggaa tgttgaacga acgggcaagg
    15601 atcaccgtat gtgaggtggt gccgccgctt ttgagcaaca atcctttgag gtgattttta
    15661 tccagttcga ggaactggct gggggtcagt tcatcagcca tacaaatggc gggctgcgtc
    15721 agtttgcccg gtgccgggaa gcgttgctca ccgtagattt gctggagtaa ctggaagcag
    15781 acgtcgcgta cgtccagggc acgttcttgc aggtagctgc tgctggaacg ggaaaactct
    15841 tcacaaaagt gattcgcact ggcgacaatc gcttcggcgc agcttaatcc ggcgctgacg
    15901 cctgccagta aatgttcgtg cagggaagta tcgccagcca gggatcggtg cgcttccaga
    15961 atcgcgctgg tggcaccgtc gctatccagc agacgaaact caatgttttt cagtaccagc
    16021 gtcaggccgt tttccagtgc ggattgctcg gcgtcaacgt ctttggctgc gggaagatta
    16081 ctcagcgcat tgagatctaa agaagagatc ggcgtcagaa tgccgcctgc gctaccgctg
    16141 cacacggtgc gggcgcggat aatttgcgga ttcagattgg tcagtgaaac cggcagtggt
    16201 tccagttcgt cagatttaac ttccgccagc ggtgcgtcgc agtgggggaa ttcatcgcgc
    16261 agccattggc ttaaacgctg gtgcgcttcc tgttcgtcgg ccccggaaat cagtaaccgg
    16321 cagttatcgc ccgccagcgt gtcggtgcca atcagcgcaa gggcgctttt ggcgttgccc
    16381 ttgcggtcag tgcgcaggtt atgccactca atttgtgatg aaaaagtatt acacagcgtt
    16441 tcaacgtggc ttgccggacg tgcatgtacg ccgttaggta gctcacaaat aaattccaca
    16501 atcagggcca ttgcctctct cccggtacgg ttttctgttt acaggataag agcgcacggc
    16561 aacggcctgc catgtgacaa atctgccaaa agctggacaa atgtaatgta accgtcaatt
    16621 tgcgacgcgt ctcacaagac gccgttttgc ggcatgcttc cggattatcg caagttatga
    16681 ggcggatcgc atttttgtac tgatattaca aaagtccagt aaatggcctt tttatccact
    16741 gtttgctccg cctgcgattg cctattgttt cctccatcaa aaatatggac atgggctttc
    16801 gcaagcccgg gagcagctta tgaatgagtt ggtgcagatc ctgaaaaata cccgtcagca
    16861 tttaatgacg ggcgtttcac acatgattcc cttcgtggta tcgggcggta ttttgctggc
    16921 ggtttccgtc atgttgtatg gcaaaggcgc agtgccggat gccgtagccg atccgaatct
    16981 gaaaaaactg tttgatatcg gagttgcagg tttgacgctg atggtgcctt tcctcgccgc
    17041 ttacatcggt tactccattg cagagcgttc tgcgctggcc ccgtgcgcta tcggtgcctg
    17101 ggttggtaac agctttgggg cgggcttctt tggtgcgctg atcgccggga ttatcggcgg
    17161 catcgtggtg cattacctga agaaaattcc ggtgcataaa gttctgcgct cggtgatgcc
    17221 tatcttcatt attcctatcg tcggcacact gattaccgca ggcatcatga tgtggggctt
    17281 gggcgagcct gtaggggcgt tgaccaacag cctgactcag tggcttcagg ggatgcagca
    17341 gggcagcatt gttatgctgg cggtgatcat gggtctgatg ctggcgttcg atatgggcgg
    17401 tccggttaac aaagtggcct atgccttcat gctgatttgc gttgctcagg gtgtttatac
    17461 cgtggtggcc attactgccg tgggtatttg tatcccgccg ctggggatgg ggctggcgac
    17521 gctgattggt cgtaaaaatt tctccgcaga agagcgcgaa accggcaaag cggcgctggt
    17581 gatggggtgc gttggggtta ctgaaggggc gattcctttc gccgctgccg atccgctgcg
    17641 tgtcattcct tccatcatgg tcggttctgt ttgtggtgcg gtaactgcgg cgctggtcgg
    17701 tgcgcagtgc tatgcaggct ggggtggtct gattgtgctg ccggtggttg aaggcaagct
    17761 gggttatatc gcagcagtgg ctgtcggagc agtggtgacg gctgtttgcg tgaacgtgct
    17821 gaaaagtctg gcgcgtaaaa atgggtcttc gactgatgag aaggaagacg acctggattt
    17881 ggattttgaa attaattaat tgaggaacga gccatgacga aaattattgc agtaaccgcg
    17941 tgtccatctg gtgttgccca tacctatatg gcggcagagg cgctggaaag cgccgcgaaa
    18001 gcgaaaggct gggaagtgaa agtagaaact caggggtcaa ttggtctgga aaacgaactg
    18061 actgcggaag acgtggcgag cgccgatatg gtgattctga ccaaagatat cggcatcaag
    18121 tttgaagagc gttttgctgg caagaccatc gtgcgcgtca acatcagcga tgcggtaaaa
    18181 cgtgctgacg ccattatgag caaaattgaa gctcacctgg cgcaaaccgc ttaagttctt
    18241 ctcccgctcg caagggcggg ttcgctttcc cacaggagtt cctcatgacg aatcgtatct
    18301 ctcgcctcaa aactgcactg ttttccaata cccgtgaaat ctcgctggag cgggcgctgc
    18361 tttataccgc cagccatcgg caaaccgaag gcgaaccggt gatattgcgc cgggcgaaag
    18421 caacagcgta tatccttgaa catgttgaaa tttcgattcg tgatgaagaa ctgattgccg
    18481 gtaaccgcac cgtaaaaccg cgcgccggga ttatgtcgcc ggaaatggac ccttactggc
    18541 tgctgaaaga gctggatcaa ttcccgacgc gtccgcagga ccgctttgct atcagcgaag
    18601 aagataaacg tatctaccgc gaagagttgt tcccgtactg ggaaaaacgt tcgatgaaag
    18661 atttcatcaa cgggcagatg acagatgaag taaaagccgc gaccagcacg cagattttca
    18721 gcattaacca gacggataaa ggccaggggc acattattat tgattaccca cgcctgctga
    18781 atcacgggct gggggagctg gtggcgcaga tgcagcaaca ttgtcagcaa cagccggaga
    18841 atcactttta tcaggccgcg ctgttactgc tggaagcctc gcagaaacac attctgcgtt
    18901 acgctgaact ggcggaaaca atggcggcaa actgcacaga tgcccagcgt cgcgaagaac
    18961 tgctgactat tgcggagatc tcccgccata acgccgaaca taagccgcag acgttctggc
    19021 aggcgtgcca gttattctgg tacatgaaca tcattctgca atacgaatcc aacgccagtt
    19081 cgctgtcttt agggcgcttc gaccagtata tgttgccgtt ctatcaggct tctttaaccc
    19141 agggcgaaga tccggcgttc ctgaaagaac tgctcgaatc tttatgggtg aaatgcaacg
    19201 acatcgtgct gttgcgctcc accagcagcg cgcgttattt cgcaggtttc ccgaccggct
    19261 ataccgcact gctcggcggg ttaaccgaga acggacgtag cgcggtgaac gtgctttcgt
    19321 tcctttgcct tgacgcctat caaagcgtgc aattaccgca accgaacctc ggcgtgcgca
    19381 ctaacgcctt gatcgacacg ccgttcctga tgaaaaccgc cgaaaccatt cgcctcggca
    19441 ccggtattcc gcaaatcttt aacgatgaag tggtggtacc agcgttcctc aaccgtggcg
    19501 tttcgctgga agatgcgcgc gactattccg tagtgggctg tgtggaatta tctattcccg
    19561 gcagaaccta cggcttgcat gacatcgcga tgtttaatct gctgaaagtg atggaaatct
    19621 gcctgcatga aaatgaaggc aatgctgcgc tgacttatga aggtttactg gagcagatcc
    19681 gcgccaagat cagccactac atcaccctga tggttgaggg cagcaatatt tgtgatatcg
    19741 gccatcgcga ctgggcacct gtaccgctgc tctcgtcttt tatcagcgat tgtctggaaa
    19801 aaggtcgcga tattaccgat ggcggcgcgc gttataactt ctccggcgta caggggatcg
    19861 gtatcgccaa cctgagcgat tctctccatg cgttgaaagg gatggttttt gagcaacagc
    19921 gtttaagttt tgacgaattg ctgtcggtat taaaagccaa ctttgcaacg ccagaaggcg
    19981 aaaaagtccg tgctcgctta attaaccgct tcgagaaata cggtaacgat atcgacgagg
    20041 tggataacat cagcgccgaa ctgttacgcc actactgcaa agaagtggaa aaataccaga
    20101 acccgcgcgg tggttacttc acgccgggat cgtataccgt ttctgctcac gtcccgttgg
    20161 gatcggtggt tggcgcgacg ccagacggtc gttttgccgg ggaacaactg gcagacggcg
    20221 gcttgtcacc tatgctgggc caggacgcac aagggccaac agcggtactg aagtcagtca
    20281 gtaagctcga taatacgctg ctgtctaacg gtacgttgct gaacgtgaaa ttcactccgg
    20341 cgaccctgga aggtgaagcg ggattacgca aactggccga cttcttacgg gcgtttaccc
    20401 agcttaagtt gcagcatatt cagtttaacg tggtgaacgc cgacacgttg cgggaagcgc
    20461 aacagcgccc acaagattat gccgggctgg tggtgcgcgt tgccggatac agcgccttct
    20521 ttgtcgaact gtcgaaggag atccaggatg acatcatccg ccggacagcg catcagctgt
    20581 aacgttgtgg aaacgcgccg caatgatgtg gcgcgcattt tcaacattca gcgttattca
    20641 ctgaatgacg gcgagggcat tcgtacggtg gtctttttta aaggctgtcc gcatctttgc
    20701 ccatggtgtg ctaatccgga gtcgatctcc ggcaaaatcc agacggtacg cagagaggcg
    20761 aaatgtctgc actgtgcgaa atgtttgcgt gatgcggatg aatgcccctc cggggcgttt
    20821 gaacggattg gtcgcgatat cagccttgac gcgctggaac gggaagtgat gaaagatgac
    20881 attttctttc gcacgtccgg cggcggcgtc acgctttctg gcggcgaagt gttaatgcag
    20941 gcggagtttg ctacccgttt tttacagcgg ctgcggttgt ggggcgtgtc atgcgccatt
    21001 gaaactgccg gagacgcgcc agccagcaag ctgttaccac tggcgaaatt gtgcgatgaa
    21061 gtgctgttcg atttaaaaat tatggacgcg actcaggcgc gggatgtggt gaagatgaac
    21121 ctgccacgcg tgctggagaa tctgcgtttg ctggtgagtg agggtgtcaa cgtgatcccg
    21181 cgtttaccgt tgatccctgg tttcacgctc agccgggaga atatgcagca ggcgctggat
    21241 gtactgatcc cgctgaaaat caggcagatc catctgttac cgtttcatca gtacggcgaa
    21301 ccgaaatacc gcctgctggg gaaaacatgg tcgatgaaag aggtgcctgc gccgtcgtca
    21361 gccgatgtga caacgatgcg cgaaatggca gaacgggccg gatttcaggt taccgtggga
    21421 ggttaaaatg gcatacctgg tggcagtaac cgcctgcgta agcggcgtgg cgcatactta
    21481 tatggcggcg gaacggctgg aaaagttgtg ccagttagag aagtggggcg ttagcattga
    21541 aactcagggc gcgctgggaa cggagaatcg tttagcggac gaggatatcc gtcgggcgga
    21601 tgttgctctg ttgattacgg atatcgagct tgccggtgcc gagcgatttg aacattgccg
    21661 ctatgtgcaa tgcagcatct acgcattcct gcgtgagccg cagcgggtaa tgagcgcggt
    21721 gcgcaaagtg ctttctgcgc cgcagcaaac ccatcttatt ctggagtagt cggtttttct
    21781 gtcagttggc tgtggtactg ccgacgatat tccgacggcg agcgttcggt gtttttacga
    21841 aacagacggc agaagtagtt gctgtcgaca aagccgcagg cgtgcgccac ttcttttacc
    21901 ttcaggtcat agcctttcaa taacgtctta gcgtgctcca gtcgcgtgtg attcaggtat
    21961 tcgttaaaac caatggcccc cgttttttga aacaggtgcg agagatagtt tggtgaaata
    22021 tagaatgcct gtgcgacaga ttcgcgggta agcgcggagg catagcgttc gtcgatataa
    22081 tcgcgaatag cttcaaatag tgcctggctg cgtgaggcgg tctggatttg gctgccgagc
    22141 aaatcgcggc agtggctaag caggctggcg acgataagcc ttgccgtttg ctgctcctgc
    22201 ggctgcattt gcatttcatt gagcgtttgt agcagaaaag aacctatgcg tgggccgcgc
    22261 cgggcgacgt gttgcttcgc cagattttga tattgtttgc catcccattg cacgacgctg
    22321 aagccgagtt gctgtttgcc aaacagcacg ctaaaggtag tagcgggggc ttgccattgt
    22381 ggaaaattcc agccaccagc aggaacgtac agcacatcgc cgggaactaa cgttgctcca
    22441 gcgccggtat caacaaactc accttccagc acaatttcca gccgtggaaa atcgacctga
    22501 tacgcgagat cgggcaccgg gccgttactg ctggcaaagt aaatctggcg cagggacagc
    22561 gggccgttga tcaggcggga gagcagatag ctgacgtcgt gatacatgtt attcccttag
    22621 gtatgtagac gggaaaacag taactggcat tattaaaaaa ggccacctga aaagtgtggc
    22681 ctgaggggag ttcgatgtaa acattgcggc agtatcgcat ccggcaaata gcgccagacg
    22741 cgatacaaag gtcatttact gattacccac ctgatcgcca tacggcagcg tgtcgtaatc
    22801 gatcagcgcg tttttcttgt acgggttacc aatccagcgg gtagtttctt tgaactgcgg
    22861 attcaccact gaacgggttg gatcgtaacc gtcgtaagat aagcctgcta aatctgacca
    22921 ggtgtggatc agttccgcca ggctgtattt acgatcaaca tcctgcgaga aatcacgcgg
    22981 atgagtcgct tgccattttt ccgacgtcca cagcaggaac ggaatggtgt acatgtggcg
    23041 cgtcgggttg tcttcgttac gcccctgagt tttgtgcggc ggcgtgtcgt aaacctcttc
    23101 accgtggtca gagaaataaa ccaggaaacc gttcgggttt gctgctttaa agtctttaat
    23161 caggctggca accacatgat cgttatacag gttagcgttg tcataatcgt tatatgactc
    23221 cagctcttcc gcgtttaatc caggcggaac atgatcggta ttgccatcaa acttgccctg
    23281 attttccggg tagcggtatt tgtatttgat atgcgtaccc agcagatgaa cgatgatcag
    23341 tttcttcggc gccgggtcct tcagcacttc ctggaatggt ttcagcacgt tggtgtcgta
    23401 ttcacgcgca ctctgcgtac gttgctggtt catatagaac tgtttgtcgg tctgcttcga
    23461 aaataccgtc agcatggtat tgcgggcggt catcgtctgc tggttggtga tccagaaggt
    23521 tttataacct gcctgtttca tcatgttcat cagcgacggt tgtgtcaggt acaggtccgg
    23581 gttcttttca ttagcaaagg tcagcgcctg ttgcaggatt tcaatggtgt acggacgaga
    23641 cgtaaccacg ttattaaaca cggtcagatt cggatcggtt ttatgcagcg catccagctc
    23701 cggcgtggtt tcacgcggat aaccgtacag actcatgcgc ccgcgttggg tcgattcgcc
    23761 aatcaccagc actaaggtac gcggttcgtt acccgattca tctttgaaat tagccagagg
    23821 cggcaaggca ttattttcat tcagtaactt tgttagcgag tttagttgct gacgatactg
    23881 ataatagccg gtcaggaatt gccacggtgc ggcaggctcc atacgcgagg ccaggttatc
    23941 caacgttttc tcaaacggct tgtttttgat aaacgtattc atggcgatcg gatgcagaat
    24001 caagccataa agcagggcaa aagagacaac ataacgccac ggctttggaa tatagaccgg
    24061 gtgcaggcgt gtccacagca gaactgccac cgccgtatag gccagcgcga taagcacaat
    24121 tttcaggctg aaatactggc ttaaatactc gctggcttcg ttggtgttgg tttcgaacat
    24181 cacaaacaga acgctctgcg agaactcctg cccgtagatg acgtagtagc atagcgccgc
    24241 cagagaggcc gcccatagca ccacgccgat tactgcggca ataattttaa tccgcttcgg
    24301 aaagaggaat accgggatca accacagcga gctgaataac agcgagtcgc gaatgccgtt
    24361 ggtgccacta taaccactga tgtaaataat ggcctgtagc agagtagaga aaaaccaaaa
    24421 gtagagcagt gcccaaccca gggctttcca gctaaaaagg ggtttagcct ggacttctgt
    24481 ggaatgcata gtaagaacct gtcttgaaaa aaatatcgcc gaatgtaacg acaattcctt
    24541 aaggatatct gaaggtatat tcagaatttg aataaaatgc agacagaaat atattgaaaa
    24601 cgagggtgtt agaacagaag tatttcagaa aaccctcgcg caaaagcacg agggtttgca
    24661 gaagaggaag attagccggt attacgcata cctgccgcaa tcccggcaat agtgaccatt
    24721 aacgcttgtt cgacgcgagg atccggttcc tggccttctt tttctgcctg gcgggagcgg
    24781 tgcagtaact cggcctgcaa tacgttcagc gggtcggtgt aaatattccg tagctgaata
    24841 gactctgcaa tccacggcag atcggccatt agatgggaat cgttggcaat cgccagcacc
    24901 actttgatgt cttcttcttg caggttgcgt aactctttac ctaacggcca cagtgctttg
    24961 tctaccaggc gttggtcata gtattccgcc agccacaggt ctgctttggc gaagaccatc
    25021 tccagcatgc cgagacgcgt cgagaagaat ggccaatcgc ggcacatggc ttccagctcg
    25081 ctctgtttgc catcttcgac cactttttgc agcgccgtac ctgcacccag ccaggcgggg
    25141 agcatcaggc ggttttgcgt ccaggcaaaa atccacggta tggcgcgcag cgactcgacg
    25201 ccgccggttg ggcgacgttt cgccggacgt gaacccaacg gcagtttgcc caattcttgt
    25261 tccggcgtag cggagcggaa gtaaggcaca aaatctttgt tttcacgtac gtagccacgg
    25321 tagagatcgc aggagatgac tgacagttca tccataatgc gacgccagct ctctttcggc
    25381 tcaggtggtg gcagcaggtt ggcttccaga atagccccgg tgtacagcga caggctgctg
    25441 acggtgattt ctggcagacc atatttaaag cggatcatct cgccctgttc agtcacgcgc
    25501 aggccacctt tcaggcttcc tggcggttgt gacagcagcg ccgcatgagc aggtgcgccg
    25561 ccgcgaccaa tggaaccgcc gcgaccgtgg aacaacgtca gctcaatacc cgctttttcg
    25621 caggttttga ttaatgcatc ctgtgcctga tattgcgccc aggaagctgc catcactccc
    25681 gcatcttttg ctgagtcgga atagccaatc atcaccatct gtttgccctg aatcaggcca
    25741 cgataccagt caatattgag cagctgggtc atgacatcgt tggcgttgtt cagatcatcg
    25801 agggtttcaa acagcggagc aaccggcatc gcaaacccga tacccgcttc tttcagcagc
    25861 aggtggacag ccagtacgtc ggacggcgtt ttcgccatcg agatcacgta ggcggcaatg
    25921 gagccttgcg gtgcttcggc aatcacctgg caggtatcga gcacttcgcg cgtttcggcg
    25981 cttggttgcc agttgcgcgg cagaagcgga cgtttggagt tcagttcgcg gatcaggaac
    26041 gcctgtttgt cggcctctga ccagctttcg tagtcgccga taccgaggta gcgggtcagc
    26101 tcgcccagcg cttcggtatg acgcgtgctc tcctgacgga tatcaatacg gaccagcggt
    26161 acgccgaaac atttcacgcg gcgcagggtg tcgagcagat cgccgttggc gataataccc
    26221 atgccacacg cctgaagtga ctggtagcaa gcgtagagcg gttcccacag ttcttcgttt
    26281 tgtgtcagca ggccttctgg ttttggcagt tcttcgcctt tcaggcgcgc ttccagccat
    26341 gcctgtgtcg ccatcaggcg agaacgcagg tttttcatca gatagcgata cggttctgcg
    26401 gcaccttctt cgccaaccag cgccagcagt tcaggggtcg cttcaaccat cgacagttca
    26461 gaaaccagca cctgaatatc tttcaggaac aaatcggtgg ctttccagcg gctgagtagc
    26521 aggacgtggc gggtgatatc ggcagtgacg ttcgggttgc cgtcgcggtc gccgcccatc
    26581 cacgaagtaa aacggaccgg aacaaattcg acgggcagtt tgtagccgag gttctcttcc
    26641 agttgttcgt tcagttcgcg caggtaattt ggtacgcctt gccacaggct gttttccact
    26701 acggcaaagc cccatttggc ttcatctacc gggcttggac gcagcttacg gatttcatcg
    26761 gtatgccatg actgggcgat taactggcgc aggcggcgca tcagctggtt gtgttcgtag
    26821 tcagcgatat ctttgttatc gagctgtttt aaacaggcgt tcacttccac cattttgtgg
    26881 atcagtgtac gacgggtaat ttcggttggg tgagccgtga ggaccagttc cagcgacagc
    26941 gattccactg cttttttgat ggtgtcttcg ctcagttccg gctggttttt cagtttacgc
    27001 agggtgcggg cgatcacttc cgggttgctg gcagcttcgc ctttcggcga aatgctgtgg
    27061 tattgctcgg cggtgttggc caggttcagg aactgactaa acgcacgcgc aacgggcagc
    27121 agctcgtcgt tcgacaaatt ttgtaaggtg gtgagcaact cctggcggtt agcatcattg
    27181 ccagcgcgtg aagatttcga caacttacgg atagtttcta cgcgttcaag aatgtgttct
    27241 cccaacgcat ccttgatggt ttctcccagc actttgccga gcatactgac attactacgc
    27301 aatgcggaat attgttcgtt catattaccc cagacacccc atcttatcgt ttgatagccc
    27361 tgtatccttc acgtcgcatt ggcgcgaata tgctcgggct ttgcttttcg tcgtctttta
    27421 taaagccacg taaaagcggt gacgtcaaat gctgcgaaat cgcttcagca aacgaataaa
    27481 tagcaggaat ttacgccatt aaattcacga cgctttaaat aagcgtaact tatggaaatg
    27541 ttaaaaaatc gccatacata acaccaaagg tgtaggtcgg ataagatgcg caagcatcgc
    27601 atccgacatt attgcggcac aggagtttgg caacggtgcc ggatgcggcg cgagcgcctt
    27661 atccggccta cgttttaatg ccagcaaaaa tggtgaatta cctgggttat cagttcgcgg
    27721 gtgggcttga taaaccgtgt ttccagatat tcatcaggtt gatgagcctg attaattgag
    27781 ccaggcccca acaccagcgt cgggcataac gtttgaataa acggcgcttc ggtacagtag
    27841 ttcaccactt cggtctttgc tccgagcaat ttctcaacta cttcaaccag ttgatgattc
    27901 ggtgggcatt catagccagg gatcggcgga tgcagctcgt cgaccgtcag acgacccggc
    27961 cagcgttcgc tcaccggagc caatgcatcg ttgagcaaac cattaagttc attgagtgtc
    28021 atgccaggca gcggacgaat atccatatgc aactcacagc aagcgcaaat acggttagaa
    28081 gcgtcgccac cgtgaatatg cccgaggttg agcgtagggt atggcacggt aaacgcttcg
    28141 tagtgataac gttctttcag gttatcgcgc aattgcaaaa tatgcccgat ggcgtcgtgc
    28201 attagttcga tagcgttaac tccgcgtgct ggatcgctgg agtgccccga ctggccctga
    28261 atacggatgg cgttagagat atgaccttta tgtgcgcgta ccggttgtag tgacgtcggt
    28321 tcgccaatga tggcgcaatc cgggcgcagg gcggtagttt cggcaaaata acgcgctccg
    28381 gccatactgg tttcttcatc agcagtcgcc agaatgtaga gcggtttttt cagtttcgtg
    28441 acgtcgacat cgcgtagcgc atcaaggata aacgcaaaaa agcctttcat gtcggcggtg
    28501 cctaagccgt aaagcttgcc gtcatgctcc gtcagtgtaa acggatcgcg cgtccagcga
    28561 ccgtcatcaa atggcaccgt atcggtatgc cccgccagca acaagccgcc agccccctgt
    28621 ccgatacttg ccagcatatt gaatttgttg cgagttcctg gcacaggctg cacttccaca
    28681 ttgaagccta aatctttaaa ccagtccgcc agcagagtga ttaaatctgc attgctttga
    28741 tcgagtgcct cttccgtggc gcttattgaa ggtgtggcaa tcagagcacg gtaaatctcg
    28801 ataaatggcg gtaatttgtt tttcattgtt gacacacctc tggtcatgat ggtatcaata
    28861 ttcatgcagt atttatgaat aaaaatacac taacgttgag cgtaataaaa cccaccagcc
    28921 gtaaggtgaa tgttttacgt ttaacctggc aaccagacat aagaaggtga atagccccga
    28981 tgttgaatac gctgattgtg ggtgccagcg gctacgctgg cgcagagcta gtgacctatg
    29041 taaatcgcca tccgcatatg aacataaccg ctttgactgt ttcagcgcaa agcaatgatg
    29101 cgggaaagtt aatctccgat ttgcatccgc agctaaaagg catcgttgat ctgccgttgc
    29161 agccgatgtc ggatatcagc gagtttagcc caggggtgga cgtagtgttt ctcgccaccg
    29221 cccatgaagt tagccacgat ttagcgccgc agtttcttga agcgggctgc gtggtgttcg
    29281 acctttccgg cgcgtttcgt gttaacgacg ccaccttcta tgaaaaatat tacggcttta
    29341 cccatcaata cccggaactg ttggaacagg cagcctacgg tctggcggag tggtgcggta
    29401 ataaattaaa agaagcgaat ttgattgcgg tgccgggctg ttatccgacg gcggcacagc
    29461 tggcgctgaa accgttgatt gatgccgatc ttcttgacct caatcagtgg ccggtgatca
    29521 acgccaccag cggcgtgagc ggtgcaggac gtaaggcagc gatttcaaat agtttttgtg
    29581 aagttagcct gcaaccgtat ggcgtcttta cccatcgcca tcaaccagag atcgccacac
    29641 acctcggtgc tgacgttatc ttcaccccgc atctgggcaa tttcccgcgc ggcattcttg
    29701 aaaccattac ctgccgcctg aaaccgggtg tgacccaggc gcaagtggcg caagcgttac
    29761 agcaggcgta tgcccataaa ccgctggtgc ggctgtatga caaaggcgtt ccggcgctga
    29821 aaaatgtcgt tgggctgcca tgttgcgata tcgggtttgc cgttcagggc gagcatctga
    29881 ttattgtggc gaccgaagac aacttactga aaggcgcggc ggcacaagcg gtacagtgcg
    29941 ccaatattcg tttcggctat gcggaaacgc agtctcttat ttaagggtgc aatgatgaat
    30001 ccattaatta tcaaactggg cggcgtactg ctggatagtg aagaggcgct ggaacgtctg
    30061 tttagcgcac tggtgaatta tcgtgagtca catcagcgtc cgctggtgat tgtgcacggc
    30121 ggcggttgcg tggtggatga gctgatgaaa gggctgaatc tgccggtgaa aaagaaaaat
    30181 ggcctgcggg tgacgcctgc ggatcagata gacattatca ccggagcact ggcaggaacg
    30241 gcaaataaaa ccctgctggc atgggcgaag aaacatcaga ttgcggccgt aggtttgttt
    30301 ctcggtgacg gcgacagcgt caaagtgacc cagcttgatg aagagttagg tcatgttgga
    30361 ctggcgcagc caggttcgcc taagcttatc aacaccttgc tggagaacgg ctatctgccg
    30421 gtggtcagct ccattggcgt aacagacgaa gggcaactga tgaacgtcaa tgccgaccag
    30481 gcggcgacgg cgctggcggc aacgctgggc gcggatctga ttttgctctc tgatgtcagc
    30541 ggcattctcg acggcaaagg gcaacgcatt gccgaaatga ccgccgcgaa agcagaacaa
    30601 ctgattgagc agggcattat tactgacggc atgatagtga aagtgaacgc ggcgctggat
    30661 gctgcgcgca cgttgggccg tccggtagat atcgcctcct ggcgtcatgc ggaacagctt
    30721 ccggcactgt ttaacggtat gccgatgggt acgcggattt tagcttaagt tttgttggcc
    30781 ggaggcgcag ctttccggca ttgaatttca aaataaggaa acagagttat ggcactttgg
    30841 ggcgggcgtt ttacccaggc agcagatcaa cggttcaaac aattcaacga ctcactgcgc
    30901 tttgattacc gtctggcgga gcaggatatt gttggctctg tggcctggtc caaagccctg
    30961 gtaacggtcg gcgtgttaac cgcagaagag caggcgcaac tggaagaggc gctgaacgtg
    31021 ttgctggaag atgttcgcgc caggccacaa caaatccttg aaagcgacgc cgaagatatc
    31081 catagctggg tggaaggcaa actgatcgac aaagtgggcc agttaggcaa aaagctgcat
    31141 accgggcgta gccgtaatga tcaggtagcg acagacctga aactgtggtg caaagatacc
    31201 gttggcgagt tgctgacggc taaccggcag ctgcaatcgg cgctggtgga aaccgcacaa
    31261 aacaatcagg acgcggtgat gccaggttac actcacctgc aacgcgccca gccggtgacg
    31321 ttcgcgcact ggtgcctggc ctatgttgag atgctggcgc gtgatgaaag ccgtttgcag
    31381 gatgcgctta agcgtctgga tgtcagccca ttaggctgtg gcgcgctggc gggaacggcc
    31441 tatgaaatcg accgtgaaca gttagcaggc tggctgggct ttgcttcggc gacccgtaac
    31501 agtctcgaca gcgtttctga ccgtgaccat gtgttggaac tgctttctgc tgccgctatc
    31561 ggcatggtgc atctgtcgcg ttttgctgaa gatctgattt tctttaacac cggcgaagcg
    31621 gggtttgtgg agctttctga ccgcgtgact tccggttcat cattaatgcc gcagaagaaa
    31681 aacccggatg cgctggagct gattcgcggt aaatgcggtc gggtgcaggg ggcgttaacc
    31741 ggcatgatga tgacgctgaa aggtttgccg ctggcttaca acaaagatat gcaggaagac
    31801 aaagaaggtc tgttcgacgc gctcgatacc tggctggact gcctgcatat ggcggcgctg
    31861 gtgctggacg gcattcaggt gaaacgtcca cgttgccagg aagcggctca gcagggttac
    31921 gccaacgcca ccgaactggc ggattatctg gtggcgaaag gcgtaccgtt ccgcgaggcg
    31981 caccatattg ttggtgaagc ggtggtggaa gccattcgtc agggcaaacc gctggaagat
    32041 ctgccgctcg acgagttgca gaaattcagc ccggtgattg acgaagatgt ctatccgatt
    32101 ctgtcgctgc aatcgtgcct cgacaagcgt gcggcaaaag gcggcgtctc accgcagcag
    32161 gtggcgcagg caattgcttt tgcgcaggcg cggttggggt aagaacattt atatgtataa
    32221 atttgagcct ggcttatcgc cgggcttttt tatggcaaaa aaaagcggat cctggagatc
    32281 cgcaaaagtt cacgttggct ttagttattc gagttgagaa actctcgaaa cgggcagtga
    32341 cttcaagggt taaaagaggt gccgctccgt ttctgtgagc aattatcagt cagaatgctt
    32401 gatagggata atcgttcatt gctattctac ctatcgccat gaactatcgt ggcaatggag
    32461 gatggataat gaatattcgt gatcttgagt acctggtggc attggccgaa caccgccatt
    32521 ttcggcgtgc ggcagattcc tgccacgtta gccagccgac gcttagcggg caaattcgta
    32581 agctggaaga tgagttgggc gtgatgttgc tggagcggac cagccgtaaa gtgttgttca
    32641 cccaggcggg aatgctgctg gtagatcagg cgcgtaccgt actgcgtgag gtgaaagtcc
    32701 ttaaagagat ggcaagccag cagggcgaga cgatgtccgg gccgctgcac attggtttga
    32761 ttcccacagt tggaccgtac ctgctaccgc atattatccc tatgctgcac cagacctttc
    32821 caaagctgga aatgtatctg catgaagcac agacccacca gttactggcg caactggaca
    32881 gcggcaaact cgattgcgtg atcctcgcgc tggtgaaaga gagcgaagca ttcattgaag
    32941 tgccgttgtt tgatgagcca atgttgctgg ctatctatga agatcacccg tgggcgaacc
    33001 gcgaatgcgt accgatggcc gatctggcag gggaaaaact gctgatgctg gaagatggtc
    33061 actgtttgcg cgatcaggca atgggtttct gttttgaagc cggggcggat gaagatacac
    33121 acttccgcgc gaccagcctg gagacgctgc gcaacatggt ggcggcaggt agcgggatca
    33181 ctttactgcc tgcgctggct gtgccgccgg agcgcaaacg cgatggggtt gtttatctgc
    33241 cgtgcattaa gccggaacca cgccgcacta ttggcctggt ttatcgtcct ggctcaccgc
    33301 tgcgcagccg ctatgagcag ctggcagagg ccatccgcgc acgaatggat ggccatttcg
    33361 ataaagtttt aaaacaggcg gtttaaaccg tttaacgcag ctacccgata ggcttccgcc
    33421 atcgtcgggt agttaaaggt ggtgttgacg aagtactcaa tagtgttgcc gccacctttc
    33481 tgttccataa tcgcctgacc gatatgaata atttcggcag cgcgctcgcc aaagcagtga
    33541 atacccagaa tctcttttgt ttcccgatgg aacaaaattt tcagcgtgcc cacgttcatg
    33601 ccgacgattt gtgcgcgtgc cagatgttta aactgggcgc ggcccacttc atatggcact
    33661 ttcattgcgg tcagctgctg ttcggttttg cccacagagc tgatttccgg gatggtgtaa
    33721 atgccggtag ggatatcttc aatcagatgt gcggtggctt cgccttttac cagcgcctgc
    33781 gcggcaatgc gcccctggtc ataggccgct gatgccaggc tcggataacc aatcacgtcg
    33841 cccaccgcgt aaacgtgcgg ctgtgcggtc tgatacatgc tgttgacctt cagctgtccg
    33901 cggctgtcag tttccagccc aatgttctgt aacgccagcg aatcggtatt accggtgcga
    33961 ccgttggcat agagcaggca gtcagctttc agttttttac ccgacttcaa atgcatgatc
    34021 acaccatcgt cacagccttc gatcttctcg tactcttcgt tgtgacgaat cactacgcca
    34081 ctgttccaga agtgatagga gagagaatct gacatctctt gatcgagaaa tgccagcagg
    34141 cgatcgcggg tgttgatcag atccactttt acatccatac cgcggaagat cgacgcatat
    34201 tcacagccga tcactccagc accatagata agtacatggc gcggttcgtg gtgcatgcta
    34261 agaattgagt cgctgtcgta aatgcgtgga tgggtgaaat caacatctgt tggatgatat
    34321 ggacgagagc cgcaggcaat aacaaatttt tcagcggtta gtgtttcaac gctgccgtcc
    34381 gggcaatcca gcgccaacgt atgctcgtca acaaagcgag cgtttccctg caatatttca
    34441 cagtgattac gttcgtaaaa tccctgacgc atgcgcgttt gttgattaat cacgttatcg
    34501 gcatggttaa ggatatcggc aaaagaagag cggagcagtc gggaatggtc gctgtaaagt
    34561 gggttttgat tgaattctat aatgcggctg acggcgtgac ggagagcttt cgacgggatg
    34621 gtgccccagt gggtgcaacc gccgccaaca ttttgataac gctcgataac tgcgacgcgc
    34681 gcaccttgct taaccaggcc cattgcagcg ccttcgccgc cggggccgga acctattact
    34741 atggcatcgt aatcgtagga atgtggcatg gtagggctta cctgttctta tacataaaag
    34801 caacagaatg gtaacatttt atcgcgggta agccaattga tccccgtcat ttatctggct
    34861 atatcctgag cggcctttgc tttgtctgtt tcttactttt gccctgacgt tttattggat
    34921 ttttatcgac gatactctcc gtttaagcgg caggtttccg ctgtacgtaa aagaaccggc
    34981 caaagaattg cagtaaatat gttttattgc gttaccgttc attcacaata ctggagcaat
    35041 ccagtatgtt cattctctgg tatagtgcca gcagtacttt tggcaaggat tcagacatcg
    35101 tgatgggcgt aagagcgcaa caaaaagaaa aaacccgccg ttcgctggtg gaagccgcat
    35161 ttagccaatt aagtgctgaa cgcagcttcg ccagcctgag tttgcgtgaa gtggcgcgtg
    35221 aagcgggcat tgctcccacc tctttttatc ggcatttccg cgacgtagac gaactgggtc
    35281 tgaccatggt tgatgagagc ggtttaatgc tacgccaact catgcgccag gcgcgtcagc
    35341 gtatcgccaa aggcgggagt gtgatccgca cctcggtctc cacatttatg gagttcatcg
    35401 gtaataatcc taacgccttc cggttattat tgcgggaacg ctccggcacc tccgctgcgt
    35461 ttcgtgccgc cgttgcgcgt gaaattcagc acttcattgc ggaacttgcg gactatctgg
    35521 aactcgaaaa ccatatgccg cgtgcgttta ctgaagcgca agccgaagca atggtgacaa
    35581 ttgtcttcag tgcgggtgcc gaggcgttgg acgtcggcgt cgaacaacgt cggcaattag
    35641 aagagcgact ggtactgcaa ctgcgaatga tttcgaaagg ggcttattac tggtatcgcc
    35701 gtgaacaaga gaaaaccgca attattccgg gaaatgtgaa ggacgagtaa tgaaacaagc
    35761 aaatcaagat agaggtacgc tgctgctggc gttagttgct ggcttatcga ttaatggtac
    35821 tttcgcagcg ctgtttagct ccattgtgcc attttctgta ttcccgatta tttccctggt
    35881 gctgacggtt tacagcctgc atcaacgtta tcttaatcgc actatgccgg taggcttgcc
    35941 gggtctggca gctgcctgtt ttattctcgg cgtactgctg tacagcacgg tagttcgtgc
    36001 ggaatatccg gatatcggct ctaacttctt cccggcagta ctctccgtca ttatggtgtt
    36061 ctggattggc gcgaagatgc gtaaccgtaa gcaggaagtt gctgaataat cggtattatg
    36121 ccggatgctg gcgcatccag catgggtttt acttcgcggt cagtaatacg ccgcactcca
    36181 tatggtgcgt gtaggggaac tgatcaaaca gagccagacg ttcgaccttg tgcgtctggc
    36241 ttaatgtttc cagattcttg cataacgttt ccgggttaca ggagatgtac aaaatacacg
    36301 gatacgcctg caccattttc tcggtttcac tgtccagacc gctgcgcggt gggtcgacaa
    36361 aaatggtttc gcactgataa ctctttaagt cgatcccttg caggcggtta aactcgcgta
    36421 caccattcat cgcctgagta aattcttctg ccgccatacg aataatttgt acgttatcaa
    36481 tatggttagc tgcgatgttg tattgcgcag cagcaaccga cggcttagcg atttcggtgg
    36541 ctaatacccg atcaaaattg cgcgccagcg ctaatgaaaa gttaccgttg ccgcagtaca
    36601 gctccagtaa atcgcctttt gagcctttgg ttacgtccag cgcccattcc agcatctgaa
    36661 tattcatcgc cgcgttcggc tgggtaaagc tgttttctac ctgacggtag atcatctctt
    36721 tccctgcgac cggcagacgc tcgtcgatgt aatcttgatc cagctcgatt ttggttttcg
    36781 ttgcccgacc aatcagatgc acattcagat tctgcgcgcg cagtgcatcg cgcagggcct
    36841 ccgcttcctg acgccactca tcatccagct tcttatggta tagcagggaa accaccgcct
    36901 gattactcag cgtggtgagg taatcaatct ggaacaactt gtggcgcaga acgggattat
    36961 tacgcacacc cgcaatcatc gccgtcatca actggttgat aagttcactg gcggcgggga
    37021 agctatccac gcggatgcgg cttttggttt gttgatcgaa aatgatgtga tacaggtcat
    37081 cgccatcgtg ccagatgcgg aactccgcgc gcatccggta atgactgacc ggcgagcgaa
    37141 acacttccgg aaccaggtca gaaaacggtg ccatcatact ttgcaaacgt accacttttt
    37201 cggctaactg cgcttcatac tgttctgttg gaaggtgttc gggggtcatg atgtatcctg
    37261 aaaaattaaa gtacgcggcg attgtaggga ttgctcatca gatgtccaga tcttgatgaa
    37321 ttcctatttg tgagctacgt ctggacagta acttgttaca acctgtagca tccacttgcc
    37381 ggtcctgtga gttaataggg aatccagtgc gaatctggag ctgacgcgca gcggtaagga
    37441 aaggtgcgat gattgcgtta tgcggacact gccatccggt gggaagtcat catctcttag
    37501 tatcttagat acccctccaa gcccgaagac ctgccggcca acgtcgcatc tggttctcat
    37561 catcgcgtaa tattgatgaa acctgcggca tccttcttct attgtggatg ctttacaatg
    37621 attaaaaaag cttcgctgct gacggcgtgt tctgtcacag ccttttccgc ttgggcacag
    37681 gataccagcc cggatactct cgtcgttact gctaaccgtt ttgaacagcc gcgcagcact
    37741 gtgcttgcac caaccaccgt tgtgacccgt caggatatcg accgctggca gtcgacctcg
    37801 gtcaatgatg tgctgcgccg tcttccgggc gtcgatatca cccaaaacgg cggttcaggt
    37861 cagctctcat ctatttttat tcgcggtaca aatgccagtc atgtgttggt gttaattgat
    37921 ggcgtacgcc tgaatctggc gggggtgagt ggttctgccg accttagcca gttccctatt
    37981 gcgcttgtcc agcgtgttga atatatccgt gggccacgct ccgccgttta tggttccgat
    38041 gcaataggcg gggtggtgaa tatcatcacg acgcgcgatg aacccggaac ggaaatttca
    38101 gcagggtggg gaagcaatag ttatcagaac tatgatgtct ctacgcagca acaactgggg
    38161 gataagacac gggtaacgct gttgggcgat tatgcccata ctcatggtta tgatgttgtt
    38221 gcctatggta ataccggaac gcaagcgcag acagataacg atggtttttt aagtaaaacg
    38281 ctttatggcg cgctggagca taactttact gatgcctgga gcggctttgt gcgcggctat
    38341 ggctatgata accgtaccaa ttatgacgcg tattattctc ccggttcacc gttgctcgat
    38401 acccgtaaac tctatagcca aagttgggac gccgggctgc gctataacgg cgaactgatt
    38461 aaatcacaac tcattaccag ctatagccat agcaaagatt acaactacga tccccattat
    38521 ggtcgttatg attcgtcggc gacgctcgat gagatgaagc aatacaccgt ccagtgggca
    38581 aacaatgtca tcgttggtca cggtagtatt ggtgcgggtg tcgactggca gaaacagact
    38641 acgacgccgg gtacaggtta tgttgagaat ggatatgatc aacgtaatac cggcatctat
    38701 ctgaccgggc tgcaacaagt cggcgatttt acctttgaag gcgcagcacg cagtgacgat
    38761 aactcacagt ttggtcgtca tggaacctgg caaaccagcg ccggttggga attcatcgaa
    38821 ggttatcgct tcattgcttc ctacgggaca tcttataagg caccaaatct ggggcaactg
    38881 tatggcttct acggaaatcc gaatctggac ccggagaaaa gcaaacagtg ggaaggcgcg
    38941 tttgaaggct taaccgctgg ggtgaactgg cgtatttccg gatatcgtaa cgatgtcagt
    39001 gacttgatcg attatgatga tcacaccctg aaatattaca acgaagggaa agcgcggatt
    39061 aagggcgtcg aggcgaccgc caattttgat accggaccac tgacgcatac tgtgagttat
    39121 gattatgtcg atgcgcgcaa tgcgattacc gacacgccgt tgttacgccg tgctaaacag
    39181 caggtgaaat accagctcga ctggcagttg tatgacttcg actggggtat tacttatcag
    39241 tatttaggca ctcgctatga taaggattac tcatcttatc cttatcaaac cgttaaaatg
    39301 ggcggtgcga gcttgtggga tcttgcggtt gcgtatccgg tcacctctca tctgacagtt
    39361 cgtggtaaaa tagccaacct gttcgacaaa gattatgaga cagtctatgg ctaccaaact
    39421 gcaggacggg aatacacctt gtctggcagc tacaccttct gaaccacgtc ccaccgtgct
    39481 ggtgtttgac tccggcgtcg gtgggttgtc ggtctatgac gagatccggc atctcttacc
    39541 ggatctccat tacatttatg ctttcgataa cgtcgctttt ccgtatggcg aaaaaagcga
    39601 agagtttatt gttgagcgag tggtggcaat tgtcaccgca gtgcaagaac gttatcccct
    39661 tgcgctggct gtggtcgctt gcaacaccgc cagtaccgtt tcacttcctg cattacgcga
    39721 aaagttcgac ttcccggttg ttggtgtcgt gccggcgatt aaacctgctg cacgtctgac
    39781 ggcaaatggc attgtcggat tactggcaac ccgcggaact gttaaacgtt cttatactca
    39841 tgagctgatc gcgcgtttcg ctaatgaatg ccagatagaa atgctgggct cggcagagat
    39901 ggttgagttg gctgaagcga agcttcatgg cgaagatgtt tctctggatg cactaaaacg
    39961 tatcctacgc ccgtggttaa gaatgaaaga gccgccagat accgttgtat tgggttgcac
    40021 ccatttccct ctactacaag aagaactctt acaggtgctg ccagagggaa cccggctggt
    40081 ggattctggc gcagcgattg ctcgcagaac ggcctggctg ttagaacatg aagctccgga
    40141 tgcaaaatct gccgatgcga atattgcctt ttgtatggca atgacgccag aagctgaaca
    40201 attattgccc gttttacagc gttacggctt cgaaacgctc gaaaaactgg cagttttagg
    40261 ctgatttggt tgaatgttgc gcggtcagaa aattatttta aatttcctct tgtcaggccg
    40321 gaataactcc ctataatgcg ccaccactga cacggaacaa cggcaaacac gtcgccgggt
    40381 cagcggggtt ctcctgagaa tctcaacaga gaaaagcaaa aaatgcttga ctctgtagcg
    40441 ggaaagcgta ttatgcacac cccgcgccgc tgagaaaaag cgaagcgg
//
LOCUS       DAZGTR010000028        40231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGTR010000028 DAZGTR010000000
VERSION     DAZGTR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.028"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            28..981
                     /gene="ybhD"
                     /locus_tag="ACHLCY_001666"
     CDS             28..981
                     /gene="ybhD"
                     /locus_tag="ACHLCY_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0368953.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1022..2017)
                     /gene="pgl"
                     /locus_tag="ACHLCY_001667"
     CDS             complement(1022..2017)
                     /gene="pgl"
                     /locus_tag="ACHLCY_001667"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP0368954.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2172..2990
                     /gene="ybhA"
                     /locus_tag="ACHLCY_001668"
     CDS             2172..2990
                     /gene="ybhA"
                     /locus_tag="ACHLCY_001668"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP0368955.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     HHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(2991..4049)
                     /gene="modC"
                     /locus_tag="ACHLCY_001669"
     CDS             complement(2991..4049)
                     /gene="modC"
                     /locus_tag="ACHLCY_001669"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP0368956.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4052..4741)
                     /gene="modB"
                     /locus_tag="ACHLCY_001670"
     CDS             complement(4052..4741)
                     /gene="modB"
                     /locus_tag="ACHLCY_001670"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP0368957.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4741..5514)
                     /gene="modA"
                     /locus_tag="ACHLCY_001671"
     CDS             complement(4741..5514)
                     /gene="modA"
                     /locus_tag="ACHLCY_001671"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0368958.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEEHNNATVKAFYDYLKGSQAAEIFKRYGFTTK"
     gene            complement(5680..5829)
                     /gene="acrZ"
                     /locus_tag="ACHLCY_001672"
     CDS             complement(5680..5829)
                     /gene="acrZ"
                     /locus_tag="ACHLCY_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP0368959.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5958..6746
                     /gene="modE"
                     /locus_tag="ACHLCY_001673"
     CDS             5958..6746
                     /gene="modE"
                     /locus_tag="ACHLCY_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP0368960.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6814..8286
                     /gene="modF"
                     /locus_tag="ACHLCY_001674"
     CDS             6814..8286
                     /gene="modF"
                     /locus_tag="ACHLCY_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP0368961.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8547..9563
                     /gene="galE"
                     /locus_tag="ACHLCY_001675"
     CDS             8547..9563
                     /gene="galE"
                     /locus_tag="ACHLCY_001675"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0368962.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9573..10619
                     /gene="galT"
                     /locus_tag="ACHLCY_001676"
     CDS             9573..10619
                     /gene="galT"
                     /locus_tag="ACHLCY_001676"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP0368963.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10623..11771
                     /gene="galK"
                     /locus_tag="ACHLCY_001677"
     CDS             10623..11771
                     /gene="galK"
                     /locus_tag="ACHLCY_001677"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP0368964.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYYLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11765..12805
                     /gene="galM"
                     /locus_tag="ACHLCY_001678"
     CDS             11765..12805
                     /gene="galM"
                     /locus_tag="ACHLCY_001678"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP0368965.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13008..13760
                     /gene="gpmA"
                     /locus_tag="ACHLCY_001679"
     CDS             13008..13760
                     /gene="gpmA"
                     /locus_tag="ACHLCY_001679"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP0368966.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13919..14971)
                     /gene="aroG"
                     /locus_tag="ACHLCY_001680"
     CDS             complement(13919..14971)
                     /gene="aroG"
                     /locus_tag="ACHLCY_001680"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP0368967.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGETAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15287..15667
                     /gene="ybgS"
                     /locus_tag="ACHLCY_001681"
     CDS             15287..15667
                     /gene="ybgS"
                     /locus_tag="ACHLCY_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP0368968.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15781..16722
                     /gene="zitB"
                     /locus_tag="ACHLCY_001682"
     CDS             15781..16722
                     /gene="zitB"
                     /locus_tag="ACHLCY_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP0368969.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNKGVSGHSHHHH"
     gene            complement(16719..17438)
                     /gene="pnuC"
                     /locus_tag="ACHLCY_001683"
     CDS             complement(16719..17438)
                     /gene="pnuC"
                     /locus_tag="ACHLCY_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP0368970.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRLAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17476..18519)
                     /gene="nadA"
                     /locus_tag="ACHLCY_001684"
     CDS             complement(17476..18519)
                     /gene="nadA"
                     /locus_tag="ACHLCY_001684"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP0368971.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18768..18893)
                     /locus_tag="ACHLCY_001685"
     ncRNA           complement(18768..18893)
                     /ncRNA_class="other"
                     /locus_tag="ACHLCY_001685"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18925..19000)
                     /locus_tag="ACHLCY_001686"
     tRNA            complement(18925..19000)
                     /locus_tag="ACHLCY_001686"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18965..18967),aa:Lys,seq:ttt)
     gene            complement(19005..19080)
                     /locus_tag="ACHLCY_001687"
     tRNA            complement(19005..19080)
                     /locus_tag="ACHLCY_001687"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19045..19047),aa:Val,seq:tac)
     gene            complement(19125..19200)
                     /locus_tag="ACHLCY_001688"
     tRNA            complement(19125..19200)
                     /locus_tag="ACHLCY_001688"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19165..19167),aa:Val,seq:tac)
     gene            19459..20874
                     /gene="gltX"
                     /locus_tag="ACHLCY_001689"
     CDS             19459..20874
                     /gene="gltX"
                     /locus_tag="ACHLCY_001689"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP0368972.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(20926..21318)
                     /gene="yfeD"
                     /locus_tag="ACHLCY_001690"
     CDS             complement(20926..21318)
                     /gene="yfeD"
                     /locus_tag="ACHLCY_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0368973.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(21320..21679)
                     /gene="yfeC"
                     /locus_tag="ACHLCY_001691"
     CDS             complement(21320..21679)
                     /gene="yfeC"
                     /locus_tag="ACHLCY_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0368974.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            21900..21975
                     /locus_tag="ACHLCY_001692"
     tRNA            21900..21975
                     /locus_tag="ACHLCY_001692"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21933..21935,aa:Ala,seq:ggc)
     gene            22015..22090
                     /locus_tag="ACHLCY_001693"
     tRNA            22015..22090
                     /locus_tag="ACHLCY_001693"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22048..22050,aa:Ala,seq:ggc)
     gene            22298..24487
                     /gene="pdeA"
                     /locus_tag="ACHLCY_001694"
     CDS             22298..24487
                     /gene="pdeA"
                     /locus_tag="ACHLCY_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0368975.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(24537..25739)
                     /gene="nupC"
                     /locus_tag="ACHLCY_001695"
     CDS             complement(24537..25739)
                     /gene="nupC"
                     /locus_tag="ACHLCY_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP0368976.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            26075..27313
                     /gene="mntH"
                     /locus_tag="ACHLCY_001696"
     CDS             26075..27313
                     /gene="mntH"
                     /locus_tag="ACHLCY_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP0368977.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQLLSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(27454..27780)
                     /gene="ypeC"
                     /locus_tag="ACHLCY_001697"
     CDS             complement(27454..27780)
                     /gene="ypeC"
                     /locus_tag="ACHLCY_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP0368978.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(27895..29151)
                     /gene="yfeO"
                     /locus_tag="ACHLCY_001698"
                     /pseudo
     CDS             complement(27895..29151)
                     /gene="yfeO"
                     /locus_tag="ACHLCY_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
     gene            29355..30320
                     /gene="glk"
                     /locus_tag="ACHLCY_001699"
     CDS             29355..30320
                     /gene="glk"
                     /locus_tag="ACHLCY_001699"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP0368979.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVYDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30539..30865
                     /gene="fryB"
                     /locus_tag="ACHLCY_001700"
     CDS             30539..30865
                     /gene="fryB"
                     /locus_tag="ACHLCY_001700"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP0368980.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            30887..32134
                     /gene="fryC"
                     /locus_tag="ACHLCY_001701"
     CDS             30887..32134
                     /gene="fryC"
                     /locus_tag="ACHLCY_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP0368981.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            32149..33234
                     /gene="ypdF"
                     /locus_tag="ACHLCY_001702"
     CDS             32149..33234
                     /gene="ypdF"
                     /locus_tag="ACHLCY_001702"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0368982.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            33234..34271
                     /gene="ypdE"
                     /locus_tag="ACHLCY_001703"
     CDS             33234..34271
                     /gene="ypdE"
                     /locus_tag="ACHLCY_001703"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0368983.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            34296..36791
                     /gene="ptsP"
                     /locus_tag="ACHLCY_001704"
     CDS             34296..36791
                     /gene="ptsP"
                     /locus_tag="ACHLCY_001704"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0368984.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILTRASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(36794..37651)
                     /gene="ypdC"
                     /locus_tag="ACHLCY_001705"
     CDS             complement(36794..37651)
                     /gene="ypdC"
                     /locus_tag="ACHLCY_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0368985.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(37664..38398)
                     /gene="ypdB"
                     /locus_tag="ACHLCY_001706"
                     /pseudo
     CDS             complement(37664..38398)
                     /gene="ypdB"
                     /locus_tag="ACHLCY_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
     gene            complement(38413..40110)
                     /gene="ypdA"
                     /locus_tag="ACHLCY_001707"
     CDS             complement(38413..40110)
                     /gene="ypdA"
                     /locus_tag="ACHLCY_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP0368986.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         9803 a        10251 c        10420 g         9757 t
ORIGIN      
        1 tcgtcgcttc cattttccgg agtaataatg aagcatgaat tatcaagtat gaaggcattt
       61 gtcatactgg cagagtccag ttcatttaac aatgctgcta aattactcaa tattacgcaa
      121 cctgcattaa cgcgaagaat aaaaaagatg gaggaagatt tacatattca actatttgag
      181 cgtacaactc gcaaggttac gttaacaaaa gcaggaaaaa ggttgctccc ggaggcgcgg
      241 gaattaataa agaaattcga tgaaacgctt tttaatattc gtgatatgaa tgcttatcat
      301 cgtggtatgg tgacgttagc gtgtattccg accgcagtat tttatttttt accgctggca
      361 atcggtaaat ttaatgagct gtatcccaat attaaagtgc ggattctgga acaaggcacg
      421 aataattgca tggaatcggt gctgtgtaac gaatcagact ttggcataaa catgaacaac
      481 gtcacaaatt catccattga ttttacccca ctggtcaacg agccattcgt gctggcctgt
      541 cggcgtgacc atccattggc caaaaagcag cttgtagaat ggcaggagtt agtgggttac
      601 aaaatgattg gcgtacgttc ctccagcggc aaccgattgt tgatagaaca acagctagcc
      661 gacaagccct ggaagctgga ctggttttac gaagtgcgtc atctttcgac gtcattagga
      721 ctggtagagg cgggactggg gatttcggcg cttcctggtc tggcaatgcc ccatgcgccc
      781 tattcttcga ttattggtat tccactggtg gaacccgtta tacgtcggac attggggatt
      841 attcgccgca aagatgccgt gctttctccg gcagcagaac gcttttttgc cttgctgatt
      901 aatttgtgga ctgacgataa agacaatttg tggaccaata tcgtcgaacg ccagagacat
      961 gcgttacagg agataggtta atacaccggc gcaggagaga ctgcgccggt taaatcagcg
     1021 gttagtgtgc gttaaccacc acccacattg gtccctgccc gaccgcataa cggccttttt
     1081 catgcagtag tccctgctcg ccaacaattt cgtataccga gatgtggtga gatttttgcc
     1141 cggcggcaat cagatacttg ccgctgtgat caacattgaa gccgcgcggc tgggtttccg
     1201 ttggctggaa gccttcttta ctcaacacgc tgccatcttc cgaaacgctg aaaacggtaa
     1261 tcaggctggc ggtacggtcg caggcgtata aatggcgacc atccggggtg atatgaatat
     1321 cagccgccca acgggtgtcg gagaagtttt ccggcatcat atccagcgtc tggacacatt
     1381 cgatattacc gtgcggatct ttcagttccc agacatccac tgagctgttt aactcattga
     1441 cgcaatacgc atattgttcg tttggatgga ataccatatg acgcgggccg gccccttcaa
     1501 cggtggtcac ttccgcaggg tcctgcgcca cgagatgacc atcatcgctg accgtaaaca
     1561 ggcaaatgcg atcctgcttt aatgccggaa cccacagcgt acggttgtcc ggtgagatat
     1621 tggcggaatg gcaaccgtcc agcccctcga ccacatcgac gacgcccact ggcaggccat
     1681 cttccagacg cgttacgctc acgttacccg cattgtaaga acctacaaag acaaactgcc
     1741 cctggtgatc ggtggaaata tgcgtcggac tacccggcag cgcagactct gcggcaaagg
     1801 tcagtgcgcc atcgtccggg gcgatactat acgccaggac gcgaaactca gggcgaacac
     1861 caacatagag ataacgtttg tccgggctga ccaccatcgg ctgcacctgc cccggcacat
     1921 cgacaacctg tgtcagcgtc agtgcgcctt catgattcag gttccagacg tgaatttgct
     1981 ggctctcagg gctggcgata taaactgttt gcttcatgaa tgctcctttg catttagcaa
     2041 ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta aaacgcagaa
     2101 ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt aaccttaatc
     2161 tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcacc ttattgaccc
     2221 cgaaaaagac cctgcttcct tcatcgatag aagccctggc ccgcgctcgc gaagcaggct
     2281 atcaattaat catcgtcaca ggtcaccatc acgtcgctat tcatcctttt tatcaggcgc
     2341 tggcgctgga tacacctgct atttgctgta atggcaccta tttgtatgat tatcatgcaa
     2401 aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc cctgcaactc attgagatgc
     2461 tgaatgaaca ccacattcac ggtctgatgt atgtggatga tgcaatggtc tatgagcacc
     2521 cgaccgggca tgtcattcgc acgtctaact gggcgcaaac cctgccgccg gaacagcgtc
     2581 cgactttcac acaagtcgct tctctggctg aaacggcgca acaagttaac gccgtatgga
     2641 agttcgccct gacgcacgat gacctgccgc aattgcagca ttttggtaag catgtcgaac
     2701 atgaactggg actggagtgt gaatggtcct ggcacgatca ggttgatatt gcacgcggcg
     2761 gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc gcaaggctgg tcgatggaaa
     2821 acgtcgtggc gttcggcgat aactttaatg atatcagtat gctggaggcc gctggtacag
     2881 gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg cgccaacatt gtgattggtg
     2941 ataacaccac cgacagcatt gcccagttca tttatagcca cctgatttaa tcaggcggtt
     3001 atcgacacac ttttaatttg cgcgtacagc cacaggccag gtttgatcac cagttcatcc
     3061 ctggcccacg ggctgatacg cgcccacagc gttttacctc cgacttccag ttccacttcc
     3121 acctggccgt tgtcgtcata actattaaca acttttgccc gcaatacgtt acgaatgctg
     3181 gtttgctgcg gcggttgtaa aaccagagaa acatcggaag cctgaatgcg gatacgtagc
     3241 gcagcttgca gcggttcgtc cagcttattg acccacaaat gctgatcgcc cagcgccagc
     3301 gcagtcatcg cgtaatgcgg atgatgctca agtaccgtca ctttcagaat gctactttgt
     3361 tgctctttcg gcagccacgg attcatcacg ctactacccc agacttcttc cagtgcgcca
     3421 aaggctttca cctgaccgtt ttccagtacc atcactctgt ctgccagatg gaggatctca
     3481 tccagcgaat ggctgacata caacatcggg atgttgattt cccgcgtcag acgttgcaga
     3541 taaggcaaca gttcgcgttt acgcggaata tccagtgacg ccagcggttc atccagcaac
     3601 agcaattctg gtgccgtcag caaagcccgc ccaatcgcta cgcgctgttt ttcgcctccg
     3661 gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc ctaaaagcgc caccagctta
     3721 tcgaactgat cgaccatgct tttcgccatg ccgtagcgca gattgccacg cactttgtaa
     3781 tgcggaaaca gccgcgcatc ctgaaaaaca tagccaacgc gacgcttttc tggcgtcagg
     3841 cagatacctt tttcggcatc atttagtacc cgaccattga ggacaatcct ccctttttgc
     3901 gggcgcgtca gtccactgat ggcgttaatc agcgaagttt ttccggcacc ggagacgcca
     3961 aagatagcag tgatgccatt ggcgggcagc gtttcattaa tggtcaggca atggttgccc
     4021 aaggtctggg aaaaattcag ttccagcatg attagcgccc cgcccgttca cggctgattc
     4081 tggccagcca ttctgaaatc aacagggaga tcatcgccag cgcaatagaa ataatgcaca
     4141 gtctcgccgc gccactttcc ccgccggggg tctggatcag ggtatacatg gcagaaggaa
     4201 tggttcgcgt ttcaccagga atgttcgaca caaaggtgat ggttgcacca aactcaccga
     4261 gagaacgagc aaaagccagt accgtaccaa caataattcc cggtaaggtc agcggtaacg
     4321 tgatagtaaa gaaaacgcgc cagcgcccgg cccccagtgt tcttgcggcc tgttcgagtt
     4381 tgacatcaac cccttccagc gccagacgaa ttgcccgcac catcagcgga aacgacatga
     4441 cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa ggtaatacca aaccagtcat
     4501 acagacgttc accgataaat ccgcgccgtc ccatcgaaac taataataag taaccgacga
     4561 ccacgggcgg taacaccagc ggtagatgca gtacgctgtc gagcagagct ttgcccggaa
     4621 acgtgcaacg caccagtaac caggcaaaaa agatcccaaa cggcaggcta aacagcacag
     4681 ccagggaaga aactttcagg cttaataaaa ctgcctgcca ttctggatcg gtcagtatca
     4741 ttacttggtt gtaaatccgt aacgtttaaa gatttccgca gcctgcgacc ctttcaaata
     4801 atcataaaat gctttcactg tcgcattgtt atgctcttcc acaacagcaa ccggatattc
     4861 cactttttta tgtgaatctt ccgggaaggt ggcaaccact tttacccctt tgctggcaac
     4921 tgcgtcagaa ccgtaaacaa tgcccaacgg cgcttcattg cgttcgacca acgccagcgc
     4981 gccacgaaca tcttccgccg gagccagttt cggggagagc gtatcccatg cgcccagttt
     5041 ttgcagtgct tctttcgcat aaatgccagc agggacatgt tccggatcgc caaccgcgag
     5101 gcgaccgcca ttcagcagcg aagtccagtt ggttttgctg tcgatggtga aatctttctg
     5161 ctcgctggct ttcggtgcta caacgaccag gctattgccg agcagtgtct ggcgcgaagc
     5221 cgtatcgatc gcttttttat caaccgcata atccatccat ttctgatcgg cagagataaa
     5281 cagatccgca ggcgctcccg cttcaatctg acgggccaga gtagatgacg aagcgaaaga
     5341 agaaaccaca tccacgcctt tttctttttt atactgcgta gcaatgtcct gcattgcgtt
     5401 agtcagtgat gcggcggcga acaccgtgat tttcccttca tctgccagtg tattgccagc
     5461 aacagcgaaa gagagtgccg ccccggcaaa caagttcaac catttacgag ccattggtaa
     5521 ccccttaatg taacgttatg taggcgacaa tataacggct agctcagaat tttcccagca
     5581 gttattgcta acctttgtga ggtagataag aaaaaatatc ggctggaaaa gcaggaagtt
     5641 gagaaaaaga aatgcccgac taagcgggca ttcagggaat caatgatttt gtccgggctg
     5701 gtctttttta ccaacaccag aaaagatgtt gaatacttca ccaagaccgt aaatcagacc
     5761 caggatgatg gccatcacga caggtaccat gattacggcg aataccagac tttttaataa
     5821 ctctaacatg gtcaactcca gatatagtca tgagactatt ctaaccgcta agcacagaaa
     5881 agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca caataagact
     5941 tttgccagga cattgttatg caggccgaaa tccttctcac ccttaagctc caacaaaaat
     6001 tattcgccga cccgcgccgg atttcgctac taaaacacat tgcgctttcc ggttccatta
     6061 gccagggagc gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc attaacgaga
     6121 tgaatcagtt aagtgagcat attctggtcg agcgcgcaac aggcggtaaa ggtggcggcg
     6181 gcgcagtact gacccgctat ggtcagcgac tgattcagct ctatgactta ctggcgcaaa
     6241 tccagcaaaa agcctttgat gtgttaagtg acgatgacgc cctgccgctg aacagcctgc
     6301 tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg taaccagtgg ttcggcacca
     6361 tcaccgcccg cgatcatgat gacgttcaac agcatgttga tgtcttactg gctgacggaa
     6421 aaacacgcct gaaagtcgca attaccgcac aaagcggcgc gcgtctgggg ctggatgaag
     6481 gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg tattactcag gacgaggcgg
     6541 tcgcacaaaa cgccgacaac caattaccgg gtattattag tcatattgag cgcggcgcag
     6601 agcagtgcga agtattaatg gcgctacccg acgggcaaac actgtgcgcc acagtgccgg
     6661 taaatgaagc gacttctctt cagcaagggc agaatgtcac ggcctacttt aatgccgaca
     6721 gcgtgattat cgccacgctg tgctaagcgt gttgacaatt tgttatgaaa cacgtatccc
     6781 tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat tttgcaaggc
     6841 acgtttcgtc ttagcgacac aaaaacgctg caattgcctc agctaacgtt aaacgcgggt
     6901 gatagttggg cgtttgtcgg ttcgaatgga agcgggaaat cggccctggc ccgcgcgctg
     6961 gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc agttttccca catcactcgt
     7021 ctctccttcg agcaattgca aaaactcgtc agcgacgaat ggcagcggaa taacaccgat
     7081 atgctcggcc ctggcgaaga tgacaccgga cgcactacgg ctgagatcat tcaggatgaa
     7141 gtaaaggatg caccgcgttg catgcaactg gcgcagcagt tcggtattac cgccctcctc
     7201 gaccgacgct ttaaatacct ttccactggc gagacgcgaa aaaccctgct gtgtcaggcg
     7261 ctgatgtcgg agcctgactt gttgattctt gatgagccgt tcgatggcct ggatgttgcc
     7321 tcacgtcagc agctggctga gcgactcgcc tcgttacatc agtccggtat tactctggta
     7381 ctggtgctca atcgcttcga tgagatcccg gagtttgtcc agtttgctgg cgtgctggcg
     7441 gattgcacgt tagcggaaac tggcgctaaa gaggaactgc tccaacaagc actcgtcgcg
     7501 caactggcgc atagtgaaca gcttgaaggt gtgcaactgc cggagccgga tgaaccttca
     7561 gcacgtcacg ccttacccgc caacgaaccg cgcattgtgc tgaacaatgg cgtggtttct
     7621 tataacgatc gccccattct taataacctt agctggcagg tgaatccagg tgaacactgg
     7681 caaattgtcg ggccaaatgg tgcaggaaaa tcgacgttat taagcctggt tactggcgat
     7741 catccgcaag gttacagcaa cgatttgacg cttttcggac gacgtcgcgg cagcggcgaa
     7801 accatctggg atatcaaaaa gcatatcggt tacgtcagca gtagtttgca tctggattac
     7861 cgggtcagca ctaccgtgcg taatgtgatt ctttctggct attttgattc gattggcatt
     7921 tatcaggccg tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga tattctcggc
     7981 attgataaac gtacggctga cgctccgttc catagtcttt cctggggaca gcagcgtctg
     8041 gcgctgatcg tccgcgcact ggtgaaacat ccgacgttgc ttattctcga tgaaccacta
     8101 caggggcttg atccgctcaa tcgccagctt atccgccgtt ttgttgatgt gctgattagc
     8161 gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg aagatgcgcc tgcctgtatt
     8221 acccatcgcc ttgagttcgt gccggacggt ggactctatc gctatgtgct gacaaaaata
     8281 tattgagtcg gtagtgctga ccttgccgga ggcggcctaa acgccctctc cggccaacgg
     8341 ttcgacgcat gcaggcatga aaccgcttct tttttcagat aaaaagcgca atcattcata
     8401 aaccctctgt tttataatca cttaatcgcg cataaaaaac ggctaaattc ttgtgtaaac
     8461 gatttcacta atttattcca tgtcacactt ttcgcatctt tgttatgcta tggttatttc
     8521 ataccataag cctaatggag cgaattatga gagttctggt taccggtggt agcggttaca
     8581 ttggaagtca tacctgtgtg caattactgc aaaacggtca tgatgtcatc attcttgata
     8641 acctctgtaa cagtaagcgc agcgtactgc ctgttatcga gcgtttaggc ggcaaacatc
     8701 caacgtttgt tgaaggcgat attcgtaacg aagcgttgat gaccgagata ctgcacgatc
     8761 acgctatcga caccgtgatc cacttcgccg ggctgaaagc cgtcggcgaa tcggtacaaa
     8821 aaccgctgga atattacgac aacaatgtca acggcactct gcgcctgatt agcgccatgc
     8881 gcgccgctaa cgtcaaaaac tttattttta gctcctccgc caccgtttat ggcgatcagc
     8941 ccaaaattcc atacgttgaa agcttcccga ccggcacacc acaaagccct tacggcaaaa
     9001 gcaagctgat ggtggaacag atcctcaccg atctgcaaaa agcccagccg gactggagca
     9061 ttgccctgct gcgctacttc aacccggttg gcgcgcatcc gtcgggcgat atgggcgaag
     9121 atccgcaagg cattccgaat aacctgatgc catacatcgc ccaggttgct gtaggccgtc
     9181 gcgactcgct ggcgattttt ggtaacgatt atccgaccga agatggtact ggcgtacgcg
     9241 attacatcca cgtaatggat ctggcggacg gtcacgtcgt ggcgatggaa aaactggcga
     9301 acaagccagg cgtacacatc tacaacctcg gcgctggcgt aggcaacagc gtgctggacg
     9361 tggttaatgc cttcagcaaa gcctgcggca aaccggttaa ttatcatttt gcaccgcgtc
     9421 gcgagggcga ccttccggcc tactgggcgg acgccagcaa agccgaccgt gaactgaact
     9481 ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac ctggcactgg cagtcacgcc
     9541 atccacaggg atatcccgat taaggaacga ccatgacgca atttaatccc gttgatcatc
     9601 cacatcgccg ctacaacccg ctcaccgggc aatggattct ggtttcaccg caccgcgcta
     9661 agcgcccctg gcagggggcg caggaaacgc cagccaaaca ggtgttacct gcgcacgatc
     9721 cagattgttt cctctgcgca ggtaatgtgc gggtgacagg cgataaaaac cccgattaca
     9781 ccaggactta cgttttcact aatgactttg cggccttgat gtctgacacg ccagatgcgc
     9841 cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg cggtaccagc cgggtgatct
     9901 gcttttcacc ggatcacagt aaaacgttgc cagaactgag cgttgcggca ttgacggaaa
     9961 tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa aacgtacccg tgggtgcagg
    10021 tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc gcatccgcac ggacagattt
    10081 gggcaaatag cttcctgcct aacgaagctg agcgcgaaga ccgcctgcaa aaagaatatt
    10141 ttgccgaaca gaaatcacca atgctggtgg attatgttca gcgcgagctg gcagacggta
    10201 gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt gccttactgg gctgcctggc
    10261 cgttcgaaac gctactgctg cccaaagccc acgttttgcg gatcaccgat ttgaccgacg
    10321 cccagcgcag cgatttggcg ctggcgttga aaaagctgac cagtcgttat gacaacctct
    10381 tccagtgctc cttcccctac tctatgggct ggcacggcgc accatttaat ggcgaagaga
    10441 atcaacactg gcagctgcac gcgcactttt atccgcctct gctgcgctcc gccaccgtac
    10501 gtaaatttat ggttggttat gaaatgctgg cagagaccca gcgagacctg accgcagaac
    10561 aggcagcaga gcgtttgcgc gcagtcagcg atatccattt tcgcgaatcc ggagtgtaag
    10621 aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa cgcatttggc taccctgcca
    10681 ctcacaccat tcaggcgcct ggccgcgtga atttgattgg tgaacacacc gactacaacg
    10741 acggtttcgt tctgccctgc gcgattgatt atcaaaccgt gatcagctgt gcgccacgcg
    10801 atgaccgtaa agttcgcgta atggcagccg attatgaaaa tcagctcgac gagttttccc
    10861 tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc gaactacgtt cgtggcgtgg
    10921 tgaaacatct gcaactgcgt aacaacagct tcggcggtgt ggacatggtg atcagcggca
    10981 atgtgccgca gggtgccggg ttaagttctt ccgcttcact ggaagtcgcg gtcggaaccg
    11041 tattgcagca gctttattat ctgccgctgg acggcgcaca aatcgcgctt aacggtcagg
    11101 aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat ggatcagcta atttccgcac
    11161 tcggcaagaa agatcatgcc ttgctgattg actgtcgctc actggggacc aaagcagttt
    11221 ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa cttcaaacgt accctggttg
    11281 gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg tgcgcgtttc ttccagcagc
    11341 cagccctgcg cgatgtcacc attgaagagt tcaacgctgt tgcacatgag ctggacccaa
    11401 tcgtggcgaa acgcgtgcgg catatcctga ctgaaaacgc ccgcaccgtt gaagctgcca
    11461 gcgcgctgga gcagggcgac ctgaaacgta tgggcgagtt gatggcggag tctcatgcct
    11521 ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga cactctggta gaaatcgtca
    11581 aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg cggcggattt ggcggctgta
    11641 tcgtcgcgtt gatcccggaa gagctggtgc ctgccgtaca gcaagctgtc gctgaacaat
    11701 atgaagcaaa aacaggtatt aaagagactt tttacgtttg taaaccatca caaggagcag
    11761 gacagtgctg aacgaaactc ccgcactggc acccgatggt cagccgtacc gactgttaac
    11821 tttgcgtaac aacgcaggga tggtagtcac gctgatggac tggggtgcga ctttactttc
    11881 agcccgtatt ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct gtgccagccc
    11941 ggaatgctat caggatcagg ccgcgtttct gggggcctct attggtcgtt atgccaaccg
    12001 catcgccaat agccgttata cctttgacgg tgaaaccgtg accctttcgc caagtcaggg
    12061 cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc agattgtgaa
    12121 ccagaacgat cgtcaggtgc tgtttgccct gagttcagat gatggcgatc agggcttccc
    12181 gggtaatctc ggcgcaacgg tgcaatatcg tctgaccgac gataaccgta tctccattac
    12241 ttatcgcgcc acagttgata aaccttgccc ggtgaatatg actaatcacg tctatttcaa
    12301 tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg cagattctgg cggacgaata
    12361 tcttccggtt gatgaaggcg gcattccgca cgacggcttg aaatctgtcg ccggaacgtc
    12421 ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt cttgccgacg acgatcagcg
    12481 caaagtgaaa ggttacgatc acgcattctt gttacaggcc aaaggcgatg gcaagaaagt
    12541 ggcggcgcat gtctggtcag cagatgaaaa attgcagctg aaggtctaca ccaccgctcc
    12601 ggctcttcaa ttctactccg gcaacttcct cggcggcaca ccgtcgcggg gaaccgaacc
    12661 ttacgccgac tggcaagggc tggcgttgga aagcgagttt ctgccggaca gcccgaacca
    12721 ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc gaagagtatt ccagcctgac
    12781 ggaatatcag tttattgctg agtaatgtta agccctcgtc atgagggctt tatcttcata
    12841 ttgttcaaat caccagcaaa caccgacata tttgcaactc aatattcaca acaaccttac
    12901 actgcgccac tattttcgct atggttatgc gtaagcattg ctgttgcttc gtcacggcaa
    12961 tataatgaga attattatca ttaaaagatg atttgaggag taagtatatg gctgtaacta
    13021 agctggttct ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt ttcaccggtt
    13081 ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc aaaagcagca ggtaagctgc
    13141 tgaaagagga aggttacagc tttgactttg cttacacttc tgtgctgaaa cgcgctatcc
    13201 ataccctgtg gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt gagaaatcct
    13261 ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct gaacaaagca gaaaccgctg
    13321 aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtc actccgccgg
    13381 aactgactaa agatgatgag cgttatccgg gtcacgatcc acgttacgcg aagctgagcg
    13441 agaaagaact gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg atcccttact
    13501 ggaatgaaac tattctaccg cgtatgaaga gcggtgagcg cgtgatcatc gctgcacacg
    13561 gtaactcttt acgtgcgctg gtgaaatatc ttgataacat gagtgaagaa gagattcttg
    13621 agctgaatat cccgaccggc gtgccgctgg tgtatgagtt cgacgagaat ttcaaaccgc
    13681 tgaaacgcta ttatctgggt aatgctgacg agatcgcagc gaaagcagcg gcggttgcta
    13741 accagggtaa agcgaagtaa acgtcattcg tttaaaaatg agaaagccga ctgcaagtga
    13801 gccggctttt ttgttgctaa caatggagca cattgcctga tgcgacgctg cgcgtcttat
    13861 caggcctgtg gtgattcatc ggatacgcca ctctgacggc gcatccgaca attaaacctt
    13921 acccgcgacg cgcttttact gcattcgcca gttgacgtaa cagagcatcg gtatcttccc
    13981 agccgatgca ggcatcggtg atgctcttac cgtaggccag cggctccccg ctctcgaggc
    14041 tctgattgcc ttccaccaga tggctttcca ccatcacgcc aataatggct gtttcgccac
    14101 cggcaatctg ctggcaaacg tcagcacata catccatctg ctttttgaat tgtttggacg
    14161 agttagcatg gctgaaatcg atcatcacct gtgctggcag gcctgctttg ttcagccctt
    14221 ctttcacttc agcaacgtgc ttcgcgctgt agttaggctc tttaccgccg cgcagaatga
    14281 tatggcaatc accgttaccg ctggtattca caatcgccga atgcccccat ttagttacgg
    14341 acaggaagca gtgcggcgca ccggcggcat taatggcatc gatagccact ttaatcgtac
    14401 cgtctgtgcc atttttaaaa cctaccggac aagaaagacc agacgccagt tcgcggtgca
    14461 cctgcgattc ggtggtacgt gcaccaattg cgccccagct catcaggtca gcgagatatt
    14521 gtggggtgat catatcgaga aactcacccg ccgctggcag accgctgtcg ttaatatcaa
    14581 gcagcaattt acgggctata cgcagaccgt cgttgatctg gaagctgtta tccatatgcg
    14641 gatcgttaat cagccctttc cagcccaccg tggtacgcgg cttttcaaaa tagacgcgca
    14701 ttacgatttc cagctcatct ttcagctctt cacgcagcgc cagcaagcga gtggcatact
    14761 cttttgctgc gacaggatca tgaattgagc atgggccaat caccaccaac aggcgatcat
    14821 cattaccttt caggatctta tggatcgctt ttcgggcatg ggcaaccgta ttcgcggcat
    14881 tttcagtagc ggggaatttt tccagcaatg cgacaggagg aagtaactct ttgatttctt
    14941 tgatgcgtaa atcgtcgttc tgataattca tgtctgttcc agtgttgcca tacttatctt
    15001 agtgaatgca atacttccaa tctatatctt ccgtcagaat gtgtaaacgg ggttttacac
    15061 tatgaacgga ttaatcctgg aattagcaag aaaaacgcca gattgtcgcg aaaaacgaga
    15121 tctctcctac aatttctaac tgtaactcct ttgtttgtta attatttcaa gattctctgc
    15181 tgcgtttcat aacctggctg aaatcttaaa ccaatgcctt atattcacct gcaaatgcac
    15241 tgttggaaga ggttatccga cataacgacc ataacaggag catcctatga aaatgacaaa
    15301 actggccaca ttatttctga ctgccactct aagccttgcc agcggtgccg cactggctgc
    15361 cgatagcgga gcgcaaacta ataacggcca ggcaaacgcc gcagctgatg cgggccaggt
    15421 agcccctgac gcccgtgaaa atgtcgcgcc aaataacgtc gacaataacg gggtaaatac
    15481 cggttctggt ggcacaatgc tgcattcgga tggttcttca atgaacaatg acggaatgac
    15541 caaagatgaa gagcacaaga acaccatgtg caaagatggt cgctgtccgg atatcaataa
    15601 aaaggtacaa accggtgatg gcatcaataa tgatgtcgat accaaaaccg acggtaccac
    15661 gcagtaaccc tgcaaaatag ccaataatgg gagagcggag tctctccctt ttaatttttc
    15721 acttctaaaa gcagtatgat gtcataaagt taagtgagaa taattaaggg atgactcgtc
    15781 atggcgcact cacactcaca cacgtcctca cacctgccag aagataataa tgctcgtcgc
    15841 ttgttgtatg ctttcggcgt gactgccggg tttatgctgg tagaagtcgt tggtggtttt
    15901 ctttctggtt ctctggcatt gctggccgat gcgggtcata tgttgaccga tactgccgcc
    15961 ctgcttttcg ccctactcgc cgtgcaattt tcacgtcgtc ctcccaccat tcgccacact
    16021 ttcggttggc taagactgac cacgctcgcg gcgtttgtga atgccatagc cctcgtggta
    16081 attaccattt tgattgtctg ggaggcgata gaacgcttcc gcacgccgcg tccggtcgag
    16141 ggcggcatga tgatggcaat tgccgtggct gggttgctgg caaatatact ttctttctgg
    16201 ttacttcatc acggcagcga agagaaaaac ctcaacgtac gagcagcggc actgcatgtg
    16261 ctcggggacc tgctgggttc ggttggggct attattgccg cgttgattat tatctggact
    16321 ggctggacac ctgctgaccc cattctctcg atactggtgt cgcttctggt tctgcgtagc
    16381 gcgtggcgac tgttgaaaga tagcgtgaat gaattacttg aaggtgcacc ggtatcgctg
    16441 gatatcgctg aactgaagcg tcgtatgtgc cgggaaatcc cggaagtccg caatgtgcac
    16501 catgtacatg tatggatggt aggcgagaag ccggtgatga cgttgcatgt gcaggtgatc
    16561 ccgccacacg atcacgatgc cttgttggat cagatccaac actacctgat ggatcactat
    16621 cagattgagc acgccaccat tcagatggaa tatcaacctt gtcatgggcc agactgccac
    16681 ctgaataaag gggtgtctgg ccattcacat catcaccatt aatgggacag cgcgcgtgag
    16741 cctctttcac gtgcgctgtt gatccacatc cggctgccgt tgagcgcaat aaaggtcagg
    16801 atgatgtact ccagagacat ggcgtaaacg ccctgaagtg caaaaataac gacgctaatc
    16861 acgttaataa tcacccacaa cagccagttt tccacatact tacgcgtcat cagaatcatt
    16921 gccacgatag ataacaccat catgcatgaa tcccagaacg ggaaagcgtc cggttgcagt
    16981 tcaggcatca caacctgtaa tcctaatgct tgcatgatca tgactgccag gcgggtcaaa
    17041 aatgcaaaca ccggattgat aaataccgtc atcagaccaa tcgaaacaac gcaaaccgcc
    17101 aaccagctga gtgccttcgg caatggcaac cagcgaattt tcaactccgc ctcgttctga
    17161 ctggtttgtc gcgaccacgc ataccaaccg taaatattcg cggcaaagaa aaacacctgt
    17221 aatagcaggc tggcatacag ctgaatctga aagaaaataa tgccaaacaa ggtgacgttg
    17281 atcaggccaa agaagtagtt gctgatcttc tccagactgg caaggccaat acacagcaac
    17341 ccggcgatcg tgcctaccgc ttcgatccat gagagatcat aaccgcctgc ccctattggt
    17401 atatgtacca ggatattctg cacactaaaa aaatccatct ttcccccaaa gcgtaacgtt
    17461 acgccttatt attcgttatc cacgtagtgt agccgcaaaa tccagcatac gattgagcgg
    17521 caccagcgcc ctctctcgca gcctttcatc aacatgaacc tcgtgattgc ttccttcctg
    17581 ttctaatgcc tctgcgatgg actgaaggcc attcatggcc atccacggac aatgcgcgca
    17641 gctgcggcag gttgcaccct cacctgcggt tggtgcttcc agtaactctt tatctggcac
    17701 tgcctgctgc attttgtaga aaatgccccg atcggttgcc acgataagcc gctgatgtgg
    17761 caatgttttc gcagcagcga tcagttgact ggtggaaccg accgcatccg ccatatcgac
    17821 aatagcttgt ggtgattctg gatgcaccag tatggcagca tccgggtatt cttcttgcaa
    17881 gcgggttaac gcctgagtct taaattcatc atgcacaata caggcaccct gccagcatag
    17941 aatgtctcca cccgtctgtt tttgcacgta acgccccaga tgtttgtcgg gtgcccagat
    18001 gattttttca cccaaactat caagatgatc aataagttcg acggcaatgc ttgaagttac
    18061 cacccaatct gcgcgcgctt ttaccgcagc agaagtgttg gcgtagacga cgacagtacg
    18121 atcgggatgg gcatcgcaaa atgcgttaaa ttcttcaaca gggcagccga gatccagtga
    18181 acattcagcc tgaagtgtcg gcatcagaat tgttttttcc ggactgagaa ttttggcggt
    18241 ttctcccata aatctcaccc cagcgactaa caaagtagaa gcgggatgct ttgcaccgaa
    18301 gcgcgccatt tccagagaat cagaaataca gccaccggtt tcttctgcca gttgttgaat
    18361 ttcgggatcg gtatagtagt gggcaaccat caccgcatta cgttctttta gcagacgttt
    18421 tatcttctcg cggtaatacg ctttttcatc aatgcttaac ggcgtcggct tcggggggaa
    18481 aggataaatc gccgtgtctg gatcaaacat tacgctcatc ttaccatctc gttttacagg
    18541 cttaacgtta aaaccgacat tagcgtaaca ttcgctgtta tcggtgtaat ttgttttata
    18601 tgctaaacaa gatagtcgaa tggtggggaa aagtcactcg aattacgcct aattccaccg
    18661 acgtagagtt gcttgcacag taagctctga cgatgtcatc aggtttacaa agtgaaattg
    18721 aggatgcctg aatagcaaaa aagcgccttt agggcgcttt tttacattgg tgggtcgtgc
    18781 aggatgactc gcttcgctcg cccttcgggc cgtcgccgcg agcggctccg ttgtttcgct
    18841 gcgctcaact cgaacctgcg gcagcttcga atctttgagt atcgcagata gcaaaaaagc
    18901 gcctttaggg cgcttttttt acattggtgg gtcgtgcagg attcgaacct gcgaccaatt
    18961 gattaaaagt caactgctct accaactgag ctaacgaccc gaagtggtgg gtgatgacgg
    19021 gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc
    19081 acttctcaat ttcttgctac acggcggaga ctacataaag tagttggtgg gtgatgacgg
    19141 gatcgaaccg ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc
    19201 ccgctgtgtg gagtcgcatt atagggagag ttcaaaatga gtcaacgcat tttctaaaga
    19261 aattgttcgt tcgtcgtaaa tttaagcaag atgatcgcaa aacagaccgt gttgcgcaat
    19321 ttgtcaacga aaacaataat gcgtaaggtt gcaacccgaa ctacattgag gaatcaggcg
    19381 ggagtgatag aatatcgccc actcaatttt tccaggattt gccggttgtc ggcatctttc
    19441 taaacgtaag gccatttcat gaaaatcaaa actcgcttcg cgccaagccc aacaggctat
    19501 ctgcacgttg gcggcgcgcg tactgctctt tactcctggc tttttgcacg taaccacggc
    19561 ggtgagttcg tgctgcgtat tgaagacacc gatcttgagc gttccacgcc ggaagctatc
    19621 gaagccatta tggatggcat gaactggctg agcctggagt gggatgaagg tccgtactac
    19681 cagaccaaac gttttgatcg ctacaatgcg gtgatcgatc agatgctgga agagggcact
    19741 gcttataaat gctattgctc taaagagcgc ctggaagcgc tgcgcgaaga gcaaatggcg
    19801 aaaggtgaga agccgcgtta tgacggtcgc tgccgccaca gccatgagca tcatgctgat
    19861 gatgaaccgt gtgtcgtgcg ttttgctaat ccgcaggaag gttctgttgt ttttgacgat
    19921 cagatccgtg gtccgatcga gttcagcaac caggagctgg atgatctgat tatccgccgt
    19981 accgatggtt ccccaaccta taacttctgt gtggttgtcg atgactggga tatggaaatc
    20041 acccacgtta tccgtggcga agaccatatc aacaacacgc cgcgccagat caacattctt
    20101 aaggccctga aagcgccggt gccggtttac gcgcacgttt ctatgatcaa tggcgatgac
    20161 ggtaaaaaac tgtccaaacg tcacggggca gtcagcgtaa tgcagtatcg tgatgacggt
    20221 tatttgccag aagcactgct gaactatctg gtgcgtctgg gctggtccca cggcgatcag
    20281 gaaatcttca ctcgtgaaga gatgatcaaa tacttcactt tgaatgccgt cagcaaatct
    20341 gccagtgcgt tcaacaccga caagctgctg tggctgaacc atcactacat taacgcgctg
    20401 ccgccggagt atgttgctac tcacttacag tggcacattg agcaggaaaa tatcgatacc
    20461 cgtaacggcc cgcagctggc tgatctggtg aaactgctgg gcgaacgctg caagacgctg
    20521 aaagagatgg cacagagctg ccgttatttc tacgaagatt ttgctgagtt cgatgccgat
    20581 gcggcgaaaa aacatctgcg tccggtagcg cgtcagccgc tggaagtggt tcgtgacaaa
    20641 ctggccgcga ttactgactg gaccgctgaa aacgttcatc acgctattca ggcgacggcg
    20701 gatgagctgg aagtgggtat gggtaaagtt ggtatgccgc tgcgtgtcgc cgtaaccggt
    20761 gcggggcagt ctccagcact ggatgttacc gttcacgcaa ttggtaagac ccgcagtatc
    20821 gagcgtatca acaaagcgct ggattttatt gctgaacgcg aaaatcagca gtaattagcg
    20881 tgtaaaaaga taaacggcag gagataatat cctgccgttt tttatttatg ctgattcgtc
    20941 aatatcgaga cgggcgagga aattgcgaat tccttcacgt gacaaaaatt gcgcgacttt
    21001 ttcctgctca atgtctgtca tattatctat cgcgttgatg atttgtcgat acgccggggc
    21061 gcgtgctcca ggagcgtatt cagcaattct ctcttcagct tccatcaaca ttggtgtgtt
    21121 atggaaggct ggcgtgttct gaataaactc gcaaacttgc gtatcgacca gaataagtct
    21181 ggcacgaccg cctttcacgc cgggaaattt ttccgttttc cagccttttt ctctgatcca
    21241 acggttaaca gtttgtttag ccacaccgag gcattcagcc agttcttcgg tggtcatttt
    21301 gttgcgtaat cttttcatat tatttgctat cgcgaatccc taagcgttgt aacaatccga
    21361 taatcccttc ccgcagaagc aaggatgtaa actgtttttg ttcaactggc gtcatctctt
    21421 tcgccagtgt caccagcaat acttcaagcg gcgcatcacc agaaagggca ggcgcttctc
    21481 cctgaccttc tggacgttca gcattgcgaa tatattcacg aacctgttca ttgacgtgaa
    21541 ccagtcgggc tttgccaccc tggacgccag gttttggtga cgttgtccag ccttccttgc
    21601 gtacccattt attaatggtc tggcggctat agccggtcag tctggcaagt tcatctggtg
    21661 tcatcctctc cttgaacata acaattccct gaatgtatgt gggtctatta gtagttcatt
    21721 ttataacacc actttacagg aaaccgtgac atgaataact actgaatgcg agcggttttc
    21781 caaaattgtt catttgcctg atttttcagc gattgaaact acacgttgca atttgccgtt
    21841 gacacattcg ggcggaattc atatgatgcc gcccgtcaac tcgacaagct ttacgtgacg
    21901 gggctatagc tcagctggga gagcgcttgc atggcatgca agaggtcagc ggttcgatcc
    21961 cgcttagctc caccaaattt tgcacccagc aaatttggta cgtaaacgca tcgtggggct
    22021 atagctcagc tgggagagcg cttgcatggc atgcaagagg tcagcggttc gatcccgctt
    22081 agctccacca aatttccaac cctcgctgca aagcgggggt tttttgtctc tgctttttgc
    22141 cgcttttgta atacagtcta cgtccgggtt agcgccgcct ggtgaaagca tcattggatg
    22201 aaaaatcggc aacaggctgg cccctgtttg cttcgcgatg cgaataaact tattatttgt
    22261 gtgcctgaaa accccgatca gtgagagtag tgtactcatg tttgtggagc ataacctgat
    22321 aaaaaatatc aagatattca cactagcgtt tacgctcacc gtggtactta ttcagctatc
    22381 ccgttttatt tcgccacttg ccattatcca ttccagttat atctttctgg cgtggatgcc
    22441 actgtgcgta atgctgtcaa tcttgtttat ctttggctgg cgcggtgtcg ttcccgtttt
    22501 atgcgggatg ttttgcacca atctgtggaa ctttcatctc tcttttttac agactgcggt
    22561 catgctcggt agccagacgt ttgtcgtgtt gtgtgcctgc gcaatattac gctggcagct
    22621 ggggacgcgt tggcgttatg gattgaccag ccgatatgtc tggcaacgtc tgttctggct
    22681 tggtttggtg acgccgatcg gtatcaaatg cagcatgtat cttgtgggga gcttctttga
    22741 tttcccgcta aagatatcca cctttttcgg cgatgcggat gccattttca cggtcgttga
    22801 tttgctaagc cttttcaccg ctgtgctgat ttacaacatg cttttctact atctcactcg
    22861 catgattgta agtccccact ttgcgcagat attgtggcgc agggatatcg ctccgtcgtt
    22921 gggcaaagag aaacgcgcat ttaccttaag ctggctggca gcgttaagcg tgctgctact
    22981 tctgttgtgc acaccttatg aaaacgactt tattgccggt tacttggtac ccgttttctt
    23041 catcatcttt accctcgggg tcggtaagct tcgctatccg tttttaaatc tcacctgggc
    23101 tgtttcaacg ctttgcctgc tgaattacaa ccagaacttt ttgcaagggg tggaaaccga
    23161 atattcgctg gcatttattc ttgcggtgct gatttccttt agcgtttgcc tgctctatat
    23221 ggtgcgcatt tatcatcgca gtgaatggct taatcgccgc tggcatttgc aggcgctgac
    23281 agatccgtta acgctcctac ccaactttcg tgcgttggaa caagcgccgg agcaagaggc
    23341 gggcaagagt ttttgctgcc tgcgcattga taatcttgag tttatgagtc gtcattacgg
    23401 cttaatgatg cgcgttcact gtatccgctc aatttgccgt acgctgctgc cgttgatgca
    23461 ggaaaacgaa aagttgtatc aattgccggg tagtgaactg ctgttagtgc tgagcgggcc
    23521 ggaaacggaa gggcgactcc agcatatggt taacatcctg aatagtcggc aaattcactg
    23581 gaacaatacc gggctggata tgggctatgg tgctgcctgg gggcgttttg atggaaatca
    23641 ggaaaccctg caacccttgt tggggcagtt aagctggctg gcggagcaat cctgcgcaca
    23701 tcatcatgtg ctggcgctgg atagcagaga ggagatggtt tccgggcaga ccactaaaca
    23761 ggtgctattg ctgaatacca ttcgcacggc gttagatcag ggtgatttgc tgctctacgc
    23821 ccagccaatt cgcaacaaag agggtgaagg ttatgatgag atcctcgcgc gactgaaata
    23881 tgacggcggc attatgaccc cggataagtt tctgcccctt attgctcagt ttaaccttag
    23941 cgcgcgtttt gatttgcaag tgctggaatc cttgttgaag tggctggcaa cacacccttg
    24001 cgacaaaaaa ggaccgcgct tttcagtcaa tttaatgccg ctcacgctgc tgcaaaagaa
    24061 tattgccggg cggattattc gtctgtttaa gcgttatcac atctccccgc aggcggtcat
    24121 tcttgagatc accgaggagc aggcgttttc taacgcagaa agcagcatgt acaacatcga
    24181 gcagctgcat aagtttggtt tccggattgc gattgatgac tttggcactg gatatgccaa
    24241 ctacgaacgg ttaaagcgtt tgcaggctga tatcatcaaa attgatggcg tctttgtgaa
    24301 agatattgtc acgaatacgc tggatgcgat gattgtgaga tcaattaccg atctggcgaa
    24361 agcgaagtca ttgagtgtgg tcgcggagtt tgtcgagacg caacagcagc aggcgctatt
    24421 gcataagctc ggggtgcaat atctgcaagg gtatttgatt ggtcgcccgc agccattagc
    24481 tgattaacag gcgtaaaaaa accggggaat tatccccggc ttttttatgt atggtcttac
    24541 agcaccagtg ctgcgattga cgcagacagc acactcacca gggtagagcc gtaaaccagc
    24601 ttcagaccga agcgagaaac cacgttacct tgctcttcat tcaggccttt aactgcacct
    24661 gcgataatcc cgattgaaga gaagttagcg aaggaaacca ggaacacaga gatgatgcct
    24721 tcagcacgcg gagagagcgt ggaagcaatt ttctgcagat ccatcatcgc aacgaactcg
    24781 ttggaaacca gtttggtcgc catgatactg cccacttgca gtgcttcact ggaaggaaca
    24841 cccatcaccc atgcaatcgg atagaagatg tagcccagga tgccctggaa ggagatgctg
    24901 tagccaaacc agccagtaac ggtggcaaac agtgcgttca gcgcggcgat cagggcgata
    24961 aagccgatca gcatcgcggc aacgataatg gcaactttga aacctgccag aatgtattca
    25021 cccagcattt cgaagaagct ctgaccttcg tgcaggttgg acatctggat gttttcttca
    25081 ctggcatcaa cacggtaagg attgatcagc gacagcacga taaaggtgct gaacatgttc
    25141 agtaccagcg cagcaacgac gtatttcggt tccagcatgg tcatgtatgc accaacgatg
    25201 gacatcgaca cggtggacat tgccgtggca gccatggtgt acatacgatt acgggagatt
    25261 ttgccgagga tatctttata ggcaataaag ttttcagact gacccagaat cagggagctg
    25321 acggcgttaa aggattccag tttgcccatg ccgttgactt tggagagcag gaaaccaatt
    25381 gcgcggatga tcaccggcaa cacgcgaatg tgctggagaa taccgatcag tgcagagata
    25441 aagacgattg ggcacagcac tttcaggaag aagaatgcca ggccttgatc attcatgcta
    25501 ccaaagacga agttagtccc ttcgttggca aatccgagca gtttttcgaa catttcggag
    25561 aagcctttca cgaagcctaa accaacgtcg gagttcagga agaaccacgc cagtaacact
    25621 tcgataacaa gcagttgaat aacataacgg atacgaattt ttttgcggtc gctgcttacc
    25681 agcagtgcga gaatcgcaac aacggcaagt gccagtacaa aatgaaggac gcggtccata
    25741 tttgctccaa atatgaggca ggttaaattt ccgtgcacat tctatgtaac atggttaagg
    25801 aaaacgagat ctgacacaca ctataatgac gtcctgtgac aagcttcaaa aatagtgatc
    25861 tgtcatacat ttctgctatg ttgtgtatgg aagctgaaag ttatgtaaat gtgctaacat
    25921 taaacataac cataacattc aagtcatcaa tcttttcgta gtcataatcc tggtctatca
    25981 aagaaatcac cacaacccat tcaaatgaat ttgataatca ttctcgtttg gcatagcatg
    26041 aaacatagca aaggctatgt tttagaggca caagatgacg aactatcgcg ttgagagtag
    26101 cagcggacgg gcggcgcgca agatgaggct cgcattaatg ggacctgcgt tcattgcggc
    26161 gattggttat atcgatcccg gtaactttgc gaccaatatt caggcgggtg ctagcttcgg
    26221 ctatcagcta ctgtgggttg tcgtttgggc caacctgatg gcgatgctga ttcagctcct
    26281 ctctgccaaa ctagggattg ccaccggtaa aaatctggcg gagcagattc gtgatcacta
    26341 tccgcgtccc gtagtgtggt tctattgggt tcaggcagaa attattgcga tggcaaccga
    26401 cctggcggaa tttattggtg cggcgatcgg ttttaaactc attcttggtg tttcgttgtt
    26461 gcagggcgcg gtgctgacgg ggatcgcgac tttcctgatt ttaatgctgc aacgtcgcgg
    26521 gcaaaaaccg ctggagaaag tgattggcgg gttactgttg tttgttgccg cggcttacat
    26581 tgtcgagttg attttctccc agcctaacct ggcgcagctg ggtaaaggaa tggtgatccc
    26641 gagtttacct acttcggagg cggtcttcct ggcagcaggc gtgttagggg cgacgattat
    26701 gccgcatgtg atttatttgc actcttcgct cactcagcat ttacatggcg gttcgcgtca
    26761 acaacgttat tccgccacca aatgggatgt ggctatcgcc atgacgattg ccggttttgt
    26821 caatctggcg atgatggcta cagctgcggc ggcgttccac ttttctggtc atactggtgt
    26881 tgccgatctt gatgaggctt atctgacgct gcaaccgttg ttaagccatg ctgcggcaac
    26941 ggtctttgga ttaagcctgg ttgctgccgg actgtcctca acggtggtgg ggacactggc
    27001 agggcaggtg gtgatgcagg ggttcattcg cttccatatc ccgctgtggg tgcgtcgtac
    27061 agtcaccatg ctgccgtcat ttattgtcat tctgatggga ttagatccga cacggattct
    27121 ggttatgagt caggtgctgt taagttttgg tatcgccctg gcgctggttc cactgctgat
    27181 tttcaccagt gacagcaagt tgatgggcga tctggtgaac agcaaacgcg taaaacagac
    27241 aggctgggtg attgtagtgc tggtcgtggc gctaaatatc tggttgttgg tggggacggc
    27301 gctgggattg tagttgaatg agcgtcgcat ctggcactat tggcggatgc ggcgtaaacg
    27361 ccttatccgc cctacgcgtt aaagagccgg tttgtaggcc tgataagacg cgctagcgtc
    27421 gcatcaggca ttgtgctcca ttggaggacg cgtttaatgg cggtgcccat gtccgcggcc
    27481 ttttccgcga tgatcgtcac gatcgcgcca gccttcacga tagccacgct catatgcttt
    27541 acgcttatcc cagccacgat gatagccatt atcatgacgc caccagcggt ttttgcgcca
    27601 ttcataatta cgatgccagt aatcacgatc tcgccagtga ccaccgtccc agtaattacc
    27661 gtaatgatcg cgatcgccaa tttgtaattt gatcgatggc agtagggtga tttcacctgc
    27721 atttgctgca agcggggtaa atgccatcag ggcggccgcc agaaacagtg acctgaacat
    27781 tgttattctc cttcacgatc gaagccgtca gcggcctgtt aacgcaatat tacggggagg
    27841 taaagccccg tatcatcgcc ataactctta aatcataaag aagagcattt tttgctaaac
    27901 attatcgtgg ggtggctgtt gcttcggacg attgaccatc atcatcggct tacctgctaa
    27961 taacagccat gccggaagca tgacgataca gagcagcggt agcaatgtgg tattgggtac
    28021 aacgactgcc gccataaaaa gacttaacca gccatcgcgt gttaccacca gcacgatgcc
    28081 gagaatagcg caggaaacgg ttattgccgc tggaacggcg ggaacgtgct catgcagcat
    28141 caaccctaat gccacgccga caaacactgc cgggaagatt cgcccaccgc gaaagccact
    28201 ggcggcagca acgaccagag cggcaagttt aattaccgcc agcaaaaagt aatcgctggt
    28261 gctgaaagcc tgatttgcca ccatctgctg catctcatcc agccctttaa acagcgaaac
    28321 cggcccacca ataactccca gaataccgag aataaatccg ccaataccta gcacgagcac
    28381 cggatttttc atttgatgca tcatcgcgtg caaccgtggt aagcaccata cggcgaccat
    28441 tcctgctgcg atggcaatcg ccgcgacaat tgcaccgctg agaatatcgg tcatttccat
    28501 ctgttcgtaa tgcgcaatgg gcagtgaaaa atggggatgg aaaaataatc cggtagtaag
    28561 tgcaccagct gctgccgcca ttaatggcgc aaaaagacga tcccatagcg gaacttcact
    28621 actgccattt aaggtttgcg aaaatatcaa cgccgccgca acaggtgtac caaacagcgc
    28681 gccgatggtt ccggcagagg ctaaaatagt ccactccatt cggttgacgc gcggtaacag
    28741 acgagcgcca atggcaaccg caagggcgat attgacggtc atgatcggat gttccggccc
    28801 caggctgacg ccgccagcaa gaccgagaat taatgctacg ataagtcctg gtagcgcaga
    28861 gggcggaact ggtgcgccga tcagcggttc acaggcgggg tctggtccgg catgaccctg
    28921 gctgaaacgg ataaccaacc ccaccgcaat acccgttagc gttaatacac cgatgatcta
    28981 gagaggtgaa tcctgggcta tccccagagt tcccggcagt cgttgccaga gcaaattctg
    29041 taataccgag gcgattttca tcaccacaat cagaataaga ctggacgcaa tcccaattgc
    29101 cacggcgggg agcgataata acaacatggt tctggctcgc ggatggagca tgttctattc
    29161 cttatgcggg gtcagatact tagtttgccc agcttgcaaa aaggcatcgc tgcaattggt
    29221 gctgaaacga taaagtaaat gtgtgaccca gatcgatatt tacagggagc ctgcctttcc
    29281 ggcgttgttg ttatgccccc aggtatttac agtgtgagaa agaattattt tgactttagc
    29341 ggagcagttg aagaatgaca aagtatgcat tagtcggtga tgtgggcggc accaacgcac
    29401 gtcttgctct gtgtgatatt gccagtggtg aaatctcgca ggctaagacc tattcagggc
    29461 ttgattaccc cagcctcgaa gcggtcattc gcgtttatct tgaagaacat aaggtcgagg
    29521 tgaaagacgg ctgtattgcc atcgcttgcc caattaccgg tgactgggtg gcaatgacca
    29581 accatacctg ggcgttctca attgccgaaa tgaaaaagaa tctcggtttt agccatctgg
    29641 aaattattaa cgattttacc gctgtatcga tggcgatccc gatgctgaaa aaagagcatc
    29701 tgattcagtt tggtggcgca gaaccggtcg aaggtaaacc tattgcggtt tacggtgccg
    29761 gaacagggct tggggttgcg catctggtcc atgtcgataa gcgttgggta agcttgccgg
    29821 gcgaaggcgg tcacgttgat tttgcgccga atagtgaaga agaggccatt atcctcgaaa
    29881 tactgcgtgc ggaaattggc catgtttcgg cggaacgcgt gctttctggc cctgggctgg
    29941 tgaatttgta tcgcgcaatt gtgaaagctg acaaccgcct gccagaaaat ctcaagccaa
    30001 aagatattac cgaacgtgcg ctggctgaca gctgcaccga ttgccgccgc gcgttgtcgc
    30061 tgttttgcgt cattatgggc cgttttggcg gcaatctggc gctcaatctc gggacatttg
    30121 gcggcgtgtt tattgcgggc ggtatcgtgc cgcgcttcct tgagttcttc aaagcctctg
    30181 gtttccgtgc cgcatttgaa gataaagggc gctttaaaga atatgtctat gatattccgg
    30241 tgtatctcat cgtccatgac aatccgggtc ttctcggttc cggtgcacat ttacgccaga
    30301 ccttaggtca cattctgtaa atccttcctg ttatatcggg aggtaactct cccgataatc
    30361 ttttaaatca tacagttaat tcaatttttc tttgtgtccc ctcacaagac cgacctgcgt
    30421 cacacttccg tacagcggga ttaattctcc agtaaatgca ttatttgtct ggtaacggcg
    30481 atttgttttg cacgttcata atttcactcg tcaactatca ctgaatgagg aattaacgat
    30541 gagtaagaaa ctaattgcct tatgtgcctg cccaatgggc ctggctcaca cttttatggc
    30601 cgctcaggcg ctggaagaag cggcggtaga agccggttat gaagtgaaaa ttgaaaccca
    30661 gggcgcggac ggtatccaga atcgcctgac ggcgcaggat atcgccgaag caaccatcat
    30721 catccactcc gtggcagtta ccccggaaga taatgagcgt ttcgaatcac gcgacgttta
    30781 tgaaatcact ttgcaggacg caattaaaaa cgctgcgggc atcatcaaag aaatcgaaga
    30841 gatgattgcc tctgaacaac agtaatattc gacagggata attgttatgg ccattaaaaa
    30901 acgcagtgca accgttgtgc ctggcgcatc cggtgcggca gcggcagtta agaatccgca
    30961 ggcctctaaa agcagcttct ggggtgaact cccgcagcat gtgatgtcag ggatttcacg
    31021 catggtgccg accttaatta tgggcggtgt gatcctcgct ttcagccagc tgattgctta
    31081 tagctggctt aaaattcctg ctgagatcgg catcatggat gctcttaata gcgggaaatt
    31141 ctccggtttc gacctctcct tactgaaatt tgcctggctg tcgcagtcct tcggcggcgt
    31201 gttgtttggt tttgccattc cgatgtttgc tgcttttgtg gcgaactcta tcggtggcaa
    31261 actggcgttc ccggctggtt ttatcggcgg cttgatgtct acccagccga cgcaactgct
    31321 gaacttcgat cccagcacta tgcaatgggc gacctcttcg ccggtgccgt ccaccttcat
    31381 tggcgcgctg attatttcta tcgttgccgg ttaccttgtg aaatggatga accagaaaat
    31441 ccagttaccg gatttcctgt tagcgttcaa aaccacattt ttgctaccga ttctttccgc
    31501 catttttgtc atgctggcga tgtactacgt catcaccccg tttggcggct ggatcaacgg
    31561 cggtatccgt actgtgctga ctgccgcagg tgagaagggc gcgctgatgt acgcaatggg
    31621 gatcgccgcg gcgacagcaa tcgaccttgg tggcccgatc aacaaagccg caggtttcgt
    31681 tgccttcagc tttaccactg accacgtatt accggtcacc gctcgttcta tcgctatcgt
    31741 tattcctccg attggtctgg gtctggcgac cattattgac cgtcgtttaa ccggcaaacg
    31801 cctgttcaac gctcaacttt atccgcaggg taagaccgcg atgttccttg cctttatggg
    31861 gattagtgag ggtgcgattc cgtttgcgct ggaaagcccc atcaccgcca ttccgtcgta
    31921 tatggtcggc gcgattgtcg gctcaaccgc cgctgtctgg ctgggcgcag tgcaatggtt
    31981 cccggaatcc gctatctggg catggccgct ggtcactaac ctgggcgtct atatggcggg
    32041 gatcgcgctg ggggcagtca taactgcgct gatggtggtg ttcctgcgtc tgatgatgtt
    32101 ccgtaaaggc aaattgttaa tcgatagcct gtaagaagga aaatccagat gacattactc
    32161 gcttcgctgc gcgactggct taaggcgcaa caactggatg cagtgcttct ctcctcacgg
    32221 cagaacaaac agccgcatct ggggatctcc accggatcag gctatgtggt tattagccgt
    32281 gaaagtgcgc acattctggt agattcgcgc tatttcgttg aggtggaagc ccgtgcgcaa
    32341 ggctaccagc tgcatttgct tgacgcgacg aacacgctta ccactatcgt caatcaaatc
    32401 attgccgatg agcagttgca aacgctcggt tttgaaggcc agcaggtgag ttgggaaacc
    32461 gcgcaccgct ggaagtctga actcaatgcg aaactggtta gcgccacgcc ggatgtgctg
    32521 cggcaaatca aaacgccaga ggaggtggag ataatccgcc tcgcctgtgg gattgccgat
    32581 cgtggagcgg agcatattcg ccgctttatt caggcgggga tgagcgagcg cgagatagcc
    32641 gctgaactgg agtggtttat gcgccagcag ggcgcagaaa aagcctcttt tgacaccatt
    32701 gtcgccagcg gctggcgcgg ggcgctgccg cacggcaaag ccagcgacaa gattgttgca
    32761 gcgggcgagt ttgtcactct cgattttggt gcgctgtatc agggctactg ctctgatatg
    32821 acgcgcacct tgctggtgaa tggcgaaggg gtgagcgccg aatctcaccc gctgtttaac
    32881 gtctaccaga ttgtcctgca ggcacagctc gcagcaatct ctgcaattcg ccccggcgtg
    32941 cgctgccagc aggttgacga tgccgcgcgc caagtgatta ccgaggcagg ttttagccac
    33001 tatttcggtc ataacaccgg tcatgctatc ggcattgaag ttcatgaaga tccgcgtttt
    33061 tcaccgcggg acaccacgac gctacagcca ggcatgttac tgaccgtgga gccggggatt
    33121 tatttgccag ggcaaggggg cgtgcgcatc gaagatgttg tgctggttac cccgcaaggc
    33181 gcagaagtgc tctacgccat gccgaaaaca gtgttgctca cgggagaggc ataatggatt
    33241 tatcgctatt aaaagcgttg agcgaggcag atgcgatcgc ctcctcggaa caggaagtgc
    33301 ggcagatcct gctggaagaa gcggatcgcc tgcaaaaaga agtgcgattt gatggtctgg
    33361 gatcggtgct gatccgcctc aatgaatcga caggtccgaa ggtgatgatc tgcgcccata
    33421 tggacgaagt gggatttatg gtgcgtagca tctcccgcga aggggcaatt gatgtgctgc
    33481 cggttggcaa cgtgcgcatg gctgcccgcc agctgcagcc ggtgcgcatc accacccgtg
    33541 aagagtgcaa aattccaggc ctgcttgacg gcgaccggca ggggaatgac gtcagcgcca
    33601 tgcgcgtgga cattggtgcg cgctcgtatg acgaagtgat gcaggcggga attcgtccag
    33661 gcgatcgcgt cacctttgat acaacttttc aggttctccc tcaccagcgg gtgatgggga
    33721 aagcctttga tgatcgcctc ggttgctatc tgctggtgac gttactacgt gagctgcacg
    33781 atgccgaact gcctgcggag gtgtggctgg tggcaagttc cagcgaagag gtgggattac
    33841 gcggtgggca aaccgctacc cgcgcggtgt cgccggacgt cgccattgtg ctggataccg
    33901 cctgctgggc gaaaaacttt gattatggcg cggctaacca tcgccagatt ggtaacggcc
    33961 cgatgctggt gttaagcgac aagtcactga ttgcgccgcc aaaacttacc gcctggatcg
    34021 aaaccgtggc ggctgaaatt ggcgtaccgt tgcaggcaga tatgttcagc aacggcggca
    34081 cggacggcgg agcggtgcac ttaaccggta ctggcgtccc cacagtggta atggggcctg
    34141 ccacccgcca cggacattgc gcggcatcga ttgccgattg ccgcgacatt ttgcagatgg
    34201 agcaactttt atctgccctt attcaacgtc ttacgcgtga gacggttgtt caactgacgg
    34261 atttcagatg atctcctgat taacccggag cggttatgtt aacgattcaa tttctctgtc
    34321 ctttgcctaa cggtctacat gctcgtccgg cgtgggaact taaagaacag tgcagccagt
    34381 ggcaaagcga aatcaccttt ttcaaccatc gccagaacgc aaaggccgat gctaaaagct
    34441 cgctggcgtt gattggaact ggcaccttat ttaatgacag ttgcagcctg aacattagcg
    34501 gcagcgatga agagcaggcg cggcgcgtgc tggaagagta catcctggtg cgctttatcg
    34561 acagcgacag cgttcagccg acgcaggcag aactgacggc gcatccgctg ccgcgttcat
    34621 taagccgcct taacccggat ttactgtacg gcaatgtgct ggcaagcggc gtcggcgtgg
    34681 gtacgctgac cctgttacaa agcgacagcc tcgacagtta tcgggcaatc cccgccagtg
    34741 cgcaagattc cacccggctg gagcacagcc tggcaacgct tgccgagcaa ctgaaccagc
    34801 aattgcgtga gcgtgacggc gaaagcaaaa ctatcctcag cgcccatttg tcgctgattc
    34861 aggatgatga attcgcgggc aatatccgtc gtctgatgac agaacagcat caagggctgg
    34921 gggcggcgat catcagcaat atggagcagg tttgcgccaa actttctgct tctgccagtg
    34981 attatctgcg cgaacgtgtt agcgacattc gcgatattag cgaacagttg ctgcatatca
    35041 cctggccgga actgaagccg cgcaacaatc tggtgctgga aaaaccgact attctggtag
    35101 ccgaggattt aaccccaagc cagtttttga gcctcgattt aaaaaatctt gcaggcatga
    35161 ttctggagaa aactgggcgc acctcgcata ccctgattct gacccgcgcc tcggcgatcc
    35221 cggtactgag cggcctgccg ctggatgcga ttgcccgtta tgccgggcaa ccggcggtgc
    35281 ttgacgccca gtgcggcgtg ctggcgatta acccgaatga cgcggtgagc ggttattatc
    35341 aggtcgcaca gacgttggcg gataaacgcc aaaaacaaca ggcgcaggcc gccgcgcagc
    35401 tggcctattc ccgtgataac aagcgtattg atattgcggc gaatatcggc accgctctgg
    35461 aagcgccagg cgcgtttgcc aacggcgcgg aaggcgttgg actgttccgt actgaaatgc
    35521 tctatatgga tcgcgacagc gcgccggacg agcaggagca atttgaagcc taccagcagg
    35581 tgctactggc agcgggcgac aagccgatta tcttccgcac aatggacatc ggtggcgata
    35641 aaagcattcc ttatctgaat attccccagg aagagaaccc gttcctcggc tatcgcgcgg
    35701 tacgtattta cccggaattt gctggcctgt tccgcactca actacgggcg attttgcgcg
    35761 ccgccagttt tggcaacgcc cagttgatga tcccgatggt ccacagtctc gatcagatct
    35821 tatgggtgaa gggcgagatc caaaaagcga tcgttgagct taagcgcgat ggcctgcgtc
    35881 atgcagagac gattacgctg gggatcatgg tggaagtgcc gtcggtgtgc tacatcatcg
    35941 atcacttctg cgatgaggtc gatttcttca gtatcggctc caacgatatg acccagtatc
    36001 tgtatgcggt cgatcgtaat aacccgcgcg tatcgccgct gtataacccg attacgccat
    36061 cgttcctgcg catgttgcag cagattgtta ccactgcgca tcagcggggc aaatgggtag
    36121 gcatttgcgg tgaactgggc ggtgaaagcc gttatctgcc gctactgctt gggctgggcc
    36181 tggatgagct gagtatgagt agcccgcgta ttccggcggt gaaaagccag cttcgtcaac
    36241 tggatagcga ggcgtgtcgg gaactggcgc gtcaggcatg tgaatgccgc agtgcgcagg
    36301 aaattgaagc gttactcacc gcctttacgc cggaagaaga cgttcgccca ctgctggcgc
    36361 tggagaatat ctttgttgat caggatttta gcaataaaga gcaggcgatc cagttcctgt
    36421 gcggcaacct cggcgttaac gggcgcactg aacatccgtt tgagctggaa gaagatgtct
    36481 ggcagcggga agagattgtc accaccggcg ttggttttgg cgtagcgatc ccgcacacca
    36541 aatctcagtg gatccgtcat tccagtatca gcattgcccg gctggcgaaa ccggttgact
    36601 ggcagtcaga aatgggcgaa gtcgaactgg tgatcatgct gacgctgggt gctaacgaag
    36661 ggatgaatca tgtgaaagtc ttctcgcagc tggcgcgtaa actggtgaat aaaaacttcc
    36721 gccagtcgtt gtttgccgcg caagatgcac aaagtatcct gacgctgctg gaaacagaat
    36781 taaccttctg acgttagccc tgaaaacggg cgctgtactc tcccggcgtc agaccaaact
    36841 gacgccggaa aacgcgacaa aaatagtcgc tatccggaaa accgcaacgc tgtgccactt
    36901 catgaattga cagatgatat ttctgcaaaa tcatcctcgc cttcgccatt cgcacccaac
    36961 gcacatactc gataaaacgc atcgttccat gctgagcaaa cagttttgac agatgattgg
    37021 gcgtgatatt aaaaaactgc gccacgctct cgcgggtgag cggctgggca taattatcct
    37081 gaacccagtt acaaatgctg tgatagagaa aatcgccgcg cggttgcgaa ttacccggcg
    37141 gcatattcac cacgttgcgg caaagatgca gcaaacttaa caccagtggc tgaatgatat
    37201 tctgctccag cggcgaacgg ctaagatggg tgagggcggt aagcatcgct tcaccttcac
    37261 cgcgttgcag gctgggaagc tggatctggc gagcaggatg caacaacgac gtggtgcggc
    37321 tatcgtaaaa cgataaccca agccaggtcg gtgcgaacac caggcttaac agcatcaccg
    37381 gtttgttgtt gatcggtaaa ttagccgcgc gtgccggaat aaacagcatt tctccttcca
    37441 ccattcgctg ctgctttgct tccagcaggt tgccgtattc gccgcgcagc acgatatcca
    37501 gacggggaaa atcaatacat aaactgccca ccggtaacga ggcaggctgg ctggcaaacc
    37561 agacgcgccc cagtagttgc gggttaagca ccaggccgct gaacagatcg gcgaaaaatt
    37621 gttgatcggc gggcaagcct ggtgctttca tgaaagttcc cgattaaaga tgcattaact
    37681 ggcgaaattc tttcactttg ctgcgactga ccggcacttc aaaatccaga tctttcagtc
    37741 gcagaatgta ggtgttatta aaccacggtt cgatttcgcg tattttgttc agattgacac
    37801 aaaatgagcg atggcagcgg aaaaaatgcg acggcggcag tttgctgcaa aattcggtaa
    37861 tgttcatcgg cattacgtag gattcacgcc gcgtatagac aaaggtcatt ttctcgtgcg
    37921 cttcggcgta atagatatcg ttaattggcg tgacgattat tcgctcatct ttcaccagat
    37981 taatggtgtc attctcacgc gttaccgtcg cggcaggcgt actgctggtc tgctattgtt
    38041 gccaggccgc ttccagtttt tgcagcatcc cggtaatacg tgactcctga tacggtttga
    38101 gaatgtagtc aaacgcctcc agttcaaacg cttctaccgc atgttctttc cacgcggtga
    38161 tgaacacaat aaacggttta tgggcgaact ggctgatgtt ttgcgccagc aacacgccat
    38221 ccagcgacgg aatattgata tccagaaaaa tggcgtcgac gcggttatgc tgcaaaaact
    38281 tcaacacgtc cagaccgtcg tcaaaggtgc cgacaatctc catctggctg tgctctttaa
    38341 ttagccagct cagttcctgt tgtgccagga attcgtcttc aacaatgatg actttcacaa
    38401 tatcactccg gctcaaagca ataacgtagc ctgtgaggcg actggggtgc gttggttagg
    38461 aatgtaaaac gcaatttccg tccccggctc caggcggcgg atatgcagcc cctcgccata
    38521 caataacttc acgcgatgat ggacattcag caggccaatt ttattgcccg gcatttcatt
    38581 cgcttcgacc cgctcaatca cctttggatc gatgccgtgc ccggtatctc gcaccgcaat
    38641 gcgtacccga tttccgcact ctgcaacgct gatggtgaca acgcctttac ctttgcaagg
    38701 ctgaataccg tggacaatgg cgttctccac caacggctgg atcagcaggc tgggaatgca
    38761 gcaattcacc tcttcatcaa tatcatagat aaccgtcagc ttgtcaccaa agcgggcctg
    38821 ctcaatggca atatagtctt taatttgata cagctctttt ttgatatcga tttgctcatc
    38881 gtcttttaat tcaatgttat agcgcagata acgcgacaga ttaaagatca actggcgagc
    38941 ggtatccgga ttcagacgga ttgacgatga aatagcgttc agagcgttaa acagaaaatg
    39001 gggattaatt ttgctttgca gggcgcgaag ctctgcctta tttgccattt cacgtagctg
    39061 ctcggcgcgt gaaacctcca gttgcgtgga gatgatttgc gacagaccga ccgccatttc
    39121 ctgtaatgac gaggtgatct gatgcgcgtg gcagtagtaa attttcagcg ttccggtcac
    39181 gacccctttc tcccacaatg ggatcaccag catggaatga atctctggtg tgcggtgggc
    39241 ttcatcattg tttttaatga tgatttttcc gtaattcatc gcctgacggg tagtcgggct
    39301 aatgaagtca tcgccattct gatagttatg ttcacccacg ccaacatagg ccagcacatg
    39361 atcggtatta gtaatcgcca ccgcatcggc gtgaatgtca tcgcggataa tttcgcagac
    39421 cttgcgtaat gactcgctat tgacatggcg aaacagcggt agcgttttgt tggcgatatc
    39481 cagcgccagc ttggcctgcc gcgcggcgct ggcctctttt tcgccctcaa cgctttgcac
    39541 cagaagcaca ataaagccga tacagacgct accgaggatc attggaatgc cgattttaga
    39601 gacgatatcg atccccaacg cggtagttgg tgcccaaaca attaccagaa tcatggtcaa
    39661 cgtctcacac aacatgccgc cgagaatacc gacgcgccag cgctgtgctt tggggatttt
    39721 caggttgatc cagcccgata tacaacccgc cagaatgctg gtgataaagc aggggatcgc
    39781 cgtcacgccg ccgatatcaa ttaaataccg gtgaataccc gcaatcacgc cggtaatgat
    39841 gcctacccac gggccaaaca gaatcccacc ggacatcacc gcgataatac gtacgttcac
    39901 cagcgagcct tctacgggaa cgccggacca ggtgctgaac agggcgaaca gcgagaaaat
    39961 ggcggtaacg gcgagcaatt cttttgggga gtgcgccgac ttgtgcaaca gttcgcgaaa
    40021 caggcggata cggatgagaa agaacaggca gataagcatt aacgctgccc gatcgaagac
    40081 cgccagcagc atgttgaata tttcgtgcac gggtgactct gaataaggat taaacctcta
    40141 tgataggtaa cctgaaggct gatgaccagc aggccgtttt tgaggaaaaa ccataaattt
    40201 aaaaggggat tgaaagataa atggtgtatt a
//
LOCUS       DAZGTR010000029        39866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGTR010000029 DAZGTR010000000
VERSION     DAZGTR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.029"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            69..1124
                     /gene="phoE"
                     /locus_tag="ACHLCY_001708"
     CDS             69..1124
                     /gene="phoE"
                     /locus_tag="ACHLCY_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0368987.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1163..1564)
                     /gene="crl"
                     /locus_tag="ACHLCY_001709"
     CDS             complement(1163..1564)
                     /gene="crl"
                     /locus_tag="ACHLCY_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0368988.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1622..2866)
                     /gene="frsA"
                     /locus_tag="ACHLCY_001710"
     CDS             complement(1622..2866)
                     /gene="frsA"
                     /locus_tag="ACHLCY_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0368989.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2958..3416)
                     /gene="gpt"
                     /locus_tag="ACHLCY_001711"
     CDS             complement(2958..3416)
                     /gene="gpt"
                     /locus_tag="ACHLCY_001711"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0368990.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3677..5134
                     /gene="pepD"
                     /locus_tag="ACHLCY_001712"
     CDS             3677..5134
                     /gene="pepD"
                     /locus_tag="ACHLCY_001712"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0368991.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5191..5805)
                     /gene="prfH"
                     /locus_tag="ACHLCY_001713"
     CDS             complement(5191..5805)
                     /gene="prfH"
                     /locus_tag="ACHLCY_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0368992.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5802..6941)
                     /locus_tag="ACHLCY_001714"
     CDS             complement(5802..6941)
                     /locus_tag="ACHLCY_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP0368993.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7187..7639)
                     /locus_tag="ACHLCY_001715"
     CDS             complement(7187..7639)
                     /locus_tag="ACHLCY_001715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0368994.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7636..8691)
                     /gene="dinB"
                     /locus_tag="ACHLCY_001716"
     CDS             complement(7636..8691)
                     /gene="dinB"
                     /locus_tag="ACHLCY_001716"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP0368995.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8762..9685)
                     /gene="lafU"
                     /locus_tag="ACHLCY_001717"
     CDS             complement(8762..9685)
                     /gene="lafU"
                     /locus_tag="ACHLCY_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP0368996.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            complement(9688..10551)
                     /gene="motA"
                     /locus_tag="ACHLCY_001718"
     CDS             complement(9688..10551)
                     /gene="motA"
                     /locus_tag="ACHLCY_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0368997.1"
                     /translation="MQKILGLLVVFGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(10564..11280)
                     /locus_tag="ACHLCY_001719"
     CDS             complement(10564..11280)
                     /locus_tag="ACHLCY_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFP0368998.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(11300..11767)
                     /locus_tag="ACHLCY_001720"
     CDS             complement(11300..11767)
                     /locus_tag="ACHLCY_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFP0368999.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(11775..12836)
                     /locus_tag="ACHLCY_001721"
     CDS             complement(11775..12836)
                     /locus_tag="ACHLCY_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0369000.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTQELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSFGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(12833..13144)
                     /locus_tag="ACHLCY_001722"
     CDS             complement(12833..13144)
                     /locus_tag="ACHLCY_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFP0369001.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13149..13541)
                     /gene="fliS"
                     /locus_tag="ACHLCY_001723"
     CDS             complement(13149..13541)
                     /gene="fliS"
                     /locus_tag="ACHLCY_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0369002.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(13564..14880)
                     /gene="fliD"
                     /locus_tag="ACHLCY_001724"
     CDS             complement(13564..14880)
                     /gene="fliD"
                     /locus_tag="ACHLCY_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0369003.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15156..16070)
                     /gene="lafA"
                     /locus_tag="ACHLCY_001725"
     CDS             complement(15156..16070)
                     /gene="lafA"
                     /locus_tag="ACHLCY_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HFP0369004.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            16556..17398
                     /locus_tag="ACHLCY_001726"
     CDS             16556..17398
                     /locus_tag="ACHLCY_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFP0369005.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPDSSPAKKLIITDQIQETVPEEISSTTPPVPVRKKHKVIMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLSSGKENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     LQKC"
     gene            complement(17453..18430)
                     /locus_tag="ACHLCY_001727"
     CDS             complement(17453..18430)
                     /locus_tag="ACHLCY_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HFP0369006.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQNTALMQML
                     DKRTALSGGAVDRQLQPVLQGEARVTFHSPDQANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQLHQQADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSEADRLAQSLQQGGAGISQTLESINQQRAQMAVQQEKARQLIDGMS
                     RFPQTENAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(18447..19376)
                     /gene="flgL"
                     /locus_tag="ACHLCY_001728"
     CDS             complement(18447..19376)
                     /gene="flgL"
                     /locus_tag="ACHLCY_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP0369007.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKQILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEVTLKTYSKVSELNLFSVL"
     gene            complement(19391..20767)
                     /gene="flgK"
                     /locus_tag="ACHLCY_001729"
                     /pseudo
     CDS             complement(19391..20767)
                     /gene="flgK"
                     /locus_tag="ACHLCY_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(20950..21249)
                     /locus_tag="ACHLCY_001730"
     CDS             complement(20950..21249)
                     /locus_tag="ACHLCY_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HFP0369008.1"
                     /translation="MKVNASGGIDGSDALMGPKVQANDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
     gene            complement(21249..22349)
                     /locus_tag="ACHLCY_001731"
     CDS             complement(21249..22349)
                     /locus_tag="ACHLCY_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP0369009.1"
                     /translation="MQNWIKAAVIAVSLALPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVTVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            complement(22364..23029)
                     /gene="flgH"
                     /locus_tag="ACHLCY_001732"
     CDS             complement(22364..23029)
                     /gene="flgH"
                     /locus_tag="ACHLCY_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP0369010.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPKTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FSHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            complement(23112..23897)
                     /gene="flgG"
                     /locus_tag="ACHLCY_001733"
     CDS             complement(23112..23897)
                     /gene="flgG"
                     /locus_tag="ACHLCY_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP0369011.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTLEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            complement(23976..24713)
                     /locus_tag="ACHLCY_001734"
     CDS             complement(23976..24713)
                     /locus_tag="ACHLCY_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HFP0369012.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGAGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLEKNAEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            complement(24713..25915)
                     /gene="flgE"
                     /locus_tag="ACHLCY_001735"
     CDS             complement(24713..25915)
                     /gene="flgE"
                     /locus_tag="ACHLCY_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP0369013.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVMCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFNASD
                     EAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPTTPQTIEFQTDAAAPVDLTVDYSTCTQYGSEFSVTTN
                     AANGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTAWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            complement(25939..26652)
                     /locus_tag="ACHLCY_001736"
     CDS             complement(25939..26652)
                     /locus_tag="ACHLCY_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HFP0369014.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTQSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSVNPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDVA"
     gene            complement(26652..27083)
                     /gene="flgC"
                     /locus_tag="ACHLCY_001737"
     CDS             complement(26652..27083)
                     /gene="flgC"
                     /locus_tag="ACHLCY_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP0369015.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            complement(27086..27421)
                     /locus_tag="ACHLCY_001738"
     CDS             complement(27086..27421)
                     /locus_tag="ACHLCY_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HFP0369016.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            27503..28240
                     /gene="flgA"
                     /locus_tag="ACHLCY_001739"
     CDS             27503..28240
                     /gene="flgA"
                     /locus_tag="ACHLCY_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP0369017.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRQWHDYRYTFKIYIPSQIATAAPCTKTPGVTLTSPAEIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMTKSLVARDMPLTANDVELKPYNVSAQRRDVLMELDDAI
                     GLSSKHALQPGRPITKEELVSPVLVGRDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            28321..28599
                     /gene="flgM"
                     /locus_tag="ACHLCY_001740"
     CDS             28321..28599
                     /gene="flgM"
                     /locus_tag="ACHLCY_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP0369018.1"
                     /translation="MKITPTMPGNRPTATTAGSSQPRKTTVTTTTTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSNKVAQVQAMLASGSPQVDTEQLASDMLSFFQN"
     gene            28612..29040
                     /gene="flgN"
                     /locus_tag="ACHLCY_001741"
     CDS             28612..29040
                     /gene="flgN"
                     /locus_tag="ACHLCY_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HFP0369019.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEEL
                     LAVNDEITHHYEQLHGHSHQRHSLLKMLGVSVNRDGLAQVFTWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQSFLGSEADFLYQE"
     gene            29167..30096
                     /locus_tag="ACHLCY_001742"
     CDS             29167..30096
                     /locus_tag="ACHLCY_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HFP0369020.1"
                     /translation="MSFIECYEPEYVRKFLARHPGSPLYVRKVWKNEIRLSLSLTDIA
                     SCEAVLNDVRAFDLQLTYRPVEDNSAELSARDAIVNQVILSTLTLRDLTPELSLYAVG
                     ILLSCASKMPGRDGDILARLTTLPQALADHAKKGTLQAQYAQLPPVPQLARQLMTLLG
                     GCAFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQTQWQTTWQQHFATAPWM
                     MRNWLIYRVYHDMIGQTDGADYFPLVCDFYLLRTLISLWTLDGSPLRQEDIFALFAMF
                     ERWRASENALLVRQQIQSLCAADPLLSAFSLLT"
     gene            30275..32719
                     /locus_tag="ACHLCY_001743"
     CDS             30275..32719
                     /locus_tag="ACHLCY_001743"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0369021.1"
                     /translation="MPTPTIIKTLATHREKPFVSVVTPTWNRGAFLPYLLYMYRYQDY
                     PADRRELIILDDSPQSHQHIIDRLTNGTPEAFNIRYIHHPEKLPLGKKRNMLNELARG
                     EYILCMDDDDYYPADKISYTIAMMQKHRALISGSDQIPIWYSHINRIFQSRSFGPHNI
                     LNGTFCYHRNYLKKHRYDDDCNLGEEKSFTDNFSVNPLQLPGERTILCISHSHNTFDK
                     DFIIGASTPVNTALTDIVRDPLLRNAYLSLHNATHHQAINHQAIDQVVVVNLDKRPDR
                     LQQIREELALLHIPPEKITRLAASEDENGQRGRRQSHLQALRLAQQHGWQNYLLLEDD
                     AVILKQEKHIQVLNTLLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAY
                     LVNSRYYPQLAQQMSNDEHSLEDCWQPLLRADKWLACYPSLCYQRPGFSDIQKKTTDN
                     IARYFNKLPVATKPSALPIADTIGFFMETSFHYALYRPIITALQAQGLSCTLVINDRV
                     FKPFLDEMLETLKNIDDPQLKGMRLSEMQTHGQRVKCLVSPYHTPALNGLAAVNIRAM
                     YGLAKETWNHADWNRFYQSILCYSHYSQQALAHFGSAKVVGNPRFDAWHNGTFDRALP
                     ENIQSDYRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLICKLHHGTCSRPEEAASL
                     ALVRRHLKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRVILLDFPGMNDLLD
                     GEKSYSTAESADQRIREILPVAHDVAELRYLLSEAFDWGSVQARLTEIRHHYCDAFMD
                     GKAGERAAIVIVEALG"
     gene            complement(32837..33232)
                     /locus_tag="ACHLCY_001744"
     CDS             complement(32837..33232)
                     /locus_tag="ACHLCY_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFP0369022.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARALGERLLVGVSSDALNIAK
                     KGRAPVYHQDDRMAIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDS
                     FAYICEVVYFPRTPSVSTTGIIEVIRGLL"
     gene            complement(33235..33669)
                     /gene="fliJ"
                     /locus_tag="ACHLCY_001745"
     CDS             complement(33235..33669)
                     /gene="fliJ"
                     /locus_tag="ACHLCY_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP0369023.1"
                     /translation="MRQIIDTLTQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAVSVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAVDDIAVQCWLRHKLAE"
     gene            complement(33672..35009)
                     /gene="fliI"
                     /locus_tag="ACHLCY_001746"
     CDS             complement(33672..35009)
                     /gene="fliI"
                     /locus_tag="ACHLCY_001746"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP0369024.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDFLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSLEHQQSARALKQNYAAYMEIKPLIPLG
                     GYVAGADASVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPGVKKAEQ"
     gene            complement(35002..35712)
                     /gene="fliH"
                     /locus_tag="ACHLCY_001747"
     CDS             complement(35002..35712)
                     /gene="fliH"
                     /locus_tag="ACHLCY_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP0369025.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTTSISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGRKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALKETLTPESQGE"
     gene            complement(35715..36725)
                     /locus_tag="ACHLCY_001748"
     CDS             complement(35715..36725)
                     /locus_tag="ACHLCY_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP0369026.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNCFQGNQQVI
                     LDQMRERDEEVLEQLQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAEQT
                     AE"
     gene            complement(36703..38349)
                     /gene="fliF"
                     /locus_tag="ACHLCY_001749"
     CDS             complement(36703..38349)
                     /gene="fliF"
                     /locus_tag="ACHLCY_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001643010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP0369027.1"
                     /translation="MNAQIKKLTQAFPAFRLRLADNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEEALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSRQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLHASKVSVVDQ
                     SGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTHASVANVLDSLVGSGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPVAANQMTNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTAQ
                     LTALLNNAAGIDAQRGDNLTLSLLNFVPQAVPVEPIIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPVMKRLTAVRAPIITPESEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            complement(38354..38695)
                     /locus_tag="ACHLCY_001750"
     CDS             complement(38354..38695)
                     /locus_tag="ACHLCY_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP0369028.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     HRIMQGALGHVDQFQQVAEQQQTAIDMGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            complement(38710..39696)
                     /locus_tag="ACHLCY_001751"
     CDS             complement(38710..39696)
                     /locus_tag="ACHLCY_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HFP0369029.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENENAPYIGVNCAAIPENMLEATLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQEVAAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYSTVLVKNITLSESTRRAMLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPENDIADHSELQWPAQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKHGIEVFS"
BASE COUNT         8645 a        11121 c        10508 g         9592 t
ORIGIN      
        1 aaactttcct gttttcaacg gggctctccc gctgaatatt cgcgcgttaa ttaaaatcag
       61 gaatgaaaat gaaaaagagc actctggcat tagtggtgat gggcattgtg gcatctgcat
      121 ctgtacaggc tgcagaaata tataataaag acggtaataa actggatgtc tatggcaaag
      181 ttaaagccat gcattatatg agtgataacg ccagtaaaga tggcgaccag agttatatcc
      241 gttttggttt taaaggtgaa acacaaatta acgatcaact gactggttat ggtcggtggg
      301 aagcggagtt tgccggaaat aaagcggaga gtgataccgc acagcaaaaa acgcgtctcg
      361 cttttgccgg gttgaaatat aaagatttgg gttctttcga ttatggtcgt aacctgggcg
      421 cgttgtatga cgtggaagcc tggaccgata tgttcccgga atttggtggc gattcctcgg
      481 cgcagaccga caactttatg accaaacgcg ccagcggtct ggcgacgtat cggaacaccg
      541 acttcttcgg cgttatcgat ggcctgaact taaccctgca atatcaaggg aaaaacgaaa
      601 accgcgacgt taaaaagcaa aacggcgatg gcttcggcac gtcattgaca tatgactttg
      661 gcggcagcga tttcgccatt agtggggcct ataccaactc agatcgcacc aacgagcaga
      721 acctgcaaag ccgtggcaca ggcaagcgtg cagaagcatg ggctacaggt ctgaaatacg
      781 atgccaataa tatttatctg gcaacttttt attctgaaac acgcaaaatg acgccaataa
      841 ctggcggctt tgccaataag acacagaact ttgaagcggt cgctcaatac cagtttgact
      901 ttggtctgcg tccatcgctg ggttatgtct tatcgaaagg gaaagatatt gaaggtatcg
      961 gtgatgaaga tctggtcaat tatatcgatg tcggggctac atattatttc aacaaaaata
     1021 tgtcagcgtt tgttgattat aaaatcaacc aactggatag cgataacaaa ttgaatatta
     1081 ataatgatga tattgtcgcg gttggcatga cctatcagtt ttaatgatta ttgccggatg
     1141 tgatgcatcc ggcacatttc actcacgccg ttaacttcac cggctcgtca cgaaaatcat
     1201 ccgccggttc cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc tcgtgaaact
     1261 cacgcagggt gtgctccagt cgttcaacca cttcagtgtc ttttaccgga acactcttcc
     1321 agtcgcctgc tttatcgaac agaccaaact ggtaactgta ggtaaaacgg gattcctgcg
     1381 cttcaagctc catccaccag ccccagaact cacgcacttc cggtgccggt ttcacgttga
     1441 cgcatacagc cagacaatcg aaaaagaatc gattatcttc gcacttacct tcacgaatat
     1501 acgggcctag tgcggtaaat tttttgatca atctgctctt cgggtgtcca ctcggtaacg
     1561 tcattgctat ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat ttagcaaatt
     1621 tttaacacaa gcgtttttcg atccagccgg tgatttcctg aagacctttg tcaaaattcc
     1681 gatacaccgg gttaaatggg atctctaata atttaccgtc agcagatgat gaggtgatta
     1741 agcgtgagtc ctcttccggg ctgaacggat cgttcttcca gtagcctgat aacattggcg
     1801 ttgggcagcg acgccccagc aatccttgca cttttaatga atagcgattc agctccacgc
     1861 gtaacgcgtc atcggaagca tcatgcatcc ccaaacgact cgccagaacg tcaagataca
     1921 tttccggcac ctgttgctgg cacttaaaat cactcaacag ggtatgaact accggaccaa
     1981 gacaggcaac cgctttcaga cgcggcgatt caagatatgc cagacgcacg gcaacgttag
     2041 cgccgaaacg gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt acgttaggca
     2101 gcgcctttaa gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc cattttgaag
     2161 aaaagcccac cgatggcata tcaatagtca gcatcgcaat gccgcgcggc gcaaaataac
     2221 gttcatacag gctgtaatag tccgtctgca tcgcatccag accaccacac attaatactg
     2281 tcgggaacgg gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc gcaccgccgg
     2341 gtacggtaaa ctccatctgc cgcatcgtgc ccggcagacg ctgggcggcc tcttcatagg
     2401 cgcggtttga taaagcctgc gcttgctcgg ccaggtcatc tcctttcaga tgaggatagg
     2461 cggcaatgtt gtacaacgta gccgcatgca gccagtgtcg accactgagt tgcggatctt
     2521 cttccgcaca ggctttttgt tgccacacca tcgcctgggt ggcccactca taaatccagt
     2581 tgccaccacg gtagccaatc acagtatcgt ataaatcatc gtcggtacgt tcagcatcgc
     2641 tcatcacaat acgtgcctgg acgtcgagga tttcacgtgg gtcaataccg cgccagatcc
     2701 acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca agagccgact
     2761 gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt
     2821 taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca
     2881 gaatacttac tggaaactat tgtaacctgc ctgaagttaa aaagaacaac gcccggcagt
     2941 gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc
     3001 cacggctgtt cgatccaggt atcttgcggg atatcaacaa catagtcatc aaccagcgga
     3061 cgaccagccg gttttgcgaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc
     3121 gcaacagcag taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg
     3181 ccttctgcgc gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata
     3241 caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtaccaga
     3301 ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag tcggcttgcg
     3361 agtttacgtg catggatctg caacatgtcc caagtgacga tgtatttttc gctcatgtga
     3421 agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat aggttgcgcg
     3481 agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc gtctttttac
     3541 gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc gcaagcctgt
     3601 cttgtgttga caacattttc tgctaaccct gtgacctgca atactgtttt gcgggtgatc
     3661 gacaaggaga cttaacgtgt ctgaactgtc tcaattatct ccacagccgc tgtgggatat
     3721 ttttgccaaa atctgttcta ttcctcaccc gtcctatcat gaagagcaac tcgctgaata
     3781 cattgttggt tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg taggtaatat
     3841 cctgattcgt aaacctgcta ccgcaggtat ggaaaatcgt aaaccggtcg tcttacaggc
     3901 ccacctcgat atggtgccgc agaaaaataa cgacaccgtg catgacttca cgaaagatcc
     3961 tatccagcct tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc tgggtgcaga
     4021 taacggcatt ggtatggcgt ctgcgctggc ggttctggct gacgaaaacg tggttcacgg
     4081 cccgctggaa gtgctgctga ccatgaccga agaagccggt atggacggtg cgttcggctt
     4141 acagagcaac tggttgcagg ctgatattct gattaacacc gactccgaag aagaaggtga
     4201 aatctacatg ggttgtgcgg ggggtatcga cttcacctcc aacctgcatt tagatcgtga
     4261 agcggttcca gctggttttg aaaccttcaa gttaacctta aaaggtctga aaggcggtca
     4321 ctccggcggt gaaatccacg ttggcctggg taatgccaac aaactactgg tgcgcttcct
     4381 ggcgggtcat gcggaagaac tggatctgcg ccttatcgat ttcaacggcg gcacactgcg
     4441 taacgccatc ccgcgtgaag cctttgcgac cattgctgtc gcagctgata aagtcgacgt
     4501 cctgaaatct ctggtgaata cctatcagga gatcctgaaa aacgagctgg cagagaaaga
     4561 gaaaaatctg gccttgttgc tggactctgt agcgaacgat aaagctgccc tgattgcgaa
     4621 atctcgcgat acctttattc gtctgctgaa cgccaccccg aacggtgtga tccgcaattc
     4681 cgacgtggca aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga ccatgactga
     4741 caataacgta gaaattcact gcctgatccg ttcactgatc gacagcggta aagactacgt
     4801 ggtgagcatg ctggattcgc tgggtaaact agctggcgcg aaaaccgaag cgaaaggcgc
     4861 atatcctggc tggcagccgg acgctaattc tccggtgatg catctggtac gtgaaaccta
     4921 tcagcgtctg ttcaacaaaa caccgaacat ccagattatc cacgcgggcc tggaatgtgg
     4981 tctgttcaaa aaaccgtatc cggaaatgga catggtttct atcgggccaa ctatcaccgg
     5041 tccacactct ccggatgagc aagttcacat cgaaagcgta ggtcattact ggacgctgct
     5101 gactgaactg ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc cggatggcgt
     5161 ttaatcgcct tccggcagtt tcatccttca ttatccttcg ataaaagcca tccctgtaaa
     5221 tgtccgtctc gggttgccac gttcaatctg gtgatggaac attcgccgca gcgatttcag
     5281 caccgcgcta ttttcctgtt gctgctgctc cagcttccag gcaatcagca atcgtgccag
     5341 ccgcttgtta gcatgctgac tacgctctga ctgaaccttc acgctaatac cggatgccaa
     5401 atgcgtggcg cgtaccgccg agtcggtttt attgacatgt tgaccgcccg gccccgacga
     5461 acgcaacgtc tcataacgga ttgcatccga ttgttcctgc tcatcagcgg taaaacgccc
     5521 aatgcccaga aaccagtttt tgcgcccatg atgaggccga tacggactcg gacaaatcca
     5581 ctgaatagtg ccacaccagc tttcgcttaa tgcccatgcg ttatcgccat ccagagaaat
     5641 cagcgccgaa cgcagcgtgt cagagtagtg gcccgtttct gtttccagca ccgttaccgc
     5701 gacgtcttgt cgggcagctt ctttaattag cctgtccagc gcctttttca ctgcgagaca
     5761 acattcttcc ggcccctgcg ctgaggagag ttgtagcaag atcatccttt tttccctcca
     5821 ctgtttttga gcgttagcac cggacgcagc cgcgcgacag gaataattaa accggcctgc
     5881 accagacatt gcaccacgct ttcagccgat ttataggctt gtggcgcttc ttcaaagatg
     5941 agttgtttat cgcgacaaat tacccgactg ccaagttcag tacgcgagag ttgcgtcgcc
     6001 gtgtatttcg ctgccagacg ccctttacac tcggtgcgcc cccatttacg ccctgcccca
     6061 tgcgccagcg agtggagcgt tttttcgttc gcaacaggct gtacaatcca ggagtaatca
     6121 ccacgtgaac cgggaataat caccagaccg ttgtcatccg gcgtggcacc tttacgatgc
     6181 aaccagccct gttgatcgcc gatttgacac gcgctaacaa agttatgcgc cacatccaga
     6241 accggacttc ccgtggcctt aacttgttgc attatgcgca aagcgatcat ctggcgatta
     6301 atacacgcaa acgccagcgc atcgtcatgt tccgcaatat accgtagcgc gtcgtcactg
     6361 ccttgaggca aaccatgatg cgagaacgag gcaatatgcc gctgtaaaat agactgcccc
     6421 accccccgcg agccgctatg aaccaacagt tgcagatgtt gtgcatccag acctgccagt
     6481 gcaaacagtt cagcgttgat gatttgatca acttgttgca gttcagcgaa atgattgccg
     6541 ccaccgatag agccaagcga gctgcgccac ggatgctgtg caaatgctga cggcaggttt
     6601 tcctccagcc agctttcttc agcaacgtca tccagatctg ataatcgctt ttcaaactta
     6661 tcggcgttgt atttgcgagc gagaatatct gtttgccata gcgccatacc gcagccgata
     6721 tcattgccga ccagtgccgg ataaaaacga ccaacagaga agaacgctgc gccaatcgga
     6781 tagccgcgtc cgggatgtaa atctggcatc cccactacgc gctgcatgtt gggtaaattt
     6841 gccgtggtgt gtaattgttg gatcgctaaa ctctcgatcc acaggtcatc agatgcgatg
     6901 gtaaataccg catcggataa gggacgaata taattgccca tataccattc cgtaaataga
     6961 ctcgtcatac ttcaagttgc atgtgttgcg acttgaatta ttgagagtaa caattaaaaa
     7021 atcaggaaat ggcagacgaa tccagggcaa gaaaaaaaga attaatcctg gaaaggtctg
     7081 caaagagtca ttgataaagg atttgtcatg atttacctcc ttgcagtttg tggaataaga
     7141 aaacgcgcac atactaggct tgcgttattt tcattgcaag ctggatttaa tgttgctgtt
     7201 tataacgcat ataaaaatta ataaaccact ctccccgaca ttcaacgcgt tgctgcttaa
     7261 ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt tatgcttgcg tccaccgtaa
     7321 gttcggattc agacttaatc aaaggtttta acaaagcgct ggcaacccca cggcgggtgt
     7381 attcagggtc aacaaataac atatcgatat agtgttcaac gcaggtaata aagccgacca
     7441 gttttgcatt aatgactgca acccgcactt gtgatttcgc gagtttctcc ttccagcgag
     7501 attcgtcaat ctgcgcccag gcggcaattt gttgtggtga ataatgctga ctggcagtca
     7561 tcgtaaccgc tctaatgaaa atagcgcata gttgctgaaa atcgccaggc tgatagtctc
     7621 ttatttgtat gttattcata atcccagcac cagttgtctt tccatttgcg ggtcaagcaa
     7681 cgtcacatgc agccccacca gtcgcacacc gcgcccgccg cggcgttcat cccaggtttt
     7741 acgcgcggtg gcgattaagt cagctttatt cagccgcggc cagacgtgct cctgagtggt
     7801 ttgctgaaaa tcatcaaact ttaatttcac tccctgacgg gcgatcagta aatcaggttt
     7861 tacctttgcc agacggcgtt caagttccgg atacagccgc tcgataatcg cttcacattc
     7921 agaccagtga tgaatatctt ccgccatcgt gcgttccacg ccgacggatt ttcgtaaccg
     7981 ttcgctgtta acgtcgcgct cgtcaatccc ctgactacgc tcccacaaaa tgcggccaaa
     8041 tttgccaaag cgtttaagca gcatcaccag atcacacttt tgtacatcac cgcaggtccg
     8101 cagccccatc gcttccagtt ttgccgccga gactttgccg acgccgggga tttttgccag
     8161 cggtaaggtt tgtaaaaatg ccggaacttc tgccggcgta atcacaaact gaccgttggg
     8221 tttattcatg tcggaggcga ttttggcgag aaactttact ggtgccacgc ccgcagacgc
     8281 tgtcagttgc agctcgctga aaatcgtctg gcggatttcc tgggcgatga gggtcgcaga
     8341 accgtggcaa tggacgctat cggtgacgtc gagataagcc tcatccagtg acaacggttc
     8401 aatacgcgag gtgtagcgcg agaagatttc acggatatga tttgaggctt ctttgtaggc
     8461 gtcaaagcgc cccggaagca aggtgagatg cgggcataat ttgagcgcca tccctgtcgg
     8521 catagcgcta cgtacgccaa atttacgcgc gggataattg gcggtgctga tcaccccccg
     8581 acgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc agggcgggat tgtcgcgcat
     8641 ctccaccgct gcgaaaaagc agtccatatc cacatgaatg attttacgca ttactcacct
     8701 ctcaacactg gtaaagtata cagtgatttc agggtttgag aaatgcgtaa agattcagca
     8761 tttaacgcgt tcgctgcgaa agcacctgct gcttatccag cttttgcacc agcggctgca
     8821 ccactttatc cccatgctga ccaaagtatt gatacagcgt atcggacgca cttttggtca
     8881 gcaccataat ctcaatgcgc cggttgcccg cgctttgcgg atttttggaa tccagcagca
     8941 tctggtccgc cattgcgctt acctgcatca ctttatcttc cggcattccg gcctcttcca
     9001 gcacccgacg agccgaaagc gcgcggtcac ccgaaaggtt ccagttgttg tagatattgt
     9061 ttttgtaagc catcgcatcg gtatgcccgg taataataat tttgttatcg agcgagtcga
     9121 aaactggcgc gagctccacc agcagcgttt taaagaacgg cataatctgc gcgctgccgc
     9181 gttcgaacat attgcgattc tggtcgtctt taatcagtac gcgtaatccc tggggaacaa
     9241 tctccatctc cagattcgct tccatatgcg catcacgggc aatggtgttg atactggttg
     9301 ccagctcgcc aagctcggtg gcggatttct tcttcagcag cgccgttttc tcattcacat
     9361 cacgcgcttt tttctccgtc tcttccggca ccgccacggt ggcaggtttt ggcggatgag
     9421 acgggctgat tttatttaac ggcgacagcc cgccaccgtt aaaaatcgac tgcccgtgca
     9481 gcgcggcgat aatgctttca cgctcggatt tactgacgct gttgacaatc cacagcgtca
     9541 taaacagcgc catcatcgcc agggtaaagt cggcgaacgc caccttccac gccccgccat
     9601 gatgcccggc atgattcttt ttgatctgcc gccggacgat ggtggttttt gccccacgct
     9661 cgcggcggtt gcccgtcttt ctcatgctta ttcttgctcc agcatcgcat tcacccacgc
     9721 gtcgaggttg gcgaaggtcg gtttggacgc cagatgcagc agcttacgcc cggcatccac
     9781 cgccagcagc gtcggtttgc cgcctgcctg cgccaccagc acagtacgca cgcattccag
     9841 caaagaatgt tctgcccgcg cctgttgttc catggcgttg gcgagcgggt ccatcaggca
     9901 gtagcagata aagacgccta aaaacgtccc caccagcgct gccgccactt tcaggccgat
     9961 cagggcaata gagccgtcaa tcgactgcat ggtgatgatg atccccagca ctgctgcaca
    10021 aataccaaat cccggcatcg cttcggcggt acgttgcagc gagcgcgacg gcgtgagcag
    10081 cgactcttcc gcagtgtcca gttcctgatc gagaatgcct tccagttcat gggcgtcgat
    10141 tttacccatc gccatcaggc gaaagttatc ggcaataaag gtcaccagcc gcttctgggt
    10201 taacaccagc gggtatttct ggaaaatggt gctctcttcc ggttgctcaa tatgttgatc
    10261 cagcatgcgc agaccaccgt tttgcaccat ttcgagcaac tcatacaggc acattaatag
    10321 ctgacgctga aactccggcc cgagctgttt tttactgatc acacctttaa tttgatgggc
    10381 aatctctttc agcacatgtt tcgggttacc aataatcatc gccccgaaac cggcacccaa
    10441 aataatgatc atctccgccg gttgccagat agccactaat tgcccgcccg cttcaaaata
    10501 accgccaaat acgcagccga acaccaccag taaacctaat attttttgca tgatttcttc
    10561 actttaattc ggatagacaa aatcacgaat tttctgcgcg atctgtttat tcagttgaca
    10621 aattcgggcc tcggttagcc ctaataccag cgcaatctct ttgagattca tctcatgctg
    10681 gtaatacatg gagaggatca gttgctcttt ttcgcttaat tgtgaaagcg cctgttgcag
    10741 catttgctga gtgacaaact gatcttccag ctcccggcca acaagcggaa tgcctggccc
    10801 ttcgccgttc agcagttcgt ccaggctggc gagcgtttcc gccccttcca gttgctgata
    10861 ttcgagataa tcttcatgtg agatcccttc cacggccagt tctgaccact ccggttcgtg
    10921 ccccagtttt ttgcgcgttt cgcgaatcag atctttcatc tgatgatatt tctgtcgcag
    10981 ctggcgcggt cgccagtcga gagcgcgcag ctcatccagg atcgcgccgc gaatacggtg
    11041 cacggcatag cccgcaaaac cttcgtccgg cagcccgtag cggcggatgg cattaagcag
    11101 ccccattaac gccgtttgtt ccatatcctg acgatcaata atgcaattac actgcggggc
    11161 aagttgccgc accactttac gcaccaacgg taaataggcc tgcaagtagt ggctctcctg
    11221 ctgcggggtt aataccggcg cggcagcaaa ttcttctgac tcgaatacat cctgtagcag
    11281 gtttatatcc gcctggtgat tactggaaga ccactttttt caccagcaaa tccttatacg
    11341 gtgcgctcat tttgcgacgc tgaagatctt tctttaacgc gtcagaaatg agtttgcgga
    11401 tggcagatat tttcatgccg cgcaaatctt caaatttcat ttccgtcagg cattcaaccg
    11461 caatactttg atacagcggc tcctgatgtt ttattttttc cgcttctttg tcactggcga
    11521 ccaccatcac taattccgcc gacaaataat ggtccgcgcc gttgttgtca tgcagcgtga
    11581 caatggtttc cggcaacgtc acaaacacgc ttttgctttc atccagggtt tccaccgttt
    11641 ccgtctgcgc ggcggcagtc ggttttcctt ttccggcata atgatgccag acaccccatc
    11701 cagctccggc ccccaccacc aacgccagaa cggcgctgac cacgcccgcc accacgattt
    11761 tcttcatatt gcctttacac tttgatcaaa acggattcgt tctgttgaaa ttgaggcgca
    11821 tcatcaagct gtaaggcggc actcacctct tcctgctgat ggcccgactg ttgttgttgc
    11881 tgcgactgcc cttcggagga aacctgtacg ttcacctcca taaaattttg cgccgtcaga
    11941 tgctggcgga gatcgtcgct aaactgctgt aacgcgcggc aaacttcact ttggttcgcg
    12001 ccgatgtgca ccatcagttt gccgttgtcg agttgtacgg cgatctcgag cttgccaaac
    12061 gacggtggat cgagacggat ggtggagatc tgctgttgtt tgttgagctg aaagtggatc
    12121 tgatctttca aaagcgtcgt cagtttttcg cccagttcgt ccatcgaaag cgtggctggt
    12181 ttggcgacaa tctcctgatg atcgctgagc agctcctgcg tcttaacctg caacaccgca
    12241 cgcgcaataa ccggttcact gtccggcact ttttccgttt tacgctcgac ctgcgggcgc
    12301 gcggtcacac gggttagttc agatttcggg cgagctggct gtgttgagac gcgcggctgc
    12361 gtcggcgtaa tggcatgtaa atcttcacta gcgtaagtcg ccagtctggc ctgctgttcg
    12421 ggcttcgcct gcggcaactg actgatcagt tcctgcaact gcggcggcaa cggcgtgagt
    12481 tcctgctgtt gcgtctggcg ttgcggcgcg ttttgcacca ccgctttcgt caattgctgg
    12541 ataaccggcg atgttgcgac gtttcgcggc tgcggcgtgt cctgatgcgg cgcggcaggt
    12601 tgcggaagta acagcgccat tagcgcctgc atcgccaccg gatcgttctc ctgcgggcgc
    12661 gtcgcctgtt gctgactggt ttttgcgcta tgaacgcgct ccgccaccgc cgcgaccgcc
    12721 attttcggca gggtaaacgc gggcgcgttt tcaccgctca ccggcggtaa aatatccgcc
    12781 ttcaacgaag cggtttccgc aagcgttccg agcgtggcaa gcaatgtcgg attcatgact
    12841 tctccgaaag taaattaaca attgagtagg ccagtacgcc ttcctgatgc cgctgatgct
    12901 gttccatacg cgctttcaac acttctgcgc gctgctcgcg ccgtttaatc agggcgttaa
    12961 acgcgtcata cagtcgcgct ttcactgcat ccaactgcgc cgcatcggtt tccgccgccg
    13021 cgtaatcgtg aaactggcgt atcaactgct gatgcagtgc cggaaggcgt cgccagcgtt
    13081 gcttgtcgag ggcttcattc atcgcctcga ccagtgcgaa aagtttcctg cgttgttttt
    13141 ccatcgtctt aacccagctt gcccgaaagc ccttcccagc cttcgcgcag gttgcttaag
    13201 atcagcatca cttcatcgat tttctctgcc gacagttcgc cgctggcctc atacaatcgg
    13261 taaacgcagt ggtcatagag tcgcgaaagg ttgaccacca gctcgccgcc agtttcaaac
    13321 tccagcgaac tggtgagcgc attgagaatg tcgatgcatt tattgatgct ctgggctttc
    13381 ttctcgaaac gccgcccttc aatatggctt ttggcgcgtt ccagttcgtc catcaggcca
    13441 gaaaacagca ccagcaccag ttccagaggc gatgccgccg cagtacgcgc cgcgagatcg
    13501 atctctttat attgcgaata gccgtcctgt acttggtaca tctttaggga tattccttcc
    13561 gagttacgcg aatgccgcca tgctggcttt catggtgtac acctcgacca gcgtgttggt
    13621 gtactcctcg agataacggt cgtagttggc gttataggta tcagtaagct gatcgccttc
    13681 gtcctgaatt ttgctcatct gatcgtcaat gttctgctgg cgcagggtga tgatgccgtt
    13741 gctggagtcc gtgtaggtgt taataaggtc gtccatctgc gccaccatgc tgttgtcgcc
    13801 aacgaaaata gaggtcagac cgtcaggatt tttcgccatt tcgtcgttga actggtcgga
    13861 gtcaatctgt aagtggccgt gagaatcgag ggtgatgcca tagtcaacaa tcgacacgcc
    13921 gttgtagctg gcatgggcga tgtcatcgag ctggttcgcc agtgaactga gtccggcgtc
    13981 gcctgcaaat accccggcgc tggtactgtc atcaccgtgg gtggttagcg aatcaaccgt
    14041 atcaatcaag gtgttatagg catcgacaaa ggtctgtact ttctcctggc tggcgctgga
    14101 atcttcgctg atgttaaagg tagtgagatc gctgtcagaa tcgctgactt cggtgaaagt
    14161 catggtgaca ccggggatca catcatcaaa cgtattgctg ctgttggtga tctcaggccc
    14221 cgtggcgctg ccaagatgaa taatcgcgtc ctgagcggag gaaacatccg tggcaacggg
    14281 cgcgctggtg ctatcgttgc tggcatcatg ccccgttacg ctcaccgaaa acgcgctctg
    14341 cgccccggtg ctatccgagg tgagcataat cgttgtggtg ccgtcggttt ttaccagcgc
    14401 cgccgacacg cccggattat catcggagtc gttaatcgca ttcaccagtt cactggcgtc
    14461 gataaaaccg tcgccgtcac cattctgatc tgccgccgac agatcgatat ccatggtgct
    14521 gtcgcccatc gtcagttcaa aagtgccggt ggcggaaaag gcgtcgtcgc ccatactgaa
    14581 cgtggtttgc tgcccctgtg ccagttgctc aacgaaaaat gagtagcttc ccgcctgcgc
    14641 ctgggaattg gcggaaacgg tcgccgagtc attattgctg gtggcggcaa acgtcaccgg
    14701 gccgtcggta tcgctgttca gcgcgtcaac ggcgctctga aaatcgctca acgctgaact
    14761 gagcgaatcc aggccgctgc tttcagcatc cagctctgtt tgcttctcct gcaagtttgc
    14821 cgcctgagtg gcgacatccg catacgcgat ttcttgcgca atggttcttg ggttgatcat
    14881 tgatgactcc ggataacgat gtcaggttat tggcaaaagg cgtgccaata ttttttatgt
    14941 gaataatcaa tagattaaaa aacaggcgga aatggcgttt ccggtctggc ggaaatggcg
    15001 tttccggtct ggcggaaatg gcgtttccgg tctggcggaa atggcgtttc cggtctggcg
    15061 gaaaaaagcc tgccgcagaa agcacaacac cgcccgcagg cggtgttgtt cgtagtccga
    15121 ataaggcgtt tacgccgcat ccggcaagtc cgccattact gcagcaggct ggaaaccatg
    15181 ctggacatgc tgttggactg tttcagcatg gtgatgccag tctgcatcag cacctgctgt
    15241 ttggtcatat tggacgcttc cgtcgcgtag tcggtatcca taatgttacc gatagcgact
    15301 tcggtgttgt cctgcatact ggtcaggttg ttggcggtgt cgttcagacg gttgatagac
    15361 gcacccagct tggactggat ctgcgaaacg tcatccatcg cggtagagat gctggtgatc
    15421 atctggttag ccgagccaga cgcggtcagc tcagtaccgc cagaaacacc tgcagtaccc
    15481 gtggtgtctg cctgatccgc gctaaaggag ttgctgatag cagaaagatc ggtcaccagc
    15541 gtgttcagtt ggctggagat gttcacggtc atggtgtcgg aagattccgc gcccacctgg
    15601 aaagttaccg cgctctggaa cagaccgtca gtaccggtgt tgctggtacc ggaaacaccg
    15661 aacaggttgg tgccgccgta agtggtgttt tgcagcatgt cagacatctg ctgacccagt
    15721 tcgtcgtatt cgtcctgcat cgcctgcaga tcgtcgtcgc tgtaggtgcc gttcgctgcc
    15781 tgagttgaca ggtctttcat acgacccagc acatcagaca tttcatcgaa catactatcg
    15841 gcggtttgca gcatcgcggt tgcgtcattg atgttgctca gcgccacgcc cataccgctg
    15901 gactgtgctg tcagacggtt agcaatttgt ttgcctgcgg cgtcatcggc agaagagtta
    15961 atccggttac ctgtcgccag gcgttccata gatgtagaca aagaagagct gcttttgctg
    16021 attgcattaa cggcggccat tgaggcgtta ttggtgttaa tagataacat gatactgctc
    16081 cttgggatta atcgttttcg tttcgatacc ctgtaaaaac gacacctaag gagcaaactt
    16141 aaaaccgccg ttaaaaaaat ttcactgcga tttatctgat gccactttta gctaaaaaaa
    16201 gcagagttta aattcagata aaaagattat tagcaatatc tgccacatgg ccaggtatca
    16261 aaatatgtct ttatcaaacg cttgatatag ttaagattac catcaattaa cgcgttttct
    16321 tctcaatgta ctgatgccaa aatttacatt gcgttgcaaa tagtacacat ttggctattg
    16381 taccagtcct gtcatttcgt tacatttcca cgccatatag tgattaagaa taatcctatt
    16441 accataaaat attagttatc acgcagaaaa ataatcttct cagggacgta taagactgtg
    16501 ctttatgcaa agcactatca ggaaacattt atcccttgct aaggtaaact caactatgag
    16561 tattattatc aacaactggc ggatggaccc gtcgcttaat gccttaatac attgcgaaac
    16621 gggtgaaaca cgtcgtctcg gcgaatacca ttttattttg ctggaaacat tagcaaaaaa
    16681 tgccgatgtg gttttatcgc gatcttattt atgtgccgaa gtatggaaga accgcattgt
    16741 cggcggaaac agcctgccga cggcgatcca tgcgttgcgc gttgctatcg atgatgatgg
    16801 caaacaacag aacattatta aaacaattcc gaagaaaggt tacttatgca ataaagagta
    16861 tgtgtctttg ccggactcat cgcctgcaaa aaaactgatt attaccgacc agatacagga
    16921 aacagtacca gaagagatat cttctacaac accgcctgtg cctgttagaa aaaaacataa
    16981 agtaataatg gggctagcgt tagccgcggc tgttatattt attggctcga ccgtggggta
    17041 ttcacacttg aaaagtacac ctgatgcccc gcaactggta aaagagtcaa ttaacagtcc
    17101 aagaataaaa atatttcacc ttagttctgg aaaagaaaat aattccgtac cgttactgtc
    17161 acaaaccctc gcacccggaa aagacaaact cgataattta ttgtcagcgc ataatatgac
    17221 aatgacaacc tattataaat atgttcgtaa ccgactggaa agtgatattg ttctgcgcaa
    17281 ccagtgcaat ggtagctggc aattaacttt taatgtggaa ggctggcaga acagcgatat
    17341 taatagcgcg atgtatcaga atttagagaa gttgttaaat actttgcaga aatgctgatg
    17401 agtgccaata tatgccggat gcgacgctaa acgcgtctta tccggctgat aattacccca
    17461 ataaattcct caccgtctgg ctggaaattc gcgcctgacc attcaccgcc tgcaataaaa
    17521 cctgatagtt atgattcgcg ctatccagca cgccgccgag gttttccgac gcctgcaccg
    17581 cgttttccgt ttgcgggaag cgcgacatgc cgtcgatcaa ctgccgcgcc ttttcctgct
    17641 gtaccgccat ctgcgcacgc tgctgattaa tgctttctaa cgtctgagaa attcccgcac
    17701 cgccctgttg caggctttga gctaaacggt ctgcctcaga cggctcggca aaagttttca
    17761 gcggcataaa cgccgactta tcgccatcgg tgcgcacgct taaggtacgt tcgatatggg
    17821 gccactgttt ttcggtggtg gagaaactga tgccgtccgc ttgctgatgt agctgcacgc
    17881 ccacgcggcg cagggcgttg gtcagccgcg tctgatactg cgcggcgctg tcatcctcag
    17941 acagcatcac cgccgaaagc tgagtctgcc gcccgtcaga gacgctgaac atgcgcgttc
    18001 ccggcgtggg attgtgtacc agattcgcct gatccggcga gtgaaaggtc acccgcgctt
    18061 cgccctgtaa caccggctgc aactggcggt ccaccgcacc gccggaaagc gcggtgcgtt
    18121 tgtccagcat ctgcatcagc gccgtattct gcgcctgccc gcctttacgc tggctgtgac
    18181 gatagtcgag caactgctgc tccagttgcc cgagataatg atccgcctgc tgcaacgtgg
    18241 tgagctggtc gttaagctgc acgctgtaac gctgcgggcg agtggtaatc agcggcgagg
    18301 ccggatattc actcgccgtc gccgggagtt tttgccgcac cgcctgagtg ggcgcaacgg
    18361 ttgacgacga taccgcagcg ctatgcgcgc cgccggtggc gagtgatgaa gatgtaagtc
    18421 cgacctgcat actgttcctg aaagagttag agaacgctga acaaattcaa ctcactcact
    18481 ttcgagtaag tcttgagggt aacttccatt gccacttcca gtccggtaaa ggtgatcgac
    18541 gccgggccgt aatcgagatc ctgtaatgaa ccaatcagct catcattaga ggtggagata
    18601 tcggtctgcg catcgctcag catcgacatg gtattttgcg tctcgccgag tgacgcaatc
    18661 gaggcgttga ggtcgtccga ggcggtgtcc accacatcaa cggcattctg aatatcgctc
    18721 tgcacctgcg gatctgccgg gtcgacgtcc gggttttgca actcctggga tagggaattc
    18781 agggtgttga gcacgtctaa attgctgcca aagaaatcac tcgccgccac gttggtgtcc
    18841 acttccacgc cgttcgacac cgtggtctgg cggtggtcgc tgttaccctg atagctgtag
    18901 ctgtcggtga cgccatcgcc gtcatcgtcc actgccacaa tcggcggctg atcgttgatg
    18961 gtgccgccaa acacgtagtg cccgtcttcg tcctgataat tgagcgccgc caccattgat
    19021 tcggtgagcg actcaatatc ctgcccgagg cttgcgagtg aatcggcggt attggtgcca
    19081 ttcgctgctt ccagcaggtc gtcgcgcacc gccagcaggc tgttgttgac gccatcaagg
    19141 atcgactcct gctggcttaa gcccgccgac gcggagtcga tattgctctg atactgctca
    19201 atcgccgact gctggcggtt taactgggtg attcgcgtgg cggcgatcgg gtcatccgac
    19261 ggctgcaaaa tctgcttgcc ggttgccatc tgctcgacaa catgcgccag atcgccagag
    19321 agattattaa aactttgcgt catcgagacg taggtttgtt gggtagttac tcgcattttc
    19381 gcgctcccgc ttagctgcac atttccagca ccgaatcgaa aatctcggcc ccggcggaaa
    19441 tgactttcag attggcttca taaatttgtt gataggtgat caggttcacc gcctcttcgt
    19501 ccatgctgac gccgctgacg ctgctctgct ggttttgcgc tgcggaatag acattggacg
    19561 cggcatcgac ttccgtctgg ttttgctgac tgtaaatgcc gatattgctg atgattgacg
    19621 agcacgcctg cccgaccgtc acgctgccga ggttggctat ttccagcggt tcggtggaga
    19681 tattgatcag cgcctgcagg ttgtcgctgt tgccgctttc gtccggtgaa ctggaaaacg
    19741 ccagctcatc ggcggtgata ttcgggttaa cggtcagcgg gccatcggca ttgctggcgt
    19801 cataaataaa cagcggctcg ccggggttac cgttgagatc gtagccctgc gccagctggt
    19861 tgttgaccgc atcggcgaac tgcgacgcca tgctgttgat ggtgtcggtc agcggcgtca
    19921 gcacatcgtt ttgataatca aacagtgcgc ctaatgaacc gccggtgtcg gtggtcatcg
    19981 tcgaggtggt gccagcgaag gtcagcgaca tggtcgacgt gccatcggcg ttggtttcca
    20041 gcgcgatggt cgaactttgc tgcccactca ccagcggctg accgtttttc agggtaacgt
    20101 tgtagttgcc ctagtcgtcg atattgacct gcacatccat catcccgctc agttcttcca
    20161 ccatctggtc gcgggcgtcg tacagcgcgg aggcgttatc gccattggct tcagcctgag
    20221 cgatttgctg gttatagctg gcgatgccgc tggtgagcgt attgatttgc gacaccatcg
    20281 cctgctgctg gctgatgatt tccgtgcttt gcgagtcgat gtaatccagc gtgttatcaa
    20341 tacgcaacga cagcgccccg gcttcactga tcacctgctc gcgcagggcg gaatcatcgg
    20401 ggctggtggt tgcttcgtta agggcggcga agaagttatc gaagccgccg ctcaggctgc
    20461 tgttatcgtc gctcagtacc gcttccagtt ggctgagata cccctgctgg gtgctgtaat
    20521 agccgtaatc gctggcggca taccacacct gattcacctg atactggttg gagacgcggc
    20581 gaatgctgtc gacctgtacg ccgttaccgg cgctgttcgg cgaaccgccg ctggcaccga
    20641 tggtgctgat ctccgccacc tgacgggtgt agcccgtggt catggcgttg gcggtgtttt
    20701 gcgccgtgac gttaagctcc acctgcgcgg ttgatgcacc gctgtagccg atgttaatca
    20761 tgtccatgtt ggatccttac cggtaaatcg tgccggttaa aagcggcgta aagttgggga
    20821 ttcttaagtt ccgtaaaccg ggtgccgaac gccggatgcg gcgtggacgc cttatccggc
    20881 ctacatacgg gcgactcgta gatactgata agacgcgaca gcgtcgcatc gtgcaccgaa
    20941 cgccgaagct catttatgct tcggcgacaa ctgcttcaca atcatcgccg caatgccaat
    21001 cccgtgctgc tgcgccaggg tgttacacaa ctcatcgtca taaaacccct gcatcatgcg
    21061 caccgaatcg ctgttaaacg ggttatcttt tgaatcaaac aactcgttgg ttttccgcat
    21121 ctctttcagc atgttgcgta aaaaaatcgc ctcaaactgt tcggcggcct gcttgatatc
    21181 attggcctga accttcggcc ccattaacgc gtcgctgccg tcaatcccgc cgctggcgtt
    21241 cactttcatc agatcacctc caggtcggca tccagcgcgc ccgcctcatg cagcgcctgc
    21301 aaaatcgaca taatgtcgtc cggcgtcgcg cctaaactgt tcagcgcatt gacgagcgtt
    21361 ttcaggctgc tcgactccgg caaactcacc acgcccggac gggcatgatt caccgcgata
    21421 ttgctctgcg gcgtaaccgc cgtgcgccca cccgcaaagg cattcggctg gctgacgttg
    21481 ctggtttcgt taatcgaaac ggtcaggctg ccgtgcgaca ccgccgccgc gtgtagcgca
    21541 acgccatcgc ccattaccac cgtgccggta cgcgagttaa acaccacgcg agcgcggatt
    21601 ttctccgcct gcacctgcac atcgtccagt tgcgacataa atgccacccg cgcgccgggg
    21661 ctggtgggcg cgcgcacggt aacgttggtg gaactctgcg cggtggcaat accgccaaac
    21721 gaggcgttga tcgccgccgc aatgttgttg gcgtctttaa acgacgggcg tttcaggttg
    21781 agggtgatgg tatcgcccat ctgaaaatcg ctggggatct cgcgctccac cgttgcgccg
    21841 ttaggaatta acccggcggt tggcgtatta accgttacgc tggaaccgct ggccccggtg
    21901 gcgttcatcc cgcccaccac cacgctgccc tgtgccaggg cgtaaacttc cccatcagcg
    21961 ccgtgcagtt gggtcagcag caaagtgccg ccgcgaatac ttttggcatc gccaatcgac
    22021 gaaacggtga catcaatggt ctgcccgcgc gagtacatcg gcggcagcgt ggcgctcacc
    22081 gccaccgcgg cgacgttttt caccttcggg tcgattttat tcggcagttg cacgccaaac
    22141 tggcgcagca tattggtgat ggtctggttg gtgaatttga cctggttttt atcaccggtg
    22201 ccgtcgaggc cgaccaccag gctatagccc accagttggt tttcgcgcac gccctgtacg
    22261 ttgaccagcg actccagcga ttgcgccagc gccacaccag gcagcgccag gctcacggcg
    22321 ataactgcgg cctttatcca gttttgcatt gcactctccg gcattaaatc gggaacagag
    22381 gatggttaaa cagccgcgtc agccagcctg cggcgttggc gtcgcttaac gcgccgcgtc
    22441 cggcatagga gatgcgggca tcggcgatcc gctgggagga aaccgagttg tcgttctgga
    22501 tgtcgtcggc gcgcaccagg ccgcttaagc ggatgtactc atcgccctgg ttgagggtga
    22561 gccatttctc gccgcgaatt tccaggatgc cgttgggctg caccgcatgg acggaaacgg
    22621 tgatctcccc gcgcaggctg ttctgctgct gggaagtggc gctgccgtca aagtcgcggt
    22681 tggcgtcgat agagccggaa agcttatctt tagtgtgact aaaaatggtc ggtgcgccga
    22741 tatcgacggt gttgcttttg ccgaagttgg ttttcgcctg tttgctcgac tgggtggatt
    22801 cttcgaggat caccgtcagg atatcgccca cgcgataagc ccgccgatcg gcggtcagcg
    22861 accagttgta gccagtttca aatacaccgc cagcacgccc gtctgccgga ggcggtgctt
    22921 cggttttcgg tggcgcaaag tatgcgtcgt cttttttgac cagaatggcc tgcgattcac
    22981 acccgctaag caggggaagc agcgccacca gccagagata atttttcacg ttctgcctgt
    23041 ttaaatacag tgagttatgc gcttttgtag gtcggataag acgcgtcagc gtcgcatccg
    23101 acatttcggc cttacagcgt ctgactgatg tactgcaaca tatcgtccgc agccgacacc
    23161 attttggcgt tcatctcgta agcccgttgc actgtaatca tcgccaccat ttcgttgacg
    23221 atatctacgt tcgagccttc gagggcattg tcctgcaaag tgccaagccc atcttcgccc
    23281 ggcacacctt cggtcggctg accgctggcg gcggtttcca gatacaggtt gtcaccctca
    23341 gcggaaagcc cagccgggtt agcgaagtta accagcgtca gttgccctaa ctcggtcacg
    23401 tcgctgtcgc caggaagaat cgccgtcacc gtgccgtcct caccaaacgc aacattcgtc
    23461 gcccccgccg gaacgtcgat ttccggctgc aatggcaagc cctgtgagtt ggtcagtacg
    23521 ccatcggcgt tcacctgcaa attgccgtcg cgggtatagg cgatatcgcc gttggccttt
    23581 tgcacctgca aaaatccctg ccccataatg gcgacattca tcgcgttgtc ggtggtttcg
    23641 acgttgcctt cggtgaaggt tttctgggtg ccgacaatgc gcacgccgct accgaattgc
    23701 agcccggtcg gcatgatgtt gttctggtcg agcatcgcgc ccggcgcttc cagggtctgg
    23761 tagaacagat cctgaaacat cacccggtca cgcttgaagc cggtggtgtt aacgttggcg
    23821 atgttgttgg caatcgcgct catttcagca tcctgcgccg agaggccggt tttgctgatc
    23881 cataatgctg cgttcatggt ggtaaattcc gtgttatttt tgggccgcgg ttgccggatg
    23941 cggcgtgaac gccttatccg gcctacgatg tgcggttacg agccgcgcaa taagcggttg
    24001 cccgcgtcgc tgatatcttc ggcggttttc atcatcttga tctgtgcttc gaactggcgg
    24061 ttcatggcga tggacgacat catttcgctt accgccgaga cgttgctgcc ctcgagaaaa
    24121 ccgccgctga ctttgatgtt ttcatcgcgc tgggccggaa cgccgtcggc agtcaccagc
    24181 atcccttcag catttttctc caggttagcc accgggatat ccaccagctt caggcgatcg
    24241 atatccatcg tcgcggtcac atcgccgtca tcgggcgtca ccgacagcgt gccgtcgctg
    24301 ccaaacgaag caatggcgtt cggcggcaga atgatcggcc cgttatcccc cagcaccaga
    24361 ttgccgtcga tggtcagttg cccctggtcg tcctgctgaa tgttgccgtt gcgggtatag
    24421 acttcgttac cgtttttatc ctgcaccgca atgtaacccg cgccctgaat cgccacgtcc
    24481 agcgggcgtt cggttttctc cgcaacaccc gtgctgtcgt tcacgccgct ggccccttcc
    24541 tgcgccatat agcgcgtgtc gaagccgccg cctttcacct tgtcgttggt tgccattgcc
    24601 atatcggcgc gaaaaccatt ggtgttgacg ttcgccaggt tgttggcgct aatttgctgc
    24661 tcatacaggg tctgagaagc accgctcagg gcggtataaa tcaggcggtc cattacattg
    24721 cctggaacag cgcgtcatcg agctgcgtgc tggtggcgat aactttggtg ttggcctgat
    24781 agttacgctg ggcggtcatc aggttgacca gttcgctggt gatatcgacg ttagagcttt
    24841 cgagcacgcc cgacgacagc gtaccgcagg tgccggagcc gggaacgcca atcagcgggg
    24901 tgccggattc gcccgtttgt acccatgcgg tgccgctcac tgcctccagg ccgttttcat
    24961 tcgggaaagt cgccagcacc acctgcccct gcaacatgcg ctcgccgttg ctgtaggtgg
    25021 cgtaaacttt gccatcgtca tcaacctgaa caccgttttg cgtggcggag gcgtagccgt
    25081 tggcggcgtt ggtggtgacc gaaaattcag agccgtattg cgtacaggtg gagtaatcga
    25141 cggttaagtc gacgggcgcg gcggcgtcgg tttgaaattc aatggtctgc ggcgtggttg
    25201 gagaggtcag cttcccggtg ttcggatcga aggttaacgt cgtcgccgga acgccggtct
    25261 gctgctgacc gtcgaaggtg tactgcactt cccaggtgtt gtcgctggtt ttggtgaagt
    25321 actggcatac cgaatgttcg ttgcctaatg agtcatacac cgtggtggtg tagctgtcgg
    25381 tgtacgaact gctgttagtc gcgtcgaacg gaacggtggt gcgatcgata gcttcatcgc
    25441 tggcgttgaa gttagcggta aaggtcaggc tgctgctggc ctgcgccggg atattgccgg
    25501 ttttgatctg gatatcggtg accgtaccgg tttgcagagt gccagtgtca tcgaccggat
    25561 agccctgcaa ataagcgccc gaggcgttga cgatatagcc gtttttgtcg gtttcaaacg
    25621 atccggcgcg ggtataggag atattgcccg cgctgtcaca catgacaaaa aatccgtcgt
    25681 cgttaatcgc taaatcgagc gcattgccgg tggagaccag cgaaccgcca cgcgaaatac
    25741 tttgcgcggt gcccgccacg ctgacgccca tcgcctggct tccggcatac atggcggaaa
    25801 actgggtggt catcgactta tagcccaccg ttccggcgtt agcgatgttg ttactgatcc
    25861 cgtccagctg ttcgttaacg gcattcagcc ccgttgcggc aatttcataa ctcattgtgt
    25921 cttcctgtta attgattctt acgccacatc actgtcggca tcgggtacgc caaactggct
    25981 aatcatggtg aacggcactt cgcccacgcc tgcaacgtta agcaccgggg aactgccgtc
    26041 gagcgggatg cgcacatctt ccacttcgcc ggagacttca atcggcacct cttcttcccc
    26101 ggtattggtc accaccgaga cgtcgtaatc gcccggcggg atgccgtaat ccgcgggatt
    26161 caccgagaaa tcaacctggc ctggtccgac ggacgaactg ccttctggga tcagcgagac
    26221 ggtgtagtca tcgcctgcgg catcggtaaa gtggagatcg gcggtggtac aggcgtcgtc
    26281 cagcgtagcg cgaccgttaa tggtctggtc gctcacctgc aaggcggtgg tctgcaccat
    26341 aatgtcgtcg cccaccaggt taccgagcgc catcacctga atgttgctca tcagcaccgc
    26401 gttggtgttg gcctgcacgc tcatctcttc catcatcgcc acctgcgcca tcgacgacag
    26461 ctggtcgatg tattcggtag cgtcggtcgg gtcggtcggg tcctggtttt ggatctcggc
    26521 gacaaacagc gtcaggaagg tatcgacagg cgagctatcg ctgctgcttt gcgtgtcgct
    26581 ggccgccaca ttattgcttt ccgccgtggc ggtcggctgc ggcgattgcg cattcagcgc
    26641 cagggtattc atcaggcttc tcccagtttc agcaaacttt gctgcatact tttcacgttg
    26701 ttgagcacat cgacgttggt ttcgaaatcg cgcgaggccg acatcatgtc cgccatctgc
    26761 tccaccacgt tgacgtcggg ataccagacg tcaccgttgg cgtcggcgag cggagaatgc
    26821 ggttcatagc gtttcaccgc cccgccggtt tgcagcacgt cctgtacctg cacgctggca
    26881 ccgtcaatgg catgacgatg tgtacccgcc agcaggctgt ggtgatacac cgcggcaaac
    26941 accgggctgc gcgctttata cgcctgcgcc tcgctgctgg cgggcgcatt ggcattcgcc
    27001 aggttactgg caacggtatt cagacgtagc gtttgcgccg tcatcgccga gccggaaatc
    27061 tgatagatat cagtaaatga catgcttatt tcccttcaat ggcctctctg atgccgctga
    27121 tttgcagatt cagaaaggtc aaactgcttt gataatccat actattttgt gaaaaccgcg
    27181 cctgctcgct gttcagttcc acggtgttgc catcttccga cggctgcatc ggcacgcggt
    27241 actggacatc caccgccgcg ttatttgccc gttgcatctc cctggcaaaa tcaatatctt
    27301 tagccttgaa atttggcgta tcgacgttcg ccagattcgc agtcagtagc tcggtcctct
    27361 caagacgtaa cttcactgcc tgcggatgta ctcccaatgc ctgctgaaaa ctgatcccca
    27421 tttcctgcat cactcctgct tcaatccgca tacgctaagc aatcgccatg ccatttttta
    27481 agtcactgat gagaaagggt ttatgagttt tcaattttgc aggcggaagt tttacttcct
    27541 cctggcgctg gccttaccgg gctacgccgc cgtccatccc gtgcaacaca gcgcgcggga
    27601 acaagtcaac gcccaggtgc tgaatgccgc gagccaaaaa attgaatcgc tggcgcagca
    27661 gcggcagtgg catgactatc gctacacctt taagatctac attccctcgc agattgcgac
    27721 cgccgcgccc tgtactaaaa cacccggcgt cacgctgact tcgcctgcgg aaatcgccct
    27781 caaccggatg aacttcaccg ttagttgccc gcaaagctgg cagatgaacg tggcggtgcg
    27841 cccggatgta ctggtgccgg tggtaatgac gaagtcactg gttgcccgcg atatgccgct
    27901 caccgcgaat gatgtggagc ttaagcccta caacgtcagc gcccagcgcc gcgatgtgtt
    27961 gatggagctt gatgacgcga ttggtttaag cagtaagcac gcattgcaac caggcagacc
    28021 catcaccaaa gaagaactgg tgtctccggt gctggtcggg cgcgatcagc cggtgatgat
    28081 tgtttaccag agcgcgggga tcaccgcctc gatgcccggc gtggcgctga aaaatggtcg
    28141 taagggcgag atggtgaaag tgcgcaatgc cagcagccag cgggtcatca gcgcgatggt
    28201 ggcggaaagc ggcgtggtga caacggtaag cgcggagtaa tttgcgcagg cgattaaagt
    28261 tttgccgcct gttaaccgct ttaccgcagt aacccaccca aataccgaga cgaggttgcg
    28321 atgaaaatca ccccgactat gcctggcaat cgcccgacag ccacaacggc aggctccagc
    28381 cagccgcgca aaaccacggt gaccaccacc acgacgctga gcgccgacga cattactcag
    28441 gcgggattgc agtctgccca gcaaacgctg aacgaccagc aagagagcga tatcgacagc
    28501 aacaaagtgg cgcaagtgca ggcgatgctg gcgtccggca gcccgcaagt ggacactgaa
    28561 cagttagcca gcgacatgct gagttttttc cagaactaag aggccgatga agtgagtatc
    28621 cggttacaac aggtgaaggc gttgttgcag ggcattcgtg acgatgacgc gctgtatgat
    28681 tccctgcgcg aacttctcca gcgtcagcgg atctgcatga ttcgccgcgc cagcgaagag
    28741 ttgctggcgg taaacgatga gataacccat cactatgagc agttgcatgg acatagccat
    28801 cagcggcata gcctgctgaa aatgctcggt gttagcgtaa atcgtgacgg tctggcgcag
    28861 gttttcacct ggcttccggc tgttcaaaaa gcggctgcac aacagttgtg gcaacgcctt
    28921 gagcaaaaag cggaacgctg caaagcctac aacgacaaaa acggcgagct gttgattcgc
    28981 cagtatgaat ttattcagtc gtttttaggc agcgaagcag attttctcta tcaggaataa
    29041 cgcgaatacc ttccccatag cggaaatgga ttttccgcct gatttccaat cccttgtttt
    29101 taattacaaa aatccccggc acgcttattg catatcctgc acagcattaa agactggaag
    29161 ccacctatga gttttattga atgttacgaa ccggaatacg tgcgcaaatt tttagcgcgt
    29221 caccccggtt cgcctttata cgtccgcaag gtgtggaaaa acgagattcg cctgagcctg
    29281 tcattgaccg atattgccag ctgcgaagcg gtgctcaacg atgtcagggc ctttgattta
    29341 cagctcacct accgccctgt ggaagataat tccgcagagc tttccgcgcg cgatgcgata
    29401 gtaaaccagg tgattttgag taccctcacg ctccgtgatt taaccccgga gctttccctg
    29461 tatgctgtcg ggattttgct ctcctgcgcc agcaaaatgc cgggcagaga cggagacatc
    29521 ctggcccgtt tgacgacgct gccacaggcg ctggccgatc atgcgaaaaa aggtacgcta
    29581 caggcacagt acgcccagct cccgcccgtg ccacagctgg cgcgccagtt gatgacgctg
    29641 ctcggcggtt gtgcgtttga ctggtcgatt ttgccggaaa gcccgcgcaa agcctcgctg
    29701 ccgttgcagg ttactttact gacgctgcat gacgccaaca gcgaagccct gttacaacag
    29761 cagcttcaga cgcagtggca aaccacctgg caacagcatt ttgccaccgc gccctggatg
    29821 atgcgtaact ggctgattta ccgcgtttac cacgatatga ttgggcagac tgacggggcg
    29881 gattatttcc cgctggtgtg cgatttttat ctcctccgca cgttaatcag tttatggacg
    29941 ctggatggtt ctccgctgcg gcaggaggat attttcgccc tctttgccat gtttgaacgc
    30001 tggcgagcca gtgaaaatgc gctgttggtt cgccagcaaa ttcagtcgtt atgtgccgcc
    30061 gacccgctgc tttcagcgtt ttcgctgcta acgtgagtcg ctctgcgcgc gctttgcccc
    30121 tcaccctaac cctctcccca aaggggcgag gggaccgatc gagcacggtg ttaaaatcgg
    30181 cgctgatttt ctccctctcc ctgtgggaga gggtcggggt gagggcatca gcgcgcacgt
    30241 tcatcacatt cacacattaa ctcacccaaa caccatgcca acaccaacca tcatcaaaac
    30301 actcgccacc caccgcgaaa aaccgtttgt cagcgtcgtc acgccaacct ggaaccgcgg
    30361 cgcattcctg ccgtacctgc tctatatgta ccgctatcag gattacccgg cagaccgccg
    30421 cgaactcatc atcctcgacg attccccaca aagccatcaa cacatcatcg accgcctgac
    30481 caatggcacg ccggaagcct ttaacatccg ctacatccat cacccggaaa aactgccgct
    30541 gggcaaaaaa cgcaacatgc tcaacgaact ggcgcgcggc gaatacatcc tctgcatgga
    30601 tgatgacgac tactatccgg cagataaaat ctcttacacc atcgctatga tgcaaaaaca
    30661 ccgggcgctc atctccggtt ccgaccagat ccccatctgg tacagccata tcaaccgtat
    30721 ctttcaaagc cgcagctttg gcccacataa tatcctcaac ggcacctttt gctatcaccg
    30781 taactacctg aaaaagcacc gttacgacga tgattgcaat ttaggcgaag agaaaagttt
    30841 caccgacaat ttcagcgtta acccgctgca actgcccggc gagcgcacga ttttgtgtat
    30901 ctcgcatagc cacaacacct ttgataaaga ctttattatc ggggccagca cgccggtaaa
    30961 cacggcgctt acggatatcg tccgcgatcc gctgctgcgc aatgcttatc tgagtctgca
    31021 taacgccact catcaccagg cgatcaacca tcaggcgatc gatcaggtgg tcgtcgtgaa
    31081 tctggataaa cgcccggatc gcttacaaca gatccgtgaa gaactggcat tgctgcatat
    31141 tccacccgag aagatcaccc gccttgccgc cagtgaagat gaaaacggtc aacggggccg
    31201 ccgtcagtcg cacttgcagg cgctgcgtct ggcgcaacag cacggctggc aaaactatct
    31261 gttactggaa gatgacgcgg ttattctcaa gcaggagaaa catattcagg tactgaacac
    31321 gctgctggcg agcctggcga aaattccctg gcaggtgatg atcctcggcg gcgaaatttc
    31381 gcaaggaacg atgctaaaaa gtttgcctgg gctggtgcac gcgcgggact gccgcaaagt
    31441 gtgcgcttat ctggtgaaca gccgctacta cccgcaactg gcgcagcaga tgagcaacga
    31501 tgagcactcg ctggaagact gctggcaacc gctgctacgc gccgacaaat ggctggcctg
    31561 ttacccgagc ctttgttatc agcgaccggg ctttagcgat atccagaaaa aaacgactga
    31621 taatattgct cgctatttca ataagttacc cgtagccacc aagccatccg cgttacccat
    31681 cgccgacacc atcggctttt ttatggaaac ctcattccac tacgcgctgt accgcccaat
    31741 catcaccgcc ctacaggcgc aagggctgag ctgcacgctg gtgatcaacg accgcgtgtt
    31801 taaaccgttt ctggatgaga tgctggaaac actgaaaaac atcgacgatc cgcagcttaa
    31861 aggtatgcgt ctgtcagaaa tgcagacaca tggtcagcgg gtgaaatgcc tggtcagccc
    31921 ctaccacacg cctgcgctca atggcctggc ggcggtgaac atccgcgcta tgtacggctt
    31981 agccaaagag acgtggaacc acgccgactg gaatcgtttt taccaaagca ttttgtgtta
    32041 tagccactac agtcagcagg cgcttgcgca tttcggcagc gcaaaagtgg tcggcaaccc
    32101 gcgcttcgac gcctggcata acggcacgtt cgatcgcgcg ttgccggaaa acatccaatc
    32161 agattaccgt aaacctacgg tgctctatgc cccaacgttt ggcgcattaa gctccctgcc
    32221 ccactgggct gaaaaattgg gccgcttgag cggtgatgtg aacctgattt gcaaactgca
    32281 ccatggcacc tgctcacgcc cggaagaagc agcatcgctg gccctggtgc gcaggcattt
    32341 aaaacagcgc accgactccg cccgccacac gctggcgctg ctggcaaaag ccgattacgt
    32401 gctgaccgat aacagcgggt ttattttcga tgctatccac gttgataagc gggtgatcct
    32461 gctcgacttt ccgggcatga atgatttgct cgacggtgag aaaagttatt ccacggcaga
    32521 gagcgccgac cagcgaatcc gcgaaatatt gccggttgct catgatgttg cagaactgcg
    32581 ttatttgttg tcagaggcgt ttgactgggg gtcggtgcag gcgcggctta cggagattcg
    32641 ccatcactat tgcgatgcgt ttatggatgg caaggcggga gaacgggcgg cgattgtgat
    32701 tgtggaggcg ctggggtaaa ccgcgcaact gcctgatgcg cttcgcttat caggcctacg
    32761 gatcgatcac gtcctgtagg ccggataagg cgtttacgcc gcatccggca agaaaataca
    32821 tctctgtttt agtactttat aaaagccctc taatcacctc aataatcccc gtcgtcgaca
    32881 ccgacggcgt gcgggggaaa tagaccacct cacaaatata cgcaaagctg tcaaacttcc
    32941 ctgcccagtc atcccccatc actaaaatat ccgcactgta cccgcgcaaa tactccgctt
    33001 tctgctctag cgattcttcc agaaatacgc catccacgca cgccagcccg gcgatgatcg
    33061 ccatgcggtc atcctgatgg taaaccggtg ccctgccctt tttggcgatg ttcagggcgt
    33121 cggacgatac gccgaccagc agccgctcgc ccaatgcgcg ggcgcgttgt aaaaggcgca
    33181 gatgaccgac atgaaacaca tcgaaggtgc cgaaggtgat gaccgttttc atggctactc
    33241 cgctaatttg tgccgcagcc agcactgcac ggcgatgtcg tccaccgctt tttgcgcttt
    33301 cgccgtggcg ctttcttgtt gctcgcggcg tttgtcgtcc agcgtcaggg cgactacttt
    33361 ctcttcgcac gccgccgccg tgaggttttt ctgcattcgc gtggctttga tattggccag
    33421 cgttttttcc tgctcctgcc aggcgattac cttgcgcagg gtgcctttgt agccgctgac
    33481 gtttttcagc gattccaccg acaccgccgc acccgcagat gttttctcga caaggtagcc
    33541 taacgccttg atattgttgt cataacccgc gcaaatctgt ttttgctttg ccagctcgac
    33601 ggttttgtct ttgaccgatt tatcccgcag gcgctgcaac tgcgtcaggg tgtcgatgat
    33661 ttgtcgcata gttactgttc tgcctttttg acaccaggaa agagaatttg caggcggctt
    33721 tgcaccagtt cgagggaggc gggttcgcgc atctcctggc gcaaaaatcg ctcgatggcc
    33781 gggaacattt ttaccgcttt gtcgacgctg gcgtcggcac ctgccacata accgccgagc
    33841 gggatcagag gtttgatctc catataggcg gcatagtttt gctttaacgc gcgggcggat
    33901 tgctgatgtt ccaggctggt gacctggttc atgcagcggc tgatcgactg gccgatatca
    33961 atggccggat agtgccccgc ttccgccagt ttgcgggtta atacgatatg cccgtccagc
    34021 accgcgcgcg cgcagtcgac aatcggatct tgctggtcgt caccttccgc cagcacggtg
    34081 tagatggcgg tcatcgatcc cgcgctttcg ctgttgcctg cgctttcgac cagtttgggg
    34141 atcatgccga atgccgatgg cggataacct ttggtggcgg gcggttcgcc gagcgagagg
    34201 gcgatttccc gctgcgccat cgcgtagcgg gtcaggaaat ccaccagcag taagacgtga
    34261 tgcccgcggt cgcgaaacca ggcggcgatg gagtggcaca gttcggtggc tttcagacgc
    34321 atcagcggcg attcatcggc aggcgcgacg acgacgatag actttgccag cccgtccgcg
    34381 cccagcgagt ggtcgataaa ctctttcact tcgcgcccgc gttccccaat cagcccaacc
    34441 accacgatat cggctttggt ctggcgggtg atcatcccca gcagcacgct tttcccgacg
    34501 ccgctgcccg ccattagtcc gacgcgctgg cctttgccga tggtcagcag gccgttgatc
    34561 gctttcacgc ccacgtccag cggttcgtct accgaacgac gagttaaggg attaaccgag
    34621 ggcggcaacg gcggcagaag gtcgttgccg ttcaggcggc ctttgccatc cagcggttcg
    34681 ccgaggccat tcaccacgcg gcctaaccag ctttcgccta tcaggatgtc gtgggctttt
    34741 tcttccggga agacgcgtgc gcctgccatc aggccgaccg gctgtttaaa cggcattaaa
    34801 taggtgatgt cgcgattaaa gcccacggcc tgcgcgtcga tcagcgtgtg attggcactt
    34861 tcgatgcggc acagctgccc ggtcattagc gggcagccga cactctccag caggatgccg
    34921 ttaaggcgca ccagccgccc ggcgacgcgg gccagcggga tcgactccag cgagcgcagg
    34981 gcgttatcga acgcagaaaa atcactcacc ctgcgactcc ggcgtgagtg tttctttcag
    35041 cgcggtcatg cattgttcaa ggcgatgctc gcagccgata tccagttcgg atttgtcggt
    35101 gatcacccgg cattcgcccg gtggcaaatc cggcgaaggc gtcagccccc attcgctgac
    35161 cttttccggc accgcgtcgc gcaggcggtt aaactcttcc gtgctcagca ttacttgcag
    35221 ggtttccggc atcgccggga aggccgccag cgcctcttcc accagcgcca gcaactgcgt
    35281 gggttgcagc gccagttcac aacggatcac ctggcgggtg actttttcca ccagttgcag
    35341 caaatcttcc cgctgtttac gttcgatatg agcgaggaag tcgttcactt taccggtgat
    35401 ggcttccagc ggctgcgccg cctcaacaaa ctgtttgcgc ccttcctgct gcccggcgag
    35461 gctgccttcg gtgtaaccct ggcgtcgccc ctcggcaaaa ccttgctgat gaccttcgct
    35521 aaagccctgc tcctggcctt cggtcattcc ttgagcaaac cctttttgca gcccttcctg
    35581 aaatccgtcc atcaactggc gctggtaatc ggcggcgcta atactggtag tgagcggttc
    35641 tccggtcacc gcacgctggc gcggcgggaa acggtgcagg cgatagcgcc cgcgcagtgt
    35701 ttcaatggtc atggttactc cgccgtctgt tccgcaaaga gctgtagctg aatttcgccc
    35761 gcctcttcca gttcacgggc gatgcccatg atttcgcggc ggatctgttc gatacggctg
    35821 accggcaccg gccccagacg cgcggtgatg gcttccagtt gctgcgcctg ccgttttggc
    35881 attaccgcgt aaatcgaacg gcgcagcagg gcttcggtgc ctttcagcgc caccgcccag
    35941 tcttccatcg gcacttcgtc aagcagacgt cgacgcacct cttcgttctg gcggctgagg
    36001 ataaagaagt cgtacatctc atcctgaagc tgctccagca cctcttcgtc gcgctcgcgc
    36061 atctggtcga ggatcacctg ctggttgccc tggaagcagt tgacgatatc cgccgcctgt
    36121 ttgatgcctt tcacttttga gccgtgttcc gaaagcaccg acaggccgcg ttcgatcagc
    36181 ctgtccagtt catccaccac gtcgcggttg acgtcgttga gtttcgccac ccggtagaga
    36241 atttcgtttt gcaccgattc gttgagatac gagagcaccg ctgccgagat ttccggcgtc
    36301 agaaacgcga ggaacaccgc ctgtaattgc agatgttctt cggagatcag catcgccagc
    36361 tggcgtggtt ccacccattg caggcgcgcc atccgcgagc ggatctcatc gccgtagatg
    36421 ccgttgatca cactgctggc gatctcggtg ccgagggctt tgttgagaat accctgcaac
    36481 atggatcgcg atgcgccgtt gatcccgctc tgttcgcgga actcatcaaa gaagttattg
    36541 atcactttgc gcgccatgct ggttttcacg ccggaaagcc gtgccatgtt ttcgctcagg
    36601 cgcaccactt cttcgcggct gagtttttgc atcaccgtcg ccgccgcctc ttcgcccaga
    36661 cacagtagca ggatcgccgc ctgttccagg tagctgttgc tcttatttgt tgtcaattcg
    36721 ctcattgctg gtgatccatt gtctgagaac ttcggctacg cgatcggtgt cgctcatggc
    36781 cagtttttgc aggaactcga gttttacttc caggccggaa ctctgagacg gcaggctgtc
    36841 atcgccgggc agcgacggca gatcgacgtt tttgcgctcc tcttccggca tggcaatcca
    36901 cggttcgctg acggcttccg attctggtgt gataatcggt gcacgtaccg ccgtcagccg
    36961 tttcattacc gggcgcacca cgaagaacag caacaacagc gccagcaggc cgcagccaat
    37021 caggcgcacc caggccagca cgctgtcatc tttccacagc ggaataattg gttcgaccgg
    37081 caccgcctgc ggcacaaagt taagcagtga aagcgtcagg ttatccccac gttgcgcgtc
    37141 aataccggcg gcattgttca gcaacgcggt aagctgcgcg gtctgctccg gcttccagtt
    37201 tttcagcgcc ggagcgtttt gattgagcac caccgccaca ttgaggcgtt tcaccgcaaa
    37261 gccgggatgc tgaatatgct ggacgctacg gtcgtaagag taatcgcgct tgctttcgct
    37321 gtgtttggat aacgcttcag gcgagcgatt ttcttccgta ccgttagtca tttgattcgc
    37381 cgcaaccggc ggacggttgc tgagagagcc gggcacaccc atcgccacct gattggtgtc
    37441 gctgtccagc acgctctctt cgcggtttat tttcggcgcg tcgccgtagt gttcctgagt
    37501 ttcgtcgatg gtgctgaggt cgagatccgg catcacgctg acacggtaat tcccgctgcc
    37561 gaccagcgaa tccagcacgt tcgccacgct ggcgtgggtt ttgtcctgaa tgtctttgag
    37621 gatctgatcg cgtttacggg tagcggcaga gaccgcctcg cccgcgccga tgccgtcggt
    37681 cagcagattt cccgactgat caaccacgct aactttgctg gcgtgcagcc ccggaatgct
    37741 gccggaaacc agatgcacaa tggcgttcac ctggtccata ttcagcttcg cgccgtagtg
    37801 cagacgcacc accaccgagg cgctgttttg cggctcatcg ctgaccacaa acgagctttc
    37861 ttcgttgagc gccagatgca cccgcgcgct ctccaccgcg tccagcgaca taatgctctg
    37921 cgccagctcc ccttcgaggc tgcgtttata gcgcacgttc tgcacgaact ggcggctgcc
    37981 aagcacttcg tctttgtcca tcagctcgta gccgctgggc aaaatcgcct gcacgccttt
    38041 ggcggcaagg gtcatgcgcg ttttcgacag cgcctcttcc ggcaccagta tctgcccgct
    38101 ttgcgggtca atgcggtagg tgagcttttc gccgtccagc acggtgacaa tctgcgacac
    38161 gggcagattt tcctggcgtc cgtagagcga gacgtagccg cgattgccgg tccacagcac
    38221 gctaacgata atcgccgtcg ccgccacggc aagccccaca cccgccatca gcgcccagcg
    38281 tttgttatct gcgagacgca gtcgaaacgc cggaaacgcc tgggttaact tctttatttg
    38341 tgcattcata gagttagatc gacatactca tcagatcgtt aaatccggtg gcgattttgt
    38401 tgcgcacctg caccagcgcg gagaaagaga gcgacgcctg ctggctggcg atcatcgcac
    38461 ctgccagatc atcgcttttg cccatgtcga tagccgtttg ctgctgctcc gccacctgct
    38521 ggaactggtc aacgtgaccg agcgcgccct gcataatgcg gtgaaacgaa acgtccgggt
    38581 cggtggaact gaaggtcatg gcgggcagca ccggcgcctg ggcgttggcc tgcatccgct
    38641 gcacgtcctg catgatctgc gcctgcatgg tgctgttgat tgtggtaatg ctcattttta
    38701 acttccgggt caggagaaaa cttcgatacc gtgcttgcgc atggaggcca gtcgatagcg
    38761 cagtgcgcgc ggggtaatac ctaacaggtc ggcgattttg ctgcggttgc cctgatattt
    38821 acgcatcagg tcagcgatat attgatactg ggcgcttcgt ccgtgctggc ctaaatcgct
    38881 ggtttcggca atgtgtacgg cgggctgagc aggccattgc agttcgctgt ggtcggcaat
    38941 gtcattttcc ggcaggccta aggcgtccgg gtaaatcacg ccgtcgcgat ttaaaatcat
    39001 tccccgctga atggcgttct ccagctggcg cacattgccg ggccagcggt aattcagcat
    39061 cgcacggcgg gtagattctg aaagggtgat atttttcacc agcaccgtgg agtatttttt
    39121 tataaatgac tctgccagcg gaataatatc gttcagacgt tcacgcagcg gcggcatggt
    39181 aataggaata accgccagac gataatagag atcttcacga aaacgcccgg cggcgacttc
    39241 ctgctcaagg tttttattgg tgcaggcaat caggcgaaaa ttgagtttaa tttgtcggtt
    39301 gctgcctaat cgctcaacct gttgttcctg caatacgcgt aatattttgg cctgtaacgc
    39361 cagcggcata tcgccaattt catcgagtaa taaggtgccg ttattcgcca gttccatttt
    39421 tcctggcaca ctggcaattg cgccggtaaa tgcgcctttg tcgtagccaa ataaggttgc
    39481 ttccagcatg ttttccggga tcgcagcgca gttcacgccg atatagggcg cgttttcatt
    39541 ttcaccaaag gcgactgtgt gaatatattt cgccacgcac tctttgcccg cgccggtttc
    39601 gccctgaatg agcaccggca cgttaaatgc ggcgacacgc tttgccagag taaaagcgtt
    39661 aatgctggaa gcggcagttg caataagttc agacataggg tgaacgatat aagtagcgac
    39721 aataatcagg atgttctgaa gcgtatcacc ggcgggatta acaagttcga attaaacgat
    39781 aataaaaata ttatttgttt ataaacaatg atttatccat gtaggccgga taaggcgttt
    39841 acgccgcatc cggcaattgt gcaccg
//
LOCUS       DAZGTR010000030        39324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGTR010000030 DAZGTR010000000
VERSION     DAZGTR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.030"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            240..659
                     /gene="umuD"
                     /locus_tag="ACHLCY_001752"
     CDS             240..659
                     /gene="umuD"
                     /locus_tag="ACHLCY_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0369030.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSSITISSEDTLDVFGVVIHVVKAMR"
     gene            659..1926
                     /gene="umuC"
                     /locus_tag="ACHLCY_001753"
                     /pseudo
     CDS             659..1926
                     /gene="umuC"
                     /locus_tag="ACHLCY_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(1972..2502)
                     /gene="dsbB"
                     /locus_tag="ACHLCY_001754"
     CDS             complement(1972..2502)
                     /gene="dsbB"
                     /locus_tag="ACHLCY_001754"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP0369031.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2648..4189)
                     /gene="nhaB"
                     /locus_tag="ACHLCY_001755"
     CDS             complement(2648..4189)
                     /gene="nhaB"
                     /locus_tag="ACHLCY_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP0369032.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4411..5130
                     /gene="fadR"
                     /locus_tag="ACHLCY_001756"
     CDS             4411..5130
                     /gene="fadR"
                     /locus_tag="ACHLCY_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP0369033.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5182..6714)
                     /gene="ycgB"
                     /locus_tag="ACHLCY_001757"
     CDS             complement(5182..6714)
                     /gene="ycgB"
                     /locus_tag="ACHLCY_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP0369034.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7044..8342
                     /gene="dadA"
                     /locus_tag="ACHLCY_001758"
     CDS             7044..8342
                     /gene="dadA"
                     /locus_tag="ACHLCY_001758"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP0369035.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8352..9422
                     /gene="dadX"
                     /locus_tag="ACHLCY_001759"
     CDS             8352..9422
                     /gene="dadX"
                     /locus_tag="ACHLCY_001759"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP0369036.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQVAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9808..11544)
                     /gene="cvrA"
                     /locus_tag="ACHLCY_001760"
     CDS             complement(9808..11544)
                     /gene="cvrA"
                     /locus_tag="ACHLCY_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP0369037.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11639..12553)
                     /gene="ldcA"
                     /locus_tag="ACHLCY_001761"
     CDS             complement(11639..12553)
                     /gene="ldcA"
                     /locus_tag="ACHLCY_001761"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP0369038.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12653..13264
                     /gene="emtA"
                     /locus_tag="ACHLCY_001762"
     CDS             12653..13264
                     /gene="emtA"
                     /locus_tag="ACHLCY_001762"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP0369039.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13266..14000)
                     /gene="ycgR"
                     /locus_tag="ACHLCY_001763"
     CDS             complement(13266..14000)
                     /gene="ycgR"
                     /locus_tag="ACHLCY_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP0369040.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14201..14455
                     /gene="ymgE"
                     /locus_tag="ACHLCY_001764"
     CDS             14201..14455
                     /gene="ymgE"
                     /locus_tag="ACHLCY_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0369041.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14633..15073
                     /gene="ycgY"
                     /locus_tag="ACHLCY_001765"
     CDS             14633..15073
                     /gene="ycgY"
                     /locus_tag="ACHLCY_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFP0369042.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15152..16849)
                     /gene="treA"
                     /locus_tag="ACHLCY_001766"
     CDS             complement(15152..16849)
                     /gene="treA"
                     /locus_tag="ACHLCY_001766"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0369043.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17169..18587)
                     /gene="dhaM"
                     /locus_tag="ACHLCY_001767"
     CDS             complement(17169..18587)
                     /gene="dhaM"
                     /locus_tag="ACHLCY_001767"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP0369044.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18598..19230)
                     /gene="dhaL"
                     /locus_tag="ACHLCY_001768"
     CDS             complement(18598..19230)
                     /gene="dhaL"
                     /locus_tag="ACHLCY_001768"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP0369045.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19241..20311)
                     /gene="dhaK"
                     /locus_tag="ACHLCY_001769"
     CDS             complement(19241..20311)
                     /gene="dhaK"
                     /locus_tag="ACHLCY_001769"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP0369046.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20539..22458
                     /gene="dhaR"
                     /locus_tag="ACHLCY_001770"
     CDS             20539..22458
                     /gene="dhaR"
                     /locus_tag="ACHLCY_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP0369047.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22558..25443)
                     /gene="ycgV"
                     /locus_tag="ACHLCY_001771"
     CDS             complement(22558..25443)
                     /gene="ycgV"
                     /locus_tag="ACHLCY_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0369048.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINEGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(26284..27375)
                     /gene="ychF"
                     /locus_tag="ACHLCY_001772"
     CDS             complement(26284..27375)
                     /gene="ychF"
                     /locus_tag="ACHLCY_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP0369049.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27492..28076)
                     /gene="pth"
                     /locus_tag="ACHLCY_001773"
     CDS             complement(27492..28076)
                     /gene="pth"
                     /locus_tag="ACHLCY_001773"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP0369050.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28354..28632
                     /gene="ychH"
                     /locus_tag="ACHLCY_001774"
     CDS             28354..28632
                     /gene="ychH"
                     /locus_tag="ACHLCY_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP0369051.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28687..30366)
                     /gene="dauA"
                     /locus_tag="ACHLCY_001775"
     CDS             complement(28687..30366)
                     /gene="dauA"
                     /locus_tag="ACHLCY_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP0369052.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30491..31438)
                     /gene="prs"
                     /locus_tag="ACHLCY_001776"
     CDS             complement(30491..31438)
                     /gene="prs"
                     /locus_tag="ACHLCY_001776"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP0369053.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31589..32440)
                     /gene="ispE"
                     /locus_tag="ACHLCY_001777"
     CDS             complement(31589..32440)
                     /gene="ispE"
                     /locus_tag="ACHLCY_001777"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP0369054.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPSVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32440..33063)
                     /gene="lolB"
                     /locus_tag="ACHLCY_001778"
     CDS             complement(32440..33063)
                     /gene="lolB"
                     /locus_tag="ACHLCY_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP0369055.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33277..34533
                     /gene="hemA"
                     /locus_tag="ACHLCY_001779"
     CDS             33277..34533
                     /gene="hemA"
                     /locus_tag="ACHLCY_001779"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP0369056.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALILWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34575..35657
                     /gene="prfA"
                     /locus_tag="ACHLCY_001780"
     CDS             34575..35657
                     /gene="prfA"
                     /locus_tag="ACHLCY_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP0369057.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35657..36490
                     /gene="prmC"
                     /locus_tag="ACHLCY_001781"
     CDS             35657..36490
                     /gene="prmC"
                     /locus_tag="ACHLCY_001781"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0369058.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36487..36879
                     /gene="sirB2"
                     /locus_tag="ACHLCY_001782"
     CDS             36487..36879
                     /gene="sirB2"
                     /locus_tag="ACHLCY_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP0369059.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36883..37692
                     /gene="sirB1"
                     /locus_tag="ACHLCY_001783"
     CDS             36883..37692
                     /gene="sirB1"
                     /locus_tag="ACHLCY_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP0369060.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYVQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37728..38582
                     /gene="kdsA"
                     /locus_tag="ACHLCY_001784"
     CDS             37728..38582
                     /gene="kdsA"
                     /locus_tag="ACHLCY_001784"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP0369061.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38731..38838)
                     /gene="ldrA"
                     /locus_tag="ACHLCY_001785"
     CDS             complement(38731..38838)
                     /gene="ldrA"
                     /locus_tag="ACHLCY_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0369062.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39266..>39324)
                     /locus_tag="ACHLCY_001786"
     CDS             complement(39266..>39324)
                     /locus_tag="ACHLCY_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0369063.1"
                     /translation="ILAGIITAAIVGWWRNRK"
BASE COUNT         9751 a         9811 c        10124 g         9638 t
ORIGIN      
        1 atatcaaaca atgtttaatg tcattatggc gaatgcttct attctatttt ttagccgggt
       61 aatatttttc atttctgctg gatgagcgta gtcgccagaa ggccgcgtga gcacaggata
      121 agagaacgaa aaatcagcag tctatgcagc gacaaatatt gatagcctga atcagtattg
      181 atctgctggc aagaacagac tactgtatat aaaaacagta tagcttcagg cagattatta
      241 tgttgtttat caagcctgcg gatctccgcg aaattgtgac ttttccgcta tttagcgatc
      301 ttgttcagtg tggctttcct tcaccggcag cagattacgt tgaacagcgc atcgatctga
      361 atcaactgtt gatccagcat cccagcgcga cttacttcgt caaagcaagt ggtgattcta
      421 tgattgatgg tggaattagt gacggtgatt tactgattgt cgatagcgct attaccgcca
      481 gccatggtga tattgtcatc gctgctgttg acggcgagtt tacggtgaaa aaattgcaac
      541 tacgcccgac ggtacagctt attcccatga acagtgcgta ctcgtccatt accatcagta
      601 gcgaagatac gctggatgtc tttggtgtgg tgatccacgt cgttaaggcg atgcgctgat
      661 gtttgctctc tgtgatgtaa acgcgtttta tgccagctgt gagacggtgt ttcgccctga
      721 tttatggggt aaaccggtgg ttgtgctatc gaataatgac ggttgcgtta tcgcccgaaa
      781 cgctgaggca aaggcgctgg gtgttaaaat gggcgatccc tggttcaaac aaaaagatct
      841 gtttcgtcgc tgtggtgtgg tttgctttag cagcaattat gagctttacg cagacatgag
      901 caatcgggtg atgtcgacgc tggaagaact atcgccccgc gtcgagattt acagtattga
      961 tgaggcattc tgcgatctga caggtgtgcg taattgtcgc gatctgactg attttggcag
     1021 agaaattcgc gcaacggtgc tacaacgtac ccatcttact gttggtgtgg ggatcgccca
     1081 gaccaaaacg ctggctaagc ttgccaatca tgcggcaaaa aaatggcagc ggcagacggg
     1141 ggggtggtgg atttatcaaa tctggaacgc cagcgtaaat taatgtctgc tctccccgtg
     1201 gatgaagtct gggggattgg acggcggatc agcaaaaaac tggacgcgat ggggatcaaa
     1261 accgttcttg atttggcgga tacagatatc cggtttatcc gtaaacattt taatgtcgtg
     1321 ctcgaaagaa cggtgcgtga actgcgcggc gaaccctgtt tgcaactgga agagtttgca
     1381 ccgacgaagc aggaaattat ctgttcccgc tcgtttggtg aacgcatcac ggattatcca
     1441 tcgatgcggc aggccatttg tagttacgct gcccgggcgg cggaaaaact tcgcagcgag
     1501 catcaatatt gtcggtttat ctccacattt attaagacgt caccatttga gctcaatgaa
     1561 ccttattacg gcaatagcgc gtcggtaaaa ctgctgacgc ccactcagga cagcagggat
     1621 atcattaacg ctgctacgcg atctctggat gccatctggc aagcgggcca tcgctaccaa
     1681 aaagcgggcg tgatgctggg ggatttcttc agtcagggag tcgcgcagct caatttattc
     1741 gatgacaacg caccgcgccc cgggagtgag caattgatga cggtaatgga tacactgaat
     1801 gctaaagagg gcagaggaac actctatttt gccgggcagg ggatccagca acaatggcag
     1861 atgaagcgtg ccatgctttc accacgttat acaacgcgaa gttctgattt actgcgggtc
     1921 aaataaatat agcggcagga aaaaagcgct cccgcaggag cgccgaatgg attagcgacc
     1981 gaacaggtca cgttttttcg ctttaaacgg ctgggaaatc accaccagca ccgcgacaat
     2041 caggtaagcg ataaaaatac cgagcagcca ctgcggcatt tccataccta aaaaatccca
     2101 ctgacgctcg gcgcaatcgc cagaggcgac aaacacttgc ggcacccatt tatccagcgg
     2161 cagccattcc gggaaacgaa ccataaaatc acaggtggca aacggcgaag gatagagctg
     2221 aagcatggtg tgctcgtaag ttaactgcac accgcggaac gcactataca accagataac
     2281 catcgctaca taacgcagcg gagttttcgg ggcgatcgcg ccaatcagcg cagcacccag
     2341 aacgccgaat aacgcgcagc gttcataaat acagagcacg caaggtttca gtaacatcac
     2401 atgctggaac cacagcgccg tcagttccag tgccagagca gtaaacgcca tcaacaacca
     2461 cgcgccccgg ccttgtgaac attggttcaa aaatcgcaac ataatcattt ccctgcaata
     2521 tgcatagagt gcgcagttta aaccaattca ttcgctgcgc caccaggagg cagcaaaaat
     2581 aggacgcaat tggacaatta tcgggcgaaa aggcaaaccg ggcatttata tgcccggtaa
     2641 gttgttatca aagcgttgct atccagccca tttgcataaa ccattcggtt acaggggcaa
     2701 gcgtaaactc gacgcagagc aagccgacga gtgtcagtac gagggtgtaa ggcagggcca
     2761 tccacaccat gcggccataa gagaggcgaa tcaatggcgc gagtgcagag gtcagcagga
     2821 acaggaacgc agcctgaccg ttcggcgtag cgacggaggg cagattggta ccggtattaa
     2881 tggcgaccgc cagcagctcg tattgcttca acgtgatagc gccactttcc attgccgctt
     2941 tcgcttcgtt gatataaatc gtccccacga agacgttatc cgaaatggat gacagcagac
     3001 cgttgaaaat atagaacagc gacagctgag catgttccga tgcctgcaac acaaactgaa
     3061 taattggcga aaacagttgt tggtcgataa tcaccgcgac taccgagaaa aacaccgtca
     3121 acagtgcggt gaatggcaga gattcggtga aggctttacc gatagcatgt tcatcggtga
     3181 caccggtcaa tgatgtcgcc agaataatga ctgacaaacc aatcaagcca acttccgcca
     3241 gatgcaacgc cagcgcagtc accagccaga cgccaataat cgcctggaca atcagacgga
     3301 ttttatcctg acgggtacgc tggtggcggc tttgatcgtc aaactgttgc aacacttcgc
     3361 ggactttctc cggcagcgtt tcaccgtagc caaaccaacg cagcttctct accagcaggc
     3421 aggttaacag gccacaaatc agaaccggaa cggtcaccgg cgacatgcgc aggaagaaat
     3481 cgccaaaatg ccagccagcc gctttagcga tgatcaggtt ctgtggttcg cccaccatgg
     3541 tcatcacgcc gcctaatgca gtgccgacac cggcatgcat catcaggcta cgcagaaagc
     3601 cacggaactg ttccagaacc actttgtaat gcttgtcgat atgactatcg tcttgcaggt
     3661 cggtatcttc agtacgggaa gaggctacgc ggtgataaat accataaaaa ccgactgcaa
     3721 cgctgatcac caccgccacg acggttaagg catcgaggaa cgcggagagg aacgccgccg
     3781 ccacgcaaaa agagagcgac agcagcattt tggagcgaat gcttaacagc aaacgggtaa
     3841 atatgaacag caacagctgt ttcataaaat agatacccgc caccataaac atcaataaca
     3901 gcaagacttc aagatttgcc gccacctctt cacggacgtg ttccgcgctg gtcatgccga
     3961 tgaataccgc ttcgatagcc aacagaccac cggggagcag cgggtagcat ttcagggcca
     4021 tcgccagagt gaaaataaat tccgctacca gcaaccagcc cgcgacgaaa gggctgatga
     4081 ggaaaattaa cgggtttacg attaagaaaa tgatgagggc gagtttgtac cagtcggggg
     4141 actggcccaa aaaattgcgc catagcgcgc ggccccagga gatctccatg atggtttccc
     4201 ttaccttaca aataatcaat gatgttttta tgtttaaacg caaagcttaa cggtcaggca
     4261 ggagtgaggc aagtcttgat agtcaagggg aaagagatgc ggaaaatgaa gccttgatcc
     4321 cttttttctt ctttttgtct gctatcagcg tagttagccc tctggtatga tgagtccaac
     4381 tttgttttgc tgtgttatgg aaatctcact atggtcatta aggcgcaaag cccggcgggt
     4441 ttcgcggaag agtacattat tgaaagtatc tggaataacc gcttccctcc cgggactatt
     4501 ttgcccgcag aacgtgaact ttcagaatta attggcgtaa cgcgtactac gttacgtgaa
     4561 gtgttacagc gtctggcacg agatggctgg ttgaccattc aacatggcaa gccgacgaag
     4621 gtgaataatt tctgggaaac ctctggttta aatatccttg aaacactggc gcgactggat
     4681 cacgaaagtg tgccgcagct tattgataat ttgctgtcgg tgcgtaccaa tatttccact
     4741 atttttattc gcaccgcgtt tcgtcagcat cccgataaag cgcaggaagt gctggctacc
     4801 gctaatgaag tggccgatca cgccgatgcc tttgccgagc tggattacaa catattccgc
     4861 ggcctggcgt ttgcttccgg caacccgatt tacggtctga tccttaacgg gatgaaaggg
     4921 ctgtatacgc gcattggtcg tcactatttc gccaatccgg aagcgcgcag tctggcgctg
     4981 ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg cgcacgatca ggtgtacgaa
     5041 acagtgcgtc gctatgggca tgagagtggc gagatttggc accggatgca gaaaaatctg
     5101 ccgggtgatt tagccattca ggggcgataa tcccttccgt ttaaagagca aacccctcaa
     5161 acgaggggtt ttttgttgtt tttacagatt tcccattctt ggcgggcaac gttccagcaa
     5221 ctcgatgctg ccgtcttcgt tttgctgttc gagcatcaca tcaaatcccc acaggcgatg
     5281 cacatgcttc agcacttctt tgcgcccccg atccagcggt gcgcgattat gtggaatgta
     5341 acgcagcgtc agcgaacggt cgccgcgcaa atccacgttc cagatctgaa tattcggctc
     5401 cagattactt aagttatatt gcgacgataa ccggttacgg atctcccgat aaccttcttc
     5461 attatgaata gcggaaatct ccagataatt atgccgatca tcatccagca cggtgaagaa
     5521 gcggaaatca cgcatcactt tcggtgacag aaactggctg ataaaactct catctttgaa
     5581 atcacgcatc gcgaaatgca gcgtttccag ccagtcggaa ccggcgatat ccgggaacca
     5641 gtatttgtct tcttccgttg gcgactgaca aatccgttta atatcctgga acatggcgaa
     5701 cccgagggca tacgggttga tgccgctgta ccacgggctg ttataggggg gctggaagac
     5761 cacattggtg tggctgtgca aaaactccag cataaaacgt tccgttactt tcccttcatc
     5821 atacagatgg ttaaggatgg tatagtgcca gaaggtcgcc cagccttcgt tcatcacctg
     5881 agtctgtttt tgcggataaa aatactggct caccttacgc acaatacgca ggatttcacg
     5941 ctgccatgat tccagcagtg gcgcattttt ctccataaaa tagagcaggt tttcttgtgg
     6001 ttcggacgga tagcggcgcg cttcagcaac cgttttctct tcctcgcgct tcggcagggt
     6061 acgccagagc atattgacct gactttgtag atactcttcg cgacttttct gccgggcttt
     6121 ctcttcttgc agtgagattt tttgcgggcg tttgtaccgg tccacgccgt agttcatcag
     6181 cgcgtggcac gagtccagaa gccgttctac ttcatcaacg ccataacgtt cttcgcactc
     6241 ggtaatatat ttacgggcaa aaatcagata atcgacaatc gaactggcgt cggtccaact
     6301 gcggaataag taattgtttt taaagaaaga gttatgccca tagcaggcgt gtgccatcac
     6361 cagcgcctgc atggtaatgg tgttctcttc catcaggtaa gcgatacacg ggttagagtt
     6421 aatgacgatt tcataggcca gtccttgctg accgtgctta tacagccgtt cagtttcgat
     6481 aaacttttta ccgaatgacc agtgcggata gttaattggc atgccgacgc tggagtaggc
     6541 atccatcatc tgttctgagg ttatcacttc aatctggtgc gggtaggtat ccagccggta
     6601 gagtttcgcc acccggtcta tctctgccag ataaacatcc agcaggtcga acgtccagtc
     6661 gggtccatcg ctcaaacgtg tggtgtcctt attcatagaa tcgatcgtcg ccatacgcgc
     6721 acctcattgt tgtcggcgct ctctgtgtgg agcacctcat ttcaagcata gaacacctgt
     6781 taaaaaccgc gtcgccggag aatttttttc tttgcgattt cttattatca gagtgccact
     6841 aatccgcttc tgaacggaat tttatgctgg ataaaaaggg cgttcagcag gagatactaa
     6901 agacgccata ttgccgcaga gtcagggaga tgtgagccag ctcaccataa aaaagccgta
     6961 tattgaataa tattttcaac tgagttatca agatgtaatt agattattat tcttttactg
     7021 tatctaccgt tatcggagtg gctatgcgag ttgtcatact gggaagtggt gtggtaggcg
     7081 ttgccagcgc ctggtactta aatcaggcag gacatgaggt caccgtcatt gatcgggagc
     7141 cgggggccgc cctggaaacc agcgccgcga atgccgggca aatctccccc ggatatgctg
     7201 caccgtgggc ggcaccaggt gtgcctttaa aagcgatcaa atggatgttc cagcgccatg
     7261 cgccgctggc ggtccgtctc gatggtacgc agtttcagtt gaaatggatg tggcaaatgt
     7321 tacgtaactg cgacaccagc cactacatgg aaaacaaagg gcggatggtg cgtctggcgg
     7381 aatacagccg tgattgcctg aaagcattgc gcgccgaaac caatattcag tatgaagggc
     7441 gtcagggtgg gacgctgcaa ctgttccgta ccgaacaaca gtatgaaaat gcgacccgcg
     7501 atatcgccgt gctggaagat gccggcgtac cgtatcagct gctggaatcc agccgcctgg
     7561 cggaagtgga gcccgcgctg gcagaagtag cgcacaaact gacgggtggt ctgcaactgc
     7621 ccaatgatga aaccggagac tgccagctgt ttacccagaa tctggcgcgg atggcagagc
     7681 aggcgggggt taaattccgc tttaatacgc ccgttgacca actgctttgc gacggcgagc
     7741 aaatctacgg cgtgaagtgt ggcgatgaag tgattaaggc cgatgcgtat gtgatggcgt
     7801 ttggttctta ctcgacggcg atgctcaaag gcattgttga tattccggtg tatccgctga
     7861 aaggctactc gctgactatt ccaattgcgc aggaagatgg tgcgccagta tccactattc
     7921 ttgatgaaac ctacaaaatc gccattaccc gtttcgataa ccgcattcgt gttggcggaa
     7981 tggcggaaat tgttggtttt aacaccgagc tgttgcaacc gcgtcgtgaa acgctggaaa
     8041 tggtggttcg cgatctctat ccacgcggcg gtcatgtcga gcaggcgact ttctggacgg
     8101 gtttgcgccc gatgacgcca gacggtacgc cggttgtcgg gcgtacacgc tttaaaaatc
     8161 tgtggctgaa taccggtcac ggcacgctcg gctggacgat ggcttgcggt tccggtcagt
     8221 tgttaagcga tctgctctct ggtcgcacgc cagcgatccc atatgaggat ctaagcgtag
     8281 cgcgctacag ccgtggattt acgccatcac gtccgggcca tttacatggc gcacacagct
     8341 aaggaaacga gatgacccgt ccgatacagg ccagcctcga tctgcaggca ttaaaacaga
     8401 atctgtccat tgtccgccag gccgcgccgc acgcgcgcgt ctggtcggtg gtaaaagcga
     8461 acgcttacgg gcatggtatt gagcgtatct ggagcgcgct cggggccacc gatggctttg
     8521 cattacttaa cctggaagag gcaataacgt tacgtgagcg cggctggaag gggccgatcc
     8581 tgatgctgga aggatttttc catgctcagg atctggagat ttatgaccag caccgcctga
     8641 ccacctgcgt acacagcaac tggcagctca aagcactgca aaatgcgcgg ctaaaagcac
     8701 cgttggatat ttatcttaaa gtgaacagtg ggatgaatcg gttgggcttc cagcccgatc
     8761 gcgtgcttac cgtctggcag cagttgcggg caatggcgaa tgttggcgag atgaccctga
     8821 tgtcgcattt tgccgaagcg gaacatcctg atggaatttc cagcgcgatg gcgcgtattg
     8881 agcaggtggc ggaagggctg gagtgtcggc gttcgttgtc caattcggcg gcgactctgt
     8941 ggcacccgga agcgcatttt gactgggttc ggcctggcat tattttgtat ggcgcttcgc
     9001 cgtccggtca gtggcgtgat atcgccaata ccggattacg tccggtgatg acgctaagca
     9061 gtgagattat tggtgtccag acgctaaaag cgggtgagcg tgtgggctac ggcggtcgct
     9121 atactgcgcg cgatgaacag cgaatcggca ttgtcgccgc agggtacgcc gacggttatc
     9181 cgcgccacgc gcctaccggt acccctgttt tagtggacgg cgtgcgcacc atgacggtgg
     9241 ggaccgtctc gatggatatg ctagcggtcg atttaacgcc ttgcccgcag gcggggattg
     9301 gtacgccggt tgagctgtgg ggcaaggaga tcaaaattga tgatgtcgcc gccgctgccg
     9361 gaacggtggg ctatgagttg atgtgcgcgc tggcgttacg cgtcccggtt gtgacagtgt
     9421 aacttgttgt aagccggatc ggaggcagcg tcttctggtt gcgaaaaaat catccatccg
     9481 gctggtcagc aactgtagtt gttaatgtga cagagccatt gcccatgata gtgtccatta
     9541 aaagaatgga cactatttcc ccggaacctg aactcaccgc acaggcgttc tacataaaac
     9601 gcttacgctt cattgttgac tcgaactcga cttcagataa atcacgcttt acccttgatg
     9661 gagcctgtac atagatttgt gtaattgcct gattttgata tgttcaattc aacatcaaat
     9721 gaaggttaaa ttatggacga caaacaattg caggctcagg ctgcgttcag caaagcatcg
     9781 caaccggcga tagatgcttc attaaattta agattcagct tcctcttcag ccacccgtac
     9841 gccaatcttc aacacttcat tatcttcttt ctcggccacc gtccagatca tcccggcaaa
     9901 ctctacctgg tcaccgacaa ccggtgccgc gcctaacaac tgctggacaa tttcacccag
     9961 cgtttgctgc ttatcacgat attctcgccc gtcttccagg ccatatatca gcgcaacatc
    10021 agcatatttg gcgctggctt cgagaatgaa gtcaccaaag aagcgttgat ccagcgcgac
    10081 cggcggcgac tggctgaaca gtttaccgag cgccggaaga tcgcgttccc gaccaattac
    10141 acacaacaca tcgccttcgc gcagtcgggt gctgccggta ggatgaagca actggttatc
    10201 acgaaacagt gccgcaatac gcgtctcttt tggcatatgc aaatcgcgca gtgccgcgcc
    10261 cacgcaccat ttatcggcac tcaattgata aacaaactgc tcccacggat tttccggatg
    10321 aatatccagg ccaacgcgtg acaccggacg tcccactggc ggaaccacca ctttggcttt
    10381 tttagccgcc cacgagagtg atgttccctg caagagcagt gaaaccagaa ccacaaagaa
    10441 ggcgacatta aagaacagac gtgcattctc cagccccgcc atcatcggga acacagccag
    10501 gatgattggc accgcgccgc gtaatcctac ccagctgata aacacgcgct cacgcagatt
    10561 gaaaccgcgg aaggggagca atccagcaaa taccgaaagg ggacgggcga agaatatcat
    10621 ccatgccgac aaaatgagcg ccggaatggc aataggcagc agatcgcttg ggttaaccaa
    10681 cagccccagc accaggaaca tggcgatttg cgccagccag gcgaggccgt cgaaattttg
    10741 caggatgccg tagcggttgc gaatcggccg attacccagc agaaaaccgc acagataaac
    10801 agccagaata ccgctgcctt ccagcgccgt ggttaaagca aaaattaaaa taccgccgct
    10861 taatgccagc aatggatata atccggcggg cagggcgatg cgattaatca tttgcagcag
    10921 taaataacca ccaccaagtc cgatgacaat cccgaggcca aattgctgca gaatatcgac
    10981 cacgaacatc caactgacac tgctttcatg ttgctggatc atcgcaatca gggtaatcgt
    11041 cagaaagacc gccattggat cattactgcc ggattcaatt tccagcgtcg agccaacacg
    11101 ttcgttaagc cccttaccac ccagcagaga aaagaccgct gcagcatcgg tagagccgac
    11161 gatagcgccg attaataagc cttcaatcaa atcaagatta aacagccacg ccgccatcat
    11221 gccggttaaa ccagaggtga taagcacgcc cagcgtcgcc agcgacagtg ccggtcctaa
    11281 cgccacacga aaggaactgg cctgagtgcg catcccgccg tcgagcaaaa taatcgccag
    11341 tgccaggtta cttaccatgt aggcgaaggg gtaattatca aacgggatac cgccgacgcc
    11401 atcgactcct gccagcatgc cgatcgccag aaaaataacc agaataggaa tgccaagacg
    11461 ggaagaaaat gaactaagta atatactgct ggtgacaagg atggatccta agatgaaaag
    11521 gctaattatt gttgtggcat ccaacggtcg gttactcctg attacgctgt ctcttatata
    11581 aaccctacca tattagcggc agagacagcg ttttacttag ccctgaagcg tgattttttt
    11641 acattttaag aacaggatga ccgctaatcg tcaactgagt gccttcccgg gtgttattca
    11701 gaatggcatg tgcgcccaac ggcagcgtaa ccgtgcgttg ttcatgacca aaatcgaggc
    11761 cggtaatgag tggaatcgac aggcgggaac gcaaaaacgc gtacactgac tccaggttgt
    11821 aacctgcgtc ataatcattg ggcgtgctgc cgctaaagct accgagaata atcgccttct
    11881 gacgtggcaa aattcccgca tgatagagct gcaacagcat acgttcgacg cggaaagggt
    11941 gctcgttaat atcttccagc accagaatac cgttctcaat ttttggcatc cacggtgtac
    12001 caatcagtga aatcagcatc gcaagattgc ctccccacaa cgtgccttcg gtctgacatg
    12061 tcgggccttc cccttgccat tcaatggtga aggtttcatt gcgtaacgcc agccagaaat
    12121 ggtgctcggt aaaggcgttc agttcatccg cgccaaagtt tgctaccagc atggggccgc
    12181 taaaggtgat gacattgcct tgcgccagaa gaccgcactg aatggcggta aaatcgctat
    12241 gcccgcatat gagcaacggg tcatattgtt ggcgagcaac cagtgcctgc cagtcaatat
    12301 ctgccagtaa acgactggca ccgtaaccgc cgcgtacagc cagcacgatg gtgttggggg
    12361 tggtcagtct ggcaagggaa ttgagatctt ccaggcgctc cgtttccgta cccgcaaaac
    12421 gctcacaacg acgggcaata acctcgacgt tattgacctg atgcccctcg tcggtaaggc
    12481 gctggatacc acgcagcgcg gcgtgctgtt taatgcagta acccgatggg gcaattaagt
    12541 gaaacagaga catggcaatt ccttgctgac aacagaaacg aaatgtatat catgccgctt
    12601 aggtgtgccg ttgtcacctc aacggcgatt ccaggctata aggatagaag aagtgaaatt
    12661 gagatggttt gcctttttga ttgtgttatt agcgggttgt tcatcaaagc atgactatac
    12721 gaacccgccg tggaacgcga aagttccggt gcaacgtgcg atgcagtgga tgccaataag
    12781 ccagaaagcc ggtgcagcct ggggcgtcga tccacaattg atcacggcga ttatcgctat
    12841 cgaatcgggt ggtaatccga atgcggtgag taaatcgaat gccattggtt tgatgcagtt
    12901 aaaagcttca acctccggac gtgatgttta tcgccgtatg ggctggagtg gtgagccgac
    12961 gaccagcgag ctgaaaaatc cagagcgtaa tatttcaatg ggggcggctt acctgaatat
    13021 tctggaaacc ggcccgctgg caggcattga agatccgaag gtactgcaat atgcgctggt
    13081 ggtgtcatac gctaacgggg caggtgcgct gctacggact ttctcgtcag atcggaaaaa
    13141 ggcgatcagc aaaatcaacg atttggatgc tgacgagttc ctcgatcacg tagcgcgaaa
    13201 tcatcctgcg ccgcaggctc cgcgctatat ctacaaactt gagcaggcac tggacgcgat
    13261 gtaaatcagt cgcgcacttt gtccgctttt tcccgggctt ctcgctcgag agagaaaata
    13321 atccgctgta attgccgctc caccgtcggg ctgacgttaa gaaaacggaa gctcagacgg
    13381 ggagtggtga tggtttcatt cttgccatca atcactttgc gctcgctgat ggagattaac
    13441 tgggcgtcaa agtgaaaaac accccattgc cccatgttga cttcaatctg agcgaagcgc
    13501 atgccttctt gtaattcggc aggctttgct gtttccagta atgcgcccat gccgcctaac
    13561 gacaaatcat acaggcggaa acgtaacgta ctgttatccg ccagtttggt ctggcaaaaa
    13621 taaggcggat ggagtggggc ggagatgcgg aaatatcggc gtcgttgtac aaaccataag
    13681 gtgggaggcg gtacggtgat aaatgccgga agctgcaagt attcactctg ctgtagttgt
    13741 tcaacagtaa actcgacttt cgcaccctga gtttcggcgg taatggtaat gtgctgtgcc
    13801 tttagcacgg cgatgttgtc ttcggcttga ctgccgaaat ccagcaccag tttatccggg
    13861 gttattgcca gtattttgct gatcagctgc ccgccattcc aactgagacg caaaggaatt
    13921 gcggctttgt gcaaatcgcg taacacgccc aggacggcta acggattttg tttcaggaac
    13981 tgctcatggt aatgactcac gcggaaaaac tcctgactga caaactgtct ttggtttatc
    14041 ggtcacagtt aacaaaactt aatacaaatg cgtgaatatt ttttacatgt tgttcttaaa
    14101 tcagccgaca tacgcccagc atcgatcccg cccttgccta tacttagagc gttgacgtaa
    14161 gcatttcttg cgtccgattc atcgaaacga gggcctgaac atgggaatta ttgcctggat
    14221 tatttttggc ctgatagccg gcattatcgc caagctaatc atgccggggc gtgatggtgg
    14281 tggatttttc ctgacctgta ttctcgggat agtcggtgcg gtggtcggcg gctggctggc
    14341 gaccatgttt ggcattggcg gctccatcag tggttttaat ctgcacagct tcctggtggc
    14401 ggtggtggga gctattctcg ttctgggcgt attccgcctc ctgcgaagag aataagattt
    14461 tcataaggcg gatagcgata cagatgccgc tatccgcttt cacatcagaa cgtatactcg
    14521 acacctgctt tacgggtgga acaaatcaat atagcactta gatgcccgat aaagtcggtt
    14581 cccggtaaca acaacagaat ccttttttaa ttattgtttc gttgtttttg gtgtgattca
    14641 gaaaataaat attttagaca gtagccagtc aggcgttaaa aatggggcgg atagtgaggc
    14701 ggttttggct gcgcttattg aacatttaga attgatcaat ccttctggca gattaagcca
    14761 aaatacccgt tcggcaatgc ttcagttacg cgaagaggaa tggtctgaat tctttttttg
    14821 gttgctaaat tctctggagt gtctggatta tgtgattatt aatttaactc cagaaagcaa
    14881 aaaaacgttg atgagtgaac accgtaacaa tattcaggta gcaatcgatg ctttatatag
    14941 gcaacgtcgg tgtaaatcac cgggtgatga aagtgaaaca cttacccgta gaaatgacgc
    15001 tatcttcggc aaccacgtat ggcaaacatt tgcgcaatac ttcccgccag gacttgaaaa
    15061 accgtctgtg tagaaaacac catagctatg gtgttatctg ccgacaacgc catccggaga
    15121 agccgaatga cgttgtcgga gtaagcgctg gttaaggtgt gggttgtgcc tctttggttg
    15181 agggttgcgt cgttgctgac ttaacggtcg gacgcgtcgc cggaacattg tcacacggtt
    15241 gctctttcgg gcagatcaaa tccagcattt tcagcgtcac gccattggtc cagccaaagc
    15301 catcctgtaa tggatattcg ccaccgccgc cccccgttcc ggtggtgctg acatcatatt
    15361 tttccaccag ctttttctcc cggtcatagg tgtgctgaac attggtcagg aagtgccagc
    15421 taatgtccat cgccacctct ttttgcccgt agttttgtaa tccttctgtc gcgacccact
    15481 gtaacggtgc ccagccattt ggcgcatccc attgttgccc acttttcacc gacgtggtgt
    15541 tcaggccgcc gggttgcagc agatgtgttt tcgtcgccgt cgccattttg ttggcgcgat
    15601 ctttcgctgc cgcattgacg tacagcggga acagggcggc cgcggttaac tgattgcgca
    15661 ctttatgact tttcaggtcg taatcggcat accagccttg ttgatcgttc cacatgtatt
    15721 tttcgatccc tttttgacgg gcatttgcca gcgtttcgta ctggtttgcc atcgcgttat
    15781 ctccggcagc tttgctggcg cgggcgagga ttttttccat tttaaacatc aggctgttca
    15841 gatcgaccgg tacgatgctg gtggtgcgta aggtatttag ttgatgcggg ttgtccatcc
    15901 agcgcgagct gaaatcccag ccagacgcag cggcagagcg caggtcgcgg taaatttcag
    15961 tggcaggtcg attcggattg cttttggcgg tggcaatatc ttccacccat gactctggtc
    16021 gtggcgtatc gcgatcgtcc cagtagcggt tgagaagggt accatcctga agttttacaa
    16081 cgcgtttttc ctgttgtccg gcttgcaggt tttcaacacc gtccatccag taagcatatt
    16141 ctttttgcat ttgcggcagg tattgcttca acgcggcatc gccttcatgc tgcgccagta
    16201 actctaccat cagggcaaag aagggcggtt gcgagcggct taaatagtaa ctgcggttgc
    16261 cgttgggaat atgaccgtaa gtgtctattt catgagcaaa attggccacc atatccgcga
    16321 ctttatccca gtgaccgctt tcggcaagtc ctaacatggt gaagtaactg tcccagtaat
    16381 atacctcgcg aaaacgtccg cccggcacga cataaggttc cggcagcggt aacagagaat
    16441 cccatttttc ggtgttttcg gtagaacgcg ttaataccgg ccaaagtccg tcaatatgtt
    16501 cgcgcagtga ctgcccctct ggcggaacat atttctcgcc ctctttcggc agggtgaaat
    16561 tgacgttaac gaaatggcgc agatcaaatc cgctctggtt ttgctgcatc cgataatcag
    16621 caaggatcat cagcggatcg ctgttcggca cggcatcggc aaaggttttt tggtccggaa
    16681 aaagtttggc gttttgcaca tcattaaaca gcggccctaa taaaatatca ggcggctgtg
    16741 gtgttaccgg tgtttcttct gcctgcaccg atagcgcagc gaaacacaaa aagatacagg
    16801 ctggaattaa cgccattttt tgcgggcgag aaggtgcggg ggatttcatc aatcattctc
    16861 ctttggcgaa accgaataaa acggtattca agtatcagaa aaccttagtt caggatcgca
    16921 ctagctgcat gatcgaaatc tcattctgcg aactgtcagg cattttagaa aagggaatta
    16981 aagggataaa taacggtgaa atagagtgat caaataacgt ctgtttatta gccagtcagc
    17041 gacgttgggt tcgcgattag cgaagaaaaa tcatcataaa ttggtaaaaa atataacagg
    17101 tagttaaaaa acatcagtgc tgggtaaatt gccggatgac atcagaacga tgccatccga
    17161 acggtggctt aaccctgacg gttgaaacgt tgcgttttaa cgtccagcgt tagcgtttct
    17221 tctggttgta tcgcatacag tttctcaccc tgctggcaaa tccagccaat ccccagttca
    17281 cgggcgatta gggcgctgtg ggataccgga cttccggcgc taaggcagat accttttaca
    17341 accgccggat ccagttgcag tactgtggaa ggataaatgt tctccgccag tagaatagtt
    17401 ggcgagttaa actgcgggag ttcttctttc gtttgggtca ggtggaccag ggtgcgatgc
    17461 agaagatcgt ccacatcaat atagcgagct tgtagatatt catcatccag ttgctggtat
    17521 tgctggctaa gttctttaag aacttgctgc caggcatatt ctgccgtgca atgttcatgc
    17581 tgaaggagtt cgcttgccgc cgccagcagt tccggatcat ctaacagtgt atggtgacca
    17641 gaaaagattg cggcaatatc gtcaagcccg ctggcttctg ctttcgctgt taacgtcatc
    17701 agatctaata acgtgaagtc aatagcctgg cgtaatcgat cttgttcttc ttccacggtc
    17761 agggttgatt ttgcctgtac cgtacataaa actggttgat aataaaaggc tttacccgaa
    17821 acaggcggaa cgggacgcag agtaggtgga gcgacttcct ccgtttcacc aaagttatct
    17881 tcagccagct gacggaaagc gatcagtgcc tcttcagctt ctggcccttt cgcaatcagg
    17941 cgcagcgtat cgttatagcg aacttgtagt aacgcaatct ggttaatact ctctggtgtg
    18001 acgcatttgc cgtttttttc cagcaacata tcggcattaa atgtcgataa ggtataaacc
    18061 agccgggagg ccggacgtac atgcaggccg ttacggtttt ttatgaccac cgccagagaa
    18121 cgggcttctt catcgtacgc aggacatgtg tcagagattt cagtgtcgga ggacggtaaa
    18181 cccagttgtt cacgtttggc ttccagcgca tgcatagcgt caaagataac tttgtcgata
    18241 tccgctcccg aggccgcgct gaccgttgct gccagtgtac cttcgaccaa cggcgcagca
    18301 cacaaacgta cttttgcggc gatcttggga gccagcaatt ccagcgcagt ttcagcactc
    18361 aataatgcgc tacccatatc catcatgacc agcacatggt cggcatcagc aacagattcg
    18421 atggcctcca tcactttgac ggcatcggta ccaataggat tttgtggatc gtcaattccc
    18481 gcggcaatgg cgattttaca actatcactc attaacatct gacgggctaa ttcaccgaca
    18541 ccttctccca gtcggctgct atgtgaaact atgaccaggt ttaccatcac caattcctta
    18601 ctcttttgcg gctaacgcca acatttgcat cataaacatc accgaggtcg cgccgggatc
    18661 ctggtgacca atactgcgtt caccgagata actggcgcgg cctttgcggg cttgcatcgt
    18721 aatcgtactt tgtgcagcgg attcggcgat tctactggca gcttcgagcg ccaccggaac
    18781 agagagattt tgctcgctgg actgacgtaa cgattccacc accggcaccc acacatcaca
    18841 catggtttta tcgccaggtt cggctttccc gcgactgatt acgccgtccg cgccatcgcg
    18901 gaacatctga taaagctctt ccagtgtcag gctttgccgt gcctgggtcg cctgtgcggc
    18961 gcggataaag aaggtaccga acagcggacc actggcacca ccgacgctgg aaagcagcgt
    19021 cataccggta ttcttgagaa tgaaaccgat atctttatct gcgatagcag ggagtttttc
    19081 caccactttg ctaaagcctc ggttcatatt tagcccgtgg tcagcgtcgc caatttcgcg
    19141 atccagtccg gtaagatact cgctctcggt gctgaaaata tcgccacaac gagtgagcca
    19201 gttaacaatt tgagttctgc tcagtgacat tgctttctcc ttatttaccc cagttaaggg
    19261 ccggggtgtg gaccggggcg tcccagagtg ccagcgtttc gtcatcaact ttcagtaagg
    19321 tgattgagaa accggtcata tccagtgagg tgcagtacgc gccaattaaa ttacgttcga
    19381 tagtcaatcc cgcttgctgg caacgtgtgg tcaggcggtt atagacgccg tacagctcag
    19441 aaagcggagt tgcgccaaga ttgttaacca gcgcaatcac ccgatcgcca gactggagcg
    19501 gttgtttggt ttgttgttct tcctgccaac tgccttgttg ataatcccag aaacgcaaag
    19561 tgcgatggta tgagccattt tccagcaggg tgtcgaacat ttcatcgacg gtttgatcaa
    19621 gggaagagaa ggagcggcgg tcaatacccg gctcaccatg aatgccgacg ccaaactcca
    19681 tctcattatc cgccagggta aaagaaggtt tgcccgcggc aggaacggta caggcaccga
    19741 gagcgatacc tattgagtgg ccttgattat tcaacttacg ccccagttcc gcacaggcgt
    19801 ccagtgagtc gccacgctcc gccgctgcgc ctacgagttt ttcaattaat acggtgttgg
    19861 caacgccgcg tcgcccggcg gtataaagac tgtcttttac cgcaacgtcg tcatcaatga
    19921 ccacagtggt cacttttacg ccgctatcgt gcagtaactc ggtcgctgtt tcaaagttaa
    19981 gaatatcgcc ggtgtaattt ttgataatca acagtacacc ttcgccgcca tcaatttgca
    20041 tggagcattc aaagatttta tcgggcgtcg gtgaggtgaa aatttcgccc ggacaggccc
    20101 ccgaaagcat cccctgaccg atataaccac agtgcatcgg ctcgtgtccg ctgccgccac
    20161 ccgacagcag ggcgactttt cctgcaacag gggcatcagc tcgggtgaca tacaccggat
    20221 cctgatgcag tgtcagcgat ggatgcgctt tcgccagtcc tgccagttgt tcgtccagta
    20281 cgtcttgcac atcattgatc aattttttca ttattttgct ccagcaatta cggtagggca
    20341 tggatgatgt tcaacgacac ggcgaccact gactgccgat gaatccattg tgcatcaggc
    20401 aagggaaaag aaaattagcc caaattatgt ttcatagtga aacatatgct ttaatgaatg
    20461 ttccatattg aaacttttac gtgtattaat acttaaaatt gcgagccgga acaccttttg
    20521 tcataaggga tgcgggatat gagtggcgct tttaacaacg atggtcgggg catatctccc
    20581 ttaattgcaa cctcctggga gcgatgcaat aagctgatga aacgggagac atggaacgta
    20641 ccacatcagg cccagggcgt gacatttgct tctatttatc ggcgtaagaa agcgatgctg
    20701 acgctcgggc aggctgcgct ggaagatgcc tgggaatata tggcaccgcg agagtgtgcg
    20761 ctgtttatcc tcgatgaaac cgcctgcatt ctcagccgta atggcgatcc gcaaaccttg
    20821 cagcagctaa gtgcactggg attcaatgac ggcacgtatt gcgccgaggg aattattggt
    20881 acttgtgcgc tatcgttagc ggctatctct ggtcaggccg tgaaaacgat ggccgatcaa
    20941 catttcaaac aggcactctg gaactgggcc ttttgtgcaa cgccgttgtt tgacagcaag
    21001 ggccgattga cgggaacaat agcgctggcg tgtccggttg agcaaactac cgcagctgat
    21061 ttgccgttga cgttggcaat cgcccgcgag gtcggaaatt tactgctgac ggacagtttg
    21121 ctcgctgaaa ctaaccgtca tttaaatcaa cttaatgccc tgttagaaag tatggatgat
    21181 ggcgtgatta gctgggacga gcagggtaat ttgcaattta ttaatgccca ggcggcgcgg
    21241 gtcttgcgcc ttgacgcgac ggcaagtcag ggaagggcaa tcactgaact cttaacgtta
    21301 cccgccgtat tgcaacaagc aataaaacag gcacatccgc tcaaacacgt agaagcaacc
    21361 tttgaaagtc agcatcagtt tattgatgcg gtgataaccc ttaaaccgat aatagaaacg
    21421 cagggaacca gctttatttt gttgctccat cctgtggaac agatgcggca gttgatgacc
    21481 agtcaattag gaaaagtcag ccataccttc gctcatatgc cacaggacga tccgcaaacc
    21541 cgccgcttga ttcattttgg tcgccaggcg gcgcgcagta gctttcctgt cctgctttgt
    21601 ggagaagagg gcgtgggaaa ggcactgcta agtcaggcaa ttcataatga aagcgagcgt
    21661 gctgcaggtc cttatatcgc cgtcaattgt gagttatatg gtgatgcggc gctggcggaa
    21721 gaatttattg gtggcgatcg cacggacaat gaaaatggcc gtctgagtcg gctggaactg
    21781 gcacacggcg gcacgctgtt tcttgaaaag attgaatatc tggcggtgga gttacagtct
    21841 gctttgcttc aggttatcaa gcagggtgtt atcacgcgac tggatgcgcg gcgtttaata
    21901 ccagttgatg tcaaagtgat tgccacaacg accgcggacc tcgcaatgct ggtggaacaa
    21961 aatcgtttta gtcgccagct gtattacgcg ttgcatgcat ttgaaattac catcccgcct
    22021 ctgcgtatgc ggcgtggcag cattccggcg ctggtgaata acaaattacg cagtcttgaa
    22081 aaacgcttct ctacgcggct gaaaattgat gacgatgccc tcgctcgcct ggtttcctgt
    22141 gcatggcctg gcaacgattt tgaactttac agcgtcatcg agaatcttgc tctgagtagt
    22201 gataatgggc gcattcgcgt cagtgatttg ccggaacatc tgtttaccga gcaggcaaca
    22261 gatgatgtca gcgccacacg cctttccacc agtctgtcat ttgcggaagt tgaaaaagag
    22321 gcaattatta acgcagccca ggtcacaggc ggtcgcattc aggaaatgtc ggctttactt
    22381 gggatcggcc gcactacgct gtggcggaaa atgaagcaac atggcattga tgcagggcag
    22441 tttaagcgcc gggtatgaaa gacagagacg atttctgata catcagagtg atctatattt
    22501 cattccggcg cacgctaaca tttttcagca tcgtttaagg gctggtctat cccgcactta
    22561 aaagctgaag cgatatcctc cgttgacttg cttctgatca aagaggttac tctgcgtgta
    22621 atccgcttcg aggtagaatg tgtgctgttt gttatactgt gcactgactc ccacgccgtt
    22681 attccagcca ttacctttga aactgtactt ctcccgggag ttgttcaaca gatagtctgt
    22741 atcccctgaa aactcgcgga tagctccagt cttcacatac atattcagct ggctgttgcc
    22801 tgcggtgatg tcatatccca gtatcatgct ggcacgcccc agcagcgatt cgtagtgatt
    22861 cagatgtata ttgagaccat tactcgcctt catagccatc tcattctggt ggctgtatgt
    22921 gagctgggtt tgcggctcta tatagaaccc ataaccagta ggggtcaggt tgaacctctg
    22981 cccggcttcc agggagatgc tcaatccatt cgcagtgccg ttggcgttaa cgccgttgtt
    23041 ctgactgtcc agtacgtgaa aactattttt ctggcgcgat gcttttacaa cgagatcgct
    23101 gtaaaaaccg ttgtgtgcca tgtaactggc atacattccc atgtagtctg aacgtgcggt
    23161 accgtcgcct ccgctatagt ccggcgatgc atgtgttgag ccaatataca gaccgacata
    23221 caacggcatt acatcagaga gacgtttatc cccaccaaac tggataccgc tgtaacccat
    23281 gtcaaagccg ctcagtttgc cactggcgaa ggagtccagg cttcccccat aactgcgcag
    23341 ccagatatta ccgtctttac tctgatttcg caggtcaccc attcgttgca tcagcgtacg
    23401 gttttcaaca tagtttaata agtaaccgac attgagataa ttgccgccag catctgcggt
    23461 cgtggtgggt ttaggtgtgg gagtggggtt aggcgtagga tcgggttcag gcgtaggatc
    23521 ggggttgact atgggaggct gagcgggagc cggtgttggt tcaggattag gagtcggttc
    23581 cggaactgtc ccggaagcgt aaagctccca gttagtgcca tttttacgca catcgtacag
    23641 atatcccccc aactcaacct gagaagacgc gctgaacgag gccgcaccgt cagtggtttt
    23701 taccactgtc agaacttcat ttcccgtagt ggcctcgctg ccctggttgc ggatagccaa
    23761 tacgtgatta ccagcactac ttccgctgat attcaataaa tcccctttat tattaacgcc
    23821 attaccctcg ccaacaacat cagcacgcat aataaaggta ctgttaccgc tcaggttctc
    23881 tacgtttaat gtggcaaatg tgccggcagt tgacccgtgg ctggcaaaat cgacagttga
    23941 atggctcagc gccagcgtgt cgagattaga attacttgtt acgttccaga cgctgttatt
    24001 cattgcaacg tccagtttcc cgccattgac attatcgctg agggaggaac ctgtccaaac
    24061 cgaccctggg tgcatatcta gattgattag cccacctttg gaaagaacgc taccgttgat
    24121 aagcatacga cccgaggcgt tgatgcggct ggcggcataa ccatcgtcat gttgcgttgc
    24181 aatggccatc tggtcgggtg tactcatatc aatgaccaga tcgctcgtga ggtcgatctg
    24241 gctgttggtc atggcataaa ttcccctggc tcctgccgcg ccggtgatag ccaaactgtc
    24301 tccggttatt ctaccgccgc tgagcgccca caaacccagc gccagactgc cattacgatc
    24361 aactgtaata tcggtatttt gcatgttgac aacagccgtt gccgtctggg ccgaggcacc
    24421 ataagaaccg ccggaaaaga tgctgtttcg ttgcgctgcc gtgccagaaa aattgattgt
    24481 cgcgtctgaa ccactggtga cgaggccacc gccctgcgcg gaagaaatat ggctatctgc
    24541 accgatagtg gttgtaccac cacgaacttc gacgccattg gccgcggctc cagttacatc
    24601 aacagtgagt gcattcgcgc taacctggcc aaagctccag aggccgtgtg cattatcgcc
    24661 attggtttta atggaactgt ttgttccgag atcgacaaca gagtttttct gtacgtttat
    24721 ccccatggcg ctgtagccct gaacatcgat tgtcaggtct gtagccgtaa aacgcgcagc
    24781 accattggcg ttattgccgt tgagaccacc gatataaaca cctgtacttc catcggtcgt
    24841 gattttactt ccgcttccaa gatcgacact ggtgccatag tcattgatgg ttaggcctat
    24901 accgttcgag ttttcaatgg tgaattgagt ggctgttagt gttgagctat gcccaataat
    24961 gatgccgtca tcgttactct taatggtgct gcctgtgcct aagtcagcat gggtatagtc
    25021 accaattaag ttaatgccga tggcagaggt ttgaccaaca acatctactg tgagtcggtt
    25081 agctgtcagg gagctgtttt tgccctgaat aatgattcct tttgcatatc cagaggcgtc
    25141 gttgacattg acggtgacat cattacccag gttgatattg ccaggcgtgt ttcctgctgg
    25201 ggtcattaca ccatagaggt ttcctgatgg atcggcggta tctcctgtaa ttctgtcacc
    25261 ttcattaata gtaatattgg tgccatattg acttcctgta tcagcagcta tcagcagggg
    25321 ggaaaagata attgcattca aaccaatagc gccaaatttt atcagttgaa ttgagggcga
    25381 acggattttt aattcagcga gacgatctgc tttggtattc ccattgtgtt gtttgatgcc
    25441 cataccactc ctatatagta cccagactat gggtctatgg attttgtcat tttgtttaaa
    25501 tcaatgaata atatcctctt atcatcatga tatttgtatg gtgcaatcat gatgagcttc
    25561 tcagagaata attctctatt accgggcaaa atcaggaaca caggaattaa tatatattta
    25621 acatccccct tacaaaggtg agtttatatc gagttattcc ctaagttgtc gttgcttatt
    25681 ccaattcgtt actataaata tggcaaaaat attacaacaa acagcggtta aaaaatttgg
    25741 tgaagatttt ttgtaataat cctaattagt tatgggaatt tcgcttgatg tatctgcata
    25801 acaaaaacaa tatgcgccat ttttgcatta ctaataagaa aaagcaaaaa acatcgatat
    25861 tcatttttgg tcaatgggtt ttctggccta tcgttgttat tgtgctactg gttatcattt
    25921 tcatcttgcc aggatcgcac gtaatcacgt tattcgggga ctttatctgc gggatattac
    25981 ccttcaggta agtccttttc tcgccagcat attggagcgt gatcaatatt gatcagctgt
    26041 gaagggggtg atgtgtagtg gaccaaaact tacgttatat cactactaat gagatatccg
    26101 tcatctgaca ctgatgtctt taattattct cgtggttcac tgtaggcaat aagcacaaaa
    26161 gtgtaggatg ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt
    26221 ggattatata tacttttgcg ctcttcatga gatttagcga aacctcatga gacaacaaat
    26281 taattagacg ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc
    26341 ttctgcacgc attttgcctg cttctttcgc gccttgttca cctttgtaag tgatgaaatc
    26401 ttcaaacgag atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc
    26461 cgctgcctgc ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc
    26521 agcggtgaag taagtttgca ggttcagcag cttataaccg gcacggatca cacggttcag
    26581 gcccggctct tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc
    26641 ggcaatgtct gcttcaacag cagcacaaac cggaaccaca acagaacctt ctttcgccgc
    26701 gatttcacgc acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc
    26761 gatgtacatt gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc
    26821 agcgcttaaa tccagcgcgc gcagcatacc tgcattttcc aactggggca ggcatttttc
    26881 caggaccgcc agctcagctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg
    26941 atgaatcgca cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt tgataacttc
    27001 aatatcgtca gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca
    27061 gcgaacaacg tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag
    27121 accttcgcct ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt
    27181 gggaagcgta cgttgcggtt ttacgatttc agccagttga tccaggcgag ggtcaggcat
    27241 tggtacgacg cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc
    27301 ggctttggtc agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc
    27361 gcatttgaat cccatgattt aaatcacctt aatatcttaa taatccacct gttattgcta
    27421 acagattgca gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg
    27481 cagacaacga cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca
    27541 tctgtaaacc acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc
    27601 tgttcactaa caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgcccgga
    27661 tgaccgattc cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg
    27721 tctttcagtc cattgtgacc accatggcca ccgcccaatt taaatttggc gacgccagga
    27781 ggcagatcca gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg
    27841 gccatcgcag caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg
    27901 acatcttcgc ctccaagagt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc
    27961 agcggagcgc gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc
    28021 gttgcggcgt attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt
    28081 ttttgtcctg agtgtgtaca taactggcgc gtagtttact ggttgcggcc ccgcttgaca
    28141 aaaaactgcg tatcaaatgc agataacgta ataattgcat gagtggacta ttagaaagtc
    28201 aaggtgttca ggcgtttatt tgtaaagttt tgttgaaata agggttgtaa ttgtgatcac
    28261 gcccgcacat aacccacagg gtgttgtcta tactttacac ataaggaaga ggggtattcc
    28321 ctgttacaac ccagaaagtt ccggaggtga catatgaaac gcaaaaacgc ttcgttactc
    28381 ggtaacgtgc tcatggggtt gggtctggtg gtaatggtgg tcggcgtggg gtattcaatc
    28441 ctcaaccagt taccacagtt taatatgccc cagtatttcg cacatggtgc agtgctaagt
    28501 attttcgtcg gtgccattct ctggctggcg ggtgcccgtg ttggcgggca tgaacaggtg
    28561 tgcgaccgtt actggtgggt tcgccactat gacaaacgtt gccgccgtag cgataatcgc
    28621 cgtcatagct aacaaataat gcagtttgct gaccagtcag tttgcgctga ctggtcaatc
    28681 tcgtacttat aaatccgcca tcgccgcgcg acgattcggg aagaacgcca ggcgtcccgg
    28741 gatcggttga atgccagcgc gagccatagt gcgcagtggc tggaattcca cgttgcacac
    28801 gcgcagttca catccctcgg gcagacgctt cacaaaacgc tggaacgcat caagaccacc
    28861 agcatcaagt accggaaccg catcccactt cagaatcaca atccgtttgc cttcaagacg
    28921 tgactccagg tccgtgaaca agccttcagc agcagcaaaa aacagcgggc caataacgcg
    28981 cagaaccagg acatcgtctg gaacatctac gactaccggt gccaggcgag tcatacgtgc
    29041 gatacgacgc ataaacagca gcgatgccag cacgatcccc acgctgatgg caataaccat
    29101 atcaaacagc acggtcagcg acatgcacag cagcatgaca atgatgtcat ctttcggcgc
    29161 atgacgcagc aagtcgacca ctttgtgcgc ttcactcatg ttccacgcca ccatcaacag
    29221 cagggcagcc atggcggaaa gcggcagcca ggagagcagc ggtgccagta ccagcagggc
    29281 aagaataacc agaatagagt ggatcaccgc cgagataggg gaagttgccc cggcacggac
    29341 gttagcggca gaacgcgcga tggcagctgt agcggtaatg ccaccaaaga acggagcgat
    29401 gatattcccc agtccctgtc caaccagttc gctattcgcc ttgtgtttcg tcccggtcat
    29461 accatccagc accacggcgc agagcagaga ttcgattgcg ccgagcattg ccattgagaa
    29521 tgccgcaggc agcagtgtgc gaatagaatc ccaggttagc gtgaattctg aattaggcag
    29581 atcccacggc agcaccagtt gcggcagcag ttgcggaata ccgttaccct gagaaccatc
    29641 ggccaggacg tagtggaatt gcgaaccgat ggtagcaaca tgtccgccga gcaggttaac
    29701 aatccccatc accgcgcaac cagccagcaa ggccggaagg tgaccgggta aacgaatgcc
    29761 cagacgcggc cagaaaacaa gaatacctag cgtcacaatg ccaatggcag catcacccac
    29821 attaatggtc ggcagcgcca taaataatgc gccgactttt tgtagataat gttccgggac
    29881 atgggccatt tgcagaccga gaaaatcttt aatctgcatg gtaccgatgg tgatcccgat
    29941 acccgaggtg aaacctaagg tgacggaaac cggaatatac tcaatcaggc gaccaaagcg
    30001 tgccagaccc atcagaatca aaaaaatccc cgacagcaag gtcgcaacca gcagtcctgc
    30061 cagtccaaac tgttgcgaca cgggatagag aattaccaca aatgccgcag tcggaccgga
    30121 aacgctaaag cgtgacccac ccgtcagagc aatgacaatc cccgcaacag ctgcggtata
    30181 taaaccgtac tggggtgcca caccactacc aatagccaac gccatcgcca gcgggatagc
    30241 aataatcccg acggttatcc cggcaatcag gtcacgggta aaccgtgcgg cagtatattt
    30301 ttctttccag caagcgtcga tcagagcgcg gaaaggcatc acatgtgagg aaaatatttt
    30361 gttcacaata atgtttcatc cgtgagcgca tcatctgtca actaaatggc aggtgaagga
    30421 ggcataggtc atacaaatgg atattacaga caaaaaaacc cgccgcagcg ggtctttgag
    30481 ccgggttcga ttagtgttcg aacatggcag agatcgattc ttcgttgctg atacgacgaa
    30541 tcgcttcggc cagcatacct gacagggtca gagtacgcac gttcggcagt gatttgattt
    30601 catcgctcag cggaatggta tcgcagacaa cgacttcatc aattacagag ttacgcaggt
    30661 tattcgccgc gttgccagag aagatcgggt gagtcgcgta cgcaaataca cgtttagcac
    30721 cacgttcttt cagagcttca gcagctttac acagcgtacc gccagtgtcg atcatatcat
    30781 cgaccagtac gcagtcacga cctgcaacgt caccgatgat atgcatcacc tgggaaacgt
    30841 tcgcacgcgg acgacgtttg tcgatgattg ccatatcggt atcgttcagc agcttagcga
    30901 tagcgcgggc acgcacaacg ccgccgatgt ccggagaaac cacaattggg ttatccagat
    30961 tcagctgcag catgtcttcc agcaggatcg ggctaccaaa tacgttatca accggaacgt
    31021 cgaagaaacc ctgaatctgt tcagcgtgca gatccactgt cagcacacgg tcaacaccga
    31081 cgctggagag gaagtctgca accactttcg cagtgattgg tacacgagcg gaacggacgc
    31141 gacggtcctg gcgcgcatag ccaaagtagg ggataacagc ggtgatacga cctgcggaag
    31201 cacgacgcag ggcatcaacc ataacgacta attccatcag gttgtcgtta gtaggggcac
    31261 aagtggactg gatgatgaaa atatcaccac cgcgtacatt ttcgttgatt tgtacactga
    31321 cttcgccgtc gctaaagcga cctacagcgg cgtcgccgag tgaagtgtac aggcggttgg
    31381 caatacgttg tgctagttcc ggggtggcgt taccagcaaa aagcttcata tcaggcacga
    31441 gaagaacctc aggcatgcgt ccattggtgg aaagaatctg ccgaaaactg tgcgggccag
    31501 gcatgatcct ttccaggcgg tgtattaaag agcgcgatgc aacgtctgga acaaggtgac
    31561 gttgtcaccg aaactcagct tgcccggctt aaagcatggc tctgtgcaat ggggaaagat
    31621 taacgccttt cgccacaaag ccattgagcc attccggggc ttgctctagc acctggcggg
    31681 cttcagactc tgtatcaaat tcagcaaaga cacaggcccc tgtcccagtc aggcgcgacg
    31741 gggcgtattc taacagccag gaaagcaccg catcaacctc gcgaaaacgt tttcttgcga
    31801 taacctcgca atcattgctg aattcacatt ttagcaacgt ttctattgac ctttttggcg
    31861 tattgcgcgg gagttcagga tctttaaaaa tcaccggagt cggaatactt acactagggt
    31921 gcgccaccag ataccacttc tctggcggat ccaccggcgt tagtatttca ccaacgcctt
    31981 cggcaaacgc ggcatgcccc cgaacaaaga caggaacatc tgcgcccagc gtcagcccca
    32041 tttccgccag ctcatccatg cttagcccgc attgccagag atgatttaat gccaccagga
    32101 ccgtcgcggc attggatgaa ccaccgccga gaccgccgcc catcggcaaa cgcttgtcaa
    32161 tgctgatatt cgcaccgctt cccgtcggaa gacgcccgct gtctgccgca gttttcatca
    32221 acaatcgcgc tgcgcgaacg atcaggttat cttcatgttc cacgccttca acgggcgtta
    32281 acagacgaat atccccatcg tcacgaagct caatgctgat ggtgtcgccg taatcaagaa
    32341 actgaaacag cgtttgcagc gtgtggtaac catccgcacg ctgaccggta atgtataaaa
    32401 acagattaag ttttgccgga gagggccact gtgtccgcat tatttcacta tccagttatc
    32461 catttttaac ttgatgcgtt gaccaccgtc ggtgagttcc atattggctg gcatcgcagg
    32521 ttgcgttttg gtgtcataac caccataaac aaccttccag tttttgccat tctggctgta
    32581 ggtaatttcg ctcaggcggt actggtcgtc cagtttgtag tcggttgcat cacccggtaa
    32641 acctaaaatc cactggcgca agctgttgag cggaattggc attccggtca atttgccaat
    32701 catctcttcg gcgtcatcgg cggtataacg ctgaccttta ttgtcgacta actgcacgtt
    32761 acccggttga gcattcagct ccagttccgt gctgcccaat gggttagtga gcagcagacg
    32821 gtagcgatcc tggccggttt gctgccagaa aaagcgggcg tacacttttt gttggtcaga
    32881 aatataagcg aacgcgccgc gagtctgata ctgattaaga ttgcgcacgt cttgctgatg
    32941 ctgacgccat tgtggcgaat ccgggctttt gccaggacct ttgggcgtgg taacggaaca
    33001 ggcagtgagc acaagagcag ccagcggtag caggcggata agacgaaaat cgggcagggg
    33061 catagtgatg acaagtcctt gagatacgtt gcagttataa cccttaatgc tagcgttacc
    33121 gtccgctatc gtctatgttc aagttgtctt aattgccaga atctaacggc tttcggcaat
    33181 tactccaaaa gggggcgctc tcttttattg atcttacgca tcctgtatga tgcaagcaga
    33241 ctaaccctat caacgttggt attatttccc gcagacatga cccttttagc actcggtatc
    33301 aaccataaaa cggcacctgt atcgctgcga gaacgtgtat cgttttcgcc ggataagctc
    33361 gatcaggcgc ttgacagcct gcttgcgcag ccgatggtgc agggcggcgt ggtgctgtcg
    33421 acgtgcaacc gcacggaact ttatcttagc gttgaagagc aggataacct gcaagaggcg
    33481 ttaatcctct ggctttgcga ttatcacaat cttaatgaag aagatctgcg taaaagcctc
    33541 tactggcatc aggataacga cgcggttagc catttaatgc gtgttgccag cggcctagat
    33601 tcactggttc ttggggagcc gcagatcctc ggtcaggtta aaaaagcgtt tgccgattcg
    33661 caaaaaggtc atatgaaggc cagcgaactg gaacgcatgt tccagaaatc tttctctgta
    33721 gcgaaacgcg ttcgcactga aacagatatc ggtgccagtg ctgtgtctgt cgcttttgcg
    33781 gcttgtacgc tggcgcggca gatctttgaa tcgctctcta cggtcacagt gttgctggta
    33841 ggcgcgggcg aaaccatcga gctggtagcg cgtcatctgc gcgaacataa agtacagaag
    33901 atgattatcg ccaaccgcac tcgcgaacgt gcccaaattc tggcagatga agttggcgcg
    33961 gaagtgattg ccctgagtga gatcgacgaa cgtctgcgcg aagccgatat catcatcagt
    34021 tccaccgcca gcccgttacc gattatcggg aaaggcatgg tggagcgcgc attaaaaagc
    34081 cgtcgcaacc aaccaatgct gttggtggat attgccgttc cgcgcgatgt tgagccggaa
    34141 gttggcaaac tggcgaatgc ttatctttat agtgtggacg atctgcaaag catcatttcg
    34201 cacaacctgg cgcagcgtaa agccgcagcg gttgaggcgg aaactattgt cgctcaggaa
    34261 accagcgaat ttatggcgtg gctgcgagca caaagcgcca gcgaaaccat tcgcgagtat
    34321 cgcagccagg cagagcacgt tcgcgatgag ttaaccgcca aagcgttagc ggcccttgag
    34381 cagggcggcg acgcgcaagc cattatgcag gatctggcat ggaaactgac taaccgcttg
    34441 atccatgcgc caacgaaatc acttcaacag gccgcccgtg acggggataa cgaacgcctg
    34501 aatattctgc gcgacagcct cgggctggag tagcagtaca tcattttctt tttttacagg
    34561 gtgcatttac gcctatgaag ccttctatcg ttgccaaact ggaagccctg catgaacgcc
    34621 atgaagaagt tcaggcgttg ctgggtgacg cgcaaactat cgccgaccag gaacgttttc
    34681 gcgcattatc acgcgaatat gcgcagttaa gtgatgtttc gcgctgtttt accgactggc
    34741 aacaggttca ggaagatatc gaaaccgcac agatgatgct cgatgatcct gaaatgcgtg
    34801 agatggcgca ggatgaactg cgcgaagcta aagaaaaaag cgagcaactg gaacagcaat
    34861 tacaggttct gttactgcca aaagatcctg atgacgaacg taacgccttc ctcgaagtcc
    34921 gagccggaac cggcggcgac gaagcggcgc tgttcgcagg cgatctgttc cgtatgtaca
    34981 gccgttacgc cgaagcccgc cgctggcggg tagaaatcat gagcgccagc gagggtgaac
    35041 atggtggtta taaagagatc atcgccaaaa ttagcggtga tggtgtgtat ggtcgtctga
    35101 aatttgaatc tggcggtcat cgcgtgcagc gtgttcctgc tacggaatcg cagggtcgta
    35161 ttcatacttc tgcttgtacc gttgcggtaa tgccagaact gcctgacgca gaactgccgg
    35221 acatcaaccc agcagattta cgcattgata ctttccgctc gtcaggggcg ggtggtcagc
    35281 acgttaacac caccgattcg gcaattcgta ttactcactt gccgaccggg attgttgttg
    35341 aatgtcagga tgaacgttca caacataaaa acaaagcgaa ggcgctttca gtgctcggtg
    35401 ctcgcatcca cgctgctgaa atggcaaaac gccaacaggc cgaagcgtct acccgtcgta
    35461 acctgctggg gagtggcgat cgcagcgacc gtaaccgtac ttacaatttc ccgcaggggc
    35521 gcgttaccga tcaccgcatc aacctgacgc tctaccgcct ggatgaagtg atggaaggta
    35581 agctggatat gctgattgaa ccgattatcc aggaacatca ggccgaccaa ctggcggcgt
    35641 tgtccgagca ggaataatgg aatatcaaca ctggttacgt gaagcaataa gccaacttca
    35701 ggcgagcgaa agcccgcggc gtgatgctga aatcctgctg gagcatgtta ccggcagagg
    35761 gcgtactttt attctcgcct ttggtgaaac gcagctgact gacgaacaat gtcagcaact
    35821 tgatgcgcta ctgacacgtc gtcgcgatgg tgaacccatt gctcatttaa ccggggtgcg
    35881 agaattctgg tcgttgccgt tatttgtttc gccagcgacc ttaattccgc gcccggatac
    35941 ggagtgtctg gtggagcagg cactggcgcg gttgcctgaa caaccttgcc gtattcttga
    36001 tctcgggacg ggtaccgggg cgattgcgct tgcgctggct agcgagcgcc cggactgcga
    36061 aattatcgct gtagatcgta tgcctgatgc tgtctccctg gcacaacgta atgcccagca
    36121 tctggcgatc aaaaatatcc acattctgca aagcgactgg tttagcgcgc tagccgggca
    36181 gcagtttgcg atgattgtca gcaatccgcc gtatattgac gagcaggacc cacatcttca
    36241 acaaggcgat gtccgctttg agccgctcac tgcgctggtt gcggcagaca gtggaatggc
    36301 agacatcgtg catatcatcg aacagtcgcg taacgcgctg gtatccggcg gctttctgct
    36361 tctggaacat ggctggcagc agggcgaagc ggtgcgacaa gcatttatcc tcgcggggta
    36421 tcatgacgtc gaaacctgcc gtgactatgg tgataacgag cgcgtaacgc tcggccgcta
    36481 ttatcaatga caagtttttc tacactgctt agtgttcatc ttattagtat cgcgctttct
    36541 gttgggctat taaccttacg tttctggcta cgttatcaga agcatccaca ggcatttgct
    36601 cgctggacgc gcattgtgcc gccggttgtc gatacggtgt tattgttaag cggcattgcg
    36661 ttgatggcta aagcgcacat cctgccattt tccgggcagg cacagtggct gactgaaaag
    36721 ctgtttggag ttatcattta tatcgttttg ggttttattg cactcgatta tcgtcgtatg
    36781 cacagtcagc aggcgcgcat tattgccttc ccgctggcgt tggtggtgct gtacatcatc
    36841 attaaactcg ccaccacaaa agtaccgtta ctggggtaag tcatgagatc gttagctgat
    36901 ttcgaattta ataaagcgcc attgtgcgaa ggcatgatcc tggcttgcga agcaatccgc
    36961 cgcgattttc cctcgcaaga tgtttacgac gaactggagc gtctcgttag tctggcgaag
    37021 gaagaaatca gccagcttct gcctttagaa gagcagctgg aaaaactgat cgcgctgttt
    37081 tacggcgact ggggatttaa agcctcacgc ggcgtttatc gtctttccga tgcattatgg
    37141 ctggaccagg tgttaaagaa tcgacagggc agtgcggtat cattaggtgc ggttttatta
    37201 tgggtcgcga atcgtctcga tttgccgctg ctgccggtga ttttccctac gcagctgata
    37261 ttgcgcattg aatgtccgga tggcgaaatt tggctgatta atccttttaa cggtgaatcg
    37321 ttaagcgaac atatgctgga cgtatggtta aagggaaata tcagcccgtc ggcggaactg
    37381 ttttatgaag accttgatga agctgataac attgaggtaa tccgcaaatt gctggataca
    37441 ctcaaagcct cgttgatgga agaaaatcag atggagctgg cgttacgcac cagcgaagct
    37501 ttattacaat tcaaccctga agatccctat gaaattcgcg atcgcgggtt gatttatgtg
    37561 caactggatt gcgaacacgt tgcgttgaac gatttaagtt atttcgttga acagtgtccg
    37621 gaagacccga tcagcgaaat gatccgtgcg caaataaata acatcgcgca taaacatatt
    37681 gtgctgcatt aattaatcga cattttactc aagattaagg cgatcctatg aaacaaaaag
    37741 tggttagcat tggcgacatc aacgtagcaa atgacctgcc gttcgtactg tttggcggta
    37801 tgaacgtgct ggaatctcgc gatctggcga tgcgcatttg cgagcactac gtaactgtga
    37861 cccagaaact gggtatccct tacgtgttca aagcctcttt tgacaaagcc aaccgctcct
    37921 ccatccactc ttatcgtgga ccgggcctgg aagaagggat gaaaatcttc caggagttga
    37981 agcagacttt tggcgtgaaa attatcaccg acgttcacga accaagtcag gcacagcccg
    38041 ttgctgatgt cgtggatgtg attcagttgc cggcgtttct tgctcgccag actgacctgg
    38101 ttgaagccat ggcgaaaacc ggtgcggtaa ttaacgtcaa gaaaccacag tttgtcagcc
    38161 cgggacagat gggtaatatc gttgataaat tcaaagaagg cggcaacgaa aaagtgattc
    38221 tttgcgatcg tggtgctaac ttcggctatg acaacctggt tgtcgatatg ctgggcttca
    38281 gcattatgaa gaaagtgtct ggtaactcgc cggtgatttt cgacgtgacc cacgcactgc
    38341 aatgccgcga tccgtttggc gcagcttccg gtggtcgtcg tgctcaggtg gctgagctgg
    38401 cacgagccgg tatggcggta ggtctggcgg ggctgtttat tgaagcgcat ccggatccgg
    38461 aacatgcgaa atgtgatggt ccatccgcgc tgccgctggc taaactggaa ccgttcctca
    38521 agcagatgaa agcgattgat gatctggtga aaggtttcga agaactggat accagcaagt
    38581 aatctttttt gcttgaaaaa taaagtctta gcgttctgcg ttaagacttt tttcatgggt
    38641 gccagataca aaaaaggccg caggctgtta cccctgcggc cggtttcggg cgcatattgc
    38701 catcacggca gcctgacgcc cgttttcacc ttacttccgg ttacgccacc agccgacaat
    38761 cgctgcggta ataattcccg ccaggatcgg tgctgccagg tcgtgccaga aaatcatggc
    38821 aaactgcgcg agcgtcatat agccgccttg ttgtaatgac aacattttgc ggctattctt
    38881 gaattgttct ggttcaagat tagcccccgt tctgttgtca ggttaaacct ctcaacgtgc
    38941 gggggttttc tctttccagc aaccaatgcc accagggata aagcccccgc aacattgcgc
    39001 ctcaccggat aacgccggct tgatgtggat actacgtctc aattcatctt cacttcatcc
    39061 ctgaaatgtt tgcaatgaag agtgcattcc ggtttttcaa cagctgttac agtcgtttca
    39121 tgagggctct ggatgatgct tccagctcgg gttgccaatg tttactggcg gaagagataa
    39181 agacaaaaaa ggccgcaggc tgttacccct gcggccggtt tcgggcgcat attgccatca
    39241 cggcagcctg acgcccgttt tcgccttact tccggttacg ccaccagccg acaatcgctg
    39301 cggtaataat tcccgccagg atcg
//
LOCUS       DAZGTR010000031        37787 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGTR010000031 DAZGTR010000000
VERSION     DAZGTR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.031"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(199..945)
                     /gene="cutC"
                     /locus_tag="ACHLCY_001787"
     CDS             complement(199..945)
                     /gene="cutC"
                     /locus_tag="ACHLCY_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP0369064.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(959..1525)
                     /gene="yecM"
                     /locus_tag="ACHLCY_001788"
     CDS             complement(959..1525)
                     /gene="yecM"
                     /locus_tag="ACHLCY_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0369065.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1741..3474
                     /gene="argS"
                     /locus_tag="ACHLCY_001789"
     CDS             1741..3474
                     /gene="argS"
                     /locus_tag="ACHLCY_001789"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP0369066.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3516..3560)
                     /gene="yecV"
                     /locus_tag="ACHLCY_001790"
     CDS             complement(3516..3560)
                     /gene="yecV"
                     /locus_tag="ACHLCY_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFP0369067.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3651..4139
                     /gene="yecT"
                     /locus_tag="ACHLCY_001791"
     CDS             3651..4139
                     /gene="yecT"
                     /locus_tag="ACHLCY_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP0369068.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4259..4651)
                     /gene="flhe"
                     /locus_tag="ACHLCY_001792"
     CDS             complement(4259..4651)
                     /gene="flhe"
                     /locus_tag="ACHLCY_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP0369069.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTSVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4651..6729)
                     /gene="flhA"
                     /locus_tag="ACHLCY_001793"
     CDS             complement(4651..6729)
                     /gene="flhA"
                     /locus_tag="ACHLCY_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP0369070.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADCLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6722..7870)
                     /gene="flhB"
                     /locus_tag="ACHLCY_001794"
     CDS             complement(6722..7870)
                     /gene="flhB"
                     /locus_tag="ACHLCY_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP0369071.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8072..8716)
                     /gene="cheZ"
                     /locus_tag="ACHLCY_001795"
     CDS             complement(8072..8716)
                     /gene="cheZ"
                     /locus_tag="ACHLCY_001795"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP0369072.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8727..9116)
                     /gene="cheY"
                     /locus_tag="ACHLCY_001796"
     CDS             complement(8727..9116)
                     /gene="cheY"
                     /locus_tag="ACHLCY_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP0369073.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9131..10180)
                     /gene="cheB"
                     /locus_tag="ACHLCY_001797"
     CDS             complement(9131..10180)
                     /gene="cheB"
                     /locus_tag="ACHLCY_001797"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP0369074.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATTPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10183..11043)
                     /gene="cheR"
                     /locus_tag="ACHLCY_001798"
     CDS             complement(10183..11043)
                     /gene="cheR"
                     /locus_tag="ACHLCY_001798"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP0369075.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11062..12666)
                     /gene="tap"
                     /locus_tag="ACHLCY_001799"
     CDS             complement(11062..12666)
                     /gene="tap"
                     /locus_tag="ACHLCY_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP0369076.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNNDLEHQATWLESNQLSDFLTAPVQESQNAF
                     DVNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQP
                     LAIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHI
                     GIAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTA
                     QAGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVD
                     DIMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSR
                     VAVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12712..14373)
                     /gene="tar"
                     /locus_tag="ACHLCY_001800"
     CDS             complement(12712..14373)
                     /gene="tar"
                     /locus_tag="ACHLCY_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP0369077.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQAPNWETF"
     gene            complement(14518..15021)
                     /gene="cheW"
                     /locus_tag="ACHLCY_001801"
     CDS             complement(14518..15021)
                     /gene="cheW"
                     /locus_tag="ACHLCY_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP0369078.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15042..17006)
                     /gene="cheA"
                     /locus_tag="ACHLCY_001802"
     CDS             complement(15042..17006)
                     /gene="cheA"
                     /locus_tag="ACHLCY_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP0369079.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17011..17937)
                     /gene="motB"
                     /locus_tag="ACHLCY_001803"
     CDS             complement(17011..17937)
                     /gene="motB"
                     /locus_tag="ACHLCY_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP0369080.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDLISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17934..18821)
                     /gene="motA"
                     /locus_tag="ACHLCY_001804"
     CDS             complement(17934..18821)
                     /gene="motA"
                     /locus_tag="ACHLCY_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0369081.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18948..19526)
                     /gene="flhC"
                     /locus_tag="ACHLCY_001805"
     CDS             complement(18948..19526)
                     /gene="flhC"
                     /locus_tag="ACHLCY_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP0369082.1"
                     /translation="MSEKSIVQEAWDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19529..19879)
                     /gene="flhD"
                     /locus_tag="ACHLCY_001806"
     CDS             complement(19529..19879)
                     /gene="flhD"
                     /locus_tag="ACHLCY_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP0369083.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20659..21087
                     /gene="uspC"
                     /locus_tag="ACHLCY_001807"
     CDS             20659..21087
                     /gene="uspC"
                     /locus_tag="ACHLCY_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP0369084.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21094..22518)
                     /gene="otsA"
                     /locus_tag="ACHLCY_001808"
     CDS             complement(21094..22518)
                     /gene="otsA"
                     /locus_tag="ACHLCY_001808"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP0369085.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(22493..23293)
                     /gene="otsB"
                     /locus_tag="ACHLCY_001809"
     CDS             complement(22493..23293)
                     /gene="otsB"
                     /locus_tag="ACHLCY_001809"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP0369086.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGADLEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23460..24446)
                     /gene="araH"
                     /locus_tag="ACHLCY_001810"
     CDS             complement(23460..24446)
                     /gene="araH"
                     /locus_tag="ACHLCY_001810"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP0369087.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24461..25975)
                     /gene="araG"
                     /locus_tag="ACHLCY_001811"
     CDS             complement(24461..25975)
                     /gene="araG"
                     /locus_tag="ACHLCY_001811"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP0369088.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(26045..27034)
                     /gene="araF"
                     /locus_tag="ACHLCY_001812"
     CDS             complement(26045..27034)
                     /gene="araF"
                     /locus_tag="ACHLCY_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP0369089.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            27831..28334
                     /gene="ftnB"
                     /locus_tag="ACHLCY_001813"
     CDS             27831..28334
                     /gene="ftnB"
                     /locus_tag="ACHLCY_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP0369090.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKSMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(28413..28664)
                     /gene="yecJ"
                     /locus_tag="ACHLCY_001814"
     CDS             complement(28413..28664)
                     /gene="yecJ"
                     /locus_tag="ACHLCY_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP0369091.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(28779..28865)
                     /gene="azuC"
                     /locus_tag="ACHLCY_001815"
     CDS             complement(28779..28865)
                     /gene="azuC"
                     /locus_tag="ACHLCY_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP0369092.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            29128..29451
                     /gene="yecR"
                     /locus_tag="ACHLCY_001816"
     CDS             29128..29451
                     /gene="yecR"
                     /locus_tag="ACHLCY_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP0369093.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            29622..30119
                     /gene="ftnA"
                     /locus_tag="ACHLCY_001817"
     CDS             29622..30119
                     /gene="ftnA"
                     /locus_tag="ACHLCY_001817"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP0369094.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(30157..30396)
                     /gene="yecH"
                     /locus_tag="ACHLCY_001818"
     CDS             complement(30157..30396)
                     /gene="yecH"
                     /locus_tag="ACHLCY_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP0369095.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            30587..31798
                     /gene="tyrP"
                     /locus_tag="ACHLCY_001819"
     CDS             30587..31798
                     /gene="tyrP"
                     /locus_tag="ACHLCY_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP0369096.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     IALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(31860..32525)
                     /gene="yecA"
                     /locus_tag="ACHLCY_001820"
     CDS             complement(31860..32525)
                     /gene="yecA"
                     /locus_tag="ACHLCY_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP0369097.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(32721..32807)
                     /locus_tag="ACHLCY_001821"
     tRNA            complement(32721..32807)
                     /locus_tag="ACHLCY_001821"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32771..32773),aa:Leu,seq:taa)
     gene            complement(32820..32893)
                     /locus_tag="ACHLCY_001822"
     tRNA            complement(32820..32893)
                     /locus_tag="ACHLCY_001822"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32859..32861),aa:Cys,seq:gca)
     gene            complement(32948..33023)
                     /locus_tag="ACHLCY_001823"
     tRNA            complement(32948..33023)
                     /locus_tag="ACHLCY_001823"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32988..32990),aa:Gly,seq:gcc)
     gene            complement(33175..33723)
                     /gene="pgsA"
                     /locus_tag="ACHLCY_001824"
     CDS             complement(33175..33723)
                     /gene="pgsA"
                     /locus_tag="ACHLCY_001824"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP0369098.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(33780..35612)
                     /gene="uvrC"
                     /locus_tag="ACHLCY_001825"
     CDS             complement(33780..35612)
                     /gene="uvrC"
                     /locus_tag="ACHLCY_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP0369099.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKVGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(35609..36265)
                     /gene="uvrY"
                     /locus_tag="ACHLCY_001826"
     CDS             complement(35609..36265)
                     /gene="uvrY"
                     /locus_tag="ACHLCY_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP0369100.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEVSCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            36407..36478
                     /locus_tag="ACHLCY_001827"
                     /pseudo
     CDS             36407..36478
                     /locus_tag="ACHLCY_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            36561..36737
                     /gene="yecU"
                     /locus_tag="ACHLCY_001828"
     CDS             36561..36737
                     /gene="yecU"
                     /locus_tag="ACHLCY_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP0369101.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            36724..36948
                     /gene="yecF"
                     /locus_tag="ACHLCY_001829"
     CDS             36724..36948
                     /gene="yecF"
                     /locus_tag="ACHLCY_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP0369102.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(37015..37737)
                     /gene="sdiA"
                     /locus_tag="ACHLCY_001830"
     CDS             complement(37015..37737)
                     /gene="sdiA"
                     /locus_tag="ACHLCY_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP0369103.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         9352 a         9989 c         8944 g         9502 t
ORIGIN      
        1 agcaatttaa atcccataat caatgtgaat ataaaaaata ttattagcac aacgctatgt
       61 tcactgtcac ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata
      121 aaaaaaggcc cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac
      181 atgatgcaac ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc
      241 aaccgccgcc ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat
      301 ggacaatcct tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg
      361 gacttccagc actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc
      421 ggccataatg attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg
      481 cagtgcgtct gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt
      541 attgagtgta tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag
      601 cggaccggca gcagccatta ttttttccat tcgtggcata tcgacattcc cgtcaacatc
      661 gagaacgccc gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag
      721 aatggcggca aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg
      781 atgcacaggg atcgtcaccc gctggcgcac ggatttcagt acccccaacg acggcgttaa
      841 gcccccctct tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt
      901 tagtgcacat tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt
      961 acgctgactg ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg
     1021 ttttgccatc ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg
     1081 aactggtttt aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg
     1141 cacgggcatt cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt
     1201 cgtgtgggta acgtttttcc ccgggccacg gtagctccac aatagaaaac tgccagtgcg
     1261 caacctgtac cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatatttt
     1321 ctgacaaaag ctcgccacac tgttcaaacc cgcgacgcca gcgttcagca gtggcgtttt
     1381 gatggcaacg caaagaaatg tgatcggcag tcagcggagt gatattcaac cccagacgga
     1441 gggaaagttc atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct
     1501 cgtcgataga ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt
     1561 accctgttgc cctgcgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa
     1621 aacggcaata cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc
     1681 cctaatttct ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg
     1741 gtgaatattc aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg
     1801 cctgcggatt gcgaaccgca ggttcgtcag tcagcaaaag ttcagttcgg cgactatcag
     1861 gctaacggca tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag
     1921 caggtgctga ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca
     1981 ggctttatca acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg
     2041 gcgtccgatc gtctcggtgt ttctacgcca gaaaaacaga ccattgtggt tgactactct
     2101 gcgccaaacg tggcgaaaga gatgcatgtc ggtcacctgc gctctaccat tattggtgac
     2161 gcagcagtgc gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc
     2221 gactggggca ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac
     2281 gccggtgaaa tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac
     2341 gacgaagatg aagagttcgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt
     2401 gacgaatatt tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccag
     2461 atcacttacg atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc
     2521 tacaacccaa tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa
     2581 agcgaagggg cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg
     2641 ggcgtgatca ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt
     2701 gcgaaatatc gttatgaatc actgcatgcc gatcgcgtgc tgtattacat cgactcccgt
     2761 cagcatcaac acctaatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa
     2821 tccgtaccgc tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc
     2881 aaaacccgcg cgggtggtac agtgaaactg gccgatctgc tggatgaagc cctggaacgt
     2941 gcacgccgtc tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg
     3001 gctaacgcgg ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg
     3061 gactacatct tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg
     3121 cagtatgctt acacgcgtgt attgtccgtg ttccgtaagg cagaaattaa cgaagagcaa
     3181 ctggctgcag ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg
     3241 ctgcagtttg aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtgatgtgt
     3301 gcttacctgt acgatctggc tggtctgttc tctggcttct acgagcactg cccgatcctc
     3361 agcgcagaaa acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag
     3421 acgctaaagc tgggtctgga tacgctgggt attgagacag tagagcgtat gtaatcgatt
     3481 tttcgtgaga gtaaagcctg atcagggtta cctgatcagg cttttttatt gccatctaaa
     3541 tgtattctga aaatggacat gccattgttt tctgactgtt ggataagagg ccagaagcgt
     3601 aatatccggc cccagggaaa cgataacggt tgaatttaag gaataccgca gtgtttaaat
     3661 ttcttgtatt aacattaggc attatctctt gccaggctta cgcagaagat acggttatag
     3721 taaacgacca tgacatttca gccatcaaag attgttggca aaaaaattca gatgatgata
     3781 ctgacattaa cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatgcgcaat
     3841 taaataaagc ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgtaa
     3901 aaaaacctga taccgaacaa cttaacttgc ttaaaaaatc acagcgagcc tggctggatt
     3961 ttagggacaa agaatgtgaa ttaatccttt caaatgagga cgttcaggat ttaagtgacc
     4021 cttattctga atcagaatgg ctctcatgta tgatcataca gaccaatacg cgaactcgcc
     4081 agttgcagct ataccgtaac tctgaagatt tttatccaag ccctttgaca agaggataat
     4141 tcacatcttt ttggcatgtt ttgttgcaag ctattcctga taaataattg caacaagaca
     4201 tcgagccttt ttcactgagt tattaaacat actcgcgagc gcgtaatttt tttgtccttc
     4261 agcgataatt cacaatcact tcattacgtt gtacctttag cggtggaatt aaccgcccac
     4321 cgcctggcac ttcccagata aatcgcaacg gttctgctgc cgctataccg gaaaaggcca
     4381 cggaggttcc gctctgcccc tctaattcga cacaacgaga ttgcgaacac aagcgaaccc
     4441 gcagtcctga aggtgtcggg ccgataagct gataacgcca cgctaccagc gtcatcaatc
     4501 ctgaagcagg ttgtcgcgta gaaagaggcg cagacgacat cgactcaccg cgatgattta
     4561 gcgtaattcc tacactactt gcctgccaca tcccctcccc ggcggcttgc accagcagcg
     4621 gaaacaataa tattgctaat aaggttctca ttatttgccg ccaattgtcg ccgtcatgcg
     4681 gatatgtcgg ttatcagaca gttccagatt cgacaggacc actaactgcg gcaagctgcg
     4741 gcgcaggaag cgagacaata atggtcgcag cgcgtggttc accaacaata ctggcggcgc
     4801 acccagcatc tcctgacggg atagcgcttc ctgagtttgc gccagtaaac aatccgccag
     4861 ccctggctcc agtcctcccc cgccctgcag cgcctgtagt aacaaacgtt ccagcggtgt
     4921 atcgaggcca ataacatgga cttcatcttt gccaggaaac cactgctggg taatcgcccg
     4981 tcccaacgcc acgcgcacga cggcggttaa ttcatgtgga tcgctttgga tgggcgcatg
     5041 ttccgccagc gtttcgagaa tggtgcgcat atcgcgaatc ggtacttttt catcgaggag
     5101 attttgcagc actttatgca gtgtggtgag cgtgacgacg ccaggaacga gatcttccgt
     5161 cagctttggc atctcctggg cgacgcgatc caacagctgt tgcgcctcct gacgaccaaa
     5221 cagctctgcg gcatgctggc taatgaggtg gttaagatgc gttgctacca ccgtgctggc
     5281 ctcaaccact gtgtacccct gaatctgcgc ctgttctttt agcgcacttt caatccagat
     5341 agcattcagg ccaaatgccg gatcgacggt cgcctcacca ggtaacgtcc cggcagcggt
     5401 tccagggtta atcgccagcc agcgccccgg ataagcatca ccactgccaa tctccacgcc
     5461 tttcatcaaa atgcgatagc gggcaggttg cagatccata ttgtcgcgaa tgtgcaccac
     5521 tggcggcaga aatcccatct cctgggcaaa tttcttgcga atactgcgta tacggcccaa
     5581 caactcacca tcctgctgga aatcgaccat cgggatcagt cgataaccca cttccattcc
     5641 cagagaatct tccagttgta catcgttcca cgtcgcttcg acaacggtat tattctctgc
     5701 catttttacc ggtttgggtt cgacaggcgc tttttgttcg cgtccgcgta tccaccaggc
     5761 cagccccagc aatccggcag tgaacagcaa aaataccagg ttcggcattc caggcaccag
     5821 gccgagtaaa ccgagcacgg cggcgcttaa caacataacg cttgggttac tgaaaagctg
     5881 attcaccatc tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga tgacccccgc
     5941 ggcggtagaa atcaccagcg ccggaatttg tgccaccaga ccgtcgccaa tggtcaatag
     6001 cgtataactt tccgccgcgt gtcccatgct catgccatgt tgcagcacgc cgaccagcaa
     6061 cccgccgaca acgttaatga ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa
     6121 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     6181 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     6241 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgcgatac gcccggcacc
     6301 tttggtaatg accataaagt tgatgatcac gagaatgaca aacactacga taccgatagc
     6361 gaaattgcca ccaacgagga agtgaccgaa tgcctcgacc accttcccag ccgccgccgc
     6421 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     6481 caacgtagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     6541 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     6601 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     6661 ggcaaggatc tgccattgtg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     6721 attactcatg ggtcggtttc tcgttaataa aatccagggc ttccggcacc ggaagatgag
     6781 taggttgtac agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagacccagg
     6841 ccagcacttc cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccgcat
     6901 gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc
     6961 caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc
     7021 tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga
     7081 catccgcttt cggcacatcg gccatcatcc gacgccgtgc agcagctcgc tgcatctgac
     7141 ggatccgccc tttaacatga gggtcacctt cgctttgttt gaactcatca cgaatatcct
     7201 gccgtgacat acgcagcttt ttcaggtggc tgaatatttg gaaaaagacg tcaaatccca
     7261 ccattggaat gacaccaagc accaccagca gtgcgcatag ccctaccaaa tccatcgcat
     7321 tacccatggc ggtaatcgga gactcggcca tcaagcgcat catctgcggc cagtgatgcc
     7381 agagaaaaaa ccccgtcacg ctgccaacca ggatggtttt caaaattgct ttaagcaact
     7441 ccgcgccagt ctgagccgag aacatccgtt taatgcccgg tagcgggttg agtttggaaa
     7501 acttcggctg caaggatttg ccgctaaata ccagccctcc cagcatgacc ggagaaatga
     7561 gcgccaccag caccacgccg ctaatcagcg gcagcagcgc cagcatggct tctctgatca
     7621 gcagaataat ctgcccgagg atcagattcg ggtcattgat aatactgtga tcaaaatgca
     7681 gcccagcgga gagcatgccc gacaatcgac gggccagcga cacaccgcca aaccagataa
     7741 cactaacgcc caccagcaaa atcagcagtg aggtcagttc acgggaacgc gggatttgcc
     7801 cctcttcccg cgctttttct agtcggtgag gtgtgggggc ttctgttttg tcgtcgctct
     7861 cgtcagacac gtcgccaatc ctggaaagag tattaagcgt gaatgatgcc agagcgcaaa
     7921 gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt tagggctttt gctgccacac
     7981 atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc cttatcagac cgcctgatat
     8041 gacgtggtca cgccacatca ggcaatacaa atcaaaatcc aagactatcc aacaaatcgt
     8101 ccacctgatc ctgactggct accacaccgg ctttgctggt atcgacctga ggtccattaa
     8161 gcaaactctg gttttcacgt tttggacgcg actcctgttc cgggatgttt tccaacagca
     8221 ccatcagcaa ctggcgttcg atctcctgaa tgacatccat catccgctta atgacctgcc
     8281 cggtgagatc ctgaaaatcc tgcgccatca tgatttccag cagttgcgcg ttagtaaagc
     8341 tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc tgttaccagt tcacgggcgt
     8401 cggcaaggtc aatcggatcg gcaaaccagt catcccaacg ttgggttaac gcttttgctg
     8461 atttctccat ttgatcctga tgcggttgtg acgcctcaac actgttcagc gcccgctccg
     8521 cagcctgggc ggtcatctgc acaacatagt acaaacgatc gcgcgcatcg gggatggctt
     8581 ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg caaactgtcg cgcagcatac
     8641 gcgtcaggct gccgatgcgc gcaatgatat cgccagctga atgctcgtca gcaggtttga
     8701 ttgatggttg catcatagtc gcatcctcac atgcccagtt tctcaaagat tttgttgagt
     8761 ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat agccactggc ccccgcttgc
     8821 gccgcagcaa tgatgttctc tttcttcgct tctgcagtca ccattaacac tggcaatgcc
     8881 gacatcgcgc catccgcacg aattgttttc agcaattcca ggccatccat attgggcatg
     8941 ttccagtcgg agataacaaa tccataaccg cctgcctgca acttattgag agcgtcgacg
     9001 ccatcttccg cttcctcaac attattgaat cccagctctt tcagcaggtt acgcactatg
     9061 cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa gttctttatc cgccatttca
     9121 cactcctgat ttaaatacgt atcgcctgtc cggcactaat ttttgccaac atttgctggc
     9181 ttacctggct aagatcgacc acttcgcaga caccacccat attgatggcc tcgcgcggca
     9241 tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt ccatgccccc gcctgacgca
     9301 tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt caggatcacc ccaaccgcat
     9361 tacgccccgc ctgtttggcg acagaatgga acaacacatc taccgaaggc cgatgacggt
     9421 taaccgccgg gccatcgtga attttgattt ggtaatttgc gccactacgc gacagctcca
     9481 tatgccgatc gcccggcgca atataggcat gccccggcaa gacacgttct ccgtcttcgg
     9541 cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag cgggtgaaac
     9601 cgggcggcat atgttgggta attaacaatg ccgggctgga aagcggcaac ggttgcagta
     9661 cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt ttttcagaac
     9721 tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa tggcttatgt gctgcaaggc
     9781 tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc agcattcctt
     9841 cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc agcgcgcgca
     9901 gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat cacaacgggc attggacgca
     9961 aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca acatccagcg
    10021 tcagcacatc gggattgaat ttcttaatca agtcacgcgc gaccagcgga tcaggcgtgg
    10081 tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc tggcgcatca
    10141 gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct tacttagcgc
    10201 atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt tttcagagtg
    10261 acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca aaatctcctg
    10321 ctgggtattt tgatcgaagt agatcatgac gttacgacag aagatcgcat caaacggccc
    10381 cggcacggtg tactgtttcg ccagtagatt cagcggggca aaatcaacat agttcgccag
    10441 ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga aataccgttg
    10501 cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc ttctggcttt
    10561 ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg gcgcggtgcc
    10621 caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggtcg aagccgccgc
    10681 gctccatacg cgatactcgc cagaacggcg acgtgcgtga tccgcgagca gagggaaatg
    10741 gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa acgcctgcca
    10801 ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat ccgtcagtcc
    10861 cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat ggtcagccag
    10921 aacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaatgcg cgtcggaaag
    10981 cgccaggcgc tcggtcatct gtaacaataa agacgtttgc ccacagggca gagatgaagt
    11041 catagcgcct tctcaatcac ttcaggatac cactggcgca atttgtaact gcgccgactc
    11101 atgtcgtgct acttcatgtt cttcaagggt aaataccgcc acacgcgacg aaagatggtc
    11161 ggcctgattc gccagttgtt ccgttgccac cgccgcttct tctaccagcg aggcgttctg
    11221 ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc tgctcaatcc cccgctgttg
    11281 ttcttccgac gcagaggcaa tttctcccat aatgtcgtcc acgcgggtca ccgaactgac
    11341 aatatcgatc atggtcgcgg cggcgttatt caccagtttc gaaccctgct ggacacgatt
    11401 gactgactct tcgatcagcc ctttgatctc ttttgctgcc tgcgcgctac ggctggcaag
    11461 attgcgcact tcacctgcca ctaccgcaaa accacgcccc tgctctccgg cgcgagccgc
    11521 ttccactgcc gcattcaggg ccagaatatt ggtctggaaa gcaattccgt cgataacgct
    11581 gataatgtcg ccaatttttt gcgagctggt ggcgatctcc tgcatggtgt gagtcatggt
    11641 actgacctga acaccgcccg cctgcgccgt tgtcgcggca ttttttgcca gttccgacgc
    11701 ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt aattgctcca tactggcggc
    11761 cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt gagagatcgt tattgcctgc
    11821 gacaatctcc gcgataccaa tgtgcatctc ctggcttccc ttacgcacat cactcaccgt
    11881 cccacgcaaa gcctgctgca tggtcttcag gctggcaaaa atggcggtga tctcattacg
    11941 accatatacc gcaatcggac gcgccagatt acccgcagca atgctgtcaa aatggctacc
    12001 gataatggcc agtggttgaa caatcatctt gcgcgtccac cacagcgcac tgctgatgta
    12061 gatcgccgca acaataatca tgctgataaa caccagcgcc gaaatctgat agttacgctg
    12121 gctttgcgca ctggcggctt ccagcacatg gttgatctcc agctgccagg cctcaaagtt
    12181 aacgtcaaac gcattctgtg attcctgcac cggcgcagtg aggaaatccg aaagttgatt
    12241 actttcaagc caggtcgcct gatgttcgag atcgttgttg tgccagcggg caaaactttt
    12301 ctccgtctct ttttgcaacg ccctgacgtg ctcgttgccc gcagtcatcg ccataaaact
    12361 tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca acgttttaat
    12421 gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct ttgttcagcg cggtactggc
    12481 ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc tctgctcgac
    12541 ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt tactgccaat
    12601 ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt
    12661 aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc gtcaaaatgt
    12721 ttcccagttt ggagcttgtt cagcaattcg cagtcgtggc tgtgccggtg gttgctcact
    12781 ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacggaacgc
    12841 ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc gcagcggcgg cggcagctga
    12901 ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa ccgccaatgc
    12961 gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc ccataatgtc
    13021 agtcacgcga gtgacagcat tgacgatatt gttcattgtt tccccggcgc tttcgaccag
    13081 caccgaaccg gtatcaacgc gtgagacgga gtcttcaatg agggctttga tctcttttgc
    13141 cgcctgggcg ctgcgactgg caagattacg cacttcaccc gccaccacgg caaaaccacg
    13201 gccctgttca cccgcacgcg cggcttcaac cgcggcattc agcgcgagga tattagtctg
    13261 gaaggcaata ccgtcgataa cgctgataat gtcggcaatt ttcttcgaac tatcggcgat
    13321 ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga
    13381 ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc
    13441 ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttcagtacg
    13501 ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga tggcatctga
    13561 accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg
    13621 cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca agttaccacc
    13681 ggcgatttcg cgaatgtgag caataatttt tgccagcgga gtaagcaaca tacggcgaat
    13741 gccgtaccac gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca
    13801 ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact
    13861 gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg
    13921 agcgaaataa gctccagtat tgccataatc aagataatca atcagttcag ttaacgctgt
    13981 gtgatagttt ttatattttt catcaatatt acgactggta gcgaccattt caggtaacgg
    14041 tgccatgctt ttgaattttt tataatgagt cgcggcctgg gccaatgttt tcctggcgct
    14101 atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca tcatccgcac
    14161 cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg acgtcagctc
    14221 gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat ggtgaaggga
    14281 agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca gcaccattac
    14341 caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa acaagttaat
    14401 atcgttatcg gcaagaaggg gggaaacttt attgctgatg ccacccgccg cgaaattgaa
    14461 ataaaaaacc cgatgcgcag atcatcgggt tcatttcaat tgaggaaatc gggagaatta
    14521 cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca gcagtttttc
    14581 gatgttcacc agaatcaaca tccggtcgcc cagtgcgccc agtccagtga gatattctgt
    14641 tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc tccgccgtca atgaaagcac
    14701 gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca ggacgataac
    14761 taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta agtcaacaat
    14821 cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg tgttcgcaat
    14881 ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa taccgtactc
    14941 ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg ccagctttgt
    15001 tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc ggtgttcgcc
    15061 atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag tgccacgctg
    15121 ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt actttcaagg
    15181 tttttaacca caacctggtg ctgaccaatt aattgatcca ccagcaaggc gtagcggcga
    15241 ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc gcccgcgacg
    15301 ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc cagcacccgc
    15361 tcgccgccgg ccagtggatg cagatcggct tcacggggtt gcagtgattc cataacagca
    15421 ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc gaggatggcc
    15481 agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga ctggatttcg
    15541 acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac gccgcgcccg
    15601 gagacgtcgg tgacctgctc tgccgtggag aagcctggtg caaatatcag catcgcgact
    15661 tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt tgccagaatt
    15721 cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt gccgccctga
    15781 tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag ccgtttttct
    15841 ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg gtcgataatg
    15901 cgttctatca ggcttttgtc gagttcagta gaactgccca ccagcgtcag ttctacctgc
    15961 ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa aacatattcc
    16021 atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt acgttgtaac
    16081 tgccccatgc tggttatcaa atcaccatga ttaaccgggt ccagttcgct ggaacgctgg
    16141 gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg atcaaccttt
    16201 tctaccgcta cacggatgct ggtggattca ttgctgcgcg tcgttttttc ccgctccacg
    16261 cggccggttg gcgcttgttc ggctgccagt ttaagcactg gtggggtgga tatttttggc
    16321 gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca gagtaccgct
    16381 gtgatgtcat cttcggcgat gtcgcccggt aatattgccg agagcgaatc cgcccctttc
    16441 accacgtcag ttaacgttgt cagatgtccc agttcttctt ccagcaggtc gacttccccg
    16501 gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac tgcgactctg ctcatcttgc
    16561 ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc gcctttcgct
    16621 tctaatgcca gttgacgcaa ggcctggcag atataatcga agctggcggc atccggctct
    16681 tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc caaaaacaga
    16741 ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag caggttttcc
    16801 atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc tcctttgatc
    16861 gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc cggctgcaaa
    16921 accagcagat gctgctccat gtcagccaac agttcgtccg cttcatcaaa aaatgtctga
    16981 taaaaatcgc ttatatccat gctcacgctg tcacctcggt tcggctgatg gcattgtggg
    17041 aacactgacc tgtggtgcaa cctcaggttt ttccagggcg cttactggct cattctggct
    17101 ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca gcaggctgat
    17161 gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg ccgccatgcc
    17221 gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc ggcgggatgc
    17281 attggcccga tcggcagaaa gctcccagtt gctatatcct ttctcaccgc tggcgtaggg
    17341 gaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca gtacaggcgc
    17401 aatggcacgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg ttctaaacat
    17461 cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca gatcgatttt
    17521 gagatgggga cgtaacgccc gcagtttcgg atcggactct atcaactggt cgagatcacc
    17581 ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga tgttcggctg
    17641 cttattcact tccccctggc tttgggtgta atcatcacca ccgccgggaa ttgggctttc
    17701 actattagaa atgagatcgc cgcccgtaac cgcagtcgcc agtggagtcc ggaagtactc
    17761 cgcaatctga atcagctctt ttgggctgga gatggagatc agccacatca ccagaaaaaa
    17821 ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat gtgctgcccc
    17881 gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat tcttcatgct
    17941 tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc ttccagttca
    18001 ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac ggcgataggc
    18061 ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat cattttgctg
    18121 gtttcggcgc ttttctgacg taaaacactc gctaatgggg aaataaatcc gtaagccaat
    18181 aaaatgccga ggaaagtccc caccatcgca tgtgcgataa gcgcacccag ctcggcggca
    18241 ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta cagccgcgac aataccaaac
    18301 gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc ctcgctttcg
    18361 tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat atgaccgctg
    18421 ataatcagac gcagataatc gacgataaaa tcaagcatga cgctatccgc gaggatgcgt
    18481 gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc cagcgaaaac
    18541 atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag atccatatac
    18601 attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgccttcag cgtgccttta
    18661 atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc aataatcacc
    18721 agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata accgccgaaa
    18781 actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt
    18841 tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggca aaaaaaagca
    18901 gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta aacagcctgt
    18961 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
    19021 ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
    19081 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
    19141 ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagtgg tccttcttct
    19201 gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
    19261 cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt
    19321 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
    19381 ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
    19441 agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat atcccacgct
    19501 tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag
    19561 gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga
    19621 gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga
    19681 agtgacaaac cagttgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg
    19741 ctaacgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct
    19801 gaacaatcaa acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca
    19861 gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt tatgcggttt
    19921 caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca ttagaatagt
    19981 tgcgataagc tgcaataagc agaaccacct ttttggttta atatgtcctt acaaatagaa
    20041 atgggtcttt acacttatct aagatttttc ctaaatcgac gcaactgtac tcgtcactac
    20101 acgcacatac aacggagggg ggctgcgatt ttcaataatg tgtgatgcag atcacacaaa
    20161 acactcaatt acttaacata aatgtataag tcatactttt gttttgggtg tatttcaatc
    20221 tgttaaaaag tttttcgcta cgctagcaag caaaaatgaa acaggaataa tcgaaatggg
    20281 atgttgcgca cagttaaaat aactcaccgt aaataatcat ctgctataaa taatcacttt
    20341 catgcaatac cagataagct atttttaaac agacacttac cgcacaacaa actaattaaa
    20401 tacaaagaaa ggcaacaatc aacttaatgt taatgaaatg ttttacatat attaaccact
    20461 tgtttaatgc ggagaatcag cacactattt tcttataatt acatttgaaa ttatatgatc
    20521 accgggagtg attgattaat tatctttact aataatcaga ctaatattta cctgcttgac
    20581 cgagttgaga ttgcgtcgtt tctccattag gagtaaagct ttaatgtcac ctgaagttca
    20641 cagaataagg aacaggttat gagctatagc aatattcttg tcgctgttgc ggttacaccg
    20701 gaaagtcagc aactgctggc aaaagcggta tctatcgcca ggccagtaaa gggacacatc
    20761 agtttaatta ctctcgcttc cgacccggaa atgtacaatc aattagccgc gccgatgctt
    20821 gaagatttac gcagcgtgat gcaagaagaa acgcaaagct ttcttgataa gttaattcag
    20881 gatgcggggt atcccgttga caagacgttt attgcctacg gtgagttaag cgaacatatt
    20941 ctggaagtat gtcacaaaca tcatttcgat ttggtgattt gcggtaatca caatcacagt
    21001 ttcttttcgc gagcatcctg ctcggcgaaa agagttattg cctcaagtga ggtcgatgtg
    21061 ctgttagttc cacttacggg agattaaccg ctcctacgca agctttggaa aggtagcaac
    21121 tttatcgcgc tgctggcttt ccgcgcttcg cggaactatc tgctttaggt cgctaatgaa
    21181 gcactcctgc cagtggttaa tatcgttttt cacgataacg tccagcattt ctgcatgacg
    21241 ggaaatacgt tccgccagcg acatagtcaa tgcacgatcc agcgcagctg caacttcgtc
    21301 acgatcgtag gggttaacaa ttaacgccga cgttaactcg tttgccgctc ccgcaaattg
    21361 cgaaagaaca agaacgcccg ggttggctgg gtcctgagca gcaacatact cttttgctac
    21421 caggttcatc ccgtcacgca gtggcgtcac taagcccacg tcagagtagc ggaatatttt
    21481 catcagtaat ttacggtcaa aatgctgatt caaataataa agcggcgtcc agcctaattg
    21541 cccgtattta ccattaattc gtccagcttc attttcgagc tgatgacgaa tatcctgata
    21601 ggcttgcaca tcaccacgcg acgttggtgc aatctgggta taacgaattt taccatgatg
    21661 ctgcggatat ttttccagca acgcttcata ggcgagaaaa cgctctggca aacctttgga
    21721 ataatccagc cgttcgacag aaaagatatt ttgtacgttt ttcagttccg ctttaagttg
    21781 cgccagtttt ggcggcagtg gcccggcagc ctgtttggct atttctttcg gttcaatgcc
    21841 gatcgggtag acttctgttc gaaatgcttt gccccaggct gtatggcttt ttgcgctacg
    21901 tgtcgtgacg cgggtcaggt tagaaagaca atccaggaac gccagacgat cgttttctgt
    21961 ctggaaaccc agcaaatcat aatcacaaag ctgttcaagc aaggtgtcat atgtcggcag
    22021 cgcgttgaag atttccggtg tcgggaaagg aatatgcaga aagaaaccaa tgcgattatt
    22081 cactccccgt ttgcgtaatt catgcgcaaa tggcaacagg tgataatcgt ggatccagat
    22141 aatgtcatcg tcttgcaaca gcggcagtaa tttatctgcc agcaacgcat ttacgcgtag
    22201 atagccgtcc caggcaggac gctgaaattg caccagatcg agccgataat gaaaagcggg
    22261 ccagagaacg gcattggaga attggttgta gtattcgtca aggtcctgtt cgctgaggtt
    22321 aaaagaggcc cacgtaatgt tacctttttt cacctttttt agcggctgat cctcattccc
    22381 tgtttcacca ctccagccaa accacagtcc gcctgcggct ttcagtgccc ccagtatgcc
    22441 aacggcaagg ccaccggcac tggcggcgtg ctcgtctggt ggtgcaatcc ggttagatac
    22501 tacgactaaa cgactcatag tcatcactcc tgttattttc tcttttttgt tgtaatgcgg
    22561 tggttatcat ttcaagccag ctccagacat ccggcacacc cgccagtcgc catgatgcct
    22621 gagttgcacc tgtgccaatt tttactgaca ttccgcccag tcggttaacg actgcgaagc
    22681 cagattcatc ggttaaatca tcgcccagaa atacgggcgt tcgcccgata aagggagctt
    22741 cctgcataaa agctgcaatt gcctcacctt tactggtacc tctcggtttg atctcgacaa
    22801 cacactttcc ctgctgtaac gccatttgtg gccagatctg agtaatacgt tgcgctaatg
    22861 tcattaatgc gtcttcatgc tgcggagcct gacgataatg cagcgcaaaa gccatccctt
    22921 tcgcctccag atccgcgccg ggatactgag cgatgactgt atgcagttgc acgctaatat
    22981 cacgcgcaat cgcatccggc agatgaacga tatgtgtttt accattgatg tcacggcgct
    23041 ccgccccatg cacgcctgct aacgggaagc gataaggttt tgccagtgcg tcaagctcca
    23101 ccattgagcg ccctgatatc aatgccaatg caccatcact tgcggttgcc agtagctgta
    23161 gtccttgcag aatattgtca ggcacgacga cctgatcggg atgcggtttg atttccgcca
    23221 gcgttccatc aagatcaaaa aaccaggcat atttcgcgga tagttcaggg gtttcggtta
    23281 acggttctgt cacccggctc tcctccttct cttttcttat tgttttgcgc agaacgcgct
    23341 ttacagacat gtaagtatag acagtgtgac gggggtcgcc atttgcaatg aagaacagcc
    23401 aaccgttgtg gcggctggct gtggtgggaa aaaacgttaa attgttgtgg aaaaaagcat
    23461 cagacagtgc gtttcgcttt ttgcttgtaa cggtcgaaga tcaccgctgc cagcaggatt
    23521 aagccgcgaa ccacgtactg cgcgaaagga gaaatattaa gcaggttcat ggcgttttcc
    23581 acggtgccta aaattaagat acccgccacc acatatgaga tttttccgat gccacctttc
    23641 agagaaacgc cacctaaaac gcaggcggag ataacaataa gctcataacc aatcgacgtc
    23701 attggctgcc cactagtcat acgtgaagcc agaataattc cggctatcgc agataccagg
    23761 ccagagagaa caaagataat aattttggtg cgaacaaccg gtacacccgc cagacgcgca
    23821 gcctcttcgt tcccgccaat cgccagggtg ttacgaccaa aggtggtttt attcagcaac
    23881 aaaccaaaga taatcagaca cgcgacggtg agccagattg gcgcaggcag accgaaccag
    23941 ttagcgtaac caagggcaaa gaagctttca tcttcgatac cgaccgcttt accgtctgaa
    24001 atgatgtacg ccagacctcg aacaatctgc atcgttgcca gtgttgtgat cagagcattt
    24061 attttcagtt tggcgataac aaagccattg accaggccac agagaatgcc aagcagcaac
    24121 cccgctgcca cgccaatcca caggctttca gtcaggttga taaccaccgc cgtggtgaca
    24181 cccgcacagg caattacgga ggcgacagaa aggtcaaagt caccggaagc aaggcagaac
    24241 aacatgccac acgccaccat ccccgacatg gaaattgcca ggcccaaccc tttcatatta
    24301 atgaaggtgg caaaatttgg gacaaaaatg gcacaggcga taaagagcac cgcaaacacc
    24361 accagcatgc cgtactgatc ccagatacgc ccgaagctga atgacgactt aggtgcgcca
    24421 gaccccgatg tagaaacaga agacatcata ctctccttac tcaggcaact gcctggctga
    24481 ctttaggcat cgcaaggctc agtgcctgac gctcatctgc ctgctcgtgt aacaattcac
    24541 cggcgatttc accttcccgc atcaccacaa tccggtcggc aacgccgagg acttcaggta
    24601 agtcgctgga ggcaaacagc accgccacac cctgcgccgc cagcgcataa atcacgttgt
    24661 aaatttcatg cttagcacca acatcaatgc cgcgcgtagg ttcatccagc aaaatgacct
    24721 tcatctcttc cgataaccag cggcccagaa tggctttttg ctgatttccg cctgagagat
    24781 tcatgatcag ttgctcagcg cccggcgttt tgatgttgag cgaacgaatg tggtgatcgg
    24841 cattgttttc ttcccaaccg ttgttgatta cacaaccgcc gagcacatgt ttacgtctgg
    24901 cactgatgtt gatattgtcg cgaacggagt gcacgggaat aatgccttcc gctttgcgat
    24961 cttccgggca gagcatcatg cctgcggcaa tggcgtggct cggtttacga atatcgatcg
    25021 gctgttggtc gatataaacc tgaccggcgg tgatttgcgt cccgccaaac aagcctttca
    25081 ttaattcgct acgccccgcc cctaccagac caaacagccc aacaatttca ccactgcgaa
    25141 ccgccagact tattggcgta cgcacgcctg gtgctttcac agcatcaaga cgcaggcgct
    25201 cctcgccata actacgaggt tgccagccgt agatatcgcc aatgtcgcgc ccgaccatcg
    25261 cctgcaccag cgcgtcgtgg tcaacctgct gcatatcggt aaaggttttg acataacgtc
    25321 catctttaaa gacagtgatg gcatcgctga gggcaaatat ttcttccata cggtgagaaa
    25381 cgtataagat cacccgcccc tcttttcgca gttcacgaat aacgcggaaa agattgtcga
    25441 tttcgcgggc agaaagggag ctggttggct catcaaaggc gataatttta gcgttacgcg
    25501 ccagcgcctt ggcgatttca accatctgcc actgaccaat ggagagatat ttcagcggcg
    25561 tgtccgggtc aatatccata ccaagatgtt taagttgtaa acccgcctca taattcagca
    25621 atgagcgatt cacaatgccg cctttatgcg gcagctggcc gagatagatg ttttccgcga
    25681 cggtcatttc cggcacgaga tgtagttcct ggtaaataat cgccacaccc gcgttaagtg
    25741 ctgcggtcgt gtcggaaaag gacatttcct gcccattaat cactacagaa cccgtggttg
    25801 gcgcatagtt gccgctgagg atttttaaga gagttgattt tcctgcgcca ttttcaccca
    25861 tcaacgcatg aacctgaccg gcatagcagt caaaactaat atccgtcagc gccttaacgc
    25921 cgggaaacgt tttaccgatg ccgcgaaatg agagatacgg ggtagactgt tgcataacgt
    25981 ctccgtgaat cactggtcgt tcacaccccc tctgcatcat gcagagggga atttttccgg
    26041 caaattactt accgcctaaa ccttttttct ccagttcttc tttaaagttg tcacgcgtga
    26101 tcagtaccac gtcggtaact tcggtaaatt ttggcggttc aacgtctttt gctacccagt
    26161 tgtaaagcat ttcgctggat ttatagccat gtacgtccgg gcttggcagc agggaaccgt
    26221 agaagccggt tgcctgtgct ttagacagtt cgctcaccgc atccacaccg ttaatgccaa
    26281 tgccgatgat atcggccgct ttaaagccct gaccttccgt cgcgcgtacg ccgcccagca
    26341 cggtgctgtc gttcataccg acgatcagcc aatgtttaac ttccggatgt tgaaccagca
    26401 ttgagttggc agcgtcaaat gcccccggga tgtcgttaga tttggtaggt acctgataaa
    26461 tttgtttttc cgggaatccg gccgctttca gcgcatccat agatcccgta gtacggcggc
    26521 gggcggtatc cagttcgttg gcggtaatcg ccatcaccgc gctttctttg acatcccagc
    26581 cacgtttctg catctcttta tacagttcct ggccctgacg ttcgccaatt ttagtcgccg
    26641 ccatcatcac cagcggaacg gtatccattg gcttaccttt ggcgttaaca aactggtcat
    26701 ccacggcaat gactttcata tcgtagccac gcgctttcgc gacgatggca gagccgagtt
    26761 tggggtccgg agtacaaata acgaaacctt ttgcgccact ggcagccagg ctgtcgatcg
    26821 cgttcaatgt tttttcgcca tccggcacgg caatcttaat aacctcaaac cctaaatcct
    26881 tcccggcttt atcggcaaac ttccattcgg tctggaacca cggctcttcc ggttgcttca
    26941 ccagaaaacc gagcttcagg ttctccgcca tagcggattg tgacataacg gctgccagac
    27001 caatggctgc cagggcttta gtaaatttgt gcatggttct ctccagcttt agtgtcgttt
    27061 tgtgtagggc aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag cattaccttt
    27121 taactaaaag ataagtgact gtgttgacat agttttagcg agaaattaat tctccatagg
    27181 agagcaatat cacatcgcag aattacagtg agaacgtgca taaatttagc gggaaaagac
    27241 ataagggaaa gccaatttgt cagacaaatt gtcgaatgca cagcagatta atccataaga
    27301 ttagcctgga aatccttgtt gtctttggta cccatgcggg atgtcttctt tttaaccagt
    27361 caataggccg cattacctgg cgttgagttt ttgaaatggt gtaataaccg caactcaaag
    27421 atgtggaaaa tgcacgtcat tcatttcgcc attaattatc actgtactca ttaattaaca
    27481 gaacacgtat aacgagagcc atctcgcaaa aatgaaaaaa cgttttataa aatcatcact
    27541 tcatcatgaa ttcaaattca ttgattaata tcaacaagat acaaaaagcg ctatcattaa
    27601 aattcattgc agttacattg atttcatcaa tgaaatgtaa aaatatataa acttgatgat
    27661 ttaagcattt tcttataccc gttcagacgt tattcttatt tcagatcatc gtcagaattg
    27721 actccacgat cacatttcgg accggcagaa aggaattatt ctgcaaacag taattatggt
    27781 gttttgattt atcttgcacc tctccacttc tggatataag gatattaggt atggcaaccg
    27841 ctggaatgct tctcaaactc aactctcaaa tgaaccgcga gttttacgca tccaatctct
    27901 accttcacct gagtaactgg tgttctgaac agagtctgaa cggcaccgcc actttccttc
    27961 gcgcccaggc acagagtaat gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg
    28021 tcggcgctac ccccatcgtt aaagccattg atgttcccgg tgaaaaactg aactctctgg
    28081 aagaactgtt ccaaaaatcg atggaagaat acgagcaacg ttctagtacg ttggcacagt
    28141 tagccgatga agcgaaagaa ctgaatgatg attcaaccgt caatttcctg cgcgatctgg
    28201 aaaaagaaca gcagcatgat ggtctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg
    28261 cgaaacttgc gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac
    28321 accagctgca ttgatcatca tcggcgctaa tgcattgcgc cgatgaaggt tttgagaaac
    28381 cgctgcctca tctgtttgaa gcagcggttt ttttaatggg attcaccctg tggggtaaac
    28441 ttgagttcaa taagcgcgat ggctttttgg attgcccgca tggtgacagg gtctgcggcg
    28501 gcgggatggt tagtaaagtc gatattcttc agctgactgg acattttttc acgaacttca
    28561 acgggcgcga ttacatcgag aacatccaga atttgtttga taaccaactg gcaagcaacc
    28621 acatcagaaa ccagttcctg atcggcattc agcggctggg acatcgtaaa ctcctgatag
    28681 cattttgaaa gccgttatag tagcggcttc acatcttcag cgatagtcac atccaccgtc
    28741 atcaggacac aaaaaaacct gccggagcag gttttttatt atcggaacat attgcctggc
    28801 ggtacgtctt tgaacgtctt gcaatagtta ttgaacatac ttttcaggat tttgcgcagt
    28861 ttcatcgcgg cactccgacc atttgttata caggtgttat tctctttgcg cttataatat
    28921 gacaaccatc acaaaaatca atctttatgt gatacaaatc acatagatac ccctttaatg
    28981 ttataaaaat gataatcaaa aaacagcccc cctatttctg acacctacag atggcaagaa
    29041 atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc gtaaaatagt
    29101 gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct ttcattaata
    29161 acgctggcgg ggtgtacggt tactcgccag gcccatgtga gcgaagttga cgcggcaaca
    29221 ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac cgatcgttat
    29281 gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca cgcggtcccc
    29341 tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt gaatacggaa
    29401 ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta aatattttaa
    29461 tcagcgaggg gatcttcgct gattaaagaa atagcggtaa tgcgttttat tcccattcgt
    29521 atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa caaattatag
    29581 tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga
    29641 ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa
    29701 tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg
    29761 cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt
    29821 taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat
    29881 tccaggaaac ctataaacac gaacaattaa tcacgcagaa aattaacgaa ctggctcatg
    29941 ctgcaatgac caatcaggac tacccaacat ttaatttcct gcaatggtat gtttctgagc
    30001 agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa
    30061 gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat
    30121 gctctcaggc ggcaggtttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg
    30181 atcggtcgaa aaaccttctt ctgaaggtat aaatttgcct tttgctgcca gaaacgctac
    30241 cagctctccc gcagtcatcc cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa
    30301 acgcgcttta attgctgctt ccagactggc atgcgtatat tgctcgcctg attcaatcat
    30361 catatttaac acttcatgac cgtgaataga gtccatcgtc cctcctcaaa aaaagcctag
    30421 cgtagcgatt gccgcttatg aagactttgc gccagcgcag gactgaatgc tttttattgt
    30481 acatttatat ttacaccata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc
    30541 tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac
    30601 cctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc
    30661 gctggctgcg gccggtgttg gttttagcgt tacgttaatc ttgttgattg ggctttgggc
    30721 gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac
    30781 cggtcttggc acgctggcaa aacgctatct gggacgctac ggtcaatggc tgacgggctt
    30841 cagtatgatg ttcttaatgt atgctctgac tgcggcatac atcagcggtg ccggtgaatt
    30901 gttggcctcc agcatcagcg actggacagg tatttcaatg tcggcaaccg ctggcgtgct
    30961 gttgttcact tttgttgccg gtggcgtggt ttgtgttgga acatcgctgg tcgatttatt
    31021 taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg tattgctgct
    31081 accgcatatt cacaaagtga atcttttaac cctgccgttg caacaggggc tggctctgtc
    31141 agcaatcccg gtgattttta cctcatttgg ttttcacggt agcgtgccga gtattgtcag
    31201 ctatatggat ggcaacgttc gtaagctacg ctgggtgttt ataaccggta gtgcgatccc
    31261 cctggtggca tatattttct ggcaggtggc aacgcttggc agcattgatt caacaacctt
    31321 tatgggattg ctggctaatc atgctggatt aaacgggctg ttacaggcgt tacgcgaaat
    31381 ggtggcctct ccgcatgttg agctggcagt gcatttattt gctgatttag ccctcgccac
    31441 gtcatttctc ggcgttgcgt taggcttatt tgattatctg gctgatttat ttcagcgttc
    31501 aaataccgtt ggtggacggt tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt
    31561 tgcactgttt tatccacgag gatttgtgat tgcgctgggt tacgccggtg tggcgctggc
    31621 ggtactggca ttgattatcc cttcgctgtt gacctggcaa agcagaaagc acaatcctca
    31681 ggcgggttac cgggtgaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat
    31741 tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc
    31801 agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa tttgacctct
    31861 taatgcaggc agcactgctt aaatttctta ccactaccgc acgggcaagg atcgttacgc
    31921 cccggtttct cttctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc
    31981 cagtacgcat gtagatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct
    32041 tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc
    32101 tccagcgctg gttttaacga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc
    32161 atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag
    32221 ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata
    32281 tgttgaaaag ccagattcat aaagcgcgtc atctctttct ctgacgccca gcgcggcaca
    32341 tagtcagccc caccccacac ggcaaccagc cactgttccg gttcaatctc ttgcggagaa
    32401 ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc gtggtcagtg
    32461 ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt
    32521 ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt
    32581 atcagagata ctttttgagt ggctttgctg gtgattaaaa attagggagg gtgtaacgac
    32641 aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attgctttga
    32701 ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg
    32761 ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt
    32821 ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct
    32881 ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgac gccgatgtct
    32941 tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
    33001 tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta
    33061 tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag
    33121 tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttacgccgaa acgatcactg
    33181 atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac
    33241 cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag
    33301 cagccaggcc aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc
    33361 cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc
    33421 acgggcgatc atcgttgccg ccggtagggt cacccaccag ctgtgataat gctcggttac
    33481 cagcaccatg gcgatagcca cgagaacttt atctgctaca gggtcaagga aagcaccaaa
    33541 ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc
    33601 gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa
    33661 tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg
    33721 cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat
    33781 caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt
    33841 gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac
    33901 atttgccgac gttttggccc gacgccttca atggtttcca gggagctggt atttttgacc
    33961 ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcgt gtgattcatc gcgaatatgc
    34021 tggataacat gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca
    34081 aagaacagtg tttccagccc aaccttacga tctactcctt tggcaacgcc aagtagcagt
    34141 ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc
    34201 tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta
    34261 ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct
    34321 gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag
    34381 gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact
    34441 tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc
    34501 gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc
    34561 ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa
    34621 tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg
    34681 cgcatctggc tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt
    34741 tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga
    34801 atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcgagg
    34861 tcgtcgccgg tattggaaac gaattgtttt tcggtgacgc gtcgcaccgc ctgaatttgg
    34921 tcgcgaatac gtgcagcttc ttcaaactcc agattctggc tggcagtttc catacgacta
    34981 atgagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc
    35041 tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct
    35101 atctggtatt gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga
    35161 atggggaaaa tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac
    35221 gggccgaaat attcaccttt ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg
    35281 gtatcaccac tcaggaagat aaaaggatat gatttatcat cgcgtagcaa aacgttgtaa
    35341 cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtgtga
    35401 gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg
    35461 aggttgctac ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag
    35521 ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt
    35581 aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca
    35641 gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga
    35701 acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct
    35761 cattgacctt ctggcccttg gtgatcatca gcataatctg caattcacgt tcagacaaac
    35821 tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga
    35881 tgtcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct
    35941 gcggagccgc gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta
    36001 aagggttttc tgtatggacg gtaagcatga tgattttgac atcagctgtg gaacgcgcga
    36061 ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt
    36121 caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatacc tcaccgacga
    36181 cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt
    36241 catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga
    36301 tagttagtca ttcgtatatt agctgttttt attgcaactt tgaaacgtta aaaatggtgg
    36361 gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt
    36421 caccaacggc ttaccggatc gtttcacagt aagccctacg atgaaatgtg acaaaaattg
    36481 actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac
    36541 aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt aacgtatttt
    36601 atagcactgc cgatatcacg ctcaaaaaac aaccactgct atttttagca aagcttatgg
    36661 tatactccgc cgccttaaca tttttcaccg caaattttca ttgcaacatg acgaggaaaa
    36721 taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc
    36781 tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc
    36841 cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca
    36901 gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc
    36961 tgagagcgtg cctttcagcc ggtttttgca tctggcacgc agaaagaaaa gcgatcaaat
    37021 taagccagta gcggccgcgt aacaggccac ctgggtctta tttggtgcat tgattttttt
    37081 ctgcatgttt ttctgatgga aattgaccgt attctcagag attgacaaaa tcatcgctat
    37141 ctctgctgat gtcttccctt ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa
    37201 attcatctct ggcgtcatca ctatttcatc atttaaacgc atcagagcca tcagactttc
    37261 gcgcaccagt aactgcattt ttaattgcag ttcatcacta agaatgggta tttcgcgcgt
    37321 gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt ggcagcatta aatactgagt
    37381 gacaccgcgg cgtaaaccat gtgcgcgcgc ggcttcccat aacggctgtg cttcgctgaa
    37441 taagtcatca ttccacatta aatggccctg actaaagttt tcagggttca gcaccggatc
    37501 aattgcgaga aagttttttg cctgataata actaacccac gcctcagggt aattggtgta
    37561 gaaagccact ttaggtcgcg tgaatggtac cgggtggcga acacataacg aatagtaatc
    37621 gtactccagc tgctgagcct gaagctctat ttcatggtag acctcttctg cggcctccat
    37681 cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt
    37741 aaaccgcaac gcccctgaga gtgagtatca tttataatga taattca
//
LOCUS       DAZGTR010000032        37742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGTR010000032 DAZGTR010000000
VERSION     DAZGTR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.032"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            134..271
                     /locus_tag="ACHLCY_001831"
                     /pseudo
     CDS             134..271
                     /locus_tag="ACHLCY_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(379..1614)
                     /gene="aslB"
                     /locus_tag="ACHLCY_001832"
     CDS             complement(379..1614)
                     /gene="aslB"
                     /locus_tag="ACHLCY_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0369104.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMHAHLLVVSK"
     gene            complement(1761..1837)
                     /locus_tag="ACHLCY_001833"
     tRNA            complement(1761..1837)
                     /locus_tag="ACHLCY_001833"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1801..1803),aa:Pro,seq:tgg)
     gene            complement(1880..1966)
                     /locus_tag="ACHLCY_001834"
     tRNA            complement(1880..1966)
                     /locus_tag="ACHLCY_001834"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Leu,seq:cag)
     gene            complement(1987..2062)
                     /locus_tag="ACHLCY_001835"
     tRNA            complement(1987..2062)
                     /locus_tag="ACHLCY_001835"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2027..2029),aa:His,seq:gtg)
     gene            complement(2121..2197)
                     /locus_tag="ACHLCY_001836"
     tRNA            complement(2121..2197)
                     /locus_tag="ACHLCY_001836"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2161..2163),aa:Arg,seq:ccg)
     gene            complement(2300..3685)
                     /gene="yifK"
                     /locus_tag="ACHLCY_001837"
     CDS             complement(2300..3685)
                     /gene="yifK"
                     /locus_tag="ACHLCY_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0369105.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3876..4616)
                     /gene="wecG"
                     /locus_tag="ACHLCY_001838"
     CDS             complement(3876..4616)
                     /gene="wecG"
                     /locus_tag="ACHLCY_001838"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP0369106.1"
                     /translation="MNNNTMAPTYTLRGLQLIGWRDMQHALDYLFADGQFKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4619..5971)
                     /gene="wzyE"
                     /locus_tag="ACHLCY_001839"
     CDS             complement(4619..5971)
                     /gene="wzyE"
                     /locus_tag="ACHLCY_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP0369107.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5968..7047)
                     /gene="rffT"
                     /locus_tag="ACHLCY_001840"
                     /gene_synonym="wecF"
     CDS             complement(5968..7047)
                     /gene="rffT"
                     /locus_tag="ACHLCY_001840"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP0369108.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(7044..8294)
                     /gene="wzxE"
                     /locus_tag="ACHLCY_001841"
     CDS             complement(7044..8294)
                     /gene="wzxE"
                     /locus_tag="ACHLCY_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP0369109.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPATIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8296..9426)
                     /gene="rffA"
                     /locus_tag="ACHLCY_001842"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8296..9426)
                     /gene="rffA"
                     /locus_tag="ACHLCY_001842"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP0369110.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9431..10105)
                     /gene="rffC"
                     /locus_tag="ACHLCY_001843"
                     /gene_synonym="wecD"
     CDS             complement(9431..10105)
                     /gene="rffC"
                     /locus_tag="ACHLCY_001843"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP0369111.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10083..10964)
                     /gene="rfbA"
                     /locus_tag="ACHLCY_001844"
     CDS             complement(10083..10964)
                     /gene="rfbA"
                     /locus_tag="ACHLCY_001844"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0369112.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10983..12050)
                     /gene="rffG"
                     /locus_tag="ACHLCY_001845"
     CDS             complement(10983..12050)
                     /gene="rffG"
                     /locus_tag="ACHLCY_001845"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0369113.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12050..13312)
                     /gene="wecC"
                     /locus_tag="ACHLCY_001846"
     CDS             complement(12050..13312)
                     /gene="wecC"
                     /locus_tag="ACHLCY_001846"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP0369114.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13309..14439)
                     /gene="wecB"
                     /locus_tag="ACHLCY_001847"
     CDS             complement(13309..14439)
                     /gene="wecB"
                     /locus_tag="ACHLCY_001847"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP0369115.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14495..15541)
                     /gene="wzzE"
                     /locus_tag="ACHLCY_001848"
     CDS             complement(14495..15541)
                     /gene="wzzE"
                     /locus_tag="ACHLCY_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP0369116.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNEELKGGWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15553..16656)
                     /gene="wecA"
                     /locus_tag="ACHLCY_001849"
     CDS             complement(15553..16656)
                     /gene="wecA"
                     /locus_tag="ACHLCY_001849"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP0369117.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16896..18155)
                     /gene="rho"
                     /locus_tag="ACHLCY_001850"
     CDS             complement(16896..18155)
                     /gene="rho"
                     /locus_tag="ACHLCY_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP0369118.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18240..18341)
                     /gene="rhoL"
                     /locus_tag="ACHLCY_001851"
     CDS             complement(18240..18341)
                     /gene="rhoL"
                     /locus_tag="ACHLCY_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP0369119.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18482..18811)
                     /gene="trxA"
                     /locus_tag="ACHLCY_001852"
     CDS             complement(18482..18811)
                     /gene="trxA"
                     /locus_tag="ACHLCY_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP0369120.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18942..20207
                     /gene="rhlB"
                     /locus_tag="ACHLCY_001853"
     CDS             18942..20207
                     /gene="rhlB"
                     /locus_tag="ACHLCY_001853"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP0369121.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20341..21825
                     /gene="gppA"
                     /locus_tag="ACHLCY_001854"
     CDS             20341..21825
                     /gene="gppA"
                     /locus_tag="ACHLCY_001854"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP0369122.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21872..23893)
                     /gene="rep"
                     /locus_tag="ACHLCY_001855"
     CDS             complement(21872..23893)
                     /gene="rep"
                     /locus_tag="ACHLCY_001855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP0369123.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24110..24559
                     /locus_tag="ACHLCY_001856"
     CDS             24110..24559
                     /locus_tag="ACHLCY_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP0369124.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24758..25039
                     /gene="ppiC"
                     /locus_tag="ACHLCY_001857"
     CDS             24758..25039
                     /gene="ppiC"
                     /locus_tag="ACHLCY_001857"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP0369125.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25126..26601)
                     /gene="ilvC"
                     /locus_tag="ACHLCY_001858"
     CDS             complement(25126..26601)
                     /gene="ilvC"
                     /locus_tag="ACHLCY_001858"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP0369126.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26751..27644
                     /gene="ilvY"
                     /locus_tag="ACHLCY_001859"
     CDS             26751..27644
                     /gene="ilvY"
                     /locus_tag="ACHLCY_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP0369127.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSIVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27696..29240)
                     /gene="ilvA"
                     /locus_tag="ACHLCY_001860"
     CDS             complement(27696..29240)
                     /gene="ilvA"
                     /locus_tag="ACHLCY_001860"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP0369128.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29243..31093)
                     /gene="ilvD"
                     /locus_tag="ACHLCY_001861"
     CDS             complement(29243..31093)
                     /gene="ilvD"
                     /locus_tag="ACHLCY_001861"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP0369129.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQDARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31158..32087)
                     /gene="ilvE"
                     /locus_tag="ACHLCY_001862"
     CDS             complement(31158..32087)
                     /gene="ilvE"
                     /locus_tag="ACHLCY_001862"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP0369130.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32107..32370)
                     /gene="ilvM"
                     /locus_tag="ACHLCY_001863"
     CDS             complement(32107..32370)
                     /gene="ilvM"
                     /locus_tag="ACHLCY_001863"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP0369131.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32367..34013)
                     /gene="ilvG"
                     /locus_tag="ACHLCY_001864"
     CDS             complement(32367..34013)
                     /gene="ilvG"
                     /locus_tag="ACHLCY_001864"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP0369132.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34016..34066)
                     /gene="ilvX"
                     /locus_tag="ACHLCY_001865"
     CDS             complement(34016..34066)
                     /gene="ilvX"
                     /locus_tag="ACHLCY_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP0369133.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34153..34251)
                     /gene="ilvL"
                     /locus_tag="ACHLCY_001866"
     CDS             complement(34153..34251)
                     /gene="ilvL"
                     /locus_tag="ACHLCY_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP0369134.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34604..36124
                     /gene="yifB"
                     /locus_tag="ACHLCY_001867"
     CDS             34604..36124
                     /gene="yifB"
                     /locus_tag="ACHLCY_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP0369135.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36149..36487)
                     /gene="maoP"
                     /locus_tag="ACHLCY_001868"
     CDS             complement(36149..36487)
                     /gene="maoP"
                     /locus_tag="ACHLCY_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP0369136.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36606..37445
                     /gene="hdfR"
                     /locus_tag="ACHLCY_001869"
     CDS             36606..37445
                     /gene="hdfR"
                     /locus_tag="ACHLCY_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP0369137.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37541..37616)
                     /locus_tag="ACHLCY_001870"
     tRNA            complement(37541..37616)
                     /locus_tag="ACHLCY_001870"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37581..37583),aa:Trp,seq:cca)
     gene            complement(37625..37701)
                     /locus_tag="ACHLCY_001871"
     tRNA            complement(37625..37701)
                     /locus_tag="ACHLCY_001871"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37665..37667),aa:Asp,seq:gtc)
BASE COUNT         9237 a        10285 c         9484 g         8736 t
ORIGIN      
        1 tcattttctg gataaataaa taatttattt ttgtcactat tatttatgta atcatcctgt
       61 cagggagagg gatctcaatt atcaatgctt aattacgtca tcattttgat gacatgaaac
      121 cggagcattt tgcatggaat tttcgttttc acccaaactt cttgttgttg ctgtcgccgc
      181 cgctcttcct ctcatggcca gcgcagcaga taccccgtca actgccaccg cacgcaaagg
      241 ctttgccgga tacgatcacc caaaccagta tccacagaac gctgcaattt attgaatttg
      301 cacgatcatg taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc
      361 cacaaaatgg ccgctttttt acttactcac caccagcaaa tgcgcatgca taatgtcact
      421 ggccgggcga ccgtgcgcca gcaaatcagc cattgcttta agatatggcg gtagatggcg
      481 gaaataacgc tgatacccgg cacacaaata attcagcccc ggtttgccgc tggcatcgag
      541 catgaagcgg tgtttcgggc agcctcccca gcacgctttt aacacgttac aactgcgaca
      601 ctgcgccggt aattgcttaa atttatcttc accaaacacc tgctgttgcg gggaatcgat
      661 catttctgca attgtttgct gatgcatatt ccccagccga tattgcggat aaacatagtg
      721 atcgcaggcg taaacgtcgc cgttgtgctc aacaatcacc gagcgcccac aggttggctg
      781 atgatggcaa accgcacccg gcgcaccgac aaaattggca aacgcccatt cgatattcat
      841 cacgaaaatc ttgccgacgt cgcgtttgat ccagtggtcg aatatcgcca ccagaaattc
      901 accaaattca tcggggcgca ccgaccattc cgtcagttcc ccctgaatat caccaggcgc
      961 atgtagtttc agtccatcgc tggcagctgt ttcatcagcc aggcgctcga ccaccggaat
     1021 aaactggatg aattcgactc ccgcatcgca caaaaaatca taaacctgca acggttgctg
     1081 cgcgctggtg cggttgacgc agaccagcac gttatagtcg acatgatgtt tttgcagcag
     1141 cgtcagggca cgcatcacca gcttatgcgt tggtctgcca cctttggtca cgcgatattg
     1201 attgtggatc tcagccggac cgtccagcga taacccaaca agaaaatgat tttctgccag
     1261 aaatgcacac catttatcat cgagcagcac gccgttagtc tggaagctgt tacttatctt
     1321 cctgccagca ccgtatttcg cctgtaactt tacggcacgg cggtaaaaat ccagcccgag
     1381 taacgttggt tcgccgccct gccaggtaaa agccacttcg ttttgcggtt cgctggcagc
     1441 gatatagtga cggacatacg cttccagcgt gtcatcgtcc atatgcgtga ctggcttttc
     1501 gcggtaaagg gattgttttt cgagataaaa acagtagtca cagttcagat tacaatcgga
     1561 accactcggt ttcgccatca catgaaaagc acgcgttgga acctgttgca gcatggtcac
     1621 tccttgcctg aagtcatcct tcaatacgtt aagggcgaaa tcataccgtg gggcaatgag
     1681 ttattacggg cgtttattgc gaagggaggg tgacgaaatg cacagaaaac aaaaaacccc
     1741 gccgaagcgg ggttcaaaat tggtcggcga gagaggattc gaacctccga cccactggtc
     1801 ccaaaccagt tgcgctacca agctgcgcta ctcgccgaaa tactgctttt tgaattttta
     1861 gttcaattct ttaaagtcgt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
     1921 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcattg tcacaacttc
     1981 taataatggg gtggctaatg ggattcgaac ccacgacaac tggaatcaca atccagggct
     2041 ctaccaactg agctatagcc accactacaa atcttgttac gcggtattac taccaccgca
     2101 gctcaagcgc cgggactaaa tggcgcgccc gacaggattc gaacctgaga cctctgcctc
     2161 cggagggcag cgctctatcc agctgagcta cgggcgctta gcgccgttgc gggggtggat
     2221 aatacggact tcccacccct ctgtctagtc cctttttaaa taaattgcgc gtttggttac
     2281 ggtttgtgcg ttttgctgat tattcctcca gtttatgcgc tttcccgtgg cgattaaggc
     2341 caaaaacttt ataaatcgcc gtcaccgcca gcataaagat gatgccaaca aacagcgaca
     2401 tacgcgtatc ttcattaaag tacatgccga tcaaaacgca aatcaggaat gccattgtta
     2461 cgtagttggc ccacgggaac aggattgagc ggaacggatg gctggcaatc gccgctttat
     2521 gtgcacgccg aaaacgcagc tggcttatca atatcacaaa ccatggcacc atccccggaa
     2581 gcacgctggc actgtagacg tagacaaaca cacgctgcgg attgggaatg atgtagttca
     2641 ggcatgagcc aattagcaga atagcaatag atactgccac acccgcaact ggtacgccgt
     2701 gacgggaaac tttcgccatc gccgccggta actgacggtt tttcgccagt gcgtagagca
     2761 tacgtccgca actgtacatg ccgctgttac agccagagag cgcagccgtc agcaccacaa
     2821 agttgataat gcccgccgct gcggtaatac cgattttggc aaaagtcagt acgaacgggc
     2881 tgccgttgct gccaatttca ttccacggga agatggtgac gataacgaaa atcgcgccta
     2941 cgtagaaaat caggatccgc cacagcactt tgcctacggc actgcgcagc gtcacctgcg
     3001 gattcttcgc ttcaccggca gtaatgccaa tcagctccac gccctggtag gacgccacca
     3061 caatacacag cgcggtcagg aaccctttcc agccacccgc aaagaaaccg ccatgctctg
     3121 tgagattgct aaaaccaatc gactgcccgc cattgccaaa gccaaagaaa atcacgccca
     3181 ggccaatgac aatcatcacg ataatcgtgg tgactttgat catcgcgaac cagaactcga
     3241 tttcgccgta caaccgcacc gccgccagat tcgccaacgc caccagcgcc actgcgatca
     3301 atgcgggtat ccactgcgcc atctccggga accagaactg gacataaacg ccaatggcgg
     3361 tgatttctga gatccccacc gccatccaca taaaccagta agaccaggcg gtgagataac
     3421 caaagaacgg gctcatataa cgatgcgcat aaacggcgaa cgaaccggta accggttcga
     3481 ggaacaacat ttcgcccatt gaacgcatga tgaaaaagac gaatagcccg gcgatgatat
     3541 aggccaacaa tacggatggc ccagcccatt tcaaggtact ggcggccccc ataaacaggc
     3601 cgacgccaat ggtgcccccc agggcgatga gttcgatatg tcgagcttcc agcccacgct
     3661 gtagctctgg tttgttatct gccataaatc ctcgtgttgt gtttgcatgc tttccggtgt
     3721 taccggttat cgttatgggt acatcgagtg ttgcgaatgt tttcgtaatt caggagaaat
     3781 ggcaaataaa gcattaaaaa tttgaatgct ttgtgtaata aaaaagcaga caggcgacgg
     3841 agtgaccact ccgtcgcttt acaaagagag gaaaatcaga ggttgccggt gtagtgccag
     3901 cgtaaataac gcagcaaacg aagctgacgc ttaatgcggc tcggctgcga aagcaggcgg
     3961 tagagccact ccagtcccag cgtttgccag attttcggtg cgcgttttac gtgaccggtg
     4021 aaaacatcgt aagtcccgcc aacgcccata tacagcgcat ctggatgcac cagacggcag
     4081 tcgcgcatga agatctcctg ctttggcgat cccatcgcaa cggtgacgat ttgcgctccg
     4141 ctggcatgaa tgcgttcaaa cagcgcctga cgctgctcgg gtttaaaata accatcctga
     4201 ctgccaacga tattcacatt ccactggttg cgcagtttag cttcagtttg cgccagcact
     4261 tcaggtttac cgcccacaag aaataccggc gtcccttctt tgcctgcgcg cgccatcagc
     4321 tcttcccaga gatcagcacc ggcaacgcgg gaaacctgcg cctgcgggta ctttttacgt
     4381 actgaacgta caacgctgat gccatccgca tatttaaatt cggcagcgtt aattagctcc
     4441 ctgacctcag cattatcttc aatggtcagc attttttcgg cattaatggc aaccagcgtt
     4501 ccctgcttaa actgcccgtc agcaaacaga taatcgaggg cgtgctgcat atcacgccaa
     4561 ccaatcaact gtaagccacg cagcgtatag gtcggtgcca tggtgttgtt attcattgtt
     4621 atccttcaac ctgcgtccgg agcgatgatt ttgtacgttt atgaatgagt ccggcgcttt
     4681 caaaaagcca gtacaacagt tttgcgatca tcagacatgc gccgaagacc acgataaaaa
     4741 agactacgcg tgagacaaac gaatccagcc cttcacgtgc cagcacgatc atattgaaga
     4801 tcgccccaaa gcagaaactg tgcaatatcg cagccttata gcgattaggc tcgcggttgc
     4861 ccagctcata cagccagtcg aaccatttga tgatcagacc aaccacgatc gccccgagcg
     4921 ggatgaacaa cgcgccgccc atcaccacca gtgagcctat aagcgtaggc gagatcgcca
     4981 gtccggagtg gttattcagc acttcccagg taaagtagtt ggctgagttc agcaccatac
     5041 tcgggcgacc cggccacagc caggaaggga taaagacata gaaatcgcgg acaattggag
     5101 ccaggccctg gaagtcgatg ttgtcgtagt tctgcaacag caacgccaga ttctcccacg
     5161 gcgagaaggt gtcgcgagtg agatagagaa acgtatagaa cgcttcatcg ccgctcacat
     5221 tcattccata gcgttttagc gccagccaga acatgcccac aatccccagc acgcccgccg
     5281 ccgccagcat ccacaacgaa atccagccgc gaataatgcc aataaacagg aagatagcga
     5341 atgcgatgat gatattggcg cgagtgccgc cgacaatcat ataagtcagc aagccaaagg
     5401 cgaccgtgct gacgaggaaa aacagccacg ctttgctatc ctggcgcaga aagtagacca
     5461 ccagcatcgc cgggatgaaa aagtaaaaga agcgttttaa cgccacgccg gagacttcac
     5521 tggagaagat ctgactgtag gagttaagcc ggaacagcaa aaagccgttg tgcatgaaga
     5581 agatgccgac gcttaccagc gcgatgccca tcaggatcac ccacgtaaga ttggtctcca
     5641 cgcggttcat ggtaaacagc ggacggcgcg gtacatcagc aacgcgtttg cgtaggcggg
     5701 ttttgtaggt gacatagtaa accgcgtaga agcagcccgc agaaagcaac gcctgcaaca
     5761 agatttctgg cggcgcgaca ccaacatcaa agcgaaatac cagcacgctg gtcagcggga
     5821 agccgaagaa aaaggtgagc aaaaacagca atgaaaagaa gacattgaag ttaaagcgca
     5881 cacggcgaaa ctcaaaccag gtcagcgtgg caataaacag cgtgcagagc agccagacca
     5941 caaacaggcc actgaattgc agcagactca tgcgccctcc ccggcggcaa tcgccaacgc
     6001 ccgctgccag ccttgtagat agttagggct aaagaaggca atggtgtttt tatccaccga
     6061 cgccaactgg cgctgcgctt cacgcacaat atcctcgttg agatcgtcgg tagtaaacag
     6121 cactggcaaa tgctgttccg tcatatcctg ccagaacgga ttttcccggt taagcacaca
     6181 aggaatgccc gcctgaatca gtaagcacag cgtaccaatg ccctgctggc gagcaaaaat
     6241 aaagtaacca agatcgcact gacgaagtag cgccagatag gcgtcaaatt ccagtttttc
     6301 gctcaaaatt tgcagatttt cttcgctgaa taactccagc cccgcctgac gaacttcctc
     6361 aatgtacgct tcgttattag gcggatatcc catcggcacc accactttta ccgtatcgcc
     6421 aaattgctga tgaacggcac gtaaagcggc gacatgctca ttgctgaggt cgccggagtt
     6481 ccccaccaga atggtcattt ttccttcacg ttgccgatcg ttcgccatcg tattgagaga
     6541 agggtccatc cgcgtcggga agtacagcag ttctccccgc acctttgggt gcgttttggc
     6601 aaaaaagctc aaatcgccgc gggtggcaaa tacacagccg actcgctttt gcgccaggcg
     6661 acgtagtggg taaaaaagct tatatctcaa gccactggaa agctcataca ggtctgcccc
     6721 ccagatatgc cagaaaaact ggctgggctt aatgccacca ctcagcagag ccagccacag
     6781 tgtgggattg aactgaccgt ggaaaaaaaa tcgctgctga cggttagctt ttgctttcgc
     6841 gatgaccgct tccgccagcg attttttccc agggaaaaat tgcacagaaa gcgccggaca
     6901 gctatcactt aagccgtcgt ccttgccaac aaccataaac tcgcgcgcgt gctcgctcgt
     6961 cgcagccagc gcgtcattga aaaaccgcaa aacggttcgg ttatggtgag ggatatccga
     7021 tcccagtacg tgaatcagta cagtcatgcc cgcctacgcc agagtaaaaa cacgccacaa
     7081 caaagagaaa aatagacgat ataagttgcc atatatgcct gcgccgcgcc cagtgcacca
     7141 tgcgcaggga ttagccagtg ggcaaatacc atcaataaag tgaactggct gatttccgcc
     7201 agaatataaa accgcaatga cgctttggcg atcaccagat aaccaaagac ataagcgccc
     7261 acttttaaca catcacccac taactgccag gcaaagagat cgcgcatagc ggtaaattta
     7321 ttcgacaaca gcagccagat agcaaaatca cgcagcagcc agacggtaaa actcgccgcc
     7381 gccactgccg gtaagacgaa tttcagcgat ttaaccactt cccgggtgat atcgcgcttt
     7441 tccgttagcc gcgacaacgt gggcagcaaa tatacgctga acgatgccgt aataaattgc
     7501 aggtaggcat cggaaatact gctcacccct tgccagatcc ccacttcatc ccagctatac
     7561 tgcgccgcca gcagtttacg catcatgatg taagcaacag gcaaggtcac cgacgtaatc
     7621 aacgccatga gcgtaaattt gctcaactgc cctgccagac cgttatccca gctgggtttc
     7681 agatagctta acgggatgac accacgtttg atcaacatga tagtggcagg aattaccacc
     7741 agcgcgggaa tcagcgccag acccagcaac gccccttcat aaccgcccaa acggtaactg
     7801 acgtagtacg cgagaacgcc aatcaagctg ccgacaatca gcgataacgc attacctgcg
     7861 gcatcgcgaa agcctttcat cagcgccagt aacaggttgc cccaggcgat ccccatttgc
     7921 accagcgcca ctaaacgcac cagcccctga tagtcggtat taccaaacaa tccctggctg
     7981 attggcgcag ctgccagcac aaaaaccagc gccatcagcg tagagaaacc aagtaccatc
     8041 gctgatgaag tgccgaccac gcggcgcagc tgttgcggat tatcatggta ctgggcaacg
     8101 tatttggtta caccgttaaa gatgccagct ccggcaagca cgccgagcac ggtaatcaac
     8161 tggcggaaat ttgccgccag cccaagcccc gccggaccaa atgacacagc cagcaactta
     8221 ccgaccagta acccggcacc aatcttgacc agtgtactgg ccgccgtcca caaggacgct
     8281 tttgccaacg acatatcagg agaagtagtt caacaaagtc gcaattaccg tacgctgatt
     8341 gacgggcgac aggttgtaga acagcggcag gcgcagcagg cgctcgctct ctttggtggt
     8401 gtagcgatct tcaccgtgga actcaccaaa gcgttccccc gcagggcaac cgtgcagcgg
     8461 aatgtaatgg aacaccgcca tgatttccgc ttctttcaga aagttaatca acgcgctccg
     8521 gtcatcaata tcccgcagtt taatgtagaa catatgcgcg ttctgcacgc agccatcggg
     8581 aatcgacggc agctcgatac gcccggcttt cgccagaggc gctaacgcat cgtagtagtt
     8641 ttgccacagc gccagacgtt gctggttgat acgatccgct gcttccagtt gcgcccacag
     8701 gtacgcagct tgcagatcgg acatcaaata gctggagcca atatcgcgcc aggtatattt
     8761 atcgacctga ccacggaaga actggctgcg gttagtgccc ttttcacgga tgatctcggc
     8821 tcgttcgatt aacgctttat cgttaatcag cgtcgcgccg ccttcaccac ccgccgtata
     8881 gtttttggtt tcatggaagc taaagcagcc aatatgacca atggttccca gtgcacgccc
     8941 tttgtaagtg gacatcacgc cctgagcggc atcttctacc acaaacagat tatgcttttt
     9001 cgccaacgcc ataatggtgt ccatttcgca ggccacaccc gcgtaatgga ccggcacgat
     9061 aacgcgcgtt ttgtcggtga tcgccgcttc aatcagcgtt tcgtcgatgt tcatggtgtc
     9121 cgggcgaaca tccacaaaaa cgatttttgc gccacgcagc acaaaggcat tggcggtgga
     9181 gacaaaggtg tagctcggca tgatcacttc atcgccaggc tggatatcga gcagcagcgc
     9241 cgccatctcc agcgaagcgg tgcaggacgg cgtcagtaac actttggcgc tgccaaaacg
     9301 ttgctccagc cactgctggc agcgacgggt aaaaccgcca tcgccacaca gtttgccgct
     9361 acccattgcc gactgcatat agtcgagttc ggttcccacc accggcggtg cgttaaatgg
     9421 aatcatgtga tcacctgtat aaccagtacg cggtgctttc tacattcgca ccactttgta
     9481 tgtatcgttt aagcgcggcg gtgttgccca tttgggtcgc cacccgcaaa gttgttttac
     9541 cgcgagcata cgcccagttt agcgccgttt gcatcagctc agcacctaca ccgcgtccag
     9601 ccagcaggcc aattcgcgca tctgtcgcat tgagttcccg taaagagaca taaccgcgaa
     9661 taccgccgga cgccgcacgt aaaatcagac attgatgatc aaaggtgccg cgcacggcat
     9721 tttcaatcca ctgtgcataa aagcgtctgc tggcgtcagg cgcataccac ggcgcacgaa
     9781 aacggctttg cgcaaatgcg gcgctggcta actgacgtaa tgcgggaata tcggtctctt
     9841 gtgccactac agcaccacta tcactgacat tgttcacggg tagtgccaaa tcaacttcac
     9901 cttctaccag ggagaatccc agctgttgca gggcatccag ttcacccgta tttgatgccg
     9961 caattttggc ctgcacccgt gaccacggcg ctaacacgtc tggcgtcagg agcggtgctt
    10021 cagacgtaat gcgcacgatg gcgctgttaa caccaaagaa ggcgttttcc caggttagtg
    10081 gctcaatact ggcgcggacg ggcacgaagt aactccagca gatattggcc gtagccagtt
    10141 ttcgctaatg aactggcagc acgcttcaca ccctcgtcat cgagccagcc gttacgccag
    10201 gcgatctctt ccaggcaggc aatcttaaag ccctggcgtt tttctactgt ctgcacaaag
    10261 gtactggctt caatcaggct gtcgtgagtg ccagtgtcca gccaggcaaa tccgcgcccg
    10321 agcagttcaa cggtcaggtt gcccgcctcg aggtacatct ggttgatgga ggtaatctcc
    10381 agttcaccac gctccgacgg cttcacctgc tttgcgtact ccacgacttt actgtcgtag
    10441 aaataaagcc cggtcaccgc ccagtttgac ttcggctgtt ttggcttttc ttccagcgag
    10501 atagcgcgga aattgtcgtc aaactccacc acgccaaagc gttccgggtc catcacctga
    10561 tagccaaata ccgtcgcccc ttccgtgcgc gccgcaacat gacgcagctt cggactgaag
    10621 ccctgaccga agaagatgtt atcgcccagc accagacaag aaggttcacc gttgaggaag
    10681 gtttcaccga tgataaaggc ctgcgccaga ccgtccgggc tgggctgttc ggcgtattcc
    10741 agctggatac cgaactcact accatcgccc agcaggcgct ggaaataacc tttatcttcc
    10801 ggcgtagtga tgatgagaat ttcgcggata ccggccaaca tcagcaccga cagcggatag
    10861 taaatcattg gcttatcgta aatcggcaac agttgcttcg atacgccgcg cgtaatcgga
    10921 tgcaatcggg tgccggaacc gcccgccagg ataatacctt tcatgtttgc ctccgctgaa
    10981 aattagcctt tcagacctaa acgctcgccc tgatagctgc cgtcctgcac ctgcttccac
    11041 cagctttcat tagccagata ccactgcacc gttttacgca ttccactttc aaaggtttcc
    11101 tgtggcagcc aaccaagttc acgggcaatt ttcgaagcat caatggcata acgcagatca
    11161 tgccccggac ggtcagcgac aaaggtgatc aagtcacgat agtgcgccac gccgtgcggc
    11221 ttgttcggag ccagttcttc cagcagctcg caaatggttt ccacaacatc gagattctta
    11281 cgctcgttgt gaccaccaat attataggtt tcaccgactt tcccggtggt cgccacgcaa
    11341 tacagcgcgc gggcgtgatc ttccacatac agccagtcac ggatttgctg cccgttgcca
    11401 tataccggca gcggtttacc cgccagcgcg ttgaggatca tcagcgggat cagtttttcc
    11461 ggaaagtggt aaggaccgta gttattcgag cagttggtga tcagcgtcgg cagaccgtag
    11521 gtccgcagcc aggcgcgcac cagatggtcg ctactggctt ttgacgcgga ataagggctg
    11581 ctcggcgcat acggcgtggt ttcggtgaag aaatcatccg tcgagtgcag gtcaccatat
    11641 acttcgtcgg tggagatatg atgaaaacgg aacgctgatt ttttatcttc cgtcagcgta
    11701 ttccagtaag cccgcgccgc ttcaagcaat gtataagtcc cgacaatgtt ggtttcaata
    11761 aacgctgccg ggccgtcaat agaacggtca acatggcttt cggctgccag atgcatgaca
    11821 cagtctggct gatgctcagt gaatacgcgt gccagttctg cccgatcgca gatatcaact
    11881 ttctcaaagg caaagtgctc gctttgcgcg accggtgcca gcgacatcag gtttccggcg
    11941 taggtcagct tatcgaccac taccaccgca tcgctcgttt cgttgatgat ataacgcacc
    12001 agcgccgagc caataaaccc ggcaccacct gttatcagaa tttttctcat cagcgccaga
    12061 ctcctttggc atcgacgaca tactgctgat ggacattgtc gccattgata actttgaact
    12121 gactatgatc gaccagcatc accagcacgt ctgccgttgc cagcgcctcg tcaagctgcg
    12181 ccagagtaca aagcccggtc agttttttcg gcaactggtg gatgttaggc tcaacaacca
    12241 gcgtttcgcc gctgtgccac tgggcgatca gttcagcgat ttccatcgcc gggctttcgc
    12301 gcaggtcatc aatattcggt ttaaacgcca gaccaaagca ggcgattttc agttcactgg
    12361 cgcgtttatc ggtagcagca aggcagtcgg caaccgccgc tttcacctga tcgataaccc
    12421 agaacggttt gtgatcgtta acttcgcgcg cggtacggat aagccgcgcc tgctgggggt
    12481 tctgtgccac gataaaccac ggatcaacag caatgcagtg accgcccacg ccagggccag
    12541 gctgaagaat attaacgcga gggtgacgat tcgccaggcg aatcagttcc cagacgttaa
    12601 tcccctgatc ggcacaaatc agcgacaatt cattagcaaa agcgatattc acatcgcgga
    12661 agctgttttc ggtgagctta cacatttccg ccgtccgcga gttagtgacg acacactcac
    12721 cttcgaggaa aattttgtac agttcgctgg cgcgggtcga acaaaccggc gtcataccac
    12781 caatcacgcg atcgttttta atcagctcga ccataacctg gcccggtaac acgcgttccg
    12841 ggcagtaagc aatgttgacg tccgcctgct cgcccacctg ctgtgggaaa gtgagatccg
    12901 gacgcatctc tgctaaccat tctgccatct tctcggttga ccccaccggc gaggtggatt
    12961 caaggatcac cagcgcgcct tttttcagca ctggcgcaat ggagcgagca gccgattcaa
    13021 cgtaggtcat atctggctca tgatcgcctt taaacggcgt gggtacagca atcagccagg
    13081 catccgcttc aactggcgtc gtgctcgctc gtaaaaaacc gccttctacg gcagttttta
    13141 ctacgctcgc caaatcaggc tcgacgatat ggatttcgcc acgattgatg gtatcaaccg
    13201 cgtgttggtt gatatcgaca ccaatcacct gtttttgccg tgaggcaaac gctgctgctg
    13261 taggcagccc gatgtaaccc agtccgataa cagaaatggt cgcaaaactc atagtgatat
    13321 ccgattattt tttaacgctt ccagaatgcg agagcatgcc tgaccatcac catacgggtt
    13381 atgggcgcgg ctcatagttt gatattcgtt ttcgtctttt aaaagacgcg tcacttcctc
    13441 gacaattcgc tgcttatccg tgcctaccag acgcaccgta cccgccgtca ccgcttccgg
    13501 acgctcagtg gtatcgcgca tcaccagcac cggtttcccc agcgaaggcg cttcttcctg
    13561 aatgccgcct gagtcggtca aaatcagcca ggcgtggttc atcagccaga caaacggtaa
    13621 atactcctgg ggatcgatca gaatgacatt tttcacatgc cccagaatgc gattgaccgg
    13681 ctctctgacg ttcgggttga gatgcaccgg atagacaatc tggatgtcct ggtgcgtggt
    13741 ggcgatgtct gccagcgcgt ggcagatttc ttcaaagcca cgaccgaaac tttcacgcct
    13801 gtgaccggtc accagaatca tctttttatc ggggtcgata aacgggtaat ttgccgccag
    13861 ttctgaacgc agcgtgtcgc tgctcatcac ctgatcacgc acccataaca gtgcatcaat
    13921 gactgtatta ccagtaatga agattcggct atccgcaacg ttttcacgca gcaagttttg
    13981 ccgggaagtt tcggttggag agaagtgata catcgccaga tgcccggtca atgtacggtt
    14041 agcctcttcc ggccacggcg aatagagatc gcccgtgcgt aaaccagcct caacgtgacc
    14101 aacaggaata cgctgataaa acgccgccag gctggttgcc agcgtcgttg ttgtgtcgcc
    14161 gtgaaccagc acgacgtctg gtttgaactc ggcaagaata ggttttagcc cttccagaat
    14221 ccgacaggtt atctctgtca ggccctgtcc tggctgcatt atgttgagat cgtagtcagg
    14281 tacaatggaa aagagtttca gcacctgatc gagcatctcc cgatgctgcg cagtgacgca
    14341 aactttagcc tcaaaaaaag gatcttttgc caacgcatgc accaacggag ccatcttgat
    14401 ggcttccggg cgcgtaccaa atacagtcag tactttcaca tcgattctct tcgaataagc
    14461 ggcgagcgcc tttgcgctcg ccgcagcagt gttgctattt cgagcaacgg cgggttaatg
    14521 cgacaccagc cccgatcagc cccccgacaa tgccccacat aatcatcagg aaggcacgac
    14581 gtgggctatc gcgttttacc ggttcttccg gcgtacgcaa atagcgatag gtctgaaaac
    14641 gcggatccag agttggacca acattcaggg tgtttaacat ggcccgattc tgatcatagt
    14701 cgagatcaaa ggccggaccg acggcctgta aattttccag tcgagcctgg agcattggac
    14761 gcccaagcag gaacatttct gaatcaggta attcctcggc aggcacatct gtcgcactgc
    14821 gcgaaatatt atgctgctca gcaattttca gcgcctgctc aatgctgttc atccggcggt
    14881 cgtagatggc tttcgccacc tcttcctgac gcttcacctg agctttcatc tggatggtgc
    14941 gtgccgccca tccgcctttc agctcctcat tcagatggct ggctgcacgc tggctggcaa
    15001 aagcaacata ctgacgtaac aggttattag cgtcaggcgc ggtttcggca ataagcttca
    15061 cgctgtcatt gaccgcgcgg gtaaagtctc cggggataaa ctggatgttg ttaatcattt
    15121 catccagcaa cgccgcatcg gctttgctgt tgcccaccat ccgctgcttg taatagtcgg
    15181 tttgcagcca gaactctctg cgggtatccc acgaggccag ttgcataaca aactctttat
    15241 aggcttcgtc catgaccgat ggttggtcgg cagaagccat gtttgaacgg acatccaggt
    15301 tacgcaaaaa ttgctgctgc gagtaatatc cccccagcat attcaccgtt ggacgatcgg
    15361 taatcgccgt cgcgctccac tcttgtcgag caaaaaaagt atacgccagc gcgattaacg
    15421 caaacgccag ccccatgcca ataatccata gcttcccagc ccacaaggta cgaaacaacc
    15481 cacgaatatc cagttcattt tcagcgtctt cggccggttt cccaggcatt ggttgtgtca
    15541 tcacatcctc atttatttgg ttaaattggg gctgccacca cgatttctac gcagtctgcg
    15601 ttttacgcgc ttaataaagc gagcaacttt ccaggcacgc ttaatgcaat atccatagag
    15661 gaagaatgct agcaaaaaga gcaccagcat gacccactcc gggacaaaat gagaatattc
    15721 tgccagcacg ccaatggaag cgagcagtgc tgcggcaagg gtaatcagca caaacgcctg
    15781 acgggaagta aacccggcac gcatgatcaa atggtgaata tgctgacggt caggagagaa
    15841 tgggctcatg cctttacgca ggcgacggta cataatcgcc accatatcca ttagcggaat
    15901 ggcgattatc cacaaagcgg taaccgggct gatgggatgg gttttgccct gggtcgtttc
    15961 gagcaggatc cagataacgg taaaaccaat cagcgtactg cccgcatcac ccataaagac
    16021 tttgtagcgg cgacccagga taccaaggtt aagcatgatg tatggcagga tggcggcgat
    16081 catcgcaaag caccagattg cgaggctggt ttgcccgtcg aaccacaaaa tcataccgat
    16141 tgctgcaaac gagacgcagg acaacccgcc cagcaagcca tcaatgccat caaccatgtt
    16201 gaacgcatta atggccgccc agacggcaaa tagcgtcagg aagtaaccaa acggtccgag
    16261 caccatctcc caggagccaa agatataacc caggctactg agataaagtt tgccgaacac
    16321 catcataaca atgccaacag cggcctgtat ggtggcacgg atttttacgc tgatatcaaa
    16381 acggtcatcc agcgcgccaa tgaaaacaag cacaccggca caagcgagat agagagatgc
    16441 atgcggaata tagtaatcga caattccgaa cgtgaagcaa atccctgcgt aaaccgaaat
    16501 ccccccaacg agaggtatca atccctggtg acgtttgcgg aagtttggtt tatccactaa
    16561 accgactttt tttgccactt tacgggcaaa aaacagaaac agtgtcgtga ataaaaaaat
    16621 actgatgaga tcagtactca ctgtcagtaa attcacaatg catgctctca gagaaaatta
    16681 ttagcagaag tataaccacg aagaccttta ttcagaaggg aaagtccgtt ccaaacccac
    16741 cagtcctgtg caaaaattaa ccatttgata tgcaaattgc ataacatgcc agcaaattcc
    16801 aggaattttc ctaacatcgc cagcgcggca ttaagattac agatataaaa gcaaaacgcc
    16861 acgtaaacac gtggcgtttt tggcataaga caaatttatg agcgtttcat catttcgaag
    16921 aaatcgtcat tggtcttagt catggccagt ttattaatga ggaattccat tgcatcgatt
    16981 tcgcccatcg ggtgaatgat tttgcgcagg atccacattt tctgcagttc ttcctgagtc
    17041 gtgagcagct cttctttacg ggtaccggaa cggttgtagt cgatagccgg gaagacgcgt
    17101 ttttcagcga tcttacgaga gaggtgcagt tccatgttgc ctgtaccttt aaactcttcg
    17161 taaataactt cgtccatttt agaaccggta tcgataagcg ccgtcgcgat aatggtcagg
    17221 ctgccgccct cttccacgtt acgcgccgca ccgaagaagc gtttcggacg atgaagggca
    17281 ttggcgtcca caccaccggt caacacttta cctgacgccg gaacaacagt gttgtaagcg
    17341 cgcgccagac gagtgatgga gtcgagcaga atgataacgt ctttcttgtg ctcaaccagg
    17401 cgtttggcct tctcgatcac catttccgca acctgaacgt ggcgagatgc gggttcgtca
    17461 aaggtagaag caacaacttc accttttacc agacgctgca tctcggttac ttcttccgga
    17521 cgttcgtcga tcagcagaac catcagcaca caatccgggt ggttgtaagc aatgctctga
    17581 gcaatgttct gcagcagcat agttttaccg gctttcggcg gtgccacaat cagaccacgc
    17641 tgaccacgac cgataggtga tgccagatcc agtacgcgag cggttaaatc ttcagtagaa
    17701 ccgttaccac gttccatacg cagacgagag tttgcgtgca gcggggttaa gttctcaaag
    17761 aggattttgt tgcgggcgtt ttcaggtttg tcgaagttaa cttcgttaac tttcagcagc
    17821 gcaaaatagc gttcaccttc tttcggcggg cgaatcttac cagagatggt atcaccagtg
    17881 cggaggttga aacggcggat ttggctaggg gaaacgtaga tgtcatcagg accggcgagg
    17941 taggagctgt ctgcggaacg gaggaaacca aatccatcct gcaatatctc cagtacgcca
    18001 tcaccaaaga tatcttcgcc actctttgcg tgctgcttca ggatggcaaa aataatgtcc
    18061 tgcttacgca tacgagccag gttttccagc cccatatttt cgccgagagt gatcagctca
    18121 gaaaccggcg tattctttaa ttcggtaaga ttcatagtgg tgtgagttct taaacttggg
    18181 gtaaatctcg aacttaatgt tgtgaatggt atggcaggat catccatgcc tgtttacggt
    18241 catcgactca tgtcttttcg ctgcctggtc acaggagagt acgcagaact gaaacgacaa
    18301 gacggattga gtgacgagcc agaaatctgt tcacttcgca tttaagataa aacgggaagc
    18361 gttggaaatt acaagattca aacttaataa ggtatgttta atacgaagtc aacactaagt
    18421 tagcatgact cacgccgggc gtccagtttt tagcgacggg gcacccgaac atgaaattcc
    18481 cttacgccag gttagcgtcg aggaactctt tcaactgacc tttagacagt gcacccactt
    18541 tggttgccgc cacttcaccg tttttgaaca gcagcagagt cgggatacca cggatgccat
    18601 atttcggcgc agtgccaggg ttttgatcga tgttcagttt tgcaacggtc agtttgccct
    18661 gatattcgtc agcgatttca tccagaatcg gggcgatcat tttgcacgga ccgcaccact
    18721 ctgcccagaa atcgacgagg atcgccccgt ccgctttgag tacatccgtg tcaaaactgt
    18781 cgtcagtcag gtgaataatt ttatcgctca tatataactc cacaggaata agcctggcgt
    18841 gttggtttcg ttgttggtgt aacattaacc aactaaaggt tgactttatt tcaccggata
    18901 cgctttcgta aagcaatagt aagctgatat tctaccacac tatgagcaaa acacatttaa
    18961 cagaacagaa gttttccgac ttcgccctgc atccgaaggt tgtagaagcc cttgaaaaaa
    19021 aagggtttca taactgtacg cccattcagg cactggccct tccgctgacg ctggcgggtc
    19081 gtgacgtagc cgggcaggcg caaaccggta ccgggaaaac gatggcgttt cttacgtcaa
    19141 cgtttcatta tcttctctct catcctgcga ttgccgatcg caaggtgaat caaccacgtg
    19201 ccttaattat ggcaccgacg cgtgaacttg ccgtgcagat ccatgccgac gcagaaccgc
    19261 tggcggaagc taccggcctg aagctgggtc tggcttacgg tggtgatggc tacgacaaac
    19321 agctgaaagt gctggaaagc ggcgttgaca ttctgattgg cactacgggg cgtttaattg
    19381 actacgccaa gcagaaccac attaacctcg gtgccattca ggtggtggta ctggacgaag
    19441 ccgatcgcat gtacgatctg ggctttatta aagatatccg ctggctgttc cgccgtatgc
    19501 cgcctgcaaa ccagcgcctc aacatgctgt tctccgccac gctttcgtac cgggtacgtg
    19561 aactggcgtt cgagcagatg aacaatgccg aatatattga agtggaaccg gaacagaaaa
    19621 cgggccaccg tattaaagaa gagcttttct acccttctaa cgaagaaaaa atgcgtttgc
    19681 tgcaaacgct gatcgaagaa gagtggccag acagagcgat tattttcgcc aataccaaat
    19741 accgttgtga agagatctgg ggccacctgg cggcagatgg tcatcgtgtc ggtttgttga
    19801 caggcgatgt cgcgcagaaa aaacgtctgc gtattcttga tgaatttacc cgtggcgatc
    19861 tggatattct ggttgccacc gacgttgccg cgcgtggttt gcatattccg gcagtgacgc
    19921 acgtctttaa ctacgattta cccgatgact gtgaagatta cgttcaccgt attggtcgta
    19981 caggtcgcgc aggcgcaagc ggtcactcta tcagcctggc gtgtgaagag tatgcattga
    20041 atttgcctgc tattgagacc tatattggtc actcaattcc ggtaagcaaa tacaatccgg
    20101 acgcattgat gaccgatctg ccaaaaccgc tgcgcctcac gcgcccacgt acaggcaatg
    20161 gtccgcgtcg tactggcgct ccgcgtaatc gtcgtcgttc aggttaaata aaatgtcata
    20221 ctcaaaatca ttcaaactgt atcaagatgg caagagaatg aatcccgatg agcatactgt
    20281 agtaagtgat tcaggtgaaa gaatgcagcc aacacagaga cagattgaag gatgaagagt
    20341 atgggttcca cctcgtcgct gtatgcagcc attgatctcg gttcgaatag ttttcatatg
    20401 ctggttgtgc gcgaggtggc tggaagcatc cagacgctga cgcgaataaa acgcaaagtg
    20461 cgtctggctg ctggcctgaa tagcgaaaat gccctgtcta atgaagcaat ggagcgcggt
    20521 tggcaatgtc tgcgcctgtt tgctgaacgt ctgcaagata tccctccctc gcaaattcgc
    20581 gttgtcgcta cggcgacatt acgtcttgcc gtcaatgcgg gtgattttat tgccaaagcg
    20641 caggaaatcc tcggttgtcc ggtacaggtg atcagcggtg aagaggaagc acgtctgatt
    20701 tatcagggcg ttgctcacac aactggcggt gccgatcagc gcctggtggt ggatataggc
    20761 ggtgccagta ctgaactggt aaccggcacg ggtgcacaaa ccacctcgtt gttcagcctg
    20821 tcgatgggct gcgtcacctg gctggaacgc tattttgccg atcgtaatct ggggcaggaa
    20881 aattttgatg ctgcagaaaa agcggcacgc gaagtgttac gtccggttgc cgatgaatta
    20941 cggtatcacg gctggaaagt gtgcgttggt gcttccggca ccgtgcaggc gttacaggaa
    21001 atcatgatgg cacaggggat ggatgaacgc attaccctgg aaaagttgca gcaattgaaa
    21061 cagcgagcca ttcattgcgg tcggctggag gaactggaga ttgacgggct gacgctggaa
    21121 cgtgcgttag tgttcccgag tggtctggcg atcctgatcg ccatttttac cgaactgaat
    21181 attcagtgta tgaccctggc gggcggtgcg ctgcgtgaag gcctggtcta cggcatgttg
    21241 catctggccg tcgagcagga tattcgcagc cgtacgctgc gtaatattca gcgccgcttt
    21301 atgatcgata ttgatcaggc gcagcgcgta gccaaagttg cggctaactt cttcgatcag
    21361 gtggaaaagg aatggcatct tgaagcaata agccgcgatt tgctcatcag cgcctgtcag
    21421 cttcatgaaa tcggcctgag cgttgacttt aaacaagcgc cgcaacacgc tgcttatctg
    21481 gtgcgtaatc tggatcttcc cggttttacc cccgcacaga aaaaattgct ggcgacgcta
    21541 ctgctcaacc agactaatcc ggtcgatctc tcatcgctgc atcagcaaaa tgccgtacct
    21601 ccgcgcgtcg cagaacaact ctgccgttta ctccgcctgg ccatcatttt tgccagccgt
    21661 cgccgtgacg atctcgtgcc agagatgaca ttacaggcta accatgaact gttgaccttg
    21721 acgcttccgc aaggttggct aacccaacat ccgctgggta aagagattat tgctcaggaa
    21781 agccagtggc agagctatgt ccactggccg ctggaagtgc attaatgagt aagtgccgga
    21841 tgcgatgctg acgcatcttt tccggccttg attatttccc tcgttttgcc gccatcatcg
    21901 ctttcagatt cgccagatgg ctttgccctt tctgcatccg ttcttcggcg ctgaccactt
    21961 tgcgctcctg ttcccaaatc agatcatcct gcggcagctc cagcaaaaag cggctcggct
    22021 ccgggcgcac caactcgccg tactgacggc gttctttaca cagcgtaaag gtcaattcct
    22081 tctgggcgcg ggtaatgccg acataggcca gccgccgctc ctcatcgata ttatcttcat
    22141 cgatgctgct ctggtgcggc aaaaaccctt cttccatacc gaccatgtag acataaggaa
    22201 actccagccc ttttgacgcg tgcagagtca tcagttgcac ctgatccagc tcttcttcac
    22261 tctcaccacg ctccatcatg tcgcgcaaag taaagcgcgt caccacctgg gtgagcgtca
    22321 tcggctcatc cagttcgctg ccttccagca tctccgtcat ccagctaaac agttggttga
    22381 cgttcttcat gcgcatttcg gcggctttcg ggctgggcga tgtttcgtac agccaggatt
    22441 cataatccat gccgtggatc agatcgcgca ccgcggcaat cggctcccgc tccgccagac
    22501 gctggatttc tgccaaccag tgagtaaagc gggtcaatgc ttcataacca cgtccgctaa
    22561 gcgtctgact caggcccata tcaaagctgg cggtaaacat gcttttattg cgcgtcatcg
    22621 cccactcacc cagctttttc agcgtcgccg ggccaatctc tctcttcggc gtgttaacga
    22681 tacgcagaaa tgcgctgtca tcgtccgggt tagtcagcac gcgcagataa gccagcaagt
    22741 ccttgatttc aggacgagag aaaaacgacg taccaccaga aattttgtac gggatgcggt
    22801 tttgcatcag gaatttttca aacactcgcg actgatggtt accgcgataa agaatagcgt
    22861 aatctttgta ctgcgtttta ttgacgaagt gatgcgcaat cagttcacca gtgacgcgct
    22921 cggcttcatg ttcttcgtta ttcgcgctta aaactttaag ctctgtgccg taacccagtt
    22981 cggagaacag acgcttctca aagacgtgcg ggttattggc gatcaggatg ttcgccgctt
    23041 tcaaaatacg tccggaagag cggtagttct gctcaagctt aatcaccttc agcgccggaa
    23101 aatcctgact cagcagcacc aggttttgtg gacgtgcgcc gcgccaggag tagatcgact
    23161 ggtcgtcatc gcccaccacg gtaaagcgcg cgcggctgcc caccagcagt ttcaccagtt
    23221 catactggct ggtgttggtg tcctgatact catccaccag cagatagcga attttgttct
    23281 gccagcgctc gcggacttct tcattgcgtt gcagcagcaa cgtcggcaat aaaatcagat
    23341 catcgaagtc gagaacattg cacgctttca ggtgtgcatc atacagccca taacaatggg
    23401 caaaaatacg atcccgctcg ccctttgcct ctgccgccgc ctgcgccgga gttttgagat
    23461 cgttcttcca gttggagatg gtcgaaatca gctgttgcag gagaactttg tcatcttcaa
    23521 tcagcccctc ggtcaactct ttaagcaaag caagctgatc ggtatcgtca aacagcgaaa
    23581 agttcgcctt catcccaagc gccgcatact cgcgtttgat gatatccaac cccagcgtgt
    23641 gaaaagtgga gatcatcaac ccgcgcgcct ctttgcgacc cagcgtctgc ccgacacgct
    23701 ctttcatctc gcgcgccgcc ttattggtaa aggtcaccgc cgcgatatgt cgcgcctgat
    23761 aaccgcaacc gcggatcaga tgggcgattt tattggtgat cacacgagtt ttaccggaac
    23821 ccgcgcccgc cagcaccagg caggggccgg taacgaattc gacagcttgt tgttggccgg
    23881 ggtttagacg cataggtgta ttgctcaatc ttcgaacggg gggaggattg tagcagaatc
    23941 gcatttgaat gtgaccgcgt gtaagccgct aaccggttgg ctgcggatgt ttaaatcaga
    24001 gagagaagac cgaaatcgga gttcatcgtg aatccattag acttagaaaa tatcgggtcg
    24061 taacgttgtg ttattacttt ttcagagttt actaaggaga ggtttactcg tggcgattaa
    24121 tttcagccct aaggttggtg aaatacagga atgcaacttt ggtaactatc ctgttatcca
    24181 gaatggacag ttcagcacca cgtattacga tggccgcatt cctcctgaaa tgataaaaaa
    24241 caggttagtt gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt
    24301 atcaaccact cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa
    24361 tgttatcaat ggcctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctggt
    24421 acaacaagtc agtcgaaacc gattaaatag agcaaggact tacagagggt atcttaatca
    24481 atgtttgcca catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc
    24541 catatcgctc ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata
    24601 gaggccctaa gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc
    24661 catatatgta ccagcccctc cctgactgac aattacgtta gcatctgcgg atcatgctaa
    24721 aatcgccgcc tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact
    24781 gcatatcctt gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg
    24841 tgccgatttc ggcaagctgg cgaagaaaca ctctatttgc ccgtcaggca aacgcggcgg
    24901 tgatttaggt gaattccgcc agggtcagat ggttccggcg ttcgataaag tggttttctc
    24961 ttgtccggta ctggagccga ccggcccgct gcacacccag ttcggatatc acatcattaa
    25021 ggtgctgtac cgcaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc
    25081 cctctccctg tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaacagc
    25141 aatacgtttc atatctgtca tatagccgcg cagtttctta cctacctgct caatcgcatg
    25201 gctgcgaatc gcttcgttca catcacgcag ttgcgcgtta tctaccgcgc cttccggaat
    25261 agctttaccc aagtcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca
    25321 agcgtaagag aacagatagt taccgtactc agcggtatca gagataacca cgttcatttc
    25381 gtacagacgc ttacgggcga tggtgttggc aatcagcggc agctcgtgca gtgattcata
    25441 atatgcagac tcttcaatga tgccggaatc gaccatggtt tcgaacgcca gttcaacgcc
    25501 cgcttttacc atcgcgatca tcagtacgcc tttatcgaag tactcctgct cgccgatttt
    25561 gccttcatac tgcggcgcgg tttcaaacgc ggttttgccg gtctcttcac gccaggtcag
    25621 cagtttctta tcatcgttgg cccagtccgc catcataccg gaagagaatt cgccggagat
    25681 gatgtcgtcc atatgtttct ggaacagcgg tgccatgatc tctttcagct gttctgacag
    25741 cgcataagca cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc
    25801 ctgcttcagc gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc
    25861 cggatcggtg ccctcttcca ccagcttgtc gaagcacagc agcgagccag cctgcaacat
    25921 accgcacagg atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga
    25981 ttccagcacg cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat
    26041 accttcgcct ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc
    26101 acgtttgtac tcttcacgca cttcggtgcc agggcatttc ggcgcaacca tgactacggt
    26161 gatatcttta cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag
    26221 cgccgcgccg tctttcatca gtggctgtac ggtgcgcact acatcagagt gctgcttgtc
    26281 cggcgtcagg ttaaccacca gatccgcctg cgggatcagt tcttcgtaag taccaacttt
    26341 aaaaccgttt tcggtcgctt tacgccagga cgcgcgcttc tcggcaatcg cttctttacg
    26401 cagagcgtag gagatatcca gaccagaatc acgcatgttc aggccctggt tcagaccctg
    26461 tgcgccacag ccgacgatga ctactttttt accctgaagg tagctcgcgc catcggcgaa
    26521 ttcatcgcgc cccataaagc gacatttgcc cagctgtgcc agctgctgac gcagattcag
    26581 tgtattgaag tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt
    26641 cgcgtttgcg agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt
    26701 cacaacgtca cattgcaatt tttgcaacgt caacatcgag ggctgcgcca ttggatttac
    26761 gcgatctgaa aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg
    26821 cgatgcacgt tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg
    26881 gtcagccgct atttgtgcgc gataaccgca cggtaacgct gacggaagcg ggcgaagagc
    26941 tgcgcgtttt cgcccagcaa acgctgttgc agtatcagca attgcgccac accatcgatc
    27001 agcaagggcc gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca
    27061 gccatctgcc gccgattctg gatcgcttcc gcgcggaaca cccgtcggtg gagatcaaac
    27121 tcaccactgg tgatgcggca gatgcaatgg aaaaagtggt cacgggagaa gcggatctgg
    27181 cgattgccgg gaaaccggag acgctacccg gcgctgtggc gttttcgatg ctggagaatc
    27241 tggcagtagt gctgattgcg cccgcgctgc cctgcccggt gcgtaaccag gtttcggcag
    27301 agaagccgga ctggtctaca gtgccgttta tcatggccga tcagggaccg gtgcgtcgcc
    27361 gcattgaact gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg
    27421 gcgggcatga agcgatggta tcgatagtgg cgctcggctg tggtgtggcg ctgttgccgg
    27481 aagtagtgct ggaaaacagc cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca
    27541 gcgatgaaaa aacgccgttt gaactgggcg tgtgtgcaca aaaaaagcgg ctgcatgagc
    27601 cgctgattga ggcgttctgg aggattttgc cgaaccacaa atgacgttgt cgcgcgggta
    27661 ggcctgataa gcgaagcgct atcaggcatt tttccctaac ccgccaaaaa gaacctgaac
    27721 gccgggttat tggtttcgtc gtggcaatcg tagcccagct cattcagccg ggtttcgaaa
    27781 tccggttcat ggtcgccaag ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg
    27841 ctgcgatagt ggaacaaaga aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc
    27901 agcgcgcccg gtgattccgg gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac
    27961 ggacgcccgc cgaccatata gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc
    28021 accacgctgt agccgccgtc gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg
    28081 cggctcaggc gcacgccgac aaagatgcag gcgtttttgg catcggcaaa acggtagttg
    28141 aactcggtga ccgatcgccc gccaagcagt tggcagaatt tgaggaagct gcctttttct
    28201 tccggaatgg tcaccgccaa caacgcttca cgctgttcgc ccagttcgca gcgttccgag
    28261 acgtagcgca ggccgtggaa gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg
    28321 ccgcgaatgt tgtgctgggc gatatatttt ttcattcccg ccagcgccag cgcgccagag
    28381 ggttccgcca ccgcgcgcac atcttcgaat aaatccttca tcgccgcaca gatcgcatca
    28441 ctatcgacgg tgatgatgtc gtcgagatac tcctggcata aacggaaggt ttcgtcaccg
    28501 atgcgtttta ccgctacgcc ttcagcaaat agccctacgc gcggcagatc aaccggatga
    28561 cccgcatcca gcgctgcttt caggcaggcg gagtcttccg cttctacggc gatcactttg
    28621 atttgtggca tcagttgttt gatcagcacc gcaacgcccg ccgccagacc gccgccgccg
    28681 actggcacaa atacgcggtc gagatgggcg tcctgctgga gcagttccag cgccagcgtg
    28741 ccttgcccgg caatcaccat cggatggtcg aacggcggca cccaggtgaa cccctgctgc
    28801 tgtgacagtt cgatcgcttt agctttcgct tcgtcaaagt tcgcgccatg aagcagcact
    28861 tcgccgccga atccacgtac cgcatcgact ttgatgtcgg cggtggcggt tggcataacg
    28921 atcagggcct tcacgcctaa ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc
    28981 gcagaagcag tgatcacgcc gtgcgctttt tgttcttccg tcaggcccgc catcatggcg
    29041 tatgcgccgc gcagcttaaa gctgtgcact ggctggcgat cttcgcgctt caccagaatg
    29101 acgttatcaa gacgcgacga cagtttttcc attttttgta gcggcgtaac ctgcgccgcc
    29161 tcgtaaaccg gcgcgcgcag cactgctctt aaatattcgg caccttccgg agcaccggac
    29221 aggggttgcg agtcagccat tattaacccc ccagtttcga tttatcgcgc accgcacctt
    29281 tgtcggcgct ggttgccagg ctggcgtagg cgcgcagggc aaaggaaacc tgacgttcac
    29341 ggtttttcgg cgtccaggct ttgtcacctc gagcgtcctg cgcttcacga cgcgccgcca
    29401 gttcggcatc gcttacctgt aactgaatgc cacggttcgg gatgtctata gcgatcaggt
    29461 caccatcttc aatcaggcca atgctgccgc cgcttgccgc ttccggtgag acgtggccga
    29521 tggaaagacc agaggtgccg ccagagaaac gaccgtcggt gatcagcgca caggctttac
    29581 cgagacccat tgatttcagg aagctggttg ggtagagcat ttcctgcatg ccagggccgc
    29641 ctttcgggcc ttcataacgg atcaccacca catcgcccgc gacaactttg ccaccgagga
    29701 tcgcttctac cgcgtcgtcc tggctttcgt acactttcgc cgggccggtg aatttgagga
    29761 tgctgtcatc gacgcccgcg gttttaacga tgcagccgtt ttctgcgaaa ttaccgtaga
    29821 gcaccgccag gccgccgtct ttgctgtagg cgtgttccag cgagcggata cagccattgg
    29881 cgcgatcgtc atcgagagaa tcccaacggc aatcctgcga gaatgcctgt gtggtacgaa
    29941 tgcctgcagg acctgcgcgg aacatatttt ttaccgcgtc atcctgggtc agcataacgt
    30001 cgtattgttc cagcgtttgc ggcaacgtca ggccaagtac gtttttcaca tcacggttca
    30061 gtaaccccgc gcgatccagt tcgccgagaa taccgataac accaccagca cggtgaacat
    30121 cttccatatg gtatttctgg gtgctcggcg caactttaca cagctgtgga accttgcggg
    30181 aaagcttatc gatatcactc atggtgaagt cgatttccgc ttcctgcgcc gccgccagca
    30241 ggtgaagtac ggtgttagtc gatccaccca tcgcgatatc cagcgtcatg gcgttttcaa
    30301 acgccgcttt gctagcaata ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac
    30361 gtttggtcaa ttcaacaatg cgtttaccgg cattgaggaa caactgctta cggtcggcgt
    30421 gggttgccag cagcgagccg ttgcccggct gcgacagacc cagcgcttcg gtcaggcagt
    30481 tcattgagtt agcggtaaac atcccggagc aggagccgca ggtcggacac gcggaacgtt
    30541 caacctgatc gctctgggag tcagaaactt tcgggtctgc gccctggatc atcgcatcaa
    30601 ccagatcgag tttgattatc tgatcggaca gtttggtttt cccggcctcc atcgggccgc
    30661 cggaaacaaa gatcaccgga atattcaggc gcagggaagc catcagcatc cccggggtga
    30721 ttttgtcgca gttagagatg cagaccatgg cgtcggcgca gtgggcgttg accatatact
    30781 caacggaatc agcgatcagt tcgcgagatg gcagtgaata aagcatcccc ccgtggccca
    30841 tggcaatccc atcatccacc gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt
    30901 caatttgttc ggcgaccagt ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt
    30961 gggtgaacga gttcacaacc gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg
    31021 tggcgcgcca cagcgcacga gcacccgcca tattacgacc atgagtggtg gtggcggaac
    31081 ggtacttagg catactttat ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc
    31141 cgtcccattt tttgtattta ttgattaact tgatctaacc agccccattt atcttcggtt
    31201 tcgccagtga agaggccgaa gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg
    31261 ccttcgccaa cctgaatacc atctacactg cgcactggcg tgatttctgc cgcagtaccg
    31321 gacataaaca cttcatccgc caggtacagg gattcgcgcg acagaacctg ctcacgcact
    31381 tcaattccca gctctttcgc cagtttgatg atggcatcac gggtaatacc cggcagcgcg
    31441 gaggaggtga acggtggggt gaacagcaca ccatctttca cttcaaacag gttttcgcct
    31501 gcgccttcag agatataacc gttcacatcc agcgcgatac cttcctgata accgtggcgg
    31561 cgcgcttcgc tacccaccag cagggaagag agatagttac caccggcttt tgccgcggtt
    31621 gggatggtgt ttggtgctgc gcggttccag gaggaaacca tcgcatcgat cccctgctcc
    31681 agcgcttctg cgcccagata cgctccccac gggaaagcgg cgataatcac atcggttgag
    31741 tatcccgctg gcgggtttac acccatgcca acatcaccaa cgaagatcag cggacggata
    31801 taggcgctgg tgagattgtt tttgcggatc acgtcacgac aagcttccat cagctcatca
    31861 atgctctgcg aaaccgggaa gcgatagatt ttggcggagt catgcagacg ctgcatatgc
    31921 tcacgatggc ggaatacaac cggtcctttg tgcgagtcgt agcaacggat gccttcaaaa
    31981 accgaagtgc catagtgcag cgcgtgcgac atcacatgca ccttagcgtc ttcccagcga
    32041 accatctccc cattgaacca aatgtaatca gctttcttcg tggtcatttt tatattcctt
    32101 ttgcgctcag gcgcggattt gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc
    32161 gacgtccacc agtttattta actgactaaa cagtaagtcg accgaccgtg ggctggcaac
    32221 ggtcaattcg atatttatat tttgtgcatc gctggcggca gccatattca ttgagcagac
    32281 gtggaaacca cgatgacgca ccacgcgtaa aacacgttct aaggtttccg gattgaagcg
    32341 agccgataca ttgacctgat gttgcatcat gataatttct ccaacatttc tgaattactg
    32401 gcgccaggcg gcaccagcgg ccagacgttc tcaagttcgt cgattgagac atgaagcagg
    32461 tatggcccat cactgttcag catggtgtcg agtgccgctt caacctggtc tttacgggtg
    32521 atgtgttggc cagggatgcc gaaggcgctg gctaacatga ggaaatcggg gttatcagta
    32581 agggtggttt cgctgtatcg ttcctgaaaa aacagttgct gccattgtcg aaccatccct
    32641 aaccgttggt tatcgagtaa gacgattttc aacggtaact gcttgcgttt tacggtgccc
    32701 agctcttgca cattcatcat gaaagagccg tcaccggaga tacagacgac agtatcgttc
    32761 ggtcgtgcga cttgtgcgcc aaccgccgct ggtaaaccga aacccatggt gcctaagccg
    32821 ctggaggtaa tgaaattttc cgggcgagtg tgtgcgatgt gctgcgcggc ccacatctgg
    32881 tgctgcccca catctgtggt cacgacgcaa tccgcaggtt tacgatccga cagttgtttt
    32941 aacaacaacg gcgcgtagat agcgtcaccg ggatggtcgt aacgccaggt atgttcatca
    33001 cgcagctgcg cgcagtattg ctgccagtca ttgatattta acggctgctg taatgctggt
    33061 aacagagcat ttaaatcacc ttgtaatgcc acatgtgcct gacgcagctt gttcatttct
    33121 gccgggtcga tatccatatg gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg
    33181 gtcacccggt catcaaaacg tgcacccacg gcgatcagca agtcgcactc ctgcaccgcg
    33241 aagttcgccg ctttggtgcc atgcattccc agcatgccca ggtagtacgg ataatctgct
    33301 tcaactgcgc ccagcccttt cagcgtgcag gtggcaggca tttttgtggt agcgagaaat
    33361 tctcgtaaag caggaactgc ctgcgccata cccacgccac caccaacgta cagcatcggt
    33421 ttttgcgctt ttgccagcat ctggcgcgct tgctcaactt cggcatgtgg gaaagtcact
    33481 tcgttttcaa cggtggtgaa ccacggttcc aggtcaccgc tggctagctg gatatctttt
    33541 gggatatcga ccagaaccgg accaggacga cctgagctgg caacgtcgaa tgcttcagcc
    33601 ataatgcgcg gcaactcttc cagcgactgc accagaaagc tgtgcttggt acaggctaac
    33661 gacaatccca ggacatccac ttcctgaaat gcgtccgtgc cgataaacgg tgcggacact
    33721 tgaccggtga tggcaacaac agggatagaa tctaacagtg cgtccgcaag cccggttatc
    33781 aggttggttg cgcccggacc agacgtggcg atacatacgc cagttttgcc ggtagcacgg
    33841 gcataaccga tagccgccat tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg
    33901 ccgccgtcat acaatgcatc gtaaaccggc ataattgcgc cacccggata accgaaaacg
    33961 gtgttcacac cctgtgcccg caacgcatgt accacccact gtgcgccatt catagttagt
    34021 tccccgtcct gaatcttgag aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg
    34081 ttatattttt aaggtcaaaa aaaacccccg gacctttcgg tgcgggggtc ttagttcgtt
    34141 aaggcttgat ctctaagcct ttcctcgtcc aagtgcagcc ccgcacggtg ggataataat
    34201 caccaccacg ctaatcacga ccaggctaat cactcgtaga agggctgtca ttttgtcttt
    34261 tcttgcatct tgttcgaagg aatgcctaaa gagttaccat agattttgcg aatagcacaa
    34321 gatatttttt ggccgatttt tatgatgcgt ttaataattt caatagaaat tgttgttttt
    34381 taaaaagaaa atagtgagtg aaaaaaattc aatttcagta tgaatgttgt tcccttcctc
    34441 gtagttcatc ctttgtccgc tgcatttttg cgagcatcat tccaccgcca gaacaactca
    34501 acttcacttg cacaaataaa caggaatcag ccactaaaat tcacgaatta gcgggctaca
    34561 gcagcgatat cttatgcaca taatagtgac tcaaggaggg cttatgtcac tgtcaattgt
    34621 tcatacccgc gcagccctgg gagtaaatgc gcccccaatc actgttgagg tacatatcag
    34681 taaaggtcta cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg
    34741 cgatcgcgtg cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac
    34801 catcaacctg gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc
    34861 cattgcgttg ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt
    34921 agtcggagaa ctggcgctta caggcgctct gcgtggcgtt cccggcgcaa tctccagtgc
    34981 aactgaagct attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt
    35041 ggggctaatt aacggtgaag gatgcctgat agccgatcat ctgcaggctg tctgtgcgtt
    35101 tctggaaggt aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct
    35161 acaacatgat ctcagtgatg ttgtcggtca ggagcaagga aagcgaggac tggaaattac
    35221 cgccgctggg gggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct
    35281 cgccagccgt attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc
    35341 tgcgatatta agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt
    35401 ccgctcaccg catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg
    35461 gccaggtgaa atttcgttgg cgcataacgg cgtgcttttt cttgatgagc tacctgaatt
    35521 tgaacggcgt acactggatg ccttgcgaga gccgattgaa tccgggcaga tccatctttc
    35581 acgcacacga gccaaaataa cctatccagc ccgtttccag cttgttgcgg cgatgaatcc
    35641 cagccctacc ggacattatc agggaaacca taaccgctgc acgccagaac agacattacg
    35701 ttatctcaac cggctctcgg ggccctttct cgaccgcttc gatctctcac tggagatccc
    35761 attaccaccc cccggcattt tgagtaaaac ggtagtgccg ggagaaagca gcgccaccgt
    35821 taaacaacgc gtaatggccg ccagagagcg ccaatttaag cggcagaata aactgaatgc
    35881 ctggctggat agtccggaaa tacgccaatt ctgcaaactt gagagcgaag atgcgatgtg
    35941 gttggaagga acactgatcc atctggggtt atcgattcgt gcctggcagc ggttattgaa
    36001 agttgcacga accattgctg atattgatca gtctgacatt atcacacgtc agcatttgca
    36061 ggaggcagtt agctatcgtg cgattgaccg tttgctcatc catctgcaaa aactactgac
    36121 ataaaaaaag ggcatttcgc cctttttatt aatcatcaga atcggtgtag tcttcagcac
    36181 cttcaacctg cggtttaccg ccggaaaggg tgtgaaaacg ttttggacgc ttaatacgcg
    36241 tcatatactt ggaccacacg cgttctgctt ctgtcactgg ctcacgttcg ccacggcata
    36301 ctgctacgaa gagtttctct tcctcggtaa ccggctcgcg tttgccaaga tccaactcat
    36361 tgaaggcata accatgacgc tcaagcagtt gtgcctcttt gatggtgaaa tcaccatgac
    36421 gagagaatcc acgtggataa tgtttattgt cgaaatatcg attagtcgtc gtaaagcttt
    36481 ccgccatcct gcacgctcct aattctttga ccgagctagt tatggcgcgg agtattagtt
    36541 acgcttgaca gagtgtaaaa caaaacattt aaatcataac gacaaataat tttgcggaga
    36601 gcactgtgga tacggaattg ttaaaaactt tcctggaagt tagccgaacg cgtcactttg
    36661 gtcgagcggc tgaatcgctc tatctgaccc agtcagcagt gagctttcga atcagacaac
    36721 tggaaaatca actgggtgtg aaccttttca cccgccacag aaacaatatc cgtttaaccg
    36781 ctgccggtga aaaactactg ccttatgcag aaacgctcat gagcacctgg caggccgccc
    36841 gcaaggaggt ggcgcatacc tcacgacata acgagttttc tatcggtgcc agcgcctcat
    36901 tgtgggaatg tatgcttaat cagtggctgg gacgtttgta tcaaaatcag gatgcccata
    36961 caggcttaca gttcgaagcg cgaattgccc aacggcagtc tctggtaaaa cagctgcatg
    37021 aacgccagct tgatcttctt attaccactg aagcgcccaa aatggacgaa tttagtagtc
    37081 agttgctggg atatttcact ttagcgcttt ataccagtgc cccttcaaaa ctaaagggag
    37141 atcttaatta tctgcgactt gagtgggggc cagattttca acagcatgag gcaggtttga
    37201 tcggtgctga cgaagtgccc attctgacaa ccagttctgc tgaactggca cagcaacaga
    37261 ttgcgatgct taatggttgc acctggctac ccgtcagctg ggcgcgtaaa aaaggcggcc
    37321 tgcataccgt tgtcgatagc acaacacttt cacggccgct ttatgccata tggctgcaaa
    37381 atagcgataa aaatacgttg attcgcgatc ttttgaaaat taacgtgctg gatgaagtgt
    37441 attaatatga atggctggca aggatgccgg tagaaggatt tacttcggag agggttattt
    37501 cagataaaaa aaatccttag ctttcgctaa ggatgatttc tggcaggggc ggagagactc
    37561 gaactcccaa cacccggttt tggagaccgg tgctctacca attgaactac gcccctaatt
    37621 agggtggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg caggtattct
    37681 aaccgactga actaccgctc caccgaattc ttttacaacc accggtttta tgaccggctt
    37741 ac
//
LOCUS       DAZGTR010000033        37486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGTR010000033 DAZGTR010000000
VERSION     DAZGTR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.033"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            261..1634
                     /gene="aroP"
                     /locus_tag="ACHLCY_001872"
     CDS             261..1634
                     /gene="aroP"
                     /locus_tag="ACHLCY_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP0369138.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1677..2531)
                     /gene="ampE"
                     /locus_tag="ACHLCY_001873"
     CDS             complement(1677..2531)
                     /gene="ampE"
                     /locus_tag="ACHLCY_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP0369139.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQMPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2528..3079)
                     /gene="ampD"
                     /locus_tag="ACHLCY_001874"
     CDS             complement(2528..3079)
                     /gene="ampD"
                     /locus_tag="ACHLCY_001874"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP0369140.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3167..4060
                     /gene="nadC"
                     /locus_tag="ACHLCY_001875"
     CDS             3167..4060
                     /gene="nadC"
                     /locus_tag="ACHLCY_001875"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP0369141.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4263..4703
                     /gene="ppdD"
                     /locus_tag="ACHLCY_001876"
     CDS             4263..4703
                     /gene="ppdD"
                     /locus_tag="ACHLCY_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP0369142.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4713..6098
                     /gene="gspE"
                     /locus_tag="ACHLCY_001877"
     CDS             4713..6098
                     /gene="gspE"
                     /locus_tag="ACHLCY_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP0369143.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6088..7290
                     /gene="hofC"
                     /locus_tag="ACHLCY_001878"
     CDS             6088..7290
                     /gene="hofC"
                     /locus_tag="ACHLCY_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP0369144.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7325..8368)
                     /gene="guaC"
                     /locus_tag="ACHLCY_001879"
     CDS             complement(7325..8368)
                     /gene="guaC"
                     /locus_tag="ACHLCY_001879"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP0369145.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8524..8568)
                     /locus_tag="ACHLCY_001880"
     CDS             complement(8524..8568)
                     /locus_tag="ACHLCY_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369146.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8593..9213
                     /gene="coaE"
                     /locus_tag="ACHLCY_001881"
     CDS             8593..9213
                     /gene="coaE"
                     /locus_tag="ACHLCY_001881"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP0369147.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9213..9956
                     /gene="zapD"
                     /locus_tag="ACHLCY_001882"
     CDS             9213..9956
                     /gene="zapD"
                     /locus_tag="ACHLCY_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP0369148.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9966..10163
                     /gene="yacG"
                     /locus_tag="ACHLCY_001883"
     CDS             9966..10163
                     /gene="yacG"
                     /locus_tag="ACHLCY_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP0369149.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10264..10653)
                     /gene="mutT"
                     /locus_tag="ACHLCY_001884"
     CDS             complement(10264..10653)
                     /gene="mutT"
                     /locus_tag="ACHLCY_001884"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP0369150.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10713..13418)
                     /gene="secA"
                     /locus_tag="ACHLCY_001885"
     CDS             complement(10713..13418)
                     /gene="secA"
                     /locus_tag="ACHLCY_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP0369151.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAAPAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13480..13992)
                     /gene="secM"
                     /locus_tag="ACHLCY_001886"
     CDS             complement(13480..13992)
                     /gene="secM"
                     /locus_tag="ACHLCY_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP0369152.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14223..15140)
                     /gene="lpxC"
                     /locus_tag="ACHLCY_001887"
     CDS             complement(14223..15140)
                     /gene="lpxC"
                     /locus_tag="ACHLCY_001887"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP0369153.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15241..16392)
                     /gene="ftsZ"
                     /locus_tag="ACHLCY_001888"
     CDS             complement(15241..16392)
                     /gene="ftsZ"
                     /locus_tag="ACHLCY_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP0369154.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16453..17715)
                     /gene="ftsA"
                     /locus_tag="ACHLCY_001889"
     CDS             complement(16453..17715)
                     /gene="ftsA"
                     /locus_tag="ACHLCY_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP0369155.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17712..18542)
                     /gene="ftsQ"
                     /locus_tag="ACHLCY_001890"
     CDS             complement(17712..18542)
                     /gene="ftsQ"
                     /locus_tag="ACHLCY_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP0369156.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18544..19464)
                     /gene="ddlB"
                     /locus_tag="ACHLCY_001891"
     CDS             complement(18544..19464)
                     /gene="ddlB"
                     /locus_tag="ACHLCY_001891"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0369157.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVTPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19457..20932)
                     /gene="murC"
                     /locus_tag="ACHLCY_001892"
     CDS             complement(19457..20932)
                     /gene="murC"
                     /locus_tag="ACHLCY_001892"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP0369158.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20986..22053)
                     /gene="murG"
                     /locus_tag="ACHLCY_001893"
     CDS             complement(20986..22053)
                     /gene="murG"
                     /locus_tag="ACHLCY_001893"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP0369159.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22050..23294)
                     /gene="ftsW"
                     /locus_tag="ACHLCY_001894"
     CDS             complement(22050..23294)
                     /gene="ftsW"
                     /locus_tag="ACHLCY_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP0369160.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23294..24610)
                     /gene="murD"
                     /locus_tag="ACHLCY_001895"
     CDS             complement(23294..24610)
                     /gene="murD"
                     /locus_tag="ACHLCY_001895"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP0369161.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24613..25695)
                     /gene="mraY"
                     /locus_tag="ACHLCY_001896"
     CDS             complement(24613..25695)
                     /gene="mraY"
                     /locus_tag="ACHLCY_001896"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP0369162.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25689..27047)
                     /gene="murF"
                     /locus_tag="ACHLCY_001897"
     CDS             complement(25689..27047)
                     /gene="murF"
                     /locus_tag="ACHLCY_001897"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP0369163.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27044..28531)
                     /gene="murE"
                     /locus_tag="ACHLCY_001898"
     CDS             complement(27044..28531)
                     /gene="murE"
                     /locus_tag="ACHLCY_001898"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP0369164.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28518..30284)
                     /gene="ftsI"
                     /locus_tag="ACHLCY_001899"
     CDS             complement(28518..30284)
                     /gene="ftsI"
                     /locus_tag="ACHLCY_001899"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP0369165.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30300..30665)
                     /gene="ftsL"
                     /locus_tag="ACHLCY_001900"
     CDS             complement(30300..30665)
                     /gene="ftsL"
                     /locus_tag="ACHLCY_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP0369166.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPAVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30662..31603)
                     /gene="rsmH"
                     /locus_tag="ACHLCY_001901"
     CDS             complement(30662..31603)
                     /gene="rsmH"
                     /locus_tag="ACHLCY_001901"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP0369167.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31605..32063)
                     /gene="mraZ"
                     /locus_tag="ACHLCY_001902"
     CDS             complement(31605..32063)
                     /gene="mraZ"
                     /locus_tag="ACHLCY_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP0369168.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32664..33668)
                     /gene="cra"
                     /locus_tag="ACHLCY_001903"
     CDS             complement(32664..33668)
                     /gene="cra"
                     /locus_tag="ACHLCY_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP0369169.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33750..33836)
                     /locus_tag="ACHLCY_001904"
     CDS             complement(33750..33836)
                     /locus_tag="ACHLCY_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP0369170.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33848..34339)
                     /gene="ilvN"
                     /locus_tag="ACHLCY_001905"
     CDS             complement(33848..34339)
                     /gene="ilvN"
                     /locus_tag="ACHLCY_001905"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0369171.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34342..36066)
                     /gene="ilvI"
                     /locus_tag="ACHLCY_001906"
     CDS             complement(34342..36066)
                     /gene="ilvI"
                     /locus_tag="ACHLCY_001906"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP0369172.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36185..36229
                     /locus_tag="ACHLCY_001907"
     CDS             36185..36229
                     /locus_tag="ACHLCY_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369173.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36384..37328)
                     /gene="leuO"
                     /locus_tag="ACHLCY_001908"
     CDS             complement(36384..37328)
                     /gene="leuO"
                     /locus_tag="ACHLCY_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP0369174.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9028 a        10453 c         9164 g         8841 t
ORIGIN      
        1 aaacgaattt aaattcattc tacatattga gaggggttga ggctgagctt tacaaacggt
       61 ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc tgcctcatac
      121 cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt gatgtaaaca
      181 aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag atcaaaaaaa
      241 caaccaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag ctaaagcgcg
      301 gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg accgggttat
      361 tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg ggttacgcca
      421 ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg gtcgaagaac
      481 ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt gccggtttcg
      541 cctctggctg gaactactgg gtactgtacg ttttagttgc catggctgag ctgactgccg
      601 tgggtaaata cattcagttc tggtatccgg aaatccccac ctgggtttct gccgccgtat
      661 tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc gagatggagt
      721 tctggtttgc cattatcaaa gttatcgcgg tggtagcgat gatcatcttc ggcggctggc
      781 tgctattcag tggcaacggc ggcccgcagg cgaccgttag caacctgtgg gatcagggtg
      841 gtttcctgcc gcacggcttc accgggctgg tgatgatgat ggcgattatc atgttctcgt
      901 tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg gagcaaagta
      961 taccgaaagc aactaaccag gttatctacc gcatcctgat tttctatatt ggttcgttag
     1021 ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt ccgtttgtgc
     1081 tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc gtggtactga
     1141 ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg ctgtttggtc
     1201 tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgt ggtgtaccag
     1261 taaataccat tctggtgtct gcactggtaa cggcgttgtg cgtactgatt aactaccttg
     1321 ccccagagtc cgctttcgga ctgttaatgg cgctggtggt atctgcactg gtaatcaact
     1381 gggcgatgat tagcctggcg catatgaaat tccgtcgcgc caagcaggaa caaggcgtgg
     1441 taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg ctgtttatgg
     1501 cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta tacctgatcc
     1561 cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc gccaaagccg
     1621 taaaagcgca ttaatctctc tacgccctca cccgtacagg gtgagggcaa taatctttac
     1681 accaacgccc cgtaaatcgt cagtagtgca atcaccacca cgaccacgaa cgaggttttc
     1741 ttcgccattg aaaccgctgc cctcggcgtc tccaccttat cgacatgcgg ttcacgcgcc
     1801 agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa atcacccagc
     1861 gaagcaaacc aggccggtaa cgctttctca ccatgaccga tcaaggcata taccacaccc
     1921 gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc ggactgtaaa
     1981 cgatgatgcg gcatctgata tcgcgccagc cagtattgcc atgcacgcaa aaacgcatac
     2041 cccatcagcg taacgggtcc ccaggttccc cccacaatca gccagaacag cggtgcaaga
     2101 taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata ctcacgttcg
     2161 tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt ggcacgggca
     2221 tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg aactttacct
     2281 gcgccaatac acagcaaacc aatcagcagc cacaccagta gcgtgggaac gttgaacaat
     2341 actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat ggtcatgcct
     2401 aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag acgatgatca
     2461 agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag taaggttgta
     2521 aatagcgtca tgttgtctcc ttgctgacca gcacacgaaa ccgtgcccaa tcaaatgcag
     2581 gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg tttttagcga
     2641 tatccggata gcaatcaatc agtgcccgcg taaccgccgc aagctgttga tactgcgcat
     2701 cggtatacgc cagcgtatcg gtgccttcaa gctcaatccc aatagaaaaa tcattgcagc
     2761 gttcgcgccc ctgatactga gagactcccg catgccatgc acgtttatcg aaaggaacat
     2821 actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc aaatgggcga
     2881 tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat gcgtcgatcc
     2941 acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc agggtgggtg
     3001 tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg ccaaccagcc
     3061 acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc cggttcagag
     3121 tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc cgcctcgccg
     3181 ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg atatccccgg
     3241 cgcggtagcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca acaatgatat
     3301 tacggcaaaa cttttaccgg aaaattctcg ctctcatgcc acggtgatca cccgcgagaa
     3361 tggcgtcttt tgcggcaaac gctgggttga agaggtgttt attcaactgg caggcgacga
     3421 tgtcaccata atctggcatg tggatgacgg cgatgtcatc aatgccaatc aatccttgtt
     3481 cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc ttaattttgt
     3541 gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattgc tggaaggcac
     3601 caacacgcag ttgttggata cgcgcaaaac cttacccggc ctgcgttcag ctctgaaata
     3661 cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg ccttcctgat
     3721 caaagaaaac catattattg cctccggctc agtgcgccag gcggtcgaaa aagcgtcctg
     3781 gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac ttgatgaagc
     3841 cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac agatgcgcga
     3901 agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg tcactgacaa
     3961 aacactgcgt gaatttgccg aaacgggcgt ggactttatc tccgtcggtg cgctaactaa
     4021 acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa tagttgacag atttatccgc
     4081 tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct cgcaaatttc
     4141 ttgtctttcg ctgcaacgaa ataaaaaacc tcggaatctg ctttcccgtt tcgttttttg
     4201 cccctcgcct gtgctgatgg cgtttgccaa agtagcgcca actaaatcaa ggagcgaaac
     4261 agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt ggcatcattg
     4321 ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa gccgcactca
     4381 ccgacatgct acaaaccttt gtgccttacc gtaccgccgt agagttgtgc gcgctggaac
     4441 atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct accaccaccc
     4501 gctatgtttc agccatgagt gtggcaaagg gcgtggtgtc gctgaccggg caagaaagtc
     4561 tcaatgggct aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc gtcaccggct
     4621 ggacgcgcaa ctgcaatatt caaagtgaca gcgcattgca tcaagcctgc gaagatgtct
     4681 tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc actgccctgt
     4741 gtctgcgtta tcatggagtc ttgctggatg ccagcgaaga ggtggttcat gttgcggtag
     4801 tcgatgcacc ttcgcatgag ctactggacg cattgcattt cgctaccacc aaacgtattg
     4861 agatcacctg ctggacgcgc caacaaatgg aaggtcacgc cagtcgcaca caacagacat
     4921 tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctaact cgaacgttac
     4981 aatctgcgct ggaacaacgc gcgtctgata ttcatatcga accagcggac aatgcctacc
     5041 gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca ccggatgccg
     5101 gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg gaacatcgcc
     5161 tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca tttcgtattg
     5221 cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag caggtgagcc
     5281 aggcactgga tgtcaacacg cttggaatgc agccgttaca actggcggac tttgctcatg
     5341 ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc ggcaaaacgg
     5401 tcacgcttta tagtgccctg caaacgctga ataccgctga cattaatatt tgtagcgtcg
     5461 aagatccggt tgagatccct atagccggac taaaccagac gcaaatccat ccgcgtgccg
     5521 gactcacctt tcagggcgtg ttgcgtgcgt tattgcgcca ggatcctgac gtcatcatga
     5581 tcggagagat ccgcgatggc gaaacagcag agatcgctat taaagcggcg caaactggtc
     5641 acctggtgtt gtctacccta cacactaatt ccacctgcga aacgctggta cgtttacagc
     5701 aaatgggggt cgcccgctgg atgctatcat cggcgcttac gctggtaata gcccagcgtc
     5761 tggtacgcaa actttgccca cattgtcgcc ggcagcaagg ggagcccatc cacattccag
     5821 acaatgtatg gccatcgccg ctgccccact ggcaggcacc cggttgtgta cattgctacc
     5881 acggttttta tggtcgtacg gccttatttg aagttctgcc cataacgccg gtcattcgtc
     5941 agcttatttc cgctaatacc gacgttgaat cgctggaaac gcacgcacga caggcgggta
     6001 tgcgtacgct ttttgaaaac ggctgcctgg ccgtggagca aggcttaacc acctttgaag
     6061 agttaatccg cgtactgggg atgccgcatg gcgagtaagc aactctggcg ctggcatggc
     6121 ataaccggcg acggcaatgc gcaagatggg atgctatggg cagagagccg tgctttgctg
     6181 ctcatggcac tacagcaaca gatggttacc ccacttagcc tgaagcgaat cgccatcaat
     6241 tctgcgcagt ggcgaggaga taaaagcgcg gaagtcattc atcaactggc gacgctactc
     6301 aaagccgggt taacgctttc tgaagggctg gcactgctgg cggaacagca tcccagtaag
     6361 caatggcaag cgttgctgca atcgctggcg cacgatctcg aacagggcat tgctttttcc
     6421 aatgccttat taccctggtc agaggtattt ccgccactct atcaggcgat gatccgcacg
     6481 ggtgaactga ccggtaagct ggatgaatgc tgctttgaac tggcgcgtca gcaaaaagcc
     6541 cagcgtcagt tgaccgacaa agtgaaatca gcgttacgtt atcccatcat cattttagcg
     6601 atggcaatca tggtggttgt ggcaatgctg cattttgttc tgccggagtt tgccgctatc
     6661 tataagacct tcaacacccc actaccggca ctaacgcagg ggatcatgac gctggcagac
     6721 tttagtggcg aatggagctg gctgctggtg ttgttcggct ttctgctggc gatagccaat
     6781 aagttgctga tgcgccgacc gacctggctt atagtgcggc agaaattgct gttacgcatc
     6841 ccgattatgg gttcactgat gcggggacaa aaactcacgc agatttttac gattctggcg
     6901 ctgacacaaa gtgcaggcat tacttttttg cagggcgtag agagcgtcag agaaacaatg
     6961 cgctgcccgt actgggtgca acttctgaca caaatccagc acgatatcag taacggtcat
     7021 cccatctggc tggcgctaaa aaataccggg gagtttagcc cgctctgttt gcaattagtg
     7081 agaacaggag aggcatccgg ctcgctggac ctcatgttag acaacctcgc ccatcatcat
     7141 cgggataaca caatggcgct ggcggataac ctcgcagcct tactggaacc ggcgttgctg
     7201 atcataacgg gaggaattat cggtacgctg gtggtggcga tgtatctgcc aattttccat
     7261 ttaggcgatg cgatgagtgg gatgggataa cgctggcgtg ttgctccaca ccagcgttgg
     7321 aagattacag gttgttgaag atgcggtttt cttgttcctg cacacggata aatgtggtgc
     7381 gcttggtcag ttctttcagg cgtgaagccc caacgtatgt acaagctgaa cgcaggccgc
     7441 ccaaaatatc acgcgcggta ttttcaaccg ggcctcgcag cggcagctta acggttttac
     7501 cttctgctgc gcgatattcc gcaacgccgc caacgtgacg tttcatcgca gactcggagc
     7561 tcatgccgta gaacagcata aatttctcgc cgttctcctc aacgatgcga ccgccgctct
     7621 cttcgtggcc cgccagcatg ccgccaagca tgacgaaatc ggcaccgccg ccaaaggctt
     7681 ttgcgacatc gcccggcgtg gtgcagccac cgtcgctgac gatcattccg cccagaccgt
     7741 gcgccgcatc ggcacattcg attaccgcag aaagctgcgg ataaccgacg cctgttttga
     7801 cgcgagttgt acaaacagaa cctgggccaa tgccaacttt aacgatatca gcaccggaga
     7861 ggataagctc ctcacacatt tcaccagtca ctacgttgcc agcacaaatg gttttggtcg
     7921 gccacgcttc acgcgctttc gcaacgaact gcacgaagtg ttcggaataa ccattcgcca
     7981 catcaataca aacgaagttt aatgccgggt tcaggtcgag aatctgttta gttttttcga
     8041 aatccgcatc agacgtaccg gtagaaacca tcacatgttt cagcacatca gcggaagaat
     8101 tgttgataaa cgcttgccac tcttcgacag aatagtgttt atgcacagca gtcaaaatat
     8161 caaaagaagc cagcgcagag gccatagaaa atgtacctac ggtgtccata tttgcggcga
     8221 taattggcac gccggaccag ctctgacctg aatgtttgaa ggtgaattga cgttccagtt
     8281 caacatcgga acggctttta agagtggagc gtttagggcg gatgagaacg tctttaaaac
     8341 ctaacttcag atcttcttca atacgcatgt gcggattcct ggggttaatg gctataaggg
     8401 taagagcgca actccagtga cgctatcata cgcactaatc aatgcggcgc aagactgcga
     8461 aattgtcttt ttttacgcta caatcccata aatttacctg gtaaatagtg agcgaacgtt
     8521 tcatcaattt cgctttcgtg cactcatttt taacgttttg ataatcatga tgaattcccg
     8581 ggaatataaa ttatgaggta tatagttgcc ttaacgggag gcattggcag tggcaagagt
     8641 accgttgcca atgcgtttgc tgatctcgga attaacgtca ttgatgccga tattattgcg
     8701 cgtcaggtgg ttgaaccagg tgcacctgcg ctacatgcca ttgctgatca ctttggcgct
     8761 aacatgattg ctgctgatgg aacattgcag cgccgggcct tgcgcgagcg gatcttcgcc
     8821 aacccagaag agaaaaactg gcttaacgcc ctgctgcatc cgctgattca gcaagagacg
     8881 caacaccaga tccagcaagc tacttccccc tatgtactgt gggttgtgcc attgctggta
     8941 gaaaactcac tgtataaaaa agcgaatcga gtgctggtgg tggatgtcag cccagaaacg
     9001 caacttaagc gcaccatgca gcgcgatgat gtaactcgcg agcatgtcga acaaatcctt
     9061 gctgctcagg caacgcgcga agcccgcctt gccgtggcag atgacgtcat tgataataac
     9121 ggcgcaccgg atgctatcgc atcggatgtt gcccgcctgc acgcacacta tttgcagctt
     9181 gcgtcgcagt ttgtctcaca ggaaaaaccg taatgcagac ccaggtcctt tttgaacatc
     9241 cactaaatga aaaaatgcgt acatggctgc gcattgagtt tttgattcag caactcaccg
     9301 ttaatttacc catcgttgac cacgctggcg cgctgcattt cttccgtaat gtcagtgaat
     9361 tactggatgt tttcgagcgc ggcgaagtcc gcactgagct gttgaaagaa cttgaccggc
     9421 agcaacgtaa actccagacc tggattggcg tgcctggcgt ggaccagagc cgtattgaag
     9481 cattaattca gcagttaaaa gcggcgggga gcgtattaat ttccgcgccg cgtatcgggc
     9541 aatttctgcg tgaagatcgt ttgattgctc tggtgcgtca gcgactgagc atcccaggcg
     9601 gctgttgcag ctttgattta cctacattgc acatttggct gcatctaccc caggcgcagc
     9661 gcgacagcca ggtagaaacc tggattgcca gcctgaaccc gctcacccag gcacttacca
     9721 tggtgctgga tttaattcgc cagtcggctc ccttccgtaa acaaaccagc ctgaatggtt
     9781 tttatcagga taacggtggc gatgccgact tgctgcgcct gaatctgtcg ctcgattcac
     9841 agctttatcc gcaaatttcc ggacataaga gccgttttgc cattcgtttt atgccgctgg
     9901 acactgaaaa cggacaggta ccggaacgtc tggatttcga actggcctgt tgctaaggag
     9961 tgaaaatgtc agaaactatt acggtgaatt gcccaacctg cgggaaaacg gtggtgtggg
    10021 gtgaaatcag cccgtttcgg ccattttgct ccaaacgttg tcagctgatc gacctcggag
    10081 aatgggctgc tgaagaaaaa cgaatcccca gcagtggcga tctttccgaa agcgatgact
    10141 ggagcgaaga gccaaagcag tgacatttgt ctgatgccgc acgtagatac tgataagacg
    10201 cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa cgccttatcc
    10261 gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc
    10321 attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc acggttcccc
    10381 ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatattc
    10441 cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct gaagttcacg
    10501 caccaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa actccagttt
    10561 attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt tctcgttgcg
    10621 aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa aaaggcgcag
    10681 aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg gcactgcttg
    10741 tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ctcaccggtt
    10801 tgcgccgccg gtgcagctgc ggctgcagag tcgtcatcct gatggctaag ctgctgcatt
    10861 tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc
    10921 atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga ctccagcatc
    10981 gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag
    11041 ccacgcaggt ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg
    11101 gagtcaagcg tttgcagcat gacgcctttc tcgaagtgac gcatcatctc agcaccaacc
    11161 acttcttctt tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc
    11221 tcttcatgca gttctggttc tttatccagc cactcggcaa ttggtaaatc gaggtcgaaa
    11281 tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag cgactgtggc
    11341 ggaatgtagg catcaatggt ggctttgaac acatcttcac gaatgctgtt gatggtttcg
    11401 ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg
    11461 ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact
    11521 ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc
    11581 ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcagc
    11641 gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga
    11701 ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg
    11761 ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt
    11821 tgctctacgg tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca
    11881 atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg ataacctgcc
    11941 tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt gtgcttaata
    12001 ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat agtacccacc
    12061 agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt
    12121 tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc
    12181 ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc ggtatcagca
    12241 gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa ggtgatcgaa
    12301 gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc
    12361 agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata
    12421 acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg agcgcgcagc
    12481 gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca
    12541 tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg ttcggtcagg
    12601 ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa ggtttcggag
    12661 tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc
    12721 gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc gatcaggatg
    12781 gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc ttcagggctg
    12841 aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg
    12901 tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt gataccgaca
    12961 gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag
    13021 tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta agcaggcagc
    13081 gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga
    13141 accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc
    13201 tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga
    13261 cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt ttccatctcc
    13321 ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta
    13381 cgactaccga aaactttagt taacaatttg attagcataa taaaatctca aacgccccgc
    13441 gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc gttgagggcc
    13501 agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt
    13561 aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcagcag
    13621 cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc
    13681 aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg tgcgaatggc
    13741 atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt tcgcttccag
    13801 caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt
    13861 tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcaac
    13921 catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag
    13981 tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg cacaaatctt
    14041 atcattaaag cctgcagtcc gcagcacgaa tgaagataag ttgtgcggaa aagtgcttaa
    14101 gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa tttaaaaaaa aacgactggt
    14161 tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt
    14221 cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc gtcgtcctgg
    14281 aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag tttgttattc
    14341 agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac
    14401 aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg
    14461 tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg
    14521 cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg
    14581 ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa
    14641 tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta
    14701 aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg aacaaatttt
    14761 ttggcgcagt tcaactcgtc gataccggcg tcaagcagca agtatacaaa cggagcggcg
    14821 ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc
    14881 aagcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc atgctcgttg
    14941 accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc
    15001 ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac
    15061 gtcagggtga ctttcttgcc ggtatgtaag ccgacacccg tcgcctgaac gatacgttta
    15121 agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac
    15181 tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc
    15241 ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt
    15301 ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc
    15361 catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag
    15421 agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta cgcgcagctc
    15481 gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa
    15541 tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgccgt
    15601 gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca gcggagaaga
    15661 gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat
    15721 cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa
    15781 acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta catcgttcgc
    15841 tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt
    15901 cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa
    15961 tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat
    16021 acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc
    16081 catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca atgcatcgcg
    16141 atcctcatca gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt
    16201 gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc
    16261 ggtatttacc gcgaagaatt caacaccttc aatgcgctcg cgcaccatgt gttcaacagc
    16321 attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat
    16381 tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg
    16441 gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc
    16501 aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc
    16561 atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc
    16621 ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc
    16681 tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg
    16741 tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccaggtt
    16801 gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg
    16861 cggcggacga ccacctacgc tcggcacttc cacgctctca tcttttccaa cgatggaacc
    16921 cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg tgccaaaggc
    16981 gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag tgtggcgcaa
    17041 tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga cgacgcagac
    17101 acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat
    17161 cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc
    17221 gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa gtcctaccgg
    17281 attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg
    17341 atgctcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg
    17401 cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt
    17461 accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc
    17521 aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat
    17581 accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc
    17641 ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc
    17701 cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct
    17761 ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta
    17821 atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc tacaaagcga
    17881 gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc
    17941 aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc
    18001 agcatctgcc ccatttcgcg ataaccctgc aacacttcat tggcgctgcc ttccgggcca
    18061 tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa ggtgtttcct
    18121 tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga
    18181 atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt
    18241 tgttctattt gcgtctggat gatgttgaca tcttgggtca taaaggtacc cggctcaccc
    18301 aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac
    18361 accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg
    18421 ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcgttcca
    18481 ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc agcctgcgac
    18541 atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg
    18601 acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag
    18661 cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc ctttgcaacc
    18721 taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg acgcttccag
    18781 acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa
    18841 ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt
    18901 gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa
    18961 tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca cactggaacc
    19021 ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg ctaactgctt
    19081 atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg tgaccggtaa
    19141 accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg cagatgccat
    19201 cactccgctg ccggtataag gcaagcccat cagctcgagc atgccctgca gcgtaccatc
    19261 ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg acttcagttg
    19321 cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt cacgcagccc
    19381 ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag tcccgcccaa
    19441 caggaccgcg attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga
    19501 tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg agaatcaggt
    19561 cgttaccggt taataccggt gccagcatct cggctacctg cgcagggtcc ggcaccagaa
    19621 tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg
    19681 gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca
    19741 gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc ggctgaaaca
    19801 gcattaccag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg gcgtcaactt
    19861 ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta ccattcactg
    19921 gctccagcgg gaattcaccg aggaaatcaa aacggcgccc agtcccctgg aagctctcca
    19981 gcgcacgtaa aatagcctcg tcatcaattc cctcttccgt agcaaccgca accgcagctg
    20041 cggcgttcag cgcgttatga cgacctggcg cattcagggt gacgcgcatc ggctctttgt
    20101 cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca
    20161 cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccactcgc ggtaacaatt
    20221 cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca
    20281 gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt
    20341 cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac
    20401 tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg
    20461 cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg
    20521 aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg ccatgacgaa
    20581 aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc gcttcatgag
    20641 ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc acgctggcat
    20701 cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc attaactgct
    20761 gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa
    20821 cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc
    20881 gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg
    20941 ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc
    21001 ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg
    21061 ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc
    21121 cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc
    21181 attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc
    21241 cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat ccgcccacgc
    21301 atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt gccccgcttc
    21361 ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc agatagtgac
    21421 tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg
    21481 ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg
    21541 caacggcagc gccagtacat cggtacgcac cgggttaccc accacttccg cattagggaa
    21601 agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt tattggttaa
    21661 gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag
    21721 accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt tgtacgcttt
    21781 cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag ctatcagtgc
    21841 ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt
    21901 tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg
    21961 agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg ttccgcctgc
    22021 catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct
    22081 ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc
    22141 agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc
    22201 cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc aatagaacag
    22261 gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat cgacatcgcg
    22321 cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct
    22381 tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt
    22441 accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat cagcgattgc
    22501 gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg
    22561 cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc cataccgata
    22621 atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca
    22681 aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga
    22741 atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc
    22801 accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc aggctggata
    22861 cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc cactaccagc
    22921 acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac
    22981 tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca
    23041 tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc
    23101 gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag taaggtgcga
    23161 tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccttttagc
    23221 gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt caggcgaggg
    23281 agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt
    23341 caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc atatcgcccg
    23401 gctgaacacg cggagccagc aagcgcatcg cctgttccat agtttcggtt tgttctgcca
    23461 cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac aaacgcacgt
    23521 tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca tcgccgccca
    23581 acaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc gtactgccga
    23641 cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc accacttcaa
    23701 agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg cgcggcaaac
    23761 cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc tgcccggaaa
    23821 gtttcatctc tttcacgttc agcactttct cgcctttcac tcgcagccag gtttctccct
    23881 gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag cgttcatcag
    23941 caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact ttcgcatttt
    24001 cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag cggtccatat
    24061 gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa gtggtttcca
    24121 gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc agcatcaacg
    24181 ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac
    24241 ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca atcggtgctt
    24301 gtgcttcgcg gcagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag
    24361 cgcttaagga tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca
    24421 tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt aatttatcca
    24481 ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa
    24541 agtccacgca ggaaagccca gtgaggccca ggccgataat gacgacattt ttaccctgat
    24601 aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca gcatcagcga
    24661 aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg
    24721 atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag
    24781 gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg
    24841 acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac ctacatcgcc
    24901 cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg ccccgactat
    24961 cgccgtgcag acaatgacca gttccccggc gtgtcgcaga tacggtatgt gtaaatagct
    25021 ggcaaagttc atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac
    25081 ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt tgccagtacc
    25141 cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca tcacatcttt
    25201 aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagggcgaa
    25261 ggcgataccc agcgcaatga ccgacatcca gaaatacttc caacgagcga tcaacccttt
    25321 ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac cgtaacctac
    25381 cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca gcagtacgga
    25441 gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc gcttgctgaa
    25501 gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc
    25561 aatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc
    25621 gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc
    25681 cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcggca
    25741 ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga
    25801 cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc ggtgctgata
    25861 gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt tgccgcctcg
    25921 cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc
    25981 agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat tgaaccgaca
    26041 ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat ggggaacaga
    26101 cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc
    26161 acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccggcaac
    26221 ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt accgtgcgag
    26281 gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga gaaacgccac
    26341 actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc ggcgttcata
    26401 atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc
    26461 gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgcagcttcc
    26521 gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc aagttcaata
    26581 actgcgtaat cgtattccgg cgttaagcgc aacagcgtca tcggtacacc gatgtcgttg
    26641 ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc cgccgtcatc
    26701 tctttaacgg aggttttgcc ggaggacccc gtcagagcaa ccacgcgcgc cggaacttgc
    26761 tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc
    26821 ggcaggtcga tgtccagcgg acggctaacc agtagtgcgc ctgcgccgcc agctttcgcc
    26881 tggtcggcaa aatcgtgggc atcaaaacgt tcgcctttca gggcaacaaa caggcagccc
    26941 ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc accttgcagt
    27001 tcaccgttga gaatatcggt aagttggcta agggttacgc taatcatgca atcaccccca
    27061 gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt
    27121 aatcctcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc tgcataacgg
    27181 cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca tctaacattc
    27241 ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta tcgtccgtca
    27301 ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc ttacctttat
    27361 cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc aggcgcgccg
    27421 cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt
    27481 tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt
    27541 ttaacagatc agccagcggg tagccaagtg ccagcagtgt cgccagcgcg agcagcaggt
    27601 tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc caacttgaac
    27661 taaagcgaat ggtcgcgccg ctgtcgtgat agttcacttc ggtcgctttc aaccagcgtc
    27721 cgtgacagtt cggattgata tgatcttcca ttgataccgc aaccgcgtcc ggcagttttg
    27781 ccagccagcg gcggcccact tcatcgtcgg cgttaacaat cgcctgacca caatgatgtt
    27841 cagaataaag cagccatttc gcggcttcgt agtgttccat atcaccatga taatcaaggt
    27901 gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgct
    27961 gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctga tccaccagcc
    28021 ccgccagctc atgctgaaca tcgactgccg aaccggtggt attttctgtc gggatcactt
    28081 tccccagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc
    28141 tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt acgcctacga
    28201 gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt
    28261 tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg
    28321 cctcaccttt cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat
    28381 atcgacgccc gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca
    28441 cacggctgtc gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag
    28501 caagaaggtc gcgcaaatta cgatctgcca cctgtcccct cgccttgatt aatcacaaat
    28561 tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg
    28621 cccatgatgg caccaaagac cggcgctgaa acggcgccgc cgtagtattt acccgcctgc
    28681 ggatcgttga taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg
    28741 gtataagcaa tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg
    28801 gttttaatgg cgatacgata gcctttaatc gccgccttca cgccgccgcc gcctggtagc
    28861 gccacgcttt ccatcatatg caccacagtg cggacaatgg attccgggaa gacacgttca
    28921 ccgggaaccg gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg
    28981 atagttgcgt agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag
    29041 aaggtggccc tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct
    29101 ccgaccaacc ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac
    29161 gctgaggacg gcatcgctaa cgccagcttg gaaacaccga cgttactcga cttctgtaat
    29221 accccggtca gggttaattc gctgtagcgt gccacgtctt tgatttcgtg gccgttaatt
    29281 cgataaggaa tggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc
    29341 attaccacca tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta
    29401 cgcatcgcct ctttcggcgt gccgctcaga ttgttagggt tgtatgacgg gctgttagcc
    29461 atcgccagca cttcaccggt gttgacatcc accagcacgg cgctaccaga ttcagccttg
    29521 ttaaaggcca ccgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata
    29581 ctcagcgcca ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga
    29641 ccatagcggt ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa
    29701 ctcttctcaa cgccctcaat cccttgacta tcgacgttag taaagccgat gaggtgagca
    29761 gtcacttcgc cggacggata gtaacggcga gactcttcac gcagatgaat ccccggcagt
    29821 ttcagttttt tgatgtagtc cgccatgtca gggttcacct gacgcgccag ataaataaag
    29881 cgccctttcg ggttggcgtt aatgcgggct gaaagctgat ccagcggaat attgagcgcg
    29941 ttagccagcg ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttc
    30001 gggtcagccc aaatcgcttt taccggcacg ctcaccgcta acgggcgacc agaacggtca
    30061 gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct
    30121 ttcaccagca tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc
    30181 agcgccagga gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt
    30241 tcttcctgac gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct
    30301 tatttttgca ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc
    30361 gtggcgatcc tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg
    30421 cgccattcaa tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt
    30481 aaacgggtat ggtgcgccgt ggttaccaca gtcaccgccg tcaaaataat gcaaatgaac
    30541 aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac cgataacagc aggcaatgca
    30601 tggcgctcgt ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg
    30661 atcatgcatt cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag
    30721 ccacctcttc ttcgcccggc attaacttgc ctagtgctcg cagctgacgg ccacccagtt
    30781 ttttgagctg ctcttcagtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt
    30841 cacgcataaa acgtttcaca atacggtctt ccagcgagtg gaagctgatg atcgaaagcc
    30901 gcccacccgg ggccagcacg ttgagcgagc tttttagcgc ctgctctatc tcctccagtt
    30961 cactgtttac ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat
    31021 ctttcaccgg cgttgcagca gccacgactt ccgccagttc tttggtgcgg gtcatcggct
    31081 gttcgcggtt acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat
    31141 aggttttcaa tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg
    31201 actgcccacg ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc
    31261 cgcgctcagc atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga
    31321 tcttgccgat aagatcgcgc tcggcaacgt attcgcccag cgcggagaaa ggtccgtgga
    31381 tgatggagaa gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat
    31441 cgatcgccag caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac
    31501 cgcgaccaaa agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg
    31561 cttcatccag cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc
    31621 tgcagtcgct ccgataagtc tccggtagcc aactgctctg cgtcgatatc ttccttgacc
    31681 tgttgatgcc aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc
    31741 acttcttttg tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg
    31801 ccatccatct gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc
    31861 gggttcatgc tcgacagacg cgataatttt tgctcgataa tttcccattc aggtaggggg
    31921 taaagcagca ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcgttc
    31981 tcaagcagct gttcccggta acgggtaggc actgataagc gccctttgct gtcgagattg
    32041 actaacgttg ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat
    32101 ttcccactga aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat
    32161 accgaggtaa aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac
    32221 gtgcgcagca aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcaccg
    32281 ttttaaaatg actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg
    32341 gcattttagg acatatcttc cccggttaac agtccctgtt gcgtctgtgt ggcgcttgat
    32401 gccagcgggt ttgcgcaaaa gcaaagaaaa gtgcttgtta aatcggcaat tcatctctgc
    32461 atgtaacaaa ataatgcaaa acgcgctgtc gatatcactt aattattgtg aatttcagct
    32521 ggtgagttaa ttaacgggcg ggtttaaaag aacttatcaa taatagtcag gaatagtctt
    32581 atttacttta aggatattga tgtccattcc cgtactctac gcgccagagg gaaattcacc
    32641 tggcgcgtat ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta
    32701 cgtttaatgc gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca
    32761 atctccagca cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac
    32821 tgtaagaagt cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt
    32881 ttaccatcgc gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg
    32941 aacagcgcct gcggcatcgg atgcgtttcc agccattttt cgaataactg ggcagccgcc
    33001 tcccgctcat agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca
    33061 gtacggaaac cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa
    33121 agcaccgtct cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga
    33181 tcggcaccaa ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc
    33241 gggaacgggt cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa
    33301 acaataatgg catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct
    33361 ggctgatctt ctgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgctca
    33421 agatagttag cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata
    33481 gaacgtgtgc gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca
    33541 cgcaccacag ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg
    33601 ccgttaataa catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc
    33661 agtttcacaa ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt
    33721 taaattcatt aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt
    33781 cccactgatt tcgcaaaaaa agcccaacgt gacatgttgg gctgcaaatt gcgcactgag
    33841 atcattctca acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa
    33901 cttccacaat tttcgccaca tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg
    33961 tgcctgctaa ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata
    34021 tttccgtatt acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca
    34081 tgatttcccg ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca
    34141 gcttgtgtaa ttgcttttcg atctgctcaa gcactttttc atcgcccacg gtctggatgg
    34201 tcatacgcga taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc
    34261 cacgctggga aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga
    34321 ctgataatat ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat
    34381 tccgcccccg cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac
    34441 aaacaccagg cgattattgc acacctgttc cagcgcctcg ctaagtttgc tttccagctc
    34501 atgcggatga gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc
    34561 gggtagtgat tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg
    34621 tttcaccatc cccagatagc ggttattgag attcaccacc agtaccggca actcgtattg
    34681 caacgcggta gacagttcct ggatgttcat ctgaatactg ccgtcgccag tgacgcaaac
    34741 cacggtttct tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat
    34801 cgtgccgagg ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc
    34861 agcaaacatc tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg
    34921 ccaaagagtc tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag
    34981 gcactgacga gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg
    35041 ttgatgggcg gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc
    35101 ccccacaatc gggatatccg cagtcacggt tttagaaatg gaagtaggat caatatcgat
    35161 atgcagaaca gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa
    35221 tcgtaccccg acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt
    35281 accgtgcatt cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc
    35341 catcaatgag gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg
    35401 gcagcccgcc atgattgccc caccgcctac gtagacaacc ggtttttttg ccgctaccag
    35461 cgtttgcaga gcgcgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg
    35521 catactgacc gactcaggcc agacataggg taatttgttc gccggattaa gaatatcttt
    35581 cggtaaatca acgaccactg gtcctgggcg accacttgcc gccagccaga aagccttttt
    35641 cagcacctgc ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccaccggtcg
    35701 cgaaatcccc accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac
    35761 ctgcccggaa aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat
    35821 cgcattggtc gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg
    35881 cgccagacca tcggccatat gcactgccgc ctgctcatga cgaactaaaa catgatcgat
    35941 accacccacg gtatgcaatg catcgtaaat atcaaggact gcgcctccgg gataaccgaa
    36001 tacttgttta acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat
    36061 ctccatggcc tgcctcactg tttgacggaa aaaatgtgta aaacacatta accgtccgat
    36121 cttgcttaag caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg
    36181 aaaggtgaaa agaaacgata aaatcctcca ttacgaagga ttaaattgat aataaatcgg
    36241 ctgaatccca caacttacag caatttctct atttttttaa ttaatcctaa aacagagagc
    36301 ccacacattc agaataaaat tctacattca ataaaaaata cagaataaac cagacattca
    36361 tgcctgacct attctgcatt cagttagcgt ttgcaaattg agactaattg ctcttccatc
    36421 cactgatggc ctttatcgcg cccggcagct tcatgccagg agagataaca ggttctgcta
    36481 ttttgtttta acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc
    36541 agccaacgcg gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc
    36601 attgccatgc cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga
    36661 ctaaatgacg cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt
    36721 ttcagtaacg ggcccttaat tgttggatga tttttgctgg ctaccagcac catttcatct
    36781 ttaaataatg gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac
    36841 tccgtttcct gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata
    36901 acatgtatat ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg
    36961 tctaacgggc tgcaaacaca aagatgaaat acacgttcac tgctcgcggg ttcaaaacct
    37021 gaaccaggca attcattttg tactagttgc aatgcctgac gaactgagcc aaaaagttga
    37081 aatgcgcgag cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac
    37141 atcaccttca ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga
    37201 gcggcacggg taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga
    37261 tcgaccatgc gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc
    37321 tctggcatac ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga
    37381 aataatcctg aagaatatga tttatcatgc atataaaata agaaaaagca aaatgagtaa
    37441 aattcggaaa acataaagat acgggttctg ataaaaacag aatcca
//
LOCUS       DAZGTR010000034        37155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGTR010000034 DAZGTR010000000
VERSION     DAZGTR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.034"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            42..854
                     /gene="ydhT"
                     /locus_tag="ACHLCY_001909"
     CDS             42..854
                     /gene="ydhT"
                     /locus_tag="ACHLCY_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP0369175.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERAWVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(866..2470)
                     /locus_tag="ACHLCY_001910"
     CDS             complement(866..2470)
                     /locus_tag="ACHLCY_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP0369176.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDIGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(2596..2901)
                     /locus_tag="ACHLCY_001911"
     CDS             complement(2596..2901)
                     /locus_tag="ACHLCY_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP0369177.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(3009..3085)
                     /locus_tag="ACHLCY_001912"
     tRNA            complement(3009..3085)
                     /locus_tag="ACHLCY_001912"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3049..3051),aa:Val,seq:gac)
     gene            complement(3090..3166)
                     /locus_tag="ACHLCY_001913"
     tRNA            complement(3090..3166)
                     /locus_tag="ACHLCY_001913"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3130..3132),aa:Val,seq:gac)
     gene            3474..4730
                     /locus_tag="ACHLCY_001914"
     CDS             3474..4730
                     /locus_tag="ACHLCY_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0369178.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(4771..6144)
                     /gene="mdtK"
                     /locus_tag="ACHLCY_001915"
     CDS             complement(4771..6144)
                     /gene="mdtK"
                     /locus_tag="ACHLCY_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP0369179.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            6359..7000
                     /gene="ribE"
                     /locus_tag="ACHLCY_001916"
     CDS             6359..7000
                     /gene="ribE"
                     /locus_tag="ACHLCY_001916"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP0369180.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(7040..8188)
                     /gene="cfa"
                     /locus_tag="ACHLCY_001917"
     CDS             complement(7040..8188)
                     /gene="cfa"
                     /locus_tag="ACHLCY_001917"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP0369181.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8479..9690)
                     /gene="punC"
                     /locus_tag="ACHLCY_001918"
     CDS             complement(8479..9690)
                     /gene="punC"
                     /locus_tag="ACHLCY_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP0369182.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            9803..10735
                     /gene="punR"
                     /locus_tag="ACHLCY_001919"
     CDS             9803..10735
                     /gene="punR"
                     /locus_tag="ACHLCY_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP0369183.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(10732..11757)
                     /gene="purR"
                     /locus_tag="ACHLCY_001920"
     CDS             complement(10732..11757)
                     /gene="purR"
                     /locus_tag="ACHLCY_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP0369184.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            12056..12145
                     /gene="cydH"
                     /locus_tag="ACHLCY_001921"
                     /gene_synonym="ynhF"
     CDS             12056..12145
                     /gene="cydH"
                     /locus_tag="ACHLCY_001921"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP0369185.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            12311..13480
                     /locus_tag="ACHLCY_001922"
     CDS             12311..13480
                     /locus_tag="ACHLCY_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0369186.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(13626..14207)
                     /gene="sodB"
                     /locus_tag="ACHLCY_001923"
     CDS             complement(13626..14207)
                     /gene="sodB"
                     /locus_tag="ACHLCY_001923"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP0369187.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(14335..15162)
                     /gene="mepH"
                     /locus_tag="ACHLCY_001924"
     CDS             complement(14335..15162)
                     /gene="mepH"
                     /locus_tag="ACHLCY_001924"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP0369188.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            15497..15844
                     /gene="grxD"
                     /locus_tag="ACHLCY_001925"
     CDS             15497..15844
                     /gene="grxD"
                     /locus_tag="ACHLCY_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP0369189.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(15895..20511)
                     /locus_tag="ACHLCY_001926"
     CDS             complement(15895..20511)
                     /locus_tag="ACHLCY_001926"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP0369190.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQECSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIIAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(20604..21251)
                     /gene="rnt"
                     /locus_tag="ACHLCY_001927"
     CDS             complement(20604..21251)
                     /gene="rnt"
                     /locus_tag="ACHLCY_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP0369191.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(21354..21761)
                     /gene="gloA"
                     /locus_tag="ACHLCY_001928"
     CDS             complement(21354..21761)
                     /gene="gloA"
                     /locus_tag="ACHLCY_001928"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP0369192.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(21842..22939)
                     /gene="nemA"
                     /locus_tag="ACHLCY_001929"
     CDS             complement(21842..22939)
                     /gene="nemA"
                     /locus_tag="ACHLCY_001929"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP0369193.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGANEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(22976..23575)
                     /gene="nemR"
                     /locus_tag="ACHLCY_001930"
     CDS             complement(22976..23575)
                     /gene="nemR"
                     /locus_tag="ACHLCY_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP0369194.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            23678..23917
                     /locus_tag="ACHLCY_001931"
     CDS             23678..23917
                     /locus_tag="ACHLCY_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP0369195.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            23966..24862
                     /locus_tag="ACHLCY_001932"
     CDS             23966..24862
                     /locus_tag="ACHLCY_001932"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP0369196.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            24943..25464
                     /gene="sodC"
                     /locus_tag="ACHLCY_001933"
     CDS             24943..25464
                     /gene="sodC"
                     /locus_tag="ACHLCY_001933"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0369197.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(25465..27477)
                     /locus_tag="ACHLCY_001934"
     CDS             complement(25465..27477)
                     /locus_tag="ACHLCY_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0369198.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(27477..28334)
                     /locus_tag="ACHLCY_001935"
     CDS             complement(27477..28334)
                     /locus_tag="ACHLCY_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0369199.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(28337..28573)
                     /locus_tag="ACHLCY_001936"
     CDS             complement(28337..28573)
                     /locus_tag="ACHLCY_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP0369200.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            28774..29208
                     /gene="slyA"
                     /locus_tag="ACHLCY_001937"
     CDS             28774..29208
                     /gene="slyA"
                     /locus_tag="ACHLCY_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP0369201.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(29255..29722)
                     /gene="slyB"
                     /locus_tag="ACHLCY_001938"
     CDS             complement(29255..29722)
                     /gene="slyB"
                     /locus_tag="ACHLCY_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP0369202.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            29996..31105
                     /gene="anmK"
                     /locus_tag="ACHLCY_001939"
     CDS             29996..31105
                     /gene="anmK"
                     /locus_tag="ACHLCY_001939"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP0369203.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            31203..31532
                     /gene="mliC"
                     /locus_tag="ACHLCY_001940"
     CDS             31203..31532
                     /gene="mliC"
                     /locus_tag="ACHLCY_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP0369204.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            31591..32247
                     /gene="pdxH"
                     /locus_tag="ACHLCY_001941"
     CDS             31591..32247
                     /gene="pdxH"
                     /locus_tag="ACHLCY_001941"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP0369205.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            32376..33650
                     /gene="tyrS"
                     /locus_tag="ACHLCY_001942"
     CDS             32376..33650
                     /gene="tyrS"
                     /locus_tag="ACHLCY_001942"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP0369206.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            33709..34572
                     /gene="pdxY"
                     /locus_tag="ACHLCY_001943"
     CDS             33709..34572
                     /gene="pdxY"
                     /locus_tag="ACHLCY_001943"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP0369207.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(34616..35221)
                     /gene="gstA"
                     /locus_tag="ACHLCY_001944"
     CDS             complement(34616..35221)
                     /gene="gstA"
                     /locus_tag="ACHLCY_001944"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP0369208.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(35326..36828)
                     /gene="dtpA"
                     /locus_tag="ACHLCY_001945"
     CDS             complement(35326..36828)
                     /gene="dtpA"
                     /locus_tag="ACHLCY_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP0369209.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         9366 a         9285 c         9685 g         8819 t
ORIGIN      
        1 cctttaaaag catggtcgat ggctatcacc ggcactaacg aatgatcatt acacgagcag
       61 acctgcgaga atggcgtatc ggagcggtga tgtatcgctg gttcctgcgc catttcccac
      121 gcggcggtag ttatgccgat attcatcatg cactgattga ggaaggatat acggactggg
      181 cggagagtct ggttgagtat gcgtggaaaa aatggctggc ggatgaaaac ttcgcgcatc
      241 aggaagtttc ctctatgcaa aaactggcca cagaccccgg agagatacca ttttgcagtc
      301 agtttgcccg cagcgacgat catgctcgca tcggctgttg tgaggataac gcccgaatcg
      361 caaccgctgg gtatgccgcg caaattgcca gtatgggtta tagcgtgcgg attggaagtg
      421 tcggctttaa cagtcacatt ggcagctccg gtgaacgcgc atgggttgct gtaaccggta
      481 attcttcgcg gataagcagc gctggagata gtagtcggat tgcgaatacc ggaatgcgag
      541 tacgcgtttg tacattgggt gaacggtgtc atgtcgccag taatggtgac ctggtgcaaa
      601 tcgccagctt tggcgcaaac gccagaattg ccaacagcgg cgacaatgta cacatcattg
      661 ccagcggtga aaattcaacg gtagtgagca cgggcgtggt cgactcaatt attctcgggc
      721 cagggggaag cgccgcactg gcatatcatg acggcgaaag agttcgcttc gccgtcgcca
      781 ttgaaggtga aaacaatatt cgaaccggag tccgctatcg tcttaatgaa cagcatcagt
      841 ttgttgagtg ctgatagctt tacctttaat caaacgaaag acgccgacga gcacgggatg
      901 caggctttac gaccgcccga gccatcgcct caccaatttc tgcacatgcc gtaagtccct
      961 gaacgaaagg ctggtcgtgc atcaaccagg gcaacgcgcc gaacgctacg cgtccacgaa
     1021 tatcttcggg ttgctgtaac gtataatctt cgccaatatc agggatttca tcccctgttt
     1081 tctgtaattg ctcgcgtagc ccagggaaag gaatatcttt cactttaagc ggacgctgtc
     1141 cgcgggcatc aataaaaacg tcaaacgagt agctgttgtc ttccgttttc aggacggtgc
     1201 gcgactcatt aatttccatt ttgtagtctt caccgagggc gagaatatga atgattcccg
     1261 cttcacgtaa ggcaagtagg cgacgaatag actctgaagg gattgccgca taattatcga
     1321 tgaaaaccct ggcaaggcct ttactgaacc gtttatggtc gtgttcattc agatgaggaa
     1381 caatttcctg tacggcttca tgcaggcgca gaatgacata acgccaggga acagtatgtt
     1441 tttctcgttt attgcgttcg acttcctgga gatttttttc tgcccagtca aatgggtcgc
     1501 gttgtttgcg ctcggcaaac caggcttgag caaaggaatc gacattcagg ctctctaagg
     1561 ctatgcgttg gctccagtct ggatcagcaa acttgatttc ctctactatc aatctaaata
     1621 cccgatccaa aaggccctct tcaccttttt gaatctcagc atttaatgcc tgatcggtga
     1681 cgatgtgtaa gggctcgtag ggaatagggc aatagaaatc ggcttcgggt aaaatacccg
     1741 tgcgcgacat caacgtaata tttagctttt cacttgcgtt atcgcggtta aaaacgacgt
     1801 gttgtttatc atcttcaatg aacgaaccat gctgaatagc cactgccatt gccgcatcca
     1861 gtccgctcaa ggatgttccc ataataccca cgttacacgc atcgaccttt gcttccatca
     1921 ggcctgacca cggactggga aaatacgttc tggttgcttc ttcttcatca ggccagacgt
     1981 gacccgtggc aatcaccgct aaatcaaacg tctcgctggg taaatcctga tttgtagcga
     2041 gcatgacgcc agcatttgta atttgcagat cggtaacctg gcatgattca taaacagcca
     2101 ctgcaaattt ttgctgtcgt gcctggtcta ctaatcgtaa aaattgatcg cggaaatatt
     2161 cgcccagcag aattcgcggt aaaaactgac gatcgtgcaa ggtttctttt ttaacgccat
     2221 aacgctggag atggctggct tcttgctttt gtagccattc gagatacgta caattaatcg
     2281 gcggtatttc aatactggca atatttgcca gcatcatttt tgagttttcc tcatcactgt
     2341 atggcatccc gacaccggcc tcgtcagcct gctcgaagat agaaatagaa agtggagttt
     2401 gttgctgtag aagcgagaat aaggtgtaga tccccgtagg cccggcaccc acaatagcaa
     2461 tttttttcat tgttacttac ctgtacgcat tctttcgaat ttagtgttat cagataagtt
     2521 tggttgagat ttttagaatt tgagaaattt cagacaggta tgaaaagggc gctaaccgcc
     2581 cttgattaaa ttctgtcagg cgagttttgc ctgattgatt tgactcagtg gctcattgac
     2641 atcgaaaact ttggcgacaa cttcctcaac gccgagattt ttaagtcggg cagtatgttt
     2701 ttccagatag gcaagggcgc ttttttcatc agtaaaaaga tagatcccac cggcttcgtg
     2761 gttcttttca ctttctgtcc ataccttcca cagaaaacca ggttcctgat taatcgactc
     2821 agcaagtggt ttaagctgct cagccattgc gtcgccaaat gggccattaa aagcaaaatg
     2881 aagttgtaac aaagttgcca tagcaattct ccttctgata acgcatacga agacagtgta
     2941 aatcttttaa cgtattggat acagagaaga aacagaggaa aaaaggagaa gaccagatta
     3001 agaaaatctg gtgcgtccga gtggactcga accaccgacc cccaccatgt caaggtggtg
     3061 ctctaaccaa ctgagctacg gacgcaggat ggtgcgttca attggactcg aaccaacgac
     3121 ccccaccatg tcaaggtggt gctctaacca actgagctat gaacgcaacg ttgtaggtga
     3181 caacggggac gaatattagc ggcagagtgg gaaggtggca agaggcaaaa cgtaattttc
     3241 tgcgctattt cgaccgtttg cagagctttt aagcaaattg gctatatttt gttgatttgc
     3301 aagggcgatt tttattcagg atcgcattta catctgatac aacccaataa ttcgtgtatc
     3361 agagtttaaa cagagttgtt tgccaccatc tcaaatctgt cgtggataaa tcctccgtag
     3421 cctttacact taggtagcaa taaaggcaca cagatgcagc aaaggaataa atcatgggat
     3481 ctgatgcgaa aaacttgatg agcgacggga atgtgcaaat tgttaagacc ggcgaggtca
     3541 ttggcgcgac gcaacttact gaaggcgagt tgattgttga agctggcgga agagccgaaa
     3601 ataccgtggt cacgggggct ggctggttga aagtggcaac cggtgggatc gccaaatgca
     3661 cacagtacgg taacaatggc acgctatcgg tcagcgacgg tgccattgcc acagatattg
     3721 ttcagtccga gggaggcgca attagtctct ctacgctcgc tacggttaat ggccgccatc
     3781 ccgaaggtga attcagcgtt gatcagggtt atgcctgcgg tttgttgctg gaaaatggcg
     3841 gtaacctgcg tgtactggaa ggacatcgcg cggaaaaaat cattctcgat caagagggcg
     3901 gcctgttggt caatgggaca acctcagctg tcgtggtaga tgaaggtggt gaattgttgg
     3961 tgtatccagg tggggaagcc agcaattgtg aaattaatca gggcggcgtt tttatgctgg
     4021 ccgggaaagc cagtgatacg ttgcttgctg gtggcaccat gaataatctc ggtggtgaag
     4081 actctgacac tattgttgag aatggatcca tttatcgtct ggggacggat ggccttcagc
     4141 tctacagttc cggtaagacg caaaacctgt ccgtgaatgt gggtggtcgg gctgaagtgc
     4201 atgccggtac gctggaaaat gcggtaatac aaggtggaac agtgatcctg ttgtcaccca
     4261 ccagcgcgga cgaaaatttt gtcgtagagg aagatcgcgc accggttgaa ctgaccggga
     4321 gtgttgcatt actggacggc gcttcaatga ttattggcta tggtgcagat ctgcaacaat
     4381 caacgattac tgtacagcag ggcggtgtgt tgattctcga cggcagtacg gtaaaaggtg
     4441 acagtgtcac ttttagtgtt ggtaacatca atctgaatgg cggaaaactg tggctgatca
     4501 ctggtgcggc aacgcatgtg caactgaaag tgaaacgcct gcgcggagag ggagcgattt
     4561 gcctgcaaac cagtgcgaaa gaaatctcac ctgacttcat caatgtgaaa ggggaagtta
     4621 ccggggatat acgcgttgag ataacagatg ccagtcggca aactctgtgt aacgcactga
     4681 aactacagcc agacgaagac gggattggcg caacgctcca gcctgcgtaa aaacacaggc
     4741 gcaacccgtg aaggttgcgc cttgtcttta ttagcgggac gctcgttgca gaatgatggc
     4801 tgacggcaga cgttgcagga accgcatacg cagcatcatc ataatggctg ccgacgtcag
     4861 gccaataata aagcctatcc agaagcctgc tggccccata ggttcaacga ccagatcggt
     4921 cagtgccaga atatagccgc ttggcaagcc cagcacccag taagccgtaa aggtaatata
     4981 gaaaatggaa cgcgtatctt tataaccacg caaaatccca ctgccaatca cctggatcga
     5041 gtcagaaatc tgatataccg ccgccagcaa catcaaatgc gcagccagcg ttacaacctc
     5101 gggattgtcg ttgtacaaca gggcgatttg ctcccgcagt gaaaccgtga atatggccgt
     5161 cagggttgcc atacagacac ccaccataag cccggtccgc gcagcggttt gcgcatccag
     5221 cgttgagccc tgacccagac gataacctac gcggatagtt accgctgccg ccagcgacat
     5281 gggaagcacg aacattagtg aactaaagtt cagggcaatc tggtgtcctg cgacatcaac
     5341 aataccgagc ggagacacta acagagccac gacagcaaac agtgtcactt caaagaacag
     5401 tgccagcgca atcggcaaac cgagttgaat cagtcgtttc ataaccgcag gatcgggttt
     5461 cgcggtgcct ttttcgttac gaatatcgcg catggagcgg gcgcgtttaa tgtaagaaac
     5521 catggcaagg aacataaccc agtacaccgc cgcagtagcc acgccacaac caacgccacc
     5581 gagctcaggc ataccgaaat gaccataaat aaagatatag ttcaccggaa tgttcaccag
     5641 caggccgata aagcccatta ccatacccgg cttggttttt gccagacctt cacactggtt
     5701 acgggcaacc tggaagaaca gatagcccgg cgcgccccac aacaacgcac gcagataacc
     5761 cacggctttg tccgccagag ccggatcgat gttttccatg gagcggataa tgtaacctgc
     5821 attccacagc accagcataa tgagaacgga aacaaaacct gccagccaga aaccttgtcg
     5881 cacctgatgc gcaatgcgct cacgtcgacc ggaaccattt aattgcgcga taaccggcgt
     5941 taatgccagc agcagtccgt gaccaaagag gatcgccgga agccagatag aagtaccgat
     6001 agcgaccgcc gccatgtcgg tggcactata gccgcccgcc atcacggtat cgacaaaacc
     6061 catcgcagtt tgggcgattt gcgcgagaat caccgggatt gctaatgcta ataacagacg
     6121 cgcttcactg atatacttct gcacgtgaac accttttatt tgtagttata tgagagacta
     6181 aaaagccgcc tgaatgggca gcaaaaagaa gaagcagggg aaattcagtc tattgtagcg
     6241 aggatttatt aattctccag tgaaaaaata gtcattctta ccccttcact ggtaagggcg
     6301 tttgccacct gctattgtgc tgaacagaat gtcttaactg atttcaggag ttgtaagtat
     6361 gtttacgggg attgtacagg gcaccgcaaa actggtgtcg attgacgaga aaccaaattt
     6421 tcgtacgcat gtggtggagt tacccgacca catgctggac ggcctggaaa ccggtgcgtc
     6481 cgtggcgcat aacggttgct gcctgaccgt gacggaaatt aacggcaacc atgtcagttt
     6541 tgacctgatg aaagaaacgt tacgcattac caatcttggc gatttaaaag tgggggattg
     6601 ggtaaacgtt gagcgtgcgg cgaaattcag tgatgaaatt ggcggacact taatgtcagg
     6661 tcatattatg accactgctg aagtggcgaa aatattaacc tcagaaaata atcgccagat
     6721 ctggtttaaa gtccaggata gtcagttgat gaaatatatt ctgtacaaag gatttattgg
     6781 catcgacggt attagcctga ccgtcggcga agtcacgcca acgcgttttt gcgtccattt
     6841 aattccggaa acactggaac gcacgactct tgggaagaaa aaactcggcg cacgcgtcaa
     6901 cattgaaatt gatccacaaa ctcaggcagt ggtagatacg gtagaacgtg tgctggcggc
     6961 acgagaaaat gccatgaatc aaccaggtac agaagcctga tctgaactcg cccccgaaga
     7021 gggggcgata gaatagcctt tagcgagcga ctcgaaggcc gttttcaaca ccgcgtgaga
     7081 acaccacctg ccagagctga atatcacggg cgcggaaagc acctgcacaa gcattcagat
     7141 aataggtaaa cattcgttta aagcgttcac tgtagttatc tgcaatttct ggccatgcgg
     7201 cgaggaatcg ttcataccac gccatcaacg tagtatcgta atcagcaccg aagttatgcc
     7261 agtcttccat cacaaagtgg ggttcgctgg actgagcaat ctggcgtaca gagggcaggc
     7321 aaccgttcgg aaaaatatat ttattaatcc agggatcaac attcagatcg gtttttttcg
     7381 aaccgatagt atggagcagg aatatacctt ccggtttcaa attacgatcc accaccgcaa
     7441 aataggtatc gtaatttttc ggtccgacgt gctcgaacat ccccacagaa acaatacgat
     7501 caaactggtc gttcaggtca cgataatctt gcagcaaaat ggtgacatcc aggccttcac
     7561 agcgttcctg agccattttt tgctgttcgg cagaaatggt gacgccgacc acgcttacgt
     7621 catagttaga tgccatgtag tgcgccagtc cgccccagcc gcagccaata tccagtacgc
     7681 gcatccctgg ttttaactgc aatttttcac aaatcatttt gagcttcgcc tgctgggcag
     7741 attccagatt atcggcatct ttccagtaag cgcaggaata ttgcatgaag ggatcaagca
     7801 tgcgactgaa caagtcatta cccaaatcgt aatgctcttt gccgactatc caggcacgtt
     7861 ttttactctg cagattgaag agacgggcgc ctgcaatacg cagcgtgtct ttgaaatgat
     7921 gggggagttg gttctcgaga cctgcgcgta agactttgct aaaaaacata tccagtcggt
     7981 cacattccca ccagccatcc atataacttt cgcctaaccc caaagagcct tcttgcagaa
     8041 cgcgtttaaa aaaatcgggg tttttcacac gaatatccgc cggggcagaa ccgttaatgg
     8101 ctataccggc acggctaagt aattcgttgg cgatacggta ccagttgtca tccggtacac
     8161 tgacttcttc tatacacgat gaactcatag tttctccatc actggtcggt gatcagaacc
     8221 ttaaaacagc gtagacgctt tttgggcttt gtgagaaatc tcgcagaaaa aaccgcgagt
     8281 ctataatccg acgtagaaca gagggaaagg aggaaccctt gccgaaatga ccatccatag
     8341 tgagtcggga acgatcctgt ccccgtaaaa acataatatt tatcgtagct ttaacaactc
     8401 gaaattagta taggccgcta atttcaatca tacaacaaaa ttttgttagc agcctaagta
     8461 taagtatatc gatatagatc agtgtgattc gctatgagcg acttcggcat tgccatgatt
     8521 ctggcagcca acttcttcac aacgttgcat catgtaaccc agcgcgacca gcactactgt
     8581 tgataacatc acgctggtgg tggtgagcaa tggcgtgctg atactgatca gccaggaaac
     8641 taccagactt gcgaggaagc acagacccag ttgcagagtg ttctgcaacg ctgcggcgcg
     8701 accggttgca tgtgggaagg gacgcagcgc ctgggcgaca acaatagggt agatcgcgcc
     8761 attggcaatc gccatcacac agaatgggat caggatttcg accagcgaca catggctaat
     8821 gaagcccgca gcccaggtcg caatgacgct gacagcaaac agcaccagca accacggtaa
     8881 taactgcttg ccttgccatt tctgcagtgc ggcgcgacag ccataaccac caatcagaaa
     8941 cgcgatagtt tgcgggacat aacttaaacc aataactgcc gggctgtagc ccatttcact
     9001 aaggatgaac ggtgaaccgg tcagccatgc aaaaaaactg gctgaacagg ctgcatatat
     9061 cagcacgttg ccgcgatagg ttttagaacg tagcaggtcg gtaaaggtca gaccatcctg
     9121 actattgtta cgggccttcg tcgtgggttt gagccagaaa ataggcagaa tcagcaccac
     9181 ggtaatggca aacagggtgg cgaaaatcgc ctgccaggaa aaatggacca gcagccagct
     9241 tcctaacagg ggagccagtg ccggagacag acccaccagc ggcatgatgg tcgcaaaaat
     9301 acggttgact ttctgtgaag gataataatc tgtcactaat gcttgccaga taaccgccgc
     9361 ggcgcagacc cccacagcct gtacaaaacg caataccagc agcgtagcgg cgttttctac
     9421 ccacagcatc cccagactac ctaacgcaaa aattgtcagg ccgattaata acaccggttt
     9481 acgaccataa cggtcggaga gtggccccca cagaagctgg gctgcggcaa aaccggcaag
     9541 gaacagacta aggctggcac tgacagcaga cgcaggcgtt tgcaggtcgg cctgtatggc
     9601 ggcgaaagca ggcagataca tatcggttgc cagaaaaccg agtacgctca aacccgccag
     9661 ccagactaaa aatcttttcc caggttgcat gtatattctc taaatctctc aattaaacac
     9721 cgccgcaaag tgtagggagt gaaattagtg ttgtgaaacg gtaatatttg atgactggtt
     9781 tcaaaaattt tgcaggcaga ctatgtggtc agaatattca ctcgaagttg ttgatgcggt
     9841 agcgcgtaat ggtagtttta gcgctgcggc acaggagctg catcgcgttc cttctgcggt
     9901 cagctatacc gtgcgtcagc tggaagagtg gctggcggta ccgctctttg aacgacggca
     9961 ccgtgatgtg gagctgaccg ctgctggagc gtggtttctc aaagaagggc gctctgttgt
    10021 caaaaaaatg cagatcaccc gccagcaatg tcagcagata gcgaacggct ggcgcggtca
    10081 gttagctatc gcagtggata atattgtcag gccagaacgt acacggcaga tgatcgttga
    10141 tttttatcgc cattttgacg acgtcgaact tctggtcttt caggaagtgt ttaacggtgt
    10201 ctgggatgcg ctttccgatg ggcgcgtgga actggcgatt ggcgcaacac gcgcgattcc
    10261 ggtaggcggt cgttatgcct tccgggatat ggggatgcta agctggagtt gcgttgttgc
    10321 cagccaccac ccgctggcgt tgatggatgg cccgttcagc gatgatacgt tgcgcaactg
    10381 gccgtcgttg gtgcgcgaag acacctcgcg aacgctacct aaacgtatta cctggttgct
    10441 ggataaccaa aaaagagttg tcgtgcctga ctgggaatca tcggcaacct gtatctcggc
    10501 aggattatgc atagggatgg tgccaacaca tttcgccaaa ccgtggctta atgaggggaa
    10561 gtgggtggca ttagaactgg aaaacccctt tccggattcg gcatgttgtc tgacatggca
    10621 gcaaaatgat atgtcgcctg cgttaacctg gctgcttgag tatctcggcg atagtgagac
    10681 gctgaataag gagtggctgc gggagccgga agagactccc gcaacgggtg attaacgacg
    10741 atagtcgcgg aacgggccgt cagccacgga gcggcgttca atcaagcgcg gatgcacttc
    10801 aatggactgc ggttcttcac gtttgttgac gatacgatcc aacagcatgt tgaacgctgt
    10861 ttcacccagc gaatcttttg gctggtggat cgtggtcagc gccggcgtaa aatagcgggc
    10921 gttgcgcacg ttatcataac cgatcagcga aacatcctgc gggacgcgca gacccatttc
    10981 atcagcagca caaagtgcgc ccattgccat gatatcgcca ccacagaaga cggcagtagg
    11041 gcgatgcggc tgcgacagta tttgctgcat ggcgcgataa ccagattcgg gttcaaagtc
    11101 accctgaaca atccagcttt ccggcacctt gatcatcgct tcttccatcg ccttcataaa
    11161 accggcaagg cggcctgcgc cggtgttacg ttccagcggg ccggggatga cgccgatttc
    11221 gcggtgaccg cgttcaatca gataacgccc ggccatgtag ccgccttcga acgcgttatc
    11281 aatgaccgca tcggtgaagt cagcttttgc ttcaccccag tccatcacca ccattgggat
    11341 atggcgatac tcttccagca tcgccagcaa cggttctggg tactcagaac acatcaccag
    11401 cagaccatcg acgcgttttt gcgccatcat cgacagataa gcccgctgtt tctcaagatt
    11461 gttccacgca ttacccagaa tcagggtgta acctttctgg aagcaatttt tttcaaccgc
    11521 ttcaatgatc tcggcaaaat aggccgcttc gctgctggtc gccagcaaac cgatagactt
    11581 ggtgtggtta accttcaggc tacgcgccac cgcgctaggg gagtagtgta attctttaat
    11641 cgctgcccac acggcgttgc gcgtttcttc agcgacgaaa cgtgttttgt tgattacgtg
    11701 tgacacagtt gtagtggaaa cgtttgctcg tttcgctaca tcttttattg ttgccattcc
    11761 atttcactcc agaccctaac ttcagctcct gcaaaatcgc aaggtaaacg tttgccttta
    11821 cacaccttac gcacaacaaa agtgttgcgg tacgccggaa aaacgacacg gagcgtcagg
    11881 aaggggtcaa tggccagtac gcaaatagtg taagcgtgga attttggccg atcttgacga
    11941 aaaggggaag tggtaaaaac acttatcatt ataaatgacg ggagattttt tcatcacagt
    12001 gtgtaaaaat gcgtaatatc actatttgtg tggtaataaa aaaggagaaa acttgatgag
    12061 caccgacctt aaattttcac tggtaacaac gattatcgtc ctcggtttga tcgtggccgt
    12121 gggtttgact gccgcgctgc actgatttcc tctcgaggga gcgcgttctc cctcgcatct
    12181 ccttcctgtt tgttagcaaa atggcaaaag tgttttctct ttttgttatt ttcatttttt
    12241 tgtgattgct gtcatgcttt cgccacttta atgaatatcg cgtaatgacg cgaagaggtg
    12301 gagataaggc atgaaaatta actatccgtt gctggcgctg gcgattggcg cgtttggtat
    12361 cgggacaacg gagttctcgc caatgggttt gttgcccgtc attgcgcgcg gtgtggatgt
    12421 ctcgattccc gctgccggaa tgttaatcag tgcctatgca gttggcgtaa tggttggcgc
    12481 gccgctgatg acgcttctac tttctcatcg tgcccgccgc agtgcgttga ttttcctgat
    12541 ggcaattttc acgctcggca acgtactttc cgccatcgcg ccggattata tgaccctgat
    12601 gctttcacgc attttgacca gcctgaatca cggagcattt tttggtttgg gttcagtcgt
    12661 ggccgcaagc gtggtgccaa aacataaaca ggccagcgca gttgccacta tgtttatggg
    12721 gttaaccctg gcaaatatcg gtggcgtgcc ggcggcgacc tggttgggtg aaaccatcgg
    12781 ctggcggatg tcatttctgg caacggcggg gctgggagtg atttcaatgg taagtctgtt
    12841 cttctcatta cctaaaggtg gtgcaggggc acgacctgaa gtgaaaaaag agctggcggt
    12901 attaatgcgt ccgcaggtgc tgtctgcatt gctgacgacg gtactgggag ctggtgcaat
    12961 gtttactctc tacacctata tctctccggt actacaaagt attacccacg caacaccagt
    13021 gttcgtcacg gcaatgctgg tgctgattgg tgtcggattc tctatcggta actatctcgg
    13081 cggcaaactg gcagatcgtt cagttaacgg cacgttgaaa ggctttttgt tgttgttgat
    13141 ggtgattatg ctggcaatcc cgttcctggc ccgcaatgag ttcggcgcag ctattagcat
    13201 ggtggtgtgg ggcgcagcaa cctttgcggt cgtaccgccg ttacagatgc gcgtgatgcg
    13261 tgtcgccagt gaagcgccag gtctgtcttc atcagtcaat attggtgcct ttaatcttgg
    13321 aaatgcgctg ggagcagctg ctggtggtgc ggtaatttcc gctgggctgg gatacagctt
    13381 tgtgccggtg atgggagcga ttgtcgcggg actggcatta ttgctggtgt ttatgtcagc
    13441 cagaaaacaa cctgaaacag tttgcgttgc taacagctaa ttaaacaaaa acgcagaagg
    13501 ataaccttct gcgttacagt tgctgacaac gtgcgattta tcttgccgaa tgcgctgtta
    13561 acgcctcatc cgcgttggca tggtaagcga agcgcatcag gcaatgctgc atttgccatc
    13621 agttattatg cagcgagatt tttcgctacg aattcccagt tcaccagcgc ccagaagtgc
    13681 tccagatagc caggacgtgc attgcgatag tcgatgtaat aagcgtgttc ccagacatca
    13741 acggtcagca gcggagtcgc atcggtggtc agcggagtac ccgcgttaga ggttgaaacg
    13801 atagccagtt tgccatcgct gtttttcacc agccaggtcc agccagaacc aaagtttttg
    13861 atcgctgcat cagtaaactg cgctttgaaa tcggcaaagc tgccaaaaga tgcggcgata
    13921 gcttcagcga cttttccagt cggttcgcca ccggcgttcg gtgccaggca gttccagtag
    13981 aaagtatggt tccagacctg agctgcgttg ttgaatacgc caccttcaga gctgcgaata
    14041 atctcttcca gtgatttacc ttcaaacgcg gtacctttaa tcaggttgtt caggttagtg
    14101 acataagtct gatgatgctt gccgtagtga tactcgatag tttccgcaga aatgtgcggt
    14161 gccagagcat ctttagcata tggtagtgca ggtaattcga atgacattgc tactctcctt
    14221 tattattaat ttgcatacgt acaatagcct tattgtgcgt atggataggg tagcaaatta
    14281 aaaggtgaga caaaagatga acttaccctg ttgccgtaac aacagggtaa agttttagcg
    14341 aagtgttttt ggggtcatta cccgacgagc gccaacatag tggcgctgcc agtagtcttc
    14401 actgagagaa gtgatttgaa tttcctgacc tgtgcgcggt gactgaataa atttgccgtt
    14461 gccgacatac acgccgacat gatcggctgt gccgcgtccc tgagtacgga aaaagaccag
    14521 gtcgccgttt ttcagttcac tacgttcgat tggcgctgca tcacgcaggt gatacatttc
    14581 attcgccgta cgcggaatac gaattttcac caaatcttta taagcgtaat aaaccaggcc
    14641 gctgcaatca aaaccggtac gcggtgagct gccaccccaa cgatatggct taccaatttg
    14701 ctgcatcagt ttattcattg ccactttcgt agctttttgc accttcgctt tgtgcgcgtc
    14761 ggcaagagtt cctgacgcgg catttttgac tttcacacaa tgcgatttat agcccttacg
    14821 tttggtacac ttttcggtaa agctggctga ggccgtttta ttgacggcgt gtttgctgcg
    14881 agttttggcg tttttagaag cggtaatgga agatttttta acggaagaag cggttttgct
    14941 tttggtcgta cttttactgg cggctttttt cgctgttttc tggctggctt tttgggtctt
    15001 tttggtggtt gccgtgcttt tctttttatc tgcttttttg gtgatatgag tggtagcaga
    15061 actctccctg gcttgctttg aagcatgggc cataggcgtt aaaggcaggg tggtaaaaag
    15121 taaagcacag agcgtgatcg aaatacggtt tatccgcgcc actgagcgtc cctctgttaa
    15181 ggcaggtcta tcatgcctgc gtctgattta caaaacttga tgattctaat gcatagaaga
    15241 agagaaagtt aatagctttt attgttaaaa aatacgtttc gttggcgagc gcaaaaaaat
    15301 gcagcagtga aagctaaaaa tggttataaa tacgccagtt tgccgagcat cagggaagaa
    15361 acgaatattt aaaatgcaac aaatagagca aagggataaa atagcaaaag cgtgatgaaa
    15421 atgttatttt ccgatgccgc actgacccgc tacaatgtca gattactgcc gtaaagaagt
    15481 ttgaggaagc aagacaatga gcaccactat cgaaaaaatc caacgccaga ttgctgaaaa
    15541 cccgatcctg ctgtacatga aaggttcacc gaaactgccg agctgcggtt tctctgccca
    15601 ggcagtccag gcgcttgccg catgtggcga acgttttgcc tatgttgata ttctgcagaa
    15661 cccggacatt cgtgcggaac tgcctaaata tgctaactgg ccgaccttcc cgcaactgtg
    15721 ggttgacggc gagctggtag gcggttgtga tatcgtgatc gaaatgtatc agcgtggcga
    15781 actgcagcag ctgatcaaag aaactgccgc taaatacaag tctgaagagc cggacgcgga
    15841 ataatccgtc ctgcaatact gacggatgcg aacgggcatc cgtcagtcct ttctctatcc
    15901 ccaatccagc ccttgtggcg aacttgaaaa tcccgcctca cgcagcagcg taaacatcgg
    15961 cgtttgccgg accggtagat cattcacttc tgttagcgta aagcgcagcc gtggttcgcg
    16021 acgcagtgcg gtagtcagcg cgtggaaaac ctctggggcg agtaattcct ctttttcctg
    16081 ccagaccagc atttttttgc caccttgcgc cagatagagt aacaatttgc cgccagaaac
    16141 gaccaccagc gcacccgccc gacgcgttgg aaccagcgtt gccggatgtg caggccaggg
    16201 cagaagattt ccccacacat tagccggatc gttggcagag agcgccactg gtgtatagtg
    16261 gcgcgtttgc gtggcttgtg tcgccagatc gcgcaatcgg tcaatagtca gacgttcagc
    16321 gaattgcgcg ccacccagac cttctacaaa acgacctcgc ataattcgcc cggagtcttc
    16381 catacttcga caaagcgttt gcatcgatgg aaacccgcca gggatatttt cggctatcac
    16441 cgcctgacga ctgatgatgc cgtagcggtc gagcatattt tccgccagcg ccagcatcct
    16501 ttcggtatcg tttagtggct ccacctgcaa taacgaccag cgtccagcca gatttggtgt
    16561 gttgtaagat accagcggcg agacgggttg cgcatagaca ggacgtccac gacgagcccg
    16621 gtgactgcgg cgagttgagg tgcgtgcgtt ggaactgctg cgggtgaggg cgcgtaacgg
    16681 tgcccaaatg tcgctggtga tgacgccttg ccagaccagc gcccataacg cctcttgcag
    16741 ggcagagaga tcaaccgatt cgccgatttt gtcgcgtatc cgttggctga tttgttgtgc
    16801 aaaccaggct cctccgtcag ccagaacagc gattatcgct tgctgcagcg ccgaacgatt
    16861 cgcctgatcc gcttcggcgg gagtgaacga ttctgcggca tattcctgta gatgcagtgc
    16921 caccaggccg tcatcttcac ccagcttttt ttgtcccgac cagataaccg ctccggttgc
    16981 cagtaattca tcgagcattt ctggcgaata gtcgcgtacg cgggcaggca ggatctggct
    17041 ttcccagagt gaggcgggta aaccgactcc ggcaagctgt tcgatcaccc gcatcacgcc
    17101 atctacgccc tcataaacgc ctggcgatgt tgaggcaaag agcgccgggc taccatcggt
    17161 ggcgggtaat acgccctgac gttccagcag caatcgcgca taggtcgtgg ctgcaacggg
    17221 acgcgtcgct tctctggcgg cttgcagcga gcgcaaacgc agacgacgaa atacttcatc
    17281 gctcacccag atgtcttgtt gcagattcat caccagaccc tgttcacgca gttgctgaag
    17341 ctgctcttcg acaatggcaa taccgagact aaactcatga gccagttgtt cagccgtgac
    17401 cagagcatga gcccggagat agcgcagaaa gaggtcgcga agcgggtaac tgactctatg
    17461 taaataaatc tctggcaatg actctggtag tcgtacgccg agggcatcac gcagcctggc
    17521 ggcatcatcc atacaggcaa gacgctcctg tccgctaatc atcgctggga agattcgttt
    17581 tactgcaaga agattttcca gatacgacgc aacctcttca ctgctgcctg tatgccgttg
    17641 cgccaggtct tcaacggtca ttggccccag ttcgcgcagc aggtcgaaca atccttcttc
    17701 acctttcgct cttctgccag gagccagtcg ttgcaactct tcttccacct ggcgaatgac
    17761 ctgcgggtcg agtaattccc ccggatcgac ctgtccgagt agattgcgca gtaactcgct
    17821 gtccagcgac agtacggatg cccggcgctc tgccagcggg gcgtcgctct ggtacataaa
    17881 ttccgcgaca tagccgaaca ataaacttgt ggcgaaaggc gagggcgtag tggtcgttac
    17941 atcggatatt tgaatttcgc caccgttcag gcgacgcatc aaacgttcca gtgcgggaag
    18001 atcataaaca tcttgcagac attcgcgtag ggtttcgaga atgaccggaa aatccggata
    18061 tccctgagcg atttccagca actgactggc gcgcagccgt tgttgccaaa gcggggtgcg
    18121 atggcccgga gtgcgccccg gcattaataa tgcccgcgcg gcgcattcgc gaaaacgggc
    18181 ggcgaaaagt gccgagctgc ctaccgcctc gcggacaatt tgcagcaact tttctggttc
    18241 aaacaaaaaa atcgcggcgt cgggcaattt accatcagtg tcaggaatgc gcgcaacaat
    18301 gccgtcatca ctggcgacca cagacgcgtc agcgccccat agggcatgta ttcgcccggc
    18361 aatcgccagc gcccagggtt catgcacccg tcttccatag ggagagtgca aaataatacg
    18421 ccagtcgcca atttcatcac ggcaccgttc gaggaccaga tggcgactcc ccggaacgat
    18481 gcccgtcgcg ttgcgttgct cgtcaatcaa cccctgaata ttggcgttcg tattttcttc
    18541 tgccagccac gggggaatag tcccggaaaa gaacgcgcca tccgccagta aatgaagaaa
    18601 atcgccgatc atctcgccta attcagccgg acgtccgtta ccttcaccac gccagaaggg
    18661 gagccgggca gaacgacccg gagcaggagt cacaatcacc tgatcgcggg tgatttgctg
    18721 gatccgccat gaggtagcgc cgagagtgat aatgtcgttc acccgcgact catataccat
    18781 ctcctcatcc agttcaccca cccgccgcga accggcctgt tcttcacctt cgggtaataa
    18841 cacgctatac atgccacgat ccggaatggt gccgccgctg gtaaccgcca acaattgagc
    18901 gccaggtcgg gcggtcaata tcccggtctc cctgttccag accagtttgg ggcgaaaagc
    18961 agaaaaatcg ccagagggat agcgcccgga aagcatatcc agcgtggcgt caaaaacacg
    19021 tcttggcaga tctttccacg gtgcggcacg gcgtacgcgg gagtaccatt cgtctacctg
    19081 taatgcatcc atcgccgcgg cggcaacggt ttgctgcgca aggacgtcga gtggattatg
    19141 cggcggtgtc aggttttcca gcctgcctgc gaacatacac tctacaatga ctgcggaatc
    19201 gactaagtca cgccgggtac gagggaaaaa cagcccttta gatacaccgc caacctgatg
    19261 tccggcgcga ccaatgcgtt gtaacccact ggcaacagaa agcggcgttg ccacctgaat
    19321 caccagatcc accgcgccca tatcaatccc cagttcgaga ctggaggttg cgaccacgca
    19381 gcgtaattca cccgatttca gcgcctgttc ggtgattgct cgttgttctt tagagacgga
    19441 gccgtggtgt gagcgggcaa taaaaacgtc gctactttgt acatggttag aggttgcgcc
    19501 ggaggtcgac tcgaaatggg ccgtatcaac ggcgatagac ggggaacgct gtaagcgtgc
    19561 ggcgtaaagc tcatttaatc gtgccgtcag tttttccgcc agcccacgtg aattagtaaa
    19621 gacaatggtc gagcgatggc gcaacacttc atcaaggata cccgtttcaa tatatggcca
    19681 gatggagcct tcccggccgg catggctgtc ttcgccggtg ccgctggcga ccgatgagac
    19741 atcatccata ttggcgaccg gtacgacaat tcgtatctgc ggatggcgca ttgcgggcgg
    19801 gttgactacc gtaaccgggc gatcgccacc aagaaatgct gccacatcgc tggctgagcg
    19861 cacagtggca gaaaggccaa ttcgctgtgc tgaggtgtgg agcagcgcat cgagccgctc
    19921 cagacttaac gccagatgcg caccacgttt actgcccgct accgcgtgga cttcatcaat
    19981 aattaccgtt tcgacgccgc gtagcgtttc gcgcgcgcgg gaggtcagca tcagatagag
    20041 tgattcgggt gtggtgatca gaatatccgg cggattacgg gtgagtttgc tgcattcctg
    20101 tgcaggcgta tcgccagtac ggatccctac gcgaagattg acttccgttt cgccgcgccg
    20161 ccgccgttca tcggcaatac ccttcaacgg gagctgcaag ttgcgctgaa cgtcggtgcc
    20221 cagagctttt atcggtgaaa tatagaggat gcgtgaggtt ttacgcttat gcgcctcgcg
    20281 ggtatcttcg ccgccttcgc ggaagagccg atcgagggcg tagagaaatg ctgccagcgt
    20341 tttcccggag ccggtcggtg caatcaccag cgcatgttcg cttcgcgccg ccacatgcca
    20401 ggtttgcgac tggacagcgg tcggctgttt aaaggcgcga agaaaccagt cgcgggtcgc
    20461 cggtgaaaac acgtccggca ggagcgatga agggtcagga ttatctgcca ttaacgctat
    20521 tccccgtgaa tatcatgctt gatgcgtgga atagcctcac tatagatgcc tgaatcattg
    20581 catgtcatca ggcatcgact cgattacacc tcttcggcgg caggtagcgg ccagcctccc
    20641 agacgtttcc agcggttgac gatttcacaa aacagcacag cagtgcgttc ggtgtcgtac
    20701 agcgcggagt gcgcctgggt gctgtcgaag tccatgccag cggtctggca agcctttgac
    20761 aatacggttt gtccgagtgc cagcccggcc agtgcagcag tgtcaaaagt ggcgaaaggg
    20821 tggaacgggt tacgtttcag tgaggcgcgt tctgcggcgg ccatcataaa gctgtgatca
    20881 aaattggcat tgtgcgccac cataatggcg cggttacagc cgctcgcttt aataccttta
    20941 cgtacaactt taaaaatttc gtgcagcgcc tcgtattcgc tgaccgcgcc gcgatcggga
    21001 tcgttcgggt caatgccgtt gaaggcgagg gcctctggct gcaaatttgc gccgacaaat
    21061 ggttcgacgt ggaaatgtaa tgtggtgtcc ggcatcagcc agccttgttc atccattttc
    21121 agggtgatgg cggcaatctc aagcagcgca tcggttttgg cgttaaatcc ggctgtttca
    21181 acatcgatca caacaggata aaaaccacga aaacggtcgc acagaccggt aagttgagcg
    21241 ttatcggaca tctgattctc ttaatacgga taaattgcag cgcgcattat gacaaatatt
    21301 acttgtcgat gcagtaaaga tgcgggcgcg atgagttcac gcccggcagg agattagttg
    21361 cccagaccgc gaccggcgtc tttctcttcg attaactcaa ttttgtaacc gtccggatct
    21421 tccacaaacg cgataaccgt agtaccgcct tttaccggac ccgcttcacg ggtcacgtta
    21481 cccccgtttt gacggatttt ttcgcacgct tcagcggcgt tatctacgct aagcgcgatg
    21541 tgaccataag cagtgccgag ttcgtattta tccacgcccc agttgtaggt cagttcaatc
    21601 accgcttctt cggtttccgg gccgtagcca acaaacgcca gtgagtattt gtattccggg
    21661 ttttcgctgg tacgcagcag tttcatgccc agcactttgg tataaaaatc gatggagcgt
    21721 tgcagatcgc caacgcgcag catggtatga agaagacgca tctttttatc ctcaaaatgg
    21781 ctaacagatt caaaatgttg ttttagtata gcggcggctt tacgccgctc gcaatgttgg
    21841 attacaacgt cgggtaatcg gtatagcctt ccgcgccgcc accgtagaaa ctttcggcac
    21901 gctgtgggtt aagctcagct ttgcgctgca agcgggcgac cagatccggg ttcgcaatcc
    21961 agtcacgacc aaatgccacc gcatcaatta accctttgcc gatcagcgtt tcagcttttt
    22021 ctactgtgta tgcacctgcg ccgataatcg gaccgtggaa acgggcgcgt actttttcgc
    22081 ggaacgcatc agtatacggt tcaccccccg cccaatctgg ttctgacata tgcagataag
    22141 caatgccgcg tttacccagt tgttcaatca gatacagtgc atcggcttct tcattcgcgc
    22201 cgttatccgt gttctggaaa gtaccgattg gcgaaacgcg aatgccaatg cgatcggcac
    22261 cccattcttc aatcccggca tcgaccactt ccagtaccag acgtgcgcga ttttccacgc
    22321 tgccgccgta ctgatcggta cgatggtttg aagaaggaga aaggaactga tgcagcaaat
    22381 aaccgtgagc agagtggagc tctaccagat caaaaccggc ttcacgcgcg ttagcaatgg
    22441 cctgacggaa atcattgacg atacctggaa tctcttccag ttcaagcgca cgcggcatgg
    22501 atgtttcaac acggatcgcc tgaccatttt catcgcgcag agaagtacgt gttcccgcgc
    22561 taagtgctga aggcgctacc ggtgcctgac cgccaggttg caggctggcg tgagaaatgc
    22621 gtccggtgtg ccacagctgc acggccatat gaccattttc agcatgaacg ccagcggtga
    22681 tttttttcca tgcggcaatt tgctccggac tatggatgcc aggcgcacct gcatatcctt
    22741 ttgcctgggc agaaatttgc gtggcttcac taataatcaa accggcactg gcacgttggc
    22801 gatagtattc cgccatcaac ggggtaggaa tgtcacccgg ttcaatactg cgcagacgcg
    22861 tcagcggtgc cataaaaata cggtttgccg ccgtgatcgc gcccactttc agtggggaat
    22921 acagtttttc agatgacata aaggctcccg gtcgtctggt gataaaaaat gcctgctaaa
    22981 cggcaggcgt cgcaataatg ttttttacat gggccagcgc gttttccagt ggctcgaaac
    23041 tgcgcgaaat tttggcctgc agattcgcac ccagccatag tgcgtaaagc acttgtgcct
    23101 gttgtagcgg ttcgccacaa aaggttaaac aatggttctc acggccattt tccagcgcct
    23161 gagagagcag ggcgatcacg ccgcgagcac ctttatccat cgcgctgcgc atatcttctg
    23221 acagatcgca cacttcggca gagagtttta ctgtcaggca accactgatg gttccatgtt
    23281 ggcaaaactg gttcagtgtt tgctggtaat aagccagtat gcggtcgcgg tagttacctt
    23341 cgccggattg cagcaactca gtcagtcgct ggtgatatgc ggcgtaatga cgctcaagca
    23401 tggcaacgcc aaacgcttct ttagagcgaa agtagtgata gaaggaccct ttcggcactt
    23461 cagcggtttt tagtaattcg cttagcccca tcccggtgaa tccacgttgc aggcaaagtt
    23521 gctcgcccgt cgccaggaga tgttcgcgag tatcatgttc ggtgtgtttg ttcatgcgtt
    23581 ggagtgtagt agaccagtcg gtctagtgca agcaaagttg ctactttgga cattaacagc
    23641 ttcaataatg gtaataatct tatctgagga gggcgaagtg gcggagcaat tagagttctt
    23701 tcccgtccag agcccgtgcc ggggaatttg ccagtctgac gaacgcgggt tttgtcgcgg
    23761 ctgttttcgt agtcgtgatg agcgttttaa ctggaataaa atgagcgatg gcgaaaaaca
    23821 agaggtgctg cgcctttgtc ggcagcgatt aatgcgcaaa ttacgcgcaa ataagccagc
    23881 gtcatcagac gaaccggagc aaccatcact cttttgaacc taatcttgcg tatactcctc
    23941 tcatatctga taagaggaag tgattatggt tcagcgtatt actattgcgc cgcaaggccc
    24001 ggagttttcc cgttttgtga tgggctactg gcgattgatg gactggaata tgtccgcccg
    24061 ccagctggtc agttttattg aagagcatct ggatctcggc gtgaccaccg tggaccatgc
    24121 tgatatttat ggtggctatc agtgcgaagc ggcgtttggc gaggcactga aactggcacc
    24181 tcacctgcgt gaacggatgg aaatcgtcag taaatgcggt atcgcgacga ccgcgcgtga
    24241 agaaaacgtc attggtcatt acatcactga ccgcgatcac atcattaaga gcgccgaaca
    24301 gtcgctaatt aatctcgcga ccgatcatct ggatctgctg ttaatccacc gaccagaccc
    24361 gttaatggat gccgatgaag tggcggacgc gttcaaacat ctgcatcaga gcggcaaagt
    24421 gcgtcatttt ggcgtatcga actttacgcc tgcgcaattt gccctgttgc aatcacgtct
    24481 gccgtttacc cttgccacta atcaggtgga aatatccccg gtgcatcagc cgttactgct
    24541 ggatggcacg ctcgaccaac tacaacaact gcgtgttcgt ccgatggcgt ggtcctgcct
    24601 tggtggtggt cgtctgttta atgatgatta tttccagcag ctgcgtgatg aactggctgt
    24661 ggtggcagag gagttaaacg cgggctcgat tgaacaggtg gtttacgcct gggtattacg
    24721 tttaccatcg cagccgctgc caattatcgg ttcaggtaaa attgagcgcg tacgggcagc
    24781 tgtcgaagca gaaacactga aaatgacccg tcaacaatgg tttcgtatcc gtaaagcggc
    24841 actggggtac gacgtaccgt aagcactttt aggaatagcc gccgttcaaa aatgtgtcac
    24901 tggtttacac ttattcagga atgcacaatg aacggaggtc ctatgaaacg ttttagtctg
    24961 gctattctgg cgctggttgt tgcaaccggc gcacaagctg ccagtgaaaa agtcgagatg
    25021 aacctcgtca cgtcgcaagg ggtagggcag tcaattggta gcgtcaccat tactgaaacc
    25081 gataaaggtc tggagttttc gcccgatctg aaagcattac cccccggtga acatggcttc
    25141 catattcatg ccaaaggaag ctgccagcca gccaccaaag atggcaaagc cagcgccgcg
    25201 gaatccgcag gcgggcatct tgatccacaa aataccggta aacatgaagg gccagaaggt
    25261 gccgggcatt taggcgatct gcctgcactg gtcgtcaata atgacggcaa agctaccgat
    25321 gccgtcatcg cgcctcgtct gaaatcactg gatgaaatca aagacaaagc gctgatggtc
    25381 cacgttggcg gcgataatat gtccgatcaa cctaaaccgc tgggcggtgg cggtgaacgc
    25441 tatgcctgtg gtgtaattaa gtaattaaga ggccagcgta ccttgcggtg gtgcttgctc
    25501 aagttgcgaa agcgagcagt acagccgcca gacaattgcc gccagctcac gggcggcagg
    25561 ttgatgatga cgggcaaggc tgtcgcaaat ccgctgtaat tcttcaagtg tggccgccag
    25621 tgatttttgc tgaacgccac gctcactcat cactccccgc aacagtgaaa tacagttatc
    25681 ccgtactcgc gataacggat cggaacgcga ttcccagtcg cgcaattgcc agacaacatg
    25741 agaacagttc agcagcacta caccccagcg taataaccaa cggcgagcca gcgcatcctg
    25801 gctgttactc aactgactga cgtgatgata agtgagcgat tcaaattcgc tttcactcag
    25861 tgttggatgg cggcttagct gatcgacaaa atcccggcgc agcgcgcgaa tatggcggcg
    25921 gcttttacga gcatccgatc ctggacgcag aatggcgaac gctagccaag ccagcgcaac
    25981 gccaacgatt tttgccagat tatcgttaag aaaatcagca aaatcataca ccgggggatt
    26041 agtgacagcg ataaacgaac ccataaaaac aatcagttgc ccccacagtg cggcaaattt
    26101 gggcatctgc aatttaagaa gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa
    26161 ttgccacaga tcgctaatct ggaccatcag gccaaatttg accacaaagc tgaatagcga
    26221 aagtaacacc agcgtgcgca tcagcagtga caacgactta aacggtgctg cgacggcgga
    26281 gtagagtacg cagctaattg ctgccagcgt taatgcattg gcaccggcat cccattgcga
    26341 tgcaatactc catgcgccaa tcatcatcaa cgtacaaaat gtacgcagcc cgctccacat
    26401 agcttcggcg ttgtcggtat gacgagccag agcggatgtc cgtgcgagtc tggcatgatc
    26461 atctacaccg ctttgcagac gatgtaattc ctgactactt tgcagataaa ggcggcaaaa
    26521 ataacgtagt cgctgccaga aggcgacgtg ccggtagtcg gcgacgttgg tcgggcgtag
    26581 cggggcgata atacgtgcga cggtgtaagc atctgtttgc gaactggcga gcgccgtcag
    26641 caattgttcg agaatttctc gcgtggcacc tggcggtgag ggccagttga gcaacatacg
    26701 tcgcaggctg gagatgacac tggtcatacg taattgctgg tggagtagcg cattaaggcg
    26761 cgcgttttgc tggcgaaaac gatagtggct ccagaaagcc tggatacgca gcaaattcat
    26821 ggtcagtatc tgcccaatca ccccctcatg tgctgcacga atggcatcgt ttgtttcagg
    26881 ctgccagagt aaactggcat gttccagtaa tcgggcgtgc atgtttttca atgcggttaa
    26941 aagggctgta gcatcggaac tgctaggcag gatcatcatc atcatgccgc cgcacaaaat
    27001 accgactatc acctcgcaaa cgcgcgcctg agcgatatcc cacagctggc tggcctcagt
    27061 aatattaacc atcggaaagg cgatgatggc agccgtgtag cccgccagtt gaaatgcata
    27121 cgcgacgtta ttcgtgaagt gcgcacaggc ccaggtacaa aagccaagcc acgccgacat
    27181 gctcaataga aaaaaccacg gctcattgag cgtatgcccg gcaagaagca gtgccgcaat
    27241 ggctcccagt aaactgccag cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt
    27301 gggaaagcta accactgcag ccgaggtcat cgcccaatag ggttcatcca gattcaaata
    27361 ataggcaacc gtcagcgcca gacacatggc aatggtattg cgtaacgcat aacgccattg
    27421 cgccagcgtg gccttgaacc agggcaaatt gcgcaaggac catgacgatg cgttcattat
    27481 tgacctacgg caatgctaca ggtggtgccc gacaccagcg tgacatcgcc tggaacttta
    27541 tccagcgcaa agcggacggg aacgcgttgg gcgagacgaa cccaggggac gttaggtttc
    27601 acatccggaa taaggcttga gtcactttca acgctttgat cataaatcgc ccgaccgata
    27661 ctggaaacgt gaccccgtaa cgttttgtta tcactataga gggtaatttg tgctggtgca
    27721 ccttcgcgga tatggcgcaa cttggtttct tcaaaataac caatgacgta aaacgagtgg
    27781 ctgtcgacaa gggcaaacag cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg
    27841 aggttagtca cccaaccact caccggggcg cgaatttccg tttgcgccag ttgccattgt
    27901 gtctgtttca gcgtggcctg tgcggcatta acactggcct gcatcgcttt aacattgagg
    27961 ttagcggtat cgagttcttc ggcagaaata aaattttgtg agagatgacg acgacgattc
    28021 gcttcgttat tggcttttgc gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc
    28081 agctcggcga tctgaaaggg cgttttatcg atcgtgagca gaagatcccc cgcgttaacc
    28141 agttggttgt cttttatatt cagctcaaca atacgtcccg acacctgtgg ggtgatagaa
    28201 accttttctg cgcgtatttt cccgtcgcgt gtccacggag attgcatgta ataattccac
    28261 agccaccatc cggcaagaac cgcaactatc gctacaatga ttgttgagaa atacttaatt
    28321 gttttaattg acatagttac cacgcaatca gtattgccag agcaaggcaa acgcaaatcg
    28381 caaacagcga taaatccatt aacaagggat gccagatgtc accggcgtag atccagccac
    28441 gaagcaggcg gtgtacgaca agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg
    28501 gaggaaaata gacggacgca ccgaacacca gatcttgtaa gggcaatcct gttgcattga
    28561 gcataaactt cacaaagtag attcctttac gaccgaatat attgcgttag attaataaat
    28621 attctttaag tgcgaaaaat ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa
    28681 atgcagcaat acatttgttt tagcaataca attgctgcac actattctaa aagccgcata
    28741 atatcttagc aagctaatta taaggagatg aaattggaat cgccactagg ttctgatctg
    28801 gcacggttgg tgcgcatatg gcgtgctctg atagaccatc gcctgaaacc gctggagtta
    28861 acacaaaccc attgggttac gttacacaat atccatcagt tacctccaga ccagtcgcaa
    28921 attcaactgg caaaagcgat tggcatcgag cagccatcac tggtccgtac tctggaccaa
    28981 ctggaagaaa aagggttaat ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt
    29041 attaaactga cggaaaaggc agagccactg atcagcgaaa tggaagctgt tattaacaaa
    29101 acccgcgcgg aaatattaca tggcatctcc gcagaggaac tggagcaact gattacgctc
    29161 atcgcaaaac ttgagcataa tatcattgag ttacaggcca aagggtgaaa tgaagggggc
    29221 gtgtggtcag taacctgacc acacgcaaac ttatttagcg cggagaaacg gtcacctgac
    29281 tgccattgct ggccagtacg acacgttggc ccggagagaa acgagtgttg ccttgtttct
    29341 gtaccaccat gatggtatta ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt
    29401 tgttcattgc actctgtacg ccctgaccag ctacgccacc tgcaacagcg cctgctgcag
    29461 tagccagaga acgcccggtt ccgccaccaa cagtattccc caggaaacca ccaagaacag
    29521 caccgccaat tgcaccgata acgttggaat catcaccgcc ctgaatctgt accggacgta
    29581 cgttaacgat ggtgccatag ctgacattct gtacttgttt cgcttcagaa gcggtataaa
    29641 catcccctga cagggtgtcg ttattaacac aaccgacaag agacagacct accattgaaa
    29701 caaccaatac gcgtttaatc attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc
    29761 atccctcatg gtcaaagtat atggcatatg tgaatccatg gttacatgtt ctaccaaata
    29821 tgagcaaatc ataatgaaag taggatcaac caattataaa caaaacattc atgatgatta
    29881 ttgatttgaa gataatgttt tactaccaac ggtaaaaatc ggtcattccg catggtattc
    29941 acgaaaagtt tatgtttgag tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa
    30001 atcgggccgc tttattggcg ttatgtcagg caccagcctt gatggtgttg atgttgtgtt
    30061 ggcgacaata gatgaacacc gggtcgcaca gctggcaagc ttgagctggc cgatcccggt
    30121 gtctctgaaa caggctgtac tggatatttg ccagggccag cagcttacac tttcgcagtt
    30181 tggacagctt gatactcaac tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa
    30241 ggaacaaaac ctgcaggcga gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg
    30301 gcatgaaccg acgggcgtgg aaccacacac tttacagatt ggcgataaca atcaaattgt
    30361 ggcacgcacc ggaattacgg ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg
    30421 acaaggcgca ccgctggtac ctgcgttcca tcatgccttg ctggctcacc caaccgagcg
    30481 acgaatggtg ctcaatattg gcggcatcgc caatctgtca ctgctcattc ctgggcagcc
    30541 ggttggcggc tacgataccg gtcctggtaa catgctgatg gatgcctgga tctggcgtca
    30601 ggccggtaaa ccttacgata aagatgccga gtgggcacgg gcgggtaaag ttattctccc
    30661 actgctgcaa aatatgctca gcgacccata tttctcgcaa cctgcaccga aaagcaccgg
    30721 acgcgaatac tttaactacg gctggctgga gcgccattta cgccattttc cgggtgttga
    30781 tccccgtgat gttcaggcca cactggcaga actcaccgcc gtgaccattt ctgaacaagt
    30841 tttgttgagc ggtggctgcg aacgattgat ggtatgtggt ggaggtagtc gtaatccgct
    30901 actcatggcg cgtctggcgg cattactgcc aggcacagaa gtcaccacca ccgatgccgt
    30961 tggcattagt ggcgatgaca tggaagcatt ggctttcgcc tggcttgcct ggcggacgct
    31021 ggcgggatta ccaggaaatc tgccttccgt cactggcgca agccaggaga cggtactggg
    31081 ggctattttc cctgctaacc cgtgatatca gagttaactg aatttttctt accgcgtctg
    31141 ccgttacact tggaacgtta gggagggcgt attgccctcc agaccaggaa agtcttcggg
    31201 aaatgtctat gaaaaaactg ttactgatct gtttgccggt gcttctcacc ggctgtagcg
    31261 cgttcaatca actggttgag cacatgcaaa ccgctacgct ggaataccag tgtgatgaaa
    31321 aaccgttgac ggtcaaactg aataatccgc gccaggaagt cagttttgtt tacgataatc
    31381 aactactgca tctcaaacag ggcatttcag cgtctggtgc acgttacact gacggaatct
    31441 atgttttctg gtcgaaaggc gatgaagcga ctgtctataa acgcgaccgc atcgtattga
    31501 ataactgtca gttacaaaat ccacagcgtt gagatttttc caggggcggc gcacaatagc
    31561 gtcacccact gacaatccgt aaagaaaacc atgtctgata acgacgaatt gcagcaaatc
    31621 gcgcatctgc gccgtgaata caccaaaggc gggttacgcc gtcgcgatct tcccgccgat
    31681 ccattaaccc tttttgaacg ttggctctct caggcgtgtg aagccaaact ggcagaccct
    31741 accgcgatgg tggtcgctac cgtggatgaa catgctcagc cttatcaacg catcgtttta
    31801 ctcaaacatt acgacgaaaa aggcatggtg ttttacacca acctcggtag ccgtaaagca
    31861 catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc
    31921 caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat gaaatatttt
    31981 catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc cagtcgcatt
    32041 tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt tcaacagggc
    32101 gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca gattgagttc
    32161 tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgaga aaatgatgcg
    32221 tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta atcgctggta
    32281 ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg
    32341 gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac ttgattaaac
    32401 aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta gcagagcgac
    32461 tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac agcttgcatt
    32521 tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc cacaagccgg
    32581 ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa gctgccgagc
    32641 gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt aagcaggttg
    32701 ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac aactatgact
    32761 ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta
    32821 accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat caggggattt
    32881 cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt ctgaacaaac
    32941 agtacggtgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc acttctggta
    33001 tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt ccgctgatca
    33061 ctaaagcaga tggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga
    33121 agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat gccgacgttt
    33181 accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc ctggaagaag
    33241 aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag caggtgactc
    33301 gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa tgcctgttca
    33361 gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag gacggcgtac
    33421 cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc
    33481 aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc attaacggtg
    33541 aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt ggtcgtttta
    33601 ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa tgcattaagt
    33661 ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat gatgaaaaat
    33721 attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt
    33781 cctatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac
    33841 acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac cgaaattgtg
    33901 caaggcattg ccgccattga taaattacac acctgtgatg ccgtgttaag tggctatctg
    33961 ggatcggcgg agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa agccgcgaat
    34021 ccgcaggcga aatatttttg cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt
    34081 gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg
    34141 ccgaatctgg ttgagctgga aatactctgt gagcatgcgg taaataacgt cgaagaagcg
    34201 gttctggcag cgcgcgaact cattgcgcaa gggccacaaa ttgtgttggt taaacacctg
    34261 gcgcgagctg gctacagccg tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagcc
    34321 tggcatatca gccgtccgct ggtggatttt ggtatgcgcc agccggtagg tgttggtgat
    34381 gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca ggaggcgctg
    34441 gaacatgtga ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat gcaggaatat
    34501 gagctgcaag tggtggctgc tcaggatcgt attgccaaac cagaacatta cttcagcgca
    34561 acaaagctct gaaatatctc atttcggccc acaacgacct gtgggccgat ggatattact
    34621 ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata cgttgcataa
    34681 atgctgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa
    34741 ccgtaaacag ataggcatca gcaattgtaa atctttgccc gcagatccag tgctcatcct
    34801 tcagtgcctc gttcacatat tgcagcttct tctccagctg cgcgcgaact gtcggtttgt
    34861 actcttccgg tgtatcaggg cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga
    34921 tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt gccagcaact
    34981 ggcggtcggg gacgctgtcg gcaagatact gcataatcgc tacgccttcc gtcagcaaag
    35041 taccgtcatc cagcagcaat gcaggcacct gccccttagg gttaacggca aagtaatcgt
    35101 caccgttttc gagacgtttt ttcattaaat ccacactgac gagggtaaaa tcctttccgc
    35161 tctcacgcag ggtgatatgg gaagcgagag agcaggcacc cggtttgtag aacaatttca
    35221 tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa caaaaaaaag
    35281 ccgctaacga aaagttagcg gcttgtaaaa gagtttccct gaattttacg ctacagcagc
    35341 tttcgccgct ttgtctgcag cgtcatcctg cgtcatacgg tgcagtttag gcgcggtcag
    35401 cagcatcagt actgcaataa tggcagtagc gacaccaatc tgcaagaata cgcgaccata
    35461 aacttccagt gacatcagcg ggtcggtaac gttatccggc acagccatca tacccgcaac
    35521 ataaccacca atcaggtttg caccggcagt ggtcaggaac cagctaccca taatgaagcc
    35581 catcagacgc tgcggaacga gttgtgcaac cattgccaga cccagaccag agatcatcag
    35641 ttccccgatg ctctgcaggc catagcttgc gaccagccag cttacagaca cgataccagc
    35701 gtcagacgcg aatttcgcac ccagcggcag aatcaggaac gcaccagaac acatcaccat
    35761 gccgattgca aacttggttg gcatcggcag ggtatcgccc atcttgttat agatagcggc
    35821 cagaatcgga ctaccgatga tgatccagaa cgggttcagt gcctgatact gctcaggttc
    35881 tacggccaga cccagaatgg agtgctcaac gttacgaatc gcaaagaagt tcagtgatgt
    35941 cggcatctgg ctgtacagca cgaagaagat gattgcttcg agcatcagga tgaaggcaac
    36001 gatcatttta cgacgcgcag cacctttcat cgcgaaggct tctttaccga agataaccac
    36061 gataccgaag gcaacaacgc ccagcgccat acgcgcaact tcctgattgt gcagcagcca
    36121 ggtggcgata gcgatcagtg ccacaacacc aataatggtt agcagcaggt tacggtagtt
    36181 gataggctcg aagtctggtt ttgaaccgta ctgtttaacc cagcgttggc agaaggcgaa
    36241 gttaacgata gtgatcagca ggcctacaac gctcaatgca aacgcaacgc tccagccgta
    36301 tttcgcggcc agccacggcg tagcaatcat agagaagaaa gagccgatgt tgacggacat
    36361 gtagtacatg gtgaatgcac cgtccagacg cgggtcgttt ttctcatagc atgtagaaag
    36421 cagagaagac gggttagcct taaacaggcc gttaccgacc gcaatagccg ccatacccat
    36481 ataaacaata ccggcgtcgt gaccagacca ggcaaccaga gcataaccaa tcgccagcac
    36541 aatagcgccg agcataatta cgcgtttagt acccagtacc ttgtcaccta accagccgcc
    36601 gatagcgacc agaccataaa ccagggcact aaaggaagag aaaagggtga ttgaatccgc
    36661 ttcagacata cccagttgtt taaccaggta aacagccata attccttgta ggccgtaata
    36721 accaaaacgt tcccataact caatcgagaa gatgagatag aacgccttcg gttgtttgaa
    36781 agcgttcaaa ctgacgcttt cagttggttt ttggtttgca gtggacactt ttacctcttt
    36841 ttttacatcc catattaacg ggggtgcttt ctcgcgttat gtctaaaaaa catacgcgca
    36901 attgttatag tgggagggga aacggcaggt aatgttcact atcttgcact ttcagacaag
    36961 tcctttgtaa taatctgtta catataactg aggcggggaa ttcaaccatt ccgggaaatg
    37021 ttatccagcg ttaaattttt caaaaaacgt aacggcagat tttttcactg cactgagagg
    37081 ggggtgatgg cttaacggcg atatgttctg ctatttagcg tttttactag tgagatagtc
    37141 cagtttctga aaaat
//
LOCUS       DAZGTR010000035        35502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGTR010000035 DAZGTR010000000
VERSION     DAZGTR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.035"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            128..670
                     /locus_tag="ACHLCY_001946"
     CDS             128..670
                     /locus_tag="ACHLCY_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0369210.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            753..1454
                     /gene="elfD"
                     /locus_tag="ACHLCY_001947"
     CDS             753..1454
                     /gene="elfD"
                     /locus_tag="ACHLCY_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0369211.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1479..4079
                     /gene="elfC"
                     /locus_tag="ACHLCY_001948"
     CDS             1479..4079
                     /gene="elfC"
                     /locus_tag="ACHLCY_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP0369212.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            4070..5140
                     /gene="elfG"
                     /locus_tag="ACHLCY_001949"
     CDS             4070..5140
                     /gene="elfG"
                     /locus_tag="ACHLCY_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0369213.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNEGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5152..5694
                     /gene="ycbU"
                     /locus_tag="ACHLCY_001950"
     CDS             5152..5694
                     /gene="ycbU"
                     /locus_tag="ACHLCY_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0369214.1"
                     /translation="MKKKTIYQYVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5702..6217
                     /gene="ycbV"
                     /locus_tag="ACHLCY_001951"
     CDS             5702..6217
                     /gene="ycbV"
                     /locus_tag="ACHLCY_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0369215.1"
                     /translation="MLKRIIWILCLLGLTGSSELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGMDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6183..6920
                     /gene="ycbF"
                     /locus_tag="ACHLCY_001952"
     CDS             6183..6920
                     /gene="ycbF"
                     /locus_tag="ACHLCY_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0369216.1"
                     /translation="MPARHLYFIMTNTWNRLTLLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            7031..8041
                     /gene="pyrD"
                     /locus_tag="ACHLCY_001953"
     CDS             7031..8041
                     /gene="pyrD"
                     /locus_tag="ACHLCY_001953"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP0369217.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8215..8757
                     /gene="zapC"
                     /locus_tag="ACHLCY_001954"
     CDS             8215..8757
                     /gene="zapC"
                     /locus_tag="ACHLCY_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP0369218.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8754..9863)
                     /gene="ycbX"
                     /locus_tag="ACHLCY_001955"
     CDS             complement(8754..9863)
                     /gene="ycbX"
                     /locus_tag="ACHLCY_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP0369219.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PGAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10107..12215
                     /gene="rlmKL"
                     /locus_tag="ACHLCY_001956"
     CDS             10107..12215
                     /gene="rlmKL"
                     /locus_tag="ACHLCY_001956"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP0369220.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLGGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12227..14134
                     /gene="uup"
                     /locus_tag="ACHLCY_001957"
     CDS             12227..14134
                     /gene="uup"
                     /locus_tag="ACHLCY_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0369221.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14264..15517
                     /gene="pqiA"
                     /locus_tag="ACHLCY_001958"
     CDS             14264..15517
                     /gene="pqiA"
                     /locus_tag="ACHLCY_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP0369222.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15522..17162
                     /gene="pqiB"
                     /locus_tag="ACHLCY_001959"
     CDS             15522..17162
                     /gene="pqiB"
                     /locus_tag="ACHLCY_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP0369223.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17159..17722
                     /gene="pqiC"
                     /locus_tag="ACHLCY_001960"
     CDS             17159..17722
                     /gene="pqiC"
                     /locus_tag="ACHLCY_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP0369224.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17978..18145
                     /gene="rmf"
                     /locus_tag="ACHLCY_001961"
     CDS             17978..18145
                     /gene="rmf"
                     /locus_tag="ACHLCY_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP0369225.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18215..18733)
                     /gene="fabA"
                     /locus_tag="ACHLCY_001962"
     CDS             complement(18215..18733)
                     /gene="fabA"
                     /locus_tag="ACHLCY_001962"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP0369226.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18802..20562)
                     /gene="ycbZ"
                     /locus_tag="ACHLCY_001963"
     CDS             complement(18802..20562)
                     /gene="ycbZ"
                     /locus_tag="ACHLCY_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0369227.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLSDFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20748..21200
                     /gene="matP"
                     /locus_tag="ACHLCY_001964"
     CDS             20748..21200
                     /gene="matP"
                     /locus_tag="ACHLCY_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP0369228.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21276..22316)
                     /gene="ompA"
                     /locus_tag="ACHLCY_001965"
     CDS             complement(21276..22316)
                     /gene="ompA"
                     /locus_tag="ACHLCY_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP0369229.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22673..23182)
                     /gene="sulA"
                     /locus_tag="ACHLCY_001966"
     CDS             complement(22673..23182)
                     /gene="sulA"
                     /locus_tag="ACHLCY_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP0369230.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23446..24030
                     /gene="sxy"
                     /locus_tag="ACHLCY_001967"
     CDS             23446..24030
                     /gene="sxy"
                     /locus_tag="ACHLCY_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP0369231.1"
                     /translation="MATLGTIEYRSLFGSYSLTVDDTVFAMVSDGELYLRACEQSAQY
                     CVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYSLDAALKEKSTRNTRE
                     RLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQNSLVTEKILFMLEGAI
                     IGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23993..26155)
                     /gene="yccS"
                     /locus_tag="ACHLCY_001968"
     CDS             complement(23993..26155)
                     /gene="yccS"
                     /locus_tag="ACHLCY_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP0369232.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26165..26611)
                     /gene="yccF"
                     /locus_tag="ACHLCY_001969"
     CDS             complement(26165..26611)
                     /gene="yccF"
                     /locus_tag="ACHLCY_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP0369233.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26734..28788
                     /gene="helD"
                     /locus_tag="ACHLCY_001970"
     CDS             26734..28788
                     /gene="helD"
                     /locus_tag="ACHLCY_001970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP0369234.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28820..29278)
                     /gene="mgsA"
                     /locus_tag="ACHLCY_001971"
     CDS             complement(28820..29278)
                     /gene="mgsA"
                     /locus_tag="ACHLCY_001971"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP0369235.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29374..30036)
                     /gene="yccT"
                     /locus_tag="ACHLCY_001972"
     CDS             complement(29374..30036)
                     /gene="yccT"
                     /locus_tag="ACHLCY_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP0369236.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30209..30622
                     /gene="yccU"
                     /locus_tag="ACHLCY_001973"
     CDS             30209..30622
                     /gene="yccU"
                     /locus_tag="ACHLCY_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP0369237.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30667..30984)
                     /gene="hspQ"
                     /locus_tag="ACHLCY_001974"
     CDS             complement(30667..30984)
                     /gene="hspQ"
                     /locus_tag="ACHLCY_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP0369238.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31042..32232)
                     /gene="rlmI"
                     /locus_tag="ACHLCY_001975"
     CDS             complement(31042..32232)
                     /gene="rlmI"
                     /locus_tag="ACHLCY_001975"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP0369239.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32327..32605
                     /gene="yccX"
                     /locus_tag="ACHLCY_001976"
     CDS             32327..32605
                     /gene="yccX"
                     /locus_tag="ACHLCY_001976"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP0369240.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMLWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32602..32931)
                     /gene="tusE"
                     /locus_tag="ACHLCY_001977"
     CDS             complement(32602..32931)
                     /gene="tusE"
                     /locus_tag="ACHLCY_001977"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP0369241.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(33022..33681)
                     /gene="yccA"
                     /locus_tag="ACHLCY_001978"
     CDS             complement(33022..33681)
                     /gene="yccA"
                     /locus_tag="ACHLCY_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP0369242.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(34089..35104)
                     /locus_tag="ACHLCY_001979"
                     /pseudo
     CDS             complement(34089..35104)
                     /locus_tag="ACHLCY_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(35082..35324)
                     /locus_tag="ACHLCY_001980"
     CDS             complement(35082..35324)
                     /locus_tag="ACHLCY_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP0369243.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(35392..>35502)
                     /locus_tag="ACHLCY_001981"
     CDS             complement(35392..>35502)
                     /locus_tag="ACHLCY_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0369244.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
BASE COUNT         9134 a         8437 c         9190 g         8741 t
ORIGIN      
        1 aaattaaaga tttgtcactt attggattta gtatgcttga tttaaatggc ggtcaattat
       61 tgttattatt aagtctaatg tcatttaaag gcattatcat atttacgaag agatcaggat
      121 gatgacgatg aaaaaaagtg tattgacggc gtttataact gtggtatgtg caacgtccag
      181 cgttatggca gctgatgata atgctatcac ggatggcaaa gtaacattta atggtaaagt
      241 tattgctcca gcttgtaccc tggtagctgc gacgaaagat tcagtagtca cattgccaaa
      301 tgtcagcgcc accaagttgc aaactaatgg agcagtctct ggcgttaaaa ctgatgtacc
      361 aattgcctta gaaggctgtg atgttactgt aactaaaaat gcgacgttta cctttagtgg
      421 tacggctgac ggcgtgcaac ctacagcatt tgccaaccag gctaccaccg atgcagcgac
      481 gaatgttgct ctacaaatgt atctgccaga tggttctaca tcggtaactc ccggtaccga
      541 aacgtcgaat attcagttag cggattctgc tgagcaaaca gtgaccttta aagttgatta
      601 tatcgctacg gggaaagcga cttctggtaa tgtgaatgcg gtaacaaatt tccatattaa
      661 ctattattaa tagaactcat taattgtttt attaattagt acccctctag tgttctggag
      721 gggatattca tattttttaa gagtgactat ttatgaaaac ttgcataaca aagggaattg
      781 tgaccgtaag tttaacggca atattactca gctgctcatc cgcatgggcg gcaggtaaag
      841 gcgggattgg acttgcagca acacgtcttg tttattcaga aggtgaagag caaatttcac
      901 tgggtgtacg taacaccagt ccggatgttc cttatcttat tcagtcatgg gtgatgaccc
      961 cagataataa aaaatcagca gattttatta ttacaccacc gctatttgtg ctgaatccgg
     1021 ctaatgagaa tctgttacgc attatgtaca ttggtgcgcc gttggcgaaa gacagagaaa
     1081 cccttttctt cactagcgta cgggcagtcc cttcaacaac gaagcgggaa gagggaaata
     1141 ccctgaagat tgccacacaa agcgtcatca aacttttctg gcgaccaaaa ggtttagcgt
     1201 atcccttagg cgaggctccg gcgaaactgc gttgcacttc gtcagctgac atggttacgg
     1261 tcagtaaccc aacaccttat ttcattaccc tgacagacct gaaaataggt ggaaaagtag
     1321 ttaaaaatca aatgatttcc ccctttgata aataccaatt ttctctgcca aagggggcca
     1381 aaaatagcag cgtaacgtat cgaaccatca atgactacgg ggcggaaacg ccgcaactca
     1441 actgtaaatc gtaagccgtc ttcagttaag agagcgagat gtatagaact catcgacaac
     1501 acagcctgtt aagctctggt ggagtgccat cgtttattgg tgggctggtg gtgtttgtgt
     1561 cggcagcgtt caatgcacaa gctgaaacct ggttcgatcc tgcttttttc aaagatgatc
     1621 cctcaatggt ggccgatttg tctcgtttcg aaaaaggaca aaaaataacg ccaggggttt
     1681 atcgtgtcga tattgttctg aatcagacaa ttgtagatac gcgcaacgtc aattttgttg
     1741 agataacgcc agagaagggg attgccgcct gtttgacgac tgaaagcctg gatgcaatgg
     1801 gtgttaatac tgatgcgttt ccggctttta aacaactgga caaacaagcg tgtgtgccat
     1861 tggcggagat tattccggat gccagcgtaa cgtttaatgt gaataaactc cgtctggaaa
     1921 tttcagtacc gcaaatcgcc atcaaaagta atgcccgtgg ttatgtcccc cctgaacgtt
     1981 gggatgaagg gatcaacgcg ctattactgg gatattcgtt tagcggggct aacagtattc
     2041 atagcagcgc agacagtgat tctggcgaca gctattttct gaatttaaac agtggcgtta
     2101 atttaggccc atggagattg cgcaacaatt caacatggag tcgcagtagt ggccaaaccg
     2161 cagaatggaa gaatctcagc agctatttgc agcgggcggt tattccacta aaaggcgaac
     2221 tgaccgtagg tgatgattat actgcaggcg attttttcga tagtgtcagc tttcgtggtg
     2281 tgcagctggc gtcagatgac aacatgctgc cagacagcct gaaagggttt gcgcctgtgg
     2341 tgcgtggtat cgccaaaagc aatgcccaga taacgattaa gcaaaatggt tacaccattt
     2401 accaaactta tgtatcgcct ggtgcttttg aaattagtga tctctattcc acgtcgtcga
     2461 gcggtgattt gttagttgaa atcaaagaag cggacggcag cgtcaatagc tacagcgtac
     2521 cgttttccag cgtgccatta ctccagcgtc aggggcgaat caaatacgcg gtgacactgg
     2581 cgaaatacag aaccaatagt aatgaacagc aggagagcaa atttgcccag gccacgttgc
     2641 agtggggcgg accgtgggga acgacatggt atggtggtgg acaatatgct gaatattacc
     2701 gtgccgccat gtttggtctg ggatttaacc ttggcgattt cggagcaatt tcgttcgatg
     2761 cgacccaggc gaagagtacg ctggcagacc aaagcgaaca taaaggtcag tcatatcgtt
     2821 ttctgtatgc caaaacgctc aaccaattgg gcactaattt tcaattgatg ggctatcgct
     2881 attcgacgtc gggtttctac accctttccg acaccatgta taaacatatg gatggctacg
     2941 aatttaatga cggtgatgat gaagatacgc cgatgtggtc gcgttattac aatttgtttt
     3001 acaccaaacg tggcaaactg caggtcaata tctcccagca attaggcgag tacggttcgt
     3061 tttatttaag tggtagccag caaacttact ggcataccga tcaacaggat cggctattac
     3121 agtttggcta caacacgcaa attaaagatc tctcgctggg ggtttcctgg aactacagta
     3181 agtcccgtgg tcaacctgat gctgatcagg tgtttgcact aaatttttcc ctgccgctca
     3241 atctgttgct ccccagaagt aatgatagct ataccaggaa aaaaaattac gcctggatga
     3301 cctctaacac cagtatcgat aacgaagggc acattacaca aaacctgggt ttaacggaga
     3361 cactactcga tgacggtaac ctgagctaca gcgtgcaaca gggatataac agcgagggga
     3421 aaacggctaa tggtagcgcc agcatggact acaaaggggc gtttgcagat gcccgagtgg
     3481 gctacaacta cagcgataac ggcagtcaac aacaactgaa ctacgctctt tcaggcagtt
     3541 tagttgccca ttcacagggc attaccctgg ggcaatcgct gggggaaact aacgtgctga
     3601 ttgcagcacc aggcgcagag aatactcgtg tggcgaacag caccgggctg aaaactgact
     3661 ggcgcggata taccgttgtc ccctatgcca cttcttatcg ggaaaatcga atcgcacttg
     3721 atgcggcatc gttaaaacgt aacgtggatc ttgaaaatgc agtagtcaac gtggttccca
     3781 ccaaaggggc gttggttctg gcggagttca atgcccatgc gggagcaagg gtattaatga
     3841 aaacatcaaa gcagggtata ccgctgcgtt ttggcgcgat agcgacgctg gacggcatac
     3901 agactaatag cggcattatt gatgatgatg gctcgctcta tatgtccggt ttgcccgctc
     3961 agggagcgat aactgtacgc tggggcgaag ctcccgatca aatttgtcat atcagttacc
     4021 agcttaccga acaacaaatt aactctgcga ttacgcgaat ggatgccata tgcagataat
     4081 ctttggagaa aaatgcgtgt tattactacg actatttttt gccgccgtct taatgctatg
     4141 gtgcgctcaa actgctgctt atagcgggca gtgtcatacc actcagggga atccgtatat
     4201 tggcgtcaat tttggcgtta aaaccctgga ggaagaagaa aatacggctg gagtagttaa
     4261 agacaaattt tatcagtgga acgaatcgaa tgattattat gtttcctgtg attgcgataa
     4321 agacaatgtc agaagtggcc gatgggcatt cgccgcggat tcaccgttag tctatttagg
     4381 cgacaactgg tacaaaatta atgactatct tgccgccaaa gttttattgc aggttaaagg
     4441 cagttctcct actgcggttc ctttcgaaaa cgtgggcaca ggggcagata cacgatggca
     4501 tatttgcgat cccggcggtc aacgtttagg tggccagggg gctagcggta atagcggtag
     4561 cttttccctg aaaatattgc agccgttcgt tggttcggtc gttattcctc ctatggcgct
     4621 ggcgcgatta tttgaatgct acaacatacc cgcaggtgat tcctgcacga ctacaggtac
     4681 accggtttta gtgtattacc tgtctggtac gatcaattca cttggctcat gttccgtcaa
     4741 tgccggagag actatcgagg tcgatctggg cgacgtattt gcggctaact ttcgtgttgt
     4801 agggcataaa ccccttgggg ccagaacggc agaactcgca attccagtca ggtgtaacac
     4861 gggaaacgaa gggttagtta acgtcaacct gagtctgacg gcaacctcag accccagcta
     4921 tccccaggcg attaagacgt cacgtcctgg cgtgggcgtg gtggtgaccg atagccagaa
     4981 caacattatt tcccctgctg gtgggacact accgctctct attcctgatg atgcagacag
     5041 tatcgcgcga atgaatgtct atccagtcag cacgacaggc gtaccaccag aaaccgggcg
     5101 atttgaagcc acggcaacgg tgagaataaa ttttgattaa cgcgtgaacg tatgaagaaa
     5161 aaaacgatat atcagtacgt tattttgttc tttagccttc ttaacatcca tgtcgggatg
     5221 gctgggcctg aacaagtcag tatgcatatt tatgggaatg tggtcgatca gggctgtgat
     5281 gtcgccacca aaagtgcatt acaaaatatt catattggcg attttaatat cagtgatttt
     5341 caggccgcga ataccgtaag cactgcagct gatttgaata tcgatatcac cggttgtgcc
     5401 gctggtatta ctggcgcgga cgtccttttt agcggcgagg ctgacaccct tgccccgaca
     5461 ctgcttaaac taactgacac aggcggaagc ggtggtatgg caacggggat tgccgtgcaa
     5521 attcttgatg cgcaaagtca gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc
     5581 ttaaaagccg gggataacac actcaaatat caacttcgtt ataagtccac aaaggcggga
     5641 gcaacgggcg gtaatgcgac ggcggttctc tattttgatc tggtttacca gtgaaggagt
     5701 gatgttgaaa cggataatct ggattctgtg cttactggga ttaacgggga gtagtgagtt
     5761 atttgctcat gatggcacgg tcaacattag cggatcgttt cgtcgtaata catgcgtgct
     5821 ggcacaggat agcaagcaaa tcaatgtgca gttaggcgat gtctcgctga cacgtttttc
     5881 tcacgggaat tatggcccgg agaaatcttt catcattaat ttgcaggatt gcggaacaga
     5941 tgtcagcacg gttgacgtca ccttttcagg aacccccgac ggagtgcaga gcgaaatgct
     6001 gtccattgaa agtggtatgg atgcagccag cgggctggca attgcgattc tggatgatgc
     6061 gaagatattg attccgctta accaggcgag taaagactac agcctacata gcggtaaggt
     6121 gccattaact ttttatgcgc aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt
     6181 gaatgccagc gcgacatttg tacttcatta tgactaatac gtggaacaga ttgacgctct
     6241 tgattttcgc cgttttatcg ctgctggtgg cgggtgaatt gcgggcagga gtggtggtcg
     6301 gcggaacgcg atttatcttt ccggcagaca gagaatcgat atctatttta ctgactaata
     6361 ccagtcagga atcctggctt attaacagta aaatcaaccg cccaacgcgt tgggccgggg
     6421 gggaagcgtc gacagtgcca gcaccattac tggccgctcc gccacttatt ctcctgaagc
     6481 ccggtactac aggcacgttg cgcttgctga gaacggaaag cgacatcttg cctgtggatc
     6541 gcgaaacgct atttgagtta agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga
     6601 gcgtaaaagt ggcgatgcgc tcggtattta aactgttctg gcgacccgaa ggtttgccgg
     6661 gcgacccgct ggaagcttat caacaattac gctggacacg gaattcgcag ggtgtacaac
     6721 tcactaaccc aacgccttac tacattaacc tgattcagtt gagcgtaaat ggtaaagcgt
     6781 taagtaatgc gggagttgtg ccgcctaaaa gccagcgtca gacaagctgg tgtcaggcaa
     6841 tcgcaccgtg tcatgtcgcc tggcgggcga taaatgatta cggcgggttg agtgcaaaga
     6901 aggagcaaaa tctgccctga aacaggttcg gaaaacgttt gcgttttttt tgccgcaggt
     6961 caattccctt ttggtccgaa ctcgcacata atacgccccc ggtttgcaca ccgggaatcc
     7021 aggagagttc atgtactacc ccttcgttcg taaagccctt ttccagctcg atccagagcg
     7081 cgctcatgag tttacttttc agcaattacg ccgtattaca ggaacgccgt ttgaagcact
     7141 ggtgcggcag aaagtgcctg cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc
     7201 gcttggtctg gcagccggtc ttgataaaga cggggagtgc attgacgcgt taggcgcgat
     7261 gggatttgga tcgatcgaga tcggtaccgt cacgccacgt ccacagccag gtaatgacaa
     7321 gccgcgtctc tttcgtctgg tagatgccga aggtttgatc aaccgtatgg gctttaataa
     7381 tcttggcgtt gataacctcg tagagaacgt aaaaaaggcc cattatgacg gcgtcctggg
     7441 tattaacatc ggcaaaaata aagatacgcc agtggagcag ggcaaagatg actatctgat
     7501 ttgtatggaa aaaatctatg cctatgcggg atatatcgcc atcaatattt catcgccgaa
     7561 taccccagga ttacgcacgc tgcaatatgg tgaagcgctg gatgatctct taaccgcgat
     7621 taaaaataag caaaatgatt tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa
     7681 gatcgcgccg gatctttctg aagaagaatt gatccaggtt gccgatagtt tagttcgcca
     7741 taatattgat ggcgttattg caaccaatac cacactcgat cgttctcttg ttcagggaat
     7801 gaaaaattgc gatcaaaccg gtggcttaag tggtcgtccg cttcagttaa aaagcaccga
     7861 aattattcgc cgcttgtcac tggaattaaa cggtcgctta ccgatcatcg gtgttggcgg
     7921 catcgactcg gttatcgctg cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat
     7981 ttattctggt tttattttta aaggtccgcc gctgattaaa gaaatcgtta cccatatcta
     8041 attatttctt cgaattcctc tttataagat aatcagggct ttattttcag ccctggttgt
     8101 tttatattca tcgctgttgc ttatttagac attttgtact tttattgttg aggttattaa
     8161 gcgaagcgac aatggattgt gttgctgcgg ttttataggg taggggagag gcagatgcga
     8221 attaaaccag acgataactg gcgttggtat tacgatgaag agcacgatcg aatgatgctc
     8281 gatttagcca atggtatgct atttcgctca cgttttgcgc gcaagatgtt gactccagat
     8341 gccttttctc ccgcaggctt ttgcgttgac gacgccgcgc tctatttctc ttttgaagaa
     8401 aagtgccgcg attttaattt atctaaagaa caaaaagccg aactggtgct gaatgcactg
     8461 gtagcaattc gctatctcaa accgcaaatg ccgaaaagct ggcattttgt ttcccatggt
     8521 gaaatgtggg tgcccatgcc gggcgatgcg gcctgcgttt ggttgagcga tactcacgag
     8581 caggtcaatt tgctggtcgt tgaatctggc gaaaatgccg cactatgcct gctggcacaa
     8641 ccctgcgttg ttattgcggg tcgcgcgatg cagttgggcg acgccattaa aatcatgaac
     8701 gacaggctga aaccgcaggt taatgttgac agcttcagcc tcgaacaggc agtctaacgc
     8761 gccaacttaa gtgcagtctt cggtacacag ctacagcaaa gaatggtgcc atcatcgccc
     8821 attgctgatt tttttagcgg cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca
     8881 caaatgcccg cgcggcaaga ataagggata cgaatcccct gattttctaa ttgctccagc
     8941 aacacctgtt ggttatttcc acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg
     9001 tccggttgtt gcgtgatgtt ggcggtatca tcagcggcag ctgcgccgta aatttttgcc
     9061 ggagccgttg ccagaatttc cacctcatcg ccaacgcgaa tcacgccgct attacgggca
     9121 attaaattct gaccaaaatc gacatcgccg ttatcctggg cagtgcggaa agattgcaat
     9181 gtttttaatg gttcgcctgc cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa
     9241 atacagcggc tacaaggttt aaccacatca aacaccacat caccaatgcg aatcactttc
     9301 cagcgatctt cttcccaggc tgacgcgccg gaaaccacca gattggggcg gaattgctcc
     9361 atttttacac tggccggaca acgttgttgg agatcacgta acgaggcttc gttagcaaga
     9421 aggtaaggat agccatcagc aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg
     9481 gtcatttgtg gccccaccca gcgtaattgc acttcgcggg agaaaaatcc acttagccat
     9541 ttgttgatcg cgcctggcgc aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt
     9601 gcgtcttgtg tggcgaaatc agcaaaacga acatatgcgc tactgccatc tggtgcggtg
     9661 agatgcaagc catcatgcac aggtgaagga gtaaaccgta ccatttgcgg aaactggcga
     9721 gcagtaataa acgtaccatc aggttccgtg atcataaaga tgcgatcgaa ggccagacca
     9781 ctcacatctg ccagggtatg tgtaagacca atgccgcgca tcgatttaac aggatgaata
     9841 aaaagccggg ttaatgtcgc cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta
     9901 tgacatgacg cgcttattag ctataatgcg caacaatttt cttagcgcct gtcccgttag
     9961 gctattttat ttgtcagttt ggcccaggga agtgctcgaa atcctcacgt acttgtgtac
    10021 gctccggttt ctccgcgctg tccatgtcca gactgactgc aacaattacg cctactgcgg
    10081 taggctctta gagtaaaagt gacgatatga attctctgtt tgccagtacg gcccgtgggc
    10141 tggaagagct gttaaaaact gaactggaaa acctgggggc cgttgaatgc caggtggttc
    10201 agggtggggt ccatttcaag ggcgacacac ggcttgttta ccagagcctg atgtggagcc
    10261 gcctggcctc gcgtattatg ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc
    10321 tctatctcgg tgttcaggcg atcaactgga cagagatgtt taatcctggc gcgaccttcg
    10381 ctgtccactt cagtggtttg aatgacacca tccgcaacag tcagtacggc gcgatgaaag
    10441 tgaaagacgc gatcgtcgat gctttcacgc ggaaaaatct gccgcgtcca aatgttgatc
    10501 gcgatgcgcc ggatatccgc gttaacgtct ggctgcataa agaaaccgcc agtatcgctc
    10561 ttgatctgag tggtgatggt ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc
    10621 cgatcaaaga aaccctggca gccgctattg tgatgcgatc cggctggcag ccaggaacac
    10681 cgctgctcga tccgatgtgt ggttccggta cgttgctgat tgaagcggcg atgctggcga
    10741 ccgatcgcgc accaggcttg caccgtgggc gttggggctt tagcggctgg gcgcagcatg
    10801 atgaagctat ctggcaggaa gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctgg
    10861 ctgagtatag ctctcatttt tacggttcgg acagcgacgc acgggtgatt caacgtgcac
    10921 gcactaacgc ccgtcttgcg gggattggtg aactgatcac ctttgaggtg aaagatgtcg
    10981 cgcaactgac caatccgctg ccgaaagggc cgtacggcac agtgttgagc aacccgccat
    11041 acggggaacg tctggacagc gaaccggcgc tgattgcgct gcatagcctg ctgggtcgga
    11101 tcatgaaaaa ccagtttggt ggctggaatc tctctttgtt tagtgcctcg ccggatctgc
    11161 taagctgctt gcagctgcgt gcagacaaac agtacaaggc gaaaaacggc ccgctggact
    11221 gcgtacagaa aaattaccac gttgccgaaa gcactccaga cagcaaaccg gcgatggtag
    11281 cggaagacta cgccaaccgc ctgcgtaaga acctcaaaaa attcgagaag tgggctcgcc
    11341 aggaaggaat tgaatgttac cgcctgtatg acgccgatct accggaatat aacgttgccg
    11401 ttgaccgtta tgccgactgg gtggtggtgc aggagtatgc gccgccaaaa actattgatg
    11461 ctcacaaagc gcgtcagcgt ctgttcgata ttatcgctgc aaccatttcg gtactgggga
    11521 ttgcgccaaa caaactggtg ctgaaaaccc gtgaacgcca gaagggcaaa aatcagtacc
    11581 agaaactggg cgagaagggc gagtttcttg aagttaccga atataacgct cacttgttgg
    11641 tgaacctgac ggattacctc gataccggtc tgtttctcga tcaccgtatc gcccgtcgta
    11701 tgctcggcca gatgagcaaa ggcaaagatt tcctcaacct gttctcttat accggcagcg
    11761 ccaccgtgca tgcgggatta ggcggtgcac gcagcaccac caccgtggat atgtcgcgta
    11821 cttatctgga gtgggcagaa cgcaacctgc gtctgaatgg cctgaccggg cgtgcgcatc
    11881 gcctgattca ggccgattgc ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga
    11941 tctttatcga tccgccaacc ttctctaact caaaacgaat ggaagatgcg tttgatgttc
    12001 agcgcgatca tctggcgctg atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga
    12061 tcatgttctc gaacaacaaa cgtggcttcc gtatggatct cggcggcctg gcaaaactgg
    12121 gactgaaagc acaagaaatt acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc
    12181 agatccacaa ctgctggctg attaccgcag cctgaaagga atagtaatgt cattaatcag
    12241 tatgcatggc gcctggctgt cgttcagcga cgcgccgctt ctcgataacg cagaactgca
    12301 tatcgaagat aatgaacgtg tttgtctggt aggccgcaac ggcgcaggca aatcgacgtt
    12361 aatgaaaatc ctcaaccgtg aacaagggct ggatgacggt cgcattattt acgagcaaga
    12421 tttgattgta gctcgtctgc aacaggatcc gccgcgtaac gttgagggta gcgtttatga
    12481 tttcgttgcc gaaggcattg aagaacaagc ggaatatctg aaacgctatc acgatatttc
    12541 gcgcctggtg atgaacgacc cgagcgagaa aaatctcaac gaactggcga aggttcagga
    12601 acagctggat caccacaact tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca
    12661 actggggtta gatcctaatg ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc
    12721 ggcattagga cgcgcgctgg tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa
    12781 ccatctggat atcgaaacca tcgactggct ggaagggttc cttaaaacct tcaatgggac
    12841 gattattttc atctcccacg accgttcgtt tatccgcaat atggcgacgc gcattgttga
    12901 tctcgatcgc ggcaagctgg tgacctatcc ggggaattac gaccagtacc tgcaggaaaa
    12961 agaagaagcc ctgcgcgtgg aagaactaca aaatgccgag ttcgatcgca aactggcgca
    13021 ggaagaagtg tggattcgcc aggggatcaa agcacgccgt acccgtaatg aaggccgcgt
    13081 acgcgccctg aaagcaatgc gtcgcgagcg tggtgaacgt cgcgaagtga tgggtaccgc
    13141 aaagatgcag gtggaagagg ccagccgctc cggtaagatc gttttcgaaa tggaagacgt
    13201 ttgctaccag gttgacggta agcaactggt gaaagatttt tcggcccagg ttctacgtgg
    13261 cgacaaaatt gccctgattg gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat
    13321 gctcggtcag cttcaagcgg acagcgggcg tattcacgtt ggcaccaaac tggaagtggc
    13381 ttatttcgat cagcaccgcg cggaactgga tcccgataaa acggtgatgg ataaccttgc
    13441 cgaaggtaag caagaggtga tggttaacgg caagccacgc cacgtattgg gctatttgca
    13501 ggactttctg ttccatccga aacgggcgat gacgccggta cgtgcgcttt ctggcggtga
    13561 gcggaaccgc ttgctgctgg cgcgtttgtt cctcaaacca agcaacttat tgattcttga
    13621 cgaaccgacc aacgatcttg atgtcgaaac gctggaactg ctggaagaac tgatcgacag
    13681 ctatcagggc acggtattgc tggttagcca cgatcgtcag tttgtcgata acaccgttac
    13741 agaatgttgg atcttcgaag gcggcggtaa aattggtcgt tatgtcggcg gttatcatga
    13801 tgcccgtggt cagcaagagc agtatgtggc gctcaaacag cctgcggtga aaaaaaccga
    13861 agaagccgcc gcggcaaaag cagaaactgt aaaacgcagc agtagcaaac taagctataa
    13921 attgcagcgc gaactggagc agctaccgca attgctcgaa gatctggagg cgaagctgga
    13981 agccctacag acgcaggtgg cggatgcttc cttcttcagt cagccacatg agcagacaca
    14041 aaaagtgctc gccgatatgg ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg
    14101 ggagtatctt gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag
    14161 acagctgaca acggtaaaat tgcctgatga actgggttta tcaggcatga aaaccaacac
    14221 tttgttagtc atctgtaaaa tagcgcatca ttaaggagta ccaatgtgcg aacatcatca
    14281 tgccgcgaag cacatcctgt gctcgcagtg tgacatgctg gtggcgttac cgcgccttga
    14341 gcatggtcag aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc
    14401 ccctcggcag cgtcccaccg cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa
    14461 cttgtttcct tttgtgaata tgaacgttgc aggagttacc agtgaaatta cattactgga
    14521 aattcccggc gtgctttttt ctgaggacta cgccagcctc ggcacctttt tcctgttgtt
    14581 tgtgcaactg gttcccgcgt tttgtctgat aaccattctg ttactggtga atcgcgcgga
    14641 attaccggtc cgtttaaaag agcaactggc acgggtgctt tttcaactca aaacctgggg
    14701 aatggcggag attttcctcg ctggtgtgct ggtcagtttc gttaaactga tggcttacgg
    14761 cagcattggg gtaggcagca gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg
    14821 cgcttttcag tgcgttgatc gtcgctggtt atgggacgac atcgccccga tgccagaact
    14881 gcgccagccg ctaaaaccag gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg
    14941 ttgtacggca atccttcctg ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta
    15001 cgttcggcgt agaaacagcc tgcagtggac actcgcgctg cttgtaacgt ccatcatgct
    15061 gtatcttccg gctaatattt tgcccatcat ggtgacggat ttattaggct cgaagatgcc
    15121 gtcgacgatt ctcgctgggg tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc
    15181 ggtgatcttt ctggccagta ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct
    15241 gtgttgggat gccaaagggc atggcaagcg cgacagtgaa agaatgcatt tgatttatga
    15301 agttgttgag tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg cggtgctctc
    15361 ggcgctggtg cgtatgggag gtttaatgag tatttatccg gcaatgggtg cattaatgtt
    15421 tgctttagtc gtcataatga caatgttttc tgctatgacg tttgacccgc gtttgtcgtg
    15481 ggatcgtcaa cctgaatcag agcatgagga gtcctgacag tatggaatct aataatgggg
    15541 aagccaaaat ccagaaagtg aagaactggt ctcccgtgtg gatatttcct atcgtcacgg
    15601 cgctcattgg ggcctgggtt cttttttatc attacagcca tcagggaccg gaagtgaccc
    15661 tgatcaccgc gaatgcggaa ggaattgaag gtggcaaaac caccattaaa agccgtagcg
    15721 ttgacgtcgg cgtggttgaa agcgccacac tggctgatga tttgacgcac gttgaaatca
    15781 aagcgcggct gaattccggt atggaaaaat tgctgcataa agacaccgtc ttttgggtgg
    15841 tgaaaccgca gattggtcga gaagggatta gcggtctggg aaccctgctg tctggggttt
    15901 atatcgaact gcagccaggc gcgaaaggca gcaaaatgga taaatacgat ttgctggact
    15961 cgccaccgtt ggccccgcct gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag
    16021 ccgggcagct ctcgccagga gatccggtgc tgttccgtgg ctatcgggta ggttcggttg
    16081 aaaccagcac cttcgataca caaaaacgca atatcagcta tcaactgttc atcaatgcac
    16141 cttatgaccg actggtgacc aacaatgttc gcttctggaa agatagtggc attgcggttg
    16201 atctgacgtc agcagggatg cgtgtggaga tgggctcatt gacaacgctg ctgagtggcg
    16261 gtgtcagctt tgatgtgccg gaaggtctgg atttagggca gccagtggca ccgaaaacag
    16321 ctttcgtttt gtatgatgat cagaagagca ttcaggattc gttgtacacc gatcacattg
    16381 attatctgat gttctttaaa gattcggtac gcggtctgca accgggagct ccggtagagt
    16441 tccggggtat tcgcctgggt accgtaagca aagtgccatt ctttgcgccg aatatgcgtc
    16501 agacatttaa cgatgattac cgtattccgg tactgattcg tatcgagcca gagcggctga
    16561 aaatgcagct tggcgaaaat gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg
    16621 gtttacgcgg atcgctgaaa accggaaacc tggtcactgg tgcactgtat gttgatctcg
    16681 atttctatcc aaatacgcct gcaataaccg gtattcgtga atttaatggt tatcagatta
    16741 tcccgaccgt tagcggcggc ctggcgcaaa tccagcaacg actgatggaa gcgttggata
    16801 agatcaacaa actgccattg aatccgatga ttgaacaggc aaccagtacg ctttctgaaa
    16861 gtcagcgcac aatgaaaaac ctgcaaacga cgctggatag catgaacaag atcctcgcta
    16921 gccagtcgat gcagcagttg ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca
    16981 gcatgcaggg cttccagcct ggctccgcag cctacaacaa gatggtggcg gatatgcagc
    17041 gccttgatca ggtgttgcga gaactgcaac cggtgctgaa aacgctcaat gagaagagta
    17101 acgcgctggt atttgaagcg aaggacaaaa aagatccaga gccgaagagg gcgaaacaat
    17161 gaaaaagtgg ctagtgacga ttgcagcact gtggctggcc ggatgcagct ccggcgaaat
    17221 taataaaaac tattaccagt tacctgtggt gcagagcggt acacaaagta ccgccagcca
    17281 gggcaatcgt ctgttatggg tagagcaggt cactgttcct gactatctgg cggggaatgg
    17341 tgtggtttat caaaccagtg atgtgaagta tgtgattgcc aataacaact tgtgggccag
    17401 cccgttggat caacagttgc gcaacaccct ggttgccaac ctgagtacgc aactgcccgg
    17461 ctgggtggtt gcctcccagc ctctgggaag cgcccaggac acgctcaatg ttaccgtcac
    17521 ggagtttaac ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc tgttgaacca
    17581 ccagggacaa ctgatcaaac gtccgttccg tctggaaggg gtgcaaactc aggatggtta
    17641 cgatgagatg gttaaagtac tggccagtgt ctggagtcag gaagccgctt ctattgcgca
    17701 agagataaag cgtctacctt aattataaag atttgtaaat ataaccgcct ccggtatgtt
    17761 gcctgaggcg gtttttttgt ctctaacgtg cggaaaaagt tgttcctctt cacatttttt
    17821 gtacaaccgg catacccgtg tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg
    17881 acggcagtta tgattcgcgg tattgcttaa ctgtgattgc acatttagta atcactgttt
    17941 tcttttccac cagaaaccag tatgagggaa acgaggcatg aagagacaaa aacgagatcg
    18001 cctggaacgg gcacatcaac gtggttatca ggccggcatc gccggacgct caaaagaaat
    18061 gtgtccctat cagacgctga atcaaaggtc acaatggctg ggaggctggc gagaagccat
    18121 ggcggacagg gtagtaatgg cctgattctg tctctttaaa aagaaacctc cgcattgcgg
    18181 aggtttcgcc ttttgatact ctgtctgatt ataatcagaa ggcagacgta tcctggaaca
    18241 gaccgacttt caggtcgctg gcggtataga tcagacgacc atcaaccagc acttcgccat
    18301 ccgccaggcc cataatcaga cgacggttaa caatgcgttt aaagtgaata cggtaggtca
    18361 cttttttcgc tgtcggcagt acctgaccag tgaatttcac ttcgccaacg cccagcgcgc
    18421 ggcctttacc ttcgccgccc agccagccga ggtagaaccc taccagctgc cacattgcgt
    18481 ccaggcccag gcatcccggc ataaccggat cgccaataaa gtggcatccg aagaaccaca
    18541 gatccggatt gatatccagt tctgcttcaa catacccttt gtcgaagtta ccacccgttt
    18601 cggtcatttt gaccacacgg tccatcatca gcatgttcgg tgctggcaat tgcgggcctt
    18661 tagcgccaaa cagttcaccg cgaccagagg caagaaggtc ttcttttgta taggattcgc
    18721 gtttatctac catgttctct gtaagcctta ttttattgaa gcacgcagga tagctaacac
    18781 gtgtacgctg aacaagtccg atcagttcgg aataaaccag ttcagccaac gtaatggcca
    18841 tggaaaacgg tgacgtcctt cctgttgcga tgcttgcgcg atacgttcct ggatggtttg
    18901 catcagcgtc gtttggcctt cgccatccca caccagattt aataataacg gcagtgcgtc
    18961 agtcacatcg tctactgccc agatggtgaa tttgccttct tctaccgctt tcaccagttc
    19021 actgtgaaga cttaaatggc gaacgttagc tgtggggata atgacacctt gtttcccggt
    19081 taactcacgt tgctggcaaa tagcaaagaa gccttcgatt ttctcattta aaccaccgac
    19141 cggctgggcg cgaccgaact gatcgactga acctgtgata gcgatactct gattcaccgg
    19201 cacatcggcg agggcgctta tcagggcgca gagttcagcc atcgaggcac tatctccatc
    19261 aacttcactg tatgactgct caaatgtcag cgatgctgag aaggggatct gttgctcaag
    19321 ctgtagttcc gacatcagga acgcttgcat gatcatcatc cctttcgcat ggatattgcc
    19381 gccaagctcc gctttgcgtt cgatgtcggt gaattcacca tcgccaatat gcacaacgca
    19441 gctaatgcga gaaggttcgc caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag
    19501 ggcgttaatt tgcccgatgc gttcgccttc ggtttcaatc aggatttgct cctgaaggat
    19561 ctcatcctgc atacgttcag cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa
    19621 gtttagctgc tcgccggaga aggtgtcgcc atcacacagg gaggcgacct ctttacactg
    19681 gcggaggatc cactgcgggc taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc
    19741 ttcgcggata agtaccggcc aggcatccgc tcccggtgca ggtaagtgat tatgtctggc
    19801 ggtaaatgtc acccagcgac accactgggt tactgactct gcatcgacaa tctgcagagt
    19861 atcttcaaat tcgctataaa tagcctgctc tgaaagctct ggctccatct cctggaaatc
    19921 agacaatgat tcgcgttcgc ctaccagaat gactttcagc ttcaatggca tcgaaggcac
    19981 agagacgggg agagggcgcg actcatcaaa cgcaacccag tcaaaacgct cgcggttaac
    20041 gatatttttc agccgcatcc acagcagagg ttgcgccagc agtgtacgca aagagatgat
    20101 gagaataccg ccatttgcct gatgcaccag accaggctgc agggtaatgt cgccattaaa
    20161 ctggcgcagg cagccaaaga gttgctccgc ttctacccag tcggcagcga caacttgcgt
    20221 taaagtcgca aaattatcat ctgcactcac tgcgtgacgt aagcggatgg agtggccgga
    20281 aacctcatag tgaccgccca ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatt
    20341 ggcaataaga ttcagatact cagactcttc cggggccttc gccagcataa aagaggagga
    20401 tgctcgcgta tgcagcaact gctccagtgc gaattgcagt cgcggttgag tatcactgaa
    20461 taaaggatcg ttttcgtcaa tcaaatgtgg ctgagcaaat atttcctgat agctatcggt
    20521 atcaggaacc aggtcacgcc atgcaagttt cgtaatggtc aaagttgatg ttttttagtc
    20581 tgttgtcaaa gccgcgatta taccgtaacc ggcactacag cacacgtaga aagcaccgac
    20641 aatactcctg gcatgggcgt taaagctcac aggatggaga ttcttttctt cactggccta
    20701 aaaagctgat attctgtaaa gagttacacg gtaacattga gatcgctatg aaatatcaac
    20761 aacttgaaaa tcttgaaagc ggttggaaat ggaagtatct ggtgaagaag catcgcgaag
    20821 gggagttaat cacccgttac atagaagcca gtgccgccca ggaagccgtc gatgtgttgc
    20881 tctcgctcga aaatgaaccc gtgctggtaa atggctggat tgacaagcat atgaatccgg
    20941 aactggtcaa tcgcatgaag caaaccattc gggcaagacg aaagcgccat tttaatgcgg
    21001 aacaccagca tacgcgcaaa aaatcgatcg atctggaatt tattgtctgg caacgtctgg
    21061 ctggtcttgc gcagcgtcgc ggtaaaacgc tttctgaaac gattgttcag ctgattgaag
    21121 atgcggaaaa caaagagaaa tacgcgaata aaatgtcttc tctgaagcag gatctgcagg
    21181 cattgctggg taaggaataa ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac
    21241 cccgcagcag cggggttttt ctaccagacg agaacttaag cttgcggctg agttacaacg
    21301 tctttgatac ctttaacttc gatctctacg cgacgatccg gagccaggca gtcgatcagt
    21361 gcagcacgct gtttcacgtt gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata
    21421 ccacgtgcgg agatcttgtc tgccgggata cctttggaga tcaggtaatc aacaacagac
    21481 tgagcacggc gctcggacag accctggttg taagcgtcag aaccgatgcg gtcggtgtaa
    21541 cccagaacaa ctacggaacc gtctttcgga tccaggttgc tcagctggct gtacagctga
    21601 tccagagcag cctgaccttc cggtttcagg gttgctttgt tgaagttgaa cagaacgtca
    21661 gacttcagag tgaagtgctt ggtctgtact tccggtgccg gagctggagc cggagcaact
    21721 actggagctg cttcgccctg accgaaacgg taggaaacac ccaggctcag catgccgttg
    21781 tccggacgag tgccgatggt gtgtgcgtca ccgatgttgt tggtccactg gtattccaga
    21841 cgggtagcga tttcaggagt gatcgcgtac tcaacaccgc cagcgaagac cggagaaacg
    21901 ccggtgtcgt ggtttttacc ataaacgttg gatttagtgt ctgcacgcca taccatacca
    21961 cccagacgag tgtagatgtc caggtcgtca gtgattgggt aacccagttt agcggtcagt
    22021 tgaacgccct gagctttgta tgcaccgttt tcaacgctgc ctttgtacgg catacgacct
    22081 aaccagtcgt aacccatttc aaagccaaca tacgggttaa cctggtaacc accaaaagca
    22141 ccagcgccca gttggttttc atgggtcggg ccattgttgt tgatgaaacc agtgtcatgg
    22201 tactgggacc agcccagttt agcaccagtg taccaggtgt tatctttcgg agcggcctgc
    22261 gctacggtag cgaaaccagc cagtgccact gcaatcgcga tagctgtctt tttcattttt
    22321 tgcgcctcgt tatcatccaa aatacgccat gaatatctcc aacgagataa cacggttaaa
    22381 tccttcaccg ggggatctgc tcaatattaa ctctaccgat atcttcggct tatgccgagc
    22441 acccctggcg atgtaaagtc tacaacgtag ttgaaaactt acaagtgtga actccgtcag
    22501 gcatatgaaa aaaaagtctt gtataaggta tgtttaatct tttttgtcag cgacaattta
    22561 cagaagaaaa tcgcggaaac cgcttcagac aagcctccgc aaggaaaatt agtcacgact
    22621 gaaagcattg gctgggcgac aaaaaaagtt ccaggattaa tcctaaattt acttaatgat
    22681 acaaattaga gtgaattttt agcccggaaa gttgtctcgt ggcgtgagag gatgcgctta
    22741 ccggacgcat aataaacccc atagcgttac cttcatttgc cgcatcaaca agttcagcat
    22801 gctcttcttc agtcaaatca tctgccaacc aaccgatcac cacactgtaa ttgcccgtgc
    22861 gtaaagcgcg aaccattgac tccacagtgt ggcaagggga gagctggcta atctgcatta
    22921 ctttcgttaa gggtagccca gatgcctgaa cccattcccg actcagtttt tgttgcggtg
    22981 ttaaccagag ttgccagcgc gattgctgac cgagttgctg taacaatggc aacagtagaa
    23041 gttgcgtcat catgggctga tcttcgcgat agacaacttc actgataagc ccggctgtag
    23101 tgttttccgt agagacacgc gcaattttac ttgctgcgga tgagaacgac gaagaacgat
    23161 gtgcatagcc tgaagtgtac ataatcaatc cagcccctgt gagttactgt atggatgtac
    23221 agtacatcca gtgacaacaa agatcaaccc tattttcgga aagagcctcg caaattttgt
    23281 cgttggtgac gggaaaacat aaattaatct tgccccttaa gaataagttg cctattttcg
    23341 tagttaacgg atccgttaat gtgaatcatt cttttatgtt atgattttaa aaggaatttt
    23401 atgaaaagcc tctcctataa gcggatctat taatcacaag aatacctggc aacgttgggc
    23461 acaattgaat accgatcatt gtttggcagt tacagcctga ccgttgacga cacggtgttt
    23521 gcgatggttt ctgatggtga gttgtatctt cgggcttgtg agcaaagtgc acagtactgt
    23581 gtaaaacatc cgcctgtctg gctgacatat aaaaagtgtg gccgatccgt taccctcaat
    23641 tactatcggg ttgatgaaag tctatggcga aatcaactga agctggtgcg tctgtcgaaa
    23701 tattctctcg atgcagcgct gaaagagaaa agcacgcgca atacccggga aagactgaaa
    23761 gatttgccca atatgtcttt tcatctggaa gcgattctcg gggaggtggg gattaaggat
    23821 gtacgggcgt tacgtatact tggggcaaaa atgtgttggt tgcgactgcg gcagcaaaac
    23881 agtctggtga cagaaaagat tctgtttatg cttgaaggtg ccattatcgg cattcatgaa
    23941 gctgcgctcc cggtggcacg ccgccaggag cttgcagaat gggctgactc tcttacgccg
    24001 aaacaggagt ttcctgcgga acttgagtaa tctggcgttg cagacgacca atctcaggca
    24061 gtaatgcaat caataatcca acttgttgta cgaccagagg ttctttgctg tctgcccgtg
    24121 gttcaagttg ctgcatccgc tgtttcaggc tagctaatgc ctcattgacg cgttcttcat
    24181 cagcaggttg atgatgtaac gcgtcatcaa catagcaaac tgcgtcatca agaaacgcca
    24241 gaatttcagg attagttaac tgctcccggt gagcaccgag ggctgagata tagctggtaa
    24301 acgtatggtt aaggcacagc aaccgaaacg cggcttcgcg aatttgcggg gtaacgttcg
    24361 gctcgctgga catatttgat accaccgacg ccagctcagc atcacggttg tgtgcatcgc
    24421 ggcgggcaat acgatacgcc agacggttat cacgcccctg atggtattgc tccagtatgg
    24481 catcgagata ccgacagttg gcctctgtgg cgcgttcgag catgcgcggc agattgcgaa
    24541 actgccagtc aggccagatg tagctcactg ccgcccacgc aatggcacaa ccaatcagcg
    24601 tatcgattac gcgaggtaac gctacttcaa aaccttcacc cagtaagtta aaacacagta
    24661 gcaccaaaag tgtgatgaac atcgttgcat gagcgtattg cacgttacgg aaggcaaaaa
    24721 agagcacgcc ggtaataacc agcagcacca gctgcccttc cagtgatggc acaaaccaca
    24781 gcacaggaat gccaatggcg atacctacca gcgtaccaat aatccttaac ttcaggcggt
    24841 ggcgcgtggc gttatagttt ggctggcaga caaacaaact tgtcagcaag atccaatacc
    24901 cgtgatgcat tccggttatt tgaatgatgg cgtagccgaa gcacaacacc agcgacattc
    24961 ttaccgcatg acggaagagg gcggattccg gcgtgaagtg acggctaaga cgcagccaga
    25021 tatcactcaa cccgtgcggg ctgtcatcag cgagctcatt ttcgtcatta ttatggggta
    25081 gtgcctgggc ctgttctgat tcaattgttg ccagttgggc atcaatggcg cgtaaattgt
    25141 tcagcaaaaa tcccagtgtt ttgagtaaat cggcgggtgc gccgttatcg cgcatccgct
    25201 ccagcgcagc atcaatatgc gtaaaagcgc gctcaaaatg cggatcatgt tgataaggct
    25261 gacgcaacaa aatacagcgt gacagttgct ggcacgcctg gccctgcatc gacatcagcc
    25321 gctgaaaacg gaacagcacg tcgctgtggc gaaaatgttc acgcaatgtt tgatactgaa
    25381 tatgagaaga gctggcacgc tcgtgaatat cctgtgcgac aaagtaataa tgcagcgtgc
    25441 gacgcgttcc ccgttgacca cgatcgccac gtaagcgggt cagcagcgag agtttcgtct
    25501 gattcaatgt cgccatcagc agaccgttgg cgagagccaa atcgtacagc ggtgcctggc
    25561 tttcatcttc aatatcagga tcaaacatgc gcgacttgag ctcaagataa cgcgccagtt
    25621 gttcatagca acgcgccagg ttgtcctgca gcgggcggac cgggaacagc agatgaccaa
    25681 taagtgtcag gacgttgtac cagacggcac cggccagcag atacatcggc tgctgatacc
    25741 agtgctcata cagtgatgtt cccaacatag tgtaaatggc gatcagcaat gcaccgaagg
    25801 caattgttgc atagcgttga cccagaccgc cgagcaaaat gaagccgctg gtagagagcg
    25861 ttaagccaat cgcaaatagc cagggccagg gaaacagcaa ttctactgag gccgaggcga
    25921 taaaaaagca gaacagcgta atgatgaggt tacgcaaacg tcccgccagt cggtcatcga
    25981 gatcggtcag cgccgctgcc accatcccca gcgttagcgg aatcgtcagt tttacatcac
    26041 ccagccacca cggaaacgct gtggttccac aaagcgcaat aaaaatacgc gcgtaataca
    26101 gccaggcgct gttccaggta tagcgtttga gcaaaggact tagcataaag gccatattag
    26161 tggattattc aaaacgacga cgtgcattgg cttcacgcgc agcttgcgct gtttctaccg
    26221 ataccacgcg acgaccaacc ggccatagtg caatagcggc aattttaaag ttcgcaatgc
    26281 cgacaggaat gccaatgatt gaaatacatt gtgcgatgcc cgttgcaatg tgcatcaggc
    26341 ataaccacca gccaaagaaa atcagccaga aaatattcaa taccgtaccg ccagtattca
    26401 gcagtacatt tttgccagcc gggttcagtt catcgacatg aatagcttca ttgccataag
    26461 gcaccagaga cagtttagtg atctcccagc aggatcgtgt cagcggtaag gtaaaaatca
    26521 gcacaatact gaccagagtc gccaacagcc agcccagagt ggtggcaaat ccgccaagca
    26581 caaagttcag aatgttcaaa acggtacgca taaaacctcg ctttactgtg gttttcagta
    26641 atgggcggca attgtaacgt ttttttgggc tggagcacgt tttctctgac ggttacactg
    26701 ataagaaata atttcgtgtg gatctacaga gtcatggaac tgaaagcgac aacgcttgga
    26761 aaacgtctgg cacagcaccc ttacgatcgg gcggtgatcc tcaatgccgg gattaaagtc
    26821 tccggcgatc gccacgaata ccttattcct ttcaatcaat tactggcgat tcactgtaag
    26881 cgcggtctgg tatggggcga gctggaattt gtactgccgg acgaaaaagt ggtgcgtctg
    26941 cacggcaccg aatggggcga gacgcagcgt ttttaccatc atcttgatgc tcactggcgg
    27001 cggtggagtg gcgagatgag cgaaattgcg tctggtgttt tacgccagca actggatttg
    27061 attgccacgc gcactgggga aaataaatgg ctgacgcgtg agcaaacctc tggcgtgcag
    27121 caacaaatcc gccaggcttt gtcggcgttg ccgttgccgg ttaaccgact ggaagaattc
    27181 gataactgcc gtgaggcgtg gcgtaaatgt caggcctggt tgaaagatat tgaaagcgct
    27241 cggttgcagc ataaccaggc gtataccgaa gccatgctta ccgagtatgc ggattttttc
    27301 cgccaggtcg agtcttcacc gctgaatccg gcgcaggccc gggcagtcgt taatggcgag
    27361 cattctctgt tagtgctggc aggtgcagga agcggaaaaa cgtcggtgct ggtggcccgt
    27421 gcaggctggt tgctggcgcg tggtgaagcg tcccctgagc aaattttatt gctggcgttt
    27481 ggtcgcaaag ccgctgaaga gatggacgag cggattcgcg aacggctaca taccgaagac
    27541 attactgcac gcacgtttca tgcgctggcg ctgcatatta ttcagcaggg cagcaaaaaa
    27601 gttccgatag tcagcaaact ggaaaatgat accgctgccc gtcatgaact ctttattgct
    27661 gagtggcgca agcaatgcag cgaaaagaaa gcgcaggcga agggctggcg gcaatggctg
    27721 acggaagaaa tgcagtggtc agtgccagaa ggtaacttct gggatgatga aaaattacag
    27781 cgtcgccttg cctctcgcct cgatcgctgg gtaagtctga tgcggatgca cggtggtgca
    27841 caggcagaaa tgattgccag tgcacccgaa gagattcgcg atctgttcag taaacgtatc
    27901 aagttgatgg ccccgttatt aaaagcctgg aaaggtgcgc tgaaggcaga aaacgctgtc
    27961 gatttttcgg gccttattca tcaggcgatt gtgattctgg agaaaggtcg ctttatcagc
    28021 ccgtggaagc atattctggt tgatgaattt caggatatct cgccgcagcg ggcagcgttg
    28081 ttagcggcat tacgcaagca aaacagtcag acgacgttgt tcgccgttgg tgatgactgg
    28141 caggcgattt accgattcag cggtgcgcaa atgtcgctca ccaccgcttt ccatgaaaac
    28201 tttggtgaag gcgatcgctg tgatttagac acgacttacc gttttaacag tcgtatcggt
    28261 gaggtggcaa accggtttat tcagcagaac ccaggccagt tgaaaaagcc gctaaacagc
    28321 ttaaccaatg gagacgaaaa agccgtcacg ttattggatg agagtcaact tgacgctttg
    28381 ctggataagc tctctggtta tgccaaaccg gaagagcgca ttcttatcct ggcgcgttac
    28441 catcacatga ggcctgccag cctggaaaaa gcggcaacac gctggccgaa gttgcaaatc
    28501 gactttatga ccattcatgc cagcaaaggg caacaggcgg attacgtcat catcgttggc
    28561 ttgcaggagg ggagtgatgg ttttccggct gcggcgcggg agtcgattat ggaagaggcg
    28621 ctactgccac cggttgagga tttcccggac gctgaagaac ggcggttaat gtacgtggcg
    28681 ctgacccggg cacgccatcg ggtatgggca ctgtttaaca aagagaatcc ctctcccttt
    28741 gtggaaatac tgaaaaatct ggatgtgccg gtggcgagaa aaccgtaaga aacaggtggc
    28801 gtttgccacc tgtgcaatat tacttcagac ggtccgcgag ataacgctga taatcgggga
    28861 tcagaatatc gaccgcgtcg ttgaaatgcg gcgactggat aatgaagtct gccgttgcca
    28921 cgttggtggc gaccggaatg ttccataccg tcgccagacg cagcaaggct ttcacgtcag
    28981 gatcgtgcgg cacggcattt agtggatccc agaagaaaat caatacatca attttccctt
    29041 ctgagatcaa tgcgccaacc tgctggtcac cccccattgg gccactcaac atcgcgttga
    29101 cgttcatgcc ggttgcacgg gaaatcaagt taccggtagt gcctgttgca tacagtacgt
    29161 gttgttccag taacggttga tgccgttcca cccagctcat cagcatttgt ttgcagtgat
    29221 cgtgtgccac cagcgcaata tgtttccgcg caggtaaagt gcgagtcgtc agttccataa
    29281 tgtacatccg tagttaactt tcctacagat tactgtaagc acttatcgct gcaagataaa
    29341 gaccgaaaaa gcctgcgcac aggcacaaaa atctcaggaa gatggttgtt tttccgccca
    29401 ctgcaggaaa gtatttcgcg tttgtgggtc agccagttta aaccaatact tcagccgttg
    29461 ttctgtgagc acctgagact gcggtggaat agcatccagc tcggaaacac ccgaaagcag
    29521 cgtactgtca tctgccatca tcgtggcaaa ttgcggtagt gaagcgcgtt tcgcggattt
    29581 gttatagcgt tcaacctcga cctcataatc aatcgttttt gccgttgagg taatggcgag
    29641 aatatccagc tttaccggaa tcggcgtcgc atcgccatcc aacaattcaa ggcgcggcgc
    29701 ggcatcaaaa tggttagctt cccgctcatt ctccaggcga ggcaggcgaa aattgacctg
    29761 gttgatgagc tgggtattaa aactgaccac cagcggtggg gagatataca gccgttcttc
    29821 actattggaa agatgaatcg tcttctcaac gcgaaacact aactgatgcg gtccattatc
    29881 cagttcaatg ctgtccgcac cgcgcagcag agagctggaa accttcttgc cgtcgagtac
    29941 cagaagatcg acatcggttg aaagccgcag cgtggtggca aaaacggata ccggcaaaca
    30001 taatgcaatc aaggtggtca cgatgccggt tttcataggt agctcctgtc aaaagaccgc
    30061 ccgaataata atgtgtcaaa atttttcttt aatcatactt actaacatat agacatattt
    30121 cccgcttttg ctctcattca ttcgtattag ctgcatggtt ggcatgtcgg ctttgtcgta
    30181 cactttgtta aacagtctgg aggaaataat gaaagaaacc gatattgcag gcattttaac
    30241 gtccactcac accattgcgc tggtgggggc aagcgacaaa cccgatcgtc ccagctatcg
    30301 cgtgatgaaa tatctactcg accaggggta tcacgtcatt ccggtttcgc caaaagttgc
    30361 cggcaaaacg ctgctgggcc agcaggggta tggcacgctg gcagacgttc cggaaaaggt
    30421 cgatatggtc gatgttttcc gtaattcaga agcggcgtgg ggcgtggcac aggaagctat
    30481 tgccattggc gcgaaaaccc tctggatgca actgggggtg attaatgagc aagcggcagt
    30541 actggcacgg gatgccgggt tgaatgtggt gatggatcgc tgcccggcta ttgagatccc
    30601 tcgcctgggc ctggccaaat aaaaaatccc cggaaggcaa aaaccttccg gggatacgtt
    30661 cagggatcag ttacgcagac gcggggcctg gagttgtttg cggatggtct gcgccagctc
    30721 atccatggat ggctgttcag gatgttcatc ctgcaattcg ctacttagct gggcttcagc
    30781 caggtaggta tgaaccggta ggccgttatc gtcctccatc accacgtgat accacggagc
    30841 ggcgcgaagc tcgtcattca ccgccagctc atcaggcgac ggttcagaga gcgaataaac
    30901 cgggtcgata tccacgacca ctccgagata acctaacagg gaatggcgga cctgctggcc
    30961 gataccgaat ttgctggcaa tcatagtcac ctcccgggaa atctttacct ttgatataag
    31021 ggtaaaattc cacatttcaa gttacatgac gcgacaggca aaccctttca gatatagccc
    31081 ttccggatag gtagcgatca ccggatgatc ggctgcctga cggaactgct ctataaattg
    31141 tacatcacgg ccggcatcaa ttgcggcatc cgcgatgatt ttctgaaata aatcgctggt
    31201 catcagaccg gaacaggaga aagtcaggag aataccgcct tcattcagca actgaatcgc
    31261 cagcatgttg atatctttat agccacgaca cgcgcccatc aactggcttt tattctcaac
    31321 aaacttcggc gggtccatca cgataacgtc aaatttttca ccgcgatcgc gataagtacg
    31381 cagcaattta aagacatcat cacggacaaa ctcagccttg ctcagatcca gtttgttcag
    31441 ctcaacgttc tgccgtgcaa tatccagcgc ttcctgggag gtatcaacgc tgacaacctg
    31501 gctgcaaccg cccatcagtg ccgatacggc gaaaccaccg gtataggaga aacagttcag
    31561 cacacgttta ttttcaacgt agcggcgggt agccaggcgg ctatcacgct ggtccaggta
    31621 gtagcccgtt ttgtgtccgt gctgaatatc caccagcagc ttcatgccgt gttcttcaat
    31681 cggcagcagg gcaggcggca actcgccggt gacgaggccc tgggtcagct ccatcccttc
    31741 ttttttacgt accgcaacat cgctgcgatc gtaaatcgca cattccgggt acagcgtttg
    31801 cagggcacta actaatgccg cgcgctgata ttctgcccca gcactcagca gttgcagcac
    31861 cagaaaatta ccgaaacgat cgatagtaat acccggcagg ccatcagatt ctccggcgat
    31921 taaacgatag ctgtcgaggc catctttttg cgccagccag tcacgccatt tttgtgcttg
    31981 ttgcaaacgg cgggaaaaaa aagcaatgtc gatagactca gacgggtcaa acgtccagac
    32041 gcgcgcccgg atttgcgaag ctggtgaata agcgccgcgt gctaaccatt ttccctgatg
    32101 atcaacaata tcgatggttt caccgaggct ggctttacct tccatgcggg caacggcccc
    32161 ggaaaagacc cacggatggc gacgaagtaa tgatttttcg cgccctttgg ctaacactaa
    32221 acgtacactc ataatttact tttctgtcga tgcaaagaaa tgggcgtcat tgtccggagt
    32281 tttccatgga tttgcaacgc gtccattaag gataaggaga gcagcaatgt cgaaagtctg
    32341 cataattgcc tgggtttacg ggcgagttca gggcgtagga tttcgctaca ccacacagta
    32401 cgaagcgaaa agactgggtt taaccgggta cgccaaaaat cttgacgatg gcagcgtgga
    32461 agtggttgcc tgcggtgaag aagggcaggt ggaaaaatta atgctgtggt taaagagtgg
    32521 cggcccgcgt tctgcgcgtg tagaacgggt gctcagcgag ccgcatcatc cctcggggga
    32581 attaaccgat tttcgtatcc gttaaataca ttttaccggt tttggcaggc cagcaatttt
    32641 ggtggcttgc ttagccggac ctttcggaaa cagtcggtat aaatagcggc tgttaccttt
    32701 ttcttcgcca aatttattcg ccatcgcttt taccagcata cgaatcgccg gagaagtatt
    32761 gaattccaga tagaaatcac gcacaaaacg caccacttcc cagtgttccg gtgagagcga
    32821 aatcccttcg ttctctgcaa tcaccactgc cagcggctca ctccactggc tgctttcttt
    32881 gagatagcct tcggtatccg tttctatctc tttaccttcg aagatcagca taattattac
    32941 taccttaatc agactggcgg cagtgtaaca aaaaacaaag ccccgcataa agcgaggcta
    33001 tgaaagtgtt agcgggtgag attaatcgcg gctggcgaag cccagaatgc tcagcaggct
    33061 gacgaagatg ttgtacagcg aaacatacag gctaaccgtg gcacgaatat agttcgtttc
    33121 accgccatga acgatgttgc tggtttcaaa caaaatagcg ccagaggaga tcaaaatgaa
    33181 gaccgcgctg atcgccagat gcagagcagg cagctgcagg aagatattag caaccatacc
    33241 aatcagcacc accacaatac ccgccatcag cataccgccg aggaacgaca tatctttgcg
    33301 ggtggtcagc acatatgcag agcagcagaa gaacactaac gccgttccgc ccagtgccat
    33361 agcgattacg tcacccattc cggcagacag ataggtgttc agaataggcc cgaggatata
    33421 acccagaaaa ccggtaaagg cgaatgcgga gataatcccg gtcggcttat tcgccgtttt
    33481 ataggtcagg aacatcaaac catacatacc caccagcgtc agaatcagac ccggagatgg
    33541 cagcatcagc accgtactgg cagttgcggt aattgccgaa aaggccagcg tcaggctcag
    33601 cagaaaatag gtattacgca gcaccttatg ggtgctaagc agtgatgtac ggtcatgtga
    33661 agaactaaca atacgatcca ttgagtcact ctctatgaca gatgtaatta attaagcagc
    33721 ataatgataa tgcgtaaggg cacccagaag ttttacccat ctttacgcat ttgatctgga
    33781 acaggtttaa cagcggatta tcaggtcatt aagcaaatat aacgccctga gaatttcgac
    33841 aggcaaaaga aaaaggggtt agcatttagc taacccctta tcttatttgg cggaagcgca
    33901 gagattcgaa ctctggaacc ctttcgggtc gccggttttc aagaccgaag aaaaacaaat
    33961 aaaaatcagt gcaatacgtg aaattgctga aaagttaatc aaatttaaca ccaagaaaaa
    34021 tcaaggtatt aggaagttgc tcatagctag tataccaagt ctttttgcca gaaatttttc
    34081 tggattgtct acttgctaac cacgagcggc aaatccagtg ttggtgtgat ttttgtttta
    34141 cgatcgtaaa tcaacacctg attttctgtt ttatgcccgc tgaaaatttg tttgtcgcga
    34201 ctgctgcctt cgtaatctga gatccccttg gcttttatgt cgtgaaaatt gcacccgaac
    34261 ggaacgccaa ctttttgctc ggctgcgcgt ttagcctgat tccaccagtt attcagcgtt
    34321 ttagctatga ctttcccgcc tttggttgta ttgatcacat attcgcatgt acaggaagat
    34381 acatttcggg ctaactggat cgctgtacgt aatcgcggtg accattcctt gatttgtttg
    34441 gtgccggttt tgttttgctc aatgtccctt tatccataat atcctgccat ttcaactcaa
    34501 gcacatcacc gagtcttgcc gcacagagat aggaaatctc cattgcaatg cgtaactgtg
    34561 gaattgcttc cgcatatatc gccgcatact gttcatcggt gatgtaaaca gtgcgggctt
    34621 taagagagaa ttttctgact cctttgcatg ggttattctt cacataccca cgctcatatc
    34681 cccacccgta tactcgactc aggcttgcca gttcatgatt tgcctgggtc ttgctctcaa
    34741 gccctcgttt atccatgaaa attcttacct gctcagtttt gacattatca gcaagcactt
    34801 ttccgaatac catcagcaac gccttctgat gttgtcgata atctttttgg gttcgggggg
    34861 ccagttctgt aaatgcaggg gagtccataa acatgtgcca taatttagct acggtcatta
    34921 tgttgtggag ttttgctttt tccagttcat aattttgcca gactttagct acgctggttt
    34981 tccgcactct tcctagccct atagttcttg tacttccttc tggtttccac acgtaactgt
    35041 aaccattcga tctaacccgc ggtggcagta cattatcttt tttatttttt cttggtcttc
    35101 ccattattca gcgcctcaaa atcgggttca gcagaaacca gttcagatgt ttttggcatc
    35161 gttgtcagtc cgtgtggaat atccctgcgt agaactattg gttcgttttt aggaccgatt
    35221 acaaatggga tgccgtgcag tcttaactgg tgttgctgtt ttgtgtatcg ctcgtatttc
    35281 gtgatctctt taatctctgc tggagataga gttaattcgt acatgtggtc acgtgccttt
    35341 acagcatgac cgccgccact gtagctggtg ggcggcgata tgggttgaac attaaaaatc
    35401 agcctgactc gggatcagtt tttgccagat agctgaaacg tattttgcct gataacgggc
    35461 gtcatcaagt gcattatggc gctcaccttc gaatgggata gc
//
LOCUS       DAZGTR010000036        35065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGTR010000036 DAZGTR010000000
VERSION     DAZGTR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.036"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            181..765
                     /gene="yadN"
                     /locus_tag="ACHLCY_001982"
     CDS             181..765
                     /gene="yadN"
                     /locus_tag="ACHLCY_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0369245.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            870..1610
                     /gene="yadV"
                     /locus_tag="ACHLCY_001983"
     CDS             870..1610
                     /gene="yadV"
                     /locus_tag="ACHLCY_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0369246.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1645..4245
                     /gene="htrE"
                     /locus_tag="ACHLCY_001984"
     CDS             1645..4245
                     /gene="htrE"
                     /locus_tag="ACHLCY_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFP0369247.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVIQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESNPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4262..4831
                     /gene="yadM"
                     /locus_tag="ACHLCY_001985"
     CDS             4262..4831
                     /gene="yadM"
                     /locus_tag="ACHLCY_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0369248.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4846..5448
                     /gene="yadL"
                     /locus_tag="ACHLCY_001986"
     CDS             4846..5448
                     /gene="yadL"
                     /locus_tag="ACHLCY_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP0369249.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5475..6071
                     /locus_tag="ACHLCY_001987"
     CDS             5475..6071
                     /locus_tag="ACHLCY_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0369250.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6055..7398
                     /locus_tag="ACHLCY_001988"
                     /pseudo
     CDS             6055..7398
                     /locus_tag="ACHLCY_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            7511..8305
                     /gene="panB"
                     /locus_tag="ACHLCY_001989"
     CDS             7511..8305
                     /gene="panB"
                     /locus_tag="ACHLCY_001989"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP0369251.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8317..9168
                     /gene="panC"
                     /locus_tag="ACHLCY_001990"
     CDS             8317..9168
                     /gene="panC"
                     /locus_tag="ACHLCY_001990"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP0369252.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPSLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9242..10144)
                     /gene="rpnC"
                     /locus_tag="ACHLCY_001991"
     CDS             complement(9242..10144)
                     /gene="rpnC"
                     /locus_tag="ACHLCY_001991"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP0369253.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            10418..10798
                     /gene="panD"
                     /locus_tag="ACHLCY_001992"
     CDS             10418..10798
                     /gene="panD"
                     /locus_tag="ACHLCY_001992"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP0369254.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10802..12031)
                     /gene="yadE"
                     /locus_tag="ACHLCY_001993"
     CDS             complement(10802..12031)
                     /gene="yadE"
                     /locus_tag="ACHLCY_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0369255.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12095..12535)
                     /gene="yadI"
                     /locus_tag="ACHLCY_001994"
     CDS             complement(12095..12535)
                     /gene="yadI"
                     /locus_tag="ACHLCY_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0369256.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDLAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12640..13410)
                     /gene="yadH"
                     /locus_tag="ACHLCY_001995"
     CDS             complement(12640..13410)
                     /gene="yadH"
                     /locus_tag="ACHLCY_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0369257.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13407..14333)
                     /gene="yadG"
                     /locus_tag="ACHLCY_001996"
     CDS             complement(13407..14333)
                     /gene="yadG"
                     /locus_tag="ACHLCY_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0369258.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14442..15104
                     /gene="can"
                     /locus_tag="ACHLCY_001997"
     CDS             14442..15104
                     /gene="can"
                     /locus_tag="ACHLCY_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP0369259.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15145..15681)
                     /gene="hpt"
                     /locus_tag="ACHLCY_001998"
     CDS             complement(15145..15681)
                     /gene="hpt"
                     /locus_tag="ACHLCY_001998"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP0369260.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15887..18277
                     /gene="gcd"
                     /locus_tag="ACHLCY_001999"
     CDS             15887..18277
                     /gene="gcd"
                     /locus_tag="ACHLCY_001999"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP0369261.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18324..19874)
                     /gene="cueO"
                     /locus_tag="ACHLCY_002000"
     CDS             complement(18324..19874)
                     /gene="cueO"
                     /locus_tag="ACHLCY_002000"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP0369262.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20040..20387
                     /gene="yacC"
                     /locus_tag="ACHLCY_002001"
     CDS             20040..20387
                     /gene="yacC"
                     /locus_tag="ACHLCY_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP0369263.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20493..21359
                     /gene="speE"
                     /locus_tag="ACHLCY_002002"
     CDS             20493..21359
                     /gene="speE"
                     /locus_tag="ACHLCY_002002"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP0369264.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21375..22169
                     /gene="speD"
                     /locus_tag="ACHLCY_002003"
     CDS             21375..22169
                     /gene="speD"
                     /locus_tag="ACHLCY_002003"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP0369265.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22207..22569)
                     /gene="yacL"
                     /locus_tag="ACHLCY_002004"
     CDS             complement(22207..22569)
                     /gene="yacL"
                     /locus_tag="ACHLCY_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP0369266.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22744..25341)
                     /gene="acnB"
                     /locus_tag="ACHLCY_002005"
     CDS             complement(22744..25341)
                     /gene="acnB"
                     /locus_tag="ACHLCY_002005"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP0369267.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25696..27549
                     /gene="yacH"
                     /locus_tag="ACHLCY_002006"
     CDS             25696..27549
                     /gene="yacH"
                     /locus_tag="ACHLCY_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP0369268.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESCPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(27620..29044)
                     /gene="lpdA"
                     /locus_tag="ACHLCY_002007"
     CDS             complement(27620..29044)
                     /gene="lpdA"
                     /locus_tag="ACHLCY_002007"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP0369269.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29369..31261)
                     /gene="aceF"
                     /locus_tag="ACHLCY_002008"
     CDS             complement(29369..31261)
                     /gene="aceF"
                     /locus_tag="ACHLCY_002008"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP0369270.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGVVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDK
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31276..33939)
                     /gene="aceE"
                     /locus_tag="ACHLCY_002009"
     CDS             complement(31276..33939)
                     /gene="aceE"
                     /locus_tag="ACHLCY_002009"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP0369271.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34100..34864)
                     /gene="pdhR"
                     /locus_tag="ACHLCY_002010"
     CDS             complement(34100..34864)
                     /gene="pdhR"
                     /locus_tag="ACHLCY_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP0369272.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8818 a         9080 c         8423 g         8744 t
ORIGIN      
        1 tttaatttgt tcaaaagccc ttaaagtgca caataggcgt cctaatgaac aatggttgta
       61 aattttggta acaaatatca ttgctcatta atatctagca tctgcttata acatttgtgt
      121 catgaatagt cattcttcaa accaatagtc tgactaatgg aattttattc aaggattttt
      181 atgtctaaaa aattaggttt tgccctgtct ggtcttatgc tggcaatggt tgcaggtact
      241 gcttctgctg atatggatgg tggtcagtta aatatcagtg gtctggttgt tgataacacc
      301 tgtgaaaccc gcgttgacgg cggtaataaa gatggtctga tcctgctgca aactgcaact
      361 gttggtgaaa tcgatgctgg cgtgctgaat gacaccgttg gcgctaaagc caaacctttc
      421 agcatcaccg ttgactgcag caaagcaaat ccgaatccag gtagtacagc taaaatgacc
      481 ttcggttctg ttttctttgg taatagcaaa ggaacgctga acaacgacat gtctattaac
      541 aacccgtctg acggagtgaa tatcgctctg cacaatattg atggttccac tatcaaacag
      601 gttcaaatta acaaccctgg cgatgtgtat actaaagccc tggatgcaac gacaaaatct
      661 gctgtttatg attttaaagc gtcttacgtt cgtgccgtcg cagaccaaac agcaactgct
      721 ggttatgtaa aaactaacac tgcatacacc attacttatc agtaagtatt caccaattaa
      781 attgattgaa tgaatataca gggaatagtg ttaataattt ctatttttat attattccct
      841 gttttaatta actctgtcag ggatggttta tgttttttaa caccaaacat acaacagctt
      901 tatgcttcgt aacctgtatg gcttttagtt catcgtctat tgcggacatt gtcatttcgg
      961 gtactcgcgt aatatataaa agcgatcaaa aaagtgtcaa cgtacgtctg gaaaataaag
     1021 ggaataaccc gttgcttgtc cagagttggt tagatactgg cgatgacaac gctgagcctg
     1081 gcagtattac agtccctttt actgccacgc cgccagtatc gcgtattgat gccaaacgtg
     1141 ggcaaacaat caaattaatg tacacagcca gcacctcact gcctaaagac agagagagcg
     1201 tgttctggtt taacgtactg gaagttccac caaaaccaga tgcagaaaag gtcgcgaatc
     1261 aaagtctgct gcaactggca tttcgcacac gtataaaact tttctatcgc ccggatggat
     1321 tgaagggaaa tccctctgaa gccccgttag ccctgaagtg gttctggtca ggttcagaag
     1381 gtaaggcgtc attgcgagtg accaatccaa ccccttacta cgtctctttt agcagtggtg
     1441 atttagaagc tagcggtaaa cgctatccga ttgatgtgaa aatgattgca ccatttagtg
     1501 atgaggtcat gaaagtcact ggccttaatg gcaaagcaag ttctgcaaaa gtacattttt
     1561 acgccattaa tgattttggt ggcgccattg aaggtaatgc cagtctgtaa tcaggcaagg
     1621 atataattcc gcaggaagca taacgtgact ataaaaccaa ctaaaaatta tcataatcat
     1681 ctgacccgta tcgctacgtt ttgcgcgctg ctgtattgca atagtgcttt ctgtgctgaa
     1741 cttgttgaat atgaccatac cttcctgatg gggcagaatg cctcaaatat tgatctcagc
     1801 cggtatagtg aaggtaaccc tgctataccg ggtatgtacg acgtcagtgt ttatgtcaac
     1861 gatcaaccta ttattaacca aagcattaca tttattgaaa ttgaaggaaa aaaaaacgca
     1921 caagcctgta tcacacttaa gaatttattg cagtttcata taaattctcc cgatataaat
     1981 aacgaaaaag ccgttctgct tgccagggat gaaacactcg gcaattgcct taatttgacg
     2041 gaaattatcc ctcaggcttc tgttcgttat gacgttaacg agcaacgtct ggatatagac
     2101 gttcctcagg catgggtaat gaaaaattac caaaactatg ttgatccatc gttatgggaa
     2161 aatggcatta atgcggccat gttgtcatac aacctcaacg gatatcatag tgaaactcct
     2221 ggtcgaagaa atgacagcat ttatgctgct tttaacggtg ggatgaattt aggtgcatgg
     2281 cgactgcgtg cctcaggcaa ctacaactgg atgaccgatt ctggcagtaa ttatgatttt
     2341 aagaatcggt atatacagcg tgatatcgcc tcgctgcgtt ctcaactcat tcttggtgag
     2401 tcttatacaa cgggcgaaac ctttgattct gtcagtatcc gcggcattcg tttatacagc
     2461 gacagccgca tgttgcctcc gacattagcg agttttgcgc ctatcattca tggcgttgct
     2521 aataccaacg ccaaagtcac tattacgcaa ggtggctata agatttatga aacgactgtg
     2581 ccgccaggcg ctttcgtcat tgatgatctg agtccgtcag gttacggcag cgatcttatt
     2641 gttactgtcg aagaatccga tggctcaaag cggacattct cgcaaccttt ttcatcggtc
     2701 attcaaatgt tacgccctgg cgttggacgt tgggatatta gcggcggcca ggtcttaaaa
     2761 gatgatattc aggatgagcc taatttattt caggccagct actactacgg tctgaataac
     2821 tatctaacgg gttatactgg tattcagatt accgataata actataccgc tggtttgtta
     2881 ggtcttggtc tgaatacttc agtcggtgca ttttctttcg atgtgactca ctccaatgtt
     2941 cgtataccgg atgataaaac atatcaggga caaagttatc gtgtttcctg gaacaagtta
     3001 ttcgaagaaa caagtacttc actgaatatc gcggcctatc gctattcgac acagaattac
     3061 cttggtctta atgatgcact aactctaatt gatgaagtga aacatcccga acaagatctt
     3121 gaaccgaaat ccatgcgtaa ttactcgcgc atgaaaaatc aggttacggt cagtattaac
     3181 caaccgctaa aatttgagaa aaaagattac ggttcatttt atctttccgg aagttggtcc
     3241 gattactggg cttccggaca aaatcgtagc aattactcta ttggctacag taacagtgca
     3301 tcctggggca gctacagtgt cagtgcccag cgttcatgga atgaagacgg cgacactgac
     3361 gatagcgttt atctcagttt caccattcct attgaaaaat tacttggcac tgaacaacgg
     3421 acttcaggtt tccagagtat tgatactcaa atgagcagtg actttaaggg taataaccaa
     3481 ctcaacgtca gtagcagtgg ctatagtgat aacgctcgcg tcagttatag cgtaaatact
     3541 ggctatacga tgaataaagc cagcaaagat ttgagttatg ttgggggtta tgccagctat
     3601 gaatcaccgt ggggaaccct ggcaggttca gtttctgcaa atagcgataa cagtcgtcaa
     3661 gtgtctctca gcaccgacgg tggttttgta ttgcatagcg gcggcctgac cttcagtaat
     3721 gacagtttta gcgactccga tacactggcg gtagttcagg ctccaggtgc tcaaggagcg
     3781 cgaataaatt atggcaacag tactatcgat cgatggggtt atggtgtcac cagcgctctt
     3841 tctccttatc atgaaaaccg tatcgcgctg gatatcaacg atcttgagaa cgatgttgaa
     3901 ttaaaaagta ccagtgcagt agctgtaccg cgtcagggtt cagtcgtctt tgctgatttt
     3961 gaaaccgtgc aagggcaatc agccattatg aacatcacac gaagtgatgg taaaaatatt
     4021 ccatttgctg cagatattta tgatgagcaa ggcaatgtca ttggtaatgt tggacagggt
     4081 ggacaagcat ttgttcgtgg tattgagcag cagggaaata tcagcattaa atggctcgaa
     4141 gaaagtaacc ccgtaagttg tcttgcgcat tatcaacaaa gttcagaagc agaaaaaata
     4201 gcgcaatcta ttattctgaa tggaattcgg tgtcagattc agtaactaca agggacgtca
     4261 aatgataaaa ataacgccac ataaaatagc gatactgatg ggaatattat tatcaccatc
     4321 agtatttgca acggatatta atgtagagtt tacagccact gtcaaagcga caacctgtaa
     4381 catcacactt actggtaata acgtcacgaa tgatggcaat aataactaca cattgagaat
     4441 ccctaagatg ggtctggata agatcgcgaa taaaacgaca gaatctcagg ctgattttaa
     4501 actggttgct agtgggtgca gcagtggcat cagttggatt gataccactc tgaccggaaa
     4561 tgcatcatca agctcaccta agcttattat accgcagtct ggtgattcat cttcgacgac
     4621 aagtaatatc ggtatgggtt tcaaaaaacg gactactgat gatgccactt tccttaaacc
     4681 taacagtgcg gaaaagatac gctggagcac agacgagatg cagcccgata agggtcttga
     4741 aatgaccgtt gcgctacgtg aaacagatgc agggcaaggc gtaccgggga attttcgtgc
     4801 actggcaacg tttaatttca tctatcaata agaaggataa ttaagatgac atttaaaaat
     4861 ttacgttatg gattatccag cagtgtcgtt ttggctgcct cattgttcag cgtaccctct
     4921 tatgcggcaa cagacagcat tgggctgacc gtgattacta ccgtagaaat aggtacttgt
     4981 accgctacat tagtaaatga ctctgatcag gacatttctg ttgttgactt tggcgatgta
     5041 tatatttctg aaatcaatgc caagaccaaa gttaaaacat tcaaactcaa attcaaagac
     5101 tgtgcgggta tccccaataa aaaagcacaa ataaaattaa ccaagcgagc cacatgcgaa
     5161 ggtactgcta atgacggtgc ggggttcgca aatggatcca cagccgcaga taaagcaagt
     5221 gccgtcgccg ttgaagtctg gagcaccgaa actccggcaa cagggagcgc aacacaattt
     5281 agttgtgtga caccagcatc tcaagaggtc actatctcca atgcagccaa cgcggttgtt
     5341 tattatccga tgagtgctcg actggtcgtc gaaaaaaata aaaccgtaag caatgtcact
     5401 gcgggtaaat tttctgcacc tgccacattt acagtaacct ataactaaca gactgacaga
     5461 tccaggaact atatatgcat cccactcaac gtaagctgat gaagagaata attctgtttc
     5521 tgtcactaca gtttcccatt acctatccag ccatcgctgg gcaggatatt gaccttgttg
     5581 ccaatgtaaa aaacagcact tgcaaaagcg gaatcagtaa ccagggtaat attgatcttg
     5641 gcgtcgttgg ggtgggatat ttttcagata atgttactcc tgaaagctat caaccaggtg
     5701 gaaaagagtt cactgtcaca gtatcagact gtgcattaca gggaactggc gatgtgctta
     5761 atcagttaca tattgatttt agagccctta gcggtgtcat ggctgcaggt tcaaggcaaa
     5821 tatttgccaa tgaagtcgca accggggcta aaaatgtcgg tgtcgttctc ttttccattc
     5881 aggacccgac caatatattc aatgttatta gttcagcagg aaattctcgt tccgtgtatc
     5941 cagtaatgac ttctgccctg cataattctt catggaaatt ttatgccaga atgcaaaaaa
     6001 tcgatcccgc gctggatgtc atatctggcc aggtaatgag tcatatatta gttgatgtgt
     6061 actacgaata atagatcaaa acaaataact gcgactgtta cagcagtaga tggtggagtg
     6121 ataatgaaga aattcttcag acattttctt tttttaattc tgtgcttgag ttgttatacc
     6181 gcgagtgctg gcactgatga taacgtagga tatattgttg ggaatagtta cggcgttggt
     6241 ccgtcagatc aaaaatggag agaaacgggg ccgaatggcg atgctactgt catattcaga
     6301 tatgcaacaa gtacaaataa tttagttttc tacaaaccga cgcagcttgg acctacaggt
     6361 gtaaaattac agtggagtca gttagatacc gcttctggtg gtggttttct ttattgcaac
     6421 agaagtgata gcacaagtgg tagcgcaatg cgtattgaaa atgcaatggt tgactcaggt
     6481 aaaatgtatg gctcccataa attatttaat acatcagttc ctggtttgta ttacacatta
     6541 ttaatttcaa acatgtggtc agcttacggt accgtaacta acgttagttc acctgggata
     6601 tacattggtg actctgcaga acaatatttt tcgtggtata atccaagcga agacgtgtta
     6661 tattggagtt gcaataatgc gaatagcacc cgtaaatact gggctgtagg tggtatttat
     6721 cagaccctta caattgaatt ctatacagat acaaactttg atccaacagt aactcaacag
     6781 atcaaattat ccagctcatc aaattatctg tattcattta aagcctatgg cgcaggctaa
     6841 ggtataaatg agcatagtta ttttatcaaa atcgatttcg atctgctcaa tgtcaagttg
     6901 actaatccca cttgttttac cgctatgctt agtggaactt ctgtgactgg ttctacggta
     6961 aaaatgggtg aatatagtgc agaacaaatc agaaacggtg ccacaccggt tccttttgat
     7021 atttcacttc aaaactgcgt tcgtgtgact aatattgaga caaaattagt ttcaacaaag
     7081 gttggtactg aaaacgggca actccttggt aatactctca caggtaatga cgcagcaaaa
     7141 ggagtcggtg tactcataga aggtttagca actagtaaaa atcctctaat gacattgaaa
     7201 cctaatgatt caaattctgt ttataaagat tacgacccga gaggcaaaga cgatacaaca
     7261 ggaggagttt acccggatca agatacaggt ataacatacc ctctccattt ccaggccacg
     7321 ctacaacagg atggaactat accaatagaa gctggtgagt ttaaagccac cagtactttc
     7381 caggtaacct acccttaata agtccaccgc accgccatca tctggcggtg cagtaattga
     7441 taaatcattg cccccaccaa tgtgactgat aaaatgccag gcaacacgac atcatttatc
     7501 aggatacgtt atgaaaccaa ccaccattgc ttcacttcag aaatgtaagc aggataaaaa
     7561 gcgtttcgcg accatcaccg cttacgacta cagcttcgcg aaactatttg ctgatgaagg
     7621 acttaacgtc atgctggtag gcgactcgct gggcatgacg gttcagggtc acgactccac
     7681 cctgccggtg accgttgctg atatcgccta ccacactgcc gccgtacgtc gcggcgcgcc
     7741 aaactgcctg ctgctggctg atctgccgtt tatggcgtat gccacgccgg aacaagcctt
     7801 tgaaaacgcc gcaacggtta tgcgtgccgg tgccaatatg gtcaaaattg agggcggtga
     7861 gtggctggtc gaaaccgtaa aaatgctgac cgaacgtgcc gttcctgtat gtggtcactt
     7921 aggtttaacc ccacagtcag tgaatatttt cggtggctac aaagttcagg ggcgcggcga
     7981 tgaagcgggc gatcaactgc tcagcgatgc attagcctta gaagccgctg gggcacagct
     8041 gctggtgctg gaatgcgtgc cggttgaact ggcaaaacgt attaccgatg cactggcgat
     8101 cccggttatt ggcattggcg caggcaacgt cactgacggg cagatcctcg tgatgcacga
     8161 cgccttcggc attaccggcg gtcacattcc taaattcgct aaaaatttcc tcgccgaaac
     8221 gggcgacatc cgcgcggctg tgcggcagta tatggctgaa gtggagtccg gcgtttatcc
     8281 gggcgaagaa cacagtttcc attaaggagt cacgttgtgt taattatcga aaccctgccg
     8341 ctgctgcgtc agcaaattcg ccgcctgcgt atggaaggca agcgcgtggc gctggtgcct
     8401 actatgggta atctgcacga tggccatatg aagctggtcg acgaagccaa agcccgcgcc
     8461 gatgtggtcg tcgtcagtat tttcgttaac ccaatgcagt ttgaccgccc ggaagatctg
     8521 gctcgttatc cacggacctt gcaagaggac tgcgaaaaac taaacaaacg taaagtggat
     8581 ttagttttcg ccccttcggt aaaagagatc tacccgaacg gtactgaaac ccacacttac
     8641 gttgacgttc ctagcctttc gaccatgctg gaaggtgcca gccgtccggg acattttcgc
     8701 ggcgtttcga ctattgtcag caagctgttc aacctggtcc agccggacat cgcctgcttc
     8761 ggtgaaaagg actttcagca actggcgcta atccgcaaaa tggttgccga tatgggcttc
     8821 gatattgaaa ttgtcggcgt cccgattatg cgcgccaaag atggccttgc gctcagttcc
     8881 cgtaatggtt atctgacggc ggaacaacgc aaaattgcgc ccggtctgta caaagtttta
     8941 agttcaattg ccgacaaatt gcaggctggg gaacgggatc tcgatgaaat tattaccatt
     9001 gcggggcaag aactgaatga aaaaggcttc cgcgccgatg atattcagat tcgcgatgcc
     9061 gacacattgc tggaagtttc tgaaaccagc aaacgggcag taattctggt agccgcctgg
     9121 cttggcgatg ctcgcctgat cgacaacaaa atggtcgagc tggcgtaata cttaactggc
     9181 gctacggctg atggcgccag ttattaattt accccacgtc atctgtcata acacaggtaa
     9241 cttaaataag gttaattacc ttatcaatct cagcaagagg taaatttgcc atctctgcaa
     9301 cgtcttcccg agacattcct ttacttagaa gacgcagggc gaattcttgc ctttcctcct
     9361 gtcttccctg ctgaatcccc ttctcaatcc ctttctcttc aaaccactgc gccagcgtca
     9421 tcatagactc ccctcccgtt tccctgtctc tcagcacacc ataaaacaaa tccgcttgtt
     9481 cagtatgacc gcgttgcagc atatagtttt gcatggcaac taactgactt ccgctagtgt
     9541 acccttcgtc aatcagcgtg accagttgct ccagcaataa cattaagtcg cgctggcgaa
     9601 tatgtttttg cagtagttcg agaatcgcaa tccgccgatg ttgcatgatt tcgtcatccg
     9661 gtgtgatggt gatatccacc agcgggaaag gactgttata gacgcgtcgt gccagctccg
     9721 gcgagtaaaa tatatcaaac cagcacattg agagcggata aggtgtggcc tcgccctgat
     9781 aaaacagtat cggcaccacc agcggcagct tatcgtgatc cgcctccaga tgccggtgca
     9841 tggcagcaat agaataacgc atcatgcgaa aggccatttt cttatccggc ttgctttggt
     9901 gttcaatcac aacatgcaga taaccgggat tgccctgcat ttgcacggaa tagagcacgt
     9961 ccgtgctgtg tcctttcagg ctctcttcaa tgaaactccc cgactctaaa tgaagcgtgt
    10021 tgaggtcaca aagttctcgt aattccactg gcaaatgtat ctccagaaag tcgcgagccg
    10081 tctccgcgtg cattaaaaat tgtttaaaca ctgcatcatg cggtgtggta ctcggtgcat
    10141 ccatcttccc tgccttccgt gttgtcttgc gtgaaggcag tatccagtca aggcgtgcca
    10201 tcgtcagcca cctctttagt gtttaccgag cagcgttcag agaaatttca attggttaca
    10261 gccgttacat ggcacgcttc ggcacggtac gcaaactgct gatgtaaatg ccgtcagatg
    10321 agtagacact aaacaaaaat cgggcaatac tgcgtgagaa tttcctcaaa gcaggcatcg
    10381 cctgcttcgt taacgacagg gtagaaaggt agaagttatg attcgcacga tgctgcaggg
    10441 caaactccac cgcgtgaaag tgactcatgc ggacctgcac tatgaaggtt cttgcgccat
    10501 tgaccaggat tttcttgacg cagccggtat tctcgaaaac gaagccattg atatctggaa
    10561 tgtcaccaac ggcaagcgtt tctccactta tgccatcgcg gcagaacgcg gttcgagaat
    10621 tatttctgtt aacggtgcgg cggcccactg cgccagtgtc ggcgatattg tcatcatcgc
    10681 cagcttcgtt accatgccag atgaagaagc tcgcacctgg cgacccaacg tcgcctattt
    10741 tgaaggcgac aatgaaatga aacgtaccgc gaaagcgatt ccggtacagg ttgcttgatt
    10801 gttatccctg cggctggtta ctcaccagcc gcgacatcgt ctccagcgaa tccgttctta
    10861 agatataaag tcgttttagt aacaacggat tatcccccgg ttttactttg cctttcatgg
    10921 ttgtcaccgc caggtgaaat ccggcatcgt ttgctgcctt cacggctttg tcattaaatc
    10981 cgccaaacgg atacgaaaga taccagacat gcggattaaa ttgcgccaga gcgcggcgtg
    11041 aacgtgcaaa atcaaacaga atattgtgct cactacggct cagtaatatg ggtcggcgat
    11101 agccatccac ccgatgcaaa aaatgggtat gtgactgaaa atcaaaaaca tcgcgaattt
    11161 cgttaagctc agaaacgctc ataaattgca gcgattttgg gttccacttc tgcgggtgac
    11221 gtttgatgcg cgaggtaaca acaaacgccg tcgccttcat gccatattgt ttcaatacag
    11281 gatacgcata gcggctcacc gacttgaggc catcatcaaa ggtaatcacc accgctcgcg
    11341 cagggagatt gatcttattc ttcacgtaac cttccagctg cgccatgctc agtgtcgcgt
    11401 atcccctgtc gcgcagccag gccatttggt tattgaaagc gcgtaccgag gtggtcgtcg
    11461 aagtatggcg aaaacgggtg ttttcttcat cgcgcagaat atggtgatag gttagcaccg
    11521 gcaggccatt atcgggttgg gcatccagcg cgctgatata ggcgagtcga tcgccaatac
    11581 ggatctgata ccaggtttga tttaacctgt ctttcagttt atgcaaaatc gggtagcgca
    11641 aattgtccgc cagtacccca aatggcgcac tgcccgcact cggcgcgtta tagaccggcg
    11701 tatctttcca ggtaattaag ttctgattac tcagcggctt gttgaggtca cccaaaccgt
    11761 cttcaacttt ttgtcgcccc tgaactggct cgagatgccc tttatcgata aaccctttac
    11821 caaagccaaa attaaatgcg taataacttg cggcagtggg ctccacggca atgatttgcc
    11881 cggcccgaat attccccacg gtcaccatct tgtcgccaat ttgcgcccag atcgcagcat
    11941 tttcgatggt ttgcatataa cgggcaggta acgcggcact gacacttgcg gtaaacagca
    12001 tcagcaggag aataacagct tgtttgtaca tgaaaaaata accgagggtg aaacaacgct
    12061 tttattgtag caaaagcgct ataagaatcg tgggttaata ctcatacaaa ttatgtttga
    12121 ggacgaaagg cggatttttt tgttgttggt gccaaagact actcacctcc ggcccaccca
    12181 gttcgacaat acgctcgcga aactctgaac tggtcatggt ttcacggcaa gccatcatct
    12241 gttcaattaa cggtaacgtg acaccagaaa tcacttcaca acgggagtgt ttatggctta
    12301 ataatgaagc cacgcggtac ggtggcgctc cggctaaatc ggttaagaag atgacaccct
    12361 caccagagtc cgcttcatgc agagcatcgc acatcatgcg gctgagcata ttagagctta
    12421 atccgcgcca gaaattcacg gcccggcact gaagaagtgc cccatgtttt ttctccagcg
    12481 catccagtat ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct aacatcgcct
    12541 tcctccttag ccgaaaatca gtctaccctg accattgttg ataaagggtg atagccgtca
    12601 aaactcctct cccccgaatc cagaggagag gaaatagcct tagctacgca aaccacgtcc
    12661 acgttggatc agcgaccaac agatcaaata aaacgccaca ataaagacca ccagtacgcc
    12721 aaaggtagtg accagcggca catcatttat accgaggaag ccgtagcgga aaccactgat
    12781 catataaacg attgggttca ggtgcgacag cccttgccag aacggcggca acaaagtcag
    12841 ggagtaaaac accccgccca aatacgtgag tggcgttaac acaaaggttg gcaccaggct
    12901 gatgtcatcg aacgttttgg caaacacgcc gttcagcaaa cccgcaaggg agaatagcac
    12961 cgccgtgagc accagggtta aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa
    13021 aaacagtgaa attgccgtca ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc
    13081 gacatatccg gcaataatga cgtgagtcgg aaccggcgct accagcagct cttcaatatt
    13141 acgctggaac ttggcaccaa aaaatgatga cgcgacgtta gcgtaggcat tggtgatcac
    13201 cgacatcatg atcagccccg gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc
    13261 aatacgcgaa cctatcaggt taccgaagat aataaagtaa agggtcatgg tgatgactgg
    13321 cggcaccagc gtctgcaccc agatacgcat aaagcgatgg atctctttcg cccagatgct
    13381 ttttagcgcc acccagtaaa gatgcatcat gcgcgatctc cttgtttttc attaaccagt
    13441 gaaacaaaca gctcttccag acggttagct ttgttacgca tacttaatac ctgaatgccc
    13501 tgctcactta actgcgtaaa tacgctgttg atcccctgct cacgcagcac ttcaacttcc
    13561 agcgtcgcgg tatcgaccag tcgatactga tagccatcga gcttcggtaa cggacttttc
    13621 ggtgcgagat cgagaataaa ggtttccgat ttcagcttag ccagcagcgc cttcatcgag
    13681 gtattttcca ccagctcacc gtgttgaata atgccgatat tgcggcacag catttctgct
    13741 tcttccaggt agtgtgtggt gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa
    13801 aagccccaca ttgagcggcg aagttcaata tccacgcctg cggtcggttc gtcgagaatc
    13861 agtagtttag gttcatgcat taacgcacgg gcaatcatta aacggcgctt catcccgcca
    13921 gataacatac gcgcacgttc gttgcgtttt ccccatagat cgagctgctt aagatatttt
    13981 tcgctgcgga tgtacgcctc tttgcgctcc acaccgtagt accctgcctg attcaccaca
    14041 atttgctgca cggtttcaaa cgggttgaag ttaaattcct gcggcaccag tcccaactga
    14101 cgtttagcgt tcacgacatc cttctcgaga tcgtaaccaa atacgctgac ccgcccggag
    14161 gttttattta ccagagagct gataataccg atagtggtcg atttcccggc cccgttcggc
    14221 ccgagaagcg cataaaaatc acccgcttcg acctgcaaat ctatcccacg aagcgcctga
    14281 acgccgcctg gataggtttt tttaagctgt tgaagttcca gtgcaatggt cataaatttt
    14341 tacttacctt acgttcttac actttatctg tggtttaaat cgtcccggag ttgccctata
    14401 ttagccaaac gtaattattt ggttacaggt cgttaacctc catgaaagac atagatacac
    14461 tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga agaggatccc gggttttttg
    14521 agaaactggc acaagcgcaa aaaccgcgct ttctatggat tggatgttcc gacagtcgcg
    14581 ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg
    14641 ctaacctggt cattcacact gacctgaact gcctttccgt ggttcagtat gcagtggatg
    14701 tactcgaagt tgaacacatt attatctgtg gccactacgg ttgcggcggc gtacaagccg
    14761 cagttgaaaa cccggaactg gggcttatca acaactggct gctgcatatc cgcgatatat
    14821 ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt
    14881 gtgaactgaa cgtcatggaa caggtgtata acctgggcca ctccaccatt atgcaatcag
    14941 cgtggaaacg cgggcagaaa gttaccattc acggctgggc ctacggcatt cacgacggct
    15001 tgctgcgtga tctggatgtt accgccacca accgcgaaac ccttgagcaa cgttaccgtc
    15061 acgggatttc caacctcaag ctgaaacacg ccaaccacaa ataaaaatgc catgccggat
    15121 gcaacacatc cggcaacttc acacttactc gtccagcaga atcactttgc cgatatacgg
    15181 cagatgacgg taacgctgtg cgtaatcaat gccgtaaccc accacaaact catccgggat
    15241 cgagaaaccg ataaattcta ccgggacgtt cacttcacga cgggacggtt tatccagcag
    15301 cgtacaaatc gccagcgact tcggttcgcg caggcttaag atctcacgca ctttcgacag
    15361 tgtattcccc gagtcgatga tatcttcaac aatcagcacg tccttgccac ggatatcttc
    15421 atccagatct ttgaggattt tcacatcacg ggtggtggac atgccgctac cgtagctgga
    15481 ggcggtcata aagtcgactt catgagatac ctgaacttca cggcacaggt ccgccataaa
    15541 cataaatgag ccacgcagca gacccaccag caccatatcg ctgccgctgt ctttgtaacg
    15601 ctcagtaatc tgacgaccca gttcggcgat acgcgcttta atctccgctt cggggatcat
    15661 tacttctaca gtatgtttca tatctctaac catatgattt aaaaacaaat cacttcgcac
    15721 gggcaggttt tggcaccgca acaaagcgca agacacgcag tataccagta aaactattca
    15781 ttcgcggaat ctgttaataa agctcgaatt gtgatgacga tcacacatgt taaaacctat
    15841 aattaattgc tactaaaata ttaatgaatt gaaatggtgt ctctttatgg caattaacaa
    15901 tacaggctcg cgacgattac tcgtcacgct aacagccctt tttgcagcgc tttgcgggct
    15961 gtatctactc attggcggag gctggctggt cgcgattggc ggctcctggt actaccctat
    16021 cgctggcctt gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct
    16081 ttggctatac gccgccctgc tgctcggcac catgatttgg ggcgtctggg aagttggttt
    16141 cgacttctgg gcgctgactc cgcgcagcga tattctggtc ttcttcggca tctggctgat
    16201 cctgccgttt gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg ccgcactggt
    16261 ggtggcactg ctgattagcg gtagtatcct gacctgggcc ggatttaacg atccgcagga
    16321 aatcaacggc accttaagcg ccgatgccac acctgcggaa gctatctcac ccgttgccga
    16381 tcaggactgg cctgcctatg gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca
    16441 aattaacgcc gataacgtcc ataatctgaa agaagcctgg gtgttccgta ctggcgatgt
    16501 gaagcagccg aacgatccgg gcgaaatcac caatgaagtg acgccgatta aagtgggcga
    16561 caccctttac ctgtgtaccg ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa
    16621 agagaaatgg cattacgatc ctgagctgaa aactaacgag tctttccagc acgtaacctg
    16681 ccgtggcgtc tcttatcatg aagccaaagc agaaacggct tcgccggaag tgatggcgga
    16741 ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcga ctgattgcga ttaacgctga
    16801 aaacggcaaa ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat
    16861 gccagacacc aaaccgggtc tgtatgaacc gacttcgcca ccgattatca ccgataaaac
    16921 catcgtgatg gcaggttcag ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat
    16981 ccgtggtttt gatgtcaaca ccggggagct gctgtgggct tttgatcccg gcgcgaaaga
    17041 tccgaacgca atcccgtctg acgaacacac ctttaccttt aactcgccaa actcgtgggc
    17101 gccagcggcc tatgacgcga agctggatct ggtctatctg ccgatgggcg tgaccacgcc
    17161 ggatatctgg ggcggtaacc gcacaccgga acaggaacgt tatgccagct cgattctggc
    17221 gctgaatgcc actaccggga aactggcgtg gagctaccag accgttcacc acgacctgtg
    17281 ggacatggat cttccggcac agccgacgct ggcggacatc accgttaatg gtcagaaagt
    17341 gccagttatt tacgctccgg cgaaaaccgg caacattttt gtgctcgatc gtcgcaatgg
    17401 cgaactggtg gttccggccc cggaaaaacc ggtgccgcaa ggtgctgcga aaggcgatta
    17461 cgttactccg acccagccgt tctctgaact gagcttccgt ccgaagaaag atttgagcgg
    17521 tgcggatatg tggggagcca ccatgtttga ccaactggtg tgccgcgtga tgttccacca
    17581 gatgcgctat gaaggcattt tcacgccgcc atctgaacag ggcacgctgg tcttcccggg
    17641 taacctggga atgtttgaat ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat
    17701 tgccaaccca atggcactgc cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc
    17761 gatggagcag ccgaaagatg ccaaaggcac gggtacggaa tccggcattc agccacagta
    17821 cggtgtaccg tatggtgtca cgctcaaccc gttcctctca ccgtttggtc tgccgtgtaa
    17881 acagccagca tggggttata tctcggcgct ggatctgaaa accaatgaag tggtgtggaa
    17941 gaaacgtatt ggtacgccgc aagacagtat gccgttcccg atgcctgtac cggtgccgtt
    18001 caatatgggt atgccgatgc tgggcgggcc aatctccacg gcgggtaacg tgctgtttat
    18061 cgctgctacg gcagataact acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg
    18121 gcagggccgt ttaccagcgg gtggtcaggc gacgccaatg acctatgaag tgaatggcaa
    18181 gcagtatgtg gtgatctccg caggcggtca cggttcattt ggtacgaaga tgggcgacta
    18241 tattgtggct tatgcgctgc cggatgatgt gaaataacca taatgcccgg agagatccgg
    18301 gcatatttcc gaatacggtc tttttatacc gtaaacccta acatcatccc cgtatcttca
    18361 tgctccagca gatggcagtg cgccatataa gcatgttctt tcggtgcgtt gtgattaaac
    18421 ttcaccagca cttcactgac attgccttct accttaacgg tatctttcca gcccgcgcga
    18481 tgagccgctg gcggtttgcc attttctgac aagatacgga actgggtgcc gtgaatatgg
    18541 aacggatgca gcatcatgtc gcccacgcca gagataaccc aacgttcgta ctgccctttc
    18601 gccgccgcaa acatcggctt attcatatca aacgcctgac cgttgatttt attggcatgg
    18661 tggaaatcga acttcccgcc atggttcata tgattcatat tgccgtgccc catatggccc
    18721 atcatctggc tatgatccat cccggccatc gcctgatcgc catatttctc catcagcatc
    18781 tgcatcccca tcatatcgag catcgggtcc atagaaagtt gcagcttgcg taccgtcagc
    18841 ccttccagag aaggtaacgc aggcaggcta cttaatgtgt ctggcaaagc accggaggca
    18901 ctaatagcaa tcggctgaat ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc
    18961 atccccatct ggctgaccgg tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc
    19021 accagcactt caaaacgctc gcccatcagc accggcagct cgctcacctt caccggttca
    19081 ggtagcagac caccgtcgct ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa
    19141 ttgagcgagc gggcattaca gccattaagc aaacgcaggc gcagccaacc acgcggggca
    19201 gcgtgttgtg ggtagattgc accgttggtc agcagcgtat cgccaaacca gcccacggcg
    19261 gcggtcatca catccagttg ataatcaatc tgcccatcag cgttaaattt tttatcctga
    19321 acaatcaccg gaacatcatc gatgccccac tgttttggta gcattaattt caggatctcg
    19381 tcatcttcaa ttaccaccag cccagccaac cccatcgcca cctgtcgccc ggtcttgcca
    19441 tgttgatgtg gatggaacca gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag
    19501 cgcttgccac ctggcagaat aattccctgc gggccgccgt cgacttcacc cggtacttcc
    19561 agcccgtgcc agtgcaacgt cgtctcttcc gtcagttggt tatagatatc aaccgttacc
    19621 gctttgccgc gctgtaattt caccgccggc cccagcagat tgccgttata gccccaggta
    19681 gttgcagttt tctcgccaaa ggtagactga cctgcgccaa tagttaactg aatgcgatta
    19741 cgggcatcgg tcgtgagcaa atcagggatc ggtaacgttg ggcgttccgc cgcaaagact
    19801 gcgcggctcc acagcggcaa ggctgaagcc acacccagcg cgacggaata ttttaagaaa
    19861 tcacgacgtt gcatagttat ttccttattc ttaagcaggc gaaacaaaat caaacgttga
    19921 gcatagtcct tccccttacg ggaaggtcaa gccgcagaca caatttaatc gacaataatg
    19981 gtcgtcgcct cgctcgcagt gtgctaacgt ttatcttctt taagcccctg gtagaagcaa
    20041 tgaagacgtt tttcagaaca gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt
    20101 acgcgctcag cgagtctgaa gccgaagata tggccgattt aacggcagtt tttgtctttc
    20161 tgaagaacga ttgtggttac cagaacttac ctaacgggca aattcgtcgc gcactggtct
    20221 ttttcgccca gcaaaaccag tgggatctca gtaattacga caccttcgac atgaaagccc
    20281 tcggtgaaga cagctaccgc gatctcagcg gcattggcat tcccgtcgct aaaaagtgca
    20341 aagccctggc ccgcgattcc ttaagcctgc ttgcctacgt caaataatcc ctgatacctt
    20401 tttgtagaaa tattgaccgt gcatccgcgg tcaatgttag ctattatgtt gcgccctttt
    20461 tttacgggtg ttaacaaagg aggtatcaac ccatggccga aaaaaaacag tggcatgaaa
    20521 cgctacacga ccagtttggg cagtactttg cggtagataa cgttctgtat catgaaaaga
    20581 ccgatcacca ggatctgatc atttttgaga acgctgcatt tggtcgcgta atggcgctgg
    20641 atggcgtagt acaaaccacc gagcgcgacg agtttatcta tcatgagatg atgacccatg
    20701 ttccgctact ggcccacggt cacgcgaaac atgtgctgat tatcggcggc ggcgacggtg
    20761 ccatgctgcg tgaagtaacc cgacataaaa acgttgagtc aatcacgatg gtggaaatcg
    20821 atgcgggtgt cgtgtcgttc tgccgtcagt atctgcccaa ccataacgcc ggtagctacg
    20881 acgatccgcg ctttaagctg gtgatcgacg atggcgtcaa tttcgttaat caaaccagcc
    20941 agacctttga tgtcattatc tccgactgca ccgatcctat cggtcccggc gaaagtctgt
    21001 tcacctcagc attttatgaa ggctgcaaac gttgcctgaa tcctggcggt atcttcgtcg
    21061 cacaaaacgg cgtctgcttt ttacagcagg aagaagccat cgacagccat cgcaaactca
    21121 gccattactt cagcgacgtt ggcttttatc aggcggcgat cccgacctat tacggcggta
    21181 tcatgacttt tgcatgggcg acagataacg acgccttacg ccatctctca accgaaatta
    21241 ttcaggcgcg ttttctcgct tctggcctga aatgccgtta ttacaatccg gcaatccata
    21301 cggcagcttt tgccttacct cagtatctgc aagacgcact ggcttcacag ccgtcctaag
    21361 gagaagataa gaaattgaaa aaactgaaac tgcatggctt taataatctg accaaaagtc
    21421 tgagtttttg tatttacgat atctgctacg ccaaaactgc cgaagagcgc gacggttata
    21481 ttgcttatat cgatgaactc tataatgcca accgtctgac cgaaatcctg tcagaaacct
    21541 gttccattat cggggccaat attcttaaca tcgcccgcca ggattacgaa ccacagggtg
    21601 ccagcgtcac tattctggtg agtgaagaac cggttgaccc gaaactcatc gacaaaacag
    21661 aacaccccgg cccactgcca gaaacggtcg ttgcccatct cgataaaagt catatttgcg
    21721 tacataccta cccggaaagt catcctgaag gcggtttatg taccttccgc gccgatattg
    21781 aagtctctac ctgcggcgtg atttctccgc tgaaggcgct gaattacctg atccaccagc
    21841 ttgagtccga tatcgtaacc attgattatc gcgtgcgcgg ttttacccgc gacattaacg
    21901 gtatgaagca ctttatcgac catgagatta attcgattca gaactttatg tctgacgata
    21961 tgaaggcgct gtatgacatg gtggatgtga acgtctatca ggaaaatatc ttccatacca
    22021 agatgttgct taaagagttc gaccttaagc actacatgtt ccacaccaaa ccggaagatt
    22081 taaccgacag cgagcgccag gaaattaccg ctgcgctgtg gaaagaaatg cgcgaaattt
    22141 attacgggcg caatatgcca gctgtttaac ggctctggcg gagctcccag gctccgccag
    22201 atttatttac ttctgctgca cgaaattgcg gtaagccgcc acgacctgca gaaaatcctc
    22261 aacgccgcat agcgacaggc tttcttcgtc gtagtagttc atcccctctt ccatttcatc
    22321 gccagcgaat tccagttgat tggcgcgaac catcacctct tcaccgtcca tccacagggt
    22381 gtattcatgc cctgcccgtt gccaggaccg ttcgctacct ttcagtgcgt gcgctgcttg
    22441 ttccacttca tcaagcaagg ccaggttttc tttcacctct tcattaaacc agtgcccgac
    22501 cacttcatgc cccatggaca tacgcacctt taccactccg gtaatatcgc gcagaaattc
    22561 gtaatccata gtgtgttcct ctgctcccgg caatgcgccg aaatcgcatt gcgccattga
    22621 tgtaattatc gcagccttac cggagaaaaa cagcggaacg gcgatggttt agaaataaaa
    22681 aatgcccggg aatctcattc accgggcatt gtgtcgttta tgcgcagcgc gtgcgcttac
    22741 ttattacacc gcagtctgga aaatcacccc atcggctttt tcggtgtact gagaaagctg
    22801 gttgaagttc agataacgat aagtatcaac ggcagtttta tctacttgcg caacgtaggt
    22861 ctggtactct tccggcgtcg gcagtttgcc aatcagcgcc gcaacagccg ccagttctgc
    22921 agaagccagg aagacattcg cgccagtccc cagacggttc gggaagttac gggtagaggt
    22981 ggaaaccacc gtcgcaccgt ctgctacacg cgcctggtta cccatacaca gggaacagcc
    23041 agggatctcg atacgcgcac cgctcttacc gaagacgcta tagtagcctt cttcggtcaa
    23101 ctgcgcggca tccatacggg ttggcggtgc cacccacagg cgcgtcggca actggccttt
    23161 gtgcgcatcc agcagtttac ccgcagcacg gaagtgaccg atgttggtca tgcaggaacc
    23221 gataaacact tcgtcgatct tctcgccctg taccgcagac agcggacgtg cgtcgtccgg
    23281 gtcgttcggt gcacagagga ttggctcttt aatatccgcc agatcgatgt cgatcactgc
    23341 cgcgtattcc gcatctgcat cggcttccag cagctcagga ttcgccagcc atttttccat
    23401 gccctgaata cgacgttcca gggtacgacg atcgccgtaa ccttccgcga tcatccactt
    23461 cagcaggacg atgttagagt tcaggtattc gatgatcggt tctttgttca gcttgatggt
    23521 acaaccagcg gcagaacgct cggcggacgc atcggtcagt tcaaatgcct gctcaacttt
    23581 cagatccggc agaccttcaa tttccagaat gcgaccagag aagatgtttt tcttgccttt
    23641 cttctcaacg gtcagcagac cttgtttgat cgcatacagc gggatcgcgt gtaccagatc
    23701 gcgcagggtg atgcccggct gcattttgcc tttgaagcgc accagaacgg attccggcat
    23761 atccagcggc attacgcctg ttgcggctgc aaatgccacc agaccagaac ccgccgggaa
    23821 agagataccg atcgggaaac gggtatggga gtcaccaccg gtaccgacgg tatccggcag
    23881 cagcatacgg ttcagccagg agtggattac gccgtcaccc ggacgcagcg acacaccacc
    23941 acggttcata atgaagtccg gcagcgtgtg gtgcgtgttc acgtcaactg gcttcggata
    24001 cgccgcggta tggcagaaag actgcatcac caggtcagcc gagaagccca ggcacgccag
    24061 gtctttcagt tcatcacggg tcatcgggcc ggtggtgtcc tgtgaaccta cagaagtcat
    24121 tttcggttcg cagtacgcgc ccggacgaat acctttcaca ccgcaggcac gacctaccat
    24181 tttctgcgcc agcgagaagc cgcgatcgct ctcagcgaca tctttcgcct gacggaacac
    24241 atcactgtgc ggcagaccaa gtgcttcacg cgctttggtg gtcaggccac gcccgataat
    24301 cagcgggata cggccgccag cacgcacttc atcaatcagc acgtcggttt tcagttcgaa
    24361 ggtcgccagc agttcgccgg tttcgtggtt acgcacttca cctttgtacg ggtaaacgtc
    24421 aatcacgtcg cccatgttca ggttagagac gtcgacttcg attggcagtg cacccgcgtc
    24481 ttccatcgtg ttaaagaaga tgggtgcaat tttaccgccg aggcacaaac caccgccgcg
    24541 tttgttcggc acatgtggaa tatcatcgcc cataaaccac agaacggagt tcgtggcgga
    24601 tttacgcgat gaacccgtac ccacaacgtc accgacgtac gccagcggga aacctttctg
    24661 ttgcagagct tcaatctgtt tgatcgggcc aacaacacct ggctggtctg gctcaatacc
    24721 ttcacgggcg tttttcagca tcgccagcgc gtgcagtggg atatccgggc gcgaccacgc
    24781 atccggtgcc ggagaaaggt catcggtgtt agtttcgcca gtgactttga agacagtaac
    24841 ggtcagtttt tcagccagcg ccgggcgatt caggaaccat tcggcatccg cccaggactg
    24901 cataacctgc ttcgcatatt cgttgcctgc tttcgctttc tcttctacgt catagaagtt
    24961 atcgaacatc agcagcgtgt gagaaagtgc tttggcagca ataggtgcca gtttggcatc
    25021 atccagcgcg tcgatcagcg gatgaatgtt gtaaccaccc tgcatggtgc ccagcagttc
    25081 gatggctttt tccggagtca gcagagggga tttggcttcg cctttcgcga tagcagccag
    25141 gaagcctgct ttgacatagg cggcttcatc gacgcctggg ggaacacggt tggttaacag
    25201 atctaacagg aattcttctt cgcccgcggg cgggtttttc agcagctcta caagtgcggc
    25261 catttggttt gcatccaggg gtttgggcgc aatcccctca gcggcacgct cagctacgtg
    25321 cttacggtat tcttctagca cgacggttct cctcgctctc attgtcatag tgcggcaggc
    25381 gattctcttc acgctcctgt gagacagcag tttgtagggt aaatgcccag taccgcaacg
    25441 ggcagaatag caggattttg acgatgtgtt aatctgttta caaaaaagca acataaaaaa
    25501 gtggctgaat cgttaaggat ggtctagaga ttgtttccct ccgtaaaaac ttcgttaatt
    25561 cgcatggtga taatcacctt tctcaacgaa tcccaagatt agtcaaaatt taaactaccg
    25621 cctctttata ctcggattca cagcacctgc gggtggcagt tcgccgacca ttgcgatttc
    25681 cttgagatcc gaattatgaa aatgactttg ccgtttaaac cccatgtgct ggcgctaatt
    25741 tgcagtgccg ggctttgtgc cgcctctacc gggctatata taaaaagccg cacagtggaa
    25801 gcgcctgtgg aaccgcaatc gacacaacag actgcgcctg acatcaccgc agttacgctt
    25861 cctgcaacgg tttccgcacc gcccgtaacg cccgccgtcg tcaaatccgc attcagcact
    25921 gcacaaatcg atcaatgggt tgcgcctgtc gcgctgtacc ccgattctct gctttcacaa
    25981 gtgttaatgg catcaaccta tccggcaaac gttgctcaag cagtgcaatg gtcgcacgat
    26041 aatccactta aacaaggcga tgctgctatt caggcggtat ctgaccagcc gtgggacgcc
    26101 agcgttaaat cactggtggc ctttccacaa ttgatggcat tgatgggcga aaacccgcaa
    26161 tgggtgcaaa acctgggcga tgcttttctg gcgcagccgc aggacgtgat ggactcggta
    26221 cagcgattgc ggcaactggc gcaacaaacc ggttcgctga agtcatcaac cgaacaaaaa
    26281 gttattacca caacgaagaa agctgtaccg gtaaaacaga cagtcacggc acccgtcata
    26341 ccatccaata ccgttttaac tgccagcccc gtcattacag agcctgcaac aaccgtcatt
    26401 tccattgagc ccgccaatcc tgatgtggtc tatattccca actacaaccc aaccgtggtt
    26461 tacgggaact gggccaatac tgcgtatccg ccggtttatc tgccaccacc agccggagaa
    26521 ccgtttattg acagctttgt gcgcggattc ggctatagca tgggtgttgc taccacgtac
    26581 gcactattca gcagcatcga ctgggatgac gacgatcatg accatcatca tcatgacgat
    26641 gatgattatc atcaccacga tggcggtcat cgtgacggta atggctggca acacaacggc
    26701 gacaacatca atatcgacgt caacaatttc aaccgtatca ccggtgagca tcttactgat
    26761 aagaatatgg catggcggca caatccaaac taccgtaatg gtgtgcccta tcatgatcag
    26821 gatatggcaa agcggtttca tcaaaccgat gtcaacggcg gaatgagcgc cacgcagcta
    26881 cctgctccaa cacgcgacag ccagcgtcag gcggcagcaa gtcagtttca gcaacgaaca
    26941 cacgccgccc ccgtcattac acgagatacc caacgtcagg cagcggcaca gcggtttaat
    27001 gaagctgaaa actatgggag ctatgacgac ttccgcgact tcagccgtcg ccaaccactg
    27061 acccagcaac aaaaggacgc cgctcgtcag cgttatcagt cggcctcgcc tgagcagcgc
    27121 caggcagtcc gcgagaaaat gcaaactaac ccacagatcc agcagcgaag agacgcagcg
    27181 cgtgagcgta ttcagtccgc ctcgcctgag cagcgccagg cagtccgcga gaaaatgcag
    27241 actaacccac agatccagca gcgaagagac gcagcgcgtg agcgtattca gtcagcctcg
    27301 cctgagcagc gccaggtgtt taaggaaaaa gtacagcagc gcccactgaa ccaacagcaa
    27361 cgtgataacg cccgccagcg tgttcaatca gcatcacctg aacaacgtca ggtttttcgg
    27421 gaaaaagttc aggagagctg cccacaacgt ctaaacgaca gtaaccatac tgtcaggctg
    27481 aataacgagc aacggtcagc agtacgcgaa cgtctctctg agcgcggagc aaggcgacag
    27541 gaaaggtaaa ttgcagacgt aaaaaaagcg gcgtggttag ccgctttttt aattgccgga
    27601 tgttccggca aacgaacaat tacttcttct tcgctttcgg gttcggcagg tcggtaatgc
    27661 taccttcgaa cacttctgcc gccaggccca cagactcgtg cagagtcggg tgcgcgtgga
    27721 tggtcagtgc gatgtcttca gcatcacaac ccatttcgat tgccaggccg atttcaccca
    27781 gcagctcgcc gccgttggta ccgacaatcg caccaccgat cacacggtga gattctttgt
    27841 cgaaaatcag cttggtcata ccgtctgcgc agtcggaagc gatagcacga ccagaagcag
    27901 cccacgggaa ggtggcggtt tcatagctga tgcctttctc tttcgcttct ttctcagtca
    27961 gacctaccca tgcaacttct ggttcggtat aggcgatgga cgggataact ttcggatcga
    28021 agtagtgttt cttaccggcg ataacttcag cggcaacgtg accttcgtga acacctttgt
    28081 gtgccagcat cggctgaccg acgatatcgc cgatagcaaa gatatgcggt acgttggtac
    28141 gcagctgttt gtcaacgcgg atgaaaccac ggtcgtccac ttccacgcca gctttgcctg
    28201 cgtcgaggtt tttaccgttc ggcacacgac caatcgctac cagcacggcg tcgtaacgct
    28261 gcggttcagc gggtgctttt ttgccttcca tcgtcacata aataccgtct tctttcgctt
    28321 caacggcggt aactttggtt tccagcatca ggttgaattt cttgctgata cgcttggtga
    28381 agactttaac gatgtctttg tcagctgccg ggataacctg gtcgaacatt tcaaccacgt
    28441 caatctgtga acccagcgcg tggtatacgg tgcccatttc cagaccgatg ataccgccac
    28501 ccattaccag caggcgttct ggtacttctt tcagttccag cgcgtcagtg gagtcccaga
    28561 tacgcggatc ttcatgcgga ataaacggca gttggatcgg gcgagaaccc gctgcaatga
    28621 tcgcattgtc gaagttgatc acggttttgc cgttctcacc ttcaacttcc agggtgttag
    28681 ccccggtgaa tttacccaga ccgttgacca ctttgacttt gcggcctttc gccataccag
    28741 ccagaccacc ggtcagctga ttgatcactt tctctttcca ggtacgaatc ttgtcaatat
    28801 cggttttcgg ttcgccgaag acgataccgt gttcagccag cgctttggct tcttcgataa
    28861 cttttgctac gtgcagcagt gctttagaag ggatacagcc gacgttcagg caaacaccgc
    28921 caagggtgtt gtaacgttct acgattacgg tttccagacc taaatcagcg caacggaagg
    28981 cagcggagta acctgcgggg cctgccccaa gtaccacgac ctgagttttg atttcagtac
    29041 tcatcatgac ctctatatat ttatctccgg cggtcatacc cgtcgtcttt caggccgcag
    29101 cggcgttagc ttcacgccct caccccagtc acttacttat gtaagctcct ggggattctg
    29161 gcgcttgcta ccttgctgca cccagaaatc catagggtat gtgaagtaat accctaacca
    29221 ccaccgggtc gttctatccg tcggtatttt acaaaattgt taacaatttt taaacaacaa
    29281 acggcaaccg atttgtctat tcgctaataa cctcaataca ttcatgagat taccagaaaa
    29341 aagccggccg ttgggccggc tcttttactt acatcaccag acggcgaatg tcagacagcg
    29401 tgttgttaat gatggtaatg aaacgggcac catcagcacc gtcgatcacg cggtggtcga
    29461 aggagagaga aatcggcagc atcagacgcg gcacgaactc tttaccattc cacaccggct
    29521 ccatcgcgga cttggaaacg ccgaggatag ccacttccgg cgcgttcaca atcggcgcga
    29581 agtgggtagt acccaggccg ccgatgctgg agatggtgaa gcaaccgccc tgcatttcgc
    29641 ccgcagtcag cttaccgtca cgcgctttct tagaaatagt catcagctcg cgagacagct
    29701 cgatgatgcc tttcttgttg acgtctttga ataccggaac aaccagaccg ttcggggtat
    29761 ccaccgccac accgatgttg atgtatttct tcagggtcag acgctgaccg tcttccgaca
    29821 acgaactgtt gaagcgaggc atctgctcaa gagctgcagc aacggctttc atgatgaaga
    29881 caaccggggt gatcttcaca tccagcttac gtttcgccgc ttcttcgttc tgctgtttac
    29941 ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg agtaacatgc gggatcatca
    30001 cccagttacg gctcaggttc gcaccagaaa ttttctggat acggcccagt tccacttctt
    30061 cgatttcacc aaacttgctg aagtccacct tcggccacgg cagcatgcct gggataccgc
    30121 cgccagtcgc cgccggagct gcttctgcac gtttgatagc ttctttcacg taagcctgaa
    30181 cgtcttcgcg caggatacga cctttacggc cagtgccctt cactttcgcc aggttaacgc
    30241 caaactcgcg tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg tcgttttctg
    30301 caaattcaga tttgccttcc gctttcgcag ccggtgctgc tgccggagct tcagcttttg
    30361 ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc cgccgcaggc gctgcgcctt
    30421 caacttcgaa gatcataatc agcgagccag ttttcacttt atcgccaacg ttgactttca
    30481 gttccttcac gacgcctgca aacggagccg gaacttccat agaagctttg tcgccttcta
    30541 cggtaatcag tgactgttca gcggcaactt tgtcgcccac tttcaccatc acttcggtca
    30601 cttcaacttt gtcaccgccg atatccggaa cgttaacttc tttcacgcca gccgctggtg
    30661 caggggccgc tgccggagcc gcttcctgtt tagccgccgg agctgccgcg cctgcttcac
    30721 ccgcgacttc gaagaccata atcagcgagc cggtagacac tttgtcaccc acgttcactt
    30781 tgatctcttt cacggtgcca gcaaacggag ccggaacttc catagacgct ttatcgcctt
    30841 ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc aactttcacc aggatttcgg
    30901 tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc gctgcagccg
    30961 ctggtgctgc tgccggagct gcttctttct tctcttctgc ctgagcaggt gcagcgtctg
    31021 ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt ttatcgccaa
    31081 cagagacttt gatctcttta acgacacccg cctgcggaga cggaacttcc atagaggctt
    31141 tgtcgccttc tacggtgatc agcgactgtt cggcttcaac tttgtcgccc actttgacca
    31201 ggatctcggt gatttcaact tcatcagccc cgatgtccgg tactttgatt tcgatagcca
    31261 ttattctttt acctcttacg ccagacgcgg gttaacttta tctgcatcga tgttgaattt
    31321 ggcgattgcg tcagcaacca ctttcttatc gatttcgcca cgtttagcca gttcgcccag
    31381 cgccgctaca accacgtagg aagcatcaac ttcgaagtgg tgacgcaggt tctcacggct
    31441 gtcggaacga ccgaagccat cagtacccag tacgcggtag tcgtcagccg gtacgtaagt
    31501 acggacctgc tcagcgaaca gtttcatata gtcggtagat gccactgccg gagcgtcgtt
    31561 catcacctga gcgatatacg gaacgcgcgg agtttccagc gggtgcagca tgttccagcg
    31621 ttcacaatcc tgaccatctc gcgccagttc ggtgaaggag gtcacgctat aaacgtcaga
    31681 acctacgccg taatctttcg ccaggatctc tgccgcttca cggacgtgac gcaggataga
    31741 accggagccc agcagctgaa ctttaccttt gctaccttca atggtttcga gtttgtagat
    31801 acctttacgg ataccttcct cagcaccttc cggcattgcc ggcatgtggt agttttcgtt
    31861 cagcgtggtg atgtagtagt aaacgttctc ttgtttttca ccgtacatac gctccagacc
    31921 gtcatgcata atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga tacagttcgg
    31981 aatagtcagc gactgaatgt ggctgtgacc atcttcgtgc tgcagacctt cgccgttcag
    32041 ggtggtacga ccggaagtac cgccgatcag gaagccacgc gcttgctggt cgccagccgc
    32101 ccagcacaga tcgccaatac gctggaagcc gaacatcgag taatagatgt agaatgggat
    32161 catcggcaga ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac aacctgcgcc
    32221 cagctcgttg atcccttcct gcagaatctg acctttctcg tcttctttat agtaagcaac
    32281 ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg ctgtaaatac caatctgacg
    32341 gaacagacct tccataccga aagtacgcgc ttcgtcggcg atgatcggta ccagacgatc
    32401 tttgatcgac ttgttcttca gcattacgtt cagggcacga acgaaagcga tagtggtaga
    32461 gatctctttg ctctgctctt ccagcagcgc gccgaagtct tgcaggctcg gcagctcaag
    32521 cttctcggtg aagttcggct gacggcttgg cagataaccg tgcagtttct gacgctgagc
    32581 gtgcagatag gtatgctctt cagaaccttc cgggaaggtg atgtacggca gtttttcgat
    32641 atctgcatca gacaccggca cattgaaacg gtcgcggatg tgacgcacgc cgtccatgtt
    32701 cattttctta acctggtgcg cgatgttttt accttcagcc gcgtcgccca tgccgtaacc
    32761 tttaatggta tgagcaagga ttactgtcgc tttgcctttg gtttcctgcg ctttcttgaa
    32821 tgcagcgtag attttcttcg gatcgtgacc accacggttc agtgcccaga tctgctcgtc
    32881 agtccagtct gcaaccagtg ctgcggtttc aggatattta ccgaagaagt gttcacgaac
    32941 gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg tcaacggttt cgttcatcag
    33001 ctggatcagt ttaccgctgg tatctttacg cagcagttca tcccaacggc taccccacat
    33061 cactttgatc acgttccagc cagcaccttc gaagatgcct tccagttcgt tgatgatctt
    33121 gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa cgaagaccag
    33181 gttatccagt ttttcacggg tagcgatggt gatcgcacct ttggattccg gttcgtccat
    33241 ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag gtatctttca ggccacggtg
    33301 ttccagatat ttcaggaatt tagcctggta aatagcacca atcggcccca gacccataga
    33361 tacggtcggg aactgccaga attccggcat cagtttcggg tgcggatagg aagagaggcc
    33421 attgccgtga acttcctgac ggaagttatc cagctgctcc tgagtcagac gaccttccag
    33481 gaaagcacga gcgtacacgc ccggggagat gtggccctgg aagtaaacca ggtcgccgcc
    33541 atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa tggttgcgga
    33601 agactggaag gacgccatat ggccgcccag ttcgaggtct tttttcgacg cacgcagcac
    33661 cgtcatgatg gcgttccagc ggatagctga acgaatacgg cgttccagtt ccagattacc
    33721 cggatactcc ggttgttctt caacggggat ggtgttgatg tagttgctga tacctgtgcc
    33781 tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc agttggtcga tcagatactg
    33841 agcacgctca acaccttctt cacggatgac cgattcgatc gcctggagcc agtcgcgagt
    33901 ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg ggttattcct tatctatcta
    33961 ataacgttga gttttctgga acctgtccca ttgaactctc gccggaaagc tcaataagac
    34021 aggttctacg tttagttgcc gcgcttttat atgcgcttga tttacaacat cttctggata
    34081 atttttacca gaaaaatcac taattctttc gttgctccag acgacgcaga gaacgctcac
    34141 ggcggctctc ttcacgactt ctgtcgagca aaatttcttc gataaaggcc agatggcgat
    34201 gcgatgcttc gcgcgcttct tccggcttac cggccataat cgcttcaaat atgcgggtgc
    34261 ggtgactact caccagcggc agcatctcgc gacgcgaata gagcaattcg aagttctggc
    34321 ggacattctg ggccaacatc ggctccatac accttagcag atgaagcaga accacattgt
    34381 gggccgcttc agtgacagca atctgatact ggagtacggc gtttgattcc gcgtccagat
    34441 cgccagactg ctgcgccagc tctatggcgt ggtggagttc acggatgcgt tccttgtctt
    34501 catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg tgtcgtgttt
    34561 cgagcaagtc atactgtgac tcaggatggt cggagagcag ctccaccagc ggatcgctga
    34621 agctttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga agcaacaagc
    34681 ccttcgcttc gagacgttga atcgcctcac gcaaggaggg acgggagacg tcaaactgtt
    34741 ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct tcgaggatca
    34801 aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt ttgctgtagg
    34861 ccatgagttc ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa tttcatgtct
    34921 gtgacgctaa agtaacaaag tattcacctt atgtccatac aggttttgat tgaaatcatg
    34981 aaactgtgca cattttaaca acttgacata tataacgttt caaagttgta actatgcaca
    35041 aatgtagact ttacgtagga aagga
//
LOCUS       DAZGTR010000037        34917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGTR010000037 DAZGTR010000000
VERSION     DAZGTR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.037"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(176..319)
                     /gene="ypaB"
                     /locus_tag="ACHLCY_002011"
     CDS             complement(176..319)
                     /gene="ypaB"
                     /locus_tag="ACHLCY_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP0369273.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            447..2732
                     /gene="nrdA"
                     /locus_tag="ACHLCY_002012"
     CDS             447..2732
                     /gene="nrdA"
                     /locus_tag="ACHLCY_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0369274.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2821..3951
                     /gene="nrdB"
                     /locus_tag="ACHLCY_002013"
     CDS             2821..3951
                     /gene="nrdB"
                     /locus_tag="ACHLCY_002013"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0369275.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3951..4205
                     /gene="yfaE"
                     /locus_tag="ACHLCY_002014"
     CDS             3951..4205
                     /gene="yfaE"
                     /locus_tag="ACHLCY_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP0369276.1"
                     /translation="MASVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4259..4909)
                     /gene="inaA"
                     /locus_tag="ACHLCY_002015"
     CDS             complement(4259..4909)
                     /gene="inaA"
                     /locus_tag="ACHLCY_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP0369277.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(4989..5093)
                     /locus_tag="ACHLCY_002016"
                     /pseudo
     CDS             complement(4989..5093)
                     /locus_tag="ACHLCY_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5091..5330
                     /locus_tag="ACHLCY_002017"
                     /pseudo
     CDS             5091..5330
                     /locus_tag="ACHLCY_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5372..6448)
                     /gene="glpQ"
                     /locus_tag="ACHLCY_002018"
     CDS             complement(5372..6448)
                     /gene="glpQ"
                     /locus_tag="ACHLCY_002018"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0369278.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6453..7811)
                     /gene="glpT"
                     /locus_tag="ACHLCY_002019"
     CDS             complement(6453..7811)
                     /gene="glpT"
                     /locus_tag="ACHLCY_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP0369279.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8084..9712
                     /gene="glpA"
                     /locus_tag="ACHLCY_002020"
     CDS             8084..9712
                     /gene="glpA"
                     /locus_tag="ACHLCY_002020"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP0369280.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9702..10961
                     /gene="glpB"
                     /locus_tag="ACHLCY_002021"
     CDS             9702..10961
                     /gene="glpB"
                     /locus_tag="ACHLCY_002021"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP0369281.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            10958..12148
                     /gene="glpC"
                     /locus_tag="ACHLCY_002022"
     CDS             10958..12148
                     /gene="glpC"
                     /locus_tag="ACHLCY_002022"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP0369282.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12342..13244
                     /gene="rpnE"
                     /locus_tag="ACHLCY_002023"
     CDS             12342..13244
                     /gene="rpnE"
                     /locus_tag="ACHLCY_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP0369283.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IEPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13285..13746)
                     /locus_tag="ACHLCY_002024"
                     /pseudo
     CDS             complement(13285..13746)
                     /locus_tag="ACHLCY_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(13810..15012)
                     /gene="yfaY"
                     /locus_tag="ACHLCY_002025"
     CDS             complement(13810..15012)
                     /gene="yfaY"
                     /locus_tag="ACHLCY_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP0369284.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            complement(15112..15654)
                     /gene="yfaZ"
                     /locus_tag="ACHLCY_002026"
     CDS             complement(15112..15654)
                     /gene="yfaZ"
                     /locus_tag="ACHLCY_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP0369285.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            15933..16358
                     /gene="nudI"
                     /locus_tag="ACHLCY_002027"
     CDS             15933..16358
                     /gene="nudI"
                     /locus_tag="ACHLCY_002027"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP0369286.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16397..16999)
                     /gene="ais"
                     /locus_tag="ACHLCY_002028"
     CDS             complement(16397..16999)
                     /gene="ais"
                     /locus_tag="ACHLCY_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP0369287.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17307..18446
                     /gene="arnB"
                     /locus_tag="ACHLCY_002029"
     CDS             17307..18446
                     /gene="arnB"
                     /locus_tag="ACHLCY_002029"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP0369288.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTTIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18450..19418
                     /gene="arnC"
                     /locus_tag="ACHLCY_002030"
     CDS             18450..19418
                     /gene="arnC"
                     /locus_tag="ACHLCY_002030"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP0369289.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19418..21401
                     /gene="arnA"
                     /locus_tag="ACHLCY_002031"
                     /pseudo
     CDS             19418..21401
                     /gene="arnA"
                     /locus_tag="ACHLCY_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21398..22288
                     /gene="arnD"
                     /locus_tag="ACHLCY_002032"
     CDS             21398..22288
                     /gene="arnD"
                     /locus_tag="ACHLCY_002032"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP0369290.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22288..23940
                     /gene="arnT"
                     /locus_tag="ACHLCY_002033"
     CDS             22288..23940
                     /gene="arnT"
                     /locus_tag="ACHLCY_002033"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP0369291.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            23937..24272
                     /gene="arnE"
                     /locus_tag="ACHLCY_002034"
     CDS             23937..24272
                     /gene="arnE"
                     /locus_tag="ACHLCY_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP0369292.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24272..24658
                     /gene="arnF"
                     /locus_tag="ACHLCY_002035"
     CDS             24272..24658
                     /gene="arnF"
                     /locus_tag="ACHLCY_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP0369293.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(24652..24918)
                     /gene="pmrD"
                     /locus_tag="ACHLCY_002036"
     CDS             complement(24652..24918)
                     /gene="pmrD"
                     /locus_tag="ACHLCY_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP0369294.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25028..26383)
                     /gene="menE"
                     /locus_tag="ACHLCY_002037"
     CDS             complement(25028..26383)
                     /gene="menE"
                     /locus_tag="ACHLCY_002037"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP0369295.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26380..27342)
                     /gene="menC"
                     /locus_tag="ACHLCY_002038"
     CDS             complement(26380..27342)
                     /gene="menC"
                     /locus_tag="ACHLCY_002038"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP0369296.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(27342..28199)
                     /gene="menB"
                     /locus_tag="ACHLCY_002039"
     CDS             complement(27342..28199)
                     /gene="menB"
                     /locus_tag="ACHLCY_002039"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP0369297.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28214..28972)
                     /gene="menH"
                     /locus_tag="ACHLCY_002040"
     CDS             complement(28214..28972)
                     /gene="menH"
                     /locus_tag="ACHLCY_002040"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP0369298.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDNQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(28969..30639)
                     /gene="menD"
                     /locus_tag="ACHLCY_002041"
     CDS             complement(28969..30639)
                     /gene="menD"
                     /locus_tag="ACHLCY_002041"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP0369299.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(30728..32023)
                     /gene="menF"
                     /locus_tag="ACHLCY_002042"
     CDS             complement(30728..32023)
                     /gene="menF"
                     /locus_tag="ACHLCY_002042"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP0369300.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32102..32407)
                     /gene="elaB"
                     /locus_tag="ACHLCY_002043"
     CDS             complement(32102..32407)
                     /gene="elaB"
                     /locus_tag="ACHLCY_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP0369301.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32462..32923)
                     /gene="elaA"
                     /locus_tag="ACHLCY_002044"
     CDS             complement(32462..32923)
                     /gene="elaA"
                     /locus_tag="ACHLCY_002044"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0369302.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            32988..33905
                     /gene="rbn"
                     /locus_tag="ACHLCY_002045"
     CDS             32988..33905
                     /gene="rbn"
                     /locus_tag="ACHLCY_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP0369303.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34093..>34917
                     /locus_tag="ACHLCY_002046"
     CDS             34093..>34917
                     /locus_tag="ACHLCY_002046"
                     /EC_number="3.4.19.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
                     /protein_id="HFP0369304.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFEKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFHINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLKRV"
BASE COUNT         8557 a         9342 c         8638 g         8380 t
ORIGIN      
        1 ataaccgaat agaaaacaac catttcgaca tcaacaatcg cttacattcg cttatatatt
       61 gaccacaact gatacatcag attatgtgat gactcgtgct tagatcaatt tttgcaatca
      121 ttagcaaaaa gattaataaa ccatctatat caatttatcc aacctattct gccgttcaag
      181 aaatcgccga acagttattt ttaacaaatt tttctcttcc cattgacttt cccggacacc
      241 ttgtctgacc taaggtgcgc gaaagccact ttttccttcc tgagttatcc acaaagttat
      301 gcacttgcaa gagggccatt ttcacactat cttgcagtga atcccaaaca taccccctat
      361 atatagtgtt ctaagcagct tcccgtacta caggtagtct gcatgaaact attgcggaaa
      421 gaattccaaa aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg
      481 gtagcacaga gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag
      541 gactgcataa cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg
      601 gtatcaagac ctctgacatc cacgaaacca ttatcaaggc ggccgcagac ctgatctccc
      661 gtgatgcgcc ggattatcag tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa
      721 aagcctacgg ccagtttgag ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga
      781 tgggcaaata cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg
      841 acacgtttat cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg
      901 aaggcaaata tctggtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc
      961 tttatattct ggttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat
     1021 atgtgaagcg tttttacgac gcggtttcca catttaaaat ttcgctgccg acgccaatca
     1081 tgtccggcgt gcgtaccccg actcgtcagt tcagctcctg cgtactgatc gagtgcggtg
     1141 acagtctgga ttccatcaac gctacctcca gcgcgattgt gaaatacgtt tcccagcgtg
     1201 ccgggatcgg catcaacgcc gggcgtattc gtgcgctggg tagcccgatt cgcggtggtg
     1261 aagcgttcca taccggctgt attccgttct acaaacattt ccagacagcg gtgaaatcct
     1321 gctctcaggg cggcgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg
     1381 aagtggaaag cctgctggtg ttgaaaaaca accgtggtgt ggaaggcaac cgcgtgcgcc
     1441 atatggacta cggggtacag atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag
     1501 atatcaccct gttcagcccg tccgacgtac cggggctgta cgacgcattc ttcgccgatc
     1561 aggaagagtt tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc
     1621 gtgtgaaagc cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta
     1681 tctatattca gaacgttgac cactgcaata cccatagccc gtttgatccg gccatcgcgc
     1741 cagtgcgtca gtctaacctg tgcctggaga tagccctgcc gaccaaaccg ctgaacgacg
     1801 tcaacgacga gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa
     1861 ttaatagcct ggatgaactg gaagagctgg caattctggc ggttcgtgca cttgacgcgc
     1921 tgctggatta tcaggattac ccgatcccgg ccgccaaacg tggagcgatg ggtcgtcgta
     1981 cgctgggtat tggtgtgatc aacttcgctt actacctggc gaagcacggt gtgcgttact
     2041 ccgacggcag cgccaacaac ctgacgcata aaaccttcga agcgattcag tattacctgc
     2101 tgaaagcgtc taacgagctg gcaaaagagc aaggcgcgtg cccgtggttt aacgaaacca
     2161 cttacgcgaa agggatcctg ccgatcgata cctataagaa agatctggat accatcgcta
     2221 atgagccgct gcattacgac tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc
     2281 gtaactccac gctttctgcc ctgatgccgt cagagacttc ttcacagatc tctaacgcca
     2341 ctaacggtat tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt
     2401 tgcgccaggt ggtgccggac tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa
     2461 tgccgggtaa cgatggctac ctgcaactgg taggtatcat gcagaaattt atcgatcagt
     2521 cgatctctgc taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc
     2581 agcagttgct gaaagacctg ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc
     2641 agaacacccg tgacggcgct gaagacgcgc aggacgatct ggtaccgtca atccaggacg
     2701 atggctgcga aagcggcgca tgtaagatct gataatcccc cagcctttgc accaaaccgt
     2761 cccctctccc cttgcgggag agggttaggg tgaggggtaa taaaaccaca ggacacactc
     2821 atggcatata ccaccttttc acagacgaaa aatgatcagc tcaaagaacc gatgttcttt
     2881 ggtcagccgg tcaacgtggc tcgctacgat cagcaaaaat atgacatctt cgaaaagctg
     2941 atcgaaaagc agctctcttt cttctggcgt ccggaagaag ttgacgtttc tcgcgaccgt
     3001 atcgattacc aggcactgcc tgaacacgaa aaacacatct ttatcagcaa cctgaaatat
     3061 cagacgctgc tggattccat tcagggccgt agcccgaacg tggcactgct gccgctgatc
     3121 tcgatcccgg aactggagac ctgggtcgaa acctgggcgt tctcagaaac gattcattcc
     3181 cgttcctata ctcatatcat tcgtaatatc gttaacgatc cgtctgttgt gtttgacgat
     3241 atcgtcacca acgagcagat ccagaaacgt gcggaaggga tctccagcta ttacgatgag
     3301 ctgatcgaaa tgaccagcta ctggcatctg ctgggcgaag gtacccacac cgttaacggt
     3361 aaaactgtga ccgttagcct gcgcgagctg aagaaaaaac tgtatctctg cctgatgagc
     3421 gttaacgcgc tggaagcgat tcgtttctac gtcagctttg cttgttcctt cgcatttgca
     3481 gaacgcgaat tgatggaagg caacgccaaa attattcgcc tgattgcccg cgacgaagcc
     3541 ctgcacctga ccggcaccca gcatatgctg aatctgctgc gcagcggcgc ggacgatcct
     3601 gagatggcgg aaattgccga agagtgtaag caggagtgct atgacctgtt tgttcaggca
     3661 gctcaacagg agaaagactg ggcggattat ctgttccgcg acggttcgat gattggtctg
     3721 aataaagaca ttctctgcca gtacgttgaa tacatcacca atatccgtat gcaggcagtc
     3781 ggtttggatc tgccgttcca gacgcgctcc aacccgatcc catggatcaa cacctggctg
     3841 gtgtctgata acgtgcaggt tgctccgcag gaagtggaag tcagttctta tctggtcggg
     3901 cagattgacg cggaagtgga caccgacgat ttgagtaact tccaactctg atggccagcg
     3961 ttaccctgcg catcactggc acacaactgc tgtgccagga tgaacaccct tcccttctgg
     4021 cggcgctgga atcccacaat gtggcggttg agtatcagtg tcgcgaaggt tactgcggct
     4081 cctgtcgcac ccgtctggtt gcaggtcagg ttgactggat tgccgaaccg ttagccttta
     4141 ttcagccggg ggaaattttg ccctgttgtt gccgggcaaa aggcgatatt gaaatcgaga
     4201 tgtgaattgg ttgtagtgcc agatacaacg cttatgcgtc ttatctggcc tacaacgatt
     4261 acatggcgta gtaatacgct ttcacctgct cccagtccgc tttggggatt ggctccagat
     4321 atttttccag ctggcggaag tcatgattaa tcgctttatc gcgacgcaag cggcgacgac
     4381 ttttctccag gtcaagaaaa ccagcttctg cattaccttc tgttttcaca tagatatggc
     4441 gaacataaca acagccatgc tgacgattaa tgctatgcat tttcttaaac gccagcgcca
     4501 ctgctttcaa catggcttgc cgtacttcgt cagaataagg cgatactgca tgctgggcat
     4561 accagtcagc aatgctgatg aaccccgcca tatcttcagt caccagcaac gctcgccatt
     4621 caccctcaat tttcaccgct tcaccaaaaa cgatcttcgg cacgatgaca ccggcccgtt
     4681 ccagttcttt aattaccgca acctcacgga caatcgttgg tcggccgaac ggataacgta
     4741 cggaatgaaa cagatgatgc gtcatgcgct ttacatacag ctttttgccg ttgcgctcga
     4801 cgcattgcac cccgctcata ccattacggc gatagttagg ctcttcaacc cagtcgccct
     4861 ctgttgccca ccagtgatta aattcgtcgt actttgctga aactgccata ccctatcgcc
     4921 tgtcattttt attaacgaca atgactatag gtggttacct gaggaaaatc ttaatgaaac
     4981 gtgtcgtatt aatgaatttc ttttgccact actagcgtga caccgctttt acccttcatt
     5041 tgcagcgcat agattccacc agccactacc actaatagtg cgcccagtag ataaaatgtg
     5101 aatttaatca gtcggataca gcgatgaagt ttattcgaca gggattaggc atcgccttac
     5161 aaccagagtt aacgctgaaa agcattgcag gtgaattgtg ttccgttcct cacgaaccaa
     5221 ccttctatcg acagatttcg ttgttggcta aagaaaagcc ggtagaaggc agtccactgt
     5281 ttttactaca aacgtgtacg gaacaactcg tggtgaatgg aaaaatttga tgtgagacag
     5341 gatggcgcat gaacgccatc ctgcagtagc attactcttt attaaggaat tttactgcct
     5401 tatcagggaa atcagtaaac agcccattta cacccgcttt gttatacaga gcatcatata
     5461 actgattcac atcagtagtg tattcaggca gtttatctga ccgcacggta taaggatgca
     5521 ctaccagttt attctgctga gcatcttgca ccatgccagt gagtttgata ttacccggct
     5581 gcgatgtttc ctcaatcaac atatggtaat ccggcccgat tccgtcggca tattccgcca
     5641 cctgtttcat agcacccggc ttaaacatcc agtcgtagtt gtaattaacc cagcttccat
     5701 ccggctgttt ctgctgcgtt tcattccagt cggtataggc aatcaactgt accagattga
     5761 gatccatgcc cattttaggt tccagctcat tcttaatacg cttcagctca tcagcatcaa
     5821 aacattgcaa ataaactttg tcgtccttac cggtgtaacc atatttcttc agcacttcca
     5881 gcgtttttgc cgcaatatcc ttcccttcct gatgatggaa ccacggcgct ttgatttcag
     5941 gataaatacc gatatttttc ccggtagagt gatttaaccc ctgaacaaat tcaatctctt
     6001 cttcaaaggt gtgcacccgg aagtcggact tacccattgg gaaacgcccc ggataagtct
     6061 gcactttttt accgttttca atatcgaaac cttcggtaaa tttcagcgac ttaatttcat
     6121 ccagcgtgaa atctatcgcg tagtaacgac cgtctttgcg cgcccggtcc gggaaacgat
     6181 cggcaacatc agtaacacga tcgagataat ggtcatgcag aacaaccaga tggtcgtctt
     6241 tggtcatcac caaatcctgt tccagataat ccgctccctg cgcatacgcc atcgcttttg
     6301 ctggcagcgt atgctccggc aaatatccac tggcaccgcg atgggcgatg actatttttt
     6361 cgttgctgtc cgccgccatt gcactgcttc ccatgactat agtgctcatc atgatcgcca
     6421 tgctaaggtt tttcagcgtc aatttcatgc cattagcctc cgttgcgttt ttgcagtaat
     6481 tgttcatggc gacgtttttc gccaatcatc acaacaatca acaagataac cgccagaatg
     6541 ctgccgccaa tcattaccat aaagccgcca tcccagccga agaagtccac ggtgtagcca
     6601 acaatcgcgc tcgccgccac cgaaccgccc aggtaaccaa acagcccggt aaagcccgct
     6661 gccgtacctg ccgctttttt cggtgccagt tccagcgcat gcagaccgat cagcatcaca
     6721 ggaccgtaga tcaggaagcc gataacaatc atacaaatca tatcgacggt tgggttacct
     6781 gccgggttca tccagtaaac gatagtcgcg atggtcacca gtgtcataaa gaaaacgccg
     6841 gttgccccac ggttgccacg gaagacttta tccgacatcc agccgcacag cagagtgccc
     6901 ggaatacctg catattcata aaggaagtag gcccaggagg atttatccag cgcgaaatgc
     6961 ttaacctctt tcagataagt cggtgaccag tcgaggatgc cgtaacgcag cagataaacg
     7021 aacacgttgg cgatggcgat ataccacagc agtttgttcg gcagcacgta ctgcatgaag
     7081 atttgcttcg cggtcagctc ctgttccgct ttttcgttat agtcgtccgg ataatcattt
     7141 ttgtactctt cgatcggcgg caagccacag gattgcgggg tatcgcgcat catcgcaaag
     7201 gcgaataatg ccaccagaat ggcgcagaaa gcaggcatat agagcgccgc atgccagtca
     7261 ttgaaccagg ccatccccag caggaacagc agcggcggaa taccaccacc gacgttgtgc
     7321 gcacagttcc acactgacac aatgccgcca cgttctttct gcgaccacca gtgcaccata
     7381 gtacgaccac acggcggcca ccccatcccc tggaaccaac cgcagaggaa caacagtaca
     7441 aacatcaccg caatgctcga cgtcgcccat ggcacaaagc ccataaacaa catcactgcc
     7501 gccgccagaa tcaaacctgc gggcaggaaa acgcgcggat tcgagcgatc cgataccgaa
     7561 cccattatga atttcgaaaa tccataagcg atcgagatcc ccgaaagcgc gaaacctaaa
     7621 tcaccgcgag agaatccctg ctcgaccaga taaggcatag caagcgcaaa gttcttacga
     7681 accaaatagt aagccgcata gccaaagaat atccccagga aaatttgcca gcgcaatcga
     7741 cgataagtcg gatcgatctc cgcagcaggt aagcgcgctt tgtgtggcgc tggtttaaaa
     7801 atactcaaca ttgaaagcct ccgtggcccg tggtcttatt tatgattaac agactgattc
     7861 agtgggagaa cctgccgttt ctttagttgc cgcgatgtta agaaaacatt cagaaattaa
     7921 atgtgaattg ccgcacacat tattaaataa gatttacaaa atgttcagaa tgacgcatga
     7981 aatcacgttt cactttcgaa ttatgagcga atatgcgcga aatcaaacaa ttcatgtttt
     8041 tactatggct aaatggtaaa aaacgaactt cagagggata acaatgaaaa ctcgcgactc
     8101 gcaatcaagt gacgtgatta tcattggtgg cggcgcaacg ggagccggga ttgcccgcga
     8161 ctgtgccctg cgcgggctgc gcgtgatttt ggttgagcgc cacgacatcg caaccggtgc
     8221 caccgggcgt aaccacggcc tgctgcacag cggtgcgcgc tatgcggtaa ccgatgcgga
     8281 atcggcccgc gaatgcatta gtgaaaacca gatcctgaaa cgcattgcac gtcactgcgt
     8341 tgaaccaacc aacggcctgt ttatcaccct gccggaagat gacctctcat tccaggccac
     8401 ttttattcgc gcctgcgaag aagcagggat cagcgcagaa gctatagacc cgcagcaagc
     8461 gcgcattatc gaacctgccg ttaacccggc actgattggc gcggtgaaag ttccggatgg
     8521 caccgttgat ccatttcgtc tgaccgcagc aaacatgctg gatgccaaag aacacggtgc
     8581 cgttatcctt accgctcatg aagtcacggg gctgattcgt gaaggcgcga cggtgtgcgg
     8641 tgttcgtgta cgtaaccatc tcaccggcga aactcaggcc cttcatgcac ctgtcgtggt
     8701 taatgccgct gggatctggg ggcaacacat tgccgaatat gccgatctgc gcattcgcat
     8761 gttcccggcg aaaggatcgc tgctgatcat ggatcaccgc attaaccagc atgtgatcaa
     8821 ccgctgccgt aaaccttccg acgccgatat tctggtgcct ggcgatacca tttcgctgat
     8881 tggcaccacc tctttacgta ttgattacaa cgagattgac gataatcgag tgacggcaga
     8941 agaggttgat attctgctgc gtgaagggga aaaactggcc cccgtgatgg cgaaaacgcg
     9001 cattttgcgg gcctattctg gcgtgcgccc gctggttgcc agcgatgacg acccgagcgg
     9061 acgtaacgtc agccgtggca tcgtgctgct cgaccatgct gaacgcgatg gtctggacgg
     9121 atttatcacc atcaccggtg gcaaactgat gacctatcgg ctgatggctg aatgggctac
     9181 cgacgcggta tgccgcaaac tgggcaacac gcgcccctgt acgactgccg atctggcact
     9241 gcctggttca caagaacccg ctgaagttac cttgcgtaaa gtcatctccc tgcctgcccc
     9301 gctgcgcggt tctgcggttt atcgtcatgg cgatcgcacg cctgcctggc tgagcgaagg
     9361 ccgtctgcat cgtagcctgg tatgtgagtg cgaagcggta actgcgggtg aagttcagta
     9421 cgcggtagaa aatttaaacg ttaatagcct gctggattta cgccgtcgta cccgtgtggg
     9481 gatgggtacc tgccagggcg aactctgcgc ctgccgcgct gccggactgc tgcaacgttt
     9541 taacgtcacg acgtctgcgc aatctatcga gcaactttcc accttcctta acgaacgctg
     9601 gaaaggcgtg caacccatcg cctggggaga tgcactgcgc gaaagcgaat ttacccgctg
     9661 ggtttatcag ggattgtgtg gtctggagaa ggagcagaaa gatgcgcttt gatactgtca
     9721 ttatgggcgg cggcctcgcc ggattactct gtggcctgca actgcaaaaa cacggcctgc
     9781 gctgtgccat tgtcacgcgt ggtcaaagcg cgctgcattt ctcatccggt tcgctggatt
     9841 tgctgagcca tctgcctgat ggtcaaccgg tgacagacat tcacagtgga ctggaatctt
     9901 tgcgtcagca ggcaccagcc catccttaca gtcttctcga accacaacgc gtgctcgatc
     9961 tcgcttgcca ggcgcaggca ttgatcgctg aaagcggtgc gcaattgcag ggcagcgtgg
    10021 aactggccca tcagcgcgtt accccgctcg gcactttgcg tgcaacctgg ttaagttcgc
    10081 cagaagttcc cgtctggccg ctacccgcga agaagatatg tgtagtgggg ataagcgggc
    10141 tgatggattt ccaggcgcat cttgcggcgg cttcgttgcg cgaactggat ctttcggttg
    10201 aaaccgcaga aatagagctg ccggaactgg atgtcctgcg caataacgcc accgaatttc
    10261 gcgcggtgaa tatcgcccgt ttccttgata atgaagaaaa ctggccgctg ttacttgatg
    10321 cgcttattcc tgtcgctaat acctgcgaaa tgatcctgat gcccgcctgc ttcggcctgg
    10381 ccgatgacaa actgtggcgt tggttgaatg aaaaactacc ttgttcactg atgcttttgc
    10441 caacgctgcc gccttccgtg ctgggcattc gtctgcaaaa ccagttacag cgccagtttg
    10501 tgcgccaggg tggcgtgtgg atgccgggcg atgaagtgaa aaaagtgacc tgtaaaaatg
    10561 gcgtagtgaa tgaaatctgg acccgcaatc acgccgatat tccgctacgt ccacgtttcg
    10621 cggttctcgc cagcggcagt ttctttagtg gcggactggt agcggaacgt aacggcattc
    10681 gcgagccgat tctcggcctt gatgtgctac aaaccgccac gcggggtgaa tggtataagg
    10741 gcgatttttt tgcgccgcaa ccgtggcagc agttcggtgt aaccactgat cagacgctac
    10801 gcccgtcgca ggcagggcaa accattgaga acctgtttgc catcggttcg gtgctgggcg
    10861 gatttgatcc catcgcccag ggatgcggcg gcggtgtttg tgccgtcagt gctttacatg
    10921 ccgctcaaca gattgcccaa cgcgcaggag gccaacaatg aacgacacca gcttcgaaaa
    10981 ctgcattaag tgcaccgtct gcaccaccgc ctgcccggtg agccgggtga atcccggtta
    11041 tccagggcca aaacaagccg ggccggatgg cgagcgtctg cgtttgaaag atggcgcact
    11101 gtatgacgag gcgctgaaat attgcatcaa ctgcaaacgt tgtgaagtcg cctgcccgtc
    11161 cgatgtgaag attggcgata ttatccagcg cgcgcgggca aaatatgaca ccacgcgccc
    11221 gtcgctgcgt aattttgtgt tgagtcatac cgacctgatg ggtagcgttt ccacgccgtt
    11281 cgcaccaatc gtcaacaccg ctacctcgct gaaaccggtg cggcagctgc ttgatgcggc
    11341 gttaaaaatc gatcatcgcc gcacgctacc gaaatactcc ttcggtacgt ttcgtcgctg
    11401 gtatcgcagc atagcggctc agcaagcaca atataaagac caggtcgctt tctttcacgg
    11461 ctgcttcgtt aactacaacc atccgcagtt aggtaaagat ttaattaaag tgctcaacgc
    11521 aatgggtacc ggtgtacaac tgctcagcaa agaaaaatgc tgcggcgtac cgctaatcgc
    11581 caacggcttt accgataaag cacgcaaaca ggcaattacg aatgtagagt cgatccgcga
    11641 agctgtggga gtaaaaggca ttccggtgat tgccacctcc tcaacctgta catttgccct
    11701 gcgcgacgaa tacccggaag tgctgaatgt cgacaacaaa ggcttgcgcg atcatatcga
    11761 actggcaacc cgctggctgt ggcgcaagct ggacgaaggc aaaacgttac cgctgaaacc
    11821 gctgccgctg aaagtggttt atcacactcc gtgccatatg gaaaaaatgg gctggacgct
    11881 ctacaccctg gagctgttgc gtaaaatccc ggggcttgag ttaacggtgc tggattccca
    11941 gtgctgcggt attgcgggta cttacggttt caaaaaagag aactacccca cctcacaagc
    12001 catcggtgcg ccactgttcc gccagataga agagagcggc gcggatctgg tcatcaccga
    12061 ctgcgaaacc tgcaaatggc agattgagat gtccacgagc cttcgctgcg aacatccgat
    12121 tacgctactg gcccaggcgc tggcttaacc gaccttttga tgcccggtaa gcacattgct
    12181 tatcgggcat ttttgcgagc gcgattccac gcaccatgta tgcgttgcaa cgctttgaaa
    12241 attcctctga aaatgtctcg caaaggctga aactgacaga tgtcaaaggc ccgggataga
    12301 cgtaatgtcg cgtcatcata aatatcaggt gacggacaac catgaccgaa tcaacaacat
    12361 cctccccgca tgatgcggtg tttaaaacct ttatgttcac acccgaaacc gcacgggatt
    12421 ttctcgaaat acatttacca gaaccactgc gcaagctttg caacctgcaa accttacgcc
    12481 tggaacccac tagttttatt gaaaaaagtt tacgcgctta ctactcggat gttttgtggt
    12541 ccgtggaaac cagcgacggt gacggctata tctactgcgt gattgaacat caaagctctg
    12601 cagaaaagaa tatggctttt cggctaatgc gctatgccac tgccgccatg cagcgtcacc
    12661 tggataaagg ctatgacaga gttccgctgg tggtgccatt gctgttttat catggcgaaa
    12721 catcgcccta cccgtactca cttaactggc tggatgagtt tgacgatccg caacttgccc
    12781 ggcagttgta caccgaagct tttccgttgg tggatatcac catcgaacct gacgatgaga
    12841 tcatgcaaca tcggcgtata gctctgctgg aactgattca aaagcatatt cgcgaccgcg
    12901 atttaatcgg catggtcgac aggatcacca cgcttttggt tagaggcttc actaatgaca
    12961 gccagctaca aacactgttt aattatctgc tgcaatgcgg cgatacctcc cgtttcaccc
    13021 gttttattga ggagattgcc gaacgttcac cactacaaaa ggagagatta atgactattg
    13081 ctgaacggct acggcaggaa gggcatcaaa ttggctggca ggaaggtatg catgaacaag
    13141 ccattaaaat tgctttgcgc atgctggaac agggcattga tcgtgaccag gtgctcgcgg
    13201 ccacccagct aagcgaagcc gatctggcag cgaataacca ctaattaaca caggcccaca
    13261 gccgatcccc atgggccttt gatatcaata actacctttt atgcgtggcc catttttgcc
    13321 tgatttaaac atcgccagtc gttgatccag ggcatcgctg tagagcatcg tgtcaacgcc
    13381 aacagcgaca aagttcgctc cccacgccag gcattgctgc gccatatcag gagccacagc
    13441 cagaaaacca gccgctttac ccgcagcacg gatccgccga atgctggttt caataattcg
    13501 ctgcacttcc gggtgcccgg cgttatccgg gtagcccaac gacgcagaaa gatccgctgg
    13561 tccaataaac acgccatcaa tcccttcgac gtcgaggatc tcgtccaggt tatccagtgc
    13621 cgttttactt tccacctgca ctaacagaca aagcgaatcg ttgacttgcg ccatgtaatt
    13681 ctcaatgcgt ccccagcgcg cagcccgtgc cacactggcc ccaacaccac gctcaccgta
    13741 gggagggttg gacaagtttt gccggagggg gaatgtgccg gtgcagtaaa ccggcacaga
    13801 gtggcggaat cagacagata aggtcatgga ctcaataacc tcaatccagc catgctcact
    13861 ggcgatatcc tggccgttta accaacggcg cagcatattc agtgccatca ttgcgcacac
    13921 ttcctgacgg atagccaggc tgtagcgcgt agtgctgaaa cgcacacgca gagcgaaagt
    13981 gccgtctggt gtggctagcg caaagttgag atgttcgttc tcgaaacccg aaacagccag
    14041 tgccagcccg gcaaaatggt tggcccgccg ttctgtaatc cagtgcgcag tttgcgccag
    14101 ggtttcttcc tgtgaaggaa ccacttcaca cgccagcaat ggagcacctg cgcgagaaag
    14161 ttgcaaagcc aataaaccac cggtgaattg ctcgctcaac gtcaggctga actggcggtt
    14221 ttgcaattcg cgactgatct gcgcgggcag tccttcggtg ccttcgaaaa tcacgctctg
    14281 tccggcaaca cgtttaacgt ccagccacag tttttccatc gcctgttgct cgcttgccgg
    14341 tccggtgagt ttcagttcga taatcggcat tgaagagcga tagcccattg ttacgcccgg
    14401 cggcagttgt agagtgtcca ggctttgtgc cagatcgctt tccgaacgac caaaggtagt
    14461 taaacgcaga caaaccggcg gctgtggtaa agaaaaacgc tcgcgcagac gcggcaggat
    14521 ttcgtgctcg accatcacct taaattctga cggtacgccg ggagtaaaga acatcaggca
    14581 acgattaagc tgcacggcaa aaccacaggc ggtgcctacc gggttattga taaattcagc
    14641 actggcaggc agctccgctt gtttacggtt gctcggtgcc attactcgtc cacgttcgtg
    14701 gaaatagcgt tccatctctt tgagccaggc ttcatgcagc accatgcctt caccttttgc
    14761 tgtcgcagcg gcgagtgcgc ttaaatcatc gctggtcggt cccagcccgc cgttaacgat
    14821 cagcacatcg gcgtgctgac tacgttcgcg aagaatggtg actaagtcat caaggttatc
    14881 ccccaccgta ttgcggcgag ataatggcag cccctgatga aagaaaaaat cggccagcca
    14941 ggcagcgtta gtgtcaacga tttgcccgtg taacacttca tccccggtgg atagcatttc
    15001 cacttttaac atggttatct cctgcttttg ttatcgaatc actataacgc aaagggagat
    15061 aaggcgtagt aagaaactga atggcgcgac agaatgccgc gccggaaggg atcagaaact
    15121 ggcgttaacc ccaacatacg ggccgtcagc cacggcgttg tcgcggttac cgtctttacc
    15181 cgacagattc aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg
    15241 cgcgccagca ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta
    15301 gtaatactcg ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac
    15361 cgctgcggca taaccttcat cgccgtaatt cgggttggtg taaacgcctt ttccgccaac
    15421 ggtcgccatt aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc
    15481 gtcgtcgtcg ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa
    15541 accgacacca atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac
    15601 cgatgcagaa accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc
    15661 ctttcccaca agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag
    15721 aggattcaat gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat
    15781 aattaaacgc cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc
    15841 agttatcatt gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc
    15901 atggtttctt tttatttata aggaataccc ctatgcgaca aaggactatt gtatgccctt
    15961 tgattcaaaa tgatggtgct tatttgctgt gtaaaatggc cgacgatcgc ggcgttttcc
    16021 ccggtcaatg ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac
    16081 tacgccgcga aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt
    16141 ggaccttcag cgatgatatt cgcaccaaga cgtatgcaga tggtcgcaag gaagagattt
    16201 atatgattta cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag
    16261 agtttcagga ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg
    16321 ccacccgaaa aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg
    16381 ctgtcagatt gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt
    16441 ccacatgcat gactaaacca tccagataat caggtttaaa tgtcgcatca cgcttatttt
    16501 tagcaatata tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt
    16561 tgctttgtaa gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat
    16621 ctaccgtcag ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat
    16681 tactggaata aagatcgaaa tcagggatgt cagcgctaaa agcgttgccc agttcacggg
    16741 catcctgggt acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac
    16801 gatcgcaacg ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg
    16861 ccaatgtttt attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca
    16921 gtccggcaat tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg
    16981 agcggcaaaa agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg
    17041 taaaattgag cgacatttct ggcgataatt agcaattggt gtaatattaa aaatcctacg
    17101 atgtcggcaa aatgcctcaa aattttgcca aatgcaaagc ctaaataaga aaaaatataa
    17161 aaatttcaat atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta
    17221 aaatttcgct ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca
    17281 ggtattcaat ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa
    17341 tgggcgtgga ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg
    17401 gtccgaagaa tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca
    17461 tcgcggtcag ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca
    17521 agggcgatga agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct
    17581 tgttgggtgc aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg
    17641 aagcgatcga gtcagccata acgccacgca ctaaagccat cattccggtg cattatgccg
    17701 gtgcgccagc agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta
    17761 tcgaagatgc tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag
    17821 gtaccactat tttttcattt catgccatta aaaatattac ctgtgcggaa ggtggcctga
    17881 ttgtaactga taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg
    17941 gtgtcgatgc ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac
    18001 cgggctataa gtacaatctg accgatatta acgccgcaat tgccctgaca cagttagcaa
    18061 aattagagca cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg
    18121 cagctctccc ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc
    18181 tgtttattat tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag
    18241 cgttaaaaga aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat
    18301 attatcgcga gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca
    18361 tctgttcttt gccgctgttc ccggatatga ctaccgccga tgccgaccgt gtcatcacag
    18421 cccttcagca actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct
    18481 cggtggttat tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca
    18541 ccacagcctg tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta
    18601 gcgataattc cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt
    18661 ctattttgct taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg
    18721 ttaccggcga cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc
    18781 cccgcctggt ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc
    18841 gccaggacag ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca
    18901 ccaccggcaa agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg
    18961 tcgatgcgat gttgcactgc catgaacgta gcacctttat cccgattctg gcgaatatct
    19021 tcgcccgccg tgccattgaa attccagtac atcattccga gcgtgagttt ggtgaatcca
    19081 aatacagttt tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa
    19141 cgccgctacg tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg
    19201 cggtgctgct ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct
    19261 ttatgctatt tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac
    19321 tcggtgaata tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc
    19381 agcaagttat ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta
    19441 ccacgatatg ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc
    19501 catttttacc cataccgata atcccggtga aaaagccttt tatggttcgg tggctcgtct
    19561 ggcagcggaa agaggcattc cggtttatgc gccggatgac gttaatcatc cgctgtgggt
    19621 ggaacgcatt gcccaactgt cgccagatgt gattttctct ttttattatc gccatcttat
    19681 ttgcgacgaa attttgcagc tcgctcccgc aggtgcattt aatctgcatg gttcgctgtt
    19741 accaaaatat cgtggtcgcg cgccgctgaa ctgggtgctg gtcaacggtg aaacggaaac
    19801 tggcgttaca ttgcaccgaa tggtgaaacg tgccgatgcc ggggccattg tggcgcaact
    19861 gcgcattgcc attgcgccag acgatatcgc tattacgctg catcataaat tgtgccatgc
    19921 cgcgcgccag ctactggaac agacattacc cgccattaaa cacggtaata ttctggaaat
    19981 cgcccagcgc gaaaacgaag ccacctgttt tggtcgcaga acgccggatg acagtttcct
    20041 tgaatggcat aaaccggcat ccgtactgca caacatggta cgtgccgttg ccgatccgtg
    20101 gccgggtgcc ttcagctatg ttggcaatca gaaattcacc gtctggtcgt cgcgtgttca
    20161 tcctcatgcc agcaaagcac aagccgggga gcgtgatttc tgttgcgcca ctgctgattg
    20221 cctgtggcga tggcgcgctg gaaatcgtca ccggacaggc gggcgacggc attactatgc
    20281 agggctcgca attagcgcag acgctgggcc tggtgcaagg ttcacgcttg aatagccagc
    20341 ctgcctgcac cgcccgacgc cgtacccggg tactcatcct cggggtgaat ggctttattg
    20401 gcaaccatct gacagaacgc ctgctgcgcg aagatcatta tgaagtttac ggtctggata
    20461 ttggcagtga tgcgataagc cgttttctga atcatccgca ttttcacttt gttgaaggcg
    20521 atatcagtat tcattccgaa tggattgagt atcacgtcaa aaaatgtgat gtcgtcttgc
    20581 cgctggtggc gatagccacg ccgattgaat atacccgcaa cccgctgcgc gtatttgaac
    20641 tcgattttga agagaatctg cgcattatcc gctactgcgt gaagtaccgt aagcgaatca
    20701 tcttcccgtc aacttcagaa gtttatggga tgtgtagcga taaatacttc gatgaggacc
    20761 attctaattt aatcgtcggc ccggtgaata aaccacgctg gatttattcg gtatcaaaac
    20821 aattacttga tcgggtgatc tgggcctatg gcgaaaaaga gggtttacag ttcaccctct
    20881 tccgcccgtt taactggatg ggaccgcgac tggataacct taatgcggca cgaatcggca
    20941 gctcccgcgc tattacgcaa ctcattctca atctggtaga aggttcaccg attaagctga
    21001 ttgatggcgg aaaacaaaaa cgctgcttta ctgatattcg cgatggtatc gaggcgttat
    21061 accgcattat cgaaaatgcg ggaaatcgct gcgacggtga aattatcaac attggcaatc
    21121 ctgagaacga agcgagcatt gaggaactgg gcgagatgct gctggcgagc ttcgaaaaac
    21181 atccgctgcg ccatcatttc ccaccgtttg cgggctttcg cgttgtcgaa agtagcagct
    21241 actacggcaa aggatatcag gacgtagagc atcgtaaacc gagcatccgc aatgcccacc
    21301 gctgcctgga ctgggagccg aaaattgata tgcaggaaac catcgacgaa acgctggatt
    21361 tcttcctgcg caccgttgat cttacggata aaccatcatg accaaagtag gcttacgcat
    21421 tgatgtcgat acctttcgtg gcacccgtga aggcgtgccg cgtctgctgg aaatcttgag
    21481 taagcataat attcaggcca gcattttttt cagcgtcggc ccggacaata tgggccgcca
    21541 tctctggcga ctggtgaagc cacagttttt gtggaagatg ctgcgctcaa atgcggcatc
    21601 gctttatggc tgggatattt tactggcagg tacggcctgg ccaggtaaag agattggtca
    21661 tgccaatgcc gatatcattc gtgaagcggc taaacatcac gaagtcggcc tgcacgcctg
    21721 ggatcaccat gcctggcaag cccgtagcgg taactgggat cggcaaacaa tgatcgacga
    21781 tattgcacgc ggtcttcgca ctctggaaga gattatcggt caaccggtaa cctgttctgc
    21841 cgctgcgggc tggcgtgccg accagaaggt gatcgaagca aaagaagcgt tccatttgcg
    21901 ctacaacagc gattgtcgtg gggccatgcc gttccgtcca ttgctcgaat caggaaaccc
    21961 tggcactgcg caaattccgg tgaccttacc cacctgggat gaagtgattg gtcgggatgt
    22021 gaaagcagaa gattttaacg gttggttact caaccgcatc ctgcgagata aaggcacgcc
    22081 ggtttatacc attcatgcag aagttgaagg ctgcgcttat cagcataatt ttgtggatct
    22141 cctcaaacgc gcagctcagg aaggcgtgac attttgccct ttaagcgaac tgttatcaga
    22201 gacgttgccg ctcggacaag ttgttcgcgg aaatattgcc ggacgtgaag gctggctggg
    22261 ttgccaacaa attgcgggta gtcgctgatg aaatcggtac gttaccttat cggcctcttc
    22321 gcgtttattg cctgctatta cctgttaccg atcagcacgc gtctgctctg gcaacccgat
    22381 gaaacgcgtt atgcggaaat cagtcgagaa atgctggcat ccggcgactg gattgtgccc
    22441 catctgttag ggctacgtta tttcgaaaaa cccattgccg gatactggat taacagcatt
    22501 gggcaatggc tatttggcgc gaataacttt ggtgtgcggg caggcgttat ctttgcgacc
    22561 ctgttaactg ccgcgctggt gacctggttt actctgcgct tatggcgcga taaacgtctg
    22621 gctctactcg ccacagtaat ttatctctca ttgtttattg tctatgccat cggcacttat
    22681 gccgtgctcg atccgtttat tgcattctgg ctggtggcgg gaatgtgcag cttctggctg
    22741 gcaatgcagg cacagacgtg gaaaggcaaa agcgcaggat ttttactgct gggaatcacc
    22801 tgcggcatgg gggtgatgac caaaggtttt ctcgcccttg ccgtgccggt attaagcgtg
    22861 ctgccatggg tagcgacgca aaaacgctgg aaagatctct ttatttacgg ctggctggcg
    22921 gttatcagtt gcgtactgac ggttctccct tggggactgg cgatagcgca gcgggagcct
    22981 gacttctggc actatttttt ctgggttgag catattcaac gctttgcact ggatgatgcc
    23041 caacatagag ctccgttctg gtactacgtg ccggtcatca ttgccggtag cctgccgtgg
    23101 ctgggattac tccccggtgc actgtacaca ggctggaaaa accgcaagca ttccgcaacc
    23161 gtctatttgt tgagctggac gataatgccg ctgctgtttt tctccgtcgc taaaggtaaa
    23221 ttgcccacct atattctttc ctgctttgca tctctggcaa tgctgatggc gcattacgct
    23281 ttgctggcag caaaaaataa tcctctggcg ctgcggatta atggctggat taacatcgct
    23341 tttggcgtca ctggcattat tgccacattt gtggtctccc cgtggggacc aatgaacacg
    23401 ccggtgtggc aaaccttcga gagctataaa gtcttttgtg cctggtcgat tttttcgcta
    23461 tgggcatttt tcggctggta caccttaaca aacgtcgaaa agacctggcc ttttgccgcg
    23521 ctttgcccgc tggggctggc gttgctggta ggattttcaa ttcctgacag agttatggaa
    23581 ggaaaacatc cgcaattttt tgtcgagatg acacaagaat cacttcagcc aagccgctat
    23641 attcttactg atagcgtcgg tgttgccgca ggtctggcat ggagcctgca acgcgatgac
    23701 atcatcatgt atcgccagac aggtgagttg aaatacggcc ttaattatcc ggatgcgaaa
    23761 gggagatttg tcagcggtga tgagttcgca aactggctta atcaacatcg tcaggagggg
    23821 attattactc tcgtgctttc ggttgaccgc gatgaagata tcaacagtct cgccattccg
    23881 cccgcagatg ccatcgatcg tcaggagcgt ctggtgctga ttcagtatcg tcccaaatga
    23941 tctggctaac attagtcttt gccagcttgc ttagcgttgc cgggcagttg tgtcagaaac
    24001 aggcaacctg ctttgtggcg ataaacaaac ggcgcaaaca tatcgtgctg tggctgggac
    24061 tggcgctggc ttgtcttggt cttgccatgg tgctctggct gctggtcttg cagaacgtac
    24121 cggtaggcat tgcctacccg atgttaagtc tgaattttgt ctgggtgacg ctggctgcag
    24181 taaaactgtg gcacgaaccg gtatcgccgc gtcactggtg tggggtggcg ttcattattg
    24241 gtggcattgt gatcctcggg agtacggtgt aatgggcctg atatggggat tattcagcgt
    24301 cattattgcc tcagttgcgc aattaagcct gggttttgcg gcaagtcatc tgccgccgat
    24361 gacgcactta tgggatttta tcgcggctct gttagctttt ggcctggatg cccggatact
    24421 gttactcggg ctgctgggtt atctgctttc cgttttttgt tggtataaaa cgctacataa
    24481 acttgcgcta agcaaagcct atgccttgct cagtatgagt tatgtgctgg tatggattgc
    24541 ttcaatggta cttcccggct gggaagggac tttttcgctg aaagcactac tgggagtagc
    24601 ctgtattatg agcgggttga tgctgatttt cctgcccacg acaaaacaac gttactgagt
    24661 tttccctgcc gctgtacact gccgttccca ctcatcgggg ctataactgc ttgccgagag
    24721 gactttcacc gtatgcagct tttcacgatt aatacaataa agcgcattct gcaaaggcga
    24781 gagtaaatcc cccactttca cggcgaagtc actcttcact tcggcgatca tttttatcgc
    24841 gccgccagca tcgcagagca taagtacatg tctgttatct tgtttattgc agcacgattt
    24901 tttgaccagc cattccattg cattatcctg tttgctaaga gttttcacat caatttacag
    24961 attaacagat aaactcttta tcaacggcaa tggaatggca gaaatgagaa gtgaaacgga
    25021 gggttaatta ttgctgacgt tgcacccact cttttagcgc ctgacgtgaa attttaatac
    25081 cgccgttttt cagctccggc ggtaaagtca gccagcgcac cggttgttga aaacgagcca
    25141 gcttatcttt cacccattcg ccaagatcaa cggtttgctg gtcatactcc accaccgcta
    25201 ccggccgatg accaaactcc ttgtcggcaa cgggaacgat aaacacctgc aaaaccgcag
    25261 gatgtgcagc aataacacgc tcgacttctt ccggctgaat accctctccg ccactgaaga
    25321 atagattgtc taaacgtccg acaatggtca gcttgccatt atgcatctca ccgcgatcgc
    25381 gcgtagcgta ccagccttcg tcattaacca gtgaaaccag ttgcccgtta cgccagtaac
    25441 cttctgccat actggcagcc cgcagccaca cttcattatt aacgattttc acttcccgac
    25501 ccggcagcgg cgaaccaacg tctgccaggc cgtcggcttc tttcgcacac accgtggagg
    25561 caaactcggt cagaccatag ccgcaaaagc aacgaatccc ctgctcgcgc gcctgttccg
    25621 tcaactcgac cgggatagcc gcgccgccaa gtaacaccgc tttcagggaa acggaactgc
    25681 ggttaaccag caaacgccag agttgtgttg gcaccagtga agcatgagta cagcctgcca
    25741 gcatttgctc caatggctgt ttatcacgca ccgtcatccg cgcaccagcg tataaccagc
    25801 gccacataat tccctgaccg gagacgtgaa acagcggtaa agagagcaac cagtcatcgt
    25861 gatcgccaaa cggaatcaac gataacaccc cttcggcact ggcaagatgg gcctgataag
    25921 tatggacagc ggctttcggc aacccggtag aacccgaggt caacgtcatt gagcacagac
    25981 gcgtcggctg ccacgtagcg gcatgtgcgc cttcaacccg ctgaatgtgc agcgacgtta
    26041 acgccggaaa cgtgttttcc ccatccggca ccagcgcaaa ttgcagcgtc agattgggca
    26101 gcaattcttc aagcaacggt tgcggcagct gagggttcac gggcaacacc cgcgccccgc
    26161 attgcagtaa cgccagccag gcgagcagcg tttgcggcgt attccacgcc cgcaacatca
    26221 cgccgctgcc ctcaacaacg ccctgcgccg caaagcctga ggctaattca tcgacgcgag
    26281 cacaaagctc gcgccagtta agttgctcgt cattaagacg taaggcgatg gcttctcccc
    26341 gcacttgccg ccagtgacgc cacggccagt cagagaagat cataacaacc gctccagtgc
    26401 atcaacttcc acgacaggca gcgtgctacc cggccagcga cgtacctgct gcgcctgcat
    26461 cagatccagc gtgtccagcc ctggaatggt gtccggcgtt aaccaggcgg caatccgcgc
    26521 cagttgcgtt aagcctaagc tcgattcaat ggaagaactg atcaccgccg tcagccccag
    26581 cgcgtgcgcc gcctgtacct gctcgcgtac tttctccaga ctgcccgtga gcgtgggttt
    26641 gataactacc gcgcgcacgc cctcttcagc cacaaaggca aaatccggct cgcgcaagct
    26701 ttcatcccag gcaatggcaa tgccggtttc acgggcaaac gctcgcgaat catcgcgggt
    26761 tttgcacggc tcttcgagaa acgcgatgcg gtggcgataa tccgggttaa catatttggc
    26821 aaactgctgg cctttcagcg gtgtccaggc gcgatttgcg tcaagacgca aatgcagatc
    26881 cggaattgcc tccagcaaca gattaaccac catgccgtcg cgcacggctt cgtacaatcc
    26941 cactttgacc ttcgccactt tctcgcctgg catatcagca agtttgagga tcagatcgtc
    27001 aggatcgcca ttacacagtg gtgccgcacg gtaattggcc gcctgcggga gcgtatctgc
    27061 cagctccgcc aatgcacagc ttacgccaaa ggcaacggaa ggcatctgcg gtagctcgca
    27121 atcgcctgcc agccagttat ttacccaggc aagcagcacg ctttgcgcct cttcccaggt
    27181 ttcctggctg aagcccggca gtggggagat ctccccccac ccttcgcgct cgccctcacg
    27241 caggcaaacg tacagcccgt cgcgggtttt taaccgcctg tcgcgcagaa ccacccccgc
    27301 gtccatgggg atctgccagc ggtatacctg cgcgctacgc attacggatt ccgtttaaat
    27361 ttgctgaaat caggctgacg tttctggttg aaagcgttgc gaccttcctg accttcttcc
    27421 gtcatgtaga acagcatggt ggcgttgccc gccagctcct gcagccccgc ctgcccgtca
    27481 cagtcggcgt tcagcgcagc tttcaggcag cgcagcgcca tcgggctgtt ttgcagcatt
    27541 tcgcggcacc aacggacggt ttctttttcc agatccgcca gcggtaccac ggtgttgaca
    27601 aggcccatat ccagcgcctg ttttgcgtcg tactgacggc acaggaacca gatttctcgc
    27661 gcttttttct gcccgacgat acgagccatg taggaagcgc cccagccgcc gtcgaaggaa
    27721 ccgactttcg ggccagtctg accgaagatg gcattatctg ctgcgatagt caggtcgcac
    27781 atcatgtgca gaacgtgacc accgccgatg gagtagccag ccaccatcgc gacaaccggt
    27841 ttcggacagg tacggatctg acgctggaag tccagcacgt tcaagtgatg tacgccggaa
    27901 tcatctttat agccgccgta atcaccacgc actttctggt caccaccgga gcagaacgct
    27961 ttatcgcctg cgccagtcag aatgatcacg ccgatattgt cgtcataacg cgcatctgcc
    28021 agtgcctgga tcatctcttt taccgtcaga ggacggaagg cattgcgcac ctgcggacga
    28081 ttaatggtga tttttgcgat accgtcggtg gatttttcat aacgaatgtc ctcgaaacct
    28141 tcggagcagt cgtgccattc aaccggtgcg taaagcattg cttcatcagg ataaatcata
    28201 ttgtgtcctt tagtcagaaa cgcaagatct gcgccagact tgcgattacg ccagcgggat
    28261 tttcccgatg cgcgttatgt ccggcgcgag gaatgacatg gcagtcggca gccagttccg
    28321 ccgccagggc gcggaatttg ctgtcacgtt caccacataa ataataaaac gcaaatgtgc
    28381 gggcgctaag gttagcacgt aaatcaggct ggacggcgag agaagtcgcc tccagcatgg
    28441 cggcaagggt tgcgccatta ttgttgctgc gcagcgccac cagctcccgg cgttgattgt
    28501 cattgagcga ggcaaaaaca ggctgttgat accagtcggc aaataccgcc gttaacggtt
    28561 ctgtgcaaaa acgctgcgcc cattggcgat cggaacgctg acgttccgca cgttgttcag
    28621 cattttgcag ccccggatgc ccgccttcga caataacccc acaaagcccc gccagcccct
    28681 ggcaagccgc catcatcgcc acccgtccgc caagcgagta ccccaccagc cagaagtcaa
    28741 ggatgttgta actaaccaag gttttacgca gtaagtcggt gacatcatca aatccatcga
    28801 cgctaatcgc cgccgaacca ccatgacctg ggagatcaac atacaaccgt gagtagtcgg
    28861 caaacgcctc gcccacttct tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca
    28921 ccagccaggg taaacctggt tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct
    28981 tacctgcgcc agaagttgct ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc
    29041 aatcaccgtg gtggttggcg tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca
    29101 gttttgcgga cgatgatatt tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt
    29161 ttgcggcatc agatagaaac gctcacgctc gctttgcggc gttggcaaca gcgagaaaat
    29221 ttgcccgccg ttgttgttca ccacaattaa taccagcggc gcagaaacct gacgcaataa
    29281 cgccagcgcg ttgagatcgt aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt
    29341 gccgcttgcc cgctgaacgc ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc
    29401 tcggttgctg tacaccgggt aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac
    29461 caccaggctg ttaccaacaa acaattgccc ctgttcaggc agatagtcgc tgatgcgatg
    29521 cgccagttgc gcttcgccaa acgcatcacg gcgggcaata accgcctgca ttgcctgttc
    29581 cgccaggcgc gggatttcaa cgcaccaggg ctggcgtttt tctgccggat gcagctccag
    29641 ccagtcggca atattggcaa ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc
    29701 ttcaatgtca tcaacaatcc agtactcttc gggttcacag cttgcctgcc attgcaggag
    29761 ccgtttcccc gtcaggctgc ttcccagttg caccacaatt tgcgcctgct gcagctcgct
    29821 ggtcgctttg gcattgccta accaaagatc ggcacacggc agcggctgcc cggtttgtga
    29881 cagcacatcg ccaatcagcg gccagccaag agtttgcgcc cacagggcaa cttttttgcc
    29941 ctcttccgca ctcatgcgcc cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca
    30001 gtcgcgctgt ttttcacttt ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg
    30061 ccaccagtca cccagacgct gttgccagct aagcccggta tcgtccattt cgccatacag
    30121 cggttcagca aacgggcagt tgatatggac tccccccgca tgaagcgtac cgagagcgtg
    30181 gtcgatggta gaaaccagcc aacgtgcggg gatatcctgg gtcgggcgcg gcaatgaaat
    30241 actgtgcgtg gggtgagagg cgaacattcc cggctggcga attgcctgat tcgcgccgca
    30301 gtcaattagc tccggcgggc gatcggcggt taagagaatc agtttttctc cggttaaccc
    30361 ggcttcaatc agtgccggat agagatttgc caccgccgtg ccggaggtca caatcaccgc
    30421 caccggctgc ttgctgactt tcgccagccc cagcgccaga tgccccagtc cacgctcatc
    30481 gaaatgggtg tggtgaatga atgcggaatt ctccgccgcc gctaacgtta acggtgtaga
    30541 acgcgagcct ggggcgatac agatgtgtct gacgccgtga cgcgttaatg cttccagaat
    30601 gaccgccgcc cagcgtcggt taaatgcgct tactgacatg agtttgtccg gtatcaatat
    30661 tgcggctaag tataaggagc tacaaaaata gattattgat atgaatcggt aatgatgcga
    30721 ctcattacta ttccatttgt aataaagtac gcagccctgc cgctttgttg tcgatttcct
    30781 gccactcttg ctcggggtcg gaaccacgga caatgcccgc gccagcatat agccgcacga
    30841 cattgccgct gatttttgct gaacgcaggg aaacgcagaa ttcgctttgt tgtaatgaga
    30901 gatagcccgc agaaccggcg taccattctc gggtgaacgg ttcgtgacgg gcgataaact
    30961 gtcgcgccag atcgcgcggt aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac
    31021 agatcacatc atccgctttg ttgagtgaag tccagataca gcggcgaaga tgctgcactt
    31081 tacgcagacg cagtacctgc ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc
    31141 gttggcagat atcttccacc accagcatgt tctcgcgctg gtttttatca tccgccatca
    31201 gccactctcc taactgctgc gcctgcttat catcaggatt atttgctact gttcccgcca
    31261 gcgcttcagt acgcagcgct ttgtcacgcc gccgccataa ccgttccggt gaagagccaa
    31321 gaaaagcatt ttcgccatca aaggccatgt aaaaatggta gcaattcaga ttcagtcgac
    31381 gactggcagc catcatcgcc gccgcgttga ccggacttgc gaaatgcagg tcagttgccc
    31441 gagcgagcac cactttgtcg agctcacctt cggcgatggt tttcgttgcc agttcgatta
    31501 attgcgtcca gcccgttttg tccggccagt gttgttctcg cgtggtggtt aaatgtaacc
    31561 caggcaaggg cttgatactc accagtgtgg cgataaattc ttttgcctga atcgcatcgt
    31621 gctgaaggga gctttcgctg aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc
    31681 gccattccag gcggggtaaa agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc
    31741 aaatgcgtaa gtcggcgtgt tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg
    31801 acgtaaaacg ggtaatcgcg cccaggacgg cagcttcttc atcaccatta cgttgttgcc
    31861 agtagaattg cgggtacgtt tgctgactgg ccagccagct caaggcatca aaagcgtctt
    31921 tgagagggaa aggaatatcg ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca
    31981 aatggcgcag tagattttcc agcgccgtag taagtgattg cacgcggacc tctccctgtt
    32041 aaaagcctca cattatacgg ggtactacaa aaaaaatgca gtaccccggt gtagggaggt
    32101 tttaacggcg tgccagcaac agtcctagta ccagcccaac ggccgcgccc acaccaattc
    32161 cttgccaggg tttttcgtgg acgtagtcat cagcacgata aactgcctgc ttcgcccgat
    32221 agtaataact gtctgaagcc tggctaaccc gttttttcac atcatccagc gctttttctg
    32281 cacgcgcttt cagctcaaca tatttctgat cggcgggatc gccagaggag cggagcacct
    32341 cttccagtgt ttcactaagc agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat
    32401 tagacattct cgttctccat ttgcgtaaaa cctgtcacta actatagaca agggttgttg
    32461 attacgcctg aattacctcg cgtgccatcc caatgtgtgg gataccatct tcttcataga
    32521 cctccgtcac agggataaaa ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc
    32581 cgaggtaaac aggcttatca ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca
    32641 gctgctgccc cactttttcg ccacgcaacg cttcactgac aatcacccga cctataacga
    32701 ccggctcaag atcatcatca cttttcagaa tcctcgcata tgccaccagt tcatcatttt
    32761 tccaaccgag aatatggcga ttatcccccg tcaaatcatc accatcgata tcctgataag
    32821 ggcaattctg ctcgacaaca aacaccgcgc aacgcagttg taataaggca taaagctggg
    32881 aaaccgataa ttcagagtga tgcagatctt gccattcaat cataatgctc tccttttaga
    32941 tgtatgcctt atactagccg gaatattttt tgaaacgtga tgaactcatg gaattaattt
    33001 ttttaggtac ttcggccggt gtgccaaccc gcacgcgcaa tgtcacggca atattgctta
    33061 acctgcaaca tccgacccag agcggtcttt ggttgtttga ctgcggtgaa ggcacccagc
    33121 atcagctact gcataccgcc tttaaccctg gaaaactgga caagattttt atcagtcacc
    33181 ttcatggcga tcatcttttt ggtttacccg gcttgctgtg cagtcgttct atgtcaggca
    33241 ttatccaacc cttaacgatt tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc
    33301 tgcggattag cggctcatgg accgattatc cgctggaaat tgtcgaaatt ggcgctggcg
    33361 aaattctcga tgatggcctg cgcaaagtaa ccgcttatcc gctggaacac ccactggaat
    33421 gttatggcta tcgtattgaa gaacatgata aaccgggtgc attaaatgcc caggcattaa
    33481 aagctgctgg cgtgccgcct ggcccactgt ttcaggaatt aaaagcgggc aaaacaatca
    33541 cgctggaaga tggaaggcag attaacggcg cagattactt agctgctcca gtgccaggta
    33601 aagcgctcgc tattttcggc gataccggcc cctgcgatgc cgcacttgac ctggctaaag
    33661 gtgtcgatgt catggtgcac gaagcgacgc tggatataac catggaagcc aaagccaata
    33721 gtcgcggcca tagctctaca cgccaggctg cgacactagc ccgtgaggct ggagtcggta
    33781 agctaatcat tacccacgtc agctcgcgct atgatgacaa aggttgtcag cacctgttac
    33841 gtgaatgcag gtcaattttc ccggcgactg aactagcgaa tgatttcacc gtgtttaacg
    33901 tttaactctg cttattatta aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct
    33961 acatatatcc acccacacgg gcatattgag cgatttttga tatttattca tctccttacg
    34021 cactatattc agagcaataa gttgcctctt tttatcttca ttcagcacaa cctttgcaac
    34081 taaaaggagc ccatgatggt tacagttgtc agcaattatt gtcaattatc tcaaacccaa
    34141 ctcagtcaga catttgcaga aaaatttact gtgaccgagg aattactgca gtctttaaaa
    34201 aaaacagcgt tatccggaga tgaagaaagt attgagttac tgcataatat tgcgttaggt
    34261 tatgatgaat ttgaaaaaaa agctgaagat attctttacc atattgttag aaacccaaca
    34321 aatgagaccc tatcgattat ccgacttatt aaaaatgcct gtttaaaatt atataatttg
    34381 gcacataccg caaccaactc ccaccttaaa ccaactggtc cggataattc agatgttctc
    34441 ctgttcaaaa agctattctc cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct
    34501 cttatatctg aaaaacagtc gctttcaaag gtgcttttaa atgatgagaa taatgaactg
    34561 agtgatgata caaacttctg ggataaaaat cgtcaattaa ccacagatga aatagcttgc
    34621 tatcttcaga agatcgccgc taatgcaaaa aatactcaag tcaattatcc tactggtctc
    34681 tacgtcccct actccaccag aactcacctg gaagacgctc tcaatgaaaa tattaagagc
    34741 gatccatcat ggccgaatga agtccagtta ttccacataa atactggcgg acactggata
    34801 ttagtttcgc tacagaaaat agtaaataaa aaaaataata aactacaaat aaaatgcgtc
    34861 atattcaact cattgcgtgc actaggccat gataaagaaa attcacttaa gcgtgtc
//
LOCUS       DAZGTR010000038        34597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGTR010000038 DAZGTR010000000
VERSION     DAZGTR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.038"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1248)
                     /gene="amiC"
                     /locus_tag="ACHLCY_002047"
     CDS             complement(<1..1248)
                     /gene="amiC"
                     /locus_tag="ACHLCY_002047"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015963213.1"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP0369305.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARR"
     gene            1480..2811
                     /gene="argA"
                     /locus_tag="ACHLCY_002048"
     CDS             1480..2811
                     /gene="argA"
                     /locus_tag="ACHLCY_002048"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP0369306.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2873..4699)
                     /gene="recD"
                     /locus_tag="ACHLCY_002049"
     CDS             complement(2873..4699)
                     /gene="recD"
                     /locus_tag="ACHLCY_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP0369307.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMVL
                     CGDRLYLNRMWCNEQTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVVSGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHACNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4699..8241)
                     /gene="recB"
                     /locus_tag="ACHLCY_002050"
     CDS             complement(4699..8241)
                     /gene="recB"
                     /locus_tag="ACHLCY_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP0369308.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLCTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARMLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8234..11122)
                     /gene="ptrA"
                     /locus_tag="ACHLCY_002051"
     CDS             complement(8234..11122)
                     /gene="ptrA"
                     /locus_tag="ACHLCY_002051"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP0369309.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEV
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQHLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEDQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11298..14666)
                     /gene="recC"
                     /locus_tag="ACHLCY_002052"
     CDS             complement(11298..14666)
                     /gene="recC"
                     /locus_tag="ACHLCY_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP0369310.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLADDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSVEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPDDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14679..15002)
                     /locus_tag="ACHLCY_002053"
     CDS             complement(14679..15002)
                     /locus_tag="ACHLCY_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP0369311.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(14987..15394)
                     /locus_tag="ACHLCY_002054"
     CDS             complement(14987..15394)
                     /locus_tag="ACHLCY_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP0369312.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(15391..15954)
                     /locus_tag="ACHLCY_002055"
     CDS             complement(15391..15954)
                     /locus_tag="ACHLCY_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0369313.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(15945..16415)
                     /locus_tag="ACHLCY_002056"
     CDS             complement(15945..16415)
                     /locus_tag="ACHLCY_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0369314.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVIVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16600..17394)
                     /gene="thyA"
                     /locus_tag="ACHLCY_002057"
     CDS             complement(16600..17394)
                     /gene="thyA"
                     /locus_tag="ACHLCY_002057"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0369315.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17401..18276)
                     /gene="lgt"
                     /locus_tag="ACHLCY_002058"
     CDS             complement(17401..18276)
                     /gene="lgt"
                     /locus_tag="ACHLCY_002058"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP0369316.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18427..20673)
                     /gene="ptsP"
                     /locus_tag="ACHLCY_002059"
     CDS             complement(18427..20673)
                     /gene="ptsP"
                     /locus_tag="ACHLCY_002059"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0369317.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20686..21216)
                     /gene="rppH"
                     /locus_tag="ACHLCY_002060"
     CDS             complement(20686..21216)
                     /gene="rppH"
                     /locus_tag="ACHLCY_002060"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP0369318.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21569..21715)
                     /gene="ygdT"
                     /locus_tag="ACHLCY_002061"
     CDS             complement(21569..21715)
                     /gene="ygdT"
                     /locus_tag="ACHLCY_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP0369319.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21901..22590
                     /gene="mutH"
                     /locus_tag="ACHLCY_002062"
     CDS             21901..22590
                     /gene="mutH"
                     /locus_tag="ACHLCY_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP0369320.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22659..23372
                     /locus_tag="ACHLCY_002063"
     CDS             22659..23372
                     /locus_tag="ACHLCY_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0369321.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23510..23728
                     /gene="ygdR"
                     /locus_tag="ACHLCY_002064"
     CDS             23510..23728
                     /gene="ygdR"
                     /locus_tag="ACHLCY_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP0369322.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23836..24876
                     /locus_tag="ACHLCY_002065"
     CDS             23836..24876
                     /locus_tag="ACHLCY_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP0369323.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24908..26101)
                     /gene="lplT"
                     /locus_tag="ACHLCY_002066"
     CDS             complement(24908..26101)
                     /gene="lplT"
                     /locus_tag="ACHLCY_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP0369324.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26094..28253)
                     /gene="aas"
                     /locus_tag="ACHLCY_002067"
     CDS             complement(26094..28253)
                     /gene="aas"
                     /locus_tag="ACHLCY_002067"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP0369325.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28839..29870
                     /gene="galR"
                     /locus_tag="ACHLCY_002068"
     CDS             28839..29870
                     /gene="galR"
                     /locus_tag="ACHLCY_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP0369326.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29877..31139)
                     /gene="lysA"
                     /locus_tag="ACHLCY_002069"
     CDS             complement(29877..31139)
                     /gene="lysA"
                     /locus_tag="ACHLCY_002069"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP0369327.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31261..32196
                     /gene="lysR"
                     /locus_tag="ACHLCY_002070"
     CDS             31261..32196
                     /gene="lysR"
                     /locus_tag="ACHLCY_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP0369328.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32183..32875)
                     /gene="ygeA"
                     /locus_tag="ACHLCY_002071"
     CDS             complement(32183..32875)
                     /gene="ygeA"
                     /locus_tag="ACHLCY_002071"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP0369329.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33004..34422)
                     /gene="araE"
                     /locus_tag="ACHLCY_002072"
     CDS             complement(33004..34422)
                     /gene="araE"
                     /locus_tag="ACHLCY_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP0369330.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8457 a         9513 c         8563 g         8064 t
ORIGIN      
        1 ccttctcgcc agcgtcgccc catcggcaaa atacgcttta atccccgcaa gaatagactc
       61 cgcaacttcc tgctgaaagg tcgccgtttt cagtttacgc tcttcctcaa cgttactgat
      121 aaacgccgtt tcgaccagaa tggagggaat atctggtgcc tttagtacgg caaacccggc
      181 ctgttcaacc tgatttttat gcagcttgtt gattttaccg agcttattca gtaccgcttt
      241 accaaactta aggctgtcag caatggtcag cgactgcacc atatcgaaca tggtgtggtc
      301 gacatagcgg tcaccgcttt tgctcacgcc accaatcaag tccgaggcgt tctgggtttg
      361 tgccagatat tttgccgcag tactggttgc acctttggtt gagagcgcaa acacagagga
      421 accgctcggc tgacgactgg taaaggcgtc ggcatggata gagacaaaca ggtcagcacg
      481 ctgtttctgg gcttttgcta cgcgcacttg caacggaatg aagatgtctt cattgcgcgt
      541 catgtacacc ttcatattgc cctctttctc gatcagagag cgcaaacggc gagctatttg
      601 caataccaca tctttttctc gcgttttgta tttccccacc gcaccggagt cttcgccacc
      661 gtggccaggg tcaagcataa tgacaatcgg acgatcgcgc cctgctttac ccggttgtgg
      721 accactttgt gctggcggca cctgcttttc gaggtcgcct ttgttgtaat cctccagcag
      781 cgccagcagc gggtcctgca tatcctgtgc attggccgga tagaggtcca tcaccagacg
      841 ctctttaaac ccggcgaccg gcgcaagggc aaacagctgc ggttttacgt tttgctttaa
      901 ttcaaaaacc atccgtacgg tctgcgggtc aaattgcccg acgcgcgccg acttgatgaa
      961 cgggtcgtct gcacgaattt gtgccgccat ccccttgagt accgagttca ggtttacatc
     1021 ttcgatatcc accaccacgc gttccgggtt actcaacgcg aactgcttat atttcagctg
     1081 acgatttgat tctaccgtca cacgggtgta gctggacgca ggccagacgc gcaccgccac
     1141 gacctggctg accgcagcca gactgacctg acttacgctc aatagccaca tggcacccgc
     1201 gccttgcagt aaacgacgac ggctgattgc agtgttggat cctgacatgc ctctcccgag
     1261 caaaagaaat ctaagctgtg taacaagtaa acgactaatt tgaccggaaa ctttagcgaa
     1321 agacgcataa tctgtcatct aataaacggt aaacattctt tttatattca cggcattact
     1381 gataaaaaag tcgctctcgc ataaaattta cacttgcacc ctgcgaaaaa acagaataaa
     1441 aatacactaa tttcgaataa tcatgcaaag gggtgtgccg tggtaaagga acgtaaaacc
     1501 gagctggtcg agggattccg ccattcggtt ccctatatca atacccaccg gggaaaaacg
     1561 tttgtcatca tgctcggcgg tgaagccatt gagcatgaga atttctccag tatcgttaat
     1621 gatatcgggt tgttgcacag cctcggcatc cgtctggtgg tggtctatgg cgcacgtccg
     1681 cagatcgacg caaatctggc cgcgcatcac cacgaaccgc tgtatcacaa gaatatacgt
     1741 gtgaccgacg ccaaaacact ggaactggtg aagcaggctg cgggaacatt gcaactggac
     1801 atcactgctc gcctgtcgat gagtctcaat aacacgccgc tacagggcgc gcatatcaac
     1861 gtcgtcagtg gcaattttat tattgcccag ccgctgggcg tcgatgacgg cgtggattac
     1921 tgccatagcg ggcgtatccg gcggattgat gaagacgcga tccatcgtca actggacagc
     1981 ggtgcaatag tgctaatggg gccggtcgct gtttcagtca ctggcgagag ctttaatctg
     2041 acctcggaag agattgccac tcaactggcc atcaaactga aagctgaaaa gatgattggt
     2101 ttttgctctt cccaaggcgt cactaatgac gacggtgata ttgtctccga acttttccct
     2161 aacgaagcgc aagcacgggt agaagcccag gaagagaaag gcgattacaa ctccggtacg
     2221 gtgcgctttt tgcgtggcgc agtgaaagcc tgccgcagcg gcgtgcgtcg ctgtcattta
     2281 atcagttatc aggaagatgg cgcgctgttg caagagttgt tctcacgcga cggtatcggt
     2341 acgcagattg tgatggaaag cgccgagcag attcgtcgcg caacaatcaa cgatattggc
     2401 ggtattctgg agttgattcg cccgctggag cagcaaggta ttctggtgcg ccgttctcgc
     2461 gagcagctgg agatggaaat cgacaaattc accattattc agcgcgataa cacgactatt
     2521 gcctgcgccg cgctctatcc gttcccggaa gagaagattg gggaaatggc ctgtgtggca
     2581 gttcacccgg attaccgcag ttcatcacgg ggcgaggttc tgctggaacg cattgccgct
     2641 caggcgaagc agagcggctt aagcaaatta tttgtgctga ccacgcgcag tattcactgg
     2701 ttccaggaac gtggatttac cccagtggat attgatttac tgcccgagag caaaaagcag
     2761 ttgtacaact accagcgtaa atccaaagtg ttgatggcgg atttggggta agcgatgaaa
     2821 atcgccggat gcgacatgcg taacactcgt gcgtcgcatc aggcaattac gtttattccc
     2881 gcgaactaaa caatgccgcc agaccactgc gccgctcagt gcgcgtggcg attgccgcac
     2941 ttaatatgcg ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc actgcggtgt
     3001 aaaccagctc tcgcgttact accggcgtgc gttggctcgg taaaatcaac gccgcatggt
     3061 cgaactccga tccctgcgat ttatgtaccg tcatcgccca cgtcgtttcg tgctctggca
     3121 ggcggctcgg ttgcacagac ttaatattgc cgtccggcat cgcaaaccaa acgcgcgtcc
     3181 cctgcccgcg atccagcgca ataccgatat cgccattaaa caacccaagc gcgctgtcat
     3241 tacgggcaat catcaccggt cgaccttcgt accaacgaga gtgcggatga cgatgaattt
     3301 tgcgcttctg ttgcataaac tgctcaattc gctcattcag tccagccacg ccaaacggcc
     3361 cttcccgcag ggcgcacaaa agttggtact cattgaacgc ctgaatgatt aaatccggct
     3421 cggcacgcgc ttgcagcaga tccagataac gtccataacc cgcaagagct tcctcaagca
     3481 tcgcaatata atcttctccg ctctgtaaaa gtcgtttttc gatatcagta aaatcctgct
     3541 gaaaaacggt tttcactgcc gttttatcac cacggttgat cgccgcagct aactgaccaa
     3601 tgccagaatc gctgccgaaa cgatagcttt tttgcagcag gcagagactg tcgcgcaaag
     3661 atgccgcttc tgtgccggtt cctgccggaa cgtgagtccc cgtcaggcgg cttagctgcc
     3721 tggcacgctc ggcggtaaag cccgcgttgg cataagcgca gatatcgccc agcacagccc
     3781 cagcctcaac cgaggccagt tgatcacgat cgccgagaaa gatcactcgc gcatgatctg
     3841 gcaaggcgtc gatcagtctc gacatcatag gcagatcgat cattgacgct tcatctacca
     3901 ccagcacatc aagatgcagc gggttacagg catgatgacg taaacgctgg ctacccggct
     3961 gcgcgcccag caatcggtgc aaagtgctgg catcttccgg aatgcgtttc ttttgttcat
     4021 cggtcagcgg taactgtcgc aaagccttgc cgagagattc ggttaagcgc gcggcagctt
     4081 tacccgttgg tgcagccaga cggatacggc agcgttcgcc gtcggccatt tgaattaacg
     4141 ctgccagcaa cttcgccacg gtggtcgttt taccggtgcc tgggccgccg gaaaccaccg
     4201 agatccgccg cgtcagcgcc actgccgctg caactttttg ccagttaatt tcatcgctta
     4261 ctggaaaaag tttgtccagg gtttgcgcca gtagagcttc atcaacctca atggcatgat
     4321 tcacctcatt gaagaagcgc gctaccgtct gctcgttaca ccacatgcga ttcaaataaa
     4381 gacgatcgcc acagaggacc atcggcgtgg gttcatctcc cctgctgacc gcttgagaag
     4441 ccagcaagca ttcttcccaa ttttgtagct caccgatttc actgacacag gtcgccaaca
     4501 gcggatgcga cgcctcgtta ttttccagtc gtgaaagcgg caaacagacg tgtccctctc
     4561 cggcatcatg acttaacagt gccgccgcga gggtgacggc aggatgttca tctcccgcca
     4621 cggtcagggc aaattgcaca tccagcgggc gtagctgttt gtgctccaca gcttccagta
     4681 attgcttttg caatttcatt acgcctcctc cagggtcata ccggcaaaca tctcatccat
     4741 cagggcaatc aacccggcgt tgggtcgggt tgtgtaaatc ccttgttgcg gatgttcttt
     4801 atcaacgcca cgcaggaaca gatagataac gccgccaaag tgacgatcat agtcgtaatc
     4861 agcaatgcga tggcgcagat aacgatgcag cgccagggta taaagctgat attgcagatc
     4921 atagcggtgt gcctgcatcg ctgctgccat agcctgttgg gtgtaagccg aactgtcttc
     4981 acccagccag ttggatttat agtcgagcag gtaataacgc ccttcgtggc ggaataccag
     5041 gtcgataaag ccttttaaca tgcctcgtac ctgcatgaac tccagcggcg ggcagccagc
     5101 ggatagcggg tcaaactggc gaattagtgc atcaagctgg ctggcgatga gcggttcgct
     5161 aatcggcagg taaaactcca tctccacctg tttatcgcgg tcggaaagct gactcaggct
     5221 aacacccgtt tcattgagag gtgcctggag gacagccgtg atccactcgg tcaacaccgg
     5281 ttcccactgc gattcaaagc cgccgagttc cagtttttcc tgtacccagt tcgggtcaac
     5341 cggctgggta aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc
     5401 gcgcgggaac tgatgcggtg ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc
     5461 atcaacatcc agccgtggca tcagatcctg agcgataccg tgaccacgct gctgcaagcc
     5521 ggagtagctg gtgacacgcc agttatcgcc gggcaatcgt tgtagcatcc tcgcattcag
     5581 ttctgcagtt aaagcatcat taacctgcca gggctggtta tcaccagttt gtgccgtttg
     5641 ccaggcaata tcatcatcgc ataacgcttc aatacaggtg caaagccctg ccgcatcttg
     5701 cggttcccct ttttgcagca aacgcccgag cgcactttgg tggacgtcgg tgtcaccttt
     5761 tttatcgcca cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg
     5821 cgtcagcgcc acgtaaagca aacgcagatc ttccgccaga cgttcgacct ccgcaagctc
     5881 gacgctttct ggcgcagcgt taagatccag aacagcctca aacgagtggc gatcgtgata
     5941 aaacgcctga tcctggacgc ggaaattggt aataaatggc aaccagacca gcggatattc
     6001 cagccctttc gatttatgga tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg
     6061 catttgttgg ctggaggcat tactgtctgg ctcgaggata tgttgcgata accagcgtac
     6121 cagcgcatgt tcactttcca gctgcgttcc ggcttcttgt agcagttcgc tgatatgcaa
     6181 gatatcggta agacgccgct caccgcctgc cgttgccagc aagttttctg caatgttacg
     6241 cgccgacatc agcgcccgca gcatcggcat aacgccacgt ttgcgccaga tttgccgata
     6301 accatcgaac tcttcaacta ccgcatccca cgcatgttcg tcattattca gcgtttcgat
     6361 atccagcgcg ttcagcccca tcattgacgt tgccagcgca ctgcgcaggg tgttctcacg
     6421 ttcgggcgtc atcaccgcct gcaacaacca aagcatttcc tgcgcttcca gagtttcaaa
     6481 aacactgtcg cggttcgaaa ggtaaacgga agggatttcc agcaacgtta aggcatcgcg
     6541 cacctgggcg gcctcctggc ggctgcgcac cagcacactg atgtccgaag cacgcaccgg
     6601 acgcgcgtcg tcgccgttca tcagcaacgc ttcgccccgc tgtccggctt gtagccagtc
     6661 gcggatttgc gcagcacata cctgcgccat ggtactttga taatcgccaa cgccgcagct
     6721 ttcgccttcc atcagccaca ttttcatcgc aggctgcgtt tcacctttaa acacaaaacg
     6781 taacgcctga tttttcccgg ctgatttcac tggaataaac ggtatttcgc gaaacatgaa
     6841 cgcgtcatca gtctggctga aaagcttgtt cacactgttc accattccag gtgcggagcg
     6901 ccagttggtg tctaaagtgt agtgggcgtg aacttcacta cgcgccttca tataagtgaa
     6961 gatatccgca ccccggaatg catatatggc ctgcttcggg tcgccaatca gcaacaatgc
     7021 ggtttccggc tgatggtgcc agatacggcg aaaaattcgg tactgctggg ggtcggtatc
     7081 ctgaaattca tcgatcattg ccaccgggaa tcgcgtacgg atcgccgctg ccaacacctc
     7141 accgctttcg ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa aacccaattc
     7201 gccacggcgg cgtttttcac gcgctactgt ttcgcggatc tcagccaatg cgcgggtgat
     7261 caccagatcg cggatcgaca atggttctgc aagcagttga tcgatcgcct caaacagtgg
     7321 atgtcgcggg gtttcccccc cggccttcgt gcgatcttct aagaaacgct gggagaattt
     7381 ttccagcgac tccggcaact gataactgtt tgtctcttct tctgcccagg cgctgatctt
     7441 ttcgatccat ttagcctgat tgctacggtt aaacttgcgt cgatcaatac cagaagattc
     7501 gatcagcgca tcaagttcac ccactgcgtc gcgccactgc tgttttaccg tatcaatacg
     7561 cgccacaatt tgcgcgtggc gggaagccag cgtttcatca tcgggcggcg gtgctttgat
     7621 aaccggtgct tcgccttgca gataacgatt aatatcgcgc agcaacgcct gcggcccttt
     7681 ccaggtttca aagaccacca gggcaatttc acgcggcagc gggtagcagt ggcgacgcca
     7741 gaaatcggcg caggcctggt agcgtagcag agactcatct tcaatcagct gctgctcaaa
     7801 cagcataccg gattcaaagg cattcaggtt gagcatgcgc tggcaaaaac cgtgaatagt
     7861 aaagactgcc gcttcatcca tctgccgttc ggctaacaac aaccactgcg cggcttgcgc
     7921 tttatcgtcg atctcttcca gcaggcgttc gtacagtgga ttgtcggtgg tttcacgcag
     7981 acaggcgatg cgcaactcgt ggatattgct acggatacga ccacgcaatt ctgccgtggc
     8041 agcctcggta aaggtgacca ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc
     8101 ggaaccgcct agtccaagta acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc
     8161 tgtgccggca gaggcttcaa tcaggcgctc gccctgtaag ggcaagcgca aaggatctag
     8221 tgtctcggcg acatcactca ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc
     8281 tgacgttctc ccacactttc cagccttcag gatgcacata ttcggctttc ccgttctggc
     8341 tgccggaaat ctgcgacaga atagccatgc cttgcggctc gaccaccgcc tgatggaaga
     8401 aatcagcaag tttttgcggc gtcagcagtt ttatctgggc cacgatttta tcacgcgaat
     8461 cgaagcgcat attgccgcga tcgaaatctt tacttaactt cgatgcttct tcgccgagcg
     8521 tttgcggtgc ctgcagcatc tgggtaatta ccgcctgctg gatttgcgca aactcttctg
     8581 gcttcatcgc ccgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca
     8641 agaaggaagg ctgtttatca ttgctttgca aaaggaagcc catgccccac tgacgcccca
     8701 cgctcattgg aaatgcaaac acggcatagc ccaattgttc ttcggtacgc aactgattgt
     8761 agaaccacgg ctgtacgatc tgccccaaca gagagctata ggctgagctg gtgtattcat
     8821 cgtagccagt cggtacaaat accgctgcca gtgcggagtc ggtgctgtta ccagcttttt
     8881 caaagatgac ggattgtttt ttatcgacca cgacatcttt gttacgacac cactctgaac
     8941 catcagcgcc caactgtttt tgcacatcgc gtgccagcgt tgttgcctgg tcctcggtca
     9001 tgttgccgat aaccataaac tctggtcgag cccctgattt taaggcgtcg cgataggcca
     9061 gcacctcttt caacgtaatg gagggcaaaa ttttgcgccg ttcatctcgc gagaagtacg
     9121 gcacttgcga gagcatctgc gcgggcataa tcgcctgctc gaacgctttg cccttttctg
     9181 cggaatccat catctggtta taccaggact tcgcctgctc aagctgatct tccgtggcgg
     9241 tatagctaaa gtagccttcc agcaatgcct ggaacagctg cggcagatgc tgggtgtaac
     9301 cattagcatt aaccataagg ccgttgttag cgttggtgga aaaacttatg ccaccaaccg
     9361 acgcctggtt gcttaactga tcaagcgcca gccctgcgag ataatcattg agcgcaaaca
     9421 tcacctgatt gcgggcgctg tccatggctt tcggattacg caaaatcagg ctgacatcgg
     9481 cttttggctc gctggcaaaa taacggcttg gcgcatacac cacgcgcaga ttcgactcat
     9541 caacaatcag ctctggatgg tcatatttct tgtctgactt aatcagcgag aaatcgtcag
     9601 gaatataagg gttaagctct ggcaaagaga gcgcaatgtc ggcggctttt ttctgccagt
     9661 cggcgaaagt ttgtgcgctg attttatcga cctgatacgg cgcatcgaca aagtaagccg
     9721 ttttgttgtg cggctctttc gggctgatat accagatacg cgcattctgc ggcgtcatca
     9781 tcgccagacg ttcctttact gctttagcat cgtaccgatc ggcaatattg actgcatcca
     9841 gcgtatgctc aacaggaacg cgaatcatgg tatctgccag ccattcgacg taatccatat
     9901 cacgggtgat tgacggataa cggaagtcga tatccagcac attcgccact tcatcgaagt
     9961 attgtttatc gatccctttt tcacgtaaca aattgaggta gctaaaaata gccgccacaa
    10021 cctgatcgcg attcgccaga cctttatcgg ttaaagacgc agagatcgct aatacgccgc
    10081 tgttgccgtt gacgatagga tcggagttgg cgctaatgcc ctcaactaat ccctgctttt
    10141 gcagccagtc ggaaagtgta cctgggctgc gattaccaat cagataggta atcaattcat
    10201 cggttttgct acggaatttc gccgagttgt tatcaatacg aaactctacg cgcaacactt
    10261 tacgcggcag cgccgggacg taatgaatga taatgccctt ttgcgcgtcg gtgactaccg
    10321 gcacggtgat ttccggtttt ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg
    10381 ccatttttgc caactccggc aacggtttat tgctgtaaat aaccgccttc atcagattgg
    10441 cggaatagta cttctcgtgg aaatctttca gcgcctgctg caccggatta ccaggtttgt
    10501 cgcttaaagt ttcgaggtta ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg
    10561 tttctgcgct gacctgtgcc atgcgcatcc cgtcacgcgt acgcgccatg gttaattcag
    10621 cgttcactgc attacgctca cgttcggcgt atttcttgtc gagcaagggt tctgcaatag
    10681 catcagccag gcggtctacc gcaccaggca aggcgtcgtt ctcaacttcc agatagaaag
    10741 ccgtgcgata cggcgcagtg ctggcattgt gactaccgcc gtgcattttg agatattcgg
    10801 ccagactgtc agcctgcggg tactttttcg accccatcag actcatatgt tcaaggtaat
    10861 gtgccagccc ctggtacgcc tcgggatctt ccagcgaccc aacgggcacc accagcgccg
    10921 agagcgattt aactgcctgc ggatcagaaa ccagcaagac caccataccg ttatccagac
    10981 gtatagcctg atactggcgg ttatctttat cacttttacg gatggtttcc tgaatcggct
    11041 gccatcccgt ttctgcctga cttaagggtg cccaaagggc aactaacaac aataatgctt
    11101 tgaaccaggt gctgcggggc attcacggac ctcataatca acttaatttt ctgtccagat
    11161 tcaacacgtt aacgcaaatc atctgccaga atttaatctt gtgctgcacg agtcagccta
    11221 tgtttatata accatcagtc cgtgactggt gcgcatcata aagtaagcgg atagattgcg
    11281 caatttttat acaacactca tgactgatta aagcgaaaca gcggtaacag gaaacgttgc
    11341 gactgttcaa cgatggcctc cattgtctct ggtgttaatt gccgccagag cctttgatac
    11401 cagatatcat caccttcgcc acgcaccatc atgttgcctt cgtaagcctg aaggaatttc
    11461 gtacgggctt tttgcaacgt ggaatcgtca tccagcatgg catcgttttg cgcgtcataa
    11521 caggttttta gccacgcgcc gccactttca ggtaacacca gcaatggcgc ggacatccct
    11581 tcacgatacc cctcaatcag ttgtgagagg taatgcaaag cctgttcggc tgcaagcggc
    11641 ggaaaacgcc actcgccgtc tttgcgtaga aaaaggcgac tttcaccatt accaccgctg
    11701 gcacagtaga caaggtgttc cagccaaagt tgcattccct gcgccacact taataaagag
    11761 ggacgccagc gcaacaggcc atccggctgc acctgcggca accagccagt tatctgcaca
    11821 ccgttgcagg cgagatcaat ttccatactc tgccccggct ggcgacaggc aatgactctg
    11881 tcggcaagct gctgcatctc ctggcactgt gtttcccaga aaatttcacc aaaagcgcca
    11941 tacggtaaat cccctgccgc ccggaagcgg cggaacaagc gttcggcatc atcctgctca
    12001 accagtgcat tcaataactg ctgattgatt tgataacggc taagcccttc cagaataaat
    12061 ggctcggtgt ctgggatttc gctgtcttca gtacggaagt tcacctgcaa acgcatctgg
    12121 aagaacgccc gcaccggatg tgcccagaat cgttgtagcg tttccagcgg cacggtttcc
    12181 ggtaaggtaa acggcagcgg ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc
    12241 gcaggtagcc attcacgagc atagctttgt cgttcgcctg gctggtagtt ttgcggatca
    12301 aacggcatcc gggtatggag gcaagtaaga tgcgctttta cccttgcctc gctttcatca
    12361 cagttgagcg cttcatcgtc cggtagataa tgactttgcc cgatgtagtc gatcagttcc
    12421 tgcaccagta ccgacgggaa acgttcactg ttatcctgaa tggaacgccc gatatagctg
    12481 atatagagtt tttgctgcgc ggaaattaac gcttccagga acaggtagcg gtcgtcatcg
    12541 cgacggctac ggtcgccacg cttcggtttc tggctcatca ggtcaaagcc caatggcgca
    12601 agctgacgtg gataaacgcc gtcgttcatt cccagcaggc aaaccacttt gaacggaatt
    12661 gaacgcattg gcatcagagt acaaatgtta accggtccgg cgagaaaacg ctggctgata
    12721 cgttcttgat ccagacgctg cgccagttca tcacgcaata gtgacagcgg caccgcgtcg
    12781 ccatactgcg cacctaaacc ttcggcgata atcgcctgcc attgttgttc gatcagcgtc
    12841 atcgccgctt cggtttccgc atccggcagg aagaaggcgt tgagcatatc gcgacaaacc
    12901 ggcaaccact cttctagcgg acgctcctgt gccagcccgc gacgccagat gtttagctgc
    12961 attagcagtg aagccagatg ccccaccagt tctgcaatta agccgctcga ttcatcataa
    13021 ggtagaaccg attgccactc gccctgcgcg ctctccatcg cgtagcccag caacatacgc
    13081 gtcaggccaa atcgccaggt gtgttgtccg gtggcgggga gttccagctc gcgaacgttg
    13141 tcgtcatcta tcccccaacg aatgccggat tcgttgaccc actggcgtaa ataacgcagc
    13201 ccttcttcgg tgatgtcaaa ccgcgccgcc agcaccggca catccagtaa tgccagcacg
    13261 tcttccgaca caaagcggct gtctggcaac gataacaggc tgataaacgc ttccagtacc
    13321 ggatgcgact gccgcgcccg acggtcggaa atggcgtaag gcaggtaacg atccgcaggt
    13381 gcgctaccaa acacagcctg aataaacgga ctgtagctgt cgatatcagc caccatcacg
    13441 atgatgtcgc gcggagtaag tgtcgggtct tcttccagca tcgccagcag gcgatcgtgc
    13501 aaaacttcaa cttcacgctg cgggctatgg caaacgtgga aggtgatact gctatccagt
    13561 ggatcaagcg ggcgtttgtt atcgctacgg gaaaactctt cgatgttcac accagcaacg
    13621 gcacggtttt ccagttccag aatgtcagac tgaatgttat gcagcaggtt atctggcgtg
    13681 acatcgacaa aggcgtccag ctcctggctg ctctccaggt cagaaaggag ataaatgtag
    13741 tcgcgcccaa gcttacccca tgaagccagc agcgggttgc cgacatcctg ttcaccatcg
    13801 ctgttaaaga gctgcccggc attttcgcta tcacgaaata gcggcaattc gcgatcttca
    13861 acactgtgtc gacgctgacg ggtcagcagt ttcgccagat aagcaggatc tttaatatcg
    13921 ccccagtaat aacggcaggg gttggtaaac aggagatgga tttcaatatg tttacccagc
    13981 gcctggagcg cctggagata aacaggcggt aacgcggaaa taccgcatat aaagacgcgc
    14041 gaaggtaacc ccggcgggca ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag
    14101 agattggcgc ggtgccaacg cggttgtccg agttcatggg tatattccac cagcgccttc
    14161 cacaacggcg cttgccaggc ctgtgcttct cccagccctt caaccaaatg tcctgtttcc
    14221 cactgtgcca gccactccgg gcgatagacc agatactggt caaacaggtc cgccgctttt
    14281 gaggaaagct ggaacagttt tcgcttgtcg ctatcgtcag ccagataatg ccgcaacagg
    14341 gtaaagtctt cgcgatccag caattgcggc agcagagtca tcagtttcca gctcatgctc
    14401 tgtttgttaa aggcgctctc tttggggatc tccggtaaca cccggacgaa catatcccag
    14461 ataaagctcg ctggcagcgg aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg
    14521 gtcatttgca gccactgcgc cataccggta ctttgcacca gaatcatctc tggttcgaaa
    14581 ggatcgtcca gccgttcgcg ttcgacaata aactccatca acgcttccag cacgtccaga
    14641 cgattggaat ggtagaccct taacatagcg gctcctgact actgacgatt cgggcaatgc
    14701 aggcgggtca tctcgccttc tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca
    14761 catcccgcct gcgatgtctg cattcggttg acctgccagt tggcaggtgg cgaaatcgcg
    14821 cgcagttgcg tttgctgcca accatgccgc cagagctgtt ggtactggtt tctgctggcg
    14881 agactgttca ttaatgttcg ctggataccc gataacgcag tgacaatcat caccatcagc
    14941 accatcgcaa ccattacctc cggcaggcta aagccttgtt gattcttcag ggaagctgac
    15001 ataacgccct ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa aaaacaatgt
    15061 ttccatcaat gacttcgcct gttcgccaca acgaaacgcc ttcataaccg gcaaccaata
    15121 aggcttcatt atctgccagt aaacgcaaac aaacccgggc atcggttcca gcgtactgcg
    15181 agcactgaac tgcgggctgc gtctgccagg agtgcatttt cccccacgcc agcgccgact
    15241 gaacgatggc ctggcggcgc aatgactgac tttccatgct cacgcgagag gcaaaactgc
    15301 gatcctgctg actcattcct tgcaatagca agctacccaa aaccagcagc atcaggacca
    15361 gagccagtga cgaaacacct ttttcgcggt tcacaggttg aatcctgtca cgctatagct
    15421 ggcatccacc acggtttgcg gttcagactt actggcagca cgcatattaa ccgtcaacac
    15481 cggcgagaag ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc
    15541 cggattagtg actttatccc agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc
    15601 cagcacatgc tccttcagac gaaatccaat ctggtcagac tcttttaccg gttcgcgatc
    15661 ccagataccg ttactgttcg catcccactg cacaatgata cagtcaccct gtccgacaat
    15721 ttccagccct tcgccggtac agctgccatg acaataaccc gccctctgga gatgcttcgc
    15781 gacggtaaat acccgcagcc agatttcatc ttccagcgcc agcttacggg tgctcgttaa
    15841 actttcacgc tgtaacgcag gcagaaagcg tgcagccccc agcaacaata cgctactgat
    15901 cgccatagca atcaacactt ccagcagaga aaaaccttgc tcttttacag gcatccttct
    15961 gtttctcctt gctgacaaag ccggagtctt ccccacggcg aaaccaccag ccaccactcg
    16021 cccgttgagt ttttgaagcg aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa
    16081 gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc agcgtggcac aatgaccaat
    16141 ggtgaactgc catgacaggt attggcccca gcagcggaac tcacaaggca ccataacgtc
    16201 ccctccctga taacgctgat actgtggtcg cggttatgcc agttggcatc ttcacgtaaa
    16261 tagagcaaat agtcccgcgc ctggctggcg gtttgccata gccgttgcga ctgctgccag
    16321 tattgccagc catagagtcc acttgcgctt agcatgacca aaatcagcat cgcgaccagc
    16381 gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa
    16441 aaaagacgag ggccagtttc tatttcttcg gcgcatcttc cggactattt acgccgttgc
    16501 aggacgttgc aaaatttcgg gaaggcgtct cgaagaattt aacggagggt aaaaaaaacc
    16561 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    16621 aatgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    16681 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    16741 cagatgagtt tgatccatat gattgctgta caggtgcgtg tcgccccccg tccagacaaa
    16801 atcgcccact tccagatcac actgctgcgc catcatatgc accagtaacg cgtagctggc
    16861 aatgttaaac ggcaggccga ggaaaacgtc acaggagcgc tgataaagct ggcaagagag
    16921 tttgccgtct gccacataga actggaagaa cgcatggcac ggtgccagcg ccattttatc
    16981 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    17041 ctggttcagt accgtagtga tctggtcaat atgacgacca tccggcgttg gccaggcgcg
    17101 ccactgttta ccatacactg gcccgaggtc gccgttttca tcggcccatt cgtcccagat
    17161 ggtgacattg ttttcgtgta gataagcaat gttagtgtcg ccctgcagaa accacagcag
    17221 ttcatggatg atggaacgca ggtggcaacg tttagttgtc accagcggga atccatcctg
    17281 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    17341 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    17401 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    17461 gacaatcatc gggatggaaa gaatttgtcc catgctgatg tactgcaccc aggcaccagt
    17521 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtaaccaat
    17581 caggaacaaa cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    17641 aataaacagc actacacctt ccagcaacag ctcgtaaagc tgtgatgggt ggcgcggcag
    17701 cacaccgtaa gtatcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    17761 ttctgtacgg gagccaggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    17821 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    17881 tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat
    17941 caccacgata acgccaatca ggccgccgtg gaaggacatg ccgccgtccc agacacggaa
    18001 cagatacagc ggatcggcca taaactgcgg gaagttgtag aacagaacat aaccaatacg
    18061 tccaccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    18121 ccagccactg cccggacgat tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc
    18181 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    18241 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    18301 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    18361 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    18421 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    18481 aacctgatgg cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    18541 tgcttcggca taatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    18601 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    18661 catttcaccg cacaaacgga gatcgattcc atgtatttcc gcttcccggg cgatcatcgc
    18721 cagagctcgt aacattgcag gatgaagact gtcataaatg ttcgccaccc gggtattgtt
    18781 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    18841 ccgctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    18901 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    18961 caggcggcgt gcttcatcaa cttcatcgag gcttgtgacc atcggcaaca gaatattcag
    19021 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    19081 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    19141 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    19201 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    19261 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacggctgcc
    19321 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    19381 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    19441 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    19501 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    19561 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    19621 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    19681 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    19741 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcgccag cccgctcttt
    19801 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    19861 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    19921 gtccgaaagt aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc
    19981 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    20041 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    20101 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    20161 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactggg aaagaatagc
    20221 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    20281 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    20341 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    20401 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    20461 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    20521 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    20581 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    20641 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    20701 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    20761 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    20821 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    20881 attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat
    20941 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    21001 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    21061 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    21121 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    21181 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    21241 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    21301 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    21361 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    21421 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    21481 ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccagctca
    21541 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg
    21601 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    21661 atggaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    21721 aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc
    21781 gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa
    21841 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    21901 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    21961 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgccgg gctggttacg
    22021 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    22081 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    22141 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta
    22201 acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta
    22261 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca
    22321 ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa
    22381 ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat
    22441 ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    22501 ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca
    22561 ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    22621 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    22681 atcctaacgc ctggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg
    22741 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    22801 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatcg
    22861 cctgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    22921 gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    22981 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    23041 ctatcgtgca gattatgctg ctggatatta tctttagcct cgactcggtg attacggctg
    23101 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    23161 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    23221 tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    23281 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    23341 acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    23401 gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    23461 ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg
    23521 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    23581 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    23641 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    23701 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    23761 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    23821 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    23881 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    23941 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    24001 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    24061 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    24121 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    24181 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag
    24241 cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt
    24301 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    24361 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    24421 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    24481 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    24541 ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt
    24601 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    24661 gcgtatgttg atatcgccag acgtcatggc ctggaccctg ctcagatggc gctcgcgttt
    24721 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg
    24781 aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa
    24841 gcggtgcatc aggtttatac ttatccggca ccataagatt aacgccggtt aaaaccggcg
    24901 ttaaatatta atgacggcgc tgccagatcc acagcgccgt tattgccagc gcaaacagcg
    24961 caccgaagcc aatgccaatg ggcacgaccg ggatgcctat cattaccgcc agcgagtaaa
    25021 tgcccagcat caacaacatg gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc
    25081 ccgccccgac gcttttttta ccccgctcct gtagcaacgc attgagcgga acgacaaaaa
    25141 agccccccat cacgccaatc agcatcaaca aggcataggc tggcagcagc tcgtgttgca
    25201 gggaaaaaat cagtaccacc acgccaatca aaatcccggc tggcatacag cgtgacacgg
    25261 tttccagcgt aactaacttc gctgccgcac ctgcgccaac cacgatacca atcgctacca
    25321 tcgcgttgag ataggtgggc gtagcgttat cggtaatgcc cagcgccacc ggtacccaca
    25381 gcaccaacag gaaacgcagc gtgacacccg ctccccagaa taaactggtg cccaccagcg
    25441 aaaaacgcgt ttcaccattg cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca
    25501 tgttgatgag attccaggac tgccccggac gcgccgccgc cagtttggga atgtagatat
    25561 tggcaacgac cgcaccaccg taggccagtg cgcatgcggc cagggcgacg aggacatgcc
    25621 agtcagccag cacaccaccg gctacggaac cgagcaaaat cgccgctatg gtagaagctt
    25681 ccattaaacc gttagctttc actaacttac tacccgtggt taattcgccg agaataccgt
    25741 atttcgccgg tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat
    25801 tgataccaaa gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca
    25861 cccggccttt ggcgaagcta tccgccacct gcccgacaaa cggcgcaaaa agaatgtaag
    25921 cacctacaaa caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca
    25981 gtaacgccag agtggcaaac agtagggcat tatcgccaaa cgcagagaga aactgcgcca
    26041 cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca
    26101 tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg
    26161 ccaagtaatg gcatctgttt cagatagcga atatcgcgcg gtacagcaag ctccggcacg
    26221 ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa
    26281 agcaccagtg cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct
    26341 ggcgaaacac caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt
    26401 ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca
    26461 atatcgccag tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc
    26521 acttccagta cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc
    26581 agttgcaggc gtccgccctc ttcgatacca ggaaccgaca acaggcgcgc atccattcct
    26641 ggtagaatac gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca
    26701 ggcgcgcact cggttacacc atagccttca aggatgcgca ggccaaattt atcctgccaa
    26761 agctgtttgg tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag
    26821 aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt accgaacaac
    26881 acggtgcaac tgcggtcata caccaactcc ggcacaatgc ggtaatgcag cgggcttgga
    26941 taaaggaaca cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt cagcccaaag
    27001 gagtgaaaca gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt
    27061 ttaatctgct cgacgttcgc cagaatgctt ttatggctat ggacgacgcc ttttggatgg
    27121 ccttcagaac cggaggtaaa gaggatcagc gcctcttctt ccggctgctg ttttacctgc
    27181 gccaggcgcg gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct
    27241 gcttttaaat cttccagata gacccagcgc acctgagtaa gttgctccgg cagatgccag
    27301 agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc agccgccgta
    27361 atagcactgg tcagcccttt taccccagca gtgtagttca tcattgcggg aatgcggcga
    27421 cgcgcgatgg ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg
    27481 atgcgttcgc cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta
    27541 agcaatttgc gataggagtc tggggtaaag ttgacgtctt cgacacattt cttcccggct
    27601 ccgaagcggt acattgcact cagtaaagat tcgtacagag tttcacgcgg gcgcaccgcc
    27661 atacgcgctt ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg
    27721 gcacgcggcg catccggcat cgccacctgc gttggtggca aaatatgcag agtaatttgc
    27781 gggaacaagc gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct
    27841 tcaatacgca caggaataac cgttgcacca gacttcgccg cgacaaaacc cgcgccatcg
    27901 tagattttca tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat caccactggt
    27961 cggccctgtt ccaccagacg taccagatgt ttaatagcca taggttgcgt cgggtcgaga
    28021 ggaacaaagt cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg
    28081 taaacggcaa acactggacg cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa
    28141 gagacgtgat taggcgtaat tagaacgcgc tcgcccttca gtgcctgggt gtcacccgta
    28201 acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact
    28261 ccctttgctc tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga
    28321 gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac
    28381 agggtacgaa gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg
    28441 gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga
    28501 ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat
    28561 ctgcgcaggc tggtgtaatt catgtgctca acccgaagtt gacttcacct atcaatacct
    28621 ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg
    28681 tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg
    28741 gctcacattc ccacgatgaa aacacgcccc cccttgaacc aacgggcgtt ttccgtaaca
    28801 ctgaaagaat gtaagcgttt acctactaag gtattttcat ggcgaccata aaggatgtag
    28861 cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacctaaag
    28921 ccagtgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga
    28981 acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg atcgttggtg
    29041 atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca
    29101 ccggtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcta
    29161 ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccgg
    29221 atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc
    29281 tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc tggctggcaa
    29341 cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta
    29401 tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattg
    29461 cggccaatga ccggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa
    29521 tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa
    29581 tggcggcggg tgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt
    29641 cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg
    29701 tgcgttaccc aatcgtgacg atggcgaccc aggctgccga actggctttg gcgctggcgg
    29761 ataatcgccc tctcccggaa atcactaatg tctttagtcc gacgctggta cgtcgtcatt
    29821 cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa
    29881 agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga
    29941 ccattatcaa acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat
    30001 gcgccatatg cccctgtatc atgcagtacc agataatcac ccgccttcac ttccggcaag
    30061 gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataat
    30121 ggcccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc atcagctgcc
    30181 agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg
    30241 gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt
    30301 aacacgccag actgcgctac cagaaagcga cccggttcaa tttccagttt cacagggtgg
    30361 cccaaatggc gagcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta
    30421 tcaaccgcct cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc
    30481 tgtaaatcct gaccgaattc gatgacctga cgtaccatag caccacacac ctgttccaga
    30541 tgggcataat caacgccaga accaatgtgc atgtgaatgc cgaccagctg caaatgatga
    30601 cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt
    30661 tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccgggtt aacgcgcagc
    30721 cataccctat gccctggcga aacctggccc agttggtcga gcatatcaac agaacccgca
    30781 ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc
    30841 gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct
    30901 atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa tcgcaaaata
    30961 tgaatattgg aacaggcttt ctgtgcaaag cgcaccacat caaactgttt cagcgctgca
    31021 atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc
    31081 aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata
    31141 acaaactcca gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata
    31201 tcgatttatg tcgagtctat gcaaaaatga tatggattac cggattgcga gagagcgcta
    31261 atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc
    31321 ctgactgagg cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg
    31381 cgctttgaga aggtgatcgg gctaaaattg tttgagcgcg tacgtggacg attacatcct
    31441 accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc
    31501 attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc
    31561 ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat
    31621 cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg
    31681 gcccagcgtc atgatttagg actcactgag acactccata cgcctgcggg aacagaacgt
    31741 accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta
    31801 aaaaaggtat taacgccgga tgattttcag ggtgagaact acatcagcct ttcccgtact
    31861 gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg
    31921 atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtgtt
    31981 tcggtggtta acccgctcac cgcactggat tatgcggcaa gcggtttagt ggtacggcgg
    32041 ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca
    32101 gcgctggttc aggcgtttag tgagcattta caagcggggt taccgaaact ggtcacttct
    32161 cttgacgcga ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga
    32221 tcgccgcagt atcaaacaca ggcaaaacac tgcgctcttc tggaaccaat aaaccaattt
    32281 ctgtgcagcc aaaaatgaca ccctgcgccc cctgttctgc aaggcgagca atcacttgcg
    32341 cataataagc gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct
    32401 gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg
    32461 tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac
    32521 gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca
    32581 gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa
    32641 taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt
    32701 tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca
    32761 gcacttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat
    32821 agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc
    32881 tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg
    32941 ccaggtgccc gatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg
    33001 aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc
    33061 cagcgtgaca tttttggttt ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt
    33121 cagcgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa
    33181 ggtcgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa
    33241 atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct
    33301 catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact
    33361 ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc
    33421 catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac
    33481 cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg
    33541 ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcatagtaca tgatgatgtt
    33601 cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac
    33661 gttacggttg atcttaaaca gtgcccaacc gccttgtttt aacttcaggc tttcacgaat
    33721 ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc
    33781 cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac
    33841 cagaataatc agcaggactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc
    33901 gctataactg aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa
    33961 ctggtacata ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatacag
    34021 aggagcggtg taagacgcga tcccgacagc aatgcccagc accacacgag cggcgattaa
    34081 catctctacg ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca ggatggcccc
    34141 cgccatcagg ctgtatttac gacctaggcg gaacgacagc caaccattaa acagcgcacc
    34201 aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac
    34261 aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa
    34321 caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcccgcaa
    34381 agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag
    34441 agtaagacat agtgaaaaaa tacgtgaaca actcacgcag gtgtcaggtc ggaaacagca
    34501 taaatatgga ttaaattgct gcgacatgtc gttatgtgat ggatattcca attttcaaat
    34561 taagttgaat tattgagatt attattaacc acctaat
//
LOCUS       DAZGTR010000039        34521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGTR010000039 DAZGTR010000000
VERSION     DAZGTR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.039"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            198..350
                     /gene="mokC"
                     /locus_tag="ACHLCY_002073"
     CDS             198..350
                     /gene="mokC"
                     /locus_tag="ACHLCY_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP0369331.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(454..1569)
                     /gene="dnaJ"
                     /locus_tag="ACHLCY_002074"
     CDS             complement(454..1569)
                     /gene="dnaJ"
                     /locus_tag="ACHLCY_002074"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP0369332.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGADFSDIFGDVFGDIFGGGRG
                     RQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQTCPTCH
                     GSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPAGVDTG
                     DRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAALGGEIE
                     VPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLNEKQKQ
                     LLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1658..3574)
                     /gene="dnaK"
                     /locus_tag="ACHLCY_002075"
     CDS             complement(1658..3574)
                     /gene="dnaK"
                     /locus_tag="ACHLCY_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP0369333.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3951..4355
                     /gene="yaaI"
                     /locus_tag="ACHLCY_002076"
     CDS             3951..4355
                     /gene="yaaI"
                     /locus_tag="ACHLCY_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP0369334.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4381..5094
                     /gene="msyB"
                     /locus_tag="ACHLCY_002077"
     CDS             4381..5094
                     /gene="msyB"
                     /locus_tag="ACHLCY_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0369335.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5243..5809
                     /gene="satP"
                     /locus_tag="ACHLCY_002078"
     CDS             5243..5809
                     /gene="satP"
                     /locus_tag="ACHLCY_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP0369336.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5844..6431)
                     /gene="mog"
                     /locus_tag="ACHLCY_002079"
     CDS             complement(5844..6431)
                     /gene="mog"
                     /locus_tag="ACHLCY_002079"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP0369337.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6546..7499)
                     /gene="tal"
                     /locus_tag="ACHLCY_002080"
     CDS             complement(6546..7499)
                     /gene="tal"
                     /locus_tag="ACHLCY_002080"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0369338.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7778..9208
                     /gene="yaaJ"
                     /locus_tag="ACHLCY_002081"
     CDS             7778..9208
                     /gene="yaaJ"
                     /locus_tag="ACHLCY_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP0369339.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9278..10054
                     /gene="yaaA"
                     /locus_tag="ACHLCY_002082"
     CDS             9278..10054
                     /gene="yaaA"
                     /locus_tag="ACHLCY_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP0369340.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10208..10504)
                     /gene="yaaX"
                     /locus_tag="ACHLCY_002083"
     CDS             complement(10208..10504)
                     /gene="yaaX"
                     /locus_tag="ACHLCY_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP0369341.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10718..12004)
                     /gene="thrC"
                     /locus_tag="ACHLCY_002084"
     CDS             complement(10718..12004)
                     /gene="thrC"
                     /locus_tag="ACHLCY_002084"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP0369342.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12005..12937)
                     /gene="thrB"
                     /locus_tag="ACHLCY_002085"
     CDS             complement(12005..12937)
                     /gene="thrB"
                     /locus_tag="ACHLCY_002085"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP0369343.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12939..15401)
                     /gene="thrA"
                     /locus_tag="ACHLCY_002086"
     CDS             complement(12939..15401)
                     /gene="thrA"
                     /locus_tag="ACHLCY_002086"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP0369344.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15427..15546)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15482..15547)
                     /gene="thrL"
                     /locus_tag="ACHLCY_002087"
     CDS             complement(15482..15547)
                     /gene="thrL"
                     /locus_tag="ACHLCY_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP0369345.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15761..16447)
                     /gene="yjtD"
                     /locus_tag="ACHLCY_002088"
     CDS             complement(15761..16447)
                     /gene="yjtD"
                     /locus_tag="ACHLCY_002088"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0369346.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16847..16987)
                     /gene="yjjY"
                     /locus_tag="ACHLCY_002089"
     CDS             complement(16847..16987)
                     /gene="yjjY"
                     /locus_tag="ACHLCY_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP0369347.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17083..17799
                     /gene="arcA"
                     /locus_tag="ACHLCY_002090"
     CDS             17083..17799
                     /gene="arcA"
                     /locus_tag="ACHLCY_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP0369348.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17858..19210)
                     /gene="creD"
                     /locus_tag="ACHLCY_002091"
     CDS             complement(17858..19210)
                     /gene="creD"
                     /locus_tag="ACHLCY_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP0369349.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19268..20692)
                     /gene="creC"
                     /locus_tag="ACHLCY_002092"
     CDS             complement(19268..20692)
                     /gene="creC"
                     /locus_tag="ACHLCY_002092"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP0369350.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20692..21381)
                     /gene="creB"
                     /locus_tag="ACHLCY_002093"
     CDS             complement(20692..21381)
                     /gene="creB"
                     /locus_tag="ACHLCY_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP0369351.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21394..21867)
                     /gene="creA"
                     /locus_tag="ACHLCY_002094"
     CDS             complement(21394..21867)
                     /gene="creA"
                     /locus_tag="ACHLCY_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP0369352.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22078..22947
                     /gene="robA"
                     /locus_tag="ACHLCY_002095"
     CDS             22078..22947
                     /gene="robA"
                     /locus_tag="ACHLCY_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP0369353.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22944..23591)
                     /gene="gpmB"
                     /locus_tag="ACHLCY_002096"
     CDS             complement(22944..23591)
                     /gene="gpmB"
                     /locus_tag="ACHLCY_002096"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP0369354.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23643..24164
                     /gene="yjjX"
                     /locus_tag="ACHLCY_002097"
     CDS             23643..24164
                     /gene="yjjX"
                     /locus_tag="ACHLCY_002097"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP0369355.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24250..24576)
                     /gene="trpR"
                     /locus_tag="ACHLCY_002098"
     CDS             complement(24250..24576)
                     /gene="trpR"
                     /locus_tag="ACHLCY_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP0369356.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24666..26603)
                     /gene="sltY"
                     /locus_tag="ACHLCY_002099"
     CDS             complement(24666..26603)
                     /gene="sltY"
                     /locus_tag="ACHLCY_002099"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP0369357.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26814..28481
                     /gene="ettA"
                     /locus_tag="ACHLCY_002100"
     CDS             26814..28481
                     /gene="ettA"
                     /locus_tag="ACHLCY_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP0369358.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28788..30020)
                     /gene="nadR"
                     /locus_tag="ACHLCY_002101"
     CDS             complement(28788..30020)
                     /gene="nadR"
                     /locus_tag="ACHLCY_002101"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP0369359.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30041..31423)
                     /gene="radA"
                     /locus_tag="ACHLCY_002102"
     CDS             complement(30041..31423)
                     /gene="radA"
                     /locus_tag="ACHLCY_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP0369360.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31472..32440)
                     /gene="serB"
                     /locus_tag="ACHLCY_002103"
     CDS             complement(31472..32440)
                     /gene="serB"
                     /locus_tag="ACHLCY_002103"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP0369361.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32546..33190
                     /gene="ytjB"
                     /locus_tag="ACHLCY_002104"
     CDS             32546..33190
                     /gene="ytjB"
                     /locus_tag="ACHLCY_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP0369362.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33218..34234
                     /gene="lplA"
                     /locus_tag="ACHLCY_002105"
     CDS             33218..34234
                     /gene="lplA"
                     /locus_tag="ACHLCY_002105"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP0369363.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34266..34521
                     /locus_tag="ACHLCY_002106"
                     /pseudo
     CDS             34266..34521
                     /locus_tag="ACHLCY_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8142 a         9386 c         8611 g         8382 t
ORIGIN      
        1 agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg tacttgaggc
       61 tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg atattttact
      121 atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact gaccgtaagg
      181 tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg atcgtcatct
      241 gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag gttcacatcc
      301 gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa gtgcaacggc
      361 ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc ctaattttac
      421 ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt caaaaaactt
      481 cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg ttgggccacc
      541 gaagctttct tgcagctctt gcagcagctg tttctgcttc tcgttcaggc ctaccggtgt
      601 ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag acttgacgcc
      661 tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt tcagtttgac
      721 gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag cgaagttgat
      781 cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct gtttaacctg
      841 aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac cttcgcccgc
      901 aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca gcgttttgct
      961 gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca gcgtaccgcg
     1021 gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctgacgca tctgcacctg
     1081 accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg caccgctacc
     1141 gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca cgccacgtac
     1201 agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg ccgcacgttg
     1261 acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat cgctgaagtc
     1321 tgcgccgccg cccataccac cttgctcaaa cgcagcatga ccatactgat cgtatgccgc
     1381 acgtttttgc gagtcggtca gaacttcata agcttccttg atctctttaa atttcgcctc
     1441 ggcctctttg tcaccctggt tacggtccgg gtggtatttc atggccaggc gtttgtaggc
     1501 ctttttgatt tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg
     1561 cttagccatc ttttttaaat tgcccctaga tgaatgcacg ggcggagagg aaattcccct
     1621 tcgcccgtgt cagtataatt acccgtttat agggcgatta ttttttgtct ttgacttctt
     1681 caaattcagc gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg
     1741 cagtctgctg ctgggcatgt tgctgctggg cgatttccat cagtttctgg gaaacctgtg
     1801 ccagctcctg cattttcgct tcgatagcgg ctttgtcttc acctttcaga gcagtttcca
     1861 gtgcagtcag tgcagactcg atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt
     1921 cttcaacctg cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca
     1981 gctcttcaaa cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc attttctgga
     2041 tttcatcttc gttcagacca gaagaagcct tgatggtgat cttctgctct ttaccgctgt
     2101 ttttatcttt cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag gtaacttcga
     2161 tctgcggcat gccgcgcggt gccgggttga taccatccag gttgaactga cccagagatt
     2221 tgttatcagc cgcacgttta cgttcaccct gcagcacatg gatggttacc gcagactggt
     2281 tgtcttcagc ggtagagaac acctggctgt gcttggtcgg gatagtggtg tttttcgcga
     2341 tcagcgtcgt catcacaccg cccatggttt cgatacccag agacagcggg gtaacgtcca
     2401 gcagcagtac gtctttcacg tcaccagtca gaacaccccc ctgaacagca gcaccgattg
     2461 ctacagcttc gtccgggtta acgtctttac gcggctcttt accaaagaac tcagcaactt
     2521 tcttctgaac cattggcata cgagtctgac caccaacgag gataacgtcg tcgatatcag
     2581 atacggacag gccagcgtcc tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca
     2641 gatcttcaac caggctttcc agtttcgcac gagtcacttt gatgttcatg tgtttcggac
     2701 cggtcgcatc tgcagtgatg tacggcaggt taacgtcggt ctgctgagcg gaagacagtt
     2761 cgattttcgc tttttctgcc gcttctttca ggcgctgcat tgccagcgga tcgttgcgca
     2821 ggtcaatgcc ctgatctttc ttgaattctt caaccaggta gttgatcaga cggctgtcga
     2881 agtcttcacc acccaggtgg gtatcaccgt tggtagccag aacttcgaag gttttttcgc
     2941 cgtcaacttc gtcgatttcg ataatagaaa tatcgaaagt accaccaccc aggtcataaa
     3001 ccgcgatagt acggttgcca gtacctttgt ccagaccgta agccagcgca gctgcggtcg
     3061 gttcgttgat gatacgtttt acttccagac cagcgatacg gcctgcgtct ttggttgcct
     3121 gacgctgagc atcgttaaag tatgccggta cggtgataac agcttcagtt accggttcac
     3181 ccaagtaatc ttcagcggtt ttcttcattt ttttcagcac ttcagcagaa atctgcggcg
     3241 gtgccatttt ctggccttta acttcgaccc atgcgtcgcc gttatcagca gcaataattt
     3301 tgaacggcat gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg cggccaatca
     3361 ggcgtttaat cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct
     3421 gaccaaccag agtttcacca tcctgggtat aggcaataat agaaggcgtg gtgcgatcgc
     3481 cttcggcgtt ctccagcacg cgaggagtgg tgccatccat aatcgctaca caagagttgg
     3541 tagtacccag gtcgatacca attattttac ccatctaaac gtctccacta tatattcggt
     3601 catcatgtgg ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact gcccaatttc
     3661 atttttttgc agactcactc actgcggttg actactaaat ggggtcgtaa cccacgtcat
     3721 caagggggag atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga tatagcagga
     3781 caagacgtga aactgacagc aattttattc atataaagaa tgaatcgcag ccaagacatt
     3841 catcaaaaaa ttgtaataaa aagaaaacat tacgcacctg aatcttctct ttatcagcag
     3901 taaactagtg ggtatccatc cccctacctc tccccactaa gagaatcctt atgaaatccg
     3961 tttttacgat ttccgccagc ctggcgatta gtctgatgct gtgctgcacg gcgcaggcaa
     4021 acgaccataa aatcctcggc gtcattgcga tgccacgtaa cgaaaccaac gatctggcgc
     4081 tgaaacttcc tgtttgccga attgttaaac gcatacaact ctctgccgac catggcgatt
     4141 tacagttaag cggtgcatcg gtttatttca aagccgcccg tagcgccagt cagagcctga
     4201 atattccttc agaaataaaa gaagggcaaa ccactgactg gatcaacatt aacagcgata
     4261 acgacaataa acgctgcgtc tcaaaaatca ccttttccgg tcatacggtg aactcatcgg
     4321 atatggccac gctgaaaatt atcggcgacg actaacgcca gaatatagaa gccacaaaaa
     4381 atgaatgtta attacctgaa tgattcagat ctggattttc tccagcattg cagtgaggaa
     4441 cagttggcaa atttcgcccg attgctcacc cataatgaaa aaggcaaaac tcgcctctcc
     4501 agcgtactga tgcgcaacga actgtttaaa tctatggaag ggcatcccga gcaacatcgc
     4561 cgcaactggc agctgattgc tggagaatta cagcattttg gtggcgatag tatcgccaac
     4621 aaactgcgcg gacacggtaa attgtatcgg gccattttgc tcgatgtttc aaaacgattg
     4681 aagctgaaag ccgacaaaga gatgtctacg tttgagattg agcaacagtt actggaacaa
     4741 tttctgcgta acacctggaa gaatatggac gaggaacata aacaggagtt tctgcacgcg
     4801 gtcgatgcga gggtgaatga gctggaagag ctgctgccgc tgctgatgaa agacaaatta
     4861 ttggcaaaag gcgtgtcgca tttgctttcc agccaactga cccgcatttt acgcacccac
     4921 gcagcaatga gcgtacttgg gcatggtttg ctgcgcggcg cggggctggg aggccctgta
     4981 ggtgcggcac taaatggggt taaagcggtc agcggcagcg cctatcgcgt gacgattcca
     5041 gccgtactgc aaatcgcctg cctgcgccgg atggttagcg ccactcaggt ctgataccgg
     5101 aaaccgggga aaattttcat tttcccctca aaagatcgta gacactgccc cactggctga
     5161 ttattatgcc gcgccctgaa aacactacag ttattcaggg aaattatttc accattcatt
     5221 cgatgatgat ttttgaggaa ttatgggcaa cactaagttg gctaatccgg caccgctggg
     5281 cctgatgggc ttcggcatga ccaccattct gcttaacctg cacaacgtgg gttatttcgc
     5341 tctggacggt attattcttg ccatgggcat tttctacggc ggcatcgcgc aaatttttgc
     5401 tggtctgctg gagtacaaaa aaggcaacac tttcggttta accgcattca cctcttacgg
     5461 ttctttctgg ctgacgctgg ttgcgattct gctgatgccg aaactgggtc tgaccgatgc
     5521 gccaaatgca cagttccttg gtgtctatct gggtctgtgg ggcgtattta cgctgtttat
     5581 gttcttcggc acgctgaaag gcgcacgcgt tctgcaattc gttttcttta gcctgaccgt
     5641 gctgtttgcc ctgctggcga tcggtaacat tgccggtaac gccgcaatca tccactttgc
     5701 cggctggatt gggctgatct gcggtgccag cgcaatctat ctggcgatgg gtgaagtact
     5761 gaacgagcag tttggtcgca ccgttctgcc gattggtgaa tcccactaat tgttttgaga
     5821 tccccccgct cggggggatt tttttattcg ctaacgtcgc gtcttgcact cttcggtctg
     5881 aatgctgcaa ccacttccgg tgccgtttca acgtatggcc cttccagcaa ctgaatgcag
     5941 tacggtacgc tggcaaaaat accgtgtacc acaacgttac ccgcagcgtc cttcacacct
     6001 tccagcgtct ctttaataga cttcggctga ccgggtaagt taaggatcag cgcctgtttg
     6061 cgaatcacac caacctgacg cgaaaggatc gcagttggta caaaatgcag gcttatctgg
     6121 cgcatctgtt caccaaagcc aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc
     6181 gttacgtcac gacgcgccgg gccagttccg cccgtggtga gcaccagatg gcaactcatt
     6241 tcatccacta gctcacacaa cgtttgctcg atgatcgcct gctcatcggg gattaagcgg
     6301 gtttccagtt caaacggcgt ggttagcgcc gatgtcagcc attcttccag cgcagggatg
     6361 cctttatcct gataaacgcc gctggatgcg cgatcagaga tggaaactaa gccaatacgt
     6421 aaagtattca tcaagatttt tccgtttaaa cagtctcgtt aaacagaatg atacactgcg
     6481 aagggagtga cagacaggct tcagaagagg tagcgtgacc gacttcccgg tcacgctaag
     6541 aatgattaca gcagatcgcc gatcattttt tccagttttt cctggtcaat agcaaactta
     6601 cggatacctt ccgccagttt atctactgcc attggatcct ggttgtgctg ccacaggaac
     6661 tcggactcag tgatacgcgc cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt
     6721 tcgatagccc cttcgctctc agccagctct ttcagcagtg ccggtgcgat ggtcagacgg
     6781 tcgcagcctg ccagttccag aatttcgccg atgttacgga agcttgcgcc cataaccacg
     6841 gtttcataac cgtgctcttt gtagtactgg tagatttcag atacagaaac cacgcccgga
     6901 tcttctgccg gagcgtactc tttcttatcg gtattcgctt tgtaccagtc aagaatacgg
     6961 ccaacaaacg gcgagatcag gaacacgccc gcttccgcac aagcacgagc ctgagcgaag
     7021 gagaacagca gggtcaggtt acagttgatg ccttcttttt ccagctgttc tgcagcacgg
     7081 ataccctgcc aggtagaagc cagtttgatc agaatacgat cgttgctaat accagcatcg
     7141 ttgtagagtt tgatcaggcg ttttgctttc gcaattgacg cttcagtgtc ataggaaaga
     7201 cgcgcatcaa cttcagttga gatacggccc ggaaccagtt tcaggatttc cagaccaata
     7261 tttactgcca gtttgtcggt cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt
     7321 ttcgcccagg cgacagcatc atcaatcaac ttacggtatt ccggaatctg cgctgcgtta
     7381 agaatgagag aagggttggt tgtggcatcc tgcggttgat acagcttcat tgccgcgatg
     7441 tccccagtgt cggccactac ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg
     7501 atagtatttc tctttaaaca gcttgttagg gggatgtaac cggtctgccc tgatgatatc
     7561 acgacgcaac gtcagcgcaa ccgccgacaa gacggttatc gccaggcgag actgtttcgg
     7621 atttctgaca ctgcgttgat atcgcccgcc atttttatac aaaacctcat gtatgctacg
     7681 cagaagttat caagtacctc gtagcgtata tacttcttaa acaattggta acgtttacac
     7741 aggaaagtca tcgcgaccgg caataagagg gatatgcatg ccagattttt tctcctttat
     7801 taacagcgtc ctttggggat cggtaatgat ttacctgctc ttcggcgcag gttgttggtt
     7861 cacttttcgc accggatttg tgcagtttcg ctacatccgc cagtttggca aaagtcttaa
     7921 aaatagcatt catccacagc caggcggttt aacctcattt cagtcattgt gtaccagtct
     7981 tgcggcgcgc gtgggtagcg gcaacctggc cggcgttgcg ctggcaatta ccgccggtgg
     8041 acctggagcc gtcttttgga tgtgggttgc cgcgtttatc ggcatggcga cctcgtttgc
     8101 cgaatgttct ctggcacaac tttataaaga acgtgacgtc aatggacagt ttcgtggcgg
     8161 accggcatgg tatatggcgc gcgggctggg gatgcgttgg atgggcgttc tgttcgccgt
     8221 ctttttgctc atcgcctatg gcataatttt cagcggagtt caggcgaacg ccgttgcccg
     8281 cgccctaagt ttttcttttg attttcctcc gctggtgaca ggcattattc tcgctgtctt
     8341 tgctctgctg gcaatcactc gcggtcttca tggcgtcgcc cggctcatgc agggttttgt
     8401 cccgttgatg gcgataatct gggtactgac cagcctggtg atttgcgtaa tgaatatcgg
     8461 gcaacttccc cacgtcattt ggtctatttt tgagagtgct tttggctggc aggaagcggc
     8521 aggcggcgcg gcgggatata ccttaagcca ggcgattact aacggttttc agcgcagtat
     8581 gttttccaat gaggcgggaa tgggttctac accaaacgca gcagcggcag cggcgtcctg
     8641 gcctccgcat ccggcagcgc aagggattgt ccagatgatt ggcattttta tcgacaccct
     8701 ggtcatctgt acggcaagcg ccatgctgat attactggcg ggtaacggca caacctacat
     8761 gccgctggaa ggtattcagc ttatccagaa ggcgatgcgg gtgctaatgg gttcctgggg
     8821 tgctgagttt gttaccctcg tggttattct gtttgccttc agctccatcg ttgccaacta
     8881 catttatgcc gaaaacaatc tcttcttttt acgcctgaac aaccctaaag cgatctggtg
     8941 tttgcggatc tgcaccttcg caacggtcat cggcggcacc ttgctaagtc ttccgctgat
     9001 gtggcaactg gcagatatca taatggcctg catggctatt accaatttga ccgccatttt
     9061 actgctctcg cctgtggttc ataccattgc cagtgattat ctacgccagc gtaaactcgg
     9121 cgtgcgcccg gtgtttgatc cgttgcgtta tccggatatc ggtcgccagc tttctccgga
     9181 cgcgtgggat gatgtttcgc aggagtaatc acaactatcg atcaactcat tctctttttt
     9241 tgctaaagtc ggcataaatt tcctgcaagg actggatatg ctgattctta tttcacctgc
     9301 gaaaacgctt gattaccaaa gcccgttgac caccatgcgc tatacgctgc cggagctgtt
     9361 agacaattcc cagcagttga tccatgaagc gcggaaactg acgcctccgc agattagcac
     9421 gctgatgcgc atcagcgaca aactggcggg tatcaacgcc gctcgctttc atgactggca
     9481 gccagatttc acgccggaga atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta
     9541 caccggcttg caggccgaaa ccttcagcga agacgatttc gattttgccc aacagcattt
     9601 gcgaatgctt tccggcttgt atggcgtact gcgcccgctc gatttaatgc agccttatcg
     9661 tctggaaatg gggatccgtc ttgagaatgc ccgagggaaa gatctgtatc aattctgggg
     9721 agatatcatc accaacaagc tgaacgaggc gctcgcagca caaggcgata atgtggtgat
     9781 taatctggcg tcagatgagt attttaaatc ggtgaagccg aagaaattga atgccgagat
     9841 tatcaagccg gtgttcctcg atgagaagaa cggcaaattt aagatcatca gcttctacgc
     9901 taagaaagca cgcggtctga tgagtcgttt cattattgaa aatcggctga ccaaaccaga
     9961 gcaactgact ggttttaata gcgaaggtta cttctttgat gaagattcct ccagcaatgg
    10021 cgaactggtg tttaaacgct acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg
    10081 tgaacgcctt atccggccta caaaagcatg cagattcaat atattgcaga attcacgtag
    10141 atcctgataa gacgcgccag cgtcgcatca ggcgctgaat gccggattgt tacccggcat
    10201 ttgtcattta gcggtgatgt ttgcctggac catgaccgcc gtgatgatca tgaggagctt
    10261 tcttatggtg gcgcggcggt ggcggcggtc cgtgtgggtg ccagcgattg cctcgccatt
    10321 cataatgttg tttccaccag ccgtggtcgc gccagtgacc tccatcccag taatagccac
    10381 gattatcacg atcgcctatc tgtaatttta ctgacgggac taacgtaatt tccgcagcct
    10441 gtgctgccat gggagcgacc agaaccaggg aaagtgcgag tacgatagat tgcatctttt
    10501 tcacaggtta ctccttttat cattgcccgt tatgggtcga tatccggaga ttacgcgatg
    10561 agaacgacgg ttgttattct ccgcaatcct aatctctaag ttaccgcatt tattgagaat
    10621 ttctcctttt tccctgtcgt ttttctccat aatttcttca taaaaaagcc gggctgcata
    10681 aaagcaaacc cggcctgatt gagataatga atagatttta ctgatgattc atcatcaatt
    10741 tacgcaacgc agcaaaatcg gcgggcagat tatgtgaaag caagggtaaa tcagcacgtt
    10801 ctgccagctc ttttggcaga tccaacgttt caccgagaat cgcttccacg ctctctttaa
    10861 atttcgccgg atgcgcggtg ccgaggaaca agccatattc gcctggattc aactggtcac
    10921 gcagcgcacg atacgctacg gcagcgtgcg gctccgaggt gtagcccagt tcttttaact
    10981 cacgcattgt ctgttgcgtg gtttcatcat ccacggctgc ataacccagc tctttcagtt
    11041 gccagatttt gcggcggaac aactcttcca cacgcggcca gttgttcggc tgactcacgt
    11101 ccatcgcgtt ggataacgtc gcctgagtcg ctttgggtga ccactgaccg tcgtgcagga
    11161 aacgtggcac ggtatcgttc acgttggtcg cagcaataaa acgtttcacc ggcagaccga
    11221 gtgacttcgc cagcagaccc gccgtcaaat cgccgaagtt tccgcttggc accgagacaa
    11281 ccagctggtt gcgcgcttcc tgcggcagct gcgcaacagc ttcaaagtag tagcaaatct
    11341 gcgccagcaa acggctgatg ttaatcgagt tagctgagtt taaccccagc gccactttca
    11401 gttcttcatc atcaaacgcc tgcttcacca gcgcctgaca ggcatcgaaa tcgccgtcga
    11461 tggcaacagt ttcgatattg ccgcccaacg tacagaacag tttttcttgc agtggactga
    11521 ttttgcctcg tggatagagg ataaccactt tcacattcgg taaaccgtag aaagcatgag
    11581 ccactgccgc tccggtatca ccggaggtcg cggtcagaat ggtcactggc ttatcgcccg
    11641 caatatgggt cagcatttgt gccataaagc gaccgccgaa atctttaaat gccagcgttg
    11701 gcccgtggaa caattccaga caaccgacat cgctttcaac attggcgacc ggagccggga
    11761 aggcaaacgc cgcgcgcacg cgctcttcca ggatttcctg cgggatttca tcaccaataa
    11821 acgccgagag gatcttcgca ctgcgggtga caaaatccag cttcagcatc tcatcaattt
    11881 cagtcaggct gaattccggc aggtcatgcg gaaaaaacag cccctgattt ttgcccaacc
    11941 cctgggttac ggcttgcgca aagctgacct gctcgttgtg atctttcaga ttgtagagtt
    12001 tcatttagtt ttccagtact cgtgcgcccg ccgtatccag ccggcaaata tgaacaaaac
    12061 cttcctgatt ttgcaggtag ttcttaccca accagtcggc aacgcgctgg gcggtatccg
    12121 gcttgtcaca tagagcgaac aaggtcgggc cggagccgga gataccgctc gctaccgcgc
    12181 cgatttccgc gaccgcctgc cgcgcctgcc ggaagccagg cagtaaccgt tcacggtagg
    12241 gttcagcgat aacatctttc atcagcttcg cggcaagctc aggctgacgg gaatagcagg
    12301 cgtgaatgaa gccagccaga tgtcgcccgt gcgcaatgca atcctggcgg cgatactgcg
    12361 ccggtaaaat agccctggct tctgccgtcg agactttaat ccccggatac gccagcaccc
    12421 acagccactc atcaaaccct ggcacttgct ggctgatgat gtcgttttct tcgatcatca
    12481 actgcatacc accaagaaaa cacggtgcca cgttgtcgta atgaatgctg ccggagattc
    12541 gtccttccag ctcgcccatc aaagccagca aacgagtgtc attaagcggc ttgccacagt
    12601 gttcattcat cgccatcagc gccgcgacca ccgaacaggc gctggagcct aagcccgaac
    12661 cgattggcat attcttttcc agagtcatcg ccactggaat ttgcttgcca agctcctggc
    12721 aaaaacgctc ccagcactga taaacgatat tttcccgtgg ttctgacggc agcttatcgg
    12781 caaagcgtcc gaggttgttg agactgaatg tctctgccgc ctcaaccgtg actacatctc
    12841 cgagcaatgc accatcaaca ggtgtcaccg ccgccccgag cacatcaaac ccgacgctca
    12901 tattggcact ggaagccggg gcataaactt taaccatgtc agactcctaa cttccatgag
    12961 agggtacgta gcagatcggc aaagacaccg gcagctgtaa cgtcattgcc cgcaccatat
    13021 ccgcgcagca ccaacggcag cggctgataa tagtggctat aaaaggccag ggcgttttcg
    13081 ccatttttca ctttgaacag cggatcatta ccatccactt cggcaatctt cacgcggcag
    13141 gcaccatctt catcaatatt gccaacatag cgcaaaactt ttccttcatc acgggccttc
    13201 gccacgcgcg cggcaaagag atcgtcgagc tgtgacagat tcgccataaa agcggcaaca
    13261 tcaccctcag cgttaaactc tgcgggcagc acaggttcaa tttcaatatc cgccagctcc
    13321 agttcacgtc ccgtttcacg agcgagaatc aatagcttac gcgctacatc cataccagaa
    13381 agatcatctc gcggatccgg ttcggtataa cccatttccc gcgccagagt agtcgcctcg
    13441 gagaaactca tgccttcgtc taacttgccg aagatataag aaagcgaacc tgaaagaatg
    13501 ccggagaact tcatcaattc atcaccagca ttgagcagat tttgcaggtt ctcaataacc
    13561 ggtaatccag ccccaacgtt ggtgtcatag aggaatttac gccgcgattt ttccgccgca
    13621 tgacgcaaca gatggtagta atccatcgac gaggtgttgg cctttttgtt cggcgtgaca
    13681 acgtggaaac cttcgcgcaa gaagtcggca tattgatccg ccactgcctg gctggaggtg
    13741 cagtcaacaa tgaccgggtt cagcagatga tattctttca cgaggcgaat taagcgcccg
    13801 agattaaacg gctctttggc ttgcgccagt tcttcctgcc agttttccag atttaggcca
    13861 tgcacattgg tgagcagtgc cttcgagttg gcaacaccgc agacacgtaa gtcgatatgt
    13921 ttattcttca accagctttg ttgacgcttc agttgctcca gcagcgcacc gccaacgcca
    13981 ccgacgccaa tcacaaacac ttcgataacc tgatcggtat tgaacagcat ctgatgagta
    14041 acgcgcacgc cagtggtcgc atcatcgtta tttaccacga cagagattga gcgttcagaa
    14101 gatccctgag caatggcgac aatgttgata ttggcgcggg ccagcgcggc aaagaatttc
    14161 gccgagatcc cacgcaaggt gcgcatacca tcacctacca ccgagataat ggccagccgt
    14221 tccgtcaccg ccagcggctc cagtaagcct tctttcagtt ccaggtagaa ctcttcctgc
    14281 attgcccgtt cagcccgtac acagtcgctt tgcggaacgc agaaactgat actgtattcg
    14341 gaagatgatt gcgtaatcag caccacggaa atacgggcgc gtgacatcgc ggcaaagacg
    14401 cgcgccgcca tgccgaccat tcctttcatc cccgggccgg aaacgctgaa cattgccatg
    14461 ttattcagat tggaaatgcc cttgaccggt aattcgtctt catcacgact ggcaccaatg
    14521 agcgtacctg gtgcttgagg atttccggta tttttaatca ggcaagggat ctggaactgg
    14581 gcgatggggg taatggtgcg ggggtgaaga actttagcgc cgaagtagga aagctccatc
    14641 gcttcctggt aggacatcga cttcaacaac ctcgcatcgg gcacctgacg cgggtcgcag
    14701 gtatagaccc cgtcaacgtc cgtccaaatc tcgcaacaat cggcgcgtaa acaggcagcc
    14761 aacaccgccg cggagtagtc ggaaccgtta cgtccaagta ccaccagttc gcctttttca
    14821 ttaccggcgg tgaagcctgc catcagcacc atgtgatcag ccggaatacg acttgccgca
    14881 atacggcggg tggactcagc aatatcgacg gtagattcga ggtaatgccc cactgccagt
    14941 agtttttcga ccggatcgat aacggtaacg ttgtgaccac gcgcttctaa cacgccggcc
    15001 ataatggcga tcgacatttt ctcgccacgg caaatcagcg cagcgttgat gctatccggg
    15061 cactgcccca acaaactaat gccatgcagg acatgtttta tttgggcaaa ttcctgatcg
    15121 acgaaagttt tcaattgcgc cagcgggaac cccggctggg cggctgcgag tcccgtcaaa
    15181 agttcggcaa aaatacgttc ggcatcgctg atattgggta aagcatcctg gccgctaatg
    15241 gttttttcaa tcatcgccac caggtggttg gtgattttgg cgggggcaga gaggacggtg
    15301 gccacctgcc cctgcctggc attgctttcc agaatatcgg caacacgcag aaaacgttct
    15361 gcatttgcca ctgatgtacc gccgaacttc aacactcgca tggttgttac ctcgttacct
    15421 ttggtcgaaa aaaaagcccg cactgtcagg tgcgggcttt tttctgtgtt tcctgtacgc
    15481 gtcagcccgc accgttacct gtggtaatgg tgatggtggt ggtaatggtg gtgctaatgc
    15541 gtttcatgga tgttgtgtac tctgtaattt ttatctgtct gtgcgctatg cctatattgg
    15601 ttaaagtatt tagtgaccta agtcaataaa attttaattt actcacggca ggtaaccagt
    15661 tcagaagctg ctatcagaca ctcttttttt aatccacaca gagacatatt gcccgttgca
    15721 gtcagaatga aaagctgaaa aatacttact aaggcgtttt ttatttggtg atattttttt
    15781 caatatcatg cagcaaacgg tgcaacattg ccgtgtctcg ttgctctaaa agccccaggc
    15841 gttgctgtaa ccagtcgacc agttttatgt catctgccac tgccagagtc gtcagcaatg
    15901 ccatgactcg ttcgcgtaaa gcttgcagtt gatgttggtc tgccgttgtg tcacttttcg
    15961 tcggttgttg tattaatgtt gctaattgat agcaatagac catcactgcc tgccccagat
    16021 tgagcgaagg ataatccgcc accatcggca caccagtaag aacgtcagcc aacgctaact
    16081 cttcgttagt cagcccggaa tcttcgcgac caaacaccag cgcggcatgg ctcatccatg
    16141 aagatttttc ctctaacagc ggcaccagtt ctactggcgt ggcgtagtaa tgatatttcg
    16201 cccgactgcg cgcagtggtg gcgacagtga aatcgacatc gtgtaacgat tcaaccagtg
    16261 tcgggaaaac tttaatatta tcaataatat caccagatcc atgtgcgacc cagcgagtgg
    16321 ctggctccag atgtgcctga ctatcgacaa tccgcagttc gctaaacccc atcgttttca
    16381 ttgcccgcgc cgctgcccca atattttctg ctctggcggg tgcgaccaga ataatcgtta
    16441 tacgcatatt gccactcttc ttgaccaaat gaccgcgaac agggtgatca ctgtcaactt
    16501 attacgcggt gcgaatttac aaattcttaa cgtaagtcgc agaaaaagtc ctttacttag
    16561 cttaaaaaag gctaaactat ttcctgactg tactaacggt ttaattgtta aaaaatgcta
    16621 catatccttc tgtttactta ggataatttt ataaaaaata aatctcgaca attggattca
    16681 ccacgtttat tagttgtatg atgcaactag ttggattatt aaaataatgt gacgaaagct
    16741 agcatttaga tacgatgatt tcatcaaact gttaacgtgc tacaattgaa cttgatatat
    16801 gtcaacgaag cgtagtttta ttgggtgtcc ggcccctctt agcctgttat gttgctgtta
    16861 aaatggttag gatgacagcc gtttttgaca ctgtcgggtc ctgagggaaa gtacccacga
    16921 ccaagctaat gatgttgttg acgttgatgg aaagtgcatc aagaacgcaa ttacgtactt
    16981 tagtcatgtt acgccgatca tgttaatttg cagcatgcat caggcaggtc agggactttt
    17041 gtacttcctg tttcgattta gttggcaatt taggtagcaa acatgcagac cccgcacatt
    17101 cttatcgttg aagacgagtt ggtaacacgc aacacgttga aaagtatttt cgaagcggaa
    17161 ggctatgatg ttttcgaagc gacagatggc gcggaaatgc atcagatcct ctctgaatat
    17221 gacatcaacc tggtgatcat ggatatcaat ctgccgggta agaacggtct tctgttagcg
    17281 cgtgaactgc gcgagcaggc gaatgttgcg ttgatgttcc tgactggccg tgacaacgaa
    17341 gtcgataaaa ttctcggcct cgaaatcggt gcagatgact acatcaccaa accgttcaac
    17401 ccgcgtgaac tgacgattcg tgcacgcaac ctgctgtccc gtaccatgaa tctgggtact
    17461 gtcagcgaag aacgtcgtag cgttgaaagc tacaagttca atggttggga actggacatc
    17521 aacagccgtt cgttgatcgg ccctgatggc gagcagtaca agctgccgcg cagcgagttc
    17581 cgcgccatgc ttcacttctg tgaaaaccca ggcaaaattc agtctcgtgc tgaactgctg
    17641 aagaaaatga ccggccgtga gctgaaaccg cacgaccgta ctgtagacgt gacgatccgc
    17701 cgtattcgta aacatttcga atctacgccg gatacgccgg aaatcatcgc caccattcac
    17761 ggtgaaggtt atcgcttctg cggtgatctg gaagattaat cggctttacc accgtcaaaa
    17821 aaacggcgct ttttagcgcc gtttttattt ttcaacctta tttccagata cgtaattcat
    17881 cgtccgttgt aacttcttta ctggctttca ttttcggcag tgaaaacgca taccagtcga
    17941 tattacgagt cacaaacatc atgccggcca gcgccaccac cagcacactg gttcccaaca
    18001 acagcgcgct atcggcagag ttgagcagtc cccacatcac accatccagc aacaacagcg
    18061 cgagggtaaa caacatgctg ttgcgccagc ctttcaatac cgcctgcaaa taaataccgt
    18121 tcattaacgc cccaatcaga ctggcgatta tccatgccac ggtaaaaccg atatgttcag
    18181 aaagcgccag caagagcaaa taaaacatca ccaatgaaag ccccaccagc aaatattgca
    18241 ttgggtgtaa acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata aaagtcagtg
    18301 caatcagcag aatggcgtac ttagtcgccc ggtccgttaa ttggtattga tcggctggcg
    18361 tcgtcactgc gacgctaaac gcggggaagt tttcccagcc ggtatcattg cctgaagcaa
    18421 aacgctcacc gagattatta gcaaaccagc tgctttgcca ttgcgcctga aaacctgact
    18481 cgctaacttc ccgtttggct ggtagaaaat cacctaaaaa actgggatgc ggccagttgc
    18541 tggttaaggt catttcgcta ttacgcccgg caggcaccac agaaagatcg ccggtaccgc
    18601 ttaaattcag tgccatattc agctgcaggt tctgcttccg ccagtcccct tcgggtaaag
    18661 ggatatgcac gccctgcccg ccttgctcta acccggtacc gggttcaatg gtcagtgccg
    18721 ttccgttaac ttcaggcgct ttcaccacac caataccacg cgcatccccg acgctaatca
    18781 caataaaggg cttgcctaag atgatatttg gcgcgttgag ctcgctaaga cgcgaaacat
    18841 cgaaatcggc ttttagcgtt aaatcactgt gccagacctg accggtataa atccctatct
    18901 tgcgttcttc cacgttctga ttgccatcaa ccatcaatga ctcaggtaac caaaaatgga
    18961 taaaacttcg tttccgctcc acggttttat cctcttcctg caccgtataa agctcggtca
    19021 caggaatagc gataagcggc ccaacgagtt tttgcggccc gctggtactt tggcgaatcg
    19081 catcttccac atcgctacgg taatcggcac gttcgacaat cacctgccga atcagcatta
    19141 tcggaatcaa caacagcaat actgcaccaa acagggtggt cattttccag aacagggggg
    19201 atttcaacat agtcttctcc tttgcaatgg cagcaggata cgaaggctat gtgggaagaa
    19261 tttgaagcta tgtgaagtga cggtgaagtc gaagcgaggc cagcacgcca ccttcctgca
    19321 cgttgcgcag cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg aacgccaacc
    19381 ccagaccgct gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt tcaaaaatac
    19441 gtgacagcgc gtagtcagga ataccactac cggtatccag caccttaagc gtgacgtgtt
    19501 cctgatccac ttcggcgctt agcgttatac gaccgctctc gggggtaaaa tcgatggcgt
    19561 tatccagtaa attccccagc gcctgatcca gtaacgccgg ttcagcagca acattaacct
    19621 cggtgggcgt aacatgtaga gtgatgtttt tttctgccaa ctgcacggtg cgcgcttcgc
    19681 tgacgcggcg gaataatgcc gccacatcaa cagcagtcaa aacgacttcc tgacgattct
    19741 ccagtcttgc ctggcgtagt aacgtttcca ccagtgcctg cattcgcgca ttttgcgtca
    19801 gaatgttgtc agtaaaacga gccactactt caggcggcgg accttcgcgt aaaatttccg
    19861 ccgcgccacg aatcgccgcc agtgggcttt ttagctcatg ggttaacgca taaacatact
    19921 gctcaatata gtttttccct tccagcttca cgcgcatact ttccagcgcc tgcgcgagtt
    19981 tacgcagctc gctactaccg agatcgggaa gaggaacggg cttattgtca gtgacggaat
    20041 cagcatagcg agtgagccgg gcaatggagc ggttgatcca ccaaaccatg cctgcgccaa
    20101 tcaccagtgc gatccccaac aaaatggcgc tggcccataa aattcgccgc tcgctgcgtt
    20161 taatgaccgg agccatcgcc gcgttcggtt tgcctacgct caaaacgcca ataagccgcg
    20221 agccgtccat aatcggcgcg gcaacataca tcaccgaact ttcgggatcg gcaggatttt
    20281 gcaacgtgct gcgcgcacca tactgaccac gcaacgttag ccagacgtca ttccagcgcg
    20341 aataatcctg cccaacggct ttatttgccg aatcgaacaa tactttaccc tgcgaatcgg
    20401 tcatatagac atgatattca ttgcgcactt tgttaatgcc accgatattg gcgcgaaacg
    20461 ggcgatgttg tagctgatta aacgcctgcg ccagttgccc atgcgttggg tccccagaga
    20521 gcaaatcggg acgcgccagc tccgccagca acgttgcggt gtcgatcaac gtcccctccg
    20581 ttgctcttcg cacgcccggt ttaacttctt tgacaaaaat agccagtacg aaccaggccg
    20641 ccaccgccac cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc attacaggcc
    20701 cctcaggcta tatcccatgc cgcgatgagt attaatcggt gaaagatcgg ggttgatggc
    20761 gcgcagcttg gcacgcaacg ttttaatgtg ggtatcaacg gtgcgatcgt aggtgtcctg
    20821 cgcatcttcc cacacgctat ccatcagttg ctggcgggac cagacgcggc ctggcgactt
    20881 gagtaacgtc ttcagcaata aaaactcata ccgcgtcagc gttaatggcg tgtcaaacca
    20941 gctgatctgc gccgcgggtt cattcaattc aaaatgacca atacggatga cgggagacgg
    21001 cgacgagaac ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca cttcgcgggg
    21061 tgaaaacggt ttagccacat agtcgtcagc gccaatttcc agcccaagca ggcgatcgac
    21121 ctcttcactt cgggccgtca ggaacagtac aggtaacgcc ggatggagcg ccagtaactg
    21181 gcggcacaat tcaaagccgc tgatatcagg caaaccaacg tcgagaatca tgacgtcggg
    21241 aaccagctgg cgagctttat ccagcaccgg caagcctcgc tcaaagacct cgacggcaaa
    21301 accttcctgc tgcaacatgt agaccagcgt gtcggctatc ccttgctcat cttccactaa
    21361 ccagaccgtt tcccgttgca taaatcgcct ctgttattgc cgccacggca tgacaggaac
    21421 cgcgctaatc gcgtttttcg gcgaaccttc tacaactttg tcggagtaag ccagataagc
    21481 gagcgcgttg cgtttggcgt cataaaagcg cacgacctgt aacgacttaa agaccaggga
    21541 cgtgcgtttt ttgaatacta cctcgccctg agctttgccg tttttaatac gatccgacag
    21601 ttcaatcggc ccgacttgct gacaagaaat ggccgcatcg gaggtatctt ccgccaggcc
    21661 caatccccct ttaatacccc cggttttcgc ccggctcaca taacaggtga catttttcac
    21721 atcgggatcg tcaaaggctt ccacaacaat tttgtgatcc gggccgatca ttttaaatac
    21781 ggtgtcgacc gaaccaatct cttctgcatg agccaatggc cccagcatta tcaggcttaa
    21841 agacaggatc aaatgcttgt atttcatatt gttaccattt ttatctatta cacgagcaat
    21901 tagttcgtca cggtaaataa catattgatt aagaacaaaa tctcaatact tttatttccg
    21961 tcaagcccta aaacatactc tactaagaaa aaaacactgc atgctaaaac agcaaaaaat
    22021 gctattatcc aattacctga tgtcaggtgc tcgttgttga aaggatgagg atattttatg
    22081 gatcaggccg gcattattcg cgacctttta atctggctgg aaggtcatct ggatcagccc
    22141 ctgtcgctcg acaatgtagc ggcgaaagca ggttattcca agtggcactt acagagaatg
    22201 tttaaagatg tcactggcca tgctattggc gcgtatattc gtgctcgtcg tttgtcgaaa
    22261 tcggcggtcg cactacgcct gactgcgcgt ccgattctgg acatcgcgct gcaataccgc
    22321 ttcgactctc aacagacatt tacccgcgca ttcaagaagc agtttgccca gactcctgca
    22381 ctttaccgcc gttctcctga atggagcgcc tttggtattc gcccgccgct acgtctgggt
    22441 gaattcacaa tgccagagca caaatttgtc accctggaag atacgccgct gattggtgtt
    22501 acccagagct actcctgttc gctggagcaa atctctgatt tccgccatga aatgcgttat
    22561 cagttctggc acgattttct cggcaacgcg ccgaccattc cgccagtgct ctacggcctg
    22621 aatgaaacgc gtccgagtca ggataaagac gacgaacaag aggtattcta taccaccgcg
    22681 ttagcccagg atcaggcaga tggctatgta ctgacggggc atccggtgat gctgcagggc
    22741 ggcgaatatg tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga gtttatcctg
    22801 acagtatacg gaacgtgcat gccaatgctc aacctgacgc gccgtaaagg tcaggatatt
    22861 gagcgatact acccggcaga agacgccaaa gcgggagatc gcccaattaa tctgcgctgt
    22921 gaactgctga ttcctatccg ccgttaacgc tgcagctcat ctaatgcagg ggcgtccaga
    22981 tgcgagatgt cccctgcggt ttcaacgacc cagccagacg ccagccacag gctttcctga
    23041 taatccacgc gcgaaataga acagttacgc agacgtaagc ggcgctctgc ccatgctggt
    23101 aatccgagaa tcgtactcac caggcatccc agtgcaatac catgactcac cagcagcggt
    23161 cggcttccct gcggtaagtc ccggcaagat tccagtgccg cattgacgcg atcgctgagc
    23221 tcctgcattg actcaccttc aggaatacgc ccgtcaacgg tgccattgac cagctgccga
    23281 cgccagttct cttcttcttc ggtcagagaa tcgatatgtc ttttttccag cacacccatg
    23341 tttaattcac gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg ggcgatgatt
    23401 tccgccgtac gccgggtgcg tcctaaatcg ctactgataa tatgcgtaat gccaagctct
    23461 ttggcacggg ttgccacctg catcgcctgt tgctcacctt tggcggtcag cgggctgtca
    23521 gactggccct gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg gactaggtat
    23581 acctgtaaca tgcttttttt ccgtatactg ctgtaacgtt aattttctga gtaatgctga
    23641 ttatgcacca agttgtctgt gcgaccacca atcccgctaa aattcaggcc attctgcagg
    23701 catttcacga gatcttcggc gaaggatcct gccatattgc atccgttgcc gtcgagagcg
    23761 gtgtaccgga acagcccttt ggcagtgagg aaacgcgcgc tggcgcacga aatcgggtag
    23821 caaatgcccg ccgtttactt ccagaggctg atttttgggt ggcgattgaa gctggcatcg
    23881 atggtgacag cactttcagt tgggtggtga ttgaaaacac cagccagcgc ggcgaggcgc
    23941 gttcggctac tttgccgtta cctgcggtga ttctgcagaa agtgcgtgaa ggtgaggcgc
    24001 ttggtcccgt gatgtcgcgt tataccggta ttgatgagat tggtcgtaag gaaggggcga
    24061 tcggcgtgtt taccgccggg aaactcactc gcgccagcgt ctatcaccag gcggtgatcc
    24121 tggcgctgag tccgtttcac aatgccgtgt atcaggcatt gtaggacgga taaggcgttc
    24181 acgccgcatc cggcattcgg tgcacgatgc ctgatgcgac gctgccgcgt cttatcagta
    24241 tctacaaaat caatcgcttt tcagcaacac ctcttccagc cactggcgca gctcaacggg
    24301 cgcggctttc aggctgttag atccacgcgt aatcgtcgcg atgcccgcgc cgagttcatt
    24361 ttttaactca cgctggctca tttcgccgcg caacagctct tcgacaatac gcacgcgagt
    24421 ccccaacgct tcgcgctcat ctggcgtcag catcaggttt aacaacggta aatggagatc
    24481 gttttggtag gcattcttaa gcaggtcgac aaaacgtaac cactcctggt gacgctgttc
    24541 tgccatcgct gctgaatagg gtgattgttg ggccataata tgtcgccatt gttagcgggg
    24601 gaagcaaaat gcctcccccg gttgtactcg ctaaagagta cgatagcata tcataaacgt
    24661 gcggatcagt aacgacgtcc ccattccgtg gcgctcatca acgtcggttt atcccccatg
    24721 aaatagcggt agtaagcgtc ataagccagc acgttcttca cataaccgcg cgtctcggag
    24781 aacggaatac tctcgacaaa tgccacagcg tcgatacgcc cggcgctgtt gccaagccag
    24841 gttcgcaccc gtccaggtcc ggcgttataa gctgctgagg agaaaatacg gttattgcca
    24901 aactgctgat aaacatattg caggtaactg gtgccaatgt tgatattcgt ttccggatcc
    24961 agcaattgcc cagggctgct ataaccagga atagagaaca tcttcaccgt atgggtcgct
    25021 gtaccaggca taatctgcat caggccgctg gcccctaccg gtgatttcac tttcggattc
    25081 caggcgctct cctgacgggc aatcgccatc gcatagcttt gcgggatctc tttcccactg
    25141 gtgtaacgtt tgaaaagatc gttgtaagcc agcgggaatc gctcttccag atgatcccaa
    25201 agcttcccgg cgatcgttgc ctgaacgcta agatcccacc attggttatt gaaagcatac
    25261 cgagccagtt gagcctgctc tgtttttgac ttgctcttca ccagattggc ccactcgcta
    25321 cgcgcggtgt tatcgagatt ccagtacatc aactcgcgca cgcgcgccat ctccggcccc
    25381 tgagtcaggg cgctgtcaac attctgcggc gctttatcaa tcttcagctc atactcttct
    25441 ccgatgcgtt gtgctgcaac catcgggtag aaaccacgct gttgcatgag ttgatgcaaa
    25501 atctctttcg cttcagcttc acgtccgcgt tccagcaata aatccgcctg ccagtaacgc
    25561 cattcatctt tctcttttgc ttccatcggc aaacgcgcca gccaggtatt caggccgcga
    25621 cgatcgccgg tgccaagtgc cattcgcaca cggcgttcaa tcaacgacgt ggattgcgag
    25681 cgcataatgg cgtcatcgcg ccatttcgcc tgctcgtcgg tgacatcgtt gcccatcaaa
    25741 cgccaggcga cgatatcgcg cagctcctga atctgatctt cattaagctg ctgcgcctgg
    25801 gcaagcgacg ggatcatcag ccgtgcattt tcagcatcct gccgcgccac actggcaaac
    25861 gccaccgctg ccatttgacg ggtaaaatcg gtcgcgccgg ttgtacgcgc gaaggtcagt
    25921 accgtattag ggttgttcgc cagtgaaatg attgccgagg cgatagtctg gtaatcggca
    25981 ggcatctgcc ctgccagcac tgttaccagg ccagtgttac ccgctttcat cgccagacgg
    26041 atacgctcta aatacgccag cggatcttgt ttacctgacg cacgccagac gctaaataac
    26101 ttgtcacagg cattaggctg gctcttgccg gttagccaca gctctttcgc cccttgccag
    26161 gcttcttcac tctgcccggt gttccatttc gcatagtagt aattacattg cgcttcggta
    26221 gttccgggct tttccgggct aaaggctaac aagccacgcc agtcttcacg ccgcgccagt
    26281 tcattgacga aacgagattg cagcgtgcga gcgggaggaa gcgtggggtt agcgcgaaca
    26341 aagttagtga ccgtcaccgc cggttgattc atcagatcat cggtgatctg gcggtattcc
    26401 aggtagggat aaagcggata atccttcagt ccaggcatca tttgttcgac cacatccatt
    26461 tgtcgattat cccaggcctg cttgatttgc gcgtaacggc tacgctgctc atccagtgag
    26521 tcggctcgcg ccacgctgct gaccgtcagc agacagacac cggcagccaa cagccgccag
    26581 gtaacttgtt tggctttttc cacaagcgca tcctctaagt gtaaatacat caatgcagcg
    26641 tcatgccgcg taatagattt atgctaacca gtcattgccg tttacgccac gttacggaca
    26701 cttttttact tttactgcga aggtgatcga ccaactggga ttaggcgggg aaaaaggcta
    26761 cacttcgcct ttgaaaactc atcatcacga taaacataga ggcgaagtcc aacgtggctc
    26821 aattcgttta taccatgcat cgtgtcggca aagttgttcc gccgaaacgt catattttga
    26881 aaaacatctc tctgagtttc ttccctgggg caaaaattgg tgtcctgggt ctgaacggcg
    26941 cgggtaagtc taccctgctg cgcattatgg cgggcattga taaagacatc gaaggtgaag
    27001 cgcgtccgca gccagacatc aagattggtt acctgccgca ggaaccgcag ctgaacccgg
    27061 aacacaccgt gcgtgagtcc attgaagaag cggtttctga agtggttaac gccctgaaac
    27121 gcctggatga agtgtatgcg ctgtacgccg atccggatgc cgattttgac aagctggccg
    27181 ctgaacaagg ccgtctggaa gagatcattc aggctcacga cggtcataac ctgaacgtac
    27241 agctggagcg tgcggcggat gcgctgcgtc tgccggactg ggacgcgaaa atcgctaacc
    27301 tctccggtgg tgagcgtcgt cgcgtggcgt tgtgccgcct gctgctggaa aaaccagaca
    27361 tgctgctgct cgacgaaccg accaaccacc tggatgccga atccgtggcc tggctggaac
    27421 gcttcctgca cgacttcgaa ggcaccgttg tggcgattac tcacgaccgt tacttcctcg
    27481 ataacgttgc aggctggatc ctcgaacttg accgcggtga aggtattccg tgggaaggca
    27541 actactcctc ctggctggag cagaaagatc agcgcctggc gcaggaagct tcacaagaag
    27601 cggcgcgtcg taagtcgatc gagaaagagc tggagtgggt acgtcaggga actaaaggcc
    27661 gccagtcgaa aggtaaagca cgtctggcac gctttgaaga gctgaacagc accgaatatc
    27721 agaaacgtaa cgaaaccaac gaactgttta ttccacctgg accgcgtctg ggcgataaag
    27781 tgctggaagt cagcaacctg cgtaaatcct acggcgatcg cctgctgatt gatgacctga
    27841 gcttctcgat cccgaaaggg gcgatcgtcg ggatcatcgg tccgaacggc gcgggtaaat
    27901 cgaccctgtt ccgtatgatc tccggtcagg aacagccgga cagcggcacc atcactttag
    27961 gtgaaacggt gaaactggcg tcggttgatc agttccgtga ctcaatggat aacagcaaaa
    28021 ccgtttggga agaagtttcc ggcgggctgg atattatgaa gatcggcaac accgagatgc
    28081 caagccgcgc ctacgttggc cgctttaact ttaaaggggt tgatcagggt aaacgcgttg
    28141 gtgaactttc cggtggtgag cgcggtcgtc tgcatctggc gaagctgctg caggttggcg
    28201 gcaacatgct gctgctcgac gaaccaacca acgacctgga tatcgaaacc ctgcgcgcgc
    28261 tggaaaacgc cctgctggag ttcccgggct gtgcgatggt tatctcgcac gaccgttggt
    28321 tcctcgaccg tatcgccacg cacatcctgg actaccagga tgaaggtaaa gttgagttct
    28381 tcgaaggtaa ctttactgag tacgaagagt acaagaaacg cacgctgggc gcagacgcac
    28441 tggagccgaa gcgtatcaag tacaagcgta ttgcgaagta atgcgtaaaa tgccggatgc
    28501 ggcgtgaacg ccttatccgg cctacaaacc aagaaatttc aatacgttgt gaatctccag
    28561 gcctgataag acgcggtaag cgtcgcatca ggcattgagc accgaatgcc ggatacggcg
    28621 tgaacgcctt ttccggccta caacagcctt agtatccgca aattcgtagc gggtcacccg
    28681 tgctgattct tttaaacctt gtttaatctc gacagcctct tccagagagg ttttcaactc
    28741 atcaaaaaac ttattttata cctcgccttt gagccgtttc atcgcggtta tctctgctcc
    28801 cccatcatct cccgcaccaa ctccacgcag cgcaggaaac ggctgtcata atcgtcctct
    28861 tcaacccgca cgaattcgat attgttctct tccagcatct ccaccagcaa gttctggaac
    28921 tctttgcgat ccaccgaact gccgaggctg cgtaaaccat ccgccaccca cggcgtgttg
    28981 ttctccagca ggatcaccag atcgaaacgg tattcatcaa tcaacgcctg tacgaacgga
    29041 tgctcacgcc cttcgtactt tttgcagaac gcctgggtag tgacaaaatc agtgtcaata
    29101 aacgccactt tattggcata tttcactgca aaatcaatgt attgtgcgtg gcccagcgcg
    29161 attttatcgt aatcggaata ctgcaatgcg atctcatcac cgccgaggtg tgaaaagaca
    29221 taatcgcgac catattccca cgcactggtg gtattgaaga tattggcaag tttgtttacc
    29281 agggtggatt taccgctcga ctcgccacca aggatcgcca cggtacgtac aaagaacggc
    29341 ttcacttccg taggaatata ttcccagtag cggaacgggt tttcgcggat ctgcgcaccg
    29401 ctgatactca taaaggtacg tttcggatcg accagcaccg tatcgatccc cagatgttcc
    29461 atatactgtg gcgcatcggc ttcttccgag gtgtagatca ggtccggctg aatccccttt
    29521 tcagccataa actttttgat gccgttgctc cacacatccc agccgtgcgg atacggctcc
    29581 atgccctctt cgttgaaagc atgaatgcga atatttttct gatatttaaa agtttgtaat
    29641 aaccagcgca gacgatccgg caccgtgggc tgctgcgaca tggcgctgtc ttcaaacaac
    29701 gcgcggtcgc gggtatcgtc aaaacccata atgatatgca gctcgtcaac ctggctacag
    29761 gcgcgctgga taaggtagat atgtccggta tgcagcgggt agaacttacc gaatacgaca
    29821 ccaatggttt tcttctgtcg gggaaattca agccccaaaa aacggtgcaa cgcctccagc
    29881 ttttgcgcgc tggggctttt aattttggca ttcagtaact ggcttaaata ccctttggtc
    29941 ataccgctgg catcagctac ctgctgtagc gtgcagccct gttgcttgat ggcagttttc
    30001 aggtaatcaa atgacgacat atctccctcc gtatctctca ttataagtcg tcgaacacgc
    30061 taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat cccttccggc gcttttttcg
    30121 gtacgttagc cgccggaaca atcgcccggc gaaaaccgtg tttcgccgct tctgaaattc
    30181 gttcctgacc gctgggcacc gggcggatct cccctgccag cccgacttca ccaaacacca
    30241 ccagatcctg tggcagcggt ctgtcacgca ggctggaaac catcgccagc agtaacgcta
    30301 aatcggcact ggtttcggtt accttcacgc cgccgaccac gttcacaaat acatcctgat
    30361 cggccatttg cagaccaccg tgacggtgca agacagccag caggattgcc agacggtttt
    30421 gttccagccc cactgccacg cggcgcggat tcgccatcat cgagtgatcg accagcgcct
    30481 gaatctccac cagcaacgga cgcgttcctt cccacaccac catcacggag ctgccggagg
    30541 tcacttcatc tccgcgactt aagaaaattg ccgaagggtt gctgacttca cgcagcccct
    30601 gttcggtcat cgcgaagacg cccagctcat tcaccgcgcc gaagcggttt ttatggctgc
    30661 gcaaggtgcg aaaacgggag tcggcatcac catccagaag caccgaacag tcgatacagt
    30721 gttccagcac tttagggcca gccagcgagc catctttggt tacgtgccca accatgacaa
    30781 tcgccacacc gcgcgttttg gcgaagcgcg tcagataagc cgccgtttca cgcacctgcg
    30841 ccacgctacc aggcgatgat tgtacatccg ccatatgcat cacctggatc gagtcaatta
    30901 ccatcagttt cggttgctct tcttcggcaa tcaggcagat ctgttcgatg ctggtttccg
    30961 acaacatatt gagattgtca gtcggcaggc caaggcgatg agcgcgcatt gccacctgtt
    31021 gcagcgactc ttcgccagtg acatacagcg ttttcatctg ctgggccagt ttgcacagcg
    31081 tttgcagtag cagcgtggat ttccccgcac ctgggttacc gccaatcaga atggcgcttc
    31141 ctggcaccac gccgccgcct agtacgcggt cgaactcttt aaatccggtg gaaaaacgcg
    31201 gcagctcttc aaggctgata tcggagagtt tctggacttt tgccaccccg gcgctaccgg
    31261 catagccgct gagacgctcg ttacgcgcca ccgttggcga cgcagcaaga cgcacctcgg
    31321 tgatggtgtt ccaggcatga caggcactgc actgcccctg ccagcgcgga taatcggccc
    31381 cgcattcatt acaaacaaag gcgcgttttg gagcttttgc cacggtgtac ctcgttaatg
    31441 ctgtgccgcc cgcaggatgg cgggcgagca attacttctg attcaggctg ccagagagga
    31501 tgcagaatac ccccatcagg tcagcgtgac ggatggtgac ttccgccttt tcattcactt
    31561 ttggcttggc atggtaggca atccccagcc ctgctgcttt gatcatcggc aggtcattag
    31621 ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc atactcctgc gcgaggcgag
    31681 tcaaagtttt cgctttgtac tgcgcgtcta cgatgtcgcc gatcacattg ccggtaaatt
    31741 taccgtccat gatctccagt tcattggcta ccacggcggt caggcgcagc ttgtcgcgca
    31801 gatattcagc aaagaaagta aagccgccgg aggcaatcgc cactttccag cccagcgttt
    31861 ccagcttgag caccagttgc gttaagcctg gcatcagcgg cagattttca cgcacctgtt
    31921 gcagaatatt ggcgtcagcg cctttcagcg tcgccacacg gctgcgcagg ctggcggtaa
    31981 aatcgagttc gccgcgcatc gcccgttcgg ttacttccgc caccatctcg cccgttccgg
    32041 ccagtttggc aatttcatca atacattcaa tctggatggc ggtggaatcc atgtccatca
    32101 ccagcaaacc cggcgtgcgc aggtgcggga ttttccccag cggggcgaca tccagttgcg
    32161 cttcatgggc caggcgcgta gcccgtgcgg tgagtgaacc tgccagacga atcacctgat
    32221 aatcttccac gcaccaggcg gcaacaatca ccatcgccgc acccagtttg ctctggtatt
    32281 gggtcagacg ctgtttatcc agcccacgac catacagcag ccagccgcta cgacctgcgt
    32341 ggtaatccag tggcatcact tcatcaccac ttaatgaaag aggcagacca ggccataaag
    32401 agacatcttc aggcaggtcg caccaggtaa tgttaggcat taaggctcct gtaaaatcgt
    32461 tcgaagcagg gaaaataacg catgaggcta ccttgtatcc attgcttctg gcaacattaa
    32521 gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa ctgaaattcc ggctgcatcg
    32581 ggcagtgatt gtcctgttct gtcttgcctt gttagtggcg ctgatgcagg gagcgtcatg
    32641 gtttagtcaa aaccaccagc gacaacgtaa tccacagctg gaagaactgg cccgcaccct
    32701 ggcgcgtcag gtaacgctga acgttgcacc gctgatgcgt accgactcac cggatgaaaa
    32761 acgcattcag gcgatcctcg atcagttaac ggatgaaagc cgtatcctcg acgcgggtgt
    32821 gtatgacgaa caaggcgatc ttatcgcacg ttctggcgaa agcgtcgaag tgcgcgaccg
    32881 gctggcgctc gacggtaaaa aagcgggcgg ctattttaac cagcagattg tcgaaccaat
    32941 tgccgggaaa aacggaccgc tcggctatct gcgcctgacg ctcgacaccc atacgctcgc
    33001 caccgaagcc caacaggtgg ataacaccac taacatttta cgcctgatgt tgctgctttc
    33061 actggcaatc ggcgtagtgc tgacccgcac gctgctacag ggtaaacgta cccgctggca
    33121 gcaatcgccc ttcctgttaa ccgccagcaa accggtgccg gaagaggaag aaagcgagaa
    33181 aaaagagtga cccattacta caagaaagga aatcgttatg tccacattac gcctgctcat
    33241 ctctgactct tacgacccgt ggtttaacct ggcggtggag gagtgtattt ttcgccagat
    33301 gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc gacacggtag tgattggtcg
    33361 cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg gaagaagata acgtccgcct
    33421 ggcacggcgc agtagcggtg gcggcgcggt gttccacgat ctcggcaata cctgctttac
    33481 ctttatggct ggcaagccgg agtacgataa aaccatctcc acgtcgattg tgctcaatgc
    33541 gctgaacgcg cttggcgtca gcgccgaagc gtccgggcgt aacgatctgg tggtgaaaac
    33601 cgccgaaggt gaccgcaaag tctcaggatc ggcctatcgc gaaaccaaag atcgtggctt
    33661 ccaccacggc accttgctgc tcaatgccga ccttagccgc ctggcaaact atctcaatcc
    33721 ggataaaaag aaactggcgg cgaaaggcat tacctcagtg cgttcccgcg tgaccaacct
    33781 caccgagctg ctgccgggga tcacccatga gcaggtttgc gaagccataa ccaaggcctt
    33841 tttcgcccat tatggcgagc gtgtagaagc ggaaatcatc tccccggaca aaacgccaga
    33901 cttgccaaac ttcgccgaaa cctttgcccg tcagagtagc tgggaatgga acttcggtca
    33961 ggctccggca ttctcgcatc tgctggatga acgctttagc tggggcggcg tggaactgca
    34021 tttcgacgtt gaaaaaggcc atatcacccg cgcccaggtg tttaccgaca gcctcaaccc
    34081 cgcgccgctg gaagccctcg ccgggcgact gcaaggctgc ctgtaccgcg cggatatgct
    34141 gcaacaagag tgcgaagcgc tgttggttga cttcccggac caggaaaaag agctacggga
    34201 gttgtcgacg tggatagcgg gggcggtaag gtaactcgct gattaaaata ccaaaggaag
    34261 ggaatatgat cgatctggaa aaccaggagc gggagattat caatttaatg ttaagccagc
    34321 gtatttcctg gctagccgcc gtgcgtatca ggcataaatt atcgcttgct gaagtaagta
    34381 aaatgttagg tatctcgatt aattctctaa aatagatcga aaagacggaa cggtttagct
    34441 caaacatcaa aagcaaaatg gcagaaattt atggttgtcc accagagttg ctgatatgcc
    34501 cttcctggat gacggctgaa c
//
LOCUS       DAZGTR010000040        33923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGTR010000040 DAZGTR010000000
VERSION     DAZGTR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.040"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            269..835
                     /gene="mntP"
                     /locus_tag="ACHLCY_002107"
     CDS             269..835
                     /gene="mntP"
                     /locus_tag="ACHLCY_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP0369364.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(832..1641)
                     /gene="rlmA"
                     /locus_tag="ACHLCY_002108"
     CDS             complement(832..1641)
                     /gene="rlmA"
                     /locus_tag="ACHLCY_002108"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP0369365.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1807..2016)
                     /gene="cspE"
                     /locus_tag="ACHLCY_002109"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1807..2016)
                     /gene="cspE"
                     /locus_tag="ACHLCY_002109"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0369366.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2029..2172)
                     /gene="yobF"
                     /locus_tag="ACHLCY_002110"
     CDS             complement(2029..2172)
                     /gene="yobF"
                     /locus_tag="ACHLCY_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP0369367.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2841..3128)
                     /gene="yebO"
                     /locus_tag="ACHLCY_002111"
     CDS             complement(2841..3128)
                     /gene="yebO"
                     /locus_tag="ACHLCY_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP0369368.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(3203..3346)
                     /gene="mgrB"
                     /locus_tag="ACHLCY_002112"
     CDS             complement(3203..3346)
                     /gene="mgrB"
                     /locus_tag="ACHLCY_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP0369369.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     NPW"
     gene            3505..3744
                     /gene="yobH"
                     /locus_tag="ACHLCY_002113"
     CDS             3505..3744
                     /gene="yobH"
                     /locus_tag="ACHLCY_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP0369370.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(3887..4678)
                     /gene="kdgR"
                     /locus_tag="ACHLCY_002114"
     CDS             complement(3887..4678)
                     /gene="kdgR"
                     /locus_tag="ACHLCY_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP0369371.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4855..6228
                     /gene="yebQ"
                     /locus_tag="ACHLCY_002115"
     CDS             4855..6228
                     /gene="yebQ"
                     /locus_tag="ACHLCY_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0369372.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASRKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(6274..7155)
                     /gene="htpX"
                     /locus_tag="ACHLCY_002116"
     CDS             complement(6274..7155)
                     /gene="htpX"
                     /locus_tag="ACHLCY_002116"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP0369373.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7347..9395)
                     /gene="prc"
                     /locus_tag="ACHLCY_002117"
     CDS             complement(7347..9395)
                     /gene="prc"
                     /locus_tag="ACHLCY_002117"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP0369374.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9415..10113)
                     /gene="proQ"
                     /locus_tag="ACHLCY_002118"
     CDS             complement(9415..10113)
                     /gene="proQ"
                     /locus_tag="ACHLCY_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP0369375.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10210..10707)
                     /gene="msrC"
                     /locus_tag="ACHLCY_002119"
     CDS             complement(10210..10707)
                     /gene="msrC"
                     /locus_tag="ACHLCY_002119"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP0369376.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10837..12120
                     /gene="yebS"
                     /locus_tag="ACHLCY_002120"
     CDS             10837..12120
                     /gene="yebS"
                     /locus_tag="ACHLCY_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP0369377.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12089..14722
                     /gene="yebT"
                     /locus_tag="ACHLCY_002121"
     CDS             12089..14722
                     /gene="yebT"
                     /locus_tag="ACHLCY_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP0369378.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            14802..16241
                     /gene="rsmF"
                     /locus_tag="ACHLCY_002122"
     CDS             14802..16241
                     /gene="rsmF"
                     /locus_tag="ACHLCY_002122"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP0369379.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLDD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQATAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     TGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPT
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            16359..16595
                     /gene="yebV"
                     /locus_tag="ACHLCY_002123"
     CDS             16359..16595
                     /gene="yebV"
                     /locus_tag="ACHLCY_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP0369380.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16700..16891
                     /gene="yebW"
                     /locus_tag="ACHLCY_002124"
     CDS             16700..16891
                     /gene="yebW"
                     /locus_tag="ACHLCY_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP0369381.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(16892..17548)
                     /gene="pphA"
                     /locus_tag="ACHLCY_002125"
     CDS             complement(16892..17548)
                     /gene="pphA"
                     /locus_tag="ACHLCY_002125"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0369382.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(17944..18285)
                     /gene="yebY"
                     /locus_tag="ACHLCY_002126"
     CDS             complement(17944..18285)
                     /gene="yebY"
                     /locus_tag="ACHLCY_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP0369383.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(18298..19170)
                     /gene="copD"
                     /locus_tag="ACHLCY_002127"
     CDS             complement(18298..19170)
                     /gene="copD"
                     /locus_tag="ACHLCY_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP0369384.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLICATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(19174..19548)
                     /gene="yobA"
                     /locus_tag="ACHLCY_002128"
     CDS             complement(19174..19548)
                     /gene="yobA"
                     /locus_tag="ACHLCY_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP0369385.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            19687..19917
                     /gene="holE"
                     /locus_tag="ACHLCY_002129"
     CDS             19687..19917
                     /gene="holE"
                     /locus_tag="ACHLCY_002129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP0369386.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            20019..20675
                     /gene="yobB"
                     /locus_tag="ACHLCY_002130"
     CDS             20019..20675
                     /gene="yobB"
                     /locus_tag="ACHLCY_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP0369387.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            20699..21361
                     /gene="exoX"
                     /locus_tag="ACHLCY_002131"
     CDS             20699..21361
                     /gene="exoX"
                     /locus_tag="ACHLCY_002131"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP0369388.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(21358..23418)
                     /gene="ptrB"
                     /locus_tag="ACHLCY_002132"
     CDS             complement(21358..23418)
                     /gene="ptrB"
                     /locus_tag="ACHLCY_002132"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP0369389.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSHLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(23627..24286)
                     /gene="yebE"
                     /locus_tag="ACHLCY_002133"
     CDS             complement(23627..24286)
                     /gene="yebE"
                     /locus_tag="ACHLCY_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0369390.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(24613..24969)
                     /gene="yebF"
                     /locus_tag="ACHLCY_002134"
     CDS             complement(24613..24969)
                     /gene="yebF"
                     /locus_tag="ACHLCY_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP0369391.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(25036..25326)
                     /gene="yebG"
                     /locus_tag="ACHLCY_002135"
     CDS             complement(25036..25326)
                     /gene="yebG"
                     /locus_tag="ACHLCY_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP0369392.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            25460..26638
                     /gene="purT"
                     /locus_tag="ACHLCY_002136"
     CDS             25460..26638
                     /gene="purT"
                     /locus_tag="ACHLCY_002136"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP0369393.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEQLQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGAGLQIRLFGKPAINGSRRLGVALATAESVVDAIKRAKHAAGQVKVQG
                     "
     gene            complement(26694..27335)
                     /gene="kdgA"
                     /locus_tag="ACHLCY_002137"
                     /gene_synonym="eda"
     CDS             complement(26694..27335)
                     /gene="kdgA"
                     /locus_tag="ACHLCY_002137"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0369394.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVHVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(27372..29183)
                     /gene="edd"
                     /locus_tag="ACHLCY_002138"
     CDS             complement(27372..29183)
                     /gene="edd"
                     /locus_tag="ACHLCY_002138"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP0369395.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTIHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(29418..30893)
                     /gene="zwf"
                     /locus_tag="ACHLCY_002139"
     CDS             complement(29418..30893)
                     /gene="zwf"
                     /locus_tag="ACHLCY_002139"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP0369396.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GVMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            31231..32100
                     /gene="hexR"
                     /locus_tag="ACHLCY_002140"
                     /gene_synonym="yebK"
     CDS             31231..32100
                     /gene="hexR"
                     /locus_tag="ACHLCY_002140"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP0369397.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            32228..33670
                     /gene="pyk"
                     /locus_tag="ACHLCY_002141"
     CDS             32228..33670
                     /gene="pyk"
                     /locus_tag="ACHLCY_002141"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP0369398.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(33730..>33923)
                     /locus_tag="ACHLCY_002142"
     CDS             complement(33730..>33923)
                     /locus_tag="ACHLCY_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HFP0369399.1"
                     /translation="TINDSIKTWVQTQYSKDSGLPMAVIGYSVAEEAGMRRLASYLDL
                     HSGYPCIHFTGGCDYDWIE"
BASE COUNT         8269 a         8664 c         8290 g         8700 t
ORIGIN      
        1 caacagttgt tttatattct caaaatatgt taaggttgcg ccctcatttg gggagtagcc
       61 gatttccaga ttccggaaat gtacgtgtca acatactcgt tgcaaaacgt ggcacgtacg
      121 gactgaatac tttcagtcag gcgagaccat atgcacatca atcgctatgc ctgcatgaac
      181 gcgaaaacca tcgcggcttg ttgttcatgt atgcatgttt gctgggggcg atgatgtgtt
      241 ttatggatac cccggtcagg acattgtcat gaatatcact gctactgttc ttcttgcgtt
      301 tggtatgtcg atggatgcat ttgctgcatc aatcggtaaa ggtgccaccc tccataaacc
      361 gaaattttct gaagcattgc gaaccggcct tatttttggt gccgtcgaaa ccctgacgcc
      421 gctgatcggc tggggaatgg gcatgttagc cagccggttt gtccttgaat ggaaccactg
      481 gattgcgttt gtgctgctga tattcctcgg cgggcgaatg attattgagg gttttcgtgg
      541 cgcagatgat gaagatgaag agccgcgccg tcggcacggt ttctggctac tggtaaccac
      601 cgcgattgcc accagcctgg atgccatggc tgtgggtgtt ggtcttgctt tcctgcaggt
      661 caacattatc gcgaccgcat tggccattgg ttgtgcaacc ttgattatgt cgacattagg
      721 gatgatggtt ggtcgcttta tcggctcaat tattgggaaa aaagcggaaa ttctcggcgg
      781 gctggtgctg atcggcatcg gcgtccagat cctctggacg cacttccacg gttaataaga
      841 acgctgccag aggtgaatat taaagtccgt ctggcagtcg aacacttctt ttgctgccag
      901 tgtttgccag acttctggct tcgcacgcca ggcaaacggc gtcatctgca ataatgcgac
      961 ggcttcatca ccgcgaagac gcatcggata acacaactca tcactctgct gtaatgtaaa
     1021 accttccagt tgttctgcat gaggtgcatg aagatgtact tcattgtaaa tcagcccctt
     1081 cagctccatc aaatgtcgcg gtcccggcgt ggcagtaatg acccagccgc cgggcttcac
     1141 tactcgtgct aattcttctg ctttacacgg cgcgtaaata cgtattatgg cgtccatact
     1201 ggtatcggaa aacggcaaac ggtggctgga agcgacacaa agagtgacct gcggatagcg
     1261 tttcgccgcc gcttttatcg ctaccttcga gacatccagg ccaaacgtgg tgatttcggg
     1321 caacacatcg gcaaatgcgt gtgtgtaata cccttcacca cagccaatat ccagcaccgc
     1381 cgtggccttt tcatcaagcc gttcccgcag ttgggcgaca attgcatcac gcagcggctg
     1441 ataatgtccg gcatctaaga atgcgcggcg tgcttgtatc atttccgcgc tgtcgcccgg
     1501 atcacgagac cgtttatgct gaacgggcag cagattgaca tacccttctt tcgccatatc
     1561 aaactgatgt cgctggggac agatatagct gtttttttca cgcgaaagag gctgatggca
     1621 aagtggacag gaaaacgaca tgacaactcc ggcaggatac taaaggccgc aagtgtaacg
     1681 cgaatcacgc ggcgggcaaa tatccgaacg ccaggagcga agataaagca tagtaaaaag
     1741 cctcgcattc gacgaggctt tatagcgaga ttgaagcgta ttcacacttc agatcagtgg
     1801 attcgatcag atagctgtta cgttaacagc tgccggacct ttctggccgt cctgaatttc
     1861 gaactcaacg ttctgacctt cagccagagt tttgaagcca ttaccctgga tagcggagaa
     1921 gtgtacgaac acatctttgc tgccatcagc cggagtaatg aagccaaaac ctttagactc
     1981 gttgaaccac ttaacctgac ctttaatctt tgccatttga aaaattcctt agattgtttt
     2041 cttcgcccgc aggcataaca tagataaaac tgacacatta ctgcatgagg caccaatata
     2101 aggctcggca gagaagcggt attcaacgtc aacgtgttta ctcaggactt ctttactgaa
     2161 aatgccacac ataaacagaa ctgtacctcg tttaacccga aatctgttat cacatacaac
     2221 gttaattatg gcaagccatt tttaaacatg tctcgatcag acgcacaaat ctcgatacgt
     2281 cttccacttt ttttgcacac ttatgcaacg gaatacgcgc cgatatatca ttgtgcttaa
     2341 ccttgccagt tcaggcagat acttaaactg gcgtattttc taacatagtt caataagttc
     2401 agttgttcca ggccagtggg agaagttatt acatagtgcg tgcaatatca cattttttgc
     2461 tatgcaatga ataaaaagtt atatcacttt ttctcataaa acagtcagtt aacggctatc
     2521 aattacccta aagagaagtc aatccccaaa gggattgaaa atttaaaata agaaaaattg
     2581 atgaatgagc aaaaaatcaa gagagaaacg tttcgctaat aaataatacc acgcttaatc
     2641 acaaatccga tgtaaacatc ctaataaatt aatgaggaaa atatcttttt ctgatatccc
     2701 gttacgcaaa taaataatat gataattatt tgaagtcaga ccagagtttt aatttgaagc
     2761 agttgcacca tggcagtgcg tataatcagg atgaattgag taacccttca acgacggggt
     2821 tactcaagat tgaggaagga ttatcgttcg gcgaccagac gaataatatc ttcgtcttca
     2881 accgattttt gactctcaac ggtcttttta tctgctttct caggttcgtt tgcttcgcac
     2941 aaacgacgta acagtgcatt ttgacgtttt tgctgatcca gcaacgcctc aagcagttca
     3001 atctgttcgt tagtccgaga actggcacga ttgataaaaa accacaagat gagcccgata
     3061 agaagaacca ccaccgatac aaccaaagac gcaatgttca tcacgcctga atttacaact
     3121 tcgttcattt caccacctct gagtagaggc gctattctac cactgctgga gaggaagaaa
     3181 atctagtgct gaaaaaatga tatcaccacg ggttaaactg gttaatggca caaattccgc
     3241 tgaaaaattg tacatcctga tcgcacatca tgttgaatac ctgcgcccaa agcagcaagc
     3301 aagccaacac cacgacaacc agaacgaccc atcgaaactt tttcactcca atctccgctt
     3361 cacctacctt atgtcattaa actagcatgc gttagttaaa cagcgcctaa ctatgtcgat
     3421 attcgtgctt tttcggaatg agtagcttgc ttcagcgcat attgccgcta cgattaagcg
     3481 aacataaaaa gagaagaggt tgtaatgcga ttcatcattc gcacagttat gctgatagca
     3541 ctggtatgga taggattatt actcagcgga tatggcgttt tgattggaag caaagagaat
     3601 gccgcaggat taggtttgca atgtacctat ctgactgcca gaggcaccag cacggtacaa
     3661 tacttgcaca ctaaaagtgg tttcttgggg ataacagatt gcccactgtt acgtaaaagc
     3721 aatatcgtgg tcgataatgg ttaagggtaa taaataacca gattctcact catgagtaaa
     3781 atattcttat agcaagcgcg tttaatataa cgcaccttca actttatttt tctcatgcta
     3841 aataaagaac ctgtctgttt gatattccga caggttctta ctctcatcag aacggatagt
     3901 cgtgataacc catttgggca gaaattttgc gcgctgcggt atgcaacatt gcgacatatt
     3961 cctgtaaacg ctcttcagag aaacgcaacg tcgggaagga gatgctcaaa ccggcaatga
     4021 ccacgccaaa gcgatcaaat accggtaccg caatgcatcg cagcccttct tcctgctctt
     4081 cgttatcttc gccatacccc tgctcgcgca cctggtccag aacgggtaat aacgcttctg
     4141 tactggtgat ggtccgctcg gtactgcgtt tatactccac gccctcaaga atttgcttca
     4201 cttcatcgag atcgcgccat gccagcagta ccttaccaat cgcggtgctg tacagcggat
     4261 tacgacgccc aatccgtgaa tacatgcgca aattgtacat agagtcaatt ttgtgaatgt
     4321 aaacaatact gtcttcgtcc agtgcgccga ggtggatagt ttctttggtc aggcgggaga
     4381 tctcacgcat ctggatatct gcgctacgaa ttaaatcgac gttttgtaac gcgcgagcgc
     4441 ccagttcaaa caatttcagg gtcagggaat atttctccga ctccccttcc tgcgccacat
     4501 aacctaaggt tttcatggtc tgtaaaaagc gataaacggt gctttttgac atcatgacgc
     4561 gctgcgacag ctcggttatc cctatttcgc gctcttcacc cagcgcctgc aaaatgccaa
     4621 aaacttttag cacggaagat acagaatcag gctgtttatc cagatctgcg ttagccattt
     4681 atcacctcat tgcgagtgtt ttataaaaat cagaactgtt ttttattata atttcgcacc
     4741 agggtggtcg caatccatct tttgccggtt agttacaatt ctgcgacatc caccgtgaat
     4801 atcagtgcta gaatcatacc cctgttgatt attcaccaaa gatataaaat tcctatgcca
     4861 aaagttcagg ccgacggcct gccattgccc cagcgatacg gtgcgatatt aaccattgtg
     4921 attggcattt cgatggctgt ccttgacggc gcaatcgcca acgtcgccct gccaacaatc
     4981 gccacggacc ttcatgccac gccagccagt tccatctggg tagtgaacgc ctatcaaatc
     5041 gccattgtca tctccctgct ctcgttttcg tttctgggcg atatgtttgg ctatcgacgt
     5101 atttataaat gcggtctggt cgtttttctg ttgtcttcac tgttctgcgc cctttctgat
     5161 tcgctgcaaa tgctcaccct tgcgcgtgtc atacaaggtt tcggcggtgc agcgttgatg
     5221 agcgttaata ccgcacttat ccgcctgatc tatccacaac gttttctggg gagagggatg
     5281 ggcataaact cgtttattgt tgccgtttct tctgctgccg ggccgacaat tgctgcagca
     5341 atcctctcca tcgcatccag gaaatggtta tttttaatca acgtaccgtt aggtattatc
     5401 gccctgcttc tggcgatgcg ttttctgcca cccaatggtt ctcgcgccag taaaccccgt
     5461 ttcgacctgc ccagcgccgt gatgaacgcg ttaaccttcg gcctgcttat cactgcgttg
     5521 agtggtttcg ctcaggggca atcgctgacg ttaattgctg cggaactggt ggtaatggtt
     5581 gttgttggta ttttctttat tcgccgccag ctttctcttc ccgtaccgct gctaccggtg
     5641 gatttactgc gtatcccgct gttttcactt tctatttgca catctgtttg ctctttctgc
     5701 gcacaaatgc tggcaatggt ttccctgccc ttttacctgc aaaccgtgct cgggcgtagt
     5761 gaagtcgaaa caggtttact tctgacaccg tggccgttag caacgatggt gatggctccg
     5821 ctggcaggct atttgattga acgcgtacat gcaggattgc tgggtgcttt agggttgttc
     5881 atcatggctg cggggctttt ttccctggtt ctgctgcccg cgtcacctgc ggatatcaat
     5941 attatctggc cgatgatctt atgtggcgct ggatttggct tattccagtc acccaataac
     6001 cacaccatta ttacctccgc tccgcgcgaa cgtagcggtg gggccagtgg catgttagga
     6061 acggctcgtc tactgggtca gagtagtggc gcggcgctgg tggcgctgat gctaaatcag
     6121 ttcggtgata atggtacgca cgtttcgctg atggctgcgg ctattctggc agtgattgct
     6181 gcctgtgtga gtggtttacg tatcactcag ccacgatcca gggcataaaa aaagcgcgtc
     6241 gatcaggacg cgccttttag tatttacttc atattacttc aggtattcac ccgtacgcag
     6301 agcttcaatt cgtttatcca gcggcgggtg agtcatgaac aactcactga gcgatttcga
     6361 cttaccgtta atgcagaaag ccatcatgct ggttgcttct tgcggttcat agctggtttt
     6421 caggcgctgc agcgcggcaa tcattttctc gcgaccaacc agttttgccg aaccggcatc
     6481 agcatggaat tcacgatgac gcgagaacca catggtgata atgctcgcca gaataccaaa
     6541 caccagttcc agaaccgttg caaccgcaaa gtagatcagc gggttgccgt tgctctcttc
     6601 accttcatca cgatttccgc ccataaaacc cgcggcaagc tgcgccagaa tacgggaaat
     6661 aaagataacg aaggtgttca ccacgccctg aatcagcgtc atggtaacca tatcaccatt
     6721 ggcgatgtga ctgatttcgt gagcaattac cgcctcggct tcatccgggc tcatgttctg
     6781 cagcaaaccg gtgctgacag caaccagaga ggcatcacgg cgcgcaccgg ttgcaaaagc
     6841 gttgatgtcc ggcgcatggt agatagccac ttgcggcata gcgatccctg cctgacgagc
     6901 ctgggttgct acagtattga ccagccaacg ttccctttcg ttacgcggtt gctcgatcac
     6961 ttccccgcca acagatcgta atgccatcca tttggacatc agaagcgaaa cgaaggaacc
     7021 accaaaaccg aacagcaagg ccatgatcat cagcccctga acgctgctcg actgtatccc
     7081 tgtcaggctc agtaccagcc cgaaaacgac cattacggcc aggttcgtta gcaggaagag
     7141 cgcgattcgc atcataattt tctttttacc tcagtttaac aaaacgcaat atgcgatacc
     7201 cacatcgtat gggttacgcg actattttca agtctggata gtgcgtaagt caccagaaag
     7261 acacaacttt acattttgta gcatctgatt tacggcatct tgccgctgtt aaaaaatcag
     7321 gcacaatttc ttgtgcctga ttgatattac ttgacgggag cgggttgttc cgcgggtctg
     7381 gctttttcaa gcttcgccag atcgagtgcg atattcaccg tctcatccag ataaggatcc
     7441 ggctcctggt aatctttcgg tagatcatcc agcttcttca actccggttt accttcgcgt
     7501 ttaaagcgtt cgttcaaacg cgccagacgc gtcgcatcat cttcattatt ctctttctca
     7561 cgcacagcgt agttcagaga aacgatattg cgcttgtcct tcatagcgtt gaagcgcgcg
     7621 atatccttca taatgttctg gaactcagga tctttcgcga tacgcgcatt atgttccttc
     7681 agcagctccg gcccaaaggc cgttaaatcg cctgatttca cataagtcgc ggcatcaatg
     7741 ctatcccacg gcagcgcgtt atcttcgaat ttctcacccg tttccgtttc ttcattaccc
     7801 gtcggcatga tgatgtctgg cgttacgcct ttacgttgcg tactgccgcc gttaacgcga
     7861 tagaatttct ggatcgtgta ctgcacagaa cccagcgctg gccattcagg acgtaacatc
     7921 tgatcgtaaa tacggttcaa tgaacggtat tgctgaacgg tgcctttacc aaacgtcggt
     7981 tcacccacaa ccagcgcacg accgtaatcc tgcattgccg cggcaaagat ttctgaagcc
     8041 gaagcactga aacggtcaac cagtaccacc agcgggcctt tatagaacac ctgcccgtcg
     8101 gtatcgctgt cttcacgaac cttgccgttg ttatcgcgga cctgaacaat gggacccgca
     8161 ggaataaaca gacctgagag cgataccgct tcggtcaacg ccccaccgcc attgctacgc
     8221 aggtcgatga tcacactgct gacattctgt ttttccagtt tctgcagttg aactttgaca
     8281 tcgtctgtca aacccacata gaagcccgga atatccagca cgccgacttt ctctttaccg
     8341 acggtcttca ccgacatttt aaccgcgcgg tcttcgagac gaatacgttc acgggtcaat
     8401 gttacagtgc gggtcttggt ccctttacca gcaggtaaaa tttccagacg aactttactg
     8461 cccttcggcc ctttaattaa ggcaaccaca tcatcaagac gccagccaat cacgtcaacc
     8521 atcggcttgc ctgtttgacc aacaccgaca attttgtcac caacgctgat agctttactc
     8581 ttcgctgccg gaccacctgc caccatcgaa ttgataacgg tgtagtcatc atccatttgc
     8641 agcactgcgc caataccttc cagcgacaaa ctcatttcag tgttgaactg ttcggtatta
     8701 cgcggggaaa gatagttggt atgcgggtcg atttcacgcg caaacgccgt cattgccagc
     8761 gagaaaacat cttcgctgtt ggtttgcgcc agacgacgaa tggcaaattt gtagcggcgt
     8821 gtcagggttt cacgaatttc tttatccgtt tttcctgcca gcttcaggct taactcgtcg
     8881 aatttgactt tactgtccca cagcgcgttc aactcagcct cgtttttcgg ccagggcgct
     8941 ttgctgcggt caaggttata agtgtcgttg ccggtgaaat ccatcggctt ttccagtacc
     9001 gacaaagcgt actggtaacg ctcaaaacgg cgcttttgcg ccagattgta gagatcgtag
     9061 aaaacgtcga gtttgcctga acgcagttca tcgcctaact cggttttctt tttcgcgaac
     9121 tgttcaacat cgcttgccag cagcacgttg tggctgtaat cgagcagatt caggtagcgg
     9181 tcaaagattt tggccgaaaa tgcctgatcg aggtcgaact ggcgataatg agaacgggtg
     9241 aagcgcgacg ttacgcgctc acttaccgtc gcatgctgcg tctcttcctt taataccgga
     9301 atttgatcag cacgcgtgat atcttctaca gcgaaggtct ggcctgctat tgcaagcagg
     9361 ccagctaacg cggtaagcct aaaaaacatg ttcatgcctg gcccggcctc cgtttcagaa
     9421 caccaggtgt tctgcgcgca caatcaaaga catacccgaa ttcagctgga cgcggacgcc
     9481 gtctttggtg atttctaata cggtggcatc catcgcgttt tgacccgctt tcaccttcag
     9541 ggcttgtccg acagtcagag ctgaaatgtc agaaactggg gtgtgctgtt cttcgcgagg
     9601 tgcttttact gtttttggcg ctttctctac cggcttttgc gcacgaggtt tacgttcagc
     9661 gccttctttg cggcgtggcg tggtcggacg tggcttgcgt tcgcggcgcg gtgcgtcttc
     9721 tttctcacca gcagctgcgg cagcttcgcg ttttttcgct tgctgttcag cacgctgtgc
     9781 ctgaacacgc gctttcgctt cttcaagctg cttgcgagca tgctctacat gttgctcgtc
     9841 cagctcaccg catgggttgc cgtcaagatc gacacgcgtt gcgccgggtt taacaccgta
     9901 aagataacgc cagctcgaag tgtagagacg taaagcggat cgtaattgcg ttttgctcag
     9961 gttcatttcc ccggcaacac gatcgaccaa atcctgaaaa ataccgattt tcagcggacg
    10021 cgcttcacct tccgcactga aacagtgggg aaaacgttcg gccagaaacg cgattacttc
    10081 tttactgcta ttcaacttag gttgattttc catgaaattt cctgattaca acggacgtag
    10141 ccaacaagcg caggcatgaa caggcgtcat tataatgacg ctaccagtaa atgctacgtt
    10201 atccgttgat tatcctgcga cgctcgcaaa gaattttttg taatccgtcg ttgcaagcac
    10261 tttttcaagc tgtgccacaa gctgacgtaa gccttgctcg tcctcgtctg taaagcgacc
    10321 gaagacggta ctatcgatgt caagaacacc aataatctga tttttcacca ccagcggcag
    10381 aacaatttca gaattactcg ccgcatcaca ggcaatatgc ccgtcaaacg cgtgaacatc
    10441 ctcgatacgc tgcacttgat tgcgggcaac cgcagtgccg cacaccccgc gcccgacggg
    10501 tatccggaca caggcaattt tgccctgaaa tggtcctagt accagtgtat cgtcctcaag
    10561 caaataaaaa cctgcccagt ttacgtcagt gagacgctcg tataacaacg cactggtgtt
    10621 cgcaagcgtt gccagaaaac tggtttctcc cgccatcagc gcgttaaagt cgcgatttaa
    10681 atccgcgtaa aattctgttt tgttcattat ataatcactt ggttgtctta cctggaactg
    10741 ccagcctatt aaaataagca ttaaatgcgt taatgctcaa gatcattcca atcatgggtt
    10801 aatattaatg ttaattctta ttacatttgg cacgtcatgg ctcttaacac accacaaatt
    10861 acgccgacaa aaaagataac agtgagggca atcggcgagg aactgccgcg tggtgattac
    10921 caacgttgcc cgcaatgtga catgctgttt agcctgcccg agataaattc tcatcagagt
    10981 gcctattgtc cgcgctgtca ggcaaaaatt cgcgacgggc gcgactggtc gctaacgcgc
    11041 ctggcggcaa tggctttcac catgctgttg ctgatgccgt ttgcctgggg cgaaccgctg
    11101 ttgcatatct ggctgttagg tattcgtatc gacgccaacg tcatgcaagg catctggcaa
    11161 atgaccaaac agggcgatac gataacgggg gcgatggtct ttttctgcgt tattggtgcc
    11221 cccctcattc tggtgtcctc catagcatat ttatggtttg gtaaccgact gggaatgaat
    11281 ttacgtccgg tactgctgat gcttgagcgt cttaaagagt gggtaatgct ggatatctac
    11341 ctggtcggca ttggcgttgc ttctataaag gtacaggatt atgcccatat ccaggctggt
    11401 gtcggcttgt tctcttttgt ggcgttggtg attttaacga cggtgacgtt gtcacatctt
    11461 aatgttgagg agctgtggga gcgatattat ccgcagcgcc ccgctacgcg tagggacgag
    11521 aaacttcgtg tctgtcttgg gtgccatttt accggctatc ctgatcagcg tggtcgctgc
    11581 ccgcgttgcc atatcccgct acgcctgcgt cgccgtcata gtctgcaaaa atgctgggcg
    11641 gcgctgttag cgtcaatcgt tttgttgtta ccagccaacc tattgcctat ttctatcatt
    11701 tatctgaacg gaggacggca ggaagataca attctttccg gaattatgtc gctggcaagt
    11761 agcaacattg cggttgcggg aatcgtgttt atcgccagta ttctggtacc gtttactaaa
    11821 gtgatcgtca tgttcacttt actgttgagc attcatttta aatgccagca aggtttacgc
    11881 acacgcattc tgttactgcg gatggtgacc tggattggac gctggtcgat gctcgacctg
    11941 tttgtcatat ctttaaccat gtcgctgatt aatcgcgatc agatcctcgc ttttactatg
    12001 ggaccggctg cgttttattt tggcgcagcc gtaattttga ctattcttgc tgtggaatgg
    12061 ctggacagcc gcttactttg ggatgcacat gagtcaggaa acgcccgctt cgacgactga
    12121 agcgcagatt aaaaataaac gccgtatctc acctttctgg ctgctgcctt tcatcgcgct
    12181 aatgattgcc ggttggctga tttgggacag ttatcaggac cggggtaata ccgtcaccat
    12241 cgactttatg tcggcggatg gtattgttcc gggccgtacg cctgttcgtt atcagggcgt
    12301 tgaagtcgga acagtgcagg atatcagcct cagcgacgat cttcgtaaga ttgaagtcaa
    12361 ggtcagcatc aagtccgata tgaaagatgc gctgcgcgaa gagactcaat tctggctggt
    12421 gacgccaaaa gcatcgttgg caggtgtgtc cgggctggac gccctcgtcg gtgggaacta
    12481 tatcggcatg atgccgggta aaggtaaaga gcaggatcac tttgtcgcac tcgataccca
    12541 accgaagtat cggctggata acggcgatct gatgatccac ctgcaagctc ccgatctcgg
    12601 ttcgctgaac agcggttcat tggtctattt ccgcaagatc ccggtgggaa aagtctacga
    12661 ctatgccatc aatcccaaca agcaaggcgt ggtgattgat gtcctgatcg agcggcgttt
    12721 taccgatctg gtgaaaaaag gtagccgttt ctggaacgtt tccggcgttg atgccaacgt
    12781 cagtgtcagt ggcgcgaagg tgaaactgga aagtctggcg gcactggtta acggtgcgat
    12841 tgccttcgat tcacctgaag agtcgaaacc tgccgaggcg gaagatacct ttggtctgta
    12901 tgaagatctg gcccacagcc agcgtggcgt aataataaaa ctggaactgc cgagtggggc
    12961 cggattgacc gccgactcga cgccgttaat gtatcagggg ctggaagtcg gacagctaac
    13021 taaactggat ttaaatcctg gtggtaaagt cactggggaa atgacagttg atcccagcgt
    13081 cgttaccctg cttcgtgaaa atacccgcat cgaattacgt aatccgaaat tatcccttag
    13141 cgatgccaat ctcagcgccc tgctgactgg caaaaccttc gagttggtac ccggcgatgg
    13201 cgagccacgc aaagagttcg ttgttgtgcc aggcgaaaaa gcactgctgc atgaacctga
    13261 tgttctgacg ctgaccctga ccgcgccgga aagttacggt attgatgctg gtcagccgct
    13321 cattcttcac ggcgtgcagg taggccaggt tatcgatcgt aaactcacca gcaaaggcgt
    13381 cacctttacc gtcgccatcg agcctcagca tcgagaactg gtaaaaggcg atagcaaatt
    13441 tgtcgtcaac agccgtgtcg acgtgaaggt ggggctggat ggcgttgagt ttctcggtgc
    13501 cagcgcctca gaatggatta acggcgggat acgtattctg ccgggcgata aaggcgagat
    13561 gaaagccagc tatccactgt atgccaatct ggaaaaagcg ctggagaaca gccttagcga
    13621 tttacccacc acaaccgtga gtttgagtgc agagacgctg ccggatgtgc aggcaggatc
    13681 ggtagtgctc taccgtaaat ttgaagttgg tgaagttatt accgtccgtc cgcgagctaa
    13741 cgcgtttgat atcgatctgc atattaagcc ggagtatcgc aaccttctga ccagcaatag
    13801 cgtgttctgg gcagaaggcg gggcgaaagt tcagctgaat ggtagtggcc tgaccgtaca
    13861 ggcatccccg ctctccagag cattaaaggg agccattagc ttcgataacc tcagcggtgc
    13921 cagcgccagt cagcgtaaag gcgacaaacg aattctgtat gcttccgaaa cagcggcccg
    13981 tgcggttggt gggcagatta cgcttcacgc tttcgatgcc ggaaaactgg cggtcgggat
    14041 gccaattcgc tatctcggta ttgatatcgg gcaaatccag acgctggatc tgattaccgc
    14101 gcgcaatgaa gtacaggcaa aggcggtgct ctatccggag tatgtccaga ccttcgcccg
    14161 cggcggtacg cgcttctcgg tggtcacacc gcaaatttca gccgcgggcg ttgagcatct
    14221 agatactatc ctccagccgt atatcaacgt cgaaccaggc cggggcaatc ctcgccgcga
    14281 ctttgaatta caagaggcca ccattactga ttcgcgttac ctggatggct taagcattat
    14341 tgttgaagcg ccggaagccg gttcgttagg tattggtacg cctgtgctgt tccgtggtct
    14401 ggaagtcggt acggttacag gaatgacgct ggggacattg tcagatcgcg tgatgattgc
    14461 gatgcgcatc agtaaacgct atcaacacct ggtgcgtaac aattccgtct tctggttggc
    14521 atcgggttac agtctggact ttggtctgac gggcggcgta gtgaaaaccg gcacctttaa
    14581 ccagtttatc cgtggcggca tcgccttcgc cacgcctccg ggtacgccac tggcaccgaa
    14641 agcccaggaa ggcaaacact tcctgttgca ggaaagtgaa ccgaaagagt ggcgtgaatg
    14701 gggaactgcg cttcccaaat aatgcccact gctccggcgt gcctgcgccg gagcgtttat
    14761 gctaaactgc gcgcctgttt ttttgccagt ggtacatgct cgtggcccaa cacaccgttt
    14821 atttcccgga cgcctttctg acacaaatgc gcgaagcgat gccttcgacg ctctcatttg
    14881 atgattttct tgccgcctgt cagcgcccgt tgcgccgcag cattcgcgtt aatacgctga
    14941 aaatctccgt tgctgatttc ctgcaattaa ccgctcctta tggctggacg cttacgccaa
    15001 ttccgtggtg tgaagaaggt ttctggattg aacgcgacaa tgaagatgca ttgccattgg
    15061 gtagtaccgc cgagcattta agcggcctgt tttatattca ggaagccagt tcaatgttgc
    15121 ccgttgccgc cttgtttgct gacgataatg caccacagcg ggtgatggat gtcgcagctg
    15181 cgccaggctc caaaacgacg caaattgccg cgcggatgaa taacgaaggg gcaatccttg
    15241 ccaatgagtt ttccgccagt cgggtaaaag tgttacatgc caatatcagc cgctgtggca
    15301 tcagtaatgt tgcgctcaca cattttgatg gccgcgtgtt tggtgcggca gtgccagaaa
    15361 tgttcgatgc cattttgctg gacgctccct gctctggcga aggcgtggtg cgtaaagatc
    15421 ccgatgcgct aaaaaactgg tcaccagaaa gcaatcagga aatcgcagct acacaacggg
    15481 agcttatcga cagcgccttt catgcattac gtcctggtgg tacgctggtt tactcgacct
    15541 gtaccttaaa ccaggaagaa aacgaagccg tttgcctgtg gctgaaagag acttaccccg
    15601 acgcagtaga gtttttacca cttgacgatc tcttccctgg tgcaaacaaa gcgctgaccg
    15661 aagaaggctt tttgcatgtt ttcccacaaa tttacgactg cgaaggcttc ttcgttgctc
    15721 gtctgcgtaa aactcaggcg attcccgcct tacccgcccc caaatacaaa gtcggtaatt
    15781 ttccgttcag cccggtgaaa gatcgcgaag ctggacaaat tcgtcaggcg actgcaggtg
    15841 ttggcttaaa ctgggatgaa aacctgcgcc tctggcagcg tgacaaagaa ctgtggttgt
    15901 tcccggtggg cattgaagcc ctgatcggta aagtccgatt ttctcggttg gggattaaac
    15961 ttgccgagac gcacaacaca ggttatcgct ggcagcatga agcggttatt gctcttgcct
    16021 cacccgataa tatgaatgca ttcgagctga caccgcagga agcggaggag tggtatcgcg
    16081 ggcgcgatgt ttacccgcaa gccgcgccag tggcggatga tgtattggtt actttccagc
    16141 atcaaccgac tggtttagcc aaacggattg gttcgcgatt gaaaaacagc tatccgcgtg
    16201 aactggtgcg cgatgggaaa ctttttaccg gtaacgcctg acagctcaca aaaaaagcgc
    16261 actttttgac tggcacattc ggctgcctca actaggctga aaaatggtgc gatcggactg
    16321 gtcgtaccac aatcggcagc taaatggaga gcacgaagat gaaaaccagt gtgcgcatag
    16381 gcgcttttga aatcgacgac ggcgaattac acggtgaatc gccgggtgat cgaacgttaa
    16441 ccattccttg taaatctgac cccgatttat gtatgcaact cgatgcctgg gatgctgaaa
    16501 ccagtatccc tgccctgcta aatggcgaac actctgtcct ttaccgtacc cgttacgatc
    16561 aacaatctga tgcctggatt atgcgtcttg cctgatccaa aaagaacccg tcggcatggc
    16621 gggttatttg tcctggttat tcccccgttg taaaatctct cctaaactta acggtacggc
    16681 accacacttc ggggatgaaa tgttcgcgct ggtacttttt gtttgctacc tggatggcgg
    16741 ttgtgaagat attgttgtgg atgtctacaa cacggaacag cagtgtcttt attctatgag
    16801 cgatcaacgg atccgccatg gcggttgttt tccgattgag gattttatag atggtttctg
    16861 gcgaccagca caggagtatg gtgattttta attattgcaa ttgcacaaga gtcagttcgc
    16921 ccccaaagac agcaccggta tcaatataat gcaggttgcc aatatccacg cgatgtcgca
    16981 acggtgtatg accaaaccag aaatgatcag cacctgtaat tccctgccct ttttggcgtt
    17041 cacctaatcg cgagcggctc cacaagacct gatgcaaatc aacgtccttt tgccattcat
    17101 aaacatcatc tggataatcg gcatgagcaa taacatgttt gccggtacga ctgtgtactt
    17161 caagaataaa gggcaaatgc tgacattttt ccagcgccgt tttcgcttgt ttctgttgat
    17221 tatctgccag cgcaataaac cagtcgccgc cattcatcaa ccacaaagac atctgctggg
    17281 atgccagcgc atccatcgcc atctgttcat gattgcctct taccgcacaa acccaatgtt
    17341 gttccagtaa ctgcagacaa cgtaaacttt gcggcccacg atcgataacg tctcctactg
    17401 agataagtaa atctcgccac ggatcaaaac gacaatgcca taatttgcgg cgcaactgct
    17461 caagacaacc gtgtatatcg ccagaaagcc agatatgtcg ccattgatga cccgcaattc
    17521 tctgataaac gggcgcaggc tgtttcatca atattttcct cccgcgctaa agatcacata
    17581 atcttaacaa gaatgttaaa aaacgctgga ctcagacagt agagtgtgtg ttatggttga
    17641 ctataaagtc agcgaaggaa atgcttctgg cttttaacag ataaaaagag accgaacacg
    17701 attcctgtat tcggtccagg gaaatggctc ttgggagaga gccgtgcgct aaaagttggc
    17761 attaatgcag gcttagttgc cttgcccttt aagaatagat gacgacgcca ggttttccag
    17821 tttgcgtgca aaatggtcaa taaaaagcgt ggtggtcatc agctgaaatg ttaaaaaccg
    17881 cccgttctgg tgaaagaact gaggcggttt ttttattgga aatcaaaagg ctattttagg
    17941 taattaacag agtttttcag ctcgttctat aaacggtgcc agactcattt tttcgccggg
    18001 attgttagga tcatcaatct gaatcaccga aatgggttgg gctttagtct tcccactggc
    18061 aacttccttt tgtgcgatat cgtttaaagg atactgcacg agggtacttg ggttaatgac
    18121 atacaaagca ttacccggtc tgcaagtcag catcacctct tcgcgattaa acgcccattt
    18181 gtctttaccc acttcaaaac gactgacggt aatcacctgc ggtgcagcca gcgccgctgc
    18241 agaactggtg agtaacagaa acgtcagaat actttttttc atcattttcg taatccatca
    18301 aaaaggttcg agagtcgcaa gcaggctgat gatcaccagc actaccgcac ctatcgccca
    18361 ttcgagttta gtcatccact caaaccaggg tgccgcacga tcttcatctt gcctcatgcg
    18421 cggtacaaga acataacgat tagccagcgc aatgaccacc ataatcatca ccaggatggc
    18481 tttgagcaat aagagttggc cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa
    18541 caatgcatta agcacgccgc ttgccagtac gccgatcacc gcaaaatgcc cgcaccagga
    18601 aaaacgcatc agcgcctgaa tggcctggtg tcgccaacgg cctttgatga gttgcataca
    18661 ccagagcacc ggcagtaatc ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg
    18721 attagtctga tggattttcg ccgttacccc ttcattcagt gtcgcatgcc cgattcccgc
    18781 cagcagaata aattgcgcgg tagtgagcat aaacagcaaa cgtggcatat tccgcggttg
    18841 cataagggcg acgatcaacg ttacgagggc gagaacaatt tgccataacc agacgccacc
    18901 aaactgcgtt tgcaaaacag cctgccaaat gtttggcgaa aatacatctg cccatcccgt
    18961 tcccatcaac ccgccctgaa cggcgagcat tgcagtggca cagatcaaac tccagacggc
    19021 agcgtgctgt tgtaaacgca gaaaacgctt cgccagcaag cgacgaatcg ttaagggggc
    19081 cagccaggca ccgtacattg cgaagccaaa gaccagcatc agcgaggtga aatggataaa
    19141 tcgcagcgcg atccaggtaa acgccagcat gatttatttc acgctaaagg tgtagtgtcc
    19201 tttcgtttta tgcccgtcca ccgaaacaac atgccagtca acgatgtagg ttccgggttt
    19261 cagcgaatcg gccagtggaa caatcagttg cttttggtcc tgctcatttc gcttcgccgg
    19321 taatgtttta atattttcgt tttttggccc cgtgattttt gcaccactga atcctgtttc
    19381 aacaccttcc gagaagttta aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc
    19441 cgcaggatac tgatgcgtta aatgcgcatg tgcccagacg gaaggtgtca ccaacgaagt
    19501 ggtcaggatt gccagcgcgt agcgaaggga gcgtgcagtt gaagtcatat tatctattcc
    19561 tttttgtaat aactttttta cagagcataa ccttgtctaa tgtctgagtc gaggatcatc
    19621 aattccggct tgccatcctg gctcactctt agtaactttt gcccgcgaat gatgaggaga
    19681 ttaagaatgc tgaagaatct ggctaaactg gatcaaacag aaatggataa agtgaatgtc
    19741 gatttggcgg cggccggggt ggcatttaaa gaacgctaca atatgccggt gatcgctgaa
    19801 gcggttgaac gtgaacagcc tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc
    19861 caccgtttgg cttcggtcaa tctgtcacgt ttaccttacg agcccaaact taaataaaac
    19921 ttatacagag ttacactttc ttacataacg cctgctaaat tatgagtatt ttctaaaccg
    19981 cacccataat ttgcagtcat tttgaaaagg aagtcattat gtcgttctgg aaagttgcag
    20041 cagcgcaata tgagcctcgt aaaacctcac tcaccgaaca ggtagctcat catctggagt
    20101 ttgtgagggc cgccgccagg caacaatgtc agctgttagt ttttccatcg ctttctttac
    20161 tggggtgtga ttattcgcga cgtgcccttc ctgccccacc cgatctctca ctgttagacc
    20221 cgctttgtta tgccgcaacg acctggcgaa tgaccatcat tgccggcctt cccgttgaat
    20281 ataacgatcg ctttattcgt ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga
    20341 tctaccatca gagtcatggt gcatgtctgg gtcgtcgttc cagaaccatc acggtggtcg
    20401 atgaacaacc gcaaggtatg gatatggacc cgacctgttc actgtttact acagggcaat
    20461 gtctgggaga acccgacctg ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt
    20521 actcgattgc cgtgctgatg gcaaatgccc gtggtaacag tgcactatgg gatgaatatg
    20581 gtcgtctcat cgttcgcgcc gatcgtggtt cattattgtt agtcggtcag cgttcatcac
    20641 agggttggca aggcgatatc attccattac gctaggcttt ttcggcctgg agcatgccat
    20701 gttgcgcatt atcgatacag aaacctgcgg tttgcaggga gggatcgttg agattgcctc
    20761 tgttgatgtc attgacggaa aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg
    20821 tcctattagt ccacaagcga tggcgattca tcgcatcacc gaagccatgg tcgccgataa
    20881 accgtggatt gaagatgtga tcccacacta ttacggtagt gaatggtatg tcgcgcataa
    20941 cgccagcttt gaccgccgcg tactgcctga gatgcccggt gagtggattt gcactatgaa
    21001 actggcccgt cgtttgtggc ctgggatcaa gtacagcaat atggcgttat ataaaacacg
    21061 caagctcaat gtacagacgc cgccgggcct gcatcatcac cgcgcgttgt atgactgtta
    21121 tatcaccgcc gcgttgctta tcgatattat gaacacctcc ggctggacgg cagaacagat
    21181 ggccgatatc accggacgtc cgtcgttgat gacgaccttc acctttggca aataccgtgg
    21241 caaagcggtt tccgacgttg ccgaacgcga tccgggctat ctgcgctggt tatttaataa
    21301 cctggacagc atgagcccgg agctgcgttt aacactgaaa cattatctgg aaaatactta
    21361 gtccgcaggc gtagcgggta atgttccctg cgccagcgcg accagaaaag catattccat
    21421 cgctacgcct tcgtacgatt taaagcgacc agatttaccg ccatggcctg agtccatgtc
    21481 ggtacagagc aataaaagat ggtcatcggt tttcagctcg cgcaatttag cgacccattt
    21541 tgccggttcc caatattgca cctgagaatc gtgcaaaccg gtcgttacca gtaaatgcgg
    21601 ataagcctgt gcggtgacgt tgtcatacgg gctgtagctt ttcatgtact cgtaatattg
    21661 cggatcctgc ggattacccc actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc
    21721 aagcatcgtt gttacaacat caacaaacgg tacctgggcg ataacgccgt ggaataattc
    21781 cgggcgttga ttaattgcaa cgcccatcaa catgcccccc gcactcccgc ccatcgcata
    21841 acaaagcgaa ggagagccat agcccagttt taacaatgca tcgcaggcat caagataatc
    21901 attaaacgta tttttcttct tcagaaattt tccgtcttcg taccattgtt gccccagctc
    21961 accaccgccg cgaacatgga caatggcgta gacaaagcca cgatctaaca aactcaagtg
    22021 gctaaaactg aaatcggcat caatacttgc gccgtaagaa ccatagccat acaccagcaa
    22081 cgggttgtgt cctttgcgaa aatgtttgcg atggtagacc aacgaaaccg gaacttcgac
    22141 gccatcacgg gcgactatcc acaggtgttc actgcggtaa ttcgccgcat caaaaccggg
    22201 cacttctgtt tgttttaata cacgacgctc accggtatcc atatccagtt caaacaaagt
    22261 gtctggtgtg gtcatggaag aataaccata acgcaatcgc gcggtttcag gttctggatt
    22321 gtaggcaatc caggtcacat aggctggatc atcaaaggca ataccaatga cttcccgggt
    22381 cttgcggtta atttggcgca aactggttaa cccgcgctga cgctcttcaa ccaccagcca
    22441 gtcggtaaac agcgtaaacc cttccagcat gatgttttcg cgtggcggaa ttaactcttc
    22501 ccactgttgc tcatcacgca tacgggtacg gtataagcca aagtttttgc cgtggcggtt
    22561 ggaacgtaga taaaaacgat gctggtagtg atcaaggctg tattcgtgat ctttgcggcg
    22621 cggcagaaaa acaaacggct cggcatcggc catttccgcg tccagcaggc gaacttcact
    22681 ggtggtggcg ctggccaaat gaatgactac atagtgcttc gaggtcgttt tatgcaggct
    22741 gacgtaatag gtatcgtctt tttcttcgta gatcagttta tcttgcgatg ctggcgtacc
    22801 gatggcgtga cgccagacct gataaggcag cagcgtcacc ggatgcttgc gaacatagta
    22861 gaaagtccag gagtcatttg cccagacaaa gctgggttca acgttatcca gcagttccgg
    22921 gtaccagtta ccagtttcca gattacgaaa acgaatgccg tactggcgtc gggaaagaaa
    22981 atcttctgcc agcgccataa tggtgttatc gggcgtaatc gccattccgc ccatcgaata
    23041 aaactcacta tgagctgcgc gcttattggc atcgagcaat gtttcccact catcccactc
    23101 ttcactgaat gccgattgac gctggtagat agcatattca cagcctggtt cataaatatg
    23161 ccgatagcgg tagccatttt tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc
    23221 gatgatttcc tttaagatgc gatcctgcaa ggcttgttgt gaggccatca cccgatgacc
    23281 gtaactattt tcttgttgca ggtagtccag gacttctggc tgagaacgcg tatcgtcccg
    23341 cagccagtag taattatcga tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg
    23401 ggcggctttt ggtagcatgt tattgttctt tctggttgaa acatcttata agggtggcaa
    23461 aactcaccgg ggatgcaagc gaaacagggg agtcattgct tagatgatga caggtaatgg
    23521 cgcggatatc gaatgttatg cagacagaga aaatcagcct gttcaaatgg ctgtgcgatt
    23581 ctggatagcc cgaaatagtc aacttcaggc tatccagaga gcggaattat tccgccaaag
    23641 tgcgtttttg ctgttcgaga tcgcgttcaa tgccgtcacg aacatcctgg gggattttca
    23701 gcgcgtcacc cagtgcattc aggtaactgc gttccataaa atggtcaata tcaatagccg
    23761 cgcaactcag gaaatagatt tccagcgcct cttcttcatt gcggaccccg gtagccaggc
    23821 gttgtggatc cagcggttgt tcgattgcct gctcaatgag tacacgcccc tgctcttcca
    23881 cgccggcttc acgcaattgc tggtcgattg ccgcacgttc tttggcatca atatgaccat
    23941 cacttttagc ggcaaagacc agcgcaagga tcaaacgttc tgtacgctca tccagcggcg
    24001 tactttgcgc gccaaactgc ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt
    24061 tattccacag caccgtaccc gctaccgctc cgccgccaac cagtaacgcg ttggtgccat
    24121 atttcgtaag aagtttacgt gctgatttat ttgcgaccag cagcccagcc agtccgccta
    24181 atgcgcctgg cactaacagt ttgcccaatc cctgatccgc agacgaggag gtagaagaac
    24241 tgctttgccc aagcagggat tgcagttgat ttaaccagtt agccatattt gctcctcaat
    24301 aaccattttt agtgcctgta gcatagcgga tgaggatgtc agaaaatgta tatcgagaca
    24361 aaagatgcgc aaataagcgg ggcggtaggc cgttattcaa agaaattcgc gccaggtaag
    24421 gtatttactg tatatcaggt gataagggta ttttaggtga aagtagtttg tcgttttgag
    24481 tggcattgtt tgatgctgat tgcccgatgc gacgctgttg cgtcttctca ggcctacgat
    24541 tgtggtgcgt tctggggcgg acaaggcact cgcgccgcat ccgccaacgg tcaatcgaat
    24601 tatcagaagc cattaacgcc gctgatattc cgccattccc gctttgcagt ccacgctgac
    24661 ctggtaatga atatcggcac ttttaccacg cacggttagc ggtactgacc atttatcatc
    24721 tttaccctga atgtcctgca aactgaccca agccacggga tcggcctgac cgacaatttt
    24781 ttgatcatct gcccaacgcg ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc
    24841 aattccggca gcatccagac cttcacactt tgggaaagtg accgacttgc tggtttcatt
    24901 attggcagcg aaaactgatg cgcaggcaga aaccaacaac agccctaaaa acgcccctct
    24961 ttttttcatg tttttctcca tagcacaatg attcaggaga aagcatggta caaattgtca
    25021 ggagcgcaag ttgcttcagg ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac
    25081 gacctgtggg gacggtaatt tgccggtttt cagaatggtg ctcagcacat ctttttgttc
    25141 tgccagccat agcgaaaggg cttcacgttg ctcgtcttcc atttgcactg gagaatttgt
    25201 cagccaggtg tcaagcagat ccgccgtatc aagcatttta tcataagcat cggcttcctt
    25261 tttgctggta aacgacattt tctcttcgcc ctcacgaatg actacgtatt taacttcaac
    25321 cgccatttgc agcctctcat aataactgtg attttataca gtatatttct tttcggttga
    25381 gaaatcaaca tcagcaataa agacacacgc aaacgttttc gtttatactg cgcgcggaat
    25441 taatcagggg atattcgtta tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg
    25501 cgtgatgtta ttaggctccg gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct
    25561 cggcgtagag gtgattgccg tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca
    25621 tcgctcccat gtcattaata tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga
    25681 aaaaccacat tatatcgtgc cggagatcga agctattgcc accgatatgc tgatccaact
    25741 tgaagaggaa ggactgaatg ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg
    25801 cgagggtatc cgtcgcctgg cggcagaaca gctgcagctg cccacttcca cttatcgttt
    25861 tgccgatagc gaaagccttt tccgcgaggc ggttgccgcc attggctatc cctgcattgt
    25921 aaaaccggtg atgagctctt ccggcaaggg gcagacgttt attcgctctg cagagcaact
    25981 tgctcaggca tgggaatacg ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt
    26041 tgaaggcgtc gttaagtttg acttcgaaat taccctgcta accgtcagcg cggtggatgg
    26101 cgtccatttc tgtgcaccag taggtcatcg ccaggaagat ggcgactacc gtgaatcctg
    26161 gcaaccacag caaatgagcc cgcttgccct tgaacgtgcg caggagattg cccgtaaagt
    26221 ggtgctggca ctgggcggtt atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga
    26281 ggtgattttc agtgaggtct cccctcgtcc acatgatacc gggatggtga cgttaatttc
    26341 tcaagatctc tcagagtttg ccctgcatgt acgtgccttc ctcggacttc cggttggcgg
    26401 gatccgccag tatggtcctg cagcttctgc cgttatcctg ccacaactga ccagtcagaa
    26461 tgtcacgttt gataatgtgc agaatgccgt aggcgcaggt ttgcagattc gtttatttgg
    26521 taagccggca attaatggca gccgtcgtct gggggtggca ctggctactg cagagagtgt
    26581 tgttgacgcc attaaacgcg cgaagcacgc cgccggacag gtaaaagtac agggttaaac
    26641 ccggcaaaaa aacgctacaa aaatgcccga tccgcgatcg ggcattttga cttttacagc
    26701 ttagcgcctt ctacagcttc acgcgccagc ttagtaatac ggtcgtaatc gcccgcttcc
    26761 agcgcatctg ccggaaccag ccaggaacca ccgatgcaca gcacgctttt cagcgccagg
    26821 tagtcacggt agttagccgg agaaatacca cctgtcgggc agaaacggac ctgggagaac
    26881 ggacccgcga tcgcctgcag ggctttcacg ccgccgttag cttcagccgg gaagaatttg
    26941 aactctttca aaccgtagtc catacccagc atcagttcgg aaacagtgct gatccccgga
    27001 atcagaggaa tagtcccttc ggtagcagct ttcagcagcg gctcggtcag acccgggcta
    27061 attgcgaact gtgcacccgc ttcggtgact tctgccagct gctgtggatt cagcaccgta
    27121 ccggcaccca caatcgcttc aggcacttct ttggcgatag cacggatagc gtcaactgca
    27181 cactcggtac gcagagtcac ttccagaacg tgcaccccac cagcaaccaa cgcttttgcc
    27241 atcggcactg cgtgttccag tttttttacc acgataaccg gtacaaccgg gccggtggtc
    27301 aggattgatt ctgcacttgt tttccagttt ttcatcagag ttttctctcg cctgattaca
    27361 aatttgtcgt cttaaaaagt gatacaggtt gcgccctgtt cggcaccgga cagtttttca
    27421 cgcaaggcgc tgaataattc acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg
    27481 tgcggttcgc gagcagccag ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt
    27541 ccattcacac gaatgatgtc cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct
    27601 tctggtgtta cgtggatagc tgacggcact tttcctgaag cgccggagag tcgtccatcg
    27661 gtaactaacg caattttgaa acaccggtcc aataatacac caagtggcgg catgagttta
    27721 tgtaattctg gcattccgtt cgcttttggc ccctgatgac ggacaacaac gacacaatcg
    27781 cggtccagca aacccgcttc aaaggccggc ataacgtcat gctggctttc aaaaacaacc
    27841 gctggcgctt caatcacctg gttctcaacc ggcacggcag aggttttcat aaccgcacgg
    27901 cccaggttac cgcttaacac ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa
    27961 gcgatcacat tgctgtcgag tgatttttcc gccccttccc gccagtccag ttcaccatta
    28021 ttcagccatg gttcaagggt ataacgagac agaccaaagc ctgccaccgt attgacatct
    28081 tcatgcagca ggcctgcttt gagcagttca cgcaccagaa ccggtacgcc acctgccgcc
    28141 tggaagtggt taatatcggc cggaccgttc gggtagagac gtgccatcag cggtacaaca
    28201 tcagaaaggt cagagaagtc atcccagtta atctgaatac cggccgcgcg cgccatcgcc
    28261 accaggtgca tggtgtggtt agtggaacca ccggtcgcca gcagtgcaac gataccgttc
    28321 accaccactt tctcatcgat catcttaccg atcggcatcc attcattacc attaccggtc
    28381 atgcgtgtaa cctgacgcgc agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga
    28441 tgaacaaaag aagagcctgg caactgcatc cccataaatt ccaccaccat ctggttggtg
    28501 ttggcagtac cgtagaaagt acatgttccc ggcgcatggt aagacgcggc ttctgactcc
    28561 agtaaggcca tgcggtccac tttaccttcg gcataaagct ggcgaatacg cactttttct
    28621 ttatttggca aaccgcttgc catcggtcca gacggcacaa acaccgcagg caaatgacca
    28681 aacgacaggg ctgccatcgt cagacccggg acaatcttgt cgcacacacc gaggaacaga
    28741 gcaccatcaa acatgttatg ggacagcccg accgccgcag acatcgctat cacttcgcgg
    28801 cttagcagcg acaattccat tccatcctgc ccctgggtga caccatcaca catcgccgga
    28861 acaccgcccg caacctgacc aaccgcattc gcttcatgca gggctttacg aatgatttct
    28921 ggatagtgtt cataaggctg gtgcgcggag agcatgtcgt tataggaggt gatgatggcg
    28981 atattgttac gcaacatgct tttcaaagag gctttgtctt ctggctggca ggcagcgaaa
    29041 ccgtgtgcca ggttaccgca tgccaactgc gaacgatgaa tggtcgaagt tttcgcttgt
    29101 tctatccggg cgagataagc agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt
    29161 acgcgtaaca attgtggatt cataaaggct cctgaaattg agttgtcaga gcaggatgat
    29221 tcacaacgcg tttcattcag aggatttatg actgaaacgc ctgtaaccgg agctcatagg
    29281 gcaaaaacgt ttcagtcagt gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct
    29341 gaaagtgtaa aaattgttct acaatctgtg caagatcatg ttaccggtaa aataaccata
    29401 aaggataagc gcagatatta ctcaaactca ttccaggaac gaccatcacg ggtaatcatc
    29461 gccaccgagg caacgggtcc ccaggttccg gcctgatacg gtttcggcgc atcattgtcc
    29521 atcgcccacg cctcagtaat ggagtctacc catttccagg cttcttccac ttcgtcgcga
    29581 cgaacaaaca gtgcctgaat accacgcatg gtttccagca gcaaacgttc ataggcatcc
    29641 gccagatgcg tctgattaaa ggtttctgaa tagctcagat ccagcttggt gatttgcagg
    29701 ttatgtttgt ggtcaaggcc aggaacttta ttcagtacct ggatatccac gccttcatca
    29761 ggttgcagac ggatagtcag tttattctgc ggcagatcct gccacgattc tttaaacaga
    29821 ttcagttcag gtgttttgaa atagaccacg acttcagaac atttggtcgg cagacgttta
    29881 ccagtacgca ggtagaatgg cacaccggcc cagcgccagt tatcaatgtc gacgcggatc
    29941 gctacgaagg tttctgtatt gctgctcttg ttcgcgccct cttcttccag atatcccggc
    30001 acttttttgc cctgggcgaa gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg
    30061 ttggagcggt cgatgcggcg cagagacttc agtactttca ctttttcatc gcggatgctg
    30121 tctgcgctca ggtcagacgg cggagacatc gcaatcatgc agagaatttg cagcaggtgg
    30181 ttttggatca tatcgcgcat ctgaccggct ttatcaaaat agccccagcg cccttcgatc
    30241 cccacttctt ctgccacggt aatctcaaca tgatcaatgg tgcgattgtc ccagttattc
    30301 acaaacaggg agttagcaaa acgcagcgcc aacaggttca gcaccgtttc tttaccaaga
    30361 tagtggtcga tacggtaaac ctggcactcc tcgaagtatt cgccaacctg atcattgatt
    30421 tcctgcgagg tcgccagcga cgtccccagc ggtttctcca tgactacgcg tgccggttta
    30481 gcattcagtt tagcctcgcc aagccctttg caaattgcgc caaaagtgct gggcggcatg
    30541 gcaaagtagt taatggtgat acgatttttt tgatccagca tcacgccgag acggctgaat
    30601 gcagcagtgt cattgacatc gagattacaa aaatccagac gtgcactcag ggtgtcccat
    30661 aaaccttcat caatggtttc tttcatgaaa gtttcgagcg cctcgcggac aactttggta
    30721 tatgccgctt tatcccagtc agcacgccct acgccgataa tccgggtgtc cgggttgagc
    30781 tgaccggctt tttccagttg atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct
    30841 ttcgcgccga aaatgaccag gtcacaggcc tgggctgttt gcgttaccgc catgtcattc
    30901 tccttaagtt aactaacccg gtacttaagc cagggtatac ttgtaatttt cttacggtgc
    30961 actgtactgc ttttacgagc ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg
    31021 gtaaaagcgc gcaaagtgcg gcaaaactga taaaaaaatc atcgttttcc attgggtaaa
    31081 aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt
    31141 aacacagcac acaggcgtaa tatctgacaa aactacattt cgatttcttt cagtgcggaa
    31201 atcgtcatta cccgtgagtt tctttacatc atgaatatgc tggaaaaaat ccagtctcag
    31261 ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc ttcgcccgat
    31321 aacgcgatcc attcgagtat tgctgctctg gcgctggaag ccaatgttag cgaaccgacg
    31381 gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa acttcatctg
    31441 gcacaaagtc tggcgaatgg cactccctat gttaatcgca atgtcaatga agatgacagc
    31501 gttgaatcat acacagggaa aatatttgag tccgcaatgg caacgcttga tcatgtccgt
    31561 cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa
    31621 atcgcctttt tcggattagg ctcttcagcc gccgttgccc acgatgcgat gaataagttc
    31681 tttcgtttta atgttccggt ggtgtactcc gatgatatcg tgctgcaacg catgagttgt
    31741 atgaattgta gcgacggaga cgtggtggtg ctgatttctc acactggaag aacaaaaaat
    31801 ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcca tggtgattgc cctcacctct
    31861 gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt accggaagat
    31921 actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat agatgtgctg
    31981 gcgacaggat ttactttgcg acgcggtgca aaattcagag ataacttgaa gcgggtcaag
    32041 gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga cgatcgctaa
    32101 aaacgactgt cactgtccta atcttatacg acatccgaat gagattaatt tatcgccatc
    32161 gcggcgttat ttcattcgga tttcatgttc aagcaacact tggttgtttc agtcaacgga
    32221 gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt accacgttag gcccagcaac
    32281 agatcgcgat aataatcttg aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa
    32341 cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc gtgagattgc
    32401 cgcaaaactg gggcgtcatg tggctattct gggtgacctc caggggccca aaatccgtgt
    32461 atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc tgctcgacgc
    32521 caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc
    32581 tgctgacgtt gtgcctggcg acatcctgct gctggacgat ggtcgcgttc agttaaaagt
    32641 actggaagtt cagggcatga aagtgttcac cgaagtgacc gtcggtggtc ccctctccaa
    32701 caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga
    32761 caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg tctccttccc
    32821 acgctgcggc gaagatctga actatgcccg tcgcctggca cgcgatgcag gatgtgatgc
    32881 gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggatg caatggatga
    32941 catcatcctc gcctctgacg tggtaatggt tgcacgcggc gacctcggtg tggaaattgg
    33001 cgacccggaa ctggtcggca ttcagaaagc gttgatccgt cgtgcgcgtc agcttaaccg
    33061 cgcggtaatc acggcgaccc agatgatgga gtcaatgatt actaacccga tgccgacgcg
    33121 tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg tgatgctgtc
    33181 tgcagaaact gccgctgggc agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg
    33241 cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt
    33301 cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac gcagctaacc acctgaaagg
    33361 cgttacggcg attattacca tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat
    33421 cagctctggt ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga acctgactgc
    33481 tctctatcgt ggcgttacgc cggtgcactt tgatagcgct aatgacggcg tagcagctgc
    33541 cagcgaagcg gttaatctgc tgcgcgataa aggttacttg atgtctggtg acctggtgat
    33601 tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc gtattttaac
    33661 ggtagagtaa tctcacctgc cccggccgct gatgaccggg gtatttagca acacataaat
    33721 taggactact cattcaatcc aatcataatc acagccccct gtaaaatgaa tacagggata
    33781 gcctgaatgg agatctaaat agcttgcaag acgtcgcata cccgcttcct ctgcaacact
    33841 atatccaata actgccatcg gtaagccact atctttggag tattgtgtct gcacccatgt
    33901 tttaattgaa tcgttaatcg taa
//
LOCUS       DAZGTR010000041        33730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGTR010000041 DAZGTR010000000
VERSION     DAZGTR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.041"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..857)
                     /locus_tag="ACHLCY_002143"
     CDS             complement(<1..857)
                     /locus_tag="ACHLCY_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFP0369400.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHIYIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAA"
     gene            complement(1129..1290)
                     /gene="rsmS"
                     /locus_tag="ACHLCY_002144"
     CDS             complement(1129..1290)
                     /gene="rsmS"
                     /locus_tag="ACHLCY_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP0369401.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(1304..1831)
                     /gene="priC"
                     /locus_tag="ACHLCY_002145"
     CDS             complement(1304..1831)
                     /gene="priC"
                     /locus_tag="ACHLCY_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP0369402.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            1901..2278
                     /gene="ybaN"
                     /locus_tag="ACHLCY_002146"
     CDS             1901..2278
                     /gene="ybaN"
                     /locus_tag="ACHLCY_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP0369403.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            2431..2982
                     /gene="apt"
                     /locus_tag="ACHLCY_002147"
     CDS             2431..2982
                     /gene="apt"
                     /locus_tag="ACHLCY_002147"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP0369404.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            3111..5042
                     /gene="dnaX"
                     /locus_tag="ACHLCY_002148"
     CDS             3111..5042
                     /gene="dnaX"
                     /locus_tag="ACHLCY_002148"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP0369405.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    4375..4439
                     /gene="dnaX"
                     /locus_tag="ACHLCY_002148"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            5095..5424
                     /gene="ybaB"
                     /locus_tag="ACHLCY_002149"
     CDS             5095..5424
                     /gene="ybaB"
                     /locus_tag="ACHLCY_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP0369406.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            5424..6029
                     /gene="recR"
                     /locus_tag="ACHLCY_002150"
     CDS             5424..6029
                     /gene="recR"
                     /locus_tag="ACHLCY_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP0369407.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            6139..8013
                     /gene="htpG"
                     /locus_tag="ACHLCY_002151"
     CDS             6139..8013
                     /gene="htpG"
                     /locus_tag="ACHLCY_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP0369408.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            8194..8838
                     /gene="adk"
                     /locus_tag="ACHLCY_002152"
     CDS             8194..8838
                     /gene="adk"
                     /locus_tag="ACHLCY_002152"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP0369409.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            9074..10036
                     /gene="hemH"
                     /locus_tag="ACHLCY_002153"
     CDS             9074..10036
                     /gene="hemH"
                     /locus_tag="ACHLCY_002153"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP0369410.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKMYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(10033..10992)
                     /gene="aes"
                     /locus_tag="ACHLCY_002154"
     CDS             complement(10033..10992)
                     /gene="aes"
                     /locus_tag="ACHLCY_002154"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP0369411.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            11144..12448
                     /gene="gsk"
                     /locus_tag="ACHLCY_002155"
     CDS             11144..12448
                     /gene="gsk"
                     /locus_tag="ACHLCY_002155"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP0369412.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(12581..14257)
                     /gene="ybaL"
                     /locus_tag="ACHLCY_002156"
     CDS             complement(12581..14257)
                     /gene="ybaL"
                     /locus_tag="ACHLCY_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP0369413.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(14495..15715)
                     /gene="fsr"
                     /locus_tag="ACHLCY_002157"
     CDS             complement(14495..15715)
                     /gene="fsr"
                     /locus_tag="ACHLCY_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP0369414.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            15933..17585
                     /gene="ushA"
                     /locus_tag="ACHLCY_002158"
     CDS             15933..17585
                     /gene="ushA"
                     /locus_tag="ACHLCY_002158"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0369415.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGKMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGRLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(17622..18101)
                     /gene="ybaK"
                     /locus_tag="ACHLCY_002159"
     CDS             complement(17622..18101)
                     /gene="ybaK"
                     /locus_tag="ACHLCY_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP0369416.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(18305..19099)
                     /gene="ybaP"
                     /locus_tag="ACHLCY_002160"
     CDS             complement(18305..19099)
                     /gene="ybaP"
                     /locus_tag="ACHLCY_002160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP0369417.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            19237..19578
                     /gene="ybaQ"
                     /locus_tag="ACHLCY_002161"
     CDS             19237..19578
                     /gene="ybaQ"
                     /locus_tag="ACHLCY_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0369418.1"
                     /translation="MKQATRKPTTPGDILLYEYLVPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(19692..22196)
                     /gene="copA"
                     /locus_tag="ACHLCY_002162"
     CDS             complement(19692..22196)
                     /gene="copA"
                     /locus_tag="ACHLCY_002162"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP0369419.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            22458..23390
                     /gene="glsA"
                     /locus_tag="ACHLCY_002163"
     CDS             22458..23390
                     /gene="glsA"
                     /locus_tag="ACHLCY_002163"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP0369420.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     ATFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            23393..24685
                     /gene="ybaT"
                     /locus_tag="ACHLCY_002164"
     CDS             23393..24685
                     /gene="ybaT"
                     /locus_tag="ACHLCY_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0369421.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            24716..24813
                     /locus_tag="ACHLCY_002165"
                     /pseudo
     CDS             24716..24813
                     /locus_tag="ACHLCY_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24810..25217
                     /gene="cueR"
                     /locus_tag="ACHLCY_002166"
     CDS             24810..25217
                     /gene="cueR"
                     /locus_tag="ACHLCY_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP0369422.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(25218..25676)
                     /gene="ybbJ"
                     /locus_tag="ACHLCY_002167"
     CDS             complement(25218..25676)
                     /gene="ybbJ"
                     /locus_tag="ACHLCY_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP0369423.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(25673..26590)
                     /gene="qmcA"
                     /locus_tag="ACHLCY_002168"
     CDS             complement(25673..26590)
                     /gene="qmcA"
                     /locus_tag="ACHLCY_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0369424.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            26736..27413
                     /gene="fetA"
                     /locus_tag="ACHLCY_002169"
     CDS             26736..27413
                     /gene="fetA"
                     /locus_tag="ACHLCY_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP0369425.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTNGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            27400..28182
                     /gene="fetB"
                     /locus_tag="ACHLCY_002170"
     CDS             27400..28182
                     /gene="fetB"
                     /locus_tag="ACHLCY_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP0369426.1"
                     /translation="MNSHNITNESLALALMLVVVVAILISHKEKLALEKDIFWSVGRA
                     IIQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITV
                     GAGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEK
                     LSLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKY
                     QIMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(28245..29099)
                     /gene="cnoX"
                     /locus_tag="ACHLCY_002171"
     CDS             complement(28245..29099)
                     /gene="cnoX"
                     /locus_tag="ACHLCY_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP0369427.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(29160..29969)
                     /gene="ybbO"
                     /locus_tag="ACHLCY_002172"
     CDS             complement(29160..29969)
                     /gene="ybbO"
                     /locus_tag="ACHLCY_002172"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0369428.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(29959..30582)
                     /gene="tesA"
                     /locus_tag="ACHLCY_002173"
     CDS             complement(29959..30582)
                     /gene="tesA"
                     /locus_tag="ACHLCY_002173"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP0369429.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            30553..31239
                     /gene="ybbA"
                     /locus_tag="ACHLCY_002174"
     CDS             30553..31239
                     /gene="ybbA"
                     /locus_tag="ACHLCY_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP0369430.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            31236..33650
                     /gene="ybbP"
                     /locus_tag="ACHLCY_002175"
     CDS             31236..33650
                     /gene="ybbP"
                     /locus_tag="ACHLCY_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP0369431.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGQAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         7991 a         8929 c         9063 g         7747 t
ORIGIN      
        1 gccgccagac ggtcgcggaa cgcccgtccc cactcagtct tactggtggc taccattgcg
       61 ggcatataca acgccctgcg aagcgacaca tgtccggcct tactcatccg gctcgcccct
      121 ctcacactgc tacctgattc ataacgccgt ggtgtcagac ccgcaaaagc ggcgaactgt
      181 ctggcatggg cgaagcggtc cttcagaccg atataagcca gcaataccgc agatgttttc
      241 tctccgatac ccgggatgct ttccagcagt ttcctgcggt gtttcatatc cggatcatcg
      301 tctgtcaggt cttttatctg cttctcaaga cgcttcagct ctgcttcaag ccacagaagg
      361 tgagcatcaa tgctcggtct ctggacttcc cgcgccgttt cagtgcgatt cagttcctgc
      421 gtgtgcatat ctgtcagcgc ctggtggcgg actaccaggg cacgcaacgc gcgttcaagc
      481 gggtgaggcg cttcccaggc tgcagggcgc ttctgacgac agaactctgc cagcatgcgc
      541 gcatccacgg tatcagtctt gttacgcagt ccttcactct gagcgaaagc tttacccagc
      601 gcaggattaa tgactgacac tatgtagcca gcatcgtaaa ggcactcagc gacaggttcc
      661 atataggtgc cggtcgcttc gatgtagata tgcgcatggt caatcttgtg acctttcagc
      721 cagctcacca gctcatcgtg ccctttagtg gtgttagcga attttttggt gcgatgacga
      781 ccatcaggac gcaacacatc gacatccagt ttctctttag cggtgtcgat accgatataa
      841 tgaagttcat gttccatagt gaaaccaacc ttgcaaatac ggattaccgg gaaaaccggt
      901 ccatgatact gtccggttta tcactttggg agaaaggcag tccgctgcat aaatctacgc
      961 aacaggttag aacccaaggc ccgatacggc atgcggactg caggcagaga aaactggccg
     1021 attttctctg cactggaggg ataatacaag gcctgataag cgtagcgcat caggcagttt
     1081 tgcgtttgcc cacaatctta ggggacattt agcggcccca tttatttctc acttttccgc
     1141 ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt acaatatcca gcgcgcgcag
     1201 cacggtgcgg gctgggatct gattttcttc cagcagcaca atcaaatcga cagccagttt
     1261 gacatcgtca ggggcatttt ccagtgacat actctctcca ttgctagcgg gttaagcgcg
     1321 ctagcctgtt ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg
     1381 cttccacttc acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg
     1441 ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt
     1501 tacgctgcca gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg
     1561 aggcttcacg cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt
     1621 gcggcagctg ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcat
     1681 cgagacaagc ctgcagtgtt gtcgcacggg tttgaaaaag gtgtctgtca aaacgggcac
     1741 ttagcgtggc aaattgcgct accggggcac aacgctgacg cagcgtagcg agctgtcctt
     1801 ccagtttttc cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt
     1861 tatgctaaag tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat
     1921 catcattggc tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc
     1981 gacgacgccg tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca
     2041 cgcctggttg ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca
     2101 tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat
     2161 ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg
     2221 tttgcttttc tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag
     2281 cacaacaatc gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac
     2341 aggcgcaggc ggtcaaaggt taaacaactg ttacttttga tacgtttaaa acgcgccgtg
     2401 agtaccaccg taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat
     2461 ctcaaaaata gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat
     2521 gtcaccagct tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag
     2581 cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt
     2641 ggcgctccgg tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa
     2701 ctgccgcgtg aaaccatcag tgaaacttac gacctggaat acggcaccga tcagctggag
     2761 atccacgttg atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca
     2821 accggcggca ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct
     2881 gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag
     2941 ggcattacca gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc
     3001 tgcccacgct acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg
     3061 ttagcattac cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc
     3121 aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg
     3181 tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt
     3241 ccggtacccg tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact
     3301 gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc
     3361 aggggcgctt tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata
     3421 cccgcgacct gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc
     3481 tgatcgacga agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg
     3541 aagagccgcc ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg
     3601 tgacgatttt gtcgcgctgt ttgcaatttc atctcaaggc gctggatgtc gagcaaattc
     3661 gccatcaact tgagcacatc ctcaacgaag aacatatcgc tcacgagccg cgggcgttgc
     3721 aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg
     3781 cgattgccag cggtgacggc caggtttcaa cccaggcagt cagtgcgatg ctgggtacgc
     3841 ttgacgacga tcaggcgctg tcgctggttg aagcgatggt cgaggccaac ggcgagcgcg
     3901 taatggcgct gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg
     3961 aaatgctcgg cctgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca
     4021 acgacatggc cgccattgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg
     4081 atattcagct ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg
     4141 accgccgcat gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccacgtatgc
     4201 ctctgcctga gccagaagtg ccacgccagt cctttgcgcc cgtcgcgcca acggcagtaa
     4261 tgacgccaac ccaggtgccg ccgcaaccgc aatcagcgcc gcagcaggca ccgactgtac
     4321 cgctcccgga aactaccagc caggtgctgg cggcgcgcca gcagttgcag cgcgtgcagg
     4381 gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac ccgcgcgcgg ccggtgaata
     4441 acgctgcgct ggaaagactg gcttcggtca ccgatcgcgt tcaggcgcgt ccggtgccat
     4501 cggcgctgga aaaagcgcca gccaaaaaag aagcgtatcg ctggaaggct accactccag
     4561 tgatgcagca aaaagaagtg gtcgccacgc cgaaggcgct gaaaaaagcg ctggaacatg
     4621 aaaaaacgcc ggaactggcg gcgaagctgg cggcagaagc cattgagcgc gacgcgtggg
     4681 cggcacaggt gagtcaactt tcgctaccaa aactggtcga acaggtggcc ttaaatgcct
     4741 ggaaagagga gagcgacaac gcagtatgtc tgcatttgcg ctcctctcag cggcatttga
     4801 acaaccgcgg tgcacagcaa aaactggctg aagcgttgag cacgttaaaa ggttcaacgg
     4861 ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg tacgccgctg gagtggcgtc
     4921 aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc cattattgcg gataataata
     4981 ttcagaccct gcgtcggttc ttcgatgcgg agctggatga agaaagtatc cgccccattt
     5041 gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat tgagagagaa acctatgttt
     5101 ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc agatgcaaga aaaaatgcag
     5161 aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg aatctggcgc aggtctggta
     5221 aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag agatcgaccc gagcctgctg
     5281 gaagacgaca aagagatgct ggaagacctg gtggctgcag cattcaacga cgcagcacgt
     5341 cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat cctccggaat gcagctgccg
     5401 cctggcttta agatgccgtt ctgatgcaaa ccagcccgct gttaacacag cttatggaag
     5461 cactgcgctg tctgccgggc gttggcccga agtcggcgca gcgtatggcg ttcacgctgc
     5521 ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc gctcacccgg gcgatgtcgg
     5581 aaatcggcca ctgcgccgat tgccgcactt tcaccgaaca ggaagtctgt aacatctgtt
     5641 cgaatccgcg tcgtcaggaa aacggtcaaa tctgtgtggt ggagagtccg gcggacatct
     5701 acgccattga gcagacgggg cagttttcag gccgttattt tgtgttgatg gggcacctgt
     5761 caccgctgga cggcatcggt ccggatgata tcggccttga tcgtctggaa cagcgtctgg
     5821 cagaggaaaa aatcactgaa gtgatcctcg ccaccaatcc cacggttgaa ggtgaagcta
     5881 ccgctaacta cattgccgag ctttgcgcgc aatatgacgt ggaagccagc cgaatcgctc
     5941 atggcgtacc ggttggcggc gaactggaaa tggtcgatgg caccacgctg tcacactccc
     6001 ttgccgggcg tcataagatt cgtttttaag caaacgagag caggatcacc tgctctcgct
     6061 tgaaattatt ctcccttgtc cccatctctc cctcatcctg tttttaacct taaaatggca
     6121 ttattgaggt agacctacat gaaaggacaa gaaactcgtg gttttcagtc agaagtgaaa
     6181 cagcttctgc acctgatgat ccattctctc tattccaata aagaaatctt cctgcgtgag
     6241 cttatctcta acgcctccga tgcggcggac aagctgcgtt tccgtgcgct atctaacccg
     6301 gacctgtacg aaggtgatgg cgaactgcgc gttcgtgtct ctttcgataa agacaagcgc
     6361 acgctgacca tctccgataa cggcgtgggg atgacccgcg acgaagtgat tgaccatctg
     6421 gggactatcg ctaaatccgg taccaaatca ttcctcgaat ccctgggttc tgaccaggcg
     6481 aaagacagcc agctgatcgg tcagtttggt gttggtttct actctgcgtt tatcgtggcc
     6541 gacaaagtga ccgtgcgtac tcgcgcggca ggcgaaaaac cagaaaacgg cgtcttctgg
     6601 gaatcggctg gcgaaggtga atataccgtt gccgacatca ccaaagaaga tcgtggtact
     6661 gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc tcgatgactg gcgcgtgcgt
     6721 tccatcatca gcaaatactc cgaccatatc gcgctgccgg tagagatcga aaaacgcgaa
     6781 gagaaagacg gcgaaaccgt tatctcctgg gagaaaatca acaaagcgca ggcgctgtgg
     6841 actcgtaaca agtcggaaat caccgatgaa gagtacaaag agttctacaa acacatcgcc
     6901 cacgacttta atgatccgct gacctggagc cacaaccgcg ttgaaggtaa gcaggagtac
     6961 accagcctgc tgtacatccc gtcccaggct ccgtgggata tgtggaaccg tgatcataaa
     7021 cacggcctga aactgtacgt ccagcgtgtg ttcatcatgg acgacgcaga acagttcatg
     7081 ccgaactatc tgcgcttcgt gcgtggtctg attgactcca gcgatctgcc gctgaacgtt
     7141 tcccgtgaaa tcctccagga cagcacggta acgcgtaacc tgcgcaatgc gctgaccaag
     7201 cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg cggacaaata ccagaccttc
     7261 tggcaacagt ttggcctggt actgaaagaa ggtccggcgg aagatttcgc taaccaggaa
     7321 gcgatcgcca aactgctgcg ttttgcttct acttataccg attcttctgc gcagaccgtg
     7381 tctctggaag actacgtttc ccgaatgaaa gaagggcagg agaaaatcta ctacatcact
     7441 gcagacagct atgcggcagc gaagagcagc ccgcacctgg aactgctgcg taagaaaggc
     7501 atcgaagttc tgctgctttc cgaccgcatc gatgagtgga tgatgaacta tctgactgag
     7561 ttcgacggta aaccgttcca gtcggtgtct aaagttgacg agtcgctgga aaaactggct
     7621 gacgaagttg atgagagcgc gaaagaagcg gagaaagcac tgactccgtt catcgaccgt
     7681 gtgaaagccc tgctcggcga gcgcgtgaaa gatgtccgtc tgactcaccg tctgaccgat
     7741 acgccagcga tcgtttcgac cgacgcggac gaaatgagca ctcagatggc gaaactgttc
     7801 gctgcggcgg gccagaaagt gccagaagtg aaatacatct tcgaactgaa cccggatcac
     7861 gtactggtga aacgtgcggc agatactgaa gatgaagcga agttcagcga gtgggtagaa
     7921 ctgctgctgg atcaggcgct gctggcagaa cgcggcacgc tggaagatcc gaacctgttt
     7981 attcgtcgta tgaaccagct gctggtttcc tgatgtcatg ccggatgacc tttgtgtcat
     8041 ccggcatttt tcttttcatc atctgcactt tccgcaaatt atctcgccat taaccgtttc
     8101 agcctcaggt gcctttcttg aggcaatcgc ctgttggtgg tatcgtttat cgctttttca
     8161 aaaaattcga cacattttaa ggggattttc gcaatgcgta tcattctgct tggcgctccg
     8221 ggcgcgggga aagggactca ggctcagttc atcatggaga aatatggtat tccgcaaatc
     8281 tccactggcg atatgctgcg tgctgcggtc aaatctggct ccgagctggg taaacaagca
     8341 aaagacatta tggatgctgg caaactggtc accgacgaac tggtgatcgc gctggttaaa
     8401 gagcgcattg ctcaggaaga ctgccgtaat ggtttcctgt tggacggctt cccgcgtacc
     8461 attccgcagg cagacgcgat gaaagaagcg ggcatcaatg ttgattacgt tctggaattc
     8521 gacgtaccgg acgaactgat tgttgatcgt atcgtaggcc gccgcgttca tgcgccgtct
     8581 ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag aaggcaaaga cgacgttacc
     8641 ggtgaagaac tgactacccg taaagacgat caggaagaaa ccgtgcgtaa acgtctggtt
     8701 gaataccatc agatgactgc accgctgatc ggctactact ccaaagaagc ggaagcgggt
     8761 aacaccaaat acgcgaaagt tgacggcacc aagccggttg ctgaagttcg cgctgatctg
     8821 gaaaaaatcc tcggctaatt cgaaagcgcg cacggacagt cccctcgccc cctcggggag
     8881 agggttaggg tgaggggaac aggcccgcac aagcaaactt atcagcaatc tcaggccgga
     8941 tattcattcg gccttttaca aaaacctcaa tctgcgctat tcctttttct gatttgacct
     9001 ctcacaacaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
     9061 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
     9121 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
     9181 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
     9241 gcgctcgccg cgtgtggcga agatgtacgc ctcggtctgg atggaagacg gctcgccgct
     9301 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgcccgt
     9361 agcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
     9421 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
     9481 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
     9541 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
     9601 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
     9661 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
     9721 cgaactggct tccgcactgg ggatggcacc ggaaaaagtg atgatgacct ttcagtcgcg
     9781 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
     9841 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
     9901 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgccggcg ggaaaaaata
     9961 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    10021 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    10081 ggcggttttc atcattcgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    10141 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    10201 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    10261 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    10321 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    10381 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    10441 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    10501 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    10561 ataacaacaa gcagccacaa tttcctctat cgcttgcgga aaacgcgctt caggtgaaag
    10621 ggggtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    10681 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    10741 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    10801 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    10861 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    10921 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    10981 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    11041 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    11101 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    11161 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    11221 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    11281 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    11341 ggtgattgag gatgacgtag ccgaagcgct ttatcaggag ctaaaacaga aaaacctgat
    11401 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    11461 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    11521 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    11581 tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    11641 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    11701 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    11761 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    11821 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    11881 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    11941 tccgttgttg gcatctgaca aggcgctgga ctgggtagat ctggtgctgt gcaccgccgg
    12001 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    12061 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    12121 ccacaaggat tgccagaatc cgctgcgtgt atattcgcac attgcgccgt acatgggcgg
    12181 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    12241 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    12301 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    12361 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttaccggagc gtgaagacag
    12421 cctggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    12481 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    12541 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacctcac
    12601 ccgccggtgg cgtttccagc agttccagca tggtacgggc gatttcacgc tcgcccatca
    12661 ctacctgatt cgcaccacgt tcggtgatat acgccacttc atcgtcataa tgggcgcggg
    12721 caataatctc aatatccgga tttttcgcgc gggcagatgc cacaatctca cccgcttcat
    12781 aaccgttggg aatcgtcagg atcagccatt ttgcacattc cagatgcgcc agttgcataa
    12841 tttcttcgtt cgccgcattg cccaatactg cgcggacccc gcgctctcgc agctcatcaa
    12901 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    12961 ccagcaggct gcctacacga ccgtaaccca ccagtagcgc atggttgcaa atatccactg
    13021 ggatctgctt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    13081 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    13141 cccctgccag tactaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    13201 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    13261 tacgttggga gtgaccaaac agtcgcacca ggaaaaatgc ggctaacgac ttaccaaaca
    13321 gaataatcgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    13381 acaacatccc gacggagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcagcgtat
    13441 cgtgggcggc acggtgactc agttcagact cgttcagtac catcccggca aagaacgcac
    13501 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    13561 ccagcaccga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    13621 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc actttgccga
    13681 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    13741 ctgccactgc gggcagcaac accagcgtca gaaccattac caggtcttcc acaatcaacc
    13801 aaccgatggc gatttgccca cgctgactgt caattaattg ccgttcttca agtgcgcgca
    13861 gtaacaccac ggtactggcg gtggaaagac ataaaccgaa cacgataccg gtcattaacg
    13921 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    13981 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    14041 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    14101 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    14161 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    14221 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    14281 attgtccatt tttggccggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    14341 gatgatgttc atgaataatt attgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    14401 cacggatcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    14461 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    14521 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    14581 tactggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc ataccaaaag
    14641 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagcagc tcctgagcgt
    14701 aaaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    14761 ccgtccagtg caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    14821 agccccaaat cacatatttc cgcccaattt tatcccctac aggcccgccg atcaccgtac
    14881 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg agcattctgg atagataatc
    14941 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    15001 tcgagaaaat gaggattaac agaatgctga ccgccagtac cactttgttg cgcggcagtg
    15061 gattgataat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    15121 ggctgatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    15181 tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    15241 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    15301 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgccg
    15361 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    15421 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    15481 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    15541 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    15601 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    15661 tggtcgttga cgcagccgcg cccgccacag gctggggttg ttcactcatt gccataggaa
    15721 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    15781 acatacctgt atgcgtcgcc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    15841 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca tactattttt
    15901 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    15961 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    16021 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    16081 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    16141 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    16201 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    16261 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    16321 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    16381 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gtcattgggc
    16441 tgacaaccga tgacacagca aaaattggta acccggaata cttcactgat gtcgaatttc
    16501 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    16561 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    16621 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    16681 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    16741 tgccgggtac gccatgcaag ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    16801 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcaaa atgaaaatgg
    16861 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    16921 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    16981 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcgaa accaatggtc
    17041 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    17101 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    17161 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    17221 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    17281 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    17341 cgaaagacgg cagactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    17401 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    17461 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    17521 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    17581 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    17641 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    17701 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    17761 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    17821 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    17881 tttttaagat ccagttgacc ggcgaccggc gtaacggcca cggcaaggtg tttcatatca
    17941 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    18001 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    18061 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    18121 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    18181 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    18241 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    18301 ttttttagcg caacatttgc ggtaaattcc cttctccata caggtgtagt gcaccgaccg
    18361 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    18421 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atttcattat
    18481 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcattggtgt
    18541 gccagtgggt cagtgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    18601 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    18661 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    18721 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    18781 tgcccatctc ctggctaatg tgctgcagat tttggagttg ctcctcgcta atgcgctctt
    18841 ccagcgcctc gcaggcaggc aaattggcaa aaggcgtatc gctggtggaa acatccgcct
    18901 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    18961 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccgggaaggg
    19021 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    19081 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggggag
    19141 ggatggtgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatattctt
    19201 gtatcgctat tcacagggaa gcaacagtta aaaactatga aacaggcaac aagaaaaccg
    19261 acgacacccg gagatattct tctctatgaa tatctggtac cgctcgattt gaaaatcaat
    19321 gagttagcag agttgctgca tgttcatcgt aatagcgtca gtgcactgat caataacaat
    19381 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    19441 ttttggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    19501 caggaagaat tgggacggat tgaaacagtg gctgaatact tggcacgccg tgaagagcgt
    19561 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttt
    19621 acgccgcatc cggcagtcat gcgtcgatgc caaatgtgcc accctaaagc agcgcatccg
    19681 caatgacata cttattcctt cggtttaaac cgcagcaacc ggttggcgtt actcactacg
    19741 gtaatcgacg agagcgccat tgccgctccg gcaactaccg ggttaagcag tgttccagta
    19801 aacggccaca aaataccggc ggcgaccgga ataccgatac tgttgtagat aaaggcaccg
    19861 agcaggttct gcttcatgtt gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg
    19921 cccatcaggc tatggcgcat cagggtaatc gccgcggttt caatggcaac atcactgccg
    19981 ccccccatcg caataccgac atccgcctgg gccagcgctg gcgcatcgtt aatgccgtcg
    20041 cccaccattg ccacctgacg tccttcactt tgcaggcgtt taatcgcttc ggctttaccg
    20101 tccggcagca ccccggcgat cacttcatca atcccggctt ctttggcgat cgcattggcg
    20161 gtggttgggt tatccccggt caacatcacc agacgatatc ccgctttatg caggcgttgc
    20221 agcgccgcca cgctatcact acgcaatgga tcgcgtactg ccagcagggc taccgctttc
    20281 ccgtcaaccg ccagcagcac aggcgttgcc ccttgcgatg cctgagcagt aatctccgct
    20341 tcgatagctt tggtaccaac ctgttgctca tttaacagcg cctgattgcc cagcaataac
    20401 gcatgacctt cagcttcacc gctcacgccc agcccgcgca atgtgcggaa accgttgacc
    20461 tgcggcagct gcatatcacc tgctttatcg aggatcgccc gtgccagcgg atggctggaa
    20521 ccttgctcca gtgccgccgc cagacgcaat gcctgcgctt catcaacatc agcaaacgtt
    20581 ttcactgcga caacctgcgg cttcccttcg gtcagcgtcc cggttttatc gaacacgacg
    20641 gtatcgagtg tactggcgcg ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca
    20701 gccgcccgcc cgacgccgga aataatcgac atcggcgtcg ccagccccag cgcacacgga
    20761 caggcgataa tcagtaccgt ggtggcaatc accagggtat aaacaatttg cggtgccgga
    20821 ccaaagaagt accagattgc cgcactgacc agcgcaatca ccaccaccac aggtacaaat
    20881 acggctgaga ttttatccgc cagctgaccg atttctggct tgctgctctg ggcctggcgc
    20941 accatgcgaa tgatccgtga cagcgtggta tggctgccaa ccgcactggc acgaaacagc
    21001 acgctaccgt cctgtaccac tgtcccggca tggacgcttt cgccttcgcc tttttgctgc
    21061 gggattggtt cgcccgtcag catcgcttca tccagccatg cttcgccctg ggtaatctcg
    21121 ccatctaccg gcacgcgatc gccggtcgtc aggcgcagca acatacctgg ctgcacttcc
    21181 gccagaggca cgcttttttc accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa
    21241 tcgagtaatt tctccagcgc cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg
    21301 ccgagattga tcagaccgat aatcatcgcg ctggcttcgt aataaagatg tcgcgcttcc
    21361 atcgggaacc actgcggcca caggttgacg ctcatcgaat agagccacgc cacgccagta
    21421 cccagcgcca ccagcgtatc catcgtcgct gcaccgttca gcaggctttt ccatgcactg
    21481 cggtaaaaat ggccgccggc gaaaaccatc accgccaggg ttatcaggcc gataaccaac
    21541 cacaggctgc ggttgtcagc ggtgaccatc atgttatcgc caatcatccc ccagaccatc
    21601 accgggatac ccaccgccag tgcgacaatt gcctgccagc ggaagcgctt catcgtagca
    21661 acggcggttt cctgctggcg ctcacggcgt ttcgcgtcat cttcaatcgc ttccgcgccg
    21721 tagcccgctt tttccaccgc ctgcactaaa tcttgtgggg aggcactgcc catcaccagc
    21781 gcagtacgct ccgccaggtt tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca
    21841 ttttgtacgc gggtgacaca gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg
    21901 tcatcgtcat cggcggtcgc tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac
    21961 gggattgatg actccgccag cggtttagcc tttgggtggc ttacagatgc gtcataaccc
    22021 gcttgtttga tggtttcgat cagctgttct gcactggcag tcccggtaac gtgcgcttca
    22081 gtgatagaca catccgcttg ttccacatcc gggcgctgtt cgagactttc tttcacgcgt
    22141 ttaacgcagt gaccgcagga caggccgtcc agggtcaggt cgataatttg tgacataaaa
    22201 cactccttta agacagtttt gactggctgt gataaaggtt aaaccttcca gcaaggggaa
    22261 ggtcaagaaa ttaataaacc aggcgagtaa aagtccgtaa agattaaaaa attggctcaa
    22321 tttgcatcag gattagacat ttatctcttt gttttcctgt agttaagttg cgggtgctaa
    22381 gttaaatcaa gatgcctgaa aatcggcacc ggggtgagga attacctccc gcatctataa
    22441 aaaggagtta acaaaagatg ttagatgcaa acaaattaca gcaggcagtg gatcaggctt
    22501 acacccaatt tcactcactt aacggcggac aaaatgccga ctacattccc tttctggcga
    22561 atgtacctag tcaactggca gcagtggcta ttgtgacctg cgatggcaac atctatcgcg
    22621 cgggtgatag tgattaccgc tttgcgctgg aatccatctc gaaagtctgt acgttagccc
    22681 ttgcgttaga agatgtcggc ccgcaggcgg tacaggacaa aattggcgct gacccgaccg
    22741 gattgccctt taactcagtt atcgccttag agttgcatgg cggcaaacca ctgtcgccac
    22801 tggtaaatgc tggcgctatt gccaccacca gcctgattaa cgctgaaaat attgaacaac
    22861 gctggcagcg aattttacat atccaacagc aactggctgg tgagcaggta gcgctctctg
    22921 atgaagtcaa ccagtcggaa caaacaacca acttccataa ccgggccatt gcctggctgc
    22981 tgtactccgc cggatatctc tattgtgatg caatggaagc ctgtgacgtg tacacccgtc
    23041 agtgctccac gctcatcaat actgttgaac tggcaacgct tggcgcgacg ctggcggcgg
    23101 gtggtgtgaa tccgctgacg cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc
    23161 tggccgaaat gatgatggaa gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg
    23221 gtttaccggg caaaagcggt gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg
    23281 gaattgccac gttctcacca ccgctggacg aagaaggcaa cagtgttcgc ggtcaaaaaa
    23341 tggtggcatc ggtcgctaag caactcggct ataacgtgtt taagggctga tcatgatgaa
    23401 cacggaaggt aataacggta acaaacctct cggtctatgg aacgtcgttt ccatcggcat
    23461 tggggcaatg gtgggggcgg ggatcttcgc gctgctgggg caggctgcat tgctaatgga
    23521 agcctcgacc tgggtcgcct ttgcttttgg cggtattgtg gcgatgtttt ccggttatgc
    23581 ctatgcgcgt ctgggggcga gctatcccag caatggcggc attatcgact tctttcgtcg
    23641 cggattaggc aacggcgtct tttcgctggc gctctcgtta ctgtacctgt tgacgctggc
    23701 ggtgagcatc gccatggtcg cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga
    23761 aggcagccag gaggagcacc ttattttgct ctacgcgttg gggatcattg cggtgatgac
    23821 gcttttcaac tccttaagca accatgcggt agggcggctg gaagtgatcc tcgtcggcat
    23881 taaaatgatg atcctgttat tgctgattat tgccggtgtc tggtcgctgc aaccggcgca
    23941 tatttccgtc tctgcgcccc ccagctccgg tgcgttcttc tcctgtatcg ggataacctt
    24001 ccttgcctat gcgggctttg gcatgatggc gaacgcggcg gataaagtga aagatccgca
    24061 ggtcattatg ccgcgggcgt ttctggtggc gattggcgtt accacgttgc tttatatctc
    24121 gctggcactg gttttgctta gcgatgtatc ggcattagag ttagaaaaat atgccgatac
    24181 cgccgtagcg caggctgctt ctccgctgct cggacatgtg ggttatgtga tcgtcgtcat
    24241 cggcgcttta ctggcgacgg cttcagccat taacgcgaac ctgttcgccg tgtttaacat
    24301 catggacaac atgggcagcg aacgcgaact gccgaagcta atgaataaat ccctgtggcg
    24361 gcagagtacc tggggcaaca tcattgtcgt ggtgttgatt atgctgatga cggcggcact
    24421 aaatttaggc tcactcgcca gcgttgccag cgccaccttt ttgatttgct atctggcggt
    24481 gtttgtggtg gcgatccgcc tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt
    24541 tggtacgttg gtgatgttgt tggtgatcgt tggctttatc tacagtctgt ggtcccaggg
    24601 tagccgtgcg ttgatatgga ttattggctc actcttactc agccttattg tggcaatggt
    24661 catgaagcgc aataaaaccg tataacatct ctctgtgcgc agtacttcct gtattattgt
    24721 ggtggcggtc gatattcgca ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt
    24781 aacaaagcac aggaggcgtt gcgcgaacga tgaacatcag cgatgtagca aaaattaccg
    24841 gcctgaccag caaagccatt cgcttctatg aagagaaggg gctggtgacg ccgccgatgc
    24901 gcagcgaaaa cggttatcgc acctacacgc agcagcatct caacgaactg accttactgc
    24961 gccaggcacg gcaggtgggc tttaacctgg aagagagcgg cgagctggtg aatctgttta
    25021 acgacccgca gcggcacagc gccgacgtca aacggcgcac gctggagaag gtggcggaga
    25081 tcgaacgaca cattgaggag ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg
    25141 cctgccctgg cgatgacagc gccgactgcc cgattatcga aaatctctcc ggctgctgtc
    25201 atcatcgggc agggtgatta agacgagacg gctcggatat gtagggttat cccttctatc
    25261 gcaatgactt caacatgcgt acctgcaccg agatcctcgc tggcgctgac aggccatgaa
    25321 ctgtcaccga cgcgcatatg accgcgcccg ttgaccagcg gagattccag cacaaaacgt
    25381 cggccaatca gctgctgccc gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc
    25441 acccgccgcg acaaccattt ccaccacagc caggcggcga gcagcgtcag gatggcaaac
    25501 attacccctt gccactccca acccagcggc accagccaga ccaccaggcc ggtaatcacc
    25561 gctgccacgc cgctccacaa caaataacca tttccgccca gcatctcggc tgccagcagc
    25621 aaaccgccga gactgagcca gaaaatatgt ggatgaacga ccattaactc catcatggct
    25681 gagtccgttt gttggcgctg tctttcacca gctcggcaat cccggcaatc gaccccatca
    25741 ggctgctggc ctctaatggc atcatcacta ctttgctgtt actggaggaa ccgatctgct
    25801 gtaacgcttc ggtgtatttc tgcgctacga agtagttcac cgcctgaata tcaccggagg
    25861 cgatagcttc agacaccatt ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg
    25921 cttcagcctg taaaaacgcc gactgacgtt cgccttccgc tttcaggatt tgcgactgtt
    25981 tttcaccttc ggctttgagg atttccgcct gacggatccc ttccgcttca agaatgtaag
    26041 cgcgtttggt acgttccgct ttcatctgcg cgttcattga agagataagc tctgccggtg
    26101 ggcgcacgtc gcgaatttca atacgggtga ctttaatccc ccacgggttg gtggcttcat
    26161 cgacaatacg cagcaggcgt gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt
    26221 ccattgaacc caacacggta cggatgttag tcatggtcag gttgatgatc gccagctcca
    26281 gattgctgac ttcataagcc gcgcgcggcg cgtcaatcac ctgaataaaa cagactgcgt
    26341 cgatggtaac gttggcgtta tctttcgaga taacttcctg ggaagggata tcgagcactt
    26401 gctccatcat attgatcttg cgaccaatgc gatccataaa cggcaccacc agactgagcc
    26461 ccggctgtaa cgttttggta tagcgaccaa aacgttctac cgtccattga tagccctgcg
    26521 gcacgatttt gacgcccgcg ccgacaatga ccagcgcgac aaaaatgaga atcgggataa
    26581 agataagcat cggaaaaacc tcctgttgta ccgtccataa tcagcaaaat tgctgcttga
    26641 ttaaacaaat tatacctgat tactgaaaga gagttccccc ttattcctgc gaaggataaa
    26701 ctgtttttag taaaaatcag aaaaagggaa cagtgatgca ggaaaatagt cctttacttc
    26761 agctacaaaa cgtaggatat ctggcgggtg atgcgaagat tcttaataac atcaattttt
    26821 cgctgcgtgc tggcgaattt aagttaatta ccggtccttc cggttgtggc aaaagtacgc
    26881 tgctaaaaat agttgcttca ttgatcagcc caaccaacgg aacgttactg tttgaaggtg
    26941 aggatgtcag cacactaaag ccagaaatct accgccagca agtctcttac tgcgcccaga
    27001 cgccgacgct gtttggcgat acggtatacg ataatctgat ctttccctgg cagatccgta
    27061 accagcagcc tgacccggcc atttttctcg attttctcga acgcttcgct ttgccggaca
    27121 gcattttgac gaaaaatatc gccgagctat ctggtggtga aaaacaacgc atctcactga
    27181 ttcgtaacct gcaatttatg ccgaaggttt tattgctgga tgaaatcacc agtgcgctgg
    27241 atgaaagtaa taaacataac gttaatgaga tgatccatcg ttatgtgcgc gagcaaaata
    27301 ttgccgtgct gtgggtgaca cacgataaag acgaaattaa tcatgcggat aaagtgatta
    27361 cactgcaacc gcatgccgga gaaatgcagg aagcacgcta tgaactcgca taatattact
    27421 aacgaatcat tggcactggc attaatgctg gtggtggtgg tggcaatctt aattagccat
    27481 aaagaaaaac tggcgctgga gaaagatatt ttctggagcg tcgggcgagc gataattcag
    27541 ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc cagcctgaca
    27601 ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa
    27661 tatattgcta aagcttttat ctcatcgttt attgccatta cggtcggggc gggaattacc
    27721 ctggcggtgc tgattctctc tgggtcgatt gaatttatcc cgatgcaggt gatccctatc
    27781 gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa caatttaggg
    27841 caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg tgcgacgccg
    27901 aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat tccgacggtc
    27961 gattcagcaa aaacggttgg cttagtgagt ttacctggaa tgatgtccgg gctgatattt
    28021 gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt tatgctgctc
    28081 tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa gttttataat
    28141 tcgcgccacc agttggtggt gacgcaattg aagaagaaat gatgcgatgc cggatgcgca
    28201 gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata caacaatgca
    28261 tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc agcgaggatc
    28321 tcctgaaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg cagatgcccg
    28381 aacagcaact ccagcgcctc ttcattgcgc ccaacctggt gcagttgcag agccagttgc
    28441 gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg ttgaatttcc
    28501 ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg gtagcgggtg
    28561 tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga acggttcagc
    28621 gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga caactgccag
    28681 gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc
    28741 tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc gcggatcgcc
    28801 tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa cagatacacg
    28861 gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc gcagtccagc
    28921 ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat cggggttaac
    28981 tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac
    29041 tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc tacggacatg
    29101 gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg cgcttcaact
    29161 cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttgag caccattact
    29221 gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat aaaagcatgg
    29281 cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat gccgggattt
    29341 tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt acgaatggga
    29401 ccgggttcga tcaggctgac tttaattccg ctgtggcgca gctccatgcg cagtgcgtct
    29461 gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg cgtggagatt
    29521 aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa catcgcgggt
    29581 aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc
    29641 tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt gttaaagatc
    29701 ccatacagac aattatcggt cagggcgatc acctcgtctg ctgcgcgatc aacactttct
    29761 ggtgaatcca gatcgatcaa cacgccggta aatcccatgt tgttcatacg ctcaacatca
    29821 tccggttttc ggcaacctgc cagcacatga aaaccctggc gttttaattc gagcgcgctt
    29881 tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat aactttacct
    29941 gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact gcttcgccat
    30001 ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat cctgcatcca
    30061 ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat caaactcttt
    30121 ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat agtttgcagg
    30181 cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct gcaaaatctg
    30241 gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat tgccgcccag
    30301 ttcaaccagc acccagcgcg gctgatgctg tttcagcaga gccggaaggc gcgccagtcc
    30361 ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac tctgccactt
    30421 atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatacccgg cgctcaggct
    30481 atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa
    30541 caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc
    30601 ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt caaacgtggc
    30661 gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct ggcgatcctc
    30721 gccgggcttg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc gctacataat
    30781 atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt ttttcagtca
    30841 tttatgttaa ttcctaccct taacgcgctg gaaaatgtcg agcttccggc tctgctgcgc
    30901 ggtgagagta gcgcggaaag tcgtaacggg gcgaaagcgt tgctcgaaca gttagggctg
    30961 ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca acgagtggcg
    31021 ctggcacgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc caccggcaac
    31081 cttgaccgcc agacgggcga taaaattgcc gacctgctgt tttccctcaa ccgtgaacat
    31141 ggcaccacgt tgattatggt gactcacgac ctgcaactgg cggcacgctg cgaccgctgc
    31201 ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg ttctggcgcg
    31261 aatggcgctc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg gtggcctgcg
    31321 tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccag caaagccgtg
    31381 agtttatggc gggcgatcgg gcgttgcgca gttcgcgcga agtgccgcaa gcatggctgg
    31441 aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc accatgacat
    31501 ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc tacccgatgt
    31561 atggcgatct gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg
    31621 ccccacgcct gatggcactg cttaacctga aaacgggcga caccattgac gtgggcgatg
    31681 ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt aaccccttcc
    31741 agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga gccgtgcagc
    31801 cggggagtcg ggtcacctgg cgttataaat tcggcggcaa cgagaaccag ctcgacggct
    31861 atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt ctggaacagg
    31921 acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg ctttcggcgc
    31981 ttctgacctt gctgctggca gtggcagcgg tggcggtagc gatgaatcat tactgtcgca
    32041 gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcaagcg caactgcgta
    32101 agctaatcgt cggtcagtgg ttgatggtgc tggcgctttc agccgttacc ggtggggcga
    32161 taggcctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac
    32221 taccgccagc cagcctctgg ccgtggctgt gggcgctggg taccatgacg gtcatctcgc
    32281 tgctggtggg gctacgaccg taccgcctgt tgctggcaac gcagccttta cgcgtattac
    32341 gtaatgatgt ggtagcgaac gtctggccgc tgaagtttta tctgccgatt gtcagtgtgg
    32401 tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg gcggtgctgg
    32461 cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg ctgaatgtac
    32521 ttcgccgcat gacgctgaaa tcgctacctc tgcgcctggc ggttagccgc ctgttacgtc
    32581 agccgtggtc aacattaagc cagctttcgg cattttcgct ctcctttatg ctgctggcgc
    32641 tgttgctggt attgcgtggc gatctgcttg accgctggca gcagcagcta cctccagaaa
    32701 gcccgaacta ctttttaatt aacattgcca cagaacaggt agaaccgcta aaagcgttcc
    32761 tcgcggaaca tcagatagtc ccggaatcat tttatccggt agtgcgggcg cggctgacgg
    32821 cgattaacga taagccgaca gaaggtaatg aagatgaggc gcttaaccgc gaactcaatc
    32881 ttacctggca aaatacgcga cccgatcata atccgattgt cgccggtaac tggccgccaa
    32941 aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg
    33001 ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc agcctgcgca
    33061 aagtggactg ggaaagtctg cggcctaatt tctattttat tttccctgaa ggggcattag
    33121 acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac ggcatgttga
    33181 cacaactcaa ccgccagttc ccgaccatta gcctgttaga tattggcgcg attttaaaac
    33241 aggtcggtca ggtgctggag caggtaagtc gggcgctgga ggtgatggtg gtgctggtca
    33301 ccgcctgcgg tatgttgctg ttgctggcac aggtgcaggt gggaatgcgc cagcgtcatc
    33361 aggaactggt ggtatggcgc acactcggtg cggggaaaaa actgctgcgc acgacattgt
    33421 ggtgtgagtt cgccatgctt gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa
    33481 cggcactggc agtgttgcag gcgaaagtgt ttgatttccc gtgggagcca gactggcgat
    33541 tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg
    33601 gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga tgagagtaaa
    33661 taaggggcgt tctggttgaa tggaacgcct tgtttaaatt aattctacaa tatgtttcat
    33721 tgatgatgtt
//
LOCUS       DAZGTR010000042        32384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGTR010000042 DAZGTR010000000
VERSION     DAZGTR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.042"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(76..1836)
                     /gene="ycaO"
                     /locus_tag="ACHLCY_002176"
     CDS             complement(76..1836)
                     /gene="ycaO"
                     /locus_tag="ACHLCY_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP0369432.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1966..2658
                     /locus_tag="ACHLCY_002177"
     CDS             1966..2658
                     /locus_tag="ACHLCY_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP0369433.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2857..3945
                     /gene="serC"
                     /locus_tag="ACHLCY_002178"
     CDS             2857..3945
                     /gene="serC"
                     /locus_tag="ACHLCY_002178"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP0369434.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4016..5299
                     /gene="aroA"
                     /locus_tag="ACHLCY_002179"
     CDS             4016..5299
                     /gene="aroA"
                     /locus_tag="ACHLCY_002179"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP0369435.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5468..6232
                     /gene="ycaL"
                     /locus_tag="ACHLCY_002180"
     CDS             5468..6232
                     /gene="ycaL"
                     /locus_tag="ACHLCY_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP0369436.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6405..7088
                     /gene="cmk"
                     /locus_tag="ACHLCY_002181"
     CDS             6405..7088
                     /gene="cmk"
                     /locus_tag="ACHLCY_002181"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP0369437.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7199..8872
                     /gene="rpsA"
                     /locus_tag="ACHLCY_002182"
     CDS             7199..8872
                     /gene="rpsA"
                     /locus_tag="ACHLCY_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP0369438.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9032..9316
                     /gene="ihfB"
                     /locus_tag="ACHLCY_002183"
     CDS             9032..9316
                     /gene="ihfB"
                     /locus_tag="ACHLCY_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP0369439.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9523..11787
                     /locus_tag="ACHLCY_002184"
     CDS             9523..11787
                     /locus_tag="ACHLCY_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP0369440.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMHD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVYLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11824..13572
                     /gene="msbA"
                     /locus_tag="ACHLCY_002185"
     CDS             11824..13572
                     /gene="msbA"
                     /locus_tag="ACHLCY_002185"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP0369441.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13569..14555
                     /gene="lpxK"
                     /locus_tag="ACHLCY_002186"
     CDS             13569..14555
                     /gene="lpxK"
                     /locus_tag="ACHLCY_002186"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP0369442.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14592..15824
                     /gene="ycaQ"
                     /locus_tag="ACHLCY_002187"
     CDS             14592..15824
                     /gene="ycaQ"
                     /locus_tag="ACHLCY_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP0369443.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15876..16058
                     /gene="ycaR"
                     /locus_tag="ACHLCY_002188"
     CDS             15876..16058
                     /gene="ycaR"
                     /locus_tag="ACHLCY_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP0369444.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16055..16801
                     /gene="kdsB"
                     /locus_tag="ACHLCY_002189"
     CDS             16055..16801
                     /gene="kdsB"
                     /locus_tag="ACHLCY_002189"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0369445.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16955..17848
                     /locus_tag="ACHLCY_002190"
     CDS             16955..17848
                     /locus_tag="ACHLCY_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP0369446.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17825..18604)
                     /gene="elyC"
                     /locus_tag="ACHLCY_002191"
     CDS             complement(17825..18604)
                     /gene="elyC"
                     /locus_tag="ACHLCY_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP0369447.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18740..19525
                     /gene="cmoM"
                     /locus_tag="ACHLCY_002192"
     CDS             18740..19525
                     /gene="cmoM"
                     /locus_tag="ACHLCY_002192"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP0369448.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19522..20844
                     /gene="mukF"
                     /locus_tag="ACHLCY_002193"
     CDS             19522..20844
                     /gene="mukF"
                     /locus_tag="ACHLCY_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP0369449.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20825..21529
                     /gene="mukE"
                     /locus_tag="ACHLCY_002194"
     CDS             20825..21529
                     /gene="mukE"
                     /locus_tag="ACHLCY_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP0369450.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21529..25989
                     /gene="mukB"
                     /locus_tag="ACHLCY_002195"
     CDS             21529..25989
                     /gene="mukB"
                     /locus_tag="ACHLCY_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP0369451.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLANYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQQQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQITEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTTLNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26250..28097
                     /gene="ldtD"
                     /locus_tag="ACHLCY_002196"
     CDS             26250..28097
                     /gene="ldtD"
                     /locus_tag="ACHLCY_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0369452.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYKNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28278..28826
                     /locus_tag="ACHLCY_002197"
     CDS             28278..28826
                     /locus_tag="ACHLCY_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP0369453.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28853..29500
                     /gene="gloC"
                     /locus_tag="ACHLCY_002198"
     CDS             28853..29500
                     /gene="gloC"
                     /locus_tag="ACHLCY_002198"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP0369454.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29722..30912)
                     /gene="aspC"
                     /locus_tag="ACHLCY_002199"
     CDS             complement(29722..30912)
                     /gene="aspC"
                     /locus_tag="ACHLCY_002199"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP0369455.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31097..32185)
                     /gene="ompF"
                     /locus_tag="ACHLCY_002200"
     CDS             complement(31097..32185)
                     /gene="ompF"
                     /locus_tag="ACHLCY_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP0369456.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7882 a         7864 c         8719 g         7919 t
ORIGIN      
        1 gcggggcatt aaaaaaacgc cccgtaatat aactcagact aatcattaag cctacattgc
       61 gtaggctatt tgattttatt ttgcccagaa tgctgctttg gcgcgctgca gcttttcgta
      121 ggccttcaac aacgactgat gtgcagcaaa cgcgtgcaga tcgctatcta ccggttgcag
      181 gccgtaaaac gccgcttcgc cgctcattgc cgcactggcg gcttctacgg catctgcgcc
      241 gtacatgcga acaaacgcat tcagatattg cagaggctgg cgatcttctt cctgtgccag
      301 taataacaac gtttgcaggc agcgataata gttggcgcgt tccggactaa atactgatga
      361 gttaaactcc atcgtccatt cggtccagac cagagcctgt tccagatcgc caccagccag
      421 cgccagcatg gcttttaatt caccgatacg cagggtgtac caaccgttat ccgacccggt
      481 cgccagaccc aacagctcac gcacgcgggt aaagtcatca aaaccttctt catccagttg
      541 ctcgatgagg ttcaggtaat cttctttttc ccactcgctg cctggtagcg aaagaatcgt
      601 ttcacgtaaa tggctgccca tactgttatt cgcgagccac agatcttcag ccggataaat
      661 atcggacatg ccaggcacga taatacggca agcataaacg cccagatgct cgtaatcggc
      721 aatataaact tctttatctt ctttgttgaa gatagccatc agcgtagcaa actcttcttc
      781 cgtggtgccg gagaaattcc agtccacaaa cggataatcg gcatcctgct tgaacaggtc
      841 ccaggagatt aaaccgctgg aatcgataaa gtgcgtttcg aggttggtat gttcagcgac
      901 ttcttcatca tcgaaggttg gcggagtaaa cacatccaaa tctttcaggc cacgaccttg
      961 cagcagctcg gtcacggtac gttccagtgc tacgccaaaa tcaggatgcg caccgaaaga
     1021 ggcaaagcag gtaccgttag caggattgaa cagtaccacg caaatcaccg gatactggcc
     1081 gccaagcgaa ccgtcataag cgaagattgg gaaaccctcc gcttccagcg tttcgatggc
     1141 ttcaactact gctgggtaac gcgccagcac gtctgccggg atctctggca ggctgatgct
     1201 ttcagcaata atgcggtttt tcacgtagcg ttcgaaaact tcagacaatc cctgaacgcg
     1261 tgcttcgttg cgggtattac ctgcggacat accgttggag acgtacaggt taccaatgat
     1321 attcatcgga atataaacgg tctgattgtc ggactgacgc gtaaacggca ggccgcaaat
     1381 accacgatct tcgttaccgg attgtaggtc aatcagcatg ctgccggtca gttcattctc
     1441 cggatcataa aacgcgcgca gacggtcatc gagtagtcct tctggcacat cgtcattttc
     1501 ggtcagtggg aaccattttt cgttgggata atgcacgaac ggaccgttgg cgatggtttc
     1561 gcccagccag aaatcagcaa aaaagtagtt ggttgagaga cgctcgaaat attcaccaag
     1621 tgcagaagcc agcgccgctt ttttggttgc gccttttccg ttggtaaaac acagtgcgca
     1681 ctctttgtcg cgaatatgta cagaccagac gttaggcaca ggattcagcc aggaggcctc
     1741 ttcaatctga aagccgaggt ctgaaagttt ttgctggaag cgagcgatgg aatcttccag
     1801 agcggcatct ttgccgggga taaatgtttg cgtcatgaaa atcactttag tcgtacggaa
     1861 agcgcgcaat aatacgggtt ttatctcaaa ggcgctatca ccgccgccat gccgatgaac
     1921 tgttgactat gcttttagcg gataaaccac gataagagca taaaaatgaa ggcgttcgat
     1981 ctccaccgta tggcatttga taaagtgcct tttgatttcc ttggcgaagt tgcactacgt
     2041 agtctttata cctttgtact ggtttttttg ttcctcaaaa tgaccggaag acgcggtgtg
     2101 cgacagatgt cgctgtttga agtgttaatc attctcacgc tgggttcagc ggcgggagat
     2161 gtggcgtttt atgatgatgt gccgatggtg ccggtactta tcgtctttat cactctggcg
     2221 ttgttatacc gcctggtaat gtggttgatg gcgcacagtg aaaaactgga agatctactg
     2281 gaaggtaagc cggttgttat tattgaagat ggcgagctgg cctggtcgaa actcaataac
     2341 tccaacatga cggaatttga gttctttatg gagctacgat tgcgtggcgt ggagcagctg
     2401 gggcaggtac gtctggcgat tctcgaaacc aacgggcaaa tcagtgtcta tttctttgaa
     2461 gatgacaagg tgaaaccggg tttacttatt ttacccagtg attgtacgca gcgttacaaa
     2521 gtggtgccgg agtcggcgga ctatgcctgt attcgttgta gtgaaatcat tcatatgaaa
     2581 gcgggggaaa aacaattatg tccgcgctgt gcaaatccag aatggacgaa ggcaagtcgg
     2641 gcaaaacggg tgacctgaca gtaaaaacat cggctttttg ctaataatcc gagagattct
     2701 tttgtgtgat gcaagccaca tttttgccct caacggtttt actcattgcg atgtgtgtca
     2761 ctgaatgata aaaccgatag ccacaggaat aatgtattac ctgtggtcac aatcgattga
     2821 ccgcgggtta atagcaacgc aacgtggtga ggggaaatgg ctcaaatctt caattttagt
     2881 tctggtccgg caatgctacc ggcagaggtg cttaaacagg ctcaacagga actgcgcgac
     2941 tggaacggtc ttggtacgtc ggtgatggaa gtgagtcacc gtggcaaaga gttcattcag
     3001 gttgcagagg aagccgagaa ggattttcgc gatcttctta atgtcccctc caactacaag
     3061 gtattattct gccatggcgg tggtcgcggt cagtttgctg cggtaccgct gaatattctc
     3121 ggtgataaaa ccaccgcaga ttatgttgat gccggttact gggcggcaag tgccattaaa
     3181 gaagcgaaaa aatactgcac gcctaatgtc tttgacgcca aagtgactgt tgatggtctg
     3241 cgcgcggtta agccaatgcg tgaatggcaa ctctctgata atgctgctta tatgcattac
     3301 tgcccgaatg aaaccatcga cggtatcgcc atcgacgaaa cgccagactt cggcaaagat
     3361 gtggtggtcg ccgccgactt ctcttcaacc attctttccc gtccgattga cgtcagccgt
     3421 tatggcgtga tttacgctgg cgcgcagaaa aatatcggcc cggctggcct gacaatcgtc
     3481 atcgttcgtg aagatttgct gggcaaagcg aatatcgcgt gtccgtcgat tctggattat
     3541 tccatcctca acgataacgg ctccatgttt aacacgccgc cgacatttgc ctggtatcta
     3601 tctggtctgg tctttaaatg gctgaaagcg aacggcggtg tagctgaaat ggataaaatc
     3661 aatcagcaaa aagcagaact gctatatggg gtgattgata acagcgattt ctaccgcaat
     3721 gacgtggcga aagctaaccg ttcgcggatg aacgtgccgt tccagttggc ggacagtgcg
     3781 cttgacaaat tgttccttga agagtctttt gctgctggcc ttcatgcact gaaaggtcac
     3841 cgtgtggtcg gcggaatgcg cgcttctatt tataacgcca tgccgctgga aggcgttaaa
     3901 gcgctgacag acttcatggt tgagttcgaa cgccgtcacg gttaatgccg aaattttgct
     3961 taatccccac agccagcctg tggggttttt atttctgttg tagagagttg agttcatgga
     4021 atccctgacg ttacaaccca tcgctcgtgt cgatggcact attaatctgc ccggttccaa
     4081 gagcgtttct aaccgcgctt tattgctggc ggcattagca cacggcaaaa cagtattaac
     4141 caatctgctg gatagcgatg acgtgcgcca tatgctgaat gcattaacag cgttaggggt
     4201 aagctatacg ctttcagccg atcgtacgcg ttgcgaaatt atcggtaacg gcggtccatt
     4261 acacgcagaa ggtgccctgg agttgttcct cggtaacgcc ggaacggcaa tgcgtccgct
     4321 ggcggcagct ctttgtctgg gtagcaatga tattgtgctg accggtgagc cgcgtatgaa
     4381 agaacgcccg attggtcatc tggtggatgc gctgcgcctg ggcggggcga agatcactta
     4441 cctggaacaa gaaaattatc cgccgttgcg tttacagggc ggctttactg gcggcaacgt
     4501 tgacgttgat ggctccgttt ccagccaatt cctcaccgca ctgttaatga ctgcgcctct
     4561 tgcgccggaa gatacggtga ttcgtattaa aggcgatctg gtttctaaac cttatatcga
     4621 catcacactc aatctgatga agacgtttgg tgttgaaatt gaaaatcagc actatcaaca
     4681 atttgtcgta aaaggcgggc agtcttatca gtctccgggt acttatttgg tcgaaggcga
     4741 tgcatcttcg gcttcttact ttctggcagc agcagcaatc aaaggcggca ctgtaaaagt
     4801 gaccggtatt ggacgtaaca gtatgcaggg tgatattcgc tttgctgatg tgctggaaaa
     4861 aatgggcgcg accatttgct ggggcgatga ttatatttcc tgcacgcgtg gtgaactgaa
     4921 cgctattgat atggatatga accatattcc cgatgcggcg atgaccattg ccacggcggc
     4981 gttatttgca aaaggcacca ccacgctgcg caatatctat aactggcgtg ttaaagaaac
     5041 cgatcgcctg tttgcgatgg caacagaact gcgtaaagtc ggtgcggaag tagaagaggg
     5101 gcacgattac attcgtatca ctccaccgga aaaactgaac tttgccgaga tcgcgacgta
     5161 caatgatcac cggatggcga tgtgtttctc gctggtggcg ttgtcagata caccagtgac
     5221 gattcttgat cccaaatgta cggccaaaac atttccggat tatttcgagc agttggcgcg
     5281 gattagccag gcagcctgaa tgaacaacgg gcaataaata gccaaatctt tctttatcaa
     5341 aacgtcggca cattgtcggc gttttttttc ggaccttgtg agtcattttg attaatggta
     5401 gcgtcgcttg tcaatgtaag ttgttgatac ataatattta tatatgatta atcaacggat
     5461 gattcacatg aagaatacta aattactgct ggcgattgcg acctctgcag cattactgac
     5521 agggtgtcaa aatacccacg gtattgatac caatatggct atcagctccg gtttaaatgc
     5581 ctataaagca gcaacattaa gcgatgccga tgcaaaagcg attgccaatc agggctgtgc
     5641 cgaaatggac agcggcaatc aagtcgcaag taaatccagc aagtacggta aacgtctggc
     5701 aaaaatcgcc aaagcattgg gtaacaatat taatggcacg ccggtcaact ataaggttta
     5761 tatgaccagc gacgtcaacg catgggcgat ggcgaacggc tgtgtccgtg tctacagtgg
     5821 cctgatggac atgatgaatg acaacgaaat tgaaggtgtt ctgggccatg aactgggcca
     5881 cgtcgcgttg ggtcactcgc tggctgaaat gaaagcttct tatgcgatcg ttgccgcacg
     5941 cgatgccatt tcagctacca gcggtgtggc ttcccagctt tcccgctcac aattgggtga
     6001 tatcgcagaa ggcgctatta atgctaaata ctcccgtgat aaagagtccg aagcagatga
     6061 tttctccttt gatctgttga agaaacgtgg catcagcacc caggggctgg ttggcagctt
     6121 tgaaaaactg gctagtctgg atggcggtcg cacccagtcc atgtttgact ctcacccacc
     6181 atcaacagag cgtgcgcaac acatccgtga tcgtatcgcc tctggtaagt aaatcattgt
     6241 catctttcgg gctggtcttc tgccagcccg ctataattgc gcaataaatc cccatctgaa
     6301 tacagacaaa actggttttt gcacacaacg ttaacgattt gtggcgtcgg cgcgtataat
     6361 gcgcgcggtt atgttaacgg tacgcctgtt ttaaggagat aaagatgacg gcaattgccc
     6421 cggttattac cattgatggc ccaagcggtg cagggaaagg caccttgtgt aaggctatgg
     6481 cggaagcatt gcaatggcat ctgctggatt cgggtgcaat ttatcgcgta ctggcattgg
     6541 cggcattaca tcaccatgtt gatgttgcgt cagaagatgc gctggtaccg ctggcatccc
     6601 atctggatgt acgttttgtg tcaaccaatg gcaatctgga agtgatcctc gaaggggaag
     6661 atgtcagcgg cgaaattcgt actcaggaag tggcgaatgc agcttcacaa gtcgcggcat
     6721 tcccacgcgt tcgtgaagca ttattgcgtc gccaacgcgc gtttcgcgaa ttacccggcc
     6781 tgattgccga tggccgcgac atgggaacgg tggtattccc tgatgcgcca gtgaaaattt
     6841 tccttgacgc ctcctcggaa gaacgtgcgc atcgccgcat gctacagttg caggagaagg
     6901 gctttagtgt taactttgag cgccttttgg ccgagatcaa agaacgcgac gaccgcgatc
     6961 gtaaccgagc ggtagcgcca ctggttccgg cagccgatgc tttagtgttg gattccacca
     7021 ccttaagcat tgagcaagtg attgaaaaag cgctacaata cgcgcgccag aaattggctc
     7081 tcgcataagc gaccgaattt gcagtacccc cgttgcaatg gaatgacagc gggtatgtta
     7141 aacaacccca tccggcatgg agccaggtgg acgttaaata taaacctgaa gattaaacat
     7201 gactgaatct tttgctcaac tctttgaaga gtccttaaaa gaaatcgaaa cccgcccggg
     7261 ttctatcgtt cgtggcgttg ttgttgctat cgacaaagac gtagtactgg ttgacgctgg
     7321 tctgaaatct gagtccgcca tcccggctga gcagttcaaa aacgcccagg gcgagctgga
     7381 aatccaggta ggtgatgaag ttgacgttgc tctggacgca gtagaagacg gcttcggtga
     7441 aactctgctg tcccgtgaga aagctaaacg tcacgaagcc tggatcacgc tggaaaaagc
     7501 ttacgaagat gctgaaactg ttaccggtgt tatcaacggc aaagttaagg gcggcttcac
     7561 tgttgagctg aacggtattc gtgcgttcct gccaggttct ctggtagacg ttcgtccggt
     7621 gcgtgacact ctgcacctgg aaggcaaaga gcttgaattt aaagtaatca agctggatca
     7681 gaagcgcaac aacgttgttg tttctcgtcg tgccgttatc gaatccgaaa acagcgcaga
     7741 gcgcgatcag ctgctggaaa acctgcagga aggcatggaa gttaaaggta tcgttaagaa
     7801 cctcactgac tacggtgcat tcgttgatct gggcggcgtt gacggcctgc tgcacatcac
     7861 tgacatggcc tggaaacgcg ttaagcatcc gagcgaaatc gtcaacgtgg gcgacgaaat
     7921 cactgttaaa gtgctgaagt tcgaccgcga acgtacccgt gtatccctgg gcctgaaaca
     7981 gttgggcgaa gatccgtggg tagctatcgc taaacgttat ccggaaggta ccaaactgac
     8041 tggtcgcgtg accaacctga ccgactacgg ctgcttcgtt gaaatcgaag aaggcgttga
     8101 aggcctggta cacgtttccg aaatggattg gaccaacaaa aacatccacc cgtccaaagt
     8161 tgttaacgtt ggcgatgtag tggaagttat ggttctggat atcgacgaag aacgtcgtcg
     8221 tatctccctg ggtctgaaac agtgcaaagc taacccgtgg cagcagttcg cggaaaccca
     8281 caacaagggc gaccgtgttg aaggtaaaat caagtctatc actgacttcg gtatcttcat
     8341 cggcctggac ggcggcatcg acggcctggt tcacctgtct gacatctcct ggaacgttgc
     8401 aggcgaagaa gcagttcgtg aatacaaaaa aggcgacgaa atcgctgcag ttgttctgca
     8461 ggttgacgca gaacgtgaac gtatctccct gggcgttaaa cagctcgcag aagatccgtt
     8521 caacaactgg gttgctctga acaagaaagg cgctatcgta accggtaaag taactgcagt
     8581 tgacgctaaa ggcgcaaccg tagaactggc tgacggcgtt gaaggttacc tgcgtgcttc
     8641 tgaagcatcc cgtgaccgcg ttgaagacgc taccctggtt ctgagcgttg gcgacgaagt
     8701 tgaagctaaa ttcaccggcg ttgatcgtaa aaaccgcgca atcagcctgt ctgttcgtgc
     8761 gaaagacgaa gctgacgaga aagatgcaat cgcaactgtt aacaaacagg aagatgcaaa
     8821 cttctccaac aacgcaatgg ctgaagcttt caaagcagct aaaggcgagt aattctctga
     8881 ctcttcggga tttttattcc gaagtttgtt gagtttactt gacagattgc aggtttcgtc
     8941 ctgtaatcaa gcactaaggg cggctacggc cgcccttaat caatgcagca acagcagccg
     9001 cttaatttgc ctttaaggaa ccggaggaat catgaccaag tcagaattga tagaaagact
     9061 tgccacccag caatcgcaca ttcccgccaa gacggttgaa gatgcagtaa aagagatgct
     9121 ggagcatatg gcctcgactc ttgcgcaggg cgagcgtatt gaaatccgcg gtttcggcag
     9181 tttctctttg cactaccgcg caccacgtac cggacgtaat ccgaagactg gcgataaagt
     9241 agaactggaa ggaaaatacg ttcctcactt taaacctggt aaagaactgc gcgatcgcgc
     9301 caatatttac ggttaagttt ttactcaaac ttgaacgcga gaaaagcacc tgtcgggtgc
     9361 ttttttcatt tctctaatca ggaactggaa gctgcctcgc agagttttga gcagttttca
     9421 ccctttcgtt aaattcttct gaatgtgcct cggggaacgc aaaattccca ccagacaacc
     9481 gctcaacaaa gttgcacact ttccataaac agggaggggt gcatgaaaat aacgacagtc
     9541 ggcgtatgca taatttgcgg aatttttccg ttgctgattt tgccccaatt gcctgggaca
     9601 ttaacccttg cgtttctgac tctcttcgcc tgcgtactgg catttatccc tgttaaaacc
     9661 gtccgttata tcgcgctgac gttgctgttt ttcgtttggg gcatattatc agcaaagcaa
     9721 attttgtggg caggagaaac cttaactggc gcgacgcagg atgcaattgt tgagatcact
     9781 gcaactgacg gcatgaccac tcattacggt caaattactc atctacaagg tcgacgtata
     9841 ttccctgcgc caggccttgt gctgtatggc gaatatcttc cgcaagcggt ttgtgctgga
     9901 caacaatggt caatgaaact caaagttcgt gcagttcatg gccaacttaa tgatggcggc
     9961 tttgatagcc agcgttatgc cattgcccag catcagccgc tcaccggccg ctttctgcag
    10021 gcaagtgtca ttgaaccgaa ttgtagcctg cgtgcacagt atctggcgtc actacaaaca
    10081 acgctgcaac cctatatgtg gaatgcggtt attcttggtt taggtatggg ggaacggtta
    10141 tccgtcccca aagaaatcaa aaatatcatg catgatactg gaacggcgca tttaatggcg
    10201 atatcgggat tgcacatcgc ttttgcggcg ttgctggctg ccggactcat tcgcggtggg
    10261 caagtttatc tgcctgggcg ctggatccac tggcaaatgc cattaattgg tggaatctgc
    10321 tgtgctgctt tttatgcctg gttgacggga atgcaacctc ctgcattgcg taccgtggtg
    10381 gcgcttgcta cgtggggaat gcttaagtta agtgggcgac agtggagtgg ctgggatgta
    10441 tggatatgtt gtctggcggc aattttgctg atggatcctg ttgccattct ctcgcaaagt
    10501 ttatggctct ctgccgctgc ggtcgcggca ctgatttttt ggtaccagtg gtttccctgt
    10561 cctgagtggc aactgccgcc ggtattgcgt gcagttgttt ccctcattca tttgcaactg
    10621 ggaatcacac tcctgcttat gcctgtgcaa atcgtcatat ttcatggcat tagtctgacc
    10681 tcgtttattg caaatctatt agcaattccc ttggtgacat ttatcacggt tccgttgatc
    10741 ctcgccgcga tggttgtgca tttaagcggg ccgttaatcc tggagcaagg gttatggttt
    10801 cttgccgacc ggtctttggc tttacttttc tgggggttaa agagtttgcc agaagggtgg
    10861 atcaacattg ctgaacgttg gcaatggcta tcattttccc catggttctt actggtggta
    10921 tggcgattaa atgcctggcg aacgttgcca gcaatgtgtg tggctgtagg cttgctgatg
    10981 tgctggccgc tgtggcaaaa acctcgacct gacgagtggc aggtgtacat gcttgatgtc
    11041 gggcaagggc tggcaatggt gatagccaga aacggcaaag cgattctcta tgacacagga
    11101 ctggcctggc ccgaagggga tagtgggcaa caactgatta tcccctggct ccactggcat
    11161 aatcttgaac cggaaggcgt tattctgagt catgaacatc tggatcaccg gggagggctg
    11221 gactcaatat tgcacacatg gccgatgtta tggatcagaa gtccgttaaa ctgggaacat
    11281 catcagccct gtgtgcgtgg cgaagcgtgg caatggcaag gattgcgttt cagcgtgcac
    11341 tggcctttac aagctagcaa cgataaagga aataaccatt cctgtgtggt taaggttgat
    11401 gacgggacga atagcattct tctaaccggt gatattgaag tccccgctga acaaaagatg
    11461 ctaagccgtt actggcagca agtgcagaca acattgcttc aggtacctca ccatggcagt
    11521 aatacctcat catcgttgcc attaattcag cgagtgaatg gaaaagtggc actcgcatcg
    11581 gcatcgcgct ataacgcatg gcgattgccc tctaataaag ttaagcatcg ctatcaacag
    11641 caaggatatc aatggcttga tactccacat cagggtcaag tgacggtcaa tttttcagcg
    11701 caaggctggc ggattagcag cctcagagag caaattttac ctcgttggta tcatcagtgg
    11761 tttggcgtgc cagtggataa cgggtagaat atgcggctat ttcaacaaat gctggttttt
    11821 tgaatgcata acgacaaaga tctctctacg tggcagacgt tccgccgact gtggccaacc
    11881 attgcgcctt ttaaagcggg tctgatcgtg gcgggcgtag cgttaatcct caacgcagcc
    11941 agcgatacct tcatgttatc gctccttaag ccacttcttg atgatggctt tggtaaaaca
    12001 gatcgctccg tgctggtgtg gatgccgctg gtggtgatcg ggctgatgat tttacgtggt
    12061 atcaccagct atgtctccag ctactgtatc tcctgggtat caggaaaggt ggtaatgacc
    12121 atgcgtcgcc gcctgtttgg tcacatgatg ggaatgccgg tttcattttt tgacaaacag
    12181 tcaacgggga cgctgttgtc acgtatcacc tacgattccg aacaggttgc ttcttcttct
    12241 tccggcgcac tgataacggt tgtgcgtgaa ggtgcgtcaa tcatcggcct gttcatcatg
    12301 atgttctatt acagttggca actgtcgatc attttgattg tgctggcacc gattgtttcg
    12361 attgcgattc gcgtagtatc gaagcgtttt cgcaacatca gtaaaaacat gcagaacacc
    12421 atggggcagg tgaccaccag cgccgaacaa atgctgaagg gccacaaaga agtattgatt
    12481 ttcggtggtc aggaagtgga aacgaaacgc tttgataaag tcagcaaccg aatgcgtctt
    12541 caggggatga aaatggtttc agcctcttcc atctctgatc cgatcattca gctgatcgcc
    12601 tctttggcgc tggcgtttgt tctgtatgcg gcgagcttcc caagtgtcat ggatagcctg
    12661 actgccggta cgattaccgt tgttttctct tcaatgattg cactgatgcg tccgctgaaa
    12721 tcactgacca acgttaacgc ccagttccag cgcggtatgg cggcttgtca gacgctgttt
    12781 accattctgg acagtgagca ggagaaagac gaaggtaagc gcgtgatcga gcgtgcgact
    12841 ggcgacgtgg aattccgcaa tgtcaccttt acttatccgg gacgtgacgt acctgcattg
    12901 cgtaacatca acctgaaaat tccggcaggg aagacggttg ctctggttgg acgctctggt
    12961 tcaggtaaat caaccatcgc cagcctgatc actcgttttt acgatattga tgaaggcgaa
    13021 atcctgatgg atggtcacga tctgcgcgag tataccctgg cgtcgttacg taaccaggtt
    13081 gctctggtgt cgcagaatgt ccatctgttt aacgatacgg ttgctaacaa cattgcttac
    13141 gcacggactg aacagtacag ccgtgagcaa attgaagaag cggcgcgtat ggcctacgcc
    13201 atggacttca tcaataagat ggataacggt ctcgatacag tgattggtga aaacggcgtg
    13261 ctgctctctg gcggtcagcg tcagcgtatt gctatcgctc gagccttgct gcgtgatagc
    13321 ccgattctga ttctggatga agctacctcg gctctggata ccgaatccga acgtgcgatt
    13381 caggcggcac tggatgagtt gcagaaaaac cgtacctctc tggtgattgc ccaccgcttg
    13441 tctaccattg aaaaggcaga cgaaatcgtg gtcgtcgagg atggtgtcat tgtggaacgc
    13501 ggtacgcata acgatttgct tgagcaccgc ggcgtttacg cgcaacttca caaaatgcag
    13561 tttggccaat gatcgaaaaa atctggtctg gtgaatcccc tttgtggcgg ctattgctgc
    13621 cactctcctg gttgtatggc ctggtaagtg gcgcgatccg tctttgctat aaactaaaac
    13681 tgaagcgcgc ctggcgtgcc cccgtaccgg ttgtcgtggt tggtaatctc accgcaggcg
    13741 gcaacggaaa aaccccggtc gttgtctggc tggtggaaca gttgcaacag cgcggtattc
    13801 gcgtgggggt cgtatcgcgg ggatatggtg gtaaggctga atcttatccg ctgttattgt
    13861 cggcagatac cacaacagca caggcgggtg atgaacctgt gttgatttat caacgcactg
    13921 atgcgcctgt tgcggtttct cccgttcgtt ctgatgcggt aaaagccatt ctggcgcaac
    13981 accctgatgt gcagatcatc gtaaccgacg acggtttaca gcattaccgt ctggcgcgtg
    14041 atgtggaaat tgtcgttatt gatggtgtgc gtcgctttgg caatggctgg tggttgccgg
    14101 cggggccaat gcgtgagcga gcggggcgct taaagtcggt tgatgcggta atcgtcaacg
    14161 gcggtgtccc tcgcagcggt gaaatcccca tgcatctgct gccgggtcag gcggtgaatt
    14221 tacgtaccgg tacgcgttgt gacgttgctc agcttgaaca tgtagtggcg atggcgggga
    14281 ttgggcatcc gccgcgcttt tttgccacgc tgaagatgtg tggcgtacaa ccggaaaaat
    14341 gtgtaccgct ggccgatcat cagtctttga accatgcgga tgtcagtgcg ttggtaagcg
    14401 ccgggcaaac gctggtaatg actgaaaaag atgcggtgaa atgccgggcc tttgcagaag
    14461 aaaattggtg gtatttgcct gtagacgcac agctttcagg tgatgaacca gcgaaactgc
    14521 ttacgcaact aacctcgctg gcttctggca actagttacg ccgcggcagc gttcgattga
    14581 tggagtcatg aatgtcgctg ccgcacctct cccttgctga tgcgcgtaat cttcaccttg
    14641 ccgcacaagg cctgttaaac aaaccccgcc gtcaagcgtc gttggaggat attccggcaa
    14701 cgatctcccg catgtccttg ctgcaaatcg ataccatcaa tattgttgcc cgtagtccat
    14761 atctggtgct tttcagtcgt ctgggaaatt atcctgccca gtggctggat gagtctctgg
    14821 cgcgtggcga attaatggaa tactgggcgc atgaagcctg ctttatgccg cgtagcgact
    14881 ttcgtcttat tcgccaccgc atgctggcac ctgaaaaaat gggctggaaa tacaaagacg
    14941 cctggatgca ggaacatgag gcggaaattg cacagttaat tcagcatatt catgataagg
    15001 ggccggtacg ttcagccgat tttgagcatc ctcgtaaagg tgcaagcggc tggtgggaat
    15061 ggaagccgca taaacggcat ctggaaggtt tatttactgc cggaaaggtg atggtgattg
    15121 aacggcgcaa cttccagcgc gtttatgatt taacccaccg tgtcatgcct gactgggatg
    15181 atgagcgcga tctcgtttcg caaacagaag cagaaatcat catgctggat aacagtgcgc
    15241 gtagcctggg aatattccgc gaacagtggc tggcagatta ctatcggctg aaacgtccgg
    15301 cactggcggc gtggcgcgaa gcgagggctg aacagcagca aatcattgct gtgcatgttg
    15361 aaaaattggg caatctttgg ctgcatgctg atttgctgcc gctactcgag cgtgcgctgg
    15421 ccggaaagct cactgcaacg cacagcgcgg tactttcgcc ttttgatcct gttgtctggg
    15481 atcgcaaacg cgcagagcag ctttttgatt ttagctaccg gctggagtgc tataccccag
    15541 cgccgaaacg ccagtatggc tattttgttc tgccattatt acatcgtggg caattagttg
    15601 ggcgaatgga tgccaaaatg catcgccaga caggcatcct tgaagttatc tctctgtggt
    15661 tacaggaagg cattaaacca acgacaatgc tgcaaaaagg gttacgtcag gcgattactg
    15721 atttcgcgag ctggcagcag gcaacgcggg tgacattagg acgctgcccg caaggtctct
    15781 ttactgattg ccgcactggc tgggaaatag accccgtcgc ataaatgatt atgataagat
    15841 ttatgcattc ataacccgat ccatctggag gaactatgga tcatcgtttg cttgaaatca
    15901 ttgcctgccc ggtttgcaac ggaaaacttt ggtataacca ggaaaaacaa gagcttattt
    15961 gcaaactgga taacctggct ttcccgctcc gcgatggcat tccagtgttg ctggaaacgg
    16021 aagcccgcgt gctgactgct gatgagagta aatcatgagt tttgtggtca ttattcccgc
    16081 gcgctacgcg tccacgcgtc tgcccggtaa accattggtt gatattaacg gcaaacccat
    16141 gattgttcat gttcttgaac gcgcgcgtga atcaggtgcc gagcgcatca tcgtggcaac
    16201 cgatcatgag gatgttgccc gcgccgttga agccgctggc ggtgaagtat gtatgacgcg
    16261 cgccgatcat cagtcaggaa cagaacgtct ggcggaagtt gtcgaaaaat gcgcattcag
    16321 cgacgacacg gtgatcgtta atgtgcaggg tgatgaaccg atgatccctg cgacaatcat
    16381 tcgtcaggtt gctgataacc tcgctcagcg tcaggtgggt atggcgactc tggcggtgcc
    16441 aatccacaat gcggaagaag cgtttaaccc gaatgcggtg aaagtggttc tcgacgctga
    16501 agggtatgca ctgtacttct cacgcgccac cattccttgg gatcgtgatc gttttgcaga
    16561 aggccttgaa accgttggcg ataacttcct gcgtcatctt ggtatttatg gctaccgtgc
    16621 aggctttatc cgtcgttacg tcaactggca ggcaagtccg ttagaacaca tcgaaatgtt
    16681 agagcagctt cgtgttctgt ggtacggcga aaaaatccat gttgctgttg ctcaggaagt
    16741 tcctggcaca ggtgtggata cccctgaaga tcttgagcgc gttcgcgctg aaatgcgcta
    16801 atttcacttc acgacacttc agccaatttt gggaggagtg tcgtaccgtt acgattttcc
    16861 tcaatttttc ttttcaacaa ttgatctcat tcaggtgaca tcttttatat tggcgctcat
    16921 tatgaaagca gtagctttta tgagggtaat ctgaatggaa cagctgcgtg ccgaattaag
    16981 ccatttactg ggcgaaaaac tcagtcgtat tgagtgcgtc aatgaaaaag cggatacggc
    17041 gttgtgggct ttgtatgaca gccagggaaa cccaatgccg ttaatggcaa gaagctttag
    17101 tacgcccgga aaagcccgac aactggcatg gaaaaccacc atgctggcaa gaagtgggac
    17161 tgtccgtatg ccgactattt atggtgtgat gacgcatgaa gaacaccccg gccctgatgt
    17221 cctgctactg gagcggatgc gtggtgtttc ggtggaggca ccagcccgaa caccagaacg
    17281 ctgggaacag ctcaaagacc aaatcgttga agccttactg gcctggcacc gtcaggacag
    17341 tcgcggttgc gtcggcgcgg tcgacaatac tcaggaaaat ttctggccct catggtacct
    17401 gcaacatgtt gaagtgctat ggaccacgct caatcagttc aataacaccg gtctgacgat
    17461 gcaggataag cggatcctgt ttcgcactcg cgaatgtctc ccggcattat ttgaaggctt
    17521 taacgacaat tgtgtgttga ttcacggtaa cttctgttta cgcagcatgc tgaaagattc
    17581 gcgcagcgat cagttactgg cgatggtcgg gccgggacta atgctttggg caccgcgaga
    17641 atacgaactg ttccgactaa tggataattc tctggcggaa gatttgctct ggagttacct
    17701 gcaacgcgcg ccagtggcgg agtcgttcat ctggcggcgt tggttgtatg tgttatggga
    17761 tgaagttgcg caactggtta ataccggacg atttagtcgg cgcaacttcg atctggcatc
    17821 aaaatcactc ttgccgtggc tcgcctgacg gacctttcag ccactgccag atgcggccaa
    17881 gcgtttcata gccaacacga tcgctatgca tcagccagac cggagaaggg attgcccgtt
    17941 cccacgggtt aagcggcgag tcgatggcca actgattcgc cggggctggt agcggattta
    18001 acccttcctg ctgaaaaaag atcatcgccc gcggcagatg tgatgcggag gtcaccagca
    18061 ggaaaggggc gtcaccaatc gcctgtttca ctgccgcagc ttcttcttcg gtatcttttg
    18121 gcaaatccag ggtgataatt tgctcgcgcg gcacgcccag cgattgcgca actctggctc
    18181 ctacttccgc tgtactcacc gtattggttt ttgctacgcc tcccgtgaag atcagttttg
    18241 atcccggatt ttcgcgccat aagcgaatac cttcattcag gcgtggcaga ctgttattga
    18301 ttaaattaga gctcggtgcc cactgcgggt tccaggtata accacctccg agcaccacga
    18361 tatagtccac tttttgcgaa ttattccatg tcggataggt gctttcgatg ggacgcagca
    18421 ggcggtcggc taccggttgc aaactcaata acaagagtgc cagccaccca atgctaataa
    18481 aaatcttccc ggttttttga aaacggctaa accacagcag cgccaggcca gcgccaataa
    18541 ttaacaacat cagcggaagt ggcagcaaca tattgccaat cacttttttc agtgtaaaaa
    18601 gcattctttc tggttccttt tttaaccata tagcaaggga tcgccagcga tattacacca
    18661 gacgggttca ttctcgccgt ggctgtgaca aaatagcgtc tttatcagaa aacgtcttag
    18721 ccaaccggtg gagaaagcaa tgcaggatcg caattttgat gatattgcgg aaaagttttc
    18781 ccgtaacatt tacggcacca ccaaagggca gcttcgacag gctattctgt ggcaggatct
    18841 cgatcgcgtg ctggcggaaa tgggcccgca aaaactgcgt gtgctggatg ctggcggtgg
    18901 agaagggcag accgcaatca aaatggccga gcgtgggcat caggtcattt tatgcgatct
    18961 ttctgcgcag atgatcgacc gcgcaaaaca ggcggcagaa gcaaaaggtg tgagcgacaa
    19021 catgcaattt atacattgcg ccgctcagga tgttgcttcg catttggaaa cgcccgttga
    19081 tctgatattg ttccatgcgg tgctcgagtg ggtggccgat ccccgcagtg tattgcagac
    19141 cctgtggtca gtgttgcgtc caggcggcgt gttgtcgtta atgttctaca atgcgcatgg
    19201 tttgttgatg cataacatgg tcgccgggaa ttttgattac gtgcaggcgg gaatgccgaa
    19261 aaagaaaaaa cggacgcttt cgccagatta tccacgcgac ccggcgcagg tttatctgtg
    19321 gctggaagaa gctggttggc aaattatggg taagacaggc gttcgcgtgt ttcatgatta
    19381 tctgcgcgag aagcaccagc agcgcgactg ctatgaagca ttacttgaat tagaaacgcg
    19441 ttattgccgt caggaaccgt atattaccct ggggcgttat attcatgtca ccgcgcgcaa
    19501 accgcagagc aaggataaag tatgagtgaa ttttcccaga cagtccccga actggttgcc
    19561 tgggccagaa aaaatgactt ctccatctcg ctgccggtag accgactctc ttttctgctg
    19621 gcggttgcca cgctgaacgg cgagcgtctg gatggtgaga tgagtgaagg cgagctggtg
    19681 gatgcattcc gccatgtgag tgatgcgttt gagcaaacca gcgaaaccat cggcgtgcgc
    19741 gccaataacg cgatcaacga catggtgcgt caacgtctgc tgaaccgctt taccagcgag
    19801 caggcggaag ggaacgcaat ttaccgtctg acgccgctcg gcatcggcat tactgactac
    19861 tacatccgtc agcgcgagtt ttctacgctg cgtctttcta tgcagttgtc gattgtggcg
    19921 ggtgagctca aacgcgcagc agatgccgcc gaagagggcg gtgatgaatt tcactggcac
    19981 cgtaatgtct atgcgccact gaaatattcg gtagcagaaa ttttcgacag tatcgacctg
    20041 acgcaacgtc tgatggacga acagcagcag caggtgaagg acgatatcgc ccagttgctg
    20101 aacaaagact ggcgggcggc gatttccagc tgtgaattgt tgctttcgga aacttccggg
    20161 acgctgcgtg aattgcagga tacgctggaa gcggcaggcg acaaattgca ggctaatctg
    20221 ttgcgcattc aggatgcgac gatgacccat gacgatctgc atttcgtcga tcgtctggtg
    20281 ttcgatctgc agagcaaact cgatcgtatt atcagttggg gccagcaatc catcgacttg
    20341 tggattggct acgaccgcca cgtacacaaa tttattcgta ccgcgatcga tatggataaa
    20401 aaccgcgtct ttgctcagcg gttacgtcag tcggtacaaa cctattttga tgagccgtgg
    20461 gcgctaactt atgccaatgc cgatcgtctg ctggatatgc gtgacgaaga gatggcactg
    20521 cgcgatgaag aagtgactgg ggaacttcct gaggatctgg aatacgaaga gtttaacgag
    20581 atccgcgaac agctggcggc gatcatcgaa gaacaacttg ccgtgtacaa aaccagacaa
    20641 gtgccgctgg atcttggtct ggtggtacgc gaatatctgt cacagtatcc gcgtgcacgt
    20701 cactttgacg ttgcgcgtat tgttattgat caggcggtac gtcttggcgt agcgcaagca
    20761 gatttcaccg gactgccagc gaaatggcag ccgattaatg attacggagc caaggtacag
    20821 gcgcatgtca tcgacaaata ttgaacaagt gatgccggtt aagctggcgc aggcgctggc
    20881 gaatccgtta tttccggcgc tggacagcgc cttacgttca ggccgccata ttggcctcga
    20941 cgaactggat aatcatgcat tcctgatgga ttttcaggaa tatctggaag agttttacgc
    21001 gcgttataac gttgagctta ttcgcgcacc agaagggttc ttctatttac gcccacgttc
    21061 caccacgctg atccctcgtt ccgtcttgtc ggaactggat atgatggtcg ggaaaatcct
    21121 ctgttatctc tatctcagcc cggaacggct ggcgaatgag gggattttca cccagcagga
    21181 actgtacgac gaactgctca ccctggccga tgaagcaaaa ctgctgaaac tggtgaacaa
    21241 ccgttcaacc ggttcagacg ttgaccgtca gaagttgcag gagaaagtac gttcttcgct
    21301 caaccgtctg cgtcgtttag gcatggtgtg gtttatgggc cacgacagca gcaagtttcg
    21361 cattaccgaa tcggtgttcc gcttcggagc cgatgtgcgt gctggcgacg atccccgtga
    21421 agcacagcgt cgcctgattc gtgatggcga agcaatgccg attgaaaatc atctgcaact
    21481 caacgatgaa accgaagaaa atcagccaga tagcggagag gaagaataat gattgaacgc
    21541 ggtaaatttc gctcactgac gctgattaac tggaacggct tttttgcccg aacttttgac
    21601 cttgacgagc tggtcacgac gctttctggc ggtaacgggg cgggtaaatc caccaccatg
    21661 gcggcgttcg ttacggcgct gatccccgac ctgaccctgc tgcatttccg taacactacg
    21721 gaagccgggg ccaccagcgg ttcgcgcgat aaaggtctgc acggtaagct gaaagcgggt
    21781 gtctgttatt cgatgctcga caccattaac tcgcgccacc agcgcgtggt ggtcggtgtg
    21841 cgtctgcaac aggttgccgg acgcgatcgt aaagtggata tcaaaccatt tgccattcag
    21901 ggactgccga tgtcggtgca gccgacacag ctggtgaccg aaaccctgaa cgaacgccag
    21961 gcgcgcgtgc tgccgcttaa cgagctgaaa gacaagctcg aggcgatgga aggcgtgcag
    22021 tttaaacagt tcaactccat taccgattac cactcgctga tgttcgatct gggcatcatc
    22081 gcgcgtcgtc tgcgctccgc atctgaccgt agcaaattct atcgtctgat cgaagcttcg
    22141 ctgtatggcg ggatctccag tgccattacc cgttctctgc gcgactacct gttgccggaa
    22201 aacagcggcg tgcgtaaagc gttccaggac atggaagcgg cgctgcgtga aaaccgcatg
    22261 acgctggaag cgattcgtgt cacccagtcg gaccgtgacc tgtttaagca tctgatcagc
    22321 gaagctacca actacgtggc ggcggactac atgcgtcacg ccaacgagcg ccgtgtccat
    22381 ctcgacaaag ccctggagtt tcgtcgcgag ttacatactt cgcgtcagca actggcggct
    22441 gagcagtaca aacacgtcga tatggcgcgt gagctggcag agcacaacgg tgccgaaggc
    22501 gatctggaag cggattatca ggcggccagc gatcacctga acctggtgca aaccgcactg
    22561 cgtcagcagg agaagatcga acgctacgaa gcggatctcg atgagctgca gatccgtctg
    22621 gaagagcaaa acgaagtggt ggcagaagcc atcgaacgcc aggaagagaa cgaggctcgc
    22681 gcggaagctg ccgaactgga agtggacgag ctgaaaagcc agcttgctaa ctaccagcag
    22741 gcgctggacg tccagcaaac gcgcgcgatc cagtataacc aggcgattgc tgcgcttaat
    22801 cgcgccaaag aactgtgcca tctgccggac ttaaccgccg acagcgccgc cgaatggctg
    22861 gaaaccttcc aggcgaaaga gctggaagcg actgaaaaaa tgctctctct tgagcagaaa
    22921 atgagcatgg cgcaaaccgc gcacagccag tttgagcagg cttatcagct ggtggtggca
    22981 atcaacggcc cgctggcgcg taacgaggcg tgggatgtcg ctcgcgaact attgcgcgaa
    23041 ggggtcgatc agcgtcacct ggcagagcag gttcagccgt tgcggatgcg cttaagcgaa
    23101 ctggaacagc gtctgcgcga acagcaagaa gctgagcgtc tgctggcaga tttctgcaaa
    23161 cgtcagggca agaattttga cattgacgag ctggaagctc tgcatcagga gctggaagcg
    23221 cgcattgcct ctctttccga tagcgtgtct aacgcccgtg aagagcgcat ggcactgcgc
    23281 caggaacagg aacagcagca gtctcgcatt cagagtttga tgcagcgtgc gccggtttgg
    23341 ctggcagcgc aaaacagtct caaccagttg agcgaacagt gcggcgaaga gtttacctcc
    23401 agccaggacg tcaccgaata tctgcaacag ttgctggagc gtgagcgaga ggcgattgtt
    23461 gaacgcgatg aagtgggcgc gcgcaaaaac gccgtcgatg aagagatcga acgtttaagc
    23521 cagcctggcg gctctgaaga tcagcgtctg aacgcgctgg cggagcgttt tggtggtgtg
    23581 ctgctgtcag aaatttatga cgacgttagc ctggaagatg cgccgtactt ctcagcgctg
    23641 tatggcccgt cacgccacgc catcgtggtg ccagatctgt cacagataac tgaacacctg
    23701 gaaggcttga ccgattgccc ggaagatctc tatctgatcg aaggagatcc gcagtcattc
    23761 gatgacagcg tgttcagcgt tgatgagctg gaaaaagcgg tagtggtgaa aatcgccgat
    23821 cgtcagtggc gttattcacg tttcccggaa gtgccgctgt ttggtcgtgc tgcgcgtgaa
    23881 agccgtattg aaagcctcca tgccgagcgt gaagtgcttt ccgaacgctt cgccacgctc
    23941 tcctttgatg tacagaaaac tcagcgtctg catcaggcgt tcagccgctt tatcggcagt
    24001 catctggcgg ttgcgtttga gtctgacccg gaagcagaaa tccgtcaact gaacagccgt
    24061 cgcgtcgaac tggagcgggc gttgagtaat catgaaaatg ataaccagca gcagcgtatt
    24121 cagtttgagc aggcgaaaga aggcgttacg acgctgaacc gcattctgcc gcgtctcaac
    24181 ctgttggctg atgacagcct ggcggatcgc gtcgatgaaa tccgcgaacg tctggatgaa
    24241 gcccaggaag ccgcgcgttt tgttcagcag tttggcaatc aactggcgaa actggaaccg
    24301 atcgtttcgg tattgcagag cgacccggaa cagttcgaac agctaaaaga agattacgcg
    24361 tactctcagc agatgcagcg cgatgcccgt cagcaggcgt ttgccctgac ggaagtggtg
    24421 cagcgtcgtg cgcactttag ctattctgac tcggcagaaa tgctgagcgg taatagcgat
    24481 ctcaacgaaa agctgcgtga acgtctggaa caggcggaag cggagcgtac tcgcgctcgt
    24541 gaagcgttgc gtggtcacgc agcgcagttg agtcagtaca accaggtgct ggcttcgctg
    24601 aaaagttctt acgacaccaa aaaagagcta ctcaacgatc tgcaacgtga attgcaggat
    24661 atcggcgtgc gtgctgatag cggggcagaa gagcgggcgc gtattcgccg tgacgagctg
    24721 catgcgcaac tgagcaataa ccgttcacgc cgcaatcaac tggaaaaagc gcttaccttc
    24781 tgcgaagcgg agatggacaa cctgacccgc aaactgcgca agctggagcg ggattacttt
    24841 gagatgcgcg agcaggtagt gaccgccaaa gcgggctggt gtgcggtgat gcgcatggtg
    24901 aaagataacg gcgttgagcg ccgcttacac cgtcgtgagc tggcttatct ctccgctgat
    24961 gatttgcgct ctatgtcgga taaggcattg ggtgcgctgc gtctggcggt ggcggataac
    25021 gaacatctgc gcgacgtgct gcgcatgtcg gaagatccga aacgtccgga gcgtaaaatt
    25081 cagttcttcg tggcggttta tcagcatctg cgtgaacgta ttcgtcagga tattattcgt
    25141 accgatgatc cggtggaagc tatcgaacag atggagatcg aacttagccg tctgaccgaa
    25201 gaattaacct cccgtgaaca gaaactggcg atcagttccc gcagcgtggc gaacatcatt
    25261 cgcaaaacca ttcagcgcga gcagaaccgt atccgtatgc tcaaccaggg gttgcagaac
    25321 gtatcgtttg gtcaggtgaa cagcgtgcgc ctcaacgtga acgtgcgtga aacgcacgcc
    25381 atgctgctgg atgtgctctc tgaacagcac gagcagcatc aggatctgtt taacagcaac
    25441 cgtctgacct tctcggaagc gttggcgaaa ttgtatcagc gtcttaatcc gcagattgat
    25501 atgggacagc gcacgccgca gaccattggc gaagaactgc tggattaccg caactatctg
    25561 gaaatggaag ttgaggttaa ccgtggttcc gatggttggt tgcgcgcaga gtctggtgca
    25621 ttgtcgaccg gtgaggcgat tggtaccggt atgtcgattc tggtgatggt ggtacaaagc
    25681 tgggaagatg aatctcgccg cctgcgcggt aaagatatct ctccttgccg cctgctgttc
    25741 ctcgatgaag cagcgcgact ggatgctcgt tctatcgcca cgctgtttga attgtgtgag
    25801 cgtttgcaaa tgcagctcat catcgcagcg ccggaaaata tcagcccgga gaaaggcacc
    25861 acctataaac tggtgcgtaa agtcttccag aataccgaac acgttcacgt cgtcggcctg
    25921 cgaggatttg cgccgcaact ccctgaaacg cttccaggaa gtgacgaagc gccttctcag
    25981 gcgagttaaa attaaggcgg cagcaatgcc gccttttctt tttccgaaaa ctccgtttcc
    26041 gcactaaaaa agtggcacat tacggcgcgc ttttctttaa acttctttac attaggttat
    26101 gtaaaaacgt atcggcgttt atatactgaa gataagcctg atgagtaaca ggcttgctcg
    26161 tcatactttc gtgagtattg gcgttgtaca ggcaagtcgt aaaataacag cctggctatt
    26221 cagagtatga taaaaacagg gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc
    26281 tgtcggcaat cagtttgtgc ctggccgtaa cattcgctcc actgttcaat gcgcaggccg
    26341 atgagcctga agtaatccct ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac
    26401 tgccgcaggc gcaagccacg gcaataatgg cggggatcca gccattgcct gaaggtgcgg
    26461 cagaaaaagc ccgcacgcaa atcgaatctc aattacccgc aggttacaag ccggtttatc
    26521 ttaaccagct tcaactgttg tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg
    26581 atgctgttaa agccttccag caacagctgg cagaggtggc gattgccggt ttccagccgc
    26641 agtttaataa atgggtagag ttactgaccg atcctggtgt taacgggatg gcacgcgacg
    26701 tagtgctctc tgatgcgatg atgggctatc tccatttcat tgcaaatatt ccggtcaaag
    26761 gcactcgctg gctatatagc agtaaacctt atgcacttgc aacgccgccg ctctcggtga
    26821 ttaaccaatg gcagctggcg ctggataaag gtcaattgcc tacgtttgtt gcaggactgg
    26881 caccgcagca tccgcaatat gcggcgatgc atgaatcgtt actggcctta ctcagtgaca
    26941 ccaaaccgtg gccccaactg accggcaaag caacgttgcg cccagggcag tggagtaacg
    27001 acgtaccggc gttgcgcgaa atattgcaac gcacaggcat gttggacggg gggccgaaaa
    27061 ttactctacc tggcgatgac acgccaactg acgcggtagt cagcccatcc gctgttactg
    27121 ttgaaacagc agaaactaag ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc
    27181 ctgccgttcg tgccgcctac gataatgaac tggtggaagc cgttaaacgt tttcaggcat
    27241 ggcaaggatt gggggcagat ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa
    27301 cgcccgccca gcgtgctggc gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa
    27361 cagagctttc taccgggatc atggttaaca ttccggccta ttcgctggtc tactataaga
    27421 acggcaatca ggtgctggat tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga
    27481 tgatgagcag tgcccttaac aacgtagtgg taaacccgcc gtggaacgta cctccaactc
    27541 tggcacgcaa agatattctg ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg
    27601 gctatacggt gatgcgcggc tggaacagca gagaagcgat tgacccatgg caggttgact
    27661 ggtctacaat cacggcctcg aatttaccgt tccgcttcca gcaggctcca ggcccacgga
    27721 actcgctggg gcgctataaa ttcaatatgc cgagttcaga ggccatttat ttgcatgaca
    27781 cgccgaacca caatctgttc aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag
    27841 tgaataaagc ttccgatctg gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac
    27901 gtatttctga tgcgctgaag cagggtgata cacgttacgt caatattcgg cagtcgattc
    27961 cggtgaatct ctactacctg acggcctttg ttggtgcaga tggtcgtacc cagtatcgta
    28021 cagatattta caattatgat ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg
    28081 aacaattaat caggtaaatg aagtagttcg gggaaatcag ttgtcgtaat aattagcatg
    28141 attgggggcg attctctgca gcccccgtca ctgctggggt tgagtcatct tgacgtctgc
    28201 tttacgggcg gttaaggtgc ctcttgtgcg ccagaagtgc atataaacga taacattgac
    28261 ctgtagactt gattatcatg gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg
    28321 gtggcgttgc actcggtgcc gccatcctgc cgacccctgc gtttgcaaca ctctctaccc
    28381 cacgcccgcg cattttgaca ctcaataatc ttcataccgg agagtcaatc aaagcggagt
    28441 ttttcgatgg cagaggctat attcaggaag aattggcaaa acttaaccat tttttccgcg
    28501 attaccgcgc gaacaaaata aagtccatcg acccaggatt attcgaccag ttgtatcgcc
    28561 tgcaagggtt gttaggcacg cgcaaaccgg tgcaactcat ttccggttat cgttctattg
    28621 ataccaacaa tgaactacgc gcccgcagcc gtggagtagc gaagaaaagc tatcacacta
    28681 aaggccaggc gatggatttc catattgaag gtatcgcgtt aagcaatatt cgcaaagccg
    28741 cgttatctat gcgcgcaggt ggtgtaggat attatccacg tagtaacttt gtgcatattg
    28801 ataccgggcc agcacggcac tggtagtaat tgcttaacga aacaggggca gtatgaacta
    28861 tcgtattatt ccggtcaccg cattctccca gaactgttca ttaatctggt gtgaacaaac
    28921 ccgtctggcc gcactggtcg atcctggcgg cgatgcggaa aaaatcaaac aggaagttga
    28981 tgacagcggc ctgacactga tgcagatcct gctgacgcat ggtcatctgg accacgttgg
    29041 cgcagcggcg gaactggcgc aacattacgg cgtgccggtt tttggcccgg aaaaagaaga
    29101 tgagttctgg ctgcaaggct tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca
    29161 gccgctgacg ccagatcgtt ggctgaacga aggcgatacc atcagcatag ggaatgtgac
    29221 tttacaggtg ttacattgcc ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg
    29281 ggcaaagctg ctgatttctg gcgatgttat tttcaaaggc ggagtagggc gcagtgactt
    29341 cccgcgtggc gatcataatc aactgatttc ttcaatcaaa gataaattgc tgccactggg
    29401 ggatgacgtg acatttattc cgggtcacgg accattatcc acacttggtt atgaacgcct
    29461 gcataatccc ttcctgcaag acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct
    29521 tttaatgcgg gcctggattt ctggcaaagt gcgctttgtt tatgccggat gcggcacgag
    29581 cgccttatcc ggcctacaaa atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct
    29641 gataagcgta gcgcatcagg caatgttgcg tttgtcatca gtctcagccc gcttttcagc
    29701 gggcttcatt gtttttaatg cttacagcac tgccacaatc gcttcgcaca gcggagccat
    29761 gttatctggt gtcatcccgg ccacatttac gcgaccagaa gcaaccgcat atacgccaaa
    29821 ctcttcgcgc agacgcagca cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt
    29881 ctgtttgatg ataaagctga agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa
    29941 caactgacgc atacgctgaa tacgctggcg catatcagtc agctcttgtt cccaaatcgc
    30001 acgtaacgca tcgttgctca ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt
    30061 agagtagtta gcgcgaatcg ccgctttcat ttggctgaat gcgcgatcaa cggtttcact
    30121 gtcggcagca accagagtac aagcgccaac acgctcgttg tacaggccaa agtttttaga
    30181 gtaggaactg gcaacaatca gctctttatg catagccgcg aaagcgcgca gtccttcagc
    30241 atcttcttcc agaccacggg caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc
    30301 tttctcaact gagagttgtg ccagtgtctg ccattgttcc agcgtagggt cgataccggt
    30361 tgggttatgg cagcagccat ggaacagcac tacgtcgcca gcctgagctt cgttcaggct
    30421 gttaatcagt gcatcgaagt caagggtgtg gttttctgca tcataataag cgtattcacg
    30481 aacttccaga cctgcagagt taaagacgct cttatggttc ggccagcttg ggttgctcac
    30541 ccacactcgc ttaacgctgg tattttttgc caggaaatcg gcagctacgc gtagtgcgcc
    30601 agtgcccccc ggagtctgtg ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt
    30661 accaaacagc agttcctgag tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta
    30721 atttttggtg gtttcatttt cgagcagata ctgttcagcc tttttcacgc tggtcaatac
    30781 cggggttttg cccgtctcat ctttatagac accaatcccg aggttaattt tgccgggacg
    30841 ttcatcggca cgaaacagat cggccaggcc cagaatcggg tcggcaggag cggcggtaat
    30901 gttctcaaac atgacgaggt tccattatgg ttacagaagg gaagtccgct atcagggtaa
    30961 cgggagattt acaaaattcc aactattact gatgaaaacg caggctgttt ttgcaagacg
    31021 tgagattgct ctggaaggca taaaaaaaac aggaccaaag tcctgttttt tcggcattta
    31081 acaaagaggt gtgctattag aactggtaaa cgatacccac agcaacggtg tcgtcagaac
    31141 ctacgcccag tttgttgtca gaatcgatct ggttgatgat gtagtcaaca taggtggaca
    31201 tgtttttgtt gaagtagtag gttgcgccca cttcaaagta gttcaccaga tcaacatcac
    31261 cgataccttc tacgtctttc gctttagatt tggtgtaagc gatggacgga cgcagaccga
    31321 aatcgaactg gtattgcgca actaacagaa cgtcttgcgt tttgttggcg aagccgctgg
    31381 tgtttgtaaa tttattagtg atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca
    31441 ggtagatgtt gttcgcgtcg tacttcagac cagtagccca ctgttcagct tttttaccgt
    31501 tgccaagagg ttgagcttct tgcaggttgg tacggtcagc tgcaccataa gcaccaacga
    31561 taccaaagcc ttcgtattcg tagctgatag aaccgccaac accgtcgccg ttagaacggc
    31621 gtgcagtgtc acgctcgttt ttacccaggt actgaacagc gaagttcagg ccatcaacca
    31681 gaccaaagaa gttggagtta cgataggtag caacgccgcc aacacgacca acgaagaagt
    31741 catcgctgta tgcagtatca ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat
    31801 cataaaccac accgtagtta cggccgtaat cgaaagaacc aacgtcagcg tatttaagac
    31861 ccgcgaatgc cagacgcgtt ttgttaccag tttgagcgtc agcgccttca gagttgttac
    31921 cctggaagtt atattcccac tgaccataac cggtcagatc ggaattgatt tgagtttccc
    31981 ctttaaaacc aagacgggca taggtcatgt cgccattgcc accgtaactg ttttcaccgt
    32041 tacccttgga aaaataatgc agaccaacag ctttaccgta cagatctact ttgttgccat
    32101 ctttgttata gatttctgca gcgtttgcag tacctgctac taacagagca gggacgatca
    32161 ctgccagaat attgcgcttc atcattattt attaccctca tggttttttt tatgacacct
    32221 gccactgccg tcaataagtt ctgtcaataa aaatttacgg aactattgat gagagtttgg
    32281 tgtctttatg tgtctgcagg catctttcca ttcaaactaa cgtttcgcta ccgtgaaagt
    32341 gctacaaaga taaagattta gtttcaaaaa gaaaaaatat gtaa
//
LOCUS       DAZGTR010000043        32300 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGTR010000043 DAZGTR010000000
VERSION     DAZGTR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.043"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(259..663)
                     /locus_tag="ACHLCY_002201"
                     /pseudo
     CDS             complement(259..663)
                     /locus_tag="ACHLCY_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(746..833)
                     /locus_tag="ACHLCY_002202"
     tRNA            complement(746..833)
                     /locus_tag="ACHLCY_002202"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(797..799),aa:Ser,seq:tga)
     gene            1260..2378
                     /gene="hyaA"
                     /locus_tag="ACHLCY_002203"
     CDS             1260..2378
                     /gene="hyaA"
                     /locus_tag="ACHLCY_002203"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP0369457.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2375..4168
                     /gene="hyaB"
                     /locus_tag="ACHLCY_002204"
     CDS             2375..4168
                     /gene="hyaB"
                     /locus_tag="ACHLCY_002204"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP0369458.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4187..4894
                     /gene="hyaC"
                     /locus_tag="ACHLCY_002205"
     CDS             4187..4894
                     /gene="hyaC"
                     /locus_tag="ACHLCY_002205"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP0369459.1"
                     /translation="MQQKSDNVVSHYVFEALVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            4891..5478
                     /gene="hyaD"
                     /locus_tag="ACHLCY_002206"
     CDS             4891..5478
                     /gene="hyaD"
                     /locus_tag="ACHLCY_002206"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP0369460.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5475..5873
                     /gene="hyaE"
                     /locus_tag="ACHLCY_002207"
     CDS             5475..5873
                     /gene="hyaE"
                     /locus_tag="ACHLCY_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP0369461.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            5870..6727
                     /gene="hyaF"
                     /locus_tag="ACHLCY_002208"
     CDS             5870..6727
                     /gene="hyaF"
                     /locus_tag="ACHLCY_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP0369462.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            6861..8405
                     /gene="appC"
                     /locus_tag="ACHLCY_002209"
     CDS             6861..8405
                     /gene="appC"
                     /locus_tag="ACHLCY_002209"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP0369463.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8417..9553
                     /gene="appB"
                     /locus_tag="ACHLCY_002210"
     CDS             8417..9553
                     /gene="appB"
                     /locus_tag="ACHLCY_002210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0369464.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            9566..9658
                     /gene="cbdX"
                     /locus_tag="ACHLCY_002211"
     CDS             9566..9658
                     /gene="cbdX"
                     /locus_tag="ACHLCY_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP0369465.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            9738..11036
                     /gene="appA"
                     /locus_tag="ACHLCY_002212"
     CDS             9738..11036
                     /gene="appA"
                     /locus_tag="ACHLCY_002212"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP0369466.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11151..13331)
                     /gene="etk"
                     /locus_tag="ACHLCY_002213"
     CDS             complement(11151..13331)
                     /gene="etk"
                     /locus_tag="ACHLCY_002213"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP0369467.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13351..13797)
                     /gene="etp"
                     /locus_tag="ACHLCY_002214"
                     /gene_synonym="yccY"
     CDS             complement(13351..13797)
                     /gene="etp"
                     /locus_tag="ACHLCY_002214"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP0369468.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(13785..14924)
                     /gene="gfcE"
                     /locus_tag="ACHLCY_002215"
     CDS             complement(13785..14924)
                     /gene="gfcE"
                     /locus_tag="ACHLCY_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP0369469.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(14970..17066)
                     /gene="gfcD"
                     /locus_tag="ACHLCY_002216"
     CDS             complement(14970..17066)
                     /gene="gfcD"
                     /locus_tag="ACHLCY_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0369470.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17066..17812)
                     /gene="gfcC"
                     /locus_tag="ACHLCY_002217"
     CDS             complement(17066..17812)
                     /gene="gfcC"
                     /locus_tag="ACHLCY_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0369471.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(17809..18453)
                     /gene="gfcB"
                     /locus_tag="ACHLCY_002218"
     CDS             complement(17809..18453)
                     /gene="gfcB"
                     /locus_tag="ACHLCY_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP0369472.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(18560..18865)
                     /gene="gfcA"
                     /locus_tag="ACHLCY_002219"
     CDS             complement(18560..18865)
                     /gene="gfcA"
                     /locus_tag="ACHLCY_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFP0369473.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19307..19519)
                     /gene="cspH"
                     /locus_tag="ACHLCY_002220"
     CDS             complement(19307..19519)
                     /gene="cspH"
                     /locus_tag="ACHLCY_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP0369474.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            19805..20017
                     /gene="cspG"
                     /locus_tag="ACHLCY_002221"
     CDS             19805..20017
                     /gene="cspG"
                     /locus_tag="ACHLCY_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP0369475.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20028..20216
                     /gene="ymcF"
                     /locus_tag="ACHLCY_002222"
     CDS             20028..20216
                     /gene="ymcF"
                     /locus_tag="ACHLCY_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0369476.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            20191..20421
                     /gene="ymcE"
                     /locus_tag="ACHLCY_002223"
     CDS             20191..20421
                     /gene="ymcE"
                     /locus_tag="ACHLCY_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP0369477.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            20411..20584
                     /gene="gnsA"
                     /locus_tag="ACHLCY_002224"
     CDS             20411..20584
                     /gene="gnsA"
                     /locus_tag="ACHLCY_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP0369478.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(20632..21705)
                     /gene="yccM"
                     /locus_tag="ACHLCY_002225"
     CDS             complement(20632..21705)
                     /gene="yccM"
                     /locus_tag="ACHLCY_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0369479.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(21777..24521)
                     /gene="torS"
                     /locus_tag="ACHLCY_002226"
     CDS             complement(21777..24521)
                     /gene="torS"
                     /locus_tag="ACHLCY_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP0369480.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEKQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISTHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGIVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQSLETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            24604..25632
                     /gene="torT"
                     /locus_tag="ACHLCY_002227"
     CDS             24604..25632
                     /gene="torT"
                     /locus_tag="ACHLCY_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP0369481.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(25605..26297)
                     /gene="torR"
                     /locus_tag="ACHLCY_002228"
     CDS             complement(25605..26297)
                     /gene="torR"
                     /locus_tag="ACHLCY_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP0369482.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26427..27599
                     /gene="torC"
                     /locus_tag="ACHLCY_002229"
     CDS             26427..27599
                     /gene="torC"
                     /locus_tag="ACHLCY_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP0369483.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            27599..30145
                     /gene="torA"
                     /locus_tag="ACHLCY_002230"
     CDS             27599..30145
                     /gene="torA"
                     /locus_tag="ACHLCY_002230"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP0369484.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            30142..30741
                     /gene="torD"
                     /locus_tag="ACHLCY_002231"
     CDS             30142..30741
                     /gene="torD"
                     /locus_tag="ACHLCY_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP0369485.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(30834..31139)
                     /gene="cbpM"
                     /locus_tag="ACHLCY_002232"
     CDS             complement(30834..31139)
                     /gene="cbpM"
                     /locus_tag="ACHLCY_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP0369486.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(31139..32059)
                     /gene="cbpA"
                     /locus_tag="ACHLCY_002233"
     CDS             complement(31139..32059)
                     /gene="cbpA"
                     /locus_tag="ACHLCY_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP0369487.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         7648 a         8243 c         8493 g         7916 t
ORIGIN      
        1 aatatttaac gagaaataga agctttgtat cagacgggct tccctgaata gagaagttaa
       61 ataatcagga tgatgctaat gaatatcagg aacaaggtga gtgtcttcat gttcactgaa
      121 acagggtgtc acaaaaaata tggctataag atgatcaatg tgatttgaaa tatttatagg
      181 tttacagggg gctgggcatg catatcttac tgcaatgcag aatatgtgct gcttggcgca
      241 gctctttatt gagaatgttc aatcttttgt ttcagtttcg tgaaggatac ggacggtttc
      301 ttgtgtaata ttatacatcg cctgtttatc tttactgcat actcctgcaa gtgttaatgc
      361 tactccagca taaatttctg gataactatg tgtaaatata tccagtgagt ttacatgttt
      421 ctctttcatt cctgcatcac aacgaacctt atttatacct gtcgccattg attggggagg
      481 gctagagcct tctgcacgaa aatagtgatg tgacagatct gagatagctt tgtatccttc
      541 tgcctggttc aggcctggag cagtccatgt tttacaaaaa tactcggcag cgtaacgtgt
      601 tgcaagagcg gcatcagacg aggaacctga ggttccgctc atgaccgata atctcatccg
      661 aataaaaata taccaattgg tataccaagt ttataccaag agccaaaaaa caaaggggct
      721 accatttggt aaccccttga ttatttggcg gaagcgcaga gattcgaact ctggaaccct
      781 ttcgggtcgc cggttttcaa gaccggtgcc ttcaaccgct cggccacact tccggaatga
      841 cgcgcactat aaacatcccg atgcggcgtg taaaccccta atttgtttgt ttgcctgaaa
      901 aacagccaaa agtgcattga tagcgtgaaa taacagcaga ttgatcattt catcaccatg
      961 aattccttct cttttactcg tttagcaacc ggctaaacat ccccaccgcc cggccaaaag
     1021 aaaaataggt ccatttttat cgctaaaaga taaatccacg cagtttgtat tgttttgtgc
     1081 aaaagtttca ctacgcttta ttaacaatac tttctggcga cgtgtgccag tgcagaagga
     1141 tgagctttcg ttttcagcat ctcacgtgaa gcgatggttt gccttgctac agggacgtcg
     1201 cttgccgacc ataagcgccc ggtgtcctgc cggtgtcgca aggagaagag acgtgcgata
     1261 tgaataacga ggaaacattt taccaggcca tgcggcgtca gggcgttacc cggcgcagct
     1321 ttctcaaata ttgtagtctg gctgccacgt cgctgggatt aggcgcggga atggcaccaa
     1381 agattgcctg ggcgctggag aacaaacccc gcattccggt ggtatggatc cacggtctgg
     1441 aatgcacctg ctgtaccgaa tcttttatcc gctccgctca cccactggcg aaggacgtca
     1501 tcctttccct gatttccctc gattatgacg atactttgat ggctgccgcc ggaacccagg
     1561 cggaagaagt cttcgaagac atcatcacgc aatacaatgg caaatatatc ctcgcagtag
     1621 aaggtaatcc gccgctgggc gagcagggga tgttctgtat cagcagcggt cgaccgttta
     1681 ttgagaaact caaacgtgcc gctgccggag ccagcgcgat tatcgcctgg ggaacctgcg
     1741 cgtcctgggg ctgcgtgcag gccgcgcggc ccaatccgac gcaggcaacg cctatcgaca
     1801 aagtcatcac cgacaaaccc attatcaaag tacctggctg cccgccgatc ccggatgtga
     1861 tgagcgccat cattacttac atggtgacct ttgatcgctt gccagatgtc gacagaatgg
     1921 gccgtccgct gatgttctat ggtcagcgaa tccacgataa atgctatcgc cgcgcccact
     1981 tcgacgccgg agagttcgtc cagagttggg atgatgacgc tgcccgcaaa ggttactgcc
     2041 tgtacaaaat gggctgcaaa gggcctacca cctataacgc ctgttcttcc acacgctgga
     2101 atgatggcgt ttctttccca atccagtctg gtcacggctg cctgggctgt gcggaaaatg
     2161 gtttctggga tcgcggttcg ttctacagcc gcgtggtcga tattccgcaa atgggtactc
     2221 attccaccgc cgataccgtc ggtttaaccg cgcttggcgt ggtggcagcg gctgttggtg
     2281 tgcacgcagt cgccagcgcc gttgaccagc gcagacgtca taaccagcaa cctacagaaa
     2341 ccgaacatca gccaggcaat gaggataaac aggcatgagc actcagtacg aaactcaggg
     2401 atacaccatc aataatgccg gacgccgcct ggtggtcgac ccgattacgc gcatcgaagg
     2461 ccacatgcgc tgcgaagtga atattaacga tcagaatgtg atcaccaatg ccgtctcctg
     2521 cggcaccatg tttcgcgggc tggagatcat cctacaaggg cgcgacccgc gcgatgcgtg
     2581 ggcgttcgtt gaacgtatct gcggcgtctg tactggcgta cacgccctgg cttcggttta
     2641 cgccatcgaa gatgctatcg gtattaaagt gccggacaac gcaaatatca tccgcaacat
     2701 tatgctggca acgctctggt gccacgatca tctggtgcac ttctatcagc ttgccgggat
     2761 ggactggatc gatgtgttag atgcgctgaa agccgacccg cggaaaacct ccgaactggc
     2821 gcaaagtctc tcctcttggc cgaaatcatc ccctggctat ttcttcgacg tacaaaaccg
     2881 cctgaaaaaa tttgttgaag gcgggcagtt ggggatcttc cgcaatggct actgggggca
     2941 cccgcagtac aaactgccgc cagaagctaa cctgatgggc tttgcccact atctcgaagc
     3001 tctcgatttc cagcgtgaaa ttgtcaaaat ccacgcggtc tttggcggta aaaacccgca
     3061 tccaaactgg attgtcggcg ggatgccttg cgccatcaac attgacgaaa gcggcgcggt
     3121 cggggcagtc aatatggaac gcctgaacct ggtgcagtca attatcaccc gcacggcgga
     3181 cttcattaac aacgtgatga tccccgacgc cttagccatc ggtcagttca acaaaccgtg
     3241 gagcgaaatc ggcactggtc tttctgataa atgcgttctc agctacggcg cattcccgga
     3301 tattgccaac gactttggcg agaaaagtct gctgatgcct ggcggcgcgg tgattaacgg
     3361 cgacttcaac aatgtgctgc cagtggattt ggttgatccg cagcaggtgc aggagtttgt
     3421 cgaccacgcc tggtatcgat atcccaacga tcaggtcggg cgtcatccgt tcgatggcat
     3481 caccgacccg tggtacaacc ccggcgatgt caaaggcagc gataccaaca ttcagcagct
     3541 gaatgaacag gaacgctact cgtggatcaa agcgccacgc tggcgcggta acgcgatgga
     3601 agtggggccg ctggcgcgca cgttaatcgc ttatcacaaa ggcgatgctg cgaccgttga
     3661 gtcggtcgat cgcatgatgt cggcgttgaa cctgccgctt tccggtatcc agtcaacgtt
     3721 aggccgcatt ttgtgccgcg cgcacgaagc gcagtgggcc gcaggtaagt tgcagtattt
     3781 cttcgacaag ctgatgacca acctgaaaaa cggcaatctc gccactgctt ccacggaaaa
     3841 atgggaacct gcaacctggc cgacagagtg ccgtggtgtc ggttttaccg aagcgccgcg
     3901 cggggcgtta ggccactggg ccgccattcg cgatggcaag attgatctct accagtgcgt
     3961 ggtgccgacc acctggaacg ccagcccgcg cgatcccaaa gggcagattg gcgcttatga
     4021 agcggcgctg atgaacacca aaatggcgat ccccgagcaa ccgctggaga tcctgcgtac
     4081 tctgcacagc tttgacccgt gcctcgcctg ttcaacacac gtgctgggcg acgacggtag
     4141 cgagctgatc tctgtgcagg tgcgttaaca gcgaaggaga atcatcatgc aacagaaaag
     4201 cgacaacgtt gtcagccact atgtctttga agcgctagtc cgcatctggc actggttgac
     4261 ggtgttatgc atggcggtgt tgatggtcac cggatacttt atcggcaagc cgctaccttc
     4321 cgtcagcggc gaggcgacgt atctgttcta tatgggctac atcaggttaa ttcacttcag
     4381 cgccgggatg gtttttaccg tggttttgct gatgcggatc tactgggctt ttgttggcaa
     4441 tcgatactcc cgcgagctgt ttatcgtgcc ggtatggcgt aaaagctggt ggcagggcgt
     4501 gtggtatgaa atccgctggt atctgtttct ggcaaaacgt ccgagtgccg atataggcca
     4561 taatcccatc gcccaggcgg cgatgttcgg ctatttcctg atgtcggtct ttatgatcat
     4621 cactggtttt gcgctgtata gcgaacacag ccagtacgct atttttgcgc cgttccgtta
     4681 tgtggtggaa tttttctact ggatgggtgg caactcaatg gacattcaca gctggcatcg
     4741 gctggggatg tggctgattg gcgcgtttgt gatcggtcat gtctacatgg cgctgcgtga
     4801 agacatcatg tccgacgaca cggtgatctc caccatggtc aacggctacc gtagccacaa
     4861 atttggcaaa ataagtaaca aggagcgttc atgagcgagc aacgcgtggt ggtcatgggg
     4921 ctgggcaacc tgctgtgggc cgatgaaggc ttcggcgtgc gggtggcgga acggctgtat
     4981 gcccattacc actggcccga gtatgtggag attgtcgatg gcggtactca gggactgaac
     5041 ttgctggggt atgtcgaaag cgccagccat ctgttgattc tcgatgccat tgactacggg
     5101 ctggaacctg gaacgctgcg aacctatgcc ggagaacgca ttccggctta tctcagcgcg
     5161 aagaaaatga gcctgcatca gaacagtttc tccgaagtgt tggcgctggc ggatatccgc
     5221 ggacatctgc cagcacatat tgccctcgtc ggtctgcaac ccgcaatgct cgacgactac
     5281 ggcggtagcc tgagcgaact ggcacgggag caactgcccg ctgcggaaca ggcggcgctg
     5341 gcgcagctgg ctgcgtgggg aattgtgccg caaccggcta atgaatcgcg ctgtctcaat
     5401 tatgactgtc tgtcgatgga aaattacgaa ggcgttcgct tgcgccagta ccggatgaca
     5461 caggaggagc agggatgagc aacgacacgc catttgatgc gttgtggcaa cggatgctgg
     5521 cgcgcggctg gacgccagtc agtgaatccc gtcttgacga ctggcttacg caagcgccag
     5581 acggcgtgct gttattaagc agtgacccga aacgcacgcc agaggtcagc gataatccgg
     5641 taatgattgg cgaattactg cgcgagtttc ccgactatac atggcaggtg gcgattgctg
     5701 accttgagca gagcgaagcc atcggcgatc gctttggcgt ctttcgcttt cctgccactt
     5761 tagtgtttac cggcggaaac tatcgcggcg tgctgaatgg tattcacccg tgggcggaac
     5821 tgataaacct gatgcgcggg cttgtcgaac cgcagcagga gcgtgcctca tgagcgaaac
     5881 atttttccat ctgctggggc caggaacgca accgaacgat gacagtttca gcatgaatcc
     5941 actgccgatc acctgtcagg tgaatgatga accgagtatg gcggccctgg agcaatgtgc
     6001 tcacagcccg caggtgattg cgctgttaaa cgagttacaa catcaactaa gcgaacgcca
     6061 accgccgttg ggcgaggtgc tggcagtcga tctgttaaat ctcaacgccg acgatcgtca
     6121 ctttatcaat acgcttctcg gggaagggga agtgtcagtg cgcattcagc aggctgacga
     6181 cagtgaaagt gaaatacagg aggcgatctt ctgcggatta tggcgggtgc gcagacgtcg
     6241 cggcgaaaag ttgctggagg acaaactgga ggctggctgc gcgccgctgg cgttgtggca
     6301 ggcggcaacg caaaatctct tgccgacaga ttcgctgtta ccgccgccca ttgatggcct
     6361 gatgaatggc ctaccgttgg cgcatgagtt actggcacat gtacgtaacc ccgacgcgca
     6421 gccgcacagc attaatctga cgcaattacc catcagcgag gctgatcggc tttttctctc
     6481 acgtctctgt gggccgggaa atattcagat tcgtaccatt ggctatggcg agagctatat
     6541 caacgccacg gggttacgcc atgtctggca tttacgctgt acggacacct taaaaggccc
     6601 gttactggaa agttatgaaa tctgcccaat accggaagtg gtgctggcag cgccagaaga
     6661 tttggtcgac tctgcgcagc ggcttagcga ggtatgtcag tggctggcgg aagctgcacc
     6721 gacgtaaaaa gacggtaagt atcgctttca gtcttatgaa tatcgcaatc ggcgaatacc
     6781 tctggtcgta gagtttcagg ataaagaggg agatctacca ttatcgggtt atttttctct
     6841 cttcgcctac aggagtgcgc atgtgggatg tcattgattt atcgcgctgg cagtttgctc
     6901 tgaccgcgct gtatcacttt ttatttgtac cccttaccct ggggctgatt tttttgctgg
     6961 ctatcatgga aaccatttac gtggtcaccg gcaaaacaat ctaccgcgat atgacgcgct
     7021 tctggggtaa gctcttcggt atcaattttg ctcttggcgt ggctaccggc ctgaccatgg
     7081 agtttcagtt tggtactaac tggtcattct attccaacta tgtgggcgat atttttggcg
     7141 caccactggc gatggaagca ttaatggcct tcttcctcga atccaccttt gtcgggctgt
     7201 tcttcttcgg ctggcaacgg ctgaataaat accagcacct gctggtgacg tggctggtgg
     7261 cgttcggttc aaatctctct gcgttgtgga tattgaatgc caacggttgg atgcaatacc
     7321 cgaccggtgc gcattttgat atcgacaccc tgcgtatgga gatgaccagc ttcagcgagc
     7381 tggtctttaa cccggtcagc caggtgaaat ttgtgcacac cgtaatggcg ggctacgtga
     7441 ccggggccat gtttattatg gcgatcagcg cctggtattt actgcgcgga cgggagcgcg
     7501 atgtcgcatt acgttcgttt gccatcggtt ccgtcttcgg tactctggcg attatcggta
     7561 ccctgcaact cggagacagt tctgcgtatg aagtcgcgca agtacaaccg gtaaaactgg
     7621 cggcgatgga aggggagtgg caaacggaac ctgcacctgc accgttccat gtggttgcct
     7681 ggccggaaca ggatcaagag cgtaacgcct ttgccatcaa aattcccgcg ctgctaggga
     7741 tcctcgccac tcactcatta gataaacccg tgccgggtct gaagaatttg atggctgaaa
     7801 cctacccacg cttgcaacgc ggacgtatgg cctggctgtt aatgcaggaa atatcgcaag
     7861 gcaatcgtga gccgcatgtg ttgcaggcat tccgggaact ggaaggtgac ctgggctacg
     7921 gcatgttgct ctcccgctat gcgccggata tgaatcatgt cacagccgca cagtaccagg
     7981 cggcgatgcg tggcgcgata cctcaggttg cgccggtatt ctggagtttc cgcatcatgg
     8041 tgggctgtgg ttccctgctg ctactggtga tgctgattgc gcttgtccag acgctgcgtg
     8101 gcaaaatcga ccagcatcgc tgggtgctga aaatggcgct ctggagtttg ccgttgccgt
     8161 ggattgcgat tgaagccggg tggtttatga ccgagtttgg tcgtcagccg tgggcgatac
     8221 aggacatctt accgacatac tccgcgcact ccgctttaac cacaggacaa ctggctttct
     8281 cactgatcat gatcgtaggg ctttacaccc tgttcttaat cgccgaagtc tacctgatgc
     8341 agaaatatgc ccgtctgggg ccgagcgcga tgcagagtga acaaccgacg cagcaacagg
     8401 ggtaaaggag aaaatcatgt ttgattatga aacattgcgc ttcatctggt ggctgctgat
     8461 tggcgtgatc ctggtggtct ttatgatctc cgacggattt gacatgggga tcggctgtct
     8521 gctgccgctg gtggcgcgta atgatgatga acgccggata gtgataaaca gcgttggtgc
     8581 acactgggaa ggcaaccagg tctggttgat cctcgctggt ggggcattat ttgccgcctg
     8641 gcccagagtg tatgcagcgg cgttttccgg cttttatgtg gcgatgatcc tggtgctgtg
     8701 ctcactgttc ttccgcccgc tggcctttga ttatcgcgga aaaatcgccg atgcacgctg
     8761 gcgtaaaatg tgggacgccg gtctggtcat cggcagtctg gtgccgccgg tagtcttcgg
     8821 tatcgccttc ggcaacttgt tgctcggcgt gccgtttgcc ttcacaccgc aattacgcgt
     8881 ggagtatctc ggcagcttct ggcaactgct gacgccattc cctttattgt gcggattgct
     8941 cagccttggg atggtgattt tgcaaggtgg cgtctggtta caactgaaaa ctgttggtgt
     9001 gattcatctg cgttcacagc tggccaccaa acgcgctgca ctgttggtga tgctgtgctt
     9061 tttgctggcg ggttactggc tgtgggtcgg tattgatggc tttgtactgc tcgcccagga
     9121 tgctaacggt ccttccaatc cgttaatgaa actggtggca gtgctacctg gtgcctggat
     9181 gaataatttc gtcgaatcgc ccgttttgtg gatcttcccg ctgctggggt tcttctgccc
     9241 attgctgacg gtgatggcga tttatcgtgg tcgcccgggt tggggatttt taatggcgtc
     9301 attgatgcaa tttggcgtga ttttcacggc aggcatcacg ctgttcccct ttgtcatgcc
     9361 gtcaagcgtg agtccaatct ccagcctgac gttgtgggac agcacttcca gtcagctgac
     9421 gctgagcatt atgttggtaa tcgtgctgat atttttgccc attgtgttgc tctacactct
     9481 ctggagctac tacaaaatgt ggggacgcat gacaacagaa actctccgcc gtaacgaaaa
     9541 cgagttgtac taaggagcag gaacaatgtg gtatttactt tggttcgtcg gcattttgtt
     9601 gatgtgttcg ctctccaccc ttgtgttggt atggctggac ccgcgtctga aaagttaacg
     9661 aacgtaggcc tgatgcggcg cattagcatc gcatcaggca atcaataatg tcagatatga
     9721 aaagcggaaa catatcgatg aaagcgatct taatcccatt tttatctctt ctgattccgt
     9781 taaccccgca atctgcattc gctcagagtg agccggagct gaagctggaa agtgtggtga
     9841 ttgtcagtcg tcatggtgtg cgtgctccaa ccaaggccac gcaactgatg caggatgtca
     9901 ccccagacgc atggccaacc tggccggtaa aactgggttg gctgacaccg cgcggtggtg
     9961 agctaatcgc ctatctcgga cattaccaac gccagcgtct ggtagccgac ggattgctgg
    10021 cgaaaaaggg ctgcccgcag tctggtcagg tcgcgattat tgctgatgtc gacgagcgta
    10081 cccgtaaaac aggcgaagcc ttcgccgccg ggctggcacc tgactgtgca ataaccgtac
    10141 atacccaggc agatacgtcc agtcccgatc cgttatttaa tcctctaaaa actggcgttt
    10201 gccaactgga taatgcgaac gtgactgacg cgatcctcag cagggcagga gggtcaattg
    10261 ctgactttac cgggcatcgg caaacggcgt ttcgcgaact ggaacgggtg cttaattttc
    10321 cgcaatcaaa cttgtgcctt aaacgtgaga aacaggacga aagctgttca ttaacgcagg
    10381 cattaccatc ggaactcaag gtgagcgccg acaatgtctc attaaccggt gcggtaagcc
    10441 tcgcatcaat gctgacggag atatttctcc tgcaacaagc acagggaatg ccggagccgg
    10501 ggtggggaag gatcaccgat tcacaccagt ggaacacctt gctaagtttg cataacgcgc
    10561 aattttattt gctacaacgc acgccagagg ttgcccgcag ccgcgccacc ccgttattag
    10621 atttgatcaa gacagcgttg acgccccatc caccgcaaaa acaggcgtat ggtgtgacat
    10681 tacccacttc agtgctgttt atcgccggac acgatactaa tctggcaaat ctcggcggcg
    10741 cactggagct caactggacg cttcccggtc agccggataa cacgccgcca ggtggtgaac
    10801 tggtgtttga acgctggcgt cggctaagcg ataacagcca gtggattcag gtttcgctgg
    10861 tcttccagac tttacagcag atgcgtgata aaacgccgct gtcattaaat acgccgcccg
    10921 gagaggtgaa actgaccctg gcaggatgtg aagagcgaaa tgcgcagggc atgtgttcgt
    10981 tggcaggttt tacgcaaatc gtgaatgaag cacgcatacc ggcgtgcagt ttgtaatgca
    11041 taaaaaagag cattcagtta cctgaatgct ctgaggctga tgacaaacga agaactgtct
    11101 aatgcgtaga ccggaaaagg cgttcacgcc gcatccggcc actttcagtt ttactctttc
    11161 tcggagtaac tataaccgta atagttatag ccgtaactgt aagcggtgct ggcgcgttta
    11221 atcacaccat tgaggatagc gcctttaata ttgacgcctg cctgttccag acgctgcatt
    11281 gacaaactca cctctttggc ggtgttcaag ccaaaacgcg caaccagcag gctggtgcca
    11341 acagaacgcc ccacgaccgc ggcatcactc accgccagca tcggcggcgt atcgacaatc
    11401 accagatcgt aatggtcgtt cgcccattcc agtaattgac gcatccgatc gcgcatcagc
    11461 agttcagacg ggttaggtgg cacctgaccg cgagtaatca catcaaagcc tcctttgcca
    11521 aaatgctgga tcactttgtt gagctcatct ttacctgcca gatattccga caagccatgt
    11581 tcattactca cggtaaacag gttatgcgaa taaccacggc gtaagtcggc atcaataaat
    11641 aacacttttt gatcggactg ggcgatcacc gctgccagag ttgaactgac aaacgtttta
    11701 ccactgtctg gcgtcgcacc ggtgatcatc agaatgttat tctccgtctc catcatagcg
    11761 aaatgcagac tggttcgtag cgcacgtacg gcttccacag cagaatccgc cgggttatcc
    11821 accgccagga aggggatatt tttagtacga tggcgctgct gattagaaaa taaatttttc
    11881 ttacgcagac gggtgcgttt atccagccac tcggacattg ggatagtggc ataaacgctg
    11941 atgccgtgct cttccagttg ttccggggct tctacaccac gacgcaacat cgcacgcgcc
    12001 agcacggcac ccacagaaat aaacaggcca agaataaaac caagcaccac attcaacgct
    12061 tttttcggtt tcactggctg cggctgagtg actgccgggt cgataatccg cacgttacca
    12121 atggcactgg atttcgaaat actcaactcc tgctggcggt taagtaattg cagatatacc
    12181 gcacggcccg cttctacgtc acgacttaaa cgcaacactt cctgttgggt ggaaggcatt
    12241 gccgataccc gcttattcag gcgtttgcgt tcttgctcca gcgtctggcg tttttccagc
    12301 agcgcacgat aagttgggtg atctttctta tacagctggg agatctctgc ctcgcggaag
    12361 gtcagctcat tgagttgatt atcaacgttc acaatctgct caagaacggc tttggcttcc
    12421 aggttaaggt caaccgaatc gcgctgctgg cgataaacgt tgagtttttc ttccgcctgg
    12481 tccagctcgc tgcgcacttc aggtaactgg cgctgtaaga attcaaggct ttgtgaatcc
    12541 tgcgccgcct ggcgagcgat attctgttgc aaatagttgt tagcgatgct gttcagaata
    12601 cgagtaatca actggggatc atcaccagtc atggtaagtt ccagcatccc gctttcttta
    12661 ctgcgttcgc taacggtaaa ggtttcctgc aatgcgttaa tcgcttccag ttcggtacgc
    12721 tggctcagga caaactgtgt tcctggtttg gccttaatgt ccgcgatagt cagcgcaacg
    12781 ccatcttttt ccagacgctg gccgaccata ccattgacgg tgaactcttc accttccagt
    12841 gtatagtggc cgttttcccc aaccgtgagt gtcagttgct gatcctgacc attcagttgt
    12901 ggaatatgca tccagctgat cgccagctca cctggttttt ctttggttaa tctcgcccag
    12961 ccgcgaccca caatcggaaa atacttctgc tcaactatgt cgcgcagatt cagttcagca
    13021 atggttttac cgagaatcat gcgcgattgc agcagttgga tctccggtgc agactcgggc
    13081 gatgagttag ggatcatatc gctcaggccg ctgagaatgg cgttgccctg tttttgctca
    13141 acctggacca gagtatctgc ctgataaatt ggtgtgctta aaagcgagta agcgacagcg
    13201 atcagcgtga ataacgcggt cacgctgata ataaacttac ggtgatccca taactcgccg
    13261 accagacgaa gcagatcgat ctcattttcc tgagtgctgc ctggtggcgt attcatattt
    13321 ttagttgtca tcgtatttta taaactcaaa ttaccggctg aggcgcttcg cccattcctg
    13381 actggcgcgc tccaacatac cgtagacatg ttcaaatgcg tcctgacttt tacgataggg
    13441 atccgggatc tctttctgtt ccagccattg cccaaacagc attgtcttcc cgcggacctc
    13501 gggagcaatt gcggtaacct gagcaatatg ttccgactcc attgccagaa tcagatcgta
    13561 atttcgtgcc atctcagcag tgagcttacg tccggcatgt ccttcaagag atacgccatg
    13621 attagcggca acatctgccg ctgtcgcatc tgcagggtgt tttaccagac catgaacacc
    13681 tgccgatttg acttttacgc ccggcagacg tttacgcagc aagcgctcgc caatgggcga
    13741 acggcaaatg ttgccggtac aaaccaccag gattgagtta aattttagtt gggccatgtt
    13801 tgaatccacc gtgaagtctc tgtcaggtca tgcaccccgg aaatcgtcgg taccagcaga
    13861 gaaatcacac ggttccaacg agccagcggc gcggtagtga cataaacaat gtcgtaaggt
    13921 tcaagctgga attccgttcc gagtatcatt gccgatgcgt ccttcgcatt cagctgataa
    13981 atattggcga ttttgccatt ctgcttgttt tgcgttgcac gaataacaaa aatccccgtc
    14041 gcatcagcga catcctgatt cataccctca gcgttaccca gcgcttccgc cagggtcata
    14101 ccgctgcggt ccattttcag cgtgctctgt ttacccactt cacccatcac gaagactttc
    14161 agatcatcgt tgcgcggaat aaataaaata tcgcccgggt gcagcagttt gttctggcgc
    14221 aagtcgccac gctgcatcag cgcatagagg ttcactttgg tctttacgcc attctgggtg
    14281 agcaccacgt tgcgccagtc tgcatctgcg gtcagaccgc ccgcggcgtt aatcgcatcc
    14341 atgatggtca gaggaatatt ggtaatcggc tgctgacccg atttgctgac ttcgccggtg
    14401 acgtacgctt tttgcgagcg gaacgccgcg acgctgacat cgacttgcgg gctttcaatg
    14461 actgaatcca gacgcgctgt aatctcgttg cgtacctggg taagtgtttt cccggccact
    14521 ttcaggcgac caatataggg gtagaaaatc gcaccatcgg cgttaaccca gttgccggta
    14581 tcactggcgc tacggtattg ccctgcgggg gtggttagtt ccgggtgatc ccagaccgta
    14641 accatcaaca catccccaat gccgatacga tattcatagt ttgcgatttg ctgctccagc
    14701 tccgggttag cctgcgacat aatcggtttg gcgcgcaact gatcaatcaa tcctggcgtg
    14761 acgggataga tattgaccat tttgtccagg tcatactgat tgtcaggtag ttcaatgaca
    14821 tccttgttac tggtcgataa attctgacca ggaacaaggg tacatgctgt caaagacaga
    14881 acagccaatg tcagtaccga aaacttaaaa atgttctttt tcattggttt atatttatca
    14941 gtcaatgtaa tgttatgaat tacgtcaggt tattgtccaa ccggtacttc tcgctctgcc
    15001 gtcattggat agagctggta tttgcgtgac agcatttgtc caccatcacg cgtcaatggt
    15061 gtccacgaaa ccaccgcgcg gttgcggtta ggtccaatgg tcatcaagtc gaatggaatc
    15121 gagatataaa aacctttact aaagccgcct tcgccgtagt catctttaga tacgttactg
    15181 attgccgccc atacccctac cgccacgccg ctgtcaaagc gtttggcgac gtcgatcgtt
    15241 gcgcctttat cttttgccag atattgccca acgctaagtt tcatcagtac gccattgagc
    15301 gtcggcgggt tccagtaagc cgtcacgaaa cctgttggcg tggaataatc ggtgaaacgc
    15361 atcatgttgt cccagtcacg ttgcttcacg tagttaacgt ccacacccag cgcccagctg
    15421 gcatctagcg ggcgataaag cagctcggaa ccgacaccgg cgtacatcgt ttccagataa
    15481 ccgccataca cctggccata gaaaccattg cctaagtcgg caaagtagtt cgcctgcaag
    15541 ttgttgagat aaacgtcatt gcgaacgtaa tcacggatat gcgtgcgcac gcggggcagg
    15601 gtagagtccg cgggcaatag cgaagactta aacttgtcgt agttgttgta aatattggtg
    15661 aaaataccgc catcaagcag caggtggtcg gtaaaccagt agcgggcact ggacatcagc
    15721 cccagctgga acatatagaa atcttccggg ccgcccagcg actgtgaaag tgttgggttg
    15781 aaagagtagc taaagcgatc ttcacgaata cggtagcccc gaccaaaggc agaaagatct
    15841 tctgcttcaa cacgttgttg ttgaagttgc tctgattgac caggcgctgt accagccagc
    15901 tgtttgcgca ggctggctac gtcggtttcg gtagtcacca tcgccatatg ctcgcgcttt
    15961 tgcgtcacgc taatcttctc aacgccttgc ggcaggttat tcaccagaat ccggttggca
    16021 cgatcgaccg cttcgcgaga atcacggtat ttgtattgtt gaccagacat atacagcgtc
    16081 ttatcgcgca gctgaatttc cggtgcttca aaacctgcgt tgtacttcag cgcggtaagt
    16141 tggttcgcta ctgtggtgta ctgcaatcct tcagattcag gcgcaggttg atatgccggt
    16201 tttggcgtat cgcgcagggc agggcgcaga tcgttgaaat tggtccgtaa cgtgaagcca
    16261 aacatcaacg tgttaccgcg ttcataactc aggttgagat ctgcccagct ggcagcgcga
    16321 taaactgcgc cgacgttgaa atggcttgcc tgaggcagtt tgccagcgaa atcattctgg
    16381 taattgttgc catcgtattc gagtttcaga cgcaggggat tccacggcgt ttgatactca
    16441 atgccgccaa agatggaagc cgggccacga aagatatcgc taaagctgat atcgcccgca
    16501 tcgtgagact ctgcgcgatg acaatattta tcgctcaccc ggcaaaacgg gttggtaata
    16561 ttgcccgcat taccggcgta gccccatgcc atcccgaggg tgaaatcaaa tggccccgct
    16621 tgcttgctgg ccaccagata ctcaccgtca aacagacccg taccagcaat atcacgttta
    16681 ccaaacgcca cttgcggtag ccagtaacct tcttcccaca ggcgaagttt aaaatcgaat
    16741 gatttgtctt tgtatgactg atcgccgctg aaatcttccc actggctata tttgcgtgtg
    16801 cgcacatccg tataacgaat ggtgccttcc agccatggga acagcgccac ggaggtggag
    16861 tagaaccggt attgatcgtt atcccggtag ttgacgctga attcaccttc cggtgcgatg
    16921 cgcgcatttg gcatctgcaa caatcctgtg ccgccaaagt ctgattgcga cgaacccagc
    16981 ggatccgggt aggttaatac ttcagcatga caggcactgg agacggcaat ggccaggcag
    17041 cttaaaagat aagaattctt cttcatcagt caggaacacg ttgcgttaaa acagacacaa
    17101 tctggtcatt taaatcagca tatttttcgg gcagtacatg cgcagaaaag cccaaccaaa
    17161 gctggctgcc aggcggtggt tccacatgtc ttttattcca cagggcaaca ggggcgacga
    17221 cagtctcacc ctcaggcgta atcaccataa cgttattctt gtccgcgcct gcaaggcggg
    17281 ggtgatcttg cagataatcg gtaacgctac ggcctgcctg ccagggcaat tgcccggccc
    17341 cagaaaccgc acccagcaga gtaatagtca ccggacgctg tacggtatag agcgtatagt
    17401 ccccgaccaa cgggggattg ctgttctcat ccactcgtac aaaatcaggg tccagtttga
    17461 ccggtaagcg accagtgata ttgagattga gtaactgctg gcgcacggat ttaatcgttg
    17521 ccgccacatc gtcgtcagct tctgcttccc aggaagcaag ctgtgccatg acatgttggt
    17581 agtctttaag cgctttagct ttcgccgcag agtcggtcag caaggcacca ggccaccaga
    17641 ggcgatcgcg cagttgcggt tgtgtcacca actggacaac attttccatg gggccaacgg
    17701 aaagtgtctg ttgttcgcca ggcagataaa tagtcaccgt tccttgcgca aaggcatggg
    17761 gtgtcattac gtaaagtacg ctggcaatga aatacgactg taatttattc atggtgcggg
    17821 tttaagaaac gtcatttcta cgggaataac ccctgcgcct aacatttgcc gactttgacg
    17881 aacctggcca gtagccgaat cgacccagaa tgtgttttgc cactgttttt ctggccgcgt
    17941 ggaagtcacc tcttcctgcc agacattaca cagcacattg cgacccgcaa tattcagcgt
    18001 ctctaatcca gcaaaggaaa aagtagaact cagggtcgca gagcgaaaat ggttgtcttc
    18061 actccagaga atatcgcgcg tccagcggct gccatctttt attgccagcg cgttacgcag
    18121 agggtcctgc ccggaatttg tcacttccag cagattatta ttaagtttga cggtttttaa
    18181 aagacgtcca ttgtgggtaa ccagcatggc gttatcctgg gataaccatt tgctttgttc
    18241 ttgttctata tatcccagta ccacaaagat tcgttgccct tcattaaggc gtaaatacat
    18301 cgtggaataa ggcaacgcct ggatctgctg atccgctacg gtgatatcct gattatcgaa
    18361 aaggctggcg cgaaatgtat cgaccatact ttgctggctg tgcgtacatc ccgcaagaaa
    18421 tagtgcgaaa atcgataaaa taagagggcg cacctttaat ccttgaaagg ttaattaaaa
    18481 aaatgcaaat ctctaaagcc cggtacgaat atcgcaaccg ggtatcattt aactttaatt
    18541 tcgacgcgtt aaagcagtat tagcgtgtag tggacgtggt ggttgaggtg ttatgagaac
    18601 cgccatcgcc actgtcattt gctgcaacaa ctgccaacgt cgccacacca acagccgttg
    18661 ccacaccgac aactgtcgcc gtgccgacag cgccagcagc cacgccacca gtggtagtag
    18721 cagtcgtggt tgcgccggta gtggtcgttg tggtgccagt agtacccgtc gttgcctcgg
    18781 tcccgtttgt tgcttcaggg gcagcaaacg cagctggcgt cgtcaacatg aaggccataa
    18841 ggattgcaga aagcttgtgt ttcataactt ttcctttatt catcgcatgg acaatacgta
    18901 cgcttgagat caggattgtc tactttgagt ataacatcct tgtgaaatct ccctttcctc
    18961 ataccgcttg cactatatac tgcgggtagg aaaggcgcgc agagggaaat ataagattgt
    19021 taactatccc cctctggaaa tacgacttgt gagcataaga taaatcttag caaaatacaa
    19081 attaaagata aggaaattgt tacgaaagct attaattatt gttggtaata tagtttcaag
    19141 tggtactata actggctgtt aaatggcatt tataactatt aggtgcagag atattcgctt
    19201 aaatggatta gtttctttgt ggaaaagtaa ctgatgttat aatgtatgac gttgactatt
    19261 gcactattcc aataaaacca gtcagcttta aacaagcagc gtcatattaa gagagataaa
    19321 catttgccgc tgttggtcct cgcaggccat ttacgcggca aaattccaca cgtaatcctg
    19381 gtataagcac ttctgcgtcg cggggagtga atgcggaaat atggacctga acttctttac
    19441 gaccgtcgga ggggataatg aatcctttgc cgcttttgcg atcaaaggtt ttgacaattc
    19501 ctgtcatttt acgggacaaa caaattcctt actgaaaata ctgcgctgca ctatacgggg
    19561 ttaataaaat aaagccagcg atatttaaga ccgccggacg gctaaaataa aatttgctta
    19621 atctcaatta tcatgcgtta atagctgcgt cggtttgaaa gacagacagc atacaaagta
    19681 gtttactaaa gcagttctca ttatcaggca ttatcccctt cttttgagtc tctctcctga
    19741 acactaagta gtttctgtat taaagccctg tttgccgaaa ggcccaaaat gaaggaagta
    19801 aaatatgtct aataaaatga ctggtttagt aaaatggttt aacgcagata aaggttttgg
    19861 ctttatcact cctgatgatg gcagcaaaga cgttttcgtc catttcactg ccatccagag
    19921 caatgaattc cgcacgctga acgaaaatca gaaagttgaa ttttctattg agcaggggca
    19981 acgtggcccc gcggcagcga acgttgttac gctctaaggt tgccgttatt actcaacatc
    20041 tccatttccg ctgtccatgt tgtcatggtt cacagtaccg tacatcggca ttcgatgtga
    20101 cggagcgaaa ccctttgggc gctaagtgta ttttttgtaa atcgacgatg atcacctttg
    20161 ataacgtcgc gctgcaaata cgcacggacc atgcgccgct ggatttcaca aaataatatc
    20221 aggctccctc gtggagcctt ttttatatct gccttatttt tcttcaacgc tgtatgtata
    20281 gtaagcgata acctgttgat tattgaatct ttcggggaga tggcttataa catttcttac
    20341 ctgaccaggg taccgggaac caacacctta ctggcgtgtt gctgtctttc aagaccagaa
    20401 gaggttaaca gtgaatattg aagagttaaa aaaacaagcc gaaacggaaa tcgccgactt
    20461 tatcgcgcaa aaaatcgccg agctgaacaa gaatacaggg aaagaagtct ctgaaattcg
    20521 cttcaccgca cgagaaaaaa tgaccgggct tgaaagttat gatgtcaaaa tcaaaataat
    20581 gtgattttgt gaacatcacc cgtgcgaggt gatgttccgc ttgttgctaa tttagtgacc
    20641 aatcattggc gcttgtggaa ttaagcgtcg gtacaattca tccggtaccg ggctttgcca
    20701 tactcccgca tacattgcgt aaccaatcac cgcaaacata atccccagaa ccagtagcgt
    20761 cattaaccag ccagacaacg caaaggcttt tttatttgcc gcaggttttt gcagtgaaaa
    20821 ggtcaatgtt gaggctaccg gacatgactc tacgcaagtc atacagccgg tacattccac
    20881 tgttcgtacc tgaattaatt tatcgaccgg gatccgtgat gggcaatttt ttgcgcattt
    20941 gccacagtcg atacaacttt cggcattgcg acgaatctta aacggcgaca atagcgaaac
    21001 cacgcccatc agcgcgccat atgggcaaag ataacgacac caggcatggc gaataaacag
    21061 gctggcaatc agcaaaacgg tcacgctgat taatgtcgcg gtccccatat gacgaaagaa
    21121 atcgagcatt ttaacgtcca tcaccacgct gtagggcgac aacataaaat agtgaatcgc
    21181 ctgagcgggc atcaataatg cgatatagag aaaaaaactc aacagcaaat acttcacgcc
    21241 gcgcagagga atatccagcc agcggggaag gacacattgc cgaccaaaca gtttgttacc
    21301 gagatcgccg attaattcag aaagcgtacc aaccgggcat aaccatgagc aaaaggcctt
    21361 tttgagtaat agactgatga cgataaaagc gaccaataac agcatcgcgg cggcgtggac
    21421 ggacggtaac tgacctgttg taaggctata tttcagattc atcagaccgg caatcggtag
    21481 ccagccttcg atacctcccg gtctggcgac aaatgtcgtg ctacttgccg tttcgtaata
    21541 gcgcacccaa taccagaacg tgatggcaat ataaatattc attgccaaca gtaataattg
    21601 cgtcgcttta cgccaggtcg tggcattgcg ccagtcattc cacggtaatt tgccgcctgt
    21661 cgtgcctggc cgccgctgcc agcgggttct ttttttctct gccatgattt tgccagtccg
    21721 ttaagttgca taccaaatgc cactattcta gttgttctta actggctgat attgattcaa
    21781 atcgcgttca ggtctttctt attcaaccat gcttccagat cggcaacact gcgtgtaatt
    21841 tcttcgtgtg gaaggggggc agataatggc tgctgctcca gttgtgcgca tagctggctg
    21901 gcgatacgca ttcccaaact cgagcaactg ctttttagct gatgcgcggc acgctttatt
    21961 ttctcgctat cctgactggc gcgggcaatg tcgatttcat cgagaagcgg cagggcatgt
    22021 tgtgtaaata ataccagcca ttcgtggatc ttctccgtcc ccattaactg agcatcttca
    22081 ttgagttgcg atacatccag cgattgatcg ttattgactt gcagttggag atagtgcgcc
    22141 agtaactgac cgagcacttc acgcggcacc ggtttaggga taatcccgcg gaataatgaa
    22201 ctggtacgct ggcgcagcgt ttcgtcaatg acatgggcgc taaagccaat caaaaccagc
    22261 gacggatatt gctgtgccag ttgtcgggca agcgtaatgc cgtcgacatc cggcagatca
    22321 aaatccacca gtgcggcagc aaacggttcg ctattttgca gtgtctctaa agactgcgcg
    22381 gcattgccaa cagcaacaac ctgcgcacca ctggtgttca gcatctcaac ggtaattcgc
    22441 tgggttagcg ggttatcttc aattaacagc aaacgtaaac cgtcaagacg caccgcctga
    22501 ttgactgttt ttgggacggg tgccgtggca acacgtaacg gcaagcgtaa acaaaaacag
    22561 cttccaacct ccggcgtgct ggtggcgctc agttcgccgc ccatcgcctg ggccagacgg
    22621 ctactgatag tcagccccag cccggtgccg ccgcgtttgc cgcttacctg cacaaatggc
    22681 tggaagattt ctgccagttt cgcgggatca ataccgcagc cgctgtcttc cacttcgacc
    22741 agccattgct cgccatcagt gcgactacgc aggataatgt acccttcgtc agtaaaacgc
    22801 agggcgttgc tcaacaggtt ggttataacc tgacgaatac gtcgtggatc gcccattaac
    22861 gcgcacggca tatcatcggc aattgccgtt gccaggcgaa tcgggcgacc tttcacccgt
    22921 ccgctcatta attgcagggt actttccagc agcgggcgcg gttcaaaggg ctcatcgctg
    22981 accgaaacat tcttgccacc tgcttcgata gcggaataat cgagaatatc gttgaggatg
    23041 gtcagcaacg attcaccaga gtcagtaatt gcccgcaaat catcacgctg ggcgttaagt
    23101 gcggggttat ctgccagcag ttgagcagtg ccgagaatac cgtacagcgg tgtgcggatc
    23161 tcatggctca tcgccgccag aaacgccgat tttgcctggc tggctttttc tgcttccgcc
    23221 cgtgcctgtc ggtgttctat caccagttcc tgcaattcag ctgtacgcgc tttgacctgc
    23281 gccgccagct gttcgcggtg gcgattcagt gcatgaacat tgctgcgaaa cgcatccatc
    23341 agccgcccga tggtatccag ctcccgtacg ccagcggttt ccgggaaagg ggagtcaata
    23401 tcaccgtcca gcagccgttg cagcgcctgc gtttgttcgg caagtggacg cgtgactgag
    23461 cgataaacca cgcgccagag gatcagaatc agtgcgcaaa gagaaactat tcccagcaat
    23521 aacaggctgt attgcccgcg tgcactcgct ttttccagat gcgccagtcc gtgctgatta
    23581 cgcagctcaa tggtatcgac cagctgactg acttcgctac taaactgcgc gaactgggcg
    23641 atgttatttt gggcgagagt ttgcaggtga gtgctgattt cactgtcctg ctgatacagc
    23701 gccagtagat cgctatattg gctaacggta gttaacgttg ttgcgacctg cgcacgaact
    23761 cccggatctt caatgcgtat ttgccgacgt tgcagaattt tcaccgcatt attgagctgc
    23821 ttttccagcg ttggtgcatt tttctggatc tgctccagcc ccagattcat caccatttgc
    23881 tgcacccgca gagcgctaag gcgcagttca ttcatctggt taacatactc aagatcgata
    23941 tcaatcagcc gatcgagtgc actttcagca gcctgacgct gatcttgttc gatcaaatcg
    24001 taaatcccgg cctgggtcgc tccagcggaa gttgtcgcat tattcgcctg accttgcgcc
    24061 aggcgtgcga tctcatcggc ggcagcgact atctgctgac tgagttgccg ttgttgctgg
    24121 cgtagttgca gacgccgccc caccagttcc ccttgctgac gtaatgaacg ggagatctcc
    24181 tgctcctgtt tttcaatagc ggtggtatca aaaccttgtt cccgtaacgc ttgcagcaac
    24241 gcattaatct tcaggctttg tgcggtgagc attcgcccct gcgcctgcca catcttttcg
    24301 ttatcggcac tggtcaggtt ctgcgcggcg aaaagttccc aggcgctggc ttcgctcaac
    24361 tggcgcgcca tattcatggt aggaatcaat gcctgagtgt tgtctttttc cacctggctg
    24421 ataaagcgca ggttgtacca tcccaccagg gtactggtca gggttaacag cgccatcagg
    24481 gcaaagccca tccagagtct tcgggtcagg gttaaattca cggtcggtgc acttaaggtg
    24541 aaaaaggttg agtcgcaaag cggaatgcat ctagcataaa gccttattat tgatgaggct
    24601 atcatgcgcg tactgctatt tttacttctt tcccttttca tgttatcggc attttcggct
    24661 gataacctgt tgcgctggca tgatgcgcag cattacacgg tgcaagcctc tacgccgctt
    24721 aaagccaaac gcgcatggaa actgtgcgcg ctttatccca gcctgaaaga ttcatactgg
    24781 ttatcgttga actatggtat gcaggaggca gctcgccgct atggtgtgga tttaaaagtg
    24841 ctggaggcag gcggctacag ccagttggct acccagcaag cacaaatcga ccagtgtaaa
    24901 cagtggggcg cagaggccat tttgctcggt agtagcacta cgtcatttcc cgacctgcaa
    24961 aagcaggtag caaatctgcc ggtgatcgaa ctggtaaatg ctcttgatga tcccctggtg
    25021 aaaagccgcg ttggtgtacc ctggtttcag atgggctatc aacctgggcg atatttggtg
    25081 caatgggcgc acggtaaacc actgaacgtg ctgttgatgc ccggacccga taatgccggg
    25141 ggcagtaaag agatggtcga gggttttcgc gcagccattg ccggaagccc ggtgcgtatt
    25201 gttgatattg cgcttggtga taacgatatt gaaatccagc gtaacctgtt gcaggagatg
    25261 ctggaacgcc acccagaaat cgacgtcgtt gccggaacgg ccattgcggc agaggcggca
    25321 atgggtgaag ggcgtaacct gaaaacgccg cttaccgtgg tgtcgtttta tctttcacat
    25381 caggtgtatc gcgggctgaa gcggggaaga gtgattatgg ctgccagcga tcaaatggtc
    25441 tggcaggggg aactggcggt tgagcaggcc atcaggcaat tacaggggca atcggtgtct
    25501 gataatgtca gcccaccgat tttagttctg acgccgaaaa atgccgaccg tgaacatatt
    25561 cgccgctcgc tgtcaccagg gggatttcgt ccggtctatt tttatcagca cacatcagcg
    25621 gctaagaaat aaccttcacc atgttgcgtc accagtaaat ccgcgctgag tttatgacgt
    25681 aaacgacgaa ttaacacatc gacggtgcgc aggtcagggt tttccacccg acgcgcagaa
    25741 agcatacgta gcagacgttc acggctgaga atttcgcccg gattcgtcac aaatgccacc
    25801 aacatttcat actctgcgcg ggtcagttta atcggctcgc catcccgctc cagcgtatgg
    25861 cgcgacacat tcaggcaata accggcaaag cgatagcagt tgtcctgagt gtgcggttga
    25921 gcttgtcgcg cgaggtcgat tcgccagagc agatttttca cccgtactac cagttcgcgc
    25981 agttccagcg gtttggtgac gtaatcgtct gcgcccattt ccagcccaac aatacggtca
    26041 atccgatcgc tgcgtccggt aaccagaata atccccaccg ttgagcgttc tcgcagggcg
    26101 cgggttaaca tcaggccatt ttcatcgggt aagttgatat ccagcagaat taaatctacc
    26161 ggctgattct gcataatttc ccgcagcccg gcaccgctcg ctgtaacgga aacggtatac
    26221 ccctcctgag tgaagtaaga ttgtaatcgc gcctgggtaa ccggctcatc ttcaacaata
    26281 acaatgtgat gtggcatcag agggttttac tcattctgtt catatctgtt catattctgc
    26341 cgtaagccgt tcatcctgac cagtgccgct gttcatattt gctcattaag atcgcttcac
    26401 taaaccataa ttttacaggg gctattatgc ggaaactctg gaacgcgcta cgccgaccca
    26461 gtgctcgttg gtcggtactg gcgctggtcg caattgggat tgtgattggc attgcgctga
    26521 ttgtattgcc acacgttggg atcaaagtca ccagcacaac cgaattttgt gtcagttgcc
    26581 acagtatgca accggtgtat gaagaatata aacagtcggt gcatttccag aacgcctccg
    26641 gcgtgcgagc tgaatgccat gactgtcata tcccgccgga tattccaggc atggtgaagc
    26701 gcaaactgga agcgagcaat gatatctacc agacctttat tgctcattcc attgatacac
    26761 ctgaaaaatt cgaagccaaa cgcgcggaac ttgccgagcg tgaatgggcg cgaatgaaag
    26821 aaaacaactc ggcaacctgc cgctcctgcc ataactacga tgcgatggat catgcgaagc
    26881 agcatcctga agcggcacgt cagatgaagg tggcagcgaa agataatcaa tcctgcatcg
    26941 actgtcataa aggtattgcc caccagttac cggatatgag tagcggcttc cgtaagcagt
    27001 tcgatgagct gcgcgccagt gctaatgaca gtggtgacac gctgtactct attgatatta
    27061 agccaattta tgcggcgaaa ggcgataaag aagcctctgg ttctctgctg cctgcttcgg
    27121 aagtgaaagt ccttaaacgt gacggcgact ggctgcaaat tgaaattacc ggctggacgg
    27181 aaagcgccgg acgtcagcgt gtactcaccc aattcccagg taaacgcatc tttgttgcct
    27241 cgattcgtgg tgatgtgcag cagcaggtaa aaacgctgga gaaaaccacc gttgccgaca
    27301 ccaataccga gtggagcaag ttgcaggcca ctgcgtggat gaagaaaggc gacatggtga
    27361 acgatatcaa accgatctgg gcttatgcgg attcgttgta caacggcacc tgtaaccagt
    27421 gccacggcgc accggaaatc gcccactttg acgctaacgg ttggatcggc acgctcaacg
    27481 gcatgattgg ctttaccagt ctcgataaac gtgaagaacg caccttgttg aaatatctgc
    27541 aaatgaatgc gtctgacacc gcaggtaagg ctcacggcga taagaaggaa gaaaaataat
    27601 gaacaataac gatctctttc aggcatcacg tcggcgtttt ctggcacaac tcggcggctt
    27661 aaccgtcgcc gggatgctgg ggccgtcatt gttaacgccg cgccgtgcga ctgcggcgca
    27721 agcggcgact gacgctgtca tctcgaaaga gggcattctt accgggtcgc actggggggc
    27781 tatccgcgcg acggtgaagg atggtcgctt tgtggcggca aaaccgttcg aactggataa
    27841 atatccgtcg aaaatgattg ccggattgcc ggatcacgta cacaacgcgg cgcgtattcg
    27901 ttatccgatg gtacgcgtgg actggctgcg taagcgccat ctcagcgata cctcccagcg
    27961 cggtgataac cgttttgtgc gcgtgagctg ggatgaagcc ctcgacatgt tctatgaaga
    28021 actggaacgc gtgcagaaaa ctcacgggcc gagtgccttg ctgaccgcca gtggttggca
    28081 atcgacgggg atgttccata acgcttcggg gatgctggcg aaagctattg ccttgcatgg
    28141 taatagcgtt ggtacgggcg gagattactc taccggtgct gcgcaggtga tcctgccgcg
    28201 cgtggttggc tcaatggaag tgtatgaaca gcaaacctcc tggccgctgg tattgcagaa
    28261 cagcaaaacc attgtgctgt ggggctctga tttgctgaaa aaccagcaag cgaactggtg
    28321 gtgcccggat cacgatgttt atgaatatta cgcgcagcta aaagcgaaag tcgccgccgg
    28381 tgaaattgag gtcatcagca tcgatccggt tgtcacatcc acccatgagt atctggggcg
    28441 cgagcatgtg aagcacattg cggttaaccc gcaaactgat gtgccgctgc aactggcgct
    28501 ggcgtatacg ctgtacagtg aaaacctgta cgacaaaaac ttcctcgcta actactgtgt
    28561 gggttttgag cagttcctgc cgtacctgct gggtgagaaa gacggtcagc cgaaagatgc
    28621 cgcatgggct gaaaaactga ccggcattga tgccgaaacc attcgtgggc tggcgcggca
    28681 gatggcggcg aacagaacgc aaattattgc tggctggtgc gtgcagcgta tgcagcacgg
    28741 tgaacagtgg gcgtggatga ttgtggttct ggcggcgatg ctggggcaaa ttggcctgcc
    28801 aggtggtggc tttggttttg gctggcacta taacggcgca ggcacgccgg ggcgtaaagg
    28861 cgttattctg agtggtttct ccggctctac gtcgattccg cctgttcacg acaacagtga
    28921 ttacaaaggc tacagcagca ccattccgat tgcccgtttt atcgatgcga tcctcgaacc
    28981 ggggaaagtg atcaactgga acggtaaatc ggtaaaactg ccgccgctga aaatgtgtat
    29041 ttttgccgga actaacccat tccatcgcca tcagcagatc aaccgcatta ttgaaggctg
    29101 gcgcaagctg gaaacggtta tcgccataga taaccagtgg acctcaacct gccgctttgc
    29161 cgatatcgtg ctgcctgcga ccacgcagtt tgagcgtaac gatctcgacc agtacggcaa
    29221 ccactccaac cgtggcatta tcgccatgaa acaggtggtg ccgccgcagt tcgaggcgcg
    29281 caacgacttt gatattttcc gcgagctgtg ccgtcgcttt aatcgcgaag aagcctttac
    29341 cgaagggctg gacgaaatgg gctggctgaa acgcatctgg caggaaggtg tacaacaagg
    29401 caaaggacgc ggcgttcatc tgccagcgtt tgatgacttc tggaataaca aagagtacgt
    29461 cgagtttgac catccgcaga tgtttgttcg ccaccaggca ttccgcgaag atccggatct
    29521 cgaaccgctg ggcacgccga gtggcctgat tgagatctac tcgaaaacca tcgccgatat
    29581 gaactacgac gattgtcagg ggcatccgat gtggtttgag aaaatcgaac gctcccacgg
    29641 tgggcccggc tcgcagaagt atccgttgca tctgcaatct gtgcatccgg atttccgact
    29701 tcactcgcag ttatgtgagt cggaaactct gcgtcagcaa tatacggtag cgggtaaaga
    29761 gccagtgttc attaacccgc aggatgccag cgcgcgcggt attcgtaacg gtgatgtggt
    29821 acgcgtcttt aacgctcgcg gtcaggtgtt ggcaggggca gtagtttctg accgctatgc
    29881 acccggcgtg gcacgaattc acgaaggggc atggtacgat ccagataaag gcggcgagcc
    29941 tggtgcgctg tgcaaatacg gtaaccccaa cgtgctgacc atcgacatcg gtacttcgca
    30001 gctggcgcag gcgaccagtg cgcacactac gctggtggaa attgagaagt acaatggagc
    30061 agtggagcag gtgacggcgt ttaacggccc tgtggagatg gtggcgcagt gcgaatatgt
    30121 tcccgcgtcg caggtgaaat catgaccacg ctgacagcac aacagattgc ctgtgtttac
    30181 gcctggctgg cgcagttgtt ctcccgtgaa ctggacgatg aacaactgac gcaaatcgcc
    30241 agtgcgcaga tggctgaatg gttttcgttg ctgaaaagcg aaccgccact ggctgcggcg
    30301 gtgaatgagc tggaaaactg tattgccacg ctgacagtac gtgacgatgc ccgtctggaa
    30361 ctggccgctg acttttgcgg cctgtttctg atgaccgaca aacaagcggc gctgccgtat
    30421 gcatcggcct acaaacagga cgagcaagag attaaacgct tgttagttga ggcagggatg
    30481 gaaaccagcg gcaatttcaa cgaaccggca gatcatctgg cgatctatct cgaattgctc
    30541 agccatctgc atttttcgct gggagagggg accgttcctg cgcgaagaat cgacagtttg
    30601 cggcaaaaaa cactgacggc gctgtggcaa tggttaccag agtttgttgt gcgttgtcgt
    30661 cagtatgaca gctttggttt ttacgcggca ctaagccagt tattgctggt gttagtggag
    30721 agcgaccacc aaaacagata acgtcgtttg tgcgcctgaa aagacgcgtt tagcgtcgca
    30781 tcaggcatta tggggcagtt gccggatgcg tcatcacatc cggcaaacac acatcacgga
    30841 tgagttacaa accgggaaag tcgctggcgc agcaggcggt tttcctgctt caggtgcgca
    30901 atatcatcca ttaacgtcag cgccaccgcg atcccaggcc agtccagagc cagttcatga
    30961 cgcaggcgta ctgcgcgttg caccacgatg gcggcatggt cgtcaaatac ccaggttgtt
    31021 tcctggatct cacgcggttc aaccaccccc aaaccgacaa tttcattcaa ctcctcttca
    31081 gagatgccgg tatgcaggca aaattcggta atagtaaaag tcaccgtaac attagccatt
    31141 atgctttccc ccaatcttta cgtggatcaa aagacgactg ggcgtctgcc agttgctgcc
    31201 acagcgcggc agtgttttca tccggtttcg gcggcatcac gattttcagt accgcataca
    31261 gatcgccggt ctgttttttg ctcaccagac ctttgccttt aacgcgcaat cgttgcccgg
    31321 cctggctgcc tggcgggata gtcagcaaaa tgctttcttt cagtgttgga acggtgactt
    31381 tagcacccag cgccgcttcc cacgggctaa ccggcactac aatttccaga tcatggccga
    31441 caatatcgaa cagcggatgt ggcgcaatat gaatcaccag ccacaaatcg ccatttggac
    31501 cgccgttttc gcccggcgtc ccctggcctt tcagacggat gcgttgacca ttgccgacgc
    31561 ccgccgggat cttgacattc agcgttttcg gaatttcctg ttcgatcatg ccaaaggcgt
    31621 tataaaccgg caggttatag ctgatggtac gcttatgctc agtaagcgtt tcttcgagga
    31681 ataccgccac ttcgatttca atatcgtggc cgcgtgtggc ggggcgttga cggctctggc
    31741 gggcatgctg accgaaaatt gacgagaaga tatcgtcaaa atcttcggcg ttaaaactct
    31801 gaccgtcgct atgatggaac tgacggttaa attgcggatc gttgcgatgt tgccacatct
    31861 gatcatactc agcgcgacgt tgttcatcac ttaacacttc ccaggcttca gcgacctctt
    31921 tgaagcgggc ttcggcatcc ggttctttgc tgacatcagg atggtatttg cgggccagtc
    31981 gacgataggc ggtcttgatt gtcttgagat cgtccgtcgg tttcacgccc atgatggcgt
    32041 aataatcctt taattccata gcgttatctc gcgtaaatca acacaaattg aaggaacccc
    32101 tgtaagctaa ctcctataag tgtagggtaa tcctcaaaat ttcatatgcc aacacagaat
    32161 atgttattga aatcatcgcg gagaggaggt cgcctcaagg tggggtgctg aacatatttt
    32221 aaacaggtga aaaagggtga gcgatttttg atagtggaac caggcacttt aagtttaact
    32281 ggggcgtcat tatttattaa
//
LOCUS       DAZGTR010000044        32208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGTR010000044 DAZGTR010000000
VERSION     DAZGTR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.044"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            84..383
                     /locus_tag="ACHLCY_002234"
     CDS             84..383
                     /locus_tag="ACHLCY_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369488.1"
                     /translation="MGNGNFWQLFLGKEPYGKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(430..1312)
                     /gene="rarD"
                     /locus_tag="ACHLCY_002235"
                     /pseudo
     CDS             complement(430..1312)
                     /gene="rarD"
                     /locus_tag="ACHLCY_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003828953.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
     gene            complement(1364..1831)
                     /gene="yigI"
                     /locus_tag="ACHLCY_002236"
     CDS             complement(1364..1831)
                     /gene="yigI"
                     /locus_tag="ACHLCY_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP0369489.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1996..2865
                     /gene="pldA"
                     /locus_tag="ACHLCY_002237"
     CDS             1996..2865
                     /gene="pldA"
                     /locus_tag="ACHLCY_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP0369490.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2998..4827
                     /gene="recQ"
                     /locus_tag="ACHLCY_002238"
     CDS             2998..4827
                     /gene="recQ"
                     /locus_tag="ACHLCY_002238"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP0369491.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQVNGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4891..5511
                     /gene="rhtC"
                     /locus_tag="ACHLCY_002239"
     CDS             4891..5511
                     /gene="rhtC"
                     /locus_tag="ACHLCY_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP0369492.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5573..6193)
                     /gene="rhtB"
                     /locus_tag="ACHLCY_002240"
     CDS             complement(5573..6193)
                     /gene="rhtB"
                     /locus_tag="ACHLCY_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP0369493.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6304..7326
                     /gene="pldB"
                     /locus_tag="ACHLCY_002241"
     CDS             6304..7326
                     /gene="pldB"
                     /locus_tag="ACHLCY_002241"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP0369494.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7334..8134
                     /gene="yigL"
                     /locus_tag="ACHLCY_002242"
     CDS             7334..8134
                     /gene="yigL"
                     /locus_tag="ACHLCY_002242"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP0369495.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8210..9109
                     /gene="bioP"
                     /locus_tag="ACHLCY_002243"
     CDS             8210..9109
                     /gene="bioP"
                     /locus_tag="ACHLCY_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP0369496.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8997..9950)
                     /gene="metR"
                     /locus_tag="ACHLCY_002244"
     CDS             complement(8997..9950)
                     /gene="metR"
                     /locus_tag="ACHLCY_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP0369497.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10068..12329
                     /gene="metE"
                     /locus_tag="ACHLCY_002245"
     CDS             10068..12329
                     /gene="metE"
                     /locus_tag="ACHLCY_002245"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP0369498.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12368..13183)
                     /gene="ysgA"
                     /locus_tag="ACHLCY_002246"
     CDS             complement(12368..13183)
                     /gene="ysgA"
                     /locus_tag="ACHLCY_002246"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP0369499.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNCGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13445..14206
                     /gene="udp"
                     /locus_tag="ACHLCY_002247"
     CDS             13445..14206
                     /gene="udp"
                     /locus_tag="ACHLCY_002247"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP0369500.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            14347..15774
                     /gene="rmuC"
                     /locus_tag="ACHLCY_002248"
     CDS             14347..15774
                     /gene="rmuC"
                     /locus_tag="ACHLCY_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP0369501.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15869..16624
                     /gene="ubiE"
                     /locus_tag="ACHLCY_002249"
     CDS             15869..16624
                     /gene="ubiE"
                     /locus_tag="ACHLCY_002249"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP0369502.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16638..17243
                     /gene="ubiJ"
                     /locus_tag="ACHLCY_002250"
     CDS             16638..17243
                     /gene="ubiJ"
                     /locus_tag="ACHLCY_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP0369503.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17240..18880
                     /gene="ubiB"
                     /locus_tag="ACHLCY_002251"
     CDS             17240..18880
                     /gene="ubiB"
                     /locus_tag="ACHLCY_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP0369504.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18959..19228
                     /gene="tatA"
                     /locus_tag="ACHLCY_002252"
     CDS             18959..19228
                     /gene="tatA"
                     /locus_tag="ACHLCY_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP0369505.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19232..19747
                     /gene="tatB"
                     /locus_tag="ACHLCY_002253"
     CDS             19232..19747
                     /gene="tatB"
                     /locus_tag="ACHLCY_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP0369506.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19750..20526
                     /gene="tatC"
                     /locus_tag="ACHLCY_002254"
     CDS             19750..20526
                     /gene="tatC"
                     /locus_tag="ACHLCY_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP0369507.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20568..21350
                     /gene="tatD"
                     /locus_tag="ACHLCY_002255"
     CDS             20568..21350
                     /gene="tatD"
                     /locus_tag="ACHLCY_002255"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP0369508.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21347..21835)
                     /gene="rfaH"
                     /locus_tag="ACHLCY_002256"
     CDS             complement(21347..21835)
                     /gene="rfaH"
                     /locus_tag="ACHLCY_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP0369509.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22002..23495
                     /gene="ubiD"
                     /locus_tag="ACHLCY_002257"
     CDS             22002..23495
                     /gene="ubiD"
                     /locus_tag="ACHLCY_002257"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP0369510.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLC
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23541..24242
                     /gene="fre"
                     /locus_tag="ACHLCY_002258"
     CDS             23541..24242
                     /gene="fre"
                     /locus_tag="ACHLCY_002258"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP0369511.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24434..25597)
                     /gene="fadA"
                     /locus_tag="ACHLCY_002259"
     CDS             complement(24434..25597)
                     /gene="fadA"
                     /locus_tag="ACHLCY_002259"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP0369512.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRRLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSTSDIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERF"
     gene            complement(25607..27796)
                     /gene="fadB"
                     /locus_tag="ACHLCY_002260"
     CDS             complement(25607..27796)
                     /gene="fadB"
                     /locus_tag="ACHLCY_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP0369513.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALKRPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27986..29317
                     /gene="pepQ"
                     /locus_tag="ACHLCY_002261"
     CDS             27986..29317
                     /gene="pepQ"
                     /locus_tag="ACHLCY_002261"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP0369514.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29317..29931
                     /gene="yigZ"
                     /locus_tag="ACHLCY_002262"
     CDS             29317..29931
                     /gene="yigZ"
                     /locus_tag="ACHLCY_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP0369515.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29970..31421
                     /gene="trkH"
                     /locus_tag="ACHLCY_002263"
     CDS             29970..31421
                     /gene="trkH"
                     /locus_tag="ACHLCY_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP0369516.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31433..31978
                     /gene="hemG"
                     /locus_tag="ACHLCY_002264"
     CDS             31433..31978
                     /gene="hemG"
                     /locus_tag="ACHLCY_002264"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP0369517.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7485 a         8166 c         8809 g         7748 t
ORIGIN      
        1 ttattgatgg ggtatatttt aattaaatat aattgaaatg tatttttatt ctataaacaa
       61 tatcttgggt gaatggattc tatatgggaa atggtaactt ttggcagttg ttcttgggta
      121 aagaacctta cggcaaaaaa tctgattttg ttaaggcatg gttattgtgg tgggctatta
      181 ccatccttgt tctatttatt ttaagcctga ctacgacatt aaatttaaga agtataggta
      241 tagggttaag ttttgggttt tgtcaaatcc ttggtagata tacgatgtct ctaggatgga
      301 gtaagtactg gggagttttg ggggtatttc ctgtaacaaa tattgttctt tttcttatct
      361 taattttttt caaaaaaaga taaataagtc agtgggtagg ctctttaaac ggcctaccca
      421 tgttaattct taactttttc tacgctgcgt ataaatcgca tccatcacaa aaattgccag
      481 cgccacccaa ataaaggcga aagtcaccat cttatcgtca cccggttttt caccataaaa
      541 cgtcacagcc agcaggaaca tcagcgtcgg gccaatgtac tggaaaaagc ctaacgttga
      601 gagacgcaag cgcgtggcag cggcggtaaa acacaacagc ggtacggtag tgacaatacc
      661 ggcggcgatc agcagtaaat tcagcgacat cgggttttgc cccatatggc tggttgagct
      721 gtcggcaata gcaaacaggt aaattgccgc cacgggcagc agccacatgg tttcgattaa
      781 catgccggtt tgcgcttcaa cggcaatctt cttgcgtacc agaccgtaga aggcaaaact
      841 aaatgccagt cccagcgcga taataggtag cgaaccaaaa gtccacagct ggactaacac
      901 gccacatatc gccagaatca ccgccagcca ttgcatccgg cggaatcgct cgccgaggaa
      961 aatcatcccc agcacaatgt tcaccagcgg gttaataaag taaccaaggc tcgcttccag
     1021 catatggtga ttgttcaccg cccagataaa cagtagccag ttgccaccaa tcagcacggc
     1081 agagactgcc agcataaaaa ttttctgtgg cgtctgaatc agcgttttta aataggacca
     1141 ctggcggcaa atgctgcacc accataaaga aaaacgacca gatcacgcga tgcgtcagga
     1201 tttcatcggc aggcacgtag taaatcaact tgaagtacgc tggcgctata ccccaaataa
     1261 aataagcggc aagagcgagt aatacgccct gccgcgtttg ttttgcatcc atcgggaata
     1321 ctcattttta attggtaaca gcagtttacc tgcttttatg tcttcaacct accatatagg
     1381 tggcggtggc actggcaata taaagctgtt cttcattgtg taattcaacg cgggcgacgg
     1441 cgactttatt gcctgcacgc aacaggctac tggtagcagt aaaacgctcg cccctgcctg
     1501 ggcgcagata atcaacgcga agatcaatgg tccccatccg cgatagccgc tggcgtagtt
     1561 catcttcact gatggtttcg tggcgggtta aggtacttcc cacgcacacc agacctgcgg
     1621 cgacatccag cgctgacgca ataaccccgc cgtgcaaaat gctttgcgcc cagttaccca
     1681 ccatcattgg ctgattttta aaggccagct gtgcgaactc tttttcgtaa cgctccagct
     1741 ccatccctaa cgcgcggtta aatggcatgt gataaacaaa catctcaccc actaatttca
     1801 gggcttgttc agcggtcagt acggcagaca tatcatcctt acacttcatt ggttaatgaa
     1861 atgttgattt tatgcttctt tgttgttggt ttctacttta ggaagggata actaacggct
     1921 atggagttaa gtatgtaaaa tagcccgcag aaaaatattc accttatcaa taattcgtta
     1981 cggagaacac gaccgatgcg gactctgcag ggctggttgt tgccggtgtt tatgttgcct
     2041 atggcagtat atgcacaaga ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt
     2101 ggcagtatta tcgccaatat gctgcaggag catgacaatc cgttcacgct ctatccttat
     2161 gacaccaact acctcattta cacccaaacc agcgatctga ataaagaagc gattgccagt
     2221 tacgactggg cggaaaatgc gcgtaaggat gaagtaaagt ttcagttgag cctggcattt
     2281 ccgctgtggc gtgggatttt aggcccgaac tcggtgttgg gtgcgtctta tacgcaaaaa
     2341 tcctggtggc aactgtccaa tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa
     2401 ccgcaattgt tcctcggttt tgccaccgat taccgttttg caggttggac gctgcgcgat
     2461 gtggagatgg ggtataacca cgactctaac gggcgttccg acccgacctc ccgcagctgg
     2521 aaccgccttt atactcgcct gatggcagaa aacggtaact ggctggtaga agtgaagccg
     2581 tggtatgtgg tgggtaatac tgacgataac ccggatatca ccaaatatat gggttactac
     2641 cagcttaaaa tcggctatca cctcggtgat gcggtgctca gtgcgaaagg acagtacaac
     2701 tggaacaccg gctacggcgg cgcggagtta ggcttaagtt acccgatcac caaacatgtg
     2761 cgcctttata ctcaggttta cagcggctat ggcgaatcgc tcatcgacta taacttcaac
     2821 cagacccgtg tcggtgtggg ggttatgcta aacgatttgt tttgatgaac ggttgagtgg
     2881 ttggcaaatc tggaacccag catccaggat taccctctca gagactaaaa gcattgcagt
     2941 ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg
     3001 gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac aggttttaca agaaaccttt
     3061 ggctaccaac agtttcgccc cggccaggaa gaaattatcg acactgtgct ttccggtcgc
     3121 gattgcctgg tcgtcatgcc caccggtggc ggaaaatccc tttgctatca aattcctgcc
     3181 ttattgctaa acggccttac cgtggttgtt tcaccgctga tttcgttgat gaaagatcag
     3241 gtggatcaac tgcaagtcaa cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc
     3301 gaacagcaac ttgaagtgat gacaggctgc cgcaccgggc aaattcgtct gctttatatc
     3361 gccccggaac gcctgatgct ggataacttt cttgagcatc tggcgcactg gaatccggtg
     3421 ttattagccg ttgatgaagc gcactgtatc tcccaatggg gccacgattt ccgcccggaa
     3481 tatgccgcgc tcggtcagtt gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc
     3541 gccacagccg acgacaccac gcgccaggat atcgtgcgcc tgctggggct gaacgatccg
     3601 ctgattcaaa tcagcagttt tgaccgtccg aatattcgct acatgctgat ggagaagttc
     3661 aaaccgctcg atcagttgat gcgctacgtg caggaacagc gcggtaagtc cggcattatc
     3721 tactgcaaca gccgggcgaa agtagaagac accgctgcgc gcctgcaaag caagggtatt
     3781 agcgcggcgg cctatcatgc cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa
     3841 ttccagcgcg atgacctgca aattgtggtg gcgacggtgg cgttcggcat gggcatcaat
     3901 aaaccaaacg ttcgcttcgt ggtccacttt gatattccgc gcaatatcga atcctattat
     3961 caggaaaccg gtcgcgccgg gcgtgatggc ctgcccgcgg aagcgatgct gttttacgat
     4021 ccggccgata tggcgtggct gcgccgttgt ctggaagaga agccgcaggg gcagttgcag
     4081 gatatcgaac gtcataaact caatgcgatg ggcgcttttg ccgaagcgca aacttgccgt
     4141 cgtctggtac tgctgaacta ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat
     4201 atctgcctcg atccgccgaa acagtacgac ggttcaaccg atgctcagat tgccctttcc
     4261 accattggtc gtgtgaatca gcgttttggt atgggctatg tggtggaagt gattcgcggc
     4321 gctaataacc agcgtatccg cgactatggt catgacaaac taaaagtcta cggtatgggc
     4381 cgtgataaaa gtcatgaaca ttgggtgagc gtgatccgcc agctgattca cctcggtctg
     4441 gtgacgcaaa atattgccca gcattctgcc ctacaactga cagaggctgc gcgtccggtg
     4501 ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa
     4561 gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac tgttcgccaa attacgcaaa
     4621 ctgcgtaaat cgatagccga tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca
     4681 accttgattg agatggctga acagatgccg atcaccgcca gcgaaatgct cagcgttaac
     4741 ggcgttggga tgcgcaagct ggaacgcttt ggcaaaccgt ttatggcgct gattcgtgcg
     4801 catgttgatg gcgatgacga agagtagtca gcagcataaa aaagtgccag tatgaagact
     4861 ccgtaaacgt ttcccccgcg agtcaaatgt atgttgatgt tatttctcac cgtcgccatg
     4921 gtgcacattg tggcgcttat gagccccggt cccgatttct tttttgtctc tcagaccgct
     4981 gtcagtcgtt cccgtaaaga agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg
     5041 gtttgggctg ggattgcgct gcttggcctg catttgatta tcgaaaaaat ggcctggctg
     5101 catacgctga ttatggtggg cggtggcctg tatctctgct ggatgggtta ccagatgcta
     5161 cgcggtgcac tgaaaaaaga ggcggtttct gcacctgcgc cacaggtcga gctggcgaaa
     5221 agtgggcgca gtttcctgaa aggtttactg accaatctcg ctaatccgaa agcgattatc
     5281 tactttggct cggtgttctc attgtttgtc ggtgataacg ttggcactac cgcgcgctgg
     5341 ggcatttttg cgctgatcat tgtcgaaacg ctggcgtggt ttaccgtcgt tgccagcctg
     5401 tttgccctgc cgcaaatgcg ccgtggttat caacgtctgg cgaagtggat tgatggtttt
     5461 gccggggcgt tatttgccgg atttggcatt catttgatta tttcgcggtg atgccagacg
     5521 cgtcttcaga gtaagtcgga taaggcgttt acgccgcatc cgacattatt tttcacgcat
     5581 gcctcgccga tgctaacagc gctcccacca gcataaacaa cgagccgaaa atcttattca
     5641 gcgccttcat ctgctttggt cctttaatcc atagagcaat ccgttgagca agggtggcgt
     5701 aaccgatcat cacaataata tcgaccacaa tagtggtgac gccgagcacg atatactgca
     5761 tcagttgcgg ctgttgcggc atgatgaatt gcggaaatag cgccgccaga aacacaatac
     5821 ttttgggatt ggtgagattc acaaaaactg cgcgctggaa caaatgtcga cgcgattgag
     5881 tagaggccag cgatttaagg tcaattgcac cagcggcgcg ccactgctgg attcccagcc
     5941 aaatcaagta agccgcgcct gcccacttca acacttcaaa cgcaatcact gagcgggaaa
     6001 atagcgtccc caaccccacg ccaaccagca caatatgaat cgccagtccg gtctgaagcc
     6061 cagcaataga cgccaccgcg ccgcgataac cgtggttgag cgaggtggtc atagtgttga
     6121 ttgcaccaga gcctggcgac aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc
     6181 attctaaggt catgatgaac tcccggtgtg tctatttttg tgccacaata cgctactgtc
     6241 gcagcgttgt gtcaggcacg ctaaaaaaaa cgattttacg tggtttaaga ggcagattac
     6301 ccgatgtttc agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc
     6361 atgggaccgc tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat
     6421 gacattccgg tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc
     6481 tgcccggggc gtattgagag ctacgtaaaa tatgcggaac tggcctatga cctgttccat
     6541 ttggggtttg atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta
     6601 gccgatccgc atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca
     6661 ttctggcagc aggaggttca gcccggtccg tggcgtaaac gctatatact ggcacattcg
     6721 atgggcggtg cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt
     6781 gcgctaactg cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag
     6841 atcctcaact gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg
     6901 cgctggcgcg cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga
     6961 cgtaacttac gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat
     7021 tgggtacgcg aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc
     7081 acgccaacgc ttctcttaca agctgaagag gaacgcgtgg tggataaccg catgcatgac
     7141 cgtttttgtg aactccgcac cgccgcgggc catcctgtcg aaggaggacg gccgttggta
     7201 attaaaggtg cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc
     7261 cacgccatcg ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag
     7321 gtttaaattt cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc
     7381 cgaccatacg ttatcccctt acgccaaaga aactctgaag ctgctcaccg cgcgcggcat
     7441 caactttgtg tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct
     7501 ggagattaag tcttacatga ttacctccaa tggtgcgcgc gttcacgatc tggatggtaa
     7561 tctgattttt gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa
     7621 cgacaatccg gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca
     7681 tcgcccggaa gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc
     7741 tggattactg gagccggaag gcgtcagcaa agtgtttttc acctgcgatt cccatgaaca
     7801 actgctgccg ctggagcagg cgattaacgc tcgctggggc gatcgcgtca acgtcagttt
     7861 ctctacctta acctgtctgg aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga
     7921 agcagtggcg aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat
     7981 gaacgacgcc gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca
     8041 gcgtctgaaa gaccttcatc ccgagctgga agtgattggt actaatgccg acgacgcggt
     8101 gccgcattat ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa
     8161 cctgatactt cgctacaatg gatactcgtt aatcaaagag ttttccattg tggcgctact
     8221 tatcatcacc acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg
     8281 gcacgtcgat agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct
     8341 gccgtttctg cgtacccgtg gcaatagcct gaaaacggtc ggcctgtata tgttggtggg
     8401 cgcgatgcag ctcggcgtga tgtatatgct gagtttccgc gcgtatctct acctgacggt
     8461 ctccgaactg ctgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat
     8521 catgagtaag cgccgtctgc gctggggcta tgcctttagc gccttgttgg cggtgattgg
     8581 tgccgggatt attcgctatg atcaggtcac cgaccatttc tggactggct tgctgctggt
     8641 gcaactctcc aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac
     8701 tcgcccgatg ccacagcata acgcctttgc gtggttctat cttggcgcgt ttctggtggc
     8761 ggtaattgca tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg
     8821 gggcattctg gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta
     8881 cggcgcgacg caggtggacg ccggaacgct gggcattatg aataatatgc acgttccggc
     8941 agggctgctg gtaaacctgg ctatctggca ccaacagccg cactggccaa cgttcattac
     9001 aggcgcgctg gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc
     9061 ttcacaaacg gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa tgcttctgta
     9121 atcggctgac gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg
     9181 cccagggttt tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt
     9241 agcgcggcaa tacccatccg cgcggcaacc atctgaatca acaataaggt gttatcgacg
     9301 cttttcagtg acgggctgac gcctgccggc tgaagaaaat gccgccagac atccagtcga
     9361 ctacgctgca ccggataaat caacagcgtc tcgctggcga gatcttccgg tgtaattcgc
     9421 gttttcgccg ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc
     9481 ggcgaataat gcaggccact gcgcggcagg atatcggagg tcatcaccag atccagctcg
     9541 ccctgttgca acgcgggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc
     9601 tgcggccagt tcttatggaa attttctaac gcgggcgtca gccactgaat acagctatgg
     9661 cactcaatgg caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg
     9721 ctaatttgcg gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag
     9781 cgtagcggct ggctcttacg cacaaatagc cggaaaccaa ggcgttgttc cagatcgcta
     9841 aactggtgag acagggcgga ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag
     9901 ccgcagttcc gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt
     9961 cacttcggca tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg
    10021 taaacatctg gacggctaaa tactaaaaat tccataaggg gcatataatg acaattctta
    10081 atcacaccct cggtttccct cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa
    10141 gttattgggc ggggaactcc acgcgtgaag aactgctgac agtagggcgt gaactgcgtg
    10201 ctcgtcactg ggatcaacaa aagcaagcgg gtatcgacct gctgccggtg ggcgattttg
    10261 cctggtacga tcatgttctg accaccagtc tgctgctggg taatgttccg ccacgtcatc
    10321 agaacaaaga cggttcggta gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc
    10381 cgactggcga acctgcggcg gcagcggaaa tgaccaaatg gtttaacacc aactatcact
    10441 acatggtgcc ggaattcgtt aaaggccaac agttcaaact gacctggacg cagctgctgg
    10501 aggaagtgga cgaggcgctg gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg
    10561 ttacttacct gtggctgggg aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga
    10621 acgacattct gccggtttat cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt
    10681 gggtacagat tgatgaaccc gcgctggtac tggaactacc acaggcgtgg ctggacgcat
    10741 acaaacccgc ttacgacgcg ctccagggac aggtgaaact gctgctgacc acctattttg
    10801 aaggcgtaac gccaaatctc gacacgatta ctgcgctgcc tgttcagggt ctgcatgttg
    10861 acctcgtaca tggtaaagat gacgttgctg aactgcacaa gcgcctgcct tctgactggt
    10921 tgctgtctgc gggtctgatc aatggtcgta acgtctggcg cgccgatctt accgagaaat
    10981 atgcgcaaat taaggacatt gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt
    11041 tgctgcacag ccccatcgac ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct
    11101 ggtttgcctt cgccctacaa aaatgccatg aactggcact gctgcgcgat gcgctgaaca
    11161 gtggtgacac ggcagctctg gcagagtgga gcgccccgat tcaggcacgt cgtcactcta
    11221 cccgcgtaca taatccggcg gtagaaaagc gtctggcggc gatcaccgcc caggacagcc
    11281 agcgtgcgaa tgtctatgaa gtgcgtgccg aagcccagcg tgcgcgtttt aaactgccag
    11341 cgtggccgac caccacgatt ggttctttcc cgcaaaccac ggaaattcgt accctgcgtc
    11401 tggatttcaa aaaaggcaat ctcgacgcca ataactaccg cacgggcatt gcggaacata
    11461 tcaggcaggc cattgttgaa caggaacgtt tgggactgga tgtgctggta catggcgagg
    11521 ccgagcgtaa tgacatggtg gaatactttg gcgagcacct cgacgggttt gtctttacgc
    11581 aaaacggttg ggtacagagc tacggttccc gctgcgtgaa gccaccgatt gttattggtg
    11641 acgttagtcg cccggcaccg attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg
    11701 acaaaccggt gaaagggatg ctgacggggc cggtgaccat actctgctgg tcgttcccgc
    11761 gtgaagatgt cagccgtgaa accatcgcca aacagattgc gctggcgctg cgtgatgagg
    11821 tggccgatct ggaagcggca gggattggta tcatccagat tgacgaaccg gcgctgcgcg
    11881 aaggtttacc actgcgtcgt agcgactggg acgcctatct ccagtggggc gtagaggcct
    11941 tccgtatcaa cgccgccgtg gcgaaagatg acacacaaat ccacactcac atgtgttatt
    12001 gcgagttcaa cgacatcatg gattccattg cggcgctgga tgcagacgtc atcaccatcg
    12061 aaacctcgcg ttccgacatg gagttgctgg agtcgtttga agagtttgat tatccgaatg
    12121 aaatcggtcc aggcgtctat gacattcact cgccaaacgt accgagcgtg gaatggattg
    12181 aagccctgct gaagaaagcg gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccag
    12241 actgtggcct gaaaacgcgc ggctggccag aaacccgcgc ggcactggcg aacatggtgc
    12301 aggcggcgca gaatttgcgt ctggggtaaa tccaaaccgg gtggtaatac cacccggtct
    12361 tttctcatta cagcgacttc ttcccaccat actgcttaaa ccattccagc atacgctgcc
    12421 agccatcttt cgcagattcg gcatgatagc tcgggcgata atcagcgttg aatgcatgcc
    12481 cggcatccgg gtagacgata atctctgctt tcgcattagc agcccgcagc gcctggcgca
    12541 tcgtttcaac gctctcctgc ggaatgctgt tatcctgacc gccatataag ccgagaaccg
    12601 gcgcgttaag atcggttgcg atatcaacag gttgtttcgg tgaattcagc gacttatcgc
    12661 ccgtcagttt gccgtaccac gccactgcgg cttttagctg tggattatgc gcggcataca
    12721 gccaggtgat acgtccaccc cagcagaatc cggtgatcat taaacgatga acatcgccgc
    12781 agttgcggga agcccaactg gcgacatgat cgagatcggc cagcacctgc gagtcaggca
    12841 cttttgctac cagaccgcta agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt
    12901 cgcggaagta aagttcaggt gcgatagcca gatacccctc cagcgccaga cggcgacaaa
    12961 tgtcgcggat atgttcatgc acgccaaaaa tttcctgcac tacaatgacc actggcagtg
    13021 ggccatcgct ttgctttggt ctggcatggt aagcaggcat gttatcccct tgtgaaggga
    13081 tagaggtgaa gcccgccaca atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag
    13141 gcgatgcagc aggtgcaaat ccagattgtt gtgttgttgc catggtattc tccgtaccct
    13201 tataaaaatg ttgcgcaatg ttaactatag tcagcatgca acaaatcaca ttgcctgaat
    13261 cggctcatct tttatgcagt cctgcagaat gaagagtgat ttatgtgatt tgcatcactt
    13321 ttggtgggta aatttatgca acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt
    13381 taaacccttc ggtaaagtgt ctttttgctt cttctgacta aaccgattca cagaggagtt
    13441 gtatatgtcc aagtctgatg tttttcatct cggcctcact aaaaacgatt tacaaggggc
    13501 tacgcttgcc atcgtccctg gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga
    13561 taagccggtt aagctggcat ctcaccgcga attcaccacc tggcgtgcag agctggatgg
    13621 taaacctgtt atcgtctgct ctaccggtat cggcggcccg tctacctcta ttgctgttga
    13681 agagctggca cagctgggca ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca
    13741 gccgcatatt aatgtgggtg atgtcctggt taccacggcg tctgtccgtc tggatggcgc
    13801 gagcctgcac ttcgcaccgc tggaattccc ggctgtcgct gatttcgaat gtacgactgc
    13861 gctggttgaa gctgcgaaat ccattggcgc gacaactcac gttggcgtga cagcttcttc
    13921 tgataccttc tacccaggtc aggaacgtta cgatacttac tctggtcgcg tagttcgtca
    13981 ctttaaaggt tctatggaag agtggcaggc gatgggcgta atgaactatg aaatggaatc
    14041 tgcaaccctg ctgaccatgt gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt
    14101 tatcgttaac cgcacccagc aagagatccc gaatgctgag acgatgaaac aaaccgaaag
    14161 ccatgcggtg aaaatcgtgg tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt
    14221 ctgaaggccg acgcgttcgg ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt
    14281 tccaactgga cgtttgtaca gcacaattct attttgtgcg ggtaagttgt tgcgtcagga
    14341 ggcgttgtgg atttctcaat catggtttac gcagttattg cgttggtggg tgtggcaatt
    14401 ggctggctgt ttgccagtta tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt
    14461 gaagagatgg tcgcggagtt aagcgcggca aaacaacaaa ttacccaaag cgagcactgg
    14521 cgtgcagagt gcgagttact caataacgaa gtgcgcagcc tgcaaagtat taacacctct
    14581 ctggaggccg atctgcgtga agtaaccacg cggatggaag ccgcacagca acatgctgac
    14641 gataaaattc gccagatgat taacagcgag cagcgcctca gtgagcagtt tgaaaacctc
    14701 gccaaccgta tttttgagca cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg
    14761 aacagcctgt tgtcgccgct acgtgaacaa ctggacggtt tccgccgtca ggttcaggac
    14821 agcttcggta aagaagcaca agaacgccat accctgaccc acgaaattcg caatctccag
    14881 caactcaacg cgcaaatggc ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac
    14941 aataaaaccc agggcaactg gggcgaggta gtattgacgc gggtgctgga ggcttccggt
    15001 ctgcgtgaag ggtatgaata tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg
    15061 atgcagccgg atgtcatcgt gcggctgccg cagggaaaag atgtggtgat cgacgccaaa
    15121 atgacgctgg tcgcctatga acgctatttt aacgccgaag acgactacac ccgcgaaagc
    15181 gcgctacagg aacatatcgc gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat
    15241 tatcaacagc tgccggggct gcgaactctg gattacgtgc tgatgtttat tcccgttgaa
    15301 cccgcttttt cactggcgct tgaccgccag ccggagctga tcaccgaagc gttgaaaaac
    15361 aatatcatgc tggttagccc gactacgctg ctggtggcgt tgcgtactat tgccaacctg
    15421 tggcgttatg agcatcaaag ccgcaacgcc cagcaaatcg ccgatcgtgc cagcaagctg
    15481 tacgacaaga tgcgtttgtt catcgatgac atgtccgcga ttggtcaaag tctcgacaaa
    15541 gcgcaggata actaccgcca ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg
    15601 gcgcaggcag aagcgtttcg cggtttagga gtagaaatta aacgcgagat taatccggat
    15661 ttggctgaac aggcggtgag ccaggatgaa gagtatcgac ttcggtcggt tccggagcag
    15721 ccgaatgatg aagcttatca acgcgatgat gaatataatc agcagtcgcg ctagcccatt
    15781 aggggtagtt aagccgggta gaaatctagg gcatcgacgc ccaatctgtt acacttctgg
    15841 aacaattttt tgatgagcag gcattgagat ggtggataag tcacaagaaa cgacgcactt
    15901 tggttttcag accgtcgcga aggaacaaaa agcggatatg gtcgcccacg ttttccattc
    15961 cgtggcatca aaatacgatg tcatgaatga tttgatgtca tttggtattc atcgtttgtg
    16021 gaagcgattc acgattgatt gcagcggcgt acgccgtggg cagaccgtgc tggatctggc
    16081 tggtggcacc ggcgacctga cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt
    16141 ggtccttgct gatatcaatg aatccatgct caaaatggga cgcgagaagc tgcgtaatat
    16201 cggtgtgatt ggcaacgttg agtatgttca ggcgaacgct gaggcgctgc cgttcccgga
    16261 taacaccttt gattgcatca ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa
    16321 agcactgcgt tcaatgtatc gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt
    16381 ctcgaagcca attatcgagc cgctgagcaa agcctatgat gcatactcct tccatgtgct
    16441 gccgcgtatt ggctcactgg tcgcgaacga cgccgacagc taccgttatc tggcagaatc
    16501 catccgtatg catcccgatc aggataccct gaaagccatg atgcaggatg ccggattcga
    16561 aagtgtcgac tactacaatc tgacggcggg ggttgtggcg ctgcatcgtg gttataagtt
    16621 ctgacaggag accggaaatg ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc
    16681 tcaacacctt cctgtatcgc tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta
    16741 aagtattgcg cgtggaggta aaaggctttt cgacgtcatt gattctggtg ttcagcgaac
    16801 gccaggttga tgtactgggc gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg
    16861 ccagtgtgtt gccgaaactt cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg
    16921 agctggaagt gcagggcgat attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg
    16981 cagagttcga ccctgcggaa ctgctggccc cttataccgg tgatatcgcc gctgaaggaa
    17041 tcagcaaagc catgcgcgga ggcgcaaagt tcctgcatca cggcattaag cgccagcaac
    17101 gttatgtggc ggaagccatt actgaagagt ggcgtatggc acccggtccg cttgaagtgg
    17161 cctggtttgc ggaagagacg gctgccgtcg agcgtgctgt tgatgccctg accaaacggc
    17221 tggaaaaact ggaggctaaa tgacgccagg tgaagtacgg cgcctatatt tcatcattcg
    17281 cactttttta agctacggac ttgatgaact gatccccaaa atgcgtatca ccctgccgct
    17341 acggctatgg cgatactcat tattctggat gccaaatcgg cataaagaca aacttttagg
    17401 tgagcgacta cgactggccc tgcaagaact ggggccggtt tggatcaagt tcgggcaaat
    17461 gttatcaacc cgccgcgatc tttttccacc gcatattgcc gatcagctgg cgttattgca
    17521 ggacaaagtt gctccgtttg atggcaagct ggcgaagcag cagattgaag ctgcaatggg
    17581 cggcttgccg gtagaagcgt ggtttgacga ttttgaaatc aagccgctgg cttctgcttc
    17641 tatcgcccag gttcataccg cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt
    17701 catccgcccg gatattttgc cggtcattaa agcggatctg aaacttatct accgtctggc
    17761 tcgctgggtg ccgcgtttgc tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg
    17821 cgagtacgaa aagaccttga ttgatgaact gaatttgctg cgggaatctg ccaacgccat
    17881 tcagcttcgg cgcaattttg aagacagccc gatgctctac atcccggaag tttaccctga
    17941 ctattgtagt gaagggatga tggtgatgga gcgcatttac ggcattccgg tgtctgatgt
    18001 tgcggcgctg gagaaaaacg gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt
    18061 gttcttcact caggtctttc gcgacagctt tttccatgcc gatatgcacc ctggcaacat
    18121 cttcgtaagc tatgaacacc cggaaaaccc gaaatatatc ggcattgatt gcgggattgt
    18181 tggctcgcta aacaaagaag ataaacgcta tctggcagaa aactttatcg ccttctttaa
    18241 tcgcgactat cgcaaagtgg cagagctaca cgtcgattca ggctgggtgc caccagatac
    18301 caacgttgaa gagttcgaat ttgccattcg tacggtctgt gaacctatct ttgagaaacc
    18361 gctggccgaa atttcgtttg gacatgtact gttaaatctg tttaatacgg cgcgtcgctt
    18421 caatatggaa gtgcagccgc aactggtgtt actccagaaa accctgctct atgtcgaagg
    18481 ggtaggacgc cagctttatc cgcagctcga tttatggaaa acagcgaagc ctttcctgga
    18541 gtcgtggatt aaagatcagg tcggtattcc tgcgctggtg agagcattta aagaaaaagc
    18601 gccgttctgg gtcgaaaaaa tgccagaact gcctgaattg gtttacgaca gtttgcgcca
    18661 gggcaagtat ttacagcaca gtgttgataa gattgcccgc gagcttcagt caaatcatgt
    18721 acgtcaggga caatcgcgtt attttctcgg aattggcgct acgttagtat taagtggcac
    18781 attcttgttg gtcagccgac ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg
    18841 tctgatcgcc tggtttgtcg gttggcgcaa aacacgctga ttttttcatc gctcaaggcg
    18901 ggccgtgtaa cgtataatgc ggctttgttt aatcatcatc taccacagag gaacatgtat
    18961 gggtggtatc agtatttggc agttattgat tattgccgtc atcgttgtac tgctttttgg
    19021 caccaaaaag ctcggctcca tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa
    19081 agcaatgagc gatgatgaac caaagcagga taaaaccagt caggatgctg attttactgc
    19141 gaaaactatc gccgataagc aggcggatac gaatcaggaa caggctaaaa cagaagacgc
    19201 gaagcgccac gataaagagc aggtgtaatc cgtgtttgat atcggtttta gcgaactgct
    19261 attggtgttc atcatcggcc tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa
    19321 aacggtagcg ggctggattc gcgcgttgcg ttcactggcg acaacggtgc agaacgaact
    19381 gacccaggag ttaaaactcc aggagtttca ggacagtctg aaaaaggttg aaaaggcgag
    19441 cctcactaac ctgacgcccg aactgaaagc gtcgatggat gaactacgcc aggccgcgga
    19501 gtcgatgaag cgttcctacg ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac
    19561 catccataac ccggtggtga aagataatga agctgcgcat gagggcgtaa cgcctgccgc
    19621 tgcacaaacg caggccagtt cgccggaaca gaagccagaa accacgccag agccggtggt
    19681 aaaacctgct gcggacgctg aaccgaaaac cgctgcacct tccccttcgt cgagtgataa
    19741 accgtaaaca tgtctgtaga agatactcaa ccgcttatca cgcatctgat tgagctgcgt
    19801 aagcgtctgc tgaactgcat tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc
    19861 gccaatgata tctatcacct ggtatccgcg ccattgatca agcagttgcc gcaaggttca
    19921 acgatgatcg ccaccgacgt ggcctcgccg ttctttacgc cgatcaagct gacctttatg
    19981 gtgtcgctga ttctgtcagc gccggtgatt ctctatcagg tgtgggcatt tatcgcccca
    20041 gcgctgtata agcatgaacg tcgcctggtg gtgccgctgc tggtttccag ctctctgctg
    20101 ttttatatcg gtatggcatt cgcctacttt gtggtctttc cgctggcatt tggcttcctt
    20161 gccaataccg cgccggaagg ggtgcaggta tccaccgaca tcgccagcta tttaagcttc
    20221 gttatggcgc tgtttatggc gtttggtgtc tcctttgaag tgccggtggc aattgtgctg
    20281 ctgtgctgga tggggattac ctcgccagaa gacttacgca aaaaacgccc gtatgtgctg
    20341 gttggtgcat tcgttgtcgg gatgttgctg acgccgccgg atgtcttctc gcaaacgcta
    20401 ttggcgatcc cgatgtactg tctgtttgaa atcggtgtct tcttctcacg cttttacgtt
    20461 ggtaaagggc gaaatcggga agaggaaaac gacgctgaag cagaaagcga aaaaactgaa
    20521 gaataaattc aaccgcccgt cagggcggtt gtcatatgga gtacaggatg tttgatatcg
    20581 gcgttaattt gaccagttcg caatttgcga aagaccgtga tgatgttgta gcgcgcgctt
    20641 ttgacgcggg agttaatggg ctactcatca ccggcactaa cctgcgtgaa agccagcagg
    20701 cgcaaaagct ggcgcgtcag tattcgtcct gttggtcaac ggcgggcgta catcctcacg
    20761 acagcagcca gtggcaagct gcgactgaag aagcgattat tgagctggcc gcgcagccag
    20821 aagtggtggc gattggtgaa tgtggtctcg actttaaccg caacttttcg acgccggaag
    20881 agcaggaacg cgcttttgtt gctcagctac gcattgccgc agaattaaac atgccggtat
    20941 ttatgcactg tcgcgatgcc cacgagcggt ttatgacatt gctggagccg tggctggata
    21001 aactgcctgg tgcggttctt cattgcttta ccggcacacg cgaagagatg caggcgtgcg
    21061 tggcgcgtgg aatttatatc ggcattaccg gttgggtttg cgatgaacga cgcgggctgg
    21121 agctgcggga attgttgccg ttgattccgg cggagaaatt gctgatcgaa actgatgcgc
    21181 cgtatctgct cccgcgcgat ctcacgccaa agccatcatc ccggcgcaac gagccagccc
    21241 atctgcccca tattttgcaa cgtattgcgc actggcgtgg agaagatgcc gcatggctgg
    21301 ctgccaccac ggatgctaat gtcaaaacac tgtttgggat tgcgttttag agtttgcgga
    21361 actcggtgtt cttcacactg tgcttaatct ctttattaat aagattaagc aatagcatgg
    21421 agcgagcctc accatcgggt tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg
    21481 taataatcac cttatcaccc ggataagggg ttgccggatc gacaatgtct ttcggtttat
    21541 ataccgatag ctgatgaatc accgccgatg ggactatcgc tggcgacgcg ccaaagcgca
    21601 cgaagtggct gacaccgcgg gtcgcgttga tagtcgtggt atgaatcact tctggatcaa
    21661 attcgacaaa caggtagttg ggaaacaatg gctcactgac tgcagtacgt tttccacgca
    21721 cgattttttc cagggtgatc atcggtgcca ggcaattcac agcctgtctt tcgaggtgtt
    21781 cctgggcacg ttgaagttgc ccgcgcttgc agtacagtaa ataccaggat tgcataatga
    21841 ctcttatccg tttaatcggg gcgcaaggat agcaaaagct ttacgctaag ttaattatat
    21901 tccccggttt gcgttatacc gtcagagttc acgctaattt aacaaattta cagcatcgca
    21961 aagatgaacg ccgtataatg ggcgtagatt aagaggctac aatggacgcc atgaaatata
    22021 acgatttacg cgacttcttg acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc
    22081 tcccggtgga tccgcatctg gaaatcactg aaattgctga ccgcactttg tgtgccggtg
    22141 ggcctgcgct gttgttcgaa aaccctaaag gctactcaat gccggtgctg tgcaacctgt
    22201 tcggtacgcc aaagcgcgtg gcgatgggca tggggcagga agatgtttcg gcgctgcgtg
    22261 aagttggtaa attattggcg tttctgaaag agccggagcc gccaaaaggt ttccgcgacc
    22321 tgtttgataa actgccgcag tttaagcaag tattgaacat gccgacaaag cggctgcgtg
    22381 gtgcgccctg ccaacaaaaa atcgtctctg gcgatgacgt cgatctcaat cgcattccca
    22441 ttatgacctg ctggccggaa gatgccgcgc cgctgattac ctgggggctg acagtgacgc
    22501 gcggcccgca taaagagcgg cagaatctgg gcatttatcg ccagcagctg attggtaaaa
    22561 acaaactgat tatgcgctgg ctgtcgcatc gcggcggcgc gctggattat caggagtggt
    22621 gtgcggcgca tccgggcgaa cgtttcccgg tttctgtggc gctgggtgcc gatcccgcca
    22681 cgattctcgg tgcagtcacc cccgttccgg atacactttc agagtatgcg tttgccggat
    22741 tgctgcgcgg taccaagacc gaagtggtga agtgtatctc caatgacctt gaagtgcccg
    22801 ccagtgcgga gattgtgctg gaagggtata tcgaacaagg tgaaacggcg ccggaagggc
    22861 cgtatggcga ccacaccggt tactataacg aagtcgatag tttcccggta tttaccgtga
    22921 cgcatattac ccagcgtgaa gatgcgattt accattccac ctataccggg cgtccgccag
    22981 atgagcccgc ggtactgggc gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac
    23041 agttcccgga aattgtcgat ttttacctgc cgccggaagg ctgttcttat cgtctggcgg
    23101 tagtgacgat caaaaaacag tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt
    23161 cgttcttacg ccagtttatg tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg
    23221 cacgcgactg gaacgatgtg atttgggcga ttaccacccg tatggacccg gcgcgggata
    23281 ctgttctggt agaaaatacg cctattgatt atctggattt tgcctcgcct gtctccgggc
    23341 tgggttcaaa aatggggctg gatgccacga ataaatggcc gggggaaacc cagcgtgaat
    23401 ggggacgtcc catcaaaaaa gatccagatg ttgtcgcgca tattgacgcc atctgggatg
    23461 aactggctat ttttaacaac ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat
    23521 cgatccgaca gagaaagcgc atgacaacct taagctgtaa agtgacctcg gtagaagcta
    23581 tcacggatac cgtatatcgt gtccgcatcg tgccagacgc ggccttttct tttcgtgctg
    23641 gtcagtattt gatggtagtg atggatgagc gcgacaaacg tccgttctca atggcctcga
    23701 cgccggatga aaaagggttt atcgagctgc atattggcgc ttctgaaatc aacctttacg
    23761 cgaaagcagt catggaccgc atcctcaaag atcatcaaat cgtggtcgat attccccacg
    23821 gagaagcgtg gctgcgcgat gatgaagagc gtccgatgat tttgattgcg ggcggcaccg
    23881 ggttctctta tgcccgctcg attttgctga cagcgttggc gcgtaaccca aaccgtgata
    23941 tcaccattta ctggggcggg cgtgaagagc agcatctgta tgatctctgc gagcttgagg
    24001 cgctttcgtt aaagcaccct ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag
    24061 gctggcgtgg gcgtactggt accgtgttaa cggcggtatt gcaggatcat ggtacgctgg
    24121 cagagcatga tatctatatt gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt
    24181 tttgcagtga gcgtaatgcg cgggaagatc gcctgtttgg cgatgcgttt gcatttatct
    24241 gagatataaa aaaacccgcc cctgacaggc gggaagaacg gcaactaaaa ctttattcag
    24301 tggcatttag atctatgtcg catccggcaa tcggtgcacg atgcctgatg cgacgctggc
    24361 gcgtcttatc aggcctacat tggtgcagga tcggtagacc ggataaggcg ttcacgccgc
    24421 atccggcaag tggttaaaac cgctcaaaca ccgtcgcaat accctgaccc agaccgatac
    24481 acatcgtcgc cagaccaaac tgaacgtctt tgcgttccat cagattcagc agcgtggtgc
    24541 tgatacgcgc accggaacag cccagcggat gacccagcgc gatcgcacca ccgttgaggt
    24601 tgatcttctc gtcaatctgc tcaatcagtc ccagatcttt aatacatggc aggatctgcg
    24661 cggcaaaggc ttcgttcatc tcaaacacgc cgatatcgct ggtagaaagc cccgcttttt
    24721 tcagcgccag cttcgaggcc ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa
    24781 cgaccgccat cgaacgcaca cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt
    24841 cactcatcac cagcatggca gctgcgccat cggaaagtgc agaagatgtg cccgccgtta
    24901 ccgtaccgtt tactggatca aacgccggac gcagcgtggc gagggcttcc acggtggttt
    24961 ccgggcgaat cacttcgtcg taattaaact gcttcaggac gccgtcggca tcgtgaccac
    25021 cagttgggat gatttcattt ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt
    25081 gtgaccgcgc ggcaaaggca tcctgcattt cacggctgat accgtgcata cgcgccagca
    25141 tttctgctgt taagcccatc atgcccgccg ctttggcgac attgcggctc aggccggggt
    25201 gaaaatcgac gccgtgactc atcggcacat ggcccatatg ctccacgccg ccaaccagac
    25261 atgcctgcgc atcgccggtc ataatcatcc gtgctgcgtc atgcagtgcc tgcatggatg
    25321 aaccacacaa gcgattgacg gtaaccgccg ggacagagtg tggtacttct gccagcagcg
    25381 ccgcgttacg ggcgatgtta aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa
    25441 tatcgtcgag ggccgccgct tccagcgccg ggttacgcgc cagcaggcga cgcattaaat
    25501 gagcggagag atcttctgca cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg
    25561 gggtgcgaat tgcatcgaca atgacaacct gttccattgt gactccttaa gccgttttca
    25621 ggtcgccaac cggacgggct ggctcaaccg gaggatagta cggttcgtta tgacgcgctt
    25681 tattacgcag accttccggc acttcataca gcgggccgag gtgctgatat tgctgtgcca
    25741 tatcgaggta ttttgcgcta ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg
    25801 gagggaagcc caggccgtag accagcgcca tatccgcttc cgccggagtg gcgataatgc
    25861 cttcctccag acagcgcacc acttcgttga ccatcgggat catcatgcgg gcgataatct
    25921 cctcttcgct gaaatcgcgc ttcggctggc tcacttctgc cagcaggtct tcaacggcgg
    25981 cgtcttcttc tttcttcggc ttacctttgc tgtcttcttt ataacgccag aaaccgaggc
    26041 cgttcttctg accaaagcgg ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt
    26101 tctgcatcct ctgcgggaag cctgctgcca tgacagcctg ggcatgatgc gcggtgtcaa
    26161 tgcccacgac atccagcaga tatgccggac ccatcggcca gccaaactgt ttttccatca
    26221 ctttgtcgat cttgcggaaa tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat
    26281 acgggaacag cacgcggtta acaaagaagc cggggcagtc gttaaccaca atcggcgtct
    26341 tgcccatctt gctcgcccag gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc
    26401 cgcgaataat ttctaccaac ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga
    26461 agttttccgg gcgtttcagc gcgttggcca gttcgctgat aggaatggtt gaagtgttag
    26521 acgccagcac ggtatcctgg cgtacttttt gttcggtttc tgccagtacg gcttttttca
    26581 ctttcgggtt ttcaacaacc gcttctacca caatatccac gcggtcaaat ccggcgtagt
    26641 cgagcgttgg gtggattgtg gagatcacgc cagccagttt cagaccatcg atcttgccgc
    26701 gctcaagctg cttgttcagc agtttcgcgg cttcggtcat gccgagggtt aacgacttgt
    26761 cgttgatatc tttcatgaca accggcacgc ctttccacgc agactggtaa gcgatgccgc
    26821 cgcccataat gcctgcaccc agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga
    26881 gtttcttcgc tttgcctttt acatattgat cgttaaggaa aatgccgacc agtgcgcggg
    26941 cttcgttggt atgcgccagc gggacaaaac ttttgttttc caggtttaag gcttcttcac
    27001 gaccaaaacg ggccgcagct tcaatggttt ttactgcggt gatgggggcc ggataatgtt
    27061 tccccgctgt ttgtgcgacc atccctttag cgatggtgaa gctcatggtg gcttcaatct
    27121 tgctcagttt tagtggttcc agcttcggct gacgttttgc tttccagtcg aggtcgccgt
    27181 taatggcctg gcgtaaaacc gcctttgcgc cttcaaccag tttttctgct ttgactacgc
    27241 catccaccag accgattttc agcgcctgat ccgcgccgac atctttaccg gcggcaatga
    27301 tttccagcgc actgtcagcg cccagcatac gtggcatacg tacagaaccg ccaaagcccg
    27361 gcatgatgcc cagtttggtt tccggcagac cgatgcgcag atccggcgtc gccagacgat
    27421 aatcggtcgc cagcacgcat tcgcagccac cgcccagcgc atagccgttg acggcagcaa
    27481 tggtcggcac cggcagatct tccaggcgat taaacacgct attggcaaag tgcagccact
    27541 gacttaactg ttcttcagga acgaggaaca gggacaaaaa ttcggtgata tcagcaccga
    27601 cgataaaggc tgctttgttc gaacgcagca gcagcccttt tagatctgat tgctgttcca
    27661 gcacgccgat ggcctcgccg aggctggcga cggtcgcagt gtcgagttta ttaactgaac
    27721 ctggggcatc aaataccagt tcggcaatgc catcttccag ccagtcaagg tacagggtgt
    27781 cgcctttgta aagcatgtca gtctcctgaa tccgcaaggt gatctggtcg taccagatgc
    27841 atcgaagtgt gtattttgtg ttaaaaatat gcaaataaaa gattaaagaa atgccgatct
    27901 gatcacgctc ggcagaaatc acgctctgga tgaacgatgt gctaagatgc ggagacttaa
    27961 ggtcaaaaaa acagaagggt aaaaaatgga atcactggcc tcactctata aaaatcatat
    28021 agctacctta caggaacgga ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact
    28081 tattcactcc ggcgaactgt tcaacgtttt tctcgacgat catccctatc cgtttaaagt
    28141 gaacccgcaa ttcaaagcgt gggtgccggt aactcaggtg ccaaactgct ggctgctggt
    28201 ggatggcgtg aacaagccga aactgtggtt ctatctgccg gttgattact ggcacaacgt
    28261 cgaaccgctg ccgacctctt tctggactga agacgtggaa gtgatcgcgc tgccgaaagc
    28321 cgatggcatt ggtagcctgc tgcctgctgc gcgcgggaat atcggttata tcggtccggt
    28381 gccggaacgt gcgctgcaac tgggtattga ggccagcaat atcaacccga aaggggtgat
    28441 cgactacctg cattactacc gctccttcaa aaccgagtac gaactggcct gtatgcgtga
    28501 agcgcagaaa atggcggtca acggtcatcg cgcggcagaa gaagcgttcc gttctggcat
    28561 gagcgagttc gatatcaaca ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt
    28621 accttacagc aacattgtgg cgctcaacga acacgctgcg gtgttgcatt acaccaaact
    28681 ggaccaccag gcaccggaag agatgcgcag cttcctgctg gatgccgggg ccgaatataa
    28741 cggctatgcc gctgacctga cccgtacctg gtcggcaaaa agtgacaacg attacgcaca
    28801 gctggtgaaa gacgtaaatg atgaacaact ggcgctgatc gcgaccatga aagctggcgt
    28861 tagctatgtg gattaccaca tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca
    28921 tcaaatcatc accgatatga gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt
    28981 tatgccgcac ggtatcggcc atccgctggg cctgcaggtg catgacgtcg ccggttttat
    29041 gcaggatgat agcggtacgc acctcgcggc accggcaaaa tatccgtacc tgcgctgcac
    29101 ccgtattctc cagccgggca tggtgttaac catcgaaccg ggtatctact tcattgaatc
    29161 gctactggca ccgtggcgtg aagggcagtt cagcaagcac ttcaactggc agaaaattga
    29221 agcactgaaa ccgttcggcg gcattcgtat cgaagacaac gtggtgatcc acgaaaataa
    29281 cgtggaaaac atgacccggg atctgaaact ggcgtgatgg aaagctggtt aattcctgcg
    29341 gcaccggtca cggtcgttga agagatcaaa aagagccgct tcataacgat gttggcgcat
    29401 accgatggcg ttgaggcggc gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat
    29461 gcccgccacc attgcgtggc gtgggtggcg ggtgcgccgg atgattcaca acagctgggt
    29521 ttctctgacg acggggagcc ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg
    29581 ggcagcggcg ttggggaaat taccgctgtg gtggtgcgct actacggcgg catattgctt
    29641 ggcaccggtg ggttagtgaa agcgtatggc ggcggcgtga atcaggcgct gcgccagcta
    29701 acgacccaac gcaagacgcc attaaccgaa tatactttgc aatgtgaata tcatcagtta
    29761 accggcattg aagcgttgct ggggcagtgt gacggcaaaa ttatcaacag tgattatcag
    29821 gcattcgttc tgctgcgggt ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag
    29881 ctggcggatt ttagccgtgg ttcattgcaa ttgttagcga ttgaagaata atccccactt
    29941 cgttttgcag aactaaggaa gcggcagaga tgcattttcg cgccattacc cgaatcgttg
    30001 gactactggt catcttattt tcagggacca tgattatccc tgggctggta gcactcatct
    30061 accgggatgg agcgggccgc gcttttaccc agaccttttt tgtcgccctc gccattggct
    30121 ctatgctgtg gtggccgaac cgcaaagaga aaggcgaact taaatcccgt gaggggtttc
    30181 tgatagtggt gctgttctgg accgtgctgg gtagcgtcgg tgcgctccct tttatcttct
    30241 cggaaagccc gaacctcacg attaccgatg cgttttttga atctttctct ggcctgacca
    30301 ccaccggggc cactacgctg gtggggctgg attcgctccc tcacgccatc ctcttttatc
    30361 gccagatgct gcaatggttt ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc
    30421 ctatcctcgg cgtgggtggg atgcagctct atcgcgcaga aatgcccggc ccgctgaaag
    30481 ataacaaaat gcgcccgcga attgcggaaa cggcgaaaac cctgtggttg atttatgtct
    30541 tgctgaccgt cgcctgtgcg ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca
    30601 tcggccatag ctttgcgact atcgctattg gcggcttctc gacacatgat gccagtatcg
    30661 gttatttcga tagcccgact attaacacta tcattgctat cttcctgctg atctccggct
    30721 gtaactacgg tctgcacttt tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg
    30781 atccggaatt tcgcatgttt atcggcgtac agtttacgct ggtggttatt tgtaccctcg
    30841 tactgtggtt tcataatgtc tacagttcgg cgctgatgac aattaaccag gcgtttttcc
    30901 aggtggtgtc gatggcgaca accgccgggt ttacgactga cagcattgcc cgctggccgc
    30961 tctttttgcc ggtactgctt ttatgttcgg cttttatcgg cggttgtgcc gggtcaacgg
    31021 gcggtggcct gaaagtgatc cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga
    31081 aacgactggt gcatccgaac gccgtctata gcattaagct ggggaatcgc gcactgccgg
    31141 aacgtatcct cgaagccgtt tggggatttt tctccgccta tgcattggtg tttattgtca
    31201 gtatgctggc gattatcgcc acgggcgtgg atgacttttc tgcctttgcc tcagttgttg
    31261 cgacattgaa taacctgggg ccagggcttg gcgtggttgc tgataacttt accagtatga
    31321 acccggtggc taaatggatc ctgattgcca acatgctgtt tggtcgtctc gaggtcttta
    31381 cattgctggt gctctttacc ccgactttct ggcgtgaatg atggagtaat acgtgaaaac
    31441 attaattctt ttctcaacaa gggacggaca aacgcgcgag attgcctcct acctggcttc
    31501 ggaactgaaa gaactgggga tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc
    31561 acaatgggaa aattatgacc gtgtggtcat tggtgcttct attcgctatg gtcactacca
    31621 ctctgcgttc caggaatttg tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc
    31681 cttttattcc gtaaacctgg tggcgcgcaa accggagaag cgtactccac agaccaacag
    31741 ctacgcgcgg aagtttctga tgaactcgca atggcgtccc gatcgctgcg cggtcattgc
    31801 cggggcgctg cgttacccac gttatcgctg gtacgaccgt tttatgatca agctgattat
    31861 gaagatgtca ggcggtgaaa cggatacgcg caaagaagtt gtctataccg attgggagca
    31921 ggtggcgaat ttcgcccgag aaatcgccca tttaaccgac aaaccgacgc tgaaataagc
    31981 acaaagaata aaaaatgcgc ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga
    32041 ataactccct ataatgcgcc actactgaca cggaacaacg gcaaacacgc cgctgggtca
    32101 gcggggttct cctgagaatt ccgacagaga aagcaaaaat aaatgcttga ctctgtagcg
    32161 ggaaagcgta ttatgcacac cccacgccgc tgagaaaaag cgaagcgg
//
LOCUS       DAZGTR010000045        31793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGTR010000045 DAZGTR010000000
VERSION     DAZGTR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.045"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..185)
                     /locus_tag="ACHLCY_002265"
     CDS             complement(<1..185)
                     /locus_tag="ACHLCY_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369518.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVS"
     gene            648..932
                     /locus_tag="ACHLCY_002266"
     CDS             648..932
                     /locus_tag="ACHLCY_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369519.1"
                     /translation="MQMELISRKEFDSRVTSGELDNLQAIKVKEGFCLIGNQSGINRV
                     FMLRRTDLKPFVWKNEIGPSSYAQTRGCHNLAFFYKDELSVVDIQGLQHV"
     gene            925..1830
                     /gene="rdgC"
                     /locus_tag="ACHLCY_002267"
     CDS             925..1830
                     /gene="rdgC"
                     /locus_tag="ACHLCY_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP0369520.1"
                     /translation="MFKHWKNITIYKLSREADLTDLEDKKKMILFTPCGSQDMAKFGF
                     VSPFGDNSEVIAMHGNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTE
                     KDSLKDEVLHSLLPRAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLP
                     AVPLSVENPIELTLTDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEIS
                     THLEAGKLVTKLALDWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDAD
                     FFLLTSEISCLVDALVNALGGEAKQ"
     gene            1827..4868
                     /locus_tag="ACHLCY_002268"
     CDS             1827..4868
                     /locus_tag="ACHLCY_002268"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFP0369521.1"
                     /translation="MKELCYGSVCSGIEAASIAWEPLGMRPAWFAEIEPFPSAVLALR
                     WPHVANLGDMKKLAKKVLAGEIESPDVLVGGTPCFTAGHMVLCKNGYKPIENVCPGDY
                     VVSHLGRLQQVKRVGSKIANTGLLNAVGQPLGIRTTNDHPFLAVRWKAQNTRKNGTYF
                     KRELLSEPEWRAACDMPGYQWCALTNFNIAFPDICSRFLSEEQAMYLAGAYVGDGYIR
                     RWRGKSKKAVVFGINCQKLRKFHCRIPENIFSVASEIRGSIKVTLNDTCYANWLNEHF
                     GELSHAKRIPAWVMSHPLRHVFLQGYLDTDGTPSGKAGFRINSVSSALAWGVAGLSQT
                     CGYVSSVSFIEVEPKKVIEDRVVNQRNYYQVTICPQKLSRKSRLAHGMLLRTVKEFKS
                     VGLDTVYNIEVEGDHSYILNGAVVHNCQAFSIAGLRGGLDDERGALTLKYVELANAID
                     DKRAESFLKPAVIVWENVPGVLSSADNAFGCFLAGLAGEDVPFEPGDRPESGKSNAFW
                     RWDGKTGCHVPKWPQCGCIYGPQRKVAWRILDAQYFGVAQRRRRVFVVASARTDLDPA
                     TVLFEFEGVRRNIAPSRGEGKETTRYTSNIAIRTCDDTNIIAMAHGQGGAEIKTDNSA
                     PTLTCNHEAPIVLLGDGRMRRLTPVECERLQGFPDWHTLIPTEKRKKVNSDELAYLHN
                     HYPDLSEEEAAMLAADGPRYKAIGNSMAIPVMRWIGDRITKAVCRQKEGSETKERKVK
                     PAAEFERSIFKWAGGKFGVLEQIFRYLPEGKRLIEPFVGGGAVFMNAGYQENLLNDVN
                     ADLINFYKTLQREAHSLITLAHRFFQDYNTQEGYLAVRNAFNKQVYDDLHRAAAFLFL
                     NRHCFNGLTRYNQAGEFNVGYGKYKTPYFPLQEMEAFLGAEGRSEFVCGDFAAVIEAA
                     GEGDVIFCDPPYEPLPNTEGFTNYSGHDFKFEEQKRLVSLLTDAHRRGAKVLITNSGA
                     PNIRELYHDSGFRVEPLFARRSVSCKGDTRGVAHDVLGILL"
     gene            5541..5616
                     /locus_tag="ACHLCY_002269"
     tRNA            5541..5616
                     /locus_tag="ACHLCY_002269"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5574..5576,aa:Asn,seq:gtt)
     gene            5619..5694
                     /locus_tag="ACHLCY_002270"
     tRNA            5619..5694
                     /locus_tag="ACHLCY_002270"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5652..5654,aa:Thr,seq:tgt)
     gene            6785..7612
                     /locus_tag="ACHLCY_002271"
     CDS             6785..7612
                     /locus_tag="ACHLCY_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0369522.1"
                     /translation="MKKTLISAAIILGSLCLTGCDRVEPGNVGIKVNKLGDDKGIGEV
                     VGVGRYWTGLNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPAKVTTV
                     FQTYRKGVDDITDTDLRQKVADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQREQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPGVMDLEAINKWNGTLPQYMTSNTAVPFVPVK"
     gene            7640..7715
                     /locus_tag="ACHLCY_002272"
     tRNA            7640..7715
                     /locus_tag="ACHLCY_002272"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7673..7675,aa:Ile,seq:cat)
     gene            7996..9360
                     /gene="dnaB"
                     /locus_tag="ACHLCY_002273"
     CDS             7996..9360
                     /gene="dnaB"
                     /locus_tag="ACHLCY_002273"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0369523.1"
                     /translation="MSASPLESMPNSLSAEQAVLGGLMLDNCRWDEVADRIVADDFYT
                     SAHREIFSEMERLLSHGKPIDLITLAEALEQNGKLERAGGFAYLAEMSKNTPSAANIC
                     AYADIVRERAVVREMISVANEIAEAGYAQDGRGSNELLDMAERRVFEIAEKRQKSGSG
                     PKDIASILDATVSRIEELFQRPHDGVTGLDTGFTDLNKKTAGLQASDLIIVAARPSMG
                     KTTFAMNLVENAAVRNDKPVLVFSLEMPSHQLMMRSLASLARVDQTRIRTGQLNDEDW
                     ARVSGAMGILLDKQNIFIDDSSALTPTELRSRARRVYKENGGLSMIMIDYLQLMRVPE
                     LQDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGAIEQ
                     DADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQYSRFDNYAGADW
                     QEDY"
     gene            9360..10358
                     /locus_tag="ACHLCY_002274"
     CDS             9360..10358
                     /locus_tag="ACHLCY_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369524.1"
                     /translation="MQWNEEKPMNILIIGRKFEAISDVKTYTEMWAYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATACSAKAILAPGLRYFTTVPREIGMQLCRRFS
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQELFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLAVPYEARTLTDDGLITIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALILTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLDTFITQKPSRGNG"
     gene            complement(10406..11038)
                     /locus_tag="ACHLCY_002275"
     CDS             complement(10406..11038)
                     /locus_tag="ACHLCY_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFP0369525.1"
                     /translation="MGSLTGKYRAVVVSVDDPKGLMRTQIRVVGMMDGLPDASLPWAE
                     AILSNANTFSPFLPGDKVWVEFPYNGDSRWPLIIGYAQDASGGAPNVPPEASGQGEGY
                     VPPEVEGAPAQPSTSAKKDFISSRNGLMEVRTAGGAWAVTHLKSGTTIGFNEAGELYA
                     ISQGPAFISSAGNLDIKSGADVALKAGGSMAIEASGNLSIKAAQVSVVKA"
     gene            complement(11031..12047)
                     /locus_tag="ACHLCY_002276"
     CDS             complement(11031..12047)
                     /locus_tag="ACHLCY_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0369526.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEIPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTVLIYAFSNPVRLLKVPSTSAQYFVDKPPSAVVSTLAPGLKVNADSFRKTSTYHLNV
                     GEKPTKVLQEIARDTGSMCWASRGMINFKSMEKMANAAPSLTYESANPNTSGFTISQF
                     NILNADYEYQRRHNYRMASYDMTKGVVYSGNQEDPIKFTSNPDPTALANYNKFILPRL
                     DMLVEGNAALTPGTTLKIVVHNTAGDGELDESIPDKMIVMSVTHFEDRFRFVSRAQLG
                     VVNG"
     gene            complement(12049..12834)
                     /locus_tag="ACHLCY_002277"
     CDS             complement(12049..12834)
                     /locus_tag="ACHLCY_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HFP0369527.1"
                     /translation="MILNNQEWLLAIFKKKGLTPTGKLEFATIDGIDSALAQALNEAF
                     DSQVVSFNDRINQSFREFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTASAGPV
                     NKLAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIA
                     SDEKESLGMVTTALAFWLRMYASLGQASFTHTANVGGYEIPVTCYIEGQKSIAFQDLT
                     TGTADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            complement(12821..13549)
                     /locus_tag="ACHLCY_002278"
     CDS             complement(12821..13549)
                     /locus_tag="ACHLCY_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0369528.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRNKNAPETAPEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
     gene            complement(13553..14770)
                     /locus_tag="ACHLCY_002279"
     CDS             complement(13553..14770)
                     /locus_tag="ACHLCY_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0369529.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEDIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TANRKNAELKASRVREAQARAEADALKKALQTQANNLIKFFMSQGMDKAAATAQARSI
                     LETQINEARKRKG"
     gene            complement(14780..15040)
                     /locus_tag="ACHLCY_002280"
     CDS             complement(14780..15040)
                     /locus_tag="ACHLCY_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369530.1"
                     /translation="MCESNDKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAG
                     LEDESQGMTVGKKLKLPPATWIREMIRHFQYDGQVIGTLSIA"
     gene            15304..15549
                     /locus_tag="ACHLCY_002281"
     CDS             15304..15549
                     /locus_tag="ACHLCY_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369531.1"
                     /translation="MAERVDDAELSMNQLEALKDMAIDNIRKQAQVVSQVFTGKCRYC
                     NEPIESGIYCDAECAQWHREEQAAKQRKYGMRPAGFD"
     gene            15552..16130
                     /locus_tag="ACHLCY_002282"
     CDS             15552..16130
                     /locus_tag="ACHLCY_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFP0369532.1"
                     /translation="MLRFTEQEYLAFSKRRQKGWVKPKPKKDPFLSLAPVKEVSPHAK
                     ALAALAKNPDLRVGNCEHYEQVFIFDYFERNYPEIYELLHATPNGGKRSKATAGKMKA
                     EGQKKGYPDMSLDKACGIYHGMRIELKEPNGKAPTKEQIAWMRRLREEGYYVVLAYGA
                     EQAITAILEYISLKKGEAIEHVLNGDKWLHAA"
     gene            16197..16352
                     /locus_tag="ACHLCY_002283"
     CDS             16197..16352
                     /locus_tag="ACHLCY_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369533.1"
                     /translation="MSSKVNYESLASVMPRNKQETDAVVDPVIAEMNARLEAEFAAEN
                     EHTTQGD"
     gene            16854..17480
                     /locus_tag="ACHLCY_002284"
     CDS             16854..17480
                     /locus_tag="ACHLCY_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369534.1"
                     /translation="MFPKNKFRGSDRVIIVGSGPSAANFVAPRGVPIIAVNGAIDWLN
                     RASYFFTLDPSPDNMRRVGRGRRRRGVCYCMALPDVKEREVRDGVLCFRRVAERGTEP
                     KNTNSPEWWAWRWSAHFGLCEDENEIASGNSAYGALNLAFHIGFKHVALVGVDATQEP
                     RVHSGGTPKNLSHLPLLFQSAREQIDVVSCGKMGGIPQMTLKEWLKNT"
     gene            17477..18154
                     /locus_tag="ACHLCY_002285"
     CDS             17477..18154
                     /locus_tag="ACHLCY_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFP0369535.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPVQ
                     DLLISVGDLIDRGTENVECLELLQMPWFRAVMGNHERLMIDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILARALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQHYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            18151..18852
                     /locus_tag="ACHLCY_002286"
     CDS             18151..18852
                     /locus_tag="ACHLCY_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369536.1"
                     /translation="MKIALVLRSGGDYNASDVQWLVNQLPKGYEIICLTDLKRLHVPG
                     VKVVPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILEDPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFEQESE"
     gene            19154..20416
                     /locus_tag="ACHLCY_002287"
     CDS             19154..20416
                     /locus_tag="ACHLCY_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369537.1"
                     /translation="MSTSAQNQSIENVSIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLEIEH
                     LKRQQQDAIEDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLAEAELPFEPEFIDDLAAKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            20490..20996
                     /locus_tag="ACHLCY_002288"
     CDS             20490..20996
                     /locus_tag="ACHLCY_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HFP0369538.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     KDWNEFNLACDTDTPITPVIDMVRQLFNVYTVWFVTGRCEIARDKTRAWLRKYVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVARVFRRHGVLTLMVREY
                     ENALLHQQ"
     gene            21191..21931
                     /locus_tag="ACHLCY_002289"
     CDS             21191..21931
                     /locus_tag="ACHLCY_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369539.1"
                     /translation="MKGEVKERGMIFNDEMVRAILEGRKTQTRRIMKNQPAEVGPEAP
                     VMVRKIGAGFQWYGADGVSSVFNCPFGIVGDRIWVRETWAILGNEDGCSVDWNDNLCR
                     GDEKNAARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRI
                     LLEITNVRVERLNDISECDARDEGVQPAGSLLPDHPGTFLTPKGDFAMAKVAFQRLWE
                     SIYGEESWNANPWVWVIEFERIQGATSE"
     gene            21924..22184
                     /locus_tag="ACHLCY_002290"
     CDS             21924..22184
                     /locus_tag="ACHLCY_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369540.1"
                     /translation="MSESKCQINGNKIEPCAALAQSLEHDAEYTTRKGLLKYKIYNHE
                     LIHSQDLIMLRSGEFSKSPIRVSFCPFCGESLKTWEAEATSE"
     gene            22177..22821
                     /locus_tag="ACHLCY_002291"
     CDS             22177..22821
                     /locus_tag="ACHLCY_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369541.1"
                     /translation="MNNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEEAQ
                     KAWSCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIESDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINNFNRRTMVQGA
                     GLVMPDWLKKQNRRKKSRSGKVRWNCPHCGKITWQYSPYDFEGCSDYNCEGWRE"
     gene            22818..23471
                     /locus_tag="ACHLCY_002292"
     CDS             22818..23471
                     /locus_tag="ACHLCY_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0369542.1"
                     /translation="MTIDYQVLREAAEKATPDEWVAFISTDTGTYAVHTPGDERCEDV
                     IKWTGFDGQKNAENNARHVAAFNPKVALELLGEIKCLEDTNIDATCRISELETNLAAL
                     VAENAGLKHAMAVTLEHVSVTDAGQAGVAAMIINDALHHSETPATDAFLSEVRAQGVD
                     AAIEAAKNLVAQEYEYKDFKAAQSDCCMHPGSDLVGKVEMTEWLVDFAAQLRKGGNQ"
     gene            23468..24220
                     /locus_tag="ACHLCY_002293"
     CDS             23468..24220
                     /locus_tag="ACHLCY_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0369543.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDDLLSEQELKDYGVD
                     IDALNAFKFLTGPETVLALLDERERNQQYIKRRDQENEDIALTVGKLRVELEEVKQHA
                     EELSETKAVRNQWRPDICPITGRAFFMWIEHPTLGNVPTYGGPLDSYTIPTKDGDGEF
                     SCERYDHDFGGWVESECLGLYLIDDREQCRVYELEERVKELDAREISLPERSSMLHRT
                     DFHDDYQTVMAYKVSEVIAAIRAAGIRIKGGE"
     gene            24220..24396
                     /locus_tag="ACHLCY_002294"
     CDS             24220..24396
                     /locus_tag="ACHLCY_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369544.1"
                     /translation="MRVACIGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     AARRAAKRRRRAKR"
     gene            24393..24587
                     /locus_tag="ACHLCY_002295"
     CDS             24393..24587
                     /locus_tag="ACHLCY_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369545.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWKNHCYLRTKGE"
     gene            24589..24767
                     /locus_tag="ACHLCY_002296"
                     /pseudo
     CDS             24589..24767
                     /locus_tag="ACHLCY_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25101..25547
                     /locus_tag="ACHLCY_002297"
                     /pseudo
     CDS             25101..25547
                     /locus_tag="ACHLCY_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749537.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25567..25776
                     /locus_tag="ACHLCY_002298"
     CDS             25567..25776
                     /locus_tag="ACHLCY_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369546.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            25773..26441
                     /locus_tag="ACHLCY_002299"
     CDS             25773..26441
                     /locus_tag="ACHLCY_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0369547.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     ADPVKRVNSDQMHRVCLEANRYLDKYDAMAKEVNKLLGRIAPPAPVLPHEWRLSNAQA
                     FIERYSPPSVEEAALFAWNSCRAAMLHGAEPVSQTYKLNQLSGNSPATPDGWISCSER
                     MPNTKTAVLVAVEFDRKGDWRMKWATYIPGHPDANDGWIIPGASWKPSHWMPLPEPPQ
                     EVNQ"
     gene            26524..26757
                     /locus_tag="ACHLCY_002300"
     CDS             26524..26757
                     /locus_tag="ACHLCY_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369548.1"
                     /translation="MGKITFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIKKMELLTH"
     gene            26936..27229
                     /locus_tag="ACHLCY_002301"
     CDS             26936..27229
                     /locus_tag="ACHLCY_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369549.1"
                     /translation="MWRGNSHGKSQMILTEYKLDHKTNKSRSVYLLRHNSRVRNTVLE
                     QNLTVEMDNYGGFKPTISLDDFPRGLSEREAMLKLAEWLQRLSIAIEDNWSEP"
     gene            27236..27610
                     /locus_tag="ACHLCY_002302"
     CDS             27236..27610
                     /locus_tag="ACHLCY_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369550.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDHSFSLLFNS
                     LRILPETEVGELHLWILGFIAAVVGIAVWPFIFWLVAVVVWFKCKTYRRKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            27592..28272
                     /locus_tag="ACHLCY_002303"
     CDS             27592..28272
                     /locus_tag="ACHLCY_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369551.1"
                     /translation="MAQKEMTTLTTAYLQQLVFFAGEATCHPDANYLLEFERLASPGI
                     VLELAQQVLALRQKEQHESNTCRLNFEQWLEQQRGKIDVDCGCVSTETFMHWLRVAYE
                     AGNYPDIPDSSVPAPGKGVTGERIRIKPHVYRELVNRLHDTAIKCAGTQQLRERISRV
                     LGDVITPDHHKQAEKSDLERCHLEAALNIKPGHTLGIIDALLVHKMARALLPLVAEKH
                     EADHANES"
     gene            28545..28823
                     /locus_tag="ACHLCY_002304"
     CDS             28545..28823
                     /locus_tag="ACHLCY_002304"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369552.1"
                     /translation="MFRPRPIPPKITNGYQPNTPEIQGSEGLKPPPAPRIPPVARNVS
                     LRRELILALSPVLAARQDWVSNDDLAEKITSLVDKILQIGNEITLIHW"
     gene            28945..29334
                     /locus_tag="ACHLCY_002305"
     CDS             28945..29334
                     /locus_tag="ACHLCY_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0369553.1"
                     /translation="MGFPSPAADYVESRISLDQQLIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHIINDARGGEFDDSPAI"
     gene            29469..29891
                     /locus_tag="ACHLCY_002306"
     CDS             29469..29891
                     /locus_tag="ACHLCY_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369554.1"
                     /translation="MSSSAFVTVVTGVSVFVLGRLIIKGVESYISFKEQLGEISHLLL
                     SNQAKLSNPRSDLKQEIIHDLKDAAAQLRVKYELLPFYIKMLHIGFRLVPSETEIRKA
                     AQELNLIASIHDGKTGELPYEHIAKIGLLLRIPTTYSA"
     gene            30000..30221
                     /locus_tag="ACHLCY_002307"
     CDS             30000..30221
                     /locus_tag="ACHLCY_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP0369555.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            30221..30601
                     /locus_tag="ACHLCY_002308"
     CDS             30221..30601
                     /locus_tag="ACHLCY_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP0369556.1"
                     /translation="MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEISDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVAATLRRLYGSAE"
     gene            30606..30785
                     /locus_tag="ACHLCY_002309"
     CDS             30606..30785
                     /locus_tag="ACHLCY_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HFP0369557.1"
                     /translation="MARKYNKLSREALKMLLDGVSRREVKQYLVGKQIGARTAIAVLC
                     RQEMVVLKQRMPGSR"
     gene            30813..>31793
                     /locus_tag="ACHLCY_002310"
     CDS             30813..>31793
                     /locus_tag="ACHLCY_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0369558.1"
                     /translation="MKAVITPFVQKELGVATFKVDQEVRKLVEAGRKFIMEPVPRELI
                     EHMDDGLVVSEQTMATNEALQPFFNSDELFRRIGGIDALVAWLRRKEGQCQAADRSWC
                     DNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKETLHNNRVNWILDVARQEMGLSDGH
                     DLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAATQDSGPLKEADIRPYDDRATAYV
                     QMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQSLKLPEYMDFVASRPCCGCGAAGA
                     GAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERDRDNWEKTHGRLAMHQR"
BASE COUNT         8396 a         6961 c         8311 g         8125 t
ORIGIN      
        1 gaaacatagc tggtaatagt gtcgggatag acaattgtat ccagaattaa tccggtcaat
       61 tcttctgcaa gcagttttgc tgtgttagca ctgtttcgtg ccgatgttat ggcaccaggt
      121 gttttcatcc caccggcggc ggccaatttt ttatatgcgg ataactggta gtctttttcc
      181 agcatgatat ctcctaactt acctgaacca ggccgtctcc ggctgcaacg gtagagccgc
      241 atgaaacagg atcaccaacg catacgatcc ctttcccgtt gacggtaaac catgccctgg
      301 ttgatatagc ttgcccgccg tgcgtgctgt tcccatcggt atgctgtgca tattgcttac
      361 catcaactaa cacttcgact ccgttgactt taagtagtgg ttcgctctct acgggaggcc
      421 tggatgggaa tcctccgtgc cccgaacaaa tgctgtcttt tgttgcaatg cttgccacgc
      481 catcaccaat tatttgctct gattttcgtt attttaactt aggttatttg tggtctgtat
      541 ggcgtttact tatcgcaaaa ttgctctaat aaatattgtt ttttatgtcg tattttcggt
      601 accattcagc catcgccctt caatgggcat ttgtttggag tcgtcagatg cagatggagc
      661 taataagccg caaagagttc gatagccgtg taaccagcgg tgaactcgac aacttgcagg
      721 ctatcaaggt gaaagaaggc ttttgcctca ttgggaatca gagcggaata aatcgcgttt
      781 ttatgcttcg ccgtacggat ttgaagccat ttgtctggaa gaacgaaatt ggtcccagct
      841 catacgctca aacgaggggg tgccacaacc tggccttttt ctacaaagac gagctttctg
      901 tggttgatat tcaagggtta caacatgttt aagcactgga aaaacattac tatttataaa
      961 ctttctcgtg aggcggatct gaccgactta gaagataaaa agaaaatgat ccttttcacg
     1021 ccatgcggta gtcaggatat ggccaagttc ggttttgtat cgccatttgg tgataattct
     1081 gaagttatcg ctatgcatgg aaatggtttt atccttgttg aagcaaagcg cgaaacaaaa
     1141 attcttcccc cgccggttat ccagcgagct attcaagaaa aaattgaaaa acttgagcaa
     1201 gaacaagcgc gtaaactgaa gaaaacagag aaggactccc tgaaagacga agttctgcat
     1261 tctcttctgc cacgggcttt ttcaaagttt tctgttatcc aggcgatcta cgacggttca
     1321 actaaacgta tctatatcaa tgccagcgcg cggcaggcag aggatatgct cgcgcttatg
     1381 cgtaagtctc tgggttctct tcctgctgtt cccctgagtg ttgaaaatcc cattgaatta
     1441 acgctgaccg actgggtacg tgatggtagt gctccacagg gatttcaaat gggggatgcg
     1501 gcagaactta aggcagtgct tgaggatggc ggtattgccc gagtgaaaaa gcaggatttg
     1561 ggaagcgatg aaatttccac acacctggaa gctggcaagc tcgtcactaa gttggcactc
     1621 gactggcaga accgcattaa atttacactg gaccataact tcagccttac cagcgtcaaa
     1681 tttgcggatg aattgcttga gcagaactct gatattgata gtgaagatgt tgcgcagcga
     1741 ctggacgcag atttcttcct gttgaccagt gaaatttcgt gcctggttga tgctctggta
     1801 aatgcccttg gcggagaggc taagcagtga aagagctgtg ctatggatct gtttgcagtg
     1861 gaattgaagc cgcgagtatt gcctgggaac cgttgggtat gcgtccggcg tggtttgctg
     1921 aaatcgagcc ttttccatcg gccgttcttg cgctccgctg gccccatgtt gccaaccttg
     1981 gcgacatgaa aaaacttgcc aaaaaagtct tggctgggga aatcgaatcc cctgacgtgc
     2041 tcgtcggggg aacaccatgt tttaccgcgg ggcatatggt tctttgtaaa aatggctata
     2101 aaccaataga aaatgtttgc cctggtgatt acgtagtcag tcatctcggg cggttacaac
     2161 aagtaaaaag agttggttca aaaatagcta atacggggtt acttaatgcc gttgggcagc
     2221 ctttaggtat aagaacaacc aatgaccatc ccttcctggc tgttcggtgg aaagcccaaa
     2281 acacccggaa aaatggcaca tattttaaga gagagttgtt gtctgaaccg gaatggcgag
     2341 cagcatgtga tatgccggga tatcaatggt gcgctctaac taatttcaat attgcatttc
     2401 cagatatttg ttctcggttc ttgtctgaag aacaggctat gtatcttgcg ggcgcttatg
     2461 ttggcgatgg atatattagg agatggagag gtaaatctaa gaaggcggtt gtttttggca
     2521 taaattgcca gaaattgaga aagtttcatt gccgcatacc agaaaacata ttttccgtgg
     2581 caagcgaaat ccgagggagc atcaaagtaa ccttgaatga tacgtgttat gccaattggc
     2641 ttaatgaaca ttttggcgag ttaagccatg ctaagcgtat ccctgcatgg gtgatgtcgc
     2701 atccattgcg tcatgtgttt ttacaaggct atcttgatac tgatgggaca ccaagtggta
     2761 aagcgggatt tagaattaat agtgttagtt ctgcgcttgc ttggggcgtt gcggggttgt
     2821 cacagacttg tggttatgtt tcttcggtca gctttattga agttgagccc aaaaaagtga
     2881 tcgaggatcg cgtggtaaat caacggaatt attatcaggt aacaatctgc ccgcagaaat
     2941 tgtcacgtaa atcaagattg gctcatggaa tgcttttacg aacagtcaaa gagtttaaat
     3001 cggtaggcct agatactgta tacaacatag aagtcgaagg tgatcattcc tatatcctca
     3061 atggtgcggt ggtccataac tgtcaggcat tcagtatcgc gggattacgt ggtgggcttg
     3121 atgatgagcg tggcgcgcta actttaaagt atgtggagct tgcaaatgca attgacgaca
     3181 aacgggctga gtcatttctc aaaccagccg ttatcgtctg ggaaaatgtc ccaggagtct
     3241 tgtcatcggc agataacgcc ttcggatgtt tccttgccgg attggctgga gaagatgtgc
     3301 catttgaacc aggtgatcga cctgaatcag gaaaaagcaa cgcgttctgg cggtgggatg
     3361 gcaaaaccgg ttgccatgtt ccaaagtggc cgcagtgtgg ttgtatttat ggaccgcagc
     3421 gaaaagtggc ctggagaatc cttgatgccc aatacttcgg agtggcacaa cgacgccgac
     3481 gcgtgtttgt tgtcgcaagt gctcgaacag atctcgatcc cgcaacggta ctttttgagt
     3541 tcgaaggcgt gcgccggaat attgcgccga gcagaggcga ggggaaggaa actaccagat
     3601 atacttcaaa catcgctatc agaacttgcg atgatacaaa cataattgcc atggcacatg
     3661 ggcaaggagg ggctgagata aaaaccgata attcggcacc aactttgaca tgtaaccatg
     3721 aagcaccaat tgtattgctc ggcgacggta gaatgcgccg tcttacccct gtcgaatgtg
     3781 aaaggctgca aggttttcct gattggcata cgttgatccc tacggaaaag cgtaaaaaag
     3841 ttaattcaga tgaactggca tatctgcaca atcactatcc agatttaagc gaagaagagg
     3901 ccgcaatgct tgcagctgac ggaccgcgtt acaaagcgat cggcaatagt atggcaatac
     3961 cagtaatgcg ctggattggc gatcggatta ctaaggccgt atgtcggcag aaagaaggaa
     4021 gtgaaacaaa agagcgaaaa gttaaaccag cggcagaatt cgaacggtcc atattcaaat
     4081 gggctggtgg aaaatttggt gttctggaac aaatctttcg ctatttgcca gaagggaagc
     4141 gcctgattga acctttcgtt ggtggcggag ctgtcttcat gaatgccgga taccaggaaa
     4201 atctgctaaa tgatgtgaat gctgacctga ttaactttta caagactctg caacgcgagg
     4261 cgcattcact tatcactctg gcacatcgtt tcttccagga ctacaacaca caggaaggat
     4321 acctggcagt acggaatgcg tttaacaaac aagtctatga tgatttacat cgcgcagcgg
     4381 cgtttttgtt cctgaaccga cattgtttta acggattgac gcgttacaac caggccggtg
     4441 agttcaatgt cggttatggg aagtataaaa ctccgtattt cccattacag gagatggaag
     4501 ccttcctcgg tgcggaaggg cggtctgagt ttgtatgcgg tgattttgca gcggtgattg
     4561 aagctgccgg agaaggagat gtcatctttt gcgatccgcc gtatgaaccg cttccaaata
     4621 cagagggatt cacgaactat tccggtcatg actttaagtt tgaagagcaa aaacgcctgg
     4681 tgtctctgtt gacggatgct catcgtcgag gtgcaaaggt tctcattact aacagtggcg
     4741 cgccaaacat cagagagctt tatcatgaca gtggcttcag agtggaacct ctttttgcca
     4801 gacgttctgt gtcttgtaag ggggacactc gaggtgtagc tcatgacgtt ttgggtatat
     4861 tgctctaata aatttattag tgtaatatta cctcaatgaa tcgtgattta tagagcgatt
     4921 tagctgttag ccgcgacagg cgcggcggta agcatggctg ggcctagtcc tcccagacaa
     4981 accaccgagt tgccaggttg accatgcgcc taagtggcaa cgccgaagtg cgttacgagc
     5041 gtccagtttg cccatcttcg ggtgggcgtt tttttcaggg ttttcgtcat ggttagcgac
     5101 tttgcggcgg tttagaaact gaccattaaa gtaaatgcaa acgatgatct gatgatggta
     5161 gcggcctaag aagccagacg ccacggggta tgagtcgtcc cccgtcaaaa aatcgaccgc
     5221 agagtgtccc cgtctgtgta ttagggaacg gggaggcaca acaggtaagg gcgctggtgt
     5281 gattaaccag atgaacgaga aggggccatc tgttggtcag cgtcctttcc tgttgcgtct
     5341 tcttttcagc gtaacagcgg tgcttaacag cactttgggt acagttccac gaatttacgg
     5401 gtatatcccg tcatgctgaa ggcgctaatc acgctggaag ccagggttgt gcatcccctg
     5461 ttaccgaatt gcagccaggg cgcggtgcgc cgaaaagcat acggaggtgg aagccctcgc
     5521 cggagacgta cccggcaagt gatggtgtag ctcagcggtt agagcggttg actgttaatc
     5581 aacgggtcga tggttcaaat ccatccacca tcgccaatgc cggtttagct cagttggtag
     5641 agcgcctgcc ttgtaagcag gatgtcagcg gttcgagtcc gttaatcggc accaacataa
     5701 caggtaagag cattctccct tatggggcct ggcttaaatg catcgagtgc tcttaccgtt
     5761 gtgatgaagt gcagatctta gaagcaacca gaagataagc atctggcttc acaacataaa
     5821 ccgcaggaac gaccaataaa cggtagtccg tatggagaac accccgttga ggaagaggcc
     5881 tggccggaac cgtaaccggc gactggtaat gtgtgtaaat ggagcgaaat atgtgggcgt
     5941 ttaacgggat acgggttgta acagcgatta ttaacggcga acttaagtat tcacgtttcg
     6001 agacaaatga acgtgatggg aaatgtattg gtgtgcggtt agtaagagaa tacgcataac
     6061 aactacaacg ttgagaacac tggcgtaacg gggtcatatc ccaatctacg aataaatgtt
     6121 gcgttgccgc gtgacaacca gtgttctcaa cattgtggtg aatgcacagg ctgatgtgcc
     6181 gcaactacag tagtgcgcgc tttgcggggc ttgctacaac cctgtgtcgg agttcagcac
     6241 cgaccatcac agtttgattc tctggcatga gcataacgct gaaataagtc cagtctggtg
     6301 cggtctgatc acccgccgtt agctccacga aacggagcac gtaacaggta agagcattct
     6361 cctgtaacgg gttcatatcc caatctacag gtccaccaag aatgctcttt ccgttgcggt
     6421 gaatgcggct aagcgcacgc ggggaaatgg ttatatctgt ccattatttc tccttgtttc
     6481 cacgtccacg gtggataacc agccaaagga caccgggagg aacccggcac cgcagctttt
     6541 ttattcgtta aataatggag tgagaggatg cagaacaatc cgaacaaatg tcgaacgcta
     6601 tgggtgcggt tatatattta tgccgtcctc tgtttgattg tgtcactggt tctgtatgtt
     6661 tggcttttgc caaatatgat ctcatctaac agcacaatac ttgtattgtt gggagtcctt
     6721 ctcgcgctca tttacccggc tttcgcagta gtcttttttc gtgaaaaaac caggaaatta
     6781 attaatgaaa aaaacgttga ttagtgcagc gattattttg ggttctttat gtctgaccgg
     6841 atgcgatcgg gtagagccag gtaacgtagg gatcaaagta aataagctgg gggatgataa
     6901 aggtatcggt gaagtagttg gcgttggtcg ctactggaca ggcttgaata ctgaagttta
     6961 tatcttcccg acctttaagc aaatgaagac atacgatgag ccgttcagct tccagatgag
     7021 cgacggaaca accattggtt accacatcgg cgtagcctac aaggttgatc cagcaaaagt
     7081 cacaacggta tttcagacct atcgcaaagg tgtagatgat attactgata ccgatctacg
     7141 ccagaaggtt gcagatgctc tgaaccgatt agccagcaaa atgaccaccg acaaatttat
     7201 cgacggtggc aaatctgaat tgcttgatgc agcccttaaa gacattcagg aagaaatgac
     7261 gccaatcggc attcaggtga tgagcctctc ttatgtaggt aagccagaat acccgccaac
     7321 agttattgac agtattaacg ccaaagtcac ggcaaaccaa aaaaccctgc aacgcgagca
     7381 agaggtcaaa caacgtgaag cagaggccaa catgctgcgc gcggaagctg ccggacaggc
     7441 tgatgctatt cgcacaaaag cccaggccga agctgatgcc attcgtttac gtggtgaagc
     7501 tctgcgccag aaccccggtg ttatggactt ggaagcgatc aacaaatgga acggtacatt
     7561 accgcagtat atgaccagta ataccgctgt tccgtttgtt ccggtgaaat aaaagcgtaa
     7621 gcaaaattgg cagtaatccg gccctttagc tcagtggtta gagctggcga ctcataatcg
     7681 cacggtcacc ggttcaagtc cggtaggggc caccatattt ggttgtaaca cggcgtctgg
     7741 cacatgcgtc gttagcggtc tggtgacgtt aaaggggtaa cctttcccct agctcaggca
     7801 acaaaccagg tagccggaat gtgcaagccc cgttcatagc gtcggactgc ggattcacca
     7861 tcctggcgat tcggtgtgac agccgggaag agtccggcgc attaatcctg attttctggt
     7921 gatgactcat atcgttagga gtgatttgag tatgccgatt atatctgaca ttcagcacgc
     7981 ctgggtggag tgctaatgtc tgcatcccct cttgaatcca tgccaaattc ccttagtgca
     8041 gaacaagctg tacttggtgg cttaatgctt gataactgcc gctgggatga agttgcagat
     8101 cgtatagttg ctgatgattt ttataccagt gctcatcgtg aaattttcag tgagatggag
     8161 aggttattaa gtcatggcaa accgattgat ttgataacac ttgctgaagc acttgaacag
     8221 aacggtaaat tagaacgcgc cggtggtttt gcgtaccttg cggagatgtc aaagaacacg
     8281 cccagcgcgg caaatatttg tgcttatgcg gatatcgttc gtgaacgcgc ggttgttcgt
     8341 gaaatgattt ccgtcgcaaa tgaaatagct gaagctggat atgcgcagga tggcaggggc
     8401 agcaatgaat tgctggatat ggccgagcgc cgcgtttttg aaatagctga aaaacgacaa
     8461 aagagcggta gtggtccaaa agatatcgcc agcattctcg atgcaacggt atctcgcata
     8521 gaagagttgt ttcagcgacc gcatgatggt gtaacggggc ttgataccgg atttaccgat
     8581 ctcaataaga agacggcagg acttcaggcg tccgatctca ttattgtcgc cgcccgccca
     8641 tcgatgggga agactacgtt tgcgatgaat ctcgtcgaaa atgccgcagt ccgtaacgat
     8701 aagcccgtat tggtttttag ccttgagatg ccgagccacc aactgatgat gcgctcactg
     8761 gcttctcttg cacgcgttga tcagactcgt attcgaacag ggcaacttaa cgacgaggat
     8821 tgggcgcggg tttctggcgc aatggggatt ctgttggaca agcagaatat ttttattgat
     8881 gactcaagcg ccctaacacc tacagagctt cgttcccgcg ctcgtcgtgt ttataaagaa
     8941 aatggtggtt tgagcatgat tatgatcgac tacctgcaac ttatgcgcgt ccccgagctg
     9001 caagataacc gaacgctgga aattgccgag atttctcgat cacttaaagc gttggcgaag
     9061 gaattacaag taccggtggt ggcattgtca caacttaatc gctcgcttga acagcgcgcg
     9121 gacaaacgac cggtaaattc agatttacgt gaatcaggag caattgagca ggacgcagac
     9181 ctgatcatgt ttctgtatcg cgacgaagtt tatcacccgg atagcgaaat gaagggcatt
     9241 gccgaggtaa ttatcggaaa gcaacgaaat ggcccaattg gcacggtgag attggctttt
     9301 aacggccaat actcacggtt tgataactat gccggtgctg actggcaaga ggattattaa
     9361 tgcaatggaa tgaggaaaag ccgatgaaca tcctgatcat tgggcgaaaa tttgaagcca
     9421 tcagtgatgt gaaaacatat acggaaatgt gggcttataa cctggcatgc gcctttagtg
     9481 aggcaggggt aacattgcaa taccatcgtc catattcccc tggcgtcgaa agccccgagg
     9541 attatgttga agctgtgttg actgctgcga ccgcatgttc tgcgaaggcc attttggcac
     9601 caggattgag gtattttact acggtaccca gggaaatagg catgcaactg tgtcgccgat
     9661 tctctggatg ggtagcccag gtatatgacg gttctatgct ggattcagca ccagtcgata
     9721 ttacttttac tgtccgcgat gatacctggc ggtacctgga taatcccggt cggttagagc
     9781 gtcataatcg ctttaacaaa catgttggat gggcagcgaa tcaggaactt ttccatctgg
     9841 aaaccaaaac agacgatgtt ctgcgtattt ttgtagacca cgctgcattt gatgttagtg
     9901 ggtttgatca ctccttaagt atccttatga accttcagcg cctggccgtt ccgtatgagg
     9961 ccagaacgtt gaccgatgac ggattgatta ccattgatcc ggggaatata tcggtaaccc
    10021 catacagacg gactccggta ccagcaaccg aatttgcagc tgaattgcgt aagagtgacg
    10081 tttttattgt tacgcatccc gaaagccttg gattaactgt tcttgaggcg gcaatgtgtg
    10141 gggcgttgat attaacgccg cccgattgtc ttccgccgga tcgcctggct ttggtgaacc
    10201 atatggttat caagtcgcgg attgattggg atgaggttat tgctcgcgtt gatcgcgtga
    10261 aaaatgctga aaaggtccag tgtcacacct ggtcggcaat cgcggaaaag atgcttgata
    10321 cgtttatcac ccagaaaccg tcgcgaggta acggataaaa acattgtacc cgtggtaaca
    10381 gaaaagcccg atcgccgggc ttttcttaag ccttgacaac agagacttga gcggctttta
    10441 tggatagatt cccgctggcc tctatcgcca tacttccccc cgccttcagg gcgacatccg
    10501 cgcctgactt tatatcgaga tttcctgcgg aagagatgaa tgccggacct tgagaaatgg
    10561 catataactc cccagcctcg ttgaacccga ttgttgttcc acttttcaag tgcgtaacgg
    10621 cccaggctcc gcccgccgtc cggacctcca ttagtccgtt ccgcgacgaa ataaagtctt
    10681 ttttggcgct ggttgatggt tgtgctggtg caccttcgac ttcaggcggt acatagcctt
    10741 caccttgtcc tgacgcttca ggcggcacat tgggagcgcc accggatgca tcctgtgcat
    10801 aaccgattat caatggccat cgcgaatctc cattgtaggg aaattctacc catactttat
    10861 cgccgggcag aaatggtgaa aacgtgtttg cattggacaa tatagcttct gcccacggca
    10921 atgaggcatc tggtaaccca tccatcatgc cgacaacgcg tatttgtgta cgcatcagac
    10981 ctttagggtc atcgacgctt accactacag cccgatactt ccctgtcaaa ctacccattc
    11041 accactccta actgtgctcg gctgacaaaa cggaagcggt cttcgaaatg agtcacggac
    11101 atcactatca ttttgtcagg gatagattca tcgagttctc cgtcacctgc cgtgttatgc
    11161 acgacaattt tcagcgtcgt acccggagtt agcgcggcat ttccttccac cagcatatcg
    11221 aggcggggga gaataaattt gttgtagttc gccagcgcgg taggatcggg attgctcgta
    11281 aatttaatgg ggtcttcctg gttacctgag taaaccacac ctttggtcat gtcataactg
    11341 gccattctgt aattgtggcg gcgctggtat tcataatcgg cattcaggat gttgaactga
    11401 ctaattgtaa atccggatgt gttgggattg gcggactcat aagtaagcga tggagcggcg
    11461 tttgccattt tttccatact tttaaaattg atcatccccc tggatgccca gcacatagaa
    11521 ccggtatccc gggctatctc ctgcaatacc ttggtcggtt tttctccaac atttaggtgg
    11581 tatgtggatg tttttctgaa tgaatcagca tttaccttca gaccaggggc aagagtggaa
    11641 actacggctg atgggggctt atcaacaaaa tactgtgcgc tggtggacgg aactttcaat
    11701 aaccgcaccg ggttactaaa cgcgtaaatc agtacggtat cgtccttgcg cggcgcttta
    11761 agaacgaaga actcttccga gaacaggatg ccgccatgac cttccggatc accaagtgaa
    11821 acagtcagta ttgttccaaa tttcaccccc agcttattga ccacgtaagc cgttgaatcc
    11881 ctgaccatga gcattagctg gggaccagat agctccccgg gttcgacata ggtacatcct
    11941 acgatcattt cgcgagggat ttcgttctgc ccaattgaaa cagattgcag gaatagctga
    12001 gtgcgttttg aatcagtttc cggggctgtg gtggtctttg tggccatctc attcctccag
    12061 aattttcgct tttaccgtta tggtgccggt ggtttgctgc atataagcca ggataggaag
    12121 ctccgccact actgtgaggt tcaatccaac cgcgaacagc ctgttgtcgg cggtgccggt
    12181 ggtcagatcc tgaaatgcga ttgatttttg cccttctatg taacaggtaa ccggtatctc
    12241 ataaccgccg acattggcag tgtgagtgaa agatgcctgc ccgaggctgg catacattcg
    12301 tagccagaat gctaatgcag ttgtaaccat cccaagagat tccttctcgt cactggctat
    12361 ccatagcgaa tattccagtg agaaagggat agtcgatacc agggcttcaa tctcatcatt
    12421 ttcattggtg acatgccctt catcgtaatt atcccggcac agttcaccct cataaattga
    12481 aaatgcggga gaacgagaca gattcacaag cggcattgcc agcttattta ccgggccagc
    12541 agaggctgta tctttgcgcc cggcgcgatc ggcttcaaat gacgacaacc actccttcac
    12601 atcactaaaa gtgccgagcg ttatgcgatc tcttggtgtg cgtttcagga actcccggaa
    12661 cgactggtta atgcgatcat taaagctgac aacttgtgag tcgaacgctt cgtttaaagc
    12721 ctgtgcgagc gccgaatcaa tgccatcaat agtggcaaat tccagcttac cagttggagt
    12781 aagacctttt ttcttaaaga tggccagtag ccattcctga ttattcagaa tcaccgatga
    12841 aattcccttc aaaggcgcgt gaaggcacgc aataaaacaa actgcctacc ctggcagtgc
    12901 cgtaattgaa tattttatgg atgtaccaga agcggcgaat ggttgtgccg tctgacagct
    12961 gttccagcca ttcgagcata gaacccactg gcacattgac ggcagctaac cgaaggatta
    13021 aagcactgtc gctaattccc gtattatcac tgccgtcgta tagcgcgtag aaggcgtcca
    13081 tctcatccgg gcagtcgagg gccgttatca gttctggatc ctgatagtca tatatgcgtt
    13141 ggttcggttc tattatttca ggtgccgttt caggtgcatt tttgtttctg taaggtattg
    13201 cgcgatacag aactgcatcg aatgagtcag ggtctagctt gattgctttg agccagtcca
    13261 tccgcacaag gttattaaaa actgcatgac cttgataacg gtggcgcaca ccagaatcac
    13321 taagcaggcc gtgatccaga ttgggaaggt gattgtcctc cacaggatca acaatattac
    13381 caacgttaac accatcggtt tcgatttcag catcaatatc ttcctcttca atcagttcag
    13441 aaccttcgcc tggaatatcc ggatccgatt cggtgtccgg gaggttatca ccagtcactt
    13501 gttgtgatgg ttctgtgtcc tcaaacatgt catcaaagaa accagccatc gattatcctt
    13561 tccgtttacg ggcttcatta atttgtgtct caagaatgct tcgcgcctgc gcggtggcag
    13621 cggccttgtc cattccctga ctcatgaaaa actttatgag gttgttcgcc tgcgtttgca
    13681 gggctttttt gagagcgtcg gcttcagcgc gagcctgggc ttccctcacc cgcgatgctt
    13741 ttagttcggc attcttcctg tttgccgtgg tgcgagcttt ttttaacaac cggcgaacgt
    13801 tgtccgtggc gctatctttg gcgcgtagtt ttttgcctaa tgcatcctga gatttcagat
    13861 ataactcata ctcacgcgcc gctttagcct gatccgtcgt tgttgtccgg ttgcgcgcga
    13921 gcgatttagc cagttcgcct ttgaaatagg ttgttgtctt ccgcttgtca tcgccgaagg
    13981 ccacctgttc agctgctttt tccagggcaa taatgatggc cttgtgccat gtgggagact
    14041 gaaaacgcgt catagcgtgc aaaacatgtt tgcaagccac accagtcaga tcagggttgc
    14101 ggattttggg gaatgcatac tcttttggcg gtgcgacagc atagttacca gccgtggcca
    14161 tataacggta ccagtattga tggcgtccac aatcacagtc gaaagatacc cggcccttgc
    14221 agagatcggc agcgattcgg gcttttttcg caccgtcttc agcaatatcc tcaacggctt
    14281 tatcccattc ctcaaatcga attctgacac ggtgatgctg gtggaccgac tcatccgagg
    14341 cattaacaga tatcaatgca aggttgtgtt ttagcccgag gaatgtcgcg gctttgatcc
    14401 ctgtgccatc agaaactttg ttgttagcgc gttttatatc aatgctggtg gactgcgcca
    14461 ccaactgagc ataggtaatg ccgggtaccg tgctcttgaa ttttgtttta tgagcctgcc
    14521 ttgaggtgtt gaagctgcgt atatcttcgg gcgtaaagta ggtgccatct ttctttttcc
    14581 caaggctgag gaatgcctca agttcgcggt tacgcatccc cataatcctt ggggtgagtg
    14641 tacgtcgcgc gtttcgccga ttctgacgct gctgtttacg gataagatcg aagaccttgt
    14701 taaagtcttt tgcacttaat ccatcagtct gatagcgacc aaggttgtcg cgagcatatt
    14761 cagttggcat ttatttccct tacgcaatgg ataatgtccc tattacctgg ccgtcgtatt
    14821 ggaaatggcg aatcatttcg cggatccagg tggcaggtgg gagttttaac tttttgccaa
    14881 cagtcatacc ctgagactca tcctcaagcc cggcggcgag cgtcacaacc cagcgtagct
    14941 ctgctatgcc ccacatacgg taagccagca aatccgggcg atattgctca tcgggaagaa
    15001 cgtaataaat cgtcagattc ttgtcgttcg attcacacat aagcatcacc tctttgcgta
    15061 gctctgccct gagtattgga tcggctatgt tgcggtcgtc ataccgcgac agaggatatt
    15121 gccgggtgct ttgggttgta gtgattgatg tagccatagt cagcctgcca gaaatagatg
    15181 atggtgattc taccgctagt catttgttga tcatttaact caataaaaga aaattattag
    15241 tgcaattttg gttgtgaaat gtatcattct gcctttaagt aggttcttca tgaggaaaca
    15301 aaattggcag aacgtgttga tgatgcagag ctgagcatga atcagttaga agctctcaaa
    15361 gacatggcca tcgataacat cagaaagcag gcacaggtcg tgagtcaggt atttacaggg
    15421 aagtgtcgtt actgcaatga accgattgaa tcaggcatct attgtgacgc tgagtgtgcg
    15481 caatggcaca gggaagagca ggccgcaaaa cagcgtaaat atggcatgcg accggcagga
    15541 tttgactgat tgtgttgcgc tttactgagc aagagtatct ggctttcagt aagcgcagac
    15601 agaaaggatg ggttaagcca aagcccaaaa aggatccgtt cttatcactt gccccggtaa
    15661 aagaagtttc gccacatgcg aaggcgcttg cagcactggc aaaaaatcct gatctgcgag
    15721 taggaaattg cgagcactac gaacaggttt tcatttttga ttacttcgag cgcaattacc
    15781 ctgaaattta tgagctgttg catgcaacgc ctaacggagg gaagcgttca aaagcaaccg
    15841 ccgggaaaat gaaggctgaa gggcagaaaa aaggttatcc ggacatgagt ctcgataaag
    15901 catgcggtat ttatcacggc atgcgaattg agcttaaaga accaaatggt aaagccccga
    15961 cgaaagagca gatcgcctgg atgcgcaggc ttagagagga aggctactac gttgttcttg
    16021 cgtatggtgc agaacaagcg attaccgcca tcctggaata cataagcctt aaaaagggtg
    16081 aggctattga gcatgtattg aacggcgata agtggttgca tgctgcttaa aataataaat
    16141 taattagtgc atatacgctc tttgtggtag tgcactttaa catcgggaga ataatcgtgt
    16201 catccaaggt taattatgag tcgctggcat cggtcatgcc gcgtaataaa caggaaacag
    16261 atgctgtagt ggaccctgta atcgctgaaa tgaatgctcg cctggaggct gaatttgcag
    16321 ctgagaatga acataccacc cagggcgact aggactgttt tttgtgtcgg tagcggtccg
    16381 tcactcactc gtgaggactg tgctgctata gaaaaaactg gctgttcaat catcgcggtt
    16441 aacaattcct ggcagatgtt cgatgacatt tatgccttat acgccggtga tttgtcatgg
    16501 tggaagcaat acggttccac cataccggga gggaggttcc gcaaagtgac agccaacctg
    16561 gcggcggcga aatcattttc gttggagtac aggcgatatt gtggaccggc ggaaggggta
    16621 aatagcggcg cgcaggctat cagtctggcg gctgaatcag gggctgaagt agtggtatta
    16681 gtcggctatg actgttctct gcaaaacggc cttcattggc atggtgcgca ccctcaagct
    16741 ctacggaatc caacgcaggt gtctatttca aaatggcaac agcagttcct ggatacccgc
    16801 aaaaaacacg cagatttaca tattttgaat gcaagtagga gcagtgcaat tcaatgtttc
    16861 ccaagaataa atttagaggc agtgatcgcg ttattatcgt cggcagtggc ccaagcgccg
    16921 caaactttgt tgcgccgcgc ggagtgccga ttatagcggt caatggggcc atcgactggc
    16981 ttaaccgcgc ttcttatttt ttcacccttg atccatcgcc agacaatatg cggcgcgttg
    17041 gtcgtggccg ccgtcgccgt ggtgtttgtt attgcatggc gctacccgat gttaaagaac
    17101 gtgaggtcag agacggcgtt ttgtgcttcc gtcgtgtggc tgagcgcggc acggagccaa
    17161 aaaatacgaa ttctcccgag tggtgggcgt ggcgctggtc cgcacatttc ggcctttgcg
    17221 aagatgagaa tgaaattgcc agcggcaata gtgcatatgg tgctctgaac ctggctttcc
    17281 atatcggatt caaacatgta gccctggtgg gcgttgacgc tacgcaagaa ccacgcgttc
    17341 attccggcgg cacgccaaaa aatctaagtc acctgccttt gttattccag tctgcgcgtg
    17401 aacagattga cgttgtttca tgcgggaaaa tgggaggtat tccgcagatg actcttaaag
    17461 aatggctgaa gaatacatga tggcaccaac aatttatcac cgtatcgacg gtaccaaata
    17521 caggaatgtc tgggttgttg gtgatctgca tggttgctac accagactga tgtccgaact
    17581 ccatcgtgtg gattttgacc cggtgcagga tttactgata tcggtcggcg accttatcga
    17641 tcgcggtact gaaaatgtcg aatgtctgga actattgcag atgccctggt tcagggcagt
    17701 gatggggaac catgagcggc tgatgattga tgcgttaagt cctgatggca acgtgaataa
    17761 ctggctaatg aatggcggac aatggttctt catgctggac actgatcagg aaatattagc
    17821 cagggcgctg gtggagctgg taaagcgtct gccctatatc attgagttga acaccgggca
    17881 agaaactatc gttatagccc atgccgacta tccggataat gaatatcaat tcggtaagga
    17941 ggtgccgctt ttcaacgttg tctgggcgcg agagcgtatc agtgattcga tggatgatat
    18001 tggtggcgaa atttcgggcg cagatcgttt tatctttggt cacactccgg tgaaaagccc
    18061 gaagacattc tggaatcagc attatatcga cactggtgcc gtattttgcg gaaacctgac
    18121 attgatgaaa gtgaaaggtg atggtgcagc atgaagattg ctttagttct tcgctctggt
    18181 ggtgactata acgcttccga tgtgcagtgg ctggttaatc aactgccaaa aggctatgaa
    18241 attatttgcc tgacagacct gaagcgttta catgtacctg gcgtcaaagt tgtcccattg
    18301 atcaaccagt ggcaaaagtg ccgtggctgg tgggcgaaaa tcgagttgtt ccgaccggat
    18361 ataaccgatg atctgttcta tctggatttg gacacggtta ttgccggtga tatacgccca
    18421 atccttgagg atccaccaac cagcttcacc atgcttaggg atttttacca tccacaatat
    18481 cgtggcagcg gtgccctgtg gataccaaat agtgtaaaag cgcatatctg gagttcattc
    18541 tggcaagatc cggaaggttg gatttctcgt tgtgtcacta ctgagtgctg gggtgatcag
    18601 gggttcttac ggaaggttat gggcgatgat acaccagcat ttcaggatct gtatccagga
    18661 tggtttgtaa gttacaaggc cgatgttgtg gaaccaggtt caaaatatgc gagcgcgcgt
    18721 tactccaggg ggaatggggc attaccaaag gactgccgaa taattttttt ccacggcaaa
    18781 ccgcgacctc gcgaagtgtc agaggattgg cttcccctca ttagctcgtt ttttgagcag
    18841 gaatcagaat aatattgctc taataattcc atatttttaa aacgtgatgt acactcatca
    18901 cgttttttat tagagcaatc tataaggtgc actatgtggc cattccgacg gaaatatcac
    18961 tactggctga tcgcctttgt tacgccgacc ggcggtatca ggcatgtcat caccaggtat
    19021 cgcaacaaga gactcacctt agccagaatt ttacaggctg ccataggtga gggactggat
    19081 acaaattgcg tagtccttcc tccttcatac ttaggaaaaa tgaccgaagc acaagctaat
    19141 acggaacttt gaaatgagca cttcagcaca aaaccaatca atcgaaaatg tatctatccc
    19201 tgatgtcctg aatgccggta tcccggccat tatccagaac atccgggccg cgcaacgccg
    19261 cgttagttgt gatgacctca cagcacgttt ttttgataat gcggttcagt cagcggagat
    19321 gcttcacgca cagcttattg atgtttataa cgcagaagct gatagccata actccctggt
    19381 agatgcagct gaaaatatgc agttggatct cggtctgaag ggtaaagaaa ttgaagagct
    19441 tcagctggaa attgaacatt tgaaacgcca gcaacaggac gcgatcgaag atgcgacgca
    19501 tgacgccaac cagcgtgctg ataatgccga acgtataagc attgagctgg aaacaaaact
    19561 caatgaaatg accgcgatgg ttgaactgcg gaactcacag atttcaacgc taaaatctca
    19621 atataaagag atcatgaaac ttgatccttt taaccttgag aaacgctata acaaagctaa
    19681 aagcgagcga caggaactgc gtaagcaggt cgccgacctt aaccaacagc tcaaaaaaac
    19741 tattaaagat gcaagcgaag cgcgcgtggc atttgctaat aaaaaagcag aggttaccgc
    19801 gctggttaat gagaatgcca aatttgcgac gctcaagaag gaaatgtatg gcattactga
    19861 gcgccgtttc cctgcaagca aacttcatcc gacgttaggg caaatctcct tcttcccgcg
    19921 cctcctggct tatgggatct catcgcctaa agagttcaat aacgagcgtc cttatatcgt
    19981 ttctaagctg gactttgctt atcagttctg ctgcgacatg ggctatgcca ttgatatccg
    20041 gatcaacgaa tggttgatgc caaacttcca gccgttggcc attttccgcg agttccagcc
    20101 ggaaggttgg gtagagttct tccatgaatt gatctgtaaa gagatggaaa gccgccgccc
    20161 ggaactggtc cgtcgagttg agtgggcgca agaggttatg ttggcagagg cagagctgcc
    20221 gttcgaaccg gaatttattg atgatctggc agctaaaggg ctgcataccc tgtttgatgt
    20281 ggttacccgc cgtcatgagc agttggttgt cgaattgggt ttagaggaaa cagcggcaag
    20341 aagacttctc gatgtttgct atgcacgtag cgatgcatgg gaaaaagaga acggcggcac
    20401 tatttacgtt cgctgatagt tacagtgtca cttttaatgc tggtggagtg cgtcccacca
    20461 gcattttttt cgtccaatga ggagggcatt tgagtatttt caataaacac gcacaccagg
    20521 aacgtccgta tatcgtcata gtagatattg atgggacgat atcggaggca acggaagaca
    20581 gactgcattt gcttccgcca ccaggtaaag gtgcattaac aaaggactgg aacgagttta
    20641 atctcgcctg tgacaccgat actcccatca ctccagttat tgatatggtg cgccagttat
    20701 ttaacgttta cacggtctgg tttgtaaccg ggcgctgtga gatcgcaagg gataaaacac
    20761 gagcttggct gcggaagtac gtaacaaacg gggctgagcc tttgctatct atgcgtcctg
    20821 ccaccgatga cagaaatgac ggcccagcaa agattgatct ccttaagaaa attggtctga
    20881 gtaaaattgc gttcgcgctg gaagataaga ttgaagtggc gcgtgttttc aggagacacg
    20941 gcgtacttac gttaatggtc agggagtatg aaaacgcgct tctccatcag caataattgc
    21001 tctaataaat cttgattttt aaaacagaga aagtgaaaat aaaaacatgc cgcaagacgc
    21061 ggcatgtatc caatcaatca caggagctga aaatatgaac acggcattca aaatcattat
    21121 ggccgcgatc tatttctggc tgttctctat cacttttggc ggcatcgtcg cacatgggta
    21181 agggggatgc atgaaaggcg aagtgaaaga gcgcggcatg attttcaacg atgagatggt
    21241 ccgggccatc ctcgaaggaa ggaaaacgca gacgcggcgg ataatgaaaa atcagcctgc
    21301 ggaagttggt ccagaagcac cagtgatggt tagaaaaatt ggtgcaggtt ttcagtggta
    21361 cggggctgat ggtgtaagca gtgttttcaa ttgccccttt ggtatcgtcg gcgatcgaat
    21421 ttgggttcgt gaaacatggg cgatattagg caatgaggat ggttgcagtg tggactggaa
    21481 cgacaacctt tgtcgtggcg atgagaagaa cgcagcaagg atttatcggg ccagttgcga
    21541 gcaaaaacct ggtgattacg gcttatggtc gatacccgat gatgccgact ggaaacctca
    21601 cactgtgaat gaaaagtttg atggtgggtg gcgtccatca attcacatgc cgcgatgggc
    21661 atcacgcatt ctgttggaaa ttaccaacgt gcgcgttgag cggttgaacg atatcagcga
    21721 atgcgatgca agggatgagg gcgttcagcc tgctggaagt ttgcttcctg atcacccggg
    21781 aacattccta actcccaagg gagatttcgc aatggccaag gttgcgttcc agcgcctgtg
    21841 ggaatccatc tatggtgaag aaagctggaa cgctaatccc tgggtgtggg taatcgagtt
    21901 tgagcgtatt cagggggcaa ccagtgagtg agtcaaaatg ccaaattaat ggcaacaaga
    21961 tagaaccgtg cgcggcgttg gcacaatccc tggagcatga cgctgaatac acgacgcgaa
    22021 aaggtctgct gaaatacaaa atctataacc atgaattaat tcattcacaa gacctgatca
    22081 tgctgcggtc tggtgaattt tctaaatcgc ctattcgagt ttcattttgc ccgttctgtg
    22141 gtgaaagtct gaaaacgtgg gaagcggagg caaccagtga ataaccgctt ttacatgatg
    22201 tgcttgcgtg aaactgtggg taataacgcc tcattccatt gccataacgg caatggttac
    22261 agttctgata tcgatcgcgc tcatgtttac acgctggaag aagcccaaaa agcctggagt
    22321 tgtgggcgag atatcgatca gcctgtttgt gccgatagtg tggatgcaat ggctgtgtgg
    22381 cacgttgatt gccagtacat ccctacagaa agcctgattg agtcagattg cactgcgtat
    22441 gtggcctaca aaaaaggtag ctggaacggc aacgatgttt actggcttca acacggtgga
    22501 ttgccaacag atgacttcag taaagcgacc atctttagcg tcgccaacaa aaacgaacca
    22561 ggaatagttt ggttgccatt ttccattgct gatgcagcaa agcgccggac gttcaatatc
    22621 aataacttta accgcagaac aatggttcag ggcgcaggtt tggtcatgcc tgactggttg
    22681 aaaaagcaaa acagaagaaa gaagtcgcga agcgggaagg tgcgttggaa ttgtccgcat
    22741 tgcggaaaaa taacctggca gtacagccca tatgattttg aaggctgtag tgattacaac
    22801 tgtgaaggat ggcgagaatg acaattgact atcaggtact gcgtgaggcg gcagaaaagg
    22861 caacaccaga cgaatgggtc gcatttattt cgacggatac tggtacttat gcggtgcaca
    22921 cgcccggtga tgaacgatgt gaagacgtta tcaaatggac cggctttgat ggacagaaaa
    22981 atgcagagaa caacgctcgt catgttgccg cgttcaaccc aaaggttgca ctggagctgc
    23041 ttggtgaaat taagtgcttg gaggacacaa atattgatgc cacgtgccga atttcagagc
    23101 ttgagactaa tctcgctgcg ctggtggcag agaacgctgg gctgaaacac gcaatggccg
    23161 taactcttga gcatgtgtcg gtcacggatg cagggcaggc cggagttgct gcaatgatta
    23221 tcaacgatgc cctgcaccac agcgaaactc cagctaccga tgctttcctg tctgaagtgc
    23281 gggcgcaggg ggtggatgct gctatagaag ctgcaaaaaa tctggtggcc caagaatatg
    23341 agtataagga tttcaaagcg gcgcagagtg attgctgtat gcaccctggt tcagacctgg
    23401 tagggaaggt tgaaatgact gagtggttag ttgactttgc tgcccagctt cgcaaaggag
    23461 gcaaccagtg agcgaaatta attaccaggc actgcgtgag gcggcggaac gtgcaattcc
    23521 agcaatggaa cgcctgttaa tgttgccagc tgatgatgat ttgttaagtg aacaggaact
    23581 taaagattac ggtgtggata ttgatgcgct caacgccttc aaatttctga ccggaccaga
    23641 aaccgtgctg gcgctgctgg atgaacggga aagaaaccag caatacataa aacgccgcga
    23701 tcaggagaac gaggatattg cgctaacggt agggaagctg agagttgagc ttgaggaagt
    23761 aaaacaacac gctgaagaat tatccgaaac caaggctgtt cgtaaccaat ggcggccaga
    23821 tatttgccca ataaccggac gtgcattttt catgtggatt gagcatccaa cattgggaaa
    23881 tgtgccgaca tatggtggcc cattagatag ttacaccatt ccaacaaagg acggtgacgg
    23941 tgagttttca tgtgagcgtt acgatcatga ttttggcggt tgggtagaaa gcgaatgtct
    24001 tgggttatat ctgattgatg atagagaaca atgcagggtc tacgaactgg aggaacgcgt
    24061 taaggaactg gatgctcggg aaatatcgct cccggaacgt agcagcatgc ttcatcgaac
    24121 agattttcac gatgattacc aaacggtaat ggcatacaaa gtttctgaag tcatcgctgc
    24181 aatccgcgcc gcaggcattc gcatcaaagg aggtgagtaa tgcgtgtggc atgtatcggc
    24241 ttgttaccgt acccgactcg tttttgggct tctgcgctaa ttgcaaagcc acatgtcctg
    24301 atggctgaca acatcatccc ggcaccaaag cgccgccata ccggtattgc agcggcacga
    24361 cgagcagcaa agagacgcag gagagcaaag cgatgaaaaa ccgtaaagca aagattctgt
    24421 tagttcgtag aaacgctcct ggcgtctggc agtgggtgag actcagcaac cgacggatgg
    24481 ggttgatgaa atattacggg atgatggatt gtggtttttg caaaaagccc agcgcggcgc
    24541 aaaaccgctg gaaaaaccac tgttatctgc gcactaaagg agagtgagat ggcgttaaca
    24601 caccgcgaac tctgtcagat tgcgtacaag ttccttaagc gcaacgggtt caaggtttgc
    24661 tttcatgacc gctttatagc tgtaaccagc accggagaac agccagatgc tatgggattc
    24721 agaagttcag catcatgctt gatagaggcg aagtgttctc gtgctgactt gttggcagat
    24781 agaaaaaagc gtttccgtaa aaatccgtct cttggaatgg gcgactggcg attctttatt
    24841 agtgagccgg gaattatttc aattgaggat ttaccacctg gctggggatt acttcacgtt
    24901 gttaacggaa gagtacggaa agtacatggg tggcccaagg gtaattgctg ttggggtaat
    24961 cctgacgata agccatttac tggaaataag caggttgaat gcgattacat gttatctgca
    25021 ttaaggcgca tggagttgag agggcacctt aatgaaatat atgacggtgt aattgttaat
    25081 aagaaagaag gaaacgcggc atgatcacta ttaccaataa gaaacagtat cccagcgagc
    25141 aatatcttaa tgagctgatc accaacatag agtttgctgc aagggcacca gttgaagtcg
    25201 tgagagcaat ggcagcagag ctacagaagc ggcgcgaagc tgatagtgca gaaccagttg
    25261 cttatatttt caaacatccg gccgggaaat tattctgggc tttaacggat gaaagcaata
    25321 aagagcaagc ggacgttatt cctgtttatg ctgccgcgcc tgcgtcggtt gtgccggata
    25381 atgcatcaga gcctcttgct tatgcttaca aagagcttac gcctgagatt atgcgcaacc
    25441 atttagctgt attcgagcga tatggaatag ccccaaacga tagcactacc acaattcagg
    25501 cactgcgaat cgcgctggat ggtatagagc ggagcgacgc catgcttcag ggtaaaggag
    25561 ggtgatatgg ctattgccgc aagttacaca atgcatctct attgtgactg ccgccagtgt
    25621 acggaaggtg tatatccagt gccagacttc ggtgagtata tcggtacgtc atgggctggc
    25681 tgtgcaaaag aggcgcgcaa ggatggctgg cgaataagca aagacaaaac gcgtgctttt
    25741 gcgcccgggc ataaagtttt gaggattaac aaatgaccac tattaccaaa gagcgactgc
    25801 tgacaatcaa gcagtggcgc gaaacatacg gaccgggtag caacgttgta ctgccagcag
    25861 aagaagcgga agaactggca cggattgcac tggcatcgct ggaagcagat cccgttaaac
    25921 gagttaactc agatcagatg caccgagtct gcttagaagc taatcgctat ttagataaat
    25981 atgacgcgat ggcgaaagag gtaaataagt tgcttggacg catcgccccg ccagcgccgg
    26041 tattgcctca tgagtggagg ctgtcaaatg cacaggcgtt tatcgagcga tactctccgc
    26101 cttcagtgga agaagccgca ctatttgcct ggaactcctg ccgcgccgcc atgcttcatg
    26161 gtgccgaacc tgtaagccaa acttacaagt tgaaccagct atcgggcaac tctccggcaa
    26221 ctccggatgg ttggataagc tgtagtgagc gaatgccgaa taccaaaaca gccgttcttg
    26281 ttgccgtgga gtttgacagg aaaggtgact ggcgaatgaa atgggctact tacatccccg
    26341 ggcatcctga cgctaatgat gggtggataa ttcctggtgc gtcgtggaaa ccgtcacact
    26401 ggatgccgct accagaaccg ccgcaggagg tgaatcaatg agctggcctg aagcattcac
    26461 cacggtagga attgtgatgg cggcagcact gggtttgtat tcaatttgtc gctggtggta
    26521 acgatgggaa aaataacttt tgtagtcgaa tttgaggatg gcaaagagcc acctgttagc
    26581 gccaatcttg atgttgctgg tggcaggctg gtttcggttc tatttggtga ttaccgcgac
    26641 gacttctttc agcctgaaga agttgatgta gtgcgagaag cattaaacga gctaagtgtt
    26701 gataacgatg atgctcatgc ggaaatcatc aaaaaaatgg aactgctaac tcactaaatt
    26761 atcaattatg gtgctatcac ctacgacacc gagagaaaat ttataatgtc aaaagtaaat
    26821 gttttgattt tttcagcaat tgttggcttt ggttttactg ccggagtgca gatttatatt
    26881 gcgtgggaaa aaatcatcaa ctacgcatgg agttgtttta ttaagtgagg taagtatgtg
    26941 gagaggtaat agtcatggca aaagccagat gatacttacc gaatataagc tagaccacaa
    27001 aaccaataaa tcacgctcag tatatttgct ccggcacaat agccgcgtaa gaaataccgt
    27061 gctggaacaa aatctgacag ttgaaatgga taattacggt ggattcaagc caacaatttc
    27121 gcttgatgat tttcctcgtg gtttaagcga aagagaagca atgctgaaat tagcagaatg
    27181 gctacaaaga ttaagcattg ctattgaaga taactggtct gaaccttaaa tttatatgat
    27241 gacactaaaa cattttcttg accgcccatt atgggcggca gccgcaggct atgactttaa
    27301 ttatatggat tgcatgtctt atactgccaa tgcatacgac cattcgttca gcctgctgtt
    27361 taattcttta agaatattgc cggaaacaga agttggagag cttcatttat ggatattggg
    27421 ctttatcgcg gctgtcgttg gtattgctgt atggcctttt attttctggc tggtggctgt
    27481 tgtagtgtgg tttaagtgca agacataccg gagaaagtat ttcttaggtg atggaatgac
    27541 cgatattgcc aaaatgaaca ttgaaaaatg gactaaggaa tgtgaaaaga aatggcgcaa
    27601 aaagaaatga ctactctaac gacagcatac ttacagcaat tggtattttt tgcaggcgag
    27661 gctacttgtc atcctgacgc aaactattta ttggaatttg agaggttagc gtcacccggt
    27721 atcgttctgg aactggccca acaggtgctg gccttaagac aaaaagagca acatgaaagt
    27781 aatacgtgta gattgaattt tgagcagtgg ctggaacagc aacgcggaaa aatcgatgtg
    27841 gactgtggtt gtgtgtctac tgaaacattc atgcactggc tgcgggtagc ttacgaggct
    27901 ggcaactatc cggatattcc ggatagttcg gtgccagcgc caggaaaggg cgtcaccggt
    27961 gaacgtatcc gcattaagcc gcatgtttat cgcgaactgg ttaaccgtct ccacgataca
    28021 gcgatcaagt gtgcgggcac ccagcaatta cgagaaagaa ttagccgtgt tttgggcgac
    28081 gttattacgc cagatcatca taaacaagcc gagaaaagtg acctggaaag gtgtcacctt
    28141 gaggcggcat taaacattaa gccggggcat acgcttggca ttatcgatgc tctgttggtt
    28201 cataagatgg ccagggcttt attgccgctg gtggctgaaa agcatgaggc ggaccatgcc
    28261 aacgaaagct gagttacagg cgcgcataga gattcttgaa aaagagaatg cgagtctaaa
    28321 aggaatgctg gcacgggcgg aaagggaatt atcaggcaaa ttcttgccag aagaactgcc
    28381 accagcagat ataccagatc gagtgtcccg gtggatgaag tcttttggtt taccgtggga
    28441 ggctttttgg tgctacgagc atcgcagatg gtgtgatgaa cttgatagca gtttccccta
    28501 ctttgcggaa gggaacacct gccctgagtg taggaggtag cgcgatgttt cgtcctcgtc
    28561 caataccgcc gaaaataact aatggctatc agccgaatac tccagaaata caggggagcg
    28621 aggggcttaa accaccacca gcaccacgta ttccgcctgt agcaaggaac gtatcattac
    28681 gacgagagtt aatattagcc ttgtcgcccg ttctggctgc tcgccaggat tgggtaagta
    28741 atgatgacct ggcagaaaaa atcacatcgc tggtggataa aattctgcag attggcaatg
    28801 agattacgct gatccattgg tgatgaatag tagcaaagcg cacaaaatca tctgcggtgg
    28861 ttggtgtaca taacgagttt gcaccaaagg tgtctcttta atgtatactg tataaatgaa
    28921 cagtattatt gaggtgaaaa cgctatgggc ttcccttctc ctgcggcgga ttatgttgaa
    28981 agcagaattt ctcttgatca gcaactaatc aggcatccat cagcgaccta cttcatgcgg
    29041 gcggcagaca gccatcaccg tgagggaata ttgcagggtg ctttgctggt ggttgattcc
    29101 tcgcttactc cggttgatgg ttcgctgctt gtctgcgcta tggagggtga atatcgcata
    29161 aagagatacc gaaagtatcc gcgccagcac ctggaggatt taagcaccgg gaagaaagag
    29221 gcgttaccag tagatgacga tggatacacg ggcagtaatg ctgtttttgg tgtgatcacc
    29281 cacattatca atgacgccag gggcggtgaa tttgatgata gtcctgctat atgatttgtt
    29341 ttcagagaaa ccatagagag gcatgactgg atctatatcg ccctggccat ttaaatgatt
    29401 actcccatta gacgcggtta ctcacttgaa gataagatga ggttataatt ctctttttag
    29461 gtacgcgaat gagttcgtct gcttttgtca ctgttgtcac tggagtaagc gtttttgtcc
    29521 taggtcgact catcatcaag ggggttgagt cttacatttc attcaaggaa cagttggggg
    29581 aaatatcgca tctgttattg tctaaccaag caaagctatc gaatccccgc tctgacttaa
    29641 aacaagaaat catccatgat ttgaaagatg cggcagcaca gttacgggtg aagtacgaac
    29701 tattaccttt ctatatcaaa atgttacaca tcggttttcg ccttgttcca tcagaaactg
    29761 aaattcgtaa agcggcgcaa gaattgaatc tgatagcctc gatacatgac ggtaaaaccg
    29821 gagagctgcc atatgagcac atagccaaga ttggattact gctcagaatt ccaaccactt
    29881 attctgctta atcccagcaa ttgcaagact gcatctcgag gatttaaggc accaagaagt
    29941 ggtattcagc atcattaaaa cctataattg cgtacacata acgagcacac gaggtgttta
    30001 tgcaatccat taacttccgt accgcgcgcg gcaacctttc tgaagtgctc aacaatgttg
    30061 aagccgggga agaggttgaa atcacccgca gaggccgtga gccagcagta attgttagca
    30121 aggctacttt tgaagcctac aaaaaagcgg cgctggatgc cgaatttgca tccctgtttg
    30181 acaccctgga ctccaccaac aaggaactgg ttaaccgata atgaggcata tatcaccgga
    30241 agaacttatt gcgcttcatg atgcgaatat aagccgctac ggtggcctgc cgggaatgtc
    30301 agatccgggt agggcagagg ccattatcgg gagagttcag gccagagttg cctacgaaga
    30361 gatcagcgac cttttcgaag tctccgccac ctacctggtg gctacagcga gagggcatat
    30421 attcaatgat gccaataagc gtaccgcgct aaacagtgcg ctgttatttc tacgccgtaa
    30481 cggggtgcag gtatttgatt cacctgaact ggcagacctt accgtagggg ctgcgactgg
    30541 cgagatatct gtatcttctg tcgccgccac gttacgtaga ttgtatggtt ctgcggagta
    30601 gattaatggc acgcaaatac aacaaattgt cccgtgaagc gttaaagatg cttcttgatg
    30661 gcgtgagtcg ccgcgaggta aagcaatacc tggttggtaa gcaaattgga gccaggactg
    30721 ctattgctgt gttatgccgt caggaaatgg ttgtgcttaa acagagaatg ccgggcagca
    30781 gataaagccc aatcagtgat gaaaggtgtg atgtgaaagc cgtaattact ccctttgtac
    30841 aaaaagagct tggcgtcgcc acattcaaag tggatcagga agtcagaaag ctggtggagg
    30901 ctggccgtaa atttattatg gagccggtgc cgcgtgagtt aatcgagcac atggacgacg
    30961 gcctcgttgt ttccgagcaa actatggcaa caaatgaggc gttgcagccg ttttttaaca
    31021 gcgatgaact gtttcgccgt attggtggaa ttgacgcgct ggtggcgtgg ttgcgcagga
    31081 aagaggggca atgccaggcc gcagatcgta gctggtgtga caaccatatt gtccacgcag
    31141 aacgagacaa tagcgcggtg ttgttgtgct ggcatcacga taaccattac cggatgcgtg
    31201 gttttaatga gctgaaagaa acgctgcata ataatcgcgt taactggata ctggatgtcg
    31261 cccgtcagga aatggggctt tcagatggcc atgatttaag tattcaggaa ctgtgctggt
    31321 gggctttcat gcgcaacatg atgcacctga tgccggaaga agtttgccgt atatcaataa
    31381 ataagatgaa agccgcaacg caggatagcg gacctctgaa agaggcggat attcgcccgt
    31441 atgacgatcg cgctacagca tatgttcaga tgatggaaga acgcgccgcg ccgatgcgtg
    31501 caaaagtatg ccctgtggat gttgactccg accctggcat ggcgcatttc aaaataccaa
    31561 aactgcaatc gctaaaatta cctgagtaca tggactttgt tgcttcccgt ccatgctgtg
    31621 ggtgtggagc tgcgggagct ggcgctcaca ttacgcctta tatcgttcgt catagtcgat
    31681 tatgcgcgca tgacatttac gcaattcctc tgtgccagtc atgccagcgt gatattgagc
    31741 gtgaccgcga taattgggag aagacgcacg gtaggctggc gatgcatcaa cga
//
LOCUS       DAZGTR010000046        31541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGTR010000046 DAZGTR010000000
VERSION     DAZGTR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.046"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..702
                     /locus_tag="ACHLCY_002311"
     CDS             <1..702
                     /locus_tag="ACHLCY_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP0369559.1"
                     /translation="DFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPER
                     AIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFR
                     KLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHT
                     PFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIRE
                     GGRTVGAGVVAKVLS"
     gene            932..1315
                     /gene="secE"
                     /locus_tag="ACHLCY_002312"
     CDS             932..1315
                     /gene="secE"
                     /locus_tag="ACHLCY_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP0369560.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1317..1862
                     /gene="nusG"
                     /locus_tag="ACHLCY_002313"
     CDS             1317..1862
                     /gene="nusG"
                     /locus_tag="ACHLCY_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP0369561.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2021..2449
                     /gene="rplK"
                     /locus_tag="ACHLCY_002314"
     CDS             2021..2449
                     /gene="rplK"
                     /locus_tag="ACHLCY_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP0369562.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2453..3157
                     /gene="rplA"
                     /locus_tag="ACHLCY_002315"
     CDS             2453..3157
                     /gene="rplA"
                     /locus_tag="ACHLCY_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP0369563.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3570..4067
                     /gene="rplJ"
                     /locus_tag="ACHLCY_002316"
     CDS             3570..4067
                     /gene="rplJ"
                     /locus_tag="ACHLCY_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP0369564.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4134..4499
                     /gene="rplL"
                     /locus_tag="ACHLCY_002317"
     CDS             4134..4499
                     /gene="rplL"
                     /locus_tag="ACHLCY_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP0369565.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4819..8847
                     /gene="rpoB"
                     /locus_tag="ACHLCY_002318"
     CDS             4819..8847
                     /gene="rpoB"
                     /locus_tag="ACHLCY_002318"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP0369566.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8924..13147
                     /gene="rpoC"
                     /locus_tag="ACHLCY_002319"
     CDS             8924..13147
                     /gene="rpoC"
                     /locus_tag="ACHLCY_002319"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP0369567.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVIVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13360..13897
                     /gene="yjaZ"
                     /locus_tag="ACHLCY_002320"
                     /pseudo
     CDS             13360..13897
                     /gene="yjaZ"
                     /locus_tag="ACHLCY_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
     gene            complement(14307..15440)
                     /gene="thiH"
                     /locus_tag="ACHLCY_002321"
     CDS             complement(14307..15440)
                     /gene="thiH"
                     /locus_tag="ACHLCY_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP0369568.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15437..16207)
                     /gene="thiG"
                     /locus_tag="ACHLCY_002322"
     CDS             complement(15437..16207)
                     /gene="thiG"
                     /locus_tag="ACHLCY_002322"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP0369569.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16209..16409)
                     /gene="thiS"
                     /locus_tag="ACHLCY_002323"
     CDS             complement(16209..16409)
                     /gene="thiS"
                     /locus_tag="ACHLCY_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP0369570.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16393..17148)
                     /gene="thiF"
                     /locus_tag="ACHLCY_002324"
     CDS             complement(16393..17148)
                     /gene="thiF"
                     /locus_tag="ACHLCY_002324"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP0369571.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(17141..17776)
                     /gene="thiE"
                     /locus_tag="ACHLCY_002325"
     CDS             complement(17141..17776)
                     /gene="thiE"
                     /locus_tag="ACHLCY_002325"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP0369572.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17776..19671)
                     /gene="thiC"
                     /locus_tag="ACHLCY_002326"
     CDS             complement(17776..19671)
                     /gene="thiC"
                     /locus_tag="ACHLCY_002326"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP0369573.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19752..19852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19904..20380)
                     /gene="rsd"
                     /locus_tag="ACHLCY_002327"
     CDS             complement(19904..20380)
                     /gene="rsd"
                     /locus_tag="ACHLCY_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP0369574.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20475..21248
                     /gene="nudC"
                     /locus_tag="ACHLCY_002328"
     CDS             20475..21248
                     /gene="nudC"
                     /locus_tag="ACHLCY_002328"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP0369575.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21288..22352
                     /gene="hemE"
                     /locus_tag="ACHLCY_002329"
     CDS             21288..22352
                     /gene="hemE"
                     /locus_tag="ACHLCY_002329"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP0369576.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVSLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22362..23033
                     /gene="nfi"
                     /locus_tag="ACHLCY_002330"
     CDS             22362..23033
                     /gene="nfi"
                     /locus_tag="ACHLCY_002330"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP0369577.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            23076..23666
                     /gene="yjaG"
                     /locus_tag="ACHLCY_002331"
     CDS             23076..23666
                     /gene="yjaG"
                     /locus_tag="ACHLCY_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP0369578.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGDSNIGIIFQQ"
     gene            23853..24125
                     /gene="hupA"
                     /locus_tag="ACHLCY_002332"
     CDS             23853..24125
                     /gene="hupA"
                     /locus_tag="ACHLCY_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP0369579.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24138..24833
                     /gene="yjaH"
                     /locus_tag="ACHLCY_002333"
     CDS             24138..24833
                     /gene="yjaH"
                     /locus_tag="ACHLCY_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP0369580.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24835..25260)
                     /gene="zraP"
                     /locus_tag="ACHLCY_002334"
     CDS             complement(24835..25260)
                     /gene="zraP"
                     /locus_tag="ACHLCY_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP0369581.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(25263..25391)
                     /locus_tag="ACHLCY_002335"
     CDS             complement(25263..25391)
                     /locus_tag="ACHLCY_002335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369582.1"
                     /translation="MTRYPQQASHFYSFIMPDYPSYQRFIVGTEDAIPEVRQPLED"
     gene            25498..26874
                     /gene="zraS"
                     /locus_tag="ACHLCY_002336"
     CDS             25498..26874
                     /gene="zraS"
                     /locus_tag="ACHLCY_002336"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP0369583.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26871..28196
                     /gene="zraR"
                     /locus_tag="ACHLCY_002337"
     CDS             26871..28196
                     /gene="zraR"
                     /locus_tag="ACHLCY_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP0369584.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCVALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28193..29482)
                     /gene="purD"
                     /locus_tag="ACHLCY_002338"
     CDS             complement(28193..29482)
                     /gene="purD"
                     /locus_tag="ACHLCY_002338"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP0369585.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKVFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29494..31083)
                     /gene="purH"
                     /locus_tag="ACHLCY_002339"
     CDS             complement(29494..31083)
                     /gene="purH"
                     /locus_tag="ACHLCY_002339"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP0369586.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7440 a         8167 c         8638 g         7296 t
ORIGIN      
        1 gacttcccgg gcgacgacac tccgatcgtt cgtggttctg ctctgaaagc gctggaaggc
       61 gacgcagagt gggaagcgaa aatcctggaa ctggctggct tcctggattc ttacattccg
      121 gaaccagagc gtgcgattga caagccgttc ctgctgccga tcgaagacgt attctccatc
      181 tccggtcgtg gtaccgttgt taccggtcgt gtagaacgcg gtatcatcaa agttggtgaa
      241 gaagttgaaa tcgttggtat caaagagact cagaagtcta cctgtactgg cgttgaaatg
      301 ttccgcaaac tgctggacga aggccgtgct ggtgagaacg taggtgttct gctgcgtggt
      361 atcaaacgtg aagaaatcga acgtggtcag gtactggcta agccgggcac catcaagccg
      421 cacaccaagt tcgaatctga agtgtacatt ctgtccaaag atgaaggcgg tcgtcatact
      481 ccgttcttca aaggctaccg tccgcagttc tacttccgta ctactgacgt gactggtacc
      541 atcgaactgc cggaaggcgt agagatggta atgccgggcg acaacatcaa aatggttgtt
      601 accctgatcc acccgatcgc gatggacgac ggtctgcgtt tcgcaatccg tgaaggcggc
      661 cgtaccgttg gcgcgggcgt tgtagcaaaa gttctgagct aattgccgat aacatttgac
      721 gcaatgcgca ctaaaagggc atcatttgat gccctttttg cacgctttcg taccagaacc
      781 tggctcatca gtgattttct ttgtcataat cattgctgag acaggctctg ttgagggcgt
      841 ataatccgaa aagctaatac gcgtttcgat ttggtttgcc tcgcgatcgc ggggtgaaaa
      901 tgtttgtaga aaacttctga caggttggtt tatgagtgcg aataccgaag ctcaaggaag
      961 cgggcgcggc ctggaagcga tgaagtgggt cgttgtggtg gcattgctcc tggtggcgat
     1021 tgtcggcaac tacctttatc gcgacattat gctgccgctg cgtgcgctgg ccgtagtaat
     1081 tctgattgct gcagcgggtg gtgtcgcgct gttaacgaca aaaggtaaag ctaccgttgc
     1141 ttttgcccgt gaagcgcgta ccgaagtccg taaggtcatt tggccgactc gccaggaaac
     1201 attgcacacc acgctgattg tggctgcggt taccgcagta atgtcactga tcctgtgggg
     1261 actggatggt attctggttc gcctggtatc ctttatcact ggcctgaggt tctgagatgt
     1321 ctgaagctcc taaaaagcgc tggtacgtcg ttcaggcgtt ttccggtttt gaaggccgcg
     1381 tagcaacgtc gctgcgtgag catatcaaat tacacaacat ggaagatttg tttggtgaag
     1441 tcatggtacc aaccgaagaa gtggttgaaa tccgtggcgg tcagcgtcgc aaaagcgaac
     1501 gtaaattctt ccctggctac gtcctcgttc agatggtgat gaacgacgcg agctggcacc
     1561 tggtgcgcag cgtaccgcgt gtgatgggct tcatcggcgg tacttccgat cgtcctgcgc
     1621 caatcagcga taaagaagtc gatgcgatta tgaaccgcct gcagcaggtt ggtgataagc
     1681 cgcgtccgaa aacgctgttt gaaccgggtg aaatggtccg tgttaatgat ggtccgttcg
     1741 ctgacttcaa cggtgttgtt gaagaagtgg attacgagaa atctcgtctg aaagtgtctg
     1801 tttctatctt cggtcgtgcg accccggtag agctggactt cagccaggtt gaaaaagcct
     1861 aacccagcga tcaaaaaagc ggcgatttaa tcgttgcaca aggcgtgaga ttggaataca
     1921 atttcgcgcc ttttgttttt atgggccttg cccgtaaaac gattttttat atcacgggga
     1981 gcctctcaga ggcgttatta cccaacttga ggaatttata atggctaaga aagtacaagc
     2041 ctatgtcaag ctgcaggttg cagctggtat ggctaacccg agtccgccag taggtccggc
     2101 tctgggtcag cagggcgtaa acatcatgga attctgcaaa gcgttcaacg caaaaactga
     2161 ttccatcgaa aaaggtctgc cgattccggt agtaatcacc gtttacgctg accgttcttt
     2221 cactttcgtt accaagaccc cgccggcagc agttctgctg aaaaaagcgg ctggtatcaa
     2281 gtctggttcc ggtaagccga acaaagacaa agtgggtaaa atttcccgcg ctcagctgca
     2341 ggaaatcgcg cagaccaaag ctgccgacat gactggtgcc gacattgaag cgatgactcg
     2401 ctccatcgaa ggtactgcac gttccatggg cctggtagtg gaggactaag aaatggctaa
     2461 actgaccaag cgcatgcgtg ttatccgcga gaaagttgat gcaaccaaac agtacgacat
     2521 caacgaagct atcgcactgc tgaaagagct ggcgactgct aaattcgtag aaagcgtgga
     2581 cgtagctgtt aacctcggca tcgacgctcg taaatctgac cagaacgtac gtggtgcaac
     2641 tgtactgccg cacggtactg gccgttccgt tcgcgtagcc gtatttaccc aaggtgcaaa
     2701 cgctgaagct gctaaagctg caggcgcaga actggtaggt atggaagatc tggctgacca
     2761 gatcaagaaa ggcgaaatga actttgacgt tgttattgct tctccggatg caatgcgcgt
     2821 tgttggccag ctgggccagg ttctgggtcc gcgcggcctg atgccaaacc cgaaagtggg
     2881 tactgtaaca ccgaacgttg ctgaagcggt taaaaacgct aaagctggcc aggttcgtta
     2941 ccgtaacgac aaaaacggca tcatccacac caccatcggt aaagtggact ttgacgctga
     3001 caaactgaaa gaaaacctgg aagctctgct ggttgcgctg aaaaaagcaa aaccgactca
     3061 ggcgaaaggc gtgtacatca agaaagttag catctccacc accatgggtg caggtgttgc
     3121 agttgaccag gctggcctga gcgcttctgt aaactaatgc ctttacgtgg gcggtgattt
     3181 tgtctacaat cttaccccca cgtataatgc ttaatgcaga cgtatatccg agatattcgg
     3241 gttgtggcaa ggcggcaact gagtgagtcg ccaggagcat agctaactat gtgactggtg
     3301 cgaatgaagg aagccaacgc cgccacaagt tgaatagcga cggatagaaa agatttgttc
     3361 gttggagcct ggcctatcca ggcctccgtc gaagaccgca ggagtttcgc aagaaactta
     3421 atcccctgcg tagacggtga cagaacgcta agattattct tttatattct ggcttgtttc
     3481 tgctcaccgt aattaagacg ctctctccgt ttggaggagt gaagtgagtt ccagagattt
     3541 tctctggcaa acatccagga gcaaagctaa tggctttaaa tcttcaagac aaacaagcga
     3601 ttgttgctga agtcagcgaa gtagccaaag gcgcgctgtc tgcagtagtt gcggattccc
     3661 gtggcgtaac tgtagataaa atgactgaac tgcgtaaagc aggtcgcgaa gctggcgtat
     3721 acatgcgtgt tgttcgtaac accctgctgc gccgtgctgt tgaaggtact ccgttcgagt
     3781 gcctgaaaga cgcgtttgtt ggtccgaccc tgattgcata ctctatggaa cacccgggcg
     3841 ctgctgctcg tctgttcaaa gagttcgcga aagcgaatgc aaaatttgag gtcaaagccg
     3901 ctgcctttga aggtgagctg atcccggcgt ctcagatcga ccgcctggca actctgccga
     3961 cctacgaaga agcaattgca cgcctgatgg caaccatgaa agaagcttcg gctggcaaac
     4021 tggttcgtac tctggctgct gtacgcgatg cgaaagaagc tgcttaatcg cagttatctt
     4081 tttaacgcat tcgcttacgt ataaacttat tctgatattc aggaacaatt taaatgtcta
     4141 tcactaaaga tcaaatcatt gaagcagttg cagctatgtc tgtaatggac gttgtagaac
     4201 tgatctctgc aatggaagaa aaattcggtg tttccgctgc tgctgctgta gctgtagctg
     4261 ctggcccggt tgaagctgct gaagaaaaaa ctgaattcga cgtaattctg aaagctgctg
     4321 gcgctaacaa agttgctgtt atcaaagcag tacgtggcgc aactggcctg ggtctgaaag
     4381 aagctaaaga cctggtagaa tctgcaccgg ctgctctgaa agaaggcgtg agcaaagacg
     4441 acgcagaagc actgaaaaaa gctctggaag aagctggcgc tgaagttgaa gttaaataag
     4501 ccaacccttc cggttgcagc ctgagaaatc aggctgatgg ctggtgactt tttagtcacc
     4561 agcctttttg cgctgtaagg cgccagtagc gtttcacact gtttgactac tgctgtgcct
     4621 ttcaatgctt gtttctatcg acgacttaat atactgcgac aggacgtccg ttctgtgtaa
     4681 atcgcaatga aatggtttaa gcgtgatagc aacaggcatt gcggaaagtg ttccattttc
     4741 cggtcaacaa aatagtgttg cacaaactgt ccgctcaatg gacagatggg tcgacttgtc
     4801 agcgagctga ggaaccctat ggtttactcc tataccgaga aaaaacgtat tcgtaaggat
     4861 tttggtaaac gtccacaagt tctggatgta ccttatctcc tttctatcca gcttgactcg
     4921 tttcagaaat ttatcgagca agatcctgaa gggcagtatg gtctggaagc tgctttccgt
     4981 tccgtattcc cgattcagag ctacagcggt aattccgagc tgcaatacgt cagctaccgc
     5041 cttggcgaac cggtgtttga cgtccaggaa tgtcaaatcc gtggcgtgac ctattccgca
     5101 ccgctgcgcg ttaaactgcg tctggtgatc tatgagcgcg aagcgccgga aggcaccgta
     5161 aaagacatta aagaacaaga agtctacatg ggcgaaattc cgctcatgac agacaacggt
     5221 acctttgtta tcaacggtac tgagcgtgtt atcgtttccc agctgcaccg tagtccgggc
     5281 gtcttctttg actccgacaa aggtaaaacc cactcttcgg gtaaagtgct gtataacgcg
     5341 cgcatcatcc cttaccgtgg ttcctggctg gacttcgaat tcgatccgaa ggacaacctg
     5401 ttcgtacgta tcgaccgtcg ccgtaaactg cctgcgacca tcattctgcg cgccctgaac
     5461 tacaccacag agcagatcct cgacctgttc tttgaaaaag ttatctttga aatccgtgat
     5521 aacaagctgc agatggaact ggtgccggaa cgcctgcgtg gtgaaaccgc atcttttgac
     5581 atcgaagcta acggtaaagt gtacgtagaa aaaggccgcc gtatcactgc gcgccacatt
     5641 cgccagctgg aaaaagacga cgtcaaactg atcgaagtcc cggttgagta catcgcaggt
     5701 aaagtggttg ctaaagacta tattgatgag tctaccggcg agctgatctg cgcagcgaac
     5761 atggagctga gcctggatct gctggctaag ctgagccagt ctggtcacaa gcgtatcgaa
     5821 acgctgttca ccaacgatct ggatcacggc ccatatatct ctgaaacctt acgtgtcgac
     5881 ccaactaacg accgtctgag cgcactggta gaaatctacc gcatgatgcg ccctggcgag
     5941 ccgccgactc gtgaagcagc tgaaagcctg ttcgagaacc tgttcttctc cgaagaccgt
     6001 tatgacttgt ctgcggttgg tcgtatgaag ttcaaccgtt ctctgctgcg cgaagaaatc
     6061 gaaggttccg gtatcctgag caaagacgac atcattgatg ttatgaaaaa gctcatcgat
     6121 atccgtaacg gtaaaggcga agtcgatgat atcgaccacc tcggcaaccg tcgtatccgt
     6181 tccgttggcg aaatggcgga aaaccagttc cgcgttggcc tggtacgtgt agagcgtgcg
     6241 gtgaaagagc gtctgtctct gggcgatctg gataccctga tgcctcagga tatgatcaac
     6301 gccaagccga tttccgcagc agtgaaagag ttcttcggtt ccagccagct gtctcagttt
     6361 atggaccaga acaacccgct gtctgagatt acgcacaaac gtcgtatctc cgcactcggc
     6421 ccaggcggtc tgacccgtga acgtgcaggc ttcgaagttc gagacgtaca cccgactcac
     6481 tacggtcgcg tatgtccaat cgaaacccct gaaggtccga acatcggtct gatcaactct
     6541 ctgtccgtgt acgcacagac taacgaatac ggcttccttg agactccgta tcgtaaagtg
     6601 accgacggtg ttgtaactga cgaaattcac tacctgtctg ctatcgaaga aggcaactac
     6661 gttatcgccc aggcgaactc caacctggat gaagaaggcc acttcgtaga agacctggta
     6721 acttgccgta gcaaaggcga atccagcttg ttcagccgcg accaggttga ctacatggac
     6781 gtatccaccc agcaggtggt atccgtcggt gcgtccctga tcccgttcct ggaacacgat
     6841 gacgccaacc gtgcattgat gggtgcgaac atgcaacgtc aggccgttcc gactctgcgc
     6901 gctgataagc cgctggttgg tactggtatg gaacgtgctg ttgccgttga ctccggtgta
     6961 actgcggtag ctaaacgtgg tggtgtcgtt cagtacgtgg atgcttcccg tatcgttatc
     7021 aaagttaacg aagacgagat gtatccgggt gaagcaggta tcgacatcta caacctgacc
     7081 aaatacaccc gttctaacca gaacacctgt atcaaccaga tgccgtgtgt gtctctgggt
     7141 gaaccggttg aacgtggcga cgtgctggca gacggtccgt ccaccgacct cggtgaactg
     7201 gcgcttggtc agaacatgcg cgtagcgttc atgccgtgga atggttacaa cttcgaagac
     7261 tccatcctcg tatccgagcg tgttgttcag gaagaccgtt tcactaccat ccacattcag
     7321 gaactggcgt gtgtgtcccg tgacaccaag ctggggccgg aagagatcac cgctgacatc
     7381 ccgaacgtgg gtgaagctgc gctctccaaa ctggatgaat ccggtatcgt ttacattggt
     7441 gcggaagtga ccggtggcga cattctggtt ggtaaggtaa cgccgaaagg tgaaactcag
     7501 ctgaccccag aagaaaaact gctgcgtgcg atcttcggtg agaaagcctc tgacgttaaa
     7561 gactcttctc tgcgcgtacc aaacggtgta tccggtacgg ttatcgacgt tcaggtcttt
     7621 actcgcgatg gcgtagaaaa agacaaacgt gcgctggaaa tcgaagaaat gcagctcaaa
     7681 caggcgaaga aagacctgtc tgaagaactg cagatcctcg aagcgggtct gttcagccgt
     7741 atccgtgctg tgctggtagc cggtggcgtt gaagctgaga agctcgacaa actgccgcgc
     7801 gatcgctggc tggagctggg cctgacagac gaagagaaac aaaatcagct ggaacagctg
     7861 gctgagcagt atgacgaact gaaacacgag ttcgagaaga aactcgaagc gaaacgccgc
     7921 aaaatcaccc agggcgacga tctggcaccg ggcgtgctga agattgttaa ggtatatctg
     7981 gcggttaaac gccgtatcca gcctggtgac aagatggcag gtcgtcacgg taacaagggt
     8041 gtaatttcta agatcaaccc gatcgaagat atgccttacg atgaaaacgg tacgccggta
     8101 gacatcgtac tgaacccgct gggcgtaccg tctcgtatga acatcggtca gatcctcgaa
     8161 acccacctgg gtatggctgc gaaaggtatc ggcgacaaga tcaacgccat gctgaaacag
     8221 cagcaagaag tcgcgaaact gcgcgaattc atccagcgtg cgtacgatct gggcgctgac
     8281 gttcgtcaga aagttgacct gagtaccttc agcgatgaag aagttatgcg tctggctgaa
     8341 aacctgcgca aaggtatgcc aatcgcaacg ccggtgttcg acggtgcgaa agaagcagaa
     8401 attaaagagc tgctgaaact tggcgacctg ccgacttccg gtcagatccg cctgtacgat
     8461 ggtcgcactg gtgaacagtt cgagcgtccg gtaaccgttg gttacatgta catgctgaaa
     8521 ctgaaccacc tggtcgacga caagatgcac gcgcgttcca ccggttctta cagcctggtt
     8581 actcagcagc cgctgggtgg taaggcacag ttcggtggtc agcgtttcgg ggagatggaa
     8641 gtgtgggcgc tggaagcata cggcgcagca tacaccctgc aggaaatgct caccgttaag
     8701 tctgatgacg tgaacggtcg taccaagatg tataaaaaca tcgtggacgg caaccatcag
     8761 atggagccgg gcatgccaga atccttcaac gtattgttga aagagattcg ttcgctgggt
     8821 atcaacatcg aactggaaga cgagtaattc tcgctcaaac aggtcactgc tgtcggggta
     8881 aaacccggca gcggattgtg ctaactccga cgggagcaaa tccgtgaaag atttattaaa
     8941 gtttctgaaa gcgcagacta aaaccgaaga gtttgatgcg atcaaaattg ctctggcttc
     9001 gccagacatg atccgttcat ggtctttcgg tgaagttaaa aagccggaaa ccatcaacta
     9061 ccgtacgttc aaaccagaac gtgacggcct tttctgcgcc cgtatctttg ggccggtaaa
     9121 agattacgag tgcctgtgcg gtaagtacaa gcgcctgaaa caccgtggcg tcatctgtga
     9181 gaagtgcggc gttgaagtga cccagactaa agtacgccgt gagcgtatgg gccacatcga
     9241 actggcttcc ccgactgcgc acatctggtt cctgaaatcg ctgccgtccc gtatcggtct
     9301 gctgctcgat atgccgctgc gcgatatcga acgcgtactg tactttgaat cctatgtggt
     9361 tatcgaaggc ggtatgacca acctggaacg tcagcagatc ctgactgaag agcagtatct
     9421 ggacgcgctg gaagagttcg gtgacgaatt cgacgcgaag atgggtgcgg aagcaatcca
     9481 ggctctgctg aagagcatgg atctggagca agagtgcgaa cagctgcgtg aagagctgaa
     9541 cgaaaccaac tccgaaacca agcgtaaaaa gctgaccaag cgtatcaaac tgctggaagc
     9601 gttcgttcag tctggtaaca aaccagagtg gatgatcctg accgttctgc cggtactgcc
     9661 gccagatctg cgtccgctgg ttccgctgga tggtggtcgt ttcgcgactt ctgacctgaa
     9721 cgatctgtat cgtcgcgtca ttaaccgtaa caaccgtctg aaacgtctgc tggatctggc
     9781 tgcgccggac atcatcgtac gtaacgaaaa acgtatgctg caggaagcgg tagacgccct
     9841 gctggataac ggtcgtcgcg gtcgtgcgat caccggttct aacaagcgtc ctctgaaatc
     9901 tttggccgac atgatcaaag gtaaacaggg tcgtttccgt cagaacctgc tcggtaagcg
     9961 tgttgactac tccggtcgtt ctgtaatcac cgtaggtcca tacctgcgtc tgcatcagtg
    10021 cggtctgccg aagaaaatgg cgctggagct gttcaaaccg ttcatctacg gcaagctgga
    10081 actgcgtggt cttgctacca ccattaaagc tgcgaagaaa atggttgagc gcgaagaagc
    10141 tgtcgtttgg gatatcctgg acgaagttat ccgcgaacac ccggtactgc tgaaccgtgc
    10201 accgactctg caccgtctgg gtatccaggc atttgaaccg gtactgatcg aaggtaaagc
    10261 tatccagctg cacccgctgg tttgtgcggc atataacgcc gacttcgatg gtgaccagat
    10321 ggctgttcac gtaccgctga cgctggaagc ccagctggaa gcgcgtgcgc tgatgatgtc
    10381 taccaacaac atcctgtccc cggcgaacgg cgaaccaatc atcgttccgt ctcaggacgt
    10441 tgttctgggt ctgtactata tgacccgtga ctgtgttaac gccaaaggcg aaggcatggt
    10501 gctgactggc ccgaaagaag cagaacgtct gtatcgctct ggtctggctt ctctgcatgc
    10561 gcgcgttaaa gtgcgtatca ccgagtatga aaaagatgca aacggtgaat tagtagcgaa
    10621 aaccagcctg aaagacacga ctgttggccg tgccattctg tggatgattg taccgaaagg
    10681 tctgccttac tccatcgtca accaggcgct gggtaaaaaa gcaatctcca aaatgctgaa
    10741 cacctgctac cgcattctcg gtctgaaacc gaccgttatt tttgcggacc agatcatgta
    10801 caccggcttc gcctatgcag cgcgttctgg tgcatctgtt ggtatcgatg acatggtcat
    10861 cccggagaag aaacacgaaa tcatctccga ggcagaagca gaagttgctg aaattcagga
    10921 gcagttccag tctggtctgg taactgcggg cgaacgctac aacaaagtta tcgatatctg
    10981 ggctgcggcg aacgatcgtg tatccaaagc gatgatggat aacctgcaaa ctgaaaccgt
    11041 gattaaccgt gacggtcagg aagagaagca ggtttccttc aacagcatct acatgatggc
    11101 cgactccggt gcgcgtggtt ctgcggcaca gattcgtcag cttgctggta tgcgtggtct
    11161 gatggcgaag ccggatggct ccatcatcga aacgccaatc accgcgaact tccgtgaagg
    11221 tctgaacgta ctccagtact tcatctccac ccacggtgct cgtaaaggtc tggcggatac
    11281 cgcactgaaa actgcgaact ccggttacct gactcgtcgt ctggttgacg tggcgcagga
    11341 cctggtggtt accgaagacg attgtggtac ccatgaaggt atcatgatga ctccggttat
    11401 cgagggtggt gacgttaaag agccgctgcg cgatcgcgta ctgggtcgtg taactgctga
    11461 agacgttctg aagccgggta ctgctgatat cctcgttccg cgcaacacgc tgctgcacga
    11521 acagtggtgt gacctgctgg aagagaactc tgtcgacgcg gtaatagtac gttctgttgt
    11581 atcttgtgac accgactttg gtgtatgtgc gcactgctac ggtcgtgacc tggcgcgtgg
    11641 ccacatcatc aacaaaggtg aagcaatcgg tgttatcgcg gcacagtcca tcggtgaacc
    11701 gggtacacag ctgaccatgc gtacgttcca catcggtggt gcggcatctc gtgcggctgc
    11761 tgaatccagc atccaagtga aaaacaaagg tagcatcaag ctcagcaacg tgaagtcggt
    11821 tgtgaactcc agcggtaaac tggttatcac ttcccgtaat actgaactga aactgatcga
    11881 cgaattcggt cgtaccaaag aaagctacaa agtaccttac ggtgcggtac tggcgaaagg
    11941 cgatggcgaa caggttgctg gcggcgaaac cgttgcaaac tgggacccgc acaccatgcc
    12001 ggttatcacc gaagtaagcg gttttgtacg ctttactgac atgatcgacg gtcagaccat
    12061 tacgcgtcag acagacgaat tgaccggtct gtcttcgctg gtggttctgg attccgcaga
    12121 acgtaccgca ggtggtaaag atctgcgtcc ggcactgaaa atcgttgatg ctcagggtaa
    12181 cgacgttctg attccgggta ccgatatgcc tgcgcagtac ttcctgccgg gtaaagcgat
    12241 tgttcagctg gaagatggcg tacagatcag ctctggtgac accctggcgc gtattccgca
    12301 ggaatccggc ggtaccaagg acatcaccgg tggtctgccg cgcgttgcgg acctgttcga
    12361 agcacgtcgt ccgaaagagc cggcaatcct ggctgaaatc agcggtatcg tttccttcgg
    12421 taaagaaacc aaaggtaaac gtcgtctggt tatcaccccg gtagacggta gcgatccgta
    12481 cgaagagatg attccgaaat ggcgtcagct caacgtgttc gaaggtgaac gtgtagaacg
    12541 tggtgacgta atttccgacg gtccggaagc gccgcacgac attctgcgtc tgcgtggtgt
    12601 tcatgctgtt actcgttaca tcgttaacga agtacaggac gtataccgtc tgcagggcgt
    12661 taagattaac gataaacaca tcgaagttat cgttcgtcag atgctgcgta aagctaccat
    12721 cgttaacgcg ggcagctccg acttcctgga aggcgaacag gttgaatact ctcgcgtcaa
    12781 gatcgcaaac cgcgaactgg aagcgaacgg caaagtgggt gcaacttact cccgcgatct
    12841 gctgggtatc accaaagcgt ctctggcaac cgagtccttc atctccgcgg catcgttcca
    12901 ggagaccact cgcgtgctga ccgaagcagc cgttgcgggc aaacgcgacg aactgcgcgg
    12961 cctgaaagag aacgttatcg tgggtcgtct gatcccggca ggtaccggtt acgcgtacca
    13021 ccaggatcgt atgcgtcgcc gtgctgcggg tgaagctccg gctgcaccgc aggtgactgc
    13081 agaagacgca tctgccagcc tggcagaact gctgaacgca ggtctgggcg gttctgataa
    13141 cgagtaatcg ttaatccgca aataacgtaa aaacccgctt cggcgggttt ttttatgggg
    13201 ggagtttagg gaaagagcat ttgtcagaat atttaaggaa tttctgaata ctcataatca
    13261 atgtagagat gactaatatc ctgaaactga ctgaactaat tgagtcaaac tcggcaagga
    13321 ttcgatacta ttcctgtgta actttcttaa ggaacgagaa tgaaacagga agtggaaaag
    13381 tggcgacctt ttggacatcc ggatggtgat attcgtgatt tatcatttct tgatgctcat
    13441 caggctgtct acgttcagca tcatgagggc aaagagcctt tagagtatcg cttttgggtt
    13501 acctactctc ttcactgctt cacaaaagat tatgaacatc agacgaacga agaaaaacaa
    13561 tcgttaatgt accacgcgcc taaagaatct cgtcccttct gccagcaccg ttataactta
    13621 gcgcacacac ttaaaaagaa ctattttggc gctgccagaa agcaacgtta ttcatgccgg
    13681 gtatggtagc tatgccgtga ttgaggtgga cttagacgga ggagataagg cattttactt
    13741 tgttgcgttc agggctttca gggaaaagaa aaaactccgt ttgcatgtaa ctagcgctta
    13801 tcccatttct gaaaaacaga aaggtaaatc agtgaaattt ttcaccattg cctacaactt
    13861 attgagaaat aagcagcttc ctcagccctc aaaataacaa aacccacctt aaggtgggtt
    13921 tcgccagaga attatctctg gtattcagaa cgccattacc ggactttgcc ttgaccttgc
    13981 gataatcgca ggttgcggga tgtctgaatt tcttcagtct gctgcatcct ggaagatgag
    14041 aacatgtgtt cttattttcg tctctatcat agttgagtat ttactctctt acaatcagat
    14101 ctctttcatt gctcaacagg cgatggcttc agactttgca ttacggaatt tttaagaaag
    14161 gcagggcgaa acgaggaaga agcttttcgc cctggacaac attcctgctg acggcactac
    14221 cataaaaaaa cacaacgctt tcaacaacct tgcgtttttc atcttccgta gcggctcgga
    14281 ataaatttac gttcttacat tccgtctcat agtctttgcg aggcccgtcc cagatagctg
    14341 tcccagtctt tccataccgg ctgcaaaccc tgagcggtta acgcggcagc aaccgcttcc
    14401 ggtctgcgat cgtcgtgcgg tgagaactgt tccaactcgg ggtgattatc ggcatagcca
    14461 cctggctgcg ttttcgagaa ggcgctgacg ttattgatcg ccagcggaat aacgcgatcg
    14521 cgaaaccacg gtgattcccg cgtggagagt gacagttcaa tctccggtgc aagcagtcgg
    14581 aaggcgcaga tggtttgcac taactggcgt tcatccataa tcgacgcagg ctcaatgccg
    14641 ccagtacacg ggcgcaggcg cggaaaggag acagagtaac ggctttgcca gtaatgctgt
    14701 tgcagccata gcaaatgttc ggcaaccata tagcagtcaa cgcgccagtt gtcggaaagg
    14761 ccaattagcg cgccgaggcc tatcttatca atccccgcac gccccagccg atccggcgtt
    14821 tccagccgcc agaagaagtc ctgttttttt cctttcagat gatggcgggc ataagtcgcc
    14881 tcgtgatatg tctcctgata aaccatcacg ccatccagac caagctgctt taactcggcg
    14941 tattccgtct ccgccagcgg ttgcacttcc atttgtagtg aagagaactg ttcacgcagg
    15001 gcggggagat gacgacgaaa gtaatccatc cccactttcg cctgatgttc accagtgact
    15061 aatagcagat gttcaaagcc catctcccgt atagcggcgc tttccctggc aatatccgct
    15121 tcatccagcg ttttgcgctt gatgcgatta ctcatggaaa atccgcagta cgtgcagtcg
    15181 ttagcgcaaa gattggaaag ataaagcggg acgtagaaac taactgtgtt gccaaatcgc
    15241 tgacgggtca gacgctgcgc ccgttgggcc agttgttcca gatagccact ggcggctggc
    15301 gataacagcg ccatcatatc gtcgcgggtg agttgcgagg catttagcgc ccgttctacg
    15361 tcagcagccg ttttgccgtt gatacgcagg cggatgtcgt cccagtccag ttgtcgccag
    15421 cgatcgctga aggttttcat gccgatgcct ccagaaatcc agtcagcggg ctggtggcat
    15481 gagcaaaatg actgcggctg cccggtccgg actgacgtgc cagcaggcct gcttctaccg
    15541 ccagacgaaa tgccttcgcc atgttgacgg gatcgtccgc gacggcaatt gccgtattca
    15601 ctaacaccgc gtcggccccc atttccagcg cctgcgcggc atggctggga acgccgatgc
    15661 cagcatcgac aaccaccggc actgtggcct gctggataat aatctccagc atggcgcggg
    15721 tttccagtcc ctgattcgag ccaatcggcg cgccgagcgg catcaccgct gcacagccga
    15781 cttcttccag acgtttacac aataccggat cggccccgca gtaaggcagc acgacaaatc
    15841 cctgttgtac cagcgtttcg gcggctttca gggtttcgat gggatcgggc aacagccagc
    15901 gggcgtcagg gtgaatctct aattttaacc agtttgtgcc taacgcttca cgagccagat
    15961 gggcggcgaa aatggcttct tccgctgttt tcgccccgga tgtatttggc agcagggtca
    16021 cacccgccgc gataagcggt tcgaggatag cgtcgttgtg ctggcgcaag tcgacacgtt
    16081 tcatcgccag tgtcaccagc tggctgccgg aagcgcggat cgcctccacc atcagttgtg
    16141 aagaagcgaa tttccctgtg ccggtaaaca gatgtgaatc aaacgttttg tccgcaatac
    16201 gtaacatttc aaccccctgc aataacctga aaaagcagga tctggtcgcc atcctgcacg
    16261 atatgttgcg cccactgctc acgcgggacg atttgctgat taatcgccag agccgcgccc
    16321 gcttgtcgtt ggtcgagttg ctccagtagt tcgtgaacag tttgcccggc ggcgcactgc
    16381 atcggttgat cgttaaacag gatctgcatt gcttcctccg cataccgggc aaccactggc
    16441 gcggcgcaac gccaggctgc gccactggct cgatttaccg tcgaacagtc ggagttctcc
    16501 cgcaggtgtc tctataccgc ttaataactt aatggcttcc agtgcctgca aagtgcccat
    16561 aaccccgacc accgggccaa ccacgcccgc cgtgcggcag ttgcgttctg gctcctggtt
    16621 atctggccac aggcagcggt aacacccctg ctcccagggc ggcgtcagta ccatcaactg
    16681 accgccaaat ccgaccgcgc tggcggtgat aagcggcgtg ttgagtgcca cgcaggcggc
    16741 attaatctcc tggcgagtcg ccatattgtc ggtacagtcg agcaccacat cggcccgtgc
    16801 aaccgcatct tttaacgcct cacccgttaa ccgttgttgt aatgctgtca gttgaatgtc
    16861 gggattcaac tgtgtcagtc gctgttggct gacctgcgat ttcgggcgat cgatatcttc
    16921 agtggtaaag aggatttgtc gttgcagatt gcttaaatgc acatcgtcgt catctgccag
    16981 taccagcgtc ccgacgccag cgcccgccag gtacagcgca gcaggtgtac ccagcccgcc
    17041 cagaccgata atcagcacct ggctgtcgag cagtttttgc tgcccgtcca gagcgatatc
    17101 gtcgagcagg atttggcggc tataacgcat aaagtcacgg tcattcatcg ccaactcctg
    17161 caatttccag caactgtgcc gttgccaaac gccagtctgc ggcttgagta atggcgctga
    17221 cgacggcgat actgccgaca cccgttgcta tcaccgcagg cgcgcgtgcc agactgatac
    17281 cgccaatcgc cacggtggga taatccgcca gtcgctcaac atgccgtgcc agctgttcca
    17341 gcccctgcgg tgcagaaggc atctgtttgg tttgcgtcgg gaacacatgt cccagcgcga
    17401 tataagaggg gcgtgctgcc agcgcgacgt cgatttccat atcgtcatgt gtcgaaacgc
    17461 ccagccgcag gcctgccgcg cggatggcat tgagatcggt ggcttgcaaa tcttcctgcc
    17521 ccaaatggac gccatacgcc tgatgcttga tcgccagccg ccagtaatcg ttgataaaca
    17581 atcgcgcgtt atagcggcgg cccagcgcaa ttgccgccac gacatcggct tccacctctt
    17641 catcgcgccg atctttgatg cgtagctgga gagtacgtac gcctgcatcc aacagacgtt
    17701 cgatccactg tacgctgtcc accaccgggt acagtcctaa acgaaaaggt acaggaggaa
    17761 aatcaggctg atacatcacg cttcctcctt acgcaggtag atttctccgc ctctggcacg
    17821 gaagttctcc gacatatccg ccattcccat ttcaatagtt tgcgtggcgg cgtaatcacg
    17881 cacttcctgg ctgattttca tcgagcaaaa tttcggtcca cacatggagc aaaaatgggc
    17941 gactttgccg gactcttggg gcagggtttc atcgtgataa gcgcgggcgg taaacgggtc
    18001 gagggccaga ttaaactggt cttcccagcg aaattcgaag cgggctttcg acatggcgtt
    18061 atcgcgaatt tgcgcgcccg gatgcccttt cgccaggtca gcggcgtggg cggcaatctt
    18121 ataggtgata agcccctgct taacatcttc tttattaggc agacccagat gctcttttgg
    18181 cgttacgtaa cagagcatcg cgcagccaaa ccagccaatc atcgccgcac caatccccga
    18241 cgtgaagtgg tcatagcccg gcgcaatatc ggtagttagc ggccccagag tgtaaaacgg
    18301 cgcttcgtgg cagtgctcta actcctcggt catattgcgg cggatcatct gcatcggcac
    18361 gtggcctggg ccttcaatca tcacctgcac gtcatattcc caggcaattt tggtcagttc
    18421 gcccagcgta tgcagctcgg caaactgcgc ttcatcgttg gcgtcctgaa tagaaccggg
    18481 gcgcagaccg tcgcccagcg acagcgaaac gtcataagcg gcacagattt cacaaatttc
    18541 gcggaagtgt tgatagagga aattttcctg atgatgggag aggcaccatt tcgccataat
    18601 cgaaccgccg cgagagacga taccggtcag gcgtttcgcg gtcatcggca catagcgcag
    18661 cagtacgccc gcatggatag tgaagtaatc cacaccttgc tcggcctgtt ccagcagcgt
    18721 gtcgcggaac gcttcccagg taagatcttc ggcgatcccg ttaaccttct ccagcgcctg
    18781 gtagatcggc actgtaccga tcggcaccgg gctgttacgc aaaatccact cgcgggtttc
    18841 gtgaatatag cgaccggtgg agagatccat caccgtatcc gctccccagc gcgtggacca
    18901 taccagcttt tccacttctt cttcgatgga agaggtgacc gcagagttgc cgatattggc
    18961 gttaactttt accaggaaat tgcgaccaat aatcatcggc tccgattccg gatgattaat
    19021 gttggccggg ataatcgcac gtccggcagc aacttcatca cggacaaatt ccgcagtgat
    19081 attttccggc agacgtgcgc caaagctcat tcccggatgc tggtggcgta aaacttcgct
    19141 acggatgcgc tcgcggccca tattctcgcg gatggcgatg aattccattt ccggtgtgat
    19201 gatgccctgg cgggcgtagt gcagttgggt gacgcggcgt cctgctttgg cgcgttttgg
    19261 tgttagtacg ccgctaaaac gcagttcgtc gaggccatca tctgccagcc gcgctttagt
    19321 gtaatcggaa ctgcgcacgg taagttcttc ggtatcgccg cgcgcatcga tccacggctg
    19381 gcgtagtttt gccagccctt gctgcacgtt aatggcaatc tgcggatcac catacgggcc
    19441 ggaggtgtcg tagaccggaa tcgcttcgtt ttcttcgtac tgcggctgtt ctttgctacc
    19501 gccaattagc gtcgggctaa gctggatctc acgcatcggc acgcgcacgc cggggtgtgt
    19561 gccagtgata taaatgcgtt ttgagttggg aaaggcggtg ccttccaggg tgtcgataaa
    19621 atgttgggcc cgggcgcgtt gttcgcggcg ggtcagtttt gttgcagaca tagctcattc
    19681 caaaaagtta aggacgtggc ttgtcagacg acggatgaag caagagacga tcgccgcagg
    19741 ggcgatgcga tagcagatta actcttgttc ccttcgcagg tattagcctg atcaggttcc
    19801 gcggatcccg aataaacggt ctcagccagt taaggcactc cgacaagaaa ttagccccgc
    19861 aaatggggca ttgaatgtaa attacgcgtt aacagcgcag aactcaagca ggatgtttga
    19921 cgcgggcggc gtcaagcacc agcagaatca gcttatcttc cagcacaaag cgcgcttcca
    19981 gcgcctcgcc gatgtcagat aaaacctgtt gaaactcaag gtaattatca tgatcgatgg
    20041 cggtttccag actggaatcg tagtaatcca taatctgttg agtattggct tcgagttgcg
    20101 gccaaatctt cgcggcgcgc gcgagttgcc cgttgccttc cagcttatga agaatgcgct
    20161 cataaatact gaaatgtccg gcagacaagt aatcgaccag gctctgacaa aaatcatcaa
    20221 gggctttttc gtttagcctc atgtacgatt ctttgccagg cttaatgcca accagattgt
    20281 agtaagccac gagcagatgc ttacgtacat gtagccagcg atcaaccagt ttgttacttc
    20341 ctctgacgcg ctccgtcagg ttatcgagct ggttaagcat gattgactcc gcaagtttgt
    20401 attcaaaaac tgctcagtga gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag
    20461 gtagtgcaag agctatggat cgtataattg aaaaattaga tcacggctgg tgggtcgtca
    20521 gccatgaaca aaaattatgg ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt
    20581 tcgatcttgt gggtcagcgc gcactacaaa tcggcgaatg gcagggggaa cctgtttggt
    20641 tagtacaaca gcagcggcgt cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg
    20701 ttgggctgtt tcaactggcc ggacgaggcg tacaactggc ggagttttac cgatcgcata
    20761 aatactgtgg ttactgcggg catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt
    20821 gcagccattg ccgtgagcgt tactacccgc aaatcgcccc ctgcattatt gttgctatcc
    20881 gtcgcgatga ttcgatcctc ctcgcccagc atacccgcca tcgtaacggt gtccatacag
    20941 tacttgccgg attcgtcgaa gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga
    21001 tggaagagag cggaattaaa gttaaaaact tgcgttacgt gacttctcag ccgtggccgt
    21061 ttcctcagtc tttaatgacc gcgtttatgg cggaatatga cagcggcgac atcgtgatcg
    21121 acccgaaaga attgctcgag gcgaactggt atcgctatga cgatttgccg ttactcccgc
    21181 cgcccggcac cgtagcgcgc cgtctgatag aagatacggt ggcgatgtgt cgggcagagt
    21241 atgagtgatg atacactgac cgcctgacgc actaaggaac agccaaaatg accgaactta
    21301 aaaacgatcg ttatctgcgg gcgctgctgc gccagcccgt tgatgtcact ccagtatgga
    21361 tgatgcgcca ggcgggtcgc tatctaccgg aatataaagc cacgcgcgcc caggcaggcg
    21421 attttatgtc gctgtgcaaa aacgccgagc tggcgtgcga agtgactttg caaccgctgc
    21481 gtcgctaccc gctggatgcg gcgatcctct tttccgatat cctcaccgtg ccggacgcga
    21541 tggggttagg gctctatttt gaagccggag aaggtccgcg ttttacctcg ccagtcacct
    21601 gcaaagctga cgtcgataaa ctgccaattc cggacccgga agatgaactg ggttacgtga
    21661 tgaacgcggt gcgtaccatt cgtcgcgaac tgaaaggcga agtgtcgctg attggttttt
    21721 ccggcagccc gtggacgctg gcgacctaca tggtggaagg cggcagcagc aaagccttca
    21781 ccgtgatcaa aaaaatgatg tatgccgatc cgcaggcgct gcacgctctg ctcgataaac
    21841 tggcgaaaag cgtcactttg tatctgaatg cgcagattaa agccggtgct caggctgtga
    21901 tgattttcga cacctggggc ggcgtgctta ccgggcgcga ttatcaacag ttctcgctct
    21961 attacatgca taaaattgtt gatggtttac tgcgtgaaaa cgacggtcgc cgcgtgccag
    22021 tcacgctgtt taccaaaggc ggcggacagt ggctggaagc gatggcagaa accggttgcg
    22081 atgcgctggg cctcgactgg acaacggata tcgccgatgc gcgccgccgc gtgggcaata
    22141 aagtcgcgtt gcagggcaat atggatccgt cgatgctgta cgcgccgcca gcccgcattg
    22201 aagaagaagt agcgactata cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc
    22261 ttggtcacgg cattcatcag gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag
    22321 tgcatcgact gtctgaacag tatcaccgct aaggagtgat tatggatctc gcgtcattac
    22381 gcgctcaaca aatcgaactg gcttcttctg tgatccgcga ggatcgactc gataaagatc
    22441 caccggatct gatcgccgga gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag
    22501 cggcgatggt gctgctgaaa tatccctcgc ttgagctggt ggagtataaa gtggcccgca
    22561 tcgccaccac catgccttac attccaggtt ttctttcctt ccgcgaatat cctgcgctgc
    22621 tggcagcgtg ggagatgctg tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga
    22681 tctcgcatcc ccgccgtctt ggcgtcgcca gccattttgg cttattggtg gatgtgccga
    22741 ccattggcgt ggcgaaaaaa cggctctgcg gtaaattcga accgctctcc agcgaaccgg
    22801 gcgcgctggc cccactgatg gataaaggcg agcagctggc ctgggtctgg cgcagcaaag
    22861 cgcgctgtaa cccgttgttt atcgctaccg gccatcgggt cagcgtggac agcgcgctgg
    22921 cgtgggtaca acgctgcatg aaaggctatc gtctgccgga gccaacgcgc tgggcggacg
    22981 cggtggcctc ggaacgtccg gcgttcgtgc gctatacagc aaatcagccc taattcaggt
    23041 aaactgcgga taatttccgt atttgagaac tcaacatgtt acaaaaccca attcatctgc
    23101 gtctggagcg cctggaaagc tggcagcatg tcactttcat ggcttgctta tgcgaacgca
    23161 tgtaccccaa ttacgccatg ttctgccagc aaaccggttt tggtgatggg caaatttacc
    23221 gtcgcattct cgatctcatc tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg
    23281 acagccaact ggagaaattt gaagaagcga ttccttcagc cgacgatttc gatctgtacg
    23341 gcgtttatcc ggcaatcgat gcctgcgtgg cgttaagtga actggtccat tcgcgtttga
    23401 gtggtgaaac gctcgaacac gcggtggaag tgagtaagac ctccatcacg accgttgcga
    23461 tgctggaaat gactcaggct ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag
    23521 ctgtagagca agaatgggac attcagtggg aaatattccg acttttagct gagtgcgaag
    23581 aacgcgatat cgagctgata aaaggcctta gggcagacct gcgtgaggcg ggtgatagca
    23641 atattggtat aatttttcag caataagacc agaaaacgtg atttaacgcc tgatttgtcg
    23701 tacctggagt cttccctttc gccccccgtc tggtctacat ttggggggcg aaaaaaagtg
    23761 gctatcggtg cgtgtatgca ggagagtgct attctggcat ttccgtcgca ctcgatgctt
    23821 agcaagcgat aaacacattg taaggataac ttatgaacaa gactcaactg attgatgtaa
    23881 ttgcagagaa agcagaactg tccaaaaccc aggctaaagc tgctctggag tccactctgg
    23941 ctgcaattac tgagtctctg aaagaaggcg atgctgtaca actggttggt ttcggtacct
    24001 tcaaagtgaa ccaccgcgct gagcgtactg gccgcaaccc gcagaccggt aaagaaatca
    24061 aaattgccgc agctaacgta ccggcatttg tttctggcaa ggcactgaaa gacgcagtta
    24121 agtaagattg cgtggcagtg aacagtttta acgaaggggt ggtttcaccc cttttgtctt
    24181 tctggcgtcg atcattgatg ctggcaggtg cgctgtttct cactgcctgt agccacaact
    24241 cttcacttcc tccctttacc gccagtggat ttgctgaaga ccagggcgcg gtacgcatct
    24301 ggcgaaaaga cagcggcgat aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg
    24361 gcgataccac gacgcgagag tatcgctggc agggcgataa cctcacgctc atcaatatca
    24421 atgtttacag caaaccgccg gtgaacattc gcgcgcgttt tgacgatcgc ggtgatctga
    24481 gctttatgca acgtgaatcc gatggggaaa agcagcagct ttctaacgac caaatcgatt
    24541 tataccgtta tcgtgctgct cagatccgcc agattagcga tgccttacgt caggggagag
    24601 tcgtgctgcg ccaggggcgc tggcatgcga tggaacagac cgtgaccacc tgcgaagggc
    24661 aaaccattaa acctgattta gattcgcagg cgatagcgca tatcgagcgc cgccagagcc
    24721 gctcttctgt tgatgtcagc gtggcatggc tggaagcgcc cgaaggttcg caattactgt
    24781 tagtggcaaa ctctgatttc tgtcgctggc aacccaacga gaaaacgttc tgatttacca
    24841 gtggcccata cccatatgac cgccaccacc gcagccgccg tagcccatgc ccattccggc
    24901 tccgcgcgga atacccgctt cagccatcgc gatatcccgt ttcacccgta actcatctaa
    24961 cgactgacgc aaattctcca tctctttggc gaccgcgtta attttgctgc tatccggtgg
    25021 gttcgcggct aacagggcat tgtattcata acgcttcgtc accagttgct gttgcagtgc
    25081 gctgctttga gcgtaaaagt cattatggat tttctgccac gctgtctgtt gttcgctggt
    25141 caaaggcgcg gcattttgct gccacatacc gtgtccgccg tgagcaaatg cagatgtcga
    25201 tcccatcgcc attgctgaaa gcgccatcat taccagggca attttcgtgt tccgtttcat
    25261 ggttaatcct ccagtggttg tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa
    25321 cgctgatatg acgggtaatc tggcatgata aacgagtaaa aatgactcgc ctgctgcggg
    25381 tagcgagtca tttttactca ttgaaactta agcctttgtg ttacagcgca gggtaagcgc
    25441 tgataaaaga tggcatgatt tctgctgtca gaaagggatg agcaggcaaa gaagaagatg
    25501 cgttttatgc aacgttctaa agactcctta gctaaatggt taagcgcgat cctccccgtg
    25561 gtcattgttg ggctggtagg gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag
    25621 actgccgccg ccagacaaac gctgctggaa aaaggcagtg tacttattcg cgctcttgaa
    25681 tccggctcgc gcgtcggcat ggggatgcgc atgcatcatg cgcagcagca ggccttactg
    25741 gaagaaatgg ccgggcagcc tggagtacgt tggtttgcgg tcacggatga acaaggaaca
    25801 atcgtgatgc atagcaactc cggcatggtg ggaaaacagc tttattcccc gcaggaaatg
    25861 cagcagttac atccgggaga tgaagaagcg tggcggcgga tcgatagcgc agacggcgag
    25921 cctgttctgg aaatttatcg ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg
    25981 cgccatatgc agcagtatgc cgcgacacca caagcaattt tcatcgcttt cgacgccagt
    26041 aatattgtga gtgccgaaga tcgtgagcag agaaacaccc tgattatcct cttcgccctg
    26101 gcgacggtct tgctggcaag cgtattgtca ttcttctggt atcgccgcta tctgcgctcg
    26161 cgccagcttc tacaagatga aatgaagcgc aaagagaagc tagtggcgct ggggcatctt
    26221 gcggcaggcg ttgcccacga aatccgtaac ccactttcct cgattaaagg actggcgaaa
    26281 tactttgccg agcgcgcgcc tgcaggggga gaagcgcatc aactggcgca ggtgatggcg
    26341 aaagaggccg accgtttaaa ccgcgtggta agcgagctgc tggaactggt taagccaacg
    26401 catctggctt tgcaggcggt ggatctcaac acgctgatta accactcatt acagctggta
    26461 ggccaggatg caaacagccg ggagatccag ttacgcttta ccgccaacga cacattaccg
    26521 gaaattcagg ccgatccgga caggctgact caggtcctgt tgaatctcta tctcaatgct
    26581 attcaggcga ttggtcagca tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc
    26641 gtgaaaatca gcgttaccga cagcggtaag ggaattgcgg cagatcagct tgaagccatc
    26701 ttcactccgt acttcaccac caaagccgaa ggcaccggat tggggctggc ggtcgtgcat
    26761 aatattgttg aacaacacgg tggtacaatt caggtcgcaa gccaggaggg aaaaggctca
    26821 acgttcaccc tctggcttcc ggtcaatatt acgcgtaagg acccacaagg atgacgcacg
    26881 ataatatcga tattctggtg gtggatgatg acattagcca ctgcactatt ttgcaggctt
    26941 tactgcgcgg ctggggctat aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc
    27001 aggtgcggga gcgggttttt gatcttgtgc tttgcgatgt gcgaatggca gagatggacg
    27061 gcattgccac gctaaaagag atcaaagcgt taaatccggc gattccggta ctgattatga
    27121 ccgcatactc cagcgtcgag acggcggtag aggcactgaa aaccggggcg ctggattatc
    27181 tcatcaagcc gctggatttc gacaccctgc aggcgacgct ggaaaaggcg ctcgcgcata
    27241 cgcacagtat tgatgctgaa acgcctgcgg tgactgccag ccagttcggt atggtcggta
    27301 aaagcccggc gatgcaacac ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca
    27361 cggtgctgat ccacggcgat tccggcaccg gtaaagagct ggtcgccagg gcgattcacg
    27421 ccagtagcgc acgtagcgaa aaaccgctgg taacgctcaa ctgtgtggca ctcaacgaat
    27481 ccttgctgga atctgaattg ttcggtcacg aaaaaggggc gtttactgga gccgataaac
    27541 gccgggaagg gcgctttgtt gaggcggacg gcggcacgct gtttctcgat gaaattggcg
    27601 atatctcgcc gatgatgcag gtgcgtctgc tgcgtgcgat tcaggagcgc gaagttcagc
    27661 gtgtcggtag caaccagact atctcggttg atgtccggct gattgcggcg acccatcgcg
    27721 atcttgccgc agaggtgaat gccgggcgtt ttcgccagga tctctactat cgcctgaatg
    27781 tggtggcgat tgaagtacca tcgctgcggc aacggcggga ggatatccct ctgctggcta
    27841 gccattttct tcagcgtttt gccgagcgta atcgcaaggc ggtaaaaggc tttacgccac
    27901 aggcgatgga tttgctgatc cactacgact ggccgggaaa cattcgtgaa ctggaaaacg
    27961 cggtggaaag gtcggtggtg ctgctgaccg gggaatatat ttccgaacgc gagctgccgc
    28021 tggcgattgc cagtacgccg atcccgctgg ggcaaagtca ggatattcag ccgctggtgg
    28081 aagtcgaaaa agaagtgatt ctggcggcac tggagaaaac gggcggcaac aaaaccgaag
    28141 ccgcccgtca gttagggatc acgcgcaaaa cgctattggc aaaactgtcg cgttagttct
    28201 gctcgcgttc gatagcgcgc cagccgatat ctttccggca gaagcagtcg tcccagtgaa
    28261 tatcggtcat taaggcataa gcgcgtttct gcgcttctgc cacggtatga cccagcgcgg
    28321 tgacgcacag tacgcgcccg ccgctggtca ctacctgctc gttatccgcc agtttcgtgc
    28381 ccgcgtggaa cactttgccg cctgccactt cttccagcgg caggccgtgg atcacgtcac
    28441 cggtgcggta atcacccgga tatccacccg cagccatcac cacgccgaga gaagcgcgtt
    28501 catcccactc tgacgttttc tcgtccagtt tgccttcaca ggccgccagg cagagctcaa
    28561 ccagatcgga cttcatgcgc agcataatcg gctgggtttc tggatcgcca aagcggcagt
    28621 taaattcgat aaccttcgga ttgccctgtt tgtcgatcat caggcccgcg tagagaaaac
    28681 cggtgtaggt gttgccttcc gccgccatgc ctttcacggt tggccagatg atacgttcca
    28741 tggtgcgctg atgaacttca tcggttacta ccggcgcggg ggagtaagcg cccatcccgc
    28801 ccgtgtttgg tccggtatct ttatcgccta cgcgtttgtg atcctggctg gtggccatcg
    28861 gcagcacatg ctcgccgtct accatcacga taaagctcgc ttcttcgcca tcgaggaact
    28921 cttcgataac gatgcgatga cccgcgtcgc caaaagcgtt gcccgccagc atatcgtgaa
    28981 cagccgcttc cgcttcttcc agcgtcatcg ccacgataac gcctttcccg gcagccagac
    29041 cgtccgcttt aatgacgatt ggcgcgcctt tctcacgcag atacgccagc gcaggttcta
    29101 cctcggtgaa gttctggtat tccgccgtag ggatcttatg gcgggccagg aaatctttgg
    29161 taaacacttt tgagccttcc agttgggccg cacctgcggt tgggccgaag attttcagcc
    29221 cggcggcgcg gaaggtatcg accacgcctt tcaccagcgg cgcttccggg ccgacgatgg
    29281 tcagatcaat cttttcgttt tgtgcgaaat ccaacagcgc cgggatatcg gtcacgccaa
    29341 tagcaacgtt ttgcagcgcg ggttccagtg cagtgcctgc attacccgga gcaacaaaaa
    29401 cagtctcaac cagcggcgac tgggccgctt tccaggccag cgcgtgctcg cgcccgccgt
    29461 taccaatcac taatactttc atctattgct ccattaatgg cggaagtggc gcatgtcggt
    29521 gaagagcatc gcaataccgt gctcgtcggc ggcggcaatc acttcgtcat cacggatgga
    29581 accgccaggc tggattacgc aggtcacgcc cgcagcggcg gcggcatcaa taccgtcgcg
    29641 gaacgggaag aacgcgtcag aagccatcga ggaacctttc acttccaggc cttcatcggc
    29701 agctttaata ccggcgattt tcgcggagta cacgcggctc atctggcccg cgccaatacc
    29761 gatggtcata ttgtttttgg catagacgat agcattggat ttcacgaact tcgccacttt
    29821 ccagcagaac agcgcatcgc gcagttcctg ttcggtcggc tgacgttggg tgacgacgcg
    29881 cagttcttcc gcaccgacca tccccaggtc acgatcctga accagcagac cgccgttcac
    29941 gcgtttgaaa tcaagacccg gaacacgctc gccccactga ccgcaggtca gaacgcgtac
    30001 gttctgcttg gcggcggtga ttttcagggc ttcttcgctg gcggaaggcg caataatcac
    30061 ttcgacaaac tgacgagaaa tgatggcctg tgcggtttcc gcatctagct cgcggttaaa
    30121 ggcaatgatg ccgccgaatg cggaggttgg gtcggttttg tacgcgcgat cgtaagcatc
    30181 aagaatggaa ttgccgatag ctacaccgca agggttggcg tgcttcacaa tcacacatgc
    30241 cggctcggcg aactctttca cgcactccag cgccgcatcg gtatcggcga tgttgttata
    30301 agagagggct ttaccctgaa cctgggttgc ggtagcaacg gaggcttctt tcacattctc
    30361 ttctatatag aaggcagcct gctggtggct gttctcgccg taacgcatat cctgcttctt
    30421 aatgaagttc aggttcagcg tgcgtgggaa gcgaccagcg gcttctttgc tttcaccgtg
    30481 gtacgccgga accatgctgc cgaagtagtt ggcaatcatg ctgtcgtagg cggcagtgtg
    30541 ttcgaaggct ttgatggcga ggtcgaaacg ggttgcaagc gtcagcgatc cttcgttgtc
    30601 atccatctct ttaataatgg cgtcgtagtc gctgctcttc accacgattg cgacatcttt
    30661 atggttctta gcggcggagc gcaccatggt cgggccgccg atatcgatgt tctcaaccgc
    30721 atcttccagc gagcaacctt cacgggccac ggtctgggcg aacggataca ggttaacaac
    30781 caccatatcg ataggctgga tctgatgttc ttccataatg gcatcgtcct ggccgcgacg
    30841 gcccagaatg ccaccatgta ctttcggatg cagggtcttc acgcgtccat ccatcatctc
    30901 cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga cctttttctg ctaacagacg
    30961 ggcagtgccc cctgtagaca gcagctccac accgcgtgcg gaaagtgcct gggcgaattc
    31021 gacgataccg gctttgtcag aaacactgag cagagcgcgg cggactggac gacgttgttg
    31081 catggtaaat cccctggatt tgactattac agagagcgtt agctgaattt ttcgcgaaaa
    31141 actcagctaa cgcccctaac ggggcatcct tatttttcgc ccgcattgta acgaaaacgt
    31201 ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc acaattttga cagtggttac
    31261 cgtgggaaaa atacagaaat tacattgatg attgtggata actctgtgcg taaaaaggta
    31321 taaagcgggg ttttgctggg gaatgcagca gtcagtcatt tttctgcaat ttttctattg
    31381 cggcctgcgg agaactccct ataatgcgcc tccatcgaca cggcggatgt gaatcacttc
    31441 acacaaacag ccggttcggt tgaagagaaa aatcctgaaa ttcagggttg actctgaaag
    31501 aggaaagcgt aatatacgcc acctcgcgac agtgcgctaa a
//
LOCUS       DAZGTR010000047        30859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGTR010000047 DAZGTR010000000
VERSION     DAZGTR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.047"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..377
                     /gene="ybiJ"
                     /locus_tag="ACHLCY_002340"
     CDS             117..377
                     /gene="ybiJ"
                     /locus_tag="ACHLCY_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP0369587.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(481..540)
                     /gene="ybiE"
                     /locus_tag="ACHLCY_002341"
     CDS             complement(481..540)
                     /gene="ybiE"
                     /locus_tag="ACHLCY_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP0369588.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(607..1692)
                     /gene="hcxB"
                     /locus_tag="ACHLCY_002342"
     CDS             complement(607..1692)
                     /gene="hcxB"
                     /locus_tag="ACHLCY_002342"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP0369589.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHKHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(1833..2795)
                     /gene="ybiB"
                     /locus_tag="ACHLCY_002343"
     CDS             complement(1833..2795)
                     /gene="ybiB"
                     /locus_tag="ACHLCY_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP0369590.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(2823..4973)
                     /gene="dinG"
                     /locus_tag="ACHLCY_002344"
     CDS             complement(2823..4973)
                     /gene="dinG"
                     /locus_tag="ACHLCY_002344"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP0369591.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTSIAIDDDLWRRLSTDKASCLNRNCYYYRECTFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            5093..5575
                     /gene="ybiA"
                     /locus_tag="ACHLCY_002345"
     CDS             5093..5575
                     /gene="ybiA"
                     /locus_tag="ACHLCY_002345"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFP0369592.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(5808..7172)
                     /gene="rhlE"
                     /locus_tag="ACHLCY_002346"
     CDS             complement(5808..7172)
                     /gene="rhlE"
                     /locus_tag="ACHLCY_002346"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP0369593.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDICVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            7401..8072
                     /gene="cecR"
                     /locus_tag="ACHLCY_002347"
     CDS             7401..8072
                     /gene="cecR"
                     /locus_tag="ACHLCY_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP0369594.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            8075..9070
                     /gene="hlyD"
                     /locus_tag="ACHLCY_002348"
     CDS             8075..9070
                     /gene="hlyD"
                     /locus_tag="ACHLCY_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP0369595.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            9063..10799
                     /gene="ybhF"
                     /locus_tag="ACHLCY_002349"
     CDS             9063..10799
                     /gene="ybhF"
                     /locus_tag="ACHLCY_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0369596.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            10792..11925
                     /gene="ybhS"
                     /locus_tag="ACHLCY_002350"
     CDS             10792..11925
                     /gene="ybhS"
                     /locus_tag="ACHLCY_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0369597.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            11936..13042
                     /gene="ybhR"
                     /locus_tag="ACHLCY_002351"
     CDS             11936..13042
                     /gene="ybhR"
                     /locus_tag="ACHLCY_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0369598.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(13004..13414)
                     /gene="ybhQ"
                     /locus_tag="ACHLCY_002352"
     CDS             complement(13004..13414)
                     /gene="ybhQ"
                     /locus_tag="ACHLCY_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP0369599.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            13547..14308
                     /gene="ybhP"
                     /locus_tag="ACHLCY_002353"
     CDS             13547..14308
                     /gene="ybhP"
                     /locus_tag="ACHLCY_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0369600.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            14305..15546
                     /gene="clsB"
                     /locus_tag="ACHLCY_002354"
     CDS             14305..15546
                     /gene="clsB"
                     /locus_tag="ACHLCY_002354"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP0369601.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            15546..16502
                     /gene="ybhN"
                     /locus_tag="ACHLCY_002355"
     CDS             15546..16502
                     /gene="ybhN"
                     /locus_tag="ACHLCY_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP0369602.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16538..17251)
                     /gene="ybhM"
                     /locus_tag="ACHLCY_002356"
     CDS             complement(16538..17251)
                     /gene="ybhM"
                     /locus_tag="ACHLCY_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0369603.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(17456..18160)
                     /gene="ybhL"
                     /locus_tag="ACHLCY_002357"
     CDS             complement(17456..18160)
                     /gene="ybhL"
                     /locus_tag="ACHLCY_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0369604.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(18297..18749)
                     /gene="moaE"
                     /locus_tag="ACHLCY_002358"
     CDS             complement(18297..18749)
                     /gene="moaE"
                     /locus_tag="ACHLCY_002358"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP0369605.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(18751..18996)
                     /gene="moaD"
                     /locus_tag="ACHLCY_002359"
     CDS             complement(18751..18996)
                     /gene="moaD"
                     /locus_tag="ACHLCY_002359"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP0369606.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(18989..19474)
                     /gene="moaC"
                     /locus_tag="ACHLCY_002360"
     CDS             complement(18989..19474)
                     /gene="moaC"
                     /locus_tag="ACHLCY_002360"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP0369607.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(19477..19989)
                     /gene="moaB"
                     /locus_tag="ACHLCY_002361"
     CDS             complement(19477..19989)
                     /gene="moaB"
                     /locus_tag="ACHLCY_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP0369608.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(20011..21000)
                     /gene="moaA"
                     /locus_tag="ACHLCY_002362"
     CDS             complement(20011..21000)
                     /gene="moaA"
                     /locus_tag="ACHLCY_002362"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP0369609.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(20988..21132)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            21397..22305
                     /gene="yvcK"
                     /locus_tag="ACHLCY_002363"
     CDS             21397..22305
                     /gene="yvcK"
                     /locus_tag="ACHLCY_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP0369610.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(22497..24518)
                     /gene="uvrB"
                     /locus_tag="ACHLCY_002364"
     CDS             complement(22497..24518)
                     /gene="uvrB"
                     /locus_tag="ACHLCY_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP0369611.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(25097..25774)
                     /gene="bioD"
                     /locus_tag="ACHLCY_002365"
     CDS             complement(25097..25774)
                     /gene="bioD"
                     /locus_tag="ACHLCY_002365"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0369612.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(25767..26522)
                     /gene="bioC"
                     /locus_tag="ACHLCY_002366"
     CDS             complement(25767..26522)
                     /gene="bioC"
                     /locus_tag="ACHLCY_002366"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP0369613.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(26509..27663)
                     /gene="bioF"
                     /locus_tag="ACHLCY_002367"
     CDS             complement(26509..27663)
                     /gene="bioF"
                     /locus_tag="ACHLCY_002367"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP0369614.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(27660..28700)
                     /gene="bioB"
                     /locus_tag="ACHLCY_002368"
     CDS             complement(27660..28700)
                     /gene="bioB"
                     /locus_tag="ACHLCY_002368"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP0369615.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            28787..30076
                     /gene="bioA"
                     /locus_tag="ACHLCY_002369"
     CDS             28787..30076
                     /gene="bioA"
                     /locus_tag="ACHLCY_002369"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP0369616.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            30135..30611
                     /gene="ybhB"
                     /locus_tag="ACHLCY_002370"
     CDS             30135..30611
                     /gene="ybhB"
                     /locus_tag="ACHLCY_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP0369617.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
BASE COUNT         7189 a         8333 c         8019 g         7318 t
ORIGIN      
        1 ttatttgaac aaggcggtca attctcttcg attttatctc tcgtaaaaaa acgtgatact
       61 catcacatcg acgaaacaac gtcacttata caaaaatcac ctgcgagaga ttaattatga
      121 aaactatcaa tactgttgtt gctgctatgg ctctttcaac tctgtcattt ggcgtgtttg
      181 ccgcggaacc ggtaacggca tcccaggcac agaacatgaa taaaatcggc gtggtttctg
      241 ccgatggcgc atccaccctc gatgccctgg aagcgaaact ggctgagaaa gccgcagcag
      301 ccggtgccag tggatacagc atcacttccg ccaccaacaa caataaatta agcggtactg
      361 cggtaattta taagtaattc gtaggacgga taaggcgttt acgccgcatc cggcaacaga
      421 tgcctgatgc gacgctaccg cgtcttatca gtatctacaa aatccgaacc gttggacgga
      481 tcaggcgttt acgctacgtc cagcacaagt atttccctgt ctgtttgccg acagacgcat
      541 atgctctaac cctcattgat cctatgttac ccttgtttgc ccgtccgcca ctggacgggc
      601 ttttttttag ctggctaact gctgacagaa agcctgcaac gtctcttccg gcataccaat
      661 ctgccgcgct gcatcacaaa tggcctgcca gcttcccgca tccagtggaa tcccctgctt
      721 ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg gcttatcatc
      781 atcatgcggc gaggctttca cccactcggc aaaggcttcg gtctgcgcgt tacaatccgg
      841 cgcgccgaag agttccgggt tgatgatgat agtggtcatg cagttaagaa tggcatcggg
      901 actggtttgt aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc cgccaagaat
      961 ttcacacatc gctgcaaggg cgtagccttt atgttcggca aaggtcaaca gcgcccccaa
     1021 cggcgactcc tgcattaccg ccggattggt cgtcggcacg ccgttaacgt caatcaggca
     1081 acctggcggc acggggacgc ctttatgcca ggcgacgcga gttttaccaa atgcaatggc
     1141 gctggtggcg taatcaagca acagcgggaa attatcttta cgagggaaaa ccacacagaa
     1201 cggattggtg ccaaagcggc tgtcgcgacc gtggaacggc gcgaccattg gaataccgac
     1261 cacgctaaca aagtggatag agacaaaccc cgccgctgca cactgctccg cccagtaacc
     1321 gatacggccg atatgatgcg agttatgtag cgccacggcg gcaataccgt gcttatgcgc
     1381 tttctcaatc cccagcgcca tcgcttcatg tgccgcgacc tgaccaaatg cgcgatcgcc
     1441 atcgagcgtg accgccgccc ccgactcttt aacggttttg gcatgatggt taatttgcag
     1501 gtgcccctga ctccaggagc gtacatagct tgggatcatg ccaataccat gtgaatcatg
     1561 ccctgccagg tttgccgcga ttaaatgatc ggcaactaat ttcgcttctt gttcctcgct
     1621 acccatctga cgaaatacag cctgaataaa actgtgcagc gtctgagcat caaagcgatg
     1681 accactttcc atggttaact cctgtttatg ttatgtgttt gttgtatttt tatgctgcac
     1741 gccgggcgtg ggaaggtcaa taaagatgca ggaatttcat attgcggtgg ggtaaaagcc
     1801 ggatgacatg gctcatccgg ctgagaaaag aattaaaatg cctgattaac gcgcgccagg
     1861 ccgtcgctga tagttgccgc ttcacccgta gccaccaggc agcaagccat ctggattttc
     1921 agcgattcgg gaatcggttc gctgccagca aggcaacgct caatccactg cgccgtggtt
     1981 tccggatctt ttgcttgtgg cagtaactcg ctaccagcag tgtcctgttt ttcatacagc
     2041 acccgcatcc cttcacggtc aatgagattg atctgcgggc agcgctgcgg attagcatac
     2101 acttcacctt cagtaccatg cattagtagc gcccgcccgc cgatatcgct aaagaacttc
     2161 gcgacgcgtc caatgtattc cggatgcgaa acgctggaaa gacgcagcgc ctcaccttcg
     2221 gcaaatggcg ttgccagttt cgccagggta tgtgcactgt tacgcacgcc catccgccag
     2281 cgcatcgcca gttgtttttc cagcggcggg caaaacgcgc cgactggcat aaacaccggt
     2341 tgatgttcgt cgagcttcgc ctgcgcctgt ccgccgtgca acgttggcgt aatacccatc
     2401 aattcaaaaa tggtttcagt cagcacgcgg gttggatctt cgctaacccc gtgaaccacc
     2461 acaggaaaac cgagtttgtg caggagaatc gccaacaacg gcgtcaggtt ggcctgttta
     2521 cgcgcgccgt tgtaactggg gatgacaatc ggcatcggct tgcctgcagg cggcgtcagc
     2581 ttgatggtgt gattttgcat ggcttcgtaa aagccgagca tctctgcttc cccttcccct
     2641 ttgatacgca gcgcaatcaa tacgccgccc aactcaaggt cagggacttc accattgagc
     2701 atatgggcat acagaccgcg cgcagtatcc cggtctaaat cgcgcgcgtg gtttttcccg
     2761 cgcccgatct ctttaatgat tttgcgatag tccatttacg actccttacc tgactcacat
     2821 cattaacgcc gccgacggcg tggggatttc gttttttctt tctttttaac tataccttca
     2881 gggacttccg gttgctctat cggaaatacc ggtaatgcat ccagtagtcg cttgccataa
     2941 tttttggtca gcaagcgttt gtcgtagata accacttcgc cccagcaacc gtggcttcga
     3001 atcagtcgcc caacctgctg aatcaggtta aacgaggcgc tcggcaggct ttgcacctca
     3061 aacggatagc ggttgaggct tttcagccat tccccttcgg tgatcaccac cggactgtcg
     3121 atgggcggaa aagcgatttt gtggatatgc acctggctga gcagatcacc tttcaaatca
     3181 agcccttcgg caaatgactg taagcccacc agcacactac gctcaccgtt ggcaacgcgt
     3241 ttgcggtgca gttcaactaa gcgataacgc ggctgatcgc cctgcaccag cagcatcagg
     3301 cgtaaatccg tcacatagtc gagaaagcgc tgcatcgccc gtccgctggc aaacagtacc
     3361 aacataccga gatgtttttt gctctccacc tgcttacgga aaaaggccgc catttcggca
     3421 atatgctgct cttcgttgtc gatggaaggc tcaaagcgca tccggggaat aacaattttg
     3481 ccctgttcgc agtggttaaa gggggaatcc agcgccacaa aacggtcgcc cgctttctct
     3541 ttcagaccac tcatctcctg caaacgcgaa aaactgttca gcgaacgcaa ggttgcggag
     3601 gtgacaataa tgtgcggaat actgcgccac agcagccttt ccagctgatc gctgacacgt
     3661 attcccacgc agtgaaacca gagatgcagc tgcccttcgc gctcttcccg cgtcgcccat
     3721 ttggtcaccg gcgcaccgga agattgcgcc agagacgcca gccgccagag tttgctttgc
     3781 gcctcgaaca tccccaacgc gcggttcatc tgcaaaatca accgatgcag acgcacaata
     3841 tcgtggctgc cggttttctc acttaaatcg ttaagaaata actccgccag accacgcagc
     3901 atctcggtga gttttgccag ccgctggcag atctccagca cttcatcagg cagttcgccc
     3961 atcgcaaaac ggtgctctgc ctcctgcccg gcaggcatgt agagattgag aatgttgttt
     4021 aacgaggcga taagctcata caactcttca caatgcgcat tcaaacgttc agggatcgcc
     4081 agcggtggga tggtcttcgg gcgaaactgc tccatgcagg tagcgaccag tttcgtgaac
     4141 aagtccagct gtagtcgata ccagggcgcg gtgatttcgg cactcatctc cagcgcatcc
     4201 cgggccacat ctggcaggtg atgaccttcg tccagcacca gcagcagatt tttcgggtca
     4261 ggtagtacgg cttcgctttc catcgccgcc atcaccagcg catggtttgc caccaccact
     4321 tccgcttcct gaatctcccg acgagcaaca aaaaacgtgc attcacgata gtagtaacag
     4381 ttgcggttga ggcagctggc tttgtcggta cttaaacgac gccagagatc gtcatctata
     4441 gcgatagatg tatgatcgcg cagaccatcc catttataag tgtcgagatc gcccttcagc
     4501 ttcgcacaac gtttttgctc ttcctgatta tttggcgtca gttcgtcgtc aagaaacgcc
     4561 agcagatcct gttgcgtggg ctcagtgctg gcgagcgccg tcagattacg cgggcaaacg
     4621 tagcgcccac gcccaaaagc ggcagtgaat ttaagatcgg gaatgatctt tttcagcagc
     4681 ggtaaatctt tgctgtaaat ctgatcctgc aatgccacgt tggcggtact caccaccagc
     4741 gttttttgct cttcgcgggc aatggcgatg ccggggatca aataggagag cgttttccca
     4801 acgccggtgg gggcttcaat cgccagatgt cgcccttctt ctccggccag cgttttggcg
     4861 acgtccgcaa tcatctgccg ctgcggcgca cggggaataa agtcggggat ctgttcctga
     4921 agcgccttat accaggcggc aatttgcgct ttaagcgcgg cggttaatgc catgagaaaa
     4981 cctgaaatac tgtataaaca gccaatattg tggcattttt cggttttcag gggtaatggt
     5041 ttttctgttt ctggaaagcg gctcgcaata accaccaaca taatgcggtt taatgcccgt
     5101 tcgagcacaa aggatccaac acgtcatgca agacaccatc ataaatttct acagcaccag
     5161 cgatgactac ggtgattttt ctaactttgc cgcctggccc atcaaggttg acgggaaaac
     5221 ctggcccacc tcagaacact attttcaggc acaaaaattc cttgatgaaa aataccgcga
     5281 agagatccgt cgggtttctt cccctatggt cgccgcacgc atggggcgcg atcgttctaa
     5341 gcctctgcgt aaaaactggg agtcggtcaa agaacaggtg atgcgaaaag cacttcgcgc
     5401 taaattcgaa cagcatccag aactgcgtgc gctcttgctg gcaaccgcgc ccgcaaaact
     5461 ggttgagcat acggaaaacg atgcttactg gggagacggt ggtaatggta agggcaagaa
     5521 tcgactgggc taccttttaa tggagttgcg cgaacaattg gctatagaga agtaacgtct
     5581 cggtggctta tgggcggtaa aacagataaa ttttttcccg tgttctcaaa ttgctgacaa
     5641 gtgcttgttg tgaaatgccg gatgcggcgt aaacgcctta tccggcctac aaaagtatgc
     5701 aaattcaata tattgcagag ttcacgtaga tactgataag cgtagcgcat caggcagttt
     5761 tgcgtttgtt catcagcctg atgccgggca tagcccggca taaaagatta ctgcgcagcg
     5821 gtaggtttac gcggacggcg gcgacgttgt tgttcgcctg ccggtttggc atcgccgagg
     5881 cggcgagacg gtttttctgc aggtttcgcg cttgcagatt ttgcgccacc ttccccacgg
     5941 cgtggttgct gttgaccgcg accaccacct tgcccacgac cgccgccgcc acgttgctgg
     6001 cgaccgttct ggatcggttc ggctttgatt gacgggtccg gctcatagcc cggaatcgca
     6061 atgcgcggga tctctttttt cagcagtttt tcgatatcac gcagcagttt gtgttcatca
     6121 acacacacca gcgacaacgc ttcaccggta gcagccgcac gaccggtacg cccgatacgg
     6181 tggacataat cttccggtac gtttggcagt tcatagttga cgacgtgcgg caactcttcg
     6241 atatccagac cgcgcgcagc gatatcggtc gccaccagta cgcaaatgtc acccgattta
     6301 aaatcagcca gcgcacgggt acgcgcacct tgcgatttat tcccgtggat cgccgcacta
     6361 cggatgccat ctttattgag ctgctcagcc agatggttag cgccgtgttt ggtacgggta
     6421 aacaccagca cctgctgcca gttcccttta ccaatcatgt gcgacagcaa ttcgcgtttg
     6481 cgtttcttat caacaaagtg aacgtgctgg gtcacctgat cagacgcggt attgcggcgt
     6541 gccacttcga tttccagcgg gttgtgcaac agtttttccg ccagggcttt aatatcgtca
     6601 gagaaggtcg cagagaacag caggttctgg cgcttcgctg gcagttttgt taacacgcga
     6661 cggatatcgt ggataaagcc catgtcgagc atgcggtcag cttcatcgag gacgaggatt
     6721 tcaacctgat ccagcttcac tgcattttga tgttccaggt ccagcaaacg tcccggtgtt
     6781 gccaccagaa catcaacgcc gccgcgcagc ttcatcattt gcgggttaat gctgacaccg
     6841 ccaaacacca ccagcgaacg aatgttcaaa tatttgctgt aatcacggac gttttcgcca
     6901 atctgcgcag ccagttcacg ggtcggggta agaatgagcg cacgtaccgg acgacgccct
     6961 ttggcgtgcg gctggcgagt gatcaggtgt tgcaacagcg gcagcgtaaa gcccgctgtt
     7021 ttaccggtac cggtctgggc gctagccatc aggtcgcggc cttccagcac cgcagggatc
     7081 gcctgctgct gaataggggt gggttcacgg taaccctgct cggcaacggc gcgcaggata
     7141 tcagggctta aacccaaaga atcgaaagac ataactactc cgaaccgccc cgaccgttac
     7201 cggtgtagtt ttcagggaga tactgaaaag gaaaatgaca aaaaccacaa tgtcatgagg
     7261 gagcggagtg tagcagtttt tgtgacgcag cgcataaatt atccctgtga ctggcgtagc
     7321 taaaattctt agccatactg gacaactccc atatggagtc ataatcttaa tcaatcattt
     7381 gattaagaat ggactgtgcg atgaataatc ctgccatgac aatcaagggt gaacaggcga
     7441 aaaaacagct gattgctgcc gcactggcgc agtttggtga atatggaatg aacgccacca
     7501 cccgcgagat agccgcccag gccgggcaga atatcgctgc catcacctac tacttcggtt
     7561 cgaaagaaga tttgtacctc gcctgcgccc agtggattgc cgattttatt ggcgagcagt
     7621 tccgtccgca cgccgaagag gccgaacgct tgttcgcaca accacagcct gatcgcgcag
     7681 ccatccgtga actgatcctt cgcgcctgca ggaacatgat taagctgctc acccaggatg
     7741 acaccgtcaa cctcagcaag tttatctccc gtgagcagct ctctcccacg gcagcctatc
     7801 acctggtgca cgaacaggtg attagcccgc tacacagcca cctgacacgc ctgattgccg
     7861 cctggaccgg ctgcgacgcc aacgataccc gcatgatcct ccatactcat gcgttgattg
     7921 gtgagattct ggcgttccgt cttggcaaag aaacaattct gttacgtacc ggctggaccg
     7981 cgttcgatga agaaaagacc gaactgatca accagacggt gacctgtcat atcgacctga
     8041 ttttgcaagg attatcgcaa aggagtttgt agtgatgaaa aaacctgtcg tgatcggact
     8101 ggcggtagtg gtacttgccg ccgtggttgc cggaggctac tggtggtatc aaagccgcca
     8161 ggataacggc ctgacgctgt atggcaacgt ggatattcgt acggtgaatc ttagtttccg
     8221 tgttgggggg cgcgttgaat cgctggcggt ggacgaaggt gatgctataa aagcgggcca
     8281 ggtgctgggc gaactggatc acaagccgta tgagattgcc ctgatgcagg cgaaagcggg
     8341 tgtttcggtg gcacaggcgc agtatgacct gatgcttgcc gggtatcgcg atgaagaaat
     8401 cgctcaggcc gccgcagcgg tgaaacaggc gcaagccgcc tatgactatg cgcagaactt
     8461 ctataaccgc cagcaagggt tgtggaaaag ccgcaccatt tcggcaaacg acctggaaaa
     8521 tgcccgctcc tcgcgcgacc aggcgcaggc aacgctgaaa tcagcacagg ataaattgcg
     8581 tcagtaccgt tccggtaacc gtgaacagga catcgcccag gcgaaagcca gcctcgaaca
     8641 ggcgcaggcg caactggcgc aggcggagtt gaatttgcag gactcaacgt tgatagcccc
     8701 gtctgatggc acgctgttaa cgcgcgcggt ggagccaggc acggtcctca atgaaggtgg
     8761 cacggtgttt accgtttcac taacgcgtcc ggtgtgggtg cgcgcttatg ttgatgaacg
     8821 gaatcttgac caggcccagc cggggcgcaa agtgctgctt tataccgatg gtcgcccgga
     8881 caagccgtat cacgggcaga ttggtttcgt ttcgccgact gctgaattta ccccgaaaac
     8941 cgtcgaaaca ccggatctgc gtaccgacct cgtctatcgc ctgcgtattg tggtgaccga
     9001 cgccgatgat gcgttacgcc agggaatgcc ggtgacggtg caattcggta acgaggcagg
     9061 acatgaatga tgccgttatc acgctgaacg gcctggaaaa acgctttccg ggcatggaca
     9121 agcccgccgt cgcgccgctc gattgtacca ttcacgccgg ttatgtgacg gggctggtgg
     9181 ggccggatgg tgcaggtaaa accacgctaa tgcggatgct ggcgggatta ctgaaacccg
     9241 acagcggcaa tgccacggtg attggctttg atccaatcaa aaacgacggc gcgctgcacg
     9301 ccgtgctcgg ctatatgccg cagaaatttg gtctgtatga agatctcacg gtgatggaga
     9361 acctcaatct gtacgcggat ttgcgcagcg tcaccggcga ggcacgtaag caaacttttg
     9421 ctcgcctgct ggagtttacg tctcttgggc cgtttaccgg acgcctggcg ggcaagctct
     9481 ccggtgggat gaaacaaaaa ctcggtctgg cctgtaccct ggtgggcgaa ccgaaagtgt
     9541 tgctgctcga tgaacccggc gtcggcgttg accctatctc acggcgcgaa ctgtggcaga
     9601 tggtgcatga gctggcaggc gaagggatgt taatcctctg gagtacctcg tatctcgacg
     9661 aagccgagca gtgccgtgac gtgttactga tgaacgaagg cgagttgctg tatcaggggg
     9721 aaccaaaagc cctgacacaa accatggccg gacgcagttt tctgatgacc agtccacacg
     9781 agggcaaccg caaactattg caacgcgcct tgaaactgcc acaggtcagc gacggcatga
     9841 ttcaggggaa atcggtacgt ctgatcctca aaaaagaggc cacaccagac gatattcgcc
     9901 atgccgacgg gatgccggaa atcaacatca acgaaactac gccgcgtttt gaagatgcat
     9961 ttattgattt gctgggtggt gccggaacct cagaatcgcc gctgggcgca atattacata
    10021 cggtagaagg cacacccggc gagacggtga tcgaagcgaa agaactgacc aagaaatttg
    10081 gtgattttgc cgccaccgat cacgtcaact ttgccgttaa acgtggggag atttttggtt
    10141 tgctggggcc aaacggcgcg ggtaaatcga ccacctttaa gatgatgtgc ggtttgctgg
    10201 tgccgacttc cggccaggcg ctggtgctgg ggatggatct gaaagagagt tctggtaaag
    10261 cgcgccagca tctcggctat atggcgcaaa agttttcgct ctacggtaac ctgacggtcg
    10321 aacagaattt acgctttttc tctggtgtgt atggcttacg cggtcgggcg cagaacgaaa
    10381 aaatctcccg tatgagcgag gcgttcggcc tgaaaagtat cgcctcccac gccaccgatg
    10441 aactgccatt aggttttaaa cagcggctgg cactggcctg ctcgctgatg catgaaccgg
    10501 acattctgtt tctcgacgaa ccgacgtccg gcgttgatcc gattactcgc cgcgaattct
    10561 ggctacatat caacagcatg gtagagaaag gcgtcacggt gatggtcacc acccacttta
    10621 tggatgaagc agaatattgc gaccgcatcg gcttggtgta ccgtgggaaa ttaattgcca
    10681 gcggcacgcc ggatgatttg aaagcgcagt cggccaacga cgagcaaccc gatcccacca
    10741 tggagcaagc ctttattcag ttgatccacg actgggataa ggagcatagc aatgagtaac
    10801 ccgatcctgt cctggcgtcg cgtacgggcg ctgtgcgtta aagagacgcg gcagatcgtt
    10861 cgcgatccga gtagctggct gattgcggta gtgatcccgc tgctactgct gtttattttt
    10921 ggttacggca ttaacctcga ctccagcaag ctgcgggtcg ggattttact ggaacagcgt
    10981 agcgaagcgg cgctggattt cacccacacc atgaccggtt cgccctacat cgacgccacc
    11041 atcagcgata accgtcagga actgatcgcc aaaatgcagg cggggaaaat tcgcggtctg
    11101 gtggttattc cggtggattt tgcggaacag atggagcgcg ccaacgccac cgcaccgatt
    11161 caggtgatca ccgacggcag tgagccgaat accgctaact ttgtacaggg gtatgtcgaa
    11221 gggatctggc agatctggca aatgcagcga gcggaggaca acgggcagac ttttgaaccg
    11281 cttattgatg tacaaacccg ctactggttt aacccggcgg cgattagcca gcacttcatt
    11341 atccccggtg cggtgaccat tatcatgacg gtcatcggcg cgattctcac ctcgctggtg
    11401 gtggcgcgag aatgggaacg cggcaccatg gaggctctgc tctctacgga gattacccgc
    11461 acggaactgc tgctgtgtaa gctgatccct tattactttc tcgggatgct ggcgatgttg
    11521 ctgtgtatgc tggtgtcagt gtttattctc ggcgtgccgt atcgcgggtc gctgctgatt
    11581 ctgtttttta tctccagcct gtttttactc agtaccctgg ggatggggct gctgatttcc
    11641 acgattaccc gcaaccagtt caatgccgct caggtcgccc tgaacgccgc ttttctgccg
    11701 tcgattatgc tttccggctt tatttttcag atcgacagta tgcccgcggt gatccgcgcg
    11761 gtgacgtaca ttattcccgc tcgttatttc gtcagcaccc tgcaaagcct gttcctcgcc
    11821 gggaatattc cagtggtgct ggtggtaaac gtgctgtttt tgatcgcttc ggcggtgatg
    11881 tttatcggcc tgacgtggct gaaaaccaaa cgtcggctgg attagggaga agagcatgtt
    11941 tcatcgctta tggacgttaa tccgcaaaga gttgcagtcg ctgctgcgcg aaccgcaaac
    12001 ccgcgcgatt ctgattttac ctgtgcttat tcaggtgatt ctgttcccgt tcgccgccac
    12061 gctggaagtg actaacgcca ccatcgccat ctacgatgaa gataacggcg agcattcggt
    12121 ggagctgacc caacgttttg cccgcgccag cgcctttact catgtgctgc tgctgaaaag
    12181 cccacaggag atccgcccaa ccatcgacac acaaaaggcg ttactactgg tgcgtttccc
    12241 ggctgacttc tcgcgcaaac tggatacctt ccagaccgcg ccgttgcagt tgatcctcga
    12301 cgggcgtaac tccaacagtg cgcaaattgc cgccaactac ctgcaacaga tcgtcaaaaa
    12361 ttatcagcag gagctgctgg aaggaaaacc gaaacctaac aacagcgagc tggtggtacg
    12421 caactggtat aacccgaatc ttgactacaa atggtttgtg gtgccgtcgc tgatcgccat
    12481 gatcaccact atcggcgtaa tgatcgtcac ttcactttcc gtcgcccgcg aacgtgaaca
    12541 aggtacgctc gatcagctac tggtttcgcc gctcaccacc tggcagatct tcatcggcaa
    12601 agccgtaccg gcgttaattg tcgccacctt ccaggccacc attgtgctgg cgattggtat
    12661 ctgggcgtat caaatcccct tcgccggatc gctggcgctg ttctacttta cgatggtgat
    12721 ttacggttta tcgctggtgg gattcggtct gttgatttca tcactctgct cgacacaaca
    12781 gcaggcgttt atcggcgtgt ttgtttttat gatgcccgcc attctccttt ccggttacgt
    12841 ttctccggtg gaaaacatgc cagtatggct gcaaaaccta acgtggatta accctattcg
    12901 ccactttacg gacattacca agcagattta tttgaaggat gcgagtctgg atattgtgtg
    12961 gaatagtttg tggccgctac tggtgataac ggccacgaca gggtcagcgg cgtacgcgat
    13021 gtttagacgt aaggtgatgt aacttcttat ctttcgccag caaagacact accgccgggc
    13081 cggcaaggat tgccagccct gcaattgcca gcaaaaagtt gttttccagt acacgggaca
    13141 acagccacag cacaatcagc gctgcgccaa aataccaggt cgtggtcagc tcttccagca
    13201 cgtctgccac ctcacgccag cgttgctcgg ggtggcgctg aaacaccaac atcatcacta
    13261 ccgtgacaca atacagtgcg cataatccca cgccgacgtg aaccagagtc ttactcatcc
    13321 agcccaccac cagcagcgcc actaccagta aatgcaacat aatctgccag caacttagac
    13381 cagttgcgac acgaacacgt tgttgccact tcatggcttc tctcctgaag aatttatctc
    13441 tacttactca gagtaccgaa ctttacggaa aattccgtaa gaccgcacct ttttgtgacg
    13501 ccgactacac tatttcaatg agataagagg caaagacagg aaaattatgc ccgatcaaac
    13561 acaacaattt tcgttcaagg tgctcaccat caacattcac aaaggtttta ccgcgtttaa
    13621 ccgacgcttc attttgccgg aacttcgcga cgcggtgcgt accgtcagcg ccgatattgt
    13681 ttgcctgcag gaagtgatgg gcgcgcacga agttcatccg ctgcatgtgg aaaactggcc
    13741 cgatacctcg cactacgagt ttctcgccga cacgatgtgg agcgattttg cctacggtcg
    13801 caatgccgta tacccggaag ggcatcacgg caacgccgta ctgtcgcgtt atcccattga
    13861 acattatgag aatcgcgatg tttcggtcga tggtgcggaa aagcgcggcg tgctctactg
    13921 ccgcattgtg ccaccgatga ccggaaaagc gattcatgtg atgtgcgtac atctgggcct
    13981 gcgtgaggcg caccgtcagg cgcagcttgc gatgctcgcc gaatgggtga atgagctacc
    14041 ggacggcgaa ccggtattgg tggcgggtga tttcaatgac tggcggcaaa aagctaatca
    14101 tccgttaaaa gtgcaggccg gactggatga gatttttacc cgcgcccacg gacgcccggc
    14161 gcgcacgttt ccggtgcaat ttcctctact acgactggac aggatctacg tcaaaaatgc
    14221 cagcgccagc gcgccaaccg cgttgccgct gcggacatgg cgacaccttt ctgatcatgc
    14281 ccctttaagt gcggagattc atttatgaaa tgtagctggc gcgaaggcaa taagatccag
    14341 ttgctggaaa acggcgagca atattatccc gcggtgttta aggcgattgg tgaggcacaa
    14401 gaacgcatca ttcttgaaac gtttatctgg tttgaggatg acgtcggcaa acagctgcat
    14461 gcggcactac tggcagcagc gcaacgcggg gttaaagcgg aagtcttgct ggatggctac
    14521 ggttcgccgg atctcagcga tgagtttgtc aatgaactga cggcagctgg cgtggtattt
    14581 cgctactacg atccccgccc tcgccttttt ggtatgcgca ccaatgtgtt tcgccggatg
    14641 catcgcaaaa ttgtggtgat cgacgcgcgt atcgccttta ttggcgggct gaattactcc
    14701 gccgagcata tgtccagcta cggtccagag gctaaacagg attacgcggt acgccttgaa
    14761 gggccgattg tcgaagatat tctccagttt gagctggaaa acctgcctgg acagagcgcg
    14821 gcacgacgct ggtggcgacg tcatcacaaa gcggaagaga accgccagcc gggagaagcg
    14881 caggtattgc tggtctggcg cgataacgaa gaacatcgcg atgatattga acgtcactat
    14941 ctgaaaatgc tcactcaggc gcggcgagaa gtcattatcg ccaacgccta cttcttcccc
    15001 ggctatcgat ttttacacgc cttgcgtaaa gcggcacggc gcggggtgcg gatcaaactg
    15061 atcattcagg gcgaaccgga tatgccgatt gtcagagtcg gtgcgcgctt gctgtataac
    15121 tatctggtta aaggcggcgt tcaggtattt gagtaccgcc gccgcccgct ccacggcaaa
    15181 gtggcattga tggacgatca ctgggcgaca gtagggtcca gtaatctcga tccgctcagt
    15241 ttgtcactga atctcgaagc aaatgtcatc atccacgatc gtcattttaa ccagacgctg
    15301 cgcgataatc tgaacggcat tattgccgca gattgtcagc aggtcgatga gaccatgctg
    15361 ccgaaacgca cctggtggaa cctgaccaaa agcgtgctgg cgttccactt tttacgccac
    15421 ttcccggcgc tggttggctg gcttccggca cacacaccac gtctggcgca ggttgatccg
    15481 cccgcacaac cgacaatgga aacgcaggat cgggtagaaa ctgaaaacac gggggtaaaa
    15541 ccctgatgag taaatcacac ccacgctggc gcttagcaaa gaagatcctc acctggctgt
    15601 tttttatcgc ggtgattgtg ttgctggtgg tctacgccaa aaaagtggac tgggaagagg
    15661 tctggaaggt catccgcgac tacaatcgcg ttgcgctgct tagtgcggtc gggctggtgg
    15721 tcgtcagcta tctgatttac ggctgctatg acctgctcgc ccgcttttac tgcggtcaca
    15781 aactggcgaa gcgccaggtg atgctggtgt cgtttatctg ctacgccttc aacctgacgc
    15841 tcagtacctg ggtcggcggc attggtatgc gctatcgttt gtactcccga ctagggttac
    15901 cgggcagcac tattacgcgg attttctcac tcagtattac caccaactgg ctgggctata
    15961 ttttactggc agggattatc tttaccgcag gcgtggtgga gttgccggat cactggtatg
    16021 tcgatcaaac tacgctgcgt attctcggca ttggcttact gatgattatc gcggtttatt
    16081 tgtggttttg cgctttcgcg aagcaccgcc atatgaccat caaagggcaa aagctggtgc
    16141 tgccttcatg gaaattcgcc ctcgctcaga tgctgatttc cagcgttaac tggatggtaa
    16201 tgggggcgat tatctggctg ttacttggtc aaagcgtgaa ctatttcttt gtactgggcg
    16261 tgttactggt tagtagtatt gctggcgtca tcgtgcatat tccggcgggg atcggtgtgc
    16321 tggaagcggt gtttatcgca ctactggctg gggagcatac ctcgaaaggg agcattatcg
    16381 ccgccctgct cgcttatcgc gtcctgtatt actttatccc gctgctgctg gcgctgatct
    16441 gttacctgct actggaaagc caggcgaaga agctacgggc gaaaaatgaa gcggcgatgt
    16501 gaagtttagt taagttcttt agtatctgca tttacgacta atgaaaaaaa cgcgtatgcc
    16561 tttgccagac aagcgttata gctatcgcgg caagtatccc aaagaagccg ataaaatagt
    16621 aatagagtgt taatgcacct gtaacagcgc aacgaaccgg actccacaac tcttcactat
    16681 ggcatttccc cgccaattca aggagcttat ttcgtccatg cgagataatg cctgaccata
    16741 acacaatcat taagcaactt attacccaaa cgggtcgttc actcattaat gcggcattca
    16801 tgatgatcac cagggccaac cctgtcaacg tcatcataat gataaaacgg tgagaaccag
    16861 gatcaacatt aaataataaa ccggccagca tactgattaa aaacatcgcg cccgcagtgc
    16921 tgaacacgac gataatggca ggtacaggag ctaaacttgc aacgacaatc gccaccaaag
    16981 agagtaaaaa cagatatatt acgcctgcga tgacggcatt tatcttgttg atatccttac
    17041 ttaagccata catacaaact aataataacc ctatggcgac atacattgtt ggtgaatcat
    17101 atttggttgc cgtaatttcg agatacttag ctccccaggc cagtcctgcg gttgccacca
    17161 aacctaaagc ggtaatgagc catataccgt ttaatatcct ggtctcgtgc tgaaaatcta
    17221 atttattgct gttttgtgag tatgattcca ttcttttagt tgtccttaga aaaaatatta
    17281 atattcacca tcaataaatt taaaaccacg catacaaaat acttttgttt ttatggatac
    17341 ggaaaacgct tccgctcgcc ataaggcgta ttttatacct ggtacttgtt aagtgtcgtt
    17401 tttagtaaga aaaaaaatcc cgacacatgg ccgggatatt gatggtgaaa aagaattaac
    17461 ggcggttgcc aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc agatacaagg
    17521 ttaacgcgcc aagaatggaa tatttgcgca ggttcgacgt gtcgcgggta tcaatctgct
    17581 cacccatatt tttcaatttc tgagtgtcat acgccgtcaa tccgacaaag acaatcacgc
    17641 cgatgtaggt aactgcccac atcaatgctt cgcttttcag ccagaagttg accagcgatg
    17701 ccagcacaat gccgattaac gccataaaca gcatattgcc aaagccactt aaatcgcgct
    17761 tcgtggtgta accgtacagg ctcattgcgc cgaacatccc ggcagtaacg acgaaagtac
    17821 tggcgataga agcagcggta tagacaatga atatactgga aagcgtaaga cccgtcagcg
    17881 ccgaataaag cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg
    17941 ataacacaat aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag
    18001 taaacaacag ctccatcacg gccgcggaat tagccgcata ccaggcaaca aatgcggtca
    18061 gcaacaagcc aacggtcatc cagccataga cttgcgccat ataagtttgc aagccagccc
    18121 ggggttgtac gattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata
    18181 aggaatatct taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca
    18241 tgtactgagc ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaaactac
    18301 cagcgttttg ccgcctgctg atcgctctcc cgagcttcaa cccagcggtc gccttccggc
    18361 gtggcttcgc gcttccagaa cggtgcgcgg gttttgagat aatccataat aaactgcccg
    18421 gcttcaaacg cactgctgcg atgcgcactg gtgacaccga caaaaacgat ttcatcgccc
    18481 ggccataatt ccccgatgcg gtgaatcaca gtgacgcgcc ccagcggcca gcggttacgc
    18541 gcttcatcaa caatttctgc cagtgctttt tcagtcatcc ccggatagtg ttcgagggtt
    18601 aatgctttga cgctgtcgcc caggttatgg ttgcgcacct taccagtaaa ggtgactacc
    18661 gcaccgtctt cgtcacgctc cgccagccac gggtactctt ctcctacgct gaacggctgc
    18721 ggaccaacaa caattttggt ttctgccatc ttaacctccg gttaccggcg ggaagaaagc
    18781 tacttcgtcg ccgtcagtca gcggatggtc aaaactcacc agcgtctggt tgacggcagc
    18841 cagtaatttg ccatcttcca gcgccagcgc ccagcgatcg ctctgcgcag ccatgtgctg
    18901 gcgtaacgct tcaacagttg ggaaatccgc agccacttcg gttgcatctg ttcccaccaa
    18961 ctcgcgcacc tgggcgaaaa aaagaacttt aatcatccgc ttccacctta aagtcacccg
    19021 acttgccgcc gctcttcgcc agcaaacgta ccggaccaat caccatatct ttttgcaccg
    19081 ctttgcacat gtcataaatg gtcagcgccg ccacggaggc cgcggttaat gcttccattt
    19141 cgacaccggt tttcccggtc aggcggcata aggtttctat acgcacccga ttgtgctccg
    19201 gctcggcctg taaattgact tcaactttgc tgagcatcag cggatgacag agcgggatca
    19261 gatcccaggt gcgttttgcc gcctgaatac cggcaatacg cgcagtggca aatacgtcgc
    19321 ctttgtggtg gcgaccatca ataatcatcg ccagcgtctc gctgcgcatg gtgacaaagg
    19381 cttccgcccg cgcttcacgc acggtttccg ctttggcgga gacatccacc atgtgcgctt
    19441 cgccagcggc gttgatatgg gtcagttgcg acatacttat ttcttcaaat gtggatggaa
    19501 attacacgga cgcgtacggg catccagctg cggcgcgatg atattttccc atgcggtacg
    19561 gcacgctttg gtcgaacccg gcatggcgaa aatcagcgtt ttgttggcga cgcccgctac
    19621 cgcacgagat tgcaacgtgg aagtgccaat ctcttcaaac gacaacatac ggaacacttc
    19681 accaaaacct tcaatttcac ggtcgaacaa cggcagcaat gcttcgggag cctgatcacc
    19741 ttccgtcagg ccagtaccac ccgtaatcaa taccacttgt acatcgtcgc tggcgatcca
    19801 cgcagatacc tgagcgcgaa tagcgtagcg gttttctttc acaatggctt tatcgacaac
    19861 gtgatggccc gcttcttgcg ccgaatcgcg cagatagtga ccggaggtat cgtcttcttc
    19921 accgcgacga ttagaaaccg taagaatagc aatacgggtc gggataaatt cagtgcttac
    19981 ctgactcatc tgatctctcc ttttgacgtt ttagccgcca atgtacgata agttttgcgt
    20041 aataccggtg ttgttttgat gcaggaaatg ggtctgtttc ttctcccgca gcgccgctga
    20101 aatacgcgct tccagcgcct gttgctgggt atcgtcttcc agcagatcgc gcaggttaac
    20161 gccgccttca ccaaacaggc agagatggag tttaccaatg gaggaaacgc gcaggcggtt
    20221 gcaagtggcg cagaagtctt tttcatacgg catgataagg ccaatctctc cggcgtaatc
    20281 tggatggcaa aagacttgcg cgggaccgtc gctgcgttga cgtaattggt ggatccagcc
    20341 gcgacgcagt agctcgtcac gcagaacctg accagagatg tgatgcttac ggaagagctc
    20401 gctgccctcg cccgtttcca tcagttcgat gaaacgcagc tggatagggc gatgctggat
    20461 ccagttcaga aaggtgtcga gctggtgatg attaacatca cgcatcagca cggtattgac
    20521 tttgaccttc tcaaaaccgg cctcaaatgc agcatcaatc cccgccatga cctggttgaa
    20581 tttatcctgc ccggtaatag cgtgaaactg gcgggcgtcc agactgtcga cactgacgtt
    20641 aatgccagta agtcccgcat cgcgccagtt cgccacatcg cgttccagac ggtaaccatt
    20701 ggtggtgacc gcaatctggc ggatagcgtc gttttcccgc acagcggcga tgatatcggt
    20761 aaagtcgcgg cgtaaagacg gctcaccgcc tgtcaggcgc actttttcgg tgcccagact
    20821 ggcgaaggcg cgcgtaaccc ggcgaatttc atcgacggta agaaagcctt tattggtgac
    20881 gccgctcggt ttgtagccat ccggcaggca gtaggtgcaa cgaaagttac acacatcggt
    20941 aatcgacagg cgcaagtagt aaaacttacg cgcaaatgca tcagtcagtt gtgaagccat
    21001 gtacaccttt ccagatacgg gaggcgaagt catttcttcc ttcgccctgg tggcaatctt
    21061 tacgagcatt gtcacggcca aagcaccgta tcagttgacc caggtgcaga ggctagagtg
    21121 tttagtggtt atgccgatac tagcgtgtaa atgagatttt taccatccac actttcgcta
    21181 tgtaatcatg tatatagcgt catgatcgtg ctatttcgct acagaataag ggaaaatggc
    21241 ggttttactt tgatatacat catcagggag gcatagcgtg catcgtcctt tagaggtaga
    21301 agaaaatagt ttgtatcctc aatattggca ggttaattgc tgtttccccg caatttgcgc
    21361 tactgtagcg cggataatct gactccagga aactatatgc gcaatcgtac gctggctgat
    21421 cttgatcgtg tcgttgctct cggcggaggg catggactgg gacgcgttct ctcatcactt
    21481 tcgtctttgg gttctcgttt aacgggtatc gtcaccacca ccgataatgg tggctcgacg
    21541 gggcgtattc gccgttcaga aggcggcatt gcctggggcg atatgcgcaa ctgcctcaac
    21601 cagctgataa cggaaccgag cgtcgcctcc gcgatgtttg aataccgttt tggtggcaat
    21661 ggcgaacttt ccggtcataa tctcggaaac ttgatgttaa aggcgctgga tcaccttagc
    21721 gtgcggcctc tggaagccat caatttaatt cgtaatctgc tgaaagtgga tacgcatttg
    21781 attccaatgt cagagcatcc tgttgatctg atggcgattg acgatcaggg gcatgaagtt
    21841 tacggcgagg tcaatatcga ccagttaact acgccgattc aagagttatt gttaacgcct
    21901 aatgtacccg caacgcgtga ggcggttcac gctatcaatg aagcggatct catcattatt
    21961 gggcctggca gtttttatac cagcctgatg ccaattctgc tgctgaagga aatcgcccag
    22021 gcattacgcc gcacgccagc gccgatggtt tatatcggca atctggggcg tgagttgagt
    22081 ttacctgcgg ctaatttgaa gctggaaagc aagctggcaa ttatggagca gtatgttggt
    22141 aaaaaggtca ttgatgcggt catcgtcggg ccaaaagtgg atgtctcggc ggtgaaagag
    22201 cggattgtga tccaggaggt actggaggcc agcgatattc cgtatcgtca tgaccgccag
    22261 ttgttacata acgcgctgga aaaggcgtta caggctttag gttaacggaa atccgaagga
    22321 aaattccggc ttcctattga agacaaagtg cgcgttgttt atgccggatg cggcgtgaac
    22381 gccttatccg gccaacaaac cgcgcaaatt caatatattg cggagaaaat gtaggcctga
    22441 taagcgtagc gcatcaggct gttttccgtt tgtcatcagt cttcttcgct atcctgttac
    22501 gatgccgcga taaacagctc acgcagctga tgcaactggt cacgaatttg cgccgcttct
    22561 tcgaactcca gattctgcgc gtgttgcatc atcaaccctt ccagctcatg gattttctgc
    22621 tgcaacgctt taggcgacat atccatcggc acattatccg gctcaacaat cggtcgcgat
    22681 tttcctctgc ccttcgcttt ggttttagca atgttctgcc ccaacgccag gatatcgacc
    22741 actttcttgt tcaagccttg cggcgtaatg ccgtgttcct cgttgtactt ctgctgtttc
    22801 tcgcggcgac gttcggtttc gccaatcgct ttcgccattg atggggtgat cttatcgccg
    22861 tagagaatcg ctttaccgtt aacgttacgt gccgcacgac caatggtctg gatcaacgaa
    22921 cgttcggaac gcaggaagcc ttctttgtca gcgtcgagga tcgccaccag cgaaacttcc
    22981 ggcatatcca gaccttcacg cagtaagttg atccctacca atacgtcgaa ctcacccaga
    23041 cgcaagtcgc ggataatctc catacgctcg acggtgtcga tatctgagtg aagataacgc
    23101 acgcgctcac cgtgttcttc gagatattcg gtaagatctt ccgccattcg cttggtcagt
    23161 gtggtgacca gtacgcgttc gttaattgcc gctcgctgac gaatctccga aagaagatca
    23221 tcaacctgtg tcgccaccgg ccgcacttcg ataatcgggt caagcaatcc ggttggacgc
    23281 accacctgat ccaccacatc gccgccggat ttttccagct cgtaattacc cggcgtcgcc
    23341 gaaacataga tggtttgcgg cgctaatgct tcgaactctt caaacttaag cggacggtta
    23401 tccagcgctg atggcaggcg gaagccgtac tccaccagtg tctctttacg cgcccggtca
    23461 ccgcgataca tgccgccaat ttgtggaatg gtgacgtgag attcatcgac caccagcagc
    23521 ccatcagcag ggaggtaatc aaacagcgtc ggcggtggct cacccggtcc acgaccggag
    23581 aggaagcgcg agtagttttc aatccccgaa cagtagccca gctcgttcat catctccaga
    23641 tcaaactggg tacgctgggt cagccgctgc tcttccagca gtttgttgtt ttccaacagc
    23701 actttgcgtc tggcggccag ctcttctttg atctcctcca tcgcctgtac gatgcgctcg
    23761 cgcggtgtga cgtagtgcgt tttcgggtag atggtaaaac gtggaatagt ggaaacaatc
    23821 tgcccggtca gcgggtcaaa taacgacaat cgttccactt cctcgtcaaa cagttccacg
    23881 cgaagtgcaa tgtcatccga ttctgccggg aagatatcta tcacctcgcc acgaacgcgg
    23941 aaagtaccac gctggaatgc ttgatcatta cgagcgtatt gcagctccgc cagtcggcgc
    24001 agaatcgcgc gctgatcgat aatcataccg accgtgagat ggagcatcat cttgagatat
    24061 aaatcaggat cgcccagacc ataaatcgcg gaaacagacg ccaccacaac cacatcacgc
    24121 cgctccagca tcgctttggt ggcggacaaa cgcatctgct caatatgttc gttaaccgag
    24181 gcatctttct caatgaaagt gtcggaactc ggtacatagg cttccggctg atagtagtcg
    24241 tagtaggaaa cgaaatattc caccgcgttt tccgggaaga actctttcat ttcgccatac
    24301 agctgggccg ccagcgtttt gttgggcgca agtaccatgg ttgggcgctg aaggtcagca
    24361 atgacattgg caatggtgaa tgttttcccg gagccagtca cgccaagtaa cgtctggtgc
    24421 gccaggccat cttccagccc ctcttcgaga cgtcgaatcg cctctggctg atcgccagaa
    24481 ggtttaaaag cggaattcag tttgaacggt ttactcatga gtcgctacct gaaggagttg
    24541 ggcgggcagg tatgtaattt tactcgtcgt acttaattat gccaacaaat tatactggat
    24601 aaaaaaacag ttcatcacca taatatttct gttacagcgt aaactccgct gtcaatcatg
    24661 agcaaaattt actctgtggc gagataaaac tccggcctta ccgggttatc cccaaagcaa
    24721 cggctttttt aacatttgtc aagatgagtg atgacagttt tatggcaaga gatgcctgtt
    24781 cagtgactgc cattgcattt atctaaccag ttaaaaaata aaagatattt ctttgagccg
    24841 tctttaacgc caattcgcgt gcaagccgcg tattctctcg cttgcctcgt gttttctaac
    24901 tctaatacac atggttatcc acaggaatag tggataactg tctccagccc ctatcccccg
    24961 ccgcctgggc aattcccaca ttcgccgcat gaggcggaag taaaattttt ttcgtgatta
    25021 ttttagaatt taattggtta aattgcagtc aatcgaagac gcgatctcgc tcgcaattta
    25081 accaaataca ggatagctac aacaaggcaa ggtttatgta ttttccggtt gccgcatttt
    25141 ctggattttc tgcaagccag gggatctctc ccagcagcgg cgcgggaatc atgcgggtga
    25201 gcgtggtcat atattcagcg tgacgttttc ccggaggcgt aacatcgttc gccacccaac
    25261 ccgccagagt cagtccggcg tgttgtattg cctgcgcagt caacatcgcg tgattaatac
    25321 agccgagttt cacaccaact accagtatca ccggcagttg ttcctgtgtt acccaatctg
    25381 caaaagtgaa agtgtcagaa agcggcgtaa accagccgcc agcaccttcc actaacaccc
    25441 agtcagcctg ttgttcaagc gcgcgtaatc cggcgctcat taccaatgat tctatcggcc
    25501 tgccctcttg cgcgctgatg atgtgcggcg aggtgggttc tgcgaaggtg taaggattta
    25561 ccgttgcgta atccagctgc aggctgctgt tgtgctgtaa cgccagcgcg tcactattac
    25621 gtaagccttc cggggtcttt tcgctgccag aggcgaccgg tttataacct gccgtccgat
    25681 aacctactgc cttcgcggct tgtaaaagtg cacaactggc gacggttttc cccacttcgg
    25741 tatccgttcc ggtgacaaaa taacgtttac tcacgagcaa tcactcccaa aaaaagatga
    25801 tacgtcagag gatatcgccc ctgttgttgc ggccaggcca gttgcaatcg ctgcaattgc
    25861 gaacgcgtta atattcgcgg gtcgcgccct tcatgaagat gcgtagcacc gatgcctttc
    25921 agcgaacgca tggcactgag cgcatcatca aaccatagtg tgatgggctg aatatgatgt
    25981 tgataatgca cgccgttcag cgactgttcg atttcatctg gcggtaaaaa gcgattagca
    26041 tgcggacgct cgtccaccgc ctgccacgcc tgatgcagtt cgggtaacga tccctgcacc
    26101 agcgtggtaa acgccaccac gcctccgggg cgcaccaccc gatacagctc gcggagtgca
    26161 gtggataaat taccgcacca ctgcactgcg agattgctcc atgcaagatc gaacgtcgca
    26221 gtcgctaacg gcagggattc gatatctccc gccagataat ggtctgcggc atccttctgg
    26281 cgtgcctgaa caagcattgg cggcgagaga tctaaggccg tcacctgcgc gtgacgttcc
    26341 cgccagtggc ggctcatcca gccaggtcca caacccgcgt ccagtacgtg ggtgtattta
    26401 cgctgtggga gcattgccag taagacgtca gcactctggc gctgtagatc tgcgtgttgc
    26461 tcatagagtg cggctgcccg accaaatgcc gctgcaatgg cttgtttatt aaccgttgcc
    26521 atgcagcacc tccagcagac ggtcgatatc ctgcatttca tgcgctgcgg ttagcgttaa
    26581 gcgcagtcgc gcagtaccag cgggtacggt tggcgggcga atcgccgtga cccagcagcc
    26641 ttgctggcgc agtttttctg ccagttgtaa cgcacggctg ttatcaccga cgatcaatgg
    26701 ctggatggcg ctgcatgaat cagccagcgt aaacggcaaa tcctgtactc cggcacgaaa
    26761 acgcgcaatg agtgacacca gtttttcgcg ccgtgcatcc ccctcatcac ggcgaatgac
    26821 cgccagtgac gcacgtaatg cctgcgcctg agcgggcggc atactggtgc tgtagataag
    26881 gtggcgggcg aattgcagca gataatccgc caccgtactg gagcaaagca ccgcagcccc
    26941 actgacgcca aatcctttgc caaaagtcac caccagcaat tctggtttta ccttttgcag
    27001 ccagcagctg ccgcgcccct gctccccgat aacgcccgtg ccgtgggcat catcgaccat
    27061 caaccagcca ttgtgctgtt gcgttacctg ctggatttcc gccagtggcg caatatcgcc
    27121 gtccatgctg aacacgcctt ctgtcaccac cagttgctgc cccggacagg gggaagcaag
    27181 cagtcgcgcc agatgagtga catcgttatg aacaaaacgg cgaagctgcg acgggcttaa
    27241 actggcagcc tccagcaatg aggcatggct aagccggtca gcgacaatgc ggtcctcttt
    27301 tgccatcatc gcggcaatga ccgcctgatt agcggcaaaa ccagagataa atagcagtgc
    27361 ccgcgaatag cccagccact cggccagttc ttcttccagc gcctgatgcg caacgctata
    27421 accgctgacg tgaccggaac cgccgctacc gacgccaaat tgctccgcac cctgctgcca
    27481 ggcacggata atttgcggat gatggcttaa acctaaataa tcgttactgg aaaagttcag
    27541 atactggcga tcatccgcca ccagccagcg tccggctcct tgcgccactg gataacggcg
    27601 acgcagggca tcggcagcac gccgcgcatc gagcgccgcg ttgattttct cctgccagct
    27661 cataatgctg ccgcgttgta atattcgtcg gtgtccgggg tcatcagcgc ctgttcaaga
    27721 cgttgctgtt gttcgttatc ccctgccagc acggcagttt gctgcggatt tagccccagt
    27781 ttgcggaaca gttgcaggtc tttatcttct tccggattcg gcgtggtcag cagtttgcaa
    27841 ccgtagaaaa tcgagtttgc gcctgccata aagcacatcg cctgagtctg ttcgttcatc
    27901 tgctcgcgtc cggcagaaag gcgcacgtaa gaggtcggca tcatgatccg cgcgaccgca
    27961 atggtgcgaa taaaatcaaa ggcatcgaca tcatcgttat ccgccagcgg cgtgcctttc
    28021 accttcacca gcatgttgat tggtacgctt tccggcggcg tcggcaggtt tgccagttgc
    28081 agcaataatc cggcgcgatc ttttaccgtt tcgcctaagc ccacaatgcc gccagaacag
    28141 actttgatcc cggcatcgcg cactttttcc agcgtatcga ggcgttcctg ataagtgcgg
    28201 gtggtgatga tattgccgta aaactccggc gaggtgtcga ggttgtggtt gtagtaatcc
    28261 agcccggcat tcgcaaggcg ctgcgcctga gattcactca acgtgcccag cgtcatacac
    28321 gcctccagcc ccatcgcttt taccccctgc accatttgtt ccaggtacgg catatcgcgt
    28381 tcgtggggat tcttccacgc cgcgcccata cagaaacgcg tcgatcccgc agctttcgct
    28441 ttgcgcgccg actccagcac ctgttcaact tccatcaacc gctcggcttc cagcccggtt
    28501 ttgtagcgcg agctttgcgg gcagtatttg caatcttccg gacaagctcc ggtcttaatc
    28561 gacagcaaag tgctgacctg cacctgacga ggatcgaaat gctgtcgatg cacctgctgc
    28621 gcttcaaaca gcagatccag caacggtttt tcaaataatt ctgtgacttg cgacaatgtc
    28681 cagcgtgggc ggtgagccat ggggcttctc caaaacgtgt tttttgttgt taattcggtg
    28741 tagaattgta aacctaaatc ttttcaattt ggtttacaag tcgattatga caacggacga
    28801 tcttgccttt gaccaacgcc atatctggca cccatacaca tccatgacct cccctctgcc
    28861 ggtttatccg gtcgcgagcg ccgaaggttg cgagctaatt ttgtctgacg gcagacgcct
    28921 ggttgacggt atgtcgtcct ggtgggcggc gatccacggc tataatcacc cgcagcttaa
    28981 tgcggcgatg aagtcgcaaa ttgatgctat gtcgcatgtg atgtttggcg gtatcaccca
    29041 tgcgcccgcc atcgagctat gccgcaaact ggtggcaatg acgccgcaac cgctggaatg
    29101 cgtttttctc gcggactccg gctccgtagc ggtggaagtg gcgatgaaaa tggcgttgca
    29161 gtactggcag gccaaaggcg aagcgcgcca gcgttttctg accttccgca atggttatca
    29221 tggcgatacc tttggcgcga tgtcggtgtg cgatccggac aactcaatgc acagcctgtg
    29281 gaaaggctat ctgccagaaa acttgtttgc tcccgccccg caaagccgca tggatggtga
    29341 atgggatgaa cgtgatatgg ttggctttgc ccgcctgatg gcggcgcatc gtcatgaaat
    29401 cgcggcggtg atcattgaac cgattgtcca gggcgcaggc gggatgcgca tgtaccatcc
    29461 ggaatggtta aaacgaatcc gcaaaatgtg cgatcgcgaa ggtatcttgc tgattgccga
    29521 cgagatcgcc accggatttg gtcgtaccgg caaactgttt gcctgtgaac atgcagaaat
    29581 cgcgccggac attttgtgcc tcggtaaagc cttaaccggc ggcacaatga ccctttccgc
    29641 cacgctcacc acgcgcgagg ttgcggaaac catcagtaac ggcgaagccg gttgctttat
    29701 gcatgggcca acttttatgg gcaatccgct ggcctgcgcg gcagcaaacg ccagcctggc
    29761 gattctcgaa tctggcgact ggcagcaaca ggtggcggct attgaagtac agctgcgcga
    29821 gcaacttgcc cccgcccgtg atgccgaaat ggttgccgat gtgcgcgtgc tgggggcaat
    29881 cggcgtagtc gaaaccactc atccggtgaa tatggcggcg ctgcaaaaat tctttgtcga
    29941 acagggtgtc tggatccggc cttttggcaa actgatttac ctgatgccgc cctatattat
    30001 tctcccgcaa cagttgcagc gtctgaccgc agcggttaac cgcgcggtac aggatgaaac
    30061 atttttttgc caataacgag aagtccgcgt gagggtttct ggctacactt tctgcaaaca
    30121 agaaaggagg gttcatgaaa ctcatcagta acgatctgcg cgatggcgat aaattgccgc
    30181 atcgtcatgt ctttaacggc atgggttacg atggcgataa tatttcaccg catctggcgt
    30241 gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac ctgctacgac ccggatgcgc
    30301 caaccggctc cggctggtgg cactgggtag ttgttaactt acccgctgat acccgcgtat
    30361 taccgcaagg gtttggctct ggtctggtag caatgccaga cggcgttttg cagacgcgta
    30421 ccgactttgg taaaaccggg tacgatggcg cagcaccgcc gaaaggcgaa actcatcgct
    30481 acatttttac cgttcacgcg ctggatatag aacgtattga tgtcgatgaa ggtgccagcg
    30541 gcgcgatggt cgggtttaac gttcatttcc actctctggc aagcgcctcg attactgcga
    30601 tgtttagtta atcactctgc cagatggcgc aatgccatct ggtatcactt aaaggtatta
    30661 aaaacaactt tttgtctttt taccttcccg tttcgctcaa gttagtataa aaaagctgaa
    30721 cgtgaaacgc taaagaccat taatatcaat gtgttacaat atatttagtc taaaaaatag
    30781 actgcataat actacaaaac acaacatatc tagtaactat gaatcaacta cttagatagt
    30841 attagtgacc tgagacaga
//
LOCUS       DAZGTR010000048        30281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGTR010000048 DAZGTR010000000
VERSION     DAZGTR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.048"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            147..1730
                     /gene="yegH"
                     /locus_tag="ACHLCY_002371"
     CDS             147..1730
                     /gene="yegH"
                     /locus_tag="ACHLCY_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0369618.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLTLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(2004..3857)
                     /gene="asmA"
                     /locus_tag="ACHLCY_002372"
     CDS             complement(2004..3857)
                     /gene="asmA"
                     /locus_tag="ACHLCY_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP0369619.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDITSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3879..4460)
                     /gene="dcd"
                     /locus_tag="ACHLCY_002373"
     CDS             complement(3879..4460)
                     /gene="dcd"
                     /locus_tag="ACHLCY_002373"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP0369620.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDESEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4552..5193)
                     /gene="udk"
                     /locus_tag="ACHLCY_002374"
     CDS             complement(4552..5193)
                     /gene="udk"
                     /locus_tag="ACHLCY_002374"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP0369621.1"
                     /translation="MTEQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERIKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5511..8828
                     /gene="dgcE"
                     /locus_tag="ACHLCY_002375"
     CDS             5511..8828
                     /gene="dgcE"
                     /locus_tag="ACHLCY_002375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0369622.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESEPRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNIESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8937..9785)
                     /gene="alkA"
                     /locus_tag="ACHLCY_002376"
     CDS             complement(8937..9785)
                     /gene="alkA"
                     /locus_tag="ACHLCY_002376"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP0369623.1"
                     /translation="MYTLNWQSPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAVPDIARHTLHINLSVGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPSSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGSW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9919..11271
                     /gene="yegD"
                     /locus_tag="ACHLCY_002377"
     CDS             9919..11271
                     /gene="yegD"
                     /locus_tag="ACHLCY_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0369624.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPETITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLTRILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11284..13224)
                     /gene="yegI"
                     /locus_tag="ACHLCY_002378"
     CDS             complement(11284..13224)
                     /gene="yegI"
                     /locus_tag="ACHLCY_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP0369625.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFADSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYESDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLTDCPWCALDNQGVIY
                     FIDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRTMLEKMKDEMLGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKVAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13221..13982)
                     /gene="pphC"
                     /locus_tag="ACHLCY_002379"
     CDS             complement(13221..13982)
                     /gene="pphC"
                     /locus_tag="ACHLCY_002379"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP0369626.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFF
                     ADGAGSVSQGGEGAKLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGRGLELPLTPMVGEYANMTHFI
                     SDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13979..14638)
                     /gene="yegL"
                     /locus_tag="ACHLCY_002380"
     CDS             complement(13979..14638)
                     /gene="yegL"
                     /locus_tag="ACHLCY_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0369627.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSCSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14859..14918)
                     /gene="ibsA"
                     /locus_tag="ACHLCY_002381"
     CDS             complement(14859..14918)
                     /gene="ibsA"
                     /locus_tag="ACHLCY_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP0369628.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15192..15248)
                     /locus_tag="ACHLCY_002382"
     CDS             complement(15192..15248)
                     /locus_tag="ACHLCY_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0369629.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15520..15576)
                     /locus_tag="ACHLCY_002383"
     CDS             complement(15520..15576)
                     /locus_tag="ACHLCY_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0369630.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15855..17102
                     /gene="mdtA"
                     /locus_tag="ACHLCY_002384"
     CDS             15855..17102
                     /gene="mdtA"
                     /locus_tag="ACHLCY_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP0369631.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLVKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17102..20224
                     /gene="mdtB"
                     /locus_tag="ACHLCY_002385"
     CDS             17102..20224
                     /gene="mdtB"
                     /locus_tag="ACHLCY_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP0369632.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20225..23302
                     /gene="mdtC"
                     /locus_tag="ACHLCY_002386"
     CDS             20225..23302
                     /gene="mdtC"
                     /locus_tag="ACHLCY_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP0369633.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23303..24700
                     /gene="mdtD"
                     /locus_tag="ACHLCY_002387"
     CDS             23303..24700
                     /gene="mdtD"
                     /locus_tag="ACHLCY_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0369634.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIFARVPND
                     THQNVAISRRKRSAQ"
     gene            24697..26100
                     /gene="baeS"
                     /locus_tag="ACHLCY_002388"
     CDS             24697..26100
                     /gene="baeS"
                     /locus_tag="ACHLCY_002388"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP0369635.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26097..26819
                     /gene="baeR"
                     /locus_tag="ACHLCY_002389"
     CDS             26097..26819
                     /gene="baeR"
                     /locus_tag="ACHLCY_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP0369636.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27010..27342
                     /gene="yegP"
                     /locus_tag="ACHLCY_002390"
     CDS             27010..27342
                     /gene="yegP"
                     /locus_tag="ACHLCY_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP0369637.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27551..27847
                     /locus_tag="ACHLCY_002391"
     CDS             27551..27847
                     /locus_tag="ACHLCY_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0369638.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27849..28145
                     /locus_tag="ACHLCY_002392"
     CDS             27849..28145
                     /locus_tag="ACHLCY_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0369639.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28248..29609
                     /gene="yegQ"
                     /locus_tag="ACHLCY_002393"
     CDS             28248..29609
                     /gene="yegQ"
                     /locus_tag="ACHLCY_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP0369640.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29939..30256)
                     /gene="yegR"
                     /locus_tag="ACHLCY_002394"
     CDS             complement(29939..30256)
                     /gene="yegR"
                     /locus_tag="ACHLCY_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP0369641.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         7008 a         8073 c         7971 g         7229 t
ORIGIN      
        1 ttaccacttt taattcaggg ataatgtgag gttataaacc tcaaataata tgagataaat
       61 agtgctgcaa cattgcattt ttgccccgat ttatccatga tcgaattgtg acatttgtca
      121 tacaacgaat aggttttgta cttactatgg aatggattgc cgatccgtct atctgggccg
      181 ggttaatcac gctgattgtg atcgaactgg tcctcggcat tgataacctg gtctttattg
      241 ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt accgggctgc
      301 tgctggcgat gttaatgcgc ctgttactgc tggcgtcaat ctcctggctg gtcaccctga
      361 cacaaccgct attcagcttt cgctcgttta cctttagcgc ccgcgactta atcatgctgt
      421 ttggtggttt cttcctgttg ttcaaagcca cgatggagct gaacgaacgg ctggaaggga
      481 aagacagcaa taaccccacg caacgcaaag gtgcgaagtt ctggggggtg gtgacacaaa
      541 ttgtggtact ggacgccatc ttctcactcg actcggtgat taccgccgtc gggatggtcg
      601 accatttact ggtcatgatg gccgccgtcg ttatcgctat tagcctgatg ttgatggcca
      661 gcaagccgtt aacgcaattt gttaacagtc acccgacgat cgttattctc tgcttaagct
      721 tcctgttgat gattggcttt agcctggtgg cagaaggttt cggcttcgtc attccgaaag
      781 gctacctgta cgctgccatt ggtttctcgg tgatgatcga ggcgctcaat cagttggcta
      841 tctttaaccg gcgacgtttt ctttccgcta accagacgct gcgccagcgg actaccgaag
      901 cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga actggacgcc gaaaccgcgt
      961 ccatgctggt ggatcatggt aaccagcaga tctttaatcc gcaggaacgg cggatgattg
     1021 agcgggtact taatcttaac cagcgtactg tcagcagcat tatgacgtcg cgccacgata
     1081 ttgagcatat cgatctcaac gcgccggaag aagaaatccg ccagttgctg gagcgaaatc
     1141 agcatacgcg gttggtggtt accgacggtg atgacgcaga agatttgctc ggtgttgttc
     1201 acgttatcga cctgctacaa cagtctctgc gcggcgaacc gctcaacctg cgggtgttga
     1261 ttcgccagcc gctggtgttc ccggaaacct tgacgttgtt acctgccctg gagcagttcc
     1321 gtaatgcccg cacgcacttt gcttttgtgg tggatgagtt tggctcggtg gaagggattg
     1381 tgacattaag tgacgtcact gaaaccattg ccggtaactt accgaacgaa gtggaagaga
     1441 tcgacgcccg ccatgatatt cagaagaatg cggacggttc ctggacggcg aatggtcata
     1501 tgccgctgga agatctggtg caatatgtgc cgctgccgct ggatgaaaaa cgtgaatatc
     1561 acaccattgc cgggctgttg atggaatatt tacagcgtat tccaaaacct ggcgaagaag
     1621 ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga aagccatcgc gtgcagaagg
     1681 tgcagattat tccgctgcat aaggatggcg agttggagta cgaggtgtga tggtatcggt
     1741 cattttaccc aatagccgaa aggaataaga tgagggaacc gcgccagcgg cgatttaccg
     1801 ccgggagccc gggttgccag ggtggtggag attgagccac cctggcacgt tcaccggctt
     1861 gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat cttacatatt
     1921 cagaatataa tgatgccgcc acgaacgcct gatgcgacgc tttacgcgtc ttatcaggcc
     1981 tacaggactg gtcacctggt aacctacatc ttctccagca acttcttcac atccttgcca
     2041 ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc ttcgtcctgt
     2101 agatgtttgc gcagtaactg atccacttgc agactgtaat tgagttgctg ccagttgcca
     2161 taaacccgca gcggtactgg cgtttctttc agaaaatcaa tcagtttact ttccccgttc
     2221 cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc cagattcaac
     2281 atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt taacgtcacg
     2341 acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac gttatcaaag
     2401 ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat catctgctgg
     2461 aagttcatcc cttccatgcg cgtgtcggtc atttcgacat gtgcctgccc ttgccagttg
     2521 tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag tgacattttt
     2581 ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc aacgttttcc
     2641 agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt gcccggcagt
     2701 gaaacctgtc caccgttaag cttgccctgc agttgagtaa tttccagcaa acccgacttg
     2761 ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac gttactggcc
     2821 tgcaacaaaa tatcagccgt aaagccttgc agtccctgat aggccggttc atcaatacgc
     2881 gaagaaataa ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc ggcaccgttt
     2941 tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg cgggaattgc
     3001 agtctcagct gccattcagg tttctctgtc agcgtgacct gtgcttgccc gctcagcgta
     3061 ctgtcattgg cggtcaagct gatttggtta aatgaaaggc gtttatgtga ctcctgccac
     3121 tgggcctgga aactcccctg cccctgaata ccttgttttg gtaaatcggc accctgcaac
     3181 tgccagttaa tttgttcaat agcagccgtt aaatcatgcg gataatcaga agcatctacc
     3241 gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc ggagaactca
     3301 aatgaaccac gatgttgggg atcttgttcc atttgcaggc gaatattgcg gattgtcacc
     3361 tgctcgtcat cttcatgctg gaacaccagc acgctgtccg ccaccttaag actggttata
     3421 tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg cggtgcaacc
     3481 ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac tgcccctttt
     3541 agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc cacgtccaga
     3601 cgcatgttgt cggcgcgaac cagtggctgg ctcgcgccct gggcggtgag agacattcgc
     3661 ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc gagctgcaat
     3721 tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc attcggattc
     3781 accagcaaca ctaacgcaga taacccggcc accagcacga ccaggagtat catcagcgtc
     3841 gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc gattcggctg
     3901 gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta aggtcgcgcc
     3961 gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg acgcagcgcc
     4021 agcggcagct taccggagtt gtagaactcc agcacaatac agccagacca gcccggatcg
     4081 atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccagtgagga acgaccgtcc
     4141 agccatccca ccagatcggc tggcagcgtc accgactcca gcgtcaccgc cagcgccagc
     4201 tctcctgggt gaagataaaa tgcctcgctt tcatcgagta cgatctcatc gctcatcacg
     4261 cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa cgctgccgtg
     4321 tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc gccgttaata
     4381 cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca ggcttcaata
     4441 tctcggtcac acagacgcat ggcattttct cctttcgcgc atcactccct taacgccaat
     4501 tacgtcaagg gcataccagg ttatcactga acggtacaca atttatcaag cttattcaaa
     4561 gaactgactt attttcgctt tcaatatatc gatcgcaatg cggtttttcc cgccgcgcgg
     4621 cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca tcggacgcac
     4681 ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt taacgtcacg
     4741 cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg agaagttaag
     4801 ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga tgactttctt
     4861 cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt aaaccggcag
     4921 gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc tgtgatccat
     4981 cgcgctagga tggtcgtagt tggtcttaat gcgttcttcc atcgacagat ggctttgatc
     5041 tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt gctcacgtaa
     5101 ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc cagcgatacc
     5161 gataatgacg cactgatgag actgttcagt catatattta gcgacctgat taacctggat
     5221 gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca tccgcctgat
     5281 accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacgaagaa ttcccggtgg
     5341 caaattacgt tgatcagttt tatacaaggt aaaaaatgtt atacgcagtt gcgcaaatta
     5401 tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt ttgtactagc
     5461 ataaacacag aaacaaatac tggcgacctg gtcttgcgga taaagcggta atgagcaaac
     5521 aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc agtttaggtt
     5581 tagtctcgtt tatctttacc cttttctcgc ttgagctttc gcagtttggc acccaactcg
     5641 ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat gccgggcgca
     5701 tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc atcctgcttt
     5761 tttccaccag ctcgctgaac atgacctgga cgaccatcaa tattgttgaa gccgtggtcg
     5821 gggcagtgct gctacgtaaa ttgctgccgt ggtataaccc cttgcaaaat ctggctgact
     5881 ggctgcgtct ggcactcggc agcgccattg ttccgcctct gttggggggt gttctggttg
     5941 tcctgctgac gcccggagac gatcctctca gggcattttt gatatgggta ctgtcagaat
     6001 ccatcggcgc tctggcactg gtgccgctgg gattgttatt taagccacac tatctgctgc
     6061 gccatcgcaa cccacggtta ctttttgagt cactgctcac gttagccatc acgctgacgt
     6121 taagctggct ttctatgttg tacctgccgt ggccttttac tttcattatt gtgctgttga
     6181 tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt gatcttcctt accacggtga
     6241 tgatggtgtc gctgatgatg gccgcggatc cctccctgct tgctacgccg cgtacatacc
     6301 tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct gctgcccgcc aatatcatga
     6361 cgatggtgat gtatgccttt cgtgcggaac gcaaacacat ttccgaaagc gaaccccgtt
     6421 ttcggaacgc catggaatat tccgctatcg gtatggcgtt agtgggcacc gagggacaat
     6481 ggctgcaaac caacaaagcg ctctgccagt ttctcgggta cagtcaggaa gagctgcgcg
     6541 gactcacctt tcagcaactg acctggccgg aggatctcaa taaagatctc caacaggttg
     6601 aaaagctgat aagcggtgaa ataaacacct attcaatgga aaaacgctac tacaaccgca
     6661 atggcgatgt tgtctgggcg ttgcttgccg tctcactggt gcgccacacg gatggcacgc
     6721 cgctctattt tatcgctcag attgaagaca ttaacgagct aaaacgcacc gaacaggtca
     6781 atcagcaact gatggagcgc atcacgctgg ctaacgaagc gggcgggatt ggcatctggg
     6841 agtgggagtt gaagccgaat atttttagct gggataagcg gatgttcgag ctgtatgaaa
     6901 ttcctccgca tatcaaaccg aactggcagg tgtggtacga gtgtgtattg ccggaagatc
     6961 gccaacacgc cgaaaaagtg attcgtgatt cgttgcaatc acgctcgccc tttaaactgg
     7021 aatttcgcat taccgtaaaa gacggtattc gccatatccg cgccctcgcc aaccgggtac
     7081 tgaataaaga aggcgaagtc gaacgtctcc tcggcatcaa tatggatatg accgaagtga
     7141 aacagcttaa cgaggcattg tttcaggaaa aagagcgcct gcacattacg cttgattcca
     7201 tcggtgaagc cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagtgg
     7261 cggagaagat gagcggctgg acgcaggaag aagcgttagg tgttccgctc ctgacggtgt
     7321 tgcatattac ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct
     7381 cacgttccgc gatcgagcaa gatgtggtgt tgcactgtcg gagcggcggc agttacgacg
     7441 tgcattacag tattacgccg ttaagtactc tggacggcag caatattggt tcggttctgg
     7501 tgattcagga cgttaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc
     7561 atgatgcact gacgcatctc gccaaccgcg ccagttttga gaaacaactg cgtatcctgc
     7621 tgcaaacggt aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct
     7681 ttaaagcggt gaatgacagc gccgggcatg cggcgggtga cgctttactg cgcgaactgg
     7741 cgtcgttgat gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg
     7801 aatttggtct actgttgcca gactgtaata tcgaaagtgc gcgttttatc gctacacgca
     7861 ttatcagtgc cgtgaatgat tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg
     7921 ccagtgccgg gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc
     7981 aggctgatat cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggtttacg
     8041 aaccacagca agctgccgca catagcgaac gggcggcgat gtcgcttgat gaacagtggc
     8101 ggatgattaa agagaatcag ttgatgatga tggcccacgg tgtcgcttcg ccgcggatcc
     8161 cggaagcgcg taatttgtgg ctgatttcac ttaagctctg gagttgcgaa ggcgagatta
     8221 ttgatgaaca aacatttcgt cgtagcttca gcgatccggc gcttagccat gctcttgacc
     8281 gccgggtatt ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt aaaggcataa
     8341 gcatcgccct ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgatctgc
     8401 ttgagcagct ggaaaatagc cctctaccac cacggttatt acatctgatt attccggctg
     8461 aagcgatttt agatcacgca gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag
     8521 tgctcagcca ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg aatatggcag
     8581 attacctgct acttgatggt gagttatgcg ccaacgtgca gggtaatttg atggatgaga
     8641 tgctgattac gattattcag gggcacgctc agcgactcgg gatgaaaacc atcgccgggc
     8701 cagtcgtttt acccttagtg atggatacgc tttctggcat cggcgtcgat ctgatttatg
     8761 gtgaggtgat tgccgatgcc caaccgctgg atttgctggt gaatagtagt tatttcgcga
     8821 ttaactgagg cgtgcttccc catcgcctga tgcgacgcta acgcgtctta tcatgcctac
     8881 aaatcgctca ttccccaggc cggataaggc gctcgcaccg catccggcga ccaacgtcat
     8941 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
     9001 ggcttccagc gctcggcata acggcggatt tgcgccggtg tcattcccgg aaatcgctgt
     9061 ttaatcagat aatcatccgg caggaaaaca tctttcgcct gccagccacg caaagcaaaa
     9121 taattcgccg tccagctccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
     9181 acatcgcccg gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc
     9241 agcgcctctg cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtctgctgct
     9301 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
     9361 taaagctgtg ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct
     9421 aaaatcgccc gcacgccctg ctcaaaagca tcaacactgc tgggtaaacg caatccgggc
     9481 cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
     9541 agcaggcggc tcattttcgc cagacactca gcggcaacgg gttctaaacc tacacttaaa
     9601 tttatgtgca gagtatggcg ggctatatcc ggaacagcag tcaccacgcc gcgatattcg
     9661 cccaccgcca gactacgggc ataataactg tccgcgaccg tttccacacc gctcaccgca
     9721 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcgactgcca gttcagggta
     9781 tacatcgcat ctccttattc atccgctttc agcataaacg ttattcagac gctacgcttt
     9841 gctttcatat tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac
     9901 cggcatgggg attaattcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
     9961 gcggtcatgc gtgacggtaa accgcaatta ctgaaaatgg aaaacgacag cacgctgctg
    10021 ccttcaatgc tttgtgcgcc aacgcgtgaa gcggtaagcg aatggttgta ccgccatcat
    10081 gatgttccgg cagacgacga tgaaacgcag gcgctgttgc gtcgggcgat tcgttataac
    10141 cgcgaagaag atatcgatgt tacggcgaaa agcgtgcagt tcggactttc ctcactggca
    10201 cagtacattg atgatccgga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
    10261 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
    10321 ttgcacattc gccagcaggc gcaggcacag ctgccagaaa cgattactca ggcggtgatc
    10381 ggtcgcccta tcaacttcca ggggcttggc ggcgatgaag caaacaccca ggcgcagggg
    10441 attctggaac gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gtacgagccg
    10501 gtagcggcag ggctggatta cgaagcgact ttgcaggaag aaaaacgggt gctggtggtg
    10561 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
    10621 cgtctcgatc gtgaagccag cctgctgggt cacagtggtt gtcgtattgg cggtaacgat
    10681 ctggatatcg ccctggcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
    10741 gaaaaaggca tcgccctgcc gatcctgccg tggtggaatg cggttgccat caacgacgta
    10801 cctgcgcaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
    10861 gatgcccgcg aaccggaaaa agtggccctg ctacagaaag tctggcgtca gcgtttaagt
    10921 tatcgcctgg tgcgtagcgc agaagagagc aaaattgcgc tttcaagcgt agcggaaacc
    10981 cgcgcctcac tgccgtttat cagcgatgaa ctggctacgc tgattagcca gcaagggctg
    11041 gaaagcgccc tcagccagcc actgacgcgg attctggaac aggtgcaact ggccctggat
    11101 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gaagcgcccg ttctccgctg
    11161 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
    11221 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tgtttcgtta attgaaaaat
    11281 ggctcagcag cggcttaaat ctgcctgcgc ctgagccagt ttttctgccg cctgacgtaa
    11341 cggttccatc aacggcatgg tccgtgctgg agcatgaagg cggaatcgct gcaactctgt
    11401 cgcgccgaca gtcaacgccg attccagccg atgtcgtttg gcggccactt tcgccattac
    11461 cgcttgtctg tctgcaggcg ttaccgcttc gttcggcctg aacacaaaac ggcgttcaca
    11521 gctcgctttc cagtcgatga ccgcctgggt cagatgatca ccaaaccctt taacttgctt
    11581 aaccccacga cgggtaacat ccgctgctgt ttcaatacca aaggaccgta acgccgcttt
    11641 acgcgcaggg ccaacaccgg gaatagaggc aacatcaata aaaaatccct ccagaaactt
    11701 ctgcttctgc cgttcccttg cggtgtcgtg aagtgctgcc agagcacgtt tttcttcctc
    11761 cggtaacccg agcatttcgt ccttcatttt ttcgagcatc gtccgtttgg cgataaaacc
    11821 ttccagcccg cccaactgtt ggatctggct gactaaatgg tcatagtcca ttttcgcgcg
    11881 attaaatgcc tcacggcgtt gctggatttc tgctttataa gctttgcttg tcagactgcc
    11941 aataatccag atagccgcca gcacaggaac caaaataata taacgcggtt ctgcctgaag
    12001 gccgcaaagc aacagcgata acgctgacag tgcgatctca agcagaatga tgtattcgcg
    12061 ccgtaacagg cctaaaggaa gcggccggcc agtcggttgg aaatgatcgg gtaatggcaa
    12121 ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc cagactttcg ccagcacaaa
    12181 atcaccgctg gtggtaatga cctcttcgcc gagatcaata aaatagataa cgccttgatt
    12241 atccagcgca caccacgggc agtcagtcaa atgaccgggg taaacatgca ttgccgaaac
    12301 ggtacatttc tttaactgtt ggcgtagaga atccagtgct gatacccacg cttttgccgt
    12361 cggacgcccg gttgccacgc cactttccgt gaatgcctgc tgaaacatgg cttcaacatc
    12421 actcggtaac atcgatagcg gaatcgatcg tggcggcggt tttaaaccac gccgctgatt
    12481 atctgactcg taggcataac ggaaatgagc aatatccgtc tccagcgcat tccccgcatc
    12541 agagataagc ggaacaccgg aataaggatg ccgcccacca aacaagacgt gaaaaatcag
    12601 caacgcaagg ccaaaattat cgtgattttc tgtgcgttca aagccgacaa atgacgacat
    12661 ggtttgtagc tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagtgtgcc
    12721 attggcgtta atctgaaagg agtcgctatc aatcaacacc actttgctgt cgcgacctac
    12781 cataaaactg ttctggttta cgtcacccac gacgtgcccg tgctcgtgaa ccgtagcaaa
    12841 agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat gagggtaact
    12901 ctggcgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg
    12961 catcataaaa ccgataactt ttccgcctcg tccaccgtga agcgttgcct gcggccaggc
    13021 gacataattc aacaactgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt
    13081 taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta tcggcaaact cctcgatatc
    13141 ataaaccgca ccttcaccgc ctttgcccag ttcacggcct ggcgtaacgc actcaccagt
    13201 agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt tttatcatca
    13261 tcggtacgct cgtttaccgc cgggctcgac aaaaactgct ttaacaattc aggtaataaa
    13321 tcgagttgtt cctgcgttgc tgccgccagt ccattgaaaa acggggtaaa gaaaggcaca
    13381 tgcggagaat tatccagcat atttaacgcc agtcgctgaa taccatccgt aaatgctgca
    13441 actttatgcg cacgctcagt gctggtgaaa gtttccagtc tggaaacagc atcttcatcg
    13501 ctaatgaaat gcgtcatatt ggcatattca cccaccatcg gcgtgagcgg cagttccaga
    13561 ccacgaccaa aatccaccac tacaccgccg tcgccaattt gcataatcaa tgtgccgtta
    13621 gccgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc gaactgctaa tgctttggct
    13681 tctgcctcgg caaaaagtcg ttgacgaatg gtcagtacca tatttgtggc gaggacatca
    13741 ttaagcccca attcaccgtc ctgtactttt tgcaccataa acgccatcgc ttcattgacg
    13801 gcgagcttcg cgccttctcc tccctgcgaa acgctacctg cgccatcagc aaagaacatc
    13861 accagtaatg gctgctggtc atttagccag gcaacctgca tctggcaggc atcctgacaa
    13921 ggtaaatcag cgctaatgtg cgacgttcca acagcggatg cataaaccag acgccagctc
    13981 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcagg
    14041 aaaccgaacg cagtgaactg gataaccagc taaacagttc gcggaattgt aatccctgca
    14101 gagacaaagg ctgacggacg ctgatttggg ccagcgtctt catatccgcc ccctgaacac
    14161 cgatggaaaa gaaggcaaac ttcttatctt cttccccctg aaacactttg ttggcggcag
    14221 cctgccactc atcggttgga gcgccatcgg tgatcaggaa aatccacggg cgataatacg
    14281 aaataccatt tgcacgatac tcacgtttcc gctcctctac catatccagg gctttggtaa
    14341 tggcggcacc cattggtgta tcgccctggg caaacaggat gggcgggaaa aaattagcgg
    14401 cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatacca agttccactc
    14461 tttttaaggc cagcgggtcg gcaagcagct catcgcgaaa ggtaaccaat ccggcgttaa
    14521 gttcattaat gggtctaccg ttcatcgaac cagaaacatc cagcagcaaa atgcaggggc
    14581 aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
    14641 gaaatccttt cttagagaat tattgaggtc cggtatcata ccgattgcaa taatgaaata
    14701 agagcaaacg aagaaacttt cagaattagg cccggcggcg cagaaaattt tgctattcgc
    14761 ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa tttcaggagt
    14821 gagggttagg gagaggtttc cccctccccc tggtgttctt agtaagcctg gaagctaatc
    14881 actaagagta tcaccagtat gatgacgtgc ttcatcataa ccctttcctt atttaaaagc
    14941 cctcttctcc gggagagggt tccccgtttt agcgtcccgc tgaaatcgtc ggcttacctc
    15001 ctttcgccct gaatgcagtc tatcgctgac ttgcggataa aatagtttca agtgtgatta
    15061 ttgcgtaacg actcgcaaag aatatttgct ggcaggatcg cagactgcaa agcctgcaga
    15121 ttgacaatct tatcgtgaag gcatactttc aggagtgagg gtagagcggg gtttcccccg
    15181 ccctggtagt cttagtaagc ggggaagctt atgactaaga gcaccacgat gatgagtagc
    15241 ttcatcatga ccctttcctt atttaaggcc ccttcctcgg gaggggcttt cccgtttcag
    15301 cgtcccgctg aaatcttcgg cttacctcct ttcgtcctgt atgcagtcta tcgctgactt
    15361 gcggataaaa tagtttcaag tgtgattatt gcgtaacgcc tcgcaaagaa tatttgctgg
    15421 caggatcgca gactgcaaag cctgcggatt gacaatctga ccgtgaaggc atactttcag
    15481 gagtgagggt tgagcggggt ttcccccgcc ctggtagtct tagtaagccg ggaagcttat
    15541 gactaagagc accacgatga tgatccactt catcatgacc ctttccttat ttatggcccc
    15601 ttcctcggga ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt
    15661 cgccatgcaa gcagtctatc gctaacgcgt agataaaata gtttcctgtg ttattactgg
    15721 atgcgtgctc gcaaatgtgc ccgccattca gacgattccc gatagtgttt cataattcct
    15781 ccatttttct cccttattgg ctggctacac tagtatcatt ccgcgaaacg tttcaggaag
    15841 agaaactctt aacgatgaaa ggcagttata aatcccgttg ggtaatcgta atcgtggtgg
    15901 ttatcgccgc catcgccgca ttctggttct ggcaaggccg caatgactcc cagagtgcag
    15961 ccccaggggc gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt ggtatgcgtt
    16021 ccggcccatt agccccggtt caggcggcga ccgccgtaga acaggcagtt ccgcgttacc
    16081 tcaccgggct tggcaccatt accgctgcta acaccgttac ggtgcgcagc cgcgtagatg
    16141 gtcagttgat ggcgttacat ttccaggaag gccagcaggt caaagcaggc gatttactgg
    16201 cagaaattga ccccagccag ttcaaagttg cattagcaca agcccagggc caactggcaa
    16261 aagataaagc cacgcttgcc aacgcccgcc gtgacctggc gcgttatcaa caactggtaa
    16321 aaaccaatct cgtatctcgt caggaactgg atgcccaaca agcgctggtc agtgaaaccg
    16381 aaggcaccat taaggctgat gaagcaagcg tcgccagcgc acagctgcaa ctcgactgga
    16441 gccgcatcac cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat gttggtaacc
    16501 aaatctccag tggtgatacc accggaattg tggtgatcac ccagacgcat cctatcgatt
    16561 tgctctttac cctgccggaa agcgatatcg ctaccgttgt gcaggcgcaa aaagccggaa
    16621 aaccgctggt ggtagaagcc tgggatcgca ccaactcgaa gaaattaagt gaaggcacgc
    16681 tgttaagtct cgataaccaa atcgatgcca ctaccggtac gattaaagtg aaagcacgct
    16741 ttaataatca ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc atgttagtcg
    16801 acaccgaaca aaacgccgta gtgatcccaa cagccgccct gcaaatgggc aatgaaggcc
    16861 attttgtctg ggtgctgaat agcgaaaaca aggtcagcaa acatctggtg acgccgggca
    16921 ttcaggacag tcagaaagtg gtgatccgcg caggtatttc tgcgggcgat cgcgtggtga
    16981 cagacggcat tgatcgcctg accgaagggg cgaaagtgga agtggtggaa gcccagagcg
    17041 ccaccactcc ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct
    17101 gatgcaggtg ttacccccga gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc
    17161 tgtggccacc acgctgctga tggtggcgat cttactcgcc gggattatcg gttatcgcgc
    17221 cctgcccgtt tcggcgctgc cggaagtgga ctatccgacc attcaggtgg tcacgctcta
    17281 cccaggtgcg agcccggatg tcatgacctc tgccgttacc gcgccgctcg aacgccagtt
    17341 cgggcagatg tctggtctga aacagatgtc gtcacaaagt tccggcggtg cgtcagttat
    17401 cactttgcag ttccagctaa cattaccgct ggatgtcgcc gagcaggaag tacaggccgc
    17461 gattaacgct gcgaccaact tgttgccgag cgatctgcct aacccgccgg tttacagcaa
    17521 agtgaacccg gcagatccgc cgatcatgac gctcgccgtc acctcaacag ccatgccgat
    17581 gacgcaagtg gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg
    17641 cgtcggcctg gtgacgcttt ccggcggcca gcgtccggct gttcgcgtca aacttaacgc
    17701 tcaggcgatt gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc
    17761 taacgttaac tcggcaaaag gtagcctcga cggcccttcc cgcgcggtta cgctttccgc
    17821 gaatgaccaa atgcaatctg cggaagaata tcgccagttg attatcgcct accagaacgg
    17881 cgcgccgatt cgtctgggcg atgtcgcaac tgtagagcaa ggtgcagaaa acagctggct
    17941 cggcgcatgg gcgaacaaag aacaggccat tgtgatgaat gttcagcgcc agcccggtgc
    18001 taacattatc tccaccgccg acagcatccg gcagatgctg ccacagctca ccgagagttt
    18061 gccgaaatcg gtgaaggtga cggtactttc tgaccgtacc accaatatcc gcgcatctgt
    18121 cgatgatact cagtttgaat tgatgatggc tatcgcgctg gtagtcatga ttatctacct
    18181 gtttttgcgc aatattccgg cgaccatcat tcccggtgtt gccgtgccgc tgtcgttaat
    18241 cggcactttt gcggttatgg tgtttctcga tttttcaatc aataacctga cactgatggc
    18301 gttaactatc gccaccggat tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc
    18361 ccgctatatc gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat
    18421 cggctttacc attatctcgc tgaccttctc actgattgcg gtgttgatcc cactgctgtt
    18481 tatgggcgat atcgtcgggc gactgttccg cgaatttgct attaccctgg cggtagcgat
    18541 tttgatctca gcggtggtgt cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag
    18601 ccaggagtcg ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag
    18661 gataatcgcc gcctatggtc gtggactggc gaaggtgctg aatcatccgt ggctgacctt
    18721 aagcgtggcg ctcagcacgc tgctgcttag cgttctctta tgggtgttca ttccgaaagg
    18781 tttcttcccg gtacaggaca acggcattat tcagggcact ttgcaggcac cgcaatccag
    18841 ctcctttgcc aatatggccc agcgacaacg ccaggtcgcg gacgtgattt tgcaggatcc
    18901 ggcagtgcaa agcctgacct catttgttgg cgttgatggc actaacccgt cgctgaatag
    18961 tgcacgttta caaatcaacc tcaaaccgct ggatgaacgt gatgaccgag tgcaaaaagt
    19021 cattgcccgt ctgcaaacgg cggtggataa agtgccgggc gtcgatctct tcctgcaacc
    19081 aacgcaggat ctgactattg atactcaggt cagccgcacc cagtaccagt ttaccttgca
    19141 ggccacctca ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa aactccagca
    19201 actgccacag ctttctgatg tctccagcga ctggcaggac aaagggctgg tggcgtatgt
    19261 caatgttgat cgcgacagcg ccagccgtct ggggatcagc atggcggatg tcgataacgc
    19321 cctgtacaac gcgtttggtc agcgactgat ttccactatt tatactcagg ctaaccagta
    19381 tcgcgtagtg ctggagcaca acaccgaaat taccccgggc ctcgcggcgc tggataccat
    19441 tcgcctgacc agtagcgacg gcggcgtggt gccgttaagc tcaattgcca aagttgaaca
    19501 gcgttttgcg ccgctttcca tcaaccatct ggatcagttc ccggtaacga ccatctcctt
    19561 taacgtgccg gataactatt cgctgggcga tgcggtgcag gcgattatgg acaccgaaaa
    19621 gacgctgaat ctgccggtgg atatcaccac gcaatttcag ggcagcaccc tcgccttcca
    19681 gtcggcgctg ggcagcactg tctggctgat tgtcgcggcg gtggtggcga tgtatatcgt
    19741 gctcggcatt ctgtacgaga gctttattca cccgatcacc attctctcga cgctacccac
    19801 cgcaggggtc ggcgcgctgc tggcattaat gattgccggt agcgaactgg atgtgattgc
    19861 gattatcggc attattttgc tgatcggtat tgtgaagaag aacgccatca tgatgatcga
    19921 cttcgcgctg gctgctgagc gcgagcaagg catgtcgccg cgcgatgcaa tctaccaggc
    19981 ttgtctgttg cgttttcgtc cgatcctgat gaccaccctg gcggctctgc ttggcgcgct
    20041 gccgctgatg ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag gtatcggcat
    20101 ggtcggcggt ctgattgtca gccaggtgct gacgctgttc accacgccgg tgatttatct
    20161 gctgttcgac cgcctggcat tgtggaccaa aagtcgcttt gctcgccatg aagaggaggc
    20221 gtaagtgaag ttttttgccc tcttcattta ccgcccggtg gcgacgattt tactgtcggt
    20281 cgccattacc ctgtgcggca tactgggctt ccgtatgctg ccggtcgccc cgctgccgca
    20341 ggtcgatttt ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac cagaaacaat
    20401 ggcgtcttcc gttgccacgc cgctggagcg ctcacttggg cgcattgccg gagtcagtga
    20461 aatgacctcc agcagttcgc tcggcagcac gcgtattatt ttgcagtttg attttgaccg
    20521 ggatatcaac ggcgcggcgc gcgatgtgca ggcggcgatc aacgctgcac aaagtttgct
    20581 gcccagcggg atgcccagcc gtccgaccta tcgcaaagct aacccgtcgg atgcgccaat
    20641 tatgatcctc acgctgacat ccgatactta ttcgcagggt gaactgtacg attttgcctc
    20701 gacgcagctg gctccgacga tttcgcaaat cgacggtgtc ggtgatgtcg atgttggcgg
    20761 cagctcgctg cccgccgtac gcgtcgggct gaatccgcag gcgctgttta atcagggcgt
    20821 ctcgctggac gacgtacgca ccgccatcag caatgccaac gtgcgtaaac cgcagggggc
    20881 gctggaagat ggcactcacc gctggcagat ccagaccaat gatgagctaa aaaccgccgc
    20941 cgaatatcag ccgttgatta ttcattacaa caacggcggc gcggttcgtc tgggcgatgt
    21001 ggcgacggtg accgactcgg tgcaggatgt gcgcaacgcc gggatgacca acgccaaacc
    21061 ggctattttg ctgatgatcc gcaagctgcc ggaagccaat attatccaga cggtagacag
    21121 catccgggca aaattaccgg agttgcagga aaccattccg gcggcgattg atctgcaaat
    21181 tgctcaggat cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc aaacgctgat
    21241 tatctcggtg gcgctggtga ttctggtggt gtttttattc ctgcgctcgg gtcgcgccac
    21301 tattattccc gccgttgcgg tgccggtttc gctgattggt acgtttgcgg cgatgtacct
    21361 gtgcggattc agtctcaata acctttcgtt aatggcgctc accatcgcta ctggtttcgt
    21421 ggtggatgac gccatcgtgg tgctggaaaa cattgcccgt catctggaag cggggatgaa
    21481 accgctacag gccgcgctac aaggtactcg cgaagtcggc tttacggtgc tgtcgatgag
    21541 tctgtcactg gtggcggtgt tcctgccgct gctgttgatg ggcggattgc cgggccgact
    21601 gttacgcgaa tttgccgtga cgctttctgt cgccattgtt atatcgttgc tggtttctct
    21661 gacattaacg ccaatgatgt gtggctggat gctgaaagcc agcaagccgc gtgagcaaaa
    21721 gcgactgcgt ggttttggcc gcatgttggt agccctgcaa caaggctacg gcaagtcgct
    21781 gaaatgggtg ctcaatcata cccgtctggt gggtgtggtg ctgcttggca ccattgcgct
    21841 gaatatctgg ctgtatatct cgatcccgaa aaccttcttc ccggagcagg acaccggcgt
    21901 gctgatgggc gggatacagg cggatcagag catttcgttt caggcgatgc gcggcaaatt
    21961 gcaggatttc atgaaaatta tccgtgacga tccggcagtg gataatgtca ccggctttac
    22021 cggcggttca cgagttaaca gtgggatgat gtttatcacc ctcaagccac gcgacgaacg
    22081 cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgta aaactggcga aagaaccggg
    22141 ggcgaatctg ttcctgatgg cggtacagga tattcgcgtt ggtgggcgtc agtcgaacgc
    22201 cagctaccag tacacgttgt tatccgacga cctggcggca ctgcgcgaat gggagccgaa
    22261 aatccgcaaa aaactggcga cgttgccgga actggcggac gtgaactccg atcagcagga
    22321 taacggcgcg gagatgaatc tggtttacga ccgtgacacc atggcacggc tgggaatcga
    22381 cgtacaagcc gccaacagtc tgttaaataa cgccttcggt cagcggcaaa tctcgaccat
    22441 ttaccagccg atgaaccagt ataaagtggt gatggaagtg gatccgcgct atacccagga
    22501 catcagtgcg ctggaaaaaa tgttcgttat caataacgaa ggcaaagcga tcccgctgtc
    22561 gtatttcgct aaatggcaac cggcgaatgc cccactatcg gtgaatcatc agggattatc
    22621 ggcggcctcg accatttcgt tcaacctgcc gaccggaaaa tcgctctcgg acgccagtgc
    22681 ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg gcagttttgc
    22741 cggaacagcg caggtgttcc aggagacgat gaactcacag gtgatcctga ttatcgccgc
    22801 catcgccacg gtgtatatcg tgctgggtat cctttacgag agttatgtgc acccgctgac
    22861 gattctctcc accctgccct cggcgggtgt tggagcgctg ctggcgctgg agctgttcaa
    22921 tgccccgttc agcctaatcg ccctgatagg gatcatgcta ttaatcggca tcgtgaagaa
    22981 aaacgccatt atgatggtcg attttgcgct tgaagcccaa cggcacggta acctgacgcc
    23041 gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga tgactaccct
    23101 ggcggcgctg tttggcgcgc tgccgctggt gttgtcgggc ggcgacggct cggagctgcg
    23161 gcaacccctg gggatcacca ttgtcggcgg actggtaatg agccagctcc ttacgctgta
    23221 taccacgccg gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt cgcgtaaacc
    23281 taaacaaacg gtaaccgagt aaatgacaga tcttcccgac agcacccgtt ggcaattgtg
    23341 gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtaa acaccgccct
    23401 tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg tcattgtctc
    23461 ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg acaaagtcgg
    23521 cgtgcgcaat attttcttta ccgccatcgt gctgtttact ctcggttcac tgttttgcgc
    23581 gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg ttggcggcgc
    23641 gatgatggtg ccggtcggca gattgacggt gatgaaaatc gtaccgcgcg agcaatatat
    23701 ggcggcgatg acctttgtca cgttacccgg tcaggtcggt ccgctgctcg gtccggcgct
    23761 cggcggtctg ctggtggagt acgcgtcgtg gcactggatc tttttgatca acattccggt
    23821 ggggattatc ggtgcgatcg ccacattgat gttaatgccg aactacacca tgcagacgcg
    23881 gcgctttgat ctttccggct ttttattgct ggcggttggc atggcggtat taaccctggc
    23941 gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc tggtcgcagt
    24001 tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaacc gcgccctgtt
    24061 cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc ctggcgggga gctttgccgg
    24121 acgtattggc agtggcatgt tgccctttat gacaccggtt ttcctgcaaa ttggcctcgg
    24181 tttctcgccg tttcatgccg gactgatgat gatcccgatg gtgcttggca gcatgggaat
    24241 gaagcgaatt gtggttcagg tggtgaatcg ctttggttat cgtcgggtac tggtagcgac
    24301 cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact accgctctgt tgggctggta
    24361 ctacgttttg ccgttcgtcc tgtttttaca aggaatggtc aactcaacgc gtttctcctc
    24421 catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg gaaacagcct
    24481 gctgtcgatg agtatcggcg tcaccatcgc cgggctgttg ctgggacttt ttggttcgca
    24541 gcatgtcagc gtcgacagcg gcaccacaca aaccgtcttt atgtacacct ggcttagcat
    24601 ggcgttgatc atcgcccttc cggcgttcat ctttgccaga gtgccgaacg atacgcatca
    24661 aaatgtagct atttcgcggc gaaaaaggag cgcgcaatga agttctggcg acccggtatt
    24721 accggcaaac tgtttctggc gattttcgcc acctgcattg tcttgctgat cagtatgcac
    24781 tgggcggtgc gtatcagttt tgagcgtggc tttattgatt acatcaagca tggtaatgaa
    24841 caacgattac aactgttaag tgatgcgctt ggcgagcagt atgcgcagca tggcaactgg
    24901 cgcttcctgc gcaacaatga tcgctttgtc tttcagatcc tgcgttcatt tgaacacgat
    24961 aattcggaag ataaacccgg cccgggtatg ccaccgcacg gctggcgtac ccagttctgg
    25021 gtggttgatc aaaacaacaa agtgctggtt ggtccgcgag cgccgattcc acctgacggt
    25081 acacggcgac ccattctggt caacggtgcg gaagttggcg cggtgatcgc ctcccccgtt
    25141 gagcggttaa cgcgcaatac tgatatcaat ttcgataaac aacagcggca aaccagctgg
    25201 ctgatcgtcg ccctggcaac gttactcgcg gcgctcgcca cctttctgct ggcgcgcggt
    25261 ttgctggcac cggtaaaacg acttgtcgat ggcacgcaca aactggcggc gggcgatttc
    25321 actacccgcg taacgcccac cagtgaagat gaactgggca aactggcgca agacttcaac
    25381 cagcttgcca gcacgctgga gaaaaaccag caaatgcggc gcgattttat ggccgatatt
    25441 tcacacgaac tgcgtacgcc attagcggtg ctgcgcggtg aactggaagc cattcaagat
    25501 ggcgtgcgca aattcacgcc ggagacggtg gcgtctttac aggcggaagt cggtacactg
    25561 actaaactgg ttgacgatct tcatcagttg tcgatgtctg atgaaggcgc tctcgcctat
    25621 caaaaagcac cggtagattt gatcccactg ctggaagtgg cgggcggcgc atttcgcgaa
    25681 cgatttgcca gccgtggcct gaaactgcag ttttccctgc cagacagtat taccgtattt
    25741 ggcgatcgcg accgtttaat gcagttattc aataacttac tcgaaaacag cctgcgctac
    25801 actgatagcg gcggcagcct gaaaatctct gccgagcagc acgacaaaac ggtgcgcctg
    25861 acctttgccg acagtgcgcc aggtgtcagt gacgagcagc tacaaaaatt atttgaacgt
    25921 ttttatcgca ccgaaggttc ccgcaaccgc gccagcggtg gttccgggct ggggctggcg
    25981 atttgcctga acattgttga agcacataat ggccgcatta ttgctgccca ttcgcctttt
    26041 ggcggggtaa gcattacagt agagttaccg ctggaacggg atttacagag agaagtatga
    26101 ccgagttacc aatcgacgaa aacacaccgc gtattttgat cgtggaagat gaaccgaagc
    26161 tggggcagtt gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg cttatcagcc
    26221 acggcgatca ggtactgccg tatgtgcgcc agacaccacc ggatctgatc ctgttagatc
    26281 tgatgctccc tggcaccgat ggcctgacgc tgtgccggga aattcgtcgt ttttctgaca
    26341 ttccgatcgt gatggtgacg gcaaaaatcg aagagatcga tcgcctgctg gggctggaga
    26401 ttggcgcaga tgattatatc tgtaagccgt acagcccacg ggaagtggta gcgcgcgtca
    26461 aaaccatttt gcgccgttgc aaaccgcagc gcgagttgca gcaacaggat gctgaaagcc
    26521 cgttgattat cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg cttgacctga
    26581 cgcctgcgga atttcgtctg ctgaaaacgc tctctcatga accaggaaaa gtgttctccc
    26641 gcgagcattt gctcaatcat ctttatgacg actaccgcgt agtaaccgac cgcaccatcg
    26701 acagccacat taaaaacctg cgccgcaagc tggaatctct cgacgccgaa cagtcattta
    26761 tccgcgccgt ttacggcgtt ggttaccgct gggaagccga cgcctgccgc atcgtttagt
    26821 tttagcgaca tgactttgtt agccggagat ggcgttcaga ccagatccgg caacattatc
    26881 ccacgcatgg tcagcaaact gacattatct ctccgccctg gcaggcatca gaaaatcgct
    26941 catactttaa tcggtaaaca gcacctttag atgctgtttt cgatacgcaa tttcaatcaa
    27001 ggagtcatta tggctggttg gtttgaactc agtaagagca gtgataatca gttccggttt
    27061 gtgctaaaag cgggcaatgg tgagactatc ctcaccagcg agctgtatac ctcaaaagcc
    27121 tctgcggaaa agggcatcgc gtcggtgcgt agcaacagcc cgcaagaaga acgctatgag
    27181 aaaaaaacgg caagtaacgg caaattctat ttcaatctga aagccgctaa tcatcaaatt
    27241 atcggctcca gccagatgta cgccaccgcg caatctcgtg aaaccggaat tgcctccgtt
    27301 aaagccaatg gcacaagcca gacggtgaaa gacaatacgt aattacgatg ccgggcgtgg
    27361 ggtgttggcg tccggcttta tatccaaata acgcttagtt attaataatt aagagtaacc
    27421 gccgccatta aaatagtggg cactgacgag cactcgttgt gctttctttg tttaccatcc
    27481 catacttatc ctcctgaaca tgttcatgtg ccaaaatatc ttctaatact ttcgtgacgc
    27541 tatccggttt atgacataca ctgatgtaag atttttactt acaggcgggc gtaaaatggc
    27601 cagaacgatg acggttgatc tcggcgatga gttgcgtgaa tttatcgagt ccttaattga
    27661 atccggcgac tatcgcaccc aaagcgaagt gatccgtgag tcactgcgat tacttcgaga
    27721 aaagcaagca gagtctcgcc tgcaagcgtt acgtgacatg ctcgcagaag ggttgagtag
    27781 tggtgaagct cagccgtggg aaaaggacgc gttcttacgg aaggtgaaag cagggataag
    27841 gaaataatgt gcgtattatt aaactcatgc caaaagccaa tgaggattta gaaggcatct
    27901 ggtattacag ctatcaccat ttcggcgaac cacaagccga cagatatgtg gaacatcttt
    27961 cagacgttct ccagattctg agtaacaata atataggtac gcccagacct gaattaggcg
    28021 aaggtatttt tgttcttcct tttgaacgcc acgtcattta tttcctgcaa tcaccagggg
    28081 aaattatcgt cattcgtatc cttaaccaaa accaggacgc caccagacat ttacactgga
    28141 gctaaacccg ccgattgcgc tacaatgccc gcccttaaag tgggggcact cccctaaccg
    28201 cttcatcagg tgaagcggat ctgacctgtc atcagaacga gagaattatg tttaaaccgg
    28261 aactcctttc cccggcggga acgctgaaaa atatgcgtta cgctttcgct tatggcgcag
    28321 atgctgttta tgcgggccag ccgcgttatt ccctgcgtgt gcgcaacaac gaattcaacc
    28381 acgaaaatct tcagctcggc atcaatgaag cccacgcgct ggggaaaaag ttttatgtcg
    28441 tggtcaacat tgcaccgcac aacgccaagc tgaaaacctt tatccgtgac ctgaaaccgg
    28501 tggtggaaat ggggccggat gcgctgatta tgtccgatcc agggctgatt atgctggtgc
    28561 gtgagcactt ccctgaaatg ccgatccacc tttcggtgca ggctaacgcc gtgaactggg
    28621 cgacggtgaa attctggcag caaatgggcc tgacccgcgt gatcctctct cgcgagctgt
    28681 cgctggaaga gattgaagag atacgcaatc aggtgccgga tatggagatc gagatcttcg
    28741 ttcacggcgc gctgtgcatg gcctactccg gtcgctgcct gctctctggc tatatcaaca
    28801 agcgcgaccc gaaccagggc acctgcacca acgcctgccg ctgggagtac aacgtccagg
    28861 aagggaaaga agatgatgtt ggcaacatcg tacacaagta cgagccgatt ccggtgcaaa
    28921 atgttgagcc gacgctgggt atcggcgcac caaccgacaa agtgtttatg atcgaagagg
    28981 cccagcgtcc gggcgagtat atgaccgcgt ttgaagatga gcacggcact tacatcatga
    29041 actcgaaaga tctgcgcgcc atcgcccatg tagaacgcct gaccaaaatg ggcgtgcatt
    29101 cgctgaaaat cgaaggtcgt accaaatctt tctactattg tgcacgcacc gcacaggttt
    29161 accgcaaagc tatcgatgac gccgctgcgg gaaaaccgtt cgataccagc ctgctggaaa
    29221 ctctggaagg tctggcgcat cgtggctata ccgaaggttt cctgcgtcgt catactcacg
    29281 acgattatca gaactacgaa tacggttatt cagtttctga ccgccagcag tttgttggtg
    29341 agtttaccgg tgagcgcaag ggggacctcg cggcggtagc ggtgaaaaat aaattctccg
    29401 ttggcgacag ccttgagctg atgacgccgc aaggcaacat taattttacc cttgagcaca
    29461 tggaaaacgc caaaggcgaa gctatgccga tagcaccagg cgatggttat actgtgtggc
    29521 tcccggtccc gcaggatctt gagctcaatt acgcgctgct gatgcgtaat ttctccgggg
    29581 aaaccacgcg taatccccac ggtaagtgat taatttcgat tatttttccc ggatggaaaa
    29641 ttcttagaaa ccgatcacat acagctgcat ttattaaggt tatcatccgt ttcgctgaaa
    29701 aacataaccc ataaaatgct agctgtacca ggaaccacct ccttagcctg tgtaatctcc
    29761 cttacacggg cttatttttt atgcataagc cctatccctg gtcaccgtct tccattgacc
    29821 acatcgatag aatctccctt catagcacga tgcctttcac gtaacggcat cgtgctcgca
    29881 caggttccgg ctaagcacaa ccagaacgcg catgtttgac gcttaccaaa aaatattctc
    29941 actctccaca tttgaatgtc agacgagcga cgccatgtaa tcctgcacct tctgtcttca
    30001 ggtcaactat ctgcattttt ttgccctgag taacacagaa atgggctgca tcatttttta
    30061 ctatattttc tgcaccagat attctgcccc tggctaaaga agcttcggct tcggtgtagt
    30121 attggttatc gagtttacgc tgaatattac ttttatatgc aagaccaaat ttaccgatac
    30181 ttgtctcatc attatgcaca gcacaaccag acataataaa aatactaatt aatgatatag
    30241 cagctatctt tttcatctca ccttccccca ttaaatacca a
//
LOCUS       DAZGTR010000049        30086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGTR010000049 DAZGTR010000000
VERSION     DAZGTR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.049"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..710
                     /locus_tag="ACHLCY_002395"
     CDS             <1..710
                     /locus_tag="ACHLCY_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0369642.1"
                     /translation="LDDGRLSYSLEQSLDDDNNHNSSLNASYRSPYGTFSAGYSYGND
                     SSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDANGASGVRIQNYPGIATDPFGYAVV
                     PYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDR
                     NGKPLPFGALASNDETGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFST
                     PDSEPTTSVLQGTAQCH"
     gene            721..1812
                     /gene="yraK"
                     /locus_tag="ACHLCY_002396"
     CDS             721..1812
                     /gene="yraK"
                     /locus_tag="ACHLCY_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0369643.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTNRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(1855..2715)
                     /gene="rsmI"
                     /locus_tag="ACHLCY_002397"
     CDS             complement(1855..2715)
                     /gene="rsmI"
                     /locus_tag="ACHLCY_002397"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP0369644.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2779..4815
                     /gene="lpoA"
                     /locus_tag="ACHLCY_002398"
     CDS             2779..4815
                     /gene="lpoA"
                     /locus_tag="ACHLCY_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP0369645.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4773..5168
                     /gene="yraN"
                     /locus_tag="ACHLCY_002399"
     CDS             4773..5168
                     /gene="yraN"
                     /locus_tag="ACHLCY_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP0369646.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5188..5778
                     /gene="diaA"
                     /locus_tag="ACHLCY_002400"
     CDS             5188..5778
                     /gene="diaA"
                     /locus_tag="ACHLCY_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP0369647.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            5788..6363
                     /gene="dolP"
                     /locus_tag="ACHLCY_002401"
     CDS             5788..6363
                     /gene="dolP"
                     /locus_tag="ACHLCY_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0369648.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6477..7517)
                     /gene="yraQ"
                     /locus_tag="ACHLCY_002402"
     CDS             complement(6477..7517)
                     /gene="yraQ"
                     /locus_tag="ACHLCY_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0369649.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7590..8225)
                     /gene="yraR"
                     /locus_tag="ACHLCY_002403"
     CDS             complement(7590..8225)
                     /gene="yraR"
                     /locus_tag="ACHLCY_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0369650.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            8353..8871
                     /gene="yhbO"
                     /locus_tag="ACHLCY_002404"
     CDS             8353..8871
                     /gene="yhbO"
                     /locus_tag="ACHLCY_002404"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP0369651.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNRGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8851..9294)
                     /gene="yhbP"
                     /locus_tag="ACHLCY_002405"
     CDS             complement(8851..9294)
                     /gene="yhbP"
                     /locus_tag="ACHLCY_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP0369652.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            9345..9647
                     /gene="yhbQ"
                     /locus_tag="ACHLCY_002406"
     CDS             9345..9647
                     /gene="yhbQ"
                     /locus_tag="ACHLCY_002406"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP0369653.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9634..10137)
                     /gene="yhbS"
                     /locus_tag="ACHLCY_002407"
     CDS             complement(9634..10137)
                     /gene="yhbS"
                     /locus_tag="ACHLCY_002407"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0369654.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10131..10655)
                     /gene="ubiT"
                     /locus_tag="ACHLCY_002408"
     CDS             complement(10131..10655)
                     /gene="ubiT"
                     /locus_tag="ACHLCY_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP0369655.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10864..11859
                     /gene="ubiU"
                     /locus_tag="ACHLCY_002409"
     CDS             10864..11859
                     /gene="ubiU"
                     /locus_tag="ACHLCY_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP0369656.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11868..12746
                     /gene="ubiV"
                     /locus_tag="ACHLCY_002410"
     CDS             11868..12746
                     /gene="ubiV"
                     /locus_tag="ACHLCY_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP0369657.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12952..13959
                     /gene="yhbW"
                     /locus_tag="ACHLCY_002411"
     CDS             12952..13959
                     /gene="yhbW"
                     /locus_tag="ACHLCY_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFP0369658.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14077..15321)
                     /gene="mtr"
                     /locus_tag="ACHLCY_002412"
     CDS             complement(14077..15321)
                     /gene="mtr"
                     /locus_tag="ACHLCY_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP0369659.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15475..17364)
                     /gene="deaD"
                     /locus_tag="ACHLCY_002413"
     CDS             complement(15475..17364)
                     /gene="deaD"
                     /locus_tag="ACHLCY_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP0369660.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEEQDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17357..17437)
                     /gene="yrbN"
                     /locus_tag="ACHLCY_002414"
     CDS             complement(17357..17437)
                     /gene="yrbN"
                     /locus_tag="ACHLCY_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP0369661.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17544..18428)
                     /gene="nlpI"
                     /locus_tag="ACHLCY_002415"
     CDS             complement(17544..18428)
                     /gene="nlpI"
                     /locus_tag="ACHLCY_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP0369662.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18537..20672)
                     /gene="pnp"
                     /locus_tag="ACHLCY_002416"
     CDS             complement(18537..20672)
                     /gene="pnp"
                     /locus_tag="ACHLCY_002416"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP0369663.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20919..21188)
                     /gene="rpsO"
                     /locus_tag="ACHLCY_002417"
     CDS             complement(20919..21188)
                     /gene="rpsO"
                     /locus_tag="ACHLCY_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP0369664.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21337..22281)
                     /gene="truB"
                     /locus_tag="ACHLCY_002418"
     CDS             complement(21337..22281)
                     /gene="truB"
                     /locus_tag="ACHLCY_002418"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP0369665.1"
                     /translation="MSRPRRRGRDINGVLLLDKSQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22281..22682)
                     /gene="rbfA"
                     /locus_tag="ACHLCY_002419"
     CDS             complement(22281..22682)
                     /gene="rbfA"
                     /locus_tag="ACHLCY_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP0369666.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22846..25518)
                     /gene="infB"
                     /locus_tag="ACHLCY_002420"
     CDS             complement(22846..25518)
                     /gene="infB"
                     /locus_tag="ACHLCY_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP0369667.1"
                     /translation="MTDVTIKTLAAERQTSVEGLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25543..27030)
                     /gene="nusA"
                     /locus_tag="ACHLCY_002421"
     CDS             complement(25543..27030)
                     /gene="nusA"
                     /locus_tag="ACHLCY_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP0369668.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27058..27510)
                     /gene="rimP"
                     /locus_tag="ACHLCY_002422"
     CDS             complement(27058..27510)
                     /gene="rimP"
                     /locus_tag="ACHLCY_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP0369669.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27717..27793)
                     /locus_tag="ACHLCY_002423"
     tRNA            complement(27717..27793)
                     /locus_tag="ACHLCY_002423"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27757..27759),aa:Met,seq:cat)
     gene            28141..29484
                     /gene="argG"
                     /locus_tag="ACHLCY_002424"
     CDS             28141..29484
                     /gene="argG"
                     /locus_tag="ACHLCY_002424"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP0369670.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(29492..>30086)
                     /locus_tag="ACHLCY_002425"
     CDS             complement(29492..>30086)
                     /locus_tag="ACHLCY_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0369671.1"
                     /translation="PHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDD
                     QDACYDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQ
                     AYHVPMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHY
                     KGDSRVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
BASE COUNT         7203 a         8241 c         7555 g         7087 t
ORIGIN      
        1 tgctggatga tggtcgcctg agctatagcc tggaacaaag tctggatgac gataacaacc
       61 ataacagtag cctgaacgcc agttaccgtt caccttatgg caccttcagt gccggataca
      121 gctacggtaa tgacagcagc caatacaatt acggcgttac cggcggcgtg gttatccatc
      181 ctcatggcgt gacgctctcg caatatctgg gcaacgcttt tgcgcttatc gatgctaatg
      241 gggcttctgg cgtgaggata caaaactatc cggggattgc tactgatccc tttggctatg
      301 cagtggttcc ttatctcacg acttaccagg aaaaccgtct ctcggtagat actacgcagc
      361 tgcccgataa cgtcgatctt gagcaaacaa cacagtttgt ggtgcccaac agaggtgcaa
      421 tggtagcggc gcgtttcaac gccaatatcg gttatcgcgt acttgttaca gtcagcgatc
      481 gcaacggtaa accgttgccc tttggcgctc ttgccagcaa cgatgagacg gggcaacaaa
      541 gtatcgtcga tgagggcggc atactatatc tctctgggat atcgagtaaa tcacaaagct
      601 ggactgtacg ctggggaaat caggcagatc aacaatgtca gtttgctttt agtacaccgg
      661 attcagaacc aacaacctct gtattacaag gcacggcgca gtgccattaa ggataaaaaa
      721 atgaaaagag cgcctcttat aacaggactt ttgttgatat ccacatcctg cgcttatgcc
      781 tcctcaggag ggtgtggagc tgacagcact agcggtgcga caaattacag cagtgtggtt
      841 gatgatgtta cggtgaacca gacagataac gtgacaggac gggagtttac ctctgccacg
      901 ctaagtagca ctaactggca atacgcctgt acctgctctg cgggtaaggc agttaaactt
      961 gtctatatgg tcagccctgt acttaccacc actggacatc aagcaggata ttacaaactc
     1021 aatgatagtc tggatattaa aaccacatta caggcgaatg acattccagg actcgtgact
     1081 gaccagaccg tttctgttaa cacccgattc acacagataa aaagcaacac cgtatattct
     1141 gctgcaaccc aaacgggtgt ttgccagggt gacacgtctc gttatggacc cgttaatatt
     1201 ggtgcaaata ccacctttac cctgtatgtc accaagccat ttctcggctc gatgaccatt
     1261 ccgaaaacgg atattgccgt cattaaaggc gcatgggtcg atggaatggg aagcccgtct
     1321 acaggtgact tccatgattt agtcaagtta tcgattcagg gaaatctcac cgccccacag
     1381 tcgtgcaaaa ttaatcaggg cgatgttatt aaggttaatt ttggattcat caatggtcag
     1441 aagtttacca accgcaatgc catgccagac ggttttactc cagtagactt tgatatcact
     1501 tatgactgtg gtgatacttc aaagattaaa aactcgttgc aaatgcgcat cgacggtaca
     1561 actggggtag tagaccagta caacctggtc gccagacgaa gaagttcaga caatgcgccc
     1621 gatgtcggta ttcgtattga aaatctcggc ggcggagttg caaatattcc ttttcagaac
     1681 ggtatccttc ccgttgatcc ttccgggcat ggcaccatca acatgcgcgc ctggccagtt
     1741 aatctggtcg gtggtgagct ggaaacagga aaatttcagg gcaccgccac cattaccgtc
     1801 atcgtgcggt aaaagaagac gttgcgtttt gattaacgca acgcttcggc actcttaccc
     1861 ctgctgctcc agcgcatact tatacagcgc atttttcttc acgccgtgaa tttctgccgc
     1921 cagcgccgcc gcttttttca acggcagttc tgcctgtagc agcgccagcg tgcgcagggc
     1981 atcggcgggt aagtcttctt cctgtgcttt atgaccttcg ataatcagca ccatttcgcc
     2041 tttgcgacgg ttttcatctt cttttaccca cgccagcagc tcgccaacgg gcgcgccgtg
     2101 aatggtttcc caggttttgg tcagctcacg cgccagaacc acgtagcggg attcgcctaa
     2161 taccgcaacg atatcttcca ggctatctaa cagacggtgg gtggattcat aaaaaatcag
     2221 cgtgcgcggc tccgcttcaa tggcttttag cgcatcacgg cggccttttg atttggcagg
     2281 taaaaagcct tcgtaacaga aacggtcaga gggtaaaccc gcggcgctta acgcagtgat
     2341 agcagcacac ggcccgggta gcggcaccac gcaaatcccc gcttcacggc aggtacgcac
     2401 cagatggtag ccaggatcgt taattagcgg cgttccggca tcggaaacca gcgcaatgtt
     2461 ctgcccttct tgcagcttcg ccagcagcgt ttcggctttt tgttgttcgt tatggtcgtg
     2521 cagcgcaaac aaccgggcat taatcccaaa atgttgcagc aataaaccgg tgtgacgagt
     2581 atcctcggcg gcaatcagat caacggcctg taatacctct aacgcacgct gggtgatatc
     2641 cgccagattg ccgattggcg tcggtacaat gtaaagctgg ccctgagaat tatccgccga
     2701 ttggtgttgt ttcattgtgt cgtccgtatt gccgatttaa tattgagcat tgcgtaaaaa
     2761 aatatcactg gatacattat ggtaccctca acattttctc gtttgaaagc cgcgcgttgt
     2821 ctgcctgttg ttctggcagc cctgattttc gccggttgtg gcacccatac tcccgatcag
     2881 tccactgctt atatgcaggg cacggcgcag gctgattctg ccttttatct tcagcagatg
     2941 cagcaaagct ctgatgatac caggatcaac tggcaattac tcgccattcg tgcactggtg
     3001 aaagaaggta aaaccgggca ggcggttgag ttgtttaacc aactaccgca agaactgaac
     3061 gattctcagc gtcgcgagaa aacactgctg gcggtagaga ttaaactggc gcagaaagat
     3121 tttgctggcg cgcaaaactt gctggcgaaa atcacacctg ccgatttaga acaaaaccag
     3181 caagcgcgtt actggcaggc aaaaatcgat gccagccagg ggcgtccttc cattgattta
     3241 ctgcgcgcgt taattgctca ggaaccgctg ctcggcgcga aagaaaaaca gcagaatatt
     3301 gatgccacct ggcaggcgct ctcctccatg actcaggaac aggcgaatac gctggtgatc
     3361 aacgccgacg aaaatattct gcaaggctgg ctggatctgc agcgcgtctg gtttgataac
     3421 cgtaacgatc ccgacatgat gaaagccggg atcgccgact ggcaaaaacg ttatccgaac
     3481 aatccgggcg cgaaaatgct gccaacgcag ttggttaacg taaaagcgtt taaaccagcc
     3541 tcgaccaaca aaatcgccct gctgttgccg ctgaatggcc aggcagcggt atttggtcgc
     3601 actattcagc aaggctttga agcggcgaaa aatatcggca ctcagccagt ggcggctcag
     3661 gtagctgccg cacctgccgc agacgtagct gaacaacctc agccgcaaac cgtggatggc
     3721 gttgccagcc cggcacaagc ctcggttagc gatctgaccg gtgaacagcc tgcggcacag
     3781 ccagcgcctg taagcacccc ggcagcaacc accacagcgg taagcgcgcc agcgaatcca
     3841 tccgcagaac tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag
     3901 gttcagcagg atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag
     3961 ttgcttaaga gcaacacccc gctgaacgtg ctggcactga accagccgga gaatatcgaa
     4021 aaccgcgtca atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg
     4081 cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg
     4141 ggcgatcgcg tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt
     4201 ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt
     4261 atcgctttaa cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc
     4321 aacaatccaa cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt
     4381 gtcgatgcgg tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc
     4441 gccatgcgta acggtagcca gagcggtgca acgctgtacg ccagctcccg cagtgcgcaa
     4501 gggaccgctg gcccggattt ccgactggag atggaaggct tgcagtacag cgaaatcccg
     4561 atgctggcag gcggtaatct accgttaatg cagcaggcac tcagcgcggt gaataacgat
     4621 tattcactgg ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc
     4681 tcacaaatgc gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca
     4741 aacccggatt gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta
     4801 gtccccgcca gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct
     4861 ggctggaagg caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggcgaga
     4921 tcgatctgat aatgcgtgaa ggccggacca ccgtttttgt cgaggtacgc tatcgccgct
     4981 ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac
     5041 agactgcccg cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt
     5101 tcgatgtggt agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc
     5161 actcataatt aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa
     5221 agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc
     5281 atgacgctgg ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact
     5341 tccgctgcca atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg
     5401 cccagcttac ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac
     5461 gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat
     5521 gtattgttag ccatttccac ccgtggcaac agccgcgata ttgtaaaagc ggtagaagct
     5581 gccgttacgc gtgatatgac tattgtggca ttgaccggct atgacggcgg cgaacttgca
     5641 ggtttgttag ggccacaaga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt
     5701 caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc
     5761 cctcaccagg atgtttaagg agaatacatg aaggcattat cgccaatcgc agtccttatt
     5821 tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt
     5881 accaaagccg caactgaccc acgcagtgtc ggcacccagg tggacgatgg taccctggaa
     5941 gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta agaaagaagc ccgtattaat
     6001 gtcacggcgt atcagggcaa agtgctgctg gttgggcagt caccaaacgc tgaactttcg
     6061 gctcgcgcca aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt
     6121 cgtcagggcc agccgattgg tctgggcgaa gcgtctaacg atacgtggat caccaccaaa
     6181 gtgcgttcgc agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc
     6241 gaaaacggtg aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca
     6301 gatattgcca gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa
     6361 tagcagcccc ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat
     6421 gcaaaccgta ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag
     6481 aacaacagcg ccagcccgcc gacaatcaca ccagacactg ccaccatcgc ccctgtcagc
     6541 cataaggctt tcgctgggaa cgctttgcgc agcataatca gtgacggcaa actcaccgcc
     6601 gggagcgtca tcaacaatgc cagcgccgga gcagttccca tacctgccag catcatcgtt
     6661 tgtacaatcg gaatttctgc tgccgtggga atgacaaaca agcatcctgc taccgccatc
     6721 gccatcaccc acatcaggct gttatcgaca gcaccatcgg catgggggaa taaccagacg
     6781 cgagcggcac ccaacaccag tactgcaagg atgtaaaccg ggatcgtact ccagaaaagc
     6841 gtccatagcg ccctgcccca gcggctaaaa aaaccgccct gtgcttccgg tatgtcaatt
     6901 tcgaccggtg cctgcgtttg cggtgtttca cgcacccatt tttgcaccag cgtcgcaatc
     6961 agcaacacca tcaccagccc ggccaccaga cgaatcgccg caaaacccca gccgaggaca
     7021 aaccccataa acaccagcgt cgccgggttt aacaccggat tgcccatcca gaatgccagc
     7081 gcaccgccca tcgacacctg ttggcgacgc attcccgccg cgaccggagc cgcacagcag
     7141 gtacacatca tgcccggcag cgaaaacagc gttcccagca gcgtgccgcg aaagcgcgat
     7201 tgcccaagcg tacgcaacaa ccagtcacgc gggatcagca cctgaatcaa cgaaccgagg
     7261 atcaccccca gcactgccgc tttccatacc gcgaggaaat agatcatcgc gtaatccaac
     7321 gccgcctgcc acggattagc atccgcctgc gcaaggatag atttaccgat actgtgggtt
     7381 tcggcagcag taaacgcttt gccgtagtaa ggttgccatt tcacatacca gagaccggca
     7441 atgacaacga gaaagaaaag cgcgggtttc caccactgaa tgggcgttgc cgcctgagat
     7501 gaagactgac cagtcatagc attccccagg aataagttat gataattgag cgcgtgaata
     7561 ttacgctcac tatcaattct tgggaataat tattcagctc ttttgcgtaa ttctgaagag
     7621 cttaaaatcg tcacaccttc atgcccgggc cgcatcgcct ctgccagcat gacccgcgcc
     7681 acgtctctcg catcaatgga tttccagtta ccgggtaata aacggaacag cggcgcaaaa
     7741 agcgtttcgt tcatccgctg tttgctacga tcgcccagta acatcgacgg gcgagcaatg
     7801 gtcaatttcg gccagttctg ggcgattaat gcttcttcca tctccccttt gacgcggtta
     7861 tagaaaaacg gcgagtgggc attggcaccc atagcactga ccaccagcat atgctgcgcg
     7921 cccagtcgcc gcccggttaa tgcggtatcc actaccagcg tgtaatcggc atgaataaac
     7981 gcctctttgc tccccgcttc tcgccgcgtg gtgccgagac aacaaaacac aatgtcgatg
     8041 ggatcggtga cctgcgccag cgcgtcagtc agttgcggat catggggatt aaacaccccg
     8101 ggcatatcgc ccaacggacg tcgcgtcggc gcggctatgg cgttaacttt cggttcgtta
     8161 atcaacatcc gcagcaggtg accgcccacc agccccgttg cgcctgtaat cagtacctga
     8221 ctcatcttcg ctcctttaca gaattgtctg ccttgcgctc cacggctcat gcaccaggct
     8281 taatagacca tgaggtaatt atccccgatt gtgggaaatt cgcctcatcc aatgcaacaa
     8341 cgcggaggaa gcatgagtaa gaaaattgcc gttttaatca ctgatgaatt tgaagattca
     8401 gaatttactt cacccgcaga cgagttccgt aaagccggac acgaagtgat taccattgaa
     8461 aaacaagcgg gtaaaacggt gaaaggcaaa aaaggagaag ccagcgtgac catcgataaa
     8521 tccatcgatg aagtgacgcc agcggagttt gatgccctgc tgctaccggg cggccattca
     8581 ccggattatc tgcgtggcga caaccgtttt gtcaccttta cccgtgattt tgtgaataga
     8641 ggcaaacctg ttttcgccat ctgtcacggt ccgcagctgc tgatcagcgc cgatgtgatt
     8701 cgggggcgca aactgaccgc ggttaaaccg atcattattg atgttaaaaa cgcgggcgcg
     8761 gaattttacg atcaggaagt cgtggtcgat aaagatcagc tggttaccag ccggacaccg
     8821 gacgatctgc cagcgtttaa ccgcgaggcg ttacgcctgc tcggtgcctg agccgcgtag
     8881 ccaaatcatt tttttaccaa agcccagcgt gttgtcggtg aatttgattt catcgagccg
     8941 gatttcccac accggggccg acagcattct ggcaaccgga aagcgacgat tgtacgcctt
     9001 gcgcgcgagg tcgctttcct caccttccag cctgcggatc tcacctttaa actgcacgcc
     9061 gcgaattaac gctaccgttt tcggctgacc gtttaccgtt ccggcaaccg cagcctgcgg
     9121 cccactcatc tgcgcgtggc gcgttttttc ttccgtcaaa atgtagaagg cgactttctg
     9181 cgcatcaaac agataaaaag cattagcgca ccagagttcc ccttcctgct gcacacacca
     9241 ggtgacaaca tgttgttttg ccaaccaacg gctgatggtg atgagtgttt ccattgctgt
     9301 tctcttttat actgtgggcc tgaccttaac atgcgacgca gacgatgaca ccctggtttc
     9361 tttacttgat ccgtaccgcc gacaataagc tttataccgg gatcaccacg gatgtcgaac
     9421 gccgctatca gcagcaccaa agcggcaaag gggcgaaagc actgcgcggg aaaggagaac
     9481 tgacgctggc gttttctgcg ccagtgggcg atcgttcgct ggcgttacgg gcggaatatc
     9541 gcgttaagca actgaccaaa cggcaaaaag agcgtctggt agcggaaggc gcagggtttg
     9601 cagagctgtt aagcagtctg caaaccccgg agattaaaag cgattgaaat gctcgtgata
     9661 ctcaaccaga ccagttacgc cattcaacgc gtcatccgct aaacgatgta cctggaaggc
     9721 gctttcagtg cctggccagc ggcaacgcag gtcgtgatgt gccgccagtt caaagccaaa
     9781 acggctgtac agcgccggat cgcccagcgt caccactgcg gcatagccga actcattaag
     9841 cgaatcgagt ccttcataga ccagttggcg tgccagcccc tgtccacggt atttttcatc
     9901 aaccgccaac ggtgccatgc cgacccattg caggtcttcg ccctgcacat caaccggact
     9961 aaatgccaca tagccaatga cctgaccttc gtcatctgtc gccaccagcc ccagcgtcag
    10021 aaagccatct tcacgcagat cgtgaaccag cttcgcttcc gcatcacttt cgaatgagcg
    10081 acgtagcagg gcatcaatac ccggcgcatc aatgggaatt tctactcgaa ttagcatggt
    10141 tcacctaccg atgtctgttt ggtttcaggc gcggttttca ttcccgcctc aacaaaatcc
    10201 gccagttgca gcagcatcat gcgtaaggct ttcggcattt gctccagttc aatggcgtcc
    10261 atcaggtttt tcacatacag ccccagctcc gtatcgcctt caatcaccag ccgacgctgg
    10321 aagaagagcg tatccggatc ttgtttacgc gccgcaatca tcagcagatc gctggcgtcg
    10381 gcactaaaac tcacatcagc ttgcgcgttc tggctgacca ccagtttgcc attcaccacc
    10441 gaggtaaacc attgcaggtc aatatcacgc acatgaatac ttaaccagcg gccttcaaga
    10501 aactccagct cgccatcatc cagcgcctga cggaattgcc agcttaagac ctgctcaaga
    10561 acctggcgtt ttagcgcaaa tggcgtcagt tttaccggta cactcaacag agatggcccc
    10621 aaatgcacaa tacgggaacg cagtttatcc aacacgagtt ttactccctg tttcaacaat
    10681 catcctattt tgccatatca gaaaaataac atagcggtat aaatcaacaa ttccatatga
    10741 aattgctgct accaccaata caactttaac tgccttaaat caaaaattgt cgcagcaagg
    10801 ttaactaaaa tcccagttcg ttaacatttt tgcgttttga tagcgcaacc ttcaggaaaa
    10861 attatggagc tgctctgccc tgccggaaat ctcccggcgc ttaaggcggc catcgaaaac
    10921 ggcgcagatg ctgtttatat cgggctaaaa gatgatacca atgcccgtca cttcgccggc
    10981 cttaacttta ccgagaaaaa attgcaggaa gcggtgagtt ttgtccatca acatcgccgc
    11041 aaacttcaca tcgcgattaa cacttttgcg catccggacg gttacgcccg ttggcagcgt
    11101 gccgtggata tggcggcgca gctgggtgcc gacgcgctga tcctcgccga cctcgccatg
    11161 ctagagtacg ccgccgagcg ttacccgcat attgagcgtc atgtgtcagt gcaggcttcg
    11221 gcgaccaatg aagaggcgat taacttttat catcgccatt ttgacgttgc tcgcgtggtg
    11281 ctgccgcgcg tgttgtcgat tcatcaggtg aaacaactgg cacgggtcac acctgtacca
    11341 ctggaagtct ttgctttcgg cagcctgtgc attatgtcgg aaggtcgttg ctatctgtcg
    11401 tcgtatctga cgggtgagtc gcccaacacc gtaggcgcgt gttctccggc ccgtttcgtg
    11461 cgctggcaac aaacgccgca ggggctggaa tcccgcctga acgaagtgct gatcgaccgt
    11521 tatcaggacg gcgaaaacgc aggttatccg acgctatgta aagggcgtta tctggtggac
    11581 ggcgagcgct atcacgcgct ggaagaacca accagtctca ataccctgga actgctgccg
    11641 gagttaatgg cggcgaatat tgcttcggtg aaaattgaag gccgccagcg tagcccggcg
    11701 tatgtcagcc aggtggcgaa agtctggcgt caggctatcg accgttgtaa ggccgatccg
    11761 caaaacttca taccgcaaag cgcgtggatg gagacgctcg ggtcgatgtc cgaaggcacg
    11821 cagaccaccc ttggcgcgta tcaccgtaaa tggcagtgag aagagcaatg aaatattcct
    11881 tagggccagt gctgtggtac tggccaaaag agacgctgga agatttttat cagcaggccg
    11941 ccaccagcag cgccgacgtg atttatcttg gtgaagcggt atgcagcaag cgccgggcaa
    12001 ccaaagttgg cgactggctg gagatggcaa aatcgctcgc cggaagtggc aagcagattg
    12061 tgctctccac gctggcgctg gtgcaggcat cctctgaact gggcgaactg aaacgctatg
    12121 ttgagaacgg tgagtttctg attgaagcca gcgatctcgg cgtggtgaat atgtgcgctg
    12181 aacgcaaact gccgttcgtc gccgggcacg cgctgaactg ctacaacgcg gtgacgctga
    12241 aaatattgct caaacagggc atgatgcgct ggtgtatgcc ggtggagctt tcccgcgact
    12301 ggctggtgaa tctgcttaat cagtgcgatg agctgggcat tcgcaaccag tttgaagtgg
    12361 aagtcctgag ctacggtcat ctaccgctgg cctactccgc ccgctgcttt accgcgcgtt
    12421 cggaagaccg cccgaaagat gagtgtgaaa cctgctgcat taagtatccg aacgggcgca
    12481 acgtgctgtc gcaggaaaac caacaagtgt ttgtactcaa tggcattcag accatgagcg
    12541 gctacgttta caacctcggt aacgagctgg catccatgca ggggctggtt gatgtggtcc
    12601 gcctgtcacc gcagggtact gacactttcg cgatgctcga cgccttccgc gctaatgaaa
    12661 atggcgctgc gccactaccg ttgacggcaa acagcgactg taacggctac tggcggcggc
    12721 tggccgggct ggagctgcaa gcctaagtaa atatgctcgt catacttcaa gttgcatgtg
    12781 ctgcatctgc attcgttcac cccagtcact tacttatgta agctcctggg gattcactct
    12841 cttgtcgcct tcctgcaact cgaattattt agagcacagc tcactttgtt aacaacttta
    12901 actactcttt aatgcagtat taaagattaa tcggtaacaa agtgagctgt tatgactgat
    12961 aaaaccattg cgttttcgct actcgatctg gcccccattc ccgaaggttc ttcagcgcga
    13021 gaagcattct cccactctct cgatctcgcc cgtctggctg aaaagcgcgg ctatcatcgc
    13081 tactggctgg cagaacacca caatatgact ggcattgcca gtgctgccac gtcggtattg
    13141 attggctatc tggcggcgaa taccaccacg ctgcatctgg ggtctggcgg cgtgatgttg
    13201 cctaaccact caccgttggt cattgccgaa cagttcggca cgctcaatac actctatccg
    13261 gggcgaatcg atttggggct gggtcgtgcg ccaggtagtg accaacggac aatgatggcg
    13321 ctgcgtcgcc atatgagcgg cgatattgat aatttccccc gcgatgtcgc ggagctggtg
    13381 gactggtttg acgcccgcga tcccaatccg catgtgcgcc cggtaccagg ctatggcgag
    13441 aaaatccccg tgtggttgtt aggctccagc ctttacagcg cgcaactggc ggcgcagctt
    13501 ggtctgccgt tcgcgtttgc ctcacacttc gcgccggata tgttgttcca ggcgctgcat
    13561 ctttatcgca gcaacttcaa accgtcggca cgactggaaa aaccatacgc gatggtgtgc
    13621 atcaatatta tcgccgccga tagcaaccgc gacgctgagt ttctgtttac ctcaatgcag
    13681 caagcctttg tgaagttgcg ccgtggcgaa accgggcaac tgccgccacc gattcaaaat
    13741 atggatcagt tctggtcgcc gtccgaacag tatggtgtgc agcaggcgct gagtatgtcg
    13801 ttggtaggtg ataaagcgaa agtgcgtcat ggcttgcagt cgatcctgcg cgaaaccgac
    13861 gccgatgaga ttatggtcaa cgggcagatt ttcgaccacc aggcgcggct gcattcgttt
    13921 gagctggcga tggatgttaa ggaagagttg ttgggatagt gtgtcttaac gcgggaagcc
    13981 ttatccgagc tggcaacgct gtcctacgta gacctgataa gcgaagcgca tcaggcattg
    14041 tgtaggcagc agaaatgtcg gataaggcac cgctgattac tgatacaccg gcagtaaatt
    14101 aaagctcgat aaaatatgga ccagtgcgtt gccaacgcca aacaccagaa tcagcgtaat
    14161 catcggcttg ccgccccaga cgcggaattt cgggctgcca aagcgtttac gcgatgcgcg
    14221 ggctaacagc gccggaacaa ttgccgccca gatggtagct gctaaaccag cataaccaat
    14281 ggcgtacagg aatccgttcg ggaacagtag accacccaca acaggcgggg caaaggtcag
    14341 caatgccgtt ttcaagcgcc ccagggctga gtcatcgaaa ccaaacagat ctgccagata
    14401 gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct accgcaaagt ttgagaacac
    14461 gaccagcaga aggtccaggc tacggctgtt cagcacgccg cttaatgcct gtaccagcac
    14521 atcaatatta ccgcccttct ctgcaatacc gataaactcc ggacgcggga tgttacccat
    14581 cgtcgccagc aaccagatgg tatacagcgc cagtgccatc agcgtaccgt acaccaggca
    14641 tttcacgatg gttttcggat ctttgccgta atacttcatc aggcttggca cgttaccgtg
    14701 ataaccaaac gacgccaggc agaacggcaa tgtcatcagc agatacggcg cataagatgc
    14761 attgctttct gcaacgttga acaatgtcgc aggctgcaca tgccccaaca ggctgccaaa
    14821 ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc gccgtcatgc ggctgacggc
    14881 cttagtactc aaccacacca caaacgctac cagcagtgca aaaccaaaac ccgccgcccg
    14941 tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc agaattgaac cactggcaga
    15001 aatataggca taggtcagga tatagagcac aaaggcaatg gaaataccgt tgaccacgtt
    15061 ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa ctcgaaccga ttcggtaatt
    15121 caggttagct tccagaatca tcaagccgga atgcagcata cagaaccagg taaagatcag
    15181 cgccgccatt gaccagaaaa accacgcccc ggacatgacc actggcagag aaaacatccc
    15241 tgcgccaata atggtgccgc cgataatcac cacgccgcca agcagcgacg gtgacgtttg
    15301 ggtggtggtt agtgttgcca tgagggcttc tctccagtga aaaatagtgc gactgcgttg
    15361 ttatgcattg cactgtacca gtacacgagt acaaaagaca gaaaaaaagc cccgatggta
    15421 aaaatcgggg ctgtatatat tattttacag attgtgttcg ctgttcagcg atgattacgc
    15481 atcaccaccg aaacgacgac gaccggtaga gtcatcacga cgcggagcgc ggccttcacg
    15541 acgttcgccg ctaaaacgac gaccatcacc acggccacct tcacggcgtt caccgctgaa
    15601 gttacgaccg ccttcacgac gttcgccacc gaaaccacga ccaccgccac gacgctcacc
    15661 gccagtatgc ggctgcgcat cgcccagcaa ctgcatattc atcggcttgt tcagaatgcg
    15721 agtacgcgta aagtgttgca gtacttcacc cggcatacct ttcggcagtt cgatagtgga
    15781 gtgagaagca aacagcttaa tgttaccgat gtaacggctg ctgatgtcgc cttcgttagc
    15841 aatcgcgcca acgatatgac gaacttcaac accatcatcg cggcccactt caatgcggta
    15901 aagctgcatg tcaccaacat cacgacgttc acgacgcgga cgatcttcac ggtcgccacg
    15961 tgggccacgg tcgttacgat cgcgcggacc acggtcatca cggtcacgga attcacgttt
    16021 cggacgcatc ggcgcatctg gcggaacaat cagagtacgt tcaccctgtg ccattttcag
    16081 cagtgccgca gccagagttt cgagatcctg ctcttcacct tctgcagtcg gctgaatttt
    16141 gctcagcagt gcgcggtatt gatccagatc gctgctttcc agctgctgct gtactttagc
    16201 ggcgaatttt tccagacggc gtttgcccag cagttctgcg ttcggcagtt ctacttccgg
    16261 aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga cgacgctcgc ggttctcaac
    16321 gaacagcagc gcgcggccag cacgacccgc acgaccggta cgaccgatac ggtgaacgta
    16381 agactcagaa tccatcggga tatcgtagtt aactaccagg ctgatacgct caacgtccag
    16441 gccacgggct gcaacgtcgg tcgcaatcag gatgtccaga cgaccatctt tcaggcgttc
    16501 cagtgtctgt tcacgcagcg cctggttcat gtcaccgttc agcgcggcgc tgttgtagcc
    16561 gttacgctca agagcttcag ccacttccag agtcgcgttt ttggtacgaa cgaagataat
    16621 cgccgcatca aaatcttccg cttccaggaa acgtaccagt gcttcgtttt tgcgcatacc
    16681 ccagacagtc cagtagctct ggctgatgtc aggacgggta gtcacgctgg actgaatgcg
    16741 cacttcctgc ggctctttca taaagcggcg ggtaatgcga cgaatcgctt ccggcatggt
    16801 tgcagagaac agagcggtct gatgaccttc cgggatctgc gccataatgg tttcaacgtc
    16861 ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc agaaccagac cgctcagttt
    16921 agagaggtcc agagtgccac gtttcaggtg gtccagcaga cggcccggag taccgacaac
    16981 gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca taacgctggc cgccgtatag
    17041 agcaaccaca tttacgccgc gcatgtgttt agagaaatcc gtcattgctt cagcaacctg
    17101 taccgccagt tcgcgggtcg gtgccagcac cagaatctgt ggtgctttca gctcaggatc
    17161 aagattctgc aacagaggta aagagaatgc tgcagttttt ccgctccccg tctgggccat
    17221 acccagaacg tcgcggccat tcagcagatg tggaatacac tctgcctgaa ttggagatgg
    17281 tttttcgtaa cccagatcgt taagggcttc aaggatagga gccttcaggc ccagatctgc
    17341 aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc aaaattaatg gcggccagtc
    17401 tacataactc atcatgaaat tgatcagcaa ttttcattga aaagtgtgaa ccggctcaaa
    17461 gtaggtgtat taacgaacaa caacgccctc acccgttaag gtgatggcaa tcaaaaaaga
    17521 ttacgggctg atgtgtacgt cagctattgc tggtccgatt ctgccaggtc atcttggtcc
    17581 tggcccagga gcgataattc caacaatgcg tatcggtgct caacaaagtt atgaacgttg
    17641 ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca aatcccccag acttaggtag
    17701 tacttaccta aatagaagtt ggtttcactg agatgctcag cgagcgaggt gttatccgtt
    17761 gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa tgttgcccag gtagaactcg
    17821 acaatgttcc atccccactg ttccttatcc gatttttcga agtgctgttt caacacttct
    17881 ttagcctgct tctcatcgag cttctgctcg gcgagataaa gccacagact acggaaagga
    17941 tcattgggat cgtcttgata aaacgccagc agatcatctt gcgctaactt gtcacgaccg
    18001 ccgtaatata atgcgatccc gcgattcaag tgcgcgtagt tgtaagttgg atcaagctca
    18061 agtacagaat caaacgcttc ataggcagca tcaaaattgc ctgcctgcgt taaatatatg
    18121 cctaagtaat tgaatacttc aggcatatcc ggtcggattg ccagcgcttg cgaaaaatcg
    18181 ttacgcgcta atgccctcag accgagacta tcatacaaca ctccgcgctc atataaaagc
    18241 tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga tttgttccat acgtgccaga
    18301 atcacttcct gctgtaaagt cggttgcaat ggtaccgcga ggacttcact tttacgccag
    18361 gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa cgaaacacca gcgcaaaaaa
    18421 ggcttcattt cccactcccg aagaccacgg ttgaatgaac gtcctgttcc cggttgctaa
    18481 caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg agggcaaatg gcaaccttac
    18541 tcgccctgtt cagcagccgg agcttccggt gctgcagcag gttgagactg ctcagtcgct
    18601 tctttaatgc tcagacggat acggccctgg cgatcaactt ccagaacttt caccggtact
    18661 tcctgaccca tctgcaggta atcggtcact ttctcaacgc gtttgtcagc gatttgagag
    18721 atgtggacca gaccttcttt accgccgccg atggcaacaa atgcgccaaa gtcaacgata
    18781 cgggtcactt taccagtgta gacgcggccc acttcgattt ctgcagtgat ctcttcgata
    18841 cgacgaatag catgtttcgc tttctcgccg tcggtcgctg cgatcttcac agtaccgtca
    18901 tcttcgattt cgatggtagt gccagtttct tcggtcaggg cacggataac agagccgcct
    18961 ttaccgataa cgtctttgat cttgtccggg ttgatcttga tggtatggat acgcggtgcg
    19021 aactcagaga tatcgccacg cggcgcgttg atcgcctgtt ccattacgcc caggatatgc
    19081 agacgcgcac ctttagcctg gttcagcgca acctgcatga tctctttggt gataccttca
    19141 attttgatat ccatctgcaa tgcagagata ccctcgcggg aacccgcaac tttgaagtcc
    19201 atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca gtacaacgta gttgtcgcct
    19261 tctttcacca gacccattgc gatacccgca acggcagctt tgatcggcac acctgcgtcc
    19321 atcagcgcca gagacgcgcc gcacacggaa gccatagaag aggaaccgtt ggattcagtg
    19381 atttcagaca caacacgtac ggtgtacggg aatttgtcca tatccggcat gactgccagc
    19441 acgccgcgct tcgccagacg accgtgacca atttcacgac gcttcggaga accgaccatg
    19501 ccggtttcgc ctacggagta cggagggaag ttgtagtgga acaggaaggt atcggtacgt
    19561 tcgcccatca gttcatcaag aacctgcgcg tcacgagcag tacccagcgt tgcggtaacc
    19621 agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt gagtacgcgg cagcacgcca
    19681 gtacgcacat ccagaccacg gatcatatct ttttcacgac cgtcgatacg cggttcgcct
    19741 gccagtacgc ggctacgaac aacgtttttc tcgatagcgt gcagaatttc acccagttcg
    19801 ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga tggtttcaga tttgatgaca
    19861 tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt aagcatcgct caggcgagct
    19921 tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta ccggctccgg ctgccagtcc
    19981 caacgtggtt taccggcttc tttcaccagt tcattgatgt tctgaataac aacctgctgt
    20041 tgttcatgac cgaacactac tgcgcccagc atctggtctt cgctcagcag ttcagcttca
    20101 gattcaacca tcagaacagc ggcttcagta ccggcaacaa ccagatccag tttgctctct
    20161 ttcagctcgt cctgagtcgg gttcagtacg tactggtcat tgatgtaacc tacgcgagca
    20221 gcaccaatcg gaccattgaa cggaatacca gacagagaca gtgctgcgga agcaccaatc
    20281 atcgcgacga tatccgggtt aacttgcggg ttaacagaaa ccacggtggc gataacctga
    20341 acttcgttga cgaagccttc cgggaacagc gggcgaatcg ggcggtcaat cagacgcgcg
    20401 atcagggttt cgccttcgct tgggcggcct tcacgacgga agaagctacc cgggatacga
    20461 ccagcagcgt aggtacgctc ctgatagtta acggtcagtg ggaagaagtc ctgacctggt
    20521 ttggcttttt tctggccaac aacggtaaca aataccgcgg tgtcatccat gctaaccata
    20581 acagcggcag tagcctgacg agccatcatg ccggtttcca gagtcacggt gtgttggccg
    20641 tactggaatt tacgaacgat cggattaagc aatgtaatat cctttctctt tcttagacag
    20701 taccttacgg cactggtgtt aatacccgat cttctgcgca tcctcgcgac taatgacaac
    20761 cctaacccag ctctatgtgg gtaaagcctc tcattagccg cgcgaacctc tgcaacggaa
    20821 gatcattcat agcaacaata cattagtttc cagtgaattg ctgccgtcag cttgaaaaaa
    20881 ggggccactc aggccccctt ttctgaaact cgcaagaatt agcgacgcag acccaggcgc
    20941 tcgatgagct gggtgtaacg tgctacgtct ttacgtttca ggtagtcgag cagtttacga
    21001 cgctgagaaa ccatgcgcag cagaccacga cggctgtggt gatctttttt gtgctctgca
    21061 aagtggccct gcaggtggtt gatctgtgca gtcagcagtg ctacctgaac ttcggtagaa
    21121 ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt tagctgttgc ttcagtactt
    21181 agagacattt taaaactcca aagtatatag aatgaaagga cgccgatctc taattcagcg
    21241 atcccagtgt acgttacgca aagtgttaaa caatttacgc gacgttaagc ggcagtattc
    21301 tactcgtagc gacctgttat cgcaagacgg ttaacattac gccgggtatt caaccaccag
    21361 gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc ataccgataa atttgccgtt
    21421 ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca ccggatgtac gaactgggtt
    21481 accatttttg aagtaaacag aagacgttaa cggaagattc accaccgggt agtccgaagc
    21541 tggactgtcc attggcatca gtaatggatc aagtaactcc gcagccggaa tatcctgctg
    21601 ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc accatccgtt caaccggata
    21661 tttacttacc gccagacgac gcaggtaaat aacatgcgcg ccacaaccga gtttttcacc
    21721 caggtcatca atgatggtgc ggatataagt gccttttgag cagtgaattt ccagctccag
    21781 ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg gtaatcgggc gcgcttcacg
    21841 cggtacttca atgccctgac gcgcatattc gtacagtttt ttaccctgat atttcagtgc
    21901 tgaatacatc gaagggatct gttcgatatc gccacggaaa gtatccagcg ccgccgccag
    21961 ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt tgtccgtcgg catcagaagt
    22021 atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt ttgtcggagt ccagcagata
    22081 ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac atgccagttg ccagcgggtc
    22141 cagcgcaccg gtatgcccgg cacggttggc gttataaatc cgtttcactt tttgcagcgc
    22201 atcattgctg gacataccct gcgatttatc cagcaacaaa acgccgttaa tgtcgcgacc
    22261 gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt ccgggttaac acgacgttct
    22321 tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc gcatcccttc aaccagagag
    22381 ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc gcatcgcttt ccccagcagg
    22441 ctgcggatga aaccagaagc ttcttgcaac gctttgatgc ccgctttaac cgcgtcttca
    22501 tctttgtcgt tgaggaacgt cacatatact ttggcatacg ccaggtcgcg agacatttcg
    22561 acaccggaaa cggtggtcat catgcccagg cgaggatctt taatttcacg ctgcaggatg
    22621 agagcgatct ctttttgcat ttcctgcgct acgcgctgcg ggcgaccaaa ttctttcgcc
    22681 ataataaatt ctcctgacaa aaaaggggct gttagcccct ttttaaaatt aatttcaggt
    22741 ggaagggctg ttcacgttgg cctgataaga cgcgccagcg tcacatcagg caatccatgc
    22801 cggatgcggc ataaacgcct tatcccgcat ggaaccctaa aaaccttaag cgatggtacg
    22861 ttggatctcg atgatttcga atacttcgat cacatcgcca gtgcggacgt cgttgtagtt
    22921 cttaacgccg ataccacatt ccataccgtt acggacttcg ttaacgtcat ctttgaagcg
    22981 gcgcagggac tccagctcgc cttcgtagat aaccacgttg tcacgcagaa cgcggatcgg
    23041 gttgtgacgt ttaaccacac cttcggtaac catacagcct gcgatggcac caaatttcgg
    23101 cgatttgaac acgtcacgaa cttccgccag accgataatc tgctgtttca gttccggaga
    23161 cagcataccg ctcatcgccg ctttcacttc gtcaatcagg ttatagatga cggagtagta
    23221 acgcagatcc aggctttccg cttcaatcac tttacgtgca gaggcatcag cacgtacgtt
    23281 gaagccaacc aggatggcgt tggacgccgc agccagggtg gcgtcggttt cggtgatacc
    23341 acctacgcca gaaccgatga tcttcacttt aacttcgtca gtagacagtt tcagcaagga
    23401 gtcggagatc gcttccacag aaccctgtac gtctgccttc aggacgatgt tcacttcgtg
    23461 aacttcgcct tcggtcatgt tggcgaacat gttctcgagt ttagatttct gctgacgcgc
    23521 cagtttaact tcgcggaatt taccctgacg atacagtgca acttcacgcg ctttcttctc
    23581 gtcacgtaca acggtaactt catcacccgc agccggtacg ccggacaggc cgaggatttc
    23641 caccggaatg gacggacccg cttccagcac ttcctgaccc agttcgttac gcatcgcacg
    23701 aacacgaccg tattcgaagc cacacagaac tatatcgccc ttgtgcagag taccttcacg
    23761 taccagaacg gtagcaaccg gaccacgacc tttatcgagg aaggattcga taaccgcacc
    23821 gctcgccata cctttacgta ccgctttcag ctccagaact tccgcctgca gcaggatagc
    23881 gtccagcagt tcatcgatgc cggtacccgc tttcgcagat acgtgtacga actggctttc
    23941 accgccccac tcttccggca ggatgccata ctgggagagt tcgttcttaa cacgatccgg
    24001 atcggcttct ggtttatcaa tcttgttcac tgcaaccacc accggtacct gcgccgcttt
    24061 cgcgtgctgg attgcttcga tggtctgcgg catcacgccg tcgtcggcag caacaaccag
    24121 gactacgatg tccgttgcct gcgcaccacg tgcacgcatt gaagtaaacg cggcgtgccc
    24181 cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca acgtggtatg caccaatgtg
    24241 ctgggtaatg ccgcccgctt cgccagaggc aactttcgtt gaacgaatgt agtccagcag
    24301 agaggtttta ccgtggtcaa cgtgacccat gatggtcaca accggcgcgc gcggttcagc
    24361 cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc agctcgtttt cacgacgcag
    24421 gataactttg tggcccatct cttcagcaac cagctgtgcg gtttcctgat cgataacctg
    24481 gttgatggtt gccattgcgc ccagtttcat catcgctttg atgacctgag agcctttaac
    24541 cgccatcttg ttcgccagtt cgccaacggt gatggtttcg ccgatcacaa cgtcacggtt
    24601 aacggcctga gctggcttct ggaagccttg ctgcagggaa gaacctttac gttttccgcc
    24661 tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca gcttttgatt cagcgtgttt
    24721 gttgcctttc ttcggacgcg ctgctttcgc gttacgacca cggccacgac cgccttcgac
    24781 ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga gaagtagtga cgtgataatc
    24841 gctggaatct tcagtcggtt ccgcgttatc agtccatttg ttttcttccg ccatacgacg
    24901 tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta cgacgcgctt cttcttcagc
    24961 tttacgcttg agctctgcag cttcctgctc acggcgggct ttttcagcct gggcgttttt
    25021 agtcatatcg tcttgttgat tgctcacttt gtctttttcc gcagcttcac gtttcgcttg
    25081 ttcagcagct tcacgcttag cttgttctgc ggcctcacgt tcagcttttt gttgcgcctc
    25141 gcgtttagcc gattcttctg cctcacgacg ggcttgctct tccgcttcac gctgcgcttg
    25201 ctcttccgct gcaaggcgtt cagcctcttg cggatcgcgt ttcacaaagg tgcgtttctt
    25261 gcggacttcg atttgtaccg atttgctttt tccaccggta ccaggaatgt taagggtgct
    25321 gcgtgtttta cgttgcagcg tcaatttgtc cgggcctgaa tttttctgat tcaggtggtc
    25381 aatcaaagtc tgtttctctt gtgcagacac agagtcgtca gcagacttcc ggatacctgc
    25441 atcagcaaat tgctgtacca ggccttccac ggaggtctgt cgctctgcgg ccagcgtttt
    25501 aatcgttaca tctgtcatgc tgttccttcc tgctacagtt tattacgctt cgtcaccgaa
    25561 ccagcaaata ttacgggcag ccataatcag tgctccggct ttttcgtcgg tcaacccttc
    25621 gatatcagcc agatcatcaa tgccctgttc ggcgagatct tccagcgtac aaacgccacg
    25681 ggcggccagt ttgaatgcca aatcacgatc tatcccttca aggttcagca gatcgtcagc
    25741 cggtttgtta tcaccgaggc tttcttcctg ggcctgtgca atggtggcca gtgcattttt
    25801 agcacgctcg cgcagtgctt caacggtcgg ctcatcaagg ccttcgattt ccaacagctc
    25861 tttcatcggc acataggcca gttcttccag cgtcgagaag ccttcttcta ccagaacagt
    25921 cgcgaagtct tcgtcgatgt cgagatattt ggtgaaggtg tcgatcgctg cgtgcgcttc
    25981 cgcctgatgc ttagcctgca ggtcgtcaac ggtcatcacg ttgagttccc aaccgctcag
    26041 ctgcgaagcc agacgcacgt tctgaccgtt acggccaatc gcctgcgcca ggttaccggc
    26101 ttcaacggcg atatccatgg tgtgtttatc ttcatccacc acgatagaag caacgtctgc
    26161 cggtgccatt gcgttaatca caaactgcgc cgggttatca tcccacagga cgatatcgat
    26221 acgctcgccg cccagttcag tagaaaccgc ctgaacacgc gcgccacgca tacctacaca
    26281 ggcacctacc ggatcgatac gtttatcgtt ggttttcacc gcgattttcg cacgagaacc
    26341 cggatcgcga gccgctgctt taatttcaat cacttcttcg ccgatttctg gcacttcaat
    26401 acggaacagt tcgatcagca tttccggctt ggaacgagtg acgaacagtt gcgcgccacg
    26461 cgcttccggg cgaacggaat agagcacgcc acgaacgcgg tcgccagggc ggaagttttc
    26521 acgcggcagc atatcttcgc gcaggatcac ggcttcagca ttgttgccca gatccagaga
    26581 gatgttgtcg cggtttactt ttttcaccac gccggtgatg atttcacctt cgtgttcacg
    26641 gaactgatca accaccatcg cacgttcggc ttcacgcact ttctgcacga taacctgttt
    26701 tgccgtctgg gtagtgatac ggtcaaaggt aacagactca atctgatctt caacgtaatc
    26761 gcccaggttc aggctttcat cttcataacg tgcggcttca agggtgattt ccttggtcgg
    26821 ctgggtgact tcatcaacaa ctaaccagcg acggaaggtg tcaaaatcac cgcttttgcg
    26881 atcgatctgt acgcggacgt cgatctcttg ttcatatttt ttctttgttg ctgtcgccag
    26941 cgcgctttcc aatgcttcga aaatcttctc gcgaggtagc gccttttcat tggatacggc
    27001 ttcaactaca gccaaaattt ctttgttcat cgcgggcttt tcacctcatc cagactatta
    27061 aaagtgggga accaggttcg ccttctggat attactcagc gcgaacactt catctttacc
    27121 ttcgacggta actgtgatca tttcaccgtc taccgctttg ataacgccct gccatttacg
    27181 acggttttgt accgccatac ggagaaccag agtcacctct tctccgacaa aacgggcgta
    27241 gtgttcagcc gtgaacagtg ggcgatcgag acccggtgag gagacttcca ggttataagc
    27301 aacggtgatg ggatcttcaa catccagcac agcacttacc tggtggctca catcagcaca
    27361 atcatcaaca ttgatgccat cttcactatc aatatagatg cgcagtgtgg atgtgcgacc
    27421 gcgaataaat tcgatgccaa ccagttcaaa acccagggcc tcaactggcg cagtaatcat
    27481 ctctgttaat ttttgctcta atgtggacaa gcccaccccc aagacataaa aaaagggcct
    27541 aaagcccagt tattctgtag tcagataaca aaaaaccccg ataaatcggg gctttatata
    27601 actgaaccct ataaccgcaa ctgcggtctg gagcactttc cagaaggatt ttttcaaatc
    27661 ccactacgaa ggccgaagtc tccacagtat atttgaaaaa ggactctaag ggaaagtggt
    27721 tgcgggggcc ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct
    27781 gctccacccc gcgcctgaaa cgtggcaaat tctactcgtt ttgggtaaaa gatgcaaata
    27841 ctgctgggat ttggtgtacc gagacgggac gtaaaatctg caggcattat agtgatccat
    27901 gccacatttt gtcaacgttt attgctaata acgtgaatga atatccagtt cactttcatt
    27961 tgttgaatac ttttgccttc tcctgctctc ccttaagcgc attattttac aaaaaacaca
    28021 ctaaactctt cctgtctccg ataaaagatg attaaatgaa aactcattta ttttgcataa
    28081 aaattcagtg agagcggaaa tccaggctca tcatcagtta attaagcagg gtgttatttt
    28141 atgacgacga ttctcaagca tctcccggta ggtcaacgta ttggtatcgc tttttccggc
    28201 ggtctggaca ccagtgccgc actgctgtgg atgcgacaaa agggagcggt tccttatgca
    28261 tatactgcaa acctgggcca gccagacgaa gaggattatg atgcgatccc tcgtcgtgcc
    28321 atggaatacg gcgcggagaa cgcacgtctg atcgactgcc gcaaacaact ggtggccgaa
    28381 ggtattgccg ctattcagtg tggcgcattt cataacacca ccggcggcct gacctatttc
    28441 aacacgacgc cgctgggccg cgccgtgact ggtaccatgc tggttgctgc gatgaaagaa
    28501 gatggcgtga atatctgggg tgacggtagc acctataaag gaaacgatat cgaacgtttc
    28561 tatcgttatg gtctgctgac caatgctgaa ctgcagattt acaaaccgtg gcttgatact
    28621 gactttattg atgaactggg cggccgtcat gagatgtctg aatttatgat tgcctgcggt
    28681 ttcgactaca aaatgtctgt cgaaaaagcc tactccacag actccaacat gcttggtgca
    28741 acgcatgaag cgaaggatct ggaatacctc aactccagcg tcaaaatcgt caacccgatt
    28801 atgggcgtga aattctggga tgagagcgtg aagatcccgg cagaagaagt cacagtacgc
    28861 tttgagcaag gtcatccggt ggcgctgaac ggtaaaacct ttagcgacga cgtagaaatg
    28921 atgctggaag ctaaccgcat cggcggtcgt cacggcctgg gcatgagcga ccagattgag
    28981 aaccgtatca tcgaagcgaa aagccgtggt atttacgaag ctccggggat ggcactgctg
    29041 cacattgcgt atgaacgtct gttgaccggt attcacaacg aagacaccat tgagcagtat
    29101 cacgcgcatg gtcgtcagtt gggccgtctg ctgtaccagg ggcgttggtt tgactcccag
    29161 gcgctgatgc tgcgtgactc tctgcaacgc tgggttgcca gccagatcac tggtgaagtt
    29221 accctggagc tgcgccgtgg gaacgattat tcaatcctga ataccgtctc agagaacctg
    29281 acctacaagc cagagcgtct gacgatggaa aaaggcgact cggtgttctc gccagatgat
    29341 cgtattggtc aattgaccat gcgtaatctg gatatcactg atacccgaga gaaacttttt
    29401 ggttatgcca aaactggcct gctctcctcc tctgccactt caggcgtgcc gcaagtggag
    29461 aatatggaaa acaaaggcca gtaattcgac atcaaccctg cccttcaggg gtggggttac
    29521 cttgcttatc ctctgtaaac tcctttctga gcatcacata atcgaaaaca tcatggtttg
    29581 catccacaac ccgtgagtct cctttatagt gtgcaatcac ttcctcaagc gtttgcggct
    29641 gttccggctt tgttaccccc atccacgcat taataaggta attattgtaa gctgtcgaaa
    29701 agatgttgtt ttccgttgtg cgatccacgc catccccaag aacggggcta taccagataa
    29761 acatcgggac atgatatgcc tgctggctag cctccctgcc tccatgaaaa tagacgttct
    29821 tcttcgtagg gtcacgttcc aggccgtggt cggcaaaata catgaccgag gcgcggcgat
    29881 cttttaataa ttcaaaaacc tgacctagca aactatctgt gtaatgaatg gagttgtcat
    29941 agcaggcatc ctgatcgtcc tgcggttgaa acacggcgct ggattgcgga taggcgctac
    30001 aagccggttc atggcttcca tttaaatgaa gaacaatcag tttcttttgc tgcgtatttt
    30061 gctgtaatgc ttgcgataaa tgcggc
//
LOCUS       DAZGTR010000050        29850 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGTR010000050 DAZGTR010000000
VERSION     DAZGTR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.050"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(385..2454)
                     /gene="glyS"
                     /locus_tag="ACHLCY_002426"
     CDS             complement(385..2454)
                     /gene="glyS"
                     /locus_tag="ACHLCY_002426"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP0369672.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2464..3375)
                     /gene="glyQ"
                     /locus_tag="ACHLCY_002427"
     CDS             complement(2464..3375)
                     /gene="glyQ"
                     /locus_tag="ACHLCY_002427"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP0369673.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3470..3769)
                     /gene="ysaB"
                     /locus_tag="ACHLCY_002428"
     CDS             complement(3470..3769)
                     /gene="ysaB"
                     /locus_tag="ACHLCY_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP0369674.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLSMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3944..4939
                     /gene="wecH"
                     /locus_tag="ACHLCY_002429"
     CDS             3944..4939
                     /gene="wecH"
                     /locus_tag="ACHLCY_002429"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP0369675.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4981..5418)
                     /gene="yiaA"
                     /locus_tag="ACHLCY_002430"
     CDS             complement(4981..5418)
                     /gene="yiaA"
                     /locus_tag="ACHLCY_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP0369676.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5464..5805)
                     /gene="yiaB"
                     /locus_tag="ACHLCY_002431"
     CDS             complement(5464..5805)
                     /gene="yiaB"
                     /locus_tag="ACHLCY_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP0369677.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5974..7428)
                     /gene="xylB"
                     /locus_tag="ACHLCY_002432"
     CDS             complement(5974..7428)
                     /gene="xylB"
                     /locus_tag="ACHLCY_002432"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP0369678.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7500..8822)
                     /gene="xylA"
                     /locus_tag="ACHLCY_002433"
     CDS             complement(7500..8822)
                     /gene="xylA"
                     /locus_tag="ACHLCY_002433"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP0369679.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELMLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMAVALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9188..10180
                     /gene="xylF"
                     /locus_tag="ACHLCY_002434"
     CDS             9188..10180
                     /gene="xylF"
                     /locus_tag="ACHLCY_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0369680.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            10258..11799
                     /gene="xylG"
                     /locus_tag="ACHLCY_002435"
     CDS             10258..11799
                     /gene="xylG"
                     /locus_tag="ACHLCY_002435"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP0369681.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11777..12958
                     /gene="xylH"
                     /locus_tag="ACHLCY_002436"
     CDS             11777..12958
                     /gene="xylH"
                     /locus_tag="ACHLCY_002436"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP0369682.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            13036..14214
                     /gene="xylR"
                     /locus_tag="ACHLCY_002437"
     CDS             13036..14214
                     /gene="xylR"
                     /locus_tag="ACHLCY_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP0369683.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14322..15146)
                     /gene="bax"
                     /locus_tag="ACHLCY_002438"
     CDS             complement(14322..15146)
                     /gene="bax"
                     /locus_tag="ACHLCY_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP0369684.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSNSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15466..17496
                     /gene="malS"
                     /locus_tag="ACHLCY_002439"
     CDS             15466..17496
                     /gene="malS"
                     /locus_tag="ACHLCY_002439"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0369685.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTQPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASQVRVCPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDASAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKAIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCIAQMDMTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17674..18927
                     /gene="avtA"
                     /locus_tag="ACHLCY_002440"
     CDS             17674..18927
                     /gene="avtA"
                     /locus_tag="ACHLCY_002440"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP0369686.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(19079..19552)
                     /gene="ysaA"
                     /locus_tag="ACHLCY_002441"
     CDS             complement(19079..19552)
                     /gene="ysaA"
                     /locus_tag="ACHLCY_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0369687.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19654..20502)
                     /gene="yiaJ"
                     /locus_tag="ACHLCY_002442"
     CDS             complement(19654..20502)
                     /gene="yiaJ"
                     /locus_tag="ACHLCY_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP0369688.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20703..21701
                     /gene="yiaK"
                     /locus_tag="ACHLCY_002443"
     CDS             20703..21701
                     /gene="yiaK"
                     /locus_tag="ACHLCY_002443"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP0369689.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21713..22180
                     /gene="yiaL"
                     /locus_tag="ACHLCY_002444"
     CDS             21713..22180
                     /gene="yiaL"
                     /locus_tag="ACHLCY_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP0369690.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            22298..22771
                     /gene="yiaM"
                     /locus_tag="ACHLCY_002445"
     CDS             22298..22771
                     /gene="yiaM"
                     /locus_tag="ACHLCY_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP0369691.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22774..24051
                     /gene="yiaN"
                     /locus_tag="ACHLCY_002446"
     CDS             22774..24051
                     /gene="yiaN"
                     /locus_tag="ACHLCY_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP0369692.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            24064..25050
                     /gene="yiaO"
                     /locus_tag="ACHLCY_002447"
     CDS             24064..25050
                     /gene="yiaO"
                     /locus_tag="ACHLCY_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP0369693.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            25054..26550
                     /gene="lyxK"
                     /locus_tag="ACHLCY_002448"
     CDS             25054..26550
                     /gene="lyxK"
                     /locus_tag="ACHLCY_002448"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP0369694.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHTLPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26547..27209
                     /gene="ulaD"
                     /locus_tag="ACHLCY_002449"
     CDS             26547..27209
                     /gene="ulaD"
                     /locus_tag="ACHLCY_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0369695.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKVLS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            27202..28062
                     /gene="sgbU"
                     /locus_tag="ACHLCY_002450"
     CDS             27202..28062
                     /gene="sgbU"
                     /locus_tag="ACHLCY_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP0369696.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            28056..28746
                     /gene="araD"
                     /locus_tag="ACHLCY_002451"
                     /pseudo
     CDS             28056..28746
                     /gene="araD"
                     /locus_tag="ACHLCY_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
     gene            complement(28778..28879)
                     /locus_tag="ACHLCY_002452"
                     /pseudo
     CDS             complement(28778..28879)
                     /locus_tag="ACHLCY_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28883..29146
                     /locus_tag="ACHLCY_002453"
                     /pseudo
     CDS             28883..29146
                     /locus_tag="ACHLCY_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29093..29833)
                     /gene="yiaT"
                     /locus_tag="ACHLCY_002454"
     CDS             complement(29093..29833)
                     /gene="yiaT"
                     /locus_tag="ACHLCY_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP0369697.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITNSPMIDRNESFTFITGVSWRF"
BASE COUNT         7363 a         7646 c         7477 g         7364 t
ORIGIN      
        1 gaaaaataaa agcgtgaaac aaatcactat taaagaaagt aatctatatt tctgcggcat
       61 tcccagcccc ctgtgttgat ttccaacgag taagaggcta accttatggt gttcaggcat
      121 agggtagcct cactttgatt tatagtcagg tggggctttt ctctgtctgc ctttcggtga
      181 atacctgaga caaacagtct caagcacccg tggctattct agcttaataa gtttgtttct
      241 tctccttgat ataatcctaa aaaaactcat aaaattaata tatgagataa tctttattca
      301 gcagaagatt attaaaggtt gctgtattat ttagcgataa taaaaaaagc ctgccagatg
      361 gcaggctttt taataacggc gttattactg caacagcgaa atatccgcaa cgcgcaggaa
      421 cagttcgcgc agtttctcca gcatggtcag acggttgaga cgcaattctt tgtcatcaac
      481 catgaccatc actttatcga agaaagcatc aaccggctca cgcagctctg ccagttcgac
      541 cagcgcatcc tggtaacgac cttccgcaaa gtacggctcc agcttgtcac gtagcaccac
      601 aacctgcatc gccagtttaa tttcttccgg ctctttcagg gtagaggcat tcacgcggtc
      661 actcagcact tcgtcagatt tcgccaaaat gttggagaca cgcttgttcg ccgccgccag
      721 tgcagcagct gcttcaaggg tacggaagtg cgataccgct ttcatgcggg catcgaaatc
      781 agccggacga gtcggacgac gcgccagtac cgcctggatg gtgtcaacgg tgtaaccttc
      841 gtcctgatac caggcgcgga agcgaccgag cataaagtcg ataacatcat caaccacgtt
      901 ggcgttagtc agcttatcgc catacagacg cactgcttct tcggtcagcg tttgcagatc
      961 aaggttgagg ttcttctcaa cgataattcg cagcacgcca agtgcggcac gacgcagcgc
     1021 aaacgggtct ttgtcgcctt tcggatgctg gccgataccg aagatacccg ccagagtatc
     1081 catcttgtca gcaatcgcca gcgcacaggc taccgggtta gacggcaggt catcaccggc
     1141 aaagcgcggc tgatactgct cattcagcgc caccgcgaca tcttccgctt cgccatcgtg
     1201 acgcgcatag tgcatcccca taacgccctg ggtgtcggtg aactcgaaga ccatgttggt
     1261 catcaggtca cacttagaca gcaggcccgc acgggttgcg tggttaacgt cagcgccaat
     1321 ctgttcagca atccagccag ccagcgcctg gatgcggtca gttttgtcgc gcagcgtacc
     1381 cagctgttgc tggaacagca cggtttgcag gcgcggcagg ttatcttcca gacgtttttt
     1441 acggtcggta ttgaagaaga actcggcatc cgccagacgt ggacgaacga ctttctcgtt
     1501 accagagata atttgctgcg gatctttcga ttcgatgttg gcaacgaaga tgaagttcgg
     1561 cagcagtttg ccatcgttcg catacaccgg gaagtatttc tggtcacctt tcatggtgta
     1621 aaccagggct tcagccggca ccgcgaggaa tttctcttcg aattttgcgg tcagaacgac
     1681 cggccactcc accagcgaag ccacttcttc cagcaggctt tcgcttaagt cagcgttacc
     1741 gccaatctta cgcgctgctt cttcggcatc ggctttaatc ttcgctttac gttcttcgta
     1801 atcggcgatg actttcccgc gctcacgcag aatttccgga tactgatcgg cgttgtcgat
     1861 ggtgaattcc ggttcgccca taaagcggtg gccgcgaatc acgcgatcgg actgaatgcc
     1921 cagaatggtt gccggaatga ctttgtcgcc cagcagcagg gtcacggtgt gcaccggacg
     1981 cacgaagtgc acgtcgcttg cgccccaacg catcagtttc gggatcggca gtttcgccag
     2041 agaagtcgca accatattcg gcagcagtgc ttcggtgctt tcgcccttca catgggcgcg
     2101 atacagcagc cattcgcctt tatcggtagt cagacgctca gcctggtcaa cggtaatacc
     2161 gcaaccacgc gcccaacctt ctgccgcttt gctcggtttg ccttcagcgt cgaacgcctg
     2221 ggcaatcgcc gggccgcgtt tttcgatttc acgatccggt tgcgcttccg ccaggttagc
     2281 tactttcagc gccagacgac gcggagcagc aaaccattga acggtgccgt gtgcgaggcc
     2341 agcgttatcc agctccgcag taaaattcgc agcaaaggac tcagccaggc tgcgcagtgc
     2401 ttttggtggc agttcttcag tgccgatttc caccagaaaa gttttctcag acatagccgc
     2461 ctcttactta tctttgttgc acatcgggaa gccgagggct tcacgggaag cgtagtatgc
     2521 ttctgccact gctttggtca gggtgcgaat gcgcagaatg tagcgctgac gctcggtgac
     2581 ggagatggct ttacgcgcat ccagcaggtt gaagctgtgg gcggctttca gaatacgctc
     2641 gtaggctggc agcggcagcg gattttccag cgccagcagc tgctgcgctt ctttctcgta
     2701 ctgctcaaag caggtgaaca ggaagtccac atccgcgtat tcgaagttgt aagtggactg
     2761 ctccacttcg ttctgatgga acacgtcgcc gtaggtggtt ttacccagcg ggccgtcgct
     2821 ccagaccagg tcgtaaacgc tgtctacgcc ctgaatgtac atggccagac gttccagacc
     2881 gtaggtgatc tcgccggtaa ccggtttaca ctccagacca ccaacctgct ggaagtaagt
     2941 gaactgcgtc acttccatgc cgtttagcca cacttcccag cccagtcccc aggcacccag
     3001 agtcgggttt tcccagttat cttccacgaa acggatgtcg tgaatagtcg ggtccatgcc
     3061 cagctctttc agagaaccga ggtacagctc ctgaatattg tccggcgatg gcttaatgac
     3121 cacctggaac tgatagtagt gctgtaaacg gttggggttt tcgccgtagc gaccatcggt
     3181 cgggcgacga gaaggctgaa cataagcagc cgccatcggt tctggcccca gcgcgcgcag
     3241 acaggtcatt gggtgagagg ttcccgcgcc gacttccatg tccaatggtt gaacaatggt
     3301 gcagccctgg cgagcccagt aatcctgtaa ggtcaggatc aagccctgga aggtcctggt
     3361 atcaaacttt tgcatattat ttcgtgctgg atacgtgtgg atttaaagga agggatcagt
     3421 atacccgctg gatggaagat atacagtacg aaacgggaaa aagcagggct taacgcatgg
     3481 aaagatgcaa aaaatggcca tctgcgtcaa aagaacagac aaactgctct ttacggaagg
     3541 tataaccgcg catttcatag cttccctgga actgttcgaa ggcggtgacg tcgatttttt
     3601 gctcgccggt gttataacgt tttgccgcct gatccttgca taacgcttcc atactcaacg
     3661 aacgcagagg atcaaccttt acccgttgtg ctttctgcac gggtgacggt gtagaacaac
     3721 ccactagcac cataagcgcc attgccggaa agaaagcgtt catcatcatt aaattaccgt
     3781 ctggtttgca ggtgagtctt attatttata tcggtatagt ggctgatatc ttacgaattt
     3841 ccttaagcct ctataagctg tagtgataaa actcagatta ttccagtgcg ccaaccaaat
     3901 gatttatcgg tcagactttt tttaaccaga tacagagaca cagatgcagc ccaaaattta
     3961 ctggattgat aacctgcgag ggatagcgtg tttaatggtg gtgatgattc acaccactac
     4021 ctggtatgtg accaatgctc atagtgttag ccccgtcaca tgggatatcg ccaatgttct
     4081 gaattctgcc tctcgtgtca gcgtgccgct atttttcatg atttccggct atctcttttt
     4141 tggcgaacgc agcgcccagc cgcgccattt cttgcgtatc ggcttatgtc tgatttttta
     4201 tagcgcaatc gcactgctct acattgcgct gtttacctcc atcaatatgg agttagcgct
     4261 gaaaaacctg ctgcaaaagc cagtgtttta ccacttgtgg tttttcttcg cgattgcggt
     4321 gatttatctg gtttcaccgc tgattcaggt gaagaacgtc ggcggaaaaa tgttgctggt
     4381 gctaatggtg gtgattggca ttatcgctaa cccaaacaca gtgccgcaga aaattgacgg
     4441 ttttgaatgg ctgccaatta acttatatat caatggcgat actttttact acattctgta
     4501 tggcatgttg ggccgcgcta tagggatgat ggacacacag cataaagcac tgtcgtgggt
     4561 gagcgccgcg ctgtttgcga cgggggtttt tattatctct cgcgggacat tatatgaatt
     4621 gcagtggcgc ggaaattttg ccgatacctg gtatctttac tgtgggccga tggtttttat
     4681 ctgcgcaatc gcgctattga ctctggttaa aaacacgctg gatacgcgta ccattcgcgg
     4741 acttggctta atctcccgcc attcattggg tatatacgga ttccacgcct tgattatcca
     4801 tgcgctgcgc acccggggaa ttgagcttaa aaattggcca atactggata ttatttggat
     4861 cttttgcgcg acgttggcag cgagtttgtt actttctatg ctggtacaac gaatcgacag
     4921 aaacagatta gtgagttaag taaaagcccg ttcacattgg actgaccggg cttacgtgaa
     4981 ttattcgctg tattcttcct gggtaacctg tgtttctttt ggcgggttta ttccggtatc
     5041 acgaatattc ttctgcaccg ctactgcacc aaacaagctt aagaagaaag ccagtccata
     5101 aaaacctttt tcactgagta ataatgtcgc attccacaga cctaccatca gtaatgcaac
     5161 agagataata aagacagtca ggcaggtcat ataataaatg gaagtggtcg gtatgccttc
     5221 atacttgtcc cgaacggtct tttgataaga cgcagcagaa aacagtccta acaccagtac
     5281 ggcaaaataa tacccttttt catttaactg catctctgca ttccatagcc ctaacagata
     5341 ggtaacgata ccaccaacga gagctatcca tgacacaata ctaaaggccg gtgaataggt
     5401 tgatattttg ttgtccatca cagtattcct ttcattcctg aatgtgtaag agctttatgt
     5461 tgctcatacc gagcgaaaca aacgcatttg accaaacaag cagacaaaaa aggcgatggg
     5521 gtagatcacc atttcataag cattgatagg tgcatttaaa acaccgataa acaagagacc
     5581 gatagtgatt aacgcaacta actggcagat atgggtaaaa aaattatcca gttgcccgag
     5641 tttttctgcg cgaagatatg catagttacc aaaagttgct gtcattaaca cgccaagaaa
     5701 atagcctttt ccacttagca atgggcatga gatccatagc ccaaccagat aaaccagcgc
     5761 cccgacaaca aataatagtt ttgccacagt ttttgaggtt ttcataacga tctccatatc
     5821 taccagcgat acattacgag taaccaacga aagacaaaac tgaaaaatgc cattaacaaa
     5881 tgattttcag aataaattca tactaaatat taattaatta gtgagatata tagatgtgaa
     5941 ttatccccca cccggtcagg caggggataa cgtttacgcc attaatggca gaagttgctg
     6001 atagaggcga cggaacgttt cacgtcgtgg ctgataagcg gcataacgct gcgcatctgg
     6061 tagatgcgac tgttctaacg gtagctgcgg caacaactca atgagcgatt tctctggatt
     6121 cgccgcaatc tgcgccagcc ttgctgcgcc cagtgctggc cccacatccc cccccgtacg
     6181 gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact cactacgcgc
     6241 cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca cgacatccat
     6301 gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt cattggggcc
     6361 atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt gtggtgtacg
     6421 ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag cctgttgagc
     6481 tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcgaccc aatccagaca
     6541 cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg catggcaaaa
     6601 gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa aatagacccc
     6661 cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac caactgcacc
     6721 agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc acgctttcgc
     6781 aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg gcatctggtc
     6841 acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg cgacatccag
     6901 ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca tacgcagacg
     6961 caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg gctcatgccg
     7021 ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc cggtaatcac
     7081 tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc gcccgtcgtt
     7141 ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg ctccatgcat
     7201 ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc ccagagcttt
     7261 cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc agagtggatg
     7321 cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct gctcgttgag
     7381 caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat cgatcgttcc
     7441 ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac agttagccgt
     7501 tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg accactctga
     7561 tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag tgacatttgg
     7621 cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg cgcgatgcgt
     7681 ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccac tgccatcgta
     7741 tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg acgacgtact
     7801 ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat ttcatacatc
     7861 accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc cagttgcgca
     7921 tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt ggctatttca
     7981 tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag tttaatctct
     8041 ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta atcatattga
     8101 tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctggaa gccgatttta
     8161 tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc ctgacgcaag
     8221 tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata gttttcaccg
     8281 cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca gctgaagact
     8341 tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa gcagttagcg
     8401 gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac atcaaccatt
     8461 tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac atccacatcg
     8521 tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac atctgcttta
     8581 cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa cgcccccaca
     8641 ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc aaaacgcaag
     8701 tgctcttcca tacgcttacc caacatcagt tcgtcgggat tgtagtgacg gaatgctaac
     8761 gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa ataggcttgc
     8821 atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta atattgggca
     8881 ctccctttca gttgctcaat tatgttattt cacactgcta ttgagataat tcacaagtgt
     8941 gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga agataaaaat
     9001 gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag aaataaacca
     9061 aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt tgctaaaaat
     9121 tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact acagaaggcc
     9181 ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc tgcttaccaa
     9241 cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc tccgtcttga
     9301 acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg gcgcgaaagt
     9361 atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg aaaacatgat
     9421 aaaccggggt gttgatgttc ttgtcattat tccgtataac ggtcaggtat taagtaacgt
     9481 tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgatcgta tgattaacga
     9541 tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac tgcaggcaaa
     9601 agccctggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct cgccggtaga
     9661 taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt acgttgattc
     9721 cggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg aaaacgcatt
     9781 gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg tagttgcctc
     9841 aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt tatcagggaa
     9901 agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg ctgccggtac
     9961 gcaaactatg acggtgtata aacctattac gttgttggca aatactgccg cagaaattgc
    10021 cgttgagttg ggcaatggtc aggaaccaaa agcagatacc acactgaata atggcctgaa
    10081 agatgtgccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca tcaaagacac
    10141 ggtagttaaa gacggattcc acaaagagag cgagctgtaa gcgttacgcc ccggcgcgga
    10201 gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg agtcgttatg
    10261 ccttatctac ttgaaatgaa gaatattacc aaaaccttcg gcagtgtgaa ggcgattgat
    10321 aacgtctgct tgcggttgaa tgctggcgaa atcgtctcac tttgtgggga aaatgggtct
    10381 ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc ctacgaaggc
    10441 gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac cgaacgcaaa
    10501 ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt gctggaaaat
    10561 atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct gatgacgcta
    10621 cgctgtcaga agctgctcgc acaggtcagt ttatccattt cacctgatac ccgcgttggc
    10681 gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa taaacaggtg
    10741 cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac gtcggtttta
    10801 ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat ttcgcacaaa
    10861 ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg acagcacatt
    10921 ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat ggtcgggcga
    10981 gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat attacgtatt
    11041 gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa tgatgtctcg
    11101 ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc cggacgtacc
    11161 gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa aatttatatt
    11221 gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg gattgcgatg
    11281 gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg taaaaatatt
    11341 accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga cgcggaagag
    11401 caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc ccccgatctt
    11461 gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgctcg ctgtctgtta
    11521 cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtatcgatat tggcgcgaaa
    11581 tacgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt tattgtcatc
    11641 tcttccgaat tacctgaagt gctcggcctt agcgatcggg tactggtgat gcatgaaggg
    11701 aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat ggaagccgca
    11761 ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg ccgtaccgac
    11821 atccggtggc ttttccgggc tgaaatcact gaatttgcag gtcttcgtga tgattgcagc
    11881 tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact taagcgcccg
    11941 taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg taggaatggt
    12001 gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg ggctattagg
    12061 tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta ccatcattgt
    12121 gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg cgtaccgcaa
    12181 agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca tactcattgg
    12241 catcaccaac ggcacgactg tttcccccac cagcgccgcg atgtcacaaa ttgggcaaag
    12301 ctatctacct gccagcactg gcttcatcat tggcgcgctt ggcttaatgg cttttgttgg
    12361 ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttgcagtctc cggcctctac
    12421 cgcagtagtc ggtcgccagg ctttaaccgc tatcatcgta ttaggcgcaa tctggctgtt
    12481 gaatgattac cgtggcgttc ccactcctgt tctgctgctg acattgctgt tactcggcgg
    12541 aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg gcggcaatct
    12601 ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg tgttcgcgat
    12661 taacggatta atggtagcca tcgccggatt aatccttagt tctcgacttg gcgctggttc
    12721 accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga ttggcggcac
    12781 cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat ttatcatggc
    12841 ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt atatcgttaa
    12901 aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc gttcttgatt
    12961 ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt tgcatgattc
    13021 agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt caatgccaat
    13081 aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc atcacaatca
    13141 gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat caaggactgg
    13201 ttaggagatg gcgtcattgc cgacttcgac gacaaacaga tcgagcaagc gctggctgat
    13261 gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag ttacccaccc
    13321 gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt gcatttaaaa
    13381 gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg caaacgttgg
    13441 gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta tcgcggagtg
    13501 gtttatcagg ggttagaaac cgcgccagag aactggcaac atgcgcaaaa tcggctggca
    13561 gactggctac aaacgctgcc accgcaaacc gggattattg ccgttactga cgcccgggcg
    13621 cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa attatgcgtg
    13681 attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct ttcttcggtc
    13741 gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg attattagat
    13801 aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat tgaacggcgc
    13861 tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca ttacattcgt
    13921 aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg gatctcgcgc
    13981 tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc aatgattcat
    14041 gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc gatcaatgag
    14101 atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt taaaaaagca
    14161 tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt gtagcgatga
    14221 tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg agcaaaagcc
    14281 ttatccgacc gacaaatcag ataaggctca aggaaatgca attacatatg cgccgcgatt
    14341 aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt ccctttggtc
    14401 gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc cgctttacgc
    14461 agctgcgcac gcgatttacg gaatgaagag taagccggat gcgtattgag gttagtgaca
    14521 taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac tttacccggc
    14581 gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttgtt gttgcgcgcc
    14641 agctttgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat actggtgggg
    14701 ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt accagaccac
    14761 ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg tgaccattgg
    14821 ccctgatact gttttgaaat cagccagtta cgttccgcag taatggctgc attttggctg
    14881 gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc ggaagggtat
    14941 tttcgcaaat caggaagtga attactcttt gcactattgc gagaatactc ttgtttactg
    15001 cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt tgttgtgtgc
    15061 gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat catcgcccca
    15121 tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc attccaacac
    15181 cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat agctgtcaag
    15241 agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat tcagattggc
    15301 gctaaatcgc agaaaatgtg gggtttatcg caaaattcag ccgttttttg cgcgagatcg
    15361 ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg tttggcttat
    15421 cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccgccatgaa actcgccgcc
    15481 tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc tccggggttc
    15541 cctgccttta gtgaacaggg aacgggaaca tttgtcagcc acgcgcagtt gcccaaaggt
    15601 acgcgtccac taacgctaaa ttttgaccaa cagtgctggc agcctgcgga tgcgataaaa
    15661 ctcaatcaga tgctttccct gcaaccttgt agcaacacgc agcctcaatg gcgattgttc
    15721 agggacggcg aatatacgct gcaaatagac acccgctccg gcacgccaac attgatgatt
    15781 tccatccaga acgccgccga accggtagca agccaggtcc gtgtatgccc gaaatgggat
    15841 ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc cgtacgggat
    15901 tattacagcc agcaaattgc gatagtgaag aacggtcaaa taacgttaca acccgctgcc
    15961 accagcaacg gtttactcct gctggaacgg gcagaaactg acgcctctgc ccctttcgac
    16021 tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg cgatcccagt
    16081 aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac ttttcacggc
    16141 ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg cgttaatgct
    16201 ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg tacaaaaggc
    16261 gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa tcttgatgcc
    16321 aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca gcgcggtatt
    16381 cgtattctct ttgatgtcgt gatgaaccac accggctatg ccacgctggc ggatatgcag
    16441 gagtatcagt ttggcgcgtt atatctttct ggtgacgagg tgaaaaaaac gctgggtgaa
    16501 cgctggagcg actggaaacc tgcagccggg caaacctggc atagctttaa cgattacatt
    16561 aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat ccgtaccgat
    16621 atcggcgatt acgacaatcc tggattcgac gatctcacca tgtcgctagc ctttttgccg
    16681 gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa aaacaaaacg
    16741 gatacccacg ctaaagccat cgacggcttt acccctcgcg attacttaac ccactggtta
    16801 agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc caaacatgtt
    16861 gagttgcccg cctggcagca actgaaaacc gaagccagcg ccgcgcttcg cgaatggaaa
    16921 aaagctaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg tgaagcctgg
    16981 ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat gatcaatttc
    17041 gattatcagg agcaggcggc gaaagctgtc gattgtattg cgcagatgga tatgacctgg
    17101 cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc gtcgcatgat
    17161 acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt agcgccaggc
    17221 gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac aggttctgat
    17281 ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa atctgccgcc
    17341 aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc aattggcgcg
    17401 ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga gcatggcgac
    17461 gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc cgttcgtcag
    17521 tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt gcaggataaa
    17581 gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt tattgttacc
    17641 ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt tggtgacaaa
    17701 tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg tttacgcacc
    17761 cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat gcaggactac
    17821 ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc actgtgcaat
    17881 tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat gctgcgcgag
    17941 aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag ccagagcgcg
    18001 tttttctact tatttaatct gtttgccgga cgccgtgccg atggtcgggt caaaaaagtg
    18061 ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga agaagatctg
    18121 tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa ataccacgtc
    18181 gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc ccggccgacg
    18241 aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc gctggcgaat
    18301 caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc gggtatcatc
    18361 ttcagtgaag cgcgcccgct atggaatccg aatatcgtgc tgtgcatgag tctttccaag
    18421 ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat catcaccgcc
    18481 atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc ggcgatgatg
    18541 tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat caaaccgttt
    18601 tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc ggaaaatcgc
    18661 tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa ggatttgccc
    18721 attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat ggtgccgggg
    18781 cacaacttct tcccagggct ggataaaccg tggccgcata cgcatcaatg tatgcgcatg
    18841 aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc ggaagagata
    18901 gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt atcaggccta
    18961 catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag gcgtttacgc
    19021 cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga aaaacgtttc
    19081 aaacaggctg ctgccgtagc cgatgccgct gcactttctc gacatccatg cactgcaacg
    19141 cctttgtcgg gcaggcttca acacaggccg gtcccgtctc ccgatgccag cacagatcgc
    19201 acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca aacgggcaag
    19261 ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata tgcccatgtt
    19321 cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca cactgatgac
    19381 aggctactgc cgtggtccag ctgtggtctt taatgacacg aatacgggaa ataaactcgt
    19441 ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa gccacttcac
    19501 aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc atgatcactc
    19561 ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga tccaacgaag
    19621 gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca ggtcatccct
    19681 gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg gtttcaggag
    19741 atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca cggcgtacgg
    19801 cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga gttcgttttc
    19861 ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga acatcgcggg
    19921 cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt tttcccagta
    19981 tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg cggaacagta
    20041 gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg tgggttccag
    20101 cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg tttcaccagt
    20161 ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca gcgaagacag
    20221 cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg cggcgggcgc
    20281 ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg tactcttatt
    20341 taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt tgctcaaaat
    20401 ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct ctttttcctg
    20461 cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc gtcctgatgg
    20521 tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca cttgattttt
    20581 atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat atatttgaaa
    20641 tcaagtttcg catattgaga ttttaagcca aaaaagcgat caaaaaaaca aggaagcctg
    20701 ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta atttcacgcg
    20761 gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc accgaatccg
    20821 gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg gaaaacggcg
    20881 atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt gaacagtggg
    20941 atgcccagcg ttcgatcggc aacctgacgg cgaaaaagat gatggatcgc gccattgaac
    21001 tggctgccga tcacggtatt ggtctggtgg cacttcgtaa tgccaaccac tggatgcgcg
    21061 gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc tggaccaact
    21121 ccatcgccgt aatgccgccg tggggcgcaa aagagtgtcg cattggcact aacccgctga
    21181 tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg atgttctctt
    21241 acggcatgtt agaagttaac cgcctggcgg gccgtcagct cccggtcgat ggtggctttg
    21301 atgatgaggg caatttgacc aaagaacctg gcgttatcga gaagaatcgc cgcattttgc
    21361 cgatgggcta ctggaaaggt tctggcatgt cgattgtgct ggatatgatc gctactctcc
    21421 tttccgacgg cgcatcggtt gccgaagtca cccaggacaa cagcgacgaa tacggcattt
    21481 cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc cgcgatgcca
    21541 agctgcaacg tatcatggat tacgttacta ccgctgaacg cgctgacgaa aaccaggcca
    21601 tccgcttacc cggccacgaa tttactaccc tgctggccga aaaccgccgt aacggtatta
    21661 ccgttgatga cagcgtgtgg gccaaaatcc aggcgttata aggagataag tcatgatttt
    21721 tggacatatc gcgcagccca atccgtgccg tttgcccgcc gccattgaaa aggcgctcga
    21781 ttttctgcgc gctaccgatt tcaacgccct ggagccgggc gttgtcgaaa tcgacggcaa
    21841 aaatatttat gcgcaaatta tcgatttaac cacacgcgaa gcggtggaaa accgtccgga
    21901 agtgcatcgt cggtatatcg atattcagtt tctggcgtgg ggtgaagaga aaatcggcat
    21961 tgctattgat acgggaaata ataaagtcag cgaatcacta ttagagcagc gcgatattat
    22021 tttttatcat gacagtgaac atgaatcatt tatagaaatg ataccgggca gctacgcaat
    22081 tttctttccg caggatgttc accgaccagg ttgtattttg caaacagcct ctgagatacg
    22141 aaaaattgtg gttaaagtcg cattaacggc gctgaattaa tcgaataatc gtctacgcca
    22201 gaacgactgg gtaatgttat tgctctaact atattaataa tttaggttgc tgcacatgca
    22261 acttgaatta tgacagacat agcctcaagg aatagctatg aaaaaaatac ttgaagcaat
    22321 actggcgatt aatctcgccg tactttcctg tattgtgttt ataaacatta ttttaagata
    22381 tggttttcag acaagcattt tatctgtaga tgaattgtca cgttatttat ttgtctggtt
    22441 aacatttatt ggcgcgattg tggcttttat ggataacgcc cacgttcagg tcacctttct
    22501 ggtggagaaa ctctcccccg catggcaacg acgagtcgct ctggttaccc attccttaat
    22561 cttatttatt tgtggcgcac tggcctgggg cgcaacgcta aaaacaattc aggactggag
    22621 cgattattca ccgatcctcg gtttacccat cggcctgatg tatgccgctt gtctgcccac
    22681 cagcctcgtt atcgcattct ttgaactgcg tcatttatat caactcatca cgcgtagcaa
    22741 ttctctaacg tcgccaccgc aaggagctta atcatggctg tgctgatttt tctgggctgt
    22801 ctgttgggtg gtattgctat cggtttgcct attgcctggg cactgttgtt gtgcggggcg
    22861 gcgttaatgt tctggctgga catgtttgat gtccagatca tggcacaaac gctggtgaac
    22921 ggagccgata gcttctccct gctggcgatt ccgttctttg tgctggcggg tgaaatcatg
    22981 aatgcgggcg gcttgtcaaa acgcattgtt gacctgccga tgaaactggt ggggcataaa
    23041 ccaggcgggc tgggctacgt cggcgtgctg gcagctatga ttatggcgag cctttctggt
    23101 tctgccgttg cggataccgc cgccgttgcc gctctactgg tgccgatgat gcgcagcgcc
    23161 aactacccgg tcaaccgggc ggcggggctg attgcctctg gcggcattat cgcgccaatt
    23221 attccccctt ccattccgtt tattatcttc ggcgtttcca gcggattatc catcagtaag
    23281 ctgtttatgg cgggcattgc ccctggcatg atgatgggcg caacgctgat gcttacctgg
    23341 tggtggcagg ccagtcgcct taatttaccc cgccagcaaa aagcaacgat gcaggaaatc
    23401 tggcactctt ttgtctccgg tatctgggcg ctgtttcttc cagtcatcat tattggcggg
    23461 tttcgctccg gtctgtttac ccctaccgaa gccggggccg ttgctgcttt ctatgcgctg
    23521 tttgtcgcca cagttattta ccgtgaaatg accttcgcca cactttggca tgtactcatt
    23581 ggcgcggcga aaaccacctc agtggttatg tttctggtgg cctcggcaca agtttccgcc
    23641 tggctgatta ccattgctga actgccgatg atggtttctg atttactgca accgttggtc
    23701 gattcaccgc gtctgctgtt tatcgtcatt atggtggcta tcctgattgt cggcatggtc
    23761 atggatttaa cgccgacggt attaattctt accccggtat taatgccttt agttaaagag
    23821 gcaggaatcg atccgattta tttcggtgtc atgtttatca ttaactgctc aatcggctta
    23881 attacaccgc ctatcggtaa tgtacttaac gttatttcag gggtggcaaa actcaaattc
    23941 gatgatgcag tcagaggcgt tttcccttac gtcctggttt tatattcatt attagtcgta
    24001 tttgttttta ttcccgatct catcatcctc cctttgaaat ggattaatta aaaggaaaat
    24061 attatgaaat tacgctctgt aacctacgca ttattcattg ctggcctggc tgcattcagc
    24121 acatcttctc tggcggcgca atctttacgt ttcggttatg aaacctcaca aaccgactcg
    24181 caacatattg cggcgaaaaa attcaatgat ttattgcagg agagaaccaa aggcgagctg
    24241 aaattaaaat tgtttccgga tagcaccctc ggtaacgcgc aggcgatgat cagcggcgta
    24301 cgtggcggca ccatcgatat ggaaatgtcc ggctcgaata actttgccgg gttatcacca
    24361 gtgatgaact tgcttgatgt ccctttcctg ttccgcgata ccgctcacgc gcataaaacg
    24421 ctcgacggca aagtcggtga tgatctgaaa gcctcacttg aaggtaaagg actaaaagta
    24481 ctggcctact gggaaaacgg ctggcgcgat gtcaccaact cgcgcgcacc ggttaaaacc
    24541 cccgccgacc tgaaagggct gaaaattcgc accaacaata gcccgatgaa tatcgccgca
    24601 ttcaaagtct ttggcgctaa cccgatcccg atgccgtttg ccgaagtcta taccgggctg
    24661 gaaacccgca ctatcgacgc tcaggaacac ccgatcaacg tcgtctggtc agcaaaattt
    24721 ttcgaagtgc agaagtacct ttctctgacg caccacgcct attccccgct tctggtggtg
    24781 atcaacaaag cgaagtttga tggcttaagc ccggagttcc agcaagcgct aatttcatct
    24841 gcgcaggaag cgggtaacta ccagcgcaaa ctggttgccg aagatcagca aaaaatcatc
    24901 gacggcatga aagaagcggg cgtggaagtc atcaccgatc tcgaccgcaa agcctttagc
    24961 gacgcactgg gtactcaggt ccgcgacatg tttgtaaaag atgttccgca gggtgctgat
    25021 ctactgaaag ccgtggatga ggtgcaataa accatgacgc aatactggct ggggttagat
    25081 tgtggcggta gctggctgaa agccgggctg tatgaccgcg aaggccggga ggcaggcgtg
    25141 cagcgcctgc cgctgtgcgc attaagcccg cagccaggtt gggcagagcg cgatatggca
    25201 gaactgtggc aatgctgcat ggctgtcatt cgcgaactgc ttacccattc cggcgttagc
    25261 ggggaacaaa ttgtcggtat cggtatctcc gcacaaggaa aaggcttgtt tttgctggat
    25321 aaaaacaaca agccgctcgg taacgccatt ttatcctcgg atcgccgggc gatggaaatc
    25381 gttcgtcgct ggcaggaaga tggcatcccg gaaaaactct acccgctgac ccgacaaacc
    25441 ttgtggaccg ggcatccggt atcgctgtta cgttggctta aagagcacga accggaacgc
    25501 tacgcgcaaa ttggctgcgt gatgatgacg cacgactacc tgcgctggtg tttaaccggc
    25561 gtcaaaggct gtgaagagag caatatttcc gagtccaatc tctacaacat gagtcttggg
    25621 gaatatgacc cgtgcctcac cgactggctg gggattgctg aaatcaatca caccctgccg
    25681 cctgttgtcg gatctgccga aatctgcggg gagatcaccg ctcagacagc cgtactgacc
    25741 ggtctgaaag cgggtacgcc cgttgtcggc ggcctgtttg atgtggtttc caccgcactc
    25801 tgcgccggga tcgaagacga atttaccctt aatgcagtga tgggcacctg ggcggtgacc
    25861 agcggcataa cccgcggttt acgtgacggt gaagcgcatc cgtatgtcta tggtcgctac
    25921 gttaacgatg gtgaatttat cgttcacgaa gccagcccca cctcttccgg caacctcgaa
    25981 tggtttaccg cacagtgggg agaaatctcg tttgatgaga tcaaccaggc cgttgccagc
    26041 ttgccgaagg ccggtggcga tctctttttc ctgccgttcc tgtacggcag caacgccgga
    26101 ctcgagatga ccagcggttt ctacgggatg caggccattc acacccgcgc acacctgttg
    26161 caggccattt atgaaggggt ggtgttcagc catatgaccc atctcaaccg gatgcgcgaa
    26221 cgttttactg atgtgcacac cctgcgcgtc actggcggcc cggcgcattc cgatgtctgg
    26281 atgcaaatgc tggcggacgt cagcggtctg cgtattgagc tgccgcaggt ggaagaaacc
    26341 ggctgctttg gtgcagcact tgccgcccgc gtcggcaccg gggtttatcg cgatttcagc
    26401 gaagcccagc gtgatttaca gcacccggtg cgcaccctgc tgccggatat gactgtccat
    26461 cagctttacc aacaaaaata ccaacgctat cagcatctca ttgccgcact tcagggcttt
    26521 cacgcccgca tcaaggagca catattatga gccgaccact tctgcaactg gcactcgacc
    26581 actcatcact tgaagccgca cagcgcgacg tgacgcagtt aaaagacagc gtcgatattg
    26641 tcgaagcggg caccattctc tgtttaaacg aagggcttgg cgcggtgaaa gccttgcgcg
    26701 aacagtgccc tgacaaaatc atcgttgccg actggaaggt cgccgacgct ggtgaaacgc
    26761 tcgcgcaaca ggcatttggc gcaggcgcta actggatgac catcatctgc gccgcaccgc
    26821 tcgcgacggt ggaaaaaggc cacgcgatgg cgcaacgctg cggtggtgaa attcagatcg
    26881 agctgttcgg taactggacg ctggacgacg cccgcgactg gcatcgtatt ggcgtgcggc
    26941 aggcgattta tcatcgcggt cgtgatgcac aggccagcgg gcaacagtgg ggcgaagccg
    27001 atctggcacg catgaaggtg ctttccgata tcggccttga gctttccatt accggcggga
    27061 ttacccctgc tgacctgccg ctgtttaaag atattcgcgt gaaagcgttt attgccgggc
    27121 gagcactggc aggctcagca aatccggcgc aggtggctgg cgatttccat gcgcaaatcg
    27181 acgctatctg gggaggcaag cgtgcgtaat catccgttag ggatttatga aaaggcgctg
    27241 gcgaaagatc tctcctggcc ggagcggctg gtgctggcaa aaagctgcgg ttttgatttt
    27301 gtcgaaatgt cggtggatga aaccgacgaa cggctttcac gtcttgactg gagcaccgca
    27361 caaaggactt cgctggttgc cgcgatgatc gaaacgggtg ttgggatccc gtcgatgtgc
    27421 ctgtccgcac atcgtcgctt tccttttggt agccgtgacg acgcggtacg ccagcgagca
    27481 cgggagatca tgagcaaagc gattcgtctg gcacgcgatc tcggcatacg caccatccag
    27541 ctggcgggct acgacgtcta ttacgaagac cacgatgaag gcacccggca acgttttgct
    27601 gaagggctgg cgtgggcggt cgaacaggcg gcggcatcac aagtcatgct agcggtggag
    27661 attatggaca ccgcgtttat gaactccatc agtaaatgga aaaagtggga cgagatgctc
    27721 gcctcgccgt ggttcaccgt ctacccggac gtcggcaacc tcagcgcctg gggcaatgat
    27781 gttcccgccg aactgaaact gggcattgac cgtatcgccg cgatccacct gaaagacacc
    27841 cagccagtta ccgagcaaag tcccggacag ttccgcgacg tgccgtttgg cgaaggctgc
    27901 gtcgatttcg ttggcatctt caaaacgctg cataaactga actatcgcgg ttctttcctg
    27961 attgagatgt ggaccgaaaa agccaaagag ccggtgctgg agattattca ggcgcggcgt
    28021 tggattgaag cgcgtatgca ggaggctgga tttatatgtt agagcaactg aaagccgacg
    28081 tgctggcggc gaatctggag cttcccgctc accatctggt gacgttcacc tggggcaatg
    28141 tcagcgcggt agacgaaacg cggcaatgga tggtaatcaa accttccggc gtcgagtatg
    28201 acgtgatgac cgccgacgat atggtggtgg ttgagatagc cagcggcaag gtggtggaag
    28261 gtagcaaaaa gccctcgtcc gacacgccaa cgcatctggc gctttaccgt cgctacgccg
    28321 aaatcggtgg cattgtgcat acccactcac gccaccatct ggtcgcaggc cgggctggat
    28381 ctccccgcct ggggcaccac gcacgctgat tatttctatg gtgccatccc ctgcacgcga
    28441 cagatgaccg cagaggagat taacggcgaa tatgaatatc agaccggcga agtgatcatt
    28501 gaaaccttcg aagaacgtgg caggagtccg gcacaaatcc cggcggtgct ggtgcattct
    28561 cacggcccgt tcgcatgggg taaaaacgcc gccgatgccg tgcataacgc cgtagtactc
    28621 gaagaatgcg cctatatggg cctattctcg cgccagcttg cgccgcagct ccctgcgatg
    28681 caaaacgaac tgctggataa gcactacctg cgtaagcatg gggccaatgc ctattacggg
    28741 cagtaatccc tcacgccggg gcttcatcgc cccggcacta cgaattgata tgttccttgc
    28801 tgtaacgccg cttccacgct gctggcgtta aaccggtgtg cttgcgaaaa atctgccgga
    28861 aatagccaac gtcattaaac cgtattgcat ggagcaggcc gacaacgtcg agtcactgca
    28921 taacctgttg ctgcccgccg atgcgccatt tacgacattt gaaggcaagg gattgttcag
    28981 ccataagatc ctgattcaga cgccaggttc ccggtatgaa cagagcaatc cagagcaaca
    29041 acctttgtgg cattacgacg gagcaccagc cgcaccgaca tccaccggtg aattaaaaac
    29101 gccagctcac gccggtgata aaggtaaaac tctcattacg atcaatcatc gggctgttgg
    29161 taatctcttc cggcagctcg ctgtatcctg cgctcgccat caatacgacg tgctctccta
    29221 tcgggtattt tgccgtcagg ctgacatagg gcacccaggc atcctgcgcg gaataacttg
    29281 ccagaccgct acgacgggac tcactctctg aaatgccata gtaatagtca ctgaaattct
    29341 cgtcataata gagaacgccc agcgcaggtg tcagcgacag gcgacctatc tgcattttgt
    29401 ggaataccga tagctccccc acccagccgt tgctgttatc cagaacgtcc gcagccgcag
    29461 aggctttcac ggttccccaa cgctcgtggt gataccaggc aacccccgcc atagccgtac
    29521 tatcacgctt atcaagctgt tgcatggcat aatcgtcatt atcggcaggg tcaaattcca
    29581 gcggcatcca ggatgcggtc aggctaagtt catttttttc actttgcgac agaataaaac
    29641 ctaatgtggt ctgacgaaca taaaaagaat caccttcata actaatcagc ggaattgcct
    29701 ttgtattttc attataaccg cgataaggcg attcattata agctgcacca gcaccaatgg
    29761 ataattcaga agcagttgcg cttgccataa aaggcaacgc aaataacgcc acaatattgc
    29821 gattaattaa catgtccggt attccatttt
//
LOCUS       DAZGTR010000051        29799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGTR010000051 DAZGTR010000000
VERSION     DAZGTR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.051"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..950)
                     /gene="rspB"
                     /locus_tag="ACHLCY_002455"
     CDS             complement(<1..950)
                     /gene="rspB"
                     /locus_tag="ACHLCY_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFP0369698.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVA"
     gene            complement(962..2176)
                     /gene="rspA"
                     /locus_tag="ACHLCY_002456"
     CDS             complement(962..2176)
                     /gene="rspA"
                     /locus_tag="ACHLCY_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP0369699.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(2382..2708)
                     /gene="ynfA"
                     /locus_tag="ACHLCY_002457"
     CDS             complement(2382..2708)
                     /gene="ynfA"
                     /locus_tag="ACHLCY_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP0369700.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            2843..3184
                     /gene="ynfB"
                     /locus_tag="ACHLCY_002458"
     CDS             2843..3184
                     /gene="ynfB"
                     /locus_tag="ACHLCY_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP0369701.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            3219..3779
                     /gene="speG"
                     /locus_tag="ACHLCY_002459"
     CDS             3219..3779
                     /gene="speG"
                     /locus_tag="ACHLCY_002459"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP0369702.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(3782..4492)
                     /gene="ynfC"
                     /locus_tag="ACHLCY_002460"
     CDS             complement(3782..4492)
                     /gene="ynfC"
                     /locus_tag="ACHLCY_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP0369703.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            4600..4905
                     /gene="ynfD"
                     /locus_tag="ACHLCY_002461"
     CDS             4600..4905
                     /gene="ynfD"
                     /locus_tag="ACHLCY_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP0369704.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            5104..7530
                     /gene="ynfE"
                     /locus_tag="ACHLCY_002462"
     CDS             5104..7530
                     /gene="ynfE"
                     /locus_tag="ACHLCY_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP0369705.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            7591..10014
                     /gene="ynfF"
                     /locus_tag="ACHLCY_002463"
     CDS             7591..10014
                     /gene="ynfF"
                     /locus_tag="ACHLCY_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP0369706.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWVKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            10025..10642
                     /gene="ynfG"
                     /locus_tag="ACHLCY_002464"
     CDS             10025..10642
                     /gene="ynfG"
                     /locus_tag="ACHLCY_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP0369707.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            10644..11498
                     /gene="ynfH"
                     /locus_tag="ACHLCY_002465"
     CDS             10644..11498
                     /gene="ynfH"
                     /locus_tag="ACHLCY_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP0369708.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            11541..12155
                     /gene="dmsD"
                     /locus_tag="ACHLCY_002466"
     CDS             11541..12155
                     /gene="dmsD"
                     /locus_tag="ACHLCY_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP0369709.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            12314..13606
                     /gene="clcB"
                     /locus_tag="ACHLCY_002467"
     CDS             12314..13606
                     /gene="clcB"
                     /locus_tag="ACHLCY_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP0369710.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TREYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13559..14254)
                     /gene="bioD"
                     /locus_tag="ACHLCY_002468"
     CDS             complement(13559..14254)
                     /gene="bioD"
                     /locus_tag="ACHLCY_002468"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0369711.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(14379..15599)
                     /gene="mlc"
                     /locus_tag="ACHLCY_002469"
                     /gene_synonym="dgsA"
     CDS             complement(14379..15599)
                     /gene="mlc"
                     /locus_tag="ACHLCY_002469"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP0369712.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(15734..16627)
                     /gene="ynfL"
                     /locus_tag="ACHLCY_002470"
     CDS             complement(15734..16627)
                     /gene="ynfL"
                     /locus_tag="ACHLCY_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0369713.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRYTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            16734..17987
                     /gene="ynfM"
                     /locus_tag="ACHLCY_002471"
     CDS             16734..17987
                     /gene="ynfM"
                     /locus_tag="ACHLCY_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0369714.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(18036..18155)
                     /locus_tag="ACHLCY_002472"
     CDS             complement(18036..18155)
                     /locus_tag="ACHLCY_002472"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP0369715.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            18411..18719
                     /gene="asr"
                     /locus_tag="ACHLCY_002473"
     CDS             18411..18719
                     /gene="asr"
                     /locus_tag="ACHLCY_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP0369716.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            18812..18895
                     /locus_tag="ACHLCY_002474"
     CDS             18812..18895
                     /locus_tag="ACHLCY_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369717.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            18995..19816
                     /gene="ydgD"
                     /locus_tag="ACHLCY_002475"
     CDS             18995..19816
                     /gene="ydgD"
                     /locus_tag="ACHLCY_002475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP0369718.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(19855..20184)
                     /gene="mdtI"
                     /locus_tag="ACHLCY_002476"
     CDS             complement(19855..20184)
                     /gene="mdtI"
                     /locus_tag="ACHLCY_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP0369719.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(20171..20536)
                     /gene="mdtJ"
                     /locus_tag="ACHLCY_002477"
     CDS             complement(20171..20536)
                     /gene="mdtJ"
                     /locus_tag="ACHLCY_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP0369720.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            20948..21097
                     /locus_tag="ACHLCY_002478"
                     /pseudo
     CDS             20948..21097
                     /locus_tag="ACHLCY_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(21850..22131)
                     /locus_tag="ACHLCY_002479"
     CDS             complement(21850..22131)
                     /locus_tag="ACHLCY_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369721.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(22145..23806)
                     /locus_tag="ACHLCY_002480"
     CDS             complement(22145..23806)
                     /locus_tag="ACHLCY_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0369722.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VDRFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(23790..24146)
                     /locus_tag="ACHLCY_002481"
     CDS             complement(23790..24146)
                     /locus_tag="ACHLCY_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP0369723.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(24270..24452)
                     /locus_tag="ACHLCY_002482"
     CDS             complement(24270..24452)
                     /locus_tag="ACHLCY_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369724.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(24436..24876)
                     /locus_tag="ACHLCY_002483"
     CDS             complement(24436..24876)
                     /locus_tag="ACHLCY_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0369725.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(24876..25172)
                     /locus_tag="ACHLCY_002484"
     CDS             complement(24876..25172)
                     /locus_tag="ACHLCY_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0369726.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(25169..25507)
                     /locus_tag="ACHLCY_002485"
     CDS             complement(25169..25507)
                     /locus_tag="ACHLCY_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0369727.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(25504..26715)
                     /locus_tag="ACHLCY_002486"
     CDS             complement(25504..26715)
                     /locus_tag="ACHLCY_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0369728.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(26717..27289)
                     /locus_tag="ACHLCY_002487"
     CDS             complement(26717..27289)
                     /locus_tag="ACHLCY_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP0369729.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWVDLAGV"
     gene            complement(27329..28486)
                     /locus_tag="ACHLCY_002488"
     CDS             complement(27329..28486)
                     /locus_tag="ACHLCY_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0369730.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRHYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLTDESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDNFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(28774..29046)
                     /locus_tag="ACHLCY_002489"
     CDS             complement(28774..29046)
                     /locus_tag="ACHLCY_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0369731.1"
                     /translation="MVHDRIAEELEAKGFYRRASARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRPPEPPDNYGDLKKAVDRTYAEMGMDGAGNEIWRNYQDR"
     gene            complement(29059..29469)
                     /locus_tag="ACHLCY_002490"
     CDS             complement(29059..29469)
                     /locus_tag="ACHLCY_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0369732.1"
                     /translation="MTAQIAAYGRLVDDPLVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            complement(29479..>29799)
                     /locus_tag="ACHLCY_002491"
     CDS             complement(29479..>29799)
                     /locus_tag="ACHLCY_002491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369733.1"
                     /translation="RRGGIMSEPKFGEKLYKHNGRITIQQISAATPGWWVETDEGSSP
                     VASWALCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNA
                     GSLH"
BASE COUNT         7165 a         7871 c         7206 g         7557 t
ORIGIN      
        1 gcaacatgct ggaaatcaaa cgtatgggta attaattttt ctggtttaat taacccttta
       61 cttaaccagt cgataacaac cggaaattta tttgcattta agcgtgaaga gaaaatagag
      121 agttcttttc cggtaattcc ttgctgaatc acttcagacg gttcactgga gaagcccatc
      181 aatacaatac gtgccgctgg agaagccagc gttacggcct ctttcaggat agaaggatga
      241 caagccgcat cgataattaa tgtcggcttg atgccttttt cagcgaaaat ctcgccaagc
      301 ggtgtctggc tgttattaat cgcccagtct gccccgctct ctttcgcttt ttccagtcgt
      361 tcatcaatgc gatcggcaac aatcacattt ttaacgttat agacgccttt taatacctga
      421 acgatcgtca ggccgattgg accggcacca taaaccagaa cggtatcatt ttcagtcggt
      481 tgaccatgac cggtaacgtt agccgcaatg gtaaaaggtt cgatcatcac cgcatattga
      541 tcggccactg cttcaggaat tttccacgca ttttttgccg gaaccacggc atattcactg
      601 aaaccaccgt cagcgtgcac acctaataca gcaagtgtcg tacaaacgtt cggcttacct
      661 atagagcacg gatagcaatg cccacagctg accaccggat cgacagcaac gcgttcaccg
      721 actctggcgc tttccacacc ttcacccacc gcatcaatga cgccaaagaa ttcatgacca
      781 atgacgcgcg gatatttcgc aaaaggatta tgcccacggt aaatatggct atctgaacca
      841 caaattccgg caagtttcac ttttactcgt acttcacccg ctgacggggt gggtatttca
      901 cgttcgataa tcgacagttg attcggtttt tcaattaaaa tgcttttcat taccttactc
      961 cttaccagtt ccacagcgtg ccatcttcca gacgtgcgac tggcagataa gcaggttcat
     1021 agggatattt cgccgccagc ttttcatcga attcgatgcc aagacccggt ttgtctcccg
     1081 gatgcatata gccgttatcg aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg
     1141 aataacccat gtactcctgg acaccgaaat tggggaccca cagatcaaag tgcagcgccg
     1201 cagccatgca gactggtgac aaatcggaag gaccgtgtga gccagtacgt acctgataca
     1261 gcgaagcaaa atcggcaatc cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg
     1321 tgcggatata atcgatgagt tgctcttcaa tcagttgttt gcagtcccag atgctgttga
     1381 agacttcacc cactgcgatg ggtgtgacgg tatgttggcg aatgagacgg aagcattcct
     1441 ggttttccgc aggcgtcggg tcttccatcc agaacatgcg ataatcttca atgcttttac
     1501 caaagcgcgc cgcttcaata ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat
     1561 taaaaccaaa cttgttacgt accgcgtcaa acaatttcgg catgaaatcg aggtatttct
     1621 ccgtcgacca cagctgctct tccggccact gtcctttggt tgcgggttca taagccagac
     1681 ctttaccttt cgacatgccg taggtggttt tcataccagg gattccgcac tgcacgcgga
     1741 tggctttgaa tcccagctcc tgatgacggg cataatcatc cagagcttca tcaatactgt
     1801 gaccggtggt atggcaataa accatcaccc cttcacgaga cgcgccgccg agtaactggt
     1861 aaagcggcat gttggcagct ttggctttaa tatcccacag cgccatatca accgctgaaa
     1921 tggccgacat cgtaaccgga ccgcgacgcc agtaagcacc tttatagaaa aactgccaga
     1981 tatcttcgat acggtgcgca tcgcgaccaa taagctgcgg acaaaggtga tcctgcaaat
     2041 aagaggccac ggaaagctca cgtccattga gggtggcatc cccaaggccc gtaataccgt
     2101 cctcagtggt gatttttaat gtgacgaaat tacgccccgg acaggtaaca aaaacttcag
     2161 cctttacgat cttcatgttc gattccttgc atcgcttgtc gtgatgcatg aaatctacgc
     2221 aactgagcta ctaccataca agtataaaga tcgaaaaaag ccggagtgat cacaaaaaaa
     2281 ggcgtatatt tgcgctgtga atggttgaca aaagatgaaa tagaatacct tttgtcagct
     2341 gacacttcct cttatcttat tgataaaatg gatttatgtt cctacgtgcg cccccagccc
     2401 gcaacaatga tcaacatgcc gcaaagcgca atcaacgcac ccgtccagtc ataaagagtc
     2461 agtttcacgc catccacaac gcgcagccac atcaacgccg tgcagacata aacgccacca
     2521 taagccgcgt aaacacgccc actcgccgct ggatgcaacg ttaacaacca gacaaacagc
     2581 gccagtgaaa tccccgccgg aagcaacagc cagatactgg cgtttcgttt taaccacaac
     2641 cagggcagaa agcatccaat aatttcacac agcgcagtag caaaaaatag taacgttgtt
     2701 ttaatcatct ttgtctctta ttgacatcat gtatagttat agggcgacat aatatcatca
     2761 atataaacac cctcctggta cgttttgcgt ccgcagtgga tggtgtagaa tcacctttaa
     2821 tcattcatac agggaatgaa ttatgaaaat cactctcagc aaacgaatcg gcctgctcgc
     2881 tattctgctg ccttgcgcac tggcattgag cacaactgtt catgccgaaa ctaacaaact
     2941 ggtgattgag tctggcgaca gtgcacaaag ccgccagcac gccgctatgg aaaaagagca
     3001 atggaatgac acgcgcaatc tgcgccagaa agtgaataaa cgcactgaaa aagagtggga
     3061 taaagccgac gccgcttttg ataaccgcga taaatgtgag caaagcgcca acatcaatgc
     3121 ctactgggag cccaatactt tgcgctgcct ggaccgtcga actggccgcg ttattacccc
     3181 ctaacctgtt attgatttaa ggaatgtaag gacacgttat gccaagcgcc cacagtgtta
     3241 agctacgccc gctggagcgt gaagatttac gctatgtaca tcaactcgac aacaacgcca
     3301 gtgtgatgcg ttactggttt gaggaaccct acgaagcctt tgttgaactc tctgatctgt
     3361 atgataagca tattcacgat cagagcgaac ggcgctttgt ggtggaatgt gacggcgaaa
     3421 aagccggtct ggtggagctg gtggaaatta accatgttca tcgccgcgca gaatttcaga
     3481 taattatctc cccggagtat caggggaaag gtctggcaac ccgtgccgcc aaattagcaa
     3541 tggactatgg ctttaccgtt ctcaatctct ataagctgta tctgatcgtt gataaagaga
     3601 atgaaaaagc gattcacatt taccgcaagc ttggcttttc ggttgaaggt gaattgatgc
     3661 acgagttctt tattaatggt caatatcgta atgccattcg catgtgtata ttccagcatc
     3721 agtatctggc agagcacaaa acaccgggtc agactctcct gaagccgacc gcacaatagc
     3781 attaataata atcgatcgta tttttgatgg tgtaaacccg ttcgacggcg ggttttactc
     3841 cttcatcaac aatgattagc tgacagtcca ccggattagc gtgactgtca tattcacagc
     3901 tctgttttac attaccaacc cgttgattat tcagtaaagt aaccgctgtg taatctaatt
     3961 ttttgatcgg atccgttgat ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg
     4021 tggttttacc cagcggataa ccctgatcat catagcgata ttccatctgc atttgtttgc
     4081 tgctggcttt aatcacgaag ccattatcat cggtttccca actcaccccg gcagaaggta
     4141 attctgctag ctggcatttt ccctgtaaac gtactctctt ctccagcgtc tcggcatcac
     4201 ggtaataatt ggcgtccagt accagagcga ccacggtatt attttccaga tccagtaatt
     4261 cgagtgaatc aaaacagcct tcttccgaca aagtcccaga aacacgtttc gtcacttcac
     4321 cttgctcatc cattaatgtc tgagtgaaat cttttaccgg accacgcagc ggatcaaaat
     4381 cgaattcatt agagaaactg gccatctcag gggtaaatga aagtgttacc tctgtgcggt
     4441 cacatcctgt gaggaatatc gcgagtaagc atggtagtaa tttgtatttc acaacagtca
     4501 ccagagagta gagatgattc tcaatcatag tagcaaatac agtactttac acgttaaatg
     4561 ctatgcttaa agaagttatc ttcgcgtaag gagcttatga tgaaactctc aacctgctgt
     4621 gccgcgcttc tgctcgccct cgcctcaccc gtggtactcg ccgcgccggg atcctgtgag
     4681 cgcatacaga gcgatatatc acagcgcatt atcaataatg gcgtacccga aagcagcttc
     4741 acgttaagta ttgtacccaa tgaccaggtt gatcagcctg attcccaggt cgtcggccat
     4801 tgcgctaatg atacgcataa aattctctat acccgcacca ccagcggtaa cgtctctgct
     4861 cccgcgcagt ctacccagga tggcgcgcct gccgaaccgc agtaatacat tatcgactga
     4921 acgccggata cgacacatcc ggcgatttga acatacaaca taatcccacc ttattactca
     4981 tacccttcta ttgatatgga ttaataattc ttaacccaaa atgggtagac tccctctatt
     5041 gttagcgcgc taaatattca atatataaac atttatataa cgataaagaa caaggagtga
     5101 gttatgtcca aaaatgaccg aatggtgggc atcagccgca gaacactcgt taaatcaacc
     5161 gcgataggtt ctctggcgct ggctgcaggc ggtttttctt taccgttcac cctgcgcagt
     5221 gcagcggcaa cggtacaaca ggccagcgaa aaagtgatct ggggtgcctg ttccgtcaac
     5281 tgtggtagcc gctgtgcact tcgtctacat gttaaagata atgaagtgac ctgggtggaa
     5341 actgacaata ccggcagcga tgagtacggc aaccatcagg tacgcgcctg tttgcgcggt
     5401 cgctccatcc gccggcgtat taatcatccc gatcgcttga attacccaat gaaacgcgtg
     5461 ggtacacgcg gtgaaggcaa attcgaacgg attagctggg atgaagccct ggatactatc
     5521 gccagtagcc tgaaaaaaac cgtcgaacaa tatggcaatg aggctgtata tattcagtac
     5581 tcttcgggga ttgttggcgg caatatgacc cgctcttcgc catcagcctc ggcggtcaaa
     5641 cgcctgatga actgctacgg cggttcactc aatcagtatg gctcctacag caccgcgcaa
     5701 atttcctgtg ccatgcccta cacctacggc agtaatgatg gcaacagcac cacggatatt
     5761 gaaaacagca agctggtcgt gatgtttggt aacaacccgg cagaaacccg catgagcggc
     5821 ggtggcatca cttatcttct tgaaaaagcg cgcgagaaat cgaacgccaa aatgattgtt
     5881 atcgatccgc gatataccga tacggctgct ggtcgtgaag atgaatggct ccctattcgc
     5941 ccgggcaccg atgccgcgct ggttgcgggt attgcctggg tattgattaa cgaaaatctc
     6001 gttgatcaac cttttctcga taaatactgc gtcggctatg acgaaaaaac cttacccgca
     6061 gatgcaccca aaaatggtca ctataaagcc tatattcttg gtgaaggtga tgataacaca
     6121 gcgaaaacgc cgcagtgggc ttcgcaaatt accggtatcc cggtagaccg tatcatcaaa
     6181 ctggcgcgtg aaattggcac agcaaaaccc gcttatatct gccagggctg ggggccgcaa
     6241 cgccaggcaa acggcgaact gactgcacgc gctattgcta tgttacctat tttgacgggc
     6301 aatgtcggca tcagcggcgg aaatagtggc gcgcgtgaat cgacctatac cattaccata
     6361 gaacgcctgc cggtgctgga taatccggtc aaaacgtcaa tctcctgctt cagctggaca
     6421 gatgctatcg atcatggtcc gcaaatgacg gcaatccgcg acggcgtccg cggcaaagat
     6481 aaactggatg tgcccattaa gttcatctgg aactacgcgg gaaataccct cgttaatcag
     6541 cattctgaca tcaacaaaac gcatgaaatt ctgcaggacg aatcgaaatg cgaaatgatt
     6601 gtgatcatcg aaaactttat gacctcatcg gcaaaatatg ccgacattct gctgccagac
     6661 ctgatgaccg ttgagcagga agatattatt cctaacgact acgccggtaa catgggatat
     6721 ctcattttcc tccagcctgt caccagcgaa aaattcgaac gcaaaccgat ttactggatc
     6781 ctgagtgaag tcgcgaaacg tcttggacct gacgtctatc aaaagttcac agaaggtcgc
     6841 acgcaggaac aatggttaca acatctgtac gccaaaatgc ttgccaaaga tccggcgtta
     6901 ccgtcttacg acgaactgaa aaaaatgggt atctataagc gtaaagatcc caatggccat
     6961 tttgtcgcct acaaagcatt tcgtgacgac cccgaggcaa atccacttaa aacgccttcc
     7021 ggtaagattg aaatttattc cagcaggctg gcggaaattg cccgtacctg ggaactggaa
     7081 aaagatgaag tgataagccc attgccagtt tatgcctcaa cctttgaagg ctggaactcc
     7141 cctgaacgta gaaccttccc actgcaactg tttggtttcc attacaaatc ccgtactcac
     7201 tcgacctacg gcaatattga tctcctgaag gctgcctgcc gtcaggaggt gtggatcaac
     7261 cctatagatg cgcagaaacg tgggattgcc aacggcgata tggtgcgggt gtttaaccat
     7321 cgtggcgaag ttcggctacc agccaaagta acaccacgta ttctccctgg agttagcgct
     7381 atgggccagg gagcctggca cgaggccaat atgtctggcg ataaaatcga ccatggcggc
     7441 tgtgtgaata cgctaaccac tctgcgccct tcaccactgg cgaagggaaa cccgcagcac
     7501 actaatctgg tcgagatcga aaaaatataa cccacgacaa ccataaattc tggcatgaca
     7561 ttttgtttga aaagcaataa gtgagtaatg atgaaaatcc ataccacaga ggcgctcatg
     7621 aaggctgaaa tcagccgtag aagtctgatg aaaacctccg cacttggcag tcttgcgctg
     7681 gcaagcagtg ctttcactct gccattttcc caaatggtcc gggcggcaga ggctccggta
     7741 gaagagaaag cggtctggag ttcctgcacc gttaactgcg ggagccgctg tctgttacgt
     7801 ttgcatgtga aagatgacac cgtgtactgg gtggagtctg atacgacagg tgacgacgtc
     7861 tacggtaacc atcaggttcg agcgtgttta cgcgggcgct ctatccgccg acggatgaat
     7921 catcctgata ggttgaaata tcccatgaag cgcgtcggca agcgcggtga aggtaaattt
     7981 gaacggataa gttgggacga agccctggat accatcagtg ataatcttcg gcggatcctg
     8041 aaagattacg gcaatgaggc tgtacatgtc ctgtacggaa caggcgtaga tggcggaaac
     8101 atcaccaact caaacgtccc gtaccgtctg atgaactctt gcggtggttt tctcagtcgc
     8161 tatggcagct acagtaccgc acagatcagt gccgcaatga gttatatgtt cggtgccaat
     8221 gatggcaaca gcccggatga tatcgccaat acgaaactgg tcgttatgtt cggaaataac
     8281 ccggcagaaa cgcggatgag cggcggtggt gtcacttact acgtcgagca agcccgcgaa
     8341 cgttcaaacg cacgcatgat cgtcatcgat ccacgttata acgacactgc tgccgggcgt
     8401 gaagatgaat ggctgcccat tcgccctggc accgatggcg cactggcctg tgcgattgcc
     8461 tgggtactga ttactgaaaa catggtcgat cagccatttc tcgacaaata ttgtgttggt
     8521 tacgatgaaa aaacgctgcc cgccaacgca ccacgtaacg cgcattataa agcctatatt
     8581 ctgggcgaag ggcctgacgg catagctaaa acgccggaat gggcagcaaa aatcaccagc
     8641 atcccggcag aaaaaattat ccagttggca cgagagatcg gttcagcaaa acctgcttat
     8701 atttgtcagg gttgggggcc acaacgacat tccaacggcg agcaaacgtc ccgcgctatt
     8761 gccatgcttt ccgttctcac cggtaacgtc ggcataaacg gcggcaactc aggtgtacgc
     8821 gaaggtagct gggatctggg ggtagaatgg ttcccgatgc ttgagaatcc tgttaaaacg
     8881 cagatttccg tctttacatg gacagatgcc atcgaccatg gtacggaaat gaccgcgacc
     8941 cgtgatggtg ttcgtggaaa agaaaaactg gatgtcccca tcaagttttt atggtgctac
     9001 gccagtaaca cattgatcaa tcaacatggc gacatcaatc acacccatga ggtgcttcag
     9061 gatgacagca agtgcgagat gattgttggc attgaccact tcatgacggc ctcggctaag
     9121 tattgcgata tcctgttgcc cgacctgatg ccaacagagc aagaagacct tatctctcat
     9181 gaatctgcag ggaatatggg ctatgtgatc ctcgcccaac ccgcaacctc agcaaaattt
     9241 gaacgcaaac ccatctactg gatgctgagt gaagtcgcca aacgcttagg accagacgtt
     9301 tatcaaacct ttactgaagg tcgcagtcag catgaatggg tcaaatatct ccatgcgaaa
     9361 acgaaggaac gtaaccctga gatgcccgac tacgaggaga tgaaaacgac cgggatcttt
     9421 aagaaaaaat gcccggaaga acactacgtc gctttccgcg cattccgtga agatccacag
     9481 gcaaacccgt tgaaaacacc ttcggggaaa atcgaaattt attctgaacg actggcgaag
     9541 attgcagata cctgggaatt gaaaaaagat gaaattattc atccccttcc tgcgtatacc
     9601 ccaggttttg atggctggga cgatcccctg cggaaaacct atccactgca gttaacgggc
     9661 ttccattaca aagcgcgtac ccactccagc tacggcaata ttgatgtgtt acagcaggcc
     9721 tgcccacaag aggtgtggat caaccccatt gatgctcagg cacgcggtat ccgtcatggc
     9781 gataccgtgc gggtatttaa caataatgga gaaatgctga ttgccgcaaa agtgactccg
     9841 cgtattctgc ctggcgttac cgccatcggg caaggtgcgt ggcttaaagc ggatatgttt
     9901 ggtgaccggg tcgatcacgg cggcagtatc aatattctga cctctcaccg cccttcaccg
     9961 ctggcaaagg gaaacccgtc gcacagcaat cttgtccaga tcgaaaaggt ttaaggagta
    10021 gcccatgacc acacaatatg gattttttat cgattccagc cgctgtactg gctgtaaaac
    10081 ctgcgaactg gcgtgcaaag attttaaaga tcttggcccg gaagtcagtt tccgccgtat
    10141 ttatgaatac gctggcggcg actggcagga agataatggc gtctggcacc agaacgtgtt
    10201 cgcttattat ctctccatct cctgtaacca ttgcgatgac cccgcatgta caaaagtctg
    10261 ccccagcggc gcaatgcata agcgtgaaga tggctttgtg gtggttgatg aagatgtttg
    10321 tatcggctgc cgctactgcc acatggcttg tccgtacggc gcaccacagt acaatgctga
    10381 aaaagggcac atgacgaagt gcgatggttg ttattcgcgc gtcgccgagg ggaaacaacc
    10441 catatgcgtc gaatcctgcc cgctgcgtgc acttgagttc ggtccaattg aagagctgcg
    10501 ccagaagcac ggcactctgg ctgccgtcgc gccactgccg cgcgcgcatt tcacaaaacc
    10561 caatatcgtt atcaaaccta acgccaacag ccgcccgacc ggcgatacca ctggttatct
    10621 ggctaatccg gaggaagtgt aaaatgggaa atggatggca tgaatggcca ctggttatct
    10681 ttaccgtatt gggtcaatgc gtagtaggcg cgctgattgt tagcggaatt ggctggttcg
    10741 ccgccaaaaa tgacgcagac cgtcagcgta ttgtccgtgg catgtttttc ctctggctgt
    10801 tgatgggcat cgggtttatt gcctctgtca tgcacctggg atcgccactg cgggcgttta
    10861 actcgctgaa tcgaatcgga gcttctggtc tgagtaacga gattgctgcc ggttccatat
    10921 tttttgccgt tggcggacta tggtggctgg tggcggtcat cggtaaaatg ccgcaagcgt
    10981 tggggaaact ctggttactt gtcagcatgg cgctcggcgt cattttcgtc tggatgatga
    11041 cctgcgtgta tcaaatcgac actgtgccaa cctggcataa cggttacact acgctggctt
    11101 tcttcctgac ggtattgttg agcggtccaa tacttgctgc cgcaattctg cgggcagccc
    11161 gcgttacatt taataccaca ccatttgcca tcattagcgt tctggcgctg atcgcttgtg
    11221 ccggggtgat tgtgctgcaa ggtttgtctc tggcgtctat tcactcatcc gtgcaacaag
    11281 ccagcgcact ggtaccagat tacgcctcct tacaggtctg gcgtgtggta ttgttatgcg
    11341 caggtcttgg atgctggctt tgcccgctca ttcgtcgccg tgaacctcac gttgccggac
    11401 ttattctggg gctaatcctc atacttggcg gtgagatgat cgggcgtgtg ctcttttatg
    11461 gcctgcacat gacagtagga atggctattg caggttaaca aattggtgcg gggcaacccg
    11521 cacatttcag gatgttagga atgacccatt tttcacagca agataatttt tctgtcgcgg
    11581 cgcgcgtgtt gggcgcgctg ttttattacg ctccagagag tgcggaagcc gcacctctgg
    11641 ttgcggtact caccagtgac ggctgggaaa ctcagtggcc tctaccagaa gcgtcattag
    11701 cgccgctggt gacggctttt cagacacagt gtgaagagac tcacgcccag gcctggcagc
    11761 gtttgtttgt cggcccgtgg gcactgccgt ctccgccgtg ggggtcggtc tggctggatc
    11821 gcgaatctgt gctgtttggc gattcaacat tggcacttcg tcagtggatg cgcgagaaag
    11881 gcattcagtt tgaaatgaag caaaacgaac cggaagatca ttttggttca ctgctgttga
    11941 tggctgcgtg gttggcggag aatggtcgcc agacagaatg cgaagaattg ctggcatggc
    12001 acctttttcc gtggtcaaca cgttttcttg atgtttttat cgaaaaagca gaacaccctt
    12061 tctaccgtgc actgggtgaa ctggctcgct taacgctggc gcaatggcaa tcacaactgt
    12121 taattcctgt cgcggttaaa ccgctgtttc gataggatca ccgtaatatt gccggatggt
    12181 gacgattacg cgcaatccgg caataatagg ttacagtgtc acgttttttt atctcttaaa
    12241 gcacgcactg cttttgcggc tggcctcttt tgccgcaaaa tagtcgcccg tgtttcattg
    12301 cccatttctg ctcatgcatc atctacacat ctatccggat ctgcgcacta tgttccgccg
    12361 tctgcttatc gcaacagtcg tcggtattct cgcggccttt gccgttgccg ggtttcgtca
    12421 tgcgatgctg ctactggagt ggttgttcct caataatgac tccggcagtc tggtcaatgc
    12481 agcgacaaac ctttccccct ggcgacggtt gctaactccg gcgctcggcg gactggcggc
    12541 gggtttgttg ctgatgggct ggcagaaatt tacccaacaa cgccctcatg cgccgaccga
    12601 ttacatggaa gcgttgcaaa ccgatggaca gttcgattac gcagcaagcc tggttaaatc
    12661 gcttgcctct ctgctggtag taaccagcgg cagtgcaatt ggtcgcgaag gtgcgatgat
    12721 tcttttagct gcccttgccg cctcctgttt tgcccaacgt tttacgccac gccaggagtg
    12781 gaaattatgg atcgcctgtg gggccgcggc gggaatggct gcggcctatc gtgccccgct
    12841 tgctggcagt ttatttatag ccgaagtgct gtttggcact atgatgttgg cctctctcgg
    12901 cccggtgatt atttccgccg tcgtggcatt gctggttagc aatctgatta atcatagcga
    12961 cgcgttactc tacaacgtac aactctcagt gacggttcag gctcgtgact atgcgctgat
    13021 tatcagtaca ggtgtgctgg caggtctgtg cggaccactg ttgttaacgt taatgaacgc
    13081 ctgtcatcgt ggatttgtga gtctcaaact tgcgccgccc tggcaactgg cactaggcgg
    13141 gttgatcgtg ggtctgcttt ccctgttcac acctgcagtg tggggcaacg gctatagcac
    13201 cgtacaatcc tttttaaccg ccccaccact gttaatgatc attgccggga tcttcctttg
    13261 taaactgtgt gccgtgctgg cgagtagcgg ttccggcgca cccggtggtg tctttacacc
    13321 gacgctattt atcggtcttg ccattggcat gttgtatggt cgtagcctgg gattatggtt
    13381 ccctgatggc gaagaaatta cgcttttact cggattgacc gggatggcga cactgttggc
    13441 ggcaaccacg cacgcgccga ttatgtcgac gttgatgata tgtgaaatga ccagggagta
    13501 tcagctactc cccggtttat tgattgcctg cgtcattgcg tcggtaattt cgcggacgtt
    13561 acaccgtgac tctatctacc gccagcacac tgcgcagcat agctaagcgg atgtattgcc
    13621 ccagttctcg ctgttcggcg cgcggcagat aaggcagttc accaatgagc ggtgccggaa
    13681 gttttttacc cagcacatca atgatttccg cataatgcgc cagtcctggg ttgattcggt
    13741 tagccaccca gccaatgagc ggcagcccgt cgttggcaat cgcctgagct gttagcagtg
    13801 catggttaat gcaaccttcc tgaataccga caaccatcaa caccggcaac tgttcctgca
    13861 ccacccattc agagagtgga cgcaaatcat tcatcagact gcgccagccg ccagtccctt
    13921 ctaccacgac atgatcgact ttatcggtca ggtttgccag gccgtttgaa atgagggtgt
    13981 aattgattgg gcaactgtgc gccacgctac tttcttcttc gcttaacgcg ataggattaa
    14041 ctgcttcata aggcagttcg atggttgaaa cactctgcaa caccagggca tctttattac
    14101 gcagcccttc gggtgtctct ttgctcccct tcgctacggg tttatatccc gcaaccgttt
    14161 ttccctggga ggctaacgct tgtagcaatg cgcgggaaac caccgttttc cctacagaag
    14221 tgtctgtacc ggtaataaag aaacgcttca gcatcactaa ctccaccgtt atgcttcaca
    14281 aatataaacc aggaaaataa ttaaccttga aagtctaagt tatgctttcc tggcccaaat
    14341 tgagatagcg caaattttgg tagaacagtt aaaaaatgtt aaccctgcaa cagacgaatc
    14401 aacaaagaac cgttatacat cgcgtctttt accagtgcag cgcctgccat cgtgccctgg
    14461 ttagaaaact gagtactctc aacgctgatg tgctgactat acgcaggaag ggcctgctga
    14521 cggatgctgt ctgagatgac cgggaagagg atatctgccg ctttacttaa cggtgagcca
    14581 atcagtattt tttgtgggtt aaataaattc accatgatgg caagaatgcg ccctacatgc
    14641 gcgcccaccc cggtaatgat gtcttttgcc agcagatcgc cgcgcaatgc cgcctgacac
    14701 aatgagtcca cggttaacgg ttgtccatgt aacatcgagc tcatggattg attaagacgc
    14761 agctgtgcca gctcaagaat actgtccaca ctggcgatgg tttcgaggca gccgtgattc
    14821 ccgcaataac agcgtttccc atacgggtcg acctgtgtgt ggcctatttc cacgagacta
    14881 ctgctgcctg cgtgtagcag atgaccatcg gtaatgacgc ccgcccccac gttgtgatcg
    14941 ataaccacct gaatcacatc gcgcgccccg cgtgaggcac caaacaaggc ctctgccatc
    15001 gtccatgcgc tgatatcatg ctgaatataa accggtacgc cggtatgctg ctccagcgcc
    15061 tcgccgagcg gcatctcttt tacatcctcg tagaacggca tgcgatgtac aataccattt
    15121 tccgtatcaa taattcccgg caaggttatg gcaatcgaag ttagacgctc aagttttttc
    15181 tggtggcgaa taaaaaactg atcgatatgg gaaatgatac gatccagcaa tggcgagtca
    15241 tcttttaacg ccagttcctg cgactcttcc accaccagtt tgctgctcag atcgcgcaga
    15301 gcaaggaaaa tctccccgcg actaatgcgc agagaaagat agtgccaggc ttcagtttca
    15361 accaccagcc ccaccgccgg acggccacgg ttccccgctt ctttgatttc cagctcttgc
    15421 accaggtgtg cttcgagcat ctcacggaca attttagtga tactggcagg agccagctgc
    15481 gccagacggg aaagatcgat acgcgagact ggaccaagct gatcaatcag gcgataaacc
    15541 gcgcccgcgt tggtctgctt tatttgatca atgtgcccag gctggttttc agcaaccacc
    15601 gcatactccc tatattttcg cgctccgaaa taatctgtag gctatggtga agcacttcaa
    15661 tacgtgtcgt caaattttta cttaggcatg tgattaacag cacatttttc gggctttttc
    15721 gctgaaattt ccctcacctg agagcattca gcagatgaat acgaaagtta cgcgcagccg
    15781 gactttgttc atgatgtttc ggccagacca accacatttc agaaaccgca tcctcttcag
    15841 caatcggcac ccagcgcatt tcgttgagct gaacacgttt aaatgaagca ggcaaaattg
    15901 aaacacccag accggcggaa accagaccga tgatggtcat tgcctcgccc acctcctgag
    15961 tgatgacggg cgtcaaatgg taacgtcgca tcagcccgag aatatcgtca tacagccctg
    16021 tcccgacgtg cggatcaaaa aagacgaagg gttctttcgc cagttcagcc agcgttacat
    16081 tcgggttatt tgccagggga tgatcgtgcg ggatcatcgc cataagcggt tcatggacga
    16141 tgactgcgtg ttcaagcgtc tccggtaacg ctgtgtaacg cagcaatccc atatccagcg
    16201 ttccttcaat gagcggagcg atttgctcac gagtgttcat ttcgcgggtt tgtaaatgga
    16261 catcgggata atcacggcga aacagcgata acgtatcgga caccgcccga ataaaaggag
    16321 cggacgaagt aaaaccaatg cgcaactccc ccgcttcccc ctgatgcagt ctttcagcgc
    16381 gagcggcagc gtcatctacc atagacagga tttgccgact atctgcaaga aactgttttc
    16441 ctgctgccgt cagcaataca ctgcgattag ttcgtgccag cagtcgggca ccaatttgtt
    16501 gctccagtgc ctgaatctgc tgacttagcg gtggttgcga aatattcagg cgggcagcgg
    16561 cgcgcccgaa atgcagctct tccgcaacag caacaaagta acgcagatga cgaagttcaa
    16621 tattcatatt taaaacatct tatttgagat tattaatata ttagacagaa caattcgatt
    16681 ttcctaacct atgtataagc ctgatccaca ggcatattta gcaaggattt caagtgagcc
    16741 gtactacaac tgttgatggc gctccggcaa gcgacactga caagcaaagc atttctcagc
    16801 caaatcaatt tattaaacgc ggtacgccgc aatttatgcg cgtcaccctg gcgctgttct
    16861 ctgccggact ggcaacattt gcacttctct attgtgtgca gcctatcctt ccggtgcttt
    16921 cgcaggagtt tggcttaacg cccgcgaaca gtagtatttc actgtccatt tccacggcga
    16981 tgttggctat tggtttgctg tttactggcc ccctatccga tgccattggt cgcaaaccag
    17041 tgatggtcac ggcgctactg ttggcctcca tttgtacgtt actttcgaca atgatgacca
    17101 gctggcacgg cattttgatt atgcgcgcct tgattgggct ttcgttaagt ggcgtggcag
    17161 ctgttggcat gacttatctt agcgaggaaa tccaccccag tttcgtggcc ttttcgatgg
    17221 ggttgtatat cagcggcaac tcaattggcg gcatgagcgg acgcttaatt agtggtgtct
    17281 tcacggactt tttcaactgg cgaattgctc tggcggcaat cggttgtttc gcgctagcct
    17341 cggcgttgat gttctggaaa atcctccctg aatcacgcca ttttcgcccg acttcgctgc
    17401 gacctaagac gttgtttatc aactttcgtc tgcactggcg tgaccgggga ttaccgttat
    17461 tgttcgcaga aggctttttg ctgatggggt cgttcgtcac gctgtttaat tacatcggat
    17521 atcggttgat gctctccccc tggcatgtca gtcaggccgt ggttggctta ttatcgctgg
    17581 cttatttgac cggtacatgg agctcaccca aagccggaac catgaccacc cgctacgggc
    17641 gcggtccggt gatgttgttt tcgacggggg ttatgctgtt tggtttactg atgaccttat
    17701 tcagctcgct gtggctgatc tttgccggaa tgttactctt ctcagcagga ttcttcgcag
    17761 cccactcagt tgccagcagc tggattggcc cccgcgcaaa acgcgctaaa ggccaggcct
    17821 cctcgctgta tctgttcagt tactatctgg ggtcgagtat tgccgggacg ctgggtggtg
    17881 ttttctggca taactatggc tggaacggcg tcggcgcatt tattgctctg atgctggtca
    17941 ttgctctgct ggtcgggacg cgtttgcatc gtcgtctgca cgcctgaaaa ataagcccgg
    18001 actgcggtaa ctacacgtcc ggacttattg cttgctcaaa ccaacgttaa tagccatcct
    18061 aaaatagacg aagcgccagc caattcccgc agcgcgtcta gcgtcatcag gattataagt
    18121 acccaaataa acggattcat tttgctgtgt gtcatttatt actgatgcgc agttattcta
    18181 ccgctttgta agtagtaaaa tagttaaccc gatcaagact actattattg gtagctaaat
    18241 ttcccttaag tcacaatacg ttattatcaa cgctgtaatt tattcagcgt ttgtacatat
    18301 cgttacacgc tgaaaccaac cactcacgga agtctgccat tcccagggat atagttattt
    18361 caacggcccc gcagtggggt taaatgaaaa aacaaattga gggtatgaca atgaaaaaag
    18421 tattagctct ggttgttgcc gctgctatgg gtctgtcttc tgccgccttt gctgcagaga
    18481 ctgcgaccac acctgctccg actgcgacga ccaccaaagc agcgccggcg aaaacgacac
    18541 atcataaaaa acagcataaa gcagcacctg cccagaaagc gcaggcggct aaaaagcatc
    18601 ataaaaatac gaaagctgaa cagaaagccc ctgaacaaaa agcgcaggca gcgaagaaac
    18661 acgccgggaa acacagccat cagcaaccgg caaaacctgc tgcacaaccc gcagcgtaag
    18721 ttttcaacag taatgctggc gcgccccctc gcgcctgaaa attacggtgc taagcaggta
    18781 acgtttagca ccgcctttag cggagggtag tatggtgggc cgttatcgct ttgagttcat
    18841 tcttatcatc cttattttat gcgcactgat tgccgcccgt ttttatcttt cctgactgta
    18901 gttatctgat tttactccca ctctcatccc gtcccgtcta tagtatttat gagggtttgc
    18961 ttttaataat cataattacc caccagagtg tgatatgcgt acaaccattg ctgtagtgtt
    19021 gggtgcaatt agtttgacgt ctgcttttgt gtttgcagat aaaccagacg ttgccaaatc
    19081 ggcaaacgat gaggtcagca ccctgttttt tggtcatgat gatcgtgtgc cagtgaatga
    19141 cacgacccaa tcaccgtggg atgcggttgg gcaactggaa acggccagcg gcaatttatg
    19201 tacggcgacg ctgattgcac ccaatctggc attaacggca ggacactgtt tattgacgcc
    19261 gcctaagggt aaagcggata aggcggtagc gctgcgtttt gtgtcaaata aaggtctttg
    19321 gcgctatgac atccacgaca tagaaggccg cgttgatccg acactgggaa agcggttaaa
    19381 agcagatggg gatggttgga ttgttcctcc cgcagctgca ccatgggact tcggcttgat
    19441 tgtgctgcgt aatccccctt ctggcattac accgttgccg ttatttgagg gagataaagc
    19501 cgcgcttact gccgcattaa aatcggctgg tcgtaaagtg actcaggcgg gatatcctga
    19561 agatcatctc gatacgttat acagtcatca aaactgtgaa gtgactggct gggcgcaaac
    19621 gtcggtgatg tcacatcagt gcgatacctt gccgggtgac agcggttcgc ctttgatgtt
    19681 gcataccgat gacggctggc aattaattgg ggtgcaaagt tcggctcctg ccgcgaaaga
    19741 tcgctggcgc gccgataacc gggccatttc tgttaccggt tttcgcgaca agctggatca
    19801 actgtcgcaa aaataatgtt caaacgctgc ccgacagtgc gggcagcgtg ttcatcaggc
    19861 aagtttcacc atgatcattc cagccaacag caagaccagg ccaatccagc ctttacgatt
    19921 taaccgttga ccaaacaaga tccaacctgc ggctaacgtg gcggcaatac caaacccgcc
    19981 ccacaatgca taagcgacag acaagtcgat cccttttacg gcctgagaaa gcgcactaaa
    20041 ggcagccagc accgccgcca gggagagcaa gccaaatatt ttgcgacgaa agccgtcaga
    20101 aaatttcaaa aagacgttag caacgatttc cagcacgatt gccaatgcca gccaggcggc
    20161 gtgaacccat tcaaactgcg ccatggttca cctccagttc aggtttacgc gctttacggg
    20221 tacctgattt gatcaacaca atcccggcga ccagggtggt taacccggca attttcatca
    20281 gcgataaact ttcgtcgaat aacaaaacgc taaacaaggt aataaataaa ataccgatac
    20341 cttcccacag cgcataagct acgcctaagg cgatcttttt aacggcgaaa gagagaaata
    20401 tatacgacag agaaatcatc accagcatta aaataaagcc gccatttccc tcactgacgc
    20461 tcgcccattt cattgacagc gtaccggtaa tttctgtagc aatagccagg cctaataaaa
    20521 tccaataaat atacattgtc cttctcctgc aagagaatta ttttaatttt cgcttaattc
    20581 agcgaaacca aagtaaagtg gtcaagcccc gcgcataggg caagcttagg cagaagaaag
    20641 gactaaagcg cgttgcgcca gtgctgctca cctacgagca agatagtgga tgaggtacga
    20701 agatgggtaa atgttgaaaa taacgtcctg aacaaattgt ccataatatt acaattatcc
    20761 gcagtgttgc ttctcgtcat cgcggatgat aattgtcctc ggtagttgaa cacgcctgat
    20821 ttgtatcata gcttaagaat taactcaaaa tattttcact tctttacctg agcggtttga
    20881 ttttcgttat gatgacggag cgaaaaagac attattatta gcaaaggaag aaaaaacggg
    20941 gacaagcatg gcaaagccga tcatcacgct caatggccta aaaatcgtca ttatgttggg
    21001 aatgctggtc attattctct gcggtatccg ttttgccgcc gagatcatcg tgccgtttat
    21061 tctcgcatta tttattgctg ttatatataa cttacttcac ataacacgtt gatttaatta
    21121 aataattttc accgcaaaaa gtgccaaaaa tcgcaaaatg actacaccag tgactacacc
    21181 gttcggtgca ctgtatgaaa caacgtggaa caaatagaca caagaaatat acaggcggat
    21241 catctttcca gggggaagcg caccaattca tgaggggcgt taatgtcgat atggggatcc
    21301 ccacctaccg gaattttttt ccggttaaac gttaatcagg ctggtgggct ttacctgttc
    21361 ggtaggtgtt atgattatcg taataccttt cccccaatgg ggtaaaagca tcaaccgtaa
    21421 cgaaagtcgt tatggttgcc actgataccc caaatttggg tatcacgagt aagacccaaa
    21481 atctgggtgt tactcttact acccaaacta cgggtagttt ccgtagtttc atggtcgagt
    21541 tgcagatctg caactccccc attaaccaca ggcgaatttc cgccttcggt cattccttaa
    21601 tcatctgcgt aatcataatg attattcagc aagcgcaatt ttgcgctttg cttaaagttc
    21661 agcgagttac cgcgccgact tcctcaaatt gaggtttccg aaaatatcag cgggttagcg
    21721 attaagctga tcattaatta aactgcgaaa atttcgtagt ttgtgactgt ctttcaggca
    21781 gcttgttaac agtcctcaga agcgagggat gtagccaccc atttttggtc cgtcgggaat
    21841 atcaaccagt taccgccgca accgctccgg cttcttccag tggtacgtta ttttctcctt
    21901 ctcccgatac atctccacgc ggcgacggta ggccagcaac tcaagaactc tggttcgtat
    21961 gttgcgcata tccacgccat taagttctat accatcacgg cgcatcacct cagcaacaac
    22021 acgcgcatag ttttcggctg tcacgctgtc cggctgcgtg gcctgttcgt cagcctgctg
    22081 gctgattccg gcaacgcggc ggattaatcc cgttatttcg gcttctgtca tgctgctgac
    22141 ctcattacac cccgctaaat tcttccagtt tctggcggtg gctgtcgctt atatcaaaag
    22201 caaaatcctc atgctctgcc tggaatgtac caaacgccat cagcgccgcc acgctcgggt
    22261 ctatcttgtt cggtgatttt ttcttgttcg gcttgatatt ggcgttcgcg tcactctgca
    22321 tcacgacgtt actcatcgac caggacaaca ccggatcgcc acgatgcaca atcaccctgc
    22381 ggttaacaaa aacttcaaac gatttcgccg ccggactgaa tctgaggtag gtttgcggga
    22441 acggctccac ctcaaaacct gccccctgta attgcgttct gagatgcgtg gcgttccacg
    22501 tatcaaagcc caccagcctg atattaaatt tctccgcgtc ctgcatgatg tcatctctga
    22561 tccggtcgta atcaatgcag tcgccaggcg ttgtgcgtat ccagcctgct ttagcccact
    22621 gacgatagat ggcgcggttt ttattggcgg ggttctgtag ctggaactcc ggcagataat
    22681 gacgggaaac cagcataatg gttttaccta ccggaaaggc atagcacacg ctggaaatat
    22741 cgcttgtgga tgataaatcc agccccgcgt aacactcctg gccgtgtaaa tcttcctccg
    22801 tgaacgttcc ggcacactca gcccatgcgc cgttacccat ccacggggta gtcccctgac
    22861 accagatatt aaatcgcttt gtcatcatct ccacccactg cgacggaata ccccgcgctt
    22921 tctggatggt tgaggccagt ttttcacgat ccacggaaac atcaatatta gggttcgcct
    22981 ttatccacat cgccggatca tcaacctcgt tttcgtcgtc cagttcgtaa atcagtacaa
    23041 aaattgaatc gttaacctct tcgccgtcca ggatctggca gcaataatca tagtgctgct
    23101 tacaggctga aacgacgttg ctgcctgatg tggtaatggc aaataacagc ccttcgggac
    23161 gtgcgcccat ccccagctca agcgcggaat aaacgccgtt atcggggtgc aagtggtatt
    23221 cgtccacgat ggaaaggctg gggtttgttc cctcgatggt tgccgctttt gctgccagcg
    23281 gctttaacag gctgttgctt ttcgggtgta tcaccttatg cgcctgaata ttcacccgcc
    23341 tgcgtaacgg tcgggataaa aggcacatct gacgcgcatc atcaaagacg atccgcgcct
    23401 ggtcacggct caccgctgcg gtgtagatat cctgctgacc gttctccata atcagaaacc
    23461 agttagccag aatcgcggcg gtcgttgatt tcgcattttt gcgcggcact tcgataaagg
    23521 cgctcgtata tttgcgccgt ccggtggcct taaccttaaa gccgaggatg cacgcaaagg
    23581 cgaactgctg ccacggctcc agctcaatgg gtctgccacg catcggccct tttacgtgcg
    23641 ggcacaccct ggaaaaggca ataaaccgat ccacaacctc acgatcgaac gtgtaaaggg
    23701 ggcttttaag gtccgaaaag taccgtttaa cggcctgttt cagccgttta caggccggaa
    23761 ttttgcccgt ttttacgtct tctgcgtact tattccaggc ggtcaagctc gtcctcttct
    23821 tctgtttccg gtggattttt acggcggctt atcgggtcaa aaccgagcaa ggaggccatt
    23881 tttatcatca ctctttccgc gtcggatttc gcgcttaatg cggggtttct gctctctccg
    23941 ccctgactgt taacaatgct gaacccgcgc gccgcaaggt ctgcgacggc tttccggtat
    24001 atcgagtaat tgacgcaata cagttccaga ttactccagt cggcgggagt caggtcaccc
    24061 cgttccgcaa gctgccgcga tttttcccgc cactgcttca cggcgatatc atccaggtag
    24121 gcgggggctt tcggtggtct tgccatgctt attttttcgc cagattattt ttcaaaaaat
    24181 tcccgtgcat aaaaatttga ggaggcgttc ggtgcccggc ggggtcgggt ttgtcctgaa
    24241 aacgcccccc accccgtcat acagcctcat cagcgattgc ggaaacattc cataacctcg
    24301 cggtcacggt cggttaatcg cttcgctgtg atgcgttctg cgcgtcctga cgctttatct
    24361 ttatgccctg tttcctgtgt cttccatgcg tcacgctgcc ttataagtcc acggataagg
    24421 cggttttgtt cccgctcatt catcagcgtc atacatccag ttattgcggt tagcggcccg
    24481 ttcttcctct tcacgaaatc cacctgcggc acgcttgctt tttgttgccg gatcaagcca
    24541 tttcgttttc tggttatgac acgcctggca caatggttga tggttccatt cgggccagaa
    24601 gagaacatca tcaccgccat taatcgggat aatgtgatcc accaccacgg cgggcgtata
    24661 tatccccttc tcaaggcatc gcacgcataa cgggttttta ctcagataca tggcgcggta
    24721 tttgtcccac tgtctggaat atccacgcgc gcggcggtgt cctcgtctgg catcctctgc
    24781 acgccatgcc gcccgcctgt gctcctcaca cttgccggat ttaacgcgcc tgttacagcc
    24841 tggttctgtg catcgtctta atggttgcca cggcatcagt acacccccac atcacggtaa
    24901 gccgtccaga gtgcgcctat cgtcatgggt acgcgtgttt ttgcgttatc cgcgacaatc
    24961 tggcgatttt catacaggtg agcgataaac atcatgcagc caatctttat ggctggcgtg
    25021 aactccagcc cgtcatcaaa gcgcctgcct atgtgtatct ggcacgcctc aagcgatgcg
    25081 gcaatgtatc cgctgattaa ctggtcttca tcgtcgccat cgatgcggca atggagtttc
    25141 acttcttcca gggtgataag ttcttctgtc atttttccgc gccctcacga caaagaattt
    25201 caaggcgtgt cctggcggca tccggcagcg gctgcccgat gatattcagt acacgccccg
    25261 ccagcggccc cgtactgacc tttatccggc tggtggcgtt gatgtccttc cggtaccgta
    25321 tccagatcct tacggtacca atcgccagct ctgcgcccga tgaaatggcc tccttgctgc
    25381 tgaccatgtt cacgcttgcc cagagtgtgt gaccgtcctc ccacgtttcg agtatttcgc
    25441 cggttatatc cctggtctgt ttcagggtct gaatcgttat cctgtcacgc agtcgcccta
    25501 tgttcattcc ggtttttctc cctcgctgat ttttacttcc tgtttccatg cctggctgaa
    25561 ctcatcacca ccatcacgcg gtgacagacc ttcacgttcg cgggcttcgt tcggacacat
    25621 aacgccggat ttgatgccgc gctcataggt ggcaaagcgt tcgccaggtg tggcccgtaa
    25681 caggtccgcg ctgtcaaact ccacctgata ccggatacca ggtacaggcg aggccaccag
    25741 cagggcgttt tttatctgct gctcaaagtt cgccagccac gggcgcatcg tcatggtgag
    25801 aaatgcgcgg cttgcctcgc tgaaattgct gtaggtgctg ttgctgtatt cctgcaaaaa
    25861 aatcggcgag acgttgaaca ttcgggcgat gtcttcaatg gtgaagcgac gggaggccag
    25921 ccattcagca tcctgattac tcatgcccag ctgctgatag ctcatacccc cttcaaggat
    25981 gggcgttttt ccggcgtttc tggccccttt gtagcgttcc agtgcggcta atgcctgttt
    26041 gcccttcacg ccgtccagcc attcgcctga cgtgataacc cctgccgcca tcatgccatc
    26101 acgcatcacg ctcgcgccgt ggcgttgttg ggccagccca agccccagcg attcgcggca
    26161 gatggttaca ggtgagcgcc ccataaagcc gtcatccgtg gagtaacgaa ggtgaagtac
    26221 ttcccacggt aaatagttgc gggtgtttcc ggtgtaggcg tcagtgatgc agtagcgcca
    26281 gttgtgttcg cctgtctgct ccacgttcac cgactgcggc gggtaaggat gtaaagccgc
    26341 cggaaaacca tcacgccccc actgaatcac cgcataagca ttaccgttta acaggcagtg
    26401 gcggatcatc attcgcttaa actggtaggg ggtttgccat gcgttcgggc gctcgttgag
    26461 gatgtgatcg accggatgag aatcaagcca ctcgcgggcc tccttcccct tctcattgcg
    26521 taccaggtac aggtaacacg gcatggtagc caccgcctca gagatgaccg tcacggcgtt
    26581 catgacggcg ggcagtgatt ccgctgtccc cgatgatacg tactcacccg cccccgtgtt
    26641 cgatgtgcca gccagcgcca taaattcatc cagcgtcatg ctgcgctgct ctttttttct
    26701 tctgaaaggc cacatatcac acccccgcta aatccaccca ccagcggcga ttatccgcac
    26761 gcggcatttt ttcggggtgc tgctcataca gggaacggcg ggccagctcc acgccggaat
    26821 cggggtaagc cggtaccgat gtaacggtaa tttcgtacag ttccgccacc agcacggtgc
    26881 gcacgcatgg atctgttgtg gtatcccata catccttacg ggagcgaaag ccaaagctca
    26941 tccccgagat gtccccacgc ttaaccagtt cgataacgtc gcgccctgta ctggtatccg
    27001 gtggggtcag ttcaaagcgt aaccctgttt cgtcctcttc cagtttcagc gtgccggaac
    27061 gggtgcgccc cagtaacatg ctgtggtcat gctcatacag gcctcgaacg tcattacccg
    27121 ccgcaagcca ctcagtaaac gccccccgct ggaatttttc gtaaaactcc ccccatagca
    27181 gttctgaaag cttatcccag cgaacaacgt agcccgtcag cgtaccggct ccgctggtag
    27241 tgatttccga tgaccggatt tccatactct tcataatttt ttcacccata aaacactaaa
    27301 ggggctttta agccccttct gtatgctgtt aatcgtcgtc ctgtggcagt tccagaatct
    27361 tgatcgcgtt cgaatccacc acgccaccgc ctaaatattt ctgcgtgaaa attttgatga
    27421 agcccggctc tgtgaggttg tccggtctgg tgcgaacacc tgtttcgtga tcaacaatgt
    27481 agtaaccgcg tttgaagtca cccagggcaa taacgttatc cggcataaac tccagatatt
    27541 cgaccggaag gcccagtaac gtatcaggat cacccgcctg taaacggtcg cgccagatgt
    27601 aatcaccgtt cgcgttcttc accttctgaa gttttgccgc cgtcgtggag ttaaccaccc
    27661 agaccgcgtt tttgcggtat tttttacgta atgcaaattt caggtcgatc agcgggtccg
    27721 cagatgtcca cgccagagat tcggaaggtt taattacctg caacgtacca aaatcacgct
    27781 ctttgtcgtt cttctctgca cggggtacgg ataaaaagcc ttttgctttt ttgtcaccgt
    27841 cgcccacaac cagatcgctt tcttcggttt ccgtgaagtt gtcgccaatc tcgcccgtca
    27901 gccatgaaag gatgtccaca tcggaaaaat ccacgatttc ctgcgtggtg cgcgggtacg
    27961 catagaccgg atacagctta atgctcacct cgttaatctg cggggtgctg gtctgttcgc
    28021 gtgccttacc ctcttcaccg tggttaacgg tcgcaccgcc agcggaaaca agctgcttaa
    28081 actcgttgct gctgattttc ttcacggtac agatgcggcg catggtggat tcatccgtca
    28141 gcattcgcat gatttcggtg ttcagttcgg ggataacggt ataaccacca tcagcgggaa
    28201 cgcctgtact taatgcgcgg gtttcacctg tcagaatgta gtgacgtagt tcggctgggt
    28261 cagtggtctg actgctttta cctggcttgc tgcgctcttc gtctgcaatg gcttcaaggc
    28321 gggaaatgtc tttatcgagg gattcagctt tagcgcgtaa ttcgtcaaat tttgcgccct
    28381 cagcatcgtt aagactgcgg ttttcttttt ccgcgttctc cagcatgtcg cgcatctgat
    28441 ttttaatggc ggttttctgc tggcgtaatt cgattattct cggcataaaa aaagtcctgg
    28501 ggttaagtaa ggaactccag gacgcggcaa aaactcaacc gtttttcata aggaaatcag
    28561 caatcgcacc gatcgttttc ccgcctggta atgaatattg gcgagaacat taacaggcgg
    28621 aaaagtggcc ccagcgtcct ggcaccacgg gcgagaataa tcatgattca gttcgggtaa
    28681 aatatgccga tccgctcagt gaacaacgtg gaacaaccac gaacaaataa tttacaaaac
    28741 atgacaaaaa gccggattgc tccggctgtg tgattatctg tcctggtaat ttcgccatat
    28801 ttcattacca gcaccatcca tacccatttc ggcataagtg cgatcgaccg cctttttcag
    28861 gtctccgtag ttatccggcg gctccggtgg cctctgtgcc ttcctggaac attccagccg
    28921 tcgcatcgtg atgtgatgcc gttccttgtc tgtctccacc agttgcatga cttcacccca
    28981 tcgcgccgac gctctccggt aaaagccttt cgcctccagc tcttccgcta tgcggtcatg
    29041 taccatcgtc cccacccctc agaacggaat atcatcaccg taagggtcat cgcctcccgc
    29101 tggtggctga ttaccctgtg tgcctgtggt tttgcgtctg ttcccgccag gacgtgccgc
    29161 acgggcactg attacgctgt ctgcaataac ctgatacccc tgccgtgttt ccccgttctg
    29221 tccggtccac tggcttacct gcatcgtgcc ggatacgctg gcaacatcgc ctttttgatg
    29281 tttagccagg aaatcggcct gtttgccaaa tgcgataacc gataaccata acgtcgcctg
    29341 tccgtcatgt gcctggctgc atggcaacga taccgccata cgcgccagag tcatcggtgt
    29401 gcccttgctg ctctgtttta ccagcgggtc gtccaccagg cgcccgtaag ctgcaatttg
    29461 tgctgtcata ttgccacctc agtgaagcga tccggcgttg ttctttttca tctcttccat
    29521 cattttttca gtgagcatta cgcactgaaa acccatgttt ttgatgtcta cgggtttcat
    29581 gcctttgtca gtggatatca caggcagaat atcctgatac acatcgcggt cagggtaact
    29641 aagcacacaa agcgcccatg atgccacggg tgatgatcct tcgtctgttt caacccacca
    29701 gcctggtgta gctgcgctta tttgctgaat cgtgatccgt ccgttgtgct tgtaaagttt
    29761 ttcaccaaat tttggttcgc tcatgattcc acctctccg
//
LOCUS       DAZGTR010000052        29034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGTR010000052 DAZGTR010000000
VERSION     DAZGTR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.052"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(91..1755)
                     /gene="glnS"
                     /locus_tag="ACHLCY_002492"
     CDS             complement(91..1755)
                     /gene="glnS"
                     /locus_tag="ACHLCY_002492"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP0369734.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1958..3904)
                     /gene="nagE"
                     /locus_tag="ACHLCY_002493"
     CDS             complement(1958..3904)
                     /gene="nagE"
                     /locus_tag="ACHLCY_002493"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP0369735.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4237..5037
                     /gene="nagB"
                     /locus_tag="ACHLCY_002494"
     CDS             4237..5037
                     /gene="nagB"
                     /locus_tag="ACHLCY_002494"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0369736.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5097..6245
                     /gene="nagA"
                     /locus_tag="ACHLCY_002495"
     CDS             5097..6245
                     /gene="nagA"
                     /locus_tag="ACHLCY_002495"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0369737.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6254..7474
                     /gene="nagC"
                     /locus_tag="ACHLCY_002496"
                     /gene_synonym="nagR"
     CDS             6254..7474
                     /gene="nagC"
                     /locus_tag="ACHLCY_002496"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP0369738.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7522..8274
                     /gene="nagD"
                     /locus_tag="ACHLCY_002497"
     CDS             7522..8274
                     /gene="nagD"
                     /locus_tag="ACHLCY_002497"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP0369739.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8531..10195
                     /gene="asnB"
                     /locus_tag="ACHLCY_002498"
     CDS             8531..10195
                     /gene="asnB"
                     /locus_tag="ACHLCY_002498"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP0369740.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALEN
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10576..10652
                     /locus_tag="ACHLCY_002499"
     tRNA            10576..10652
                     /locus_tag="ACHLCY_002499"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10610..10612,aa:Met,seq:cat)
     gene            10661..10745
                     /locus_tag="ACHLCY_002500"
     tRNA            10661..10745
                     /locus_tag="ACHLCY_002500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10695..10697,aa:Leu,seq:tag)
     gene            10769..10843
                     /locus_tag="ACHLCY_002501"
     tRNA            10769..10843
                     /locus_tag="ACHLCY_002501"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10801..10803,aa:Gln,seq:ttg)
     gene            10878..10952
                     /locus_tag="ACHLCY_002502"
     tRNA            10878..10952
                     /locus_tag="ACHLCY_002502"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10910..10912,aa:Gln,seq:ttg)
     gene            10968..11044
                     /locus_tag="ACHLCY_002503"
     tRNA            10968..11044
                     /locus_tag="ACHLCY_002503"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11002..11004,aa:Met,seq:cat)
     gene            11093..11167
                     /locus_tag="ACHLCY_002504"
     tRNA            11093..11167
                     /locus_tag="ACHLCY_002504"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11125..11127,aa:Gln,seq:ctg)
     gene            11205..11279
                     /locus_tag="ACHLCY_002505"
     tRNA            11205..11279
                     /locus_tag="ACHLCY_002505"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11237..11239,aa:Gln,seq:ctg)
     gene            complement(11433..12608)
                     /gene="ubiF"
                     /locus_tag="ACHLCY_002506"
     CDS             complement(11433..12608)
                     /gene="ubiF"
                     /locus_tag="ACHLCY_002506"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP0369741.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12754..14178
                     /gene="miaB"
                     /locus_tag="ACHLCY_002507"
     CDS             12754..14178
                     /gene="miaB"
                     /locus_tag="ACHLCY_002507"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP0369742.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14331..15371
                     /locus_tag="ACHLCY_002508"
     CDS             14331..15371
                     /locus_tag="ACHLCY_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP0369743.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PVYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15368..15835
                     /gene="ybeY"
                     /locus_tag="ACHLCY_002509"
     CDS             15368..15835
                     /gene="ybeY"
                     /locus_tag="ACHLCY_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP0369744.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15925..16803
                     /gene="corC"
                     /locus_tag="ACHLCY_002510"
     CDS             15925..16803
                     /gene="corC"
                     /locus_tag="ACHLCY_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP0369745.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16828..18366
                     /gene="lnt"
                     /locus_tag="ACHLCY_002511"
     CDS             16828..18366
                     /gene="lnt"
                     /locus_tag="ACHLCY_002511"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP0369746.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGTGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18444..20321)
                     /locus_tag="ACHLCY_002512"
     CDS             complement(18444..20321)
                     /locus_tag="ACHLCY_002512"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP0369747.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASEAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20776..21684
                     /gene="gltI"
                     /locus_tag="ACHLCY_002513"
     CDS             20776..21684
                     /gene="gltI"
                     /locus_tag="ACHLCY_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP0369748.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKSGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21854..22594
                     /gene="gltJ"
                     /locus_tag="ACHLCY_002514"
     CDS             21854..22594
                     /gene="gltJ"
                     /locus_tag="ACHLCY_002514"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP0369749.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22594..23268
                     /gene="gltK"
                     /locus_tag="ACHLCY_002515"
     CDS             22594..23268
                     /gene="gltK"
                     /locus_tag="ACHLCY_002515"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP0369750.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23268..23993
                     /gene="gltL"
                     /locus_tag="ACHLCY_002516"
     CDS             23268..23993
                     /gene="gltL"
                     /locus_tag="ACHLCY_002516"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP0369751.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            24111..25046
                     /gene="rihA"
                     /locus_tag="ACHLCY_002517"
     CDS             24111..25046
                     /gene="rihA"
                     /locus_tag="ACHLCY_002517"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP0369752.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25130..26800
                     /gene="hscC"
                     /locus_tag="ACHLCY_002518"
     CDS             25130..26800
                     /gene="hscC"
                     /locus_tag="ACHLCY_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP0369753.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26860..28311)
                     /gene="djlC"
                     /locus_tag="ACHLCY_002519"
     CDS             complement(26860..28311)
                     /gene="djlC"
                     /locus_tag="ACHLCY_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFP0369754.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28308..29015)
                     /locus_tag="ACHLCY_002520"
     CDS             complement(28308..29015)
                     /locus_tag="ACHLCY_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0369755.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSCVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         7127 a         7547 c         7341 g         7019 t
ORIGIN      
        1 aaaacgccgc agagtgcggc gtttgggatt gtttgctatt tcttgccgga tgcgacgtaa
       61 atgcctcacc cggcctggta gaacttaaaa ttactcgcct actttcgccc aggtatcacg
      121 cagcccaacg gtgcggttaa ataccggttt ttccgctgtg gaatggcggc tgtcgaggca
      181 gaagtaacct tcacgctcaa actggaatgc tttacccgca accgcatctt tcagcgacgg
      241 ttcagcaaag ccctgtttga tcaccagcga ttccgggtta atcaccgaca ggaaatcatc
      301 cgcagcacca gggttcggca cgctgaacag acggtcgtac agacggattt caaccggcag
      361 cgcatgtgcc gcgctcaccc agtgaataac acctttgact ttacgaccat ctgccggatc
      421 tttgcttaag gtgtcggcgt cataagtaca gaagatggtg gtgatattgc cttcggcatc
      481 tttctccacg cgttcagcct taatgacgta agcattacgc agacgcactt ctttacccag
      541 caccagacgt ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat
      601 ctcaccgcta aacggcacct gacgactccc catttccggt ttgttcggat ggttcggcat
      661 ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc agtttcaccg gatcgataac
      721 cgccattgcg cgcggcgcat tttcgttgag atcttcacgg atgcaggatt ccagcgatgc
      781 catctcaatg gtgttgtcct gcttggtcac gccgatgcgt ttgcagaact cacgaataga
      841 agcagcagtg taaccacgac gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca
      901 gccttcaacg tgcttgtcgg tcaccagcag gttcagctta cgcttggaca tcacagtgta
      961 ttccagattc aggcgcgaga attcgtactg gcgcgggtga acaggaatgg tgatgttgtc
     1021 cagcacccag tcgtacaggc gacggttgtc ctggaactca agcgtacaca gagagtgcgt
     1081 aataccttcc agggcatcgc tgatgcagtg ggtgaagtcg tacatcgggt agatgcacca
     1141 cttgttgcca gtctggtggt gttcagcaaa tttaatacgg tacagcaccg gatcgcgcat
     1201 cacgataaac ggtgaagcca tgtcgatttt cgcacgcagg caggctttac cttcttcaaa
     1261 accaccggca cgcatttttt cgaacagcgc caggttctct tcaacgctgc ggtcgcggta
     1321 cgggctgttt ttacccggtt gcgtcagggt gccgcggtat tcgcggatct gttccggcgt
     1381 cagttcatca acgtacgcca ggcctttatt gatcagttcg atcgcatagg cgtggagctg
     1441 atcaaaataa tcggaggagt aacggacgtt accagaccag tgaaaaccta accactctac
     1501 gtcgtttttg atcgactcaa catactcgat atcttctttt accgggttag tgtcgtcgaa
     1561 acgcaggttg cactggcctt tatagtcctg ggcgatcccg aagttcaggc agatagattt
     1621 cgcatggcca atatgcagat agccattcgg ctccggcggg aaacgggtgt gtactgtggt
     1681 gtgcttacca ctggccagat cttcatcgat gatctgacgg ataaagttag tcgggcgggc
     1741 ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct
     1801 tataagcggg acaggctgac aactgttagt tttttaaaat tgataatcta acaaggatta
     1861 tggggatgat tgatgaaaaa aaaagcggca gagaagatct ccaccgcttg tcattgttgg
     1921 atgcgacgct caagcgtcgc atcaggcata aagcagatta ctttttgatt tcatacagcg
     1981 gtgtttgacc cgccacaaca tggccctgag ctttaatgat caagccactg aaatcgtcga
     2041 tattgctgca aaccaccggg ctaatcatcg agcgggcgtt agcgttcagg taatccagat
     2101 ccatttccag aatcggttgc cctgcgctta cctgcgcacc ctcttccacc agacgtttaa
     2161 agcctttacc ttccagcgct acggtgtcga tacccatatg gacgacgatc tccgcgcctt
     2221 tttcggtttc caggcagaac gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg
     2281 ctggtgatac gacgatttta tctgtcggtt tcaccgccac accgtcaccc accgctttgc
     2341 tggcgaatgc ttcgtcagga acctgatcca gtgccacgac atcaccggta atcggcgata
     2401 ccagctccgc gatagatacc gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag
     2461 ttgccggagt tgcttcagct gacgcagcgg ctaccggacc acgggcaacg actttcttca
     2521 tcgcatcgcc gatggattct gctttcgcgc caacaatcac ctgaatagtc tgtttgttca
     2581 gtttcactac accagaagca cccagacgtt tacacatcgt atcgttgacg cgggcagagt
     2641 cagccacggt caggcgcaga cgggtgatac aggcgtcaat tgctttcagg ttgtcagtgc
     2701 cgccaaccgc agcaatatag ttggtcgcca gttgattgag gccttcttca gtgttgctgt
     2761 tggcttcttc agtaacgatc tcgtcttctt tatcttcacg acccggcgtt ttcaggttga
     2821 acatgcggat aaccaaactg aacaccacga agtagatagc gaagaagaca acacccataa
     2881 ccagcagcat ccagacgttc tggctggcgg ccggcaggtt atacatcaac gcgtagtcga
     2941 tagcccccgc tgagaaagag aagccagcat ggatacccag cagtgttgcc acaaacaggc
     3001 tgataccggt cagcagtgcg tgcaggaggt acagcagcgg tgcaaggaac atgaacagga
     3061 attccagcgg ctcagtcaca ccggtcagga acgcagtaac agcaacagaa agcagcatcc
     3121 cgccaaccat cggacgacgc tctttcggtg ctgcgaagta catcgccagc gccgcacccg
     3181 gcagaccgaa catcataatc gggaagaagc cggacatgaa catccccgcg gtgccgtcac
     3241 cggcatagaa gcggttgata tcaccgtgga aaaccgtacc cgccgcgttg gtgaattcac
     3301 caatctggaa ccaggcgatg gtgttcagca cctgatgcag accggttggg atcagcagac
     3361 ggttgatgaa accaaagata ccggaaccca gcgcgcccgc agaaacgatc cactcgcctc
     3421 ctgcatggat agcgtgctgt accggcggcc agacgtaacc aaaaatggcc gccagcacca
     3481 ggcagaagaa gccggtggca atcggcacaa agcgtttgcc gccgaagaaa ctcaggaagt
     3541 ccggcagttt aatatcggac caacggttat aggctgcgcc accaaccaga ccggtaatga
     3601 tacccgccag tacacccatg ttaatttctg ggttgatggt caccatcgct ttggttaaca
     3661 caaagtaacc taccgcaccc gccagcgccg ccgcacctgc gctgtctttc gaccagctgg
     3721 atgccacacc gatggcgaag attaatgcga ggttatcaaa aatcgcaccg cccgcctggg
     3781 caataaacgc aacgttaagt aaatctggct gaccgaatcg cagcaacagt gccgccaccg
     3841 gcagcaccgc gatagggagc tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat
     3901 tcatcttatt ccccctacga gaaccctatt tggctcgttt aaagccgtat ttttattttg
     3961 ctgcaaattg tactgccgat gttctgtaat cagattgtta gatcatctgc tacagagtgt
     4021 gtgaaaattt aattcgtatc gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa
     4081 tatcgttatc atcactccct tttactggct aaagcagaaa acttatttta tcattcaaaa
     4141 aatcaggtcg gattgacgcc tgtctgcgca aatccaggtt acgcttaaag atgcctaatc
     4201 cgccaacggc ttacatttta cttattgagg tgaataatga gactgatccc cctgactacc
     4261 gctgaacagg tcggcaaatg ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa
     4321 ccgactgccg atcgtccgtt tgtactgggc ctgccgactg gcggcacgcc gatgaccacc
     4381 tataaagcgt tagtcgaaat gcataaagca ggccaggtca gctttaagca cgttgtcacc
     4441 ttcaacatgg acgaatatgt cggtctgccg aaagagcatc cggaaagcta ctacagcttt
     4501 atgcaccgta atttcttcga tcacgttgat attccagcag aaaacatcaa ccttctcaac
     4561 ggcaatgccc cggatatcga cgccgagtgc cgccagtatg aagaaaaaat ccgttcttac
     4621 ggaaaaattc atctgtttat gggcggtgta ggtaacgacg gtcatattgc atttaacgaa
     4681 ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc
     4741 gcgaactctc gtttctttga taacgatgtc aatcaggtgc caaaatatgc cctgactgtc
     4801 ggtgttggta cattgctgga tgccgaagaa gtgatgattc tggtgctggg tagccagaaa
     4861 gcactggcac tgcaggctgc agttgaaggt tgcgtgaacc acatgtggac catcagctgt
     4921 ctgcaactgc atccaaaagc gatcatggtg tgcgatgaac cttccaccat ggagctgaaa
     4981 gttaagactt taagatattt caatgaatta gaagcagaaa atatcaaagg tctgtaattg
     5041 ttatccctgc cctggctcct tgctcagggc aatatttttt aaaatcgggg gtcagaatgt
     5101 atgcattaac ccagggccgg atctttaccg gccacgaatt tcttgatgac cacgcggttg
     5161 ttatcgctga tggcctgatt aaaagcgtct gtccggtagc ggaactgccg ccagagatcg
     5221 aacaacgttc actgaacggg gccattctct cccccggttt tatcgatgtg cagttaaacg
     5281 gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa
     5341 tcatgcagaa agccaatgag aaatcaggct gtactaacta tctgccgacg cttatcacca
     5401 ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg cgagtacctg gcaaaacatc
     5461 cgaatcaggc gttaggtctg catctggaag gtccgtggct gaatctggta aaaaaaggca
     5521 cccataatcc gaattttgtg cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa
     5581 acgccgacgt cattaccaaa gtgaccctgg caccggaaat ggttcctgcg gaagtcatca
     5641 gcaaactggc aaatgccggg attgtggttt ctgccggtca ctccaacgcg acgttgaaag
     5701 aagcaaaagc cggtttccgc gcggggatta cctttgccac ccatctgtac aacgcgatgc
     5761 cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt
     5821 attgcggtat tattgctgat ggcctgcatg ttgattacgc caacattcgt aacgctaaac
     5881 gtctgaaagg cgacaaactg tgtctggtta ccgacgccac cgcgccagca ggtgccaaca
     5941 ttgaacagtt catttttgcg ggtaaaacaa tatactaccg taacggactt tgtgtggatg
     6001 agaacggtac gttaagcggt tcatccttaa ccatgattga aggcgtgcgt aatctggtcg
     6061 aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg
     6121 cgattggcgt tgagaaacgt ctcggcacac tcgccgcagg taaagtagcc aacctgaccg
     6181 cattcacacc tgattttaaa atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc
     6241 aataagagaa agtatgacac caggcggaca agctcagata ggtaatgttg atctcgtaaa
     6301 acagcttaac agcgcggcgg tttatcgcct gattgaccag tacgggccaa tctcgcggat
     6361 tcagattgcc gagcaaagcc agcttgcccc cgccagcgta accaaaatta cgcgtcagct
     6421 tatcgaacgc gggctgatca aagaagttga tcagcaggcc tccaccgggg gccgccgcgc
     6481 tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca
     6541 cgacgccacc atcaccctgt ttgatctcag cagcaaagtg ctggcagaag aacattaccc
     6601 gctgccggaa cgtacccagc aaacgctgga acatgccctg ttgaatgcca ttgctcagtt
     6661 tattgatagc taccagcgca aactgcgcga gctgatcgcg atttcggtga tcctgccagg
     6721 gcttgttgac ccggacagcg gcaaaattca ttacatgccg catattcagg tagaaaactg
     6781 ggggctggta gaagcactgg aagaacgttt taaagtgacc tgtttcgttg gtcacgatat
     6841 ccgtagtctg gcgctggcgg agcactactt cggtgcaagt caggattgcg aagactccat
     6901 tctggtgcgt gtccatcgcg gaaccggggc cgggattatc tctaacgggc gcatttttat
     6961 tggccgcaac ggcaacgtcg gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg
     7021 ctgccactgc ggcaactttg gctgcctgga aactatcgct gccaacgccg ctattgaaca
     7081 acgggtgttg aatctgttaa agcagggcta ccagagccgc gtgccgctgg acgactgcac
     7141 catcaaaact atctgcaaag ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga
     7201 gtatgtcggt cgtcatctgg gcaaaaccat cgccattgct atcaacttat ttaatccgca
     7261 aaaaattgtt attgccggtg aaatcaccga agccgataaa gtgctgctcc ctgctattga
     7321 aagctgcatt aatacccagg cgctgaaggc gtttcgcact aatctgccgg tggtacgttc
     7381 tgagctggat caccgctcgg caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa
     7441 cggtattttg ctccagcatt tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt
     7501 tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg
     7561 ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat ttttgcacgg gattatggat
     7621 aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc agaccgggca agatctggcg
     7681 aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg
     7741 gcgactgccg atttcctgcg tcgccaggaa ggcaagaaag cgtatgtggt gggcgaaggc
     7801 gcactgattc atgaactgta caaagccggt ttcactatta ccgatgtgaa ccctgatttt
     7861 gtgattgttg gcgaaacgcg ttcctacaac tgggacatga tgcataaagc ggcctatttc
     7921 gtcgctaacg gtgcacgctt tatcgccacc aacccggaca cccacgggcg cggtttttat
     7981 cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct ccgggcgcaa accgttctat
     8041 gttggtaagc ccagcccgtg gatcatccgc gcggcattaa acaaaatgca ggcgcattcg
     8101 gaagaaacgg tgattgtcgg cgataacttg cgcaccgaca ttctggcggg cttccaggcg
     8161 ggtctggaga cgattctggt gctttctggt gtttcatcgc tcgacgatat cgacagtatg
     8221 ccgttccgcc caagctggat ttacccgtcg gtcgctgaaa tcgacgttat ctgaatagca
     8281 accacgtctc agggcgtggt ttcttatttt tacccatcat taaaatcatc ccattcaata
     8341 aaactcgcca aaaattatcg atccatcaat aaatacatca ccacaccctc ccctttttca
     8401 caaatcagca atcaccatta cgttttttat ttttgcactg ccaaatcgct tgcgcacggt
     8461 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
     8521 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
     8581 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
     8641 tatttatgcc agcgataacg ccattctcgc ccacgaacgt ctgtcaattg ttgacgttaa
     8701 cgcgggggcg caacctctct acaaccaaca aaaaactcat gtgctggcgg taaacggtga
     8761 aatctacaac caccaggcac tgcgcgccga atatggcgat cgttatcagt tccagaccgg
     8821 atctgactgc gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
     8881 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcat atctgattgg
     8941 tcgcgaccat ctggggatca tcccactgta tatgggctat gacgagcacg gtcagctgta
     9001 tgtggcctca gaaatgaaag ccctggtgcc agtttgccgc acgattaaag agttcccggc
     9061 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
     9121 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
     9181 aaattccgtt aaaagccatc tgatgtctga cgttccttac ggtgtgctgc tctcgggtgg
     9241 tctggattcc tcaattattt ccgcgatcac caagaaatac gccgcccgtc gcgtggaaga
     9301 tcaggaacgc tctgaagcct ggtggccgca attacactcc tttgctgtag gtctgccggg
     9361 ttcaccggat ctgaaagcag cccaggaagt ggcgaaccat ctgggcacgg tgcatcacga
     9421 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
     9481 aacctatgat gtgacaacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
     9541 caaggcaatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
     9601 ttatctttac ttccacaaag caccgaatgc caaagaactg catgaagaga cggtgcgtaa
     9661 actgctggcc ctgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
     9721 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
     9781 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
     9841 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
     9901 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
     9961 actggaaact gcccgcttcc gcttcccgta caacacgcca acctcaaaag aagcgtatct
    10021 gtaccgggag atctttgaag aactgttccc gcttccgagc gccgctgagt gcgtgcctgg
    10081 cggtccttcc gtcgcgtgtt cttccgctaa agcgattgag tgggatgagg cgttcaagaa
    10141 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    10201 gcgaaacagc cccggtgaaa tccggggctg tttcagttat tgaggtgctt gttaattctg
    10261 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    10321 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    10381 acgacaaaag aatggctgta gggtggggaa aaaacacaaa gtaaacaata attgacgaat
    10441 atagcgccac gctgttcgca acctaaccaa acagtcactt tcgagcaatt ttccttgaaa
    10501 aagaggttga cgctgcaagg ctctatacgc ataatgcgcc ccgcaacgcc gataaggtat
    10561 cgcgaaaaaa aagatggcta cgtagctcag ttggttagag cacatcactc ataatgatgg
    10621 ggtcacaggt tcgaatcccg tcgtagccac catctttttt gcgggagtgg cgaaattggt
    10681 agacgcacca gatttaggtt ctggcgccgc aaggtgtgcg agttcaagtc tcgcctcccg
    10741 caccattcac cagaaagcgt tgtacggatg gggtatcgcc aagcggtaag gcaccggttt
    10801 ttgataccgg cattccctgg ttcgaatcca ggtaccccag ccatcttctt cgagtaagcg
    10861 gttcaccgcc cggttattgg ggtatcgcca agcggtaagg caccggtttt tgataccggc
    10921 attccctggt tcgaatccag gtaccccagc catcgaagaa acaatctggc tacgtagctc
    10981 agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc cgtcgtagcc
    11041 accaaattct gaatgtgtcg aatacgttcg gcaaattcaa aaaccaattt gttggggtat
    11101 cgccaagcgg taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc
    11161 ccagccaatt tattcaagac gcttacatcg taagtgcgcc cagttggggt atcgccaagc
    11221 ggtaaggcac cggattctga ttccggcatt ccgaggttcg aatcctcgta ccccagccac
    11281 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gttcattcaa tgtcgaatgc
    11341 gatgttgaca cgtcttatcc ttcaatgtcg gatgcgacgc tgccgcgtct tatccgacct
    11401 acggttggca cgcatccggc aatgttgtaa ggctacaacc ctaacgcata tttcagcgcc
    11461 tgacgtttca acacgccagc acgctctgcc gccattaacc cgagattacg cacaaaacgc
    11521 agcggcggca gattattact gaatccggca taaaacagat ccataccact ttgcataatg
    11581 aagttatccg ccatgcgccg catctggtaa cgcttgagga caggataact ggcccacgct
    11641 tcaccgtagc tgcgggcatt caccagaaca tcaatcaggg catcgacatc acgataacca
    11701 agattcaccc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagcgca
    11761 agccccggct gcacatactg caacgcatgt cggcgcgtca gcggaaacgc accagcggca
    11821 agcggtgtaa cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctggagttgc
    11881 gccatattca tattctgcaa ctggcgaata cgtaccggag agtcatacca caccagcgat
    11941 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
    12001 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatca acatacacga ctgcgcatac
    12061 tgccaggcat gaacgccaat tcccgccatc tgccgcacct gcgaatttgc gccgtcagca
    12121 ccaatcacca gcttcgcgcg aatcacttca ccgcccttca gttccagctc ctgaagatca
    12181 ttatctcgat gtaatgcaat cagcgagcct ggcacacgta acgttacttt cggatgcgct
    12241 tccagcgcct gccacaatgc ctgttgcagg acggtgtttt ccaccatata gccaagcagc
    12301 ggcagcttaa gttcagcggc gtcaaacatc acatgcgccg tttcccactc ccacgtttcc
    12361 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
    12421 agcaatgata ccgatgcggc gctgatcgcc gagatccgca cgtccggttg gctatcagcg
    12481 acaaacggtg caggttctgc gtgctcgatc accgttaccg aaaatccgtg ctgtgccagc
    12541 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
    12601 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
    12661 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
    12721 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
    12781 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
    12841 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctgctgct gaacacctgc
    12901 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
    12961 aaagagaaga atccagacct gattatcggc gtcggtggct gcgtggcatc gcaagaaggc
    13021 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
    13081 cgtctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
    13141 ttcccggaaa tcgagaagtt tgaccgtctg ccggaaccgc gcgccgaagg gccgaccgcg
    13201 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
    13261 cgtggtgaag aggtaagccg tccgtccgac gatattctgt ttgagatcgc ccagcttgcg
    13321 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
    13381 aactacgacg gcaccaccgg atcgtttgcc gatctgttgc gtctggttgc tgcgatcgac
    13441 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
    13501 gaagtgtatc gcgacacgcc ggagctggtg agcttcctgc atctgccggt acagagcggt
    13561 tccgatcgca ttctgaacct gatgggccgt actcatacgg cactggagta caaagcgatc
    13621 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt cattgttggc
    13681 ttccctggcg aaaccaccga agacttcgag aaaacgatga agctgattgc cgacgtcaat
    13741 ttcgacatga gctacagctt tatcttctct gcacgtccgg gtacaccagc cgccgatatg
    13801 gttgatgatg ttccggaaga agagaagaag caacgtctgt atattctgca agaacgtatc
    13861 aatcagcaag cgatggcatg gagccgccgg atgctcggca ccacccagcg tattctggta
    13921 gaagggactt cgcgtaagag catcatggag ctttccgggc gtaccgaaaa taaccgcgtg
    13981 gtcaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
    14041 gtctacccga actctctgcg cggtaaagtg gtgcgtactg aagatgaaat gggtctgcgc
    14101 gtggcagaaa caccggaatc ggtgattgcc cgtacccgca aagaaaacga ccttggcgtg
    14161 ggttattatc agccgtaatt ctcaggcccg ccgttccggt gggccttgtt ttttctctct
    14221 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
    14281 taggccggtg aatgttcagc ttccggcgac atacagataa gaggaacggt ttgaacatag
    14341 acactcgcga aatcaccctg gagccagcag acaacgcgcg tctgttgagc ctgtgcggcc
    14401 cgtttgatga caacatcaag cagctcgaac gccgtctcgg catcgagatc aatcgccgcg
    14461 acaaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
    14521 gtagcctgta tgtcgataca gccccgatgc gcggtcagat tcaggatatc gaaccggaac
    14581 agatccacct tgcgattaaa gaagcgcgag tactggagca aagtgcggag agcgtgccgg
    14641 tgtacggcaa agcggtcaat atcaaaacca aacgcggcgt aattaagccg cgcacgccaa
    14701 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
    14761 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
    14821 agattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
    14881 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccgctgtac gacgcgctgt
    14941 ttgaaatgct gggctttgag aaagttgaga aactgattga gcgcaacgtt attgaagtcg
    15001 cgccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt ctcgatgaga
    15061 gccagaacac caccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
    15121 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatact aaatcaggct
    15181 tacgtcacgc catcgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
    15241 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcctat gaagcctggg
    15301 aagaagccga acaaaaacga aaagcggcgc tggcggcaga acgcaagcgc gaagaacagg
    15361 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
    15421 accggaagag agccagtttc aaacatggct gaatgcggtg atcccgcagt ttcaggaaga
    15481 atcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
    15541 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtgc cgcctggcat
    15601 ggaaatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
    15661 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
    15721 tttgttaggt tacgatcaca tcgaagatga cgaagcagaa gaaatggaag ccctcgaaac
    15781 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
    15841 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
    15901 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
    15961 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
    16021 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
    16081 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
    16141 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
    16201 gatgtcatca tcgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
    16261 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
    16321 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
    16381 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgttat tgacgaattc
    16441 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
    16501 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacctgg
    16561 accgtgcgcg cactggcttc cattgaagac ttcaacgaag cgttcggcac ccactttagc
    16621 gatgaagaag tcgacactat cggtggtctg gtgatgcagg cgtttgggca tcttccggcg
    16681 cgtggcgaaa ctatcgacat cgacggttac cagttcaaag tggcgatggc cgatagtcgg
    16741 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccaaa gctggatgaa
    16801 taaaaccgaa actggataga taactacatg gcttttgcct cattaattga acgccagcgc
    16861 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
    16921 gatgtctggc ctgcggcgat tgtttcgctg atggggcttc aggcgctgac ctttaaccgc
    16981 cgcccgctcc agtctgccgc tattggcttc tgctggggat ttggcctctt tggcaccggt
    17041 attaactggg tctatgtcag catcgcgacc tttggcggga tgcctggccc ggttaacatc
    17101 ttcctggtgg tgctgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
    17161 ctgtcgcgcc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccgc ccctgctctc
    17221 tggcaagtga ccgagtttct gcgcggttgg gtgctgactg gcttcccgtg gttacagttc
    17281 ggctatagcc agattgatgg cccgttaaaa gggctggcac caataatggg cgtggaagcc
    17341 attaacttcc tgctgatgat ggttagcggc ctgctggcac tggcgttagt caaacgcaac
    17401 tggcgtccgc tggtggtggc cgtcgtgctg tttgcccttc ccttcccgct gcgttacatc
    17461 caatggttta ccccgcaacc ggagaaaacc attcaggttt cgatggttca gggcgatatt
    17521 ccgcaatcac tgaaatggga cggagaccag cttcttaata cgctgaagat ttactacaac
    17581 gcaacggcac cgctgctggg caaatcatcg ttgattatct ggccggagtc ggcgataacc
    17641 gatctggaaa ttaatcagca accgttcctc aaagcactgg acggtgagtt gcgtgataaa
    17701 ggtagctcgc tggtaaccgg gattgtcgac gcacgtctca ataagcagaa ccgctacgat
    17761 acctacaaca ccatcatcac gctgggtaaa ggtgcaccgt acagctacga atcagccgat
    17821 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccgctgga gtcgattctg
    17881 cgtccgttag caccgttctt tgatctgccg atgtcgtcgt tcagccgtgg gccatatatc
    17941 cagccgccgc tgtcggtaaa tggtattgag cttactgcgg ccatttgcta cgagatcatt
    18001 cttggcgagc aagtgcgcga taacttccgc ccggataccg actatctgct gactatctcc
    18061 aacgatgcgt ggtttggtaa gtctattggt ccatggcagc acttccagat ggcgcgaatg
    18121 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
    18181 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcacccgcga ggtgttgacc
    18241 actaatgtga caccgaccac cggactcaca ccgtatgcac gtaccggcaa ctggccgctg
    18301 tgggtgctga cggcgctgtt tggttttgcc gctgtgctga tgagtctgcg agcacgtaaa
    18361 cgttgatgtg cctgatgtat cctgcatgag caaaacgtac tttctcattg agctttgggg
    18421 catggttatc atgccccttt ttcttacggt tcagggatac aagccacatt gccatattgg
    18481 ggatgacgaa taattgcctg cggatcaagc gagcgcgtcc tgtccatttg gatcaacccg
    18541 ttcttcgcca tgacaaatat ttttctacca tccgctgaaa aacgaaggtg attaacgtag
    18601 ctggcaggat aaacgccaat aatctgcccg gcattaatat tgagcaggat gacataacct
    18661 tgcaggccat atgacaccaa cgctaaatat tgaccgtccg ggctaatacc ctcaacggaa
    18721 taaacgctcc tgctacacca ttgcgtcata atgctggctt gttgatcccc tacgcttata
    18781 cgtgaaatcc cctgactatc ggcaaaaata acctcgccgt tttcaggtcg aaaacgcatc
    18841 gccgatatcg ttccacgcgc atcacctgcg ggaaagtctt ctttctgatg cgtgatggta
    18901 ttgatccgca cgatgttacc tctgcctgct cccggaacca gtaatgttct ttcatccggg
    18961 gaaagtgcca taacccaggc gcctggttta ccgctcgtcc ccgtcccgtc gtatttctcc
    19021 agcgggatat ccgccacatg ctgataagtc accagatcaa tcgctgacac actattactg
    19081 gtgcgattca tcacccaggc ttttgtgcca tcacgcgaga ggataaaact gtcaggatgt
    19141 gcaccggtgg taatcgactg aataatctcc cctgtcgcca cgttaatgac cgataacgca
    19201 ttcgcttcaa atgacgtcgc ataaatcaac ttttcatccg ggcttaatgc cagcgatctg
    19261 acgccctgat ctttacagcc accacaatgt ttgactgact tttctttcgc tatgggcgct
    19321 gtaaaggtat gcaggatttt cttttgtccg aggtcgaaaa caacaatgct gttggtgttt
    19381 tcaattgcgg catacaacgt attaccatcc cgactgatcg ccatatcatg gatatcggca
    19441 atctcagcga gtaaatcacc actggcctgt tcacgactta ccggagcgga taatgtttcc
    19501 ctgagggcgt tgcgttcctc cgctaactgt tgtttttttt gtcgtcgctc ttgttcaatc
    19561 tcctgcggat aaaacgcttc acgcaggctt tgcctgacct gtaaaactga ctcatccatc
    19621 tgctgctgcg caagccagat agccccggtc agaagaagac tgcccgcaac aataatcccg
    19681 ccttccgtaa cgagtcggtt ttgcccagat aaacgcgcgc tcagccaacc caacgcgcca
    19741 cccgcaatca ggccgccaat gtggcaagcg ttatctattc ctgtttgccg ggcaccgtac
    19801 aacagtgtca gcgccaccat acccaacaga ggaaatactc gccgctggtt tttatggtat
    19861 gtgcccgtac ctgaagcaaa ctgtgttgct atcgctgcac cagctattcc cagaatcgcc
    19921 tcggatgccc caacgctgat gactatctgc tcactttcgc gtaacgcata acaggcgctg
    19981 atgagcccac caaagacgcc ggagaataac caggtaatca ggagtcgcca tttccccaga
    20041 atctgttccg ccacgcctcc cacgaccagg aatgcaaaca tattcatcag cagatgagaa
    20101 acactactgt gcagaaagat actgctcaat aagcgccagg gttcacccga caaagtaaga
    20161 ggcgcaacgt ttgcaccgag atggataagt gcggattgat cgacctcttc ataactcatc
    20221 cattgtgcga gataacaaaa tatcccaata cagagtgcaa aaaggataag ggttattgcg
    20281 ggtaattgaa catttaacgg cttaaccgaa gatgcagaca taaggtgttt cttccttgaa
    20341 aagacaggta aaaagcattt ttcccgtgtg cagttatcat cccggcaaac gtcccatgcc
    20401 gaaaagataa ttctgtccga aatatgactt tttcagccta aaattacaat cgtaattaat
    20461 tcgcccctaa gtattttttg gcatgtaagt tgctttattt actgtgtaag cgatgaaatc
    20521 agccgcgacg caaccaggtt gcaccaccac aaatcagcgg tagcaccaaa aaggtgcggt
    20581 ttaagaattt tgcttcggaa tggtgcggcg catctcgcaa aaacaaacaa taaaacagca
    20641 atatctttac atttagccaa actaaatgtt acctaacagg cacaacactg cacaataaag
    20701 ttgaagacga taacaacaca aacactcaca acgggtatcc atgcgttctt aacgcagaag
    20761 ataaaggagt tggatatgca attacgtaaa cctgccacag caatcctcgc cctggcgctt
    20821 tccgcaggac tggcacaggc agatgacgcc gccccggtag cgggcagtac gctggacaaa
    20881 atcgccaaaa acggcgtgat tgtcgtcggt caccgtgaat cttcagtacc tttctcttat
    20941 tacgacaatc agcaaaaagt ggtgggttac tcgcaggatt actccaacgc cattgttgaa
    21001 gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa aactgattcc gattacctca
    21061 caaaaccgta ttccactgct gcaaaacggc actttcgatt ttgaatgtgg ttctaccacc
    21121 aacaacgtcg aacgccaaaa acaggcggct ttctctgata ctattttcgt ggtcggtacg
    21181 cgcctgctga ccaaaaagag cggcgatatc aaagattttg ccgacctgaa aggcaaagcc
    21241 gtagtcgtca cttccggcac tacttctgaa gttttgctca acaaactgaa tgaagagcaa
    21301 aaaatgaata tgcgcatcat tagcgccaaa gatcacggtg actctttccg taccctggaa
    21361 agcggtcgtg ccgttgcctt tatgatggat gatgccctgc tggccggtga acgtgcgaaa
    21421 gcgaagaaac cagacaactg ggatatcgtc ggcaagccgc agtctcagga agcctacggt
    21481 tgcatgttgc gtaaagatga tccgcagttc aaaaagctga tggatgacac tatcgctcag
    21541 gtgcagactt ccggtgaagc ggaaaaatgg tttgataaat ggttcaaaaa tccaattccg
    21601 ccgaaaaacc tgaacatgaa tttcgaactg tcagacgaaa tgaaagcact gttcaaagaa
    21661 ccgaatgaca aagcactgaa ctaattacaa gaaccagggg cggaaattcc agccctctcg
    21721 attgttacgt agcacggaca gactatacgc ctgatggtcg ttccccatcg ggcctgaaaa
    21781 ccgcaatacg ctgggtaaca atcttcgagg gtagcagtta acgctgctac cctctttttt
    21841 ctggagtaga tttatgtcta tagactggaa ctggggtatt tttttacaac aagccccgtt
    21901 cggcaacacc acctatcttg gttggatctg gagtggtttt caggtgacga tcgctttgtc
    21961 gatctgcgcc tggattatcg ctttcctcgt cggttcattt ttcggcattt tacgtaccgt
    22021 tccaaaccgt tttctctccg gtctgggtac gttgtatgtc gaactgttcc gcaacgtgcc
    22081 gttgattgtg caattcttta cctggtatct ggtgatcccg gagttgctgc cagagaaaat
    22141 cggtatgtgg tttaaggccg agctggatcc caatattcag tttttccttt catccatgct
    22201 ctgcctgggg ctgtttaccg ccgcccgtgt ttgcgaacag gtacgcgccg ccattcagtc
    22261 gttgccgcgt gggcaaaaaa atgccgcgct ggcgatgggc ctgacgctac cgcaggctta
    22321 tcgatacgta ctgctgccta atgcttatcg cgttatcgtc ccgccgatga cttccgagat
    22381 gatgaacctg gtgaaaaact ccgccatcgc ctcgactatt ggtctggtgg atatggcggc
    22441 gcaggcgggt aaattgcttg attactcagc ccacgcctgg gagtcgttta ccgctattac
    22501 gctggcgtat gtgttgatta acgcggttat catgctggta atgacgctgg ttgaacgtaa
    22561 agttcgcctg cctggcaaca tggggggcaa ataatgtacg agtttgactg gagttccatt
    22621 gtcccttccc tgccatatct gctcgacggg ctggtgatca cgctgaaaat caccgtcacg
    22681 gcagtcgtga ttggtatttt gtggggcacc atgctggcgg tgatgcgctt gtccagcttt
    22741 gcgcccgtag cgtggtttgc caaagcctac gttaacgtat tccgctcgat tcctttagtt
    22801 atggttttgc tgtggtttta cctgatcgtg ccaggttttc tgcaaaacgt gctgggatta
    22861 tcaccaaaaa atgatatccg tttaatttcg gcgatggtgg cgttttcaat gtttgaagcg
    22921 gcctactatt cagagattat ccgtgctggt attcaaagta tttctcgtgg tcagtcgagc
    22981 gccgcgctgg cgttgggaat gactcactgg cagtcgatga aactgattat tctgccgcag
    23041 gcgttccgcg cgatggtgcc gcttctgctc actcagggca tcgtactgtt ccaggatacc
    23101 tcactggtgt atgtgttatc tctggccgat ttcttccgta ccgcctcaac cattggtgag
    23161 cgtgatggta cgcaggttga gatgatccta tttgccggat ttgtttattt cgttattagt
    23221 cttagcgcgt cgttgttggt cagctacttg aaaagaagga cagcataatg attactctga
    23281 aaaatgtttc aaaatggtat ggtcactttc aggtgctgac cgactgctca accgaagtga
    23341 aaaaaggcga agtggtggtg gtttgcggcc cgtctggttc cggcaaatca acgctgatta
    23401 aaaccgtcaa cggcctcgaa ccagtgcagc aaggtgaaat caccgtcgat ggtatcgtgg
    23461 ttaatgacaa gaaaaccgat ctggcaaagc tgcgttcccg cgtcgggatg gtgttccagc
    23521 attttgagtt gttcccccat ttgtcgatta ttgaaaacct gactctggcg caggtgaaag
    23581 tgcttaaacg cgataaagcg cccgctcgtg aaaaagcatt gaaattgctg gagcgcgtgg
    23641 gactttccgc acatgccaat aaatttccgg cgcagctttc cggcggtcag cagcagcgtg
    23701 tggcaatcgc tcgcgcgttg tgtatggatc ctattgcgat gctgttcgac gaaccgacat
    23761 cggcgctgga tccggagatg atcaacgaag tgctggacgt gatggtggaa ctggcgaacg
    23821 aaggaatgac catgatggtg gtgacccacg aaatgggctt tgcccgcaaa gtggcgaatc
    23881 gggtgatctt tatggacgag ggtaaaattg tcgaagactc gccgaaagac gctttcttcg
    23941 atgatccgaa atcggaccgc gcgaaagact tcctcgcgaa aatcctgcat taatcactct
    24001 ggcgcgcata cccaagtgcg cgccatctca cgctttatcg tttcgcccct cgtatctgtt
    24061 acatcctggc gttacccttg tcgcaaagaa gcacacaaca aggagcaaca atggcactgc
    24121 caattctgtt agattgcgac ccaggtcatg acgacgctat cgcaatagtt ctcgccctcg
    24181 cctcaccaga gcttgatgtc aaagcaatta cgtcttccgc cggaaaccag acaccagaaa
    24241 aaaccttacg caatgttctg cgtatgctga ccttgcttaa tcgcaccgat attccggtag
    24301 caggcggcgc ggtaaaaccg ttaatgcgtg agttgattat cgcggacaat gtgcacggcg
    24361 aaagcggtct ggatggcccg gcattaccgg aaccgacgtt cgcaccgcaa aactgtacgg
    24421 cggtagagct gatggcgaaa acgctgcgcg aaagtgcgga acctgtcacg attgtgtcta
    24481 ccggaccgca aactaacgtt gccttgctgc tcaatagcca tccggaactg catagcaaaa
    24541 ttgcccgtat cgtgattatg ggcggcgcaa tggggcttgg taactggacg cctgcggctg
    24601 aatttaatat ttacgttgac ccggaagcgg cagaaattgt cttccagtca gggatcccag
    24661 tggtgatggc cggtctggat gttactcaca aagcacaaat ccacgttgaa gacaccgagc
    24721 gtttccgcgc gattggcaac cctgtttcaa ccattgttgc cgaactgctg gatttcttcc
    24781 tcgaatatca taaagacgaa aaatggggct ttgtcggtgc accgctgcat gacccatgca
    24841 ccatcgcctg gctgttgaaa cccgagttgt ttaccactgt tgagcgctgg gttggcgtgg
    24901 aaacacaggg gaaatatagc cagggtatga cggttgttga ttattattat ctgactggca
    24961 ataaaccgaa tgccactgta atggtcgatg ttgatcgtca gggctttgtt gatttactgg
    25021 ctgatcgtct gaaattttac gcttaacaca cgtcattgat taagatcacc acgcgttacc
    25081 tgggtggtct taatctgttt atctcttttt ctttcaagat ataaatgcaa tggataatgc
    25141 agaactcgcc attggtatcg atctcggcac taccaatagt ttaattgccg tctggaaaga
    25201 cggtgccgcg caattaattc caaataagtt cggtgaatat ttaacaccat ccataattag
    25261 catggatgaa aataatcata ttttagtcgg aaaaccggct gtatcacggc gtacttcgca
    25321 tccggataaa acggcagcgt tatttaaacg tgcaatgggc agtaatacca actggcggtt
    25381 aggcagcgac acatttaacg cgccagaact gtcctctttg gtattacgct cattaaaaga
    25441 agatgccgaa gaatttctgc aacgtccgat taaagatgtg gtgatctccg ttccggctta
    25501 tttcagcgat gaacaacgca agcatacccg tttagcagcg gagttagccg ggttaaatgc
    25561 ggtacgctta attaatgaac ccacagcagc tgcgatggcg tatggcctgc atacccaaca
    25621 aaatacccgt tcgctggtgt ttgatctcgg tggcggcacg tttgacgtta cggtgcttga
    25681 gtacgccacg ccggtgattg aagttcacgc ctccgctggc gacaactttc ttggtggcga
    25741 agattttacc catatgctgg tcgatgaggt tttaaaacgc gcggatgtcg ccaggaccac
    25801 gcttaacgag agtgaactgg gagccttgta cgcctgtgtg gaagcggcaa aatgtagcaa
    25861 tcaatcgcca ttgcacattc gctggcagta ccaggatgaa acgcgggagt gcgaatttta
    25921 cgagaacgaa ctggaagatt tgtggttgcc gctgctcaat cgcttgcgag tgccgattga
    25981 acaggcgttg cgcgatgcac gtctgaagcc gagtcaaatc gacagtctgg tgctggttgg
    26041 cggcgcgtca caaatgccgc tggtgcagcg aatcgccgtg cgtctgtttg gcaaattacc
    26101 gtatcaaagt tacgacccga gcaccattgt cgcgctgggc gcagcaatcc aggccgcctg
    26161 ccgcttacgc agtgaagata ttgaagaggt gatcctcact gatatttgcc cttactcgtt
    26221 gggcgttgaa gttaaccgcc agggcgtttc cggcattttc tcgccaatta ttgaacgaaa
    26281 caccacagta cccgtgtcgc gtgtagaaac ttactcgacc atgcacccgg aacaggattc
    26341 aattacggtt aacgtctatc agggagaaaa ccacaaagtt aaaaataaca ttctggttga
    26401 atccttcgat gtgccgttga agaaaaccgg ggcttatcag tcgattgata ttcgctttag
    26461 ttatgatatc aacggcttgc ttgaagttga cgtgcttctg gaagatggca gcgttaagtc
    26521 cagagtcatt aatcatagcc cggtaacatt gagcgcgcag cagattgaag agagtcggac
    26581 gcggttatcc gcattgaaaa tttatccgcg cgatatgctc atcaaccgca cctttaaggc
    26641 caaactggaa gagttgtggg cgcgggcgct gggtgacgag cgagaagaga ttggccgggt
    26701 gatcaccgat tttgatgcgg cgttgcagtc aaacgatatg gcccgcgtcg atgaggtcag
    26761 gcggcgggcg agcgtttatt tagccattga gacctcataa tcacggctgc cggatgcggc
    26821 gtaaatgctg catccggcat cggcgcaaaa aacacatcgt caactaaaca acatcgcgat
    26881 tttgccgaga gcataaccta taaccagcac cgttaaactg attttgataa tgctggtaat
    26941 gctctgtggt ttttcaccct catcattttt ttcttcatgg ttttcaggtg ttggctcatc
    27001 gacatcttca tcctccggct cgagttttac tttcgtcaac agcatccggg tgataatggc
    27061 ctctaatgga tgagcatctc cgccttccag ccttgtatta agacgggcca gcatctcttg
    27121 tttcggaaac gcttcaagta ctacctgttc cgctacccga tacaaatcca gtaacggtga
    27181 atcttcttct gtctcgctac cgactctcga tttccaccac gcccatgctt tgcgtttgaa
    27241 gaataacgac tcaataaacg attgacctaa cggcgataat tttccgctat gtaaaatttc
    27301 cgcccataac agccgcgtcc gggcgctctc accaggaaca tatttcagcg gttgaccttg
    27361 cgcatctacc atgctccgcg cttcgataac catcactaat aacgcgaaat aatccgggca
    27421 atgacgcttg ctccactgca acaacaaatt atcagcttgt gtagatgggt aactttcccg
    27481 gtacgcgcag agatcggcaa gcaggctttc cccctcgccg cagtacaacc tttgagcaca
    27541 caagtaatat cgggcatctt cgctttctgg ttcttccatt tgccactgtt tggctacagg
    27601 aatgagttcc gccatacctg tttgcaccga gctgtaccag cgcaacagtt tgcgatgtaa
    27661 ctttgcatca tcaggaatga accacggacc ttccatcgcc aaaaacgcag tgacgtagtc
    27721 aggatggtaa cggcagcaac gttccagcgc gaagtaatac tcaacagtct ggttctgcaa
    27781 cgcaacggga tattccagaa ggctaaggaa atcgaaacaa tcaccagcct gaatggcctc
    27841 aagaaaagcc tccctttctt cctcatccat tccttcgctg tcattaaacg aatgccagtt
    27901 caaacgctct gccagcaaag aagcacaatt taatgaaaga tatcgcgctt ctatagcgat
    27961 tgcacacagc ggccagcgta attgatcgac atcctccatg ttccaggtat ttaattgctg
    28021 aataaatttc tgccaggcgg agggctgaaa acgatcactt tctgaatcca gtaatgtcct
    28081 gaatgcacgc agtatttcat gttcagcggc ggcatctttt tcttcatcat ctgcttcctc
    28141 gacaggattt accgccagtc gcagcgcctc ttcataggcc tggcgtaatg ctttaaaccc
    28201 ctgcggatcg gtttcgggat gacataacgg taagcgagca agataagcct ggcggataat
    28261 gtctatttgc gtcgtgcttt caatttcgag gatttgccaa catgttttca taggtcgctc
    28321 acatccttaa gcgttaccgg gcattcaggc tggacagtta atggctgcca gggaagcgtg
    28381 gcatagaaac gatcgcttcc cgctgccagc tcttcgaaca tccgactccc ggcgctatcg
    28441 tatttgtaca gggttagcgc ctcgcgtagc gaagtgttgt cttcgcactg agaagattgc
    28501 cagtagagac gtcctaacga ataggcggaa aaatagtgca tccagctgtg atagtaatgg
    28561 tgagctcgta catagacgca cgattgcagc cataaactct cttcttccgt cagccagttg
    28621 ttgagcacac cgacccgtga cagaaacccc attcgaacat aatcccaggc tttgattccc
    28681 ccttctccac agcacatagc ggtttgggca acgaattggg tataaatcaa ccctctctcc
    28741 gagccaccag caattaatgc tttccactct tcaggggaag agcgatgcca tgtcagatat
    28801 aagccagcta aatcattggc atgaccatca tcagtcatct gataaatagt ctgaattaaa
    28861 tcatcacgat tatttattcc ccaggatgat gagagatcga attcttttat cccggtatac
    28921 atttttggcg agtcatgcgc gggtttgcaa ccctgattaa gtatttccat gggcgcagaa
    28981 agcgcaaaaa gaagatattg ttcttcctta ttcatttgtt aataatccct taag
//
LOCUS       DAZGTR010000053        28292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGTR010000053 DAZGTR010000000
VERSION     DAZGTR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.053"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(93..1490)
                     /gene="ccp"
                     /locus_tag="ACHLCY_002521"
     CDS             complement(93..1490)
                     /gene="ccp"
                     /locus_tag="ACHLCY_002521"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP0369756.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1895..3544
                     /locus_tag="ACHLCY_002522"
     CDS             1895..3544
                     /locus_tag="ACHLCY_002522"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0369757.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESSREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3595..4197)
                     /locus_tag="ACHLCY_002523"
     CDS             complement(3595..4197)
                     /locus_tag="ACHLCY_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP0369758.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4717..5616
                     /locus_tag="ACHLCY_002524"
     CDS             4717..5616
                     /locus_tag="ACHLCY_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0369759.1"
                     /translation="MDKIHAMQLFIKVAELESFSHAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5665..6678
                     /gene="yhjD"
                     /locus_tag="ACHLCY_002525"
     CDS             5665..6678
                     /gene="yhjD"
                     /locus_tag="ACHLCY_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP0369760.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7089..8411
                     /locus_tag="ACHLCY_002526"
     CDS             7089..8411
                     /locus_tag="ACHLCY_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0369761.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGSLLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8645..10705)
                     /locus_tag="ACHLCY_002527"
     CDS             complement(8645..10705)
                     /locus_tag="ACHLCY_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0369762.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10775..11542)
                     /gene="pdeH"
                     /locus_tag="ACHLCY_002528"
     CDS             complement(10775..11542)
                     /gene="pdeH"
                     /locus_tag="ACHLCY_002528"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP0369763.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11774..12703
                     /gene="kdgK"
                     /locus_tag="ACHLCY_002529"
     CDS             11774..12703
                     /gene="kdgK"
                     /locus_tag="ACHLCY_002529"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP0369764.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12799..14295)
                     /locus_tag="ACHLCY_002530"
     CDS             complement(12799..14295)
                     /locus_tag="ACHLCY_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0369765.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14516..15802)
                     /gene="dctA"
                     /locus_tag="ACHLCY_002531"
     CDS             complement(14516..15802)
                     /gene="dctA"
                     /locus_tag="ACHLCY_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP0369766.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15985..17973)
                     /gene="hmsP"
                     /locus_tag="ACHLCY_002532"
     CDS             complement(15985..17973)
                     /gene="hmsP"
                     /locus_tag="ACHLCY_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP0369767.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18055..21528)
                     /gene="bcsC"
                     /locus_tag="ACHLCY_002533"
     CDS             complement(18055..21528)
                     /gene="bcsC"
                     /locus_tag="ACHLCY_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP0369768.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21510..22616)
                     /gene="bcsZ"
                     /locus_tag="ACHLCY_002534"
     CDS             complement(21510..22616)
                     /gene="bcsZ"
                     /locus_tag="ACHLCY_002534"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP0369769.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDIWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22623..24962)
                     /gene="bcsB"
                     /locus_tag="ACHLCY_002535"
                     /pseudo
     CDS             complement(22623..24962)
                     /gene="bcsB"
                     /locus_tag="ACHLCY_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            complement(24973..27591)
                     /gene="bcsA"
                     /locus_tag="ACHLCY_002536"
     CDS             complement(24973..27591)
                     /gene="bcsA"
                     /locus_tag="ACHLCY_002536"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP0369770.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27588..>28292)
                     /gene="bcsQ"
                     /locus_tag="ACHLCY_002537"
     CDS             complement(27588..>28292)
                     /gene="bcsQ"
                     /locus_tag="ACHLCY_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP0369771.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINNFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSAS"
BASE COUNT         6641 a         7845 c         7160 g         6646 t
ORIGIN      
        1 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
       61 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      121 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      181 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      241 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      301 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      361 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      421 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      481 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      541 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      601 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
      661 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
      721 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
      781 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
      841 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
      901 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
      961 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1021 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1081 cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1141 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1201 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1261 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1321 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1381 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1441 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1501 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1561 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1621 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     1681 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     1741 tttttataaa ttccattgat attagtgcgt tatatttttt gacaagttaa taaacgtgat
     1801 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     1861 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     1921 taatccagac gaactgatga tcgaggtcga tctctgctat gaactggacc cgtatgaatt
     1981 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2041 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2101 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2161 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2221 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2281 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2341 cattccgtgg tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt
     2401 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtagtcg
     2461 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2521 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2581 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     2641 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     2701 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttattggg acgatcgcga
     2761 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     2821 gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc tcaggttggg attactcttc
     2881 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     2941 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3001 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3061 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3121 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3181 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3241 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaatggct gggcaccgtt
     3301 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3361 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3421 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3481 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3541 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3601 ggagatattt aacatcattg ccgcctgggt gcgatttttc acttccagac ggcgatacag
     3661 ggattccaga tgcgctttta ccgttccggt actgatattc agcgctctgc cgatctcctt
     3721 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     3781 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     3841 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     3901 ggccagcaca ttaaaatgga taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     3961 gattaacacc ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa
     4021 ctcaccatcc tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg
     4081 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa
     4141 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4201 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4261 aaaaataaga attcgccatt tggcggtgac cattctacag agatgacgcg tagaaaatag
     4321 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     4381 aacaattttg aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact
     4441 ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa
     4501 cacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc
     4561 acctttctca gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga
     4621 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     4681 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     4741 ttgttcatca aagtcgcgga gctggaaagt ttttcccacg cggcggattt ctttgctttg
     4801 ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     4861 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga
     4921 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     4981 agtatcagcg gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg
     5041 ccgcgcctgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5101 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta
     5161 ccggaagatg gcgttatcgc ccgtcccctc ggcaaactga ccatggtcaa ctgtgccagt
     5221 ccgcactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     5281 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     5341 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     5401 ctcgccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     5461 gccctgcgtg ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc
     5521 ctttcgctgg tttacccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     5581 tggctggctg gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac
     5641 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     5701 gatctggagc acgagccgat taaacagttg gataatagcg agaaaggcgg caaagttagc
     5761 caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg
     5821 cacctgattc gtgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     5881 atcacctatt tctcattttt gtcgatgatc ccgattttga tggtgtcgtt tgccgcaggg
     5941 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6001 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6061 cagcgtacga ctgtagggct tgtcggcctg gcggtggcac tttattccgg catcaactgg
     6121 atgggtaacc tgcgtgaagc gattcgtgcc cagtcgcgcg atgtctggga acgctcgccg
     6181 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcact gattggtttg
     6241 ttaattgcgc tgattgtgac gctgtcgatc acctcggttg ccggttcggc gcagcaaatg
     6301 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     6361 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg
     6421 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt
     6481 tttgaagtga ttaaaatcgt gatgacctac accttgccat cgctgatgaa atccccctct
     6541 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     6601 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgcca
     6661 gggaaaacgc agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat
     6721 tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata
     6781 atgcgcacat aacctcttga aactcttccc cagagcctct tcagccatct atttgggagc
     6841 aaacaatttc attccaactc ataaccccag catataaatc cagttggtaa cttttattta
     6901 acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa
     6961 atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt
     7021 acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag
     7081 aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccgatca
     7141 actcgcgtaa taaagtcctt gtcgcctctc tcattggcac ggccattgag ttcttcgact
     7201 tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg
     7261 atccaacggc agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc
     7321 ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg
     7381 tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccgggctatg
     7441 ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt cagggtctgg
     7501 gcttaggcgg tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca
     7561 aacgtgcact gtatggctcc ttcccacagc tgggcgcacc gattggcttc ttctttgcca
     7621 acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg agctggggct
     7681 ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt
     7741 cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc
     7801 cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcattatgc
     7861 tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttc agtaccgccg
     7921 ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt gtggatgctg atgatggcag
     7981 ttattggttt tggcgtgatg gtgccagtcg ccggattact ggctgatgcc tttggtcgcc
     8041 gtaaaagcat ggtaatcatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc
     8101 cgctgctcgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc
     8161 tgatgggact gaccttcggg ccgatgggct cgctgttacc agagctgttc ccgacagaag
     8221 tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg
     8281 caccatatat cgcagcctgg ttgcaggcta actacgggtt aggtgcggtg gggttatatc
     8341 tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc
     8401 agtcgttgta agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag
     8461 agggagaaaa gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg
     8521 aacggaaaag cggtactcgt tgtttcaaca cgcacaaaac agtcccctcg cccctttggg
     8581 gagagggtta gggtgagggg aacacgctgc tcccgaactc accagcaaac acaatccaac
     8641 ctcatcactt cttcatctgc gacaatatcg tccgacactg attagcctcc ccttcggaag
     8701 gtgagatcag tgccagtaac gccgccgccg gtgttaccag cgttgccagt gccgcagcaa
     8761 cagcaccacg ggcaatcaac ggcccggctt tcaccccagc ctgcggattt ttaaacgtcc
     8821 cacgcacata cagcggcgaa cgcagtgtga taatccgaat tcctttactc tccggatcaa
     8881 tagtcaaatc cagctgttcg gaagcaaaac ttgccgtgcc ggtaacgtta atcaacgcgt
     8941 tctcagtatc gaaagcaaag atctgcgggc gcgccacgcc gttggcaata tccagattcg
     9001 ccgccgcgca gttcacccgc acctcatcat caccaaatat cgcaccgaca atgtagttgc
     9061 cgacattcag cccaacaatc tccatcaggt tgcggctcac cagcccgtca ttcatcaaca
     9121 gtttcaggtt gccgttactg ttgcctaaaa gtgccgccac cgagttaccg ctaccgcgta
     9181 gttccgcgtc accgttcatt tcccccagcg tcttctgcat cagttccaca tcgggcatca
     9241 gttctttcag tttcagtcga cgagcctgaa tatctgcccg cccctgcatc ggctttttat
     9301 cgccttccag atgaatattc gccgcaatgc tgccgcccgc catgccaaat ttcagcggtt
     9361 gcaggcgcag gtcagcattt ttgaggatga tatgagtaga aagatcgcta atcggcaggc
     9421 tactgccatg ctcaatgcgc cgccctttga agcgaacatc ggcatccata acgtcccatt
     9481 tatcggtttc gaagcggtca taaggcagca ctttgcccgc aggctgaacg cttttttcgc
     9541 ccttcttctg ttcagaccgt ttcgactttt ctgccccttt cccggagtca acgccaatta
     9601 acggtcccag gtccgccagc cgcaattgcc gcgactcgac atcaccttcc agttttggtc
     9661 gtggctttcc ggtggtgtag accagagaac cgtggatatc gctatcgcca attcgcccat
     9721 taaaaccgcg ataatcaaag accgacgatt tttcagtgtc gattttcgct accagccgac
     9781 catccgtttc aaacggcggg gtatcgggca gcagaacgcc cgtcagttca tagagatcac
     9841 ccagtgaatc gccagaaaat ttaagccgta aatcgacacc gcccatcttc attgggtcat
     9901 tcacgacgcc atcaaaagca acacgggtgt taccagagcg gaaatcagcc tgtaccggaa
     9961 acggcgtccc ttcgccacgc agcgccagca taccgcctat ttttcccgta cccgtgagcg
    10021 gttcaccgtt ataacgtccc tgcgccttca ggccaaaaac gtaatcgccc accttttctt
    10081 tatccgcttt acctttcgat ccagtaactt cgctgaacgg cagcggcttg cctaacggat
    10141 caacaaaaat ctccagatcc gctttgctta ctttgtcatc aatggcgatc cgcccttgat
    10201 cgaaaagaat attatccagc cgaaacgacc atgccgacgg ctttgcattc gcgtctttgt
    10261 tatcatcgtt ggcgagatta aacgtccagt tattgttctt ttcggagagg cgaatcaggc
    10321 gcgcgtcggg cttttcgagc ttgatccacg gcagccagac cgttttggtc agcagcgcca
    10381 gcggggccag cgttgcctct acgcgtggca aatgcaccat cgtgacttcg ggaatatccg
    10441 gtggattgcc aagaatgatg tcttccgcgt gtacatgggg ccacggcacc cagctgcgcc
    10501 agccagtttc ttgtttttgc cgctcccaca ccacgcccag atcgccacgg atagcgaacg
    10561 gacgattcaa ctccgcagag actttctggt tgatggtcgg tttgagtcga ttccagtcaa
    10621 aagttgcaat caaaatgatc gccacgacaa tcaacaacaa gaaagccact gaaatcgcag
    10681 cggttatttt gcctgccttg ctcatcgttc gcttcctcat attcttcctg tgccagtcct
    10741 aaagatagtc cagccaggcg gaaaatgagg cagcttatag cgccagaacc gccgtattca
    10801 gcgtttctat cggtgccggg cgtgaaagaa accagccttg tgcggcgaat gcgggcgagt
    10861 tctgaacatc acgccactct tccggcgttt ctacgccctc gacaatcacc ccgcgacaat
    10921 agcgattcat tagatgtaaa agctgagaaa agagtgtgcg tccttccggc gactgacgca
    10981 gcatcacaaa cagttctcgc gcgattttga tgtagtcata acgcacttca cttagtgcag
    11041 agaaatttgc catcccggta ccaaaatcat ccagccacag cgggccaaat tcacacatcg
    11101 aggcaaaggt tgaatctttc ggcagacgga tatgctccac cagttcgaaa cgcagccagg
    11161 gaagacgctc aatctggcgc aggatttttg gttgctgacg cagggcgatg agcgtagggc
    11221 catcaatatt gaccgatgcc agcaggccgt gctctataaa gaagtcggct ttttgcgcca
    11281 gcaaatcaat ctgctctttc acaacctcca tccgatggct gacggtgatt tcagtaaaat
    11341 agcgatccgg cggcaggcgt tgcgaagggt tcaagggatg cgtgaccacc gttaatagct
    11401 ccacggccat taaccgtccg catgtttgat agatcggctg ccaggtgtaa gcacgctcac
    11461 actgcaacca aaaacgccgc tcctgcaagc tctcgatgct tgcttcaggg ttgcttattc
    11521 gctggataac ctgccttatc atcaaagatg tcctgtgttt aaaagtgatg ctgcccggac
    11581 tcgacaaaga ttatcggcgc gcaagggcag aactttaccg cctggccggg gtaaatttcc
    11641 tcactccaca acggaatctt tcaggaatac gttctggctc aaaaaaataa tggaacactg
    11701 ttttaatatg gttgaccagc aaaccaccac agcgcaaact aacgctaatt ttttacagat
    11761 caggttcacg actatgtcca aaaagattgc cgtgattggc gaatgcatga ttgagctttc
    11821 cgagaaaggc gcggacgtta agcgcggttt cggcggcgat accctgaaca cttccgtcta
    11881 tatcgcccgt caggtcgatc ctgcggcatt aaccgttcat tacgtaacgg cgctgggaac
    11941 ggacagtttt agccagcaaa tgctggacgc ctggcacggc gagaacgttg atacttccct
    12001 gacccaacgg atggaaaacc gtctgccggg cctttactac attgaaaccg acagcaccgg
    12061 cgagcgtacg ttctactact ggcggaacga agccgccgcc aaattctggc tggagagtga
    12121 gcagtctgcg gcgatttgcg aagagctggc gaatttcgat tatctctacc tgagcgggat
    12181 tagcctggcg atcttaagcc cgaccagccg cgaaaagctg ctttccctgc tgcgcgaatg
    12241 ccgcgccaac ggcggaaaag tgattttcga caataactat cgtccgcgcc tgtgggccag
    12301 caaagaagag acacagcagg tgtaccaaca aatgctggaa tgcacggata tcgccttcct
    12361 gacgctggac gatgaagacg cgctgtgggg tcaacagccg gtggaagacg tcattgcgcg
    12421 cacccataac gcgggcgtga aagaagtggt ggtgaaacgc ggggcggatt cttgcctggt
    12481 gtccattgct ggcgaagggt tagtggatgt tccggcggtg aaactgccga aagaaaaagt
    12541 gatcgatacc accgcagctg gcgactcttt cagcgccggt tatctggcgg tacgtttgac
    12601 gggcggcagt gcggaaaacg cggcgaaacg cgggcacctg accgcaagta ccgttattca
    12661 gtatcgcggc gcgattatcc cgcgtgaggc gatgccagcg taaacgcagc atatgcaaaa
    12721 ggctggataa agtgttaccc ctctatccag ctcatccata aatacctgat ggttgtgcta
    12781 actcatcacc atcaggcatt actgtgcagg tggaatatcc gtcacttcag gatgtacgtc
    12841 atccgtggcg acagaggtgg cggcagcagc agtcgatggg gccatgattt gatcccaggc
    12901 cgcctgcaac gctttcatgt taaattccgg ctcgcctttc ggctgcagca gtattaacgc
    12961 catatcattc gacaactgct gacgcagatc ctgatttaac atctccaccg tcaggctgtt
    13021 aaggaaatcc tgccgcagtt tctgatactg ctccggcgcg atatcgacga cctgattttg
    13081 caacgaacgc atccgctgac ccatcagaat atcggtatca gcccgcgcat aggcggcaaa
    13141 cagtttctgc agctccagtt tcttttgcgc cactaacgca ttgaactctt cttccggcag
    13201 acctttatcg cgaaccttcg ccagttcacg cgccaccaga ttaaggttgc tgttcagctt
    13261 gtcgtttggt gattcgatgt tgatggcaca ctgcgcacgc agatacagca cacggcagtc
    13321 aaatccaaga ccgatgtctt tgctgttact ggcgcttaac gcttgctgaa catgccagaa
    13381 cagcgcctcg cgggccaggt ccgcacgcca gtagcgcagc agtgcggctg attcacgaat
    13441 cggctgccac ggcgtatccc acatgataga taaccggtcc tgacgcaccg cgtcagtcat
    13501 aatactcacc gcttccgcac gcagcggaga aagcgtcggc accggagctg gcgtttcacg
    13561 tttgcctttc agttcgccaa acgttttgtt tatttggtcg acaaccgagc gcgcatccac
    13621 gtttcccacc accagtagcg tcattgcatc cggggtgtac catttctgat agaaatcttt
    13681 aattttttcc gcttcaacgg gttgtttcag cggatcggca ggatcgtgac ctaacaaggt
    13741 tgaccctttc agacgatagc gccaccagcc ctctttagta tcggcaggcc aggttgctac
    13801 catgtcctga ctttgcagcg cgtggttgat ggtttctggt gtgatggtca atttgccagt
    13861 ggcatttgcc agataagaga gcgcttcttt cagcaaatcg ttacggttat tgggcaaact
    13921 cagattaaac agcgtggtgt cataagagac aattaccggc ggcatcgggc gtttagggtc
    13981 gatcccctgc tgccacaatg aacgcgcctg tgctgcgtca aggccaccgc tttgcgttag
    14041 cgcaatacga gggatggcgt gactgtaacc gctctgttgt gtactttcgg cgagcgaacc
    14101 ggtattaacc agcaggcgaa tttcaacacg atcgctggga cgctgggggg tggtcagcac
    14161 ttgccactgt aaaccgttgg aaagcgtccc ctgttgccat gctggatcag gctggagcgc
    14221 atctgcctgc acatagccag cagtggccat catcagcaaa ccgcccgcta aaagtcgaat
    14281 ttttgtgccc tgcatgtgaa cccctgatca actatcctgg taataaaaaa actgcccgtt
    14341 gtcggtcagt ctaaatgacg ttaaaaacac ttcgtgttag accgcaagaa catgaaaatg
    14401 tcacggaaga agtgaaataa acctgaactc acccaggtgg taagttcagg cacaggggtc
    14461 aattatgcgc aaacacccgc actcggggaa gggagtgcgg gcataagtga tgagattaag
    14521 aggataattc gtgcgttttg ccatccggcg cacgattatt cagcacatcg tccagttttt
    14581 tgtggtccag ttctttcacc cacttagcaa cgacaatggt cgctacgccg ttaccgacca
    14641 ggttagtcag cgcacgagct tctgacataa agcggtcgat accgaggatc agcgccagac
    14701 ccgctaccgg caaatggccc accgcagaga gcgtcgccgc cagcacgata aagccactac
    14761 ccgttacccc tgccgcccct ttagaagaaa gcagcaacac gattaacagc gtgatttggt
    14821 ggacgatatc catctgactg ttagtggcct gggcgataaa caccgccgcc attgtcaggt
    14881 atatcgatgt gccatcaagg ttaaacgagt agcctgtcgg gatgaccagc cccaccaccg
    14941 atttgcggca gccgagtttc tccatcttgt cgagcatacg cggcagcgcc gactcggaag
    15001 atgaagtccc cagtacaatc agcagttctt cacggatgta gcggataaat ttgaagatac
    15061 tgaaaccagt cgctttagcg attgaaccca ataccagcac cacaaacagg atacaggtaa
    15121 tgtagaaaca gataatcagc tgccccagtt gcaccagtgt gccgacgccg tatttaccga
    15181 tggtaaacgc cattgccccg aacgcaccaa taggcgccag acgcatgatc atattgatga
    15241 tgccgaagat gacctgcgag aaactttcga tgacgttaaa aatcagttgg cctttgctgc
    15301 ccagacggtg gagcgcaaaa ccaaacagta cggcaaacag cagcacctgc agaatgttac
    15361 cgctggcaaa tgcgccaatg acgctcgccg ggatgacatc cataatgaag gcgacaatgc
    15421 cctggtcttt cgcctgatcg gcgtaaaccg ctaccgcttt cgcatcaagc gttgccggat
    15481 cgacgttcat tccggcacca ggctgcacga cgttaacgat gataagacca ataatcagcg
    15541 cgatggtact gacaatttca aagtaaagca gtgcgactgc gccggtacga ccgaccgcct
    15601 tcatgctttc catacccgca atgcccgtta cgacggtaca aaagatgaca ggagcgatga
    15661 tcatcttaat gagcttaacg aagccgtcgc caagcggttt catttgctcg cctatttcag
    15721 gatagaaatg gccaaggaga ataccaatgg ctatcgctgt caggacctga aagtaaaggc
    15781 ttttaaacag agaggttttc atagggtgtc ctttagtaaa accacaggtc ttgtaaggtt
    15841 atggggtacc tgcggcctta aaataacacc cagacaacat cacagaaatg tacctggatc
    15901 ataattgaaa caaaaaggtt aaaaagtttg agctggctcg cacaaaccag cactttttaa
    15961 agttttgtaa tcagtttggg gtagctactt ttcttccagg taactctctt cgaagatttc
    16021 aatagggagt gggcgagcaa aaaggaagcc ctgggcaata ccaacgcccg cttttgccag
    16081 ccagtcgcgt tgtgcttcag tctccacgcc ttcggcaatc atttgtaagt tcaggctctg
    16141 cgccagcatg ataattgcag caatcatgct gctatcttcc ggcaagcctt caacaaacat
    16201 tttgtcgatt ttcagtacgt cgattggcaa cgatttcata tgctgcagct gacgcagccc
    16261 tgcgtagccc atgccgaaat catccagcgc cacccgaact ccggcattgc gcagcggacg
    16321 gaggattgcc accgcagcat gagggtcgtc aatacgtcgg ctttctgtca cttccagaat
    16381 cagtgttccc ggctgaatgc gatagcgggt taacagttcc agcatatccg ccaccatatt
    16441 tgggtgcatc agttgcagcg cagagaggtt taccgacaag ggcagcataa tgccgcgctc
    16501 ttgccaggct gcaagcaatc gacaggactc ttccagcacc cagtgaccga cggtaaccat
    16561 cagcccacag cactcaatgc gatcgattaa gccatccggc aggtcccaac tgccatccgg
    16621 ttgctggata cgcagtaaca cttccgcact gaccagtttt ccgctggtca tctcgacctg
    16681 tggctgtaac caaatagcaa actgatgatt ttccagtgca ttaaggatat cgctctcttc
    16741 cgtcaaccgc ttctgggcgg cttccatctg ctgcggatca aagaactgaa tctgattctt
    16801 gcctttatgg cgagcggtaa atgccgcaga aatagcgcga ctgtaaagct gttcggcggt
    16861 gagatcgccg tagaacatcg ccacgccaat gctacagtgc ggacggagtt gaatacgttc
    16921 aatcggcagg cgctcgctca tgatagtgag cacttgctga cctaaggtga ttgcgtgcca
    16981 cggttcctgt acaccgttgg caatgacagc aaagtcataa ccgctaatct gcgcgaggat
    17041 catacgtggc gacagtaccg atttgagttt ttccaccagc gtcagcagca gaatttctcg
    17101 ttgcgcctct ttcagcacgc ccgcagtatc acgcagggtt tcacaggtga tgatcatcag
    17161 cgcggtggtt tgtttacgcg cgacaacctg ctccagcatc tccatcagca aggctttgtt
    17221 cggcaaatcc gacaccggga agcgcatcgc attttcgttc tgttcttcat aatggcgctg
    17281 cagcaattgc tggttgaggt tgtaactgcg caccaacata ccgatttcat cgtcctgatg
    17341 cagacgcggt aatgccagtt ggtgaccaac aagctcctgg gctgggatgg cgttaagttc
    17401 gcgagcaata ttgcgtaacg gatgcaaaat caggcggtta atgcaccagc tgatggcgac
    17461 ggtcaggata agcgacaaaa gtaagtaaat ggtcactaac gttgagaggg tgctcatcac
    17521 gaacttatac atacggaagg aatccgcctg tagtaccaga taggcaattg gctgcgggtt
    17581 tgccggacgt tcgagcgagt aaacgcccag cgagatttga accggtagct caaacaggcg
    17641 agtaaccatt accggcaccg ggcgctctgg aataaaactt ttacgcagcg cctggaactg
    17701 gttaggcagc actacatcgg cacggctgac cacgcctgcc ggtttaatgc tggcaagaat
    17761 agcttccgct tcgggaatat cgcctttcaa aatagctgaa gataagggtt cgcggacaga
    17821 gcgagcaatg ctttccagtt gcgtagccgt gttatagcga ttctgttgga ccagatggaa
    17881 cagcaaaacg gtgcaaaaaa taaaaacgaa caccaggaca acggctgcca ccattgccat
    17941 ctgcttgatt gttaacgagc gacttacgcg caaattgtct ccccccggaa atcccagatt
    18001 gccgcccgag tatacccgat cgcagcggca ttaagagagg cgctatctga aaacttacca
    18061 gtcggcgtaa ggtatcagcg gctgcggcgg taaatccatg tcaccctgcc atccggcggc
    18121 ggaataacgt acgtagagca ggaaatggct gggtgcgtaa tctttcgcct gctggatatc
    18181 aattgccgtg ccaacaaacc agttggaagt aacacgtcgt tcaagtaatg cccgcgccgt
    18241 gtagccgaag ccctgactgc tgccgccatc gttggattgc cgcgcagctt cttcctgcca
    18301 gtcggtcggg atcagattca tcagcggata acgcggcatg gttttggtgc gtgaatgcga
    18361 ccacgagcca gacgccccca gctcccacga ccagttttcc gtgcgctccc gccacatcac
    18421 cggtatggca aacgacaggt attcctgcgg actgtagtaa ccgccctgac cgagtgagta
    18481 gccactcaga tctttgtcgt aatgccagat catgttgttc aggccgattg tgacgcggcg
    18541 attgttctgg ttaatgacct tatagtaata gcccgtcatc cagcgcacgc gccagttatc
    18601 ttcgacattt ttaccggtta actggtcgcc actaagcgat gcccagacgc cgtttgcttc
    18661 acctttatcg tagctcagac ttagccccac accgtcggca cgtacgccac cccatttttt
    18721 cccggtattg ctcggggagt ctttttgccc accaaaggcc agcaaagaac tggagatggg
    18781 ccgacggtgg gcgttaacgg tgtaacccag cggcccgata tcatcgctgt aactgatgcc
    18841 gccgaccaca tccaccacgt tgaagcccat cggcgtggta ccgatatccc agctccagac
    18901 gtcatttcgc cagccgaccg ccacgctggc accggaatcc gactggctgc ggttgccgct
    18961 acagtcctgt aatgtacagg tgccccagtt gtcatcccat ttgccatcgg cattagtgga
    19021 gaaactgccg acgttcatat tgacgaaatc actgcgaaag aacatccgcc cgtcagaata
    19081 cggcgcatcc acctgcaaca tggtagtgtg cgctttcaga tcggagtaac caccggtgcc
    19141 gctcgaaccc cagtaatcgt gctcgagggt gacgttaaga tcctgctggc gatagaggtc
    19201 cgccgcatcg ctgcgcacgc cgcgtttcag ccagtcatct ttctcgtcgt tacgggtcag
    19261 tcgggtgaat gtgtcgttat cctgcggacg cgtcgtagtc acaccggatg cgaccatggc
    19321 gtctttgtag gtttccagcg cctgctttgg atcgcccgcc tgcgcttcaa acttcgcacc
    19381 gtcgcgcagc accatcgcgc tttccatcga cggtggctga gattttgcct gcgggatcaa
    19441 cttattaaac gtctgctgcg ccgctgcggt atctccaagc tgcgcctgcg ccagcgccac
    19501 gcgccgctgt gtgttcagcg aggcgttatc ggtagcgggc agtttcgcca gctggctacg
    19561 tgccgccgct gtgtcaccgg cagcaatatc cacttccgtc agccccagaa tggcatcggc
    19621 gttagttggc tccagcgtca ggacattctg atatgcagcg cgggcggcgg tgtaatcacg
    19681 tcgctgctgc gcccagtcag ccagcgtgag gtcaatacgc gtggaaggtg gttgctgacg
    19741 cagcatcgct tccgcttcag cttctttgcc gctttctcgc aggcgattag cggtttccag
    19801 cacctgatcg ctttgcagtc gattaaccag ctcctgaata ttgctgttcc actgcgcgcg
    19861 cggcaggctg ttgatatgcg ccagcgccgc tctgtcctgg tcatgaccag agaggtacag
    19921 cccgtaagcg taaacctgct ccgggtcgtt cggcttctgc tgcgccagat tgcgcattaa
    19981 cgtatcggcc tggctgcgtt gtccggcctg ccagagatcc tgcgaaagtc ggtaagtaat
    20041 ccatacgctg ccggggtcca gcgccagtcg ttgccgctga agtgctgccg cctgcgccca
    20101 tttgccctgg ttttccagtg cctctgcctg ctgtgccaga cggtcgtttt gcaggctgcg
    20161 ttcgatatca tcaatgctac gccgctgact ggcagagagc gaggcgataa acgcttcagc
    20221 tttttctggc gattgctggc ggtaaatatt tgccagcccg cgcacggcgt tagtgttgcc
    20281 gctgtccata cgcaaggtct gctgataata acgttcggcg gcgggataat cttttcgcgc
    20341 catcgccaca tcgcccagcc ccagcactgc ataactgtcg gtgttatcga cattacgcgc
    20401 ctgctggaac aggcgttctg cccggtcagg attattggct ttcagcgcag catcgccctg
    20461 ctggatcgcc agccagtagc ggtttacttt cagcagactg ttccatttgt cgttgttgct
    20521 gctgtgcggg tccagtgcga gggctttttc cagattcgcc actgcattgg cgcgatcgcc
    20581 tttctgagaa tacgcctggc ccagcgcccc cagagcttca ctgtctttcg ggttcgcccg
    20641 caccgcctgt tgtagttcgg gaatggcttt acccgccata ccagagtcca ccgccgctaa
    20701 accttgcgca cgagcgcgga aagcaggatc ggccagctgt ttttgctgtt ctgccagttg
    20761 cgattgcgca gccgccacgc tatcgccatc actaaagatc gagagatatt ttttcagcgc
    20821 cgacacactg gcatcactga cgggcatgtc tttaatctgc ccgtaccaga ttttagaggc
    20881 cccttcgcgc ccggcgttcg attttgccat ctgttccagg acggcaaaac cttcgtcacg
    20941 gcgatcgcta ctaaacagca atagcgccag attgttttgc aggcccgtat tgcccggtgc
    21001 atccgcattg atgcgtttta actgattaat cgcttcgcca cggcgagccg gaattttcgc
    21061 caccgtactc cagtactcga cagcaatgtc accttccggc ggcgcaccgt tgaacagttt
    21121 gttgtaactc gccacagctt cttctgcatg accggtcgtc gcctgcaatc gtgcctgttg
    21181 cagtgcctga cgaccatccg gcgtggaaag tagcatcgta gtccgcgacg atttatacgc
    21241 atttgaactc ggcgctaact gcgacagccg atcgagctgt ttttgcgcgc catcaatatc
    21301 gccctgacgt aacaaagaac ggaaacgggc ggcaacgacg tccgggttat tcggatcaat
    21361 aagttccagc cgatataacg actgttgcac cagatcttca cgatgggtcg cttcgcctaa
    21421 ccgaacttgc tccagcaact gttgctgagc ggttggtgct gcctcgacca tcggcatgac
    21481 ggccagaccg agggaaagcg tgaatatgtt tagtgtgaat ttgcgcattc ctggccccag
    21541 tcaggtaata actcaccttt tgtcgagaag cggaaacggt gttgatccca gccttgtcca
    21601 aacagggtca gcacatagtt gtaataggca tcgctgccgg gaaagttatc ggccacgcgc
    21661 tggcgctgaa cggcctgcgc atcgcggttt tgtaaaaagg gcagcatggc ggcagaaaaa
    21721 ccgaccggtc ctttaccctg cgctttcccc gtagccacat ccactttttc cggcggataa
    21781 ccgtttttct cagtgaatgt cgccatcggt ttaaaccggt tgagcatccg cgctttttgc
    21841 ggatcgctgt caggcatcat gcctacccac atgtaaacgc ggatagcgtc gtagctgctg
    21901 atcaatgttt tttcggcttt tagctgccag cctttgtctt tctcatagcg cacccagtct
    21961 ggcgaaaaac ctttcggggc agtttccagc aataaacgtt gattggtttc gcgcagcgtg
    22021 gtccacggcg cgccaaagcg ggtgaaatac tgcgccagcg tcggcggcag gtagctgggg
    22081 ttaaaacgcc agctgttatc ctcagcaaaa cccactttgc ccggtaacaa catggaaccc
    22141 agcccaggca ccgtcaccac ttcctcccgc gcgatacgtt ttagcaacgc gctgccgatg
    22201 tcagtataac gctgctcttt ccacaaacgc cccgcctcca gcaacgacca ggccatccag
    22261 atatcaccat cggaggccga attgctgtcc agcacttccc acttactgtt ctctttcttg
    22321 ccccacagcc aggcgggcaa acgttctttt aaagaaccct gagcgagatt gttctgcgtc
    22381 cagtcgagaa tattatcgaa agctgcacgg tcgttagccg ccagggcaaa gaacatgccg
    22441 taactttgcc cttcggaggt ggtgattttg cgcgcgtcgc tggggtcgat gacgcgccct
    22501 tcctgactga tgtaatcctt tttaaactgc tcccaggcag gccaggtaca ggctgcctga
    22561 acactaaagg cagccagcag cagcatcgtc acgattccac tacgcaacac attcatcttc
    22621 aattactcgt tatccgggtt aagacgacga cgactaataa ttcgcagcag acgccacagt
    22681 acccatgcca gcaatatcac actgatagcc gccagcaccg ccagcagaat cggatggttt
    22741 gccagcgcat accacacgcg ctcgaaccac ggcagatgac ctacgtaata aacgtcgcca
    22801 acacgtaggc tgttgatacc ggactcgcgg atcaccgcga ccgaaccgaa catggtggcg
    22861 cgtttgccgc tatcgttcac cgcatcgtta agcatttcat aaccgcgtgg gctatctgcc
    22921 aacagcgcaa tcacgctgcg ctggtcgtta tacggcgact ggaagccaat caccgccgcc
    22981 atcgcaccgg aagaggtcag cgttgaccgg gtttctgccg cgcgatcgct ctcgtccggc
    23041 acaatgccgg ggaacggggt ctggcgcatc ggtgttttca cccagctttc ggtcgcctgc
    23101 accaataggt cgatctgctt atcgtctttc agtttgtccg ggataccacc gatgatcatg
    23161 atgtcggcat ctttgccctg aatggtgctg ccatcatcgg tcaccgtcag attaatcgcc
    23221 gggaagcccg tctgtgcgcc gataaaacca acagtattca gcaacgtttc catctgtgct
    23281 tcgttaggcg ctttcggcat cacggtgatg gtttgcgaca gatccgccat ccggctgaat
    23341 gggaagcccg cgttagcaaa ggcgcgtaga tccggcatcg ggatgaagtg gtaatacttc
    23401 gagaagtcga tggtggagtc gtcaccaatc accacatgat tctgcaccgg ctggaaggta
    23461 atacagttat ccaccgaacc gcccggcatc gggttcatat actcaaagtc gaagcgcagc
    23521 tggttggtcg cgcccagttt cagcgccgga atagagacat ctgttttgcc atccagcaaa
    23581 ccttgtaata ccggaatccg cagcagcagg cggttcgcct cctgtttgct gctcagattg
    23641 aaggattgca ggaactggtt attcaggctg atatccatcc gcgaactgtc tttcaccggc
    23701 ggcatggtgt agcggtaatt aatatccata tcaatgccgg tactgcgcat caggtagaga
    23761 tccggcggca ggtttagcga aacgttaatc gctgctggct caagaccgct ggattgtaac
    23821 tgttcttcat aggttttcag ttcgccaaat gtgaccggac gatcggtacg tacccagttc
    23881 ggcgcatcgt acggcttacg cggtagcagc ggtttcactt cattcactac cacgctttca
    23941 ccacggaaca gaatgttacc ctgagcgata cctttcgctg cctgcaacaa gtctttgtcg
    24001 tcacgaccaa acaccaccag cagtttgacg taaggattct gcggatggtt aatcatctca
    24061 atcacagggg cttttaccgc cggatgatcg cgcaggaagt ccggccgttt gtcgttggtt
    24121 gcaaagacaa tggcattacg atccggcagt tggttataga gcaccgggaa gttctgccca
    24181 cgccagccag aacgcgaacc aaaccacgag gcgacaatgg cagaggcttg ttgcagccca
    24241 acatccggcg cacccgcaaa gaccatcggc aaggtgttag tacggttatc gcgcgggtca
    24301 aagaatggca ccgggaagtg tgacaggtca ttcttcacat tcagggtctg ataggtcaga
    24361 tccagtccac tgctccgccc aacatccagc caaagcgtgg tgctggccgg gttttcgcac
    24421 acgtcctgat aatggccgac aaactccagc cgcacacggt tgaagtcggt aataaacagt
    24481 gggttaatgg gcatttgcgc cagcgttttt ttacccaact gttctttggt cactggcagc
    24541 acgcccatca gctcatcatt gagataaacc tttaactgcg actggacagg cagtaacgat
    24601 ggcgatggcg tgtattcgag gttgagcatc gccttcgtca ccacttcatc gctgcgcatg
    24661 ccaaactcaa tgctgccgtt cgggttaatg ccacgtagca ccatgctgcc cggcggcggc
    24721 gcaatttgcg caaaggtcag cttcacatca cgcgaaggac cgttctgcgc cacgactggc
    24781 gcatcagcgc cctgcacgcc cggcatcact tgccccacct gcggattttg ttccgtctgg
    24841 gcggctacag ctggctcagc attgatcagt ggttacgttg ctggcgtcgc ctgcgtcatg
    24901 aaagagggga acgcactcat ccccatagcc actgcacaaa tccagaatag ttttcttttc
    24961 atcgcgttat catcattgtt gagccaaagc ctgatccgat ggttgtgccg tttcgctccg
    25021 ctccgggcgg cgcggaataa acgatacaac ccaggaaacc agagaagtca gcacacggaa
    25081 tatgcccttc accgaagaag gcgcaaactc cgccagatgg cggtagccac ggaagccgag
    25141 cttcagaata tccagcagac tttccagcgg cttatcttcc gggtagctgt cctgccagag
    25201 cgcccatgta tccgcacggg caaacgtaca ctgcacaaaa tcgatatgtt gctgggtggt
    25261 gagcggcatt aatttcagcc caacttcatt acccatcacg cgcgccacct gggtcgggaa
    25321 gacgtattcc tgctgaccgc gtttaagcaa cagattcact ttctgccctt ccagaatctg
    25381 cgcctgaccg ttgatcttga tccccaaacc accgtcggag aaatcctgaa cggtacacga
    25441 gaagaggtga ccatcttcgc gggcaattgc cgcgggcatc gtcatctcca cgcggtgcga
    25501 tcggcgtacc tgtttgcttt ctaccgatac tgcaactgcg ccgccaagaa caatcaggtt
    25561 gtagaatacc cacaccatac tgacgaccac ggtgagcatc tcggttggcg ggccatagaa
    25621 gtagcgccag atgcctaccg caacgcccac caggttgagc aggacaagga agatgtaggg
    25681 ccgcgagatc acccagtcga cgtactcttc ttccaccagt ccacctttgg cggtgacgtt
    25741 aaatttgcct ttgtgcgggt taatcagcgc caccagcgtc ggtggtgcga tataccacgc
    25801 cagcaccgtt tcgtagattt cactccagaa agagtggcga tatttgccct ggatcttgga
    25861 gttggtcagg ctggcatgga tcatatgcgg cagcacgaac agggcgatca tcaacgctgg
    25921 cgcatagatg atgtaggcat gaagcagcag gaacgccagc ggcgcagtca ggaagatcag
    25981 tcgtggaatg cccgacaaga agtggaacat ggcgttgacg tagcacagcc gctgggcgaa
    26041 tttcagccct ttaccggtaa gcgggttatc gagacggaag atttgcacca tcccgcgtgc
    26101 ccagcgaata cgctgaccga tatgcgccga cagactttcg gtcgccagcc ccgccgcctg
    26161 cggaatacgc atatatgcag aggtgtagcc acgacggtgc aggcgcagag acgtgtgcgc
    26221 atcttcggtt accgtttcga cagcaatacc gccaatttca tccaatggtt tgcgacgaat
    26281 tactgcacag gaaccgcaga agaaagtggc gtcccacata tcgttgccat cctgcaccag
    26341 accatagaac agcgtgcctt cgttcggcgt tttacgaaaa cgccccaggt tgcgttcaaa
    26401 cgggtccggc gagaagaagt ggtgcggcgt ctgcatcatc gccagctgtt tctctttcag
    26461 gaaccagccc atggtcattt gcaggaacga tcgcgttggt acgtggtcgc agtcgaaaat
    26521 cgacacgaac tcgcctttgg catatttcag cgcattgttg atgttgcccg ctttcgcatg
    26581 ttcatgagtg gtacgggcga tatatttcac ccccacgttt tgcgcaaact ggcgaaactc
    26641 ttccctaccg ccgtcatcga ggatccagat gttcagcttg tctttcggcc agtcgatacc
    26701 cagcgaggcg taaatagtat ttttcaccac gttgagatct tcgttgtaag tcgggacaaa
    26761 gatatccacc gacggccaca gcgacatatc tttcggcaat ggcaccggct gacgattcag
    26821 cggccatact acctggaagt agccgagcac cagcacaatc cacgcgtacg tttcagcgaa
    26881 gagcagaata agcccgcaca ccaggctgac cggatcgtcc cagttcagcg tagaggtgta
    26941 acgccaccag atataacggc aagaaacggt cagcgacagc acaatcaaca ttagcgccga
    27001 gaagcgcccc ggcatccgcc gtacgatcag cgctaccccc cacagcagca tcaggaagat
    27061 aaactgcgcc agcgggttaa acggctgagt aacgcagatt aacgccagaa tcagcgagaa
    27121 ggtgacgatg ataccgagga tcaaccgccg cgccccggca ctcaaatgac cgagctcttt
    27181 tttctcatcc agatgctgtg ttttatggct aacgcgctca ggcagctcgt tcatccattg
    27241 atggtaacgt ccacgaatat tttgcagacc tgaaaatgcc cgcctgcgcg gtttcggcgt
    27301 ttctttgcgc gatgcaccga tcaataacca gcatgtttga atgagataac ggaccgggtc
    27361 cagcggacgc ggacgcgagg cgttgatatg cggatacagg tttttatgtt ctgcgcgaat
    27421 acgctgccag cgcgggtgct ccagcggaat aaaaatccag gccaggatca tccagaaaca
    27481 gccgagcgtc gcgctgaaag ccgacgcacc gtggcgacga taatcgcgat aacgcccgat
    27541 aagccgcgcg ttgaccggcg ggataagcaa ccaccgggtc aggatactca tgatgcactc
    27601 ccgactggcg ttttcagccc ggaatagttc aacaggcacc agttcgccag cgtcagtatc
    27661 tcttctgccg ccagcgcatc actgcggtat tcaccgactg gttgcttagc cgccaggcat
    27721 tcagccatcg cttcatcacg atgaatgagc atcggcagca atcggcgctg gctttgcaac
    27781 caaagctggt aaatatcgtc ctgaacctga ctgccaatac ggaagttatt aatcaaaata
    27841 tgtgcgccat ccggcagcgc ttgctgatgc aggcggatat ggcagttggc atccacattg
    27901 acgatagcca gcgagtgatc gcacaaactc agcagctggt gggttatctg cgaggcatca
    27961 cgcggtaagt cgattaaaat ccactggtaa cgcccgctgg cttttagttg ctgtaagccg
    28021 gagcaaatat cgctcagccg ggtttgccag tgctgtggat tttcttgttc ttcaatggat
    28081 aactgaccaa aaggcagcaa atcgagctgc gaggtgtagc gcaacccagc gtcacgccag
    28141 tcctggccat ccagcatcgc tctggcccag ccctgacggt gggtaaaatc aacattaaat
    28201 gacaggcgca acaagttgtc cgggcaggca tcgaccacca gaacattttc tcccaacatt
    28261 tgtaatgacc aggctaatgc ggcggtgatg gt
//
LOCUS       DAZGTR010000054        28241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGTR010000054 DAZGTR010000000
VERSION     DAZGTR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.054"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..110
                     /locus_tag="ACHLCY_002538"
     CDS             <1..110
                     /locus_tag="ACHLCY_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369772.1"
                     /translation="HVKTFTKIIFIFSVLVFNDDESKITDAAVNLFIQI"
     gene            124..996
                     /gene="ygcG"
                     /locus_tag="ACHLCY_002539"
     CDS             124..996
                     /gene="ygcG"
                     /locus_tag="ACHLCY_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0369773.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1056..2354)
                     /gene="eno"
                     /locus_tag="ACHLCY_002540"
     CDS             complement(1056..2354)
                     /gene="eno"
                     /locus_tag="ACHLCY_002540"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP0369774.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2442..4079)
                     /gene="pyrG"
                     /locus_tag="ACHLCY_002541"
     CDS             complement(2442..4079)
                     /gene="pyrG"
                     /locus_tag="ACHLCY_002541"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP0369775.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4307..5098)
                     /gene="mazG"
                     /locus_tag="ACHLCY_002542"
     CDS             complement(4307..5098)
                     /gene="mazG"
                     /locus_tag="ACHLCY_002542"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP0369776.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5169..5504)
                     /gene="mazF"
                     /locus_tag="ACHLCY_002543"
     CDS             complement(5169..5504)
                     /gene="mazF"
                     /locus_tag="ACHLCY_002543"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP0369777.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5504..5752)
                     /gene="mazE"
                     /locus_tag="ACHLCY_002544"
     CDS             complement(5504..5752)
                     /gene="mazE"
                     /locus_tag="ACHLCY_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP0369778.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5830..8064)
                     /gene="relA"
                     /locus_tag="ACHLCY_002545"
     CDS             complement(5830..8064)
                     /gene="relA"
                     /locus_tag="ACHLCY_002545"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP0369779.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8112..9412)
                     /gene="rlmD"
                     /locus_tag="ACHLCY_002546"
                     /pseudo
     CDS             complement(8112..9412)
                     /gene="rlmD"
                     /locus_tag="ACHLCY_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
     gene            9469..12225
                     /gene="barA"
                     /locus_tag="ACHLCY_002547"
     CDS             9469..12225
                     /gene="barA"
                     /locus_tag="ACHLCY_002547"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP0369780.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVMNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12457..13797)
                     /gene="gudD"
                     /locus_tag="ACHLCY_002548"
     CDS             complement(12457..13797)
                     /gene="gudD"
                     /locus_tag="ACHLCY_002548"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP0369781.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13818..15158)
                     /gene="gudX"
                     /locus_tag="ACHLCY_002549"
     CDS             complement(13818..15158)
                     /gene="gudX"
                     /locus_tag="ACHLCY_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP0369782.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQVADFDTFGKSA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15160..16512)
                     /gene="gudP"
                     /locus_tag="ACHLCY_002550"
     CDS             complement(15160..16512)
                     /gene="gudP"
                     /locus_tag="ACHLCY_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP0369783.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16947..17396)
                     /gene="yqcA"
                     /locus_tag="ACHLCY_002551"
     CDS             complement(16947..17396)
                     /gene="yqcA"
                     /locus_tag="ACHLCY_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP0369784.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17414..18196)
                     /gene="truC"
                     /locus_tag="ACHLCY_002552"
     CDS             complement(17414..18196)
                     /gene="truC"
                     /locus_tag="ACHLCY_002552"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP0369785.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18196..18525)
                     /gene="yqcC"
                     /locus_tag="ACHLCY_002553"
     CDS             complement(18196..18525)
                     /gene="yqcC"
                     /locus_tag="ACHLCY_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP0369786.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19147..19692)
                     /gene="syd"
                     /locus_tag="ACHLCY_002554"
     CDS             complement(19147..19692)
                     /gene="syd"
                     /locus_tag="ACHLCY_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP0369787.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19760..20608
                     /gene="queF"
                     /locus_tag="ACHLCY_002555"
     CDS             19760..20608
                     /gene="queF"
                     /locus_tag="ACHLCY_002555"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP0369788.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20720..22084
                     /gene="ppnN"
                     /locus_tag="ACHLCY_002556"
     CDS             20720..22084
                     /gene="ppnN"
                     /locus_tag="ACHLCY_002556"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP0369789.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22641..23930
                     /gene="sdaC"
                     /locus_tag="ACHLCY_002557"
     CDS             22641..23930
                     /gene="sdaC"
                     /locus_tag="ACHLCY_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP0369790.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23988..25355
                     /gene="sdaB"
                     /locus_tag="ACHLCY_002558"
     CDS             23988..25355
                     /gene="sdaB"
                     /locus_tag="ACHLCY_002558"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP0369791.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25467..26222
                     /gene="xni"
                     /locus_tag="ACHLCY_002559"
     CDS             25467..26222
                     /gene="xni"
                     /locus_tag="ACHLCY_002559"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP0369792.1"
                     /translation="MAVHLLIVDTLNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26277..27425)
                     /gene="fucO"
                     /locus_tag="ACHLCY_002560"
     CDS             complement(26277..27425)
                     /gene="fucO"
                     /locus_tag="ACHLCY_002560"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP0369793.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27453..28100)
                     /gene="fucA"
                     /locus_tag="ACHLCY_002561"
     CDS             complement(27453..28100)
                     /gene="fucA"
                     /locus_tag="ACHLCY_002561"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP0369794.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6875 a         7521 c         6743 g         7102 t
ORIGIN      
        1 atcatgtcaa aacattcacc aaaattatat ttattttttc tgtgttagtt tttaatgatg
       61 acgaatctaa aattaccgat gccgccgtca atttatttat ccagatttaa tgagaaattt
      121 ataatgcgct atttcatact aatgttcact ttcgtgtgtt cctttgttgc agcccaacca
      181 actattgtcc cacaattaca gcaacaagtt acggatctta cgagtagctt aaactcacaa
      241 gaaaagaaag aactgacgca caagttagaa tctattttca ataataccca agtgcaaatt
      301 gctgtattaa ttgttcccac aaccaaagac gagacaattg aacaatatgc tacaagagtt
      361 tttgacaatt ggcgtttagg agatgccaaa cgtaatgatg ggatactgat cattgttgcc
      421 tggtcagatc gtactgtccg catccaggta ggttatgggc tggaagaaaa ggtaaccgat
      481 gctctggctg gagatatcat ccgtagcaac atgatacccg catttaaaca acaaaaattt
      541 gctcagggat tagagctagc tataaacgct ttgaataacc aactcacttc acaacaccaa
      601 tatccgacta atccttcaga aagtgaatca gcgtcttcca gtgatcatta ttactttgct
      661 attttttggg tatttgcagt gatgttcttc cctttctggt tttttcatca aggcagtaat
      721 ttttgtcgcg catgtaaaag tggagtctat atttcagcaa tctatctttt agatttattc
      781 ctgttctccg ataaaatttt ttccattgct gtattttcct tcttttttac tttcaccata
      841 tttatggtct ttacctgttt atgtgtactt cagaaaagag catcaggtaa aagttatcat
      901 tcagacaaca gcggttccgg tggaggctca agtggcggcg gatttagcgg cggtggcggt
      961 tcttctggag gtggcggcgc atccggccgc tggtaactca accataaaaa atgccagccc
     1021 ggaggctggc atttttaaat cagataaagt cagtcttatg cctggccttt gatctcttta
     1081 cgaccgttgt acggtgcttt ttcgcccaga gcttcttcga tacgaatcag ctggttgtat
     1141 ttagcaacac ggtcagaacg gctcatagaa ccagttttga tctggcctgc agcagtacca
     1201 acagccaggt cagcgatggt agcgtcttca gtttcgccag aacggtgaga gataactgca
     1261 gtgtagccag catctttcgc catcttgatt gcagccagag tttcggtcag agaaccgatc
     1321 tggttgaatt tgatcaggat ggagttagcg ataccttttt cgataccttc tttcaggatc
     1381 ttggtgttgg ttacgaacag gtcgtcacca accagctgga ttttgtcgcc cagaactttg
     1441 gtctggtatg cgaaaccgtc ccagtcagat tcgtccagac cgtcttcgat agaaacgatc
     1501 gggtactgtt tggtcagttc ttccaggaag tgagtgaatt cttcagaggt gaacgctttg
     1561 ttgccttcgc cagccagaac gtatttaccg tctttgtaga attcagaagc tgcgcagtcc
     1621 atcgccagag tgatgtcttt gcccagttca taaccagcag ctttaacagc ttcagcgata
     1681 acagccagag cttcagcgtt ggaacccagg ttcggcgcat agccaccttc gtcaccaact
     1741 gcagtgttca tgcctttcgc tttcagaact tttgccaggt gatggaaaac ttcagaaccc
     1801 atgcggatgg cttctttcac agttttcgcg ccaaccggct gaatcatgaa ttcctggata
     1861 tcaacgttgt tgtcagcgtg ctcaccaccg ttgatgatgt tcatcatcgg aaccggcata
     1921 gagtatttgc ccggagtacc gttcagttca gcgatgtgct cgtacagcgg cataccttta
     1981 gcagctgcag cagctttggc gttagccaga gatacagcca ggattgcgtt cgcgccgaat
     2041 ttggatttgt tttcggtgcc gtccaggtcg atcatgatct tgtcaatgcc agcctgatct
     2101 ttagcatctt tgccaatcag cgcctgagcg atcgggccgt ttaccgcagc aacagctttg
     2161 gttacgcctt tacccaggaa acgggatttg tcgccatcgc gcagttccag agcttcacgg
     2221 gaaccagtag aagcacctga cggagcagct gccataccga cgaaaccacc ctccagatgt
     2281 acttcggctt caacagtcgg gttaccacgg gagtcgatga tttcacgacc gatgattttt
     2341 acgattttgg acattaggtt ttcctcaagt cactagttaa actgaaactc cagacaaaca
     2401 acgcgtaccc agggtacgcg ttgccgttct aactttttta cttacttcgc ctgacgtttc
     2461 tggaactcgc tggcggcttt cacaaagcct gcaaacagcg ggtgaccatc acgtggagta
     2521 gaagtaaact ccggatggaa ctggcaagcc acgaaccacg ggtgattcgg aacttcgatg
     2581 atctcgacca actgatcatc cccggaacgg cccgcaacgc gcagacctgc atcttcaatc
     2641 tgtttcaaca gcatgttgtt gacttcgtaa cggtgacgat gacgctcaac aattgtcggc
     2701 gcattgtaca gctggcgaac caggctatcg tcaaccaact ggcactgctg tgcgccgaga
     2761 cgcatggtac cgccgagatc gctcttctcg ctacgaactt caacgttgcc gttttcatcg
     2821 cgccactcgg taatcagcgc cacaaccggg tacttacagt ctggcacaaa ttccgtagag
     2881 ttggcgttct ccatgttggc aacatggcga gcgtaatcaa ttaacgccac ctgcataccc
     2941 aggcaaatgc ccagataagg aatattgttc tcacgcgcaa aacgcgcggt cgtaatcatg
     3001 ccttctacgc cacgatagcc gaaaccgcca ggtacgagga ttgcgtccag acctttaagg
     3061 atttcaacgc cgcgcgtttc aacatcttgt gaatcgatca gtttgatgtt gacgctgaca
     3121 cgattcttca gcccaccgtg tttcagtgct tcgatcactg atttataagc atccggcagt
     3181 tcaatgtact tgccgaccat accgatggtg acttcactta ccgggttcgc ttcttcgaag
     3241 ataacctgtt cccattcgga cagattcgct tccgggcagt ttaagctgaa tcgtttacaa
     3301 atataatcgt ccagcccctg agatttcaac aggcccggaa ttttatagat ggaatcgacg
     3361 tctttcagag aaataaccgc tttttccgga acattacaga acaatgcaat cttcgcacgt
     3421 tcgttcgccg gaacagcgcg atctgaacga caaatcagga tgtcaggctg gataccgatg
     3481 gagagcagct cttttacaga gtgctgagtc ggtttggttt tgacttcacc agacgctgcc
     3541 atgtacggca ccagcgtcag gtgcataaac agagtgtgct cacggccaat ttcaacagcc
     3601 atctggcgaa tcgcttcgag gaacggcaag gattcgatat cacctactgt accgccgatt
     3661 tctaccagta ctacgtcatg accttcgcca ccttccagca cgcgctcttt gattgcgtta
     3721 gtgatgtgcg gaataacctg cacggttgcg ccgaggtagt caccgcggcg ttctttacgc
     3781 agaacgtcag agtagatacg acccgtggtg aagttgttgc ggcggctcat tttggtacga
     3841 atgaaacgct cgtagtgccc caggtccagg tcggtttcag cgccgtcttc agtaacgaac
     3901 acttccccgt gttggattgg gctcatagta cctggatcga cgttgatgta cggatccagt
     3961 ttcatgatgg tcacattgag gccacgggct tcaagaatgg ctgcgaggga ggctgcggca
     4021 atgcctttac ccagagagga tacgaccccg ccggtcacaa aaatatagtt cgttgtcatg
     4081 ctgaacctga gaagttaggt tgaaaagacg atggaataac caggacggga aagcagtata
     4141 cccgaacatg acctgtgcca caaactttca ttatccctcc tcttcgccag cgcactattg
     4201 aaatcaggag tgagaaaata gcccctttgg ggtaaatgtt tttgacgcaa atcaagcgct
     4261 tgtcatttaa aaaatgacac aaatggcgct tgaccgcgta attcccttag agatcaattt
     4321 cctgccgttt tacctgttgc cagacttctt ccattgtttc gaggtcaaca cctgtcattt
     4381 ccagtccacg cgcggcaaca atacgctcca cttcgcgaaa acgacgctca aatttttcgt
     4441 tcgctttttg caatgcgatt tccgcttttg tccctaaatg acgagccaga ttgaccgtgg
     4501 caaacagcag gtcccccatt tcctcctcca gtttagcctg gtcgacaaca gcctgccgcg
     4561 cttcgtacat cacctcgtcg atctcttcgt agactttatc gactaccgga ccaagcgtcg
     4621 tccaatcgaa gccaacgttg gcgcaacgtt tctggatttt ttgcgcacgc attaaagccg
     4681 gtaaactacg aggaatatcg tccagcgccg aatgctgcgc tttctgcgcg cgctcttcgg
     4741 ttttgatttg ctcccaacgg gcaagcactt cactactgtt ttcggcagaa ctatcagcaa
     4801 aaacatgcgg atggcgacgc tctaatttat cgctaatagc agcgcaaata tcattaaagt
     4861 caaagcgccc ttcttcctga gccatttgcg cgtaaaacac cacctggaat agcagatcgc
     4921 ccagttcacc gcgaagatcg tcaaaatctt cacgggcgat ggcgtccagc acttcgtagg
     4981 tttcttcaag ggtgtaaggc gcaatggtgg caaatgtctg ctctttatcc cacgggcagc
     5041 cgttttccgg atcgcgcagg cgctgcataa tagtgagcaa acggtcgatt tgattcattg
     5101 aattgtcctg aaaattgcgg gtctgtcagg tggaaacctg tgaccagaat agaagtgagt
     5161 tagtaacact acccaatcag tacgttaatt ttggctttaa tgagttgtaa ttcctctggg
     5221 gcaactgttc ctttcttcgt tgctcctctt gcccgccagg cgatactttt tacctgatca
     5281 gctaacgcta cgccatcacg ttcctgaccg gataaaacaa cttcgaacgg atatcctttt
     5341 gattgcgttg tataaggaac acacagacac atacctgttt tgttgttgta catgaaagga
     5401 ctcaggacaa cagctggacg atgtccagct tgctcgctac cttttgtcgg gtcaaaatca
     5461 acccaaatca gatcgcccat atcgggtacg tatcggctta ccattaccag acttccttat
     5521 ctttcggctc tccccagtcg atattctcgt ggaggttttc cggcgtgatg tcgttaacca
     5581 gttcagcaag cgtaaatacg ggctctttac gcactggctc aataattaat ttgccatcca
     5641 ccaggtcaat cttcacttca tcatcaatat tcagattgag cgcctgcatt aacgtagccg
     5701 ggatccgcac cgccggtgaa tttccccaac gctttacgct actgtggatc ataacccttt
     5761 cctcaaaccg ctatcatatg tagatacagt atatatcaat ctacattgta gatacgagca
     5821 aatttcggcc taactcccgt gcaaccgacg cgcgtcgata acatccggca cctggttgag
     5881 tttacccagc acgcgcccca gcacttgcag gttgtaaatc tcaatggtca tgtcgatggt
     5941 cgccagttgc tgtttggtgt cgctacggct ggcaacgcca agcacgttca ccttctcgtt
     6001 ggcgagaatg gtcgtgatat cacgtaacaa cccactacga tcattagcca ccacgcggac
     6061 caccagcgaa tatccggcgg agtagctctc accccatacc gcgtcaacaa tgcgttctgg
     6121 cgcatgggag cgcagttccg ccagttgttc gcaatcggcg cggtgtactg aaataccgcg
     6181 cccctgggta atgaagccga caatctcatc tccaggaatc ggctggcagc agcgcgcgat
     6241 gtggtgcatc aggttgccaa caccttcgac caccacgcga ccgttatctt tactgcggtt
     6301 ttgcggcgtg tagctttttt gctgaagttg cttcagcgcg gcggcgtcct gctcttcggc
     6361 actcggctta ttaaattgcg attgcaggaa gttcaccatc tgattgagac ggatatcccc
     6421 gccaccaatc gccgccagca actcgtcgac atcattaaag ttgtaacgcg gcagcagatg
     6481 tttttctgct tctttcaggc tgatccccag atgttccagc tcgtcgtcaa ggatttgccg
     6541 cccagccaga atgtttttgt cacggtcctg tttacggaac caggcgtgaa ttttcgaacg
     6601 cccacggctg gttgtgacgt aaccgaggtt tgggtttaac cagtcacggc tggggttcgg
     6661 ctgtttctgg gtgataattt caatctggtc gcccatctgc agctggtagg tgaacggcac
     6721 aatgcgcccg ccaatttttg ccccgatgca gcggtgtccg acatcactgt ggatgtggta
     6781 agcgaagtcc agcggcgttg atcccgcagg caaatcaacg acatcacctt tcggcgtaaa
     6841 gacatacacc cggtcgtcaa agacctggct gcgtacttcg tcgagcattt cgccggaatc
     6901 agccatctct tcctgccacg caatcagttt acgcagccag gcaatccggt cttcatgtcc
     6961 cgaacgtgcg ccgccagctg ccgcgccctc tttatatttc cagtgcgcag caacacccaa
     7021 ctctgcatct tcatgcatct gtttggtgcg gatttggatc tcaacggttt tgccacctgg
     7081 ccccagaacc acggtatgaa tagactgata accgtttggt ttcgggttag cgacataatc
     7141 gtcaaactca tccggcaggt ggcgatagtg agtgtgcact atccccagtg cggcataaca
     7201 atcctgtaaa cgctcggcga caatacgtac cgcacgcaca tcaaacagct catcgaaggc
     7261 gaggttcttt ttctgcattt tgcgccagat actgtagatg tgtttcggac gaccatacac
     7321 ttcagcttta acgccttcag ctttcatctc agcgcgcaga tggccgacaa actcttcgat
     7381 atagtgttcg cggtcgagac ggcgttcatg cagcagtttt gcgatgcgtt tgtattcggt
     7441 cgggtggaga taacggaagc agtaatcttc cagctcccat ttcagttgcc caatcccaag
     7501 acggttcgcc aacggcgcgt agatattggt gcactctttt gccgccagta cgcgttcatc
     7561 ttccggcgca tcttttactt cgcgcagatg agcaatacgc tccgccagtt tgatgaccac
     7621 gcagcgaaaa tcatcgacca tcgccaataa catccggcga acgttatcga cctgttcgga
     7681 ggaaacagaa tcagtgtgcg tcgctttcag ctggcggatc gccgccatat cacgcacgcc
     7741 gtgaataagg ttaacgaccg acttaccgac gctctcacgc agcacatctt cgctgactac
     7801 gttggcatca gccagaggga acagcagcgc cgcccgcagc gtgtcaatgt ccatacttaa
     7861 tgtcgagagg atctccacca tctcaacacc acgccacaat aacagactgg catccggatg
     7921 cccctgcgtc tgttgcagac aatacgccca ggtttcggct aagcactcac acgacttctg
     7981 gctggtaata cccagacttg cgatccattt ttccggatca aattcaccag ccttattgat
     8041 atgtgcactt cttaccgcaa ccattgtcct ctcctttagg gaccagacct gccgaaatcg
     8101 gcaaatcgta actatttaac gcgcgagaaa agtaccatcg attccagatg tcccgtgtgt
     8161 gggaacatat ccagcatcgc cagtcgcgca atggtatatc ctgcttttaa taacgcttcg
     8221 ctatcccgag ccagcgttgc agggttacag gatacataaa ctatacgaat aggttccagt
     8281 tttataattt gctgcataac acctgcggca cctgctcgcg ccgggtccag caacacttta
     8341 tcgaagccgt ttttcgccca cggctgcttt gtgacatctt cttcaagatt ttcgtgataa
     8401 aacgtcacat tctgtaaacc attaagacgc gcattctgct ggcctttttc caccagcgcc
     8461 ggaacacctt ctacaccgac cacactggct gcttgtttcg ccaatggcag tgtaaagttg
     8521 cccataccgc agaacagatc cagtacgcga tcttcaggtt gcacatccag ccattccaac
     8581 gcacgcgcta ccatttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     8641 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     8701 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     8761 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     8821 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     8881 ccctgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     8941 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     9001 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     9061 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     9121 ctacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggtg
     9181 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     9241 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     9301 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     9361 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     9421 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     9481 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     9541 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     9601 ggtgccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     9661 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     9721 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     9781 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9841 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9901 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     9961 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
    10021 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
    10081 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
    10141 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
    10201 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
    10261 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
    10321 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
    10381 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
    10441 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
    10501 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
    10561 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
    10621 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
    10681 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
    10741 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
    10801 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10861 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
    10921 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10981 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    11041 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    11101 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    11161 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    11221 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    11281 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    11341 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    11401 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    11461 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    11521 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    11581 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    11641 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    11701 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    11761 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    11821 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtgatg
    11881 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11941 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    12001 gaggaacagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    12061 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    12121 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    12181 gagatggata atgtagcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    12241 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    12301 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    12361 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    12421 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    12481 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    12541 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    12601 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    12661 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    12721 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    12781 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12841 catcgtccag aaatgcggat ccgccagcgg gatatcaacg gattgcaggg agagcgtatg
    12901 gcccatctgc cgccagtcgg tggcgatcat attggttgcg gtcggcaggc cagttgcgcg
    12961 acggaactca gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    13021 tgcataagcc agcgaccctt tcaggtattt accaatttta atcgcttcgt tcagcgacca
    13081 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    13141 agactcggcc tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    13201 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    13261 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagcggtgt
    13321 ggctttgcga tcaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    13381 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    13441 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    13501 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    13561 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    13621 ctcgccgccg ggaatttccc ctacgccagt gtgaccagaa ttatctttga taatcacaat
    13681 attacgcgta aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    13741 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    13801 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13861 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    13921 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13981 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    14041 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    14101 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    14161 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    14221 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    14281 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccatc
    14341 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    14401 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    14461 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgccc gaacagtgtc gatttcttgc
    14521 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    14581 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    14641 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    14701 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    14761 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    14821 gccacggcat taacgcgcaa ttcaaaagtc caggcacttt tgccgaaggt atcaaaatcg
    14881 gctacctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    14941 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    15001 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    15061 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    15121 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    15181 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    15241 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    15301 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    15361 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    15421 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    15481 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    15541 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataatcccac ccagcacacc
    15601 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    15661 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    15721 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    15781 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    15841 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    15901 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    15961 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    16021 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    16081 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    16141 gaccaggaag cgcagcgtaa acagggcaac gataatgccg aatccactaa agatatcgac
    16201 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    16261 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    16321 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    16381 cgaaccagca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    16441 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    16501 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    16561 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    16621 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    16681 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    16741 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    16801 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    16861 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16921 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    16981 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag tatttcgcca
    17041 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    17101 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    17161 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    17221 gtcgtggacg taaccaccag aacatactta tcctgatagg gcagccagtc gcttaattca
    17281 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    17341 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    17401 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    17461 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    17521 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    17581 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    17641 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    17701 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    17761 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    17821 ctgctggctg cgagccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    17881 ccaccagtgg ataatccaga actgcttctt ccatcaacca gccgcgcaca atcgcatggt
    17941 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    18001 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagcg gtaaaaacgt
    18061 gctggccaat ctgatcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    18121 agctgcggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    18181 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccagctc
    18241 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    18301 aacggcaaaa gcgcccggta acggctgttt gttatctaac aaatcgtgca ttcgcgggat
    18361 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    18421 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    18481 ttcaagcgcc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacctcaata
    18541 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagcgct
    18601 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    18661 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    18721 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    18781 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    18841 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    18901 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    18961 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    19021 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    19081 cttatttcct ggcgatctcc tacagagtct gtaagagatc tctcacaacc agatggggta
    19141 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    19201 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    19261 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    19321 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    19381 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    19441 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    19501 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    19561 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    19621 atgctcttca tgccacgcat cgcagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    19681 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    19741 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    19801 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc aacctactgc aaggcgttcc
    19861 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    19921 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggttgc
    19981 cgtcggtcac gttgaacttg attacaccag cgcaaatctg attgagtcga agagttttaa
    20041 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    20101 gctggagcgc gacttaagca cttgcgccca gggtaaggtt agcgtggcgt tatatcgtct
    20161 tgatgaactg gaaggccagc cgataggtca tttcaatggc acctgcattg atgaccagga
    20221 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca ccagcggtga
    20281 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    20341 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    20401 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    20461 acgcatcttt aatgacctgt tacgcttctg ccagccagag aaattaagcg tttacgcacg
    20521 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    20581 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    20641 cagaaggttg tgaaaggtca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    20701 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    20761 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    20821 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    20881 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    20941 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgctttgca
    21001 ggccaacttg ttcgcggttc tgcgagacat tctcttcgtt tacgggcaaa tccataatac
    21061 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    21121 catcttgcgt aacgctcgcg cgctgcatgt gggtgaagcg ccaaatatgg tggtctgctg
    21181 gggcggtcac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg gaaaccagct
    21241 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    21301 gaaaggtgct gcggtcggac acgcgcagca gcgttacaaa gacagtcgtt ttattggtat
    21361 gaccgagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    21421 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gtatcattat
    21481 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    21541 cccggccaac aaagatcagg ttttaccatt gatcctcacc ggcccgaaag agagcgccga
    21601 ctacttccgc gtactggacg agtttgtcgt acatacgctg ggcgaaaacg cgcgccgcca
    21661 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    21721 gctggtgaaa gaaaatcgcc gcgacactgg tgacgcttac agctttaact ggtcaatgcg
    21781 cattgcacca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    21841 gctttacccg gatcaacctg ttgaagtgct ggctgccgac ctgcgccgtg cgttctccgg
    21901 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    21961 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    22021 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    22081 gtaatctccc ctcccccggt gacgtttgtt gccgggtttt ctcgtttttg gtcacttact
    22141 catcaactca tttcatttgt tatatgaatg tttcttacca ccctcacgcg acaaatatca
    22201 tcacagttaa tatgtcatac aatttatatt gcaacgcaaa cgtttaccta ttttcataaa
    22261 ccgtatttta tcgctatgaa aaagaaatta tcgccatgat taactaaaag tattgatttt
    22321 ttcagttcaa cctacatata ttgcgcgccc cggaagaagt cagatgtcgt ttaatgggca
    22381 aatattgccc ttaaattctc ttttactttt gatttacaga gtaaagcgtt gggataatct
    22441 atcttccaag tagattattg tatttgggat caagatcact gatagataca taacttgtgt
    22501 gtatctttcc gccctcaaat tattacggcg gtaaatgatt aagccatcgc cgatagacag
    22561 atttcatttt tacggtcagg caccttcccg ggctgaactg gctaaaagct gaattatttg
    22621 cattcctcca ggagaaatag atggaaacga ctcaaaccag cacgattgcg tcgaaagact
    22681 ctcgtagtgc ctggcgcaag acagacacca tgtggatgct gggcctttac ggcacggcaa
    22741 tcggcgcggg cgtgctgttc ctgccaatca acgccggtgt tggcggtatg atcccgctga
    22801 tcatcatggc tatccttgcg ttcccgatga cgttttttgc tcaccgcggc ctgactcgct
    22861 tcgtactgtc tggtaaaaac ccgggcgaag acatcaccga ggttgtagaa gaacactttg
    22921 gtattggcgc aggtaaactg attaccctgc tctacttctt cgctatctac ccgatcctgc
    22981 tggtttatag cgtggcaatc accaatacag ttgaaagctt catgtctcac cagctgggta
    23041 tgacgccacc gccgcgtgcg attctgtcgc tgatcctgat cgtgggtatg atgaccatcg
    23101 ttcgctttgg tgagcagatg atcgttaaag cgatgagtat tctggtattc ccgtttgttg
    23161 gcgtactgat gctgctggct ctgtacctga tcccgcagtg gaacggcgca gcactggaaa
    23221 cgctgtctct ggacactgca tctgcaaccg gaaacggtct gtggatgacc ctgtggctgg
    23281 caattccggt aatggtgttc tcgttcaacc actctccgat catctcttct ttcgccgttg
    23341 cgaagcgtga agagtacggc gatatggcag aacagaaatg ctcgaagatc ctggcattcg
    23401 cacacatcat gatggtgctg accgtaatgt tcttcgtctt cagctgcgta ctgagcctga
    23461 ctccggcaga cctggctgcg gctaaagagc agaacatctc gattctgtct tacctggcta
    23521 accactttaa cgcaccggtt atcgcgtgga tggctccgat tatcgcgatt atcgctatca
    23581 ccaaatcctt cctcggccac tacctgggcg cacgtgaagg cttcaacggt atggtgatta
    23641 aatctctgcg tggtaaaggt aagtctatcg aaatcaacaa gctgaaccgt atcactgcgc
    23701 tgttcatgct ggtaacgacc tggattgttg ccaccctgaa cccgagcatc ctgggtatga
    23761 ttgaaaccct gggcggccca atcatcgcga tgatcctgtt cctgatgccg atgtacgcaa
    23821 ttcagaaagt accggcaatg cgtaagtaca gcggtcacat cagcaacgta ttcgttgtcg
    23881 tgatgggtct gattgcaatc tccgcaatct tctactctct gttcagctaa gtcctttcgc
    23941 gccgctttcg ggcggcgctt cctccgtttt aacgcgatgt atttcctatg attagcgtat
    24001 tcgatatttt caaaatcggc attggccctt ccagttctca taccgttgga ccaatgaaag
    24061 cgggtaaaca atttaccgac gatctgattg cccgtaacct gcttaaagac gtgacccgcg
    24121 tggtggttga cgtgtacggc tcgctctctc tgactggtaa aggccaccac actgatatcg
    24181 ccattattat gggcctggcg ggtaacctgc cggataccgt ggatatcgat tccatccctg
    24241 gttttattca ggatgtgaat actcatggtc gcctgatgct ggcaaacggt cagcatgaag
    24301 tggagttccc ggttgatcag tgcatgaact tccatgcaga caacctttct ctgcatgaaa
    24361 acggtatgcg cattaccgcg ctggcgggcg ataaagtcgt ttacagccag acttactact
    24421 ctattggcgg tggctttatc gttgatgaag agcattttgg ccagcagaat agcgcaccgg
    24481 ttgaagttcc ttatccgtac agttcagcag ccgatctgca aaaacattgt caggaaaccg
    24541 ggctgtcact ctctggcctg atgatgaaaa atgagctggc gctgcacagc aaagaagagc
    24601 tggaacagca cctggcgaac gtatgggaag tcatgcgtgg cggtattgag cgcggtattt
    24661 ccaccgaagg cgtgttgcct ggcaaactgc gcgttccacg ccgtgctgcg gcactacgcc
    24721 ggatgctggt cagccaggat aaaaccacca ctgacccgat ggcggttgtt gactggatca
    24781 acatgtttgc attggcagtg aacgaagaga acgctgctgg cggacgcgtg gtgactgcgc
    24841 cgactaacgg tgcgtgcggg attatcccgg cagtgctggc gtactacgac aagtttatcc
    24901 gcgaagtgaa cgctaactca ctggctcgtt acctgctggt agccagtgcc attggttctc
    24961 tttataagat gaacgcgtca atttctggtg ctgaagtcgg ctgccagggt gaagttggtg
    25021 tggcgtgctc aatggcggcg gctggtctgg cagaactgtt aggtgcaagc ccggcgcagg
    25081 tgtgcatcgc ggcggaaatc gccatggagc acaacctcgg tctgacgtgt gacccggtcg
    25141 ccggacaggt ccaggtgcca tgcatcgagc gtaacgccat tgcggcagta aaagcggtga
    25201 acgccgcacg tatggcgcta cgccgtacca gcgagccgcg cgtctgcctc gataaagtta
    25261 tcgaaaccat gtacgaaaca ggtaaagata tgaacgccaa gtaccgcgaa acctctcgcg
    25321 gcggcctggc aatgaagatc gttgcctgcg attaatcgct ctccagaggc ctcgttttgc
    25381 gaggcctctt cccgatttct catccagccg tagcctgttc cggcatcgaa tgttaccctt
    25441 atcgcctgat ctttaagggg gttatcgtgg ctgttcattt gcttattgtc gatacactga
    25501 atcttattcg tcgcattcat gccgttcagg gatcgccctg tgtcgaaacc tgccagcatg
    25561 cgctcgatca gctcattatg cacagccagc caacccacgc ggtcgccgtt tttgatgatg
    25621 aaaaccgcag tagcggctgg cgtcatcagc gtttaccaga ttacaaagcg ggtcgaccgc
    25681 caatgccgga agagttgcac gacgagatgc ctgcattacg cgccgccttt gagcaacgcg
    25741 gcgtcccgtg ctggtcaacc agcggcaacg aagccgatga cttagccgcc acgctggcgg
    25801 tcaaagtgac acaggccggg catcaggcaa cgattgtttc gacagataaa ggctactgtc
    25861 agttactttc accgacatta cgtattcgtg attacttcca gaaacgttgg ctggatgcgc
    25921 catttatcga taaagaattt ggcgttcaac cgcagcagtt gcccgattac tggggacttg
    25981 cggggatcag cagttcaaag gtaccgggtg ttgcgggaat cggaccaaaa agcgccacgc
    26041 agctgctggt cgagtttcag agtctggaag ggatatatga gaatctggat gcggttgccg
    26101 aaaagtggcg caaaaaatta gaaacccata aagagatggc gtttctgtgc cgcgatattg
    26161 cccgcttaca aaccgatttg catatcgacg gcaatttaca gcaattgcgg ttggtacggt
    26221 aacggcgagc cggatacgcc gcaaacgtcg tatccggcat tatcacatca gcgcatttac
    26281 caggcggtat ggtaaagctc tacaatatcc tcaagcgttg cttcacgcgg gttgccaccg
    26341 gtacaaacat catccagtgc cgcctgcgcc agtgccggaa tgtcttcctt gcgtacccca
    26401 acatcacgca aatgtggcgg aataccgaca tcacggttga gagcaaacac cgcttcaaca
    26461 gcggcattac gcgcctcttc caggctcata ccttccactt tcacgcccat aacgcgcgcg
    26521 atatcgcggt acttctcacc ggtaaagtca gcgttatagc gcatgacatg cggtagcagg
    26581 atggcgtttg caacaccgtg tggagtgtta taaaacgcgc ccagtggatg cgccatacca
    26641 tgcaccaacc ctaacccaac attcgagaag cccatacccg caacatactg cccgagcgcc
    26701 atttcttctc cggcatcctt atcaccagca accgatcctc gcagcgcccc agcaatgatt
    26761 tcaatcgctt taatgtgcag tgcatcggtt agcgcccacg cgccacgggt aatatacccc
    26821 tcaatagcat gagtgagcgc atcgacaccc gtcgcagctt tcagcgctgg aggcatacca
    26881 tccatcatgt cagcgtcaat aaacgccacc tgcgggatat catgcggatc aacgcaaaca
    26941 aacttgcgcc gtttttcttc gtcagtgatc acgtagttaa tggtcacttc tgccgcagtg
    27001 cctgctgtgg tggggattgc cagaatcggt acactgggtt tattggtcgg ggaaagtcct
    27061 tccaggctac gcacatcggc aaactccggg ttgttgctga taatgccaat cgctttacaa
    27121 gtatcctgtg gagaaccacc accaatagcg atcaggtaat ccgcgccgct attctggaat
    27181 acaccgagcc cttctttgac gacagtaatt gttgggttgg gcactacgcc gtcgtaaatc
    27241 gcccatgcca gccctgcagc atccatctta tcggtcactt tcgccaccac gccgcattgc
    27301 accagcgttt tatcggtgac gatcagcgcc ttctgataac cacggcgttt cacctcatcg
    27361 gttaaagccc caacagcacc ccgaccaaac catgccgttt cgttcagaat cattctgtta
    27421 gccatcatcc ttctccttgt tgctttacga aattactctt caattcgtaa cccataggtt
    27481 ttgaatttct ccagcactac ggcaatctct tcatcgctca gcactggcac cgggtccgta
    27541 atcgccaggg tcgtcaggta aagttgcgcc agcacttcaa cttcatgcgc cagccataac
    27601 gctttttcca gattcacctc acaagcgata agcccatgat gttgtaataa agttgcctta
    27661 cgatttttga gagcctgcgc aacatgttca gaaagttcac gtgttccaaa ggtcgcataa
    27721 ggcgcgcaag gaatagaatt accgccagcc gccgcaatca tgtagtgaat agcggggatc
    27781 gatcggttaa gaatggaaac tgccgtgcaa tgaacggcat gattgtgaac aaccgcgttg
    27841 gcatccggtc tgctttgata ggccgccata tggaaacgcc attcgcttga ggggagcttt
    27901 ccttcctcat gtttaccgtt gccatcaata aagacaatat gcgactccgt cagtttttca
    27961 tatggaatgc ctgtaggcgt aatcagcatc ccatcctgat aacgtacact gacgttcccc
    28021 gctgtcccct ggttcagtcc caggcgggtc atttccaggc aagtgtcaat aatctgacga
    28081 gcaagtttat ttcgttccat tagctacctc tctctgattc aaaacagggc aataatgttg
    28141 ttcctttcac actattgaat tagccgttta attacccacc atcttcttcc tgattaacca
    28201 gaaagaaatt cacaagctta tattttgtga cctggttcaa c
//
LOCUS       DAZGTR010000055        27291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGTR010000055 DAZGTR010000000
VERSION     DAZGTR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.055"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(150..2141)
                     /gene="tkt"
                     /locus_tag="ACHLCY_002562"
     CDS             complement(150..2141)
                     /gene="tkt"
                     /locus_tag="ACHLCY_002562"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0369795.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2419..3177
                     /gene="loiP"
                     /locus_tag="ACHLCY_002563"
                     /gene_synonym="yggG"
     CDS             2419..3177
                     /gene="loiP"
                     /locus_tag="ACHLCY_002563"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP0369796.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3233..3976)
                     /locus_tag="ACHLCY_002564"
     CDS             complement(3233..3976)
                     /locus_tag="ACHLCY_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0369797.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3963..5072)
                     /gene="dgaE"
                     /locus_tag="ACHLCY_002565"
     CDS             complement(3963..5072)
                     /gene="dgaE"
                     /locus_tag="ACHLCY_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFP0369798.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5076..5936)
                     /locus_tag="ACHLCY_002566"
     CDS             complement(5076..5936)
                     /locus_tag="ACHLCY_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP0369799.1"
                     /translation="MMSDDVMQHELVERARESGALTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEAINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(5933..6682)
                     /locus_tag="ACHLCY_002567"
     CDS             complement(5933..6682)
                     /locus_tag="ACHLCY_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP0369800.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6708..7193)
                     /locus_tag="ACHLCY_002568"
     CDS             complement(6708..7193)
                     /locus_tag="ACHLCY_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP0369801.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7204..7632)
                     /locus_tag="ACHLCY_002569"
     CDS             complement(7204..7632)
                     /locus_tag="ACHLCY_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0369802.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7751..10549)
                     /gene="dagR"
                     /locus_tag="ACHLCY_002570"
     CDS             complement(7751..10549)
                     /gene="dagR"
                     /locus_tag="ACHLCY_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFP0369803.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10808..11728)
                     /gene="speB"
                     /locus_tag="ACHLCY_002571"
     CDS             complement(10808..11728)
                     /gene="speB"
                     /locus_tag="ACHLCY_002571"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP0369804.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11864..12595)
                     /locus_tag="ACHLCY_002572"
     CDS             complement(11864..12595)
                     /locus_tag="ACHLCY_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369805.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGQAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(12741..14717)
                     /gene="speA"
                     /locus_tag="ACHLCY_002573"
     CDS             complement(12741..14717)
                     /gene="speA"
                     /locus_tag="ACHLCY_002573"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP0369806.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQNMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14726..14857)
                     /gene="yqgB"
                     /locus_tag="ACHLCY_002574"
     CDS             complement(14726..14857)
                     /gene="yqgB"
                     /locus_tag="ACHLCY_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP0369807.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(14951..15094)
                     /gene="yqgG"
                     /locus_tag="ACHLCY_002575"
     CDS             complement(14951..15094)
                     /gene="yqgG"
                     /locus_tag="ACHLCY_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP0369808.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(15205..15456)
                     /gene="yqgD"
                     /locus_tag="ACHLCY_002576"
     CDS             complement(15205..15456)
                     /gene="yqgD"
                     /locus_tag="ACHLCY_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP0369809.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            15512..16666
                     /gene="metK"
                     /locus_tag="ACHLCY_002577"
     CDS             15512..16666
                     /gene="metK"
                     /locus_tag="ACHLCY_002577"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP0369810.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            17102..18496
                     /gene="galP"
                     /locus_tag="ACHLCY_002578"
     CDS             17102..18496
                     /gene="galP"
                     /locus_tag="ACHLCY_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP0369811.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            18573..19070
                     /gene="yggI"
                     /locus_tag="ACHLCY_002579"
     CDS             18573..19070
                     /gene="yggI"
                     /locus_tag="ACHLCY_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP0369812.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            19165..19872
                     /gene="endA"
                     /locus_tag="ACHLCY_002580"
     CDS             19165..19872
                     /gene="endA"
                     /locus_tag="ACHLCY_002580"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP0369813.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            19952..20683
                     /gene="rsmE"
                     /locus_tag="ACHLCY_002581"
     CDS             19952..20683
                     /gene="rsmE"
                     /locus_tag="ACHLCY_002581"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP0369814.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            20696..21646
                     /gene="gshB"
                     /locus_tag="ACHLCY_002582"
     CDS             20696..21646
                     /gene="gshB"
                     /locus_tag="ACHLCY_002582"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP0369815.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            21755..22318
                     /gene="yqgE"
                     /locus_tag="ACHLCY_002583"
     CDS             21755..22318
                     /gene="yqgE"
                     /locus_tag="ACHLCY_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP0369816.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            22318..22734
                     /gene="ruvX"
                     /locus_tag="ACHLCY_002584"
     CDS             22318..22734
                     /gene="ruvX"
                     /locus_tag="ACHLCY_002584"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP0369817.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(22910..23890)
                     /gene="yggR"
                     /locus_tag="ACHLCY_002585"
     CDS             complement(22910..23890)
                     /gene="yggR"
                     /locus_tag="ACHLCY_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP0369818.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWSARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            23908..24612
                     /gene="yggS"
                     /locus_tag="ACHLCY_002586"
     CDS             23908..24612
                     /gene="yggS"
                     /locus_tag="ACHLCY_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP0369819.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            24630..25196
                     /gene="yggT"
                     /locus_tag="ACHLCY_002587"
     CDS             24630..25196
                     /gene="yggT"
                     /locus_tag="ACHLCY_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP0369820.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            25193..25483
                     /gene="yggU"
                     /locus_tag="ACHLCY_002588"
     CDS             25193..25483
                     /gene="yggU"
                     /locus_tag="ACHLCY_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP0369821.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            25491..26084
                     /gene="rdgB"
                     /locus_tag="ACHLCY_002589"
     CDS             25491..26084
                     /gene="rdgB"
                     /locus_tag="ACHLCY_002589"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP0369822.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            26077..27213
                     /gene="hemW"
                     /locus_tag="ACHLCY_002590"
     CDS             26077..27213
                     /gene="hemW"
                     /locus_tag="ACHLCY_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP0369823.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKSSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         6579 a         7410 c         6952 g         6350 t
ORIGIN      
        1 gcatatcgtg atgcatatta cataattgat atttattgca tataaatatt tgtgatctac
       61 aacacgcttt atctattgct tgtccgcaaa cggacatatc aaggtaataa aaaaggtcgc
      121 cgaagcgacc tttttacccg aaatgctaat tacagcagtt cttttgcttt cgcaacaacg
      181 ttatcaacag tgaagccgaa ctcttcaaac agcagctctg ccggagcaga ttcaccgaag
      241 gtggtcatac cgacgatagc accgttcagg ccaacatact tgtaccagta gtcagcaata
      301 cccgcttcta cagcaacgcg tgcagtaacc gctttcggca gtacggattc acggtaagca
      361 gcatcctgct tgtcaaatgc gtcggtagac ggcatggaca ccacgcgcgc tttcacgcct
      421 tcggcagtca gtttttcgta ggcagcaaca gccagttcaa cttctgaacc ggtagcgatg
      481 aaaatcagtt ccggctgacc ggcgcagtct ttcagcacat aaccaccgcg cgcgatgttt
      541 gccagttgct cttcagttcg ttcctgctgc gccaggttct gacgggagag gatcagtgcg
      601 gtcgggccgt cctgacgctc aacaccgtat ttccacgcga ccgcggattc aacctggtca
      661 cacggacgcc atgtagacat gttcggggtt acgcgcagag aagcgacctg ctctaccggc
      721 tggtgagtcg ggccatcttc gcccagaccg atggagtcgt gggtgtaaac catcacctga
      781 cgctgtttca tcagcgcagc catacgtacg gcgttacgtg cgtattccac gaacatcagg
      841 aaggtggagg tgtacggcag gaaaccaccg tgcagggaga taccgttagc aatcgcggtc
      901 ataccgaact cgcgaacacc gtagtggatg tagttacccg cagcatcttc gttgattgct
      961 ttagaaccag accacagggt caggttagac ggtgccaggt cagcagaacc gccgaggaat
     1021 tccggcaaca gcggaccgaa agcttcgata gcattctgag acgctttacg gctggcgatt
     1081 ttcgccgggt tagcctgcag tttagcaatg aactcttttg ctttcgcgtc gaagtcagac
     1141 ggcatttcgc ctttcatacg gcgggtaaat tcagcggctt cctgcggata agctttcgcg
     1201 taagcagcga atttctcgtt ccatgcggat tctttcgcct ggcctgcttc tttcgcatcc
     1261 cactgagcat agatttcaga cgggatttcg aacggtgcat atttccagcc cagctgttcg
     1321 cgggtcaggg caatttcagc gtcgcccagc ggcgcaccgt gggagtcgtg agtaccggct
     1381 ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg tttgtcagtc
     1441 actgcgcgcg cttcttctac tgcgcgtttg atggatgccg cgtcatgacc gtcgatgtcg
     1501 cgaataacgt gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt gaaccagcct
     1561 tcaacgtgac catcaataga gataccgttg tcatcgtaga acgcaatcag tttacccagc
     1621 ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat gcagccgtcg
     1681 cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt aaactgcgcc
     1741 gccagcgttt tttctgcaat cgccatacct actgcgttgg caataccctg ccccagcgga
     1801 ccggtggtgg tttccacacc agcggtgtaa cccacttccg ggtggcccgg agttttagag
     1861 tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt gaggtgcagc
     1921 aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc acggtcagcc
     1981 caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc ggcaatgtca
     2041 gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc gtccatgctc
     2101 agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc agatcggatg
     2161 atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc cggaaaattt
     2221 gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg ccgcttcaga
     2281 aatctctgga tcatgctcgc atgttgcgca atctactcgc ccgtccgctg cgcttttcct
     2341 tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat aatcctgacc
     2401 ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag cgtggcaacg
     2461 gtactgactg gttgccagaa tatggactcc aacggactgc tctcatcagg agcggaagct
     2521 tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca ggcatgtcag
     2581 gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa acgtctgaca
     2641 actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta caaagtgtat
     2701 atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt ctatagcggg
     2761 ctgatggata tgatgacgga taacgaagtc gaagcggtga tcggtcacga aatggggcac
     2821 gtggcgttag gccatgtgaa aaaaggaatg caggtggcac ttggtacaaa tgccgtgcga
     2881 gtagctgcgg cctctgcggg cgggattgtc ggaagtttat ctcaatcaca acttggtgat
     2941 ctgggcgaga aattagtcaa ttcgcaattc tcccagcgcc aggaagcaga agccgatgat
     3001 tattcttacg atcttctgcg ccaacgcggc atcagcccgg caggtcttgc caccagcttt
     3061 gaaaaactgg caaaactgga agaaggtcgc caaagctcaa tgtttgacga ccatcctgca
     3121 tccgccgaac gcgcccagca tattcgcgat cgtatgagcg cggatggggt taagtaagtc
     3181 agacataccg atctacaaca taattgtagg tcggtaacgc ctgaatcccc gatcagagta
     3241 gtgatttcac tttatttagc aaaacggcta catcatccgg tcgggtatat cccgtatcag
     3301 ggtctataat cgagctgtaa acatgtggca taatgcgcgg cacccccgcc tccagacaag
     3361 ttttcagaat cacactgaag ttatccaaat caatcccgcc agtcggctca atcagcgtca
     3421 taccattgcg cgccgccgtg gtcgccagcg cgtatagctc cggaagcgac ctctcgccgc
     3481 ccatcggaaa gaacttcgcc gcgtgtgcgc ccatatctag catcattcgc accgccgtat
     3541 cgcaggagac gcgggcaggc gtaccctggc tgctgctaac gccggtggaa atcagcactt
     3601 cgcccggcgt ccccgtcggg ctaaccagcg cgttgacatg ggtctgctca ccgcccgtag
     3661 ccgcaagcgc gcctgccgcg aaaccacaac cggtaaaggt ctggttaacg tgtgctggat
     3721 gaatctgcgc cgcaatcatc gcagctttgt aatactgcgc cgggtcaccc gcccccagac
     3781 ctactgaaat ggacggtatt tccgccatcc aacgtttaac ttcctggatc ccctcatcca
     3841 ccgaggagaa ctgcgcggaa agcacgccaa tcactgcgtg gccttcggcg gcgtcaaaga
     3901 tttcacgggc attggcgata tctttcgcca gcacattaat tgccacgcga ttacgataga
     3961 aattaatctt ttgcatgatc tgcaatctcc ttcagtcgcg ccacgataag tgccatttcc
     4021 ccttccgcca ccgtacgcgg atcaaggcta aaaacgccct ggtggaggtt ataccgccgt
     4081 gcataaatcg cgatatcgcc accacgcagt tgcgcttcga cgacgcgagc gtcaactccc
     4141 aattcctgcg catttactcg aatacgaata cgccagattg cacgcccggc ttcatcctgc
     4201 tcaatatcag ccgttaaccc gtgaatcgca gaaatcgctt caaccactgg ctggagctgt
     4261 tccgcggtga tgactgcctg cccctggtga taattctcca gcgcgtaaac cagcccaacc
     4321 atgttctcct tgccgatttt catcgccctg gcaatgccgt gatgctgcgc tttgcaggct
     4381 gcaatccaga tttttttacc agtgataaac ccggaggtcg gcgcgttaaa ggcttttgcg
     4441 ccgctgtaga ccaccatatc cgctccgctc accacccagg cacgtaaatc ctcttcggcg
     4501 gcggcatcga caatcagcgg caggttgtac atctgcgcga catgtgcgaa gtcctcgatg
     4561 ttcaacattc ccttctgcac gcagtgatgc gacttcacat acagcagcgc ggcggtcgcg
     4621 tcggtaatgg cactctccag ctgccagaga gccgccaggt tacttgaacc cacctccaca
     4681 acgcgcccgc cacctaagcg gatcgcgctg aggatcgatg caccgtaatc gacgttatgg
     4741 ccgcgcagca tcaccacttc attggccatg ccggagctat ccggcatgag cgccacccgg
     4801 gccttgtcgc cgcgtgtaat ggcggcggcg acagcgatgg cgatccctgc tgaggcacag
     4861 gaggtaacgt agctatcctc cgccccggta aagcgagaga ccagctcgcc ggttttgtcc
     4921 accagctggt caatctccac gaaagaggat gccgcgcgcg cggtggcctg catcacctcc
     4981 ggcgcgacgc tggagacgcc gagaatcgtc atcttgccgc aggcgttaat cacctgtttc
     5041 agtcccagtt gttgatagat attctgcgtc atggcttaca gcactcccag cagcgagcag
     5101 accacgctca acgccacaat cgacagcaga atcgtggtgt aacgcggacc ctttttcacc
     5161 aaatagaagt agatggcgaa cactgccgcc agcggcagca ggccgggcgc gatggagtcc
     5221 agaatctgct gtaccaccac ttcagagcct ttcagtgcgc taatttttaa aggtgaggtt
     5281 atcttgacaa agctcgcgga tagcgcgccc atcatgataa gccccagtac gttagcgccg
     5341 tagataagct ccttgatgcg ccctccctgc agcagactga taatcgaatc gcgccccagc
     5401 gtatagccct tgtgcaccat gccgtagctg attgccagcg taatcgccgg gtagagaatc
     5461 agcggcaaaa taccgcccat cgcgctgcca ttggcggcga acgggataaa aatagcaatc
     5521 aacagcggca tcaccgccgc ccagatgatg gagtcgccca tcccggccag cggccccatc
     5581 aggccggttt taatcccggt gatagaggca tcgttaatcg cctcgccgcg ggtcttttgc
     5641 tcttccatgg cgatggagat cccctggatc accgcgccaa acgtctgctc tgagttgaag
     5701 aagttcagat ggcgttttaa cgcctccacc tgctcctcct tttgcggata gagctttttg
     5761 ataatcggcg tcatcgaggc gcagaaaatc aggctctgta aacgctcata ggagctggaa
     5821 acttccgcgc ccagccagta gataaaccac gctttggtga tatccgcttt ggtcagcgcc
     5881 ccggactccc gcgcgcgctc aaccagttca tgctgcatta cgtcgtcgct cattatgccg
     5941 ctccttcatt tttcgccagg cctttaatca ggaaggccac gcaggtgccg aaaatcgcca
     6001 tcgccatgat gtccaccttg aggtacagca ccgcgaagaa tccgccgata aaccacggca
     6061 gcaggctctt tttaccaatc accataatgg tgatggcgaa gcccagcgcc gggagaatcc
     6121 cgcccatgat ttcaaacgag tgggtcagcc agtgcggcat cagcttgagg aaactttcca
     6181 ctacgttctg gccgaagtag ttggcggcga acaccactgg gaaacgtagt accagcccca
     6241 gcagcgccgg gtagagaaag gcgcagcgca taatggctga catattggcc gtttccgcgt
     6301 gtttatcggc catatgcacc caggccgcat ttagcgtgcg acgcaactgg tcgaggaata
     6361 cgccgataac gccaaatgga atcgccagtg caatcgccag gttcgggtcc atattggcct
     6421 taacggcgat aggaatggcg atacaggccg ccagcgccgg gtctgacggc acattgccgc
     6481 ctggcgttga ggtcacaccc agatagacca actgcatccc cgcgccgata atcatcgccg
     6541 caggcatatt gccgagcagc aggccgacaa agaccgccac caccactggc tgtaacagca
     6601 tggcggagaa ggtatagcca aggcgcaacc gggcaaacca gtaatacagc cccatcaggc
     6661 ttgcaaaaag taaggtatcc ataatttgtt atcccatgtt cagagggtca gaattttttc
     6721 aggatatcgt ccagcgactg cggtttatct tccgggatgg tctggaaaac agccttaatg
     6781 ccgcgacttt tcaaatcgtt aagaatgcca acgtcttttt catccagcgt tatgttctgg
     6841 aacaccgctt tacggtttgg cccgccgcct aaaccgccaa cctgaatatc agccacgtca
     6901 aagccctgct gcaccgcttc ctgaatcgcc gccagcgacg ggaacagcac cagcacgttg
     6961 ccgtcgccca gctggttctc tttccacgcg gccgcgaagc tctggttgct aaagcagtcc
     7021 actttgatat tcggcggcgc ggccatcagg tagatatttt tcataaacgg atcggcgtcc
     7081 agctcgtcgc tgaccaccgc aatacggttg gcctgagact gccctaccca cttcgtcacc
     7141 acctgtccgt ggataagacg gctgtcaatg cgacataaca cgatgtttgc catacgattt
     7201 tccttactgt tgtaattgac gaatatggga gacgacgtcc aggcaactgt tgcgcccagc
     7261 ttccaccaga tcggccacgc aggcggtcag tgggccatgt tcgcgcttgt ccagcgcctc
     7321 cagcagcagc gaggcattaa gcccggagat caccgccacc ggataatcgg cgctcagccg
     7381 cgccgccacg ttagaggtgg tgccgccgat aaaatcggtc acgataagcg aaccttccgg
     7441 catggttttc accacggctt cgacgcgctg gtaaaactcg cccagcgtat cgacgggcat
     7501 cagcgcgatt tccgtcacgc cttcgatttc gcccatcacc atgcgcagac tgttgcagag
     7561 ctgcagcccc cagccgccgt gagttagcag cagaatttca ggtaacggat gaataggtac
     7621 agttgtggtc aaaatagcct cctttgctcc agtgtttaca tttcatcagg agcaaaggac
     7681 gtgccagaat ttgtgtcacg cacagaggaa atgagattgg atggggcgtt aagccccgta
     7741 aaccagggga tcagctgtag agcagttcgt agatataaaa atattctgcg tccgataaac
     7801 ggatagcgta agcctcttca atcggcaaaa aggaggattt gatgacgcta aaggcacgag
     7861 cgtccagatc aagccggttt tctagcgcca tctgtaacgg tttgcggtta atcacgatac
     7921 gctcgaccat gcagcagcag tggatcagaa aacgtagtgt cacctggcgg ctcggtttca
     7981 gggagagcgt gttggttaag tagttcagaa catcctcgat ctctttcaga attcgctgcg
     8041 ggttgagcac cgaaatatgg ttgatgatgc tctccatggt cagcgcgctg ataaagcgca
     8101 tggcgctgcg ctccatctcc agtcggcgct cgctgcttga gagatccggc gtcagcaggc
     8161 tgagcaccag ctccggccct tgctcggaaa acagttcctc cagcgagata aacggtatat
     8221 ccggcagtcc cggctggaag gtgccgacga tccctgccag ccgttcactg gcattaagcg
     8281 cctgctgaat acgctccagg ctgcgcacct cgttatagtc gagaatcacc atacgcgtgt
     8341 cctgcgacat cagctcgcca aagctctcct ccagcacctt tttgatcttt tccgccgtcc
     8401 ccattccggt tatacatgag ataaccagca ctttgccacc gctctcttgc tgcggcgtgc
     8461 agagccgaca agggatattt ttactctgca taagcacagc caattgcgat agatcgccag
     8521 tttcgtagct taaatccagc ccaacctcca gcaggctggt cagagtgata tttggcaaca
     8581 acagaacgtc aatctggaac agcttgctga tggtgctgcc aaaatggacc agcgatccga
     8641 tatccaccat cagtatcagc cgctgccagc accggtcctg aatcatccgg gtcagggttt
     8701 ccagggtgtc atgcaccgac tgctcaaatg gcatatcaat tgcgtagaac agatcgcgct
     8761 ccagcacccg gttcacatat tgcgcctggc tcgttgcggt ggtcgcaccg tgagcgatta
     8821 gaatcacgcc gcaatccggg ctggtgtcga tgcgctggcg gtagtggcga cactctttca
     8881 ggaacaggca cagccagacc acctccgtcg ccgggcactg aatatgcaac aactcattga
     8941 ttttgcggca cagaagcgtg gcattgtcat actcatcttt acagcgatcc agaatcaggc
     9001 tggatgaata gagttgaggt ataagccctc gccgcacata gccaatcagc gcaagaaagt
     9061 gtttacgtaa cgggttcgcc aggttttctg gcaatgtaaa tcctaatacc tgctccacgt
     9121 aaccgatcag cagcgtcaca cgctcctcaa tttgatcgcc atagcgcggt ggatgcgccg
     9181 cactatggcg gctataaaga ccatattcaa aaatcgaact gagtttgttt ttcaggatag
     9241 ccagcgtttc tgcgggcggc acgttactgt tgcgcagatt gacatattca cgagtcagga
     9301 aactgtaaaa caggtcgctc tcttctattt ccgcgctggt cgccaacgaa ttttttaacg
     9361 cgggcaacgt acgcgcatcc acgttcagac gatctttacc gccaaacaac gcatcgacca
     9421 gcagacgctg ctctggcgtg gtattaaacg acatctccgc cagtcgttta tctaactcta
     9481 gcgtatcatt acgctcggtc attcccgacg cccacgcctg cgcgcacaag aactgaatat
     9541 cacttttaag ctggccgata ttgccttcca ggggtttact taacaaccaa tgcaagagcg
     9601 ttttatcaag gcttatggta cgttcaattt tacgactttc acgctgaaag aagcttacaa
     9661 tcaattcgag ctgctcctcg accgaacgct ggcgaatacc cggtaagtca atacatacct
     9721 ggatacgccg ctggaaggta cgcaatagtg ccgagttgac cgactctgta gtggcgcaaa
     9781 taaggcgcac cgaaattgaa tatgttggcc cacttgaacc aagtgggcgg tattcacctt
     9841 tatcgagaat agaaaagagc ttctcttgtc cttcgtacgg gaggcgatgc acttcatcca
     9901 gaagtaaata acctccgtca gcttgctcca ccaatcccgt tttattctca cttgccccgg
     9961 taaatgcccc cttatggtga ccaaacagat gtgaggaaag caactccgga ttatgagcat
    10021 attcggcaca gttaaagtaa accaacggag gtggcgaacc tatggtgtgc ttgcaagcga
    10081 agcaatgcat taactcagca aagaatgttt tgccaacacc agatgcaccg gttaacagca
    10141 catgtagtcc gtggggataa agcaccgctg cgcggccttt ttctaccgca tcacggagac
    10201 tacggtcgta tccgataagt ccggaaaacg gatcatcatc tgtatatttt tctttctttg
    10261 gcaataaact agccactgaa tgaactatgc attcgcaatc atccagtttt cgattaatga
    10321 gcaattctag tgcgtgacga tgtagaaaaa aaacaggacg tccctgactt tttaccacca
    10381 ggccttcatt ccataattga ttaagatctt tactaaccga gttgcgtgcc agcccaagat
    10441 taaaaccaat cgcctcagca gtaaatgctt tctccttcgc taaatcagac agttccagcc
    10501 catacgtcag ccgctccagc tccttgtgaa taacatcaat ccgtctcatt tctttaccgc
    10561 ttaaaaaaca atagttgttc agaacataca ctatttgtaa acgtaagaat gagaactatc
    10621 gggaagaatt aatgttcctt gtggcaatct ggtttctgaa tattcgtgtt ttaaatatta
    10681 cttatggtct gaaacaacca ggcaggtgaa cagagctaag aaaaagtagc gtagaaatat
    10741 aaaaacatat atcgcactga tgaatgtttc ccggcatgac tatgcacacc gggaaatatt
    10801 tagtaaatta ctcgcccttt ttcgccgcct gaatatacag catctccagc gccagcgtcg
    10861 ccgcagccag agcagtgatt tccgactgat cgtatgccgg agccacttcc actacgtcca
    10921 tcccaacgat gttgagatct ttcaggccgc gtaccagttt aatggcgcga tcggaggtca
    10981 gaccgccaat taccggcgta ccggttcccg gcgcaaatgc aggatccagg cagtcgatat
    11041 caaaggtcag gtaaaccggc atatcaccca caatttgttt cacctgggcg ataacgtcat
    11101 ccacgctgcg atcgttcacc tggcaggcgt ccagcacggt aaagccgttg tctttatcaa
    11161 attcggtacg aataccaatc tgcacggaat gattcggatc gatcagacct tctttcggcg
    11221 cggtatagaa catggtgccg tggtcaaatt cacaaccgtt cgcgtaggta tcggtgtggg
    11281 cgtcaaagtg taccagcgcc attttgccga aatgcttcgc atgagcacgc agcagcggca
    11341 gcgtaacaaa gtggtcacca ccgaaggaga gcatacgctt accggcagcc agcagcttct
    11401 cggcgtgcgc ctgcagcttc tcgctcatct cacgggcatc gccgaaagca tataccagat
    11461 caccgcagtc cacgacgttc agacgctcac gcatgtcgaa attccacggg aagcggttgt
    11521 gttcccaggc cagattcgtc gaaacctgac ggatagctgc cggaccgtgg cgtccccccg
    11581 cacgaccaga agtggccata tcgaacggca caccagtaat cacccagtca gcatcgctgt
    11641 catacggctg gaagttcatc ggcaggcgta aaaaaccaaa ggcattggaa accagtgagt
    11701 tatcgtattg atgacctaag gtgctcatgg atggactcct attacaaagt cgatataaaa
    11761 aaacccttcc gcgtcgttag gcccgacgag gaagggttgg atttgcgaaa cacaagtttg
    11821 tgctaattat cgccttaaat gggcgaggca tcaagcgagg ttgctatttc cgacacggtg
    11881 gcggcgcata gccattgtta tctgccggaa aggcttgtcc agagcacggc ggcagcgcgt
    11941 tttgcgcctg gccttgtaat tcaaaggcgg caaagcctat tacaccggta ttacgcacat
    12001 cgccttgcgc cgaattggct gcgtaggaat cagcaacgtt ggcgaactga aaagccgctt
    12061 cggtatgttt atctttgcgg aatcctttaa tcgctaaaga atctccggca ttcacaatat
    12121 agccgttgtt attcagcgaa ccttgcttgc cgttcagcac atccaggcca tcaacagtag
    12181 caacgatctc gtagttggtg ttacgactgt aattacggac atataattga taattcattc
    12241 cgactctggc atggacaata tagcgtggtt cattattgga tgaatcataa acacgcgtaa
    12301 taggtagaga attaaaatta ccatcgcgaa tagcatattc cagttcgtta atacgaatgc
    12361 tataaacgcg gtcatagccg gaaggaacat tagtcgaata actaataacg atcatctctg
    12421 ccggatcgcg ccaggctcgc tcagcagata cgccctgaac cgatgagtgc acatccccac
    12481 cccaggcagt gccgacattg cccgatgcac tgctattata ctgcgcatta ctggaacaac
    12541 cggccagcag cacgattaat gccaccaacg cgctacgtac tttccatttt ttcataaccg
    12601 ttccctctgc atttaattaa tgcagcgatt gtagggagat tatttaataa gaaaagcagc
    12661 gagattattt ttaagaatat ataaaaagaa agcgggccga tttacgtaaa taaatcgacc
    12721 cgccagcgga ataatcttaa ttattcatct tcaagatagg tataaccgta caaacctgcc
    12781 tcgaactctt caaggaactg ttgttgcagt tcagcatcaa gatcggtttt cttcacttga
    12841 tcgcggaact gggttaacag cgttttcgga tcgagctgta catattgcag catgtccgcc
    12901 acggtatcgc cttcgtcaga cagttctact tctacgctac cgtcagggaa cacgaacacg
    12961 tcaaccgctt cggtatcacc gaacaggttg tgcatattgc caaggatctc ctgatatgcg
    13021 ccgaccataa agaaaccgag catcggcgga ttctctggat cgtactccgg cattggcatt
    13081 gtcgtggcaa taccatcacc atcaatatag tggtcgatag caccgtcaga gtcacaggta
    13141 atatccagca gcactgcgcg acgttccggc acttgatcca gcccttccag cggcagaacc
    13201 gggaacaact ggtcgatccc ccatgcatcc ggcattgact ggaacagcga gaaattgacg
    13261 tacattttgt ccgccatacg ttcctgcagt tcgtcgatta tcggacgatg cgcacggttt
    13321 tgcggatcca gctgcttttg cacttcatgg cacatgctca gatagagttg ttctgcccat
    13381 gcacgttctt gcaggctaaa ggtgccggaa gagtagccga tatgaatgtc gtgcagatcc
    13441 atctggctgt cgtgtaacca ttcacgcaga gaacggcgag ttcccggttc gtgcatttcc
    13501 tgccaggttt cccacatgtt ttgcagcgcg cgcggcgcat cttctgcagg cgcggtcggc
    13561 accgtgtatt cgttacgttc cacgccgatg atattagata ccagcacggt gtgatgcgca
    13621 gtcaccgcac gacccgattc ggtgattacc gtcggatgcg gcagaccgtt ttcttcacac
    13681 gcatcaccaa tcgcccagat gatgttgttg gcatattcat tgaggccgta gttcaccgaa
    13741 cagtcggact gcgaacgggt gccttcatag tccacgccca gaccgccgcc gacgtcgaag
    13801 cactgaatat tgacgcccag cttgtgcagt tccacataga aacgcgcgga ttcacgaacg
    13861 cctgtcgcga tatcgcgaat attcgccatc tgcgaaccga ggtggaagtg cagcagttgc
    13921 aggctgtcga gacgcccggc ttcacgcagg gtttcaacaa gttgcagtac ctgagtcgca
    13981 gccaggccga acttcgattt ttccccgccg gaggactgcc atttacccga gccctgcgaa
    14041 gccagacgtg cacgcacgcc cagacgagga acgacattca gacgttctgc ttcatccagc
    14101 acaatggcga tttctgacat cttctcaatg accaggtaga ccttgtggcc catcttctcg
    14161 ccaattaatg ccaggcggat atattcgcgg tctttataac cgttgcagac gatgacgcta
    14221 cgggtcatgc cagcatgtgc cagcaccgcc atcaactctg ctttcgagcc tgcttccagt
    14281 cccagcggtt cgcccgaatg aatcagggac tcaatcacgc ggcggtgctg gttaactttg
    14341 atcggataaa caaggaagta atcgccgtta tagccgtagg attcccgcgc acgtttgaac
    14401 gcggcgttaa tggaacgcaa acggtgctgc aggatctgtg ggaaacagaa cagtgcaggc
    14461 agacgctgac cctgtgcttc acgagttttc actaactgcg cgagatcgac gcgagcttcc
    14521 gggacgtccg ggtccgggca cacgctgatg tggcccagct cgttaacgtc atagtagtta
    14581 ttgccccacc aggcaatatt gtaagtacgc agcatcttgc tggcttcctg ggagctcatt
    14641 gcaacctcct gcatggagcg tagtacaccg tgttcgcccg ctgacgaagg caaacccata
    14701 gacatgtcgt cagacatagc gaacctcaaa ttattttatt aagtgtaaaa cagttaacga
    14761 ctatcgcagc ctggaactgc gataacaacc cataagcaca tggattttcc agcagtgtat
    14821 gctgacgctc cgactgcgca accggtttct ttttcataac attattaagc acataaccga
    14881 acgtaagtgt gacagttcgg cgaaaccacg agaaaactct tgtttttaca agagcgccct
    14941 tgttcagtcc tcagtaactg taaccagctc ttgaatcctg agaagcgccg agatgggtat
    15001 aacatcggca ggtatgcaaa gcagagatgc agagtgcggg gaacgaatct tcaccagaac
    15061 ggtgagacag gttaagcagc agacaacggt tcattatttc gtatcacctc cacggccgcc
    15121 tgttaagacg aacccacaag ccaaaactct gatttcaacc cggctggaag tggcaacacg
    15181 aaagaaacgt cgtgtgcttt ttatttaagc cgcgcgccgc gttttatacc ccacaatggc
    15241 agaaaattgc aaaagataaa tacgcagaat gccggcattg tcaggaaaaa tttccagcca
    15301 cgtttttaac agaatgagac acgattcaaa aaaaagtgga aatcgggtga agaattgacc
    15361 taaaatagcc atccagatgt taatccatcc ataccgatta acactcagac tgccagtgtt
    15421 tttaacttgc agagtcgtgg taggatccgc taccacagaa aatccacaca acagtttgag
    15481 ctaaccaaat tctctttagg tgatattaaa tatggcaaaa caccttttta cgtccgagtc
    15541 cgtctctgaa gggcatcctg acaaaattgc tgaccaaatt tctgatgccg ttttagacgc
    15601 gatcctcgaa caggatccga aagcacgcgt tgcttgcgaa acctacgtaa aaaccggcat
    15661 ggttttagtt ggcggcgaaa tcaccactag cgcctgggta gacatcgaag agatcacccg
    15721 taacaccgtt cgcgaaattg gctatgtaca ttccgacatg ggctttgacg ctaactcctg
    15781 tgcggttctg agcgctatcg gcaaacagtc tcctgacatc aaccagggcg ttgaccgtgc
    15841 cgatccgctg gaacagggcg cgggtgacca gggtctgatg tttggctacg caactaatga
    15901 aaccgacgtg ctgatgccag cacctatcac ctatgcacac cgtctggtac agcgtcaggc
    15961 tgaagtgcgt aaaaacggca ctctgccgtg gctgcgcccg gacgcgaaaa gccaggtgac
    16021 tttccagtat gacgacggca aaatcgttgg tatcgatgct gtcgtgcttt ccactcagca
    16081 ctctgaagag atcgaccaga aatcgctgca agaagcggta atggaagaga tcatcaagcc
    16141 gattctgccc gctgaatggc tgacttctgc caccaaattc ttcatcaacc cgaccggtcg
    16201 ttttgttatc ggtggcccga tgggtgactg cggtctgact ggtcgtaaaa ttatcgttga
    16261 tacctacggc ggcatggcac gtcacggtgg cggagcattc tctggtaaag atccatcaaa
    16321 agtggaccgt tccgctgcgt acgcagcacg ttatgttgca aaaaacatcg ttgctgctgg
    16381 cctggccgat cgttgtgaaa ttcaggtttc ctacgcaatc ggcgtggctg aaccgacctc
    16441 catcatggta gaaactttcg gtaccgagaa agtgccttct gaacaactga ctctgctggt
    16501 acgtgagttc ttcgacctgc gcccatacgg tctgattcag atgctggatc tgctgcaccc
    16561 gatctacaaa gaaaccgcag catacggtca ctttggtcgt gaacatttcc cgtgggaaaa
    16621 aaccgacaaa gcgcagctgc tgcgcgatgc tgccggtctg aagtaatctt tcttcacctg
    16681 ctttcaaagg ccagcctcgc gctggccttt ttcttttacc tcaccgcggt ggataggcat
    16741 tcacgccgca tccggcaaaa agctgcccgc acaataacat cattcttcct caacacgttt
    16801 tatcgcaaat tatcatcact actgaaaccg attacaccaa taacaccagc caaatatttg
    16861 taatattttc atattattat tcagttttca cagttgttac atttcttttc agtaaagtct
    16921 taattgcaga taacagcgtt taatctatga tgatataact caattatttt catgcactta
    16981 aaccataact aagataaatg ttagtgtaag cgattacact gatgtgattt gcttcacatc
    17041 tttttacgtc gtactcacct atcttaattc acaataaaaa ataaccatat tggagggcat
    17101 catgcctgac gctaaaaaac aggggcggtc aaacaaggca atgacttttt tcgtctgctt
    17161 ccttgccgct ctggcgggat tactctttgg cctggatatc ggtgtaattg ctggcgcact
    17221 gccgtttatt gcagatgaat tccagattac ttcgcacacg caagaatggg tcgtaagctc
    17281 catgatgttc ggtgcggcag tcggtgcggt gggcagcggc tggctctcct ttaaactcgg
    17341 gcgcaaaaag agcctgatga tcggcgcaat tttgtttgtt gccggttcgc tgttctctgc
    17401 ggctgcgcca aacgttgaag tactgattct ttcccgcgtt ctgctggggc tggcggtggg
    17461 tgtggcctct tataccgcac cactgtacct gtcagaaatt gcgccggaaa aaattcgcgg
    17521 cagtatgatc tcgatgtatc agttgatgat cactatcggg atcctcggtg cttatctttc
    17581 tgataccgcc ttcagctaca ccggtgcatg gcgctggatg ctgggtgtga ttatcatccc
    17641 ggcaattttg ctgctgattg gtgtcttctt cctgccagac agcccacgtt ggtttgccgc
    17701 caagcgccgt tttgttgatg ccgaacgcgt gctgctacgc ctgcgtgaca ccagcgcgga
    17761 agcgaaacgc gaactggatg aaatccgtga aagtttgcag gttaaacaga gtggctgggc
    17821 gctgtttaaa gagaacagca acttccgccg cgcggtgttc cttggcgtac tgttgcaggt
    17881 aatgcagcaa ttcaccggga tgaacgtcat catgtattac gcgccgaaaa tcttcgaact
    17941 ggcgggttat accaacacta ccgagcaaat gtgggggacc gtgattgtcg gcctgaccaa
    18001 cgtacttgcc acctttatcg caatcggcct tgttgaccgc tggggacgta aaccaacgct
    18061 aacgctgggc ttcctggtga tggctgctgg catgggcgta ctcggtacaa tgatgcatat
    18121 cggtattcac tctccgtcag cgcagtattt cgccatcgcc atgctgctga tgtttattgt
    18181 cggttttgcc atgagtgccg gtccgctgat ttgggtactg tgctccgaaa ttcagccgct
    18241 gaaaggccgc gattttggca tcacctgctc caccgccacc aactggattg ccaacatgat
    18301 cgttggcgca acgttcctga ccatgctcaa cacgctgggt aacgccaaca ccttctgggt
    18361 gtatgcggct ctgaacgtac tgtttatcct gctgacattg tggctggtac cggaaaccaa
    18421 acacgtttcg ctggaacata ttgaacgtaa tctgatgaaa ggtcgtaaac tgcgcgaaat
    18481 cggcgctcac gattaatctc ctcaggcttc ctcccatcgc ggaggaagcc acctcttgca
    18541 gtcatctttt cttcgctcta tcctctgccg ctatgaaaac atcccgtctc cctatcgcca
    18601 tccagcaggc cgttatgcgc cgcctgcggg aaaaactcgc ccaggccaac ctgaagctag
    18661 ggcgtaacta cccggagcca aaactctctt acacccagcg cggaacctcc gccggaacag
    18721 cctggctgga aagctatgaa atccgcctca atcccgtttt gctgttggaa aacagtgaag
    18781 cttttattga agaagtggta ccgcacgaac tggcacattt gctggtatgg aaacatttcg
    18841 gccgcgtagc gccacatggc aaagagtgga agtggatgat ggaaagcgtg ctgggcgttc
    18901 ccgcccgtcg tacgcatcag ttcgaactgc aatccgtgcg tcgcaacacc ttcccctacc
    18961 gctgcaagtg ccaggagcat cagcttaccg tacgccgcca taatcgcgta gttcgtggcg
    19021 aggccgtcta tcgctgtgtt cactgcggtg aacagctggt tgcgaaataa ccatctgaac
    19081 tatcaggaac tttcctgatc tggctgattg cataccaaaa caactttcgc tacgttgctg
    19141 gctcgtttta acacggagta agtgatgtac cgttatttgt ctattgctgc ggtggtactg
    19201 agcgcagcat tttccggccc ggcgttggcc gaaggtatca atagtttttc tcaggcgaaa
    19261 gccgcggcgg taaaagtcca cgctgacgcg cccggtacgt tttattgcgg atgtaaaatt
    19321 aactggcagg gcaaaaaagg cgttgttgat ctgcaatcgt gcggctatca ggtgcgcaaa
    19381 aatgaaaacc gagccagccg cgtagagtgg gaacacgtcg ttcccgcctg gcagttcggt
    19441 caccagcgcc agtgctggca ggacggtgga cgtaaaaact gcgctaaaga tccggtctat
    19501 cgcaagatgg aaagcgatat gcataacctg caaccgtcag tcggcgaggt gaatggcgat
    19561 cgcggcaact ttatgtacag ccagtggaat ggcggcgaag gccagtacgg tcaatgcgcc
    19621 atgaaggtcg atttcaaaga aaaagctgcc gaaccaccag cgcgtgcacg cggtgccatt
    19681 gcacgcacct acttctatat gcgcgaccaa tacaacctga cactctctcg ccagcaaacg
    19741 cagctgttca acgcatggaa caagatgtat ccggttaccg actgggagtg cgagcgcgat
    19801 gaacgcatcg cgaaggtgca aggcaatcat aatccgtatg tgcaacgcgc ttgccaggcg
    19861 cgaaagagct aacctacact agcgggactc tttttgttaa cccctacccc acgcgtacaa
    19921 ccgcgtgggg agacgacgcg gatttttaac tatgcgtatc ccccgcattt atcatcctga
    19981 accactgacc agccattctc acatcgcgct ttgcgaagat gccgccaacc atatcgggcg
    20041 cgtactgcgc atggggccgg ggcaggcgtt gcaattgttt gacggtagca accaggtctt
    20101 tgacgccgaa attaccagcg ccagcaaaaa aagcgtggaa gtgaaggtgc tggaaggcaa
    20161 gatcgacgat cgcgaatctc cgctgcatat tcacctcggt caggtgatgt cgcgtggtga
    20221 aaaaatggaa tttacgatcc agaaatcgat cgaactcggt gtaagcctca ttacgccact
    20281 tttttctgag cgctgcggcg ttaaactgga tagtgaacgt ctgaacaaga agctccagca
    20341 gtggcagaag attgcaattg ctgcctgtga gcagtgtggt cgtaaccggg tgccggaaat
    20401 ccgcccagcg atggatctgg aagcctggtg tgcagagcag gatgaaggac tgaaactgaa
    20461 tcttcacccg cgcgccagta acagcatcaa tacgttgccg ttaccggttg aacgcgtccg
    20521 cctgctgatt ggcccggaag gcggtttatc ggcagatgaa attgccatga ctgcccgcta
    20581 tcaatttact gatatcctgt tgggacctcg cgttttgcgt acagagacaa ctgcgctcac
    20641 cgccattacc gcgctacaag tacgatttgg cgatttgggc taacggagaa gaataatgat
    20701 caagctcggc atcgtgatgg accccatcgc aaacatcaac atcaagaaag attccagttt
    20761 cgctatgttg ctggaagcac agcgtcgtgg ttacgaactt cactatatgg agatgggcga
    20821 tctgtatctg atcaatggcg aagcccgcgc ccatacccgc acgctgaacg tgaagcagaa
    20881 ctacgaagag tggttttcgt tcgtcggtga acaggatctg ccgctggccg atctcgatgt
    20941 gatcctgatg cgtaaagacc cgccgtttga taccgagttt atctacgcga cctatattct
    21001 ggaacgtgcc gaagagaaag ggacgctgat cgttaacaag ccgcagagcc tgcgcgactg
    21061 taacgagaaa ctgtttaccg cctggttctc tgacttaacg ccagaaacgc tggttacgcg
    21121 caataaagcg cagctaaaag cgttctggga gaaacacagc gacatcattc ttaagccgct
    21181 ggacggtatg ggcggcgcgt cgattttccg cgtgaaagaa ggcgatccaa acctcggcgt
    21241 gattgccgaa accctgactg agcatggcac tcgctactgc atggcgcaaa attacctgcc
    21301 agccattaaa gatggcgaca aacgcgtgct ggtggtggat ggcgagccgg taccgtactg
    21361 cctggcgcgt attccgcagg ggggcgaaac ccgtggcaat ctggctgccg gtggtcgcgg
    21421 tgaacctcgt ccgctgacgg aaagtgactg gaaaatcgcc cgtcagatcg ggccgacgtt
    21481 gaaagaaaaa gggctgattt ttgttggtct ggatatcatc ggcgaccgtc tgactgaaat
    21541 taacgtcacc agcccaacct gtattcgtga gattgaagca gagtttccgg tgtcgatcac
    21601 cggaatgtta atggatgcca tcgaagcacg tttacagcag cagtaaccca ccttagcgag
    21661 aaggatctcg ttgagactct gagtgacagc gcccttcttt ccacgcatac tgggcgctgt
    21721 tgcttttttg aaccaggaaa cagaacctct gacaatgaat ttacagcatc actttcttat
    21781 tgccatgcct gctctccagg atccgatttt ccgtcgttcc gtggtctaca tttgcgaaca
    21841 taataccaat ggtgcaatgg ggatcatcgt caacaagccg ctggaaaatc tcaaaattga
    21901 agggattctg gaaaagctga agatcacgcc ggagccgcgt gatgaatcaa tccgtctgga
    21961 taaaccggtt atgctcggcg gtccgctggc tgaagatcgc gggtttattt tgcatactcc
    22021 gccctccaat tttgcttcca gcattcgcat ttcagacaac acagtgatga ccacttcccg
    22081 cgatgtgctg gaaacgctcg gcaccgataa acaaccgtct gacgtattgg tggctctggg
    22141 ttatgcctcc tgggaaaaag gtcaactgga acaagaaatt ctcgataacg cgtggctaac
    22201 ggccccggcg gatctgaata ttctgttcaa aacgccgatt gccgaccgct ggcgcgaggc
    22261 ggcaaaactg attggtgtgg atattctcac catgcctggt gtggcagggc acgcctgatg
    22321 agtggaacct tactcgcctt cgacttcggc accaaaagca ttggcgtagc ggtcggccaa
    22381 cgcattaccg gcactgctcg ccctttgcct gcaattaaag cacaggacgg tacgccggac
    22441 tggaacctca tcgaacgttt actgaaagag tggcagccgg acgaaatcat cgtcggtttg
    22501 ccgctgaata tggacggcac cgagcaacca ttgactgcca gagcgcgtaa atttgccaac
    22561 cgtattcatg gtcgtttcgg tgttgaagta aagctccatg acgaacgtct tagcaccgtg
    22621 gaagcccgtt ccggtctgtt tgaacagggc ggctatcggg cgctcaacaa aggcaaagtc
    22681 gactctgcct ctgcggttat tattctcgaa agctatttcg agcagggata ttaaggcgat
    22741 ctaaaagcct ggcggatagt cgttaccgcc atcttgccgg atgcggcgtg aacgccttat
    22801 ccagcacaca tctggcagcg gctgtaggtc tgataagacg cgttagcgtc gcatcagaca
    22861 tttattgcct ttataggccc tataaacttg cgcatcgggc atggcaacgt cacaaacgcc
    22921 cttcccccac ccgctgctga taactctgct gaaacgttaa catccccacc tgctgcccgg
    22981 tttgaataac atgcggtaac tggtgggttt tcccttcgcg aatcaaattc cccaccgcgg
    23041 gagtgttaat caataattca aacagcgcca cgcgtccttc ctgtttatcc acttccagct
    23101 tttgtgacaa cacggcccgt aaactacctg ccagttgatt acgtacggga tctttttcct
    23161 gcgccggaaa tgaatccacc agtcgctcaa ctgcctgcgc ggcaccacgc gtatgtaatg
    23221 ttgccagcac caaatgcccg gtttctgccg ccgtcagtgc cagacggatt gtctcgctgt
    23281 cacgcagctc tccgagcaaa atcacatcag gatcttcccg caatgcggcc cgcaatcccg
    23341 atgcgaacgt catacagtgc aaaccaattt cccgctgctg gatcaaacat cgctggctgg
    23401 catagatata ttcaacagga tcttccagcg tcagaatatg cgcatcggca tgttgattga
    23461 gatagccaac catcgccgcc agcgtggtag atttgccgct ccccgtcgcc cccgtcacca
    23521 gaatcaggcc attctcgctc ttgagtaatt ccggcaatac cgttggtgcg ccaagctgtt
    23581 cgagctgtgg acagtgcgaa ggtaacaacc gtaatgccag agaaatacct tgccgttgcg
    23641 cgaacgcact gccacgcaac cgctggtttt ccgccagcga cacggcaaaa tccagctggc
    23701 cattttccag caatattgcc cgctgatcgt catccagcca ctcccgcagt agctcttcga
    23761 cgtccggcgc atcaaacggc gcagcttcca ttagcccgcg aatgcgccat cgtgcggacc
    23821 aggcgctgca caggtgtaga tccgagacgt tatgctttac actaagggcc acaatttctt
    23881 ccatattcat actaagatcc tcggaaaatg aacgatattg cgcataacct ggcacaggtc
    23941 cgggacaaaa tctcagcggc tgcaacgcgt tgcggccgtt ctccagaaga aattacgctg
    24001 cttgcagtca gtaaaacaaa acctgcgagc gccatcgcag aagccattga tgccgggcag
    24061 cgtcaatttg gtgaaaacta cgttcaggaa ggggtagata aaattcgcca ctttcaggaa
    24121 ctgggcgtaa caggattaga atggcatttt attggcccgt tgcagtctaa taaaagccgc
    24181 ctggtggcag agcatttcga ctggtgtcat accatcgacc gtttgcgcat cgctacccgc
    24241 ctcaacgatc agcgcccggc agaacttccc cctcttaacg tactgattca aattaatatt
    24301 agtgatgaaa acagtaagtc cgggattcaa ctggcagaac tggacgagct ggcagctgcg
    24361 gtcgctgaac taccgcgttt acgtctgcgc gggctgatgg caatccctgc acctgagtca
    24421 gaatatgtaa ggcagtttga agttgcacgc caaatggctg tagcatttgc cggactgaaa
    24481 acgcgctacc cgcatatcga cacgctctct ctgggaatgt cggacgatat ggaagccgcc
    24541 attgcggcag gtagcacgat ggttcgtatc ggcactgcaa tttttggtgc gcgtgattac
    24601 tctaaaaaat aaggaattaa aggaacgcca tgaatacgtt gactttcctg ctttcaacgg
    24661 tcattgagct gtataccatg gtgctgttat tacgcatctg gatgcagtgg gctcattgtg
    24721 atttttacaa ccccttctca cagtttgtag tgaaggtaac gcagccaatt atcgggccac
    24781 tgcgccgcgt tattccggca atgggcccca ttgacagcgc ctcgctgctg gttgcctata
    24841 ttctcagttt tatcaaagcc atcgtgctgt ttaaagtggt gaccttcctg ccaatcatct
    24901 ggattgccgg tttactgatt ctgctgaaaa ccatcggcct gctgattttc tgggtcctgc
    24961 tggtgatggc gattatgagc tgggtaagcc agggtcgtag cccgattgaa tacgtgctga
    25021 ttcagctggc cgatccgctg ctgcgcccga ttcgccgcct gctaccggca atgggtggga
    25081 ttgatttctc gccgatgatc ctcgttctgc tgctgtatgt catcaatatg ggtgtcgcag
    25141 aagtattaca ggcaaccgga aacatgctgc tgccggggct gtggatggcg ttatgagtgc
    25201 cgtaacagtt aatgatgacg gtctggtttt acggctctat attcagccga aagccagccg
    25261 tgattctatt gtcggtttac atggcgacga agttaaagtc gccattaccg cgccgccggt
    25321 tgacggtcag gccaacagtc atctggtgaa gtttctcggt aagcaatttc gggttgccaa
    25381 aagccaggtg gtgattgaaa aaggcgaact tggccgccac aaacaaatta aaatcattaa
    25441 tccgcaacaa atcccgccag aaatcgcggc gttaattaat taggtatcct atgcaaaaag
    25501 ttgtcctcgc aaccggcaat gccggtaaag tgcgtgagct ggcgtcgctg cttagcgact
    25561 tcggtcttga tatcgtggcc caaacagacc tcggcgtaga ttccgctgaa gaaaccggcc
    25621 tgacctttat cgaaaacgcg attctgaaag cgcgccatgc ggcaaaagtg accggtttac
    25681 cggcaattgc cgacgactct ggtctggcgg tagatgtgct tggcggcgcg ccgggcattt
    25741 actccgcgcg ttattccggt gaagacgcga ccgatctaaa gaatctgcaa aaactgctgg
    25801 aaacactgaa agacgtaccg gatgaccaac gtcaggcgcg tttccactgc gtgctggtat
    25861 atctgcgtca cgcggaagat ccgactccgc tggtatgcca cggtagctgg ccgggcgtga
    25921 ttactcgtga accagcgggc actggtggct ttggttatga tccaatcttc ttcgtacctt
    25981 ccgaagggaa aaccgccgcc gaactgaccc gcgaagaaaa gagcgccatt tcccaccgtg
    26041 gtcaggcatt gaaactgctg ctggacgctt tacgtaatgg ttaaattacc gccgctgagt
    26101 ctctacattc acatcccgtg gtgcgtgcag aaatgcccgt actgcgattt caactctcac
    26161 gcgttgaaag gagaagtgcc gcacgacgat tacgttcagc atctgctttg cgatctggac
    26221 aacgatgtgg cttacgctca gggccgtgaa gtaaagacaa tctttattgg cggtggtacg
    26281 ccgagcctgc tttccggccc ggcgatgcaa acgctgctgg acggcgtgcg tgcgcgtttg
    26341 ccgctggcag cggatgcaga aattactatg gaagcgaacc ctggtacggt agaagccgat
    26401 cgctttgtcg attatcagcg tgctggtgtg aaccgcatct ctattggtgt gcagagtttt
    26461 agcgaagaca agctgaaacg acttgggcgt attcatggcc cgcaagaagc gaaacgcgcg
    26521 gcaaagctgg cgagcgggct ggggctgcgt agctttaacc tcgatttgat gcatggacta
    26581 ccggatcaat cactggaaga ggcgcttggc gatctacgcc aggccattga actgaatccg
    26641 ccgcatcttt cctggtatca actgaccatc gaacctaata cgctgtttgg ttcacgcccg
    26701 ccggtactgc cggacgacga cgcgctgtgg gatatattcg aacaggggca tcagttatta
    26761 accgcagcgg gttatcagca atatgaaact tccgcttacg ccaaatccag ttatcagtgc
    26821 cagcacaatc tcaactactg gcgctttggc gactacatcg gtattggctg cggcgcgcac
    26881 ggcaaagtga ccttcccgga tgggcgcatt ctgcgtacca ctaaaacgcg tcatccgcgt
    26941 ggttttatgc aaggaaggta tctggaaagc cagcgtgatg tcgaagccgc agataagccg
    27001 tttgagttct ttatgaatcg cttccgtctg ctggaggccg cgccgcgcgt ggagtttatt
    27061 gcgtataccg ggctttgcga agatgtgatt cgcccacagt tagacgaggc gattgcccag
    27121 ggttatctca ccgaatgtgc ggattactgg cagataacgg aacatggtaa attattctta
    27181 aactcacttc ttgaattatt cctcgctgag taatcattaa tgcgtctgcg ggcgcattaa
    27241 tcacttcttc cccccccccg ttaattaact tcggcaaaat atatttctta c
//
LOCUS       DAZGTR010000056        27232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGTR010000056 DAZGTR010000000
VERSION     DAZGTR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.056"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            91..291
                     /locus_tag="ACHLCY_002591"
     CDS             91..291
                     /locus_tag="ACHLCY_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFP0369824.1"
                     /translation="MTTTTDKNQWCGQFKRCNGCKLQSECMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIARELGKLNNKAA"
     gene            357..827
                     /locus_tag="ACHLCY_002592"
     CDS             357..827
                     /locus_tag="ACHLCY_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369825.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTDHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSIPDIEYNFGHNQETENWVFY"
     gene            1022..1192
                     /locus_tag="ACHLCY_002593"
     CDS             1022..1192
                     /locus_tag="ACHLCY_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369826.1"
                     /translation="MQQFAIAGAASVRPFNPILSVQHSRKNILTGADFKQPRMKSLLE
                     KLWDILKQQGRP"
     gene            1268..1438
                     /locus_tag="ACHLCY_002594"
     CDS             1268..1438
                     /locus_tag="ACHLCY_002594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369827.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            1449..2054
                     /locus_tag="ACHLCY_002595"
     CDS             1449..2054
                     /locus_tag="ACHLCY_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HFP0369828.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGNRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            2054..2437
                     /locus_tag="ACHLCY_002596"
     CDS             2054..2437
                     /locus_tag="ACHLCY_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369829.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YNAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     ASGNGPQQQSSNQQNTPVYDDSIPF"
     gene            2461..2754
                     /locus_tag="ACHLCY_002597"
     CDS             2461..2754
                     /locus_tag="ACHLCY_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFP0369830.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCVWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISNN"
     gene            2765..2932
                     /locus_tag="ACHLCY_002598"
     CDS             2765..2932
                     /locus_tag="ACHLCY_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFP0369831.1"
                     /translation="MRGLAYNPSILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMLRKGGKQ"
     gene            2929..3228
                     /locus_tag="ACHLCY_002599"
     CDS             2929..3228
                     /locus_tag="ACHLCY_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369832.1"
                     /translation="MSVCLIDKRRRGQQIPSVEMPNHTWFCVLDIDGMDTLVDTRHYC
                     DTATATPAKAKKIAALIENWTPPDGWCNGNDRDWHEKMKGYICDFLRKCNGFRVM"
     gene            3230..3784
                     /locus_tag="ACHLCY_002600"
     CDS             3230..3784
                     /locus_tag="ACHLCY_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0369833.1"
                     /translation="MNKIDYQALRAKAEKATCGEWSLEYGEERFDAGDALIHREVVGY
                     LPICRIEGAHPESGFDEDFQMEQQANAEFIAAFNPKVALALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELEAEKQRAKVLFMENARLKSGIAGLIHLGIRYADVEVMKIAGDA
                     QLSTPCTDSIINSIATGIRIKGGE"
     gene            3784..3960
                     /locus_tag="ACHLCY_002601"
     CDS             3784..3960
                     /locus_tag="ACHLCY_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369834.1"
                     /translation="MRVACIGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     SARRAAKKRRRAKR"
     gene            3957..4145
                     /locus_tag="ACHLCY_002602"
     CDS             3957..4145
                     /locus_tag="ACHLCY_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369835.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRVGLMKHYGMMDCGFCK
                     KPSAAQNRWKNHLRTKGE"
     gene            4147..4356
                     /locus_tag="ACHLCY_002603"
     CDS             4147..4356
                     /locus_tag="ACHLCY_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369836.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            4461..5156
                     /locus_tag="ACHLCY_002604"
     CDS             4461..5156
                     /locus_tag="ACHLCY_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369837.1"
                     /translation="MLRLWDDLNDRYAPPEVVRELARIALASLEAKPIGAFHIAEQQV
                     DGTSDYLKDGEWPIDNGTIEVYAAPPVPVVPEEKPMPNPLSMYAVDAVAAIAEVRGWN
                     ACRAAMLHGAEPVSQTYKSQHTQFEQVADLYEMQFDDGRTCAFHTDAQKAAQWLQACD
                     GNRVQEYVKLERLRNALSGNSPVTPDGWVMVPKEPTQAMIKAWLSEIANFRGHAAGYR
                     AALEAAPQEVNRG"
     gene            5149..5433
                     /locus_tag="ACHLCY_002605"
     CDS             5149..5433
                     /locus_tag="ACHLCY_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0369838.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEIKTITHPHFGDKPVKVFAINVNIGNE"
     gene            5505..5804
                     /locus_tag="ACHLCY_002606"
     CDS             5505..5804
                     /locus_tag="ACHLCY_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369839.1"
                     /translation="MVCSTFNHLTLQKYQPDPEDLCSLCGGNHGKAAMIECNDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            5886..6077
                     /locus_tag="ACHLCY_002607"
     CDS             5886..6077
                     /locus_tag="ACHLCY_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0369840.1"
                     /translation="MTDTSLIPEKEVMNKLGVSSRQTIWNYTKRHGFPKPVRTHPKSY
                     LREAVEGWILNGGVNQKCS"
     gene            complement(6058..7236)
                     /locus_tag="ACHLCY_002608"
     CDS             complement(6058..7236)
                     /locus_tag="ACHLCY_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0369841.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTTYNDVLFPGEDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTL
                     VTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD
                     "
     gene            7418..8842
                     /gene="sbcB"
                     /locus_tag="ACHLCY_002609"
     CDS             7418..8842
                     /gene="sbcB"
                     /locus_tag="ACHLCY_002609"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP0369842.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEATFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYAEEIQMLA
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(8885..9112)
                     /gene="tsuB"
                     /locus_tag="ACHLCY_002610"
     CDS             complement(8885..9112)
                     /gene="tsuB"
                     /locus_tag="ACHLCY_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP0369843.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(9126..10184)
                     /gene="tsuA"
                     /locus_tag="ACHLCY_002611"
     CDS             complement(9126..10184)
                     /gene="tsuA"
                     /locus_tag="ACHLCY_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP0369844.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(10363..11721)
                     /gene="plaP"
                     /locus_tag="ACHLCY_002612"
     CDS             complement(10363..11721)
                     /gene="plaP"
                     /locus_tag="ACHLCY_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP0369845.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(11711..11773)
                     /gene="yoeI"
                     /locus_tag="ACHLCY_002613"
     CDS             complement(11711..11773)
                     /gene="yoeI"
                     /locus_tag="ACHLCY_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP0369846.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(11988..12917)
                     /gene="yeeY"
                     /locus_tag="ACHLCY_002614"
     CDS             complement(11988..12917)
                     /gene="yeeY"
                     /locus_tag="ACHLCY_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0369847.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEDWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAMEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(12963..13787)
                     /gene="yeeZ"
                     /locus_tag="ACHLCY_002615"
     CDS             complement(12963..13787)
                     /gene="yeeZ"
                     /locus_tag="ACHLCY_002615"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0369848.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTSRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(13870..14124)
                     /gene="yoeB"
                     /locus_tag="ACHLCY_002616"
     CDS             complement(13870..14124)
                     /gene="yoeB"
                     /locus_tag="ACHLCY_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP0369849.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(14121..14372)
                     /gene="yefM"
                     /locus_tag="ACHLCY_002617"
     CDS             complement(14121..14372)
                     /gene="yefM"
                     /locus_tag="ACHLCY_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP0369850.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            14656..14706
                     /gene="hisL"
                     /locus_tag="ACHLCY_002618"
     CDS             14656..14706
                     /gene="hisL"
                     /locus_tag="ACHLCY_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP0369851.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    14682..14805
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            14852..15751
                     /gene="hisG"
                     /locus_tag="ACHLCY_002619"
     CDS             14852..15751
                     /gene="hisG"
                     /locus_tag="ACHLCY_002619"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP0369852.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            15757..17061
                     /gene="hisD"
                     /locus_tag="ACHLCY_002620"
     CDS             15757..17061
                     /gene="hisD"
                     /locus_tag="ACHLCY_002620"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP0369853.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASENITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELTPQGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            17058..18137
                     /gene="hisC"
                     /locus_tag="ACHLCY_002621"
     CDS             17058..18137
                     /gene="hisC"
                     /locus_tag="ACHLCY_002621"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP0369854.1"
                     /translation="MSTDNFLSVTDLARKNVRELTPYQSARRLGGNGDVWLNANEYPT
                     AVEFQLTQQTLNRYPECQPKAVIENYAQYADVKPEQVLVSRGADEGIELLVRAFCEPG
                     KDAILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGIADKLDGVKVVYVCSPNN
                     PTGQLINPQDLRSLLDMTQGKAIVVADEAYIEFCPQATLAGWLSEYPHLVILRTLSKA
                     FALAGLRCGFTLANEEVINLLLKVIAPYPLSTPVADIAAQALTPQGISAMRKRVEQIL
                     EERQYLVDNLKTIRCVEQVFDSETNYVLARFTASSSVFKSLWDQGIILRDQNKQPSLS
                     GCLRITVGTREESQRVTDALRAEQV"
     gene            18134..19201
                     /gene="hisB"
                     /locus_tag="ACHLCY_002622"
     CDS             18134..19201
                     /gene="hisB"
                     /locus_tag="ACHLCY_002622"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP0369855.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPEVVPVLLKLQ
                     KAGFKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVTFDEVLICPHLPADECD
                     CRKPKVKLVEQYLVEQAMDMANSYVIGDRATDIQLAENMGISGLRYHRDTLNWAMIGE
                     QLTKRDRYAHVERNTKETQIDVKVWLDREGRSKINTGVGFFDHMLDQIATHGGFRMEI
                     AVKGDLYIDDHHTVEDTGLALGEALKLALGDKRGINRFGFVLPMDECLARCALDISGR
                     PHLEYRAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            19201..19791
                     /gene="hisH"
                     /locus_tag="ACHLCY_002623"
     CDS             19201..19791
                     /gene="hisH"
                     /locus_tag="ACHLCY_002623"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP0369856.1"
                     /translation="MNVVILDTGCANLHSVKSAVARHGYDPVVSRDPDVVLRADKLFL
                     PGVGTAQAAMDQLHERELIELIKACTQPVLGICLGMQLLGRRSEESNGVSLLGIIEQD
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFFGVQFHPERSGAAGAQLLKNFLEM"
     gene            19791..20528
                     /gene="hisA"
                     /locus_tag="ACHLCY_002624"
     CDS             19791..20528
                     /gene="hisA"
                     /locus_tag="ACHLCY_002624"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP0369857.1"
                     /translation="MIIPALDLIDGTVVRLHQGNYAKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAQRQIPQIKTLVAGVNVPVQVGGGVRTEADVAALLDAGVARVVVG
                     STAVKSPEIVKSWFARFGADALVLALDVRIDEHGNKQVAVSGWQEDSGVSLEELVETY
                     LPVGLKHVLCTDISRDGTLAGSNVALYEEVCAKYPQVAFQSSGGIGGIEDVAALRGTG
                     VRGVIVGRALLEGKFTVEEAIQCWQNA"
     gene            20510..21286
                     /gene="hisF"
                     /locus_tag="ACHLCY_002625"
     CDS             20510..21286
                     /gene="hisF"
                     /locus_tag="ACHLCY_002625"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP0369858.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYADEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSIDDAARILSFGADKISI
                     NSPALAEPELITRLADRFGVQCIVVGIDTWYDADTGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVRDVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            21280..21891
                     /gene="hisIE"
                     /locus_tag="ACHLCY_002626"
     CDS             21280..21891
                     /gene="hisIE"
                     /locus_tag="ACHLCY_002626"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP0369859.1"
                     /translation="MLTKQQCRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TLESGKVTFFSRTKQRLWTKGETSGHFLNVVSIAPDCDNDTLLVLVNPIGPTCHKGTS
                     SCFGDASHQWLFLYQLEQLLAERKTADPASSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(21948..22931)
                     /gene="wzzB"
                     /locus_tag="ACHLCY_002627"
     CDS             complement(21948..22931)
                     /gene="wzzB"
                     /locus_tag="ACHLCY_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP0369860.1"
                     /translation="MSISDNNMSGRSHDPEQIDLIDLLMQLWRGKVTIIICIVVAIAL
                     AVGYLAVAKEKWTSIAIVTQPDAGQLASYNNAMDVLFGANAPEMTDVQAGFIGRFSSA
                     FSALAETLDNQQEPEKLSIDAAVKGQTLPLKVSYTGKTSEEAQKTLAQYIQQVDEGVA
                     KELNADLSMSMETRLADLQKSLAAQEAVAKEQKTLRIAQMTQALKVAQQSNIKLPQVQ
                     QVDQVSQDSMFMLGSEALSSMVENEATRPLTFSDQYYQTRQNLLEVQALEVAPDSVHA
                     YRYVMKPTLPIRRDSPKKAITLVLAVLIGGMIGAGVVLGRNALRGYKAKAE"
     gene            23330..24334
                     /locus_tag="ACHLCY_002628"
                     /pseudo
     CDS             23330..24334
                     /locus_tag="ACHLCY_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
     gene            complement(24393..25559)
                     /gene="ugd"
                     /locus_tag="ACHLCY_002629"
     CDS             complement(24393..25559)
                     /gene="ugd"
                     /locus_tag="ACHLCY_002629"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP0369861.1"
                     /translation="MKITISGTGYVGLSNGILIAQHHEVVALDIVPTKVEMLNQKKSP
                     IVDKEIEEYLATKQLNFRATTDKYDAYRDADYVIIATPTDYDPKTNYFNTSSVESVIR
                     DVVDINPNAVMVIKSTIPVGFTNLLKERLGIDNIFFSPEFLREGRALYDNLHPSRIVI
                     GERSERAGRFAALLQEGAVKKDIPTLFTDSTEAEAIKLFANTYLALRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYASVPNNIIGAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKSGSDNFRASSIQGIMKRIKAKGVPVII
                     YEPVMVEDEFFHSRVVRDLTAFKNEADIIISNRMTSELADVADKVYTRGLFGSD"
     gene            complement(25758..27164)
                     /gene="gndA"
                     /locus_tag="ACHLCY_002630"
     CDS             complement(25758..27164)
                     /gene="gndA"
                     /locus_tag="ACHLCY_002630"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP0369862.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVVA
                     ENPDKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCITYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELATT
                     FTEWNEGELSSYLIDITKDIFTKKDEEGKYLVDVILDEAANKGTGKWTSQSSLDLGEP
                     LSLITESVFARYISSLKDQRIAASKVLSGPQAKLAGDKAEFVEKVRRALYLGKIVSYA
                     QGFSQLRAASDEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENKGIANLLLAPY
                     FKNIADEYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRSAVLPANLIQAQRDYFGA
                     HTYKRTDKEGVFHTEWLD"
BASE COUNT         7276 a         6754 c         6716 g         6486 t
ORIGIN      
        1 ccacttattt gaggtgagat atggaagaag aatttgaaga gttcgaagag catccgcagg
       61 atgtgatgga acaataccag gactatccgt atgactacga ctactgataa aaatcaatgg
      121 tgtggacaat tcaagcgatg caatggatgc aagctgcaat cggaatgcat ggttaagcct
      181 gaagaaatgt ttcctgtaat ggaagatggg aaatatgtcg ataaatgggc aatacgaacg
      241 acggcaatga ttgccagaga acttggtaaa ctgaacaaca aggctgcctg atggcggcct
      301 ttatttttgg cataaacaac agaataaaca cagcactgaa ttatttgagg tgagatatga
      361 caaaatcatg gagcgtacct tttcctgaat cagaaactga acatgatgga atgcctgttt
      421 tctggagatt ccaagcgaca gttgaagaag atggaatcaa aatattcgca cttcaatata
      481 tagcttttca tcagacagat cattatgcat ggttggttcc tgcgcattgg attgttaatt
      541 ttaaaccagc accaaatcag tggttacagg aatggaaaca aaggagaaat agatatgcaa
      601 ttaagaaagt agcaaaaaat gccgaaagat cttttgcatt cccgacgaag aaacttgcca
      661 ttgaaagttt attgcgccgg aagaagtacc atttaatgag aatcaaacaa gatttggctg
      721 ttgtatcaac tcttgttgat gggatgaaga atattgatac atcaatacca gatattgaat
      781 ataactttgg acacaaccaa gaaacagaaa attgggtgtt ttattagtac gaataagcac
      841 tgtgtattca ttccaacgag tgaatacacg gagcaatgtc gctcgtaact aaacaggagc
      901 cgacttgttc tgattattgg aaatcttctt tgccctccag tgtgagggcc tttttatatg
      961 cataccaata acgcttcact cgaggcgttt tcgttatgca atcaaacaga aggagcatcc
     1021 tatgcaacag ttcgctattg caggggcggc atcggttcgc cctttcaacc cgattttatc
     1081 ggtacagcat tcacgaaaaa atattttaac cggagcagac tttaaacaac caagaatgaa
     1141 aagcttgctc gaaaagcttt gggatatttt gaaacaacaa ggccgcccat gagttttaca
     1201 gataactggt cagacgaaga attcattcgt cagatgaaag aattaatcgg taacgaagga
     1261 gatattcatg tcacttgcaa ccaccgtgaa ggagagcaag ttacagagac gcatgtacac
     1321 gcagcaggcg ttaatgtatc gccagaaggg agatcgtgaa ggtgttcgcg tatttttaaa
     1381 tgcggcaaag actgaagtat taaatcagcg ttatttcctt gggccatgtc cattctgaga
     1441 acaatcatat gagcaaagaa ttttacgcaa gactggcagc tattcaggaa aatctgaacg
     1501 cgcccaagaa tcagtacaac tcattcggta aatataaata cagaagctgc gaagacattc
     1561 ttgaaggcgt taagccgtta ctgaatggtc tgtttttatc aatcagcgat gaagttgtgt
     1621 tgattggtaa tcggtattac gtgaaagcca cggcaactat taccgatggc gaaaacagtc
     1681 atacggcaac cgctcttgca cgagaggaag aaagcaagaa aggaatggat tctgcacaag
     1741 ttacgggagc tacaagctct tatgcacgca agtattgcct caatggtttg ttcggcattg
     1801 atgatgcgaa agatgcagat acagacgagc ataaacatca gcagaacgca gcagcaaagc
     1861 aatcaaaacc atcacctaca cctgaacagg ttctaaaagc attcactgac gcagcattgc
     1921 agaaaaacac cgtagaagag cttaaacagg cgttcgccaa agcgtggaag atgctcgaag
     1981 gcacaccgga gcagcacaaa gcgcaggacg tttacaacat cagacgagac gaattagaag
     2041 gagcggctgc ttaatggcac attcgattac tgtaagacta aacaagcccg caagagagtt
     2101 tcaggccggg gaaaatatcg gattcaacat ccgtgctggc gttcagtatt acgatcgcca
     2161 gacaaaaaag aaagaatgga caaactacaa cgccgttgta tttgccaagc cgggagcgca
     2221 agcggattac taccgtagtg ttcttgttga aggtggcatt gtagaaatta ccggagaaaa
     2281 catcagggtt gatgtttatc aggggcaaaa tggtcaatca atcactcttg aattactgaa
     2341 tgcaaagatt ggatttgcag cttcaggaaa tggcccgcag cagcaaagta gtaaccagca
     2401 gaacacccct gtatacgacg attccatccc attctgattt agaaaaataa ggatttaatt
     2461 atgccagcgc ctctgtatgg tgcggatgac ccgcgccgct gttccggcaa ttccgtatcg
     2521 gaggtgctgg ataaattcag gaaaaattac gaccggataa tgtcgctacc gcaggaaaca
     2581 aaagaggaaa aggaatttcg ccactgcgta tggcttgcag agaaagaaga acgcgagcga
     2641 atttaccaga catcaatccg accattccgc aaagccacat atacccactt ccctgaaatt
     2701 gacccgcgcc tgcgtaatta ccgctcacgc tatggcgcta tcagtaataa ctgaggaatt
     2761 taccatgaga ggacttgcat acaatcccag cattcttccg gcagaaatga ttattcgcca
     2821 gcgcgtaaag ccaatgccat cgagagagga attacttaag agaaattctt ttccgtcagt
     2881 aaatcaaaac aaatatctga atgcgatgtt gcgtaaagga ggcaagcagt gagtgtatgt
     2941 cttattgata aacgtcgacg tgggcaacaa ataccatctg ttgaaatgcc gaatcacaca
     3001 tggttttgcg tacttgatat cgatggtatg gatacgttgg ttgacactcg tcattactgc
     3061 gataccgcaa cagctactcc ggcgaaagca aagaaaattg ctgctctgat agaaaactgg
     3121 actccacctg atggttggtg caatgggaat gatcgagatt ggcatgaaaa aatgaagggc
     3181 tatatctgcg atttcttacg taaatgcaac ggattcaggg tgatgtgaca tgaacaagat
     3241 tgactatcag gcactgcgtg ccaaggcaga aaaagcaacg tgtggtgagt ggtcgctcga
     3301 atatggagag gagagatttg atgctggtga tgcgctaatt catcgtgaag ttgttggata
     3361 tcttcccatt tgcagaattg aaggagcgca tcctgaaagc ggtttcgatg aagatttcca
     3421 gatggaacag caggccaatg ctgaatttat tgctgccttt aatccaaagg ttgctttggc
     3481 actgctggat gaacgggaaa gaaaccagca atacatcaaa cgccgcgacc aggagaacga
     3541 ggatattgcg ctaacggtag ggaagctgcg cgttgagctt gaagcagaaa aacagcgggc
     3601 aaaagttcta tttatggaaa atgctcggct taagtcaggc atagccggtc tgatacacct
     3661 cggtattcga tatgcagatg ttgaggtcat gaaaattgct ggagatgccc agctttctac
     3721 cccatgcact gacagcatca taaacagcat tgcaacaggc attcgcatca aaggaggtga
     3781 gtaatgcgtg tggcatgtat cggcttgtta ccgtacccga ctcgtttttg ggcttctgcg
     3841 ctaattgcaa agccgcatgt cctgatggct gacaacatca tcccggcacc aaagcgccgc
     3901 cataccggta ttgcatcggc acgacgagca gcaaagaaac gcaggagagc aaaacgatga
     3961 aaaaccgtaa agcaaagatt ctgttagttc gtagaaacgc tcctggcgtc tggcagtggg
     4021 tgagactcag caaccgacgg gtggggttaa tgaaacatta cgggatgatg gattgtggtt
     4081 tttgcaaaaa gcccagcgcg gcgcaaaacc gctggaaaaa ccacttgcgt actaaaggag
     4141 agtgatatgg ctattgccgc aagttacacc atgcatctct attgtgactg ccgccagtgt
     4201 acggaaggtg tatatccagt gccagacttc ggagagtata tcggcacgtc atgggctggt
     4261 tgtgcaaaag aggcgcgtaa ggatggctgg cgaataagca aagacaaaac acgtgctttt
     4321 gcgcctgggc ataaagtttt gaggattaac aaatgaccac tattaccaga gaacaagctc
     4381 tgaaaattat tgaggcagcc gatgaggtta ttagtgcgct tgccggaact aacgaggatg
     4441 ttcaccctgg tagcgataac atgctacgcc tgtgggatga cctgaatgac cgttacgcgc
     4501 cgccggaagt tgtgcgtgag ctggcacgga ttgcgctggc atcgttggaa gcaaaaccaa
     4561 taggtgcatt ccacattgca gaacagcaag ttgacggcac aagtgactac ctcaaggatg
     4621 gagaatggcc tattgataat gggacaattg aagtatacgc cgcccctcca gtgccagtag
     4681 tacctgaaga aaaaccaatg cctaaccctc ttagcatgta cgcggtcgat gctgttgccg
     4741 ctattgcaga ggtgagaggc tggaacgcct gccgcgccgc catgcttcat ggtgccgaac
     4801 ctgtaagcca aacttacaag tcacaacaca cgcagtttga acaagttgct gacctctacg
     4861 aaatgcaatt tgatgacggt cgcacttgtg cctttcacac tgatgcgcaa aaggctgcgc
     4921 aatggcttca ggcatgcgac ggaaacaggg ttcaggaata cgtgaagctg gagcgattgc
     4981 gtaatgcgct atcgggcaac tctccggtaa ctccggatgg ttgggttatg gtgccgaagg
     5041 aaccaactca ggccatgatt aaagcgtggc tatcagagat tgctaacttt cgcggtcatg
     5101 ctgctggtta cagggctgct cttgaggcag caccacagga ggttaaccgt ggctaacctg
     5161 caacttgccg tcaaaggtga atacttcgat gccatgattc gcggagagaa aacagaagag
     5221 tatcgcctgt gtaatgacta ctggaataag cgaattatgt tccgggagta tgaccgcctg
     5281 attatcacaa agggatatcc gaagcgcgac gactccagcc gcagaataga cgtcccgtat
     5341 gacggatatg aaatcaagac aatcacacat ccccacttcg gcgataaacc ggtaaaggtg
     5401 ttcgcgataa atgtgaatat cggcaatgaa taacaaccct cgcatttgcg gggatttctt
     5461 ttatctgaac tcgctacggc gagttttgtt ttatggagat gattatggtc tgttcaacat
     5521 tcaaccatct aacgttacag aaataccagc cagaccctga agatttatgc tcactgtgtg
     5581 gcggaaatca tggtaaagcc gccatgatcg aatgtaatga caaaatccac atttgcctta
     5641 attgcgttga tgtcctcgtt gatatcaaaa atgagagaga agataaaaag cgtagcgagg
     5701 ctattcgcgc cttagattca tggatgcgag atgggtatag tgctgcgcaa atttatgact
     5761 tagcaatatc aaaaggcgaa ataccaggag tacgcatcga ataagacata accgatattc
     5821 gaattgaaga actgaaagaa caccaagccg cctgatggcg gtttcttttt gcctggagaa
     5881 ttaagatgac cgataccagc ctgattcctg agaaagaagt gatgaacaag ctcggtgttt
     5941 catcacgtca gacaatctgg aactatacca aacgacacgg atttccgaag ccagtcagaa
     6001 cccaccccaa atcatacctt cgtgaagctg ttgaagggtg gattcttaac ggtggcgtta
     6061 atcagaaatg ctcctgatgt gccagaacaa tttatcagcg tgaagctcat acgctttgcg
     6121 ctgagcctct atccaatcgt gtttattgta tacggacatt atcccgccaa gttcatgacc
     6181 gagcattcgc tcagtaacat gaggctcgac attcatctcg gacagacgtg taacaagcgt
     6241 tcttctgaaa tcatgtgttc gccagtaacc tagatccatc cctcccctta ttctatttac
     6301 gaatcggttg gctgcagcga tgctgatcgg tttgtttatg tcttctccag ggaaaagaac
     6361 atcattgtac gttgtcatgg ccttttcgag aaaaggctta atttgttcga agattggacg
     6421 acggataaca ttacccatct tgctatgctc tttcggtacg gtccatacgt tgtccagcag
     6481 gtcaaattct gtctttgttg ccagcctaag ctctgagagc ctcgcccccc acagcataag
     6541 catctgatga aggagcttat ttgacgtaga cgcacggctt ctttcaatag caagccaaat
     6601 cttagccagt tcgtgatacg acagtacccg atcccctacc tcagcgcggg agccgaagtc
     6661 ccttggctgg atgctcataa ttgcgcaact atctatcaac tgacgccgca tgcaccaact
     6721 aattgctgat cttagttgac ttagcacctg ccttgctcgg cgtggattat ctttttcttc
     6781 ttcggtaagc aggtcaaccc attgcttaac cgtgatagaa gatgccggac gattaggaaa
     6841 ggcgtcatgc atgcgcttca taaccgttga tcggtaaagt gcctgggtct tttctctgag
     6901 agttgtagag acgtagttgt cgaaccagta gtcgagacac tgggcgaccg tcatggagtt
     6961 ctccaccttc tcttcaaaat aagtgcgtgg atccgttcct gagaaataga gctttcgcaa
     7021 gtcagcagtg atctgtctgg catccttcaa agacagggat gggtatcgac caagcccaag
     7081 tcgattaggc ttgccatgcc agcgatagcg gtactggaac tggatgaccc ccttcggtga
     7141 aattcgtacg ctgaggccat cggcatcagc cacttcttgt gggcccgaat atggtttacc
     7201 ataaatagta cgcagttttg tgtcgcttat agccataaat aatatttggt acgctcaaaa
     7261 ataatggttt ggtacacatc tggtacacaa tatcacatgg acaacagcgc acagtaaaca
     7321 actatatttg acgttcgtag acatactcag gtgaaaaaat ggttgttttt cgagttataa
     7381 cagacagtta attaactaca gtggacaatg ctagacaatg acagacacag ataaacaacc
     7441 taccttcctc tttcacgatt acgaaacctt tggaacgcac cccgcgttag atcgccctgc
     7501 acagttcgca gccattcgca ctgataacga attcaatgtc atcggcgaac ccgaagtctt
     7561 ttactgcaag cccgcggatg actatttacc ccagcctgga gcagtattaa ttaccggtat
     7621 taccccgcag gaagcacggg cgaaaggaga aaacgaagcc acgtttgccg cccgtattca
     7681 ctcgcttttt accgtaccga agacctgtat tctgggctac aacaatgtgc gtttcgacga
     7741 cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat ccttacgcct ggagctggca
     7801 gcatgataac tcgcgctggg atttactgga tgttatgcgt gcctgttatg ccctgcgccc
     7861 ggaaggaata aactggcctg aaaatgatga cggtctaccg agctttcgcc ttgagcattt
     7921 aaccaaagcg aatggtattg aacatagcaa cgcccacgat gcgatggctg atgtgtacgc
     7981 cactattgcg atggcgaaac tggtaaaaac gcgtcagcca cgcctgtttg attatctctt
     8041 tacccatcgt aataaacaca aactgatggc gttgattgat gttccgcaga tgaaacccct
     8101 ggttcacgtt tccggaatgt ttggggcatg gcgcggcaat accagctggg tggcaccgct
     8161 ggcgtggcat ccagaaaatc gcaatgccgt aattatggtg gatttggcag gagacatttc
     8221 gccattactg gaactggata gcgacacatt gcgcgagcgt ttatataccg caaaaactga
     8281 tcttggcgat aacgccgccg ttccggttaa gctggtgcat atcaataaat gtccggtgct
     8341 ggcccaggcg aatacgctac gcccggaaga tgccgaccga ctgggaatta atcgtcagca
     8401 ttgcctcgat aacctaaaaa ttctgcgtga aaatccgcaa gtgcgcgaaa aagtggtggc
     8461 gatattcgcg gaagccgaac cgtttacgcc ttcagataac gtggatgcac agctttataa
     8521 cggctttttc agtgacgccg atcgcgcagc aatgaaaatt gtgctggaaa ccgagccacg
     8581 taatttaccg gcactggata tcacttttgt tgataaacga attgaaaaac tgttgttcaa
     8641 ttatcgggcg cgcaacttcc cggggacgct ggattatgcc gagcagcaac gctggctgga
     8701 gcaccgccgc caggtattca cacccgagtt tttacaaggc tatgcagaag agattcagat
     8761 gctggcgcag cagtatgccg acgataaaga aaaagtggcg ctgttaaaag cgctttggca
     8821 gtacgcggaa gagatcgtct agtttgaagc ctccgccgct gatacggcgg aggacaatgt
     8881 tgggtcaggc tttttgaacg gtgatgctcc acgcagcatc gccaatctgc tgataatcgg
     8941 tgatggcatg tccttcttct gccgcccatt gtgggattgc ttctgttgcc tgggtgcaat
     9001 caaactcaat taccaactca tcgccgctgg ccatttctgc cagtgctgct tttgcttcta
     9061 tgagtggaaa cggacagacc tgcgtcacca catccagctt tttaatcgcc atctttacac
     9121 cttacttaat ttgccgcagc agttgccagt cgcgccttac gctgcggtcg aacatacaca
     9181 agccaggacg cagtccagac tccgagaatc ataaatacaa ggccaatcca gccctgccag
     9241 gtcatcattg cagtcataac cagtccatta ccgatagagc aaccacctgc aatactggca
     9301 ccgaagccca tcagtacacc acctaacccg ctacgtaatg ttgtttgtgc atcagctgcg
     9361 cgaacgcgga actcacggct cgctttggct gcaataaatg accccacgaa gatccctaac
     9421 accaggaaaa cgccccagtt aatgtatttc acgtcacccg cgaccagaaa ttgcagaata
     9481 ttggccgttg gggaagtgat tcctaaccca aacatgcgtc cggttgcttc actcaggggc
     9541 caggctaaaa gcgcaatcaa accgatgagt acagcagtga caaagggatg ccagcgtttt
     9601 tcaaacagaa tatgagcgat cccggttcgg cgcggcggta aggtcgcgac tttgagtttt
     9661 ggcttcttca actctttcat caccacccag agcgttatca ccagcaaaac ggcaaccaac
     9721 ggccacacag acaaattaaa agtctcagca atagagttat gttcagtact gtagtgctgc
     9781 aaggtttgat ttaaaccact ggcatgtgga gaacgcatca ccgcactcat caccatataa
     9841 gtgaaaagcg cgatccaact gccgatcaat ccctcacccg cgcgatacca ggtcccggtg
     9901 gcacatccac ccgccagaac aattcccagc ccgaagatat acccacctat aacagtacca
     9961 agccacggga acgccccggc ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc
    10021 cccacgcttt gtaccgaaat tgcaatcagc aaggcgtaaa acatgcgatt atttttcacg
    10081 atatacatat cgcgaaaacc acctgtcagg caaaaacgcc cacgctgcat gacaaatccc
    10141 agcagagcac cacaaattag cccgcttaat atcattgaaa acataaatta attaaccaga
    10201 tgaatgttaa tagggaaatt attcatgact ggtggaatgc agaccaataa ccaaattctc
    10261 taaattagaa caaatggtta ttaatgagga tgtcgataaa aaagaataaa aaaaccggag
    10321 cccaatattc attcgactcc ggtttattat tacaaaagat gattacgcta cgtcttcgta
    10381 ctgcggaacc gggttacgga aactcttggt gacgcaagcc aggtaaatca gaccgatagc
    10441 cgcccagatc agacccagaa ccattgagct ttcttccagg ttaacccaca gcgcaccaac
    10501 ggtcagcgca ccacacatcg gcaggaacag atactggaag tgatctttca gcgtcttgtt
    10561 acgcttctca cggatccaga actgcgagat gaccgacagg ttaacgaagg tgaacgccac
    10621 caacgcacca aagttgatca gcgctgtagc cattaccagg tcgaagttga ttgccagcaa
    10681 ggcaatcgcg ccaaccagga tgatgttcat cgccggagta cgccatttcg ggtgtacata
    10741 accgaagaag cttttcggga acacgccgtc acgacccatt acgtacatca gacgcgctac
    10801 gcctgcgtgc gccgccatac cggacgccag tacggtaatg gtggagaaga tcagcgcacc
    10861 aacctggaat gctttacctg caacatacag catgatttca ggctgtgacg catccggatc
    10921 tttaaagcga gagatatccg ggaagtacag ctgcaggaag taagttgcaa agatgaagat
    10981 catgccgcca atcagcgcgg tcaggaaaat cgcacgcggg atcacgcgct ctgcatcttt
    11041 ggtttcttcc gacaggttgc tgatgccgtc aaagccggta aaggagaagc acaggattgt
    11101 cgccccggta atcatcggga taacatgcgc atcgccagac cagaatggac gagtgctcgc
    11161 cagcgtacca gcgccttcac cttcaaatac gccataaaca accatgccca gaatcaccgc
    11221 gatcagcacc acctgcaaca cgacgattac agtgttgaag ttcgctacgg atttcagact
    11281 acgcaggtta aaggcggtca tgaaggccac caacgccacc acaaacatcc acgatgggat
    11341 ggaaggcacc agagcttcaa aatagatttt cgccagcaga atgttgatca tcggcgcgaa
    11401 cagatagtcg agcagagaag accaacccac cataaagccg acagtcgggc taatggattt
    11461 ctgggcgtaa gtgtatgcag agccagcaga aggatagcgg cgaaccagct tcccgtagct
    11521 cagagccgta aacaggatcg caatcaatgc gaacgcatag gctgtcggca catgaccatc
    11581 cgtgaggcct gaaacgatac caaaagtatc aaacagcgtc atcggctgca tataggcaag
    11641 acccatcatt acaaccggaa ctaacgtaag cgttttacgc aattccacgc gagaggtgtt
    11701 tggagtaacg ttatgcgaca tggtcattct cctttacggt gataaccgtc gcgtaagcaa
    11761 aaaattgccc catttttttg gattcctcag cgacaacaac tgtcgatttt tagtaaatat
    11821 ctatccggta cgaagcccgg cctcttggta tgaatgattg gtttgaagca aaaaataacc
    11881 gacgctgatg aaacgtcggt ttttagtcat tttttgacag cggcgcattg tgcctaaatg
    11941 ggggggaaat gacaagagaa tgagaggctt gtcataataa tttttcttta aatggctgat
    12001 tttccgtcat cagattttgc ataaacaccg gcgatcgcac tatttgctaa aatttcatcc
    12061 cgccaccagc cgctggcaag ccctgcggtc tgttcgttcc agccaatcca gacgggttca
    12121 aagagagttt gggcgaccac tttcttctcg attaacgcgc cactatcgag aaaacgttgc
    12181 gccagataac ggggtaaata gccacagccg aggccgctaa tttgcagctc cagcttggtt
    12241 ttaaaatcga aaacggtaat ggcttcttgt tcgtcgagaa gttgtgaggc cgtagaagcg
    12301 cctgcctgcg cggtatcccc caccacaatc gcccgatatc gcttaataat acgacgattt
    12361 aacggctctt cttccatggc taacggatga tgaggcgcga ccgcaaagac ttgttccaga
    12421 tcgcccaacc gcgagaaacc aaactcactg gaagatggtg gctcatgcat cgcgccaaca
    12481 ataatatccg ctcgcccctg agtcagggca tcccaggaac caccgagtac gccattgata
    12541 aacttcaggc gcgtcacgct atgatgttga taaaaagctt caatcagtgg cgcaagcaga
    12601 gaaaaaggaa acgtgtcgtc cacgccaatc accagctcgt tttcccagtc ttcatgcagt
    12661 ttgatcgcct gcttttccag ttctcgcacg gtatgcagaa cttcccgccc cttctctaat
    12721 aacatttttc cggtacgggt gaatttagcg cggtgaccgc tacgatcaag caattgaata
    12781 ttaaggtcgc tttccagctt gtgaacggtg taactcagag cggacggcgt cttgtagagt
    12841 ttggccgacg ccgccgcaaa actgccttct ttttctaagg catcgaggat catcaacaca
    12901 tccagcagtg gtttcataca cgcccccttg cagtgcatgc ggcgtcctgc tggcgtgatc
    12961 gtttactcca gcggcattac cagcggatcg ggatactggt attcaaaccc cagttcatta
    13021 caaatccgac tgccatcaat aatcttacct ttgccgctgt ccagactatt tctgaactgc
    13081 ggaggttcca gccccagtaa acgggccatc tgcggataga aaacattacg cgcagggtga
    13141 gctggcgcac atatattata gatgtgtccg cctttaggtg cctgtaacaa cagagtgata
    13201 gcgccaataa catcttctaa atggactaaa ttaacaccat gttcaccatc aggcgcggtt
    13261 tttccggcaa agaagcgtcc gggatgacgt cccggtccga ccaggcccgc aagacgcaga
    13321 atatcgaccg aagtaccggg taaattgtgc agccagtctt cgagttcttc taatactcgt
    13381 ccactgttgg ttaccggatt acgcgaggtg gtttctttca ccgtgccttg cgcgtcgcca
    13441 tagacagatg ttgagctggt aaaaataatt cggggaatac gatgggccag cgcgctatcc
    13501 actaactctt gtaccgcttg taaatagaac tcatcgccgg agccgctacg acgtgccgga
    13561 agcgtaatga ccagtgcatc ggcatccatc agggcatcca gatcgtcaga atcgcaaact
    13621 aactcaggct ccatgcgaag cagatagcta tcaatgccac tcattcgggc cgcttcgaca
    13681 ccatcttgtg tggttttact cccggtgact tgccagcctc gcgctgaaag tgacatcgcc
    13741 agcggcatgc ctaaccaccc taacccgaca attgcgacct ttttcatccg ttatctcctg
    13801 atacacctta gatctataag gctacgctag cgtatcaaaa ctgacaattc attctatgaa
    13861 tgaatctgtt caataatgat aacgacatgc tgcaatgagc agtgaatcgt cagtaaccgc
    13921 gtataccaga cggtgctcct ctgtaatgcg tcgggaccag aagcctgaca aattatgctt
    13981 caggggttct ggcttcccct taccttcaaa tggtgttctg cgggtatctt tgataagttc
    14041 attgatcttt ttaacaattc gcttatctgt ttcctgccag tacagataat catcccatga
    14101 ctcctcagac cagattagtt tcactcaata atgtcctttt ccgttccttt gcctgatttc
    14161 aggctatcga ttgagtccat caatctccgg gcgttagcgg gggaacgcag tagataagcc
    14221 gtctcttcca gcgagttgta ttcttcgagt gacatcagaa cacaagcctc tccattctga
    14281 cgagtaataa ggatcggggc atgatcttca acggctttca tcattgttgc cgacaaattc
    14341 tgacgcgctt cgctgtaact aattgtacgc atgtcaatct cctcttttgt acagcttatt
    14401 gtacaatgat agacgttgat tcactattta ttaattagtt tgtagatcaa gacattgtca
    14461 gtgagacgaa aaatccaggc ttcgctattt tgggtaccgt caggttagag gacagtcctc
    14521 ttagcgccct cctttccccg ctcattcatt aaacaaatcc atcgccataa aatatataaa
    14581 aaagcccttg ctttctaacg tgaaagtggt ttaggttaaa agacatcagt tgaataaaca
    14641 ttcacagaga cttttatgac acgcgttcaa tttaaacacc accatcatca ccatcatcct
    14701 gactagtctt tcaggcgatg tgtgctggaa gacattcaga tcttccagtg gtgcatgaac
    14761 gcatgagaaa gcccccggaa gatcaccttc cgggggcttt tttattgcgc ggttgataac
    14821 ggttcagaca ggtttaaaga ggaataagaa aatgacagac aacactcgtt tacgcatagc
    14881 tatgcagaaa tccggccgtt taagtgatga ctcacgcgaa ttactggcgc gctgtggcat
    14941 taaaatcaat cttcacaccc agcgcctgat cgcgatggca gaaaacatgc cgattgatat
    15001 tctgcgcgtg cgtgacgacg acattcccgg tctggtaatg gacggcgtgg tagatcttgg
    15061 gattatcggc gaaaacgtgc tggaagaaga gctgcttaac cgccgcgccc agggtgaaga
    15121 tccacgctac tttaccctgc gtcgtctgga tttcggcggc tgccgtcttt cgctggcaac
    15181 gccggttgat gaagcctggg acggcccgct ctccttaaac ggtaaacgta tcgccacctc
    15241 ttatcctcac ctgctcaagc gttatctcga ccagaaaggc atctctttta aatcctgctt
    15301 actgaacggt tctgttgaag tcgccccgcg tgccggactg gcggatgcga tttgcgatct
    15361 ggtttccacc ggtgccacgc tggaagcaaa cggcctgcgc gaagtcgaag ttatctaccg
    15421 ctcgaaagct tgcctgatcc agcgcgatgg cgaaatggaa gaatccaaac agcagctgat
    15481 cgacaaactg ctgacccgta ttcagggtgt gatccaggcg cgcgaatcaa aatacatcat
    15541 gatgcacgca ccgaccgaac gtctggatga agtcatcgcc ctgctgccag gtgccgaacg
    15601 cccaaccatt ctgccgctgg cgggtgacca acaacgcgta gcgatgcaca tggtcagtag
    15661 cgaaaccctg ttctgggaaa cgatggaaaa actgaaagcg ctgggtgcca gttcaatcct
    15721 ggtcctgccg attgagaaga tgatggagtg atcgccatga gctttaacac aatcattgac
    15781 tggaatagct gtactgcgga gcaacaacgc cagctgttaa tgcgcccggc gatctccgct
    15841 tctgaaaaca ttacccgcac tgttaacgat attctcgata acgtgaaagc acgcggcgat
    15901 gaggccctgc gggaatacag cgcgaagttt gataaaacca cggttaccgc actgaaggtg
    15961 tctgctgagg aaattgccgc cgctagcgaa cgcctgagcg acgagctaaa acaggcgatg
    16021 gcggtggcag taaagaatat tgaaaccttc cacactgcgc aaaaactgcc gccggtagat
    16081 gtagaaacgc agccaggcgt gcgttgccag caggtcacgc gcccagtcgc ttcagttggg
    16141 ttgtatattc ctggcggctc cgccccgctc ttctcaacgg tattaatgct ggcgactccg
    16201 gcgcgtattg cgggctgtaa aaaagtggtg ctgtgctcac cgccgccgat tgccgatgag
    16261 atcctttacg cggcgcagct gtgcggcgtg caggacgtgt ttaacgtcgg cggcgcacag
    16321 gccattgccg cgctggcgtt tggtactgaa tctgtgccga aagtggacaa aatcttcggg
    16381 ccgggtaacg cctttgtcac cgaagcaaaa cgccaggtaa gccagcgtct ggacggtgcg
    16441 gcgatcgata tgcccgcagg cccgtcggaa gtgctggtga ttgctgacag cggcgctacg
    16501 ccggatttcg tggcttctga tttgctttct caggctgaac acggcccgga ctcacaggtg
    16561 attttactga cgcccgatgc tgatatggcg catcaagttg ccgaggccgt cgaacgccaa
    16621 ctggcagaac tgccgcgtgc cgaaaccgcc cgtcaggcac tgaacgccag ccgcctgatc
    16681 gtgactaaag atttagcgca gtgcgtagag atctccaacc agtacggccc ggagcacctg
    16741 atcatccaga cccgcaacgc ccgcgatctg gtcgatggca tcaccagcgc cggttcggta
    16801 tttcttggtg actggtcacc ggaatccgca ggcgactatg cttctggcac caaccacgtt
    16861 cttccgacct acggctatac cgcgacctgc tcaagccttg ggctggctga tttccagaaa
    16921 cggatgaccg tacaggaatt aaccccacaa ggtttctctg ccctggcctc aaccattgaa
    16981 acactggccg ctgctgaacg actgactgcc cacaaaaatg ctgtcaccct gcgcgttaac
    17041 gccctgaagg agcaagcatg agcactgaca actttctcag cgtcactgat ttagcccgta
    17101 agaatgttcg cgagttaaca ccctatcagt ctgcccgccg tctcggcggt aacggcgatg
    17161 tctggctcaa cgcgaatgag tacccaaccg ccgttgagtt ccagctcact cagcaaacgc
    17221 tgaaccgcta ccctgaatgc cagccgaaag cagtgattga gaattacgcg cagtatgctg
    17281 acgtcaaacc ggagcaggta ctggtcagcc gcggcgcaga tgaagggatc gaactgctgg
    17341 tgcgcgcttt ttgcgaaccc ggtaaagatg ccattctcta ctgcccgccg acttacggca
    17401 tgtacagcgt cagcgccgaa acgattggcg ttgagtgccg caccgttccg acccttgata
    17461 actggcaact ggatttgcaa ggcatcgcgg acaagcttga tggcgtgaag gtggtctacg
    17521 tatgcagccc taataaccca accggccagc taattaaccc gcaggatctg cgttcactgc
    17581 tcgacatgac gcagggtaaa gctatcgtgg ttgccgatga agcgtatatc gagttttgcc
    17641 cgcaggccac gctggcaggc tggctgtctg agtatccgca tctggtgata ttacgcacgt
    17701 tgtccaaagc gtttgcgctc gccggtttac gctgcgggtt tacgctggcg aatgaagagg
    17761 ttatcaatct gctgttaaag gtgattgccc cttacccact ctctacgccg gtggcagata
    17821 tcgccgccca ggcgctgacg ccgcaaggga tttccgccat gcgtaaacgc gtagagcaga
    17881 tcctggaaga gcgtcagtat ctggtagata acctgaaaac aattcgctgt gtagagcagg
    17941 tattcgattc tgaaaccaac tatgttctgg cgcgcttcac cgcatcaagt agcgtattta
    18001 aatctttgtg ggatcagggc attatcttac gagatcagaa taaacaacct tcgttaagcg
    18061 gctgcctgcg aattactgtc ggaacccgtg aagagagcca gcgcgtcact gacgccttac
    18121 gtgcggagca agtatgagcc agaagtatct ttttattgac cgtgatggaa ccctgatttc
    18181 cgaaccgcca agtgactttc aggtcgatcg ctttgacaaa ctcgccttcg agccggaagt
    18241 tgttcccgtt ttgctgaaac tgcaaaaagc aggttttaag ctggttatga tcactaacca
    18301 ggatggactc ggaacccaga gtttcccgca ggctgatttt gacggtccgc ataacctgat
    18361 gatgcaaatt ttcacctctc agggcgtaac gtttgatgag gtattgatct gcccgcactt
    18421 gccagcggac gagtgtgatt gccgtaagcc aaaagtgaag ctggttgaac agtatctggt
    18481 tgaacaggcc atggatatgg cgaacagcta cgtcattggt gatcgggcga ccgatattca
    18541 gctcgctgaa aacatgggca ttagcggatt gcgttaccat cgcgataccc tgaactgggc
    18601 gatgatcggc gagcagttga ccaaacgtga ccgctacgca cacgttgagc gtaatactaa
    18661 agaaacgcag attgacgtca aagtctggct ggaccgcgaa ggccgcagca aaatcaatac
    18721 tggcgttggc ttctttgacc acatgctgga tcaaatcgcc acccacggcg gttttcgcat
    18781 ggagattgcc gtcaagggcg atctgtatat agacgatcac cacaccgttg aagataccgg
    18841 tctggcgctg ggcgaagcct taaagctggc gttaggcgat aaacgcggca tcaaccgttt
    18901 tggttttgtg ctgccgatgg atgagtgtct ggcacgttgc gcgctggata tctccggccg
    18961 tccacacctg gagtaccgcg cggagtttac ctaccagcgc gttggcgatc tgagcacgga
    19021 gatgatcgaa cacttcttcc gttcactctc ttacactatg ggcgtcaccc tgcacctgaa
    19081 aaccaaaggc aaaaatgacc accaccgtgt ggagagcctg tttaaagcct ttggccgtac
    19141 gctgcgtcag gcgattcgtg tagaaggcga cacgctgccc tcttcaaaag gagtgctgta
    19201 atgaacgtgg tgattctgga caccggctgc gccaacctgc actcggtaaa atcagccgtc
    19261 gcacgccacg gatatgatcc tgttgtcagc cgtgacccgg acgtggtgct gcgcgccgat
    19321 aaactgtttc tgccgggcgt cggcaccgcg caggcggcta tggaccagtt gcacgagcgt
    19381 gagctgattg aactgattaa agcctgtacg caaccggtac tggggatctg tttaggtatg
    19441 caactgctgg gccgtcgcag cgaagagagt aacggtgtct ccctgctggg cattattgag
    19501 caggacgtcc ctaagatgac cgatttcggt ctgccgctgc cgcatatggg ctggaatcgc
    19561 gtctatccgc aggcgggaaa tcgtctgttc cagggcattg aagatggcgc ctatttctac
    19621 tttgtacaca gctacgctat gccggtaaac ccgtggacca tcgcccagtg caattacggc
    19681 gaaccgttta ccgcggcagt acaaaaagac aatttctttg gcgtacagtt tcacccggaa
    19741 cgttcgggcg cggctggggc acagttgctg aaaaactttc tggagatgta atgattattc
    19801 cggcattaga tttaatcgac ggcacggtgg tgcgtcttca tcagggcaat tacgctaagc
    19861 aacgtgatta cggcaacgat cctctccccc gtttgcagga ttatgcagcc cagggtgctg
    19921 aagtgctgca cctggttgat ttaaccggcg cgaaagaccc ggcgcagcgc cagatcccgc
    19981 agatcaaaac gctggtcgct ggcgtcaatg ttccggtaca ggttggcggt ggcgtgcgta
    20041 cggaagcaga cgtggcggcg ctacttgacg caggtgtggc gcgcgtggtg gtgggttcga
    20101 cggcggtaaa atcccccgag atagtcaaga gctggtttgc ccgttttggt gccgatgcgc
    20161 tggtactggc gctggacgtt cgtattgacg agcacggtaa taaacaggtt gcggtcagtg
    20221 gctggcagga agattccggg gtttcactgg aagaactggt tgagacctat ttacccgtcg
    20281 gcctgaagca cgtgctgtgc accgatatct cccgcgacgg tacgctggca ggctcaaacg
    20341 tcgcgctgta tgaagaagta tgcgccaaat acccgcaggt ggcgtttcag tcatctggcg
    20401 gcattggcgg aatcgaggac gtagcggccc tgcgcgggac cggtgttcgt ggtgtgatcg
    20461 tcggacgagc cctgctggaa ggtaagttca ccgttgagga ggccattcaa tgctggcaaa
    20521 acgcataatt ccttgtctgg acgtccgtga tgggcaggtg gtaaaaggtg tccagttccg
    20581 caatcacgag attattggtg acatcgtccc gctggcaaaa cgctatgccg atgaaggtgc
    20641 agatgaactg gtgttctacg atatcaccgc ttccagcgat ggccgcgtag tggataaaag
    20701 ctgggtatca cgcgtggcgg aagtgattga tattccgttc tgcgtagccg gcggcattaa
    20761 atccatcgac gatgcggcgc gcattctctc ctttggggcg gataaaatct ccatcaactc
    20821 cccggcgctg gccgaaccgg aacttatcac ccgtctggcg gatcgctttg gcgtgcagtg
    20881 catcgtggtg ggcattgata cctggtatga cgccgacacc ggtaaatatc atgtaaatca
    20941 gtatacggga gatgaaagcc gtacccgcgt cacgcagtgg gaaacactgg actgggtgca
    21001 ggaggtccaa aaacgcggcg cgggcgaaat cgtcctgaac atgatgaacc aggacggcgt
    21061 acgtaacggt tatgacctgg aacaactgaa aaaagtgcgt gacgtttgcc acgtaccgct
    21121 gattgcctcc ggcggtgcgg gcaccatgga acacttcctc gaagccttcc gggatgccga
    21181 cgttgacggc gcgcttgccg cctccgtctt ccacaaacaa attatcaata ttggcgaatt
    21241 aaaagcgtac ctggcaacac agggcgtgga gatcaggata tgttaacgaa gcaacaatgc
    21301 cgcgagctgg actgggaaaa aaccgacgga ctgatgccag tcatcgtgca gcatgcggtt
    21361 tccggtgaag tattgatgct ggggtatatg aacccggagg cgctggataa aacgctcgaa
    21421 agcggcaaag tgaccttttt ctcacggacc aagcagcgcc tgtggaccaa aggagaaacc
    21481 tccggtcact tcctgaatgt ggtgagcatt gcgcctgact gcgacaacga caccctgctg
    21541 gtgctggtta acccgattgg cccaacctgc cataaaggca cctcaagctg ctttggtgac
    21601 gccagccacc agtggctgtt cctgtatcag cttgagcaac tgctggccga acgtaaaacc
    21661 gctgacccag ccagctccta caccgcgaaa ctgtatgcca gcggcaccaa acgcatcgcg
    21721 cagaaagtgg gcgaagaagg cgtagaaacc gcgctggctg cgaccgtgaa cgaccgtttt
    21781 gagctgacaa atgaagcctc tgatctgatg tatcacctgc tggtgctgct gcaagaccag
    21841 gacctcgacc tgacgacggt gattgagaat ttacgtaagc gtcatcagta agttgcagac
    21901 gtaaaaaaaa ccgggcgctg cccggttttt tgttatcagc tttagactta ctcagctttg
    21961 gctttgtaac cgcgcagagc atttcgccca agcaccacac cggcaccaat catcccaccg
    22021 atcagaactg ccagcacgag ggtaatcgct tttttcgggc tgtcacgacg aatcggtaac
    22081 gttggtttca ttacataacg gtaggcgtgc acgctgtcag gtgccacttc taatgcctgc
    22141 acctccaaca gattctgacg cgtttggtag tactggtcag aaaacgtcaa aggacgtgta
    22201 gcttcgttct caaccattga ggaaagagcc tcactaccca gcataaacat agaatcctga
    22261 gatacttgat caacctgctg tacctgcggt agcttaatat tagactgttg cgcaacctta
    22321 agtgcttgag tcatctgcgc aatacgcagg gttttctgct ctttcgccac tgcctcctgc
    22381 gctgcaagag atttttgcaa atcagccagt cttgtttcca tactcattga caagtcagca
    22441 ttgagctctt tagcaacacc ttcatcaact tgttgaatat actgagccag cgttttttgt
    22501 gcctcttcag aggttttgcc ggtataagaa acctttaacg gcagagtctg acctttaaca
    22561 gcagcatcaa ttgaaagttt ttccggttct tgctggttat ccaacgtttc agccagtgca
    22621 gaaaatgccg aactaaaacg cccgataaaa cccgcctgaa catctgtcat ttcgggtgcg
    22681 ttagccccaa acagtacatc catagcatta ttgtagctgg caagctggcc agcatcgggt
    22741 tgtgtaacga tagctatcga ggtccatttc tccttagcca ccgccaaata gcctactgcc
    22801 aacgctattg ccacaacaat gcagataata atcgttacct tcccacgcca taactgcatg
    22861 agtaaatcaa tcaaatcaat ctgttccgga tcgtgactgc gaccggacat gttgttatca
    22921 cttatagaca tagaaaccct agataaggaa agacttaaag ggggatcagt ttagctataa
    22981 attgaggcaa tgccatagga ttaatgataa aaaaatcaaa ttgtttgggg aattttgatt
    23041 ggtatttcaa tcactactgg ggaacgatac gattaaacaa aagtgtcaat atctaaagaa
    23101 cactttcatg cgcaaaaaac catcaacaaa caggtttatg cccgccaatc gttacacttt
    23161 acaattcatt ttattgaatt catatcgtga atacttaccg tctgaccagt aaaaatggta
    23221 agcgtaaaaa aatataacaa ttagcttttt taccatctaa cggtatcatt ccacttttaa
    23281 tagcacctgt tggtgcctgg ctactatttg gccgaaaaga gggataaaca tgaaatacct
    23341 ggttacgggt gccgctggtt tcatcggctt ccacgttagc aaacgcctcc ttgaggctgg
    23401 ccatcaagta gtcggtattg ataatctgaa cgattactac gacgtcagcc ttaaacaagc
    23461 tcgtctggaa ttactggcac aacctggttt ccagtttcat aaaattgatt tagccgaccg
    23521 cgaaggcatg accgatctct tcgcatccgg gcatttcgaa cgtgtgattc atctcgccgc
    23581 ataggctggc gtacgctatt cactcgaaaa cccgcatgcc tatgctgact caaatctcac
    23641 aggatttctg aacatactgg aagggtgtcg ccataataaa attcagcatt tactctatgc
    23701 ttcctccagt tctgtttacg gcctgaatcg caaaatgcct ttctctacgg atgattccgt
    23761 tgatcacccc gtatctctgt atgcggcaac caaaaaagcc aacgaactga tggcccatac
    23821 ttattcccat ttgtacggat tgcccgcaac cgggttgcgt ttctttaccg tgtacggccc
    23881 ctggggtcgt ccggatatgg cactgtttaa gttcaccaaa gcaatgctgg aagggaaaag
    23941 tattgacgtc tataactacg gcaaaatgaa gcgtgacttt acctatatcg atgatatcgc
    24001 agaagcaatc attcgcctgc aggatgtcat tccacacgca gacacacaat ggaccgttga
    24061 aacaggcacg ccagcagcca gcattgcccc ctatcgcgtc tacaatatcg gcaatagttc
    24121 accggttgag ctcatggact atatccaggc tttggaagat gcgctgggta ttgaagcaaa
    24181 gaaaaacatg ctaccgctac agcctggtga tgtgctggaa accagcgcgg acactaaagc
    24241 actgtatgag gtaattggtt ttacgcctga aaccacggtc aaagatgggg tgaagaactt
    24301 tgttaactgg taccgtgatt tctacaaagt ttgattgctg atctgattaa aataaaaagg
    24361 ccttttataa ggcctttttt atggaaacga gattaatcac taccaaacaa accacgggta
    24421 tacactttat cggcaacatc agctaattct gacgtcatgc ggttagaaat aataatatct
    24481 gcttcatttt tgaacgcagt aagatcacgc actacccgag aatggaaaaa ctcatcctct
    24541 accatgacgg gttcataaat aataacaggt acccccttgg ctttaatacg cttcataatg
    24601 ccctgaatag atgatgcccg gaaattatcg gaaccacttt tcatgatcaa acggtacacg
    24661 ccgaccacct taggcttacg cgcaagaata gaatctgcaa tgaagtcctt acgcgtacgg
    24721 ttcgcatcaa cgatcgcgcc aataatgtta ttcggtacag acgcgtagtt agccagcaac
    24781 tgcttagtat ctttcggcag acagtagcca ccataaccga atgacgggtt gttgtagtga
    24841 ttaccgatac gcggatccag gcacacacct tcaataatct gccgtgagtt caagccaaga
    24901 ctttctgcat agctatccag ctcgttaaag taggccacac gtaacgccag ataggtgtta
    24961 gcgaacagct tgatggcttc ggcttcagtc gagtcagtaa acagcgtcgg aatgtctttt
    25021 ttaaccgcac cttcctgcag caacgcggca aaacgcccgg ctcgttcgga acgttcacca
    25081 ataacgatac gggacggatg caagttgtcg tacagtgcac gaccttcacg caggaattct
    25141 ggcgaaaaga atatattgtc gatacctaac cgttctttaa gtaagtttgt aaagccaacc
    25201 ggaattgttg atttaattac catcaccgca tttgggttga tgtcgaccac atcgcggatc
    25261 actgactcga cgctcgaggt gttaaagtag ttagttttcg gatcgtaatc agtcggtgtc
    25321 gcgatgataa cgtagtccgc gtcgcggtaa gcatcatact tatcggtcgt tgcacggaaa
    25381 ttcagctgtt ttgtcgccag atattcttcg atttccttat caacgatcgg cgattttttc
    25441 tgattcagca tctcgacctt cgttggaacg atatccagag cgaccacttc gtggtgctgg
    25501 gcaatcagaa taccgttcga caaaccaaca taacctgttc ctgagatagt tatcttcatt
    25561 cgctgtgtaa cttcttccgg ttgaacattt gagggtgaac acgctgccaa caacgtggca
    25621 catcggctct gcattgcatc agtatatcgt gatttgctac atcaccggat atcggacctg
    25681 actgaatccc attttttggc aataaaaaag cccggaggag cgctccgggc ctggatttac
    25741 agttagacta aatcagatta atccagccat tcggtgtgga acacaccttc tttatcagtg
    25801 cgtttatacg tgtgcgcacc gaagtaatca cgctgtgcct gaatcagatt agccggcagt
    25861 accgcagaac ggtagctgtc gtagtaggct accgctgcag agaaggtcgg taccggaata
    25921 ccgttctgca cagcataagc cactacatca cgcagcgctt gctgatattc atcagcgata
    25981 tttttgaagt acggagccag caacaggtta gcaatgcctt tgttttcagc atacgcgtca
    26041 gtaattttct gcaggaactg cgcacgaatg atgcagcccg cgcggaagat cttcgcgatt
    26101 tcgccgtagt tcagatccca gttgtattcg tcagacgcgg cacgcagttg agagaagcct
    26161 tgggcataag agacgatttt acccaggtac agcgcgcgac ggactttctc aacgaactct
    26221 gctttatcac cagccagttt agcctgcgga ccagacagca ctttagatgc cgcaatgcgc
    26281 tggtctttca gagaagagat gtagcgagcg aatacggatt cggtgatcag cgacagcggt
    26341 tcacccagat ccagagagct ctggctggtc catttaccgg tgcctttgtt cgcagcttcg
    26401 tccaggatca catcaaccag gtatttaccc tcttcatctt ttttggtgaa gatgtctttg
    26461 gtgatgtcaa tcaggtagct acttagctcg ccttcattcc actcggtaaa agtggttgcc
    26521 agctcttcgt tagacagatt aaggccgcct ttaagcagag aataggcttc agcaatcagc
    26581 tgcatatcgc catattcgat accgttgtgc accatcttca cgtagtgacc cgcaccgtca
    26641 gcaccgatgt aagttataca tggttcgcca tcttcagcaa ccgcagcaat cttggtcagg
    26701 ataggcgcaa ccagctcata cgcttctttc tggccacctg gcatgataga tgggcctttc
    26761 agggcgccct cttcaccgcc ggacacgccg gtaccgatga agttaaagcc ttccgcggac
    26821 agttcacggt tacgacggat agtgtcctgg aagaaggtgt tgccaccatc aataatgatg
    26881 tcgcctttat ccagatacgg cttcagggaa tcgatagcag catcagttcc cgcccctgct
    26941 tttaccatta acaggatacg acgtggggtt tcaagagact cgacgaactc tttcaccgtg
    27001 taataaggaa ccagtttctt atccgggttc tcggcaacaa cttcttcagt tttctcgcgg
    27061 gagcggttga agatggagac ggtataaccg cggctttcga tgttgagcgc caggttgcgc
    27121 cccatcactg ccataccgac gacgccgatc tgttgcttgg acattacata ctcctgtcag
    27181 gtgtagtcac cgcgcgacgc atgcgcggct tgaatgtagt ctagatgaag tt
//
LOCUS       DAZGTR010000057        27087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGTR010000057 DAZGTR010000000
VERSION     DAZGTR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.057"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            86..1684
                     /gene="rtcR"
                     /locus_tag="ACHLCY_002631"
     CDS             86..1684
                     /gene="rtcR"
                     /locus_tag="ACHLCY_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP0369863.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYTRINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1666..2424)
                     /locus_tag="ACHLCY_002632"
     CDS             complement(1666..2424)
                     /locus_tag="ACHLCY_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP0369864.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2441..3271)
                     /gene="glpG"
                     /locus_tag="ACHLCY_002633"
     CDS             complement(2441..3271)
                     /gene="glpG"
                     /locus_tag="ACHLCY_002633"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP0369865.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3316..3642)
                     /gene="glpE"
                     /locus_tag="ACHLCY_002634"
     CDS             complement(3316..3642)
                     /gene="glpE"
                     /locus_tag="ACHLCY_002634"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP0369866.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3832..5337
                     /gene="glpD"
                     /locus_tag="ACHLCY_002635"
     CDS             3832..5337
                     /gene="glpD"
                     /locus_tag="ACHLCY_002635"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP0369867.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5389..5994)
                     /locus_tag="ACHLCY_002636"
     CDS             complement(5389..5994)
                     /locus_tag="ACHLCY_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369868.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     AWGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5997..6764)
                     /locus_tag="ACHLCY_002637"
     CDS             complement(5997..6764)
                     /locus_tag="ACHLCY_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369869.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6767..7477)
                     /locus_tag="ACHLCY_002638"
     CDS             complement(6767..7477)
                     /locus_tag="ACHLCY_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369870.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANYKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7493..9001)
                     /locus_tag="ACHLCY_002639"
     CDS             complement(7493..9001)
                     /locus_tag="ACHLCY_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0369871.1"
                     /translation="MMQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVC
                     NILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLIL
                     VGWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSL
                     GYGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLE
                     SSRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRLISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(9127..11574)
                     /gene="glgP"
                     /locus_tag="ACHLCY_002640"
     CDS             complement(9127..11574)
                     /gene="glgP"
                     /locus_tag="ACHLCY_002640"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP0369872.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11593..13026)
                     /gene="glgA"
                     /locus_tag="ACHLCY_002641"
     CDS             complement(11593..13026)
                     /gene="glgA"
                     /locus_tag="ACHLCY_002641"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP0369873.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13026..14321)
                     /gene="glgC"
                     /locus_tag="ACHLCY_002642"
     CDS             complement(13026..14321)
                     /gene="glgC"
                     /locus_tag="ACHLCY_002642"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP0369874.1"
                     /translation="MVSLEKSDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14339..16312)
                     /gene="glgX"
                     /locus_tag="ACHLCY_002643"
     CDS             complement(14339..16312)
                     /gene="glgX"
                     /locus_tag="ACHLCY_002643"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP0369875.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYVWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16309..18495)
                     /gene="glgB"
                     /locus_tag="ACHLCY_002644"
     CDS             complement(16309..18495)
                     /gene="glgB"
                     /locus_tag="ACHLCY_002644"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP0369876.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18768..19871)
                     /gene="asd"
                     /locus_tag="ACHLCY_002645"
     CDS             complement(18768..19871)
                     /gene="asd"
                     /locus_tag="ACHLCY_002645"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0369877.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20063..20656
                     /gene="yhgN"
                     /locus_tag="ACHLCY_002646"
     CDS             20063..20656
                     /gene="yhgN"
                     /locus_tag="ACHLCY_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP0369878.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20713..22053)
                     /gene="gntU"
                     /locus_tag="ACHLCY_002647"
     CDS             complement(20713..22053)
                     /gene="gntU"
                     /locus_tag="ACHLCY_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0369879.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPVLGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22057..22584)
                     /gene="gntK"
                     /locus_tag="ACHLCY_002648"
     CDS             complement(22057..22584)
                     /gene="gntK"
                     /locus_tag="ACHLCY_002648"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0369880.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22723..23718)
                     /gene="gntR"
                     /locus_tag="ACHLCY_002649"
     CDS             complement(22723..23718)
                     /gene="gntR"
                     /locus_tag="ACHLCY_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP0369881.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23942..24637)
                     /gene="yhhW"
                     /locus_tag="ACHLCY_002650"
     CDS             complement(23942..24637)
                     /gene="yhhW"
                     /locus_tag="ACHLCY_002650"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP0369882.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24760..25797)
                     /gene="yhhX"
                     /locus_tag="ACHLCY_002651"
     CDS             complement(24760..25797)
                     /gene="yhhX"
                     /locus_tag="ACHLCY_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0369883.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            26129..26617
                     /gene="yhhY"
                     /locus_tag="ACHLCY_002652"
     CDS             26129..26617
                     /gene="yhhY"
                     /locus_tag="ACHLCY_002652"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0369884.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            26854..>27087
                     /gene="tssD"
                     /locus_tag="ACHLCY_002653"
     CDS             26854..>27087
                     /gene="tssD"
                     /locus_tag="ACHLCY_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFP0369885.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSIN"
BASE COUNT         6796 a         7210 c         6722 g         6359 t
ORIGIN      
        1 ttaaatcttt acgtccgtaa acggagattt tccacaaaac caattcaccg ataatgaaat
       61 atcgtctttt ataaggatat ctaagatgcg taaaacagtg gcttttggct ttgtcggtac
      121 cgtgctggat tatgccggac gcggcagtca gcgctggtcg aaatggcgtc cgacgctttg
      181 tttatgccag caagaatcgt tagtcatcga tcggctggaa ttgttgcacg acacccgctc
      241 gcgctcgcta ttcgaaacgc ttaaacgcga tatcgccagc gtttcgccag aaacagaagt
      301 cgtgggcgtt gagattgaac tgcataaccc gtgggatttc gaagaggttt acgcctgcct
      361 gcatgatttc gcccgtggtt acgagtttca gccagaaaaa gaagactatt taattcacat
      421 caccaccggc acccacgtcg cgcagatttg ctggtttctg ctggcggaag cgcgttacct
      481 gcccgcccgg ctgatacaat cttcacctcc gcgcaaaaaa gaacagccgc gcggcgcagg
      541 tgaagtgacg attatcgatc tcgatttaag ccgttataac gccatcgcca gccgctttgc
      601 cgaggaacgc cagcaaacgc ttgattttct taagtccggc attgccacgc gtaaccccca
      661 cttcaaccgc atgattgagc agatcgaaaa agtggcgatc aaatcccgcg cgccgattct
      721 gcttaacggt ccaaccggcg cgggcaagtc atttctggcg cgacgcatct tcgagttaaa
      781 acaggcgcgg catcagttta gcggcgcgtt tgtggaagtg aactgcgcca ccctgcgcgg
      841 cgataccgcc atgtcgacgc tgtttggtca tgtaaaaggc gcgtttaccg gggcgcggga
      901 atctcgtgaa ggtttattac gcagcgccaa cggcggaatg ttgtttcttg atgagattgg
      961 cgaactgggc gcagacgaac aggcaatgct gctaaaagcc attgaagaga aaacctttta
     1021 cccgtttggc agcgatcgcc aggtgagcag tgattttcag cttatcgccg gaacggtgcg
     1081 cgatttgcgc cagctggttg ccgaaggcaa atttcgcgaa gatctgtaca cgcggatcaa
     1141 tctctggacc ttcaccctgc cgggtctacg ccagcgccag gaagatattg aaccgaacct
     1201 ggattatgaa gtggagcgcc acgcctcact cactggcgac agcgtgcgtt ttaacaccga
     1261 agcgcggcgc gcctggttgg cttttgcgac ctctccccag gcaacatggc gcggtaactt
     1321 tcgcgaactt tctgccagcg tcacgcggat ggccaccttt gccactagcg gacgcatcac
     1381 tctggacgtg gttgaagatg agataaaccg tctgcgctat aactggcagg agagccgccc
     1441 ctccgcgctt acggcgttgc tgggcgcaga ggcagaaaac atcgatctct tcgaccgtat
     1501 gcaactggaa cacgttatcg ctatctgccg ccaggcaaag tcgctttccg cagccggacg
     1561 ccagcttttt gacgtttcgc gccagggcaa agccagcgtc aatgacgcgg atcggctacg
     1621 caaatacctg gcgcgttttg gtctgacgtg ggaagccgtg caggatcagc acagctccag
     1681 ttgaatatgg tggtccgtca gcacctgcat cacgcttact ggcggcgggg cgtcggtgta
     1741 gacggcatct accatgctga tgctgcccat attgaccatc gcgttacggc caaatttcga
     1801 gtgatcgaca accagcataa cgtggcgcga gttctcaata atggcgcgtt tggtgcgaac
     1861 ttcgtggtaa tcgaactcca gcagcgagcc gtcgctatcg atgccgctta tccccagaat
     1921 gccgaaatca aggcggaact gggagataaa atcgagcgtc gcttcgccaa tgatcccgcc
     1981 atcgcggctg cgtaattcgc caccggcgag aataatgcgg aaatcttctt ttaccatcaa
     2041 cgtgttagca acattgagat tgttggtgac aatacgcaaa ttgctgtgat taagcagcgc
     2101 gtgcgccacc gcttccggcg tggtgccgat atcgataaac agtgtcgagc cattggggat
     2161 ttgctccgcc actttgcggg caatcctctc tttttcttcg gtctgggtgg ctttacgatc
     2221 gtgccacggc gtgttaaccg aactggaagg cagcgccgca ccgccatggt gacgcaggat
     2281 caggttttgc tccgccagct cattgaggtc gcggcgaata gtctgcgggc tgacggagaa
     2341 atgctctacc agctcttcag tactgacata accctgctgt ttaaccagtt cgataatacc
     2401 gttgtgacgt tgtgtttgtt tcatttataa atccctggaa ttattttcgt tttcgcgcat
     2461 tgagcgaatc aacaaaagcc atcgctaaac ccacggctaa cccggcgatg tgtgctccgt
     2521 tcgccatcga catcccaaac aaatcaaacc atccggcgac aatccagatc agcgcaaaga
     2581 taattaaccc acgttgcagg taaatgccac tttgcggatc gcgttcgcca cgtagccaga
     2641 cgtagcccat cagcgcatac accacgccag aaagcccgcc aaaccacggc ccgctgaatt
     2701 tttgctgcac atagccgctt aacagggcgc taatgagagt aataacaatt agcttaccgc
     2761 taccgaggcg tttttccacc gcaccgccga gataccacca ccagagcagg ttaaagagga
     2821 tatgcatcag cgagaagtgc attaacgcgt gggtgaagta acgccagaac tcaaatttca
     2881 gtgttggatc gaatggccag gccagccata acatcacttc ctgatcgccg agaatttgca
     2941 tggcgataaa caccaccacg caggcgatca tcaccaccca ggttaccgga cctgcgcgtt
     3001 cacgcaaggc agcaaagaaa ggataacggc gataatgcag accactgccg gtatggcccg
     3061 cctgccagct ggccgccaga taacgcggat ctgccgggtt ttcgagaaaa cgcgccagct
     3121 ccgcccgcac gcgctcggcc tgggactcat ccgccagcca gacatcgctt tggttatgtt
     3181 gttgaatcgt gaggataaca ccctgcgtcg ccatataatc aacaaacgcc tgcgccacgc
     3241 gggggttagc aaaagaggta atcatcaaca tcgttgctgt cgcttattcc acacaaaagg
     3301 ggacagtata aagcgttacg cgccgtacgc cacctctgcg ggaaactgac gctgccaggc
     3361 ttcaaaaccg ccgtcaatgc tatagaccac atcgtagccc tgttgcagca gatactgcgc
     3421 cgcgcctttg ctgctattgc cgtgataaca catcaccatc accggagtgt caaagtcgtt
     3481 atcacgcata aacgcgccca gcgtgtcgtt ggttaaatgg aaagcctgca ccgcatgccc
     3541 catagcgaaa ctctgtggat cgcgaatatc gaccagcacc gcctcttttt cctgcaactt
     3601 ctggtgcgcg tcggcaacgt taatacattc gaacagatcc atgcgtctct ctttctttac
     3661 aaacaagtgg gcaaatttac cgcacagttt acgtcgaagc ggcagataaa cgccataatg
     3721 ttatacatat cactctaaaa tgttttttca atgttaccta aagcgcgatt ctttgctaat
     3781 atgttcgata acgaacattt atgagcttta acgaaagtga atgagggcag catggaaacc
     3841 aaagatctga ttgtgatagg tggcggcatc aatggtgctg gtatcgcggc agacgccgct
     3901 ggacgcggtt tatccgtgct gatgctggag gcgcaggatc tcgcttgtgc gacctcttcc
     3961 gccagttcaa aactcattca cggtggcctg cgctaccttg agcactatga attccgcctg
     4021 gtcagcgagg cgctggctga acgtgaagtg ctgctgaaaa tggctccgca tatcgccttc
     4081 ccgatgcgtt ttcgcctgcc acatcgtccg catctgcgcc cggcgtggat gattcggatt
     4141 ggtctgttta tgtacgatca tctgggtaaa cgcaccagcc tgccgggatc aaccggtttg
     4201 cgttttggcg caaactcagt gttaaaaccg gaaattaagc gcggattcga atattctgac
     4261 tgttgggtag acgacgcccg tctggtactc gccaacgccc agatggtggt gcgtaaaggc
     4321 ggcgaagtgc ttactcggac tcgcgccacc tctgctcgcc gcgaaaacgg cctgtggatt
     4381 gtggaagcgg aagatatcga taccggcaaa aaatatagct ggcaagcgcg cggcctggtt
     4441 aacgccaccg gcccgtgggt gaaacagttc ttcgacgagg ggatgcatct gccttcgcct
     4501 tatggcattc gcctgatcaa aggcagccat attgtggtgc cgcgtgtgca tacccagaag
     4561 caggcctaca ttctgcaaaa cgaagataaa cgtattgtgt tcgtgatccc gtggatggac
     4621 gaattttcca tcatcggcac caccgacgta gagtataaag gcgatccgaa agcggtgaag
     4681 attgaagaga gtgaaatcaa ctatctgctg aaagtgtata acgcgcactt taaaaaacag
     4741 cttagccgtg acgatatcgt ctggacctac tccggtgtgc gtccgctgtg tgatgatgag
     4801 tccgactcgc cgcaggctat tacccgtgat tacacccttg atatccatga tgaaaacggc
     4861 aaagcaccgc tgctgtcggt attcggcggt aagctgacca cctaccgaaa actggcagaa
     4921 catgcgctgg aaaaactgac gccgtattat cagggtattg gcccggcgtg gacgaaagag
     4981 agcgtgctgc cgggtggtgc cattgaaggc gaccgcgacg attacgcagc tcgtctgcgc
     5041 cgtcgctatc cattcctgac tgaatcgctg gcgcgtcatt acgcacgcac ttacggcagc
     5101 aacagcgagc tgctgctcgg caatgcgggg gcgataagcg acctcgggga ggatttcggt
     5161 catgagttct acgaagcgga actgaaatac ctggtggatc acgaatgggt ccgccgcacc
     5221 gacgatgccc tttggcgtcg cacaaaacaa ggcatgtggc tgaatgcgga tcaacaatct
     5281 cgtgtgagtc agtggctggt ggaatatacg cagcagaagt tgtcgctggc gtcgtaagga
     5341 tgatgcccgg ttgaagtgca accgggctat ttggattcct tagactattc attgtaaata
     5401 attgaataac ggcccctgag ctaaaatcgc caaacttaag cacgttaccc aatcgcccca
     5461 cttcttcctc tggtttctat tttttctgca ccaatggcaa taatatcccc atgcaaaacc
     5521 aagctgtgca ctaatcgggc cgacaaaatc agtccaaggc tgcggccaaa aaggggaaaa
     5581 attcagggga ggccagacaa acgtcagaaa gaaatttcgg atattttcca gcaacgctga
     5641 tttctctgga gaaatataaa gaatgaaatt cgagagaatt aaccagaaat agaccggcga
     5701 aaagaccagc cagcaatgca gtctgttttt taattcagga ggcgttctgc gggagaattg
     5761 ccaaccgatg ccagccatta cactgaaaaa aagtgcatat tcaaccatta actcaagttg
     5821 taattcctga agagaaatac gcacaaccag gagcggaaag gagaatatta tttctgccag
     5881 aaaagttaac gtgaatataa gtacggctaa gacaataacc gtcggccata ccaataatgg
     5941 cacgataaca aaaccgagaa ttacaggggg aatataaagc cggaatttaa tcatttttat
     6001 tttcctggtg attcggatga tgcgtcatac atcgcgcata aacactcatc aattatccag
     6061 tcatcttccc agacctgata ttctgtcatt ggttcgggct gttttagcgg gttctcaatc
     6121 gcttgccgcg ctctttcttt cagtaaagga tgattatttc tagcccaggc attgatttgg
     6181 gtggtaatgg cgttatcgat atcgtcctgg ttatctctga tataatcctc acaatatctg
     6241 ttcaataaac aggcaagaga aacggtcgac caaaagccgg gaactgaatt aatggcatcc
     6301 agcaatggcg caagagcgat atcggcacgg aaaaacattt ccagcatata ctgtaattcg
     6361 acagcttcat cgccggatac ccaatcagtc atatactggc aggcaaatcg ctgtaaaatt
     6421 gcccgttcat ccgcacgcca gttttctttg ttggcaaggt tgatacggcg cagagaaaaa
     6481 actacgtgaa tattgtcaaa atgcccctgt gcaacaaatt ccagtaaacg cggcagaaaa
     6541 tagcggacct catcgctatt ttgcggatca ggccctgggc tactattcca ggcattgatt
     6601 aaggaatacg ggatcgctcg taacgatgta ctccgtaatg ccttttgttc ctgctgactc
     6661 aggcaatatt cacaacaaac cacaaatttc tgcggcaccc gataacgacg aaaggtgtga
     6721 taaatctgct caattaatgc ctgcatggcg gggctgggtt gcatagttac cccctcgccc
     6781 gctgatcgcg tgtaaacggt tgatagacaa aacgagcatt cgccgcgttc aggcgaataa
     6841 gggcgctacg cgaaagggaa aagctggggc gaatgcaact attgcagtct cgacagcttt
     6901 cctctgcgca attttccact gagtaatgac gcgtgtagag tttcagttcg gcctgcttat
     6961 cgcgaaatcc gcgtaaactg ttattgttat gaaatttcca ggcatcttta aagacctgaa
     7021 agatatagac atcctcaatc caggagtcac attttttaag cgggcgcttt gctattatca
     7081 cccgctcatc cggatttagc atcgccgtaa attgtacggt gacgtcattt gtcgtgttca
     7141 ggtcacattg aacatctggt acatcactga tataggggtc aaatccaaaa tcagcaccac
     7201 aggcacaaag acggctaatg gtggcggtat cgagggaaaa tcccggccat accttgctgg
     7261 aggttaagcc aatatttaat gtgacatcat atcgaagcaa tccctcaaga ccgtcagcac
     7321 cgacgaactt ttccccttca tccagggcct ggttaataag ttgctgaaca atgccttcat
     7381 cccagtatgc cgatttgtag ttggcaatcg taatatccac gcctgtgaag ggatcagtac
     7441 cggcaaggct tgcaaataat gtattaacta attccatttt tattatccag ctttaaggta
     7501 gcgtcttata catcgggaca taacctacat cttccaccaa actctgtccc tgcggcgtta
     7561 aaaaccactc caacaatttt tgtgtctctg acgtcgtatt ttcccgcgtt accataaagg
     7621 catcgacgat atagggatat ttgccgttgc gaatgttttc ggcagtcggt gcaataccgt
     7681 taatcgctag cagttttata tttttatcgg cgttcatttg tgttgcgtaa taacggaagg
     7741 tgtaacctat tgcgttgttt gtattacggt attcggcaac gactttaatc attccctcca
     7801 tcatacttgc cacttctgtt tcctgcggcg agatcaggcg gacatttttc atgacctggg
     7861 attgcatcac cgtctggcta ccagagtctt ccgggcgttg ccaggtctgg atctcctgat
     7921 cgttaccgcc aacagtacgc cagttggtaa ttgcaccact gaagatgtca cgcacctgtt
     7981 gttcagtcag ggaattaacc ggattatccg cattgacgat gaaaacaaac gcttcacggg
     8041 caaaaggcgt gtacagcaaa gtaacgcccg attcctcagc gcgttttttc tgcccaccgg
     8101 aaggttgcgc cacaaaaata atatcagcat cacctttaac aatcctgtta tacgcatcgg
     8161 gggtacgaga gctctccaga tattccctgg tgtgaaaatc ttccggtata acgcttaaag
     8221 cataaaatgc agaagcataa attgggtacg ccgccgtagc accatcgagt cgcggccagt
     8281 tttgcgtaaa ctgaatttgc ggtttaccac gcagtggtgt cagttgatta ttgagtttat
     8341 cgggtcgcca ggcccaggta tcaatatttt cgcgcacacc gacgccggga ttgagctgtt
     8401 tatataaata accctgataa ccagcgacaa aaccaagcat gagaaggata aacaggaggg
     8461 aattacggca acgtagcccg gatgtaccgg ttaaaggatg tctggttggc cggccgtacc
     8521 ccagcgaaaa actgatatgc gcgccaacgg gtataatgac tatccaccag tactcacctc
     8581 cgactactga gactaacaaa acgcccagcc aggggagcat aagatcagcc aaccaacccg
     8641 atcccatctg gtgaaattga taatctgcca gatgcagcgc gattatccag ccaaccagga
     8701 ttaataaacc gggtactaaa aaaagacatt gtcgccagac ggctatttct cttttttctg
     8761 gtgcactatc gcctgcccat actcgccccg agaaaaatag cattaatgcg cccagcgcca
     8821 gcataaaaat gttgagcaga aggtaacttg ttggcgtgat aaggatatta caaacttcaa
     8881 taataccggc aagtccgaca cccagcatag caacaaccgc cgccaaaaat tgcgtcagta
     8941 tggggatgcc gaaaaaagtc attaccagcg aggacaaaat ccattttctg ttttgcatta
     9001 ttctttccat tctttttgaa tggtgaaatt atactccccg agtccccttg ccccttctgg
     9061 acacttttcc gaaatgatgg cggaaaaaaa cgggaccctt tggccccgtt ctatttattg
     9121 gtgaacttac aatctcaccg gatcgatatg ccagatatga tcggcgtact ctttgatagt
     9181 acggtcagaa gagaagtagc ccatattggc aatgttcagc atcgcttttg cggtccactc
     9241 ttcctgaagc tcgtagagtt cgtcgacttt atcctgacaa tcgacatagc tgcgataatc
     9301 cgccagaacc tgatagtgat cgccgaagtt gatcagcgaa tcaaccagat cgcgatagcg
     9361 acccggatct tccggactga atacaccgct gccgatttgc gtcagcacct gatgcagctc
     9421 ctcatctttc tcgtagtatt cacgcggttt gtagccctga cgacgcagtt cttccacttc
     9481 ttccgcagtg ttaccaaaga taaagatatt gtcagcaccg acatgatcca gcatctcgac
     9541 gttcgcaccg tccagcgtac cgatagtcag cgcgccgtta agcgcaaact tcatgttact
     9601 ggtgccggaa gcttccgtcc ctgccagcga aatctgttca gacagatctg ccgccggaat
     9661 gatcaactgc gccaggctaa cgctgtagtt cgggatgaac acgactttca gcttatcgcc
     9721 aatctgcgga tcgttgttga tcactttcgc tacgtcattg atcaaatgaa taatgtgctt
     9781 cgccatgtaa taggccgaag ccgccttacc gccaaaaata ttcacgcgcg gtacccactt
     9841 cgcatccggg tcggccttga tgcggttata gcgggtaatc acatgcaaca cattcatcaa
     9901 ttgacgtttg tattcgtgaa tacgtttgat ttgtacatcg aacaacgcct ttggattcac
     9961 caccacattc agctgctggg cgatatactc tgccagacgc tttttgttct ccagcttcgc
    10021 ctgatgcaca gcgtgattaa ccattgggaa atcacagtgt tgttgcagct cattaagcag
    10081 gctaaggtcg gtgcgccagt tacggcccag gtgttcgtcc agcacggctg aaagcgatgg
    10141 gttcgctacc gctagccagc gacgcggcgt cacaccgttg gtgacgttgg tgaaacgacc
    10201 cgggaagatt ttcgcaaagt cggcaaacaa cgattgcacc atcagattag agtgcagttc
    10261 cgatacaccg ttaactttgt ggctcacaac aaccgccagc caggccatac gcacacgacg
    10321 accgttggat tcatcaatga tcgacgcccg tcccagcaga tcggtatcgt tcggatactg
    10381 ttcctgcaag gttttcagga aatagtcgtt gatttcaaag atgatctgca ggtgacgcgg
    10441 cagaatttta cccagcatat caaccggcca ggtttccagc gcctcgctca tcagcgtgtg
    10501 gttagtgtag gagaagacct gacaacacac ctcaaacgcg tcgtcccagc taaattggtg
    10561 ctcatcgatc agcagacgca tcatctcagg aatcgacagt accggatggg tatcattgag
    10621 atgaatcgcg attttatccg ccaggttatc gtaggtttta tgcaactgat aatggcggct
    10681 taaaatgtcc tgaatggtcg aggaaaccag gaagtattcc tgacgcaggc gcagctcacg
    10741 cccggagtag gtggagtcat ccggatacag tacgcgagat acgttctcgg agtggttttt
    10801 atcttccact gccgcgaagt agtcaccctg gttgaattta ccgaggttaa tttcgctact
    10861 ggcttgcgca ctccacaaac gcaacgtgtt ggtcgcgtcg gtgtcgtaac cagggattat
    10921 ctgatcgtaa gcgactccca gaatctcttc ggtttcaatc cagcgcgttt ttttaccttc
    10981 ctgctgaatg cgaccaccaa aacggacttt atagcgcgtg ttgtggcgtt tgaattccca
    11041 cgggttaccg tattccagcc agtagtctgg cgactctttc tggctaccgt taacgatgtt
    11101 ctgcttgaac ataccgtagt catagcggat gccgtaaccg cgccccggca accctaacgt
    11161 cgccagagaa tcaaggaagc aagccgccag acgtcccagg ccaccgttac cgaggcctgg
    11221 gtcattttct tcatcaatca gctcttcgag atttaacccc atcgcttcca gtgcgccctg
    11281 tacatcttcg taaattccta gcgacaacat ggcgttggag agcgtacggc caatcaaaaa
    11341 ctccatcgac aggtagtaaa cctgacgagt ttcttgcgac aactgggcac ggtttgaacg
    11401 taaccagcgc tccacgagac gatcgcgcac agcaaataac gttgcgttca gccattcatg
    11461 tttattggcg acgaccgggt cctttccaat cgtaaacatc agcttgtaag cgatagagtg
    11521 cttaagagct tctacgctaa gcgtgggcga tgaatatgta aacggagcat tcatataggc
    11581 gtttcctgaa aactatttca agcgatagta aagctcacgg tacgacttcg ccgcgacctg
    11641 ccagctaaaa tccattgcca tagcctgacg ttgcacaaac cgccacagtg aaggacggga
    11701 ccacagtaca aaagcacgtc gaatagcccg taacagcgac caggcattac tatcttcaaa
    11761 gacaaaccca ctggcgacgc catctgcaag gttctcaaga gaacagtcag aaaccgtatc
    11821 agcaagccca ccggtgcgcc gcactaacgg cagtgtaccg tacttcaatc cataaagttg
    11881 cgttaagccg cacggttcaa aacggctggg caccagaatg acgtccgcgc cgcccataat
    11941 gcgatgcgaa aatgcttcgt gatagccaat ctgaacgccc acctggccgg ggtattccgc
    12001 tgccgccgca aggaaacctt cctgcagcac cggatcgccc gcgccgagta gcgccagctg
    12061 cccgccctgc tccagaaggc ccggtaaggc ttccagcacc aggtcgagac ctttctggct
    12121 ggtcagacgg ctcaccactg caaaaagcgg cactttatca tcaaccttaa gccccattgc
    12181 gatttgtaac tggcgcttat tttccgcttt atcttccaac gtatcgcggg tgtagcgcga
    12241 ggccaacagt aagtccgtct ctggactcca gattttctcg tccacgccgt tcagtacgcc
    12301 ggaaagacgc ccttcacggt gacgctgttg caacagacct tccataccgt aggcaaactg
    12361 cggttcggtg atctcgcgag cgtaggttgg actaaccgcc gtaatgtgat cggcatagta
    12421 cagaccggcc ttcaggaaag agatttgtcc gttgaattcc agcccatgaa tattaaagaa
    12481 tgaccatggc aattggatgt cattcatatg atgtgcataa aacatgcctt gataggccag
    12541 gttgtgcaca gtaaacaccg acttcgccgg acgcccgcgc gccgccagat acgcaggcgc
    12601 aaggcctgca tgccagtcgt gcgcatgcac cacatcagga cgccagaatg ggtcaagccc
    12661 gctggccatt tctgccccaa cccaccccag cagcgcaaaa cgcaatacgt tgtcggtata
    12721 ggcaaataag ttggtatcgt gatacgggct tcccggacga tcatagagat gcggcgcgtc
    12781 aatcaggtat atgccaaccc cgttgtaatg accgaacaac agcgtgatat gtccggcgaa
    12841 ggtatcacga cgggatacta cctgcgcatc ggtaacgcca cggcgaatat cgggaaatgc
    12901 aggcaacagt acgcgagcgt caacgccgtc tgcgatttgt gctgcgggta atgccccaat
    12961 aacatcagcc agaccgccgg ttttaagcag cgggaacatc tctgaacata catgtaaaac
    13021 ctgcattatc gctcctgttt atgccctaac ttccgtagca tttcgcgcgt taccagcacg
    13081 atgccttctt ctgaacgata gaaacgacgt gcatcttcct ctgcgttttc accaatcacc
    13141 atgccttccg gaataacaca agcacgatcg atgacgcagc ggcgcagacg gcacgagcga
    13201 cctacccata cttccggtaa caatacggcg gaatcaatgt tgcagaatga attcacgcga
    13261 acgcgcgaga acagaacgga ctgcaccacc accgaaccgg agatcacaca gccgccggat
    13321 accagtgagt taagggtcat cccgtggcta ccggagcgat cctgcacgaa tttcgctggc
    13381 ggtaatgatt cattgtaggt gcgaattggc caattgcgat cgtacatatc cagttccggc
    13441 accacagagg ccagatcgag gttcgctttc cagtaagctt ccagcgtacc cacatcgcgc
    13501 cagtacggct cggcatccgg gtcggattgt acgcaagaga gcgggaacgg gtgcgcatag
    13561 gccagaccgg cttcggtgat cttgggaatc aaatctttgc caaagtcgtg gctggagttc
    13621 tcatcacgat cgtcttcttc cagcagttca tacagatagt cggcgtcaaa gacgtagata
    13681 cccatactcg ccagagattt gctcggatcg ttcggcattg acggtgggtt agcaggtttt
    13741 tcaacgaatt cgataatttt atcgttctca tcaaccgcca taacgccaaa tgcggaggct
    13801 tcttcaatcg gtactggcat acaagcaacg gtgcaacgtg cgcctttttc gacgtgatcg
    13861 ataagcatac gcgagtagtc ttgcttgtag atatggtcgc ccgccaggat caccacgtat
    13921 tccgctttat aacggcggat aatgtcgagg ttttgggtga ccgcatctgc ggtgccgcga
    13981 taccagtttt cccctttcat tctctgctgt gctggcagca gatcgacaaa ctcgttcatt
    14041 tcttcattga agaatgacca gccgcgctga atgtgctgca ccagagtgtg ggactggtac
    14101 tgggtgatca cgcccatacg acggatcccg gagttgatgc agttagacag cgcaaagtcg
    14161 ataatgcgga acttaccgcc gaagtgtacg gccggttttg ctcgcttatt ggttaaatcc
    14221 ttcaggcggg taccacgtcc tcccgccagt atcagggcaa cagatttcaa tggcagctgg
    14281 cgcgccaaca ttaagtgatc gctcttctct aaactaacca tgactaactc cttttttatc
    14341 atctctggaa cacacacaat ccgtgtgcag gtccctgcca gacagccgta atcactgggt
    14401 tatcctctcc agcgaatggg ggaatggcgt gccactcccc agcaggtaaa acaatctctg
    14461 ttacctcaag cgtggcgtta attgcgatca aaaagcgatc cgagagcaga atttgcagct
    14521 gtttcggccc gttttgccac tcatccgtgc ttaaaggttg agcatatcga tttagccaac
    14581 ggacattgcc gtcgccttct tcccaccagc gattctccac caaagcggga atgcgcttgc
    14641 gcagatggat taacgcggcg gtaaatgcgg ttaaaccact gcttgcctgc gaccagtcca
    14701 accaggttaa ttggttatcc tgacagtagg cattgttatt gccatgctgg ctgtgaccat
    14761 gttcgtcacc ggccagtaac atcggcgtac cctgggagag caacaacgtt gttaacaggg
    14821 cgtgaatgct gtcgcgccgc cgttcaacca ggtcaagaga accgcctaac ccttctttac
    14881 catgattgtt actgtaattg ttgttggtcc cgtcgcgatt ttcttctccg tttgcttcat
    14941 tgtgtttatg gttgaagcaa acgcagtcgc gaagcgtaaa accgtcatgc gcggtgacga
    15001 gattaatcgc ggcactcggc agacgaccat tacgtttaaa aacatcgctg gaggcagcaa
    15061 aacgcccggc aaacgccccc agaggcaaat cataatgtag ccagaaacga cgggcagcat
    15121 cgcgaaaatg atcgttccac tcggcaaaca gcggcgggaa atttcccacc tgataaccac
    15181 caggagcgat atcccacggt tcagcaatta acttcacctg cgagagcacc gggcagttct
    15241 ggatagcggt aaacaacggc gcatcctgac ggaactctgg cgtacggccc atgactgccg
    15301 ccagatcaaa gcggaaacca tcgacgtggc aggtttctac ccaataacgc aggcaggcgc
    15361 tggcataatc caccaccgcc ggatgactca aattgagcgt gttgccgcaa ccggtccagt
    15421 tgtgataatc gccgtcttct cttatccaat aatagctacg gttatcgatc ccacgcagcg
    15481 agaataacgg gccgtcgagg tccagttccg cactatggtt gagcacgata tcaagaatga
    15541 cttcgatacc cgctttatgc agtgctttga ttgcatcgcg aaactcatcc agcgccgttt
    15601 ctggcgagca ggcatacgcc ggatgcagcg caaacatcgc caccgggttg taaccccagt
    15661 agttacttag ccccatgcgt tgcagacgtg gttcactggc aaactgcgcc actggcagca
    15721 gttccagcgc ggtaatgccc aattgtttca aatagttgat catcaccgga tgcccgaggg
    15781 ctttataagt gccacggatc tcgaccggga tctccgggtg caagtacgtt aatcctttga
    15841 catgggcttc ataaatgatg gtgctgcccc acggcgtgcg cggcggggca tcatcttccc
    15901 agacatagtg atcaaccacc actacgcatt tcggcgcaat ggcggcgttg tcgcgatagt
    15961 caggttcatt atgaccggcg tgcagcagcg ggttatcttt aaactccccg tcaatttgcc
    16021 gcgcgcaagg atcaatcaac aacttcgccg ggttaaagcg atgcccctcg gcgggttgcc
    16081 aggggccatg aacgcgataa ccataacgca aacccgggcg cgcatccggc agataaccgt
    16141 gccaaatgtc gccactgtgc cctggcaagt catagcgatg ttcctggcca ttggcgtcaa
    16201 agacacacag ttctacccgc tcggcatgag cggagaaaag tgtgaagttg acgccctgac
    16261 cgtcgtaatg cgcgccgagg ggagcgggtt tgccaatggc gagttgtgtc attctgcctc
    16321 ccgaaccagc cagatagtgg ccagcggtgg tagcgtcagg cttagtgaat gctgacgacc
    16381 gtggctggca atctcatcgc tgtgtaccgt gccgccattg cctgcattac tgccgtgata
    16441 gtgcatggaa tcggtattga ggatttcacg ccatttgccc ggctggttta tgccgaagcg
    16501 ataatcatga cgcggtaccg gcgtaaagtt actggcaacg atgatttcgt taccctcttt
    16561 atcgcgacgc acaaagatca gcaccgagcg ttctttgtca tccaccacca gccattcaaa
    16621 gccgtacggg tcaaaatcca gttcatgcat tgctttatgg tggcggtagg tgaggttcag
    16681 atcgcgcacc agacgctgga caccgtggtg ccagttatcg ccgccttcca acagatgcca
    16741 gtcgaggctg gcgtcatggt tccactcgcg gccctgggca aattcgttac ccatgaacag
    16801 tagtttcttg cccgggaatg cccacatcca gccatagtag gcgcgcaggt tcgcgaattt
    16861 ctgccatgcg tcgcccggca tgcggtcgag aatcgatttt ttaccgtgga ccacttcatc
    16921 atgcgacaac ggcaggacga agttttcagt gtagttgtag agaatcccga aggtcagttt
    16981 atcgtgatga tactgacgat aaaccgggtc gagcttcatg tagtccaggg tgtcatgcat
    17041 ccagccgagg ttccacttgt accagaagcc cagaccgccc atatcctgcg gacgagaaac
    17101 gccagggaaa tcggtagact cctcagccat tgtcaccgca ccggaaacct gctcaccaag
    17161 aatacggttg gtattacgca agaattcaat cgcttcaaga ttctcgcgcc cgccaaattc
    17221 gttcgggatc cactccccct ctttacggct gtagtcgcga taaatcattg acgccaccgc
    17281 atcgacgcgc agcgcatcaa taccaaaacg ttcaatccag taaagcgcgt taccgacgag
    17341 gaagttactg acttcacggc gaccatagtt gtagatcagc gtgttccagt cctgatgata
    17401 gccttcacgc ggatcgctgt gttcatacaa gttcgtgcca tcaaattcgg caagcgcaaa
    17461 gtcatcagtc gggaagtggc ctggcaccca gtcgagaatc acgttcagac cagctgcgtg
    17521 tgcggcatca atgaaataac ggaagtcgtc gcgagtacca aaacggcggg ttggtgcata
    17581 caggccggtt ggctgataac cccaactgcc atcgaaggga tgctcgttaa tgggcagtag
    17641 ttcgaggtgg gtaaagccca tccatttagc ataaggcacc agttgatcgg ccagctcgcg
    17701 gtagctcaac cagaaattgt tgtcggtgtg gcgacgccag gaacccaggt gaacttcata
    17761 aatagagatt ggcgcatcaa actgattcgc ttttttgcgc tcttcagtct gtacaacctt
    17821 ttccggcagc ccgcaaataa gagacgcggt ttccgggcgc atttgtgctt cgaaggcata
    17881 agggtcggac ttcagacgca agttgccatt ggcatcaatc atctcgtatt tatagagctg
    17941 accgttatgc gccccaggga taaacagttc ccagatgccg ctctctttac gcaggcgcat
    18001 cgggtgacgg cgaccgtccc agtagttgaa ttgcccaacc accgagaccc gacgggcgtt
    18061 tggagcccag acagagaaac gcgtacctgt gacgccatcc atagtatctg catgcgcgcc
    18121 taaggtttca tacgggcgca ggtgagtacc ttcagataat agccaggcat ccatttcctg
    18181 gattagcgga ccaaaacggt aaggatcatc aatcaggttt tgctgaccat gccagacaac
    18241 agccaactga tagcggaaaa aattcttacg tcgcggaatg acgccgctaa agaatccccg
    18301 tgagtcgaga cactccagtt ttgcgagttt gcgcccggtt ttcggttcaa tcacccacac
    18361 atcggtagcg tcgggtaaaa gggcacggac ttccagtccc gcggtggttt tatgcattcc
    18421 cagtacggaa aaaggatccg caaaatggcc tgcaattagc gcgttaatca cgtctctatc
    18481 gatacgatcg gacatgcttg tcttcctgtt ttattgtgtc accccatcca attggatttt
    18541 tgacttctgg ttgtgacatt tttttgacct gaacggcgca gcactctgtg catcctctct
    18601 gcgtcgtcct cacttcaggt aaggctgtga atactcatgt attcagccac ccttaaagaa
    18661 tagccaatgc tctatttaac tcccggtaaa tcatgaaaca tctgcgctta ctcctgtatt
    18721 acgcactaac aggggcggca tcgcgcccca gatttaatga ataaagatta cgccagttga
    18781 cgaagcatcc gacgcagcgg ctccgcggcc ccccacagca gctggtcgcc cacggtaaag
    18841 gctgacagga actctggtcc catattcagc ttacgcaggc ggcctaccgg cgtggtcagc
    18901 gtgccggtaa cggcagctgg ggttagctca cgcatagtga tttcccgatc gttcggaacg
    18961 actttcgccc acggattgtg cgcagccagc agttcttcca cggtcggaat agacacatct
    19021 tttttcaatt taatagtgaa tgcctggctg tggcagcgca atgccccgac acgcacacat
    19081 aaaccatcta ccggaattac ggaagatgtg ttgaggatct tgttggtttc cgcctgccct
    19141 ttccactctt cgcggctctg accgttatcg agctgtttgt cgatccacgg aatcaggcta
    19201 cccgccagcg gcacgccaaa gttatccacc ggcagctcac cgctacgggt taaggttgtg
    19261 actttgcgtt cgatatcgag aatagcagag gacggggtcg cgagttcatc tgccacatgg
    19321 ccatacagat ggcccatctg ggttaataac tcacgcatat gtcgcgcacc accgccggaa
    19381 gcggcctggt aggttgcaac ggacacccaa tcaacaagat cattggcgaa taaaccaccc
    19441 aacgacatca acatcaggct tacggtacag ttaccgccaa caaaagtcct gatgccatta
    19501 tttaatccgt cggtaatgac gtcctgattg acggggtcaa gaatgatgat ggcgtcatct
    19561 ttcatgcgca gagacgatgc tgcgtcaatc cagtaacctt gccatccgct ttcacgaagc
    19621 tttggataga tttcgttggt ataatcgccg ccctgacagg tcacaatgat atcgagggcc
    19681 tttagcgcct ccagatcaaa ggcatcctga agtgtgccag tggttccgcc aaaagacggc
    19741 gcagcctggc caagctgaga agtagaaaag aagacagggc gaatggcgtc gaagtcgcgc
    19801 tcttcaacca tgcgttgcat gagaacggag ccgaccatac cgcgccagcc gataaaacca
    19861 acatttttca taagcgtttt ttcctgcaaa gatgtgtgct gtataaatgt gccggtctcc
    19921 tcttggcaca tctttcacca tacaaaaagc agccaaagtc gcaagtgaaa ttaatcaatg
    19981 atagcgaagc catcagtaat gcgacttatc ctgctttgtt agcacgcaga aagtccgcgg
    20041 caattatcag ggaatttgag ttatgaatga aatcatttct gcagcagttt tattgatcct
    20101 gattatggat ccgctcggaa acctacctat tttcatgtcc gtactgaaac atactgaacc
    20161 gaaaagacgg cgggcaatca tggtgcgaga gttgcttatt gctctcctgg tgatgctggt
    20221 gttcctgttt gcgggtgaga aaattctggc atttctcagc ctgcgagcag aaaccgtctc
    20281 catttctggc ggcatcattc tgtttctaat cgccattaag atgattttcc ccagcgcttc
    20341 aggaaatagc agcgggcttc cggcaggtga agagccattt atcgtgccgt tggctattcc
    20401 gttagtcgcc gggccgacta ttctcgccac gctgatgttg ctgtctcacc agtacccaaa
    20461 tcagatggga catctggtga ttgctctgct gctggcctgg ggcggcacct ttgtcatcct
    20521 gctacagtct tcgctatttt tacgtctgct gggcgagaaa ggggtgaacg cacttgaacg
    20581 cctgatggga ttgattctgg tgatgatggc aacccagatg ttcctcgacg gcattcgaat
    20641 gtggatgaag gggtaacgag gagagttttt cgaaaagaac ccggtagcgt cacaactacc
    20701 gggcgaacaa acttaactca acagctggaa cgcaatcatc ccaacgatgg caccgacagt
    20761 gccgaggatg gtttccatca tggtccaggt tttcagcgtt tcggcttcgg tcgcgccggt
    20821 aaatttaccg aacaaccaga aaccggcgtc gttaacgtgg ctgacaacaa tcgaaccacc
    20881 agcgatacaa atcgacagcg ccgccatttg cgcaccggag tagttcagtt gttcaataac
    20941 cggcatcacc agtcctaccg ccgttaaaca ggctacggtg gcagaaccct gaatgatgcg
    21001 cactgcagcg gccagcacga agcaggtgat ggctatcggc aggcccatgc cagttaacgc
    21061 ttcgcccagt accggaccta cgccagagtc aaccagcacc tgtttgaaca cgccacctgc
    21121 accaatcacc agcagaatga tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac
    21181 tttgtctttt ggcatgccct gacgcattgc caggccataa atcgctacca gacaagcaac
    21241 cagaatcgcg gtaaacggat gaccaataaa ctcgaaccat tcgtaagcgg tagagccttc
    21301 tggcacaaaa cgcgcggcaa tggttttcag ccccaccagc accagcggca acaggatcag
    21361 cgacaggctg aatccgaaag atggcatttt gccttcgccg agatgcggtt cgctgatgtc
    21421 gtcaggaata tgcaactcca cgtagcggct gatgaaatta ccccacagcg gcccggcaat
    21481 aatcattccc ggaattgccg cacacaggcc aatcaggatc atccagccaa aatcggcgtt
    21541 catctgcgat gccagcagca ttggcgctgg ccctggcacc aggaacgccg ccgctgccgc
    21601 cacgcctgcg aataatggga ttaccagctt caccaggttc gtaccggtgt ggcgcgccat
    21661 tgagaaagca acgctaatca gcagaacaat cgccacttca aagaacagcg gtagcgcaca
    21721 gaccagcccc gcaaggccga tggcataatg cgcgcggctg tgaccgaagg atttaagcat
    21781 tttgacggca atctgatcga ctgcgccggt ttcatgtaag atcttgccaa acatagctcc
    21841 cagggcgaca accaccgcca ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc
    21901 tgcgatttta tcgagcggca taccggaaaa aaggccagcc cccatggaca ccaccatcaa
    21961 agccaggaaa gcgtgcatac gcgccttcat gactaaaaac agcagcagta aaacagaccc
    22021 tactgctgtt aaaacaagcg ttaatgtagt cactacttat ttgccttttt taataacctc
    22081 aatggtgctt gccacaacac cttccagcgg ttgatcgata tccaccacca gtacatcagt
    22141 ttcgtccgca cccggctcct gcagcgtttc aaactgcgtc accaacattt gggttttaaa
    22201 gaaatggcct ttgcgcgctt tcaggcggct ttcaatcacg tcaaaatcgc ctttcaggta
    22261 gatgaaagag agattcggat taccttcacg cagcaagtcg cgatagtgtt ttttcaatgc
    22321 agaacagacg atcagtgaca ctttattagt gcgctgcata gcaaacgcgg cgtcgttcag
    22381 cgcctgcaac cacggtttgc gatcgtcgtc attcagcggt tcgccagacg ccattttttc
    22441 gatattgcgc cgtggatgga ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc
    22501 cacttcactg gcgaccgcag atttgccgct gcccgatacg cccatcaaga cgtaaatgtg
    22561 gtgatcatgg ttagtcgtgc tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta
    22621 cgggtaactg ttatcggtaa cattgtccag ccggacaata tcagaagcaa tatccatccg
    22681 tgccttaagt gtataagtgt gagctacttc aaatttgtag gcttaaatag atccgcccgg
    22741 tgacaaggtg aaacctaaat ctaacatttt cggtgtcaca gattcgccac gaatacgcgc
    22801 cagcaggcgt tcagcgccaa tactgcccat ccgctcacgc ggcgtcagca cgctcgctaa
    22861 ccgcggctcc atcacctggc caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc
    22921 aggaaccttc aatcccagac gctgacattc aaacgccgcg ccgaccgcca ggtcatcgtt
    22981 ggtacagaac acgccatcca gctgcggata ttcccgccgc gcctggcgaa tcagttcaat
    23041 accggaagag taagaagaag attgctcaac catcacgcta tacggcacca gacctgcatc
    23101 cagcatcgcc tgttcgtatc ccttctgttt gatgatagta cgttcgtcga gacgcgcgcc
    23161 gagataggca atgtggcaat gcccgcgagc aataatggcg gtggtcatct ggcgtgccgc
    23221 ttcaaagtta tcaaaaccga cggcgatatc gaggcacggc gactggctgt ccatgagttc
    23281 caccaccggg atccccgcca cttcaatcat ctttaaggtg cgcggcgtgt gggtacgttc
    23341 ggtgaggatc agtccgtcga tattccatga gagcatggat tcgagacgtt cttgctccat
    23401 ttccggttta taaccgtagt gcgccagcat ggtctgataa ccgtgcgcgt cggtgacgct
    23461 ttcgattccg cgtaatactt ccgcgaaaac ctggttggtg agagaaggta acaggacgcc
    23521 aatcgcccgg ctggtggcgt tagagaggat atcgggcgca cgattgggaa tatagcccag
    23581 ttcatcaaga gcggcggcaa tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg
    23641 taaaaaacgg ctgaccgtca ttttggtcac gcctacacgg tcagccacat cctgaagtac
    23701 gggtcttttc tttttcatcg tcctgaaggt acaaaagaga tagattctct tagtttaaca
    23761 cggacgcatc agaaccttcc cggggaaaac agtgcttgtt tacaattatt agatatacac
    23821 taaatcaatt gcatcgcatt gtgctaacga cgcggcaaaa ccccggaagc gtgcatcagc
    23881 acgcgaccgg agcgagcgag gaagcctgca aaaaggcagg cttcaaaaat gccgtggagc
    23941 tttaaaccgg cggcagatcg aacagtaaca cttcgctgtc gctatccgca tggatggaga
    24001 ttgcctgctc atcccagatt gccagaccat cgctggtcga ggctttcacg ccgttaatgg
    24061 tgacattgcc tttcaccacc tggatccaga cgcggcgttc agcggcaatc tgatgcaccg
    24121 actgctcatc tttcagcaac gcccagcggt acagttccat atcctgatgc actttcaatg
    24181 aaccatctcg cgcatccggc gagagcacca gctgtttgcc ctgtacggca tcgaagcgac
    24241 gctgttcata acgcggcgta ataccgtttt cttcaggcat gatccagatc tgatacagat
    24301 gcagacgctc ggtgctgctt gggttgtact ctgagtgacg aatacccgta cccgcactca
    24361 taatctggaa ctcacccgcc ggaacctgct ctttattgcc catgctgtcc tgatgctcaa
    24421 cagtaccttc cagcacgtag gtcaaaattt ccatatcttt atgcgggtga gtgccgaagc
    24481 cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag cgcggagaag cccataaagt
    24541 tcggatcgta atagttggca aaagagaaag tatgccagga gtccagccag ccatgatttg
    24601 catgaccacg ttcatttgct ttgcgtaagt agatcatttg tattcgcccc ctgaatgatt
    24661 tcgatgggct tagtgtggac ccgatctgcc tgggatgata gagggtgaaa attgacccct
    24721 ctgttcaaaa aaattgaact attcgagggg ccatcaaact taattcgcga gagtcactgt
    24781 ggagggagag gcttgctcaa atccgcgttc aaggatttcc agattggtaa gaacttcaga
    24841 ttccttgacg taatttggcg caccgtgggt gatggtttga tacaacgcat cataaacgcg
    24901 cccgtaatcg cccatctccg gcttcatctc ttctctgacc gtcacgccct cgtcattgac
    24961 atactccagc acaccgaccg aattatccgc tgcgaatccc ggttcgcccg gcataatatt
    25021 agccttcagg ctggtttcct gctggtcgat accgtattta ataaacgaac ctttcttacc
    25081 gtgaacgata aatttcggat aatcgatttt caccagatgg ctggttttga cgatggcttt
    25141 caggtcgcca taaaacagtt gcgcttcaaa ggtgtcgtca ggattggctt tattacgcag
    25201 gctgcggatg tcataagcga cgtgatccgg gcgaccgaac agagaaataa tctggtccat
    25261 tgtatgcaca ccaaggccat aaaacgcgcc atcctgcggc agcccaggtt tggtttctgc
    25321 caccgggcgg taatagtcaa aatggctttc cacttcaaca atctctccca acttgccact
    25381 ttcaatcgct tttttcgctg tcaggaagca ggagtcaaag cgacgattct gatacggcgt
    25441 gacggtcagc cctttgcttt tcgccaacgc aaacagctct ttcgcctgcg caagtgtcgg
    25501 agtgaacggt ttttcgacca gcacattttt cccggcttcc agcgcgcgct tcgcgtactc
    25561 gaagtggctg tccgcgtggg tgcagacaac aaccagctta acatcgggat cgtttagtac
    25621 ttcgtcgaga tcgctggtga aatggatatg ggaataaatg ggagcctgtt cttccggctt
    25681 cgcatggcga cgaaaaatat gcgcgacatg ccagctatcc ttgcggttaa gtacatacgg
    25741 cagatggtaa cgggtggtgc ttttgccgaa gccaataaag gcgcagttga tgaccatgat
    25801 ttcgtccttt ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg
    25861 agtgcgcata acgaacacaa gcactcccgt ggataaattg agaacgaaag atcaaaaaaa
    25921 aagccagcac ccggctggct aagtaatact ggaagcaatg tgagcaatgt cgtgctttca
    25981 ggttctccgc gagggtcttc ctgatcgcga gacaataata atcattctca ttcgcacttg
    26041 tccaacactt tttgcaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag
    26101 ggacatttac cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac
    26161 acgggattac gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact
    26221 acaggtgcct catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat
    26281 caagcaactc gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca
    26341 acagcgccca cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa
    26401 gaaccgcggc gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt
    26461 gcgggtagat cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta
    26521 taaaaaatac ggctttgaaa ttgaagggac tggtaagaag tacgcattgc gtaatggtga
    26581 atatgtcgat gcatattata tggcgcgggt gaagtaagat agtgcccttt ttctgagatg
    26641 gaaaaagggt gtcattcaaa atcgacatac cttcctttaa ggtatttatt tgcccaatac
    26701 atatattgag atttaactca taattacttc ctaaagtgta atattttatt ttttaatata
    26761 tacgcctaca atttcctgga gtaaataaat aacaattaac aagcataata ttgccattga
    26821 taaaatagca tgccataaaa ggacttttca gggatgagta atattgttta cctgacagta
    26881 acgggagaac aacaaggaag catctccgca ggttgtggga cttctgagtc tacaggtaat
    26941 cgttggcaga gcgggcatga ggatgaaata tttacattct cactcttaaa taatattaat
    27001 aatacggggc ttggttcaca gttccatggt ataacatttt gtaaattaat tgataaaagc
    27061 actccattat ttattaattc cattaac
//
LOCUS       DAZGTR010000058        27020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGTR010000058 DAZGTR010000000
VERSION     DAZGTR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.058"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..2541)
                     /locus_tag="ACHLCY_002654"
     CDS             complement(<1..2541)
                     /locus_tag="ACHLCY_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0369886.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDNGGRSIHFEPLFPGEISYSRSESLWLARGGVAEQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLTFHRAAEGDVAGAVTGVTDGAGRCFHLVLTTQAQRAEAFRKQRATSLSSPAGPRSA
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLAAET
                     SRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGRETRYEYSAAGDLTATVSPDGKRSTI
                     AYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTRKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TSEGHRVSVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAY"
     gene            complement(2617..4758)
                     /locus_tag="ACHLCY_002655"
     CDS             complement(2617..4758)
                     /locus_tag="ACHLCY_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0369887.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGGSDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            complement(4968..5486)
                     /locus_tag="ACHLCY_002656"
     CDS             complement(4968..5486)
                     /locus_tag="ACHLCY_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0369888.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            6183..6683
                     /gene="tssB"
                     /locus_tag="ACHLCY_002657"
     CDS             6183..6683
                     /gene="tssB"
                     /locus_tag="ACHLCY_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0369889.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            6718..6942
                     /locus_tag="ACHLCY_002658"
     CDS             6718..6942
                     /locus_tag="ACHLCY_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369890.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            6993..8468
                     /gene="tssC"
                     /locus_tag="ACHLCY_002659"
     CDS             6993..8468
                     /gene="tssC"
                     /locus_tag="ACHLCY_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0369891.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            8475..8888
                     /gene="tssE"
                     /locus_tag="ACHLCY_002660"
     CDS             8475..8888
                     /gene="tssE"
                     /locus_tag="ACHLCY_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0369892.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            8892..10742
                     /gene="tssF"
                     /locus_tag="ACHLCY_002661"
     CDS             8892..10742
                     /gene="tssF"
                     /locus_tag="ACHLCY_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0369893.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVNEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            10706..11787
                     /gene="tssG"
                     /locus_tag="ACHLCY_002662"
                     /pseudo
     CDS             10706..11787
                     /gene="tssG"
                     /locus_tag="ACHLCY_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            11812..13092
                     /gene="tagH"
                     /locus_tag="ACHLCY_002663"
     CDS             11812..13092
                     /gene="tagH"
                     /locus_tag="ACHLCY_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP0369894.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAVRCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            13089..13613
                     /gene="tssJ"
                     /locus_tag="ACHLCY_002664"
     CDS             13089..13613
                     /gene="tssJ"
                     /locus_tag="ACHLCY_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0369895.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            13616..14947
                     /gene="tssK"
                     /locus_tag="ACHLCY_002665"
     CDS             13616..14947
                     /gene="tssK"
                     /locus_tag="ACHLCY_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0369896.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LTLPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            14952..15713
                     /gene="icmH"
                     /locus_tag="ACHLCY_002666"
     CDS             14952..15713
                     /gene="icmH"
                     /locus_tag="ACHLCY_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP0369897.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMTEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            15722..18517
                     /gene="tssH"
                     /locus_tag="ACHLCY_002667"
                     /gene_synonym="clpV"
     CDS             15722..18517
                     /gene="tssH"
                     /locus_tag="ACHLCY_002667"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0369898.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESESESEQDNTGAEPADEAGSEQPEETAETVSPVQ
                     RLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWL
                     GDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYG
                     GRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKA
                     HPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLY
                     PVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVT
                     DEIMSRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTAD
                     VEDAQNDESVTKDETVL"
     gene            18514..19257
                     /gene="vasI"
                     /locus_tag="ACHLCY_002668"
     CDS             18514..19257
                     /gene="vasI"
                     /locus_tag="ACHLCY_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP0369899.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            19262..20674
                     /gene="tssA"
                     /locus_tag="ACHLCY_002669"
     CDS             19262..20674
                     /gene="tssA"
                     /locus_tag="ACHLCY_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0369900.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWHQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADREPSLLEQLEQKFTAEQ"
     gene            20783..24216
                     /gene="tssM"
                     /locus_tag="ACHLCY_002670"
                     /pseudo
     CDS             20783..24216
                     /gene="tssM"
                     /locus_tag="ACHLCY_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            24227..25676
                     /locus_tag="ACHLCY_002671"
                     /pseudo
     CDS             24227..25676
                     /locus_tag="ACHLCY_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            25603..26085
                     /locus_tag="ACHLCY_002672"
     CDS             25603..26085
                     /locus_tag="ACHLCY_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0369901.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            26129..>27020
                     /locus_tag="ACHLCY_002673"
     CDS             26129..>27020
                     /locus_tag="ACHLCY_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369902.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYV"
BASE COUNT         6213 a         7542 c         7286 g         5979 t
ORIGIN      
        1 atatgcggca ttactgcctg ccctgctgcc gaagctgcgc accgtctcac ggtgcagcct
       61 gtcccgcgta tactcgacca gcggcgtccc gcccagcttc atgcccgcca ggtaaccgct
      121 gccgtaggtc agccactcca ccggcggcag gctgtccggc gtgacgcggt ttgccagccc
      181 ctgttcgttg taggcgtgtt tcgtctcatg ctgccacagc agttcccccg tctccgggtt
      241 ctccaccgtc tgccgttcgc ccgtcaggcg gcccttatcg tcgtacccgt agtggaccga
      301 cacgcggtgg ccttcactgg tatggctgag ggtggttaac cacccgtgct catcatactg
      361 ccactgctct gccgggtcgc cgttcaccgt ccggtgcgtg atgcggtccg acgcatcgta
      421 gtgccagaga gtgataagcc cctcgtcttc actctgtgtg agttttctgg tcaggtcata
      481 gtggtaacgt tgcgtccggc cgtcaaaacc gcgctgttca gtcagcctgt caaccgggtc
      541 ataccggaac gtggactggc tgccgttctc gttggtcagc acggtgatgc gcccggcagc
      601 gtcatacccc atgctgcgcg tcagaccgcc ttccgtcaca ctcaccggac gcccgcgttt
      661 atcatatgcg atggtgctgc gtttaccgtc cggtgatacc gttgctgtca ggtctcctgc
      721 ggcgctgtac tcataacggg tttcacgccc ctgcgcatcc ttctgactga ccagttgccc
      781 acgcgggtta taactgctgt aagtgctgat gccttcctcc cggtgaacgg cgatttgttg
      841 accgtaccgg tcgtactcat accgcgtcgt gtaccccgag cagtccgtaa aggccagcag
      901 ttgaccgtaa cggctccatg ccatctgttt tgtactgccc gtcgcgtcct gtatccctgt
      961 cggcagctca ctcgccgggt catcatagct gtaactcgtc gtctcaccgc tgcgtgaggt
     1021 ctccgccgcc agccttccct tctcatcata ctcccggctg ctgcgaagcc cgtccgggta
     1081 cgtcaccgac gtcacctgcc gctggctgtt atagccatac cgtaccgtcc tgccgtccgg
     1141 ccccgtcacc gccgtcaccg cacccgacgc catatgcagg ctgtactccg tccgccgccc
     1201 cgccgcatcc gtctgtgcct tcagcctccc cgcctcatca tactcgctgc gggtaatgct
     1261 cccgtccgca tgttccttct tcaccacccg cttcaggccg ccctcgccct ccgtgtacag
     1321 cacctcccgc cggttcaggc tgtccgtgat ggtcacacgg tcctgcccgt actcaaagcg
     1381 gtagtccagc ccctccgggt tgacctgctc cgtcacccga ccggtatcat cataccggta
     1441 gcggctctcc ggcctccccg cataatggtg tgccaccatc cgcccggcgt gctccgcatc
     1501 ataagtaaac ccgcgaacct gcgtcccgct gcggtcatac accgcccgca gttctccgct
     1561 ggccgtgtac gtgtagcgcg ccagcggcgc agtcggctgt tcatccgggt atgccgggtc
     1621 gtgtgtcagc cacaccgcct ccagccggat accgttatcg gcaccgtatt ctgtaccggc
     1681 gggcagcgtg tcggggaaag cagaagaaga ggaagcagaa cggggaccgg caggggaaga
     1741 taaagacgtg gcgcgctgtt tacggaaggc ttccgcccgc tgcgcctgtg tggtcagcac
     1801 caggtgaaaa cagcggcccg cgccgtccgt cacccccgtc accgcgcccg ccacgtcacc
     1861 ctccgccgcc cggtggaaag tcagcgtccg cccgaagccg tccaccacgc ccgtcagcac
     1921 ccggtatgcg ggcggctccg gcggcagcac ctcgtccgcc cccggcaccc gctccggcca
     1981 gctgagtatc caccacggcc cctgaaggct gtttgtcgcc aggtacacat gcggactcag
     2041 gcgaacatct tccggcagca cctgccagag tgcgcttagc ggctgcgaac tgtgctgctc
     2101 cgccaccccg ccccgcgcca gccagagtga ctcgctgcgg ctgtagctta tctcgccggg
     2161 aaacagcggc tcaaagtgaa tgctcctgcc gccgttgtcg ttgagtatca ggccttcatc
     2221 gcgtatctgt aagcggatat cgaagggcgc tttccagccg ggaccaaaca cccccaccgg
     2281 cgcgggtgtt ctggtccggt agctgctgta ggcgcgggaa agaataaacg gcagcgggcc
     2341 gggcagcgca aggtccgtct cgcccggcag caccttcgca cccagcagcg ggttcaccgg
     2401 gttagcatag gtaatccctc ccggacatac cgaacaggcc actcccgtcg gcgcacctat
     2461 cagcaccccc gctgaaccct gcacaatatc cagtcccttg cgggtcatat caccctgccg
     2521 cgccgccggt tttccgccca tgaatcaatt ccttctgctt ttaatatggt ttatctggtc
     2581 actgcaccag ttccgtttta attttttgca cctgactcaa aattgccgca tatccggcat
     2641 tccggggggt aaatctttcg gcacaggcgc atccggtaca ggttgtgtgg caccacagtt
     2701 ccagttaatc actggcgcat caccgttcac atccgacccc ccttccagat gaatgtgcgt
     2761 accattgaga aatatgcttc cgtccttcgt cagcaccagc cttgccttac cacagcagag
     2821 ttcaagatgc tcaccggcag aataaactgc tgtttgtccg gtgttttcct tcattgtctt
     2881 gcccaccgag aaggtgacgt tattccccac attgacgctg tagcccattc ccaccatcag
     2941 tgatttatgc agccctacct gtgaggactg caacaacgcc accgaggtat tcataatgcc
     3001 gcctaccgtc gtctggtaag ccacacccac cgtcagcgca cgaatgatcc ccaccttttc
     3061 aacctggttt gatcccactt tgataatctg gttactgccc accgtttcca cctggttgac
     3121 accaaccgtc tgtatctgat taacgccaat gttctggatc tggttattgg taacggtgat
     3181 cgcctggttg ttacctattt tttcggcatg gttgtttatc acatcagtgg tgcggttatt
     3241 cagcacctcg gtgttcatgt tcttctgcgc gtggatgtag acctgttctt tccctgtcgc
     3301 atcgtcaaac ttcagttcat taaacccgct gcccttatag gttttcgagc ggatcgtcat
     3361 ctgcgtcttc gtccccggca ggctgccggg ggtgcggttt tcctggtggt aggtacgccc
     3421 cataatgatc ggctggtccg gatcgccgtt gaggaagtcc acaatgacct cctgacccac
     3481 acgcggtatc gccaggttac caaagccggt gcctgcccac gcctgtgcca cacggatcca
     3541 gcatgaactt tcctggtttg acgggttata gcggtcccag ttaaatttca cccgcacgcg
     3601 gccatgttca tcacagaaga tttcctcgcc tgccggtccc gtcacgacgg cgctctgcgg
     3661 gccgtccacc agcggtttca gcaacggctg tggccgccat gtccggtctg ccgggattac
     3721 cgcaaagtgg ttatccagcg tggtgcctga acccctgcgt cccggtaccg cctgcggctg
     3781 ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg cctgcggatg
     3841 ccccgtcagc acaattcgcc gtcccggcca tatctccggc gaacggcttg ttccgcgcgc
     3901 cacttctgcg ttgttgcgcc agccatccat ctgccagcgg gcaaagttct gcccgtgggc
     3961 acccttgaaa cgtccggggt agtcatacac ttcatactgt gtgcgctggt aatcctggta
     4021 ctggccttcc tgatcaaaac gccctgccca gccgggtcgt ttaaaggtgt agtctttggt
     4081 caccacggaa gaagggcgga tttgtgcgct gtagcgaaac tggctgatgc agagggtgct
     4141 cacctcggta cgggtgttcg gattccaggg gatctcaaag gactccggca gataacgcac
     4201 ggtatcgcac aggaccaggc tctggtcggt acttttttgc gcgtgctcct cataaaagaa
     4261 gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtctcac cgtactggac
     4321 acaaaactca cgggaaggat gtggctcgct gaacagcggg ctccactcgg tcaccccgtt
     4381 ttcctgcaat atcgtgccga ggatgctttc gatgtcctca ttctggaaga tacggaagtt
     4441 ctggcgcagc cctgtgcgcc acagcggtgg gcacaccttc atgctgtaca gcatctggtt
     4501 tttgtcgttc tcccccagtt caaaccaggt caccatacct ttcacccggc gctgtacgtc
     4561 atcgccctgc catatcgtca ggtaggccat tttgtcgagg atctgctgga attcaaggga
     4621 gagaaactgc tggctgacca gagagagatc gagggaaaaa agcgaagaga gtgactggtt
     4681 cagatgaaag gagaccaccg caaaagcatc cggtggcagg ccgtccactt ccagcgtgaa
     4741 acgtaatccg gttgacatag caacctccgg ggttaaggaa aaacagacag ttcgctgtta
     4801 aagcggctgt gacagtacag tgttacgctt gcgttcaggc acgctaccgc cgaagtttcc
     4861 ggcagccgcc atctcccgtg aacgtaaccc cttactgaca acctttcttt ctacggcgta
     4921 atacaaaaac agcatccggc ctgatagccg gatgctgttg gcttaactta tgcttccagc
     4981 ggtgcgcgcc agtcgtcggc accggaggtg ccagatttga cgtgttccca ttcaattttg
     5041 cggtaggcca gtgaaacttc gagcaactgg gtgaactcgc gtttcgccgg gtcctggcaa
     5101 tgcggcatat gcagtttcat atcgacaatc gtcgaatcag tcaggcgggt agtgaaataa
     5161 tgctcctgtt tgccttccac agaggtacgc caccagtgaa gttcagtggt tggcagcatt
     5221 tcgccggagg ccagtgcgtt gtacatcagc ggaaccgctt tgttcagcgc cacggtaaaa
     5281 atgaaaggct tgtgagcacg ctgacccgca ggctgaccgg actgaggatc ggtcgggaca
     5341 gtaacgttat gcaggaattc ctgcaccagc atctcgtctt cgtgcccctg cacatagatg
     5401 ttgccgacag aatcagcggt aaatgcacct gcagtaatat ttccctgggt ctgaccagtg
     5461 atggagatat aacacggtgt tggcatttgt aaactccttg ttaacgtggt taattactac
     5521 ttacaatgac ctgccgttaa tcccagacag gcagatgcag ctcacttcac ggaaccgcat
     5581 catttttcag tattttttac atacaaaccg agctagaatt tacggttgtc gtaaaaacta
     5641 tttttaaaat cattaaatct cttattcagc aataacttaa taatatatat ctggataacc
     5701 atacccaact gttcaaagtc cgtattgcct ccataaaatg gccaaagctg aatcatgaat
     5761 aaatattgta tttatccttt ctgaattatc attttgataa tttttgagat attccttaaa
     5821 aatacccact taatcaatca caaaacaaat atataaataa gcaatcatta gattagctta
     5881 tgtttaatga aattattata tacttcaata agttacataa aaaatctata tttaaattat
     5941 tgccataata cacctgacgc gaattgcaca ggttcacaca atctatttaa cgcttgcaga
     6001 atacccctct ttacgatcga taaaaagtcc ttttcttatt tattttgaag cgtaaaatgc
     6061 ggggtgatga aggagtggtg attttatttt caccgccatt agggcggcat tgccgatatt
     6121 tttttgcggt acaaatatgc cgtgttcatg gcctgtacga gataacatga cggatcactt
     6181 ttatgagcaa aaaatttgaa ggcagcgtcg cgcctcgcga gcgtattaat atatcgtatg
     6241 tcccaaaaac agatggccag acggctgaag tggaacttcc gcttaacatg ctcgttgtcg
     6301 gcgataccgg aaatacgcag gaaacatccc cactggatga acgtcaggca gtttccgtta
     6361 acaagcacaa tttcggtgca gtgatggctg aagcagccat tggcctcaat tttactgtcc
     6421 ccgccacgct gaagggaagc acaacagacg atgaactgaa tgtggcgctg aatatcaaat
     6481 cactggatga tttttcacca gatagcgtgg cccggcaggt tccggaagtg aataaattgc
     6541 tggaactgcg tgaagcactg actgcactga aggggccgat gggtaacctt ccggcattcc
     6601 gtacccagct ccaggcattg ctggaaaatg aagagtcccg cgagcagttg ctcaaagaaa
     6661 tcggtctggt gagcaataaa taaccaccac tatttcatta aacagggtta tccaggcatg
     6721 acggcacaca aaaatatctc cgggacatac cacctctctg ttgcagacat tctgcaggtg
     6781 gtttaccagg tatgtttcag cccgtccgtt gaaatcaacc aggatggtgt ggctgcgctg
     6841 attacaacac tggatcgccg tatcagtgat ctattggatg agattatcca tttttgtgag
     6901 tttcagcaga gcgcgtcgca ctggcagcgc gtacttcact gatggcgaaa agccggtttc
     6961 agaaacacag ctaaaacaga aggattttca gtatgtcttt acaggaagag gaactcgttt
     7021 ccagtcatgc cgggcagcct gagcaggcat cgtcgctgct tgatcaaatc atggcacaga
     7081 cccgtattca gcccggttcg gaaggttatg acgttgcccg ccagggtgtg acagccttta
     7141 ttgccagcat cctgcaatcc actgcatctg ccgaaccggt caataaactg gccgtggaca
     7201 gcatgattgc agatattgat gaacgcatca gccgccagat ggatgtcatc atccatgcgc
     7261 ctgcctttca gcaggtcgaa tcgttctggc gctcactgaa aaccatggtg gatcgcgttg
     7321 atttccgtga aaacatcaag gtcaacgtgc ttcatgtcac caagcaggaa cttctggaag
     7381 actttgagtt tgcgccagaa attattcagt ccggatttta taagcacgtt tattcgtccg
     7441 gtttcggaca gtttggcggt gaacccatcg ctgccgtgct gggcgcttat gagttcaaaa
     7501 ataccgcgcc tgacatgaaa ctgctgcaat acgtcagtgc cgtgggcgca atggcgcacg
     7561 caccgttcct gtcctccgtt tccccggagt ttatggggct gaactcgtgg accgaactgc
     7621 ctaatatcaa agatctttat gccatctttg aaggtccggc ctacaccaaa tggcgcgccc
     7681 tgcgtgactc ggaagattcg cgctatttag ggcttacggc accccgcttc ctgcttcgcc
     7741 agccctattc accaacagat aaccctgtta agaactttaa ctattacgaa gatgtcagcc
     7801 agaaccacga agattatctg tggggtaata cggcctggat gctggcatgc aatatcgcag
     7861 acagttttgc caaataccgc tggtgtccaa acattatcgg tccgcaaagc ggcggcgcag
     7921 tgaaagatct gccggtgcat ctgttcgaaa cgatggggca gattcaggcc aaaatcccaa
     7981 ccgaggtgct ggtcaccgat cgccgcgaat ttgaactggc tgaagagggt tttatcaccc
     8041 ttaccatgcg taaagactct gataatgcag cctttttctc tgcaaactcg gtacaaaaac
     8101 cgaagcactt tccgggaaaa gatgcagaaa ccaactataa gctgggtacg cagcttcctt
     8161 atctcttcat catcaatcgg ttagcgcact acatcaaggt gttgcagcgt gaacaactgg
     8221 ggtcatggaa agaaaggagc gacttagaaa gagagcttaa tacctggatc cgtcagtatg
     8281 ttgccgacca ggaaaacccg cctgcagacg tgcgcagccg caaacctctg cgcgctgcca
     8341 aagttgaggt tatggatgta gaaggcgaac ccggctggta ccaggttgcg ctaagcgtga
     8401 ggcctcattt taaatttatg ggggcaaatt ttgagctttc cctggttggc cggttagaca
     8461 gggagtaaca caccatgata ccggatcggg aaagaaccac aggttctctt tttgagcgta
     8521 tggaagcctc ttcggccaga aaccggcagg gaggcagtat tcattcgctc aggcaatcca
     8581 ttcgccagaa cctacgaaac attcttaata cccggtccgg tagttgccgg ggtgcgcctg
     8641 agctgggcat tgatgaaccg gaaggggctg aaaacttccg cgaatccatg agtcgggcca
     8701 tcgagcaatg tatagaacgc tatgaacccc ggatctcaca cgctgaagtg caggcagtgg
     8761 tttcatccgc ctccagcccg ctggatatga cgtttcatat tacagcctgg gtgacattta
     8821 acgaaacgca tgaggtgctt gaatttgata tggcaccgaa tggcagccag cattaccggg
     8881 tggactgaca aatggaattt gaagaacgct atttccggga agaactcgat tacctgcgcc
     8941 agcttagcaa gctgctggca acggaaaaac cccatctggc ccgcttcctg gccgaaaaag
     9001 atgcggatcc ggatattgaa cgcctgctgg aaggggtggc ttttcttacc ggcaatctcc
     9061 gccagaaaat tgaggatgaa ttcccagaac tgacgcacgg gcttattaag atgctatggc
     9121 ctaattacct gcgtccggtt ccggcaatga cccttattga atatacgccg gatatggata
     9181 agtcttctgt accggtgtta atcccccgta atgagcagtt tacaaccaac gccggggaaa
     9241 tcagagttaa tgaagtgctg ccctctgatg ctaaaaagga ggagccgcct ccctgtacct
     9301 tcactctctg ccgggatatc tggctgctgc ccgttcgcct ggagcagatt gaaaaccgca
     9361 gtacgacccg taatggtgtt atcaacatca ccttttcggt cgcaccggga acggacttcc
     9421 gcacgctgga tctgaacaaa cttcgcttct ggctcggcaa tgacgacaac tatacccgtg
     9481 accagcttta tttatggttc tgcgaatact tgcagggtgc cgacctgact gtgggtgaac
     9541 agcatattcg cctgcctgag tttatgctaa aagctgtcgg ttttgagccg caggatgcca
     9601 tgctgccctg gccgaaaaac gtccacagcg gctaccggat ccttcaggag tatttctgtt
     9661 accccgatgc gtttctcttt tttgatcttt gtggttgtcc ggctttgcct gacggattgc
     9721 aggcggaatt ctttaccctg caactgcgtt tttcgcgccc tttgcccgtg gacatccggc
     9781 tgcgccgcga ttccctgcgc ctgtattgcg cacctgccat taatttattt atccaccatg
     9841 cagaagccat cacgctggac aaccggcggg cagactatcc gctggttccc agccgccatt
     9901 acccacaaca ttacgatgta ttttccgtta acagtgtggt gagccaggtc caggatatgt
     9961 tcaggaaaaa agatctgggg cgtcctgttt cgacgcaggc cgcgcgccag tggccagcct
    10021 ttgaaagttt cagccatcag atggaataca gccggaagcg ggaagtggtg tactggcatc
    10081 accggaccaa aacatccctg ttccatcgcg gctttgatca tacccttgcc tttatacatg
    10141 ctgatggcag ttatccgtca gacgaatctc tgctcagtaa tgaagtggtt tcggtatcgc
    10201 tgacctgtac caaccgtgag cttccgtcac aaattcgttc cggcgatatc accggcacaa
    10261 ccggtaaaaa tgcggctgtt gcttcatttc gcaacattac ccgcccgacg caaccactct
    10321 ggccggtcat tgatggcagc ctgcactggt ccctactctc cgccatgaac ctgaattatc
    10381 tgtcattact ggatacggac gcgctgaagc aggtcatcgc caactttgat cgccacgcaa
    10441 tccatcatcc gcagacggca cggctgtcac aacaaaagct ggatgccatt gagcgtctgg
    10501 agacccgccc cgttgatcgc ctgtttacgg gtattcccgt ccggggactg gcctccacgc
    10561 tgtatctgca cccggagccg tttgtctgtg aaggggaaat gtatctgctc ggtacggtgc
    10621 tttcgcattt tctgtcgctg tacgccagcg ttaactcatt ccacatgctg accgttgtga
    10681 acacagaaag ccaggagaca tggaaatgga cggaaagaat cgggcagcat cctcttatct
    10741 gagtccggga aatcccccgg cagataaaga gcagaatgat ccgctggcac aggttttcca
    10801 taatgcctgc tcttacaatt tttttgcgat ggcggagctg ttgcaccgcc tggcaaaggg
    10861 tgaaaagggg acgccagaat tatccctgcg tgacgatccg gcacaggaaa ccctgcgttt
    10921 cagcgccgat gccagccttg ccttcccgtg cagtgatatc agtgcactga aacgggatac
    10981 gtcaggggca ttcaggatga ccaccacctt tatggggtta caggggagcc agtcgcctct
    11041 ccccggttat tacctcgatc acctggcctg gaaagcggta catgaacaaa gtccggtagg
    11101 tgattttctg gatatgttca gccaccgcct gacccagttt gtctggcata tctggcgtaa
    11161 gtaccgctat cacatcagtt ttcgtaacgg cggtgtggat gcgttctcgc agcgcatgta
    11221 ctcccttgtc gggctgggcc accgccagct tcgcgataag ctggcgatta atcacagcaa
    11281 aatgctggcg tattccggga tcctggcaaa tccgggacga tccccggaaa taatctgtgg
    11341 cctggtgtcc cactgctttg atttgtcaga agtgacgctg caaaactggc aacgtcgcaa
    11401 ggtggatatt gaactgatca gcaaaatagt ctggggagct attctcttaa aaatggcgaa
    11461 aaactggcgg gaagatcggt actgggcaac tttgttctgg gtacacgagt gcccgatctc
    11521 agcggaaaat tccagctcag tattaccagc ctgacccgaa aacagttcct ctcttttctg
    11581 ccgtccggcg aaaacttcct gccactgacg atgttcgtgt ccttcatcct gcgcgatcag
    11641 cttgcctggg acttacatct ggggctggcg ccggagcagg tgggcgcaat gcgcctgggc
    11701 gataacaaaa gtgcgctgct gggctggacc agcttcctcg gcacaccgga agaacgaccc
    11761 tcagtcacca tcagggtacg gtcgtaaatt atgataatca cggagcagaa tatgccggaa
    11821 gagaaactac agacgctttc attgcaggtc attaacggca gtgagctgga aagcggacgg
    11881 gcggtgcgct gtctgttcac acagcaggga aatgtcggcc atggccccga atgccactgg
    11941 tcggtacagg atcgtcagca gagcattccg gcccaggctt ttaccgttat cctgcacgat
    12001 ggcacatttt gtctgcgccc gcagacggca caactgtggc tgaatcaggc aaaagtcaca
    12061 gcaacatcag acctaataca gttgcgccag ggcgatgaga tccagatcgg acggctgatg
    12121 gtgagggttc atctgaaccg gggagatatt ccccattacg atgaggaaat ggccactccc
    12181 gaaaccatcg ttaccaatcg cgatatgctc acggataccc tgctatcaac ggagggtacg
    12241 ccacactatc cgggaatgac tcaccggcac caacttgcag acaccgtggt aaacggtttt
    12301 tctgccgatc cactccaggc acttcagtcc gaaagcctga ttaccacggg cgatccgctt
    12361 tcaggcattg cggctgtccg gccatcggca ccgctgtccg atccggcaag taatgggggg
    12421 atcaatactc cgtttatgga tctgccgccc atttatgcca gccccggcga tcgtaatgac
    12481 gacgtctctg cggcagaaat ggcgcaacgc caccttgcgg tcaccccctt actgcgcggt
    12541 cttggcggct cgcttaccat gagcaattcc gacgatgcgg atgattttct ggaggaggcc
    12601 ggacgaacgt tacaggccgc aataaaaggt ctgctcgatt tgcagcagca gcgtaacagc
    12661 ctctcagaca aacatttgcg cccgctggaa gataacccgc tgcgcctgaa catggattac
    12721 gccaccgcgc tcgacgtaat gtttgccgaa ggtaaaagcc cggtacatct ggcggctccc
    12781 gccgccgtca gtgaaagcct gcgcaatgtc cgccaccacg aagaagctaa ccgggcagcg
    12841 attgtggagt cgcttcgtgt cctgctggat gctttctcac cacaaaatct gctgcgccgc
    12901 tttgtgcagt accgccgcag ccatgaactg cgccagccgc tggatgatgc cggagcatgg
    12961 caaatgtaca gccattatta cgaagaactg gcctccgatc gccagcaggg gtttgagatg
    13021 ctgtttaacg aggtctacgc ccaagtctat gaccgggtgc ttcgtgaaaa acagcgggag
    13081 ccggaagcat gaacaggaag gcgtttctgg cctgcgtgct gatgtgcgtc atattaaccg
    13141 gctgcgaaac ggcgaaaaaa atcagtgagg tgatcaaaaa cccggatatc caggtcggga
    13201 gcctgaagga gcaaccttcg gaaataaccg tcaccctgct gaccgaaccg gacaccaata
    13261 ccaatgcgga aggtgaatca gccgccgtgg atgtgcaact ggtgtacctg actgacgact
    13321 caaaattgct ggcagccgac tatgaccaga tagccagtac gccgctaccg gatgtgctgg
    13381 gtaaaaacta tatcgaccac caggatttca acctgttgcc ggacaccatc aaaaccctgc
    13441 cgccggtaaa gctggatgag aaaacgcagt ttattggtgt cgtggcctat ttctccgatg
    13501 accaagccac tgaatggaaa caaatcgaga cggtggaagg aaccggccac cattaccgcc
    13561 tgctggtgca tgtacgtcaa agcagcatcg aaatgaaaaa agaggatgaa taattatggc
    13621 aaccacccgc aacaaggtga tgtggcagga agggatgctg atgcgtccac accatttcca
    13681 gcagcagcag cgttacaacg actacctgga taaccagcgt ttccgggcca tgaatgattt
    13741 atcctgggga tttaccgaac tcactctcaa caatgaactg ctggcacagg gtaagatcat
    13801 gattgacagc gcgtcaggca cactgcccga cggcaccgtc ttttctatcc ccgaccagga
    13861 cgcactgccc gatccgctgc acccgcaaca ttttccggac gagagaagcc gcaatatcta
    13921 cctcactctg ccggtcgcca gtgatgtgag aaatgaaatc agcgacgggc ggcgaatcgg
    13981 gcgttaccgg ctgaattatg ccgatgtccg ggatttgcat tcagaagaag gtgacacgcg
    14041 aacactgacg ctgggacaac tgacgccgcg cattatgagc ggtgcagaag atatgagcgc
    14101 ctatattacg ctcccgcttt gccgtatcag cgatcgccat gccgatggct ccctgacgtt
    14161 ggatgacgat tttatcccct cctgccagaa tattcaggtc agtaagaaac tgcgtgttta
    14221 tctcaaagag gtacaggggg ccattggcgg acgggcaagc gatctggcaa accgtattgg
    14281 ctctccggcg cagagcggca tcgcggatgt ggcggaattt atgatgttgc agttacttaa
    14341 ccgtaaccag acccggttta cccatcgcgc ccgccgatcc cagctccacc cggaagattt
    14401 ctaccttgat cttgctgggt tgctgggtga actgatgacc tttaccgagc cgtcgcgcct
    14461 gccctgcccg cttgatgtgt atgatcatca tgatctgacc aaaacattta aaacactgtt
    14521 accggaagtc aaacgggcgc tgcataccgt actgtcgcca agagcggtca atctgccgct
    14581 gcatctgcgt gacggtatct ggcaggccga tatccatgac acagaactgc tgcaatctgc
    14641 cacctttgtg ctggccgtgg cggcaaacgt gccggtcgat cagatccagc gtcagtttat
    14701 ccagcagtcg aaaatttcct cgccggaaaa aatccgcaat atggtcagtg tgcagatccc
    14761 cggcattcca ttgcgcgcac tgatggtggc tccccgccag cttccttacc attccgggtt
    14821 cagctatttc gaactcgaca agagcggaca ggcctggaca gaaatggctg ccgccggggc
    14881 cgttgcactg catgtatccg gcagtttccc ggatctgaac atgcaactgt gggcgataag
    14941 agggtaagac gatggatgag gggtcactgt ctctgccacc atttaccgga tacgacgaaa
    15001 aaagccagcg cgactatcat ctcgcgcttc gtggcaacag cctgaatccg atgatcgacg
    15061 cagccacgcc gctcctcggt atggtgatgc gcctttcaac catgaacagc cagacgatga
    15121 cggagcatct ttttgctcag gtggtgacgg atgttcaggc cgtggagcaa ctgcttcagg
    15181 agcagggcta cgaaccgggc gtgattatct cgtttcgcta catcctctgc acgtttattg
    15241 atgaagcggc gctcggtaac ggctggtcga acaagaacga gtggatcaag cagtcattgc
    15301 tggtccattt ccacaacgaa gcctggggtg gagagaaagt tttcatcctg ctggagcgcc
    15361 tgatccgtga acccaaacgt taccaggatc tgctggaatt tctctggctc tgtttttctc
    15421 tgggatttcg cgggcgctac aaagtggcag cacaggatca gggggaattt gagcagatct
    15481 accgccgcct gtaccacgtc ctgcataaac tacgcggcga tgcccctttc ccgctgttac
    15541 accaggacaa aaaaacccag ggcgggcgtt atcagctcat cagtcgcctg accattaaac
    15601 atattttttg tggtggcgtt gtcgtgctgg cgttgtttta cctgttctat ctgttgcggc
    15661 tggacagcca gacgcaggac atcctgcacc agctaaacaa attacttcga taaggacgtt
    15721 tatgatccag attgatcttc ccacgctggt aaaacggctt aacctgttct cccgccaggc
    15781 gctggagatg gccgcctctg aatgtatgag ccagcaggca gcggaaatta ccgtcagcca
    15841 tgtgcttatt cagatgctcg ccatgccacg cagtgacctg cgggttatta cccgccaggg
    15901 cgatattggc atggaagagt tgcgccaggc gctgacggtg gagaactaca caactgcccg
    15961 ttctgcggac agctacccgg cattttcccc gatgctggtt gagtggctga aagagggctg
    16021 gctgctggcg tcggctgaga tgcagcacag cgaattgcgt ggcggcgtgt tgctgctggc
    16081 cctgctgcat tcgccgctgc gttatatacc gcctgctgcc gcccggctgt tgaccggcat
    16141 taaccgtgac cgtctgcaac aggactttgt gcaatggaca caggagtcgg cggaatcagt
    16201 cgtgccggat gcagacggta aaggcgcagg cacactgacg gacgctgctg acaccctgct
    16261 tgcccgctac gccaaaaaca tgaccgcaga cgcccgtaac ggcaggcttg acccggtact
    16321 gtgccgcgac cacgaaatcg acctgatgat cgacattctc tgccgccgcc gcaagaacaa
    16381 cccggtggtg gtgggcgaag caggcgtggg taaaagcgcc ttaatcgaag ggctggcgct
    16441 gcgcatcgtg gcaggccatg tgccggacaa gctgaaaaac accgatatca tgacccttga
    16501 cttgggcgca ttgcaggccg gagcgtcggt gaagggtgaa ttcgaaaaac gtttcaaagg
    16561 gctgatggca gaggtcattt cctccccggt gccggtcatt ctgtttatcg acgaagcaca
    16621 taccctgatt ggcgcgggca accagcaggg cgggctggat atctccaacc tgctcaaacc
    16681 ggcgctggcg cgcggcgagc tgaaaaccat cgccgccacc acctggagcg agtacaaaaa
    16741 atacttcgaa aaagatgccg ccctgtcgcg ccgcttccag ttggtgaagg tcagcgaacc
    16801 caacgctgcc gaagccacca ttattctgcg cggtctgtcg gcggtctatg aacagtctca
    16861 cggcgtgctg attgatgatg acgccttgca ggccgctgcg acattaagcg agcgttatct
    16921 ctccgggcgt cagttaccgg acaaagcgat tgatgtgctg gataccgcct gcgcccgtgt
    16981 ggccatcaac ctgtcgtcgc cgccgaagca aatctcggcg ctgaccactc tgagccacca
    17041 gcaggaggcg gaaattcgcc agcttgagcg cgagcttcgc atcggactgc gtaccgacac
    17101 atcacggatg accgaggtgc tggtgcagta tgatgaaacg ctgacggcgc tggatgaact
    17161 ggaagcggcc tggcaccagc agcagacgct ggtccgggag attattgcgc tgcgccagca
    17221 gttactgggc gtggcagagg acgatgcggc gccgttgccg gacgcagata ccgtggagga
    17281 tacgcagcca gagtcagagt cagagtcaga gtcagagtca gagtcagagt cagagcagga
    17341 taataccggt gccgaaccgg ctgatgaagc tggcagcgaa cagccggaag agaccgctga
    17401 aacagtttcc ccggtacagc ggctggccca gctcactgcc gaactggacg ccctgcataa
    17461 cgaccggttg ctggtctccc cgcacgtcga taaaaaacag attgcggcgg tgattgccga
    17521 atggaccggc gtgccgctca accgcctgtc gcagaatgaa atgtcggtca tcaccgacct
    17581 gacgaaatgg ctgggtgaca ccatcaaagg ccaggacctg gcgattgcca gcctgcataa
    17641 gcatctactg accgcacgcg ccgacctgcg tcgtccggga cgcccgctcg gtgcgttcct
    17701 gctggctggc cccagcggtg tgggtaaaac cgaaaccgtc ctgcaactgg cagagctgct
    17761 ctacggcggt cgccagtacc tgaccaccat caatatgtcc gaattccagg agaaacacac
    17821 cgtctcgcgg ctgattggtt cgcctccggg ctacgttggc tacggtgaag gcggcgtact
    17881 gaccgaagcg attcgccaga aaccctactc ggtagtactg ctcgatgaag tggaaaaagc
    17941 gcacccggat gtgctcaacc tgttctacca ggcgttcgat aagggcgaaa tggcagacgg
    18001 tgaaggccgc ctgattgact gcaaaaatat cgtcttcttc ctgacgtcca acctcggcta
    18061 ccaggtaata gtcgagcatg ccgatgaccc ggaaaccatg caggaagtac tgtatccggt
    18121 gctggccgac ttcttcaaac ctgccctgct ggcgcgtatg gaagtggtgc cgtacctgcc
    18181 gctgtcgaaa gagacgctcg ccaccatcat tgccgggaaa ctggcccgcc tggataacgt
    18241 gctgcgcagc cgctttggcg cggaggtgat tatagaaccg gaagtgacgg acgaaatcat
    18301 gagccgcgtc acccgcgcgg aaaacggcgc gaggatgctg gaatcggtca tcgacggcaa
    18361 tatgctgccg ccgctctccc tgctgctgtt gcagaaaatg gcggcgaata cggcggttgc
    18421 ccggattcgg ttgtcggcag tggacggcgc atttacggca gacgtggaag atgctcagaa
    18481 cgacgagtcc gtcacaaagg atgaaacggt tttatgagca ggttgcgcgt tttccggcag
    18541 acaggatggt ttatcgcggg cctgatgacg ggcttacccg ccactgccgc gcccgcagaa
    18601 gcatcgtcaa tggccggtgt ggcggtcgcc gtcgccacca cgaccccgcc ggatgcgaca
    18661 gccacgttac aggccatgca gtcctgtcgg cgggagtccg cggcgctgga gcggcttgac
    18721 tgctatgacc gcctgctggc accgctgtcc ccgtcagggt ttgacggggc gctggtcaaa
    18781 gccggttttg tgggcgaggc ctggacccgc gccacagagc aggaaaaacg ccgcgagggc
    18841 aacaccacag aactgctggt gacgcaggtg ccgggggagc gcccgacggt ggtgataacc
    18901 accccggcta tcggtcatgt tccgccacgc ccggtgctga tgttcagttg cgtggacaat
    18961 atcacccgga tgcaggtggc gctgatgcac cctctggatg tccatgatat cgccgtcact
    19021 ctgaatgccg acaaccgggc attgcgcagc cactggtttg tgcgcgagaa cggcaccctg
    19081 ctggagtcca gccgggggtt atccgggatt gatgaaatta agcaactgtt cggcgcaaaa
    19141 acgctgacgg tggataccgg cgcagacaat gccgccggaa agctgacttt taacattgac
    19201 gggctggcaa gggcgattgc tcccctgcgc gatgcctgcc actgggcggg agaataaaaa
    19261 tatggcaatg gacttacgcg atccgaatgt ctggatatcg cacctgctgg aaaacctgcc
    19321 ggaagagaaa ctggcatcgg cgctgaaaga tgacaacccg aactgggagt atatcgacgg
    19381 cgaaatcgtc aagctgggat cgctggccca cagtcaactg gatatcccgg aactccagcg
    19441 ccgtgggctg gtcattctgg cttcggagag taaggatttt cgtctgctgg cgcatctgct
    19501 gagaaccctg caacacgccg gtgatcctca tctcgcattg cgcctgctgg cgctgtatgt
    19561 ggagcattac tgggcggtgg ctgcgccgca gaatatggcg cataaaaaac gctttgccag
    19621 ccaggtcatt aaacgctttg aaacaggtat tgaagttttt tcacagaacg ccgccacagc
    19681 gcagcgcgat gcgctgtcag gggaactggc aaaactggcg caatgctggc agtcacataa
    19741 cgccccggaa ctggcacagg ctactgatga tctttttgcc ctgtaccagc gtgcgtttaa
    19801 tcgtgcggca ccggctcctg tccccactcc ggcggcctcc ggtagttcac cacagaccac
    19861 cgccacgtct gaaagcggcg tgacgcaacc cagtgctccg gctccccaaa tcgtcatcga
    19921 cagtcacgac gacaaagcct ggcgcgacac gctgttaaaa gtggcggcca ttttatgtga
    19981 acgccagccg gattcaccgc agggctaccg tctgcgccgc catgcgcttt ggcagaacat
    20041 caccagtacg ccccaggcgg aaagcgatgg ccgtactcct ctggctgcgg tctccgccga
    20101 tatggtggca gattaccatg ctcagctcgg tagtgccgat atggcgctgt ggcatcaggt
    20161 ggagaaaagc gtactgctgg ctccttactg gctggatggt cactgccttt ctgcacagac
    20221 ggcgctgcgt ctgggttaca aacaggtggc agacgccatc cgcgatgagg tcatccgctt
    20281 ccttgagcgt ctgccccagc ttaccgggct gctgtttaat gaccacacac cgtttatcag
    20341 tgagcagacg aaacaatggc tggcagcttc gcccgacgcc aaagttgcac cggttgcgca
    20401 aatcggtgag gaatcgaagg cagccagagc ctgttttgct gagcagggtc tggaggcggc
    20461 gctgcgatat ctggacatgc tacccgaagg cgatccccgc gatcagtttc accgccagta
    20521 ccttgccgca cagttgacgg aagaggcggg gctggtacag cttgcgcagc agcagtaccg
    20581 gatgttgttc aggatgggat tgcagatgat ggtggcagac agggagccgt ccctgcttga
    20641 gcagctagaa caaaaattca cggcagaaca ataaaagaga acaggagttt ttgtgttcag
    20701 attaccaaca ccccgactac tcagcggact caaatcagcc ctgcgaccgg cgatgccccg
    20761 gtttaaagtc tctgctttct ggctgctgat actggcgtgg atttttctgc tggtgtggat
    20821 ctggtggaaa ggcccgatgt ggacgctgta tgaagaacag tggctcaaac cactggcgaa
    20881 ccgctggctg gcaacggcgg cgtgggggat tattgccctg gtgtggctta ccgtccgggt
    20941 gatgaagcgc ctgcaacagc ttgaaaaaat gcaaaagcaa cagcgcgagg aagccgttga
    21001 tccgctcagc gtggaactga acgcccagca gcgttatctt gaccgctggc tgctgcgcct
    21061 gcaacgccat ctcgacaacc gccgtttcct gtggcagttg ccgtggtata tggtcatcgg
    21121 cccggcgggc agtggcaaaa cgacactgct gcgcgagggg tttccgtccg acattattta
    21181 tgccccggag ggcgcacgcg gcgcagaaca acgcctgtac ctcacgcccc atgtcggtaa
    21241 acaggcggtg atctttgata tcgacggcac actctgcgct cccgctgatg cggatatcct
    21301 gcatcgccgt ctgtgggaac atgccctcgg ctggctgaag gaaaagcgcg cacgcagccg
    21361 ctgaacggga ttattctgac actcgattta cccgatctgc tgaccgcaga caaacgccgc
    21421 cgcgagcatc tgttacagac gctgcgcagc cgcttacagg atatacgcca gcatcttcac
    21481 tgccagttac cggtttacgt ggtactgacc cggcttgatt tattacaggg ttttgccgcc
    21541 ctgttccagt cactcaacag acaggatcgc gatgcgattc tgggcgtcac gttcacccgc
    21601 cgcgcccatg aaaatgatga ctggcgaaca gagttgaatg ctttctggca gacatgggtg
    21661 gatcgaatga atctggcgtt gccggatctg atggtcgctc agactcacac ccgcgcgtct
    21721 ttattcagtt tttcccgcca gatgcaggga agccgtgaac cgctggtgtc actgcttgag
    21781 ggtctgcttg atggcgaaaa tatgaacgtg atgctgcgtg gtgtctatct cacctcttcg
    21841 cttcagcgtg ggcagatgga tgatatattc acccagtctg ccgcccgcca gtaccggctg
    21901 ggcaataacc cactggcgtc ctggcccctg gtggacaccg cgccttattt cacccgcagc
    21961 ctgttcccgc aggcattact cgcagagcct aatctggcaa cagagagccg cgcctggctg
    22021 atacgttccc gtcgccgcct gacggttttc tctgccacag gcggcgtggc agcactgctg
    22081 ctcatcaccg gctggcatca ctattacaac ggtaactatc agtccggcat caccgtgctt
    22141 aagcaggcca aagcctttat ggacgtgccg cctccgcagg gggaagatga ctttggcaac
    22201 ctgcaactgc cgctcctgaa cccggtacgc gatgccacac tggcctatgg cgactggggc
    22261 gaccgcagcc gtctggccga tatgggactg taccagggac gacgtatcgg gccttatgtg
    22321 gaacagacct atctgcaact gctggagcaa cgttacctgc cctcgctgtt taacgggctg
    22381 gtcaaagcga tgaacgccgc gccgccggag agtgaagaaa aactcgcggt gctgcgcgta
    22441 atgcgaatgc tggaggacaa aagcggacgt aacaatgagg tggtgaagca gtatatggca
    22501 aaacgctgga gcgaaaaatt ccacggccag cgcgatatcc aggcacaact gatgtcccat
    22561 cttgactacg cgctggctca tactgactgg catgcagagc gtcaggcggg cgacggtgac
    22621 gccatcagcc gctggacgcc atatgacaag cccgtggaat cagcacagaa agaactgagc
    22681 aaactgcctg tctaccagcg ggtttaccag agcctgaaaa cgcgggcgct gggcgttctt
    22741 cctgccgacc tcaatctgcg tgaccaggta gggccaacct ttgaccaggt gtttacatct
    22801 gccgatgaca acaaactggt tgttccacag tttatcaccc gctacggcct gcaaagctat
    22861 tttgtaaaac agcgcgatga actggttgaa ctgacagcga tggattcctg ggtacttaac
    22921 ctcacccgca atgtgaaata cagtgacgcc gaccgtgcgg aaatccagca ccagttgacc
    22981 gagcagtata tcagcgacta caccgccacc tggcgggctg ggatggacaa tctgaatatc
    23041 cgcaattttg agtccatcgg acaactgacc ggggcgctgg agcaggttat cagcggcgac
    23101 cagcctttgc agcgggcgct gaccgtgctg cgtgacaaca cacagccagg cgtcttttct
    23161 gaaaaactct ctgccaaaga acgggaggaa gccctggcag agccggatta ccagttactc
    23221 acccgcctcg ggcatgaatt cgccccggaa aacagtaccc tggcagtaca gaaagacaaa
    23281 gaaagcacga tgcaggccgt gtatcagcaa ctcaccgagt tgcaccgcta cctgctggca
    23341 atccagaacg cgcctgtacc agggaaatcg gcgctgaaag ccgtgcagtt acggcttgat
    23401 cagaacagca gcgatccgat attcgccacc cgccagatgg caaaaacgct gcctgctccg
    23461 ctcaaccgct gggttggcag gctggctgac caggcctggc atgtggtgat ggtggaggct
    23521 gttcattata tggaagtgga ctggcgcgac agcgtggtga aaccgtttaa cgagcaactg
    23581 gcaaataact atccgtttaa tccgcgttct gcacaggatg cctcactgga tgccttcgaa
    23641 cgcttcttta aaccggatgg catactggat accttctacc agcagaacct gaagctgttt
    23701 atcgataatg acctgagtct ggaggatggc gataacaacg tcattattcg cgaagatatt
    23761 attgcgcaac tggaaactgc gcagaaaatc cgtgacatct tcttcagcaa acagaacggt
    23821 ctgggaacat cctttgccgt ggaaacggta tcgctttcag gcaataaacg ccgcagtgta
    23881 ctgaaccttg acggtcagtt agtcgattac agccagggcc gtaactatac cgcccatctg
    23941 gtctggccta acaacatgcg cgaaggcaac gaaagtaagc tgacgctcat cggcaccagc
    24001 ggcaacgcgc cgcgcagtat cagcttcagc gggccgtggg cgcagttccg cctgttcggg
    24061 gccggacaac tgaccggagt acaggatggc aactttaccg tgcgctttag cgtggacggt
    24121 ggcgcgatga cctaccgtgt gcataccgac acggaagata acccgttcag cggtgggttg
    24181 ttcagccagt ttggtctgtc agacacactg tactgaggag gcagggatga atagtaacgt
    24241 actgacacaa actatcgtta ccggcagtga cccgcgcggg ctgccggaat tcagcgccat
    24301 ccgcgaggaa ataaacaaag ccagccaccc gtcacagcct gagctgaact ggaaactggt
    24361 ggagtcgctg gcgctggcga tttttaaagc caacggtgtg gatttacaca ccgccaccta
    24421 ctatacgctt gcccggacac ggacacaggg actggcggga ttctgcgaag gtgcggaact
    24481 gctggcggca atggtaagcc acgactggga taagttctgg ccgcagggcg gcccggcgcg
    24541 tactgaaatg ctggactggt ttaactcccg aaccggcaat attctgcgtc agcaaatctc
    24601 ctttgcggaa tccgacctac cactgatcta ccgcacagag cgggcattgc agcttatctg
    24661 cgataagctc cagcaggtgg aactgaagcg cgttccgcgc gtggagaatc tgctctattt
    24721 tatgcagaac acgcgtaaac ggcttggacc ccagctgaag agtaacactg agaacgccgc
    24781 acagaccacg gtcagaacgc tgatttatgc cccggaaaca caggcatctt ccacaccaga
    24841 agcggtagtg cctcccctgc ccggcctgcc tgagatgaaa gtggaagttc gcagtctgac
    24901 agagaatccc ccacaggcca gtgtgataaa tcaaggcagt acggtaagag ggtttatcgc
    24961 agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg tggtggcagg tctatccggt
    25021 gcagcagcaa ctgttacagg ttaacgacac cgctcagggc gcagcaacgg tgtggatggc
    25081 ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa cttcttgata cctccccggt
    25141 acagccgctg gaaaccggga tgcagatgat gcgtgttgcc gacagtcgct ggccggaaag
    25201 cctgcaacag caacaggcct cgacacaatg gaatgaggca ctcaaaaccc gcgcacagag
    25261 tagcccgcag ttgcgtggct ggttgcagac ccgccaggac ttacatgctt ttgcagatct
    25321 agtgatgcag cgcgagaaag agggactaac cctttcctat atcaaaaatg tcatctggca
    25381 ggcggagcgg ggactggggc aggaaacacc cgttgagtct ctgttgacgc agtaccatga
    25441 tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa caaattaatg agcgactcga
    25501 aggcgtgtta agccgctggc tgctgctgaa gaataacgtc atgccagagg cggcaaccgg
    25561 taccacggcc gaaaaataat tatttaatta aaggagatta tcatggcaaa tataagttat
    25621 ttatctttga gtggagagac acaagggcta atttcagcag gttgctcaac gcttgattct
    25681 gtcggaaaca aagcgcagcc agaacataaa gatcagatca tggtttatgc gttaatgcat
    25741 tccatctcaa gaagtcaaaa tgtcaatcat catgaattaa ttatcaccaa accggttgat
    25801 aaatcctcac cattgctggc aaaagcactt agtgacaatg aaaaaatggc aatatgtgaa
    25861 tttatactct accgaaccag taaagcgggt atttaccagc cttattataa aataaatctg
    25921 tcaaaagccc gaatatcaag tattgatttt gtcacaccgc atgctgtcct ggaaaaagaa
    25981 ctggaaccac aggagagaat tgcctttatt tatgaagata tttcctggga gcatacgtta
    26041 gccggtacga atgcaatgag taaatggcag gatcgtgtac agtaacttac tgtaataaca
    26101 taataaatta ctattgcgga gagataaaat ggctggttta aggaataaca acaacacaca
    26161 gaatgcacag tgggctgatt atgtgggtga cattcttcgg ggggctcagc cgataaacca
    26221 gctagtacct cagcaccctt atcttaacga cgtcccactc attgatgagt taaggcatca
    26281 gaacactcat catgttattc tcttgacact tgatgttgca aaaaaaatac tcagcccaat
    26341 aacatcattt gattacattc attttatcac tacacatcct tcaggtataa aagatacact
    26401 cgcctggtta gttaacgcgg gtaaattaat gactgagttt gatgacaatg ggaaaattat
    26461 atttaacctt aatgccttaa agtatacaaa agcaagttac tttgaaatac ttggagagaa
    26521 atatataaaa atcacaacat ccagcccctg gcttttagaa aagctcggta aatatatttt
    26581 ctcttcacga gcgcctcaag ttctggaact cgctattggc tggcgaggag ctttatctga
    26641 atcaataaaa ggagttaagt tctgtatctg gttttcagta gcctggagaa ccatagaatt
    26701 tattatgagt tcagagcgag atctggttaa ttttcttggc gatttttcaa tggatgtggc
    26761 caaagcagtt atagccggtg gtgttgcaac cgctattgga agcctggctt cttttgcctg
    26821 tgttagcttt ggctttccag taattcttgt cggaggagca attttactga cagggatagt
    26881 gtgtacagtt gttttaaatg aaatcgatgc tcaatgccat ttatcagaaa aattaaaata
    26941 tgcaattaga gatggactaa aacggcaaca ggaacttgat aaatggaaaa gggaaaacat
    27001 gactccattt atgtatgttc
//
LOCUS       DAZGTR010000059        26672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGTR010000059 DAZGTR010000000
VERSION     DAZGTR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.059"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..443)
                     /locus_tag="ACHLCY_002674"
                     /pseudo
     CDS             complement(1..443)
                     /locus_tag="ACHLCY_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(602..994)
                     /locus_tag="ACHLCY_002675"
     CDS             complement(602..994)
                     /locus_tag="ACHLCY_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0369903.1"
                     /translation="MRDIQQVLERWGAWAANNYEDVTWSPIATGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGKTFMALARKHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(1307..2443)
                     /locus_tag="ACHLCY_002676"
     CDS             complement(1307..2443)
                     /locus_tag="ACHLCY_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HFP0369904.1"
                     /translation="MYKIRLQKNITILFIFIVMLSFSYWLANSFGMKWGVLAIIGFFL
                     GFTLSFSRFGIVAGWRDMLTMSNSYYVRIHLITIALEVVLFTLCLSFSHAFFGGKMVG
                     NITAISIPFLVGAFVFGIGMQIAGACASGTLYCCGEGRPAFWFVLCCYGIGTLIGNHY
                     KPVLKPIFPGEIITTKDITGGLWSGMLLSLVFILILYLVFSYIEKKRTGKISKIITSG
                     GSFWKDNRFTVLSGGLIIAVLNSLVLAMHGSAWTITGAIYDTSLHTASFLGVLSDNSV
                     ITKPLFQNPMVGMFVGGLLGAAMATVLTGKRIKEPLTRGSIMAGITGGLLMGTGGAYA
                     SCNLGGFFDGTASGSLHGWLWMFMALAGSVIGINLRRFFICSRC"
     gene            complement(3179..3538)
                     /locus_tag="ACHLCY_002677"
     CDS             complement(3179..3538)
                     /locus_tag="ACHLCY_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0369905.1"
                     /translation="MLPNEGRNARQKPPDQKQQSGGMYAPSGQQRPERLKIWGLTRKK
                     KILYGARSTGVKQGEETEVNMDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQR
                     EHIARRREICLANFRML"
     gene            complement(3456..3650)
                     /locus_tag="ACHLCY_002678"
                     /pseudo
     CDS             complement(3456..3650)
                     /locus_tag="ACHLCY_002678"
                     /inference="COORDINATES: protein motif:HMM:NF017845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
     gene            3735..4121
                     /locus_tag="ACHLCY_002679"
     CDS             3735..4121
                     /locus_tag="ACHLCY_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369906.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVTAEIGSPPFDVSGVAYLLHGMD
                     SFNSRYQIESTRRTLEAMVRDGLLERINVYERREGAGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVEGQ"
     gene            complement(4568..4861)
                     /locus_tag="ACHLCY_002680"
     CDS             complement(4568..4861)
                     /locus_tag="ACHLCY_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0369907.1"
                     /translation="MMTDSVAQKLEERGLWRRAATRWAEVLLHAETDSEREEAARRRA
                     ICISKTRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALP"
     gene            complement(4881..5297)
                     /locus_tag="ACHLCY_002681"
     CDS             complement(4881..5297)
                     /locus_tag="ACHLCY_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP0369908.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKRQRKNRRRINRLAEYWPE
                     LFGAENIKPLKAGVIHDMLQDAKARELAIGHGVLRGALTSYIHTTRYLKAIVAGGARY
                     GLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            complement(5314..5763)
                     /locus_tag="ACHLCY_002682"
     CDS             complement(5314..5763)
                     /locus_tag="ACHLCY_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0369909.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGTRGNQPPAGGDDPFSDDIPF"
     gene            complement(5760..6209)
                     /locus_tag="ACHLCY_002683"
     CDS             complement(5760..6209)
                     /locus_tag="ACHLCY_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369910.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAGKIYEKYAANS
                     KRAARAITYHFMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            complement(6497..8323)
                     /locus_tag="ACHLCY_002684"
     CDS             complement(6497..8323)
                     /locus_tag="ACHLCY_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP0369911.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGRLA
                     GDNVPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAVAGVRDA
                     DYYPEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGA
                     SQRGEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQN
                     AIKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPP
                     AEGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSG
                     KSVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAI
                     TGGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENE
                     RDPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCG
                     YLMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMA
                     EYGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            complement(8320..8442)
                     /locus_tag="ACHLCY_002685"
     CDS             complement(8320..8442)
                     /locus_tag="ACHLCY_002685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369912.1"
                     /translation="MASKNTGLTASSKTHPEFMCGDIYRNEYGSHMTMISRGKK"
     gene            complement(8500..8691)
                     /locus_tag="ACHLCY_002686"
     CDS             complement(8500..8691)
                     /locus_tag="ACHLCY_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369913.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDDHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
     gene            complement(8736..8945)
                     /locus_tag="ACHLCY_002687"
     CDS             complement(8736..8945)
                     /locus_tag="ACHLCY_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369914.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGGMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            complement(8949..9134)
                     /locus_tag="ACHLCY_002688"
     CDS             complement(8949..9134)
                     /locus_tag="ACHLCY_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369915.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(9127..9297)
                     /locus_tag="ACHLCY_002689"
     CDS             complement(9127..9297)
                     /locus_tag="ACHLCY_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0369916.1"
                     /translation="MAVSARPYFVWRFMQCLTDSIAIFTVTAATEREARARLPHAHLI
                     FVARIRQEVTHA"
     gene            complement(9352..9435)
                     /locus_tag="ACHLCY_002690"
                     /pseudo
     CDS             complement(9352..9435)
                     /locus_tag="ACHLCY_002690"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9769..9957
                     /locus_tag="ACHLCY_002691"
     CDS             9769..9957
                     /locus_tag="ACHLCY_002691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369917.1"
                     /translation="MTNEELQEIALSILERQREKAQRQRRLLLNPLPLTRPTRFALED
                     FVEKYPRRLKATKNRPPG"
     gene            complement(10264..11112)
                     /locus_tag="ACHLCY_002692"
     CDS             complement(10264..11112)
                     /locus_tag="ACHLCY_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFP0369918.1"
                     /translation="MKRPNMPVQGQGFVQSEELSQQYFAAIIPVSPGIIGGYETGIVS
                     ARALHKALGVGRDFTTWIKGRIEEYGFKKGVDFEVVENLYFDSPKRGSQSVNIEQFDS
                     GWTTKRGGDRRSEDYLISTNMAKELAMVERTEQGRAIRQYFIKCEEELHKAAPERAAA
                     LRRELKARVTVASFFKPMCAALELYRAELGKETHQHHYTTESNMLARIVLGGMTAKQW
                     AQANGIKGKPRDSMSVMQLEHLSYLDQSNITLIELGMTYQQRKAELIRLSQRWLARRM
                     GEHEHA"
     gene            complement(11180..11395)
                     /locus_tag="ACHLCY_002693"
     CDS             complement(11180..11395)
                     /locus_tag="ACHLCY_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0369919.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
     gene            complement(11782..12432)
                     /locus_tag="ACHLCY_002694"
     CDS             complement(11782..12432)
                     /locus_tag="ACHLCY_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0369920.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALADLNPSMRIGDVPQDK
                     FEQVESARTHIARAIRLHSGVKTSKDEPYRAGDIFLASFPFMEAGTPEAIIKAVTDTI
                     DDLRGTKNWEEKALNLGGRDLVRYIKETSRSGRGQHRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDMEALMKEKGISPKGLAKSTFMQKISAALLAVSQAD"
     gene            complement(12429..13691)
                     /locus_tag="ACHLCY_002695"
     CDS             complement(12429..13691)
                     /locus_tag="ACHLCY_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0369921.1"
                     /translation="MPKLTDIQIRAWIKSGERFEGRSDGGGLYLRYRKEDQIPTWRFR
                     YKFAGKARAMVIGSYADYSLSKARETAKELSARVALGYDVAAEKQERKAEALAKIEAE
                     KKAVRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKAEDVKPRHIDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGVKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRENELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVHPVIEWLRELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFNERRD
                     ALAKWTTLLVSLERGEHYNVTPIRRAKS"
     gene            complement(13881..14744)
                     /gene="yicC"
                     /locus_tag="ACHLCY_002696"
     CDS             complement(13881..14744)
                     /gene="yicC"
                     /locus_tag="ACHLCY_002696"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP0369922.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            14871..15587
                     /gene="rph"
                     /locus_tag="ACHLCY_002697"
     CDS             14871..15587
                     /gene="rph"
                     /locus_tag="ACHLCY_002697"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP0369923.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            15653..16294
                     /gene="pyrE"
                     /locus_tag="ACHLCY_002698"
     CDS             15653..16294
                     /gene="pyrE"
                     /locus_tag="ACHLCY_002698"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP0369924.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(16331..16927)
                     /gene="slmA"
                     /locus_tag="ACHLCY_002699"
     CDS             complement(16331..16927)
                     /gene="slmA"
                     /locus_tag="ACHLCY_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP0369925.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(17034..17492)
                     /gene="dut"
                     /locus_tag="ACHLCY_002700"
     CDS             complement(17034..17492)
                     /gene="dut"
                     /locus_tag="ACHLCY_002700"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP0369926.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(17470..18690)
                     /gene="coaBC"
                     /locus_tag="ACHLCY_002701"
     CDS             complement(17470..18690)
                     /gene="coaBC"
                     /locus_tag="ACHLCY_002701"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP0369927.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKLKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIMTRYDEKNRR"
     gene            18862..19530
                     /gene="radC"
                     /locus_tag="ACHLCY_002702"
     CDS             18862..19530
                     /gene="radC"
                     /locus_tag="ACHLCY_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0369928.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPAMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            19747..19983
                     /gene="rpmB"
                     /locus_tag="ACHLCY_002703"
     CDS             19747..19983
                     /gene="rpmB"
                     /locus_tag="ACHLCY_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP0369929.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            20004..20171
                     /gene="rpmG"
                     /locus_tag="ACHLCY_002704"
     CDS             20004..20171
                     /gene="rpmG"
                     /locus_tag="ACHLCY_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP0369930.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            20269..21078
                     /gene="mutM"
                     /locus_tag="ACHLCY_002705"
     CDS             20269..21078
                     /gene="mutM"
                     /locus_tag="ACHLCY_002705"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP0369931.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(21117..21596)
                     /gene="coaD"
                     /locus_tag="ACHLCY_002706"
     CDS             complement(21117..21596)
                     /gene="coaD"
                     /locus_tag="ACHLCY_002706"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP0369932.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(21604..22881)
                     /gene="waaA"
                     /locus_tag="ACHLCY_002707"
     CDS             complement(21604..22881)
                     /gene="waaA"
                     /locus_tag="ACHLCY_002707"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP0369933.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            23294..24352
                     /gene="rfaQ"
                     /locus_tag="ACHLCY_002708"
     CDS             23294..24352
                     /gene="rfaQ"
                     /locus_tag="ACHLCY_002708"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP0369934.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            24349..25473
                     /gene="waaG"
                     /locus_tag="ACHLCY_002709"
     CDS             24349..25473
                     /gene="waaG"
                     /locus_tag="ACHLCY_002709"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0369935.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVCSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAPYIVEANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARYYADTQDLYSLPEKAADIITGGLDG"
     gene            25466..26263
                     /gene="rfaP"
                     /locus_tag="ACHLCY_002710"
     CDS             25466..26263
                     /gene="rfaP"
                     /locus_tag="ACHLCY_002710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP0369936.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMHGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            26306..>26672
                     /locus_tag="ACHLCY_002711"
     CDS             26306..>26672
                     /locus_tag="ACHLCY_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0369937.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKH"
BASE COUNT         6360 a         6716 c         6454 g         7142 t
ORIGIN      
        1 atccatccag tcatcggttc gtctgagaat gacgtgcaac tgcgcacgcg acacccggag
       61 acaacggctg actaagctta ctccccatcc ccgggcaata agggcgcgtg cgctatccac
      121 ttttttgccc gtccatattc aacggcttct ttgaggagtt cattttccat cgttttcttg
      181 ccgagcaggc gctggagttc tttaatctgc ttcatggcgg cagcaagttc agaggcagga
      241 acaacctgtt ctccggcggc cacagcagta agacttcctt cctggtattg cttacgccag
      301 agaaataact ggctggctgc cacaccatgt tgccgggcaa cgagggagac cgtcatcccc
      361 ggttcaaagc tctgctgaac aattgcgatc ttttcctgtg tggtacgccg tctgcgtttc
      421 tccgtcccta agacatcaat catctgttct ccaatgacta gtctaaaaac tagtattaag
      481 actatcactt atttaagtga tactggttgt ctggagattc agggggccag tctaatgatt
      541 gattggcaag tctgacttct ccgccgcagt ttcctcatga ctgcggtttt ttttatgcgc
      601 gttatgccgt cactgaatcg cgctcaacat accgatccat ctccagcttc acatccagca
      661 tcatcagcat gccttcaaca acaccttccg ctttctgtag ccgctttccg atatgtccat
      721 cagagcatcc atgcttacgt gccagcgcca taaacgtttt ccccaacacg taataatcaa
      781 ccagcaagtc atgcagatcg ctgttgttcc tgttaaggcg agccatacac ccgcatataa
      841 tcatcgcgtc atcgtcacaa cactgtggac gtgattttac tttttcgggg atcagtccct
      901 taaatccggt agcaatgggc gaccatgtaa catcctcata gttatttgcc gcccacgccc
      961 cccatcgttc cagaacctgc tgaatatcac gcatcaacaa tcaccttgcg aataattatt
     1021 ggcaacggta actccgtaca gtaactccgt acatccccgt aattcggggt atataccgga
     1081 cggaggttaa aatacgaaaa tttcgtaggt tgtgagtata tttcagatag tcggttgctc
     1141 agctaatttt tctagagtag gtagtactac gtcgcgtagt tactgttttt tgatagtacg
     1201 ctttttcagc atgcatcccc cttgtataca tttaaaacaa gggggcttta ctttctgata
     1261 atagaattct tcccacagtt tcctgtcagg attcaattat ttgtttttaa cagcgtgaac
     1321 aaataaaaaa tctccgaaga tttatcccaa ttacactgcc agccagagcc ataaacatcc
     1381 acaaccatcc atgtaaactt cctgatgcag ttccatcgaa gaatcctccc aaattacagg
     1441 aagcatacgc tcctcctgtt cccataagca atccaccagt tattcctgcc attatgcttc
     1501 cacgagtcag aggctctttg atcctttttc ccgttagtac agtagccatt gcagccccca
     1561 gcaatccacc tacgaacatc ccaaccatag gattctgaaa taatggttta gtaataacag
     1621 aattatcaga taaaacccca agaaatgatg cagtatgcaa ggatgtatcg tagatggctc
     1681 cggtaattgt ccatgctgaa ccgtgcattg ccagaaccag tgagttcagt acagctataa
     1741 ttaacccgcc agaaagaaca gtaaatctgt tatctttcca gaaagaacca ccactggtga
     1801 taattttaga tattttcccg gtgcgttttt tctcaatgta tgaaaaaaca aggtatagta
     1861 tcaatataaa tacaagactt aataacatgc ctgaccacaa gccgccagta atatctttgg
     1921 tggtaataat ttctccggga aaaattggtt ttagcactgg cttatagtga ttgccaatta
     1981 atgtccctat accatagcag cacaaaacaa accaaaatgc cggacggccc tcaccacaac
     2041 aatagagtgt tcctgacgca catgctccgg caatttgcat tccaatacca aaaacaaacg
     2101 cacccaccag aaaagggata cttattgctg taatattccc aaccatcttc ccgccaaaaa
     2161 aagcatggct gaatgacagg cagagtgtaa acaaaaccac ctcaagagcg atggtaatta
     2221 agtgaatcct tacatagtat gagttgctca tggtcagcat gtctctccaa cctgcaacta
     2281 tcccaaatcg actaaaagac aatgtaaaac ccaagaaaaa accaataatt gcaagtacgc
     2341 cccacttcat accaaaacta ttagcaagcc agtaactaaa tgacagcatg acaataaaaa
     2401 taaataatat agttatgttt ttctgaagcc ttattttata catttttaat atacctatat
     2461 gtttccacgt gatgcgctga tgtacttgct gaaaaggaat cgttccagaa atgtatctac
     2521 gcaatatcct gaacacgtta cccaaaatgc ctgttccaga atgatactca cctataaata
     2581 accgtcaata tgacgctcaa gtactttctg tgagataatg cagcgaattg atactgacaa
     2641 ttcaacgtcc gtcatcgctc ccccaatgtg ttgaggttag gtagaaatga taacaaatac
     2701 accactcctg cccctgtaac ctcccctttt ttatacgctc atttttgcgc actttttcga
     2761 tcatcacgta tcggtttcgt tttgcgcttc ctgttgcgtt tcgtttttga gaaaaatgcg
     2821 cagttttttg cgcacttttt gaggtgtcta tccccctgca agccgcgtca tatctggctt
     2881 tgctctgttt gtctgtgtga tcgattttgt gatcagtttt atgatcgatt tgcgcacttt
     2941 tttgcgcatt ttttcaggga tttttcaccc catcgaagag gcttctaagg ggctgtgaag
     3001 tgggtagcat tttgctgtga tggaaggact accgctactg tctgataatg gtgacggagg
     3061 ccgctatgtt cggctctgat tgcagcctgc cgtatattca ccaatttacc tggctgttat
     3121 ttccataacc cgtaatgtcg ccgatcccgt ctgattccca tatcgtcaac agtcttattt
     3181 acagcatcct gaagttcgca aggcatattt cccgccgtct ggctatatgt tctcgctgcg
     3241 gatcggtgtg ggcctctttc attacgtcaa gccaacgagc ggcggctctt cgccatagtc
     3301 cctgagattc cagttgttcg gcttttttat cagttattgt gtccatatta acttccgtct
     3361 cttcgccctg tttcacccct gtacttcgtg ccccgtaaag gatcttcttt tttctggtca
     3421 atccccatat ctttaagcgt tctggtcgct gctgccctga cggcgcatac atgcccccac
     3481 tctgttgttt ttggtctggt ggcttttgcc gtgcattgcg cccttcgttg ggtaatatag
     3541 tgcctctctt catcagtttt tgcctgtctg aacaattctc cccagcgttc agcagcacgc
     3601 cgccagaggc cgctattctc cagcgcttca gccttgtcat cacgtatcat ctgtaccccg
     3661 ccgattctgt cctttcaaaa tttgtcttat ggcttattgt atgagggatt catcctacaa
     3721 caagaaaggt cactatgaga ctgacgcaat acaaaaaaga gatcctcagt tacttcactc
     3781 ccgacaatct ggcgtgggtt actgccgaaa ttggcagtcc gccgtttgat gtctccggtg
     3841 tggcttacct gctgcatggt atggattcat tcaacagccg gtatcagatc gaatcaacaa
     3901 ggcgcacgct ggaagctatg gtgcgtgatg gtcttcttga gcggattaat gtgtatgagc
     3961 gtcgtgaggg cgctggtgat ggtgttcgtc gaaaggtggc gcgttatggc ctgcctggtc
     4021 attctgttgt ggtgcgtgac ggtgagggcc ggaacggtgc aattgacggc gaatgtatac
     4081 gcatcaatgc tacagatgtg gtgctggtgg aggggcaata aaaaaccctg aaagatttct
     4141 ccgggtgctc cagttgcacg tggcagaaat ccttcagggt cagccgtaac gggcctctgt
     4201 acagagggct ccagttgccc agagatgagc acccgctaca gcctgttgtg ataagccgct
     4261 ttacagtgag ctccagttgc tcaaagaggc gcgtttttat cacaacctga ccgcgataac
     4321 ccgctgtaca gagggcttca attgcccaaa gaggcgcatt cttatcgcgg catgacgaaa
     4381 tcaccgtgat gttttcgtcg ttcactcatc ccgatgtgtg ggagatgcac cggaatgagt
     4441 gccgaaaagc cgctggctgg cttcatgcag tcggactcag ttgcccgggg cgaacaccat
     4501 caacggcctt ttggtggtga ttatagcgaa tcgcgataat tgagcaaata cggtgatgag
     4561 atgcgtatca cggtagcgct aaatcgtcgc ctgcatcact gaagctgtaa tttctccatt
     4621 catcctgcgg attaatcccc atctctttca gcgttctgtc tgcggccttt ttcacatcgc
     4681 caaaggtgac gaactgctca ggcatacgac gggttttact gatgcatatc gcccgacgtc
     4741 tggcagcttc ctcgcgttca ctgtccgttt ctgcgtgaag aagaacctca gcccatcggg
     4801 tcgccgcacg tcgccataaa ccgcgctcct caagtttctg tgccacgctg tccgtcatca
     4861 ctggctttac ctccggctga ttactgctga ctgtttttct gcttcatctg ctccagtctc
     4921 cgcttcagta gttcgctggc tactgattta tcttcttccg tgacttcacc gcaaggttgc
     4981 ccgttcagtc cataccgtgc gccgccagcg acaatggctt tcaggtatcg cgtcgtgtgg
     5041 atgtatgagg ttaatgcgcc acgtaatacg ccatgaccaa tcgccagttc ccgcgccttc
     5101 gcgtcctgca acatatcgtg tatcacgcca gccttaaggg gttttatatt ctctgcccca
     5161 aacagttcag gccagtactc agccagtcgg tttatcctcc tgcggttttt tcgctgccgc
     5221 tttgtcatgt gctgccacgg tatgcgccgt gatggtttac gtgtggtctg gtcctgttgt
     5281 gtggtctgct ggttcatgtt gtgctcctgt cattcagaac ggaatatcgt cagagaacgg
     5341 gtcatcacct cccgctggtg gctgattccc ccgtgttccg gtctgtcgtt ttgctctttc
     5401 cagtgcttcg gtcgccttcc cctgttgccc ccgcttcccg ccaggacgcg ccgaacgggc
     5461 actgattacg ctgtcagcga taacctgcca gccctgccgt atttctccgt tctgtccggt
     5521 ccactgactg atctgcatgt tacccgccac gctgataagg tcgccttttt catgtctcgc
     5581 cagcgcgtcg gcctgcttac caaatgccag gactgatagc cataacgtcg cctgtccgtc
     5641 ctgtgcctga ctgcatggca aagataccgc catacgcgcc agcgtcatcg gtgtgccctt
     5701 gctggtctgt tttacctgcg ggtcgtccac cagccgtccg taagctgcaa tctgtgctgt
     5761 catggtctgc gctccttgtt ccggtactct tggataatac ccattgtcat atcgaataaa
     5821 tcacatgccg ctttttctgc atcttccgct ttcaggtcat acagcgtcat gaaatggtaa
     5881 gtgatcgccc gtgctgctct tttgctgttt gctgcatatt tctcatagat ttttcctgcc
     5941 cgctcttcaa tatcccgtcg gaactcttca cgttcaaaca ggtcacggtg tatcaacatc
     6001 agcgaatcat tcagccatgt cacatcggcg tataccgttc gcttccagac aatcaggatc
     6061 ggaagtttat gtttgtcgca gtgatgcaga tgttcgtcag gtatataaag cgtggctctg
     6121 cctcgtaccg atacgaacct cacaacagat tccggttcgg ttaaatcgat agcgtgtgaa
     6181 tgtgattttt tggcgtgttt ctctgtcata aaatttttct caaaggaata aataaaaatt
     6241 tatgcccaaa aggtgttcaa ctgttcacct tttgctattt cgcctttaga ttcatgaagt
     6301 taaggggtga acgctttttg taaaggtgtt caccaactgt tcaccctgtt cacccgtgac
     6361 tgttcaaaaa tcatacaaaa aggtgaacag gtgaatactt tggtgaatag ttttacaaaa
     6421 agtgttcacc cattaacata ttgatactaa atacatttat tgcagggtga acagtggtga
     6481 acagtttttc tataacttaa tttttatcac cctcattttt cactggtgca catgacggca
     6541 gccagtcctc agactcatcc gtcagtatca cgtttgatcg cacaccttct ttagttctct
     6601 ttttcatata ctccctgcca tattccgcca ttgcgcctgg catatctgca ccgaagcgcg
     6661 tcagtgttac gggcttactg aaaccgtgcg ccctcatata cgagagatag gcgtgataaa
     6721 gataacggcg tggactgaaa ggaataatct cagcattacc gactaacagg ccgtcacaca
     6781 ttaccgacga catgagatag ccgcagaagt ccaccagcga atccccctca cgctttatcg
     6841 ccagtgcttc ttctgatttc tgctgctcat ataaaaggcg tctggcttcg tcctgatcag
     6901 caaaccgtgt aagcagatga cgaatcacta cagcaagctc accttctatt ttttccggca
     6961 gcatcggatc gcgttcgttt tccggtacaa cctccgaaaa attgaatatc acacgacgac
     7021 gtgagatccc cccgctgcgg tcactgaatg acatggcgct gttgttaacc gccagcacaa
     7081 ccgccggaat acgcgttgag tatggcgctt tgtgtttcgg gtcaattgcc accttgtcac
     7141 cgcccgtaat ggcctttatc cctgcaccat cacccgcgta gcgggtcata tccggcagaa
     7201 taatcagtga aaagccaacc actaatgcgc gttcccttgc atcctccagt gccttcatgc
     7261 ttgccgatac cgtgttggcc ttacctgcca gcatggtaca aatctccgcc ataacacttt
     7321 taccgcttcc accaggacct gttacctcaa tgaataactg ccagtcgtac cggttcgcca
     7381 gcaccataaa taacgctgcc agaacgcgat ctgccttgcg gtcattctca gccaccgaac
     7441 ggcgcaacca tttccagaaa ttcggcgcat gtgttgccag cgtttccccc tctgctggtg
     7501 ggctgaaagg taattcactg gcaattaaca accagtcgtt tttgttatgc tcccgaaaat
     7561 tacctgttct ggtatcaaat accccgttac tgaatccaat caggttacgg gctgtattcc
     7621 ccattacagg caaacttaac ttcatggtat cgaccgccga tttaatggcg ttctgcgaat
     7681 agctgatctc cgcatcaatg aaaatctgtg ccatagctcg ctgtaattct ttatcctgta
     7741 ctggctccca tacaacgccg ttgtaatggt gaacagtgtc agagtcagca tgaatcgcca
     7801 gttcaccgcc ataatgtgcc aggagaactt cgccgcgttg acttgctccc atctggttaa
     7861 gcgctagtga tgaagcgtta tcgtctttta cccgctcttt tttctttaca ggcagttcaa
     7921 ctaccttttt cttttccgcc tgctctgccc gttcacgttc cagatattcg cgccagttct
     7981 cccgtttctg gctgtgcatt ccttcagggt aataatcagc atccctgaca cctgccactg
     8041 ccagtttctg cccgatggta ttaacaagcc ccggacgcaa taaccccgcc tgatagagac
     8101 gcacgcgata gcgtccgtcc ggtacgattt gcaggttgtc cagttcggca agttgttgct
     8161 ctccaagcca gacaggtggc acgttatcgc cagccagtcg cccgtcctgt tcctgccact
     8221 gcttcgcatg tgcccacgca tcactgccag cgaaaataat cacttccgta tcggcgtgtt
     8281 ttttccctcg aggctgtttt tttaagttcg gtgcgctttt catttcttac ctctgctgat
     8341 catcgtcatg tggctaccgt attcattgcg gtaaatatcc ccgcacataa attcggggtg
     8401 agttttgcta ctggcagtta agccagtatt ttttgatgcc attgaattaa tctcctgaag
     8461 gagtcgtctt aatccttatt tcccgtttat ttttagccgt tattgttcgc cgcgtttttc
     8521 ttcctccgtc attaaccaca ccgccacatt gctggaaagt tgtttgataa gtcctgcaat
     8581 aacagaaagg tcatggtcgt ctatttcttc gtggttgcgt tcaatcatgc ggctgattac
     8641 ttcgacctgt tgcgcttgca gtgaggcttc tgccagtgtt atttgctgca ttactgcacc
     8701 cccttaattt catgatattc agaacaacaa aggattcagt cattgacgca tattgcatca
     8761 tcacccgatg cagcaatcag ccagctacct gccttacctg taagctgctt aatcagggcg
     8821 agcaatgact caatctcacc ctcagtcatt ccacctgtgt tacccatcat cagagaacaa
     8881 ataagttctg cctgcattgc gctttttgct gcttcatcaa cagtcatatc ggaaaaatca
     8941 cacatgaatt actcctcatc ctgaatacat gtaactgcgt ggataattac gccggataaa
     9001 ccggctttag ctgatttgtt aattactgtg ctgattgcag cctggacgtt ttcggccttc
     9061 acgttctgtg tgatgccgat ggtatggccg cgcttgttaa cagcacggga aaagacgcgg
     9121 aaggttttaa gcatgagtca cctcctgacg gatacgggcg acaaaaatca gatgtgcgtg
     9181 cggcagccgg gcgcgtgctt cgcgctcagt ggcagcggta acggtgaata ttgcaatgct
     9241 gtctgtcagg cactgcataa agcgccagac aaagtaaggg cgtgcggata cagccatgtg
     9301 aaaggctcct ctgactttgg gattataccc accgcccaag aggccaatct tgctggcggt
     9361 ggactgtacg gaattggcct tactggtgtt agaggaaacc agccttcccg aaggaagttc
     9421 catacagccc accattgttg aggtgtgcgt gtgcgccgat acaaaaaaaa agacgctggc
     9481 gcgtcgtgta tcgcctctaa ctccagcggg aggccaatcc cgatccccgt tttataaggg
     9541 atagcctcag tatatgccgc ttttttgctt aagtggtcgg atgtgttgcg agagaagttt
     9601 gtgcgaaaaa tacctgcgtt ttctttgaaa gcacataata acatcacgtt atttatgttt
     9661 atgcagtgtg tcgcaaagcg acctgaatca tcacgcaaat tacaaccata aaaaatacct
     9721 gcgctgtgat atgaggtact acagcgcacc gctttacggg tacagacaat gacaaatgaa
     9781 gaattacagg aaatcgcatt atccatactt gagcgacagc gcgaaaaagc acaacggcaa
     9841 aggaggttgt tacttaatcc actgccactc accaggccaa cacgatttgc ccttgaagat
     9901 tttgtcgaga aatacccacg gcggttaaaa gccactaaaa accgaccgcc tggctgatag
     9961 ccttgtccac cagctacact catggcatag ttggtgatgc gcttgatttc ctttatctcc
    10021 attggcgacc cttccgtggt cgcctttgtt ttgcctgcca ttcctgcatc atgcggatat
    10081 tcgcgccata cagggccacc agcgcgataa tttaataaaa cctccttctg ataatgctta
    10141 acgtgtttca tgcgctcagc ggcgaaatgg ctcacgatat ggtgcatgta atcagaatgg
    10201 ttgaggctca gttgtgcgaa cgtatccgcc gtgtcgatgt acagggcggc agcgttgcgg
    10261 atgtcaggca tgttcatgct ctcccatgcg acgggccagc cagcgctgtg aaaggcggat
    10321 taattcggct tttcgctgct ggtaagtcat acccaactca atcagcgtga tattgctctg
    10381 gtcaaggtag gaaaggtgtt caagctgcat aacgctcatg ctgtcacgcg gttttccttt
    10441 gatgccgttc gcctgcgccc attgtttcgc cgtcatgccg cccagcacga tacgcgccag
    10501 catatttgat tctgtggtgt agtggtgttg atgagtctct tttcccaact ctgcgcggta
    10561 caactccagc gcagcgcaca taggcttaaa aaaactggca acggtgacac gcgctttcag
    10621 ttcacggcgc aaagctgcgg cacgttccgg cgctgccttg tgtagctctt cttcacattt
    10681 gatgaagtac tggcgaattg cgcggccctg ttcagtgcgt tcgaccatcg ccagttcttt
    10741 agccatgttc gtggagatca aatagtcttc actgcggcga tcaccgccgc gctttgttgt
    10801 ccagccagaa tcaaactgtt caatatttac actttggctc ccccgtttcg gggaatcaaa
    10861 gtacaaattt tctacaacct caaaatccac acctttttta aatccgtact cctcaatacg
    10921 ccctttgatc caggtagtga aatcacggcc tacaccgagc gctttatgca gtgcgcgtgc
    10981 gctgacaatg cccgtttcat atccgccaat aatgccaggt gataccggaa taattgcggc
    11041 gaaatactgt tggctcagtt cttcggactg aacgaagccc tgcccctgta cgggcatatt
    11101 cggacgtttc attgaaaact cctgctgtcg aatcaggttt ggtgctgacg ggcgcggaac
    11161 tgtacgcccg tcatgctgat cactgcttgg tgttgttgcg gcgctcgttc agccagtggt
    11221 tgatctccac cgcattaaat acggtgtttt tctcccccag cttcgtgggc ttcggtaatt
    11281 tgccgatacg cacccagcgg tcgatagttg acacgctggt atcaagcaac ttagccaggt
    11341 tgcagcggcg gatgtagccc gtggtaggga tagcttcttg atggttagct gtcatgattc
    11401 cctcttgggt taaaagttga tgttttctct attctattgg ttgtttgtac agcattccat
    11461 caaatctgga atctatcaaa atattcacag attctattga aataatatat ttccagtaaa
    11521 agattgagtg agcgggaaga ggtgctaaga ctttgaaagt ttttgcttta ttggcgatag
    11581 gtcactgaat ccgtatacat cctgataccg tgatataccc aatgcgtcgt tatgcgctgt
    11641 tatggtttgc catcttttgc cagtttttaa cgtgttaatc ctgtttatac cgatttataa
    11701 cgattaataa cggtgaataa attttgctgg tgggggatgt atggggatta cagccccgtg
    11761 aatacagggc gtgttggctg gttaatctgc ctggcttact gccagtaatg cggctgatat
    11821 tttttgcata aacgtggatt tggctagccc ttttggcgat atccctttct ccttcatcag
    11881 cgcttccatg tcgcggtaaa tggctgaacg gtttggttct ccgtcctgaa tgtatgcggt
    11941 tgtgcctgat tttttcacga ggagtaaaac cagcaacccc atcatcttca tgtttccctg
    12001 ttcttcgtcg agtttcctgt gctgccccct tccgctgcgg cttgtttctt tgatgtaacg
    12061 gaccagatcc cgcccgccaa gattaagggc tttttcctcc cagttttttg tcccacgcaa
    12121 atcatcgata gtatctgtta ccgccttgat tattgcctct ggcgtgcctg cctccatgaa
    12181 cgggaaggaa gcaaggaata tatcccccgc tctgtatggc tcatccttgc ttgtttttac
    12241 tccactatgc agcctgatag cacgtgcgat atgagttctg gctgattcga cctgttcgaa
    12301 cttgtcctgc ggtacgtcgc ctatcctcat tgacggatta agatcagcta acgcaagggc
    12361 catttcgtgg ggagtgattg aaacagaagc gcaaaggcgc tctattggcg atttcttcag
    12421 gatgttaatc atgatttagc ccttctgatt ggtgtcacgt tgtagtgttc tccgcgttcc
    12481 agcgacacta acagagtggt ccacttcgcc agtgcgtccc gtcgttcatt gaaatactga
    12541 tagcggttgt atatgccttc gactcccttg atgcggtgat tgaggcatct ttcagcgact
    12601 accggatcaa caccaagcgc agctagatgt gtgcgtgctg tacggcggaa gtcgtgaata
    12661 gtgaaatttg gaacttccgg catctctgcg cggacttttg ccagcgcaac aggtagggtg
    12721 ctctcctgaa tgtgggggat catcctgtgc tgcattttcc gcgccggaag cacccaggca
    12781 ctattgcatg acaacacacg caactctcgc agccattcaa taacgggatg gacgagtgga
    12841 atatcgatgg ggtcgccgtt tttgctgcgt tcctccggca gatgccatac agcgttatca
    12901 aggtcgaatt cttcccatct ggcggcgcac aactccatct tcctgacaca aagcgccagc
    12961 aatagcttaa atgtgagttc attctcgcgg ctgaaaccct ttgcggtccg catagcctga
    13021 aagaatcgga ttagctcttc acgagtcagc caacggtcgc gggaggactc cttgccacca
    13081 gcatcagaga cttcaaacgc agagcatggg ttaatctcca gcacatgacg ttttaccccg
    13141 tagtcgaata tgcggcgcgt ccagcgtaga acgtccgttg caatggttgg cgctccacgg
    13201 tcaacaattg cctttagcat ctcgtcaatg tgtcgtggtt ttacgtcctc agccttcata
    13261 tgaccaatac gcgggtttat gtctttgtcg atgcgacggc gcaggatgtc gggatgtttc
    13321 catcgtggca gtatctgacg ttcgaaatat tcggcagcaa gctctgatac gcgcacggcc
    13381 tttttttctg cctcaatttt tgccagggct tcggccttac gctcctgttt ctctgctgct
    13441 acgtcgtacc caagtgcgac gcgagcggat agttcctttg ctgtttctct ggcttttgat
    13501 agcgagtaat cagcatagga accaatgacc attgcccgtg cttttcctgc gaatttgtag
    13561 cggaatcgcc atgttggtat ctggtcttct ttccggtaac gaaggtataa gccaccgcca
    13621 tctgaccgcc cttcgaatcg ttcacctgat tttatccatg ccctgatctg aatatctgtg
    13681 agctttggca ttaagaactc ctgaacattg aatagagagg tgtacccaaa cagatgttct
    13741 aaaatgcaat tgggtacacc tttgggtaca cgctaatcat gcgctttctt gcgtcgttat
    13801 gatagccatt aattacaaaa aaacccgcat tatcgcgggt ttgattgaat tattgcgctt
    13861 tattgattcc ctctgatagc ttactccaca ttttgaatct gctcgcgcat ctgctcaatc
    13921 aacactttca gctcaatcgc ggagtttgtc acttcggcat tgatagactt cgacgcaaga
    13981 gtgttcgact cgcggttgaa ttcctgcatc ataaagtcca gacgacgacc aacggcttct
    14041 tttttcttca gaatgttgta ggtctctttg acatgcgctt ccaggcggtc cagttcttcg
    14101 gcaacgtcaa ttcgttgtgc cagcagaacc aactcctgct ccaggcggtt gttttccagt
    14161 tgcacttgag catcttccag cttcgcgacc agacgctcac gctgccattg caggatttcc
    14221 ggcatatggg cgcggacttt gaccacttcg gcggtgacgc cttccagacg ctgctcgatc
    14281 aatgctttca gcgcctgacc ttcggtttca cgtgcgacga taaagtcgtc cagggtacca
    14341 tccaacgccg cgagaatttc agcggcaatc gcgtcgagat cctgctcctg ggctgccatt
    14401 acgcccggcc agcgcagaat atcaaccggg ttgatttccc cttcgtcact ctgcattttt
    14461 acccagttcg cggcagttac cagctgttta gccagttttt cgttgaggat cagctcacct
    14521 tgcgcgctaa catctggctc atagcgcagg gtacattcca ctttaccgcg cgtcaggcga
    14581 gaacgaatac gctcgcgaac gacaggttca aggctacgga actgctccgg cagacgaaag
    14641 taagtttcca gataacgctg gtttaccgag cgcatttccc aggttgcgct cccccattca
    14701 cccttgattt cacgccgggc gtaggcggtc atactgcgga tcatagacgt tcctgtttat
    14761 aaaaggagag gtggaaggat tatagccatc gatgccttgt caggatagga ataaccgccg
    14821 gaagtccgta taatgcgcag ccacatttgt ttcaagccgg agatttcaat atgcgtccag
    14881 caggccgtag caataatcag gtgcgtcccg ttaccctgac tcgtaactat acaaaacatg
    14941 cagaaggctc ggtgctggtc gaatttggcg ataccaaagt gttgtgtacc gcctctattg
    15001 aagaaggcgt gccgcgcttc ctgaaaggtc agggccaggg ctggatcacc gcagagtacg
    15061 gcatgctgcc acgttctacc cacacccgta acgctcgtga agcggcgaaa ggtaagcagg
    15121 gtggacgcac aatggaaatc cagcgtctga tcgcccgtgc tcttcgcgcg gcagtagatt
    15181 tgaaagcgct gggtgagttc accattacgc tggactgcga cgtgcttcag gctgatggtg
    15241 gcacgcgtac cgcgtcgatt acgggtgcct gcgtggcgct ggcagatgcg ctacagaagc
    15301 tggtggaaaa cggcaagctg aaaaccaacc cgatgaaagg gatggtagcc gcagtttctg
    15361 tcggtattgt aaacggtgaa gcaatttgcg atctggaata cgttgaagac tcagccgcag
    15421 agaccgacat gaacgtagtg atgaccgaag acgggcgcat cattgaagtg caggggacgg
    15481 cagaaggcga gccgttcacc catgaagagc tactcacctt gttggcgctg gcccgagggg
    15541 gaatcgaatc cattgtagcg acgcagaagg cggcgctggc aaactgattt ttaaggcgac
    15601 tgatgagtcg cctttttttt gtctgtagaa aagtaagatg aggagcgaag gcatgaaacc
    15661 atatcagcgc cagtttattg aatttgcgct tggcaagcag gtgttaaagt ttggcgagtt
    15721 tacgttgaag tccgggcgca aaagccccta tttcttcaac gccgggctgt ttaataccgg
    15781 gcgcgatctg gcactgttag gccgttttta cgctgaagcg ttggtggatt ccggccttga
    15841 gttcgatctg ctgtttggcc ctgcttacaa agggatcccg attgccacca ctaccgccgt
    15901 ggcgctggcg gagcatcatg accttgacct gccgtactgc tttaaccgca aagaggcgaa
    15961 agaccacggt gaaggcggca atctggttgg tagcgcgtta caaggacgcg taatgctggt
    16021 agatgatgtg atcaccgccg gaacggcgat tcgcgaatcg atggagatta ttcaggctaa
    16081 tggcgcgacg cttgctggcg tgttgatttc gcttgatcgt caggaacggg ggcgcggcga
    16141 gatttcggcg attcaggaag ttgagcgtga ttacaactgc aaagtgatct ctatcatcac
    16201 cctgaaagac ctgattgctt acctggaaga gaagccggaa atggcggaac atctggcggc
    16261 ggttaaggcc tatcgcgaag agtttggcgt ttaaagaaac tcgccggatg aaaagtcatc
    16321 cggcgtcata ttactgcaac tgtgccgcaa ttagcggcca gcgggcgtca aaatcatccg
    16381 tcgggcggta tttaaattcg ctgcggacaa aacgtgacag cataccttca cagaaggcca
    16441 ggatctggct tgccagcagg gtttcatcgg tggtgtaacc ttcaccctca cgcattctct
    16501 tttcacgcaa tacctggcgc agctgcgctt caatacgctc gaacagctgg ttgatgcgcc
    16561 cttgcaggcg atcctgttca aacattagcg catgaccagt gaggatgcgg gtcaggccag
    16621 gattacgctc accaaaaccg agaagcagca acacaatcag acgcaggcgc gctgtggtgt
    16681 ctttctcatc tttcagaatc aggttgatgc gagtaatcag gctatcttcg ataaactcaa
    16741 tcaggctatc gaacatgcgg gtcttactgg ggaagtggcg atacagtgcc gcttcggaaa
    16801 cgccgacaga ggcggccagt tttgccgtcg tgatacgttg gcttccatcg ctggattcca
    16861 gcatcagcgc cagagactga agtatttcct cgcgacggtt ccttttcgca gtttgttttt
    16921 ctgccatgtt acaaaatacc cctgaaaata agcacttgcc aggcggcacc cacgctatga
    16981 ccgcaaacga aatgtttgcg gctatgttat gacgttattc ggatgcgtat gtgttactga
    17041 cgaccagagt gaccaaagcc gccttcaccg cggtcggtgg cgtcgaaatc ttccaccaga
    17101 ttaaattcag cctgtactac cggaacaaaa atcatctggg cgatgcgttc gccaggttga
    17161 atggtgaagc tgtcctgacc acggttccac acggaaatca tcaactggcc ctgatagtca
    17221 gaatcgatca atcctaccag gttaccaagc acgataccgt gcttatgtcc caatccggag
    17281 cgcggcagca tcattgccgc cagtgaagga tcggcaatat gaatcgccag cccggtcgga
    17341 accagcgtag tgtcacccgg agccagttct acggcgtcgt cgagacaggc acgcaggtca
    17401 agtccggcag agccagaggt ggcataagtc gggagcggaa attccttccc aacgcgcggg
    17461 tccagaatct taacgtcgat ttttttcatc ataacgggtc atgatctcgt cgagtaataa
    17521 ttggccaagg agctctttgc gctcaagcgg taagacttta tctccgtcct gccagaaaag
    17581 gtgtaatgcg ttgttgtcgc tgttaaatcc ttgagttggc tgggaaacat cgttcgcgca
    17641 gatcagatca aggtttttac ggatacgttt ttgccgggcg tattcttcca cattatttgt
    17701 ttcggcggca aatccaacga cgtagggtcg atggtctttt agtgcggcaa cgcctgcgac
    17761 gatatcgggg tttttaacca tttttattgt taattcatca ccctgcgtgg cctgcttttt
    17821 gagtttctct ggggccacgg tagctgcgcg ataatccgcc acggcggcgc agccgataaa
    17881 aatattttgc tgctgtacag aagcattcac ggcggcttcc atttccagcg cagtcatcac
    17941 atcaacacgt ttaacaaacg gtggcgtcgg tagtgaaacc ggacctgata ccagcgtgac
    18001 gttcgcgcca cgacgggcag cggcggcggc gatagcaaaa cccatcttgc cggagctgtg
    18061 attagagata taacgcaccg gatcgagcgg ttcacgcgtc ggaccggcgg taatcataat
    18121 gttcagatgt ttcaggtcgt tgacgggcga aaaatgcgcc accgccatat ccacaatggt
    18181 taacggatcg agcattcgcc caggaccgat atcaccacaa gcctgactgc cactgtctgg
    18241 cccccagatg agcaaaccac gggaagcaag cacctctaaa ttatgctgcg tggcagcggc
    18301 acggtacatc tgctggttca tggcggggag cacggctaca ggcgcaggtg tagccagaca
    18361 aatcgtcgat accaggtcat tcgccattcc ggcagcaaca cgggcaatca aatctgccgt
    18421 ggcaggggcg agaatcacta aatcagccca tttacccagc tcaatatggc ccatagcggc
    18481 ttcggctgcc gggtccagca aactgtcgga aacgggataa ccagaaaccg cctgcaagct
    18541 aagtggggtg ataaaggctt ttgccgcttc ggtcatggct acgcggacgt cggccccgcg
    18601 atcgcgcaaa cgacgcacca gttcaggggt tttataggca gcaataccgc cgctaacgcc
    18661 gagaacgatt tttttaccgg ccaggctcat gatgattttt cctgttgggt gacaccagaa
    18721 gttggcgatt ttatcacaat acttttgttg tcgtgccttc acctgagaat cactttgcga
    18781 ggcgctttcc aggattgaaa actggccgtc gatttaacgg aacggctatg acaggatgcg
    18841 agcaccacaa aggaggtgaa ggtgaaaaac aatgcacagc tgttgatgcc gcgcgaaaaa
    18901 atgctgaagt ttggtattag cgccttaacg gatgtcgagc tgctggcgtt atttctgcgt
    18961 accggaacgc gcggtaaaga tgtattaacc ctggcaaaag agatgctgga gaatttcggc
    19021 tctctttatg gcttgttaac ctctgaatat gaacaattta gtggcgttca tggaattggc
    19081 gtagcgaaat ttgcccagtt aaaggggatt gctgaactgg cgcggcgtta ctacaacgtg
    19141 cggatgcgtg aagaaagccc tttactcagc ccggcgatga cgcgggaatt tttacaaagc
    19201 cagctcacgg gtgaggagcg ggagatcttt atggtgatct ttctcgactc acaacaccgg
    19261 gttataacgc atagccgtct tttttccggc acgctaaacc atgttgaagt ccatcctcgg
    19321 gaaattatcc gcgaagcgat aaaaataaac gcctcggcgc tgatccttgc acataatcac
    19381 ccttcgggtt gtgctgaacc cagtaaagcg gataaactca ttactgaacg gataataaag
    19441 agttgtcagt tcatggattt acgcgtgctc gaccatatcg tgattgggcg tggagagtat
    19501 gtttcttttg ccgaacgcgg ctggatttaa cccgctatgc gcgatccttc gggatctttg
    19561 tctgttcggg acttgagcac atcgctgagt cagcgtatac tacgccacct ttgagaatct
    19621 cgggtttggc atttgggcct ggcaatcgag agttcacata gaactgcgat gaccgggctg
    19681 taaagcctga cgaggcgcca ataccccata cgaagctcga gctaatttga tttttggaga
    19741 atagacatgt cccgagtctg ccaagttact ggcaagcgtc cggtgaccgg taacaaccgt
    19801 tcccacgcac tgaacgcgac taaacgccgt ttcctgccga acctgcactc tcaccgtttc
    19861 tgggttgaga gcgagaagcg ttttgtcacc ctgcgcgtat ctgctaaagg tatgcgtgta
    19921 atcgataaaa aaggcatcga tacagttctg gctgaactgc gtgcccgtgg cgaaaagtac
    19981 taagtactta gaggaaataa atcatggcta aaggtattcg tgagaaaatc aagctggttt
    20041 cttctgctgg tactggtcac ttctatacca ctacgaagaa caaacgtact aagccggaaa
    20101 aactggaact gaaaaaattc gatccagttg ttcgtcagca cgtgatctac aaagaagcga
    20161 aaatcaaata attctcgctt tgatgtaaca aaaaacctcg ctccggcggg gttttttgtt
    20221 atctgcttgc ccccatattg attgcatctg ttcattcctg gagatgctat gcctgaatta
    20281 cccgaagttg aaaccagccg tcgcggcata gaaccgcatc tcgttggtgc aaccattctt
    20341 catgcagtgg tgcgcaacgg acgcttgcgc tggccggttt cagaagagat ctaccgttta
    20401 agcgaccaac cagtgcttag cgtgcagcgg cgggctaaat atttgctgct ggagctgcct
    20461 gagggctgga ttatcattca tttggggatg tctggcagcc tgcgcatcct tccagaagaa
    20521 cttccccctg aaaagcatga ccatgtggat ttggtgatga gcaacggtaa agtgctgcgc
    20581 tacaccgatc cgcgccgctt tggtgcctgg ctgtggacca aagagctgga agggcataat
    20641 gtgctggccc atcttggacc ggagccgctc agcgacgatt tcaatggtga gtatctgcat
    20701 cagaagtgcg cgaagaaaaa aacggcgatt aaaccgtggc tgatggataa caagctggtg
    20761 gtaggggtag ggaatatcta tgccagcgaa tcactgtttg cggcggggat ccatccggat
    20821 cggctggcgt catcactgtc gctggcagag tgtgaattgt tagctcgggt gattaaagcg
    20881 gtgttgctgc gttcgattga gcagggcggt acaacgctga aagattttct gcaaagtgat
    20941 ggtaaaccgg gctatttcgc acaggaattg caggtttacg ggcgaaaagg cgagccgtgt
    21001 cgggtgtgcg gtacgccgat tgtggcgact aaacatgcgc agcgggcaac gttttattgt
    21061 cggcagtgcc agaagtaatt catgcgcgcc ggatggcata ccatccggca taaacgctac
    21121 gctaacttcg ccatcagcgc ctgatggaca ttctccggca ggaaatgggt gacatcgccc
    21181 tgatggcgcg ccacctcttt caccaacgat gaagagataa acgaccactc tttcgacggc
    21241 atcagaaaca cactttccag ttccggcatt aagtggcgat tcatatgcgc cagctgcatt
    21301 tcatattcaa aatccgccac cgcacgcagg ccacgaatca gcaccgtagc gtgttgatta
    21361 cgggcgaagt tcgccatcaa atcactaaac ccgaccactt ccacgttccc cagatgcgcg
    21421 gttgcctgct gtgccagcgc cacacgctct tccagggtaa acatcggttt tttactgggg
    21481 ctggcggcaa tcgccagaat aacgtgatcg aacatttgcg tggcgcgcgt cacgatatcg
    21541 atatgaccat tggtaatggg atcgaaagta cccggataaa tcgcccgttt ttgcataaca
    21601 acctcaatgc gttttcggtg gcaggtaagg ttccagcagt tgaagcagac gctgtagcgc
    21661 gccctggttt tgatacagta cttcaacggc atgacggcca tagaaactac ggtaatcggc
    21721 gtcggtgagt aaagaggaca cctcttttgc aagcgtagtg gcatcggtaa cggtaatcag
    21781 cccgcttgcc tgctccagcc gcgcgcaaat gtctttaaag ttaaaagtat gcggccccat
    21841 caataccgga atagcgtgtg cggcagcttc cagcggatta tgcccaccac gttcaaccag
    21901 tgaaccgcca acaaaggcga gatcggcaat gccgtacagt aacatcaact cgcccatcgt
    21961 atcgccaacc acaacctgcg tgctggtgga ggggacttcc cctgaagagc gtgtgatata
    22021 gcttagtcca gcctggcgga caagattaat cgcatccggg aagcgttctg gatgacgggg
    22081 taccaggatg agcaataaat tcgggaattg ctgtaacaat gcctgatgtg cggcgatcac
    22141 cacactctct tcgccttcgt gagtgctggt ggcaatccat accgggcggt gtggtgccca
    22201 ctggcggcgc agcgtcacgg ctttagcagc caactgcggc gttacagaaa tatcgaattt
    22261 caggctaccg gtaacggtca cctgattatt ttttgcgccc agcgccacaa aacgtgcacc
    22321 atcttcttca ttttgcgcag caatcagcgt aatacgacgc agcaagcgac ggacgaattt
    22381 acccagtttg gcataacctg cggccgagcg ggcagagagt cgcgcgttag cgatcaccag
    22441 cggaatttta cgtttgtgta gcgccgcaat caggttaggc catagttcgg tttccataat
    22501 caacaccagt ttggggtcga ctttattcag gaaacggttg agcgcatcgg gcaggtcata
    22561 cggcagataa acgtgctgaa catccttccc gaaagccgat tgtacgcgct ccgaaccggt
    22621 tggcgtcatg gttgtaacgg taatcggtaa atcaggataa cgatggcgca gcgcgcgcac
    22681 caacgggatc gccgccagag tttcaccgac ggagacggag tgcagcataa tgccgcctgg
    22741 ttttagcgga tggcggtaaa aaccgtaacg ttcaccccag cgttttcgat aggccggagc
    22801 cttacgtccg cgcacccaga gccgtatcca gatcagcggc tgaataaggt agagaagggc
    22861 ggtgtaaagc aattcgagca tagtaaatag ctgacttatg gatgtgctgg ggattctatg
    22921 tatttagctg tggctttacc attacttttc ccgtttttga cttaaatagc aatttacccg
    22981 ccaattcttg ctgaacatct atactttttt tgtattttta tgcgattcat tgatttctgc
    23041 tgtacgtgca ggaaggaatc gcagagcaac agtgaaagaa tatggttgct aatgatttta
    23101 tttgattgat ttctttattg aaaatcaaca atgtgcttaa aaatcattcg ggtcggattg
    23161 ccgcgaaaga agtgatacac tagcgcgtca atatcagtgc ggtatcagta ttcaggtagc
    23221 tgttgagcct ggggcggtag cgtgcttttt ctgcttcacc ttaatcggat aatctcaaca
    23281 aaaagagtta cttgtggata agccatttcg aagaattttg ctcattaaga tgcgttttca
    23341 tggggatatg ttattaacta ctcccgtcat tagttcgctg aaaaaaaatt accctgacgc
    23401 aaaaatcgat gtgctgcttt atcaggacac catcccgatc ctgtctgaaa atccagagat
    23461 taacgcgctc tacggcataa aaaataaaaa agcaaaagcc tcagaaaaaa ttgccaactt
    23521 ttttcatctc atcaaggtat tacgtgccaa taagtatgac cttatcgtca atctcaccga
    23581 tcaatggatg gttgctatac tggttcgctt attaaatgcc cgtgtgaaaa tttcccagga
    23641 ttatcatcat cggcagtctg ctttttggcg taaaagtttc acccatttgg tgccgttgca
    23701 gggtggaaat gtggtggaaa gtaacttatc cgtgctgacc ccattgggag ttgattcgtt
    23761 ggtgaagcag acaaccatga gttacccgcc tgcaagctgg aaacgtatgc gtcgcgaact
    23821 tgatcacgct ggtgttggac aaaattatgt ggttatccaa cctacggcgc ggcaaatctt
    23881 caaatgctgg gacaacgcca agttttccgc tgtgattgat gccttacatg ctcgtggtta
    23941 tgaagtcgtt ctgacgtccg gcccagataa agacgatctg gcctgcgtca atgaaattgc
    24001 gcagggatgc cagacgccac cagtaacggc gctggctgga aaggtgacct tcccggaact
    24061 tggtgcgtta atcgatcatg cgcagctgtt tattggcgtt gattccgcac cggcgcatat
    24121 tgccgctgca gttaatacgc cgctgatatc gctgtttggt gcgacagatc atattttctg
    24181 gcgtccctgg tcaaataaca tgattcaatt ctgggcggga gattaccggg aaatgccaac
    24241 gcgcgatcag cgtgaccgaa atgagatgta tctttcggtt attccggcgg cagatgtcat
    24301 tgctgctgtc gataaattac tgccctcctc cacgacaggt acgtcgttat gatcgttgct
    24361 ttttgtttat ataaatattt tccctttggc ggtttgcagc gtgattttat gcgtattgct
    24421 cagacagtcg ccgcccgagg tcatcatgtt cgggtttata cccagtcgtg ggaaggcgaa
    24481 tgccctgatg tatttgaact gatcaaagtg ccggttaaat cgcataccaa tcacgggcgc
    24541 aatgcggagt attttgcctg ggtgcaaaaa catttacgcg aacatcccgt cgataaagtc
    24601 gttggattca acaaaatgcc ggggctggac gtttattatg ccgctgatgt ttgttatgcc
    24661 gagaaagtag cgcaggaaaa aggctttttc tatcgcctga cgtcacgtta tcgccattat
    24721 gccgcctttg agcgggcaac cttcgaacag ggtaagccga cacagctgct gatgctgaca
    24781 gataagcaaa tcgccgattt ccagaaacat tatcagactg aagcggagcg ttttcatatt
    24841 ctgccaccgg ggatttatcc tgatcgtaaa tatagccagc agccagccaa tagccgtgaa
    24901 atcttccgta agaagaatgg aataaccgaa caacaatatt tattgttgca ggtcggttca
    24961 gacttcacgc gtaaaggtgt cgatcgttcc attgaagcac ttgcttcgtt accggattcg
    25021 ctgcgccaca acacattgct atatgttgtt gggcaggata aaccgcgaaa atttgaggcg
    25081 ctggcagaaa aacgcggcgt gtgcagtaat gttcactttt tctcggggcg caatgatgtc
    25141 tcggaattaa tggcggcggc ggatttatta ctgcatcctg cctaccagga agcggcggga
    25201 attgtgctgc tggaagcgat tactgcagga ttaccggtac taacaacagc cgtttgtggc
    25261 tatgcgcctt atattgtcga ggctaattgc ggcgaggcta ttgctgagcc attccgccag
    25321 gaaacattga atgagatttt acgcaaagcg ttaacgcaat cttcattgcg ccaggcctgg
    25381 gcggaaaatg cgcgatatta tgctgataca caagatttat acagtctgcc agagaaagcg
    25441 gcggatatca taacgggtgg tctggatggt tgaacttaaa gagccgtttg ccacgttatg
    25501 gcgcggcaaa gatccttttg aggaagttaa aaccttgcag ggtgaggtat ttcgtgaact
    25561 ggaaactcgc cgtactctgc gctttgaaat ggcgggcaaa agctattttc tcaaatggca
    25621 tcgcggcacg accctgaaag agataatcaa aaatttactc tcattgcgga tgccagtatt
    25681 aggcgcagac cgcgaatgga atgcgattca tcgactgcgg gatgtcggcg ttgatactat
    25741 gcatggggtg gcatttggcg aaaaaggcat gaatccgctg accagaactt catttattat
    25801 taccgaagat ctgacaccaa ccataagtct ggaagattac tgtgctgact gggcgactaa
    25861 ccctccagat gttcgcgtaa agcgtatgct tattaagcgt gtcgcgacga tggtgcgcga
    25921 tatgcatgct gcgggcatta accaccgtga ctgttatatc tgtcatttcc tgctgcactt
    25981 gcctttttcc ggtaaggaag aggagttaaa aatttcggta attgacctgc accgggcgca
    26041 gcttcgcacg cgcgttccac gtcgttggcg ggataaagat cttattgggc tttatttttc
    26101 ttcgatgaat atcggcctga ctcagcggga tatctggcgg tttatgaaag tgtattttgc
    26161 cgccccgctt aaagacattc tcaagcagga acaaggactg ctgtcgcaag cagaagcaaa
    26221 agccacaaaa atcagggaaa gaacgattcg aaaatcgttg taacccgtaa cttatttttg
    26281 ccaaaatttt ggatacagaa taaatatgtc tcaactcaat gatagtgaca tcatcctttt
    26341 tgagtataat tttcattatc aaaatataag atctaaaaat actcttgata tagcatttgg
    26401 tattgacaga aattttcttt ttggatgtgg tgtagccatc gcatctattc tattaaacaa
    26461 tagagaaatc tcttgtgaat ttcatgtttt cacagattat atcagtgata aagacaaatt
    26521 atatttttct gatttagcaa aacaatataa ttcaagaatt aatatttatg ttatcaattg
    26581 tgataagctg aagtcattac caagcacgaa aaactggact tacgcaacat attttcgatt
    26641 tataattgca gattatttct atcataaaca tg
//
LOCUS       DAZGTR010000060        26536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGTR010000060 DAZGTR010000000
VERSION     DAZGTR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.060"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..387
                     /gene="tnpA"
                     /locus_tag="ACHLCY_002712"
                     /pseudo
     CDS             1..387
                     /gene="tnpA"
                     /locus_tag="ACHLCY_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(409..597)
                     /locus_tag="ACHLCY_002713"
     CDS             complement(409..597)
                     /locus_tag="ACHLCY_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP0369938.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(601..960)
                     /gene="yeaR"
                     /locus_tag="ACHLCY_002714"
     CDS             complement(601..960)
                     /gene="yeaR"
                     /locus_tag="ACHLCY_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP0369939.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1133..1771)
                     /gene="leuE"
                     /locus_tag="ACHLCY_002715"
     CDS             complement(1133..1771)
                     /gene="leuE"
                     /locus_tag="ACHLCY_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP0369940.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(1898..2821)
                     /gene="dmlR"
                     /locus_tag="ACHLCY_002716"
     CDS             complement(1898..2821)
                     /gene="dmlR"
                     /locus_tag="ACHLCY_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP0369941.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            2924..4009
                     /gene="dmlA"
                     /locus_tag="ACHLCY_002717"
     CDS             2924..4009
                     /gene="dmlA"
                     /locus_tag="ACHLCY_002717"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP0369942.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            4260..5870
                     /gene="yeaV"
                     /locus_tag="ACHLCY_002718"
     CDS             4260..5870
                     /gene="yeaV"
                     /locus_tag="ACHLCY_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP0369943.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            5902..7026
                     /gene="yeaW"
                     /locus_tag="ACHLCY_002719"
     CDS             5902..7026
                     /gene="yeaW"
                     /locus_tag="ACHLCY_002719"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP0369944.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7082..8047
                     /gene="yeaX"
                     /locus_tag="ACHLCY_002720"
     CDS             7082..8047
                     /gene="yeaX"
                     /locus_tag="ACHLCY_002720"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP0369945.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMGDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8101..9216)
                     /gene="rnd"
                     /locus_tag="ACHLCY_002721"
     CDS             complement(8101..9216)
                     /gene="rnd"
                     /locus_tag="ACHLCY_002721"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP0369946.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLVCLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(9298..10983)
                     /gene="fadD"
                     /locus_tag="ACHLCY_002722"
     CDS             complement(9298..10983)
                     /gene="fadD"
                     /locus_tag="ACHLCY_002722"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP0369947.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEYQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11188..11769)
                     /gene="yeaY"
                     /locus_tag="ACHLCY_002723"
     CDS             complement(11188..11769)
                     /gene="yeaY"
                     /locus_tag="ACHLCY_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP0369948.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(11809..12504)
                     /gene="tsaB"
                     /locus_tag="ACHLCY_002724"
     CDS             complement(11809..12504)
                     /gene="tsaB"
                     /locus_tag="ACHLCY_002724"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP0369949.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(12562..14472)
                     /gene="yoaA"
                     /locus_tag="ACHLCY_002725"
     CDS             complement(12562..14472)
                     /gene="yoaA"
                     /locus_tag="ACHLCY_002725"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP0369950.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            14604..14948
                     /gene="yoaB"
                     /locus_tag="ACHLCY_002726"
     CDS             14604..14948
                     /gene="yoaB"
                     /locus_tag="ACHLCY_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0369951.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            15371..15670
                     /gene="yoaC"
                     /locus_tag="ACHLCY_002727"
     CDS             15371..15670
                     /gene="yoaC"
                     /locus_tag="ACHLCY_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP0369952.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(15790..15969)
                     /gene="yoaH"
                     /locus_tag="ACHLCY_002728"
     CDS             complement(15790..15969)
                     /gene="yoaH"
                     /locus_tag="ACHLCY_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP0369953.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16043..17404
                     /gene="pabB"
                     /locus_tag="ACHLCY_002729"
     CDS             16043..17404
                     /gene="pabB"
                     /locus_tag="ACHLCY_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP0369954.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETFVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            17408..17986
                     /gene="nudL"
                     /locus_tag="ACHLCY_002730"
     CDS             17408..17986
                     /gene="nudL"
                     /locus_tag="ACHLCY_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP0369955.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIIRRPQP
                     GVLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18170..19534
                     /gene="sdaA"
                     /locus_tag="ACHLCY_002731"
     CDS             18170..19534
                     /gene="sdaA"
                     /locus_tag="ACHLCY_002731"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0369956.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            19665..21263
                     /gene="pdeD"
                     /locus_tag="ACHLCY_002732"
     CDS             19665..21263
                     /gene="pdeD"
                     /locus_tag="ACHLCY_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0369957.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLLLEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(21267..22823)
                     /gene="yoaE"
                     /locus_tag="ACHLCY_002733"
     CDS             complement(21267..22823)
                     /gene="yoaE"
                     /locus_tag="ACHLCY_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP0369958.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(22811..22969)
                     /gene="yoaL"
                     /locus_tag="ACHLCY_002734"
     CDS             complement(22811..22969)
                     /gene="yoaL"
                     /locus_tag="ACHLCY_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP0369959.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            23286..24257
                     /gene="manX"
                     /locus_tag="ACHLCY_002735"
     CDS             23286..24257
                     /gene="manX"
                     /locus_tag="ACHLCY_002735"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP0369960.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAQAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            24320..25120
                     /gene="manY"
                     /locus_tag="ACHLCY_002736"
     CDS             24320..25120
                     /gene="manY"
                     /locus_tag="ACHLCY_002736"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP0369961.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25133..25984
                     /gene="manZ"
                     /locus_tag="ACHLCY_002737"
     CDS             25133..25984
                     /gene="manZ"
                     /locus_tag="ACHLCY_002737"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP0369962.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26039..26497
                     /gene="yobD"
                     /locus_tag="ACHLCY_002738"
     CDS             26039..26497
                     /gene="yobD"
                     /locus_tag="ACHLCY_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP0369963.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTSHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         6481 a         6866 c         6594 g         6595 t
ORIGIN      
        1 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga
       61 ctccagattt ttgaccatga cgcgacagaa aaactacgca cttacttttc aaatgtatgt
      121 gctgattttg aagctgaact tgttgaaatg gatggagaac cagatcacgt ccatttgtta
      181 attaactatc ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcgtatcg
      241 ggtaggttac tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt
      301 ttgtggagtc ctggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc
      361 caatacattg aacaacagca aacacctggt caggtggaaa accgcgcctt atatccccgc
      421 cctgaaggac ggggttttac ggcgcaccgg ataagaacgc acggtattca ccagatcttt
      481 tatcacttca gccgccactt ctggcaccag caaagtcatc ggcgtctctg tttcataatc
      541 gacagaaacg ccattgctgt tattggtgac ggtcacggta tacgttgctt tgcccatgat
      601 tcatttcccg ttatgaatga ctttccgttg ttgcgcacct tccatcagga cttcaggagc
      661 cacgaagaag tcaatgttga aataagtatc gtcagtcatg gcttcaatgt tgtgccactt
      721 ttctggaggg aacaccgcaa actgccccgc ttcgataagg atcacctgat caggctctgc
      781 actgtgttca tcagcgtagc cgagatattt gaccgcccca tgcataacgg aaaggcgtgg
      841 gtaaaccccc gggcgcgttc ctttatcaag atgacgttcg aatattccgg caggcgcagt
      901 ttgtttattc cagaaaggcg ttgagcgcgt atgaatataa ttctgtggga tttgcagcat
      961 cctattccct ccttcgggga atgcgctgaa aacggtttat tccagccgtt tcagggtacg
     1021 cctgataatt tgcattttaa ataccattta ttggttactt tttagcacca tatcagagaa
     1081 aaatcaggga ggattacaag tggggagccc gcgacaacgc gggctgaatg tatcaggatt
     1141 gcagcgtcgc cagtcgggcg gcgaaaccca cgaacatcaa accaatcagt gagttgccaa
     1201 ctttagccag tttctttttg gtacgtatgt actgcgtgac aaaagcacca gaaataatca
     1261 ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa
     1321 ttcccgtatg tggggcatta acatcgataa actgtacgaa aaacgacaca tagaacaaaa
     1381 tggctttcgg attagtcagg ctcaaaatta acgcgcgttt aaaaatggcc ccgtattggg
     1441 gctcatcgga tttggcctcg ttatttttac ccttcagggt cgcgtagaga attttactcc
     1501 ccagatagag caaataaaac gcaccaagat aacgtacaat gttgaataat atcggggtgg
     1561 tcttaattaa tgtcgccact ccagcccatg ccagaaacat caatactgca tcgccaataa
     1621 atacaccgca ggccgcaaga taaccgcctt tcataccgct actgacgcta tttttgagta
     1681 caaacagggt atttggccct ggcaccaaca caataaaaat ggccccaacc agataggtcc
     1741 agtaattcag aaccccgtat tcagcgaaca cattaacctc tttaattatc tttcgatcat
     1801 gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg
     1861 ttttaacccg atatcaaccc gataattgaa tcattaatca ggcatgctcc agtgaaaaat
     1921 tcgggtagtg ctcggcaaaa tactggcgta aaaattctac cgttatccgc actttcgctg
     1981 acgtcgccag ccttgaaaca taaacggccc agacgttcgc tggctggtaa tattccggta
     2041 gcacttgcac taaatgacca ctggcaatgt tttcgctaac atcccaccag gagcgcagag
     2101 caatcccctg cccatcgaga caccactgat ggacaatttc tccatgatta gacgacagtg
     2161 ggccggtgac tttaatcgca tggtggcctt ctttattgcg taattgccag acaccaaaag
     2221 gatgatcgcg ctctttaatg accaggcacg gcaatgcgga aagatcggtt aaatgtttcg
     2281 gcgcgccatg ttgcgcaata aattccggcg acgcgcagag aatgcggtaa ttagtcgcca
     2341 gtttgcgggc gattaaatta ggggcgatgt catcgccaat gcgaatatcg agatcaacac
     2401 cttcattgac caaatcgacc aaccggtctt ccacatcaaa acgtaattcc agttgcggat
     2461 acgccttagc cagcgctgat aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc
     2521 tgataatacg cagcatcccc tgcggcacct gacgcacgtc agaaagttcg tccatcatct
     2581 gaccgacatc ctgcaaaatc cgctgcgccc attcataaat ccgctctcct tcttcggtaa
     2641 tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg
     2701 cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac
     2761 cagcccgacg agccaccagc ataaagacgc gcaaatcatt cagcagcggt aaattattca
     2821 tgattcgtgt tttatgtttc accagttacg gggattaatt ccttttcagt caattatagg
     2881 atggtgatgt tgtcaatttt gatggtcagg aagtgagaac ccaatgatga aaacgatgcg
     2941 tattgctgcg atcccgggag acgggattgg caaagaagtc cttcctgaag ggattcgcgt
     3001 gttacaggct gccgctgagc gctggggctt cgccttgagt tttgagcaaa tggagtgggc
     3061 gagctgcgag tattacagcc atcacggtaa aatgatgccg gacgactggc atgagcaact
     3121 tagccgtttc gacgccatct attttggtgc cgtcggctgg ccggacaccg ttcctgacca
     3181 tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct
     3241 gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga
     3301 catcgatttt tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag
     3361 agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa tcggtattta cgcgtcgtgg
     3421 tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa agccgcccac gtaagacgct
     3481 aacttctgcc actaaatcaa acggtttagc catcagcatg ccgtactggg atgagcgagt
     3541 ggaagcaatg gccgagaatt acccggagat ccgctgggac aagcagcata ttgatattct
     3601 ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt ccaatttatt
     3661 tggcgatatc ctttccgatc ttggcccggc ctgcaccggc accattggca ttgccccatc
     3721 cgccaacctg aatccggaac gcactttccc gtcgctcttc gagccagtcc acggttccgc
     3781 gccggatatc tacgggaaaa acattgctaa ccctatcgcc acgatttggg ccggtgcaat
     3841 gatgctcgat tttctcggca atggcgatga gcgtttccag caagcgcata acggtattct
     3901 ggcagcaatt gaagaagtga ttgctcacgg gccgaaaaca cctgatatga aaggcaatgc
     3961 caccacgcca caggttgccg acgcgatttg caaaattatt ttgcgttaag gtcaaaccag
     4021 tttatttgaa ccgcgtcact gacgcggttt ttttattcgt tctttgcagt aaataacctg
     4081 cgtcatttca ccttttattg tttccgtttc gtgttttatg gctttccgta ttcttaattg
     4141 tttaatttat gtaacatgca aataatcaat ttgttaaaca caacatacaa ctcattaccc
     4201 tgcaaataat tacaactggc aaaagccaga gacgggcttt tatgcccagg agatgactca
     4261 tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag catcagcctg gtggccattc
     4321 ttttcatcgc agctgcatta agccttttcc cacaacaatc ggccgacgcg gccaacgcaa
     4381 tatacacttt tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa
     4441 tgggactggt gatttattta gccaccagta aatacggcaa tattcgtctt ggcgaaggaa
     4501 aaccggaata cagcacgctc tcctggctgt ttatgtttat ttgtgccggt ttaggttctt
     4561 ctacgcttta ttggggggtt gctgaatggg cctattatta tcaaacgcct ggattaaata
     4621 tcgcaccgcg ttcacaacag gcactcgaat ttagcgtccc ttactccttc ttccactggg
     4681 gcatcagcgc ctgggcaact tatacgctgg cctcattaat catggcttat cactttcatg
     4741 tgcgtaaaaa caaaggtctg agtctttccg gcattattgc cgcgattacc ggcgttcgcc
     4801 cgcaaggccc atggggaaaa ctggttgatt tgatgttcct gatcgccact gtcggcgcac
     4861 tgactatttc ccttgttgtc accgcagcaa cctttactcg tggactttcc gcgctgaccg
     4921 gtttacccga taacttcacc gtgcaggcat ttgtgatcct gctttccggc ggcatttttt
     4981 gcctaagctc gtggattggt atcaacaacg gtttgcaacg tctgagcaaa atggttggct
     5041 ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg cccaaccgaa tttattacca
     5101 acagcatcat caatgccatc ggtctgacca cgcaaaactt cctgcaaatg agcttattca
     5161 ccgatccgct tggcgatggt tcatttaccc gcaactggac cgttttctac tggctgtggt
     5221 ggatctcata cacccctggc gtagcaatgt ttgtcacccg cgtttcccgc ggtcgtaaga
     5281 ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg
     5341 gcgtaatgga aagctatgcc attcatcagt ttatcaatgg cgtaatcaac gtcccacagg
     5401 tgctggaaac actgggcggc gaaacagctg tacagcaagt tctgatgtcg ttgccagccg
     5461 gtaaattgtt cctcgccgca tacctgggcg tgatgattat tttccttgcc tcgcatatgg
     5521 atgcggtggc ctacaccatg gctgcgacca gtacgcgtaa tctccaggaa ggtgacgatc
     5581 ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac tctgatcccg ctttccatct
     5641 tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt cgtgctcaca gcccttccct
     5701 tcctcgtcat tttactggtg aaagtcggcg gatttattcg ctggctgaaa caggattacg
     5761 ccgacattcc ggctcatcaa gttgaacatt atctcccgca gacaccggtt gaagccctgg
     5821 aaaaaacgcc agtgctccct gcgggaaccg tattcaaagg cgacaactga gcgccatcat
     5881 cctaacgata aaggtatccc tatgagcaat ctgagccctg actttgtact acccgaaaat
     5941 ttttgcgcta acccgcaaga ggcgtggacc attcctgccc gtttttatac cgatcagaac
     6001 gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc
     6061 agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga tcattggcga aagcatcgtg
     6121 ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt
     6181 catcagttgt tgagcggtga aggaaaagca aaaaatgtga ttacctgccc gtatcacgca
     6241 tgggcattca aactcgatgg caatctggcc catgcacgta actgcgaaaa cgtcgccaat
     6301 ttcgatagcg acaaagcgca actggttccg gtgcgtctgg aagaatatgc cggattcgtc
     6361 ttcatcaaca tggaccccaa cgccaccagc gtagaagatc agttacccgg tctgggcgcg
     6421 aaagtgctgg aagcctgccc ggaagtccac gatctgaaac tggcggcccg ctttaccacc
     6481 cgcacgcctg ccaactggaa gaacattgtc gataactatc tcgagtgcta tcactgtggt
     6541 ccggcgcatc caggtttctc cgactcagta caggttgatc gttactggca caccatgcac
     6601 ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa
     6661 gagggtacgg atgcggcatt ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc
     6721 accccgatca aagggatgat gacggtcatt tatgaattcc cggtggattc tgaaactacc
     6781 ctgcaaaact acgatattta cttcaccaat gaagagttaa ccgacgagca aaaatcgctg
     6841 attgagtggt atcgcgatgt gttccgtccg gaagatttac gtctggttga aagcgtacag
     6901 aaagggctga aatcgcgtgg ctatcgtggt caggggcgca tcatggccga cagtagcggt
     6961 agtggcattt ccgaacatgg tatcgcccat ttccataatc tgctggcgca ggtgtttaag
     7021 gactaatgac atcggcggcg gtattttccg ccgctgggct gatttttgat ggagtacagc
     7081 aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca
     7141 ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg
     7201 aagtcacatc attgtgcaga tgggcgatgg tgataaccag tacagcaatg cgtattcact
     7261 actgagttcg ccgcatgaca cctcttgtta tcagattgcc gttcggctgg aggaaaactc
     7321 gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc
     7381 aacgcctaat aacctgtttg cgctaattcc ctcagccaga aagcatctgt ttatcgcggg
     7441 cggtattggt atcacccctt tcctgtcgca catggcagag ctgcaacaca gcgacgtcga
     7501 ctggcagcta cattactgct cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt
     7561 ccagcatccg caggctgaga aagttcactt gcatcactca tcaaccggaa cacgactgga
     7621 attagcgcga ttattggcgg atatcgaacc tggcacacac gtttatacct gtggtcccga
     7681 ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg gacatcgtcg ccgatacgct
     7741 gcactttgag caatttgcta tcgaagacaa aaccggcgat gcctttaccc tggtgcttgc
     7801 ccgttccgga aaagagtttg tggtgccgga agagatgact attttgcagg ttattgaaaa
     7861 taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac
     7921 agcaatactg gaaggtgaag ctgaccatcg ggatcagtat tttagcgatg aagagcgtgc
     7981 cagccagcaa agtatgttga tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga
     8041 tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt
     8101 ttactgcgga tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca
     8161 gccggaaatc agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag
     8221 ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg atcttatgcg tttcgctcac
     8281 gtcagtaatc agcgacttaa tcgctttaaa cactttacga taaccaggca tgtccatcag
     8341 gttaagcatc ggctgcggta aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc
     8401 cagcagcgtt ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc
     8461 gcctaaactt cccggcatat aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa
     8521 gttcaccgcc agatcacgct ctcgcgcctt gcgcagtcgc cagtcggcta acagttgcag
     8581 acagaccagt tggcgtgtgc gtaactgcca ggcattggtg atatcacgcc aggcatcttc
     8641 cggcgcaacg acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg
     8701 tagccagccg gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca
     8761 gacatccgcc gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt
     8821 gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc
     8881 ccatgacatc gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca agggttgtgg
     8941 taattcgcca aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt
     9001 tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg
     9061 atcaattagc gccagatgct cgccatcgaa aagttgaatc aaccccagct gcgggtaata
     9121 agtgcgcgta cgaacaaatt cagtatccag ggctatcgcc ggaaaggcac ggacggcttc
     9181 acacaaagaa gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgttttctct
     9241 ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacactca
     9301 ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc
     9361 gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcccgt
     9421 gagctgacgg cggcaaaagg tcaccagtga ctcttcggta agcgatggat cttttttcac
     9481 tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc
     9541 ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc
     9601 ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc aggaaccctt cttcatccat
     9661 caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatctg tagcatccgg
     9721 acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc
     9781 cggtggtact tcattatcat catcatccac cagtttggct tccgtcgacg gcaccggcaa
     9841 accgatgcta ccactatgat aatcaatatc atatgggtta acgctgacca gcggcgcaca
     9901 ctcggtaagg ccatagcctt ccagcagata ctgtcctgtc agtttcaccc aacgctctgc
     9961 caccacttgc tgcactggca tccctccgcc tgcggaaaga tgcagactgg agaaatccag
    10021 ctgctggaac tctttattgt tcagcaacgc attgaacaag gtgttaacgc ccgtgatagc
    10081 ggtaaacgga tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat
    10141 aagcaggttc tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat
    10201 gtgatacagc ggcagcgccg tcaccaccag ctctttgccc ggatgcagca gcggaccata
    10261 ggtcgcgtta acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt
    10321 cgccacacca gtggtgccgc cggtgtattg cagaaaagct aaatcttccg gcaccagttc
    10381 gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc
    10441 tggcagatgg tatttcggca ccaaacgctt gatgtattta acaacgaaat tgactaccgt
    10501 gccttttgcc gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt
    10561 tttatcaacc actttttcca gtgtgtgagc aaagttagac acgataacaa tcgccgatgc
    10621 gccgctatcg ttaagctgat actcaagctc acgcggggta tacaacgggt taacgtttac
    10681 gacgatcatc ccggcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg
    10741 catcatcaac gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc
    10801 aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt acctccccca tattcacaaa
    10861 cgcaggttga tcggcgtagc gcgcgaccga ctgctcaaac atatctacca gagattgata
    10921 acggtcaggg ttgatctccg tcggaacgtc cgcgggataa cggttaagcc aaaccttctt
    10981 caattcttca cctctaaaat gcgtgttcgt cgtcatcgca accccaaatg atatacatgc
    11041 cgttaacata atattaactc atcataccag cttgataatt acccaacgaa aaggttgcga
    11101 agcgcgtcac tatttatttt tatctttacc gtaagaatgc agaaacagcg gaccagccgc
    11161 tgtttctttt tctttaaaaa caagcaatta ttcagttaca actgtttgta ctctcgcggg
    11221 gccgggatta taccagcccc atccgccgta gccatagggc cagccacgac cgccataaaa
    11281 ccatggatca atcggctgag gcggcataat cacctgctgg gttaaatgcc aacgtttgta
    11341 acccgttact tgcatcacca taaatttata gggcgtattg ccgattttgc cgtcaaccgc
    11401 accagtgatt ggcccgacta ccgtaacaag ttgtccacgg aaatccaccg ggtccagaaa
    11461 accgttcaca tcggcataaa tgcgaccgcg agaaggttct cccagcgtcg gtctggctcc
    11521 gctgtccagc ggtacggtag caatttccag gcgggttttc ccttgctggt tttgtaccgc
    11581 aaccactttg ccaccaaagc gtgcctcctg accaacgtac agctgcggcg cactcatcac
    11641 ccgaactaaa tcttgttgcg gcgtgggact gctgccttta atggcgtccg gcacagtgac
    11701 acaaccgctc agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg
    11761 aaccgccatg atgcgactcc tttttttctc agggcatact cttaagattc attctttgcc
    11821 cggaagtttc ttccatgcga cgttgttacg taaataaacc ggttcggcat gttccaccgc
    11881 caccgtttta ccctcggcaa acatctgaca cgcaatcggc agcatatctt ccgccgctgg
    11941 cagtaaaact tctccgtcgc gcaaaaccag cccgctctct ttaccgagat ccggccaggc
    12001 ttgccagccc gtccctaccg ttacccattc gccggaaagc tgttgcattc gttcatgaac
    12061 gagttcgggt tttagtacgg cttcggtttc ttcaccgcgc cagataccgt tttcatcacg
    12121 ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca atggctgcca gcacgcgggt
    12181 tgcgccgttt ttgcgccacg ccccttgcgc catcgtcatt agcgtggaga cgccaatcat
    12241 cggtagctct gcgccaagtg ccagcccttg cgcaatgcca ataccaatgc gcacgccggt
    12301 aaagctaccg gggccgcgcc cgtaagccag agcgttaata tcagtcaggg aagttccgct
    12361 ggtggtcagg atatcctgca ccatcggtaa gattcgttga gtatgttcac gagggcaaag
    12421 ctcaaaatga gcgttgacag taccgtcgtt ccacagggcg acagagcagg cctctgtcgc
    12481 ggtatcgata gccagaattc gcatgggtct tcgtgcttag atcaataaaa aggcgcgcat
    12541 cataccatac tccgtaacaa attacctgga ggatggtatc gcaaggaaac gaaccgcacg
    12601 ggcaatgtca cgagtgcgtg gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg
    12661 acgcatcacc agccgattgt cacaaatcac cagcacgcca cgatcgtcgg cgtcgcgaat
    12721 cagtcgccct accccctgtt tgagagtaat gacggcatct ggtagttgca cttcatcgaa
    12781 cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct tttaacagtg gatcatccgg
    12841 cgaggtaaac ggcaacttgt cgataatcac caatgacaat gtatcgccac gcacgtccac
    12901 cccttcccag aaactactgg ttgccacaag aagcgcgtta ccggcgctga caaattgctg
    12961 caacagttgc cctttgctgg tttccccctg caacaaaacg ggaagcgtca tggtagcgcg
    13021 gaactgctcg gccagatcac gcatcatggc gtgcgaggta caaagcataa aacaacgacc
    13081 gttgttagct tcgatgatcg gtcgcagcat tgccgctaac tggcgagcag aacctggctg
    13141 gttggtttgc ggcagattgc gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg
    13201 gcttggcaat agcaacgact ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag
    13261 atcgtcgttc accgacagcg ttgctgaggt gaagatccag ctaccaggct tttgcgccat
    13321 taactctttg aatttatccg ccacgctgag cggcgtgaga gccagagtaa aatggcgcga
    13381 agtgcattcg taccagtagc tgtagcccgg ctgattgatc tcttttagcc gcttcagccg
    13441 tgtgcgatac aacgtagcgc gctcaaatgc cgcatccagc aaggcggaac gccccagtga
    13501 cagtttcgcc acgtcataac aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg
    13561 aatttgcgga ttagctaaca gctcacgcag gttaccgcga taacctggct caccgagttg
    13621 cagacgaaaa tcctgcgcac tctgggcaag acgatcagcg cacttttgta actgctgggt
    13681 gtcttttaat tcggtgcggt aagcgatggt gatgtctttt gccaggtcga gcagttgtcg
    13741 actggagagt gactgaccaa aatactggct ggcaatgtcc ggtagctggt gggcttcgtc
    13801 gaagatcatg acgtccgctt ccgggatcag ttcgccaaat ccactctctt taaccaccat
    13861 atccgccaga aagagatgat ggtttaccac caccacatcg gcgtccatcg cttttttacg
    13921 tgctttgacc acaaagcaat ctttatacat cgggcagtcg ctgccaagac agttgtcgtt
    13981 ggtgctggtg accagcggcc acgcctgtga atcttccgcc acgctgacgc aggtgctgat
    14041 atcaccatcg actgtttgat tagaccagga gcgcagcagg atcacatcgc ttaagatttg
    14101 taccggcaga tcgccccccg ccagcgcctg ctgttcgaga cgctcgaggc agaggtagtt
    14161 tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg agactgttgg
    14221 caaatcgcgg ctgtagagct gatcctgcaa cgcttttgag ccggtcgaga taatgacttt
    14281 ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt ttgcccgtac cggttcctgc
    14341 ttccaccacc agcggctggc ctttttctat cgcctgggtg acggctaccg ccatctgtcg
    14401 ctgtggttct cgcggcttaa agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa
    14461 atcgtccgtc acactacccc ctgttgattt gaacagggat tatgtcagga tgagggcgct
    14521 ttcgccagtt gaagtggtga cgacgacctc acattgtggc agtctttgca gcacgaaaat
    14581 ggaaggataa tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt
    14641 ctgatgtggt aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg
    14701 ccgatgcctt tgagcaaacc gccaacacgc tggcgcagat tgatgccgtg ctggaaaaac
    14761 agggcagcaa taaatcgagc attctggatg ccaccatttt cctggccgat aaaaacgact
    14821 tcgcggcgat gaataaagcg tgggatgcct gggttgtcgc gggtcatgcg ccggtgcgct
    14881 gcacggtaca agcgggtttg atgaacccga agtataaagt tgaaattaag attgtggctg
    14941 cggtgtaagc tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg
    15001 cttttacata aatcgaccag agattaagat attacctgtt gatgctcctg ctaattcatt
    15061 atttattttt ataatttcaa ttttatctat aaaactaact atataaataa ccataaccat
    15121 gccgttgtgt tcgccctctg actagcaact ctggttttct ttcttagctg aataatctgg
    15181 ttatagcgtg aggactgacc gttaaatttc tccatcttac tggataacac accatgagct
    15241 gctaccttta aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct
    15301 ttgatattag atgcaaatta aggtcatata tagtcttatc gataacatgg ttaattttaa
    15361 ggataagaat atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt
    15421 atcagcgcca acaccaagtt cgatgaatga aagcacggcg aagggaatct ttaaatattt
    15481 aaaagaactg ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg
    15541 ctggaaccct gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agacaggtga
    15601 acgctctgtg attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc
    15661 actggtctga gttaaattta tatcagcata aatgggtcag gacgctttta atcacatata
    15721 aaaagcgtcc tttttctcac aatcaaatga tcatcggctt ctataacgaa atcgatacgg
    15781 attagtgtgt tactcgtctt catcctcaaa acgcgccacg attcgctcgc cggagtggtt
    15841 ggcacgcagc tcttctgcca ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat
    15901 cagttcctgg atccgctcga cagctttttg ctgctgttca tgggtgagtg aaggtaaacc
    15961 tgcaaacatt gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac
    16021 atagccagta gagtcaggac tgatgaagac gttatctccc gctgtgatta ctttaccctg
    16081 gcgtcaggac gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct
    16141 tttacactcc ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc
    16201 gatttgcact ttaaccactt tcggtaaaga aacctttgtt agtgaaagcg aaaaacgcac
    16261 aacgaccact gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg
    16321 cccaacgcat aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga
    16381 tctgggccgc cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga
    16441 tatggcagtg ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc
    16501 tttgctgagt cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc
    16561 gccgcaggaa gatttcacgc tcacttccga ctggcaatcc aatatgaccc gcgagcagta
    16621 cggcgaaaaa tttcgccagg tacaggaata tctgcacagc ggtgattgct atcaggtgaa
    16681 tctcgcccag cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct
    16741 taatcaggcc aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt
    16801 aagcctttcg ccagagcggt ttattctttg tgataatagt gaaatccaga cccgcccgat
    16861 taaaggcacg ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag cagaaaaact
    16921 ggcgaactca gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga
    16981 tatcggtcgt gttgccgtag ccggttcggt aaaagtacca gagctcttcg tggtggaacc
    17041 cttccctgcc gtgcatcatc tggtcagcac tataacggcg cgactaccag aacagttaca
    17101 cgccagcgat ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt
    17161 acgggctatg gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag
    17221 cattggctat ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac
    17281 tgccattaac ggacaaatat actgctctgc gggcggtgga attgtcgccg atagccagga
    17341 agaagcggaa tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa
    17401 gtaagacgtg gaataccgta gcctgacgct tgatgatttt ttatctcgct ttcaactttt
    17461 gcgcccgcaa attaaccggg aaaccctaaa tcatcgtcag gctgccgtgt taatccccat
    17521 catccgtcga ccgcaaccgg gggtgttgct gactcagcgt tcaattcatc tgcgtaaaca
    17581 cgctggacaa gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc
    17641 cgccgcgctg cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat
    17701 cggcgtgctg ccgcccgtcg atagcgtaac tggctatcag gtaaccccag tggtcggcat
    17761 tatcccgccc gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat
    17821 gccgctcgcc caggcattac atctgggtcg ttatcaccct ttagatatct accgccgtgg
    17881 cgattcacat cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc
    17941 aggcataatt cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt
    18001 acatctacaa aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga
    18061 ttagtttaat tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct
    18121 gttattagtt cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct
    18181 attcgacatg tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa
    18241 ggcgggtaaa cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg
    18301 cgttgccgtg gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat
    18361 cgccattatt atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc
    18421 cggttttatt cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga
    18481 agtggatttc ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga
    18541 aaacggtatg caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta
    18601 ttccatcggc ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga
    18661 agtgagcgtg ccgtatccgt tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac
    18721 cggctattcg ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga
    18781 gatcgacgag tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat
    18841 gaacaccgaa ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg
    18901 ccggatgtta gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt
    18961 aaacatgttt gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc
    19021 gccaaccaac ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat
    19081 tgaatcggtc agcccggaca tctatacccg ttactttatg gcagcgggcg cgattggtgc
    19141 attgtataaa atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg
    19201 tgttgcctgt tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca
    19261 ggtttgcgtg gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt
    19321 tgcagggcag gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat
    19381 taacgccgca cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt
    19441 catcgaaacg atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg
    19501 cggtggtctg gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg
    19561 gatgggcgaa tttatacccg ctttctcgtc tgctgtaata ttccccacta cacttccact
    19621 gttgcgtcag gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac
    19681 ggatcattaa aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta
    19741 cgctcgcttc gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg
    19801 tagtacaatt cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg
    19861 caggtagcga agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat
    19921 tacgtaaaca ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca
    19981 cactctattg ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg
    20041 aacttcctgc accgcaacca cttttacgcc tgacgatcga ccgtgccctg attaaaggca
    20101 gtccggtttt gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatggaga
    20161 tgattaacat cgacttactg acggcaatgc tccttgagcc acaactgccg caaatcagta
    20221 gcgccagcct gacggtggac aaacggcatt tgctctatgg taatgggctg gtagattccc
    20281 ttccgcaacc tgaagacaat gaaaactatc aggtttcttc gcaacgcttt ccttttacca
    20341 ttaacgttaa tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac
    20401 cgctggcggt gctgctaagt ttactggtgg gctacatcgc ctggctggcg accgcttacc
    20461 ggatgagctt ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct
    20521 gtcagccttt gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct
    20581 ggaataatcc gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac
    20641 atcatttaat tgtgccactg acccgctatg tgatggcaga aaccattcgt cagcgccatg
    20701 ttttcccgat gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc
    20761 gtggtgtgct gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac
    20821 tgctcctcga aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg
    20881 agctacatcg taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagttcat
    20941 tttcctggct tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag
    21001 ctataggttc tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa
    21061 gactgaatat tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc
    21121 gccgtcatgg cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg
    21181 attttcccaa atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca
    21241 cgcccattat gccgttacgt taatgcttac tcatcttcat cgtgcgccgg ttgctcttta
    21301 acaatgcgaa ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc
    21361 cctacatcaa tcacatcgcc cacacgggga atgtgaccat ttgccgagat cacgaggccc
    21421 gcgaccgtcg cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag
    21481 gcatgcaaat ctgtaccgcc ttttaccagc cagccgtcac catcagtaat gatttccggc
    21541 gtttcgtcag cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc
    21601 agaccttgta ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcacgacgc
    21661 agcacgccca acaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc
    21721 gaagcaatcg ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt
    21781 acaataccga tgatttcatc cagttcaccg cgacataccg ggaacagact gtgcggtgaa
    21841 gagagcagtt gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca
    21901 ccgcgcggcg tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc
    21961 atgtaacgtt cttcttcggc aaatgcacct tccgggatcg gcatcggcat cgggttatcg
    22021 gcatcgtgct gaacattggc ctgacgtttc ccgcccatca aacgcaggat ggcatcggca
    22081 gtacgcgctc gcagcggcaa agtcgactgg tggcgaataa agttgcgacg cgcaatctgg
    22141 ttaaacactt cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg
    22201 tggaaaccga aaccttctgc caccagactc agaccaatca ttaacaggaa gctcagacag
    22261 agcaccacca ccgtggggtg ctggttaacg aatcgcgtca gcggtttgga tgccagcaac
    22321 ataaccgcca tcgcaatcac taccgccgcc atcatcaccg gcagatggtt aaccatccct
    22381 actgcagtaa ttaccgcatc caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg
    22441 accacccaga aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc
    22501 cgttcatgca gttcggttgt tgctttgaac agcaagaata tccccccgaa caacataatc
    22561 aggtcgcgtc cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc
    22621 atccatgaaa tcagcgacag cagccccaga cgcataatca gcgccagtga taaccccagc
    22681 aaacgcgctt tatcgcgttg ttttggcggc agtttgtcag caagaatggc gatgaagacc
    22741 aggttatcga tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatt
    22801 gaggggtcca ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca
    22861 gaaataatgc aggcgtaacg acaaaatgca aacgaaaggt gcggcataga gagccagaaa
    22921 ggtaggcgtt aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta
    22981 ctgccctata ctccatggtt gttaaacggg agttaaacat atcagagacg cctctgattt
    23041 ggcaaagatt taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt
    23101 tctgctaatc gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct
    23161 aaaataaatc gcgaaacgca ggggtttttg gttgtagccc ttatctgaat cgattcgatt
    23221 gtggacgacg attcaaaaat acatctggca cgttgaggtg ttaacgataa taaaggaggt
    23281 agcaagtgac cattgctatt gttataggca cacatggttg ggctgcagag cagttgctta
    23341 aaacggcaga aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag
    23401 gtgaaaatgc cgaaacgctg attgaaaagt acaacgctca gttggcaaaa ctcgacacca
    23461 ctaaaggcgt gctgtttctc gttgatacat ggggaggcag cccgttcaat gctgccagcc
    23521 gcattgtcgt cgacaaagag cattatgaag tcattgcagg cgttaacatt ccaatgctcg
    23581 tggaaacgtt aatggcccgt gatgatgacc caagctttga tgaactggtg gcactggcag
    23641 tagaaacagg ccgtgaaggc gtgaaagctc tgaaagccaa accggttgaa aaagccgcgc
    23701 aagcacccgc tgccgcagca ccaaaagcgg ctccaactcc ggcaaaacca atggggccaa
    23761 acgactacat ggttattggc cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg
    23821 ccacccgctg gaccaaagaa accaatgtct cccgtattat tgttgttagt gatgaagtgg
    23881 ctgcggatac cgttcgtaag acactgctca cccaggttgc acctccgggc gtaacagcac
    23941 acgtagttga tgttgccaaa atgattcgcg tctacaacaa cccgaaatat gctggcgaac
    24001 gcgtaatgct gttatttacc aacccaacag atgtagagcg tctcgttgaa ggcggcgtga
    24061 aaatcacctc tgttaacgtc ggtggtatgg cattccgtca gggtaaaacc caggtgaata
    24121 acgcggtttc ggttgatgaa aaagatatcg aggcgttcaa gaaactgaat gcgcgcggta
    24181 ttgagctgga agtccgtaag gtttccaccg atccgaaact gaaaatgatg gatctgatca
    24241 gcaaaatcga taagtaacgt attgtgttga ttatcactca gttttcacac ttaagtctta
    24301 cgtaaacagg agaagtacaa tggagattac cactcttcaa attgtgctgg tatttatcgt
    24361 agcctgtatc gcaggtatgg gatcaatcct cgatgaattt cagtttcacc gtccgctaat
    24421 cgcgtgtacc ctggtgggta tcgttcttgg ggatatgaaa accggtatta ttatcggtgg
    24481 tacgctggaa atgatcgcgc tgggctggat gaacatcggt gctgcagttg cgcctgacgc
    24541 cgctctggct tctatcattt ctaccattct ggttatcgca ggtcatcaga gcattggtgc
    24601 aggtatcgca ctggcaatcc ctctggccgc tgcgggccag gtactgacca tcatcgttcg
    24661 tactattacc gttgctttcc agcacgctgc ggataaggct gctgataacg gcaacctgac
    24721 agcgatttcc tggatccacg tttcttctct gttcctgcaa gcaatgcgtg tggctattcc
    24781 ggccgtcatc gttgcgctgt ctgttggtac cagcgaagta cagaacatgc tgaatgcgat
    24841 tccggaagtg gtgaccaatg gtctgaatat cgccggtggc atgatcgtgg tggttggtta
    24901 tgcgatggtt atcaacatga tgcgtgctgg ctacctgatg ccgttcttct acctcggctt
    24961 cgtaaccgca gcattcacca actttaacct ggttgctctg ggtgtgattg gtactgttat
    25021 ggcagtgctc tacatccaac ttagcccgaa atacaaccgc gtagccggtg cgcctgctca
    25081 ggcagctggt aacaacgatc tcgataacga actggactaa caggtgagcg aaatggttga
    25141 tacaactcaa actaccaccg agaaaaaact cactcaaagt gatattcgtg gcgtcttcct
    25201 gcgttctaac ctcttccagg gttcatggaa cttcgaacgt atgcaggcac tgggtttctg
    25261 cttctctatg gtaccggcaa ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca
    25321 agctattcgc cgtcacctgg agttctttaa cacccagccg ttcgtggctg cgccgattct
    25381 cggcgtaacc ctggcgctgg aagaacagcg tgctaatggc gcagagatcg acgacggtgc
    25441 tatcaacggt atcaaagtcg gtttgatggg gccgctggct ggtgtaggcg acccgatctt
    25501 ctggggaacc gtacgtccgg tatttgcagc actgggtgcc ggtatcgcga tgagcggcag
    25561 cctgttaggt ccgctgctgt tcttcatcct gtttaacctg gtgcgtctgg caacccgtta
    25621 ctacggcgta gcgtatggtt actccaaagg tatcgatatc gttaaagata tgggtggtgg
    25681 cttcctgcaa aaactgacgg aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt
    25741 ggttaacaag tggacacatg tcaacatccc gctggttgtc tctcgcatta ctgaccagac
    25801 gggcaaagaa cacgttacta ctgtccagac tattctggac cagttaatgc caggcctggt
    25861 accactgctg ctgacctttg cttgtatgtg gctactgcgc aaaaaagtta acccgctgtg
    25921 gatcatcgtt ggcttcttcg tcatcggtat cgctggttac gcttgcggcc tgctgggact
    25981 gtaagactgt tgtacactac cggggccttt tggccccgtt tttttatctg gaggattaat
    26041 gacaatcacg gacctggtac tgattctttt catcgccgca ctcctggcct tcgcgatcta
    26101 cgatcagttc atcatgcccc gccgtaacgg ccccaccctg ctggcaattc ctttgctccg
    26161 gcgtggtcgc atcgatagcg ttatcttcgt cggattgatt gtcattctta tctataacaa
    26221 cgtcacgagt catggtgcgt taataacgac atggttatta agcgcactgg ctctgatggg
    26281 tttttatata ttctggatcc gcgttccgaa gatcatcttt aaacaaaaag gttttttctt
    26341 cgccaatgtc tggattgaat atagccgaat caaagcgatg aacttgtcgg aagatggcgt
    26401 gttggtgatg caattagaac agcgtcggct gttaatccgc gttcgaaata tcgacgatct
    26461 ggaaaaaatt tataagcttc tcgtttcaac tcaataagtt atgaatttag ccaaagctat
    26521 gtttagtgta ttttta
//
LOCUS       DAZGTR010000061        26460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGTR010000061 DAZGTR010000000
VERSION     DAZGTR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.061"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(231..979)
                     /locus_tag="ACHLCY_002739"
                     /pseudo
     CDS             complement(231..979)
                     /locus_tag="ACHLCY_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1058..2011)
                     /gene="lsrR"
                     /locus_tag="ACHLCY_002740"
     CDS             complement(1058..2011)
                     /gene="lsrR"
                     /locus_tag="ACHLCY_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP0369964.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2260..3797
                     /gene="lsrA"
                     /locus_tag="ACHLCY_002741"
                     /pseudo
     CDS             2260..3797
                     /gene="lsrA"
                     /locus_tag="ACHLCY_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            3791..4819
                     /gene="lsrC"
                     /locus_tag="ACHLCY_002742"
     CDS             3791..4819
                     /gene="lsrC"
                     /locus_tag="ACHLCY_002742"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP0369965.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4819..5811
                     /gene="lsrD"
                     /locus_tag="ACHLCY_002743"
     CDS             4819..5811
                     /gene="lsrD"
                     /locus_tag="ACHLCY_002743"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP0369966.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGLTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5823..6845
                     /gene="lsrB"
                     /locus_tag="ACHLCY_002744"
                     /pseudo
     CDS             5823..6845
                     /gene="lsrB"
                     /locus_tag="ACHLCY_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            6872..7747
                     /gene="lsrF"
                     /locus_tag="ACHLCY_002745"
     CDS             6872..7747
                     /gene="lsrF"
                     /locus_tag="ACHLCY_002745"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP0369967.1"
                     /translation="MADLDDIKDGKDFRTDQSQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7771..8061
                     /gene="lsrG"
                     /locus_tag="ACHLCY_002746"
     CDS             7771..8061
                     /gene="lsrG"
                     /locus_tag="ACHLCY_002746"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP0369968.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8118..8876
                     /gene="tam"
                     /locus_tag="ACHLCY_002747"
     CDS             8118..8876
                     /gene="tam"
                     /locus_tag="ACHLCY_002747"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP0369969.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAAKITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(8880..9794)
                     /locus_tag="ACHLCY_002748"
     CDS             complement(8880..9794)
                     /locus_tag="ACHLCY_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP0369970.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDIQFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10001..11452)
                     /gene="uxaB"
                     /locus_tag="ACHLCY_002749"
     CDS             complement(10001..11452)
                     /gene="uxaB"
                     /locus_tag="ACHLCY_002749"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP0369971.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPCDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(11679..13097)
                     /locus_tag="ACHLCY_002750"
     CDS             complement(11679..13097)
                     /locus_tag="ACHLCY_002750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0369972.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(13236..13595)
                     /locus_tag="ACHLCY_002751"
     CDS             complement(13236..13595)
                     /locus_tag="ACHLCY_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP0369973.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(13595..14521)
                     /gene="glsB"
                     /locus_tag="ACHLCY_002752"
     CDS             complement(13595..14521)
                     /gene="glsB"
                     /locus_tag="ACHLCY_002752"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP0369974.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(14585..15973)
                     /gene="sad"
                     /locus_tag="ACHLCY_002753"
     CDS             complement(14585..15973)
                     /gene="sad"
                     /locus_tag="ACHLCY_002753"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP0369975.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAIIVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16074..16955
                     /locus_tag="ACHLCY_002754"
     CDS             16074..16955
                     /locus_tag="ACHLCY_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0369976.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17033..18147
                     /gene="yneK"
                     /locus_tag="ACHLCY_002755"
                     /pseudo
     CDS             17033..18147
                     /gene="yneK"
                     /locus_tag="ACHLCY_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            18297..19487
                     /gene="ydeA"
                     /locus_tag="ACHLCY_002756"
     CDS             18297..19487
                     /gene="ydeA"
                     /locus_tag="ACHLCY_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP0369977.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(19512..20177)
                     /gene="marC"
                     /locus_tag="ACHLCY_002757"
     CDS             complement(19512..20177)
                     /gene="marC"
                     /locus_tag="ACHLCY_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP0369978.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            20389..20823
                     /gene="marR"
                     /locus_tag="ACHLCY_002758"
     CDS             20389..20823
                     /gene="marR"
                     /locus_tag="ACHLCY_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP0369979.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            20843..21226
                     /gene="marA"
                     /locus_tag="ACHLCY_002759"
     CDS             20843..21226
                     /gene="marA"
                     /locus_tag="ACHLCY_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP0369980.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21258..21476
                     /gene="marB"
                     /locus_tag="ACHLCY_002760"
     CDS             21258..21476
                     /gene="marB"
                     /locus_tag="ACHLCY_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP0369981.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(21533..22972)
                     /locus_tag="ACHLCY_002761"
     CDS             complement(21533..22972)
                     /locus_tag="ACHLCY_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0369982.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIVWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(22997..24670)
                     /locus_tag="ACHLCY_002762"
     CDS             complement(22997..24670)
                     /locus_tag="ACHLCY_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP0369983.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(24726..25037)
                     /locus_tag="ACHLCY_002763"
     CDS             complement(24726..25037)
                     /locus_tag="ACHLCY_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP0369984.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25065..26387)
                     /locus_tag="ACHLCY_002764"
     CDS             complement(25065..26387)
                     /locus_tag="ACHLCY_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP0369985.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT         6871 a         6575 c         6234 g         6780 t
ORIGIN      
        1 attaaacccc ttttgcatcc attaatgtct ttaggaatat tcgctataaa ataagggtta
       61 tgcttaatgc tttaagaaaa atagcaattt tcccttgaat atagtactgg tgatggaacg
      121 atgaatctgc aagctggcat ttaacaagcc agctctaaaa gaagggaaat aagaaaaact
      181 atactcaaaa actaacagcc acggtcatca tgatgtggct gtcaatgaaa ctataaccca
      241 ggcgctttcc ataacgacgt cgtcagtcca tgatcaacca gccccagctg atcctgataa
      301 actgcctgcc acttatcgcg tgaatcctga taaagttcat gcttttccgg gtctggtgtg
      361 tgcgtccgtt cccagcgaac caggcgttct ccggtttctg ccattgatga aaaaattccg
      421 gcaccgacgc cagttcgccg ctgagcatat aggccagcca gtcgctgatc atggtgatgg
      481 ttgatgcctg acggtaaata tcggaacgat ggtgcgccag ccaaagtaat ctggggatgg
      541 cacttaaagc cagtgtttgt ccggtcgcgc gataaacttc gttttcaaag gtattgttgt
      601 gcagttcttt aagttcgcta acttcgcgcg ctgctctggc atccacattg gcgcaggccc
      661 agatcggtgt tccttcattg ttatataaaa caatgccttc acgcatagaa catgccgaaa
      721 cggcagcgat atactccggg tctatgccgg cgttgtgcag cgcctggcgc atacactcac
      781 acgccagttg ccagtttttg ttgagatcaa attccataga accaggaacg tccggtactg
      841 ccagatgccg ccactccgcc tgtcccactg ctatttgatt gcctttcagg tcgaaaatca
      901 cagcccgaat acttccggtg cctgcatcca gcgccaacag gtaatacttt gattctgaag
      961 gggtaaagag tcgagccatt agattatcct cggcttatgc gttctgtctg ttcctctata
     1021 cgttctccat cattcccggt aataaggtca tgcaaattta actacgtaaa atcgccgctg
     1081 ctgtatcctg atcggtaacc agtgcgttga tataaccgcc tttcattgca gcggcaattg
     1141 cttcggcttt attttctccc cctgccacgc caacccggac gggtatggtc ttcagcgcgc
     1201 ttaaaggtaa gccaatcagt tcgttatgta ttttaatatc cgtgacaacg tcaccttttg
     1261 catcaaaaaa gtagcctaaa atgtcgccaa ccgccccttt tcgaccaatc attaactgtt
     1321 cgccctggct gatataaccg gagcgaatga tcgtcgcgtc gtcctgttga ctgacggccc
     1381 caatgccgac aatcgccaca tccgctgctt gcgcggctaa cagaacatct ttgacgcaat
     1441 tttcattttt tagcgtacgg gcaatgtcag cggaggatgc ccgcaacgga gccggaataa
     1501 tattcacact gcacgccgcg ttaagctgcc cgatacccgt catataagaa ccgacgccac
     1561 cggagagcgt gaccaggcga atttgctgtg acgaaataaa accacttaag cgttgcagcg
     1621 tattcatggt tgcctcgcca aaaccaatcg ccagcatctg ttgtggttga agtaaactca
     1681 tcaacatatg cgccgcgcct atccctagtc gcccgccgac atcagcatcc gcaagcccag
     1741 ggatcacccg gacatgttgc agcgaaaact gacgacgtaa ttgagtttca tattccagac
     1801 agccttcaaa gcgagaatta atctgtacgc gaataatgcc ggactgatgc cctttctcca
     1861 gcaagcgcga cactttcaaa cgggtcaggc cgagacgatc gctgatctcg ctctgggtca
     1921 gcccgtcgtg atagtaaaac cacgcgatcc gcgcgacctg ttcttcttca cacattccct
     1981 gttctgaaat tgccgaatcg ttgattgtca taattcattc ttcactttga acatatttaa
     2041 atctttaatg caattgttca gttcttgcgc atttatatct gtgatggcag ccacagtttg
     2101 actctacgag catgaacaaa cgcaaccgtg aaaatcaaaa tagcataaat tgtgatctat
     2161 tcgtcggaaa tatgtgcaat gtccacctaa ggttatgaac aaattaaaag cagaaataca
     2221 tttgttcaaa actcacctgc aaaactgaac gggggaaata tgcaaacgag tgatacccgc
     2281 gcgttaccgc tactttgcgc ccgctcggtt tataaacagt attcaggggt caatgtcctg
     2341 aaaggcatcg attttacgtt gcatcagggg gaggtccacg ccctgctcgg cggcaatggt
     2401 gccggtaaat cgacgttaat gaagattatt gccggtatta cccctgctga tagcggtacg
     2461 ctggagattg ggggcaacaa ctacgccaga ttaacgccag ttcatgctca tcagctgggc
     2521 atttatctcg ttccccagga accgctgctt ttcccaagcc tgtcgataaa agaaaacatc
     2581 ctgtttggga tggcaaaaaa acagctctcc atgcaaaaaa tgaagaactt gctggcggcg
     2641 ctgggctgcc agtttgatct gcatagtctg gcaggatcgc tggatgtcgc cgatcgccaa
     2701 atggtggaaa tcctccgcgg gctaatgcgc gactcgcgga ttctgatcct cgatgaacct
     2761 accgcctcgc ttacccctgc ggaaaccgaa cgcttgttta ctcgcttgca agagctgctt
     2821 gctactggcg tgggtattgt ttttatctcg cataagctgc cggaaattcg ccagattgcc
     2881 gatcgaatta gcgtgatgcg cgacggaacc atcgccttaa gcggcaaaac cagcgaactg
     2941 tctaccgacg acattattca ggccatcacg ccagtggtac gggaaaaatc gctctctgcc
     3001 agccaaaaat tatggctgga gttacctggt aaccgcccac aacatgctgc cggaacgccg
     3061 gtgctgacac tggaaaatct gaccggcgaa ggtttcagga atgtcagcat gacgctcaat
     3121 gccggagaaa ttctgggcct ggctgggctg gtgggggccg gacgcacaga actggccgag
     3181 agacgttcta tggtctgcgt actttgcgta gcggacgcat tatgctgaat ggtaaagaga
     3241 tcaataaatt atccactgga gaacgtttac tgcgcggtct ggtttatctg ccggaagatc
     3301 gccagtcatc cggactgaat ctcgatgctt cgctggcctg gaacgtctgc gcccttactc
     3361 ataaccttcg tggattctgg gcgaaaaccg cgaaagataa tgccaccctg gaacgttatc
     3421 gtcgggcgct gaatattaaa tttaaccaac cggaacaagc tgcacggaca ttatccggtg
     3481 gcaaccagca aaaaatcctc attgccaaat gcctggaagc ttcgccgcaa gcattgattg
     3541 tcgatgagcc gacgcgcggc gtggatgtct cgtcccgtaa tgatatctac cagctgttgc
     3601 gcagcatctc cgcacagaat gtggctgtgc tgcttatctc ctctgatctg gaagagatcg
     3661 aactgatgac agatcgtgtg tatgtgatgc atcagggcga aattacccac tctgcactga
     3721 ccgggcgcga tattaatgtc gagactatta tgcgcgttgc cttcggcgat agtcagcgtc
     3781 aggaggcatc atgctgaagt ttattcagaa caaccgtgaa atcacggcat tgctggcggt
     3841 ggtgctgctg tttgtattac ccggttttct cgaccgtcag tatttaagtg tgcaaacgct
     3901 gaccatggtt tatagcagcg cgcaaatcct gatcctgctg gcaatgggcg cgacactggt
     3961 aatgcttacg cgcaatattg atgtttcagt gggttcgatt accggaatgt gcgcggtgct
     4021 gttggggatg ttactgaacg caggatattc actacctgtt gcctgtgtcg cgactttact
     4081 gcttggtttg ctcgcgggat ttttcaacgg tgtcctggtc gcgtggctaa agatccctgc
     4141 cattgttgcc acccttggca cgttagggtt gtacagaggc atcatgttgc tgtggactgg
     4201 cggtaaatgg attgaagggt tacccgccga actgaaacag ctctccgcac cgctgctgtt
     4261 tggcgtttcc gcgattggtt ggttgacgat aattctggtg gcatttatgg cctggctgct
     4321 ggcaaagacg gcgtttggac gcagttttta tgccacgggc gataatttac agggcgctcg
     4381 tcaactgggt gttcgtacgg aagccattcg cattgtggca ttttcgttga acggctgcat
     4441 ggcggcactg gcgggaattg tgtttgcttc gcagattggt tttatcccca accagaccgg
     4501 taccgggctg gagatgaaag caattgcagc ctgcgtgctg ggcggcatta gtttgctcgg
     4561 cggttccggt gcgatcatcg gtgcggtact cggcgcatgg ttcctgacgc agatcgatag
     4621 cgtactggtg ctgttgcgca ttccggcatg gtggaacgat tttatcgcgg gcctcgtgct
     4681 gctggcggtg ctggtgtttg atggacgcct gcgttgtgcg ctggaacgta atctacggcg
     4741 gcaaaaatat gcccgcttta tgacgccacc gccatccgtt aaacccgctt cgtcaggtaa
     4801 aaaacgggag gccgcataat gcgtattcgc tacggttggg aactggctct tgccgcactg
     4861 ctcgttattg agattgtcgc atttggtgca attaacccgc gaatgttaga tctcaatatg
     4921 ttgctgttca gcaccagtga ctttatctgc attggcattg tcgccctacc gctgacgatg
     4981 gtgattgtca gtggcgggat cgatatttcg tttggtttga ccatcggcct ctgcgccatt
     5041 gcattgggcg tactgtttca aagtggtgtg ccgatgccgc tggcgatact cctgacctta
     5101 ctgctcggcg cattgtgcgg gctgatcaac gccggattaa ttatctatac caaagttaac
     5161 ccgctggtga ttacgcttgg cacgctgtat ctgtttgccg gaagcgctct gctgctttcc
     5221 ggtatggccg gagcgacggg gtacgaaggt attggtggat tcccgatggc gtttacagat
     5281 ttcgctaacc tggatgtgct gggactcccc gttccgctga ttatcttcct gatatgtctc
     5341 ctcgttttct ggctctggct gcataaaacc catgccggac gtaatgtgtt tttgattggg
     5401 caaagcccgc gcgtggcgct ttatagcgcg attccagtta accgcacctt atgtgcgctc
     5461 tatgccatga cggggctggc gtctgcggtc gccgctgtgc tgctggtatc gtattttggt
     5521 tcagcacgtt ccgatctcgg tgcgtcgttt ctgatgcccg ccatcaccgc cgtggtgctt
     5581 ggcggggcca atatttatgg tggttccggt tccattatcg gcaccgccat tgcggtttta
     5641 ttagtgggat atttgcagca aggtttgcaa atggcaggag tgccaaatca ggtgtccagc
     5701 gccctttccg gtgcgctact tatcgtcgtt gtcgtaggtc gttccgttag cctgcatcgc
     5761 cagcaaatta aagagtggct ggcgcgtcgg gccaataacc cattgccata aaggatatct
     5821 tcatgacact tcatcgcttt aagaaaatcg ccttacttag cgctcttggc attgccgcaa
     5881 tctctatgaa tgtgtaggcc gcagagcgta ttgcatttat tcccaaactg gttggcgtgg
     5941 gattttttac cagcggtggc aacggcgcac aacaagcggg taaagagctg ggcgttgatg
     6001 tgacctacga cgggccgaca gaacccagtg tttctggtca ggtacagttg attaataact
     6061 tcgtcaatca aggttataac gccattatcg tttctgcggt ttcgcctgat ggcttgtgtc
     6121 cggcactgaa acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta
     6181 aaccggagtg ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt
     6241 tggtggatat ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact
     6301 caagccccac cgttatggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca
     6361 aagagcatcc tggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat
     6421 cattacaaac cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc
     6481 ccgacgccaa cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag
     6541 ttgcgattgt cggattcagt acgccaaacg tgatgcgtcc atatgtggaa cgcggcacgg
     6601 tgaaagaatt tggcctgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg
     6661 atgcattatt gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag
     6721 gtcaggtgga agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg
     6781 gcatcgtact gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt
     6841 tctgatgtgc attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg
     6901 taaagatttt cgtaccgatc aatcgcaaca aaatatccct tttatcctga aaggttgcgg
     6961 tgcgctggat tggggaatgc agtcacgctt atcgcggata tttaatccga aaacgggtaa
     7021 aaccgtgatg ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg
     7081 cattgatata aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg
     7141 cattttgcgc agcgtagttc ccccagcgac caataagccg gtggtactgc gggcgtcagg
     7201 tgcgaactct attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc
     7261 cgtgcgcctg aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca
     7321 tcagtcgatc aaaaatatta ttcagctggt tgatgccgga atgaaagtgg gaatgccgac
     7381 catggccgtg actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc
     7441 gactcgaatc gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg
     7501 ttttgaacgg attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt
     7561 accggagcgc gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt
     7621 ggatatgggg cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca
     7681 ggcggtggtt caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa
     7741 acagtaactg cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt
     7801 catgaagaca agattgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta
     7861 caggaagaag gcaatttgcg cttcgatgtc ttacaggacc cagaagtgaa ttcgcgcttt
     7921 tatatctacg aagcctataa agatgaagac gcagtggcat tccataaaac cacgccccac
     7981 tacaaaacct gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg
     8041 ttcaatgggt tgatgccgtg aggcgaattc atcagtttta tctacaattg gggtaacgcg
     8101 ctgacgggag taaataaatg tctgactgga acccctcttt atatctgcac tttgccgctg
     8161 aacgatcgcg tccggcggtg gagctgcttg ccagagtacc gctggagaat atcgaatacg
     8221 ttgccgatct tggctgtggc ccaggtaaca gcaccgccct tctaaaccaa cgttggcctg
     8281 ctgccaagat aacaggcatc gactcgtctc cggcgatgat tgctgaagcg cgcagtgctt
     8341 tgccagactg ccagttcgtg gaagcagaca tccgcaactg gcaaccggaa caggcactcg
     8401 atctgatttt tgctaatgcc tcactgcaat ggctgcccga ccactacgaa ttgtttcctc
     8461 atctggtttc tttacttaat ccgcagggcg tgctggcagt acagatgcca gataactggc
     8521 tggagccgac ccatgtgctc atgcgcgaag ttgcctggga acaaaactac ccggatcgcg
     8581 ggcgtgagcc gttggctggc gttcatgctt actacgatat tttgagcgaa gccggatgtg
     8641 aggtcgatat ctggcgaacc acttactatc accagatgcc gtcgcaccag gcgattatcg
     8701 attgggtgac cgccactgga ttacgtccgt ggttacagga tctgaccgag agcgaacagc
     8761 agctttttct tacgcgctac catcagatgc tggaagagca gtatccactg caagagaacg
     8821 gacagatact gctggcattt ccgcgtctgt ttattgttgc ccgccgtacg gagtaattat
     8881 cacgtcagct ggtaatgacg atcgggaaga atttttgctg gaatttctgc ttcatcgttc
     8941 atctgtaaca ggtcaatttc aatagcgttg cagatggcat ccagcggtaa atcattgttt
     9001 tcagtaccga acggatcttc cagttcttcc gccagacaat ccagcgaaat aaaagtgtag
     9061 gaaatcagca cagagataaa aggcgtcatg taatgcaggt ccacgaccag cgcgaacggc
     9121 agcatgatac aaaacagata aacggtacga tgcaaaatca gggtgtaggc aaagggaatt
     9181 ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg aaatatcatt aagccgatcg
     9241 ttgaggctaa taaactggat atctgaaagc tgcccattgc ggcgctgaac cgccaaccac
     9301 tcccccatta ttaacaagat acggttagct ggagagttcg aagccagtac aggctgaaga
     9361 tcttcagtct tgagataatg agccagcact tccgcctgtg gctgtttgcg taatgtcatg
     9421 cgtaaacagt gggcgaaggc gatttgcaac cgggcaaact cccttacact tgccgaatcc
     9481 ggcaatgtcg tttttacctc gcgcagtaac gaccgtgagg caatcatcaa ctgcccccaa
     9541 agttttcgcg cttcaacgta acgggcgtac ccggcattat tacgaaaacc aagaaaaatg
     9601 gcgatggcga caccgagaat gctgaacggt gcgagggtga atttgatgcc cagatgcgtg
     9661 taccagggca gcatgaaaat aacagcgata gaaaagagaa aattgagtag taagcgcgat
     9721 gatatcttgg ataatactga gccgtgccag acaaaaatac ggcgcagcca gtgttgttgt
     9781 ggacgaacaa tcatggttat cttcaggcgt gaaaaagtcg ccctattaaa cgtgattacg
     9841 atcacattct caagacgcta cttacaaatt acctacaagc ttacaacatt aacaggcgct
     9901 gcatgtggca tctgttaatg ataaaggcac ataggatgtt gtaactaata tagtgaaata
     9961 agaaacccgg tcgaaaccgg gttcagaagt aacggtgtta ttagcacaac ggacgtacag
    10021 cttcgcgcat ccctttttca agaatcgcat ccaggtcatt agcaacctgc tcgaccagac
    10081 caggcacttg cgtcaggtcc tgctcccagt ggtctttttc tgccaacaca atcgctacca
    10141 gttcctgggt accgataaca cggtcacgat gctggctcca gagttgctga taacgttcca
    10201 gccagtgtgc atcgtcctgt accggatatg tttctccgtt acgctcacca cgatagaagg
    10261 caatcaatgc cgctaatgcg aaagtaaggc gcgccggaag tgtgcctttt gccttctgcc
    10321 ctgccagcag ctgcggcagg atgcgagtgc ggaacttggt cataccgttg agcgcgatag
    10381 acagcagctg atgcttaatg tacgggttac ggaaacgccc ggtgactgca ctggcgaaag
    10441 attccagttc atcacgaggc aaatccagta ccggaataat ttcttcgtag atagcttttt
    10501 caacgaatgc gcaaatttca gcatcgttca tcgcttcacc tacggtatcc agccctgcct
    10561 ggaacgccac cggcaccagc gcggtgtgcg caccgttgag gattgccact ttgcgttctt
    10621 tgtacggttt aatatcgtca acaatcagca cgttgagcgg atatttgtcc agacgcagtt
    10681 cagtcgctaa ggatttcggt ccctgaatca caaacaggta aaagtgttca gcggtgtcga
    10741 gaaaaccatc gtgataaccc agctcttctt ccagtttagc cacttcatcg cacggataac
    10801 cggtaacgat acggtccacc agcgtagaac agaagctgtt agcctgatcc agccattgaa
    10861 taaatgcttc tggcagtgcc cactcttgcg catagcgcag caccagttca cgcaacgcgt
    10921 cgccattgta gtcgatcaac tcacacggga tgatgatcca acctttatcc agcgcaccgt
    10981 tgaaatggct aaaacgttcg aacagcagac gggtcagttt tgccggataa cttaccgctg
    11041 gcgcgtcatc gaatttatcg cccgcatggt agctgatacc cgcttcggtg gtattagaga
    11101 aaacaaagcg catttccggg ttgtgagcca gtttcaggaa ttcatcgtat tcactgtaga
    11161 cgctgatttc acgattaacc gagcgaatca gacgcgcgtc gctgaccgct tcccccttct
    11221 cattcaggcc acggataatg gtggtataca gaccatcctg cgtgctcagt gacggtggga
    11281 atgaagtttc aatcggacga acaacgacca caccagaatt cagatcggtg tgctcattca
    11341 ggagatcgat ttgccagtca acaaaggcgc gcaggaagtt accttcacca aactgaatga
    11401 tacgttctgg atactgtgca ccgggaaaat cgcgacgatt tagtgttttc acaatgggtt
    11461 cccttctgat tagtcataca acctgcttga attggtgcga caggttagca aactttaata
    11521 cgccgaaccc ctgttttgat caactcctga tgattaatga gcagttttat gagaaaagtg
    11581 tggcgcggat catggtttaa tcgaggaaat acgccttttc ctggatcata aagtggtaga
    11641 acacattgca ttcaaatcgc gcggaatgaa taaagatgtc agacaacttc ctcaccgtaa
    11701 cgcatagtgc tggtacggtt gcgcccatct ttcttcgagc gatacagaca cgtatcggct
    11761 tcgaccatca atttattaaa atcatcggtc agcgttcggt acgatgcgcg accactaccg
    11821 acgccaatac ttaccgtgag ataaagcgtt ttttgttgcc aggtgaatgg ttgcagttca
    11881 acgcctttac ggattttttc cgccattagc agaccatcga caggattcac cgacggcact
    11941 gcaacagcaa attcttcgcc gcccatgcgc gccaccagcc ccttatcacc gacaatcttc
    12001 tgaatatgcc gggcaaacac gcttaacact ttatcgccac attcatgtcc atagttatcg
    12061 ttaatgcttt tgaaatagtc gatatcaagc agcatgaccg tcagatgttg tgtctgtttc
    12121 agcgatggac ttttcagcgc ctcataaaga ccggaccgtg agtaaacctg agtcagaaaa
    12181 tcaaagtcgg ctcgcagcgc aacttgcttc attagcgaat tgatcgctgc cacgctaaaa
    12241 gaaaccataa ttgggcatat cgccatcgtg gcaataccga gacgtgcgga gaacatttcc
    12301 ggaatggaga acggcgaacc gaccgagata tcaatcaccg aatttgccac cagtacgatt
    12361 tccaccgcac cggtgacaaa ggtcaacaga catgttacct gcggcgtata gcgcactgca
    12421 caccagatta atgcaggcag cgggaacgcc agactccccg ctccgccaat gactaccgag
    12481 gcaataaccg acacaataag cgcgatggcg ggcatcatct gttctgcttt aaagcgcggc
    12541 agcactccag gaatagccaa cgtcagcata caaggcacga tcaacacgcc cgttgagaat
    12601 tgctcactga accagtccgc aagcaaaggc cagaaatcca gactatcaat actgaccgaa
    12661 ccaatcgccc cgacaatagc gcataataat gcggcaatca gacagtaatt aaatagccgt
    12721 aaggcactta ctggctcata cttatttttc cccagacgct tatcccgcac gaccagtaag
    12781 gcgacggtaa caataaacat catattggag aaattgataa ccagtgaaac cagtccccat
    12841 tcggtggtta tggcatcata aaccagcatc gccacataac tgatcgcata gtaatgcagt
    12901 cgattaagcc agacatagcg ggcaaacacc cccgccatta cgccattcag cggccagaaa
    12961 agagaaagtt cgtgaaccag ccgtaactct gcaccaataa aatagaacag cgtggttagc
    13021 gcaaaaatag cgatgctatt acgtagcaga tgaccttcct ggaacaaacg gaaagttgaa
    13081 atggggtgta catgcattaa ttatctgtcc atacaattgc gggtggcaat tatctcaacg
    13141 taataagtaa attctcataa aggatagcat gttcgctaac agcggcggca aataaaggta
    13201 ccctgggaat gctattttat cgttttccgc aattctcatg gctgattcat ctgtacaacc
    13261 agccagtgat agattaccgc cacgatataa cgttgttgtt cttcgcttaa cgaaacaccc
    13321 tgcggaatgt tatgccactt agccagacaa ccgcgacagc aggtcgcggt ggcgtgctga
    13381 gcgataaaca ccggatgccc gcgcatgggg gtttgcttac cgtcattagc cggtaatgcc
    13441 ggagcaagac gtttagcgac aaaatccgcc gcatgttgtt cgatgactgg cgcgcctttc
    13501 tccaggcaat actgacgctc tttcatgccc agacgaaagc gagagcgaaa ttttgaacgg
    13561 gataaacgcg cgaagagtgg atcgagagac tgcattaata aaccgaacgc cctaactgtt
    13621 tcgtcaattg ttcaaggacg gcaatacccg caagcgagtt acctgcatca tccagttccg
    13681 gactccagac agcgatggcc atttcatgcg gaacaatcgc cacaataccg ccaccaacgc
    13741 cagatttcgc cggtagcccc acccgccagg caaactcccc cgcgttctgg tacataccac
    13801 tggtcgccat cagcgcgtta atttgccgcg cctgcattgg cgtcaccaca ggttcatcaa
    13861 tatgaatagc tttcccctga ttagccagaa agacaaacgt cctggccagc tctacacagc
    13921 tcattttcag agcgcagtaa tgaaagtagt tttgcagaac ggttgtcaca tcatgatgga
    13981 aattgccaaa cgacttcatc agccaggcga tagccgcatt tcgcgcggaa tgttcaaatt
    14041 cggaacgcgc taccaccgta tcgtatgaaa tatcagacac accgcttaag ccgcgcacga
    14101 cttccagcat acgttgccgt ggtgcgctta atcgcccttg caacatatcg caaaccacca
    14161 gcgcaccagc attaataaac ggattacgcg gaataccctg ctccatttcc agttgcacta
    14221 aagaattgaa cggtgatcca gacggatctt tgccgacgcg ttgccagatt tcctcttcgg
    14281 agtaatgacg catggcgaca acgagactca gcactttgga aatagactga atggaaaaac
    14341 gttcttgcgc gtctccggcc tgaaaaagct gtccgtcaac ggtacagata gcaatgccca
    14401 atcgggaacc gtctactgta gccagcgccg gaatataatc cgcgacttta ccctgaccaa
    14461 tgagcggccg cacttgccgc aagatgtttt ctaaaattgc attatccatg gcgactgcca
    14521 ctttctactc ctggaccgca ggtctgaaaa gacctgcgag tatatcagag ctgaatatgt
    14581 cgcgtcagat ccggtctttc cacaccgtct ggatattaca gaattcgtgt aagccgaaat
    14641 gggaaagctc acgaccaaag ccactctttt tcacgccacc aaaggccact cgcgcgtcgc
    14701 tggcacaata accattgata aacaccccac cgcattccag acgtgccgcc atctgtctgg
    14761 cctgtgtttc gtcggtggta aaaatggtcg ctgaaaggcc gaactcacta tcattagcca
    14821 gttccagcgc atgttctgca tctttcgcaa cgatgattgc cgcaacaggg ccaaacattt
    14881 cttcccgaaa cgcggtcatt tctggggtaa cattcgccag aaccgttggc ggatagtagt
    14941 tacctgcccc agccatcttt tccccgccca gtaacaaacg cgcaccctgc gccagggttt
    15001 tctccacctg atgatgcagc tcatcacgta aatcaaaacg agccattggt ccgagagcat
    15061 tctcttcatc acggggatcg cccattttca aggctgccgc agctgccaca aaacgttcgg
    15121 taaatgcaga agcaattccc tcttcgataa taaagcgttt tgccgctgca caaacctgtc
    15181 cggtattctg ataacgtccg gctaccgccg ctttaaccgc cagttccaga tcggcatcgt
    15241 taagcacaat aaatggatcc gaaccgccca gttccagtac gcattttttc agtgccgctc
    15301 cagcctgtgc gccaatagcc gctcccgcac gaacacttcc ggtcaccgtg acagcagcaa
    15361 tgcgcgaatc tttaatcatt tgactgacac cgtcgttgtc ggcattcagc cagccatata
    15421 cgccttgcgg gattcccgca tctttaaaca cctgggcaat gagctgagca cagcccatca
    15481 cattcggcgc atgtttaagt aagtagccgt tacctgcaag aatgatggga accgcgccac
    15541 gcatcacctg ccataacgga aagttccacg gcataatcgc cagaatcgtc cccaacggtc
    15601 gatactcaat aactgcctgc tgattttcca ccagcgtagg ttccgccttc agcattgccg
    15661 gaccatgttc tgcataccag tcacacaaat tcgccgattt cgccacttca gcgcgcgcct
    15721 gattgattgg tttgcccatt tcacgggtga tcatttgcgc catttcttcg ctacgggcgc
    15781 gcagagcctt accgatacca cgcagttttt cagcacgata atctatattt gtctcgcgcc
    15841 agtcgcgaaa gcctgctgcc gccagctgaa gtgcgttttc gatatcgtta gcgccagccc
    15901 acggcagcac agaaagttgt tcacccgtgg caggatttat tgaaattgca tgagttgccg
    15961 gagtaatggt catcggggta tctcctttat gagtcatggt atgaagatac gcagatttac
    16021 tcttgcttta aaatgaataa tattaagcca cttattcacg aatcgagaat gttatggatc
    16081 tgacccaact ggagatgttc aacgcggttg ccgaagctgg cagcattacc caggctgcag
    16141 caaaagtgca tcgcgtaccg tcgaatctca ctacccgtct acgccagctg gaaacagaac
    16201 tgggggtaga tctgtttatt cgcgagaatc agcgtttacg tctctctcct gccgggcata
    16261 actttttacg ctatagccaa caaattctcg cgttagtgga tgaagcgcgg agcgttgtcg
    16321 ctggcgatga gccgcaaggt ttattttctc ttggttcgct ggaaagcacc gcagcagtgc
    16381 gcattccggc cacgctggcg gaattcaacc atcgttatcc caaaattcag ttttcacttt
    16441 ccaccggccc ttccggcact atgctggaag gagtgctgga gggaaaactg aatgcggcat
    16501 ttattgatgg gcccattaac catactgcca ttgacgggat accggtatac cgcgaggagc
    16561 tgatgatcgt cacgccacaa ggtcatgcgc cagtaatccg cgccagtcag gttaacggca
    16621 gtaacattta tgccttccgc gccaattgtt cgtatcgtcg ccacttcgag agctggtttc
    16681 atgctgacgg tgccgctccg ggaactattc acgagatgga gtcgtatcac gggatgttgg
    16741 cctgtgtggt cgcaggagca ggcattgcgc ttattccacg ctctatgctg gaaagtatgc
    16801 cggggcatca ccaggttgaa gcgtggccgt tagctgagca atggcgttgg ttaacaacct
    16861 ggctggtctg gcgtcgcggt gcgaaaacgc gtccgcttga ggcatttatt caactactgg
    16921 atgtgcctga ctcggcaaaa cagggttacc aatgagctat ttttgatagt tcttgcagct
    16981 caaatgccct atatagtgat gttccgatga cttatgacta tatggggcaa atatggttac
    17041 gccagtaagc atcagtaatt acatatctct tcctgatgat tttcccgtac gtaatattgc
    17101 acctcaggta aaagaagttt taaaggactt tattgatgcg ctaagtacaa taatatgtga
    17161 tgaagagtgg cgcacgtctt taaacatcaa ctcagccaca aaaaagatat ttaataacct
    17221 tgataactta tcatatattc agaggacttc ttttcggggt aacgacacgc tatacaatga
    17281 aaaggttcag tttaaactca cttatcccgt taagaatgga agacacaaag aaaatattga
    17341 atttcaggta gtaataaatt taagccctat ttatttagat aattttcgcc atgatggaga
    17401 aattaatatt tttgcgctcc caacccaaag cctgtcacta tggggcgcgt atttcagacc
    17461 ggcgtcgagc gtgtgctatt tctgtttctg aatgatttta ttgaacaatt tccaatgatc
    17521 aaccttggcg ttcccatcaa aagagcgcat acaccacata ttgaacccct gcctccggat
    17581 cgccataccg ctgcagatta cttacgccag tttgatttgc ttgtcctgaa ttttatctct
    17641 cgcggtaatt ttgtcatact cccccgatta tggagtaact ctgaggttca cagatggttc
    17701 gtcaataaag atcctaactt gatcaccgcc attctcgaca taacagacgg cgaattaaaa
    17761 gaggatttat tgcaaagcct gatggattca ttaggttcta acaaacatgt actacccgaa
    17821 gtctgcatct gctttttatc ccttttagca gaacaagaat ctcctcattt tcaggacttt
    17881 tttctatttt tcgccaatat gttattgcac tatcaccaat ttatgaatcc caatgaaagt
    17941 gatctgaatg acgtgttgat gccagcatca ttaagtgatg ataaaattat caaacatatg
    18001 gcgcgcagga ccctcaaact gtttgtaaaa aatgaaacgc cgccaaaagt tactcacgaa
    18061 gacctggtga aaaacaggcc tcgctcccct gtcagaccac ctatacccgc aaccgccaaa
    18121 acgccagacc tccctgaacg tcattaaacc gtgatgttac cgactctctg acgcggaaca
    18181 gaatcagcgt cagagaaacg gaaaacgcga tccagatcac aaatgcattg tattcacatc
    18241 attaaccgtt ttaagatcat ttcatcactt tttcgcaact cacccgataa tctgttatga
    18301 caacaaacac tgtttcccgc aaagtggcgt ggctacgggt cgttacgctg gcagtcgccg
    18361 ccttcatctt caacaccacc gaatttgtcc ctgttggcct gctctctgac attgcgcaaa
    18421 gttttcacat gcaaaccgct caggtcggca ttatgttgac catttatgca tgggtagtgg
    18481 cgctaatgtc attgcctttt atgttaatga ccagccaggt tgaacggcgc aaattactga
    18541 tctgcctgtt tgtggtgttt attgccagcc acgtactgtc gtttttatcg tggagcttta
    18601 ccgttctggt gatcagtcgc attggtgtgg cttttgcaca tgcgattttc tggtcgatta
    18661 cggcgtctct ggcgatccgt atggctccgg ccgggaagcg agcacaggca ttgagtttaa
    18721 ttgccaccgg tacagcactg gcgatggtct taggtttacc tctcgggcgc attgtgggcc
    18781 agtatttcgg ttggcgaatg accttcttcg cgattggtat tggggcgctt atcacccttt
    18841 tgtgcctgat taagttactt cccttactgc ccagtgagca ttccggttca ctgaaaagcc
    18901 tcccgctatt gttccgccgc ccggcattga tgagcattta tttgttaact gtggtggttg
    18961 tcaccgccca ttacacggca tacagctata tcgagccttt tgtacaaaac attgcgggat
    19021 tcagcgccaa ctttgccacg gcattactgt tattactcgg tggtgcgggc attattggca
    19081 gcgtgatttt cggtaaactg ggtaatcagt atgcgtctgc gttggtgagt acggcgattg
    19141 cgctgttgct ggtgtgcctg gcattgctgt tacctgcggc gaacagtgaa atacacctcg
    19201 gggtgctgag tattttctgg gggatcgcga tgatgatcat cgggcttggt atgcaggtta
    19261 aagtgctggc gctggcacca gatgctaccg acgtcgcgat ggcgctattc tccgggatat
    19321 ttaatattgg aatcggggcg ggtgcgttgg taggtaatca ggtgagtttg cactggtcaa
    19381 tgtcgatgat tggttatgtg ggcgcggtgc ctgcttttgc cgcgttaatt tggtcaatca
    19441 ttatatttcg ccgctggcca gtgacactcg aagaacagac gcaatagttg aaaggcccat
    19501 tcgggccttt tttaatggta cgttttaatg atttccagga tgccgttaat aataaactgc
    19561 acacccatac ataccagcag gaatcccatc agacgggaga tcgcttcaat gccacccttg
    19621 cccaccagcc gcataattgc gccggagctg cgtaggcttc cccacaaaat aaccgccacc
    19681 aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat cagcgaaggt tgaactctga
    19741 cgcactgtgg acgccgagct aataatcatc gctatggttc ccggaccagc ggtacttggc
    19801 attgctagcg gcacaaaggc aatattggca ctgggttcat cttccagctc ttccgacttg
    19861 cttttcgcct ccggtgaatc aatcgctttc tgttgcggaa agagcatccg aaaaccgata
    19921 aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa tcgaaatgcc aaatgtatcc
    19981 atcaccagtt gcccggcgta atacgccacc atcatgatgg caaatacgta caccgaggcc
    20041 atcaacgact gacgattacg ttcggcactg ttcatgttgc ctgccaggcc aagaaataac
    20101 gcgacagttg ttaatgggtt agctaacggc agtaacacca ccagccccag gccaattgct
    20161 ttaaacaaat ctaacattgg tggttgttat cctgtgtatc tgggttatca gcgaaaagta
    20221 taaggggtaa acaaggataa agtgtcactc tttagctaac cttgcatcgc attgaccaaa
    20281 acttgaaccg atttagcaaa acgtggcatc ggtcaattca tttatttgac ttatacttgc
    20341 ctgggcaata ttatcccctg caactaatta cttgccaggg caactaatgt gaaaagtacc
    20401 agcgatctgt tcaatgaaat tattccattg ggtcgcttaa tccatatggt taatcagaag
    20461 aaagatcgtc tgcttaacga gtatctgtct ccgctggata ttaccgcggc acagtttaag
    20521 gtgctctgct ctatccgctg cgcggcgtgt attactccgg ttgaactgaa aaaagtgttg
    20581 tctgtcgacc ttggagcact gacccgtatg ctggatcgcc tggtctgtaa aggctgggtt
    20641 gaaaggttgc cgaacccgaa tgataagcgc ggcgtactgg taaaacttac caccagcggc
    20701 gcggcaatat gcgaacaatg ccatcaatta gttggccagg acctgcatca agaattaaca
    20761 aaaaacctga cggcggacga agtggcaaca cttgagcatt tgcttaagaa agtcctgccg
    20821 taaacaaaaa agaggtatga cgatgtccag acgcaatact gacgctatta ccattcatag
    20881 cattttggac tggatcgagg acaacctgga atcgccactg tcactggaga aagtgtcaga
    20941 gcgttcaggt tactctaaat ggcacctgca acggatgttt aaaaaagaaa ccggtcattc
    21001 attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc tgaaggaaag
    21061 taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac aaactctgac
    21121 ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga ccaatatgca
    21181 gggcgaatcg cgctttttac atccattaaa tcattacaac agctagttga taacgtgaca
    21241 acgtcactga ggcaatcatg aaaccacttt catccgcaat agcagctgcg cttattctct
    21301 tttccgcgca gggcgttgcg gaacaaacca cgcagccagt tgttacttct tgtgccaatg
    21361 tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac atgggtactg
    21421 gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct atgtagttat
    21481 tgtcaggctc tgatcacaga gcctgacatc tccgtaatat cttcctcaag tctcaaatat
    21541 tttcgccatt gttggcgatg agatcccgat accagtaaaa cgacgcttta cgtatccgtt
    21601 ccagcgttcc cttcccttcg ttgtctttat cgacataaat cattccgtag cgctttttca
    21661 tttcccctgt tccggcagaa accaggtcaa tgcagcccca cggggtgtag ccaattaagt
    21721 caacaccatc ttcaactacg gcttttttca tttcccgaat atgggaagca aagtaatcaa
    21781 tgcgatagtg atcgttcacc gtgccgtcag cttgtctctg gtcaaccgca ccaaatccat
    21841 tttcgacaat aaacagcggc aactggaaat gatcccagaa ccagttgagt gaataacgta
    21901 gccctgccgg atcaatttgc catccccatt cggatgtatc gatataaggg ttgctgacca
    21961 aatctcgtgg ttcaacataa tccagttgcg ggttatcggg cgaaaattgt gtcgtaaaag
    22021 acatgtagta gctaaagccg ataaagtcga cacaaccact ggcaagaatc gcgttatctt
    22081 ctggtgtgat atcgaggttg aatcctttcc tggcaaagta attcagcata tgttgcggat
    22141 aatatccacg agcatgaaca tcagtaaacc agtaacgacg atgcattgct ttcgtggcca
    22201 tcatcatatc gttgggtgca cacgtcagag gatagatggg acacatggcg atcatacagc
    22261 caatgttaaa ttcaggattg atcgattttc cagtctgtac cgccagggca ctggcaacta
    22321 actcgtaatg taccgcctgg tacattattt gttcgcgctc attgatatct tcctctggcg
    22381 aatacaagat accggaatta gtaaatggac acaggctttc gctgaaattc acctgattat
    22441 tgatttcgtt aaacgtcatc cagtacttta ctttttcttt atagcgcgtg aagaccgttg
    22501 atgcgaagcg gataaaaaag tcgatcagtt tacggtttcg ccagccacca tattttgtca
    22561 ccagatgata aggcatctca aaatgtgaaa gcgtcaccac aggttccatt ccctgcttaa
    22621 ggcattcatc gaacagatca tcataaaatt gtaacccctc ttcattcggc tcctgttcgt
    22681 caccttgcgg aaagattcgt gtccagacaa tggaagttcg aaagcatttg aatcccatct
    22741 cggcaaataa ctgaatatct gttttatagc gatgataaaa atcaattgct tcatgattgg
    22801 gataattaag cccgtcgata acgccttctg tcacttcacg cggcaccccg tgagcgccag
    22861 cagtcatcac atcagcgatg ctgatgcctt ttcctccttc attccagcca ccttccaact
    22921 gatgcgcggc taccgcaccg ccccataaaa aacctttttt aaatcctgac atacacaact
    22981 ccttaactaa aatagattac caccagactt ccgcctggat gccggccata aactcgtctt
    23041 tgctgttctc attaaactta aaattcgata attcgttatc cagaatattg atatacgttc
    23101 catagaagcg aatttctggt cgtgaaccta aaatgctttc acccactttc aatgcatgcc
    23161 agagtgtcgt tttataagcc gattcattaa gtgttacacc ctgctgagtt ttgttctgtt
    23221 gcttaaacca gcctaattca agccccgtct gattccatgt attccagatc caggccggtc
    23281 ttattacagt gcgaatactg tcaaaatcac tatgagcacc actttcataa ctataaacat
    23341 cttcgccgtg agaatagaca agcgcgttag ccataataat attgtcagta agatacatct
    23401 cgccctgaga gattaaacgc caggcgatcc cattggtatg atcaccatag taatagcgac
    23461 catgcgccat cgtattactg gcatctgaga aactggcaaa actgctggca taggaattat
    23521 tggcaacctg taacgtaaat tcattaaacg tatcgcgttg caggttttgc cgtaaaacag
    23581 aagtcagcat ccagctatct ttcatttcaa aataactgtt gtcattttcg ttatcttgtt
    23641 gttgatccgt tttattaggt gcggaatatt tagccattaa tgataatgtc gcatcatccc
    23701 ataacgggat attgcgataa cgaacatcca cagaattggt attcatctgg ctggtacgcg
    23761 aaaaatcacg agagtaaaca tcgacatcat cacggcttaa ggacatatca aacagcccta
    23821 caccaagtgc ccagttttca atccccacac ccgcggcgac atccgtggtt aaggttttcc
    23881 agtccagcat ctggatctca tattgcggga gtttatgttt gccgacccag aagtctgcct
    23941 ctggtgcgaa gggtaaaaaa cctcgcgttg tcaggtaaat atcgctgaac tgcatgatat
    24001 tttcattggc actgtcacca aaccaggcat cgttatactg ctcacccacg ttgccatcat
    24061 aggtaacgac cgcatttgcc gttttaccgt cctgattata aacacgctga tttaaggtca
    24121 ggtcaaacca accgctcatc tcattaccaa aacgtcccag agaacccgct gcataagttt
    24181 gtggtgagcc gtgattacca gtaccccagc ctgagcggaa gtacccctga tagctgaacc
    24241 caatatcatc ctttatatat ttactgatat ctttcagggt cacgctttcg atagtggttt
    24301 tgtcgccttt actttcaata acggcaactt gctgcgatcc atttatagta tgcgctgcgg
    24361 taaacgagtt ctgttcaccg ttttcatttt tgatgttatc agcgacggga gatgtcgctg
    24421 atatttcagc aagcgaggct gatttatatt tgttttccgt gatgcttttt tgtaaggtcg
    24481 aaagtcggga tttatatact cccagttcat tctgtgttgc tttcagctct tgtttatttt
    24541 gcgacaattc attttcaagc aattcaaggc gctgctctat ggttaatttt gttgccatcg
    24601 cgttcaatgg aaacaatgct gtaataatag aaaaagacaa aaggctgacg ttaagcgtct
    24661 ttatattcat gacttatcct taagagtata ataatttcct ccagctatcg cactggagga
    24721 aaaaatcagg catggagttc ttttcttaat gaaataattt ccctggctaa atcctggcat
    24781 aatattgcat tcatcaaatg atcctgtgca tgaatcagaa ttaaattaac ttttattttg
    24841 ccacagcctt catcctgact aattaatgcg gtctgaatct tatgtgcctg aagacaggca
    24901 gattcagaac tggccattag ctgagttgcg ccctgccagt ctttttgccc ggcacaacgt
    24961 atggcttcca tcgcgtcaga acgtgcctgc cccgcgttga ccagtaactc cataacaagt
    25021 tcttcatctg caaacatcac aatctccttc ctgggtaaat ggaattatcc gttagttgtc
    25081 atgttattga ctttgttggc ggcaataaca aaaggtaaat agatcattgt cgccacacct
    25141 aaacataaaa aaccaaccag taaagccagc cagttccccc cggttccgag gaaagcaatt
    25201 aaaggtccgg gcgtagtcca gggcacggca taagcaaccg gtggaataat ttccatcgaa
    25261 acaaagaagt aaccaatggc acagttaaca atgggaacca taataaacgg caccatcatg
    25321 atggggttaa gtacaatagg aaggccaaac attatcggtt cattgatatt gaaaataccg
    25381 gggataaatg acatttttgc cagatcacgg tattccgcac gcttagaagc gataaaaata
    25441 gccaacaata accccaacgt catacctgaa ccgccgcagt tggcgaatgc atcattaata
    25501 gaagtccagg taatcggata tggcgcgccc caggtagtgc catgttgagc ggcccaggag
    25561 agattctcca gattagcctc agaaaaaacg gtttctcgaa ttgccgatgt ggtattaggt
    25621 ccgtggatcc ccagcaccca caggaaatta cccacagccc ccaggataat taccgcaaag
    25681 atattcgttc ccatatgttt gagcggcgtc tggattaatg tgtaaataag atcgtttagt
    25741 cctgccgggg atatcagtgt caggcaataa ttaagtgcgg aaaagaacac catgacaaaa
    25801 aatattggca ataaaacttt aaatgaacgc gctactgcag gcggtacagc tgccggcatc
    25861 gttatggtga tttttgggtt tcgagctaag cgacaaaata tttcactggt gatcaatgca
    25921 acgataacag caacaaacaa cccctgcgcg ccgagatatt tggtcgtcag gaatgcttgc
    25981 ccatctatca tctgatatgg cgtataaaca ataaaaaatg ctcctattgc cgttaatccg
    26041 cataaaagat catcttgctc atagctaatc gccatattcc tggcgaccag gaaagcaatc
    26101 ataattgaca tgatatttac agaaccattc attaccggag taaaaatagc ttgtgctttt
    26161 tcaaggttgg ggaaaatgct attcagatgc agtaatccgg caataaaacc gtctggcgat
    26221 aatatggcaa agttaattaa tataattaat gaacttgcca tgataattgg aaacgtaaaa
    26281 acgaatccat ctctcaccgc tgcaacatgt ttttgtgagt ttaacttgat agcaactgga
    26341 acaagaaaac gttccattcc acgttcgaat gaggccatta atcccatttt tttatacctg
    26401 ttgttttgga tatttataca tcccaataaa gcatgtatgc catatccatt ttatgatgtg
//
LOCUS       DAZGTR010000062        26343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGTR010000062 DAZGTR010000000
VERSION     DAZGTR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.062"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHLCY_002765"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHLCY_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP0369986.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHLCY_002766"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHLCY_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP0369987.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHLCY_002767"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHLCY_002767"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP0369988.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHLCY_002768"
     CDS             complement(1154..1621)
                     /locus_tag="ACHLCY_002768"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP0369989.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHLCY_002769"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHLCY_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP0369990.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHLCY_002770"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHLCY_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP0369991.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHLCY_002771"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHLCY_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP0369992.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHLCY_002772"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHLCY_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP0369993.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHLCY_002773"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHLCY_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP0369994.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHLCY_002774"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHLCY_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP0369995.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHLCY_002775"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHLCY_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP0369996.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHLCY_002776"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHLCY_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP0369997.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHLCY_002777"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHLCY_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP0369998.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHLCY_002778"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHLCY_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP0369999.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHLCY_002779"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHLCY_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP0370000.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHLCY_002780"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHLCY_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP0370001.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHLCY_002781"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHLCY_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP0370002.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHLCY_002782"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHLCY_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP0370003.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHLCY_002783"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHLCY_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP0370004.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHLCY_002784"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHLCY_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP0370005.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHLCY_002785"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHLCY_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP0370006.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHLCY_002786"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHLCY_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP0370007.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHLCY_002787"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHLCY_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP0370008.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHLCY_002788"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHLCY_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP0370009.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHLCY_002789"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHLCY_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP0370010.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHLCY_002790"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHLCY_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP0370011.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGCRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHLCY_002791"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHLCY_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP0370012.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHLCY_002792"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHLCY_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP0370013.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHLCY_002793"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHLCY_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP0370014.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHLCY_002794"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHLCY_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP0370015.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHLCY_002795"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHLCY_002795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP0370016.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHLCY_002796"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHLCY_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP0370017.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHLCY_002797"
     CDS             15761..16129
                     /locus_tag="ACHLCY_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP0370018.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHLCY_002798"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHLCY_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP0370019.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHLCY_002799"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHLCY_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP0370020.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHLCY_002800"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHLCY_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP0370021.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHLCY_002801"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHLCY_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP0370022.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRALQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHLCY_002802"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHLCY_002802"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP0370023.1"
                     /translation="MKKQRNLRSMAAQAVEQIVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGVYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQEELLAGFYASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHLCY_002803"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHLCY_002803"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP0370024.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHLCY_002804"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHLCY_002804"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP0370025.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22818
                     /gene="dprA"
                     /locus_tag="ACHLCY_002805"
                     /pseudo
     CDS             21710..22818
                     /gene="dprA"
                     /locus_tag="ACHLCY_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            22790..23263
                     /gene="smg"
                     /locus_tag="ACHLCY_002806"
     CDS             22790..23263
                     /gene="smg"
                     /locus_tag="ACHLCY_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP0370026.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23292..23834
                     /locus_tag="ACHLCY_002807"
     CDS             23292..23834
                     /locus_tag="ACHLCY_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP0370027.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23839..24411
                     /gene="tsaC"
                     /locus_tag="ACHLCY_002808"
     CDS             23839..24411
                     /gene="tsaC"
                     /locus_tag="ACHLCY_002808"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP0370028.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24416..25234
                     /gene="aroE"
                     /locus_tag="ACHLCY_002809"
     CDS             24416..25234
                     /gene="aroE"
                     /locus_tag="ACHLCY_002809"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP0370029.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25231..25488
                     /locus_tag="ACHLCY_002810"
     CDS             25231..25488
                     /locus_tag="ACHLCY_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP0370030.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25464..26018)
                     /locus_tag="ACHLCY_002811"
     CDS             complement(25464..26018)
                     /locus_tag="ACHLCY_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP0370031.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6763 a         6264 c         6832 g         6484 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg ttagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacaacagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggca atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaaatct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtagttcacc caacgttggc agaaattaag aaagaagggg agtctggttg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaacat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacaac gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgt catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag
    15841 cggcgaacca ttgatactgg atgatgattc tcatgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcagcgga gggaagcaat
    15961 tcagcttctg gatatcctga gaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtta aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggtcttct gctttcaggc gcttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgc cagtaatact tcttctttag ttggtgcagg tgcggcagct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaaatc gatcccgcca
    17101 attaataagc ccagaggagg catgatgata tcggcaacca gtgaagagac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggttgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg cccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg taccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttagcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcataaaacc cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgtta atcagcccct taagttgcgg gcgcttaatt
    19741 gcgacagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca caccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgtgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg atttgttcaa cggcctgtgc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gtaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga attcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgctagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata aagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 actgctttag gcagaattaa gccataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggctttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aaccgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatctagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagcccct ctgcttccag ttcaaatggt ttaccgtcgc
    21241 ggtcaagggc gcgaatttta actttctctg cgcgcggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc gtacatcgtc tcgaacatat
    21481 catcgacgat acgctgtatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatttgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcaaat tgatggattg cagcaaacag ggcttacatt
    21841 gcggcaggca caacgctttc tttcatttcc gcgaaagagt atcgaaagct cactttgttg
    21901 gttggagcaa cccaaccatc atttaatccc tgcggacagc gaattttatc ctcctcaact
    21961 tctggcgacg acagattacc ccggcgcact gtttgttgaa ggagaactgc acgcgctgca
    22021 ttcatttcag cttgccgtag tggggagtcg ggcgcattca tggtatggcg agcgatgggg
    22081 acggttattt tgcgaaactc tggcgacgcg tggagtgaca attacgagtg gactggcgcg
    22141 tggaatcgat ggtgtggcgc ataaagcggc cttacaggta aatggcgtca gcattgctgt
    22201 attggggaat ggacttaata ccattcatcc ccgccgccat gcccgactgg ctgccagtct
    22261 gcttgaacat ggcggagctc tcgtctcgga atttcccctc gatgttccac cccttgctta
    22321 caatttccca cgaagaaatc gcattatcag tggtctaagt aaaggtgtac tggtggtgga
    22381 agcggtggtt cgctggtgac agcacgttgt gcgcttgagc aggggcgtga agtttttgcc
    22441 ttgccaggtc caatagggaa tccgggaagc gaagggcctc actggttaat aaaacaaggt
    22501 gcgattcttg tgacggaacc ggaagatatt ctggaaaact tgcaatttgg attgcactgg
    22561 ttgccagacg cccctgaaaa ttcattttat tcaccagatc agcaagacgt ggcattgcca
    22621 tttcctgagc tcctggctaa cgtaggagat gaggtaacac ctgttgacgt cgtcgctgaa
    22681 cgtgccggcc aacctgtgcc agaggtagtt actcaactac tcgaactgga gttagcagga
    22741 tggatcgcag ctgtacccgg cggctatgtc cgattgagga gggcatgcca tgttcgacgt
    22801 actaatgtat ttgtttgaaa cctatattca cacagaagct gagttgcgtg tggatcaaga
    22861 caaacttgaa caggatctta ccgacgcagg atttgatcga gaagatatct acaatgccct
    22921 gctttggctg gaaaagcttg cggattatca ggaaggactg gcagaaccga tgcaactggc
    22981 ctctgatcca ctctccatgc gtatttatac ccctgaggag tgtgaaagac tggatgccag
    23041 ctgtcgtggt tttctgcttt tccttgagca aattcaggtg cttaaccttg aaacccgtga
    23101 aatggtgata gagcgagtgc tcgcgctgga taccgcagag ttcgacctgg aagacctgaa
    23161 atgggtaatc ctgatggtgt tgttcaatat tccgggctgc gaaaatgcgt accagcaaat
    23221 ggaagaatta ctctttgaag tgaatgaagg tatgctgcat taatcatttc tttaattcag
    23281 cataagttgt tatggcgaaa tcagcactgt tcacggtgcg taataatgag tcctgcccaa
    23341 agtgcggggc tgaactggtt attcgatccg ggaaacacgg tccgtttctt ggatgctcac
    23401 agtatccggc gtgtgactac gtccgtcctc tgaaatcttc agcggatgga catattgtca
    23461 aagttctgga ggggcaggtt tgccttgcat gtggcgcaaa tctggtatta cgccagggac
    23521 gctttggtat gtttattggt tgcagtaact accctgaatg cgaacatacc gaacttatcg
    23581 ataaaccgga cgaaacagca attacatgcc cccaatgccg gacgggccat ctggtccagc
    23641 gccgctcccg ttatggcaaa acatttcact cctgtgatcg ctacccggag tgtcaatttg
    23701 ccattaactt caaacccata gctggagaat gccctgagtg tcattatcca ctactcatcg
    23761 aaaagaaaac cgcgcagggt gtaaaacact tttgtgccag taaacaatgt ggaaagccgg
    23821 tttcggcgga ataataacgt gaataataac ctgcaaggag acgctatcgc agctgcgata
    23881 gatgtcctca atgaagaacg tgtcatcgcc tatccaacgg aagccgtttt cggtgttggg
    23941 tgcgatcctg atagcgaaac agcagtgatg agactgttgg agttaaaaca gcgtccggtt
    24001 gataaggggc tgattttaat cgcggcaaat tacgagcagc ttaaacccta tattgatgac
    24061 accatgctga ctgatgcgca gcgtgaaacc attttttccc gctggccagg tcctgtcacc
    24121 tttgtctttc ccgcgcctgc gacaacaccg cgctggttga cgggccgctt tgattcgctt
    24181 gctgtacgag tcaccgacca tccgttggtg gttgctttgt gccaggctta tggtaaaccg
    24241 ctggtttcta ccagtgccaa cttgagtgga ttgccacctt gtcgaacagt agacgaagtt
    24301 cgcgcacaat ttggcgcggc gttcccggtt gtgcctggtg aaacgggggg gcgtttaaat
    24361 ccttcagaaa tccgcgatgc cctgacgggt gaactgtttc gacaggggta acataatgga
    24421 aacctatgct gtttttggta atccgatagc ccacagcaaa tcgccattca ttcatcagca
    24481 atttgctcag caactgaata ttgaacatcc ctatgggcgc gtgttggcac ccatcaatga
    24541 tttcatcaac acactgaacg ctttctttcg tgctggtggt aaaggtgcga atgtgacggt
    24601 gccttttaaa gaagaggctt ttgccagagc ggatgagctt actgaacggg cagcgttggc
    24661 tggtgctgtt aataccctca agcggttaga agatggacgc ctgctgggtg acaataccga
    24721 tggtataggt ttgttaagcg atctggaacg tctgtctttt atccgtcctg gtttacgtat
    24781 tctgctcatc ggcgctggtg gagcatctcg cggcgtacta ctgccacttc tttccctgga
    24841 ctgtgcggtg acaatcacta atcggacggt atcccgcgcg gaagagttgg ctaaattgtt
    24901 tgcgcacact ggcagtattc aggcgttggg tatggacgaa ctggaaggtc atgagtttga
    24961 tctcattatt aatgcaacat ccagtggcat cagtggtgat attccggcga tcccgtcatc
    25021 gctcattcat ccaggcattt attgctatga catgttctat cagaaaggaa aaactccttt
    25081 tctggcatgg tgtgagcagc gaggctcaaa gcgtaatgct gatggtttag gaatgctggt
    25141 ggcacaggcg gctcatgcct ttcttctctg gcacggtgtt ctgcctgacg tagaaccagt
    25201 tataaagcaa ttgcaggagg aattgtccgc gtgaatcagg ccatccagtt tccggacaga
    25261 gaagagtggg acgagaataa aaaatgtgta tgttttcccg ctctcgtgaa tggcatgcaa
    25321 ctgacatgtg cgatctctgg cgagagtctg gcgtatcgtt ttactggaga tacgccagaa
    25381 cagtggttag cgagttttcg tcagcatcgc tgggatctgg aagaagaagc ggaaaactta
    25441 attcaggaac aaagtgaaga tgatcaaggc tgggtctggt taccctgatc cagatattcg
    25501 tccttccatt tcacgtaatt attcgcggaa tagcgtaacc cagccttctc ttcatcactt
    25561 aacgggcgga tctgtttgac ggggctaccg agatacagat atccgctctc cagccgttta
    25621 ttttgtggga ccagactacc cgcaccaatc atcacatcat cttctactat tgcgccatca
    25681 agtaaaattg agcccatccc aaccaaaact cgattgccaa tggtgcagcc gtggagcatc
    25741 accttgtgac caacagtgac atcttcgcca atggttaatg ggttgccatc tgggttgtac
    25801 gaggatttat gagtgacatg caacatactg ccatcctgga tattggtgcg tgctccgatc
    25861 tgtacataat gtacatctcc acgaatcaca acgagcggcc agatccccac atcatcagcc
    25921 agacgaacgt caccaatcac gacactgcta tcgtcgatca ttacgcgctg accgatttgt
    25981 ggaaaaagat cgcggtatgg gcgtaaaaca tcagacatac ttacctcagc aataaatgat
    26041 ttactaatga ctttgggggc attattggcc ttgtgcaagt cttttagtat gcaaaaaagc
    26101 accgttttgt gtgcgaatgc agcaaaaagg gtgaaaaaac aacaaacaga aaaaaagatc
    26161 aaaaaagtac ttgtgcaaaa aattgggatc cctataatgc gcctccgttg agacgacaac
    26221 gtgaaacact tcacaagatg gtcgtaacaa cgaagagaaa aaatcctgaa attaagggtt
    26281 gactctgaaa gaggaaagcg taatatacgc cacctcgcga cagtgagctg aaagccgcgt
    26341 cgc
//
LOCUS       DAZGTR010000063        25977 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGTR010000063 DAZGTR010000000
VERSION     DAZGTR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.063"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..193
                     /locus_tag="ACHLCY_002812"
     CDS             <1..193
                     /locus_tag="ACHLCY_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370032.1"
                     /translation="KAIRQDGLFALVKKMPDATLRVLYSHICQIQQRIRLPQLAHLHT
                     CSPHQKFILKLLNNHFPAN"
     gene            310..1608
                     /gene="kgtP"
                     /locus_tag="ACHLCY_002813"
     CDS             310..1608
                     /gene="kgtP"
                     /locus_tag="ACHLCY_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP0370033.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHLCY_002814"
     CDS             complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHLCY_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP0370034.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHLCY_002815"
     CDS             complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHLCY_002815"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP0370035.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDRVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHLCY_002816"
     CDS             complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHLCY_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP0370036.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHLCY_002817"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHLCY_002817"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP0370037.1"
                     /translation="MTENGVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHLCY_002818"
     CDS             complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHLCY_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP0370038.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHLCY_002819"
     CDS             7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHLCY_002819"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0370039.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHLCY_002820"
     CDS             complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHLCY_002820"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP0370040.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9606..9989
                     /gene="grcA"
                     /locus_tag="ACHLCY_002821"
     CDS             9606..9989
                     /gene="grcA"
                     /locus_tag="ACHLCY_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP0370041.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10045..10632)
                     /gene="eamB"
                     /locus_tag="ACHLCY_002822"
     CDS             complement(10045..10632)
                     /gene="eamB"
                     /locus_tag="ACHLCY_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP0370042.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHLCY_002823"
     CDS             10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHLCY_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0370043.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11827..13161)
                     /gene="srmB"
                     /locus_tag="ACHLCY_002824"
     CDS             complement(11827..13161)
                     /gene="srmB"
                     /locus_tag="ACHLCY_002824"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP0370044.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13293..14030
                     /gene="trmN"
                     /locus_tag="ACHLCY_002825"
     CDS             13293..14030
                     /gene="trmN"
                     /locus_tag="ACHLCY_002825"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP0370045.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14015..15637)
                     /gene="nadB"
                     /locus_tag="ACHLCY_002826"
     CDS             complement(14015..15637)
                     /gene="nadB"
                     /locus_tag="ACHLCY_002826"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP0370046.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15893..16048
                     /gene="rseD"
                     /locus_tag="ACHLCY_002827"
     CDS             15893..16048
                     /gene="rseD"
                     /locus_tag="ACHLCY_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP0370047.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16045..16620
                     /gene="rpoE"
                     /locus_tag="ACHLCY_002828"
     CDS             16045..16620
                     /gene="rpoE"
                     /locus_tag="ACHLCY_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP0370048.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16653..17303
                     /gene="rseA"
                     /locus_tag="ACHLCY_002829"
     CDS             16653..17303
                     /gene="rseA"
                     /locus_tag="ACHLCY_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP0370049.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17303..18259
                     /gene="rseB"
                     /locus_tag="ACHLCY_002830"
     CDS             17303..18259
                     /gene="rseB"
                     /locus_tag="ACHLCY_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP0370050.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18256..18735
                     /gene="rseC"
                     /locus_tag="ACHLCY_002831"
     CDS             18256..18735
                     /gene="rseC"
                     /locus_tag="ACHLCY_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP0370051.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKVSCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18933..20732
                     /gene="lepA"
                     /locus_tag="ACHLCY_002832"
     CDS             18933..20732
                     /gene="lepA"
                     /locus_tag="ACHLCY_002832"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP0370052.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20748..21722
                     /gene="lepB"
                     /locus_tag="ACHLCY_002833"
     CDS             20748..21722
                     /gene="lepB"
                     /locus_tag="ACHLCY_002833"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP0370053.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21995..22675
                     /gene="rnc"
                     /locus_tag="ACHLCY_002834"
     CDS             21995..22675
                     /gene="rnc"
                     /locus_tag="ACHLCY_002834"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP0370054.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22672..23577
                     /gene="era"
                     /locus_tag="ACHLCY_002835"
     CDS             22672..23577
                     /gene="era"
                     /locus_tag="ACHLCY_002835"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP0370055.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23589..24317
                     /gene="recO"
                     /locus_tag="ACHLCY_002836"
     CDS             23589..24317
                     /gene="recO"
                     /locus_tag="ACHLCY_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP0370056.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTCMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24329..25060
                     /gene="pdxJ"
                     /locus_tag="ACHLCY_002837"
     CDS             24329..25060
                     /gene="pdxJ"
                     /locus_tag="ACHLCY_002837"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP0370057.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25060..25440
                     /gene="acpS"
                     /locus_tag="ACHLCY_002838"
     CDS             25060..25440
                     /gene="acpS"
                     /locus_tag="ACHLCY_002838"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP0370058.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25860..25940
                     /gene="shoB"
                     /locus_tag="ACHLCY_002839"
     CDS             25860..25940
                     /gene="shoB"
                     /locus_tag="ACHLCY_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP0370059.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         6367 a         6486 c         6853 g         6271 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc gttggtgaaa aaaatgcctg atgcgacgct
       61 gcgcgtctta tactcccaca tatgccagat tcagcagcga atacggcttc cccaacttgc
      121 ccacctccat acgtgttctc ctcaccagaa atttatcctt aagctcctca ataaccattt
      181 tcctgctaac taaattcatg gttaaggttg cataatgata cgcaacaaat gtataacatt
      241 ttctttacca aaagaaataa acaaaagcga ccgacaaaag catcggatta cggcaggaga
      301 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgataca
      361 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      421 ttctatgtct actcgttctg ttcactctac ttcgcccaca ttttcttccc ttccgggaac
      481 acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct gatgcgccca
      541 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg
      601 ttatcggtgt gtatgatgtg tttcggatcg ctggttatcg cctgcctccc aggttatgaa
      661 actataggta cgtgggctcc ggcattattg cttctcgctc gtttatttca gggattatcc
      721 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      781 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact gctagcccta
      841 ctggttgtcg tggttttaca acacaccatg gaagacgctg cactcagaga gtggggatgg
      901 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcaa
      961 ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1021 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1081 tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1141 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1201 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1261 ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc ctcgccttat
     1321 gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac atcaatcagt
     1381 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggcctgtca
     1441 tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt gtcgctgaaa
     1501 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1561 ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg acgggtcagt
     1621 tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc cttccagctc
     1681 cagccgctcc cttcggggcg gctatcccaa agctcttttg acgcccccaa actgacagag
     1741 aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg tgaatattca
     1801 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1861 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga
     1921 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     1981 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag tttacgaacc
     2041 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2101 tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg tggatcgtgg
     2161 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2221 atcaacatcc acttatcatc aacccacatc ccttttaggt gataggtgtt gtcgtcatct
     2281 ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2341 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2401 tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2461 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtatggcgta
     2521 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt gttcaacaga
     2581 ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc ggcatcgccc
     2641 tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc gttcttgatt
     2701 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2761 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat aagatgataa
     2821 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     2881 taaagtactc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacgg
     2941 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcgg
     3001 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3061 tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc ttcatacaac
     3121 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3181 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3241 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3301 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3361 gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc cgattaagtg
     3421 gtcaacattt ccagtacctt actcatgatt cctcgcgttg ggcaagattt agcgtaagcc
     3481 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gctagcgcca
     3541 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg tgatctcgcg
     3601 tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg agatccgagc
     3661 gaaccagcac agcaaattcg gcatcgatgt tatcaggatc ggaaatcgca cgcgtgacgc
     3721 cgaggatctc ttccgtttga tcaatacgtc gtaccgctac aaacgccatt tcccgatcgt
     3781 agtcgatctg tgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3841 agcggtaata aagatcttct ttggtgactc gcgaaatgaa ttgctgaagt tgtggctcat
     3901 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccattctt
     3961 ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct tcaaacggcg
     4021 agatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4081 tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc accagaagct
     4141 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg tgcacgaatc tttttacttt
     4201 tgatcccctg aataaccaga tagcgggcca ggttcatgtt cagcggcggt aacgcgacga
     4261 cggcttgatc ttcaggacgc cactccacac cgccttcgcc cagcatgatc aacggcccga
     4321 aaaccggatc gtgctcaacc acaacccgca actcctgagc accagcacgg ttagccatac
     4381 tttgcaccaa caggccgtgg acccgtgcct gcggccaggc catttttacg cgatcgaaaa
     4441 tggcgtttgc cgcttgctgg acttcattgg ctgtacgcag gtagagcatg acgccctgaa
     4501 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4561 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtgggg agcgtgttca
     4621 tgccatatgc ttgcaggata ggctgaactt catgggtatc gagtgacgtt gctccttcgg
     4681 caatcgcctg ttgcaacaga agatgcgctt ctgcggtatt ggaggtcaga ttgctgggca
     4741 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4801 cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg aataatcgtc
     4861 gtgcctcttg cgaggaatgt tcgccacacc agttcgtcag cagagaaaca tacttgctgc
     4921 ggggatgatg ctttaccgct tcaattaata cttgcgcgct ttctgttgcg ggagcagcgg
     4981 cgctgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc agaatgtcca
     5041 gcgttttaat atagtgctca ctgctggcgt catcgcgcag atcgagcggg ttagagattg
     5101 ccacatgtcc tggcagtgca tcgcgcagtt tctggcaggt ttcttcgctt agtgttgcca
     5161 gcttgccatt gcgtgaccat aaggcatcaa gcgccagcgc ggcaggcgca gcaccgttgc
     5221 tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctaagggtt tccaccgccg
     5281 aaaacagttc gtgggtgtcc tgtacccgca acaaaccggc acgctgaata gccgcatccc
     5341 acgccggatc cattcctgcc gtggtgttca acagacgttg agccgccggg ctacgtccgc
     5401 ttttaatcac cagaatcggt ttgttgcgag aggcactacg ggccgccgaa acaaagcgtc
     5461 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgtgcca
     5521 gatagtcaag caattcatca acgtcgatat ccaggctgtc gccgagcgca ataaagtagg
     5581 aaaagcccat cttacgctgt tgcgcccagt cgaggatggt gttggagacg gcagccgatt
     5641 gcgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5701 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5761 gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata atgcaggttt
     5821 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     5881 aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     5941 acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6001 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgacgcgcca attaccgcta
     6061 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6121 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6181 gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa ccctgctgcc
     6241 gccccggtat cgctggccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6301 cctgcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc gacggaaatg
     6361 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6421 ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat cacttcccgt
     6481 tgctcatcag catacgaagc gggaaagacc accattggct gatagtccgg gttttgcacc
     6541 aactccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6601 aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6661 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6721 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6781 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacac cgttttcggt cataagagag
     6841 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     6901 attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg ccattaagca
     6961 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7021 gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7081 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7141 cccagaagtc gatcaccaca ggtagatcat ccttcagcaa tttgtcgagt gtttcaccgg
     7201 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7261 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7321 taactaacct cgggatgagt aaggagactt atcggcaacg acgccggtat gtttctaata
     7381 tgttacatat tatcggggac attgttccag acaacaatct gttttattca ataggtatga
     7441 tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7501 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7561 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7621 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7681 cgcgatgaca aacagagtca gccccgtgac cgcaagtggg aagattcgcc gtggcgcacg
     7741 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7801 aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7861 ggcgaaaatg catgtcaggc acttttccag agccgtccgg aagcgattgt tcgcgcctgg
     7921 tttatccaga gtgtaacgcc gcgttttaaa gaagccttgc gctggatggc agcaaaccgt
     7981 aaagcgtacc atgtggtgga tgaagcggaa ttgacaaaag cgtcaggcac ggaacatcac
     8041 ggcggcgttt gcttcttgat caaaaagcgt aacggtacaa ccgtgcagca gtgggtaagc
     8101 caggcaggcg cgcaggattg cgttctggca ctggaaaacg aatctaaccc gcataacctg
     8161 ggtggcatga tgcgcagctg cgcgcacttc ggcgtgaaag gtgtcgtggt gcaggatgcg
     8221 gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8281 ccgattactg gcgacaacat tgttaacgtg ctggatgatt tccgtcaggc aggttacacc
     8341 gtagtgacca cttccagcga gcagggtaaa ccgctgttca aaactagtct gccagcgaaa
     8401 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8461 gatctgcgcg tgaagattga cggtacgggc aacgttgccg ggctgaatat ctctgtcgca
     8521 accggcgttt tgcttggtga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8581 gcaactctgc catccggcat ttccccgcaa atttactcac tctctgccgg taatactggc
     8641 atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag cacaaaatgg
     8701 ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc tttcagtaca
     8761 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgtgatcc ccacaacaaa
     8821 aacaccacgc cttcgcggtg ctggttaatc aggctgatga ctttatcagt aaacgtctcc
     8881 caaccgaggc tggcgtggga atgcgcctga cctgcgcgaa ccgtcaacac agtattgagc
     8941 agcagaacgc cctgacgcgc ccagctttcg agatagccat gattagggcg ggtgaagccc
     9001 ggaatagtat tttccagctc tttatacata ttcaataatg acggaggaat ggcaatgccg
     9061 ggacgaacgg aaaatgccag accatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9121 agaatcacca ctttaacgtc acccaactcg gtaaagcgga acgcgttaaa gacatctttt
     9181 tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9241 agaaaataag gttgttgctt ctcttcagcc agcacgtcat gccaggttaa ttcgttagcc
     9301 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9361 aatcatgctc attttttaga cttaaacgag tgcaaaaaat gtaaattggt aaaatttcac
     9421 acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg atgtgtggtt
     9481 tttattgatt taaatcaaag aatcaagggt ggttgaggag tatatataca ctcaagcaac
     9541 aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg ccggggaggc
     9601 atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg ctgaactctt
     9661 tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa gcaggttatg
     9721 cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt gaagttccag
     9781 tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt aacgttctgc
     9841 gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag ctgaccatcc
     9901 gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag cgcgacgtta
     9961 tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta agcggaagag
    10021 ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat agaccagcaa
    10081 cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct gaaacagatg
    10141 ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca aaacgctgac
    10201 gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca gtgccgtaac
    10261 accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc aaaagctgat
    10321 tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc acgccagcca
    10381 gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt caatcactgc
    10441 cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca gactcatccc
    10501 tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa gggcgagaat
    10561 attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag cacttaaaag
    10621 ggtcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac ggaacattta
    10681 aaaaaaatga atgtttttat attctagatt cgatattttc gataggtttg gggttatgga
    10741 tctgcgccgt tttattacgc ttaaaaccgt ggtggaagag ggttcctttt tgcgagcttc
    10801 gcaaaaattg tgctgtacac aatcgacggt gacttttcat attcagcagc ttgagcagga
    10861 gttttcagtc cagttatttg agaaaattgg ccgacggatg tgccttaccc gcgaaggaaa
    10921 aaagttgctg ccgcacattt atgaacttac ccgagtgatg gatacgcttc gtgaggctgc
    10981 caaaaaggag tcagatccgg acggggaact acgcgttgtt tcgggcgaaa cgttgctctc
    11041 ttaccgaatg ccgcaggtgt tgcagcgttt tcggcaacgc gcaccaaaag tgcgtttatc
    11101 gttgcagtcg ctgaactgtt acgtgatccg tgatgcgcta ctgaatgatg aggccgatgt
    11161 cggcgtcttt tatcgcgtag ggaatgatga tgccctgaac cgacgagagt tgggtgaaca
    11221 atcgctggtg ctggtggctt caccgcaaat tgcagatgtc gattttactg agccgggaag
    11281 acataacgcc tgtagcttta ttatcaacga accgcaatgt gtcttccggc agatatttga
    11341 gagcacgctg cgtcagcggc ggatcacggt ggaaaacacc attgagctgt taagcattga
    11401 aagtatcaaa cgttgtgtcg cggcgaatat cggtgtcagt tacttgcccc gctttgcggt
    11461 ggaaaaagag cttgagagcg gagaattaat tgaattgcct tttggcgaac agtcgcagac
    11521 cattacggcc atgtgcgcac accatgctgg aaaagcggtt agcccggcga tgcacacttt
    11581 tattcagtgt gttgaagaga gttttgtggc tggataaaaa taatgtcggg attaaacccg
    11641 gcatttaact tgtcgacaaa gtgcgcttta ttcatgccgg atgcggcgtg aacgccttat
    11701 ccggcctaca agatcgtgca aattcaatat attgcatgag ccacgtagat actgataagc
    11761 gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt aaacccggca
    11821 ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt tacggcgctt
    11881 accaatattt ttggtgtcgc gatggcgttt tttcacacgc ggtttctctt tctctttagc
    11941 ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg tctgcttctc
    12001 gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa tcggctcttc
    12061 aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca gcgaaattgc
    12121 ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag tatcgccact
    12181 gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac cgcgcgcggc
    12241 aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga tcgcttcgtt
    12301 acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg cttcgcgcag
    12361 ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg agcgggtcgc
    12421 ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt catcggcgcg
    12481 gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag aaacctccac
    12541 cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca gcgttgccga
    12601 aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga tatcctgagc
    12661 gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa ccgcgcggca
    12721 atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga ccacgatgtc
    12781 ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg tgatggtggc
    12841 gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct gcatcgccag
    12901 ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac gcgggaaatc
    12961 gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc ctgtcggcgc
    13021 agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct gaatggcggt
    13081 cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga gttcaagttc
    13141 ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat agacgttacg
    13201 gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct cacccggtgc
    13261 tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc gtgcttcgtc
    13321 gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg atgaaagtgg
    13381 gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa cgttgccttg
    13441 atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc agtgacagcg
    13501 tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag gaaaatatca
    13561 accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag cagtggatca
    13621 cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat cagcagggag
    13681 tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt gatcacccat
    13741 cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc gtggtgctgc
    13801 cagagcagat tggtaatggt tttactgaac tggcattaag catgggctgg catttacgtt
    13861 tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg ctggcattct
    13921 ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca gaccagaact
    13981 attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa tgattgccgg
    14041 gggaaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc gtaaaatgca
    14101 gcccccggct ttctttgcgc atcatcgcgc agcgaacaat caactcggca acctgtacca
    14161 gattacgcag ctccagcaaa ttatttgaga cgcggaaatg ggcgtaatat tcgtctattt
    14221 cttgttggag catggttatc cgccgcaggg cgcgttccag gcgcttcgtt gtgcgcacaa
    14281 tgccaacgta atcccacata aacagccgta gctcgtgcca gttatgctga attactaccc
    14341 gttcgtcagg gttctcaacg cggctttcat cccacggcgg taacgtactg acgccgtggg
    14401 catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc agacactcca
    14461 gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc acctcgccaa
    14521 tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca ccaccgcagg
    14581 tataatgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc cccagcccga
    14641 gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc ttatggctga
    14701 tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg gcgcgggcga
    14761 caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa cgcgtgccat
    14821 ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg aaattgcgtg
    14881 cctgtgggtg atacagtgcg gtagggtgga actgattaaa ttcgagattg gcaacccgac
    14941 agcctgcgcg ccacgccata gcaatgccat cgccagaaga aatatccgga ttggtggtgt
    15001 actgataaac cttcgacgca ccgccggttg ccagtaccac tgcttttgcg tggcaggttt
    15061 ccaccgtttc tttatttcgg ttccataccc acgcgccaac aacccgtcgc gtgcccggga
    15121 ggccaatttt gtcagaaaca atcagatcaa ctgcgttgct gcgctccagc acacgaatat
    15181 tcggatggtt ctgcgccttg ctcaccagcg tggattgtac ttctctaccg gtggcgtcgg
    15241 cggcatgaag aatacgacgg tgactatgtc caccttcacg ggtcagatgg tagctttctt
    15301 cgccattcgg ttgaacgtgg gtatcaaaca aaaccccttg gtcaattaac cattgcacac
    15361 aggatcgtgc attgctggcg acaaattcaa ctgcatggcg atcgcaaata ccagccccgg
    15421 caatcaatgt gtcttccaca tgcgagtcaa tgctgtcagt ttcatcaaac acggcggcaa
    15481 taccgccctg ggcataaaat gttgaacctt ccgttaccgg gcctttactt agaacgatga
    15541 cctgatgctg gtcagccagg cgtagcgcca gtgaaagtcc ggctgcgccg ctaccgataa
    15601 tcaacacgtc acatgaatgt tctgggagag tattcatttt ctttgtttaa tttactaaac
    15661 accgtttggt cagcatagca tcatgttgtg cgaataaaca cctgctattt taatatttgt
    15721 tacagtggct aaacacgctg acgcagggcg gcgagaaaaa gagaagttac tggctggtgg
    15781 aggattaggt ggtgaaataa aaaggccgtt gggttactct tcaggcagtt aaatgggcat
    15841 ttctacacag ataatgcgat gttcagattc tgtagactta taatgataga taatgatccg
    15901 tctacagcat gacaaacaaa aacagatgcg ttacggaact ttacaaaaac gagacactct
    15961 aaccctttgc ttgctcaaat tgcagctaat ggagtggcgt ttcgatagcg cgtggaaatt
    16021 tggtttgggg agactttacc tcggatgagc gagcagttaa cggaccaggt cctggttgaa
    16081 cgggtccaga agggagatca gaaagccttt aacctactgg tagtgcgcta tcagcataaa
    16141 gtggcgagtc tggtttcccg ctatgtgccg tcgggtgatg ttcccgatgt ggtacaagaa
    16201 gcttttatta aagcctatcg tgcgctggat tcgttccggg gagatagcgc tttttataca
    16261 tggctgtatc ggattgctgt aaatacagcg aaaaattacc tggttgctca ggggcgtcgt
    16321 ccaccttcca gtgatgtgga tgccattgaa gctgaaaact tcgaaagtgg cggcgcgttg
    16381 aaagaaattt cgaaccctga gaacttaatg ttgtcagaag aactgagaca gatagttttc
    16441 cgaactattg agtccctccc ggaagatttg cgcatggcaa taaccttgcg ggagctggat
    16501 ggcctgagct atgaagagat agccgctatc atggattgtc cggtaggtac ggtgcgttca
    16561 cgtatcttcc gagcgaggga agctattgat aacaaagttc aaccgcttat caggcgttga
    16621 cgatagcggg atactggata agggtattag gcatgcagaa agaacaactt tccgctttaa
    16681 tggatggcga aacgctggat agtgagctgc ttaacgaact ggctcataac ccagaaatgc
    16741 agaaaacctg ggaaagctat cacttaatcc gtgactcaat gcggggtgat actcccgagg
    16801 tgctccattt cgatatctct tcacgcgtga tggccgccat tgaagaagag ccagtacgtc
    16861 aaccggcgac attgatcccg gaagcccagc ctgcgccgca tcaatggcag aaaatgccat
    16921 tctggcagaa agtacgtccg tgggcggcac agcttaccca aatgggcgta gcagcatgcg
    16981 tatcgcttgc agttatcgtt ggcgtccagc actataatgg acaatctgaa acgtcccagc
    17041 agcccgaaac gccggtattt aatacactgc cgatgatggg taaagccagc ccggtaagcc
    17101 tgggagtacc ttctgaagcg accgcaaaca atggtcaaca gcagcaggta caggagcagc
    17161 gtcgtcgcat taatgcaatg ttgcaggatt acgaactgca acgccgactc cactctgaac
    17221 agcttcagtt tgagcaggcg caaacccagc aagccgctgt acaggtgcca ggaattcaaa
    17281 ctttaggaac gcaatcgcag taatgaagca actttggttt gccatgtcat tagtgacagg
    17341 tagcctgtta ttctctgcta acgcctcggc cactcccgcg tccggggcgt tattacagca
    17401 gatgaacttg gccagtcagt cactgaatta cgagctgtca ttcatcagca tcaataaaca
    17461 gggtgttgag tctctgcgtt atcgacatgc acgcctcgat aaccgtcctc ttgcacaatt
    17521 gttgcaaatg gatggcccgc gccgggaagt ggtacagcgt ggcaatgaaa tcagctattt
    17581 tgaaccggga cttgaaccgt tcacgcttaa tggcgattac attgttgatt ctctgccatc
    17641 gctcatctat accgatttca aacgcctttc tccttactac gactttatct ccgtcgggcg
    17701 cacgcgtatt gctgatcgtc tttgcgaagt cattcgcgtg gttgcccgag atggtacacg
    17761 ctacagctac atcgtgtgga tggacatcga atcgaaatta ccgatgaggg ttgatctcct
    17821 tgatcgcgat ggtgaaacgc tggaacaatt tcgcgtgatt gcttttaacg tcaatcagga
    17881 tatcagtagc agtatgcaga cgctggcgaa ggcaaatttg ccgccgttgc tttctgttcc
    17941 tgtaggtgaa aaagctaaat tcagctggac gccaacctgg ttgccacagg gttttagcga
    18001 agtttccagt agtcgacgtc cgctaccgac gatggacaac atgcctatcg aatcacgtct
    18061 ctattccgac ggattattca gcttctcggt aaacgttaac cgcgctacgc catcgagcac
    18121 cgatcagatg ttgcgcaccg gacgcagaac cgtcagtaca agcgtacgtg ataacgccga
    18181 aatcaccatt gtcggtgaac tgccgccgca aacggcgaaa cgcattgccg agaatattaa
    18241 gttcggggca gcgcaatgat caaagagtgg gctaccgtcg tctcctggca aaacgggcag
    18301 gcgctggtca gttgtgatgt taaagtttca tgcagcagct gcgcttcacg cgccggttgc
    18361 ggtagccgcg tgttaaataa acttggcccg caaaccacgc ataccattgt cgtaccctgt
    18421 gatgagccgt tagtgccggg gcaaaaagtg gaattgggga tcgccgaagg cagcctgctt
    18481 agctccgcat tactggttta tatgtcgccg ctggtgggat tattcctcat cgcttcgcta
    18541 tttcagctac tctttgcttc tgacgtcgca gcattatgtg gtgcggtcct cggcggtatt
    18601 ggcgggttcc tgattgcgcg cggctactcg cgtaagtttg ctgcccgggc agaatggcaa
    18661 ccgatcatct taagcgtggc attgccgcct ggccttgtgc gatttgaaac atcttcggaa
    18721 gacgcgagcc agtgatttca ctggctttgt tgcattttgc ataacaaatg cgaggacgtt
    18781 tccagatacc acaatccata cggtatgaac atttcctcgc ctcaatgttg tagtgtagaa
    18841 tgcggcgttt ctattaatac agacgttaag ctcagaacag cgacttctaa aagcctggtt
    18901 aaccgggcat taaggcacaa taatcatact ttatgaagaa tatacgtaac ttttcgatca
    18961 tagctcacat tgaccacggt aaatcgacgc tgtctgaccg tattatccag atctgcggtg
    19021 gcctgtctga ccgtgaaatg gaggcgcagg ttctcgattc catggatctt gagcgtgagc
    19081 gtggcataac catcaaagcg caaagcgtga cgctggacta caaagcgtct gacggcgaaa
    19141 cctatcagct gaactttatc gacaccccgg gccacgttga cttctcctat gaagtttccc
    19201 gttcgctggc tgcctgtgaa ggtgcattgc tggtggtcga cgccgggcag ggcgtagaag
    19261 cgcaaaccct ggcaaactgc tacaccgcca tggaaatgga tctcgaagtg gtgccggtac
    19321 tgaacaagat tgacctgccg gcagccgatc ctgaacgcgt ggcggaagaa attgaagata
    19381 tcgtcggcat cgacgccacc gacgcggtgc gctgttcagc gaaaaccggc gttggcgtgc
    19441 aggacgttct cgaacgtctg gtgcgcgaca ttccgccgcc ggaaggcgat ccggaaggcc
    19501 cgttgcaggc actaattatc gactcatggt tcgacaacta cctgggcgtt gtttcactta
    19561 tccgtattaa aaacggcacc ctgcgtaagg gcgacaaagt gaaagtcatg agtaccgggc
    19621 agacctataa cgccgaccgt ctgggcatct tcacgccgaa acaggttgac cgcactgaac
    19681 tgaaatgtgg cgaagtaggc tggctggtat gtgcgattaa agatatccac ggcgctccag
    19741 tcggcgatac cttaacgctg gcgcgtaatc cggcagaaaa ggcgctgcct ggctttaaga
    19801 aagtcaaacc gcaggtatac gccggtctgt tcccggtaag ttccgacgac tatgaagcct
    19861 tccgtgacgc gctgggtaaa ctcagcctga acgatgcttc actgttctat gagccggaaa
    19921 gctccagcgc gctgggcttt ggtttccgct gcggcttcct cggcctgctg cacatggaga
    19981 tcatccagga acgtctggaa cgtgaatacg atctggatct gatcaccact gcgccgaccg
    20041 tagtgtatga agttgaaacc acgtcaagag aagttattta cgtcgacagt ccatccaagc
    20101 tgccagcggt aaataacatc tacgaactgc gcgagccgat tgccgaatgt cacatgctgc
    20161 tgccgcaggc atatctcggc aacgttatta cgctgtgcgt agaaaaacgc ggcgtgcaga
    20221 ccaatatggt ttaccacggt aatcaggtgg cgctgacgta cgagatcccg atggcggaag
    20281 tggtgctcga cttcttcgat cgcctgaaat ctacctcgcg tggttatgcg tctctggatt
    20341 acaacttcaa acgcttccag gcgtccgaca tggtacgtgt agacgtatta atcaacggtg
    20401 aacgtgttga tgcgttggcg ctgatcaccc accgtgataa ttcgcaaaac cgcggtcgcg
    20461 agttggtgga gaagatgaaa gatctgatcc cacgccagca gtttgatatc gccattcagg
    20521 cagcgattgg tacgcacatt attgcgcgat ccaccgtgaa acagctacgt aaaaacgtac
    20581 tggctaaatg ttatggcggc gatatcagcc gtaagaaaaa gctgctgcag aagcagaaag
    20641 aaggtaagaa acgcatgaag cagatcggta acgtcgagct gccgcaggaa gcgttcctcg
    20701 ccattctgca cgtcggcaaa gacaacaaat aacccttagg agttggcatg gcgaatatgt
    20761 ttgccctgat tctggtgatt gccacactgg tgacgggcat tttatggtgc gtggataaat
    20821 tctttttcgc acctaaacgg cgggaacgtc aggcagcggc gcaggcggct gccggtgact
    20881 cactggataa agcaaccctg aaaaaggttg cgccgaagcc tggctggctg gaaaccggtg
    20941 cttctgtttt tccggtgctg gctatcgtat tgattgtacg ttcgtttatt tatgaaccgt
    21001 tccagatccc gtcaggttcg atgatgccga ctctgttaat cggtgatttt attctggtag
    21061 agaagtttgc ttatggcatt aaagatccta tctaccagaa aacgctgatc gaaaccggtc
    21121 atccgaaacg cggcgatatc gtggtcttta aatatccgga agatccaaag cttgattaca
    21181 tcaagcgcgc ggtgggttta ccgggcgata aagtcactta cgatccggtc tcaaaagagc
    21241 tgacgattca accgggatgc agttccggcc aggcgtgtga aaacgcgcta ccggtcacct
    21301 actccaacgt ggaaccgagc gatttcgttc agaccttctc acgccgtaat ggtggggaag
    21361 cgaccagcgg attctttgaa gtgccgaaaa acgaaaccaa agaaaatgga attcgtcttt
    21421 ccgagcgtaa agagacactg ggtgatgtga cgcaccgaat tctgacagtg ccgattgcgc
    21481 aggaccaggt ggggatgtat taccagcagc cagggcaaca actggcaacc tggattgttc
    21541 cgccgggaca atacttcatg atgggcgaca accgcgacaa cagcgcggac agccgttact
    21601 ggggttttgt gcctgaagcg aatctggtcg gtcgggcaac ggctatctgg atgagtttcg
    21661 ataagcaaga aggcgaatgg ccgacaggtg tgcgcttaag tcgcattggc ggcatccatt
    21721 aatagcccat cttcgttcac gttgtcgccg ttatggcgac aacgtgaatt atttatgaga
    21781 taaatctccc gtggctaacg acatcccccg tcgttgtgta tagaatattc ccccgaagtt
    21841 taaggttggc acctccaggt tgccacggca cacgaaacag cgttggtccc catataccgg
    21901 taaactgaaa ctgcagcgaa gcagttagca gagccatgta tatcaggtct gtttcgtgtg
    21961 ctgaattgtt gacgcattta tttattggta tcgcatgaac cccatcgtaa ttaatcggct
    22021 tcaacggaag ctgggctaca cttttaatca tcaggaactg ttgcagcagg cattaactca
    22081 tcgtagtgcc agcagcaaac ataacgagcg tttagaattt ttaggcgact ctattctgag
    22141 ctacgttatc gccaatgcgc tttatcaccg tttccctcgt gtggatgaag gcgatatgag
    22201 ccggatgcgc gccacgctgg tccgtggcaa tacgctggcg gaactggcgc gcgaatttga
    22261 gttaggcgag tgcttacgtt tagggccagg tgaacttaaa agcggtggat ttcgtcgtga
    22321 gtcaattctc gccgacaccg tcgaagcatt aattggtggc gtattcctcg acagtgatat
    22381 tcaaaccgtc gagaaattaa tcctcaactg gtatcaaact cgtttggacg aaattagccc
    22441 aggcgataaa caaaaagatc cgaaaacgcg cttgcaagaa tatttgcagg gtcgccatct
    22501 gccgctgccg acttatctgg tagtccaggt acgtggcgaa gcgcacgatc aggaatttac
    22561 tatccactgc caggtcagcg gcctgagtga accggtggtt ggcacaggtt caagccgtcg
    22621 taaggctgag caggctgccg ccgaacaggc gttgaaaaaa ctggagctgg aatgagcatc
    22681 gataaaagtt actgcggatt tattgccatc gtcggacgtc cgaacgttgg caaatccaca
    22741 ttgttgaaca aactgctggg gcagaagatc tccatcacct cccgcaaagc gcaaacaact
    22801 cgtcaccgca ttgtggggat ccatactgaa ggcgcgtatc aggcgatcta cgttgatacc
    22861 cctggcctgc atatggaaga aaaacgcgct attaaccgcc tgatgaacaa agcggcgagc
    22921 agctctattg gcgacgttga gctggtgatt tttgtcgttg aaggcacccg ctggacgccg
    22981 gacgacgaaa tggtgctcaa caaactgcgc gatggcaaag cgccggtgat cctcgcggtg
    23041 aacaaagtgg ataacgtgca ggaaaaagcc gatctgctgc cgcacttaca gttcctggca
    23101 agtcagatga acttcctcga tatcgtgccg atctctgctg aaaccgggct gaatgtcgac
    23161 actatcgcag caatcgtgcg taagcatctc cctgaagcga cccatcactt cccggaagat
    23221 tacatcaccg atcgctcaca gcgttttatg gcgtctgaaa tcatccgcga aaaactgatg
    23281 cgtttcctcg gcgctgaact gccgtactcc gtgaccgtgg agatcgaacg tttcgtctct
    23341 aacgaacgcg gtggttatga catcaatggt ttgattctcg ttgagcgtga agggcagaag
    23401 aagatggtta ttggcaacaa aggggccaag atcaaaacca tcgggattga agcgcgtaaa
    23461 gacatgcagg aaatgttcga agcgcctgtc caccttgaac tgtgggtaaa agtgaaatcc
    23521 ggttgggccg acgacgaacg cgcactgcgc agtctcggtt acgttgacga tctttaagag
    23581 tcactccgat ggaaggctgg cagcgcgcat ttgtcctgca tagtcgcccg tggagcgaaa
    23641 ccagcctgat gctggacgtc ttcacggagg aatcgggtcg cgtgcgtctg gttgccaaag
    23701 gcgcacgctc taaacgctct actctgaaag gtgcattaca gcctttcacc cctctcttgc
    23761 tacgtttcgg cgggcgtggc gaagtcaaaa cgctgcgcag tgctgaagct gtctcgctgg
    23821 cgctgccatt aagcggtatc acgctttaca gcggtctgta catcaacgaa cttctctccc
    23881 gcgtactgga atacgagacg cgcttctctg aactcttttt cgattacttg cactgcattc
    23941 agtctcttgc aggtgccact ggtacgccag aacccgcgct gcgccgcttt gaactggcac
    24001 tgctcgggca tctgggctat ggcgtcaatt ttacccattg tgcgggtagc ggcgagccgg
    24061 tagatgacac catgacgtat cgttatcgcg aagaaaaagg gtttatcgca agcgtcgtta
    24121 tcgacaataa aacgttcacc ggaaggcagt taaaagcgtt aaacgcgcgg gaatttcctg
    24181 acgcagacac actacgcgcc gcgaaacgct ttacctgcat ggcgcttaag ccgtatcttg
    24241 gcggtaaacc attaaagagc agggaactgt tccggcagtt tatgcctaag cgaacggtga
    24301 aaacacatta tgaatgatga ggattgtcat ggctgaatta ctgttaggcg tcaacattga
    24361 ccatattgca acgctgcgta acgcacgcgg tactgcttac ccggatccgg tgcaggcggc
    24421 gtttattgct gagcaggcgg gagcggacgg cattaccgtg catttacgtg aagatcgccg
    24481 ccacattact gaccgtgatg tgcgcatcct gcgtcagacg ctggataccc gcatgaatct
    24541 ggagatggcg gtgaccgaag agatgctggc gatcgccgtt gagacgaagc cacatttttg
    24601 ctgtctggta ccggaaaagc gtcaggaagt aacaaccgaa ggcggcctgg acgtcgcagg
    24661 gcagcgtgac aaaatgcgcg atgcctgcaa acgtctggca gatgccggga tccaggtttc
    24721 tctctttatc gacgccgatg aagagcagat caaagctgcg gcagaggttg gcgcaccgtt
    24781 tatcgagatc cacaccggtt gctatgctga tgccaaaact gacgccgaac aggcgcaaga
    24841 gctggcgcgt atcgccaaag ccgcgacctt tgccgctagt ctcggtctga aagttaacgc
    24901 tggtcacggt ctgacctatc acaacgtgaa agctattgcc gccatccctg agatgcatga
    24961 actgaatatc ggtcatgcca ttattggtcg tgcagtgatg accggactga aagatgcggt
    25021 ggcagaaatg aagcgtctga tgctggaagc gcgtggctaa tggcaatatt aggtttaggc
    25081 acggatatcg tagagatcgc tcgcatagaa gcagtgatcg cccgatccgg tgagcgcctg
    25141 gcacgccgcg tattaagcga taacgaatgg gcaatctgga aaacgcacca ccagccggtg
    25201 cgttttctgg cgaagcgttt tgctgtgaaa gaagccgcag caaaagcgtt tggcaccggg
    25261 atccgcaatg gtctggcgtt taatcaattt gaagtattca atgatgagct cggcaaacca
    25321 cggctacggc tatggggtga ggcattaaaa ctggcggaaa agctcggcgt tgtaaatatg
    25381 catgtaacgc tggcagatga acgccactat gcttgtgcca cggtaattat tgaaagttaa
    25441 aataataccg ttctctttaa aaagtaaaac ccccgaactg gtaaggaacg ggggttttac
    25501 tttttaactt taattttgtg cagcatgcac cctcaacaac agcgattatg gatcagtgta
    25561 cttatctttt acaagcaact tgcaatcttt agcataaaaa ctcgagcctt tacgaagaaa
    25621 gcaatattga tggaaagatt aacgtgaccg ccaattcgta agtacattaa aattggcttc
    25681 gttattgaag attttgctgt gctttacacc atgccacaga attcccccat tgaaacgagt
    25741 ggtgtcgtca aagctctggt gtggagtgca gcatgcaccc tcaataactc gcacgttcag
    25801 ttttggggag atgtaagggc taatctgaac ggctgcattc cttgtttaag gaaaaacgaa
    25861 tgactgattg ccgatacctg attaaacggg tcatcaaaat catcattgct gttttacagc
    25921 tgatccttct gttcttataa cacaaggaaa cgtacttaag gtgcgtccgg tgaacca
//
LOCUS       DAZGTR010000064        25709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGTR010000064 DAZGTR010000000
VERSION     DAZGTR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.064"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHLCY_002840"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHLCY_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP0370060.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHLCY_002841"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHLCY_002841"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP0370061.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHLCY_002842"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHLCY_002842"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP0370062.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHLCY_002843"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHLCY_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP0370063.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHLCY_002844"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHLCY_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP0370064.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHLCY_002845"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHLCY_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP0370065.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHLCY_002846"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHLCY_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP0370066.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHLCY_002847"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHLCY_002847"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP0370067.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQMRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHLCY_002848"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHLCY_002848"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP0370068.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHLCY_002849"
     CDS             9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHLCY_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP0370069.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHLCY_002850"
     CDS             10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHLCY_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP0370070.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHLCY_002851"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHLCY_002851"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP0370071.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHLCY_002852"
     CDS             12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHLCY_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0370072.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHAHMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHLCY_002853"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHLCY_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP0370073.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHLCY_002854"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHLCY_002854"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP0370074.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPLVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHLCY_002855"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHLCY_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP0370075.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHLCY_002856"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHLCY_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP0370076.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHLCY_002857"
     CDS             complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHLCY_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP0370077.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTIKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHLCY_002858"
     CDS             16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHLCY_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP0370078.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHLCY_002859"
     CDS             17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHLCY_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP0370079.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHLCY_002860"
     CDS             complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHLCY_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP0370080.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19444..20322
                     /gene="nadK"
                     /locus_tag="ACHLCY_002861"
     CDS             19444..20322
                     /gene="nadK"
                     /locus_tag="ACHLCY_002861"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP0370081.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20408..22069
                     /gene="recN"
                     /locus_tag="ACHLCY_002862"
     CDS             20408..22069
                     /gene="recN"
                     /locus_tag="ACHLCY_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP0370082.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22218..22559
                     /gene="bamE"
                     /locus_tag="ACHLCY_002863"
     CDS             22218..22559
                     /gene="bamE"
                     /locus_tag="ACHLCY_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0370083.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHLCY_002864"
     CDS             complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHLCY_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP0370084.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHLCY_002865"
     CDS             complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHLCY_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP0370085.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23509..23991
                     /gene="smpB"
                     /locus_tag="ACHLCY_002866"
     CDS             23509..23991
                     /gene="smpB"
                     /locus_tag="ACHLCY_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP0370086.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHLCY_002867"
     tmRNA           24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHLCY_002867"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24840..25088
                     /locus_tag="ACHLCY_002868"
     CDS             24840..25088
                     /locus_tag="ACHLCY_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0370087.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            complement(25099..25170)
                     /locus_tag="ACHLCY_002869"
                     /pseudo
     CDS             complement(25099..25170)
                     /locus_tag="ACHLCY_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(25456..>25709)
                     /locus_tag="ACHLCY_002870"
     CDS             complement(25456..>25709)
                     /locus_tag="ACHLCY_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0370088.1"
                     /translation="LMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQD
                     RVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT         6558 a         6796 c         6024 g         6331 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa agcatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagca ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ctattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcatc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacactcc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcgtcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ccgcccccat cattcagccg attcgtcaca
    11401 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tagaagcgct gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaat
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcggaaa aattacaaga gcttgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccatatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccgcgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattact accccttaaa
    12721 acaccagcaa acagataaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tagcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tcatttttgc gggaattgca atcaacagtt gctaactctg ctgtaaaagg ccgtcggcgg
    14941 tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag cgtacacgca gtacgtgagg
    15001 atgacgagca catcccggtg ccaaaatggc aaacaagcca ggccgattag cgaccaggga
    15061 agcctggggg catcataccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
    15121 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggttc acgtcctgca
    15181 cctgcatacc gcaaccggca gcgatacgac gcttacgcga acctttgatg atttctggct
    15241 tagcgcgctc ttttatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
    15301 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
    15361 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
    15421 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
    15481 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
    15541 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
    15601 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
    15661 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
    15721 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
    15781 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
    15841 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
    15901 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    15961 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
    16021 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
    16081 cttgcaggcc cgccatcagt acgaccgcag gcggttgcgc agccaggttc agggtctggt
    16141 tctcttcgcc catcgccgca accagttcgt tacggactat tttgacgaac tcctgccccg
    16201 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    16261 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    16321 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    16381 acaaacgatc agttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    16441 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    16501 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    16561 ctgtcaacat tactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    16621 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    16681 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    16741 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
    16801 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    16861 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    16921 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
    16981 ccacgctgat aattgccgcc ctgtacgcgc tacaactggc gtggattgat taccaactga
    17041 agaacaagaa gctggcgttt aaccaagaaa tgccgccatt gatgagtatc gagcgtaaaa
    17101 tgttccacat cacgcagatt ggcgtggtgc tactaacgct cacgctttgc actggcctgt
    17161 tctacatgca caacttattt agcatggaaa atatcgacaa ggccgtgctc tctatcgtgg
    17221 cgtggtttgt ctatattgtg ctgctgtggg gacattatca tgaaggatgg cgtggacgcc
    17281 gcgtcgtctg gtttaacgtt gcgggtgcag tcatcctgac actggcctac ttcggcagcc
    17341 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    17401 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    17461 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
    17521 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    17581 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
    17641 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    17701 actggttttt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    17761 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    17821 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
    17881 tagcggctct ttgagcaaag aagagttgcg cactatcgtg cacgaatcac gctcacaaat
    17941 atcccgtcgc aatcaggata tgctactgtc tgtgctcgat ctggaaaaga tgaccgttga
    18001 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    18061 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
    18121 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
    18181 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgccggaagg
    18241 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
    18301 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    18361 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    18421 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
    18481 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
    18541 agagatccct gtcgcaggca cccgcgtgcg tattggcgag tacgatatag atattctcga
    18601 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
    18661 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    18721 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    18781 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    18841 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    18901 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    18961 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    19021 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    19081 caatatccag ttcagtacgt cgacgcaggt tttccatttc ggcttttaca cgcaaaatgc
    19141 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    19201 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    19261 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    19321 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    19381 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    19441 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccccactgca
    19501 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    19561 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    19621 attgggcaac tagctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    19681 gcacgcacac tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    19741 ttcctgactg accttgaccc cgataacgcc cagcaacagt tagccgatgt gctggaaggc
    19801 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccag
    19861 aaacgcatca gcaccgcgat aaatgaagtg gtgcttcatc caggcaaagt ggcgcatatg
    19921 attgagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatggacta
    19981 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcaggcgg tcctattctg
    20041 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    20101 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    20161 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    20221 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaac
    20281 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    20341 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    20401 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    20461 tgattttcat agcggcatga ccgtaataac tggcgagacc ggcgcgggta aatctattgc
    20521 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    20581 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggctctgcg
    20641 ctggctggaa gaaaatcagc ttgaagacgg gcatgaatgt ttgcttcgtc gagtgatcag
    20701 cagcgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    20761 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcaat tactcaccaa
    20821 acctgagcac caaaaattcc tgcttgatgg ctatgccaat gaaacctctc tactgcagga
    20881 aatgaccgca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    20941 gttaagtcag gaacgcgccg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    21001 cgaatttaat ccgcagcccg gagagtttga acaaatcgac gaagagtaca aacgtctggc
    21061 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    21121 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    21181 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    21241 tgaagccagc gatgaactgc gccactactg cgatcgtctg gatctcgatc ccaaccgact
    21301 atttgaactt gaacagcgca tctcgaaaca gatttcgctg gcacgtaaac atcacgtcag
    21361 ccctgaggca ttgccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    21421 tcaggccgac tcacaagaaa cgcttgcgct ggcggtaacg aaacatcatc agcaggcact
    21481 ggaaatcgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    21541 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga tcgatgttaa
    21601 atttgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    21661 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattgtcccg
    21721 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    21781 tgatgaagtg gatgtaggga ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    21841 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    21901 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacgcatat
    21961 gcaatccctg aataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    22021 agtcacacgt aatacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    22081 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    22141 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    22201 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    22261 tgttgaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    22321 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    22381 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    22441 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    22501 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    22561 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    22621 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    22681 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    22741 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    22801 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcacgc gctgcaggta
    22861 ctgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtaaacct
    22921 ctttcgcacg aaccgtaaaa gcctggacca tattagacgc cagctcttta aacacgcgac
    22981 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    23041 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    23101 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    23161 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    23221 gaatccgact tccggtacaa cccggcaaaa actgaggata agactgaacg tcattcacta
    23281 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    23341 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    23401 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    23461 tgatatgggg tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    23521 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    23581 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg
    23641 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    23701 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    23761 acccgtaccc gcaagttact tctcaaccag cgcgaactgg actcattgta cggtcgcgtc
    23821 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    23881 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    23941 cgcgaatggc aggtggataa agcacgtatc atgaaaaacg cccaccgtta aacctgcact
    24001 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    24061 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    24121 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    24181 tctggtatac ttgcctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    24241 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    24301 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    24361 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    24421 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    24481 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    24541 gcgggttcaa ctcccgccag ctccaccact ttaagaagga ctacaaccgg acagtagcaa
    24601 taaatacagc cacttacgga cacaagccag acagtagtcg gactgaaaaa agacaaaaat
    24661 atacacgtga aatgcacgtg cacttctaaa aacccctagt tatcaccgac tggggttttt
    24721 caatttgtaa ctaagggtaa caaaaacacc cccgtttttt tcatctcact caccttgata
    24781 ctgtttattt gtacagttaa aaataatact gtatacaaac acagtacaga gggactttta
    24841 tgcgtattga gatctgcata gccaaagaaa aaatgactaa aatgccaaac ggtgctgtgg
    24901 atgcgttaaa ggaagaatta acccgacgca tcagtaaacg ttatgacgat gtagaggtga
    24961 tcgtaaaagc caccagcaac gatggccttt ctgttacgcg caccgccgat aaagattctg
    25021 caaaaacttt tgttcaggag acgctgaaag atacctggga gtctgctgac gagtggtttg
    25081 ttcgctaata aacacgtaaa tcggtaacgg ctggaaatca ttcaatactc gcactatcga
    25141 aagttcacca gccaaccgcg acacgctctt acatacgaag tgccgcgctt tccttaataa
    25201 ttttttagca gtactgtgta aataatgagc gacctaatcc atgcatgacg cgtagtgcct
    25261 attgtgcatc tttctgcgtc tctttttact ggacccaagc caatgatgct ggcgatacca
    25321 ttcccgccag gcccacttac aacagtaaaa ttcggagctc tgcctttaca tacaatacaa
    25381 tctacctaac aaatatcccc cggacattgc aacacaaaaa ccggagccgg actccggttt
    25441 tgtgaagctg tcgggttact tcatcccgcc aatattttcc cacctcccgt cagcacgcag
    25501 gatttgcagc ggtcttacca cgcactgtat ctgcttttta tccgcatcca gtatcaccac
    25561 ctgcgtgatt accctgtcct gctccgggat aatgccattc tcatctgact ccaggatgtc
    25621 tgccggcccc agtcgcagtt gtgctgtaag cgactgcacg tgttcacggc catcatgctt
    25681 tccgcaacca cacagacgct gcataagtt
//
LOCUS       DAZGTR010000065        25646 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGTR010000065 DAZGTR010000000
VERSION     DAZGTR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.065"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            396..1262
                     /gene="folD"
                     /locus_tag="ACHLCY_002871"
     CDS             396..1262
                     /gene="folD"
                     /locus_tag="ACHLCY_002871"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP0370089.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1264..1476
                     /gene="ybcJ"
                     /locus_tag="ACHLCY_002872"
     CDS             1264..1476
                     /gene="ybcJ"
                     /locus_tag="ACHLCY_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP0370090.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1584..2105
                     /locus_tag="ACHLCY_002873"
     CDS             1584..2105
                     /locus_tag="ACHLCY_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0370091.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHREFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2141..3526)
                     /gene="cysS"
                     /locus_tag="ACHLCY_002874"
     CDS             complement(2141..3526)
                     /gene="cysS"
                     /locus_tag="ACHLCY_002874"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP0370092.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYIALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3700..4194
                     /gene="ppiB"
                     /locus_tag="ACHLCY_002875"
     CDS             3700..4194
                     /gene="ppiB"
                     /locus_tag="ACHLCY_002875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP0370093.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4197..4919
                     /gene="lpxH"
                     /locus_tag="ACHLCY_002876"
     CDS             4197..4919
                     /gene="lpxH"
                     /locus_tag="ACHLCY_002876"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP0370094.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5037..5546
                     /gene="purE"
                     /locus_tag="ACHLCY_002877"
     CDS             5037..5546
                     /gene="purE"
                     /locus_tag="ACHLCY_002877"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP0370095.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5543..6610
                     /gene="purK"
                     /locus_tag="ACHLCY_002878"
     CDS             5543..6610
                     /gene="purK"
                     /locus_tag="ACHLCY_002878"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP0370096.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPRLPPEYASGVMWAQSKFS"
     gene            complement(6747..7640)
                     /locus_tag="ACHLCY_002879"
     CDS             complement(6747..7640)
                     /locus_tag="ACHLCY_002879"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0370097.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7637..8452)
                     /locus_tag="ACHLCY_002880"
     CDS             complement(7637..8452)
                     /locus_tag="ACHLCY_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0370098.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8463..9722)
                     /locus_tag="ACHLCY_002881"
     CDS             complement(8463..9722)
                     /locus_tag="ACHLCY_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0370099.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9732..11399)
                     /locus_tag="ACHLCY_002882"
     CDS             complement(9732..11399)
                     /locus_tag="ACHLCY_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0370100.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11716..12765
                     /gene="allD"
                     /locus_tag="ACHLCY_002883"
     CDS             11716..12765
                     /gene="allD"
                     /locus_tag="ACHLCY_002883"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP0370101.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12787..14022
                     /gene="allC"
                     /locus_tag="ACHLCY_002884"
     CDS             12787..14022
                     /gene="allC"
                     /locus_tag="ACHLCY_002884"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP0370102.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            14033..14818
                     /gene="allE"
                     /locus_tag="ACHLCY_002885"
     CDS             14033..14818
                     /gene="allE"
                     /locus_tag="ACHLCY_002885"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP0370103.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14947..16092)
                     /gene="glxK"
                     /locus_tag="ACHLCY_002886"
     CDS             complement(14947..16092)
                     /gene="glxK"
                     /locus_tag="ACHLCY_002886"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP0370104.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGETVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLRAV
                     NLEQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16114..17415)
                     /locus_tag="ACHLCY_002887"
     CDS             complement(16114..17415)
                     /locus_tag="ACHLCY_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP0370105.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17472..18833)
                     /gene="allB"
                     /locus_tag="ACHLCY_002888"
     CDS             complement(17472..18833)
                     /gene="allB"
                     /locus_tag="ACHLCY_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP0370106.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(18893..20347)
                     /locus_tag="ACHLCY_002889"
     CDS             complement(18893..20347)
                     /locus_tag="ACHLCY_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP0370107.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLIFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENGGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIHFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20516..21394)
                     /gene="glxR"
                     /locus_tag="ACHLCY_002890"
     CDS             complement(20516..21394)
                     /gene="glxR"
                     /locus_tag="ACHLCY_002890"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0370108.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21494..22270)
                     /gene="hyi"
                     /locus_tag="ACHLCY_002891"
     CDS             complement(21494..22270)
                     /gene="hyi"
                     /locus_tag="ACHLCY_002891"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP0370109.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPQTTTEAGLRWMDPYR"
     gene            complement(22283..24064)
                     /gene="gcl"
                     /locus_tag="ACHLCY_002892"
     CDS             complement(22283..24064)
                     /gene="gcl"
                     /locus_tag="ACHLCY_002892"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP0370110.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24154..24969)
                     /gene="allR"
                     /locus_tag="ACHLCY_002893"
     CDS             complement(24154..24969)
                     /gene="allR"
                     /locus_tag="ACHLCY_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP0370111.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(25047..25529)
                     /gene="allA"
                     /locus_tag="ACHLCY_002894"
     CDS             complement(25047..25529)
                     /gene="allA"
                     /locus_tag="ACHLCY_002894"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP0370112.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
BASE COUNT         6317 a         7027 c         6342 g         5960 t
ORIGIN      
        1 tttttatcct ctaagaatgt cttaattgaa aatatgcact ctattctaaa aaatagagag
       61 ccccgttaga tgaatacttt cgcgcaaaat atattcaaca caaatataga cctgaagcgg
      121 taaattacca ggctgaaaat tctttttata ttgtcaggta tttcttaaat tatcttaatc
      181 cttaaacaag gaaataaatc agttccagat ttacaacgcc atcatggacg aaaaatgaag
      241 ctttcaatct cagcgacctc agcgacggtg cgcctcacct tcgcaagagg tcgcttcacg
      301 cgataaatct gaaacgaaac ctgacagcgc gccccgcttc tgacaaaata ggcgcatccc
      361 cttcgaccta cgtaacagat ggaatcctct ctctgatggc agcaaagatt attgacggta
      421 aaacgattgc gcagcaggtg cgctctgaag ttgctcaaaa agttcaggcg cgtattgcag
      481 ccggactgcg ggcaccagga ctggccgttg tgctggtggg tagtaaccct gcatcgcaaa
      541 tttatgtcgc aagcaaacgc aaggcttgtg aagaagtcgg gttcgtctcc cgctcttatg
      601 acctcccgga aaccaccagc gaagcggagc tgttggagct tatcgatgcg ctgaatgccg
      661 acaacaccat cgatggcatt ctggttcaac tgccgttacc ggcgggtatt gataacgtca
      721 aagtgctgga acgtattcat ccggacaaag acgtggacgg tttccatcct tataacgtcg
      781 gtcgtctgtg ccagcgcgcg ccgcgtctgc gtccctgcac cccgcgcggt atcgtcacgc
      841 tgcttgagcg ttacaacatt gataccttcg gcctcaacgc cgtggtgatt ggcgcatcga
      901 atatcgtcgg tcgtccaatg agcatggaac tgttgctcgc aggttgcacc actaccgtga
      961 ctcaccgttt cactaaaaat ctgcgtcacc acgtagaaaa tgccgatctg ttgatcgttg
     1021 ccgtcggcaa gccgggcttt attcctggtg actggatcaa agaaggcgca attgtgattg
     1081 atgtcggcat caaccgtctg gaaaatggca aagttgtggg cgacgtcgtg tttgaagacg
     1141 cggctaaacg cgcctcatac attacgcctg ttccgggcgg cgttggcccg atgacggttg
     1201 ccacgctgat tgaaaacacg ctacaggcgt gcgttgaata tcatgatcca caggatgagt
     1261 aacatggcga cattttcttt aggtaaacat ccgcacgttg agctgtgcga cttgctgaaa
     1321 ctggaaggct ggagcgaaag cggcgcgcag gcgaaaatcg cgattgccga aggccaggtg
     1381 aaagtcgacg gtgcggttga aacgcgcaaa cgctgcaaaa tcgtcgccgg tcagacagtg
     1441 agttttgcag gtcacagcgt acaggttgtt gcctgatccc tttcccggcc tgtatcaaca
     1501 ggccggttct cttttcagac ttttgccctc tacgccccgt tcatccatgc tgatagcatc
     1561 gacattttgc cgaagcgacc atcatgccaa ccgttattac gcacgccgcc gttcccctat
     1621 gcattggttt agggttgggt tcgaaagtca tccctccgcg tctgttattt gccggaatca
     1681 tcctggcgat gctgcccgac gccgacgtat tgtcgtttaa atttggcgtt gcttacggca
     1741 atgtttttgg tcatcgcgag tttacccatt cgctggtgtt tgcgtttgtt gtcccgctgt
     1801 tatgtgtgct gattggacgc cgatggttca gggctgggct gattcgctgc tggctgtttt
     1861 taaccgtctc gttgctatcg cacagcttgc tggattcggt aaccactggc ggtaagggcg
     1921 ttggctggct gtggccgtgg tcagatgaac gctttttcgc tccctggcag gtgattaaag
     1981 tcgcgccgtt tgcattgtct cgttacacca cgccgtacgg gcatcaggtg attatttctg
     2041 aattgatgtg ggtgtggttg ccggggatgg tgcttatggg aatgttgtgg tggcgcaggc
     2101 gataaccgga tgcgaaaact cgcatccggc aacagcgcaa ttacttacga cgccaggtgg
     2161 tcccttgcgg gccatcttcc agcacgatcc ccatctcgtt aagacggtca cgcgccgcat
     2221 ctgccgccgc ccagtctttc gctttacggg catccagacg ctgttgaatt aacgcttcaa
     2281 tctcagccac ttcgctgtcg tctgcctgcg caccgctttg caggaacgct tccggttctt
     2341 gctccagcag gcccaataca gcggaaagtt tacgcaggtg agatgccatt gcattcgccg
     2401 ctgccatatc ttctgctttc agacggttca cttcacgcgc catatcaaac agcacggaat
     2461 aggcttccgg ggtgttgaaa tcgtcgtcca tcgcttcaat aaagcgcgct tcaaacgctt
     2521 cgccaccggc aggcgcaacg gttttatctg tgccgcgcag cgcaatgtag aggcgctcca
     2581 gcgccgcacg cgcctgcttc aggttctctt cgctgtagtt cagttggctg cgatagtggc
     2641 ccgacatcag gaagtaacgc acggtttccg cgtcgtagta tttcagcaca tcgcgcacgg
     2701 taaagaagtt acccagcgat ttggacatct tctcgcggtc aaccatcacc atccccgagt
     2761 gcatccagta gttcacatac tggccatcat gggcacaggt ggactgcgcg atttcgttct
     2821 cgtggtgcgg gaacatcagg tctgaaccgc cgccgtggat atcaaagtgg ttacccagct
     2881 gcttgcagtt cattgccgaa cattcaatgt gccagcctgg acggcccgcg ccccacggag
     2941 acggccagct cggttcgccc tctttcgaca tcttccacag aacgaagtcc atcgggttgc
     3001 gtttgtcgtc gaccacgtca acgcgcgcac ctgcctgcag ctggtcgaga tcctgacgcg
     3061 acagcacgcc atatgtcgga tcggtcggga cgtcgaacat cacgtcgccg ttgtccgcca
     3121 cataagcgtg acctttggcg atcagttgtt cagtgagttc aataatttct gcgatatggt
     3181 gcgtcgcgcg cggctccata tccgggcgca gaatgttcaa agcatcaaaa tctttgtgca
     3241 tttcggcgat catgcgatcc accagcgcca caaagctttc gccattttca ttggcgcgtt
     3301 tgatgatttt gtcgtcgata tcggtaatgt tgcgcacata cttcagttta tagccgagga
     3361 aacgcagata gcgcgcaacc acgtcaaaag caacaaaggt acgcccgtga ccgatatgac
     3421 agagatcgta aacggtgatt ccacacacat acatgccgac ttccccggcg tgaataggct
     3481 taaattcctc tttttggcgt gtcagagtat tgaagatttt tagcatcgaa gattccgttt
     3541 agacatgtgt gggtaattga gttgcgtata atacccatat ttcccgcgcg aatcagcata
     3601 cattgcgtga tgatccgatc gtgcggttat gctataacac caccctatat atgacccgaa
     3661 ctgggttgaa gcaccaatca aacggaacag gatgcaaaaa tggttacttt ccacaccaat
     3721 cacggcgata ttgtcatcaa aacttttgac gataaagcac ctgaaacagt taaaaacttc
     3781 ctggactact gccgcgaagg tttttacaac aacaccattt tccaccgtgt tatcaacggc
     3841 tttatgattc agggcggcgg ttttgaaccg ggcatgaaac aaaaagccac caaagaaccg
     3901 atcaaaaacg aagccaacaa cggtctgaaa aatacccgtg gtacgctggc aatggcccgt
     3961 actcaggctc cgcactctgc aactgcacag ttcttcatca acgtggttga taacgacttc
     4021 ctgaacttct ctggcgaaag cctgcaaggt tggggctact gcgtgtttgc tgaagtggtt
     4081 gaaggcatgg acgtggtaga caaaatcaaa ggtgttgcaa ccggtcgtag cggtatgcac
     4141 caggacgtgc caaaagaaga cgttatcatt gaaagcgtga ccgttagcga gtaatcgtgg
     4201 cgacactctt tattgcagat cttcatctct gcgtggaaga accggcgatc accgccggtt
     4261 ttctgcgttt tttagcgggg gaagcccgca aggccgacgc gctgtatatt cttggcgatc
     4321 tgtttgaagc atggattggc gacgacgatc ccaacccact ccatcgccag atggcggcag
     4381 cgatcaaagc ggtgtccgat tccggcgttc cctgttattt cattcatggc aaccgtgatt
     4441 ttctgctcgg caaacgcttt gcccgtgaaa gcggcatgac attattgccg gaagaaaagg
     4501 tactcgaact ttatggtcgc cgggtgttga ttatgcatgg cgacacgctg tgcaccgatg
     4561 acgcgggtta tcaggctttt cgcgccaaag tccacaaacc ctggctgcaa atgctattcc
     4621 tcgccctgcc gttgtttgtg cgcaaacgca ttgccgcgcg aatgcgagcg aacagtaaag
     4681 aagccaacag cagtaaatcg ctggcgatca tggacgttaa ccaaaacgcg gtagtcagtg
     4741 cgatggaaaa acatcaggtg caatggctga tccacggaca tacccatcgc ccggcggtac
     4801 atgaacttat cgccaaccag caacctgctt ttcgcgtggt actcggtgcc tggcatacgg
     4861 aaggttcgat ggtgaaagtc acggcggatg acgttgagct gattcatttt ccgttttaaa
     4921 aaacccgcaa ctttgctgat ttcacagcca cgcaaccgtt ttccttgctc tctttccgtg
     4981 ctattctctg tgccctctaa agccgagagt tgtgcaccac aggagtttta agacgcatgt
     5041 cttcccgcaa taatccggcg cgtgtcgcca tcgtgatggg gtccaaaagc gactgggcta
     5101 ccatgcagtt cgccgccgaa atcttcgaaa tcctgaatgt cccgcaccac gttgaagtgg
     5161 tttctgctca ccgcaccccc gataagctgt tcagcttcgc cgaaagcgcc gaagagaacg
     5221 gttatcaggt gattattgcg ggcgcaggcg gcgcagcgca cctgcctggt atgattgccg
     5281 ccaaaacgct ggtgccagtg ctgggcgtgc cagtacagag cgccgcactg agcggtgtcg
     5341 atagcctcta ctccatcgta caaatgccgc gcggcattcc ggtgggtacg ctggcgattg
     5401 gtaaagctgg cgcggcaaac gcggcgttac tggcagcaca aattcttgcg actcatgata
     5461 aagaactgca ccagcgtctg aatgactggc gcaaagccca gaccgacgaa gtgctggaaa
     5521 acccggaccc gcgaggtgcg gcatgaaaca ggtttgcgtc ctcggtaacg ggcagttagg
     5581 ccgtatgctg cgtcaggcag gtgaaccgtt aggcattgct gtctggccgg tcgggctgga
     5641 cgctgaaccg gcggcggtgc cttttcaaca aagcgtgatt accgctgaga tcgaacgctg
     5701 gccggaaacc gcattaaccc gcgagctggc gcgtcatccg gcctttgtga accgcgatgt
     5761 gttcccgatt attgccgacc gtctgactca gaagcagctt ttcgataagc tccacctgcc
     5821 gaccgcaccg tggcagttac ttgccgatcg cagcgagtgg cctgcggtgt ttgagcgttt
     5881 aggtgaactg gcgattgtta agcgtcgcac tggtggctat gacggtcgcg gtcaatggcg
     5941 tttacgtgcc aatgaaaccg aacagttacc ggcagagtgt tacggcgaat gtattgtcga
     6001 gcagggcatt aacttctctg gtgaagtgtc gctggttggc gcacgcggat ttgatggcag
     6061 caccgtgttt tatccgctga cgcataacct gcatcaggac ggtattttgc gcaccagcgt
     6121 cgcttttccg caggccaatg cgcagcagca agcgcaagcc gaagagatgc tgtcggcgat
     6181 tatgcaggag ctgggctatg tgggcgtgat ggcgatggag tgttttgtca ccccgcaagg
     6241 tctgctgatc aacgaactgg caccgcgtgt gcataacagc ggtcactgga cacaaaacgg
     6301 tgccagcatc agccagtttg agctgcatct gcgggcgatt accgatctgc cgttaccgca
     6361 accggtagtg aatagtccgt cggtgatgat caacctgatt ggtagcgatg tgaattatga
     6421 ctggctgaaa ctgccgctgg tgcatctgca ctggtacgac aaagaagtcc gtccggggcg
     6481 taaagtgggg catctgaatt tgaccgacag cgacacatcg cgtctgaccg cgacgctgga
     6541 agccttgatc ccgcggctgc cgccggagta tgccagcggc gtgatgtggg cgcagagtaa
     6601 gttcagttaa gcaggtgctc atttgattgc cggatgcggc gtaaacgcct tatccggctt
     6661 accgctccgg tacccattgt aggcctgata agacgcgtca agcgtcgcat caggcatggt
     6721 gcaccgattg ccggatgctc tcatcactac agcgaaatac aggtccccgc ttcgcccgcc
     6781 agcgtctctt caattcgcga taacgcccca atccacgcgg gtttaccacg gcttctgaca
     6841 taaccactta ccgccgttac cttcggcccc atcgaaccat cggctttggc aaatggcgct
     6901 aactcatccg gtgtggcatg gcgaatggcg cgttgctgcg gcgttcccca gttttcataa
     6961 accgcgtcag catcggtgag gatcaccagt ccatcggcat taatctgctc ggcgagcaac
     7021 gcagcggcga gatctttatc aatcaccgct tcactccctg ccccatcttc cgtcacaggc
     7081 acaccgccgc cgccactgca aatcaccaca tgcccctctt tgagcaacaa ctcgatggct
     7141 tcgctgtcga gaattttacg cggttgcgga gacgccacca cccggcgcaa atatttacca
     7201 tcacgtttca tctgccagcc gtaagccgct tccagtgcct tttgttcttc tggctgataa
     7261 accggaccaa taaatttctc tggctgcaaa aacgccggat catcaggcga aacctcaatg
     7321 cgcgtcagca ccgtcgtgac aggcggcatc tgcggctgtg cgctcaaact ctgcgccagc
     7381 atatagccaa tcatcccctg gctttccgcc accagcacat ccagcggata cggatctacc
     7441 tctttccacg ccagattctg caatgccagc agccccacct gcggcccatt gccgtgaaca
     7501 atcgccagcc gataagaacg ggccaggcgc gccagcgcgg gtacagcact ggcgatattg
     7561 cgatattgat tttctgccgt cagcgcctca ccgcgctgga gtaaggcgtt gcccccaaga
     7621 gctacaacca gtgttttcat ggttttcctt gtaataattg ttggccaagc cagaacccca
     7681 gcagcgtatc tgcccctgac gtatgcccca gcgccagcag cgaatcaatc gcaacagcgg
     7741 tacgcttcgg gcaactcaga gcatggacaa agtgcaggag tggcgaggcg aaatatcctt
     7801 gcgcggcata acgtaaataa ctgacgctca ccgctgtggt aacgagttga agattgtcgg
     7861 aacaggcaaa aaacgggcga ccggcgcgcg catctaaagc gccataatac caggccgcca
     7921 gcagcattcc gctcaacgtg tcatcatgac ttggcgttaa tccggggcct ttacccagcc
     7981 agtgccgcca gtcggtctta acgccattga gcgcggcctg aaaacagtga cgaaactggc
     8041 gtaattcagc gggcagcgga tcgcttgccg ccatcgccag tggtccgaaa agtccggttt
     8101 cctccgcgcg ttgcatccat gcagctgcaa gcggttgagg atgcgaaggc ggcgtaatac
     8161 gcagcaaaca ataacgctgt ggctgtttaa ccgtgaaacg cccgaggcga atcccttgag
     8221 ccacaacctg tggtcgttca ttgccgcata atccaccgca taacgcatcg aattgcgcgc
     8281 ggcgaagcac ccatcctccg gggccgaaac cactaccctg acgatgcagc gttaacagtt
     8341 cgccgctttc cgtcatcagg ttaatcgccc gtcgccacac tcccgctaac cgccaggact
     8401 ggcgataatt cggtgcacta cggctggcaa gcagaggatg gatgatcgtc attgcgcgtt
     8461 cctcaaccaa tccccatgct ttcagccagt gcttccagcg cctgttcaaa acacgccagc
     8521 ggtgcccgca cagtgcctgc gccaatctgc ccgatccccg cctctttatg ggcgataccg
     8581 gtattgatga gtggcgtaat gccggtttct accacgcgac gaatatccag ccccaggcac
     8641 gcgccctgga aatcccagcc ggggatctgc aactgcatat tgcgctcaag gtaaatttcc
     8701 gccatctctt cagataccgc tctggccgct tccatgccac ccgcaccgac aaagcgcgtt
     8761 acgccaggcg ctgcgatcat tgccgcacct ccgataccaa aggtttcggt aatcgcgcta
     8821 tcgcccatat ccgggttcgc ctgctcctgc gagaagccgg tgaaaaacag accttgcgga
     8881 gtgtttacag gcgcagtaaa ccagcgttcg cccagcccgc ttacccgaat cccgaacata
     8941 ttgccgttgc gggtcattgc cgtgacgatg ctgcctgcgc ggatcatcgc gccagcatcc
     9001 atcgccgcct tgcagtaagc catcgcgagg ttgaggaaga actgatcggt cacgctgagg
     9061 aaatccatca cttcggcgat gtgctgttta tcatgatcga ggcgagcaat ttgtggggcc
     9121 agcgcacgca ttaacagtgc agaggaagca atattgcgtt gatggaactc atcgcccatc
     9181 gtaatgccct gcgccatcat cgccgtgaga tcgataccgc gctccatgcg ccccagcgcc
     9241 gcgcttaata ctggcattaa cacatcgcgc atccagcgat ggcgagtcag gacatcttcg
     9301 ccgtaagcgc caaaacgcat cactttgccg ataccttcgt tgaggttgca gtacgcccgg
     9361 ttgccgtcgg tcacgttctc gaccaccagc atcggcatac tggcagaagt aataccgccc
     9421 attggaccga cggcattcac atggtgacaa ggaatgaagt tcacttcccc ctgctccagt
     9481 attgccagcg cctgcgcttc atctttcgcc cagccttcga acagacatgc gcccacgcac
     9541 gcccctttca tgggtccggt catctcctgc cagcgcattg gcggcccggc gtgaagcagt
     9601 gttttgccct cgtttagttc gctgataagt gaagaagccg gttgcacatc cagccagtgt
     9661 ggacgagcac gacgaatttg ttcgataacc gcagcattgg cttgcgccac tgatgtaaac
     9721 atgggaaccc cttattgcaa acgttctaat aaacgagcca gttttttatt gccgccggcg
     9781 actggcgacc attggtaatg cacaaccggt ttgctggcgc tttgtagctc cagcgcaaag
     9841 ctgcgtaatc cgatgttaat cacggcgacg ttttccagta atgacggtgt gtgttgctgt
     9901 gtggcagacg agagcggatg aattaacgct gccgccagca aggtggcttc cggtagcgaa
     9961 ctcactaccg cgatccccgc atcttccagc gtggcgattt gctgcgagcg gcattgcggg
    10021 tcacgttcgg tacctgtcac cgtggcaatg gcgtacagcg gttggctgtc tgaacgcgcg
    10081 gcacaggctt tttgccaggc gctcaccagc gaggcagcag gatcggcggt cgcaccgaag
    10141 ccaatcacga catcaagcag caacacgcgc acttgcggtt tagcgccgag atcggcaatt
    10201 aactggttgc gtaaggctgg gtcgatcatc ggatggggac gcccgacggt gtagaaatca
    10261 tcgcccagat caatgatctg gtgaccatcg gcgtccagca tcatgccgtg atgatgggta
    10321 tcgtcggctt ccacgccaag gtgcccggca agtaatcccg ccgcttcggc agcaagcgta
    10381 ccgccggtat acaaaccgca aataaatccg ctgctgacag gcgctattgc gttacgtcgc
    10441 gccgtgacgc gtgaaagcag gcaagccagg cgtgcggcct catccagcga ggaggcaaac
    10501 cagacattct cgtcgcgggc caccgccggg gtataaccta aaaacagcgc caccgtcggt
    10561 ttgccggttg ctttcatggc attaacaatt ttcagacgca cagcttcggc aggtggtttt
    10621 gaaacaaatg ccagcacttc gcttttctcg tctgcactga gcatttccag cgctgttagc
    10681 gcactgatgc cgcccacttc acggctgagg tcgcgcccgc caaggccaat cgcgtgagta
    10741 attccctccc ctgccagcgc aatctgcgaa cacagctcct gaatcccggt accggaagcg
    10801 ccaatgacgc caatattgcc ttctggcatc acgttagcaa aagccagcgg tgtgccggca
    10861 atcatcgacg taccgcagtc cggccccatc accagcaagc ctttttcccg cgcgcgggtt
    10921 ttaagttgga tttcatcttc cagcgtgacg ttatcggaga acatcatcac gttgaggttg
    10981 cgatccagcg cctggtttgt cagctccgcc gcatactcgc cagccactga aatcagcgcc
    11041 aaattggcat cgggtaattt ctgacaggcg ctgtcccagc gacgcacctg cgtcaacgcc
    11101 tggctgctgc ctgacccctg cgccagttgt tttagcgcct cttcaagctg ctgcataatc
    11161 gcctgcgcga tccccgcatc cgccgcttcg ctacgaattg ccacgcaaat atcgttcggc
    11221 gtggcgttat taaaatcgtc atgccagaaa cctgtggtat ctaataacgc tttattggcg
    11281 ggcgtaccca tcattacgga aacatcatca acattttctg attcgctgag ttttcgtgaa
    11341 ataatcatta aactgaccga atcctgaaaa cacccttttt taataaaggc gtggatcata
    11401 acaactcctt aaaaattgca cccatggcaa ttgcacgagc agtgttaatg tggcgattac
    11461 ggtatcgatg acaacgagta aataaggtga tgaatatcac acagggatta ttaacaaaag
    11521 aattcaaata accgccaggc tattacgcat tattttttaa taagcgtggt tgttttaaaa
    11581 atcaggttaa aagcgcgaag attatcaaat aaattctaac tatatttttt tgcctgtctg
    11641 gatcacataa tccagatatt ttccctgtat gttaattgca gtcatgcttc acaccgtcgt
    11701 taaaaaggaa gacagatgaa aatcagtcgg gaaacactcc accagctaat tgagaataaa
    11761 ctctgccagg ctgggttaaa acgtgagcac gctgcaaccg tggctgaagt attggtttac
    11821 gccgatgcca gagggatcca ctctcatggc gcggtgcgcg tggaatacta cgcggaacgc
    11881 atttcaaaag gcggcaccaa ccgcgaaccg gagtttcgtc ttgaggaaac cgggccgtgc
    11941 tcggcaattt tacatgccga caatgccgcc ggacaggtcg cggcgaaaat gggtatggaa
    12001 catgccatca aaaccgccca gcaaaatggc gttgcggtgg tcggtatcag ccggatgggt
    12061 cacagcggcg caatctctta ttttgtgcag caggcagccc gcgccggatt aattggtatt
    12121 tcgatgtgcc agtccgatcc aatggtggtg ccgtttggcg gcgcggaaat ttactacggt
    12181 actaacccac tggcctttgc cgcgccggga gaaggcgacg agatccttac ctttgatatg
    12241 gcgactaccg tacaggcatg gggaaaagtg ctcgacgccc gctcgcgtaa tatgtctatc
    12301 ccggatacct gggcggtcga taaaaacggt gcaccaacaa ccgatccgtt cgcggtacat
    12361 gctctgctcc ccgccgccgg gccaaaaggg tatggcctga tgatgatgat tgacgtcctc
    12421 tcaggcgtct tactcggctt accgttcggg cgacaggtta gttcgatgta tgacgattta
    12481 cacgccgggc gtaatttggg gcaattacat atcgttatta acccgaactt tttcttctcc
    12541 agcaaattat ttcgtcaaca tcttagccag accatgcgcg aattaaatgc cattaccccc
    12601 gcgcccggtt ttaatcaggt ttattatccc ggacaggatc aggatattaa acaacgcaaa
    12661 gccgccgtcg aaggcatcga aattgttgat gatatttacc agtatttaat ttccgacgcg
    12721 ctttataaca cgtcatacga aacgaaaaat ccctttgcgc aataattatt gagacaggac
    12781 tttcttatga ttacacattt tcgtcaagcc atagaagaaa cgctgccctg gctttcctct
    12841 tttggcgctg acccaacggg tggtatgacc cgtttacttt attcgccgga atggctggaa
    12901 actcagcagc aatttaaaaa aagaatggca gcaagcgggc tggaaacacg tttcgatgaa
    12961 gtggggaatt tatatggtcg cctgagtggc accgaatatc cacaagaagt ggttctgagc
    13021 ggttcgcata tcgataccgt ggttaacggc ggtaacctcg acgggcaatt cggcgcgctg
    13081 gcggcgtggc tggcaattga ctggctgaaa acgcaatacg gcgcgccgct acgtacggtc
    13141 gaagtggtgg cgatggcaga agaagaaggc agccgcttcc cgtatgtctt ctggggcagt
    13201 aaaaatatct ttgggctggc gaatcctgac gacgtgcgga atatctgtga tgccaaagga
    13261 aatagttttg tcgatgcgat gaaggcttgc ggatttactc ttccgaacgc cccactaact
    13321 ccgcgtcagg atattaaagc ctttgttgaa ctgcatattg aacagggctg tgtgctggaa
    13381 agtaatgggc aatcaattgg cgtggtgaat gcaattgtcg ggcagcgtcg ttatacggta
    13441 acgctgaacg gcgaatcaaa ccatgcaggc accacgccga tgggttatcg tcgtgataca
    13501 gtttacgctt tcagtcgcat ttgccatcag tcggtcgaaa aagcgaaaag gatgggcgat
    13561 ccgctggttc tgacctttgg caaagtagag ccgcgcccga atacggtaaa tgtggtgccg
    13621 ggtaaaacca cgttcaccat tgattgtcgt cataccgacg ctgccgtgct gcgcgatttc
    13681 acccaacagt tagaaaacga catgcgggcg atttgcgatg aaatggacat tggtattgat
    13741 atcgatttat ggatggacga agaacccgtg ccgatgaata aggagctggt cgccaccctg
    13801 acagaattgt gtgaaagaga aaaactgaat taccgggtga tgcacagtgg tgccgggcac
    13861 gacgcgcaaa ttttcgcgcc tcgcgtacca acctgcatga ttttcattcc cagcatcaac
    13921 gggatcagcc ataacccggc ggaacgcacc aatattaccg accttgccga aggggtcaaa
    13981 acgttggcac tcatgcttta tcaacttgcc tggcagaaat aaggagtcat aaatgggata
    14041 tttaaataac gtcaccggtt accgcgatga tttactggct aaccgtgcga ttgttaaaca
    14101 cggtaatttc gcactgttaa ccccggatgg tctggtaaaa aatattattc cgggctttga
    14161 aaattgtgac gcgacaatcc tctccacgcc aaagctgggt gcctcttttg ttgattatct
    14221 ggtcacactg catcaaaacg gtggcaacca acagggcttc ggtggcgaag gcattgaaac
    14281 gttcctgtat gtgatctctg gaaatatcac tgccaaagcc gaaggcaaaa catttgcctt
    14341 aagcgaaggt ggctatcttt attgcccacc aggctcctta atgacgtttg ttaacgccca
    14401 ggccgaagac agccaaatct ttttatataa gcgccgctat gttccggtag aaggccatgc
    14461 accgtggctg gtttctggta atgccagcga actggaacgc attcactatg aaggtatgga
    14521 tgatgtgatc ctgctggatt tcctgcccaa agagttaggt tttgatatga acatgcatat
    14581 cctctctttt gcgccaggtg ccagccacgg ttatatcgaa acacacgttc aggaacatgg
    14641 tgcctatatt ctttccggtc agggggttta taacctcgac aataactgga tcccggtgaa
    14701 aaaaggcgat tacatcttta tgggcgctta ttctttacag gctggttatg gcgtagggcg
    14761 tggtgaagcg ttcagctata tttactcgaa agattgcaac cgcgacgtag agatttaagt
    14821 cataccactc gcctgattat ttttagctat gttggatttt gccacggttt ataccgtggc
    14881 tttttttgta gtaaattcag gtaaaaaaag atgcgaaata aatcgcatcc gctttaaagg
    14941 taagggtcag tttttaattc cctgacctat tttaatggcg caggcaatat ttcgcgcgct
    15001 gttgaagaga ttggtttcac cgctggcgag cacttcggct aaaggtgcca gacgaggcaa
    15061 aatgctgaat accgcgtcaa tgccgtactg gtgcaccact tccacgccat cacccaatac
    15121 gccagcaatc ccaatcaccg ggacattaaa ctgcttcgcc accgacgcaa cgcccagcgg
    15181 cgctttaccg cctgccgttt gcgagtcaat gcgcccttcc ccggtgatca ccagtgctgc
    15241 gccctgcacc gcctgctcca ggttgaccgc ccgcagtaca atctcaatac ccggtttaat
    15301 atccgcattg aggaataccg ccgccgcaat acccataccg cccgcagcgc cgccgcccgc
    15361 catctggcag acattaattt cagtttgctg ttgtaaaaca cgggcgtaat tttgcagccc
    15421 ctgttcaagt tcttcgacca tctccggcgt tgcccctttt tgcgggccaa acaccgccgc
    15481 cgcgccgcgt gcccctacca gcgggttatc aacgtcacat gctacttcaa tatggcaatt
    15541 cgccagacgc ggatcgcatt catccatctc aatgcttgcc acgcgcgcta aattaccgcc
    15601 gttagccgcc agcacctggc cgtcggcatc aaggaaacgc acaccaagcg cctgagccat
    15661 gcccataccg ccgtcgaccg tcgcactgcc gccaatgccg agaataatat ggcgaatgcc
    15721 gttatccagc gcatgacgaa ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt
    15781 acgcttttca ggcgcgacca gcatcaggcc acttgctgcc gccatctcaa tcaccgccgt
    15841 tttcccgtcg ccggtaaggc cataaaaagc attcactgtt tcgcccatcg gcccgcagac
    15901 ttcaagcgtc acgatgttgc cgcccgtcgc ggcgaccatc gcatccaccg tcccttcgcc
    15961 gccatccgct atcggcaaac agatatagtt cgcatcggga aagagggtcg aaaacccggc
    16021 tttaattgcc tgacaacatt tttctgcact taagctctct ttaaaagagt ctggcgcaat
    16081 gacaatcttc attatcaatc ctttttcaac aaattattcg atgcgttccc acggaataag
    16141 attatccata agaacagcca gtaaaattcc gaccagtaag ccgttactga gcagaggacg
    16201 cagcgttaat ggaaggtctt gcagatacac aggcggtaat gccattaaaa atatgccgac
    16261 aaataacggc aatgcgagtc gataaatatt gcgagcggta aaggttattt gctggctaaa
    16321 caccagcgcg gaaaagagta aaggcagata agaaaccagc atgaccgcgc tactcacggg
    16381 taaagggata ctgcaaaaca gtcgtgtgag tgccggaacc agcgccacca gcaggcaaat
    16441 aacgctgcca taaataaacg aacgccgcgt gtaatcgcct gtctgggtta ataaaccaat
    16501 ggatgaaaca aacggtgaaa atggaattac cgccagcggt acggttatca gcgtcataaa
    16561 tccggtcgcc acaaagctgc gacgataacg cgtattcccc gcgccctgct gcggataaaa
    16621 aacatccgtg ccccgaatcg caccgtaggt attgctgata ttcaccagac ctgtaatcac
    16681 cgccgtcaga ataattcccg gagacaaagc acctccactg ccgagcggga accactgcca
    16741 gtgcaactca ccggagagcg agtgcgaaga aggaaagcaa aagtaccaca acaaccagcc
    16801 ggttatggtg ccgaccagca ggccataacg ggcaaaacgt tgcggcagga agataatcat
    16861 ccccagtacc aggcacatca ccgccaccga gagcgcgaac ggcggtaact gaattttaaa
    16921 atttgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag
    16981 ctgcgcgccc agcatcaaca taaacaagac catcaccgac ggcgtaaata accgtgccag
    17041 gcgatggcct aatccgctaa aaccaatcaa catcgtcagc acgccggaga gcgcaatccc
    17101 caccgccagg ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt
    17161 gatagtaagg atggttcccc accacaggcc acccggccct tccataatag cgcgacgatg
    17221 accgcagaac gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag
    17281 cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac
    17341 cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctcac
    17401 tgcaaaattg aacataaaca caccttaacc ggagaatgcc cgccgcaagg acgggcattg
    17461 caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag
    17521 ccctgttcaa tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca
    17581 atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga
    17641 acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg
    17701 cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt gccgaacatt
    17761 ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat acagttttgc
    17821 agaccggcaa taccgcccca tgcttccatg atgttgccgg ctttcatttc cggagggcat
    17881 ggtgagtggt cggaaaccag gcagtcgatt tcaccgttaa acagtttttc ccacatgcct
    17941 ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtgcc gatttcttcg
    18001 aactgatcgg tatccagtac aaagtaatgc gggcaggatt cacaagtaac gtcctgacct
    18061 tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc
    18121 agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta
    18181 aataccggac gcgaagccac atagtcatgg gcagttacgc gaccttcacg tttcgcttct
    18241 tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt
    18301 tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg
    18361 tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca
    18421 tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg
    18481 atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt
    18541 gcaggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg
    18601 cgagtaccgg tttcataacc ttcccagtgg ctacgacccg gttcagaaat atgggtgtgc
    18661 gcatcaacca tgcccggcga aaccaccaga ccagacgcat ccataacttc ttttgcatcg
    18721 cccagatcct gaccgatagc agcaattttc ccgcctttaa cggcgatatc tacaacgcga
    18781 gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac
    18841 tccttgtaaa atacagattg gtttttaact caatgttcaa tatcgggatt aattaaccta
    18901 tggttttttg ctctcctgtt ttttctgctg ttgtacgttt ctttaataag gcgtaggccg
    18961 caaaggcgac gatgacgccg acaaaccatg aaacacgcga taacggttcc ataaagtgaa
    19021 taaacttacc gccaagagat aaaataacgg ccaccagagt tactgaaaac gcagtgaggt
    19081 taaaaccgtt atcgtaatat ttataatcgc caggtgcggt atacagttca tcaagattaa
    19141 tttgtccgcg catcaccaca aaataatgcg ccatcatgac accaattacc ggaccaagca
    19201 ttccgccgat aatatcgagg aaaagataaa tgctgtcctg attttccatt aatttccacg
    19261 ggcagatcag caagctgata atactggcaa tcagtacgcc gtttttatag gtcagttttg
    19321 tcggtgcaat ggcggcaatc tgataaccgg ctggaataat attaccggtc gcgttagtgg
    19381 agatagttgt catcagaata accagtaccg caaagaacga ggcgaacagg ctgtcccaac
    19441 gctgaacaat atccagcacg ttccaggtat cagcgccgta gtgaatactg gctccggcaa
    19501 taatacatac cccggcgacc gcaaacagaa tataggccac aactaaaccc agcgtttgcc
    19561 ccagcgcctg ctcacgaaac gagtgggcgt tttgcgtaaa gtcggatgcg ctcaccgccg
    19621 gtgccgccca gaccgcaact accgcgttaa tcaccaccag gaacaggaag ccaccgtttt
    19681 ctgctttctg aataccgctc ggaatgtagt caaagattgg accgatcccg accagtgaaa
    19741 tcgcccaaat cgccataccg ccgaaaacga tatagatgca cgggttaaga atggcagtga
    19801 atttatttaa aactttgcca ccgccaaaac ctataccaac gttgaccagc cagaagatta
    19861 agaaagtaat taagcccggt agagaaaggc ctaacagagt gaaatcacca ccgagagtta
    19921 aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc cagtgacccc gcgtaacatt
    19981 gcaaaccaaa ccacatgatg gcggcaattc cgccccttaa taatccggga aacagtgcac
    20041 cacgtacacc gtaagaagca cgcaggatca tggcaaaagg cacaccgtat ttactgcccg
    20101 cagcaccgtt taataccatt accgcggcaa tgaaaaaggc gctgaggata attgccagca
    20161 taatactaaa ggtagacaag ccgagaataa aaaagccgcc gaccatcaca taattgggaa
    20221 cgttatgaac cgaacccatc cataaggtaa aatagttaaa tgttttccag gtccgctggc
    20281 tttgcgtttt cggcaataga tcttcgctat agccgcgttg ctggaatagt tttctctgat
    20341 gttccataaa tccctcttaa tttcacgtat cgaataacgt tgttttaatc tagctatgag
    20401 gaacaaataa aataaaaata ttatttttaa tactgaagca acatctttgg ctgctttctt
    20461 ttttaatatt gctggccgat tcattttctg atatcggcca tttttattgc gggtatcagg
    20521 ccagtttatg gttagccatt aattccagcg cctgcactaa cgcagagtga tccaactggc
    20581 tgccaccgtt tgccgcgcag gtattaaata actcctggca ggtcgcagtg tttggcaggt
    20641 tcagcgctag cgctttcgca ctttgcagtg ccaggttgag atctttctgg tgcagcgcaa
    20701 ttttgaaacc tggattaaaa gtgcgtttaa tcatgcgctc accgtgaact tccagaatac
    20761 gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg taccgggtcc gcaccggctt
    20821 ttgaagcaaa tagcagggct tcagaaaccg cttcaatatt gagcgccaca ataatctgat
    20881 ttgccacttt gcaggtttga ccatcgccgt taccgcccac gagggtgata tttttaccga
    20941 gcagttcaaa cagcggttta acacgttcaa ataccgcttc atcaccgcca accataatcg
    21001 acaacgtccc ttcacgcgca ccgatttcac cgccggagac tggcgcatcg agataatcgc
    21061 cgcccagttc attcacctga cgagcgaaac gcttagtttc aatcggggaa atggagctca
    21121 tatcaacaat ggttttgccc ttcagcgagg ctttggtaca accattttca ccgaacagaa
    21181 cttcttcaac ctgtggtgta tctggcacca taataaaaat gatgtccgat gcttccgtta
    21241 cctggcgcgc agtttcaaca ctgacggcac ccagtgacag taattcatca gcaaccggac
    21301 caatggttgt gacatgcagt tgatgaccgg cacgcgccag attaatggcc atcggtgtac
    21361 ccataatgcc taagccaata aatcccagtt tcataattaa cctcttttaa atttcgcttt
    21421 tcctgaattc agacaacacg atcccctgcc aacttagcgg cctaaaaaag cattgcctga
    21481 acagcgttac gttttaacgg tacggatcca tccagcgtaa accggcttcc gtggtggttt
    21541 ggggtgtata ttcacaccca acccaaccgt tgtagtcaga attttcgatt accttaaaga
    21601 gataatcata attaatttct ccggtccccg gttcgccgcg atgcggatta tcagcaattt
    21661 gcaggtgacc aattttatcg gcccactgag tcatggtgtt ggttaattca ccttccatcc
    21721 gctgcatgtg ataaatgtca tactgaattt tcaaattgca gcaaccaaca tcatcaatca
    21781 atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg aatatcaaaa tggttaatag
    21841 gttcaatcag taataaaata tcttctttca tcagcatatt cgcggcataa cgcaggtttt
    21901 ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc cggcgtttta ccgaccagac
    21961 agttaatttt tttattaccc agcgcacggg cataacgaat cgctgctgct acgccatccc
    22021 gaaactcttc ttcacggcca ggaatacagg caataccgcg ctcccccgcc gcccagtcac
    22081 ctgccggtaa attgtgcagc gtatgttcga gtttattact cgccagcacc tgttttaatt
    22141 cttcaatgtc gtagtcataa ggaaacataa attcaacgcc gcgaaaacca cactgcgcag
    22201 ctttctcaaa acgggcgaga aaatcatatt caccaaataa catcgataaa ttagcagaga
    22261 aacgtaacat tatttatctc ccttattcat agtgcatgaa gcaggtttca gtcggtgcgt
    22321 ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt atccagttcg ctgcccatcg
    22381 aaatattggt cacacgctca agaataactt ccacgactac cggtacccga tattgcgcca
    22441 ttaaggcttt cgcctgttca aaggctggcg caatatcttc cggtttgaag acccgaatag
    22501 ctttacaacc taaaccttcc gctactttta cgtggtcgac gccgtaaccg ttcacttcac
    22561 tggagttaat attttcgaac gcgagctgta cgcagtaatc catgtcaaag gcgcgttgtg
    22621 actgacgaat cagccccaga taagcgttat tgaccagtac atggatgtac ggaatgttga
    22681 actgtgcgcc aacggctaat tcttcaatca ggaactggaa gtcgaagtca ccggaaatcg
    22741 ccaccacatt ccgttctgga tcagcagcac acacgcccag cgccgccgga atggtccagc
    22801 ccagcgggcc tgcctgacca cagttgatcc agtgacggtc tttaaagaca tgcagcattt
    22861 gtgccgcagc gatttgtgac aggccaatgg tggtgacata acaaacatcg cgaccaaagg
    22921 ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg cacgttgtcg aagtgggttt
    22981 tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac ccattcttta cgacacggca
    23041 gacgacccgc tttttgcatc tcctgcgcca cttcaaccag cagtgtcagc gccgctttag
    23101 catcagagac aatgccgaga tccggacaca gcacgcgacc aatttgcgtc ggctcaatat
    23161 cgatatgaac gattttgcgc ccttcggtgt atttctctac cgaaccggta tgacggttag
    23221 caaaacggtt accgatacca aacaccatgt cggacgccag cagcgttgcg ttaccgtaac
    23281 gatgcgcggt ttgcagaccc accatcccgg ccatcagttc atgatcgtcc gggatacagc
    23341 cccagcccat cagcgttggg atcaccggaa cgctggtcag ttcagcaaac tgttgtaaca
    23401 gtgcagctgc gtcagcatta attaccccgc ccccggcaac aatcaccgga cgttcggcct
    23461 ggattaacat ttctacagct ttttcgatct gcatacggct ggcagcaggt ttgtagaccg
    23521 gcagcggttc gtacatatca ggatcaaact cgatttccgc aacctgaacg tcgaacggta
    23581 aatccaccag taccggaccc ggacgaccag aacgcatcag gtgaaatgcc tgttgcaaca
    23641 cacgaggcac cagcgccgct tcacgtactg taaccgccat tttacttacc ggcttggcga
    23701 tagcttcgat atcaacagcc tggaaatctt ctttatgcag acgggcgcgc ggagcctggc
    23761 cagtaataca cagaatagga atggaatcag cagaagcaga atagagcgcg gtgatcatgt
    23821 ccgtgcccgc aggaccggaa gtccccagac atacgccgat attccctgcc gttgcgcggg
    23881 tataaccttc cgccatgtgc gaagcacctt ccacatgacg cgccagaatg tgacgaatac
    23941 cgccgtgctt acgcatcgct gagtagaacg gattgattgc agctcccgga acgccgaagg
    24001 cggtggtgat accttctttc tccagcacat acattgccgc gtcaacggct ctcatttttg
    24061 ccattttatt cctacctcta ttaattggaa aaattttcca actttcaaat tttgtcaaaa
    24121 aaacccgctc cgcagagcgg gatgcgacag acactatgga tgtgctttca gtcccaacgc
    24181 cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc tgactgacaa aacgatcttc
    24241 tgtcagtctt gatgaaggcc cggagatgga gatagcggca acaacgctac caacatcatc
    24301 gtaaattgct gaagctatgc aattcagacc tacaacatgc tcttctttat ctacggtata
    24361 gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg ggcatatcca caagcgtagt
    24421 tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc atcaactcct cttccgccag
    24481 cggataaagc aacgccttgc ccgcaccgga agcatgcagc ggcagacgac tgcccagtgg
    24541 cgcacacatc ctgaccatcg atttacactc taactgacca attaataccg cttcattgcc
    24601 gttacggatc gcgacattga ccgtttcgcc ggaaagtaac atcaggcggc gcataaacgg
    24661 cccggcgacg gagaggacat cgcggttatg gatgtacgcc gcaccgacgt taaagacacc
    24721 taatcctata tgccaccagc ctaactgact gtcctgatag acaaaatccg ctgcctgtaa
    24781 aaccttcagc aagcgaaagg tcgtagagag cggcaaatcc agattgagag aaatatcgct
    24841 aaccgacgaa cttcccccac ttttttccag atattgcaga atcgcaattc cccgctctaa
    24901 cgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct ggcctgccgc gccgtctaac
    24961 ttccgtcata cctacgttaa ctccgaccta caagtgagca taaacaatga acaattagtt
    25021 tatcttatgc aagtcggttg caggcgtcac gcaaaacaga gttcctgttc aggaatactt
    25081 tcaacatcac agttgtcact gccgccgcga tcgatggtca gaaaatcggt gacgcgctgc
    25141 caggcgaaaa gtgggtgatg ccagacgtta cggtggtaat tcactccctg ttcgccgttg
    25201 gtaataaacg cccgcagggt tgacaggtct ggtttgtcgt cacctaacgc cacgaccacc
    25261 acaaacacct cacctttcat cgggataaag gcctgagtac ccagcggatg acgttcgagt
    25321 tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt taatgctgat aagcgtacgg
    25381 tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac gctccaccag gccattgtta
    25441 atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc cataagcact aaaggcttcc
    25501 tgacttaacg gtaatacctg aagtttcatc tttttgtcct tgtaacataa ttccgttcaa
    25561 ccaccgccga ccggtcagct tggtttcaat aacaggcaat ctaaaacctt aagagaacaa
    25621 cattaataca aaagttggaa tatttt
//
LOCUS       DAZGTR010000066        24451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGTR010000066 DAZGTR010000000
VERSION     DAZGTR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.066"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..363
                     /locus_tag="ACHLCY_002895"
     CDS             <1..363
                     /locus_tag="ACHLCY_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFP0370113.1"
                     /translation="YQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGY
                     TLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYL
                     SDHPKLQGIAQQNSFKHT"
     gene            519..2054
                     /gene="gadC"
                     /locus_tag="ACHLCY_002896"
     CDS             519..2054
                     /gene="gadC"
                     /locus_tag="ACHLCY_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP0370114.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2185..3504
                     /locus_tag="ACHLCY_002897"
     CDS             2185..3504
                     /locus_tag="ACHLCY_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP0370115.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLPQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVQELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3881..5246
                     /gene="dosC"
                     /locus_tag="ACHLCY_002898"
                     /pseudo
     CDS             3881..5246
                     /gene="dosC"
                     /locus_tag="ACHLCY_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            5271..7670
                     /gene="dosP"
                     /locus_tag="ACHLCY_002899"
                     /gene_synonym="pdeO"
     CDS             5271..7670
                     /gene="dosP"
                     /locus_tag="ACHLCY_002899"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP0370116.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRKNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVEIKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7928..8509
                     /gene="ddpX"
                     /locus_tag="ACHLCY_002900"
     CDS             7928..8509
                     /gene="ddpX"
                     /locus_tag="ACHLCY_002900"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP0370117.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYNAYRPQQAQAMLWQACPDPQYVVDVTVSSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8523..10073
                     /locus_tag="ACHLCY_002901"
     CDS             8523..10073
                     /locus_tag="ACHLCY_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0370118.1"
                     /translation="MKRSISFRPTLLALVLATNFPVSHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10075..11097
                     /locus_tag="ACHLCY_002902"
     CDS             10075..11097
                     /locus_tag="ACHLCY_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0370119.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11094..11990
                     /gene="ddpC"
                     /locus_tag="ACHLCY_002903"
     CDS             11094..11990
                     /gene="ddpC"
                     /locus_tag="ACHLCY_002903"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP0370120.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11987..12973
                     /locus_tag="ACHLCY_002904"
     CDS             11987..12973
                     /locus_tag="ACHLCY_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0370121.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12966..13892
                     /locus_tag="ACHLCY_002905"
     CDS             12966..13892
                     /locus_tag="ACHLCY_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP0370122.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(13948..14379)
                     /gene="osmC"
                     /locus_tag="ACHLCY_002906"
     CDS             complement(13948..14379)
                     /gene="osmC"
                     /locus_tag="ACHLCY_002906"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP0370123.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14725..14940
                     /gene="bdm"
                     /locus_tag="ACHLCY_002907"
     CDS             14725..14940
                     /gene="bdm"
                     /locus_tag="ACHLCY_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP0370124.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15042..15179
                     /gene="sra"
                     /locus_tag="ACHLCY_002908"
     CDS             15042..15179
                     /gene="sra"
                     /locus_tag="ACHLCY_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP0370125.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15336..17033
                     /gene="maeA"
                     /locus_tag="ACHLCY_002909"
     CDS             15336..17033
                     /gene="maeA"
                     /locus_tag="ACHLCY_002909"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0370126.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17167..18177
                     /gene="adhP"
                     /locus_tag="ACHLCY_002910"
     CDS             17167..18177
                     /gene="adhP"
                     /locus_tag="ACHLCY_002910"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP0370127.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18324..18608
                     /locus_tag="ACHLCY_002911"
     CDS             18324..18608
                     /locus_tag="ACHLCY_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0370128.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18659..19312)
                     /gene="fdnI"
                     /locus_tag="ACHLCY_002912"
     CDS             complement(18659..19312)
                     /gene="fdnI"
                     /locus_tag="ACHLCY_002912"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP0370129.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19305..20189)
                     /gene="fdnH"
                     /locus_tag="ACHLCY_002913"
     CDS             complement(19305..20189)
                     /gene="fdnH"
                     /locus_tag="ACHLCY_002913"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP0370130.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20202..23249)
                     /gene="fdnG"
                     /locus_tag="ACHLCY_002914"
     CDS             complement(20202..23249)
                     /gene="fdnG"
                     /locus_tag="ACHLCY_002914"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22662..22664),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0370131.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23481..24362
                     /gene="yddG"
                     /locus_tag="ACHLCY_002915"
     CDS             23481..24362
                     /gene="yddG"
                     /locus_tag="ACHLCY_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP0370132.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5987 a         6202 c         6184 g         6078 t
ORIGIN      
        1 taccaggttg ccgcttatct ggcggatgaa atcgccaaac tggggccgta tgagttcatc
       61 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      121 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      181 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      241 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      301 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacatacc
      361 tgataacgtt taacggtaac ggtgtcccga aacgaacccg tttcgggaca atttccaaag
      421 tctgttcact ggcattagca acggaaaata ttgttctgaa tacgcttcag aacaaaacag
      481 gtgcggttcc gacaggaata ccgttttagg gggataatat ggctacatca gtacagacag
      541 gtaaagctaa gcagctcaca ttactcggat tctttgccat aacggcatcg atggtaatgg
      601 ctgtttatga ataccctacc ttcgcaacat cgggcttttc attagtcttc ttcctgctat
      661 taggcgggat tttatggttt attcccgtgg gactttgtgc tgcggaaatg gccaccgtcg
      721 acggctggga agaaggtggt gtcttcgcct gggtatcaaa tactctgggg ccgagatggg
      781 gatttgcagc gatctcattt ggctatctgc aaatcgccat tggttttatt ccgatgctct
      841 atttcgtgtt aggggcactc tcctacatcc tgaaatggcc agcgctgaat gaagacccca
      901 ttaccaaaac tattgcagca ctcatcattc tttgggcgct ggcattaacg cagtttggtg
      961 gcacgaaata cacggcgcga attgctaaag ttggcttctt cgccggtatc ctgttacctg
     1021 catttatttt gatcgcatta gcggctattt atctgcactc cggtgccccc gttgctatcg
     1081 aaatggattc gaagaccttc ttccctgact tctctaaagt gggcaccctg gttgtatttg
     1141 ttgccttcat tttgagttat atgggcgtag aagcttccgc aacccacgtc aatgaaatga
     1201 gtaacccagg gcgcgactat ccactggcta tgttactgct gatggtggcg gcaatctgct
     1261 taagctctgt tggtggtttg tctattgcga tggtcattcc gggtaatgaa atcaacctct
     1321 ccgcaggggt aatgcaaacc tttaccgttc tgatgtccca tgtggcaccg gaaattgagt
     1381 ggacggttcg cgtgatctcc gcactgctgt tgctgggtgt tctggcggaa atcgcctcct
     1441 ggattgttgg tccttctcgc gggatgtatg tcacagcgca gaaaaacctg ctgccagcgg
     1501 cattcgctaa aatgaacaaa aatggcgtac cggtaacgct ggtcatttcg cagctggtga
     1561 ttacttctat cgcgttgatc atcctcacca ataccggtgg cggtaacaac atgtccttcc
     1621 tgatcgctct ggcgctgacg gtggttattt atctgtgtgc ttatttcatg ctgtttattg
     1681 gctacattgt gttggttctt aaacatcctg acttaaaacg cacatttaat atccctggtg
     1741 gtaaaggggt gaaactggtc gtggcaattg tcggtctgct gacttcaatt atggcgttta
     1801 ttgtttcctt cctgccgccg gataacatcc agggtgattc taccgatatg tatgttgaat
     1861 tactggttgt tagtttcctg gtggtacttg ccctgccctt tattctctat gctgttcatg
     1921 atcgtaaagg caaagcgaat accggcgtca ctctggagcc aatcaacagt cagaacgcac
     1981 caaaaggtca cttcttcctg cacccgcgtg cacgttcacc acactatatt gtgatgaatg
     2041 acaagaaaca ctaaccatgt ctgaatggtt ataagacaag ggagcgataa gtcatcgctc
     2101 cctttttcgt gcttgctgtc gtattgactt aaccgggata agtacgagaa tgagcgcaca
     2161 tctgtttacc ggaaaccagc acatatggat atctgctccc gaaacaagaa attaacgatt
     2221 agaagaccag cgatactagt tgcactggca cttttactgt gtagttgtaa aagcacgcct
     2281 ccagagtcca tggtgacacc accagcaggt tcaaagccac cagccacgac gcaacaatcg
     2341 tcacaaccga tgcgtggcat ctggctggcc acggtttctc ggctcgactg gccaccggtt
     2401 tcctcggtta acattagtaa ccccaccagc cgggcccgtg tacaacaaca ggcgatgatc
     2461 gacaaactgg atcatctgca acgtctcggc ataaacacgg tctttttcca ggtcaagccg
     2521 gacggtaccg ccctgtggcc atcgaaaatt ttgccgtggt ccgatcttat gaccggtaag
     2581 attggtgaaa atccgggtta cgatccgctg caatttatgc tcgacgaagc ccacaagcgt
     2641 gggatgaaag tacacgcctg gtttaacccc tatcgcgtat cggttaatac gaagcccggt
     2701 actatcaggg aactgaatag cactctgcct caacaaccgg cgagcgtcta tgtgcaacac
     2761 cgcgactgga tcagaacgtc tggcgatcgc tttgtcctcg acccgggcat ccctgaggtt
     2821 caggactgga tcacatcaat agtcgcagaa gtggtttccc gctatcccgt agatggcgtg
     2881 cagtttgacg actatttcta tacggagtca ccgggttcac ggctaaatga taacgaaacg
     2941 taccgtaaat acggaggcgc atttgcgtca aaagcagact ggcggcgcaa caatactcag
     3001 cagttaattg caaaggtatc gcacaccatt aaaagcatta agccgggagt cgaatttggt
     3061 gttagcccgg caggcgtgtg gcgtaaccga tcacacgatc cgctcggttc cgatacccga
     3121 ggcgcggcag cctatgacga atcctacgct gacacccgtc gatgggtgga acaaggattg
     3181 ctggattaca ttgctcccca aatttactgg ccgttctcac ggagtgccgc gcgttatgac
     3241 gtgttggcaa aatggtgggc ggatgtcgtt aaaccgacca ggacccgcct gtatatcggt
     3301 atcgccttct ataaagtggg tgaaccttca aagatagagc cagactggat gattaacggc
     3361 ggcgtacagg aactgaaaaa gcagctcgat cttaacgatg cggtaccaga aattagcggc
     3421 accatcttgt tccgtgagga ctatctgaat aaaccacaga ctcaacaagc ggtcagctat
     3481 ctgcaaagtc gttggggcag ttaaactccc attcttactt tgtaacaagc cggtgcttta
     3541 cccaccggct tgttaccctc ttgaactatc ccactactaa tcatgcttac taaagtcaaa
     3601 ttaaccacgc aattaaccac acttagttaa acagccataa atcccctaat aaaaatgtat
     3661 gaatatgaaa aaacatacga tattcgctgt caccgaaaaa taattacgat gggctatact
     3721 cttctgaaga acagtagtgt agattaaaac tgtcctaagt cgcttatttc ttttcagtat
     3781 atcttcatat ttcaggagaa tatggtgcag ttagtcgatc tcgcacgttg cgtttcgttt
     3841 tgattccgtc tgatttccca gccttataag ggtgtgaatt atggagatgt attttaaaag
     3901 aatgaaagat gagtggaccg gacttgtcga acaggcagat cctctcattc gtgctaaagc
     3961 cgcagaaatt gccgttgcgc atgctcatta tctgagtatc gagttttatc gaattgtccg
     4021 catcgacccg catgccgaag aattcttgag taatgaacaa gttgagcggc agttgaaaag
     4081 tgcgatggaa cgctggatta ttaacgtgct ttctgcccag gttgacgatg tcgaaaggct
     4141 aatacaaatc cagcataccg tcgcggaagt gcatgcccgc ataggaattc cggtagaaat
     4201 tgtcgagatg gggtttcggg tgctgaaaaa aatcctctat ccggtaatct tctcttcgga
     4261 ttattctgcc gcagaaaaac ttcaggtcta ccatttctcg attaacagta ttgatatcgc
     4321 tatggaagtg atgacccgcg cgtttacctt tagtgacagt agtgcctcaa aggaagatga
     4381 aaactatcgt atcttctcgt tactagaaaa cgccgaagaa gaaaaagaac ggcaaatagc
     4441 ctcaatactt tcatgggaaa tagatattat ctataaaatc ctgctggatt ctgatttagg
     4501 cagtagtttg cctttaagcc aggctgattt tggcctgtgg tttaaccata aaggtcgaca
     4561 ttatagtagg ccatatctcc cgtctgattc aggatttcga cggtatattc aatcaaacca
     4621 tgcgtaacac caggaatttg aataacagaa gtctgcgggt gaaattttta ttacagataa
     4681 gaaatactgt atcgcaaatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc
     4741 acgaagtcgg tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct
     4801 tcaaacgcga aattgcccat gccaaccgga ccggtacacc actgtcagtg ctgattattg
     4861 acgttgataa attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc
     4921 tgcgtaaagt ctctcaggcc ttttatgaca acgtccgcag tagtgattat gtttttcgct
     4981 acgggggcga tgaatttatc attgttttga ctgaagcttc tgaaaacgaa acgttacgta
     5041 ccgcagaacg tattcgcagt cgggtggaga aaaccaaact gaaagccgca aacggcgagg
     5101 atattgccct ctcactttcc atcggtgccg ccatgtttaa tggtcatcct gactatgagc
     5161 gcctcattca aatagccgat gaagctctgt atatcgccaa aagacgaggt agaaaccgtg
     5221 ttgaactctg gaaagccagt ctttagatgc gccaggatgc agaggtaatc atgaagctaa
     5281 ccgatgcgga taatgccgcc gatggcattt ttttccccgc ccttgagcaa aatatgatgg
     5341 gtgcggtgtt aattaacgaa aatgatgaag tgatgttttt caaccccgcc gcagagaagc
     5401 tctgggggta caaacgtgaa gaagtcattg gcaataacat tgatatgctg attccgcggg
     5461 atttgcgtcc tgcgcatcct gaatacattc gtcataaccg tgaaggcggt aaagcgcgtg
     5521 tcgaggggat gagtcgggag ctgcagctgg agaaaaaaga cggcagtaaa atctggaccc
     5581 gttttgcgct atcgaaagtg agcgccgagg ggaaagttta ttacctggcg ctggtacggg
     5641 atgccagcgt agaaatggcg caaaaagaac agactcgaca attgattatt gccgttgacc
     5701 atctcgaccg accggtgatt gtcctcgatc cggaacgcca tattgtgcag tgcaatcgcg
     5761 catttaccga aatgtttggt tactgcatta gcgaagccag cggtatgcag cccgatacac
     5821 tcctgaacat tcctgaattc cctgccgata accgcattcg tttacaacag ttgctatgga
     5881 aaaccgcccg cgatcaggac gaatttctgc tgttgacgcg caccggtgaa aaaatctgga
     5941 ttaaagcctc tatcagcccg gtttatgacg tgctcgcgca tctgcagaac ctggtaatga
     6001 ctttctcgga tatcaccgaa gaacggcaaa tccgccagct tgaaggcaat attctcgccg
     6061 ccatgtgcag cagcccgcca tttcatgaaa tgggggaaat catttgtcgt aacatcgaat
     6121 ctgttctcaa cgaatcgcat gtttcgctgt tcgcactgcg caacgggatg ccgatacact
     6181 gggcgtcatc ttcgcacggt gcagaagttc aaaatgcgca aagctggtca gcgaccattc
     6241 gtcagcgtga tggcgcgcct gcggggatcc tgcaaattaa aacctcgtca ggagcagaaa
     6301 ccagcgcctt tatcgaacgc gtggcagata tcagtcagca tatggccgcg ctggcgctgg
     6361 aacaggaaaa aagccgccag catattgaac aactcatcca atttgatccg atgaccggtc
     6421 tgccaaatcg caagaacctg cacaattacc tcgatgacct ggtcgacaaa gccgtctctc
     6481 cggtggtgta tctcatcggt gttgaccata ttcaggatgt gattgatagc cttggctatg
     6541 cgtgggccga tcaggcattg ctggaagtgg tcaatcgctt tcgtgaaaaa ctcaaaccgg
     6601 atcagtatct ctgtcgtatc gaaggtacgc agtttgtcct cgtgagcctg gaaaacgacg
     6661 tcagtaacat tacccagatc gccgatgagc tacggaatgt ggtcagcaag ccgataatga
     6721 ttgacgataa accattcccg cttaccttga gtattggcat cagctacgac gtgggtaaaa
     6781 accgcgatta cctactctcc actgctcaca atgcaatgga ttatattcgc aagaatggcg
     6841 gtaacggctg gcagttcttc agcccggcga tgaacgaaat ggtaaaagag cgtttggttt
     6901 taggcgcagc gctgaaagaa gcgattagca ataaccaact gaaactggtt taccagccgc
     6961 aaatcttcgc agaaacgggt gaactgtacg gcatcgaagc ccttgctcgc tggcacgatc
     7021 cccagcatgg tcatgtaccc ccttcacggt ttattcctct cgcagaagag attggtgaaa
     7081 tcgaaaatat tgggcgctgg gtcatcgcgg aagcttgccg tcagttagca gaatggcgta
     7141 gccagaatat tcatatcccg gcgttatccg tgaacttgtc ggcgctgcac tttcgcagta
     7201 atcagctgcc taatcaggtg tctgatgcaa tgcaagcctg gggtattgac ggccaccagc
     7261 tgacggtaga aatcacggaa agcatgatga tggaacacga taccgaaatc tttaagcgca
     7321 ttcagatcct gcgtgatatg ggcgtgggct tatcggtaga tgattttggt acgggctttt
     7381 ccggattatc ccgcttagtc agtcttccgg taacggaaat caaaattgac aaaagttttg
     7441 tcgatcgttg tctgaccgaa aaacgcatcc ttgccttact tgaagccatt accagcattg
     7501 ggcaaagcct caatttaacc gtcgtggcgg aaggcgtcga aatcaaagag caatttgaga
     7561 tgctacgcaa gatccactgt cgcgttattc agggatattt cttttcccgc cccctacccg
     7621 ccgaagaaat tccaggctgg atgagcagcg tgttaccgct gaaaatctga caaattcctc
     7681 tcgcccgcac tcgcgggttt tcatttaacg tgacactgtc acttaaatac tgtgatttca
     7741 gctacgattc cgggagattc cttccttaac ttcccctctt gcaccaaaac tgcactacgc
     7801 tgcacaaatg ttgatcaaaa gttaaaaaat ataactttaa gccagcattc tggcgcttat
     7861 cccggcatgg catgagatct gcataagcgg aaagcgcagc aatttttgtc ttatacagga
     7921 accgtttatg tcggatacca ccgaactggt tgatttagcc gtaatcttcc ctgatctgga
     7981 gatcgaattg aaatacgcct gcgctgataa catcacaggt aaagctattt atcagcaagc
     8041 gcgttgtctg ctacacaagg atgcgattac cgcgctggcg aaaagtatca gtatcgccca
     8101 gctgtcaggc ttacaactgg tgatttacaa tgcgtatcgc ccacaacaag cacaggcgat
     8161 gttgtggcaa gcctgcccag acccgcaata tgttgttgat gtgacggtca gttctaatca
     8221 cagccgtggc acggcgatcg acctgacgct tcgtgatgag cacgggaaca tcctcgatat
     8281 gggcgcaggg ttcgatgaaa tgcacgagcg ctcccatgcc tatcaccctt ccgtcccgcc
     8341 cgccgctcag cgcaatcggc tgttgctgaa tgcgataatg actggtggcg gttttgtcgg
     8401 tatctccagc gaatggtggc acttcgaatt accccaggca gcgagttacc ctctgctcgc
     8461 tgatcaattc tcttgtttta tatcccctgg cacacagcac gtcagttaat caggaaactg
     8521 tcatgaagag atcgatatcg tttcgtccca cattgctcgc gctcgtcctt gccacaaatt
     8581 tcccggtttc gcacgccgcc gtaccgaaag atatgctggt gattggtaag gccgccgatc
     8641 cacaaaccct cgacccggcg gtaacaatag ataataacga ctggacggtg acctacccgt
     8701 cttatcagcg gctggttcag tacaaaacgg acggtgataa aggctcaacc gacgttgaag
     8761 gcgatctggc aagtagctgg aaagcgtctg acgatcaaaa agagtggacg ttcaccctga
     8821 aagataatgc taaatttgcc gatggcacac ctgtcactgc cgaagcagta aaactttctt
     8881 ttgagcggct actaaaaatc ggccaggggc cagcagaagc atttcccaaa gatttaaaga
     8941 ttgatgctcc cgacgaacat acggtgaagt ttacccttag ccaaccattc gcaccgttcc
     9001 tctacacgct ggcgaatgac ggtgcatcca ttatcaatcc ggcggtctta aaggaacatg
     9061 cagcggatga tgctcgcggc ttcctcgcgc aaaataccgc cggttccgga ccatttatgc
     9121 tgaaaagctg gcaaaaaggt cagcaattag ttctggtgcc aaatccgcat taccccggca
     9181 ataaaccgaa ctttaagcga gtatcggtaa aaattatcgg tgaaagtgcc tcccgtcgcc
     9241 tgcagctctc ccgtggcgac attgacattg ccgatgcgct gccggtggat caactcaacg
     9301 ccctgaagca ggaaaataaa gtcaatgtgg cagagtatcc gtcactgcgc gttacctatc
     9361 tgtatctgaa taacagcaaa gcgcctctta atcaggcgga tctacgtcgg gccatttcct
     9421 ggtctaccga ttatcagggc atggttaacg gcattctgag tggtaacgga aaacagatgc
     9481 gcggcccgat tccggaaggc atgtggggct acgatgcgac ggcaatgcaa tacaaccatg
     9541 acgaaacgaa agccaaagcc gaatgggata aagtgacgag caaacccacc agcctgacgt
     9601 ttctctactc cgataacgat ccgaactggg agcctattgc tttggcgaca caatccagtc
     9661 tcaacaagct gggtatcaat gtgaagctgg aaaagctggc gaacgccacc atgcgcgaca
     9721 gagtgggtaa aggtgattac gacattgcga ttggcaactg gagtccggat tttgccgacc
     9781 cgtatatgtt tatgaattac tggtttgagt cagacaaaaa aggtctgccg ggtaaccgct
     9841 cgttctatga aaacagtgag gtcgataagt tactgcgcaa tgcgcttgcg accaccgacc
     9901 agacgcagcg tacccgggac taccagcagg cacagaaaat cgtcattgat gacgctgctt
     9961 atgtgtacct gttccagaaa aactaccaac tggcgatgaa caaagaggtg aaaggctttg
    10021 tgttcaatcc catgctggaa caggtcttca atatcaatac catgagtaaa taacgtgacc
    10081 ttctggagta ttttacgcca acgctgctgg gggctggtgc tcgtggtggc gggcgtctgc
    10141 gtgattacgt ttattatctc gcatctgatc cctggcgatc cggcgcggtt actggcgggt
    10201 gaccgcgcca gcgatgctat cgtggaaaat attcgccagc aactgggact ggaccagcca
    10261 ctgtatgtac agttttaccg ctacgtcagc gacctgtttc atggtgacct gggaacatcc
    10321 attcgtaccg ggcgtccggt gctggaagag ttgcgtatat ttttcccggc gacgctggag
    10381 ctggcttttg gtgccctgct gctggcactc ctgattggca tcccgctggg catactctct
    10441 gcagtctggc gaaatcgctg gctggatcat ctggtgcgaa taatggccat taccggaatc
    10501 tccacacctg cgttctggct tgggctgggc gtcattgtgc tgttttatgg tcatctgcaa
    10561 attcttcccg gcggcggaag gcttgatgac tggctggatc caccaacgca cgttaccggc
    10621 ttttatctgc tcgatgcgct gcttgaaggc aacggtgaag tcttcttcaa tgcgttgcaa
    10681 catctcatct taccggcatt aacgctggcg ttcgttcacc tgggaattgt cgctcgccag
    10741 atccgctcag cgatgctgga acaattgagt gaagactaca ttcgtaccgc ccgggccagc
    10801 ggcttgcccg gctggtatat cgttttatgt tatgcgctac ccaatgcgtt gatcccatcg
    10861 attaccgtat tgggtttggc gctgggcgat ttgttgtatg gcgcagtgct caccgaaacc
    10921 gtttttgcct ggcccggaat gggtgcatgg gtagtaacat caatacaggc gctcgacttc
    10981 ccggcagtga tgggctttgc cgtcgtggtt tcatttgctt atgtgctggt caacctggtg
    11041 gtggatttgc tctatttgtg gattgatccg cgtatcggac gtggaggtgg tgaatgatgc
    11101 taagcgagga aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac ggtgcaaaac
    11161 tggtatggat gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc attgtattaa
    11221 tgctattgat gatgattttt tcaccgtggc tggcgacgca tgatcccaac gccattgatt
    11281 taaccgcccg ccttttgccg ccttctgcgg cgcactggtt tggcaccgat gaagtgggac
    11341 gcgatctgtt cagccgcgta ctggtcggca gtcagcaatc aattctcgcc ggattagtcg
    11401 tggttgccat tgcgggtatg attggttcgc tactcggatg tctatccggt gtgcttggcg
    11461 gacgcgcaga cgccatcatc atgcgcatca tggacattat gctgtcgatt ccttcgttgg
    11521 tactgacaat ggcactggct gccgctctcg ggccgagttt atttaacgcc atgctggcga
    11581 ttgctattgt gcgaattccc ttttatgtgc gcctggcgcg ggggcaagca ttagttgtac
    11641 gccagtatac ctatgttcag gcggcgaaaa cctttggtgc gtcgcgttgg catctgatca
    11701 gctggcatat tttacgtaac tccctgccgc cgctgatcgt gcaggcatcg ctggatatcg
    11761 gtagcgcgat tttaatggcc gccacgttgg gatttattgg cctgggtgct caacaaccga
    11821 gtgctgaatg gggcgcgatg gtagcgaatg gtcgcaacta tgtactcgat cagtggtggt
    11881 attgcgcatt tccgggggca gcgattttgc ttaccgccgt tgggtttaat ctctttggtg
    11941 atggtattcg cgatctgctt gacccgaaag caggaggaaa gcagtcatga cccaacccgt
    12001 tctggacatt caacagctgc atttgagttt ccccggtttt aacggtgatg ttcacgcgct
    12061 caacaatgtg tccttgcaga ttaaccgcgg cgaaattgtc ggtctggtgg gagaatccgg
    12121 ctcaggtaaa tcagtcaccg caatgctgat tatgcgtctg ctacagacgg gcagttattg
    12181 cgtacatcgg gggcagattt cactgctggg agacgatgtt cttaacgctc gggaaaaaca
    12241 gcttcgtcag tggcgcggcg cacgagtggc gatgatcttt caggaaccga tgaccgccct
    12301 caatccgaca cttcgaatag gtcttcagat gatggacgtg atccgccatc atcaaccaat
    12361 aagtcgtcgg gaagccagag ctaaagcgat tgccctgctg gaagagatgc aaatcccgga
    12421 tgccgtggaa gttatgtcgc gctatccgtt tgagctttca ggtggtatgc gccagcgggt
    12481 aatgattgcg ctggcattct cctgcgagcc gcaattgatt attgccgacg aaccgactac
    12541 ggcgctggac gtcacggtgc agttgcaggt actgcgtttg cttaaacata aagcccgcgc
    12601 cagtggaact gcggttctgt tcatcagcca tgatatggcc gtggtgtcgc aactgtgcga
    12661 tagcgtttac gtgatgtatg ccggaagcgt aattgaaagc ggcgtgacgg cagatgttat
    12721 ccatcatccc cggcatccgt ataccattgg tttgctccaa tgcgcaccag aacatggagt
    12781 accacgccag ctattacccg ccattccagg gacggtacca aacctcaccc atttgcctga
    12841 aggctgcgct tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa acgtcccggc
    12901 gctgacagcg tgtggtgaca acaaccagcg ctgcgcctgt tggtatccgc agcaggaggt
    12961 cataagtgtc tgacacgtta ttaacgttac gcgacgtcca tatcaatttc ccggcccgta
    13021 aaaactggct tggtaaaact acggaacatg ttcatgccat taatggtatt gatttacaga
    13081 tccgccgtgg tgaaacctta gggatcgtcg gcgagtcagg ctgcggcaaa agcaccctcg
    13141 cacagctttt aatgggcatg ctgcaaccga gccacgggca gtacatccgt tctggctcac
    13201 aacgcattat gcagatggtg tttcaggacc cgctctcttc gctcaatccg cgcttaccgg
    13261 tgtggcgcat catcacagaa ccgctctgga tagctaagcg tagtagtgaa caacagcggc
    13321 gagcgttggc agaggagctg gctgtgcagg tgggtattcg tccggagtat ctcgaccgcc
    13381 tgcctcatgc gttctccggc gggcagcggc aacgcatcgc cattgccaga gcactctctt
    13441 cgcagcctga cgtgattgtg cttgatgagc caacctcggc gctggatatc tccgtgcagg
    13501 cgcagatcct caatttactg gtaacgctac aggaaaatca cgggctgact tatgtgctga
    13561 tttcacacaa tgtctcggtg atacgtcata tgagcgatcg ggtggcggtg atgtatctcg
    13621 ggcagattgt ggaactaggg gaagcgcaac aggtgttgac tgctccggca catccatata
    13681 cccgattatt gctggattcc ctccccgcca ttgataaacc gctggaggaa gaatgggcat
    13741 tacgtaaaac ggatctgccg ggaaaccgca cgttgccgca aggctgtttt ttctacgaac
    13801 gttgccctct ggcaacccac ggatgtgaag tccggcaatc attaacaata agggaggacg
    13861 gacgtgagat ccggtgctgg cgggcgctgt agagatgcta acaacgcatc aggcattcac
    13921 gcgcctgacg cgtcatccgg caatgcttta cgatttcaac tggtaatcaa gcgtaatttc
    13981 cgctttcagt acctgagaaa ccgggcatcc tgcttttgct ttctggatga tgccgtcaaa
    14041 ggtagaggca tcaatacccg gcaccgcaac ttcactcttc agtgcgattt tcgtaatcgc
    14101 aaaaccggca tccactttat ccagcgacac atcggcggtg gtatcaatcg atgttggcgt
    14161 aaatcccgct tcccccagca ttaatgaaag cgccattgag aaacatgcgg catgcgctgc
    14221 gccaatcagt tcttcagggt tagttccttt ttcgccttca aaacgcgtgt taaatccata
    14281 cggctgttgg ttcagcacac cactctcggt ggatactgtt cccttcccgc gtttgatatc
    14341 gccttcccag tgtgcctgac ctttcttatg gattgtcatt gttgctctcc tgtgggctta
    14401 aaaaggaaat tatagactac gtgagaaaca gcacgaggat aagcaggata ttccgaataa
    14461 aaatccggca gaaatattac ttctgcactt aaattaaatt tccacaatag ataaccggga
    14521 atatataacc caagagatat tgcattaata ttaatcaact caccacaata ataataccta
    14581 tcaatatcat gaggttaaaa tataaaaatc atcaaccagg actaatctta acaacgaagc
    14641 ggcaaatatt tgccatgctg aatgtgcttt ataaatctgc gatccgtagc agacaccata
    14701 aatacacaga cacggagaat cactatgttt acttattatc aggcagaaaa ttcaacagca
    14761 gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcacgg tgttgtcact
    14821 gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa cgacatcctc
    14881 agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc tacgctttaa
    14941 ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct tcgctacggc
    15001 gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg aaccgtcagg
    15061 cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata gttaccgaag
    15121 gtgacaaatc cagcgtggta aataatccaa ccggcagaaa acgccccgct gaaaagtaat
    15181 tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg gtgtttttgt
    15241 ttttatctgc tctatacttg gggccgacgc cctggcggta aagcaaagac gataaaagcg
    15301 tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca aaaaaacagc
    15361 gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg ttgaataaag
    15421 gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta ctgccggaag
    15481 tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag ggattcaaaa
    15541 ccgaaatcga caaacacatc tacctgcgta acatccagga caccaacgaa accctcttct
    15601 accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc ccaaccgtcg
    15661 gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg tttatctctt
    15721 accagaaccg gcacaatatg gacgatattc tgcaaaacgt gccgaaccat aatattaaag
    15781 tgattgtggt gactgacggt gaacgtattc tggggcttgg tgaccagggc atcggcggga
    15841 tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc agcccggcgt
    15901 atacccttcc ggtggtgctg gatgtcggaa cgaacaacca acagctgctt aacgatccgc
    15961 tgtatatggg ctggcgtaat ccacgtatca ctgacgatga atactatgaa ttcgttgatg
    16021 aatttatcca ggctgtgaaa caacgctggc cggacgtgct gttgcagttt gaagactttg
    16081 ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt tcttttaacg
    16141 atgacattca gggcactgcg gcagtaacag tcggcacact gatcgcagcc agccgcgcgg
    16201 caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca gcgggatgcg
    16261 gcattgccga aatgatcatc gcccagaccc agcgcgaagg attaagcgag gaagcagcgc
    16321 ggcagaaagt ctttatggtc gatcgctttg gcctgctgac ggacaagatg ccgaacctgc
    16381 tacctttcca gaccaaactg gtgcagaagc gcgaaaacct cagtgactgg gataccgaca
    16441 gcgatgtgtt gtcactgctg gatgtggtgc gcaatgtaaa accagatatt ctgattggcg
    16501 tctcaggaca gaccgggctg tttacggaag agatcattcg tgagatgcat aaacactgtc
    16561 cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc acaccgcagg
    16621 acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac tggcagcccg tttaatccag
    16681 tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt attttcccag
    16741 gcatcgggct gggtgttatt gcttccggcg cgtcacgtat caccgatgag atgctgatgt
    16801 cagcaagtga aacgcttgct cagtattcgc cgctggtgct gaacggcgaa ggtctggtac
    16861 taccggaact gaaagatatt cagaaagtct cccgcgcaat tgcgtttgcg gttggcaaaa
    16921 tggcgcagca gcaaggcgtg gcggtgaaaa cgtctgccga agctctgcaa caagccattg
    16981 acgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc taagcctgcg
    17041 cccggtagtg aaggctaccg ggctatttcc ctctcccttt ttcagatctc atccatactg
    17101 ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat ccgaaaagga
    17161 ggaactatga aggctgcagt tgttacgaag gatcatcatg ttgacgttac ggataaaaca
    17221 ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg tgtatgtcat
    17281 accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ctggggtaat tctgggccat
    17341 gaaggcatcg gtgtggtggc agaagtgggt ccaggtgtca cctcattaaa accaggcgat
    17401 cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg taacagtggt
    17461 aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg cgggatggcg
    17521 gaagagtgca tcgtggtcgc cgattacgcg gtaaaagtgc cagatggtct ggactcggcg
    17581 gcggccagca gcattacctg tgcgggggtc accacctaca aagccgttaa actgtcaaaa
    17641 attcgtcccg gtcagtggat tgctatctac ggtcttggcg gtctgggtaa cctcgccctg
    17701 caatacgcga agaatgtctt taacgccaaa gtgatcgcca ttgatgtcaa tgatgagcag
    17761 ttaaaactgg caaccgaaat gggtgcagat ttagcgatta actcacgcac cgaagacgcc
    17821 gccaaaattg tgcaggagaa aaccggtggc gctcacgctg cggtggtaac agcagttgct
    17881 aaagctgcgt ttaactcggc agttgatgct gtccgtgcag gcggtcgtgt tgtggctgtc
    17941 ggtctgccgc cggagtctat gagcctggat atcccacgcc ttgtgctgga tggcattgaa
    18001 gtggtcggtt cgctggtcgg cacgcgccag gatctaaccg aagccttcca gtttgccgcc
    18061 gaaggcaaag tggtgccgaa agtcgccctg cgtccgttag cggacatcaa caccatcttt
    18121 accgagatgg aagaaggcaa aatccgtggc cgtatggtga ttgatttccg ccgttaagag
    18181 gcctttgctg cgagtgccag tcctggtgtc gcagcatcgc actctctccg acattaaatg
    18241 gctaaatgga ttattgttcc ttcctacgca acaataatga tagtgttcct gatgaaggaa
    18301 cactactgag agtaaagaca ctgatgaaaa tggccaatca tccccgcccg ggggacatta
    18361 ttcaggaatc actggacgaa cttaatgtca gcctgcgcga gtttgccaga gcaatggaaa
    18421 ttgctccctc aacggcaagc cgattgctga ccggaaaagc agctttgaca ccagaaatgg
    18481 cgattaaact ttccgtggtg atcggcagtt cgccgcaaat gtggctgaat ctgcaaaacg
    18541 cctggagtct ggcagaggca gaaaaaacgg tggatgtgtc gcgactacgc cgtctggtta
    18601 cgcaataaaa aaatggcgct tcttaaagcg ccattttcag cttaaagttc aaaagagatt
    18661 atatcccttc ttcactctct tttttcgctt ctgccttctc gatttcacga taccagcgcg
    18721 gatggtgttt cttcgcccag cgacggctca ccttcccttc gatcatccct ttaatcgacc
    18781 ctttcaccca gaatgccata tacatatgga tcaggatggc gtggatcagg atgatacccg
    18841 cagccgcgtg gatcagcagg ctgtagcgaa caacctgcat cgggaagtac tgcgcaaagt
    18901 acggacgcca gataatcacc ccggtcacca gcagcacgaa aatcatgctc atgatcgacc
    18961 agaacatcat cttttgcccg gcgttgtact taccgacatc cgccacttta tgctcattgc
    19021 ctttcaatac ttcgacaatg ttcaacagcc acggaatatc tttcttatcc gggatgttgt
    19081 gatgcacaaa acgcacaaac ataaacatca gtgcgacgaa aatcgcaatg ccgaaaaacg
    19141 ggtgcaaaat gcgtcccatc tgcggcgtac cgaaggtttg cgtcagccat tgcagcgtcg
    19201 ggaagaagaa cgaaatcccg gacagcgcca ccaggaagaa gcaaatcacc acggtccagt
    19261 gacaggcgcg atcaataaat ttggtgcgca caatcatttt cgacttactc atgatgatcc
    19321 tcctcgtcat cgtccacttc cttattcggg ccaataccga tgtagtggaa aatcaacccg
    19381 gcaaaagtgg caataaagcc agccgctgcc agcggtttca acgcgccttt ccacaggctt
    19441 accgaggtgt cgatcttcgg atctttcggc agaccgtgat acagctccgg ctgatcggcg
    19501 tgatgcagca cgtacataac gtgcgtacca ccgacccctt ccgggttgta gacgccagca
    19561 tgttcgtaac cacgcgcttt cagtttcgcc acgcgctgtt ccgccagctc cagcatctcc
    19621 ttcttggtgc cgaagtggat agccccggtc ggacaggttt tcacacaagc cggttcctgg
    19681 ccgacgctga cgcgatcgac gcagagcgtg catttatata cccggttatc ctctttgttg
    19741 aggcgcggaa tattaaacgg acacccggca atgcagtaac cacagccgat gcagttttcc
    19801 gactggaaat cgacaatccc gttagcgtac tgaatgattg caccagcaga cgggcacgcc
    19861 ttcaggcagc cgggatcttc acagtgcata cagccgtctt tacggatcag ccactccagc
    19921 ttgccgttct gttcggtttc gctaaagcgc atcaccgtcc aggacttggc gctcagatcg
    19981 gcggggttat cgtaaacccc gacgcagtgc cccacttcat cacggatgtc gttccactcc
    20041 gaacacgcca cctgacaggc tttacagccg atacaggtgg aaacgtcgat aagttttgcg
    20101 acttctgctt tgtaatcacg cacctgagaa ggcggcgtga tggagttagt tgcggacctt
    20161 ttgataatgt cctgcgtttc catagccatc tgttcgcccc cttacgcctt ctcgatgttg
    20221 actaagaacg ctttatattc cggcgtttgc gagtttgcat caccgacatt cggcgtcaga
    20281 gtgttagcga tataaccttt acgcgcgaca ccctcaaagc cccagtggat tggaataccc
    20341 accgtttcaa cctgctgacc atttacattc agtggtttca gacgacgcgt taccacagcc
    20401 accgcgcgga taaagccacg cttgcttgag acagtgacac gatcgccatt attaatgcct
    20461 ttcgccgccg ccagcgtttc gctgatttcc acaaactgtt ccggctgagc aattgcgttg
    20521 agcaatgcgt gcttggtcca ggtgtggaag tgctcggtca gacgataggt cgtacccaca
    20581 tacgggaact gctctttttt acccatccgc agtgcgtctt gttcatacag acgaacaacc
    20641 gggttagaca ccacgttcgg atgcagcggg ttagtgccca gcggcgtttc aatcggctcg
    20701 tagtgttccg ggaacggacc ttccgccatt ttgttgatgg caaacaggcg tcccatccct
    20761 tccggctgca tgataaacgg cccggttggc gtacccggtg cggcattgcc gaagtcagga
    20821 atatcgttac ccgtccactt gctgccgttc cactggatca gcatccgttt cggatcccac
    20881 ggtttaccgt tgatatccgc cgaagcacgg ttgtacagca cgcgacggtt gagcggccac
    20941 gcccaggccc atcccagcgt attccccaga ccggacgggt cggagttatc gcggttagcc
    21001 atctggttgc cctgctctgt ccagctaccg gtgtagatcc agcaagaaga tgcggttgta
    21061 ccgtcatcac gcagatgcgc aaagctactc agcaactgac ctttcttcgc aatcagcacg
    21121 ccattagcat catagagatc ttccagcgca tagccgttgt tctctttagc tacttcgtca
    21181 gattgcggtt cgtgcggctg cttgtagttc cagctcatct tcatcagcgg ttctacgcct
    21241 ttaccacctt cggcctggta cagctcgcgc aggtgatggt agatacccgc cagaatttca
    21301 ccgtcgttac gcgcttcgcc cggcgcatcc tgacctttcc agtgccactg cagccagcga
    21361 ccggagttag caatagaacc atcttcttca gcaaagcagg tcgaaggcag acggaatact
    21421 tcagtctgaa tagacgccgg atcgacatcg ttcgactcgc cgtggttctg ccagaaggtt
    21481 gaggtttcag tcaccagcgg atcgataacc accatgtact tcagcttgct taggcagctc
    21541 accactttgt ttttgtccgg gaaggacgca accgggttaa agccctggca gaaataaccg
    21601 gtgactttgc cttcatccat catgttgaaa tacttgatga cgtcgtaggt ctggtcccac
    21661 ttcggcagcc agtcatagcc ccagttgttc tctttctgcg cggcatcgcc atagaaagat
    21721 ttcatcaggc taacgaagaa cttcggatag ttgctccagt agttcacctg accagccagc
    21781 gtcgctttcg gcgtgttcgc ttccagatac gactgcaaat caacctgttt ttctgacggc
    21841 agcgtcagat aacctggcag gctggtagag agcagaccta agtcagtcag accctgaatg
    21901 ttggagtgac cacgcaatgc attcacgccg ccaccggcca tacccatgtt accgagcagc
    21961 aactggatca tcgccatagt acggatgttc tgcgcaccca cggtgtgctg cgtccagccc
    22021 agcgcgtaca ggaaggtggt tgtgcgatcc ggtgcgctgg tggaggccag cacttcacac
    22081 actttcagga agtcggcttt tggcgtacca cagatgtttt caacaacgtc cggcgtgtag
    22141 cgggaaacgt gcgctttcag caggttccac acacagcgcg gatgagtcag tgtttcatcg
    22201 cgtttcgcat agccgttttc atcgaactga tagttccagg acgatttatc gtattgccgt
    22261 ttttcagcgt cgtagccgct gaacagaccg tcttcgaaag caaaatcatc acgcaccagc
    22321 aggctggcgt tggtgtaatg cttaacgtat tcggcgttga ttttgtcgtt ttcgatcagg
    22381 tagcgcaaaa cgccagacag gaacgtaatg tccgtaccgg aacgaatagg tgcgtaaata
    22441 tccgccacag aagcggtacg cgtaaaacga ggatcgacaa cgatcaaggt tgcatcgttg
    22501 ttgtttttcg cttccatcgc ccagcggaaa ccgacgggat gcgcttcagc agcgttaccg
    22561 cccattacca ttacgacgtt agcgtttttg atatccaccc agtggttggt catcgcaccg
    22621 cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacgcg cgcctggttg
    22681 tctaccgcca gcatcccgag ggagcgggca aatttctgtg tcagcatccc ggtttcgttg
    22741 ctggcaccgg aggcacacag cataccggta gaaagccaac ggtttaccgt tacgccctgc
    22801 tcgttctttt caataaagtt agcgtcacgg tcagctttca tcagctttgc aatacgggag
    22861 aatgcttctt cccagctaat gcgctgccat ttgtcagaac ctggcgcacg atattccggg
    22921 tagcgcagac ggttttcgct gttgacgtaa tccagcaaac cggccccttt tgggcacagc
    22981 gcaccacggc ttaccggatg atccgggtca ccttcaatgt gataaatcgc ttctctggcg
    23041 tttttcgcgc catcacccag gctatacatc aatagcccgc aacctacgga acagtatgtg
    23101 caggtgttac ggatctcttt agcgcgtaat aatttgtagt ttcgcgcctg agccagtgct
    23161 tgcttcgggg caaagcccaa tgccgctacc gttgttccag ccataccgcc cgcgcagatt
    23221 ttaaaaaatt gtctgcgact gacgtccatt gctttcctct tttttcaggg gtattactgc
    23281 gcgaggaaac taacagtaaa attctgaatc tttttgcgcg aaatcaaaaa gaacacgtgt
    23341 ttgccacaaa ataatccgcc tgcgcaaatt gacctacctc aatagcggta gaaaaacgca
    23401 ccactgcctg acaggccagt taaaaaaatg ctataaaatt cagcttaatt tttaacggca
    23461 agagagacaa aacagcgagc atgacacgac aaaaagcaac gctcataggg ctaatagcga
    23521 tcgtcctgtg gagcacgatg gtaggattga ttcgcggtgt cagcgagggg ctcggcccgg
    23581 tcggcggcgc agctgctatc tattcattaa gcgggctgct gttaatcttc acggttggat
    23641 ttccgcgtat tcggcagatt ccgaaaggct atttactcgc cgggagtctg ttattcgtca
    23701 gctatgaaat ctgtctggcg ctttccttag ggtatgcggc gacccgtcat caggcgattg
    23761 aagtgggtat ggtgaactat ctgtggccca gcctgacaat tctctttgcc attctgttta
    23821 atggtcagaa aaccaactgg ctgattgtac caggattatt attagccctg gtcggcgtct
    23881 gctgggtgtt aggcggtgag aatgggttac attatgatga aatcatcaat aatatcacca
    23941 ccagcccatt gagttatttc ctggcgttta ttggtgcgtt tatctgggca gcctattgca
    24001 cagtaacgaa taaatacgca cgcggattta acggaattac cgtttttgtc ctgctaacgg
    24061 gagcaagtct gtgggtttac tattttctca cgccacaacc agaaatggta tttagcacgc
    24121 ccgtcatgat taaactcatc tctgcggcat ttaccttagg atttgcttat gctgcatgga
    24181 atgtcggtat actgcatggc aatgtcacca taatggcggt aggctcgtat tttacgcccg
    24241 tactttcctc agcgcttgca gccgtgctgc tcagcgcccc gctgtcgttc tcattctggc
    24301 aaggcgcgct aatggtctgc ggcggttccc tgctctgctg gctggcgaca cgtcgtggtt
    24361 aaaataattg ccgggtctac ggcccggcag ttaatcagat aaattccgac aatattaaaa
    24421 aataaatttc aaataccagt aattcacctt a
//
LOCUS       DAZGTR010000067        24012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGTR010000067 DAZGTR010000000
VERSION     DAZGTR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.067"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..3864)
                     /gene="entF"
                     /locus_tag="ACHLCY_002916"
     CDS             complement(<1..3864)
                     /gene="entF"
                     /locus_tag="ACHLCY_002916"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP0370133.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIR"
     gene            complement(3861..4079)
                     /gene="ybdZ"
                     /locus_tag="ACHLCY_002917"
     CDS             complement(3861..4079)
                     /gene="ybdZ"
                     /locus_tag="ACHLCY_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP0370134.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4082..5284)
                     /gene="fes"
                     /locus_tag="ACHLCY_002918"
     CDS             complement(4082..5284)
                     /gene="fes"
                     /locus_tag="ACHLCY_002918"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP0370135.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5527..7767
                     /gene="fepA"
                     /locus_tag="ACHLCY_002919"
     CDS             5527..7767
                     /gene="fepA"
                     /locus_tag="ACHLCY_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP0370136.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7807..7905
                     /locus_tag="ACHLCY_002920"
                     /pseudo
     CDS             7807..7905
                     /locus_tag="ACHLCY_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7942..8562
                     /gene="entD"
                     /locus_tag="ACHLCY_002921"
     CDS             7942..8562
                     /gene="entD"
                     /locus_tag="ACHLCY_002921"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP0370137.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCKQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8684..8836)
                     /gene="hokE"
                     /locus_tag="ACHLCY_002922"
     CDS             complement(8684..8836)
                     /gene="hokE"
                     /locus_tag="ACHLCY_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0370138.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9187..9339)
                     /gene="hokE"
                     /locus_tag="ACHLCY_002923"
     CDS             complement(9187..9339)
                     /gene="hokE"
                     /locus_tag="ACHLCY_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0370139.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9308..9577
                     /locus_tag="ACHLCY_002924"
     CDS             9308..9577
                     /locus_tag="ACHLCY_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370140.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            9781..10899
                     /gene="ybdK"
                     /locus_tag="ACHLCY_002925"
     CDS             9781..10899
                     /gene="ybdK"
                     /locus_tag="ACHLCY_002925"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP0370141.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10965..11213
                     /gene="ybdJ"
                     /locus_tag="ACHLCY_002926"
     CDS             10965..11213
                     /gene="ybdJ"
                     /locus_tag="ACHLCY_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP0370142.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWQRWFSLAD"
     gene            11278..11646
                     /gene="ybdF"
                     /locus_tag="ACHLCY_002927"
     CDS             11278..11646
                     /gene="ybdF"
                     /locus_tag="ACHLCY_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0370143.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11740..12393
                     /gene="nfsB"
                     /locus_tag="ACHLCY_002928"
     CDS             11740..12393
                     /gene="nfsB"
                     /locus_tag="ACHLCY_002928"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP0370144.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12501..13748
                     /gene="ybdG"
                     /locus_tag="ACHLCY_002929"
     CDS             12501..13748
                     /gene="ybdG"
                     /locus_tag="ACHLCY_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP0370145.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13816..15192)
                     /gene="pheP"
                     /locus_tag="ACHLCY_002930"
     CDS             complement(13816..15192)
                     /gene="pheP"
                     /locus_tag="ACHLCY_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP0370146.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15294..18437)
                     /gene="cusA"
                     /locus_tag="ACHLCY_002931"
     CDS             complement(15294..18437)
                     /gene="cusA"
                     /locus_tag="ACHLCY_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP0370147.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLLLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18449..19672)
                     /gene="cusB"
                     /locus_tag="ACHLCY_002932"
     CDS             complement(18449..19672)
                     /gene="cusB"
                     /locus_tag="ACHLCY_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP0370148.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(19688..20020)
                     /gene="cusF"
                     /locus_tag="ACHLCY_002933"
     CDS             complement(19688..20020)
                     /gene="cusF"
                     /locus_tag="ACHLCY_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP0370149.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20178..21551)
                     /gene="cusC"
                     /locus_tag="ACHLCY_002934"
     CDS             complement(20178..21551)
                     /gene="cusC"
                     /locus_tag="ACHLCY_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP0370150.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSIRLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            21708..22391
                     /gene="cusR"
                     /locus_tag="ACHLCY_002935"
     CDS             21708..22391
                     /gene="cusR"
                     /locus_tag="ACHLCY_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP0370151.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYQVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22381..23829
                     /gene="cusS"
                     /locus_tag="ACHLCY_002936"
     CDS             22381..23829
                     /gene="cusS"
                     /locus_tag="ACHLCY_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP0370152.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5789 a         6543 c         6111 g         5569 t
ORIGIN      
        1 gcgaataatc ggcccaattt ttacaaacgc ccctggagag ataatatcca catgcgcaca
       61 atcctgacga tagatatcca gctccgcaat ccacggcgac caggcacgtt cgggactcat
      121 accttcctga agtgtgcgct cagcaacaaa taacgttgct tttccatcaa acggaacgct
      181 atgggcggtg gttaacaagc gcacagcatc agcgtagttg ccttcaatgg tggtaaacaa
      241 ctccgttgaa gtacttccct gctgtgccgc caggaaggcc tcgcgttcgc ggttaatctc
      301 cgccagcact tccgggtcca gaccattagc ctctttttcc tgccagtttt gcgtctctgg
      361 cggccaggta tccagcaaac caagaaatgc cacctgttcg ccacgggcac gcagtcgcgc
      421 cgcaataccc tgcgccagcg taccgccaag ggaatacccc agcaggtaat aagggccgtg
      481 cggttgttgc tcaagtaacg ttgccagatg cgcttcgcag acttcatcca ggtttgtcgc
      541 tgtctgcatg ggtccatgag ggcgcggcga ctgaatgcca ataatcgacc attgtggatc
      601 gagataacgc gagagcacgc taaactgcca ggcaaaaccg gacgccggat ggaaacaaaa
      661 cagcgtcggg ccattacctt cacgcaacgg cagaatggtt tcgaatccca tgcgccggga
      721 gctgtcctct tcaccatcaa taatcgttgc cagtttggcg acggttgacg cgaccatcac
      781 ctgccccggc gtcacctgac gagcaaactg tcgacttaac tgcgctgcta gtttcattgc
      841 cagtagcgaa tgaccgccca gcgcaaagaa atcagcatcg gcatcctgca cgtcacaacc
      901 cagcaacgac gcgaatgccg cggcgataat cgtttcactg cccgctttcg gtgcacgtcc
      961 cgacgcttgt gtcttcagtt caggcaacgg taaggctttg cgatccagct tgccgttggc
     1021 gctaagcggc aactgcggaa gttgcagcag aacgaccggc accatatgcg gcggcaatgt
     1081 ttcgcgaagc tgtgcctgta atgcgctggt atccaacggc agtcccgatt gcgacaccag
     1141 atagcccacc aactgacgcg catcaccacc ggtggctgcc gcctggttaa tcacacaggc
     1201 gtgggtaact gcctgttcga catccggcag cgcctgcatc acgcgatcga tttcgcccag
     1261 ttcgatacgc tgcccgcgaa tttttagctg atcgtcactg cgcccgaggt actccaccgc
     1321 gccgttatcc agccagcggg cgacgtctcc ggtacgatac atccgttcac ctggggcaaa
     1381 aggatcggca ataaaacggc tggcggtcag atcgggtcgt ccaagatacc cctgcgccag
     1441 ttggatgccg gtgagataga gatcacccgc cacacccggc ggcaccggat gcatcatcgc
     1501 atcaagaata cgcagacccg tattccatac cggataacca atcggcacac tgctgccgcg
     1561 cacctgtgcc agttcctcgc caaaagccgg ataccagctg acatctaccg ccgcttccgt
     1621 cgggccatac aggttatgca acggcgcgcc ggttaactgt tgccattcgc ggcataaatc
     1681 agccggtaag gcctcaccac tacagaaaac ctgtttcaac gtcgcgcaat tctggcgagc
     1741 ggtttgcggc gtcagcgatg caacaaatgc cgccagcatc gacggcacaa agtgcgtggt
     1801 cgttacgcca tattcggcaa agaattgctg catagcgagc gggtcacggt gcgcttccgg
     1861 ttcagccatc accagttttg cccctgcgat aaacggccag aaaaactccc acaccgagac
     1921 atcaaaactg cacggcgttt tttgggcaac gacatcttcg cctgtaagcg ggtagtgatt
     1981 ttgcatccat aacaggcggt taacgatagc cgtctgcccg accattaccc ctttcggcct
     2041 gccggtggag ccagaggtaa agatgatata agccgtgtgg tgcggttgtg aaagttgcag
     2101 cggcgcactg ccctgggggg taagcggggc gttatagcaa aggcttgtta aattgggaac
     2161 atcgctaaag cgcggcagtt gatcgtcggt ggtaattaac agcgacggac gcgcatcttc
     2221 cagcatcatt ttcaggcgat cgtccggata gccggtatcc agcggtagcc aggccgcgcc
     2281 agcttcaact attgcatgga gtgccagggt caaaaagacc gagcgcggta gtgccaccgc
     2341 cacgctgtcc cctggtttaa cgccgcgctc acgcagcaga ttcgccagcg ccaccacctg
     2401 ctcgcgcatt tcccgatagc tgaacaggta acgcgcatct gccagcgccg gagcatccgg
     2461 tgtttttgcc gcttgttctg ccaccagcgc gctcagcgtg gtttctggaa tctcaacctg
     2521 agtggcgttg atctgcgcca gctgcgcata ctcacctggc agcataatat cgacatcgcc
     2581 gcacaacagc gccggatccg cggcgaactg ggcaatcagc attttcaggc gttcagcatg
     2641 ctggattaac gttggctcat cgtaacgctg tttattggcg aggatctcaa tactcaaatc
     2701 accgtgttca tccgggaaca gggccagttc aaggtcatta accggaccgg ttgccagggt
     2761 atgggtttgc gtctgaacac caggaatatc cagttggtaa tcaaatacct tgatattgag
     2821 taccggaccg aacagcggtt cctctcctgc cgctcgcccg ctgtcacgga caatttgttc
     2881 ggcatcgtaa cgttgatgac gacgcatttt tttcagttgt gctgccagtc gggttgccag
     2941 ttccggcagc gtttcttgcg ccgcaatgtg aatacccaac ggtaaaacgt tgagcacggg
     3001 tccgctagcc gtcagcgccg ccgagcccag tcgacgcata aagataaatc cggcggcata
     3061 gtccatgcga ttgcacaatc gccccagcca caaggctgcc agcgcaaggg ctaaatcggt
     3121 acgctgcaca cctgaaagtt gcgtagccag ctggcggaat tccccgtcgg taaattccag
     3181 tttcaggcgc agaatatctg ccgaagcgct gcgccccgct aaaggtgccg gagaaagtga
     3241 cgcgggcggc ggcagttgac gacgctgttc tgcccagaat gccgcatcac gctgccaggc
     3301 ttcgctttcg cggtattgct ggtactcttc cactacatca gcgaaaggcg taaacggcga
     3361 agcaggcgtt ggttcgccac gcagccatgt gcagtaaata ttggcgatct ggcgagtaat
     3421 ggccgggaaa ctgaagccat cgaccagcaa atggtgataa cgctgatacc agtaccagcg
     3481 gttatccgca acatgtatca gctgatgaaa gaccagtggt ttaccgctat cgacgcgcaa
     3541 atcttgttgc aaatccgcca gcattaatgc ctgcgcagta ccgtgcggat caatattggt
     3601 tcgcaggtcg ataatttctg gcagttcgaa tatcagcgca tcatcgaccc actgccagac
     3661 ttcgccgtta tcttccgtaa aacgcatccg caacgtatct gcttgcgcta gtccgacaac
     3721 caccgcgcgg gccagtaatg gcgcatcaac atctccagtt aactcaacgt aatgcgccac
     3781 gctccaggcg gagggtaaat ccgacagttt ttctgccatc cagatgccgg gctgtgcggc
     3841 gaccaaaggt aaatgctggc tcattgtgcc tcctgcaact gggtgaaatt ggccggtgtc
     3901 agagtacgcc agtggacttc cagccactgc tggcaggacg cctgtgactg cggctgacac
     3961 acaatgtccc agcctgccgg taaggcgcat tgttgcggcc acagactgaa ttgcccctgc
     4021 gcattgcgca aaatgtaaaa cgctccctgt ggatcatcga agggattact gaatgccata
     4081 ttcaactcct gtcatggaaa agtggttgcc agaggtcgat tagcccctgc atcaagccac
     4141 cgcgccaaca aagcgcatca tgtccgccgt caacctgacg ccagaaaatg gattctttta
     4201 tggggtgtaa ttgcgcatac agcgcctgat tggcccgcat aatcatcggc tcgcgaatac
     4261 ccgcttccag cacaatgcgc agaccttcgg cgctaacttc accagcttta agcttttcaa
     4321 gtaacacgcc ctcttgctgc ccgccccgat gcggccacca gtacgatcct gactggctta
     4381 atacacagcc aaagcgttca ggccagtgca gtccggcata cagcgcggac agcccaccaa
     4441 aactctgccc ggcgaccacg gtgcgatcgg cgcgatcgct aaaaggggca atagctttca
     4501 ccaggggtaa taactcttgc tgtactgcca gccagaaatc cgcattacac ggcagttcgt
     4561 gggcgcggtg cgtggtgtcg atagcgtcga tcaacacata cacggcggga ggaagttgct
     4621 gacgatgggt cagcgaagtc agcacaggcc agacgggcat actttgcgcc caaaattcgc
     4681 catcgagcaa aactgccagc ggacgttctt ctgctgttgc atcgccggtg gtaaaaatcc
     4741 atacacgacg tgaatttttc aaccgttcac ttttccagat aatttctttg gctgctattt
     4801 ctggcgcttg cggacaatcc catcccggtt gcagaggcgc ttgcggcatt tcgagtgcag
     4861 aaacagcgtg ccctcgcccg cctttccagc tttgtggatt cagcggatcg gctattgcct
     4921 ggggtaatag ttttcgccag ccttcacgta attcgaggcg atcgggggat ggtgcagaaa
     4981 aaatgtcatc gcgttcggtg ggaataaagc agtagctgcc gcgccagttg gcattgagtt
     5041 gtgtcgtcca ctgccagaca tcagtacctg caattcgctg catcgactgg ggctggctgt
     5101 tctgatgatg atcggtcaca ccagtgatgt agatccatac gcgctttatc gtcgagtatt
     5161 cttcagaacc ttggggatca cgccaccaga aagtgacctc aaacatttcg tcattcagac
     5221 gctgccattc cgggccatgt ttcgactgcc accagctctc acttcctact tttaacgccg
     5281 tcacaccata accccatgtt tactgtgcaa tttttcattg attgcagaaa tatattgata
     5341 atattattga taactatttg catttgcaat agcgtaatgg cgcgccgtgg gaagcgcgga
     5401 catttattaa ccaactgcac tgcgtgtctt tcaggatcaa aggttttcgc ggtagcggga
     5461 tgcgtcgtgt tgaggacgac catgcccgac agttgcaatt cgtggcaaaa atgcaggaat
     5521 aaaacaatga acaagaagat tcattccctg gccttgttgg tcaatctggg gatttatggg
     5581 gtagcgcagg cacaagagcc gaccgatact cctgtttcac atgacgatac cattgtcgtt
     5641 accgccgccg agcagaactt acaggcgcct ggcgtttcga ccatcaccgc agatgaaatc
     5701 cgcaaaaacc ccgttgcccg cgatgtgtcg gagatcatcc gtaccatgcc aggcgttaac
     5761 ctgaccggta actccaccag tggtcagcgt gggaataacc gacagattga tattcgcggt
     5821 atgggtccgg aaaacacgct gattttgatt gacggcaagc cggtaagcag ccgtaactcg
     5881 gtgcgtcagg gctggcgtgg cgagcgcgat acccgtggtg atacctcctg ggtgccacct
     5941 gaaatgattg aacgtattga agttctgcgt ggtccggcag ctgcgcgtta tggcaacggc
     6001 gcggcgggcg gcgtggttaa catcattacc aaaaaaggca gcggtgagtg gcacggctcc
     6061 tgggacgctt atttcaatgc accagaacat aaagaggaag gtgccactaa acgcaccaac
     6121 ttcagcctga ccggtccgct gggcgacgaa ttcagcttcc gcttgtatgg caacctcgac
     6181 aaaacccagg ctgacgcgtg ggatatcaac cagggccatc agtccgcgcg tgccggaacg
     6241 tatgccacga cgttaccagc cgggcgcgaa ggggtgatca ataaagatat taacggcgtg
     6301 gtgcgctggg acttcgcacc attgcaatcg ctggaactgg aagcgggtta cagccgccag
     6361 ggtaacctgt atgcgggcga tacccagaac accaactctg acgcttacac tcgctcgaaa
     6421 tatggcgatg aaaccaaccg cctgtatcgc cagaactacg cgctgacctg gaacggtggc
     6481 tgggataacg gcgtgaccac cagcaactgg gtgcagtatg aacacacccg taactcgcgt
     6541 attccggaag gtctggcggg cggtaccgaa gggaaattta acgaaaaagc gacacaggat
     6601 ttcgtcgata tcgatctcga tgacgtgatg ctgcacagcg aagttaacct gccgattgat
     6661 ttcctcgtta accagacgct gacgctgggt acggagtgga atcagcaacg gatgaaggac
     6721 ttgagttcca atacccaggc gctgaccggg gcgaacaccg gcggtgctat tgatggtgtg
     6781 agtgccaccg accgtagccc gtattcaaaa gcagaaattt tctcgctgtt tgccgaaaac
     6841 aacatggagc tgactgacag caccatcgta acgccggggc tgcgtttcga tcatcacagt
     6901 attgtcggca ataactggag cccggcgctg aacatatcgc aaggtttagg cgatgacttc
     6961 acgctcaaaa tgggcattgc ccgtgcttat aaagcaccga gcctgtacca gactaacccg
     7021 aactacattc tctacagtaa aggtcagggc tgctatgcca gcgcgggcgg ctgctatctg
     7081 caaggtaacg atgacctgaa agcagaaacc agcatcaaca aagagattgg tctggagttc
     7141 aaacgcgacg gctggctggc gggcgtcacc tggttccgta acgattatcg caataagatt
     7201 gaagcgggct atgtggctgt agggcaaaac gcagtcggca ccgatctcta tcagtgggat
     7261 aacgtgccga aagcagtggt tgaaggtctg gaaggatcgt taaacgtacc agttagcgaa
     7321 acggtgatgt ggaccaataa catcacttat atgctgaaga gtgaaaacaa aaccacgggc
     7381 gaccgtttgt cgatcatccc ggagtacacg ttgaactcaa cgctgagctg gcaggcgcgg
     7441 gaagatttgt cgatgcaaac gaccttcacc tggtacggta agcagcagcc gaaaaaatac
     7501 aactacaaag gtcagccagc ggttggcccg gaaaccaaag aaatcagtcc ttacagcatt
     7561 gtgggtctga gcgcgacctg ggatgtgacg aagaatgtca gtctgaccgg cggcgtggac
     7621 aacctgttcg acaaacgttt gtggcgtgcg ggtaatgccc agaccacggg cgatctggca
     7681 ggggccaact atatcgcggg tgccggggcg tatacctata acgagccggg acgtacgtgg
     7741 tacatgagcg taaacactca cttctgatgc taacgtcaga ttgttgacaa agtgcgcgtc
     7801 gttcatgccg gatgcggcgt gaacgcctta tccggcctac aaaaatcttg ccaattcaat
     7861 atattgcagg accatgtagg cctgataagc gtagcgcatc aggcagttta gcgtttgtca
     7921 tcagtctcta atatggtcga catgaaaact acgcatacct ccctcccctt tgccggacat
     7981 acgctgcatt ttgttgagtt cgatccggcg aatttttgta agcaggattt actctggctg
     8041 ccgcattacg cgcaactgca acacgctgga cggaagcgca aagccgaaca tttagccggg
     8101 cggatcgctg caatttatgc gctgcgggaa tatggctata aatgtgtgcc cgcaatcggc
     8161 gagctacgcc aaccggtgtg gcctgcggag gtatacggca gtattagcca ctgcggggct
     8221 acggcattgg ccgtggtttc ccgtcaaccg attggcgttg atatcgaaga aattttttct
     8281 gcgcaaacag cgaccgaatt gacagacaac attattacac cagcagaaca cgagcgactc
     8341 gcagactgcg gtttagcctt ttctctggcg ctgacactgg cattttccgc caaagagagc
     8401 gcatttaagg caagtgagat ccaaactgat gcaggttttc tggactatca gataattagc
     8461 tggaataaac agcaggtcat cattcatcgt gagaatgaga tgtttgctgt gcactggcag
     8521 ataaaagaaa aaatagtcat aactctgtgc caacacgatt aattgacaac atctggtacg
     8581 attcgcccgc agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ttaccactct
     8641 gacctgacaa ccggatatgc ggggattgct ccccgcacaa cggctacttc ttcggttcgt
     8701 aagcgagaac agccttaaac tcaatattac gttcctttac ggtgaactca cacagcgagt
     8761 ctccgaccag aagcgtaaat cccagtaccg tcaaacacag cactattact gccacaaggg
     8821 catatttcgt cagcatgttt atattgcctc catgcgaaga agggactacc atccgagttg
     8881 ttgaggctcg gagtggcagc cccaggattg atagaaatat ctcctggggc ttcatctttc
     8941 cagacctcag agttaacctg aaaccggaaa gcttcaggca cccgccgtta tcttaccctt
     9001 cctcaacaaa atatctgttt atttatacag tgaatcctgt ttcggacttc tgtcggcaaa
     9061 actcatcatt gctcttaaaa cgaaatcgcc cgcagccatc actgaccgcg ggcgaaagtg
     9121 taaagcaggt gccttaccat cctgacctga caaccggata tgcggggatt gctccccgca
     9181 caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat tacgttcctt
     9241 tacggtgaac tcacacagcg aatccccgac cagaagcgta aatcccagca ccgttaaaca
     9301 cagcactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc ctccatgcga
     9361 agaagggact accatccaaa ttgttcaggt ttgggtaggt agccccaggg ttgatagaaa
     9421 tatcgtcctg ggacttcatc tttccagacc tcagagtaaa cctgaaaccg gaaagcttca
     9481 ggcacccgca gttatcttac ccttgcttag caaaatagct gtttatttat acagtaagtc
     9541 ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt tttttctgca
     9601 tgcctcaatc gtcaaaattc ttcgccacca gccagccgtg ccataacttg tcacattgca
     9661 aaagtagcca aattgcttca gtcaccatca ccaaaaaaag aaacattcca gaacttccgc
     9721 atctcgccca gccgctccga cttctatact gaatagaaaa cgccaactta agagaaacct
     9781 atgccattac ccgattttca tgtttctgaa ccttttaccc tcggtattga actggaaatg
     9841 caggtggtta atccgccggg ctatgactta agccaggact cttcaatgct gattgacgcg
     9901 gttaaaaatg agatcacggc cggagaggta aagcacgata tcaccgaaag tatgctggag
     9961 ctggcgacgg atgtttgccg tgatatcaac caggctgccg ggcagttttc agcgatgcag
    10021 aaagtcgtat tgcaggcagc cgcagaccat catctggaaa tttgcggcgg tggcacgcac
    10081 ccgtttcaga aatggcagcg tcaggaggta tgcgacaacg aacgctatca acgaacgctg
    10141 gaaaactttg gctatctcat ccagcaggcg accgtttttg gtcagcatgt ccatgttggc
    10201 tgcgccagtg gcgatgacgc catttatttg ctgcacggcc tgtcacggtt tgtgccgcac
    10261 ttcatcgccc tttccgccgc gtcgccgtat atgcagggaa cggatacgcg ttttgcctcc
    10321 tcacgaccga atattttttc cgcctttccc gataatggcc cgatgccgtg ggtcagtaac
    10381 tggcaacaat ttgaagccct gtttcgctgc ctgagttaca ccacgatgat cgacagcatt
    10441 aaagatctgc actgggatat tcgccccagt cctcattttg gcacggtgga ggttcgggtg
    10501 atggataccc cgttaaccct tagccacgcg gtaaatatgg cgggattaat tcaggccacc
    10561 gcccactggt tactgacgga acgcccgttt aaacatcagg agaaagatta cctgctgtat
    10621 aaattcaacc gtttccaggc ctgccgctat gggctggaag gcgtcattac cgatccgcac
    10681 actggcgatc gtcgaccgct aacggaagat accttgcgat tgctggaaaa aatcgcccct
    10741 tccgcacata aaatgggtgc atcgagcgcg attgaggccc tgcatcgcca ggtcgtcagc
    10801 ggtctgaatg aagcgcagct gatgcgcgat ttcgtcgccg atggtggctc gctgattggg
    10861 ctggtgaaaa agcattgtga gatctgggcc ggtgactaag tagggaattg ccttacagaa
    10921 gaatatccct gacaatggtg ttttaactta catttaacaa tgttatgaaa caccctttag
    10981 aaaccttgac taccgcagca ggcattttgc tgatggcttt cctctcttgc ctgctgctgc
    11041 ccgcccccgc actggggctg acgctggcac aaaaactggt gaccatgttc catctgatgg
    11101 atcttagcca gctttacact ttattgtttt gtctgtggtt tttagtgctg ggcgctattg
    11161 agtattttgt tctgcgcttt atctggcaac gctggttctc gctggcggat taaacgtcga
    11221 ttatcgccgt acttcgcggc atactttgct tattctcctt cgcctaaagg aacgtttatg
    11281 gataagcaat cactgcacga aacggcgaaa cgcctggccc ttgagttacc ctttgtcgag
    11341 ctttgctggc cttttggccc ggagttcgat gtttttaaaa ttggcggcaa aatttttatg
    11401 ctgtcgtcgg agctgcgcgg cgtccccttt atcaatctga agtccgatcc acaaaaatcc
    11461 ctgttaaatc agcagatata cccgagcatt aagccagggt atcacatgaa taaaaagcac
    11521 tggatttcgg tgtatcccgg cgaggaaatc tccgaagcgt tacttcgcga tctgatcaac
    11581 gattcgtgga atctggtggt tgatggtctg gctaaacgcg atcaaaaaag agtgcgtcca
    11641 ggctaaagcg gaaatctata gcgcattttt ctcgcttacc atttctcgtt gaaccttgta
    11701 atctgctggc acgcaaaatt actttcacat ggagtcttta tggatatcat ttctgtcgcc
    11761 ttaaagcgtc attccactaa ggcatttgat gccagtaaaa aacttacccc ggaacaggcc
    11821 gagcagatca aaactctcct gcaatacagc ccatccagca ccaactccca gccgtggcat
    11881 tttattgttg ccagcacgga agaaggtaaa gcgcgtgttg ccaaatccgc tgccggtaat
    11941 tatgtgttca acgaacgtaa aatacttgat gcctcgcacg tcgtggtgtt ctgtgcaaaa
    12001 accgcgatgg acgatgcctg gctgaagctg gttgttgacc aggaagatgc tgatggccgc
    12061 tttgccacgc cggaagcgaa agccgcgaac gataaaggtc gcaagttctt cgctgatatg
    12121 caccgtaaag atctgcatga tgatgcagag tggatggcaa aacaggttta tctcaacgtc
    12181 ggtaacttcc tgctcggcgt ggcggcgctg ggtctggacg cggtacccat cgaaggtttt
    12241 gacgccgcca tcctcgatgc agaatttggt ctgaaagaga aaggctacac cagtctggtg
    12301 gttgttccgg taggtcatca cagcgttgaa gattttaacg ctacgctgcc gaaatctcgt
    12361 ctgccgcaaa acatcacctt aaccgaagtg taattctctc ttgccgggca tctgcccggc
    12421 tatttcccct cagattctcc tgatttgcat aaccctgttt cagccgtcat cataggctgc
    12481 tgttgtataa aggagacgtt atgcaggatt taatatccca ggttgaagat ttagcgggta
    12541 ttgagatcga tcacaccacc tcgatggtga tgattttcgg tattattttt ctgaccgccg
    12601 tcgtggtgca tattattttg cattgggtgg tactgcggac cttcgaaaaa cgtgccatcg
    12661 ccagttcacg gctttggttg caaatcatta cccagaataa actcttccac cgtttagctt
    12721 ttaccctgca ggggattatc gtcaatattc aggcggtatt ctggctgcaa aaaggcaccg
    12781 aagcggcaga tattctgact acctgcgcgc agttgtggat catgatgtat gcgctgcttt
    12841 cagtcttctc gttgctggat gttattttga atctggcgca gaaattcccg gcggcatctc
    12901 agttaccgct gaaagggata tttcagggga ttaaactgat cggcgcaatc ctggtcggca
    12961 ttttgatgat ctcgctgctg attggtcagt cgccagcgat tctgatcagc ggtcttggtg
    13021 caatggctgc cgtgctgatg ttggttttta aagatccgat tcttggtctg gtggcaggta
    13081 ttcagctttc cgcgaacgat atgctgaaac tgggcgactg gctggagatg ccgaaatacg
    13141 gcgcggatgg cgcggtgatc gatattgggt taaccaccgt caaagtgcgt aactgggaca
    13201 ataccattac cactattccc acctggtctc tggtttctga ctcctttaaa aactggagcg
    13261 ggatgtcagc atctggcggg cgacgtatta agcgcagtat cagtattgat gtcaccagta
    13321 ttcgttttct tgatgaagac gaaatgcaac gtctgaataa agcgcacttg ttaaaacctt
    13381 atttaaccag ccgccatcag gaaattaatg agtggaatcg ccagcaaggt tctacggagt
    13441 cgatattaaa cctgcgccga atgaccaata ttggaacctt tcgtgcctat ctgaacgaat
    13501 atctgcgtaa ccatccgcgg attcgtaaag atatgacctt aatggtacgc caactggctc
    13561 caggtgataa cggtttaccg ctcgagatct acgcgtttac taacaccgtg gtgtggctgg
    13621 aatatgaaag cattcaggct gatatattcg atcacatatt tgcgattgtc gaagagtttg
    13681 gtctgcgact tcatcagtcg ccaaccggca atgatattcg ctctctggcg ggtgcattta
    13741 agcagtaatt aaaaaaaccg ctctcatcga atggatgaga gcggacatcg ttttgtcgga
    13801 tgtagcgtga atgccttatt tccgacgcag cgttttaaat gccacaaaca ggaatacaat
    13861 ccacaccggc agcaggatcg ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa
    13921 aatcatgccg aggaaggcaa tgcaaagata gttgccgaac ggatagagca gcgccttaaa
    13981 ctgtgtttca cgcccctgac gtcgcatcgc tgcacgaaaa cgcagatgcg ccagacagat
    14041 cataatccag ttcaacagca gcgttgctac caccagcgcc atcagcagac caaacgcttt
    14101 ttgcggcagc agatagttga ttaataccac cagcgaagtg atcgctccgg aaagcatcag
    14161 cgagttaatc ggcacaccgc gacggctgac gcgagtcaaa aacttcggcg cattaccctg
    14221 cacagaaagg ccaaacagca tgcggctgtt agagtaaacc ccgctgttat acactgacag
    14281 cgatgctacc agaatgacga agttcagcgc agaagctacc acgttgctgt cgagattatg
    14341 gaaaatcatc acaaacgggc tactgttgga tttcacttcc acccacggat agagcgccag
    14401 taaaaccacc agtgaaccga tgtaaaacag caggatgcga tacaccacct gatttaccgc
    14461 ttttggaatg cttttttccg gatcgcgcgc ttcagcggca gtaatcccaa tcagctccag
    14521 accgccgaag gagaacataa ttaccgccag cgacaaaatc agcccattcc agccggtggc
    14581 aaagaaacca ccgtagcgcc agaggttgtc gatactggct ttctcgccgc cgtgaccaga
    14641 aaacagcagc cacaggccaa agccgatcat accgatgatc gccagcactt taatcagcgc
    14701 aaaccagaac tcggtttcac catataagcg cacgttcacc aggttaacgg cgttgatgat
    14761 aataaagaag gcggcagccc aaatccacgt tggaacatcc gggaaccagt actgcatata
    14821 gatgcccgca gcggtcagct ctgccattcc caccagcacg aacattaccc agtagttcca
    14881 gccagagagg aagcccgcaa acggtcccca gtatttatag gcaaagtggg caaatgaacc
    14941 ggataccggc tcttcgacca ccatctcgcc aagctggcgc ataatcagga aagcgatgat
    15001 cccggcgacg ccgtagccca gcaatacagc cggacccgcc atctgaatcg ccgggccaat
    15061 gccaagaaac agaccagtac caattgcgcc acccagcgca atcagttgaa tatgacggtt
    15121 atgtaatccg cgatgaagcg tcggctcttg attcgacgca gtatcttccg atacggttga
    15181 cgcgtttttc acgcctttcc cctgtgtgtc ttttttgttg aggggcacct tttaacattt
    15241 agtgcccatc gtcgcaagac acaatccaca cggttaaacg gggtatcctg cttttatttc
    15301 cgtacccgat gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgac
    15361 agcaaaggtg cggtgatcat gccgccaatc atcggtgcgg caatccggct catcacctct
    15421 gaaccagccc ccgttcccca cagaatcggc agcagaccgg cgataatcac cgccaccgtc
    15481 atcgctttcg ggcgcacgcg caggaccgcg ccgtgatata acgcctcatc cagcttctgc
    15541 tcgctgaatg tttgtggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac
    15601 atcagcatca ccacgccaaa ttcggcggcg accccggcga gggcgataaa gccagtgccc
    15661 gtcgccacgg aaagatgaaa gcccatccac cacagcaacc agatgccgcc caccagcgca
    15721 aacggtacgc tgctgataat cagcaacgct tcgcccaccc gacggaacgc cagatacaac
    15781 agcacgaaga taatcatcaa cgtcatcggc accatgagtt taagcttatg gttggcgcgc
    15841 tccagtagct cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt
    15901 tcagctatcg ctttttgcaa atcgtgaacc accgacacca tgtcacgatc gcgggcatcg
    15961 atataaatcc agctcgtcgg gcgcgcattc tcggttttca gcattgacgg tccggtagag
    16021 actttaatgt cggccacgtc tgccagggtg atttgctgct tcatcggcgt caggatcggc
    16081 aactggcgca gtgcctgcgg actatcgcgc cagctttgcg gataacgcag attaattgga
    16141 taacgggcaa tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca
    16201 aacaactgca catccgccac cgtcataccg taacgtgcgg ctttttcacg gttaatctca
    16261 acgttgatat agcgcccacc ttccagacgc tcggctagag cagaagccac gcctggcacc
    16321 gttcgcgcta cttcttcaat ttgctcagcc atcgcgtcga tatccgccag cacagtgccg
    16381 gaaactttaa tgccgatggg gcttttaatg ccggttgaga gcatatcgat acggttacga
    16441 attggcggca cccacagatt cgccagcccc ggcagacgca cggtgttatc cagttcctca
    16501 atgattttgt ccattgtcat gcctggccgc cactgctcct gcggcttaag ctggatggtc
    16561 gtttctacca tctccagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca
    16621 aatacccgcg ccacttcagg tacgctcata attagcttgt cggttttttg cagcatactc
    16681 gccgcctctg ctgcggaaat ccccggcagc gtcgatggca tatacaacag gtcgccttca
    16741 ttgatctgcg gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc
    16801 gaaagcgctg ccaccaacag cgtggttttc ggccagtgca gtactttcag caacagcgga
    16861 tgataaacac gaatcaaaaa gcgattgagc gggttactgc tttccggcgg aattttgcca
    16921 cggatccagt aacccatcag gatagggatc actacgatcg ccagcagcgc cgcacccgcc
    16981 atcgcatacg ttttggtgaa cgccagcggg ccaaacagac gcccttcctg cccttccagg
    17041 gtgaagatcg ggataaacga caacgtgata atcagcagac tgataaatag cgccggaccc
    17101 acttcaacag atgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca
    17161 ggatgctggt gctgccactc ttccagccgt ttatgtgcat tttcgatcat gacgatagcg
    17221 gcatcgacca tcgccccgac ggcaatcgca atgccgccca acgacataat attggcattc
    17281 agtccctgga agtgcatgac aataaaagca atacacaacc ccagcagcaa cgaaataatc
    17341 gccaccagcg ccgagcgcac atgccagaga aacagcgcac agactaccgc caccacaata
    17401 aactcttcca gcaacttgcc gctgaggttg tcgatggcgc ggtcaatgag ctggctgcga
    17461 tcgtatgttg taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg
    17521 tccttcacgg cggcgatcac ttctcgggcg tttttgccgg atcgcaggat caccaccccg
    17581 cccgccactt cgccttcgcc gtttagttcg gcaatgcccc ggcgcatctc cgggccaatc
    17641 tggaccttcg caacatcgcg cagataaacg ggcacgccat tttcactggc ttttaaaacg
    17701 atgtgattaa agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat atattccgct
    17761 tccgccagtt cgatcgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt
    17821 acttcggcga gactgatgcc atactgcgcc aggcgctggg gatcgataac cacctgatac
    17881 tctttcacca caccgcccac cgacgccact tccgcaacgt cagggatggt tttcagctca
    17941 tatttgagaa accagtcctg caatgagcgt aaatcggcca gatcgtgctt accgctgcga
    18001 tccaccagtg catattcata gatccagcca acacccgtgg catctggtcc cagctcggcg
    18061 ctgactcccg caggcagctt accctgtacc tggttgaggt actccagcac ccgcgaacgc
    18121 gcccagtacg gatcggtgcc atcttcgaaa atgacataca cataagagtc gccaaactgt
    18181 gagaaaccgc gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa
    18241 gtcacctgat tttcaacgat ttgtggtgcc tgaccgggat agctggtttt aataataacc
    18301 tgcacatcgg agagatccgg cagcgcatcc actggcgtat taatgatggt ccaggtgccc
    18361 cagatgctca gaaacaacgc gcccatcagc accaaaaaac ggttcgccac cgagcgacga
    18421 ataatccatt caatcattgg ttattccctc aatgcgcatg ggtagcactt tcagagcgca
    18481 tccgctccag tgcgccagaa atattggctt cggaatcaat caggaacagg ccgctggaaa
    18541 ccaccttttc accttccgcc agaccagagc gtaatgcggt gacgccttgc gatgcctgga
    18601 aaacagcaac gcgtttcggt acaaagcgcc cgtcggcatc aacggtaatc acccgctgtt
    18661 cgctgccggt atcaatcagc gcttgtgacg gaatgagcag catcggttcg ctggcggtgt
    18721 tgagttgcaa ccaggcgttc atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca
    18781 gacgcagctg cagcgtgcgg gtcgcggcat ccacgccagg tagcagcgtc catttgcgga
    18841 tggtgagtgt tttatccggt cgcgccggaa cggtgagggt aaactgcgag gcatctttca
    18901 ccagccaggc gatggactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa
    18961 ttttcgcgac cacgttatct ttggcgatat tcattcccgc gcgcagatca aacgcggtga
    19021 tcacgccatc aatgggcgct ttgagcgtaa agcgagtctg gattttttgc gtggcgatca
    19081 ggcggcgaat atccgcctcc ggcattcccg ccagtcgcag ccgctcaaga atgccttcag
    19141 tctgggtcgc cgtaccgccg gtttcgcgca gcagtaaata ctcactctgc gcttccaccc
    19201 agtcaggaat ggtcaggtcg agaagcggtg tgcccttttg tactttatcg cccacggtaa
    19261 gcggatacac cttgtcgata aacccggcag cgcgagcctg cacaatggca tactgatact
    19321 cgttgtaact gacattcgcc gggaaactct gggcaaaagt cagcggcccg cgcgtgacgg
    19381 tagccgtttt cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag
    19441 aactctcttc atcggcatat ttcggcacca gatccatatc cataaacggc gatttacctg
    19501 gtttatcgaa ccgcgtattg gggtacatcg ggtcgtacca gaataagatt ttacgttctg
    19561 cggtcgacgt tttttctgcg ggtggttcag cctttgcaaa ccaggtaaaa cctgccgcag
    19621 aaataatacc gcccgcgatc atgctgccga taataagcgc gatttttttc atctcattaa
    19681 acctgggtta ctggctgact ttaatatcct gtaataaaga aaggttgccc tgctggacaa
    19741 aattaaacgc cactttgtcg ccagttttaa tttcactcat tttcgtctgc ggagtgatgg
    19801 taaagcgcat ggtcatctcc ggccagttca cggcagcaat cggatcgtga tggatggtga
    19861 tttttttgct ttccagatca atacccttta ccacgccagt ggcgctaata acctgcggtt
    19921 gtacttcgct catggtttca tgatgatgtt cgttagcctg ggcattaaag ccaataacgg
    19981 taaacagact gaacattgcg acttgcagtg cttttttcat ttattctctc ctggagttaa
    20041 aagtcatact ctaaataatt cgagttgcag gaaggcgaca agcgagtgaa tccccaggag
    20101 cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta
    20161 tgacgagtat attgctgtca accgccacct aaggcggtat acaaagaaat ttcgttaacc
    20221 tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg caaataaaga acgctcggca
    20281 tccagcactt ccagataact tactgcgccg tgctgatata atgcccgcgc ccgttgcaaa
    20341 gtaatttgca gcgacgccag ataacgctgc tgggcgctga tttgatcgtt caggctttga
    20401 cgtaatgcca gcgcatctgc cacttcttta aaggcgttct ggattttctg ttcataattc
    20461 actaccgact gctgctggcg aatttcggcg atatccagat tggcctggtt gcgtccggca
    20521 ttaaaaatgg ggatctcaat tttaggaata aaattccaca tcccgctgct ggcgttaaat
    20581 aatgacgaca gatcgctgct ggcggttgat attccgctgg tcagccttat agatgggaaa
    20641 aatgccgcgc gtgcggcccc aatattggca ttagccgcca ttaacgcgtg ttcagcttcc
    20701 ataatatccg ggcgctgcaa taagatttgc gacgacaatc ccgccggtaa tttaacgctt
    20761 tgcaggctgt cgctgtttac tgtctgcgct tgcggcagct tgccgtagct tcccaataac
    20821 agttgcaatg cattattcgc ctgcgccagt tccccctgac gtttagcgat gtcgctgcgg
    20881 gtactttcaa tcaccccgcg agcctgttcc agcgccagaa cattactgct acctgtcaac
    20941 agttgttttt cgacaaacgc atatgactgc tgataattac gcagcgtttc ttcggctatt
    21001 tgcaattgcg catacgctag ctgctgattg aaataacttt gcgcgacatt agaaaccagc
    21061 aaaatatgca ccgcgcgctg ggcttcctca gtggctaaat aattttggcg ctcggcttcg
    21121 ctcatgttct ttaagcgacc gaaaaaatcg agatcaaagc tggcgttaag gccggtcgaa
    21181 aactcccgtg tcgtggcggt attcccttta agattaccgc tccagctgcc gctgccctcg
    21241 ccattgagct gtgggtagcg gtcggcatcg gtcagacgat attgcgcccg cgcttcctgc
    21301 actttcagcg tcgccatgcg caaatcccgg ttattcacca gcgcctcgct aatcagcgtc
    21361 tttacctgat tatcaacaaa aaaggtgcgc cagcccacgt tctggtagtt atctgctgcg
    21421 ttaaccaagc cgttctggct gagtgagaac tgctgcggca cgggcattgc cggacgctga
    21481 taatccggtg ccagtgaaca accggttagc gcaagggcca cacaaaatgg cagaagttta
    21541 caaggagaca taggctcata atttctggtg attttatacc gccaacttta ctcgccaggc
    21601 tctgattttc cggtgacagg aaaatgacaa aattgtcatt ttgccaataa gcgattgcca
    21661 tctgatcccg ctactctaga atagcccggg caacatgcgg aggaaaaatg aaactgttga
    21721 ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa agggttaacc gaagccggtt
    21781 ttgtggtcga tttggccgac aacgggctga atggctacca tctggcgatg accggtgatt
    21841 atgatctgat aatcctcgat attatgctgc cggacgtgaa cggctgggat atcgtgcgca
    21901 tgttgcgttc cgccaataaa gggatgccga ttctgttgct taccgcgctt ggcaccattg
    21961 aacatcgcgt caaggggctg gagttggggg cagatgacta ccaggtgaaa ccattcgctt
    22021 ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg cggggcggcg gtgattatcg
    22081 aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt cagccgcaaa gtcacccgca
    22141 gcggcacgcg catcactctg accagtaaag agtttactct gctggagttt ttcctccgcc
    22201 atcagggcga agtgctgccc cgctcgctta tcgcctcgca ggtgtgggac atgaatttcg
    22261 acagcgacac taacgccatt gatgtagcgg tgaagcggct gcgcggcaaa atcgacaacg
    22321 actttgagcc aaagctaatt cagaccgtgc gcggcgtggg ttacatgctt gaggtgccgg
    22381 atggtcagta agccatttca gcgcccgttt tcgctggcaa cccgcctgac cttttttatc
    22441 agcctggcca ccatcgcagc gtttttcgcc tttgcatgga tcatgatcca ctcagtaaaa
    22501 gtgcattttg ccgagcagga tattaatgat ttaaaagaaa ttagcgccac gcttgaacgg
    22561 gtacttaatc atcctgacga gacgcaagcc cgacgcttaa tgacgctgga agatatcgtc
    22621 agtggttatt ccaacgtgtt gatctccctg gcagatagcc acggcaaaac ggtgtatcac
    22681 tcccccggtg cgcctgatat ccgcgagttt gcacgtgatg ccatacccga taaagacgcg
    22741 cggggtggcg aggtgtatct cctttccggc ccgacgatta tgatgccagg ccacggtcac
    22801 gggcatatgg aacacagcaa ctggcggatg attaacttgt cggttggccc gttggtggac
    22861 ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg attttcatct tcattacata
    22921 aatgatttga tgaataaact tattatgacc gcatcggtaa tcagcatcct gatcgtcttt
    22981 atcgtactgt tggcggtaca taaaggtcac gcgccgatcc gcagcgtcag ccgtcaaatc
    23041 cagaatatca cctcgaaaga tctcgacgtt cgccttgacc cgcagaccgt gcccattgag
    23101 ctggaacagc tggtactgtc gttcaaccat atgatcgagc gtattgagga tgtctttacc
    23161 cgccagtcca atttctcagc ggatatcgct cacgaaattc gcacgccgat taccaatctc
    23221 atcacgcaaa cggaaatcgc cctcagccag tctcgcagcc agaaggagct ggaagatgtg
    23281 ctctactcta atctcgaaga gctgacgcga atggcgaaaa tggtcagcga tatgctgttt
    23341 ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga aaatgctcaa cctggcggat
    23401 gaagtcggca aagtgttcga ttttttcgag gcgttagcgg aagatcgcgg cgtggagttg
    23461 cgatttgttg gcgacgaatg tcaggttgcg ggcgatccgc tgatgctgcg tcgggcgtta
    23521 agcaacctgc tttctaatgc cctgcgttat acgccgcccg gagaggcaat tgtagtgcgc
    23581 tgccagacgg tcgatcacca ggtgcaagtt accgtcgaaa accccggtac gcccattgcg
    23641 cccgagcact taccgcgatt gtttgaccgt ttctaccgcg ttgacccttc ccgccagcga
    23701 aaaggcgaag gtagcggcat tgggctggcg atagtgaaat cgattgttgt cgcgcataaa
    23761 ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt ttgtgattat gctgccggag
    23821 agagagtgat tattaagttc aaattcaggg cgtggtgcat cacctcctgg tattgttgat
    23881 caacaaatta gagaaaaact tattaaacat atgaaatatc gaaggctttt atttttcaaa
    23941 gtttcacgag atgtttgtat atgaaactta gttaaatctg tgtttcttcc aattgtagat
    24001 cacaatgccc tt
//
LOCUS       DAZGTR010000068        23599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGTR010000068 DAZGTR010000000
VERSION     DAZGTR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.068"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(463..1260)
                     /gene="ybcM"
                     /locus_tag="ACHLCY_002937"
     CDS             complement(463..1260)
                     /gene="ybcM"
                     /locus_tag="ACHLCY_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0370153.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1270..1821)
                     /gene="ybcL"
                     /locus_tag="ACHLCY_002938"
     CDS             complement(1270..1821)
                     /gene="ybcL"
                     /locus_tag="ACHLCY_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFP0370154.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEMKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1990..2322)
                     /gene="emrE"
                     /locus_tag="ACHLCY_002939"
     CDS             complement(1990..2322)
                     /gene="emrE"
                     /locus_tag="ACHLCY_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP0370155.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2656..2970)
                     /gene="fliE"
                     /locus_tag="ACHLCY_002940"
     CDS             complement(2656..2970)
                     /gene="fliE"
                     /locus_tag="ACHLCY_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP0370156.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3184..4842
                     /gene="fliF"
                     /locus_tag="ACHLCY_002941"
     CDS             3184..4842
                     /gene="fliF"
                     /locus_tag="ACHLCY_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP0370157.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRVSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFREQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PVPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAHEEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4835..5830
                     /gene="fliG"
                     /locus_tag="ACHLCY_002942"
     CDS             4835..5830
                     /gene="fliG"
                     /locus_tag="ACHLCY_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP0370158.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5823..6509
                     /gene="fliH"
                     /locus_tag="ACHLCY_002943"
     CDS             5823..6509
                     /gene="fliH"
                     /locus_tag="ACHLCY_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP0370159.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6509..7882
                     /gene="fliI"
                     /locus_tag="ACHLCY_002944"
     CDS             6509..7882
                     /gene="fliI"
                     /locus_tag="ACHLCY_002944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP0370160.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTGS"
     gene            7901..8344
                     /gene="fliJ"
                     /locus_tag="ACHLCY_002945"
     CDS             7901..8344
                     /gene="fliJ"
                     /locus_tag="ACHLCY_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP0370161.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8341..9468
                     /gene="fliK"
                     /locus_tag="ACHLCY_002946"
     CDS             8341..9468
                     /gene="fliK"
                     /locus_tag="ACHLCY_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0370162.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISSESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9573..10037
                     /gene="fliL"
                     /locus_tag="ACHLCY_002947"
     CDS             9573..10037
                     /gene="fliL"
                     /locus_tag="ACHLCY_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP0370163.1"
                     /translation="MTDYAISKKSKRSLWIQILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            10042..11046
                     /gene="fliM"
                     /locus_tag="ACHLCY_002948"
     CDS             10042..11046
                     /gene="fliM"
                     /locus_tag="ACHLCY_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP0370164.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11043..11456
                     /gene="fliN"
                     /locus_tag="ACHLCY_002949"
     CDS             11043..11456
                     /gene="fliN"
                     /locus_tag="ACHLCY_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP0370165.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11459..11824
                     /gene="fliO"
                     /locus_tag="ACHLCY_002950"
     CDS             11459..11824
                     /gene="fliO"
                     /locus_tag="ACHLCY_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP0370166.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11824..12561
                     /gene="fliP"
                     /locus_tag="ACHLCY_002951"
     CDS             11824..12561
                     /gene="fliP"
                     /locus_tag="ACHLCY_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP0370167.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12571..12840
                     /gene="fliQ"
                     /locus_tag="ACHLCY_002952"
     CDS             12571..12840
                     /gene="fliQ"
                     /locus_tag="ACHLCY_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP0370168.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12848..13633
                     /gene="fliR"
                     /locus_tag="ACHLCY_002953"
     CDS             12848..13633
                     /gene="fliR"
                     /locus_tag="ACHLCY_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0370169.1"
                     /translation="MLQVTSEQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            13923..14546
                     /gene="rcsA"
                     /locus_tag="ACHLCY_002954"
     CDS             13923..14546
                     /gene="rcsA"
                     /locus_tag="ACHLCY_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP0370170.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14590..14778)
                     /gene="dsrB"
                     /locus_tag="ACHLCY_002955"
     CDS             complement(14590..14778)
                     /gene="dsrB"
                     /locus_tag="ACHLCY_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP0370171.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14941..15168
                     /gene="yodD"
                     /locus_tag="ACHLCY_002956"
     CDS             14941..15168
                     /gene="yodD"
                     /locus_tag="ACHLCY_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP0370172.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15466..16281
                     /gene="yedP"
                     /locus_tag="ACHLCY_002957"
     CDS             15466..16281
                     /gene="yedP"
                     /locus_tag="ACHLCY_002957"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP0370173.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16278..17972)
                     /gene="dgcQ"
                     /locus_tag="ACHLCY_002958"
     CDS             complement(16278..17972)
                     /gene="dgcQ"
                     /locus_tag="ACHLCY_002958"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP0370174.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18143..18325)
                     /gene="yodC"
                     /locus_tag="ACHLCY_002959"
     CDS             complement(18143..18325)
                     /gene="yodC"
                     /locus_tag="ACHLCY_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP0370175.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVESRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18404..19321)
                     /gene="yedI"
                     /locus_tag="ACHLCY_002960"
     CDS             complement(18404..19321)
                     /gene="yedI"
                     /locus_tag="ACHLCY_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP0370176.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19494..20414
                     /gene="yedA"
                     /locus_tag="ACHLCY_002961"
     CDS             19494..20414
                     /gene="yedA"
                     /locus_tag="ACHLCY_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP0370177.1"
                     /translation="MRFGQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(20403..20873)
                     /gene="vsr"
                     /locus_tag="ACHLCY_002962"
     CDS             complement(20403..20873)
                     /gene="vsr"
                     /locus_tag="ACHLCY_002962"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP0370178.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20854..22272)
                     /gene="dcm"
                     /locus_tag="ACHLCY_002963"
     CDS             complement(20854..22272)
                     /gene="dcm"
                     /locus_tag="ACHLCY_002963"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP0370179.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22339..23034)
                     /gene="yedJ"
                     /locus_tag="ACHLCY_002964"
     CDS             complement(22339..23034)
                     /gene="yedJ"
                     /locus_tag="ACHLCY_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP0370180.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(23074..23355)
                     /gene="drpB"
                     /locus_tag="ACHLCY_002965"
     CDS             complement(23074..23355)
                     /gene="drpB"
                     /locus_tag="ACHLCY_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP0370181.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5849 a         6137 c         5913 g         5700 t
ORIGIN      
        1 aaataatctc attgattgga tgaatattta tagtgcagtg acatcatttt taaataatag
       61 ttcaaataaa agggctcacg atgaaaaaat taacagtggc aatttctgct gtagttgcat
      121 cttaacgtat aaaccagatt taataacaac gaactagatt taataacaac gatatccgta
      181 cctatccgga tatctaatgt cataagcatc cctttaatta tcccctcaac ctcttgtaac
      241 tttcccactc ttgaactcca catagtccaa acgccggatg caaaaaaatc acacgtgcat
      301 ttctgaaatt gtgatggagg acgatagtat caattatatg tgcttataaa cgtccccgat
      361 tagcgcagaa gtcaccggat tgttcaggct ccaataggtt gagtatgccg tatttatttt
      421 ccaaaataaa aatcgatgct gttgctcctt cataaaatac ttttactctc tggacgcttt
      481 tcttagctct cttggtgtta tcttaaaacg attatggaac ctttcagtaa aacgagaagg
      541 acacttgtaa ccattttccc tggcaatctc gctgatgggt tttactgtcg tttgtatagc
      601 agacaacgca ttatttaacc tcacgtcgtc caatatactt tgaaaactta ctccctcgct
      661 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
      721 tgtccatttt gttccaggtt cggatagcat taggttataa caacgactta ttaatgattg
      781 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      841 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      901 ctcccaacaa tatattaact cgggattctg aggtaagcag aaacctgtcg tgttacgaat
      961 ttgatcggtt atcgcataaa gattttggaa cttttcaatt agatcaatgg gataataaag
     1021 catttctgca agataaagac ctgcttctgg gtaattcgca atataaaatt catatccaca
     1081 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
     1141 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaaccc tgtgaatctc
     1201 atgatgttta tgcatccgta tttcttcaat ctttaggtta gtcctatctt ttgccagcat
     1261 actcttatcc tactttacct cataaacggg tattatctca gcggttgcaa ttttattggc
     1321 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
     1381 ttttagagcc catattttaa actggtaatg atggggttta tctcctttgg gaggacatgc
     1441 tccaccaaat cccgcatagc caaaatcatt tcggccttgg acagcaccag tcggaagttt
     1501 tgtaccatca cgtcttcctg catcagcggg caaatatgtt accgttgctg gaatattagc
     1561 aacagtccag tgccaccatc cgctgcctgt aggcgcatct ggatcataca cagttacggc
     1621 aaagcttttg gtgccttctg gagcaccaga ccaggttaat gagggcgatg tattaccccc
     1681 ctcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg ttttcatttc
     1741 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1801 tgaaacgata agttttttca taaaaacctc tttgttgtga cctatcgtta ttttatttga
     1861 tgtgcattga tctcattatg caaaaaggcg caatgatcgc gcaaatgcaa tcataattat
     1921 aaagttgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggat
     1981 gattattttt taatgcggtg cgcttcgtga caacaagtta atgactaaca caccggcaca
     2041 aatcaacatc atgcctatga tggctggcaa gtccagtcgt tggccaaaaa atccccagga
     2101 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     2161 tatataagcc agtgtctgag ctaataacca aaatgatgca caatagcaaa taattgtgcc
     2221 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     2281 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     2341 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctggtgtat
     2401 gtgtatccag ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     2461 gaaccccagg ctaacaaaag agttcactct tcctatgagc aaagcgaaga caaaacatgt
     2521 tatcgcgttt acggatcgtg gatggcagaa aactttcagg atcaggtatc cattttaaac
     2581 cagaaatagg gtgagtttgc cccatccatg ccccacgcgg gcagatggga tgtaattacc
     2641 aacaggttac aaaacctaca cctgcatgga catcacttcc tgatacgccg ccaccagctt
     2701 attacgcacc tgaatcccca tttgcataga aactgaggct ttttgcatat cggtcatcac
     2761 atcgtttaac gccacgccgg gttcaccgag ggtgaatttt tctgcctgcg tgcgggcggc
     2821 tgtttgtgta tcgcttatgc gatcgagcgc ggcgtgcagc tgcccggcaa aactaatggt
     2881 cggttgtggc agtgattcct gtgcacgcgc actcatcgcc gtagcctgta actggctgat
     2941 aaccccttca atcccctgta tcgctgacat tttcgtctcc cggataattt ctggtagcaa
     3001 agcctaccag taagtcaata agacaaaggc gctaaatagc aacaaaaaaa cgggtttatt
     3061 ggcggataga aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtttgctga
     3121 cccgggagtg agtctgttcc actttgccaa taacgccgtc cataatcagc cacgaggtgc
     3181 gcgatgaatg cgactgcagc ccagacaaaa tctcttgagt ggcttaatcg cctgcgtgcg
     3241 aatccgaaaa ttccattgat tgttgccggt tccgcggcag tggcggtcat ggtcgcactg
     3301 atcctgtggg cgaaagcccc cgactaccgc acattattca gcaatctttc cgatcaggat
     3361 ggtggcgcaa ttgtcagcca actgacgcaa atgaatattc cttaccgcgt cagcgaagcc
     3421 agcggcgcta ttgaagttcc ggcagataaa gttcacgagc tgcgtctgcg cctggcgcaa
     3481 caaggtttgc ccaaaggcgg cgcggtcggt ttcgaactac tcgatcagga aaagtttggt
     3541 atcagccagt tcagagaaca ggtgaattat cagcgggcgc tggaaggcga gctttctcgt
     3601 accatcgaaa ctatcggccc ggtaaaaggg gcgcgcgtac atctggcaat gccgaaaccg
     3661 tctttattcg tccgtgaaca aaaatcccct tctgcatcgg tgacggtaaa tctgttaccc
     3721 ggccgcgcac tcgatgaagg gcaaattagc gccattgtac atctggtttc cagcgcggtt
     3781 gctggtctgc cgccggggaa cgtcacgctg gtggatcagg gtggacatct gttaacccag
     3841 tccaatacca gcgggcgcga tcttaatgac gctcagttga aatatgccag cgatgtcgaa
     3901 ggccggattc agcggcgtat tgaagcgatc ctgtcgccaa ttgttggtaa cggtaatatt
     3961 cacgcccagg tcacggcgca actggacttc gccagtaaag aacaaacgga agaacagtat
     4021 cgccctaacg gtgatgaatc tcaggcggcg cttcgttcac gccagcttaa tgagagcgag
     4081 caaagcggtt ccggttatcc gggcggcgta ccgggggcgt tgtcgaatca accggcacct
     4141 gcgaataacg cgccaatcag cacgcctccg gcaaatcaaa ataaccgcca gcagcaggcg
     4201 agcaccacca gcaatagcgg gccgcgtagc acacagcgga atgaaaccag taactacgaa
     4261 gtcgatcgca ccattcgtca taccaaaatg aacgtgggcg atgtgcaacg tctgtcagtc
     4321 gcggttgtgg tgaattacaa aaccttgcca gatggcaaac cggtgcctct cagcaacgaa
     4381 cagatgaagc aaattgaaga tctgacccgc gaggcgatgg gcttttctga aaaacgcggt
     4441 gactcgctca atgtcgtcaa ctcgccgttc aatagcagtg acgaaagcgg cggagaactg
     4501 ccgttctggc aacagcaagc gtttatcgat cagttacttg ctgccggtcg ctggttgctg
     4561 gtactgctgg tggcgtggct gctgtggcgt aaagcggtac gcccgcagct aacacgtcgc
     4621 gctgaggcga tgaaagctgt acagcaacag gcgcaggccc acgaggaagt ggaagatgcg
     4681 gtggaagtcc gcctgagcaa agacgaacaa ctacaacaac ggcgcgctaa ccaacgtctg
     4741 ggggcagaag tcatgagcca gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg
     4801 gcgctggtca ttcgccagtg gataaataac gatcatgagt aacctgacag gcaccgataa
     4861 aagcgtcatc ctgctgatga ccattggcga agaccgggcg gcagaggtgt tcaagcacct
     4921 ctcccagcgc gaagtgcaaa ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa
     4981 caagcagcta accgatgtgc tggcggagtt tgagcaagaa gctgaacagt ttgccgcact
     5041 gaatatcaac gccaacgatt atctgcgttc ggtattggtc aaagctctgg gtgaggaacg
     5101 tgccgccagc ctgctggaag atattctcga aactcgcgat accgccagcg gtattgaaac
     5161 gctcaacttt atggagccgc agagcgccgc cgatctgatt cgcgatgagc atccgcaaat
     5221 tatcgccacc attctggtgc atctgaagcg cgcccaggcc gccgatattc tggcgttgtt
     5281 cgatgaacgt ctgcgccacg acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc
     5341 agccgcgctg gcggagctga ccgaagtact gaatggcttg ctcgacggtc agaatctcaa
     5401 gcgcagcaaa atgggcggcg tgagaacggc agccgaaatt atcaacctga tgaaaactca
     5461 gcaggaagaa gccgttatta ccgccgtgcg cgaattcgac ggcgagctgg cgcagaaaat
     5521 catcgacgag atgttcctgt tcgagaatct ggtggatgtc gacgatcgca gcattcagcg
     5581 tctgttgcag gaagtagatt ccgaatcgct gttgatcgcg ctgaaagggg ccgagcagcc
     5641 actgcgcgag aaattcctgc gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga
     5701 tctcgccaac cgtggtccgg tgcgtctgtc gcaggtggaa aacgaacaga aagcgattct
     5761 gctgattgtg cgccgccttg ccgaaactgg cgagatggta attggcagcg gcgaggatac
     5821 ctatgtctga taatctgccg tggaaaacct ggacgccgga cgatctcgcg ccaccacagg
     5881 cagagtttgt gcccatggtc gagccggaag aaaccatcat tgaagaggcc gaacccagcc
     5941 ttgagcagca actggcgcaa ctgcaaatgc aggcccatga gcaaggttat caggcgggga
     6001 ttgccgaagg tcgccagcaa ggtcatgagc agggctatca ggaaggactg gcccaggggc
     6061 tggagcaagg tctggcagag gcgaagtctc aacaagcgcc aattcatgcc cggatgcagc
     6121 aactggtcag cgaatttcaa actacccttg atgcacttga tagtgtgatt gcttcgcgcc
     6181 tgatgcagat ggcgctggag gcggcacgtc aggtcatcgg tcagacgcca acagtggata
     6241 actcggcact gatcaaacag atccaacagt tgttgcagca agaaccgtta ttcagcggta
     6301 aaccacagct gcgcgtgcac ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa
     6361 ccttaagttt gcatggctgg cgcttgcggg gcgatcccac actccatcct ggcggctgta
     6421 aagtctccgc cgatgaaggc gatctcgacg ccagtgtcgc cactcgctgg caagaactct
     6481 gccgtctggc agcaccagga gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac
     6541 gctggataac ttcgaagcca aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt
     6601 aacccgcgct accgggctgg tgctggaagc caccggatta caattgccgc tcggcgcaac
     6661 ctgtgtcatt gagcgtcaga acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg
     6721 ctttaacggt caacggctgt ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg
     6781 cgcgcgggtt tatgccaaaa acatttcggc tgaaggactg caaagcggca agcagttgcc
     6841 gctcggtccg gcgttattag gtcgcgttct ggacggcagc ggtaaaccgc tcgatggcct
     6901 cccctccccc gatacgacgg aaaccggtgc gctgattacc ccaccattta acccattgca
     6961 acgtacaccg attgaacatg tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac
     7021 cgtcgggcgc gggcagcgta tggggctgtt tgccgggtcc ggcgttggta aaagtgtgct
     7081 gctggggatg atggcccgtt acacccgcgc cgatgtcatt gtcgttggtt taattggtga
     7141 acgcggacgc gaagtaaaag attttattga gaatatcctc ggtgccgaag gacgtgcacg
     7201 ctcagtagtg attgccgctc cggcggatgt ttctccgctc ctgcgaatgc agggtgccgc
     7261 ctatgccacg cgcattgccg aagattttcg cgatcgtggt cagcatgtgt tgctgattat
     7321 ggactccctc acccgctatg cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc
     7381 acccgccacc aaaggttatc ccccgtcggt gtttgccaaa ttaccggcac tggtcgagcg
     7441 tgccggaaat ggcattagcg gcggcggctc gattaccgcg ttttataccg tgctcaccga
     7501 gggcgatgac cagcaggacc ccattgccga ctctgcgcgg gcaatcctcg acggtcacat
     7561 tgtgctgtct cgccgactgg cggaagccgg gcactatccg gctatcgata ttgaagcgtc
     7621 gatcagccgc gcaatgacgg cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt
     7681 caaacagctg ttgtcgagtt ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc
     7741 caaaggcagc gatccgatgc tcgacaaagc catcgccctg tggccgcagc tggagggcta
     7801 tttgcaacaa ggcatttttg aacgcgcgga ctgggaagcg tctctccagg ggctggagcg
     7861 tattttcccg acagggtcat aacccaggag ataacggcag atggcagaac atggcgcgct
     7921 ggcgaccctg aaagatctgg cagaaaaaga ggtagaggat gccgcgcgcc tgctgggtga
     7981 aatgcgtcgc gggtgtcagc aggcggaaga acagctcaaa atgctgatcg attatcagaa
     8041 tgaatatcgc aataacctca acagcgatat gagtgccggg atgaccagca accgctggat
     8101 caactatcag cagtttatcc agacgctgga aaaagccatt actcagcatc gccagcaact
     8161 taatcagtgg acgcagaaag ttgacattgc cctgaacagc tggcgagaaa agaaacaacg
     8221 tttgcaggcc tggcagacac tgcaggaacg gcaatccacg gcggcactgc ttgcagaaaa
     8281 ccgcctcgat cagaaaaaga tggatgagtt cgcccagcgc gccgccatga ggaaacctga
     8341 atgattcgct tagcgccctt gattaccgcc gacgttgaca ccaccacatt gcctggcggc
     8401 aaagccagcg atgctgcaca agattttctc gcgttgttga gcgaagcatt agcaggcgag
     8461 acaactaccg acaaagcggc cccccagttg ctggtggcaa cagataagcc cacgacaaaa
     8521 ggcgagccgc tggtcagcga gattcttgcc gatgcgcaac aagcggattt actgatccct
     8581 gtggatgaaa caccgcctgt catcaacgac gaacaatcca catcaacacc attaaccacc
     8641 gctcaaacga tgacgttggc tgcggtggct ggcaacaata cggcaaaaga cgaaaaagcg
     8701 gatgatctga atgaagacgt caccgcaagc ctgagcgccc tttttgcgat gttgccgggt
     8761 tttgacaata cgcccaaagt gactgatgcg ccgtcaaccg tgttaccgac agagaaacca
     8821 acgctcttta caaaactgac ttctgcgcaa ctcacaacag cacagcctga tgatgccccc
     8881 ggcacgccag ctcagccatt aacaccgctg gtagcagaag cccagagtaa agcggaagtc
     8941 atcagcacac cgtcaccggt gaccgctgcc gccagcccgc taattactcc acaccagaca
     9001 cagccactgc ccaccgtcgc cgcgcctgtg ttgagtgcac cgctgggttc tcacgaatgg
     9061 caacaatcat taagccagca tatttcgctg ttcacccgcc aggggcaaca aagtgcagag
     9121 ttgcgtctgc acccgcagga tttaggtgaa gtgcaaatct ccctcaaagt ggatgataac
     9181 caggcacaaa tccagatggt ttcaccgcat cagcatgtac gcgccgccct ggaagcagcg
     9241 ctgccggtac tgcgtacgca gctggccgaa agtggcattc agttagggca aagcaacatc
     9301 agtagcgaaa gctttagtgg tcagcagcag gccgcttccc aacaacagca aagccaacgc
     9361 acagtaaacc atgaacctct ggcgggggaa gaagacgata cgcttccggt tcccgtctct
     9421 ttacaagggc gcgtaacagg caacagcggc gttgatattt tcgcctaacg tcagaggtag
     9481 caccgtaatc cgcgtctttt ccccgctttg ttgcgctcaa gacgcaggat aattagccga
     9541 taagcagtag cgacacagga agaccgcaac acatgactga ttacgcgata agcaagaaaa
     9601 gcaagcgatc gctttggatc cagattctgg tattcattac cctcgcggcc tgtgccagcg
     9661 caggttacag ctactggcat tcgcatcagg ttgccgctga agacaaagcg cagcaacgcg
     9721 tcgtgccctc cccggtcttc tatgcgctgg atacctttac ggtcaatctg ggcgatgcag
     9781 atcgcgtact ttatatcggc ataaccctgc gcctgaaaga tgaagctacc cgctcgcggc
     9841 tgagtgagta tttgccggaa gtccgtagcc gtttgctgtt actgttttcg cgtcaggatg
     9901 ctgccgtact ggcgacagaa gaaggcaaga aaaacctgat tgccgagatt aaaaccacgc
     9961 tttccacccc gcttgttgcc gggcaaccga aacaggatgt caccgacgtg ctgtataccg
    10021 cttttattct gcgataacga catgggcgat agtattcttt ctcaagctga aattgatgcg
    10081 ctgttgaatg gtgacagcga agtcaaagac gaaccgacag ccagtgttag cggcgaaagt
    10141 gacattcgtc cgtacgatcc gaatacccaa cgacgggttg tgcgcgaacg tttgcaggcg
    10201 ctggaaatca ttaatgagcg ctttgcccgc cattttcgta tggggctgtt caacctgctg
    10261 cgtcgtagcc cggatataac cgtcggggcc atccgcattc agccgtacca tgaatttgcc
    10321 cgcaacctgc cggtgccgac caacctgaac cttatccatc tgaaaccgct gcgcggcaca
    10381 gggctggtgg tgttttcacc gagtctggtg tttatcgccg tggataacct gtttggcggc
    10441 gatggacgct tcccgaccaa agtggaaggt cgcgagttta cccataccga acagcgcgtc
    10501 atcaaccgca tgttaaagct ggcgcttgaa ggctatagcg acgcctggaa ggcgattaat
    10561 ccgctggaag ttgagtacgt gcgttcggaa atgcaggtga aatttaccaa tatcaccacc
    10621 tcgccgaacg acattgtggt taacacgccg ttccatgtgg agattggcaa cctgaccggc
    10681 gaatttaata tctgtctgcc attcagcatg attgagccgc tacgggaatt gttggttaac
    10741 ccgccgctgg aaaactcgcg taatgaagat cagaactggc gcgataacct ggtgcgccag
    10801 gtgcagcatt cacagctgga gttggtcgcc aactttgccg atatctcgct acgcctgtcg
    10861 cagattttaa aactgaaacc cggcgacgtc ctgccgatag aaaaacccga tcgcatcatc
    10921 gctcatgttg acggcgttcc ggtactgacc agtcagtacg gcaccctcaa cggtcagtat
    10981 gcgttacgga tagaacattt gattaacccg attttaaatt ctctgaacga ggaacagccc
    11041 aaatgagtga catgaataat ccggccgatg acaacaacgg cgcaatggac gatctgtggg
    11101 ctgaagcgtt gagcgaacaa aaatcaacca gcagcaaaag cgctgccgac gcggtgttcc
    11161 agcaatttgg cggtggtgat gtcagcggaa cgttgcagga tatcgacctg ataatggata
    11221 ttccggtcaa gctgaccgtc gagctgggcc gtacgcggat gaccatcaaa gagctgttgc
    11281 gtctgacgca agggtccgtc gtggcgctgg acggtctggc gggcgaacca ctggatattc
    11341 tgatcaacgg ttatttaatc gcccagggcg aagtggtggt cgttgccgat aaatatggcg
    11401 tgcggatcac cgatatcatt actccgtctg agcgaatgcg tcgcctgagc cgttagtgat
    11461 gaataaccac gctactgtgc aatcttccgc gccggtttct gctgcgccac tgctgcaggt
    11521 cagcggcgca ctcatcgcca ttattgccct gatcctcgct gctgcctggc tggtaaaacg
    11581 gttgggattt gccccgaaac gcactggcgt taacggtctg aaaattagcg ccagtgcttc
    11641 gctgggcgca cgtgaacggg ttgtggtggt cgatgtggaa gatgcacggc tggtgctcgg
    11701 cgttaccgca gggcaaatca atctgctgca taaacttccc ccttcagcac caacagaaga
    11761 gataccgcag accgattttc agtcggtcat gaaaaatttg cttaagcgta gcgggagatc
    11821 ctgatgcgtc gtttgttgtc tgtcgcacct gtccttctct ggctggttac gcccctcgcc
    11881 ttcgcgcaac tgccgggtat caccagccag ccgctgcctg gcggtggaca aagctggtcg
    11941 ctcccggtgc agacgctggt gttcatcacc tcgttgacgt ttattccggc aattttactg
    12001 atgatgacca gtttcacccg catcatcatt gtttttggtt tattgcgtaa cgcgctggga
    12061 acgccctccg cgccacctaa ccaggtattg ctggggctgg cactattttt gacctttttt
    12121 attatgtcac cggtgatcga caaaatttat gtcgatgcgt accagccatt tagcgaagag
    12181 aaaatatcaa tgcaggaggc gctggaaaaa ggggcgcagc cgctgcgtga gtttatgctg
    12241 cgtcagaccc gtgaggcaga tttagggttg tttgccagac tggcgaatac cggcccgttg
    12301 cagggacctg aagccgtgcc gatgcgcatt ttgctcccgg cctacgtgac cagcgagttg
    12361 aaaaccgcat ttcagatagg cttcacgatt ttcattcctt ttttgattat cgacctggtg
    12421 attgccagcg tgttgatggc attggggatg atgatggttc ccccagccac cattgctctg
    12481 ccctttaaac tgatgctgtt tgtactggtg gatggttggc aattgctggt cggttcgctg
    12541 gcgcagagct tttacagcta gagaggcaaa atgacacctg aatcggtcat gatgatgggg
    12601 actgaagcga tgaaagtcgc gctggcactg gctgccccgc tgttgctggt ggcgctggtc
    12661 acgggtctta tcatcagtat tttgcaggcc gccacccaga ttaacgaaat gacgctgtcg
    12721 tttattccga aaatcatcgc cgtatttatc gccattatta ttgccggacc gtggatgctc
    12781 aatctgttgc tggattacgt ccgcaccttg ttcactaacc tgccgtatat catcgggtag
    12841 ccgtactatg ttgcaggtga caagcgaaca atggctatcc tggttaagcc tgtacttctg
    12901 gccgttactg cgcgtgctgg cgctgatctc caccgcgccg attctgagcg aacgcagcgt
    12961 accgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg ccccatcatt
    13021 acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg tgcagcagat
    13081 cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg tacgaaccgc
    13141 tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acgtttgtcg atccggccag
    13201 ccatctcaat atgcccgttt tagcgcgtat catggatatg ctggcgttac tgctgttcct
    13261 gacatttaac ggtcatttat ggttgatttc actgctggtc gatacctttc acaccctgcc
    13321 gattggtggc gaaccgttga acagcaatgc gtttctggca ctcaccaaag cagggagttt
    13381 gattttcctt aacgggctga tgctggcgtt accgctcatt actctgctgc tgacactgaa
    13441 tctggcatta ggtttactta atcgtatggc cccgcaatta tccatttttg ttattggatt
    13501 tccattaact ctgactgtcg gcatctcttt aatggcggca ttaatgccgt taattgcgcc
    13561 tttttgcgaa catttattca gtgaaatttt taatttgctg gctgatatta ttagtgaatt
    13621 gccattaata taattccgta acgtttatca tgttatccta aggattatcc gaaaaataat
    13681 acctacgaac attttccagg atactcctgc agcgaaatat ttgttttaag ctcactcaca
    13741 tatcgcaaca tttactttac tttaagacaa ttccaggcaa attatacaaa actttacggg
    13801 atagtaagtc cgcctgaaaa atcgcgagag tggcgcatta ggtgacccat gttgttccgt
    13861 ttagtcatga tgaaatattc aggtaagggg aattatcgtt acgcattgag tgagggtatg
    13921 ccatgtcaac gattattatg gatttatgta gttacacccg actaggttta accgggtatc
    13981 tgttgagtag aggggttaaa aaaagagaaa tcaacgacat tgaaaccgtt gatgaccttg
    14041 ccatagcttg tgattcacag cgcccttcag tggtgtttat taatgaggac tgtttcatcc
    14101 atgatgcttc taacagtcag cgcatcaagc acatcattaa tcaacatccc aatacgttat
    14161 ttatcgtttt tatggcaatt gccaatgttc attttgatga atatctattg gtcagaaaaa
    14221 atttattgat cagttctaaa tcgattaaac cggaatctct cgacgatatc cttggcgata
    14281 ttctgaaaaa agagacaacg ataacctctt ttttaaatat gccgacgtta tcattgagcc
    14341 gaaccgaatc gagtatgttg cgaatgtgga tggcaggtca gggaaccatt caaatctctg
    14401 accaaatgaa tatcaaagcc aagaccgttt catcgcataa aggtaatatt aaacgtaaga
    14461 tcaaaacgca taataaacag gttatctacc atgtcgtccg actgacggat aatgtgacta
    14521 atggtatttt tgtcaacatg cgctaacaca ttctgactgg tggtttccca ccagtcaggc
    14581 tgaattagat tactctgctt tctccacaaa gataccgtcc tgatgccctg cttcattaaa
    14641 gaaccagatg ccgagcgggt agtcttccag cgaaaccagg tacattgtgc cttcactaaa
    14701 ctcctcaact gccagtacca cgccaggacg acgcggaccg ccatccgttt tgactgttac
    14761 ccgatcattc accttcatcg ttttcctcct gtggctttgt gccagtgtag aacaatttcg
    14821 tttttctggc agcgccaggc gcgcgtgagt gctgattttc tcgacggtct atacttaaga
    14881 gatgccaggc ggacttaacg actggcggca acaacagagt aacggttgcg aggaaagatg
    14941 atgaaaaccg caaaagagta cagcgatacc gcaaaacgtg aggtcagcgt cgatgtcgat
    15001 gccctgctgg cggcgatcaa tgaaattagc gaaagcgaag ttcatcgcag ccagaacgat
    15061 tctgaacacg ttagcgtcga tggacgtgaa tatcatacat ggcgtgaatt ggcggatgcc
    15121 ttcgaactgg atattcatga cttcagcgtc tctgaagtga atcgttgaat gcacaataaa
    15181 aaaatcccga ccctgagggg gtcgggatga aacttgctta agcaagaagc acttaaaaaa
    15241 ttcgttacac caggaaatct gatgtgttca tcaccttatc cgcaattttt ttcgctgaca
    15301 agaaaatatt cgtgatatga atgattaacg ctacttatga atagaaatgt gacgcgcgtc
    15361 accccttggt ggctctggcg tcaacgggat cgcttccgaa aaaatcatct ttgataattt
    15421 gtgctgtttt gagaatcatc ctggtaaggg gagtactgcg caaccatgct ttcaattcaa
    15481 caaccactac tggtttttag cgatcttgat ggcaccctgc tggacagtca tagttatgac
    15541 tggcaaccag ctgccccctg gctcagccgt ttacgcgaag cgaatgttcc cgtcattctc
    15601 tgtagcagta agacatcagc ggaaatgttg tacttgcaaa aaacgttggg gctacaaggt
    15661 ttaccgctga ttgccgaaaa tggcgcagtg atccagcttg ctgagcaatg gcaggatata
    15721 gacggttttc cacgcatcat ctcaggtatt agccatggcg aaatcagcca ggtattaaat
    15781 acgctacgcg agaaggaaca ttttaaattc acgacttttg atgatgtcga cgatgcaacc
    15841 atcgccgaat ggacgggatt aagccgtagc caggcggcgc tgacgcagct gcatgaggcg
    15901 tcggtaacgc taatctggcg cgacagtgac gagcgtatgg cacaatttac cgctcgtctg
    15961 aacgaactgg gcttacagtt tatgcagggt gcgcgcttct ggcacgtcct ggatgcctct
    16021 gccggaaaag atcaggccgc caactggatt atcgcgacct atcaacaatt atcaggcaaa
    16081 cgcccaacca cacttggcct gggcgatggg ccaaacgatg cgcccttact ggaggtaatg
    16141 gattacgcgg tgattgtgaa agggctaaac cgtgaagggg tgcatctgca tgatgaggat
    16201 ccgacccgcg tctggcgaac gcagcgtgaa ggaccggaag gctggcgtga agggctggac
    16261 cattttttct ccgctcgtta agcgttatcg ctcgcgcata cccgattacg cccggcctgt
    16321 ttagcgagat aaagccgacg gtcagccagt gactggagtt gttcaaaatc ataatcaccg
    16381 gtttcctcgc tgctacttac ccccaacgag gcactgatgc gtatcgtcgt actcttagca
    16441 atcaacatct ctttttcatt taacttcagg cgaatacgtt ctgcgacttc cgcagcctcc
    16501 gtcagactcg cgcctggcag aatcacacaa aactcctcac caccgacccg cccggcaacg
    16561 tcctgcgcac gcaaggaact gctaattaat ccagcagcat gagaaagaac acggtcgccc
    16621 gcctgatgac caaagcggtc attaatcgct ttaaaatggt caaggtcgac ctgaatgaca
    16681 gaaaaaggat gttggtgcgt ctgacacaat ttcgcgagcg gacgggcttt ttcaaacagt
    16741 gcgccacgat tatataaacg ggttaaggtg tcgtgccacg cctgccactg caacgaactt
    16801 tgcagaacat acatgttgct gaccatccgg cgaatcacat accaggagat gagtaacatg
    16861 gtggtaaaga gcgcccacag cagggttaat gcaatgctga tactgccgaa atcgccgcgc
    16921 acgccttcgc ttagcgtatg gacgcgcacc agcacaccgt cgaaatggtc cagacgttcc
    16981 cagctaacat agcgactgtc catacgaatg ccgccccgcg tgtcatgttc cattgcctgc
    17041 gccagcaagg ccagttcgcg aggatcaaaa atattccctg ttggatggtc gggattggaa
    17101 gaggtcaata atctcagctt actgtcatag agctgatact caccatcgag gtttttatcg
    17161 atggcgtttc ttaaaaattg ctgcatagta cgcacaggaa tactcatccc cagcacgcca
    17221 taccagtagt tattactgtc taccggaaca ctgacggtaa cctgcggttc agtattgctg
    17281 gcatgttccg gttgcgaagt gaaccagcgt accgcgcggt gacgattttc tcgttgtgaa
    17341 tggccgataa accagggttg ggtgacatag ccgtaataac gcgtcgggac attgcgcgta
    17401 aacaaggtcg gctgcgtcga aacgtaaaat ccggcacgcg agacatacat cgcctgttca
    17461 accatcgacg aggagttgtg tgccagtcgc agcaagtaac caacttccag tgcagcggta
    17521 atttcattgt cgaggctttc attttcgcga gacaggagat ttccctcgct gactaatgca
    17581 tccgatacgc cattgactgg cagggtgcgt cgtcggttga gttcgatttt ccaggcgtgc
    17641 tcgtcgcgat gctgttcgaa ctcggttaca gcatcacgca gagaggtgaa atccagtggc
    17701 gctacgagag cttcgcgcat gccattacgc aaaaagatca gtttgtcgac gttatactgc
    17761 aaatgcttat cgagcgcgtt ggctacgttt tccagatgat tacgctggct ggagatatag
    17821 gcatcttcca gcaccactac ttcacgccag gtgagcaagg tggaaaaaag caataccacg
    17881 ataaagcaga gattaacgac atgaccaggc cccaggcggc gtgcgagttt tttcaaccag
    17941 ctctggtttt ccatttttgt ctcgtgctgc accctgacga ctcccaacct gcttttttat
    18001 gattctggca taacctggcg atagcgtacc gcaaaacagc gataatttta taatggtggc
    18061 atgataagac gcgactatca ttcattgcag gcattggcac accaggccgg atgtgatccg
    18121 gcctggtgtt aatgataccc tttcaaactt cttccgcaga acgactcccc tcccccggaa
    18181 caagctcggt ttcatgaaaa gcttcccgct tgaccccgta accgtcatac caacgactct
    18241 caaccatacc gctggagtat ccagtgacaa tcatccgcgg gccgccctct ttaaccgtaa
    18301 cttcctcact aaccataaag ctcatactcg cctccttttt tcgagtgaaa cttgttcacc
    18361 ttagttgaag atggcgaatt ttgcataata gcctgtgcga gtattaatgt gcctgaccgc
    18421 gcattttcgc tacggctttc actcccagca ccacgatgcc gccgatgata aatccaagaa
    18481 tcagatttaa aacagtcggt aatatcatcg ccaccactgc actttgctgc ccggcgaaat
    18541 gttcaatggc gtgatgcagc ggcgcaatac catgtaccac aatcccgccg ccgacgagga
    18601 acatcgccag cgtgccgaca atcgataacg ccttcatcaa ccagggcgca atgatcaata
    18661 accctttacc taatgcctgc atcagcgcgc tggatttttc cgccagccaa taccccaggt
    18721 catcaatctt aacgataacc cctaccagac cgtagacgcc cacggtcact accagcgcga
    18781 tgcctgaaag caccagcacc tgattaagca acggcgcttc ggccacaatc cccagcgtga
    18841 tggcgacgat ttcggcagac aatataaaat cggtacgaat cgcccctttt attttgtcct
    18901 tttcaaactt cagcggatcc tgcgccgcca gcttctccag acgctgctgg ctctgcgccg
    18961 gatcttcttt atgtttacgc gcctccagca tatgcagcac tttctctact ccttcaaagc
    19021 agagaaacgc gccaccaatc atcaacagag gcgtaatcgc ccacgggata aacgcactga
    19081 tgatcagcgc cagcggcacc agaatcactt tattaatcag cgatcctttc gccacgcccc
    19141 agaccacggg aagttcccgg ttggcccgca cgcctgaaac ttgttgcgca ttgagcgata
    19201 agtcatcccc taatacaccg gcggttttct tcgccgccag tttgcccatc acggagatat
    19261 cgtccagtag tgtggcgata tcatcgagca acgtcagtaa actactcccg gccaaaagca
    19321 ttccctctca tttttattgt tgaagggtgt aagtatgaag caaaaacggc agctctgata
    19381 cttgcttcac aggtcaacat ttttttaaca atcaagtgca attatatctc tcgccagcag
    19441 aatgattttc acgtttacta tgaaccacct ttttatttcc accgtgaggg attatgcgtt
    19501 tcggccagtt gttaccgctt tttggcgcgc tgtttgcgtt gtatatcatt tggggctcaa
    19561 cctattttgt cattcggatt ggcgtggaaa gctggcctcc gttaatgatg gcaggcgttc
    19621 gattcctggc agccggtatt ttattgctgg catttttgct actgcgcgga cacaagctcc
    19681 ccccgctacg tccgctgctc aatgccgcgc tgattggcct gttattgctg gctgtcggta
    19741 atggcatggt gacggttgcc gagcatcaaa atgttccttc cggcatcgcc gccgttgtgg
    19801 ttgcaaccgt gcccctcttt accctgtgct tcagccgcct gtttggcatt aaaacgcgca
    19861 aactggaatg ggtgggtatc gccattgggc ttgccggaat catcatgctc aatagcggtg
    19921 gaaatttaag cggcaatccg tggggcgcga ttctgatttt aatcggctcg attagctggg
    19981 cgtttggctc ggtttatggc tcgcgcatta ccttacctgt agggatgatg gcgggtgcga
    20041 ttgagatgct ggcggcaggc gtggtgttaa tgatcgcgtc gatgattgca ggtgaaaaac
    20101 tgacggcgct cccttccctt tcaggcttcc ttgcggtcgg ctatctggcg ctgtttggtt
    20161 cgattatcgc cattaacgct tatatgtatt taatccgtaa tgtcagtccg gctctcgcca
    20221 ccagctacgc ctacgttaac ccggtggtcg cggtcttgct gggtacggga ctgggcggag
    20281 aaacgctgtc gaagattgaa tggctggcgc tcggcgtaat tgtcttcgcg gtggtgctgg
    20341 tcacgttggg aaaatatctc tttccggcaa aacccgtagt tgcgccagtg attcaggacg
    20401 catcaagcga gtaaatgaat cccctgcgtg tcgatctgcg cgctggcacc ttcgccgcag
    20461 atccactctt ccagacgctc gctaagcgct tcatccgtca gcttctcgcg cccacgtaac
    20521 gcacactccc agacaatcag tacgcgccag ccaagctcct gcaagcgact gatatcacgg
    20581 cgatcgcgct caacattttt acctatcttc tccagccaga actcggttcg agtcgcaggc
    20641 actttaaaca gatagcagtg atgatgatgc cagaagcagc cgtgggtaaa tatcacgcaa
    20701 cgatattcat caacgacaaa atccgggcgt ccgggcagac tggcgtcctg aacgcgaaac
    20761 gccaggccct gcccggttaa cagactggcg aggcgcttct caatcgccgt atcacgcgtg
    20821 gcaatcgcgc gcatattttt gctgcgagtg gccttatcgt gaacgtcggc catgttgtgc
    20881 ctcttgctga cgcaacgcca ccgcctgttt gatttttggc tcaagcagtt ttgccaccgc
    20941 ggcaaagacc ggcacgacca ccgagttacc gaactggcga taggcctgag tgtccgaaac
    21001 cggaatgcgg aatttcgctt ctcccggcgc ttcaaaaccc attaagcgcg cgcattcacg
    21061 aggcgttaac cgacgtggac gatgttgctg attctgcgga tcgtcaaagt ctttctcacc
    21121 tttggccata tcccagccgc gatcgattaa aatttccgcg ccatctttgt aataacgcgc
    21181 agacagcgta cgcgtgacgc tttgcggatt gttcggataa accattccat aaccgaagcc
    21241 gttaccgcgc gcctgatgtt ttttcgcata tcgatagagg tacttccaca gcaccggcgt
    21301 caggatatat ttcgcctcga ccatcgggtc cagcagctgc gccagcgtca ctcgctgcgc
    21361 agggaaacat tcgctgatat cacgcagggt aaaatcggct ttcagattca gatcgcgacg
    21421 aaaacccacc agcacgatgc gttcacggtg ctgcggcaga aaatgtttgc cgtcgatgat
    21481 tttcggatcg tccggcccgt tatcttctgc atcagccacg tcatagccca gttcgtccag
    21541 cgtctgcatg atgatgcgga acgttttacc ctggtcgtga cttttcaggt ttttgacgtt
    21601 ttcgagcaca aacatcgccg gacgacgcgc gtcgataata cgcaccacat caaaaaacag
    21661 cgtgccctgg gtatcgcagg caaaaccgtg cgcccgcccg agcgagttct ttttcgatac
    21721 gccagccagc gaaaatggct gacaagggaa accggccagt aaaacatcgt gttcaggaat
    21781 gtgttgacga atatgttccg ccgccgcctc atcactcacg ccttctttat ggctgagggt
    21841 gatgtcgcgg atatcttcat taaaatgatg cgtcgccggg tcgcaataat gattggcttt
    21901 ataagtgcgt accgcatgtt tgttccattc gctggtaaac acgcactgtc cgccaatcga
    21961 ttcaaaaccg cgacggatgc cgccaattcc ggcaaataga tcgataaagc gaaacgcata
    22021 atgcggatga tgtgccggtg gtttgggtaa caacgtttgc agatgggcga actctttctc
    22081 acttaaacga tgccatgccg agtcattcgc cagcgcacgt tttaaaattg ccgcgctcca
    22141 gtgattctca cccacaccat taagctgcgc caccagcgtt ttaacatcat aaatttgcag
    22201 cagtttctcc agcattgcct gtgcggcatt cccggtgctg tatgaatcgg ttactgatat
    22261 attttcctgc atagatttaa ccggccatct gagatggccg acaggttaac ataattacag
    22321 ccatttaggg ccacgcgctt atcccgcggg tgaaaacgca tctatcacct tgtgatcgac
    22381 accttcattc tcccccgcca gttcggcact gagcttcgcc ataaactcga ctaaaaaatg
    22441 cgcgttgtgc tgcgccagct gcttgcctct tacggtttgc atggtttgcg gtagcttcag
    22501 caacttagtc tggaaatgat ccagcgcgta gcgtttatca tcaagcgggc gatgctgtgc
    22561 gagcggatct tcgccatcaa acagcgctac gcccaatgcc cctgaaacgg caaacacgcg
    22621 cgccaggcca atcgccccca gggcttccag ccgatccgca tcctggacga ttttagcttc
    22681 cgttgttaag ggggcgattt gcgcgctgaa actgtgagcg gcaatggcat gacaaacggc
    22741 ctcgattttc tccgccggaa attgctcgaa ctcttcacgg agcaggcgac gcgtctcttc
    22801 tgccgccagg attgaagaac gctgcctttg cggatgattt ttcgccaggc tcacaatatc
    22861 gtgaaaataa caggcggtta aaatcaccag catatcgaca tcgtcatctg ccgccagctt
    22921 ctgtgccgta gcccagacgc ggcgaaaatg acacacgtca tgggccgcgt cctggtgctg
    22981 atgatggttt ttcaaccagt tttcgaactg tgcctgccag tgttgtaagt ccatacgcgc
    23041 tccgttgcca aagagtcgcc accttagcag tttttattca tagcgtctgc tacgtgcggg
    23101 acgaggtcgg gtgtaccagg caatgctgcc taacaatgcc ccgaccagag ccataaataa
    23161 aaatccgacc agtgccccta accactttcc cgcggtagag cctaaatcgc tttcaaatcc
    23221 cgacacgggg gcatcgggga tccggctcat cacccagatg taacgcgcca ttgcccagac
    23281 gaaatagcca caccatgtat agaaagccca caaagccagt ttgccgccag ggctgcgaga
    23341 gagtctctct tccatctttg tcgaaccgta ttccattttg agataacccg gaaaattcag
    23401 acttagtact attatgtgat aaaagtcaca tttttccaca ttggatgagc cagtcaagac
    23461 tcaatcattt tttgaacaaa tagataaatt ttgcgcaacg gcagtgaata caggaaaaat
    23521 cgttttgagc gattttattt atctttgata tcggctttat taattcattt aatcaatata
    23581 ttagcactga ttacaatta
//
LOCUS       DAZGTR010000069        23260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGTR010000069 DAZGTR010000000
VERSION     DAZGTR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.069"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            73..579
                     /locus_tag="ACHLCY_002966"
     CDS             73..579
                     /locus_tag="ACHLCY_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370182.1"
                     /translation="MKLYKSLYSFLLMFSFLPLSAMAGSTVWTVGGEQGWREISATND
                     DGYTINFSCDAGAREGSEDHIAGRNLYVSGGKENADFSTRDTISRKADVITLIVGPDS
                     FNIGTQNTAPNRREWYSFWKAVSSTNEKKIDVYIGSSRITSFTLDGISSIYTEAKNDG
                     CLKQDDGE"
     gene            581..1750
                     /locus_tag="ACHLCY_002967"
     CDS             581..1750
                     /locus_tag="ACHLCY_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000217774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HFP0370183.1"
                     /translation="MKDIKKLALGSVSDLYNRDSNITWRDNLSKIKMPGYTSASDLLK
                     SEYASNNLVRQLAELQKNAIIPSYREHMANLQEQLRIGRLAQEQIARLNLQSLLSDSV
                     KKQLANMTSLRRINSKHREFLEILQKQAKVYPPHDANKYIQMLRKQAEIAMPLRNQFE
                     MLNKQAGLNNMQAILNELRQNVSHKIDLNNEIKESLKQYTLAQKASLHQIMEQSLSSI
                     AQAYVEGAIETSHNESDVQDKGLNKSSPTFIDSFKSLPHPLQFIIMWLLTEVVLGAIA
                     DYAKEQILSQIHKTESYSVSLYEDAPISKQKLIKENTEIKWEDLNGFRFITGDNVRLH
                     VSPSLNSEVIECIGKNTIVAILDKKDRQWLYVQVKSGDEFITGWITRTYTKPLKA"
     gene            1905..2471
                     /locus_tag="ACHLCY_002968"
     CDS             1905..2471
                     /locus_tag="ACHLCY_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370184.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRFTDQTCKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            2482..3093
                     /locus_tag="ACHLCY_002969"
     CDS             2482..3093
                     /locus_tag="ACHLCY_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370185.1"
                     /translation="MGLNVASVKSYISSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TGDGLVLDWFKTAKSRVINPDGTVNIPSKYLLNVRIYRLLSSGLTKLENEMTVFPVTT
                     GDVTYARDQVTEFKSFPMTFALHSTFNQSSSSLASLLGFSFSL"
     gene            3108..3989
                     /locus_tag="ACHLCY_002970"
     CDS             3108..3989
                     /locus_tag="ACHLCY_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HFP0370186.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKYDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQDTKESTEGAYTRLWVPFRATDFIPQVGIEKLS
                     DLSVQPGFVWGYTDSGR"
     gene            4071..7445
                     /locus_tag="ACHLCY_002971"
     CDS             4071..7445
                     /locus_tag="ACHLCY_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0370187.1"
                     /translation="MERKNANIDDVIRTVETASAKELEELAGIREAVEDLKGERVATV
                     DPVSRSVSALNRTIENSRPDFVANAPSVDPIVDAMKRLNLGDVSRVVQEDVDLQEPQA
                     KSTTRKGKKRRKKAITEDVKAQRTEAAEHAREMFGQKGGAQKSQNQRDARGRFIGKSG
                     SKAAAEDARAERAEKAWRKEDDERLNAESGLLKKLSKVAEGIGNPSETRAVDALGYAV
                     AGPLWAAGKELGGISKEVGGSLNGARKSIADVIRGNDDNSRRKGFFRRKSQNSADVVQ
                     VNTQKRTVQELQEQTSEIKEGNDKILRALDQIAKNTGKKKGGLLSKLFSLLGKGAGGV
                     ASLLMGRGMLKKAGALAFGALGAKKLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAV
                     GKGALRAIPLVGTVAGGIYGAVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLD
                     MGGLVSGISSAIGEVLKSLGFEDIGNMLQSFSTESIALAIDSGITNLETYISNLGDTI
                     STKFDDYTAKIGDAVSAWFSDTSNKLLEKLDAIKDFFTVDNLKQVFSDAIDSAIDFIK
                     NPGKHIKEAAGNIWDGVKNLPGKALDAAVDAVKNTPAAMIVSKIPNPIGEANAKEITP
                     ESKVPVNSHQGTSDSKTESDAKQTNIAARVIYAALDMAKDSNKTVKETANQIINANAV
                     ETGNKAAQTIDAALGQSATGKEEALSAYEIDKRRFNNGKDVSLPKLNAAGYQWISDNA
                     DYFDELERKYGLEKGILSAVASAESSAGQRTGNPVDKNGNKLSSALGAFQITKGTRED
                     LGLSDADAMDTRKAADGAARYLSMLMNRYNGDQGRAIAAYHAGMGHVDKGRVVAGTGE
                     YVTRVRGYQQMLNNGAVYGSKVDHSAPAIYEKIPDNAVIDQSTGLAFTPGDSPFEKGG
                     LVDKIGNAVGVNDLVNKFMNGRGMRREVVQGTLEERARGKGTATAAGNVYVDTPMPVE
                     EARPVANNSSYFDHLGAQMGIDGLFDKLRNSPGMRKNNAPEPASTSQVTTAANDLQQP
                     TGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETKRLFAQMTSLLARIEEHTKDSA
                     KGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
     gene            7445..7801
                     /locus_tag="ACHLCY_002972"
     CDS             7445..7801
                     /locus_tag="ACHLCY_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370188.1"
                     /translation="MANNNEIDPLLTLELSGVKTYESQEEAWGARLYEWLNTYQGEVY
                     GDPSWGNVLPQFKHEPTNLSHVQIAVEAMLLQKLTVDLPDIPISGLSVAEGDAFDKLK
                     ISIRIRDITITQDVVL"
     gene            7798..9231
                     /locus_tag="ACHLCY_002973"
     CDS             7798..9231
                     /locus_tag="ACHLCY_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HFP0370189.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAANLANLISVKTTTPITAGTDAETT
                     EITRNRAQYYLAYDDQVVWGGDYTYFLVRNIPGLSWVKAWGEGQQEKLDGAYNVRNIN
                     KIFISGWHPNKSQSELEEMILAAFKKVPNELNKKFSYKEVRKLPFKITITGRISASLT
                     IENVTDELKSALETKFGRDSTFFDPNRVGKYILIKKKDVWAFIETLGYFRDFYLEFVE
                     WNESNGFYDFVYLDTENSTFNISYEEE"
     gene            9231..10067
                     /locus_tag="ACHLCY_002974"
     CDS             9231..10067
                     /locus_tag="ACHLCY_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370190.1"
                     /translation="MQRSWFNNRLTSAKQKSLLYKSLADLVQSMMDTFVDPWLERITN
                     RKSIFSMSKEDLETRTNELGQFFTIRTSDSSSVPMLLQQRLDEIHFKGTERPINQTIY
                     REFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFLTSRGMISIPIND
                     LARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLSVVVNEQADMITL
                     NEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTFDRTRADDLLLDS
                     DA"
     gene            10146..10580
                     /locus_tag="ACHLCY_002975"
     CDS             10146..10580
                     /locus_tag="ACHLCY_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370191.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            10592..13441
                     /locus_tag="ACHLCY_002976"
     CDS             10592..13441
                     /locus_tag="ACHLCY_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370192.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQRTDIPLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVTRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSERINRTIGIDISDSVTSSRSDVAASSLAVKKAYDLANSKYTAQDASTTQ
                     KGLVQLSSETNSDSETMAATPKAVKSVKDLADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAGLATGANKLPYFTGTDTISQTDLTSVGRDILAKTSVLAV
                     IQYLDLRELGTSGEKIPLLSTANKWSARQTFNGGITGALTGNADTATKLKTAININGV
                     RFDGSADININTLVSRGRVTALEANAQGTSGIQLYEAYNNGYPSPYGNVLHLKGATAA
                     GEGELFIGWSGTSGDHAPVHIRSRRDTDSANWSEWAQVYTSKDSIPGVNAKGDQDTSG
                     NAATATKLQTACTINGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPWNAESGA
                     YNVIRSADSYILVNFYTGVGSCPTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSK
                     NLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIK
                     GKPGSGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKTTSSFDYGTKTTNS
                     AGNHSHNIPVGHTGAGNGVSAGFNAALGTGTTSSAGGHAHNVYIGAHNHTIGIGAHAH
                     SVIIGPHGHTITVNATGNEENTVKNIAFNYIVRLA"
     gene            13441..14019
                     /locus_tag="ACHLCY_002977"
     CDS             13441..14019
                     /locus_tag="ACHLCY_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0370193.1"
                     /translation="MTFRMSEQSRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWNLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIRKAEETKNSLMQMASEHIAPLQDAVDLDIATEEE
                     ASLLTAWKTYRVLLNRVDTTVASDVEWPVAPQ"
     gene            complement(14063..14635)
                     /locus_tag="ACHLCY_002978"
     CDS             complement(14063..14635)
                     /locus_tag="ACHLCY_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFP0370194.1"
                     /translation="MLSYLMAIHFVLFGDSTNLKNFWKYEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDMPGSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
     gene            complement(14792..15280)
                     /locus_tag="ACHLCY_002979"
     CDS             complement(14792..15280)
                     /locus_tag="ACHLCY_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370195.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTGTNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSATSSVKG
                     LL"
     gene            complement(15293..18100)
                     /locus_tag="ACHLCY_002980"
     CDS             complement(15293..18100)
                     /locus_tag="ACHLCY_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0370196.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVAVSDA
                     NGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLGSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SRVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLNPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            complement(18087..18242)
                     /locus_tag="ACHLCY_002981"
     CDS             complement(18087..18242)
                     /locus_tag="ACHLCY_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0370197.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            complement(18251..18625)
                     /locus_tag="ACHLCY_002982"
     CDS             complement(18251..18625)
                     /locus_tag="ACHLCY_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370198.1"
                     /translation="MKDIDTETRNNTVAGDVTAGEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(18681..19193)
                     /locus_tag="ACHLCY_002983"
     CDS             complement(18681..19193)
                     /locus_tag="ACHLCY_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0370199.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFVGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGKDIVP
                     DRIRSALGLG"
     gene            complement(19193..20377)
                     /locus_tag="ACHLCY_002984"
     CDS             complement(19193..20377)
                     /locus_tag="ACHLCY_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0370200.1"
                     /translation="MSETRFHGARVTESTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCYGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            20535..21644
                     /locus_tag="ACHLCY_002985"
     CDS             20535..21644
                     /locus_tag="ACHLCY_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0370201.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKRGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            21870..>23260
                     /locus_tag="ACHLCY_002986"
     CDS             21870..>23260
                     /locus_tag="ACHLCY_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFP0370202.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAIKYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNGAIEVRVDAN
                     NLTNPVIKTFKLK"
BASE COUNT         6389 a         5493 c         5642 g         5736 t
ORIGIN      
        1 aaattctctg ggcatagtgg atctaacaat atggactatg cctataatat ctaaaaacaa
       61 aaggataaaa atatgaaatt gtataagtca ttgtatagct ttttattaat gttctctttt
      121 ctgccattat cagcaatggc aggttctacc gtttggacgg taggaggtga gcaagggtgg
      181 agagaaatct ctgcaaccaa tgacgatggt tacacaatta acttttcctg tgatgctgga
      241 gcaagggaag gttcagaaga tcatatcgct ggaagaaatt tgtatgtaag cggaggaaaa
      301 gagaatgccg atttttctac acgcgacaca atatcacgta aagcagatgt aattacttta
      361 attgttggcc cggatagttt taatattggc acgcaaaaca ctgctcctaa tcgtagggaa
      421 tggtattcat tttggaaggc agtgtcatct acaaatgaaa aaaaaataga tgtatatatc
      481 ggttcgagtc gaatcacatc attcactctg gatggtattt ccagtattta tacagaagct
      541 aaaaatgacg ggtgtttaaa acaagatgac ggtgaataaa gtgaaggata ttaaaaaact
      601 tgcacttggc tcagtaagcg atctctataa tagagattca aatattacct ggcgtgataa
      661 tttaagtaaa atcaaaatgc caggatatac atcggcaagt gatttattaa aatcagaata
      721 tgcttctaat aatttggtta gacagttggc agaactgcaa aaaaacgcaa tcattccatc
      781 gtatagagaa catatggcaa acttgcaaga acagttaaga atagggcggc tagctcaaga
      841 acaaatagca agattgaatt tgcaatcgtt acttagtgat tctgttaaaa aacagcttgc
      901 gaacatgacg agcttgagaa gaataaatag caagcatcgt gaatttcttg aaattctaca
      961 gaaacaggcc aaggtttatc ccccgcatga tgcaaataaa tacattcaaa tgctaaggaa
     1021 gcaggcagaa attgctatgc cgttacgaaa ccagttcgag atgcttaata agcaagctgg
     1081 tttaaataac atgcaagcaa tacttaatga attgcgacaa aatgtaagtc ataagataga
     1141 tttaaacaat gaaataaaag aatcattaaa acaatatacc ctagcgcaaa aggcttcatt
     1201 acatcaaata atggagcaaa gtctttcatc aattgcacag gcttatgtcg aaggtgctat
     1261 tgaaacatct cacaacgaaa gtgatgttca agacaaagga ttaaataaat ccagtccgac
     1321 gttcattgat tcatttaagt cgctccctca cccgcttcag tttattatta tgtggctttt
     1381 aacagaagtg gtgcttggcg ctattgctga ttatgcaaaa gaacaaatct tatcgcaaat
     1441 acacaagaca gaatcatatt ctgtatccct atatgaggat gcaccaatat caaaacaaaa
     1501 attaattaaa gaaaacacag aaattaagtg ggaagatctc aatggtttta ggtttataac
     1561 tggtgataac gtaagattac atgtcagtcc ttctttaaat agtgaagtga ttgaatgcat
     1621 tggcaaaaac acaattgttg ctattttaga taagaaagat cgtcaatggc tttacgtgca
     1681 ggttaaatca ggggatgagt ttattactgg ttggattaca cgaacataca caaagcctct
     1741 taaggcttga attttatctc cgcatcttcg gattgggtgt tgatgacgat gtgctacttg
     1801 aagcacttga gttgttttaa cgtagtatct gaaatgtgta gactgaccgg taacaaatga
     1861 caactcgtag aatcggttaa cacaccagat tctacgaggt ttcaatgaca ccacgacaat
     1921 tactcgaaga cgtcaaatcc cgcttcacac ctttgattgc ggatgaacct gctttactgg
     1981 aatccctgct aagaaaagca ttgggaacct accaggatag ggcggggcac atcaagcgga
     2041 tacgcttcac tgatcagacc tgtaaatcac ttgcttgccc agctgatttt cttgcgctcg
     2101 tatcggttac agatcacacc ggcgatcttg tctactccga tgtttacgat gggaatatcg
     2161 agcttgaaga tactcatcga gcggtatacc cgctgaatgt gtcatatctg gctaatttac
     2221 gtgatatgga tctggataat ggggaagtgc cacctgaaat cattgggtta ctttctgact
     2281 atctggaagt gttaatcgcg atacctaaca ctgatcgcct gcgaagaata tctatcgcgg
     2341 ggaaactcga tgccagcaat ttatccgacg agaacacgct gtatcagcga aagctggatc
     2401 tggaagagaa aatgagcgca acaagggcaa ttatcccggg aattgttctt ttctcatcca
     2461 tgttgaagtg aggtcgttga tatgggactt aatgttgcat cagtaaagtc ttatatatct
     2521 tcggcattaa cgacgacatt atttggctcc ggcgttggtg agcgggaagt tggtaagctg
     2581 acgtcaatca tcatgaacaa aatgctgttc gcgcaaggat ggcagttctc tgtcgaagtt
     2641 gatgggctgg agggggcaga cttctttgcc aaagacatta cctaccacga ttacagcatc
     2701 gaatatgaaa cgattaaaat cggcggaggg aatatccttc agccaacgga gcgttcgcct
     2761 gggcagataa caatgatggt cagggatacc ggtgatggcc tcgttttgga ctggtttaag
     2821 acggcaaaaa gtcgggtgat caatcctgac ggcaccgtga acataccgtc taaatatttg
     2881 ctcaatgtgc gtatttatcg gttgctgtct tccggtttaa ccaaactgga aaatgagatg
     2941 acggtattcc cggttactac cggcgatgtc acctatgcgc gggatcaggt tacggaattt
     3001 aagtcattcc caatgacctt cgcattgcac agcacgttta accaatcctc aagttctttg
     3061 gcttcccttc tgggctttag cttttctctt tgaattaagg agcaaggatg cttttacctc
     3121 ttttcccgct accatcgcgg ccaactgaat tgatccagtt ccgtcagcca aatattgctg
     3181 atgcgatgcg tttcaactcg ataacaccgg aggaacaaga acaacagaca acggcgtatt
     3241 taaaagcctt gctggctgaa cccgcgaaat atgatcccct gacatggacg gcgcaggacc
     3301 ggattaccgc gttatggtgg atatttactg gctcccgtga aacaccggtc gagacattca
     3361 cctacacctg taaacattgc ggtaaagagc attattacga ttgtgatatg aatgctctgg
     3421 ctgaagatat ccaggtcctg gaagtggaac ctttcattga cgatattgag gtgtctgtag
     3481 agggagtacc ttatcaatgg cgtatcgtgc cgcttgatgg ttgggcaatg gaaatgctgg
     3541 agatgcgccg tgcagcattg ccacctgaag acgacgcgga attcaaagaa gcgatcgttg
     3601 atttgcgttt ttgggaattc gcttatcagt gtgagcttta taacgatgtt agtggtactc
     3661 gtgaagatca ggctgagcgt cgttatgaaa cgatcaaacg gatggccatt gatactgaat
     3721 ttatgaagct ggcggcacac atccggctgg ctcatgaaaa gctcgaacat ggtttaccgt
     3781 gctacatcga taaaggcgaa atgcgtcttc gtctcccgcc gcacaaatgc ccaaaccagg
     3841 atacaaagga gtccacagag ggtgcgtaca cccgtctgtg ggtgcccttt cgggctaccg
     3901 acttcattcc acaggtgggg attgaaaagc tatcagacct tagtgtccaa cctggttttg
     3961 tatgggggta taccgattca ggacgctgaa aggcttactg aatcctatgc gtttttcctg
     4021 ttggagaaac tggaagaaaa acttaaaccg aaacggtagg cgataagatc atggaaagaa
     4081 aaaacgccaa tattgacgat gttataagga cagttgaaac cgccagcgcg aaagagctgg
     4141 aagagcttgc aggtattcgg gaagctgttg aagatttgaa aggggaacga gttgcaactg
     4201 ttgatcctgt ctctcgcagt gtgtcggcat taaatcgcac aatcgaaaat tcccggccag
     4261 actttgtggc caatgcgcca tcagtagacc ctattgttga cgcaatgaaa cggcttaatt
     4321 taggggacgt ttctcgtgta gttcaggagg atgttgatct acaggaaccg caggccaaat
     4381 caactacgcg aaagggtaaa aaacgacgca agaaggctat aacagaagat gtaaaggcgc
     4441 aacggaccga agcagccgaa cacgctcgcg aaatgttcgg tcaaaaaggc ggtgcgcaaa
     4501 aaagccaaaa ccaacgcgat gcgcgtggtc gttttattgg aaagtcaggg agtaaggccg
     4561 cagcggaaga tgcccgtgct gaacgtgctg aaaaggcctg gcgcaaagag gatgatgagc
     4621 gtctaaatgc tgaatcaggt ttattaaaaa aactgtcaaa agtagctgaa ggcataggta
     4681 acccttcaga gactcgtgcc gtcgatgcgt taggttatgc cgttgctggt ccattgtggg
     4741 cagcagggaa ggagcttggc gggatatcaa aagaagttgg tggatcgctt aatggtgcca
     4801 gaaagtctat tgccgatgtg attcgtggca atgacgataa cagccgtaga aaaggttttt
     4861 ttaggcgtaa atcgcaaaat agtgccgatg tcgttcaggt taacacccaa aaacggacgg
     4921 ttcaggaact tcaggagcag accagcgaaa ttaaagaggg caatgacaag attctccgtg
     4981 cccttgatca gatagccaaa aacaccggta aaaagaaggg cggcttgctg tccaaactat
     5041 ttagcctgtt agggaagggg gccggtggcg tcgcgtcgtt gttaatgggg cgtggcatgc
     5101 tgaaaaaagc tggagcactc gcttttggcg ctctgggggc aaagaaactt gtaggaatgc
     5161 tacgcggtgg tggcaagaag actctcgctc atgaaggcgg agatttggct gcccgggcag
     5221 caggtaaact tggattaaag gcagttggta aaggggcgtt acgcgcaatt cccctagtcg
     5281 gcacagtggc tggaggtatt tatggtgcgg taaccggttg gaatgataca gaagcgcaac
     5341 gtcgagcgtt tgggcttaaa tcaggacaag atccatcatt ccagcaaaaa gccgcttata
     5401 cgttagctaa tgttcttgat atggggggac tggtatctgg tattagcagc gccattggtg
     5461 aggttctcaa atcacttgga tttgaggata tcggcaatat gttgcaatca ttttcgacgg
     5521 aaagtattgc cctggccatt gatagtggga ttaccaactt agaaacatat atttctaacc
     5581 ttggcgacac catttctacc aagttcgatg attacacagc aaagattggt gatgctgttt
     5641 cagcatggtt tagcgataca tctaataagc tgcttgaaaa gctggatgcc atcaaagact
     5701 tctttactgt cgataacctg aaacaggttt tcagtgatgc aattgatagt gcaattgatt
     5761 tcattaagaa cccagggaaa cacattaaag aggcggctgg taatatttgg gatggggtta
     5821 aaaatttacc aggtaaagca ttagatgcag cggttgatgc cgttaaaaat acccctgcgg
     5881 caatgattgt atcaaaaata cccaatccga tcggcgaggc taatgcgaaa gaaatcactc
     5941 cagagtcaaa agttccggtt aatagccacc aggggacatc tgattctaaa actgaatccg
     6001 atgccaaaca gactaatatt gctgcccgcg tgatatatgc ggccctggat atggcgaaag
     6061 atagcaataa aacagttaaa gaaactgcta atcagattat caatgcaaat gccgtagaaa
     6121 caggaaataa agcagcacaa acaattgatg ctgccttggg ccagtctgct acaggtaagg
     6181 aggaagcatt aagtgcatat gagatagata aacgtcgatt taacaatggc aaggatgttt
     6241 ctttgccaaa attaaatgct gccggatacc aatggatttc tgacaatgcc gattattttg
     6301 atgagcttga acgtaagtat gggcttgaaa aagggattct gtcagcagtt gcatccgcag
     6361 agtctagtgc aggccagaga actggaaatc cagtagacaa aaacgggaac aaactttcat
     6421 ctgcccttgg ggcttttcag atcactaaag gtacaaggga ggatcttgga ctcagcgatg
     6481 ctgatgccat ggatacacga aaagcagctg atggtgccgc cagataccta agtatgctga
     6541 tgaaccgtta taacggtgac cagggtcgtg caatagctgc ctatcatgct ggtatggggc
     6601 atgttgataa ggggagagta gtcgcaggta ccggcgaata tgttactcgt gtcagagggt
     6661 atcagcagat gctcaataat ggtgccgttt atggctctaa ggtagatcat agcgcaccag
     6721 caatttacga aaagatacct gataacgccg ttatcgatca gtctactggc ctggcgttta
     6781 cccctggtga tagcccgttt gagaaaggcg gtctggtcga caaaatcggc aatgctgttg
     6841 gcgttaacga tctggtcaac aaattcatga atggccgggg gatgcgtcgg gaagtcgttc
     6901 agggaacgct agaagaacgt gcacgaggga aggggaccgc aacagcagct ggcaatgtgt
     6961 atgttgatac tccgatgcca gttgaagagg cgcgtccggt ggccaacaac tcaagttact
     7021 ttgaccatct cggcgcacaa atggggattg atggactatt cgataaactc cgcaactcgc
     7081 cggggatgcg gaaaaataat gcgcctgaac cagcctccac gtcccaggtg acgactgccg
     7141 ccaacgattt gcagcaacca accggtcgta tgcagataga cgggcaggtt attagtgacc
     7201 ttggcggttc cggtgccaag ccgacaatgc agttggctga taataccgtt tcacttgatg
     7261 gtgaaacgaa gcggctgttt gcgcagatga cctcattgct tgccaggatt gaagagcaca
     7321 ctaaagactc ggcgaaaggc cagggaactg tcgtaaaggt cagcacgcct cagccgggcg
     7381 ttatgcgcac ggtaccactg tcaattgatg atccgttgat gaatgactac gcgagagttg
     7441 attgatggcc aacaataacg aaattgatcc tttgctgacg ctggagttat ccggcgtaaa
     7501 aacgtatgag tcccaggagg aggcctgggg cgctcgttta tatgagtggc taaacactta
     7561 tcagggtgag gtatacggag atccgtcatg gggcaatgtt ttaccgcagt ttaaacacga
     7621 accgaccaac ttgtcgcatg ttcaaattgc ggttgaggca atgctgttgc aaaaactgac
     7681 ggtagattta cctgacatac cgatttctgg cttgtcagta gccgagggag atgcttttga
     7741 taagttgaaa atatccattc gtatcaggga tataactatc acacaggacg tggtgctatg
     7801 agtaaaacaa caccgactaa agacagtatt cgtgcagagt ttgaagagct tgtcgagaaa
     7861 gattcattct ggtcgaagtt tgtcggctct caatttgtct cgatgctgac attgtttatt
     7921 acccagattg tctacaggtg ctttcagtat gccgatgcgg cgctggctga aggctttata
     7981 tcgaccgcga cgcggcgttc ctctatcctg gcagcggcag aaacgaatag ttacgttggt
     8041 accaagccaa caccgtcatc ggggatgatt gagatcaccg ccacaagtga agatgcccca
     8101 gcggtaatcc ccaaaaacat gcctttaata tctgacgacc agtaccctta catgactatg
     8161 gatgtatgca ggttggttga cggcaccggt acggtagaag tggcacagtt ggaaatccag
     8221 gaggtgacat ataccgttac ggcagccaaa gaatttctgg aagtcgtgtt atcaaaggct
     8281 ctcactgctg tctgctataa gctggaagta ttcgtgacga ccgatggtaa gaccacgcag
     8341 tggtcttcca gcactatgtt ccggttagcc ggtagtaaaa gccaagtcta cgttgagttt
     8401 tataaaccat ccgagcagtt gggggttcga ttcggtgatg ggctaattgg gcaaataccg
     8461 ccagaaggct cgaccattac acttaaggta tggtgcacca acggagatat aaccttggtt
     8521 gctggccaaa atctgacgcc tgtcgattct gcggctaatt tagctaattt gatttcagtt
     8581 aagacaacga cacccataac cgcaggtacc gatgccgaaa caacggagat cacacgtaat
     8641 cgtgcacaat attaccttgc ctatgatgat caggtcgtat ggggcgggga ctatacgtat
     8701 tttctggtgc gtaacatccc gggactgtca tgggtaaagg catggggcga aggccagcaa
     8761 gagaaattag atggtgctta taatgttcgg aatatcaata agatatttat ttcagggtgg
     8821 catccaaata aaagccagtc agagcttgaa gaaatgatcc tggctgcctt taagaaggtg
     8881 ccgaatgagt tgaacaagaa attctcgtat aaagaggtca gaaaactacc atttaagata
     8941 accatcaccg gacggatatc ggcaagcctg accattgaga atgtgaccga tgagctgaag
     9001 tcggcactgg aaacaaaatt tgggcgcgac tcaactttct ttgatccgaa ccgcgtcggg
     9061 aagtacatcc tgattaagaa aaaagacgtt tgggcgttta tcgaaacgct gggttatttc
     9121 cgcgactttt atctggaatt tgtcgagtgg aatgagtcca acggctttta cgatttcgtt
     9181 tatctggata cagaaaactc cacctttaat atttcgtatg aggaggagtg atgcagcgtt
     9241 cctggtttaa taaccggctt acatcagcta agcaaaagtc attgctctat aaatcattgg
     9301 ctgatttggt tcagtcaatg atggacacct ttgttgaccc atggttggag cgaattacca
     9361 accggaagtc tattttctcc atgagcaagg aggatctgga gaccaggaca aatgaacttg
     9421 gccagttctt tactatcaga acctcggact catcttccgt tccgatgttg ttacaacagc
     9481 ggcttgatga gatccacttt aagggaactg aacgtcctat aaaccagaca atttaccgcg
     9541 aatttaacgg tatttctgtt ttatgggatc cgatatatgc accggtggac cttgagcgtc
     9601 atccctatgg cacagttcta ataccagaaa gcacattgga gactaccggc ggcacattcg
     9661 gcgagatgtt tctgacttcc agagggatga tcagtattcc cataaacgac ctggcccgga
     9721 caatgggtat tactggcacg atagatcagt ccgcaattac agaagaaatt ctcagaaagt
     9781 ttaatcagtt cgtaaagcct ctactgccac tgcatatagt gtttgatggg cttacgctct
     9841 atttgtcggt tgttgtaaat gaacaggccg acatgatcac tttgaacgag atttctgata
     9901 ccgaaaaagc attctgctgg tttgaaactt cggatacaac ttcgcttact ggagttacgt
     9961 cgattagcgc cccgatcacc gcaacgcctg gtggcactat tgtgaaagcg acacctacgt
    10021 ttgatcgcac ccgcgcagat gatttgttgc tggatagcga cgcctgacaa tcaccccgtc
    10081 cgcagggcgg ggtgacaagt tacttctctt acaatgaggc ttcacaacag tgattaggga
    10141 aaatcatgtc tgacgtctca acaaacctct ataagagtca gttgttggac tattactatc
    10201 agcggcgcgc tgaatcgtcc attaacaaag gctctcgatt tttaatcagc aaggccgttt
    10261 tcggtaccag ttctctggtt actaagaaag gagatggcac ttatgagatt ggagaactgc
    10321 caaaggcttt cgatctggca gaactgacca gccaattttg taccatcaac ctcgtgccaa
    10381 cctactcagg tgggataatt actgtccgaa tggaccttga tcaaagccag ttgcaggaag
    10441 ggaaaaacta cccattcaac actctggttg ttctggataa cgagaacaag ccaatcgcca
    10501 ttatttgtgt ccaggaagac tcgttgtatg tgggcaaaac atataccgca gttatggcca
    10561 taaacacgac aacagcataa ggatatgctt gatgaatgac gttacagttg ttacatcagt
    10621 tacttaccca tcacccgagt cgttggctct ggtggctgat gtgcaatacc acgaaccata
    10681 tctgtcagcc gcgctaaacc gaaaattcag ggggattgtt gacccgggat tttatgccgg
    10741 tttcttacct aagcctggcg gtgggatgaa cctgttaatc acctctgtgg atggtgataa
    10801 aactgctggc gcagcgtcag tggatattgg tgaattctac caggtaacta ttcagcaacg
    10861 tacggatatt cctcttgcac ttagtgcagg caagaaatat gcaattgtgc tgaagggaag
    10921 atacctcctt ggagaggata cctatcaggt taataccgcg tcacatattc atgcggctga
    10981 atttgttacc agaacctata ccgattcata tcagttagga gatggggagc tgcttgtttg
    11041 tacggtgaat atccctgctg gagtatcagc cattacccag gagatgattg atacatccga
    11101 gcgtattaac cgcacgatcg gcattgatat ttcagactct gtaaccagta gcagaagtga
    11161 tgttgctgca agttcgctgg cagttaaaaa agcctacgat ctggcgaaca gtaagtatac
    11221 ggcgcaggat gcaagcacaa cgcaaaaggg attagttcag ctcagtagcg aaactaacag
    11281 cgacagcgaa acaatggcgg ctacccctaa agccgttaag tctgtaaaag atcttgctga
    11341 taccaaagcg ccaatagaaa gcccgagtct gacaggaacg ccaaccgcgc cgacggcagc
    11401 gcaaggtaca aatagcacgc agatcgcaaa tacagccttt gttaaggcag ctataacggc
    11461 acttatcaac ggtgcacctg gcacactgga tacgcttaaa gaaatagctg ctgcgatcaa
    11521 taacgacccg aatttcagca caactatcaa caatgctctg gctcttaaag ctcctttagc
    11581 aagtcctgca ttaacgggaa tacctactgc gcctaccgct gcacagggta cgaataacac
    11641 gcagattgct acgaccgctt atgtaagagc tgccatatcc gcattggttg gttcatcacc
    11701 agaagctctt gataccctga atgagcttgc cgcagcactt ggtaatgacc cgaactttgc
    11761 gacaacaatg acaaatgcgc tggcaggcaa acagcctctg gatgcaactt taaccgcgct
    11821 tgctggtctt gctacaggcg caaataaatt gccgtacttt accggtacag acactatttc
    11881 tcagactgac ttaacgtcag tcggtcgcga tattctggcc aaaacaagcg tccttgctgt
    11941 tatccaatac cttgatttaa gagaacttgg cacaagcggt gaaaagatcc ccctgttgag
    12001 cacggctaac aaatggagtg cacgccagac tttcaacggt gggatcaccg gggcgctgac
    12061 agggaacgcc gacaccgcga cgaaattaaa aacagccata aacattaatg gcgtcagatt
    12121 cgatggttcg gctgacatta atatcaatac tctggtatct cgcggtcgcg taacggccct
    12181 agaggcgaat gcacagggaa catccgggat tcagctgtat gaggcataca acaatggcta
    12241 cccttccccc tatggcaatg tgcttcacct taaaggtgcc accgctgctg gcgaaggtga
    12301 gttattcatt ggctggagtg gcacgagcgg tgaccatgcg cccgtacata tccgttcgcg
    12361 gcgtgatact gattctgcca actggtctga atgggcgcag gtctatacgt caaaagattc
    12421 aattcccggc gtcaatgcca aaggggatca ggacacctct ggtaatgcgg ctacagcgac
    12481 caagttgcag acagcatgta ctatcaatgg cgtctcgttt gatggttcta ctgatatcac
    12541 tttaaccgct gcgcatgttg ctgcttttgc cagaagagca acggatacgt atgccgatgc
    12601 ggatgggggc gttccctgga atgccgaatc aggcgcttac aatgtcattc gctctgccga
    12661 cagctatatt ctggttaact tctataccgg agtcggaagt tgcccgacct tgcagatgaa
    12721 ggcgcattac agaaatggtg gtctgttcta ccgttcttca agagacggtt atggttttga
    12781 ggaagactgg gcagaagttt atacctcgaa aaatcttcca ccagaaagct acccagtcgg
    12841 cgcaccaatc ccgtggccat cagataccgt tccgtctggt tatgccctga tgcaggggca
    12901 gacttttgac aaatctgcat acccgaaact tgcagcggct tatccgtcag gcgttattcc
    12961 tgatatgcgt ggctggacga ttaagggcaa gcccggcagt ggtcgtgccg tattgtctca
    13021 ggaacaggac ggcattaaat cgcacaccca cagcgccagc gcatccagta cggatttggg
    13081 gacgaaaacc acatcgtcgt ttgattacgg tactaaaacg accagttcat ttgattacgg
    13141 cacaaaaact acgaatagcg ctggaaatca ttcacacaat atacctgttg gtcacactgg
    13201 cgcggggaat ggtgtatcag ccggttttaa cgctgcgtta ggtactggta ccacgtcgag
    13261 cgcaggcggg catgctcaca atgtatatat cggtgcccat aaccacacta tcggcattgg
    13321 tgctcatgcc cattctgtca ttattggtcc ccacggacac accatcaccg ttaacgctac
    13381 gggtaacgaa gaaaacaccg taaaaaacat cgcatttaac tatattgtga ggcttgcata
    13441 atgacattca gaatgagtga acaatcacgg accataaaaa tttataatct actggctgga
    13501 accaatgagt ttattggtga aggtgatgca tacattccac ctcatacagg tctgcctgca
    13561 aacagtaccg atattgcccc gccagatatt ccggctggct tcgtggccgt tttcaacagt
    13621 gatgaggcat cgtggaatct cgttgaagac catcggggaa aaaccgtcta tgacgtggct
    13681 tccggcgacg cgttatttat ttctgaactt ggcccattac cggaaaatgt cacctggtta
    13741 tccccggaag gggagtttca gaagtggaac ggcacagcct gggtgaaaga tgcagaagca
    13801 gaaaaactgt tccggatccg gaaggcggaa gaaacaaaaa acagcctgat gcagatggcc
    13861 agtgagcata ttgcgccact tcaggatgcc gtagatttgg atattgcgac ggaggaagag
    13921 gcatcgttac tgactgcatg gaagacatat cgggtattgt tgaatcgtgt tgatacaaca
    13981 gtagcatcgg atgttgagtg gccagtcgcc ccgcaataaa aggataaagc catcgataga
    14041 aatattgatg gcttcatgta ctctatttat acaatacaac accgctcttt tttgttatat
    14101 atgtgcagtt cgatggtata tctttattta taaaagacat tgcacctatt tttacattat
    14161 ccccaatttt acgtgataat ccaatgatgc aacaattagc tccgatatca acgttactac
    14221 caatttttac tcttgaacca ggcatgtcac catctatctg tccaatggta gtattctgtc
    14281 gtaacaccag attttcacca gcatcaacag caaaatgaac aacaattcca gcatgatggg
    14341 gaattgttaa cccttttcca atatttgcgc ccaatccaat ttcacaacca aatttgttaa
    14401 ttattttact gtttaacttt ttggctgctt tcttatgtaa tttattgcca ttaatataca
    14461 tttcattagc cagtctccac cagaaaagga aattccggtt acgctgtttt ttctctctta
    14521 aaagcctcca gatatccata cgtttccgcc gaattacttc atatttccag aagtttttta
    14581 aattagtaga gtccccaaat aaaacaaagt gaattgccat taagtaagac agcacgataa
    14641 tctccttaat tattatttca gaccacacat attataaggt taagagatta taaaatcctg
    14701 ttgtttgtta ttcaaaaaca attttctgag aaggacatac aacagcaagt cgccagtcac
    14761 cttcatcagg aaattggcga catacgttaa atcagagcag ccccttaact gagctggtcg
    14821 cgctattaag ggatgatgtc accttatctt tgaagccgga caacatatcg ctgaacgatg
    14881 aggattgcag gcgctcccgc aaatcctcat cacagcgttc aagagtcagt gaaaattcta
    14941 tctttttcgc cttaccgtag cgatcaaact cggagcgggt cgtattcgtt ccggtcagga
    15001 catacatgcc gtaaatctgc ccgacaccat caatcagagg ccaggggcgt cctgtatatg
    15061 cctgcgtggt cagcagagac agcgacactt cgccacctgt aatttcagga taaagcacgc
    15121 cggaaagcac gatgcgatca tcacctgcac cgatatactg ccagcttgct gaacggttaa
    15181 cacgttcatt tttcacatgc cgccagcttt tgttttgctg taactgctga tgcggcagtg
    15241 tgcgcagctc aaaaacaaac atgccgtaga tcatcatcat gaccatgact cctcaatctt
    15301 tatcgtaaaa actgccacgt ccggcacggg cgcgccgttc catctctgcc ctgaccattt
    15361 caccgaccag tttcgccagt tcgcggggat tctgcgtaac aacgttatgc agatgaacat
    15421 gaatttcacc gccaaatccg gaggcaacag gctcccggtt acgggaagtt acaggaactg
    15481 atgccactgg agatcgtatg gcctccgcca ccgggcggga gctggccgca acaacaggga
    15541 ccagcgccgg aggcagcgga gccgggacca cgggtgtgat attaattgcg ggggcaggct
    15601 tactgacctg cgcaatcttc cgctcctgcc actccccacg aacagcaagt gcgcggggca
    15661 ggttcttaaa gacaatatcg ccagggccaa tgcgtttttt cgtctcatca accagcttac
    15721 ctgtgttatc agcaattttg ctgagtctgc gtagcgtccc ggtattgctg tctgtgagcg
    15781 gtttgttgtc tttgggttta tcacctccgg tgccattgcc attttccaca ggcttcggcg
    15841 gattgatttt cgccaggtcc ccctgaagta aggcaacctt atcctgaaga atggccgcac
    15901 gctgtgcgtc ttcgattttc ttgcgcgccc tttccgcttc atccggaagg acgccaagtt
    15961 tttcaagtat ccacgccagc gtatccagta gcatttttgc aggtgtcaga acaagttgta
    16021 acgcaccgcc aagaacgtta ccgaatatct cgccagcact ggtacattta tccagcgttt
    16081 ccttgctgga ctccatcggt gacagcagcg atttaaacca gttaaacacc tggctgatcc
    16141 cgcttccgat tgcgtcaaaa acagggccaa accgttcaaa ggtttcacgc aacgggttca
    16201 gcctttccat aatcccgctg aacaccccgg caaaaaatgc cctgatggga tcccagtatt
    16261 tccagataag gacggcagct ccggcaagcg cagccacgat aagaccaacc ggactgaaca
    16321 gcgccccgat agcgcctccc agtaaagaaa cggaacccgt caccattccc catagtgctg
    16381 gcaggaccct gacagcattc attgatccgg tcaggagaga aaaaccaaga cgcagttttg
    16441 ccagcggacc agcaagcaca ccaatagcca gcgacaacga gccaaccgtt gcagtcattg
    16501 ccagcaacgc accgcctgct atcagtagct ggcgcgtcag tgccggatgg gcctgcgcca
    16561 gcgccgtcac ccttgatacc acccgcgtga gccactgcgt gacagaacgc agcggaccgt
    16621 caatcagatc tgcaatgcgg atgcgcaacc cttcccatgc actgccgagt gatttcagat
    16681 cgccgtcaag gttgttggcc ataacctttg ctgtgcgttc agcctcaccg cgcgcgcctt
    16741 caagttcttt tctcagtttg ggtaaggaac cgtcacccgc tgcatcaacg agcgccataa
    16801 acgatgtgaa agcctcttct ccggcaatgt ccttaaagaa cgatacccgg tcaacttccc
    16861 cgtatttgcg ggtggcttta taaaggtcgg ccagcacatc ctccatcggg cgcattttgc
    16921 cgttcgcgtc agagactgcc acaccaagct ctttcagcgc ctctgctgcc gcctttggcg
    16981 gtgatgccag acgagccagg ctggcacgca ttgccgtccc ggcatcactt cccctgatac
    17041 ccatattcgc cagcacgccc gccatcgctg cggcctgctc cagcgatatt cccagcttgc
    17101 ccgccaccgg acctgcatat ttcatggttt cgcccagtgc gcgaaggtca gtgttggtac
    17161 gggtaaacgc tgcggtgagc gtgtcaccga cccggtccat ctggtcggca gaaaggccga
    17221 actgcgtcag gatgtttgag ccaatatccg ccgtctcgcc gagatccata ccgccagccg
    17281 ttgccatgct cagcacaccg ggaagcgcag cctgaatggc ctgtggtgtg aagccagcca
    17341 ttgcaagaaa tgcctggcca ctggcggcat cgcctgcggt gaactgcgtt tcagagccaa
    17401 gttttaacgc ctgctcacgc agcgccttaa actgtgggct gttctggtcg attcgcgtca
    17461 gcgcctgaac gcgggacatc tctttcccga acccgatcgc aggctgcaaa aaacgcccgg
    17521 cagcatagcc gcccgccgct gccgcaccaa ttgccagcgc accacctgtt ttcagttttc
    17581 ccgcggtttc ctgcgcgcgc gaataccgct cacgcgcccg cgttacacgc gcaagcgcct
    17641 gccgttcgcg ttcaagctgg ttgttgtact gttcggtgcg tctgatggcc tgctggatgg
    17701 tgttatcgct gcctgtcagg gaaatgccgt ggcgtttcag ttctccgcca agttcccgca
    17761 ttttctgaat ttcccgtgtg cgcgattcat tcaggcgttc aagccgggtg cttaactgct
    17821 gcatcagctt ttgttgtttt tcgctgagca ctgtacccgt gcgttgtaac tgattaaggg
    17881 cgttaagctg gcgtcgtgct ttcacgatac ccgcatccgc tttactgaca gcgtcgcggg
    17941 cgcgctcaaa tgaacgcgcc tgacgctcga gatttttgat cgccccctgc gttcgctgga
    18001 tggagtcacc aaactgcccc atcaggcggc gggcgttttc ggcaggccgg gtcagcctgt
    18061 caacggcgct gaaagcgacc cggatatcaa gagtcttcat tatctgcatt cccgctgcga
    18121 agtgccgccc gctcgcgcca gctaaccact tcgccgggcg tcatcatgaa gatttcggcg
    18181 ggcgaccagt taaaaatggc ggcaatatcc gccaccagat cttcgatgtg ctcaaagcac
    18241 accagggtga ttacgctgcc gtctcctgca cgctcttcgc gccagagtct ggctcgctca
    18301 taaaatttac agccactgcg cacaactgaa taaaatcgcg tgacgacatt tttttaatca
    18361 tcacttcatc cagtcgtggc gaggtcacgc gaggcaacag cgtaaacatg gtatccgctt
    18421 tcagattcag cacatcagac agcgacagac cacgcaggga tccagcctgc tcaatagccc
    18481 cggtgatctc cacatacgtg attttttcgc caccacgctc aattggtcgg gtcagtttta
    18541 cgccacgttc gacagccata tcctcacctg ccgtcacatc acccgccacg gtgttattcc
    18601 gggtttcagt atcgatgtct ttcatcagtt gtctcctttt cagtcagagg cgacgcactg
    18661 cgccgcctgc atattactta tcagccaagc ccaagcgcgg aacggatacg gtcaggcaca
    18721 atgtccttgc cgtccttccg gtaaatgaag ttcagcaggt caatctccca caacgggcga
    18781 tcgttaacac tcagcttgta gtaggtgttt ttaatggcgt aagtgtgtga tgtggcttcg
    18841 ccctgtttgg cttcccccat atcaatttcc gtcacacgcc cgcgcatctc gatttcatac
    18901 agatcgcttt ctgcatcggt gtagtattca cccacaaaac gcagcagcgt gccgtcaatc
    18961 gtgccgccat acttaaggaa cagcgcacga acagctcccc ccatgacaaa actcgcatca
    19021 agcgcggagt cgtccagacc gagatcaata cttaccgcac ccatcatgcc gcccccccgg
    19081 tagctgtcgg ttttgcgcgt cagtttgggc agagtgacgg acgtcacctt acccacttcg
    19141 ttttcaccat ccacaaacag cgtaaaaaag cgaagatgtt ttggtacagc catcaggcac
    19201 ctcccagcac cgcaaatgcg ggttcaaagt attcatcagt aaacgtctgg taaagctcca
    19261 tgtcttccag tggcggaacg ggcgtatatt tgtagcgaat acgcacacgc ccctgacgta
    19321 aatccgtggt gctgttatcc accacgtcat accagcactc cgcgccaatc agtttcccgg
    19381 cagtaaccag tgaatccagt tttgccctga tggcactgat aacatccttc acgttcgcag
    19441 gcgtcagtgg actgtcgatg gtttcaaact gcgcttccgc aattgaatca gcaagcacct
    19501 gtgcggttcg ggtatacacc tcaaagatgt aggcgttcgt ttccggtgtg cggttgcccc
    19561 agaagcggaa cccgttgcga cgaataatgg tcgtgatttc tttgttgttg aggctgttgg
    19621 catcgctgtc ttcggcctgc aacgaccaga acacatgcct cgacatcccc agcacatttt
    19681 taaccggaac gttggacagt gatttgtgcc agccctgctc atggtcaatg tacgcacgaa
    19741 ggccgcacgc ataggcaggc gcggggaacg tttcgttttt gccacttttc gggttgtagg
    19801 cgatgaagtc cggccataag agcatcacct cacgttcgtt gaatttctgg cggtaggtaa
    19861 tcgcctcagc catcgtgtta cagccgtaac atgaggcata cacaaacgcg cgcagtttac
    19921 ccgcaatcac gcacagggat tttgttaccg cctccgtgtc cagctccggc gcggccagaa
    19981 tacgcggacg gtatccgatg ctttcatcct gctctgcaac aagcagcgca tacatccccg
    20041 tatagctgcc gtcagattca gaaccaccga taaccagttg atcctgcgtt tttccgtctt
    20101 cttctttatg ttcagccacg cgaacgacga tcacctttgt gctcacctgg tctgcgatgg
    20161 ccttaagcgc acgataaagc gtccccgttg ttccgcattt tcccagcacg tcattgacgc
    20221 gggtcagcag tgtgggcttg ttcagcggga acagctccgc gtccgcatca tccgccgttg
    20281 ccacgatacc gataacactg gaatcaacat cattaatcgc tgttaccagg tcggtacttt
    20341 ccgtaacacg ggcaccatga aaacgagttt cactcatagc ttcagcccct tgtatccgtt
    20401 aaatgattcg gcaacaatca tcacccacca cgcgcgtaat ctcacccctg cgccgttctc
    20461 ccgccccggc gacaacaaaa agcagtaacc ccctccgcac gcacatgcga ccatgccgca
    20521 cagggaggga gcagatgacc gacaccacca tgcaattgct cagtcagggc acagaccccg
    20581 tgaaaatgcc ggattttgat attctcgcgg agggtaaaac gctgtcaggc gtggcagagc
    20641 gcctgatgag cctgtcactg accgacaacc ggggatttga ggcggaccag ctcaccatca
    20701 cgctggatga tgcggatggt cagttgcagc taccgccacg gggcgcgcgc ctgacggttc
    20761 tcattggctg gaaaggagaa ccgctgacag aaaaaggcac ttacattgtt gatgaaatcg
    20821 ctcacgaagg accgccggac aggctgactg tttcagccag aagcgcagat tttcgggatg
    20881 aatttaacgt taaacgtgag gtgtcctggc atgatgtgac cgttgagcgt gtggtatccg
    20941 ccatcgctca tcggtatggt ctgaaaccgc aaatcagcga aatgctgatg gatatcgaaa
    21001 tcgaccacgc cgaccagacc gaagaaagcg acatgtcctt ccttacgcgc atggcggaaa
    21061 tgctgggcgc aatcaccacg gtaaaaagag gtaatctgtt attcatcatg cccggcggtg
    21121 gcgtgaacgc acagggccag ccgttgccct cgttcgccat cacacgcagc agcggcgatc
    21181 gccatcagtt ccgcattgct gaccgcgaag cgtatacggg ggtacgcgct tactggcttg
    21241 atcttaatta cgggaaaaag aaaaaagtca gcgtgaaacg ccgcaaaccg tcaaaaccaa
    21301 aaaaggagaa aagcagcagc cgggaaggcg actatatgga aggtgcggaa ggcaacgtgt
    21361 ttgtgttacg caaaacttat cagaacgagc aggcagcaag acgtgcagcg gcggcaaagt
    21421 ggcagcagct acaacgcgga gccgcatcat tctccatcac actggcgcat ggacgtgcag
    21481 aactctaccc cgaaatgcat ggcacggtaa caggatttaa aagcgagatt gataatcagg
    21541 actggattat tgcaaaagcc gagcacacca ttgataacag cggctttacc acgcagcttg
    21601 agcttgaggc aaaaatcccg gaatggatag cagaaacaga gtgagcaact taaatgtatt
    21661 aactcagaac ggatcaacac actcacaaca tagagtcaat atagctgctc gctttgttcc
    21721 agcaaggatg cctatttcta aggaaaaaca gagcagaaac atatggtgtt ttcctacctg
    21781 acatcagggg tggtaagaat catgctttga taaccaattg ataactatta ttatattaat
    21841 ctaacaccat tgacatagtt gaggtttgaa tgaacaaaaa gctgtattta ttcccagact
    21901 caaacatttt tctacaatgc aaggacttga cgcaggtaaa tttttccgag attactgctt
    21961 gtgatgaagt atgtatcatt attacaaggc ccattcagca agagattgac cgccagaaag
    22021 ggcaaggtaa ttcccgccta tccaaaaaag caaggaaggc tgctagccta tttaatcaag
    22081 tagttgattc aactgacatg actcttgtta ttcgtgaacg ctcgccgcga gtttttctta
    22141 ccatggatct aagcctgaaa ccatgtgaac agttatctga acaattggat tatcaggagg
    22201 ccgacgatcg ttttgttggt attgcggcag ggtattgcgc tgacgatcca gattcggagg
    22261 tggcaatcat taccaatgat agcggtccac gtttttcagc aataaaacat aatataatct
    22321 gctataaagt gccttcttca tggttgcttc ctgctgagcc agatgaaaag gataaacaaa
    22381 ttaaattgct ggaagctaag ctgaagcaat tcactcagtc tatgcccgat ttcagcatta
    22441 aagtagccga tgacgagcaa ttaaatattg tacttcctta ttatacagct ttatctgaat
    22501 atgaagttga taatttgctt tgtcaactaa cggatgcatt cccattggtt acaaactttg
    22561 acaatgaaca aactacccca caatcactag aaaaaatcat caagtcattt agtcaacagg
    22621 agtatttccc tgtatcagca gatgatattt ctctttataa agaaaaacat tatccacttt
    22681 ggaaaaagca gtgtgaagaa atactgaaaa actgtcatac tcatattaat ccgaaggcaa
    22741 taaaataccc gctgtcgttt ttattacaaa acacaggtta tactatcgct gaacgtgctg
    22801 tggtaacatt tacagcaaaa ggagattttc gtctgtgtgg atttaattgg gagataatgg
    22861 aagagctgac tgaaagcaaa aggattgacc taccttcagc ccctaccgcc cccaaaggaa
    22921 aatggcgtag tgtatgtggc gtaacagaaa gaatgcctta cgctggggga gctttggaaa
    22981 caccgctatc cagtataatt tcaaaagaaa ttggtccttt gttatctctt actccaacag
    23041 aaagggataa aaacgatttt tattataaag aaaagagaaa tgagccagtt caagttgtat
    23101 cgctggaatg cgaagagtgg cgtcatcaag tcaaagaaga aatattcccg ttgttcgtat
    23161 atacagccaa caaaccaaag aaagtgaatg gtgcaattga ggtgagagtt gatgcaaata
    23221 accttaccaa tccggtcatt aaaacattta aattaaaaat
//
LOCUS       DAZGTR010000070        23020 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGTR010000070 DAZGTR010000000
VERSION     DAZGTR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.070"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            254..1669
                     /gene="tnaA"
                     /locus_tag="ACHLCY_002987"
     CDS             254..1669
                     /gene="tnaA"
                     /locus_tag="ACHLCY_002987"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP0370203.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMSLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1760..3007
                     /gene="tnaB"
                     /locus_tag="ACHLCY_002988"
     CDS             1760..3007
                     /gene="tnaB"
                     /locus_tag="ACHLCY_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP0370204.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3139..4314
                     /gene="mdtL"
                     /locus_tag="ACHLCY_002989"
     CDS             3139..4314
                     /gene="mdtL"
                     /locus_tag="ACHLCY_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP0370205.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4289..5248
                     /gene="yidZ"
                     /locus_tag="ACHLCY_002990"
     CDS             4289..5248
                     /gene="yidZ"
                     /locus_tag="ACHLCY_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP0370206.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5405..6154
                     /locus_tag="ACHLCY_002991"
     CDS             5405..6154
                     /locus_tag="ACHLCY_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP0370207.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6176..6742
                     /gene="yieF"
                     /locus_tag="ACHLCY_002992"
                     /gene_synonym="chrR"
     CDS             6176..6742
                     /gene="yieF"
                     /locus_tag="ACHLCY_002992"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP0370208.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQLRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6796..8133)
                     /gene="adeP"
                     /locus_tag="ACHLCY_002993"
     CDS             complement(6796..8133)
                     /gene="adeP"
                     /locus_tag="ACHLCY_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP0370209.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8300..8965
                     /gene="yieH"
                     /locus_tag="ACHLCY_002994"
     CDS             8300..8965
                     /gene="yieH"
                     /locus_tag="ACHLCY_002994"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP0370210.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9032..9505
                     /locus_tag="ACHLCY_002995"
     CDS             9032..9505
                     /locus_tag="ACHLCY_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370211.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9554..10138
                     /gene="cbrC"
                     /locus_tag="ACHLCY_002996"
     CDS             9554..10138
                     /gene="cbrC"
                     /locus_tag="ACHLCY_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFP0370212.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFTGSFQDDASIEGVEFEYDEEEFAGIKNTYPDEM
                     LKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIR
                     NSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10200..10922)
                     /locus_tag="ACHLCY_002997"
     CDS             complement(10200..10922)
                     /locus_tag="ACHLCY_002997"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0370213.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALD"
     gene            complement(10937..12106)
                     /locus_tag="ACHLCY_002998"
     CDS             complement(10937..12106)
                     /locus_tag="ACHLCY_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0370214.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12133..13749)
                     /gene="bglH"
                     /locus_tag="ACHLCY_002999"
     CDS             complement(12133..13749)
                     /gene="bglH"
                     /locus_tag="ACHLCY_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP0370215.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13835..15229)
                     /gene="bglB"
                     /locus_tag="ACHLCY_003000"
     CDS             complement(13835..15229)
                     /gene="bglB"
                     /locus_tag="ACHLCY_003000"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP0370216.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15248..17124)
                     /gene="bglF"
                     /locus_tag="ACHLCY_003001"
                     /pseudo
     CDS             complement(15248..17124)
                     /gene="bglF"
                     /locus_tag="ACHLCY_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(17258..18094)
                     /gene="bglG"
                     /locus_tag="ACHLCY_003002"
     CDS             complement(17258..18094)
                     /gene="bglG"
                     /locus_tag="ACHLCY_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP0370217.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18380..19105)
                     /gene="phoU"
                     /locus_tag="ACHLCY_003003"
     CDS             complement(18380..19105)
                     /gene="phoU"
                     /locus_tag="ACHLCY_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP0370218.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19120..19893)
                     /gene="pstB"
                     /locus_tag="ACHLCY_003004"
     CDS             complement(19120..19893)
                     /gene="pstB"
                     /locus_tag="ACHLCY_003004"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP0370219.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(19984..20874)
                     /gene="pstA"
                     /locus_tag="ACHLCY_003005"
     CDS             complement(19984..20874)
                     /gene="pstA"
                     /locus_tag="ACHLCY_003005"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP0370220.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20874..21833)
                     /gene="pstC"
                     /locus_tag="ACHLCY_003006"
     CDS             complement(20874..21833)
                     /gene="pstC"
                     /locus_tag="ACHLCY_003006"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP0370221.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21920..22960)
                     /gene="pstS"
                     /locus_tag="ACHLCY_003007"
     CDS             complement(21920..22960)
                     /gene="pstS"
                     /locus_tag="ACHLCY_003007"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP0370222.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
BASE COUNT         5763 a         5863 c         5499 g         5895 t
ORIGIN      
        1 atcgacaata agattgtcga tcaccgccct taatttaccc ttctgtagcc atcaccagag
       61 ccaaaccgat tagattcaat gtgaacgatt agtttgctat atcttaattt tgccttttgc
      121 aaaggccatc tctcgtttat ttacttattt tagtaaatga tggtgcttgt atatatacat
      181 ggcgaattaa tggatattgc agatgtaata ttcacaggga tcactgtaat taaaataaat
      241 gaaggattat gtaatggaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat
      301 tgagccagta aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat
      361 gaacccattc ctgctggata gcgaagatgt gtttatcgat ttactgaccg acagcggcac
      421 cggggcggtg acgcagagta tgcaggccgc gatgatgcgc ggcgacgaag cctacagcgg
      481 cagccgcagc tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac
      541 cattccgact caccagggcc gtggcgcaga gcaaatttat attccggtac tgattaaaaa
      601 acgcgagcag gaaaaaggcc tggatcgcag caaaatggtg gcattctcta actatttctt
      661 tgataccacg cagggccata gccagattaa cggctgtacc gtgcgtaacg tctatatcaa
      721 agaagccttc gatactggcg tgcgttacga ctttaaaggt aactttgacc ttgagggatt
      781 agaacgcggt attgaagaag ttggcccgaa taacgtgccg tatatcgttg caaccatcac
      841 cagtaactct gcaggtggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat
      901 cgcgaagaaa tacgatattc cggtggtaat ggactccgca cgctttgctg aaaacgccta
      961 tttcatcaag cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga
     1021 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtaccgat
     1081 gggcggcttg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
     1141 cctttgcgtg gtgcaggaag ggttcccgac atatggcggc ctggaaggcg gcgcgatgga
     1201 gcgtctggcg gtaggtctgt atgacggcat gagtctcgac tggctggctt atcgtatcgc
     1261 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
     1321 cggtcacgcg gcattcgttg atgccggtaa actgctgccg catattccgg cagaccagtt
     1381 cccggcacag gcgctggcgt gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
     1441 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
     1501 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
     1561 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttaccta
     1621 cgaaccaaaa gtattgcgtc acttcaccgc aaaactgaaa gaagtttaat taatactaca
     1681 gagtggctat aaggatgtta gccactctct taccctacat cctcaataac aaaaatagcc
     1741 ttcctctaaa ggtggcatca tgactgatca agctgaaaaa aagcactctg cattttgggg
     1801 tgttatggtt atagcaggta cagtaattgg tggaggtatg tttgctttac ctgttgatct
     1861 tgccggtgcc tggtttttct ggggtgcctt tatccttatc attgcctggt tttcaatgct
     1921 tcattccggg ttattgttat tagaagcaaa tttaaattat cctgtcggct ccagttttaa
     1981 caccatcacc aaagatttaa tcggtaacac ctggaacatt atcagcggta ttaccgttgc
     2041 cttcgttctc tatatcctca cttatgccta tatctctgct aatggtgcga tcattagtga
     2101 aacgatatca atgaatttgg gctatcacgc taatccacgt attgtcggga tctgcacagc
     2161 cattttcgtt gccagcgtat tgtggataag ttcgttagcc gccagtcgca ttacctcatt
     2221 gttcctcggg ctgaagatta tctcctttgt gatcgtgttt ggttctttct tcttccaggt
     2281 cgattactcc atcctgcgcg atgccaccag caccactgcg ggaacatctt acttcccgta
     2341 tatctttatg gctttgccgg tgtgtctggc gtcatttggt ttccacggca atattcccag
     2401 cctgattatt tgctatggaa aacgcaaaga taagttaatc aaaagcgtgg tatttggttc
     2461 gctgctggcg ctggtgattt atctcttctg gctctattgc acgatgggga atattccgcg
     2521 cgaaagcttt aaggcgataa tctcctcagg cggcaacgtt gattcgctgg tgaaatcgtt
     2581 cctcggcacc aaacagcacg gcattatcga gttttgcctg ctggtgttct ctaacttagc
     2641 tgttgccagt tcgttctttg gtgtcacgct ggggttgttc gattatctgg cggacctgtt
     2701 taagattgat aactcccacg gcgggcgttt caaaaccgtg ctgttaacct tcctgccacc
     2761 tgcgttgttg tatctgatct tcccgaacgg ctttatttac gggatcggcg gtgccgggct
     2821 gtgcgccacc atctgggcgg tcattattcc cgcagtgctt gcaatcaaag ctcgcaagaa
     2881 gtttcccaat cagatgttca cggtctgggg cggcaatctt attccggcga ttgtcattct
     2941 ctttggtata accgtgattt tgtgctggtt cggcaacgtc tttaacgtgt tacctaaatt
     3001 tggctaaatc cttcaagaag ccagccattc gctggcttct tgcctctcag gaaatcactt
     3061 atgtccaaat ggcaactcgc ctgattctcc ttcaccacgt atgctttgcg tcaccttact
     3121 atcaggacgc tttagcccat gtcccgcttt ttaatttgta gttttgccct ggttttactt
     3181 tatcccgccg ggattgatat gtacctcgtt ggtttaccgc gcatcgccgc cgatctcaat
     3241 gccagcgaag cgcagttgca tattgcgttc tccgtttatc tggcgggcat ggcagctgcg
     3301 atgttatttg ccggtaaagt ggccgatcgt tcagggagaa agcctgtcgc catccccggc
     3361 gcggcgctat ttattatcgc ttcggtgttc tgttcactgg ctgaaaccag cacgttattt
     3421 cttgcagggc gctttctaca ggggttgggc gcaggctgtt gttacgtggt ggcgttcgcc
     3481 attttgcgcg acacgctgga tgatcgacgt cgggctaaag tgctgtcgtt actcaacggt
     3541 attacctgca tcattccggt gttagcgccg gtgctcggac acctgattat gcttaaattc
     3601 ccttggcaga gtctgttctg gacgatggca atgatgggca tcgcggtact gatgttatct
     3661 ttgtttattt taaaagaaac gcgcccagcg tcccccgctg cttcggacaa accacgagaa
     3721 aatagcgagt cgctgcttaa tcggtttttc ctcagccgtg ttgttatcac caccctcagc
     3781 gtttcggtga tcctcacttt cgtcaataca tcgccggtat tgctgatgga aatcatgggt
     3841 tttgagcgcg gagaatacgc caccattatg gcgttgaccg ctggcgtcag catgaccgtt
     3901 tcattctcca cgccatttgc gctgggaatt tttaagccac gtacgttgat gatcacctcg
     3961 caggtgttat tccttgcggc ggggatcacc cttgccgttt caccttccca tgcggtttct
     4021 ctgtttggta tcacgctgat ttgcgccggt ttctcggtag gttttggcgt agcgatgagt
     4081 caggcgttag gaccattttc attacgcgcg ggcgtagcca gctcgacctt aggtattgcg
     4141 caggtttgcg gttcgtcact gtggatttgg ctggcagcgg tggttggtat cggcgcatgg
     4201 aatatgctga tcgggattct gattgcctgt agcatagtga gcctgttact gattatgttc
     4261 gtcgcgcctg gacgccccgt tgccgctcat gaagaaatcc atcaccacgc ttgatctcaa
     4321 tctgctgctc tgtctgcaac tgctgatgca ggagcgcagc gtgaccaaag cggcgaagcg
     4381 gatgaacgtg accccttcag cggtgagtaa gtcgctggca aagttaagag cgtggtttga
     4441 cgacccgctc tttgtgaact caccgctggg gctgtcgccc acaccgctaa tggtcagcat
     4501 ggagcaaaat ctggcggaat ggatgcaaat gagcaatctg ctgctggata aaccgcacca
     4561 ccagacaccg cgcggcctga agtttgagtt ggcggcggaa tcgccgctga tgatgatcat
     4621 gcttaatgcg ctgtcgaaac ggatctacca acgttacccg caggcgacca tcaaactacg
     4681 taactgggat tacgattcct tagatgccat tactcgtggt gaagtggata ttggtttttc
     4741 cggtcgcgaa agccatcctc gctcgcggga gctgttaagc tcgctaccgt tagccattga
     4801 ttatgaagtg ctgtttagtg atgtaccctg cgtctggtta cgcaaagatc atccggcact
     4861 gcatgaaacg tggaatctgg acaccttttt acgttatccg catatcagca tttgctggga
     4921 acagagcgac acctgggcgc tggacaatgt gttacaggag ctggggcgcg aacgcacgat
     4981 tgctatgagc ctgccggaat ttgagcagtc actgtttatg gcagcgcaac ccgacaatct
     5041 gctactggcg accgcgccgc gctactgtca gtactacaat caactccatc aactgccgtt
     5101 ggttgctctt cctctcccgt ttgacgaaag ccagcaaaaa aagctggaag ttccttttac
     5161 cctgctgtgg cataaacgga acagccataa tccgaagatc gtctggttac gggaaaccat
     5221 taaaaacctt tacgcgtcga tggcataacc gaatcgtatg aaacgcgacc caatttcaca
     5281 ataaaatgta aaaaagttgt aataagcttg tctgaatcga acttttagcc gctttagtct
     5341 gtccatcatt ccagtaaatg attactctta tattcataat ggaccattaa gcatggagcg
     5401 aaaaatggcg actcactttg cccgagggat tttaacggaa ggacatctga tttctgttcg
     5461 tctcccctcc cagtgtcatc aagaagcccg aaacattccc cctcatcgtc aaagccgttt
     5521 tctggcgtcc agaggtttac tcgcagaact gatgttcatg ctgtatggca ttggcgaatt
     5581 gccggaaatc gtcaccctgc cgaaaggtaa accggttttc agtgataaaa atttgccttc
     5641 gttttctatt tcctatgccg ggaatatggt tggcgtggcg ttaacaaccg aaggtgaatg
     5701 tggcctcgat atggaactac agcgtgcgac gcgcgggttt catagcccac acgcgcccga
     5761 taaccacacc ttttccagca atgaatcgct atggatcagt aaacaaaacg atcctaacga
     5821 agcgcgggcg cagctcatca cgctgcgccg aagcgtgcta aaactaaccg gtgatgtttt
     5881 gaatgacgat ccgcgcgatc tgcagctgct gcccattgcc ggacgcctga aatgtgctca
     5941 tgtaaatcat gtagaagcgt tatgcgacgc ggaggacgtg ctagtgtggt ccgtggcggt
     6001 cacgcccgcg attgaaaagc tcagtgtctg ggagttagat ggcaaacaca gctggaaaag
     6061 cctgccggat attcacagcc gcgccaacaa tcctaccagc cggatgatgc gttttgccca
     6121 gctctctacc gtgaagtctt tttcgccaaa ttgatagaca accacaggag tcattatgtc
     6181 tgaaaaattg caggtggtta cgttactggg gagcctgcgc aaaggctcat ttaatggcat
     6241 ggttgcacgt accctgccga aaattgctcc ggcgagcatg gaagtcaatg cgttaccttc
     6301 cattgccgac attcccctgt atgacgctga cgtacagcag gaagaaggtt ttccagcaac
     6361 ggttgaagct ctggcggaac agctccgtca ggctgacggt gtggtgatcg tcacgccgga
     6421 atataactac tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc
     6481 ggatcaaccg ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg
     6541 cggcgcgcgc tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat
     6601 gaacaagccg gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga
     6661 agtgattgat cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt
     6721 tattcagcga gttaagatct aaataaaaaa cccgccagca atcatgcatg gcgggttttt
     6781 aacgcgctat cgattttagt gagcgtcgat aaagacaatc ttcaggataa acagcagcgc
     6841 aacgatgatt acgcacgggc ttagatcacg cagacgtccg gtaccgattt tcatcacgca
     6901 gtaggagata aagcccagcg cgataccttc ggtaatcgag aagctgaacg gcatcatcac
     6961 ggcggtaata aacgccggaa cagattcagt aagatcctgc cagttcacgc gtgccagact
     7021 ggaagtcatc agcacgccaa cgtaaatcag cgcaccagct gcagcgtagt ctggcaccat
     7081 ccccgccagc ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt
     7141 cagaccggta cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga
     7201 agaagtaccg ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt
     7261 catgcgcggg aatttaccct tctcatccgc caggcctgct ttatcggtca cgccaatcag
     7321 cgtaccggag gagtcaaaca ggttgaccag catgaaagag aaaatcaccc ctgccagccc
     7381 gaggttaaac gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc
     7441 agaaacgatg ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac
     7501 gatagaaacc agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaagaa
     7561 gccgaggata cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc
     7621 cgggttagcg acaatcacac ctgcgttttt cagccccatc atgccaatga acagaccgat
     7681 accgctggta atacccacac gcagactcac aggaatgttg gcaatcatcc agtagcgaac
     7741 gcggaaaatc gtcagtaaca gcagacctat cgcgccccag aagattgcgc ccatcccgac
     7801 ctgccacggc aagcccatcg cctgtacaac gacaaaagcg aagaacgcat tcaggcccat
     7861 agcgggtgcc agtgcaactg gcaggttagc aaacagtccc atcataatac tgccgaatgc
     7921 agcgatcaga caggtagtga cgaagacggc gctggtatcc atgccagcaa cgccaagaat
     7981 ttgcgggtta acaaaaacga tgtaaaccat cgtcaggaag gtggtaaaac cggcgatcac
     8041 ttcggtccgt gccgtcgtgc catgttcgcg cagtttaaac acgcgttcca gcatcccctg
     8101 gccagaagcc tgggttgtgt gttgttgact cattatctat ttccgaacaa ggagggaaaa
     8161 tccgtcgcta tcgtatacca aaatgagaca atatggacgg ctgaagataa ctttttttca
     8221 ttgtatttgc ttatacggca acgattgcgt catgcgaatt ggcatgacga aaacgttaaa
     8281 ctaacgataa aggaaagaca tgtcccagat agaagcggta tttttcgact gcgacggtac
     8341 gctggtcgac agtgaagtca tttgctcccg cgcatatgta acgatgtttc aggaatttgg
     8401 tattacgctc gatcctgaag aggtattcaa acgtttcaaa ggtgtaaaac tgtacgaaat
     8461 tatcgatatt gtttcccttg aacatggtgt tacgttagcg aaaacagaag ctgaacacgt
     8521 ttaccgtgca gaagtcgctc ggctgttcga ttcagaactg gaagccatcg aaggggctgg
     8581 agcgctcctg tcagcgatca ctgcgccaat gtgtgtggta tctaacggcc caaataacaa
     8641 aatgcagcat tctatgggca agctgaatat gttgcactac ttcccggata aactgttcag
     8701 cggctacgat attcagcgct ggaagccaga cccggcgtta atgttccatg cggcaaaagc
     8761 gatgaatgta aatgtagaaa actgcattct ggttgatgac tcagttgccg gtgcgcaatc
     8821 tggtatcgac gcaggtatgg aagtgttcta cttctgcgcc gacccgcaca ataagccgat
     8881 cgttcacccg aaagtcacca cctttaccca tctttcgcag ttaccggaac tgtggaaagc
     8941 gcgtggttgg gatattacgg catagttctt cacactccct tcacttaccc cgcttaaatt
     9001 ggcgctcaaa ggtaagtaaa gggagtttga tatgtctgtt tcacgtcggg taatacatca
     9061 cggactttat tttgcggttt taggaccgtt aattggtgtt ctgtttcttg tcctctacat
     9121 attcttcgca aaagaaccgc tggttctttt gcgaagaata caggtacttc cattgtttct
     9181 cttattgtcg attactacgg gagttattcc tgcattgtta accggcgtga tggtcgcctg
     9241 tctgccggaa aagatcgggt cacagaaaaa ttatcgttgt ctggctggtg gcataggtgg
     9301 cgtcgttatc accgagatct attgtgcagt tattgtacat attaagggca tggcttcctc
     9361 ggagttgtat gaaaacattc tttctggtga cagtctcgtt gtccgcatca ttcctgcatt
     9421 gctggcaggt gtggtgatga gcagaatcat tacccgtctg cccggattgg atatttcatg
     9481 tcctgaaaca gactctttaa gttaagcggg atactttatc tttgggctac tcaaaagcag
     9541 acaggatgtt actatgactc aaaatatcag gccgttaccc caattcaaat atcatcccaa
     9601 gccactggaa acaggcgcat ttgaacagga taaaaccgta gagtgcgatt gctgtgaaca
     9661 acagacgtca gtttattact cgggtccctt ttattgcgtt gatgaagttg aacatctctg
     9721 tccgtggtgt attgcggacg gttctgctgc tgaaaagttt acaggtagtt ttcaggatga
     9781 tgccagcata gaaggtgttg aatttgagta tgatgaagag gaatttgccg gtattaagaa
     9841 cacatatcct gatgaaatgc tgaaagagtt ggttgaacgc acgccaggtt atcatggatg
     9901 gcaacaggaa ttctggcttg cgcattgtgg cgatttctgt gcttttatcg gctatgtggg
     9961 ctggaatgat ataaaagatc gcctcgatga atttgccaac cttgaagaag attgtgagaa
    10021 tttcggtatt agaaattctg atctagctaa atgcctgcaa aagggtggtg attgtcaggg
    10081 ttatctcttc cgctgtctcc actgcggcaa gctgagactg tggggtgatt tttcgtagtt
    10141 atttaaataa tgagaacagg ccggagcgta attcatacat ccggccttat ttcttcagct
    10201 taatcgagcg ctaattctgc ggcagcggct ttatcagcga ttaccatcag cgaaggatgc
    10261 agctgcaaca cggaagctgg aacatcttcg gtaaccggac cttgcagcac atttttcagt
    10321 gcctgcgctt tacctgcgcc actgacaatt atcagcaagt ttttcgccgc catgatgctt
    10381 ttcggcccca tcgtcacgta gctgtcaggc actaacgaaa agtcgccgcc caactcacca
    10441 tgcgccacaa tatcaaccat ctctccctgg atcgggaact ccacggtttg ctcatggaaa
    10501 tgggtcgtat tcggcaaatt gccacaaaaa tgaccatctg cacctaatcc cagcaccacc
    10561 aaatccaggc cgccttcacg cgccagtttc tgatcatgct cgcggtagtt atcaatagtg
    10621 agcttctgga tattctcttc tttgatcccc gcaggggtga aaaacagatt acgcagattg
    10681 gtaatcgtta caccttctcc ctctttgccg cgaaagggaa tttcatcgaa attatagaaa
    10741 tagcagttat cataccaggg cttaccttta accagggtgg tgaggtattc atacatgcct
    10801 ttgggcgtgc taccggcggt aattgccagg ttaacacgac gcgtcttcga catataacca
    10861 agcagatggt gtgccgcgac acggctcatt tcctggtaat cttcggtaat gattaatttc
    10921 atcttagtgc cttattttat ttaaataatt tctgtacgaa ggctgcatag gcaggccgcc
    10981 agacatccat ttcatggttc aggccgggat attcctggta atcaaactta atttttttct
    11041 gctcaagctc agttttcagc ccggcgatat ccttgccggt tacgacatct ttatcaccaa
    11101 cgaccacagt aaaattacgt agttgctggt taatagctgc cggatcgttc agccgggccg
    11161 cgacaccttc atccggtacg gttgtcgtgg taacaccact gaatgtggcc agccagccaa
    11221 agctttccag atgattcatt ccggaaacca gcgcctggta cccgccttgt gaaagccctg
    11281 ccagcgcgcg accatcggca tctttacgga cattaaaacg cttgctaatc agcgggataa
    11341 tatcgttcat cagttcgcga tctgccgctt tagcattcag cggataaaag actttacgtc
    11401 tttcctgagg cacgaaatct tcgggaataa tgcccttcgc atcggtttcg gtatccggga
    11461 tcaccaccag catcggttta attttccctt cagccagcag gttatccatg atttgcggga
    11521 tacgcccctg atcgatagcg gaacgtccgg tatcaccaaa accgtgatag aaatagagca
    11581 ctggcaaagg ctcgcccatg ccggtgtaac ctggcggggt ccagacatac atctgacgtt
    11641 cagattgcaa tgcgttggag tggtaagtta tggcgatcaa atcaccatgc gcaacagaac
    11701 gcgtatccag ataactgcct ggtaccagaa tcatactgga gttaacctgg cgctgaggct
    11761 tagtcattgc ggtgcctgta tcaatgctgc gtacaccatc aacattgaaa aaatactcgt
    11821 acaggttgcc tttcagaata ggtgtgcgcc acgaccagac tcctgcttcg tctttggtca
    11881 tcgggtgaat attgtccgga accggaacac ccaccactac agagacattt tttgccccag
    11941 gggcaaagta gcgaaaggtc acgctgttat cggcattgac ctgagtcaca tactgtttca
    12001 ccgggatagt gggtgccggg ctggctggca tatccgctgc tatggcccac tgagcagaaa
    12061 taccgctggc aatagccagc gttaaagcag caatttttat attcatcgta atattccttt
    12121 cattaagttc cattaccacc agatttcagc ctgggcaccg acagcgaact ggtcgtcttt
    12181 attgtcttcg aaggtgaagc catccagttc gttttccagg gctttgatat acgtggcgta
    12241 gaaacgaatt tccggacgcg aggtcaacat actggtattg actttaaagg tatggaagag
    12301 cgtggtttta taaccagact cattaaattt attactattc gcatctttgt tttgctgggt
    12361 gaaatagccc agttcaacac ctgtctggtt atattggtcc caaatatagg ccgggcgaac
    12421 aaccgcacga atagattcga aatcagagtg tgcgcctgtt tcgtagctat aaatatcgtt
    12481 accgaaggag taaacaatcg cgttagccac aataaaatga tcgccgatat aggcttcgcc
    12541 ctgtgaagtc agacgaactg ctgttccgcc ggtgtgatca ccataataac gaccattaaa
    12601 ggtggtaaat ggacttgcgc cagcataacg gccaaagtta ctggcgatag agttattcgc
    12661 aaccaggaag gagaattcgt tgaagccacc tttatcaaat ttctgcgtta aagatgtgcc
    12721 aaacatccag gtatctttcc agtcataata gccgttatta tcctgattat ctttttccga
    12781 tgcgctttcg tttgccgtga cataacgacc actcaccatt aaggtcgctt tatcccataa
    12841 cgggatatct ttatagcgta aatcaatagt attggtatta atctgctgtt tgttttgcag
    12901 gctgcgatcg taatcatcga catcttcgcg aaccagcgcg atatcaattt tacccggacc
    12961 gactttccag ttttccagac ctacacccgc tgcggcatca gtacgctgcg ttttccagtc
    13021 cagcatctgg atttcaattt tcggcgcacc gtgtttaccc acccagaaat cagcctctgg
    13081 cgcaaagggc aggaaacctt tggtggtaac gtacatatcg gagaactgca taaagttctc
    13141 gccaccggcg ttatcgccaa accagccggt agagtactgc tgaccaacgt taccatccat
    13201 catcacaacg gcatcaaccc gtttgccgtt ttcgttgtag acacgttgtt ttaactgcaa
    13261 atcaaaccag ccggagtatt cgttaccaaa gcggcccaga gaaccaatcg cccatgattt
    13321 aggtgaacca tgagaggcgg tcccccaacc agaacggtaa tagccgttgt aactaaagcc
    13381 aatttcatct ttcacaaact tactgaaatc tttcagagtc atggaagaat aaatcgagcc
    13441 tgtctcactg gcatttttct cttcattttt gaccagtaca gcatcaggtt ttgcggcact
    13501 actggtcggg aacggattgg cggcataccc ttgatcattc gtacttacgc tacgattcac
    13561 cgtcgctggc gtatattttt tcttctcttc atctttatac tttttgagtt cgctttgcgt
    13621 ttctcttaac gctttttcta ataactcaag gcgatgttcc accgttaatg attcagccaa
    13681 tgattgtgca gaaaaggcta acggtgccat taataagatg gcagaggtaa taagatttcg
    13741 tctaaacata atatcccttt atggtgcaaa gaaagaatta acgtatcgca tccagactgt
    13801 tctgaatgcg acgataatta aggtgcttta ttggttattt ttttaatgac agcccccgcg
    13861 ttttgatcac ttctgcatac catccgaagc ttttcttacg cgttcttgtg aggcttcctt
    13921 cgccattatc atcacgatcc acataaataa agccgtagcg cttagacatt tgtgaatgag
    13981 atgcactgac taaatcaatt ggcccccaac tggtgtaccc cataatatcc acaccatcgg
    14041 caatcgcttc atttacctgt accaggtgat cgtttaaata ggcaattcga taatcgtcct
    14101 gtatcgaacc atccgcttca acgctgtctt ttgcgcctaa tccgttctcg acaataaata
    14161 acggtttttg ataacgatcc caaagcgtat ttaacagaac ccgtaatcca accgaatcaa
    14221 tttgccaccc ccactctgaa cttttcagat gcggattggg gatcatattc agtatgttgc
    14281 cctgcgcatt tttattaatg ctttcgtcgt gggaaacaca accagtcatg taataactaa
    14341 aagagatgaa atcgacggta tgttttaaat cttctgcgtc actttcagtc atctcaatgg
    14401 tgatattgtg gtcgcggaag aaacgctgca tatagccggg atactggcca cgcgcctgaa
    14461 catcaccaaa gaacatccag cgccggttct cttccatggc ctgcaacata tcctgtggct
    14521 ggcaggtgag ggggtaaacc agcccaccga gaagcatatt gccgattttc gcttcgggga
    14581 gcaggctatg acaggcttta actgcccgcg cactggcaac cagttgatgg tggatagcct
    14641 gataaacttc cgcctcgcca ctctcttctg ccagccccac gcccgtgaat ggctcgtgta
    14701 acgacatgtt gatttcatta aacgtcagcc ataacgccac tttatgttgg tagcgagtaa
    14761 agaccgtgcg ggcgtaatgc tcgaagtgat cgatgaccgc tcgattagcc caaccgccgt
    14821 agtttttcac cagcccatat ggcatttcgt aatgggataa cgttaccagc ggcttgatcc
    14881 ccgcctgcgc catttcatca aacagccgat cgtaaaacgc taaccccgct tcattcggtt
    14941 cgacttcgtc gccctgaggg aaaattcgcg cccaggcaat ggaaatacgc agacaggtga
    15001 agcccatctc ggcaaataac gcgatatctt ccgggtaacg gtgataaaaa tcgatggcga
    15061 catctttgat attctctttc cccaggatgc gcggttccat ttttcccatt acgccatgag
    15121 gctgtaaatc tgaggtcgag atccctttgc catcttcctg ccaggcacct tccacctgat
    15181 tggcagctgt tgcgccaccc caaagaaatg tttctggaaa tgctttcata attaactcct
    15241 tttatcgtta gcgaatgatg gataacagcg gttcacctgc gcttatctgc gccgtgccgt
    15301 ggggtaatac gtccgtaaaa tcatcgctat tactgattaa taccggcgtc gtcagatcaa
    15361 atccggcctc gcgaatagca gggatatcaa aagaaatcag ccgatcgcct gtattgacct
    15421 tgtcacccac gttgacgtga gcggaaaaga atttgccgtc cagttttacg gtgtcgatac
    15481 cgacatgaat caggagctcc acaccatcat ctgactcaat gccaatggcg tgtaatgtgg
    15541 cgaacaacga agcaattcga cccgcaaccg gagaacgcac ttcaccaacc gagggcagaa
    15601 tggcgatacc tttacccaac aggccactgg caaacgtggt atcagcgacg tgaatgagcg
    15661 gcacaatctc tcccgtcatc ggtgaacaga taccgccctg ctcaggtggt gtaataacct
    15721 ctggtgtttt ctcttgcggg gcaccctgcg ctggctgacg tttagcggtg atgaaatgaa
    15781 gcatcaccgt accgacaaat gcgcaaccga tggcaatgac accgccaata acgctggcct
    15841 agacggtgaa atcaattccc gttgacggga tggtttgcat gaaggtgaaa atacttggca
    15901 aaccaaagga gtacactttc gtttgcgcgt agccaatgat ggtggcccca aagccccact
    15961 gatacaggcg ataacaaagg ggtacttacg cggcagatta acgccatata ccgctggttc
    16021 ggtgatacca aacaggcccg tcaacgccgc tgatcccgcc accacttttt tctgcgcatc
    16081 gcgttcgcag aggaagacgc cgagcgccgc cccgacctgc gccataatgg cgggcattaa
    16141 cagcgggatc atggtgtcgt agcccagcac ggtgaagtta ttgatacaca gcggcaccag
    16201 gccccagtgc agtccgaaca tgacgaagat ttgccagaag ccgcccatta ccgcgcctgc
    16261 aaatgcagga accgcctgat aaagccagag ataaccggcg gcaatcagtt cgcttatcca
    16321 ggttgatagc ggccccacca gcagaaaggt gacgggtgtg ataaccatca gacatagcaa
    16381 tggtgtgaag aaatttttga ttgccgacgg taaccacgca ttaagtcggc gttccagaat
    16441 gctgcacaac caggcagaaa aaataatggg aataaccgat gacgagtaat tcaacaatgt
    16501 gaccggaata cccaggaaat ccagccccag ctcatccgct ttttgcccgt tctcgaaagc
    16561 agtcagaatt aatggatgca ctaacgctcc accaatcacc atggcagtaa atggattacc
    16621 gccgaagcgt ttccccgcgg tgtatcccag gattatcggg aagaaccaaa acaaggcatc
    16681 actggcgctg aataaaatta aataagtacc actttgttcg gtcgtccact gaaaagtgag
    16741 cgccagagcc agcatacctt tcaggatccc ggttgccgcc atcaaaccga tcagaggcgt
    16801 aaaaatacct gaaataacat aaacaaagcg gtttagcaga ttacctttct catcattttc
    16861 cggtgcctgt tgcgcttttt cgccaaggcc tgccacgctg ttgaccgcca ggaagacatc
    16921 ggccacatgg ttacctatga ccacctgaaa ctgaccaccg ctttccacca ccataataat
    16981 accgggggtc tttttcagta cctcagcttg cgctttgctt tcatccttta acttaaaacg
    17041 taatcgcgtt gcgcaatgca tcagactcac aatgttatct gcgcccccga ctcctgcgac
    17101 tatttttctg gctaactccg tcataacttg ccctctaccg ctttgcggca aaactccaaa
    17161 aaaaaacctg aaaaaaacgg cctgacgtga atcaagcaat ttttttcagg ttttgcccgc
    17221 ttagtgcggt aacaatcctt tactcagtaa taatatttca gtgttctttg cgcacgcgct
    17281 ctatatttat ggctaaaaac ataatctctg cgggtgaaat tttacgttga tactgcaaac
    17341 caataaaaat ggcgatccgt tccgcacatt gccatgcttg cgggtaattt tgttttactg
    17401 cttgttgtaa tgattcatca ctatcgttaa ttgaagcatg ttcaagaata cgccaggata
    17461 aaaacttcag atgtgtaacc agtcgctgat aactcaagct ttcttcctgg taattaaggc
    17521 tgaactgaaa ttttattaat tgcagcattt cgcgcattaa ctgcgtgaca cctgcaacat
    17581 cctccatatt tccgctcatt tgggcactga ccagatgcat ggcaataaag cccacttcat
    17641 ctttcggtaa ctgcacgccc aaccgtttat caataatggt taacgcttct tcccctagct
    17701 ggaactcttt cgggtaaagc cgctggatat cccacagcaa cgggttgggc agcaacacgt
    17761 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
    17821 cctgtaattt tcccaagcgc tcctgcgcta aagagataat acgatcacag gttgccatca
    17881 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
    17941 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
    18001 caattcctcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
    18061 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
    18121 aaacccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
    18181 atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat taatttaatt
    18241 gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc gcagttatta
    18301 actttgtgta attttaggaa tttataaaat tatatataac aaatcccaat aattaagtta
    18361 ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt ttatccagct
    18421 catcaccgcc tacatgacgg aaatcctgcc ccttcacgta gtagaagata aactcacaaa
    18481 tattctggca gcggtcgcca atacgttcga tagaacgcgc gcagaacagc gcagtaagta
    18541 cgctcggaat ggtacgcgaa tcttccatca tgtaggtcat cagttgacga acaatacctt
    18601 cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg tcaatgtcca
    18661 tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg cccagcgact
    18721 ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca gtacggcaga
    18781 ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg atcaccataa
    18841 ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg caggcttcat
    18901 cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc ttcgccagat
    18961 cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc accatgccgc
    19021 ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg ccggaaatat
    19081 gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac gaccggtgat
    19141 gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt tgctgaactc
    19201 aatcaattcg cccaggtaca taaacgccgt gtggtcggaa caacgcgcag cctgctgcat
    19261 gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca gctcttcaat
    19321 acgcccggta gagatagggt cgagcgccga acacggttcg tcgagcagca gcacttccgg
    19381 gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag agagagagta
    19441 accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg tcaatgccca
    19501 ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa cgccaaaagc
    19561 gatgttgtcg tagatggaca tcggaaacgg cgtcggtttc tggaacacca tgcccacttt
    19621 cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat caagcagaat
    19681 ttcaccttcc gcacgctgct ccgggtacag ttcaaacatt ttgttgaagg tacgcagcag
    19741 cgtcgattta ccgcagccgg acggcccgat aaacgccgtt acctggtttt tagcgatatc
    19801 caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat tacgaacctg
    19861 aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat cgtgcctgat
    19921 gcacttcgct aatcaggctt accgtaggcc ggatcaggcg ttcacgccgc atccggcaaa
    19981 gtgttaaccg tgtttattct tcgcaaaaac aacgcgcgcc agaatgttca gcagcagtac
    20041 gcacagggta atgatcaata ccccggccca ggccagttgc tgccattccg caaacggact
    20101 catcgcaaac ttaaagatcg tcaccggcag gttggcgatc ggctgcatca tgtccgtgct
    20161 ccagaactgg ttggagagcg cggtaaatag cagcggcgcg gtttcaccag caatacgggc
    20221 aatcgccagc aagataccgg tcatgatccc ggacaccgac gctttcagcg taatcgcgga
    20281 gatcatcttc cacttcggtg tacccagcgc ataagccgct tcacgcaggc tgtccggcac
    20341 cagtttcagc atgttctcgg tggtgcggat aacaatcggc acctgcaaca gcgccagcgc
    20401 aattacgccc gcccagccgg agaagtgctc catctgtgcc accacgatgg tgtagacaaa
    20461 cagaccaacc acaatcgacg gcgcggagag cagaatgtcg ttaatgaagc gaatcacttc
    20521 tgccagccag gatttacgac catattccgc cagataaatc cccgccataa tgcccagcgg
    20581 cgtaccgaat accgtggccc acaaaattaa cagcccgcta cccgccaggg cgttcgccag
    20641 accaccacct tccgtattgg gcggcggcgt catttcagtg aacagcgcca gcgacatacc
    20701 gtcgataccg cgagtgatgg tggacattaa aatccagatc agccagaaca ggccgaaggc
    20761 catcgtcgcc atcgagagcg tcagcgcaat acggtttttg aggcggcgac gcgcctgcat
    20821 tttgcggcga gattcagcca gcgccgcagt ggtttgcatt tcaaccatag ccattagcgt
    20881 gccccctcat tcttagccag gcgcataatc ataaacttcg atgcggcgag gacgatgaag
    20941 gtaatcacaa acaggatcag gcccagttcc atcagtgcgg caacgtgcag accggattcc
    21001 gcttccgcaa attcgttcgc cagcgcagag gtgatactgt tgcccggcat atacagcgag
    21061 gcgctgtcga gctggtaggt gttaccgatg ataaaggtca ccgccatggt ttcaccgagc
    21121 gcgcggccca gccccagcat gatgccgccg ataacaccat ttttggtgaa cggaagaacg
    21181 atacgccaga taacttccca ggtggtgcag ccaataccgt aggccgactc tttcatcatc
    21241 accggggttt gttcgaacac atcacgcatt accgccgcaa tgtacggaat aatcatgatg
    21301 gcgaggatca cgcctgccgc gaggataccg ataccaaatg cggggccaga gaacagcgcg
    21361 ccaacaatcg ggatattcga catgatattg ccgaccggct cctgaaagta aacggcgaac
    21421 agcggcgcaa agataaacag gccccacatg ccgtaaacga tacttggaat ggctgccagc
    21481 agctcaatgg cgatacccag cgggcgtttc agccagccag gcgcaagctc agtcaggaac
    21541 agggcgatac cgaaactcac cgggacggcg atcagcagcg cgataaacga agtcaccaac
    21601 gtaccgtaga tcggcaccag cgccccgtag atatcgttcg gtgcatccca ctctttggtc
    21661 cataggaaag ccagaccaaa tttctgaatg ctcggccagg aggagatgat cagagagaca
    21721 ataatgccac ccaacatcaa tagcacaatc agcgccgcca gttttaccag cacgctgaaa
    21781 attatgtcgc cctttttacc cggtgggtta aaagcaggct tggttgcagc cataagttac
    21841 tcttcagtta aacgcgttta caaagtcatt ttgtaatgcc ggatgcggcg taaaacaccg
    21901 tacccggcct ggagttttat tagtacagcg gcttaccgct actgtcttta atattggtct
    21961 tccacgcagc gcgaacctgt tcaactacac tatccggcag gctggcgtaa tccaggtcgt
    22021 tcgcctgttt agccccggtt ttgtacgccc agtcgaagaa tttcagcact tctgtgcctt
    22081 gttctggttt cttctgatct ttgtggatca gaatgaacgt ggtagaggta ataggccatg
    22141 catcttcgcc tttctggttg gtcagatcct gagcgaaggt tttgctccag tctgcacctt
    22201 ttgctgcatt agcgaagttt tcttcggtcg gactaaccgg tttaccatca gcggagatca
    22261 gtttggtgta cgccaggttg ttctgcttcg cgtaagcata ttcaacataa ccaattgcac
    22321 ccggcagacg ctgaacgaac gcggcgatac cgtcgttacc tttaccgccc agaccgatcg
    22381 gccattttac ggtagagcca gtaccaacgt tgtttttcca ctcttcgttc actttcgcca
    22441 ggtagctggt gaagacgaag gaagtcccgg agccatctgc gcggcgtact acagcaatgt
    22501 tttgtgaagg cagtttcaga cccggattca gtttggcgat ggcttcatca tcccacttct
    22561 tgattttgcc caggtagatg tcgccgaggg ttttaccatc cagcaccagt tcgccagact
    22621 tcagccctgg aatgttaacc gccagcacca cgccgccaat cacggtcggg aactggaaca
    22681 gaccttcctg agccagtttt tcgtcagaca gcggcgcgtc agaggcacca aaatcaacgg
    22741 tattagcgat aatctgtttt acgccaccgg aagaaccgat accctggtag ttaactttat
    22801 taccggtttc tttctggtaa gtgtcagccc atttggcata caccggcgca gggaaggttg
    22861 cacctgcacc tgtcaggctt gcttctgcaa acacagagaa agcgctcatc gataaggtcg
    22921 cggcgacaac agttgcgacg gtggtacgca taactttcat aatgtctcct gggaggattc
    22981 ataaagcatt gtttgttggc tacgagaagc aaaataggac
//
LOCUS       DAZGTR010000071        22048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGTR010000071 DAZGTR010000000
VERSION     DAZGTR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.071"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..614)
                     /locus_tag="ACHLCY_003008"
     CDS             complement(<1..614)
                     /locus_tag="ACHLCY_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0370223.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPP"
     gene            complement(817..1218)
                     /gene="nikR"
                     /locus_tag="ACHLCY_003009"
     CDS             complement(817..1218)
                     /gene="nikR"
                     /locus_tag="ACHLCY_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP0370224.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(1224..2030)
                     /gene="nikE"
                     /locus_tag="ACHLCY_003010"
     CDS             complement(1224..2030)
                     /gene="nikE"
                     /locus_tag="ACHLCY_003010"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP0370225.1"
                     /translation="MTLLNISGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(2027..2791)
                     /gene="nikD"
                     /locus_tag="ACHLCY_003011"
     CDS             complement(2027..2791)
                     /gene="nikD"
                     /locus_tag="ACHLCY_003011"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP0370226.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSDGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2791..3624)
                     /gene="nikC"
                     /locus_tag="ACHLCY_003012"
     CDS             complement(2791..3624)
                     /gene="nikC"
                     /locus_tag="ACHLCY_003012"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP0370227.1"
                     /translation="MNFFLSSHWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3621..4565)
                     /gene="nikB"
                     /locus_tag="ACHLCY_003013"
     CDS             complement(3621..4565)
                     /gene="nikB"
                     /locus_tag="ACHLCY_003013"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP0370228.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4565..6139)
                     /gene="nikA"
                     /locus_tag="ACHLCY_003014"
     CDS             complement(4565..6139)
                     /gene="nikA"
                     /locus_tag="ACHLCY_003014"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0370229.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(6250..6837)
                     /gene="acpT"
                     /locus_tag="ACHLCY_003015"
     CDS             complement(6250..6837)
                     /gene="acpT"
                     /locus_tag="ACHLCY_003015"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP0370230.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWH
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6892..7941)
                     /locus_tag="ACHLCY_003016"
     CDS             complement(6892..7941)
                     /locus_tag="ACHLCY_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0370231.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            8073..9290
                     /locus_tag="ACHLCY_003017"
     CDS             8073..9290
                     /locus_tag="ACHLCY_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0370232.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(9294..9851)
                     /gene="dcrB"
                     /locus_tag="ACHLCY_003018"
     CDS             complement(9294..9851)
                     /gene="dcrB"
                     /locus_tag="ACHLCY_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP0370233.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9924..10589)
                     /locus_tag="ACHLCY_003019"
     CDS             complement(9924..10589)
                     /locus_tag="ACHLCY_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP0370234.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVLTSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10810..11055
                     /gene="tusA"
                     /locus_tag="ACHLCY_003020"
     CDS             10810..11055
                     /gene="tusA"
                     /locus_tag="ACHLCY_003020"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP0370235.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGRPYRYLIRKGG"
     gene            complement(11157..13355)
                     /gene="zntA"
                     /locus_tag="ACHLCY_003021"
     CDS             complement(11157..13355)
                     /gene="zntA"
                     /locus_tag="ACHLCY_003021"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP0370236.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQSADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13429..14055)
                     /locus_tag="ACHLCY_003022"
     CDS             complement(13429..14055)
                     /locus_tag="ACHLCY_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP0370237.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            14196..14555
                     /locus_tag="ACHLCY_003023"
     CDS             14196..14555
                     /locus_tag="ACHLCY_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP0370238.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14558..14827)
                     /locus_tag="ACHLCY_003024"
     CDS             complement(14558..14827)
                     /locus_tag="ACHLCY_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP0370239.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14817..15413)
                     /gene="rsmD"
                     /locus_tag="ACHLCY_003025"
     CDS             complement(14817..15413)
                     /gene="rsmD"
                     /locus_tag="ACHLCY_003025"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP0370240.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15563..17059
                     /gene="ftsY"
                     /locus_tag="ACHLCY_003026"
     CDS             15563..17059
                     /gene="ftsY"
                     /locus_tag="ACHLCY_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP0370241.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            17062..17730
                     /gene="ftsE"
                     /locus_tag="ACHLCY_003027"
     CDS             17062..17730
                     /gene="ftsE"
                     /locus_tag="ACHLCY_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP0370242.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17723..18781
                     /gene="ftsX"
                     /locus_tag="ACHLCY_003028"
     CDS             17723..18781
                     /gene="ftsX"
                     /locus_tag="ACHLCY_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP0370243.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRARITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILMLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            19026..19880
                     /gene="rpoH"
                     /locus_tag="ACHLCY_003029"
     CDS             19026..19880
                     /gene="rpoH"
                     /locus_tag="ACHLCY_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP0370244.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            20151..21254
                     /gene="livJ"
                     /locus_tag="ACHLCY_003030"
     CDS             20151..21254
                     /gene="livJ"
                     /locus_tag="ACHLCY_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP0370245.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGMATDAK"
     gene            complement(21453..21836)
                     /gene="panM"
                     /locus_tag="ACHLCY_003031"
     CDS             complement(21453..21836)
                     /gene="panM"
                     /locus_tag="ACHLCY_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP0370246.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNLGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5233 a         5831 c         6100 g         4884 t
ORIGIN      
        1 ggcggcagcg gcgcaggcag cacctcatcc gcttccggca cccgctcaca ccagccgagc
       61 agccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact caggcgaagc
      121 tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccaca
      181 ccgccgcgca ccagccagag tgattcgctg cggctgtaac cgtcctcacc gggaaacagg
      241 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      301 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      361 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg
      421 gcgatgtcgg tttcaccggg aaggaccttt gcacccagca gcggattgac cggatggccg
      481 gacgtcaccc cgccggggca caccgaacag gccacgccgg tgggggcacc aatacgtacc
      541 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcaccctg acgcgccgcc
      601 ggttttccgc tcatcgtaaa atccttttct attattttaa tgacagtatc ttcatacgaa
      661 aaagcgaaga aaaacttgtg gtcacaatga attgaagatt tttaggaata tatttagaac
      721 gttacaaaag agaaggttcg gatagggtat ttagagagga atcaggtgtg ctagtggccc
      781 tttgttgatc gttcaccaaa ggacccaata atagactcaa tcttccttcg gcaagcactg
      841 caaatgcccg tgccgcacgc cgcgctgggc gataacgtca tcggcaaaat gctgcacgtc
      901 gcccatatca cctttcagca cggcgatttc cagacagtcg tcgtgattga tatgcacatg
      961 cagcgtggcg acggagagat cgtggtgatg gtgctgggtg gagacaatgc ggctggctaa
     1021 gtcgcgtttt tcgtgttcat acacatagga gagcaccgcg aaaccttgcg tgccgtgctg
     1081 ctgagtggcc tcctgggcca gagcgctacg cagaatatcg cggatagctt cggaacgatt
     1141 gttataacca cgacgctggc tcaggctgtc cagcgtctcc agtaaatcgt catcaagcgt
     1201 gatggtgact cgttgcattt gcgttaaacc ttttctgtgg tgcgacggcg cacggggaat
     1261 gcgggtaata ccgcgttttg tagcacacgt ccggcgtcag aggaaaaggt taatttatct
     1321 cccaccacct gggtttcgac gatttgtccg ttgtccatca ccatcacccg ctgacagaag
     1381 cgttccacca ggcgtaagtc gtgggtgatg aacaggcagg cggtgccaaa ctgttgttgt
     1441 agctttttca gcaggcgaat gacgcccgcc tgtaacacga gatcaaggtt agaaacggct
     1501 tcatccagaa tcagtagttt cggttcgacc gccagcgcgc gagccaggca gacgcgctgg
     1561 agctggccgc cgcttaactg cggtgggcgt ttgtcgagaa cgctgtcatc gagatcgacc
     1621 gccttcagca tttcgctggc gcgcgccagt tgttcggatt ttttcagtga tagcaggtgg
     1681 cgcatcggtt cacgcaggat ctcgcgcacg gttttgcgcg gattcacggc gctgatggag
     1741 tcctgaaata ccatctgaat atcgcggcgg aacgctttac gctgggcgcg attgagtttc
     1801 gccagcggtt cgccacgcca gctaatattc ccctgcgcgg gcgattctaa acccaccagc
     1861 aaccgcgcga gggtactttt cccgcagccg ctgcgcccca gcagggcgac ggtttcgccg
     1921 cttttcaggg tcagggaaac gttattcagc accgcctgat gttgatgttt tccgttaaat
     1981 ccaccgtgcg catagtgatg ggaaaggccg gagatgttaa gtaaagtcat gatgccagct
     2041 ccataccgta gagggcgaga tgagcggaaa ccaggctgcg cgtcactgta tgtttggggg
     2101 cgttaaacag cgtttctaca tcgccctgtt cgacaatctt accgtcagac atcaccgcca
     2161 cgtcatccgc cagacgcgcc acaacgccca tatcatgggt caccagcagc attcccggcg
     2221 cttgtttttg cataatgctt tccagcagat cgaggatgcg cgcctgtgct accacgtcga
     2281 ggtcggtggt cggttcatcg gcgatgataa acggtgattc acacagcacc gccatcgcaa
     2341 tcatcatgcg ctgcaacatg ccgccgctca tctcgaacgg gtacagcttc agcacgcgcg
     2401 cggcgttttc cagccccacc gcttctatgg cagcggtaag cgtagcgtca tcggcgggtt
     2461 tccctaacgc caggcaggtt tcccgcgcgt gggtgtgcat ggtgtgcagt ggattaaagg
     2521 cgctgcgcgg gttctgcatg atggtggcaa ttttgatgcc gcgcagggcg caaggcgaaa
     2581 ccggtttgcc atcggctaaa atttcccccg ccgtctggcg aacgccagcg ggcaaaatgc
     2641 ccagcgtcgc ggcgcaggtt aatgatttcc cgctgccgct accgccgact aacgccagca
     2701 cgcgcccgcg ttgcagggtt aacgatacac cgtgtaccag cggctgcgcg gcctgtagcg
     2761 cgatattacg tagttcaatc tgttgcggca ttagtgtgcg tgctccgtca ccagatgagg
     2821 gtccagatga tcgcgcagtg cgtcacccac caggttaaag gccatcacgc tgataaacag
     2881 cgccagcccc ggccagaaca tttgcagcgg ctgggtccag atatactggc gcgcgtcgtt
     2941 aatcatcacg ccccattcgg cggtcggcgc ggtcacaccg aggccaagga aagacatccc
     3001 cgcgacgtgc agcatcatat ggccgatatc cagcgttgcc agcaccagca gcgaagggat
     3061 caccgcgcct gccagatgat cgacaaacac ccgcacatgg cccgcaccgg aaagccgtga
     3121 cgccagcaca aactcgcgtt ggcgtagtga aatcaccagg ctgcgcacca tgcgtgcata
     3181 ccacgcccag tgcgacaggg cgatggcgat aattacgttg gtcagcccgg tgccgagcac
     3241 gccaaccata aagaacgaca gaatcgaggt cgggaaggtc ataaacatat cggcgacgcg
     3301 catggtggcc tgatcaacgc gcccgccaat caacccggcg ctgccgccaa taaccagccc
     3361 taatgtcagc accagcagca ggcaggccat taccgaaccg agcgacacgc gggtcgctgt
     3421 catcagccgc gagaaaatat cgcgacctaa gtgatcggtg cccagccagt gctgcgcatc
     3481 cggcgaaagc aggcgcgacg gcaaatcaat cgcctggggg tcatacggca gccaccactg
     3541 gctggtgagc gcaatcagcg ccagcagggc gataatgatc agcgccaggc gtaccgacca
     3601 gtgggaagag aggaaaaagt tcacgcgtgc gctccttcat gacgacgaat gcgcgggtcc
     3661 agcgcggcgt tgagcaaatc gacaatcaaa ttacagacca caaaaaccac caccatcatc
     3721 agcgtaaagc actggatcac cggatagtca cggttaaaaa tcgccgacac cgcatagcgc
     3781 ccgacgcccg gccaggcaaa gatgttttcg ataatcatcg tcccgccaat cagttcgccg
     3841 atatgcatcc ccacggcggt gatcatcggc agcgaggcat tgcgcaggat gtggcgacgt
     3901 tcggtctgtt tgtcgttcag gccgcgcaga cgcgcccagg tgacgtgacg ctgaccggcg
     3961 acgtccagca tactggcgcg cagtaaacgc gcgttaatcg ccagcgacat aaaggcaatg
     4021 gaaaccgcag gcaaaatgat gtgctgccag ccgccgtaac ccatcgcggg tagccattgc
     4081 agatacaccg aaaacgccat caccagtaaa aacgccagcc agaagttagg catcgacacg
     4141 ccaagaaacg cgatgaaacg tacggcgaaa tccggcagac ggtcgcgatg gcgcgccgcc
     4201 cagataccga gcggtacgga agtgagcaga attaatacca gcgccgcacc tgcaagttcc
     4261 agcgtggcgg gcaggaagtt cagcatatcg tccagtaccg ggcgttggct ggcgaatgag
     4321 ataccaaagt caagatgcag cgccttccac aaccaggtac cgtactggac gtacagcggc
     4381 tgatccagcc cgagcatggt gcgggtagag gccagcatct ccggcgtcgg cggcaggtta
     4441 gataaacgca gataatcgag cgccgggtcg ccggtaccga ggcgcagcat cagaaaaatg
     4501 atcaccgagg cggcgagcac catcgggatc agcagcagaa agcggcgtaa tacgtaacgc
     4561 aacattaagg tttcaccggt ttaatctgtt cgaacggaat ttcggtggcg atcggcgcgt
     4621 aggggatgtt acccagctcc ggttttgata ccaccatcat tgagatgtaa ctgataggca
     4681 gataaaccgc ctcgtcatgc agacgggtca gaatgtcgcg atacagcgcc tgacgttgcg
     4741 tttcgtcatg ggtcgccagc acttcgccga tctctttatc aatcagcggt ttgtcggcta
     4801 atccttgctg tgcctggaag tcagcgtgtg acggtacgcg cattgaactg aggaaggcgt
     4861 gtggatcata tggcgcgccc caggtgcggt ggaaaatcat gccaaaacga ccgtcgcgct
     4921 gacgagcata gatactgctc tcttcttcgc caatcagcga gacatctgcg ccaatctggc
     4981 gcatatcagc ctgaatgatt tccgccatcg atttgcttaa cgcatcggtg ccgatgaacg
     5041 acagttcaat gcgcagcggc tgaccatttt tctcgcggat gtctttgccc gcaggcagcg
     5101 tccaaccggc tttttccagc aacgctttcg ctttctgcgg atcgtactgg cgcggtttca
     5161 ggccgaggtt ggcgtagggc acagaagggg caaacagggt gtcggcgacc tgctgggtgc
     5221 catacaacgc gttatcaatc agcgattttt tgtttaccgc gtaattaaga gcttcacgta
     5281 ctgccagctc gttggtgggg gctttggcgg tattgagcgc cagcatcacg gtttcgatcg
     5341 gctgtgacag ttgggtgtgg taagccggat tctggctaaa gcgggcgaag gtatcgagcg
     5401 gtaataaccc ttcgtttccg tacagcaggt cgatatcgcc agtttcaaac gccaccgcgc
     5461 gggtagtcgg gtccgggatg acgttaaagg tgatcttttt aatcgctggc ttttcgcccc
     5521 agtagttttc gttacggacg aagacatcgt actgattcag tttcgattcc tgcaaaatcc
     5581 acggtccggt gccaatcggc gctttaattc cgttcatggt ttcatggttt ttaaactgcg
     5641 agggagcgat aaaacggaaa ggacggggca gggccagttc ttgcaggaaa ggatagtagg
     5701 cgcttttcag ggtaatttgc agctctgttt tactgagtgc tttaacatca acaatctggt
     5761 ttgccagctc cagccaggcg tgacgttgac ggttatcgag cactgcgcgg aagttttctg
     5821 ccgccgcctc ggcatcgaac ggttcaccgt tggagaattt cacgtcatca cgcagggtga
     5881 aggtccaggt tttaccatct tctgaatgag tccagctttt tgccagccac gggatcaccg
     5941 aaccgtctgc ctgatatttc accaatggtt cataaaccat gctctgggcg aacatctggt
     6001 taggcgtgta aaggtgcggg tttagtggcc cgacattcac cggccaggcg gtggtgattt
     6061 catctggtgc agcggcatgg acgataaaag acgcacaagc cagcagcgca aatagagtgc
     6121 ggcggagtgt ggagagcatg gtaaccccaa tggattaaaa tagatggcgg aaataagtat
     6181 gacgatttta agtattcgtc atactgatta cctgttctgg atcaatagat gggcagtcgg
     6241 gtgggcggat cagttaactg aatcgatcca ttgcacactg tcggcggtga gcgtaaaggg
     6301 agtaggggtg cagatcgcca ggcttaaatt ttcaagctga caatggctga ctgacagcga
     6361 ggagtgatag gtgctgtcta cgctgacgat ttgccaggcg ctgccgccac gctgtttaac
     6421 gatggcttct ttgcgcgtcc agatgcgcca gaacatttcc agttgctgat caggatgcac
     6481 ggcgtccatc tcagcgtgtt ccccgaggct gaatacggcg ttcgccagcc agtgccagtt
     6541 ggcgcgcggg cgaatcactt cgatatcgca gccgacttcg ccttcatcac tcaacagcag
     6601 ggcgatatcg tcaccgctat ggcttaagtt gaaccatagc ggcatttccg gcgcaaatgc
     6661 aggtttgcct tgttcgccat agatgatctc cggtagcggg gaaagcgtgt gcgaaagcaa
     6721 tgcacgcccc gccagccagc gttcgcgtcg tggaccttgc ggtgcttgct cgcgtaaacc
     6781 cggtggcagt ggagctgcgc ttaaggtcga aactttcccc agaactatcc gatacatatc
     6841 agggccaacg tttaatggaa aatgaaagtg cgtatcgtat cacttgtcgc ctcatcccgg
     6901 taaccgactt ttcggtctgc ctggccccag taaaatcgcc agtttgctac cgcctttggt
     6961 ggtttccatc cagattttac acacactggt taatggcacc gaaagtagca tccctaccgg
     7021 gccgagcagc catccccaaa ttaacaatga aagaaatacc accatggtgg acatccccag
     7081 gcgatggccc atcatccgtg gttctaaaat attgccgatg accatatgga ccactaaaaa
     7141 caatgcgccg accagaatac attcgtaaac accattaaac agcagcacct gaatcattgg
     7201 cggtacggcg gaaattaccg cgccgatatt gggcacgtag ttgagcaaaa acgccagtac
     7261 tgcccacatc agcgcaaact gcacccccat cagctccagc cccagccaga cgatgacacc
     7321 tgtccataaa ctgagtagcg tcttcaatgc aagatagtgc gaaacgcctt taagtgcgcg
     7381 gtgtaatcct gcgatgtgaa tctgtgggtt attcagcgca aaacgcattt tgtaagggac
     7441 gtggcgcact tcaaacagca taaaaactac ggtcatcacc agcaaaagca cgctcgccat
     7501 tgccccggaa agcccggtca ttagcgctgt ggtgaaggta accacttttt ccgagtccat
     7561 ccgctgcagc attcgctccg gcgacatatg caaattaaga aaaggcaaca tctcctgcaa
     7621 tttaaaaagt ttgcgcgtca actccttatt aaacttcggc agcatagaga taaattcgtt
     7681 aaacgatgcc gccagtacgc cgaccagcgc agttagtgcg atcagcatca ccaccaccac
     7741 aatcgtaatg gcaatggggc gttgtactcc ccgacgaata aaccaggtga cgagcgggtt
     7801 gaggacgatg gcaaaaaaca gcgccagtaa cagctgcaca atgatatctg ccgctgcgtg
     7861 aatgcccgcg aggatcacta ccagcgaggc cagcttgagc agaatgtgca tgcccgtttt
     7921 atcgggttga ggggtttcca ttggggcttc cttgtgactt tttgtattaa gtgtagcggg
     7981 agtcacgcca gcaatattct gattcttact gctgattttc acgtcagcac gtggtaaaaa
     8041 tgaaacactg ttgtaaaaat gtggtgatcc tcatgcccga acccgtagcc gaacccgcgc
     8101 taaacggatt gcgcctgaat ttgcgcattg tctccattgt catgtttaac ttcgccagct
     8161 acctcaccat cgggttgccg ctcgctgtat taccgggcta tgtccatgat gtgatgggct
     8221 ttagcgcttt ctgggcagga ttggttatca gcctgcaata tttcgccacc ttgctgagcc
     8281 gtcctcatgc cggacgttac gccgatttgc tgggccccaa aaagattgtc gtcttcggtt
     8341 tatgcggctg ctttttgagc ggtctgggat atctgacggc aggattaacc gccagtctgc
     8401 ccgtcatcag cctgttatta ctttgcctgg gacgcgtgat ccttgggatt gggcaaagtt
     8461 ttgccggaac gggatcgacc ctgtggggcg ttggcgtggt tggctcgctg catatcgggc
     8521 gagtgatttc gtggaacggc attgtcactt acggggcgat ggcgatgggt gcgccgttag
     8581 gcgtcgtgtt ttatcactgg ggcggcttgc aggcgttagc gttaatcatt atgggcgtgg
     8641 cgctggtggc cattttgttg gcgatcccgc gtccgacggt aaaagccagt aaaggcaaac
     8701 cgctgccgtt tcgcgcggtg cttgggcgcg tctggctgta cggtatggcg ctggcactgg
     8761 cttccgccgg atttggcgtc atcgccacct ttatcacgct gttttatgac gctaaaggtt
     8821 gggacggtgc ggctttcgcg ctgacgctgt ttagctgtgc gtttgtcggt acgcgtttgt
     8881 tattccctaa cggcattaac cgtatcggtg gcttaaacgt agcgatgatt tgctttagcg
     8941 ttgagataat cggcctgcta ctggttggcg tggcgactat gccgtggatg gcgaaaatcg
     9001 gcgtcttact ggcgggggcc gggttttcgc tggtgttccc ggcattgggt gtagtggcgg
     9061 taaaagcggt tccgcagcaa aatcaggggg cggcgctggc aacttacacc gtatttatgg
     9121 atttatcgct tggcgtgact ggaccactgg ctgggctggt gatgagctgg gcgggcgtac
     9181 cggtgattta tctggcggcg gcgggactgg tcacaatcgc gttattactg acgtggcgat
     9241 taaaaaaacg gcctccggaa cacgtccctg aggccgcctc atcatcttaa aacttactga
     9301 ataaccagcg tattaatgat gttttctgcg gtggtctgcg ctttttgctg atcgtcagct
     9361 ggcagagtaa tttgcatggt cagcagttga ttacccacgt tacccagaat aacggaagag
     9421 tacgccgtct ggcctttcgc ggagataata ctgtctaact gctgcatttt gtgacctttc
     9481 agctcaatgg ctttattggt taccacttgc agctgcggat cgcggctacg ttgctgatct
     9541 tccagacgct tcgccagcac cgccagatct tcttttggat catcgcccat gatgacgatg
     9601 actgctttct gcccggtggc gtcggaccag acatgcatgt tattggcctg cgttcccagc
     9661 ttaccgctct ggtcggtcat atccgctggc agcgagaaac ttaacttgcc atcaagcagg
     9721 ttgacgggat tcccggtagc gttactttcc gcgaccgaac cctgcgccgt agcgttagtg
     9781 tctttatcat cacaggccgc aagccccata accagcaggc caattccgac atatttaacc
     9841 agattgcgca ttgacttctt cctttcgata aacggccata acggctcatt catccatctt
     9901 atcacaactc tgataacgaa cctttaactc gcctgcaaag cgttgatttc ggatttatct
     9961 gccagtcttt tcaacagcat attgagtaat acgccataca ttggcaggaa gaaaacgata
    10021 ctgattaaca ctttgaaaca gtaatcgacc agcgcgattt ccatccagtg ttcagccata
    10081 aaggcatccg ggctacgcca gaaggcaatg aagaaaaagg ccagcgtgtc gctgacgtta
    10141 ccgaacagtg tggacgctgt cggtgccagc caccagcggc gactttgacg caggcggtta
    10201 aaaacgtgca cgtcgaggat ttgccccagc gcgtaggcca tgaaactggc ggtggcgata
    10261 cgggcgacaa acaggttgaa gtgggcgagt gcgccgaatc cctgccatga acccatatag
    10321 aacagcgacg agatgacata ggagattaat aacgcaggga tcattaccgc gaagataatg
    10381 cgtcgggcca gcggtgcgcc aaaaatacgc acggtcaggt cggtagcaag aaaaataaac
    10441 ggaaagctaa acgcgcccca ggtggtatgg aaaccaaaaa tggagacggg aagctgaacc
    10501 agatagttac tggaggtgag caccagcaga tgaaataacg ataaccagaa caacgcctta
    10561 tagcgttgag tttgcgagaa aacgttcata ttgtaccttt ttgattaacc attggggtga
    10621 gggaacccaa tacgtacgac acgtctgtta ccttaacata ataacgtgct tacccttttt
    10681 tcgagccgcc gcatgatact gctttacgtt gacaatgcaa tggttaattt tcacgcaatc
    10741 gttaacctgg tttgcttacg gactcacagg gcgtaaacta gcgccgtttt tttatgtgat
    10801 gagaagaaaa tgaccgatct cttttccagc cctgaccaca cactcgacgc acttggcctg
    10861 cgctgcccgg aacccgtaat gatggtgcgc aaaactgtgc gcaatatgca gcccggcgaa
    10921 acgttgctga ttatcgccga cgatccggcc actacccgcg atattcctgg gttttgtacc
    10981 tttatggaac acgaactggt tgcgaaagag acggatggac ggccttatcg ttatttgatt
    11041 cgtaaaggcg gttgatgggg gctgattggc ttcgatgccg ccttttcccc tcaccctaac
    11101 cctctcccca gaggggcgag gggaccgatc gcgctcaatg ttgcgatcgg tttgccttat
    11161 ctcctgcgca acaatcttaa cgcattcgct gtcaccagca ccgtcgcacc cgtatctgcc
    11221 agcaccgcca gccatagtcc ggtcatccct aacagcgtgg tgacgaggaa gatccctttc
    11281 agtcccagcg caatggtgat gttctggcgg atattggcgt gagtggcgcg tgccagttca
    11341 atcatttgca ccaggccgcg caggtggtta tgggttaatg ctgcatcggc ggtttccagc
    11401 gccacgtctg tgccgctgcc cattgcaatc ccaatggcgg cagctttcat cgctggcgcg
    11461 tcgttaatac catcaccgac catcgccagc ggcgcatgtt gattcagctt ggtcaccgct
    11521 ttgactttct cttccggcaa taggcccgct ttaaactcca gccccagctc cccggcgatt
    11581 gccgccgctg cgcgtggatt atcgccggtg aggatcactc ctttaactcc cagcgcgttc
    11641 agttcactga tggcagttgc agcgtcggcg cgcagggtat cctgcaatgc aatgataccc
    11701 agcacgtcat cattacgtac taccagcaca accgtttgcc cggcgctttc cagttcatta
    11761 atcaaaccag caaatgcatc agcgggatgt ttcccggcag cgcaaatcaa tacgcgctca
    11821 ccgttaacct gcgcttcaat gccagatccg accagcgccc gctgtgattc ggcggtggga
    11881 atggcgagtt cagcaacctg tgcttctcgc acgatggcct gcgccagtgg atgcgtcgca
    11941 ccttgctcga ccgccgctgc cagtgtcagc agttcagatt cactaatacc cgttgccgga
    12001 tgaatcgcgg taacgcgcgg tttaccgacg gtcagcgtac cggttttatc aaacgccacc
    12061 tgggtaacac gacctagttg ttccagcgcc gctccgcctt taattaacgc cccacgacgc
    12121 gctgctgccg ccagcccgga ggtaatcgcc gcgggcgtgg agataactaa cgcacacggg
    12181 cagccaatca gcagcagcgt caggccttta taaatccact cttgccagct ggcggcaaac
    12241 agcagcggtg gcaccagcgt caccagcaga gcgacggcca taatcgcggg cgtatagata
    12301 cggctgaaac ggtcgataaa ccgctcaatg ggtgcgcgac gctcttcggc ttcttcgatc
    12361 agtttcagaa tccggtcaat ggcgctggca cccggttctg acagcacttc cagcgttacc
    12421 agacggtcta cgctggtggc tccagcaggg actttatcgc ccgttgcgcg ctccaccgga
    12481 atagattcgc cggtcagggc gctttcatca aaactggcaa acggtgagag cagtttaccg
    12541 tcggcaggca aacgtccacc tgcggcgact tcaatcacat cgccagggcg caggctgtta
    12601 atcgccacct cttcccgctc accgttacgc aggcgcgtgg cggtttctgg tttcagcgcc
    12661 attaacgcgc tgaccccttg acgtgcgcgg ctggcggccc agccttccag tcgttcgcca
    12721 atcaaaaaca gcagcaacac catcgcagct tcagccgttg cgccaataaa aagcgcacca
    12781 atagcggcta cgctcattaa ggtttcaatg gcgaagtagc tgccggattt gatcaaccgt
    12841 aatgcctgac gagcaatcgg gtacagccca accagtgtgg tcgcgataaa cgccagttgc
    12901 ccgaacggat gattgaactg ctccagaccc cagctgattg ccatcatcac gattagcgta
    12961 atcagcggca gattctcttt caggcgtgat gcttgcggtt cgtcggcgga ctgttcatcg
    13021 cgcagggagt agcccgcttt ttgcaccgca gattcaacct gggcacggat gtcattgtcg
    13081 gcatcaacca ccagtttttc ggtggcgaac aacacctgta cctgattcac gcctgcaagc
    13141 tggcgcacgg cattttctac cttgcgcgca caggcggcgc agtccatgcc gctgactttc
    13201 cagctatagc gggtgccgga gacgttttca gagagagttg gcgtgctgga acatgcgccg
    13261 tcgcagcaac agtcgttggc gttctgtacc gtggttagcg gtttgaacgc agcaaattga
    13321 ggggctttct tgccgtgatt gtcaggagtc gacatggcat cctccggtta agttttttct
    13381 cattaaccga aggatacact ctggagtcga ctccagagtc aagttttatc agagatacag
    13441 cgagcggacg atcaggaagt gaccggcaaa gtagcaggcc gcagcaatcg cgttatccgc
    13501 acggaagcgt cggcgatagt gactacccag ccagacaaag ttactgataa acagcaacga
    13561 agcgccgaca aacgcagaga gcgccggagc ggtcggacgg aagaaccaca gttcacctgc
    13621 cagccacacc atcaccagcg tcatgccgat aaacgtgcag ataggccaac gatactcttc
    13681 cagccgcgtc cagataatcg ccagtaacag cgcgcccaga accagcagca ccagtggcag
    13741 aggccagaag aaagagagcg tcatctgact ggcgaaatag atggtgtaca gcaggtgcga
    13801 gaggaaaaac gcgccgatgg cgtacatcag acgttgacgt ggcaacaggg ttagcgcatc
    13861 gcccagcagt gaggcgcaca gccccgccag caccagatag ctaatggcgt cgaacatcgg
    13921 cgcttgccag gccagtaaca gcaggagaag aagggttaat ggtttaaata cccagcgttg
    13981 ccaggttggc ccacgatacg atgcatccac agatagccat gcggaaagac agacagcgat
    14041 aaacgaccaa agcatcttag ttccttgaat tcgttatttt ttcgtaagcg gaatgcctac
    14101 ggtcttgctt ccagtttagt ggcgagggtg gacggatgac aacaccataa ctggcagggt
    14161 atgcttaatt ccgcattttc cgatgaggga taaagatgag caaaccacct cttttcttta
    14221 ttgttatcat tggcttaatc atcgtcgccg catcgtttcg ttttatgcaa caacgacggg
    14281 aaaaagctga taatgatatg gcaccactcc agcaaaagct ggtgttggtg agcaacaagc
    14341 gggaaaaacc gattaacgat cgccgttcgc gccagcagga agtgactccg gcaggcacca
    14401 gtatgcgcta tgaggcaagc ttcaaaccgc aaagcggagg aatggagcag acgtttcgcc
    14461 tcgacgccca gcagtaccac gccctgacag tgggcgataa aggtacgctg agctataaag
    14521 gaacgcgctt tgtcagcttt gtaggcgaac aataaggtta ttttttaact ttaaatttct
    14581 tctgccagac cagcagttca aacacgccga aaaggaaaat ccgtaccttt tcggcggtgg
    14641 tcatctgcgg gccatctttc ggtaaagtgg atttcaatag cgccagctgc ataccatgca
    14701 tcagcacggt aaaaatcagc gccacgttaa cgaaaatatt cagcggacgt gggaagggat
    14761 gcaccaggtt gaggattaaa aatccccaaa cacagagcat taacaaacga ccaatattaa
    14821 tcagcatcac tttctccttg tgcttcgcgt tgatacagcc gataagccac ctgacccgcc
    14881 actttttccc gatgtaatga ccagtttgcc ggaacagtgg gcagaccgtt ttcgacttcg
    14941 ctttcgacat aaatcagggc ttcgtcagcc agccagccgt tatcttccag taaatttatc
    15001 gtctcttcta acaagccacg gcggaacggt ggatcgacaa acacgatatt atgcggtgta
    15061 cctttttgcg ccaggaatga catcgcgttg ctgttcacca cgcgtgcatt gcctgctttt
    15121 agtgtcgcca gattcttaat taactgctga gaaaccgcgc gatccatctc aatcaacgtt
    15181 gcccccgcag catagcgcga caacgcttcc agccccagcg cgccgcttcc ggcgaagcaa
    15241 tccagacatt gggcgtcaac aatgaccgga gccagccagt taaacaacgt ttcgcgtacg
    15301 cggtcggtgg tggggcgcag acctgggcta tcaggaaccg ggagtttacg gcctcgccac
    15361 tgcccgccaa taatgcggat ttggccgctg ccggaatgat tcggtttttt catgataaat
    15421 tgctcaatcc gccagataac acatacttgc aggcggtgat gtgaattaag ttaagtgata
    15481 gactatttca tcattttttt agctgctatg tacatagcgt taacgctgtg ccatgaagca
    15541 acagcgagga gtgtagtcgc aaatggcgaa agaaaaaaaa cgtggctttt tttcctggct
    15601 gggctttggt caaaaagagc agaccccgga aaaagagaca gaagttcaga atgaacaacc
    15661 ggttgtagaa gaaatcgttc aggcgcaaga gcctgtgaag gcttcggaac aagccgttga
    15721 agagcagccg caggcgcata ctgaagccga ggcggaaact tttgctgccg acgttgtgga
    15781 agtcactgaa caggttgctg aaagtgaaaa agcgcagcct gaagcggaag tcgttgcaca
    15841 gccggaaccg gtcgcagaag aaacgccgga gccagtggct atcgaacgtg aagagctgcc
    15901 gttgccagaa gacgtcaacg ccgaagaggt ttcgccagaa gagtggcagg ctgaagcgga
    15961 aaccgtagag attgtcgaag cggcagaaga agaagcggct aaagaagaaa tcaccgacga
    16021 agagctggaa gcacaggcgc tggctgccga agcggcagaa gaggcggtga tggtagttcc
    16081 tccggcagaa gaagagcagc cggtggaaga aattgcccag gagcaagaaa aaccgaccaa
    16141 agaaggtttc ttcgcgcgcc tgaaacgcag cctgttaaaa accaaagaaa atctcggttc
    16201 cggatttatc agcctgttcc gcggtaaaaa aatcgacgat gatctgtttg aggagctgga
    16261 agaacagctg ttgatcgccg atgtgggcgt ggaaaccaca cgtaaaatta tcaccaatct
    16321 gacggaaggc gcatcccgca agcagcttcg tgatgccgag gcgctctatg gcctgctgaa
    16381 agaagagatg ggcgagattc tggcgaaagt cgatgagccg ctgaatgttg aaggtaaaac
    16441 gccgttcgtg atcctgatgg tgggcgtcaa cggtgtgggt aaaactacga cgattggtaa
    16501 gctggcacgt cagtttgagc agcagggtaa atcggtgatg ctggcggcgg gcgatacttt
    16561 ccgtgcagca gcggttgaac agcttcaggt ctggggtcag cgcaacaata ttccggtgat
    16621 tgcccagcat accggtgcgg attccgcctc tgttatcttc gacgccattc aggcagctaa
    16681 agcgcgtaat atcgacgtcc tgattgccga taccgccgga cgcctgcaga acaaatcgca
    16741 cctgatggaa gagttgaaga aaatcgtccg cgtgatgaag aaactcgacg ttgaagcgcc
    16801 gcatgaagtt atgctgacta ttgatgccag taccgggcag aatgcggtaa gccaggccaa
    16861 actgttccac gaagccgttg gcttaaccgg catcacgcta acgaaactgg acggcacggc
    16921 gaaaggcggg gtaattttct cggtggctga ccagtttggt atccctatcc gctacattgg
    16981 tgtcggcgaa cgtattgagg atttgcgtcc gtttaaggcg gacgacttta tagaggcact
    17041 ttttgcccga gaggattaac aatgattcgc tttgaacatg tcagcaaggc ttatctcggt
    17101 gggagacagg cgctgcaggg cgttacgttc catatgcagc cgggtgagat ggcgtttctg
    17161 accggtcatt ccggcgcagg gaaaagtacc ctcctgaagc tgatctgtgg gattgagcgg
    17221 cccagcgccg ggaaaatctg gtttagcggc catgacatca cgcgtctgaa aaaccgtgaa
    17281 gttccgtttc tgcgccgcca gattggcatg attttccagg atcaccatct actgatggac
    17341 cgtactgtct acgataacgt ggcgatcccg ctgattatcg ctggtgccag cggtgacgat
    17401 attcgtcgcc gggtgtcggc ggcactggat aaagtcgggc tactggacaa agcgaagaac
    17461 ttccctattc agctttccgg cggtgaacaa cagcgtgttg gcattgcccg cgcggtggtg
    17521 aataagcccg cggtactgct ggcggacgaa ccgaccggta acctggacga cgcgttgtcg
    17581 gaagggattc tacgtctgtt tgaagagttt aaccgcgttg gggtaaccgt attgatggca
    17641 acgcacgaca tcaacctgat ttcacggcgt tcctatcgca tgctcaccct gagcgatggt
    17701 cacttgcatg gaggcgtggg ccatgaataa gcgcgatgca atcaatcata ttcggcagtt
    17761 tggcgggcgt cttgatcgct tccgtaaatc ggtcggcggc tctggcgacg gcggtcgtaa
    17821 cgccccgaaa cgcgcgaaat cctcgccaaa accggtaaat cgtaaaacca acgttttcaa
    17881 cgaacaggtg cgctatgcct tccacggcgc attgcaggat ctgaaaagca aaccgttcgc
    17941 cacgttttta acggtgatgg ttatcgccat ctccctgacg ctgcccagcg tctgttatat
    18001 ggtgtacaaa aacgttaatc aggcggcgac gcagtattat ccgtcaccgc aaatcactgt
    18061 ttacttgcaa aaaacgctgg acgatgacgc cgctgcgggc gtggtggcac agttgcaggc
    18121 cgagcaaggc gtggagaaag tgaactatct ttctcgtgaa gacgcactgg gcgagttccg
    18181 taactggtct ggttttggtg gtgcgctgga tatgctggaa gaaaacccgc ttccggcagt
    18241 ggcggtggtg atcccgaaac tcgattttca ggggacggaa tcactcaata cgctgcgtgc
    18301 tcgtatcacg cagattaacg gcattgacga agtgcggatg gatgacagct ggtttgcccg
    18361 tctggcggcg ttgaccgggc tggtcgggcg cgtttcggcg atgatcggcg tgttgatggt
    18421 ggcggcagtg ttcctcgtca tcggcaacag cgtacgtctg agcatctttg ctcgccgtga
    18481 ctccatcaac gtgcagaaac tgattggtgc gacggatggt ttcatccttc gcccgttcct
    18541 gtatggtggc gcactgctgg gattttctgg cgcattgttg tcattaattt tgtcagaaat
    18601 tctgatgctg cgattgtcat cggcggttgc ggaagtggca caggttttcg gaacgaagtt
    18661 tgatatcaat ggtttatcgt tcgatgaatg cctgctattg ctgctggtat gctcgatgat
    18721 tggctgggtg gcagcgtggc ttgccacggt acaacattta cgccacttta cgcctgaata
    18781 ataaaagcgt gttatactct ttccctgcaa tgggttccgt agcagggaaa gagatcccgt
    18841 tgtctcttcc cggtatttca tctctatgtc acattttgtg cgtaatttat tcacaagctt
    18901 gcattgaact tgtggataaa atcacggtct gataaaacag tgaatgataa cctcgttgct
    18961 cttaagctct ggcacagttg ttgctaccac tgaagcgcca gaagatatcg attgagagga
    19021 tttgaatgac tgacaaaatg caaagtttag ctttagcccc agttggcaac ctggattcct
    19081 acatccgggc agctaacgcg tggccgatgt tgtcggctga cgaggagcgg gcgctggctg
    19141 aaaagctgca ttaccatggc gatctggaag cagctaaaac gctgatcctg tctcacctgc
    19201 ggtttgttgt tcatattgct cgtaattatg cgggctatgg cctgccacag gcggatttga
    19261 ttcaggaagg taacatcggc ctgatgaaag cagtgcgccg tttcaacccg gaagtgggtg
    19321 tgcgcctggt ctccttcgcc gttcactgga tcaaagcaga gatccacgaa tacgttctgc
    19381 gtaactggcg tatcgtcaaa gttgcgacca ccaaagcgca gcgcaaactg ttcttcaacc
    19441 tgcgtaaaac caagcagcgt ctgggctggt ttaaccagga tgaagtcgaa atggtggccc
    19501 gtgaactggg cgtaaccagc aaagacgttc gtgagatgga atcacgtatg gcggcacagg
    19561 acatgacctt tgacctgtct tccgacgacg attccgacag ccagccgatg gcaccggtgc
    19621 tctatctgca ggataaatca tctaactttg ccgacggcat cgaagatgat aactgggaag
    19681 agcaggcggc aaaccgtctg accgacgcga tgcagggtct ggacgaacgc agccaggaca
    19741 tcatccgtgc gcgctggctg gacgaagaca acaagtccac gttgcaggaa ctggctgacc
    19801 gttacggtgt ttccgctgaa cgtgtgcgcc aactggaaaa gaacgcgatg aaaaaacttc
    19861 gcgccgctat tgaagcgtaa tttccgctat taagcagaga accctggatg agagtccggg
    19921 gtttttgttt tttgggcctc tacaataatc aattccccct ccggcaaaac gtcaatcccc
    19981 acgcagattg ttaataaact gtcaaaatag ctattccaat atcataaaaa tggggtatgt
    20041 tttagcagag tatgctgcta aagcacgggt agtcatgcat aaaacgaaat aaagtgctga
    20101 atcacaacac cacaacacac gtaataacca gaagaatggg gattctcagg atgaatataa
    20161 agggtaaagc gttactggca ggatgtatcg cgctggcatt cagcaatatg gctctggcag
    20221 aagatattaa agtcgcggtc gtgggcgcaa tgtccggtcc ggttgcgcag tacggtgacc
    20281 aggagtttac cggcgcagag caggcggttg cggatatcaa cgctaaaggc ggcattaaag
    20341 gcaacaaact gcaaatcgta aaatatgacg atgcctgtga cccgaaacag gcggttgcgg
    20401 tggcgaacaa agtcgttaac gacggcatta aatatgtgat tggtcacctc tgttcttcat
    20461 caacgcagcc tgcgtctgat atctacgaag acgaaggcat tttaatgatc accccagcgg
    20521 caaccgcgcc ggagctgacc gcccgtggct atcagctgat cctgcgcacc accggcctgg
    20581 actccgacca ggggccgacg gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta
    20641 ttgctatcgt tcacgacaaa cagcaatacg gcgaaggtct ggcacgagcg gtgcaggacg
    20701 gcctgaagaa aggcaatgct aacgtggtgt tctttgatgg tatcaccgcc ggggaaaaag
    20761 atttctctac gctggtggcg cgtctgaaga aagagaatat cgacttcgtt tactacggcg
    20821 gttaccaccc ggaaatgggg caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc
    20881 agtttatggg gccggaaggt gtggctaacg tttcgctgtc taacattgcg ggcgaatcag
    20941 cggaagggct gctggtgacc aagccgaaga actacgatca ggttccggca aacaaaccca
    21001 ttgttgacgc gatcaaagcg aaaaaacagg acccaagcgg cgcattcgtt tggaccacct
    21061 acgccgcgct gcaatctttg caggcgggcc tgaatcagtc tgacgatccg gctgagatcg
    21121 ccaaatacct gaaagcgaac tccgtggata ccgtaatggg accgctgacg tgggatgaga
    21181 aaggcgatct gaaaggcttt gagttcggcg tatttgactg gcacgccaac ggcatggcga
    21241 ccgatgcgaa gtaatcatta atcggcaact ttgggttgcc gccaaattgc taatatcgag
    21301 tacgttgctt catgccggat gcggcgtaaa cgccttatcc ggcctacaag atccaaagaa
    21361 atcagtaaat tgcaacacac attgtaggcc tgataagcgt agcgcatcag gcaatttctc
    21421 gttggtcatc acttttgaaa tcaacttgac gattaacgct tctcccagcc gccctgttgc
    21481 gccgtaaacc ccagcgcctg cataaacgcc gtcatcacac cgcgatcttc caagcctgca
    21541 tccgccatcc accagcatga aacgccaaga ttgttacgca aaacctcttc cagcagatat
    21601 tgccccacac cgcgacgtcg ggtgacttcc cgcacacgca gggaatccag tgctccctcg
    21661 gtgccgctta aggttacccg tacggcagcg agcaggcgct cgttaaaacg cgcggcgtag
    21721 atacggtggt tatcgtcaac ctgtaaagag gaaggggaat actccggcca gatcttttgc
    21781 aggtcaatcc ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggt
    21841 aacccgtgta aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc
    21901 tcttttttat ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa
    21961 acattagaga atgaaaaata cccagcataa tcccctgaat ggtagtgaat tattccgccc
    22021 tttgtgccgt tattttatgc tgacaaag
//
LOCUS       DAZGTR010000072        21500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGTR010000072 DAZGTR010000000
VERSION     DAZGTR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.072"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            81..2111
                     /gene="iha"
                     /locus_tag="ACHLCY_003032"
     CDS             81..2111
                     /gene="iha"
                     /locus_tag="ACHLCY_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006684920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP0370247.1"
                     /translation="MAAAEDVMIVSASGYEKKLTNAAASVSVISQEELQSSQYHDLAE
                     ALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGVRQGGSSDVTPNGFSAMNTG
                     FMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKNADKWLSSVNAGLNLQESNKW
                     GNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSLSDTAATRIPYPTESQNYNLG
                     ARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGYDRTLRYERNKISAGYDHTFT
                     FGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRELKESNLILNSLLLTPLGESH
                     LVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDEWHLTDALALTAGSRYEHHEQ
                     FGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLHKGISGVSGQGKTNLLGNPDL
                     KPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSYSINDNTNSYVNSGKARLHGV
                     EFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSYTPEHMVNAKLNWQITEEVAS
                     WLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAWTVVDAGLSWKMTDALTLNAA
                     VNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYVIPERNYWMSLNYQF"
     gene            complement(2329..2488)
                     /locus_tag="ACHLCY_003033"
                     /pseudo
     CDS             complement(2329..2488)
                     /locus_tag="ACHLCY_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2691..3011)
                     /locus_tag="ACHLCY_003034"
                     /pseudo
     CDS             complement(2691..3011)
                     /locus_tag="ACHLCY_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(3436..4011)
                     /locus_tag="ACHLCY_003035"
     CDS             complement(3436..4011)
                     /locus_tag="ACHLCY_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0370248.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(4080..4658)
                     /gene="terD"
                     /locus_tag="ACHLCY_003036"
     CDS             complement(4080..4658)
                     /gene="terD"
                     /locus_tag="ACHLCY_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP0370249.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(4707..5747)
                     /gene="terC"
                     /locus_tag="ACHLCY_003037"
     CDS             complement(4707..5747)
                     /gene="terC"
                     /locus_tag="ACHLCY_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP0370250.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(5770..6225)
                     /locus_tag="ACHLCY_003038"
     CDS             complement(5770..6225)
                     /locus_tag="ACHLCY_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0370251.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(6248..7405)
                     /locus_tag="ACHLCY_003039"
     CDS             complement(6248..7405)
                     /locus_tag="ACHLCY_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0370252.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(7405..7986)
                     /locus_tag="ACHLCY_003040"
     CDS             complement(7405..7986)
                     /locus_tag="ACHLCY_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP0370253.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            8308..9366
                     /locus_tag="ACHLCY_003041"
     CDS             8308..9366
                     /locus_tag="ACHLCY_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP0370254.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            9376..10518
                     /locus_tag="ACHLCY_003042"
     CDS             9376..10518
                     /locus_tag="ACHLCY_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP0370255.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            10511..11284
                     /locus_tag="ACHLCY_003043"
     CDS             10511..11284
                     /locus_tag="ACHLCY_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370256.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            11286..12365
                     /locus_tag="ACHLCY_003044"
     CDS             11286..12365
                     /locus_tag="ACHLCY_003044"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP0370257.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            12365..13321
                     /locus_tag="ACHLCY_003045"
     CDS             12365..13321
                     /locus_tag="ACHLCY_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP0370258.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            13332..14540
                     /locus_tag="ACHLCY_003046"
     CDS             13332..14540
                     /locus_tag="ACHLCY_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFP0370259.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            14558..15025
                     /gene="terW"
                     /locus_tag="ACHLCY_003047"
     CDS             14558..15025
                     /gene="terW"
                     /locus_tag="ACHLCY_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP0370260.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            15286..15615
                     /locus_tag="ACHLCY_003048"
     CDS             15286..15615
                     /locus_tag="ACHLCY_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0370261.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            15602..15943
                     /gene="tnpB"
                     /locus_tag="ACHLCY_003049"
     CDS             15602..15943
                     /gene="tnpB"
                     /locus_tag="ACHLCY_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0370262.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWMCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQ"
     gene            16025..16855
                     /locus_tag="ACHLCY_003050"
                     /pseudo
     CDS             16025..16855
                     /locus_tag="ACHLCY_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(16886..18499)
                     /locus_tag="ACHLCY_003051"
     CDS             complement(16886..18499)
                     /locus_tag="ACHLCY_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFP0370263.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(18530..18880)
                     /gene="tnpB"
                     /locus_tag="ACHLCY_003052"
     CDS             complement(18530..18880)
                     /gene="tnpB"
                     /locus_tag="ACHLCY_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0370264.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(18877..19302)
                     /locus_tag="ACHLCY_003053"
     CDS             complement(18877..19302)
                     /locus_tag="ACHLCY_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0370265.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            19356..20107
                     /locus_tag="ACHLCY_003054"
                     /pseudo
     CDS             19356..20107
                     /locus_tag="ACHLCY_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         5276 a         5589 c         5536 g         5099 t
ORIGIN      
        1 catgatcgga gattagtaat atgcgaataa ccactctggc ttccgtagtc attccctgtc
       61 tcggattttc agccagcagc atagctgctg cagaggatgt gatgattgtc tcggcatccg
      121 gctatgagaa aaagctgact aacgcagccg ccagtgtttc tgtgattagc caggaggaat
      181 tgcagtccag ccagtaccac gatctggcgg aggctctgag atcagtagag ggtgtggatg
      241 ttgaaagtgg tacgggtaaa accggagggc tggaaatcag catccgagga atgccagcca
      301 gttacacgct gatactgatt gatggtgttc gtcagggcgg aagcagtgac gtgactccca
      361 acggtttttc tgccatgaat accgggttca tgccccctct ggccgccatt gagcgtattg
      421 aggttatcag ggggccgatg tccacactgt atggctctga tgcgatgggc ggtgtggtga
      481 atatcattac cagaaagaat gcagacaaat ggctctcttc cgtcaatgca gggctgaatc
      541 tgcaggaaag caacaaatgg ggtaacagca gccagtttaa tttctggagc agtggtcccc
      601 ttgtggatga ttctgtcagc ctgcaggtac gcggtagcac acaacagcgt cagggttcat
      661 cggtcacatc actgagcgat acagcagcca cgcgtattcc ttatcccacg gagtcacaga
      721 attataatct tggtgcacgt cttgactgga aggcgtcgga gcaggatgtg ctctggtttg
      781 atatggatac cacccggcag cgttatgata accgggatgg gcaactgggg agtctgacgg
      841 ggggatatga ccggaccctg cgctatgagc gaaacaaaat ttcagctggc tatgatcata
      901 ctttcacctt cggaacatgg aaatcgtatc tgaactggaa cgagacagaa aataaaggtc
      961 gtgagcttgt acgcagtgta ctgaagcgcg acaaatgggg gcttgccggt cagccgcggg
     1021 agcttaagga atcgaacctt atcctgaatt cattactgct tacccctctg ggagaatctc
     1081 atctggttac ggtggggggc gagtttcaga gctcgtccat gaaagacgga gttgtccttg
     1141 ccagcacagg tgaaactttc cggcagaaaa gctggtcggt atttgctgag gatgagtggc
     1201 atctcacgga tgcacttgcg ctgactgcgg gcagccgcta tgaacatcat gagcaattcg
     1261 ggggacactt cagtccgcgt gcatatctgg tctgggatgt ggcagatgcc tggacgctga
     1321 aaggcggtgt gaccacggga tataaggcac ccagaatggg gcagctacat aaagggatta
     1381 gtggtgtgtc cgggcaggga aaaacaaatc tacttggtaa ccccgacctg aagccggaag
     1441 agagcgtcag ttatgaggct ggggtgtatt acgataaccc cgccggtctg aatgccaatg
     1501 tcacaggttt tatgactgac ttctccaaca agattgtctc ttattccata aatgataaca
     1561 ccaatagcta tgtaaacagc ggaaaggccc ggttgcacgg tgtggaattt gccggcacat
     1621 tgccgctgtg gtcagaggat gtcacgctgt cactgaatta cacctggacc cgaagtgaac
     1681 aacgtgatgg tgataacaaa ggtgcgccgc tgagttatac ccctgaacac atggtgaatg
     1741 cgaaactgaa ctggcagatc accgaagagg tggcatcatg gctgggtgcc cgttatcgcg
     1801 ggaaaacacc acgtttcacc cagaattatt cgtcactgag cgctgtacag aagaaagtgt
     1861 atgatgagaa aggagaatac ctgaaagcct ggacggtggt ggatgcaggt ctgtcgtgga
     1921 agatgacgga tgccctgacg ctgaatgctg cggtgaataa cctgctcaac aaggattaca
     1981 gtgacgtgag cctgtacagt gccggtaaga gtacgctgta tgccggtgat tacttccaga
     2041 cgggatcatc aacaacagga tatgtgatac ctgagcgaaa ttactggatg tcgctgaact
     2101 atcagttctg ataataacaa aacgctatca ctgacggtag aatacgttgc cactgcaact
     2161 cctggcggaa cagtggcaac gtattaggtt aagtgcattt ccgatccgct aatgagattt
     2221 cgttaccaac aactaatatc gtcacaggaa atgcacggat tatttttaac ttatcattta
     2281 catacttgtc cagagtgtaa gcgcaccgtg aaggacgtgg ggtaaaaatt agtttacaga
     2341 gagagtgacg ttccagggca acaactcttt catgcggttg gcaggccagg tgttgattac
     2401 actgatcacg tggcgtagcc acgtttccgg ctcgattccg ttaagttttg cagctaccga
     2461 tcaggctgta catcactgcc gcactatcgc tcgtcatctc aaagtccggt ctcgtcagca
     2521 ggaaggtatc attctctccc gccatttttc caggggcccg gtcagataag tccctttgtc
     2581 tatcgctgac tcctgactca taacccggtt agcagaatgc agggtcacca cctcgccacg
     2641 accaaatcca aataagtcaa ttgcaccttc tcaatcgcca ttttgtcagt aagcgtacag
     2701 cctgaactga tggtatcttc accatcaatg acaacggtga tcgcaatttt actgacgttc
     2761 gccggaacac gatccagtgc tatctcaatg ctggcctgct gcgaaccggt aacgagcctg
     2821 actgccccct caggagaaga caaattatta taaaagataa agtcagaatc gccactgacc
     2881 tttccctgag cattaagcat gaacaggcag gtatcgggtt cgcctttaaa gccggatttg
     2941 gcagggtact gaagattcag cctgatcgta gattgctgaa ggggtatgtt ttgtccggat
     3001 tgtaaattca tattaactct cctgattttt gattattatt aatgcgcagc gtttatatat
     3061 gttccctagg cttagttctg gacgctggat attcggtgag gcgtaaatat ggtatgacac
     3121 catttttcat aacgctgaag tttctatacc tgttgaattt gaattttcat tgaccgggta
     3181 tcttattttc cagggcccgt tccttcataa gtcgcaaaag tacacatata tccgaagggc
     3241 atgctgttga tatcagacac ggaatactgg ctttaaccag caaccacaga taaacccggg
     3301 gcctgcataa aaggttcatg ccagaattaa catagctctg ctttctgccc cccccccttt
     3361 cccatattca cccgttgata gcggatcgat acccaaaaaa aaccccggca ttgccggggt
     3421 cagactcagc gtcttttaga cattgacgcc gtgctgggaa gcaagcgctg acagaccacc
     3481 ggcaaaaccc tggccaacag ctttaaactt ccactcagta ccatggcgat acagttcacc
     3541 gaagaccatt gcggtttcgg ttgaggcgtc ttcagacaga tcgaaacggg caatttccgt
     3601 cccgttgtcg ttgttgtaaa cgcgcatgaa gctgttgctc accatgccga agttttgttt
     3661 acgcgcttct gcatcataga tggtaacggc aaataccagt tttttgatgt ctgctgagac
     3721 tttggtcaga tcgattttga cctgctcatc gtcgccgtcg ccttcaccgg tacggttgtc
     3781 gccctggtgc tctactgcgc catcagggct ggttttatta ttgaagaaaa tgaaatgggc
     3841 atctgacagt actttaccgt cttcacctac tgcgaatacg gaagcgtcca gatcaaaacc
     3901 ctgaccatcg gttacacggg catcccagcc caggccaacc atagcgacat tcatggttgg
     3961 tgcttctttg gtcagagata cgttgccgcc ttttacgaga gaaactgcca tttttagctc
     4021 ctgcaaacag ttgaatgagg ctgaattaac agccccagat atgaaaagtt acttttcgat
     4081 caggacgcgt taatgccgta ctgagcacat acagatgcca gaccaccagc ataaccctgg
     4141 cctactgcgc ggaatttcca ctcaccattg tggcgataca gctcgccgaa cagcatggca
     4201 gtctcagtgg acgcatcttc ggtcagatcg tagcgagcga cttcagtctg gttatcgtca
     4261 ttaaccagac gaataaacgc accggatacc tgaccaaagc tctggcgacg agcctgagca
     4321 tcgtggatgg tcacaacgaa gatgatcttg tcaacttcag acgggacggc gtccagttta
     4381 attttcagcg attcatcatc accatcgccc tcaccggtgc ggttatcgcc ggtgtgcgtt
     4441 acggaaccgt cggatgacgt caggttgtta tagaagatga aatctgaatc gccgcgcact
     4501 ttgccgtttg aggccagcag gaatgctgaa gcatccaggt caaagtcctg accgtctgtt
     4561 gaacgcgcat cccagccaag gcccaccagg acatttttca ttgacggagc tgctttactc
     4621 agggagacgt tcccgccttt ggaaagagaa acactcataa aataacctct tcgattagta
     4681 attgttcagg ttaacactta aggggattaa ctcccctttt cctcagattc aggtttgccc
     4741 gggaacatga cgcttgcgag aatgcccagc gccagtacac ccagtacaac atacaggctg
     4801 gttgttgccg cgatgctgta accatgatgc cagatgtgat cggtcgcatt caggccgagt
     4861 tttgccgcga tgaagaacag cagcacgata acggccttct ccagatgaac caggtactgt
     4921 ttcagtgcct caaggacaaa atacagagta cgcagaccca ggatagcaaa catcatggca
     4981 ctatagacga taagcggttc acgactgacg gcaatgattg ccggtaccga gtcaaacgcg
     5041 aacattacgt ccgagagttc aaccacagcc acacacagga acagcggggt cgcatacagc
     5101 gccgcttttt taccacggcc aatggtgaca tcgctgtttt ctggtttcgc cagttcagca
     5161 tccacttcct tctggttaag taggaaggca tgccctctga gcttcggcca gatagggaag
     5221 aagcgtttaa ccatacggta agccagatgc tgggagtaat cctcaatttc atcatcgtca
     5281 tcaccgcttt taagcatcat gaccgccgtc caggcaacga taatagcgaa gacaacttca
     5341 acgtacggcc ccagactcag caggctcgta ccgatggcga caaagatgcc cctgaagacg
     5401 atggcaccaa tgatccccca gtagagaaca cggtggcgat aacgatccgg aacggcgaac
     5461 caggagaaga ttgccatcat gacgaacagg ttatcgaccg acagcacttt ctccagcgca
     5521 taacccgtga caaacagact ggcaacctca gcaccgtggt ggatatagag gaaaccggca
     5581 aatgccatcg caaccacaac ccagaatacg gaccagagcg cagcactctt cagcgagatt
     5641 ggcttgtcat cacggtgcat aaacaggtca ataaagatgg caccgactga aagcgcaata
     5701 aaaacaatga ccgtttcagt cgggaagccg atgtgtgtgg aaaccatatt ttacccttag
     5761 ggatgtggat cagaggccaa attcagccgg gtcgaagccc aacgcgatag cgatctcgcg
     5821 ggaggccagt ttctcgtcct gatcgaagtt accgtcactt ttcgcaacgg caataccaac
     5881 acgtaaggcc agctgagcgg cctcaggctg atctttcagc gccaggatgt atttcatggt
     5941 ttcgcccttg ccaatttcaa catcgaaatc gaagcttgaa accagtttat tgaagaactc
     6001 aatcacctca ttggtatcga agaccttcag ctcttctgaa gagcgcagaa agcccatcat
     6061 tttctgcttt tcttccgaac ttacgccgtc actcgatacg gcaatacggg cacatacagc
     6121 aacggtgccc tgcatgaatt ttttgttttt gaaacggcca acctggcggg tcagttcgtc
     6181 acggcctgag ttaatggcac ctttaacttt gtcgaaaaag ctcatacctg tttcctttgt
     6241 tgctgtgtta tttagaaccg gcgctccagc gaaatcccca gccaaatgcc cggtccattt
     6301 cgtcctggcc tttgaagtac tggttaattc gctcgacttt aattgcgccg ttttcgttta
     6361 cgagtctggc aatggcgcac aatgtgcgac ggttctcacc ttcggtcagg cgggtctcaa
     6421 tgggcggttg atccggcaca tgaatggtga ctacaccatc agtcttgtcc cagctgggaa
     6481 caccttcata aataaaggcg taaatcagca cttcgcggat atgcttccat tcacgtccat
     6541 tgatatgcag ccactcgccg tctgatacat cacccgtccg gtcatcgccc ttgagctgaa
     6601 catacggttc atcgcgataa tcaccgaatg cattaccgag cgcctgaatg accgatttat
     6661 acccgtcctg aagctcaaca aaggccccca gatccaggtc gattcctttg gagccgaaca
     6721 tacctgcaaa gcctgatttg ctactgcccc ggtgccagtt caggttgatc cggatttcac
     6781 cgaagttatc ccgtttggtc aggctgattg caggcttctc ttttgtcagc gataccttgc
     6841 tcaggctgat ttttgtctca actggcggag gggttacaac cggaggagtt ggggctgcgg
     6901 gagccggttc gtcggcaata tttacgccga aatgctctgc cagcggttta agtccaccgt
     6961 tgaacccctg cgcaacgaag cggaatttcc agtcattatt acgacggtaa aattccccca
     7021 gaattaaggc tgcttcctga cggccgctca gttcgacact gccgctaacc aggccggtag
     7081 ccccttgctc cacgtcgatg gaaagattac gaagaccgga caccgtctgc ccaccgtcac
     7141 aggtcacggt gaacgcaatt ttctggacat caggtttcag ccggttgagc gcgacagtaa
     7201 aggttgagta ctggccttca ctgaccaggc tgacggtacc atcatcgtta cgtggctgac
     7261 cataaaacac catgtccgca tcaccctgca ccttcccgtc ggcatacagg cgaaacgccg
     7321 aagcatccac ctggccgccc gaggtgatcc ggacccgcaa ctcctgggct ggaacggggg
     7381 cattgccccc cggcgtcagg ttcattagcg aataaccgct gaaacgatat cggagtgcat
     7441 atcgtcaatt gtgcggccgc gacaggcatg gccaagggcg gtaaagtccc agttgccgtt
     7501 attacggcgc agcgaagaaa taacgatccc ggtatgtgag ccctgctcag tcagcttgta
     7561 gcgagccagc tctttgccgg tttgatcgac aacgcggcag aaagcgtttt cgacgtcatt
     7621 gaacgactga ccacggaagc tgtttaccgt aaacgccagg tattcaacgt ttgcaggcag
     7681 gcgggagagg ttgaccttaa tcacttcatc gtcaccgtca ccctcaccgg tcaggttatc
     7741 gccactgtgc acaacagcgc cgcaggtgga tttcagtttg cggaaccaga tggtgtcaat
     7801 tgttttacct gtgctgtcca ttaatacaca gccagcatcg agatcgattg agtcgttgcc
     7861 tccaaacaat cctccaagaa gacctttttt cttaaccgga tcccaaccga gaccgaaatg
     7921 aagctggctt aatgcagatg attctttgct gagtgatacc gtctggttct taactaatga
     7981 aaccatgttt tattccttaa aatggtaatg accgagacaa tcaaaggtat atttagtaac
     8041 tgacgaagaa aatcatatca tgatgaagtt ctctgacaag ggaaattttt gtggttttct
     8101 gtaaaaacac aaaaagaaat ccttttcagg gtgttggttt ttctattttt gatttaaata
     8161 tcaatgtgtt ataaggaatc caaaaaacac tgacgcactc cataaccaca ggagttatta
     8221 atcatattat gattgacatg tagcttagac actcatagac tcacttctca atcataaatt
     8281 tgcatggcgt tccggataat taaaaaaatg aacaagaaaa tttggtttat ggaaggttta
     8341 tcctcccagc gagatattat tcagggggta aaatcatttg cacaaaaaaa taattttgcc
     8401 attaccgttt ttgcctccca ccgtaacgaa agaaatgaaa tcctttccgt tgccgattat
     8461 tctttgactg aacctgaaga tcctcaaaaa cgtcttcagt ttatccagga aaccattcag
     8521 acctacggca tccaccatat tcatactggc cgtaacagcc agtggtttga agaacaccgt
     8581 tcagccattg aaccgaccgg tgccaccctt actaccggtg caacgggcgt cgactggtta
     8641 actctggctg acgaaaaagt tacttttgct cagtttatgg agcaaaaggg tctcccggtc
     8701 gtaccatcct ggcgggtgaa tacgctggca gaattaaaga cacacctcgc ggccccgccg
     8761 ttcactgaca gcccggtatg cgtgaagccg gtgacgggta tctatggcat gggattctgg
     8821 cgctttgatg acagtgcttc gcctatggcc gtctttaatc atcccgaaca tcgtctggtc
     8881 agtccgcaac agtatattgc agcagcatca gctgctgagt cgtttaaacc ccttgttttg
     8941 atgccgtacc tgccaggccc ggaattttcc gtcgatatcc tcgcggataa gggcgaaata
     9001 ctcgcagccg tgggacgccg taaggaaggg gctatccagt atctggtaaa cgaaggaagc
     9061 gcctgggaac tggcgtgtga ctgcgcccgt gttatgaagg ccgacgggct ggtgaatgtt
     9121 cagacgcgaa acgatgtgaa tggcaacccg gtgctgcttg aaaccaacat gcgtccgtca
     9181 gggggggtgg gttataccct tcacagcggt gtgaaccttc ctgggttatt tgctgccttt
     9241 aagctcggtc tgatgtctga agatatggta cgccagagcg ctaaaaacac cttttctccg
     9301 gttgcggtga gatccattac ggatgtaatt gcatacccgg aatcactctc taaccttctg
     9361 aattaaggcg tatttatgtc tgatatcctc gaagatatga tttatcgccg taccctctca
     9421 tgcggtacga ttcaggtaac ccgtgaccag ggtgaagttt cgctcgatga cctgtttgac
     9481 atcgctgaaa gacgtaaccc gaaacgcgcc tttctgtttg tcagcaaagt actcggcagg
     9541 cacattcccg tgccaccctc agtcatgcgg caggcctaca gacagcttgc cagccagttc
     9601 ccctcgacac tgacaggacc cgtactgttt atcggtatgg ctgaaaccgc cgttgggctt
     9661 ggggccggtg tatttgatga agtgcgccac cagcatcgtg aatctgtcta tctgacctct
     9721 acccgtcacc cggttgatgg cacgttgctt tgcgagttca aggaagaaca cagccacgcc
     9781 accgatcatc tgatctatct gccagatgat gaagagaaaa gacgtcgtgt taccaacgca
     9841 cgaacgctgg ttttgattga tgacgaggca accaccggta atacctttat taacctgctt
     9901 tctgccctgc gtaataccgg caagcttcaa catattgaac aggttatcgc cgtaacgctg
     9961 accgactgga gtggcaatgc cctgtcggag cgcaccccct tgcccgtcac ttcggtttcg
    10021 cttgtaagcg gtcagtgggg atggacccca ttacctgatg cacctgtccc ggacatgccg
    10081 aaagtcaacg taacttcacg aggagaatgg gacatccagg ggaaacagtc atggggccga
    10141 ctggggatgc tcgcacctgc ggccgatctc ggccatgagg tctccgtcca caagggggaa
    10201 cgtattctgg ttctcgggac cggggaattc gtctgggagc cgttcctgct tgctgaacgg
    10261 ctcgaagctg ccggagcaca ggcattttat ggatcgacca cccgctcccc tgtcgccgtt
    10321 ggttatgcca ttgagtccgc catttccttt acggataact acgggctggg catccccaat
    10381 tttgtctata acgtcgccca ccagcagttt gaccgcattc ttgtgtgtac tgagacaccc
    10441 gcagaaagta ttgacacgca gcttcttaaa gcgctggctg aggttgcgcc cgtcgtggag
    10501 attgttacct atgaataaac ccgttgttct gagcgacctt gacgacacgc tctttcagac
    10561 ccgtcgtaaa atggtggacg agctggccct ggagccattc cgtaccggtg ccgttgatcg
    10621 caccctgaat ccacgaagct ttatgacgga agaacagtcc atgttggtgg actggctcct
    10681 ggagcaggct gaactcatac ccgttaccgc acggggaact gaagaaatca gccgcgtacg
    10741 gatccctttc cactcctggg caatcacaac gcacggggcc gtcattctca cgcctgaagg
    10801 caaacccgac gaggagtgga aggcccatat gctcggccag ctggctccct accaggaaaa
    10861 gctgacatcg atgcagcgtc tgatcactga aatgatggac gcaaaaggaa tcaatgcctg
    10921 ggcaaggctg aacttcgaat acggtgaaac ggcggtttat atggtgatga aacaccgcga
    10981 cagcacccgc cttgacgagc tcaatgccat tgcagatgag atagaaacgg tgtttccgac
    11041 cgagggcttc tacatccacc gcaacagtaa taacgtggcc tggcttccca ccccggttga
    11101 gaaagggctg gcagtcagct ggctgcttga aaaacttcgg gctgaacgag gagttttccc
    11161 cgtaattggt ttgggcgaca gcctgagcga tcatcgtttt atgaaactgt gcagctggtt
    11221 tggcatcccg cgtcagagtc agtttgcaga tgccatttca cagcgaattt ttggagaaaa
    11281 ttaaaatgca gccacatgaa acatttaccg gctcatacca gcccggtgac gtggaatttc
    11341 tgctaaagcc ggttgtcatt gagatgacgc cggttgagaa aaaagaagaa ctgattcagt
    11401 ccgggaagaa acattattcg gacatgctca gccaggagcc agcgcctaca caatggcatc
    11461 ttgatttgtt tcaccgggcg ctggatcggg gcgcagagag gcttgcaaaa gaagtcacac
    11521 agctcgctat tgctctggcc gaacgcttcg gtgatgagcc cattgtactg gccagtcttg
    11581 tcagggctgg cgttccgctc ggcgttatgc tgcaccaggc cctgcgtgac atggggaaaa
    11641 cctcatggca ttacggtatc agcattatcc gggatcgtgg aattgacggt gcagccctcg
    11701 acgtcattga agagcggcat ggtaccagcg gtattgtctt tgttgacgga tggacaggta
    11761 aaggcgcaat taccggagag cttgtacgcg cactgaaaga tcgcccgggc tatcctgagc
    11821 agccgcgact ggttgtcctg gccgatccct gtggctgctc ctggcttgca gccagtgatg
    11881 atgactggct cattcctttt ggcatcatgg gggcgccggt atcaggcctg atctcccggt
    11941 cagtatggtc ctctgaagga ttacacgggt gcatggtttg cgagcatctc agtgaattcg
    12001 aatgcagccg gatgcttgcc gataccgtcg ctcatttccg taagaagtta acaccgtcat
    12061 ccctcgctcc cctgagctgg aatacggaat cagcccgggt cttatggcag acaagtcgcg
    12121 acgttatcgc gttcctggcc gatgaattta aagtggacag cgtcaatcgt attaaacccg
    12181 gtattgctga agcaacccgg gctgtattac gtcgggtacc ggaccatgta tttgtgcgtt
    12241 ctattgacga cccggacgtt gccttgcttg tagggcttgc tcgtgaaaag ggaatagttg
    12301 ttacagaaat ggggggaacc ctcggccagt atcgggctgt aaccattatc aagaaggtac
    12361 tctgatgaaa aatcgtcttt ctccctggaa tctgggagcc acgctataca tgcctgcaac
    12421 acgggaagat attgctgatg ccgtcctgca cgggaaaatc ccgggcttgc gctctctggt
    12481 gatttgcctg gaggatgctg tcagtgaagc agacatcccc gtcgccctca aaaatctgga
    12541 gcacctgctg cacgagctca gtaacagcat gagtagcctg ggtaaaaatg actggcctct
    12601 ggtgttcatt cgcccccggc atgccgaaat gggcagatgg ttaaaagcgc attatgatct
    12661 ttccgctgtc gacgggtttg tgctgcctaa atttaccctc agttcgttag ctgaatggtg
    12721 ggatatcatg gtcgggactc acctgtgcat gatgccaacg ctggaaacag aagatgtctt
    12781 tgacgtggtt cagatgcgcg agctggctac ccgtctggag gaacatccct gccacgaccg
    12841 cattattgcg cttcgaatcg gcggcaacga tcttatgaac gttgtgtcgc ttcgccgtcc
    12901 ccgggacctg acgctgtatg acagcccgat gggctacgtc atcaaaatgc tcgtttcggt
    12961 cttcggcccg cgtgattttg ccctgaccgc gcctgtatgt gagcatattg acgatcatgc
    13021 cgttatggcc agagagctgg ctcttgatat ggcacacggg cttgttggga aaacggccat
    13081 tcacccgggt cagatagagg tcattcaaaa cgcgctgatg gtcactcagg gtgagcattc
    13141 tgacgctctg cggatcctga actctaccca ggccgtgttt aagtcgcagg gagcaatgtg
    13201 tgaacccgcc acacatcgcc gctgggcggc tggcattctg gacagggctc gtttttatgg
    13261 gttacagaac gagcaaagcg ctgatggaat cagattactt accgtgaccc agcatcatta
    13321 aggaaatcat aatggggttc aggttcagaa agtcgatcaa cattattcct ggcgttcgcc
    13381 tcaacctgag taacggtgca ccgagcctga gtgtcgggcc gagaggtgct tccgtttctt
    13441 ttggtagccg ggggacctat gccaatctgg gcttgcccgg taccgggctg agttaccgta
    13501 cccggcttga ccgggccgcg cgttccagag gtgaaaaccg gacggcaacc gacccggggc
    13561 tcagacaggc gcttgagcag aaagccgctg aactcatgtc agcggtaacc gcaatccgta
    13621 atattcacga gctgacgccg gatccaaaaa caggcatcag ctgggcagag ctggaagcag
    13681 tatacctgca taacagaacg tcgccttttc aggttccggc accggtgcgt ccagaaaagc
    13741 cagactacct tgtattgccg gaaaagcctg ccgagagcga aggcattagt tttttgggta
    13801 aatggtttga atcggaatca gctaaagctg agcgccacgc cgaaaatctt cgccggtggc
    13861 agcaggagct gattgatgtg gagcgtgaga atacccttcg acagcaccgg taccagcaac
    13921 aacggacggc ctgggccgaa cagtatgcaa actggaagtt tgaagctgaa gaacatgaaa
    13981 aacggctcgc cacggctcag gcagatgccc ggcagcagtt ccggacagac gccgcgtttt
    14041 tcgaatcata cctggcgggt gtgctggcag aaactgaatg gccgcgtgaa acgcttgttg
    14101 catttgaagt aaagccggag ctatcagcag tcctgctgga cgttgattta gctgagattg
    14161 aagatttccc tgataagatt tacggcgtta atgcccgggg aacggagctg acggaaaaag
    14221 ccatgacgca aaaagccgta cgcgaaaact atgcccacca cgtccatggc tgcttgttcc
    14281 gcctggtcgg tatcgtttta catacgctac ctttcgacaa cgtgattgtg tcaggcttta
    14341 cgcaacgggt cagtaagcgg accggctatc tggaggatga gtatatcctg tcctgcaaat
    14401 gcactcgcag ccagatgtcg tcagtaaatt ttgcaggcat aaaacacatt gatccggttg
    14461 aagcgttagg cgatcaaccg gttattcgaa agatgagcag taccttcatt tttcagccta
    14521 ttgaaccact aaccctttaa gcaggctaaa tcttacgatg caattaaata ccagacaggc
    14581 ccggattttt aagcttgcca acttactggg caccggcaag cccgtttccg ctgccgacat
    14641 tataaccagt ctggaatgct ctgagccgac attaacccgg gccctcaaag agctacgcga
    14701 gtcatattct gcggagatca aatacagcaa ggcaggtcat tcctatcatc tcgttaatcc
    14761 gggccagttg gataaaaaga ccctccgccg gatgaatgaa gcgctcgcac agaatgctga
    14821 actgaaaacc ggtgagtcca cagggaaggt cgtactcgat aaggataaaa aaacagccgt
    14881 gtcattatcg ctacgcatgc ggattctaag aaaaattgac cgtctggctg cgctgagtga
    14941 ctcgacccga agtgaagcgg tagagaagct agctctgcac tccgttgatg aactgataaa
    15001 agaatacagc gctaaaaagt cctgacgcta aatctcagac cggggtgaaa actccggttt
    15061 tgtcatattt cacctctgta ttggcgtgat gcaaaatggc atctgaatcg atcatggttt
    15121 ggcatccatt tccgatcact gtttggcatc acaatcgatc acgatttggc atgcttccgc
    15181 tcattgattg gcatcctggt gtcgttacgg ggaagtcatc ttcacaggga tcttcagtca
    15241 gttagcatta ccgacatcca ttcaccaact taccggagat aatcaatgtc aaaaccccga
    15301 tggacgcttg atcagaaaaa acatcacgtt gccgcatggc gcgccagcgg ccttacccgt
    15361 gaacagtact gtgaacttta cgacattccg tttaaatccc tgcgccagtg gccgcaggat
    15421 gtcgcaaaag ccgaaaaacg agccagagca ccagaaatta ttcctgtttc agtatctggc
    15481 tcttccggaa tgactgatgg cagaccattg tcggatgagc ctgtcactct cttcctgccc
    15541 ggtggtatcc ggatgtgctg ccagccttct cagttaaccg atgttttccg ggctcttcgc
    15601 catgctgacg cctgataacg tattcctggt cgtaaaaccc gtcgatatgc gccggggtat
    15661 cgacacactg acacagtacg tccagaacga acttaacgct gcatggcatg acggtgctgc
    15721 ttttgtcttc accaataaag tccgctcccg cataaaagtt ctccggtggg acaaacatgg
    15781 cgtatggatg tgtacccgac gactgcaccg gggaagcttc cgctggcccc ggaaaggtga
    15841 tgccacatgg catctcacac aggatgagtt tcactggctg gtctttgggg ttgactggca
    15901 acaggtgaaa gggcatgacc tggcaaaatg ggtataccag taagaacccg aagatgctca
    15961 gagcaacgca ataactcaat gaaatataat gaatatccat gcgtgatgtc atataatcca
    16021 ccgcatggat atctcgcttc tcagtaccac cagtgaccct gaacagcttc gcgctctggc
    16081 aattgccatg gtgcaaaaag tcatggctga gaatgcagaa cttcagaacc gtatccgcat
    16141 cctggaagag cagatgaagc tcgcacgcca gcagcgtttc ggtaaaaaat gcgaatcgct
    16201 ggccgggatg cagcgttccc tgttcgaaga ggatgttgac gcggatatcg cagaaatctc
    16261 ggcgcatctg gacaaactgc ttcctcagac cggggacgaa gaaaaaacaa caacacgtcc
    16321 tgttcgtaag cccttgcctt cacctcttcc ccgtgctgaa aaggttatcc cgccagctga
    16381 ggaacgttgt cctgactgtg atgcccccct gcacttcatt cgcgatgaag tcagcgagaa
    16441 gctggaatat attccggcac aggtcgtggt caaccgctat atccgcccgc agtacagctg
    16501 tccctgctgt gagaaagtgt tcagcggaaa aatgccggca catatccttc cgaaaagtgc
    16561 tgttgagcca tcggtcatcg cacaggttgt catcagcaaa tataccgacc atctgcccct
    16621 gtatcgccag cagcacattt tctcccgaat gggggtggag ctccccgtca gtacaatggc
    16681 ggatatggtc ggtgttgctg gcgctgcact ggccccgctg gcaaaattgt tacgccacga
    16741 gcttctgaca cgggatgtta ttcatgcaga tgaaacgtcc cagaggctcc ttgatacccg
    16801 gaaaggcggt aaatcctgct ccggctggct gtgggcttac gtcagcggag aaagagtaag
    16861 cgtcaacgga gcaccgtatt gacgcttatt tattggtgag tactacgttc catggcagga
    16921 gttcatcaac tcggttggaa ggccattctg gcagtacgct caggatatgg cgcagatacg
    16981 cttccggatc gataccgttc agacggcagg tgccgatcag cccgtacaac agtgctccac
    17041 gctcgccgcc gtgatcgctg ccaaagaaca taaagttttt ctttccgaga cagactgcac
    17101 gaagcgctct ttccgcagca ttattatccg cctccgccag accgtcatca ctgtaataac
    17161 agagggcatc ccactgattc agtacatagc tgcacgcttc gcccagtctg gattttttcg
    17221 acagcgtacc attcttctcc accatccatt catgcagtga cgtcagtaac actttgcttc
    17281 gctgctgcct gacggcaaga cgctctgact ccggtaatcc ccgtatttca tcctcgatgg
    17341 cgtacagttc actgattcgc ttcagggctt cttctgccgt cgcacttttg ctgctgatgt
    17401 atacatcgtg gatttttcgc cgggcatggg cccagcacgc aacttctgtc agtgcaccac
    17461 cttcacgttc tgcactgaac agccgatcgt aaccgctgaa cgcatccgcc tgcaggatac
    17521 cccggaaggg acgaaggtgt tgtaccggat gttttccctg cctgtctggt gagtaggcga
    17581 accagaccgc cggtggctct gatgagcccg cattccggtc atcccggaca tacgtccaga
    17641 tgcgtcctgt ttttgccttt tttctgcccg gtgccagcac ttttactggt gtgtcgtcag
    17701 tgtgaacctt gcgggtgttc atcacgtaac ggtacagggt atcattcagc agagtcatta
    17761 actgacaaca tgcgtcaacc cagttggaga gtaatgcccg gctcagttcg atgccctggc
    17821 gggcaaagat ttcactctga cgatacagtg gcaggtgttc gcagtatttt cccgttaaca
    17881 cgcgggcaag taatccggag cccgcgatgc cgcgctctat cgggcgggac ggcgctggcg
    17941 cttcaactat acagtcacat tttgtacagg ctttttttac ccgaacagtg cggatcactt
    18001 tcagggcgct actcaccagt tccagctgct cagcactgac ttcccccaga taatccagct
    18061 cactgccaca ctccgggcaa caactttctt caggctccag acggtgtatt tcacggggaa
    18121 gatgtgccgg taacggacga cgatgacgtg actgtcgcaa ctggcgggga acctgcgggt
    18181 catcctcacg cccactgtaa cgatcgcttt cctgttcgcg ttgtttcagt tgggcctcag
    18241 cctgttcaac ctcacgctgc agtttttcag aacgggtacc gaacaacatc cggcgcagtt
    18301 tttctatctg ggccctcaga tgttctattt cccgctcctc ctcttcgatc ttttcctcgg
    18361 cacgtgccag tgcagagcgc aggaaggcct ccgtctcttc aaccagactc agttgctgat
    18421 ctttctgacg gagggcttca gcctgctcag agagcagcct ttccagctca gtgatacgaa
    18481 tgaggtattt ccgactcatg accgttttta taatccggcc atgacatttt tacaacattg
    18541 tcagtgcatt aaggcgggat gttttgggct gacgccagtc cagtttatcg aggagcattg
    18601 ccagctgcga gcgggtaatg gataccttac cgtcacgcac cgcaggccag ataaactggc
    18661 cttcctccag acgtttggtg aacaggcaca gaccatcagc atcagcccac agaattttaa
    18721 tcgtgtcacc ccgtcggccg cgaaagataa acaggtgacc ggagaagggg ttctcatcca
    18781 gcacatgttg tacctgttct cccagaccgt tgaaggattt acgcatatca gtaacgccgg
    18841 caaccagcca gattcgagtg cctgatggga gcgagatcat cgtcccctcc cggtcagttc
    18901 acggatcaac accgtgagca gctctggtga aggattttcc agcgtcatgt taccgtggcg
    18961 gaactcaact ttacaggaac tggcactgac tgtgctttgt gaaggagtgg ataaaagcgg
    19021 agtaagagcc gccataggct ctttctgctc atcaggcgtt atctcaacag gtaataattc
    19081 aacgccagcg ccagaagagg ttgttaccgg aagacgccgc gatatacgcc tttcgttctg
    19141 ccagagcctg agccatttga acaggaggtt atcattgata tcgtgttccc gggcaatacg
    19201 ggcaacagag gctcctggtt gtgaagccag tttaaccatt tgaagtttaa actcatttga
    19261 aaatgatctg cggggttctg aggataatat tttctgttcc ataacaggtg tccactagtt
    19321 gaaaaggtgg gcacctacgt taccaatact ggcttaatgg ctacatacgg cggtcagttt
    19381 acgcttacga gaaagaagcg gtccgccggt cgtctgtttt gactcgcaga ccggacgggc
    19441 cctcagatat cctgaggcct ggctgcaggg ctggtgtggc ggtacgctgg tgagtgacgg
    19501 atacagcgtg tacaaatctc tggcggataa tgaccccggt attacatctg cctgttgctg
    19561 gagccacgca aggcgcggtt tcgcaaatct gtataaggcc agcagggagc cccgtgcagc
    19621 catggcgctg agaaaaatcg cgggtcttta tcgcattgag aagttcatcc gtgaacgtcc
    19681 tgtggaaaaa atacggcaat ggcgacaacg gtattcccgg ccgatagtga atgacctgtt
    19741 tgcctggcct gaagagcagg agccgtgctg tccgcccgac ggaccactaa acaaagcgat
    19801 taattatatc ctgaaccggc gcgatgaact gagctgcttc ctgagtgacg gagccgtgcc
    19861 cctggacaac aatatctgcg agcgtgctat ccgcccggtg gtcatggggc ggaaagcatg
    19921 gttattcgcc ggttcactga tggccgggaa ccgcgcggca cagataatga gccttctgga
    19981 aacggcaaaa cgtaatggtc tggagccgca tgcctggctg acagacgttc tgacgcgtct
    20041 accggagtgg ccggaagacc gactggaaga gttgctgccc cttgagggat ttaccttctc
    20101 cgggtgagtg atacctgcca tcaggtgttc gtgcagcggg ccataacccg cagtcgggag
    20161 ttgaacgcct gacgacagga aatgagccag agcagcgacc acgcgggctg acagtgagtc
    20221 agaacctgaa tcagcccgga gccattcccg gggctgtact tcatcacctg ccatcagtac
    20281 ccgtgtcagt acttcgcgac ctgacgcaat tacccgactc agttcttcag aacccgcctc
    20341 cgataccagc ctggcggtcg cccctgtgca acaaaataaa tcaaccatcg tcagacggtg
    20401 atcaatcgta tgcgctgcca gtggctcgcc tgaaaaatgc gtcaatatga gttcgccgga
    20461 acaatacctc agttcttcag aacccgcctc cgataccagc ctggcggtcg ccccggcgca
    20521 acagaataaa tcaaccatcg tcagactgcg atcattcgca tgcatcgctg atgatgcgcc
    20581 cgaaaaacgc gtcaatatga gttcgccgga atattactga cgacacggtt tgtagttacg
    20641 ttccttcggt gagtgagtac ctgtgcgtca gggtaacgca atcagttcag gtttagcggg
    20701 gtgtcaagtc ggtttggcta accgaccgca gaattaactg tacctttaaa ggtcgccccc
    20761 gatatatctg cctgtcagac atatcgccga ttcggtatct cactacataa aactccttaa
    20821 aaaacaatgc gattctgttg gtacgaagct ttcagtaagt cagtccggag aagcgccagt
    20881 acgatacaga atgaggttga aaaaaacgtt cacgtctggt cacttcttca aatgttaaaa
    20941 aacatctcgt aaagagccag tttatgcggg ttcgttgagt agacgtgcga gcgtcacttt
    21001 gtcagcttta acgctgacga aagtgacgcc cgcacagctt caaactccac atgcacacgc
    21061 aagcgaccaa atttcaaatg gtcttatgcc gtgcgcacag tcataagaag cgcatgcgga
    21121 aagtcagaaa atatcttaag acgcagattt taaaggcgtt tttatgccag tctgccggga
    21181 atacacagta gtccggttgt ccggaagcac cacggtaatc cgaaggaaaa atcaacgtac
    21241 ctcaatcacc gtagtgatat ccgttcccag aggctaaacg gatgtaccac cataaaggca
    21301 tcctctttca ggctacgagg atattgcaat cacccgaagg cgtttctctc agattgctga
    21361 aacattggct ggcagtcacc cgaaggcgtc cctctcagac tgctggaaca ttggctggtc
    21421 gcaaaagcga cagttaatgc aaatacaggt taacaaaaag ggaaatctgc cgtcaaccca
    21481 accatatgaa catgttgatt
//
LOCUS       DAZGTR010000073        21458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGTR010000073 DAZGTR010000000
VERSION     DAZGTR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.073"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..327
                     /locus_tag="ACHLCY_003055"
     CDS             <1..327
                     /locus_tag="ACHLCY_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0370266.1"
                     /translation="KITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKW
                     SLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQ
                     PVSLVG"
     gene            complement(370..1521)
                     /gene="ydiO"
                     /locus_tag="ACHLCY_003056"
     CDS             complement(370..1521)
                     /gene="ydiO"
                     /locus_tag="ACHLCY_003056"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP0370267.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKVYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1535..3129)
                     /gene="ydiF"
                     /locus_tag="ACHLCY_003057"
                     /pseudo
     CDS             complement(1535..3129)
                     /gene="ydiF"
                     /locus_tag="ACHLCY_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
     gene            complement(3273..4031)
                     /gene="aroD"
                     /locus_tag="ACHLCY_003058"
     CDS             complement(3273..4031)
                     /gene="aroD"
                     /locus_tag="ACHLCY_003058"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP0370268.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHETPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4062..4928)
                     /gene="ydiB"
                     /locus_tag="ACHLCY_003059"
     CDS             complement(4062..4928)
                     /gene="ydiB"
                     /locus_tag="ACHLCY_003059"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP0370269.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4940..6205)
                     /gene="ydiN"
                     /locus_tag="ACHLCY_003060"
     CDS             complement(4940..6205)
                     /gene="ydiN"
                     /locus_tag="ACHLCY_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0370270.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6432..7646)
                     /gene="ydiM"
                     /locus_tag="ACHLCY_003061"
     CDS             complement(6432..7646)
                     /gene="ydiM"
                     /locus_tag="ACHLCY_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0370271.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYEQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7746..8102)
                     /gene="ydiL"
                     /locus_tag="ACHLCY_003062"
     CDS             complement(7746..8102)
                     /gene="ydiL"
                     /locus_tag="ACHLCY_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP0370272.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8238..8345)
                     /gene="rprA"
                     /locus_tag="ACHLCY_003063"
     ncRNA           complement(8238..8345)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHLCY_003063"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8531..9643)
                     /gene="ydiK"
                     /locus_tag="ACHLCY_003064"
     CDS             complement(8531..9643)
                     /gene="ydiK"
                     /locus_tag="ACHLCY_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP0370273.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVVQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10032..13088
                     /gene="ydiJ"
                     /locus_tag="ACHLCY_003065"
     CDS             10032..13088
                     /gene="ydiJ"
                     /locus_tag="ACHLCY_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP0370274.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13085..13495
                     /gene="menI"
                     /locus_tag="ACHLCY_003066"
     CDS             13085..13495
                     /gene="menI"
                     /locus_tag="ACHLCY_003066"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP0370275.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13595..13783
                     /gene="ydiH"
                     /locus_tag="ACHLCY_003067"
     CDS             13595..13783
                     /gene="ydiH"
                     /locus_tag="ACHLCY_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP0370276.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14331..14699
                     /gene="sufA"
                     /locus_tag="ACHLCY_003068"
     CDS             14331..14699
                     /gene="sufA"
                     /locus_tag="ACHLCY_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP0370277.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14708..16195
                     /gene="sufB"
                     /locus_tag="ACHLCY_003069"
     CDS             14708..16195
                     /gene="sufB"
                     /locus_tag="ACHLCY_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP0370278.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16205..16951
                     /gene="sufC"
                     /locus_tag="ACHLCY_003070"
     CDS             16205..16951
                     /gene="sufC"
                     /locus_tag="ACHLCY_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP0370279.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16926..18197
                     /gene="sufD"
                     /locus_tag="ACHLCY_003071"
     CDS             16926..18197
                     /gene="sufD"
                     /locus_tag="ACHLCY_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP0370280.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18194..19414
                     /gene="sufS"
                     /locus_tag="ACHLCY_003072"
     CDS             18194..19414
                     /gene="sufS"
                     /locus_tag="ACHLCY_003072"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP0370281.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19427..19843
                     /gene="sufE"
                     /locus_tag="ACHLCY_003073"
     CDS             19427..19843
                     /gene="sufE"
                     /locus_tag="ACHLCY_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP0370282.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            19992..20996
                     /gene="ldtE"
                     /locus_tag="ACHLCY_003074"
     CDS             19992..20996
                     /gene="ldtE"
                     /locus_tag="ACHLCY_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP0370283.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21060..21296)
                     /gene="lpp"
                     /locus_tag="ACHLCY_003075"
     CDS             complement(21060..21296)
                     /gene="lpp"
                     /locus_tag="ACHLCY_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP0370284.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5581 a         5224 c         5426 g         5227 t
ORIGIN      
        1 aaaatcactt tagaacagtt atcgaaaaaa tttcgtgcca gcgtcagtta tatttgccat
       61 gaatttacca aagagtatcg tatttcccct attaactatg ttattcaacg gcgtatgacg
      121 gaagcaaaat ggtcactcac taatactgaa ttatcacagg cagagatctc ctggcgtgtg
      181 ggttatgaaa atgtcgatca ctttgccaaa ctgtttttgc gccatgtcgg ttgttcgccc
      241 agcgattacc gtagacaatt taaaaactgt tttgcggaac aagaaatcct atctgaattt
      301 cctcaaccgg taagtcttgt tggataaatc tggcggcgca gtttgttaag aagctgcgcc
      361 gcctgcagat tatttgttct gatagtcttt caggatctgc cgacctgcta cgtaaatcat
      421 aatttcgtct gtaccgccgc cgatacgttc acaacggaca tcacgccaga agcgggagac
      481 gcgcgcttca tcggtatagc ccagaccgcc catgatttga atcgcatcat caatgacttc
      541 cattgcggta cgtgcgcaat acacttttgc cagcgccgcg ctggtgcgca gtgactgatg
      601 ctgatcggct tgccatgcca ctttcagcac catgttgcgc atgttgtcga tcttaatcgc
      661 catcagcgcc agtttttcct ggatcatctg gttatgacca atgggcttac caaaagcgat
      721 acgttggttg gcatagcggg cggcatcttc aaaggcgcat tcggcaaagc cggtgctgcg
      781 cgcggcgttg atcaggcgct ccatctcaaa gttgtacatc acattgagga aacccattcc
      841 ttcttcgccc accatgtcgc tctcttcaac ttccacattg tcgagataga cttcgcaggt
      901 gctgagcata tgccagccga ttttatgcag cgggttaatc ttaatgccgg gcttactgga
      961 gtcgacccac cacagggtga aggctttttt gggatctttc ggttgcggat cgcgcgccag
     1021 caccagcata tacgggtact ctttcgcacc ggtaataaag gttttttgtc cgttgatgta
     1081 gaccttgccg tttttacgcg tgtaagtggt agtggcactg ttgttatctg agccagcacc
     1141 tggttccgtc aacgccaggg cataggcggg atcacccgtt tccagggtgc tctctgccgt
     1201 tttacgtagc tgctctgcag aaccgaaacg gcgcatactg tgaatacatt gaccgttggt
     1261 aattaaaaat gccggagcac cgcattttga cacttccatc agcgccagca tttgggtgac
     1321 gtaatccgca gggataccac caaattcttc cggcacgcca agcatggaaa taccgttatc
     1381 cgccagcgcc cgcataaact cacgcggata tgtcccgttt tgatcgcagg tgcggaaata
     1441 ctcttccgga aagttagtcg taatcagttc gcgaatactg gccagcagca gttcttgttc
     1501 ttcagttaaa gaaaaatcca tcattatact cctgttaatg agccgcttca ggcaggacaa
     1561 aacccatcgc cgcatcgata aataatcttt cgtccatcag tttgagttct ggcgaaatca
     1621 ctggggtgaa gtccattttg tcgagaatat ctttttgtaa atcgacgcca ggggcgattt
     1681 cgattaaatg caggccgtct tctttcagcg tgaatacagc acgttcggtg atgtaacgaa
     1741 catccagtcc ccgttcgagc gcgatttttc cgctaaaagt aatttccggt agttcccgaa
     1801 taaatttatt tacccgtcct tcctggacga tatgcaattt gccatcggca atttctgttt
     1861 ttaaactgcc cgcagttaac gtgccgcaga aaataatttt cttcgaggtg gcactgatat
     1921 caataaatcc accggtaccc atgattttgc cattgaattt atgcacgccg acgttaccgt
     1981 gctggtcgac ttcagcaaaa ctcaaataac aaacatccag accaccaccg tgataaaaat
     2041 caaactggga cgtcatatcc agaatggcgc gggtattcac gttcgcgcca aaggcgatac
     2101 cctgtgaagt aattccgcca atcggacctg tttctaccgt cagaataaag tcatcagcac
     2161 aaccttcttc ccgggcgacc aggccaatac cgtcagcaat accgacgccg acattcccca
     2221 ccgcgccttt acgcatttcg aataatgcac gccgcgcaac taatttacgt tggtttaggg
     2281 gcaggctaag tttgtgctgt catcaagggt gaagtcacca gaaataaaac ggttaaccgg
     2341 cgcaccgcca tacagttggc tttgatccgg atcgaccacc acaatatcca ccagataacc
     2401 cggaatacgg acagatttag gatgcagcgt ggctttctta accattttct gcacctgcat
     2461 catcacaata ccgccgttat tgtgcaccgc ctgggcaata accaatgcgt cgagatacat
     2521 cacctcatct tcaaaagtgg cgtagccttc actgtcgcag gtggtagcgc gaatgaaggc
     2581 gatatctggc gcaatcgctt tgtaatagag atattctttg ttatcaaact cgaccagttt
     2641 aatcaggtct tctttcgtga cttcattcag tttgccgcct tgctggcgtg gatcgacaaa
     2701 tgtcccgatg ccaatatcac taataatacc aggctggtgg gctgcggcgg cgcgtaaggt
     2761 ttgtgtaagt acaccttgtg gatagttata agcaataatt ttattttgtt ctgcgagttc
     2821 agaaatacgc ggcgattgtc cccagtgacc acataatgcc catttcacca gaccttcttg
     2881 cgccagagga ctaataccac ggtcggcgcg atcgccaagc cctgtcggac taataatcga
     2941 taaattacgt ggcgtttgag tctgtttata tttatcagca agagctgtaa ttaacgtggt
     3001 ggcttccaga ataccgccgc cagcgcctaa cacacaaagt gttgcttcgt cgggaatata
     3061 attcaccgct tcctgtgccg acaggaccgg cactcgtcca ttaatacgag gtggttttac
     3121 aggtttcatt gttaattcct gattcagatg tgattttccc tacgccagat ggagaattag
     3181 cgcacagaga ctcacgaaat atttttatta aatacggtta aatatatttt ttaagttcgg
     3241 cggggagggt gttcccgccg aaatattatt gcttatgcct ggtgtaaaat agttaatacc
     3301 gtgcgtaaat catttaccga gatttgccct ggtgcagagg ctttttttac cgcaccaaaa
     3361 gttgccgccg agccaaatac ttcaccagcc agacgagaaa ttacgccagt ttttgccatc
     3421 gacatcgtga tgattggacg atcggcatac tgctcctgca tctccagggt cgcggtaagc
     3481 aacgtcagca catcgctggt actttgcggc atcagcgcaa tcttaggaat atcggcgtcg
     3541 aaggactgca ttttgcgcag acgggcaatg atttcttcgg cttccggcgt ttcatggaag
     3601 tcatggttgg acatgacaac tttcacatca tgcgcgtggg cgtaggcgac ggtttcttta
     3661 acctgatcat cgccggtaaa taactccaga tcgatcatat caaccaggcc gctgtcgatg
     3721 gctgcacgat tgagagcaat ataagcctcg gtggaaatcg cctgctcgcc gccttctttg
     3781 gcactgcgga aggtaaacag cagcggtttt tctggcatgg tttcacggag aatttttgcc
     3841 gccgccatga cagactccac attggagagg tcggcaaagt ggtccacacg ccattccaga
     3901 atatcaaagt ccgcttcacg ataggcgaga gcttcggatt tcacgcgggc gatatctttc
     3961 gccatcagcg agacgatgat tttaggtgcg cccgtaccaa tgacgagatc ttttacagtt
     4021 acggttttca ttttttaccc tttctgcacg cggtcagcct gtcaggcacc gaaccccatg
     4081 acctgtttaa catattccag agggaaatct ttgccagtcc ataacgtgaa ctgttcagcc
     4141 ccttgccaca acaacatgcc gtatccatca atcgttttgc aaccagcttg ttgcgcctgc
     4201 tgcaataact tcgtcatatg cgggttatac acgcattcag tgaccagcag tcccggatgt
     4261 aacagactga tatcattaac taatgattca ttctcaaggg gtttcatacc cacttttgtg
     4321 ccattggtta aaatgtcggc ggaagccagg gcttcagcaa aggcttgctg atcggcgaga
     4381 tcggtaaccg tgacgacaca atcggtgttt tcgttaaccc gctgcgcgaa ggcgagggct
     4441 ttatcgaaga actcatcccg acggttaaag agtttaattt cttttaaacc ttcaattgcc
     4501 ccctgcgcgc caatcgccgt tgaggcacct ccggccccta acagcaccat tgttttgcct
     4561 ttgatatcaa aaccgctctc tttaatggcg cgaatatggc ccgtgccgtc ggtgttatag
     4621 ccgcgcaggt agccatcatc attaacgatg gtgttgatgg cacccaccag tttggctgct
     4681 ggcgttaatt catcaacata ttcacacgcc agttgtttgt tcggcattga tataccagtt
     4741 ccgcgcattt tgagggcttt taatccttca attgctccgg gaaaactatc gttatccact
     4801 tcaaaggcca tataggtaaa tggcaatccc gctttttcta aggctttatt ctgcatttca
     4861 ggcgataaac tgtggcggat aggataggcc atcagcccaa tcaattcgta ttttgcggta
     4921 acatccataa ttaactcctt taacctctat gcttaattgt gtttaaccgg gtgctaaatt
     4981 tacgctcgcc aaaccgcaca tcattctcag gaataataaa aacgcggtaa tagcggataa
     5041 aaacaataat tgcggtaatc agggccagca gcgcgaaagt aaaatcgaga acaatgatat
     5101 attgaaggcc gatgttcgac agataaccgg taatcagcgg aataacaaag ttagccagtc
     5161 cacccatcat catataaata ctggtgactt tggctttgct ttttgggaaa aactctgaca
     5221 tgaccgaaac accgagctgt aaaatgccgc cagctgctga gaaaccgata acaaaggcgc
     5281 cggcattaca caccagtgga gaagggtaca ggtagataat ggctgctgtt attgttgcca
     5341 gtgcagagtt aaatacatta gcccagatgg gccggaccat ttttttcagt agtgcggcaa
     5401 aaataaagac acagaccaac gagcccatac tgtaataaga gatggttttt aatgcctcag
     5461 cttctgacat accagcaaaa gccatcgcat atttgggcat ccacaccaca atcacataaa
     5521 aggtcgagaa tgcggctaca ccgaacagta ccgatgaaac accttccagc cagactaacg
     5581 gtttgctgtt catttgcggt aattcattag ctacgctggc gtccaccaac tggctgggga
     5641 atttgctttt caacagcatc agcgtgatca gtacaaacag aatacccgga ataatcagcc
     5701 catagccgta ccagatatta ttgagcaaca tatagctcac cagcattggg tagaacattt
     5761 gcccaaatga caccatcgct ttaaccagta tgaccgccga accagaggct ttcggaaagc
     5821 attccatgag cgcggggtag ccacccgtat ccagcgctga gttagcgata cctacgcaca
     5881 ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg aataccaaag aagaatagca
     5941 gatacattat tactgccatt aatatcaccg cccgacgacc aaacttatcg gagatcacac
     6001 cgaagaataa aatactgatc aatcgcccca aaccaatacc ggaaattaag taggcaatgc
     6061 ccgcgttgtc agtggaaaac ttttccgcca gagatgacat gttttgggca agcgtaataa
     6121 cactaatgcc gtgcaggaag tagctgaagt aaatacaaag aacagccagg ataaatggcg
     6181 tgctgaaagc cttattttga gacattttca cagctccttg ctgaaagata atttcgttac
     6241 cgtacgtatt gttcttcgac ggtttacata attactatac tgaagatgat tttccctggg
     6301 tacatggcat aaagtttatg tttccaggaa tattgcatgt catagtttgt ttttgatcgc
     6361 gattttcctt agttagcgca gaaagagtta tttatccgta agatcttgca tgacctgaaa
     6421 agaatattgc attacccacc cggagcgaca ttttccttta gcgagtgatg ccgcgttttt
     6481 ttgcggctgc gtaagccgat aaacagtgcc agtaaaaaac cgatggcagc gatggcggta
     6541 tcgaaccaca taatatcggc aatacttctt tgggacagat gagccgtaat caacggaata
     6601 gtaaaggtcg caatactgcc cgcactgtaa tagatccctg tagctttacc tttagcgtaa
     6661 gggaaacgtt cagccattaa cgtcaggcca atttgcacaa cacctccagc agaggtaaaa
     6721 ccaatgacaa aagcaaatat tatcaccaca taaaatgtgg gatgcaggca gacggtaaac
     6781 agagcaataa atgagataaa ggtgtacagc atcagtaatg ttgttgggcg aacggtatta
     6841 cgaatgagtg gagcggtaat aaatacacaa agcagcgaac ccacggtgta gatactgagt
     6901 agtttaatcg acatagtgta tgacatgcct gcaacaaatt gttcgtactg tgccagccac
     6961 tggctaacca gataaaacgt tgccattgag atatagccat ataaggtata actggctaaa
     7021 tcgataattg aacagcgatg ttccgtagag ctggtggttt tctttatgac aggtaagcga
     7081 tgacccggat ggggtgggaa cgtacaacgg tataaaaaca gagcgttaat aaacataatg
     7141 cctgcagcaa tcataaagga ccaaccgaac cacagttcag cccacactaa caggctaatg
     7201 attagcggta ataaaaattg tccgctggaa acaaatgctt taattaaaat attggctgtc
     7261 ccaggtgagc gtggaaaagc ttccatcaaa ctgggataag tgcctgcatc gagaaaactg
     7321 tttgccattc ctgccagaaa gccaaaaaca taagcgataa tgatgttatt ggtgtgcagg
     7381 atgccaaaaa agaaggccat atagcagcac atcccgagca tgataaaagg gcggcgacca
     7441 aagcgatcgg ataataatcc tgcaaaaagc aagacactta atcgaccaat gcccagcgat
     7501 gagataacta tcgagacacc cgcggcatta gtctgccaaa gtgtctccag cgaggccata
     7561 ttcaggctca tcaaaatgac gcccatacca tgcaccaggt aattaaaata caacccaagt
     7621 gcggtagggt aatagggatt tttcataggt tctacttagc aaaggtgctt taaaaagtgt
     7681 agcgatgttg tgtcagtatc cgttcattat tgttgtaaca acgacaattc ctttttgtgg
     7741 agaaattaac aaagccgctc cagatcgtgt gcatataatt cagcggctac cgattgatag
     7801 atcttccagt caataaaatt gccatcaggg tagacttgtt gaaacgcggt taagtcgggg
     7861 aaaaaacgca tggtgttgtt gccgatgcgg ttatttattt tctctatgat ggcatgaaga
     7921 tgtttttttc gttgctgacg catagccaac agttgctcga ctacacgatc aggaatggcg
     7981 catttgccat tttcccactg acgccagctt tcactgtcac cggtctgggc aatccaggtc
     8041 gcgcattcat caatagtcat ggcaaaaata tggcgaagtg cctggagttc ataagcgttc
     8101 atcgtgtaat ggctgcatga aataaacaat aaaatataaa taacattatc agcctgctga
     8161 cggcttgaag agagtcacag tatcttgtgc aacattattg gaaagagtga ggggcgaggt
     8221 agcgaagcgg aaaaatgtta aaaaaaagcc catcgtggga gatgggcaaa gactacacac
     8281 agcaattcgt tgtttcactc aggggatttc catgcttata aatcaatatg ttgatttata
     8341 atcgtaagct aatagtaggc atacggactt tttgcgtcga tcagattcgt ctcattagtt
     8401 taaaaactgt aaagaatttg cgacaggtac taccagctga atggcggtaa gcaggaaaat
     8461 atggggaaga gcaagcaaat atcaattagc gggggttaac aatcaggcgg cagagccgcc
     8521 tgatgagaaa ttacttattc ggtttctcga tttcgccaag ttcttcgaga atttcttccg
     8581 gttggtccgt cggcggcggg acttcttcca cccacgcggc aaacagacgc caggaaacgg
     8641 ctaacagaac cggaccaata aacagaccga tcatcccgaa agcaatcaaa ccaccaataa
     8701 caccagagag gatcaggatc agcggtaaat cggcacccat gcgaattaac attgggcgga
     8761 tgacgttatc cagcgtgcca accacaccgc tccacactaa caataccgtt ccccaggtgg
     8821 tatcgccagt ccagtagagc cagataatcg ccggaatcag taccggcaac gggccaagct
     8881 ggacaaggca ggagaggatc attaacaccg ttagcaacgt tgcataaggt acgccggata
     8941 cggcgaggcc gataccgcca agcactgcct gtactaacgc cgtcaccacc acacccagcg
     9001 ccaccgcgcg gatagcctgt gccgccagca gtacggcggc atctccgcga acgcctgcca
     9061 gacgggttgc aaaatggcga atgccttgta ccacctgttc accgcgccag tacagcaggg
     9121 cactgaagag aagcatcagc gcacaatgca ccataaagcg cccgatatgc gccgcctgcc
     9181 caacgaacca ggtggtggtg gtgccaatat aagggcggac tttcgccatg atcgccgtcc
     9241 cccccatatc cagcaagttg tgccagcctg catacagctt cgcgccaatc accggaatgg
     9301 tattaagcca cgccagatcg ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg
     9361 ggccgctgcc gtcgacgata ctgttaacca gcaaagcaat agggatgata aacaccatca
     9421 ctaataacag cgtcatcacc agaacggcga gagagcggcg gccaaacatg atcttttgca
     9481 aacgtaacaa taccggccag gtggcgataa ccaccgtacc ggcccatgca aagccgagaa
     9541 taaagggttg aacaatccac agacatgcca caatcatgat ggctaaaaac agcaccgaaa
     9601 gcagaatttg tgcgacatcc ctgggctgac gaacatttac cataactact tttcaccttt
     9661 tttgtgcgtc aaatcgtcga cgcgacgtga acacgcgaaa ctcacctgca taatgatgag
     9721 caatttcagc ggtttttaac aggccgattc tgcccttaat tctgatgggc gtgttagaaa
     9781 aaaatgtggt acaacaactc aggcaacacg caaacgattt actcgtcgta tttcaagttg
     9841 catgtgctgc ggctacgttc gttcacccca gtcacttact attgtaagct cctggagttc
     9901 actcgcttgc cgccttcctg caactcgaaa tccatagagt aaataaacat cacctttcag
     9961 tgcgctattg cagccgcctg aaagaatagt gtatcgacct acatcacaga ccgcaggaaa
    10021 gggtcaatat aatgattcca cagatttccc aggcacccgg cgtcgttcaa ctggtgctta
    10081 attttttgca agagctggag caacaaggtt ttaccggcga tacggcgaca agttatgccg
    10141 atcgtctgac aatgtcgacc gacaacagta tttaccaact tctccccgat gcggtggtat
    10201 ttccgcgttc aaccgcagat gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt
    10261 catcgctgat ctttaccccc cgtggcggcg gcaccggcac taacggtcag gcgctcaacc
    10321 aggggattat tgttgatatg tcccgccata tgaaccgcat catcgaaatt aaccctgaag
    10381 agggctgggt gcgcgttgag gccggggtga taaaagatca actcaatcag tacctgaaac
    10441 cgttcggcta cttttttgcg ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga
    10501 tgatcaatac cgatgcttct ggtcagggat cgctggtcta tggcaaaacg tcggatcacg
    10561 tacttggcgt acgtgcggtg ttgttggggg gcgatattct cgatacgcaa cctttacccg
    10621 tcgaactggc ggaaacgctg ggtaaatcca ataccacaat cgggcgaatt tataacacgg
    10681 tttatcaacg ttgccgtcag caacgccagt taatcatcga caacttcccc aaacttaacc
    10741 gctttcttac cggttacgat ctgcgtcatg tctttaacga tgagatgacc gagttcgacc
    10801 tgacgcgcat tctgacgggt tcagaaggga cgctggcctt tattaccgaa gcgcggctgg
    10861 atattacgcc cttgcctaaa gtgcgccgtc tggtgaacgt caaatatgac tcttttgact
    10921 ccgcgctgcg taacgcgccg tttatggttg aggcgcgggc gctttcggta gagacggtgg
    10981 actcaaaagt gctgaatctg gcgcgggaag atattgtctg gcattccgtc agcgagttga
    11041 ttaccgatgt gcctgacaaa gagatgctcg ggctgaacat tgtggagttt gctggtgatg
    11101 atgaggcgct gattgacgag cgagttaatg ctctctgtgt gcggcttgat gagctgattg
    11161 ccagccaaca ggcaggtgtg attggctggc aggtgtgccg cgagctggcg ggtgttgaac
    11221 gtatctatgc gatgcgtaaa aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta
    11281 agccaattcc gtttgctgag gatacctgcg taccgccgga acatctggcc gattatattg
    11341 ctgaatttcg cgcgctgctc gacagccacg gcttaagcta cggtatgttc ggtcacgtcg
    11401 acgcaggtgt cttgcacgtc cgtccagcgc tggatatgtg cgatcctcaa caagagattt
    11461 tgatgaagca aatctctgat gacgtagtgg cgctgactgc gaaatacggt ggtttgttgt
    11521 ggggcgagca cggcaaaggt tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac
    11581 tttttgcaga actgcgcaaa gtgaaagcgg catttgaccc gcataaccga ctcaacccag
    11641 ggaagatttg cccgccagaa ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc
    11701 gcggtacatt cgatcggcag atccccattg cggtacgcca gcagtggcgc ggtgcgatgg
    11761 agtgtaacgg caacggttta tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga
    11821 tgaagatcac ccagaaccgg attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat
    11881 ggctgcgttt gttggcggat cgcggcgttg atccactcaa actggaacaa gaactgcctg
    11941 aatccggcgt cagtttgcgg acgttaattg cccgcacgcg caatagctgg catgcgaata
    12001 aaggcgaata tgacttctca cacgaagtca aagaggcgat gtcgggctgt ctggcctgta
    12061 aagcgtgttc gacccagtgc cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc
    12121 tgcagctcta tcacacccgt tatttacgcc cgctgcgcga ccacctcgtc gctacggtcg
    12181 agagctacgc gccgctgatg gcacgcgcgc cgaaaacctt taacttcttc attaaccagc
    12241 cgctggtgcg caaactctcg gaaaaacata tcggcatggt tgatttgccg ctgctgtcgg
    12301 tcccctcgct acaacaacaa atggtggggc atcgctcggc aaacatgacg ctggaacagc
    12361 ttgaatctct caatgcagag cagaaagcgc gcacagtgtt ggtggtgcag gaccccttta
    12421 ccagctatta cgatgcgcaa gtggtggcgg attttgtccg tctggtcgaa aaattaggtt
    12481 tccagcctgt gttactgcca ttttcgccaa atggcaaagc ccagcatatt aaaggttttc
    12541 ttaatcgttt tgcgaagacg gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc
    12601 tgggtatgcc aatggtgggc gtcgatccgg cgctggtact ttgttatcgc gatgaatata
    12661 aactggcgct gggcgaggaa cgtggcgagt ttaacgtctt actggcgaat gaatggctgg
    12721 caagcgcact tgagtcacag ccagtggcta cagtcagcgg tgaatcatgg tatttctttg
    12781 gtcactgtac cgaagttacc gccttgccgg gtgcgccagc acaatgggcc gcgatatttg
    12841 cccgttttgg cgcgaaactg gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt
    12901 acggacatga agcgaaaaac cataaaaatt cgctcgggat ctatgagtta tcctggcatc
    12961 aggctatgca gcgactgccg cgtaaccgct gtctggcgac cggatattcc tgccgtagcc
    13021 aggtaaaacg ggttgaaggc acgggggtac gccatcctgt gcaggcttta ctggagatta
    13081 ttaaatgata tggaaacgga aaatcaccct ggaagcactg aatgctatgg gtgaaggaaa
    13141 catggtgggg ttcctggata ttcgctttga acatattggt gatgacaccc ttgaagcgac
    13201 aatgccagta gactcgcgga caaagcagcc tttcgggttg ctgcatggag gagcatccgt
    13261 ggtactggcc gaaagtatcg gttccgttgc cggttattta tgtaccgaag gtgagcaaaa
    13321 agtggttggt ctggaaatca atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg
    13381 cggggtatgc aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt
    13441 cgatgagaaa gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg
    13501 tcaccagaaa agtgtgacgc atattgcctt tggtcattat ctgcacagta aacggtggta
    13561 tactgtgcag gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca
    13621 gctggccatt gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa
    13681 acgtctgaaa caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt
    13741 aaaagaagaa atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc
    13801 aagttcgcca aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg
    13861 cttatctacc gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc
    13921 gggttagctt tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc
    13981 ggagataagc gccggatggg gtagaaaccc ttaagcctgt gttgcacaga cttaagggtt
    14041 ttcttatttc tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa
    14101 ctttttcttt ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga
    14161 gatacctaat tcttagcgtg cctgttaacc cacacatcag ggtctatgct tattaaaagc
    14221 aggcaaaaag catgttttac ggtaaagccc ctgcgtttgc tgggttgtac tgataatcat
    14281 tatcactaac atgctgttat acgctgaaag cgatgaagtg aggtaaatcg atggacatgc
    14341 attcaggaac ctttaaccca caagatttcg cctggcaagg cttaacgctg acacccgcag
    14401 cggcgataca catccgtgag ctggtggcaa agcagccggg tatggtcggc gtgcgcttag
    14461 gcgtgaagca aacgggctgc gcgggctttg gctatgtgct cgacagtgtt agcgagccgg
    14521 acaaagacga tctgctgttt gaacacgacg gcgcgaagct gtttgtcccg ctgcaagcga
    14581 tgccgtttat tgatggcacg gaagtcgatt tcgttcgtga aggacttaat cagatattca
    14641 aatttcacaa ccctaaagcc cagaatgaat gtggctgtgg cgaaagcttt ggggtatagg
    14701 cggtactatg tctcgtaata ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc
    14761 gctgaattat aaagaaggat tcttcaccca gttagccacc gatgagctgg caaaggggat
    14821 aaacgaagag gtggtgcgcg caatttcggc gaagcgtaat gagccggagt ggatgctgaa
    14881 gtttcgtcta aacgcctatc gcgcatggct ggagatggaa gaaccgcact ggttgaaagc
    14941 gcactacgac aagctgaatt atcaggatta cagctactac tcagcaccat cgtgcggtaa
    15001 ttgtgacgac acttgcgcgt ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt
    15061 tttaagtaaa gaggtggagg cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa
    15121 agaggtggcg gtggatgcca ttttcgactc agtttcggtt gccactactt atcgcgaaaa
    15181 actggcggag cagggaatta ttttctgttc ctttggtgag gcgatccacg atcacccgga
    15241 actggtgcgt aaatatctcg gcaccgtggt gccggggaat gacaacttct ttgccgcgct
    15301 taatgcggcg gtagcctctg acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc
    15361 gatggaactt tccacctatt ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac
    15421 cattctggtg gccgacgaag acagctacgt cagctacatt gaaggctgtt ccgctccggt
    15481 gcgtgacagc tatcagttac acgcggcagt ggtggaagtc atcatccata aaaacgccga
    15541 ggtgaaatat tccacggtac aaaactggtt ccctggcgat aacaacaccg gcggtattct
    15601 caacttcgtc accaagcgtg ctttgtgcga aggcgaaaac agcaaaatgt catggacgca
    15661 atcagaaacc gggtccgcga ttacgtggaa atatcccagc tgcattttgc gcggcgataa
    15721 ctccattggt gagttttact cagtggcgct gaccagcggt catcagcaag cggataccgg
    15781 caccaagatg atccacatcg gtaaaaacac caaatcgacc attatctcga aagggatctc
    15841 tgccggacat agtcagaaca gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa
    15901 tgcgcgcaat ttcactcagt gcgactcaat gctgattggc gctaattgtg gggcgcatac
    15961 cttcccgtat gtcgaatgtc gcaataacag cgcacaactg gagcacgagg caacgacatc
    16021 acgtattggt gaagatcaac tgttttactg cctgcaacgc gggatcagcg aagaagacgc
    16081 catctcgatg attgttaacg gtttctgcaa agacgtgttc tcagaactgc cgctggaatt
    16141 tgccgttgaa gcacaaaaac tcctcgccat cagtcttgaa cacagcgtcg gataaggaat
    16201 aaacatgtta agtattaaag atttacacgt cagcgtggaa gataaagcta tcctgcgtgg
    16261 attaagcctc gccgttcgtc ccggcgaagt tcacgccatt atggggccaa acggttcggg
    16321 caaaagtacc ttatcggcaa cgcttgccgg gcgcgaagat tatgaagtga cgggcggcac
    16381 ggttgagttc aaaggcaaag atttgcttgc actgtcgccg gaagatcgtg caggcgaagg
    16441 catctttatg gccttccagt atccggtgga gattccaggc gtcagtaacc agtttttcct
    16501 gcaaacggca cttaatgcag tgcgcagcta tcgcggccag gaaacgctcg accgctttga
    16561 ttttcaggat ttgatggaag agaaaatcgc tctcctgaag atgccggaag atttattaac
    16621 ccgttcggta aacgttggtt tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat
    16681 ggcggtgctg gaaccggagt tatgcattct tgatgagtcg gactccgggc tggatattga
    16741 cgcattaaaa gtggtcgccg atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat
    16801 cattgttacg cactaccaac gcattctcga ctacatcaag cctgattacg tccatgtgct
    16861 atatcaggga cgaattgtga aatccggcga tttcacgttg gtcaaacaac tggaggagca
    16921 gggttatggc tggcttaccg aacagcagta acgcgctgca acagtggcat cacttgtttg
    16981 aagctgaagg gacaaaacgc tcaccgcaag cacagcagca tttacaacaa ttgctgcgta
    17041 ccggactgcc gacacgtaaa catgaaaact ggaaatatac gccgctggaa gggctgacca
    17101 atagtcagtt tgtcagcatt gcgggagaga tatccccaca gcagcgagat gccttagcgt
    17161 taacgttaga cgctgtgcgg ctggtatttg tcgatggacg ttacgtgtca gcactgagcg
    17221 atgcgactga aggcagcggg tatgaagtga gcattaacga cgaccgtcag ggggtacccg
    17281 acgctattca ggcggaagtg tttctgcatt tgacggaaag cttggcacaa agcgtgacgc
    17341 atatcgccgt gaagcgcggt caacggccgg caaagccatt gctgttaatg catattaccc
    17401 agggcgtggc aggtgaagag gtcaacactg cccattaccg acatcatctg gatctggcgg
    17461 aaggtgccga agcaacggtg atcgaacatt ttgtcagcct taatgatgct cgtcacttca
    17521 ccggcgcacg gttcactatc aatgtcgcag cgaacgccca cttgcagcat atcaagctgg
    17581 cgtttgaaaa cccggtcagt caccactttg cccataacga tttgttgctg gctgacgatg
    17641 ccaccgcatt tagccatagt ttcctgctgg gtggcgcagt gttacgacac aacaccagta
    17701 cgcaactcaa tggcgaaaac agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa
    17761 acgaggtgtg tgatacccgt acctggctgg aacacaataa aggtttttgt aacagccgac
    17821 agttgcacaa aactatcgtc agcgacaaag gccgcgcggt atttaacggt ttgatcaacg
    17881 tcgcgcagca cgccatcaaa acggatggtc agatgaccaa caataatctg ctgatgggca
    17941 aactggcaga agtggatacg aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca
    18001 gccacggcgc gacggtgggg cgtattgatg atgaacagat gttctatctg cgctcgcgcg
    18061 ggatcaatca gcaggatgcc cagcagatga tcatttacgc ctttgctgcc gaactgacgg
    18121 aagcactgcg tgatgagggg cttaaacagc aggtgctggc ccgaatcggt caacggctgc
    18181 caggaggtgc aagatgactt tttccgtcga caaagtgcgg gccgactttc cggtgctttc
    18241 tcgtgaggta aacggtttgc cgctggctta tctcgacagc gccgccagtg cgcagaaacc
    18301 gagccaggtg attgacgccg aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg
    18361 cggtattcat accttaagcg cccaagcgac cgagaaaatg gagaacgtgc gcaagcgggc
    18421 atcgctgttt attaatgccc gttcggcgga agagctggtg ttcgtccgcg gcacgacgga
    18481 agggatcaat ctggtcgcca atagctgggg taacagcaat gtgcgggcgg gcgataacat
    18541 catcatcagt cagatggagc accacgctaa cattgttccc tggcagatgc tttgcgcacg
    18601 cgttggcgca gagctgcgtg tgatcccgct caatcccgac ggtacgttgc aactggagac
    18661 gctgcctacg ctgtttgatg agaaaactcg cctgctggca attactcatg tctccaacgt
    18721 gcttggcaca gaaaatccac tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa
    18781 agtgctggtg gatggcgctc aggcggtgat gcatcatccg gtggatgttc aggcgctgga
    18841 ttgcgacttt tacgtgttct ccgggcataa actgtatggc cccaccggaa ttggcattct
    18901 ttatgtgaaa gaagccttgt tgcaggagat gccgccgtgg gaagggggcg gttctatgat
    18961 cgccaccgtc agcctgagtg aaggcacaac ctggaccaaa gcaccttggc ggtttgaagc
    19021 cggtacaccc aataccgggg gcatcattgg tcttggcgcg gcgctggagt atgtttcggc
    19081 gctggggctt aataacatag ccgagtatga acagaatctg atgcactacg cgctatcaca
    19141 gctggaatct gtaccggatc tcactctcta tggtccgcag aacaggcttg gcgttattgc
    19201 ttttaatctt ggtaaacacc acgcctatga tgttggcagt tttctcgata actacggcat
    19261 tgctgtgcgt accggacatc actgcgctat gccattaatg gcctattaca acgtccctgc
    19321 tatgtgtcgg gcgtcgctgg ccatgtataa cacccatgaa gaagtggatc gtctggtgac
    19381 cggactgcaa cgtattcacc gtctgctggg ataacaggga ggcactatgg ctttattgcc
    19441 ggataaagaa aaattgctgc gtaatttttt acgctgcgcc aattgggaag agaaatatct
    19501 ctacattatt gagctgggcc agcgtctgcc agaattacgc gacgaagaca gaagcccaca
    19561 aaatagcatt cagggctgcc agagtcaggt gtggattgtc atgcgccaga atgcgcaggg
    19621 aattattgaa ttgcagggcg acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt
    19681 ctttattctc tacgatcaga tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg
    19741 gtttgaaaaa atggcgctca cccaacatct caccccatct cgttcacaag gtctggaagc
    19801 gatgattcgc gcaattcgcg ccaaagccgc tgcacttagc taaactaaag ggacagcttt
    19861 catccggttg gctcacattg ccggatggtc aatgtttaca ctgacctgca caggagtgtt
    19921 tcggtttcag gtgaacataa gcgccatccg acgtttcagc gtgagtctcc ggcaaataca
    19981 ggaggtttac aatgaaacgc gcgtctttgc ttacactcac gcttatcggc gcttttagcg
    20041 ccatccaggc tgcctgggcg gttgattatc cgctaccacc aaccggaagc cgactggttg
    20101 ggcaaaatca aacgtatacg gtgcaggaag gggataaaaa ccttcaggcc atcgcccgac
    20161 gttttgatac tgcggcaatg ttgatccttg aagccaataa cactatcgcc ccggtgccaa
    20221 aacccggtac gacgataact attccttcgc aactgttatt acctgatgcg ccgcgtcagg
    20281 ggattatcgt taaccttgca gagctgcgcc tttattatta tccgccggga gaaaatatcg
    20341 tgcaggttta tccgataggt attggcttgc aggggctgga aacgccggtg atggaaacgc
    20401 gtgtggggca gaaaatccct aacccaacct ggacgcctac tgcaggcatt cgtcaacgtt
    20461 cgctggagcg tggtattaaa ttaccgccag tcgttcctgc cggaccaaat aacccgctag
    20521 gacgttacgc actgcgcctc gcgcatggta atggcgaata cctcattcat ggcaccagtg
    20581 cgccggacag cgtcggtttg cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata
    20641 ttaaagcctt gttctccagc gtgcggacgg gaacgccggt gaaagtgatc aacgaacctg
    20701 tgaaatattc cgtggagcct aacgggatgc gttatgttga agtacatcga ccactatcgg
    20761 cagaagaaca gcagaacgtt cagacaatgc catacacact gccagcaggc tttacgcaat
    20821 ttaaagacaa taaggctgta gatcagaagt tagtcgataa agcgttgtat cgtcgggcag
    20881 ggtatccggt ttcggtgagc agtggagcaa ctcccgcagc cagcaatgcg ccttcagtag
    20941 agtcagcgca gaatggtgaa ccagagcaag ggaatatgtt acgcgcgacg cagtagcggt
    21001 aaacggcaga caaaaaaaat ggcgcacaat gtgcgccatt tttcacttca caggtactat
    21061 tacttgcggt atttagtagc catgttgtcc agacgctggt tagcacgagc tgcgtcatct
    21121 ttagcagcct gaacgtcgga acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta
    21181 gcgttcagag tctgaacgtc agaagacagc tgatcgattt tagcgttgct ggagcaacct
    21241 gccagcagag tagaacccag gattaccgcg cccagtacca gtttagtagc tttcattatt
    21301 aataccctct agattgagtt aatctccatg tagcgttaca agtattacac aaagtttttt
    21361 atgttgagaa tatttttttg atgggaaggc acttattttt gatcgttcgc tcaaagaagc
    21421 atcgaacgct gggttttaga taaaaaaatt gagagaaa
//
LOCUS       DAZGTR010000074        21339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGTR010000074 DAZGTR010000000
VERSION     DAZGTR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.074"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..262
                     /locus_tag="ACHLCY_003076"
     CDS             <1..262
                     /locus_tag="ACHLCY_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFP0370285.1"
                     /translation="GLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKN
                     LAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
     gene            559..765
                     /locus_tag="ACHLCY_003077"
     CDS             559..765
                     /locus_tag="ACHLCY_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370286.1"
                     /translation="MEGVVSQEQAGHRASKNNRFVLFFSQGKNSTGINPSSGGISRQH
                     DEQAAQPDTASRRYYDFRNTFSKA"
     gene            1758..2582
                     /gene="dinD"
                     /locus_tag="ACHLCY_003078"
     CDS             1758..2582
                     /gene="dinD"
                     /locus_tag="ACHLCY_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP0370287.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            2874..3491
                     /gene="yicG"
                     /locus_tag="ACHLCY_003079"
     CDS             2874..3491
                     /gene="yicG"
                     /locus_tag="ACHLCY_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP0370288.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(3488..5170)
                     /gene="ligB"
                     /locus_tag="ACHLCY_003080"
     CDS             complement(3488..5170)
                     /gene="ligB"
                     /locus_tag="ACHLCY_003080"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP0370289.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            5428..6051
                     /gene="gmk"
                     /locus_tag="ACHLCY_003081"
     CDS             5428..6051
                     /gene="gmk"
                     /locus_tag="ACHLCY_003081"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP0370290.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            6106..6381
                     /gene="rpoZ"
                     /locus_tag="ACHLCY_003082"
     CDS             6106..6381
                     /gene="rpoZ"
                     /locus_tag="ACHLCY_003082"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP0370291.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            6400..8508
                     /gene="spoT"
                     /locus_tag="ACHLCY_003083"
     CDS             6400..8508
                     /gene="spoT"
                     /locus_tag="ACHLCY_003083"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP0370292.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            8515..9204
                     /gene="trmH"
                     /locus_tag="ACHLCY_003084"
     CDS             8515..9204
                     /gene="trmH"
                     /locus_tag="ACHLCY_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP0370293.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            9210..11291
                     /gene="recG"
                     /locus_tag="ACHLCY_003085"
     CDS             9210..11291
                     /gene="recG"
                     /locus_tag="ACHLCY_003085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP0370294.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVHHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(11276..12142)
                     /locus_tag="ACHLCY_003086"
     CDS             complement(11276..12142)
                     /locus_tag="ACHLCY_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370295.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(12145..13350)
                     /gene="gltS"
                     /locus_tag="ACHLCY_003087"
     CDS             complement(12145..13350)
                     /gene="gltS"
                     /locus_tag="ACHLCY_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP0370296.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            13630..15021
                     /gene="xanP"
                     /locus_tag="ACHLCY_003088"
     CDS             13630..15021
                     /gene="xanP"
                     /locus_tag="ACHLCY_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP0370297.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            15142..16851
                     /gene="yicH"
                     /locus_tag="ACHLCY_003089"
     CDS             15142..16851
                     /gene="yicH"
                     /locus_tag="ACHLCY_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0370298.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDVAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(16904..19222)
                     /gene="yicI"
                     /locus_tag="ACHLCY_003090"
     CDS             complement(16904..19222)
                     /gene="yicI"
                     /locus_tag="ACHLCY_003090"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP0370299.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(19232..20614)
                     /gene="yicJ"
                     /locus_tag="ACHLCY_003091"
     CDS             complement(19232..20614)
                     /gene="yicJ"
                     /locus_tag="ACHLCY_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP0370300.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            20907..21001
                     /locus_tag="ACHLCY_003092"
     tRNA            20907..21001
                     /locus_tag="ACHLCY_003092"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20941..20943,aa:Sec,seq:tca)
     gene            21080..21208
                     /gene="rhuM"
                     /locus_tag="ACHLCY_003093"
                     /pseudo
     CDS             21080..21208
                     /gene="rhuM"
                     /locus_tag="ACHLCY_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         5383 a         5721 c         5356 g         4879 t
ORIGIN      
        1 gggacttgaa ccgaagaaca cggcggtgcg gagtccggag agtaacggaa tagcagagag
       61 cttcgtgaaa acgataaagc gtgactacat cagtatcatg cccaaaccag acgggttaac
      121 ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc
      181 gctgggttat cgctcgccac gggaatatct gcggcagcgg gcttgtaatg ggttaagtga
      241 taacagatgt ctggaaatat aggggcaaat ccaatcatga aaaacgtaat cgcctgataa
      301 ttatgccacg aagcacggaa aaacagtact ggtgctaaga catgaaccat ttgcattgca
      361 ataagttaag ccgaaatgcg tcgggtcctg aatggtgata atcaccacgc agacaatcaa
      421 agctaccata acgaacatcg ttacagttgg agcttttacc ccgttgggga aaaggtgcac
      481 atctggatca tcattatggt gatcgagggt atgtaaagcc caccagcctg attaaaggtt
      541 aaccggaaaa agccaggtat ggagggagtg gttagccaag aacaagccgg acaccgcgcc
      601 agtaaaaaca acagattcgt gcttttcttc agccagggta agaactccac ggggattaat
      661 ccgtcgtctg gtggaatcag tcggcagcat gacgaacagg ccgcgcagcc ggacaccgcc
      721 agcagaaggt attatgattt tcgtaatacc tttagcaaag cgtgaatcat cattacggta
      781 gggatggtca taatgaccac acctcccccc caccggaaca gggaggatga cctacgaaaa
      841 tttcgtaaaa ttccccccca aatcccccac ggttgcccgt taagtgcaag agcacaggaa
      901 cgacctcgct aatcagttac cagcgctcac gctcaaatat ccacaggaac ggggctaaat
      961 cgcattctgg cgcaatgttc actatcgggg caagcattca ccggaaaacg tcatacaggc
     1021 ttacaggcgg cttaaaattc tatgggcgga taacagggcg ggatcgggag gcttacgaaa
     1081 atttcgtaac ccccctacac aaaaagtgca ggttactacc cgaaaagtgt agtgccattg
     1141 ctgggggcta aaaaaaaccg cagtgtgata acactacggc acgccaccaa agaaacataa
     1201 caaggaatca gcaatgagta atacctgtaa gccttcgggg tgattgtaac acatgagcaa
     1261 tcttttactg gaagctgttt ttcactggat cacgtatcgt tatggggatt cccatatcgg
     1321 agagcatcca ccagcttcat aacggataac cgaaaaatga taacggttaa cctggaaaaa
     1381 attccggtga atatttgccc tgacagttcc catatcggcg gcacacctga aaaaaccgtg
     1441 tacacaagga tgtacacaat aaaaatcgtt attacagaaa tcccagtaat gacaagcctt
     1501 tgatgcagat actcaatgag cagattcaat tgagtaactc gtaacactaa ttcttacttt
     1561 tcagccttca caaatgccgc ccactcaaac agagcggcat ttttcttccc cgcaacttca
     1621 attctgtttt gcgtgcctgc tccagatttt gcgatgtttt tttgcccagc acactgagaa
     1681 cgtgagatac tcacaactgt atataaatac agttacagat ttactttctt tgcaatcgat
     1741 atcacatgga gtgggcaatg aacgaacatc atcaaccttt tgaagaaata cggcattatg
     1801 gcactgaagg gcaagaattt tggtcagcta gagaattggc accgttactg gattatcggg
     1861 actggcgcaa ttttcaaaag gtactggccc gcgcgactca agcgtgcgaa gccagcaatc
     1921 aagctgcatc tgaccatttc gttgaaacca ccaaaatggt tgtattaggt tcaggggctc
     1981 aacgagaact tgaagatgtt catctctccc gctatgcctg ttacctggta gtacaaaacg
     2041 gcgaccctgc gaaaccggtt attgcggcag ggcaaactta ttttgctatc cagacccgac
     2101 ggcaggagct ggctgacgat gaagctttca ggcaacttcg tgaagacgaa aagcgtcttt
     2161 ttctgagaaa tgaattgaaa gaacataaca aacaattggt tgaggcagcg cagcaagctg
     2221 gggtagcgac agctatcgat ttcgccatat ttcagaatca tggttaccgg gggctgtatg
     2281 gtggattaga tcagaaagcc atccatcagc ggaaggggct gaaaaagagt cagaagatcc
     2341 tggatcatat gggttcaaca gaactggcgg ctaatctctt tcgagctacc caaacagaag
     2401 aaaaactcaa gcgggatggc gtgaattcaa aacagcaagc taataccaca cactttgacg
     2461 tgggtagcaa ggtgaggcaa accattcagg aacttggcgg aaccatgcct gaggagttgc
     2521 cgaccccgca ggtcagcatc aagcagttgg aaaacagtgt aaaaattaca gagaagaaat
     2581 agcagaaact aaaaactctt tttgttgatt gagatacccg atgcgtaagc caaagtccag
     2641 gtgcggtgaa cacaatggct aaacattgca cctttctttt ccacccctca attttaacct
     2701 attttttctt atgcattttc tcagacaaga agtcagaaga atgcatctac gctacagaaa
     2761 atagcgattt cacataacta caagttatca atttcctctc accttaaaaa atctcaatcg
     2821 tgacaatacg cacaaatcgc taccctgcca gacagatttt tagggagaga accatgctgt
     2881 tacacatttt gtatttggtt ggcattactg ccgaagccat gacgggggct ctggcggccg
     2941 ggcgacgccg catggataca tttggcgtaa ttattattgc gaccgccacc gcaattggcg
     3001 gagggtcagt gcgcgatatt ctgctgggcc actatccgct cggctgggtc aaacacccgg
     3061 aatatgtgat tatcgtcgct accgccgcag tactcaccac tatcgttgct cctgtaatgc
     3121 cttacctgcg caaagtgttt ctggtgctcg atgcgcttgg actggtggtc ttttctatca
     3181 tcggcgcaca ggttgcactg gatatggggc acggcccaat tattgccgtt gtcgcggcgg
     3241 taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt ctgtaaacgt atcccactgg
     3301 tattccagaa agagttgtat gccggtgtct catttgcctc cgccgtgctg tacatcgcgc
     3361 tgcaacacta tgtttctaac catgatgtgg taattatctc caccctggta ttcggctttt
     3421 ttgcccgctt actggcgcta cgccttaagc tgggattacc ggtcttttac tacagccacg
     3481 aaggacacta aggttcaaaa cctgtgatct gctgggcagc cagccaactg cccagcttct
     3541 tgatttgcgc attttccttc cattcaataa cctgtctggc gcgccccgat ccagtccccg
     3601 gcagctgctg ccagaactgc tccgtgctaa ataaaagttg cgaccaggac cgttcatcac
     3661 tggcattaag cgccgcccgg gttagcggta ttcccattgc catcacccag cgagtaaaag
     3721 gctgcttacg agccagatta aaccgatgcc atagctgcgc acttttactt ttcgcgatcc
     3781 ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca ggaaaagata tgctcaaagc
     3841 gatgagtctg atgcagcgcg cgccagccgg cctcaccaat accatccagc cccagaacct
     3901 gttttgaccc cagccagact aagcgtgaaa tgaactgttc ctgacaaaca tcagaagcaa
     3961 agtagcaggt caacgagtta aagcggtttt ctggtggtgt cggttttgta cgttctgcac
     4021 cgcgccagac cacatcatca atgcgaggaa taccctgacc ggcaaggctg acgaggatct
     4081 gatcaccagg cgcaatatcc cactcctgcc agcgcctgac ggaaccaata ttcacccgct
     4141 ggactttttt atcatccagc atgacaggtg cgagtgacgc aaccaccgat attttaccgc
     4201 tcttacccac cgcaaactga atcgccttca cttcggcaac ctgagctaca ggttgatatt
     4261 tccaggccac cagccattct gcctggcctg gtagccaatg gcgggattcg ggttctttcg
     4321 cccctcgcac aaccacgcca tcggtgacga agggtaattt cgctttccac cactcattgc
     4381 gtacgcgcgc aacctcatca gcatttttaa ccgcacgggt atacctctgc gttagagtaa
     4441 aacctgcggt agccagctcc tttaaacgat cggtcattaa ctgcggtcca tccggccatg
     4501 cccagacaaa aacgcccaga gaattcagcg tgtcgctatc gtcctggcgc atcatcaggc
     4561 cagcaacttt tgcgcgggca tttattcccc ccatttgttg ttggatatgc ccctcgcgct
     4621 ggagaaatat ttccccctga agcgtactgt tggctaaagg cccgctaacg gtttgcggca
     4681 cagcggaaat taagctaact ttctgcgtcc agtcctcgcc tttcaggcca ttaccgcgac
     4741 tgattgcttt gttcagtttc ccgtcccgat aaaccagggt taccgcaacg ccatcaactt
     4801 ttggctgcac ccaaagatcg ctacgttctc gcatccacag acttaatgcg tttttatccg
     4861 ccattttgcg tacacccgta tgggcaaccg gatgcataac cgcaccattt aaaggcggca
     4921 tcatcacgtc gcggggctcg ctcacaaagc agcgttgcca ctgcgtaaga cgggcgctta
     4981 actgatcgta aacaccgtct tccacctcac tttttccttc cttccagtag tcatcgtccc
     5041 actgttttat ttgctgttgc aggcgggaaa tttcttcctg tgctctggct ggcgaccagg
     5101 ccggacagac cgcccacaca gatgattgcc agcacaagat acttattaat atcgccatcc
     5161 atactttcat cttcacctcc gctgtagtca ggctggcaga tatacaacgt ggtgaaggca
     5221 aagccgagtg gcaaaaacgg agtctgcgag gacgcttcct gagaatcgtc tttattgcag
     5281 taaatcacag gcaaatgcgg aaacagctac gcaaaacgca acaactttgc gcaaaaagtg
     5341 tgagcaaggg ctacgtcaca tggccgcgcc gtgtataata agctcgtatg taggctttat
     5401 ttcgctaatc acatacgaaa gatactcatg gctcaaggca cgctttatat tgtttctgcc
     5461 cccagtggcg cgggtaaatc cagcctgatt caggctttat taaaaaccca accgttgtat
     5521 gacacccagg tttctgtttc acacaccaca cgccaaccgc gtcctggtga agtccacggt
     5581 gaacattatt tctttgttaa tcatgatgaa tttaaagaaa tgattagcag agatgcgttc
     5641 ctcgaacacg cagaagtttt tggtaattac tatggcactt cgcgtgaggc cattgagcaa
     5701 gtactggcga ccggtgtcga tgtttttctc gatatcgact ggcagggcgc gcagcaaatt
     5761 cgccagaaga tgccgcacgc gcggagtatc tttattttac cgccgtccaa aattgaactg
     5821 gaccgccgtc tacgcggtcg cggtcaggac agcgaagagg tcattgcaaa gcgtatggcg
     5881 caagctgttg cagaaatgag ccattacgcc gaatatgatt atctgattgt gaatgatgac
     5941 ttcgataccg cgttgaccga tttgaagacc attattcgcg ccgaacgtct gcgcatgagc
     6001 cgccaaaagc agcgtcatga cgctttaatc agcaaattgt tggcagactg aacctgattt
     6061 cagtatcatg cccagtcatt tcttcacctg tggagctttt taagtatggc acgcgtaact
     6121 gttcaggacg ctgtagagaa aattggtaac cgttttgacc tggtactggt cgccgcgcgt
     6181 cgcgctcgtc agatgcaggt aggcggaaag gatccgctgg taccggaaga aaacgataaa
     6241 accactgtaa tcgcgctgcg cgaaatcgaa gaaggtctga tcaacaacca gatcctcgac
     6301 gttcgcgaac gccaggaaca gcaagagcag gaagccgctg aattacaagc cgttaccgct
     6361 attgctgaag gtcgtcgtta atcacaaagc gggtcgccct tgtatctgtt tgaaagcctg
     6421 aatcaactga ttcaaaccta cctgccggaa gaccaaatca agcgtctgcg gcaggcgtat
     6481 ctcgttgcac gtgatgctca cgaggggcaa acacgttcaa gcggtgaacc ctatatcacg
     6541 cacccggtag cggttgcctg cattctggcc gagatgaaac tcgactatga aacgctgatg
     6601 gcggcgctgc tgcatgacgt gattgaagat actcccgcca cctaccagga tatggaacag
     6661 ctttttggta aaagcgtcgc cgagctggta gagggggtgt cgaaacttga taaactcaag
     6721 ttccgtgata agaaagaggc gcaggccgaa aactttcgca agatgattat ggcaatggtg
     6781 caggatatcc gcgtcatcct catcaaactt gccgaccgta cccacaacat gcgcacgctg
     6841 ggctcacttc gcccggacaa acgtcgccgc atcgcccgtg aaactctcga aatttacagc
     6901 ccgctggcgc accgtttagg tatccaccac attaaaaccg aactcgaaga gctgggtttt
     6961 gaggcgctgt atcccaatcg ttaccgcgta attaaagaag tggtgaaagc cgcgcgcggc
     7021 aaccgtaaag agatgatcca aaaaatcctc tctgaaatcg aagggcgttt gcaggaagcg
     7081 ggaattccgt gccgcgtcag tggtcgcgag aagcatcttt attcgattta ctgcaaaatg
     7141 gtgctcaaag agcagcgttt tcactcaatc atggacatct acgctttccg cgtgatcgtc
     7201 aatgattccg acacctgtta tcgcgtgctc gggcagatgc atagcctgta caagccgcgt
     7261 ccgggtcgcg tgaaagacta catcgccatt ccaaaagcga acggctatca gtctttgcac
     7321 acctcgatga tcggcccgca cggtgtgccg gttgaggtcc agatccgtac cgaagatatg
     7381 gatcagatgg cggagatggg tgttgccgcg cactgggctt ataaagagca cggcgaaacc
     7441 agcaccaccg cgcaaatccg cgcccagcgc tggatgcaaa gcctgctgga gctgcaacag
     7501 agcgccggta gttcgtttga atttatcgag agcgttaaat ccgatctctt cccggatgag
     7561 atttacgttt tcacaccgga agggcgcatt gtcgagctgc ctgccggtgc aacgcccgtc
     7621 gacttcgctt atgcagtgca taccgatatc ggccatgcct gcgtgggcgc acgcgtcgac
     7681 cgccagcctt acccgctgtc gcagccgctt actagcggtc agaccgttga aatcattacc
     7741 gctccgggcg ctcgcccgaa tgccgcttgg ctgaactttg tcgttagctc gaaagcgcgc
     7801 gccaaaattc gtcagttgct gaaaaacctc aagcgtgatg attctgtaag cctgggccgt
     7861 cgtctgctca accatgcttt gggtggtagc cgtaagctca atgaaatccc gcaggaaaat
     7921 attcagcgcg agctggatcg catgaagctg gcaacgcttg acgatctgct ggcagaaatc
     7981 ggccttggta acgcaatgag cgtggtggtc gcgaaaaatc tacaacatgg ggacgcctcc
     8041 attccaccgg cgactcaaag ccacgggcat ctgccgatca aaggcgctga tggcgtgctg
     8101 atcacctttg cgaaatgctg ccgccccatt cctggcgacc cgattatcgc ccacgtcagc
     8161 cccggtaaag gtctggtgat ccaccatgaa tcctgccgta acatccgtgg ctaccagaaa
     8221 gagccagaga agtttatggc tgtggaatgg gataaagaga cggcgcagga gttcatcacc
     8281 gaaatcaagg tggagatgtt caatcatcag ggtgcgctgg caaacctgac ggcggcaatt
     8341 aacaccacga cctcgaatat tcaaagtttg aatacggaag agaaagatgg tcgcgtctac
     8401 agcgccttta ttcgtctgac cgctcgtgac cgtgtgcatc tggcgaatat catgcgcaaa
     8461 atccgcgtga tgccagacgt gattaaagtc acccgaaacc gaaattaatg ttttatgaac
     8521 ccaacacgtt atgcacgcat ctgcgaaatg ctcgccaggc ggcagcctga tctgaccgtc
     8581 tgcatggagc aggtccacaa acctcataac gtttctgcga ttattcgtac cgcagatgcc
     8641 gttggcgtac atgaagttca cgccgtctgg cctggtagcc gcatgcgcac catggcttcg
     8701 gcagcggcgg gtagtaacag ctgggtacag gtgaaaacac accgcaccat tggcgatgcc
     8761 gtcgctcatc tcaaaggcca gggcatgcag attctggcaa cccatctttc tgataacgct
     8821 gtcgatttcc gcgaaattga ttacactcgc ccgacctgca ttttgatggg acaggagaaa
     8881 acgggcatca cgcaggaagc attggccctg gcggatcagg acatcatcat tccgatgatc
     8941 ggcatggtgc agtcgctgaa tgtgtccgtt gcctcagccc tcattcttta cgaagcccag
     9001 cgtcagcggc aaaatgcagg catgtacctg cgtgaaaaca gcatgttgcc ggaagcagag
     9061 caacaacgcc tgttgtttga aggcggctat ccggtgctgg cgaaagtcgc aaaacgcaaa
     9121 ggcctgcctt atccccacgt caatcagcaa ggcgagatcg aagctgatgc cgactggtgg
     9181 gctactatgc aggctgcagg gtaagtgcca tgaaaggtcg cctgttagat gctgtcccac
     9241 tcagttccct aacgggcgtt ggcgcagcac ttagtaacaa actggcgaaa atcaacctgc
     9301 ataccgtgca ggatctactc ttacaccttc ccctgcgcta cgaagatcgc acccatctct
     9361 accccatcgg agaactactg ccgggcgttt atgccacggt ggaaggcgaa gtgctgaact
     9421 gcaatatctc cttcggcggt cggcggatga tgacctgcca gatcagcgac ggttccggca
     9481 tcctcaccat gcgctttttc aatttcagcg cggcaatgaa aaatagcctg gcggcgggcc
     9541 gccgtgtact ggcttatggc gaagcaaagc gcggtaaata tggtgcggag atgatccacc
     9601 cggaataccg cgtgcagggc gatctcagca cgccagaatt acaggaaacg ctcacgccgg
     9661 tttatccaac aacggaaggc gtaaagcagg ccacgctgcg taaattaacc gaccaggcgc
     9721 tggatctgct cgacacctgt gccattgaag aactcctgcc gccggaactg tcacaaggaa
     9781 tgatgacgct accggaagcg ttgcgcactt tgcaccgccc gccaccgacg ctacagctta
     9841 gcgatctgga aaccgggcag catccggcgc aacgtcgtct gattctggaa gaactgctgg
     9901 cgcacaacct cagcatgtta gccttacgtg ccggagcaca gcgttttcat gcccagccgc
     9961 tgagcgccaa tgacacgctg aaaaataaac tcctcgccgc cttaccgttc aagccaacgg
    10021 gcgcacaggc acgcgtagtg gcggagatcg agcgcgatat ggcgctggat gtgccgatga
    10081 tgcgtctggt gcagggcgat gtaggttccg gtaaaacgct ggtcgccgcc ctcgccgcgt
    10141 tgcgtgcgat tgcccacggc aaacaggtag cattgatggc accaaccgaa ttactcgccg
    10201 agcagcacgc caataacttc cgcaactggt ttgcaccgct cggtatcgaa gtgggctggc
    10261 tcgccggtaa gcagaaaggt aaagcacggc tggcacagca ggaagccatc gccagcggtc
    10321 aggtgcagat gattgtcggt acacacgcca tcttccagga acaggtgcag tttaacggcc
    10381 tggcgctggt gattatcgac gaacagcatc gttttggcgt gcatcagcgt ctggcattgt
    10441 gggagaaagg ccagcagcag ggcttccatc cgcatcagtt gatcatgacc gccacgccga
    10501 tcccccgcac gctggcaatg actgcgtatg ccgatctcga tacctcggtg atagatgagc
    10561 tgccgccagg ccgcacgcca gtgactacgg tcgctattcc tgatacccgc cgtaccgaca
    10621 tcattgaccg cgtgcaccac gcctgcataa ctgaaggtcg tcaggcatac tgggtttgta
    10681 cgttgattga agagtcggaa ttgctggaag cgcaggcggc ggaagctacc tgggaagagt
    10741 tgaaactggc gctaccagag ttgaacgttg gcctggtaca cgggcggatg aaacctgccg
    10801 agaaacaggc ggtgatggcg tcgtttaaac aaggtgagct acacctgctg gttgccacaa
    10861 ccgttattga agtcggcgtt gatgtgccta acgccagtct gatgattatc gaaaacccgg
    10921 agcgtctggg tctggcgcag ttacaccagc ttcgcgggcg tgtaggtcgt ggtgcggtgg
    10981 cttctcactg cgtgctgctc tacaaaacgc cgctctccaa aaccgcgcaa attcggctgc
    11041 aagtgctgcg cgacagcaac gacggttttg tgattgcaca gaaagactta gagattcgcg
    11101 gaccgggtga actgttaggt acgcgtcaga cgggtaatgc tgagtttaaa gtggcggatt
    11161 tactgcgcga tcaggcgatg atcccggaag ttcagcgcct ggcgcgacat attcacgaac
    11221 gttacccaca acaagcaaaa gccctgatag aacgctggat gccggagacg gaacgttact
    11281 ccaacgcatg accagactca tcacaagaca agtcacgcag taaatcttca atctggatag
    11341 tgaggatatc cattgcctca tacgcatcct gtgcaattcg cgccgccatt gcgccttccg
    11401 gttcaccacg cagaatttca gccgctgcct gtacgccagc cacaatcatt tcaggtgtta
    11461 acatttggcg aagaaaagcg cgattatcag aggaccagcc atctttatgg cctaatggat
    11521 gcccccagcc tatccattct tgcgaccata acggattggc ttctatggga taaattagat
    11581 gcgcggcata ctgaatagca cacaccgctt ttcctggcgt atccagcgcc caccagcgcg
    11641 accagagaga acgaattaac ggcgcggccc cacccagagt attgaacacc gccagccagc
    11701 aaagaacgtt attgaaacca cgctcgttat ccatacgctg caacgcggtg tccagtaaaa
    11761 attgccgtac ctgctggcgt tgggaagcat tcatcatctc ctgccagaga tagggccgac
    11821 acaatgcctt cattaaggat tcttcgaagt gatcaccata accgccagct aaaacggttt
    11881 catgccattg cgccaggcag agcggaaaca gatagcgaaa caactctatt tgcatcggtt
    11941 cgttataaac gagattgagt acatagtaag cactcaaatc ccattcgctg atctgctgcc
    12001 actctttagc cgccagcgcc ttgagttcgg catctgcgta atcaaattcc gtttcccata
    12061 cacagagcac tggcggctca tcaccaaaaa tatggcgaca gtgttcacgt agcgtttccg
    12121 gttccttccg acgctttttc atttttaacc ggcaaaaatc ggcaacatta aatacagctt
    12181 aatcaccagc gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa
    12241 cgccatgtgc gacgggccaa agcgttcagt gatcgcctgc atgttggcga ttgccgttgg
    12301 cgttgcaccg aggccaaaac cacagtgacc cgcagccagc actgccgcat cgtagttttt
    12361 gcccatcatg cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac
    12421 cagaatcgcc agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcag
    12481 cgccatcgcc aggaacaacg acaggcttac gttacccagc acggataccg cacgctcaaa
    12541 gacgcggtaa aagcccatca ttgacagacc gttgctcaga atcacgccaa caaacagtac
    12601 acagacgaag gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc
    12661 caccgtcagg cagatagcaa tcagcgcgat agtttcaatc agcaccagcg aggtgatcat
    12721 gcgtcccaca tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt
    12781 cggcgtggtg gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag
    12841 caccagaccg aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg
    12901 ttcaatgaac aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga
    12961 accggccaac agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat
    13021 ggcattttgc atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc
    13081 accggcacgc aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat
    13141 taacggatcg cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac
    13201 tagtagcgcc agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt
    13261 caaaaaggag acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac
    13321 aagcgttgct aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt
    13381 catactcgtc atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt
    13441 atgctttcct gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga
    13501 aaaataccaa aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat
    13561 gtggcaaacg tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga
    13621 ttgttttcga tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact
    13681 caaaacagcg aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt
    13741 gccgcctgtc agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc
    13801 tgccaggcgc tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt
    13861 gcctccggtg tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg
    13921 ttgtctattc agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg
    13981 ctgaaaaccg gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg
    14041 ctggcaagtt gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt
    14101 acgccgctgg tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggttggg
    14161 ttaacgtcca ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa
    14221 aatctgctgc tggcaggcgt ggtcttagcc ttaattatcc tgcttaaccg tcaacgtaac
    14281 ccttacttac gcgtggcctc actggtgatt gcgatggcgg ccggatatgc gctggcgtgg
    14341 tttatgggca tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca
    14401 acgccgctct attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc
    14461 tttatgatca cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa
    14521 cagccagtgt ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc
    14581 ctgaactcgt ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac
    14641 aacggagtga tccagttgac tggtgttgcc agccgctatg tcggttttgt cgtcgcgctg
    14701 atgttgatcg tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa
    14761 ccggttctgg gcggcgcaac gcttgtaatg tttggcacta tcgccgcctc cggtgtgcgt
    14821 atcgtttctc gtgaaccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg
    14881 gttggtctgg gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa
    14941 aacctgctct cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt
    15001 ttcccaccag aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca
    15061 cctctttcac cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg
    15121 catttcacgg gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc
    15181 gctctgttag tggtgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa
    15241 catatcagcg catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggat
    15301 caccgttttt ccgcgccatc tcatatcgtg ctggagaacg tcacgtttgg ccgtgatggc
    15361 cagcccgcga ccctggtggc aaaaagtgtc gacattgcgc taagcagtcg gcaactgacc
    15421 gaaccacgcc atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag
    15481 accgcgccgc taccgttcaa agccgatcgg ctgcaactgc gtgatatggc gtttaatagc
    15541 ccgaatagcg aatggaaact gagcgcgcag cgggtaaatg gcggcgtagt tccgtggtca
    15601 ccaaaagccg gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt
    15661 tcgctcaatg atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc
    15721 gttacgctga ctaacctggg tgccgacatc gcccgcggga cattaaccgg aaacgcgcag
    15781 cgtaacgccg acggcagctg gcaagtggaa aacctgcgca tggcggatat acgtctacaa
    15841 agcgaaaaat cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt
    15901 ggtcgcctgg aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc
    15961 gatctcagct tgcgcaacat gaccttcagt aaagatgact ggcagacaca ggaaggcaaa
    16021 ctgtcgatga acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata
    16081 aacgcggaat tttccccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg
    16141 ggtatggtca gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat
    16201 gtggcaattg ccgggctgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa
    16261 acgacacccg gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg
    16321 atcattgata tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc
    16381 aacctgacgc tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat
    16441 gccgccgccg cgacattcaa tcgtgttgat gttcgtcgcc cgtcgctggc gctgaccgcc
    16501 aacagcagca cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa
    16561 gccactgcca gtgtttcaca aacgccacaa cgtcagaccc atatcagcct gaatggacgc
    16621 ggtgtgccgg tgaatatttt gcaacaatgg ggatggcctg aattaccgtt gactggcgac
    16681 ggcaatattc agcttaccgc cagtggagat attcaggcca atgccccgct gaaacctacg
    16741 gttagcgggc aattgcatgc cgtgaacgcc gcaaagcagc aagtgactca aaccatgaat
    16801 gctggcatcg tttccagcgg tgaagttacg tcggcagagc tggtgcagta aacatcaatc
    16861 tcgccgggta cgactcagac gtacccggca ttccatcaat agattacaac gtaattgtca
    16921 gcgcattccc ttgaggcttc accaccagcc cctgctcact ttcagcctgc gaaccgtctt
    16981 gcagaccatt tactttcaca acattgcgca ggcacagtgt ccagttcttc gcctcgcccg
    17041 caccagtcac agtaatcgtg ttgccagtac gtgctgcttt taaagtaaag atcaccgatc
    17101 cgtcagcagc gggcacttca cagacggctt catgcccgtc ttgcagatta aagaggtgga
    17161 atgccgtgcc ttcgtgccac acgtaatcgg gacgttgatc gttgttgccc agcgccagta
    17221 gagtgttatc acgcacataa acgggcagac tcaggaagcc gtgctgctgt ttatgccagc
    17281 gactaccgtc gagttcatcg ttgtgccaca ggtgtgtcca gcgaccttcc ggcaggtaga
    17341 actgcacatc gcccgcttca gtgaacaccg gcgcaaccat cacgttgtcg cctaacatgt
    17401 attgacggtc aaggtaatca caagccggat cgtccgggaa ctccatcatc atggcccgca
    17461 tcatcggcgt accccgcgcg ttcgcacgcg cagcttcacg atacagatac ggcatcatgc
    17521 ggcatttcag ttgcgtgaag aagcgcacta catcacagga ctcatcatcg taggcccacg
    17581 gcacacgata agatttgcta ccgtgtaaac ggctatggct ggagagcaaa ccaaacgcgc
    17641 accagcgttt gtaaacgtgc gccggagcgg tattttcaaa gccgccgata tcgtggctcc
    17701 agaagccaaa acctgaaagg ccaatagaca aaccaccgcg caggctttcc gccattgatt
    17761 cgtagttagc gtaacaatcg ccaccccagt gtaccgggaa tttctgcgca ccgacggagg
    17821 ccgagcgggc aaacaagaca gcttcttcct caccaacggt gtccttgagc acgttccaca
    17881 ccagttcgtt gtagatgtat gcataatggt tatgcatttt ctgcggatcg gaaccgtcaa
    17941 accactgaac atcagttggg atacgttcgc caaagtcggt cttaaagcaa tcaacgccca
    18001 tcgcgaccag acctttcagt ttgtcggcgt accatttgca ggcatccgga ttggtaaagt
    18061 cataaatcgc cagacctggc tgccatttat cccactgcca tagcgaaccg tccgggcgtt
    18121 tgagtaaata gcctttctct tgtaactctt taaagacggg ggatttttga ccgatatagg
    18181 ggttaatcca gacgcagatt ttcagccctt tcgctttcag gcggcggatc atcccttccg
    18241 ggtcagggaa agtcagcggg tcccactcaa aatcgcacca ctggaaggct ttcatccaga
    18301 aacagtcaaa gtggaaaaca tgcagcggca gattgcgttc cgccatacca tcgataaagc
    18361 tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt ggttagccac aggccgaagg
    18421 accacgcggg cggcagcgcc ggacgaccag taaagcgggt ataacgatca agtaccgctt
    18481 tcggcgtcgg gccgtcgata acaaagtatt cgagatattc actctcaacg ctgaactgca
    18541 ctttggagac tttctccgat cccacttcaa aagagacaca ctggggatga ttgaccagta
    18601 ccccataacc acggttagtc atgtagaacg ggatattttt atacgcctgt tcagtacttg
    18661 tgccgccgtc ccggttccag gtctctaccg tctggccatt gcgcaccagg gcagtaaagc
    18721 gctctcccag accgtaaact gtttcgccaa cgccaagatc aagccgctca aacatataat
    18781 tgcgttgatt attcgtgtcc tgcacgtagc cattattttt cacctgacta ccggtaatac
    18841 gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc tttgctgaca cgcgcgctta
    18901 agttgccact tttaaactca gcataacgtt ctgtgttttc gattgtgacc ttcacgtcct
    18961 gcaaaatatt gagcggataa tgaggaccgt tattcagcgc cccctgaaaa tgctcaatcc
    19021 gcacaccgac aataccttcc tgtggggaga aaaagcgcag cgtaaataaa ggcgtatcaa
    19081 gctgccaggt acgttcacgc acatcacggg gggcagcata gaccaccatt tcattaccct
    19141 gctgttcaac ctcgaatacc tgaagcgggt gaatcaaatt gaggccaggt tgaatcaacc
    19201 agtttccatc gctaattttc attgccgttc cttagttctg cacttcttga gaggtgaatt
    19261 gttgctggca acggcgttta ccctgagcca gctgttccat aacggttttc agattgtgcg
    19321 tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag caaataacag atcgccggaa
    19381 caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct gttctgcgct ttttctgccg
    19441 catcatatcc gccataagcc agcatccagc cgataagagc gccgccgaag gccaacccca
    19501 gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt caggcgttta ccattgcacc
    19561 actcgccgta gtcgacggta tcggacatca ttacccactg gataggtgtc accagttgat
    19621 gcaacacacc aatcacgaag atgaagacaa acatagtgat gctggcctgc atgggaacaa
    19681 agaacatcgc gaggctaatc actgccagca gggcgttcgt ccaccagaag atagtgactt
    19741 tacatttcca gtcggtcaga ggttttgcca gtgcggaacc aatcaggtta cccacgcaat
    19801 aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa aatccatgtg acgtaataca
    19861 tcatcgcccc accgcgtacg cacaccgcca ggatattgaa aatggttagt aaaccgacaa
    19921 tccgccactg gtcgttttgc cagatatcac gtaaatcttc ccgcatagac gttgttgtag
    19981 gtggtgcttc aacgcgttct ttagtggtga agaaacagaa tgccagcatc atgaatgcca
    20041 ccacggaaag gaccgcgata ccgccctgga aaccgagtgg tttattatca ccgccaatta
    20101 aattaaccag tggcatcatc agaacagtag aaagcatgcc tcccgcggtc gccagcacaa
    20161 aacgccagga ttgcagcgag atacgctgag tcgggtcatt ggtgattaca ccacccaatg
    20221 cgcagtaagg gatattgacg acggtataaa gtaaggtaag tagggtgtaa gtaattgctg
    20281 catagatcat tttgccgttc atactgagat ctggcgtgct ataggccagt acacagacga
    20341 tcccgaatgg cagtgcgcca aacagtaccc acggacgaaa tttaccccag cgagagcgcg
    20401 ttcgatcggc caacaacccc atgcaaggat cggaaatcgc atccagtgca cgagcgacca
    20461 aaaacatggt tccgacaaaa ccggcaggaa tgccaaaaat atcggtataa aagaacatca
    20521 tatataacat tacgttatcg aaaataatgt ggctggcggc gtctcccatg ccataaccaa
    20581 ttttctcttt aacggacaac acttcactct tcatctgctt ttccttgatg ctataggggt
    20641 gctgagaccg ataacgcatt ttgtaaacca tccctgtgag agcgaatatt ccgatatctg
    20701 gttattaatt tatgattctt gttttatgtg atcgtggtag cgttaatttc gctcatatat
    20761 cattgtaaaa tataggtttt atatgaacta taatgctttc gtgataatac gctgcgtgta
    20821 ttaggcggaa aaaactgatc tgggggatgt agaaactcaa ggaagtagct ataatgcgcc
    20881 ccgcctccat gtagcaatcg aggcgcggaa gatcgtcgtc tccggtgagg cggctggact
    20941 tcaaatccag ttggggccgc cagcggtccc gggcaggttc gactcctgtg atcttccgcc
    21001 aaaatgcctc ttctgacgtt ctccaacccc ttgtaatact gtcaatccag atattttcta
    21061 ctgctcaata ttcaatcggc ttcaggaata cctggtctaa ggttttgtga tggacgatga
    21121 gcggctgaag aatccgcctg tcggttcttc tgtcctcgaa cgcatccgcg atatgcgcgc
    21181 cagcatgtgc cgtgtttatt tgcaggtgat ttgccccctg ctggctctca gccaactgta
    21241 tcgccattaa ggtgaagatg ttgttttact cgccccagaa cacccttttt tatacgacta
    21301 ttctatttct ttaaccgctg gcgaaaaaaa ctgactata
//
LOCUS       DAZGTR010000075        20950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGTR010000075 DAZGTR010000000
VERSION     DAZGTR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.075"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(215..1864)
                     /gene="actP"
                     /locus_tag="ACHLCY_003094"
     CDS             complement(215..1864)
                     /gene="actP"
                     /locus_tag="ACHLCY_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP0370301.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1861..2175)
                     /gene="yjcH"
                     /locus_tag="ACHLCY_003095"
     CDS             complement(1861..2175)
                     /gene="yjcH"
                     /locus_tag="ACHLCY_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP0370302.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2376..4334)
                     /gene="acs"
                     /locus_tag="ACHLCY_003096"
     CDS             complement(2376..4334)
                     /gene="acs"
                     /locus_tag="ACHLCY_003096"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP0370303.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4726..6162
                     /gene="nrfA"
                     /locus_tag="ACHLCY_003097"
     CDS             4726..6162
                     /gene="nrfA"
                     /locus_tag="ACHLCY_003097"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP0370304.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6207..6773
                     /gene="nrfB"
                     /locus_tag="ACHLCY_003098"
     CDS             6207..6773
                     /gene="nrfB"
                     /locus_tag="ACHLCY_003098"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP0370305.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6770..7441
                     /gene="nrfC"
                     /locus_tag="ACHLCY_003099"
     CDS             6770..7441
                     /gene="nrfC"
                     /locus_tag="ACHLCY_003099"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP0370306.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7438..8394
                     /gene="nrfD"
                     /locus_tag="ACHLCY_003100"
     CDS             7438..8394
                     /gene="nrfD"
                     /locus_tag="ACHLCY_003100"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP0370307.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8414..10132
                     /gene="nrfE"
                     /locus_tag="ACHLCY_003101"
     CDS             8414..10132
                     /gene="nrfE"
                     /locus_tag="ACHLCY_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP0370308.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10125..10508
                     /gene="nrfF"
                     /locus_tag="ACHLCY_003102"
     CDS             10125..10508
                     /gene="nrfF"
                     /locus_tag="ACHLCY_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP0370309.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10505..11101
                     /gene="nrfG"
                     /locus_tag="ACHLCY_003103"
     CDS             10505..11101
                     /gene="nrfG"
                     /locus_tag="ACHLCY_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP0370310.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11443..12732
                     /gene="gltP"
                     /locus_tag="ACHLCY_003104"
     CDS             11443..12732
                     /gene="gltP"
                     /locus_tag="ACHLCY_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP0370311.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLG"
     gene            complement(12834..13523)
                     /gene="yjcO"
                     /locus_tag="ACHLCY_003105"
     CDS             complement(12834..13523)
                     /gene="yjcO"
                     /locus_tag="ACHLCY_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP0370312.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13617..15764)
                     /gene="fdhF"
                     /locus_tag="ACHLCY_003106"
     CDS             complement(13617..15764)
                     /gene="fdhF"
                     /locus_tag="ACHLCY_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15345..15347),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFP0370313.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGLQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15962..17428)
                     /gene="mdtP"
                     /locus_tag="ACHLCY_003107"
     CDS             complement(15962..17428)
                     /gene="mdtP"
                     /locus_tag="ACHLCY_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP0370314.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTSIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17425..19476)
                     /gene="mdtO"
                     /locus_tag="ACHLCY_003108"
     CDS             complement(17425..19476)
                     /gene="mdtO"
                     /locus_tag="ACHLCY_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP0370315.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLFIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDRRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19476..20507)
                     /gene="mdtN"
                     /locus_tag="ACHLCY_003109"
     CDS             complement(19476..20507)
                     /gene="mdtN"
                     /locus_tag="ACHLCY_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP0370316.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVMPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20526..20801)
                     /gene="ytcA"
                     /locus_tag="ACHLCY_003110"
     CDS             complement(20526..20801)
                     /gene="ytcA"
                     /locus_tag="ACHLCY_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP0370317.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4743 a         5534 c         5715 g         4958 t
ORIGIN      
        1 gagagaaaaa catattattt aaaccattat agcgcataaa atatccttat aattaacaag
       61 aaagaaaagg catattctct gcattatcat tttctacgac tgtcaaaaat cgctcatttt
      121 ttaatgagtt tatttgttta atattatggg aaaaggtgat gcatgctgga gaggaagagt
      181 attccccggt caggcgaccg gggaagggga gagattaatg tgcgcggcct tgctcaacgc
      241 caaagccggt ctgggagcgg ataaactgtg cacggaacag ttcacgctcg cgcgcacctt
      301 ccgctgagtt atcggttgcc gagaagaacc agatgccgag gaatgccacg ctgatagaga
      361 acagcgccgg gtattcatac gggaagatgg ctttttcgtg accaaggatc tgtacccaaa
      421 tcgtcgggcc gaggatcatc agtaccactg cggtaatcag ccccagccag ccacccatca
      481 tcgcgccacg agtggtcagt ttcgaccagt acatagaaag cagaatgatc gggaagttac
      541 agctcgctgc gatggcaaac gccaggccta ccatgaaggc gatgttctga ttctcaaaca
      601 gcacgccgag gataatggca atcacaccta aaatcagcac ggtgattttc gatacccgca
      661 gttcttcacg ttcggtcgcg ccttttttga agacgttagc gtacaggtca tgcgaaaccg
      721 ctgatgcgcc cgccagcgtc agaccagcaa ccaccgcaag aatggtggca aaagcaaccg
      781 ctgaaataaa accgaggaac aggttgccgc ccactgcatt cgccaggtga acggccgcca
      841 tgttgttacc accaatcaga tggcccgccg cgtctttata ttccggattc gcaccaacca
      901 gcatgatcgc gccgaagccg ataataaagg tcagaatata gaagtagccc ataaacccgg
      961 tggcgtagaa cacgctctta cgtgcttcgc gggcatcgct gactgtaaag aagcgcatca
     1021 gaatgtgcgg caagcccgcc gtaccaaaca tcagtcccag acccagagag agcgcggaga
     1081 tcggatcttt caccagcccg cccggcttca tgatgtcgac acctttcggg tgtaccgcca
     1141 tcgcttcact gaacagattg ttgaagctaa agccgacgtg tttcatcacc ataaaggcca
     1201 taaagctggc accgaacagc aacagcacgg ctttgataat ctgtacccaa gtggtcgcca
     1261 gcatgccgcc gaacaggacg tacatcatca tcagcacgcc gaccagcacc accgcaatgt
     1321 gatagttaag gccaaacagc agctcgatca gtttacctgc gcccaccatc tgggcgataa
     1381 ggtaaagcgc caccaccacc agagaaccac aggccgaaag aatacgaatc ggcccttgtt
     1441 tcagacggta agaggccaca tcggcaaagg tgtagcgccc caggttacgc agacgttcgg
     1501 caatcaggaa caaaatgatc ggccagccca ccaggaagcc cagcgagtaa attaagccgt
     1561 catagccgga ggtaaacacc agcgcggaga tccccaagaa tgaggcggcg gacatatagt
     1621 ccccggcaat cgccagcccg ttctggaagc cagtgatatt gccgcctgcg gtgtagtagt
     1681 cgctacgaga acgtacgcgt tttgatgccc agtaggtaat gccgagcgta aacacgacga
     1741 aaatcaggaa cataataatc gcctgccagt tcgttggctg gcgctctacg gccccgctaa
     1801 tagcatccgc ggcgttagct gcgaaaggga gtgtggcggc aagcgccgtc agaactcttt
     1861 tcatgatgct tgtacctcat gcaggacttc attattaaga cggtcgaatt cgccgttcgc
     1921 ccgccagatg tagataccgg tgagaacaaa ggagatcaca atcactccaa caccaattgg
     1981 aataccgcgt gtgacgctgg tgttcggatt cagcggcgtg cccagccagc cgggcgcgaa
     2041 ggcgatcagt aaaataaagc cgatataaac tgccagcata ataatcgaca ggatggtggc
     2101 aaaccgttgc cgtttttcga ctaactccct gaaatgcgca ttgtcttcta tccgctgata
     2161 aatcgtacca ttcatcacag attctccaga ggtaatgtag ggattgtttt aattcccgct
     2221 cccttatggg agaaggttaa cgctcgggta acccttgccg aatgtaggcc ggataaggcg
     2281 ttatacgccg catccggcaa tcaatgcctg atgcgacgct gtcgcgtctt atcaggccta
     2341 caaaccgtta ccgactcgca tccggcaatt gtgggttacg atggcatcgc gatagcctgc
     2401 ttctcttcaa gcagcttctc gactacgcca ggatcggcaa gcgtcgaggt atcgcccagg
     2461 ttgctggtat cgcccgccgc aattttgcgc agaatacggc gcataatttt gccggagcgg
     2521 gttttaggca gggagtcggt ccagtgcagc acgtctggcg tcgccagcgg gccaatctct
     2581 ttacgcaccc agttgcggac ttctgcgtac agttctggtg acggttcctc cccgtgatta
     2641 agcgtgacgt aggcgtagat cgcctgacct ttaatattgt gcggaatacc tactacggcg
     2701 gcttcggcaa tcttcggatg cgccaccagc gccgactcaa tctctgccgt ccccagacgg
     2761 tgaccggaga cgttcagcac gtcgtccaca cgcccggtga tccagtaata gccatcttca
     2821 tcgcgacgcg cgccgtcgcc gctgaaatac atatttttga aggtggagaa gtaggtctgt
     2881 tcaaaacgtt cgtgatcgcc aaacagcgta cgcgcctgac ccggccagga gtcggtgatc
     2941 accaggctgc cttcggtagc gccttccagc gggttacctt cgttatcgac cagcgccggt
     3001 tgcacgccga agaacggacg tgttgccgaa ccggctttca gctcggtagc gccaggcagc
     3061 ggcgtgatca tgaaaccgcc agtttcggtc tgccaccagg tatcgaccac cggacatttc
     3121 tcgttgccga tttttttcca gtaccactcc cacgcttccg ggttaattgg ctcgcccacg
     3181 gaaccgagaa tgcgcagcga cgaacggtcg gtaccctcga tcgctttatc gccttccgcc
     3241 atcagcgcgc ggatcgccgt gggtgcggta tagagaatat tgacctgatg cttgtccacc
     3301 acctgcgcca tacgggcagg cgtcggccag ttgggtacgc cttcaaacat cagcgtggtc
     3361 gcaccgcagg ccagcgggcc gtacagcaag taactgtgtc cggtcaccca gcccacatcg
     3421 gcggtgcacc agtagatatc acccggatga taatcaaaga catatttaaa ggtcagcgcc
     3481 gcgtacacca gataaccgcc ggtagtatgc agcacacctt ttggcttacc ggtagaaccg
     3541 gaggtgtaga gaataaacag cggatcttcg gcgttcatct cttccgcctg gtgctgatcg
     3601 ctcgcttgct caaccaggtc gtgccaccac aggtcgcgcc cttcctgcca gtcaattttc
     3661 ccgccagtac gcttcagtac caccacatgc tctacgctgg tgacgttcgg gtttttcagc
     3721 gcgtcatcaa cgtttttctt cagcggaata ctgcgcccgg cacgcacacc ttcgtcggaa
     3781 gtgatcacca gtcgtgagtt ggaatcaata atgcgcccgg caacggcttc cggcgagaag
     3841 ccgccgaaaa tcaccgaatg caccgcgcca atgcgggcgc aggccagcat cgcaaccgcg
     3901 gcttccggca ccatcggcat ataaatcgcc accacatcac cttttttaat gcccagctcg
     3961 agcagggtat tggcgaagcg gcacacgtcg cggtgcagct ctttatagct gatatgtttg
     4021 ctctggctgg cgtcgtcgcc ttcccagatg atggcggtgc gatcgccgtt ttcttgcaga
     4081 tggcggtcaa ggcagtttgc cgccagattc agcgtgccgt cctcgtacca tttaatggac
     4141 acattaccgg gggcaaagga ggtatttttc accttctggt acggtttgat ccagtcgaga
     4201 atttttccct gttcgcccca gaaggtatca ggtacgttaa tagattgttg atacatcgcc
     4261 tcgtactgct gagggtttat caggcaacgg tctgcgatgt tggcaggaat ggtgtgtttg
     4321 tgaatttggc tcatgctttt gttctccttg taggatgtta ataatatgtg gcataagcgt
     4381 taaatgtagg ggtattggca gttttgttta gtatttgggc gacagatcac gcaaaagtag
     4441 aattgtgcaa ataaacggca gggtaatttt tgaaggtcac caacaaaagt tgattaattc
     4501 tttgaggaac atgcagttat gcatgctgtt gaaaaagagg aagatactga ctaactctaa
     4561 agtggtattt tacatgcact tacaattgat taaagacaac attcacagta tggttatatg
     4621 ttatacatag gggcgagcaa tgtcatgaca gtgtaggtgc ggttactgtc gtgaaaacaa
     4681 taaaaaccgc cattgcaaca atggcgcaat tcggatgaag cccctatgac aaggataaaa
     4741 ataaacgcac gccgtatctt cagcttattg attccttttt tctttttcac ttctgttcac
     4801 gctgaacaaa cggcagctcc cgcaaaacct gtaactgtgg aagcgaagaa tgaaaccttt
     4861 gccccgcagc atcccgatca atatctctcc tggaaagcca cctcggaaca gtcagagcgt
     4921 gttgacgccc tggcggaaga tccacggctg gtgatcctgt gggcggggta tcccttctcg
     4981 cgcgattaca acaagccgcg tggacatgcc tttgctgtga ccgatgtgcg tgaaaccctg
     5041 cgtaccggtg cgccgaaaaa cgctgaagat ggtccgctac cgatggcgtg ctggagttgt
     5101 aaaagcccgg atgtggcgcg tctgatccag aaagacggcg aagatggcta cttccacggt
     5161 aagtgggcgc gcggcggccc ggaaatcgtc aacaacttag gttgtgccga ctgccataac
     5221 accgcctcac cagagttcgc caaaggtaaa ccggagttaa ccctttcccg tccgtatgcg
     5281 gctcgcgcga tggaagccat tggtaaacct tttgagaaag ccggacgttt cgaccagcaa
     5341 tcgatggttt gcggtcagtg ccatgtggag tattacttcg acggcaaaaa caaagcggtt
     5401 aaattcccgt gggatgacgg catgaaagtc gaaaatatgg agcagtatta cgacaaaatt
     5461 gccttctctg actggactaa ctccctgtcg aaaacgccaa tgctgaaagc gcagcaccct
     5521 gaatatgaaa cctggacagc gggcattcac ggtaaaaaca acgtgacctg tatcgactgc
     5581 catatgccaa aagtgcagaa cgccgaaggc aaactctaca ccgaccataa aattggtaat
     5641 ccgtttgata acttcgccca gacttgtgcg aactgccata cccaggacaa agctgctttg
     5701 caaaaagtgg tcgcggaacg taagcagtcg attaacgacc tgaaaatcaa ggttgaagat
     5761 caactggttc acgctcactt cgaagcgaaa gcagcgctgg atgcaggcgc gacggaagcc
     5821 gaaatgaagc cgattcagga cgatatccgt catgcccagt ggcgttggga tctggcgatc
     5881 gcttcccacg gtattcatat gcacgcaccg gaagaaggtc tacggatgct cggtacggcg
     5941 atggataaag cggcggatgc acgcaccaaa ctggcgcgcc tgctggcgac caaaggcatc
     6001 acccatgaaa tccagatccc ggatatctcg accaaagaga aagcccagca ggccattggc
     6061 ctgaacatgg aacaaatcaa ggccgagaag caggacttca tcaaaacggt gatcccgcag
     6121 tgggaagaac aggcacgtaa aaatggtctg ttaagccaat aaccccgttc cgcctcgcaa
     6181 ggggcggaaa acacaatgga gtgaatatga gcgtattacg ttcgttatta actgccgggg
     6241 tgctggcgtc gggcttgttg tggagcctga acggaattac cgctacccct gcggcgcagg
     6301 catctgatga tcgctacgaa gttacccagc agcgtaaccc ggatgccgcc tgtctggact
     6361 gtcataaacc agataccgaa ggtatgcatg gaaaacatgc ttccgttatc aacccgaata
     6421 acaaactgcc ggtcacctgc accaactgtc acggccagcc atcaccgcaa caccgcgaag
     6481 gggtgaaaga tgtgatgcgc tttaacgagc cgatgtacaa ggttggagag cagaacagcg
     6541 tctgtatgtc ctgtcatctg ccggaacagt tgcaaaaagc gttctggccg cacgatgtcc
     6601 acgtaaccaa agtggcgtgc gccagctgcc attccctgca tccacagcaa gatacgatgc
     6661 agacgttaag tgacaaagga cggattaaga tttgcgtcga ttgccacagc gatcagcgca
     6721 ccaatccgaa ctttaacccg gcgtctgttc cgttgcttaa ggagcagcca tgacctggtc
     6781 tcgtcgccag tttctcaccg gcgtcggcgt gctggcagcc gtcagcggca ccgcagggcg
     6841 cgtggtggcg aaaacgttga atatcaatgg ggtgcgttac ggcatggtgc atgacgagtc
     6901 gttatgcatc ggctgtaccg cctgtatgga tgcttgtcgg gaagtgaaca aggtgccgga
     6961 aggcgtctcg cgcctgacga ttattcgtag cgagccgcag ggcgaatttc ctgatgtgaa
     7021 atatcgcttc ttccgtaagt cttgccagca ctgcgatcat gcgccgtgcg ttgacgtctg
     7081 cccgaccggt gcgtcttttc gtgatgccgc tagcgggatt gtcgatgtta atcctgatct
     7141 ctgcgtcggt tgtcagtact gcatcgccgc ctgtccgtac cgcgtgcgct ttatccatcc
     7201 ggtcacgaag acggcggaca aatgcgattt ctgccgtaaa accaatttgc aggccggtaa
     7261 gctgcccgcg tgcgttgagg cttgcccgac gaaggcgctg acgtttggca atctggacga
     7321 tcctaacagt gagatttcgc aactgctgcg ccagaagcct acttaccgct acaagctggc
     7381 gctgggaacc aaaccgaagc tgtaccgcgt accgtttaaa tacggggagg tgagccaatg
     7441 acgcagactt ccgcatttca ttttgaatcg ctggtgtggg actggccgat tgccatctac
     7501 ctgtttttga ttggtatttc tgccggtctg gtgacgctgg ccgtgctgtt acgtcgcttc
     7561 tacccgcagg cgggcggtgc agacagtacg ttgctgcgca ccacgctgat tgtcgggccg
     7621 ggcgcggtga tcctcggtct gttgatcctc gtcttccacc tgacaagacc gtggaccttc
     7681 tggaagctga tgttccacta cagttttacc tcggtgatgt cgatgggggt gatgctgttt
     7741 cagctctaca tggtggtgct ggtgctgtgg ctggcgaaaa tctttgaaca tgatttgctt
     7801 gccctgcaac aacgctggtt gccgaagctg gggatcgtgc aaaaggttct gagcctgctg
     7861 acgcccgttc atcgcggact ggaaacattg atgctggtgt tggcggtgtt gttgggggct
     7921 tataccggct ttctgctgtc ggcgctgaaa tcgtatccgt tcctcaataa cccgatcctg
     7981 ccggtgctgt tcctcttctc cggcatctcg tccggtgcgg cggtggcgct gatcgccatg
     8041 gcgatacgcc aacgcagtaa cccgcattcc acggaagcgc agtttgtaca ccgtatggaa
     8101 atccccgtgg tatggggtga aatcttcctg ctggtggcat tttttgtcgg tctggcgctg
     8161 ggcgatgacg gtaaagtgcg tgcgctggtg gcggcattag gtggcggttt ctggacgtgg
     8221 tggttctggc ttggtgtcgc cgggctgggg ctgattgtgc caatgttgct caaaccgtgg
     8281 gtcaatcgca gttccggcat tcctgccgtg ctggcggcgt gtggggccag tctggtcggc
     8341 gtgttgatgc tgcgcttttt cattctctac gccggacagt tgacggtggc gtaagccaga
     8401 aaagaggtgg tttctggacg tattccttcc tgaagtcggt tttctggcgt tgttgttaag
     8461 tctcggggtc aacgtgttga ccccgttgac ggcctttgcg ggagtgcggt tgcgctggcc
     8521 tgccatgatg cgactcactt gcatcggcat tctggcgcag ttcgcgctcc tgctgctcgc
     8581 ctttggcgta ctgacgtatt gttttctcat cagcgatttc tcggtcattt atgtcgcgca
     8641 acatagctat agcctgctgt cgtgggaact caagctggca gcggtgtggg gcggccatga
     8701 aggttcgctg ctgctttggg tgctgctgct ttccgcctgg agcgcgctgt ttgcctggca
     8761 ttatcggcag caaaccgatc cgctatttcc gctgacgtta gccgttttat ctctcatgct
     8821 cgccgcactg ctactgtttg tggtgctgtg gtccgatccc ttcgtgcgga tatttccacc
     8881 agcaatcgaa ggccgcgatc tcaatccgat gctgcaacat cccggtctta tctttcatcc
     8941 accgctgctt taccttggct atggcggttt gatggtagcg gcgagcgtgg cgctggcgag
     9001 tttactgcgc ggcgagtttg atggtgcctg cgccagaatt tgttggcgct gggcactacc
     9061 tggctggagc gcattaacgg cggggatcat cctcggttcc tggtgggcct attgcgaact
     9121 gggctggggc ggctggtggt tctgggatcc ggtggaaaac gcctctttat taccctggct
     9181 ttctgccact gcgctgctgc acagtttgtc cctgacacgc cagcgggaga ttttccgcca
     9241 ctggtcgctg ttgctggcga tagtaactct gatgctgtcg ctactgggta ccttaattgt
     9301 ccgttctggc attctggttt cggttcatgc gttcgcactg gataacgtcc gcgccgtgcc
     9361 gttgttcagc ctgtttgcac tgattagcct tgcgtctctg gctctgtatg gctggcgagc
     9421 gcgggacggt ggtgcggtgg tgcgtttttc ggggttatcg cgggaaatgt taatcctcgc
     9481 tacgctgttg ctgttttgcg cagtgctact gatcgtgctg gtgggaacgc tttatccgat
     9541 gatttacggc ctgctgggct ggggacgcct ctccgttggc gcgccgtatt ttaaccgcgc
     9601 gacgttaccg tttggcctgt tgatgctggt ggtgattgtg ctggcgacgt ttgtctctgg
     9661 caaacgcgtg cagcttccgg cgctggtagc tcatgcgggc gtgctgttat ttgccgcggg
     9721 gatcgtggtt tccagcgtca gccgtcagga gatcagcctg aatttacagc cgggtcagca
     9781 ggtgacgctg gcaggataca ccttccgttt tgagcgcctc gatctgcaag ccaaaggcaa
     9841 ttacaccagc gaaaaagcga tagtggcact gtttgaccat cagcaacgca ttggtgaact
     9901 gatgccggag cggcgttttt acgaagcacg tcgtcagcaa atgatggaac cgtcaattcg
     9961 ctggaacggc atccatgact ggtatgcggt catgggtgaa aaaaccggag cggatcgtta
    10021 cgcttttcgc ttgtatgtac aaagcggtgt gcgctggatc tgggggggag gattgttgat
    10081 gattgcgggc gcattgttaa gcggatggcg ggggaggaag cgcgatgaat aaaggacttc
    10141 tcacgctact gctgttattt acctgttttg cgcgcgctca ggtcgtagac acctggcaat
    10201 tcgccaatcc gcaacaacag caacaggcgt taaatattgc cagccagtta cgttgtccgc
    10261 agtgccagaa tcaaaactta ctggaatcca acgcgccggt ggcagtcagt atgcgccatc
    10321 aggtttacag catggtggcg gaggggaaga acgaagtcga aatcattggc tggatgaccg
    10381 aacgctacgg agattttgtt cgctataacc caccgttaac gggtcagacg ctggtgttgt
    10441 gggcgctgcc agtggtgttg ttactgctga tggcactgat cctctggcga gtgagggcga
    10501 agcgatgaaa gagcccaaaa taccggtgaa aatgctgaca acactcacga ttttgatggt
    10561 atttctctgt gtcggcagtt atctgttaag tccaaaatgg caggcggtac atgcggaata
    10621 tcagcgtcag cgcgatccgc tacatcagtt tgccagccag caaaccccgg aagcgcagct
    10681 tcaggcattg caggataaaa tccgcgctaa tccacaaaac agcgaacaat gggcgttact
    10741 gggcgagtac tatctgtggc aaaacgatta cagcaattcg ctgctggcgt accgtcaggc
    10801 gttgcaacta cgtggtgaga acgctgaact gtatgcggcg ctggcgacgg tgctttatta
    10861 ccaggccagc cagcatatga ccgcccagac tcgcgcaatg atcgacaaag ccctcgcgct
    10921 ggactctaac gaaatcaccg ctctgatgct gctggcttcc gatgcgttta tgcaggcgaa
    10981 ttacgcgcaa gccatcgaac tatggcaaaa agtgatggat ctcaactcac cccggatcaa
    11041 ccggacacag ctggtcgagt cgattaatat ggcgaaattg ttgcagcgga gatcagatta
    11101 atcatctggt gatgaagatt atgttattga gttgtataaa aaataatcga atgaacatat
    11161 gccaaaaata atcaccaatc agtattattg cagattaaac aaataaaaat ctttccataa
    11221 caaatggtta ttcattaatc ctgccataaa ctcgcgttat tatgcattaa tgcagcgaaa
    11281 agctctgttg ttaaagggtt gcgcaacata ccgcgcaaat gatactgatc ataagcgtta
    11341 aaaaaatcta caaaccaacg caacacaatt catgccctgg cagtatgtca cgttctcgcg
    11401 tttctgaacg gggaacggcg ctccattgag gaagttattc tgatgaaaag tataaaattc
    11461 agcctggcct ggcagattct gtttgctatg gtgctgggca ttctcctggg aagctacctg
    11521 cactaccata gcgacagccg cgactggctg gtcgtcaatt tgctctctcc ggcgggtgat
    11581 atcttcatcc atctgatcaa aatgattgtt gtgccgattg tgatctccac gctggtggtg
    11641 ggtatcgcgg gtgttggtga tgccaaacag ctcgggcgta ttggcgcgaa aaccattatt
    11701 tacttcgagg tgatcaccac cgtcgccatc attttgggga tcactctggc gaacgtcttc
    11761 cagcccggtg ccggggtgga tatgtcgcag ctggcgaccg tcgatatctc gaaatatcag
    11821 agcactacgg aagcggtaca aagcagttcc cacggcatta tgggcacgat tttgtcgctg
    11881 gtgccgacga acattgtggc gtcgatggcg aaaggcgaaa tgctgccgat catcttcttc
    11941 tcggtgctgt ttggtctggg gctttcctcc ctgcccgcga cgcatcgtga accgctggtg
    12001 accgtgttcc gctccatctc tgaaaccatg tttaaagtga ctcacatggt gatgcgttat
    12061 gcaccggtgg gtgtgtttgc gctgattgcg gtgacggtgg ctaactttgg tttctcttct
    12121 ctgtggccac tggcgaaact ggtgctgctg gtgcatttcg ccattctgtt cttcgcgctg
    12181 gtagtgctgg gaattgtggc gcgcctgtgc gggttaagcg tctggatcct gattcgtatt
    12241 ctgaaagatg agctgattct ggcgtactcc actgccagct ctgaaagcgt actgccgcga
    12301 attattgaga agatggaagc ctacggagca ccggcgtcga tcaccagctt cgtggtgccg
    12361 accggttact cttttaacct tgatggttcg acgctgtatc aaagtattgc cgctattttc
    12421 atcgctcagc tgtatggcat tgacctgtcc atctggcagg aaatcattct ggtgctgacg
    12481 ctgatggtga cctcgaaagg gattgctggc gtgccgggcg tgtcgtttgt ggtgctgctg
    12541 gcaacgctgg gtagcgtagg tattccgctg gaaggcctgg cgtttattgc tggtgttgac
    12601 cgtatcctcg acatggcgcg tactgcgctg aacgtggtgg gtaatgcgct ggcggtgctg
    12661 gtgattgcca agtgggaaca caaatttgac cgcaaaaagg cgctggctta tgagcgtgaa
    12721 gtgctgggct aatttgataa aactgcggat caataattga agattgccgg ggttatccac
    12781 ccggcaatgt gtggatgcct gatgcgacgc tggtgcgtct tatcaggcct accttaaccg
    12841 ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc
    12901 aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcctcacc gtttaagaac
    12961 atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa
    13021 taccaggttg ccttgtcatc gtcggcctta atgcccacgc cgttggcgta aatcaaacca
    13081 agcagcattt gggcatcgac ggcggagtca ttctccagat cttccgaggc gttttctaac
    13141 agcgaaatcg cctttggata atccggttta cccgcttgag tatttaccag aatatgcgcc
    13201 agcgttactt caccttcttt actgcccgct tgcgccgctt tctctgcaag aacttttgct
    13261 tgtggatagt ccagactgac cggattggtg attttgatct gcgccagcag cgcgcaggca
    13321 tcagcatcac cgctgtcggc ggctttctgt gcccaatatt cggctttgct caaatcgccg
    13381 gagctaaacc agctgtcggc aagaaaatat tgtgcgcgtc ggtccccggc ctctgctgcc
    13441 tttaaatact ggctgccagg ctcggagtcg ttcgcgtggg caaagaatgt caaaaacaac
    13501 atcaatgcga taattttttt cattttgata atacgattgg gtagggagta accagtataa
    13561 agagttcgaa tggataaaaa aacagcctcc gaaaggaggc tgtagaaagg actgtattac
    13621 gccagtgccg cttcgcgcag gcgagttttc aacttgttgt actcgtcaat cacgtactgc
    13681 tcggcggcgc gctgatcggc gatcggctcg acgcgcacgg cgcagtattt gtattccggc
    13741 gttttcgtaa tcgggcttaa gttttcggta accagctcgt tacaggcacc aatccaccac
    13801 tggtaggtca tgtaaatcgc ccctttgttc ggacgatcgc tgacctgcgc acgggtgata
    13861 attttgcctt tacgcgagtg cacccaaacc aatgcctcat cttcaatacc cagacgtttg
    13921 gcgtcttcgg tattgatttg tgcgtagcca ggttcatcag ccagcgccgc cagtgccgca
    13981 cagttaccgg tcatcgaacg gcaagagtag tgaccaactt cacgcaccgt tgacagtacc
    14041 atcgggtact cgtcggtgag tttgtcgatt ggcgctaccc agtcgcaggt gaagaactgt
    14101 gccagaccgt tcggggtatc aaacttctct ttaaacagat aagaagtccc ctgatcggca
    14161 tctgaagtat cgcggcaagg ccactgaatg aagcccagtt cgcccatttt ctcgtaagtc
    14221 gcaccgtaga aatccgggca cagatgacgc aactcatccc agatctcctg ggtgttgttg
    14281 tagtgcatcg gataacccat acgggtggcg atttcactga tgatttgcca gtccgttttc
    14341 agatcccatt tcggttcaac tgctttgaag aaacgctgga ggccacggtc agccgcagta
    14401 aacacgcctt catgctcgcc ccacgacgtt gacggcaaaa tgacatccgc cgccgacgcg
    14461 gttttggtca taaagatgtc ctgaacgata accagttcca gatcttcaaa ggctttacgt
    14521 actgccgaca gctccgcgtc agtttgcagc ggatcttcgc ccataatgta cgcggcacgc
    14581 acttcgccat gcgctgcgcg gtgcggcagc tcgctgatgc gataaccggt atgagcaggc
    14641 aggctttcca cgccccaggc tttggcgaat ttctcgcggt tagccggatc tttcacgtac
    14701 tggtatcccg gatacgtatc cggcagcgcg cccatatcgc aggcaccctg cacgttgttc
    14761 tgaccacgaa ccgggtttac acccgcatgc ggcttaccga ggttaccggt cagcatcgcg
    14821 aggctggtca gagaacgcac ggtttccacg ccctggtaga actgggttac acccatgccc
    14881 cacaggatgg cggcgctttt cgcctgggca tacatccgtg ccgcctgacg aatctcactg
    14941 gcgctgacgc cggtgatatc ttcaaccgac tccggcgtgt agccttcaac gattttacga
    15001 tactcttcaa agccttctgt acgggaagcg acgaacgctt tgtcgtacag attttcttca
    15061 ataatgacat ggcccatcgc attcaacagc gcgatgttcg agccgttttt cagtgcaatg
    15121 tgcatgtcag caatgcgcgc ggtttcaatt ttgcgcggat cgcagacgat aattttcgcc
    15181 ccgttacgtt tagcgttaat tacgtgattc gccacgattg ggtgggaatc cgccgggttg
    15241 tacccgaaaa cgaacactaa atcggtatta tcaatttcgt taatagcatt gctcattgcg
    15301 ccattaccga ccgattggtg cagacctgca accgatgggc cgtgtcagac acgagcgcag
    15361 cagtcaacgt tattggtacc aataacggcg cgcgcaaatt tttgcattac atagttggtt
    15421 tcgttacccg taccacgcga ggagccggtc gtctggatgg catccggacc gtacttctct
    15481 ttgatggcgc tcaggcgctc ggcaacgtaa ttcagtgcct catcccagga aacaggttcg
    15541 agtttgccgc cacgctggcg acggatcatg ggggttttca ggcgcggggt caggatctgg
    15601 gtatcgttaa tgaaatccca gccataataa cccttcagac acagggtacc ctggttggtt
    15661 ttcccctgcg ctgcctccgc ccggacgatt ttgccgttat cgacgaccag gttgattttg
    15721 caacctgatg cgcaataggg gcaaaccgtg acgacttttt tcatcggtct cgctccagtt
    15781 aatcaaatca cgcatacgcg ctctcgacta cagtatgcat cttttatgcc acattttatt
    15841 tggggtcatt ccctgatatt acgggcacta tttattcaaa actctgacga aaaacaggct
    15901 gtcgtcagtt ttgacgtgac gaaacgaaat accgcgtgac agccatcacg cggcagacat
    15961 tttatttttt ctcgacgacg ggagccgcct gatacccgcc gcccagcgat ttcatcaact
    16021 gaatgctttg gatcacccgg cggctgtcca gcatcagtaa tgacatctct tcggcaagta
    16081 ctggcaaccg ggcttcagtg gcctgtaagc ggctggttaa gccgcgctga taggcggcct
    16141 cggcagcgcg ctgggtgaag cgcgttgctt ccacgcgttc agcctgcatt tctcgctcgt
    16201 cgttgagcgt ttgcagacgc gtgccgttga cggcaacgtc acgcaccgcg ttcagtactg
    16261 actggttgta acgttcaatc atcatgttgc tggcggcgcg cgtgccttcg agattggcat
    16321 tcaaccgtcc accgtcaaac agcggcaatt tcagacccgg gatgaagttg aactggcgac
    16381 tggttttttt gaataaggta tccaggtgga tggcatcaag gccgaaaaac gctttgatat
    16441 caaagctcgg gtagaacagc gcccgcgcgg aatccacctg atctaatgac gcctgaacat
    16501 accagcgcat ggcttgcaga tccgggcgtc tggcgagcaa ctcataagag agtgtcgccg
    16561 gaatgctggt ctggactcgt ggtaatgcca ccggtttgat ctccggcata tcgctggccc
    16621 ccgcgccaat caatgcacgc agagattctc gcgtttcggt gatttgccct ttgacggcag
    16681 caatttgttt atcgaccgcc agaatctgtg cccgcgcgcc gtggaaaggc acttgtgctt
    16741 ccagaccgtg cgccacttta ctctggtgcg ctttcaccgc gtaatcaatc acatcgcgag
    16801 tttgttctaa cagatcgagc atctgatagc tggcctgcat actgtaataa agctgcgcta
    16861 cgcccgtggt cagcgatagc tctactgctg cggtttctgc cagcgcggca ttatgcgcgc
    16921 caatggcggc ggcaaccgct gagcgatgca caccccacaa atcaagatcc agtccggcaa
    16981 acaaacctac tgtggcttcc gtatagtacg gcccgtccat acccagtgcg ggcgcatcca
    17041 tcgcataagg gcttaaaaag ccgttcgccg agacgcgttg gcggttgagc atccctaacg
    17101 ctgcgacctg taattgtgaa ccggcatcta acaaatcggc ctgggactgc gctttttctt
    17161 cccgcagttt cgcttcggcg agggtgtgtg aaccacttag cgtccgttgg atcagcgcat
    17221 ccagctgcgg gtcattgagt tgtttccacc actgcgcctg cggccagccg gagctggcaa
    17281 gatgaatatc gtcggccagt ttgatttgtt ccggtttgag ctgttgatga ggtgcagaat
    17341 ccttacgtac cagggcacag ccggaaatca gcgtcgtgct gccgagaatg ctgcacaaca
    17401 gcagacgtga aagttgacga ttgatcattg cgtggctcct tgtgcctgtt ccgtggcggg
    17461 cgttaatgcc ggggctgtcc agtctggcaa gcgggcgaaa agctggcaga cgtgttgctc
    17521 ctgttttaat aaggtgggca ggatggcctg cgaggcgggt gtctcttcaa gcgttatttg
    17581 aggagaacgg ctgagtgcgg ttgccagacc ggcagcgtat ttctccaggg cgtcggcaaa
    17641 ctgagctgct ctgtccggct gtagtgcgtt atccagcgcc tgcgccgagt tccaggtggc
    17701 atcccaggcg tgaagaatat cgcggccctg acgaataacc gtttgcgaac gttcaatcag
    17761 taaggcgcgt tcttcgctgt ccagttgacg ctccagcgcc acgcgttggc acatctcttc
    17821 acaggcatta aacgccgcat gcagaccgat acgaatttgc agataagtgc gcagagccgt
    17881 gacttcctgc tggcgtggga tccgcattac tttacttaac atacccagcg tgccagccag
    17941 tttttgcggc agtgtgcgcg cttcactttc aggccagaca aaggtgtaaa tcaccgcgga
    18001 caccacggta ccaatgatga tacccagggc gcgatcgcga atttccacca ggtcgtacac
    18061 tgggccaaaa acgttttcga gcgtggcgag cgcgaaggtg accaccatct gtgtgccgat
    18121 ataagaagag cgttcagagc tggtggcaat ccatgcgccc aacaggaaaa tcggtgccag
    18181 cacaaacagc aattcgacaa tattgtccag ccagggcatg accagtagcg tgaataacag
    18241 cgccagaatc gcgccgcaaa aggccccgcc aaaacgcagc accatcttct ggtacgacga
    18301 accgacattt gggttagcga cgatcacgca tgtcagcata caggtgtgaa tgccttccca
    18361 gtccacgccg ctgtaaaagg tgtaacagat caaacaggcg agcagcgttt ttaccgcgta
    18421 gcgcatatag tctggattgg taaaagcatc ggcggccatt gatggcggtt tggctgcggg
    18481 cgttggcggc gtattcgggt ccatctgacc cagttgtaac aacgtctggc agatattctc
    18541 caggttacat tcccgtgccg ccatcgcttc actttcactg atccgcctgt cgctttgcca
    18601 gcactgacct tcagcaacgg catgctgcag cttattgatt tctgaggcta atttttgccg
    18661 aaattcaata attgcctgag aatcagcaaa agaggtggga tcgtagcgat tcagcgtcga
    18721 gtaaatgtag gttaccgttg ccacgcagct ttgccaccat gcgctttgag ttcgccagtt
    18781 ggcatcgtcc gcgagacaaa agacattcag tttttgcagc gccagcgcct ctctttcaat
    18841 ccgcgtttcg ggtagcggtg cgaggctgtc cgtcaggtgg ctaatggcat catcaagccg
    18901 atcattaagc gcctgatgca tttgcgaaat ggcacgactg ggaaaccaca gcacgccgat
    18961 taacgtcatc agcaaggttg gataaaggcc aacaacgata caccacagcg ttaagcgcac
    19021 gaccacttcc ggatagtcga gcatggcggg gaaggtttgc ccgtaaatgg cgacaatggc
    19081 gactgcgaaa aagaccagtc ccagccgatg ggtgcgcatc aaaaacatac agcccatcag
    19141 gatcggcccg gcgatgaaca atcggatcaa cggttcgccg tatgaccatt tgtagatcaa
    19201 aaacaggctg ccgatctcca gcatcgtggc gaccacaaat aagatcgcga caaatttggt
    19261 gtaaaacgcg ttcgactgaa taccgtaaaa cagcactgcc agcgataacg ccacgaaagg
    19321 gatctcaaag gtcatcgaga tcagaatcac cagcaggcag cctacccaga gttgcacggt
    19381 ctgcggcacg cggcctggtc gccgctcgct taactcttca tgaaagaacg ccagcagcct
    19441 gaccaccggt aatggcaggg agttgagcgc gctcattatt gcggctcaag attagcgact
    19501 gccgaagcgc cgatgcggaa catttccggg tcaggtttat cgaccatgat tttgaccgga
    19561 aaacgctggg caacgcggac ccagttaata gagcgagaaa ctttcggcag gccgcccagc
    19621 accaggccgc cgtcatccgg tagcacgccg tagccaatcg aatccacttt accctcgaag
    19681 gttttgccgc tgtcactcat caggcgaatc gttgcgggtg tacctgagcg aatatttttc
    19741 agatcggttt cgcggaagtt ggcgatcacg taccagtgac gagtgtcgat tagggtaaaa
    19801 atagggcgca tggcagaagc aaattgcccg acggaggttt tgagggaaat gacccggcca
    19861 tcaaacggcg cgcgaacggt cgccatttcc agatgcagtt tggtcagggc aatatccgct
    19921 tcgaccgccg cacgctgggc aactaatgca tccacgccgc tgacggcgct ggcggctgac
    19981 tgcgcctgca acaatacagc attaagatcg gcttctgcag cgcgctgcgc ggtcctcgca
    20041 cggtcaacgt cttccgctga gacaaaacct tctttcagta atggctcggt gcggcgtaat
    20101 gtatctgtgg cctgtttcgc ggcggcacgg gctttttcta ccgtggcgtt aaccgagtcg
    20161 gcaccaaact gttgcgcgtc aacgctacgc tgggtgagca taatttgctt atccagcgcc
    20221 gcgagggagg cttcagcttt cgccagattg gcttcgtacg ggcgcgggtc aatgcggaac
    20281 agcaaatcgc cctgtttgac tgcctggttg tcggtgaccg ccagttctac aatgcgaccg
    20341 ctgacttccg gcatcacatc aatggtatct gctgacgcgt aagcgtcatt agttgatggc
    20401 gcactgtcta cgcgccagat aacgaaaaca agggcaacca gcgccaacgc aaccactaac
    20461 agagcaggga atttactgcg aggagctttt ttcggcgtac tttccatgag cgcgtctcaa
    20521 atagattaga aaaatgccag ccagaacagc atggcgtaga gagcaaaaag ggcggtataa
    20581 ataattccga caaatgccag attgatattt gcccgctgaa taactcgcct cgtgatgagc
    20641 atcaaaataa ggctggcaat ggcgcagaaa aaccagctgg gataataagc accgatcacc
    20701 gggattgccg gagataatga gcatcccgaa accatgaaga cgggaattat ccaggctgtt
    20761 tttttgagtt gcatcgccat gcgagagaga actgttggca tattaaatac atcctgtctg
    20821 ctgcgaacaa tatctgtagt gaatatcaat atccattgaa ggcgtcagta tggctatttt
    20881 ctcgatgaat aaaaatagct ttattcagcc accgcattca taacttatat attttattta
    20941 ttaattggct
//
LOCUS       DAZGTR010000076        20685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGTR010000076 DAZGTR010000000
VERSION     DAZGTR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.076"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(145..831)
                     /locus_tag="ACHLCY_003111"
     CDS             complement(145..831)
                     /locus_tag="ACHLCY_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFP0370318.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1412..2200)
                     /gene="phoH"
                     /locus_tag="ACHLCY_003112"
     CDS             complement(1412..2200)
                     /gene="phoH"
                     /locus_tag="ACHLCY_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP0370319.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2819..4090)
                     /gene="efeB"
                     /locus_tag="ACHLCY_003113"
     CDS             complement(2819..4090)
                     /gene="efeB"
                     /locus_tag="ACHLCY_003113"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP0370320.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQDEKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(4096..5223)
                     /gene="efeO"
                     /locus_tag="ACHLCY_003114"
     CDS             complement(4096..5223)
                     /gene="efeO"
                     /locus_tag="ACHLCY_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP0370321.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(5281..6111)
                     /gene="efeU"
                     /locus_tag="ACHLCY_003115"
     CDS             complement(5281..6111)
                     /gene="efeU"
                     /locus_tag="ACHLCY_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP0370322.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(6777..8285)
                     /gene="putP"
                     /locus_tag="ACHLCY_003116"
     CDS             complement(6777..8285)
                     /gene="putP"
                     /locus_tag="ACHLCY_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP0370323.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8444..8653)
                     /locus_tag="ACHLCY_003117"
     CDS             complement(8444..8653)
                     /locus_tag="ACHLCY_003117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370324.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8708..12670
                     /gene="putA"
                     /locus_tag="ACHLCY_003118"
     CDS             8708..12670
                     /gene="putA"
                     /locus_tag="ACHLCY_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0370325.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12710..13348)
                     /gene="rutR"
                     /locus_tag="ACHLCY_003119"
     CDS             complement(12710..13348)
                     /gene="rutR"
                     /locus_tag="ACHLCY_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP0370326.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            13636..14727
                     /gene="rutA"
                     /locus_tag="ACHLCY_003120"
     CDS             13636..14727
                     /gene="rutA"
                     /locus_tag="ACHLCY_003120"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP0370327.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            14727..15419
                     /gene="rutB"
                     /locus_tag="ACHLCY_003121"
     CDS             14727..15419
                     /gene="rutB"
                     /locus_tag="ACHLCY_003121"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP0370328.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            15431..15817
                     /gene="rutC"
                     /locus_tag="ACHLCY_003122"
     CDS             15431..15817
                     /gene="rutC"
                     /locus_tag="ACHLCY_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP0370329.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            15825..16625
                     /gene="rutD"
                     /locus_tag="ACHLCY_003123"
     CDS             15825..16625
                     /gene="rutD"
                     /locus_tag="ACHLCY_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP0370330.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            16635..17225
                     /gene="rutE"
                     /locus_tag="ACHLCY_003124"
     CDS             16635..17225
                     /gene="rutE"
                     /locus_tag="ACHLCY_003124"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP0370331.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            17236..17730
                     /gene="rutF"
                     /locus_tag="ACHLCY_003125"
     CDS             17236..17730
                     /gene="rutF"
                     /locus_tag="ACHLCY_003125"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP0370332.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            17751..19079
                     /gene="rutG"
                     /locus_tag="ACHLCY_003126"
     CDS             17751..19079
                     /gene="rutG"
                     /locus_tag="ACHLCY_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP0370333.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(19162..19335)
                     /gene="ymdF"
                     /locus_tag="ACHLCY_003127"
     CDS             complement(19162..19335)
                     /gene="ymdF"
                     /locus_tag="ACHLCY_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0370334.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            20103..20176
                     /locus_tag="ACHLCY_003128"
                     /pseudo
     CDS             20103..20176
                     /locus_tag="ACHLCY_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813257.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            complement(20266..>20685)
                     /locus_tag="ACHLCY_003129"
     CDS             complement(20266..>20685)
                     /locus_tag="ACHLCY_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370335.1"
                     /translation="RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYVDQQKMY
                     DRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYAD
                     STLSLAQQSAMVKSVERDMKIAVDRFNREYNKKVGVD"
BASE COUNT         4915 a         5584 c         5255 g         4931 t
ORIGIN      
        1 gtgagcgggc tgcccgcagg gcgagcgaag cgagtcaatc ccccctcacc gccatcttta
       61 aagaagagct cgtacgaagg tacgggcttt ttttcgtata ttgcacacac cggggggatg
      121 agaagccccg accgggatgc ttagttaatg caccggttca ctctttgtcg ccgcgactaa
      181 tgggcgaatt ttgtcacgag tctcccagac cggaggaatt tcctccaatt tagtgacaga
      241 agctcgtaca aaaggcaccc gtgcaagagt cataattgta tccaaccaac ggtcacgaat
      301 aatgcgatcc gccctttcat gactcgggtc atccagctca ataacacaga ctattttatt
      361 gttcttcttt gaaaccagaa cataatccat tgccatgcca ttataaattg caaagaaacg
      421 ctttctttct tcttcgctaa actcaggttg ttctggaaag ttgatcaagc gccccagaga
      481 gacctggcaa tggatatgac aataatgtcc aaaccatttt tccagattat cgaaatacat
      541 tttttcacaa cgcgtgatta accactcttt tctttccaat aaaatacctt cggcaaccat
      601 tccctgaaat aactcatctc gctcttgcca acgagagtca cgataggttt tctgcggtat
      661 atagctcctt gtcgacgcct tagcggcagg ggaagttgca gatgaaggga cgggttgcga
      721 tttagcgatg attaccgatg aggcaacttt tcggagtgtc aattttctgg caacagcaaa
      781 accggcgata aatgcaattg tcaaacaagt tacaaaagcc ataaagctca taattaattt
      841 tcacctatcc atgttgatta tatttcatta ccaatgttac tcgctgcagc gtgttacatg
      901 ccaacaatgc aacgatcaat atatagttag tatcatgttt tgattattac aaccatccct
      961 atttagccat tgcggcttgc cttccacaaa aaaataacaa cacacaatat tagcaaagaa
     1021 ttaattaaga ttaaccttac atttataata ttatccctaa agaataaatc agaggaatat
     1081 attcggtgtt aaacagtcag tcaaagtata gcccattgtt gattaaacct cataagcaaa
     1141 attggcacca gatctcctgt acaggttgaa tggccgaaaa aagataggca gatgctcatt
     1201 tttttggctt ctggaagaaa aactctccgc gctggcagta ttacactggt ctgatgctat
     1261 gacgaaaaat gcctggtaag agtatctaaa ccagactcca gttcagctta tcaacacgct
     1321 caaagtcgcc cccctgaacg ctaacctgaa gtgaaaatct gttgaaataa aacaaagccc
     1381 ggttcgcccg ggctctgcac cgataataca cttaactgta ggcatgcagc gtacgttggc
     1441 aaagtgccga acgtacgcag tcctctttac cgaaccggac gatcccaacc atttcatctt
     1501 cttcgaaacg ttccagcgcg tcacttaatc cggagcacac gccgcgaggc aaatcgcatt
     1561 gcgtgatatc accgttgacg ataaccgtca cgttctcccc gaggcgggtt aaaaacattt
     1621 tcatttgcgc ggcagtcaca ttctgcgcct cgtcaagaat gacgactgca ttttcaaagg
     1681 tacgtccacg catataggcg aacggcgcaa tttccacctt ccctatttcc ggtcgcaggc
     1741 agtactgcat aaaggaagcc cctaagcgcc ggaccagcac gtcgtagacc gggcgaaaat
     1801 agggagcaaa cttttctgcg atatctccag gtaagaagcc aagatcttca tcggcttgca
     1861 gaactggacg ggtgacgata atcctgtcga catccttatg tatcagggct tctgccgctt
     1921 ttgctgcgct gatccaggtt tttccgcacc cagcttcgcc cgtggcgaat atcagctgct
     1981 tactctcaat agccttcaga tagtgcaatt gcgcttcatt tcgcgcgagg atgggcgaag
     2041 tatcgcgact gtcgcgggcc ataccaatgg cttctacgcc gcccatctgc acaagcgagg
     2101 tgaccgattc ttcttcacgc tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg
     2161 cctcgcgacg agctttgatc actgcttttt gtcttcccat ggagagcacc ttgagttgtt
     2221 tgtattcatc acacgcgccg ttggcagcgc gattatgcgc acgaacatca gagggttggc
     2281 ttccttgtaa gccatagttt gcttttggat aaaatgccga aaaacggcta cgcacaccgt
     2341 ttgcggcgtc ggtaacacga gtaaagagag gaagtgagaa aaatacagag tgacgaagtt
     2401 gctaccggag gagaaatctc cgcaagtggt ggagcgtagc ttaacaagac attgtccaga
     2461 acaggacgtt accatccgcg atctccatag tgactgacta tcactgccgg gaacttccgc
     2521 tgctacttaa taattacaac agatctcgca ttcattgcaa caatatattc acttatattt
     2581 aaccataaaa caccatttca gtgacattag tttctactgg aaagatgaca gagtgatgac
     2641 agatatgaaa aaagctgtgt gctttcagca ggattcgcat caggcatctg cgcacgaacg
     2701 ccggatgcgg cgtgaacgcc ttatccgcct accgaacccg caccgattgt aggcataaga
     2761 agacgcgcaa gcatcgcatc aggcatctgc gcaagaacgc cttatccgtc tataaacatt
     2821 aaacccgcaa taacgcgctt ccgagataat cgttcgcgtc cttcactccc ggcagcgcaa
     2881 aaaaataacc gccgccgata ggtttaacgt attcctccag cgcttcgcca ttgagccttt
     2941 tttgtactgt caggaagcct ttttccagat cgtgttggta acagacaaac agtagcccca
     3001 tatccagttg cccggagttg gtgacgccca gtgaataact gtagccacga cgcagcatca
     3061 ggctggactc actctccgcc gtgcggggat tcgccagccg gatatggctg tccagcgcaa
     3121 tcaccttccc ttccggatcg ctggcgtaat cgggaacatc atgctcatgc tgcattccca
     3181 gcggcgcacc ggtttgctta tcacgaccaa aaatcgtctg ctgttctttc agcggagttc
     3241 tgtcccaaaa ttccactcga aactgaatca agcgtactgc ctgatagctg ccaccgattg
     3301 tccacgctgg ctcctgctga tctgccgtta cccacaccac tttttgcatc aacttctcat
     3361 cctggctatc gggattggca gtgccgtctt tgaaacccag caaattaatc ggcgtctctt
     3421 tgcctttact acgcgccgcg tgatcggaaa taaacccttc ccgcttccag cgcacgctga
     3481 gcaaatccgg cgtatgtttg atgatatcgc gcagtgcatg gatcaccgtg tcctgggtgt
     3541 tggcgcaaat ttgtagcaac acatcaccat gacataacgc cgcatccagc gagtcgttgg
     3601 gaaaacgcgt catcttctgc aactttttcg gcatctgtgg cgcaaggcca aagcgctcat
     3661 caaacaatga gtgacccacc gataacgtga tggtgagatt atcgggcgca atgtagccgc
     3721 caagaatgcc ggaatcgagt ggtggcaggc gcggatttgg cgtttctggt gccgctccgc
     3781 cctgagtcag aaaagcaaaa cgctgagtca acaagcgaaa caaccgttca agatcggctt
     3841 tatcgctggc aagcacatca aacgccacca gcatcattgc ggcctgttgt ggcgtcagga
     3901 tccctgcctg atgctcacca taaaacggct gtttctcatt gcgtgcatcc ggtgaaagcg
     3961 tacccggcgc actttgcgtt ttttgtgcat gagcgaccgg acaacttccc gccagcgcca
     4021 gcgcaccaat gcctttcagt aaacgtcggc gtgacggttc attcacgccg tttttatctt
     4081 catactgcat aatgcttaat ccaatcccag cacaccgcga agttgcgcca gatcttccgc
     4141 cagcgcagta atcggtcctt tcagtgcatt ccggtcggca tcggtcaatt tgtcgtaggt
     4201 ttcaaaaccg tctttagtac ggtatttcgc cagaatggta tcgacctttt taaagttggc
     4261 atcgactttt gccaacagtt ccgggttggc tttttgcagt tgtggacgca gcagatcgac
     4321 aattttctgc gagccttcaa cgttagcctg gaaatcccac agatcggtgt ggctgtagcg
     4381 atcttcttca ccgctgattt tgctggctgc cacttcctca atcagtccgg ctgcaccgcc
     4441 gaccactttt gaaggtggga aagccagttc actgatgcgt ttttgcaaat cgaccacatc
     4501 ggtataaagc tggtcagcat actgatccat gcctttggtg gtgttgtcgc caaacaatgc
     4561 tttttccaga cggtggaaac cggtgaattt tggatcagcg gctttttgct cgtaatcatc
     4621 ttcacgggcg tcaatgctgc catccagatc ggagaacagt tcagcaatcg gttcaatgcg
     4681 ctcatagtgc tggcgcgtcg gcgcatacag tgctttcgct ttttcgatat cgcctgcttt
     4741 aatcgcgtcg gtaaaggctt tggtgtcggt caccagctgc gtggtttccg ccatgacata
     4801 cgctttatat gcagtaattg caccaccaag acttaacagc gcatcacttt gcgccgcatc
     4861 cgccgttgcc tcacctttga cgatcaactt ccctttcggg ttagtcagca gaccgcaggt
     4921 catatcgtat tcgccaggct gtaaattcgc cgtcattttc tggctaaaac caggggcgat
     4981 attttcccgc tcttccacca ccatcacgcc tttgaggatc tcccactcca gcgccttctg
     5041 gctatggttc tgaataataa actgtgtttt cccggcgtta accgtaatgg tcatcggttc
     5101 gcactgctta tccgtcacgg tcactttgac ctgcgtcaca tcagcggcgt tagccataaa
     5161 agcagaagaa aacagcgcag ccacgctcaa ctgcaatgcg ttacggcgga agttaatggt
     5221 catgacgaga ctatcccttt aaagtatgtt gtaactaagc aagagtttgc gtcgtatttg
     5281 ttacgcggag cgagacgctg tcgcccctgc gcgtggtggc agagcaaatg ccaccagcgc
     5341 cgggatgaga taaataaacc agacggcgac ttcgctgacg ctcggcgctt cctgatagcc
     5401 aaaaatccct tccatcagcg tgccaaacag cgagtgagtt gagagcaccg cactcatatc
     5461 gaaggcgatt tcctgaaagt ggttccacaa tccggcttca tgaaatgcgc gaatggcacc
     5521 agctgccagc cctgcggcga cgaagagaat aaacaggctg gtccatttaa aaaatgcacc
     5581 aagattgagg cgaataccgc cccagtagag caagaagcct agcaccacgg cagtagcaag
     5641 accgagcatt gcacccagcg gcggccagat cccgacatct tgttgaaatg ccgccagcag
     5701 gaaaaagacc gactccagcc cttcccttgc aacggcaaaa aagaccatca tcaccagcgc
     5761 ccagccatga tgatttccac gctgcaatgc gctatcgact gcctgctcca gttgtacttt
     5821 gacgttgcgc gacactttgc gcatccagaa aaccatccag gtaaggatca ccacggcgat
     5881 caccgccacg ataccttcaa acagttcctg ctctttttgc ggaaattcgc cggtggtttc
     5941 gttaatgaag atccccaagc ccaggcacaa cgcagcggca agcaacacgc caatccacat
     6001 cacaccaatc catcggcctc gctgggtacg cttaagatag ctggcaatca aactgacaat
     6061 cagcgcggct tcaagtcctt cgcgcaacat aatgagaaac ggaacaaaca tgccacccac
     6121 ccttaaacgt ttttctgagt caatctatgc aaagaaaagt aaatcgcagt gatagcgatt
     6181 atcattacgg taaagaaaaa tacaagcgga atgtttataa aatgatgttg aaatgtaaac
     6241 aaagagaggt aatgccaggg gatataaacg attattccag gtacaaagat gggattgttt
     6301 acaacgagcc atgtgttctt gccggatgcc gcgtaaacgc cgtatccagc cgacaaaacc
     6361 gtcctgactc aatacatcaa aacttccatg taaaactgat aagcgggtgt atcaagcaat
     6421 ttcgtccatc ctcgattatc aaatctaact ttttgcccgg aacataagaa cagcgcgggt
     6481 gttggtggcg tcaaaatcgc tgaaacgttc cccgaaggcc ggggcagccc acatggatgt
     6541 gggctgaggg cgcgtcttac aggacgttac ctcgcgcccg acccggtagc cgaaggggat
     6601 aagctgaagg taccgcgcag cggcgatttt gttcgccaga gcccgggggt gcagggggcc
     6661 gcggcgattg gccgccccct gcgcgctccc tgcaccattc aatttatatg gcgaggcaca
     6721 ttaagggagc gcaacccgct gctgaagcca aacaaaaccg ccgcaggcta agtcccttag
     6781 ctttcctgca accgtgacgg cggagccgaa tgatagtgcg catcggcctc ggcaaagcgt
     6841 ttttgcatcg ccgctgacgg cgctttaccc agcaaactaa acactacaat cccgatactg
     6901 ccgaagatga agcccggaat aatttcgtag agtcccagcc agccgaactg cttccagacg
     6961 ataaccgtca gcgcaccgat gatcatcccc gccagcgcac cgttgcgcgt catgcgtgac
     7021 cacatcaccg agaacagcac caccggacca aacgccgcgc caaagcctgc ccacgcgtag
     7081 ctcactaagc ccagcacgcg gttttccgga tttgccgcca gcgcaatcgc caccagcgcc
     7141 accaccagca ccatcacacg tcctacccac accagctctt tctggctggc ctgtttacgc
     7201 agaaacgctt tgtataaatc ttcggtaatt gcactggaac ataccagcag ctggcaactt
     7261 aaggttgaca ttaccgccgc cagaatcgcc gacagcagaa tcccggcaat ccacgggtta
     7321 aacagaattt gcgccagttc gataaacacg cgctcggcgt tctggtttac cgcgcctgct
     7381 accgccgggt gctcattaaa gtaagcaatc ccaaagaagc caacagccac cgcccctgcc
     7441 aggcagagga tcatccaggt catactgata cgacgcgcat ggacaatgct gtggtgagag
     7501 tctgccgcca taaaacgcgc caggatgtgc ggctgcccga agtaacccag cccccaaccc
     7561 atcagtgaga taatggcgac aaagttcagc cctttgagca tatcaacgtt ttcgatgctc
     7621 ttttgtttga tcacttccag cgagtcacca aagccaccga cactgataat gacgataacc
     7681 ggcgtcagga tcagggcaaa aatcatcagg ctggcctgta cagtgtcagt ccagctcacc
     7741 gcgaggaaac cgccaataaa ggtgtaaagg atcgtcgccg cagctcccgc ccacagagcc
     7801 gtttcgtagc tcatgccaaa ggtactttca aacagacgcg cgcctgccac aatgcccgaa
     7861 gcgcaataaa tggtgaagaa cagcaaaatc accagcgcgg agataatgcg caaaatgcgg
     7921 cttttatctt caaagcgccc ggtgaaataa tccggcaatg ttaaggcgtt attgttgtat
     7981 tcggtatgca cacgcaaccg ccccgccacc agcttccagt tgatccacgc gcctaatgtc
     8041 aggccaatgg cgatccaact ttcggaaatc ccggaaagaa aaaccgcgcc cggcaacccc
     8101 attaacagcc agccgctcat atccgacgca cccgccgata atgccgtcac gaatggccca
     8161 aggctacgac cgcccagaat atagtcgtca aagtttttcg ttgatcgcca ggcgataaac
     8221 ccaatcaata tcatgccaaa gatatagaca caaaatgtca ccaacatcgg tgtgctaata
     8281 gccatctaaa gtctccaaaa aattattatc ggcaatgtcg aaacttgccg ttatatctgc
     8341 caccggaacg gggtaacaga gtttatgttt taccagggcg accgtatcct gccggaagcg
     8401 ctggttattc acaatcgatt taacacacca tttacattaa attttagtgc tcagcgacac
     8461 tatttttcat caggttgcac tctctcacat tttttgcggt tgcacctttc aaaaatgtta
     8521 actgccgcag agaaaaagtc tgagttattt ttttaatccc tgtcatatcg atttctttta
     8581 ttaacatttc attcattttt aagcttgcta cgcatgtcac atttaacatg gttgcacaaa
     8641 gttgcaacat catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag
     8701 taatggcatg ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat
     8761 taagtctgcc gcgacacgta tcgatcgcac gccacactgg ttaattaagc aggcgatttt
     8821 ttcttatctc gaacaactgg aaaacagcga tactctaccg gagctacctg cgctgctttc
     8881 tggcgctgcc aatgagagcg atgaagcacc gactccggca gaggaaccac accaaccatt
     8941 cctcgacttt gccgagcaaa ttttgcccca gtcggtttcc cgcgccgcga tcacagcggc
     9001 ctatcgccgc ccggaaaccg aagcggtttc tatgctgctg gaacaagccc gcctgccgca
     9061 gccagttgct gaacaggcgc acaaactggc gtatcaactg gccgataaac tgcgtaatca
     9121 aaaaaacgcc agtggtcgcg caggtatggt tcaggggtta ttgcaggagt tttcgttgtc
     9181 atcgcaggaa ggcgtggcgc tgatgtgtct ggcggaagcg ttgttgcgta ttcccgacaa
     9241 agccacccgt gatgcgttaa ttcgcgacaa aatcagcaac ggtaactggc aatcacacat
     9301 tggtcgtagc ccgtctttgt ttgttaatgc cgccacctgg gggctgctgt ttactggcaa
     9361 actggtttcc acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa
     9421 aagcggtgaa ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca
     9481 gttcgtcact ggcgaaacca tcgcggaagc gttagccaat gcccgcaagc tggaagagaa
     9541 aggtttccgt tactcttacg atatgcttgg cgaagctgca ctgactgccg cagatgcaca
     9601 agcgtatatg gtttcctatc agcaggcgat tcacgccatt ggtaaagcgt ccaatggtcg
     9661 tggcatctat gaaggtccgg gcatttcaat caagctgtcg gcgctgcatc cgcgttacag
     9721 ccgcgcccaa tatgaccggg taatggaaga gctttaccca cgtctgaaat cactcaccct
     9781 gctggcgcgt cagtacgata ttggtatcaa cattgacgcc gaagaggccg atcgtctgga
     9841 gatctccctc gatctgctgg aaaaactctg tttcgagcct gagctggcag gctggaacgg
     9901 tatcggtttt gttattcagg cttatcaaaa acgctgccca ctggtgattg attacctgat
     9961 tgatctcgcc acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg
    10021 ggatagtgaa atcaagcgtg cgcagatgga cggccttgaa ggctatccgg tttatacccg
    10081 caaggtgtat accgacgttt catatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa
    10141 tctaatctac ccgcagttcg cgacgcacaa cgcccatacg ctggcagcga tttatcaact
    10201 ggcaggtcag aactactatc cgggtcagta cgagttccag tgcctgcatg gtatgggcga
    10261 gccactgtat gagcaggtca ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg
    10321 tatttatgct ccggtcggta cccatgaaac gctgttggcg tatctggtgc gtcgcctgct
    10381 ggaaaacggt gctaacacct cgtttgttaa ccgtattgcc gacacctctt tgccactgga
    10441 tgaactggtc gccgatccgg tcactgctgt agaaaaactg gcgcaacagg aagggcaaac
    10501 tggattaccg catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa
    10561 ctcggcaggg ctggatctcg ctaacgaaca ccggctggcc tcgctctctt ctgccctgct
    10621 caatagtgca ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg
    10681 tgagatgtcg cccgttatta accctgcgga accgaaagat attgtgggct ttgtgcgtga
    10741 agccacgccg cgtgaggtag aacaggcgct ggaaagtgcg gttaataacg cgccaatctg
    10801 gtttgccact cctccggttg aacgcgcggc gattttgcat cgcgctgccg tgctaatgga
    10861 aagccagatg cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa
    10921 cgccattgcc gaagtgcgcg aagcggtcga ttttctccac tactatgccg gacaggtgcg
    10981 ggatgatttc gctaacgaaa cccaccgtcc attagggcct gtggtgtgta tcagtccgtg
    11041 gaacttcccg ctggctattt tcaccgggca gattgctgcc gcactggcgg caggtaacag
    11101 cgtgctggca aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt
    11161 gctggaagcg ggtgtaccgc caggcgtggt gcagttgctg ccaggtcagg gggaaaccgt
    11221 gggcgcgcaa ctgacgggtg atgatcgcgt acgcggggtg atgtttaccg gttcaaccga
    11281 agtcgctacg ttactgcagc gcaatatcgc cagccgcctg gacgctcagg gtcgccctat
    11341 tccgctcatc gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac
    11401 cgaacaggtc gtcgtggatg tactggcctc ggcgttcgac agtgcgggtc agcgttgttc
    11461 ggcgctgcgc gtgctgtgcc tgcaagatga gattgccgac cacacgttga aaatgctgcg
    11521 cggcgcaatg gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc
    11581 ggtgattgat agcgaagcga aagccaatat tgagcgccat attcagacca tgcgtagcaa
    11641 aggccgtccg gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag
    11701 cggcaccttt gtcgccccga cgctgatcga actggatgac tttgccgaat tgcaaaaaga
    11761 ggtctttggt ccggtgctgc atgtggtgcg ttacaaccgt aaccagctac cagagctgat
    11821 cgagcagatt aacgcttccg gctatggtct gacgcttggt gtccatacgc gtattgatga
    11881 aaccatcgcc caggtcactg gctcggccca tgttggtaac ctgtatgtta accgtaatat
    11941 ggtgggcgca gtggtgggtg tgcagccatt cggcggcgaa gggttgtccg gtaccgggcc
    12001 gaaagcaggc ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct
    12061 ggcagtgacg ctcgcgcgtc aggatgcaga gtatccggtc gatgcacagt tgaaagccgc
    12121 attgactcag ccgctaaatg cactgcggga atgggcagca aatcgtccag aattgcaggc
    12181 gttatgtacg caatatggcg agctggcgca ggcaggaaca caacgattgc tgccggggcc
    12241 gacgggtgaa cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga
    12301 tgatgagcag gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca gccaggtact
    12361 gtggccggat gacgcgctgc atcgtcagct agtaaaggca ttgccatcgg cagtcagcga
    12421 acgtattcaa ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg cggtgatctt
    12481 ccacggtgat tcggatcagc ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc
    12541 aattgtttcg gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg aacggctgta
    12601 tatcgaacgt tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac
    12661 tataggttaa acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc
    12721 gaatcccctc aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat
    12781 cgcgcaacgt cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag
    12841 tggaagccca aatcatgaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt
    12901 tgacccaacc ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca
    12961 gttcatccat taacagcggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct
    13021 gcggataatc gcgggagact tccagcttca gacggatgta ctcttttatc gccgccagcg
    13081 gagcgaaatc ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca
    13141 gcacggcaat atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa
    13201 cacccgccaa ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag
    13261 tgtccagtgc tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac
    13321 ccgttgtttt cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga
    13381 tagcaaaacc cccgcccggc ctcgaccttc tgcactctca tcgcgcacag atgcatgttt
    13441 tatgtgcaac tgttttgacc gtttagtcca ctttttacca gatattttag acaacacaat
    13501 gttaacacat taataacatt cactttttaa aaactggcat ccgctttgca aacaagccat
    13561 tacgcggctg aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac
    13621 gaagagaggt tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc
    13681 atttcgaccc acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa
    13741 aaagcggagc actaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc
    13801 ggcaaaactg agttctggga tcacaacctt gagtcgttca ccttgatggc ggggctggcg
    13861 gccgtcacct cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca
    13921 atcgtcgccc gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac
    13981 ctcgtgactg gctggcaaaa gcccgagtat gagcagatgg ggatctggcc tggcgatgac
    14041 tatttctccc gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg
    14101 ggcacgggga aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt
    14161 ccgcaaccga gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg
    14221 ttctccgccc agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacgcccacg
    14281 gctttcgccc cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg gcgcgacgtt
    14341 ggttcttatg tgttgtttat ggtgattgcc gatgaaaccg acgatgccgc tcgcgccaaa
    14401 tgggaacact acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt
    14461 cagaaagata cccgctccgg tactgatacc aacgtccgtc agatggccga tcccacttcg
    14521 gcggtaaaca tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta
    14581 gatgaagtcg caagcgtgcc tggtgccgaa ggcgtgctgt tgaccttcga tgattttctg
    14641 tcgggaatcg aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatcta
    14701 cctgcgctga ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta
    14761 ccttcgatcc gcagcaaagt gcgctgatcg tggtggatat gcaaaacgct tatgccacgc
    14821 caggcggcta cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca
    14881 acattcaaac cgccgtgacc gcagcgcgag cggcagggat gctgatcatc tggtttcaaa
    14941 atggctggga tgaacagtat gtcgaagctg gcggccccgg ctcaccgaat tttcataaat
    15001 cgaacgccct gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag
    15061 gctcctggga ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc
    15121 cgaagccgcg ctacagcggt ttcttcaata caccgctgga cagcattttg cgcagccgtg
    15181 gaatacgcca tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac
    15241 gcgatggctt ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg
    15301 ggccggaatt tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca
    15361 gcgacgtcga aacgttctgc gacgcgcttt ctcccacatc ctttgctcgt atcgcttaag
    15421 gagtttaacg atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc
    15481 cttcgttccc ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga
    15541 tcaacataat aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga
    15601 aattatccgc aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag
    15661 catctttatt accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt
    15721 tccgggtgat aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct
    15781 ggtggaaatc gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct
    15841 cacctccccc ttatgctgat gcgcccgtag tggtgttgat ttcgggtctt ggcggtagcg
    15901 gcagttactg gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg
    15961 accagcgcgg caccggcaat aatcccgaca cgctggcaga agattacagt atcgcccaga
    16021 tggcagcgga actgcatcag gcgctggtag ccgcagggat tgagcattac gcggtggtcg
    16081 gccatgcgct cggtgcgctg gtgggaatgc agctggcgct ggattatccc gcgtcggtaa
    16141 ctgtgctgat cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc
    16201 aggttcgcga acgattactg tatagcggcg gcgcgcaggc atgggtggaa gcgcagccgt
    16261 tgttcctcta tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg
    16321 cgctggcact ggcgcatttt cagggcaaaa ataatttact gcgtcgactt aacgccctca
    16381 aacgcgctga ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg
    16441 ccagtgatga tctgctggtg ccatcagcat gttccagtga gcttcatgcc gccctgcccg
    16501 atagccagaa aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa
    16561 cgtttaatgc tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc
    16621 tgtaaggaat tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ttgttcaccg
    16681 atgcccgcac tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacgggaga
    16741 tttatgccct gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt
    16801 ttatccgcac ggcagaagga aaagaacgtc tgcgcccggc actttccagc ggcaatctgc
    16861 aaaaaaccct gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac
    16921 ggttaccgca actgtttcct cacggcgatg cccgcagttg gtttacctcc agcccacaac
    16981 ttgccgaaga aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgcct
    17041 gccgtgcgct gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg
    17101 acgccttttt tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg
    17161 atagcagcaa actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt
    17221 tgtaaggagc aaaccatgaa cattgtcgat caacaaacct ttcgcgatgc gatgtcctgt
    17281 atgggcgcgg cagtcaatat catcaccacg gatggtccag ccgggcgcgc cgggttcacc
    17341 gccagcgcgg tctgcagcgt gaccgacacg ccgcctacat tactggtgtg cctgaatcgt
    17401 ggggcgtccg tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc
    17461 gccgggcagg agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt
    17521 gccgccgccc gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt
    17581 tcgtttgact gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc
    17641 gccatcgaag cgattcatcg tcacgccaca ccctacgggc tggtgtggtt cgatcgcagt
    17701 tatcacgcgc tgatgcgccc tgcttgttaa cctcctaaag gagacagctg atggcaatgt
    17761 tcggttttcc tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgt
    17821 atgaacgatt gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt
    17881 ttggcgcgac ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa
    17941 tgtcggggat cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc
    18001 tcggttccag cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg
    18061 gcattaaccc gaatatcagc attgcgctgg gcgggattat cgcctgtggg ctggtttata
    18121 ccgtcattgg cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc
    18181 cggtggtgac gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga
    18241 aaagtgtatc ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg
    18301 ggctggtggc ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggtttaa
    18361 ttgtcgcctg tctgctgtat ggcgtgatga ccaacctttt agggctggga aaagccgtcg
    18421 attttactct cgtcagccat gctgcctggt tcggtttgcc acatttttcc actcctgctt
    18481 ttaatagtca ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg gcggaaaact
    18541 taggtcatct caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc
    18601 gggcattcgt cggtgatggg ctggcgacga tgctttctgg ttctgtcggt ggcagcggcg
    18661 taaccaccta cgccgagaat atcggtgtaa tggccgtgac aaaagtctac tcgacgctgg
    18721 tgtttgtcgc tgctgccgtc atcgccatgc tgttgggctt ttcaccgaaa tttggcgcgc
    18781 tgattcatac cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga
    18841 ttgccgtcgc aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta
    18901 atttgattat ggtcgcagta accttagtgc tgggcgcggg tgattttgct ctcacgctgg
    18961 gcggttttac gttgggaggg attggtacag caacctttgg cgcgatttta ctcaatgcgt
    19021 tgttaagcag aaagttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa
    19081 cagaaaaccg ccgggattac cggcggtgat gactttttga cgagcctaag aaaaaaatgc
    19141 attcgtcatt gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt
    19201 tttacctgct tcagatgcgc gctgcgggtc attcttgaaa ttacccccgc tgtgctgtcc
    19261 acctttttta cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc
    19321 tcgatggttt gccattactg acctccgtct gggtatagac atcatattgc atagaattaa
    19381 cgcgtcgtat ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc
    19441 gggcagaccc gcgtttggta atatcctgca acagcttctt agcgagaata aatatcatct
    19501 caaataatta tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaatgtgtc
    19561 attttgcgac aaaattacgt gcttgtgaaa gttgttataa atcaaataag tggttgtgaa
    19621 atttgcactc tgaaaaggac gtcttatctt taaataagtg gtagcgaatc gctacggaat
    19681 agagataaca cgaggagtgg ttagaaatgg ctaaagttct ggtgctttat tattccatgt
    19741 acggacatat tgaaaccatg aagcattaaa aacacagaat taccgaagat cgtccaagag
    19801 catatacttt tcaatatgtt agacctcatt ttaaccatga agaaatcgat tttacaccgt
    19861 tctttatacg ttcttttttg ccccaaatat gccccaaaca accacacttt gccccaatag
    19921 cgccccaaag atttaagggg atttaagtat atatttcaga taattaacgc aagtttacga
    19981 tgtgaattta cgttaatttt ctgggtaggg tatttaccgt cattcgttca tcaatatttc
    20041 tgcatattga caaatacgcc tgagctatcg cgggagaaat gcaatgaaag cagcaaaagg
    20101 cgatgttaat caccctgctc atctctgtat taccgtcatt gtgacggcac tgataacgaa
    20161 gaaagacctc tgcaagggag gaatccctcg ccacctctga tgtgtcaggt atcctcaccc
    20221 tccccaatca cattagcccc gcacttccgt acggggccat ttctgttaat ccactcccac
    20281 ttttttgttg tactcgcggt taaaccgatc cacggcaatt ttcatatccc gctctaccga
    20341 tttcaccatc gccgactgtt gcgccagact gagagtactg tcggcgtaaa tggcatcacg
    20401 ctgtttacgc aaatccttca gtctcttccg ggtatcctgc ataagcccgt tcatcgacaa
    20461 ttttccgttg ttctcgtcaa tgaacgccgt tctttctgcg ccggtcagac tcttcagctc
    20521 tgcgtgatac tgcgcaacct ctgtcatccg gtcgtacatc ttctgctggt caacatacgg
    20581 catcacctca cctgaaattt tccccaggaa aggcacctgc tgttccggta tatcaatacc
    20641 gttcagcgat ttcaccgccg catccgtggt tttggaaatg aagcg
//
LOCUS       DAZGTR010000077        20302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGTR010000077 DAZGTR010000000
VERSION     DAZGTR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.077"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            56..1033
                     /gene="ybeQ"
                     /locus_tag="ACHLCY_003130"
     CDS             56..1033
                     /gene="ybeQ"
                     /locus_tag="ACHLCY_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFP0370336.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1103..1585)
                     /gene="ybeL"
                     /locus_tag="ACHLCY_003131"
     CDS             complement(1103..1585)
                     /gene="ybeL"
                     /locus_tag="ACHLCY_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP0370337.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1820..4402
                     /gene="leuS"
                     /locus_tag="ACHLCY_003132"
     CDS             1820..4402
                     /gene="leuS"
                     /locus_tag="ACHLCY_003132"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP0370338.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4417..4998
                     /gene="lptE"
                     /locus_tag="ACHLCY_003133"
     CDS             4417..4998
                     /gene="lptE"
                     /locus_tag="ACHLCY_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP0370339.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDKEQTSTTTDTPATPARVSTTLGN"
     gene            4998..6029
                     /gene="holA"
                     /locus_tag="ACHLCY_003134"
     CDS             4998..6029
                     /gene="holA"
                     /locus_tag="ACHLCY_003134"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP0370340.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVICQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6031..6672
                     /gene="nadD"
                     /locus_tag="ACHLCY_003135"
     CDS             6031..6672
                     /gene="nadD"
                     /locus_tag="ACHLCY_003135"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP0370341.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6696..7307
                     /gene="cobC"
                     /locus_tag="ACHLCY_003136"
     CDS             6696..7307
                     /gene="cobC"
                     /locus_tag="ACHLCY_003136"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP0370342.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPIPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7567..7884
                     /gene="rsfS"
                     /locus_tag="ACHLCY_003137"
     CDS             7567..7884
                     /gene="rsfS"
                     /locus_tag="ACHLCY_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP0370343.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7888..8355
                     /gene="rlmH"
                     /locus_tag="ACHLCY_003138"
     CDS             7888..8355
                     /gene="rlmH"
                     /locus_tag="ACHLCY_003138"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP0370344.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8386..10287
                     /gene="mrdA"
                     /locus_tag="ACHLCY_003139"
     CDS             8386..10287
                     /gene="mrdA"
                     /locus_tag="ACHLCY_003139"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP0370345.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10290..11402
                     /gene="mrdB"
                     /locus_tag="ACHLCY_003140"
                     /gene_synonym="rodA"
     CDS             10290..11402
                     /gene="mrdB"
                     /locus_tag="ACHLCY_003140"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP0370346.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11413..12501
                     /gene="rlpA"
                     /locus_tag="ACHLCY_003141"
     CDS             11413..12501
                     /gene="rlpA"
                     /locus_tag="ACHLCY_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP0370347.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12641..13852
                     /gene="dacA"
                     /locus_tag="ACHLCY_003142"
     CDS             12641..13852
                     /gene="dacA"
                     /locus_tag="ACHLCY_003142"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP0370348.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13963..14226
                     /gene="ybeD"
                     /locus_tag="ACHLCY_003143"
     CDS             13963..14226
                     /gene="ybeD"
                     /locus_tag="ACHLCY_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP0370349.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14327..14968
                     /gene="lipB"
                     /locus_tag="ACHLCY_003144"
     CDS             14327..14968
                     /gene="lipB"
                     /locus_tag="ACHLCY_003144"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP0370350.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15227..16180
                     /gene="ybeF"
                     /locus_tag="ACHLCY_003145"
     CDS             15227..16180
                     /gene="ybeF"
                     /locus_tag="ACHLCY_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP0370351.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16389..17354
                     /gene="lipA"
                     /locus_tag="ACHLCY_003146"
     CDS             16389..17354
                     /gene="lipA"
                     /locus_tag="ACHLCY_003146"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP0370352.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17455..17658)
                     /gene="tatE"
                     /locus_tag="ACHLCY_003147"
     CDS             complement(17455..17658)
                     /gene="tatE"
                     /locus_tag="ACHLCY_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP0370353.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17787..18575)
                     /locus_tag="ACHLCY_003148"
     CDS             complement(17787..18575)
                     /locus_tag="ACHLCY_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP0370354.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18668..19051
                     /gene="crcB"
                     /locus_tag="ACHLCY_003149"
     CDS             18668..19051
                     /gene="crcB"
                     /locus_tag="ACHLCY_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP0370355.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19105..19314)
                     /gene="cspE"
                     /locus_tag="ACHLCY_003150"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19105..19314)
                     /gene="cspE"
                     /locus_tag="ACHLCY_003150"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0370356.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19489..20049)
                     /gene="pagP"
                     /locus_tag="ACHLCY_003151"
     CDS             complement(19489..20049)
                     /gene="pagP"
                     /locus_tag="ACHLCY_003151"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP0370357.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFRDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5151 a         5113 c         5082 g         4956 t
ORIGIN      
        1 aaatggaaca cataaaaata taatatgttt tatctgcaac cataaggaaa tgataatgat
       61 tttcacgtca agttgctgcg ataatttatc aatagatgag attatcgaac gtgctgaaaa
      121 aggcgattgc gaggctcaat atattgttgg gttttattat aatcgcgata gcgcaattga
      181 ttctccggac gacgaaaaag ccttttactg gctgaagctg gccgctgagc aaggtcattg
      241 tgaagcacag tattccttag ggcaaaagta taccgaggat aaaagccgtc ataaagataa
      301 tgagcaagcc atcttctggc tgaaaaaagc tgccctacaa ggccatactt tcgcttccaa
      361 cgcccttggc tggacactgg atcgtggaga agcccccaat tataaagaag cggttgtctg
      421 gtatcagata gccgcggaga gcggaatgtc ttatgcgcaa aataatcttg ggtggatgta
      481 cagaaatggc aacggagtcg caaaagacta tgcgctggca tttttctggt acaaacaagc
      541 tgcattacaa ggccatagtg acgcgcaaaa caatctggcc gatctttatg aagacggaaa
      601 aggcgttgct caaaacaaga cactcgccgc attctggtat ttgaaaagcg cacagcaggg
      661 taatcggcac gcccagtttc aaattgcatg ggattataac gctggcgaag gggtggacca
      721 ggactataag caagcgatgt actggtatct gaaggctgcc gctcaggaaa gcgtcggcgc
      781 ttacgtcaac atcggttata tgtataaaca cggacaaggt gttgagaaag attatcaggc
      841 tgcctttgaa tggtttacga aagccgctga atgcaatgac gccactgcct ggtataacct
      901 ggccattatg tatcactacg gagaaggaag acctgtcgat ctccgacagg ctctcgacct
      961 gtatcgtaaa gttcagtcat ccggaaccag agatgtcagt caggaaattc gtgagactga
     1021 agatttactg tagagataaa aacaagggcc ggggaatttc attcggccca tattctcttt
     1081 attgctgtgc gaaatcagac tattacggct caaacgggcg tctctggaac tggtcatgac
     1141 cacatttcgg gcatagcgtc agcacttccg gtgtgtagat cgggagatgg aagtgacatt
     1201 tctcgcagac cagatttccc agcccgacca cttctccgct gtgataaacc ccatgatgat
     1261 tgaggtcctg gaaaacttcg cgccattcaa gctgcgtttt atcggtgata tctgccagct
     1321 cctgccataa gctttcttta atcacccgca taaagacgct gtcagattct tctttcaggc
     1381 tctcttcata gctcatggcg aactcttcca ggtcacgtct tacagctcgc gtcagctcat
     1441 cgacctcggt tcgcgttaac tcccctgttt ttattacgcg ctcgcgcgcc tgttccacca
     1501 gtgcgtcgat atcacgttcg ccattgcgca ggcgttcgct cagtgacgcg accagttcac
     1561 ggtaatattg agcaaccttg ttcatcgttt catctcccgg gtcgttaact gactataata
     1621 atagacgttc tttgttcctt gctttgctaa tacgcccaca aactctgtaa attaatgtat
     1681 gaataatccg ggcatcattt caacgtcgag tgcgcgaaag gctgttttga cgcggacgtt
     1741 tgggctatgc tatgcggatc tgaaaaacca cctcaacgct acatttgtag ccgtattgaa
     1801 aacaggacca ctggctgcca tgcaagagca ataccgcccg gaagagatag aatccaaagt
     1861 acagcttcat tgggatgaga agcgcacatt tgaagtaacc gaagacgaga gcaaagagaa
     1921 gtattactgc ctgtctatgc ttccctatcc ttctggtcga ctacacatgg gccacgtacg
     1981 taactacacc atcggtgacg tgatcgcccg ctaccagcgt atgctgggca aaaacgtcct
     2041 gcagccgatc ggctgggacg cgtttggtct gcctgcggaa ggcgcggcgg tgaaaaacaa
     2101 caccgctccg gcaccgtgga cgtacgacaa catcgcgtat atgaaaaacc agctcaaaat
     2161 gctgggcttt ggttatgact ggagccgcga gctggcaacc tgtacgccgg aatactaccg
     2221 ttgggaacag aaattcttca ccgagctgta taaaaaaggc ctggtatata agaagacttc
     2281 tgcggtcaac tggtgcccga acgaccagac cgtactggcg aacgaacaag ttatcgacgg
     2341 ctgctgctgg cgctgcgata ccaaagttga acgtaaagag atcccgcagt ggtttatcaa
     2401 aatcactgct tacgctgacg agctgctcaa cgatctggat aaactggatc actggccaga
     2461 caccgttaaa accatgcagc gtaactggat cggtcgttcc gaaggcgtgg agatcacctt
     2521 caacgttaac gactatgaca acacgctgac cgtttacact acccgcccgg acacctttat
     2581 gggttgtacc tacctggcgg tagctgcggg tcatccgctg gcgcagaaag cggcggaaaa
     2641 taatcctgaa ctggcggcct ttattgacga atgccgtaac accaaagttg ccgaagctga
     2701 aatggcgacg atggagaaaa aaggcgtcga tactggcttt aaagctgttc acccattaac
     2761 gggcgaagaa attcccgttt gggcagcaaa cttcgtattg atggagtacg gcacgggcgc
     2821 agttatggcg gttccgggtc acgaccagcg cgactacgag tttgcctcta aatacggcct
     2881 gaacatcaaa ccagttatcc tggctgctga cggctctgag ccagatcttt ctcagcaagc
     2941 cctgactgaa aaaggcgtgc tgttcaactc tggcgaattc aacggtcttg accatgaagc
     3001 ggccttcaac gccatcgccg ataaactgac tgcgatgggc gttggcgagc gtaaagtgaa
     3061 ctaccgcctg cgcgactggg gtgtttctcg tcagcgttac tggggcgcgc cgattccgat
     3121 ggtgacgctg gaagacggta ccgtaatgcc gaccccggac gaccagctgc cggtgatcct
     3181 gccggaagat gtggtgatgg acggcattac cagcccgatt aaagcagatc cggagtgggc
     3241 aaaaactacc gttaacggta tgccagcgct gcgtgaaacc gacactttcg acacctttat
     3301 ggagtcctcc tggtactatg cgcgctacac ttgcccagag tacaaagaag gtatgctgga
     3361 ttccgaagcg gctaactact ggctgccggt ggatatctac attggtggta tcgaacacgc
     3421 cattatgcac ctgctctact tccgcttctt ccacaaactg atgcgtgatg caggcatggt
     3481 gaactctgac gaaccagcga aacagttgct gtgtcagggt atggtgctgg cagatgcctt
     3541 ctactatgtt ggcgaaaacg gcgaacgtaa ctgggtttcc ccggttgatg ctatcgttga
     3601 acgtgacgag aaaggccgta tcgtgaaagc gaaagatgcg gcaggccatg aactggttta
     3661 taccggcatg agcaaaatgt ccaagtcgaa gaacaacggt atcgacccgc aggtgatggt
     3721 tgaacgttac ggcgcggaca ccgttcgtct gtttatgatg tttgcttctc cggctgatat
     3781 gactctcgaa tggcaggaat ccggtgtgga aggggctaac cgcttcctga aacgtgtctg
     3841 gaaactggtt tacgagcaca cagcaaaagg tgatgttgcg gcactgaacg ttgatgcgct
     3901 gactgaagat cagaaagcgc tgcgtcgcga tgtgcataaa acgatcgcta aagtgaccga
     3961 tgatatcggc cgtcgtcaga ccttcaacac cgcaattgcg gcgattatgg agctgatgaa
     4021 caaactggcg aaagcaccaa ccgatggcga gcaggatcgc gctctgatgc aggaagcgct
     4081 gctggccgtt gtccgtatgc ttaacccgtt caccccgcac atctgcttca cgctgtggca
     4141 ggaactgaaa ggcgaaggcg atatcgacaa cgcgccgtgg ccggttgctg acgaaaaagc
     4201 gatggtggaa gactccacgc tggtcgtggt gcaggttaac ggtaaagtcc gtgccaaaat
     4261 caccgttccg gtggacgcaa cggaagaaca ggttcgcgaa cgtgctggcc aggaacatct
     4321 ggtagcaaaa tatcttgatg gcgttaccgt gcgtaaagtg atttacgtac caggtaaact
     4381 cctcaatctg gtcgttggct aagcgcggga ggaagcgtgc gatatctggc aacattgttg
     4441 ttatctctgg cggtgttaat caccgccggg tgtggctggc atctgcgtga taccacgcag
     4501 gttccttcca ctatgaaggt catgatcctg gactcaggcg atccgaacgg gccattaagc
     4561 cgtgcggtgc gtaaccagtt acgtctgaat ggtgtcgagt tgcttgataa agaaaccacg
     4621 cgtaaggacg ttccatccct gcgtttgggt aaagtgagca tcgcgaaaga taccgcatcg
     4681 gtattccgta acggtcaaac agcagagtat cagatgatca tgacggtgaa tgcgaccgtg
     4741 ttgatccccg gccgtgatat ctacccgatt agtgccaaag tcttccgttc gttctttgat
     4801 aacccgcaaa tggcgttagc gaaagataac gaacaagaca tgatcgtaaa agagatgtac
     4861 gaccgtgctg ccgaacagct gattcgtaag ctgccaagca tccgtgctgc ggatattcgt
     4921 tccgacaaag aacagacgtc gaccacaacg gatactccgg caacgcctgc acgcgtctcc
     4981 accacgctgg gtaactgatg attcggttgt acccggaaca actccgcgcg cagctcaatg
     5041 aagggctgcg cgcggcgtat cttttacttg gtaacgatcc tctgttattg caggaaagcc
     5101 aggacgctgt tcgtcaggta gctgcggcac aaggattcga agaacaccac actttttcca
     5161 ttgatcccaa cactgactgg aatgcgatct tttcgttatg ccaggctatg agtctgtttg
     5221 ccagtcgaca aacgctattg ctgttgttac cagaaaacgg accgaatgcg gcgatcaatg
     5281 aacaacttct cacactcacc ggacttctgc atgacgacct gctgttgatc gtccgcggta
     5341 ataaattaag caaagcgcaa gaaaatgccg cctggtttac tgctcttgcg aatcgcagcg
     5401 tgcaggtgat ctgtcagaca ccggagcagg ctcagcttcc ccgctgggtt gctgcgcgcg
     5461 caaaacagct caacttagaa ctggatgacg ctgcaaatca ggtgctctgc tactgttatg
     5521 aaggtaacct gctggcgctg gctcaggctc tggagcgttt atcgctgctc tggccagacg
     5581 gcaaattgac attaccgcgc gttgaacagg cggtgaatga tgccgcgcat ttcacccctt
     5641 ttcattgggt tgatgctttg ttgatgggaa aaagtaagcg cgcgttgcat attcttcagc
     5701 aactgcgtct ggaaggcagc gaaccggtta ttttgttgcg cacattacaa cgtgaactgt
     5761 tgttactggt taacctgaaa cgccagtctg cccatacgcc actgcgtgcg ttgtttgata
     5821 agcatcgggt atggcagaac cgccggggca tgatgggcga ggcgttaaat cgcttaagtc
     5881 agacgcagtt acgtcaggcc gtgcaactcc tgacacgaac ggaactcacc ctcaaacaag
     5941 attacggtca gtcagtgtgg gcagagctgg aagggttatc tcttctgttg tgccataaac
     6001 ccctggcgga cgtatttatc gacggttgat atgaaatctt tacaggctct gtttggcggc
     6061 acctttgatc cggtgcacta tggtcatctt aaacccgtgg aaacgctggc gaatttgatt
     6121 ggtctgacgc gggtcacaat catccctaat aatgttcctc cgcatcgtcc ccagccggaa
     6181 gcgaacagcg tgcagcgtaa acacatgctt gaactggcga ttgccgacaa gccattattt
     6241 actcttgatg aacgcgagct aaagcgcaat gccccctctt acactgcgca aacactgaaa
     6301 gagtggcggc aggaacaagg gccggacgtg ccgctggcgt ttattattgg tcaggattca
     6361 ttgctgacct ttccgacctg gtacgaatac gaaacgatac tcgacaatgc acatttgatc
     6421 gtctgtcggc gtccaggtta cccacttgaa atggcgcaac cgcaatacca gcaatggctg
     6481 gaagatcatt tgacacataa cccggaagat cttcaccttc agcctgccgg taaaatttat
     6541 ctggctgaaa cgccgtggtt taacatctcg gcgaccatca tccgcgaacg tttgcaaaac
     6601 ggtgaatcgt gtgaggattt attgccggaa ccggtactga cttacattaa ccaacaaggc
     6661 ttgtatcgct gatacctgtc gttacggagg gcatgatgcg actgtggtta attcgtcatg
     6721 gtgaaacgca agcgaatatc gatggtcttt acagcggtca tgcgcccatc cccctgaccg
     6781 cgcgcggtat tgagcaagcg caaaatctgc atacactgct agatgatgtt tcctttgacc
     6841 tggttttatg cagtgaactg gaacgggcac agcataccgc gcgacgggtt ctcagtgacc
     6901 gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc gactgggaga
     6961 tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg tggtgcaatg
     7021 actggcagca tgctatcccc acgaacggtg aaggatttca ggcattttcg caacgtgtgg
     7081 aaagatttat cgcaaggctt agtgaatatc agcactatca gaatatttta atcgtcagcc
     7141 atcagggcgt actgagtctg ttaattgctc gtctaattgg catgcctgcc gaatctatgt
     7201 ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa aaattcgcga
     7261 cgctacgcgt cctcaatagc cgtgccatcg gggtcgagaa tgcatgactt ttctgttttt
     7321 ttacgggtaa gccgcaacga ccattgacag ccccgggcag gctgatattc tccgcagcca
     7381 gactttttcc gccagacacg actttgtaga aattgtttta caaaaatggc gatgcaatct
     7441 gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca attgtcccga
     7501 aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt cacccagggg
     7561 gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga tgacctcaaa
     7621 ggtcaggaca tcatcgcctt agacgttcag ggcaaatcca gcatcaccga ctgcatgatc
     7681 atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt tgtgcaggag
     7741 tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc cgactggatt
     7801 gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg tcgcctgtat
     7861 gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt gggaacgaaa
     7921 atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc gaaagatatg
     7981 cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga catcaagcgc
     8041 atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg cattgtcacc
     8101 ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct ggaacgctgg
     8161 aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt gtcgcctgcc
     8221 tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc ccatccgctg
     8281 gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac caaccatcct
     8341 tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa actacagaac
     8401 tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct ggtcgccttt
     8461 ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct gcaaattgtt
     8521 cgctttaccg attaccagac ccgctctaat gaaaaccgca ttaagctggt gcctatcgcg
     8581 cccagccgcg gcattatcta cgaccgtaac ggtatccctc tggcactcaa ccgcactatc
     8641 taccagatag aaatgatgcc ggagaaagtc gataacgtgc agcaaacgct ggacgctttg
     8701 cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga gcgcgcacgt
     8761 tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca agtagctcgc
     8821 tttgccgtca atcagtaccg ttttccgggt gtcgaagtta aaggctataa acgtcgttac
     8881 tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat caacgataaa
     8941 gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac gcatgatatc
     9001 ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac cggttatgaa
     9061 gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt accaccgcaa
     9121 gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat tgaaacgctg
     9181 ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg ggtgctggca
     9241 ctggtttcca cgcctagtta tgacccaaac ctgtttgttg acggtatctc cagcaaagat
     9301 tattccgcct tgctgaacga cccgaataca ccgctggtga accgcgccac acagggggtt
     9361 tatcctcctg cgtctacagt taaaccctat gtggcggttt cggcattaag cgccggggtg
     9421 atcacgcgca atacgacgct gtttgaccca ggctggtggc aactaccagg gtcggaaaaa
     9481 cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag atcgctggaa
     9541 gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga tcgcctctcc
     9601 gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc ggaagaacgt
     9661 tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc gtggtatcag
     9721 ggtgacacca ttccggttgg tatcggccag ggttactgga cagcgacccc aatccagatg
     9781 agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca tttgctgatg
     9841 agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc gcccgtcggc
     9901 gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt tgctaaccgc
     9961 cctaacggta cggcgcataa atactttgcc agcgcaccgt acaaaattgc ggcgaaatct
    10021 ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca caaaattgcc
    10081 gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa tccgcaagtg
    10141 gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac gctgatgcgc
    10201 cagatcctcg accacattat gctgggtgat aacaataccg atctgcctgc ggaaaatcct
    10261 gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa aaaacattct
    10321 gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg ctggtttaca
    10381 gcgccctggt tatctggagc gccagcggtc aggatattgg catgatggag cgtaaaatag
    10441 gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct ccacgcgttt
    10501 atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg gcggtagatg
    10561 ctttcggtgc catctctaaa ggcgctcaac gctggctgga cctcggtatt gttcgttttc
    10621 agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt atcaaccgcg
    10681 acgtttgccc gccatcgttg aagaacactg gcatcgcgct ggtgctgata tttatgccca
    10741 cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg ctttccggtc
    10801 tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca gtagtgctgg
    10861 tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag cgccagcgcg
    10921 taatgatgct cctggacccg gaatcagacc cactcggcgc gggctatcac attattcagt
    10981 ctaaaattgc tattggctcc ggtggattac gcggcaaagg ctggctgcat ggcactcagt
    11041 cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta ctggcggaag
    11101 agctgggatt agtgggcatt ctgattctgc tcgctctcta cattctgctg atcatgcgcg
    11161 ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct ggcggcttaa
    11221 tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt attctgccgg
    11281 ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt gtgctgatgg
    11341 ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg aaaagcgtgt
    11401 aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg aatgctcgcg
    11461 gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc tgcggtatgt
    11521 aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact gaacgcgacg
    11581 gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga tccgtctcga
    11641 tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa cctgacggcc
    11701 tctggcgaag ctttcgatcc gacacagttg acggcggccc atccaacgct tccgatcccc
    11761 agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg cattaatgat
    11821 cgcggcccct acggcaacga ccgcgtaatt tcgctttctc gcgcagcagc tgaccgtctt
    11881 aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca ggatggttcg
    11941 ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc cctgcctgca
    12001 cctcccgatt taagcggtgg cgcgggggca agttcagtgt ctggcccgca gggtgacatt
    12061 cttccggtca gtaactcgac gctaaaaagc gaagatccga ccggcgcgcc ggtaaccagt
    12121 agcggtttcc tcggcgctcc aacgacctta gcgcctggtg tactggaaag cagcgaaccg
    12181 acacctgcac cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac ctcgcctgca
    12241 atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt gcaagtcggg
    12301 gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca gaagttcggc
    12361 gtccccggtc gcgtaactca aaatggcgcg gtctggcgta tccagcttgg cccattcgcc
    12421 agtaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca attacagtca
    12481 tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt ctgtaaatga
    12541 cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc tatagtaggg
    12601 cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca ttttttccgc
    12661 tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta tctctgctgc
    12721 acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga tcgatgcgga
    12781 gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga acgcagatgt
    12841 ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg gccaggcaat
    12901 gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg catgggccac
    12961 cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca tgcaggttcc
    13021 ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt gcgtcgccat
    13081 ggccgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca gctacgttaa
    13141 cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg ctgatggtca
    13201 gtacagctcc gcgcgagata tggcgctgat cggccaggca ttgatccgtg acgtaccgaa
    13261 tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc agctgaaccg
    13321 taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg gacacactga
    13381 caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct tgatttctgc
    13441 ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac tgctaacctg
    13501 gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt tcgcctctga
    13561 accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag acgtgtacct
    13621 gaccattcca cgtggtcgca tgaaagatct gaaagccagc tatgtgctga acagcagtga
    13681 attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc agcttgatgg
    13741 caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag gtaacttctt
    13801 cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt aaaaattaaa
    13861 cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa ctcccgcctt
    13921 ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac caaacttaac
    13981 gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc gttacctgag
    14041 ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta caccccaacg
    14101 gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa cgccactcat
    14161 atcgagcagg ttgaaacact gtatgaagaa ctgggcaaaa tcgatattgt ccgcatggtt
    14221 ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct cctcgctgtg
    14281 atatactttt cccccacttt tactcattct ccacggagat gccgttttgt atcaggataa
    14341 aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg ctatgcatga
    14401 attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg agcactatcc
    14461 ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg gtgatattcc
    14521 ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac aacaggtgat
    14581 gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg tgaccttgct
    14641 tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc ctcgggctga
    14701 cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac gaattcgacg
    14761 cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac catttttacg
    14821 tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat ggaaacccga
    14881 agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc tactaaacaa
    14941 tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat ttacactggg
    15001 tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat attttcttta
    15061 tcacttgcag aaatgcggtg ccacgccttg ctccgcaact caccccattg attaacctat
    15121 tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata agattattgt
    15181 aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg atagtaataa
    15241 tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa tatttacaac
    15301 cttacgaaat attgatctta accttctgac tatttttgaa gctgtatatg tacataaagg
    15361 gatcgttaat gcagcgaaag tgcttaatct gaccccctcg gcaatcagtc agtctattca
    15421 gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccagg gtgtcactcc
    15481 taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt ccattcttgg
    15541 cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg cgaccactcc
    15601 ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc actatccgca
    15661 gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc agtttcaaac
    15721 cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc atgttctgtt
    15781 caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt tagaagatga
    15841 cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc aaaatttcag
    15901 cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt tcaccagcta
    15961 caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga ttattcccag
    16021 ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt ttccgtcctt
    16081 aaatgaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc gtgatcccat
    16141 cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg ttacagccag
    16201 atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt ttcgcgaccg
    16261 ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt caaaaatagt
    16321 tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc aactggaaca
    16381 cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc gatgccgata
    16441 agatggccct tatcccggtt aaaaacgtgg caacagagcg cgaagccctg ctgcgcaagc
    16501 cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc atcaaagccg
    16561 caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct aacctggcgg
    16621 aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt acccgccgtt
    16681 gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat gaaccagtga
    16741 aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc tccgttgacc
    16801 gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact gccattcggg
    16861 aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt cgtatggatc
    16921 gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac ctggaaaacg
    16981 taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg ctgaagctgc
    17041 tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtctg atggtgggac
    17101 tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt catggtgtga
    17161 cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg gttcaacgtt
    17221 acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg ggctttaccc
    17281 atgctgcatg cggtccgttt gtccgctctt cttaccacgc cgatttgcag gcgaaaggga
    17341 tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa cccgcttttt
    17401 gaagcgggtt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc cacgtcactc
    17461 tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg ccgcagcatc
    17521 gtcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc cgcccagcgt
    17581 acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacta gcagtttggt
    17641 aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga acgcgcctta
    17701 aaaaccagag aatattaacg gtcattcgac gcgtgcacac aatagcaatc tgtaacaagg
    17761 tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa gcgacggttg
    17821 tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac ttccgccata
    17881 atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc tataatccgg
    17941 ctttgaccga tatttttgtt cccgcactcc cccgccgcca ccatataaca ggtggtatcc
    18001 agcgcacgag cggcaagcaa cgttgaccaa tgatgctctt tgagcggccc gcgaacccag
    18061 gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc cagctctgga
    18121 aagcgtaagt cataacaggt catcagaccg accttcatcc cctccacctc gagtaacgga
    18181 gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc atcatagaga
    18241 tgcaatttgg cataacgggc gacgatgttt cctgcctgta gcgccaccag catattccat
    18301 gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat gttacgttta
    18361 ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga tttaaccgat
    18421 agatctgcat catgatcatc gcgcgccagc aatgcttccg gcaggacgaa cagcgatacg
    18481 tcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt ttcccacaca
    18541 gatgtaacag caaactgtcc tgcggcaacc agcattgcca cctcctgtca gagtcgtaaa
    18601 attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca aatagcagga
    18661 ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg gaagcgtggc
    18721 gagatggctg ttaagtatgc gatttaaccc gctgcatcag gcgattccgt tggggacgct
    18781 ggcagcaaac ctgattgggg cattcatcat aggaatggga ttcgcctggt tcagcaggat
    18841 gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg gcggtctaac
    18901 aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggcc gctttggctg
    18961 ggcgttactg aacgttttcg tcaaccttct ggggtctttt gccatgacag cactggcttt
    19021 ctggctgttt tcggcctcaa ccgcacacta aagaccaaaa aaaacccgct gattaagcgg
    19081 gttttgaatt cttgctgacg tatcttacag agcgattacg tttgcagcag aagggccttt
    19141 ggcaccgtta gtgatttcga actctacgcg ctgaccttca gcaagagttt taaaaccatt
    19201 agtctggatt gcagagaagt gtacgaacac gtctttgctg ccgtcttccg gagtaatgaa
    19261 accgaatcct ttggactcat taaaccactt aacgttacct ttaatcttag acatcaaaat
    19321 tacctttaca tgaaaaatag acacaaatgc tgtgtcggtt accagtacac caattgtggt
    19381 acgctttgtc cagtgcaact ttactaaaaa gtgataaaag tcgcgttatt tttgatgtta
    19441 cttattttag ctattgattt taaagaagtt actaaaactt catttgtctc aaaactgaaa
    19501 gcgcatccag gcaaagtaca cattgccatt gttgtaggta cccggaatgt aggtcatctg
    19561 aaaagtcact gggccataac ccacggaggc caatggcagt agaaccggga gagggatgta
    19621 attccagtta tcgcgtgccg ttacgccagc ggtgaatccc agacctaaat gaaaattttc
    19681 atccgccagc ggtcgccagg tactttccca tccgtatccg gcaatcggtt cccatttgtt
    19741 ccacgagtcc ctaaatgcca tggcatacag gccatgccag tttccttttt catcccaacg
    19801 cgacaggcca aaaccgccac cccacggtcg ctcgttatag cgatcggttt tttctttgtc
    19861 gtaagcgaaa cgtgcatgcc aggtgatggc aggaatatat aaatcataat gttcaggctg
    19921 ttgccaggtt tgtgcaatat tttctctaaa cgttgtcatc cactcatctg cgttagcaaa
    19981 aactttacca acgctgatta actgaataaa aacaaaggaa aagatagcga catatttact
    20041 cacgttcatt tgtgaccata aaacatttat caaaaatcta ctactagcat agcaaagcta
    20101 caattaacat aaccttaata gacccaacat aaagaataat ctgaatagct gctctattta
    20161 tttccgcttg ttcaagttaa atatttccgc gcatcgatta aagatgatgc gtcgttttct
    20221 gcgatgggaa tagtcaaaaa agaaaaacca agtctttttt gatgacaaaa tgcaatcaag
    20281 gaaaaattat tttatttttt aa
//
LOCUS       DAZGTR010000078        20299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGTR010000078 DAZGTR010000000
VERSION     DAZGTR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.078"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(497..1693)
                     /gene="hemY"
                     /locus_tag="ACHLCY_003152"
     CDS             complement(497..1693)
                     /gene="hemY"
                     /locus_tag="ACHLCY_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP0370358.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1696..2901)
                     /gene="hemX"
                     /locus_tag="ACHLCY_003153"
     CDS             complement(1696..2901)
                     /gene="hemX"
                     /locus_tag="ACHLCY_003153"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP0370359.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTTAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2923..3663)
                     /gene="hemD"
                     /locus_tag="ACHLCY_003154"
     CDS             complement(2923..3663)
                     /gene="hemD"
                     /locus_tag="ACHLCY_003154"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP0370360.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3660..4601)
                     /gene="hemC"
                     /locus_tag="ACHLCY_003155"
     CDS             complement(3660..4601)
                     /gene="hemC"
                     /locus_tag="ACHLCY_003155"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP0370361.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4988..7534
                     /gene="cyaA"
                     /locus_tag="ACHLCY_003156"
     CDS             4988..7534
                     /gene="cyaA"
                     /locus_tag="ACHLCY_003156"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP0370362.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDNDTPLLQQYFS"
     gene            complement(7574..7894)
                     /gene="cyaY"
                     /locus_tag="ACHLCY_003157"
     CDS             complement(7574..7894)
                     /gene="cyaY"
                     /locus_tag="ACHLCY_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP0370363.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7942..9267)
                     /locus_tag="ACHLCY_003158"
     CDS             complement(7942..9267)
                     /locus_tag="ACHLCY_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP0370364.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAVTDGQTLKP"
     gene            complement(9264..10772)
                     /locus_tag="ACHLCY_003159"
     CDS             complement(9264..10772)
                     /locus_tag="ACHLCY_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP0370365.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWDDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            complement(11110..11664)
                     /locus_tag="ACHLCY_003160"
     CDS             complement(11110..11664)
                     /locus_tag="ACHLCY_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP0370366.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            12014..12217
                     /gene="yifL"
                     /locus_tag="ACHLCY_003161"
     CDS             12014..12217
                     /gene="yifL"
                     /locus_tag="ACHLCY_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0370367.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12254..13078
                     /gene="dapF"
                     /locus_tag="ACHLCY_003162"
     CDS             12254..13078
                     /gene="dapF"
                     /locus_tag="ACHLCY_003162"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP0370368.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            13075..13782
                     /gene="yigA"
                     /locus_tag="ACHLCY_003163"
     CDS             13075..13782
                     /gene="yigA"
                     /locus_tag="ACHLCY_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFP0370369.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13779..14675
                     /gene="xerC"
                     /locus_tag="ACHLCY_003164"
     CDS             13779..14675
                     /gene="xerC"
                     /locus_tag="ACHLCY_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP0370370.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14675..15391
                     /gene="yigB"
                     /locus_tag="ACHLCY_003165"
     CDS             14675..15391
                     /gene="yigB"
                     /locus_tag="ACHLCY_003165"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP0370371.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15475..17637
                     /gene="uvrD"
                     /locus_tag="ACHLCY_003166"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15475..17637
                     /gene="uvrD"
                     /locus_tag="ACHLCY_003166"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP0370372.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17784..18548)
                     /gene="yigE"
                     /locus_tag="ACHLCY_003167"
     CDS             complement(17784..18548)
                     /gene="yigE"
                     /locus_tag="ACHLCY_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP0370373.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18710..18766
                     /gene="ysgD"
                     /locus_tag="ACHLCY_003168"
     CDS             18710..18766
                     /gene="ysgD"
                     /locus_tag="ACHLCY_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP0370374.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18918..19868
                     /gene="corA"
                     /locus_tag="ACHLCY_003169"
     CDS             18918..19868
                     /gene="corA"
                     /locus_tag="ACHLCY_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP0370375.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19910..>20299)
                     /locus_tag="ACHLCY_003170"
     CDS             complement(19910..>20299)
                     /locus_tag="ACHLCY_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP0370376.1"
                     /translation="DAGNSVFLIVDHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHT
                     WYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGIN
                     DFTEYQLTQENKIERVFNPHDFKKDNF"
BASE COUNT         4745 a         5221 c         5260 g         5073 t
ORIGIN      
        1 atgtaaggca ggtttattat atcgcgttga ttattgatgc tttttttagt tttaacggca
       61 attaatatat gtgttattaa ttgaatgaat tttatcattc ataataagta tgtgtaggat
      121 caagctcagg ttaaatattc actcaggaag ttattactca ggaagcaaag aggattacag
      181 aattatctca taacaagtgt taagggatgt tatttcccga ttctctgtgg cataataaac
      241 gggtagatgc tcattccatc tcttatgttc gccttagtgc ctcataaact ccggaatgac
      301 gcagagccgt ttacggtgct tatcgtccac tgacagatgt cgcttatgcc tcatcagaca
      361 ccatggacac aacgttgagt gaagcaccca cttgttgtca tacagacctg ttttaacgcc
      421 tgctccgtaa taagagcagg cgttttttta tgcatcagaa aggtcccgga ggtgcttgcc
      481 tccgggtgag aaggaactac tgtggtgggt tgttctgcaa cgttaacatc aaaccgtcgc
      541 gacgcatcgc tgcagcttct tccggcttgt gcagtctgtc cagcgcgtcg gcaagccatg
      601 cgtaatcgta ggcgtccgga cgttgtttca gcgccgcgcg gaaggcgagc gatgcttcct
      661 gccattctcc gtgcttcatc agtgactggc ccagtgtgct ccacaacagc gggcgatcgc
      721 cgacgttttt gatctgctgg cgcagcactt tttccagctg ttccggattg ttggttttca
      781 gtcgaggaat cggcagcagt aggcgatcgt cgtactggcg tttcaggcca tcgatgataa
      841 tttgctgggc agtatcatga tcgtcacatt caataagatg ttccgccatt gccacctgca
      901 acgctacctg atgacgcgtt ttccggcttt ggtttttcca ccagttacgc aaaccttcac
      961 taccgttatc ggcacgcgcc tgatccatca ggccaatcca tgcctgttgt tccagcattg
     1021 cacgatgttc ttcatcacca acatgggctt tcgccattga tgggatgata tccagcagcg
     1081 aactccatgc acctgtgcgg atatacgcct gttccgccag acgtaatact tccggatggc
     1141 gtggcgtaac ttccagcagc ttatccacgc cgtggcgtgc agcatggttt tcattacggg
     1201 ccagttgcag acgtacacgg gtgatttcta ccggaatggt gtcattgccg gccagctctg
     1261 ctgcgcgttc cagatgttgg ttggcgcgtg cttcatcacc acgttgttgc gcggcttcgg
     1321 cagccagtag atagttcacc accggttgtt ccgcgtgatc ggcatttttc gccatcagct
     1381 tttcaacttg ctgataatcg ccttccgcca gtttcagcag cgcctgttcg gtctgcttac
     1441 gggcacggcg acgcttacgt ccgacaaacc acccacgggt gtgtgcgcct gtgcggaaga
     1501 tccgccgcag tagccactca atggcaaaca gcactaccat cgccagaatc aaaataatcg
     1561 ccaggcccgt gacgctggtt tcgatattgt agttgtcagt ctggatcagc acgtaaccct
     1621 gatgaccggc aatcatcggg ccaaccacta tccccgcaat cagcaaaaca aagagtaata
     1681 acacttttag catggttatt ctccttgcgg cgcggctgcc ggagtatcac cttgcggtgc
     1741 aggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct tccgttgtcc ccgctgccgg
     1801 ttgtgccagc aggttacgca cgcgggtctg catcagtttt tccagcatcg cctggctttg
     1861 cagggtttcc ggaagatcca tcgagatatt ttgctggctt aactggtcca cctcgtcgag
     1921 gaacgctttg gtggtggcat catcagtatc gtagtaagct cgtacccagg tggagacgtt
     1981 ctccagcgcc tggcgataag tctcttcctg gtgacgcggt acagcttgtg ctgcgaccag
     2041 caggcgagag cgaatatttt cgcgcagata gatatcctga tttggcgcta acagcggtac
     2101 ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc ataaagttct gccagctttt
     2161 ttgcagattg atacgccatt cgctgatgga actggaaagc tcttcaccgt cggaatccat
     2221 cggcgaacca tcgctgtcat tatcggccag acgcaggtta tctacctgat ttgaaagctg
     2281 attaagctta aggatgatgc cgtcataatc cacctgcgaa actgcagaaa ggctggcgat
     2341 atcatcggta attgcccgac gaacggtaat cagactcggg tcattcatat ccgccaggct
     2401 ggcatctgca cttttcagca acgctgcagt ggtcgtgacg tcctgatcgc tccacagctt
     2461 ccgtccggcg agtttcacca gaaaatcggc ctgagccagc agccaggttt tagcatcgct
     2521 gccggaaatg gtggcgacct tttgttggac ttcatccaac tgttttgcca gcgtttcttg
     2581 ctgacgattc gcctgcttaa gctgtgcagc ttgttgctta ataatgcctt ccagctcggc
     2641 tttttggctc tcctgggctt tttgcaatgc cgtcagttgg ttagccaggg catcgctggt
     2701 ggcggtctga ttgacggcct gttgtttacc ccagccatac aaaccgacac ccgccgccag
     2761 agcaatagcg atagccaccg cgctgagaat caatgcagta ttgttcttac tctttttttc
     2821 tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg gtctcttcaa ccacggcgga
     2881 ggttttttct tgttccgtca ttatggcttc ctgttatgag agttattgta atgcccgtaa
     2941 aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct tgccagccca gttcccgggc
     3001 gagtttcgcc aaacgctcac tgacgaccaa tagtcgacag tgtagtaacc agtgctcacg
     3061 ataccattgt gggatcagcg accagagttg ctgcaacatt tcaccgctgg taacaacgac
     3121 catcgtcacc tcgcgggatt gccagcgcat cgcttcttct gcaccatcgt aatggattgc
     3181 gcatcgttga taacattcac aaaaagtgac ctcagcaccg cgcgctgtca gggtatcccc
     3241 aattagctca cgaccgccat tgccacgtaa tatcagcgca cgtttgcccg caatattttg
     3301 taattcaggt aattgtagca agacttcgct gatttcccga tcctgcgggt agagaatctt
     3361 ctgtccgctt acggtatgta gcgccagtgc ggtggtgcgt ccaatggcga aataatcagg
     3421 tagtcggggc catttacgat cttgctgatg cagctgtgat tgggcaaaag caaccgcgtg
     3481 ttgcgagagg gcaaacaaca gatcgctctc ccccagcgcc gccagttgat cagcaagttg
     3541 cggtaattgt cgacccggag aaaactcaat cagcggaaaa tgccaggcca cctgccccag
     3601 tgtgcgcaga cggctcacta actcttctcc agcgggagac gggcgggtga ccaagatact
     3661 catgccgggg cgtctccgtt atagacttca gcgaggatct cgcgcgcgcc gttattcagt
     3721 agctcttctg ccagcgaaat ccccatttgt tcggcatttt gcggcgcacc gcggcgttca
     3781 ccgcgaataa tctgcgaacc gtccggcgcg ccgaccagcg cacgcagcca gatttcacca
     3841 tcaataagct cggcgtagct accaattggc acctgacatc caccttcgag acgggtattc
     3901 atggcgcgtt ctgcggtaac gcgcagtgca gtttcgtggt gattcagcgc ggcaagcagc
     3961 tcgcgagtgc gtgtatcatc aaggcggcat tcaataccga ctgcaccttg tcctaccgcc
     4021 ggaagagaaa tctcgggtgg taacgcggcg cgaatgcggg actccagacc taaacgtttt
     4081 agtccggcta cggcaagaat gatggcatcg tattcgccgt tatctagttt gctcaggcga
     4141 gtgccgacgt tgccgcgcag ggagcggata atcagatccg ggcggcgttc agccagttgg
     4201 cactggcgac gtaaactgga cgtcccgacg atactgcctg ccggtaacgc atccagactg
     4261 tcataattat tggacacaaa ggcatcgcga ggatcttcac gctcacaaat agtgaccaac
     4321 cccagacctt gcgggaattc aaccggcaca tctttcattg aatgtacggc gatatcggcg
     4381 cgattctcca ggagcgcgac ttccagctct tttacaaata agccttttcc gcctactttc
     4441 gccagcggcg tatcaagaat cacatcgccg cgcgtcacca tcggtaccag ttcaaccacc
     4501 aggcccggat ggctcgccat caacttgtct ttgacatagt gtgcctgcca gagtgcaagt
     4561 gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca tgcttgttac cgtcattatc
     4621 atccgtggtc catcctaaca tccttgccag agtgatgtca gtgttgtggt gaaacgtaga
     4681 cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc tgacaggcgt ttcacgccgt
     4741 cgtaataagg aatttacaga gaataaacgg tgctacactt gtatgtagcg catctttctt
     4801 tacggtcaat cagcaaggtg ttaaattgat cacgttttag accatttttt cgtcgtgaaa
     4861 ctaaaaaaac caggcacgaa aagtggtaac ggttaccttt gacatacgaa atatcccgaa
     4921 tgccgcgtgt taccgttgat gttggcggaa tcacagtcat gacgggtagc aaatcaggcg
     4981 atacgtcttg tacctctata ttgagactct gaaacagaga ctggatgcca taaatcaatt
     5041 gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc caacaggtct acagtctact
     5101 gccgacattg ttgcactatc accatccgct aatgccgggt taccttgatg gtaacgttcc
     5161 caaaggcatt tgcctttaca cgcctgatga aactcaacgc cactacctga acgagcttga
     5221 actgtatcgt ggaatgtcag tacaggaccc accgaaaggt gagcttccaa ttactggtgt
     5281 atacaccatg ggcagcacct cgtccgtagg gcaaagttgt tcctctgacc tggatatctg
     5341 ggtctgtcat caatcctggc tcgatagcga agagcgccag ttactacaac gtaaatgtag
     5401 tctgctggaa agctgggccg cctcgctggg tgtggaagtc agcttcttcc tgattgatga
     5461 aaaccgcttc cgtcataatg aaagcggcag cctggggggc gaagattgtg gctccaccca
     5521 gcatatactg ctgcttgacg aattttatcg taccgccgtg cgtctcgccg gtaagcgtat
     5581 tctgtggaat atggtgccgt gcgacgaaga agagcattac gacgactatg tgatgacgct
     5641 ttacgcgcag ggcgtgctga cgccaaatga atggctggat ctcggtggct taagctcgct
     5701 ttctgctgaa gagtactttg gtgccagcct ttggcagctc tacaagagta tcgattcccc
     5761 atacaaagcg gtactgaaaa cactgctgct ggaagcctat tcctgggaat acccgaaccc
     5821 acgtctgctg gcgaaagata tcaaacagcg tttgcacgac ggcgagattg tatcgtttgg
     5881 tctcgatcca tactgcatga tgctggagcg tgttactgaa tacctgacgg cgattgaaga
     5941 ttttacccgt ctggatttag tacgtcgctg cttctattta aaagtgtgcg aaaagctcag
     6001 ccgtgaacgc gcctgcgtag gctggcgtcg cgcagtgttg agccagttag tgagcgagtg
     6061 gggttgggac gaagctcgtc tggcaatgct cgataaccgc gctaactgga agattgatca
     6121 ggtgcgtgag gcgcacaacg agttgctcga cgcgatgatg cagagctacc gtaatctgat
     6181 ccgctttgcg cgtcgcaata accttagcgt ctccgccagt ccgcaggata tcggcgtgct
     6241 gacgcgtaag ctgtatgccg cgtttgaagc attaccaggt aaagtgacgc tggtaaaccc
     6301 gcagatttca cccgatctct cggaaccgaa tctgaccttt atttatgtgc cgccgggccg
     6361 tgctaaccgt tcaggttggt atctgtataa ccgcgcgcca aatattgagt cgatcatcag
     6421 ccatcagccg ctggaatata accgttacct gaacaaactg gtggcgtggg catggtttaa
     6481 cggcctgctg acctcgcgca cccgtttgta tattaaaggt aacggcattg tcgatttgcc
     6541 taagttgcag gagatggtcg ccgacgtgtc gcaccatttc ccgctgcgct tacctgcacc
     6601 gacaccgaag gcgctctaca gcccgtgtga gatccgccat ctggcgatta tcgttaacct
     6661 ggaatatgac ccgacagcgg cgttccgcaa tcaggtggtg cattttgatt tccgtaagct
     6721 ggatgtcttc agctttggcg agaatcaaaa ttgcctggta ggtagcgttg acctgctgta
     6781 ccgcaactcg tggaacgaag tgcgtacgct gcacttcaac ggcgagcaat cgatgatcga
     6841 agccctgaaa actattctcg gcaaaatgca tcaggacgcc gcaccgccag atagcgtgga
     6901 agtcttctgt tatagccagc atctgcgcgg gttaatccgt actcgcgtgc agcaactggt
     6961 ttctgagtgt attgaactgc gcctttccag cacccgccag gaaaccgggc gtttcaaggc
     7021 gctgcgcgtt tctggtcaaa cctgggggtt gttcttcgaa cgcctgaatg tatcggtaca
     7081 aaaactggaa aacgccatcg agttttatgg cgctatttcg cataacaaac tgcacggctt
     7141 gtcagtgcag gttgaaacca atcacgtcaa attaccggcg gtggtggacg gctttgccag
     7201 cgaagggatc atccagttct tcttcgaaga aacgcaagac gagaatggct ttaatatcta
     7261 cattctcgac gaaagcaacc gggttgaggt gtatcaccac tgcgaaggca gcaaagagga
     7321 gctggtacgt gacgtcagtc gcttctactc gtcatcgcac gatcgcttca cctacggctc
     7381 aagctttatc aacttcaacc tgccgcagtt ctatcagatt gtgaaggttg atggtcgtga
     7441 acaggtgatt ccgttccgca caaaatctat cggtaacatg ccgcccgcca atcaggacaa
     7501 cgatacgccg ctattacagc agtatttttc gtgatgagcg tgccggaaag cgaggcttat
     7561 ccggcatgca atcttagcgg aaactgactg tttcacccgc ttgctgtgtc gccgcctgtt
     7621 ccagcaaatc ccagaaggtt tcgccgctgc gatcgcaaat ccattcatcg ccttttaggt
     7681 caaaatggta gccgccctgt ttggttgcca gccacacctg gtgcagcggc tcctggcggt
     7741 taataatgat tttgctgccg ttttcaaagg taatggtcag tacgccgccg ttgatttcgc
     7801 agtcgatatc gctgtcgcca tcccagtcgt ccaggcgttc ttcaatggtc agccagagtt
     7861 gatcagccag gcgatgaaat tcactgtcgt tcattgttgt atcctgtctt taagtgatgg
     7921 cggcagtata gcggtatggg gtcagggctt caaagtttgc ccgtctgtta ctgcgttccg
     7981 gcatgagtca tccgtcaacg gaataataat gtctctgttg tgaagttcac gcacattctc
     8041 ttctctgacg tgtgttttcg ccgggcaggg ggagggcgta atccttacct ctgtatcggt
     8101 ccatgtataa acattgcgat cgtcataagc gaagcgatcg tttttagtta gcggaccgtt
     8161 aactggaata atttgcacgc tctttgaccg taattggcgg ataaaataag catgctgttt
     8221 gccgctatta aatgagcgat caatgaccag gtctggcgta tccagcggga ttatctcaag
     8281 ctggtcttca ccaaatgtcg ataattttgt tacatacagc cgatctttat attgcgcaat
     8341 attgccagta atcagatgga attgttcagg ttcaatatct ggtaggtcta tccacttgct
     8401 ccactgacgg tcggacgtga gctgtttacg ccagcgaacg cgactttccc ctacttcaaa
     8461 agagacgtca tcatctacgg gtatcgcttt gccagacaat tgcccaaagg gataacgatg
     8521 cataccctta ttgtcgcgaa aaaccagcag tgaatgaggt atccagcgaa ttatctcaaa
     8581 tgagtctgca ttcgccgtaa ggtgaacgcc atttaaataa atattctgcc cactgcggat
     8641 aagcagatca ttggaataca aatttctgtt tacactcata agacgtggat taatacccca
     8701 ccatcttttt tctaatacag taacattact gctgctcccc tgacgtcgac cggacaaata
     8761 gagattttta ctatccacca gcgtggtaga gttaccttcg atagctttta gtgtcgccag
     8821 atcaacacgt gacgcgccgc tattactttc ggtgcgctgt ccgtcaaaat agaggctata
     8881 tttatcgacc gcgaagcggc ccatagcctg aaagctggca atatcgacag tgggtgttcc
     8941 cggtggattg ctaacgattt tgccacgcca gagaataacg cggccgtctg tcacccagtg
     9001 gtattcctca ttttttatgc ttatttcatc ggtgggtata tagccttcca ccaactcccg
     9061 ctccccggat atatttgtca caaggcgcaa atcggccctg ctcatctccg gaaactgggc
     9121 aatgattttc ttatcccccc aataaccttt accgtaagac ataacaccgt gatcattgta
     9181 atagtcgaac cctatggcct gtacagcagg cgtcagaaat gtgtgtccca acaggatgag
     9241 tgcaactgtg attatttgac ccttcatttt gtcgcctcat tgcgcggggc aggaataaat
     9301 tcgccgtcat cagtaatttt tccgtccaga tatcccgttt tgtaatttac gccatagcca
     9361 tatcccgaat gttgttcctt ccagattaag cgaacttgtt tcggatcagc ggtgcggtat
     9421 cgccagagct gcgcgccatc aaagctgtaa atgtagcggt tatcgcgggc ataccatctg
     9481 cgcaaaaagg gatggtcatt aagatgggct tcgcgttcat ccggagaacg gttatccagt
     9541 aagatcagcg gtccgtcaga ttcaaacaca tgtaaaccgg aatggtatgg ccacccttcg
     9601 gcacgcgtaa gtaaatagcc gtggcgtttc ccggcgttga aacgtttgtt gataataaga
     9661 ttgggatcat caagtttcac gatttccagc ttgcggtcac cagactctgt ttcgatgatg
     9721 gtataaagcg agtcctgata ctgggcgaca gcatcactga tgggttgaaa ctgctccgga
     9781 tcgagtccgg gtaattcttc ctgtttacaa gcaggtcctt tacgccagaa cactttcttt
     9841 tccagcagag aaaaatccag acagtgcttt gttaaatctt catcccactc cagattttcc
     9901 ttgtcgagga ctttacgctg cagcccgttt ttatcacgcc aggtcaagag tgagccgggc
     9961 atccagcgca cgacggaaaa ggtgtccggg tcggcatcaa gctgttcgcc gttgatcagg
    10021 attttggtcc gtgtacgagc cagggtatcc caggggccaa atggcatagc cacacaggga
    10081 ttaaacgcgg tagaaaactt tcctcgctga tcccatgact tttgtgccag cacacggaaa
    10141 ctatcgggat ctgcgaggcg atgcccattg atatacaaga aattagcgtc acgtaatatc
    10201 agccccagta agtcaggatc ccagggaggc cggaattcca cctgatgcag tgttttgata
    10261 tctaagcgat ttcccccgcc attgttatcg gtgcgcttgc cttcgaaata gagactgtgt
    10321 ttatctgcgg caaaatcatc ccacgccttg aacgaggcga catcgacggg aggtttgtct
    10381 ggcgggtttt gcacaacttc acccgcgtag aggatatagc gaccatcgct atgccaggag
    10441 taatgaggat accaggcctg tgcgccttca tagaggtagt cggttgtcag atcagtcaga
    10501 ggaggtactt tatcccattt gtctagtgtt gtcgggtcta ttaataaatt gggtaaacgg
    10561 cgcaaacttt tgtaattcac gtcaggaaga gaaagatttg tttctttata agggcgcagg
    10621 tataccacgc cttttccctg tttctggtaa atattatgtt caggcggatc aagccgacat
    10681 ccccatgccg acgataaaaa caatgaacaa gacaacaaac aaaccacatt gcgatagtgc
    10741 ataaagccat cctggcgcga ggtgccgatc acgaaactac cagcagaaca taattcctcg
    10801 cacgtaagcg taacttttgc ggcaaatcgt cacttttcag aatctataaa ggccatcgct
    10861 attttctgat agttgctggg gtaaacttga ggttttcatt cacgatataa atacgagcta
    10921 ctgcgccatg gtgtgactga tcacgttaaa atgtgccgga ttgacgccgg gaagggtgtc
    10981 aacatgcgac tctgcccacc gttacctact ttattgtctt tcgcgtgttg tacttaggtc
    11041 ccaccaccgg tatctaacat tccccacatt attacgcttt ctgctgaacg tgatttttat
    11101 agaaatctat caattcattt tccctgtacc ataaagttta tctttcacat cctgccgaac
    11161 gcgggtcttg taccccccgt tgtgaagatc ttcatacgga gaaaaagaat ctttactcca
    11221 ccccagcagc actttatcgc caggcataaa atgcacatgc agaaaatctt ctccccattc
    11281 tcttttgggg agaggtaacg tcatattccg tttctccaga cgcattccag cattatactg
    11341 tgtacgagta gtacttaatg tccagataac ctctgccgta tccccactaa catcaccaca
    11401 acaggttgtt gaaccgccac catttgcaaa agcattcgcg cccgccacgc cgttaacact
    11461 gaatcccagt atcgggcggt caatttcact gtggatttcc agaattactc cacctcttgg
    11521 tcctccccat actgcctgtc ctatattcca caacacgacc agtaccggaa cgacaattaa
    11581 cgtccgccat acccagcgcc cccaccgggc ataaccgcgc gtaagctttt ggtcaaattt
    11641 ttccagttgg gtaaatagcc ccatcagatt ttattccctc ttatcaccag gttggcaaag
    11701 ttgtatgtca ctggcgacta aggtcagaca acaaaattgc atggtgcgcc actcttttaa
    11761 aagctccatg ccgacgataa gaacaacgaa caagccaaca aacaaactac attgcgatag
    11821 tgcatagagc catcctggcg cgaggtgccg atcacgaaac taccagcaaa acataaatcc
    11881 ccacgagtaa gcgttatact cgcagcattt cctcactttt cagacttcat aaagagtcgc
    11941 taaacgcttg cttttacgtc ttctcctgcg atgatagaaa gcagaaagcg atgaacttta
    12001 caggcaatcc ataatgaaaa acgtgtttaa ggcactcact gtattaatta ctctcttcag
    12061 cctgacgggc tgcggtctga aaggtccgct ctatttcccg cctgcagata aaaacgcacc
    12121 gccgccgacc aaaccggtag agacgcaaac gcaatccacg gtgccggata aaaacgatcg
    12181 cgccactggc gatggtccat cccaggtgaa ttactaaaag tcagtttctg tacccgcgtg
    12241 attggagtaa atgatgcagt tctcgaaaat gcatggcctt ggcaacgatt ttatggtcgt
    12301 cgacgcggta acgcagaatg tctttttttc accggagctg attcgtcgcc tggctgatcg
    12361 gcacctgggg gtagggtttg accaactgct ggtggttgag ccgccgtatg atcctgaact
    12421 ggattttcac tatcgcattt tcaatgctga tggcagtgaa gtggcgcagt gcggcaacgg
    12481 tgcgcgctgc tttgcccgtt ttgtgcgtct gaaaggactg accaataagc gtgatatccg
    12541 cgtcagcacc gccaacgggc ggatggttct gaccgtcacc gatgatgatc tggtccgcgt
    12601 aaatatgggc gaacccaact tcgaaccttc cgccgtgccg tttcgcgcta acaaagcgga
    12661 aaagacctat attatgcgcg ccgccgagca gacaatctta tgcggcgtgg tgtcgatggg
    12721 aaatccgcat tgcgtgattc aggtcgatga tgtcgatacc gcggcggtag aaacgcttgg
    12781 tcctgttctg gaaagccacg agcgttttcc ggagcgcgcc aatatcggtt ttatgcaagt
    12841 ggttaagcgc gagcatattc gtttacgcgt ttatgagcgt ggggcaggag aaacccaggc
    12901 ctgcggcagc ggcgcgtgtg cggcggttgc agtagggatt cagcaaggtt tgctggccga
    12961 agaagtacgc gtggaactcc ccggcggtcg tcttgatatc gcctggaaag gtccgggtca
    13021 cccgttatat atgactggcc cggcggtaca tgtctacgac ggatttattc atctatgaag
    13081 caaccagggg aagaactgca ggaaacactc acggagcttg atgaccgggc ggttgtcgat
    13141 tatctgatta aaaatcctga gttttttatc cgtaatgcgc gcgcagtaga agcgatacgt
    13201 gtgccgcatc cggtacgcgg caccgtttcg ttggtcgagt ggcacatggc ccgcgcacgt
    13261 aatcatattc atgttctgga agagaacatg gcgctgttga tggaacaggc tatcgccaac
    13321 gaaggcctgt tttatcgcct actctacctg cagcgcagtc tcaccgccgc cagcagtctc
    13381 gacgatatgc tgatgcgctt tcaccgctgg gcgcgcgatc tcggcctggc aggtgcgagt
    13441 ctgcgcctgt ttccggatcg ctggcgctta ggtgcgccgt cgaaccacac tcatctggca
    13501 ttaagccgtc agtctttcga accgctgcgt attcagcgtt tggggcagga acagcactat
    13561 cttgggccgc ttaacggacc agagctgctg gtggtgctac cggaagcgaa agcggtggga
    13621 tcggtggcga tgtcgatgct gggaagcgat gctgatttgg gtgtcgtgct gtttaccagt
    13681 cgcgatgcca gtcactatca acaagggcaa ggaacgcagt tacttcatga aattgcgctg
    13741 atgttgccgg agcttctgga gcgttggatt gaacgcgtat gaccgattta cacaccgatg
    13801 tagaacgcta cctacgttat ctgagcgtgg agcgccagct tagcccgata accctgctta
    13861 actaccagcg tcagcttgag gcgatcatca attttgccag cgaaaacggc ctgcaaagct
    13921 ggcagcaatg tgatgtgacg atggtgcgca attttgctgt acgcagtcgc cgtaaagggc
    13981 tgggagcagc aagtctggcg ttacggcttt ctgcgctacg tagctttttt gactggctgg
    14041 tcagccagaa cgaactcaaa gctaacccgg cgaaaggtgt ttcggcaccg aaagcgccgc
    14101 gtcatctgcc gaaaaacatc gacgtcgacg atatgaatcg gctgctggat attgatatca
    14161 acgatcccct cgctgtacgc gaccgtgcaa tgctggaagt gatgtacggc gcgggtctgc
    14221 gtctttctga gctggtgggg ctggatatta aacacctcga cctggagtct ggtgaagtgt
    14281 gggttatggg gaaaggcagc aaagagcgcc gcctgccgat tggtcgcaac gctgtggcgt
    14341 ggattgagca ctggcttgat ttgcgcgacc tgtttggtag cgaagacgac gcgctttttc
    14401 tgtcgaaact gggcaagcgt atctccgcgc gtaatgtgca gaaacgcttt gccgaatggg
    14461 gcataaaaca agggctgaat aatcacgttc acccacataa attacgtcac tcgttcgcta
    14521 cgcatatgct ggagtcgagc ggcgatcttc gtggtgtgca ggagctactg ggtcatgcca
    14581 acctctccac cacgcaaatc tatactcatc ttgattttca acaccttgcc tcggtgtacg
    14641 atgcggcgca tccacgcgcc aaacggggga aataatgcgt ttttaccggc ctttggggcg
    14701 catctcggcg ctcacctttg acctggatga taccctttac gataaccgtc cggtgatttt
    14761 gcgcaccgag cgagaggcgc ttacctttgt gcaaaattat catccggcgc tgcgcagctt
    14821 ccagaatgaa gatctgcaac gcctgcgcca ggcggtacgg gaagcggaac ccgagattta
    14881 tcacgacgtg acgcgctggc gttttcgttc gattgaacaa gcgatgctcg acgccgggct
    14941 gagtgccgaa gaagccagtg caggcgcaca cgcagcaatg atcaactttg ccaaatggcg
    15001 cagccgaatc gacgtcccgc agcaaactca cgacacctta aaacagctgg cgaagaaatg
    15061 gccgctggtg gcgatcacca acggtaacgc ccagccggaa ctgtttggtt tgggggatta
    15121 ttttgagttt gtgctgcgcg ctggcccgca cgggcgctca aaaccgttca gcgatatgta
    15181 ctttctggct gcggaaaaac tcaacgtgcc gatcggcgag atcttacatg ttggggacga
    15241 tctcaccact gacgtgggtg gggcaattcg cagcggaatg caggcctgtt ggatcagacc
    15301 ggaaaatggc gatctgatgc aaacctggga cagccgttta ctgccgcatc tggaaatttc
    15361 ccggttggca tctctgacct cgctgatata atcagcaaat ctgtatatat acccagcttt
    15421 ttggcggagg gcgttgcgct tctccgccca acctattttt acgcggcggt gccaatggac
    15481 gtttcttacc tgctcgacag ccttaatgac aaacagcgcg aagcggtggc cgcgccacgc
    15541 agcaaccttc tggtgctggc gggcgcgggc agtggtaaga cgcgcgtact ggtgcatcgt
    15601 atcgcctggc tgatgagcgt ggaaaactgc tcgccatact cgattatggc ggtgacgttt
    15661 accaacaaag cggcggcgga gatgcgtcat cgtatcgggc agctgatggg caccagccag
    15721 ggcggcatgt gggtcggcac cttccacggg ctggcgcacc gtctgctgcg tgcgcaccat
    15781 atggacgcca atctgccgca ggattttcag atcctcgaca gcgaggacca gctacgcctg
    15841 ctcaaacgcc taatcaaggc gatgaacctc gacgagaagc agtggccgcc gcgccaggca
    15901 atgtggtaca tcaacagcca gaaagatgaa ggcctgcgtc cacatcatat tcaaagctac
    15961 ggtaatccgg tggagcagac ctggcagaag gtgtatcagg cgtatcagga agcgtgtgac
    16021 cgcgcgggcc tggtggactt cgccgagctg ctgctacgtg ctcacgagtt gtggcttaac
    16081 aagccgcata tccttcagca ctatcgcgaa cggtttacca atatcctggt ggacgaattc
    16141 caggatacca acaacattca gtatgcgtgg atccgcctgc tggcgggcga taccggcaaa
    16201 gtgatgatcg ttggcgatga cgatcagtca atctacggct ggcgtggagc gcaggtggag
    16261 aatattcagc gtttcctcaa tgatttcccc ggtgctgaaa ctatccgtct ggagcagaac
    16321 taccgctcta ccagcaatat tctgagcgcc gcgaacgccc tgattgaaaa caacaacggg
    16381 cgtttgggta aaaaactgtg gaccgatggc gcggacggtg agcctatttc cctctattgc
    16441 gcttttaacg aactcgatga agcgcgtttt gtggttaacc gcatcaaaac ctggcaggac
    16501 aacggcgggg cgcttgccga gtgcgccatt ctctaccgca gtaacgccca gtcgcgtgtg
    16561 ctggaagaag cactgttgca ggcgagtatg ccgtaccgta tttacggcgg gatgcgcttc
    16621 ttcgaacgcc aggaaatcaa agatgcgctc tcgtatctgc gcctgattgc caaccgtaac
    16681 gacgacgcgg cctttgagcg tgtggtgaat acgccaacgc ggggtattgg tgaccggacg
    16741 ctggacgtgg tacgtcagac atcgcgcgat cgccagttaa cactctggca ggcatgtcgt
    16801 gaactgttgc aggaaaaagc cctcgccgga cgtgctgcca gcgccttaca gcggtttatg
    16861 gaactgatcg acgccttagc gcaggaaact gccgatatgc cgctgcatgt acagactgac
    16921 cgggtaatta aagactccgg cctgcgtacc atgtacgagc aggagaaggg cgaaaaaggt
    16981 cagacgcgta tcgaaaactt agaggaactg gtgacggcaa cgcgccagtt cagctacaac
    17041 gaagaagacg aagatttaat gccgctgcag gcgttcctat cccatgcggc gctggaagca
    17101 ggcgaagggc aggcggatac ctggcaagat gcggtacaac tgatgaccat gcactcggcg
    17161 aaaggtctgg agttcccgca ggtgtttatc gtcggtatgg aagagggcat gttcccaagc
    17221 cagatgtcgc tggatgaagg cgggcgtctg gaagaagaac gccgtctggc ctacgttggc
    17281 gtaacccgcg cgatgcagaa actgacgctg acctacgcgg aaactcgccg tctgtatggc
    17341 aaagaggttt accatcgccc gtcgcgcttt attggcgagc tgccggaaga gtgtgtggaa
    17401 gaggtgcgcc tgcgcgccac ggtaagccgc ccggtcagcc atcagcggat gggtacgccg
    17461 atggtcgaga acgacagcgg ctacaagctc ggccagcgcg tacgccacgc taagtttggt
    17521 gaaggcacca ttgtcaatat ggaaggcagc ggtgagcata gccgtttgca ggtggcattt
    17581 cagggccagg gtattaaatg gctggtggcg gcttatgccc ggctggagtc ggtgtaacgt
    17641 tgccggatgc ggtgctgcgc accttatttg gcctaaaaaa tcattcagat tcaataaatt
    17701 gcaacgtcat gtaggccgga tagggcgttt acgccgcatc cggcatctgc gccatcttca
    17761 gtatctgaca caaaactatc gttttaacct ttccgctcta cggaaatcat ggtgacaaaa
    17821 ggatagcgtt gccacgggat tgccccgcct ttcatataca tatgtgaaat cgtgccatca
    17881 agataaagca gctgctcaac gttcagtttc gctttggcat aacaggcaaa atcataaaaa
    17941 tttgtcgcct gctggctcaa caaaaacacg gcattcccat gtttattaat cccaacaccg
    18001 ttacgaattt tacgtgaggc gacgttggga tgaatacgcg gattaattac actgttttcc
    18061 atcaacattg gccctgactg caccgcaaac tgaatctctt tactggtttt gaaggcatcc
    18121 agacgaacga tgccgacttt atctcccgcg acataaaaca cgccgccagg acggataaag
    18181 aaattccctt cacctgaagc gagatttaac gccaccttct gctgaccgtt ttcgatgtac
    18241 aaaccgagcg gcgcatagct ttcatcatag atgccgccgt tcatcgccat ctgcacctga
    18301 ccctgactat tcatatccgc cagcagagca tgtaaagttc cccacgcttc gccattggct
    18361 ttttgccagt acattttcac ccgctctgtt tgaggattaa cggtatacgc ctgtacggtc
    18421 agcgtcggat ctgagagtgc gcaatcatca gcggcaacag caaacaaggg aagtaaggtg
    18481 agggtgagaa aaatccgttt gagattcaag gtgatcattc ctttaccaat gagtagccga
    18541 tgcgccatta taggtcctgg atgttggatt tttttatcct gttagcgacc ttgacgagta
    18601 ccaaaaagcg cgaagttcaa ctattgttct gtggtgttct gttgcgtgtt gacggcaaaa
    18661 ttttgctggc gtaacatgcg cgcacgatca ctctaagagg acattcgcct tggacacacc
    18721 cagtagatac tggctcacta tcctgtcatc caggatcaac tcctaaggct atcccttttt
    18781 gctgatagcc ttagcggttg tcagcgacct caatttttcc cgtcgcgctg agtcaggctg
    18841 tttaatggtc tgaaacccaa tttgtttctg tgtgcccacc gaactgtccg atattttaag
    18901 cattgggagt cccggtcatg ctgagcgcat ttcaactgga aaataaccga ctgacccggc
    18961 tggaagtcga agagtcacaa ccccttgtaa atgcagtatg gattgatctt gtcgaaccgg
    19021 acgacgacga gcgactgcgc gtacaatctg aacttggcca gagcctggca acccgcccgg
    19081 aactggaaga catcgaagca tcggcacgtt tctttgaaga cgacgacggc ctgcatattc
    19141 actccttctt cttctttgaa gatgcggaag atcacgccgg taactccact gtggcattta
    19201 ccatccgtga tggtcgtctg tttactctgc gtgagcgtga actgcctgct tttcgtctgt
    19261 atcgtatgcg tgcccgtagc cagtcgatgg tagacggtaa cgcctacgag ttgctgctgg
    19321 atctgttcga aaccaaaatc gaacagttgg cagatgaaat tgaaaatatc tatagcgacc
    19381 tggagcagtt gagccgggtg attatggaag ggcatcaggg cgatgagtac gacgaggcgc
    19441 tctccacgct ggcggaactg gaagatatcg gctggaaagt tcgcctgtgt ctgatggata
    19501 cccagcgcgc gctcaacttc ctggtacgca aggcgcgttt accgggtggg caactggagc
    19561 aggcgcgtga aatcctgcga gatatcgaat ccctgctgcc gcataacgaa tccctgttcc
    19621 agaaggtgaa cttcctgatg caggcggcga tgggctttat caacatcgag cagaaccgca
    19681 tcatcaaaat cttctcggtg gtatccgtgg tgttcctgcc accgacgctg gtggcttcca
    19741 gctacgggat gaactttgaa tttatgccag agttgaagtg gagtttcggc tacccaggcg
    19801 cgattatctt tatgatcctc gcgggcctgg caccgtatct gtactttaag cggaagaact
    19861 ggttgtaata tgagtgccgg ataaagctat ttttttatcc ggcttatttc taaaaattgt
    19921 cttttttaaa gtcatgcgga ttgaaaactc gctctatttt attttcttgt gttaattgat
    19981 actcagtaaa atcgttaata ccaatatgag tgttacgata agggtattca ttaataacca
    20041 ttaagtaatt tttcacgatg caattatcat tgctcttaat tgtcgggaaa cagtagagat
    20101 cttcatacgg cccttcgata tattttttat agtcagtgaa gccattaccg atatcaatga
    20161 cataccaggt atgccaacta ccttctggta ggggatgttg atttttaatt tcattctgat
    20221 gcttcagata ccaatttatt ttatcgctgt cagtccatgg cagatgatca acgatcagaa
    20281 agactgaatt gccagcatc
//
LOCUS       DAZGTR010000079        20193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGTR010000079 DAZGTR010000000
VERSION     DAZGTR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.079"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..184)
                     /locus_tag="ACHLCY_003171"
     CDS             complement(<1..184)
                     /locus_tag="ACHLCY_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP0370377.1"
                     /translation="MPIINKSASNYVEYISKNNPPYLSKKRDASINLNGKVSDCNGEI
                     IWCRHIASYWSEFFCSN"
     gene            complement(175..738)
                     /locus_tag="ACHLCY_003172"
                     /pseudo
     CDS             complement(175..738)
                     /locus_tag="ACHLCY_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(1069..1944)
                     /locus_tag="ACHLCY_003173"
                     /pseudo
     CDS             complement(1069..1944)
                     /locus_tag="ACHLCY_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            1952..2377
                     /gene="tnpA"
                     /locus_tag="ACHLCY_003174"
                     /pseudo
     CDS             1952..2377
                     /gene="tnpA"
                     /locus_tag="ACHLCY_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(2441..2734)
                     /locus_tag="ACHLCY_003175"
                     /pseudo
     CDS             complement(2441..2734)
                     /locus_tag="ACHLCY_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            3006..3800
                     /gene="yjfZ"
                     /locus_tag="ACHLCY_003176"
     CDS             3006..3800
                     /gene="yjfZ"
                     /locus_tag="ACHLCY_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0370378.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHTQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(3871..4320)
                     /gene="rplI"
                     /locus_tag="ACHLCY_003177"
     CDS             complement(3871..4320)
                     /gene="rplI"
                     /locus_tag="ACHLCY_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP0370379.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(4362..4589)
                     /gene="rpsR"
                     /locus_tag="ACHLCY_003178"
     CDS             complement(4362..4589)
                     /gene="rpsR"
                     /locus_tag="ACHLCY_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP0370380.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(4594..4908)
                     /gene="priB"
                     /locus_tag="ACHLCY_003179"
     CDS             complement(4594..4908)
                     /gene="priB"
                     /locus_tag="ACHLCY_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP0370381.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(4915..5310)
                     /gene="rpsF"
                     /locus_tag="ACHLCY_003180"
     CDS             complement(4915..5310)
                     /gene="rpsF"
                     /locus_tag="ACHLCY_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP0370382.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5637..5912
                     /gene="yjfY"
                     /locus_tag="ACHLCY_003181"
     CDS             5637..5912
                     /gene="yjfY"
                     /locus_tag="ACHLCY_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP0370383.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(6041..6727)
                     /gene="ulaF"
                     /locus_tag="ACHLCY_003182"
     CDS             complement(6041..6727)
                     /gene="ulaF"
                     /locus_tag="ACHLCY_003182"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP0370384.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(6727..7581)
                     /gene="ulaE"
                     /locus_tag="ACHLCY_003183"
     CDS             complement(6727..7581)
                     /gene="ulaE"
                     /locus_tag="ACHLCY_003183"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP0370385.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(7591..8241)
                     /gene="ulaD"
                     /locus_tag="ACHLCY_003184"
     CDS             complement(7591..8241)
                     /gene="ulaD"
                     /locus_tag="ACHLCY_003184"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0370386.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(8255..8719)
                     /gene="ulaC"
                     /locus_tag="ACHLCY_003185"
     CDS             complement(8255..8719)
                     /gene="ulaC"
                     /locus_tag="ACHLCY_003185"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP0370387.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(8729..9034)
                     /gene="ulaB"
                     /locus_tag="ACHLCY_003186"
     CDS             complement(8729..9034)
                     /gene="ulaB"
                     /locus_tag="ACHLCY_003186"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP0370388.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(9050..10447)
                     /gene="ulaA"
                     /locus_tag="ACHLCY_003187"
     CDS             complement(9050..10447)
                     /gene="ulaA"
                     /locus_tag="ACHLCY_003187"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0370389.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            10802..11866
                     /gene="ulaG"
                     /locus_tag="ACHLCY_003188"
     CDS             10802..11866
                     /gene="ulaG"
                     /locus_tag="ACHLCY_003188"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP0370390.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            11974..12729
                     /gene="ulaR"
                     /locus_tag="ACHLCY_003189"
     CDS             11974..12729
                     /gene="ulaR"
                     /locus_tag="ACHLCY_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP0370391.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(12726..13475)
                     /gene="yjfP"
                     /locus_tag="ACHLCY_003190"
     CDS             complement(12726..13475)
                     /gene="yjfP"
                     /locus_tag="ACHLCY_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0370392.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITTRHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QHNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            13657..13986
                     /gene="bsmA"
                     /locus_tag="ACHLCY_003191"
     CDS             13657..13986
                     /gene="bsmA"
                     /locus_tag="ACHLCY_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP0370393.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            14135..14410
                     /gene="yjfN"
                     /locus_tag="ACHLCY_003192"
     CDS             14135..14410
                     /gene="yjfN"
                     /locus_tag="ACHLCY_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP0370394.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(14527..16152)
                     /gene="aidB"
                     /locus_tag="ACHLCY_003193"
     CDS             complement(14527..16152)
                     /gene="aidB"
                     /locus_tag="ACHLCY_003193"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP0370395.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(16236..17399)
                     /gene="yjfC"
                     /locus_tag="ACHLCY_003194"
     CDS             complement(16236..17399)
                     /gene="yjfC"
                     /locus_tag="ACHLCY_003194"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0370396.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(17402..18040)
                     /gene="yjfM"
                     /locus_tag="ACHLCY_003195"
     CDS             complement(17402..18040)
                     /gene="yjfM"
                     /locus_tag="ACHLCY_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP0370397.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(18050..18448)
                     /gene="yjfL"
                     /locus_tag="ACHLCY_003196"
     CDS             complement(18050..18448)
                     /gene="yjfL"
                     /locus_tag="ACHLCY_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP0370398.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(18466..19125)
                     /gene="yjfK"
                     /locus_tag="ACHLCY_003197"
     CDS             complement(18466..19125)
                     /gene="yjfK"
                     /locus_tag="ACHLCY_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP0370399.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTIAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(19176..19874)
                     /gene="yjfJ"
                     /locus_tag="ACHLCY_003198"
     CDS             complement(19176..19874)
                     /gene="yjfJ"
                     /locus_tag="ACHLCY_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP0370400.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(19893..>20193)
                     /locus_tag="ACHLCY_003199"
     CDS             complement(19893..>20193)
                     /locus_tag="ACHLCY_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP0370401.1"
                     /translation="DYGDLQINILFTSRQMIIETFICPVSSISNPDEFNTFLLRNQKM
                     MPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEEYSH"
BASE COUNT         5175 a         5269 c         4731 g         5018 t
ORIGIN      
        1 aattactaca aaaaaactca ctccaatatg atgctatatg tcgacaccat atgatttccc
       61 cattgcaatc actaaccttt ccatttagat ttatactggc atctctcttt tttgataaat
      121 aagggggatt gttttttgaa atatattcaa cataatttga tgccgatttg tttattattg
      181 gcatatgttt gtatcctttc tataaatttt ccgcctgaca gtggaaataa tatattctac
      241 tgatttttgt agtttaaacg atttatgaag aattttactg ttgtacagta ccagatactc
      301 agccagtttc agattgaaca tattcagcta ttcaaagagc aattcttcat taaactcaat
      361 aaattgttcc ctcagtgttc ggttaaaatg ctcacttatg gcatttattt tcggcgtata
      421 gagataagtc catacgaacc cgacggtggc ttcctgtagc gttttgtcga attttcccga
      481 gaactctttg cccatcatca gtgatgatct ggttgatacc aacaaggaac aactgggcta
      541 cacagcagaa gaaatgcctg atgatatcac ttttaagcga tagtacggcc agagagtagt
      601 tgctgtgttc gtcgagcatg gtaatgaaat aacggtacag ctcgccattc tgagctcaat
      661 agtatccatg ccactgagct tacaggtctt tatcgggcga tagtgcttta ttcttcgggg
      721 ctttgtggat gatttttcga aatgttcatg atagttaggt aaagctaccg catatcattt
      781 gcaaaatgac agatagatat acttcaattt gaatcttaac aattagtatg ggaatgctgt
      841 caatcctctg tatgcgtcca ccacatattg aaatttttgt tctctaatat attccacggg
      901 cgtcaagagt gtgaggcctg tgctgttata cagcaccaga cgttgtcccc catctttgac
      961 ctgtctcttg atcagatctc ctgatcaaga gacttcatca ccaggtaacc ctcagccata
     1021 tcctgatgtc tgaaccagcc atcccacata actacccaac cggggctgct ttgcccggac
     1081 attgcgcccc ggctggctga ccgtacccgc tgctgacttt gacatgttgc tgaccttcaa
     1141 atcctcaatg actatcattg cgtggttttt gctgacggtc gttgtgactt tatgaaggta
     1201 gtccctgcgg atatttgcga tacgggaatg cagtcgctgt attttgcgtt tctgcttctg
     1261 ccagttgttg ctgaatttga ccctgcggct taactgtcgc tgaagtctcg ccagcgtctt
     1321 ctggtttttc tgaaaactgt ttacaggctc aaagactgtg ccatctgaca gcgtggcgag
     1381 tttagccacg ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagtggatac
     1441 ttcactttct gtctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt
     1501 tttcacaact cccgtgacct gacggctatt ccggtagcgc atccagccaa gtttcggcag
     1561 aaaaatacgg ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcatcatt
     1621 ctgtccccgc tttttgaatc ggggaaaagc agcccgattc tggaagaagt ttttgtaagc
     1681 ccgctcaagg tctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg
     1741 cgtttcagtg gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta
     1801 tttattaccg gcctcatgat tctcattctg acgtgccaga gcacgattga aaacgaaacg
     1861 acatgcgcct gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaactggaa
     1921 tttaaatgcc tgtagtcgtt ccacaggtca aattatattt tggtctgtga aaaaagaaac
     1981 cgatatccgg cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac
     2041 caaataccga cgccagattt ttgaccatga cgcgacagaa aaactacgca cttacttttc
     2101 aaatgtatgt gctgattttg aagctgaact ggttgaaatg gatggcgaac cagatcacgt
     2161 ccatttgtta attaactatc ctcccaaact ggcgatatcc agtctggtaa acagcctcaa
     2221 aggcgtatcg ggtaggttac tgcgacgaga tcgaccagat attgcagtca ggtattacta
     2281 caaaggcgtt ttgtggagtc ctggctattt tgccaatagc tgcggaggtg cgccaatatc
     2341 cgtcatccgc caatacattg aacaacagca aacacctggt caggtggaaa accgcgcctt
     2401 atatccccgc cctgaaggac ggggttttac ggcgcaccag ataaaccagg ccaagcgtat
     2461 gctggttgat caaataatat cccccaagcg cagcaatatt aatattcacc tgcaatgggt
     2521 cgatacctgg cttaaaaata ttgtatttct tcccttcatc caataaggat tccattaact
     2581 gcaagtgcgc atggttaatt tctaagagac gctgtgattt tgcataatgc acacctttac
     2641 tttgattttc actatgcaca atttttaaaa accaagggtt agcaatataa taaccccatg
     2701 taaaatcaat aattttctca atggcttttt gtggctggtt aaccagatga ttaacaaacg
     2761 caccgatacc aacagcgtag aataattaca taccgcacaa caagaatgta tggtggaagg
     2821 cgtggaagtc attttctccc cgggggcatc gttatatcca taatgagcga tttttgatat
     2881 taatatcttt caccatccgc tattttcact acttcagaaa acaaacttat ttttattaat
     2941 atcgattctt tttctgtgaa catcagctgc gatatttata aaaacattaa acaggtaaca
     3001 ttacaatgac attaccaacg actatttatt catttcctgc atattcaccc catttttcgt
     3061 ccaccgacaa accagtaaaa ttaaaatttc atcaatatgc acgtgctaca ttactgccaa
     3121 atcgtggaag agatcataat tgcgatggca gacgtactgt tgagatccac aaactagatc
     3181 tctctgactg gcaagcattc aataagttag caacgaagtg taatgcttac gatggagtaa
     3241 caatgaatgg tgataattct tttggatgga accatgaagc tacattagat aatattcaca
     3301 cccagaaata caataaagct cacgcgggtg cgaggttaac cgcagaactg aaatatttat
     3361 tgcaagatgt cgaatccttc gaacccaaca gtaaatacac aatacacgaa gtggttttgg
     3421 gacctggtta tggtactcca gactataccg gccaaacgat aggatacgtt gtaacccttc
     3481 ctgcccaaat gcctaattgc tggtcatctg aactaccaac aatcgatctt tatatcgacc
     3541 aacttcgcac tgttacaggc gtttctaatg ccctcggatt catcattgct gcattgctaa
     3601 acgcgtattc tgatttaccc catgatttga aaattggtct acgttcctta tcaaattcag
     3661 cagcaatcta ctcgggttta ggtttcgagc gagtgccgca ggaccgagac ataaggagtg
     3721 atcgtatgca tctaacgcca gcaaaccatc ctgacttatg gacacaagag aatggtgaat
     3781 ggatctactt gcgaaactaa tacctgacga aaaatagaaa gcaaaacgcc gaccaatggt
     3841 cggcgttttt acgtctcgtt gaataacgaa ttattcagct actacgttta cgatcacttt
     3901 cgcgaatact tcgctgtgaa cctggaagct cacttcgtgt tcgccagtgg tacgcagaac
     3961 gccgttcggc agacgaactt cgctcttagc cacttcaacg ccagctgcag ttacagcgtc
     4021 agcgatgtcg cgagtaccga tggaaccgaa cagtttacct tcgtcgccag ctttagacgc
     4081 gatggtaaca gtttccagtg cattgatttt ctcagcgcga gcattagcag ctgccagaac
     4141 ttcagccagt ttagcttcca gttcagcgcg acgtgcttcg aagaattcaa tgtttttctt
     4201 ggtagctgga acagctttac cctgcggtac caggaagtta cgagcatagc ccgctttaac
     4261 gtttacctga tcacccaggc tacccaggtt tgctacttta tcaagcagaa taacttgcat
     4321 taccttatcc tctcaaagtc gtattaatgg accgtgaccg attactgatg gcgatcagtg
     4381 tacggcagca gggacaggta gcgagcgcgt ttgatagcgc gagccagctg acgctggtat
     4441 tttgcacggg taccggtgat acggcttggg acaatcttac cgctttcggt gatgtagttt
     4501 ttcagcgtag cgatatcttt atagtcgatc tcttgaacgc cttccgcggt gaaacggcag
     4561 aacttgcgac gacggaaata acgtgccata tggctagtct ccagaatcta tcaattcaat
     4621 ctgctcggca tgcaaaacca ttttgctcag tccgttcttt gccttgtggc atgaaatgaa
     4681 cccctgaacg gttatgcgac tgccgaccgt tatactgtga gtaatggcct ggttttcgtg
     4741 tccgctaaca ataacgggca tttgacacca tgcctgccgg tgaaagccgg cttcctcctg
     4801 cacagaacga tgctcaagca cgaactggca gtgaggaatt cctgatggac tgacctttcg
     4861 aaggggagtc ctgcacacgg tgccggacaa caccagacgg ttggtcatca gaaattactc
     4921 ttcagaatcc ccagcttcag catcatcagc ggtttcgttt gcgaaatcat cgcgacgctc
     4981 acggcgctcg tctttcgctt taaccatcgg agatgcttcg gtaacagcgt gcttggtacg
     5041 cataaccatg ctgcggataa cggcatcgtt gaagcggaag gtagtttcca gctcatcgat
     5101 cacttcctgc ggagcttcaa cgttcatcag aacgtagtgt gctttgtgca gtttgttgat
     5161 cgggtaagcc agctgacggc ggccccagtc ttccagacgg tggatcttgc cttctgcacc
     5221 agtgatggca gcagtgtagc gctcgatcat gcccggaacc tgttcgctct ggtcaggatg
     5281 gaccataaaa acgatttcgt aatgacgcat cgaattgctc cttacggatt attcagcctc
     5341 ctgtctgggt cagccgaatc ccggggaggc aaggaacgtg ttaaaggtcg gctgaaaaat
     5401 gacgcgttat agtacttgta tgccccgcta aactcaaggc cagccgacaa ataaatctca
     5461 tctttcgctc aaaagaacta actccctgat ttgaagttca tcacattccg tcacagtgtt
     5521 ttttcgaaca actgcatcag aaatggtcac catcaacttc tttatcattg attaaaatta
     5581 aatcagaaga tcatcacaga ggagagcaca caaagtcagc cgtcgaggag gtatctatgt
     5641 tcagtcgtgt tttagccctt ctggctgtgc ttttgctaag tgcaaataca tgggcagcca
     5701 ttgaaatcaa taaccaccag gccagaaata tggacgatgt gcaaagctta ggcgtgattt
     5761 atatcaatca taatttcgcc actgaaagtg aagcacgtca ggcattaaat gaagaaacag
     5821 atgcgcaggg cgcaacgtac taccacgtaa ttctgatgcg ggaaccgggg agtaacggca
     5881 atatgcacgc cagcgcggat atttatcgct agcaccaggt ataaccaacg aaacattgcc
     5941 atagtttgct ttgccccctt cgcagggggc tttttttgaa aaacggctgc ggaattagac
     6001 cagttatctc ccgaagaagg aaatttccgc agcgcgtgtt ttacttctgc ccgtaataag
     6061 cgttaggacc atgcttacgc atgaagtgtt tattcatcag gaagctgtcg atgtgattga
     6121 gttgtggatt aatactgcgc gcaatccacg ccattttcgc cacttcttcc atcactaccg
     6181 cgttatgcac cgcgtcgtgg gcatctttcc cccaggcgaa cggtccgtgc tgatacacca
     6241 caattcccgg cgtatgcagc ggctcggcgt tgcccagcgt ttcgataatc actttgccag
     6301 tgttcagttc atactcaccc tgcacctctt cttcgcttaa tccacgcgta cacgggatgt
     6361 caccaaagaa gtagtcagcg tgcgtggtgc ctaacgccgg gatcgccagc cccgcctgcg
     6421 cccatgcggt agcatgagtg gagtgggtat ggacaatgcc gccaagcgac ggataacgac
     6481 ggtagagttc aagatgcgtc gcggtgtcgg aagatgggcg atactcccct tccaccacct
     6541 taccgctcat atcgaccacc accatatcgt ctgctttcat ggtttcgtag gcgacgccgc
     6601 tgggcttgat caccaccagc ccgcgttcgc ggtcgatagc gctgacgttg ccccaggtaa
     6661 aggtcaccag cccgtagcgc ggcagatcca tgttggcttc aaatacctgc tgttttagct
     6721 tttgcattat gccgcctcca ccatgcccgc tttcgccatg cgcgctttca cccaatcacg
     6781 cgctttcgcc acttctgccg ccgggtcttc cgccgtttcg ctccacatct caatcaggta
     6841 cggcccgcaa tagccactct gtttgagcgt ttcgaaacaa cgttcgaaat ccactacacc
     6901 ttcgccaaac ggcacgtttt tgaagacgcc aggtttggtg tctttcacat gtaccgcgac
     6961 gatatgcccg attccggcct gcaactccat ctgcacatcg ttgtcccacg ccgacaggtt
     7021 gccgatatcc gggtagagct ggaaccacgg gttgttgaga tagtgcgcgt aacccagcgc
     7081 cttgctgatg gagttcatca acggataatc catgatctcc atcgccagcg tcacctgcgc
     7141 gcggctcgcc atctcaacgc tctctttcag gccgtcacgg aaacgacgac gcgtttcgtt
     7201 attggcttcc tgatagtaaa cgtcatagcc cgccagctgg atcacgcgaa tgccgacgtc
     7261 ctgggcgaac tggatagctt tacgcataat ctccagcccc tgcgcccgca ccgcgtcatc
     7321 ttcactgccc agcgggaaac gacgatgagc agaaaggcac atggacggca cgcgcacgcc
     7381 ggtttcaaca atcgcattca ccagcgccag acgctgctcg cggctccagt cgaggcgcga
     7441 caggcgctcg tccgtttcat ctaccgacat ctcgacaaaa tcgaagccca acgtttttgc
     7501 cagttgcagg cgttccagcc agcactcccc ggcggggagc gctttttcat agatgccaag
     7561 cgggatttgt ttggacaaca tatccgctcc ttagccccac agttcggcga tggaacgttt
     7621 gaactgacgt gcagcttcaa ccggagaagc ggcatcacgg atgctgcgac cagcgataaa
     7681 cacatgaatc ggaatacctt tgaacagcgg cagatcttcc agcgccaggc caccggtgac
     7741 ggtgactttg aagcccatat cggaaagacg tttgatcgcg gtaatgtccg cttcgcccca
     7801 cgctacgcct gcggcctgcg cgtcacggct gcggtgataa accacctgct gaatgcctgc
     7861 gtcacgccac tgctgcgcct gttcccaggt ccagtagccg gtcaattcga tctgcacgtc
     7921 gccgttaaac tcttttgcta cgtccagcgc gcctttggcg gtgttgatat ccgcacagca
     7981 aattaccgtc acccagtcag cgttggcttc gaagcacata cgcgaaagga ttttgcccgc
     8041 atcagcaatt ttagcgtctg ccagtacgat tttgtgcggg tagagcgctt tcaggtcacg
     8101 aaccgcacgc acgccttcgc ccacgcacag aatggtaccc acttcgataa tgtcgacttc
     8161 ttcggcaatc aggcgagtgg tttcgtaggc gctatccata gtctggttgt ccagcgcgac
     8221 ttgcaacatc ggtaatgaca tcttcaattc cttcttaagc tgccgcgttg gtgcggtcaa
     8281 tgagatccag tacttcctgc tcggtacggc aggcacgtaa acggtcgaaa ttttcttcat
     8341 cttcaaacag gttgacgatc tgcatgatgc ccacttcctg gtgcgtgttg gcatcgaccg
     8401 ccgccatggt gatgaggata tccaccgggt cgttatcttc gtggttgaac tccagcggct
     8461 ttttcagcgt caccagcgca aaaccggttt tcttaacgcc ctcttccggg cgaccgtgcg
     8521 gcattgccag gcctggtgcg agcacaaagt acggaccgtg ctgttcaacc gcatccagaa
     8581 tcgcctggta gtaacgcggc tcgacaacat ccgccgccac cagcaggtca acgccgattt
     8641 ttactgcgtc ctgccatgtc tcagcttcag cctgcaggcg gatggattta ttctccgcca
     8701 gcgaatcacg taatttcatg gcgcgtcctt acttcacatc ctgcgggaaa tgcgctttga
     8761 tcacttccag cagtttcggg ccaaagtcgg caggagagag catgttgcgc acgccaacca
     8821 cgtatttgtt gccggtcacg gtgatttcgc ccgcaatgtg cgtagaagcg atgatgatat
     8881 ccgcgccgct caactcgctt ttgtattcgc caaccgcgca gctatttacc gtgtggtcaa
     8941 tgtttgattg ggttaaaaac tggtccactt tcatcttcat gatcatggaa ctgccttgtc
     9001 cgttgccaca cacagccaga atacgtacgg tcataatcaa aactccttat taagcagact
     9061 gttctgccag ttgtttttct gcatcttctt ctgcgcgcag tgcgcggcca gcgaagaaca
     9121 tataagccag tgcaattaca atgatgacgg ccataaaggc gataccgatg gagaagaagc
     9181 cctgcatcat cggcggtgcc agaatcgacc agtccgccat gcccatccag gcgctcatac
     9241 cggtgagttt caccgcccag acgcagccaa agatttcaat catccccatc accagacata
     9301 tcttcagcgc cgcacgccag ccgccgaagt ggttagcgaa cacgccgatg gtggcgttgg
     9361 agaagaacat cgggataaag ccgggaataa tcaggatcga ggaaccgcag gcgaccagga
     9421 tgccaaccgc aatcagctga ccgatggtgc cccacataaa gccccaaacc acggcgttcg
     9481 gcgcgaagct atagatagcc gcacagtcaa tcgccagaac cgcaccaggg atcaggcgct
     9541 gggagatacc gttaaatgct tcagagagtt ccgccacaaa catgcgcacg ccctgagtga
     9601 tgatgaagat cgccaccgcg aaggagaaac cagtttgcag gatgtacacc gtccagttca
     9661 ctttgcctgc catcgcctgc acggtgtcga taccgaagga gagcagaatg gcaccaaaga
     9721 agatggtcat cacaatcgcc gtggagacga tgttgtcgtg gaaaatattc agccagcctg
     9781 gcagtttgag gtcttcaacg ctttcctctt ttttgccgag gaacggcgca actttatagg
     9841 caatccatga tgcaaactgt tgctggtgac cgatggagaa accgcagccg tccgtcactt
     9901 cctgagtcgg cttgtacatc atgttggagg taatgcccca gtagagcgaa accagaatcg
     9961 ccgtgcagat aatggttgtc cacatggagt agccgaaaat aaacaacgta acggcaatca
    10021 gccccgcctg ctggaacatg atatgaccag tcagcatgat ggtacgaata ccggtaatgc
    10081 gacgcagcag cacgtaacag atgttcagcg ccagcgctaa caacaccgcg taacctaccc
    10141 agctataggc atcgcccatg cggtcgatgg ttgccatcat cgacgcgtaa gtatcggaaa
    10201 ttgcgccgtt aatgccgtag acttcggaca ttttcgccac caccggtttg aaggtgctgg
    10261 taaggatgcc ggaccctgcc tgcaacaaca tgaaaccaat tatggtttta atcgtgcctt
    10321 taataataac gctgacactt ttgcgcagta ggatgtagcc cagacaggtc acaataccca
    10381 gcaacaacgg ggcattggtc atgacctggt taaaaaacac ggtaaagatg ttgtagagga
    10441 tctccataac gatctcctga agaagaggta accgggtatt ccgcacaccc ggaatgttgt
    10501 gcactcactc taattttcaa aagtaatcac aacaagatta tttatgatta aatgtgacgc
    10561 gacccgcaaa taattccaca cggatagagt gggattcaca caacggaaca attatttatt
    10621 taaattcatt tagttaattt aactacacat tccattaaat ctttccacac tcaaaatcaa
    10681 caacaacttc cctggcaaaa agtcaaaaaa tggtcattgc cttcctgaaa aaatagtgtc
    10741 aggattaatc aaaaccaatc acatattgat tcgaattgaa ctatcatgaa ggtaaatggc
    10801 gatgagtaaa gtgaaaagta tcacccgaga atcctggatc ctgagcactt tcccggagtg
    10861 gggtagctgg ctgaatgaag aaattgaaca agagcaggtc gcccctggca catttgcgat
    10921 gtggtggcta ggctgcaccg ggatctggtt gaaatcagaa ggtggcacca acgtttgcgt
    10981 tgatttctgg tgcggcactg gcaaacaaag tcacggtaac ccgttaatga aacagggtca
    11041 ccagatgcag cgcatggctg gcgtgaaaaa actgcagcca aacctacgca ccaccccgtt
    11101 tgttcttgat ccgtttgcga ttcgccagat cgacgcggta ctggcgactc acgatcacaa
    11161 cgatcatatc gacgttaacg tcgctgctgc cgtgatgcag aactgtgctg atgacgtacc
    11221 attcattgga ccgaaaacct gcgttgattt gtggattggc tggggcgtac cgaaagagcg
    11281 ttgcatcgtg gtcaaaccgg gcgatgttgt aaaagtaaaa gacattgaaa ttcatgcgct
    11341 tgacgctttc gaccgtactg cactgatcac cctgcctgcc gatcaaaaag cggctggcgt
    11401 actgccggac ggcatggacg atcgcgcggt gaactatctg ttcaaaacgc ctggcggctc
    11461 cctgtatcac agcggcgact cccactactc taactattat gcgaagcacg gtaacgaaca
    11521 tcagatcgat gtggcgttag gatcgtatgg tgagaacccg cgcggtatta ccgacaaaat
    11581 gaccagcgcc gatatgctgc gtatgggtga agccctgaat gcgaaagtgg ttatcccgtt
    11641 ccaccacgac atctggtcaa acttccaggc cgatccgcaa gagatccgcg tgctgtggga
    11701 gatgaaaaaa gatcgtctga agtatggctt caagccgttt atctggcagg ttggcggcaa
    11761 atttacctgg ccgctggata aagacaactt cgagtaccac tatccgcgcg gtttcgatga
    11821 ttgcttcact attgaaccgg atctgccgtt caagtcattc ctgtaatctg tcgtttatac
    11881 cggatagcgt ggagaagttc gcgctatccg gcgtagtatt tcctggctat ttcaaatatc
    11941 atctaaaaaa atcaaatttt atcggaatag ctcatgactg aagcacaaag acatcaaatc
    12001 ctcctggaaa tgctcgcaca attgggcttt gtgaccgttg agaaagtcgt tgagcgtctg
    12061 ggaatttcgc ctgccactgc gcgacgcgat atcaataaac ttgacgaaag cggcaaactg
    12121 aaaaaagtgc gcaatggcgc agaagctatt acccagcagc gcccgcgctg gacgccgatg
    12181 aatctgcatc aggcgcagaa tcacgatgaa aaagtacgta tcgctaaagc agcctcgcag
    12241 ctggttaatc cgggcgaaag cgtagtcatc aactgcggct ccaccgcgtt tctgcttggg
    12301 cgggaaatgt gtggcaagcc agtgcaaatc atcactaatt atctaccgct ggcaaattac
    12361 ctgatcgatc aagaacatga cagcgtgatc attatgggtg gacagtacaa caaaagtcag
    12421 tccatcactt taagcccgca gggcagcgaa aacagtctct atgccgggca ctggatgttt
    12481 accagcggaa aagggctgac cgcagaaggg ttgtataaaa ccgatatgct gacagcaatg
    12541 gcagagcaga agatgctgag cgtggtaggg aaactggtgg tactggttga tagcagtaag
    12601 attggcgaac gcgcgggaat gctttttagc cgtgccgatc aaatcgatat gctgatcacc
    12661 ggcaaaaatg ctaacccgga aatcctgcaa caactggaag cgcaaggggt cagcattctg
    12721 cgtgtttaaa gatgctggcg gaaaaatgtc acggcagcat ctaacgcctc aggcgtaatg
    12781 cggtgacgca cgcctggctg ccatgaacag gttagcagtt tatcccgtcc cgtctcgctt
    12841 aaggcctgct gcaaacgtag tgattcgtcg gcaggcacaa cgtcatcgtc gaggccatgc
    12901 cacagcagta gaggtctgtc cccaagttgt tccaggtggt ttgtcgcttc ccactctgcc
    12961 agcggcgcga taatgttatt gaattcattg tgctgtgctg ccgtttcagg tatcagcggt
    13021 ggaaacagtg aacgggcgag cgatgtaaaa tagcccgatc ccatcatgct ggcggtacat
    13081 ttcaccgtgg ggtggcgagt ggtaattccc agtgccgtca tcgcgcccat cgacgcgcca
    13141 ccgactgcca gacggtcatc aagcagccag ttttcttcgg ctattgccgc acgtaaagta
    13201 gtgaattcct gcatactttg tagcaagatt tgccagaatt gatttaaccg ccgcgcttcg
    13261 tcgctactaa aacggctacc gtgatcgggc gcatccggca tgatcacccg caaaccagcc
    13321 tgcgccagcg caacggcaaa atagctatac accaggctgg atgaagtaaa gccgtgataa
    13381 aaaattacgc acggtaacgg ggtatctttt tgcccgacag gataagcatg aagaacggga
    13441 atatctgcca gctcgcgtga ttctatttca atcatctgtc tctccttttt gtctggtatg
    13501 acatgaagaa atccttagca caagtttctg ctggcggtat tagttcataa tgttgagatg
    13561 tgggttacgc tttcgtcacg ttttcattcg aaaatcgcgt accaggtaac aattcgggaa
    13621 cattccccca aagttaaatt aataaggcac tacactatgg ttagcaggaa acgtaatagc
    13681 gttatttacc ggtttgccag tttattattg gtgttgatgt taagtgcctg tagcgctctg
    13741 caaggtacgc cacagccagc accgccagta acgaaccatc cgcaagagat tcgccgcgac
    13801 cagacgcaag ggttacagcg aataggtagc gtaagcacga tggttcgggg ttctccggat
    13861 gacgcattag cagaaatcag agcaaaagct gtcgctgcaa aagctgatta ttacgttgtc
    13921 gtcatggtgg acgaaaccat cgtgacagga cagtggtatt cacaagccat tttgtaccgt
    13981 aaataaatca gacaacttac atagtcagct ttacattgct ttgcgtccct atgcgtttac
    14041 ctgtgcacaa taaattaccg gctggaagcc tgggcgttta ttaccgcgat ggaatgccct
    14101 gcaacatgtg gggaaacgaa aaatggagct gacgatgaaa caattacttg cctcaccctc
    14161 gctgcaatta gtgacttatc ctgcgagcgc cacggcacag tctgcagaat tcgccagtgc
    14221 tgactgcgtg acgggcctga atgaaatagg tcagatctca gttagcaata tttcagggga
    14281 tccgcaggac gtggaacgta tcgtggcgtt aaaggccgat gaacaaggtg cgtcatggta
    14341 ccgcattatt accatgtacg aagaccagca gcctgataac tggcgcgtac aggcgatact
    14401 gtatgcatga gtgttatata cagcaaatga acaatgccgg atgcggcgcg agcgccttgt
    14461 ccggcctaca attttcgcat atttcattga gttaatcgaa accagcgtcg catcagtcgt
    14521 atacgcttac acacacactc cccccgtcgc ccgcagcaat aaatcattct ggatctgctc
    14581 tgacaaccgt acgccgccgc gcgtatctaa catcacctga caccacgcct gcgccattgg
    14641 cggagaggca tatttcaaca tttgcgcacc gcagcccagc aggaatagct gatgagtaat
    14701 ctctcgcccc agttcttcag ctggcttgcg cagctgctgc tgtaaacgac gaaccgcgcg
    14761 atcaaaatag cgatcctgcc ctttcacttc cacaaacgct tccgacaata agtcgtatac
    14821 gcccgcttgc ttattgagaa cgcgcagcac atccaggcac ataatattgc cggaaccttc
    14881 ccaaatactg tttaccggca tctcccggta aagccgcggc agctcgctct cctcgcaata
    14941 accaatgccg cccagcacct ccatcgcttc ggccacaaac ggcatacctc ttttgcagat
    15001 cacaaatttt gccgcaggcg taaataaacg cgcccacagg gcttctttgg catcggcacg
    15061 ccggtcccac gctcgcgcaa gacgaaacag caacgccgtt tgcccttcaa gctgaagtgc
    15121 catgcgactt aagacatgac gcataagggg ctgttggatc aatggattac caaaaacatg
    15181 gcgttgatgt gcatgataaa tcgccagcga aaatgcacgg cgcatcatgg catggctacc
    15241 cagggcgcaa tcaaaacgcg tcatcccacc cattttcagg atcagacgaa ttccttcccc
    15301 ttcctgcccc aacaaccagc caatggcatc ctgaaactcc acttcgcagc tggcgttaga
    15361 gcgattaccc agcttatctt tcagccgctc gaggcgaatc gcgttgcgtt gcccgtcagg
    15421 caaaaagcgc ggcacaaaaa agcaggacaa accgcccgta gtctgcgcca gcaccagatg
    15481 cgcatcgctt tgcggcaccg agaaaaacca tttatgcccc accagccgat aagagccatc
    15541 ttccaggcgc tctgcgcggg tggtgttgct catgacatcg gaaccgccct gcttttccgt
    15601 cattcccatg ccaatcaaca aaccgcgttt ttgcccacct ggcaataagt gagaatcgta
    15661 gcgatcgctc agcagcggcg tggtccagtc ctgaaacggc gtgggtaaca tctgtaacaa
    15721 caatggcgtg gcggcaaagg tcatcgttat tggacataac gacccagcct caacctgcgc
    15781 atgtaacata aaacgcgccg cgcgcgccac aaatgcgccg gagcgagcgt cttcttccca
    15841 ggcaagattg tgcacccgat tggtacataa cgcctgcatc agcaggtgcc aggcggggtg
    15901 aaaacgcaca tcgtccagac ggcgtccttg cgcatcgtag cgcaataatt caggcggatt
    15961 cacattcgcc agccgcccca gttcaagaga ttcagccgtt cctaactgct gaccaatact
    16021 ggctagaaaa tcgctatccc agccagcacc ttcacgcgtt accgcttcac agagcgcgcc
    16081 atcagacagg tataagttgc tgttatttaa tggtataggt tgattaaaaa cggtgtgagt
    16141 ttgccagtgc actgtgtctc cctccatcaa tggcagtcac ccttagtatg gtcactgcca
    16201 tggattcctg acagaaagag attgctaaaa cattcttatc cagcaatgta atgcggaacg
    16261 aaacgtgagg tgtctttggt gatcagcgtg ttatcttcac ggatccccat tccgcacgct
    16321 tcatcatcga caatccagct accgatgagt gtgtagctat cgccaaatcg cggcagaggt
    16381 tgaaacgcct ggtagatcat cggttcatcg gcgtaatcac catcagcgtg gtcaacgacg
    16441 ttattctgac cgtcgaaaat ggtgacgttt ccgccttcgc gcgagtagat cggtttacgc
    16501 acatagcttt cgccagcggc aatctgcggt ttttcgccct caaaccagga tgcaagaagg
    16561 ttaggatgac cagggaagaa gcgccaaagc agcggcatta gccctttatt actcaagata
    16621 cttttccata acggctccac ccattgctcg cgacgcttgc gcagcagcgg accgttatcg
    16681 tcacgcatca tccactccag cggatacagc ttaaatgcac gctggatgac attatcatca
    16741 agatcggtca gtacaccgcc gacgcccaaa ccgagatctt caatgtagat aaaccgcgac
    16801 tcctgccctg cctgctgggc gcagtcctgc aagtacagca cggtagtcct gtcttcatcg
    16861 gtgtcctgac agcagcagaa ataaaacggc tcccggctat aaagctcgct aaagcgtgaa
    16921 atcaggcgtt cctgaatggc attgtactga tcggcatcac gcggaataat gccgctgcgc
    16981 cgggcatcct ccagccacaa ccactggaaa taagccgact catacaacga ggtcggcgta
    17041 tcggcgttgt actccagcag cttcaccggc gcattgccac accaggcaaa atccatccgg
    17101 ccatacagcg aaggatcgcg tgcgcgccag ctttcggcga tcacatccca gtacaacggc
    17161 gggatcgcca gttgcgtcag gatctcttcg tctttaaccg cgcgatcaac cacctcaagg
    17221 cacatctgat gcagttccgc agtcggtttt tcgatctgct cttcaatctg gcgcagagta
    17281 aaacggtaag cccgactctc gtcccaatag atttcattgt cgatgatatg aaagtcgaaa
    17341 ccgttgttag cggcgatctg gtccagatcc cgtcgcacag gaacgttgtg tctcagcatg
    17401 attagcctcc ccaatgccca cgggcgctgg aagaacgacc atagccgccg cgcgaaacgg
    17461 ttgacgcttt tttggtggta aagccgcctg aagagtaaga ctcttttttg ccagaaccgg
    17521 agcgccagga gtaatcaccg gaagtgttgc gccagacggg gcgggaagca aatgaggatc
    17581 cgccgctgtt atagacgaac ggctcatcgc gatctttacg aataacccgg cttaacaaaa
    17641 atccagaaac gaccgggatc caactctgtt caacattgtc gtagtagcaa ttttcgtact
    17701 tagactgaca attctgctga gtcatattct tcggaacatc ggcataaaat gccgctttgg
    17761 cgttgttcca gccacgcgcg caaatgtcgg cattattacc gtcatcaata caatcctgca
    17821 ctgtcgaata aaacgttccg tcgccgtcgt tatcgacatc gccactatcg ccacaacctt
    17881 tcaatacaaa aaaagcggca ccgcccatta gagccaacgt taagtatttt tgtgcgtagc
    17941 gattgcgacg cggttcaaaa ggattattcg gtctaccaat gcgactgatc gctccgtgac
    18001 caattttact gttatttctt gatttgcgtt ttctggccat gatcgtccat taccatgtca
    18061 tacaagctgc gttaaaaata ccgccagcca gcgcggcggt tcccataaac attcctgctg
    18121 cggtattgtg attaataatt ttttcgctta atgcgggcat ataaagtctg acaccagcaa
    18181 aaacgagtaa ctgaatcacc agtgcaatcc cgccccaggc gaaatagtct ggaatactca
    18241 ccgcattgat tgccgcactg gataatggga taacgtaacc taacaatgta ccactgaacg
    18301 ccagtgaagc cgcggtatta ttgtttttga ttaactgcca ttcgttgtgc ggtgtaattt
    18361 tagaatagat aaaaagaaaa ataatcacca tagccacgcc aataaaaaaa taggcgctaa
    18421 aggcaagaag tgaatccagt atatgcattg taaacgtcct tgtaattaac caataatatg
    18481 aagtgatgtc agcgggatat cgacacccaa cgcgcgcgaa aataaccact ctggctcgcc
    18541 gagatcgtta aaagattcct caccatttaa cagcaaatat tcgtaagtat cttcggttac
    18601 ttgccgctgg tagcccatgg taaaattatg gacctcccat ttggcatggt tttgattttc
    18661 tactttttcc agcatgtaga cgggttcgat atttcccggt tcttcgctgt taaaaaatcg
    18721 ctgccagcgt ttttcctgcc agtttaagat cattgccccc atcactttgg cgttgatcgc
    18781 ctcgcgccag tgactctctt tactgatacc gtagctctct tcatagacaa agagcttgat
    18841 atcatcaatg tcatcaatat cttcgccgcc ggtggtattg atttgcagaa attcatcgcc
    18901 tgaagtgtag tagcggaaaa tctgactacc gccgcccaga tcgatgtggc tgacagcggc
    18961 tatcgtaaat tcctcacccg gcagcgcaat cagcaattca tcttccagca aacgaaacgc
    19021 taacgtgtcg agcgtaaagc cactattgag atgaagtccc agcggaccgc gagcgatagc
    19081 aggcttatta tccttgccaa acagacgctg gaaaaaacca gacatatttt atccttaaaa
    19141 tagttgccag ccttttagcg aggctggcaa aaaaattact cgccctgttg gcgttgcagt
    19201 cttgctaata catcctgggc gctactttta ttgctaccgc caattccggc ttccgccagc
    19261 ttttcatcaa gatcgcgacc gtctgcgact ttttccaact gtgcagcagc atccaggcga
    19321 gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc tgtcgaaacg
    19381 ctggaagatg cgccaacggt agaggttgtt acagcctgtt gtgcacgctg catggcttca
    19441 gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc tgtcgctgta
    19501 actgcttttt ccacgccatc acgggagact tccagattcg acaacacttg ctcttcagcg
    19561 gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa cgacggatta
    19621 acgttcttgc ttaacgcttc cagcgcacgg gcttccagac tggctttgcg ctcacgtaaa
    19681 tctttcagct tatcgtgact taatttcacc cgcgccagca gatcaacgcg agattttccg
    19741 gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg cacgccctgg
    19801 gtttcttcaa tggattcttc cgcctgggag ataaacgatt tccccagcgt aaataaactt
    19861 tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc tgtaatttca
    19921 gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc ttcaagagaa
    19981 gatttaagcg ataacgcccc gaaaacaatg taatactctt cctgttgcac gctggagatt
    20041 cctaccgatg acaacggcat catcttctga tttcttaata agaaggtatt aaattcatct
    20101 ggattgctga tactacttac cggacaaata aaggtttcga taatcatttg gcgggaagta
    20161 aaaagaatat tgatttgcaa atcgccataa tca
//
LOCUS       DAZGTR010000080        19736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGTR010000080 DAZGTR010000000
VERSION     DAZGTR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.080"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(75..281)
                     /gene="ybfA"
                     /locus_tag="ACHLCY_003200"
     CDS             complement(75..281)
                     /gene="ybfA"
                     /locus_tag="ACHLCY_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP0370402.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            594..683
                     /gene="kdpF"
                     /locus_tag="ACHLCY_003201"
     CDS             594..683
                     /gene="kdpF"
                     /locus_tag="ACHLCY_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP0370403.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            683..2356
                     /gene="kdpA"
                     /locus_tag="ACHLCY_003202"
     CDS             683..2356
                     /gene="kdpA"
                     /locus_tag="ACHLCY_003202"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP0370404.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPDVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLDQHYLPLNPQQLPGLSWDLA
                     LNAAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2379..4427
                     /gene="kdpB"
                     /locus_tag="ACHLCY_003203"
     CDS             2379..4427
                     /gene="kdpB"
                     /locus_tag="ACHLCY_003203"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP0370405.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSARGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4436..5008
                     /gene="kdpC"
                     /locus_tag="ACHLCY_003204"
     CDS             4436..5008
                     /gene="kdpC"
                     /locus_tag="ACHLCY_003204"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP0370406.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5001..7685
                     /gene="kdpD"
                     /locus_tag="ACHLCY_003205"
     CDS             5001..7685
                     /gene="kdpD"
                     /locus_tag="ACHLCY_003205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP0370407.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLTDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7682..8359
                     /gene="kdpE"
                     /locus_tag="ACHLCY_003206"
     CDS             7682..8359
                     /gene="kdpE"
                     /locus_tag="ACHLCY_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP0370408.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
     gene            8521..8655
                     /locus_tag="ACHLCY_003207"
     CDS             8521..8655
                     /locus_tag="ACHLCY_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0370409.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            8634..8738
                     /gene="speFL"
                     /locus_tag="ACHLCY_003208"
     CDS             8634..8738
                     /gene="speFL"
                     /locus_tag="ACHLCY_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP0370410.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9049..11247
                     /gene="speF"
                     /locus_tag="ACHLCY_003209"
     CDS             9049..11247
                     /gene="speF"
                     /locus_tag="ACHLCY_003209"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP0370411.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11244..12563
                     /gene="potE"
                     /locus_tag="ACHLCY_003210"
     CDS             11244..12563
                     /gene="potE"
                     /locus_tag="ACHLCY_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP0370412.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYALVLPRFELKNKHG"
     gene            complement(12619..14259)
                     /gene="pgm"
                     /locus_tag="ACHLCY_003211"
     CDS             complement(12619..14259)
                     /gene="pgm"
                     /locus_tag="ACHLCY_003211"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP0370413.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14285..14830)
                     /gene="seqA"
                     /locus_tag="ACHLCY_003212"
     CDS             complement(14285..14830)
                     /gene="seqA"
                     /locus_tag="ACHLCY_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP0370414.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            15015..15779
                     /gene="ybfF"
                     /locus_tag="ACHLCY_003213"
     CDS             15015..15779
                     /gene="ybfF"
                     /locus_tag="ACHLCY_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0370415.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15919..16212
                     /gene="ybfE"
                     /locus_tag="ACHLCY_003214"
     CDS             15919..16212
                     /gene="ybfE"
                     /locus_tag="ACHLCY_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP0370416.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16352..16882
                     /gene="fldA"
                     /locus_tag="ACHLCY_003215"
     CDS             16352..16882
                     /gene="fldA"
                     /locus_tag="ACHLCY_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP0370417.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17092..17178
                     /gene="uof"
                     /locus_tag="ACHLCY_003216"
     CDS             17092..17178
                     /gene="uof"
                     /locus_tag="ACHLCY_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP0370418.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17171..17617
                     /gene="fur"
                     /locus_tag="ACHLCY_003217"
     CDS             17171..17617
                     /gene="fur"
                     /locus_tag="ACHLCY_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP0370419.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17701..18027)
                     /gene="chiQ"
                     /locus_tag="ACHLCY_003218"
     CDS             complement(17701..18027)
                     /gene="chiQ"
                     /locus_tag="ACHLCY_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP0370420.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18077..19483)
                     /gene="chiP"
                     /locus_tag="ACHLCY_003219"
     CDS             complement(18077..19483)
                     /gene="chiP"
                     /locus_tag="ACHLCY_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP0370421.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4518 a         4959 c         5261 g         4998 t
ORIGIN      
        1 tctgaacatc ttcctgatac aaaaaaccgc cagcgtttcc gctggcggtt ttcaatgtat
       61 tcaaaactca gcaatcaata aaaatcacca gttgcctttt cggcctgatc catccacacc
      121 ggtttatcgc tggttttcga ccagacgcga tgcaggtagc tgtaaaagcg ggcgcggtct
      181 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata agtacggcgt
      241 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      301 atctacccgg tgaatggtac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      361 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      421 cattcatcct gtaattaagt tacacaaaag ttaaattaat actaaacatt agttaaatca
      481 tggcttttgc catttttata ctttttttac accccgcccg cagatttttg cgaaatcttt
      541 gcagccagaa ttctaccctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      601 caggcgtgat aaccggcgta ttgctggtgt ttttattact gggttatctg gtttatgccc
      661 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgatcg ccacgttttt
      721 actggtgtta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
      781 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctgacgtctc
      841 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
      901 gctggcggtg ctgtttttta tgttgctcga tcagcactat ctgccgctta atccacagca
      961 gttgccgggg ctgtcgtggg atctggcgct gaatgccgcc gtcagctttg tcacgaatac
     1021 caactggcaa tcttacagcg gtgaaaccac gttgagctat ttcagccaga tggctggctt
     1081 aacggtgcaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccctcatccg
     1141 tgcgtttacc cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1201 cacgttatgg gtgctcgtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1261 tgcgctgcaa aactttcagc cttatcaggc tgtgaatacc gttgaaggag cgcaacagct
     1321 gttacccatg gggcctgtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1381 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1441 cgtgcagatg ctggcgatct tcttgatccc aacggcgctg tgctttgcct ttggtgaagt
     1501 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1561 ctgcgtaggc gtggtgatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1621 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     1681 cctgtttgcg gtcgtgacta cggcggcttc ctgtggcgcg gtgattgcga tgcatgattc
     1741 gtttaccgct ctcggtggca tggtgccgat gtggctgatg caaattggtg aagtggtgtt
     1801 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     1861 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga
     1921 gatgaaactg actgcactgg cgattctggt taccccgacg ctggtgctga tgggcgcggc
     1981 gctggcaatg atgaccgacg ccggacgtag cgccatgctc aaccctggcc cgcatggttt
     2041 tagcgaagtg ctgtacgccg tgtcatccgc cgctaacaac aacggcagcg cctttgccgg
     2101 attaagcgcc aactccccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2161 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2221 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2281 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2341 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2401 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2461 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2521 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2581 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     2641 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     2701 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     2761 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     2821 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     2881 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     2941 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3001 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3061 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg aggggcggta
     3121 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3181 gcggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3241 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3301 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatccct gcgcagggcg
     3361 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3421 aaggccgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3481 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3541 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat gttgaggcta
     3601 acggcggtca cttccctacc gatgttgatc aaaaagtcga tcaggttgcg cgtcaggggg
     3661 ccacgccgct ggtggtggtg gaaggttccc gtgtgctggg cgttattgcg ctgaaagata
     3721 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaaatgggc attaaaacgg
     3781 tgatgattac cggcgataac cgtctgactg ccgccgccat tgctgcggaa gcgggcgtcg
     3841 atgattttct cgccgaagcg acaccggagg ccaagctggc attgattcgt cagtatcagg
     3901 cggaaggccg tttggtggcg atgaccggcg acggcaccaa cgatgctcca gcgctggcgc
     3961 aggcagatgt cgcggtggcg atgaactccg gtactcaggc ggcgaaagaa gcgggcaata
     4021 tggtcgatct cgactctaac ccgaccaagt tgatcgaggt ggtacatatt ggcaaacaga
     4081 tgctgatgac ccgtggctcg ctgaccacct tcagcattgc caacgatgtg gcgaaatact
     4141 tcgccattat tccggcggcg ttcgcggcaa cgtatccgca gttaaatgcg ctgaacatca
     4201 tgcgccttca ttcgcccgac tccgccattc tcagtgcggt cattttcaac gccttgatta
     4261 tcgtcttttt gattcctctg gcgttaaaag gcgtgagtta taaaccgctt accgcttctg
     4321 ccatgttgcg ccgtaactta tggatttacg gtctgggtgg gctgctggtg ccgtttattg
     4381 gtatcaaagt cattgattta ctgctgaccg tttgcggtct ggtgtgaggt ttaccatgcg
     4441 tggcttacgt ccggcattat cgacatttat ctttctgtta ttgattactg gcggcgttta
     4501 cccgctgctg accaccgtgc tggggcaatg gtggtttccc tggcaggcca acggttcgtt
     4561 gattcgtgaa ggtgatacgg tgcggggttc ggcattaatc gggcagaatt ttaccggcaa
     4621 cggctatttt cagggtcgcc cgtcggcaac ggcagaaatg ccctataatc cacaggcttc
     4681 tggcgggagc aatctggcgg tcagtaaccc tgagctggat aaacaaatag ccgcacgcgt
     4741 tgctgcatta cgggccgcta acccggatgc cagtacgaac gttccggttg aactggtgac
     4801 ggcatcggca agcgggctgg acaataatat caccccgcaa gcggcggtct ggcaaatccc
     4861 acgcgtggcg aaagcgcgta atctcagcgt tgaacagctc acgcaactga tcgcaaaata
     4921 cagccaacaa ccgctggtga aatatatcgg ccagccggtc gtcaacattg ttgaactcaa
     4981 tctggcgctg gataaacttg atgaataacg aacccttacg tcccgacccc gatcgtctgc
     5041 tggaacaaac tgccgcgccg catcggggga agctgaaagt tttcttcggt gcctgtgcag
     5101 gcgtcgggaa gacctgggcg atgctggcag aagcccagcg actgcgggcg caagggctgg
     5161 atattgtggt tggcgtggta gaaacccatg ggcggaaaga taccgccgcc atgcttgaag
     5221 ggctggctgt tctgccgccc aaacgccagg cgtaccgtgg gcggcatatc agcgagtttg
     5281 atctcgatgc cgccctcgcc cgccgtccgg cgctgatctt aatggacgaa ctggcgcaca
     5341 gtaatgcgcc aggttctcgt catcctaaac gctggcagga tatcgaagaa ctgctggaag
     5401 ctggcattga tgttttcaca accgtcaacg ttcagcatct ggaaagtctg aatgatgtgg
     5461 tcagcggcgt caccggaatt caggtacggg aaaccgtgcc cgatcctttt ttcgatgccg
     5521 ccgacgacgt agtgctggtg gacttgcccc cggacgatct gcgccagcgg ctgaaagaag
     5581 gcaaagtcta tattgccggg caggcggagc gcgccattga acattttttc cgcaaaggca
     5641 atctgatcgc cctgcgtgaa ctggcactgc gccgtactgc cgatcgcgta gatgagcaaa
     5701 tgcgcgcctg gcgggggcat cctggtgaag agaaagtgtg gcacacgcgc gacgcgatcc
     5761 ttttatgcat cgggcataac accggcagtg agaaactggt ccgcgcggcg gcgcggttgg
     5821 cctcgcgcct gggtagcgtc tggcacgcgg tgtatgttga aacccctgcc ctgcatcgct
     5881 taccggaaaa aaaacgtcgg gcaattctca gcgccttacg tctggcgcag gagctgggcg
     5941 cggagacggc tacacttact gatccagcgg aagaaaaagc agtagtgcgt tatgcccgtg
     6001 aacataatct cggcaagatt attctcggtc gcccggcctc gcgccgctgg tggcgtcggg
     6061 aaacgtttgc tgaccgactg gcgcgcatcg cccccgatct cgatcaggtg ctggtcgcgc
     6121 ttgatgaacc acccgcccgc acgattaaca acgcgccgga tagccgctct tttaaagaca
     6181 agtggcgggt acagattcag ggatgcgtgg ttgccgccgc gttatgcgcc gttatcactt
     6241 taattgccat gcagtggctg atggcgtttg atgccgccaa cctggtgatg ctgtatctgc
     6301 ttggcgtggt ggtggtggcg ctattttatg gacgctggcc ttcggtggtt gctaccgtca
     6361 ttaatgtggt gagtttcgat ctctttttta tcgccccacg cggcacgctc gctgtctctg
     6421 atgtgcaata tctgctgacc ttcgcggtga tgttaaccgt cgggctggtg atcgggaacc
     6481 ttactgcggg tgtgcgttat caggcgcggg tagcccgtta ccgcgagcaa cgcacacggc
     6541 acttatatga aatgtcgaaa gctctggcgg tgggccgcag tccgcaggac atcgccgcca
     6601 ccagcgaaca atttattgcc tccacgtttc atgcccgcag tcaggtgttg ttgcccgatg
     6661 acaacggtaa attgcagccg ttaacacatc cgcaaggaat gacgccatgg gacgatgcca
     6721 tcgcgcagtg gagttttgat aaaggcctgc ctgcgggtgc gggcaccgac acgttacccg
     6781 gcgtgccgta ccagattttg ccgctaaaaa gcggcgagaa aacctacgga ctggtggtgg
     6841 tggaaccggg gaatctgcgt cagttgatga tcccggagca gcagcgcctg ctagagacgt
     6901 ttacgctgtt agtcgccaat gcgtttgagc ggctgacgct gaccgccagt gaagaacagg
     6961 cgcggatggc aagcgaacgt gaacagatcc gcaacgccct gctggcggcg ctttcgcatg
     7021 atttacgcac gccgcttacg gtgctgtttg gtcaggcaga aatcttaacg ctcgatctgg
     7081 caagcgaagg atcgccccac gcccgccagg ccagcgagat tcgtcagcat gtgctgaaca
     7141 ctacccgact ggtgaataat ctactggata tggcgcgaat tcagtccggc ggctttaatt
     7201 tgaagaaaga gtggttaacg ctggaagaag tagtcggcag cgcgctgcaa atgctggaac
     7261 cgggtttatc gtcgcccatc aatctttctc tgccagaacc gctgacctta atccacgttg
     7321 acgggccact ctttgaacgg gtgctgatta atctgctgga gaacgcggtg aaatatgcgg
     7381 gtgcgcaggc cgaaattggt atcgatgccc acgttgaggg cgaaaatcta caactggatg
     7441 tctgggataa cggccccggt cttccgccag gccaggagca gacgatattt gataagtttg
     7501 ctcgcgggaa taaagagtcg gcagtaccgg gggtagggct tggactggca atttgtcggg
     7561 cgatagtgga tgtacacggg ggcactatta ccgcgttcaa ccgaccggaa ggtggtgcct
     7621 gttttcgtgt tacacttccc cagcaaaccg ctcctgaact tgaagacttt catgaggata
     7681 tgtgacaaac gttctgattg ttgaagatga acaggctatt cgtcgctttc tgcgcacggc
     7741 gctggagggc gacgggatgc gcgtctatga ggccgaaacg ctgcaacgcg gcttgctgga
     7801 agcggctacc cgtaagccag atttgattat tctcgatctc ggcctgcccg atggtgatgg
     7861 gattgagttt atccgcgacc tgcgccagtg gagcgcggtg ccggtgattg tgctttccgc
     7921 acgcagcgaa gagagcgaca aaatcgccgc gctggatgcc ggagcggatg attatctgag
     7981 taagccgttt ggcattggcg aattgcaggc ccgtctgcgc gtcgctttac gccgccactc
     8041 cgccaccgcc gcgcccgatc cgctggtgaa attttccgat gttaccgtcg atttagccgc
     8101 ccgcgtgatt caccggggtg aggaagaggt gcatctcacg ccgattgagt tccgcctgct
     8161 ggcggtgctg ctgaataatg ccgggaaagt gctcacccag cgccagttac tcaatcaggt
     8221 gtgggggcca aacgcggtcg aacacagtca ctatttgcgt atttatatgg gacatctgcg
     8281 acaaaaactg gaacaggatc ccgctcgccc ccgccatttc attactgaaa ccggtattgg
     8341 gtatcggttt atgccatgaa tgtttattca ataaatgcgc ttaaataatt attaatttat
     8401 tattaaacct gccaaaaata ttattatttg gcaggtttaa tttcttaacg caaatataaa
     8461 acacaaaatt acaacattta aataacaaaa cagcatctaa tatggcgcta ttcataatta
     8521 atgatttatt atttggggta tgatcgtttt ttgttgatct tcttcacaga ttatagccat
     8581 ttcatggata gaataactct accttcaact gacacagcaa gaggtaaagg taaatggaaa
     8641 ataataaccg cttaatgcct catataaggc ggacaaccca tatcatgaag tttgcccatc
     8701 gtaatagttt tgactttcat ttctttaatg cccgctagtc ttctgactaa agggcacccc
     8761 aacgtacagg tctccctgac tttaagcatt acaggttaat acctgtattc ctcggtgctc
     8821 atatactgct aaccctttta aactctaaat aattcgagtc gcagcacttg caacttgagg
     8881 tatgacgagt atagccagtt accgggctgg tctgggttat tgcatctgca aaaagcaaac
     8941 tactgattta tttatcagcg gtggagcttt gctttttttc cggcgtgatc gatttctcct
     9001 ttgagaaatt gaggacctgc tattacctga aataaagaga tgaacaaaat gtcagaatta
     9061 aaaattgccg ttagtcgttc ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat
     9121 atagacgaaa gtaattatat tgacgttgcc gccattattt tatcagtcag tgatgttgaa
     9181 cgtggaaaac tcgatgaaat agacgctact ggctatgaca ttcctgtttt tattgcaacg
     9241 gaaaatgaag aacgtgtacc cgcagaatat ctgccacgta tttctggtgt cttcgaacat
     9301 tgcgaatcac gtaaagagtt ttatggacgt cagttagaaa ccgctgccag ccattatgaa
     9361 acgcaactgc gcccgccctt cttccgcgca ctggtcgatt atgttaatca gggcaatagt
     9421 gcttttgact gccctggtca tcaggggggt gagtttttcc gtcgccatcc ggcaggcaat
     9481 cagttcgtgg aatactttgg cgaaatgctc ttccgctctg acttgtgtaa tgctgacgta
     9541 gcaatgggcg atctgctgat tcatgaaggt gcgccatgta ttgcacaaca gcatgcggct
     9601 aaagtcttta atgccgataa aacctatttc gttttaaacg gtacttcatc ttctaataaa
     9661 gtggtcttaa acgcattgct gacgccgggc gatctggtgt tgtttgaccg caataaccat
     9721 aaatctaacc accacggtgc gttgctacag gctggcgcga caccggttta tctggaaacc
     9781 gcacgtaacc cgtatggctt tatcggtggc attgatgcgc actgttttga agaaagttac
     9841 ctgcgtgagc tgatcgcgga agtcgcaccg cagcgggcaa aagaggctcg tcctttccgc
     9901 ctcgctgtga ttcagttagg cacctacgac ggtacgattt ataacgcccg ccaggtggtg
     9961 gataaaattg gtcatctgtg tgactacatt ctgtttgact ccgcatgggt tggctatgag
    10021 caatttatcc cgatgatggc tgactgttcg ccgctgttgc tggatcttaa tgagaacgat
    10081 ccgggtattc tggttacgca atccgtacat aaacagcagg ctggtttttc tcaaacttca
    10141 caaatccata aaaaagacag ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc
    10201 atgaacaacg cctttatgat gcatgcctcc accagcccgt tctatccgct gtttgccgca
    10261 ctggatatca acgccaaaat gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt
    10321 gtggtaaatg gcattaatgc ccgcaagctg atcctcgata actgtcagca tattcgtccg
    10381 ttcgtacctg aactggtgga tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg
    10441 gttgatctgc gcttcttcca gtttgtacca ggtgagcact ggcactcttt tgaaggctat
    10501 gcagagaatc aatactttgt cgatccatgc aaactgttgc tgacaacccc aggtattgat
    10561 gcacgtaacg gcgaatatga agcgttcggt gtacccgcga cgattcttgc taacttcctg
    10621 cgcgaaaatg gcgtagtgcc ggaaaaatgc gatcttaact ccatcctctt cctgctgact
    10681 ccggcagaag atatggccaa gcttcagcaa cttgttgccc tgctggtacg cttcgaaaaa
    10741 ctgcttgagt ccgacgcgcc attagcagaa gtgctgcctt ccatctacaa acagcatgaa
    10801 gagcgctacg ccggttatac cctgcgtcag ttgtgtcaag aaatgcatga tttgtatgcc
    10861 cgtcacaacg tgaaacaact gcaaaaagag atgttccgta aggaacactt cccacgcgta
    10921 agcatgaatc cgcaagaagc caactacgcc tatttacgcg gtgaagtgga gctggttcgt
    10981 ctgccggatg cagaaggccg tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg
    11041 ctgtgtgttg ttccgggtga aatctggggt ggtgctgttt tgcgttactt cagcgctctg
    11101 gaagaaggga tcaacctgct gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa
    11161 gaacatgatg gtcgtaagca agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc
    11221 accctgttga aaggggaaaa attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc
    11281 agttaaccat actgacgatg gtcaacatga tgggctccgg tatcatcatg ctgccgacaa
    11341 agcttgccga agttgggaca atttcaatta tctcctggct ggtgacagcc gtaggctcaa
    11401 tggcactggc atgggcattc gcaaaatgcg gtatgttcag ccgtaaatca ggcggtatgg
    11461 gcggttatgc ggagtatgct ttcggtaaat ccggtaactt tatggcgaac tatacctacg
    11521 gcgtctcgct gctgattgct aacgtcgcga ttgctatttc ggcggttggt tacggcaccg
    11581 aactgctcgg cgcgagtttg tcgccagtgc agattggtct tgcgaccatc ggggtgctgt
    11641 ggatttgtac cgtggctaac tttggtggtg cgcgcattac cgggcaaatc agtagcatta
    11701 ccgtgtgggg ggtcattatt ccggtggtag gcctgtgcat tatcggctgg ttctggttta
    11761 gcccgacgct ttacgttgat tcctggaatc cgcatcatgc accgttcttc agtgcagtag
    11821 gttcttccat cgctatgacg ctgtgggctt ttctcggtct ggagtctgcg tgtgcgaata
    11881 ctgatgtagt ggaaaacccg gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag
    11941 gtgcggcggt gatttatatc gtctccacca acgtgattgc agggattgtg cctaatatgg
    12001 agctggcaaa ttcaacggca ccatttggcc tggccttcgc acagatgttc acaccagaag
    12061 tgggtaaagt cattatggcg ctgatggtga tgtcctgctg cggttcgctg cttggctggc
    12121 agttcaccat tgcccaggtg tttaaatctt catctgatga aggctacttc cctaaaattt
    12181 tctcccgtgt aaccaaagtg gatgcaccgg tgcagggaat gttgaccatt gtgattatcc
    12241 agagcgggtt ggcgctgatg acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg
    12301 ttaacctggc cgtggtgacc aatatcattc cgtatattct gtcaatggcg gcattagtca
    12361 ttattcagaa ggtcgctaat gtgcccccct caaaagcgaa agttgcaaac tttgttgctt
    12421 ttgttggcgc gatgtatagc ttttatgcgc tgtactcatc cggggaagaa gccatgctgt
    12481 acggttccat cgtgaccttc cttggttgga cactgtatgc tctggtatta ccacgctttg
    12541 aactgaaaaa taaacacggt taaaaaagca aagtaaaaaa gggcgatctt gcgaccgccc
    12601 tttttttatt aaatgtgttt acgcgttttt cagaacttcg ctaacaatct caaccgcttc
    12661 tttctcaatc tgcttgcgat gttcttcacc gaggaagctt tcgcagtaga tcttatatgc
    12721 gtcttccgtg cctgacggac gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc
    12781 gccgatagaa gcaccattac ccggtgccgc cgtcagacgt gcggtgatcg ggtcacctgc
    12841 cagggtgctg gcgctcacca tttccggaga cagcttagac aacgccgctt tttgtgcgga
    12901 agtcgcagcg gcctgcaaac ggttgtagct cggcgcacca aagcgttttg ccagttcgtt
    12961 gtagtgttcc tgcgggttct taccggtgac agcggtgatt tccgccgcca gcagacacat
    13021 aatgatgccg tctttatcgg tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc
    13081 ggcgctctct tcgccgccaa agccgaaact gccgtcgaac agaccatcaa caaaccattt
    13141 gaatcctacc gggacttcta ccagtttacg gcccaaggcg ttgaccacac ggtcgatcat
    13201 cgcagaagaa accagcgttt taccgacagc aacatctttg ccccactgcg gacgatgctg
    13261 gaacaggtag ttgatcgcca ccgccaggta gtggttcgga ttcatcaaac ctgccggagt
    13321 gacgataccg tgacggtcat aatccgggtc gttagcaaac gccagatcga acttatcacg
    13381 cagtgccagc aggcccgcca tcgcacactc ggaggagcag tccatacgga tcgcgccgtc
    13441 tttatcaagg tgcataaagc ggaaggtttg atcgacctga tcgttaacga tggtcaggtt
    13501 gaggttgtaa tactcgccaa tacgcttcca gtattcgata ccggaaccgc ccagcggatc
    13561 aacgccaagc gtcaggcccg ctttctgaat cgccgccata tcaacgatat cggccagacc
    13621 ttccacaaac ggctgcacca gatcctgctc tttcacatga ccggatgcca tcgcttcgtc
    13681 gagagagata cgcttcacgc ctttcaggcc atcggccatc agtgcgttgg ccctgtcttc
    13741 caccactttg gtgacgttgg tatcagccgg gccaccattt ggcgggttgt acttgatacc
    13801 accatcttcc ggcgggttat gggacggtgt aatcacgata ccgtctgcca gcgggccacc
    13861 ttttttattg tgaaccagga tggcattgga aacggcaggc gtcggagtga atccattgtt
    13921 ttcctgcaca atgacatcaa cgccgttcgc tgccagcact tccagtacgg aaatgaacgc
    13981 aggctcggac agggcgtgag tatctttacc cacatagcaa gggccagtga tgccattttt
    14041 cgcacgttct tcagcaattg cctgagcgat cgccagaatg tgcggctcgt taaagctgtg
    14101 gcgcgctgca ctgccacggt gaccggaagt accgaatttc accgcgtgct ccgcattccc
    14161 tgcttctggt ttcagtacat aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact
    14221 ctgttgtgca ggttggcctg cacgattgtg gattgccatt gctttgtcct ttgtctgcaa
    14281 cgttttagat agttccgcga accttctcaa tcaattccgc cgggaattgc atcgactgca
    14341 tgatgtgttc gatcatgctg catttacggc cggtgttggt gttggtgatc acccaatacg
    14401 gcgtgcctgg cacatgtttc ggcttggtct gattaccatt tttcagcagc gtttgttcat
    14461 ctgccgcaaa gtaaacgcgt gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct
    14521 gggcgtcaag agaatatagt gtagacaaca gcagcataaa gcgattgacc gctcgctttt
    14581 gctctgcgta ttcgtccgaa agcagaagtt cacgcattgc gcgaaccttg tctttaatcg
    14641 ttttgaccgg cttcgcttcg acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg
    14701 gagcagcagg ctgtgatgag gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc
    14761 tctcgccgat atgcttagtg tggctggcaa tatagctgta gagttcatca tcaacttcaa
    14821 tcgttttcat cttaatccag tgcagtgtct tagctgaata caagtcgcca ggagtatatg
    14881 aataaatccc ggcagcggat agcgtcaagc ctggcttgca gaaaaactcg gaataaacct
    14941 tatttgttgc agccctgtgg cacaatggtc aacataatac cctaacccga caacgcgaaa
    15001 aaaagaactt tgccatgaaa ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata
    15061 attctcccat cgttcttgtc catggtctgt ttggtagcct cgacaacctt ggcgtactgg
    15121 ctcgcgatct ggtaaacgat cacaatatca tccaggttga tatgcgtaac cacggtcttt
    15181 caccgagaga tccggtaatg aattacccgg cgatggcgca ggatcttgtt gatactctgg
    15241 atgcacagca gatcgacaaa gcaacattta tcggtcactc catgggcggt aaagcggtaa
    15301 tggcacttac tgcactggcc cccgatcgca tcgataaact ggtggcgatc gatatcgcac
    15361 cggtcgacta tcacgtacgc cgtcatgatg agattttcgc ggctatcaac gcggtcagtg
    15421 aatcggacgc acaaactcgc cagcaagcag cagcaataat gcgccagcat cttaatgaag
    15481 aaggggtgat tcagtttctg ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc
    15541 cggtattgtg ggatcagtat ccgcatattg tgggttggga gaaaatcccg gcatgggatc
    15601 accctgccct gtttatccct ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg
    15661 atttactggc acaatttcca caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg
    15721 tccatgctga aaaaccggat gcggtattac gcgctatccg tcgctatctc aatgattaac
    15781 gcaactgatt aaaaacccag cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc
    15841 atcgcgctga tgtattatgg cgcgctatcc atccgggcag aggcctggct gattgtttcc
    15901 cccgaagtca ccaagatcat ggccaaagaa caaacggacc gtacgacatt agatctgttc
    15961 gcgcacgagc gtcgaccggg acgaccgaaa actaatccgc tttcgcgcga tgaacagctg
    16021 cgtattaata aacgcaacca gctaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa
    16081 ctgaagctga acgcggaagc tgtcgaggcg ctgaacgagc tggcggagtc gcgcaatatg
    16141 agccgtagcg aactgatcga agagatgtta atgcagcaac tggcggcgct gcgtagtcag
    16201 ggcatcgttt aaatttccac tttcatgtag cacagtgtgc agtcctgctc gtttgctgat
    16261 tccgcaagac tgcctgttct gctatgattg cctttatccg tgggcaattt tccaccccca
    16321 tttcaataag tttcaagagg ttatttcact catggctatc actggcatct ttttcggcag
    16381 cgacaccggt aataccgaaa atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt
    16441 tgccgatgtc catgacattg caaaaagcag caaagaagat ctggaagctt atgacattct
    16501 gctgctgggc atcccaacct ggtattacgg cgaagcgcag tgtgactggg atgacttctt
    16561 cccgactctc gaagagattg atttcaacgg caaactggtt gcgctgtttg gttgtggtga
    16621 ccaggaagat tacgccgaat atttctgcga cgcattgggc accatccgcg acatcattga
    16681 accgcgcggt gcaaccatcg ttggtcactg gccaactgcg ggctatcatt tcgaagcatc
    16741 aaaaggtctg gcagatgacg accactttgt cggtctggct atcgacgaag accgtcagcc
    16801 ggaactgacc gctgaacgtg tagaaaaatg ggttaaacag atttctgaag agttgcatct
    16861 cgacgaaatt cttaatgcct gatgtgatgc ggcgtagact catgtctacg ccgtattaat
    16921 agataatgcc aatcaaaata attgctacaa atttgtaact tttgctgttg tacctgtaca
    16981 atgtcccggt gttcaagtgg ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta
    17041 acaatatttg ccagggactt gtggttttca tttaggcgtg gcaattctat aatgatacgc
    17101 attatctcaa gagcaaattc tgtcacttct tctaatgaag tgaaccgctt agtaacagga
    17161 cagattccgc atgactgata acaataccgc cctaaagaaa gctggcctga aagtaacgct
    17221 tccacgttta aaaatcctgg aagttcttca ggagccggac aaccatcacg tcagtgcgga
    17281 agatttatac aaacgtctga tcgatatggg tgaagaaatt ggtctggcta cggtatatcg
    17341 cgtactgaac cagtttgacg acgctggtat cgtcacccgc cacaattttg aaggcggtaa
    17401 atccgtattt gaactgacac agcaacatca ccacgatcac ctgatttgcc tcgactgcgg
    17461 caaggttatc gaatttagtg atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa
    17521 acatggcatt cgcctgacta accacagtct ctatctttac ggtcactgtg ccgaaggcga
    17581 ttgccgcgaa gatgagcacg cgcacgaagg caaataagcc agcctgaacg agaaaagcca
    17641 acctgcgggt tggctttttt atacaaggga aataagaaaa gcgagagtta cagctctcac
    17701 ttatttgtta ttactgcgta tttcctgcca gaccttatca caatcggctt tcaccgcctg
    17761 atcatttccg gtttgcgttg cacgcagaca ctgctgataa tccagtacgc gtacactttc
    17821 ctggtcggca aactgctgat gttgtttctc tttcttcagc acgtttaaca cgctctggca
    17881 ggcttcaatt ttttctggcg aaccttgtgc ggtgttgata caggcgctgt atgcctcttt
    17941 cagacgactg tcttcctgtg gcgcggttga ttgcgcacaa gctaccagcc ccgatgccat
    18001 tatggcgatg agaatcagtt ttttcatagc aaattcctca aacgtgcggc aggcacaaaa
    18061 cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat ttcacgtcac
    18121 gctcatcctg gaagatgtta ccgtaaccgc cgccccagct tgggatgtca gagtggttgt
    18181 cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc tgaatggtgt
    18241 agaccgcatc caggctgtag gcagactctt caatagtccg gtttttgtcg tagtacgcat
    18301 ccggattgct ctgccaggtc gcaggtttag catcccatgc gtaaacgtag gaagcgccga
    18361 tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag aagaccgctt
    18421 tttcgccgtt ggcgttgaag tcggaacggt tatcccacca gatatccagg cggccgttgg
    18481 aggaagcgta ggttggagtc atacgttgca ggaagtatcc ctgctgaccg tcagccttaa
    18541 cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca aaggtcaacg
    18601 cttgcagcca ggcggtgccg tcataaaggt cgttgacgct gcgatcgtca actttatcgc
    18661 gcgtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca aatttgtagc
    18721 tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc gcttccagta
    18781 cgaagttatt tttgaagtcg tatttcgcac cgatggagtg caggtaatca actttggtgg
    18841 ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg tattcgttgg
    18901 tccacatgta ggagaaactc aaggcaccag catcgccgta atcaaaattc gccccggctt
    18961 ccgccccctg ataagtacct ggcataaagc tccagtgcgg cgctaacagc gtttgaccag
    19021 ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg gccgcagctt
    19081 tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct ttattacttt
    19141 ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt tcaatcgccg
    19201 taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga aaatcgaggt
    19261 tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg gtaacatctt
    19321 tacggtcgcg ttcacgctgc cagtaatata taccgccggt taaggttgaa tcgtcgatga
    19381 atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta acaccggcga
    19441 tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa tcctctttga
    19501 cgtataaatt gctgcaccaa aggtgaacag cgaaaaaaat aaaaattaaa aaagtagcgt
    19561 tgagaaaagt ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct
    19621 ctttctgtaa ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta
    19681 caaggcttaa gtttgcggtt gaaaggcttg cagaagaata aaagcgtgaa tttcca
//
LOCUS       DAZGTR010000081        19706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGTR010000081 DAZGTR010000000
VERSION     DAZGTR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.081"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(390..766)
                     /gene="rnpB"
                     /locus_tag="ACHLCY_003220"
     ncRNA           complement(390..766)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHLCY_003220"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(799..1944)
                     /gene="garK"
                     /locus_tag="ACHLCY_003221"
     CDS             complement(799..1944)
                     /gene="garK"
                     /locus_tag="ACHLCY_003221"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP0370422.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2041..2931)
                     /gene="garR"
                     /locus_tag="ACHLCY_003222"
     CDS             complement(2041..2931)
                     /gene="garR"
                     /locus_tag="ACHLCY_003222"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0370423.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2961..3731)
                     /gene="garL"
                     /locus_tag="ACHLCY_003223"
     CDS             complement(2961..3731)
                     /gene="garL"
                     /locus_tag="ACHLCY_003223"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP0370424.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATKKLADTFKK"
     gene            complement(3747..5081)
                     /gene="garP"
                     /locus_tag="ACHLCY_003224"
     CDS             complement(3747..5081)
                     /gene="garP"
                     /locus_tag="ACHLCY_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP0370425.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5456..7027
                     /gene="garD"
                     /locus_tag="ACHLCY_003225"
     CDS             5456..7027
                     /gene="garD"
                     /locus_tag="ACHLCY_003225"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP0370426.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7176..7511
                     /gene="prlF"
                     /locus_tag="ACHLCY_003226"
     CDS             7176..7511
                     /gene="prlF"
                     /locus_tag="ACHLCY_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP0370427.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7511..7975
                     /gene="yhaV"
                     /locus_tag="ACHLCY_003227"
     CDS             7511..7975
                     /gene="yhaV"
                     /locus_tag="ACHLCY_003227"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP0370428.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8030..8839)
                     /gene="agaR"
                     /locus_tag="ACHLCY_003228"
     CDS             complement(8030..8839)
                     /gene="agaR"
                     /locus_tag="ACHLCY_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP0370429.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9088..10368
                     /gene="kbaZ"
                     /locus_tag="ACHLCY_003229"
     CDS             9088..10368
                     /gene="kbaZ"
                     /locus_tag="ACHLCY_003229"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP0370430.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10391..10864
                     /gene="agaV"
                     /locus_tag="ACHLCY_003230"
     CDS             10391..10864
                     /gene="agaV"
                     /locus_tag="ACHLCY_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP0370431.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10875..11654
                     /gene="agaW"
                     /locus_tag="ACHLCY_003231"
     CDS             10875..11654
                     /gene="agaW"
                     /locus_tag="ACHLCY_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP0370432.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11644..12522
                     /gene="agaE"
                     /locus_tag="ACHLCY_003232"
     CDS             11644..12522
                     /gene="agaE"
                     /locus_tag="ACHLCY_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP0370433.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12540..12974
                     /gene="agaF"
                     /locus_tag="ACHLCY_003233"
     CDS             12540..12974
                     /gene="agaF"
                     /locus_tag="ACHLCY_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP0370434.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLALQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12971..14104
                     /gene="nagA"
                     /locus_tag="ACHLCY_003234"
     CDS             12971..14104
                     /gene="nagA"
                     /locus_tag="ACHLCY_003234"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0370435.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14455..15609
                     /locus_tag="ACHLCY_003235"
     CDS             14455..15609
                     /locus_tag="ACHLCY_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP0370436.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15622..16482
                     /gene="kbaY"
                     /locus_tag="ACHLCY_003236"
     CDS             15622..16482
                     /gene="kbaY"
                     /locus_tag="ACHLCY_003236"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP0370437.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16649..17125
                     /gene="agaB"
                     /locus_tag="ACHLCY_003237"
     CDS             16649..17125
                     /gene="agaB"
                     /locus_tag="ACHLCY_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP0370438.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17164..17967
                     /gene="agaC"
                     /locus_tag="ACHLCY_003238"
     CDS             17164..17967
                     /gene="agaC"
                     /locus_tag="ACHLCY_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP0370439.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17957..18748
                     /gene="agaD"
                     /locus_tag="ACHLCY_003239"
     CDS             17957..18748
                     /gene="agaD"
                     /locus_tag="ACHLCY_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP0370440.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18749..19504
                     /locus_tag="ACHLCY_003240"
     CDS             18749..19504
                     /locus_tag="ACHLCY_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP0370441.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGLTLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4785 a         5137 c         4996 g         4788 t
ORIGIN      
        1 ataacttctt acaaagtcta ttattaatac cgtagatatt tattcatctt accctgtgca
       61 ccaaaagcaa aaactcactg acgcgggttt gaaactgatg gcaaccgcaa aaatgcctga
      121 tgcgctacgc ttatcaggcc tacgccatct ctgcaatata ttgaatttgc gtgcttttgt
      181 aggcaggata aggcgttcac gccgcatccg gcatgaataa agcgcactca acaatctgaa
      241 acccgccgga acggtttatt acgtacatca ggtgaaactg accgataagc cgctttcttt
      301 tgggtatagc gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc
      361 ggtttacgct ggcgggtttt cgttaaatca ggtgaaactg accgataagc cgggttctgt
      421 cgtggacagt cattcatcta ggccagcaat cgctcactgg ctcaagcagc ctacccgggt
      481 tcagtacggg ccgtacctta tgaaccccta tttggccttg ctccgggtgg agtttaccgt
      541 gccacggact gttaccagcc gcgcggtgcg ctcttaccgc accctttcac ccttacctga
      601 tcccgcttgc gcgggccatc ggcggtttgc tctctgttgc actggtcgtg ggtttccccc
      661 ccaggcgtta cctggcaccc tgccctatgg agcccggact ttcctcccct ccacccgtct
      721 cccccgaaga ggacgacgac gaagcggcga ctgtctggtc agcttcggcg cgcagtatag
      781 agggtttgcg cgcccttgtc accccgcgtt gcgcattcca atcgccagtg tcgcggcgat
      841 attacgtgaa gcacggcaga tattgtcata agccccgcgg aatgcttcgt ccaacgtacc
      901 aatgctggtc agtacgctga agacagcatc aatgccatgc tgatgtacaa cgccaacatc
      961 atcggtcagg ctacccgcaa tgccaatcac cggtttatgg tacttcttcg ccacgtttgc
     1021 gacgccaatc ggtaccttgc cgtgaatact ctggctgtca atacgccctt caccggtgat
     1081 caccagcgta caatcgtgaa tatgctcctc cagattcagc gccgtagtga cgatttcaat
     1141 accacttttc agttccgcac caagaaacgc cattagcgcc gcgcccatac cacccgcagc
     1201 tcctgcgccg gggacatctt tcacatcaac atgcagcgct tttttaatga cctcggcata
     1261 gtgagagagg ttattgtcca gctcaacaat catcgcttca ctggctccct tttgtgggcc
     1321 aaagatgcgc gatgcgccgt tatcgcccac cagcggattg gtgacatcac aagcgacgcg
     1381 aatgacgcaa tcttttaagc gcggatcgag gccggaaata tcaatatcat tcagagtatt
     1441 aagactaccg ccgccaaaac caatttcatt gccgttggcg tcgcataatt tcgcccccag
     1501 cgcctgtacc atgcctgcgc cgccatcatt tgtagcgcta ccgccaatgc cgataataat
     1561 gtttgtcgcg ccgctctcca gcgcctgcag gattaactcg cctgtgccgc gtgaagtggt
     1621 cacgagtgga tcgcgttttt ccgcaggtac cagctccagc ccactggccg ccgccatttc
     1681 aataaacgcg gttttgccat cgccggagat cccccaactg gcattcactt tctcgcccag
     1741 cggccctgta acccaggcgt gacgttcaga accctgggtg gctgcaatca tcgcttccac
     1801 cgttccttcg ccaccgtcgg caaccggaat agaaacgtac tgtgcatcag gaaaaatttc
     1861 ccgaaatcct ttttctatcg cctgcgcaac ctcgctggca gataaacttt ctttataaga
     1921 gtctggggcg attacgattt tcatacctat gcctgttacc acatgacgcc ggagggcgtt
     1981 tctcttattc ggcctggatt ccaggcccgg attgcaatac gccatccggg cacgacgtca
     2041 ttaacgagta acttcgactt tcgccagttt ttcgtagtag cacgccaggg cgctatgatc
     2101 cgccgttcct aaaccatctg ctcgcagtgc ctgcatcatc tccataaccg cagctgtgag
     2161 cggcagttgc gcgccgacgc cgtgggaagt atccagcgca tttgccagat ccttaatatg
     2221 cagatcaata cggaagcccg gcttgaagtt gcggtccatc accatcggcg ctttggcatc
     2281 cagcacggta ctgcccgcca gtccaccgcg aattgcctga tacaccaggt ccgggttaac
     2341 gcccgctttg gttgccagcg ttagcgcttc tgacatcgcg gcaatattca gcgccacaat
     2401 gacctgattt gccagtttgg tgacgttacc tgcaccgatt tccccggtat gcaccacgga
     2461 acccgccatc gctttcatca aatcatagta tttgtcgaaa atagccttgt cgccgcccac
     2521 catcactgac agcgtaccgt cgatggcttt cggttcaccg ccgctcaccg gagcatccag
     2581 catatcaatg cctttcgctt tcagcgcttc gctgatttca cggcttgcca gcggtgcgat
     2641 agaactcata tcgatcaata ccgtacctgg cttcgcgcct tcaataatgc cattctcacc
     2701 cagcgccacc tctttcacat gaggggagtt tggcagcatg gttatgatga cgtcgcactg
     2761 ttcagcgatc gctttagccg tagacgctgt ttctgcacct gcagcaatca cgtcagcaat
     2821 agcttctggg ttacggtcag caaccaccag cgagtaacct gctttcagaa ggtttttact
     2881 cattggttta cccataatcc ccaggccaat aaaaccaact ttcatagtca tatcaatcat
     2941 ctctcttgtt gcggtggtgg ttatttttta aaggtatcag ccagtttctt agtggcagag
     3001 cggaagacgc caagatcgct gccgacagcc acaaacgtcg caccccattc cagataacga
     3061 cgcgcatcgg cttcgaccgg cgcgagaata ccgctgggtt tgccgtgcgc actggcacgg
     3121 ttaaaaatgt gctgaattgc tttttgtaca tccgggtgcg aggcattgcc gagatggcct
     3181 aatgccgcag ccagatcgct ggggccgacg aagataccgt ctacaccttc ggtagcggca
     3241 atggcatcga cgttatctac gccctgctga ctttctatct ggaccagaat ggtgatgttc
     3301 ttgttcgact gagcgaaata atccgccacg gtgccaaaca tattggcgcg gtgagaaacg
     3361 gagacgccgc gaatgccttc cggtgggtaa cgggttgatg ccaccgcctg ctctgcttcc
     3421 tcttttgttt ctacaaaagg aatcaggaag ttatagaaac cgatatccag aagacgctta
     3481 ataattaccg gctcgttggt cggcactcgc actactggcg cgctggcgct gcctttcagg
     3541 gccattaact gcggaataaa cgtggagata tcgtttggcg catgttcgcc atccagcacc
     3601 agccagtcaa acccagccaa accaagaact tcagtgctaa tcgggttaga gagtgctgac
     3661 cagcaaccaa tttgtacctg tttcgcagcc agtgcggctt tgaatttatt cgggaaaaca
     3721 tcgttattca tcgcttatac ctttgcttat ttctgcaatt ccatacgttt aatgtcgcca
     3781 actacgaaga ggtagcagac catcgccatc agcgctgaac atcccacgaa aaccagtgct
     3841 gcattgaagg agtgcagttc acttaccagg tagccaatca ccagtggagt gacaatggag
     3901 gcaacattgc caaagacgtt aaagacgccg ccacagaggc caacaatctc tttcggcgcg
     3961 gtgtcagaaa tcaccggcca gcccagagca ccaaatcctt tgccaaaaaa agccagcgcc
     4021 atcagcatga ccaccagcgt ggtgttgttg gtgtagttac ataagatgat ggtggaagcc
     4081 agcaacattc ccagcacaat cggtagctta cgtgccaggg tcagggataa accgcgtttg
     4141 atcagataat ccgagaagac acctcccagc acgccgcccg caaaaccaca cagtgccgga
     4201 atcgaggcga ccagacccac tttcagaatc gacatgcctt tttcctgcac cagataaatc
     4261 gggaaccagg tgaggaagaa ccaggtgatg gtgttgataa aatattgtcc gaaaaatacg
     4321 cccaacatca tgcggttaga gagcaattgc ttgatgtaat gcagtttggg accgcttgct
     4381 gccgcgctgc ccggcttttt gtggtccata tcgaccaccg cgccattttc agagataaac
     4441 ttcagctctt ccgcagacat acgtgggtga tctgtcgggt tatgaatcaa tttgatccac
     4501 agcgccgtca gcacaaaacc aatcaccccc ataacggtaa agacgtgctc ccagccccag
     4561 gcgaaagtca gccagccaag cagaggcgaa aagagcgcca gcgagaaata ttgcgccgag
     4621 ttaaagatgg cggaggcagt accacgttct ttcgtcggga accaggcggc gacaatacgg
     4681 gcgttcgccg ggaatgatgg cgcttccgag aagccgagca taaagcgcat aaagaacatg
     4741 gagatccctg cccaggccag cgggaacata tcaacaaagc cttgcaggaa agtgaatagc
     4801 gaccagaaaa agaggctata ggtataaact tttttcgagc caaacttatc aagcagccag
     4861 ccgccgggga tttgcatcag caagtaggcc cagccaaaag cagaaaagat gtaacccatc
     4921 gaaaccgcac ttaactgcaa ctcttttgcc acttcggtac cagcaataga cagcgttgca
     4981 cgatcggcgt agttaacggc ggtaacaata aaaataatca gtaatattaa atagcgggta
     5041 tgcacgcctt tctttttttc gtcaacggtg tccagaatca ttttatttac ctcgggtact
     5101 tatgctgatt tttattatta tggggaaggt gttatttatg agtttcattt atgccgtaac
     5161 ggcaatgaac tcgggaatta gtataagcag cgcgagaata ataatcattg tgcaaatgct
     5221 aatttaatta atgctattta aatattattt tgagcatatg cacataaggt tgcgcgctaa
     5281 agcacagatt tgcgctttac cttgccgggc ggcactgcaa tccctgaaat gattgacatt
     5341 gatcacattt ctgcgtttaa actcctgaca ttcttatttc acccaatgaa gtcatttatt
     5401 tttaaatgag accaggtcct cattttaata acccctggct ggagaatata gtacaatggc
     5461 caacatcgaa atcagacaag aaacgccaac tgcgttttat ataaaagttc acgacacaga
     5521 taatgtggca attattgtta atgataatgg cctgaaagca ggaacgcgtt ttccggatgg
     5581 actggaatta attgaacata ttccccaggg gcataaagtc gcattgctgg acattccggc
     5641 taatggtgaa attattcgtt atggtgaagt gattggttac gccgtgcgtg caatcccacg
     5701 cggaagctgg atcgacgaat caatggttgt actaccggaa gcgccgccgt tacacacgct
     5761 gccactggca accaaagtcc cggccccctt accgccgctg gaaggataca cctttgaggg
     5821 ctatcgcaat gccgatggca gcgtgggcac caaaaacctg ctcggcatca ccaccagcgt
     5881 ccactgtgtg gcaggcgtgg tggactatgt agtaaaaatc attgaacgcg atctgctacc
     5941 gaaatacccg aacgtcgatg gcgtggtggg gctgaatcat ttgtacggct gtggcgtggc
     6001 gattaacgca ccggcggcag ttgtgcctat tcgtaccatc cacaatattt cgctgaaccc
     6061 taactttggc ggcgaagtaa tggtgattgg tctgggttgt gaaaagttgc agcctgagcg
     6121 cctgctggtc ggcacggatg atgtgcaagc tattccagta gaaagcgcca gcattgtcag
     6181 tttgcaggat gaaaagcatg tcggttttca gtccatggtc gaggatattt tgcaggtcgc
     6241 cgaacgccat ctacaaaaac tgaatcaacg tcagcgagaa acctgtccgg cttcagaact
     6301 ggttgtcggc atgcagtgcg gtggcagcga tgccttttct ggcgtaacgg caaacccggc
     6361 ggttggctat gcgtctgatc tactggtgcg ctgcggcgca acggtgatgt tctcagaagt
     6421 cacggaagtg cgtgacgcga tccatctgct gacaccacgc gcagtgaacg aagaggtcgg
     6481 caaacggctg ctggaggaga tggagtggta cgataactat ctcaatatgg gaaaaaccga
     6541 ccgcagcgcc aacccttcgc cgggcaacaa gaaaggcggt ctggcaaacg tggtagagaa
     6601 ggcactcggc tccattgcta aatcgggtaa aagcgcaatt gttgaagtgc tgtcgcccgg
     6661 tcaacgcccg actaaacgcg gattaattta cgccgcgacg ccagccagcg attttgtctg
     6721 tggcacgcaa caggtggctt cgggtatcac cgtgcaagtg tttacgaccg gtcgtggtac
     6781 gccgtacggc ctgatggcgg tacccgtcat taaaatggcg acccgcaccg agctggcgaa
     6841 ccgctggttt gatttaatgg atattaacgc gggcactatc gccaccggcg aagaaaccat
     6901 tgaagaggtg ggctggaagt tgttccactt tattctcgac gtcgccagcg ggaagaagaa
     6961 aaccttctcg gatcaatggg ggctgcataa ccagctggca gtgtttaacc cggcaccggt
     7021 gacctgattt cttttggaac gtccctcgca aaacatggcc ttagtgccat gtttttattg
     7081 tttaaagccc ccacgtccat taataatgca tttgcattac cttagttcaa gcttataatt
     7141 tgagcagaaa acaggctgta aaaggacagt gaatcatgcc cgctaatgct cgctctcacg
     7201 ctgtactgac cactgaatca aaggtcacga tacgcggaca aacaactatc cccgcaccag
     7261 tgcgtgaggc cttaaaactg aagccaggcc aggacagcat tcattacgaa attctgcctg
     7321 gtgggcaagt ctttatgtgc cgactgggag atgaacagga ggatcatact atgaatgcat
     7381 ttttgcgttt tctggatgca gatatccaga acaacccgca aaaaactcgt ccattcaaca
     7441 ttcaacaagg aaagaaactt gtcgctggca tggacgtcaa cattgatgat gagattggcg
     7501 acgacgaata atggattttc cacaaagggt taatggttgg gcgctatatg ctcatccctg
     7561 ttttcaggaa acctacgacg ctttagttgc cgaagtcgag acattaaagg gaaaagatcc
     7621 tgaaaattat cagagaaaag ccgccacaaa gttattggcg gtagtccata aagtgattga
     7681 ggagcatatc acggtcaatc catcatcacc ggcattccgt catggcaagt cgttaggctc
     7741 tgggaaaaat aaagactggt cacgggtaaa atttggtgct ggtcgttatc gtctcttctt
     7801 tcgttatagt gaaaaagaga aagtcatcat tctgggatgg atgaacgatg aaaacactct
     7861 gcgcacctac ggtaaaaaaa cagatgccta taccgtattc agcaaaatgt taaaaagagg
     7921 acatcctcct gccgactggg aaaccctcac ccgagaaaca gaagaaaccc attgatggtg
     7981 ttcacaatgc ccgccgaacg tattcgtgtt gaccagcggg caccctttct cactccccga
     8041 ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca ggaatgcctt
     8101 catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat
     8161 tgaatttact ggaatcggtg accacgatga tccgttccac aacttcgcac atccgacggt
     8221 ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta
     8281 caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt
     8341 aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg
     8401 cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtgct
     8461 tacgcatcag acgagcaatc tcaaaagtgg tagtcccgga atcgaggatc acccgatgac
     8521 caggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg gtgttcagcg
     8581 cgcttttatc ttccactgat ggctcgactg acggcgtcgt gctatcgcag atcaacgcgc
     8641 caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatca ttgcggatcg
     8701 tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc
     8761 gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc agtcactcgc ttctcacctg
     8821 aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc
     8881 ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattgac gcaagctact
     8941 ctcgtttcag tgactttcat tatgtttctt ttgtgaatca gatcagaaaa ctattatctt
     9001 tcgttttatt tttatctcac catgacgcag tatcaactga aacaaaacga aagattaata
     9061 tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac
     9121 aaagcgggca aaacaaatgg aatttatgcc gtttgttccg cacatccgct ggtgctggag
     9181 gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat
     9241 caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt
     9301 tgtcagctcg ccgactcgtt gaatttcccg caggatgcgt tgattctggg tggtgaccat
     9361 ctggggccaa accgctggca aaacctgccg gccgctcagg caatggccaa tgccgatgat
     9421 ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc
     9481 tgtcaggacg atccgattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg
     9541 gcgaaagtgg cggaagaaac ctgtcttgaa cactttggcg aagccgatct ggagtatgtc
     9601 attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc
     9661 accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa
     9721 ggtttgaatg ccatctggcc acgcatcatt gccctggtgg ttcaacccgg cgtcgaattc
     9781 gatcacacca acgttattga ttatcagccc gccaaagcga gcgccttaag ccagatggtc
     9841 gaaaactacg aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatcg
     9901 ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccagc gctgaccttc
     9961 gccctgcgtg aagctctgtt ctctctggcg gcgattgaag aagaactggt gccagcgaaa
    10021 gcctgttctg gtctgcgtca ggtgctggaa gacgtgatgc tcgaccgccc ggaatactgg
    10081 caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta tagctactcg
    10141 gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc tcatctggtt
    10201 cgtaatctgg cggattcacc aattccgcta ccgctcatca gccagtatct gccgctgcaa
    10261 tacgtgaaag ttcgctccgg cgagctgcaa ccgacgccac gggaactcat tatcaaccat
    10321 attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc aaaacaaaga
    10381 ggaacacgct atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca
    10441 ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag ccaacgatga
    10501 ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc
    10561 cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca
    10621 gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt
    10681 tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaaac aacaaatcgc
    10741 caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg
    10801 ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct gtagacctct ttaaattact
    10861 ttgagggatt catcatggaa atcagcctgt tgcaggcatt tgcgttgggc attatcgcct
    10921 ttatcgctgg cctggatatg tttaacggcc taacacatat gcaccgcccg gtggtcctcg
    10981 gcccgttggt cgggctggta cttggcgatc tgcataccgg aattttaacc ggcggtacgc
    11041 tggaactggt gtggatgggg ctggccccgc tggcgggcgc acagccgcct aacgtgatta
    11101 tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcgg
    11161 tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg
    11221 tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca
    11281 ttgaacgcgt gaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt
    11341 tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac
    11401 gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc
    11461 tgctgaaaat catgatgaaa aacgtctaca tcccctactt catcctgggc tttgttgccg
    11521 cagcctggct caagttaccg gtgctggcta ttgctgccgc tgcgctggcg atggcgctga
    11581 tcgacctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg
    11641 aagatggcat ctaatcaaac caccctgccg aacgtcagtg aaaacgaaga aacattgctg
    11701 actggcgtca atgaaaacgt gtatgaagat cagagcattg gcgcggagct gacgaaaaaa
    11761 gatatcaacc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt
    11821 atgcaggctt ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat
    11881 aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt
    11941 ctggtgacat ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac
    12001 agtattcaga gcaccaaaat tgccgtcggt gcgccgctcg gcgggattgg cgatgcaatg
    12061 ttctggctaa cgctactgcc gatttgtggc gggattggag ccagtctggc attgcaaggc
    12121 tccattcttg gcgcagtcgt ctttattctg ctgttcaacg tggtacacct ggggctgcgt
    12181 tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattccact cattaaagcg
    12241 aataccaaaa aagtcggcca tgctgcgtct atcgttggga tgacggtaat cggtgcgctg
    12301 gtggcaacct atgttcgttt aagcaccacg ctggaaatca cagcgggcga cgcagtggtt
    12361 aagttacagg ctgatgttat cgacaaactg atgcctgcct tcttaccgtt ggtctacacc
    12421 ctgaccatgt tttggttggt acgccgcggc tggagtccgc tgcgtctgat tgcagttacc
    12481 gtggttctcg gcatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga
    12541 tgttaagtat tattttgaca gggcatggcg ggtttgccag cggcatggaa aaagcaatga
    12601 agcaaattct cggtgagcaa tcgcagttta tcgccatcga ttttccggaa acctcaagca
    12661 ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca
    12721 ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcgc
    12781 tgcaaaaacc gggctgtgaa gtgatcaccg gcaccaattt gcaactgcta ctggagatgg
    12841 tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac
    12901 atcgtgggct gaccagtctg gtggacgagt taggccgctg ccatgaagaa tgtccggtcg
    12961 aggaagggat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggct
    13021 cgatgaccat cagttgcgta ttgctgacgg tgtcatcgca gcaatcgaac cgattccagt
    13081 gggcgtgact gaacgcgatg cggaactgct ctgccccgct tacatcgaca cccatgtaca
    13141 cggtggtgcg ggcgttgatg taatggatga cgcgccggat gtactcgaca agctggcaat
    13201 gcacaaggca cgcgaaggtg tcggcagttg gttgccgact accgtaaccg cgccgcttaa
    13261 caccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc
    13321 gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca
    13381 tccgccggaa ttgtttcgcg agcttgaaat tgccgagctg gatcaattga ttgccgtttc
    13441 tcagcacacc ttacgcgtgg tagcgctggc accggaaaaa gagggggcat tgcaggccat
    13501 ccgccatctt aaacagcaaa atgtacgagt gatgctgggg catagcgcgg cgacctggca
    13561 acaaactcgc gccgcgtttg atgctggtgc cgacggcctg gtgcattgct ataacgggat
    13621 gacaggttta catcaccgcg aaccgggaat ggttggcgcg ggattaacgg acaagcgcgc
    13681 ctggctggaa ctgatagccg atggtcatca tgtgcatccg gcggcgatgt cgctgtgttg
    13741 ttgctgtgca aaagagagaa tcgtgctgat caccgacgcg atgcaggcag ccgggatgcc
    13801 ggatggtcgc tatacgttat gtggcgaaga agtgcagatg cacggtggcg ttgtccgtac
    13861 cgcgtccggt gggctggcgg gcagtacgct gtctgttgat gcggcagtgc gcaacatggt
    13921 cgagttgacg ggcgtaacgc ctgcggaagc cattcatatg gcatcgctgc atccggcgcg
    13981 aatgctgggt gttgatggtg ttctgggatc gcttaaaccg ggcaaacgcg ccagcatcgt
    14041 tgcgctggat agcgggctgc atgtgcaaca aatctggatt cagagtcaat tagcttcgtt
    14101 ttgatatttt gctcctttat tgggccttca cttcccccgt aaggcctttc tttttctttc
    14161 gttttgatct gtgcagcggt gtcggatgcg acgctaacgc gtcttatgcg acctacggta
    14221 cgccgcatcc agcaatcggt gaacatagcg acgtcattac gcaattcttt aatctcccct
    14281 tgtcttttct ttcaatgatc acagatttcg ttttatttcc tttttctcca ttgaactttc
    14341 ggtttctttt ctatagattt taatcaacga aagacatcac caagtgaaat gaaacgaaag
    14401 gcaagtgaaa gcgacaacgc ccgacgtcaa gttcatcaga ctaaggattg agttatgcca
    14461 gaaaattaca cccctgctgc cgccgcaacc ggtacatgga ctgaagaaga gatccgccat
    14521 cagcctcgcg catggatccg ttcactcacc aacatcgacg cgctacgttc tgcgctcaat
    14581 aacttccttg aaccgttact gcgcaaagag aatctgcgga tcatcctgac cggagccgga
    14641 acctcggcat ttatcggtga catcatcgcg ccgtggctcg ccagccatac cggtaaaaac
    14701 ttcagcgccg taccgaccac cgatctggtc accaatccga tggactacct gaacccagct
    14761 catccgctgc tgttgatctc cttcggtcga tccggcaaca gcccggaaag cgtcgctgcc
    14821 gtggaactgg caaatcaatt tgtaccagaa tgctatcacc tgccgatcac ctgcaacgaa
    14881 gcgggcgctc tttaccaaaa cgcgatcaac agcgataacg cgtttgccct gctgatgccc
    14941 gcagaaacgc acgatcgcgg ctttgcgatg accagcagca ttaccaccat gatggccagc
    15001 tgcctcgcgg ttttcgcacc tgagacgatc aacagccaaa ccttccgcga cgtggcggat
    15061 cgttgccagg cgatcctgac ctcactgggc gatttcagcg aaggtgtgtt tggttacgca
    15121 ccgtggaaac ggatcgttta tctcggcagc ggtggcttac agggcgcagc acgcgagtcg
    15181 gcgctgaaag tgctggaact gactgcgggt aaactggcgg ccttctatga ttccccgacc
    15241 ggattccgtc atggcccgaa atcactggtc gataacgaaa cgctggtggt ggtatttgtc
    15301 tcaagccacc cttacacccg tcagtatgat cttgatctgc tggctgaact ccgccgtgac
    15361 aaccaggcaa tgcgcgtaat cgccatcgcc gcggaaagca ccgacatcgt cgctgccggt
    15421 ccacatatta tcctgccgcc gtcacgtcac tttatcgacg ttgagcaggc attttgcttc
    15481 ctgatgtacg cccagacgtt tgcactgatg cagtcgctgc acatgggcaa tacgccggat
    15541 accccatcag ccagcggcac cgttaaccgc gtggtgcaag gcgtaatcat tcatccgtgg
    15601 caggcataag aggatcgcat tatgagcatt atctccacta aatatctgtt acaggacgcc
    15661 caggccaatg gctacgcggt gcctgctttt aacattcata acgccgagac gatccaggcg
    15721 atccttgaag tgtgcagtga aatgcgatcg ccggtgatcc tcgccggaac gccggggacc
    15781 tttaaacata tcgcgctgga agagatctac gccctgtgca gcgcctattc cacaacctac
    15841 aacatgccac tggcgctgca tctcgaccac cacgaatcgc tggatgatat tcgccgtaaa
    15901 gtccacgcag gtgtgcgcag tgcaatgatc gacggcagcc acttcccgtt tgccgagaac
    15961 gtgaagctgg tgaaatcggt tgttgacttc tgccactcac aggattgcag cgtggaagca
    16021 gaactgggcc gcctgggcgg tgttgaagat gacatgagcg ttgacgccga aagtgcattc
    16081 ctgaccgatc cacaagaagc taaacgcttt gtcgaactga ctggcgtcga cagcctggcg
    16141 gtagcgattg gtacggcgca cggcttatac agcaaaacgc cgaagattga tttccagcgg
    16201 ctggcggaaa ttcgtgaagt ggtggatgtt cctctggtgc tacatggtgc cagcgatgtt
    16261 ccggatgaat ttgtccgtcg cactattgaa cttggcgtca caaaagtgaa cgttgccaca
    16321 gaattaaaaa tagccttcgc tggcgcggtt aaagcctggt ttgcggaaaa tccgcagggt
    16381 aatgatcctc gttattatat gcgcgtcgga atggatgcga tgaaagaagt tgtcagaaat
    16441 aaaattaatg tctgtggttc agcgaatcga atttcagcat aatcattagt tttttatgat
    16501 ttatcccaat gtacttcccg attaatatcg gggagtgcct taatggaaaa ggagatgact
    16561 aaaccttaat aaatacatca gtacaatatc gcaacaataa tatattaaaa aaaattatat
    16621 tattcaactt tatggtgagg attacacaat gagcagtcca aatattctct taacccgtat
    16681 tgataaccgt ctggttcacg gtcaggttgg cgtgacctgg acatccacca tcggtgcgaa
    16741 tctgctggta gtcgtggatg atgttgtcgc taacgatgat attcaacaga aattaatggg
    16801 tattaccgca gaaacctacg gctttggcat tcgtttcttt actatcgaaa aaaccattaa
    16861 cgtcatcggc aaagctgcac cgcatcagaa gatcttcctg atttgccgta cgccacaaac
    16921 ggtacgtaaa ttggtagaag gtggcattga cctgaaagat atcaacgtcg gcaatatgca
    16981 tttctcggaa gggaaaaagc aaatcagcag taaagtttat gtcgatgacc aggatctcgc
    17041 ggacttacgt tttattaaac aacgtggcgt gaatgttttc attcaggacg tccctggcga
    17101 tcaaaaagaa caaatccctg actaaatcta aaatcgcctt aatattggtt tgaggtaata
    17161 aaaatgcatg aaataaccct acttcaggga ttatccctgg cggcgttagt tttcgttctg
    17221 gggattgatt tttggctgga agccttattt ttattccgcc cgataatcgt ttgtacccta
    17281 actggcgcta ttctcggtga tattcagact ggcttaatta ccggtggtct gacagagttg
    17341 gctttcgccg gattaacccc tgcaggtggt gttcagccgc ccaacccgat tatggcgggt
    17401 ctgatgacca ccgtcattgc atggtctacg ggcgttgatg ccaaaacagc aattggtctt
    17461 ggcctgccgt ttagtttgtt aatgcagtac gtcattctgt tcttctattc cgctttctca
    17521 ttatttatga ccaaagccga taaatgcgcg aaagaggcgg atacggcagc gttttcccgg
    17581 cttaactgga caacgatgct catcgtcgct tcagcgtatg cggtgattgc tttcctctgt
    17641 acttacctgg cacaaggggc gatgcaggcg ctggtgaaag cgatgcccgc ctggttgacc
    17701 cacggctttg aagtggctgg cggtattctg cctgccgttg gttttggctt gctgctgcgc
    17761 gtaatgttca aagcgcaata tatcccttac ctgatcgccg gtttcctgtt tgtttgctac
    17821 atccaggtca gcaacctgtt gccggttgcc gtactgggcg caggctttgc ggtgtatgag
    17881 tttttcaatg cgaaatcccg gcagcaagcg caaccgcagc ccgttgccag taaaaatgaa
    17941 gaagaggact acagcaatgg gatctgaaat cagtaaaaaa gatatcaccc gtctgggctt
    18001 tcgttcgtcg ctgctgcaag cgagctttaa ctacgaaagg atgcaggcgg gcggttttac
    18061 ctgggcgatg ttgccgatcc tgaaaaagat ttataaggac gacaaaccgg gcttaagcgc
    18121 ggcgatgaaa gataacctcg aatttattaa tacccacccg aatctggtcg gattcctgat
    18181 ggggttatta atttcgatgg aagaaaaagg agaaaaccgc gacaccatta aaggcctcaa
    18241 agtggcactg tttggcccaa tcgccgggat tggcgatgcg attttctggt ttactttgtt
    18301 gccgattatg gcgggaattt gctcatcatt tgccagccag ggaaacctgc tggggccgat
    18361 tctatttttc gccgtttacc tgcttatctt tttcctgcgc gtcggctgga cccacgtcgg
    18421 ttattcagtc ggcgtgaagg cgatcgataa agtgcgagag aactcgcaga tgattgctcg
    18481 ctcggcaacc atcctcggga tcacggtaat cggcgggctg atcgcttcgt atgtgcatat
    18541 taacgtggtg acatcgtttg ccatcgacag tacccacagc gtcgcgctgc aacaggattt
    18601 cttcgataaa gtcttcccga acattttacc gatggcctac accctgctga tgtattactt
    18661 cctgcgggtg aaaaaagcgc atccggtgct gttaatcggc gtgacttttg tgctctctat
    18721 tgtttgttcc gcattcggca ttttgtaaat ggaacgaggc actgcttctg gcggtgcctc
    18781 tttacttaag gaatttcatc ctgtgcaaac ccttcagcaa gttgaaaact atacggcgtt
    18841 aagtgaacga gccagcgaat atttattggc tgtgatccgt agcaaaccgg atgccgtgat
    18901 ttgcctggcg accggagcca cgccattact gacgtatcat tatctggtag aaaaaatcca
    18961 ccagcagcag gttgatatca gccagctcac cttcgtgaag ctcgacgaat gggtggatct
    19021 gccattaacg atgcctggca cctgcgaaac tttcctacaa cagcatatcg tgcagccgct
    19081 ggggctgcgt gaagaccagc tcatcagctt tcgctccgaa gagataaatg agacagagtg
    19141 cgaacgggta acgaacctga ttgcgcgcaa aggcggtctg gatttatgcg ttctaggatt
    19201 ggggaaaaac ggtcatcttg ggctgaacga accgggagaa agcctacaac cggcctgcca
    19261 tatcagtcaa cttgatgcca gaacacagca acatgagatg ttaaaaacca cgggtcgccc
    19321 cgtgactcgt gggctcacct taggcctgaa ggatattctc aatgcccgcg aggttttgtt
    19381 attggtgact ggcgaaggaa agcaggatgc gacagaacgt tttctcacgg ctaaagtctc
    19441 caccgctatc ccggcttcgt ttttatggct gcacagtaac tttatttgtt taattaatac
    19501 ctaaccagat gaaaatttgc tcaaaatttt taaattaact tattcaacag tcacgcatta
    19561 tattaaataa catttgactg ggttgaacat aacgccaata gcaaaaggag ttatgttcaa
    19621 caatttaatt ttagatatac atataacttg ttctattaat aaatcacctc gtattctgac
    19681 tcggcccctg gcgcagcaaa gtcagg
//
LOCUS       DAZGTR010000082        19417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGTR010000082 DAZGTR010000000
VERSION     DAZGTR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.082"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..863
                     /locus_tag="ACHLCY_003241"
     CDS             <1..863
                     /locus_tag="ACHLCY_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0370442.1"
                     /translation="LVIFECIYQHLSISKAAESLYITPSAVSQSLQRLRAQFNDPLFI
                     RSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFIIYGPQLISCSNNSML
                     IRCLRQDASVEIECHDILMSAENVEELLVHRKADLVITQMPVISRSVICMPLHTIRNT
                     LICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEKFMNRKISFRGSSLL
                     TIINSIAVTDLLGIVPYELYNSYRDFLNFKEIKLEHPLPSIKLYISYNKSSLNNLVFS
                     RFIDRLNESF"
     gene            1010..2230
                     /locus_tag="ACHLCY_003242"
     CDS             1010..2230
                     /locus_tag="ACHLCY_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0370443.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2203..2831
                     /locus_tag="ACHLCY_003243"
                     /pseudo
     CDS             2203..2831
                     /locus_tag="ACHLCY_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
     gene            complement(2832..3992)
                     /gene="ybdL"
                     /locus_tag="ACHLCY_003244"
     CDS             complement(2832..3992)
                     /gene="ybdL"
                     /locus_tag="ACHLCY_003244"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP0370444.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4101..5189
                     /gene="hcxA"
                     /locus_tag="ACHLCY_003245"
     CDS             4101..5189
                     /gene="hcxA"
                     /locus_tag="ACHLCY_003245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP0370445.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5199..5396)
                     /locus_tag="ACHLCY_003246"
     CDS             complement(5199..5396)
                     /locus_tag="ACHLCY_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0370446.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5578..7683)
                     /gene="cstA"
                     /locus_tag="ACHLCY_003247"
     CDS             complement(5578..7683)
                     /gene="cstA"
                     /locus_tag="ACHLCY_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP0370447.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQTCFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7864..8277)
                     /gene="entH"
                     /locus_tag="ACHLCY_003248"
     CDS             complement(7864..8277)
                     /gene="entH"
                     /locus_tag="ACHLCY_003248"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP0370448.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGMELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8280..9026)
                     /gene="entA"
                     /locus_tag="ACHLCY_003249"
     CDS             complement(8280..9026)
                     /gene="entA"
                     /locus_tag="ACHLCY_003249"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP0370449.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9026..9883)
                     /gene="entB"
                     /locus_tag="ACHLCY_003250"
     CDS             complement(9026..9883)
                     /gene="entB"
                     /locus_tag="ACHLCY_003250"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP0370450.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9897..11507)
                     /gene="entE"
                     /locus_tag="ACHLCY_003251"
     CDS             complement(9897..11507)
                     /gene="entE"
                     /locus_tag="ACHLCY_003251"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP0370451.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11517..12692)
                     /gene="entC"
                     /locus_tag="ACHLCY_003252"
     CDS             complement(11517..12692)
                     /gene="entC"
                     /locus_tag="ACHLCY_003252"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP0370452.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13061..14017
                     /gene="fepB"
                     /locus_tag="ACHLCY_003253"
     CDS             13061..14017
                     /gene="fepB"
                     /locus_tag="ACHLCY_003253"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0370453.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSTEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(14021..15271)
                     /gene="entS"
                     /locus_tag="ACHLCY_003254"
     CDS             complement(14021..15271)
                     /gene="entS"
                     /locus_tag="ACHLCY_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP0370454.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15382..16386
                     /gene="fepD"
                     /locus_tag="ACHLCY_003255"
     CDS             15382..16386
                     /gene="fepD"
                     /locus_tag="ACHLCY_003255"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP0370455.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16383..17375
                     /gene="fepG"
                     /locus_tag="ACHLCY_003256"
     CDS             16383..17375
                     /gene="fepG"
                     /locus_tag="ACHLCY_003256"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP0370456.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSITMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17372..18187
                     /gene="fepC"
                     /locus_tag="ACHLCY_003257"
     CDS             17372..18187
                     /gene="fepC"
                     /locus_tag="ACHLCY_003257"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0370457.1"
                     /translation="MNESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18184..19317)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLCY_003258"
     CDS             complement(18184..19317)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLCY_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP0370458.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4638 a         5435 c         4935 g         4409 t
ORIGIN      
        1 ttcttgtcat atttgagtgt atttaccagc atttaagcat tagcaaagct gctgaatcac
       61 tatatatcac cccatcagcc gtgagccagt cgttacaacg cttacgtgcg caatttaatg
      121 accctttatt cattcgctca ggaaaaggta tcgcgccgac gacaacaggg ttgaatctgc
      181 atcaccatct tgagaaaaac ctcaaaggcc ttgaacaaac aattaatata gtcaataagt
      241 ccgaactaaa gaaaaacttt attatttacg gccctcagct catttcttgc tctaataata
      301 gtatgctgat ccgctgccta cggcaagatg cctctgtcga gattgagtgc catgatatcc
      361 tcatgtcagc agagaatgtt gaagaactgt tggttcaccg caaagccgat ctggtgatca
      421 cccaaatgcc agtgatcagc cgttccgtca tttgcatgcc attgcataca attcgcaata
      481 ctctaatttg tagtaataag catccgcgaa ttactgacaa tagcacttat gaacaaatta
      541 tggcagaaga gttcacccaa ttaatatcca aatcagcagg agttgatgat attcaaatgg
      601 aaatagacga gaagtttatg aatcgcaaaa tttcatttcg tggttcctct ttgctgacaa
      661 tcattaatag cattgcagtt acagatttac tgggaatagt cccctatgag ctttataatt
      721 cctaccgtga ctttcttaat tttaaagaga taaaactaga acatccgtta ccttcaataa
      781 agctttacat ttcctataat aaatcttctt taaataattt ggtgttctcc cgatttattg
      841 accgtctaaa tgagagtttt tgatatataa ctacagacag ctactataac tttcatctat
      901 ttattcacag cgcgaatgaa aaataaaaca ctctgctttg cagtgtttac tttataatta
      961 acagacttat ctcactgatc accctgtaac gttcagagag cgtcttcaga tgtctattta
     1021 taaaattcct cttccgctca atattctgga agctgcacga gagcgtatta cctggacact
     1081 caacacccta ccccgcgttt gtgtctcatt ttctggcggc aaagactctg gtttaatgct
     1141 gcatctgaca gccgaacttg cccgacaaat gggcaaaaaa atctgcgttt tgtttatcga
     1201 ctgggaggcg caattctctt gcaccattaa ctatgttcag tccctgcgcg agttgtacac
     1261 cgatgtcatc gaagagtttt actgggttgc gctcccgctt acgacgcaaa attccctttc
     1321 acaataccaa cccgaatggc agtgctggga acctgatgtc gaatgggtgc gtcagccccc
     1381 gcaagatgcg ataaccgatc ctgacttttt ctgcttttac cagccaggca tgaccttcga
     1441 acaatttgta cgtgagtttg ccgaatggtt ttcacaaaaa cgtccggcgg cgatgatgat
     1501 cggcatccgt gcggatgagt cctacaaccg ttttgtcgcc atcgccagtt taaataaaca
     1561 acgttttgcc gacgataaac catggactac ggccgcacca ggcggtcata gctggtacat
     1621 ttaccccatt tacgactgga aagtggctga tatctggacc tggtatgcaa atcatcaaaa
     1681 tctctgcaac ccactgtata acctaatgta tcaggcaggc gttcctctgc gtcatatgcg
     1741 aatttgcgaa ccttttggcc cggagcaacg acaaggatta tggctctatc acgttatcga
     1801 accggatcgc tgggctgcta tgtgcgcacg agtcagcggg gtaaaaagtg gcggcattta
     1861 cgccggacat gataatcatt tctatgggca tcggaaaatc ctcaagccag aacatttaga
     1921 ctggcaagaa tatgcattat tgctgctcaa tagcatgccg gaaaaaacag ctgagcatta
     1981 ccgcaataaa atcgccattt atttgcactg gtatcagaaa aaaggcatcg aggttccaca
     2041 aacccagcaa ggggacattg gcgcgaaaga tatcccctcc tggcggcgga tatgcaaagt
     2101 tttactcaat aacgattact ggtgtcgggc attatcattt agtcctacga aagcgaagaa
     2161 ctatcagcgt tataacgaac ggataaaagg aaaacgtcag gaatggggga tactatgcaa
     2221 caacgattaa cgcaggattt aacgcaattt ctcgccagct tgccggaaga tgatcgcatc
     2281 aaagcgatta atgaaatccg catggcgatc catcaggtta gcccctttcg tgaagagcca
     2341 gtggattgcg ttctgtgggt taaaaacagc caacttatgc ctaatgatta caacccaaac
     2401 aacgtggcac cacccgaaaa aaactgctga aaaaatccat tgaaatagat ggttttaccc
     2461 aacccattgt ggtcacgcat acggataaaa atgccctgga aatcgtggat ggttttcacc
     2521 gccatgaaat tggtaaaggc tcatcatcat taaaattacg cttaaaaggt tatttgccgg
     2581 tgacctgtct ggaaggtacg cgtaatcaac gcatcgccgc gactattcgt cataatcgcg
     2641 ctcgtggacg ccatcaaatc accgccatgt cggaaatcgt ccgtgaactt agccagttgg
     2701 gatgggacga taataaaatt ggcaaagagc taggaatgga cagcgacgaa gtgctgcgcc
     2761 tgaagcaaat taacggcctg caagagctgt ttgccgaccg tcaatattcc cgcgcctgga
     2821 cggttaaata actaaagctg gcgcaggcgt tcagctgctg ccagcaacgt cgattccttc
     2881 ttggcaaaac agagacgaat cagtttatgt gggaagggat cggcgcaaaa caccgacagc
     2941 ggaatcgccg ctacgccgtg ctcctgcgtc agccactggc aaaactcaac atcatccagg
     3001 gtagaaacct cgctgtaatc caccagcaaa aagtatgtac cttcacacgg taaaatctcc
     3061 agccggcttt catttaaagc attcaccaga atatcgcgct tctggcgata aaagtccggt
     3121 aacgcaagat aatgctcagg ttctgcacgt agcatatcag caagcgccag ctgtgccggg
     3181 gtattcaccg aaaaggtcag atactgatgt accttgcgaa tttcggcgct gatgggcgct
     3241 ggcgcaacac aataacccac tttccagccg gtcatatgat aggtcttgcc aaatgaagaa
     3301 accgccactg cccgctcacg cagctgcgga tgcgccagca cactggcatg gccctgttgt
     3361 gaaaagttga tgtgctcgta gacttcatcg ctaatgacaa aaatctcgtg cccggcgatc
     3421 gcctgccaca aagcggcgaa atcagcctgc tgccagacag ttgcactggg gttatgcgga
     3481 gtgttgagga tcaccagtct ggtgcgctcg cttaataatg cggcaaattc ctgccagtca
     3541 acgcgaaaat gcggtggttg cagtgccata cgcttcacta ttcccccaga aagcgcgatg
     3601 gcgggggagt aactgtcata gctgggatca aaacaaatca cttcatcgcc attgcgcacc
     3661 agtgcggtaa tcgccgcgta taacgcttcc gtcgcccctg ccgttacggt gatatcgcta
     3721 tcggcatctg gttgatagcc atacaaacgt tccgttttct gagcaatcgc ctcgcgcaag
     3781 gcctgcacgc cggtcatggg cgcgtattgg tttgccccct gggcaacgtg gtacgccagc
     3841 cgctcctgta aatagcgcgg accatcaaaa tcaggaaagc cttgcgacag gttaatcgcc
     3901 tggtgttgct gcgccagcgc gctcatctgg gtgaaaatag tggtgccaag ttgtggaagt
     3961 ttgctttgtg gaatcagagg gttatttgtc atttttattg gacctgacga agttgcgtgt
     4021 tgaaggcact ataacacgat gctagtattt ggcaatcaag acgtttagat gtctaaataa
     4081 aacaataagg acaacacaac atgcctcaca atcctatccg cgtggtcgtc ggcccggcta
     4141 actacttttc acatccagga agtttcaatc acctgcacga ttttttcact gatgaacaac
     4201 tttctcgcgc ggtgtggatc tacggcaaac gcgccattgc tgcggcgcaa accaaacttc
     4261 cgccagcgtt tggacttcca ggggcaaagc atattttgtt tcgtggtcat tgcagcgaaa
     4321 gcgatgtaca gcaactggcg gctgagtccg gtgacgatcg cagcgtggtg attggcgtcg
     4381 gcggcggcgc actgctcgac accgcgaaag ccctcgcccg ccgtctcggt ctgccgtttg
     4441 ttgccgttcc gacgatcgcc gccacctgcg ccgcctggac accgctctcc gtctggtata
     4501 acgatgccgg acaggcgctg cattatgaga ttttcgacga cgccaatttt atggtgctgg
     4561 tggaaccgga gattatcctc aatgcaccgc aacaatatct gctggcgggg atcggtgaca
     4621 cgctggcgaa atggtatgaa gcggtggtgc tggctccgca accagaaacg ttgccgctaa
     4681 ccgtgcgact ggggatcaat aatgcgcagg ccattcgcga cgtattgtta aacagtagcg
     4741 aacaggcgct gagcgatcag caaaatcaac agttaacgca atcattttgc gatgtggtgg
     4801 atgctattat tgctggtggt gggatggtcg gtggtctggg cgatcgtttt acgcgtgtgg
     4861 cggcggctca tgccgtgcat aacggtctga ctgtcctgcc gcaaaccgag aaatttctcc
     4921 acggcaccaa agtcgcttat ggcattctgg tacaaagcgc cttgctgggt caggatgatg
     4981 tgctggcgca attaaccggg gcgtatcagc gttttcatct gccgaccacg ctggcggagc
     5041 tggaagtgga tatcaataat catgcggaga tcgacaaagt gattacccac accctgcgtc
     5101 cggtggaatc cattcattac ctgccagtca cgctgacacc agatacgttg cgtgcagcgt
     5161 tcgaaaaagt ggaatcgttt aaagcctgac gtgcaaattt agcagcagcg cgcgccgcct
     5221 tttccaccgt agcgcgcgtc ctgccgctca cggaaaaact cttcgtaggt catcggcgtt
     5281 tgatcgggat ggttaacccg catatgttcg acatagttgt cgtaatcagg cataccaatc
     5341 atcagcttcg ccgcctgacc taaatatttt ccggcttttg ccagtgaatc aaacatcttg
     5401 gcctccgcta accatgttgc tgaaagagtg ccggatgcgg cgtgaacgcc ttatccggcc
     5461 tacaaatgct atcaacgcgc tcggtcagtc ccctcgcccc atcggggaga gggttagggt
     5521 gaggaggaac cctcggcact atgccaacat tcgccaacat cccccctcac tctgacttta
     5581 gtgtgcgcct tttgcctgcg ccacgatctc ctcgacattt tccggcattg gctcatacgg
     5641 cgtttctttc gccgttggct tcggatcttt caatgccgcc agtgccgtct taatcgagaa
     5701 cagcgccaga accaccacga ccaccataaa gaagatggtt aacccggcat ccagacggtt
     5761 gttgaacacc agttgcgcca gctgtgactc agtatactgc gacggaatat tgccgctgtc
     5821 gatcattgcc tggaacttat tagcaatggc caggaagccg actttcgcat ccgggctaaa
     5881 cgctttctgc cagcctgcgg tcagggtaca aatcagcagc caggccgttg gtaccagcgc
     5941 cacccaggcg taacgttgac gtttcatctt gaacaacacc acggcacaga gcatcagcgc
     6001 catccctgcc agcatctggt tggcaatacc aaacagcggc cacagagtgt taatgccgcc
     6061 cagcggatcg accacgccct gatggaggaa gtagccccac gccagtacgc acagcgctgt
     6121 tgccagcagg ttagcaggca gtgaatcggt ccgcttcagg cccggagaaa ccacgcccag
     6181 cagatcctgc aacataaagc gcgcagcacg cgtacccgca tctaccgccg tcagaataaa
     6241 cagagcttca aacagaatgg cgaagtgata ccagaacgcc acatccatca taccacccag
     6301 cgcaccgtgc aggatgtaag ccatccccac cgccagcgtc ggcgcaccgc ccgcacggga
     6361 aatgatcgac tgttcaccca cttcgctggc aatctggttg agcgtatctg gtgtaatgct
     6421 aaagccccag ctactcacca cctgcgcggc agaagcgact acatccgccg tccctgccgg
     6481 agccagcacc gccattgggc tgttcatggc aaaatacacg cccggatcga tgatacaggc
     6541 agaaaccagc gccataatcg ccacgaagga ttccatcaac atcccaccgt agccgataaa
     6601 gcacgtctgc ccttcgttcg ccagcatctt cggcgtggtg ccggaagaga tcagcgcatg
     6661 gaagccagac accgcaccac aggcgatggt gataaacagg aacgggaaca ggttaccggt
     6721 ccataccggg ccagtgccat cgacaaattt ggtcagcgca ggcatggtca gcgtcgggcg
     6781 cataatcaaa atgcctaccg ccagaccgac gatagtcccg attttcagga aggtcgagag
     6841 atagtcacgc ggggccagca gcaaccacac cggcagcacc gccgccacaa aaccgtagcc
     6901 caccagcatc caggtcagct gcacgccggt aaagtcaaag tacggtgccc aggtcggact
     6961 ttctgccacc cagccgccag agataatggc gaaaatcagg aacaccaggc caatcactga
     7021 cacttcgcca atgcggcccg gacgcagata gcgcaggtag attcccataa aaatcgccag
     7081 cggaatggtg aacgcaacgg tatacgttcc ccacgggcta tgagtcaggg ctttcaccac
     7141 gatcatcgcc agtactgcaa ggataatgac catgatcata aagcaggcta ccagcgcaat
     7201 caccccggca gttggcccca tctcttcttt gaccagctca cccagcgatc gaccgtcacg
     7261 gcgcgtagaa acaaacagca ccatgaaatc ctgcaccgca ccagcgagaa ccaccccggc
     7321 gagcagccag atcatccctg gcaggtaacc catttgcgcc gccagtaccg gcccaaccaa
     7381 cggacctgct ccggcaatgg ccgcaaaatg gtgaccgaac agcactttct tgtccgtcgg
     7441 cacatagtcc agcccgtcgt tatggcgcac cgctggcgtc atacgcgtcg ggtcaaccgc
     7501 cagcacattt ttggcgatat acagcccata aaaacggtaa gcgatcagat agatacagac
     7561 cgacgccacc acaatccaca gcgcgttgat ctgttcccca cgatttaaag caatgtatcc
     7621 cagagcaaat gctcccatta cagagagcac tgtccagacg aggtatttcc ctgatttgtt
     7681 catagttgtt atccgtgtgc gtgtccatag agatgttaca ttttgtttct ataacatctt
     7741 tgtaagattt aacaacacgg taaccatgta aatcggagtt gaaaccaata tttaacttaa
     7801 caatgttaac tcgatcactc cgttaaccga gttttcctgc aatctcattt aattctgtcg
     7861 ggctcatccc aaaactgccg tacccagccg acaagtgcag caacgccgcc cctgttcatc
     7921 gaaaacgacg atttcccagc tctgattttg ccgcccaaga tgcagcggct ggcagacgcc
     7981 gcgtaccttg ccttcagaca ccgggcgatg atgggtggcg ttaagttcca tacccaccac
     8041 acactgcccg tcacgggtca tcataaatcc ggccatcgat cccagcgttt cagccagcgc
     8101 cgccgacgcg ccgccatgca gcaggccaaa cggctgatga gtacgggtat caaccggcat
     8161 ttcggcttcc agcacatcat ctcccagacg ggtatacaca attcccagat gcgccaccat
     8221 tgtgttatcg ctggtggcgt tcagttcgtc gagcgttaaa tggcgtttcc agatcatgct
     8281 tatgccccca gcgttgagcc gccatcgacc acaatatcct gtagggtgat atggctggcg
     8341 aggtcagagg cgaggaacaa aatcgtgttg gcgatctctt gtggacgggc gattttcccc
     8401 agcggaatgc cgagtttaaa ctgctcgcca aagccgcgaa tacgctgttc ttcggcgtca
     8461 tcgctcaccc acagcgtgcg ttgcatatcg gtgtcggtgg agccaggcga aaccacatta
     8521 cagcgcacgc cgctacccgc cagttccagc ccgacgctca acgccaggct tttcagcgcc
     8581 gctttcgatg cgccataagc actcatacca atacgcggcg tgtgtgcggc gtcggacgcc
     8641 acagtgacaa tcgccccgcc ccgctgacgg cgaaactggt tcatggtttg ctggaacagg
     8701 ttaaacgcac cgccgacgtt aaccgcaaaa gtctgctgcc agtcctcttt actgagctga
     8761 tcggtcgcgc ccatgcgcaa aattcccgcc gcattgatca gcacgtccag tcgctccgtt
     8821 tcagctaaca gtcgctgaca tacctgcgcg acctgcgcag catcggcaac atccatcact
     8881 tcggtcgcaa agggatattg ctcctgagcg aacgcttgat caaaaccggt aactttcgct
     8941 cccgcctcaa caaacgccag cgccgtggcg tagccgatac ctttacctgc gccggttacc
     9001 cagacatttt taccgctgaa atccattatt tcacctcgcg ggagagtagc ttccaccagg
     9061 cgtcgatggt cgggtttttc gccagcatga caaagtcgat atcaccatgc actttgcgcc
     9121 agcgcgccgc cagcgccatc atgcgcaccg aatccagacc gtagtcgatc aggttgtcgt
     9181 catcgaacgg ttcatcggac tcgtccagca acggcaggat aacctcacgc agcgccgctt
     9241 tgctggcggg gacaggtgct ggcagtaatt cttcagtcat caccacccgg ccagaccgcc
     9301 cggccacata tttcagcgac atcaaatgct cgtcacggct gaaatcggcc agcgcatccg
     9361 ccaccataaa cggtttaata tcgcgcataa atgcgtcggt tgcggtggtc atacagccaa
     9421 tgtgggcata taccccggta ataatcagct ggttacgtcc actctctttg agcatttgct
     9481 ccagcggaga acgatgaaac gcgctgtagc gccacttcac cagcaccgtg tcgtcggcat
     9541 ctggcgtcag gcgatccacc accttttgct gttccggcga acgggtcagg cccggccccc
     9601 acatatcatt caacagcgcc cgatcttcat cgctctgctc tttcggctgg gcggtgtaat
     9661 aaaccggaat attgtgctgt ttgcagtagt cgcgcagcgc agcaatattc gcgatcacct
     9721 gctccatcat cgggcagttc tcgccccaga agctgacaaa atagtcctgc atatcatgga
     9781 ttaacaacgc ggcacgttgc ggttcaaagg cccagtcaac tttattctta gggatatcgt
     9841 gagactccgg cagtgcgtaa gcctgtaatt ttggaatagc catcgtgttc tctccttcag
     9901 gctgatgcgc gtgacgccag ccactgacgt aattgttttt tatcgacttt cccgaccgcc
     9961 gtcagcggaa gtgaatctac acactccacg cgatccggta atttaaattc ggcaataccc
    10021 tgttcacgca ggaaacgacg cacctgcacc gcgcgcagcg gctcttttac caccagataa
    10081 gcgcagcttt tttcgcccat cagctcatct tccatgctca ccagtgcggc gtagatcacc
    10141 gccgggtggc gcagcagcag gttttcgatc tcttcggcag cgatcttctc accgccacgg
    10201 ttgatctgat ctttctcgcg cccctgcacg gtgatgtacc cctctggatc aatagagatc
    10261 agatcgccgg aacagtaaaa accgttggca tcaaaggcgc tggcattgtg ctgcgggctt
    10321 ttgtaatagc cacggaaggt gtacggaccg cgggtcatca ggcgtccaac ttccccttgc
    10381 ggcagtggat ttccttcggc atcggcaacc cacacttcat cgtccggaca cattgggtaa
    10441 ccctgggtat ggataatttt ctccgcgcta tcatcaagac gggtgtagtt caccagccct
    10501 tccgccatgc caaacacctg ctgcaactgg cagccaatct cagcgggaat acgcgccgca
    10561 agggtggcag aaagacgtgc gccgccgacc tgtaacagtt tcagcgaggc aagctgcgcc
    10621 cggctttcac cttcggtcag cgcctgcaac cacaggctga ctgccggtgg caccagcgcg
    10681 gtgacgttca cctgatgttt ttcaatcaat gggaagcaaa gcgtggcgct gggatcggcc
    10741 gccagaacaa ccgttcctcc ggcaagaaag acgcccagcg atcccggcga actcatggcg
    10801 tagttatgag ccgccgggat cgcgcacagg tagcgtgtct gttgtgtgaa ctgacaaatc
    10861 tcgacgctac gacgcacgct gtagtagtag tcgttatgag tgcgcgggat cagtttcggt
    10921 gtgccggtgg tgccgccgga aagctggaaa taggccactt catcagcagg tgatggcgtg
    10981 gcagtaaaat cctcagccgg atggttaatc gcatcctgca agttatgctc accgctgtcg
    11041 ttgtgcagtt gcaccacgcg aatggaggaa tgttctgtga cgaaagtatt gaggaaatca
    11101 tccccgctaa acagcgcatg ttggcgatcg gcaatcagca atgcgggttc aatctggctg
    11161 gcataggcgt tcagttcact acgctgatgg ctgaacagcg ccagcaccgg cgcaacgccc
    11221 agtttcagca gcgcgaaaaa ggtaatatac aattcagcga cgttacccag ttgtaccagc
    11281 gcggtttcac caggtttaat gccctgacgg cgtaaactac acgcgaggtt atccgccgcc
    11341 tgattcagct cccggtaact caactgtcgc tcgccgtcga taaccgcgat gctgtcactc
    11401 gcagcgtggc gagtcagaat gtcggtcagc ggcaaatcct gccagtagcc tttttcccga
    11461 tagcgacggg caaactcttc cggccagcgg gtgaatggaa tgctcatcct cgctccttaa
    11521 tgcaatccaa aaacgttcaa catggtagaa agtttgacgc ctgtttcgcg ccactcaccc
    11581 aacggtgacg acgcaggcac aatccccgct ccggcaaaca gacgcacctg attttcccgc
    11641 agcttcgcgc agcggatggt caccacccat tcgccgttac cttcgctgtc acaccaaccc
    11701 acaatgccgc caaacagttc gcggtcgaat ggctccagtt cagcaataac ctgggtcgcg
    11761 gcctgatgcg ggaaaccgct cagcgcgggg gttggatgca gcagacaggc cagagtcagt
    11821 gcgttttctt gcgaattcgc tttaccttca aagggagttg cgagatgcca cagcgtcggc
    11881 gtggtaatca attgtggaga ggagggaacg tgtaactcac tactgcgttc gcgcagtacc
    11941 tctttcatcg cctgagtcac cagttcatgt tcatggcgat ctttttctga cgccagcaga
    12001 cgattacccg cttcgcgatc gagcacttca tccggctgac gacgcgcgga accggctaac
    12061 ggaatggagc taaaacgctc gccgtcttta cgtagcagca gttccgggct ggcccccagc
    12121 aggacgccac catcagccag cgggacatgg aagttgtaac taaccgggtt ttgcgcaatc
    12181 aaccgttcca gcaatacgcc actatcaatg gcggcgtcag tggtgatatc aatcaatcgt
    12241 gacaacacca ctttgtcgac ctgcggcgtg gcggtaagtg cggcggcgcg ggcaaccatc
    12301 tgttcaaacg tggtttgttc aggaattgcc tggcgttcca ccacgttcag cgactggctg
    12361 cgggtaaaac ggcgggctga ggtctgtttt tcctggcggg agaacgactg ccaggattcg
    12421 ggaatataca gcgacgaagg ctgacgtgga tcgaagggaa tcgccccgac catcaccgga
    12481 tttttgatgc cctgcgcttt ggcatcggca aacagcgcgg cgagtttttg ctggaaggga
    12541 ctgtcgggcg aatccccgtt cacagccggt tcatcgaagc gggcgaaaca tcctgacgtc
    12601 gtaaaactgc ggtacggcga cataaagaaa aagcgattgg gcgcaagtgt tgccatggtc
    12661 tgctgtactt cctcagccag tgacgtatcc atatcatcct ccacaaaatg ataaaggctt
    12721 taataatgat tatcatttat attttcggtc gctaacctaa aagcaaacgc gcactatgtc
    12781 aactcttgag gtaacgcaat ctgcaactcg gggttgcatc tggttgtggt tacaatgaaa
    12841 atgagaagca ttattgatgg attcgcataa gcgcaatgtg atgtcctgcg ccgttctgcc
    12901 ccctctccct tccagggtga gggctggggt gagggttaat gttcgcacca gtgctggctg
    12961 ttcccctcac cctctcccca aaggggcgag gggacggatt gtgcgctttg tcgaatttgt
    13021 cattacgccc ataaccttat taataacagg aagctgattt gtgagactcg ccccgctcta
    13081 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgcag ttcaggccgc
    13141 tgactggccg cgtcagatta ctgacagccg tggcacacat acactggaaa gccagccgca
    13201 gcgtattgtt tccaccagcg tcaccctgac cggctcactg ctggcgattg atgctccggt
    13261 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgcg gatgaccagg gctttttacg
    13321 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    13381 caccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    13441 ttcggcgctg gcactgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    13501 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    13561 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    13621 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    13681 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    13741 gaagttgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    13801 gcttggtggg gaaaatctgg ctgctgggtt aaatggcgag tcactattcc tgttcgccgg
    13861 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc ttcccgcagt
    13921 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgtctggatt actacagcgc
    13981 cacgcaagtg ctggaacgac ttaaggcgct gttttaagca ttaactgtca gatgctgtca
    14041 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    14101 cgataatcaa caaaccaaaa ccgcttgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    14161 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    14221 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    14281 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    14341 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    14401 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    14461 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    14521 cagccagcgc cggatacagt acccgcaccg cgctcgccat cgtcaataaa ccacccagca
    14581 gcgcaatccc gcccaccagc gggctggcaa gcagaaaacg aaatcctgcc agtaatgatt
    14641 tcaacggatg ctcacgcggc tgcggtggcg gtggcaacgc cggaaggctt aacaacggta
    14701 gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg ccaccggtcg
    14761 ccagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg gtcaacatgg
    14821 tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgctggtgtc gccgccaata
    14881 gcgccgtaac gccaagcgat gcgaaaaaac catcccataa accaagtaaa tagattgcca
    14941 gcaatgacgg ctccggcagc agtgcattaa ggcacagtcc aatgaagcca atgccacagg
    15001 tgccgcgcgc cagcaaaatc acttttttgc gctcatagcg atccgccagc acaccgccaa
    15061 ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc acctgccagg
    15121 tagaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaca
    15181 caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc
    15241 tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa
    15301 tacctattct taggctgcac atgctaacat atccaaataa gatcgataac gataattaat
    15361 ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg
    15421 cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat tggggcaaaa
    15481 tcactccccg cttccgtagt gctggaggcc ctctccggca cctgccagag cgccgactgc
    15541 accatcgtgc tcgacgcgcg actgccgcgc acccttgccg gtttacttgc aggcggcgcg
    15601 cttggccttg ccggggcgtt gatgcaaacc ctcacccgaa atccacttgc cgaccccggc
    15661 ttgcttggcg tgaatgccgg agccagcttt gccattgtgc tgggtgcggc gctgtttggt
    15721 tactcatccg cgcaggaaca actggcgatg gccttcgccg gggcgctggt ggcctcattg
    15781 attgttgcct ttaccggcag tcagggcggc gggcagttaa gtccggtgcg tttaaccctg
    15841 gcgggcgtgg cgctggcggc ggtgctggaa gggctgacca gcggcatcgc cctgcttaat
    15901 cctgatgtct acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta
    15961 cataccttaa aagtggtgct gatcccggtg ctgatcgccg gagcaaccgc gctattactg
    16021 agccgcgcgc tgaacagttt aagcctcggc agcgacaccg cgacggcgct gggcagtcgc
    16081 gtggcgcgca cacagttaat tggtctgctg gcgattaccg tgctttgtgg tagtgcgacg
    16141 gcggtagttg gcccgattgc ctttatcggc ctgatgatgc cgcacatggc gcgctggctg
    16201 gtaggtgccg atcatcgctg gtcgctgccc gtcacgctac ttgctacccc tgccctgctg
    16261 ctgtttgccg atatcatcgg gcgggtgatt gtgcccggcg aactgcgcgt ttctgtggtc
    16321 agtgcgttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggc
    16381 gcatgattta cgtctctcgc cgattaatca tcacctgttt gctgttggtt tccgcctgtg
    16441 tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaacc tcgcaggtat
    16501 tcgccgcgct gatgggcgat gcgccgcgca gtataacgat ggtggtcacc gaatggcgtt
    16561 taccacgcgt gctgatggcg ctgttgattg gcgcggcact gggcgtcagc ggcgcgattt
    16621 ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt aacaccgggg
    16681 cgtggagcgg cgtgctggta gcgatggtgc tgtttggtca agacctgacg gctatcgcgc
    16741 tggcagcaat ggtgggcggc attatcactt cgctgttggt ctggctgctc gcctggcgta
    16801 acggcatcga cacctttcgg ttgattatta tcggtatcgg cgttcgtgcc atgctggtgg
    16861 cctttaatac ctggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt
    16921 ggaatgcagg atcgctcaac ggcctgacgt gggcaaaaac ctcgccttcc gcacccatca
    16981 ttatattgat gctcattgcc gccgccttac tggtacgccg gatgcgcttg ctggaaatgg
    17041 gcgatgatac cgcgtgtgcg ctgggcgtca gcgtcgaacg ttcgcgtctg ttaatgatgc
    17101 tggttgcagt ggtgcttacc gctgcggcaa ccgcgcttgc cgggccgatt tcctttattg
    17161 ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg gggctgaccc
    17221 aggcggcgct gtgcggggcg ctgttactgc tggcggccga tctctgcgcc caacaactgt
    17281 ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta
    17341 tcgtcttgtt aattcaggag tctcgcaaaa aatgaacgaa tctgtagccc gtttgcgcgg
    17401 cgaacagtta accctcggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat
    17461 acctgatggt cacttcacgg caattatcgg gccaaatggc tgcggtaaat ccactttact
    17521 gcgtacttta agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca
    17581 cattcaacat tacgccagta aagaggttgc acgccggatt ggtctgttgg cgcaaaatgc
    17641 taccacgcca ggcgatatca ccgtgcagga gctggtggcg cgtgggcgtt acccgcatca
    17701 accgctattt acccgctggc gcaaagaaga tgaagaagcg gtaacgaaag cgatgcaggc
    17761 cacgggaata actcatctgg cagatcaaag cgtggatacc ctttctggcg gacaacgcca
    17821 gcgcgcgtgg atcgcgatgg tgctggccca ggaaacggca attatgctgc tcgacgaacc
    17881 cacgacctgg ctggatatca gtcaccagat tgatctactg gaactgttaa gcgaactgaa
    17941 ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta
    18001 cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag cgccgaagga
    18061 gattgtcact gctgaactga ttgagcgcat ttatggtctg cgatgcatga tcattgacga
    18121 tccggtagcc ggaacgccgc ttgtggtgcc gctcggacga acggcacctt caaccgcaaa
    18181 tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca tcgcatagcg
    18241 caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga tcacaataat
    18301 cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat atttaaacgg
    18361 cgtaaaattc acatcgttga tatttgcttt tgttaactgc tcaacaagat actgacgatt
    18421 acgtaattca ccgttcagtt ccgcaacgtc agtgaccgct ttttcaattt ccagtttgcg
    18481 ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc
    18541 attactgtat acgggctttt taattcccgc cgcgttggca atgtcgagtg aataattgag
    18601 gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc
    18661 tttttcaaac tgggttttga tttccagttt attacggacg ttttctagtg actctttcac
    18721 caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct cttcactggt
    18781 tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact
    18841 ggcattgtca tcaaccgctt tcattttttc gctcaatgca acaatagcgc gatgcaaatc
    18901 cagttcatcg attttcgcct cttttaattg gtccatcaca taaggtgatg aacgcaggta
    18961 ctcttccagc aagctaaccg actgaaactt cttgataaac aggttaaatg cttctgtacg
    19021 atcaattttg atatccagat ccagcactcg aagcttagtg aatgttttct ccagctcttg
    19081 ccactgaaca ggttctggag gcgtgacaac cgccgcgctg gtccattttt gcggcaggat
    19141 gaaagagatc agcaagcctg cgcaggcaaa cgcaaaaatg accgccatga ccgttttttt
    19201 ggcccgccat aaaactgcga ttagattaag taaatcaatt tcattattac tgggcgacgc
    19261 cagaggatac tcaggaaaat gagcgtcact tccctgttta atattcagtg atgacatggg
    19321 gtataacctg aaaatgagtc caatagggga aatctccacg gcgctcactt taccagttgg
    19381 tgataattat ccgaagctga agtttgtaaa ttccttc
//
LOCUS       DAZGTR010000083        19384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGTR010000083 DAZGTR010000000
VERSION     DAZGTR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.083"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(49..696)
                     /gene="ychE"
                     /locus_tag="ACHLCY_003259"
     CDS             complement(49..696)
                     /gene="ychE"
                     /locus_tag="ACHLCY_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP0370459.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(727..780)
                     /gene="ychE"
                     /locus_tag="ACHLCY_003260"
     CDS             complement(727..780)
                     /gene="ychE"
                     /locus_tag="ACHLCY_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP0370460.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1173..3848
                     /gene="adhE"
                     /locus_tag="ACHLCY_003261"
                     /gene_synonym="adhC"
     CDS             1173..3848
                     /gene="adhE"
                     /locus_tag="ACHLCY_003261"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP0370461.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3972..4868
                     /locus_tag="ACHLCY_003262"
                     /pseudo
     CDS             3972..4868
                     /locus_tag="ACHLCY_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5150..5767)
                     /gene="tdk"
                     /locus_tag="ACHLCY_003263"
     CDS             complement(5150..5767)
                     /gene="tdk"
                     /locus_tag="ACHLCY_003263"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP0370462.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6372..6785
                     /gene="hns"
                     /locus_tag="ACHLCY_003264"
     CDS             6372..6785
                     /gene="hns"
                     /locus_tag="ACHLCY_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP0370463.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLASVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(6929..7837)
                     /gene="galU"
                     /locus_tag="ACHLCY_003265"
     CDS             complement(6929..7837)
                     /gene="galU"
                     /locus_tag="ACHLCY_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP0370464.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8039..9052)
                     /gene="rssB"
                     /locus_tag="ACHLCY_003266"
     CDS             complement(8039..9052)
                     /gene="rssB"
                     /locus_tag="ACHLCY_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP0370465.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGAGGRLRLML
                     SAE"
     gene            complement(9144..10049)
                     /gene="rssA"
                     /locus_tag="ACHLCY_003267"
     CDS             complement(9144..10049)
                     /gene="rssA"
                     /locus_tag="ACHLCY_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP0370466.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10162..10620
                     /gene="ychJ"
                     /locus_tag="ACHLCY_003268"
     CDS             10162..10620
                     /gene="ychJ"
                     /locus_tag="ACHLCY_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP0370467.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10670..11512
                     /gene="purU"
                     /locus_tag="ACHLCY_003269"
     CDS             10670..11512
                     /gene="purU"
                     /locus_tag="ACHLCY_003269"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP0370468.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11672..11756
                     /locus_tag="ACHLCY_003270"
     tRNA            11672..11756
                     /locus_tag="ACHLCY_003270"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11706..11708,aa:Tyr,seq:gta)
     gene            11791..11875
                     /locus_tag="ACHLCY_003271"
     tRNA            11791..11875
                     /locus_tag="ACHLCY_003271"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11825..11827,aa:Tyr,seq:gta)
     gene            11923..12054
                     /locus_tag="ACHLCY_003272"
     ncRNA           11923..12054
                     /ncRNA_class="other"
                     /locus_tag="ACHLCY_003272"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12101..12232
                     /locus_tag="ACHLCY_003273"
     ncRNA           12101..12232
                     /ncRNA_class="other"
                     /locus_tag="ACHLCY_003273"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12416..13093)
                     /gene="narI"
                     /locus_tag="ACHLCY_003274"
     CDS             complement(12416..13093)
                     /gene="narI"
                     /locus_tag="ACHLCY_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0370469.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(13093..13803)
                     /gene="narJ"
                     /locus_tag="ACHLCY_003275"
     CDS             complement(13093..13803)
                     /gene="narJ"
                     /locus_tag="ACHLCY_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0370470.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYGVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13800..15338)
                     /gene="narH"
                     /locus_tag="ACHLCY_003276"
     CDS             complement(13800..15338)
                     /gene="narH"
                     /locus_tag="ACHLCY_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0370471.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15335..19078)
                     /gene="narG"
                     /locus_tag="ACHLCY_003277"
     CDS             complement(15335..19078)
                     /gene="narG"
                     /locus_tag="ACHLCY_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP0370472.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4892 a         5095 c         4644 g         4753 t
ORIGIN      
        1 aatgacgaag taaacggagc atggcagctc cgtttcattt gaaaggaatt aattaagcag
       61 gccggggaaa atccccttaa taccagtaac gataaattca atccccaatg ccatcagcaa
      121 tagccccata atacgcgtaa tcacgttgat gccggtctgg cgtaaaaccc gtaccagcca
      181 cggtgccatg cggaacaatc cccagcaaca taaagcgaac aatgcaatag ccacaaagaa
      241 accaaacaga tagctaatgc tgtgataacg cgtaccccag acgatggtag aactgatcgc
      301 ccctggcccc gccatcaacg gcaacgccaa tggcaccaca ccaatgcttt cacgtaccgc
      361 ggtttctgat ttttcttgct tgttctgttt atcctcgcca agcttgccgc tgatcatcga
      421 catcgctatc gtcaccacca ggatgccccc ggcgatacgg aacgaatcaa ttgatatacc
      481 aaaaagttgc agaatcgtgt cgccgagaaa aagcgagatc cacaagataa tggccacaga
      541 caggttggct gtaaggttag ttttgtttcg cgctgccgct gtctgataac tggtcatgct
      601 gataaagacg ggaataatcc ctaccgggtt gaccagcgca aataacccga tgaaaaattt
      661 gaagtaaacg ggaaaatcaa aaaaggtctg aatcacggtt agctccgaag caaaagccgg
      721 ataatgttag ccataaataa ggttgaaaag acgcgctgac aatacgcctt ttgacagcat
      781 ttttcacctc ctaactactt aaaattgcta tcattcgtta ttgttatcta gttgtgcaaa
      841 acatgctaat gtagccacca aatcatacta caatttatta actgttagct ataatggcga
      901 aaagcgatgc tgaaaggtgt cagctttgca aaaatttgat ttggatcacg taatcagtac
      961 ccagaagtga gtaatcttgc ttacgccacc tggaagtgac gcattagaga taataactct
     1021 aatgtttaaa ctcttttagt aaatcacagt gagtgtgagc gcgagtaagc ttttgatttt
     1081 cataggttaa gcaaatcatc accgcactga ctatactctc gtattcgagc agatgattta
     1141 ctaaaaaagt ttaacattat caggagagca ttatggctgt tactaatgtc gctgaactta
     1201 acgcactcgt agagcgtgta aaaaaagccc agcgtgaata tgccagtttc actcaagagc
     1261 aagtagacaa aatcttccgc gccgccgctc tggctgctgc agatgctcga atcccactcg
     1321 cgaaaatggc cgttgccgaa tccggcatgg gtatcgtcga agataaagtg atcaaaaacc
     1381 actttgcttc tgaatatatc tacaacgcct ataaagatga aaaaacctgt ggtgttctgt
     1441 ctgaagacga cacttttggt accatcacta tcgctgaacc aatcggtatt atttgcggta
     1501 tcgttccgac cactaacccg acttcaactg ctatcttcaa atcgctgatc agtctgaaga
     1561 cccgtaacgc cattatcttc tccccgcacc cgcgtgcaaa agatgccacc aacaaagcgg
     1621 ctgatatcgt tctgcaggct gctatcgctg ccggtgctcc gaaagatctg atcggctgga
     1681 tcgatcaacc ttctgttgaa ctgtctaacg cactgatgca ccacccagac atcaacctga
     1741 tcctcgcgac tggtggtccg ggcatggtta aagccgcata cagctccggt aaaccagcta
     1801 tcggtgtagg cgcgggcaac actccagttg ttatcgatga aactgctgat atcaaacgtg
     1861 cagttgcatc tgtactgatg tccaaaacct tcgacaacgg cgtaatctgt gcttctgaac
     1921 agtctgttgt tgttgttgac tctgtttatg acgctgtacg tgaacgtttt gcaacccacg
     1981 gcggctatct gttgcagggt aaagagctga aagctgttca ggatgttatc ctgaaaaacg
     2041 gtgcgctgaa cgcggctatc gttggtcagc cagcctataa aattgctgaa ctggcaggct
     2101 tctctgtacc agaaaacacc aagattctga tcggtgaagt gaccgttgtt gatgaaagcg
     2161 aaccgttcgc acatgaaaaa ctgtccccga ctctggcaat gtaccgcgct aaagatttcg
     2221 aagacgcggt agaaaaagca gagaaactgg ttgctatggg cggtatcggt catacctctt
     2281 gcctgtacac tgaccaggat aaccaaccgg ctcgcgtttc ttacttcggt cagaaaatga
     2341 aaacggctcg tatcctgatt aacaccccag cgtctcaggg tggtatcggt gacctgtata
     2401 acttcaaact cgcaccttcc ctgactctgg gttgtggttc ttggggtggt aactccatct
     2461 ctgaaaacgt tggtccgaaa cacctgatca acaagaaaac cgttgctaag cgagctgaaa
     2521 acatgttgtg gcacaaactt ccgaaatcta tctacttccg ccgtggctcc ctgccaatcg
     2581 cgctggatga agtgattact gatggccaca aacgtgcgct catcgtgact gaccgcttcc
     2641 tgttcaacaa tggttatgct gatcagatca cttccgtact gaaagcagca ggcgttgaaa
     2701 ctgaagtctt cttcgaagta gaagcggacc cgaccctgag catcgttcgt aaaggtgcag
     2761 aactggcaaa ctccttcaaa ccagacgtga ttatcgcgct gggtggtggt tccccgatgg
     2821 acgccgcgaa gatcatgtgg gttatgtacg aacatccgga aactcacttc gaagagctgg
     2881 cgctgcgctt tatggatatc cgtaaacgta tctacaagtt cccgaaaatg ggcgtgaaag
     2941 cgaaaatgat cgctgtcacc accacttctg gtacaggttc tgaagtcact ccgtttgcgg
     3001 ttgtaactga cgacgctact ggtcagaaat atccgctggc agactatgcg ctgactccgg
     3061 atatggcgat tgtcgacgcc aacctggtta tggacatgcc gaagtccctg tgtgctttcg
     3121 gtggtctgga cgcagtaact cacgccatgg aagcttatgt ttctgtactg gcatctgagt
     3181 tctctgatgg tcaggctctg caggcactga aactgctgaa agaatatctg ccagcgtcct
     3241 accacgaagg gtctaaaaat ccggtagcgc gtgaacgtgt tcacagtgca gcgactatcg
     3301 cgggtatcgc gtttgcgaac gccttcctgg gtgtatgtca ctcaatggcg cacaaactgg
     3361 gttcccagtt ccatattccg cacggtctgg caaacgccct gctgatttgt aacgttattc
     3421 gctacaatgc gaacgacaac ccgaccaagc agactgcatt cagccagtat gaccgtccgc
     3481 aggctcgccg tcgttatgct gaaattgccg accacttggg tctgagcgca ccgggcgacc
     3541 gtactgctgc taagatcgag aaactgctgg catggctgga aacgctgaag gctgaactgg
     3601 gtattccgaa atctatccgt gaagctggcg ttcaggaagc agacttcctg gcgaacgtgg
     3661 ataaactgtc tgaagatgca ttcgatgacc agtgcaccgg cgctaacccg cgttacccgc
     3721 tgatctccga gctgaaacag attctgctgg atacctacta cggtcgtgat tatgtagaag
     3781 gtgaaactgc agcgaagaaa gaagctgctc cggctaaagc tgagaaaaaa gcgaaaaaat
     3841 ccgcttaatc agtagcgctg tctggcaaca taaacggccc cttctgggca atgccgatca
     3901 gttaaggatt agttgaccga tccttaaact gaggcactat aacggcttcc acaacaggga
     3961 gccgttttct tatgccactt ctcaatgatc tgctcgattt cagtgaccat ccgcttatgc
     4021 ctccgccctc tgcacaacta tttgcagaac accttcccac cgagtggata caacactgcc
     4081 tgacgctttc tgctcatgcg accgttcgcc gccgtcgttt accgggggac atggttatct
     4141 ggatggtggt gcaatgagcc aattaccgat gttgttcgcc gtctgaacct gagcgcggat
     4201 ggcgaagcgg ggatgaacct gctggcccgc agcgctgtca cccaggcgcg tcagcgcgtg
     4261 ggggccgccc cagtggaatg gctcttccgc cagaccgcac agacaggggc gcggaacgtt
     4321 acctgaagga tgactggcac ggcctgcaac tttttgccat tgatggcgca cagttcagga
     4381 cacctgataa acccgagctg cgtgaatatt atgggtctgc caacacatcc actaagcggc
     4441 agaacgccta cccggttatg cgtctggtgg ccttaatgaa cctgggaagt cacattctgc
     4501 tgaatgccgt gaccgcacct taccggcaga gcgaaactgt gctggcccac tccatgctcg
     4561 ccaccatccc ggataactct attacgttgt ttgataaact tttttacagc gaagacctgc
     4621 tgctgacgct gaaccagaag ggttgtaacc gccactggct actgcctgcg tggaagaata
     4681 tcgcatcaga aatgatagag ctgggaaaca cggcgtcacc agggaccatc ccgaaacgac
     4741 tggagcatct tcggggtgct ctggaagtgg tgttcataac aaaacgcccc cgaccatcaa
     4801 ggccgaggtc ggttaagata tcaaaaaccc gttatcctgt gaaacatagt gccgctcctc
     4861 ttaagtgaac ggcattgccc ttctggggcc gtattttttg tttacccaaa gcaacttttc
     4921 cataaaccga cagcattagc cttcatcata tttgcgacga tgtataacgc ctaaacacag
     4981 ggatattgta ctttacaggt cacaagtcaa cgtcggtgct taagagccct gtgaggcgta
     5041 tagcggcgtt aaaaaactgc cgagaagggt atatagcccg gaagaagtgc gtaaaacgaa
     5101 ctgacaggat aaaagtgccc tgctcaccct gtcagtaaag aaattcttat taatcgtggc
     5161 gatgcctttc ctgaatagcc gttaatgagc cgacttgtaa cgcctcttta tagtgtttac
     5221 ggcatacaga aacgtatcgt tcattaccac caattaccac ctgctcacct tcgttataag
     5281 gtctgcctgc ttgatcaaga cgcagcacca tgcttgcttt acggccacaa aaacagatgg
     5341 tttttaattc aaccagtttg tcggaccatg ccagtaagta ttggctgcca ataaataatt
     5401 cacctcgaaa atcggtacgt aaaccataac aaagtacggg tatatcgagt tgatcgacaa
     5461 cctccgataa ttcatatact tgttgtctgg ttaaaaactg gcattcatca accagtacgc
     5521 aatgaattgc ctgctgttca tgttccgcac gaatctcatc aaataatgat gaattttggt
     5581 taaataattt tgcaggcgat gacaaaccta tacgcgaact gactttcccg gcaccaaagc
     5641 gatcatcaat ttctgccgta tatacgacag tgcgcatgcc gcgttcctgg taattgtatg
     5701 aagattgcaa caatgctgta gacttacccg cattcattgc ggaatagtag aaatatagct
     5761 gtgccatcag ccacaggccc tcaatgatat gaaataaagt tggctggagt ttatcataat
     5821 tcgtcagttt ttcagctcaa cagtatgcac agaagtgggt atatgcgttc tcccttacga
     5881 agccttgcat aatccttctg agctatcatt acaactgcct cgcttgttat aagcgggtaa
     5941 atgactgctg gtaactattc acaatcttta acctgttgcg caagtaatag ccctctgttg
     6001 acctccagga gatagtgcaa tactaagtcc atgctcttat tgcgactgtt ctacttttca
     6061 tcattcgctt aatagggaat tctcgtaaac acaactaata cagaagactg aaaggtcgtc
     6121 agcctacgat aatctcccca taaaatgtga catgaatcag gaagttttaa cctcacgtgc
     6181 tgcgaaatca tcggtgtaaa tagggctata tgccgcgtct tttctggcta attttatgaa
     6241 aagatattta ttggcggcac aaaataaaga acaattttga attccttaca ttcctggcta
     6301 ttgcacaact gaatttaagg ctctattatt acctcaacaa accaccccaa tataagtttg
     6361 agattactac aatgagcgaa gcacttaaaa ttctgaacaa catccgtact cttcgtgcgc
     6421 aggcaagaga atgtacactt gaaacgctgg aagaaatgct ggaaaaatta gaagttgttg
     6481 ttaacgaacg tcgcgaagaa gaaagcgcgg ctgctgctga agttgaagag cgcactcgta
     6541 aactgcagca atatcgcgaa atgctgatcg ctgacggtat tgacccgaac gaactgctga
     6601 atagccttgc ttccgttaaa tctggcacca aagctaaacg tgctcagcgt ccggcaaaat
     6661 atagctacgt tgacgaaaac ggcgaaacta aaacctggac tggccaaggc cgtactccag
     6721 ctgtaatcaa aaaagcaatg gatgagcaag gtaaatccct cgacgatttc ctgatcaagc
     6781 aataatcttt tgtagattgc acttgcttaa aatcccgcca gcggcgggat tttttattgt
     6841 ccggtttaag acaatttaat aagaataagc tataaaaaaa cggcgtcgat tgctcaacgc
     6901 cgtttcgtgg ataacaccga tacggatgtt acttcttaat gcccatctct tcttcaagcc
     6961 aggctttaaa ttccgtgcca agggtgttat gacgaatacc gtattcaacg aaggcctgca
     7021 tgtaacctaa tttattaccg cagtcatggc tcttcccttt catatgatag gcttccaccg
     7081 tttctttttc gatcagcata tcaattgcgt cggtgagctg aatttcatca ccagctcccg
     7141 gaggggtttt tgccagcaac ggccaaatat ccgcgctaag tacgtaacga cccacaatag
     7201 cgagattaga cggcgcaaca tccgctttcg gtttttctac cacaccaacc atcggtacgc
     7261 tttcacccgg cgctaattca acgcctttgc aatccacaac gccatatgcg gtcacatcag
     7321 caaccggttc aaccatgatc tggctatgac ccgtttcatc aaagcggcgg atcatctctg
     7381 ccaggttatc ctgtgacaaa tcggattcat attcatccag aataacatca ggcaaaataa
     7441 cagctaccgg ttcatcaccc actaccgggt gagcacacaa taccgcgtgt cccaggcctt
     7501 tcgccagacc ctgacgaact tgcataatag tcacgtgcgg tggacaaata gactgcactt
     7561 catcaagcag ttgacgtttt acacgttttt ccagcattgc ttccagttca aaactggtat
     7621 caaagtggtt ttcaatagag tttttagatg agtgtgtaac cagcacaatt tcagtaatgc
     7681 cagccgcaat acattcattc acgacgtatt gaattaatgg cttatcgaca agtggcagca
     7741 tctctttcgg gatggctttc gtcgccggca acatcctggt tcctaatccc gcaacgggga
     7801 taacggcttt tttgactttc gtattaatgg cagccattta aattctcctg gactgttcgt
     7861 gttttgaacg tgttcatatt tctgtatcgc atcccagtat atcagcaccc cgaaggggct
     7921 aagttctgaa aaggaggcga gttagcataa ttaagacaat tacttatacg aatggcgacg
     7981 atagtaccac cagccgacat tagcaggtaa tgcaaattta gcccgcgtta tcgtttgctc
     8041 attctgcaga caacatcaag cgcagtcgac caccggctcc ccatatttgg cattgccagg
     8101 catcgcatcg ctggctcaat tgattcaaat aagcgttacc taaagtgcct aacggaacac
     8161 cattactgat ttgcacctgg tgttcgccgg tatttaacgt cgcattcaga cccgcagaaa
     8221 ccagaatgag atttttcagt tcgcgatgat aatagccaac taatagcgga aactgccccg
     8281 gcagattggc ctgacgaagt aaatggttta cctgcttcaa taacgcgccc aactctggca
     8341 accgttgatt ttggtgtgca agctgttcct gtaataatcc gttaaacaat gcgcgtaata
     8401 acaaggcagc aagtacgcca ttatgtccag ctcgggtgac atcaaggcaa taaaatgcca
     8461 gatcgttttc cgaaagtgcg gcaatatcaa gcaccaggcc gggtttgtcc gcggcaacca
     8521 attgacgata attaacccgg caatgggaaa tcacctgctg aaccggcggt tgtagttcct
     8581 gtaataattt cgccgctgcg gcagggttat caaccattgc atcccagtcg cgaaaaagcc
     8641 tttcctcttc ctcaacgcgc gaattaaaca tgctgggata gagacaggca aaaaccatct
     8701 cgcgcaagcg attcagatct ttaactggtt tcagcaaaac atcttcaacg cccagacgta
     8761 acgctttggc aatatctgcc atattttcgg tggcagatat caccagaacc ggtgtctggt
     8821 cacctctgtt gcgtatatgt tccagcagtt taagcccgtt cattcgtggc atcgcgatat
     8881 cacatatcat caggtctgga gtgaaacctc ccagcaactc aagggcatcc accccatcag
     8941 ccgccagtac cgttgtcgct cccaatgagg aaaaccacga atccagaagc gagcgaaata
     9001 ccggctcatc ttcaacaatg agaatctgtt ttccgaccaa tggctgcgtc atgttctctc
     9061 ccctgactgg ctttactcaa tagtgacatg ctattgccac ctgcgcctgt cagaattaac
     9121 ttaagtgtag ataaaaattc tggtcaaatg ttggtgcgta ccaacggcaa aagttcgtcc
     9181 attttctttt ccactgccag ctgtccggcc gcaatggcag cgtgcgcgcg atggaaatca
     9241 agcgtagata tttgcgggca aacaggttga atcagaatat cgggcggatc acctgccatg
     9301 cggttccttt taaggcggtt ctccagcacc tggatagaag tggtcataat ctctgttgcc
     9361 gttggcgctg tcaccgcccg acgtgtcgtt atgctgccta acctttcttt cagacgcgca
     9421 tgccacggca gagaatcacc attctcgcta ttttcttcac tgacattaaa ggagagcaaa
     9481 tcttgttgca tcaaatgagc atcgtgctgc aggtcaaccg ctatcacaat atcagccccc
     9541 aatgcacgcg tgagggaaat aggaattggg ttaacgactg ctccatcaac cagccagtag
     9601 ccgttatgtg caacaggtgc catgagtcct ggaatactgc atgatgcgcg aatagcaaga
     9661 tggagatcgc cttcagtaaa ccataattca cgtcccgtac ttaaattggt ggcaacagcc
     9721 gcaaagcgac gggaacaatt ttcgatctct gtttccggca ttatttcgcg gtattgattg
     9781 aagacacgct cgccgcgcag taacccaccg cgctgccagg agagatccat caggcgtaaa
     9841 acatcccaat aactgaaaga ggtcacccaa tcttccagcg cagataatcg atcgcatgca
     9901 taggcagcgc ccaccagcga accaattgaa catcctgcaa cgatatcaat ttcaataccc
     9961 acttttttta gcgcattaat aacgccaata tgcgaccaac ctctcgccgc gccagatccc
    10021 agcgccagcc ctatttttat ctttctcatt attcccgcaa gattcccctg gaatgcaatc
    10081 gtagccacat cgctactgct cggttaacat agtgctactc aggcggttaa tacgccgtta
    10141 ttgtttccag ggagattatt tgtgtctcag ctttgtccct gtggtagtgc tgtcgagtat
    10201 agcctatgtt gccaccctta tgtgtctggt gaaaaggttg cacctgatcc agaacatctc
    10261 atgcgttcgc gttactgcgc ttttgtgatg caagacgcag attatttaat aaagacctgg
    10321 catccctctt gtggagcagc ggcgttacgt gccgaattga tggccggatt tgcgcatact
    10381 gaatggttgg gattgaccgt atttgaacat tgttggcagg acgccgacaa tatcggattt
    10441 gtcagttttg ttgctcgatt taccgaaggt ggtaaaacag gtgcaataat tgaacgttcg
    10501 cgatttttga aagaaaacgg tcaatggtac tatattgacg gtacacgtcc gcagtttggt
    10561 cgcaacgatc cctgcccttg tggttcaggt aaaaaattta aaaagtgctg cggccaataa
    10621 tggttgacgg tacggtttag caaacactct caacaaggtt tttccagcaa tgcattcact
    10681 ccaacgtaaa gttctgcgta ctatttgtcc ggaccaaaaa ggtctgatcg cacgtattac
    10741 caatatttgc tacaagcacg agttaaatat cgtacagaac aatgaatttg ttgatcaccg
    10801 taccgggcgc ttttttatgc gcacggaact ggaagggatt tttaatgatt ccaccctgct
    10861 ggcggatctc gatagcgcat tgccagaagg ctccgtgcgt gagctgaatc ctgccggtcg
    10921 tcgccggata gtgattctgg tcactaaaga agcgcattgc cttggcgatt tgttgatgaa
    10981 agccaattac ggcggcctgg atgtcgaaat cgcggcagtt attggtaacc acgatacttt
    11041 acgttctctg gttgagcgtt ttgatattcc gtttgagctg gtaagccatg aagggttaag
    11101 ccgcaacgag cacgatcaaa agatggcgga cgccattgat gcttatcaac ctgactatgt
    11161 ggtgctggcg aagtatatgc gggtattaac gccggaattt gtggcacgct tcccgaataa
    11221 aatcatcaat attcaccatt cattcctgcc tgcgtttatc ggcgcacgtc cttatcacca
    11281 ggcctatgaa cgtggtgtga agattattgg cgcaaccgct cactatgtga atgacaatct
    11341 ggacgaaggc ccaatcatca tgcaggacgt tattcatgtc gatcatacct acacagctga
    11401 agatatgatg cgcgcaggtc gtgacgtcga gaaaaacgtc ttaagtcgtg cgctatacaa
    11461 agtactggca cagcgcgtct ttgtttacgg taatcggacg attattcttt aatcgccagc
    11521 aaaaataact ggttaccttt aatccgttac ggatgaaaat tacgcaacca gttcattttt
    11581 ctcaacgtaa cactttacag cggcgcgtca tttgatatga tgcgccccgc ttcccgataa
    11641 gggagcaggc cagtaaaaag cattaccccg tggtggggtt cccgagcggc caaagggagc
    11701 agactgtaaa tctgccgtca tcgacttcga aggttcgaat ccttccccca ccaccatctc
    11761 aagtattacc tcaaaatttg aattacccct ggtggggttc ccgagcggcc aaagggagca
    11821 gactgtaaat ctgccgtcat cgacttcgaa ggttcgaatc cttcccccac caccatcact
    11881 tttcaaaagt ccgtaaactc tcaaacgaat ccgcaattaa atattctgcc caagcgggga
    11941 aggatgagaa gcttcgacca aggttcgact tgagcgccag cgagagagcg ttgccgcagg
    12001 caacgacccg aagggcgaag cgcgcagcgc tgagtaatcc ttcccccacc accatcactt
    12061 tcaaaagtcc gtgaactctc aaacgaatcc gcgattaaat attctgccca tgcggggaag
    12121 gatgagaagc ctcgaccaag gttcgactcg agcgccagcg agagagcgtt gccgcaggca
    12181 acgacccgaa gggcgaagcg cgcagcgttg agtaatcctt cccccaccac catcacttcc
    12241 ttaaataatc aaaacctgtt actccgagct aattttgccc gatggcacca cgtttaccgg
    12301 gcctacaata tcctcccctg taggtcggat aagcgaagcg catccgacaa ctcaccccgc
    12361 accgtgccca taaaaaaacc ccgccgaagc agggtctatg tgaactaaaa ttcgcttagt
    12421 gacgagcgcg caccagctgg tacttacgtg tcagatactc caccggtacg ctccagatgt
    12481 gtaccagacg cgagaacggg aacagcagga ataacgtcat ccccagcacc aggtgcagac
    12541 ggaagataaa cgccacacca tcgaggtgtt gagaagcgcc accgtggaag gtcaccaccg
    12601 actgcgccca gccaaccagt ttcatcatct cgctaccgtc catatgctga gcggagaacg
    12661 gaatggtcaa caggcccagc gcgcactgga taacgagcag cgacaggatc aggatatccg
    12721 ctccggtagt ggttgcacgc acgcgtgggc tgaacagacg acgcttcagc aacagcacgc
    12781 cgccaatcag acacagcacg ccgctggcac caccagcaaa catcgccatt ttctgtttca
    12841 cttcaatcgg cagccaggct tcatacatcc agtgcggcgt cagcataccg aagaagtgac
    12901 caacaaaaat ccccagaata ccgatatgga acaggttcga cgccaggttc atccctttgc
    12961 gatccagcat ctggctggac gccgcgcgcc aggtgtactg cccgtagtca taacgcagcc
    13021 agctaccaat caggaagacc gccccggcta tgtacgggta gatatcaaag aagaacatat
    13081 tcaggaattg cattagtgct gtcctccggt ggtgatattc agatattgcg gcgcgacggc
    13141 accggcaaag cgacgctgat gagcagtgat tgctgaatca ccgcagcctt tgtcagcaaa
    13201 gaatttaacc tgctcttctt cccagacagc atccagcgcc tgcggcgtat catcgcgcgc
    13261 ttcgtcggca attttttccg ccactttgtc gctgtcgata gcggtattgg ccaatttcag
    13321 cagcagatca aacatcacgc cataacggct ttcacgctgt tgcagacgcg cgctcagcaa
    13381 tgccagaatc ggcgcgatat ctttcaaacc ttccacggct tcgctttgcg gcagctgcga
    13441 caggtactcc agatacagcg gcagatggtc cggtaattcg cggctgttta actgcaagcc
    13501 gtgctgctcg tactgcgcca gcaggtccac catcgcctgg ccgcggtcac gggattcacc
    13561 gtgaacatgt tcaaatagca acagtgacgt ggcgcggcca cggtcgaaca gttcgctgta
    13621 ctgggcctgg gcatcgagcg gatccatcgc cgttaaatcg cgcaggaaaa tgcccagcgc
    13681 atgggcatct tctttcgaca gatttttcga cgcagcaatc gcctcaaaca tctcttgttg
    13741 atgctgccat aaggcagcat ccggatattc aaggagacgc gatacaatca cgagttcgat
    13801 catggatgcg gctccgtttt gctggtcaca tcgatggcat cgatacgacg gctgttgaac
    13861 agattgaatt tggtatctga accgtggcag ccatcaccaa aggtaaagcc gcagccattt
    13921 ttctccggga aggcttcccg tgccagttca cgatgactac tcggcaccac aaagcgatct
    13981 tcgtagttag caatcgccag ataacggtac atctcctgtg cctgggcttc ggtcagaccg
    14041 acctcttcca gcgcacgggt atcaacttta ccgtcaacgg tttcagcacg tttgtaatga
    14101 cgcatcgcca acatacgttt cagtgcgcgc agtaccggtt tggtatcacc ggcggtcagc
    14161 agattcgcca gatactgtac cggaatacgc aagctttcga cgtctggcag aatgccgttg
    14221 ctacccagct cacccgcgtc tgctgcagac tgaatcggag acagaggcgg cacgtaccag
    14281 accatcggca gtgtgcgata ttctggatgc agcggcagcg ccagtttcca ttccattgcc
    14341 attttataaa ccggcgactg ctgtgcggct tcaataacgc tcagcggaat accgtcttta
    14401 atcgcctgct cgatgacttt cggatcgttc ggatcgagga acacctccag ctgacgctgg
    14461 taaagatctt tctcgttctc ggtgctggct gcacgttcaa tcgcgtcggc atcgtacagc
    14521 agcacgccaa gataacggat acgaccgaca caggtttctg agcacacggt cggctgaccc
    14581 gcttcaatac gcggatagca gaagatgcac ttctcagact taccgctctt ccagttgaag
    14641 tagatttttt tgtacgggca tccggtgatg cacatacgcc agccacggca tttatcctgg
    14701 tcgatcagga cgatgccatc ttcttcacgc ttgtaaatcg caccgctcgg gcaggtcgcc
    14761 acacatgccg ggttcaggca gtgttcgcac aggcgcggca aatacatcat aaaggtgttt
    14821 tcgaactggc tatacatcgc cttctggatg ttgtcgaagt tcttgtcttt cgccagtttg
    14881 tcaaactcac cacccaggtc atcttcccag ttcggccctt tttcgatttt cgccatccgt
    14941 tccccggtaa tcagcgaacg cggacgggca atcggctgcg atttgctgcc ttccggcgcg
    15001 gtatgcaggt tctgatagtc aaaatcgaac ggctcgtaat aatcgtcgat ccccggcaga
    15061 tgcgggttag cgaagatttt acccagcaac atggcacggt tacccatgcg cggctgcagt
    15121 ttgccgttga ttttacggat ccagccgcct ttgtattttt cctggttttc ccagtcagtc
    15181 gggaagccct gacccggctt ggtttccacg ttgttgaacc acgcgtattc cacgccttca
    15241 cggctggtcc agacgttttt acaggtaact gaacaggtgt ggcagccgat gcacttatcg
    15301 agattcagca ccatgccgac ttgtgaacga attttcattt tacgctctcc tgtacctggt
    15361 cattgccttc gccatctaac cagtcaatgt tcttcatctt acgcactaca acaaactcat
    15421 cgcggttaga acctacggtg ccgtagtagt taaagccgta tgccagatgg gcatagccgc
    15481 cgatcatatg cgtcggtttc ggcgtgatac gggtgaccga gttatggata ccaccacgct
    15541 gttgggtaat ttccgaacca ggcaggttaa cgatacgttc ctgcgcgtgg tacatcatgg
    15601 tcatccctgc cggaacacgc tggctgacaa ccgcacgggc agtcagagca ccgttgctgt
    15661 tgaagacttc aatccagtcg ttatcggcga tacccagatc tttggcatcg gcttcactca
    15721 accagaccac cggaccaccg cgacctaaag tcagcatcag caggttgtcg ctgtaggtgg
    15781 agtggatacc ccacttctgg tgcggcgtca ggaagttgag cgctttttcc gggttgccat
    15841 tggatttctg gcccatcact tctttcaccg aacgggtgtc gatcggcgga cgataaacca
    15901 gcaggctttc accgaaatca cgcatccatt ggtgatcctg atacagctgc tgacggccag
    15961 agagcgtacg ccacgggatc agctcgtgaa cgttggtgta accggcgttg taagaaacgt
    16021 gttcatcttc aaggccagac caggtcgggc tggagatgat tttgcgcggc tgtgcctgaa
    16081 tatcgcggaa gcgaatcttc tcgtcttctt tattcagcgc cagatgcgta tggtcacgac
    16141 cggtaaactc gctcagggca gcccaggctt tcacggctac ctgaccgttg gtttccggtg
    16201 ccagcgtcag gatcatctct gccgcatcaa ttgcggtgtt cagcatcggc tggcctttcg
    16261 ccggaccttc cgctttggtg tagttgagct tacgcagcag atccatctcg ctctgggtgt
    16321 tccaggcaat ccctttaccg ccattaccga ttttctccat cagcgggccg atagaggtaa
    16381 agcgttcgta agtcgccgga taatcgcgct ctacgaccat aatgtgtggc gcggttttac
    16441 cagggatcag gtcgcactcg ccttttttcc agtctttcac atccagcggc tgcgccagtt
    16501 cagcggcaga gtcgtgctgg ataggcagcg tgacgatgtc ggtttcttta cccagatgac
    16561 cgacgcacac ttcggagaat ttcttcgcga tggctttgta aatttcccag tcgcttttcg
    16621 cttcccaggc cggatcgacc gccgcagaca gcgggtgaat aaacggatgc atatccgaag
    16681 tattcatgtc gtctttttcg taccaggtcg ccgtcggcaa aatgatgtcg gaatagagac
    16741 aggtgctcga cagacggaag tccagcgtaa ccaccagatc cagcttgcct tccagaccat
    16801 tgtcctgcca gtccacttct tccggcttca cgccgccctg ttgccccaga tctttaccct
    16861 ggataccgtg ctccgtcccc agcaggtact tgagcataaa ctcatgacct ttaccggaag
    16921 aaccgagcag gttagaacgc cagatgaaca ggttgcgcgg gtggttttta ccgttttctg
    16981 gttgttctgc cgcaaaacgg atggaaccct ctttcaggga tttcaccgta tagtccaccg
    17041 gattcatccc ggctttttcc gcttcgccag cgatagtcag cgggttagtg cctaactgcg
    17101 gtgcagacgg cagccagccc atgcgttccg cacggacgtt aaagtcgatc aagtgtccgg
    17161 tatagcggga tttgtccgcc atcggtgaca gcaactcttc cgccgtgacg gtttcataac
    17221 gccactggct ggagtggtta tagaaataag aagtgctgtt catgtgacgc gccggacgct
    17281 gccagtcaag ggcaaacgcc agcggctgcc agccggtttg cggacgcagt ttttcctgac
    17341 ctacatagtg cgcccagccg cccccgctct gaccgacaca gccgcagaaa atcagcatgt
    17401 tgatcagacc acgatagttc atatcgaggt gataccagtg gttcagcccc gcaccgacga
    17461 taatcatcga acgaccgtgc gttttatcag cgttatcggc aaattcacgg gcgatgcgaa
    17521 taatctggct gcgagaaacg ccggtaatct gctcggccca ggccggggta taagctttca
    17581 catcgtcata gctggttgca cagttaacgt cgttcaggcc acgttccaga ccgtagtttg
    17641 ccagcgtcag atcataaacg gtggtcacca gggcggtgct gccatcagcc agttgcaggc
    17701 gtttcaccgg cagtttgtgc agcagcacgt tttccagttc cactttgttg aagtgttccg
    17761 tgccgtcgcc accaaagtac gggaagccta cctcagcgat ctcatcctgg ctacccagca
    17821 ggctcagttg cagctcggtt tcttcgccag ttttgccgtc gcgctgctca agattccatt
    17881 tgcccttctc gccccagcgg aagccaatag aaccgttcgg cgcaaccatt tcgccattgg
    17941 tattaaaggc gacagttttc cattccggat tgttttcctg gcccagcgca tcaaccagat
    18001 cagcagcgcg cagcatacga cctgcagcgt agtaaccgtc gcgttcttcc agcatcacca
    18061 gcatcggcat gtcggtgtag cgacgcacat agtcggtgaa atactggctt gggttgtcga
    18121 ggtggaattc acgcagcatt acgtggccca tcgccagcgc cattgccgca tcggtgccct
    18181 gtttcggtgc cagccacaga tcgcacagtt tggcgatttc agcgtagtct ggtgtgacgg
    18241 caacagtttt ggtccctttg taacgcactt cagtaaagaa gtgagcatcc ggggtacgcg
    18301 tctgcggtac gtttgacccc caggcgatga tgtagctgga gttgtaccag tcagcagatt
    18361 ccggtacgtc agtttgctcg ccccaggttt gcggagacgc aggaggcaag tcgcagtacc
    18421 agtcgtagaa gcttaagcaa gtaccgccaa tcagcgagag atagcgtgca cccgatgcgt
    18481 aagaaaccat cgacattgcc ggaattggcg agaaaccagc aacacggtcc gggccgtagt
    18541 ttttgatggt gtaaacgtta gatgcggcga tcagttcgtt cacctcctgc caggaagaac
    18601 gaacaaatcc accgcgtcca cgcgcctgct taaagctttt cgctttatcg gcgtcttcaa
    18661 tgatagaagc ccatgcctca accggatcgc tatgcagcgc cttcgcttca cgccacattt
    18721 tcatcaggcg tttgcgcatc atcgggtatt tcaggcggtt ggcactgtaa agataccagg
    18781 agtagctggc accgcgcggg cagccgcgag gttcatggtt tggcagatcc ggacgggtac
    18841 gcggatagtc agtctgctgg gtttcccagg tgaccagacc gtttttgacg tagattttcc
    18901 agctgcagga gccggtgcag tttaccccgt gggtagagcg gacgattttg tcatgctgcc
    18961 aacgctggcg atatccatcc tcccagtcac ggttggtatt gagaagctgg ccatgcccat
    19021 cggcaaaggt ttcacccttc tgtttgaagt agcgaaaccg gtccaggaat ttactcatcg
    19081 gttttctcct gtgggagcct gtcggccctc tgataaatcg acattgctaa tgtttaaggg
    19141 caaggtaacg ctctgaaaca gcgtgggaat tgataacgat caagtgtgat ggggatgaaa
    19201 aataaagtaa attcctaaga taccccttta ggataattaa cagttaaagc ataacatgct
    19261 gtaaattaag acgttttata cctgtagtta tttaaagcat gaagattttt atgcagatgg
    19321 gtattaagga gtattcccca tgggtaacat attaatggct cttaatgaaa tccggcgaat
    19381 aagc
//
LOCUS       DAZGTR010000084        19040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGTR010000084 DAZGTR010000000
VERSION     DAZGTR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.084"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..283
                     /locus_tag="ACHLCY_003278"
                     /pseudo
     CDS             1..283
                     /locus_tag="ACHLCY_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            459..1049
                     /locus_tag="ACHLCY_003279"
     CDS             459..1049
                     /locus_tag="ACHLCY_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFP0370473.1"
                     /translation="MKIISTVIQTPFPFENNNSHTGVVTEPILGKLIGQGSTAEIFED
                     MNDSSALYKKYDLVGNQHNEVLEMARQESALFNTFYGDDASVVIQYGGDVYLRMLRVP
                     GIPLSDIDTADIPDNLESLYLQLICKLNELSIIHYDLNTGNMLYDKESNSLFPIDFRN
                     IYSEYYSATKNDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1088..1305
                     /locus_tag="ACHLCY_003280"
                     /pseudo
     CDS             1088..1305
                     /locus_tag="ACHLCY_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1415..1678
                     /locus_tag="ACHLCY_003281"
                     /pseudo
     CDS             1415..1678
                     /locus_tag="ACHLCY_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            1901..2026
                     /locus_tag="ACHLCY_003282"
                     /pseudo
     CDS             1901..2026
                     /locus_tag="ACHLCY_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(2086..2592)
                     /gene="yciE"
                     /locus_tag="ACHLCY_003283"
     CDS             complement(2086..2592)
                     /gene="yciE"
                     /locus_tag="ACHLCY_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0370474.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2638..3138)
                     /gene="yciF"
                     /locus_tag="ACHLCY_003284"
     CDS             complement(2638..3138)
                     /gene="yciF"
                     /locus_tag="ACHLCY_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0370475.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(3224..3403)
                     /gene="yciG"
                     /locus_tag="ACHLCY_003285"
     CDS             complement(3224..3403)
                     /gene="yciG"
                     /locus_tag="ACHLCY_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0370476.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(3784..4590)
                     /gene="trpA"
                     /locus_tag="ACHLCY_003286"
     CDS             complement(3784..4590)
                     /gene="trpA"
                     /locus_tag="ACHLCY_003286"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP0370477.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(4590..5783)
                     /gene="trpB"
                     /locus_tag="ACHLCY_003287"
     CDS             complement(4590..5783)
                     /gene="trpB"
                     /locus_tag="ACHLCY_003287"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP0370478.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(5795..7156)
                     /gene="trpCF"
                     /locus_tag="ACHLCY_003288"
     CDS             complement(5795..7156)
                     /gene="trpCF"
                     /locus_tag="ACHLCY_003288"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP0370479.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(7157..8752)
                     /gene="trpD"
                     /locus_tag="ACHLCY_003289"
     CDS             complement(7157..8752)
                     /gene="trpD"
                     /locus_tag="ACHLCY_003289"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP0370480.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(8752..10314)
                     /gene="trpE"
                     /locus_tag="ACHLCY_003290"
     CDS             complement(8752..10314)
                     /gene="trpE"
                     /locus_tag="ACHLCY_003290"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP0370481.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGMENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNMLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTETAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(10336..10429)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(10406..10450)
                     /gene="trpL"
                     /locus_tag="ACHLCY_003291"
     CDS             complement(10406..10450)
                     /gene="trpL"
                     /locus_tag="ACHLCY_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP0370482.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            10588..11469
                     /gene="rnm"
                     /locus_tag="ACHLCY_003292"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             10588..11469
                     /gene="rnm"
                     /locus_tag="ACHLCY_003292"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP0370483.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11466..12086
                     /gene="yciO"
                     /locus_tag="ACHLCY_003293"
     CDS             11466..12086
                     /gene="yciO"
                     /locus_tag="ACHLCY_003293"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP0370484.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            12187..13059
                     /gene="rluB"
                     /locus_tag="ACHLCY_003294"
     CDS             12187..13059
                     /gene="rluB"
                     /locus_tag="ACHLCY_003294"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP0370485.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(13099..13689)
                     /gene="cobO"
                     /locus_tag="ACHLCY_003295"
     CDS             complement(13099..13689)
                     /gene="cobO"
                     /locus_tag="ACHLCY_003295"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP0370486.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(13686..14444)
                     /gene="yciK"
                     /locus_tag="ACHLCY_003296"
     CDS             complement(13686..14444)
                     /gene="yciK"
                     /locus_tag="ACHLCY_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP0370487.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFLLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            14664..15713
                     /gene="sohB"
                     /locus_tag="ACHLCY_003297"
     CDS             14664..15713
                     /gene="sohB"
                     /locus_tag="ACHLCY_003297"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP0370488.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(15749..16000)
                     /gene="yciN"
                     /locus_tag="ACHLCY_003298"
     CDS             complement(15749..16000)
                     /gene="yciN"
                     /locus_tag="ACHLCY_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP0370489.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            16380..18977
                     /gene="topA"
                     /locus_tag="ACHLCY_003299"
     CDS             16380..18977
                     /gene="topA"
                     /locus_tag="ACHLCY_003299"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP0370490.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         4745 a         4657 c         4907 g         4731 t
ORIGIN      
        1 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
       61 tcgtggtatg gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgt
      121 gcacgttatc agcaagcgtt acactcagcg cattgagcga cataatctga atctgagaca
      181 acacctggca aggccgggac ggaagtcact gtcgttctca aaatcggtgg agctgcatga
      241 caaggacatc gggcattatc tgaacataaa acactatcag taagttggag tcattaccta
      301 aaggcctctc tacactgtgc ttgtatgggg cttccctgat catggcggtt atctatgtaa
      361 tagcatatgt acttacgtgg tttctgaggt ggtagcgcgt aatattattt tatgtaaaaa
      421 tatcttgtaa tctttaaatt tcataacaag ggcatgtcat gaaaataata tccactgtta
      481 ttcaaacacc tttcccattt gagaataata attctcatac tggcgtagta acggagccta
      541 ttctcggtaa gttaataggg caaggttcaa cagcagaaat ctttgaagat atgaatgatt
      601 cgtctgcttt atataaaaag tatgatcttg ttggtaacca gcacaatgaa gttctggaaa
      661 tggctaggca agaatctgcc ctttttaata ctttttatgg cgatgacgca tcagttgtta
      721 tacagtatgg cggtgatgtg tatctccgaa tgctacgcgt gccagggatt ccccttagtg
      781 atattgatac agctgatatt cctgataatc tagagagcct ttatctgcag ttgatatgta
      841 aattgaatga gttgagtata atccattacg atcttaatac aggtaatatg ttgtatgata
      901 aagaaagtaa tagtttattt ccaatagatt ttcgaaatat ttattctgaa tattactctg
      961 caaccaaaaa tgataaagag attatcgatc gacgattaca gatgcgtaca aatgattttt
     1021 attcattatt aaacagaaaa tatttataag tgctatattc atgaatgaaa aatagctata
     1081 tgcgtacatt ctcaacctgc ccgtcacgta gcaggttgaa tcactttttc ttgataaagg
     1141 gctggtcctg ccaccgttgt tgttgggtgc gttaaaaata cactactaat atgcccggca
     1201 tgcgggcaac agagtcctgt tcacgatcgc cgccaccgga aatggcgctg cctcgatacc
     1261 tgccagttca tggcactatt cgaagccgat gttccccacg ttatatggag taatcattgg
     1321 ggttatgccg cagcacgttg tatgcaagaa tgtgctgcgg ctggtttgtg agctttcgat
     1381 agtggttgtt atttttgccc ttatttgttc cggaggccct ggttcaatgg tccgtctgcc
     1441 ccctgtggtg atgtcagcaa aatcagccac tgcgcgaacc acaatagccc gggaagatgc
     1501 tgaagatcac caggtaaagc tgtcagcgca gaaactggaa gaactgctcg catcaatggt
     1561 taaggatgag gttgatcgca atgatgggat ttattgacgt cagcgagagc agaaggaaga
     1621 actgaataac ctgaatgatt tacgctcaat cagagcgatg atgattggca gtaattagcg
     1681 gtcaatttcg ggttttttgg ctgtgcgctc cccgcccagc attgcgcgtc aattagcccc
     1741 aaaaaaaacg aaaaagttac aatcaagtaa aaaattcctt ctgcaaaact aaaaaagatt
     1801 tcaataaaat caggcagtta aaaacataaa aaaaagtaca aaaaagcatc tcaaagtctt
     1861 gttttttgct tcaaatacaa ttggttattg tgttttttga aacctaccag tgtggtacat
     1921 ggatatcgat accaccgcca attataagct aggcggtgca cagcaacacg atagcgtacg
     1981 cctcgatccg tgggtgttta tgttctcagc aggatatcgt ttttaattcc gcacaaaaac
     2041 gaccccgtaa tatacggggt caataaggac atggtataaa gggtattatt tcttcgcttc
     2101 tacgccatca gtttcagaac gaattaaaaa tttctcagtt gtttgcggaa tatgctgaat
     2161 cagccagtcg gccatttgct tttcctcatt taaaatcgct tcgatgatcg gaattgagac
     2221 tgtatcaccg gcattttttg ctgctgctaa tagtgaggtg taacaggcga tttcaaattg
     2281 ctcgaagaca tatccgctaa tagagccttt aactatttca tcagaaggga atataccacc
     2341 gattgactgc ccaagcgcag ccattttact catggaatct ttaatgactg aacgtgaaat
     2401 gtcattacga tcaagaatag tttccagttg aacaatctgg tttttggttt cactaagatg
     2461 ttgttcaata cgagcgcgta gttcaggata attatctata cggctggcca tggattcaag
     2521 catagattcg gcttgctttt ccattgcgtg ggcgtcacgt aaccagtcat gataatgttc
     2581 aatacgattc atgaaaatgt cctctttata aaatgactaa aagttaaaat tcatatttca
     2641 ggctttattt tcggctttct tatttacgtt attaagggcc agatcagtca gtttgatgtc
     2701 ggtggccttt tcttcttcca gggtttcttt cagaagcttc gctgctttac ggtaacctaa
     2761 ttgttcagcc agcgtcgcta atgtcccgta actggcaatc tcataatgct cgactttctg
     2821 cgctgcggca atcagtgcgg catcacgcac ttcgtttttc tcggtacttt cgatgacctc
     2881 attagcttct tcaataagac cttccattgc cacacatttc atgcgcttaa ttttcaggtt
     2941 cgattcggac tccacaactt gatcaatacg ttcaatctgt ccatgagttt cctcgaggtg
     3001 cgcatgaaaa gcctgactta atttttcatt tgatgttgct cttgcgagtt ttgccagtgc
     3061 ccgggttaat tgtttttctg cgctgtaggt atctgaaagc aggtgaataa atacatcttc
     3121 aatggtcttc atattcatat ttttctccag tgaaatcact gcgagccata cggcccgcag
     3181 atgcaattat ctttatcagc tgatattaat aattaacggt gaatcaggat ttgcctgatt
     3241 tattaccacc gctttgttga ccgccttttt tacccgcttc agatgcgcgt tgcggatcat
     3301 ttttaaaatt accgccgcta tgctgaccgc ctttacggcc tgcgtcggat gccttctcac
     3361 ggtcttcggc gaaatttcct gaaccaccac gatgttcggc catgttattt ctcccgttgc
     3421 gttgcattgt ttcattaata tgagtgttgt gtgtcgacac tcattaaaat tagtcgctaa
     3481 tgagaattag tcaaattaag cgcaacgaga agatagagga aaaatatatt ttgaggaaca
     3541 ttctggatat attaacaatt acctgaggaa taagtgactt agagaaaatt attgatttta
     3601 ctggtgttat gttgcgggat taatttgttt gattatatta ctgttgggcg gaaaaatgac
     3661 ctaagttgac gttcgaccgg ggtaagcgaa acggtaaaaa gataaatatt aaatgaattt
     3721 aggatttttc cggcttcatt aaagaaagtt aaaatgccgc cagcggaact ggcggctgtg
     3781 ggattaactg cgcgtcgccg ctttcatcgg ttgtacaaaa actttcagtg ccgccagcat
     3841 tttctctggc tcattaatat gttgctcgat gattttaaca atggccgaac cagaaatcgc
     3901 gcccgcagct cctgcatcaa tcgctgcttt tacctgatcc ggggcggaaa taccaaatcc
     3961 ctgcaatgga ggtgcagcgt tgtactcttt cagcttcgca accagatgat tgaggggtaa
     4021 cgcggcgcgg ttttctgcgc cggtcacgcc tgctcgtgac agcaaatagg tgtaaccacg
     4081 accgtaagag gctatctggc gcagcaggtc gtcatcggca tttggcgggc agatgaagat
     4141 aggtgcgaca ttatgacgca acgcggcctg gcggaagggc gcggactctt caactggcac
     4201 atcggcaacc agcaccgaat cgacgccgac tttttcgcac tgggcataaa actcatcaat
     4261 gcctttgtta aacaccagat tggcatacat caacaggcca atgggaatgg tcgggtgttt
     4321 ctggcgaatc agtgccagca tttcaaaaca ttgtgccgga gtcacacctg ccgcaaaggc
     4381 gcgcagagtg gcgttttgaa tcgtcgggcc atccgccagt gggtcggaga aggggatacc
     4441 taactccagc gcgtcagcac cggcttcaat tagcgtatcg ataattttca atgactgctc
     4501 aatgcccgga tcaccgagcg tgacgaaagg aacgaatgcg ccttctttgc gctccttcaa
     4561 ctgggcaaac agagattcgt agcgttccat cagatttccc ctcgtgcttt caaaatatcg
     4621 tgaacggtga agatgtcttt atcgccgcga ccggaaaggt taaccaccag tagctgctct
     4681 ttatccgggt tttcgcgcat cattttcaac gcatgggcca gggcgtggga ggattccagc
     4741 gccgggatga tcccttcgtg caggcacagc gttttgaagg cttcaagggc ttcatcatcg
     4801 gtaatagaca cgtaatcagc gcgtccagtg ctgttaagat acgcgtgttg tgggccgaca
     4861 gacgggaaat ccagtccggc ggagatggag taagattctt caatctgccc gtcttcggtt
     4921 tgcatcatcg gcgctttcat accgaaatag atacccacgc gaccatgttt tagcggtgcg
     4981 ccgtgctcgc cagtttcgat accgtgacca cctggctcca caccaatcag gccgacgttg
     5041 gtttcattga tgaaatcagc aaacatgccg atggcattcg aaccgccgcc aacacaggcg
     5101 ataacggcat ccggcaggcg accttctctt tccagaatct gcgctttggt ttcttcgcca
     5161 atcatccgct gaaactcacg cacaatggtc ggataaggat gcgggccagc tgcggtgccc
     5221 agcatatagt gcgcggtttc gtaactaccg gaccagtcgc gcagcgcctc gttacaggca
     5281 tctttcagcg tcgcggaacc gctatgcacc gggatcactt ccgcacccat taagcgcata
     5341 cgaaaaacgt taggcgactg gcgttcaacg tctttggcac ccatataaat acggcatttc
     5401 aggccgagca gggcgctggc aagggccgac gccacgccat gctgaccggc accggtttcg
     5461 gcgatgattt cggttttacc catccgcttc gccagcaacg cctgccccag cacctggtta
     5521 gttttatgcg cgccgccgtg cagcaaatct tcacgcttga gatacagcgt ggtgttcgtc
     5581 ccggctgtaa tgttctggca tttggtcagc gcggttggac gcccggcata gtttttcagc
     5641 aggtcgttga actgagcctg aaattcagga tctttttgcg cactgacaaa agcttcttcc
     5701 agctggcgca gagcaggcat caggatttgt ggcacgtaca tgccgccaaa ctcaccaaaa
     5761 taggggttaa gtaatgttgt cattgttcct ttccttaata tgcgcgcagc gtctggaaaa
     5821 ccgaggccaa aagacgtgcg tctttgatgc ccggttgcga ctctacagca gaattaaaat
     5881 caagtccggc gcagccggtt tgtgccgctt ccacgcagtt atctgcgcct aagccccccg
     5941 ccagcagaac gttgccaagc gattgaccat ttaatagtga ccagtcaaaa cgttgcccgc
     6001 ttccaccctg gccgttgtct aaaacatatt tatcaacgtg ctgaaactcg cgggcgggca
     6061 gggtttcacc gacgcttaat gctttccaga tggcaacatg tgctggcaga gcttcacgca
     6121 gcgtatcgat atacagctgt tcttcattac catgcagttg cactgccgcc agcgataaca
     6181 ccttagcttt gtccaccaca tcggcaatat cgtgattgcg gaacacgcca acatactgca
     6241 acggtgccgc agccatcact tcctgcgcct gttcaacgtt gacgcaacgc ggtgatgtcg
     6301 caacaaaaat caacccaccg taaatcgcgc ccgcgtcata agctgcttta gcatcttgcc
     6361 cacgcgtcag gccacatact ttattctcac ccagcaacac ccggcgcacg gcggcgtgca
     6421 aatcgtcatg ggccatcaac gccgaaccaa tcagaaaacc gttagcgaag tggcttaact
     6481 cgcgcacctg agcgtaagta ttgatgccgg attcgctgat taccgtcacg ttgtgcccca
     6541 gtttcggcgc aagctcgcgg gtacggttga gatcaatcga caaatcacgc agatcgcggt
     6601 tgttgatgcc aacgaccttt gcccccaatg caatggcacg ctccagttcc tcttcattac
     6661 tgacttcggt cagcacaccc atctccagac tgtgggcgac ggcggcaagc tggcgatatt
     6721 gttcgtcatc cagtactgaa agcattaata agcaggcatc agcctggtaa tagcgcgcca
     6781 gatagatctg gtaaggatcg ataatgaagt ctttacataa aatcggctgc ggggcgattt
     6841 ggctgacgat ggggaggaaa tcaaagctcc cctgaaaata tttctcatca gtcagcaccg
     6901 aaattgccga agcgtaatgt ttataaatgg cggcaatgcg tgctggatcg aaatcatcac
     6961 ggatcacgcc ttttgacggc gacgctttct tgcactccag aataaacgcc gtgcgcgcac
     7021 cctgtagcgc atcataaaaa tgtcgcgtgc tcggctgaac ctcattctga aaactggcca
     7081 gcggttgctg ctgtttgcgg gtttctaccc aaatcgcctt gtctgcgacg attttcgcta
     7141 aaacggtttg catcatttac cctcgtgccg ccagtgcggt aactctgtcg taagcggaac
     7201 cactgcgcag tacctcaaga acggtttgcg cattggcttg cagatcttca tggccatgca
     7261 ggcgcattaa catggcgacg ttcgcagcga cggctgcttc atgggcggcg tcgcctttac
     7321 cttgtaacaa gcgtgttaaa atgtcacggt tttcttccgg tgttccgcct gccagttgct
     7381 cctggtggta gggagtcagg ccaaaatctt cagcggtcaa ttgatagctc ttaatttcgc
     7441 cgtcatgcag ctcggcaacg attgtcggcg cgtgtaatga aacttcatcc atcccgccgc
     7501 tgtgcaccac cgccgcgcgt tgatacccca gcacgcgcaa ggtttcggca atcggcagca
     7561 ccagttccgg actataaaca ccaattaacg ccagcggcgg atgcgccggg ttaatcaatg
     7621 gccccagcac attgaacagg gtgcgggttt tcagttgctg gcgaaccggc atcgcgtggc
     7681 ggaatccggt gtgatacttc ggcgcaaaga ggaaacatac acctaactca tccagcgcct
     7741 ggcgcgattt atcggcgttc atatcaagat taataccgaa cgccgccagc agatcggacg
     7801 aaccagattt actggagacg ctacggttgc cgtgtttcgc cactttcagc ccacaggccg
     7861 cggcgacaaa cgcactggcg gtagaaatat tgatactgtt gctgccgtca ccgccagtac
     7921 cgacgatatc agcaaacaga taatccgggc gcgggaacgg cgcggcgttt tccagtagcg
     7981 cggttgctgc tccggcgatc tcgttcgggt gctcaccgcg aattttcatg ctcaccagcg
     8041 ccgccgccag ttgttccggc ttcagctcgc cacgcaccac cgctgaaaac agctggtggc
     8101 tttcttgttg gctaagcgtc tgcgcctgat acagtttttc cagaatcggt tgcagcgtgt
     8161 tggctggctc tagtttctgc tgcgcccagg ccagcgtttg ttccagcagg cgagcgccct
     8221 gggtggtgag aatggattcc ggatggaact ggaatccaca aacgcgatcc gcatcgtgac
     8281 gtactgccat caccatgcca ttaaaatggg cgttgatggt taaaccggcc ggaatgttac
     8341 tgccaaccag cgagtgataa cgcgccaccg gcagcgggtt tgttaatccg gcaaacatcg
     8401 cctgaccgtc atgttcaatg ctcgacgctt taccgtgaag aatttcgccc gcctgaccga
     8461 catagccccc gtaagcttcg acaattgcct gatgtccgag gcaaatgcca ataatgggca
     8521 gcttgccacg caagcgggtg aggagttccg gcatacaacc ggcttcgctc ggcacaccgg
     8581 ggccaggaga aagcatcagt accggattgc tcatcgtcgc caggcgttca attaaggtct
     8641 gcgccggaat atggttgcgg taaatcacca cattatgccc attgctgcgc aactgatctg
     8701 ccaggttgta cgtaaaagag tcgatattat cgagcagcag aatgtcagcc atcagaaagt
     8761 ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca gcgcgggctt tattacgggt
     8821 ttcgtcggct tccgactgcg gaacagaatc aaggactacg ccagcaccgg cttgcacggt
     8881 ggcgataccg ttttccacca gcgccgagcg gatcacaatg caggtgtcga gatcgccatg
     8941 cgcggtgaaa taacctaccg cgccgccgta gctgccgcgg cgacgacctt ccgcctcggc
     9001 aattaactgc atagcgcgta ctttcggcgc accacttaac gttcccatat tcatacaggc
     9061 gcggtaagcg tgcagggcgt cgagatcgtg gcgcagctca ccgaccacgc gggagaccaa
     9121 gtgcatcacg taagagtaac ggtcaacttt ggtgaggtcg gcgacgtagc ggctgccggg
     9181 ggtgcaaatg cgtgccagat cattacgggc gagatccacc agcatcagat gttcagaaag
     9241 ctctttatga tcggtacgca tttccagttc gatgcggctg tcgaggtctc tgtccagtga
     9301 accatcggca cgacgaccgc gcggtcgtgt tccggcaatc gggtagatct caatctggcg
     9361 gctggtggcg tcatacttga gcgaactttc cggcgacgcg ccaaacaggg tgaaatcatt
     9421 atcctgcata aaaaacatgt acgggctggg attactcttt ttcagcacgt aataggccgc
     9481 cagcggtgac gggcagggca gagagaaacg gcgagatggc accacctgga aaatttctcc
     9541 ggcgcgaatc gctttttgca acaaacgcac tacgccaccg aactcttcat cgctctggtt
     9601 acattcacaa cgcatatgcg gcacggaaac caccggcagc ggtggcgcgg tttcggtcag
     9661 ttgctggcga agatcgttca ggcgagcagt gagacgttgt ttttcttctt cattcggagc
     9721 aaacaggctg gcctgaatgc gggtgctttt tttctgatgg tcaatcacca tcagcgtttc
     9781 agcgagataa aaacagaaat caggacagtt attttccgct gacaattgcg gtaaatcttc
     9841 aaaccccgcc acaaggtcat aagagaacag gccgccgaag aacattgctt ctcgttcttc
     9901 cttcggtaca ttcagcatat tctgcaataa gcggaaagcg tcaaaaaccg aaagggagca
     9961 taagcgagcg tcttcatcca gcagtggact gacagggggg aagcgcagca cacggcagtt
    10021 tggtaattgt tcattttcca tacccgcagg cagggcgtta tccagtagtg tcagcagggc
    10081 ttcgccgttg ccggaaagtg cctggattgt gacagtgtca cctaaagctg taatgcgcag
    10141 cgcactgtct accagcagca ggctttttaa atcatctttg ctgtcgatat ctgcggattc
    10201 cagcagcagc gttgccggac gatccccaca caactggtga aaaagcgcgg tgggattgtc
    10261 gcgataagcg ccttcgcagg ttagcagttc gagagtcggt ttttgtgttt gcattgttat
    10321 tctctaattt tgttcaaaaa aaagcccgct cattaggcgg gctgggtatc tgattgcttt
    10381 acgcatggtg aatacactgc ccgtttcagg aagtgcgcca ccaacctttc agtacgaaaa
    10441 ttgctttcat tgtcgatacc ctttttacgt gaactttcgt actagttaac tagttcgatg
    10501 attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg tcggcgcaaa
    10561 aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt gatttacgac
    10621 ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt ggtgcaccgt
    10681 gcagtcgaga tgcgcgtcgg caccctggcg atcaccgacc atgacaccac agctgcaatc
    10741 gcgccagcaa gagaggaaat ttcacgttcg ggactggcgt tgaatcttat tcccggcgtg
    10801 gaaatttcca cggtctggga aaatcatgaa attcatattg tcgggctgaa tattgatatt
    10861 actcatccgc taatgtgtga gttcctcgcg cagcagacag aacggcgcaa tcagcgggcg
    10921 cagctgattg ccgaaagact tgaaaaagcg caaatacctg gggcgctgga aggcgcacaa
    10981 cgactggcgc agggcggcgc agtgacgcgc ggtcattttg cgcgtttcct tgttgagtgc
    11041 ggcaaagcca gttcaatggc ggatgtcttt aaaaagtatc tggcgcgcgg gaaaaccgga
    11101 tacgttccgc cacagtggtg tacaatagaa caagctattg atgtcattca tcattctggc
    11161 ggtaaggcgg tgctagctca tcctgggcgg tacaatctta gtgctaaatg gctgaaaaga
    11221 ttggttgcgc attttgccga acaccacggt gacgcgatgg aagtcgcgca gtgccagcaa
    11281 tcgcccaatg aacgtaccca gctggcggcc cttgcgcgtc agcatcattt atgggcatca
    11341 caaggatctg attttcatca gccatgcccg tggatcgaac tgggtcgtaa actctggctg
    11401 cccgcaggcg ttgaaggcgt ctggcagcta tgggaacagc cgcagaacac cacagagagg
    11461 gaattatgag ccagtttttt tatattcatc ctgataaccc acagcaacgt ctgatcaacc
    11521 aggcggtgga gatcgtgcgt aaaggcgggg tgattgttta tccaactgat tccggctatg
    11581 cgctcggctg taaaattgaa gataaaaacg cgatggagcg tatttgtcgt attcgccagc
    11641 tgccggacgg tcacaacttt accctgatgt gtcgcgatct ttctgaactc tcgacctatt
    11701 catttgttga taacgttgcg tttcgtttaa tgaaaaacaa cacgccgggc aactatacct
    11761 tcatcttgaa ggggacgaag gaagtgccac gccgtctgtt gcaggaaaaa cgcaaaacca
    11821 tcggtatgcg tgtgccttct aacccgattg cgcaggcgtt gcttgaagca ctgggcgaac
    11881 cgatgctttc cacttcgcta atgctgccag gcagcgaatt taccgaatcc gatcccgaag
    11941 aaattaaaga ccgtctggaa aaacaggtag atttgattat tcacggcggc tatctcgggc
    12001 agaaaccgac aacggttatt gatcttaccg atgatacgcc ggtcgtggtg cgtgaaggcg
    12061 taggtgatgt gaagcctttc ttataagtgc gtaactctgt atactacgcg gccttaaaat
    12121 ggcgctatac ggcgccattg attctcctgt tcgacgcctg tgaaggcgac actaaaagga
    12181 agctctatga gcgaaaaatt acagaaagtg ctggcccgcg ctggccacgg ctcccgccgt
    12241 gaaatcgaat ctattattga agccggtcgt gtgagtgttg atggcaaaat tgctaaactc
    12301 ggcgatcgcg ttgaagttac ccctgggctg aaaattcgta tcgatggtca tctgatttcg
    12361 gtacgtgagt ccgctgaaca aatttgtcgc gttctggcct attacaaacc ggaaggtgag
    12421 ttgtgtaccc gtaacgatcc ggaagggcgt ccgacggtgt ttgaccgtct gccaaagctg
    12481 cgtggcgcac gctggattgc cgtaggtcgt ctggacgtta acacctgtgg tctgctgctg
    12541 ttcaccaccg atggtgaact ggcaaaccgt ttaatgcatc caagccgtga agttgaacgt
    12601 gaatacgccg tgcgtgtatt tggtcaggtt gacgacgcga aactgcgtga tttgagtcgt
    12661 ggcgtgcaac tggaagatgg tccggcagct tttaaaacca tcaagttcag cggcggcgaa
    12721 gggatcaacc agtggtacaa cgtgactctg accgaaggcc gtaaccgtga agttcgtcgt
    12781 ctgtgggaag cggttggtgt gcaggtgagc cgcctgatcc gtgttcgtta cggtgatatc
    12841 ccactgccaa aaggtctgcc acgcggtggc tggacggagc tggacctcgc ccagactaac
    12901 tatctgcgcg aactggtgga gctaccgcca gaaaccagct ctaaagtcgc tgtagaaaaa
    12961 gaccgtcgtc gcatgaaggc gaatcagatt cgtcgtgcgg tgaaacgtca cagtcaggtg
    13021 agcggcggtc gtcgttctgg cggacgtaat aacggttaat caaagtatgc cggacgtcat
    13081 atccggcatt tttacagatt agtaatcaat ccctatctgc gcttttacac cggcatcaaa
    13141 cgcatgtttg acggggcgta attcacttac cgtatctgcc agttcaagaa tatcccgatg
    13201 acaaccacga cccgtgataa tcaccgtctg ttgatgtgga cgttcattta acgcctgcac
    13261 cacttcttcc agtggcaaat agtcatacgc caccatatac gtcagttcat caagcaaaac
    13321 catatccagt gaggaatcag caagcatccg ctttgcatgt tgccagactt cgcggcaggc
    13381 ggcggtatca gactcgcggt tttgtgtatc ccaggtaaag cccgttgcca tcacctgaaa
    13441 ctcaacgcca tgtggctcca gcagattgcg ttcgccatta ggccaggtgc ctttaataaa
    13501 ctgcacgacg cctacttttt ttccgtgacc aactgcgcgt gttgccgtac caaatgccgc
    13561 ggtggttttg ccttttccat tgccggtaaa gacgatgata ataccgcgtt catcctgggc
    13621 ctgggccaca cgagcatcta ctttttcttt cactcgctgc tgacgctgtt ggtagcgttc
    13681 atcactcatt gggaaattcc tggtttacgg cccggttggg cgtcaaaggt catgccggtt
    13741 ttacggcggc tgtcatcgcc catcagccag aggtagagcg gcatgatatc agcgggtgtt
    13801 ttaagtttct gtggatcttc ggtcgggaag gcgctggcac gcattgcggt gcgcgtaccg
    13861 cctgggttaa tgcagttgac gcgcaggcgc tgctgatatt catcggccag tacctgcatc
    13921 atcccttcgg tggcaaattt cgacgctgca tatgcacccc agttagctcg tccctgacgt
    13981 ccaacgcttg atgaagtaaa gaccagtgaa ccggcgtccg atttgagtaa taaaggaagc
    14041 agcgcctggg tgagcaaaaa ggtggcatta acgttgacct gcatgacgtc ctgccagacc
    14101 tgcggatttt gttcgctcat tgggcaaaca tcgccgagca atccggcatt atgcaaaaca
    14161 ccatccagac gcggataatt aacggtaatg cgctgtgcca gttgttggca atcttcggac
    14221 gtgcaggtca gcaaatcgag aataaaccac tgtggctgac gtccagtttc ttcgtttatg
    14281 tggctggcta cctgacgtaa tttttcttca ttacggccca acagaatcac tgtcgcacca
    14341 tagcgtgcat acgtcatcgc ggcttcacgg ccaatcccat cgctggctcc cgtcaccagg
    14401 ataatgcgat cattgagtaa atcttgtttt ggctggtaat gcatggctac tcctcaacga
    14461 cgttgtctgt cgcgctgcca gtgtacttta tgactttggg gctttatgcc tgaaacagaa
    14521 gggcatttca atcagcctgg ggcgataaca ccgcaaaatg gatactttgt catactttcg
    14581 ctgcaataac atctctgcga gacggcttaa catgcctgtt gtaaactgtg agccaaagcg
    14641 ttgtttaacc aaggtgggga ctcgtggaat tgttgtctga atatggtttg tttttggcga
    14701 aaatcgttac cgttgtgcta gcgattgcgg cgattgccgc cattattgtc aatgttgctc
    14761 aacgtaataa acgccagcgt ggcgagttac gggtcaacaa tctcagcgaa cagtataagg
    14821 agatgaaaga agaactggcc gcggcgctga tggactcaca tcagcaaaaa cagtggcaca
    14881 aagcgcagaa gaaaaagcac aagcaagaag cgaaagcagc aaaagcgaaa gccaaactgg
    14941 gcgaggtggc aactgacagt aaacctcgcg tctgggtgct ggattttaaa ggcagcatgg
    15001 acgcccatga agtgaactcg ctacgtgaag agataacggc ggtactcgca gcattcaaac
    15061 cgcaggatca ggttgtgctc cgtctggaaa gccctggtgg catggtgcat ggttacgggt
    15121 tggcggcttc gcagctgcag cgtctgcgtg ataaaaacat tcctttaact gttacggtag
    15181 acaaagtcgc tgccagcggc ggttacatga tggcctgtgt ggcggacaaa attgtttccg
    15241 caccgtttgc tattgtgggt tccattgggg tggtggcgca aatgcccaac tttaaccgct
    15301 tcctgaaaag caaagatatt gatatcgaac tgcacactgc cgggcagtat aagcgtactc
    15361 tgaccttgct gggtgaaaat accgaagaag ggcgggagaa attccgcgaa gagttgaacg
    15421 aaacgcatca gttgtttaaa gattttgtga agcgtatgcg tccgtctctg gatattgaac
    15481 aggtggcaac gggtgaacac tggtacggac aacaggcggt agagaaaggc ctggttgatg
    15541 aaatcaacac cagtgatgaa gttattctta gcctgatgga aggccgtgaa gtggttaatg
    15601 tacgctatat gcagcgtaaa cgactcattg accgattcac cggcagcgcg gcagagagcg
    15661 ccgatcgatt gttgctacgc tggtggcagc ggggtcaaaa gccattgatg taaaagacaa
    15721 acgcgaggct aagacctcgc gttttgcttt aatcaaccag atgatatttt tctgaaagca
    15781 catgggccaa gtgtttgaac atattaaaca ccgcggtgct tttggctgtt ggcaatcctt
    15841 gttcatctaa aaagtagtcg ccggtaaata ccagcacgcc attacgctgc gtgacgccgg
    15901 tggcttcaat ccctgcgagc gtatcctcat gctcacgaat gattttgttg gcctctttca
    15961 acagcgtttc gcggtcgatg ggttgtgtct ctttgttcat tatttactcc ttaaacaagg
    16021 acattagtct acgccaggca tggcttgcag acaaatatac cacgctggtg gcaagagcgc
    16081 cttactggta actttggatt ttgcatgcta ataaagttgc gtatcggatt ttatcaggta
    16141 cagtgtgacg ctttcgtcaa tctggcaata gatttgcttg acattcgacc aaaattccgt
    16201 cgtgctatag cgcctgtagg ccaagacctg ttaactcagt cacctgaatt ttcgtgaaca
    16261 gagtcacgac aaggggttga tatccgcaga gaacgagtcc atatcggtaa ctcgttgcca
    16321 gtggaaggtt tatcaacgtg cgacgcattc ctggaagaat caaattaggt aaggtgaata
    16381 tgggtaaagc tcttgtcatc gttgagtccc cggcaaaagc caaaacgatc aacaagtatc
    16441 tgggtagtga ctacgtggtg aaatccagcg tcggtcacat ccgcgatttg ccgaccagtg
    16501 gctcagctgc caaaaagagt gccgactcta cctccaccaa gacggctaaa aagcctaaaa
    16561 aggatgaacg tggcgctctc gtcaaccgta tgggggttga cccgtggcac aattgggagg
    16621 cgcactatga agtgttgcct ggtaaagaga aggtcgtctc tgaactgaaa caactggctg
    16681 aaaaagccga ccacatctat ctcgcaaccg accttgaccg cgaaggggaa gccattgcat
    16741 ggcacctgcg ggaagtgatt gggggtgatg atgcgcgcta tagccgagtg gtgtttaacg
    16801 aaattactaa aaacgcgatc cgccaggcat ttaataaacc gggtgagctg aatattgatc
    16861 gtgttaatgc ccagcaggcg cgtcgcttta tggaccgcgt ggtggggtat atggtttcgc
    16921 cgctgctatg gaaaaagatc gctcgtggtc tgtctgccgg tcgtgtgcag tcggtggcgg
    16981 ttcgcctggt ggtcgagcgt gagcgtgaaa ttaaagcgtt cgtgccggaa gagttctggg
    17041 aagtcgatgc cagcacgacc acgccatctg gtgaagcgtt ggcgttacag gtgactcatc
    17101 agaacgacaa accgttccgt ccggtcaaca aagaacaaac tcaggctgcg gtaagtctgc
    17161 tggaaaaagc gcgctacagc gtgctggaac gtgaagacaa accgacaacc agtaaacctg
    17221 gcgctccttt tattacctct acgctgcaac aagctgccag cacccgtctt ggatttggcg
    17281 tgaaaaaaac catgatgatg gcgcagcgtt tgtatgaagc aggctatatc acttacatgc
    17341 gtaccgactc cactaacctg agtcaggacg cggtaaatat ggttcgcggt tatatcagcg
    17401 ataattttgg taagaaatat ctgccggaaa gtccgaatca gtacgccagc aaagaaaact
    17461 cacaggaagc gcacgaagcg attcgtcctt ctgacgtcaa tgtgatggcg gaatcgctga
    17521 aggatatgga agcagatgcg cagaaactgt accagttaat ctggcgtcag ttcgtagcct
    17581 gccagatgac cccagcgaaa tatgactcca cgacgctgac cgttggtgcg ggcgatttcc
    17641 gcctgaaagc acgcggtcgt attttgcgtt ttgatggctg gacaaaagtg atgcctgcgt
    17701 tgcgtaaagg cgatgaagat cgcatcttac cagcagttaa taaaggcgat gctctgacgc
    17761 tcgttgaact tacaccagcc cagcacttta ccaagccgcc agcccgtttc agtgaagcat
    17821 cgctggttaa agagctggaa aaacgtggta tcggtcgtcc gtctacctat gcgtcgatca
    17881 tttcgaccat tcaggatcgt ggctacgtgc gagtagaaaa tcgtcgtttc tatgcggaaa
    17941 aaatgggcga aatcgtcacc gatcgccttg aagaaaattt ccgcgagtta atgaactacg
    18001 actttaccgc gcagatggaa aacagcctcg accaggtggc aaatcacgaa gcagagtgga
    18061 aagctgtact ggatcacttc ttctcggatt tcacccagca gttagataaa gctgaaaaag
    18121 atccggaaga gggtggtatg cgcccgaacc agatggttct gaccagcatt gactgcccga
    18181 cttgtggtcg caaaatgggg attcgcacag cgagcaccgg ggtattcctt ggctgttctg
    18241 gctatgcgct gccgccgaaa gagcgttgca aaaccaccat taacctggtg ccggaaaacg
    18301 aagtgctgaa cgtgctggaa ggcgaagatg ctgaaaccaa cgcgctgcgc gcaaaacgtc
    18361 gttgcccgaa atgcggcacg gcgatggaca gctatctcat cgatccgaaa cgtaagttgc
    18421 atgtctgtgg taataaccca acctgcgacg gttacgagat cgaagagggc gaattccgca
    18481 ttaaaggtta tgacggcccg atcgttgagt gtgaaaaatg tggctctgaa atgcacctga
    18541 aaatggggcg tttcggtaaa tacatggcct gcaccaacga agagtgtaaa aacacacgta
    18601 agattttacg taacggcgaa gtggcaccac cgaaagaaga tccggtgcca ttacctgagc
    18661 tgccgtgcga aaaatcagat gcttatttcg tgctgcgtga cggtgctgcc ggtgtgttcc
    18721 tggctgccaa cactttcccg aaatcgcgtg aaacgcgtgc gccactggtg gaagagcttt
    18781 atcgcttccg cgaccgtctg ccggaaaaac tgcgttatct ggccgatgcg ccacagcagg
    18841 atccggaagg taataagacc atggttcgct ttagccgtaa aaccaaacag caatatgtct
    18901 cttcggaaaa agacggaaag gcgactggct ggtcagcatt ttatgttgat ggcaaatggg
    18961 ttgaaggaaa aaaataacct ttaattctgt caggttttta taaacaaagg gtcgcgaaag
    19021 cggccctttt ttattgcata
//
LOCUS       DAZGTR010000085        18917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGTR010000085 DAZGTR010000000
VERSION     DAZGTR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.085"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            374..1690
                     /gene="fucP"
                     /locus_tag="ACHLCY_003300"
     CDS             374..1690
                     /gene="fucP"
                     /locus_tag="ACHLCY_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0370491.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1723..3498
                     /gene="fucI"
                     /locus_tag="ACHLCY_003301"
     CDS             1723..3498
                     /gene="fucI"
                     /locus_tag="ACHLCY_003301"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP0370492.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3607..5025
                     /gene="fucK"
                     /locus_tag="ACHLCY_003302"
     CDS             3607..5025
                     /gene="fucK"
                     /locus_tag="ACHLCY_003302"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP0370493.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            5027..5449
                     /gene="fucU"
                     /locus_tag="ACHLCY_003303"
     CDS             5027..5449
                     /gene="fucU"
                     /locus_tag="ACHLCY_003303"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP0370494.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5507..6238
                     /gene="fucR"
                     /locus_tag="ACHLCY_003304"
     CDS             5507..6238
                     /gene="fucR"
                     /locus_tag="ACHLCY_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP0370495.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6282..7382)
                     /gene="rlmM"
                     /locus_tag="ACHLCY_003305"
     CDS             complement(6282..7382)
                     /gene="rlmM"
                     /locus_tag="ACHLCY_003305"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP0370496.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWLVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7375..7770)
                     /gene="ygdD"
                     /locus_tag="ACHLCY_003306"
     CDS             complement(7375..7770)
                     /gene="ygdD"
                     /locus_tag="ACHLCY_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP0370497.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7789..8706)
                     /gene="gcvA"
                     /locus_tag="ACHLCY_003307"
     CDS             complement(7789..8706)
                     /gene="gcvA"
                     /locus_tag="ACHLCY_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP0370498.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(9057..9284)
                     /gene="ygdI"
                     /locus_tag="ACHLCY_003308"
     CDS             complement(9057..9284)
                     /gene="ygdI"
                     /locus_tag="ACHLCY_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP0370499.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9476..10681
                     /gene="csdA"
                     /locus_tag="ACHLCY_003309"
     CDS             9476..10681
                     /gene="csdA"
                     /locus_tag="ACHLCY_003309"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP0370500.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQLGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLNIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10681..11124
                     /gene="csdE"
                     /locus_tag="ACHLCY_003310"
     CDS             10681..11124
                     /gene="csdE"
                     /locus_tag="ACHLCY_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP0370501.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11175..11981)
                     /gene="tcdA"
                     /locus_tag="ACHLCY_003311"
     CDS             complement(11175..11981)
                     /gene="tcdA"
                     /locus_tag="ACHLCY_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP0370502.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12058..13155)
                     /gene="mltA"
                     /locus_tag="ACHLCY_003312"
     CDS             complement(12058..13155)
                     /gene="mltA"
                     /locus_tag="ACHLCY_003312"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP0370503.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13364..13440
                     /locus_tag="ACHLCY_003313"
     tRNA            13364..13440
                     /locus_tag="ACHLCY_003313"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13398..13400,aa:Met,seq:cat)
     gene            complement(13520..14467)
                     /locus_tag="ACHLCY_003314"
     CDS             complement(13520..14467)
                     /locus_tag="ACHLCY_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0370504.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(14539..15135)
                     /locus_tag="ACHLCY_003315"
     CDS             complement(14539..15135)
                     /locus_tag="ACHLCY_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP0370505.1"
                     /translation="MNNTDLIHLIKRFMRNEHKVVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRAYFGLYHPGGALGIKANS"
     gene            complement(15104..16312)
                     /locus_tag="ACHLCY_003316"
     CDS             complement(15104..16312)
                     /locus_tag="ACHLCY_003316"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP0370506.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGEGYLRLNVACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            complement(16312..17878)
                     /locus_tag="ACHLCY_003317"
                     /pseudo
     CDS             complement(16312..17878)
                     /locus_tag="ACHLCY_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(17910..18734)
                     /locus_tag="ACHLCY_003318"
     CDS             complement(17910..18734)
                     /locus_tag="ACHLCY_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0370507.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDSLLEEKLYLSIRIKVLMD"
BASE COUNT         5020 a         4762 c         4297 g         4838 t
ORIGIN      
        1 tataacccca ttaaaaatga ccgctcttaa aaatatttat caaaacggtc atttttctat
       61 tcactcaagc caggaatgcc cgttttcggc ataaacaatt aatacggtca tctggtttgt
      121 gttttttatg atttattttc tgaaacgggc atgaaatttc gattattaaa gtgatggtag
      181 tcacataaag tcaccttcca gctaataagt gtgaccgccg tcatattaca gagcgttttt
      241 tatttgaaaa tgaatccatg agttcatttc agacaggcaa atattcactg atatgaagcc
      301 cgaactcgct ggttttgcac ttttgaaaac ataaccgatt acgtgcttaa gcttctgaac
      361 ctaagaggat gctatgggaa acacatcaat acaaacgcag agttaccgtg cggtagataa
      421 agatgcaggg caaagcagaa gttacattat tccattcgcg ctgctgtgct cactgttttt
      481 tctttgggcg gtagccaata accttaacga cattttatta cctcaattcc agcaggcttt
      541 tacgctgaca aatttccagg ctggcctgat ccaatcggcc ttttactttg gttatttcat
      601 tatcccaatc cctgctggga tattgatgaa aaaactcagt tataaagcag ggattattac
      661 cggattattt ttatatgcct tgggcgctgc attattctgg cccgctgcag aaataatgaa
      721 ctacacctta tttttagttg gcctatttat tattgcagcc ggattaggtt gtctggaaac
      781 tgccgcaaac ccttttgtta cggtattagg gccggaaagc agtggtcact tccgcttaaa
      841 tcttgcgcaa acatttaact cgtttggcgc aattatcgcg gttgtctttg ggcaaagtct
      901 tattttgtct aacgtgccac atcaatcgca agacgttctc gataaaatgt ctccagagca
      961 attgagtgcg tataaacaca gcctggtatt atcggtacag acaccttata tgatcatcgt
     1021 ggctatcgtg ttactggtcg ccctgctgat catgctgacg aaattcccgg cattgcagag
     1081 tgataatcac agtgacgcca aacaaggatc gttctccgca tcgctttctc gcctggcgcg
     1141 tattcgccac tggcgctggg cagtattagc gcaattctgc tacgttggcg cacaaacggc
     1201 ctgctggagc tatttgattc gctacgctgt agaagaaatt ccaggtatga ctgcaggctt
     1261 tgccgctaac tatttaaccg gaaccatggt gtgcttcttt attggtcgtt tcaccggtac
     1321 ctggctcatc agtcgcttcg caccacacaa agtcctggcc gcctacgcat taatcgctat
     1381 ggcactgtgc ctgatctcag ccttcgctgg cggtcatgtg ggcttaatag ccctgacttt
     1441 atgcagcgcc tttatgtcga ttcagtaccc aacaatcttc tcgctgggca ttaagaatct
     1501 cggccaggac accaaatacg gttcgtcctt catcgttatg accatcattg gcggcggtat
     1561 tgtcactccg gtcatgggtt ttgtcagtga cgcggcgggc aacatcccca ctgctgaact
     1621 gatccccgca ctctgcttcg cggtcatctt tatctttgcc cgtttccgct ctcaaacggc
     1681 aactaactga acatattttc cgaataaagt gaggaatctg taatgaaaaa aatcagctta
     1741 ccgaaaattg gtatccgccc ggttattgac ggtcgtcgca tgggtgttcg tgagtcgctt
     1801 gaagaacaaa caatgaatat ggcgaaagct acggccgcac tgctgaccga gaaactgcgc
     1861 catgcctgcg gagctgccgt cgagtgtgtc atttccgata cctgtatcgc gggtatggct
     1921 gaagccgctg cttgcgaaga aaaattcagc agtcagaatg taggcctcac cattacggta
     1981 acgccttgct ggtgctatgg cagtgaaacc atcgacatgg atccaacccg cccgaaagcc
     2041 atttggggct ttaacggcac tgaacgtccc ggcgctgttt acctggcagc ggctctggca
     2101 gctcacagcc agaaaggcat cccggcattc tccatttacg gtcatgacgt tcaggatgcc
     2161 gatgacacat cgattcctgc cgatgttgaa gaaaaactgc tgcgctttgc ccgcgccggt
     2221 ttggccgtcg ccagcatgaa aggtaaaagc tatctgtcgc tgggcggcgt ttcgatgggt
     2281 atcgccggtt ccattgttga tcacaacttc tttgaatcct ggctgggaat gaaagtccag
     2341 gcggtggata tgaccgaact gcgtcgccgt atcgatcaga agatttacga cgaagccgaa
     2401 ctggaaatgg cactggcctg ggctgataaa aacttccgct atggcgaaga tgaaaataac
     2461 aaacagtatc aacgcaatgc cgagcaaagc cgcgcagttc tgcgcgaaag tttactgatg
     2521 gcgatgtgta tccgcgacat gatgcaaggc aacagcaaac tggccgatat tggtcgcgtg
     2581 gaagaatcac ttggctacaa cgccatcgct gcgggcttcc aggggcaacg tcactggacc
     2641 gatcaatatc ccaatggtga caccgccgaa gcgatcctta acagttcatt tgactggaat
     2701 ggcgtgcgcg aaccctttgt cgtggcgacc gaaaacgaca gtcttaacgg cgtggcaatg
     2761 ctaatgggtc accagctcac cggcaccgct caggtatttg ccgatgtgcg tacctactgg
     2821 tcaccagaag caattgagcg tgtaacgggg cataaactcg atggactggc agaacacggc
     2881 atcatccatt tgatcaactc cggttctgct gcgctggacg gttcctgtaa acaacgcgac
     2941 agcgaaggta acccgacgat gaagccgcac tgggaaatct ctcagcaaga ggctgacgct
     3001 tgcctcgccg ctaccgaatg gtgcccggcg atccacgaat acttccgtgg cggcggttac
     3061 tcttcccgct tccttaccga aggcggcgtc ccgttcacca tgacccgtgt caacatcatc
     3121 aaaggcctgg gaccggtact gcaaatcgcg gaaggctgga gcgtggaatt gccgaaggat
     3181 gtgcatgaca tcctcaacaa acgcaccaac tcaacctggc caaccacctg gtttgcaccg
     3241 cgcctcaccg gtaaagggcc gtttacggat gtgtactcgg taatggcgaa ctggggcgct
     3301 aaccatgggg ttctgaccat cggccacgtt ggcgcagact ttatcactct cgcctccatg
     3361 ctgcgtatcc cggtatgtat gcacaacgtt gaagagacca aagtgtatcg tccttctgcc
     3421 tgggctgcgc acggcatgga tattgaaggc caggattacc gcgcttgcca gaactacggc
     3481 ccgttgtaca agcgttaata cattactccc tgatgtgatg cccggtcgct ccggctaccg
     3541 ggcctgaaca agcaagagtg gttagccgga taagcaatgt tatccggcta tattgcagga
     3601 gcgattatga aacaagaagt tatcctggta ctcgactgtg gcgcgaccaa tgtcagggcc
     3661 atcgcggtta atcggcaggg aaaaattgtt gcccgcgcct caacgcctaa tgccagcgat
     3721 atcgcgatgg aaaacaacac ctggcaccag tggtctttag acgccatttt gcaacgcttt
     3781 gctgattgct gtcggcaaat caatagtgaa ctgactgaat gccacatccg cggtatcgcc
     3841 gtcaccacct ttggtgtgga tggcgctctg gtagataagc aaggcaatct gctctatccg
     3901 atcattagct ggaaatgtcc gcgaacagca gcggttatgg acaatattga acagttgatc
     3961 tccgcacagc ggttgcaggc tatttctggc gtcggagcct ttagtttcaa tacgttatat
     4021 aagttggtgt ggttgaaaga aaatcatcca caactgctgg aacgcgcgca cgcctggctc
     4081 tttatttcgt cgctgattaa ccaccgttta accggcgaat tcactactga tatcacgatg
     4141 gccggaacca gccagatgct ggatatccag caacgcgatt tcagtccgca aattttacaa
     4201 gccaccggta ttccacgccg actcttccct cgtctggtgg aagcgggtga acagattggt
     4261 acgctacaga acagcgccgc agcaatgctc ggcttacccg ttggcatacc ggtgatttcc
     4321 gcaggtcacg atacccagtt cgcccttttt ggcgctggtg ccgaccaaaa tgaacccgtg
     4381 ctctcttccg gtacatggga aattttaatg gtccgtagcg cccaggttga tacttcgctg
     4441 ttaagtcagt acgccggttc cacctgcgaa ctggatagcc aggcagggtt gtataaccca
     4501 ggtatgcaat ggctggcatc cggcgtgctg gaatgggtga gaaaactgtt ctggacggct
     4561 gaaacaccct ggcaaatgtt gattgaagaa gctcgtctga tcgcgcctgg cgcggatggc
     4621 gtaaaaatgc agtgtgattt attgtcgtgt cagaacgctg gctggcaagg agtgacgctt
     4681 aataccacgc gggggcattt ctatcgcgcg gcgctggaag ggttaactgc gcaattacag
     4741 cgcaatctac agatgctgga aaaaatcggg cactttaagg cctctgaatt attgttagtc
     4801 ggtggaggaa gtcgcaacac attgtggaat cagattaaag ccaatatgct tgatattccg
     4861 gtaaaagttc tcgacgatgc cgaaacgacc gtcgcaggag ctgcgctgtt cggttggtat
     4921 ggcgtagggg aattcaacag cccggaagaa gcccgtgcac agattcatta tcagtaccgt
     4981 tatttctacc cgcaaactga acctgaattt atagaggaag tgtgaaatgc tgaaaacaat
     5041 ttcgccgtta atttctcccg aactattgaa agtgctggca gagatgggac atggagatga
     5101 aattattttt tccgatgctc actttcccgc ccattcgatg ggaccgcagg tgatccgcgc
     5161 tgatggcctg ttggtgagcg acttgctcca ggcgattatc ccgttatttg agctggacag
     5221 ttatgccccg ccgctggtga tgatggcagc ggtagaaggt gacactctcg atcctgaagt
     5281 agaacgacgt taccgtaatg cgctttcact acaagccccg tgtcctgaca tcatccgcat
     5341 caatcgtttt gcgttttatg aacgggcgca aaaagccttt gcgatcgtta tcacaggcga
     5401 acgagcgaag tacgggaata ttcttttaaa aaaaggggta acaccgtaat ctcataccgg
     5461 tacgcccgca tgacgcgggc ggttatcgaa tgatggggtg aaaaatatga aagcggcacg
     5521 ccagcaagcg atagtcgacc tgctgctgaa ccataccagc ctgaccacgg aagctctctc
     5581 tgaacagcta aaggtcagta aagaaaccat tcgtcgcgat ctcaatgaat tacagacgca
     5641 gggtaaaatt ctgcgcaatc atggacgcgc taaatatatc caccgtcaaa atcaagacag
     5701 tggcgatccc tttcacatcc ggctgaaaag ccattatgcg cataaagcag atatcgcgcg
     5761 cgaggcgctc gcgtggattg aagaagggat ggtgatagcc ttagacgcca gttcaacttg
     5821 ctggtatctg gcacgccagt tgcctgacat caacattcag gtcttcacca atagccatcc
     5881 gatttgccat gaactcggta aacgcgaacg cattcaactg atcagttccg gcggcacact
     5941 tgagcgcaaa tatggctgtt acgtcaatcc ctcgctgatt tcccaactta aatcgctgga
     6001 aatcgatctg tttatttttt cttgtgaagg gatcgatagc agcggcgcac tgtgggactc
     6061 caatgccatc aacgctgatt acaaatcgat gctattaaaa cgtgccgcgc aatcgttgtt
     6121 attgattgat aaaagtaaat ttaatcgttc aggggaagcc cgcatcgggc atctggatga
     6181 ggtaacgcac attatttctg atgagcgcca ggttgcaact tctttggtaa cagcctgacg
     6241 gaaagggtag caggccggag acgacgcccc ggccttgcct gttatcgctc gtcgcgacga
     6301 ccacccaccg cagcccagat gcggcggacg tgcaccgtca cttcttcgcg atcgtgatac
     6361 aactgccgcg cctgaatctg agcatttatg ccatgttcat caagctgtgc ctgaatatac
     6421 gccagattgt gtgatacttc ttcgtagcgt tttttcatcg gcagtttgag attgaaaatg
     6481 gtttcacggc accagccatt aaccagccac tgcgccatca atgccgcaac tttagccggt
     6541 ttttcaacca tatcgcatac caaccaggag atattgctgc gtgtcggacg gaatttgaaa
     6601 ccgtcttccc gcagccacgt cacctgcccg gtatccatca gactttgcgc catcgggccg
     6661 ttgtcgacgg aataaaccca catgttgcgc ttcaccagtt ggtaggtcca gccgcctggg
     6721 caagcgccca aatccaccgc ccacatcccg ttcgccaggc gttcatccca ctcatccgca
     6781 ggaataaaca catgaaatgc ctcttccagt ttgagtgtgg aacgactcgg cgcatctgcc
     6841 ggaaatttca ggcgcggaat gcccatatag aacggcgaat tattgttgct gtatgagtaa
     6901 ccggtatagc agcaacctgg tgcaatgaag aatacatgca caaccggacg cttcggcgtt
     6961 tcatagttcg ccagcacccc ggcatcgcgc aaggcagcgc gtagcggcac ggtgaattta
     7021 cggcagaatt tcagcaactc tttgctttcg ttggtatcgg caacttcaac acgcagttca
     7081 ccgcccttct ctactacgcc ctgtaacatg ccgacaatgg gggtaatacg atcttctggc
     7141 ggcaaatgct gcaggagttc ccccaccaca aaccactggc gggcaaaaat taatgaactg
     7201 aacggcagct cacggattaa cttatcacca tcatcaggtt gataacattc ataaatgaca
     7261 taacccgcat tctctttcac gcgggcaaaa ccgaaaattt cccgctggcc ggctttatcg
     7321 gtaatttctg cggcgcactc tttttcaaag cccggacggc acagcaatac aaccttattc
     7381 atgacttacg cccttgcgct ttaaacggat agcaccaact aacattaacg cccagcccgc
     7441 aaggaagctc acgccgccaa ccggagtgac aaacgcccac aaacgcagat gggacagtgc
     7501 caggcaataa aggctgccgc tgaacagcac cgtgcctaac gcgaggaaaa cgctactcca
     7561 gtaaaaccag atactgatgc gacgctgcat tgccaccgcc agacctaaga tcgccagcgt
     7621 atgaaacgcc tggtattcga ggccggtctg gatccagccc atctcaacgg cccccatggt
     7681 tttacttaac acatgcgcgc caaaagcgcc cagagccaca aaaatgaagc cgctaatggc
     7741 ggcgaaaatc agcataaaac ggctggtcat ggtcgtaccc tacgtaaatt attgttcata
     7801 acgaaagcgg aatttttctt gttcagcagc ggctttcgcc aggatccatt ggcgaaaggc
     7861 ggctatttta cccagttctg cctggctgtc atgacaaacc agataaaaag cgtttttact
     7921 gaccagaaca tcattaaacg ggcaaacaag acgtccggct tcaatttcag attgcgccat
     7981 cacgttattt gccagcgcca ctccctgccc gtgaatagcc gcttgcagca ccatggcgct
     8041 atgactaaaa attggccctt gctgaacgtt gatatgattt aaccccaact gtcgggtata
     8101 tgtctgccag tcacggcgtg acgcatcatg taataacgta tgtttagcca gatcttccgg
     8161 ggtcttcaag ggtttttcgc ctgtcagcag tagcggcgaa cacaccggca ataaatattc
     8221 ggcgtacagt ttttccaccc gtagccccgg ccagttgccc cgaccataaa atatcgccac
     8281 atcaacatca tccgccagct tatcttcctg acgatcaacc gcctggattc gaacgtcaat
     8341 tcccggataa gctgaattaa agctggaaag tcgcggaacc aaccaatgaa tggcgaaact
     8401 ggggagtaaa ctgaccgtca acgccccctt ggcgctacgg gcctggagtt tacgcgtcgc
     8461 ttcggttaat tgcgaaaata tctctttgat atcgaggaaa tagctttgcc cttcctcggt
     8521 cagcaggagt gaacgattac ggcggcggaa cagttttagc cccaaaaaat cctcaagaga
     8581 cttgatttga tgacttactg cggcctgggt cacaaaaagc tcttctgctg cgcgagtgaa
     8641 acttaaatgg cgtgctgcgg catcaaaaac tcgtaaggca tttagcggtg gtaatcgttt
     8701 agacatggct tttaaacttt gatgttaaat gaatttaaca attagatcac actgtgtaac
     8761 ctattagttt ttttaatctg agccattata aattgtccgt tgagcttcta ccagcaaata
     8821 cctatagtgg cggcacttcc tgagccggaa cgaaaagttt tatcggaatg cgtgttctgg
     8881 tgaacttttg gcttacggtt gtgatgttgt gttgttgtgt ttgcaattgg tctgcgattc
     8941 agaccatggt agcaaagcta ccttttttca cttcctgtac atttaccctg tctgtccata
     9001 gtgattaatg tagcaccgcc taattgcggt gctttttttt accttgcgat cgtgaattac
     9061 tgatccagtt cgaccatctc tttcacgtca gtacggttga tctgctgttt gttgccatta
     9121 gcgtctttat acgaaatcat accggtatcg ttatcagtct gtggtttgcc gtcagagacg
     9181 atggtacgtc cgtcattggt gtgcatcaca tagttcgaac cggaacaggc gctcagggca
     9241 aaagtcagca tacaggcaga aataattgcg gcagtctttt tcatcatctt ctccttaaag
     9301 cgattaataa atcttaatag tattgattaa caggctaaaa ttaacgccta acactattca
     9361 gcatatgtta cttacgcggc ttcgccagga tatccagata attctgatgg ttagcactct
     9421 ccttgtatca aagtgaattt tgcgtcacga tcggtgcatc aagccgagga gtaccatgaa
     9481 cgtttttaat cccgcgcagt ttcgcgccca gtttcccgct ctacaggatg cgggcgtcta
     9541 tctcgacagc gccgcgaccg cgcttaaacc tgaagccgtg gttgaagcca cccgacagtt
     9601 ttacagcctg agcgccggaa acgtccatcg cagccagttt gccgaagccc aacgcctgac
     9661 cgcgcgttat gaagctgcac gagagaaagt ggcgcaatta ctgaatgcac cggatgataa
     9721 aactatcgtc tggacgcgcg gcaccactga atccatcaac atggttgcac aatgctatgc
     9781 gcgtccgcgt ctgcaactgg gcgatgagat tattgtcagc gtggcagaac accacgccaa
     9841 cctcgtcccc tggctgatgg tcgcccaaca aactggagcc aaagtggtga aattgccgct
     9901 taatgcgcag cgactgccgg atgtcgattt gttgccagaa ctgattactc cccgtagtcg
     9961 gattctggcg ttgggtcaga tgtcgaacgt tactggcggt tgcccggatc tggcgcgagc
    10021 gattaccttt gctcattcag ccgggatggt ggtgatggtt gatggtgctc agggggcggt
    10081 gcatttcccc gcggatgttc agcaactgaa tattgatttc tatgcttttt caggtcacaa
    10141 actgtatggc ccgacaggta tcggcgtgct gtatggcaaa tcagaactgc tggaagcgat
    10201 gtcgccctgg ctgggcggcg gcaaaatggt tcacgaagtg agttttgacg gcttcacgac
    10261 tcaatctgcg ccgtggaaac tggaagcagg aacgccaaat gtggctggtg tcataggatt
    10321 aagcgcggcg ctggaatggc tggcagatta cgatatcaac caggccgaaa actggagccg
    10381 tagcttagca acactggcgg aagatgcgct ggcgaaacgt ccaggtttcc gttcattccg
    10441 ctgccaggat tccagcctgc tggcctttga ttttgctggc gttcatcaca gcgatatggt
    10501 gacgctgctg gcggagtacg gtattgcctt gcgggccggg caacattgcg ctcagccgct
    10561 actggcagaa ttaggcgtaa ccggcacact gcgcgcctct tttgcgccat ataatacaaa
    10621 gagtgatgtg gatgcgctgg tgaatgccgt tgaccgcgcg ctggaattat tggtggatta
    10681 atgacgaacc cgcaattcgc cggacatccg ttcggcacaa ccgtaaccgc agaaacgtta
    10741 cgcaatacct tcgcaccgtt gacgcaatgg gaagataaat atcgccagtt gatcatgctg
    10801 gggaaacagc ttccggcttt gccagacgag ttaaaagcgc aggctaaaga gattgccgga
    10861 tgcgaaaacc gcgtctggct gggatatacc gtggctgaaa acggtaaaat gcacttcttt
    10921 ggcgacagcg aaggacgtat agtgcgcggc ctgctggcgg tgctgttgac tgccgttgag
    10981 gggaaaaccg ccgccgaatt gcaggcacag tcgccgctgg cattgtttga tgagctggga
    11041 ttgcgtgggc agcttagcgc ctcacgcagc caggggttga atgcgttaag cgaggcgatt
    11101 atcgctgcga cgaagcaggt ttaagcacag aaccaggtcg gataagacgt tcgcgtcgca
    11161 tccgacctga ttgtttaacc ctgacgcgcc gctttcgcca tcatcttctt cagcgcatga
    11221 gaaaccgcaa caaacccaaa ggtggcggtc accatcgttg ccgcgccaaa tccagatgca
    11281 caatccatcc gcttcggccc ttctgccgtg gctttcatcg cacacacagt accgtctgac
    11341 tgcgggtaca ccagcgcttc agtagaaaac acgcaatcga cgccgagctt acctttactg
    11401 tttttcacta cgccaaaatc gcttttcagg cgctcgcgca acttcgccgc cagcggatcc
    11461 tgaatcgttt tcgccagatc ggtaacctga atctgcgtcg ggtcaatctg cccacccgcg
    11521 ccaccggtcg tgaccagcgg gattttattg cgccgacaat aagcaatcag cgccgctttg
    11581 ggccgtacac tatcaatggc atcaatcacg tacgaatacc ccacgctcat atactgcgct
    11641 acgttatccg gcgtcacgaa atcatccacc accgttacac ggcactccgg gttaatctgg
    11701 cgaatacgct ccgccatgac ttccgctttt gccagcccga cgttatcgcg cagagcgtga
    11761 atttgccgat tggtattggt gacgcacaca tcatccatat cgataagcgt gattgcgcca
    11821 atccccgtgc gcgccagcgc ttccgccgcc caggaaccga caccaccgat accaaccaca
    11881 caaatatgcg cgtcagcaaa cagttgcaac gctttttcac catacagacg cgctgtgcca
    11941 ccaaaacgct gacgccatgc atcactaatt accacagaca tagcacctca gaattaaaaa
    12001 gggtgaggtt atccctcacc ctgtcgtatc cgtaaaaacg gtatacagaa taacacatca
    12061 gccgctaaag acgttacctg cgcccggggc ggttttcagc acccagacac gtccatagtg
    12121 gttgtaccaa cctgcgcgat gtccggcttc cggcccgatc ccttgataga tatcgaagtg
    12181 ttggcctttg attgcgccac cgacatccag cgccaccatc agacgcaatt cgtactgacc
    12241 gttaaatttg ccgttattat ccagcagcgg cacttctgcc agcaaggtag tacctggcgg
    12301 aataattgaa cgatcggagg caacagaagc acggccaacc agcggcaccg cacttgcccc
    12361 tttcaccggt gcaaaagatt gcggtttaaa gaaaacgaaa gacgggttct gttccagcag
    12421 ctcgcgaacc tcggcttcac tgtgtgtttc gccccaatga cgaatcgcct gcatcgacat
    12481 atcttctttt ttcacttcgc cacggtcgat cagcacctta ccaatgctgc gataggcatg
    12541 accgtttttc cctgcatagc tgaaaaagtt aagcggactg ccatcaccaa agtcgatata
    12601 cccgctaccc tgcacatcca taatgaagtt atccatcagg gagttgctgt aagcgagaat
    12661 atatttatca ctcagcgccc ctgcgtagat ctccgcacga gacggcagac gaccacgttt
    12721 tggcggcata cggtaaatag gatactggaa ctcgccctgg cgggtatggc gcgcctgaat
    12781 taccggcgtg taatagcctg taaactgcac gttaccatag ttgtcggcac cttccatctg
    12841 ccaggcatca atgccgaact ggcgcatatt gcgggtatca ccgcctgcgc gcagccactc
    12901 ttgcaccgcg ttataaacat tactctggtt gccatacaga cgcggtgacg aattacggat
    12961 atggttaatt tgctcggcaa aatcaccggc gttaatcggc gcgccaacgg catctggctg
    13021 gttcaccaga gagaaaggct gggtaaattt cccgtcttta tattgctgtc cgcgatcggt
    13081 tggtttggaa gagcaggcgg caagcattgc cacaaccgtg cccataagaa ggtactttac
    13141 ccaacgtcct ttcattgttc tcttcttcag gttaaaaaat aaggcgcaac gaagataaca
    13201 aaccgccgcg tttaatgaaa tgagcagcct ctccctgacg caaattttgc acaaaaaata
    13261 ggctttagtg gtttgttttt gttcaaaatc atgctaaatc cgtgatcggg gtaaaaaaaa
    13321 ggttgcatga aaacgcgagc ggagtatagt gcgcatccac ggacgcgggg tggagcagcc
    13381 tggtagctcg tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca
    13441 atacttagaa tgctgataaa ccgcaatgtg ttccacaaat tgcggttttt tttgtattta
    13501 aaaacttcgt tcgctgaact cacaacacgt tttttatctc ccttcctgcg aagtaatcaa
    13561 ttatcgactg ggcaacgtgt gtaccggttt gctggtatga ctcaacggtg gcggccgcaa
    13621 tatgcggcgt tgtaatgata tttttggcat gtagcagtgg gctaaattgc gccaacggtt
    13681 cagagctaaa cacatccagc gccgcaaaca catttcgttc ttcaatcacc tgtagtaaag
    13741 cgccctcatc cagcaacttt ccccttgcgg cattgatgat tagagcacca ttgcgcatca
    13801 tcgacaaata tttatgacta atgaagttct ccagctcagg agaaaaaggc aaaagaacaa
    13861 tgatgacatg cgactgcata aagatatctt caattgatac aaactgaact ccaactttgt
    13921 cagcaacctg ataatcgggt tgtttgtcat aagctaaaag ctcaacatca aaaccggata
    13981 acctttttgc cagggtttta cctatattgc cgtagccaac catgccaatc cgtttgcctt
    14041 gtaattcata gccatgcgac tcaccccagt agccattttg catcgcatga tagctgcctg
    14101 gaatatttct catgctcgcg aagataagtc caatgataaa ttccgcgaca gcatttgaat
    14161 taatcccaac cgcattattg actacaatac cggattgttg tgctgcacga agatcaatat
    14221 tatctatccc cgtcccgaat ctcgatatta ttttcacacc aggcagcaat gatagcgtgt
    14281 ccgcctgata acaatctttt ccggcgatga ccgcgcaaat agattctcgt aatgcagtgg
    14341 attctgattt aagcagatgc tccacattat tcttaataat aagattaaaa ttattctctc
    14401 ttagcaactt gctggcggaa acacagcgtg ctgaaaagct ggggacggtg accagaacat
    14461 ttttcatagc tgacactcac ttcaatgaac gccaccccaa aagatggcgt gaccggttac
    14521 tgtatttata ctttgaaatt atgaatttgc tttaatgccg agtgcacctc ctggatgata
    14581 taagccgaaa taagcacgag taaatttttt caattcagag agtgcaagcg ccaccgcatc
    14641 accaacaacc agaacaacag tagacgagca tgacggtgct aaacctaaat tatccgcctc
    14701 acttttgacg gggatttcaa cagataaatc acaacttatc gcaagcgatg agtgatgact
    14761 gcgggtaaaa gagatcaaat aattacccat ttttttcaaa ctaggcagtg tcgccaggat
    14821 ttccgccgtc tcaccactgt ttgaaataag aataacaaca tcgtctttcg ccaccatccc
    14881 aaggtcgccg tgtaccgctt ccgtaccgtg aacaaaaaac gagggagttc cggtgctggc
    14941 aaatgttgca gcaagttttc tggcaataat gccagatttc ccaacaccaa taaaaacaac
    15001 ttttccctgg caagattgta atattttaat taaattcgcg acttccgaaa ggggggaatc
    15061 aataacctct tctactactt tatgttcgtt acgcataaaa cgtttaataa gatggataag
    15121 atccgtattg ttcattttct ttttcctgat taatattttt aattacactc gcaaggcgcg
    15181 caagccccat atataatttt tctcgactgc aggccacatt aagacgcaga tagccctctc
    15241 cttcagtacc ataaacatgc cccgccataa tacctactct cccttcgtta attaatcgac
    15301 ggtaaaattt atcaaaggga taaccaaggg ccgagcaatc aacccacgaa aaatagcagc
    15361 cctcagctgg ccatacgatc aattccggaa tattttcatt taaatactgc aaggtaaatt
    15421 gaatgttaga ggcaatatgt acctttaacg actttaacca ttcttcacat tcattatagg
    15481 caacaatggt cgcaattaca ttcagtactg atggggagtt tacgaaatcg cgatacctcg
    15541 atatcgttct gaacttatga ttaaaattat cgtcagttga aatcatatat gatccggtta
    15601 gcgcaggaac attaaatgtt ttcgaaatcg aattaaacac caccactttg tcatctttta
    15661 ttgttagcgc tgacgtgaaa ctatgattaa aaacaaaatc actatgtatt tcatctgaac
    15721 ataaccaaat attatgcctt gcacagcatg ctgcgattct gtgaagatcg tcttccgacc
    15781 agaccgttcc tgtcgggtta tgaggattac ataataacac catcttcgcg cttttaattt
    15841 taagctcaaa gtcttgccag ttgataacgt agcctctccc attttctttc atcaaagagg
    15901 ttgttagcat gtttcgacca ttatgcttaa tcacatcaaa aaatgcgtta taaacaggcg
    15961 tgaaaatgag aatattatct ccattcaatg tcaatatttc tattgcctta gatattgaat
    16021 acatcacgct tgggctatag gtgatccatt ctggttgaat ctctgtttta tattgttgat
    16081 gataccaatt gactattgaa ttcagatagt catcatgctg ccaacgactg taccctaaaa
    16141 tcgggtgttc cagacgtttt tttaatgttc tgatgatagc ttccggtatc ggaagatcca
    16201 tatctgaaat agagaatggt aatacatctt ttacaccaaa tctgtcttca caaaagtccc
    16261 attgtgtgca atatgttcct ttacgagaaa ttgaggtatt aaaaatatcc atcaacatat
    16321 ccccatgtaa ttatccatat cccgacgtag ggcgatgact tttgtaccga taatgacttg
    16381 taaggtattt tgatcaagtt tcacaaccgc aattcctccg gcccttttta ttgcagcctc
    16441 atctatcaat cccatatccg cgatagttaa acgcaaacga gttacacaat tatccagtga
    16501 tttaatattt tctttaccac ccagagcctt aaccatttgc tccgctgcct gcaactctga
    16561 tttatcatga actgggatgt tagctattgc agtttcttcg tgagcgttca gcgtgacagg
    16621 actggactct tcacggccta cagtcatcag gttaaatttg agaatggtcc aacggaacac
    16681 aaagtaataa atgaagaaat aagccacttc aacacatacc gctaatggcc atcccgttct
    16741 tgtgccacgt aagacaccaa agctaataaa ttgaatcaga tcgccagtaa agcccatctt
    16801 cacgcccata tatggcagaa ccatgttagc caatccattc ataaaggcat ggaagacaaa
    16861 caacagcggg ctgacaaata agaacagaaa ttcaatcggc tcgctgacgc cgccaataat
    16921 taccgtgagc acaccggaaa tcagcaatgc tttgatagcc cttctgtttt gagcatgagc
    16981 agtgcgatac atcgccagcg cggctccagc cagaccatac tgaatcatca gtttgccctg
    17041 ctccattttc cccgccaaat ccagcggaat gaccgcattc tctttgacgt atgccataaa
    17101 catgttcagg gtgccgtaat actcttcacc gccaatcact gccgatccgc cgattggggt
    17161 aaagcggaaa actgatgtca ccaagtgatt taagccaaaa ggtatcgtca cgcgttcggc
    17221 aacagcatag aagaaataac caacaggacc ggaagttgaa atccagtgac caatgagcat
    17281 gaaaagattg aaaaatggcg gccagataaa aggaatgaac agaccaaatc cactcatcac
    17341 aatcagtgta atgataggca caaggcgtgg gccactataa aaccctaatg attcaggaat
    17401 acgtaagtta acgatctttt tatacatgct ggcgactaat aaccctgcca caatcccacc
    17461 caacacgctg gtattgtagg actggatccc gagtatgatg gtttgcccat gtgtcgacat
    17521 ttggtcagca gctaccaata agtcgtgctg tttaagataa aagttcgttc caagatgcat
    17581 cgccataaag ccaattaagc cagaaaaagc gccataggct ttatcatctt tctcttttaa
    17641 taatcccaaa ggtatagcta tcgcaaacaa tacaggtaaa ttaacgaagg ctaacaataa
    17701 aatcaagtat ggttttaatt aacggaatag ccaacactgg aatgaatttt cccatatcat
    17761 cgctggctaa accacttccc agccctaaca tcataccaca tacacttaac agagcgatgg
    17821 gatacataaa tgccttcccc aggctctgaa aaaaactcca ggctttcttt tgtttcatac
    17881 tggttatctc atgaaaatgt tatgtataat tagtccatta atactttgat gcgaatagaa
    17941 agatatagtt tctcttccag aagagaatca tatttaaaag ttgcgtacag atatctttca
    18001 atttcatgaa tggcacgttg taactgaaaa tctttttcaa gaaactgctc atagatatca
    18061 ccaatatttt tctgttttat tttttcatta cataacacac gactagaaaa atatttaagg
    18121 tgcatcataa atctggagta gttaaatgaa cttctgtcca cccttctgtt ataaacaact
    18181 tcaataagct ccgtgatttt ttgagacagc tcaataatcc ttactgattc ataatcaact
    18241 gaattttcat aattatttaa aatatgcaat gctataaaac caatctcatc atccggtagt
    18301 tgaacgctaa attgctcatt aatgattgtt aatgcatcac gacttattgc atactctgct
    18361 ttgtacaacg ctttgatttc ctcaaggaaa acattattaa tttgtatgcc tgagacgact
    18421 cgggatattg cattcgaaat gtgatcagca agaatcagta gtagcgatgg attgagtttt
    18481 ttttcgagat tactctccgc ataatgcact atcttttcgg caacaaaaat atactcaata
    18541 cccacatgct caatcaattt gtagagttta tttttttgct catttctgac ataaaaaatc
    18601 cggtttgcag gatgctcagg gaccgacata ccatattttt tattgtaacc aacgccaggc
    18661 ccagaaatga taacctcctg tccgttcacc gatgcctgta cacaattatt gttcatgact
    18721 ttttcgatga tcattccatg gctcccaaaa aaaaaggcaa ggcatgtcag aagtaataat
    18781 tacttcaaac attgccttgc ctgatttaac agttacaaga taattaacta atgcgtatac
    18841 cttacacgga attgtgctta ataaaagaga tgacaggctt attttttaag ccgatattca
    18901 aatattcgac agcagtc
//
LOCUS       DAZGTR010000086        18756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGTR010000086 DAZGTR010000000
VERSION     DAZGTR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.086"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            265..957
                     /gene="yieP"
                     /locus_tag="ACHLCY_003319"
     CDS             265..957
                     /gene="yieP"
                     /locus_tag="ACHLCY_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP0370508.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            980..2407
                     /gene="mdtD"
                     /locus_tag="ACHLCY_003320"
     CDS             980..2407
                     /gene="mdtD"
                     /locus_tag="ACHLCY_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP0370509.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2373..3365)
                     /gene="rbsR"
                     /locus_tag="ACHLCY_003321"
     CDS             complement(2373..3365)
                     /gene="rbsR"
                     /locus_tag="ACHLCY_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP0370510.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3369..4298)
                     /gene="rbsK"
                     /locus_tag="ACHLCY_003322"
     CDS             complement(3369..4298)
                     /gene="rbsK"
                     /locus_tag="ACHLCY_003322"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0370511.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4424..5314)
                     /gene="rbsB"
                     /locus_tag="ACHLCY_003323"
     CDS             complement(4424..5314)
                     /gene="rbsB"
                     /locus_tag="ACHLCY_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP0370512.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5339..6304)
                     /gene="rbsC"
                     /locus_tag="ACHLCY_003324"
     CDS             complement(5339..6304)
                     /gene="rbsC"
                     /locus_tag="ACHLCY_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP0370513.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6309..7814)
                     /gene="rbsA"
                     /locus_tag="ACHLCY_003325"
     CDS             complement(6309..7814)
                     /gene="rbsA"
                     /locus_tag="ACHLCY_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP0370514.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7822..8241)
                     /gene="rbsD"
                     /locus_tag="ACHLCY_003326"
     CDS             complement(7822..8241)
                     /gene="rbsD"
                     /locus_tag="ACHLCY_003326"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP0370515.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8408..10276)
                     /gene="kup"
                     /locus_tag="ACHLCY_003327"
     CDS             complement(8408..10276)
                     /gene="kup"
                     /locus_tag="ACHLCY_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP0370516.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10499..11995
                     /gene="ravA"
                     /locus_tag="ACHLCY_003328"
     CDS             10499..11995
                     /gene="ravA"
                     /locus_tag="ACHLCY_003328"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP0370517.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11989..13440
                     /gene="viaA"
                     /locus_tag="ACHLCY_003329"
     CDS             11989..13440
                     /gene="viaA"
                     /locus_tag="ACHLCY_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP0370518.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13445..14437)
                     /gene="asnA"
                     /locus_tag="ACHLCY_003330"
     CDS             complement(13445..14437)
                     /gene="asnA"
                     /locus_tag="ACHLCY_003330"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP0370519.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14589..15047
                     /gene="asnC"
                     /locus_tag="ACHLCY_003331"
     CDS             14589..15047
                     /gene="asnC"
                     /locus_tag="ACHLCY_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP0370520.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15137..15580
                     /gene="mioC"
                     /locus_tag="ACHLCY_003332"
     CDS             15137..15580
                     /gene="mioC"
                     /locus_tag="ACHLCY_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP0370521.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15958..17847
                     /gene="mnmG"
                     /locus_tag="ACHLCY_003333"
     CDS             15958..17847
                     /gene="mnmG"
                     /locus_tag="ACHLCY_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP0370522.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLERSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17911..18534
                     /gene="rsmG"
                     /locus_tag="ACHLCY_003334"
     CDS             17911..18534
                     /gene="rsmG"
                     /locus_tag="ACHLCY_003334"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP0370523.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4716 a         4871 c         4629 g         4540 t
ORIGIN      
        1 tttttgcttt ctctgtcgga attctcagga gaaccccgct gacccggcgg cgtgtttgcc
       61 gttgttccgt gtcagtggtg gcgcattata gggagttatt ccggcctgac aagaggaaat
      121 ttaaaataat tttctgaccg cgcatttttt cagcaaaagc cccgttaatg tgccataaaa
      181 caagcaattt gccgtttttg caaccgtaat cacacttcct ggtggcatac taaggaggaa
      241 taaaaagaaa aaggaagatt agtaatgcct ttaagcgcac aacagttagc tgcacagaaa
      301 aacctttcgt atgttctggc tgagaagctg gcgcaacgca tcttaaaagg tgaatatgaa
      361 cccggcacca ttttgcctgg tgagattgag ctgggcgagc aatttggagt gagtcgtaca
      421 gcggtacgcg aagcggtcaa aacgttaacg gcaaaaggga tggttttacc gcgaccgcga
      481 attggtaccc gggtcatgcc acaatcaaac tggaattttc ttgatcagga attgcttacc
      541 tggtggatga cagaagagaa ctttcatcag gtcatcgatc actttctggt tatgcgtatc
      601 tgtctggagc cgcaagcttg cctactggct gcaacggttg gcaccgcgga acagaaggcg
      661 catctcaata cgttaatggc cgaaatggcg gcattaaaag agaactttcg ccgtgaacgc
      721 tggatcgaag tcgatatggc ctggcatgag catatctatg aaatgagcgc caatccgttt
      781 ttgacctcat tcgcctcgct attccattcg gtttatcaca cttacttcac gtcaattacc
      841 agcgacacag tgataaagct ggatctgcac caggcgattg tcgatgcgat tgtccaaagc
      901 gatggcgacg cggcatttaa agcttgtcag gcgctgctac gctcacctga taagtgacaa
      961 cccgataata ggacattgaa tgagcgataa aaagaagcgc agtatggcgg gtttgccgtg
     1021 gatcgcggcg atggccttct tcatgcaggc acttgatgcc actattctga ataccgcctt
     1081 acccgcaatc gctcatagcc ttaatcgttc tcctctcgcg atgcaatcag ccatcatcag
     1141 ttatacgctg acggtggcga tgcttattcc ggtaagcgga tggctagccg atcgcttcgg
     1201 tacgcgtcgc atttttaccc ttgccgtgag tctgttcaca ttgggttctc tggcctgcgc
     1261 actttctaat tcgctaccac agctggttgt cttccgggtt attcagggga taggcggcgc
     1321 aatgatgatg cctgttgctc ggctggcctt actgcgcgct tatcctcgta atgaacttct
     1381 tccagtattg aattttgtcg ccatgccggg tctggtgggg ccaattttag gccccgttct
     1441 tggcggcgtg ctggtcacct gggcaacctg gcactggata tttttaatca atatccccat
     1501 aggtattgcg ggccttcttt acgcgcgcaa acatatgccc aatttcacca ccgcacgacg
     1561 cagattcgat atcactggct ttttgctgtt tggcctcagc cttgttctct tctcaagcgg
     1621 aatagagcta ttcggggaaa agattgtcgc cagctggatt gccttgacgg taattgtcac
     1681 cagcatcggg ttactgcttc tctatattct ccatgcgcga cacacgccaa acccattaat
     1741 ttcattagat ttatttaaaa cccgcacttt ctcgatcggt atcgtaggca atattgcaac
     1801 ccgtctgggg accggttgtg taccgttcct tatgccattg atgttacagg taggatttgg
     1861 ttatcaggcg tttattgccg gctgtatgat ggcaccgaca gcgttaggtt ccattattgc
     1921 aaaatcgatg gttacccaag tcttacgtcg tctgggctat cgccatacgt tagtggggat
     1981 cacggtgatt attgggctaa tgatcgctca gttctctttg caatcaccgg caatggcgat
     2041 atggatgctg atcttgccgt tgtttatatt agggatggct atgtcgacgc aatttaccgc
     2101 gatgaatacc atcacacttg ccgatctgac cgatgacaac gccagcagcg gtaacagtgt
     2161 tctggcggtc acgcagcaac tgtcgattag tttaggcgtt gctgtaagtg cggccgtcct
     2221 tcgcgtttat gaagggatgg aaggcacaac gactgtcgaa caattccact atacgtttat
     2281 cacgatgggc attattactg ttgcttcagc agcaatgttc atgcttctga aaacaaccga
     2341 tggtaataat ttgatcaaaa gacagcgtaa atctaagccg aaccgcgttc catcagaatc
     2401 ggagtaagtt gtaatcgttg ttgctgaagg gtcggctggg ttatccgatg gatgagtaca
     2461 tcaatcgcca gctcccccag ttcatctttc ggttggtgga tagtggttaa tggtggcgtc
     2521 ataaagcttg ccagttcgat atcgtcatag ccaatcaccg cgatatcctg cggaacctgt
     2581 aactctgcct gatataacgc ctggtaaacg ccaacagcca tagcgtcatt tccggtaaag
     2641 acggcctgag gacgcagcgg atgtgataac agttggcgca tggcgtcaaa cccgccgtta
     2701 aattcaaaat caccagtgac ttcatagcca tcaggaatgt tgagacccgc acgtttcatc
     2761 gccgcccgat aaccttccaa ccgcaggcgc gccggagttt tatccagcgg accggtaata
     2821 caggcgatac gggtatgacc tttatcgatc agatattgcg ttgctaagtc tccgcccagc
     2881 aacgagttat cctgaataag atcgctgtcg ccatcgaacg gagcccagtc catcatcaca
     2941 gtaggcactg tcggataacg ttgcatgatt tcacgcgaag gttgatgcgt ttcggtgcac
     3001 agtaacagca agccatcaac gcgtttttgc atcagcgttt ccagattgcg attcatccgc
     3061 tgttcatctc cttcggtatt gcaaaggacg agactataac cgcgttcgaa gcagctgcgt
     3121 tcaacgccac gcaccagttc tgaatagaaa ggattggtac tggcagtgat caacatgcca
     3181 atggtatgtg tttgattgag tttgaggcta cgcgccaggg ctgatggcgc gtaattgagt
     3241 tctttaatcg ccgcttcaac tttggcggta atcgcttcac tgacgaagcg atctttattg
     3301 ataacgtgag aaactgttga ggtagaaacg cccgccaggc gggcaacatc tttcattgta
     3361 gccaagcgtc acctctgcct gtctaaaaat gcgtcgatct cttcacgcca cggtacggaa
     3421 ggttgtgcgc ctttacgtgt tacggcaatc gcagcggcag catgggcaaa ccgaatcgcc
     3481 tctggcaatg gtttttcttc cagcaacgcc gtgattaacg cgccgttaaa ggtatctccg
     3541 gcagcaatgg tatcgacagc ctgcacccgg aatccaggaa cgcgctgacc ttcaccattc
     3601 acgctagccc atacaccacg acttcctaaa gtaatcagta cagtacggat acctttttca
     3661 tgcagtacct gcgccgcctt cgctgcatct tcatcatttt caacacgaat accggtgagc
     3721 ttttctgctt ccgtttcgtt tggcgtaata atgtccacca gcgccagcag ttcgtcagga
     3781 agttcgcgag ccggagccgg gttaagcgca acgatagtct tattttgatg ggcgattttc
     3841 gccgctgcca tcacactttc gagtggtgat tccagctgca ttaataatgc tgacgcgttg
     3901 gcaatacgct cacgttgcgc ttccaccagc gccggggaaa gggcagcatt agcgccggca
     3961 tgaataccga tgacattctc accttcgcca ttaacaaaaa tcagcgccac acctgttgat
     4021 tcgcctttga tcacgctgac cggagtaata tcaatgttat cagtggcgag ctgctggcga
     4081 acgctctcac caatgctgtc atcacccgta caggcaataa acgcgatatt cgcaccgcta
     4141 cgcccagcag ccacagcctg attcgcgcct ttgccgccaa atgcaacctg atagtggtta
     4201 ccggttacgg tttcgcctgg agtaggaaaa gattgaagat taagaatgtg gtcagcatta
     4261 atgctgccaa gaacaacgag gctgcctgcg ttttgcatat tcgggatgtc cataaaatgc
     4321 gccaccgtgt tagggtggcg cgtgccctgc ttttctttat attacacgtt catctgtcga
     4381 tgacgtattt atgtcaccat caggtcatac aacctgatta aaactactgc ttaacaacca
     4441 gtttcagatc aaccggatac ttagcctgaa ctttctcgcc tttcagcact ttatctgcgg
     4501 tttcgacgcc tttcgcgcca atctgatcgg gtagctgagc gatagtcgct gctagtttgc
     4561 catcattcac cgctttttcg ccatccggtg taccgtcaaa tccgacgacc atcacatccg
     4621 atttaccggc agtttgcagt gcgcgcagcg cgcccagcgc catttcatca ttctgcgcga
     4681 atacagcctg aacatccgga tgagcggtca acaggttctg cattacgttc aaacctttag
     4741 tgcgatcaaa atctgctggc tggctggcaa gaacattaaa cttgtgagca gcaacggcct
     4801 gctggaagcc ttcgccacgt tcacgggctg cggatgtacc agcaatgcct tgcagctcga
     4861 taactttggc accttcaccc gctttcttcg cgatgtaatc accggcgatt ttgccaccca
     4921 gtacgttatc agaagcaatg tggctcacca cttcaccttt cgttgcctgg cggtcaagag
     4981 tgataaccgg gatgttcgcc tggttagcca tcttcacagc attacctact gcgtcggagt
     5041 cggtcgggtt aatcagcaga attttggtgc cgcgaacggt taagtcctgc acgttcgcca
     5101 gctctttcgc cgggttgttc tgggagtcca gcaccaccag gttatagcca agcttatccg
     5161 cctctttctg cgcgccatct ttcagcgata caaagaacgg gttgttaagc gtggagacca
     5221 ccagcgcgat ggtgtctttt gccatcgcat tcgcactgac ggtggcgctt agcgcaacag
     5281 cggaaaccag ggtagccagt tttttcatgt tcatattcaa gatgtcctgt agtcgttatt
     5341 actgcttttt gttgtctacc agcaccgcca gcaaaatcac caccgctttg acgatcatct
     5401 ggtaatagga ggaaacacct aacaaattca atccattatt aaggaagcca agaattaatg
     5461 cgccgatcaa cgtcccaaca atgcgacctt ttccgcccgc cagactcgta ccgcccagaa
     5521 ccaccgcagc aatagcatcc agctcatagc cagtccccgc cgtgggttgt gcggaggaga
     5581 gacgcgccac ttcaatgatc ccggccagcg atgccagcag accacaaaga gaatagacga
     5641 tgattttgat tttattgacg ttgataccag aaagacgcgt tgccgcttcg ttgccgccca
     5701 gcgcgtagat gtaacgcccc agacgcgtgt gatgcagcat gtaccaggcc gcgaggaaga
     5761 caatccccat gatccagact ggcgtcggta cgcccagcgg acgaccaata ccaaaccagc
     5821 caaacagatc ggcgttctca gtaaagccgg tattcactgg gctaccgttg gtataaacca
     5881 tggtcacgcc gcgcagtaaa agcatcataa ccagcgtagc gataaacgcc tgaacgcgac
     5941 ctttcgctac aatcaccccg gttaccgcac caattgcggc acctaacgcg agagcggcag
     6001 cgacagccac cagcgcattg acttcaatgc cgacgataga tgcagcaact gcgccggtta
     6061 gcgccaacag agaacctaca gacaagtcga tgcccgacgt caggatcacc agcgtcatcc
     6121 cgaccgccat aatggcgttc accgaggttt gctggagaat attgaataag ttattgatgg
     6181 tgaaaaagtt cgggcttaac gtcgagacaa tcgcgatcag caccagcaga gcgataagcg
     6241 atttctgctc catcagccac gctttcgtga aataacggcg accagagaca gtctgggttg
     6301 tcattttttt actcctgatt cacgcgatta agcttgccca cagcggcagc cattaacact
     6361 tcctgggtgg cctgctcacg agtaaattcc ccgctgagat gcccttcatg catgacgatg
     6421 atgcgatcgc tcatgcctaa tacttctggc atctccgatg acaccagaat gatgctcaag
     6481 ccatcggctt tgaactggtt aatcagttga tagatctctt ttttcgcgcc gacatctacg
     6541 ccacgggtag gttcatcaag gatcaacact ttggggcgtg tcatcagacc gcgggcaatc
     6601 gccacttttt gctgattgcc accggaaagc agaccaattg cctgttccat cgacggtgtt
     6661 ttcacattaa acagacgaat gaaatcactc acagcctgct gttcatcggc atgcttcaaa
     6721 ctgccgccag cgcggctgaa gtagcgcagc gctgtcagcg acatgttctc ttttactgac
     6781 atgcccaaca ctaaaccgtc acgtttacgg tcttcggaga tatacacaat gccgtttgcc
     6841 aggccatcct gcggtgaacg ggtaacgact tcatgcccat ccagggtgac gtaaccgctg
     6901 gtgcgcggta gtgcgccgta gagcactttc atcagttcgg tacgacctgc gcccatcaaa
     6961 ccagagacgc caagaatttc gcctttgcgt aaagtaaaag agacatcgtt aacgccaggt
     7021 ccgcagagat tatcgacttt caggcggata tctcccggcg ctttgtccag gtgtggatat
     7081 tgatcttcca gcttgcggcc caccatcatc tcaatcagcg aatcttcggt cagtgatgcc
     7141 acttcgcgct cagcaataaa ttgcccgtca cgaaaaacgg taacgtcatc gcaaatctcg
     7201 aagatttctt tcatgcggtg ggagatatag acaataccgc ggccttgcga ttttagttcg
     7261 cggatgacgc ggaacaggga ttcggtttcg gtatcggtca gcgcatcggt cggttcatcc
     7321 ataatgatga ctttcgactc aaagctcagc actttggcga tttcaaccat ttgctggtca
     7381 ccgatggaaa gatcgcccac cagcttgtcg cttttaaagc gcaggttaag tttagccagc
     7441 aatttatccg cttcggcata catggttttc cagtcaattt tgccaaagcg attaacaaac
     7501 tcacgaccga ggaaaatgtt ttcggcaatg gtcaactgcg ggatcaggtt cagttcctga
     7561 tggataatcc caatcccggc ttcctgggaa gatttcggcc cggtaaatgt cgtttctttc
     7621 cccagccata aaagcgtgcc ggcatcgcga gcatagatac cagtaagcac tttcatcatg
     7681 gtggatttac ccgcgccgtt ttcgcccacc agcgccatca cgcggcccgg atagacattt
     7741 aacgctgcgc ccgagagggc ttttacgccc gggaaggctt tatcgatgcc tttaagctga
     7801 agtaatgctt ccatgacggc ctcagaacgt cacgccagca cagagaatga tattcgcata
     7861 cggagaacat tctccgctgc gaattaccgc ctgactttct gcggtttgtt gtttgaattg
     7921 ttcatgcgtg gtgtaacgaa tttcaatggt atttccctgg tgtttttgca gctgctcaag
     7981 gtgagtgagc aacgtttcgt ggagttgcgg attatgttgt ttgatctctt ccgcgataat
     8041 ggcagcctcg acctgcattt catttgtgac gacgcccagc acctgcataa aagaaggtac
     8101 accctgggtt aatgccatat cgatacgcgt tgtacttttg gggataggta aaccagcatc
     8161 acacaccacc agcgtatcgg tatgtcccag acgggagatc accgatgaaa tatcagaatt
     8221 aagaacggtg ccttttttca tttttttctc catcagcgaa acgtttcgct gacccgcagt
     8281 ttaatcctga ctgagttggg aaaaccatcg tgacgttacg gaaatgtgat caacctcgaa
     8341 acgttttaca tggtgattaa ccatgaaaac aaaaacgccc ccttatggag aaaggaggcg
     8401 tctggcgtta gatttcgacc tgagtaccca gttcgataac cctgtttggc ggaatttcaa
     8461 attgatctgg tgcacgcagc gcattacgtt gcagcagcaa gtacagcttg ccgcgcagac
     8521 gcaaatacca cgggcgtttg ccgaggatca acgactcatg cgacataaag aaggaggttt
     8581 ccatcatccg gcaacttaat ccttccagac cgcagcggtg gaaaacttct tccacgtttg
     8641 gcgtttctcg ccaaccataa cttgccacca cgcgccagaa agtgggcgac agttgttcaa
     8701 tctgtacccg acggacatta tggacatatg gcgcgtcttc ggtacgcaga gttaacagaa
     8761 tcacccgctc atgcaatacc ttgttatgtt taaggttatg catcagcgca aagggaatga
     8821 cgttgattgc acgcgacata tacaccgcgg tcccgggcac gcgaacaggc ggtgatttct
     8881 ccagcgaagc aatcatcgct tccagagagt taccatgttc atgcatccgc cgcagcaagc
     8941 ggaaacgctc gctcttccag gtggtcatca cgataaacat cacagtaccg aggctcaatg
     9001 gcaaccagcc gccggagagc agtttatcga ggttagcggt gaacaatgga atatcgacac
     9061 aaaggaaagc aatcaggatc agcgcaacaa aatacttatt ccagtgccag ttctgacgtg
     9121 ccacggtagt cgagagaata gacgtcagca ccatggttcc ggtcaccgca atcccgtacg
     9181 ccgccgccag gttgctggag tgctcaaagc tgacaatcac aatcacgacc gcgacataga
     9241 gcatccagtt cacaaacgga atatagattt gccctgactc catttcggag gtgtgaataa
     9301 tgcgcatcgg cgacaaatat cccagacgta ccgcctgacg cgtcaatgag aagacgccag
     9361 agataaccgc ctgcgaggca attaccgtcg ccagtgcggc gatgatcagc agcgggatca
     9421 gcgcccagtc cggtgccaac aggaagaacg ggttcttaat cgcttccggg ttctttaaca
     9481 acagcgctcc ctgaccgaag taattaaggg tcaaggaagg taatacgacg gtgaaccacg
     9541 ccaggcgaat agggaactta ccaaagtgcc ccatatcagc atacagcgcc tcgacccccg
     9601 taatcgacaa caccactgcc cctaatgcaa taaaagaaac cgttttgtat tcaaggaaga
     9661 aatgcaccgc ccacattgga ttcagcgcat gcagcacttc cgggttagca ataatgctac
     9721 gtaaccccag tcctgccaga atcaaaaacc aggtcagcat gatcggcgca aacagcttac
     9781 cgaccatagc cgtgccatgt ttttgaatca taaataataa agtgagaacg ataattgaga
     9841 ggggaactat ccaggtatcc agctgcgggg cgacgatttc cagaccttca atggctgaca
     9901 tcaccgaaat agcgggtgtt atgacgactt caccatagaa aaagctgccg ccgattagcc
     9961 ccataatcac cagcattgat gtggttcgcg ccgacgtatt acgcccggca agcgacatca
    10021 acgtcaggat ccccccctca ccggcgttat ctgcccgcat cacgaaggtg agatatttaa
    10081 tggaaaccac aaagattagc agccagaaga tcagcgataa aaagccaaac acggcatcgc
    10141 gttcaacgcc aaaaccaaac tggccggaca aacattcacg aagtgtatat aacgggctgg
    10201 taccaatatc gccgtagaca actccaatcg ccgcgagggt aatcgcgggc aatgattgct
    10261 tattatcagt gctcatagac tagtctttcg ttgaaatatg aaatgtgtgc ttagtccctt
    10321 ggcccacaaa aaagcgcaca gtatgcacga ttaacggcaa aatcgtactc ctaaatgcgg
    10381 ccacattaac ctggctcaaa gaaaaatacc ccccctttga gactatttta gccaccagca
    10441 agaaacgtct atactcgcaa tttacgcaga acttttgacg aaaggacgcc acttcattat
    10501 ggctcaccct catttattag cggaaagaat ttcccgcctg agcagttcgt tggaaaaggg
    10561 gctttatgaa cgtagccacg ccatccgctt gtgtttatta gcggcattaa gtggtgaaag
    10621 tgtgttcctc cttggcccgc caggtattgc caaaagtttg atcgcccggc gcttaaaatt
    10681 cgcctttcag aatgcccgcg cgtttgaata tctgatgacc cgcttctcca cgccggaaga
    10741 agtttttggt cccctttcta ttcaggcgct aaaagatgaa gggcgctatg aacgtttaac
    10801 cagcggttac ctgccggaag cagaaattgt ctttctggat gagatctgga aagcgggccc
    10861 ggcaattctt aataccttgc tcaccgccat taacgagcgc cagttccgca acggtgcaca
    10921 cgtagaaaaa atcccgatgc gtctgctggt ggcggcctcc aacgagctgc cggaagcaga
    10981 cagcagtctg gaagcgttat atgaccgcat gctgattcgt ctgtggttag ataaagtgca
    11041 ggataaagcg aatttccgct ccatgctgac cagtcaacag gatgaaaacg acaatccggt
    11101 tcctgatgcc ttgcaggtca cagatgaaga atatgaacgc tggcagaaag agattggtga
    11161 aattacgctg cccgatcatg tatttgagct gatttttatg ctgcgccagc aactggataa
    11221 attaccggat gcgccttatg tctcggatcg tcgctggaaa aaagcgatcc gattattgca
    11281 ggccagcgcc ttttttagcg gtcgcagtgc tgttgccccg gtagatctca ttttgctgaa
    11341 agattgcctg tggtatgacg cgcaaagcct gaatttgata caacaacaaa ttgatgtatt
    11401 gatgaccggt cacgcctggc aacagcaagg gatgttgact cgccttggcg cgattgtgca
    11461 acgtcacctg caactccagc agcaacaaag cgataaaaca gccttaacgg taattcgtct
    11521 gggcggcatt ttcagccgtc gtcagcagta tcaacttcct gttaacgtta ctgcttccac
    11581 tctgactcta ctgctgcaaa aaccgttaaa actgcatgat atggaagtgg ttcatatctc
    11641 ctttgagcgt agcgcgctgg aacagtggct gagcaaaggt ggtgaaattc gcggtaaact
    11701 gaacggtatc ggctttgccc agaaactgaa tctggaagtt gatagcgccc aacatcttgt
    11761 cgttcgcgat gtaagtttac aaggcagtac gctggcactt cccggttcat cggctgaagg
    11821 tctgcctggt gaaataaaac aacaactgga agagcttgaa agcgactggc gcaagcaaca
    11881 cgctttattc agcgaacagc aaaaatgtct gtttatccct ggcgactggt taggtcgcat
    11941 tgaagccagc ctacaggatg tcggtgcaca gattcgccag gcacaacaat gctaacgctg
    12001 gatacgctta atgtgatgct ggccgtcagc gaagagggat tgatcgaaga gatgatcatc
    12061 gcactgctgg cctcaccgca gctggcggtc ttctttgaaa aattcccacg cctgaaggcg
    12121 gcaatcactg atgatgttcc ccgctggcgt gaagcgctgc gcagtcggct gaaagatgcc
    12181 cgagtcccgc cggaactcac cgaagaggtg atgtgctatc agcaaagcca gctcctctcc
    12241 acgccacagt ttattgtgca gctaccacag atcctggact tactgcatcg tctgaattct
    12301 ccatgggcag aacaagcccg acagttggtt gatgctaaca gcacgatcac ttcagcgtta
    12361 cacacgcttt ttctccagcg ttggcgttta agtctgatcg tgcaagcaac gacgttaaat
    12421 caacagctat tagaagaaga acgcgaacaa ctgttgagtg aagttcagga acgcatgacg
    12481 ctgagcggac aacttgaacc gattctcgca gataacaata ctgcagctgg tcgtctgtgg
    12541 gatatgagcg ccggtcagct taaacgtggc gactatcagt tgattgtgaa atacggtgaa
    12601 tttcttaacg aacagccgga actgaaacgc ctggcagagc agctggggcg ttctcgggaa
    12661 gccaaatcaa taccgcgcaa cgatgcgcag atggaaacct tccgcaccat ggtgcgcgaa
    12721 ccggcgacgg ttcctgagca ggttgatggt ctgcaacaaa gcgatgatat tttacgtctt
    12781 ctgccgccag aactggcgac actagggata acggaactgg agtatgagtt ttaccgtcgg
    12841 ctggtggaaa aacagttgct cacctatcgc ctgcacggtg agtcgtggcg tgaaaaagtg
    12901 atcgaacgtc cggtggtaca taaagattac gatgaacagc cgcgcgggcc gtttattgtc
    12961 tgtgtggata cttccggctc aatgggcggc tttaatgaac agtgtgcgaa agcgttctgc
    13021 ctggccttga tgcgcattgc tctcgctgaa aaccggcgct gctatattat gctattttcc
    13081 accgagatcg tccgttatga gctttcaggc ccacaaggca tcgaacaagc aatccgtttt
    13141 ttaagccagc agtttcgtgg cggcaccgat cttgccagtt gttttcgcgc cattatggaa
    13201 cgcttgcaaa gcagggaatg gtttgatgcc gatgcggtgg tgatttctga ttttatcgct
    13261 cagcggttgc ctgacgacgt gacgagtaaa gtgaaagagc tgcagcgggt acatcagcat
    13321 cgctttcatg ccgtggcgat gtcggcacac ggcaaacccg gcatcatgcg cattttcgat
    13381 catatctggc gctttgatac cgggatgcga agccgcctgc tcagacgctg gcggcgataa
    13441 attattacag cagagaaggg acgctctcgc gaacagcagc tggccatact ccacactgaa
    13501 cctggccgat atgcggcagt tgcagcagca gcatggtcaa acgagactgg ccgataccgc
    13561 cgccgatggt ctgcggcatt tcaccgcgca gcagcgcctg atgccattcc agctgcagac
    13621 gatcttcatc accggtcagc gccaactgat gcttcaacgt gtcggcatcc acgcggatcc
    13681 ccatggaaga aagctcaaac gcatcttcca gtacagggtt ccataccaga atatcgccgt
    13741 tcagacccgc atggcccagc tctgacgggg tgctccagtc atcataatcc ggtgcgcgca
    13801 cgtcgtgacg atgaccatca ctcagcttgc cgccaatccc gacgaggaat accgcgccaa
    13861 gatctttcgc aatcgcccgc tcacgccctt tggcatcgag atccggataa cgagacagta
    13921 actcctggct gtgtacgaag tggatctgat ccggcaggaa cggtgccaaa ccaaactctt
    13981 cgctaactgc agcttcggtt gctttaattc ccgcccagat cgcctctacc gtgcttttca
    14041 gagtcgagaa ttgacgctca ccgtcgccca ttacgcgttc ccagtcccac tggtcaacat
    14101 agaccgagtg caacggagaa agacgctctt catcggggcg aagggctttc atgtgcgtgt
    14161 acagcccttc gcccgcgctg aagtcgtgtt gccctaaggt ctgacgtttc cacttcgcca
    14221 gtgaatgaac cacttcgaac tgggcatcag gcagagcttt cacttttacc tgcaccgctt
    14281 tttcacagcc cgacaagtta tcctgcgtgc catcccccac acggctaaga atcggcgcct
    14341 ggacttcgat cagccccaga cgttcttcca gttggcgaga aaagtgagat ttcacgaagc
    14401 taatttggcg ttgtttggca atgtaagcgg ttttcatttt ttatactcct gcgtcctgtt
    14461 gcttatgatt aagcaacaaa aaccgacaca catgcaataa tcattcaata aaaagcctaa
    14521 cacacttttg attcattaaa aaagaaggct aaaatagaat gaatcatcaa tctgcataag
    14581 aaaatcctat ggaaaattat ctgatcgaca atctggaccg tggcatcctg gaagcattaa
    14641 tgggcaatgc gcgcaccgct tacgccgaac tggcgaaaca atttggcgtc agtccgggga
    14701 cgattcacgt tcgagtagag aaaatgaagc aggcggggat cattaccggg gcgcgtattg
    14761 atgtcagccc gaagcagctc ggttatgacg taggctgctt tatcggcatt atattaaaga
    14821 gcgccaaaga ctacccttcc gcgctggcaa agctggaaag ccttgatgaa gtcactgaag
    14881 cctattacac aaccggccac tacagcatct ttataaaagt gatgtgccgt tcgatcgacg
    14941 ctctccagca tgtacttatc aacaagatcc aaacaattga tgaaattcag tccaccgaga
    15001 cattgatcgt cctgcaaaac ccgatcatgc gtaccatcaa gccataatcg gcttttttaa
    15061 tcccatactt ttccacaggt agatcccaac acgttcacag cgtacaatac gccactctta
    15121 ataaaggtgg cggtttatgg cagatatcac tcttatcagc ggcagcaccc tcggcggtgc
    15181 cgaatatgta gcagaacacc tggctgaaaa gctggaagag gcgggtttta ccaccgaaac
    15241 gctgcacggt ccgctgttgg aagatttatc tgcctcgggg atctggctga ttatcagctc
    15301 cacccacggt gccggagata ttccggacaa cctttctcct ttctatgaag cattgcagga
    15361 acagaagccc gatctttctg cggtccgctt tggcgcaatc ggtattggta gtcgtgaata
    15421 tgacaccttt tgtggggcta tcgataaact cgaggcagaa ctcaaaaatt ccggtgcaaa
    15481 acagacaggc gaaacactga agatcaacat tcttgatcac gacattccgg aagatccggc
    15541 agaagaatgg ctgggatcgt ggattaattt actcaaataa gtatacagat cgtgcgatct
    15601 actgtggata actctgtcag gaagcttgga tcaaccggta gttatccaag gaacaacggt
    15661 tgttcagttt ttgagttgtg tataacccct cattctgatc ccagcttata cggtccagga
    15721 tcaccgatca ttcacagtta atgatccttt ccaggttgtt gatcttaaaa gccggatcct
    15781 tgttatccac agggagtgcg atcctaataa gagatcacaa tagaacagat ctctaaataa
    15841 atagatcttc tttttaatac ccaggatccc aggtctttct caagccgaca aagttgagta
    15901 gaatccacgg cccgggcttc aatccatttt cataccgcgt tatgcgaggc aatcaccatg
    15961 ttttatccgg atccttttga cgtcatcatc attggcgggg gtcatgcagg caccgaggcc
    16021 gcgatggccg cggcgcgtat gggtcaacag actctgcttt tgacacacaa tatcgacact
    16081 ctggggcaga tgagctgcaa cccggcgatc ggcggtattg ggaagggaca tctggtaaaa
    16141 gaagtggatg cactcggcgg tctgatggcg aaagcgatcg atcaagcggg tatccagttt
    16201 aggatactaa acgcaagcaa aggaccggcg gttcgcgcta cccgagctca ggcggatcgt
    16261 gtgctctacc gtcaggcggt acgtacggcg ctggagaacc aaccgaacct gatgatcttc
    16321 cagcaggcgg ttgaagatct tattgtcgaa aacgatcgcg tggtcggtgc tgttacccaa
    16381 atgggactga agttccgtgc caaagccgtc gtgctcaccg ttgggacgtt cctcgacggt
    16441 aaaattcata tcggtctgga taattacagc ggtggccgtg ctggtgatcc gccgtccatt
    16501 ccgctttctc gccgtttgcg tgaactgccg ctgcgcgttg gtcgtctgaa aaccgggaca
    16561 ccaccgcgta ttgatgctcg aactatcgac tttagtgtgc tggcgcaaca gcatggcgat
    16621 aacccaatgc cggtattctc gtttatgggc aatgcgtccc agcatcccca gcaggtgccg
    16681 tgttatatca ctcataccaa cgagaaaacc catgatgtga tccgcagtaa cctcgagcgt
    16741 agcccaatgt atgcaggggt gatcgaaggt gtcggcccac gctactgccc gtcgatcgaa
    16801 gacaaagtta tgcgcttcgc cgacagaaat cagcatcaga tcttccttga accggaagga
    16861 ctgacctcta atgaaattta tccgaacggt atctccacca gcctgccgtt cgatgtgcag
    16921 atgcaaatcg tccgctctat gcaggggatg gaaaacgcga agatcgtgcg tccgggttat
    16981 gccattgagt atgacttctt cgatcctcgc gacctgaaac cgacgctgga gagcaagttt
    17041 atccaggggc tgttctttgc tggtcagatt aacggcacta ccggttacga agaagccgct
    17101 gcgcaaggtt tgctggccgg tcttaacgct gcccgtctgt ctgctgacaa agaaggttgg
    17161 gctccggcac gttctcaggc gtatctcggc gtactagttg atgacctgtg cactttagga
    17221 accaaagaac cgtatcgtat gtttacttcg cgcgcagaat atcgtctgat gctacgcgaa
    17281 gataatgcgg atctgcgttt gactgaaatc ggtcgtgaac tgggcctggt ggatgacgaa
    17341 cgttgggcgc gctttaacga gaaacttgag aatatcgagc gtgagcgtca gcgtctgaaa
    17401 tcgacctggg taaccccgtc ggcggaagct gcagccgaag tgaatgctca cctgactgcg
    17461 ccgctttccc gtgaagccag tggtgaagat ctgctgcgtc gtccggaaat gacttatgaa
    17521 aaattaacca cgctgacgcc gtttgcccct gcgttgacag acgaacaggc ggcggaacag
    17581 gttgagattc aggttaaata cgaaggttat atcgcgcgcc agcaagatga gatcgaaaag
    17641 cagctgcgta acgagaacac cctgctaccc gcgacactgg attaccgcca ggtatccggt
    17701 ctttctaacg aagtgatcgc caaacttaac gatcacaaac cagcctctat cggccaggct
    17761 tcgcgtattt ctggcgtcac gcctgcggcc atctccattc tgctggtgtg gctgaaaaaa
    17821 cagggtatgc tgcgtcgtag cgcataacgc attaaaaatg cctggtaagc acccgcttac
    17881 caggcaacgc atcaagaaca ggtaatcacc gtgctcaaca aactctcctt actgctgaaa
    17941 gacgcaggta tttcgcttac cgatcaccag aaaaaccagc ttattgccta cgtgaatatg
    18001 ctgcataaat ggaacaaagc gtacaacctg acttcggtcc gcgatcctaa tgagatgctg
    18061 gtacgccata ttctcgatag cattgtggtg gcaccgtatc tgcaaggtga acggtttatc
    18121 gatgtcggca ccggaccagg actgccaggc attccactct ctatcgtgcg tcctgaagcc
    18181 catttcactc tgttggatag ccttggtaaa cgcgtgcgtt tccttcgtca ggtgcaacat
    18241 gagcttaaac tggagaatat tgaaccagta cagagcaggg tagaagagtt tccttcagag
    18301 ccgccatttg atggcgtaat tagccgcgct tttgcctctc tgaacgatat ggtgagctgg
    18361 tgccaccatc ttcctggtga gcaaggccgt ttctacgcgc tgaaagggca aatgccggaa
    18421 gatgaaatcg ctttgttgcc cgaagaatat caggtcgaat cagtggttaa acttcaggtt
    18481 ccagccctgg atggcgaacg tcatctggtg gtgattaaag caaataaaat ttaattttta
    18541 tcaaaaaaat cataaaaaat tgaccggtta gactgttaac aacaaccagg ttttctactg
    18601 atataactgg ttacatttaa cgccacgttc actcttttgc atcaacaaga taacgtggct
    18661 ttttttggta agcagaaaat aagtcattag tgaaaatatc agtctgctaa aaatcggcgc
    18721 taagaaccat cattggctgt taaaacatta ttaaaa
//
LOCUS       DAZGTR010000087        18510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGTR010000087 DAZGTR010000000
VERSION     DAZGTR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.087"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(64..642)
                     /locus_tag="ACHLCY_003335"
     CDS             complement(64..642)
                     /locus_tag="ACHLCY_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0370524.1"
                     /translation="MTFRMSEQSRTIKIYNLLTGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWNLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     YGEYQKWNGTSWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVNTAVAADVEWPVAPQ"
     gene            complement(642..1496)
                     /locus_tag="ACHLCY_003336"
     CDS             complement(642..1496)
                     /locus_tag="ACHLCY_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370525.1"
                     /translation="MQGATGLELYEAYNNGYPTTYGNIIHLKGMTAVGEGELLIGWSG
                     ISGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPEEFYPVGAPIPWPSDTVPSGYALI
                     QGQTFDKSAYPKLAVAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASAS
                     STDLGTKTTSSFDYGTKTTSSFDYGTKTTNSAGAHSHNIPVGHTGAGNGVSAGYNAAL
                     GTGTTSSAGGHAHNVYIGAHNHTIGIGAHAHSVIIGPHGHTITVNATGNAENTVKNIA
                     FNYIVRLA"
     gene            complement(2061..2774)
                     /locus_tag="ACHLCY_003337"
                     /pseudo
     CDS             complement(2061..2774)
                     /locus_tag="ACHLCY_003337"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(2777..3307)
                     /locus_tag="ACHLCY_003338"
     CDS             complement(2777..3307)
                     /locus_tag="ACHLCY_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP0370526.1"
                     /translation="MSNSLLPPSASSFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(3300..4196)
                     /locus_tag="ACHLCY_003339"
     CDS             complement(3300..4196)
                     /locus_tag="ACHLCY_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0370527.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREAVS
                     AALELESEPLNVIAQTTAFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLT
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(4200..4550)
                     /locus_tag="ACHLCY_003340"
     CDS             complement(4200..4550)
                     /locus_tag="ACHLCY_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP0370528.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(4547..5113)
                     /locus_tag="ACHLCY_003341"
     CDS             complement(4547..5113)
                     /locus_tag="ACHLCY_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0370529.1"
                     /translation="MRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(5125..5760)
                     /locus_tag="ACHLCY_003342"
     CDS             complement(5125..5760)
                     /locus_tag="ACHLCY_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0370530.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELWGISQADERLIYNTVLGRIAEAVR"
     gene            complement(5753..6220)
                     /locus_tag="ACHLCY_003343"
     CDS             complement(5753..6220)
                     /locus_tag="ACHLCY_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0370531.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSPEGDWSHVFQDVTWGESDG"
     gene            complement(6358..6765)
                     /locus_tag="ACHLCY_003344"
     CDS             complement(6358..6765)
                     /locus_tag="ACHLCY_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFP0370532.1"
                     /translation="MKLWPTLGVAFLLIAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGGQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(6762..7154)
                     /locus_tag="ACHLCY_003345"
     CDS             complement(6762..7154)
                     /locus_tag="ACHLCY_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFP0370533.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQVSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(7151..7474)
                     /locus_tag="ACHLCY_003346"
     CDS             complement(7151..7474)
                     /locus_tag="ACHLCY_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0370534.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(7477..7677)
                     /locus_tag="ACHLCY_003347"
     CDS             complement(7477..7677)
                     /locus_tag="ACHLCY_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP0370535.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(7677..8171)
                     /locus_tag="ACHLCY_003348"
     CDS             complement(7677..8171)
                     /locus_tag="ACHLCY_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0370536.1"
                     /translation="MSMVARTEPRPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(8274..9074)
                     /gene="gpM"
                     /locus_tag="ACHLCY_003349"
     CDS             complement(8274..9074)
                     /gene="gpM"
                     /locus_tag="ACHLCY_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP0370537.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLTDADNMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(9120..10172)
                     /locus_tag="ACHLCY_003350"
     CDS             complement(9120..10172)
                     /locus_tag="ACHLCY_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0370538.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(10196..11032)
                     /locus_tag="ACHLCY_003351"
     CDS             complement(10196..11032)
                     /locus_tag="ACHLCY_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0370539.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSVQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11187..12938
                     /locus_tag="ACHLCY_003352"
     CDS             11187..12938
                     /locus_tag="ACHLCY_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0370540.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGHPDVMAAGKKG
                     RAKKLKNHFTPEQISALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRRRFSVDWNTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            12938..13984
                     /locus_tag="ACHLCY_003353"
     CDS             12938..13984
                     /locus_tag="ACHLCY_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0370541.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAYLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGSGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNSYALLDEKTAP"
     gene            complement(13999..14523)
                     /locus_tag="ACHLCY_003354"
     CDS             complement(13999..14523)
                     /locus_tag="ACHLCY_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370542.1"
                     /translation="MVINYKQLREKREQVKESFRRNEDLTPLVHLAQGIVDAYEISLE
                     LPSQTWTDSDGNRQHYVSCGLETTEGFRRMPLSQIPASTPKARGGNDERKLIFSIETV
                     VDDTPGEVAFVHTPLSIAMYNDEIQVRVNNNIVPLKEGNSPYTTVCEAIQYYVLSEID
                     NLKPDGTQKMVQLW"
     gene            complement(15720..16031)
                     /locus_tag="ACHLCY_003355"
     CDS             complement(15720..16031)
                     /locus_tag="ACHLCY_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0370543.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKTNPAEAYRLAAWADDEMLPVTQISSKKFEAQSAPVAKITSQMAGNIRALFP
                     E"
     gene            complement(16036..16995)
                     /locus_tag="ACHLCY_003356"
     CDS             complement(16036..16995)
                     /locus_tag="ACHLCY_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0370544.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGTQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDIANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYNSLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFITDGVPHFALVNGLYAMNKE"
     gene            complement(17072..>18510)
                     /locus_tag="ACHLCY_003357"
     CDS             complement(17072..>18510)
                     /locus_tag="ACHLCY_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0370545.1"
                     /translation="EDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVD
                     GIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQ
                     MARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELN
                     DYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPETEDSARENGFDLQGGPAAPW
                     TRGNNCPRVQETDNNGAEQPEERPAPWPQLPDGVEVNEWMRSLKRHERRALMRSLRDK
                     QAKNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSL
                     YVDGSIIAPQGYEIVRKPDTRPDSRITQLWQHLSRNHGVSSTEIRHNPVASYLAQLGA
                     SDPEAAARLASALQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERASRKADLLQ
                     GGLTSGNKKQTETGLTNPVNEQKTRSDI"
BASE COUNT         3852 a         5046 c         4966 g         4646 t
ORIGIN      
        1 gtgttgtatt gtataaatag agtacataaa gccatctata tttcaatcga tggctttttc
       61 tctttattgt ggggcgactg gccactcaac atccgctgct accgctgtat taacacgatt
      121 caacaatacc cggtatgtct tccatgcagc cagtaacgat gcctcttcct ccgttgcaat
      181 atccaaatct acggcatcct gaagtggcgc aatatgctcg ctggctacct gcatcaggct
      241 gttttttgtt tcttccgcct cccttatccg aaacagtttt tctgcttctg catctttcac
      301 ccaggatgtg ccgttccact tctgatactc tccatacggc gacaaccagg taacattttc
      361 cggtaatgga ccgagttcag aaataaataa cgcgtcgccg gaagccacgt catagacggt
      421 ttttccccga tggtcttcaa cgagattcca cgatgcctca tcactgttga aaacggccac
      481 gaagccagcc ggaatatctg gcggggcaat atcggtactg tttgcaggca gacctgtatg
      541 aggtggaatg tatgcatcac cttcaccaat aaactcattg gttccggtca gtagattata
      601 aatttttatg gtccgtgatt gttcactcat tctgaatgtc attatgcaag cctcacaata
      661 tagttaaatg cgatgttttt tacggtgttt tctgcgttcc ccgtagcatt aacggtgatg
      721 gtgtgtccgt ggggaccaat aatgacagaa tgggcatgag caccaatgcc gatagtgtgg
      781 ttatgggcac cgatatatac attgtgagca tgccctcctg cgctcgacgt ggtacctgta
      841 cctaacgcag cgttataacc ggctgataca ccattccccg cgccagtgtg accaacaggt
      901 atattgtgtg aatgagctcc tgcgctattt gtggtttttg tgccgtaatc aaatgaactg
      961 gtcgttttag taccgtaatc aaacgacgat gtggttttcg tccccaaatc agtactggat
     1021 gcgctggcgc tgtgggtgtg cgatttaatg ccgtcctgtt cctgagacaa tacggcacga
     1081 ccactggcag gtttgccctt gatagtccag ccacgcatat caggaataac gcctgacgga
     1141 taagcgactg caagtttcgg gtatgcagat ttgtcaaaag tctgcccctg tatcagggca
     1201 taaccagacg gaacggtatc tgatggccac ggaatcggtg caccgactgg ataaaactct
     1261 tcaggaggat gagccgaggt gtaaagctgc gcccacggcg accagtttgc gtcggtcgta
     1321 tcccgtcgtg aacgaataaa tgccggagca tgagcaccgc ttataccact ccagccgatg
     1381 agtaattcgc cttcgccaac ggctgtcatc cctttcaggt gaatgatatt tccatacgtt
     1441 gttggatatc cgttgttata cgcctcgtat aactccagac ctgtggcccc ctgcatatta
     1501 tctgtcaggg cggtcagccg accttttgaa gccagattaa ctgacgatac tgctgtccca
     1561 tctgacggta acgccccgat ctctgatgct gttggcttat tcctggagtt atagtccctt
     1621 cgccagccag gagcataatc atcaccatga ttaatataag taaattgagc attagggatg
     1681 ccttcaccgc tggatgtact cggtgtggta atgcgtatgg tcattgcgcc gcgggtgcca
     1741 ataacttcca caacagcacc tgcaagacaa atacttccgc aacctgtatc tgtaatgacc
     1801 ttattatttg cataagccca tgagcctttg cacatccagt aaggatggtt aaatgccccc
     1861 tgactctcta gccaaaaaat caactgcgca gttgtccatg cttcactatc tccaccaata
     1921 ttcagctctg cactatatgc acggcaggca ccgatatttt tcgtaaaggt gtctttatca
     1981 gagatatcct caccattctt agctttctgc agacgttttt cagcattgtc ataggcagct
     2041 tttactgcat ttgaagttgc cgccagcgtt tcagatgtac tgttggttgc actactaagc
     2101 tggacaaggc cttttcgcgc tgttgtggca tcctgcgctg tatattttcc gttagcaaga
     2161 tcatacgcga ccttaaccgc tttcggcgtt gcagccagcg tttcagaatc gctgttagtg
     2221 gcgctactga gttgaacaaa gccttttgcg gtcagcgagg catccgggtg acgtcgtgac
     2281 tgttcatgct ctttcagttt gttatccacg taatccactg tggccatcac catggtgtta
     2341 tccacggtaa gcgccacagt ggcagtgctg gatacggtca gaatggtgcg aaatgtttgt
     2401 gcacgtccgg acccttcggc aacggctggc ttgtaacttt cggcagtatt gcccaccgcg
     2461 attaaatcgc cgtgctcatc aaacacacca atttcccgga tccagaatcc gcccgtttct
     2521 ggaggaataa ccagctccgc aataatgcgg ttctgatgtg ttgcgtccag gatgacgcga
     2581 ttaacagtgt gtcgccacac ctcatgcacc agacgggtct gcttactgtc tggtgtgggc
     2641 aacgcgccgc caccgtcgcc cacggccata tgagtcaggc ggacaggctt accatctggc
     2701 gcggctgcct gagctaattt tttggcacct gtatcggtga taacggtttt aaattttcgt
     2761 gttgtggtac tcatgcttaa tcgtccggat aaatggtaat aacttcaccg tcataagttg
     2821 ccgccgctgc aaaaatatcc cccggaattt cctgaatgat attcagccct gtcatgtggc
     2881 ggctgaccgg acgggcatca gcaatcaacc gctccatttc cagatacatt tcctccgtca
     2941 cgccactgtc cagtgtgccg acttcaacgg taaatgttcc cggttctccg ccgaactccc
     3001 accactcaga cacgcgaatg aggtatccca gcggctcaat ggccctgcgc agtgcgctga
     3061 tggtcccttt gtgtcggtgt atcagccatg catcacgaat cacctgtcgt tttgtctctt
     3121 ccggccagtt gcgatcccag cggtcaacgg aaaatgccca ggcgagataa ggcagcaggt
     3181 gcaccgggca ggtgtccggc gaccacagcg tgttgaggtc taccgggatg tctgtaatgc
     3241 gcgttccgac agcttcggca caacgcatga aactgctggc tgatggtggt aacagtgaat
     3301 tactcattgc gcccaccttc gctgatggtg aacgactcac agcgcgccgc ctgtatgtcg
     3361 ctgatggcca tattctgtgt gggttcgatt atctccacgc gttgcacacc gtgcacatgc
     3421 agtgcggcag caatggcgga caacgccacg tcctgaccga taagcccctg ctcagccagc
     3481 cacttcctga acgacgattc agccgccgcc agaataggtt cggattccgg gccggggtaa
     3541 aagtacagtt ttgcattcag ccgccatgtc acgattctgg cgctctgtac agtaaggcgg
     3601 tcggccaccg ggcgggtatc ctctgcattc agaacggcgc gaacggtatt aagcaacgcc
     3661 tccgttgctg tgccgtcgcc ttcagtggac aggatggaaa ccgtcacatt tgccggagac
     3721 ggactgatag cccgcgcatc acgcaccaga ccgctggcgc tgcgggcaaa atactcgtat
     3781 gcacctgacg ggccagcaac actcaggcca tcgtacgccc gctgcgcccg cagtctcagc
     3841 gaggtgtcac tttccatcac tgcgtcggtg gtatccgttg ccggagtgat ggtcaggcgc
     3901 tttgtgttca tattgcccgc gaggttgtcc aggtctgtcc cggcgctgtg gcttaacatg
     3961 caggcgcgtg ccccctcatt gacccgctgg cgtaacagca tttcacgaaa cgctgttgtc
     4021 tgggcgataa cgttcagggg ttccgattcc agctccagcg cggcggagac ggcttcacgc
     4081 tgttcggcgg gataagccgc aatcatcatg gcctttgtgt cagccagaat tgcctcaaag
     4141 tcaggctccg cgatgatggc gggttccggt aactgggaaa ggtcaacggc gggcatgatt
     4201 tactccctca gcgtgatggt taactcaaca ttctgcatgg tctgcatgac agtgcccgac
     4261 agcgtcaccc cggcgcggcc tcccgctttc cagacaacgt cgatggcatc cagggcaatg
     4321 cggggttccc atcgtgtcag cgcaatcacg gcagcactca tgcattgcag acgcgtggtg
     4381 ttattcatgg gttcgtcaat caaatcaggc acaaggctgc catattcccg tcgcataacc
     4441 cggcttgcca gcggggtggt caggatatcc ctgactgact gtttcaggtg ctccatatcg
     4501 ttcaggtttc ccgtcccgtc cggattcatt cctgtgtagc gggttgtcac tgcgggcctc
     4561 ctgtcgaatc gctgccgcct ttcacgccac cgtgtttatg cgtatgcacg gtaatgccgt
     4621 ttgaggtgaa gttgccgccg ctgtgcgtga tattgccgct catctttcct ccttttgtga
     4681 cgtcaagcgt cgctgttctc agaaggtctg tgcattccac gacaggtgtg tccagcgtca
     4741 cactgacgga tgcctgcagg gtggccgttt tcatgccgct ggcgctcagt gcgcctgcgt
     4801 ccgcgtcgta gcggaacacc gcgccatccg gcgcgctgac cacgatttct ttcaggcttt
     4861 tgccgggtgc cggaatggca tcactccaca gactgccaat tatcatggcg gtttccgggt
     4921 tgccgccgat gcaggcaatt accacctgtt cgccgggtga tggcggcaac cacacattga
     4981 aggcacccgc gcgcgtggtg ttccagcgca gccagcctgt ttccagttcg ccgctgcgaa
     5041 cgcgcacgca ccaggattcc tcatcaactt cagagatgat cccggtacgg atgatgttgc
     5101 tcagcagtcg catgagttca gcgctcaccg tacagcctcc gcaatccggc ccagcaccgt
     5161 gttataaatc aggcgctcat ctgcctggct gataccccac agctcacgta ccgggtaatc
     5221 ggtgaaaatg cccggcgcaa cctgatcgcg ctcaccgaac tgatgaacgc gggcaatacg
     5281 tgcggccacg ccgctgtaac ccaccgtcac accggaagca tctgcacggg ctttcaggta
     5341 gcgggcggtg cgcagtttta cgaacatggg gacgcgcttt gtgctgtcct ggttgatgcg
     5401 ccgggtgcgt atttccagaa aacggtcgat gtcatcccgg taaaacgtgc ggatattgtt
     5461 tttatcctca tcccacccgg taatggttcg cccgtatttc cccgtgtcgt gatgccagtt
     5521 tttcagcgtg cgtgcttcgt tattccagat aaagcgaatg cgttcctgta tccgggttac
     5581 gcggcgtctg cgtggtgtcc acgcggtccc gtccggcgct ttctgtgacc ggatacgcgc
     5641 ctgctgggcg cggcgtaaat cctgtgccag ctttctggcg atgttattga tggcctgctg
     5701 attcaggctg tcgcggatgg cctcaaaggt ttcatccacg cgggtgaatg ccttatccat
     5761 cgctttcacc ccacgtcaca tcctggaata catgcgacca gtcgccttcg ggagatggca
     5821 ggcggggttt tggctccggc aggtgttctg cctgcggtgt cccctgactg ctgcgcgtga
     5881 tgcgaacgcg ttcccgcagg gggagcgtaa acaggagatc ggcgctgtca tcgtcattga
     5941 taacggcgga gaatttgatg tcctgattac gctcagggtt gagcaacaac tgtggctgat
     6001 tttcggataa ccacgccagc agcggcagcg tgaggtcgtc cagctccccg gcgtaatcca
     6061 tgacaaacat caccatctga tagcggtaaa caaacgatgg ggtttctccg gtcgtttcaa
     6121 tgttgccgct ctccacgaaa atggtgaatt tttccgggtt ggcctgacac catcggcatg
     6181 aacgggtcat ggcttcacgc agggaatcag ttttcagcat ggttgttatc ctcgttgttc
     6241 agtcgttgca gcctgcgctg ttccagtaat tcaatggccc gtttatccgc gttacaggtt
     6301 tccagtgcat ccagaaggcg gtcgccccat ataccgagat ttccccatgt gggagtatca
     6361 gggaagcggg gaggcgttac cggtatggtc agcgtctgcg gtataagccg gactgacggc
     6421 gctggcagtg gcgcgttctg cgtgcctgcg cagcctgtca gtaaaacgag cgtcaggcaa
     6481 agcgtgggcg cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc
     6541 gtcctgattg cgttgctgat tttccacgcg gagttgcgcc agcacctgct gcatatcctg
     6601 tcccccggcg ctgatgatat tcagggtgtc ggcggtactt ttcagggtgc tggcctgtgc
     6661 ttcgtttctg gcgttctccc ggcccagcga ccaagacaga cgcatggatg ttccccatcc
     6721 ggcaatcaga aggaaagcga cgccaagcgt gggccagagc ttcatgccgg ataggctccg
     6781 tgtggtaact gaaaatgcgg tccgtctttc agggtcttcc agtcgccgcc ccattccacc
     6841 ggaatattca gttcccggct ggcctgtctg aatgctgctg cgattttttc gtacagcggc
     6901 cattcccatg acacctggct gccgatataa gccacaacat ccacggcatg ccccgtaagg
     6961 tggcggctgt tcatggtctg gctcttaccc gtggccacca gttgcttctg gcggtaacgg
     7021 ctgcgcaacc cttcggtgat accaaaatcc acttccgaga tttccagtgc ccgtcgggtc
     7081 actttcacca gatcaggatt tacgccctgc aaattctttt cgctccggct gctgaattta
     7141 aatgtgttgc tcattcgtcc ttctccttca ccctgcgatt aaaggccgca ataaccttgt
     7201 cgcgtgcttt ctctgcaccc ataaaaccga ttgatgcgcc gataaacgtc acggcatctt
     7261 caggaaaccc gaagaagcgc aacgacccgg ccacggccat ggcaagaacg ccgcacgcca
     7321 gcgatcccgt tacggtctga accagtgttc gtccgtcata aagactcatc agcgcggaaa
     7381 tgctgaccgc cgcgcctact gcatacaccg ttggcaggtg gtcaaagagc cacgcaataa
     7441 cctgctctgt gatccctgtt tgaatggtgc tcactgctac tccccccaca actgaatcat
     7501 ttctcgtttc ttcttctccg gctccggcat ctcaacttcc tgcccggcgt ccagaaatac
     7561 ctgctgacag agtccggggt tggcatccag caccttttcg gtgacgccct gcgtcgtgcc
     7621 gtagtaccgg aaacagagcg aatccacggt gtcgccttcc agtgccttca ctttcatcag
     7681 cacaactccg caaagattcg cgggcggcac agaatgtcag agatggccca gctcacatca
     7741 cgccacaaat ccgatgtctg tatatccaga gcgtccgccc ggcggtcgcc cttgtccgtt
     7801 gtgtctgcat cacgataacg ctccagaatc agggcgcgcg tggcggtata aaccgcattg
     7861 cgccagtgcc agagattgac gctttctccg ttaattacag gggccggaac atcggccagc
     7921 gtctgatggc cagctgcctg ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt
     7981 gccacagccc cggtggcagt atgcagcagg cgggaggtgg tcacgcggcc cggcagtcgt
     8041 accgccagac gcagctcgcg cagcacaata tccggccaga atgcacccgc tgaaatgcgg
     8101 gtatcgccat catcggtatc ggtgatgtcg tcctctgcgg gtctgggttc agttctggca
     8161 accatactca tggggttcac tcctgaaaaa aatcgggcgg tgggtgcgcg gtgtaaacgg
     8221 tcacggagtc aaaccggaac accgcgcacg ccgcccgctg acggggtcag tcgttaaccg
     8281 cgcttcgcct tctgcgtcgc ggtggttttt cgtgttgcag gcttccgcgt tgtcttttta
     8341 cttttgctgc tttcgtcctg cgcctgctgt gcgctggcgt cttctggttc ggctgcggaa
     8401 tcggcttttt tcagggcgcg ggaaagggtt gcaatctcgc gtttcacacc tgcgttcggg
     8461 ttcaggtgca tcgcttcgcg cagcagcttc agtgataagg ccatgttgtc tgcatcggtc
     8521 agaccacgac gggcaaaggc gcacgccttg cataatttgg cgcgcacttc gtccggcatg
     8581 tcctggtcgg tgacaatctc ccggagggta tccagtggtt cgataaaggc ggataaatcc
     8641 gcgtcggcat ccgtcccggc ctgcgtcagt accgggttgc agatttcttc ggtcagtacc
     8701 gtggcagcag tacggccaaa gttatccggc ataatgaggt tatgacggac cacatacgca
     8761 ccaatacgca gcgccagcgg aagatcgccg cagtcaatcg cccacaccat cagcgtggca
     8821 atcacttcat cctgctgccc gccgtcagcc tccagcgttc cctcaatcca gccggaaaag
     8881 tccggcaata actctttttt gatggcggct ttcgcgcttc tggcctgtac gcccttaagg
     8941 cgggcctgtg ccagacgcag acgatacagc acctcttcat gcgcggtacg cgcggcgtgg
     9001 tccacgcctt cattcgcccg gcctgcgcgc tgtgccatca cgttctgcca gtgttgctgt
     9061 gcaggagtaa tcattttttc tctccgttac aggcgggcat gatgcccgcc gtgagttgat
     9121 tagctgtcgg cgaacttcag gccagtgacc atcgcgcact tgccatagtc ttcaacgaca
     9181 taagcgtcat tgatggactg gtaggtggcg atgcggttgt attccggttc gtctttcatc
     9241 aggcgacgca ttgaaccttt ctgccagtaa atcgacaggt tgttgaacga ggtgataagc
     9301 atcgttgcat ccgggaagaa cggcgcaagg aacacgccca gcccgccaat ggtgcgcgat
     9361 gacaggatga gctgcccggc aagtaattcc gcattgggat tctggccgct gatgctgttc
     9421 agcacgggca gacgcagcga gttaaacagg ttgcgcccca taatcaccac gaggtcgtca
     9481 gcttccttgt gccattcatc cagcagggat gagcgtgcgt cctgtaccag tgcatcagcg
     9541 ttcgcatact tacccgcgtg cgccacggtg ttgtccatgt tgcgggaggt cagcgtcacg
     9601 tcattcataa cgcgttcgct ggcgtcggtt ctgatgtgct ccagccagcc cacgttaacg
     9661 tcctgaagca gcttgttggt gctgaagttg gactcatccg cgtgagacgt gccgttgaaa
     9721 ccgatcatga tgcggtcaag cgccacctgt cgggcaatct gtgcgctgat gcgggactga
     9781 aaatcaggat gtgccgccca ggcatcaagc tgcggatatg aaataaacgt gtcgtagttc
     9841 acctgttcgc actggtattt gcggtttttc agatcaacca cgttattcgg gttacggcgt
     9901 tttgtgccgt cataactggt attcgtgcgc gcaatcggtc ctgtggtatc caggaggatt
     9961 ttttcgcctt tctggtcggt cacgccgatc acgttaattc tttttgtaaa ttcggtgctt
    10021 tcctttgagg cgttttcaaa acgctgctgc accgagggtt ctacggtaaa tcgcgatacc
    10081 agcgcagaaa ccgggatatt attaagcgac gcctgctgcg ccatatagca acccagcttg
    10141 ttgcgggtaa tatctgacat caccagattc ataaaaaatt tgctcctttg tcttatcaga
    10201 agtcagccag ctggtcggag gctgcgcccg ttgcggtgaa ccggttctgc ggatcgccgt
    10261 cctgcgtgcg cagtttttcc ttcagtgctg tcagctctgt ggtcagtgac gtaattttct
    10321 ggcggtcctg ctgatggcgg gtttccagca cattaaaacg gtcgataatg tcggcctgtg
    10381 acgttgcgac gccttccacc gcttcctgaa tacgggaaaa actggcgtca tccgctttgc
    10441 ggccacggcc aataatcccc attacgcggt taaaccactg ggtgccttct tcctggcgtt
    10501 gttctgccat ttcgatgatt tcagactcga tggcttcgga gataagcggc gcttcaccct
    10561 ggacactgtt gaacgtcatt accgcctgac gttgctgtgc cgtgaatttc aggcgctcag
    10621 tgcccaggct tgccggggtg tcggtcatcg ccagcccgac cagataggca cgcccgttaa
    10681 cggagaactg cgggtgcagt tcgatactgg aatagatttt cttgccgtcc gcgacaagct
    10741 gcttcatgcg ctcggtcggt tcgatttctg catacagcgc agtacgtccg gccagcggac
    10801 cttccgtaat gtcttccgta ctcagtgcgg tgacatcgcc cattgcggaa aattcgcttg
    10861 acgggcatgg cgagagatag tgctcaacgt tcacgcgggc agcgtaaaca tccgggttga
    10921 agttctcggc ggcttcacgc agatgcaccg gactgatttc acggccatca acagttgatc
    10981 cggagacagc cacgcgaaac tttttgcggg atgtcttttt ttcattagcc atagtttttg
    11041 cccctctgac tggttcttca gtcatgatgg caaagcgtaa caggctgata caaagggctt
    11101 ttgttgtaag aaaacggcca gaacaggggg ttaaggagaa cggtttcgcg cgcgggtaat
    11161 cttcctgtaa ttactcaggg ggagcaatga ttcaggacgc ttttgtgcgc cagcgtgcgc
    11221 ggcaacttta ctggcagggt tatccgcccg cagaaatatc acgtctgatg ggaataaacc
    11281 cgaacacgat ttatgcgtgg aaaaaacgcg accagtggga tgaaacgcca cccgtgcagc
    11341 gtgtcacgca gtccatcgat gcgcgcctca tccagcttac tgaaaaacag aataaaacag
    11401 gcggtgactt taaggaaata gacctgctga cccggcagct taaaaagctg catgatggcc
    11461 atccggatgt gatggccgca ggaaagaaag gccgggcgaa aaaactcaaa aatcatttca
    11521 cgccggaaca gatttccgca ctgcgggaaa aaatcatcag caggctggag tggcatcagc
    11581 ggggctggtt tgactccctg accctttgca gggaagccgg gatacgtaac aggatgatcc
    11641 tgaaatcccg acagattggg gcgacctggt attttgcaca ggaagctctg ctgatggcgc
    11701 tgcgtgacga tgtggcgcaa ccttaccagc gtaaccagat ttttttgtct gcgtcgcgtc
    11761 gtcaggcgtt ccagtttaaa agcattattc agaaggctgc ggctgaagtt gatgtggagc
    11821 tgaaaggggg cgataaaatc atcctctcca atggcgcaga gctgcatttt ctcggcactt
    11881 ctgctgcgtc ggcgcagtcc tatacgggca atttttattt tgatgaattt ttctgggtca
    11941 gtcgctttgc tgaactgcgc aaggtggctg gcgctatggc aaccctcagc ggactgcggc
    12001 gcacctactt ctccacgcca tccaccgaaa cgcacgaagc atacgcctac tggaacggcg
    12061 accgctggaa cgagaaaaag gccacgcata aacgccggcg tttttctgtg gactggaata
    12121 cgctgcataa cgggcttatc tgccctgacc ggacgtggcg gcaaattgtc acgctggaag
    12181 atgtggttaa tcacggctgg aaacacaccg atatcgacga aattcgtgat gaaaacaccg
    12241 aagacgagtt cctcaatctc tatatgtgtg agtttgtccg cgaaggggaa tcggcattta
    12301 acctgaatat cctgattggc tgcggtgttg acggatacga cgactggaaa gactggaaac
    12361 cttttgctcc ccgcccgatg gggaatcgtc cggtatggat tgggtatgac gcaaacggca
    12421 gcagtggcaa cggcgacagc ggcgctgtgt ctgtggtggt tcctccggct gttcctggtg
    12481 gccgttttcg aacggtggag acgcgacgcg ttcaggggct ggagtttgaa gaacaggcca
    12541 gagtcattga agagttcacg tgtcgctaca acgtggaaca catcggcatt gatgtgacgg
    12601 gcgggaacgg ggaggctgtt tatcagatag tgaaacggtt tttccctgct gctattccgt
    12661 acaccttcac gctgtcatca aaacggtcgc tggtactgaa aatgctgcaa ataatgcgtg
    12721 ccgggcggtg ggaatacgat cgcgccgaac gcgagctggt cgcggccttt aacgccgtgc
    12781 gtaaggtgaa aacaccgggc ggttttatca cttacgaaac ggaccgcgcg agggggatca
    12841 gccacggcga ccttgcgtgg gcaaccatgc ttgctgtcat taacgaacca attggcggcg
    12901 aaggagaaaa cgagcgtttc acggttatgg agttctgatg agcagaaaaa ataaaaaagt
    12961 gcgcatgagt tcacgcattg atctcgctga tgcgctcagg aaagaatcgt cgctcagtgc
    13021 attcactttt gatggtcctt atcgcctgac cgggcatgat ttgctggaca atatgtactg
    13081 tgctgataac gggcggtggt atgaaacccc ggtggactgg tacggtctgg caagagccgc
    13141 ccggcaaacg tcctggcatc agtctgcgct ttactttaag cgcaatgtat tgctcggctg
    13201 ctatattccg cacccgctgc tttcccggca ggatttctcg gcgctggcgc tggactggtt
    13261 tgtgttcggt aacgcatacc ttgagcttcg aagcaatatg ctcggcgaac cgcttaaatt
    13321 acggcacgca ctggcgaaat acatgcgacg cggaagcgat cttgaatcat ggtggtatgt
    13381 gcaggatggc aaggacgcgt ttcagtttcg ccctggcaaa gtgtgccacc tgatgaatcc
    13441 ggatattaac caggaaatct acggcatgcc ggaatatctc ggcgcattac tctcggccag
    13501 cctgtctcat tcggcggaca tgttcagaaa actgtattac gacaacggat cccacgccgg
    13561 gtgcatcatc tacatcggtg cagcgcaggt aaaccgcgaa agcatggact ccctgaaaga
    13621 aacattacag ggtgcacgtg gtagtggtgc atttaaaaac gtgctcattc atgcgcccaa
    13681 cgggggcaaa gagggggtgc aaattttgcc gttccagcag atcaccgcaa aagatgagtt
    13741 catgaatgtt aaggcggcat cccgtgatga tgtgctggct gcgcaccgtg ttccgccgca
    13801 actgatgggg gcgatgccgg gcgaaaaaag tgcgtttggt gatgtggaga aggccgcgcg
    13861 ggtttatgca attaacgagc tgatgcccgt tatggaggcc atgaagcaca tcaatgactg
    13921 gcttggcgaa gaggtgatcc gttttaattc ttatgctctt cttgatgaaa aaacagcccc
    13981 gtgatggggc tgtccttttt accagagttg aaccattttc tgggtgccgt caggcttgag
    14041 attatcaatt tcagagagaa cgtaatattg aatggcttca caaacggtgg tgtagggtga
    14101 attaccttct ttaagtggca cgatattatt attaacgcga acctgtattt catcgttata
    14161 cattgcgatc gaaagaggag tatgcacgaa ggcgacttcg ccaggtgtgt cgtcaaccac
    14221 tgtctcaatg ctgaaaatca gttttcgctc atcattgccg ccccttgctt tgggggtaga
    14281 ggcaggaatc tgagataaag gcattctgcg aaacccttca gttgtttcca gtccgcatga
    14341 aacgtaatgc tggcgattac cgtcgctatc agtccatgtc tgtgatggca gctccagaga
    14401 gatttcataa gcatctacaa ttccctgagc aaggtgtaca agcggggtca gatcttcatt
    14461 gcggcgaaaa ctttccttta cctgctctcg tttttctcgt aactgcttgt aattaatgac
    14521 cataaaacag cctccattga tttctttgct cgtattttgc acttatgaag tgtggtcggc
    14581 aaggtgccgc atcactgacg cgcttcgctt gtctgctgct tcgccggggc ataaaaaatt
    14641 tatgccccgg ctctccagct cctgtatcaa tcagataatt tcacgacgcc ttccagttta
    14701 tcgccaccat cgacggtcag actcttacgc aatcccaccg cgttgactgt atgttctcgc
    14761 tgcctcagtg cgattttgac ggccttacct ttcaccccat caaatcaaaa gccctcacgc
    14821 ctttttcatg ctcagcgtga gaaatatggc cattctgttg tgtctctgcg acatcgttca
    14881 gggaatgctg tttacccccc tgaaacgcgg gctgttcccc cgtcacctgc gcgtagaaaa
    14941 aacgcgtttt ttgtgcacgt acggatcctt gacggatcca gccgccacgc gggccggaag
    15001 tacaaaaagt cgttcaaaaa aattgtgcaa acttgtgcac tatcgtgcaa acaaaaaaaa
    15061 gcgccttatc ggcgcttcaa aaatatcaat tgttgctgtg tattaatcgc caaccacgaa
    15121 catatgcttc ataagcatct ctgtgcctta cagttccagc ctggctaaac ggaatgttag
    15181 ctaaaactaa atcattctga gccatagcgc gtccttcaag cgcatctttt atgcctagct
    15241 caaaagcaca ggcagcgcat ttgtgccgac cttcttgtcc ttgatattca gggagagaca
    15301 gaaatgttgg gttataacga tgagggttct tgcaaatacc tgttttagcc cgcactttat
    15361 ttacctcata ggaaaaatat gcgtgccttt acagaggtgc gtgtaagcaa taaaatatac
    15421 gacgcacatt ttgatttact tcggaaggca cgcatatcag gttaagtgag tccgaccgag
    15481 ttctacgccc caatagttgc tgctattggg gcgttttgca tggacaatgc cgcgcaatta
    15541 tcttgtcgct cacaatgcga acgatcttac aaaaaggcac attactgtca agataattga
    15601 tcgttttaat cgatagataa tagacaatct atttgtttaa cagatcgatt attgaagtaa
    15661 gtgcgccaaa tggaatgata ctgtttctat aaatgtgagc atttttcgcg cagatgcttt
    15721 tactcaggaa ataacgcccg gatattccca gccatctggc tggttatctt agccactggt
    15781 gcagactgtg cttcaaactt ttttgagctg atttgtgtca caggtaacat ctcatcatca
    15841 gcccatgcgg ccagtcggta agcctctgcc ggattcgtct tcagaagtgc cagcccggcc
    15901 agaaaagcca cgcgttggcc gcttttgcgg gcttctggtg taaggctgtc cagccaggcg
    15961 catgcttctc cttcgttctt gacggcagct ggcttcagat agaaacttat tcttctggtt
    16021 ggtgtcgtca ttggtttact ccttgttcat tgcgtacagc ccattaacca gagcaaaatg
    16081 tggcaccccg tccgtgatga aagtcgcatt aactccgcag gcttcgcgga tagcgggtgc
    16141 cacaatctcc gcccctccac cgacaaccat cacccgcccg taaccggaaa aacccgccag
    16201 cgcgcggatc actcgttgtt tcagtgtctc ctccttttca cgaataaccg ccatcaggct
    16261 gttgtaatgc gcgtcattgt gaatgtgctg gcgcagccag gcttcatcgt gacggtgctc
    16321 aataatggta ttggcgatgt ggtgactggt acgcataccg ttagtggcca tcaccgacag
    16381 cacggcatca gccatcaggg aaacgcctac gtgtggatcg caaaacacct ggctgatacc
    16441 tgccagctgc ccctgaacct ttgccacatc cagcgtggtt ccgcccaaat ccacaatcag
    16501 cagggattca aacggactca tgtcagccag tgccttaaag ccagccggaa tggattcagg
    16561 cataacccgc acgttacgga tagtgaatgc ttcgccgttc tggtactcca ccgggcgcat
    16621 aacgttcgct tttttgcggt tgatgttggc tatgtccggc tgtgcgtttg tgtcgaaata
    16681 ctcgctcagt ggcagggtga caaccacatc cacctcctgt ggcgtgatgc ctgatttgac
    16741 cagcgcgtga tgaatggcaa tgacattcac atcactgtat tggtattgcg tgtcggtcgt
    16801 ctggacaaag cgatcgctga ccggatcaaa accatagcgc acgccatcaa gcatgtagtt
    16861 cgcgggctgc gtgccaccga acggcgcaga ccattccgac ttgaagctgt tcgggctgat
    16921 ggcgttgcgg cgttcgccgt tctcagtcca tgccagcttg atgttggtgg agccgtcgtc
    16981 gatacaaatt ttcatgtcgc ttttccttat gttgattaat taatcgttta cgggattctg
    17041 aaatcccgtt tttgcctgtt ttgtgcgcgc ttcatatatc gctgcgcgtt ttttgctcat
    17101 ttacgggatt cgtgagtccc gtttctgtct gttttttgtt tccactggtc aggccaccct
    17161 gcagcaggtc tgctttgcgg ctggcgcgtt cagtggtttc actgattctc tgtgcgtgct
    17221 ctgcgtcgcg gatggcgcgc agcatgtcag aaagcacggt aacgggtgtt ttcatggtgt
    17281 tctgatcctg ctgaagtgcg gatgccaggc gtgcggcggc ttcggggtct gatgccccca
    17341 gctgtgccag atagctggcg accgggttat ggcggatctc cgtgctgctt acgccgtgat
    17401 tacggctcag gtgctgccag agctgcgtga ttcggctgtc cgggcgggta tccggtttgc
    17461 gtacaatttc atatccctgc ggtgcaatga tgctgccgtc aacgtacagg ctgccgcccc
    17521 gtagcaggtg ctgcatctgc tgttcaccga tatgcaggcc gagagattcg gcagactccc
    17581 gccattcttt agcgagtaat tcgtggttat caggcaaagg ccgctgctgt ttgcggctct
    17641 gtgtccagct ctgcatttca tcactgctgt tttttgcctg tttgtcacga agcgaacgca
    17701 tcagcgcccg gcgttcgtgc cgtttcagtg agcgcatcca ttcgttcact tcaacgccgt
    17761 cagggagctg cggccacggt gctggccgtt cttccggctg ttctgccccg ttgttgtccg
    17821 tttcctgtac acggggacag ttattgccac gagtccaagg ggcggcaggg ccgccctgaa
    17881 ggtcaaaacc attttcgcgg gcgctgtctt ccgtttccgg tttacgtctt accagtttcc
    17941 agttatccgg atgcgtgcac acacgggagg attccccgat gagtggtgac cagatcccgt
    18001 aaatctgtac gctctgttcg ccgtaatcat tcagctcatc tgcgaggtcg taggcggtgc
    18061 gaatcaggta gtctttgcgt ggaacaagta cgccgccctg tttttcaatg taggtggcaa
    18121 aacacccggc atcggcggca gcgagtaccg catccattgc atcatctttc agccgttgcg
    18181 ggccttccgg attgcgtgcc atctggctgg caaggcggcg cagttcacgc cacacctgac
    18241 gggaggggat gccaaagaac tggaactgac ggacccggtg aaggcgcgcc cagccgatgg
    18301 cgcgctccac gctctcggcc attgattttc cggtttcgtg gtcaacgcgt ggcttgcccg
    18361 ttttcgggtc gatgccatcc acggcgcggc tgtccaggtt ctttccgatg taggtggcga
    18421 tgtagctggt tggtgtgcct tttgagccgt cgacatactc cgccttaaaa cgcggagtta
    18481 tgtcatcgcc cagctcgtgg cggtcctcct
//
LOCUS       DAZGTR010000088        18361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGTR010000088 DAZGTR010000000
VERSION     DAZGTR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.088"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            155..796
                     /locus_tag="ACHLCY_003358"
     CDS             155..796
                     /locus_tag="ACHLCY_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HFP0370546.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPEHRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFTNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLRPLINIIHGTQTLAQKQVPQGELTGKGNYL"
     gene            1397..3106
                     /locus_tag="ACHLCY_003359"
     CDS             1397..3106
                     /locus_tag="ACHLCY_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0370547.1"
                     /translation="MADNKITLSSVRKALAGVFKDNGERDNILLSALAVHGGSGYLFS
                     RAGAPVQLSGFLGGKPGDSGMAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYST
                     IATALNIHITHALSFDKKTGQTFSIVPVHNGNDSDYDAAQGLCDELMNDIGRTINKEV
                     AGWAFIMSVFGVAYVRPYAKEGIGITSFECSYYTLPSFIKEFEVSGNLAGFSGDYLKD
                     ASGKMVFADPWTIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSL
                     LEYAYEPYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRA
                     ADLMEKRARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAA
                     ALGLDYTLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKY
                     GKVYPEGDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDA
                     FKRYLFSDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGN
                     DND"
     gene            3099..4118
                     /locus_tag="ACHLCY_003360"
     CDS             3099..4118
                     /locus_tag="ACHLCY_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HFP0370548.1"
                     /translation="MTDVLKTVTDRFCLYSNARKGRQNGRQYVLSAVKTMLESKETQE
                     GLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVIDNVPACRTVAISVDDNGIV
                     THTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIAVTTSFHGVDYVTTPNYISL
                     DHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHYGKMAELEMMVEATERTAEL
                     ETALLESQGRHLEAMAKIADAEARIALLEETAGIRNDVLAAMQDELDNLPIFVSAAQK
                     DAFRLKEPGDAKIVATLFESLIKVGARNLPVTKKLKEVPQAANVQAPRETSIITFNNS
                     INPFN"
     gene            complement(4194..5306)
                     /locus_tag="ACHLCY_003361"
     CDS             complement(4194..5306)
                     /locus_tag="ACHLCY_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP0370549.1"
                     /translation="MRTSLVTREEMIEAIEQHTICISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKINTPLVQES
                     ELVVVQSQPDEIPVLEWQGVRVVTTETLARGYGVDEANIRNNLSRNLDRFEEGKHYFL
                     LTGLKLREFKNRVTGSYSVGKNARSLTLWTERGAARMSKIVDTNEAWAFFEKLEDSYF
                     RQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSVA
                     KGILIGNFAKVVGLKQNALFVWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDD
                     EDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(5303..5524)
                     /locus_tag="ACHLCY_003362"
     CDS             complement(5303..5524)
                     /locus_tag="ACHLCY_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0370550.1"
                     /translation="MVNANPCARPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASVQEGL"
     gene            complement(6112..6669)
                     /locus_tag="ACHLCY_003363"
     CDS             complement(6112..6669)
                     /locus_tag="ACHLCY_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0370551.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLINE"
     gene            6839..7327
                     /locus_tag="ACHLCY_003364"
     CDS             6839..7327
                     /locus_tag="ACHLCY_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0370552.1"
                     /translation="MAQRGVNKVILIGTLGQDPEIRYIPNGGAVGRLSIATNESWRDK
                     QTGQQKEQTEWHKVVLFGKLAEIASEYLRKGSQVYIEGKLKTRKWTDDAGVERYTTEI
                     IVSQGGTMQMIGARRDDSQSSNGWGQSNQPQNHQQYSGGGKPQSNANNEPPMDFDDDI
                     PF"
     gene            7525..8202
                     /locus_tag="ACHLCY_003365"
     CDS             7525..8202
                     /locus_tag="ACHLCY_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFP0370553.1"
                     /translation="MNNMTTKELLTALPKYKSHKTVRASKIKDIEIIALMDVVLFCNI
                     EVVEPEGVKVHVDKIFLQKHRPEIGGYLVAYEDGSLSYSPEKTFEEGFSRTNDFFENG
                     VSLSIEGHNGVTFITARDVTISAGGIATLQEEIDLEAADFSDALMWLKDGKKVARRGW
                     NGENQFCWLVPEGQYPARMEAIKGYFPGDLVPYGAYFALKNAQGVVVPWVPSVGDLLA
                     CDWFVVE"
     gene            8209..8991
                     /locus_tag="ACHLCY_003366"
     CDS             8209..8991
                     /locus_tag="ACHLCY_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370554.1"
                     /translation="MENTKAIQYRLRNGQSVEVTINNDGVPGEKVSISDLAIEKTIMC
                     HLGFTEEVSKKHGVAIWSAMDTGMRRFITARTPGMTMMDLMQIAPLFECEPLDVFSNP
                     AICQQLYGEMKLAVTPIVLHEGSLAGVWKVERISSYMPFHVNGVITGENQPVSVIKSD
                     LKRAILEASCRVVGLGKQSYVSFPAGPEGPAEILIMDADLLWQIQFLIGKSIIRAEEL
                     DQYITCTMTDEVKSVAIANARNLCRAALTELQENTTEEVESD"
     gene            9238..9525
                     /locus_tag="ACHLCY_003367"
     CDS             9238..9525
                     /locus_tag="ACHLCY_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370555.1"
                     /translation="MEKLNNFLDKISTTNTRAGHTITRIAKLSDAGVSDDVIALQLSS
                     NSANGFRYTADEIKAYKKLHNDVKTKVGITSKQTKALIKDQELTFVALPQN"
     gene            complement(9729..10049)
                     /locus_tag="ACHLCY_003368"
     CDS             complement(9729..10049)
                     /locus_tag="ACHLCY_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370556.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVDGGDEDDDDDEEVFSDETVN
                     QLLY"
     gene            complement(10039..13026)
                     /locus_tag="ACHLCY_003369"
     CDS             complement(10039..13026)
                     /locus_tag="ACHLCY_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370557.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASAILVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIEHATEFGIRTEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDGIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSEMAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGYVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKADD
                     ARGILKAFKALQSRVNKHVGDIYGVTAEGRDILKEHGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKK
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRSMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRHKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            13126..14334
                     /locus_tag="ACHLCY_003370"
     CDS             13126..14334
                     /locus_tag="ACHLCY_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0370558.1"
                     /translation="MKQQVSFKFRLKPDGQQERQMRRFAGACRFVFNRALALQNENHE
                     AGKKYIPYTKMASWLVEWKKDTETEWLKDSPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGIVKNVTVSQSCGKWY
                     ISIQTEREVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVKYMSK
                     SAAGTISHPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(14374..14739)
                     /locus_tag="ACHLCY_003371"
     CDS             complement(14374..14739)
                     /locus_tag="ACHLCY_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HFP0370559.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGASGGSSGEEKQPSVHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            complement(14736..16655)
                     /locus_tag="ACHLCY_003372"
     CDS             complement(14736..16655)
                     /locus_tag="ACHLCY_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370560.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIHWCATDLLEAISSTRLQLHRTMQAFVRALNQKLNGTGISAGSD
                     KTGDVAQNGARAIGGAEIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNS
                     DTLIAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALV
                     DEITQLQADSDQKEDAMSDLADQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTE
                     IDQLNRTGGRETIAPAGMTGRHSRAMTDRLASIKNRMHMNGEVTLSNGASMKQFIEDG
                     EGYIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVE
                     PEPAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARY
                     GIATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALELATSSPDEF
                     RVMLKTQFQKTFPNIAFPGGAGTEKMVQSMINALQAEVGEITQPEPAPAQPDETVSEA
                     DAEANKAIEYLNNVMDMQSTDMAEIRNARGNVREAIAALQAAGRFEENEELVNGAARH
                     LADLLVAIQKAGVAA"
     gene            complement(16657..17259)
                     /locus_tag="ACHLCY_003373"
     CDS             complement(16657..17259)
                     /locus_tag="ACHLCY_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HFP0370561.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVRLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            complement(17246..17689)
                     /locus_tag="ACHLCY_003374"
     CDS             complement(17246..17689)
                     /locus_tag="ACHLCY_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0370562.1"
                     /translation="MIGWGVCALALALADRYLLKRKDITHLELGDVEIKPGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            complement(17686..18015)
                     /locus_tag="ACHLCY_003375"
     CDS             complement(17686..18015)
                     /locus_tag="ACHLCY_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0370563.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
     gene            17945..18280
                     /locus_tag="ACHLCY_003376"
                     /pseudo
     CDS             17945..18280
                     /locus_tag="ACHLCY_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         4754 a         4669 c         4315 g         4623 t
ORIGIN      
        1 actgacaagt gacttcagtc agaatcatca cacgcccggt acggatggat cccttttcaa
       61 atattccatg gacggcacag tctgagtacc gggcgctacc ttcagttgta ttgctaagcc
      121 gccgctggtg gcttttcttt tttgtagggg cgctatggat aagaaaatat gcgttgtttc
      181 gatgagcgtc ggcaaaccgg cgtcaatgac tgctgcatgg atcaacaacg agctgataat
      241 ggctgagcgg accagctacc ctgaacaccg ccgtgatatg gaactccagc tgctgcgcga
      301 attgcgtgag aaagaggaaa aaggttttat cgtgctggtg gaagaggaaa acagctttat
      361 taccggtcga gttggccagc gtgtaaggtt gcgcgatccc ttcacgaacg gcaggccggt
      421 actgattgaa gcaatgcaga tttacaagga gctggaacgc cagaaagcga tcaagttacc
      481 gcgcaaggaa tccggcaaat acatccttca ccaaagcatc ttcgattccg aacacgataa
      541 aaaaggcgat gaatttttca acatcaactg gagcgaaata acaacagagc atgttctgac
      601 attgttatgc tgtttcgcaa cggaatacaa caatgttgcc agcgccgact acatcagggc
      661 aatggctgga gaagttgagg cccgccagga accatcgtta ctaagacctc tgattaacat
      721 aattcacgga acccaaacac tggcgcaaaa acaagtacca caaggggaat tgacgggtaa
      781 aggtaattat ctctgaaaga tgcccggcga tagaccgggc aaaagggcgg aaagtttaat
      841 ctgcattcag gagcaatgcg ttatctatga tgatctgctc ccattcttcg aatgcccgat
      901 cgcggacccc ctggggaaca ctgtttgttt tgaaatcaac gactgtccgc catttcccgt
      961 ccggacggta catgcgcaga gctttactgc ccccttccct tcgcacctca acgttatgct
     1021 tgtcagcaaa ctcttgtaat gctcgtagcg tcccatgctt tactgtgtag tatcgctttt
     1081 tcaagttttc tctccagcct gtgccaaggc tttgacgtcg aaattatgtc ctattcggtt
     1141 ttattgtatc agatagtgtg gacatattat ttcacgtccg gcgtagtcgg tcatagcttt
     1201 tgccttccac cttttccacg ccgtatctaa tcggcgcccg atgaaatgtt attgcactaa
     1261 tatatttcaa tatttcgctg acacttgaac agcgaaacac ccccatagat agcttgttca
     1321 ttggtaatcc ttaacaagtg actagtgtta aattccgttc aaacacgatg cgaattattc
     1381 taattaaggt gcaatcttgg cagacaataa aatcacgcta tcctcggtca ggaaggcgct
     1441 ggcgggggtt tttaaagaca acggagaacg ggacaacatc ctcctgtccg cgctggctgt
     1501 gcacggcgga agtgggtatt tgttttctcg cgcaggggca ccggtacaac tgtccggctt
     1561 cttaggcggc aaaccgggcg atagtggcat ggctggcgat gggctggtgg acggaagtcg
     1621 ctttatcttt gatgaagttc aactgccgga agaccgcttg caacgctatc cgctactcga
     1681 agagatggcg gtttacagca cgatcgccac cgcgctgaac atccatatta cgcacgcgct
     1741 ctctttcgat aagaagaccg gacaaacctt ctctatcgtg ccggtacaca acggaaacga
     1801 tagtgactat gacgccgcgc aggggttgtg tgacgagctg atgaacgaca tcgggcgaac
     1861 catcaacaaa gaggtcgccg ggtgggcatt tatcatgtct gtatttgggg tggcttatgt
     1921 caggccatac gccaaagaag gcatagggat cacgtctttt gagtgctcct attacaccct
     1981 tccgagcttc atcaaggagt tcgaggtcag cggtaacctg gcgggattta gcggcgatta
     2041 tctgaaggac gcgtcaggga aaatggtttt cgccgatccg tggaccatta tccctatgaa
     2101 aatcccctac tggcggccta agtcaaacct tatgcctgtg cacactggcc ataaggctta
     2161 cagcctgctg gataatccgg aagagcgcac gccgattgaa acccagaatt acgggaccag
     2221 cttgctcgaa tacgcctacg agccgtacat gaacctgcgt tcggcgatcc gctcgctgaa
     2281 ggcaacgcgt tttaatgcgt cgaaaattga ccgaatcatc ggtctggcga tgaatagtct
     2341 ggatccggta aaagcagccg attattcacg caccattact cagacgctta aacgagcagc
     2401 tgacctgatg gaaaagcgcg cacgcggcgc gaataacatg cctacggtca ccaataccct
     2461 gctgcctatt atgggcgacg gcaaggggca gatgaccatt gatactcaga ccatccaggc
     2521 agacatcaac ggcattgaag acattctcac ttatatgcgc cagctggcgg cagcacttgg
     2581 cctcgattac accctcctgg ggtgggcaga tcaaatgtcc ggcgggcttg gtgaaggtgg
     2641 attcctgcgc acggcgattc aggccgccat gcgcgcctca tggatccagc agggcgtaga
     2701 agagttcatt cagcgggcta tcgatattca tcttgctttc aagtacggca aggtataccc
     2761 ggaaggtgat cgcccgtaca aaatcgaatt ccactccgtt aataccgctc tgcaacaaga
     2821 gcacaacgat aaccgcgact cgcaggcgaa ctacgccacc atcgttacgc aaatcctcga
     2881 tgccgtcagc aataacagcg tcctcgctaa ttccgatgca ttcaaacgtt acctgttcag
     2941 cgatgtgctg gagattgacg aaaaaatctc tgaagcactg gtgaacgaac tgaaagctaa
     3001 aagcgaggac gacgatcacc tgatggattc catcatcaaa acaccgccac aggaactggc
     3061 gcaaatcctt gaatcggtct ttaaagaggg aaacgataat gactgatgtt ttgaaaacgg
     3121 tcactgaccg cttttgtctc tatagcaatg ctcgaaaagg tcgccagaac gggcgacagt
     3181 atgtattaag cgcggtaaag accatgcttg aaagcaagga aactcaggaa ggtttacgcc
     3241 ttggtgagct tttcggctat tacggtcacg gtcgccgaca gctgaccggc aaactggaag
     3301 taccagaaac cagcgtgatc atggtggaag gtcgcccggt cgtaatcgac aatgttccag
     3361 cgtgccgaac agtggctata tctgttgacg acaacggcat cgttacccat acacaggaaa
     3421 ttcttaacac agagccgggt aaaattgtcg ccgcgatgat cgaaagccga gctggtggct
     3481 ggagctgggc cactggcggg cgcgagtccg ggaaaatcgc tgtaaccacc agcttccatg
     3541 gtgtggatta tgtgacaacg ccgaactata taagtctgga tcatcctgcc agcgccggaa
     3601 tgtttgaaag cgcggattct aaatctttac tggcagagtc cctggcggcg catgggtact
     3661 ccgacgagtc agtgcaggcc gttatatccc attacggcaa aatggctgaa ctggaaatga
     3721 tggtggaggc gacagagcgt acggcagaac tggagaccgc actactcgaa agccagggcc
     3781 gccacctcga agcaatggcc aagatcgcag atgctgaagc gcgaattgct ttgctggagg
     3841 aaacagcggg tatccgcaac gatgtgctgg cagcaatgca agacgaactg gataacctcc
     3901 cgatcttcgt ctccgccgcc caaaaagacg cattccgcct caaagaacct ggtgatgcaa
     3961 aaatcgttgc cacacttttc gaatctctga tcaaagttgg cgcacgcaac ttgcctgtca
     4021 ccaagaaact taaggaagtt ccgcaagcgg ctaacgtcca ggcaccgcgt gagacaagca
     4081 tcatcacgtt taataattca atcaatccgt ttaattgacc accaaaaata accccggcgg
     4141 ctgccggggt atctgaaatc actggcggcc gaaaggccgc ctttttaatt taatcactca
     4201 gccgctacag gctttaataa acctgcatcg agcagtttac gcgtcaacca ctgctggcct
     4261 ttacccgtta attggggcgt cagccgtatc tggtaaccat cttcatcatc cagcaccact
     4321 tctttcaccg tgaaataccc ggcgttgatg tattgctgga acggcacatt tttacgtcca
     4381 ccggacgcta tcaggatgcc gttctcccgt aaccagacaa acagcgcgtt ttgcttaagt
     4441 ccaacaacct ttgcaaaatt cccaatcagg atccctttag ctactgatac ccggtcggca
     4501 aaatcgactt taggggcggc ggccaccagc tgctgattta gctggtgggc tttctgttcc
     4561 agaagttgct tttgttcagc caactcggca gccaggcgca gagcttcggg aagcgtctga
     4621 gggattgcga ccggttgctg ttctttttgc cggaagtagc tgtcttccag tttttcaaag
     4681 aatgcccatg cttcatttgt gtccacgatc ttagacatgc gtgcagcgcc gcgctctgtc
     4741 cagagtgtaa ggcttctggc gttcttacca acagagtaac ttccggttac tctgttctta
     4801 aattctctta attttaaacc agttagaaga aagtaatgct taccttcttc aaagcggtca
     4861 aggttgcgag acaaattgtt gcgaatattg gcttcatcga ccccataccc tctagcaaga
     4921 gtttcggttg ttacgacacg cactccctgc cactctagaa cgggaatttc atccggctga
     4981 ctttgaacaa ccaccagttc tgattcctga actaaaggtg tattaatttt ttcagattta
     5041 acgttagtag ctttcattct gtgtgcctcc ttgcgtgctt cggctgcgac ggttgcgtaa
     5101 ttcagatgac cctgttcgag caggtattcg cggatatcag acaacaggat acggtgaacc
     5161 gcgttcttgt cctttctccg gtaaagttgt ttggtgatca tgaagtagtt ggcaataacg
     5221 cctggtatat ccctggtgct gatacagatt gtgtgctgtt caattgcctc gatcatctct
     5281 tcacgggtga ctaatgacgt tctcatagcc cctcctgaac agaagcgtta acagggaggc
     5341 accagtaact gagagaattg cgcgagtcag tggaaaaacg ggcagagaaa atgcagggag
     5401 catccggaag ctgagaacgt gcctcatctt ctgtcggtgc aataacgaag tgatagtggc
     5461 gtttctggca ggagtaaaag cgccagataa attcagggcg tgcgcaagga ttggcattaa
     5521 ccatagttac ggcctcattc gtaggtttaa caacctgcga cccgctgtca aacaggtggc
     5581 aggacgtgac agggttgaca gactggcacg aatgaaacca gcaggccgaa gcctccccat
     5641 cacgccccac cataattcgg gcgtaacgtg gtttacggac acaaaaatac cgcaatatcg
     5701 gaaatctgcg gttgtccgca ttcgtattca ggctgtcaaa cctggtcgca gaatttgcta
     5761 cgacggtgga actataagcc tgaacgacga tgagatcaat agagccgtgt tcgatggtag
     5821 gttatttgct ctaataaatc aaatttatta gagcaataat ggttaaatca tcttcgcctg
     5881 cgtgccatat atttgcgcac cgcgcggcgt ggacaatctg aagcggtttc tttctgctgc
     5941 atcaatctcg cagccatgct caaaaaagtc aggcacagcc gaagcccggc atataatagc
     6001 ggttccagtg gccacgtctc attgagcaca tataccgcca tgaaaatcga gtcgaaaact
     6061 atcgccgcca gcgataactt cattgtcgaa agtcggcgga gctgccggag tttattcatt
     6121 gatcagcccc gtcaggcaaa gctggcgttc tttttcacgg cgaatcttta aacctcgcag
     6181 gggcacgccg ttactgttca caaaatcagg gagatggtta cacatattca cccattcccc
     6241 tttctgcgcc cacttgtgga tggacgtttc gactcgcatg cctcgcgctt tgctgtagta
     6301 ggtccgtaag ctattgcatc ccatattgaa tgccgcgctt gtcattgcac tgaaggcatt
     6361 atcgggcatg tctttgcccc gaaagtgctg attaatacag cgttcagcga tcaggatatt
     6421 cttttcccaa tcagcggcga tttgctggtc ggtttttcgc acacccggcg ttaccccgtg
     6481 tgtattaccg atcccgtcag tccatacccc cgccgggcac atgtatgggt cacgtcggca
     6541 accttcagcg tttccgataa gctcaagccc cgcctggttg gttcgcacat tgccattacc
     6601 catcacgatg gtaatcatca ccgcgatagc gcaaattgca ccgcctcctg cggctgtttt
     6661 tcccttcata aagacctcat aagcgaattt tttacgctcc aggacaaaca cccattcaca
     6721 gccaataccg actgactcga tccctttaga aggcacagga taatgcaaat cacttgttag
     6781 ctacgtttca aagatataca ttattgctct aattaattta ttttattagg taagataagt
     6841 ggcacaacgc ggtgtaaaca aagtcatcct gattggtacc ctggggcaag acccggagat
     6901 caggtatata ccaaatggcg gagcggtcgg aagactcagc atcgcaacga atgaatcatg
     6961 gcgcgacaag caaacgggcc aacagaaaga gcaaacagaa tggcataaag tcgttttgtt
     7021 cggaaaactt gctgaaattg cgagtgaata tttacgaaaa ggttctcagg tctacatcga
     7081 agggaaactt aaaacccgta agtggacaga tgacgccggt gtagaacgtt acacgacgga
     7141 aattatcgtc agccagggcg gcaccatgca aatgatcggc gctcgccgtg acgattcaca
     7201 gtcctcaaat ggctgggggc aatcaaacca acctcaaaac caccagcaat acagtggtgg
     7261 cggtaaacct cagagcaacg ccaataacga acctccaatg gactttgacg acgatattcc
     7321 gttttgaatg tgtaaaaaac gactgaaaga aaagcggtgg tccagacgcc gacaaaagca
     7381 cgaactcgca aacaaacgcc aaagttggca atggcacgcg cttttcacga aaagaacacc
     7441 ccgagatatt gcgttcgctg gtgggaaaac attcctgacc cacctgaagg cgcaatacat
     7501 caggttttaa gcagaggaaa gactatgaat aacatgacaa cgaaagagct tttgacggca
     7561 cttccaaaat acaaaagcca taaaacagtt cgcgcctcaa aaatcaaaga tatcgaaatt
     7621 atcgccctaa tggatgtggt ccttttttgc aacatcgaag tcgttgagcc agaaggggta
     7681 aaagtccatg ttgataaaat atttttgcaa aaacaccgac cagaaattgg cggatatctg
     7741 gtcgcttatg aggacggatc tctgtcctat tcgccagaaa aaacatttga agaaggcttt
     7801 agtcggacta acgacttctt cgaaaatggg gtatcgctca gcattgaagg tcacaatggg
     7861 gtgacattca ttacagccag ggacgtaact atttctgctg gcggtatcgc tactctacaa
     7921 gaagaaatcg accttgaagc agccgacttt tctgacgcgc tgatgtggct gaaggatggc
     7981 aagaaagttg cccgacgcgg gtggaacggc gaaaaccaat tctgctggct ggttcctgaa
     8041 ggacagtacc cggcacgaat ggaagccatt aagggatatt tccccggcga cctcgttccg
     8101 tatggtgctt atttcgcctt aaaaaatgca caaggtgtag ttgttccgtg ggttccttct
     8161 gtaggcgact tactggcatg tgactggttt gtagtggagt gatttaacgt ggaaaatact
     8221 aaagcaattc aataccgcct gcgtaatggt cagagtgtcg aagtgaccat caataatgat
     8281 ggtgtgcctg gcgaaaaggt ttcgatctct gatctggcta tcgaaaaaac catcatgtgc
     8341 caccttggct ttactgaaga agtgagcaaa aagcatggtg tagcaatctg gagcgcaatg
     8401 gatactggta tgcgcagatt cattactgct cgtaccccag ggatgaccat gatggacctc
     8461 atgcagattg cgccgctgtt tgaatgtgag cctttagatg tattcagcaa tccagctatc
     8521 tgccagcagc tatatggtga gatgaaactc gcggttaccc ccattgtgct gcatgaagga
     8581 tcgcttgctg gtgtgtggaa agtggagcgt atttcaagct acatgccttt ccatgtcaac
     8641 ggcgtaatca ctggtgaaaa tcaacctgtt tccgttataa agtcagacct caagcgcgca
     8701 attcttgaag caagttgtcg agttgtcggc ctgggcaaac agtcttatgt ttccttcccg
     8761 gctggccctg aaggcccggc agaaattctg attatggatg ccgatctgct ctggcaaata
     8821 cagtttctga ttggcaaaag catcatccgc gctgaagaac tcgatcagta cattacctgc
     8881 acgatgacgg atgaagtcaa aagtgtggct atagccaatg cccggaacct atgtcgtgct
     8941 gcattaacag aactgcaaga aaacaccacg gaagaagtgg aaagtgatta aaaagaaccc
     9001 gccaattggc gggttcttta tttattgaat tagcattcaa ctcccgattg ttatagcgaa
     9061 cactatgtaa cagaataata ttattaacat tttagtaata agttgattat tcatcttctg
     9121 tattcgcaaa gcgtaagtat tgctcatagt ttcttattcc agtacattgt gattcgttga
     9181 ggcaaaagaa tagtggcaat tctactggca ataaactcaa gtaagaggtt gtaaacaatg
     9241 gaaaaactga acaatttctt agacaaaata tccaccacaa acactcgcgc aggccacact
     9301 attacccgca tagctaagct aagcgatgcg ggtgtcagtg atgacgtaat cgcacttcaa
     9361 ctgtccagta acagtgcaaa tggcttccga tatacagcag atgaaataaa agcgtataag
     9421 aagctccaca atgatgtaaa aactaaagtg ggaattacat ccaaacaaac aaaagcatta
     9481 atcaaagatc aagagctgac ttttgttgca ttgcctcaaa actaaatttg tttaaagcta
     9541 tagctaacaa agagttataa gagcgatagt gataaattta tcattgtcgc tcttataacc
     9601 ttgttaaaga taagaatata acccaaaaga tatacatggg ttattaaggc cataattatc
     9661 aggcaaataa catgaagtcg tctatgtgat ggatgaaatt tgaacaatca attctatgcc
     9721 aattcctgtc aatacaagag ttggtttact gtttcatcac taaaaacctc ttcatcgtca
     9781 tcatcatcct catcgccacc atctactgct ggccaatcaa caaaccagcc agcgtaaaga
     9841 tgcagcgttc ggagaacatc acttgcggga gcatcaaggg tgttaacgaa tcccatatag
     9901 ctattgggat ttgccccagc tatggcttca gcgatcatgt cctcggtaat gtcaccggag
     9961 ataatgctca aacgcccgga aacttcttca ttatcatcaa attcgataat ggcatctccg
    10021 cctaatggcg ctgcgatttt aatctgcatt atttagctcc tttgccacac ctaataacag
    10081 ttccagcaat ccgtcaccat tcatcagtga tgcggcagcg gcctctttgt catgatacaa
    10141 ctgaagagcc atagagaata cttccgttgc tgacgttttg gaaatagtcg gtgatttatg
    10201 ccgaattttc ccggtgttac ttactgaggc tggcgggtat accttcgcca tataaatatt
    10261 actcaatcga gatctgaagc accattcagg cttgccacgc ccaccgatat tgacgaaaga
    10321 tggcttatcc ccttcaacat tggccttcag gaatgaccgg gctttctcta acaaaccagg
    10381 gttactgtac tcaagatgat gacccagctc gtgccacagt gcacttgcat tttcatcgtt
    10441 caaattgaca gcaacaacac catttagatt tgcatatgcc cttccctggt ggtgaactac
    10501 ctttgataag gtcgatattt tcccgccggt caggcgataa atatcagcaa gttccttgcg
    10561 caggtctatc ccaccattct gtccagcgcg ggcttcttcc acttcttctg tgataaaaga
    10621 gtcggcccac tcaagagctt tttcttcaga tacggatgag tttgcgatcg cactgttcat
    10681 ggcagataac actttctcgt ggaccgaacc catacttcgc tgattcattt gccagcgtgt
    10741 ctgcgggtta tatgagaatc gcttaagtag ttggtcaagc tgctcaagtt cttcttcact
    10801 gacatacttt ttagcctcac caataatgcc agggagaata ttgccgttag gattaaacgc
    10861 tcgcgaaagg aagagtttca gcgcccccat gccctccgat gcttcaatat caccaataac
    10921 ccggttaaca atggccgcac tcttcggatt agcatccgcc aacgctctgg ctacgatttg
    10981 cagggacgat acgacctcac gctgcatatc agtcctgatc tcatcaataa actctggcgt
    11041 tatgccgtgc tctttaagga tatcccggcc ttccgccgtt accccataga tatcaccgac
    11101 atgtttatta acacgacttt gcaatgcctt aaatgccttc agaattccac gggcatcatc
    11161 cgctttacta acggccttcc tgaatgctgg caagaagtct gagttaacct cattttgttg
    11221 atcggcccac tgaatggagg cttctttcat ctcgtccaga gtcagatcac ccaacgcggt
    11281 atggtctgtg aatatgagcg acaacctctg aaccatttct gccaatggtg atgccgaatg
    11341 cgccgcgcta aggaatgctt tcaccctggt tgggcgaatg gaaaaccagt caatagctgg
    11401 tggcatatct ccgtttttta tcgcctgcgc tatctcgtca aagccatcgc gcccaaggga
    11461 agatgcgtga tttaacaagc cgcgaagtaa cgaattgctg ataccgaata atcggcacca
    11521 ttttttcacg tcggcaacag gcattcgaac aaaatgcgca agcacttgaa caagctgttc
    11581 atcctgggga tctgtacggg aaagcagcct gatcagatga ataatgtctt tgatgccgga
    11641 tgcccgatgt aatagcaaac tggtatatgg agcaacaccg ttgtaactac cgccggaagc
    11701 tgactcgaaa agaccgccgg atatcccttg cataccttcg ttttccagtt cctgagatac
    11761 ctggcgaagg atatcctgta acgacacata gccgccgcca aacatatccc ccagcgcctg
    11821 gccctggtgc tgtaactcat cattgatacg ttgagccatc aacttaaagg cggtggccat
    11881 acgcttcgcg ctacggttat tcgcgacgat gaacaacgcg agtgctttca cttccggggc
    11941 catttcgctg aacatatccc cctgagcaat aacatcggta atatgctggc ctgactcctt
    12001 cgattgcctt accaggtcta ccgcatcttt caatgccgcc agcgcctttt tatcgaggct
    12061 atccgctgtc tcaatgccat caacaatagt tgtcacagcc tgcttgtgcg cttctcctga
    12121 taaagcctgc atctggacaa aatcattggc tgccgcatta agcgccgtca gaacattacg
    12181 catatccgga tcaggttctt ctgcaaccat ccttaccagg cgcgcatcct tatatgcctt
    12241 ggcaaagatc gcgttttgta tacggtctac aagttgccgt gttggtcgcc catcttcagt
    12301 tacaaggcca gccgcctgtg tggcaccaac ttgcgtcata aatccgcgaa taaacgcgtc
    12361 attactgcgg ctaagcagat ctccgctttc tgacggatta aacagcgcca tcatcgccgg
    12421 tgttatgctg tcggcatcaa caaaagcctt ttcactggct gccatttcct gaagatcaga
    12481 aatatttgag tccttggcaa actgaacgcg gtcaaccttc gttaaccggc ggcgcaccag
    12541 taccggagcc gtcattgatt caaccttttc agtacgtatg ccgaattcgg tcgcatgttc
    12601 aatcaggtac tcccgatacc gatccgcatt accgtcctga taggctttaa tgatccccat
    12661 ggtacgtcca ttacctgact caactgcatt gtcctcacca attatcggtg cgccatggct
    12721 ggataatccg gaatcggtaa gctgggcagg acgcaaatcc ttggatatct ggttgacctg
    12781 aagaaggctg gatgcgcggg tccggtcgcg cggctgaagt tcctggggat agtctggatt
    12841 aattttccca tccagagtat tggataccaa aatagctgag gcatcgacga tatcaaacgc
    12901 tgtttttacc tcgtctccct tcgctgtcac cacatacgaa acccgcccat aatcgggcag
    12961 gttctttagc agctcgatca gcgtttctat gctggtggcc attaccacct gatcgcttaa
    13021 gctcatccct gttacgcctt atccaatgtg ccgcccccta cgctcctcat agtggggata
    13081 taaggcacag ttttcctccc ggctattttt gcttaaaata gtgacatgaa acaacaggtg
    13141 tcattcaaat tccggttaaa accagatggt caacaggagc gtcaaatgag gcgctttgcc
    13201 ggagcttgtc gtttcgtttt caaccgtgct ctggcgcttc agaatgagaa tcatgaggcc
    13261 ggtaaaaaat acatccctta cacgaaaatg gcttcctggt tggttgagtg gaaaaaagac
    13321 actgaaaccg aatggcttaa agattctccc tcacagccat tgcaacagtc actgaaagac
    13381 cttgagcggg cttacaaaaa cttcttccag aatcgggcag cttttccccg attcaaaaag
    13441 cggggacaga atgatgcatt ccgctacccg cagggtgtta agctcgatca ggaaaacagc
    13501 cgtatttttc tgccgaaact tggctggatg cgctaccgga atagccgtca ggtcacgggt
    13561 attgtgaaaa atgttactgt cagccagtcc tgcggtaagt ggtacatcag tattcagaca
    13621 gaaagagagg tatccactcc tgttcaccct tcagcatcaa tggtcggact ggatgctggc
    13681 gtggctaaac tcgccacgct gtcagatggc acagtctttg aacctgtaaa tagttttcag
    13741 aaaaaccaga agaagctggc gagacttcag cgacagttaa gccgcaaggt caaattcagc
    13801 aacaactggc agaagcagaa acgcaaaata cagcgactgc attcccgtat cgcaaatatc
    13861 cgcagggact accttcacaa agtcacaacg accgtcagca aaaaccacgc aatgattgtc
    13921 attgaggatt tgaaggttaa atacatgtca aagtcagcgg cgggaacgat aagtcatccg
    13981 ggtcgcaatg tccgggcaaa atcaggttta aaccgttcga tactggatca gggctggtat
    14041 gaaatgcgcc gccagcttga gtacaagcag ctctggcgtg gcggtcaggt gcttgctgtt
    14101 ccgccagcgt acacaagcca gcgttgcgcg tgctgtggtc atacagcgaa agaaaatcgc
    14161 ctgtcacaaa gtaaattcag atgccaggta tgtggatata cagcgaacgc cgatgtaaac
    14221 ggcgctcgta acattttagc ggcggggcac gccgtacttg cctgtggagg gatggtgcag
    14281 tcaggccgcc cgttgaagca ggaacccacc gaaatgattc aggcgacagc ctgaacgtag
    14341 caggaatcca cgcccttcgg ggcgtggagg atgtcacgct gcctctttaa tgttggcggc
    14401 tatccatgcc gccgtgtgct gtttaacctg gtccaggtcg atgtatgtgc caacatattg
    14461 actcaagtcc tgtaacgtac cgataaatgc atcagtgctc tgatcgacga atttatcagc
    14521 caggaaatca gcaaccagtt ttggcacacc atcatgtacc gaaggttgtt tttcctcgcc
    14581 actactgccg ccggacgcgc cgtaccccat ctgttgcatg atctggtcaa tttcatcgct
    14641 gatatccagc aactccatgc cactcgcggt agccgctttg gacatcagag catccaactt
    14701 atcgctgagg tccattaact caatagctga tagtgtcatg ccgctacccc cgctttctgg
    14761 attgctacca gcagatcagc caggtggcga gcagcaccgt taaccagctc ttcgttttcc
    14821 tcaaaacgtc cggcagcctg aagggctgca atcgcttccc ggacattgcc ccgggcgtta
    14881 cggatctccg ccatgtcagt gctttgcata tccatcacgt tattgagata ttcaatggct
    14941 ttattagcct ctgcatctgc ttcgctaacc gtttcatcag gctgtgccgg ggccggttct
    15001 ggctgagtaa tctcaccgac ttcggcctgc aatgcattga tcatgctctg caccattttc
    15061 tcggtgccag cgccccccgg aaacgcaata ttggggaaag ttttttgaaa ctgagtcttc
    15121 agcattacgc ggaactcgtc tggtgagctg gtggccagct ccagagcttt ttgtgcatat
    15181 ttgccaaacg gaccattagt aagtgtcttc gccaggaagt cgaaagaatc ctcgcgaggc
    15241 aataacttca ggtcgtactc actcatttgc tgatcagaaa gcggggtatc gtaagtagca
    15301 atgccgtagc gtgcatattc ataatacggg tcaccttcat cagggcgcgg cagaatcgct
    15361 ttattacctt caggtattgc gccaggggcc gccggacgca tttgcagggc atatcgatat
    15421 gcacctacag agacttctgg ttcaggcgaa gagctaccgg tatcctccgc tggttcaggt
    15481 tcgacgtttt ccggtttatg ttcttctggt tggaccagat attccgatac attacccgct
    15541 ttataggctt taaacagctt gccgatcgca tctgccatgt ccacaccctg tatggattta
    15601 gccttgatca tgtacacgct gccatccgaa tcggttaact ggatataccc ttcaccgtcc
    15661 tcaatgaatt gcttcattga tgcaccatta ctgagcgtca cttccccgtt catatgcata
    15721 cgatttttga tacttgcaag gcgatccgtc atcgcgcgag agtgtctacc agtcatcccc
    15781 gcaggagcaa tggtttcgcg cccaccagtt cgattgagct gatcaatctc cgtctgcaaa
    15841 cgctcattct cttcataaag agaatccgct tccgatgcaa cagcgttaat tttctgctcc
    15901 agatctacct tctgcccttc taccgctgcc acctgatccg cgaggtcgct catggcatcc
    15961 tctttctggt cactgtcagc ctgtagttgg gttatttcat caaccagggc ttttttcttc
    16021 ttctgcgcac gctggaattt tgccgagttt ttctctgcaa ggttggcaag tttcatggtg
    16081 acctgcgcca gcgtcatatc acgtccactc atcggagcaa cggtatgagt aacgtctttt
    16141 ttattcagta agaactggaa agcaatcagc gtatcgctat tggtgatccg gttttccgct
    16201 gtcgggctat gaaacagaat gctgatagtc tgaccatcac tgagcggaat aatggctggc
    16261 aggaccggca gcccgttaac gttacgtgcc cggccaattt cagccccgcc gatcgcgcgt
    16321 gcaccgttct gggccacgtc ccccgtttta tcactccccg cagagattcc ggtaccattc
    16381 agcttctggt tcaatgcccg gacaaatgcc tgcatggtcc ggtgtagctg caaacgagta
    16441 gaactaatcg cctccagtaa atccgtagca caccagtgga tcggcgtgtc atagaagaac
    16501 gtagcctcga tttcctccag ggtgttggat tccgtcatca gatagcggtc ctcaccggcc
    16561 attaatgcgc gatattcatc atcagtcact ggcgggggaa gcacgtcaag cccaggcttg
    16621 atcgtcaccc ctttattgat attgaactgt tccatgttaa tttcctgctt tcagttgctt
    16681 aagacggcgt ttgagttcgc catttcgggc cttttcgtta ttgagtcggc ctgtctcctt
    16741 atccagcttc gcccgcaaat cagtgatctg ctgttgattg aaagacaccg agttctgcgc
    16801 tgatttataa gcggcaacca cctgagcatt ccgctgtttt gcctcttgca ggcgctgaaa
    16861 gttggatttt actgccggtt tcttgtctac cggattggca acacgtttcg ctttggcgat
    16921 cagtgatttc tggaattttg cggagttttt gcgggccgct tgccccatga cggtaccaag
    16981 cgtcttgata tccggcgact gagcgttagg aatagctttt ccattcagcc tcacagacga
    17041 tatatcgcca gtatcgttta cctgtatggc aagaatttgt ccgtcgttaa gaaccagctt
    17101 tgcggtttta actttaacgc catctttcgt tgttgcgcgg ttgctggagt caacctcaat
    17161 taccgtaaca ccggttttat tgatcgccgc gataagggat ttcagcccct tttcattaac
    17221 ctggtcaaaa tcgaccgttg catacttatt tttcgtcatc tgacacatcc tgtgcgagat
    17281 ttattacgta acttctgcgg atttgctgag taacagggaa aatccgatac aacgggttaa
    17341 tgaacgagtc gccatgcgta accatgacgt tgaaatgcca cagccgttct cctttaccca
    17401 tatattcagt gggtatgtac aaccattcac tgttttcgcc ctgttcagcc gacgtcagac
    17461 aacgttgttc gccttcaatc actgtcgttg gcttctgaac atcgcggatc caatatctga
    17521 ccgttgcgcc gcgcaaaaac gggaatttag accggtattt gaacggcacc cggatgaaac
    17581 ccggtttaat ttccacatca ccaagttcta aatgcgtgat gtccttgcgt tttagcaaat
    17641 agcgatcggc taaggctaac gcaagagcgc atacacccca gccaatcatt tcccacctcc
    17701 ctttttcacc aaacttgtaa gaacattcag aatgctatcg atattcactc gtttcatccc
    17761 tgaaatcacc tcatgaccgt tattgctggc tatcgttacc attaagtacg taattgataa
    17821 ctcccagccc tcgtgttgcc ccaataggta cgccaccgcg ccagctgtca ctgcaacaaa
    17881 gatctccgta accaatccca acaaattgcc agactggcga ccgtctcgga catccatcag
    17941 gaacgtgcct atcccaccaa ttactgaaag caggagcgca atagcaactg gagctaattc
    18001 ctgtgtgtca agcacaagtt ccctcctacg ttgtcaggag gtaatggtat gcaaagtaac
    18061 ttctcaaccg gtcatgcaaa gacaacaagc ggaaaaagaa aagcaaagcc tgctacagct
    18121 cgtcagggat aaaactcagc tctgggactc acagttacgg ctaggcatta tttccgttca
    18181 gggtaaacaa aaattaaccg agtggattct ctatgcgcag aaagtcgaat ccacggatac
    18241 ctccatcctt ccagtaacgt ttcccgaaaa gccggaatga ggaaaggccc ggaatcgggc
    18301 cttaattaat atctataagc tcaacaaata agagctaaca attttgttaa tttcctctgg
    18361 a
//
LOCUS       DAZGTR010000089        18142 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGTR010000089 DAZGTR010000000
VERSION     DAZGTR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.089"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..390
                     /gene="waaH"
                     /locus_tag="ACHLCY_003377"
     CDS             <1..390
                     /gene="waaH"
                     /locus_tag="ACHLCY_003377"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0370564.1"
                     /translation="YLHNTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITI
                     YPEFHQQITYEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQAL
                     LWSFRLWQWRDKTRSHHRITRSAFNLR"
     gene            complement(377..1336)
                     /locus_tag="ACHLCY_003378"
     CDS             complement(377..1336)
                     /locus_tag="ACHLCY_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP0370565.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLNESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(1340..2599)
                     /gene="envC"
                     /locus_tag="ACHLCY_003379"
     CDS             complement(1340..2599)
                     /gene="envC"
                     /locus_tag="ACHLCY_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP0370566.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(2633..4177)
                     /gene="gpmM"
                     /locus_tag="ACHLCY_003380"
                     /gene_synonym="pgmI"
     CDS             complement(2633..4177)
                     /gene="gpmM"
                     /locus_tag="ACHLCY_003380"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP0370567.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            4422..4853
                     /locus_tag="ACHLCY_003381"
     CDS             4422..4853
                     /locus_tag="ACHLCY_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP0370568.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4995..5246
                     /gene="grxC"
                     /locus_tag="ACHLCY_003382"
     CDS             4995..5246
                     /gene="grxC"
                     /locus_tag="ACHLCY_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP0370569.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            5309..5776
                     /gene="secB"
                     /locus_tag="ACHLCY_003383"
     CDS             5309..5776
                     /gene="secB"
                     /locus_tag="ACHLCY_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP0370570.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            5776..6795
                     /gene="gpsA"
                     /locus_tag="ACHLCY_003384"
     CDS             5776..6795
                     /gene="gpsA"
                     /locus_tag="ACHLCY_003384"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP0370571.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            6875..7696
                     /gene="cysE"
                     /locus_tag="ACHLCY_003385"
     CDS             6875..7696
                     /gene="cysE"
                     /locus_tag="ACHLCY_003385"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP0370572.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(7749..8222)
                     /gene="trmL"
                     /locus_tag="ACHLCY_003386"
     CDS             complement(7749..8222)
                     /gene="trmL"
                     /locus_tag="ACHLCY_003386"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP0370573.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(8408..9598)
                     /gene="lldD"
                     /locus_tag="ACHLCY_003387"
     CDS             complement(8408..9598)
                     /gene="lldD"
                     /locus_tag="ACHLCY_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP0370574.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            complement(9595..10371)
                     /gene="lldR"
                     /locus_tag="ACHLCY_003388"
     CDS             complement(9595..10371)
                     /gene="lldR"
                     /locus_tag="ACHLCY_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP0370575.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            complement(10371..12026)
                     /gene="lldP"
                     /locus_tag="ACHLCY_003389"
     CDS             complement(10371..12026)
                     /gene="lldP"
                     /locus_tag="ACHLCY_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0370576.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            complement(12395..17245)
                     /gene="ehaG"
                     /locus_tag="ACHLCY_003390"
     CDS             complement(12395..17245)
                     /gene="ehaG"
                     /locus_tag="ACHLCY_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP0370577.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIVNAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TSGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            complement(17245..17987)
                     /locus_tag="ACHLCY_003391"
                     /pseudo
     CDS             complement(17245..17987)
                     /locus_tag="ACHLCY_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4172 a         4725 c         4685 g         4560 t
ORIGIN      
        1 tatctgcata atacgtcagt gagtcggttg catagacaag ggaataaaaa ccttaattat
       61 caacgtcact atattaagat tacccgcctg ctggagaaat taaatcgaaa ttatgccgac
      121 aaaattacga tttatccgga atttcatcag caaataactt acgaagcatt gcgtgtttgc
      181 catgcggtgc gcaaagagcc ggatattctt acccgccaac ggatgattgc cgagatattt
      241 acttccggta tgtataagcg cctgattacc aatgtgcgca gcgtgaaggt cggttaccag
      301 gcgttactgt ggtctttccg cttatggcaa tggcgcgaca aaacgcggtc gcaccatcgc
      361 attacgcgta gcgcctttaa tttgcgctag cgttaaactt cgccgcttcg ggctgtttgc
      421 cccagccttg ccactgatgc tggaagtaaa cgatcagcgt gctctggctg atgctttcgt
      481 ttaatacttc aaagaagcga ttagcataaa caggttctat cggtttcttc ggtttgcaca
      541 gcttcacgcc acggaacgga ttacgcggtg catcaggcac agcgtttttc ggcggtgtag
      601 aagtatcaac ctgcggttca ttcagcaagc tgctggcttt caccagcgta atgtctggcg
      661 gcaggttata aaccatctgt tgtagcacgc gtaccgttga agggtgagga tgcccaatgg
      721 caatggttga accgttgcga cgcgccagat caattgcgcg attaaattgc acacggatgt
      781 ccgcttcgtt ctgcgaatcg tcgaggaaca ccttccgttt gatcaccttc acgccagtac
      841 cttgcgcggc gcgcatcgcc tgggtattac cgatggttac gctgtcgagg aagtaaagat
      901 tgtaacgctc cagtgcctgc atcactttct gcataccaaa caggttagag gtcatcttgc
      961 tacccatgtg gttgttgatc cccacggcat agggcacgtt attgaccgca ctacgaataa
     1021 tgcgctcaat ttcgtcgctg ctcatctccg ggcgcagcgt atttttctcc agcggctgtt
     1081 tactcagtgg tgccatcggg agatgaatca acacttcgtg cccgctgtta tgcgctttgg
     1141 tcgccatctc tctggcgtgc ggtgaatcgg gtaatacagc gacggagata gcggagggca
     1201 tcgccagcac ctggttttcg ttgtgcgggc gatacccaaa atcatcaatg acgatggcaa
     1261 gtttgccagc aagtacgggg gaggagagcg ccaacagagc ggcaaatgca agaacgttac
     1321 gacgaaatgg aaacaaaact tatcttccca accacggctg tggattgacc gcctgaccct
     1381 ggcggcgaat ttcgaaatag agtgaaggcc gaccctgacc gccactgctg cccaccagtg
     1441 caattggctg gcccgcgcga acctgcgaac caacgctcac cagtgcgctc tgattatagc
     1501 cgtaaagact catgtcgcct ttaccgtgct caaccaccac caccagaccg taaccttgca
     1561 gccagtcagc cagaatcacc cgaccgtcgg caatcgcttt aacttcagta ccttcagagg
     1621 caccgataac cattcctttc cagcgtagtt cgccctgtag ctgttcacca tagcgatgca
     1681 gcgtcggccc gcgaaccggc cagaatgcct gaccacgcgg cgcacccagg ccaccagttc
     1741 gggacatcag cgatttttcg ctttcggtcg gtttataggt ggtgcctttg cgcgtcgctt
     1801 ctttctggcg gtcgcgaacc gcctgggcct cacgcgcttc tcgttcagca cgcgctttcg
     1861 ccgcggcttc cgcacgggca atgctgttac gcagacggga ttcgttggca cgcagttcac
     1921 ttagctgctg ctgaccttgc tggatggaag attccagccc cgccagcgtc tttttacgct
     1981 cgttcagagc ctgagtcagt ttcgcctgtt gggcgcgctg ttcatataac agcgtttgtt
     2041 gctcgctttg tttctcttcc agttcagcac gctgcatggc gacttcttca cgcgtttgct
     2101 tcaactgggc aatggtttct tgtcgcgcct ggttgagata gccgaaataa gcctgtaaac
     2161 gctgtccacg ctggctttct tcaccgctga gaattagctg aataccggta tgctcgccct
     2221 gacggaatgc ggcatccagt tgcgcggcga ggctgcgctc ctgggcggct ttttgctgct
     2281 ccagtttggc aatcgacgcg ttcatctcat caatctgttt attgagttga ttgagcgtgt
     2341 tttgcgtttc gcgcagctta cgggtggctt cagagatcgc ttcttcctgc tttttcaatt
     2401 gtgcgagcag gcttgcgcgt tgttgttgct tttggcgtac cgcgcgctct tttgcggcga
     2461 tatcggcctg aatagatttg agttggtcac gctcatccgc gtgggcggaa aaggcgcaca
     2521 acaatacgcc agcgctaaga acgctggcgt agatgatggg cctgattgca aacctgcgcg
     2581 gtttcacggc ccgtgtcatg gtattaatcg cctttcccct catggggagg gattattcca
     2641 cgatgaacag cggcttacca gtcatctctt gcgggatttc catacccatc agcgacaaca
     2701 tggtcggcgc gatgtcagaa agtttgccgc cttcaaccgc tttcacgttc ttatcaccaa
     2761 cataaatcag cggaactggc aggttggtgt gtgccgtgtg tgcctgaccc gttgccggat
     2821 cgcgcatctg ttcagcgtta ccgtggtcag cggtgatcag cagttgtcca cccacggact
     2881 caaccgcttt cgcgacttct tccacgcagt gatccagtgc ttcaaccgct ttaaccgccg
     2941 cttccatcac cccggtgtga cctaccatgt cgccgttcgg atagttacag ataatggtgt
     3001 cgtatttgcc gctcttgatg gccgcaacca gtttttcggt cagctctgcg gagctcattt
     3061 ccggttgcag atcgtaggta gccactttcg gtgagttgat cagaatgcga tcttcgcctt
     3121 tgaacgactc ttctacgcca ccgttgaaga agaaagtaac gtgggcatat ttttcggttt
     3181 cggaaatacg caactgagtt ttgtcgtttt tcgccatcca ctcgccgaag gtgttaacca
     3241 gggaagcggg tgggtaagca accgcagttt tgatgtcagc ggcgtattcg gtcagcatca
     3301 cgaaatcgac gttaaccact ttcttacgcg cgaagccatc gaaatcagcg ttcacgaatg
     3361 cacgagtgat ttcacgcgcg cggtcagcac ggaagttcat gaaaatcagc gcatcgccgt
     3421 cttccattgc cgcatctggc tgaccttctg cacgaataac ggtcgctttc acgaactcgt
     3481 cgttttcgtc gcgagcataa gctgcctgca aaccagcaac ggcagtatcg gcctggaact
     3541 caccctgcgc cagagtcagc aggtcatagg ctttttcaac gcgatcccaa cggttatcgc
     3601 ggtccatcgc gtagtaacga ccaatgatgg acgctacgcg gcctttaccc agcgcagcaa
     3661 atttctcttc aaattttttc agcgaggatt cagcactgcg cggcggggtg tcgcgaccgt
     3721 caaggaatgc gtgcaggtag attttttctg cgccgcgttc agctgccagt tctaccatcg
     3781 ccatgatgtg atcttcgtgg ctgtgtacgc cgcctgcgga gagcagaccc ataatgtgta
     3841 ctgctttacc tgcgtttttc gctttatcta ccgcgccagt cagcactgga ttagcaaaga
     3901 aagcgcgatc tttgatttca acgtccagac gagtcaggtc ctgatacacg atgcggccgg
     3961 cacccaggtt aacgtggcct acttcggagt tacccatctg acggtcaggc agaccgactt
     4021 ccagaccgga agcgtcgatc agggtatgcg gacgattggc ccacagtgca tccattaccg
     4081 gggttttagc actaaaaatg gcgttatcct gctgttcttc acgatagcca tagccatcca
     4141 gaatcaccag taccataggt tttttagaaa ccgacattgc gacaacctca tactcaagag
     4201 tcaaaatttg cgtaatttta ctacagctga atcgataaaa tagcctctga agatcaaaat
     4261 atacgaccgc tcgcggcaag ccgatgccag atttgcgctt ttttttcgtc gtgccccgca
     4321 aaattgcatt ccagttaacg cgctggctgt atttgccgca ccgcgcaggt atactccttt
     4381 cctggttttt ttaatcacta cgtcgggagt tgttaccccc catgcaagaa attatgcaat
     4441 ttgttggccg tcatcccata ctgagtatcg cctggatcgc gttactggtg gcggttcttg
     4501 tgactacgtt taagagcctg acctcgaaag tgaaggtgat tactcgtggt gaagctacgc
     4561 gtctgatcaa caaagaagac gctgtggttg tggatttacg tcagcgtgat gacttccgta
     4621 aaggccatat cgcaggttct atcaatctgt tgccgagtga aatcaaagcc aacaatgttg
     4681 gtgagcttga gaagcacaaa gacaaaccgg ttatcgtggt agacggttct ggcatgcagt
     4741 gccaggagcc tgcaaacgcg ctgacgaaag ctggttttgc gcaagtattc gtgctgaaag
     4801 agggcgttgc tggctgggct ggcgaaaact tgcctctggt gcgcggcaaa taatttacct
     4861 gtcgtctttc atgatgcagg ccgttagctg caattaggcc cccagcgact tattgttata
     4921 agcttctggg gacactccat tttgcagccg ccctgcaaca tgaaatttca gggtaaaaaa
     4981 acaggagcta aattatggcc aatgttgaaa tctataccaa agaaacctgc ccgtattgcc
     5041 atcgtgcaaa agcactgctg agcagcaagg gcgtgagttt tcaggagttg ccgatcgatg
     5101 gcaatgccgc caagcgtgaa gagatgatca aacgcagcgg tcgcaccacg gttccccaga
     5161 tttttattga cgcacagcac attggcggct gtgatgactt gtatgcattg gatgcacgtg
     5221 gtggactgga tcccctgctg aaataacgtg tgaacgttgg cattacattg cgcagtattt
     5281 aaggacaaca cttaagggtt ttctacacat gtcagaacaa aacaacactg aaatgacttt
     5341 ccagatccaa cgtatttata ccaaggatat ctctttcgaa gcgccgaacg cgccgcacgt
     5401 tttccagaaa gattggcaac cagaagttaa acttgatctg gatacggcat cttcccaact
     5461 ggcagatgac gtatacgaag tggtactgcg tgttaccgta acggcctccc tgggcgaaga
     5521 aaccgcgttc ctgtgtgaag ttcagcaggg cggtattttc tccatcgcgg gtatcgaagg
     5581 cacccagatg gcacattgcc tgggcgcata ctgcccgaac attctgttcc cgtatgctcg
     5641 tgagtgcatc accagcatgg tatcccgcgg tacattcccg caactgaacc ttgcgccggt
     5701 taacttcgat gcgctgttca tgaactattt gcagcagcag gctggcgaag gtactgaaga
     5761 acatcaggat gcctgatgaa ccaacgtaat gcttcaatga ctgtgatcgg tgccggctcg
     5821 tacggcaccg ctcttgccat taccctggca agaaatggcc acgaggttgt cctctggggc
     5881 catgaccctg aacatatcgc aacgcttgaa cgcgaccgct gtaacgccgc gtttctcccc
     5941 gatgtgcctt ttcccgatac gctccatctt gaaagcgatc tcgccactgc gctggcagcc
     6001 agccgtaata ttctcgtcgt cgtacccagc catgtctttg gtgaagtgct gcgccagatt
     6061 aaaccgctga tgcgtcctga tgcgcgtctg gtgtgggcga ccaaagggct ggaagcggaa
     6121 accggacgtc tgttacagga cgtggcgcgt gaggccttag gcgatcaaat tccgctggcg
     6181 gttatctctg gcccaacgtt tgcgaaagaa ctggcggcag gtttaccgac ggctatttcg
     6241 ttggcctcta ccgaccagac ctttgccgat gatctccaac aattgctgca ctgtggcaaa
     6301 agtttccgcg tttacagcaa cccggatttc attggcgtgc agcttggcgg cgcggtgaaa
     6361 aacgtcattg ccattggcgc ggggatgtct gacggtatcg gttttggtgc gaatgcgcgt
     6421 acggcgctga tcacccgtgg gctggctgaa atgtcgcgtc ttggtgcggc gctgggtgcc
     6481 gatcctgcca cctttatggg catggcgggg ctgggcgatc tggtgcttac ctgtaccgac
     6541 aaccagtcgc gtaaccgccg ttttggcatg atgctcggtc agggcatgga tgtacaaagc
     6601 gcgcaggaga agattggtca ggtggtggaa ggctaccgca atacgaaaga agtccgcgaa
     6661 ctggcgcatc gcttcggcgt tgaaatgcca ataaccgagg aaatttatca agtattatat
     6721 tgcggaaaaa acgcgcgcga ggcagcattg actttactag gtcgtgcacg caaggacgag
     6781 cgcagcagcc actaacccca gggaaccttt gttaccgcta tgacccggcc cgcgcagaac
     6841 gggccggtca ttatctcatc gtgtggagta agcaatgtcg tgtgaagaac tggaaattgt
     6901 ctggaacaat attaaagccg aagccagaac gctggcggac tgtgagccaa tgctggccag
     6961 tttttaccac gcgacgctac tcaagcacga aaaccttggc agtgccctga gctacatgct
     7021 ggcgaacaag ctgtcatcgc caattatgcc tgctattgct atccgtgaag tggttgaaga
     7081 agcctacgcc gctgacccgg aaatgatcgc ctctgcggcc tgtgatattc aggcggtgcg
     7141 tacccgcgac ccggcagtcg ataaatactc aaccccgttg ttatacctga agggttttca
     7201 tgccttgcag gcctatcgca tcggtcactg gttgtggaat caggggcgtc gcgcactggc
     7261 aatctttctg caaaaccagg tttctgtgac gttccaggtc gatattcacc cggcagcaaa
     7321 aattggtcgc ggtatcatgc tcgaccacgc cacgggcatc gtcgttggtg aaacggcggt
     7381 aattgaaaac gacgtatcga ttctgcaatc tgtgacgctt ggcggtacgg gtaaatctgg
     7441 tggtgaccgt cacccgaaaa ttcgtgaagg tgtgatgatt ggcgcgggcg cgaaaattct
     7501 cggcaatatt gaagttggtc gcggcgcgaa gattggcgca ggttccgtgg tgctgcaacc
     7561 ggtgccgccg catactaccg ccgctggtgt tccggctcgt attgtcggta aaccagacag
     7621 cgataagcca tcaatggata tggaccagca tttcaacggt attaaccata catttgagta
     7681 tggggatggg atctgatgtg ctgtgatcgt gccggatgcg atgtaatcat ctatccggca
     7741 tacagtaact aatctctcaa caacgctccc ggatatccca gctgccgcca ggcttcatac
     7801 accactaccg acaccgcatt ggacaggttc atgctgcggc tgtccggcac cattggaatg
     7861 cgaatttttt gttcagcggg cagggcatca agaatgctcg ctggcaggcc gcgtgtttcc
     7921 gggccgaaca tcagataatc gccatcctga tagtttacgg cgctgtgagc aggcgtacct
     7981 ttcgtagtga gggcaaacag gcgctgggga ttttctgctt cgaggaacgc gcgaaagtca
     8041 tgatgacgcg taacggcggt aaactcgtga tagtccagcc ccgcgcggcg caggcgctta
     8101 tcgtcccagg caaatcccat cggttcgatg atatgcagac gaaagccggt attagcgcaa
     8161 agacggatga tattgccagt atttggcgga atttctggtt cgtaaagtac gatgtttagc
     8221 atactgcccc cttagtgcgg gggcaggata gcagaaaatt ggtgagcagt gcggcctttt
     8281 cccctcaccc cggaggggcg agggagaaaa aaagagtgcg gtgtcgtttc agagtgaggg
     8341 agtacataca gcgccgaacg gtcccctctc cctgagggag agggttaggg tgagggggcg
     8401 caaacgacta tgccgcattc cctttcgcca tcggagccag tgccgtaggc aactctttac
     8461 ccagcccctg caccagcgaa tcttgcgtaa tttcactgat cgatttcgcg ccagtcagcg
     8521 tcatcgccac tttcatctct ttttcgatca gatttagcag gttagctaca cccgcctggc
     8581 ccgctgttgc cagcgcatac aggaaagcac gacccagcag tacggtgtcg gcaccgagcg
     8641 caatcatacg cacgacatca agcccgttac gaattccgct atccgccaga atggcgatat
     8701 cacctttcac cgcatctgca atagcgggca acgcacgagc ggaagagagt acaccgtcca
     8761 gctggcggcc accgtggtta gaaaccacaa ttccatcagc accaaaacgt actgcatcgc
     8821 gcgcatcttc cggatcgagg atccctttga tcaccatcgg gccatcccag aaatcgcgga
     8881 tccactcaag gtctttccat gagatggacg gatcgaagtt attccccagc cagccgatgt
     8941 aatcttccag tccggtcggt ttgccgagat aagctgagat attacctaaa tcatgtggac
     9001 gaccgttcag gcccacatcc cacgcccatt gcggatgcgt caccgcctgc aagtagcggc
     9061 gcattgccgc atttgggccg ctcatacctg aatgcgcatc acggtagcgt gcgcccggtg
     9121 tcggcatatc cacggtgaaa accagcgtcg aacaacccgc tgcttttgct cgctccagcg
     9181 cgttacgcat aaagccgcga tcgcgcagta cataaagctg gaaccacatt gggcgcttga
     9241 tggctggcgc gacttcttca atcgggcaaa cggaaaccgt cgagagagta aacggaatgc
     9301 catgcgcgtc cgccgctttg gctgcctgaa cttcgccacg acgcgcatac atgccacaca
     9361 aacccaccgg tgccagtgct accggcatcg acaatttctc attaaacagc gtcgtttcca
     9421 ggcttaagtc ggacatgttt ttcagaatac gctggcgcag tgccacttct gacaaatctt
     9481 ccacgttgcg gcgcagcgtg tattcagaat atgcaccacc atccatatag tggaacagga
     9541 acggcggcag aatgcgttgc gctgcggcgc gataatcgct ggctgcggaa ataatcatgc
     9601 gtttttctcc ctcgaatgct cattatgctc accgggcagg cgggtaatcc gtgcgtggcg
     9661 agcctgatct tcatcgaatc gtttcatggt ggtgtgaaca aaactaaggt gcgccatcat
     9721 tgctttacgc gccccgtcgg catcaccagc aaaaatggcg tcaatgacag cctgatgttg
     9781 ttcggtcagt tgtgaaaaaa ccggtggcac cagatacatc cgctgacggc tatgcttcac
     9841 tgaggattgc aggacatcga agaaaccgcg catggtttgc agcagcacga tgttatgtga
     9901 ggcttcggca atcgccagat gaaaacgaac gtccgcttgt gaggcgatat ccgggtcttc
     9961 acttagcgtt gcttcaaagc aaagctgaat cttttctttg tcgccaggtg tggcgcgcat
    10021 tgccgcatgc catgcggtgc tggcttcaat ggcgtagcgg gcttccagaa tatcgaaact
    10081 gtaatccgga tcatcggcca tcagcgtttt tagcggctgg acgatgtttt gctccgacca
    10141 tgtgtcatga cgccagcgaa taaacgtccc gccgccgcgt cgactgagca gcacgccttc
    10201 gctcaccagt tttgctagcg cctcgcgtag tgaattacgt gatacgccga gttgcatcgc
    10261 cagttggcgc tcagcgggca acttcatgcc cgcttccagg tttttttcat caatcagcgc
    10321 ccgcacacga tcggcaacct cgtctgacag gcgtctgggt aaaacaatca ttaaggaatc
    10381 atccacgtta agacataagc ctgaagcgtg gtgatcacgc ccaccataca ggtgaagatc
    10441 aggctgtgtt tgacagtaaa gcggaacaaa tcagactctt tgcccaccag gcctaccgcc
    10501 gcacaggcga tagcgataga ttgcggggag atcatcttac cggtgacgcc accggtggta
    10561 ttggcggcaa ccagcaacag atcagagacg ccaatttgtt gtgctgcggt ggcttgcagc
    10621 gcggcgaaca gggcgttaga tgaggtatcc gacccggtca ggaatacccc cagccagccg
    10681 aggaacggcg agaagaaggt gaatgcatga ccggtgtgcg ccagtgccag tgccagtgtt
    10741 gatgacagtc cggaatagtt cgagataaag gcgaacgcca gcaccatgcc gatggagtag
    10801 atgggtagag ccagttcttt cagcgtgctg ccgaaggtgc tgatagcgtc agacggtttc
    10861 atcttcagcc agacaatcga gagcagtgca gcaaacagaa tggcggtgcc ggtggcagag
    10921 aaccagtcaa acttaaacac ggcggcatag gctgtagcct cgctgaccac tggtggcata
    10981 cgggcaacca gtttatcgag gtacggcacc ggaatgttga tcacccactc atacagcgca
    11041 ccacccgatg cgaacagggc tttaaacggc gggatgctcc acagtgttac ggtagcggtc
    11101 aggaacagga acggtgtcca ggcacgtaac acctgacccg cggtgtaacc ggtgtgggcc
    11161 agcgtcatat caacctgtga cgcccccaag tcaccgaagc ggaatacgcg aactggttgc
    11221 cagcgtttga ggaacagcgt caggcagagc agtgatacca gcgaagagat aatgtccggc
    11281 agctccggcc caatgaagtt ggagctaagg tactgagcga tggcaaacga gccgcccgca
    11341 accacgaccg caggccacgt ctctttgata ccgcgccagc cgtccataat cgccatgatc
    11401 cagaacagca cgataatggt cataaacggt agctgacgcc ccaccatctg gccaatctca
    11461 aaactgtcga tgcctgttac ttgtccggca accaggattg gaatgcccat cgcgccaaat
    11521 gccactggcg cggtgttaac aatcaggcac agtccggcgg cgtacagcgg tttaaaaccc
    11581 aggccgacca gcaatgcggc ggtaattgct accggtgcgc caaagcctgc ggctccttca
    11641 aggaaagcgc cgaaacagaa accgacgatc agcatttgca gacgctggtc aggggttatc
    11701 gaaagaatag acgagcgaat gatgtcaaac tgcccggttt tcaccgagat cttatagacg
    11761 aacactgctg caataatgat ccacgcgatg ggccacaaac catagaagaa gccataaacc
    11821 accgaggcca gcgcgttagc gaccggcatt ttatagaaca gcaaagccac ggcaagggcg
    11881 attgctaccg tccatgaggc ggcgacgtat cctttcagtt tgagcttaat cagcgcaaag
    11941 aagaaaaaca ggatgggaag cgatgctatc agactggaaa gccagatatt cccggcggga
    12001 tcgtagtttt gttgccagag attcattgca ggtctcctgg agtccacgca catcgtgtaa
    12061 tgagtctgtt gctcatctcc ttgtcacacg ttgtgtaaaa gtggcactgc caattgtgtt
    12121 gtgtagggat aatgacaatg aatggttaac taaatgttat gtattggcaa tattgatgtg
    12181 agttttaatt ggggaattgt gaaccagagg tcagataagc gaagaattgg tagggccaat
    12241 tcttgtgggg gggagatgtc tggtaaagaa aagggcaatc ctcatcagag aattgccctt
    12301 agattgttga cccggagcgc cttatcttgc ctgatgcgcg taacgcatca ggcaatttgg
    12361 cgtttgccct tagtcacact aacttaatgg atcattacca ctgaataccg gcaccgagtg
    12421 cggcggagta ttcaccctgg ctattggtac taccttgtaa tttgtagacc caacgaccat
    12481 tggcgctcac catcgataca cctaaagcaa ctgccgattc accgttgtaa gtaccgccac
    12541 caatagaagc catgctggca cccggtgtat aagcctgcgg cagaccggtc attgccattg
    12601 cagaagcgat accaccgctc aacttgcttt cagttttaga cagtttgtta tccatctcaa
    12661 ccatccgctg atcggtgtat tgcttcgttt cctgcacgct ttgcttcaac tgcgcgtagt
    12721 tcaccgcgtc gttgttgttg acgccagcgg agacgttgct gatacgagtc gtactgccgt
    12781 tgccaccacc gagggtgata ttgctatagt cgatagaacc atcagctttg gtgtcgtagc
    12841 gcacgccgcc tgcttcagaa gatttcagct gcgacacgtt aaccgcatcg gtggcattaa
    12901 caccggcagc tacgttggtg atacggcgtt ggttagtaga agaacctaca gagattgtat
    12961 tttcttcctc tgcaacggaa ccggtaccca gtgcgacgct gttgtcagcg gcagcaatgg
    13021 aaccagaacc aatagcaacg ctatctttac cctgggcgtt ggcatctacg ccatcggtgt
    13081 tggtcttgaa gtacttggtg ctaccggagg tgacaatgtc gccaataccg ttttcgatat
    13141 tagtaacgcg agtatccaga ttagtgacct gattgttcag gttagtaatg ctctgggtat
    13201 tctgggaaac ctgcgcatcc gttactttca actgacccac gttgaccgca tcggtatcag
    13261 ccgaacctgc tgcgacgtta gtgatctgac gttgaccatc cgcactaccg actgagaatt
    13321 caccgacaga gttctgcggt gcgtccatgt tgtaggcggt ataattggtt tgagcgccac
    13381 gagtggtcgt agaaccatta cctatcgcaa tactgttgac atgaatgact tgcgcattgc
    13441 taccaatggc aatgccgtta agtgcattcg cgtccacgta ggcaccataa ccgataccaa
    13501 tacctttatc gccattcacg atggttttcg cacccattgc cagcgagtca gttcccactg
    13561 ccagtgaatc ttcttccgtt gaattagcgt ggaagtattt agtcggcgtg gttgcgaccg
    13621 caccaatcgc attctgcaat tgacgaaccg taacggcgtc atgggcttcg gaaccatcgg
    13681 ctacgttgat gatttgacga tatttaccgt catcaccgat agacaatgca ccgagcagtt
    13741 cgccatccgt ggtgtcgtaa ccaataacaa cgccattttc cgttatgctg gtgtcacggg
    13801 agcctttggc aatcactcgg ctggaaacag agctgctacc cagggcgata cccgtatcaa
    13861 cgtcgctgta gctgccctga ccaatagcta cgctgctatc gcctttggcg acagagttat
    13921 aacctatagc tgtagcgcca acaccctgtg cgctggcgtc gttgaacgct aagccgtcgt
    13981 cgttagtacg cacatagtta atacccgcac cgttttcttg gatataggtt gcgtcggtgt
    14041 taccagcgag ctgattgata atttgggtgt tctgctcaat catcatattc gtcgcattca
    14101 actgagaacc gttcactgca tcggtactgt cctcactaat actgccatta gcgacattag
    14161 tgatgatgct ggctttaccg tcatggctgg cattgtaggc tccagcaccg ccattggcag
    14221 tctcatccca cagcaaggcg ttatcatcaa gcgcatccag ggcgtcaccg acgttgttgt
    14281 actcggcgtt cacaatggtg taagtcggtg cggtgatggt gccgtcagcg ttgacttctg
    14341 cgtcaccacc cagcgcatca gcgatgtact tattggtggt atagagttgt gagccgttaa
    14401 tcgcgtcgct gctggcagca gagattgcac cgttagcgac gttagtgatt acactggcag
    14461 ttttatcttt tccgtgagcg gcgctaaatg caccattttc accggcgtcc gcatcccaga
    14521 gcagagcgtc gtcaagagtg gtatcgatag cattcagggc atcaccgaca ttatcgtaat
    14581 cagcattagc aatggtgtac gtcggcgcag tgatggtgcc gtctgcattg acttcggcac
    14641 caccccccag cgcatcaaca acataactgc tcacgccgtg gagttgtgag ccgtttactg
    14701 cgtcagaact ggagtctgaa atttcaccat ctgcgacatc agtgatcacg cttgcgtcac
    14761 cattagtacc gtgtttggca ctgaatttac ctgcggtggc atcccaaagc agagcatcgc
    14821 cgagagacgt gctaaatgag gagttaatcg cagccagtgc atcgccgacg ttataataat
    14881 ttgtttcacc gattttgtag gttggaccgg taaacacacc gtcagtattc acagaagcat
    14941 tgccgccgaa gtaggtggcg atattgctgc ttaagtcata aagctggcta ccgttaatgg
    15001 catcggaact ggttgcagtg actgtgccgt ccaggatatt ggtgattttg ctggcggtat
    15061 tgttgccatg agctgcagtg aagacaccat tgtccttatc ccatttcagc gcatcctcac
    15121 caagattagt caccgtctca gtcagattag agatgttggt ggtgttagtg gcgatattgg
    15181 tggtattcgt cgccacggca tcgttggtgg ttttcagctg agagccgtta acggcatcgg
    15241 tgctgccagt cgtcaggtcg ccatctttaa cgttggtgat tttgctggtg gtctcggtgc
    15301 catgagctgc cgtgaataca ccattgtcct tatcccattt cagcgcatcc tcaccaagat
    15361 tagtcaccgt ctcagtcagg ttagagatgt tggtggtgtt agtggcaata ttggaagtgt
    15421 tattggcgat attggtggta ttcgtcgcca cagcatcgtt ggtggttttc agctgagagc
    15481 cgttaacagc atcagtgctg tcagccgtca ggtcggcatc tttaacgttg gtgattttgc
    15541 tggtggtatc ctggccgtga gctgcactga atgccttttt agtttcgttc cagagcaggg
    15601 catcagcctg aaggtcacca acggaatccg tcaggttggt gatattggtg gtattggtgg
    15661 caatatttgc cgtattcgtg gcaatgttgc tggtattagt agcgatattg gcggtgttgg
    15721 cttcgacatc gtcattggta gctttcagtt gggaaccgtt aaccgcatcc ttactggagg
    15781 ctgaaatcgt gccatccgca acatcggtga ttacgctggc agttgggcta ctagtaccat
    15841 gagcagcgct gtatttgcct ttggtttggt cccattgcag ggtgttttca tcaagtacag
    15901 cgagcgcagc ccctacgtta tttttgctac cattttttaa attgtaggtt ggtgctgtaa
    15961 cagtaccgtc atcaacatct actgcagccc ctcctccaag cctttttgct accgagtcgc
    16021 taatggcata aagctgtgaa ccattaatgg catcgtaact gtcagacttg atcgcgccat
    16081 ttttcacatt aacgatcttg cgttttaatg aactattgcc gacagagacg gtattgtcta
    16141 aatccgcaat ggaatctgcg cccagcgcga cagagttaat accgttggca ctgctattac
    16201 taccaagcgc aagactctta tcaccagttg cattactggc tgtgcctaag gcaatggtgt
    16261 ttaagcctct cgactggcta ccattaccca gagcgatggc atccacgcca tcagctttgt
    16321 tgccctggcc tatggcgatt gcattatctg ccagagcctg gctgccattt cctaatgcga
    16381 tagtattgac tttatcggca aggcttccga accccattgc catgctatta gtcccttttg
    16441 ccttcgcatt attgccaata gcagttgcat tttcagcggc ttcggtctca gcacctatcg
    16501 caatagcgtt cgctgcgctg gcaagtgatt gtctgccaaa tgcgaggctg tcagtaccgc
    16561 tagctacact gctacgcccc agggcaattg cgttttcttc agatgcgacg ctacttgctc
    16621 ccagcgccat tgaatacgct tttgacgcat tggcggtgtc gccaagagca atactcataa
    16681 tctcagtagc tttagtggca tttcccaggg caatggcgtt tgctccttcc gctttggaag
    16741 agtcccccat agcaatagac aatttaccca atgcttttga ataacggccg agggccattg
    16801 agtattggcc caatgaatac gaagaggcac ccagtgctat acttctatcg ccttctgata
    16861 ttgcactaac cccaaaggcc attgatgcac caccaatcgc atgggttttt gacccgatgg
    16921 cagagctata ttggccttca gctatagcgt cataacccac agcagtactg gaaccactgg
    16981 tgttcataaa agtatttgct ttcgcccctt tgcctatagc aacccagccg tcgccagctg
    17041 atccactacc gtaatcaaca ccctgaccgt tgtcattccc ggcattcgcc aatgccccaa
    17101 acgacgacaa cattccaccc gcaaccagcg cagaaattaa cagcttactg cgcccagatt
    17161 tcttgccacg gctttttgcc gtttcgctgg taacagtata attccctgtc gcagggttcc
    17221 agataacttt aaatattttg ttcattaatg atgctcgcta ttccttgtta taagcgtact
    17281 aactgcattt atttttttgc ttcttttctg ccgattttta gcagaaggaa gattgtcgaa
    17341 ctgaatattt tccatgttga tggctaactt cagatatccc aggttattgg gcgatacacc
    17401 cttcagggtc aaatttcagg gtcaaattaa tgtcttgcca ttgacctggt aataatttca
    17461 atgtggattt cattgatgtg ttgaaaagta attgttgcca ccgggcaaac tctttggcat
    17521 cgacaggttt ttcaccaccc catgtcgcat taaaaccaat atttgagata ggtgtactta
    17581 ataagttgac cagtgaaata tgaataacac tgccattctc tgtggcgtca atggataaca
    17641 ctcgacccgc taaagccacc gaaccattgt tcaggatgaa atacttcgtt tttgctgggt
    17701 taaactcggt aaaaataaaa gtgttttgtt gatctttaac ggatttaatt acgtgagtta
    17761 aagtctttgt actgcttatt atgccagttt gttgtttttg aatagtttcc agttgactct
    17821 ttagctgtgc ttgttgtttt tcaagtagat caattttatt gttatgttct tcttgttgtt
    17881 tcaataaact actttctggc ttgccttctt caatgtagtc acatcccgtt aatgcgcaga
    17941 gaactgaaac agtgacagca ctgaaaaaaa atcttcttca agttcatgta aaaatcctta
    18001 tttcctccga ccagcgatca atgccggtag agttgtccat tgtattattg gtgatctatg
    18061 ttgctttaca ttaatgacag tgatgtgtgt cgattaaata ttgttttaag cattcatcat
    18121 aaaaatgtat cattcgtttc aa
//
LOCUS       DAZGTR010000090        18025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGTR010000090 DAZGTR010000000
VERSION     DAZGTR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.090"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..146
                     /locus_tag="ACHLCY_003392"
     CDS             <1..146
                     /locus_tag="ACHLCY_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0370578.1"
                     /translation="PVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FNW"
     gene            158..355
                     /locus_tag="ACHLCY_003393"
     CDS             158..355
                     /locus_tag="ACHLCY_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0370579.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            440..1282
                     /locus_tag="ACHLCY_003394"
     CDS             440..1282
                     /locus_tag="ACHLCY_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0370580.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPNNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(1563..2099)
                     /gene="yghD"
                     /locus_tag="ACHLCY_003395"
     CDS             complement(1563..2099)
                     /gene="yghD"
                     /locus_tag="ACHLCY_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFP0370581.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVLNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(2101..3279)
                     /gene="gspL"
                     /locus_tag="ACHLCY_003396"
     CDS             complement(2101..3279)
                     /gene="gspL"
                     /locus_tag="ACHLCY_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP0370582.1"
                     /translation="MSSMLEIFFPLCASDPIRWQRHTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDISRKQIPTILPALLEEELNQDIDELHFAPLKINQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(3276..4253)
                     /gene="gspK"
                     /locus_tag="ACHLCY_003397"
     CDS             complement(3276..4253)
                     /gene="gspK"
                     /locus_tag="ACHLCY_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP0370583.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ASFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNETELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(4250..4855)
                     /gene="gspJ"
                     /locus_tag="ACHLCY_003398"
     CDS             complement(4250..4855)
                     /gene="gspJ"
                     /locus_tag="ACHLCY_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP0370584.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(4852..5223)
                     /gene="gspI"
                     /locus_tag="ACHLCY_003399"
     CDS             complement(4852..5223)
                     /gene="gspI"
                     /locus_tag="ACHLCY_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP0370585.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(5220..5783)
                     /gene="gspH"
                     /locus_tag="ACHLCY_003400"
     CDS             complement(5220..5783)
                     /gene="gspH"
                     /locus_tag="ACHLCY_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP0370586.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(5787..6242)
                     /gene="gspG"
                     /locus_tag="ACHLCY_003401"
     CDS             complement(5787..6242)
                     /gene="gspG"
                     /locus_tag="ACHLCY_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP0370587.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDIYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(6259..7482)
                     /gene="gspF"
                     /locus_tag="ACHLCY_003402"
     CDS             complement(6259..7482)
                     /gene="gspF"
                     /locus_tag="ACHLCY_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP0370588.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(7482..8975)
                     /gene="gspE"
                     /locus_tag="ACHLCY_003403"
     CDS             complement(7482..8975)
                     /gene="gspE"
                     /locus_tag="ACHLCY_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP0370589.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVDMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(8975..11035)
                     /gene="gspD"
                     /locus_tag="ACHLCY_003404"
     CDS             complement(8975..11035)
                     /gene="gspD"
                     /locus_tag="ACHLCY_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP0370590.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(11065..12024)
                     /gene="gspC"
                     /locus_tag="ACHLCY_003405"
     CDS             complement(11065..12024)
                     /gene="gspC"
                     /locus_tag="ACHLCY_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP0370591.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(12042..12452)
                     /gene="gspS2"
                     /locus_tag="ACHLCY_003406"
     CDS             complement(12042..12452)
                     /gene="gspS2"
                     /locus_tag="ACHLCY_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFP0370592.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(12518..13327)
                     /gene="pppA"
                     /locus_tag="ACHLCY_003407"
     CDS             complement(12518..13327)
                     /gene="pppA"
                     /locus_tag="ACHLCY_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFP0370593.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGVVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(13475..>18025)
                     /gene="sslE"
                     /locus_tag="ACHLCY_003408"
     CDS             complement(13475..>18025)
                     /gene="sslE"
                     /locus_tag="ACHLCY_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFP0370594.1"
                     /translation="KYKKSLLAAILSATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVK
                     PDPTPNPEPTPEPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDIT
                     CNDESSDGFTFTPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAG
                     SDNKKSNALSLVTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNE
                     EVENNAATDKAPSTHTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRL
                     VDSQGYGVAGVNYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALT
                     ELGDEVRGANIDQLIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATL
                     DEGEQVVNLPNEFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVI
                     NKLWGVDTNYKSVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWL
                     AFGEKRAWDKNELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHY
                     NSILRCPNGYSWNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKASMTVG
                     TNLDTVYFKRHGQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVT
                     QVGNDPYAIPLRADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVR
                     LLDAAGLSMALNKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGE
                     VKWKYQVENKPDDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKEKIFAA
                     FPGLKECTNPAYHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLG
                     TNIQRLYQHELYFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFK
                     TFTEFLNCYANDAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPL
                     TRLMLGRSWWDLNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAP
                     AQKEVTIKSNANVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVP
                     YGGLIYIKGNSSTNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTP
                     KKNLNASNYTGGLEQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRFTND
                     VQISIGDAHSGYPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVA
                     NNVLALYMQDRYLGKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAE
                     KNFDIKKWYPEGELPKFFSDREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNG
                     NAADTLMLCASWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLASLK
                     LPKPEQGPETINKVTEHKMSVE"
BASE COUNT         4041 a         5108 c         4529 g         4347 t
ORIGIN      
        1 acccggttct gcggcttaaa cgcctggggc tgtcaaaaat gttccgccgt ctggttgtca
       61 cacttatccg gcgttatggc gtcagtctca ttcatctgga tgccagtgct gaatgcctgc
      121 cgggtttacc tactttcaac tggtaaccag aaacaacatg aaatcattaa ccactgaaac
      181 agcgctggat atcctcattg cgtggctgca ggacaatatc gactgcgaat cgggaattat
      241 ctttgacaac gatgaggata aaacggattc ggcagcactg ttgccctgta tcgaacaggc
      301 ccgggaggat gtccgtactc ttcgtcatct gcagcttctg caccagaacc ggtaaatatc
      361 actcaccata cttcattcca ctgacgtcag cctgaacagt gctggcgttt tcgtttataa
      421 accaaaaggg aaaccgataa tgtcagacat cacaatctcc cgtccggaag tggtcaccgg
      481 gcatacggac gttatctgct caaccagtat ccgccacatt ctggctgtac gaaagagtac
      541 actactgcaa atcgacacac ttatccggca actggctgaa atatcagcaa tgacagaaag
      601 tattggcggt aaagccgccc cggactgggc catgaaacag gattttcgct gcggctgctg
      661 gctgatggag aaaccggaaa ccgcaatgaa agccatcaca cgcaatctcg atcgcgaaat
      721 ctggcgtgac ctgatgcaac gttcagggat gctttcctta atggatgcac aggcccgtga
      781 tacatggtac cggtcactgg agtacgataa ttttccggaa atcagtgaag cgaacattct
      841 gagcacattt gaacaactac accagaataa ggatgaggtg tttgagcgag gggtgatcaa
      901 cgtcttcagg ggactgaact ggaattacaa aaccaattgc ccctgcaaat ttggcagtaa
      961 aattatcgtc aacaacctgg tgaggtggga ccggtggggg tttcatctta tcaccgggca
     1021 acagactgac cgactcgtcg acctggaaag aatgctacat ctgttcagcg gcaaaccgat
     1081 ccccaacaac cgggaaaaca tcacgattcg tctggatggt cacatccagt ccgttcaggg
     1141 taaagagcgc tatgaagacg agatgttcat cattaaatac tttaagaagg gatctgcaca
     1201 catcactttc aaaaggctgg aactgattga cagaattaac gatataatag ccaggtactt
     1261 tccttctgtg ctctcagcct gatcccgagt gtgattccct ttcgatatca aaagggactg
     1321 cgggtataat tgagggtaca tttttaatta aaccaaacaa attgtattaa tatcaatatg
     1381 ataaaagaaa ttttcgattc cgagtccggc accactaatt cttaagaacc cgcccacaag
     1441 gcggtttttt gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc
     1501 tgggggaatt actctcgcca tgttaacgag agtaatttta ttgatactaa tctcctgata
     1561 ctttaccccc gtccaaactc cagccgctgc acattcacca tcccagtctt ctcagcagca
     1621 ctggcatcaa tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc
     1681 agcaggtcat ttaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct
     1741 atccgcttga tgactaccga gtgcgcggaa gcgctgtcac tgataacccg cgatacctgc
     1801 gcaggcgttg ttgtggcgga ttttcgcgcc gccataatat ccggcgcggc gctcctcagt
     1861 ctggtgttca tcgccaccag ttgctgcaac attgtctcct gttgttctat ccgttcgctc
     1921 aacggctgcc agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt
     1981 aacatgccct tttcacgcgg agaacgcccc gccaggtgtt gtgccagcca gtgttcgcca
     2041 cggcttaact ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg
     2101 ttatttcctc cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaatgcgtc
     2161 ctgttgcaca acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt
     2221 agcagcccgc aactggaggt gaagcgtctg gcgtttttga tcaaaggtga aaccacgcat
     2281 ttcgatgtcg gaaagtgacg ctgatttcag ggtgctggct atcgctgaca actcggcgag
     2341 cagccgggta tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg
     2401 taaattcaca atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt
     2461 gcggctttgc gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag
     2521 cgcaaccaga atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg
     2581 gtagctgacg cgaggctgcc acggccctgt cagcaggttc ccttccggtt cgccataagt
     2641 ggtaatggcg ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga
     2701 tagcttttcc ggtgcaatgc cgactacggt tagcgaaagc ggtaaattct gctcattgag
     2761 ctgggcgcgg aacatgaccg gagccagcgc ccgcccggcg ctccatcccc ggcattcatc
     2821 gatgcggcag ataacccgtt gtgcatcgcc agccataaac ccacaaggaa tggacatcca
     2881 gtccggcgcg acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg
     2941 catatgctgt tggtgaatca cggctacggt tgccagttgc tgattgattt tcaacggggc
     3001 gaaatgcagt tcgtcaatat cctgattcag ctcttcttcc agtaaggcag gcagaatagt
     3061 cggtatctgc ttgcgggaga tatcaggcag ttcaacctgc cagacgctga tccattcgcc
     3121 gggaatgtag agtcgaatcg catcagtttg cagccattgc tgaagctgtt cgtcagcaac
     3181 gtcaggccag ataccgtgct ccacgtcggg tgtatggcgc tgccagcgga tggggtcgga
     3241 ggcgcaaagc gggaaaaaaa tctcaagcat ggaactcact cactttctcc tgtctgatgc
     3301 cagagaaccg aaaagtgttg tgggcccatg cggacaatta acgagttcat cgtcagttca
     3361 gtctcattca cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg
     3421 gtttttagct gttttttagt acgctcatcg acgtcagcga gtagcggctg tgcaagaaac
     3481 tgatcgacat cttcccagcc cttcgccgga cgttgttgta ataacgcccg cgcctgaaca
     3541 gggcttaacc acgggtcaaa cagcgcctca agaatcacac tttgcgtgac gtctaaagta
     3601 ttgatgttga tttgctggcg ggtcatcggc agcgcacaga ccagcggttt cagtttttga
     3661 taaagcccgg cgtccattcc ctgcaccacg cgcatctcgc tgatatcggc cagcggttga
     3721 ttggcggcgt agaacggcac cgaacgggcg agatactcgc tgtcttcacg gcccaggcgc
     3781 gtctgcacgc tgcggtcctc gtcaataaac tcccacaggc tttcggctat cagttcggcc
     3841 cgataagcag gcacatcgag gcgcgagatc agggcaatca gttgttgtac cgcgagcgga
     3901 cgcgacgccg tcgtcggctg agcgagggca ttcaggttaa agctggcctg tgcgtcacgc
     3961 agagtgacgg cgatttgccc ctgcggcagt gggaaaaaac gagggccgga agcccacggt
     4021 tgtgccagat gcacgcgctt ttcatttttc aggctcagac tgagcgcgct cagcgccagg
     4081 ctttctgcac tggcgctgta ccacagcgcc tgctggtact cctgctggct gcgcgttcgc
     4141 ccaagttgct gctgcatccg tccggaaagc gtgatggtta ccagcatcat aaccgccagc
     4201 aataccagca ccacgaccag tgccattccg cgttttggtg gtgaggtgat catgataatt
     4261 gcggcccgcg taacaaccag atgcgttcaa tctcgcccca ttgcggcgaa tgcagagtca
     4321 tgcgcactgc cacagggatc gcctgtactg acgaccagct ctcctgccag cgcgtaccat
     4381 catagaactg caaacggagc gaatccgccg gaatcaactt ttgcattgtc ggcttcacgc
     4441 tgcctgcggc atcggtcagc ggccaggcta accgttcgag ataaccgtca tgaatgcggt
     4501 aaccgacggt gagcagattg ctgcgtggca gacgcatcaa cgggttaacc acgccgccgc
     4561 gcacaaagcg catgccttca ctctcagacg ccagcacgcc agcgcccgcc agtaacgctg
     4621 gttcacgctg gccctgttcg cctcttaccg gacgcggcat catttgtgtc agatcgtggg
     4681 tcagaaaact catcgtttgc tgcatgaggt ttagtttttg atcatgtccg gcgacggcgt
     4741 tattcacgcg tgtgacaccg tttgtcacct gctgcgccat cagtgccagc gaggcaaaaa
     4801 tggcaattgc caccaacatt tccagtaacg tgaaaccagc gcgagtcctt ctcactgttg
     4861 gcctcccacg gcgctaaacc aggcacgtcg tgactgaatc accgacgaaa agtcttcgtg
     4921 acggctgact tcaatatcca ccgcctggag cagcgcgttg cctgtattca atgggattgt
     4981 tcgccagtac cagcggcttc ctgccatatc gctctcgccc tgctgcctga ccgcccgctg
     5041 ctcgcgggtc atcaggtaga gcagcgcggt ctggttttca gccacccagc ccgctaccgt
     5101 tttttcctca agaatttgct gattactcag cgcaccgctg gcaatctgta acaccgccgt
     5161 ggcagccagc gcaaaaatcg ccagcgcgag catcacttcc agcaaggtaa atccgcgctt
     5221 catggcatcc tctcatcaca ttgattgagg gataacgcgc catcgtgcgc cagttttacc
     5281 gcccaacatg cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt ggcgggttca
     5341 aaaggcgaaa aacggatctg cggcgtcttc tttttcgcct ctttttgcag ctccagttca
     5401 atatcgtgca gcgtcaggcg acgacgttgc agctccagcg catactcctt ctgccagata
     5461 tcacttccgg gttgtaacag catctgcacc tgttttggca ccgtaacctg tgccgataac
     5521 cgggtcgcgg aaacgggcaa ccactgcccc tgacgacgct gcataaactg atagccaggc
     5581 gcgtcgatta gcacaccgag tgtctgccct tcaatcactg ccctgtcctt aaactgcgca
     5641 aagcgagtca gaaaatcctg tgccgctttt ttcgcgggcg attcagaatc ggtcgcaaaa
     5701 gtctgtacca cgcccgcact ggcaaggccg ataagaaaaa tcaccagcat gatttccaga
     5761 agtgtgaatc cgcgttcagg cactgcttac tgaaactcct gcaaattcca gttaccgata
     5821 tctgcgccag cgccttcccc gttttcctga ccatccgccc ccagggtata aatatcaaac
     5881 agcccttttt cacccggact gagatactga taatcattgc cccacggatc ctttggcagt
     5941 cgtttaatgt atccaccagt acggtagttg cgggagtccg ccatattggc tggctgctgg
     6001 atcagcgcct caagcccctg ttctgtggtc ggataacgcc cgttatccag tcggtacata
     6061 tccagtgcgt tttccagcgc cacgatatcg ctgatggctt tttgccgatc ggctttctct
     6121 ttgttgccca acaggttagg caccaccaga cttgccagga cgccaagaat aacaatcacc
     6181 accatcactt ccagcagggt aaaacctgcc cgtggttttt gtgtgcggga taacgaattc
     6241 atgtgataac tccgtaaatt acattccaac catattattc agttgcagca tcggctcgag
     6301 gatggcgatg acaataaaca gcaccacgcc cgccatcatc accaccagcg caggctcaaa
     6361 cagccccaac gccagcccca cctgggtatc aaattcccgt tcctgattaa cagcggcctg
     6421 ctcaagcatg gtttccagtt cgccgctctg ttcgccggag gcgatcatgt acagcatcat
     6481 cggggggaac aggcgcaact ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg
     6541 atctgccgcc agcagtagtt gttgttcgac atagcgattt gccgacacag cggcagcggt
     6601 ctgaatacct tccagtagcg gaacactgct ggcggtgagg atgcttaagg tgcgggaaaa
     6661 acgcgccgta ttcagcccgc gcgcaacacg ccctgtcacg ggcagacgca gcacggtttt
     6721 atcccagcgc aggcgcatag taggattttt aagtagccgt tgccccagca ccagaagcgc
     6781 cagcaatccc gccagccagt aaacgccgct ggcctgtaac gcgtcgctca tggcaataag
     6841 cgtgcgggtt gaggcgggta gcgcgtgtcc gagatgatca aactgttcga tgattttcgg
     6901 caccactgcc gtcagcaaaa tagtgactac gcccgttgcc accaccagca gaaccagcgg
     6961 atagagcatg gcctgcaata gacgtgattt caggcgctgt cgctgttcgg tgtaatctgc
     7021 caggcgatta agcaccacgt cgagatgccc ggatttttct ccggcagcaa ccatcgaaca
     7081 aaacagagaa tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg tgtaaccttc
     7141 ctgaatccgg ctgcgcagcg ccattccgag gctttttaca tgcagttttt cactttgctc
     7201 actgaccgcc tgtaagcagg tttccagcgg cattgctgcc tgtaccagcg ttgccagttg
     7261 gcgcgtgaac agcgcaagat ctgccgccgc cacgcgacga tgtgcgtgcc gccgacgctg
     7321 caacatcccc cctgacgaag tattcatccg ggcttcaata tgcacgggga taagctcttt
     7381 accgcgcaac aactggcggg catgacgcgc ggaatccgcc tcaatcatac ctttggtttt
     7441 gcgaccatta cgctccagcg cctgatagta aaacagtgcc attacgcctc catggttacc
     7501 cgcagaactt catcgagaga ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg
     7561 atacccgcag agtgttgtcg gacataacgt tccagctcca gctccccggc ctgacggtgg
     7621 atcaaatcac gcaatgtggc atccaccacg atcagctcat ggatggcagt ccgtccgcga
     7681 aaacctttgt gattacaggc gggacagccc tgtggatggt acagagtgac ggtacgggcg
     7741 tcggtaattc ccagcaggcg tttttcttcg tcagtggctg gtgcgggctg gcggcagtcg
     7801 ggacacagcg tgcgaaccag tcgctgcgcc atcacgcccg tcagactgga agagagcagg
     7861 aaaggctcca cgcccatatc ctgcaaacgt gtgatcgccc ccaccgctgt gttggtatgc
     7921 agcgtggaaa ggaccaggtg tccggtaagt gaagcctgga cggcgatttc tgcggtttcg
     7981 gtatcgcgaa tttcaccgac catcaccaca tccgggtcct gacgcaaaat cgcgcgcagc
     8041 ccacgggcaa aggtcatgtc gacgcgggtg ttaacctgcg tctgaccgat cccttcaatc
     8101 atgtattcga tgggatcttc aaccgtgaga atgttgcgcg aatggttgtt cagctcctgc
     8161 aatccggcgt acagcgtggt gcttttgccg gaacccgtcg gccccgtcac cagaaagatg
     8221 ccgtgcggtt tgtgtaacag ctggcgcaac tgcgcggtca gttgctggct aagccccaga
     8281 cgctccagcg tcaggcgggc ctggtttttg tccagcagtc gcagcaccac gcgctcgccc
     8341 caggcagaag gcatggtgga gacacgcacg tcaatcgccc ggccgcccag caacagcgca
     8401 atacgtccat cctgcggcac gcgcttttcg gcaatgtcca gccgcgccat caccttgata
     8461 cgcgacacca gcagcgaggc cagtttgcgc cccggacgca gcatttcatg taatgtgccg
     8521 tcaacacgaa aacggatcac cagactcttt tcaaacgtct caatgtggat atctgaagcg
     8581 ccttctttga ttgcctctgc cagcatggcg ttgatcagtt tgatgatcgg cgcatcgtcg
     8641 tcgctttcca gcagatcttc cgtttccggc agttcttccg cgagggtaaa aaagtcctcg
     8701 gcagaaccga gatcttccat cagttggcga gcctcggaag agtcccgctg ccagaccgca
     8761 ttcagccgct gttcaaattc ggcttcgtcg atttgccgca gcgtaaaggg cgcgttcagc
     8821 ccccgctgca gctcctgcaa aacagagagc gacaacggat gaacatggag gatctccagc
     8881 gacgcttcgc accatgccac caggctaaac cgacggctga aactgtaggg cagacgcacg
     8941 gtgttagcgg tggtttcctg tgctacaggc accattaacg cgttctcccg gcattaagga
     9001 acgcacgaac ttccggcggt agagcctgat tttgcgctgg cagtaccggc tgcgcggtgt
     9061 gcggcatcag gcttaaacct tgctcatcac gatagatctg ttcggcgcgc atatagttat
     9121 atttgcgctg cgacacgccg tctgccgcca taccgtcacg cagaatggtc ggacggataa
     9181 acaccatcag gttacgtttt tcttttttat ccgccgtcga tttaaacagg ttaccaatca
     9241 atgggatatc gcccagcagc ggcactttcg ccacgctttc tcccgcctga tcgtccatca
     9301 gaccaccaag cacaatcagc tcaccatcgt tagccagcac ggtggttttc agtttgcgct
     9361 caccaaacac cacgtcgagg ctggtctgtc cttccacctt cgagacttcc tgctcaatca
     9421 ccatctgtac cgcgttacct tcgttaatct gcggcgtgac tttcagcatg atgccgactt
     9481 ttttcctttc tactgtattg aaaggattgc tgttattgga gccaacggta gagccggtta
     9541 ataccggaac gtcctggccc accatgaaga aggcttcctg gttgtccagc gtagtgatgc
     9601 tcggcgtgga gagaacgttc gagctggagt cgtttttaac cgcctgcacc agtgccatcc
     9661 agtcgccttt caccacgcca accgccgtac cgctaaatcc agaaagaagc tgggcgagtg
     9721 tggagagatc gccgttggta tccggattaa tggtggtagc gccgttttca ctgatcaccg
     9781 tcgagccttt ctgcggtttc gcctgagaaa tggctgcccc cagcgtaccg atagggatct
     9841 gcgtaccatt agcaaactgc attaatccgg catctttcga cgcccactgc acgccgaagt
     9901 tgatattgct accttcggca acttccacga tcaacgcctc gacgtgcacc tgagcacggc
     9961 gaatatccag ttgttcaatc acgctttgca gcgactgcat gatgtcctgc ggtgcggtta
    10021 caatcagggc attactgtgt ttgctggcgg cgatggatac aatctcacgc ccgctaccga
    10081 ccgtgccttc cgcctcttct ttagccgccg tgagcgtgcc gctgacctgc ttcagcacat
    10141 cgaccagatc ttcggctttg ctgtatttga gatagaaaac ctggctgttg ccgctgcgtt
    10201 ccatttctga gtccagccga cggatcaggc ggcgcatttt gtcccgcgtg gccgggtcac
    10261 cactgacaat cacactgttg gtgcgttcgt cggcgacaat ttgagatttc agcgtcgctg
    10321 gctggttctc gccgctgttt ttcgtcaggc tttccagcac gcgggcgatt tccgaagccg
    10381 aggcgttatc cagcgggatc acctcttcgg tgcgattacc cgcatgatcc acacgctgga
    10441 tcacttccgt cagccgctcc acgacggagg cgcgcccggt gagcataatc acgttggagg
    10501 gatcgtaatt aacaacattg cctgagcctg cgctgtcgat catctggcgc agaatcggtg
    10561 ccagctcgcg taccgacaca ttacgtaccg gcactacttt ggtgaccatt tcatcgcccg
    10621 cgtagttgtc gctgccttca ccgaccagcg gcagcggctc gactttcgca gcgctggatt
    10681 tcaccacctt cagcacgtcg ttttccatcg gtacgacggc atacccctgt gcttccagca
    10741 ggttaaggaa tagctggtaa tactggcgtt cattgagcgg ggtcatggtg cgaatactca
    10801 ctttcccctg tacgcccggc cccataatga tggttttatt aaggttagcg ccgacggttt
    10861 cgatgaacga tttcaggtcg gtatctttaa aattagcggt gaaagtggct tcttccgccc
    10921 agaccggtga actgcataat gccgctgcca gcaccagcgg cagtaaacgc ttttttgttt
    10981 tgaggccagt tgtcttctta cgccagaccg acaacgtaat atcacgccaa aacacgatga
    11041 atgattctcc tggattaaat gcggttagcg cagcgcgatg gaaatgtcgt ggcgcgcacc
    11101 cttgcgtaaa accgtaagtt gaatggaatc cattgaaggt aactgccgca tcagagcaat
    11161 cattgctcgt ggatcagtga aatcctgctg atttagcgca atggcgatat cgccttcctt
    11221 gaaaccgctg gcatcgaaca gagaacgatc tgcccccggc ttcactgcat aaccgacaat
    11281 tccctcctta cgcacaggcg taagctggat atagttaaaa attttctgcg gatctttcgc
    11341 cagtgcctga cgcacggcag tcggtatctc aactggcgca ctgacagcag gttcagcaac
    11401 agcttgcttt gcttcgtcac tgacagcttt tttgttggtc gcggcaacgg tggaacgctc
    11461 ctcttcagcc aggctcaggc gctctatttt tccctgatag cgcagcatca catggtcgcg
    11521 attgatttcc tcaatcaccg cattgtgcga gccaagcgtt tcaccctgca aatagacctg
    11581 ctgtttaccg ccttcttcaa taaccgcgcc aggtctggca ccaaaggcaa tcccgcgcag
    11641 caccacatta agacgcgttt ctgccacagg cacaggttcg ggttgtttta ccggcgtggc
    11701 gacgggctga tatttgccaa accagttttg ctggctgatt aattgcacgt catttttatc
    11761 gaacgttttt gcatccgcac ggggcggttt attcgccgat ggggaaacag ccgtatattc
    11821 cgcagaaaag gagaaatagc gccagagtga atgcgccact tttgcagaaa taataagcag
    11881 catcagccag aacatcccgc gtacaatttt tcgcagatgc tctttgtctg tattaaaaga
    11941 ttgtctctgg ttaagagtgt gacgaatttt tgtcagccat tgaatgagat aaattcgtgc
    12001 gtcacgaaaa acaacccgcg ccaatttata tctacccgac gttatgcttt gactattcca
    12061 caggtggtac gatccagttt ccgctgatac tggttacctg tacgtgtatc attaatcgtt
    12121 atgctgtaat taattccctc ggtaatcatt aatttcaccg ttgggtcagc gcacatttgt
    12181 cgttgatagc tggttaaaaa ggcgtcaggc gtctgcgtgg tctgagtccc cgcttcgctg
    12241 ataatggtca tttttaccgt cgtgccgcta ctttgtgcca gcactaaggt atatcccgca
    12301 gatttaatcg gcaggttttg gctgatattt tgcgcctgtt ttttcgccag taaactggca
    12361 ttttcattat ggctggcaca gccacttaat aatcctgtaa cgctcaacaa tagcgatatt
    12421 aataccctcc ctggcatttg ttttatcgac atggtaaata atctctaagg ttattaataa
    12481 gagttaaaat gtcactttga taatgacgtt gttatcatta aaacaatgcc tgtagataaa
    12541 ttgttgctat accgcccaga cttaaacacg gtccaaaagg cagtgtggtt gatcctcttt
    12601 ttgtaataac ggcatatatc aggccgcagc atgatgcgat taaagcaaca ttgggtagcg
    12661 acaacggccc cacccaacta cctaacgcag cgaacaataa tacatcgccc atgcctaatg
    12721 cttctttacg cagaactact ccggctatcc agcgcaggga gtaaaaagcg ataaacccca
    12781 ccaggacgcc ggtaactgca tcttgtagcg tgagcgggct ctgctgcgcc catgccgcaa
    12841 tcagtcccgt ccacaatacg ccctgagtaa aaacatcggg cagccattgg tgatcgaggt
    12901 caatgacgct cgcggcaatc agccaggcgg ataatatcat caccgccagc gcccatccac
    12961 tttccggcca gaccagactc gccagcaaaa aagcgagtgc cgtcaataac tccaccagcg
    13021 gataacgctt gctgattttc gcctgacaat cacggcagcg ccctttgagc atcagccagg
    13081 agagcagcgg aatattgtca cgtatccgga tggtctgctg acaatgtggg cagtgggaac
    13141 gcggtagcgc aaggcttatt tttgactgcg cactcggcat ttcaccatga aactccgcca
    13201 tttgttggcg cagcatgatg gggtaacgcc aaatcaccac attcaaaaaa ctgccgatga
    13261 tcaatcctcc gacggttgcc agtatgggca tcgccgcggg gtattgctga aaaacatcaa
    13321 aaagcatggt taaaggttgt ttgttgtaac ttgccggatg cgggcctgca agtgtatgcc
    13381 atacggcttt cctccaggcc cgataagctg gcgcaacccg gtgcgcctta tttcatgccg
    13441 gatgcggcgt gaacgcctta tccggcatac aggattactc gacagacatc ttatgctcgg
    13501 taaccttgtt aatggtttcc ggtccctgtt ccggtttcgg cagcttgagt gacgccagcg
    13561 tgttgtacgc cgactggctc acaccgccct cgaagctcat ctcgctcgcc cccggcagct
    13621 ggtaagcatt cgcacccgga ttccatttct taaagaactc cgaaagatcc gtctgggcga
    13681 cccaggaggc acacagcatc agcgtgtccg cagcgttacc gttggattca gcacagtaat
    13741 tcttgccacc aaaggtcttg tcaccaacgt catcgccgcg cgctttacgg tgcatcaact
    13801 ggaacaggtt ccagcctttc atcccttcac gatcgctgaa gaacttcggc aattcacctt
    13861 ctggatacca tttcttgata tcaaagtttt tctctgccca ttccttcagc tgcgcgtaca
    13921 tcagcagacg gtcacccgca ccgccgcgcg cccatgcctg accgttgctc tcctccagat
    13981 attccggtgc gacggtaata tcgtcagcga cacggttcat cttgccgaga taacgatcct
    14041 gcatgtacag cgccagcacg ttgttcgcca cttcggtcgc gcctggaaca gtcagcggcg
    14101 tttctgcggc gttgtgccct acttcgtgcc agatcagcca gtcgttcagc ggcgtcgtcg
    14161 gcagcgtggt gctgttcgtc gagaagctgc tgttcattac cggataaccc gagtgcgcat
    14221 caccgatgga gatctgcaca tcgtttgtga aacgatgttt atgtcccgtc aatgctttat
    14281 aggtaaacat ccggtgctta ccgctcgttt catcacgacc gtagaagtca ttcatcgagc
    14341 tggcaaaggt gtccagatcg ttagcgaatt gctccagtcc gccagtgtaa ttgctggcat
    14401 tcaggttctt cttcggtgtg gtatagacga aagcgtctga ttccagctca cccagcggag
    14461 ccggtgagtt cagatcgttt ttccatgcgc cgtctttata gaacggtgct tttaccacgc
    14521 cagtaaaggt gaagctggca gattcattgg tagagctatt gcccttgata taaatcaggc
    14581 caccgtaagg caccttgaac ttcaccgtac cgctagcgtc cagagagtac gttttagtca
    14641 ctcttggcgg acggttcagc gcaacttcat gcttctcacg tccggtcagg tcgtcagcca
    14701 gcgccacggt gacggtcaca ggaacgttcg cattggactt aatggtgacc tctttctgag
    14761 ccggtgccca caggccagtt gactgcatgt tacctgcaaa ccatttggtc ggattcgagt
    14821 acaggctgat ggtttcagta acgttctgtc cctcttccga tactgctccc ggatacttct
    14881 cgacatcaac tttgatgttc agatcccacc aggaacggcc cagcatcagg cgtgtcagcg
    14941 gtttttccat atagttgagc gggtagctcg ggttcatcat gcccgctttg ctgctaccgt
    15001 caccgtagat catgttgtta tcgaccagcg attttttcag atctgcagaa cacttggtgc
    15061 cgcctgcata ggcatcattg gcgtagcagt tcaggaactc ggtgaacgtt ttaaagccca
    15121 gctcgtcatt tttgccttct tcataacgat agctcgtatc gttccacagc cagaccgaca
    15181 tgttctggta cagacgttcc agatcgacgc tgctcagacg ctcacctttg cgaccattgg
    15241 tccggaagta gagctcatgc tgatacagac gctgaatgtt ggtgcctaaa tccgcagcct
    15301 gcaccatcgc ttttgcagtg tcggcgttaa ggcttagttg cgtatactgt ggaacataca
    15361 tgccaccagt aaccggaacc cccgtgccag gacgatattc caggcagttg acctcatagt
    15421 gatatgccgg attagtacac tctttcagcc ccgggaacgc ggcgaaaatt ttctccttcg
    15481 cagccttcag agaatcctct gttttatgat cggcctcatc aataaaggca taacgcgttt
    15541 cctgtttgcc atctacatct tccagccagc tggcaacttc cagcttcggt ttgtcatcag
    15601 gtttgttttc tacctgatat ttccacttaa cttcccctgt cttactatcg atggtgtacg
    15661 gcagcgcacc atctacggca ggataacgtt catagaccca aatgcccgtt gcgcgctgct
    15721 gacgaacgcg gttcggatac ccttgcggat cgttatttac taccgacttg ttcagtgcca
    15781 tcgacagacc tgcggcatcc aacagacgca caaaaccaga cgcgctctct tccttaagat
    15841 tgctcatcac gttttccatg atcagcaccg atccaccttt gttcagatag gcgatcagat
    15901 cggtcacatc ctgctgagtc agcttcggtt tgctggtatc tgcacgcagc gggattgcat
    15961 aaggatcgtt acccacctga gtcacatatt caaagccgtt aaggatcagc agcggcattt
    16021 cctgcggatc gagatcgcca tagctactta aatgctcaac agagatgccc gcaaaatccg
    16081 gatggaagtc gaacgcagcg ctgtttcctg taacctgacc atgacgttta aaatagacag
    16141 tatccaggtt ggtgcctacg gtcatgctgg ctttcgcgtc cggcgtccat ttatcatcgg
    16201 acagatagcg cagcacgttc tccatgaagt tcttcatgtc atccgggtcg ctgttgagcg
    16261 tacactgccc atctttatta acgcccccgt tccagctgta accgttcggg caacgcaaaa
    16321 tgctgttgta gtgtgggttt ccgataacca tcagtttgcc ctcaccgact tgccccagcg
    16381 aaataaacgg caggttgaaa gtggcggtat cgcgcgtaac gttttccggc tcaacaagag
    16441 aaggcgcttc cgtaatgtac gccagctcgt ttttatccca ggcgcgtttt tcgccaaacg
    16501 ccagccagta gtttttatca ttacgcgcca tcagaatcgg gaaggccgcg ttggagatat
    16561 tcaccaccgc ctgaccgcgc gcattacccg tgctgccata gaagttagta gagtcatgga
    16621 atacatggaa cttgctgaca gatttgtagt tcgtatccac gccccacagc ttgttgataa
    16681 cgccctgaat ctggccgtca ttaacattgc gcgtcgtcag cgagaaccag cgagcctcgt
    16741 tacaaccgtc ggttttcgca caaatcgcgg tatcgatctc tttggcctga cccgtcttaa
    16801 actgctcgat aaattcgttt ggcagattaa caacttgctc accttcatcc agcgtcgcac
    16861 cgttggataa cgagagattg ataatctcgt taatcacgtt gggatattcg gcaaagacct
    16921 tgcgtacatc atccggaaca acacgggtat tattttgccc ggtcgtcgaa tagcgatgaa
    16981 taagctgatc gatattcgcc ccgcgaactt catcacccag ttcagtcagc gcaatggtcg
    17041 acttattgcc gcgcactgaa cccagttcaa aggtatcgat accaaaggag atggtttcgc
    17101 cccagctaaa agaaaattca ccattttccc ctgtcacgcc acggcctgaa ttggtgtagt
    17161 agttgacgcc agcaacacca tatccctggc tatccaccag gcgaccttct gagagaatga
    17221 tttccgaagg ttgatactga taaaactgtt ccgcgttagc cgacacgaag gaggcgttca
    17281 gatccggttt tgttcccgga gtcgtgacgg ggactaccgg tgaagtatga gtggatggcg
    17341 ctttatcagt cgcggcattg ttttccacct cttcattgac cagctttttg aactcttccg
    17401 gtgccagatc gatttgctta tacagcgagt cgaaacgttt actctcgatc accgaggaga
    17461 actccaggca cacctgttct gtattcgccg ggcaactgtt catggaggtg accagcgaga
    17521 gcgcattgct tttcttgttg tcggaacccg ccagttcttg cgcgtcctca agactaaacg
    17581 acactttttc aaccgcacgc aggctacgcg cagcttctga ctgggtgtcg aaggtagcaa
    17641 ttgtcgtgtt gttccctgcc acacaggtga ctttgtcgcc tggtgtaaag gtaaagccat
    17701 cgctggattc atcattacag gtgatatcac cagttacccg caggcttccg cccagggtca
    17761 gataacccgt tttcgtagga acaggttctg gctccggttc tggtgttggc tcaggatcag
    17821 gtgtcggttc cggcgtaggt tctgggtccg gcgttggctc aggcgtcggc tccgggtttg
    17881 gtgttggatc aggtttcact tccggcaaag accctgttcc agaatctaca ggcggcgtat
    17941 cggaggaaga tccggagcca ccgccatcac aaccggctaa cagggttgca ctcaaaatag
    18001 ccgctaaaag cgatttctta tattt
//
LOCUS       DAZGTR010000091        17957 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGTR010000091 DAZGTR010000000
VERSION     DAZGTR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.091"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            135..986
                     /locus_tag="ACHLCY_003409"
     CDS             135..986
                     /locus_tag="ACHLCY_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP0370595.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKAHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESCFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSLPLTLN
                     VKIAELSMNVADLTQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENSHE"
     gene            979..1350
                     /locus_tag="ACHLCY_003410"
     CDS             979..1350
                     /locus_tag="ACHLCY_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP0370596.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1347..2099
                     /gene="fliP"
                     /locus_tag="ACHLCY_003411"
     CDS             1347..2099
                     /gene="fliP"
                     /locus_tag="ACHLCY_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538309.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP0370597.1"
                     /translation="MKRRTQLTLGLGLLALAPVALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTML
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GHAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2102..2374
                     /locus_tag="ACHLCY_003412"
     CDS             2102..2374
                     /locus_tag="ACHLCY_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HFP0370598.1"
                     /translation="MLTIDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGVCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2376..3164
                     /locus_tag="ACHLCY_003413"
     CDS             2376..3164
                     /locus_tag="ACHLCY_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0370599.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYVPVLDNSALTVRVRI
                     ILSLALALAIIITPLIPHPVPHDLISLNSLILTMEQILWGMLFGLMFQFLFLALQLAG
                     QILSFNMGMSMAVMNDPSSGASTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYW
                     PIGNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSL
                     GFPINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3154..4293
                     /gene="flhB"
                     /locus_tag="ACHLCY_003414"
     CDS             3154..4293
                     /gene="flhB"
                     /locus_tag="ACHLCY_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HFP0370600.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPEVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFASFLGLQALPFKLAMNEGLSLYACVMRNFVILFIFFALIDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVAAENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4277..6370
                     /gene="flhA"
                     /locus_tag="ACHLCY_003415"
     CDS             4277..6370
                     /gene="flhA"
                     /locus_tag="ACHLCY_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP0370601.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGADAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RHQLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLASMAPRLAEDLSAVLNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNLVNQAQQGGKVMLDS
                     VPVDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            6475..6753
                     /locus_tag="ACHLCY_003416"
     CDS             6475..6753
                     /locus_tag="ACHLCY_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP0370602.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            6746..7102
                     /locus_tag="ACHLCY_003417"
     CDS             6746..7102
                     /locus_tag="ACHLCY_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0370603.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(7159..7932)
                     /gene="yafL"
                     /locus_tag="ACHLCY_003418"
     CDS             complement(7159..7932)
                     /gene="yafL"
                     /locus_tag="ACHLCY_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0370604.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSQLAEPFWQEHFLGARRILTEETIL"
     gene            8118..8378
                     /gene="dinJ"
                     /locus_tag="ACHLCY_003419"
     CDS             8118..8378
                     /gene="dinJ"
                     /locus_tag="ACHLCY_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP0370605.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            8381..8659
                     /gene="yafQ"
                     /locus_tag="ACHLCY_003420"
     CDS             8381..8659
                     /gene="yafQ"
                     /locus_tag="ACHLCY_003420"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFP0370606.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            8815..9555
                     /gene="dpaA"
                     /locus_tag="ACHLCY_003421"
     CDS             8815..9555
                     /gene="dpaA"
                     /locus_tag="ACHLCY_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP0370607.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(9526..10293)
                     /gene="yafJ"
                     /locus_tag="ACHLCY_003422"
     CDS             complement(9526..10293)
                     /gene="yafJ"
                     /locus_tag="ACHLCY_003422"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP0370608.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(10499..11077)
                     /gene="lpcA"
                     /locus_tag="ACHLCY_003423"
     CDS             complement(10499..11077)
                     /gene="lpcA"
                     /locus_tag="ACHLCY_003423"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP0370609.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            11317..13761
                     /gene="fadE"
                     /locus_tag="ACHLCY_003424"
     CDS             11317..13761
                     /gene="fadE"
                     /locus_tag="ACHLCY_003424"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP0370610.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTASLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(13804..14250)
                     /gene="ivy"
                     /locus_tag="ACHLCY_003425"
     CDS             complement(13804..14250)
                     /gene="ivy"
                     /locus_tag="ACHLCY_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP0370611.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            14431..15198
                     /gene="yafV"
                     /locus_tag="ACHLCY_003426"
     CDS             14431..15198
                     /gene="yafV"
                     /locus_tag="ACHLCY_003426"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP0370612.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(15422..16558)
                     /locus_tag="ACHLCY_003427"
                     /pseudo
     CDS             complement(15422..16558)
                     /locus_tag="ACHLCY_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(16661..17787)
                     /locus_tag="ACHLCY_003428"
                     /pseudo
     CDS             complement(16661..17787)
                     /locus_tag="ACHLCY_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            17869..>17957
                     /locus_tag="ACHLCY_003429"
     CDS             17869..>17957
                     /locus_tag="ACHLCY_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370613.1"
                     /translation="MIYITIFMILPCPVPCSHVFLYVFYIFLFL"
BASE COUNT         4266 a         4525 c         4564 g         4602 t
ORIGIN      
        1 ataatttaat tattttttca ccctattaat aatttgcccc tttcacgccc cctctattta
       61 atacctcacc ttgtgtcact gccggatttg cgtgcacaaa atcgcccata ccattacagc
      121 agagaaagta agccatgctg aagtacagta aaacgccagg catctttaaa ttagaaggta
      181 atcggctcgg acgcccctac catcgcctgc cgaccctgtt tacagggaat tttgacgtga
      241 ttgattcgca tctgggcagc tactttctca aaaagcatcg cagcaatatc accctaaaaa
      301 agattggctg cgaaatggat attattaata aaaacgctga actgatggtt tcccaggtcg
      361 ggcatctggc gtttgatatt gatcgctctt tgctgttaat gctgctgggc aatttttatg
      421 gtctggagtc ctctcttgaa gaggcgaaag cgcataacgg gctgccgacc aaaaccgaaa
      481 cgcggttaaa aaaccgtctg gcgctggata tctgcacgct gatttttaat ctgcaaacct
      541 ccggtattgc gctgaaatta aagctggaca gcagcacggt aattacccac tgggcgtatc
      601 aattaacctt tacccttgcg ggcgatgaag agagctgttt tcgtattttg ctcgacgacg
      661 cccacaccga ttttatttta aatctgatcc gccacagcga gcacagtaaa ccgcagcagg
      721 tggcgaaatc ggtcaataaa ccggccctga ttaaagagat catccgctcc ctgccgctga
      781 ccctgaacgt caaaatcgcc gagctatcga tgaatgtcgc ggatctcacg cagataaaag
      841 ccggggacat tttacccatc tcgctgggag aaacgttccc ggtggcgatt gggcaatctg
      901 aattatttag cgccctgatt gtggaagaca aagacaaact gtttttgtcg gagctggcgg
      961 gcaaaaatga gaatagccat gagtaagcaa gaagatattt tagccgggga ttttggcctc
     1021 accgatgaag ttgcgcctgt cgcaaaatcc gccgacgctg aaacgctggt cacccgcctg
     1081 gaagaccgtt tttccgactc gatgacgctg ctaaaacgca ttccggtgac cttaacgctg
     1141 gaagtgtcat cagtagaaat aatgttagcc gacctcctta acatcgacga cgacacggtg
     1201 attgagctgg ataaactcgc cggggagccg ctggatatca aagtgaacaa tatcctgctg
     1261 ggcaaagcgg aagtggtggt ggtcaacgag aagtacggcc tgcgcgtgct ggaatttaac
     1321 acccgcgata tcaacgacct ggcaccatga aacgccgcac tcaactgacg ctgggccttg
     1381 ggctgctggc gcttgcgccg gtggcgttgg cccagggcgg cgatattgcg ctgcttaacg
     1441 tggtcaccca cggcaacact caggagtaca gcgtcaaaat ccaggtgctg attctgatga
     1501 ccctcgtcgg tttgctgcct acgatggtgc tgatgatgac ctgctttacg cgctttatta
     1561 tcgtgctgtc gctgctgcgc caggcgctag ggctacaaca aacgccgcca aaccgcattc
     1621 tgattggcat tgcgctctcc ctcaccatgc tggtgatgcg ccccatctgg ctcaatattt
     1681 acgaccacgc agtagtgccg tttgaaaatg atcagattac cctgaccgat gcgttaagca
     1741 ccgccgcgac gccactcaaa cgctttatgc tggcgcagac tgacaaaaaa gcgatggcgc
     1801 aaatcatgac tatcggcggc gcgaagggcc atgccgccga ccaggatctc tcgattgtgg
     1861 tgcccgccta tgtgttgagc gagctgaaaa ccgccttcca gattggcttt atgatttaca
     1921 tcccgtttct ggttatcgac ctgattgtcg ccagcgtact gatggcgatg gggatgatga
     1981 tgctgtcgcc tctgatcgtt tcgctaccct ttaagctgat gctgtttgtg ctgatcgacg
     2041 gctggtcgct gaccatcggt acactcacca ccagcattcg cgggctgggg ctgggctgat
     2101 gatgctgacc atagacgtag ccgccgacat tgtcgccagc ggcataaaag tggtgatttt
     2161 gctggtttcg gtgctggtag tgccgagcct gctggtcggc ctgctggtga gcgtgttcca
     2221 ggcggtaacg cagattaacg aacagaccct aagctttttg ccgaggctga ttgtcacgct
     2281 ggtggtgctg ggcgtgtgcg gtaaatggat gattatccag ttgcatgatc tctgcattca
     2341 cctgtttacc caggccgcgt tgctggttca ttgagatgcg cacaagcgac gttacgcaac
     2401 tgatggatct ggcgctgggg ctgtggtttc cctttgtgcg cattatggcg tttctgcgct
     2461 atgtgcctgt gctggataac agcgccctga ccgtgcgcgt gcggattatt ttgtcgctgg
     2521 cgctggcgct ggcaatcatc atcacgccgt tgatccccca tcctgttccc cacgacctga
     2581 tatcgctcaa cagcctgatc ctgacgatgg agcaaatttt atggggcatg ttattcgggc
     2641 tgatgtttca gtttttgttt ctggcgctac agctggccgg gcaaattctc tcgttcaata
     2701 tggggatgag catggcagtg atgaacgacc cgagcagtgg cgcatcgacc accgtgctgg
     2761 cggagctcat caacgtctac gcgatcctgc tgttttttgc catggacggg catctgctgc
     2821 tggtgagtgt gctgtataag ggctttacct actggccgat tggcaacgcg ctgcatccgc
     2881 aaaccctgcg caccatcgcg ctggccttta gctgggtgct ggcgtccgca tcactgctgg
     2941 cgctgccaac cacttttatc atgctgattg tgcagggctg ctttggcctg ctcaaccgca
     3001 ttgcgccgcc gctgaacctt ttttcactcg gttttcccat caacatgctg gcggggctgg
     3061 tctgctttgc caccctgctc tacaacctgc cggatcacta tctgcatctg gcgaattttg
     3121 tgttgcagca actcgacgcg ctgaaaggtc actatggcgg atagcagcag cgaagaaaaa
     3181 acagagaaac cctcggcgca aaagctgcgt aaagcgcgcg aagaggggca actgccgcgc
     3241 tcgaaggata tggggctggc ggcgagcctg tttgcggcat ttgtggtgat ctccagcagc
     3301 ttcccgtggt atgccgattt tgtgcgcgag agttttatca gcgtgcacca gtatgcacag
     3361 gagattaata acccagaggt gatcggccag tttctgcgcc atcacctgtt aattctcggc
     3421 aagtttattc tcaccttgct gccgatgccc gccgccgcgc tactctcctc actagtgccc
     3481 ggcggctggc tgtttctgcc taaaaaaatc ctcccggatt tcagcaaaat cagcccgtta
     3541 aaaggcattg ggcggctgtt ttccagtgaa catctggcgg aaacgggaaa gatgacggtg
     3601 aagtcggtgg tggtgctggt gatgctgtgg gtaagcctgc gcaataactt tgcctccttt
     3661 ctcggcctgc aggcgctacc gttcaaactg gcgatgaatg agggtttatc gctctacgcc
     3721 tgcgtgatgc gcaactttgt catcctgttt atcttctttg cgttaatcga cgtgccgctg
     3781 gcgaaagccc tgtttaccaa agggctgaag atgaccaaac aggagctgaa agaagagtat
     3841 aaaaaccagg aaggcaagcc ggaagtgaag gcgcgcgtgc ggcgtctgca acggcagttg
     3901 gcgatggggc aaattcgcaa agtggtgccg aaagccaacg tggtgatcac caacccgacc
     3961 cactacgccg tggcgttgca gtatgactcg tcgcgcgccg ccgcgccgtt tgtggtggca
     4021 aaaggcaccg atgaaatcgc cctctatatt cgccaggtcg ccgctgagaa tcaggtggaa
     4081 gtggtcgagt tcccgcgact ggcgcgctcg gtttactaca ccacccaggt gaaccagcaa
     4141 attccgtttc agctttaccg ggccatcgcc cacgttctga cctacgtcct gcaaatgaaa
     4201 cactggcgcg agggtgcaca gccgcgcccg gccctgaaca gacatatttc cattcccaaa
     4261 gaggtgctta aactggatgg cgaaaacaac taaatcgttc ctcgcgctgc tgcgcggcgg
     4321 caatcttggc gtgccgctgg tgatcctgtg tattctggcg atggttattt tgccgctgcc
     4381 gcctgcgctg ctggatattc tgttcacctt caacattgtg ctggcggtga tggtgctgct
     4441 ggtggcggtg tcggcgaaac gaccgctgga attcagcctg ttcccgacca ttttgctgat
     4501 caccacctta atgcgcctga cgctcaacgt cgcctctacg cgcgtggtgc tgctgcacgg
     4561 gcatctcggc gcggacgcgg cgggtaaggt gattgagtcg tttggtcagg tggtgatcgg
     4621 cggcaacttt gttgtcggct ttgtggtgtt tatcatcctg atgatcatca actttattgt
     4681 cgtcaccaaa ggggccgagc gtatttccga ggtttcagcc cgctttaccc tggatgcgat
     4741 gcccggtaag cagatggcga ttgacgccga tcttaatgcc gggttgatca atcaggcgca
     4801 ggcacaaacc cgacgtaaag atgttgccag cgaggccgat ttctacggcg cgatggacgg
     4861 ggcatcgaaa tttgtgcgcg gggatgctat cgccgggatg atgattctgg cgatcaacct
     4921 gatcggcggc gtctgtatcg ggatcttcaa atacaacctg agcgccgacg ccgccttcca
     4981 gcagtatgtg ctgatgacca tcggcgacgg gctggtggcg caaatccctt ccctgctact
     5041 ctccaccgcg gcggcgatta tcgtcacccg cgtcagcgac aacggcgata tcgcccatga
     5101 cgtgcgtcac cagttgctgg caagcccatc ggtgctctac accgctaccg gaattatgtt
     5161 cgtactggcg gtagtgcctg gaatgccaca tctgccgttt ttgctgttca gcgccctgct
     5221 tggctttacc ggctggcgga tgagcaaacg cccgcaggcg gcggaagcgg aagagaaaag
     5281 tctcgaaacg ctgacccgca ccatcaccga aaccagcgag caacaggtca gttgggaaac
     5341 cattccgctg atcgagccca tcagcttaag cctcggctac aagctggtgg cgctggtgga
     5401 caaagcccag ggcaacccgc tcacccagcg tattcgcggc gtgcgacagg tgatatccga
     5461 cggcaacggc gtgctgctgc cggagatccg cattcgggaa aacttccgcc ttaagcccag
     5521 ccagtacgct attttcatca acggcattaa ggctgatgaa gcggatattc cggcggataa
     5581 actgatggcg ctgccctcca gcgaaaccta cggcgagata gacggcgtgc tggggaacga
     5641 cccggcgtac gggatgccgg tgacctggat ccagcctgcg cagaaagcga aggcgctgaa
     5701 tatggggtat caggtgatcg acagcgccag cgtgattgct acccatgtaa ataagattgt
     5761 gcgcagctat attcctgatt tatttaacta tgacgatatt acgcagttgc ataaccgttt
     5821 ggcgtcgatg gcaccgcgcc tggcggagga tttaagcgcg gtgctcaatt acagccagtt
     5881 gctgaaagtg taccgggcgc tgctgaccga aggcgtttcc ctgcgcgata tcgtcaccat
     5941 cgccaccgtg ctggtcgcca gtagcgcggt gaccaaagat catattctgc tggcagccga
     6001 tgtgcgcctg gcgctgcggc gcagcattac ccatccgttc gttcgcaagc aggagctgac
     6061 ggtgtatacc ctgaataatg agctggaaaa tctgctgact aacctagtga atcaggcgca
     6121 acagggcggt aaagtgatgc tcgacagcgt gccagtggac ccgaatatgc tcaaccagtt
     6181 ccagagtacg atgccacagg tgaaagagca gatgaaagcg gcggggaaag acccggtgct
     6241 gctggtgcca ccgcagctgc gccctttgct ggcgcgttat gcaaggttgt ttgcgccggg
     6301 gctgcatgtg ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga ttatgggggc
     6361 gttgagttga tgggggattc ttttccatgc tttgcagcac ctgacaccat taattgacgt
     6421 cacactgcaa agagactacg atgacaccat acaatgacgt catggattga tagtatgatt
     6481 aaggtcagcg cgctaggttc tcgtcataga aaaacattcg acgatgtaat gacggagccg
     6541 ccaaaatctg gaattaaatg ggatgatgtt gttgcactca ttaaagctgt gggtggaaca
     6601 ataaaaaata acaacggctc cagacgaaaa tttcagattg gaacgaccaa attcagcaca
     6661 catgagccac atccaaaaaa tgtgatggat aaaggcgcag tagcaggtct taaggagtgg
     6721 tttgttaact gtgtaggggt agattatgaa taaagcatcg aacattatca caattaaagg
     6781 tcaaccagcc gcagtaacat ttgaagccga tatcaacgca tttcgcggca agttcctcaa
     6841 tgtaaacggt tattgtgact ttgtagccac aagtattgag gcattatatc gtgaaggcga
     6901 aagcgcgctg gatgagtgga tggcggattg tgaagaggac ggtattgccc cttttcgtga
     6961 agaagaagag caaaaacgcc tgacgcttcg tgttcctcat cacattgatt cgcggctgac
     7021 catcgtcgcc agacaacatt ccatttcaaa aaaccagctc attgttgatg tcttagaacg
     7081 tgagttttca gcccagatgt aaacaggtcg gatgccgcgt ggcatccgac cttttgccag
     7141 ttcgtcaata ttacccattt acaaaatcgt ctcctccgtc aaaatccgcc gcgcgcctaa
     7201 aaagtgctcc tgccagaaag gttcagctaa ctgacttacc cgaatggttt ccccggtgcg
     7261 cggtgactca ataaactgac cgtcgcccag atacaccccc atatggtcgg caatctcgcg
     7321 gctgtggata tggaaaaata gcaaatctcc ccggcgcagg tcgttattcg ccacaatggt
     7381 tgcccggcga tagtgataca tctcattagc cgtgcgtggg agtttcgcct caaggatctt
     7441 gttgtaggca taaaaaacta acccgctaca gtcgaagcct tgatcgggcc gcgtaccgcc
     7501 ccagacgtaa ggcttgccaa gctgttgctc cagacggtga atcgccactt cggtgatgtt
     7561 atgcagatgg tcgctgctta agaaatggtt gttttccgcc agcgcctgcc agcgtctgtc
     7621 actcgccttg agcggcgcag gaaaccattc tggatgctgt tttatctgtt cgcggttgtg
     7681 ctggcgtagc gcccttctgc tttcggcatt gccttccaca atatagctgg cctgtttttt
     7741 cagatgggtt tggtactgtt ttaataaacg cgcacgattt tgcatccgct ggcgggcggc
     7801 gtagctgaaa gtgatatgtg atgtggtggc gctggcaaat gaagaaaacg gcaagcaaag
     7861 caacagtaat cccgacaaca caaaagaggg aatcgacggc aaggacatac tcaataatgg
     7921 ctgatcatgc attgatgttg cagtgtatcc cggcccgtac ctgaacaaac ttaataaacc
     7981 attattataa aattaataaa tatatttcag ttaattatgt aaaaacatcc gtttattaaa
     8041 ttactcatgg attgtcgctg ttgctcattt gagctacaat tcaagctgaa taaatataca
     8101 gcacaggaga taccccaatg gctgctaacg catttgttcg cgcccgaatc gatgaagatc
     8161 tgaagaatca ggcggcggac gtactggccg ggatggggct gaccatctct gacctggttc
     8221 gcataaccct cacaaaggtc gcgcgtgaaa aggcattgcc gtttgattta cgcgagccta
     8281 atcaattaac cattcaatca atcaaaaaca gcgaagctgg cgttgatgtt cataaggcca
     8341 aagacgccga tgatttattt gataaattag gaatttaaat atgattcaaa gggatattga
     8401 atactcggga caattttcaa aggatgtaaa acttgcacaa aagcgtcata aggatatgaa
     8461 taaattgaaa tatcttatga cgcttcttat caataatgct ttaccgcttc cagctgttta
     8521 taaagaccac ccgctgcaag gttcatggaa aggttatcgc gatgctcatg tcgaaccgga
     8581 ctggatcctg atttacaaac ttaccgataa acttttacga tttgagagaa ctggaactca
     8641 cgcggcgctc tttgggtaaa gcacgctgcc gatgaaattt gtaaaccctg cgccgacagg
     8701 cttgcgtaaa actcttttgg tgataatgtg agccttccaa atccagacta ttccgatttg
     8761 ggatctattt tgccgtcctg gcgtgtgtaa ccgttttatc aaggaataag cagtatgcgt
     8821 aaaatcgcat taattcttgc gatgttgttg atcccgtgcg tttcgtttgc cggtttgctg
     8881 ggcagcagta gctcgaccac gccagtgagc aaagagtata agcagcagtt gatgggatcc
     8941 cctgtctaca tccagatctt caaggaagaa cgtacgctcg atctctacgt caaaatgggc
     9001 gagcaatatc agctgctcga cagttataaa atctgtaaat attccggcgg cttagggcca
     9061 aaacagcgtc agggcgattt caaaagcccg gaagggtttt atagcgtcca gcgtaatcag
     9121 ttaaaaccag acagccgtta ctacaaagcg attaatattg gtttccccaa tgcctatgac
     9181 cgtgcgcatg gttatgaagg gaaatacctg atgatccacg gcgattgtgt ttccatcggc
     9241 tgctacgcaa tgaccaatca gggtattgat gagatcttcc agttcgttac tggtgcgctg
     9301 gtgtttggtc agccgagcgt gcaagtgagt atttacccgt tccgcatgac cgacgccaat
     9361 atgaagcgcc ataaatattc caactttaag gacttctggg agcagctgaa gccgggctac
     9421 gactactttg agcaaacccg taagccaccg accgtttctg tggtcaatgg ccgttacgtg
     9481 gtcagcaagc cgttaagcca cgaagttgtg cagccacaac tggcatcaaa ctacacgctc
     9541 cccgaggcaa aataagcgcc attcgcctgg cataatcttt tgccaggttt cattgcccgt
     9601 cagcggctgt gttgcaatca ccgtgaccac atcattcggt gtggtctgcg agctgaagtc
     9661 gatttccaca tcctgatcca gcagcgttgc cacgccaaac ggcgcgcggc gggtgatcca
     9721 gtgtaaatta gtcgagcaat acgccatcac atagcgcccg tcagaaagca gcatgttgaa
     9781 aacgcccttc tgccgcagtt catccgccag tgaggcgata tatttaaaca ctgccgccat
     9841 gttgcccggt gtgcgcgggt aacgctgcgt taatttatgc aggagccagc agaaggcttt
     9901 ttcgctatcg gtttcaccga ccgggcggaa gttgccggtt tccagtgatt tgtagcccgt
     9961 cagttgtccg ttatgggcat aagtccagtt acgcccccat aactcgcggg taaatgggtg
    10021 agtattttcc agcgccacct cgccccgatt agcctggcga atatgagcca ccaccgaaca
    10081 ggattttatc gggtagtcct ggacaagttt ggcgatgggg gaattaaagc tgggttgtgg
    10141 atctttaaat gtgcgacagc ctttaccttc gtaaaaggta atgccccagc catctttatg
    10201 tggcccggtt ccaccaccac gctgtacaag tccggtgaaa ctaaagcaga tatcggtagg
    10261 gacgttggcg ctcatcccga gcaattcgca catgggtaac ctccacaaca gcgggagcga
    10321 atggcccccg gcgaagattg atttaagaat tttcaggtcg gatgcggcgt aaacgaatta
    10381 ttcggcctac ggttcgggta cagatttgta ggcatgataa gacgcgtcag catcgcatca
    10441 ggcatcagcg cacaagtgcc ggatgcggcg taaacgcctt atccagccta cgccagactt
    10501 acttaaccat ctctttttca attaactgga tcaggatatg gatcacttta atgtgaatct
    10561 cctgaatgcg gtcggcataa ccaaagtgcg gtacgcgaat ttcgatatcc gccgtgccag
    10621 ccattttgcc gccgtcttta ccggtcaggg tgatcacttt catccccttc tcacgcgccg
    10681 ctgcgatcgc tttgatcacg tttgcagagt taccggaggt ggagatcccc agcagtacat
    10741 cgccttcgcg acctaccgct tcaacgtagc gggagaaaat atcattgaaa ccgaaatcat
    10801 taccgacgca ggaaatatga ctaacgtcag aaatagcaat cgccgggtag cccggacggt
    10861 tttcacggta gcgaccggtc aactcttcgg caaagtgcat agcgtcgcaa tgggaaccgc
    10921 cgttgccgca ggaaagcact ttgccaccgg ctttaaagct gtctgctaac aggaccgccg
    10981 cgcgctgaat ggcgtgaata ttggcgtcat cttttaaaaa gttagccagc gtttccgccg
    11041 cttcgttcag ttcgttacga ataagatcct ggtacatgag gatatccttc agcataaatg
    11101 taatagacaa aatgcagtgt accggatacc gccaaaagcg agaagtacgg gcaggtgcta
    11161 tgaccaggac tttttgacct gaagtgcgga taaaaacagc aacaatgtga gctttgttgt
    11221 aattatattg taaatacatt gctaaatgtt tttacatcca ctacaaccat atcatcacaa
    11281 gtggtcagac ctcctacaag taaggggctt ttcgttatga tgattttgag tattctcgct
    11341 acggttgtcc tgctcggcgc gttgttctat caccgcgtga gcttatttat cagcagtctg
    11401 attttgctcg cctggacagc ctccctcggc gttgctggtc tgtggtcggc gtgggtactg
    11461 gtgcctctgg ccattatcct cgtgccattt aactttgcgc ctatgcgtaa gtcgatgatt
    11521 tccgcgccgg tatttcgcgg tttccgtaag gtgatgccgc cgatgtcgcg cactgagaaa
    11581 gaagcgattg atgcgggcac cacctggtgg gagggcgact tgttccaggg caagccggac
    11641 tggaaaaagc tgcataacta tccgcagccg cgcctgaccg ccgaagagca agcgtttctc
    11701 gacggcccgg tagaagaagc ctgccggatg gcgaatgatt tccagatcac ccatgagctg
    11761 gcggatctgc cgccggagtt gtgggcgtac cttaaagagc atcgtttctt cgcgatgatc
    11821 atcaaaaaag agtacggcgg gctggagttc tcggcttatg cccagtctcg cgtgctgcaa
    11881 aaactctccg gcgtgagcgg gatcctggcg attaccgtcg gcgtgccaaa ctcattaggc
    11941 ccgggcgaac tgttgcaaca ttacggcact gacgagcaga aagatcacta tctgccgcgt
    12001 ctggcgcgtg gtcaggagat cccctgcttt gcactgacca gcccggaagc gggttccgat
    12061 gcgggcgcga ttccggacac cgggattgtc tgcatgggcg aatggcaggg ccagcaggtg
    12121 ctggggatgc gtctgacctg gaacaaacgc tacattacgc tggcaccgat tgcgactgtg
    12181 cttgggctgg cgtttaaact ctccgacccg gaaaagctgc tgggtggcgc agaagattta
    12241 ggtattacct gtgcgctgat cccgaccacc acgccgggcg tggaaattgg tcgtcgccac
    12301 ttcccgctga acgtaccgtt ccagaacgga ccgacgcgcg gtaaagatgt cttcgtaccg
    12361 atcgattaca tcatcggtgg gccgaaaatg gccgggcaag gctggcggat gctggtggag
    12421 tgcctctcgg taggccgcgg catcaccctg ccttccaact caaccggcgg cgtgaaatcg
    12481 gtagcgctgg caacgggcgc gtatgctcac attcgccgtc agttcaaaat ctctatcggt
    12541 aagatggaag ggattgaaga gccgctggcg cgtattgccg gtaatgccta cgtgatggat
    12601 gctgcggcat cgctgattac ctacggcatt atgctcggcg aaaaacctgc cgtgctgtcg
    12661 gctatcgtta agtatcactg tacccaccgc gggcagcagt cgattattga tgcgatggat
    12721 atcactggtg gcaaaggcat tatgctcggg cagagcaact tcctggcgcg tgcttaccag
    12781 ggcgcaccga ttgccatcac cgttgaaggg gccaacattc tgacccgcag catgatgatc
    12841 ttcggacaag gagcgattcg ttgccatccg tacgtgctgg aagagatgga agcagcgaag
    12901 aacaatgacg tcaacgcgtt cgataaactg ttgttcaaac atatcggtca cgtcggtagc
    12961 aacaaagttc gcagcttctg gctgggcctg acgcgcggtt taaccagcag cacgccaacc
    13021 ggcgatgcca ctaaacgcta ctatcagcac ctgaaccgcc tgagcgccaa cctcgccctg
    13081 ctttctgatg tctcgatggc tgtgctgggc ggcagcctga aacgtcgtga gcgcatctcg
    13141 gcacgtctgg gggatatttt aagccagctt tacctcgcct ctgccgttct gaagcgttat
    13201 gacgacgaag gccgtaatga agccgacctg ccgctggtgc actggggcgt acaagatgcg
    13261 ctgtatcagg ctgaacaggc gatggatgat ttactgcaaa acttcccgaa ccgcgtggtt
    13321 gccgggctgc tgaatgtggt gatcttcccg accggacgtc attatctggc accttctgac
    13381 aagctggatc ataaagtggc gaagatttta caagtgccga acgccacccg ttcccgcatt
    13441 ggtcgcggtc agtacctgac gccgagcgag cataatccgg ttggcttgct ggaagaggcg
    13501 ctggtggatg tgattgccgc cgacccaatt catcagcgga tctgtaaaga gctgggtaaa
    13561 aacctgccgt ttacccgtct ggatgaactg gcgcacaacg cgctggcgaa ggggctgatt
    13621 gataaagatg aagccgctat tctggtgaaa gctgaagaaa gccgtctgcg cagtattaac
    13681 gttgatgact ttgatccgga agagctggcg acgaagccgg taaagttgcc ggagaaagtg
    13741 cggaaagttg aagccgcgta attgaataac ggagccgaaa ggctccgttt ctttatccgc
    13801 taattattta aaattaaagc catccggatg gttttccagg ctgccggtca acgccgcgaa
    13861 cagcaccgtt ttaccatcaa tcgaaagcgc atcgttcaca ttcagccagg tgagtttctc
    13921 ttgcgacgtt ttctcatcaa tagtcgagaa cagccccgtc atctgattag atttctcgga
    13981 ccacatcaca gcgatacgtt gcgagccaca gtcatgcggt ttgcacgcgc tcatcacctg
    14041 atacgtctca tctcccaacg ttacggtttg tgcgggagta taagtaccgc ctttcatcac
    14101 ccaggcaggc agcttatgcc cttgtaccat ctgattaaat gcagctttgg tggtttcgcc
    14161 ctttgcaagg ctgctaatgg ttaaatcatc ctgcgccatt gcactggtgg cgatgaccag
    14221 agcggcgact gtcgttattg ccttaaacat cattcctccc gagcttatcc tgcccatgtt
    14281 attaaccttc taaagcctgg cgataacacg ataatttgca aatcagatat ttccaatatc
    14341 agcatttgtt atcaatttat tctgcgctaa ggcaactgcc tgataaggtg ttaaagttat
    14401 aaaacccttc gcataaagga gttgtacacc gtgcctggtt tgaagattac gcttttgcag
    14461 caaccactgg tgtggatgga tggccctgcc aacctgcgtc attttgatcg tcaactggaa
    14521 ggtattaccg ggcgcgatgt gatcgttcta ccggagatgt ttaccagcgg ctttgccatg
    14581 gaagcggcag cttcgtcgct agcacaagat gacgtagtga actggatgac agctaaggcg
    14641 cagcagtgca atgcgctgat tgcaggcagt gttgcattac aaacggagtc tggttcggtt
    14701 aaccgctttt tgctggttga gccgggcggc acggtacatt tttatgataa gcgtcatctg
    14761 ttccgcatgg cagatgagca tctacattat aaagcgggca atgcgcgagt gattgtggaa
    14821 tggcgcggct ggcgtatttt gccgctggtg tgctacgact tacgttttcc tgtgtggtcg
    14881 cgcaatctca acgattatga cctcgccctg tacgtcgcca actggcctgc tccgcgctct
    14941 ctgcactggc aggcattgct gacggcccgc gcgattgaga atcaggcgta tgtggcggga
    15001 tgcaatcgcg tcggcagcga tggcaacggc tgccattatc gcggtgacag ccgggtgatt
    15061 aatccacaag gcgagattat cgctactgcc gacgcgcatc aggcaacgcg cattgatgcc
    15121 gagctgtcga tggcggtgtt aagggaatat cgcgataagt ttccggcatg gcgggatgcg
    15181 gatgagttta ggttgtagtg agtgggggct gtatttaggg cggggaattc agaaagggat
    15241 gtttagtgtg ctgagcgaga gttaaataat aaaaaactgg gaagatttaa taatcccccc
    15301 gcagggcaag agcatgaatt aaatttgtgt taaatttttc attttatcag ttcgaaaccc
    15361 aagctctgag caggatgcaa gcacttcttc tatataacat gtgtttaacg ccagggcaag
    15421 attacgaaag cccgctcccc gcaaggactg acgccagata gtttctgtcc atggctgctt
    15481 ttcgcatctt acgtcttaac cctgccttga ataccttatc attcgtcaaa atattaatag
    15541 caatgtgccg tatccctgaa aataattctg ctgcatttcc tcttcttatt ttgcagtcgt
    15601 cttcattcat taccacgtcc agacgccagt gcagcttatt ctccacgtgc cagtgatttc
    15661 ggatcgctgt ggcgaatttc tcagcggtta aatcagcaga actgatataa tatctgaccg
    15721 tcatttctgg ctctttcttt tgttctgcta ttatggaccg aaaggagact gccacgcata
    15781 atttcttcag ccctttccat tcaaacgtga aatcaataag ttcatcaggg acatcgcaaa
    15841 caatatgaag acggatttct tctctgccgt gactcttttc actaattgcg taactgtcat
    15901 gctctggatt atttaattct ttcagcggaa atttttcctc aaaggcttta tttagccgcc
    15961 cctggtttcc ttttaccgcg aataaataat cacctccctg tttttgtatc ttctctgcaa
    16021 tatctttctg gcaacccatc gcatcagttg tgatgatttt tcctttaata tccagcatgt
    16081 taagaagttc tgggatagct gtaatctcat tagatttctc atccgtcctg atctgtccga
    16141 tgaccagact gtgcattgtt gagaacgcac taatgacatg aatcgctccc ctgcggcgac
    16201 tcttgtcata agagtgccgg agcgtttttc catcaattgc gatgacgtct ttatcatctg
    16261 aagaatggca gtcacgcatc cagttaataa agcactcatg aaattttgca ggactgatac
    16321 aggatacaac tctggcaata gtatcgtgaa caggaatatc attttcaaaa tcaccatatt
    16381 gcttcaagaa atcgagatgt gtttccccga aatcctctat atcttcccaa ctttctgcac
    16441 cagaaataac ggcaaaaata gtcaatagta ggatgcctga caatttatgc tctactttcc
    16501 aggcttatct gtaatcggga ataatagaaa tatgttccat caatttttta agttccattt
    16561 gttcacctta tttatgtaag gagtatttga tcatgtataa gcaataaaaa acagcttcag
    16621 gtaatgagga atatctcaat ttttaaacat aaaatgcgaa ttatttagta caaaaagtga
    16681 acttctgatg atctttccct gtgtttaacg cagccttctt tcttttcctc ttcaccctta
    16741 atgtctttgc aatcccttaa taaattcagt gccatcttct ttattccaga tattatttag
    16801 gctgcgtttc ctcttcttat ccggctggca tcttcattca tttttacatc taacacccaa
    16861 tgaagactgt gctcgatcag ccagtgcgct ctgatagcat gtgcaaattc tttagcatcc
    16921 atatcctttg atgaaatata atatcggatg cttacacctt ctgctgattt atcttctttc
    16981 ttctgcctga atgacaatgc tacacaaagc ttttttaatc ccttccattc gaattcaaaa
    17041 tcacaaaagt tcaggcgtta cgttactgac aatatgcaaa cgtgtttctt ttcttccatg
    17101 acttatctcc tgcgtactaa acgaatcgcc tttataatta gaaaacacat ttacaggaaa
    17161 tttttcctcg aatgcatgat gtaatttccc ctgattgcct tttactgcca gaagataatc
    17221 tgctttttta tctttgatct tcgaagcgat atctttctga cagcccatag catcaatggt
    17281 tatcaaattt ttctttaaat acagtaggtt aagcaactct ggaatggctg taatctcatt
    17341 acttttggct tccgttttca cctgccccag tacaacacca ttttcgttcg agaatgcact
    17401 caccatatgg attgctcctt ttctttttcc cttatcaaag gagcctctta tggtctttcc
    17461 atctattgct ataatttcac catcagtgat ttcatggcac tcctgcatcc attcaataaa
    17521 catcttttca aaggccaaac tgtcaatgtt actcacaacg cgtgcaatgg tgtcatcgac
    17581 cggaatgcca ttatcaaaat caccatattt ctttagccat tcaagtcttt catgtccaaa
    17641 atcttcaatt tcctgccact catcggcacc tgcaattact gcacatacgg tgagaaacaa
    17701 aatagcagat aatttatgtt taacctttcc ttgttgtcgt atatcaggga tcactgaaat
    17761 ataatcaagc aaactttgaa tactcacctg gcgtcctttt ttatcttttt agttattcca
    17821 aaggttaaat tttgatcacc tgtaggcgtt aaagccaaga tgaaaatcgt gatttacatc
    17881 acaattttca tgatcttgcc ctgccccgtc ccttgctctc atgtctttct gtatgtattt
    17941 tatatttttc tctttct
//
LOCUS       DAZGTR010000092        17424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGTR010000092 DAZGTR010000000
VERSION     DAZGTR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.092"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(153..1154)
                     /locus_tag="ACHLCY_003430"
     CDS             complement(153..1154)
                     /locus_tag="ACHLCY_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0370614.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1261..1557
                     /locus_tag="ACHLCY_003431"
     CDS             1261..1557
                     /locus_tag="ACHLCY_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0370615.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1691..2116)
                     /gene="arsC"
                     /locus_tag="ACHLCY_003432"
     CDS             complement(1691..2116)
                     /gene="arsC"
                     /locus_tag="ACHLCY_003432"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP0370616.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAPKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2129..3418)
                     /gene="arsB"
                     /locus_tag="ACHLCY_003433"
     CDS             complement(2129..3418)
                     /gene="arsB"
                     /locus_tag="ACHLCY_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP0370617.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3472..3825)
                     /gene="arsR"
                     /locus_tag="ACHLCY_003434"
     CDS             complement(3472..3825)
                     /gene="arsR"
                     /locus_tag="ACHLCY_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP0370618.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4334..4417
                     /gene="dinQ"
                     /locus_tag="ACHLCY_003435"
     CDS             4334..4417
                     /gene="dinQ"
                     /locus_tag="ACHLCY_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP0370619.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4471..5823)
                     /gene="gorA"
                     /locus_tag="ACHLCY_003436"
     CDS             complement(4471..5823)
                     /gene="gorA"
                     /locus_tag="ACHLCY_003436"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP0370620.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5895..6737)
                     /gene="rlmJ"
                     /locus_tag="ACHLCY_003437"
     CDS             complement(5895..6737)
                     /gene="rlmJ"
                     /locus_tag="ACHLCY_003437"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP0370621.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6940..8982
                     /gene="prlC"
                     /locus_tag="ACHLCY_003438"
     CDS             6940..8982
                     /gene="prlC"
                     /locus_tag="ACHLCY_003438"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP0370622.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYNAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8990..9742
                     /gene="rsmJ"
                     /locus_tag="ACHLCY_003439"
     CDS             8990..9742
                     /gene="rsmJ"
                     /locus_tag="ACHLCY_003439"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP0370623.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9791..11260)
                     /gene="dtpB"
                     /locus_tag="ACHLCY_003440"
     CDS             complement(9791..11260)
                     /gene="dtpB"
                     /locus_tag="ACHLCY_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP0370624.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11426..11479
                     /locus_tag="ACHLCY_003441"
     CDS             11426..11479
                     /locus_tag="ACHLCY_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370625.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11578..12012)
                     /gene="uspA"
                     /locus_tag="ACHLCY_003442"
     CDS             complement(11578..12012)
                     /gene="uspA"
                     /locus_tag="ACHLCY_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP0370626.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12403..12738
                     /gene="uspB"
                     /locus_tag="ACHLCY_003443"
     CDS             12403..12738
                     /gene="uspB"
                     /locus_tag="ACHLCY_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP0370627.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12983..14482)
                     /gene="pitA"
                     /locus_tag="ACHLCY_003444"
     CDS             complement(12983..14482)
                     /gene="pitA"
                     /locus_tag="ACHLCY_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP0370628.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPSVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14714..15916
                     /gene="yhiN"
                     /locus_tag="ACHLCY_003445"
     CDS             14714..15916
                     /gene="yhiN"
                     /locus_tag="ACHLCY_003445"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0370629.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16232..17284)
                     /gene="yhiM"
                     /locus_tag="ACHLCY_003446"
     CDS             complement(16232..17284)
                     /gene="yhiM"
                     /locus_tag="ACHLCY_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP0370630.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVASHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4534 a         4406 c         4468 g         4016 t
ORIGIN      
        1 ttgtctgttg atgacttgtt gaccgagccg tcatcattct caatgcatta gttaaatcat
       61 ttgtgatttt taaggattat cctgtctatg attttctact tagaaggata tggcactaac
      121 tatgcatctt atagtttact gctggcatct atttaaaata gcaattgagt caaatagccc
      181 atcaagatag ccatacctaa tataaccgtc aggaatgcag ctatcatcgg cattctgaac
      241 attgatttaa gtaatatcac ttccgtcagg cttgcgccgg cactgccgat gattaatgcc
      301 attaatgctc ccagacccat tccttttgtc atcagaacag atgccagagg gataactgcc
      361 tcagcccgga tatacagcgg aataccgaca acggcgctta atgggatggc gaagggatta
      421 tctgctcctg catgagcggc aatccacgct gagggaataa agccataaat aaaagatcct
      481 attaatactc ctaacaaaag atatggcagt acatctttaa actgttgcaa cgcatctttg
      541 gtggctagtt ttatgggatt aatacttact acagctttgg tactgcaaca ggtaactacg
      601 gggttctcaa tggctttagc cgctggacta caacaactca cttttatggg cgtatatgtc
      661 gttcccggcg aagtttttgc tggagcacaa caatttgctg atgagctttt actggcaatg
      721 atatgacgtt caaaccccag agaatccagg ataatactgg caagaacgga gacgccggct
      781 gcgataatcg cgtacaacaa ggtgactttc caaccaaagg tcatccacat tagtccgacg
      841 ataatgggat taagtaatgg tgaaacaaat aaaaaagtga gagtcggacc aaacccggct
      901 ttcgctgaca acaatccacg taacatgggt attgtcgagc aactacagaa cggggtaacg
      961 gctcccaaca gagcagccag gagatagcct ctcccttttc tggcccccat catctgctgg
     1021 attttatggt ctggcacctt ttgtcttatc aggctgacac cggcactaat cactataaac
     1081 agcaacgaaa gctcgacggc gagaaacaca aacatctctg cggcatcttg cagcatcgca
     1141 agccaactac tcatacgtaa cctcattgga taaatctata tttccagaat aatcgaaatg
     1201 aaattcagat caagatattt ctggtattat cgaaatatca accagacaga ggggtgtcat
     1261 atgcagttgg aagaagtcgc taaagcgctg aaagagctag gtcacccgac ccgtttgttc
     1321 atatttaaac atcttgtcaa agccggggaa caagggttgc cggtcggtga gttgcaaaag
     1381 cagcttggga tacctggctc cacactcagc caccatatat cagcactggt atcggtagga
     1441 ctggtcaaac aaaaccgtga gagtcggacg ctgatgtgtg tttcgcaata cgcaatgctg
     1501 gaagcgataa ttgaattctt acgcgaggag tgctgcgtaa atagcaaaat tgcctgatgc
     1561 gctacgctct acatatttca tgccatatat tgaatttgca cggttttgta ggtcggataa
     1621 gacgcgttag cgtcgcatcc gacataaaca aagcgcactt taccaactat cggcagtgga
     1681 tatcgccgct ttatttcagg cgcttacccg cttcatcaac cactttctcg ccatcttcct
     1741 tggagaatgc gccttttggc gcatctggca gaatttccag caccacttct gaagggcggc
     1801 acaggcgagt tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc gggtgctgaa
     1861 gcataaagtc gattaaccga tcgtcagtaa atttatcttc cgcaaggccc agctcctcat
     1921 acggttcgac gtttttacgc agcagcgcgc gtacggaaat ccccatatcg gcaatgagtt
     1981 tgaccagttc atcgcgcgtt ggcggagttt ccagataatg gataatggtc ggttctgtgc
     2041 cgctgttgcg gatcatctcc agcgtattac gcgacgtgcc gcaggccggg ttgtgataaa
     2101 tggtaatgtt gctcatatca gtatctcatt acaaagtgaa agagagacgt agcgccagcg
     2161 cagccagcgt cacaaacagc acaggcaggg tcatgataat ccctgtacgg aagtaatatc
     2221 cccagctgat agtcatattc ttctgcgaaa gtacgtgcag ccagagtagc gtagccaggc
     2281 taccaattgg ggtaattttc ggtcccaaat cgcagccaat cacattggca taaaccatcg
     2341 cttctttgat aacgccagat gccgtgctgc catcaatgga caacgcgcca accagtaccg
     2401 tcggcatatt gttcataata gaagagagga aggcggtgag gaatccggtg ccgagcgtcg
     2461 cggcccacag gccgttatcc gccagcacgt tgagtacgcc agaaagatat tccgttaatc
     2521 cggcattgcg caggccataa accaccagat acatgccgag cgagaagatg acaatctgcc
     2581 agggggcacc gcgcaggact ttacccgtat taatcgcatg accgcgttta gcgacgacaa
     2641 ataatatcag cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc ggttccagga
     2701 cgaaaaatcc caccagcaga agcagtaaaa caacccagcc agttttgaac gtagcaggat
     2761 ctttgatcgc ttctgcggga gatttcagca gcgccatatc gtagttctgc ggaatatctt
     2821 tgcgaaaata gagatgtaac atcaccagcg tggcaacaat cgcggcgata tccaccggca
     2881 ccatcaccga ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa acgatattca
     2941 ccaagttgga gacaataagc ggcaggctgg cggtatcggc aatgaatccg gccgccatca
     3001 cgaacgccag cgtagtgcct ttactgaacc ctaaagccag cagcatggcg atgacaatcg
     3061 gtgtcaaaat aagcgccgcg ccatcattgg caaacagggc ggcaacggca gcaccgagca
     3121 ggacaatcca ggtaaacagc aagcgaccac gaccattacc ccagcgtgag acgtgcagcg
     3181 ccgcccattc aaaaaagccg gactcatcca gcagcaggct gatgataatg acggcgataa
     3241 acgcagccgt cgcgttccag acgatattcc acaccaccgg aatatcaccc ggatggacca
     3301 cgcccgtaac taacgccagt actgcgccga gcgttgcact ccagccgatg cctaaacctt
     3361 tcggctgcca gataaccaat acgatggtca ggacaaagat agcgcctgcc agtaacataa
     3421 tgcctcccgg ataaaacaca tctgaaaatt catatgtgtt tagctaaatt tttaactgca
     3481 aatgttctta ctgtccccgg aacagttttg tcgagccagg ttgcggacaa tcgcctgaac
     3541 cttttcctgt tcacatcgcc aggcctcatc aataattttc gccgcccatg ctggaatatg
     3601 cggtgataag cggtaatgaa cccacttacc ttgcttgcgg tccagcaata gcccgctttc
     3661 acgcagcaat gccaggtggc gggagatctt gggctgcgac tggtcgagag cagtgcagag
     3721 atcgcagacg cataactctc ccagttcgct gagcagtaaa acgatgccca gacgggtttc
     3781 atcagcaaga attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt
     3841 gttgcaggta gtgtctctct tcgaagcgga taagtcaaaa acatatatga cttaacgaat
     3901 gtgtaagtgc agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga
     3961 ggtctgaatc aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga
     4021 gttggtggca ctggctggag tgagaatgcc attatctaac gcatcaatgc taataccata
     4081 ctgaaactat tgcaaggaca tgctggtttt ataacctgaa tgtactgtat gattatccag
     4141 ttagctctga ggcattttca ctctggtaat gcgcataaac gctttcaaag tcctggtcag
     4201 aagtacgggt ggtgccgtta actgatgctc tggccggagt gagaatgcca ttatctaaca
     4261 atagggcatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc actccatcgc
     4321 atggagaaaa cgggtgattg ataaagcaat catcgttctt ggggcgttaa ttgcgctgct
     4381 ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta attaagggct
     4441 aagagcacac tactcttagc cctttaacat ttaacgcatt gtcacgaact cttctgccgc
     4501 cgttgggtga atggcgacgg tattgtcgaa gtcttttttg gttgcaccca tcttcagcgc
     4561 caccgcgaag ccctgcaaca tttcgtccat accaaagcca atgccgtgaa taccgacaat
     4621 cttctcttcc gatccaacgc acaccagctt catgcggcac ggctggcggt gagtggtgac
     4681 ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt cgccatactg
     4741 ctcgcgcgcc tgcggttccg ttaaaccaac agtaccaatc ggcggatggc tgaagaccac
     4801 ggtcggaatg ttgctgtaat ccagatgctc atccggcttg ttattaaaca ggcgttcaga
     4861 gagacggcga cccgctgcaa ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc
     4921 caccgcgtaa ataccttcaa tattggtgtt ttgatattta tcgacgacga tatagccttt
     4981 ttcgttagtt ttaacgccag cggcttccag gttgatgttg tcattggcag gctcgcgacc
     5041 aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct ccagcgtcag
     5101 gctaccatcg gcatttttca ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc
     5161 ttcggcgttc atcacttcga ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg
     5221 cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag
     5281 ctcaacggcg atgtaacccg cgccaacaac cgccacgcgc tctggcaaag caggaagggc
     5341 gaagaagcca tcagaatcaa taccgtattc cacgcccgga atatccgggt ggctcggacg
     5401 accgcctgtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttta cctccagcgt
     5461 tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt taccgagcac
     5521 gttttcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga tcaacgtttc
     5581 ccagttgaat ttattgatag tggtatcaaa accataatcc gggccgtaca tatggatcgc
     5641 ttcacggatt tgcgccgcgt gccacatcac ttttttcggc acacagccaa catttacgca
     5701 ggtgccgccc agctctttgg cttcaatcag cgcacatttc tggccgtaca tagccgcgcg
     5761 gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat agtgtttagt
     5821 catgacaaag tgtccttatc gttgattacc gcgattgtag cgcgagacgt aataggtgcc
     5881 agcaatggct gcaattactc cggcacgatc cagcttacgg tggcgtgccc ggtgcctgcc
     5941 ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgctgttc cagtttccac
     6001 ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct gtctggcagt
     6061 accgccagtt caatttgcag aattttgcga ataccggtcg cttccagatc gtggatcatg
     6121 cgcttaattt gctgacgcag caccaccgga taccacagtg cgtaagtacc agtggcgaaa
     6181 cgtttgtaac cttctgctat cccgctgacc accgcctgat agtcggtttt catttcatac
     6241 ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagtttggc cttaagctgc
     6301 tggaaaccat cggctttttc gacgcgcgca cggctgtctt tctgaaattc agaacgcagc
     6361 aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcttgttc gcgcagtagc
     6421 tggcgagcaa tcaatggcga accggggtag taacgcaact gaccgctacg gttgaagtgt
     6481 tttaccacat tgatgtacgc ctccagttct gcgggcaaat cgtcctgctg ccagatacgg
     6541 gcgatgcctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc
     6601 ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt cagcgactcg
     6661 atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg
     6721 tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg
     6781 gcgcacaaaa gcgtatcagg acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac
     6841 gtttctcatt gaaattcact acacttaacc ccatgctaca cacattatgt aaagcgcctg
     6901 ttgagcgctt ccttaacctc tttaaccagg actgcgcgaa tgacgaatcc gttactgact
     6961 ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc agccgtgact
     7021 aaggcgctga acgactgccg cgaaaatgtg gagcgcgtag tagcgcaagg agcaccgtac
     7081 acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgctggg gcgtatcttc
     7141 tccccggtca gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga agcgtacgaa
     7201 caaaccctgc cgctgctgtc ggaatacagc acctgggtag ggcaacatga agggctgtat
     7261 aacgcgtatc gcgacctgcg cgatggcgat cattacgcca cgctgaacac ggctcagaaa
     7321 aaagcggttg ataacgcact gcgcgacttc gaactctctg gcatcggtct gccaatagag
     7381 aaacaacaac gctacggtga gattgccact cgtctttccg agctgggcaa ccagtacagc
     7441 aacaacgtcc tcgatgcgac gatgggctgg accaaactcg ttaccgacga agcggagctg
     7501 gcggggatgc ctgaaagcgc gctggctgcg gcaaaagccc aggccgaagc gaaagagctg
     7561 gaaggctacc tgctgacgct ggatatccca agctacctgc cggtaatgac ctactgcgac
     7621 aaccaggcct tgcgtgaaga gatgtatcgc gcttacagca cccgcgcctc cgatcaaggc
     7681 ccgaacgccg gtaaatggga taacagcaag gtgatggaag agatcctcgc tctgcgtcac
     7741 gaactggcgc aactgctggg ctttgaaaac tacgccttta aatcccttgc cactaaaatg
     7801 gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg cgcgcgtccg
     7861 caaggcgaaa aagagctggc acaactgcgc gccttcgcca aagcggaatt tggcgtcgat
     7921 gagttgcagc cgtgggatat cgcgtactac agcgaaaaac aaaaacagca cctctacagc
     7981 atcagcgacg agcagctgcg tccgtacttc ccagaaaaca aagcggttaa cggcctgttt
     8041 gaagtggtga aacgtattta cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg
     8101 catccggatg tacgtttctt cgaactgtat gacgaaaata acgaactgcg cggcagcttc
     8161 tacctcgatc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta
     8221 ggccagatgc gtaaagctga tggttcgctg caaaaaccgg tcgcgtattt gacttgtaac
     8281 ttcaaccgcc cggtaaatgg taaaccggcg ctgttcactc acgacgaagt gatcaccctg
     8341 ttccacgagt tcggtcacgg cctgcaccat atgctgaccc gcatcgaaac cgctggtgtt
     8401 tccggtatca gcggtgtgcc gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac
     8461 tggtgctggg agccggaggc gctggcgttt atctccggtc actatgaaac cggcgaaccg
     8521 ctgccgaaag agttgctgga taaaatgctg gcggcgaaga attaccaggc ggcgctgttt
     8581 attctgcgtc agctggagtt cggcctgttt gatttccgcc ttcatgccga gttccgcccg
     8641 gatcaggggg caaaaatcct cgaaactctg gcagaaatca agaaactggt tgccgtggtg
     8701 ccatctccgt cctggggccg tttcccgcac gctttcagcc atattttcgc cggtggttat
     8761 gcggcaggtt actacagcta cctgtgggct gacgtactgg cggcagatgc tttctcgcgc
     8821 tttgaggaag agggcatttt caaccgtgaa accgggcagt cgttcctcga caacattctg
     8881 agccgtggcg gttcagaaga gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg
     8941 cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag tgaaaatctg
     9001 cttaattgat gaaacaggcg ccggagacgg cgccttatct gttctggcgg cccgctgggg
     9061 gctggagcac gatgaagaca acctgatggc gctggtgtta acgccggaac atctggaatt
     9121 gcgcaagcgt gatgagccaa aacttggcgg catatttgtt gattttgttg gcggagcgat
     9181 ggcgcaccga cgcaaattcg gcggtggtcg cggtgaggcg gtggcgaaag cggtgggcat
     9241 taaaggcgat tatttgccgg atgtggtgga cgccaccgca gggctggggc gcgatgcctt
     9301 tgtgctggct tcagtcggtt gccgcgtgcg gatgctggag cgtaatccag tcgttgccgc
     9361 attgctcgac gacggcctgg cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca
     9421 ggagcgtttg cagttaattc acgcctccag cctgacggcg ctaaccgata ttaccccacg
     9481 cccgcaggtg gtctatctcg acccgatgtt cccgcataag cagaaaagcg cgctggtgaa
     9541 aaaagagatg cgtgtctttc agtcgctggt ggggccggat cttgatgccg atggattgct
     9601 ggagcctgca cgcttgctgg cgaccaaacg cgtggtagta aagcgcccgg actacgcgcc
     9661 accgctagcg aatgtcgcca cgccaaacgc ggttgtgact aaagggcatc gctttgatat
     9721 ctatgcaggt acgcccgtgt aaaagtaaag cagggtagcg ataacgctac cctgctttct
     9781 gcaagaataa ttaatggctt tccggcgtcg caatcatgcg tttcagccac ggcaccatca
     9841 acagcatcac tactgcaacg cccagcgtga ccagaccaat cttaccaaac acgttggtat
     9901 agacgggcaa cgtctcaagc ggatcggtaa tgttgtccgg caccgcggta aatgttgcca
     9961 catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg agaataaagc
    10021 ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag gccaagggcg ctaataaaca
    10081 gttcacctaa gctctggaat aagtacacca gcacgataaa ccatggcgat gtcagccctt
    10141 gtgcatccgc aaaccacatt cccgcagctg ccgccgtcaa aaagcccagt gagcacataa
    10201 acatgccgag agtaaatttc atcggcatcg agaggtcttt gcctttgtta cccagatgcg
    10261 tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta agcgcctgga
    10321 agctgaccgg gttgatggaa aaaccgagaa tttcatgatg cacgttgttg atggcaaaga
    10381 agttcagcga tgttggcatc tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca
    10441 ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat gcctgacgaa
    10501 agaagatgat ggtgacgacg atggagagaa caatcagcac cagattggcg acttctacgt
    10561 tgtgcatcag ccatgcacat acgaagatca tcaccacgct gccaagtaat acgtacaaca
    10621 gtttgctgaa gctcatcggc ttgaagtcgg gttcagaacc aatgtctttc accattccac
    10681 gacaggcgat gtaaaccagt aatgcgataa ttaaccccgc cccgcacagg ttgtaggtga
    10741 ctgaataacc gaatctatca gcgatcacag gggccagcga taacgctatc aacgagccga
    10801 tattgatcga catatagaac agggtgaatg cgccatcaag ccgcggatct ttcggcggat
    10861 agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttaccg acagcgatag
    10921 tccccagggc gatgaaaatc aggtcaggct taagtagcga catgccggtc atgaagtagc
    10981 caatcgccag cacaagtgct ccgagaacaa tggtgcgttt ggtccccagc aggtggtcgc
    11041 cgacatagcc gccaatggaa atgaggccat agaccagcgc agcaaaagca ccaaaagtga
    11101 caaaagcctg ctcttgcgag aatccaagct gtttaacgaa gaaaaccgcc agtacgccct
    11161 gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg aaaaatgggc
    11221 gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac cttccaatac
    11281 catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa aaagtttaca tctgtcagag
    11341 attcttaata ttgatgcggt aaatagatta atacactatt acaacagaaa ataaccagct
    11401 gatcacacta atgatggaaa atatgatggt gttaaatgtt gtattggcag gagattttga
    11461 ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta acaaggcgcg
    11521 ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga gggatgatta
    11581 ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt tgatcagctg
    11641 acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc
    11701 catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc tgccgctcag
    11761 ggtttcagtg attgggtagc ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc
    11821 ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg tgtataggtc
    11881 agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc gagccataga
    11941 gactgctttc tctaccagaa ctttgctttc cggggagaga tcgactgcga tgagaatgtg
    12001 tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca gctagcgttt
    12061 cttgtgccgt tacgtcaccc gcgtcctgcg aactatgtgc gcggggctaa cccagccgta
    12121 aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg tcaatccgcc
    12181 ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg gttaaccttc
    12241 tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat gttggtcacc
    12301 acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg ggagcggtag
    12361 ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag caccgtcgca
    12421 ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt ctcatcacta
    12481 cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata tgttgatgga
    12541 gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc
    12601 tatgcccaac gttatcgcga tcatcacgat gatgaattta ttcgtcgctg tgagcgggtg
    12661 cgtcggcagt ttattctgac cagcgcattg tgtggtctgg tggtggtcag cctgattgca
    12721 ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc gggccagtaa
    12781 actgacccgc tcgagactga ttacaaacgc aaaattgcct gatgcgctac gcttatcagt
    12841 atctacataa tctctgcaat ctattgaatt tgcatgtttt tgtaggccgg ataaggcgtt
    12901 cacgccgcat ccggcatgaa caaagcgcac tatgtcacaa tctgaagcgg gccagctgac
    12961 ccgctcgttt tggtgtgtac gattacagga actgcaagga gagccagtac agtccgccgg
    13021 aaagcagtac cgcagccgga agggtaaaca cccaggccat cagaatactg gttacggttt
    13081 tacgctgtaa gccgccgcca tctaccacca tcgtccccgc gacagaagag gagagtacgt
    13141 gagtagtgga aaccggcatc ccggtataac tcgccaggcc gatagacact gccgccgtca
    13201 tctgggccga catcccctga gcgtaggtca tgcctttctt accgattttc tcaccgatag
    13261 tcgttgccac acggcgccag ccaatcatcg taccgatacc taacgccagc gcgaccgcca
    13321 tgatgatcca caccggtgca tactcgatgg tgctaagcat gtcggacttc agtttcttca
    13381 acaggcgctg atcgtcagca ctcacactag gcatcttcac cactttgtcg atagtgtcag
    13441 aaacgcacag cataatgcgg cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc
    13501 tttccacatc ggtagtcaac atgcctttca ggcggttgag cgcgttaatg gtattcgacg
    13561 gatggcaatg gaactccgcg ggttgcgttg cgccagcttc cggagccggt actaactgat
    13621 cagcaccggt agcctgtttg agcagcgcag gatgctgctc aaagtaagct tcgacgttgt
    13681 tgatggcatc acgggtacgg gtgatttcgt agccagtggc attcatgttc accacgaagc
    13741 ctgctggtgc gacgccaatc aataccaaca taaccagacc aatgcctttc tgaccatcgt
    13801 tcgcgccgtg cgaaaacgcc acgccgatag cggaaaggat cagcgcaata cgcgtccaga
    13861 acggcggctt tttcttgccg tctttctttt cacgctccgc tggggtcagg tggatacggg
    13921 cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca
    13981 ccaggccgac aataggggaa acgatcagag aaccgaaaat acttaatact ttcgggatat
    14041 tgagtgcatc caccactgac gtcccggtca tcaacgcatt ggttaaacca atcccgatga
    14101 tcgcgccaat cagcgtatga gagctggatg caggtaaacc aaagtaccag gtacccaggt
    14161 tccagataat cgccgccagc aacatagaga acaccatggc aaggccatga gacgatccca
    14221 tattaagcag cagatccgtc ggcagcatat gcacaatggc ataggcaaca ctcagaccac
    14281 ccagcaaaac acccaaaaag ttgaacaccg ctgccataac cacggcgagc tgagaacgca
    14341 tcgcgcgggt atagataacg gttgccacgg cgttggctgt gtcatggaaa ccattgatgg
    14401 cttcgtagaa cagcacaaaa gccagtgcaa gcaataataa cagcccggta tgcaaatcca
    14461 ggccagcaaa caaatgtagc ataggacgtt acgccatttt gaggacatga acgcggcgca
    14521 ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag actttttttg atttgcctcc
    14581 tgtatggatt tcactcaaaa aataattatc ttatataatt caggcaaata cttcctttta
    14641 gtaatattga tgctggtgcg accactgagg aatctttaca attcacgccc gttttttcta
    14701 agaggagcgc aacgtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat
    14761 gttctgttct gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa
    14821 aaaaccaggg cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta
    14881 tgtcgaacca ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg
    14941 ttttacccag tgggatttca ttgatctggt caataaacac ggcatcgcct ggcacgagaa
    15001 aacgttagga caactcttct gcgatgactc cgcgcagcag attgtcgaca tgctggtgga
    15061 tgagtgcgag aagggcaatg taaccttcag attgcgtagc gaagtgctga gtgtggcgaa
    15121 ggatgaaaca ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg aaaagctggt
    15181 catcgcgacc ggtgggctgt caatgccggg gctgggcgcg tcgccgtttg gttataagat
    15241 tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc cattcactct
    15301 gcataaaccg ttgctcgaag agttacaggt gctggcgggc gtggcggtgc cttccgtgat
    15361 taccgctgaa aacggcatcg ttttccgtga gaacttactc ttcacccatc gcggcttgtc
    15421 tggaccggcg gtgttgcaga tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa
    15481 tctgctaccg gatgtggatc tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa
    15541 tcagagcctg aaaaacacac tggcggttca tctaccgaag cggttggttg aacgcttaca
    15601 gcaactcggg caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg accaacaggc
    15661 actgattagc acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac
    15721 tgccgaagtg acgctcggcg gcgtggacac caacgaactc tcttcacgga cgatggaagc
    15781 gcgcaaagtg cctgggctgt acttcatcgg cgaagtgatg gacgttaccg gctggctggg
    15841 gggctataac ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc
    15901 agcgaagtcg tcctgacctg ttacctaaag atgaaaatta ttagagccgc attaaaaatg
    15961 agctaatttt gatagtggct atcttgtgat tattttctaa tgagcccgtg aactgaaacc
    16021 ctccaggctt aatataaggt ggaaggaaag gtgattgaaa actcacccag tggccagatc
    16081 tttataaaat atgaacaggt tgaaaaaaac agtaactttg ttgttttttt atccttaacc
    16141 actatgcatt aatgctgcgt tatttcatga tgcctaagaa aaaccagcgt tacgcaaatg
    16201 gtcaacgctg gttttatccg gtacgttgca attatttttt agcagaacct gcttctaata
    16261 ttgaaacgat tgagaacaac gtaaagcata ccgctcctaa accgaccaaa acccgcgaag
    16321 gaataaaata tcccatgtcg gtctgcgcca tttccgcaag aaacgatgcc aggaaaaggc
    16381 aaaacaggca ggtgaagacg ggaatcatcg gtattctgtt ggctaacgaa caggtacgtc
    16441 tccataccag tgccagtagc cagacttttg agaatatgct gtaacagatc attccgaggc
    16501 aaataagaat aatgccggga gccagtcggg cgcttgcatc ggaactgact aagacgtata
    16561 tcccctgcag taccgtgatt gagccgagaa aaataaccca ataacaccac agccagtgtt
    16621 ctttagttga aaacgtatta cgtgtttgat ggacaattgt ggcaacaagg ccaattagac
    16681 aggcacagat tgcggttaac ccaagcaata cgtgactcgc gacataatgc ggagttatgt
    16741 cagcactacg taacagcgta atagaccaaa tgaaaccgag cagggtgagt aaaacgggaa
    16801 cggcaatgag gcaattaccg attaatgaag aataagcctg taccggcgtt ccatcgctct
    16861 tgctccccgc tgcatttttg ggaatgagca gaaagtgacc ggatgacgct gccaccgtcg
    16921 atacacaggc ggcaatcata ccaacgccga aaataacatg gccggcgaca aactcgtctg
    16981 ccatcacatc attgcctgct aacagtgccc aaccccatat catggtgata attgaccccg
    17041 catagccaat aatggggaac aatgtattgt aaaacgtatt aacgccgcgc gtgagctgcg
    17101 aaatgataat aaatgcggta gtgaataatg ccaggcatat ggctgccaga gaaatcagca
    17161 catgacccgc aacgaaatac tcactttgcc cagaactttc cagcacaaag ccacctaacg
    17221 cgatacaaat taagcccata attaaaggga ttaatttgaa aagccacccg atatatatgt
    17281 tcactttaaa ctcctcaggt atccctgcta atattgaaat gtgcattaat gataatttca
    17341 gcctgttaat ataatgacga gatatcatta aagacaaatg catagcataa ctatagcggg
    17401 atttactttg cagagcgcta cttt
//
LOCUS       DAZGTR010000093        16657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGTR010000093 DAZGTR010000000
VERSION     DAZGTR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.093"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(79..231)
                     /locus_tag="ACHLCY_003447"
     CDS             complement(79..231)
                     /locus_tag="ACHLCY_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0370631.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(496..1230)
                     /gene="cysH"
                     /locus_tag="ACHLCY_003448"
     CDS             complement(496..1230)
                     /gene="cysH"
                     /locus_tag="ACHLCY_003448"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0370632.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHLCY_003449"
     CDS             complement(1304..3016)
                     /gene="cysI"
                     /locus_tag="ACHLCY_003449"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP0370633.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHLCY_003450"
     CDS             complement(3016..4815)
                     /gene="cysJ"
                     /locus_tag="ACHLCY_003450"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP0370634.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEKAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5134..5496
                     /gene="queD"
                     /locus_tag="ACHLCY_003451"
     CDS             5134..5496
                     /gene="queD"
                     /locus_tag="ACHLCY_003451"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP0370635.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5574..6845
                     /gene="ygcN"
                     /locus_tag="ACHLCY_003452"
     CDS             5574..6845
                     /gene="ygcN"
                     /locus_tag="ACHLCY_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0370636.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6836..7096
                     /gene="ygcO"
                     /locus_tag="ACHLCY_003453"
     CDS             6836..7096
                     /gene="ygcO"
                     /locus_tag="ACHLCY_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0370637.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7113..7688
                     /gene="ygcP"
                     /locus_tag="ACHLCY_003454"
     CDS             7113..7688
                     /gene="ygcP"
                     /locus_tag="ACHLCY_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP0370638.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7836..8696)
                     /gene="ygcQ"
                     /locus_tag="ACHLCY_003455"
     CDS             complement(7836..8696)
                     /gene="ygcQ"
                     /locus_tag="ACHLCY_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0370639.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAVKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8693..9472)
                     /gene="ygcR"
                     /locus_tag="ACHLCY_003456"
     CDS             complement(8693..9472)
                     /gene="ygcR"
                     /locus_tag="ACHLCY_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP0370640.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRIEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9450..10859)
                     /gene="ygcS"
                     /locus_tag="ACHLCY_003457"
     CDS             complement(9450..10859)
                     /gene="ygcS"
                     /locus_tag="ACHLCY_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0370641.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10881..12335)
                     /gene="ygcU"
                     /locus_tag="ACHLCY_003458"
     CDS             complement(10881..12335)
                     /gene="ygcU"
                     /locus_tag="ACHLCY_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP0370642.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12405..13190)
                     /gene="ygcW"
                     /locus_tag="ACHLCY_003459"
     CDS             complement(12405..13190)
                     /gene="ygcW"
                     /locus_tag="ACHLCY_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0370643.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDSMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13509..14786
                     /gene="yqcE"
                     /locus_tag="ACHLCY_003460"
     CDS             13509..14786
                     /gene="yqcE"
                     /locus_tag="ACHLCY_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP0370644.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEVRTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14812..16290
                     /gene="ygcE"
                     /locus_tag="ACHLCY_003461"
     CDS             14812..16290
                     /gene="ygcE"
                     /locus_tag="ACHLCY_003461"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP0370645.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3979 a         4366 c         4187 g         4125 t
ORIGIN      
        1 aaatataaat tttaaaaaag ggtgcaggag accaaaccat cacaattcag tacgcggggt
       61 tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat
      121 accacgttct ctgatactca actcacacaa cgagtcacct accatcagcg tgaatccaag
      181 tactgtacaa cacagtacga tgattgccac aagggcatat tttgtcagca tgtcttgcct
      241 ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc ctcaggttga
      301 tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca
      361 gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt tcacgtttaa
      421 aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa
      481 tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc caaagaaacg
      541 tgtttcttct tccgacatgc caggttccca tttacgggtt gtatgggtat cgcccaccga
      601 taaatatcct tcgtcccata atgggtgata tttcaggcca tgtttttgca gatactgata
      661 tatcgttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc
      721 cagcaccggt aaattggcgc ggctgccgga ttgttcgcgg cgcaggccag caaaccacgt
      781 ttgcgcattc agttctttca gcgcccggtt catcggttcg actttgttga tgtcattgta
      841 cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt cctgccaggc
      901 ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg ttaactcgtc
      961 aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca ccggaatatc
     1021 cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa agctggaaga
     1081 aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt cagcgtccag
     1141 tttttccagt tcggcgttag tttctgccag cgccagaatg cgatcgacct tcggcagttc
     1201 gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc
     1261 gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc
     1321 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
     1381 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
     1441 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc
     1501 caagatgcag gttgtagcga cccggcgctt tacccaccag gcccacttcc gccagcatcg
     1561 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
     1621 catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctctgctt
     1681 ccgccatcgc cagcgggcaa gtcgggaacg agacgcaggc catcgagttt tcacgctgcg
     1741 gcgtgacggc attcattaac ccgctctctt tggcgatctt ctcgatcttc gctttctcgc
     1801 tttccggtac accggcgatg atcagattct ggttcgccgt aatgcggaaa tcgcctttgt
     1861 ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa tcaaggatgc
     1921 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
     1981 tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg atccccgcgc
     2041 gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg tatttggttt
     2101 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg acggcttcgg
     2161 ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg cgggcgtagg
     2221 ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg aagcctacca
     2281 gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga tcgatatcgt
     2341 tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc tgaccaagga
     2401 tcggttcttc atcagtagtg gcgacttttt cgtggtcgag ccagatctcc gcatacgcgc
     2461 gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct tccgcgtgca
     2521 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttagctgtcg
     2581 ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca
     2641 gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
     2701 tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc ccacccggca
     2761 gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc
     2821 ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttgtcgc
     2881 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
     2941 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
     3001 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
     3061 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
     3121 tgccttctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc
     3181 atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt gtacgtaaac
     3241 tttttctttt tggtcgcgcg accaggcaag atcgatacgt gtcagcacgc catctttgac
     3301 gtagcgctgc cactccacct gatacaggaa gtcttccgta aagtgcggat taccaaagaa
     3361 cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc tgcataaagg cgcggaacgg
     3421 cgcaataccg gtgcctgggc caatcataat caccggggtt tccgggttag cgggcaggcg
     3481 aaagttatcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca cgcgatccgc
     3541 gaggaagctg gaggcaccac cggcacgggc tcggccttcc acgtcgtaac gcaccacacc
     3601 aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcgatgg aatacaggcg
     3661 cggggtcagc gggcgcagca gattaatcag cgcttcggca tccagttgcg ccggagagaa
     3721 acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
     3781 caccagcggc agcagtgttt cgctgcgggt aagcgtggcg taattctcaa caatgttggc
     3841 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
     3901 ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt ctttcaccag
     3961 tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac gcaggcccga
     4021 gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc cggtaatttt
     4081 ctggttaaca gagaggctag ccaccagcgg cgcgtctttg ctgtacgggc tggtgtggat
     4141 ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
     4201 aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt caacatcggc
     4261 atcgacacgg tcgagcaggc gttcaccacc cagttccgcc agcttgctgt cgaaatcttt
     4321 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaacgcggt
     4381 gttttccagc tttggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
     4441 cggcggttcc ccttcccctt gcgttgacgt cactacgatg agcagttttt cgctggcgat
     4501 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ctgctaataa
     4561 atcatcgcgt aatgcttcag caacccggcg cgcattgccg gtttgcgagg cggagataat
     4621 agttataccc ggcatttctg cggctggcgc tggcgtcgct gcaagcgcag caggctgctg
     4681 attgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag ttaaatcggt
     4741 cgtggccgcc tgaaggcgtg ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg
     4801 gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
     4861 gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg attaaagacg
     4921 ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca
     4981 aaatagatta accaacctaa tgaaaaacaa atgaatttag ccaatcatta agataaatca
     5041 gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct
     5101 acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt taaagatttc
     5161 accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg tggtcgcctg
     5221 cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc
     5281 tggattatcg atttcgctga actaaaagcg gcgtttaaac caacctacga gcgcctcgat
     5341 caccattatc tcaatgatat tccaggtctg gaaaacccaa ccagcgaggt tttagcaaaa
     5401 tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa
     5461 acctgtaccg caggttgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt
     5521 cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat gacatggaag
     5581 acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc gcgttacgct
     5641 gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa
     5701 atctttccgg cgggcggtta tatacccatg cactcgcgga actcctcccg caatttcatc
     5761 tgaccgcgcc tcttgaacga cgcatcactc acgaaagcct ttccctgtta acgccggatg
     5821 gcgcaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac
     5881 ggttcgatcc gtggctggtt gccgaagccg aagaagaagg tgtcgaatgc atccccggag
     5941 cgacggtgga tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg
     6001 atattctccg tgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctggctgagc
     6061 gtcacgggtt agtgactcgt cctgctggtg aagcgatggc gttggggatc aaagaagtgc
     6121 tgtcgctgga accatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag
     6181 cgttgctgtt cagcggcagg atctgtgatg acttacccgg cggcgcattt ctttatacta
     6241 atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg caaagtcgtg
     6301 ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc ccgcttatca
     6361 aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc ttgcacagta
     6421 tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg
     6481 tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgcg caggcggcgg
     6541 cacaaacgct gataagcgcc tgccagcacc gcgagccgca aaatctgttt gcgctttatc
     6601 atcacaacgt agagcgcagc ctgctatggg atgttctaca acgttatcag catgttccgg
     6661 cgcttttgca acgccctggc tggtatcggg cgtggcctgc gttaatgcag gatatttccc
     6721 gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta ttctggcgtc
     6781 atttacgtcg tcatggcctg tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc
     6841 tgtagcccat aatctctggc gcgttgctga tgcgccgcac attgttccgg ctgactccgt
     6901 tgagcgtcag atggtacaac ggttgattaa cgcctgtccg gcaggtcttt tttcgctcac
     6961 accggaaggt gacttacgcg ttgattatca cggctgcctg gagtgtggca cctgccgttt
     7021 gctgtgcgac gaatccacac tacaacagtg gcgctatccg ccttccggat tcggcatcac
     7081 ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg ctccgccaga
     7141 atccggtgat tgctgccgtt aaagataatg ccagcctgca actggcaatc gactctgaat
     7201 gccagtttat ttccgtgttg tacggcaata tctgcaccat tagtaatatc gttaaaaaga
     7261 ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata
     7321 aagaagtcgt gattcagttt ttgaaactgg tgaccgaggc ggacggcatt atcagtacca
     7381 aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat cgcctgttta
     7441 ttgttgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt
     7501 gtattgagat cctgccaggc tgtatgccca aagtgctggg ctgggtgaca gagaaaatcc
     7561 gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta
     7621 acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta gcgaaaaaat
     7681 tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc cgcacgccac
     7741 atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta caagtcccca
     7801 ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc gtgaatgttt
     7861 gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc tgcctgtgaa
     7921 aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc
     7981 gccattaacg ctgctgcgcc agaagcgcca acaacaatac acacctcagg tgccagcaga
     8041 tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac tcgcgcccga
     8101 ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg gttatctgcc
     8161 tcaccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc acgggtaaca
     8221 tttttcagat cctctgtgct aaccagccag tcgggaagcg cgccggggac aatgttcagt
     8281 tgctgcatac cagagggcaa cgtggcgttt ttcactgctc ccgcctgacg cgccagggaa
     8341 aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc ccagtgggat
     8401 ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct tgcaccatgt
     8461 aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg aaagagaaca
     8521 acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg ttccgccact
     8581 acaggctgag gttcaatctg ccagtgcgcc agggtacaac cgctgaaatc ctgcgccgcc
     8641 agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat attcatggct
     8701 gcatcctttg gcgtaaatag tcctgccata gcttttgagc tttttctgct accgtttgcc
     8761 catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga cactgcatag
     8821 ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca gccattcgct
     8881 ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca atcaccgcag
     8941 gcaagcgaac ccggcaacaa cgcaaaccat gttcaatacg ctgttcgagg gtgataaaca
     9001 gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc atttccgcca
     9061 gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta attatcagat
     9121 ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc
     9181 gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc atgagatagc
     9241 gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac
     9301 cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca ccgagtaaac
     9361 ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc cactcttttt
     9421 ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct
     9481 cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca tagccaggtc
     9541 acaacaaaac caaccaacaa cactgtcgcc aggagcaata aggtgacttg cattccccac
     9601 tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg actcatggcg
     9661 gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg
     9721 cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt
     9781 aatgaactgc cggaaggcaa acaggccatt actaccagcg ttgccgccag cagcaaaaaa
     9841 cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc cagcagcgcg
     9901 cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata
     9961 gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca
    10021 aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa ggttttgatg
    10081 tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc aaaatagcga
    10141 tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggtgattct
    10201 ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagtagc
    10261 cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact tgccagcaca
    10321 tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg gcggcgcggg
    10381 gaaaattcag ccagcaaggt gtgacctact gaataatcgc ctcccagacc aatgccaatc
    10441 aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgtaa aaacgaagca
    10501 agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg gtcggagatc
    10561 cacccaagaa ccaggctgcc aaggaacaaa ccaaggagcg ccgagccgcc gatcattccc
    10621 gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta accaatgacg
    10681 ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat gcggcagtga
    10741 aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca
    10801 ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccgtcatc
    10861 gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt attcataatg
    10921 ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct gccgtgttcc
    10981 agtttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat cgaaccgccg
    11041 aggcgaatgg tttcttcaca gatgatcttg ttgagcggat tgtggtactt gtcgatttcc
    11101 tcttccggct tacagtcaac gacgttgtaa tcgtagacga agtacatgtt ggtgccgttc
    11161 tgatagctat gagaggaatg accgcccagc atggtgatgt cgtcggcgtg cgggaactca
    11221 gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca gcagccggac
    11281 acttcagtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagccact
    11341 ttatccggtc cccagttcag gttgttaaac caggtttcga tcagtttgct gtccacgcgc
    11401 tggcactgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc cttcgcaatg
    11461 cgagggttac cttcagccat aaagatcagc acacattttc cgtctgcaaa atgggtgaag
    11521 tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata cccttccacc
    11581 atgacttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata gccgtagaag
    11641 aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat atagcaaagt
    11701 gcaccttcgt taccgatgat gatgtggcga atgtccgggc cagccgcgcg gcgtggcacg
    11761 tttttaatgc gtgtgacggt gccatcagcc aatactgctt ccagaccaac gaccatatct
    11821 tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac caggccgccc
    11881 atctgcgcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac
    11941 gcattttcca gcacttccag gggaacgcca cattgcgccg tcgcctgcat attctcaata
    12001 tcaatattaa tgatttgatt catggcggaa ccgtcgagca ccacagagtt ttctacaaca
    12061 gtttccagcc caccttcagt ggcggaagcg ccggtacgcg gcacaccgtt aattttgtgc
    12121 gcattcataa aattcagcac acgggagact tgttctgtgg aaccgagttt tacgaccgct
    12181 gccggaatcg gcaaagtata aataccatga atatccggaa atttacgaaa acggtcaata
    12241 ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac aatttccttt
    12301 agttggtcga caatcgctgc gcgagataaa gacatagtaa ttccttcctg ataataaaaa
    12361 tggaataatc attaataaga gaaaacagaa accaccgtta tggattagcg cactaaataa
    12421 ccaccatcga ccactaataa atgcccattg acataatttg aagccggact tgcgaggaat
    12481 acagctgcgc ccattaaatc ctgagtatcg ccccaacggt ttgccggaat atgatcaaga
    12541 acgcgctgat tggtttctgg attactgcgt gtcgccagcg taatatctgt tgcataatag
    12601 ccaggggcaa taccatttac ctgaatatta tattgaccta gttcatcaca ataagctttg
    12661 gtgaatccgg caagagcatg tttagtggca gaatatgcag gtgaccattg tccacctaag
    12721 taagagaaca atgaacagat attaataatt ttgccgcttt tctgcgggat cataattttt
    12781 gcagcttcat agcttaactc gaatgcggcg gtcaggttca catcaatcat cgaatcccag
    12841 tcggcacgac cgaagtccag caccttattc agcttacaaa taccggcatt gtttaccaga
    12901 atatcaactg taccgaaacg ctcacagcaa gcagcgataa tcttctgcgg cgcgccttct
    12961 gcggtgatat ccacctgcat gaagtccacc tcaacaccct gtttttcaat catttccttt
    13021 gtttcgccgt tatctttgac gaaactagga ataaagatat ttgcgccagc tttggccaac
    13081 gccatggcaa atgcctggcc taaaccgcta ttcccaccgg taacaattgc ggttttacct
    13141 ttcagtgaga aaaaatccat tgagaacgca ttgagagatt cgattgacat agttagctcc
    13201 aatttccgta aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga agttaccttt
    13261 ctcattatct ctggtaactg gagataattt ttagcataac ggcataactg ctaatgtgat
    13321 cgtaatcaca gtgtgatatt cgtattgtca aatagtttta aaaagcagaa gcgtgattac
    13381 tctcacacaa cacctctgct ttccatgtta tttcttattg caattactag agacctgaga
    13441 aaagtaatcc acctgaaagg gcggcttact tttctctttt ttttgtctat tacaaatggg
    13501 aatacgatat gcaacacaac tcatatcgcc gttggataac cctcgctata attagtttta
    13561 gcggcggcgt tagttttgac ctggcttatt tacgttatat ttatcaaatt cccatggcga
    13621 aatttatggg attcagcaat accgagatag gtttaataat gagtaccttt ggtattgcgg
    13681 ctattattct ttatgccccc agcggcgtta ttgccgataa attttcacac cgcaaaatga
    13741 ttacttccgc gatgatcatt accggattac tgggtctgtt aatggcaacg tatccaccgc
    13801 tgtgggtaat gctctgtatt caggtcgcct ttgcgataac gacgatttta atgctgtggt
    13861 cggtgtcgat taaagccgcc tcgttgctgg gcgatcatag tgagcaaggg aaaattatgg
    13921 gctggatgga agggctgcgc ggcgtcggtg taatgtcgct ggcggtgttt accatgtggg
    13981 tcttttctcg ctttgcaccg gatgacagcg ccagcctgaa aacagtcatt atcatctaca
    14041 gtgtggttta catcttgttg gggattctgt gctggttttt tgttagcgat aacaacaacc
    14101 tgcgcagtgc caataacgaa gaaaaacagt cattccagct tagcgacatc cttgccgttt
    14161 tgcgtatcag caccacctgg tattgcagca tggtgatttt tggtgtcttc acaatctacg
    14221 ccattctgag ttactccacc aactatctga ccgaaatgta cggcatgtcg ctggtggcag
    14281 cgagctacat ggggattgtg atcaacaaaa tcttccgcgc gctgtgcggc ccacttggcg
    14341 gcattatcac cacctacagt aaagtgaaat cccctacccg cgtgatccaa atcctttccg
    14401 tactcggcct gctggcgtta actgccctgc tcgtcacgaa ctctaacccg caatcggtcg
    14461 cgatggggat tggcctgatt ttactgctgg gattcacctg ttacgcctca cgcgggctgt
    14521 actgggcctg ccctggcgaa gtgagaacac cgtcttacat tatgggcacc acggtaggta
    14581 tttgttcggt gattggattc ctgccggatg tcttcgttta cccgattatc ggccactggc
    14641 aagacactct gcccgcagca gaagcctacc gcaatatgtg gctgatggga atggcggcgc
    14701 ttgccatggt gattgtcttt acctttttgc tgttccaaaa aattcgtact gctgatagcg
    14761 cccccgcaat ggctagcagc aagtaagcca ggaaaattac aggagaaaat catgtcgaaa
    14821 aaatacatca tagggattga tggcggaagt cagagcacaa aagtggtgat gtacgatctg
    14881 gaaggtaatg tggtttgcga aggtaaaggc ttattacagc cgatgcacac gccttatgcc
    14941 gatactgcag aacatcctga cgacgattta tgggcatcat tatgttttgc aggtcacgat
    15001 ttgatgagcc agtttgccgg gaataaagaa gatattgttg gcatcggcct gggctccatc
    15061 cgttgctgcc gtgcgttatt gaaagccgat ggcacgcctg ctgcgccgtt gattagctgg
    15121 caggatgcac gcgttacacg cccttacgaa catacgaatc ctgacgtggc gtatgtcacc
    15181 tctttttcgg gttatctgac gcatcgctta accggcgagt ttaaagacaa tatcgccaac
    15241 tattttggtc agtggccggt ggattataag agctgggcat ggagcgaaga tgctgcggta
    15301 atggataagt ttaatatccc ccgtcatatg ctgtttgatg tgcaaatgcc tggcaccgtc
    15361 ctcggacata tcacaccaca agccgcactg gcgacacatt tcccggcagg actgccggtt
    15421 gtttgtacca ccagtgataa accggtagaa gctctggggg ccggattact ggatgatgaa
    15481 actgcggtaa tttctcttgg cacttacatc gcattgatga tgaacggcaa agcactgccg
    15541 aaagatccgg tggcgtactg gccgattatg tcttctattc cgcaaacatt gctgtatgaa
    15601 ggttacggta ttcgcaaagg tatgtggacg gtgagctggc tgcgtgacat gttaggcgag
    15661 tcgttaattc aggatgccag ggcgcaggat ctttcaccgg aagatttact caacaagaaa
    15721 gcctctagtg tgccacctgg ctgtaatggg ctgatgacgg tgctggactg gctgaccaat
    15781 ccgtgggaac cgtacaaacg cgggattatg atcggctttg attccagcat ggattacgca
    15841 tggatatatc gttcgatatt ggaaagcgtg gcgctaacgc tgaagaacaa ttacgacaat
    15901 atgtgtaatg aaatgaatca ctttgcgaag catgtgatca ttactggcgg cggttcgaac
    15961 agcgatctgt ttatgcaaat ttttgccgac gtgttcaacc ttccggcacg ccgtaacgcc
    16021 attaacggtt gtgcaagtct gggagcagcg attaatacag cggtaggtct ggggctatac
    16081 ccggattacg caacggctgt tgataagatg gttcgcgtga aagatatctt tataccgatt
    16141 gagagcaatg ccaaacgcta cgacgcgatg aataaaggca ttttcaaaga cctaaccaaa
    16201 catactgatg tgatcctgaa aaaatcgtat gaagtgatgc atggggaatt ggggaatgtg
    16261 gattcgatcc agagctggtc gaatgcgtga atgaggtagg ccgggcggaa aactgcccgg
    16321 cctgaacata cctaaatggt tatccccgct gacgcgggga acataagttc gacaacttaa
    16381 aaaatgataa caagtccggt ttatccccgc tgatgcaggg aacataataa aaactttaac
    16441 tagttcggtt tatccctgtt tttacaagga atattaacta cactcgatgt ctttagaatt
    16501 aaagacaata cgaatcgcac tttatcaatt ttcacttaaa gtatgaactg gatatcttta
    16561 ttcataagaa ctattcatca acagcgtata gaggcggtta tgtaaaacca ctcattagcc
    16621 tcaaaacaaa gacaaacttc cagatttatt acttaac
//
LOCUS       DAZGTR010000094        16342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGTR010000094 DAZGTR010000000
VERSION     DAZGTR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.094"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1197)
                     /locus_tag="ACHLCY_003462"
     CDS             complement(<1..1197)
                     /locus_tag="ACHLCY_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0370646.1"
                     /translation="MALTDAKIRAAKPSDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRARKLISEGIDPCEQKRAKKVVPDLQLSFEHIARR
                     WHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIAS
                     RLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYK
                     GQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKM
                     RTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQ
                     EVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQW
                     WADFLDA"
     gene            complement(1395..1470)
                     /locus_tag="ACHLCY_003463"
     tRNA            complement(1395..1470)
                     /locus_tag="ACHLCY_003463"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1435..1437),aa:Phe,seq:gaa)
     gene            complement(1576..2283)
                     /gene="yqgA"
                     /locus_tag="ACHLCY_003464"
     CDS             complement(1576..2283)
                     /gene="yqgA"
                     /locus_tag="ACHLCY_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP0370647.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWDAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2436..2519
                     /gene="yqgH"
                     /locus_tag="ACHLCY_003465"
     CDS             2436..2519
                     /gene="yqgH"
                     /locus_tag="ACHLCY_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFP0370648.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2681..4816
                     /gene="speC"
                     /locus_tag="ACHLCY_003466"
     CDS             2681..4816
                     /gene="speC"
                     /locus_tag="ACHLCY_003466"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP0370649.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLGLESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4866..6122)
                     /gene="nupG"
                     /locus_tag="ACHLCY_003467"
     CDS             complement(4866..6122)
                     /gene="nupG"
                     /locus_tag="ACHLCY_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP0370650.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6324..7403)
                     /gene="mltC"
                     /locus_tag="ACHLCY_003468"
     CDS             complement(6324..7403)
                     /gene="mltC"
                     /locus_tag="ACHLCY_003468"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP0370651.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7468..7743)
                     /gene="yggX"
                     /locus_tag="ACHLCY_003469"
     CDS             complement(7468..7743)
                     /gene="yggX"
                     /locus_tag="ACHLCY_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP0370652.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7771..8823)
                     /gene="mutY"
                     /locus_tag="ACHLCY_003470"
     CDS             complement(7771..8823)
                     /gene="mutY"
                     /locus_tag="ACHLCY_003470"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP0370653.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8984..9703
                     /gene="trmB"
                     /locus_tag="ACHLCY_003471"
     CDS             8984..9703
                     /gene="trmB"
                     /locus_tag="ACHLCY_003471"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP0370654.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9703..10029
                     /gene="yggL"
                     /locus_tag="ACHLCY_003472"
     CDS             9703..10029
                     /gene="yggL"
                     /locus_tag="ACHLCY_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP0370655.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            10213..10932
                     /gene="yggN"
                     /locus_tag="ACHLCY_003473"
     CDS             10213..10932
                     /gene="yggN"
                     /locus_tag="ACHLCY_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP0370656.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            11108..12154
                     /gene="ansB"
                     /locus_tag="ACHLCY_003474"
     CDS             11108..12154
                     /gene="ansB"
                     /locus_tag="ACHLCY_003474"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP0370657.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVAAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12271..13278
                     /gene="yggM"
                     /locus_tag="ACHLCY_003475"
     CDS             12271..13278
                     /gene="yggM"
                     /locus_tag="ACHLCY_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP0370658.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLAMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13334..14635)
                     /locus_tag="ACHLCY_003476"
     CDS             complement(13334..14635)
                     /locus_tag="ACHLCY_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP0370659.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLVYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(14635..15138)
                     /locus_tag="ACHLCY_003477"
     CDS             complement(14635..15138)
                     /locus_tag="ACHLCY_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP0370660.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(15183..16169)
                     /locus_tag="ACHLCY_003478"
     CDS             complement(15183..16169)
                     /locus_tag="ACHLCY_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP0370661.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         4313 a         3898 c         4124 g         4007 t
ORIGIN      
        1 tgcatccaga aaatcggccc accattgtag catcaagcgg cgttcttcca gatgttctgc
       61 tttatgaatg taagcagcac gtacaccgtt acgttcctga tggctcatct gacgttccac
      121 agcatcgcgg gaccacaaac ctgattcaat caatgcactg cacgccatcg cccggaatcc
      181 atggccacag acttcctggg ttgtgtcata ccccatcacc ctcagggctt tatttacagt
      241 attttcactg attggtttac gcgggtcatg tgcacccgta aatatcagac cattttcacc
      301 tgcccatgtc tgaagttctg tcagtatggc tacagcctgt ttgctgagtg gcacaagatg
      361 tggtgttcgc atcttggagc cccggtgtga gaattttacg cctggcagag gttcgcgttc
      421 ggctggaata gtccacatgg cattgtcaat atcaatttca gaccagcggg cgtatctgag
      481 ttcactggaa cgaataaaaa ccagtaaatt cagcataact gccagtcggg tgacaggctg
      541 gcctttataa ccgtcaatac gcgacagaag ttcaggcagg cgtgaaagat ccagagcagg
      601 gcgatgctgg cgttttaccg tggtcaatgc accagccata tccagagccg ggttatatct
      661 gatgatgcca gactgtacag cataacgcat tacagcagat attctttgct gcagacgact
      721 ggcgatttca taaatttgtt tagcttctgc agcacgaaca gggataagca ggtccggggt
      781 attgagtgtt gtgatatccc ggttgccgat aaaggggaaa acgtgtgttt cgaggctttt
      841 gagtacctta tcgctgtgtg attgtgccca ttgtttatta ctggcatgcc agcgtcgtgc
      901 aatatgttca aaagagagtt gtaaatcagg gactactttt ttagctcttt tctgttcgca
      961 agggtcaatc ccctctgaaa tcagttttcg ggcccgtttt gtacgagctt cggagagaga
     1021 aacttctgga tacacaccaa gtgccagagt cttctcctta cccagaatac gataacggag
     1081 acgccagtaa cgggaaccat taggatgaac cagcaggaac atgccagccc cgtcagtgag
     1141 tttataagcc ttgtcagagg gctttgcagc ccggattttt gcgtcagtca gtgccatagt
     1201 acagccttct gaatgcgtta aagagggtac aaattctgtc gaactggggt atacccgcaa
     1261 ttatacccac acaatggggt tgatgtgggg ggaatctggt tgagttcggt agaattgatt
     1321 tgtaggtaga acgcttaaat catggtgttc tgaaacgaaa aacggacctc cgtggaggtc
     1381 cgttgatatg aatttggtgc ccggactcgg aatcgaacca aggacacggg gattttcaat
     1441 cccctgctct accgactgag ctatccgggc aacggggcgc attaaacctg attcgcctcg
     1501 tctcgtcaat caaatttctt caatttactg cagactgcac aatctatcat cactttgccg
     1561 ctattgcacg cattgtcagg caaaccaggc ggtccaggcg gcggaaagcg gcattgccag
     1621 aaggagtgct ggaagcatgt tgaccaccgg gaacatttta atgccacaga tgcgtaatcc
     1681 ggttgccagc agcaataaac cgcctacggc gctgaagtct gccatcatcg acggtgtggt
     1741 cagcggtaat atcagcgcgg catcccacgc cagcgttaac tggatgatca gtaatgggat
     1801 actaattacc gacaccgcaa tacccagcga gcaggcgaag atcatcgccg taaagaaatc
     1861 aagaaatgac ttggcgatta aaatactcgg atcgccggtc atcccttcgt tcatcgcccc
     1921 gaagatcccg gtgccgctgg cgcaaaacag gacaataatc gcgacataat tctgaataaa
     1981 agattcatgc gctggcttct tacgtgagtg acgaaacaga ttttgtgctt tggcgactgc
     2041 tgtattgaca cctttctcca gcaaacaaat ttcgccaatt agagcgccaa gtagcgtagc
     2101 taaaaccatc gctggaaggt tggcgcattt cacaaccagt aaaataccaa tccccagcga
     2161 tgccagacca aaaatagatg tcatggagac gcggatacgt tccggtaagc gttggctgag
     2221 cagtgcgccg agaacgccac ccagtaagac tgcactagcg ttgataaaag ggccgatgac
     2281 cacaagagtt cctgttagct gtattgtttg attttcttat tatgaacttt ttgccttgtt
     2341 gatggcttgt ctggtcgcaa agtcctggct tgcacgcttt agcgaaaggt gccatgattg
     2401 cgcgaatttt ctcctctctg tacggagttt gcccgatgca cgccatctcc ttacattctc
     2461 tcgcttatcg ccgtttcgcg cgaaacgttt ccctttctat gttactgctc atgcggtgaa
     2521 gttaacgcac gctcactgca ggacaacagt aaaatcagag cgtttctgct tttactgatg
     2581 tctggcggtc ggagctggtg accagtttga cccatatctc atggggcagg gttttccacc
     2641 ttgtccggta ttcttacttc cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc
     2701 cgccagtagt gaactggtat cccgactttc ttctcatcgt cgcgtggtgg cgttgggaga
     2761 tactgatttt acggacgtcg cggcagtcgt cattaccgct gcggatagtc gcagtggcat
     2821 tcttgcgttg cttaagcgca ccggttttca tctaccggtg tttttgtatt ccgaacatgc
     2881 tgttgaatta cctgcgggcg ttacggcggt aatcaacggc aacgagcagc agtggctggg
     2941 gctggaatcc gcagcctgtc agtatgaaga gaatttgctg ccaccgtttt atgacacgct
     3001 gacgcagtac gttgagatgg gcaacagcac ctttgcttgc cctggacatc aacatggtgc
     3061 gttttttaaa aagcatcctg ccggacgcca tttttacgat ttctttggtg agaacgtctt
     3121 tcgcgccgat atgtgtaacg ctgacgtaaa attgggcgat ctgcttattc atgaaggatc
     3181 ggcgaaagat gcgcagaaat tcgcagccaa agtctttcat gccgataaaa cctattttgt
     3241 gctgaacggc acatcggcag cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga
     3301 tctggtgctc ttcgaccgta acaaccataa gtcgaatcat cacggcgcgc tgattcaggc
     3361 gggggcgacg ccggtctatc tggaagcttc acgcaacccg tttggtttca ttggcggtat
     3421 tgatgcgcac tgttttaatg aagagtatct gcgccagcaa attcgcgacg ttgcgccaga
     3481 aaaagccgag ctgccgcgcc cgtttcgcct ggcgattatt cagctgggaa cctatgacgg
     3541 aactgtctat aacgcccgtc aggtgatcga taccgttggg catctgtgtg attacattct
     3601 gtttgattcc gcgtgggtcg gttacgaaca atttatcccg atgatggcgg atagctcgcc
     3661 gctgctgtta gaacttaacg aaaacgatcc ggggattttt gtgacccagt cggtgcacaa
     3721 acagcaggct ggattctcac agacgtcgca gatccataaa aaagataacc atatccgcgg
     3781 acaggcgcgt ttttgcccgc ataagcggtt gaataacgcc tttatgctcc atgcttctac
     3841 cagccctttc tatccgctgt ttgctgcact ggatgttaac gccaaaattc atgaagggga
     3901 gagtgggcgt cggctgtggg ctgagtgcgt tgagttgggt attgagtcgc gcaaggctat
     3961 tcttgcgcgc tgtaagctgt tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg
     4021 gcaggattat ccgacatctg tgttagccag cgaccgccgt tttttcagtt ttgagccggg
     4081 ggcgaagtgg cacggctttg aaggatatgc cgcggatcag tattttgttg atccgtgcaa
     4141 gctgttactc actacaccag gtatcaatgc cgaaactggc gaatatagcg actttggcgt
     4201 tccggcgacg attctggcgc actatctgcg tgagaacggc attgtgccgg agaagtgcga
     4261 tctcaactcc attctgttct tattaactcc ggcggaaagc cacgagaagc tggcacaact
     4321 ggtggcgatg ctggcgcaat ttgaacagca tattgaggat gactcgccgc tggctgaggt
     4381 gttgccgagc gtttataaca aatatccggt gcgctatcgc gactacaccc tgcgccagtt
     4441 gtgtcaggag atgcacgatc tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat
     4501 gttccgccag cagagtttcc cgtcagtggt gatgaacccc caggatgcgc atagcgctta
     4561 tattcgcggt gaagtggagt tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga
     4621 aggggcgttg ccttatccac ctggcgtgct ttgcgtggta cccggggaag tctggggtgg
     4681 ggcggttcaa cgttatttcc ttgcactgga agaaggggtg aatttgttgc cgggattttc
     4741 gccggagctg caaggtgttt atagcgaaac cgatgcggat ggcatgaaac ggttgtacgg
     4801 ttatgtgttg aagtaagaat aaaaaaaacg ggtcaccttc tggcgacccg tttttctttg
     4861 cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt atatttgaac
     4921 atcgccatga acgcgaaggc cagaaccacg gagtaaccag cgaaaatcaa ccatacggtc
     4981 tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct cacgatgccg
     5041 ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc actggcgcga
     5101 attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa gaagtcgaat
     5161 gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg agtcgggtcg
     5221 ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat cattacgttc
     5281 ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt ttcagagatc
     5341 tgcgaaatcg acatgatgat tgacgcatgc tgcacaataa agctgctggc aaacatcgga
     5401 tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg cagttccgcg
     5461 cccagcagca ttgagaagat aaagaagatt gccatacgct tgtttttaaa cagcgcgaat
     5521 gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc aaccggaata
     5581 tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gtgctgcgcc aatatacagc
     5641 tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat gataaagccg
     5701 atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc ggcattttgc
     5761 aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc aaacgagtta
     5821 atcaatatca caaggaacat cgcctccggt gtcgtgacct gtgccgccat gaacagcgtg
     5881 atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca tttgtcggcc
     5941 acaatcccca gcagcgcagg cataaagacc gcggcgatac ccagtgagct ataaactgcg
     6001 ccaatagaag caccgtcaaa cttcagggta acaaacatat aggagccgag ggtcgtcagc
     6061 caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag cttaagattc
     6121 atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat ttgaccgtgc
     6181 gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg acaaataatt
     6241 gcaaacattc ccaatttgca aaacgaaatt gtgatgtgga taacattttt gcccctgagc
     6301 atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc ggtattcact
     6361 ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt ctgataaaca
     6421 tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt cgaaaagact
     6481 cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg ccgcgatgtt
     6541 gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc ggtgccggta
     6601 tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga tttcccctgc
     6661 gagcggaaca catctttccc ggcagtatgt tgtaccacct gcattaatcc cagcgcatcg
     6721 gaacggctga ccgcatatgg gttaaaggaa gattcggtct gcataatcgc cagaatcagc
     6781 gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata tttgtgcgca
     6841 cgtttatcaa ggtggttcgg caccatgtta atggtaacgc tgtagatgat acgcagcccg
     6901 ttgctgcggc tcttcagacg gtttttcagc agataatccg cgaagttgct cgcgcgacct
     6961 tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa aggttctttc
     7021 gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc gtcacccatc
     7081 agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc ggcgatagtt
     7141 tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata ttgatcggtg
     7201 tatttcacgt agtccttagg accggcgatc accacctctt tgaatcccca gatgttctca
     7261 atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt gacccaggct
     7321 tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg cgcaatcaaa
     7381 gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt ttatgcaagt
     7441 tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat agccctcgat
     7501 atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctccagca gcttgcggtg
     7561 ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgtt tgtgctgcca
     7621 ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct cgccggggta
     7681 cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct
     7741 cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg
     7801 taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta agttatacca
     7861 gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc acataggcac
     7921 aatatctaag tggaaatggc tgaaggtatg ccgaaacgcg gtcagctgcg tcaggttatc
     7981 ggcagcaatc tgccgttgcg ccagccactg ccgcaaactt tcttcgtcgg caaactgcgg
     8041 gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca atacttcatc
     8101 ttcgtgctgt aatagcaaaa agtagccggt gcgctccggc agcgtctgtt tcggtttttt
     8161 gcccggataa agcgcccagc tattgttggc ggcggcaata catccgtttt gtagcggaca
     8221 gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca cccaaatcca tcatcgcctg
     8281 attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc ataatttatt
     8341 ctcgacctct tttttccctg gccagccgct tacagcatag cagcgcgcca gcacccgttt
     8401 gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa tcgcacctgc
     8461 ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg ggaatttacc
     8521 gccgtgtaag gtcgccactt gttgtgccgc tttatgcagg ttgcgcgcgc gggcgtaata
     8581 gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga gatcggtcac
     8641 cgtcgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa cctgagtttg
     8701 ttgcaacatc acttctgaga gccatacttt gtagggcgtc ttgtcaattt gccagggcag
     8761 cgttttccgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg
     8821 catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac ggcagggtgt
     8881 aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac taactttgga
     8941 taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa acgacgtcat
     9001 ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt ttgtgcgccg
     9061 ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc cggtgatggg
     9121 cgttgagttc agcgaagata tgctggattt ccccgcgctt tttggccgtg aagcgccggt
     9181 gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta aagatcgccc
     9241 tgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt gcctggcttc
     9301 tgcgcatgaa gaaggtttaa gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct
     9361 gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc ctgacccgtg
     9421 gcacaaagcg cgccataata aacgccgtat cgtacaggtg ccgtttgccg aactggtaaa
     9481 aagcaaactg cagctggggg gcgtattcca tatggcgacc gactgggaac cttatgcgga
     9541 acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag agagcaatga
     9601 ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg gtcatcgtct
     9661 tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa gaaccgtagc
     9721 cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt ttcggtggca
     9781 tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga tgattttatt
     9841 aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct ggcctgggaa
     9901 ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt
     9961 aagtggctgg aagagcgcaa actggatgag gtacgcacca gcgaactttt cgacgtttgg
    10021 tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc gctacgctta
    10081 tcaggcctgg aaagatgcac gatcgagtag gcgggataag gtgtttacgc cgcatccggc
    10141 atggaaaacg cgtactttgt tatcaatctg gggccagcaa atgctggcct gattttttct
    10201 tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt gacggcaatg
    10261 accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg
    10321 caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga cggcaacgtg
    10381 atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc gaaggattat
    10441 caggctgaac tacgcagcac gctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgaa
    10501 aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag cagcaaaatg
    10561 cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg cattatcgaa
    10621 acgcgcagcg atggcctgac gtttcactat aaagccattg atcaggttcg cgccgaaggc
    10681 cagcaattag tgaatcaggc aatgggtgga attttacagg acagcattaa tgaaatgggc
    10741 gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg aagcctgggg
    10801 gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt
    10861 ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc cctggtcggg
    10921 aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg tttaattctt
    10981 cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt tgtaaattgt
    11041 ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgttcac gtaactggag
    11101 gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg gttttagtgg
    11161 tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca ttgccggtgg
    11221 tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag aaaatctggt
    11281 taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg tagtgaatat
    11341 cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa ttaacaccga
    11401 ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg aagaaaccgc
    11461 ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg gcgcaatgcg
    11521 cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg tagtggccgc
    11581 agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca ccgtactgga
    11641 cggtcgcgat gtcaccaaaa ccaacaccac cgacgtagcg accttcaagt ctgttaacta
    11701 cggtcctctg ggatacattc acaacggtaa gattgactac caacgtaccc cggcacgtaa
    11761 gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga aagtcggcat
    11821 cgtttataac tacgctaacg catccgatct tccggctaaa gcactggtag atgcgggcta
    11881 tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg tgttcgacac
    11941 cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg taccgacggg
    12001 tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg cctctggcac
    12061 gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa ccaaagatcc
    12121 gcagcagatc cagcagatct tcaatcagta ctaatcacct cgccccggta tcgtgccggg
    12181 gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg ataatcaccg
    12241 gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc aatggattgt tggcacggca
    12301 ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac tgaaaatatc
    12361 ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc tatcacctta
    12421 aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga tgtctcttcc
    12481 agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct cgaacagacc
    12541 tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg
    12601 tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg tgggcgggtg
    12661 gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga tttcaattat
    12721 cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc tgcgctggaa
    12781 aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc ggatgcatat
    12841 tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa aaaaattcac
    12901 caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt taaatatgac
    12961 aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga gtgttatgaa
    13021 gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca gacttttctg
    13081 caatcacaaa aactcagccg taaattgcag gatgactggg taacgcttga aaaagggcaa
    13141 tatccgttag cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat tttgatgaaa
    13201 attgacgata tcaaccaagc taatgaacgc tggaaaaaag ataccgaaca actgcggcgc
    13261 aatggggtga ttaagtaagt ttgatatagt gcctttgccg gataatataa aaagttatcc
    13321 ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat atttgcggaa
    13381 tgtaagtgat catcatcaac gctactagca atgcagcata aaatggcagc aatggtttga
    13441 ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca ctacccaccg
    13501 gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa tgtactgggt
    13561 ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt acagccggtg
    13621 tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc aatatgatca
    13681 gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata tcagcattag
    13741 tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc gaagtggtaa
    13801 caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg taaaccagca
    13861 ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg gtaaaaatgc
    13921 cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag gcatccagaa
    13981 acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca cggcgtttag
    14041 caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc aaatatcccg
    14101 ccataaatag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca attaacacgt
    14161 ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg gtagaaaatg
    14221 ccggatcata accttgtttg gtctgaatgg gattcagtgt gccaccaacg gcagcagctg
    14281 ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg ttcacatgac
    14341 caagtgaacc aggaacccga ccaaccatta cctgggcgag gttaatcaaa cgtatcgcaa
    14401 taccgccgct gttcatcagc gttccggcaa agatgaaaaa ggggattgcc aatagggcaa
    14461 agttatccag tccattagcc agtcgctgcg aaacagtgat tgccgcgata tcccagggaa
    14521 acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg ggcacaccaa
    14581 taaagaccaa cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta tccattaact
    14641 ggcctcctgc gcatgttgtt tcagttgttg aatgctttga ttaataaaac acaaggcata
    14701 aaacatcatc accaatccac ttaatggcag aatgatatat acgtatccca tcggtatttg
    14761 catcgcggca gatacctgtg atgttgccag cgttttatca attaatttca ggccaccagt
    14821 cacaatgacc gaaccggcaa aacctaaaat aagaacgtta atgactatac taagaagtaa
    14881 ttgcttgcgc ttattcaggg cgagagcaaa taaatcgata gagagatgtc tctgcgcgcc
    14941 gacggtatat gcagcaccta ataaccctac ccagatcatc aaaaatcgtg ccagctcatc
    15001 ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga ctacgcatac
    15061 aaccagagca agcatgacgg cgacagaaaa cgcggcaatg acgcggtcta ccgcaagttt
    15121 caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagagac tggcccggaa
    15181 tattattgag cagcgttgtc gaacttctct aacaaagcag cttgttttgg atcttttttg
    15241 aagtcgtcaa acagaggctg aactgcggca cggaatggtg ctttatccac ttttacaatt
    15301 tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc ccacaatttt
    15361 tgttgataag cttcggattt cttcgctgct gtttcgagga tttgttgctg ttctggggtt
    15421 aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc gtgttcatct
    15481 tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca cccaggaagg cacgttattt
    15541 tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa tgaaattgga
    15601 gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg ctgaacacga
    15661 attttcagcc ctttaagatc ttctggctta gtaatcggtt ttttcgcata gaaactgcgt
    15721 gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga gtccataatt
    15781 tctttaccaa cttcaccaaa aacaactttg ttgaagtgag cctgatcttt aaataagaac
    15841 ggcaaattat agatagcata aatattatca aaagactcca gatcactggc ggaacctttg
    15901 gtcatatcca gagcgccatt ttgcaacaac tccatagttt ctcgcgcatt acccatctgg
    15961 ctgctgggat aaatacggat caccatatta ccttttgaca gctgttttac ctctttcgcc
    16021 agctcttcaa aactctgatg tacaacatga cttctttcca ggttgtgtgc taactttaac
    16081 gtcacttttt ctgcggcgtt tacgccagtg ctaaccagag ccagcaagga tgcgcagact
    16141 aatgagcggg taaatgacct cattttcata aaaaaactcc ctgttgaagg atggaaattg
    16201 gttgatatct aattgtgctt atcagtgctt tgtgcgacaa attggttaac ctgtctgaca
    16261 acttgtctat gtttatcaca acttgaaata aagtcagaaa ttaaatatta aatttgtgcg
    16321 ccaggttgta catgtttata at
//
LOCUS       DAZGTR010000095        16283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGTR010000095 DAZGTR010000000
VERSION     DAZGTR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.095"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..4053
                     /locus_tag="ACHLCY_003479"
                     /pseudo
     CDS             1..4053
                     /locus_tag="ACHLCY_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
     gene            4276..4542
                     /gene="hipB"
                     /locus_tag="ACHLCY_003480"
     CDS             4276..4542
                     /gene="hipB"
                     /locus_tag="ACHLCY_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP0370662.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4542..5864
                     /gene="hipA"
                     /locus_tag="ACHLCY_003481"
     CDS             4542..5864
                     /gene="hipA"
                     /locus_tag="ACHLCY_003481"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP0370663.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6176..6384
                     /locus_tag="ACHLCY_003482"
                     /pseudo
     CDS             6176..6384
                     /locus_tag="ACHLCY_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6816..7379
                     /gene="fimA"
                     /locus_tag="ACHLCY_003483"
     CDS             6816..7379
                     /gene="fimA"
                     /locus_tag="ACHLCY_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0370664.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            7740..8450
                     /locus_tag="ACHLCY_003484"
     CDS             7740..8450
                     /locus_tag="ACHLCY_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0370665.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            8492..11143
                     /locus_tag="ACHLCY_003485"
     CDS             8492..11143
                     /locus_tag="ACHLCY_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0370666.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTIFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVITVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            11157..11687
                     /gene="ydeS"
                     /locus_tag="ACHLCY_003486"
     CDS             11157..11687
                     /gene="ydeS"
                     /locus_tag="ACHLCY_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0370667.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            11700..12203
                     /gene="ydeR"
                     /locus_tag="ACHLCY_003487"
     CDS             11700..12203
                     /gene="ydeR"
                     /locus_tag="ACHLCY_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0370668.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            12262..13176
                     /gene="ydeQ"
                     /locus_tag="ACHLCY_003488"
     CDS             12262..13176
                     /gene="ydeQ"
                     /locus_tag="ACHLCY_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0370669.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            13510..15789
                     /gene="ydeP"
                     /locus_tag="ACHLCY_003489"
     CDS             13510..15789
                     /gene="ydeP"
                     /locus_tag="ACHLCY_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP0370670.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMSDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            16037..16234
                     /gene="safA"
                     /locus_tag="ACHLCY_003490"
     CDS             16037..16234
                     /gene="safA"
                     /locus_tag="ACHLCY_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP0370671.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT         4555 a         3547 c         3992 g         4189 t
ORIGIN      
        1 cgtcggaaac ctcaatatta gcgatggcgg taagttcgta tcgcaaaata ttactttttt
       61 gggcgataaa gcaagcggta tcggcacact gaacctgatg gatgcgacat catcgttcga
      121 tactgtgggt atcaatgtcg gtaattttgg tagcggtatc gtaaatgtca gtaatggtgc
      181 cacccttaat tcaacgggct atggatttat cggaggaaat gcctccggta aggggatagt
      241 taatatttca acggacagtc tctggaattt aaagacatca tctactaacg cacaattgct
      301 acaggtcggt gtattaggca agggtgaact gaatattacc accggaggta tagttaaagc
      361 gcgtgataca cagatagctc tcaatgacaa aagtaagggc gacgtgaggg tggatgggca
      421 gaactctctt cttgaaacat tcaatatgta cgtagggaca tctggtacgg gtacgttaac
      481 cctgacgaat agcggcacgc tgaatgtcga aggtggagaa gtttacttag gtgtttttga
      541 acctgctgta ggaacgctaa acattggtgc agctcacggt gaggcggcgg cagatgccgg
      601 gtatataacc aacgcgacga aagtagagtt tggttctggc gaaggtgttt ttgtctttaa
      661 tcatactaat aacagtgatg ccggctacca ggtcgatatg ctgattacag gtgacgataa
      721 agacggaaaa gtgatccatg atgcaggcca tacggtgttc aatgcaggga atacttatag
      781 cggtaaaacg ctggtcaatg acggcctcct gaccattgcg tctcatacgg cagatggggt
      841 aacgggcatg ggttcgagtg aagtcaccat tgcaagcccc ggtacgctcg acattctcgc
      901 atcaacgaac agtgcaggag attacacgct gaccaatgcg ctcaaaggcg atggcttgat
      961 gcgagtgcag ctgtcatcct acgacaagat gtttggcttt acccatgcaa cagggactga
     1021 attcgccggt gttgcccaac tgaaagacag taccttcact ctggaacgcg acaacaccgc
     1081 tgcgcttact cacgcgatgt tgcagtctga cagtgaaaat accacatcgg taaaagtggg
     1141 agagcaatcc attggtggac tggccatgaa tggcggtacg ctcattttcg atacggatat
     1201 tcctgctgcg acgcttgcag agggatatat cagcgtcgat acgctggttg tcggcgcggg
     1261 tgactacacc tggaaaggcc gtaactatca ggtaaacggg acgggcgacg tgcttatcga
     1321 cgtgcctaaa ccgtggaatg atcccatggc gaataaccct ctgacgacgc tcaatttgct
     1381 ggaacacgat gatagccatg tcggcgttca actggtgaag gcgcaaacgg ttattgggtc
     1441 gggtggctca ttaacgttac gtgatttaca gggcgacgag gtggaagcgg acaaaacgtt
     1501 acacattgcg caaaacggaa cggtggtcgc cgagggtgat tatggattcc gcctcacgac
     1561 cgcaccaggt gatggtttgt acgttaacta tgggctgaaa gcgctgaaca tccatggtgg
     1621 gcaaaagctg acgttagccg aacatggcgg agcctatggc gcaacggccg atatgtcggc
     1681 aaaaatcggt ggtgaagggg atctggcaat caatacggtg cgacaggttt cgctttccaa
     1741 cggtcagaac gactatcagg gggcaaccta cgttcagatg gggacattac gtaccgatgc
     1801 ggatggtgca cttggcaaca cccgggaact gaacatcagc aacgcggcca tcgtcgatct
     1861 taatggatcg acgcagacgg tagagacatt caccgggcag atgggttcga ctgttttgtt
     1921 caaagagggg gcgctgacgg taaataaagg tgggatcagt cagggtgaac tgacaggtgg
     1981 cggaaacctg aatgttacag ggggaacgct ggctatcgag gggcttaatg cacgctacaa
     2041 tgcgttaacc agcattagcc caaatgcgga agtcagcctc gataatacgc aggggttagg
     2101 cagaggaaat attgccaata acggtctgtt aacgctaaaa aacgtgactg gcgaactgcg
     2161 caatagcata agcgggaagg gtatcgtgag cgcaaccgcc aggacagatg tagagctgga
     2221 tggcgataat agccgctttg tggggcaatt caacattgat acaggcagcg cgctcagcgt
     2281 caacgagcag aaaaacctgg gtgatgcttc cgttatcaat aatggcctgc tcaccatctc
     2341 cactgagcgt agctgggcga tgacgcacag tatcagcggt agcggtgatg tgacaaaact
     2401 gggtaccggg atcctgactc ttaacaacga ttccgcggcg tatcagggta cgacggatat
     2461 cgtggggggg gaaattgctt tcggttccga ctctgccatt aatatggcaa gtcaacacat
     2521 taatatccat aacagcggtg tgatgtcggg aaatgtcacc actgcaggtg atgtgaacgt
     2581 tatgcctggg gggacactgc gtgtcgctaa aaccacaatc ggcggcaacc tggagaatgg
     2641 cggcacggtt caaatgaata gcgaaggggg aaaccgggga atgtactgac cgttaacggc
     2701 aactataccg gaaacaatgg cctgatgacg ttcaacgcga cgctgggcgg cgataattcg
     2761 cccaccgata aaatgaacgt gaaaggcgat acccaaggga acactcgcgt tcgggttgat
     2821 aacattggcg gcgtcggtgc acaaacggtc aacggtattg aactcattga ggttggcggt
     2881 aattctgcag gtaacttcgc gctgaccacc ggaactgtcg aagctggggc ttacgtctac
     2941 acgctggcta aagggaaggg gaatgacgag aaaaactggt atctgaccag taaatgggac
     3001 ggcgtaacgc cagcggatac acccgatccc atcaataatc cccctgttgt ggatccggaa
     3061 agcccatcag tttatcgccc ggaggccgga agctatatca gcaacattgc cgcagccaac
     3121 tcgctgttta gccatcgttt acacgaccgt ctgggtgagc cgcagtatat agattcactg
     3181 cattctcagg ggtcggcaag cagtatgtgg atgcgtcatg tcggagggca cgaacgttca
     3241 agggccggtg acggtcagct aattactcag gctaaccgct atgtattgca gctaggcggc
     3301 gatttggcgc agtggagtag caacgcgcag gatcgctggc atcttggcgt gatggcaggc
     3361 tacgccaatc agcacagtaa tactcagagt aatcgtgtgg gttataaatc ggatgggcgc
     3421 atcagcggtt acagcgctgg gctgtacgcg acctggtatc agaacgatgc gaataagacc
     3481 ggcgcttatc ttgacagctg ggcgctgtat aactggtttg ataacagcgt cagttccgat
     3541 aaccgttctg ctgacgacta tgattctcgc ggtgtgacgg cctctgttga gggtgggtat
     3601 acctttgaag cgggaacatt tagaggcagc gaagggacgc tgaatacctg gtacgtccag
     3661 ccacaggcgc aaatcacctg gatgggtgtg aaagattctg accataatcg gaaagacgga
     3721 acacgcatta aaacggaagg cgacggaaac gtgcaaacgc gacttggggt gaaaacctac
     3781 ctgaatagcc atcaccagcg tgacgatggt aaacagcgtg agttccagcc ttacattgaa
     3841 gcgaactgga tcaacaatag caaagtctac gccgtgaaga tgaatggtca aaccgtaagc
     3901 cgtgatggtg cgcgtaatct cggtgaagta cgtaccgggg ttgaggcgaa agtaaataac
     3961 aaccttagcc tgtgggggaa tgtcggtgtg caactaggtg ataaaggcta tagcgatact
     4021 cagggcatgc tgggagtgaa atatagctgg taaaccgtat aagccgcatg tcgagatggc
     4081 atgcggctta atattgccga cttcaaacgg cgcatcagcg ccttttttaa atcctccttt
     4141 ttatccgcga tcgcggatat cgcagcgttt atcccgtaga gcggataaga cgtgtttcca
     4201 aattgactta tcctcactaa aggataaaac tgataatatc ctcttatgcg gataaatttg
     4261 ctgtggacgt atgacatgat gagctttcag aagatctata gcccaacgca attggcgaat
     4321 gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga gcgagctggc gaaaaaaatt
     4381 ggcattaagc aggcgacaat ttccaatttc gaaaacaacc ctgacaatac cacgctcacg
     4441 acatttttta agattttaca gtcgcttgaa ctctcaatga cactatgcga cacgaaaaat
     4501 gcctcgccag aatcaacaga acagcaagat ctggagtggt aatgcctaaa cttgtcactt
     4561 ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc caacggcgcg cacaccttta
     4621 agtatgcacc ggagtggtta gccagccgtt atgccagacc gttgtcactt tcgctgccat
     4681 tgcagagggg gaatatcacc tcagatgccg tatttaactt cttcgataac ctgttacccg
     4741 atagcccgat tgtacgtgac cggatcgtta aacgttatca tgctaaatcc agacaaccgt
     4801 ttgatttatt gtcagaaata gggcgagaca gcgttggtgc cgtaacgtta ttacccgaaa
     4861 atgaaaccat aacgcgcccg ataatggcat gggaaaagct tactgaagcc aggcttgaag
     4921 aagtattaac ggcttataaa gcagatatcc ctctgggcat gattagagaa gaaaatgact
     4981 ttcgcatctc ggttgctggc gcgcaggaga agacagcgct gcttagaata ggcaatgact
     5041 ggtgcattcc gaaaggaata acgccgacga cgcacatcat taaattaccg attggcgaaa
     5101 tcagacagcc caatgcgacg ctcgatctca gccaaagcgt tgataatgag tattactgtc
     5161 tgctgctggc gaaagaactt gggttgaatg ttccggacgc agaaatcatt aaagcgggaa
     5221 atgtgcgcgc gttagcggtc gaacgttttg acaggcgttg gaatactgag cgaacggttt
     5281 tacttcgctt gccacaggag gatatgtgtc agacattcgg tttaccttca tctgtgaaat
     5341 atgaatcaga tggaggccca ggcatcgcgc ggatcatggc gtttttgatg gggtccagcg
     5401 aggcgcttcg cgatcgttat gattttatga aattccaggt cttccagtgg ttgattggcg
     5461 caacggatgg ccatgcaaaa aacttctccg tatttattca ggctggcggc agttatcgac
     5521 tcacgccatt ttacgacatc atttcagcat ttccggtcct tggcggtacg ggaatacaca
     5581 tcagcgatct caaactggca atggggctta acgcatccaa aggcaaaaaa acggcaatcg
     5641 ataaaattta tccgcgacat tttttggcga cagcaaaggt gctgagattc ccggaagtgc
     5701 agatgcatga aatcctaagt gactttgcca gaatgattcc ggcagcactg gataacgtga
     5761 agacttcatt accgacagat tttccggaga acgtggtgac ggcagttgaa accaatgtgt
     5821 tgaggttgca tggacggtta agccgagaat acggtagtaa gtgagatatg gggtttggtc
     5881 gttgttaatg agcacgacaa tcatgaccgc caacgactgg attcttcctt ggtttatact
     5941 ggtgcgcaca atgtatatta tgttaaatca tgtctgagtg atgcgaagtg cttttctggt
     6001 agtcgttatt cgttcagggt tcaagaactt aagtctctcc tctttcataa ttggcctgtg
     6061 catggagaaa tagcctggtt attccctgcc ccatgctaag agcgcagtgt ctgtcgcata
     6121 gacactgcat acgaaaacac cagagggtgc tgttacttat ttaaggacgg atcaagcggg
     6181 aatgagtcca ctccggtatc agaaatggct acgtctcaat gaagtcaggc gaccgatgct
     6241 gaatgaacat tacgatgtca ccacagcagc ctatgctgtc ggttacgaaa gctatcccat
     6301 ttcagtcggg aatattcgcg gatgtttgga gagtcaccca agagagatat taccaggtta
     6361 agaaagtctg tcggtcagct ttaataaacc ctgcacttat ctgtttagag caattcggtg
     6421 ttagtttcag caagcaaaca ttaaccatag ctaatgattt atagccatat taaccattgg
     6481 tagcccttga cctttcctga cacttccctt gttcgcaatg atattctgtt tagtcacggt
     6541 ttataaataa catgcataat gaaatcgtca tgttatttag cgacataaaa tgtttttgca
     6601 aatactctct tcaacaagtg cacaataaac ttgttgtgat taggtgtcct tgtgatttaa
     6661 agatattatc gttcttcgtg aaatgtaata accctggaaa ctatattctc agggcaacaa
     6721 ccaatcaaga atgattattg tttttaagca ataacagaat gacttaattg ttaaccctct
     6781 ttattcagct acttaaatat aaattttgga gaattatgaa actcaaacat gttggtatga
     6841 ttgtcgtttc tgtgttggcg atgtcgtctg ctgcggtaag cgcagccgag ggtgatgaat
     6901 cagtaacgac cactgttaat ggcggtgtta ttcattttaa aggtgaagtg gtaaatgccg
     6961 cttgtgcaat tgattcagaa tcaatgaacc aaacggttga gctgggtcag gttcgttctt
     7021 ctcgcctggc taaagcgggt gacctcagct ccgccgttgg cttcaatatc aagctgaatg
     7081 attgtgatac caatgtttcc agtaatgcag ctgttgcatt cctgggtact actgtcacca
     7141 gtaatgacga tacgttagcg ctgcagagtt cagcggcagg ctctgcccaa aatgtcggta
     7201 ttcaaatttt ggaccgtacg ggtgaggtat taatacttga tggggccact tttagtgcta
     7261 aaaccgactt gattgatggc acgaatatac tgccattcca ggctcgttat attgctctcg
     7321 ggcagtccgt agctggtact gcaaacgctg atgcgacctt caaagttcaa tatctataat
     7381 tcaatcttaa ggtggctata cgtcatctga aattgaaaca gggtgtgtgt ggcatggcta
     7441 cgcacacccg agcaatttac caatattcat ctcattttta atgagaaatg gtttgtattt
     7501 ggagcgctat gaaaaatcac ataatatcta aactatcaat aaacaacagt agttaaactt
     7561 actgttattt aaaatattta ctttgtagca aggttttcct attaaaagag aaccaacatt
     7621 tattacaaac gcattaagtc tggaaacccg gcgactcgcc aatgtattag caacattaat
     7681 cgattgtctg atgtaacggt tttcccgctt atcctcttaa cacatggaga atgcatcaga
     7741 tgcaaactac gcgtaccccc tactctattt cctttatggc gacagtgtta cttttattgc
     7801 tctttgcttg ccatagcacc gttgccaacg cagctgtcgc actgggtgcg acgcgggtaa
     7861 tttatccggc aaaccagaaa caggttttat tgcctgtcac gaataatgat cctgccagtg
     7921 tctatctcat tcagtcatgg atagaaaatg caggtgacca aaaagacacc cagtttgtca
     7981 ttaccccacc gcttttttcc atgcaaggta aaaaagagaa tacgctgcgg ataatcaacg
     8041 ctactaatca tcagttacca ggcgaccgtg aaagcctgtt ctgggtgaat gttaaagcta
     8101 ttcctgctat ggaaaaagac cagaagaatg agaatacatt acagctggca attattagcc
     8161 gcataaaaat gttttatcgc cccacgaacc tggcaatggc acctgaagaa gcgcctgcaa
     8221 tgcttcggtt tcgtcgcagc ggtagcaaac tcacgcttat taatccaacg ccctatttca
     8281 ttaccgtaac aaatatgaaa gcaggaaata gcaatctccc aaacaccatg gtgccgccaa
     8341 aaggtgaagt atcggtggat atcacgcatg ctgcaaccgg ggatatcagt tttcagacga
     8401 tcaatgatta cggtgcatta actccgcgta ttaaggcaac catgcagtaa gccagtgacc
     8461 taattaataa taaactcaat cggaattaat aatgacagcc tttcgtgccg catttaaggc
     8521 atatcgtatg catcaggtgt taataatgcc tcgctttgca cggcttacca ttgcccttgg
     8581 cttagcaact gcggtatttc ccgttgacgc agagtattat tttaatccac ggtttttaag
     8641 caatgatctt gctgaatctg tcgatttatc cgcctttact aagggacgcg aggcaccacc
     8701 tggtacatat cgggtcgaca tttatttgaa cgacgaattt atgactagtc gggatattac
     8761 ttttattgca gatgataata acgcagaact catcccatgc ctgagtacag atcttcttgt
     8821 cagtcttggt ataaaaaaaa tagcgttatt agacaataaa gaacattcgg cagaaaaaca
     8881 tgtgccggac aacagcgcat gcacaccact tcaggatcgt ttggccgacg catcaactga
     8941 gtttgatgtt ggtcagcaac atttgtcact ctccgttccg caaatttatg ttggcagaat
     9001 ggctcgcggc tatgtttctc ctgatctgtg ggaagaagga ataaatgctg ggcttctaaa
     9061 ctatagtttt aatggtaatt ctattaataa tcgtagtaac cataatgcag gaaaatccaa
     9121 ctatgcatat ttgaatttac agagtggcat caacattggt agttggcgac tgcgcgataa
     9181 ctcaacgtgg agttataaca gtgggagtag caattcatct gacagcaata aatggcagca
     9241 tatcaatacg tcggctgaac gtgacattat tcccttacgc tcacggttaa cggtaggtga
     9301 tagttatacc gatggcgata tttttgatag tgtgaacttt cgtggcctca aaataaattc
     9361 aacagaagcg atgttgcccg atagccaaca tggtttcgct ccggtgattc atggtattgc
     9421 ccgtggaacc gcacaagtga gtgtaaaaca gaacggatac gatgtttatc agactactgt
     9481 tccacccggc ccttttacta ttgatgatat caactctgcg gccaatggtg gtgatctgca
     9541 agtaaccata aaagaggcag acggcagtat tcagacatta tatgttcctt attcgtctgt
     9601 tccggttctc caacgtgctg gatatacgcg ttatgcgctt gccatggggg aatatcgtag
     9661 tggaaataac ctgcaaagct ctcccaagtt catacaaggt agcttgatgc atggtctgga
     9721 aggaaactgg acaccttatg gcggaatgca aattgcagaa gattatcagg ccttcaacct
     9781 tggtattggg aaagatttag gactttttgg tgccttttct ttcgatatca cgcaggccaa
     9841 tacgacactt gcagatggca cccgtcacag tgggcaatcg gttaaatccg tctacagcaa
     9901 atccttctac cagacaggaa ccaatatcca ggtcgcagga tatcgctatt ctacgcaagg
     9961 tttttataac ttatccgaca gtgcctacag tcgaatgagt ggttacaccg tcaagcctcc
    10021 taccggagac accaatgagc agacacaatt tattgattat tttaatctgt tctacagtaa
    10081 gcgtggtcag gaacaaataa gcatctctca gcagcttgga aattacggta cgatattttt
    10141 cagtgccagt cgccaaagtt actggaacac gtcacgcagc gaccagcaaa tatcatttgg
    10201 attaaatgtg ccgtttggtg atattacgac ttcgctgaat tacagctatt ccaataatat
    10261 atggcaaaac gatcgggatc atttactcgc ttttacgctt aatgttccct tcagtcattg
    10321 gatgcgtaca gacagtcagt cggcatttcg taattcaaac gccagttaca gtatgtcaaa
    10381 cgatttgaaa ggcggcatga ccaatctatc gggggtttat ggcactctgc tgccggataa
    10441 taacctgaat tatagcgttc aggtcggtaa cacccacgga ggtaatacat cgtctggcac
    10501 cagtggttac agttctctta attatcgtgg agcttatggt aatactaatg tcggttacag
    10561 tcggagtggt gacagcagcc agatttatta cggaatgagt ggtgggatta ttgctcatgc
    10621 tgatggcatc acctttggac agccgctggg cgacacaatg gttctggtta aggctcctgg
    10681 tgctgataat gtcaaaatag agaaccagac cggaattcat accgactggc gtggctatgc
    10741 catattacca tttgcgacag aatatagaga aaaccgtgtt gctcttaacg cgaattccct
    10801 tgcagataat gttgaactgg atgaaaccgt gatcactgtc atcccaactc acggtgctat
    10861 tgccagagca acatttaatg cacaaatcgg cgggaaagta ttaatgacgt tgaagtacgg
    10921 taataagagt gttccattcg gtgcaattgt cacacacgga gagaataaaa atggcagcat
    10981 tgtcgcagaa aacggtcagg tttatctgac tggacttcca cagtcaggga aattacaggt
    11041 ttcatggggc aaagataaaa actcaaactg tattgtcgag tacaagcttc ctgaagtttc
    11101 tcctggtacc ttactgaacc agcagacagc aatctgtcgc taaggaacaa tagattatga
    11161 aatacaataa cattattttc ctcggtttat gtctggggtt aaccacctat tctgctttat
    11221 ccgcagatag cgttattaaa attagcgggc gcgtcctcga ttatggctgc acagtctcat
    11281 cggattcgct taattttacc gtagatctcc aaaaaaacag tgccagacaa tttccaacga
    11341 ccggtagcac aagtccagcc gtcccttttc agattacgtt aagtgaatgc agcaaaggga
    11401 caacgggggt tcgggttgca tttaacggta ttgaggatgc agaaaataat actttgttga
    11461 aactggatga aggaagcaat acggcctccg gtttgggtat agaaatattg gacgcaaata
    11521 tgcgtccggt gaaactgaat gaccttcatg ccgggatgca gtggatccca ctggtaccag
    11581 aacagaacaa tattttgcct tactccgctc gtctgaagtc aactcagaag tccgtcaatc
    11641 cgggactggt gagggcttcg gcaaccttta cccttgaatt tcaataatca gagtcagata
    11701 tgaaaagact tcacaagagg ttcctgttag ctacgttttg cgcgttattc acagcaactc
    11761 tccaggccgc cgatgtcact atcactgtta atggtcgggt agtcgctaaa ccctgcacta
    11821 ttcaaaccaa agaagctaac gttaatctcg gggatcttta tacgcgcaat ctgcaacaac
    11881 ctggttctgc atctggctgg cacaatatta ctttgtcatt aaccgattgt ccggttgaaa
    11941 caagtgcagt gacggcaatc gtgacaggtt caactgacaa tacgggttat tacaaaaatg
    12001 aaggtactgc cgaaaatatt cagatagagc tgagggatga ccaggatgct gcgttaaaaa
    12061 atggcgatag caaaacggtt attgttgatg agatcactcg taatgcacag tttccactta
    12121 aggcaagagc tatcacggtg aatggaaacg caagccaggg aacgatcgag gcgctaatca
    12181 atgtgatcta cacctggcaa taacgtcgat tcgattttct tagccttctt tttaaatcaa
    12241 tttttaacct taggatatat catgggtaaa acaatcagta taaaggtcct gttcggtatt
    12301 tatcttctat taatggcagg caaagttttt gccttcagtt gtaatgtcga tggcgggtca
    12361 agtattggag caggtacaac ctctgtttat gttaatctcg accctgtaat acagccgggc
    12421 cagaatctgg ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg
    12481 tacgacactg atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca
    12541 tataaaggat cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc
    12601 aacgtattgg atattggtga taagacaccg atgccattac cgttaaaact ctatattacc
    12661 ccagttgggg cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtatacat
    12721 atgtataaaa ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt
    12781 tcaaataaca atgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc
    12841 gtggatctcc cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc
    12901 gaacaaaagc tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt
    12961 gctaacacag cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat
    13021 ggaaaaatac tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta
    13081 cctctgggtt taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc
    13141 cagtcagtca ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg
    13201 cgatccattc acgaaaattg cgcggataat tattttgtga aggctattag cctacacctg
    13261 taagaaaatc cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta
    13321 atttaaacac tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata
    13381 atagattaaa aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat
    13441 ccctttgtat gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag
    13501 gataagtaga tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggggct
    13561 gttaaatccg tagcgaatgc agtacgtaag cagatggata tacgccagga tgttattgcc
    13621 atgtctgaca tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct
    13681 aagcacagtg cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc
    13741 acggataagc aggtaaatgc ctctttcttt gctgagaata cggttcaatc attactttcc
    13801 tggggagatc acgagcttga ggctgcggga cgtctcactc agcctttgaa atatgatgcc
    13861 gtcagcgact gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc
    13921 cttcaaagct atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat
    13981 gaagctgcct ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac
    14041 tgctccaaca tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt
    14101 aaagggaccg tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat
    14161 aaccctggta caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga
    14221 gcgaaaatga tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg
    14281 caaaacccgt ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac
    14341 gtgcgcattg gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc
    14401 gatgatgctg caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg
    14461 cataccgtcg gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc
    14521 gaacgtattt ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct
    14581 gccgaacgca ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag
    14641 aacgtacagc aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg
    14701 ggtatatgcc cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc
    14761 gagaaaccgt ctgcagagtt tctggctcgc ctgggtgagc gctatggctt caccccacct
    14821 catgcacctg gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga
    14881 gcattgatct gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct
    14941 gtaccgttaa cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat
    15001 ctgctgaccg cacggcatag ctatatcctg ccggttctgg ggcgtagcga gattgatatg
    15061 caaaaaagcg gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg
    15121 cgtggcgtgt taaaacccgc cggtgtaatg ctgaaatcag agtgtgctgt ggtcgcggga
    15181 atcgcccagg caacactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat
    15241 gatcgcattc gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc
    15301 atccgtcatc ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg
    15361 ccgtcaggta aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg
    15421 tttaacagta agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt
    15481 tatggtatgg atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt
    15541 gctaaacaag ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt
    15601 acgccagacg gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct
    15661 atggctgacc gctcactggt gacctatttt ccagaatcga atcacatgct aactcttgat
    15721 aaccacgatc cattaagtgg cattcctggt tataaaagta ttccggttga attagaacca
    15781 tcaaattaat tcgcctcctc atttcttctg ctgtcatccg cacagcagaa gaatttctca
    15841 ttgactatta tcgcgcaatt tgctcacatt gattaaatta aaccacacac tataagatat
    15901 aaacctctgc ctacagctgt aagaaactcc gctcagtact gaagtgccag tcctatttcc
    15961 tcttttctcc agcctgttat attaagcata ctgattaacg atttttaacg ttatccgcta
    16021 aataaacata tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagacaa
    16081 ctataaagaa atcatgtctg taattgtggt tgtcttatta ctgaccctta cgttgatagc
    16141 cattttttcg gcaattgatc agctgggtat ttcagaaatg ggtcgcattg caagagatct
    16201 tacacatttc attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatatga
    16261 aatgttaaaa aagtatcgat aaa
//
LOCUS       DAZGTR010000096        16220 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGTR010000096 DAZGTR010000000
VERSION     DAZGTR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.096"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            93..1382
                     /gene="hpaG"
                     /locus_tag="ACHLCY_003491"
     CDS             93..1382
                     /gene="hpaG"
                     /locus_tag="ACHLCY_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFP0370672.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKIREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMYYVTGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPIL
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            1379..2845
                     /gene="hpaE"
                     /locus_tag="ACHLCY_003492"
     CDS             1379..2845
                     /gene="hpaE"
                     /locus_tag="ACHLCY_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0370673.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2847..3698
                     /gene="hpaD"
                     /locus_tag="ACHLCY_003493"
     CDS             2847..3698
                     /gene="hpaD"
                     /locus_tag="ACHLCY_003493"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFP0370674.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3708..4088
                     /locus_tag="ACHLCY_003494"
     CDS             3708..4088
                     /locus_tag="ACHLCY_003494"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFP0370675.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4156..4959
                     /gene="hpaH"
                     /locus_tag="ACHLCY_003495"
     CDS             4156..4959
                     /gene="hpaH"
                     /locus_tag="ACHLCY_003495"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFP0370676.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4970..5758
                     /gene="hpaI"
                     /locus_tag="ACHLCY_003496"
     CDS             4970..5758
                     /gene="hpaI"
                     /locus_tag="ACHLCY_003496"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFP0370677.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5828..7204
                     /gene="hpaX"
                     /locus_tag="ACHLCY_003497"
     CDS             5828..7204
                     /gene="hpaX"
                     /locus_tag="ACHLCY_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFP0370678.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7214..8104
                     /gene="hpaA"
                     /locus_tag="ACHLCY_003498"
     CDS             7214..8104
                     /gene="hpaA"
                     /locus_tag="ACHLCY_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFP0370679.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8355..9917
                     /gene="hpaB"
                     /locus_tag="ACHLCY_003499"
     CDS             8355..9917
                     /gene="hpaB"
                     /locus_tag="ACHLCY_003499"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFP0370680.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQLDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9935..10447
                     /locus_tag="ACHLCY_003500"
     CDS             9935..10447
                     /locus_tag="ACHLCY_003500"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFP0370681.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10840..12990
                     /gene="btsT"
                     /locus_tag="ACHLCY_003501"
     CDS             10840..12990
                     /gene="btsT"
                     /locus_tag="ACHLCY_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP0370682.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13040..13243
                     /locus_tag="ACHLCY_003502"
     CDS             13040..13243
                     /locus_tag="ACHLCY_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0370683.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13254..14210
                     /gene="yjiA"
                     /locus_tag="ACHLCY_003503"
     CDS             13254..14210
                     /gene="yjiA"
                     /locus_tag="ACHLCY_003503"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0370684.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14288..16220)
                     /locus_tag="ACHLCY_003504"
                     /pseudo
     CDS             complement(14288..16220)
                     /locus_tag="ACHLCY_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
BASE COUNT         3761 a         4454 c         4343 g         3662 t
ORIGIN      
        1 tacatcactt acttttgcca aactacgcaa tcgggaataa aagtaatcat taacatatta
       61 atgattaaaa tgatcgaatc cgaggagtgg taatgaaagg cactatcttc gccgtagcgt
      121 tgaaccatcg cagccaactt gatgcatggc aggaagcgtt ccagcaatcc ccctacaaag
      181 ccccgcctaa aactgcggtc tggtttatta aaccgcgcaa tacggtgatt ggttgcggtg
      241 aaccgattcc ctttccacag ggtgaaaagg tactgagcgg tgcgactgtt gcgctgattg
      301 tgggaaaaac ggcgacgaaa atacgtgaag aagatgcagc agagtacatc gccggatatg
      361 cgctggctaa cgatgtcagc ctgccggaag agagctttta ccgcccggca atcaaagcaa
      421 aatgtcgtga tggattctgc cccattggcg aaaccgtggc tctcagcaat gtcgataatc
      481 tgaccatcta taccgagatc aacgggcgtc ctgccgatca ctggaatact gccgatttac
      541 aacgtaacgc cgcacagctg ctgagcgccc tgagcgaatt tgccacgctg aacccaggcg
      601 atgccattct gctcggcacg ccacaggcgc gcgtggaaat acagccaggc gatcgcgtgc
      661 gtgttctcgc agaaggtttc ccgccgctgg aaaatccggt agtggacgaa cgtgaagtga
      721 ccacgcgcaa gagcttccca acgctgccac acccgcacgg tacgctgttt gccctcggcc
      781 tgaactacgc cgaccacgcc agcgaactgg aatttaagcc accggaagaa ccgctggtgt
      841 tcctgaaagc gccgaatacc ctcactggcg ataaccagac ctccgtgcgc ccgaacaata
      901 ttgaatacat gcactacgaa gcggagctgg tggtagttat tggcaagcag gcgcgtaacg
      961 tcagcgaagc cgatgccatg tattatgtca cgggctacac cgtgtgtaac gactacgcca
     1021 ttcgcgacta tctggaaaac tactaccgcc ctaacctgcg ggtaaaaagc cgcgacggac
     1081 tgacgccgat actttcaacc atcgtgccga aagaggcgat cccggacccg cataatctga
     1141 cccttcgcac cttcgtcaac ggcgagttac gccagcaagg caccaccgcc gatctgatct
     1201 tcagcgtgcc cttcctgatc gcctacttaa gcgaatttat gaccctgaat ccgggcgaca
     1261 tgatcgccac cggcacacca aaaggcttat ctgacgtggt gcctggcgat gaagtagtgg
     1321 tggaagtaga aggcgtgggc cgcctggtga accgaattgt gagtgaggat acagcgaaat
     1381 gaaaaaagta aatcattgga tcaacggcaa aaatgtcgca ggtaacgact acttccagac
     1441 caccaatccg gcaacgggtg aagtgctggc ggatgtggcc tctggcggtg aagcggagat
     1501 caatcaggcg gtagcggcag cgaaagaggc gttcccgaaa tgggccaatc tgccgatgaa
     1561 agagcgtgcg cgcctgatgc gccgtctggg cgatctgatc gaccagaacg tgcctgagat
     1621 cgccgcgatg gaaaccgccg acacgggcct gccaatccat cagaccaaaa atgtgttgat
     1681 cccacgcgct tcccacaact ttgaattttt cgcggaagtc tgccagcaga tgaacggcaa
     1741 gacttatccg gtcgacgaca agatgctcaa ctacacgctg gtgcagccgg taggcgtttg
     1801 tgcactggtg tcaccgtgga acgtgccgtt tatgaccgcc acctggaagg tcgcgccgtg
     1861 tctggcgctg ggcaataccg cggtactgaa aatgtcggaa ctctccccgc tgaccgccga
     1921 ccgcctgggt gagctggcgc tggaagctgg tattccggcg ggcgtgctga acgtggtaca
     1981 gggctacggc gcaaccgcag gcgatgcgct ggtccgtcat catgacgtgc gtgccgtgtc
     2041 gttcaccggc ggtacggcga ccgggcgcaa catcatgaaa aacgccgggc tgaaaaaata
     2101 ctccatggaa ctgggcggta aatcgccggt gctgattttt gaagatgctg atattgaacg
     2161 cgcgctggac gccgccctgt tcaccatctt ctcgatcaac ggcgaacgct gcaccgccgg
     2221 ttcgcgcatc tttattcagc aaagcatcta cccggaattc gttaaacgct ttgccgaacg
     2281 cgccaaccgt ctgcgcgtgg gcgatccgaa cgatccgaat acccaggttg gcgcgcttat
     2341 cagccagcag cactgggaaa aagtctccgg ctatatccgt ctcggcattg aagaaggcgc
     2401 aaccctgctg gcgggcggcc cggataaacc gtccgacctg cctgcgcacc tgaaaggcgg
     2461 caacttcctg cgcccaaccg tgctggcaga cgttgataac cgtatgcgcg tcgcccagga
     2521 agagattttc gggccggtcg cctgcctgct gccgtttaaa gacgaagctg aaggcttacg
     2581 cctggcaaac gacgtggagt acggcctcgc gtcgtacatc tggactcagg atgtcagcaa
     2641 agtgctgcgt ctggcgcgcg ggattgaagc gggcatggtg ttcgtcaaca cccagaacgt
     2701 gcgtgatttg cgccaaccgt ttggcggcgt aaaagcctcc ggcaccgggc gtgaaggcgg
     2761 tgagtacagc ttcgaagtgt tcgcggaaat gaagaacgtc tgcatttcca tgggcgacca
     2821 tccaattccg aaatggggag tctgatatgg gtaagttagc gttagccgcc aagatcacgc
     2881 acgtaccgtc gatgtatctc tctgaactgc cagggaaaaa ccacggttgc cgccagggcg
     2941 cgatcgacgg gcataaagag atcagcaagc gttgccggga aatgggcgtc gataccatta
     3001 tcgttttcga tacccactgg ctggtcaaca gtgcttatca catcaactgt gcagaccatt
     3061 ttgaaggcat ctacaccagc aacgagctgc cgcattttat ccgcgatatg acctacaact
     3121 acgagggcaa cccggagttg gggcagctta ttgccgatga agccttaaag ctcggcgtgc
     3181 gggcaaaagc gcacaacatt cccagcctga aactggaata cggcacgctg gtaccgatgc
     3241 gctacatgaa tgaagacaag cacttcaaag tggtctccat ttcggctttc tgcacggttc
     3301 acgattttgc tgacagccgc aagctgggcg aagcgattct gaaagcgatc gaacagtacg
     3361 acggcaccgt ggcggtcctt gccagcggtt cgttatcgca ccgctttatt gacgatcagc
     3421 gtgcagaaga agggatgaac agctacaccc gcgagttcga ccgccagatg gacgagcgcg
     3481 tggtgaagtt gtggcgcgaa ggccagttca aagagttctg caatatgctg ccggagtacg
     3541 ccgactactg ctacggcgaa ggcaatatgc acgacacggt gatgctgctg gggatgctcg
     3601 gctgggataa atacgacggc aaggtggagt ttatcaccga gctattccca agctctggca
     3661 ccggtcaggt taacgctgtt ttcccgcttc ccgcgtaagg aaccaccatg ccgcacttta
     3721 tcgtggaatg tactgaaaat attcgtgaag aagcccgctt acctgagctg tttgccagtg
     3781 ttaacactgc actggcggca acggggattt ttccgcttgg gggaatacgt agccgggcgc
     3841 actggatcga tacctggcaa atggctgatg gcaagcacga ttacgccttt gtgcatatga
     3901 cgctaaaaat cggcagcggg cgcagcctgg aaagccgtca ggaagtgggc gaaatgctgt
     3961 ttgatctgat taaaacgcac ttcgcctcac tgatggagag ccgttacctg gcgctttcat
     4021 ttgagattgc agagctgcac ccgacgctga attttaaaca aaacaacgtg cacgcgttgt
     4081 ttaaataaat ttgcaccagc ttccaggccg gatgataagg cgcagccgca tccggcaatg
     4141 ccacaggata tcgctatgtt cgataaacac acccacaccc tgatcgccca gcgtctggat
     4201 caggcagaaa aacagcgcga acagatccgc gcgatctcgc tggattaccc ggagatcacc
     4261 atcgaagacg cttacgcggt gcagcgtgaa tgggttcgac tgaaaatcgc cgaaggtcgc
     4321 acgctgaaag gccacaaaat cggcctgacc tcgaaagcga tgcaggccag ctcacaaatc
     4381 agcgagccgg attacggtgc cctgctggat gacatgttct tccacgatgg cagcgatatc
     4441 ccgaccgatc gctttatcgt gccgcgcatt gaagtggagc tggctttcgt gctggcaaaa
     4501 ccgctgcgtg ggccaaactg cacgctgttc gacgtctaca acgccacgga ctatgtgatc
     4561 ccggcgctgg agctgatcga cgctcgctgc cacaacatcg atccggaaac ccagcgcccg
     4621 cgtaaagtgt tcgacaccat ttctgataac gccgccaatg ccggggtgat cctcggtggt
     4681 cgtcccatta agcccgatga gttggatcta cgttggatct ccgccctgat gtatcgcaat
     4741 ggcgtgattg aagaaaccgg cgtcgccgct ggtgtgctga atcatccggc aaacggcgtg
     4801 gcctggctgg cgaacaaact cgcaccgtat gacgtacaac tggaagccgg gcaaatcatt
     4861 ctcggcggtt cgttcacccg cccggttccg gcgcgtaagg gcgacacctt ccacgtcgat
     4921 tacggcaaca tgggctccat tagctgccgc tttgtttaag gagagaacga tggaaaacag
     4981 ttttaaagcg gcgctgaaag caggccgccc gcagatcgga ttatggctgg ggctgagtag
     5041 cagctacagc gcagagttac tggccggagc aggattcgac tggttattga tcgacggtga
     5101 gcacgcgccg aataacgtgc aaaccgtgct cacccagcta caggcgattg cgccctaccc
     5161 cagccagccg gtggtacgtc cgtcgtggaa cgatccggtg caaatcaaac aactgctgga
     5221 cgtcggcaca caaaccttgc tggtgccgat ggtacaaaac gctgacgaag cccgtgaagc
     5281 ggtacgcgcc acccgttatc cccccgccgg tattcgcggt gtgggcagtg cgctggcccg
     5341 cgcctcgcgc tggaatcgta ttcctgatta cctgcaaaaa gccaacgatc aaatgtgcgt
     5401 gctggtgcag atcgaaacgc gtgaggcaat gaagaactta ccgcagattc tggacgtgga
     5461 aggcgtcgac ggcgtgttta tcggcccggc ggatctgagc gccgatatgg gttatgccgg
     5521 taatccgcag cacccggaag tacaggccgc cattgagcag gcgatcgtgc agatccgtga
     5581 atcgggcaaa gcgccgggga tcctgatcgc caatgagcaa ctggcaaaac gctatctgga
     5641 actgggcgcg ctgtttgtcg ccgtcggcgt tgacaccacc ctgctcgccc gcgccgctga
     5701 agcgctggca gcacggtttg gtgcgcaggc caccgccgtg aagcccggcg tgtattaatg
     5761 ccggatgcgc tgcgcatccg ccataaacct tgtcgtaccc tacaaaaatc ccattagagg
     5821 aagaaaaatg agcgacacct cacctgccat accggagagt atcgatccgg cgaatcagca
     5881 taaagcgctg actgccggac aacaggcggt tattaagaag ctatttcgcc gcctgatcgt
     5941 ctttctgttc gtgctgttta tcttctcgtt ccttgatcgc atcaacatcg gctttgccgg
     6001 actcacgatg ggacgcgacc tcggtctgag cgctaccatg tttggcctcg ctaccaccct
     6061 gttctatgcc gcttatgtca tcttcggcat tcccagcaac attatgctga gtattgtcgg
     6121 tgcgcggcgc tggatcgcca ccatcatggt gctctggggc atcgcctcta ctgccaccat
     6181 gtttgccact ggccccacca gcttgtacgt actgcgtata ctggttggca ttaccgaagc
     6241 cggctttctg cctggtattc tgctgtattt aaccttctgg tttccggcct atttccgcgc
     6301 ccgtgccaac gccttgttta tggtagcaat gccggtaacg acagcgttgg gctcgatcgt
     6361 ttccggctac attttgtcgc tggatggcgt catggcatta aaaggctggc agtggctgtt
     6421 tttgctggaa ggcttcccgt cggtattact cggcgtcatg gtgtggttct ggcttgatga
     6481 ctcaccggac aaagctaagt ggctgacgaa agaagacaaa aaatgcctgc aagagatgat
     6541 ggataacgat cgtctgacgc tggttcagcc agagggagcc atcagccacc acgccatgca
     6601 acaacgcagt atgtggcggg agatcttcac tccggtggtg atgatgtata ccctggcgta
     6661 tttctgcctg accaacacac ttagtgcgat cagcatctgg acaccgcaga tcctgcaaag
     6721 ttttaatcag ggcagcagta atatcaccat cggcctgctg gccgccgtac cgcagatttg
     6781 taccattctc gggatggtct actggagccg tcactcagat cgccgccagg aacgaaggca
     6841 tcacaccgcc cttccttatt tgttcgctgc cgcaggttgg ttactggctt cggcaactga
     6901 tcacaacatg atccagatgc tggggatcat tatggcttcg accggatcat tcagcgcaat
     6961 ggcgattttc tggacaacac cggatcagtc catcagcctg cgggcacgag cgatcggtat
     7021 tgcggtgatc aacgccactg gcaacattgg ttcagcgtta agtccgttta tgatcggctg
     7081 gttgaaagat ttgaccggca gctttaacag tggattgtgg tttgttgccg cgctgctggt
     7141 gattggtgcg gggattatct gggcgattcc aatgcagtcc tcccgtccgc gagcgacccc
     7201 gtaaggaacg acgatgtgtg accgtcagat tgccaatatt gatatcagca aagagtacga
     7261 tgaaagcctg ggcacggacg atgtgcatta tcagtcgttc gcccgcatgg cggccttttt
     7321 tggccgccat atgctgccac atcgccacga acagtacttt cagatgcatt tcctcaatag
     7381 cggacagatt gagctacaac ttgacgatca tcgctactcg gtggaagcgc ccctgtttgt
     7441 cctgacgccg ccgtcagtac ctcatgcgtt tattacggag tctgacgccg acggtcatgt
     7501 gttgacggta cgggaagatc tgatctggcc cctgctggaa gttctttatc cgggcactcg
     7561 ggaaaccttc ggcctgccgg ggatttgcct gtcactggca gataaacccg acgaactggc
     7621 ggcgctggaa cactattggc aactgataga gcgggaatcg gtagaacaac tgcctggacg
     7681 ggaacacacc ctgacgttac tggcacaggc agtattcacc ctactgctgc gtaatgcaaa
     7741 actcgacgac catgccgcca gcggaatgcg cggagaatta aaactgttcc agcgttttaa
     7801 tatgctgatt gaaagccatt ttcatcagca ctggacagta ccggattacg ctaacgaact
     7861 gcatatcacc gaatcacgcc tcacggacat ctgccgccgc tttgccaacc gtccaccaaa
     7921 acggttgatt ttcgacaggc agctgcgaga agccaaacgg ctgctgctgt tttctgataa
     7981 cgcagtgaac aatattgcct ggcaactcgg ttttaaagat ccggcttatt ttgcccgctt
     8041 ttttaatcgc ttagtcggtt gctcgcccag tgcttatcgc gccaaaaaag tacctgtgac
     8101 gtgaaaatcc cttagcctta acgggaaacc aggcaccacc tgctattccc ctttctccgc
     8161 ctggagcaag gggatttagt aacgtcaaaa atcgctaaaa gcgacctcga tcacaaaacc
     8221 gtacccgacc tgaaaagtac cagccattgt cccaaaagtc tcttcccgta atcgcccctg
     8281 caagcttcaa ttgaataaca aaaacaacac acaaaattaa caatcgtatt ccgattaata
     8341 ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt ccgttcaccg
     8401 gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag
     8461 tgaaagacgt cactactcat ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt
     8521 acgacgcgct acacaaaccg gagatgcagg actctctgtg ctggaacacc gacaccggca
     8581 gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc
     8641 aacgcgatgc catcgctgag tggtcacgcc tgagctatgg ctggatgggc cgtaccccag
     8701 actacaaagc tgctttcggt tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg
     8761 agcagaacgc ccgtaactgg tacacccgta ttcaggaaac tggcctctac tttaaccacg
     8821 cgattgttaa cccaccgatc gatcgtcatt tgccgaccga taaagtaaaa gacgtttaca
     8881 tcaagctgga aaaagagact gacgccggga ttatcgtcag cggtgcgaaa gtggttgcca
     8941 ccaactcggc gctgactcac tacaacatga ttggcttcgg ctcggcacaa gtgatgggcg
     9001 aaaacccgga cttcgcactg atgttcgttg cgccaatgga tgccgatggc gtgaaattaa
     9061 tctcccgcgc ctcttatgag atggtcgcgg gtgctaccgg ctcgccatac gactacccgc
     9121 tctccagccg cttcgatgag aacgatgcga ttctggtgat ggataacgtg ctgatcccat
     9181 gggaaaacgt actgatctac cgcgatttcg atcgctgccg tcgctggaca atggaaggcg
     9241 gttttgcccg tatgtatccg ctgcaagcct gtgtgcgcct ggcagtgaaa ctcgacttca
     9301 ttacagcact gctgaaaaaa tcactcgaat gtaccggcac cctggagttc cgtggtgtac
     9361 aggccgatct cggtgaagtg gtggcgtggc gcaacacctt ctgggcattg agtgactcga
     9421 tgtgttctga agcgacgccg tgggtcaacg gggcttattt accggatcat gccgcactgc
     9481 aaacctatcg cgtactggca ccaatggcct acgcgaagat caaaaacatt atcgaacgca
     9541 acgttaccag tggcctgatc tatctccctt ccagtgcccg tgacctgaac aatccgcagc
     9601 tcgaccagta tctggcgaag tatgtgcgcg gttcgaatgg tatggatcac gtccagcgca
     9661 tcaagatcct caaactgatg tgggacgcca ttggcagcga gtttggtggt cgccacgaac
     9721 tgtatgaaat caactactcc ggtagccagg atgagattcg cctacagtgt ctgcgccagg
     9781 cacaaagctc cggcaatatg gacaagatga tggcgatggt tgatcgctgc ctgtcggaat
     9841 acgaccagaa cggctggact gtgccgcact tgcacaacaa cgacgatatc aacatgctgg
     9901 ataagctgct gaaataacgc agcaggaggt taagatgcaa ttagatgaac agcgcctgcg
     9961 ctttcgtgac gcgatggcca gcctgtcggc agcggtaaat attatcacca ccgagggcga
    10021 cgccggacaa tgcgggatta cggcaacggc cgtctgctcg gtcacggata caccaccatc
    10081 gctgatggtg tgcattaacg ccaacagtgc gatgaacccg gtttttcagg gcaacggtaa
    10141 gttgtgcgtc aacgtcctca accatgagca ggaactgatg gcacgccact tcgcgggcat
    10201 gacaggcatg gcgatggaag agcgttttag cctctcatgc tggcaaaaag gtccgctggc
    10261 gcagccggtg ctgaaaggtt cgctggccag tcttgaaggt gagatccgcg atgtgcaggc
    10321 aattggcaca catctggtgt atctggtgga gattaaaaac atcatcctca gtgcagaagg
    10381 tcacggactt atctacttta aacgccgttt ccatccggtg atgctggaaa tggaagctgc
    10441 gatttaagct ccagcccctt cacgcattcg tcgaaggggc atttttattg tgatctcaat
    10501 cacatatcca cccgatgaat aattcttgcc caaccgctca ccgccattcc tgaccgctta
    10561 cgtccctaaa ataaccactc agttatttac cttactttac gcgcgcgtaa ctctggcaac
    10621 atcactgcag gatagcggtc aatttacctc ctcaaacgca acgcaaacct agaacggctt
    10681 cggcctatga ttaacaaatt actctatgaa ttttggtttg caggaaggca gcaagtgagt
    10741 gaatccccgg gagcttacaa cagtaagtga caggggtgaa cgaacgcaac tgccgcacct
    10801 gtaagccaaa agacgacgag taaaatgcca ggttttacta tggatacgaa aaaactattc
    10861 aagcacatac cctgggtgat tctcggaatc atcggtgcat tctgcctcgc ggtagttgca
    10921 ttacgtcggg gggagcacgt cagcgccctg tggatcgtgg tcgcctctgt gtcggtgtat
    10981 ctggtggcgt atcgctacta cagtctgtac atcgcccaga aggtgatgaa actcgacccc
    11041 acgcgcgcga cgcctgcggt tattaacaac gacggtctga actacgttcc gaccaaccgt
    11101 tacgtgttgt ttggtcacca cttcgccgct atcgccggtg ctggtccgct ggtcggtccg
    11161 gttctcgccg cacagatggg ctacctgcct ggcacgctgt ggctgctggc gggggtagtg
    11221 ctggccggtg cggtacagga ctttatggtg ctgtttatct cctctcgccg taacggcgca
    11281 tctctcggtg agatgatcaa agaagagatg gggccagtac cggggactat cgcgctgttt
    11341 ggctgtttct taatcatgat catcatcctt gccgtcctgg cgctgattgt ggttaaagcc
    11401 ctggccgaaa gtccgtgggg tgtcttcacc gtttgctcaa ccgtaccgat tgcgctgttt
    11461 atgggtatct acatgcgctt tatccgtccg gggcgtgtgg gtgaagtctc tgtcattggt
    11521 atcgtgctgc tggttgcctc tatctacttc ggtggcgtga ttgctcacga tccgtactgg
    11581 ggtccggcac tgacctttaa agacaccacc attaccttcg cgctgattgg ctatgcgttt
    11641 gtttccgcat tgctgccggt gtggctgatc ctcgcaccgc gcgactatct ggcaaccttc
    11701 ctgaaaatcg gcgttatcgt cggcctggcg ctgggtatcg tggtgctgaa cccggaactg
    11761 aaaatgcctg cgatgaccca gtacattgac ggtactggcc cgctgtggaa aggtgctctg
    11821 ttcccgttct tgttcattac catcgcctgt ggtgcggtat ctggcttcca cgcactgatc
    11881 tcttccggta cgacgccgaa actgctggct aatgaaaccg acgcgcgttt cattggctac
    11941 ggcgcaatgc tgatggagtc cttcgtggcg attatggcgc tggttgctgc gtccatcatc
    12001 gaaccgggtc tttacttcgc gatgaacacc ccgcctgctg gccttggcat caccatgcct
    12061 aacctgcatg aaatgggtgg cgagaacgcg ccgatcatca tggcgcagct gaaagacgtt
    12121 accgcacacg cggcagcgac cgtcagctcc tggggcttcg tgatttcgcc agagcagatc
    12181 ctgcaaaccg cgaaagacat tggtgagcct tctgtcctga accgtgcagg tggtgcgcca
    12241 acgctggcgg taggtatcgc tcacgtgttc cacaaagtgc tgccgatggc tgacatgggc
    12301 ttctggtatc acttcggtat tctgttcgaa gccctgttca tcctgaccgc gctggatgcg
    12361 ggtactcgtt ctggccgctt tatgctgcaa gacctgctgg gtaacttcat cccgttcctg
    12421 aaaaaaaccg actctctggt tgccggtatc atcggtactg cgggctgtgt gggtctgtgg
    12481 ggctacctgc tgtatcaggg cgtggtcgat ccgctgggcg gcgttaagag cctgtggccg
    12541 ctgttcggta tttccaacca gatgctggca gccgtagcgc tggtactggg caccgttgtg
    12601 ctgattaaga tgaagcgcac ccaatacatc tgggtaactg ttgttccggc tgtatggctg
    12661 cttatctgca ccacctgggc gctgggcctg aaactgttca gcaccaaccc gcagatggaa
    12721 ggcttcttct acatggcaag ccagtacaaa gagaagattg ctaacggtac tgacctgacg
    12781 gcgcagcaga ttgccaacat gaaccacatc gttgtaaaca actacaccaa cgcaggcctg
    12841 agtattctgt tcctgattgt ggtgtacagc atcatcttct acggtttcaa aacctggctt
    12901 gcggtgcgta acagcgacaa acgtactgac aaagaaactc cgtacgttcc aatcccggaa
    12961 ggcggcgtga agatctcttc gcaccactaa ccgtgtttag ccccgcttcg gcggggcttt
    13021 gttcttatca gagtgaacta tgtttggtaa cttaggacag gcaaaaaaat atctcggcca
    13081 ggcggcgaag atgttgattg gcattccaga ctatgacaac tacgtcgagc atatgaagac
    13141 caaccatccc gacaagccgt acatgagcta tgaagaattc ttccgcgaac gccagaacgc
    13201 acgctacggc ggcgatggta aaggcggtat gcgctgttgt taatggagag agcatgaacc
    13261 cgattgcagt taccctactc accggttttt taggcgcggg caaaaccacc ctgctgcgcc
    13321 atattcttaa tgaacaacac ggctacaaga ttgccgtgat tgaaaacgaa ttcggcgaag
    13381 tctccgttga tgatcaattg attggtgatc gcgcaacgca gatcaaaacg ctgaccaacg
    13441 gctgcatctg ctgttcgcgc tccaacgagc tggaggacgc gctactcgac ctgctggata
    13501 atctcgacaa gggcaatatt cagttcgatc gtctggtcat tgaatgcacc ggcatggccg
    13561 atcctggccc gattattcaa acctttttct cccatgagat tttatgccag cgctacctgc
    13621 tggacggcgt gattgcgctg gtggatgcgg tacatgccga tgagcagatg aaccagttca
    13681 ccatcgccca gtcccaggtg ggctacgccg accgtattct gctgactaaa accgacgtcg
    13741 caggcgaagc agaaaaactg cgtgaacgtc tggcacgcat caacgcccgt gcaccggtct
    13801 acaccgtcac ccacggcgac atcgacctgg gtctgctgtt caacaccaac ggttttatgc
    13861 tggaagaaaa cgtcgtcagc accaaaccgc gtttccattt tatcgcggac aaacaaaacg
    13921 atatttcgtc gattgtggtg gaactggatt acccggtaga tatcagcgaa gtttcccggg
    13981 tgatggaaaa cctgctgctg gaatcggcgg ataaactgct gcgttacaaa gggatgctgt
    14041 ggattgacgg cgaacctaac cgcctgctgt tccagggcgt ccagcgcctc tacagcgccg
    14101 actgggacag gccatggggc gatgaaaaac cgcatagcac gatggtgttt atcgggattc
    14161 agttgccgga ggatgagatt cgggcggcgt ttgcgggact caaaaagtaa ccagccctcc
    14221 aacatcacaa tacctgagac tttatcaccc tcatattcct ccagaatatg agggcattcg
    14281 ggcttaatta tctctgaacg tgcaacactt cctgcgactc cttatgcgcc tccacatcct
    14341 gcttcgttaa ccagagcgac ttacgaccaa aattttcgta cattttcagc tgatcttcat
    14401 agtgcttatc aactgttcca tcgggagcaa taaacccact ctgaccgggt gcgaccacat
    14461 cccaggcaag cacaggacga tcgcttgtcg ttggtgagaa aacaatcata tcgttttctg
    14521 ttccacggtt ttgatactcc gcctgatgac gcgtttcttc cgctgcggcc tgcggtacac
    14581 caaagaaatt atttgcccgg aacgttaagg ccattgcagg tgttttccag ttactcacat
    14641 tattgccata gcgtttggaa agagtctccc aggtatcttc cagcgcagcc aacacaacct
    14701 cctgctgtgg tttcccagca aacagatcaa ccgcctgtgg gattggtgat ttgtctccct
    14761 gcaccgcctc atacaaaatt tttgctccaa cacttatatt cagcgaacca gttgggccgt
    14821 cctgggttgt ttcgtagcca ctggcgctgt accacttatc aaatggcata ggtacggcag
    14881 ccactacggt acgcttcaac atactggtca gccaaacgtt caggatggca gagcctggct
    14941 gctgccaggt tttaccatca tcattaagca aattgatgcc atcccaacgt gttaatgtat
    15001 ccaccaactg acggcgcgga tcgctctgtg tcaaaccaga tgtcgctgct tgcagagtag
    15061 gtaaaaaaag cctcaggtta agatcctgac gactggtttg gcgaataaca tcccatgcct
    15121 gatcagcagt taagcgtggc ttttgctcaa gcagtcggtc gatctccgta acgcgatctg
    15181 cccccccccc acaaaaaggc aaacagatcc gaagcgggat aatctttttg gggagaattg
    15241 ttccagttag caatatatcc cgacaggggg ttatacacct tagggttcat ttcaaaaggc
    15301 aatagccctt tccagtccca ttttcccgta ccaggaacgg gtaatcgcgg atcatggcct
    15361 gattgacgat ctggataagc accagtatga acataaccaa tattgccgtt tacatcagca
    15421 tagtaccagt tgatggtcag tgcttgtttc gctgcctgct gtgtccactc ctgccaattt
    15481 ttggccttca tctgatgagt ccaggccagc aaagacgcca cctctttacc atcccatgcg
    15541 cgggatttag cgtaagccgt ttgtgtcgtc tggtcagttt ggagaatgtt gccatgcacc
    15601 gtacgccaga cagtaaaggt ctctgcctga ccatttttca ccgtaatggt ttcctcacgg
    15661 cttaacattt tcacccactt accattatgc aagtagtagc ctggtttctc tgccgacagc
    15721 cgttcagcaa aaatatcgac atcatcgccg aaacctgccg ttgatcccca ggaaatcaca
    15781 ccattatgac caaaaaccag cccaggatag gcaaatggtg tattgccagt gacatcataa
    15841 ccagcaccgt gcagaccaat accataagta tacgcaggcg cataccagcc aaactgcgga
    15901 ccatttacca tgattgcttt cgcatcctgg gctttgcttt tgccgatcac ccacatattg
    15961 ctggtcgttg gataccccgc cagaccattg gcaccaccct gtgcaaattg tgcagcaata
    16021 gtttcccggt tcttccctgc tgttaacgcc agcagtgcgc catccgcccc ttttgctggt
    16081 cggtcaagca ttggtgcagg taaatcgtag cgtggcaaca gagctgctgt ttgcgagttt
    16141 tgctgattaa atttaagtgg gtagctactc tcttgtacgg caatagtggt tggcgctgat
    16201 gggtttacca gccatttcaa
//
LOCUS       DAZGTR010000097        16208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGTR010000097 DAZGTR010000000
VERSION     DAZGTR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.097"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..608)
                     /locus_tag="ACHLCY_003505"
     CDS             complement(<1..608)
                     /locus_tag="ACHLCY_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFP0370685.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIDALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFN"
     gene            complement(960..1124)
                     /gene="yjiS"
                     /locus_tag="ACHLCY_003506"
     CDS             complement(960..1124)
                     /gene="yjiS"
                     /locus_tag="ACHLCY_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP0370686.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAVRRWRRQMQARRVLQQMSDERLR
                     DIGLRREDVE"
     gene            1301..2713
                     /gene="yjiR"
                     /locus_tag="ACHLCY_003507"
     CDS             1301..2713
                     /gene="yjiR"
                     /locus_tag="ACHLCY_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0370687.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGISISTVQ
                     QAYQTLETMKFITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGDAVYRAME"
     gene            complement(2958..3914)
                     /locus_tag="ACHLCY_003508"
     CDS             complement(2958..3914)
                     /locus_tag="ACHLCY_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0370688.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKREPWIEY"
     gene            4381..5613
                     /gene="mdtM"
                     /locus_tag="ACHLCY_003509"
     CDS             4381..5613
                     /gene="mdtM"
                     /locus_tag="ACHLCY_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP0370689.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            5654..6934
                     /gene="yjiN"
                     /locus_tag="ACHLCY_003510"
     CDS             5654..6934
                     /gene="yjiN"
                     /locus_tag="ACHLCY_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP0370690.1"
                     /translation="MNKLVELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSLQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            7050..8201
                     /gene="yjiM"
                     /locus_tag="ACHLCY_003511"
     CDS             7050..8201
                     /gene="yjiM"
                     /locus_tag="ACHLCY_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP0370691.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            8211..8978
                     /gene="yjiL"
                     /locus_tag="ACHLCY_003512"
     CDS             8211..8978
                     /gene="yjiL"
                     /locus_tag="ACHLCY_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP0370692.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGTRFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            8975..9232
                     /gene="ytiA"
                     /locus_tag="ACHLCY_003513"
     CDS             8975..9232
                     /gene="ytiA"
                     /locus_tag="ACHLCY_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP0370693.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            9297..10157
                     /gene="yjiK"
                     /locus_tag="ACHLCY_003514"
     CDS             9297..10157
                     /gene="yjiK"
                     /locus_tag="ACHLCY_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0370694.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            10225..11403
                     /gene="yjiJ"
                     /locus_tag="ACHLCY_003515"
     CDS             10225..11403
                     /gene="yjiJ"
                     /locus_tag="ACHLCY_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0370695.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(11416..11970)
                     /gene="kptA"
                     /locus_tag="ACHLCY_003516"
     CDS             complement(11416..11970)
                     /gene="kptA"
                     /locus_tag="ACHLCY_003516"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP0370696.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            12220..12903
                     /gene="yjiH"
                     /locus_tag="ACHLCY_003517"
     CDS             12220..12903
                     /gene="yjiH"
                     /locus_tag="ACHLCY_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP0370697.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            12900..13361
                     /gene="yjiG"
                     /locus_tag="ACHLCY_003518"
     CDS             12900..13361
                     /gene="yjiG"
                     /locus_tag="ACHLCY_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP0370698.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTVHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            13374..14546
                     /gene="iadA"
                     /locus_tag="ACHLCY_003519"
     CDS             13374..14546
                     /gene="iadA"
                     /locus_tag="ACHLCY_003519"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0370699.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            14611..15522
                     /gene="hypT"
                     /locus_tag="ACHLCY_003520"
                     /gene_synonym="qseD"
     CDS             14611..15522
                     /gene="hypT"
                     /locus_tag="ACHLCY_003520"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP0370700.1"
                     /translation="MDDCGAILHNIETKWLYDFLPLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSNYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSRKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(15515..15907)
                     /gene="iraD"
                     /locus_tag="ACHLCY_003521"
     CDS             complement(15515..15907)
                     /gene="iraD"
                     /locus_tag="ACHLCY_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP0370701.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPLSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(15904..15987)
                     /gene="idlP"
                     /locus_tag="ACHLCY_003522"
     CDS             complement(15904..15987)
                     /gene="idlP"
                     /locus_tag="ACHLCY_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP0370702.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(16103..>16208)
                     /locus_tag="ACHLCY_003523"
     CDS             complement(16103..>16208)
                     /locus_tag="ACHLCY_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370703.1"
                     /translation="LSSGGDKYFHLHNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         3698 a         4113 c         4122 g         4275 t
ORIGIN      
        1 tgattaaata ccgccatgcc ttgtgataca ccatatttat cttttaaagc cgttagcagt
       61 gccagattat caatttcgct agtgctatca gagaagcggt ttgccatggt gcccacaaat
      121 atcatcgcga catcaaacgg ttcccagcgc ttaggagtaa agccaaatgt attaaactgt
      181 tttggtaaga gcgtctctgg attggtattt accttatcaa tccaggcatt cattccatca
      241 gcgtagcctt gcagaatgga catatcctct ggggaaaggg catcaatttg cgcccggata
      301 gcatccggcc agtagttacg acggatatct ttatcaaatt tcacaaaatc tttgccaagc
      361 acttccgcga cagtcccttg agtactgcga cgtgccattt ccatctgaaa aaggcgatct
      421 tgtgctacta catagccata gccataaaat aggtgccatg tatcattggc ataaatatgc
      481 ggcatgccgt attcatcgcg aacaatcttt atctcacttg acgactgctc agccagtgca
      541 ggtaagctcc aataatacat cagggaagca gtaacacagt tcacgatcat acgatttcta
      601 tttttcattg tatcctctgg caggtgtata attagcgaat tgatactagc aacgaagctt
      661 cggttatcta tttgcagaga attgtttcat tatgaactgt gagcgcgata actaattttt
      721 gatacaaaaa tttcattaat gtgatctaaa acgaatcgct ttattcaccg ctgatgattt
      781 tgttctattc ctttttgtga cagatccccc ggacgcatag atgatgaccc ccagagagac
      841 agctcaggtc attatctgta tgtaaatcag aaaaatatgt tgtaaacgac ttttatggtt
      901 ggattttgta gcaattttga atgcagacag gcagcatttg ccaacacgcc gcccatagtt
      961 cactccacat cctccctgcg taacccgata tccctcaacc gttcatcact catctgctgt
     1021 aacacccgtc gggcctgcat ttgccgccgc caacgcctca ccgcctgcca gagttgcaca
     1081 agtctgataa acggcgtttt ggctttgttt tcgtgaaatt ccatctgctg cctcctcatc
     1141 tttcagaggc ttcatcatcc gccttctggc agttcacaat acagatgcaa atattagttt
     1201 tatttaacat acagacaggt taaaacagca tctgcatccg gttttttagc ctcatctgta
     1261 ccggtttttc tttctgtatg ggccaaataa ggaatacagc atgacgcgtt atcaacatct
     1321 ggcgactcta cttgccgagc ggattgagca agggctgtat cgtcacgggg agaaattgcc
     1381 gtcggtgcgc agcttaagtc aggagcacgg catcagcatc agcaccgtgc agcaggcgta
     1441 ccagacgctg gagacgatga agttcatcac tccgcagccg cgttcgggtt attttgtcgc
     1501 acaacgtaaa gcccagccgc ctgtaccgcc gatgacgcgt ccggtgcagc gcccggtgga
     1561 aattacccag tgggatcagg tgctggatat gctggaggcg catagcgaca attccattgt
     1621 tccgttaagc aaaagcacgc cggatgtcga aacgcccagc ctgaaaccac tctggcgtga
     1681 gctaagccgg gtggtgcagc ataatctaca aaccgtgctc ggttatgact tgttagccgg
     1741 tcagcgagtg ttgcgggagc agattgcccg cctgatgctc gacagcggct cggtggtcac
     1801 cgccgatgac atcatcatca ccagcggctg ccataactcg atgtcgctgg cgttaatggc
     1861 ggtgtgtaaa ccgggcgata ttgtcgcggt cgaatctccc tgttattacg gttcgatgca
     1921 gatgctgcga ggcatgggcg tgaaagtgat tgaaatccca accgatccag aaactggcat
     1981 cagcgttgaa gcactggaac tggcgctgga acagtggccg attaaaggca tcattctggt
     2041 gccaaactgt aataatccgc tgggatttat tatgccggac gcacgcaaac gggccgttct
     2101 ctctctcgct cagcgtcatg atattgtgat ttttgaagat gatgtctacg gcgaactggc
     2161 aacggagtat ccgcgcccgc ggaccatcca ttcatgggat atcgacgggc gagtgctgtt
     2221 gtgcagctcg ttcagtaaaa gtattgctcc aggcctgcgc gtgggttggg tcgcaccggg
     2281 gcgttatcac gataaactga tgcatatgaa atacgccatc agcagcttta atgtgccgtc
     2341 cacgcaaatg gcggcggcaa cgtttgtgtt ggaaggccac tatcatcgcc atatccggcg
     2401 gatgcggcag acttatcagc gcaatctggc gctttatacc tgctggatac gggaatattt
     2461 tccctgcgaa atctgtatta cgcgcccgaa aggcggattt ttactgtgga tcgaattgcc
     2521 tgaacaggtc gatatggtct gcgtcgcgcg gcagctgtac cgcatgaaaa tccaggtggc
     2581 ggcaggctcg attttctcgg cttccggcaa ataccgtaat tgtctgcgca tcaactgcgc
     2641 tttgccgctc agcgaaacct atcgcgaagc actaaagcaa attggcgatg ccgtgtatcg
     2701 ggcaatggaa taaattcgaa ttagtgccga ggcccctgat ttactctcac gaataacctg
     2761 tgactcgttt tatctttata gcgacatcgc cacgttccgg catggcttag gcaaagtgtg
     2821 cttcgttcat gccagatgcc atgtgaacgc cttaccaggt ctacaaaata ttaaaagttc
     2881 aatatattta atggattatg tagatacctg ataagcgtag cgcatcaggc tgctttgcgt
     2941 ttgtcatcag cctctgatta gtactcgatc catggttcgc gcttgctttc aggcaagggc
     3001 tgatctaatg cctgcatttg ctctgccgaa agtccggtat atcgttgtat ccattcagga
     3061 tcggccccat tctgcaacaa caatcggagg aggctccgtt ccccttctac tttcccttct
     3121 ttcctccccc tctcccttcc caacagccat ccatcattat gaattcgctc tgcaattgtc
     3181 attattcgct ccctgtggtg tggcattcgc ctggtaagtt cgctgataaa cgccttaaaa
     3241 cgcgcttcat cgccagtcag taaaatgtaa tttaacagcg cagttatctg gctgtcatta
     3301 gcacactctg taaccagtaa tactaccagt tggtcaataa gccccatcag atcgcgctgg
     3361 cgaatatgct tttggatcaa ctccaacagg gcgactctgc gatgctgcac aatctcgtcg
     3421 tctggcacga cagtgacatc caccagcggg aacgctgcgc tataaagttt ccgtgcggta
     3481 gtcggggcgg caaattcgtc cagccaacac agggaccagg ggtaaggact acggctaccg
     3541 tgataaaaca gcatcgggat aaccagcggt agcggccggc gtttatcatg ctcgatatgg
     3601 cgctgcatca ccgccatgga atatcgcatc aggcgaaacg ccatatgaat atcctcgcgg
     3661 ctctgatgtt caatcactac gtaaatataa ccatcacctt cacgggtctt taccgaccac
     3721 agaatatcgg agtgtagcgc ccgcaatttt tcatcgacaa agctggcgga ttccagtttt
     3781 aagctgtcga gatcgcacag ttcacgtaaa tctttaggta agtggatctc cataaaatcc
     3841 cgcgcggtgc cagggtgcgt gagaaaggac ttaaataatg cgtcatgcgg cgtgctggtc
     3901 gagaagtttg tcatggcgat ccgtcacctg tagataagat ggcccgactt tagcggctaa
     3961 caaaaccatt ctcacccggc aatttcccgt tccccgaggc gctttccagg ctatttttat
     4021 tcttctgcaa acgatacatt ttttcggaaa caggctcgcg ttttacattt cctgtacccg
     4081 gcagatattc ccctttccac cgcgctcttt tgattaaccg caaattttaa ttacacttaa
     4141 ggtgtatatt ttctatgcaa cccatcaatt aaagaggtgt aatgtgctga tgactatttg
     4201 taatcgttat acattctgac ctggagtcag aaggtatttc tctgtctgtg tgttcacagg
     4261 cagtgtggtt gattacatga aattcagtac atttgtcctt ctcgcctgcc tttcgtcatt
     4321 accgacggta ttgaatttcg ttttccccgt tggggttctc cggacaagga gttgtttgtt
     4381 atgccacgtt tttttgcccg ccatgccgcc acgctgtttt tcccgatggc gttgattttg
     4441 tatgactttg ccgcgtacct gtcgacggat ctgatccagc cggggatcat taatgtggtc
     4501 cgtgatttta atgccgatgt cagtctggcc ccggcagccg tcagtctcta tctcgcgggc
     4561 ggtatggcgt tacagtggct gctggggccg ctttccgaca ggattggccg caggccggtg
     4621 ctgattaccg gggcgctaat atttaccctt gcctgcgctg cgacaatgtt cacaacgtct
     4681 atgacacagt ttcttatcgc gcgtgcaatt cagggcacca gtatctgttt tattgccacc
     4741 gttggttatg tcacggtgca ggaggcgttc ggccagacaa aagggatcaa gttgatggcg
     4801 attatcacct ccatcgtact gattgcgccg attattggcc cgctttccgg cgcagctctg
     4861 atgcactttg tgcactggaa agtccttttt gccatcattg cagttatggg ttttatctca
     4921 tttgttggct tactgctggc gatgccagag acggtgaagc gcggcgcggt tccatttagc
     4981 gccaaaagcg tctttcgcga ttttcgtaat gtcttttgca atcggctgtt cctctttggc
     5041 gcagcaacca tctctttaag ctatatccca atgatgagtt gggtggctgt ctcgccggtg
     5101 atcctgatcg acgcgggcgg cttaacaact tcgcagttcg cctggacaca ggttccggtg
     5161 ttcggcgcgg tgattgtcgc gaatgccatc gtggcgcgtt tcgttaaaga tccgaccgaa
     5221 ccgcgcttta tctggcgtgc tgtacccatt caactggtcg gcctcgcact gttgattatc
     5281 ggcaatctgc tgtcaccgca cgtctggctg tggtcggtgc tgggcaccag tttgtatgct
     5341 ttcgggattg gtttgatctt cccgacctta ttccgcttta cgcttttttc caataactta
     5401 ccgaaaggga ccgtctccgc atcgctgaat atggtgatcc tgatggtgat gtctgtctcg
     5461 gtcgaaatcg gccgctggct gtggtttaac ggcggtcgct tgccgtttca tctgttagcc
     5521 gtcgtggcgg gcgttatcgt cgttttcacc ctggcaggat tgcttaatcg cgtgcgccaa
     5581 catcaggctg ccgagctggc ggaggagcgg tgatttttgc gcgatcctgc cgtcaggctc
     5641 tattcttaac gttatgaata aactcgttga actcagacgc gccaaaatgt tggcgctctc
     5701 tttactgctt atcgccgctg ctacctttgt cgttacgctg tttttgccgc ccaatttttg
     5761 ggtgagcggc gtgaaggcga ttgctgaagc ggcgatggtc ggcgcgctgg cggactggtt
     5821 tgcggtggtg gcgctgtttc gccgcgtgcc gattccgatc atttctcgtc atacggcgat
     5881 tatcccgcgt aataaagacc ggattggcga aaatctcggc cagttcgtgc aggaaaaatt
     5941 tctcgatacc cagtcgctgg tggcattgat tcgacgtcac gaaccggcgt tgctgattgg
     6001 caactggttt agccagccag aaaacgcccg ccgcgttggt cagcatctgt tgcagatcat
     6061 gagtggtttt cttgaactga ccgatgatgc gcgtattcag cgcctgctta agcgcgcggt
     6121 ccatcgggcg attgataagg tcgatctttc cggaaccagt gcgttgatgc tggagagtat
     6181 gaccaaaaac gatcgtcatc aggtgctgct cgatacgctg atcgcacagt tgatcgccct
     6241 tctccagcgc gataaatcgc gcaagtttat tgcccagcag attgttcgct ggctggagag
     6301 tgagcatcca ctgaaagcca aaattctccc caccgaatgg ttgggcgaac atagcgcgga
     6361 gttggtttct gacgcggtga attctttgct tgatgatatc agccgtgatc gtgcgcatca
     6421 gatccgtcat gcgtttgatc gcgccacttt tgccctgatc gacaagttga aaaacgatcc
     6481 ggaaatggca gcgcgagccg atgccgtaaa aagttatctg aaagaagatg aagcttttaa
     6541 ccgctatctc agtgaattgt ggggggattt acgggagtgg ctgaaagcgg atatcaacag
     6601 tgaagattct cgtgtgaaag aacgtatcgc gcgggcgggt caatggtttg gcgaaacgtt
     6661 aattgccgat gatgccttgc gggcgtcgtt aaatggtcac ctggaacaag ccgcgcaccg
     6721 cgtcgcgcct gagttttccg cattcctgac gcgccacatc agcgatacag taaaaagctg
     6781 ggatgcgcgg gatatgtcgc tgcaaatcga gttaaatatc ggcaaagatc tgcagtttat
     6841 ccgtgtcaac ggtacgctgg ttggcggttg tattgggcta attttatatt tgttgtcgca
     6901 gctcccggcc ttgttccccc tcggcaattt ttagaaatcc atgaaaaact aagtggctaa
     6961 ccgcgagaga gatcaaataa accacactaa aagaatggta ataatgtggc ctcttttcat
     7021 tatctcccgt ggtacgggga aggaaaatca tgtcacttat caccgatcta cccgccattt
     7081 tcgatcagtt ctctgaagct cgccagaaag gctttctcac cgtcatggat ctcaaggagc
     7141 gcggcattcc gctggttggc acttactgca cctttatgcc gcaagagatc ccgatggcag
     7201 ccggtgcggt tgtggtttcg ctctgttcta cctctgatga aaccattgaa gaagcggaga
     7261 aagatctgcc gcgcaacctc tgcccgctga ttaaaagcag ctacggcttc ggcaaaaccg
     7321 ataaatgccc ctacttctac ttttcggatc tggtggtcgg tgaaaccacc tgcgacggca
     7381 aaaagaaaat gtatgaatac atggcggagt ttaagcccgt tcatgtgatg cagttgccga
     7441 acagcgttaa ggacgatgcc tcgcgtgcgt tatggaaagc cgagatgctg cgcttgcaaa
     7501 aaacggtaga agaacgtttt gggcacgaga ttagcgaaga tgctctgcgc gatgccattg
     7561 cgctgaaaaa ccgcgaacgt cgcgcactgg ctaattttta tcatcttggg cagttaaatc
     7621 ctccggcgct tagcggcagc gacattctga aagtggtcta cggcgcaacc ttccggttcg
     7681 ataaagaggc gttgatcaat gaactggacg cgatgacagc ccgcgttcgt cagcagtggg
     7741 aagaaggcca gcggctggcc ctgcgtccac gcattttaat caccggctgc ccgattggcg
     7801 gcgcagcaga aaaagtggtg cgcgcgattg aagagaatgg cggctgggtt gtcggttatg
     7861 aaaactgcac cggggcgaaa gcgaccgagc aatgcgtggc agaaacgggc gatgtctacg
     7921 acgcactggc ggataaatat ctggcgattg gctgctcctg tgtttcgccg aacgatcagc
     7981 gcctgcaaat gctcagccag atggtggaag aatatcaggt cgatggcgta gttgatgtga
     8041 ttttgcaggc gtgccatacc tacgcggtgg aatcgctggc gattaaacgt catgtgcgtc
     8101 agcagcacaa cattccttat atcgctattg aaacagacta ctccacctca gatgtcgggc
     8161 agctcagtac ccgtgtcgcg gcctttattg agatgctgta aggagtggca gtgacatatt
     8221 cgattggcat tgattctggt tctaccgcca ccaaagggat cttactggca gacggcgtga
     8281 ttacgcgccg tttcctcgtt ccaacaccct ttcgtccggc aacagcaatt actgaagcct
     8341 gggaaactct gcgcgaaggg ttagagacaa cgccgtttct gacgctcacc ggctatggac
     8401 ggcaactggt ggattttgcc gataaacaag tgacggaaat ctcctgtcat gggctgggca
     8461 cacggtttct tgcgccagca acgcgcgcgg taatcgacat cggtggtcag gacagcaaag
     8521 tgattcagct tgatgatgac ggtaacctgt gcgatttcct gatgaatgac aaatgcgcgg
     8581 cgggcaccgg gcgtttcctg gaggtgatct cgcgcacgct tggcaccagc gtcgagcaac
     8641 tcgacagcat taccgaaaat gtcacgccgc acgccatcac gagtatgtgc acagtgtttg
     8701 ccgaatcaga agtgatcagc ctgcgctcag cgggcgtcgc gccagaagcg attctcgccg
     8761 gagtgattaa cgcgatggcg cggaggagtg ccaatttcat tgctcgtctc tcctgtgaag
     8821 cgccgattct gtttactggt ggcgttagtc attgccagac gtttgcccgg atgctggaat
     8881 ctcatctggg aatgccggta aatacccatc ctgatgcgca atttgctggc gcaattggcg
     8941 cggcggtaat tggtcaacga gtgaggacac gccgatgaaa gagtttttat ttttatttca
     9001 ctccacggtc ggcgtcatac aaacccgcaa ggcgttgcag gcagcgggca tgacttttcg
     9061 cgtcagcgat attccccgtg atttacgcgg cggctgcggg ttatgcattt ggctgacctg
     9121 tccccccggc gaggaaatac aatgggtgat ccctgggcag actgagtcaa tttattgcca
     9181 gcaggatggt gtctggcgct gcattgcaca ttacggggtt tctccgcgtt aaggattcat
     9241 taaggtcgct acgtatactt ctcgatatct tattgatttg ccttaaaggg attaccgtga
     9301 caaaaagtat ttcgttaagt aaacgcatct ccgttattgt gattcttttt gccattgtgg
     9361 cagtgtgtac tttcttcgtg caatcttgcg cccgtaaaag caatcatgca gcatcatttc
     9421 agaattacca cgccaccatt gacggcaaag aaattgcagg cataacgaat aatatctctt
     9481 cgctgacctg gtcggcacaa agcaatactc tttttagcac tatcaacaaa cctgccgcca
     9541 ttgttgaaat gaccaccaat ggtgatttta tccgcacaat tcctctggat tttgttaagg
     9601 atctcgagac gatcgaatac attggcgata atcagtttgt cattagcgat gagcgcgatt
     9661 acgccattta tgtgatttca ctgaccccaa actcggaagt aaaaatcctc aaaaaaataa
     9721 aaatcccgct gcaagactcc ccaaccaatt gcggttttga agggttggct tattctcgcc
     9781 aggatcacac tttctggttt tttaaagaga aaaatccgat tgaggtctac aaagttaacg
     9841 ggctgttaag tagcaatgaa ttacacatca gcaaagacga ggcactacaa cggcaattta
     9901 ctttagatga tgtgtccggc gcagaattta accagcaaaa aaatacgctt ctggtgctgt
     9961 ctcatgaatc tcgcgcactc caggaagtaa ccctggttgg cgaggttatc ggtgaaatat
    10021 cactaaccaa aggtagtcga ggtttgtcgc ataacatcaa acaggctgaa gggattgcga
    10081 tggatgcctc tggtaatatt tatatcgtta gcgaaccaaa tcgtttttat cgctttaccc
    10141 ctaaatcatc gcattaacag gctggcatat ttcacctttt tgttattaac ttgttaacaa
    10201 caaaaatgac atacaaggtt aaacatgcct tcgtcctcgc atcccgtaga acgcttttct
    10261 ttcagcaccg cgcttttcgg gatgctggtt ctgaccttag gtatgggatt aggtcgcttc
    10321 ctttatacgc ctatgttgcc cgtcatgatg gcggaagggg cgttttcatt tagccagctc
    10381 tcgtggattg ccagcggcaa ctatgcgggg tatctggctg gcagtctgct attttcgttt
    10441 ggcgcatttc accagccgtc gcgcctgcgc ccatttctgt tggtttctgc cctagcgagc
    10501 gggttgttga tcctcgcaat ggcatggttg ccgccattta ttctggtgtt attgattcgc
    10561 ttcctggcgg gtgtcgccag cgccggtatg ctgatttttg gttcgacgct gattatgcag
    10621 cacacgcgcc atccttttgt gctggcagct ttgttttctg gcgttggcat tggcattgca
    10681 ctgggcaacg aatatgttct ggcaggcctg cattttgcct tatcctcgca aacgttatgg
    10741 caaggcgccg gggcgctttc tggcatgatg ctgattgcac ttacgctttt aatgccctcg
    10801 aaaaaacacg ccatcgcacc aatgccattg gcaaaaacgg agcaacagat aatgagctgg
    10861 tggttactgg ctattctgta cggcctggcg ggatttggtt atatcatcgt cgcgacttac
    10921 ctgccgctca tggcgaaaga tgcaggctca ccattgttaa ccgcccatct ctggacgtta
    10981 gtcggcttat caattgtgcc aggctgcttt agctggctat gggcagcaaa acgttggggg
    11041 gctttgcctt gcctgacggc gaatttgctg gtgcaggcta tcagtgtgct gcttactctc
    11101 gccagcgact ctcctctctt gcttatcatt agtagtatcg gttttggcgg tacctttatg
    11161 ggcacgacat ctctggtgat gactatcgcc cgccaactga gcgttccggg aaatctcaat
    11221 cttttaggct ttgtgacgct catttacggc attggacaaa tcctcggccc ggcgctgacc
    11281 agtatgctcg gcaatggaac atcggcgctt gccggcgcca ctctctgcgg cgcggcggcg
    11341 ttgtttattg ctgcgcttat cagcacagta caattattta agctgcaagt ggtcacttca
    11401 tgaggagtgt ttcccttacc actcaagaaa ttcgaccgca accgttgatg tcagccagac
    11461 tccgttttcc gcttgccaga aagggatgcc tcgtttcgcc atttcctgtg ctttgacggt
    11521 taagataacc ggcgatccgt gtcttgctcc cactttgcgt gccgttgctt catcagcaga
    11581 gagatgcacg taatgacgct cccctgcaat cagcccctgt ttttttatct cgtcaaggaa
    11641 acggcttgcc gtaccgtggt aaagaaactg tggcggtgtt ttttcggcaa aggaaatggc
    11701 gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac ctaccatcac tggaataact
    11761 aaaacgcttt ttatcactgg tagcgaccac ggtatccagc aatgcacggg ttaaccgctt
    11821 gcccgctttt tgggcacaga gtataagctt atcgatatcc gcccatcctt cacggtcaag
    11881 tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa cttaaaaact tgctcgtatc
    11941 cgccaactct ttttcgttgt actttgccat ctcgttttcg tccctggatt ttctgttttt
    12001 atttactgat gtcattgtca ctgggtttat gaattttctg caactcaatc ctgcgcagag
    12061 ccttttccac acagcataat ttttccgcta tgcaaaaaac gcatagccag aaacatactt
    12121 gcattttccc catacccaca tggctgataa ggtttttccg ctccaggaat tttcctggct
    12181 caagcaacac acaaacacaa aaaaacaatg gggattgtta tgacacaaca aggggatgct
    12241 gtagccgggg agctggcgac agaaaaagtg ggcattaaag ggtatctggc tttttttctc
    12301 accataatct ttttttccgg tgttttttcc ggaacagaca gctggtggcg cgtttttgat
    12361 ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg cgaacggagc caccacctca
    12421 tttcgcgggg cgggtggcgc gggggctaaa gatggctttc tctttgcgct ggagctggca
    12481 ccgtcagtca ttctctctct ggggattatt tcaattaccg atggtcttgg tggattacgc
    12541 gccgcgcagc agttaatgac gcctgtgcta aaaccgttgt tgggcattcc agggatctgc
    12601 tcgctggcgc tgatcgctaa cctgcaaaac acggatgccg ccgccggtat gaccaaagag
    12661 ctggcgcagg aaggggaaat taccgaacgc gataaagtga ttttcgccgc atatcaaacc
    12721 agcggtagcg ccatcatcac caactacttc tcttccggcg tcgcggtgtt tgcctttctg
    12781 ggcacgtccg taatcgtacc tttagccgtc atcctggtgt ttaaatttgt cggagccaac
    12841 attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc caacgcaagg agcgcaagca
    12901 tgacaactca ggtccgcaaa aatgtcatgg atatgtttat cgatggtgcg cgccgtggct
    12961 ttactatcgc cacgaccaac ctgctgccaa acgtggtgat ggcgtttgtc atcattcagg
    13021 cgctgaaaat taccggcctg ctcgactggg tggggcatat ctgtgaaccc gtaatggcgc
    13081 tgtggggatt acccggtgaa gcggcaaccg tccttttagc cgcattaatg agtatgggcg
    13141 gcgcagtcgg tgttgccgcc agtctggcga ccgccggcgc attaaccgtt cacgatgtca
    13201 ccgttttact gcccgcgatg tatttaatgg gcaacccggt gcaaaatgtc ggtcgctgcc
    13261 tggggacagc cgaagtgaat gccaaatatt acccgcatat cattacggtc tgcgtgatta
    13321 acgcattgct gtcgatctgg gttatgcagc ttattgttta acaaggagtt accatgattg
    13381 attataccgc agccggtttt accctgctgc agggagcgca tttgtatgcg ccggaagatc
    13441 ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat cgccgttgcc agcaatatcc
    13501 cttctgacat tgtaccggac tgcacggttg tcgatctcag cgggcagatc ctctgtcctg
    13561 gttttattga tcaacacgtc catttgattg gtggtggcgg cgaagcaggt cccacgacgc
    13621 gcacgccaga agtggcgcta agtcgcctga cggaagcggg cgtcacgtca gtggttggtc
    13681 tgctgggcac cgactctatc tctcgccacc cggaatccct gctcgccaag acccgtgcgc
    13741 tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc ttatcatgtc ccttcccgca
    13801 ccattacagg ttccgtggaa aaagacgtgg cgattatcga tcgtgtgatt ggcgtgaaat
    13861 gcgccatctc tgatcaccgt tctgccgcac cggacgttta tcacctggct aatatggcgg
    13921 cggaatcccg cgttggcggt ttgctcggcg gtaaacctgg cgtcaccgtg ttccacatgg
    13981 gcgacagcaa aaaggcgtta cagcctgtct atgacctgct ggaaaactgc gatgtgccga
    14041 tcagcaagtt gctgccgacc cacgttaacc gcaacgttcc gttgtttgag caggcgctgg
    14101 agttcgcgcg caaaggcggc actatcgata tcaccagcag cattgacgaa ccggtcgccc
    14161 ctgccgaagg tattgcccgc gccgttcagg cgggtattcc gctggcacgc gtcaccctca
    14221 gctccgacgg caacggtagc cagccgttct tcgatgacga agggaattta acccatatcg
    14281 gtgttgccgg ttttgaaacg ttgctggaaa ccgtgcaggt gctggtcaaa gactatgatt
    14341 tcagtatcag cgatgccctg cgcccgctca ccagtagcgt agccggtttc cttaacctga
    14401 ccgggaaagg cgaaattctg ccaggcaatg atgctgactt gctggtcatg acgccagaac
    14461 tgcgcattga gcaggtatac gctcgcggca aactgatggt caaagacggc aaagcctgcg
    14521 tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg cccgaatgga cgtagaatgc
    14581 ccgcaagggc ggctgacaga gtaaaacgta atggatgact gtggtgcgat tttgcataat
    14641 attgaaacca aatggcttta tgattttctg cccctggaaa aatgccgcaa tttttcccag
    14701 gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc gccgcatccg tgcgctggaa
    14761 caggcgattg gtgttgaatt gtttaaccgc caggtaacgc cgctgcaact ctcagaacaa
    14821 gggaaaatct tccattcgca gatccgccat ctgttgcaac agttagagag caatctggca
    14881 gagctgcgtg gcggcagcaa ttacgcgcaa cgaaaaatca agatagccgc tgcacactct
    14941 ctttccctcg ggctgttacc gtccattatc agccagatgc cgccgctctt tacctgggca
    15001 attgaagcta ttgatgtcga tgaagcggtc gataaactgc gtgaagggca aagtgactgt
    15061 attttttcct ttcacgacga agatttgctg gaagcgccgt ttgaccacat tcgcttattt
    15121 gaatctcaat tgttccctgt ctgcgccagt gacgaacacg gagaagcact ttttaacctc
    15181 gcgcagccac actttccgtt actgaattac agccgcaact cctacatggg gcgattgatt
    15241 aatcgcaccc tgacgcgcca cagtgagtta agtttcagca ccttttttgt ctcttcgatg
    15301 agcgagcttt taaagcaggt tgccctcgac ggctgtggga ttgcctggct gccggagtac
    15361 gccatccaac aagaaattcg cagcaggaag ctcgttgtac tgaatcggga cgaactggtg
    15421 atcccgattc aggcctatgc ataccgaatg aatacccgca tgaatcccgt tgccgaacgc
    15481 ttctggcgtg aactgcgcga gctggagatt gtacttagct gacattctcc agcgtcgcac
    15541 tacgccaacc gccattatcc cagaccagat caaacgttag cggttcatcg tcgtggtaca
    15601 gcattatctc aaggctgatc gccagcgtgt gactgtacgc ttctttcaga ttgacctgca
    15661 acgccgcaat ccgtggctca tgaaataaca gtgcagaacg cagctgacgg caataccagc
    15721 tctggagatg tttgtcgctc tggttatgca cttgccactc gggcatgcct gaaacaagta
    15781 atgacggtaa tgccgaaaga ggagaattaa aaagagtgag gagatcgctg cataccgatt
    15841 tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa aacagcctga agtgattggc
    15901 gcatcatttt gcgcactcct gacgtttagc aacatatgcg gcttgccctg aacaccgggc
    15961 accactgtat tgatgctcat tttccatagc cattcccttt tgttcattac atccatgtca
    16021 gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac cttacttgcc tatgaatatc
    16081 tactgacttt tcaggaaaaa agtcatgatt tgataaaaat catcaaactc aaaaaaaaac
    16141 cgctagtata ttctgaatat tccgaatagt tatgtagatg aaaatacttg tcaccgccgc
    16201 tacttaat
//
LOCUS       DAZGTR010000098        15978 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGTR010000098 DAZGTR010000000
VERSION     DAZGTR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.099"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..210
                     /locus_tag="ACHLCY_003524"
     CDS             <1..210
                     /locus_tag="ACHLCY_003524"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370704.1"
                     /translation="IIYSLLNSRRGSRHEATGEFPFACYPASLSATLSGIADCAIPHF
                     LFNKLKPVFISGMNVGIEPIIGVKG"
     gene            532..804
                     /locus_tag="ACHLCY_003525"
     CDS             532..804
                     /locus_tag="ACHLCY_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP0370705.1"
                     /translation="MALPRIPQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(805..1677)
                     /gene="rluF"
                     /locus_tag="ACHLCY_003526"
     CDS             complement(805..1677)
                     /gene="rluF"
                     /locus_tag="ACHLCY_003526"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP0370706.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            2026..2974
                     /locus_tag="ACHLCY_003527"
                     /pseudo
     CDS             2026..2974
                     /locus_tag="ACHLCY_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            3044..3850
                     /locus_tag="ACHLCY_003528"
     CDS             3044..3850
                     /locus_tag="ACHLCY_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0370707.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3860..4267
                     /locus_tag="ACHLCY_003529"
     CDS             3860..4267
                     /locus_tag="ACHLCY_003529"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFP0370708.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            4267..4761
                     /locus_tag="ACHLCY_003530"
     CDS             4267..4761
                     /locus_tag="ACHLCY_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP0370709.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4827..5624
                     /locus_tag="ACHLCY_003531"
     CDS             4827..5624
                     /locus_tag="ACHLCY_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFP0370710.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEAWFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            5635..6459
                     /locus_tag="ACHLCY_003532"
     CDS             5635..6459
                     /locus_tag="ACHLCY_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP0370711.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6511..7740
                     /gene="sorE"
                     /locus_tag="ACHLCY_003533"
     CDS             6511..7740
                     /gene="sorE"
                     /locus_tag="ACHLCY_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFP0370712.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            8123..8812
                     /gene="pepE"
                     /locus_tag="ACHLCY_003534"
     CDS             8123..8812
                     /gene="pepE"
                     /locus_tag="ACHLCY_003534"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP0370713.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8903..10534)
                     /locus_tag="ACHLCY_003535"
     CDS             complement(8903..10534)
                     /locus_tag="ACHLCY_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP0370714.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10754..14437)
                     /gene="metH"
                     /locus_tag="ACHLCY_003536"
     CDS             complement(10754..14437)
                     /gene="metH"
                     /locus_tag="ACHLCY_003536"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP0370715.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14637..15461
                     /gene="iclR"
                     /locus_tag="ACHLCY_003537"
     CDS             14637..15461
                     /gene="iclR"
                     /locus_tag="ACHLCY_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP0370716.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15778..>15978
                     /locus_tag="ACHLCY_003538"
     CDS             15778..>15978
                     /locus_tag="ACHLCY_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370717.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKD"
BASE COUNT         3857 a         4151 c         4134 g         3836 t
ORIGIN      
        1 attatctatt ccttgcttaa cagccgtcgc ggaagcagac acgaagcgac aggggaattc
       61 ccttttgcct gttacccggc atctttatcg gcgacgttat cggggatcgc tgactgcgca
      121 atccctcact ttttatttaa taagttaaaa ccagtattca tttcaggaat gaatgtggga
      181 atcgaaccca taattggcgt caaaggctga cggaacacgc ccggaaaaca gcctggccgt
      241 tcctggtgtg ccgacggggt ttgaccccct ttgtaattca gtttcaacgc gtacgagata
      301 ttgcgagaag cgtgccagaa ttgagaaaaa attattaacc aaatgaaaaa taaagattta
      361 ataaattatt tttgtttgta gagggaaaac ttttcgctaa atttgtatct gtatttatcg
      421 tcaattatca agaaagataa atatttaaga agagaacaaa ggaatattat cagttcagct
      481 cgcaaacgaa ttcccgtaca ctgaactctc tggctcactg agggcaccat catggcactc
      541 ccccgtattc cccaaaaaga gatgactgaa cgcgagcagc gtgaacttaa aacactgctg
      601 gatcgggcgc ggattgcgca tggtcgcgta ctgaccaact ccgaaaccaa cagcattaag
      661 aaagagtaca tcgataaatt aatggtcgag cgtgaggctg aagcgaaaaa agcccgccag
      721 ttgaagaaaa agcaggctta taaaccggat cctgaagcgt catttagctg gtcagcaaat
      781 acgtcgacgc gcggcaggcg ctgattagcg tcccttcttt ttacgccccg gcgaggtaaa
      841 acgcttaccg ttggacgccg ggcgaccgcc tttttccgcc gttttttcca tcttaacgac
      901 tggacgtttg atgcccgctg ttttcggttt agccttcgct ttaggtttta cctcggaaga
      961 ggaattttca atgagtttaa acaggtcgat taactcatcg tcagttaaat cgcgccactc
     1021 acccagcgga atgccgctta aactgacgtt cataatgcgc gtacgttcca gctttttcac
     1081 ttcatagccg aaatgctcgc acatgcgccg gatctgacgg ttcagcccct gcaccagggt
     1141 aatgcggaag acaaacggcg cttctttttt gactttgcac tttttcgtca ccgtcccgag
     1201 gatcggcacc cccgccccca taccacgaat aaattcatcg gtaatcggtt tatcgaccgt
     1261 caccagatac tctttctcat gatcgttacc cgcacggagg atcttattca ccagatcgcc
     1321 gtgattggtg aggaaaatca gcccctggga gtctttatcc aggcggccaa tcgggaacac
     1381 gcgtttgctg tggttaacga aatcgacaat gttatcgcgc tcgccatctt cggtggtgct
     1441 gacaataccg acgggcttgt tcagggcgat aagtaccaaa tcttcggctt cccgaggctc
     1501 aatcaactga ccgtttactt tcacaatgtc gccgggtttc acctgatcgc caatggtggc
     1561 tcgcttgcca ttaaggaaca cattgccttg ctcgatatag cgatccgctt cgcggcgtga
     1621 gcaaattccg ctttcgctga tgtatttatt taaacggact gatgagtcgg gcagcataaa
     1681 ttctcctgta aaacggcgga atataccgta cctcttagcg gggaaaaaca gggttatgcg
     1741 gcaacgtcga aattaaatgt gatctgccac gccttttatc acaaaagtgt ctccggaagt
     1801 acgatgcaaa tttgcctggc aaaataagga taatcaacat attacgaatc gctgcacacc
     1861 tgtgctgatg actactacgc agagaaacaa atgtgcaaga gaaagcgcta tttctcccca
     1921 tgcaaagagt gaaaaatcgc tttgaaaggc cctcatgatg gggttatatc agatcaagcg
     1981 gtgagaaagc gtatttgcac aaatgtgcag aaccaggagg cggcaatgga aaacagtgac
     2041 gatatccgtt tgattgtgaa gattgcccaa ctctattacg aacaggatat gacgcaggcg
     2101 caaatcgcgc gcgaactgcg tatttaccgc accaccatca gccgcttgct taaacgaggc
     2161 cgcgatcagg gaattgtcac catcgccatc aactatgact acaacgaaaa tctctggctg
     2221 gagcagcaac tgaagcaaaa gtttggcctg aaagacgttg tggtggtgtc gggaaatgat
     2281 gaggatgaag agactcaact ggcgatgatg gggttacacg gcgcgcaact gctggatcgc
     2341 ttgctggagc ctggcgatat tgtcggtttt tcctggggtc gcgcggtgag cgcactggtt
     2401 gaaaacttgc cgcaggcggg gcaatcgcgg cagttaatct gcgtaccaat tattggcgga
     2461 ccgtccggta aactcgaaag ccgctatcat gtaaacacat taacctacag cgcggcagcg
     2521 aagctgaaag gggaatcgca tctcgcgtat tttccggctc tgctggataa cccattaatt
     2581 cgtaatggga tcatgcagtc tcagcacttt aaaaccatct ctgcctactg ggataatctg
     2641 gatgtcgccc tggtgggaat tggctcaccg gccattcgcg acggcgctaa ctggcatgcg
     2701 ttttatggtg gtgaagagag tgacgacctg aatgcccgcc aggttgctgg cgatatttgc
     2761 tcgcgctttt ttgatattca cggcgcaatg gttgaaacga atatgagcga aaaaacgctc
     2821 tctatcgaaa tgaataaatt aaagcaggcg cgatattcca ttggcattgc catgagtgaa
     2881 gaaaaataca gcggaattat tggttgcact gcgtggaaaa tatattaatt gtctggtaac
     2941 gaatagcagc acagctgaac tattactgaa ataaaaatct tcccgatgtt taccgtaata
     3001 aaaattacgg cgggtcccta ttatctaaaa tcaggagtct gttatgcaaa cgtggttaaa
     3061 tttgcaggat aaaatcatta tcgtcaccgg cggcgcatcc ggtattggtc tggcgattgt
     3121 agaggaatta ttagcacaag gcgcaaacgt acagatggta gatattcacg gtggcgatgg
     3181 tcaatatgaa ggtcataaag gctatcagtt ttggccgacc gatatttcca gcgccaaaga
     3241 ggtaaatcat acggtagcgg aaattatcca gcgttttggt cgcatcgacg gtctggtcaa
     3301 taacgccggg gtcaatttcc cgcgtctgct ggtcgatgag aaagcgcctg ccgggcagta
     3361 tgaactcaac gaagctgcat tcgaaaaaat ggtcaatatc aaccagaaag gcgtttttct
     3421 gatgtcgcag gcggtggcgc gacagatggt caaacaacat gatggcgtga ttgtgaatgt
     3481 ttcctcagaa agtggactgg aaggctcaga aggccaaagc tgttacgccg ctaccaaagc
     3541 cgcgcttaat agcttcacgc gctcctggtc gaaagagctg ggtaaacacg gtatccgtgt
     3601 ggtcggtatc gcgcccggga ttctggaaaa aaccgggcta cgcacgccgg aatatgaaga
     3661 ggcgctggcg tggacgcgca atatcaccgt cgagcagctg cgtgaaggct ataccaaaaa
     3721 cgccattcct attgggcgcg ccggaagatt agcagaagtc gctgattttg tttgttatct
     3781 gttgtctgaa cgcgccagct acatcaccgg agtaaccact aacattgcgg gcggcaaaac
     3841 gcgcggctaa ggaggcttta tggtcaacgc cattttttgt gcccacggca aattagcttg
     3901 cgccatgctg gaatcggttc agatggttta cggcaacgcc aatgttgaag ccgttgaatt
     3961 tgtgcccggc gagaacgccg gagacatcgt agcaaagctg gaaaagttag taagcattca
     4021 caatcaagat gagtggctga ttgccgtgga tttacagtgc ggcagcccgt ggaatgcggc
     4081 agcgatgctt gcgatgcgca atcctcgtct gcgggtaatc agcggccttt ctctgccgct
     4141 ggcgctcgaa ctggttgata accaggacag catgaatgtt gatgaactgt gtgaacacct
     4201 gacgcaaatc gcaaagcaaa cctgcgttgt ctggaaacaa ctggcaacgg cagaggagga
     4261 tttctgatga acattacgct cgcccgcatt gatgaccgcc tgatccacgg tcaggtcact
     4321 accgtctggt cgaaagtggc taacgcccag cgcattatta tctgcaatga cgaagtttat
     4381 aacgatgaag ttcgccggac attattgcgt caggctgcgc caccgggaat gaaggttaac
     4441 gtggtcaata ttgaaaaagc cgtcgctgtt tatcacaacc cgcaatatca ggacgaaacc
     4501 gttttttatc ttttcactcg cccacaggat gccttagcga tggttcgcca gggggtaaaa
     4561 atagacaccc tgaatattgg cggcatggcc tggcggcccg gtaaaaaaca gttaaccaaa
     4621 gcggtttcat tagatgatga tgatattaac gcatttcatg agttaaataa tcttggcgtc
     4681 atactggatt tacgcgtcgt agcgtcagat ccctcaatca atattatcga caaaataaac
     4741 gaacagttaa tcgcaaatta aataatagcg cctgtgtaca tatgctttaa caggcaggga
     4801 tgacgacact taaaaggtga aaaatcatgg aaataagtac cctacaaatc attgctatat
     4861 ttcttttttc ctgtattgcc ggaatgggca gcgtgctgga tgaatttcag actcatcgcc
     4921 cgttaattgc ctgtacggtg attggtttaa ttctcggtga tttaaaaacc ggaataatac
     4981 tcggtggtac gctggaattg atagctctcg gctggatgaa cgtcggcgcg gcgcaatctc
     5041 cggattctgc actcgccagc ataatctccg ccattctggt tatcgttggt cagcaaagca
     5101 tcgccaccgg aatcgccatc gcgttgcctg tggctgcggc aggccaggtg ctgactgtgt
     5161 ttgcccgtac catcaccgta gtgttccagc acgcggcgga taaagcagcg gaagaagcat
     5221 ggtttcgcac tctcgatatt ctgcatgtct ccgcgcttgg cgtgcaggcg ctgcgcgttg
     5281 ctattccggc actgattgtc tcactgttcg tcagcgccga tatggtgagc aatatgctga
     5341 gcgcgatacc ggaattcgtg acgcgcggtc tgcaaattgc tggcggtttt atcgtggtgg
     5401 tcggttacgc catggtgcta cgtatgatgg gcgtgaaata tttgatgcct ttctttttcc
     5461 tcggtttcct cgcaggtggc tacctcgatc tcagtctgct ggcgttcggt ggcgtcggcg
     5521 tgatcatggc cctggtctac atccagctaa atccacagtg gcgtaaagct gaacctcaac
     5581 cccaggccac cacctccacc gcccttgacc aacttgacga ttaacggagc catcatggaa
     5641 cagagaaaaa ttacacgcag cgatctggtg agcatgtttc tgcgctccaa cctgcaacag
     5701 gcgtccttta actttgaacg tattcacggg ctgggctttt gctacgacat gatccccgcc
     5761 atcaagcgac tttacccatt aaaagaggat caggttgcgg cgctcaggcg acacctggtg
     5821 ttcttcaata ccacgccagc cgtatgtggc ccggtcatcg gcgtcactgc cgccatggaa
     5881 gaggcgcggg ccaacggcgc ggaaattgat gacggtacca ttaacggcat caaagtcggt
     5941 ctgatgggac cattggcagg ggttggcgat ccactggttt ggggaacgct gcgcccgatt
     6001 accgctgcgc tcggcgcatc tctggcactt tctggcaaca tcctcggccc gcttttgttt
     6061 ttctttattt tcaatgcggt gcgtctggcg atgaagtggt atggcctaca gctcggcttt
     6121 cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa actcaccgaa
     6181 ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tcaccaaatg gacgtcaatc
     6241 aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt caccatgacc
     6301 gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct gacgctgctt
     6361 atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc cctgtttggc
     6421 ttagggatta tcggtaatgc gctgggcttc ctgtcctgat tcttcgcccc ggcacgactg
     6481 ccggggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg tctttatggt
     6541 aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga tgaaatcctc
     6601 gcgacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa tctgggtgaa
     6661 aaccataaaa aagtacccga cgatgtggcg accaacccca tcatcatcgg ccacgagttt
     6721 tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc gggtcagcgt
     6781 tatgtgattc aggccaacct gcaactcccc gaccgcccgg actgccccgg ctactccttc
     6841 ccgtgggtgg gcggcgaagc cacgcatgtg gttattccca acgaggtcat ggaacaagat
     6901 tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga accgctttcc
     6961 tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta taaccacacg
     7021 atggggattc gcccgcaagg gcgcatgctg atcctcggcg gcaccggacc aatgggactg
     7081 ttggcgattg attatgcgct acatggaccc gttaacccgt cgctgctcgt cattaccgat
     7141 accgacaacg ataaattgag ttatgcgcgc aagcactatc catcagaacc gcaaacactg
     7201 attcattatc tcaatgccgc cgatgcagca tttgatacgc taatggcgct gagtggcggt
     7261 cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac tctcgcctct
     7321 tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga taaacatttc
     7381 agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt gggcacgtca
     7441 ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga ttgaagagaa aaaagtgcag
     7501 gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac cacgcttgaa
     7561 ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct gccgctgacg
     7621 tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca tcaggggatc
     7681 tggtccggtg aggcggagca atatctgctc actcatgcag aggcaatttc ccatgattaa
     7741 tcacgctact taactctgca tgtcgctggc tggtcgccgc ggcaacttcg cgacctgatg
     7801 tgcggctgat tgctgaagcg gcccaatgtg cacgagaagg ataaaaaaac atcagcgcct
     7861 taaggggcgc tcaaattggt ggcaaagtac acattgttta tgccggatgc ggaataaagc
     7921 cttattcggc ctacataatc tcgaaaattc aatatattgc agttatctgg taggcctggt
     7981 aagcgtagcg catcaggcaa tgctgtgttt gtcatcagtc tcagcgcccg gtgcaatacg
     8041 tgacatcgtg agccatgtca gtaagtggtt aaattcgcag tggtagcatg cctcccgtga
     8101 tttcttacta actggagaag taatggaact gcttttattg agtaactcga cgctgccggg
     8161 taaagcctgg ctggaacatg cgctgccgct gattgctgaa cagttgcagg gtcgccgttc
     8221 agcggtattt attcctttcg ctggcgtaac gcagacctgg gatgattaca cagcgaaaac
     8281 ggctgcggtt ctcgctccgc tgggtgtatc tgtcaccggt attcatagcg ttgtcgatcc
     8341 cgttgccgcg attgaaaatg ctgagatcgt gattgtcggg ggcgggaata ctttccagtt
     8401 gctgaaacag tgccgcgagc gcgggctgct ggcaccaatt actgacgtgg ttaaacgtgg
     8461 tgcgctgtat attggctgga gcgcaggcgc taaccttgct tgcccaacca ttcgtaccac
     8521 caacgatatg ccgattgtcg atccgcaagg tttcgatgcg ctaaatctgt tcccgctgca
     8581 aatcaacccg cacttcacca acgcgctgcc ggaaggccat aaaggtgaaa cccgtgagca
     8641 gcgtattcgc gaactgctgg tcgtcgcgcc agaactgacg attattggtc taccggaagg
     8701 taactggatc acagtgagta aaggtcacgc tacgctgggt ggcccgaaca ccacttatgt
     8761 gtttaaggct ggtgaagaag cggtgccgct agaagctggt caccgttttt aagtaaattg
     8821 ccggatgcat gatccggcat cttgttcata gtgcctgatg cgacgcttga cgcgtcttat
     8881 caggcctaca ggattcacaa tattaatact cgtcccgtcc ttcatcttca tccggctgtt
     8941 ccagcacact gtaagccacc gaacaaaaca gcgagttcag gcgctgcata tctcccagta
     9001 agcctaaatg tagcgaactg gtttcaatgc tctgcacgtt ttgctgatgc aggcgatcga
     9061 cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaaacg atgtttgctg cgacgcaaac
     9121 gacgagcgct ggtgacatcg ccagagaaga acaccgacat tgccagcttt aaattactga
     9181 gcaattgctc atagagcgca tccagttcct tcaacccatc aagcgaaaat gcccgccgtg
     9241 ctgccagcga tttatcggca atttcgctgc ccatgcgctc gacgatatcg gaagcctgtt
     9301 caaggttgag cgacatttca ataatctccg cccagcgacg cgactcttct tctgccagtt
     9361 cctcttttgg catccgcgcc agatacagct taatggcggt atagagaacg ttgatatcat
     9421 ctgccagctt acgcagctct ttctcctgcc gtggctcgcc gtgcatcact ttattcagcc
     9481 cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt ttcgcgcgcg gcgttcgcca
     9541 gagcaagcgt gggcgtatcc agcgcgctga catccagatg tttaggccgt agctgggtat
     9601 ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg tgccatcggg tcaacaaatg
     9661 gcagcatgac caggcaacgt acaaggttgt agaagacgtg gaaatagatc accagttccg
     9721 cttttggcaa tgacagcttc cccattgtct ctgccagcaa atggacaaac ggcaggataa
     9781 tcaggctacc caccagctta aacagcagac tacccagcgc gacacggcgg gctgcggcat
     9841 tggcggcact gttgttgagc atcgccagca ggccggaacc gaggttagca ccaatcacca
     9901 gacagagcgc cacggggaag gagataatgc ctgcggcggt cagagtcgca gtcagcagta
     9961 cagcagcaag gctggagtag ctgataatgg cgaacatcgc gccaatcagc gcatccagca
    10021 gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc gtttgcctgg gtgatcggcg
    10081 ttacggcctg cacaatcaac tccagcgcca gtaaaatcag cccaagacca ataccgacgc
    10141 ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa aaaaatcacg ccgataaaaa
    10201 taagtaacgg tgacagccag gataagtcga aggtgagaat acgcgccatt agcgccgtcc
    10261 cgacatctgc ccccagcaca atgaccagag ccggtgcgag ggctaccaga tcctgagcga
    10321 caaacgaggt caccagcatg gtggtggcat tactgctctg caccagtgcg gtaacgccga
    10381 tccccgcgca aaaggcgagc ggcttttttt cgacgctccg gctaaggaca gtacgcaaac
    10441 gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg agtcccccag accagcaggg
    10501 cgacggcaga aagcaggtga agcagcgtta acacgagttc aggttctcct tatcgttata
    10561 tgttcctgaa gcaattgaat gaccaaagga ccggaaacac gctgactggc atctttactg
    10621 tgccttttga cattcgtttt tccggctaag attattccag cctgtttcca gtataagtgt
    10681 ttcatattaa taaagagaca gggcactcat tgagcgccct gtttgttgta aggaaaagtg
    10741 acagatttgt gaatcagtcc gcgtcatacc ccagattcgg tgccagccag cgctcaacat
    10801 cggagacgct cataccttta cggcgggcat agtcttcaac ctgatcgcgc tgaatttgcg
    10861 ccaccgcgta gtacttgctg tccgggtggc tgaaatacca acccgaaacc gatgcaccgg
    10921 gccacatggc gaaagattct gtgagtttca tgccggtatg tttttccact tccagcagct
    10981 cccagatggt ggctttttcc gtatgttccg ggcaggccgg gtagcccggt gccggacgga
    11041 tgccctggta gttttcgcgg atcagttctt cgttgctgag gttctcgttc ggcgcatagc
    11101 cccagtagac tttacgcaca cgctcatgga gatactccgc aaaggcttcg gctaaacggt
    11161 cggcaagcgc tttcaccatg attttgttgt aatcatcgtg ctgcgcttca aaggcatcag
    11221 ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc gccgatgtaa tctgctttac
    11281 cagaaagctt cggcgcaacg aagtcagcga gacagtagtt agcgaatccc gttttttcgg
    11341 tctgttgacg cagatggtgg ctgacgttga tcacatgggt gcgcgtttcg tcacggtaga
    11401 tttcaatgtc atcgcccaca cggtttgccg ggaacaggcc caccacgcca cgcggattca
    11461 gcgttttctc ggcgcttaat ttatccagca tgtcgttggc gtctttaaac agccgctgcg
    11521 cctcaacgcc caccacttca tcttccagaa tgcgcggata cttcccggcc agcgaccagg
    11581 tcataaagaa cggtgtccag tcgatgtaat tacgcagagt ttcgatgctg gcttcaactt
    11641 cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc ctgccagtca aaagcgaaat
    11701 cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg cggtttctta cgcccgtgct
    11761 gaatacgtac ggtttcgtac tccttgcggg tacgagcgac aaaatcatca cgctgagtat
    11821 cggaaagcag cgccgccacc acaccaacgg tgcgcgatgc gttctgtaca tacaccgtcg
    11881 ggccgctgta gttctgctca attttcaccg ccgtgtgcgc ttttgaggtc gtcgcgcctc
    11941 caatcaatag cgggatggtg aagccctgac gctccatctc tttcgccacg ttaaccatct
    12001 cgtccagcga cggcgtgata agccccgaaa gtccaatcag atcagcattc acttctttag
    12061 cggtacggag aattttttcc gcaggcacca taacgccgag atcgacaatt tcgtagttgt
    12121 tacattgcag caccacacca acgatatttt taccgatgtc gtggacgtcg cccttcacgg
    12181 tggcgatcac catcttgccg ttggttttgc cctgctcttt gctggcttca ataaacggtt
    12241 cgagatacgc caccgcctgt ttcatgacgc gcgccgattt gaccacctgt ggcaggaaca
    12301 ttttcccttc gccaaacaga tcgccgacca cgttcatgcc gtccatcaac gggccttcaa
    12361 tcacttcaat cgggcgcgta gcctgctggc gggcttcttc ggtatcctgc tcgataaact
    12421 cggtaatgcc tttgaccagc gagtattcca gacgtttatt cacttcccac gagcgccact
    12481 ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc gcgatatttc tcggcaagct
    12541 ccagtaaacg ctcagtgcca tcatcgcgac gattaagaat cacatcttcc accgcgtcgc
    12601 gcagttcagc gggcaggtcg tcgtaaatcg ccagctgccc ggcattgacg atccccatat
    12661 ccatgccatt gcgaatagcg tagtagagga acactgcgtg aatggcttcg cgcaccggat
    12721 cgttgccacg gaacgagaaa gaaacgttag atacgccgcc ggaaattagc gcgtgcggca
    12781 gttcgcgttt gatgtcttca cacgcgccga taaagtcctg cgcgtagttg ttgtgctctt
    12841 caatgccggt ggcaaccgcg aagatgttcg ggtcgaagat gatatcttct ggcgggaagc
    12901 caacctcttc ggtgaggatt ttgtacgccc gacggcaaat ctcgattttc cgtgcgcggg
    12961 tatcggcctg tccctgttcg tcaaaggcca ttaccaccac tgccgcaccg taacgacgca
    13021 agagcttcgc gtgatggata aaggcgtcga cgccctcttt catcgagata gagttaacaa
    13081 tgcctttgcc ctggatacac ttcagacctt tttcaatgac atcccatttt gaggagtcga
    13141 tcataatcgg cacgcgagcg atatccggtt caccggcaat cagattgaga aaacgcacca
    13201 tcgccgcttc ggcgtcgagc atcccttcat ccatgttgat atcgataatc tgcgcaccgt
    13261 tttccacctg ctggcgggcc acatccagtg cctcgctgta tttctcttct ttgatcaggc
    13321 gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc cacgttcaca aacaggctat
    13381 cttcgccaat gttcagcggc tccaggccgg acaaacggca ggctacgggg atttccggca
    13441 gtttgcgcgg cgctaatcct tctactgcac gactcatcgc tgcaatatgt tgtggcgtgg
    13501 tgccacagca gccgccgacg atattgagaa aacccgcttg cgcccattca cgtatctgtt
    13561 ttgccatcgt gtcggcgtcg agatcgtact caccaaaggc gttgggtagc ccggcgttcg
    13621 ggtgcgcggt gacgtagcat tccgcaatcc gtgacagctc ctgcacgtac tggcgcagtt
    13681 catcgggccc cagcgcacag ttcaggccaa aggtcagagc ttcggcgtgg cgcaatgagt
    13741 tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc ggaggcgtcg gtgatggtgc
    13801 cggagatcat aatcggcagc tcaacgccca gcgcttcaaa ctccgttttc accgcaaaaa
    13861 ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat caggatcaga tccgcaccac
    13921 cttccaccag cgctttggtg gattcgcgat aggccgccac cagctggtca aaagtgatgt
    13981 tgcggaatgc cggatcgttg acgtccggag aaatagacgc cgtgcggttg gttgggccga
    14041 gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc ggtccactcg tcagcacagg
    14101 cgcgcgccag tttcgccgcc gcaaagttga tttccgccga cagggattcc atctggtaat
    14161 ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc gatgatatcc gcgcccgctt
    14221 caaagtaggc gttgtggata gcggcgatca cttccggttt gctgagcacc agcaggtcgt
    14281 tgttgccttt gagatcgcat ggccagtcgg caaagcgttc accacgaaaa tcggcttcgt
    14341 tcagtcgata gctctggatc atggtgccca taccgccgtc cagcaccaga atacgttcat
    14401 ttaactgcgc acgcagttgt tccactttgc tgctcacact tgctcccgac acgctcaacc
    14461 cagatttaat aaaaattcaa caaaccatac tggcataaac gcatctgtgg taaaagcgac
    14521 caccacgcaa catgagattt gttcaacatt aactcatcgg atcagttcag caactattgc
    14581 attagctaac aataaaaatg aaaatgattt ccacgataca gaaaaaggag gctgtcatgg
    14641 tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt tgccaccgca ccagcgactg
    14701 gacaggttca gtctttaacg cgtggcctga aattactgga gtggattgcc gaatccaatg
    14761 gcagtgtggc actcacagag ctggcgcaac aagccgggtt acccaattcc acgacccacc
    14821 gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca ggtcggcgaa ctgggacatt
    14881 gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt tctccagagc cgtaatttgt
    14941 tagcgattgt tcaccctatc ctgcgcaatt taatggaaga gtctggcgaa acggtcaata
    15001 tggcggtgct tgatcaaagc gatcacgaag cgattattat cgaccaggta cagtgtacgc
    15061 atctgatgcg aatgtccgcg cctatcggcg gtaaattgcc gatgcacgct tccggtgcgg
    15121 gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac gaagctgctg caccgcaaag
    15181 ggttacatgc ctatacccac gcaacgctgg tgtctcctgt gcatttaaaa gaagatctcg
    15241 cccaaacgcg caaacggggt tattcatttg acgatgagga acatgcactg gggctacgtt
    15301 gccttgcagc gtgtattttc gatgagcacc gcgaaccgtt tgccgcaatt tcaatttccg
    15361 gaccgatttc acgtattacc gatgaccgcg tgaccgagtt tggcgcgatg gtgattaaag
    15421 cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg actttttctg gcgggcagag
    15481 gcaatattct gcccatcata cctgagtggc aatagaataa gggtgtctgt taatcgcatt
    15541 gacgccaaaa taacttaatg tcatacactt cacacacttt tattaactca gcattatttt
    15601 taaacatcaa actacttaat cattaacaaa atcgcaagat ctcattccac cgaaactttt
    15661 acggctgcat tatgagattg agcgatttat gatatttctt tagttgtaat catattaaat
    15721 tttttcactg ttctgatagt taaaattcta gacatcaata aacaatgaga tatttaaatg
    15781 attactcgta tccctcgtag ttctttctct gcaaatatta ataatacagc ccagacaaat
    15841 gaacaccaaa ccctgagtga attgttttat aaagaactcg aggataaatt ttctggcaag
    15901 gagctggcga cgcctctatt aaaaagcttc tcagagaact gtagacaaaa tggtcgtcat
    15961 atctttagca acaaggat
//
LOCUS       DAZGTR010000099        15819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGTR010000099 DAZGTR010000000
VERSION     DAZGTR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.100"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(156..2423)
                     /gene="adiA"
                     /locus_tag="ACHLCY_003539"
     CDS             complement(156..2423)
                     /gene="adiA"
                     /locus_tag="ACHLCY_003539"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP0370718.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2622..3530)
                     /gene="melR"
                     /locus_tag="ACHLCY_003540"
     CDS             complement(2622..3530)
                     /gene="melR"
                     /locus_tag="ACHLCY_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP0370719.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3813..5168
                     /gene="melA"
                     /locus_tag="ACHLCY_003541"
     CDS             3813..5168
                     /gene="melA"
                     /locus_tag="ACHLCY_003541"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP0370720.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5271..6692
                     /gene="melB"
                     /locus_tag="ACHLCY_003542"
     CDS             5271..6692
                     /gene="melB"
                     /locus_tag="ACHLCY_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP0370721.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6831..7460)
                     /gene="yjdF"
                     /locus_tag="ACHLCY_003543"
     CDS             complement(6831..7460)
                     /gene="yjdF"
                     /locus_tag="ACHLCY_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP0370722.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7583..9229)
                     /gene="fumB"
                     /locus_tag="ACHLCY_003544"
     CDS             complement(7583..9229)
                     /gene="fumB"
                     /locus_tag="ACHLCY_003544"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0370723.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9307..10647)
                     /gene="dcuB"
                     /locus_tag="ACHLCY_003545"
     CDS             complement(9307..10647)
                     /gene="dcuB"
                     /locus_tag="ACHLCY_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP0370724.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11218..11937)
                     /gene="dcuR"
                     /locus_tag="ACHLCY_003546"
     CDS             complement(11218..11937)
                     /gene="dcuR"
                     /locus_tag="ACHLCY_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP0370725.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11934..13565)
                     /gene="dcuS"
                     /locus_tag="ACHLCY_003547"
     CDS             complement(11934..13565)
                     /gene="dcuS"
                     /locus_tag="ACHLCY_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP0370726.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13746..13976
                     /gene="yjdI"
                     /locus_tag="ACHLCY_003548"
     CDS             13746..13976
                     /gene="yjdI"
                     /locus_tag="ACHLCY_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP0370727.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13988..14260
                     /gene="yjdJ"
                     /locus_tag="ACHLCY_003549"
     CDS             13988..14260
                     /gene="yjdJ"
                     /locus_tag="ACHLCY_003549"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0370728.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14487..14783
                     /gene="ghoS"
                     /locus_tag="ACHLCY_003550"
     CDS             14487..14783
                     /gene="ghoS"
                     /locus_tag="ACHLCY_003550"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0370729.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14811..14984
                     /gene="ghoT"
                     /locus_tag="ACHLCY_003551"
     CDS             14811..14984
                     /gene="ghoT"
                     /locus_tag="ACHLCY_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP0370730.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15103..>15819)
                     /locus_tag="ACHLCY_003552"
     CDS             complement(15103..>15819)
                     /locus_tag="ACHLCY_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HFP0370731.1"
                     /translation="SVRHNPEFTMMELYMAYADYHDLIELTESLFRTLAQEVLGTTKV
                     TYGEHVFDFGKPFEKLTMREAIKKYRPETDMADLDNFDAAKALAESIGITVEKSWGLG
                     RIVTEIFDEVAEAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGF
                     SELNDAEDQAERFQEQVNAKAAGDDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIML
                     FTNSHTIRDVILFPAMRPQK"
BASE COUNT         3846 a         3761 c         4058 g         4154 t
ORIGIN      
        1 aaataacagg caaaaaaaag cgcggggtgg ggccgcgcaa aaagtatgac atttttgttt
       61 gcagcaattt ttatgaaaac gttgccatac tcaaaaggcc taaggccagg cggcgtaatg
      121 ttatttaaac aattacgcct tcagcggaat agtggttacg ctttcacgca cataacgtgg
      181 taaataccgt caataatttc agtcccttca gtttcgtgtt caaatccagg gaaatggtgg
      241 tcccaggatt gcagcgagcg taaataactt acttgcggac tgtttttatc gccgaagttt
      301 tcaccagaca gcagcatcgg gattcctggc ggatacggga taactgagtt tgccgcgatg
      361 cgtcctggca gattttcaat ggataccagt tcgacattgt tgtcgacaat cgcgttgtac
      421 gcttcacgcg gggtgatttc cgccatcggc aggccggaat aggcttcgtt caaccgtgcg
      481 ccagggttgt tttctttcag ccaggcaaac atggtgtcac ccagatcgtg aatccccatg
      541 ttcgcgtaag tgtcaggata ttgttcaaca agttccggca tcacctgcgc cagcggtgtg
      601 ttggcgtcat agtggcgttt gaaggagcaa agggtgttaa ccagagtccc ccatttccca
      661 cgggttacgc ccatagagaa caggaacata atttggaagt cagtggtgcg ggtaggcaca
      721 atgccgtggc gaccaagcca ggcagtgacc agcgccgccg gaacaccggt ttcttccagt
      781 tcaccatctt cacccattcc cggagcaagg atgctgactt taattgggtc gagcatactc
      841 cagttatccg gaatatcttt gaagccgtgc cagctttcgc ccggatgcat tacccagcag
      901 tcctgaacgg tggtcagcag tttggttggt gcgtcagcaa agtcataggt tttgccggtt
      961 tgtgggtcgg tgacgacttc tttgttccac ggtttgaaga accagctacc gtcagcggtg
     1021 aactctttat atagccgcgc catcgcctga cggaaatcaa ccgcttcgtc aatcacttcc
     1081 tgtgtcagtg acaggccgct gttgccgtcc atcatcgaca ccgccacgtc gttggatgcg
     1141 cagatggcat acagcgggga ggtggtggca tgcatcatgt aggcctggtt gaagcgggag
     1201 aagttaatcg ccccacgacc ttcacgtaca tgaatataag aagcctgtga cagcgcattc
     1261 agcagtttgt gggtggagtg ggtggcgaaa acggtaggac cgttgtgatc gccaggttcg
     1321 ccgcgcatgg catagtgatc ggcatagatc gggttgaaac gtgcatagcc gtaccaggct
     1381 tcgtcaaagt gcagacgatc ggaggttttt tccagcagat cctgcgcttc tttagcgtta
     1441 taacacacgc cgtcataggt gcagttggtc accacgcagt aagacggttt ttgcccggct
     1501 ttgtctttgg tcagcgggct ttcactgatt ttcttctgca aggtttcagg ttgcatttcc
     1561 tgcggataga ttggcccgat aatgccgtag cggttgcggc ttggcaccat atagaccggt
     1621 ttcgcgcctg tcagcatcaa gccttgttcg atggatttat ggcaattacg gtcaacgacc
     1681 acgacatcgt tatcggtcat gcaagcctgc atgatggtgc ggttagagcc ggaagtaccg
     1741 acgactaccg accaggagcg atcggcacca aatacgcgtg cggcatattt ttcgctttcg
     1801 ccaaatgcgc cagtatggtc aagcaaagaa ccgagggaag ttcgttcgat gcccatgtcg
     1861 gtgcggaaca gattttcacc atagtagtca tggtagaaac gtccggcggg tgttttggta
     1921 aaaccaacgc cgccctggtg gcctggcgct gcccaggaat attcatggat gtcactatat
     1981 ttcatcagcg cgctgaacag tggcggcaac agctgctggc ggtagcgggt catcgcggca
     2041 acggcgcgtc cggcgataaa gtcggcggta tcttccagaa tccaggcgaa ttcatcgaca
     2101 agctccagca gatcccggtc cattgcggcg agggcttttt cccgatcgcc caacaggaag
     2161 accggcacgt tttgttggcg ctcatgaagc ttaccgatca attgtctgac gttttgatgt
     2221 tcgtccggat gttccatttg atagctgaac atcaggcagt caatggcttc gtttgaagag
     2281 agaatggcaa aaccatcatc aaaggaggtg gatttaatca cggtaacatt ttgctggctt
     2341 aaagcatctg ccagacgctc aacggcgtta ccgacccagg tgtcttgatg gagaaactcg
     2401 ctttcaacaa ttaatacttt catcattgct tacccggtta tgaaggaatc ttcgttgcgg
     2461 gcaagtatct tccggccttt tcgggctgta aagcgcgtga aaaacaaaca tttttgaaat
     2521 atttagtttt ataaacaata cgttatgtga ttattttaaa ttattttcac tgcttattat
     2581 cgacggctaa actatttttt tggctgatac tgatatcgtc tttagccggg aaacgtctgg
     2641 cggcgctgtt ggctaagttt gcggtattgt tgcggcgaca tgccgacata tttgccgaac
     2701 gtgctgtaaa aacgactact tgaacgaaag cctgccgtca gggcaatatc gagaatactt
     2761 ttatcggtat cgctcagtaa cgcgcgaacg tggttgatgc gcatcgcggt aatgtactgt
     2821 ttcatcgtca attgcatgac ccgctggaat atccccattg catagttggc gttaagtttg
     2881 acgtgctcag ccacatcgtt gatggtcagc gcctgatcat agttttcggc aataaagccc
     2941 agcatctggc taacataaaa ttgcgcatgg cgcgagacgc tgtttttgtg tgtgcgcgag
     3001 gttttattga ccagaatcgg ttcccagcca gagaggctaa atcgcttgag catcaggcca
     3061 atttcatcaa tggcgagctg gcgaatttgc tcgttcggac tgtttaattc ctgctgccag
     3121 cggcgcactt caaacgggct aagttgctgt gtggccagtg atttgatcac catgccgtga
     3181 gtgacgtggt taatcaggtc tttatccagc ggccaggaga gaaacagatg catcggcaga
     3241 ttaaaaatcg ccatgctctg acaggttccg gtatctgtta gttggtgcgg tgtacaggcc
     3301 cagaacagcg tgatatgacc ctgattgata ttcacttttt cattgttgat caggtattcc
     3361 acatcgccat cgaaaggcac attcacttcg acctgaccat gccagtggct ggtgggcatg
     3421 atatgcggtg cgcgaaactc aatctccatc cgctggtatt ccgaatacag cgacagcggg
     3481 ctgcgggtct gtttttcgtc gctgctgcac ataaacgtat ctgtattcat ggatggctct
     3541 ctttcctgga atatcagaat tatggcagga gtgagggagg atgactgcga gtgggagcac
     3601 ggttttcacc ctcttcccag agaggcgagg ggagcgtccg agcatcatga ggccgaaaac
     3661 tctgcttttc aggtaactta ttaccataaa ctcagattta ctgctgcttc acgcaggatc
     3721 tgagtttatg ggaatgctca acctggaagc cggaggtttt ctgcagattc gcctgccatg
     3781 atgaagttat tcaagcaagc caggagatct gcatgatgac tgcacccaaa attacattta
     3841 tcggcgctgg ttcgacgatt ttcgttaaaa atattcttgg tgatgtgttc catcgcgagg
     3901 cgctgaaaac ggcgcatatt gccctgatgg acattgatcc cacccgcctg gaagagtcgc
     3961 atattgtggt gcgtaagctt atggattcag caggggccag cggcaaaatc acctgccaca
     4021 cccaacagaa agaagcctta catgatgccg attttgtcgt ggtggcattt cagattggcg
     4081 gttatgaacc ttgcacggtg actgatttcg aggtctgtaa gcggcatggt ctggaacaaa
     4141 ccattgccga tacgttgggg ccgggcggta ttatgcgcgc gctacgtacc attccgcatc
     4201 tgtggcaaat ttgcgaggac atgacggaag tctgccccga tgccaccatg ctcaactatg
     4261 ttaacccaat ggcgatgaat acctgggcga tgtatgcccg ctatccgcat atcaaacagg
     4321 tcgggctgtg ccattcggtg cagggaacgg cggaagagct ggcgcgtgac ctcaatatcg
     4381 acccagctac gctgcgttac cgttgcgcag gtatcaacca tatggcgttt tacctggagc
     4441 tggagcgcaa aaccgccgac ggcagttatg tgaatctcta cccggaactg ctggcggctt
     4501 atgaagcagg gcaggcaccg aagccgaata ttcatggcaa tactcgctgc cagaatattg
     4561 tgcgctacga aatgttcaaa aagctgggct acttcgtcac ggaatcgtca gaacattttg
     4621 ctgagtacac accgtggttt attaagccag gtcgtgagga tttgattgag cgttataaag
     4681 taccgctgga tgagtacccg aaacgctgcg tcgagcagct ggcgaactgg cataaagagc
     4741 tggaggagta taaaaacgcc tcccggattg atattaaacc gtcacgggaa tatgccagca
     4801 caatcatgaa cgctatctgg actggcgagc cgagtgtgat ttacggcaac gtccgtaacg
     4861 atggtttgat tgataacctg ccacaaggat gttgcgtgga agtagcctgt ctggttgatg
     4921 ctaatggcat tcagccgacc aaagtcggta cgctaccttc gcatctggcc gccctgatgc
     4981 aaaccaacat caacgtacag acgctgctga ccgaagctat tcttacggaa aatcgcgacc
     5041 gtgtttacca cgccgcgatg atggacccgc atactgctgc cgtgctgggc attgatgaaa
     5101 tatatgctct tgttgacgac ctgattgccg cccacggcga ctggctgcca ggctggttgc
     5161 accgttaaaa cgcgactaaa cgctactgcg ccgggggatt tattccggcg cacacctctg
     5221 acgataccaa taacagaagg cgggcgttgg taacagcgac ccgataccct atgagcattt
     5281 caatgactac aaaactcagt tatggatttg gagcgttcgg gaaggatttt gcgatcggca
     5341 ttgtgtatat gtacctcatg tattactaca ccgatgtcgt tgggctgtct gtgggtttgg
     5401 tcggtacttt gtttctggtg gcgaggatct gggatgctat taacgatccg attatgggat
     5461 ggattgtaaa tgctacgcga tcgcgatggg gtaagttcaa accctggatc ctgatcggta
     5521 cgttggcaaa ctctgtaatc ttatttctcc tctttagtgc gcatctgttt gaaggtacta
     5581 ctcagattgt ctttgtttgc gtgacctaca tcctctgggg catgacttac accattatgg
     5641 atattccctt ctggtcgctg gttccaacca tcacgctcga taaacgtgag cgcgaacaac
     5701 tggttcctta tccgcgtttt tttgccagtc tggcaggctt tgttacggca ggtgtgacgc
     5761 taccatttgt taattatgtc ggcggtggcg atcggggatt tggctttcag atgttcactc
     5821 tggtactaat cgcctttttt attgtttcaa ccatcatcac tctgcgcaat gtgcatgaag
     5881 tcttttcgtc agacaatcaa ccgtctgctg aaggaagcca tctgacactt aaagccatcg
     5941 ttgcgctaat ttataaaaac gatcagcttt catgcctctt gggtatggct cttgcttata
     6001 atgtagccag caacattatt accggatttg ctatctatta tttctcatat gttatcggtg
     6061 atgcggattt gttcccctat tatctgtcgt atgcgggagc tgctaacctg gtgacgttag
     6121 tattcttccc acgcttagtt aaatcattat cccgacgcat tttatgggcc ggagcatcta
     6181 ttcttccggt gttaagctgt ggtgttctcc tgttaatggc attaatgagc tatcacaacg
     6241 tcgtcctcat tgtgattgcg ggtattttgc tgaatgtggg aacggcgctt ttctgggtat
     6301 tacaggtcat catggtggca gataccgttg attacggtga atataaactg cacgtacgct
     6361 gtgaaagtat cgcttactcc gtgcagacta tggtggtgaa gggcggttca gcctttgcgg
     6421 cttttttcat tgcggttgtg ttagggatga ttggctatgt accgaatgtt gaacagtcta
     6481 cgcaagccct attaggtatg cagtttatta tgattgctct accaactctg tttttcatgg
     6541 taacgctgat tctctacttc cgtttctatc gcctcaatgg cgacacgctg cgcaggatcc
     6601 agattcatct gctggataaa tatcgcaaag taccgcccga gcctgttcat gctgatattc
     6661 cggtcggtgc agtgagtgat gtgaaagcct gacgtggaca atagctaacg aaaatggcct
     6721 gatgcgatat gtttatcagg ccacgtctta atgtaatgct ttggatttgc aaattttgta
     6781 gaccggataa ggaattcacg ccgcatccgg catcaacaaa gcgcacgttg ttatccggtt
     6841 atcaagccaa agcgccgtag ctggcggcaa tgaaagcgag cgaggaatat caccgtcgtt
     6901 aatgcgccaa gcagcgcgca gaacatatcg gattgcgtgt cccactggtc gccctgggtg
     6961 ccgagaaaat catccgctcc ctggcccatt gccagcgccg cccaccactc gattaattca
     7021 tacatcgcgc ttatcgccag cgccacgcag cagaccagga acgccaccat tttacgtccg
     7081 cgcacgtaca tcccgcgcac gagaatttct cgtgccacca gtgcaggcac cagcccctgg
     7141 aaaaaatgcc ccagcttgtc atacggatta cggctcaacc ccagccattc ctgcacctca
     7201 aaaccgatgg ggactttcgc gtaggtgtat tgcccgccga ccatcaggat gatggcgtga
     7261 aagaaaatga gcgtatagag cagcggcgtt aacggataac gtttagcggt ggcaagcagt
     7321 agcggcacga caataatcac cggtgtcact tccatcagcc aggtgagttt gtcatgggcc
     7381 gaaatgccgg tataaatcag gattaacgtt agcgccagtg cgccggtgtt aagaattaac
     7441 ggcttgagtg tgcgggtcat ggtgagttcg caagtcaggg aaaatactga ctattcacca
     7501 gcaagcgtga aattacaatc gcaaaagagg tggcccaggg ggatcacctg gcagcatgct
     7561 gccaggcgct gggccgaaga ggttacttag tgcagttcgc gcactgtttg ttgacgattt
     7621 gctggaagaa gtcgttacct ttgtcatcga ccaggataaa cgccgggaaa tcttctactt
     7681 cgattttcca gatagcttcc atacccagct ccggataagc gacgcactcc agatgcttga
     7741 tgctctgctg cgccagtacc gccgccggac cgccgatgct accgaggtag aagccgccgt
     7801 gtttatgaca cgcgtcggta acctgctgac tgcggttacc tttcgccagc atgatcatgc
     7861 tgccgccgtg ggattgcagc agatccacgt aggagtccat acggcctgcg gtggttgggc
     7921 caagtgaacc tgatggataa ccggcagggg ttttcgccgg acccgcgtag tagatcgggt
     7981 gatctttgat gtactgcgga agttctttac cggcgtcaat cagctctttc agcttggcgt
     8041 gtgcaatatc tcggcccacg ataatggtgc cggtgagcga caaacgagtg gataccgggt
     8101 attgcgaaag ctgggcgagg atctctttca tcgggcggtt aaggtcaact ttcaccgctt
     8161 cgccttcacc ggcctggcgc agttcttgtg gaatgtactg gcctgggttg tgttccagtt
     8221 tttcgatcca gataccttcg cggttgattt tcgctttaat gttacggtca gcggagcagg
     8281 agacgcccat gccgaccggg caggatgcgc cgtgacgtgg cagacggata acgcgaatgt
     8341 cgtgcgcgaa gtatttaccg ccaaactgcg cgccaagacc gagtttctgg gcctcttcca
     8401 gcagttcctg ttccagctgg acatcgcgga acgcctgacc atgttcgttc ccttccgtcg
     8461 gcagttcatc gtaatagtga gcgcttgcta acttgacggt tttcaggttg gtttccgcag
     8521 acgtaccgcc aatcacaaac gcgatatggt acggcgggca ggctgcagta ccgagggtac
     8581 gcattttctc gacgaggaag tttttcagtt tgccgggagt cagcagggct ttggtttcct
     8641 ggtagagata cgttttgttg gcagagccgc cgcctttcgc aacgcaaagg aatttgtact
     8701 catcgccatc taccgcgtac aggtcgattt gcgcaggcag gttagtgccg gtgttgacct
     8761 ctttgtacat gtccagcgcc gcattctgtg aatagcgcag gttatcttcg atataggtgt
     8821 tatagacgcc tttcgacagc gcttcttcat caccgccacc ggtccacacg cgctggcctt
     8881 ttttaccgac gatgatcgcg gtgccggtat cctggcaggt cggcagcacg cctttggcgg
     8941 cgatttcgga gtttcttaag aattgcagcg ccacgtactt gtcgttttcg ctggcttctg
     9001 gatcgtgaag aatagccgca acctgtttct ggtgtgccgg acggagcatg aaagaagcgt
     9061 cgtgaaaggc ttgctgcgcc agtagggtca gggcttctgg ttccactttc aggatggttt
     9121 cgccgtcgaa gtcggcaacg ctaacgtaat cggaagtgag tagatagtat tcggtattgt
     9181 ctttccccat cgggaaaggt gcctggtaga taaagggttt gtttgacata gcttccagcc
     9241 tgtaactctg tatttgttat tcgaaaatgg cgtgccgcgt agtcacgggc ggcacgcaaa
     9301 gtgcatttat aagaacccgt acatcgcggc gaagatccag ccgaagacgc acgatacgct
     9361 cacaccaatc aaccccggca gaataaaact gtggttgatg acgaagcgac cgatgtgggt
     9421 ggtgccggaa cggtcaaact gaatcgctgc cagatcgctc ggataagtcg gcaggatgta
     9481 ataaccgtag caagccggtg ctgaagccac gatgtatgcc ggatcaacgc cgatcgccag
     9541 cgcgaccgga acaatcgccg ccagcgccgc agcctgagag tttacaaact tggaaaccag
     9601 cagcagaaca atggcatacg cccacggata ctctttcacc atttcaccca gtacgccctg
     9661 aatttcagac atatgcgcac cgaacatggt ttctgccatc catgcgatac cgtacaccgc
     9721 cacgatggcg atcataccgg aacggaagac ttcgtttttt gagatagacg cgggattggt
     9781 tttggtcagg ataataatca gcgccccggt cagcagcata aacatctgaa taaccagtac
     9841 catcgacagc ggtttgccgc cgaaggatgg acgcaggtcc gaatcagcac caagaagggc
     9901 gactacagcg attgcaccga ggaaaatcca cattgccagc cagttgcttt tcggcagttt
     9961 tttatcgagc agcgtcgcgg tatcaccgta aacatactca cggttttccg gtacggagat
    10021 gaatttctgg aactcttcgt ctttatccag atctttaccg cggaaccagc tgaagatacc
    10081 gatcgccagg ataccgatta acgtcgatgg aatggtgatt gccagcaggt cgaggaactc
    10141 aagatggcga ccatcaaagg tgacattacc cagcattgca accagagaca cgaccgcaac
    10201 cgacaccgga ctggcgataa tccccatctg tgcaccgata gaacttgccg ccatcggacg
    10261 ttccggacgg atgttgttct taatggcgac gtcgtagatg atcggcagaa tggtgtaaac
    10321 cacatgaccc gtaccgcaaa gaatggtcag tgtacaggtc acaaacggcg cgacaattga
    10381 gacatatttc gggttgcggc gcagcagctt ctcggcaatt tgcagcatga catcaagacc
    10441 gcccgaagct tgcaaggtcg ccgatgccgc caccaccgca atgataacca gcatgacatc
    10501 aaccggtggt ttacctggct gaaggtggaa gacgaagacc agaatgacca gaccgatacc
    10561 gcctaataaa cccagcgcga taccaccctt tctggcacca taaaacagac atatcagtat
    10621 tatgataagt tggatagtaa ataacatgtg tgaaccctcg cgataatcct atttaaattt
    10681 ttgctgaata gatcacagtc acgttctgtt ttgtatgaac tgttttcaga gcctgactaa
    10741 atatccgttt ggtcgttacc ggctttagca aatacctcac agtaaatatt ggctgattaa
    10801 agcagcggca aatttatgca ctgtgataag cggctttttc agagagaaaa actctctggc
    10861 gatgggttta tagtttgtac tgcgcagatg ttaatgtttt gtttgctgtt atcttattga
    10921 tatttatcgg tctatttttt gttctcaggg cagatatcat taggtttaaa gattaatctg
    10981 atctacccat ttgtgggtaa aaatacacat aatgcgggtg acataatagt taattaactt
    11041 ttgttatcgt tttgaaatta aaaacaccgt tcacctgaag agatattaat ttttagcgat
    11101 gatggaggga taattatatt tgatctggca caagttttac tgatgaagga tgtaacttgt
    11161 gccaggggga ttttgcatta cggtaataat tattacttac agataaccag cgatccgtta
    11221 ttggcaatat tgtttcagta gtgagtagtg ttctgcctga atacggtaac ggtaaaccgg
    11281 acgccccgtg acgccataat ggatactggt gaacaagatg tggcagttga ccagccagat
    11341 gaggtattta cggcaggaaa cacgcgagat gttaacctcg ttagccagct cgtcggttga
    11401 aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaaag tctgcggcgt
    11461 taagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt ggattagctg
    11521 atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct tttgccgcca
    11581 gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt aatccacgac
    11641 accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga tgacaatcac
    11701 atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt tctctttttg
    11761 catatagata tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga taatctcttt
    11821 ggctttctcc agcgtcgagg ctgttccaca gcattgaaag cctgggattt gtgctacgta
    11881 tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat tgatcatctg
    11941 ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat cccgggttcc
    12001 gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag tgctaaaccg
    12061 acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc gattttgtcg
    12121 ggtgcgattc ccggtccatc atcgttaact tcgcagtgca gccagccgtg acggtagtgc
    12181 aatgttacgc taatttcgcc accgggttcc ggccctaatg cttccagcgc gttttctatc
    12241 agatttccca atgtggtaat cagcgtcgcg acctggtcct cactgccgct gtctggcagc
    12301 tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt aatcttgctg
    12361 attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc aatctcttcc
    12421 tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact cttcagatgc
    12481 aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc acgaagtgca
    12541 tcagcatagt tgaccagacc gtcgagtcgc tgcatcagtt tacgtacttc agttttgtcc
    12601 ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac ggtgttgatc
    12661 agtaatagcc ggtctttaat cgtaatctct tcgtcgcggc gcggcgtacc gtcgcgtaac
    12721 acttccgaga catctaccac ctgtgaccat gagtggctga gcgtcgacag tttctcatcg
    12781 tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag cgtgacctcg
    12841 ccgcgatcgt ccacggcaac gacgccttct ttgatagact gcaacatggc ctggcgctgc
    12901 tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt cagtacctta
    12961 accagaatgc aggtgccaat cagtccgacc agcatgccaa ataataccga ccagataatg
    13021 ctccagcgac tgtcattgat ttgttgggtc acgcggctta actcaaggcc gatcgccacc
    13081 acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa agcctgcgcc
    13141 agaaaaccgc gattgatagc gacattttct ttgccattca gcgctttaag gatgtcatca
    13201 cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag actttgcata
    13261 tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc gatggcctgg
    13321 attccgctct cctgcggttt tttctgcaag ccctgacgga tttccggcga gtcggcgagg
    13381 gtacgcgcca ctgccagtgc cttgttggcc agcccgtctc gcgtcatatc actgatttgc
    13441 gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcgct gaccattaag
    13501 atcactgtgg tactcaattt catcggacgt ttgcgtaaca tgcggtaggg caatgaatgt
    13561 ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag gcgggtaatt
    13621 caaagggagt aagaatgatt ggctatatag gggaagagac tctggcaacg gaaactgcca
    13681 gtgctgtatg aagattccag ggctatgctt atagagataa tcatactgat gagagaggga
    13741 aagtcatgga tcaggcgcta ctggacgagg gttatcgctg ttataccggc gaaaagatcg
    13801 atgtctattt caacactgcg atatgtcagc attctggcaa ttgcgtacgt ggcaacggca
    13861 agttatttaa tctcaaacga aagccgtgga tcatgccgga tgaagtcgac gtcgtcactg
    13921 tggttaaagt gattgatacg tgcccgagcg gcgcgctgaa ataccgtcat aaataagcga
    13981 gggtaaaatg gaaatacgcg aaggccacaa taaattttac attaatgacg aacaaggcaa
    14041 gcaaatcgct gaaattgtct ttgtgccgac cggagagaat ttagcgatta tcgaacatac
    14101 cgatgtcgat gaaagcctga aagggcaggg gattggtaaa cagctggttg cgaaagtcgt
    14161 ggaaaaaatg cgtcgggaaa aacgaaaaat tatcccacta tgcccatttg cgaaacacga
    14221 atttgataaa acgcgggagt atgatgatat tcgcagttga tgggagagta cagagtcacg
    14281 atatttttca tgctctccgc ggagtgatgc aggagagcat ctgaagggta gggggatgca
    14341 caaagaatgg gcagagagcg cgtttttttg tcccaagtca tcccctttac tgagcaaaaa
    14401 aaagaatatc tcctatatga gaatcatcaa tcggggttaa taatttttgc gtccccagag
    14461 cattcaatat tgataggagt catattatgg aaggtaaaaa caagttcaat acttatgttg
    14521 tttcttttga ttatccatca tcttattcct cagtgttctt aaggttaaga tcattgatgt
    14581 atgatatgaa tttctcttct atcgtggctg atgaatatgg gataccacga caattgaatg
    14641 aaaattcctt cgcaataacg acatcgttag ccgcaagtga aatcgaagat ttaatcaggc
    14701 ttaaatgctt agacttaccg gatattgatt ttgacctcaa cattatgaca gttgatgact
    14761 atttccgtca gttttacaag tagccaggaa ggataagaag aacataaaaa atggcactat
    14821 tctctaaaat attaattttt tatgtgattg gtgtgaacat atcctttgtc attatctggt
    14881 ttatctcaca tgagaaaaca catattcgtt tacttagtgc attcctggtc ggaataacct
    14941 ggccaatgag tctgcctgtg gcattacttt tttctctctt ttaggagctg tagttgagtg
    15001 gacccgctgg ttatcgtgaa acgaagcgtc tctcctcaaa actggatttg cggaacgacg
    15061 aacgaagcag tcgcctgact gcttcgttca ttaaagtgaa atttatttct gtgggcgcat
    15121 cgccgggaag agaataacgt cgcgaatagt atgactgttt gtaaacagca taatcattcg
    15181 gtcgatacca atacccagac cagcggttgg cggcagacca tattccagcg cagtcacgta
    15241 atcttcgtca tagaacatgg cttcgtcgtc acctgcagct ttagcgttaa cctgttcctg
    15301 gaaacgttca gcctgatctt ctgcgtcgtt taattcgcta aaaccattac cgatttcacg
    15361 accaccgatg aagaattcaa aacggtcggt gatttctggg ttaacatcat tacggcgtgc
    15421 cagcggtgac acttctgccg gatattccgt aataaaggtt ggctgaatca gatgtgcttc
    15481 tgccacttca tcaaagatct ccgtgacaat acgtcccagc ccccagcttt tctctaccgt
    15541 aataccgata gattcagcta atgctttagc agcatcaaaa ttatccaggt cggccatatc
    15601 ggtttctgga cgatattttt tgattgcttc gcgcatggtg agtttttcaa acggtttgcc
    15661 gaaatcaaac acatgctcgc cataagtgac tttagtggta cccagaacct cttgtgccag
    15721 ggtgcggaac agggactctg tcagttcaat caaatcgtgg taatccgcat acgccatgta
    15781 gagttccatc attgtgaact caggattatg gcgaacaga
//
LOCUS       DAZGTR010000100        15506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGTR010000100 DAZGTR010000000
VERSION     DAZGTR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.101"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..564
                     /gene="yceJ"
                     /locus_tag="ACHLCY_003553"
     CDS             <1..564
                     /gene="yceJ"
                     /locus_tag="ACHLCY_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0370732.1"
                     /translation="SFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGWY
                     HKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLFA
                     IGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHGF
                     MALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            568..1143
                     /gene="yceI"
                     /locus_tag="ACHLCY_003554"
     CDS             568..1143
                     /gene="yceI"
                     /locus_tag="ACHLCY_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP0370733.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1185..2237)
                     /gene="trhO"
                     /locus_tag="ACHLCY_003555"
     CDS             complement(1185..2237)
                     /gene="trhO"
                     /locus_tag="ACHLCY_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP0370734.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2462..3382
                     /gene="lpxL"
                     /locus_tag="ACHLCY_003556"
     CDS             2462..3382
                     /gene="lpxL"
                     /locus_tag="ACHLCY_003556"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP0370735.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3554..4780
                     /gene="mdtG"
                     /locus_tag="ACHLCY_003557"
     CDS             3554..4780
                     /gene="mdtG"
                     /locus_tag="ACHLCY_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP0370736.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLTDRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4863..5237
                     /gene="msyB"
                     /locus_tag="ACHLCY_003558"
     CDS             4863..5237
                     /gene="msyB"
                     /locus_tag="ACHLCY_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFP0370737.1"
                     /translation="MTMYATLEEAIDAAREEFLADTPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5238..5465)
                     /gene="yceK"
                     /locus_tag="ACHLCY_003559"
     CDS             complement(5238..5465)
                     /gene="yceK"
                     /locus_tag="ACHLCY_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0370738.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5638..8181)
                     /gene="mdoH"
                     /locus_tag="ACHLCY_003560"
     CDS             complement(5638..8181)
                     /gene="mdoH"
                     /locus_tag="ACHLCY_003560"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP0370739.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8174..9709)
                     /gene="mdoG"
                     /locus_tag="ACHLCY_003561"
     CDS             complement(8174..9709)
                     /gene="mdoG"
                     /locus_tag="ACHLCY_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP0370740.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10103..11260
                     /gene="mdoC"
                     /locus_tag="ACHLCY_003562"
     CDS             10103..11260
                     /gene="mdoC"
                     /locus_tag="ACHLCY_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP0370741.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11268..12749)
                     /gene="clsC"
                     /locus_tag="ACHLCY_003563"
     CDS             complement(11268..12749)
                     /gene="clsC"
                     /locus_tag="ACHLCY_003563"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP0370742.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12691..13224)
                     /gene="ymdB"
                     /locus_tag="ACHLCY_003564"
     CDS             complement(12691..13224)
                     /gene="ymdB"
                     /locus_tag="ACHLCY_003564"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP0370743.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13319..13630)
                     /gene="ymdA"
                     /locus_tag="ACHLCY_003565"
     CDS             complement(13319..13630)
                     /gene="ymdA"
                     /locus_tag="ACHLCY_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP0370744.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(13751..14083)
                     /gene="csgC"
                     /locus_tag="ACHLCY_003566"
     CDS             complement(13751..14083)
                     /gene="csgC"
                     /locus_tag="ACHLCY_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP0370745.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14142..14597)
                     /gene="csgA"
                     /locus_tag="ACHLCY_003567"
     CDS             complement(14142..14597)
                     /gene="csgA"
                     /locus_tag="ACHLCY_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP0370746.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14638..15093)
                     /gene="csgB"
                     /locus_tag="ACHLCY_003568"
     CDS             complement(14638..15093)
                     /gene="csgB"
                     /locus_tag="ACHLCY_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP0370747.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3862 a         3982 c         3689 g         3973 t
ORIGIN      
        1 tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca ttggctaagc
       61 gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct cagttattac
      121 gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt gttaatgatg
      181 gggctggtta ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc gctgccgagt
      241 tattcgccca tgactcgtct tgccgcaaag gctggacatc tcgcattgta cctattgctg
      301 ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc gatcagcgtt
      361 tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc agactttgcc
      421 ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtccgttat gcacggattt
      481 atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat gctgggaaag
      541 tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt aaccttcgcg
      601 tccctgatgt tctctgccgg ttcagcggtt gccgccgatt acaaaattga caaagaaggt
      661 cagcacgcct ttgttaactt ccgcatccag catcttggct atagctggtt atatggcacc
      721 tttaaagatt tcgacggtac ttttaccttt gacgaaaaaa atccggctgc cgataaagtg
      781 aatgtgacaa ttaacaccac cagcgtcgat actaatcacg ccgaacgcga taaacatctt
      841 cgcagtgcag atttcctcaa taccgcaaaa tatccacagg caacattcac ctccaccagc
      901 gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa tggcgtaacc
      961 aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg gggtggtaaa
     1021 cgtgcaggct tcgaggccga aggcaaaatt aagctcaagg acttcaatat caagacagat
     1081 ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt acagcagaag
     1141 taagcgtgaa atgttgccgg atggcacgca tccggcaatg atatttattc cgttggatca
     1201 ggaatgccca gtgttgtatt cagacgtcca cgagacttat taaagatctt attgccattt
     1261 tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc ttcgcagcaa
     1321 atctcactac aacaaccttt gtatttttcc gcacatactg gacactgaat aaacagcagg
     1381 tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca ctggtggcaa
     1441 tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac aaaatttttg
     1501 ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc aataattccg
     1561 ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact ggccttttca
     1621 caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc ctgcatcatc
     1681 tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag cgcgttttca
     1741 aagtgcccca cttcatactc atagtggtta cgcatgtcga taaatagtgc atcgggatcg
     1801 tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct ggcatcaaag
     1861 tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg cagtacccag
     1921 aaagatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa agccgggtcg
     1981 aaggcataga gctgcgcgcg aaatgtttca acattgctcg caggtacgct gatttgcgcg
     2041 ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt aaacagctga
     2101 tataaagcgt cacgggtcgc cttaggatcg gcgatgtgga aatacttgta aaacgaaatg
     2161 gtggttcgcg gttcgctctc agccaacatt ttggctttta gcgcgtcgtt ggaaatgcgg
     2221 ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa atccgcggca
     2281 tgatatagca attatcgata attaacatcc acacatttta cgctacattt gcgcattaaa
     2341 aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagccaca aattttggtt
     2401 gcgggcgaaa aaatgcgaca atacatacaa ttgcccgaat aggttgaaaa acaggattga
     2461 tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt ggttaacctg
     2521 gttgggtatt ggcgtacttt ggttagtcgt gcaattgccc tacccggtta tctaccgcct
     2581 cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa aaattgtgca
     2641 tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta aaatggtggt
     2701 gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggcatggcgt ggttctggcc
     2761 ggaccgccga atcgcccgct ggacggaagt gatcggcatg gaacacattc gtgacgtgca
     2821 ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg agctgggtgc
     2881 gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg ataatccact
     2941 gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc tcgaccgcaa
     3001 agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt acgcaccgga
     3061 tcatgattac ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg agcaggctgc
     3121 gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg tgcccttcgt
     3181 tccacgccgt aagccagatg gcaaagggta tcaattgatt atgctgccgc cagagtgttc
     3241 tccgccactg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg tcgaaaaatg
     3301 catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa cacgcccgga
     3361 aggcgttcct tcacgctatt aaatctcccc tgccggatgc ttcagaatgg catccggcat
     3421 taccacagca aatccccctg atttagcgat aaaagctctc tggattgcgc cccctggaag
     3481 tcgggcgcat aattagtgtg cttatctttt cttcttatgt tcaccgcgcc tggcgcacca
     3541 acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga aacgaaacct
     3601 gatcgtcgcc tggctaggct gttttcttac cggtgccgcc ttcagtctgg taatgccctt
     3661 cttacccctc tacgttgagc agcttggcgt taccggtcac tccgccctga atatgtggtc
     3721 cggtattgtc ttcagcatta catttttatt ttcggccatc gcctcaccgt tttggggtgg
     3781 actcaccgac cgtaaaggcc gaaaactcat gctattacgc tctgccctcg gcatgggcat
     3841 cgtgatggtg ttgatgggac tggcacaaaa tatctggcag tttttgatcc tacgggcgct
     3901 tcttgggtta cttggcggat ttgtccccaa cgctaatgct cttatagcca cacaagtacc
     3961 gcgtaataaa agcggctggg cgctgggtac gctctccaca ggcggcgtta gtggtgcgtt
     4021 gctcggccca atggctggcg gcctgctcgc cgatagctac ggcttacgtc cggtattctt
     4081 tattaccgcc agtgtgctca tactctgctt tttcgtcacc ctgttctgca tcagagaaaa
     4141 attccagccg gtcagcaaaa aagagatgct gcacatgcgg gaagtggtga catcacttaa
     4201 aaacccgaaa ctggtactca gcctgtttgt caccacgtta atcatccagg tggcgacagg
     4261 ctcaattgcc cccattctga cgctgtatgt ccgcgaactg gcgggtaacg tcagtaacgt
     4321 cgcctttatc agtggcatga tcgcctcggt gccaggcgtg gcggctctgc taagcgcacc
     4381 acgactcggc aaacttggcg atcgaatcgg acccgaaaag atcctgatta cagcgctgat
     4441 cttttctgta ctgctgttga tcccaatgtc ttacgttcag acgccattgc aacttgggat
     4501 tttacgtttt ttgctcggtg ccgccgatgg tgcactactc cccgccgtac agacactgtt
     4561 ggtttacaac tcgagcaacc agatcgccgg gcgtatcttc agctataacc aatcgtttcg
     4621 tgatattggc aacgttaccg gaccattgat gggagcagcg atttcagcga actacggttt
     4681 cagagcggta tttctcgtca ccgctggcgt agtgttattc aacgcagtct attcatggaa
     4741 cagtctacgt cgtcgtcgaa taccccaggt ttcgaactga tttttcgcct ttcatacttg
     4801 caaaagcgga gaatcagcta tccttttccc tgaaacctca tcaactcaaa gggagaatcg
     4861 tgatgaccat gtacgcaacg cttgaagaag ccattgacgc tgcacgcgaa gaatttcttg
     4921 cagacacccc cggcatcgac gccgaagatg cgaatgtgca acagttcaat gcccaaaaat
     4981 acgttttgca ggacggcgac atcatgtggc aagttgagtt ttttgccgac gaaggggaag
     5041 aaggtgaatg tttacctatg cttagcggtg aagccgcgca aagtgttttt gatggcgact
     5101 atgatgagat agagatacgc caggagtggc aggaagagaa tacattacat gaatgggacg
     5161 agggggaatt tcagcttgag ccaccgctgg ataccgagga aggacgcgca gcagctgatg
     5221 agtgggatga acgttaatca ctcatacggg ccatgatgaa tgtcgatcgg cagcagtaaa
     5281 gtatcaaaaa ccagagagaa tggcagatca aggatcgtga cataacgcca ggcggagtca
     5341 cgcacatccc attgcacacc gggataatat tggttgccat gcccctgccc cggtatagtg
     5401 cgactaataa tgctgccaca gccactcagc aaggtcacca tgatgctcac cacaattaaa
     5461 cgcatttgat acctctttcg taaaaaaata ccggcataac gccggtattt ttattggctc
     5521 gttgtttttg ccggatgcgg cgtgaacgcc ttatccgacc cacaaaatct tgcaaaatca
     5581 ataaattgca ggaacgatgt aggcctgata agcgtagcgc atcaggcaac tacgttttta
     5641 ttgcgaagcc gcatccggtt tacgcaatgc cagtggattg agctttatcc cttcgtaata
     5701 actcacccat gaagaatatc tctccgggga attccagacg cggaaatgca gacgggccat
     5761 cgtcaccgga tcgcttagca gcaccaggcg acgatcgcga ttcagcttct ctggcgtctc
     5821 gttcagcgcc tgttcaacgt ggcggtcacg ggcgatttcc agcaccttac tggcgcggtg
     5881 acgcgcggtc gccattgcgg ttgccagagc gttaaatgac gggttaaaca ctgcgtgcat
     5941 aaagccatca tcaagggagc gttgacgatt catctcaagg aaccgatcgg tatcaaccag
     6001 cacctgcggc ggcgaatact cttccgggat caggaacagt ttccagcgtt tggtgcgcag
     6061 accaacggtg gcacggctgg aaatcaccga aacaaacggt gacaggatca acgagaagac
     6121 aatcggtgcc agccagaaca ggaaacgcag atccaaccac gccataccaa cagcccacac
     6181 taaccctaac agcagctgtg agccgtggcg tttgaacgct tcaccccagg aagtggagtc
     6241 atcatcacgc tgcggtgaat tccacaccac ttcccagcca aggaacgcgc tgacgacgaa
     6301 gaccgtatgg aacagcatgc gtaccggagc cagcagcacg gaaaacagca cttccagcag
     6361 caacgataat gtaacgcgcc agaagccgcc gtattctttc gttcctttgc accagataag
     6421 caaaatgctc aataacttcg gcaggaacaa cagcaccatg gtcgaagcaa aaagtgcaat
     6481 cgccagctca ggacgccact gcggccacac cgggaacaac tgccgtggtt gcaggaagta
     6541 ttgcggttcg gtcaacgcat gtactacctg caatgcagta gagagcgcga ggaacataaa
     6601 ccacagcgga gcggagagat aagacatcac gcccgtcagg aataccgcac ggtgaaccgg
     6661 gtgcataccc ttcaccagga acagacggaa gttcatcagg ttaccgtggc accagcggcg
     6721 gtcacgtttt agctcatcaa gcaagttagg cggcaattct tcataagaac ccgggagatc
     6781 gtaagcaatc cagacccccc aacctgcacg gcgcatcaac gccgcttcca cgaagtcatg
     6841 tgacaggatt gaaccggcaa aggaaccttc gcccggcagc ggagccagtg cgcagtgctc
     6901 gataaacggt ttcacgcgga taatcgcgtt atgtccccag tagtgcgact cgccaagttg
     6961 ccagaagtgc aaaccggctg taaacagtgg cccatacacg cgggtcgcga actgctgaca
     7021 gcgcgcatac agcgtatcca taccggacgc tttcggcgac gactgaatga tcccggcgtt
     7081 cgggttggct tccatcaggc gcaccaggcc gcataaacaa tcaccggtca ttaccgagtc
     7141 agcatccagc accaccatgt agctgtactg gctgccccag cgacggcaga agtcatcgat
     7201 attaccgctt ttacgcttca cgcggcgacg gcggcggcga tagaaaatct gaccttcgcc
     7261 accgacttca gcgataagct ccatccaggc tttttgctct gcgacgcaga tatccgggtt
     7321 ataactgtca ctaagaatgt agacatcaaa gtgtttggca ttcccggtgg cttttactga
     7381 ttcccacgtt gcacgcaggc cagcaaaaac acggttcacg tcttcgttac agataggcat
     7441 gatcaacgcc gtgcgatgct ccgggtttaa tggttcatcg ccaactgttg acgcagatat
     7501 actgtattta tcgcgaccaa taagcagttg caggaagccc attaacgccg tccagaatcc
     7561 ggcggacacc caacagaaca gtaccgcaaa gaggatcagg ataccggttt gcagcatata
     7621 aggcagaagc tgcataaagg aaacccacaa atcctgacca accatatcca taggattaat
     7681 cagcgcccaa ccctgataag gaagaatggt cttcatatac caggtcgcga cgacagtttg
     7741 cgcgagagtc aggatcaaca gaatgtaacg gcggatggta ccgacggtac gccacttttg
     7801 ctcgctctcc tgctcttctt tggtcaaacg agccagatag cgcggcgtga catcgcgtcc
     7861 acgcaggcga tcccagaaac ggcctaccgg gttggtacgc cacgggtcgg gaaacatcga
     7921 ggagcgtttt gcttctggca tcgccttcag ctgatcgcgc ccttcgtcgt ctttaattaa
     7981 ctgtccatca gcaagtgaat ctggccaggc ttgttccaga cgcgccttta ccgagccttg
     8041 cggggaatca tcctcccgcg cccaggtgcg gtgttcggca tccagcgcct gatgaacggc
     8101 gcggatatca gtcttcggca atgccgcttt ctcgcttgcg gcgatgggca ttgcgtcaat
     8161 gtactcagtt gtcttattca ttggcaggta actggtagct ccaggtttca ctcaacgtct
     8221 gatcggcatt caccagcgca gcacgcattt cagtggtttt cttggcatct ttcactttca
     8281 cacgcatcac cagacgccag cctttggtaa ccgggttata gcgcaccgtg ctttcaacta
     8341 tctcaccatt atcaccaatg ctggtttgcg ctgtgaccgg ggtatcctct ggcagttttt
     8401 tcatctctgc gccggtaaaa tcgaccacaa aggcgatagt accgtcaggc tggcgaatca
     8461 ggttcgactg cttcacatcc cccgttgaac gacgcgtttg ttgcacccat gcgttatctg
     8521 gcgcatgcag tttgtcttca tcacggctga aggtgatggt gtatttaaag ttcatctctt
     8581 tacccggctc cggcagctga tccggcgtcc agtaagcgac gatgttatcg ttggtttcat
     8641 cgttggttgg aatttccacc agctcaacgc tgcctttacc ccactcccct ttcggagtca
     8701 cccatgcgct tggacgaaga tcgtaacgat catcgagatc ttcaaagcgg gagaaatcac
     8761 gaccgcgctg caacagaccg aagccttgcg ggttctccat cgagaagctg ctgaccgcta
     8821 aatgtttcgg gttattcaac ggacgccaga tccattcgcc attaccggca tggatagaca
     8881 gaccgttaga gtcgtgcaac tccggacgat agttatttgc aggcgacggt tggttcggcc
     8941 caaacaggaa catactggtt aacggtgcaa cccccagttt gccgacttta tcgcgcagat
     9001 agattttcga ctgcacatcc acaaccgtgt cacgccctgg cataaccacg aatttgtaag
     9061 cacctgtcgc gcgcggcgag tcaagcaatg cataaatggt taaacgttta tcagtcggtt
     9121 ttggacgctc gatccagaac tctttgaagc gtggaaattc ttcacccgat ggcaaggcgg
     9181 tatcaattgc caggccgcgg gcagaaaggc cataaacctg acctgcacca atcacgcgga
     9241 aatagctggc cccgagcatg ctgacgattt catcgttttt atctttgctg ttgatcgggt
     9301 aaagcacttt aaaaccggca aaaccaaggt cttttaccgt gtctttgtca tgctgaacat
     9361 cgccgaaagt gaaataatcc gggctgtatt tgattcgttt gactgcggtg gcagtcactt
     9421 catttatttt gaccggggta tcgaagtaca taccctgatg gtagaactcg agtttgaatg
     9481 gggtcttcag attgttccag tacgctttgt catgattaaa ctggatctgc tgatagtccg
     9541 cgtatttcat atcgcggaaa acggagggca agttgctttt gggcgcctca tagcctttcc
     9601 cggctaagga ttgagcttgc tttgcgacat catcaatact gaaagcccag cttgaagatg
     9661 tatacagggt taacattact gcagcactca accaacgcat tttcatcatt tgtagtttat
     9721 gtttcataat tagtaagcac ttcccccttt gtgtgcttat atcgatccga tctattttaa
     9781 tggataacca gccattccga caacagaatc ccggtatcgt tcagcacgct ggctcatgga
     9841 ttaagcacct gattgcaacc gttttcactt tccgtgcttc tcctaaagac agaagcatcg
     9901 acttcgtgta gggtttgcca gcaattttat ttatcaaccg tcttacagac gcttccattc
     9961 tggttagtgg tagcgatgat gctatgcata aaaaccttga agcctgcgcc tggcgcggtc
    10021 aggtcggtgt cgctatggca acaagcctaa aggcataacc cgacagggat aagacgaaaa
    10081 atccgagatt agttaaccat atatgaatcc agtacccgcg caacgtgaat atttcctcga
    10141 ctccatccgc gcctggctga tgttgttagg gatccctttt catatttctt taatctattc
    10201 gagccataca tggcatgtga atagcgccga accatcattg tggctgaccc tttttaatga
    10261 cttcatccac tcgttccgca tgcaggtatt tttcgttata tccggctact tttcctacat
    10321 gcttttttta cgctatccct tgaaaaaatg gtggaaagta cgtgtcgaac gtgtaggtat
    10381 cccgatgtta acagccatcc ccctactgac attaccgcaa tttattatgc tgcaatatgt
    10441 caaaggaaaa gcggaaagtt ggcctgggct gtcattgtat gacaaatata atacgttggc
    10501 ctgggaatta atatcacacc tgtggttttt actggtgtta gtagtcatga cgacgctgtg
    10561 cgtatggata tttaagcgca tcagaaataa tttagaaaat tctgataaaa cgaataaaaa
    10621 attctcgatg gtaaaactat cggtgatttt tttatgcctc ggcatcggtt atgcggtaat
    10681 aagaagaacg atttttattg tgtatccgcc cattctgagt aatggcatgt tcaattttat
    10741 tgtcatgcaa acgctgtttt atttgccgtt ctttatcctc ggcgcactgg ctttcatttt
    10801 ccctcatctt aaagccttgt ttaccacgcc gtctcgtggc tgtacccttg cagcagcatt
    10861 ggcgtttgtc gcttatttac tcaaccagcg ctatggcagt ggcgatgcct ggatgtacga
    10921 aaccgagtcg gtgatcacca tggtcctcgg tctgtggatg gtgaatgtgg tcttctcctt
    10981 tggccaccgt ttgcttaact tccagtcagc gcgggtgact tattttgtta acgcatcgct
    11041 gtttatctat ctggttcacc acccgttaac gctgtttttc ggcgcataca ttacaccgca
    11101 catcacctcc aactggcttg gttttctctg tggcctgata tttgtagtag ggattgcgat
    11161 aattctgtat gaaattcatt tgcgcatccc gttactgaag tttttgttct ctggtaaacc
    11221 ggttgttaag cgtgagaacg ataaagcacc agcccgttaa gccacattta caataaccat
    11281 tccacgggca atatcgacgc cagtctgacc ataacccgct tccagaaact ggtggcgggt
    11341 tcttttttga gaataatctc tttctttgca tgacgatcaa cccagttgat ccgtccccac
    11401 ctgtccagac ggagctgcca ggccgcatca tactggctct gaataaagcg tttatcaatt
    11461 aactgtgcca gcgtttcgct ctctatcacg aagcccattt cagtattgag caatgttgaa
    11521 cgcggatcga aattgaaaga gccgataaac accgttttac catcgatgct aaaggtttta
    11581 gcatgcaggc tggctccgga attaccggtt atgccgcgat cgtgtaacgt actactttgt
    11641 tcacgcgtcg gcttgagttc atataattcc acgccatagc ggagcaattt tttgcgccag
    11701 cgcgcgtatc cggcatggac gacagcaaca tcgttagcgg caagagaatt ggttaggatc
    11761 gcaatcttta ccccttttct caccatccgt aagagttgcg ccacacctgc gcgtgtcggt
    11821 acaaaatagg aagagataat atcgatgcgt tcactgggtg agcccatgat atcgaacagg
    11881 cgctgcggta acagtgaatg ccgttttgcc ttgccctccc ctttcgccgg atcatcactt
    11941 aataaacgtg ttttcgccca gataagcggc aacgttccat caaccagatg atttataaac
    12001 ggactggatt ccattttgcg taagtaacga tgcgtcatag catcgttatg ccaggaggcg
    12061 ggtaactcga tgcgatccgc catttcacct tccgggacat ccagcacttg ctgtaagggt
    12121 gaaaccgatt tgcaatacca gtagcgggcg aaatcatcgg caacgtcctc taccactggc
    12181 cctattgcca taacatctaa atccgaaaaa agtggctctt cccctgcccc aaaataggca
    12241 tcaccaatat ttcgcccccc taccagcgtc accacgccat cgacagtgaa acttttattg
    12301 tgcatacggc gattgagacg ggaaaagtcg gtgatataac caagcggacg cagcaagcga
    12361 aacgagaaag gattaaaaag ccggacttca atgcggggat ggctgtcaag caagcgtaaa
    12421 atgtcgtcaa gtccgggcgt attgttgtcg tccagcaaca aacggacgcg aacgccacgc
    12481 tttgctgcgg ctaacagggc ggaaaacagt aaccgacccg acatatcgtc ctgccagatg
    12541 taatactgaa catccagcgt atgctctgcc atttcggcca gacgataccg ggcggcaaac
    12601 gcatccagac ttttctccag agggaaaaga ccacactgac cgggatgttg cgaacacagt
    12661 ggcagcaccg cgctcgccag ccggggcaaa tcattcatct ccttgttggg taaggagtct
    12721 ttcgtagagg tgggcgtttt cttcatcata acagacaaag tatacctgtt cgggtaaagc
    12781 gtgacgggta ataaattctg aaacggtttt aactgcgatt tcagccgctg ccgcacgagg
    12841 gtaaccataa accccagtac tgattgcagg aaaagccact gacgtatagc tgtttgccgc
    12901 cactagtcgt aggctattga gataggcatc ctgcaaaagc tggtcttcgt tttgttcacc
    12961 accacgccag actggcccga cggtgtgcac tacggcttta gcgggaagag cgcctgcaag
    13021 cgtaataacg gcatgccccg tagggcaatc gccctgctgt tgcctgactt ttaaacaggc
    13081 atccagcagg gccggacccg ctgcgcgatg aatggcccca tcgacgccgc cgcctcccat
    13141 taatgacgga ttagccgcat tcacaatcac atcaacggcc agtttggtaa tatcaccctg
    13201 cacaacatga atacgcgttt tcataacgcc tccttacacc ttcttgtttc ttaagtgtac
    13261 agcaggcttt ttggctaaat cgcatttttc tgctcagcaa cgtgcgttgt tcagaaactt
    13321 agttatattc gatgttcatt acagccagct ttttctgttt atcaagatac tgcactttta
    13381 ctgaggctat ttgcgcgttt ttaacattac cgctcattaa cgctttgctg gaataggttt
    13441 tgcccggaac agaaccattc tgtgggcagt tcatttctac gggtgatgac tgggtgctga
    13501 cgtcacatgc cggttccaca atttggccat aaaaatggat caccccccct tgcgcggtgc
    13561 ttcctgcgat ggcaataaaa ggaaaaagca aactgccgag cataagagag tttaaaaaag
    13621 gtctgaacat ttattctatt tcctcaatga ttagtcatcc ttgagggttg tgttatccat
    13681 actttcaggt tggctgattc gcagcagacc attctctcca gattcatctt atgctcgata
    13741 tttcaacaaa ttaagacttt tctgaagagg gcggccattg ttgtgataaa tgaagtgact
    13801 gtccatcaga aacagtaaca actattttca cccgatcttc cggggaaata tttaaactca
    13861 acttcgtcaa agcaataggt tgattagcag gcaatgagag ggtcttttct tgcttcgtct
    13921 gactttgccc tgaactgcct tcgcgcaggg acaatatttg tactctgcac agacaagatt
    13981 gagtaagagt gacttcagga ataatggtat acacatcccc ttgctgggtc gtattaaagg
    14041 ttatctggct ggaaagtgcc gcaaggagta ataacgcatt catattcttc tcccgaaaaa
    14101 aaacagggct tgcgccctgt ttctgtaata caaatgatgt attagtactg atgagcggtc
    14161 gcgttgttac caaagccaac ctgagtcacg ttgacggagg agttagatgc agtctggtca
    14221 actgcagcac cgttgccacc accgaactgt ttaacagtca tttcagaatt tttgccgttc
    14281 cactgatcaa gagtggcgct gttaccgaag ccacgttggg tcagatcgat tgagctgtca
    14341 tctgagccct gacctacatc tgcaccatta ccaccgccat gctgggtaat agtcaagtca
    14401 gagttacggg catcagtttg cagagcaagt gcagagttac cgccaccgta ctggtaaatg
    14461 ttcagctctg aatttgggcc gctattatta ccgccaccac cgtggttacc gccgccgccg
    14521 tactgaggaa caacacctgc cagagcgcta ccggagaata cgattgctgc aattgctgct
    14581 acttttaaaa gtttcatgta aaacccccat cggattgatt taaaagtcga atggaaatta
    14641 acgttgtgtc acgcgaatag ccatttgcga ctgtctctgc actacaattg ccgttttttg
    14701 agtaccatac tgtgtaatat ttgctttatt accagaacct ttctggataa tcatcgcagt
    14761 attaccataa gcaccttgcg aaatactggc atcgttggca ctgcccgcct gatcaatata
    14821 tgcaaggtta taatctcctg tctggtcaat ctttgcccgg ttgctactac cttcttgcgc
    14881 aacaaccgcc aaaagttttg agcctccctg ccgtaactga gcactattat tcgtcccagc
    14941 ttgaccaatt atggctgcct gattaaatga agacttactc aattcattta ccgcgaagtt
    15001 atattctgaa ttagctaaat cataacctgc tgcggctgca atcccaggcg cacccagtat
    15061 tgttaacatc ataaataaca atttgttttt catgttgtca ccctggacct ggtcgtacat
    15121 ttaagaaatt aaatcatttc aacttggttg ttaacgcaac ctgtattttg ttaacgctgc
    15181 gttacgatgg aaagtatgtc tgcggaaata tttttaataa ctcacccgcg cgttgtattt
    15241 ttcttttttt gttcatacca aagtattaaa acccacgtaa atacgctgtt atctacgcaa
    15301 aaaatatttt gttttctttt aaatctccgt tttccgctat caaaaatcac cagacagtca
    15361 tttttcttgc ccgtcgctga ttgctgcgat atgtcttgcg cacaagccgt gacaaacctc
    15421 gtctttttgc agtaaaaaat tgtccacgga ggtgcggaga aaaaacaaga acgttttaca
    15481 tgacgaaagg actacaccga aatatt
//
LOCUS       DAZGTR010000101        15248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGTR010000101 DAZGTR010000000
VERSION     DAZGTR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.102"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            248..583
                     /locus_tag="ACHLCY_003569"
     CDS             248..583
                     /locus_tag="ACHLCY_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP0370748.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            743..1186
                     /gene="yjdN"
                     /locus_tag="ACHLCY_003570"
     CDS             743..1186
                     /gene="yjdN"
                     /locus_tag="ACHLCY_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP0370749.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1319..2107
                     /gene="phnC"
                     /locus_tag="ACHLCY_003571"
     CDS             1319..2107
                     /gene="phnC"
                     /locus_tag="ACHLCY_003571"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP0370750.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIIALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2132..3148
                     /gene="phnD"
                     /locus_tag="ACHLCY_003572"
     CDS             2132..3148
                     /gene="phnD"
                     /locus_tag="ACHLCY_003572"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0370751.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3254..4033
                     /gene="phnE"
                     /locus_tag="ACHLCY_003573"
     CDS             3254..4033
                     /gene="phnE"
                     /locus_tag="ACHLCY_003573"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP0370752.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4054..4779
                     /gene="phnF"
                     /locus_tag="ACHLCY_003574"
     CDS             4054..4779
                     /gene="phnF"
                     /locus_tag="ACHLCY_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP0370753.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4780..5232
                     /gene="phnG"
                     /locus_tag="ACHLCY_003575"
     CDS             4780..5232
                     /gene="phnG"
                     /locus_tag="ACHLCY_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP0370754.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5229..5813
                     /gene="phnH"
                     /locus_tag="ACHLCY_003576"
     CDS             5229..5813
                     /gene="phnH"
                     /locus_tag="ACHLCY_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP0370755.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNALLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5813..6877
                     /gene="phnI"
                     /locus_tag="ACHLCY_003577"
     CDS             5813..6877
                     /gene="phnI"
                     /locus_tag="ACHLCY_003577"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP0370756.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLVKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6870..7715
                     /gene="phnJ"
                     /locus_tag="ACHLCY_003578"
     CDS             6870..7715
                     /gene="phnJ"
                     /locus_tag="ACHLCY_003578"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP0370757.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7712..8470
                     /gene="phnK"
                     /locus_tag="ACHLCY_003579"
     CDS             7712..8470
                     /gene="phnK"
                     /locus_tag="ACHLCY_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP0370758.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8581..9261
                     /gene="phnL"
                     /locus_tag="ACHLCY_003580"
     CDS             8581..9261
                     /gene="phnL"
                     /locus_tag="ACHLCY_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP0370759.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9258..10394
                     /gene="phnM"
                     /locus_tag="ACHLCY_003581"
     CDS             9258..10394
                     /gene="phnM"
                     /locus_tag="ACHLCY_003581"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP0370760.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CHARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10394..10951
                     /gene="phnN"
                     /locus_tag="ACHLCY_003582"
     CDS             10394..10951
                     /gene="phnN"
                     /locus_tag="ACHLCY_003582"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP0370761.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKKKHHACL"
     gene            10938..11372
                     /gene="phnO"
                     /locus_tag="ACHLCY_003583"
     CDS             10938..11372
                     /gene="phnO"
                     /locus_tag="ACHLCY_003583"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP0370762.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11374..12132
                     /gene="phnP"
                     /locus_tag="ACHLCY_003584"
     CDS             11374..12132
                     /gene="phnP"
                     /locus_tag="ACHLCY_003584"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP0370763.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12279..12608)
                     /gene="yjdP"
                     /locus_tag="ACHLCY_003585"
     CDS             complement(12279..12608)
                     /gene="yjdP"
                     /locus_tag="ACHLCY_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP0370764.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12677..12994)
                     /locus_tag="ACHLCY_003586"
     CDS             complement(12677..12994)
                     /locus_tag="ACHLCY_003586"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFP0370765.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSREVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13247..15232
                     /gene="yjcS"
                     /locus_tag="ACHLCY_003587"
     CDS             13247..15232
                     /gene="yjcS"
                     /locus_tag="ACHLCY_003587"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP0370766.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3470 a         4506 c         4187 g         3085 t
ORIGIN      
        1 cgtcagaaac ataaaaatat catgtgaatt aaaaaaaaga acaagcagag cattaacatt
       61 atcttaaata ataaatggag gcaaaaagat tattttctgt ttgcgtttcc tttcaaatga
      121 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
      181 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
      241 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
      301 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga
      361 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
      421 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
      481 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
      541 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
      601 agccggataa ggcgctcgcg ccgcatccgg caacgatgcc gactgcctga tgcgacgctg
      661 accgcgtctt atcaggcttc cctctcatct gtataaattt cgaactacac ttaactggct
      721 tctcttaact gaggtcacca tcatgccgtt aagtccctac ctctcttttg ccggtaactg
      781 tgccgacgca attgcttatt atcaacgtac gttgggcgct gaactgctct ataaaatcag
      841 cttcggcgaa atgccaaaat cagcgcagga cagcgccgag aactgccctt ccggaatgca
      901 atttcccgat accgccatcg cccacgccaa cgtgcgcatt gccggaagcg acatcatgat
      961 gagcgatgct ataccgtcag gaaaagccag ctactccggc tttacgctgg tgctcgattc
     1021 gcaacaggtc gaagaaggaa aacgctggtt tgacaatctt gccgctaacg gaaaaatcga
     1081 aatggcctgg caggaaactt tctgggcgca tggctttggc aaagtcaccg ataaatttgg
     1141 cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg caataacccg ccgggaggcc
     1201 cgccctcccg cactgtcatc gaattcccgt taactcttca tctgttagtc acttttaatt
     1261 aaccaaatcg tcacaataat ccgccacgat ggagccactt ttttagggag gctgcatcat
     1321 gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc aatcagcatc aggcgctgca
     1381 tgcggttgat ctgaacattc atcacggtga aatggtggct ctgcttgggc cgtcgggttc
     1441 cggcaaatcc acccttttac gtcacttaag cggtttgatt accggcgata aatccgccgg
     1501 cagccatatc gagctgctgg gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat
     1561 ccgcaaaagc cgcgccaaca ccggctacat cttccaacaa ttcaacctgg tgaaccgcct
     1621 gagcgtactg gagaacgtgc tgattggcgc gctcggcagc acgccgttct ggcgcacctg
     1681 ttttagctgg ttcaccggcg agcagaaaca gcgcgcgtta caggcgctga cccgcgttgg
     1741 catggtgcat tttgcccatc agcgcgtttc caccctctcc ggcggacagc agcagcgtgt
     1801 ggcgattgcc cgcgcgctga tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc
     1861 ctcgctggac ccggaatccg cccgcatcgt gatggacacc ctgcgcgaca tcaatcagaa
     1921 cgacggcatc accgtggtcg tcacgctgca tcaggtggat tacgccctgc gctactgcga
     1981 acgcatcatc gccctgcgcc aggggcacgt tttctacgac ggcagcagcc aacagtttga
     2041 taacgaacgt tttgaccatc tctaccgcag cattaatcgc atcgaagaga acgcgaaagc
     2101 tgcctgacat ccccatcatt gaggaaaacg aatgaacgct aagataattg cctcgctggc
     2161 cttcaccagc atgttcagcc tcagcaccct gttaagcccg gcacacgccg aagagcagga
     2221 aaaggcgctg aatttcggca ttatttcaac ggaatcacag caaaacctga aaccgcaatg
     2281 gacgccattc ttacaggata tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc
     2341 cccagactac gcaggcatta tccagggaat gcgcttcaat aaagtggata tcgcctggta
     2401 cggcaacctg tcggcaatgg aagcggtgga tcgcgccaac ggccaggtct tcgcccagac
     2461 ggtcgcggcg gatggatcgc caggttactg gagcgtgttg atcgtcaaca aagatagtcc
     2521 gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat ctcaccttcg gcaatggcga
     2581 tcctaactcc acctctggct tcctcgtccc cggttactac gtctttgcca aaaacaatat
     2641 ctccgccagc gactttaagc gcaccgtcaa cgctgggcat gaaaccaacg cgctggccgt
     2701 cgccaacaag caggtggatg ttgccaccaa caacaccgaa aacctcgaca agctgaaaac
     2761 ctccgcgcca gagaagctga aagaactgaa ggtgatctgg aagtcgccgc tgatcccagg
     2821 cgatccgatc gtctggcgta aaaacctttc cgaaaccacc aaagacaaga tctacgactt
     2881 ctttatgaac tacggcaaaa cgccggaaga aaaagcggtg ctggaacgcc tgggctgggc
     2941 gccgttccgc gcttccagcg acctgcaact ggtgccgatt cgccagctcg cgctgtttaa
     3001 agagatgcag ggcgtgaaaa gcaataaagg actgaatgag caggacaagc tggcaaaaac
     3061 caccgagatt caggcgcagc tggatgacct ggaccgcctg aacaacgcgt taagcgcgat
     3121 gagttcggtg agtaaagcgg tgcagtaaat cgtaggtcgg ataagacgcc ccggcgtcgc
     3181 atccgacaat gtgcaggcgt tgatgccgga tgcggtgcaa gcaccttatc cggcctacag
     3241 accggagcca aacatgcaaa ccatcaccat cgccccaccc aagcgcagct ggttctcgct
     3301 tctgagctgg gccgttgttc tcgccgtgtt ggtcgtctcg tggcagggcg cggaaatggc
     3361 cccgctcacg ctgattaaag acggcggcaa catggcgacg ttcgccgccg acttcttccc
     3421 gcccgatttc agccagtggc aggattacct caccgaaatg gccgtcacgc tgcaaatcgc
     3481 cgtctggggc acggcgctgg cggtggttct ctccatcccc ttcggcctga tgagcgccga
     3541 aaacctggtg ccgtggtggg tttaccagcc cgttcgccgc ctgatggacg cctgccgcgc
     3601 cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc gtcggcctcg gcccgttcgc
     3661 tggcgtgctg gcgctgttta tccacaccac cggcgtgctc tccaaactgc tttccgaagc
     3721 ggtggaagcg attgaacccg gcccggtgga aggcattcgc gccaccggcg ccaacaagct
     3781 cgaagagatc ctctacggcg tgctgccgca ggtgatgccg ctgctgatct cctactccct
     3841 ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc ggcatggtcg gtgcaggcgg
     3901 gatcggcgtc accctgtggg aagcgattcg cggtttccag ttccaacaaa cctgcgccct
     3961 gatggtgctt atcatcgtca cggtcagcct gctggatttc ctctctcaac ggttgcgtaa
     4021 gcactttatc tgataagcga ggcattgata tctatgcact tgtctacaca tccgaccagc
     4081 tacccaacac gctatcagga gatagccgca aaacttgagc aggagcttcg tcaacactac
     4141 cgctgcggcg actatcttcc cgccgagcag caactggcgg cgcgctttga ggtgaaccgc
     4201 cacaccctgc gccgcgccat cgaccaactg gtggaaaaag gctgggtaca gcgccgtcag
     4261 ggcgtcggcg tgctggtgct gatgcgcccg ttcgattacc cgctcaacgc ccaggcgcgt
     4321 tttagccaga acctgctgga ccagggtagc catcccacca gcgaaaaact gctgtcggta
     4381 ttgcgccccg cgtccggtca cgtcgctgac gcgctgggga ttaccgaggg cgagaacgtt
     4441 atccatttgc gcaccctgcg tcgcgtcaac ggcgtcgcgc tctgtttaat cgaccactac
     4501 tttgcggacc tcaccctttg gccgacgctg caacgcttcg acagcggttc gctgcacgat
     4561 tttctgcgcg agcaaaccgg aattgcgctg cgccgcagcc agacgcggat cagcgcccgt
     4621 cgcgcccagg ccaaagagtg ccagcgtctt gaaattccga atatgtcgcc gctgctgtgc
     4681 gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg cggagtactc cgtcagcctg
     4741 acgcgcgccg acatgattga attcactatg gagcactgaa tgcacgcaga taccgcgacc
     4801 cgccagcact ggatgtccgt actggcgcac agccaaccgg ctgaactggc agcccgcctg
     4861 aaagcactga acatcaccgc cgactatgag gttatccgcg ccgccgaaac cggcctggtg
     4921 cagattcagg cgcggatggg cggcaccggc gaacgctttt ttgccggcga cgccacgctc
     4981 acccgcgccg ccgtacgcct gactgacggc acgctcggct acagctgggt gctggggcgc
     5041 gacaaacagc acgccgaacg ctgcgcgctg attgacgcgc tgatgcagca atctcgctat
     5101 tttcaaaact tatcagaaac ccttattgcc ccgctggacg ctgaccgtat ggcacgcatt
     5161 gccgcacgcc aggccgaagt gaacgccagc cgggtcgact tctttacgat ggttcgcgga
     5221 gacaacgcat gaccctggaa accgctttta tgcttcccgt gcaggatgcc cagcacagtt
     5281 ttcgtcgcct gttaaaggcc atgagcgagc cgggcgtgat tgtcgccctg catcagctca
     5341 agcgcggctg gcagccgctg aatatcgcca ccaccagcgt gctgctgacg ctggccgata
     5401 acgacacgcc ggtgtggctt gccgcgccat tgagtaacga tatcgtcagc cagagcctgc
     5461 gttttcatac caacgcgctg ctggtcagcc agccggaaca ggcgaccttc gcggtgacgg
     5521 atgaggcgat ttccagcgaa cagcttaacg ccctttccac cggcaccgcc gttgcgccgg
     5581 aagcgggtgc aacgctgatt ttacaggtcg ccagcctgag cggcgggcgc atgttacgtc
     5641 tcaccggcgc gggcattgcc gaagaacgaa tgatcgctcc gcagctgccg gagtgcattc
     5701 tgcacgaact caccgagcgc ccgcacccgt tcccgctcgg catcgacctg atcctgacct
     5761 gcggcgagcg cctgctggct attccgcgaa ccactcatgt ggaggtgtgc tgatgtacgt
     5821 tgccgtgaaa gggggcgaga aggcgatcga cgccgctcac gccctgcaag agagccgacg
     5881 ccggggcgat accgatttgc ctgaactgag cgtcgcccag attgaacagc agcttaatct
     5941 tgcggtggat cgcgtgatga ccgaaggcgg cattgccgac cgcgaactgg cggcgctggc
     6001 gctgaaacag gccagcggcg ataacgtcga agcaattttc ctgctgcgcg cctaccgcac
     6061 cacgctggcg aagctcgcgg taagcgagcc gctcgacacc acagagatgc gtctcgaacg
     6121 gcgtatttcg gcggtttata aagacattcc cggcggccag ctgcttggcc caacctacga
     6181 ctacacccat cgcctgctcg atttcaccct gctggcaaac ggtgaagcgc cgacgctgac
     6241 caccgccgac agcgaacaac agccgtcgcc gcacgttttc agcctgctgg cgcgtcaggg
     6301 gctggtgaag tttgaagaag atagcggcgc acagccggat gacatcaccc gcacgccgcc
     6361 ggtttacccc tgctcacgct cctcccgcct gcaacaatta atgcgcgggg acgaaggcta
     6421 tttgctggcg ctggcctact ccacccagcg tggttacggg cgcaatcacc cgttcgcggg
     6481 cgagatccgc agtggttaca tcgacgtgtc gattgtgccg gaagagctgg gatttgcggt
     6541 aaacgtcggc gaactactga tgaccgagtg tgaaatggtc aacggtttta tcgacccgcc
     6601 ggatgagccg ccgcacttca cgcgcggcta cgggctggta ttcggcatga gcgagcgcaa
     6661 agcgatggca atggcgctgg tcgatcgtgc gttgcaggct ccggaatacg gcgagcacgc
     6721 gacaggcccg gcgcaggatg aagagtttgt gctggcacat gccgacaacg tcgaagccgc
     6781 aggctttgtc tcgcacctca aactccccca ctacgtcgat ttccaggccg aactggagct
     6841 actcaaacgt ctgcaacagg agaagaacca tggctaatct gagcggctac aactttgcct
     6901 acctcgacga gcagaccaaa cgcatgatcc gccgcgctat cttaaaagcg gtggcgatcc
     6961 ccggttatca ggtgccgttt ggcgggcgcg agatgccgat gccatacggc tggggaaccg
     7021 gcggcataca gctcaccgcc agcgtgattg gcgaaagcga cgtgctgaag gtgattgacc
     7081 agggtgcgga tgacaccacc aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg
     7141 gggtaaacac cactgaacgt acggacgatg cgacgcttat ccagacgcgt caccgcatcc
     7201 ccgaaacgcc gctgaccgaa gatcagatca ttatcttcca ggtgccaatc ccggaaccgc
     7261 tgcgctttat cgagccgcgc gaaacggaaa cccgcaccat gcacgcgctg gaagagtacg
     7321 gcgtgatgca ggtgaaactg tatgaagata tcgcccgctt cggtcatatc gccactacct
     7381 acgcctatcc ggtgaaggtg aacgggcgct acgtaatgga cccgtcgccg atcccgaaat
     7441 tcgataaccc aaaaatggac atgatgcccg ccctgcaact gttcggcgcg gggcgcgaga
     7501 agcgcatcta tgcggtgccg ccgtttaccc gcgtggaaag tctcgatttc gacgatcacc
     7561 cgttcaccgt tcagcagtgg gatgagccat gcgccatctg cggatcgacc cacagctatc
     7621 ttgatgaagt ggtgctggat gacgccggaa accgcatgtt tgtctgctcc gataccgatt
     7681 attgccgcca acagagcgag gcaaaaaacc aatgaatcaa ccgttacttt cggtcaataa
     7741 cctgacccac ctttacgcgc cgggcaaagg ctttagcgat gtctcttttg atttatggcc
     7801 gggggaagta ctgggcattg tcggggagtc cggctccggg aagaccacgc tgctgaaatc
     7861 aatctccgcg cgcctgacgc cgcagcaggg ggagattcac tatgagagcc gttcgctgta
     7921 cgcaatgagc gaggccgacc gccgtcgcct gctgcgcacc gaatggggcg tagtgcatca
     7981 gcatccactc gacggcctgc gccgtcaggt ctcggcaggc ggcaatatcg gcgagcggct
     8041 gatggcgacc ggggcacgtc attacggcga tattcgtgcc accgcgcaga agtggctgga
     8101 agaggtggaa attcccgcca accggatcga cgacctgccg accacctttt ccggcggcat
     8161 gcagcaacgt ttgcagatcg cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga
     8221 cgagccgacc ggcggactgg atgtgtcggt acaggcacgc ctgctcgacc tgctgcgcgg
     8281 cctggtggtg gagctgaacc tcgcggtagt gattgtcacc cacgatttag gcgtcgcccg
     8341 cctgctggcg gaccgtttgc tggtgatgaa gcaggggcaa gtggtggaga gtgggttaac
     8401 cgaccgcgtg ctcgacgacc cgcaccatcc gtatacacaa ctgctggtgt catcggtttt
     8461 gcagaattga gccggtgccg gatgcggcgt aaacgcctta tccggcctac aaatgcgctc
     8521 cccgtaggtc ggataagacg cgtcagcgtc gcatccgaca cccgaaccac gaggcgaaaa
     8581 atgattaacg tacaaaacgt cagtaaaacc ttcatcctgc accagcaaaa cggcgtgcgc
     8641 ctgcccgtct tacaacgtgc ctcgctcacc gtcaacgcgg gcgaatgcgt ggtgctccac
     8701 ggccattccg gcagcggcaa atcaaccttg ctgcgctcgc tgtacgccaa ctatctgccc
     8761 gacgaagggc aaatccagat caaacacggt gacgaatggg tcgatctggt caccgcgcca
     8821 gcgcgcaaag tggtggagat ccgcaaaacc accgtcggct gggtgagcca gtttctgcgc
     8881 gtcatcccgc gtatttcagc actggaagtg gtgatgcagc cgctgctcga taccggcgtt
     8941 ccgcgtgaag cctgcgccgc taaagccgcg cgtctgctca cccgcctgaa cgtgccggaa
     9001 cgcctgtggc acctggcgcc atcaaccttt tccggtggcg aacagcagcg cgtcaacatc
     9061 gcccgcggct ttatcgtcga ctaccccatt ctgctgctcg acgaacctac cgcctcgctg
     9121 gacgccaaaa acagcgccgc cgtagtcgag ctgattcgcg aagccaaaac ccgtggcgcg
     9181 gccatcgtcg gcatcttcca tgacgaagcc gtacgtaatg acgtcgccga ccgcttgcac
     9241 ccaatgggag cctctgcatg attatcaata acgtcaaact ggtgctggaa aacgaagtgg
     9301 tacacggttc gctggaggtg caggatggcg aaatccgcgc ctttgccgaa agccagagcc
     9361 gcctgccaga agcgatggac ggcgaaggtg gctggctact acccggcctg attgagctgc
     9421 ataccgacaa cctcgataaa ttcttcaccc cgcgcccgaa agtcgactgg cccgcccatt
     9481 cggcgatgag cagccacgac gcgctgatgg tggcaagcgg catcaccacc gtactggacg
     9541 ccgtggcgat tggcgacgtg cgcgacggcg gcgatcggct ggagaatctg gagaagatga
     9601 tcaacgccat cgaagagacg caaaaacgcg gcgtcaaccg cgccgagcac cgcctgcacc
     9661 tgcgctgcga actgccgcat cacaccacac tgccgctgtt tgaaaaactg gttcagcgcg
     9721 aaccggtgac gctggtgtcg ctgatggacc actcaccggg ccagcgccag ttcgctaacc
     9781 gcgagaagta tcgcgaatat tatcagggca aatactccct caccgatgcg cagatgcagc
     9841 agtacgaaga agagcaattg gcgctcgccg cacgctggtc gcagccgaat cgcgaatcca
     9901 tcgccgccct gtgccacgcg cgacaaattg cgctcgccag ccacgatgac gccacgcacg
     9961 cccacgttgc cgaatcccac caacttggca gcgtgatcgc cgaatttccc accacgttcg
    10021 aagcggcgga agcctcgcgt aagcatggca tgaacgtgct gatgggtgcg ccaaatatcg
    10081 tgcgtggcgg ctcgcactcc ggcaatgtgg cggccagtga actggcgcag cttggtctgc
    10141 tggatatcct ctcttccgac tactaccccg ccagcctgct ggatgcggcg ttccgcgtcg
    10201 ccgatgacga gagcaaccgc tttacgctac cgcaggcggt gaggctggtg accaaaaatc
    10261 cggcgcaggc gctgaatctt caggatcgcg gggtgattgg cgagggtaaa cgcgctgacc
    10321 tggtgctggc gcatcgccag ggcaatcaca ttcatatcga ccacgtctgg cgtcagggta
    10381 aaagggtgtt ctgatgatgg gaaaactgat ttggttaatg gggccgtccg gctccgggaa
    10441 agacagcctg ctggcggaac tccgcctgcg ggaacaaacg cagttactgg tggcgcatcg
    10501 ctacatcacg cgcgacgcca gcgccggaag tgaaaaccat atcgccctga gtgagcggga
    10561 gttttttacc cgcgcggggc aaaacctgct ggccttaagc tggcacgcta acggtctgta
    10621 ttatggcgtc ggcatcgaga ttgacctctg gctgcacgct ggattcgacg tagtcgtcaa
    10681 cggttcacgc gcgcatttgc cgcaggcacg ggcgcgctat caatcggcgc tgctgcccgt
    10741 ctgtttacag gtttcgccgg agatcctgcg ccagcgcctg gaaaaccgtg gccgtgaaaa
    10801 cgccagtgaa attaacgccc ggctggcgcg cgccgcccgc tatactccac aggattgcca
    10861 tacgctcaac aatgacggca gcctgcgcca gtcggtcgac acactgctga cgctgattca
    10921 tcagaagaag aaacaccatg cctgcttgtg agcttcgccc ggccacgcag tacgataccg
    10981 acgcggttta cgcgctgatt tgtgagctaa aacaggcgga gtttgaccac cacgcgtttc
    11041 gcgtgggctt taacgccaac ctgcgcgacc cgaacatgcg ctaccatctg gcgctgctgg
    11101 atggcgaagt tgtcggcatg atcggcctgc atttgcagtt tcatctgcat catgtcaact
    11161 ggatcggcga aattcaggaa ctggtggtga tgccgcaggc gcgcggcctg aacgtcggta
    11221 gcaagttgct ggcgtgggca gaagaagaag cccgccaggc cggggccgaa atgaccgagc
    11281 tttcgaccaa cgtgaagcgc cacgacgcgc accgtttcta tctgcgagaa ggctacgagc
    11341 agagccactt ccgcttcacc aaggcgctgt aacatgagcc tgaccctcac gctcaccggc
    11401 actggcggcg cacagggcgt tccggcatgg ggctgcgagt gcgcggcctg cgccagagcg
    11461 cggcgctcgc cgcagtatcg ccgccagcca tgcagcggcg tagtgaagtt taacgacgcg
    11521 atcaccctga tcgacgccgg gcggcacgat ctcaccgatc gctggtcgcc tggatcgttc
    11581 cagcagtttt tgctgacgca ctaccatatg gatcacgtcc aggggctatt tccgctacgc
    11641 tggggcgtgg gcgatgtgat cccggtctac ggcccgccgg atgaacaggg ctgcgacgat
    11701 ctgtttaaac atccgggcct gcttgatttc agccacacag tggaaccgtt tgtggtgttt
    11761 gatttgcagg ggttgcaggt cacgccgctg ccgcttaacc actcaaaact gaccttcggt
    11821 tatctgctgg aaacggcaca tagccgggtg gcgtggctgt ctgacaccgc tggcctgccg
    11881 gaaaaaacgc tgaaattttt actcaataat catccgcagg tgatggtgat tgattgcagt
    11941 cacccgccgc gcgcggatgc accgcgtaat cactgtgatt taaataccgt gcttgcgctg
    12001 aatcaggtta tccgctcgcc acaggtgatt ctgacccata tcagccacca gtttgatgcg
    12061 tggctgatgg aaaacgcact accgtcaggg tttgaggtgg ggtttgatgg gatggagatt
    12121 ggggtggcgt gatgagaggg aatgtgcgcg ctggccccct caccctaacc ctctccccag
    12181 aggggcgagg ggaccgattg tgctcaatat tgaatattgc gctcgttttc tccctctccc
    12241 cattggggtg aggggcgatg cctgctccat acccaacctc atcgcccata ctcatcttcc
    12301 attctccgct cttcatcctc cagttgccga cgctcctgat caagctggcg ctggcgatcg
    12361 tccagctgcc tgcggcgatc ttcaaactgg cggcggcggt cgtcatattg tctgcgccga
    12421 tcgtcgctca cttcacgctg ccagccgtcg tcgcgcgaat cttcatagtc tcgcccacgg
    12481 tcagggttat aagcgtcatt aatcgcctgc tgaatattac caatagtatc gtcgataata
    12541 tcagcctggg ccggaacggt ggacagcgtg agcagggtga ataaaagaaa tagcggaaag
    12601 cgtttcatta gccaacctca aaaagaaact ctatccacat tagtcattac tcatccatgc
    12661 aagtagtgga tgaatctcaa tttctccgct gctctattgc cgtaatcgcc tccacgcgtt
    12721 gttgatgacg accgccttcg tactgtgcgc ccagccacgc atccacaatc atttttgcca
    12781 gttcgaggcc aacctctcgt gaaccaaaag ccagtgcaac ttcactggca taatgcggat
    12841 aatcagtgcg ctctgacgac ctggtgtctt tatcaatcac ctcactcatt gaccatgaat
    12901 aaaaggctgg cacagcaaca cagaatcctg cggtgccagc gacatgaccg cccattgctc
    12961 cggtacgttt tttaaaaaga aacaccggtt gcattgataa attgtgctca gtatttgccg
    13021 aagtacaata tatataataa atatataaaa cacctcatat tatttacaga gataataaat
    13081 tcgcgtaatt ttcccccttc agcacaaatg cctaataaaa tggatcgttc cgctcacact
    13141 tccacttatt aaagtcgcaa attcccaatt atgataacgc cagctttaaa tgacgtattt
    13201 tgcatggatt acatatttac gacacaaatg ataaatggag atttatatga ataactctcg
    13261 gttattccgt ttgagcagga ttgttattgc gttaactgct gccagcggca tgatggtaaa
    13321 taccgctaac gcgaaagagg aagcgaaagc cgccactcaa tatacccaac aggttaatca
    13381 gaattacgcc aaatcattac cgtttagcga tcgtcaggat tttgacgacg ctcagcgtgg
    13441 atttatcgct ccgctgctgg atgaaggtat tctgcgcgat gctaatggca aaccatatta
    13501 tcgcggggaa gattataaat ttgatatcaa tgcgcccgcg cccgaaaccg ttaaccctag
    13561 cctgtggcgt cagtcgcagt taaacggtat ttccggcctg tttaaagtca ccgacagaat
    13621 gtaccaggtt cgcggtcagg atatctccaa catcaccttt atcgaaggtg ataccgggat
    13681 tattgtcatc gacccgctgg tgacgccacc gagcgcaaaa gccgcccttg acctttactt
    13741 ccaaaatcgc ccgcaaaaac cgattgttgc ggttatttat acccacagcc ataccgacca
    13801 ctatggcggc gtgaaaggca ttatctctga agctgatgtg aaatccggta aagtgcaggt
    13861 tatcgcccct gctggcttta tggacgaagc catcagcgaa aacgtactgg cgggtaatat
    13921 tatgagccgc cgtgcacttt actcctacgg cctgctgctg gcgcataacc ctcagggtaa
    13981 catcggcaat ggtcttggcg taacgctggc atcgggctac ccgagcatca tcgcaccgaa
    14041 caaaaccatc accaaaaccg gtgagaagat gattatcgac ggcctggagt ttgacttcct
    14101 gatgacccca ggtagcgaag caccagccga aatgcacttc tatattccgg ccctgaaagc
    14161 gctgtgtacc gccgagaacg ccacgcatac cctgcacaac ttctacactc tacgcggcgc
    14221 gaaaacccgc gacaccagca agtggaccga gtatctgaac gaaacgctgg atatgtgggg
    14281 taacgacgcg gaagtcctgt ttatgccgca cacctggccg gtctggggca ataagcatat
    14341 caatgattat attggtaaat atcgcgatac tatcaagtac attcacgacc agaccctgca
    14401 cctggcgaac cagggctaca ccatgaatga aatcggcgac atgattaaac tgccgcctgc
    14461 acttgccaat aactgggcca gccgtggcta ttacggttct gtcagccaca acgcccgcgc
    14521 ggtgtataac ttctatcttg gctattacga cggtaacccg gctaacctgc atccgtatgg
    14581 tcaggtggag atgggtaaac gttacgtgca ggcgctgggc ggttctgccc gtgtcatcaa
    14641 cctggcgcaa gaagcgaaca agcaaggtga ttaccgctgg tcggcagaac tgctgaaaca
    14701 ggtgattgcc gccaacccgg gtgaccaggt cgcgaagaat ctgcaagcga ataactttga
    14761 acagctgggc tatcaggccg agtccgccac ctggcgcggt ttctacctga ccggcgcgaa
    14821 agagctgcgc gaaggggtgc ataagttcag ccacggcacc accggttccc cggacaccat
    14881 tcgcgggatg tcggtcgaaa tgctgttcga ctttatgtcc gttcgcctcg atagcgcgaa
    14941 agccgcgggt aaaaatatca gcctgaactt caatatgagc aatggcgata acctcaacct
    15001 gacgctgaac gatagcgtgc ttaactaccg taaaacactg caatcccaag ctgacgcctc
    15061 tttctacatc agccgtgaag atctgcacgc cgtgctgacc gggcaagcca aaatggcgga
    15121 tctggtaaaa gcgaagaaag ccaaaattat tggcaatggc gcgaaactgg aagaaattat
    15181 cgcctgtctg gataatttcg atttgtgggt gaatatcgta accccaaatt aatgtcactc
    15241 gcttttgg
//
LOCUS       DAZGTR010000102        15169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGTR010000102 DAZGTR010000000
VERSION     DAZGTR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.103"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(85..171)
                     /locus_tag="ACHLCY_003588"
     tRNA            complement(85..171)
                     /locus_tag="ACHLCY_003588"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Leu,seq:cag)
     gene            complement(439..1470)
                     /gene="rsmC"
                     /locus_tag="ACHLCY_003589"
     CDS             complement(439..1470)
                     /gene="rsmC"
                     /locus_tag="ACHLCY_003589"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP0370767.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1573..1986
                     /gene="holD"
                     /locus_tag="ACHLCY_003590"
     CDS             1573..1986
                     /gene="holD"
                     /locus_tag="ACHLCY_003590"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP0370768.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1955..2401
                     /gene="rimI"
                     /locus_tag="ACHLCY_003591"
     CDS             1955..2401
                     /gene="rimI"
                     /locus_tag="ACHLCY_003591"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0370769.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGECYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2416..3093
                     /gene="yjjG"
                     /locus_tag="ACHLCY_003592"
     CDS             2416..3093
                     /gene="yjjG"
                     /locus_tag="ACHLCY_003592"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP0370770.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3184..4773
                     /gene="prfC"
                     /locus_tag="ACHLCY_003593"
     CDS             3184..4773
                     /gene="prfC"
                     /locus_tag="ACHLCY_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP0370771.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEGLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            5166..5771
                     /gene="osmY"
                     /locus_tag="ACHLCY_003594"
     CDS             5166..5771
                     /gene="osmY"
                     /locus_tag="ACHLCY_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP0370772.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5898..6059
                     /locus_tag="ACHLCY_003595"
     CDS             5898..6059
                     /locus_tag="ACHLCY_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP0370773.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6181..7254
                     /locus_tag="ACHLCY_003596"
     CDS             6181..7254
                     /locus_tag="ACHLCY_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP0370774.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7251..8033
                     /locus_tag="ACHLCY_003597"
     CDS             7251..8033
                     /locus_tag="ACHLCY_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0370775.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(8146..9009)
                     /locus_tag="ACHLCY_003598"
     CDS             complement(8146..9009)
                     /locus_tag="ACHLCY_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP0370776.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEGVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(8981..10531)
                     /locus_tag="ACHLCY_003599"
     CDS             complement(8981..10531)
                     /locus_tag="ACHLCY_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP0370777.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITHAILRADADLKQVSPNLTFIYDPEITPDDLLLKVAKNICECSKPHIAN
                     GPVHDKFFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            10789..11568
                     /gene="deoC"
                     /locus_tag="ACHLCY_003600"
     CDS             10789..11568
                     /gene="deoC"
                     /locus_tag="ACHLCY_003600"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP0370778.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11695..13017
                     /gene="deoA"
                     /locus_tag="ACHLCY_003601"
     CDS             11695..13017
                     /gene="deoA"
                     /locus_tag="ACHLCY_003601"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP0370779.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            13069..14292
                     /gene="deoB"
                     /locus_tag="ACHLCY_003602"
     CDS             13069..14292
                     /gene="deoB"
                     /locus_tag="ACHLCY_003602"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0370780.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14372..15091
                     /gene="deoD"
                     /locus_tag="ACHLCY_003603"
     CDS             14372..15091
                     /gene="deoD"
                     /locus_tag="ACHLCY_003603"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP0370781.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3601 a         3960 c         4113 g         3495 t
ORIGIN      
        1 tccgtaagaa cactaacacc tgaagctagc acgtctacca attccgccac cttcgcccag
       61 tgcgagcaat atcaacgtgg tttttggtgc gagggggggg acttgaaccc ccacgtccgt
      121 aaggacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cataccatca
      181 attcttaaaa agaattgcta ccacggaggc gcattctagt ggttttcagc ttttcgtcaa
      241 tagttaatta tcgccggagg tgtaattgct ggaaaaatgt ccatcaggaa actagcgtgc
      301 aggtttggta ggcatgcggg ggcagatgcc agatgcgacg ctggcgcgtc ttatctggcc
      361 tacgaagggc taacgtgcag gttttgtagg tcggataagg cgttcacgcc gcatccgaca
      421 cggtattcgg cgagataatt aacctttctt cgcctggcgg gtcataatgg cgcgatacac
      481 cttgaaacgc ccggtttgcg caatcacttc atggaagcca aatgtctcat ccagcacgtc
      541 cgggtaaggc aggaaggcgt tcgctacaat tcgcagctcg ccgccgctat taagatgacg
      601 caccgcgccg cgaatcagcg tttgcgccgc atccaggctg gtttgcatcc catcgtggaa
      661 cggcgggttg gagatgatca tatcaaaacg acctttcacc tcggaaaaga cgttgctggc
      721 aaagacttca ccttcaacac agttggccgc aagtgttgcg cggctggctt ctaccgctgg
      781 cgcagagaca tcgcacaagg tgagacgaat tttcggcgaa tggcgcgcaa aggcaactga
      841 aagcaccccc gcgccacagc cgacatccag cactttacct ttcgtgtgcg gagttaacgt
      901 cgagagcagc aactggctac cgacatccag accgtcgcgg ctaaacacgc caggcagcgt
      961 tttgaccgtc aggccatcga cgctgtattc gccccagaat ttatcggcat caaataccgg
     1021 ctgtttttcc agacgaccaa aatagaggcc acagcgacga gcgctgtcga ctttattcaa
     1081 cggcgcataa tctgccagca tctgctcggc gctgcgcacg ccgctgcggt tctcgccaac
     1141 gacaaatata tctgtcccca ctggcagcag agaaagtaaa ttcatcaact ggaactgggc
     1201 ttccggtttg ttcttcggcc agtagtaaat cagcgtatcg caatctgcga cgtcatccac
     1261 cgtggcgacc agactgaaac gggcgttatc ccccatctgg cggcttaata cctgccagtg
     1321 gtggaattgc tgggtatgag cacggctggc cgcggtatct aaacgcgcgg gcaggtcatc
     1381 ctgtaagtct ccggcaaaca gaatacggct ttgttcgaaa tcatcactgt gacgcagcaa
     1441 gacttcactt gccggggtaa atgcagacat ggaatgctcc tcaattgata ctggcggcga
     1501 ttatagccat atgttggcgc ggtatcgacg aatttgctat atttgcgccc ctgacaacag
     1561 gagcgattcg ctatgacatc ccgacgagac tggcagttac agcaactggg cattacccag
     1621 tggtcgctgc gtcgccctgg cgcgttgcag ggggagattg ccattgcgat cccggcacac
     1681 gtccgtctgg tgatggtggc aaacgatctt cccgccctga ccgatccttt agtgagcgat
     1741 gttctgcgcg cattaaccgt cagccccgat caagtgctgc aactgacgcc agaaaaaatc
     1801 gcgatgctgc cgcaaggcag tcgctgcaac agctggcggt tgggtactga cgaaccgcta
     1861 tcactggaag gcgctcaggt ggcatcaccg gcgctcaccg aattacgggc aaacccaacg
     1921 gcacgcgccg cgttatggca acagatttgc acatatgaac acgatttctt ccctcgaaac
     1981 gactgattta ccagcggctt accacattga acaacgcgcc cacgcctttc cgtggagtga
     2041 aaaaaccttt gccagcaacc agggcgagtg ttatctcaac tttcagttaa cgcaaaacgg
     2101 caaaatggcg gcgtttgcga ttacgcaagt ggtgctggat gaagctacat tgttcaatat
     2161 tgcggtcgat cctgactatc agcgtcaggg attgggaagg gtgctgctgg aacatctgat
     2221 cgacgaactg gaaaaacgcg gcgtggcgac tctctggctg gaagtccgtg cctcaaatgc
     2281 tgccgccatt gccctgtacg aaagtttagg ctttaacgag gcgacgattc gccgcaatta
     2341 ctaccccacc acggacggtc gcgaagacgc catcatcatg gcgttgccaa tcagtatgta
     2401 agacaaggtg gaataatgaa gtgggactgg attttctttg atgccgatga aacgctgttt
     2461 acctttgact cattcaccgg cctgcagcgg atgtttcttg attacagcgt cacctttacc
     2521 gctgaagatt ttcaggacta tcaggccgtt aacaaaccac tgtgggtgga ttatcaaaac
     2581 ggcgcgatca cttcattaca gcttcagcac gggcgtttcg agagctgggc cgaacggctg
     2641 aaagttgaag caggcttgct taacgatgcc tttattaatg cgatggcgga aatctgcacg
     2701 ccgctgccgg gcgcggtttc tctgcttaac gccattcgtg gcaacgccaa aatcggcatc
     2761 atcaccaacg gctttagcgc cttgcagcaa gtgcgtctgg aacgcacggg cctgcgtgat
     2821 tacttcgatt tgctggtgat ttccgaagaa gttggcgttg ccaaaccgaa taagaaaatt
     2881 ttcgattatg cgctggaaca ggcgggcaat cctgaccgtt cacgcgtgct gatggttggc
     2941 gacactgctg agtccgatat tctcggtggc atcaacgccg ggcttgcgac ctgctggctg
     3001 aatgcgcaca atcgcgagca accagaaggc atcgcgccca cctggaccgt ttcatcgttg
     3061 cacgaactgg agcagctcct gtgtaaacac tgattgcctc ccccccgttg atgggtaaaa
     3121 tagccgcaat ttttcgtttt caacaagcgc ggcgcgatgc cgcttactca agaagaaaga
     3181 attatgacgt tgtctcctta tttgcaagag gtggcgaagc gccgcacttt tgccattatt
     3241 tctcacccgg acgccggtaa aactaccatc accgagaagg tgctgctgtt cggacaggcc
     3301 attcagaccg ctggtacagt aaaaggccgt ggttccaacc agcacgctaa gtcggactgg
     3361 atggagatgg aaaagcagcg tgggatctcc attactacgt ctgtgatgca gtttccgtat
     3421 cacgattgcc tggttaacct gctcgacacc ccggggcacg aagacttctc ggaagatacc
     3481 tatcgtaccc tgacggcggt ggactgctgc ctgatggtta tcgacgctgc aaaaggtgtt
     3541 gaagatcgta cccgtaagct gatggaagtt acccgtctgc gcgacacgcc gatcctcacc
     3601 tttatgaaca aacttgaccg tgatatccgc gacccgatgg aactgctcga tgaagttgag
     3661 aacgagctga aaatcggctg cgcaccgatc acctggccga ttggctgcgg caagctgttt
     3721 aaaggcgttt accaccttta taaagacgaa acctatctct atcagagcgg taaaggccac
     3781 acgattcagg aagtccgtat tgttaaaggg ctgaataacc cggatctcga cgctgcggtt
     3841 ggtgaaggtc tggcacagca gctacgtgac gaactggaac tggtgaaagg cgcgtctaac
     3901 gagttcgaca aagaattgtt ccttgcgggc gaaatcactc cggtattctt cggtactgcg
     3961 ctgggtaact tcggcgttga tcatatgctg gatggcctgg tggagtgggc tcctgcgccg
     4021 atgccgcgtc agactgatac ccgtaccgta gaggcgagcg aagacaaatt taccggcttc
     4081 gtatttaaaa ttcaggccaa catggacccg aaacaccgcg accgcgtggc gtttatgcgc
     4141 gtagtatccg gtaaatatga aaaaggcatg aagctgcgcc aggtgcgtac tgcgaaagat
     4201 gtggttatct ccgacgcgct gacctttatg gcgggcgacc gttcgcacgt tgaagaagcg
     4261 tatccgggcg atatcctcgg cctgcacaac cacggcacta ttcagatcgg cgacaccttt
     4321 acccagggtg agatgatgaa gttcaccggt attccgaact tcgcaccaga actgttccgt
     4381 cgtatccgcc tgaaagatcc gctgaagcaa aaacagctgc tcaaagggct ggtacagctt
     4441 tccgaagagg gcgcggtgca ggtgttccgt ccgatctcca acaacgattt gatcgttggt
     4501 gctgttggtg tgctgcagtt tgatgtggtg gtatcgcgcc tgaagagcga atacaacgtt
     4561 gaagcagtat atgaatcagt caacgttgcc actgcccgct gggtagaatg tgcggacgcg
     4621 aagaaattcg aagagttcaa gcgtaagaac gaaagccaac tggcgcttga tggcggcgat
     4681 aacctcgctt acatcgctac cagcatggtc aacctgcgcc tggcgcagga acgttatccg
     4741 gacgttcagt tccaccagac ccgcgagcat taattcttgc ctccagggcg cggtagccgc
     4801 tgcgccctgt caatttccct tccttattag ccgcttacgg aatgttctta aaacattcac
     4861 ttttgcttat gttttcgctg atatcacgag cggtttcaaa attgtgatct atatttaaca
     4921 aagtaatgac atttctgacg gcgttaaata ccgttcaatg cgtagatatc agtatctaaa
     4981 gccgtcgatt gtcattctac cgatattaat aactgattca gaggctgtaa tggtcgttat
     5041 tcatcaccca tcgcttttgt gatggcgacc attgacttct gtagagggtg aagtctctcc
     5101 ctattcagca atgcaacctc gtgttgccag gctcaaatta cgagcaaaca tacaggaata
     5161 aatcgatgac tatgacaaga ctgaagattt cgaaaactct gctggctgta atgttgacct
     5221 ctgccgtcgc gaccggctct gcctacgcgg aaaacaacgc acagactacc aatgaaagcg
     5281 cagggcaaaa agtcgatagc tctatgaata aagtcggtaa tttcatggat gacagcgcca
     5341 tcaccgcgaa agtgaaggcg gcgctggtgg atcatgacaa catcaagagc accgatatct
     5401 ctgtcaaaac cgatcaaaaa gtcgtgaccc tgagcggttt cgttgaaagc caggctcagg
     5461 ccgaagaggc agtgaaagtg gcgaaaggcg ttgaaggggt gacctctgtc agcgacaaac
     5521 tgcacgttcg cgacgctaaa gaaggctctg tgaagggcta cgcgggtgac accgccacca
     5581 ccagtgaaat caaagccaaa ctgctggcgg acgatatcgt cccttcccgt catgtgaaag
     5641 ttgaaaccac cgacggcgtg gttcagctct ccggtaccgt cgattcccag gcacagagcg
     5701 accgtgctga aagtatcgcc aaagcggtag atggtgtgaa aagcgttaaa aatgatctga
     5761 aaactaagta attcgtcgta attcgtcctc ccgaactttg tcgggaggcg cgatgtgcac
     5821 cacactaaaa atatcgcatt gagtgatttt cacgctcata tcaagcggtg acattaacta
     5881 tggtaaagga gacgcttatg tttcgttggg gcatcatatt tctggttatc gcgttaatcg
     5941 ccgccgcact tgggtttggt ggtctggccg gtaccgctgc aggcgcagct aaaattgtct
     6001 ttgtcgtcgg gattattctg ttcctggtga gtttgttcat gggccgaaaa cgaccctaga
     6061 tttcagacca ttaagaaaat tttatccaaa gccagtccag cggactggct tttgcggttt
     6121 tagcgaataa taaattgcgt tactttgtca ttctttcaca acaaaaacag gaaggcagag
     6181 gtggggcagc gaatacctgt aacgcttggt aatattgcgc cgttgtcgct aaggccgttc
     6241 cagcctggac gaatagctct ggtgtgcgaa ggcggcggac agcgtggaat tttcacggct
     6301 ggcgtgctgg atgagtttat gcgcgcgcag tttaatcctt tcgatcttta tctcggcaca
     6361 tctgccgggg cgcagaacct ctcggcgttt atctgcaatc agcccggtta cgcgcgcaaa
     6421 gtcatcatgc gctataccac aaaacgcgaa tttttcgatc cattgcgctt tgtccgtgga
     6481 ggaaatctta tcgatctcga ctggctggtg gaggccaccg caagccagat gccgttgcaa
     6541 atggataccg ccgcgcggtt gtttgacagc ggcaaatcgt tttatatgtg cgcctgtcgt
     6601 caggatgact acgcgcctaa ttacttttta ccaaccaaac aaaactggct ggatgtgatt
     6661 cgcgcctcca gtgcgatacc tggcttttat cgtagcggag tgtcgctgga aggcattaac
     6721 tacctggatg gcgggatcag tgatgcgatt ccggttaaag aggcggcaag gcagggcgct
     6781 aaaacgttgg tcgtcatccg cactgtgccg tcacaaatgt actacacgcc gcagtggttc
     6841 aaacgcatgg aacgctggct gggtgacagt agcctgcagc cgctggtcaa tctggtgcag
     6901 catcatgaaa ccagctatcg tgacattcag caatttattg agaaaccacc gggcaagctg
     6961 cggatattcg aaatttatcc accgaagcca ttacatagta tcgcgcttgg cagtcggatt
     7021 ccggcgctgc gtgaagacta taaacttggg cgtttatgcg gtcgttattt cctcgccacg
     7081 gttggcaagc tattaactga aaaagcgccg cttacccgcc atctggtgcc agtggtgacg
     7141 ccggaatcga tcgttattcc gcctgcgcca gtcgccaacg atacgctggt tgccgaagtg
     7201 agcgatgctc cgcaggcgaa cgacccgaca tttaacaatg aggatctggc ttgatttgcc
     7261 gttttatcga tacccactgc cattttgatt tcccgccgtt tagtggcgat gaagaggcca
     7321 gcctgcaacg cgcggcacaa gcgggcgtag gcaagatcat tgttccggcg acagaggcgg
     7381 aaaattttgc ccgcgtgcag gcgttagcgg aaaaatatca accgctgtat gccgcattgg
     7441 ggttgcatcc ggggatgttg gaaaaacata gcgatgtgtc tcttgatcag ctacagcagg
     7501 cgctggaaag gcgtccggcg aaggtggtgg cggtggggga gatcggtctg gatctcttcg
     7561 gcgacgatcc gcaatttgag aggcaacagt ggttactcga cgaacagctg aaactggcga
     7621 aacgctacga tctgccggtg atcctgcatt cacggcgcac acacgacaaa ctggcgatgc
     7681 atcttaaacg ccacgattta tcgcgcactg gcgtggttca cggtttttcc ggcagcctgc
     7741 aacaggccga gcggtttgta cagctgggct acaaaatcgg cgtaggcggt actatcacct
     7801 atccacgcgc cagtaaaacc cgcgatgtca tcgcaaaatt accgctggca tcgttgttgc
     7861 tggaaaccga cgcgccggat atgccgctca acggctttca ggggaagcct aaccgcccgg
     7921 aacaggctgc gcgggtgttt gacgtgcttt gcgagttacg tccggaacca gaagatgaga
     7981 ttgccgaagt gttgcttaat aatacgtatg cggtgtttag cgttcgtggg tagtttaccc
     8041 ttcgtaggca gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta
     8101 tcatgcctac aggtttgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc
     8161 cggaaatatc aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgcc
     8221 ttccggcgtg gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc
     8281 attcaggcga accggaacat cgccaagtct cttgataaac gtcgccagct catcgatgtg
     8341 atgcaaataa tccacctggt ctggaattac cagcaaacgc agttccgcca gcttaccgcg
     8401 ctctgccagc aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaattgctg
     8461 atgacattcg ctcccccacg ctttgagatc gagcatcgcg ccgtcgcaca ccgggagtaa
     8521 tttttcccag ccggtttcgc tcaacatacc gttactgtcc acgaggcagg tgaggtggcg
     8581 cagttgcgga tcgtttttga tggctgtaaa tagcgccact acaaacggca gttgggtcgt
     8641 ggcttcgccg ccgcttaccg ttatcccttc aataaacagc accgctttgc ggatatggct
     8701 aagcacctca tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacctctt
     8761 caggcaggta tcacactgct cgcaaaccac agcgttccac accactttgc cgtcaacaat
     8821 ctgcaacgcc tgatgcggac actgtggcac gcactcccca cagtcattgc aacgtcccat
     8881 cgtccacgga ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag
     8941 acgactgcct ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct
     9001 gctgttcatg gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt
     9061 cttcgcccag ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaagtccg
     9121 acaaacgcac catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcggtaa
     9181 attcacgcat tccggcttta aaggcaccga ggcaaagctg taccagtgcc tgcgggttac
     9241 gtttgatggt ttcgtcgagc gtcagaatgt cgctgatgcc ggaataataa taagcatgat
     9301 gcggggcgac agtttgcaga tgggtgatcg gatctggctc atcgccatac ggcaaacgcg
     9361 cgcccggcgt ggtgccgata tcggaactga tccccgactg tgcgtgtaac atggcgcgtt
     9421 tttgccagcc atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc
     9481 gataacccac ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct
     9541 cacacagcaa gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt
     9601 cagggttaat cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat
     9661 agaggaattc acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag
     9721 tgcgcgtaaa gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc
     9781 gtaccagcgt gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt
     9841 agcccccttt tgtgaaaaat ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac
     9901 attcacagat gttcttcgcc actttcagca gcaggtcatc aggggtgatt tcaggatcgt
     9961 agataaaggt caggttcggt gaaacctgct tcaaatctgc gtctgcacgt aagatcgcgt
    10021 gggtaatggg cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc
    10081 tgtcgaggta acgccagaaa cgttttattc gaacatcgat ctcgtcttgt gttagaattc
    10141 taacatatgg ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg
    10201 acggtacgtg gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc
    10261 cttccagctc cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat
    10321 agcgcggttt gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg
    10381 cgcggcgggc ttcggcagga agctgcggat aaggcagatt gttttctgct tccagagcca
    10441 gaaaatggcg cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt
    10501 gttgcagtgc attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg
    10561 cgccattgta agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac
    10621 gcattcattt gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa
    10681 gtagatttcc ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact
    10741 aacaaactcg caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg
    10801 aaagcaagca gcctgcgtgc actgaaattg atggacctga ccaccctgaa tgacgacgac
    10861 accgacgaga aagtaattgc tctgtgtcat caggccaaaa ctccggtcgg caataccgcc
    10921 gctatctgta tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc
    10981 accccggaaa tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa
    11041 atcgcgctgg cagaaacccg tgcggcaatc gcctacggtg ctgatgaagt tgacgttgtg
    11101 ttcccgtacc gcgcgctgat ggcaggtaac gagcaggttg gttttgacct ggtgaaagcc
    11161 tgtaaagagg cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa
    11221 ctgaaagacg aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac
    11281 ttcatcaaaa cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc
    11341 atgatggaag tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accagcgggc
    11401 ggcgtgcgta ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgttcggt
    11461 gctgactggg cagatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg
    11521 ttgaaagcgc tgggccacgg tgacggtaag agcgccagca gctactaagt aagatgcttt
    11581 acgcctgatg cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag
    11641 gcgtagacgc atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt
    11701 ctcgcacaag aaattattcg taaaaaacgt gatggtcatg cgctgagcga tgaagaaatt
    11761 cgtttcttta tcaacggcat tcgcgacaac actatctccg aagggcagat tgccgccctc
    11821 gcgatgacca ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg
    11881 atgcgagatt caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt
    11941 gataaacact ccaccggcgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc
    12001 gcagcctgcg gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt
    12061 acgctcgaca aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc
    12121 cgcgaaatta ttaaagacgt cggcgtagcg attatcggcc agaccagctc actggctccg
    12181 gcggataaac gtttctacgc gacccgtgat attaccgcaa ccgtggactc catcccgctg
    12241 atcactgcct cgatcctggc gaagaaactg gcggaaggtc tggacgcgct ggtgatggac
    12301 gtgaaagtgg gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa
    12361 gcgattgttg gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgatatg
    12421 aatcaggtac tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc
    12481 ctgacgggtg aatatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag
    12541 atgctgatct ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaggcg
    12601 gtgctggaca acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc
    12661 ccgaccgact tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca
    12721 gtctatgctg ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca
    12781 gtggttgcaa tgggcggcgg tcgtcgtcag gcatctgaca ccattgatta cagcgtcggc
    12841 tttactgata tggcgcgttt gggcgaccag gtagacggtc agcgtccgct ggcggttatc
    12901 cacgctaaag acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa
    12961 cttgccgata aagcaccgga aagcacacca actgtctatc gccgtatcag cgaataacgg
    13021 tatactgatc tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca
    13081 tttattatgg tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt
    13141 gacgtcgggg ctgacaccct gggtcatatc gcagaagctt gtgccaaagg cgaagctgat
    13201 aacggtcgta aaggcccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca
    13261 cacgaaggtt ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc
    13321 gcgtacgcat gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa
    13381 atcgccggtg tcccggttct gtttgagtgg ggatatttct ccgatcacga aaacagcttc
    13441 ccgcaagagc tgctggataa actggtcgaa cgcgctaatc tgccgggtta cctcggtaac
    13501 tgccactctt ccggtacggt cattctggat caactgggcg aagagcacat gaaaaccggc
    13561 aagccgattt tctatacctc cgctgactcc gtgttccaga ttgcctgcca tgaagaaact
    13621 ttcggtctgg ataaactcta tgaactgtgc gaaatcgccc gtgaagagct gaccaacggc
    13681 ggctacaaca tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc
    13741 cagcgtaccg gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag
    13801 aaactggttg atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc agacatctac
    13861 gccaactgcg gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc
    13921 accatcaaag agatgaaaga agcgggtgat aacaccatcg tcttcaccaa cttcgttgac
    13981 ttcgactctt cctggggcca ccgtcgcgac gtcgccggtt atgctgcggg tctggagctg
    14041 ttcgaccgcc gtctgccgga gctgatgtct ttgctgcgcg atgacgacat cctgatcctc
    14101 actgctgacc acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt
    14161 ccggtactgg tttacggccc gaaagtaaaa ccgggttcac tgggtcaccg tgaaaccttc
    14221 gcggatatcg gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa
    14281 gccatgttct gataatgggt gcggcttgat gccctcaccc cggccctctc ccacagggag
    14341 agggggaaaa catgttgata aggataaaac aatggctacc ccacacatta atgcagaaat
    14401 gggcgatttc gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcga agtatattgc
    14461 tgaaactttc cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac
    14521 cggtacttac aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg
    14581 ctccatctac accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg
    14641 ttcctgtggc gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc
    14701 ctgcaccgat tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc
    14761 tgactttgac atggtacgta acgcggtaga cgcggctaaa gcactgggca ttgatgctcg
    14821 cgttggtaac ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt
    14881 gatggaaaaa tacggcatcc tcggcgtgga aatggaagca gctggtatct acggcgtcgc
    14941 tgcagaattt ggcgcgaaag ccctgaccat ctgcaccgtg tctgaccaca tccgcactca
    15001 cgagcagacc actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact
    15061 ggaatccgtt ctgctgggcg ataaagagta attgtgtttc gctgaaaagc gattgtcgtg
    15121 tgaagccgga gtgggagact gctccggctt tttagtatct attcatcaa
//
LOCUS       DAZGTR010000103        15140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGTR010000103 DAZGTR010000000
VERSION     DAZGTR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.104"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..267
                     /locus_tag="ACHLCY_003604"
     CDS             <1..267
                     /locus_tag="ACHLCY_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0370782.1"
                     /translation="ATNNLKLGTFGAFDNEWHMLAFRFAGNNSLQVTPVIDGQDGTPF
                     TLTQSPVSAFAADKLHVTDITRNATYPVLIDSIAVEVNNADAAA"
     gene            404..583
                     /locus_tag="ACHLCY_003605"
     CDS             404..583
                     /locus_tag="ACHLCY_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0370783.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            624..869
                     /locus_tag="ACHLCY_003606"
     CDS             624..869
                     /locus_tag="ACHLCY_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0370784.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            947..1162
                     /locus_tag="ACHLCY_003607"
     CDS             947..1162
                     /locus_tag="ACHLCY_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0370785.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1167..1700
                     /locus_tag="ACHLCY_003608"
     CDS             1167..1700
                     /locus_tag="ACHLCY_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0370786.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATQVDGKLVVPGMKLSKEKCDQVNAIERDKALAWVAKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1974..2543
                     /locus_tag="ACHLCY_003609"
     CDS             1974..2543
                     /locus_tag="ACHLCY_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP0370787.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRDKVIQYQEEC
                     DDVLYEYWTKGGVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            2543..2692
                     /locus_tag="ACHLCY_003610"
     CDS             2543..2692
                     /locus_tag="ACHLCY_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370788.1"
                     /translation="MEWWLMISDKLITLAKGLCVIVGISFSLMLVVLFLSINWMALTS
                     AGLVG"
     gene            2695..3132
                     /locus_tag="ACHLCY_003611"
     CDS             2695..3132
                     /locus_tag="ACHLCY_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0370789.1"
                     /translation="MNRMLSAFTVILLVVCGALSLGLNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            3335..3886
                     /locus_tag="ACHLCY_003612"
     CDS             3335..3886
                     /locus_tag="ACHLCY_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0370790.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            4179..4985
                     /locus_tag="ACHLCY_003613"
     CDS             4179..4985
                     /locus_tag="ACHLCY_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0370791.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            4966..6672
                     /locus_tag="ACHLCY_003614"
     CDS             4966..6672
                     /locus_tag="ACHLCY_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0370792.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            6672..8816
                     /locus_tag="ACHLCY_003615"
     CDS             6672..8816
                     /locus_tag="ACHLCY_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0370793.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRQQNEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNDLMEQI
                     ERPDGIIKLNPVRKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            8974..9981
                     /locus_tag="ACHLCY_003616"
     CDS             8974..9981
                     /locus_tag="ACHLCY_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370794.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNKADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            10005..11219
                     /locus_tag="ACHLCY_003617"
     CDS             10005..11219
                     /locus_tag="ACHLCY_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP0370795.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            11275..11664
                     /locus_tag="ACHLCY_003618"
     CDS             11275..11664
                     /locus_tag="ACHLCY_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370796.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            11714..12175
                     /locus_tag="ACHLCY_003619"
     CDS             11714..12175
                     /locus_tag="ACHLCY_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370797.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            12159..12722
                     /locus_tag="ACHLCY_003620"
     CDS             12159..12722
                     /locus_tag="ACHLCY_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370798.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFF"
     gene            12722..13372
                     /locus_tag="ACHLCY_003621"
     CDS             12722..13372
                     /locus_tag="ACHLCY_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP0370799.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPAPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVEWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            13369..>15140
                     /locus_tag="ACHLCY_003622"
     CDS             13369..>15140
                     /locus_tag="ACHLCY_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0370800.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAVTAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGERGPQGIPGLKGDTGERGPKGDQGDMGPKGEKG
                     DPGGPAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGPAGPRGERGETGPQGPRG
                     EPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDVANEAKTKAEE
                     AAAGGGVPGPKGDKGDTGPAGPAGPKGDKGERGDTGPVGATGERGPAGDAGPAGPQGP
                     KGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETGAAGPVGATGPQGPKGDP
                     GETQIRFRLGPGNIIETNSHGWFP"
BASE COUNT         3910 a         3482 c         4433 g         3315 t
ORIGIN      
        1 gcgacaaaca acctgaaact gggaaccttt ggcgcatttg ataacgaatg gcatatgctg
       61 gctttccgct ttgccgggaa taacagcctt caggtgacgc cggttattga tggtcaggat
      121 ggcacaccgt tcacgctgac gcagtcaccg gtcagtgcat ttgcggcgga taaactgcat
      181 gtgacagaca ttaccaggaa tgcgacttac ccggtgctga ttgacagcat tgcggtggaa
      241 gtgaacaacg cggatgccgc ggcatgataa aaaaagccgc cagttgcctg agacctctgg
      301 cggtgtgaga ttcatggaga atcaaggaaa gatactacca ctttcgtcat cggcattttt
      361 aaatgaaaac tgtttactta gtcaaccata acggtaagaa attatgacat ttctgaacca
      421 gttaatgctg tacttctgta cggtggtctg tgtgctgtat ctcctttcgg gtgggtacag
      481 ggccatgcgt gacttctggc gcagacagat tgacaaaagg gccgctgaga aaatcagcgc
      541 cagtcagtca gccggaagca aacccgaaga gccgctcatt tagcggcaac tttcttaatc
      601 acatctttcg acgagaaaat cccatgtcag aaattacatc cctggtcact gctgaagcag
      661 tgaaggaagt cctgcgctct gaagaagtcc ggagcgcact gaaacagaaa cttcgccata
      721 acctggaagc gcgtcttgat gcagaagtgg atgccattct ggatgaactg ctgggcgcac
      781 cggcagctcc ggagccggaa ggcatcgcgg gtgaggggag tgcttcagat agcggtgacc
      841 ccacaccgga cagcgacatg atgatgtaag catgcgtcag ggaccatcgg tgtgtgccgg
      901 tggtcttttt tattgttgtg agcttccgga ttgcgggagg cggggtatga accagatgga
      961 aaaaatcaca acaggtgtgt catacaccac gtcagcggtg ggaacgggct actggttcct
     1021 gcagttgctg gacagggttt ccccgtctca gtgggcggca ataggcgtgc tggggagtct
     1081 gctgtttggt ctgctgacgt acctgacgaa cctgtatttc aaaatcagag aggacaggcg
     1141 taaggcggca cggggagagt aagctgatga gcaggaaact ccgctatggt ttatcggctg
     1201 ccgttctggc gctgattgcc gcaggtgctt ctgcgcctga aatcctcgac cagtttctgg
     1261 atgaaaagga aggcaaccac accacggcat accgtgatgg tgtgggtatc tggaccatct
     1321 gccgtggtgc cacccaggtg gatggtaagc ttgtcgtccc cggcatgaag ttgtcgaagg
     1381 aaaaatgcga ccaggttaac gccattgaac gtgataaggc gctggcatgg gtggcgaaaa
     1441 acatcagagt gccactgacc gaacctcaga aagcggggat cgcgtcattc tgtccttaca
     1501 acattggccc cggtaagtgt ttcccgtcga cgttttataa acgaattaat gcaggcgatc
     1561 gcaggggagc gtgtgaagcg attcgctggt ggattaagga cggtggcaga gactgccgta
     1621 tccgctcaaa taattgctac ggtcaggtat cccgtcgcga ccaggagagt gcgctggcat
     1681 gctggggtat cgacaggtag cagaatattt tgctgaaaaa tgacgtttgc tcacgcggac
     1741 ggataacacg aaatcctgcg aactggcaaa atgcaagtga ataaagtcag gaagattgtt
     1801 tcacgctgag gcaccgtaat ggtgtctttg tcatttctgc gcttcgcaca agcgtaaata
     1861 aaccaaagaa cctttcagga tgagccctgg tggataaccg gcagtggtct ggttaaccct
     1921 ctttgggctg gttattcctg tgcgcagggt tcatcactaa aaggaaataa ccgatgaata
     1981 tgatgaccgt gccgtttcac ggcgattctc tttatgtggt taaccataac ggtgagccat
     2041 acgttcccat gaaacctgtc gttgcgggga tggggctggc ctggcaatca cagttggcta
     2101 agttaagaca gcgttttgcg tcaactataa cggaaatcgt tatggttgct gaggatggga
     2161 aacgacgcaa tatggtgtcc ctgccacttc gaaaacttgc aggctggtta caaaccatca
     2221 atcccaacaa agtaaaaccc gaaatccgcg acaaggtaat ccagtatcag gaagagtgcg
     2281 acgatgttct ctatgaatac tggacgaagg ggggtgtcgt taatccccgt cgaatgagtg
     2341 tgatggaaga actcaatcag gcctgcgctg acatgaaacg ggataaaaac attgccagtg
     2401 tgtttgctac cgggctgaat gagtggaaac aggttaaagc cgcgcatgta tcaaaaatcc
     2461 gcacattgat aaacgaagcg aatctgctga ttgattttgt cctggctgat acaggcaaag
     2521 ggaaaataac aaaggcggat tgatggagtg gtggctaatg atatcggata aactcataac
     2581 gctggcgaag ggcctttgtg taatcgtcgg tatttcattt tcactaatgc tggttgttct
     2641 ttttctttcc ataaactgga tggcgttgac ttcggcaggg ctggtggggt gagcataaac
     2701 cgaatgcttt ccgcgtttac cgttattctg ctggtggtct gtggtgcgct tagtctgggg
     2761 ctgaatcatt accgtgataa cgccatcgct tacaaagagc agcgcgataa agccacatcc
     2821 atcatcgcag atatgcagaa gcggcaacgt gatgtagcag aacttgacgc cagatacaca
     2881 aaggagcttg ctgatgctaa tgcgactatc gaaagtctcc gtgctgatgt ttctgctggg
     2941 cgtaagcgcc tgcaagtcgc cgccacctgt gcaaagtcaa cgaccggagc cagcagcatg
     3001 ggcgatggag aaagcccagg acttacagca gatgctgaac tcaattatta ccgtctcaga
     3061 agtggaatcg acaagataac tgcgcaggtt aactacctgc aggaatacat caggacgcaa
     3121 tgcctgaaat aatttttttg caaatcacaa agtcaattta atgagcctcg cgatacgggg
     3181 cttttttatg tccgcagtaa acgcgcttca cacgcgcgac ttatgaacac agaacctttc
     3241 aggatgaccc ttgaggatgc cggtttggtg atcggtgcct ttctgtgggc cggaatcctg
     3301 tgtgacaagg ttcatcacta aaaggtgtgc actgatgaat tatccaacta tcgttaacgg
     3361 catcgatttt cgagatcttg tttttgtgac aggcaacgag ccggtgacgg atacccgaaa
     3421 ggttgccgta gcatttggca aagaacacaa agatgtattg cggaaaacaa gagccgttgt
     3481 tcaacaatgc tcaaaagaat ttgcagagcg caattttgcg ctttgctatg aaaacaatga
     3541 gttacagaac ggtaaacccc agcccatata tcgaatgaca aaagacggtt ggacgatgtt
     3601 ggtatttggt tttacgggga aggctgctat gacctttaag gaagcgtaca ttcaggcttt
     3661 caactggatg gctgagctta ttcagcaagg tctggcgaat ctggaggcgg agcgcaacgc
     3721 tgtaatgctg gagtacatga aagagaaaga tgtcgccagt atgtcaggtc gtttgctcaa
     3781 tcgctggggc agggtgaaga agcctcagtt gctggcaagg ctggacaggc tggagcagca
     3841 ggggcagatt gcgttacccg gatttgataa aggcatttca gcctgacata gccatgcgcc
     3901 gtatcgtcgc cgtattcccg cattaacaga gactgcagcc accttatctg cgtgagtgtg
     3961 cggggataat caaaaacgat gcacaccggg ttttctcatt tttcacgaga tgggagcgat
     4021 ttcccgcgaa gccgcctgtc cggtgcggtg gtggaagaaa ccggataaaa caaccgcatt
     4081 gtgcaaatat cgatcaaata tggtgctgct gtgtgaaatc tgaaaaatca cagcggtcat
     4141 tatgcatcag tttttaacac aggacgtcag aaagtgacat ggcaaagctg gactggaaaa
     4201 agctggagca ggcattccga cgcgaacatg ccgaaacggg aataacatta ctggactggt
     4261 gccggaagaa aaagattaat tacaacaccg ccagaacccg tataaaaatg ggcaaaatcg
     4321 atcatgaaat tgatcataaa accgatcatg aaatcgatca tgacatctca gatgaagaac
     4381 cctgcaatga cgcgggttcc ggcgatgaaa aatgcgcaaa aaactctgaa aaaaactgcg
     4441 caaattcggc agaaacgaaa cggattcgtg gttcccgact tttaccacct tcaaacgctt
     4501 tttctcagcg aaacacccac gccgtaagac accgtggata tgcgaagtat cttgaggcag
     4561 ataacctcat ggatgatgcg tccgacatgg tgctgttcga tgaactggtg ttcacccggg
     4621 cccgcgcact ttcagtaact aaggcactta aagggatgtt cgccgacctg gaagaggcaa
     4681 ctgacgtgga aacccgcgtt gctctttacg acaaaatact caaagctgaa caggcccttg
     4741 accggaatat tgcccgtatc gagtcaattg aacgctcatt gctgacgctg gacgtcctgg
     4801 ctgagacagc accaaaactt cgtgctgacc gggaaagaat caacgccgcc agagataaac
     4861 tcagagcgga aaccgatatt ctgaccagcc agcgtcgggg cgttgttacg cctgtcagtg
     4921 acatcgtgtc atcgctgcat gaaatgagta attcggggag actggatgac attccggaag
     4981 aatgaaccgc gatgtgatga gccgtcagaa atgaccgagg ctgaacaacg tctgttcatc
     5041 atgactaaac tgagcaatcc ctggtggcgg ctcaatcatc tctacaaaat acagaacgaa
     5101 aaaggtgaac tggtcacctt cagaatgcga ccggcgcagc gccagttgtt ccggagcatg
     5161 cacaataaaa atattatcct gaaagcgcgc cagctgggat tttccacagc cattgatatt
     5221 tatcttctcg accaggcatt attcattccg catctcaaat gcgggatcgt cgctcaggat
     5281 aaacaggctg ccagtgaaat tttccgcaca aaaattgctg taccgtttga tcatctccct
     5341 gactggctga gagcctcatt caccatcgtt gaacgtcgta gcggtgccag cggtggctat
     5401 atcctgtttg gtcacggctc gagtatccag gtggcaacct cattccgttc aggtacggtg
     5461 cagcgcctgc atatctcaga gcacggcaaa atttgcgcga aatatccggc taaggcgaaa
     5521 gaactgcgaa ccggtacgct taatgccgtc tctgatgaat gcattatttt tgatgagtcc
     5581 actgctgaag gcgtgggtgg tgatttttac gagatgagta accgagcaca ggagatcact
     5641 gcatcaggct tattgctgac ggcacaggat tataaattcc atttttacgc ctggtggcag
     5701 gatcctaaat acagcgccag agtgccggaa agcgggctga agctgtcacg ggaaaaaatg
     5761 acgtattttt ctgcggttga gaaggcaatg aacatcacgc ttactgatga acagaagcag
     5821 tggtacatca ataaggaaac tgaacagcgt gaggaaatga agcaggagtt tccctcaacg
     5881 ccacaggagg cgtttctgac gtccggacga cgtgtgttca gtgccgaaag tacgttgcag
     5941 gcagaatcat tctgttcgcc accgatgatt gtttatgaca ttgaacctgt tacaggagcg
     6001 aagactaaag ctcagtctct gcgtgaagga aataaaaacg agttgcagcg gacgctgatg
     6061 aattatctgc tggtatggga actgccggat ccggatgaag agtatgtttg tggggcagat
     6121 actgccgaag ggctggagca cggagaccgc tcatcgctgg atgttgtcaa acgcagtaat
     6181 ggcgagcagg tggctcactg gttcgggcat ctcgatgctg aactttttgc tcatctcatt
     6241 tcgcaggtct gtcgtatgta taacaacgcg tttgtggggc cggagcgtaa taatcacgga
     6301 catgcagtta tcctgaaact ccgggaactc tatccgacac gttatatcta caacgaacag
     6361 catcttgacc aggcatatga cgacgatacg ccccgccttg gctggctgac aacccgtcag
     6421 agcaaacctg ttctgaccga aggaatgaaa acgcttctga ataatggaat atcagggatc
     6481 cgctggtcag gcacattatc ggaaatgaac acctacgttt atgacgcgaa aggctccatg
     6541 aatgcacagg aaggctgctt tgatgatcag ctcatgagct acatgattgc ccaggagatg
     6601 cgcgccagaa tgccggtgag ggtaaaacag aaaacggata aacgcagaac cacacactgg
     6661 atggctcact gatgaaaaat gaaactaaca ccatggcgac gaaaaacgac aatggagcca
     6721 cgccgcgttt ttctcagcgc cagttacagg cgctttgttc tgatattgac agccagccta
     6781 aatggcgtga tgccgcaaac aaggcctgtg cgtattacga tggcgatcag ttgccaccgg
     6841 aagttcttca ggtactgaaa gatcgcggtc agccgatgac tatccataac ctcatcgcgc
     6901 ctaccgtcga tggcgttctg ggaatggagg ccaaaacacg gactgatctg gtggtgatgt
     6961 cagacgagcc agatgatgaa actgaaaaac tggctgaagc tattaatgct gaatttgccg
     7021 atgcatgccg ccttggcaat atgaataaag cccgctctga tgcctatgcg gaacaaatca
     7081 aggcgggcct cagttgggtg gaggtcagac gaaacagcga tccgttcggg cctgaattta
     7141 aggtgtctac tgtcagccgg aatgaggttt tctgggactg gctgagccgg gaggctgatt
     7201 taagtgactg ccgatggctg atgcgtcgcc gctggatgga taccgatgag gcaaaagcta
     7261 cattcccggg aatggctcag gttatcgatt atgccattga tgactggcgt ggttttgtcg
     7321 ataccacggt tactgaaggc cagcccagtc cgttgatgag tgcatgggaa gagtatcagt
     7381 catgggatcg acagcagaac gaatggcttc agcgtgaacg ccgtcgtgtg ctgcttcagg
     7441 tggtttatta ccgtacattc gagcgtcttc cggtgattga actcagtaat ggacgggtgg
     7501 tggcctttga taaaaataat ctgatgcagg cggtagctgt ggcatccggg cgggttcagg
     7561 tgaaagtcgg gcgggtaagc cgtattcgtg aagcctggtt tgtcgggcca cactttattg
     7621 tggatcgccc ctgtagtgct ccgcagggga tgtttccgct ggttcctttc tggggatacc
     7681 gaaaggataa aaccggggag ccatacgggc taatttcccg cgccattccg gcacaggatg
     7741 aggtgaattt tcgtcgtatc aagctgacct ggttgcttca ggccaaacgc gtgattatgg
     7801 acgaggatgc cacccagttg tcagacaacg acctgatgga gcagatcgaa cgtccggatg
     7861 gcattattaa actgaatccg gtccgaaaaa atcagaaaag tgtcgcagat gtttttcggg
     7921 ttgagcagga ttttcaggtt gccagccagc agtttcaggt catgcaggaa tcggaaaaac
     7981 ttatccagga taccatggga gtgtattccg catttctcgg gcaggattca ggtgcgacgt
     8041 caggcgtggc tatcagtaac ctggtggagc agggggccac aacccttgcg gaaatcaacg
     8101 ataactacca gtttgcctgc cagcaggtgg gaagactgtt gctggcttat cttctcgatg
     8161 acctgaaaaa acgccgtaat catgcagtgg tgattaatcg cgatgatcgc cagcgtcgcc
     8221 agaccattgt cctcaatgct gaaggtgata atggtgaact gaccaatgat atttcaaggt
     8281 taaatacaca tattgcgctg gcgcctgttc agcagacacc ggcgtttaag gcacagcttg
     8341 cacagagaat gtcagaggtt attcaggggc tgccgcctca ggtgcaggct gttgtgctcg
     8401 acctgtgggt taatcttctg gatgtgccgc agaaacagga gtttgttgag cgtattcgtg
     8461 ctgcgctggg gacgccaaaa tcaccggatg aaatgacgcc ggaagaacag gaagtagcgg
     8521 cacaacaaca ggcacttcag caacaacagg cagaactcca gatgcgcgag atggctggca
     8581 gagtggcaaa actggaagct gacgccgcca gggcacatgc agctgcacaa cgggataatg
     8641 ccagtgcaca gcgggaagtc gccctgacac aggggcagcg ttatgtggat gcgcttaacc
     8701 aggcacatac ggcagaaatc attaccggcg tacagaatat ggaacaggag caggacgttc
     8761 ttcagcaaca gatgctgtat acgttacaac agcggatgaa tgaaatgtcg ctctgaaaac
     8821 tctggcttca actgaacccc gtcatcgtac ggggtttttt gtttccggag gtaagcgttc
     8881 cgggagcggt gcgcttattc gcgggggcag cgataagcct tatttactca accattcgga
     8941 tctgtccgat aaacagacca tgcggagtta tttatggatt ttgaatttac gggtgaagaa
     9001 accccggaac aactggaaaa aatgctggaa ggacttgggg atgtggatat tgacagtcac
     9061 gcacaggacg tcgtgacgga agataccacg gaaaaacatg cggatgagga agcacagact
     9121 cagacgggcg ataacaatgt ggcaccgacg ccggatgcca gtgtggagca gacgcaggac
     9181 gtgaaggagc cggaagcgaa gggggtgctc acccgcgacg gtaaacacgt cattccctat
     9241 gaagtccttg aggctgaacg ttccggtaag caacgggccg aacaggaagc cgcacttctt
     9301 cgtgggcaga tagctgaaga aaaacgcagg gtggaactgc tgacgtctca gatccaccag
     9361 gccggtatga agcccacacc gttaccggaa aacgaaaaaa tttctgatga gcagattgcc
     9421 cgtatcaggg agatgtatcc ggaaattggt gacgcggtgg cttcgctcat ccgtaaaaat
     9481 aactatctcc agtcccgtgt tcagcaatca gcacagcagg cagaaggtaa tggtggtgag
     9541 gatttatcac cggttcttga tgcgatgaat gccgtgccgg tgctgaaaac gtggcaggag
     9601 tccgatccag atcgcttctc ggttgctgta tccatcgacg ggaagctcca gaatgacccc
     9661 gcatggaaag acaaaacgct cactgaacgt ttcgctgaag tggcccgtcg tacgcaggtt
     9721 gctttcggtg aagtcagtga gtcgtctgct gacaacaagg cagacaaaac ggatatccgg
     9781 aaaacggcgg aagagaaagt gaagaccgct gaacaggagc aggcagtacc tgcttccccg
     9841 tcagatttag gcaccacggc ttccgtcgga accggtgata attttgaacg gttacttggc
     9901 gcttctcatt cagaggcaga ggcgattatg cgcggtatga cgaatgctga aatagacgcg
     9961 cttctggaga agctcgggta acttactgaa ggagaactga agtaatgacg actgtaacat
    10021 cagcccaggc gaataagctg tatcaggtgg cgctttttac cgctgccaac cgcaaccgct
    10081 cgatggtcaa tatcctcact gaacagcagg aagcgccaaa agcggtttcg ccggacaaga
    10141 aaagcacgaa gcagaccagc gcgggtgcgc cggttgtccg tatcacagac cttaacaaac
    10201 aggccggtga tgaagtgacc ttcagcatca tgcacaaact ctcaaaacgc ccgacgatgg
    10261 gagatgagcg tgttgaaggt cgtggtgagg atctcagcca tgctgacttc tccctgaaaa
    10321 tcaatcaggg acgtcacctg gtggatgcag gcggacgtat gagtcagcag cgcacgaagt
    10381 ttaacttggc atcctcagcc agaacgcttc tggggacgta ctttaatgac ctgcaggacc
    10441 agtgtgcgat agtgcatctt gctggagctc gtggtgattt tgttgctgac gacactattc
    10501 tgccgacagc ggagcaccct gaattcaaaa aaatcatgat caacgatgta ctgcctccga
    10561 cacatgaccg tcactttttt ggcggtgatg cgacaagctt tgagcagatt gaagcggcag
    10621 atattttttc tattggcctg gtggacaatc tctccctgtt cattgacgaa atggcgcatc
    10681 cgttacagcc ggttcgtctg tccggtgatg aacttcacgg agaagatcca tattacgtcc
    10741 tgtacgtcac gccgcgtcag tggaatgact ggtacacctc gacgtccggt aaggactgga
    10801 accagatgat ggttcgtgcc gtgaaccgtg caaaaggttt taatcatccg ctgttcaaag
    10861 gtgaatgtgc gatgtggcgc aatatcctgg ttcgtaagta tgcgggtatg ccgatccgtt
    10921 tctatcaggg gtcaaaggtt ctggtatcag agaataacct gacggcaacc acgaaagagg
    10981 tcgctgctgc aaccaatatt gaccgcgcca tgttactggg ggctcaggcg ctggcaaatg
    11041 cttacggtca gaaggcgggc ggtcacttca acatggttga gaagaaaacg gatatggata
    11101 accgtactga gatagcaatc agctggatca acggtctgaa aaaaatccgt ttccccgaga
    11161 agagcggcaa gatgcaggat cacggcgtga ttgccgttga tacagcagtg aagctctgat
    11221 tttttccttt ccctatgccg ggttttcgcc cggctttttc aggagtcatt aattatggca
    11281 aagactatcc ttgccccgtc actgagtgaa cgggtctata cgggtacgca cggtaatgag
    11341 tcggtggcag aaggcgtatt tacggtgaat gctgcggaag cggacagtgt tattcatctt
    11401 ctctcactgc cagtgggcat ccgtatcaac tcactccaac tggtttcaac gggtggtctg
    11461 ggtactgcaa ccgtcagcat taagtccggt gagcatgctc tcatcgataa cagcgaagct
    11521 gtttctgcaa aatttgccag atatgtgcca gtggagccgt acaccacaca gcgtgacggg
    11581 gagctggtta ctgtcaccat taagactgcc gctgcaaccg gcaccctgaa tgttctgctg
    11641 cgttataccg tggtgggata ctgattaaaa ccttccggcc cgcgtcatgc gggcttttta
    11701 tccggggaat tatatgagtg agaaaattgc cgttgtctat atcggcccaa aacccgtgaa
    11761 aaaggacacc attaccggaa gccgcacatt gttcccacgt cttgagccgg tgcatgttga
    11821 cagtgcgatg gcctggcaac tgctggggtt tccggatgtc tgggttcgtc atgaagagct
    11881 tgatgatgtt ctgaaaaagc aacaacagaa tgagcagttg cggcaggcac agcaggcgca
    11941 ggaaagagtg cttgctgcgc gggcagaagc ggagaacagt tttgttgttt ctgttaacgg
    12001 gcaggaggtg gatttaagta agctcacctc agcacggctg gcgacgctgt gtgaggcaga
    12061 agagctggat attcacaaag acccgaaaga aacggctgag gcattccgta tccgggtgcg
    12121 tgaggcattt cgccgtcgtg ttgcggagac tgaacagcat ggcggaactg agtgattttt
    12181 taccgtatgt ccgtcgtcat atcagcggtc cactgaacat tatgatgacg gatgctctgt
    12241 caatggctgc cgtggcattc agccgccagt cgttggtgtg ccgtcgggag gttactgttg
    12301 taccagtagc aggaaaagaa atcgtgcttc cgtatgacaa agatgatgag gagtgcgttc
    12361 atatcatccg tatctctgac gataatcatg agctttttgt cggtcgggat gtggatatca
    12421 gctccggacg ctccctgcga tttgcctgtt ctcccggtga ggtgagcgtg ctttatgccg
    12481 tcgctccgaa agccggacgc agccagatac cggatgaact cctcacatgg cctgaagaag
    12541 tggctgcggg ggcacttgag cggttgttca tgcagactgg tgtttcatgg tcagatccgt
    12601 tacgcgcaca gtatttttct gtgcagtttt ctgaggggat ccgtcgggca tatcgtcata
    12661 cactggcgac aagcccgtac tcttcatacc gcaaccctgt acgcaggcag aggtttttct
    12721 gatgacgacg attacagaaa tcatcggacg tgtgaataca caactggttg acccgatgat
    12781 ggttcgctgg cccctgcagg aattgtgcga ttattacaat gatgctgtga gggcagtgat
    12841 tctggcgaga ccggatgctg gcgcaagcct ggaaacaata agttgtgttc ctggcgcccg
    12901 tcaggttttg cccgatggtg taatacaact tcttgacgtg atatgcctca gtgacggtag
    12961 tgcagtcaga ccattatccc gggaggtgct ggatgcgcag tatcctgagt ggcccacaat
    13021 gaagggtatt cctgaatgtt ttatcagcaa cgacctgtcc ccgcgcgtat tctggctgtt
    13081 tcctgctcct gacaaagaga taagtattga tgcagtggta agccggatac cggaggcagt
    13141 gtatgttctg acgcaggacg atgatacgcc agttccactg gaagaggctt atgttaaccc
    13201 actggtggag tggatgttgt ttcgcgcttt cagtaaggat gctgccggtg gcgcagaatc
    13261 ggggctggct gcgcagcatt atcagagttt tgttgagcaa cttgggatca aacagggggc
    13321 agacagtgca ttgtctgccc gtaaaaaagt gtttaacgga ggtggagtgt gagtgttgtt
    13381 gtttcgggga cgctgaaatc tcctgatggt gaggcgatat caggagcaaa tattaccctg
    13441 acggcgctga cagtttcacc ggatgcgctc agcggcacca gtgcgtcggc agtgacccgt
    13501 gaaggtggat attacggaat gacgatggat ccgggggagt atgcggtttc ggtgacggtg
    13561 aaagggaaga ctgctgtcta cggacgtgtg cgtattgagg ggaccgaaag tacggtgacg
    13621 ctcaatatgc tgttacgccg tagtcttgtt gaggttagca tacccggaga actgctgaca
    13681 gatttccggc agatacagaa taatgtggct gatgaccttg ccactattcg tcgcctgaat
    13741 gaagacacgg cgacaaaaaa cactcaggcc acacagtcaa aagaaagtgc agcagccagt
    13801 gcgaagagtg catctgacag tgcaaagacg gcaaccagca gggcggctga agccggacaa
    13861 aaagcgactg atgccactga ggctgcgacc cgtgcagtca cagcagcggg gaatgcagag
    13921 gaaagctcga cccgtgccgg agagtctgaa aaagccgccg gagctgatgc agaaaaagcc
    13981 agacagcatg ctgaaaaggc caggctggcg caggagagcg ccggagagat ccttaagcgg
    14041 gcagaggctg ccactgtcag tgctgaagag gccagacgta tggctgagaa tgcacggggg
    14101 ccccgggggc ctcagggaga aactggtccg aagggggatg tcggtcctaa aggcgaaaca
    14161 ggtccagtgg gccctcaagg gcccgcaggg ccgaaaggtg agcgtggtga cgttggtgct
    14221 cagggggctg tagggcctgc tggtccgcgt ggtgagaagg gcgaacaggg ggagcgagga
    14281 ccgcagggaa taccaggcct gaagggggat accggagagc gggggcctaa aggggaccag
    14341 ggggatatgg ggccaaaagg cgagaaaggt gatccgggag gtcctgcagg cccgcaaggt
    14401 cctaaaggcg aacgaggaga agccggacca cagggaccga tgggagcacg aggtgagcgt
    14461 ggggagactg gcccccgagg tgaacctggt cctgcaggtc cgagaggcga acgaggagag
    14521 accggacctc agggacctcg tggagagcca ggtccggcag gcagcgctgc aaatgtggct
    14581 gatgcaacga cggcacagaa gggaattgtg cagttaagca gcgcaacgga cagtgatgat
    14641 gaaacgaagg ctgccacccc gaaagcggtg aaagcggcaa tggatgtggc aaatgaagcg
    14701 aaaacaaagg cagaagaggc tgcagcagga ggtggtgttc ccggtccgaa aggagataaa
    14761 ggggacacgg ggccagcagg tccggctggg ccgaagggtg ataagggaga gcgcggtgac
    14821 accggccctg tcggggcaac cggcgaacgg ggaccggcag gtgatgctgg tccggcaggc
    14881 ccgcaggggc cgaaaggtga caggggagag cggggagaga ccggtctgac gggaaatgca
    14941 ggtccacagg gtccaaaggg agataccggt gcggcaggcc cggcaggccc acagggaccg
    15001 aaaggagaaa caggtgcggc aggcccggtg ggggcgaccg gacctcaggg gccgaagggc
    15061 gacccggggg agacgcaaat acggttccgt ctggggccgg gaaacattat tgagacaaac
    15121 agccatggct ggttcccgga
//
LOCUS       DAZGTR010000104        15089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGTR010000104 DAZGTR010000000
VERSION     DAZGTR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.105"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHLCY_003623"
     CDS             complement(37..273)
                     /gene="yjjZ"
                     /locus_tag="ACHLCY_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP0370801.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="ACHLCY_003624"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="ACHLCY_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP0370802.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHLCY_003625"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="ACHLCY_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP0370803.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHSY"
     gene            complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHLCY_003626"
     CDS             complement(1875..2600)
                     /gene="yjjQ"
                     /locus_tag="ACHLCY_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0370804.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHLCY_003627"
     CDS             3219..3989
                     /gene="yjjP"
                     /locus_tag="ACHLCY_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP0370805.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHLCY_003628"
     CDS             3980..4453
                     /gene="yjjB"
                     /locus_tag="ACHLCY_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP0370806.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHLCY_003629"
     CDS             4560..5099
                     /gene="dnaT"
                     /locus_tag="ACHLCY_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP0370807.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHLCY_003630"
     CDS             5102..5839
                     /gene="dnaC"
                     /locus_tag="ACHLCY_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP0370808.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHLCY_003631"
     CDS             5888..6382
                     /gene="yjjA"
                     /locus_tag="ACHLCY_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP0370809.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            6636..8927
                     /gene="opgB"
                     /locus_tag="ACHLCY_003632"
     CDS             6636..8927
                     /gene="opgB"
                     /locus_tag="ACHLCY_003632"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP0370810.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(9067..10089)
                     /gene="lgoD"
                     /locus_tag="ACHLCY_003633"
     CDS             complement(9067..10089)
                     /gene="lgoD"
                     /locus_tag="ACHLCY_003633"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP0370811.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            10228..11142
                     /gene="lgoR"
                     /locus_tag="ACHLCY_003634"
     CDS             10228..11142
                     /gene="lgoR"
                     /locus_tag="ACHLCY_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP0370812.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     APLALRNHLNSAKQSMIRSINENTRYAH"
     gene            11357..12718
                     /gene="lgoT"
                     /locus_tag="ACHLCY_003635"
     CDS             11357..12718
                     /gene="lgoT"
                     /locus_tag="ACHLCY_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0370813.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPYGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(12767..14431)
                     /gene="tsr"
                     /locus_tag="ACHLCY_003636"
     CDS             complement(12767..14431)
                     /gene="tsr"
                     /locus_tag="ACHLCY_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP0370814.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TKAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(14550..14996)
                     /gene="hpaR"
                     /locus_tag="ACHLCY_003637"
     CDS             complement(14550..14996)
                     /gene="hpaR"
                     /locus_tag="ACHLCY_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFP0370815.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         3768 a         3921 c         3568 g         3832 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccgacgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggt gataaatccg catacatcag ccctgcaatc agcaatcctg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagctag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatgtta tctggcgaac gcatctccag ccacaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccgaaca tcgtgaacat ttgctggagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaatggtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gccgtgccgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaagg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatcttac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg cttactaccg
     1081 gtgaagataa tccactctgg cgtaccgtgg tgctacgcga cggcctgctg gtgcgccgga
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taataggaat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gccagtcgac gcatcgcact gtttttttgg
     2041 gcgctgatag ttttattact tttcttaagc aacgaagcta tctggttgat gccccatcct
     2101 ttacctaata gccgcagtac cttgcgctct gaaagagtga gaatgacgga gcgatagcta
     2161 aaatcatgag tttcattcat tgccggagag gtcagcatgg cgctgatacg ctctgcgtgc
     2221 gtattgccgg aacgtacggt cttgaccaga ttctcaatgg gttcaacatc agacaataac
     2281 gtcgccgtag ggcacatgag caattctact gcctgggaat accaggagcg tgacaccatg
     2341 aaaacccagt gaatttcccg atattgtgag attaaagaat aataatgttc acaaacagaa
     2401 cgaggatctt cactttcacc ggctaaatca gcaatgacta atccggaacg acgtaattgt
     2461 aataaggtaa ggtcctccgc agaggcgctg gatattattt catattcagg gaagtgagtc
     2521 ctcatgacct cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt
     2581 ttgcagcatc ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta
     2641 ttcaggatat tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat
     2701 ccttagcatt cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt
     2761 cagaaggtta gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta
     2821 cattaagttg ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt
     2881 aaatgatatc ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc
     2941 tgatggtttt aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt
     3001 aaaggccaaa caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg
     3061 agagataatt cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat
     3121 tttgatctta acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc
     3181 tgaacaatac gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag
     3241 ccgtaacacg gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg
     3301 cgttggttga tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa
     3361 gttctatctc ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat
     3421 cgacacgtaa aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca
     3481 ttgtgattct ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc
     3541 aaattcagcc attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgtg
     3601 cctgtttctg taaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca
     3661 gtacggccgc gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccacaaatca
     3721 acttttgcct taccgctttc gccgccacca ccatttccgg attgcttttg caacttccca
     3781 ctttcagcaa tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat
     3841 ttccgttgat taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac
     3901 gctgggcgat cgccagtctg ctgacactgg ctacctgcgt cggcgtagtg atggcactga
     3961 cgatttgggg gctacgcgga tgggtgtgat cgaatttctg ttagcgttgg cgcaggatat
     4021 gatcctcgcc gccattcctg cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc
     4081 gttacgctgg tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac
     4141 cagcgggctg aatattgagt ggtcaacttt tatggcttct atgctggtcg gcaccattgg
     4201 tattcaatgg tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat
     4261 ccctatgttc ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca
     4321 gttaggttac agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc
     4381 gattgttggt gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg
     4441 ccctcgcgta taaaattgcc tcgcttaagc acacggatga gagacagcct cctctcctcc
     4501 gtgtgttact ataaaagtta tctcccttct cgttcatcgt tccatatttg agaaacagta
     4561 tgtcttccag agttttgacc ccggacgtcg ttggtattga cgccctggta cacgatcacc
     4621 aaaccgttct ggcaaaagct gaaggcggtg tggttgccgt atttgctaac aatgccccgg
     4681 cgttttatgc cgtcacgcct gcacgcctgg ctgaactgct ggcgctggaa gaaaagctgg
     4741 cgcgtccggg aagcgatgtc gctctggacg atcaactcta tcaggaaccg caagccgctc
     4801 ccgttgctgt ccccatgggg aaattcgcca tgtatccgga ctggcaaccc gatgccgact
     4861 ttatccgcct ggcggcgcta tggggcgtgg cgctaagaga gccggtgacc accgaagaac
     4921 tggcctcatt cattgcctac tggcaggcgg aaggtaaagt ctttcaccat gtgcagtggc
     4981 aacaaaaact ggcgcgcagc ctgcaaatcg gtcgtgccag caacggcgga ctgccgaaac
     5041 gagatgtgaa tacggtcagc gaacctgaca gccaaattcc accaggattc agagggtaac
     5101 gatgaaaaac gttggcgacc tgatgcaacg cctgcaaaaa atgatgcctg cccatatcaa
     5161 acctgcattc aaaacgggtg aagagctact ggcctggcag aaagaacaag gagcgatccg
     5221 ctccgccgct ctcgaacgtg aaaatcgggc gatgaaaatg cagcgcacct ttaaccgctc
     5281 cggtattcgg ccactgcatc agaactgctc ctttgagaac tatcgcgttg agtgtgaagg
     5341 gcagatgaat gcgttaagca aagcgcgcca gtacgtcgaa gagtttgacg gcaacatcgc
     5401 cagcttcatc ttttctggta agccaggaac cggcaaaaac catctggcgg cggcaatctg
     5461 caacgagctg ctgctacgcg gtaaatccgt attgatcatc accgtggccg atattatgtc
     5521 ggcgatgaaa gataccttca ggaatagcgg taccagtgaa gaacaactgc ttaacgatct
     5581 gagcaacgtt gatctgctgg tgatcgatga gatcggcgtg cagaccgaat cgaaatacga
     5641 aaaagtgatc atcaaccaga tcgtcgatcg ccgctcttct tccaaacgcc caaccgggat
     5701 gctgaccaac agtaatatgg aagagatgac caagttgctg ggcgaacgcg tgatggaccg
     5761 tatgcgcctg ggtaacagtt tgtgggtgat cttcaactgg gatagctacc gtagccgggt
     5821 aacaggtaaa gagtattaat ccccgtttct ggcggtatac tgcgacacaa ttccaccttg
     5881 agtaatgatg atgaaaactg ttaaacatct tctgtgctgt gccattgccg ccagcgcatt
     5941 aatttccacc ggggtgcatg ctgcgtcctg gaaagatgcg ctctccagcg ccgccagcga
     6001 acttggcaac caaaacagca cgacacagga aggcggttgg tcgctcgcgt cattaactaa
     6061 cttgcttagc agcgggaacc aggccttaag cgcagataac atgaacaacg ccgcaggcat
     6121 tctgcaatac tgcgcgaagc aaaagctggc gtcggtaacc gatgccgaaa acatcaagaa
     6181 ccaggtgctg gaaaagctgg gcctgaacag tgaagagcaa aaagaagaca ccaactatct
     6241 ggatggcatt cagggtttgc tgaaaaccaa agatggtcag caactcaatc tggataacat
     6301 cggaacgact ccgctggcag aaaaggtgaa aaccaaagcc tgcgatctgg tgttaaagca
     6361 ggggctgaac ttcatttcct gaaccgcgaa acgccgcgtt ttagctgtta cccggctgac
     6421 gcggctctct cctgcccttt tccctgctcc tggacggttt tccccctgtt acacgggaag
     6481 cgaccgcgat caaaaaagtc tgtttataaa ctaattccta acaacatcgc atttttttga
     6541 cactagaatt gcagcaatat ggcgacgcca ttacattgtg cttctcaaaa agcttaaacg
     6601 ttggctaaac ggcccggttt agtgaggatc gcccgttgtc agaactactc tctttcgccc
     6661 tttttctcgc ctctgtgctg atttacgcat ggaaagcggg acgtaacacc tggtggtttg
     6721 cagccacatt aacggtgctg gggctatttg tcgttttaaa tatcaccctg tttgccagcg
     6781 actattttac tggcgatggt attaacgacg cggttctcta taccttaacc aacagcctga
     6841 ccggtgctgg cgtcagcaaa tacatcctgc cgggtatcgg cattgtgctg gggctgacag
     6901 cggtgttcgg tgcgctgggc tggatcctgc gccgtcgtcg ccatcatccg caccattttg
     6961 gttacagcct gctggcgctc ttactggcgc tgggttcggt ggacgccagc ccagcatttc
     7021 gtcagataac ggaactggtg aaatcccagt cacgcgatgg cgacccggac tttgcggctt
     7081 attataaaga gccgtcgaaa actatccctg acccgaaact caacctggtt tatatctacg
     7141 gcgaaagtct cgagcggacc tattttgata acgaagcttt cccggatctc acgcctgaac
     7201 tgggcgcgtt gaaaaatgaa ggcctggatt tcagccacac gcagcagctg ccaggaacgg
     7261 attacacgat tgcgggcatg gtggcttctc agtgcggcat accgctgttt gccccctttg
     7321 aaggcaacgc ctccgcctct gtctccagct tcttcccgca gaacatctgt ctgggcgata
     7381 tcctgaaaaa ctcgggttat cagaactatt tcgtgcaggg cgcgaatctg cgttttgccg
     7441 gtaaagatgt gttcctgaag tcgcatggct tcgaccactt atacggctca gaagagctga
     7501 aaagcgtggt ggccgacccg cactatcgca acgactgggg attctacgac gataccgttc
     7561 tcgatgaagc gtggaaaaag tttgaagagc tttcccgctc aggtcagcga ttctcactgt
     7621 ttaccctgac agtcgatacc catcacccgg atggttttat ctcgcgtacc tgtaaccgca
     7681 aaaaatatga ttttgacggt aagccgaacc agtcattcag cgcggtaagt tgcagccagg
     7741 agaatatcgc ggcgtttatc aacaaaatca aagcgtcacc gtggtttaaa gataccgtca
     7801 tcgtcgtctc ttctgaccat ttagcgatga acaacacggc gtggaaatac cttaataagc
     7861 aggatcgcaa taacctgttt tttgtcattc gtggcgacaa gccgcagcaa gagacgctgg
     7921 cagtgaagcg taacacgatg gataacggcg cgacggtgct ggacattctc ggtggcgata
     7981 actatctcgg acttggtcgt agcagtttat ccgggcagtc aatgtcggaa atcttcctca
     8041 atatcaaaga gaaaacattg gcgtggaagc cggatatcat ccgcctgtgg aaattcccta
     8101 aagagatgaa agagttcacc atcgaccagc agaaaaacat gattgccttc tcgggtagcc
     8161 atttccgttt gccgctgctg ttgcgggttt cagacaaacg cgtggaaccg ctgccggaaa
     8221 gcgaatactc agcaccgctg cgtttccagc tggccgattt cgctccacgc gacaatttcg
     8281 tctgggttga ccgttgctat aagatggcac aactctgggc tccggaactg gcactctcca
     8341 ccgactggtg tgtctcgcaa gggcagcttg gcggtcagca aattgttcag catgttgaca
     8401 aaacaacatg gcagggcaaa acggcattta aagatacggt catcgacatg gcgcgttata
     8461 aaggcaatgt cgatacgctg aaaattgttg ataacgatat tcgctacaaa gccgacagtt
     8521 tcatcttcaa cgtcgccggt gcgccagaag aggtgaaaca gtttagtggg atttcccgtc
     8581 cagagtcgtg gggccgctgg tccaacgcgc agctgggcga tgaagtaaaa atcgagtaca
     8641 agcatccgct gccgaagaaa tttgacctgg tgattaccgc caaagcatac ggcaataacg
     8701 ccagccgtcc tattccggta cgcgtaggca atgaagaaca aactcttgtg ctgggcaatg
     8761 aagtgaccac caccacactg catttcgata acccaaccga tgccgacaca ctggtaattg
     8821 tgccgccgga acctgtctca accaacgaag ggaatatcct cggacactcg ccgcgtaagc
     8881 tcgggatcgg catggtggaa attaaagttg tggaacgtga agggtaattt ctgactgatg
     8941 tgtaggtcgc ataaggtgcg acctacacat cttctccggc ctacaaaaca tcattgagaa
     9001 acagaaaaaa aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc
     9061 gccttatcag aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc
     9121 gtaggtttct gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cggcagtgat
     9181 ttttccttcc gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct
     9241 gcccatcatc gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt
     9301 aaacaggccg acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat
     9361 cgcatgttga ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc
     9421 ccgtagctgc gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt
     9481 tgccacatgt tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc
     9541 tttagcgatt gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc
     9601 gccgggagca atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa
     9661 tgcggccgcc tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa
     9721 atactcacta aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt
     9781 ggtacgcccg cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac
     9841 ttgctgacca tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat
     9901 ttcatggcct aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat
     9961 atcggtaccg caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat
    10021 aggtatctcg cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat
    10081 cgtagacatg attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat
    10141 ggcagcggaa cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg
    10201 ttttaatgga ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg
    10261 attaaccagg tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa
    10321 agtgcgctgg cggaaatgta caacatcagc cgcacaacag tgcgtcatat tctcagccac
    10381 ttacgcgaat gcggcgtctt aacgcaggtt ggaaacgact atgttattgc gcgtaaacct
    10441 gaccatgatg acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt
    10501 gagcaggctt ttttcacgat gataaaccag cgtcagttac gccctgggga aactttttct
    10561 gaactgcaac tggctcgggc cgcgggtgtc agcccggtgg ttgtaagaga atatctttta
    10621 aaattcggcc gttataatct tattcaaagc gaaaaacgcg gccagtggag catgaagcaa
    10681 ttcgatcaat cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca
    10741 cttcagcact ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg
    10801 ctcgaacgtc atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa
    10861 cttgacagag attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa
    10921 tcgcttgaga tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt
    10981 aaacagcgca atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc
    11041 cgcagtgacc ttgatgcccc ccttgcgctc cgtaaccatt tgaattcagc taaacaatca
    11101 atgattcgat ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc
    11161 aaactattag cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc
    11221 tatgctcaca atcagtcgac taaaaatttt tttgtgagcg gtagtaaagt cctaaaactt
    11281 taacctgccg ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata
    11341 aatacaggag tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac
    11401 tccatcttcg tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg
    11461 aattaaacgc attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta
    11521 tctcgaccgc agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag
    11581 tgccaccgaa atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact
    11641 tccttgcggc ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt
    11701 cttctggtca ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt
    11761 ggtgcgtatc ggtatgggga ttggtgaagc gccgatgaac ccatacggtg taaaagtcat
    11821 taacgactgg ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc
    11881 taccattggc gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg
    11941 gcgcgggatg tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat
    12001 gctctatcgc aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc
    12061 aggtagcgtc aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg
    12121 taaccgtaca atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct
    12181 gtatctggcc tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac
    12241 agggttgatg gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta
    12301 cgttactgac tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat
    12361 tattgccggg atgttctgtt ctgccgcctt tacgctgata gtaccacaag cgacaacatc
    12421 catgacggcg gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc
    12481 ctgctggggc ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat
    12541 ccagaacttt gccagcttca tctgcgcctc ttttgcgccg atcattactg gttttattgt
    12601 tgataccacc cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg
    12661 ggcactggcg tacatcttcc tggttcgtca gccgatcaac gacccacgta aagattaatc
    12721 tccttatgcc cgataacatt ttgcttatcg ggcattttca tggcgattaa aatgtttccc
    12781 agttctcctc gctatctgcc acggccattt tacgcggcgc agctggcgtc acggttttta
    12841 ccacagccga tgtttcacgc tgctgttgct gctgttgctg aatccggaac actgccacag
    12901 ctttggtcag gcgactggcc tgctcttcca gcgcggcggc ggcagcggca gattcttcca
    12961 ccagcgcggc gttctgttga gttacccggt ccatctcagc aaccgctaag ccaacctgat
    13021 cgataccacg gctctgctca tcagaagcag aagcaatttc gcccataatg tccgtcacgc
    13081 gggtcacggc gctgacaatc tccgccattg tttccccggc gctttcgacc agcgtagagc
    13141 caacatccac tttccccacc gagtcttcaa tcaggctttt aatttcacga gccgcctgag
    13201 cgctacgctg ggccagatta cgcacttctc ccgcaaccac cgcaaaaccg cgcccttgct
    13261 cacccgcacg cgctgcttca accgccgcgt tcaaagccag aatattggtc tggaaggcaa
    13321 tgccgtcaat tacgctgata atatcggcga ttttctgcga actggtggag atatcgcgca
    13381 tagtctgcac cacgttatct accactttgc cgccccgttg cgccgtttca gaagcactta
    13441 atgccagatg gctggcctgg cgcgcattct cggcgttctg tttcaccgtt gcggtcagtt
    13501 gctccatgct ggctgccgtc tcttccagcg aagcggcctg ttgctcggtg cgcgaagaga
    13561 gatcgttatt gccggtagcg atttcgctgg caccgctata gatggcattg gccccgttgc
    13621 gcacatcacc gacggtacgc atcagctctc cctgcatatg gcgcaaactc tctgccagtt
    13681 gccccatctc attagagcca tccacctcaa tcggtttcac cagatcgccg cctgcaatat
    13741 gacggatgct gtcaatcagg cgattcattg gcgctaccag cgaggcttta ataccgaacc
    13801 agacggcgaa gatgaccgcc agtacgacga tcatcacgcc caccagaatc cacatcgcct
    13861 ggctgtagga ggcattgtta tcgctgacgg cgatatcata gagccgatcg ttttgctcca
    13921 tgtaagccac atactgcttc tcgaaaccgt cttgatatcc ctgggtcggc tgatcaaaga
    13981 actcgttgat tttgcctgca cctaacagtt ggatcagctc cgccagcgca ttgtgataaa
    14041 tatcgtaatt acgtttgatc tctgccgctg cggcggtgct ctgacgcggg tcacgcggca
    14101 acgcttcgta atccgcccag tttttttccg cctgtttcag cgaaatactg gcactctgca
    14161 tcagctcagc aacggttgaa ccgctaccaa tattattctg atccatcatg tagcggatac
    14221 ccgcgcggtt gagggtgtta cgcgtctgca acaacgcgac ccagctgcca ttcagcgtgg
    14281 attgctgctg gcgaatggtt tgtaaaacag tgaaattttc tttgtcattc tttaaggcat
    14341 taaagaacag accgcctgat gtcagttgta aaaggccaaa aacggccaaa accagcagta
    14401 agctggtcac gatcttgata cgttttaaca tggtttctct ttcctgtgga ccgatgcaag
    14461 attttcggcc tggaaagcaa aaactttatg aaaaggtgta acccgccatc catggaaaaa
    14521 ccaggcgctg gttatcagat actaaaaagt tattcattat cgcccgggat ggcttcttgt
    14581 cgggaattgc ccagagcaat aaattcctct aacagatggg ttaactgttg cattttctcg
    14641 gcagtaaact gagcttcaat ctgccgatag gcttcttcaa tctgtgtctg agcgcggtta
    14701 tacagcgcct gcccctcttt ggttagcgag atatagagct tccgctgatc attgatcggc
    14761 tttaatcgca acactaaacc gtcgcgttcc atccgcgtga ggatcccggt caggcttggg
    14821 cgcaaaatgc aggcgcgata cgccagatcg tgaaaatcca ttgatgggct ttccgccaga
    14881 atacgcacga tgcgccactg ttgctcagtc aaattatgcc gtttaacgat tgggcgaaaa
    14941 taactcatcg ccgcttctcg cgcctggagc aacgcaatgg ttagagagtc gtgcattatc
    15001 tttcccctga aaaattcaaa attattaata tagaaacagt taatcatcac gttaccggga
    15061 ctgcgctgcc cctgcaattc ttcatttta
//
LOCUS       DAZGTR010000105        14927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGTR010000105 DAZGTR010000000
VERSION     DAZGTR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.107"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(239..1291)
                     /gene="fbaB"
                     /locus_tag="ACHLCY_003638"
     CDS             complement(239..1291)
                     /gene="fbaB"
                     /locus_tag="ACHLCY_003638"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP0370816.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1548..2825
                     /gene="yegT"
                     /locus_tag="ACHLCY_003639"
     CDS             1548..2825
                     /gene="yegT"
                     /locus_tag="ACHLCY_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP0370817.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2822..3826
                     /gene="yegU"
                     /locus_tag="ACHLCY_003640"
     CDS             2822..3826
                     /gene="yegU"
                     /locus_tag="ACHLCY_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP0370818.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3823..4788
                     /gene="yegV"
                     /locus_tag="ACHLCY_003641"
     CDS             3823..4788
                     /gene="yegV"
                     /locus_tag="ACHLCY_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0370819.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4762..5508)
                     /gene="yegW"
                     /locus_tag="ACHLCY_003642"
     CDS             complement(4762..5508)
                     /gene="yegW"
                     /locus_tag="ACHLCY_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0370820.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5560..6378)
                     /gene="yegX"
                     /locus_tag="ACHLCY_003643"
     CDS             complement(5560..6378)
                     /gene="yegX"
                     /locus_tag="ACHLCY_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP0370821.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVIYHTNLAGYVNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(6443..7243)
                     /gene="thiD"
                     /locus_tag="ACHLCY_003644"
     CDS             complement(6443..7243)
                     /gene="thiD"
                     /locus_tag="ACHLCY_003644"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP0370822.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7240..8028)
                     /gene="thiM"
                     /locus_tag="ACHLCY_003645"
     CDS             complement(7240..8028)
                     /gene="thiM"
                     /locus_tag="ACHLCY_003645"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP0370823.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLSHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8063..8159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8251..8523)
                     /gene="rcnR"
                     /locus_tag="ACHLCY_003646"
     CDS             complement(8251..8523)
                     /gene="rcnR"
                     /locus_tag="ACHLCY_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP0370824.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8643..9467
                     /gene="rcnA"
                     /locus_tag="ACHLCY_003647"
     CDS             8643..9467
                     /gene="rcnA"
                     /locus_tag="ACHLCY_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP0370825.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVVSKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWHGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9686..10024
                     /gene="rcnB"
                     /locus_tag="ACHLCY_003648"
     CDS             9686..10024
                     /gene="rcnB"
                     /locus_tag="ACHLCY_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP0370826.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10106..11140)
                     /gene="yehA"
                     /locus_tag="ACHLCY_003649"
     CDS             complement(10106..11140)
                     /gene="yehA"
                     /locus_tag="ACHLCY_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP0370827.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKRYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(11154..13634)
                     /gene="yehB"
                     /locus_tag="ACHLCY_003650"
     CDS             complement(11154..13634)
                     /gene="yehB"
                     /locus_tag="ACHLCY_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0370828.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGGWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(13650..14324)
                     /gene="yehC"
                     /locus_tag="ACHLCY_003651"
     CDS             complement(13650..14324)
                     /gene="yehC"
                     /locus_tag="ACHLCY_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0370829.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(14405..14902)
                     /gene="yehD"
                     /locus_tag="ACHLCY_003652"
     CDS             complement(14405..14902)
                     /gene="yehD"
                     /locus_tag="ACHLCY_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0370830.1"
                     /translation="MSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPT
                     TVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTV
                     GYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVM
                     RVAQD"
BASE COUNT         3670 a         3759 c         3507 g         3991 t
ORIGIN      
        1 gatcacaaaa acgacaatgt gaaaattgtt cgaggagtaa aaggcaaaaa aaaacggcct
       61 cccgataggg aagccgtagc aaagtgcgcg tgtttttatg ccggatgcgg tgtaaacata
      121 cgtggcagtg ctatgtaggc atgataagac gcttccgcgt cgcatcaggc ataggttgcc
      181 ggatgcagtg taaacgcctt atccgtccta cggggtggtg ctgttcgtat gaggcgtatc
      241 aggcaatagt aattttgcta tcgagataaa cgtcctgcac ggcattaatc agtttcacgc
      301 cgtcagccat cgatttcttg aacgccttac gtccaagaat cagccccatt ccgccagcgc
      361 gtttgttgat aaccgcagta cgcactgcat cgctgaggtc agtttcaccg cccgcagcac
      421 cgccggagtt tatcaaccca gcccgaccca tatagcagtt agctaactga taacgcacca
      481 gatcaatcgg gttttcgctg gtcaatttgc tgtaaacacg atcgtcggtg taaccgtaat
      541 taattgcttt atagccgccg ttattttccg ccattttttg tttgacgata tctgcaccga
      601 tggttgccgc cagatggttt gcctgaccgg tcaggtcggc ggaaacatgg taatcaacgc
      661 catctttctt aaaggcggag ttacgcaaat aggcccacag cactgtcacc atacccagct
      721 cgtgcgcacg ttcaaaagcc gcagaaattt cttcaatctg gcgacgtgac tcttccgagc
      781 caaaatagat agtcgcacca accgcaaccg cgcccatgtt gaacgcctgc tccacgctgg
      841 catacagcgt ttgatcgtag gtattcgggt aacttagcgt ctcgttgtga ttaagtttga
      901 cgaggaatgg aatgcgatgc gcataacgcc gcgataccga cgccagcacg ccgtaagttg
      961 acgccacaca gttacagccc gcttcgatcg ccagttcaac aatgtttttc gggtcaaagt
     1021 agagcgggtt agcagcaaat gaagctccgg cagagtgctc aacgccctgg tcaaccggca
     1081 gaatagaaag atatcctgtg ccagccagac gcccggtgtt gtacaacgtc tgcatattac
     1141 gtaacaccgc tggcgggcga ttattgtcaa tcattacgcg gtctacgtag tcatgtccgg
     1201 ggagataaag ctggtcagaa ggaattgtca tacaacggtg ctgtaaaagg ttgtcggcgt
     1261 ctttgccaag caactgcgca atatctgtca ttactatgct cccgtaaatt ccgattggat
     1321 atcggctatg gattgtcctg gcccgccttt tgcgggcaat cataatcctg gtcgttacgg
     1381 gcgagttttt ccatcttttg gatgtttttt cagcgttttc tgttggctcg attcatcaga
     1441 aaaaatgtta gcgcggtcaa gttttcaccg caaaggtatt taaaaggtat tattaagtgg
     1501 tattgtcatc gcgtacctta cattacctgt catgaaggaa ttaaaagatg aaaacaacag
     1561 caaagctgtc gttcatgatg tttgttgaat ggtttatctg gggcgcgtgg tttgtgccat
     1621 tgtggttgtg gttaagtaaa agcggtttta gtgccggaga aattggctgg tcgtatgcct
     1681 gcacagccat tgcggcgatc ctgtcgccaa ttctggttgg ctccatcact gaccgctttt
     1741 tctcggcgca aaaagtgctg gcggtattga tgttcgcagg cgcgctgctg atgtatttcg
     1801 ctgcgcaaca gaccactttt gccgggttct tcccgttact gctggcctac tcgctaacct
     1861 atatgccgac cattgcgctg actaacagta tcgcttttgc caacgtgccg gatgtggagc
     1921 gtgatttccc gcgcattcgt gtgatgggca ctatcggctg gattgcctct ggtctggcat
     1981 gtggtttctt gccgcaaatg ctggggtatg ccgatatctc accgactaac atcccgctgc
     2041 tgattaccgc cggaagttct gctctgctcg gtgtgtttgc gtttttcctg cccgacacgc
     2101 cgccaaaaag caccggcaaa atggacatta aagtcatgct cggcctggat gcgctgatcc
     2161 tgctgcgcga taaaaacttc ctcgtctttt tcttctgttc attcctgttt gcgatgccac
     2221 tggcgttcta ttacatcttt gccaacggtt atctgaccga agttggcatg aaaaacgcca
     2281 ccggctggat gacgctcggc cagttctctg aaatcttctt tatgctggca ttgccgtttt
     2341 tcactaaacg ctttggtatc aaaaaggtat tattgcttgg tctggtcacc gctgcgatcc
     2401 gctatggctt ctttatttac ggtagtgcgg atgaatattt cacctacgcg ttactgttcc
     2461 tcggtatttt gcttcacggc gtaagttacg atttttacta cgttaccgct tacatctatg
     2521 tcgataaaaa agcccccgtg catatgcgta ccgccgcgca ggggctgatc acgctctgct
     2581 gccagggctt cggcagtttg ctcggctatc gtcttggcgg tgtgatgatg gaaaagatgt
     2641 tcgcttatcc ggaaccggta aacgggctga ctttcaactg gtccgggatg tggactttcg
     2701 gcgcggtgat gattgccatt atcgccgtgc tgttcatgat ttttttccgc gaatccgaca
     2761 acgaaattac ggctatcaag gtcgatgatc gcgatattgc gttgacacaa ggggaagtta
     2821 aatgaaaaca gaacgtattc tcggtgctct ttatgggcag gcgttagggg atgcgatggg
     2881 gatgccctcc gagctttggc cacgcagccg cgttaaagca cactttggct ggattgaccg
     2941 ttttctgcct ggaccaaagg agaataacgc ggcctgttat tttaaccgcg ccgaattcac
     3001 cgacgatacc tcgatggcgc tgtgtctggc ggatgcgtta ctggaacgtg aaggcaagat
     3061 cgatccggat ctgattgggc gtaatattct cgactgggcg ctgcgtttcg acgcctttaa
     3121 caaaaacgta ctaggtccga cctcgaagat tgcgcttaac gccattcgcg acggtaaacc
     3181 cgttgctgaa ctggaaaaca acggcgtgac caacggcgca gcgatgcgcg tctcgccatt
     3241 aggttgtttg cttccggcgc gtgatgttga ttcctttatt gatgatgtgg cgctggcgtc
     3301 gagcccgacc cataaatccg atctggcagt tgcaggtgcg gtagtcatcg catgggcgat
     3361 ttctcgtgcc attgacggag aaagctggtc agcgattgtt gattccctgc cttcaattgc
     3421 gcgacatgca cagcaaaaac gcatcactac cttcagcgcc tcactggcag cacgtctgga
     3481 gattgcgctg aaaattgtgc gcaatgccga cggcaccgaa tccgccagcg aacagcttta
     3541 ccaggtcgtt ggcgcaggta ccagcactat tgagtccgtt ccgtgcgcca ttgcgctggt
     3601 tgaactggca caaaccgacc cgaatcgctg cgccgtcctg tgcgctaacc ttggcggcga
     3661 cacagacacc atcggcgcta tggcgacggc gatttgcggc gcgttgcatg gcgttaacgc
     3721 tatcgatcct gcattaaagg cggaactgga tgcggtaaat cagcttgatt tcaaccgcta
     3781 tgccacagcg ctggcgaaat atcgtcaaca acgggaggcg gtatgagcgg cgctcgatta
     3841 cacacgctgc tgcctgaatt aacctcgcgt cagccggtga tggtcgtcgg cgcggcggtc
     3901 attgatgtga tcgccgacgc ttatgccctc ccctggcgtg ggtgcgatat cgaactgaag
     3961 cagcagagcg ttaacgttgg cggctgcgca ctgaatattg ccgtggcgtt aaagcgcctc
     4021 ggcatcgaag cgggtaatgc cttgccgctc ggtcagggcg tgtgggcgga gattattcgc
     4081 aaccggatgg cgaaagaggg gttaatcagc ctgatcgata acgccgaagg cgataacggc
     4141 tggtgtctgg cgctggttga gccggatggc gaacgcactt ttatgtcatt cagcggtgtg
     4201 gaaaatcagt ggaatcgcca gtggctggcg cgattaaccg ttgcgcctgg cagcctgctc
     4261 tatttttccg gttatcaact ggcctcgccc tgcggcgaat tgttagtgga atggctggaa
     4321 aagctgcaag acgtgacgcc gtttatcgat tttggcccac gtatcggcga tatcccggac
     4381 gcattactgg cgcggatcat ggcctgtcga cctttagtat cactcaatcg tcaagaggct
     4441 gagattgccg ccgaacgttt tgctttatcc gcagagataa caacacttgg caagcaatgg
     4501 caggagaaat ttgccgcgcc gttgatcgtt cgtctcgata aagagggtgc atggtatttc
     4561 agcaacgacg cttctggctg cattccggca tttccaacgc aagttgtaga caccattggg
     4621 gcgggcgaca gtcatgcagg tggcgtactt gccgggctgg cctctggtct gccactggcg
     4681 gatgccgtat tactgggcaa tgcagtggcg tcgtgggttg tcgggcatcg gggcggtgat
     4741 tgtgcgccaa cgcgcgagga actactcctc gcacacaaaa acgtatagat cgctgcgaca
     4801 gtggctaatg ctgtactcaa taggccgttg ctgttggtca agcgccactt gcttgatcac
     4861 cagcaccggt attttgctat ctaactgaat atgtgactga aactcggcat ccggcatccg
     4921 ggcgctaacg cgggaacgcg tacgttgtgg gtaaatgtgc tggctgcgga aatagtcata
     4981 aagtgaaatc ccgatggcat caacatcatg aattaaatgc gccggaaccc acgattcctc
     5041 aatcgatacc gcttcttcat cgacatagcg aatacgctta agcaaaaaga catcacttcc
     5101 tgcttcgacc gccagctgct gcgcgacttc ttcgggacat ttcacgacac gcttgttaac
     5161 ccatagcgta tcgggctttt tcccgcgcaa taccacctgc tgagaaaaac ctcgcgcttc
     5221 tttcaacgag tattcgaaga tgttgttgat ctgcgtgccg taaccgcgcg aacgcgtcac
     5281 cacaccctct tcttccagcg cctgcatcgc cttgcgcacc gtaatgcgcg aaacgccggt
     5341 taactgactt aaatcacgct caccgggcaa aatattgcca tgctccagca ccccgctgcg
     5401 cacggcattt tttaccgttt cggcaaactt aatataaagc ggcgtgttat ccgccgctaa
     5461 aatacgctcg ttcagttggg caatcagctg ggtatgcgct tgttccattt gtctttttcc
     5521 tgccggggag tctggtggga gtatatcact gcttcttatt taaggcgttt ctttaatccc
     5581 atcaacgaat gcctgcaact cctccaccgt gccatgaaat acattaaaat ccaccggacc
     5641 attgatgcca tctacctgtc cacggtcgga atgctgccag aagcgccagg ccatgccgtc
     5701 attgtccgga cgacgttgat aatagtgagc cacccaccac ggatattcat tgacatagcc
     5761 cgccagattg gtgtgataaa taacggctcc tgagtaaata atcggctttt ttcccgtact
     5821 tttttcgacc atttttagcc actgacttac tcgtttgcgt aattcttttg ccgataattt
     5881 tccccgttct tctacgtcca gtaccgcagg gaaatcgcct tgtgagaaat ccaccgtttg
     5941 cagaaataat cgtgcctgaa ccggcgctgc taccgacggg gagaaataat gatacgcccc
     6001 gcgcagcagg ccattttcgc ggcttagttg ccagttacgc gaaaaatacg ggtccaccag
     6061 cttttcgcct tccgtcgcct taataaaagc aaactgtaag cggataccgt tgtcgcgcat
     6121 ttttgccacc cgctgccagt cgatccgctc ctgccagcga gaaacatcta ttccgtgaat
     6181 ggtgtaactg gcaggaatgc gaatagcgaa agatttaacc gggcgataac cgtagaaatg
     6241 gatgtagtct gtaatctgaa ctgctgtcga atagaaaaaa ttcaccgtct gacgcggata
     6301 aattgcaacg atggaaatta gcccaagagc gcataataga gaggtgagtt tttttcgact
     6361 ggtaattctt agctgcattg gtttcatccc tgaatgtcag tgccaaaggc tgacaataac
     6421 caaagcagct atagtacggt gctcaccacc aggcgtggaa gtggtgaacc ggaccaatac
     6481 cgtgaccaac ttccagcgtg tcggcctggg ctaatgctga tgaaagccag ctttttgcct
     6541 cctgtacggt gtcagcccag tttgtatggc gcgggcgtag tgcagccaac gccgcagaga
     6601 gcgtacaacc agtgccgtgg gtgtttttgg tcataatgcg cggtgcggta aaccgttgtt
     6661 caccctcgcg ggtaaacagc cagtccgggc tttgctcatc atccagatga ccacctttca
     6721 ttagcactgc gccacagccc atcgccaaca gcgatcgccc ttgttccagc atttcctgtt
     6781 cggtgcgcgc gtgtggcgcg tcgagcaagg cggcagcttc gggcaagttt ggcgttatta
     6841 atgaaacctg tggcaataat cgactgcgca gcgtagcaac cgccgaaggt gaaagcagcg
     6901 ggtcgccgct ttttgccagc ataacggtgt cgagtaccac gttttggatc tgataacgtt
     6961 gcaaccgttc tgccaccgct tcaacaatat cggtttccgc caacataccg attttagtgg
     7021 tgtcgattcg cacatcgctg aacaccgaat cgagctgggc ggcgacaaaa tcaggctcaa
     7081 tgcgatacac cgactgtacg ccacgggtat tttgcgccac cagtgcagta ataactgagc
     7141 aaccataagc gccaagtgcc gagaaggttt taagatcggc ctgaatcccc gcaccaccac
     7201 tgggatcagt accggcaatc gtcagagcgt taattcgttt catgcctgca cctcctgcgt
     7261 caattgccag agcgcatcaa ggaaatgtgg aacaaaactg cctggcccct cgcttctggc
     7321 gactgcgcgc tctccggctt gtttcatcca gtgacaggca gatgcgacat tttccagcgt
     7381 atcgcctggt aacgcacagc aggcagcgac aaccgccgat aatgcacagc cagttcctac
     7441 cactttggtc attaacggat cgccaccgtg aatgccaacg gcgcgatggc catcggtaac
     7501 ataatccatc tcgccagtga ccacgacgat tgcgccagtt tcccgtgcca gtgtttgtgc
     7561 agcgggtatc gcgttagctg cggcgtcagt ggtatccact ccccgtccac cattagcaat
     7621 gccagctaat gccatgattt ccgaagcatt accacgtatg gcggttggct tatgagacag
     7681 aagttccaga caaaaacggc ggcgataatc gagtgcacca accgctactg gatcaagcgt
     7741 ccagggtgtt tgagagcttt ttgcttgctc aacggcggca cacatcgact gtgcgcgtag
     7801 ctgcgtcagc gtgccaacgt taatcagcaa cgcactggca atagccgcaa actgactggc
     7861 ctcttcggtt tcgataacca tcgctggcga tgcaccgagc gccagcaagg tattggcagt
     7921 aaaggtttgc accacatcgt tggtcatgca gtgcacaaga ggggaatgtt ggtgaaaaag
     7981 gtgtaacgcg tgcgcagatt gcgctgaact cagcaggtcg acttgcatag tttgctcctg
     8041 ccataacgtg aagaagcaat gacctggtgg tccgtgactt ccctacgctg gcattatcca
     8101 gatcaggtga tacgggtatt tctcagcctt cacgcagaag ggcaccccga gtcgtttggt
     8161 tgcgatgata aatcgcagag gaggatggta atgtccagcg cacgcgttgt aaacgaggaa
     8221 aaacggcagt tttacaatcg cgttaataaa ttatttgata tatgaatcca gcaccttcag
     8281 aacgacatcc agatcttctt cacgttttag ctcatccccc tggtgaacga tgtgttccgt
     8341 cagatgacct ttaatcactt cccgcatcag accgtttacc gcgccacgga tagcagcaat
     8401 ctgttgtaaa actgcagcgc attcgtgcgg ttcgtcgagc attttcttga gcgccacgac
     8461 ctggccctga atcttactgg cacgcgcttt cagtttctgt ttatcacgga ttgtatgaga
     8521 catggcaaca cctggttaac aagaatatga aaaatcatag cactattaat ctactggggg
     8581 gtagtatcag gtactggggg gagtagaatc agattgccga attaatacta agaattatta
     8641 tcatgaccga atttacaact cttcttcagc aaggaaacgc ctggttcttc atccccagcg
     8701 ccatcttact tggtgcgctt catggcctgg aaccagggca ctcaaaaacg atgatggcgg
     8761 cgtttatcat cgccatcaaa ggcaccatta aacaggcggt gatgctcgga ctggcagcaa
     8821 ctatttcgca taccgcagtg gtctggttga ttgcctttgg cgggatggtg gtcagcaagc
     8881 gctttactgc tcaatcagca gaaccgtggc tccagttgat ttccgcagtg atcattatta
     8941 gcaccgcgtt ctggatgttc tggcgtacct ggcacggcga gcgcaactgg ctggagaata
     9001 tgcacgagca tgatcatgag catcatcatc acgatcacga agatcaccac gaccatggac
     9061 atcatcacca tcacgaacat ggcgagtatc aggatgctca tgcacgagcc catgctaatg
     9121 acattaaacg acgctttgat ggtagagagg tcaccaactg gcaaattttg ttatttggct
     9181 taaccggtgg ccttatcccc tgcccggcag caattaccgt gctgttgata tgcattcagt
     9241 tgaaagccct gacactgggc gcaacactgg tcgtcagttt cagcattggc ctggcgttaa
     9301 cgcttgtcac cgtaggcgtt ggcgcagcaa tcagcgttca gcaggtcgca aaacgctgga
     9361 gcggatttaa cactctcgct aaacgcgccc cctatttttc cagtctgttg attggcttag
     9421 tcggtgtgta tatgggcgta catggcttca tgggcataat gcgataactc aatcctttag
     9481 gcttgcatgt tatcttcagc ccaggatgat tcttaaatca gctatttccg ctgactctct
     9541 gctcgctaag gatgctttta gggcatcctt tcatttacac tttttacgaa atcatgggat
     9601 cactaacaaa atatcgcttg tcagttatat tgtatggcag gaaagatatg cgactgatat
     9661 tacagatccc caaagtggag agtttatgac cattaaaaat aagatgttgc tgggtgcgct
     9721 tttgctggtt accagtgccg cctgggccgc accagccacc gcgggttcga ccaatacctc
     9781 gggaatttct aagtatgagt taagcagttt cattgctgac tttaagcatt tcaaaccagg
     9841 cgataccgtc ccagagatgt accgcaccga tgagtacaac attaagcagt ggcagttacg
     9901 taacctgccc gcgcctgatg ccgggacgca ctggacctat atgggtggcg cgtacgtgtt
     9961 gatcagcgac accgacggta aaatcattaa agcctacgac ggtgagattt tttatcatcg
    10021 ctaaaaaaag ccccctcatc atgaggggga aatgcagaca ccttgttatt ttttattatt
    10081 agccacttgc tcgtcttgct tgttattagt cgtatttcac gttgattaat gcggttgcct
    10141 ccagtgcgcc agatttaact ttgtttgtat cgtagacgta gtaactggca gttatcggaa
    10201 ttgaaaccgt tgtatctgta agttttgact ttgccgcaat ttcttttaaa tttgtgtatc
    10261 gtttactgct gttagtgata tttaaaagtg tgactgacga accattactt tcaagaataa
    10321 atcctacgcc attatcttcc gcacctttca gtattgtgtt gctgttggtt aacaagtcag
    10381 aacttgataa atacacctgc attttccgtg atgtttgatt ttgttgacct aataaatttt
    10441 tacaacgcag aataatatcc ccttgtttac tatttattgt tgctttgtta cctgtggctg
    10501 gtaattgtga aacgggaata ctgtctaaag taattaataa atcgtccggt ttgcaggttg
    10561 tggttatcgg gttgtatgac aatgtcagat taaaggaata gacacctgca ttttgtgcca
    10621 atgcatttct gtagctattt acacatgcat cccatgactt gccacctaat aaacacagta
    10681 gaaattgtac tgcggcactc ccgcgtgtcg atgatgaagc actggataat gtcgcaactc
    10741 cattaatcat cactgaaccg ccgccccccg cagaaacatt cacttttgca ttactggccg
    10801 caggtgtcac cgttaccgaa taaccactgg tgaaactggt ctgattattt gataacgtga
    10861 tgttgttttt attggtccat tcaaaatgta aatttaattt aatggtatcg ttataaagtc
    10921 caacaataat atccgacggg ctcatagggg tgatagtatt tgtactcacg catgacgaat
    10981 cattaaaatt atgctcatat gtcgccattt gtgtttgagt aagtgcaaaa tccccctgaa
    11041 caaccaaatt gtgctgaaca gataactcac tataacatgc cgcatagctc acaggcataa
    11101 ccatcatcat tactataaat aacattatac gaatattcat aaccacctga ctattactgg
    11161 caaatatagt ttctactttc atcaatctct ttaccgaagg tgattgtgca tgaaagtcct
    11221 tgttgcttat caattgctac attaaccgat ggcggtactt cattggtgcg aataaataac
    11281 tggcttccct gaccgacaac gccaatatta tgaccatgga tatcattgac ttcataacca
    11341 aacattaatg gttgcccatc cgctcttaac gcttttataa accagggctt gcgctgatcg
    11401 gtatcaaaat taaccagtac aaccgcgccg cgataagggg cggcaatttt ccggttgcca
    11461 cgtaattctg cttcgctatc gctttgcgac acatccagca tcaggtgatt ttcccgataa
    11521 ggtgtcattc cgtcgtatac caccactcca ttacggtttg ttgtgcgata tttttgccca
    11581 ttgacataag catctttaat tcctggcgca ttcatcacag caaacgtttc ggaaagacgg
    11641 ttcgccagat taacgccccc cgaccaggcg acaatgcccc ctgaaacact ggctccagcc
    11701 tgtcgataag tactcgactg actataactg ccattcactg tcgcaaccgg cgcgttccag
    11761 gtcaaattcg ccccagctgt cgtttcattt ccctgattct gataactcag gttgacccca
    11821 tagttaaact gatcccggct tccaacggtt cctgataatc ccgtattatt tgaggcaaac
    11881 ccctgatcat caaacgtcgt tgagttagac atatatattt gccgacgagg cgtcgtaacg
    11941 tcatcacccc aatcaaaggg aatcgatata aaaatattaa aacgtttctc ttcatgatga
    12001 ttctcgtcat acgcctggct tgccgcgagg atatagctta tccgccgcca gttattggaa
    12061 taactcaact gataatcctt actgctgccg ctacgccccc agtaatctcg ccataacgta
    12121 cttaaggaca cagaccccca gccttctggc aatgactggc tcatattggc agagaagcta
    12181 tttttacggc caaaatcgtt ctggtaataa tcggcaatgt catagacatc gttttcatca
    12241 cggcgataat tatctttatt gtttgcccaa acgtgatcat taaatgtccg gtaatcacgc
    12301 gatgaataac gccaggccgc cagaccaaaa cgcgtcgacg tttggctcac aaatttgttg
    12361 taggcaattt gataactttg cccgtcaaac acatcgccgt tgtcttgttt actatgcgac
    12421 ttcgtggcat cgacggaaat ggcaccaatg cgtgtattcc agccagtccc caaagtaaac
    12481 gcgtaataat tattcgcgac catcgagcca ccatacagcg tcaataaatt attaaaacca
    12541 tactgataac ccgcctggac aaaatcactt tgtttgctcg ccccttcaat atggctacga
    12601 cccgccgcaa aatcatattt cgatacgccg ggttgcagca tatttggcac tgctgcataa
    12661 ggcaccagat aggtggttac cgagccgtcc gcctctttca cgctgacatc aagatctgct
    12721 ccaccaccgg ccaactgcaa atctgtaatc gcgaacgggc caggaggaac ctctttctga
    12781 taaaccacaa aaccgttctg ttcaatagtt accagcgcat tactctgagc aatcccctgc
    12841 acccgtggcg taaaattttg tttcgagtta ggcaacatct gcatatcacg aaacaaccgc
    12901 acaccgctga agcgaacaga atcaaaaata tcgcttgatg tgtacatatc acccacgcga
    12961 agcgtgccga gaagttgggc aaatccacgt tccagataca gggtattgct tttccacccc
    13021 cctggattgt tatttgtttt actgaaactg gcatcagaat gcagttgcca ccccagtaaa
    13081 tttaacccgc tgttaaaacg tacatatgta ctcttactat tacccgacgc tttatagtca
    13141 ctgtaatact gactcacata ataagaggta taaaacgcat taataccccg ctcccagttt
    13201 tccggtggaa catagccact ttccagttct tccacccagg cctgcgggac actgaaatcg
    13261 agacgaaaaa ccccgatatc ccaggtatag ctcccaccct gaacaagttg ctcaaatgtt
    13321 aaacattgct taccgctggc gaagttatcg ctattaatgc ctaaccgctt gataacttct
    13381 attgataaac atgtttcttg cgggttgtct ttaacaataa tctcatattt cccgcgccat
    13441 tgcttattga cataaatatc gatgtcatac tgccccggta agggttgatt atcatcaaga
    13501 cgaatatttg caacctgctg gtctttcatt ccacctatca taaaatgggt atcaaaggtt
    13561 tcttcagctg cataagcttc aatgccgagc aataacgcta cgattgctga tgcaagtggg
    13621 gtcattctca acataggcag ctcctgcaat taaattttgt cactaatata gttgccgtgg
    13681 tcatcgataa tggtcagata ccagttattt gcattattac ttttgacatt aacactctga
    13741 ctttctaagg gggcaatcat aatagtttca taattgactt taacattatt agctttgaca
    13801 tccgaaatcg tcacccaatt agctgagtca ttttttatca ccaatccatt gccactgcga
    13861 tttaccagta actttttaaa ggtcgcttta tttaccggag caatacccgc tggccggtaa
    13921 aacaacttaa ttctgttttg catcgcaaac ttcagtgcat ttttgccttc ttgctctgga
    13981 ctatttggtg gaatatccag aacattcaga taaaaaatgc tttctttatt agtgggcagt
    14041 ttattcggca taattttgat ttttacttgc tgcccggaat ttgcccctat ttttgccact
    14101 ggtggcgtta acatgaaagg aacctgaatt ttttctggtg gtaacgacgt atcgccatca
    14161 tcaatccacg cctgcagcag cgaagaacgg tttccctggt tcatcaactg caccataact
    14221 tctttatttt ctgccgggta aataatgcgc gtcccgtaga taacaattcc ggcatgtgca
    14281 ggaaggacca tagaaaaaat gagtaaagat aataatcctt tcatcttaat gcctcttaaa
    14341 ttaaaaagcc gccatgggga tagcggccat gtttattacc tggagaaact tacaatttta
    14401 tttattaatc ctgcgccaca cgcataacca gagaagataa cacatcacct gattgcactg
    14461 agttatctgt cagtttcagg tattttgctt taaacggaat agcatattga ccttccgctg
    14521 ttttataact ctcaatgtta aaaggtgcag aaacatccag gacatcgctg ttatcaccta
    14581 aatagagata gtaacccacg gtatcgttgc tactggctat cgccttatcg ttaccggtta
    14641 ttttatcgtt ggtaatcaac gagagtttta caccagcaac ttctttcggg cagctaattt
    14701 ttaacatgct gctggttaca ccgactgcat catcataggt agaatattta gctttacctt
    14761 caaagacagt ggttggtaca gtcggcatat taatacttac tgaatcacca cccgcttcga
    14821 atttacacgg agattctgca atattcccct taatagttaa tgttccggta tcaacgtctg
    14881 cagcaaatac tccagcgctc ataaaaaaga agaaaagaca gcggcag
//
LOCUS       DAZGTR010000106        14906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGTR010000106 DAZGTR010000000
VERSION     DAZGTR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.108"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            115..356
                     /gene="yjbE"
                     /locus_tag="ACHLCY_003653"
                     /pseudo
     CDS             115..356
                     /gene="yjbE"
                     /locus_tag="ACHLCY_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
     gene            470..1108
                     /locus_tag="ACHLCY_003654"
     CDS             470..1108
                     /locus_tag="ACHLCY_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP0370831.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWGGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1105..1842
                     /locus_tag="ACHLCY_003655"
     CDS             1105..1842
                     /locus_tag="ACHLCY_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0370832.1"
                     /translation="MIKQTFVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1842..3938
                     /locus_tag="ACHLCY_003656"
     CDS             1842..3938
                     /locus_tag="ACHLCY_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0370833.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKVAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3985..4263)
                     /locus_tag="ACHLCY_003657"
     CDS             complement(3985..4263)
                     /locus_tag="ACHLCY_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370834.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4264..4407
                     /locus_tag="ACHLCY_003658"
     CDS             4264..4407
                     /locus_tag="ACHLCY_003658"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370835.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4533..4943
                     /gene="psiE"
                     /locus_tag="ACHLCY_003659"
     CDS             4533..4943
                     /gene="psiE"
                     /locus_tag="ACHLCY_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP0370836.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4987..6462)
                     /gene="xylE"
                     /locus_tag="ACHLCY_003660"
     CDS             complement(4987..6462)
                     /gene="xylE"
                     /locus_tag="ACHLCY_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP0370837.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLHTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVGCVNYFIARSGDATWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNALAAQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVTHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6834..7724)
                     /gene="malG"
                     /locus_tag="ACHLCY_003661"
     CDS             complement(6834..7724)
                     /gene="malG"
                     /locus_tag="ACHLCY_003661"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP0370838.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7739..9283)
                     /gene="malF"
                     /locus_tag="ACHLCY_003662"
     CDS             complement(7739..9283)
                     /gene="malF"
                     /locus_tag="ACHLCY_003662"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP0370839.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9437..10627)
                     /gene="malE"
                     /locus_tag="ACHLCY_003663"
     CDS             complement(9437..10627)
                     /gene="malE"
                     /locus_tag="ACHLCY_003663"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP0370840.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10992..12107
                     /gene="malK"
                     /locus_tag="ACHLCY_003664"
     CDS             10992..12107
                     /gene="malK"
                     /locus_tag="ACHLCY_003664"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP0370841.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12179..13519
                     /gene="lamB"
                     /locus_tag="ACHLCY_003665"
     CDS             12179..13519
                     /gene="lamB"
                     /locus_tag="ACHLCY_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP0370842.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13762..14682
                     /gene="malM"
                     /locus_tag="ACHLCY_003666"
     CDS             13762..14682
                     /gene="malM"
                     /locus_tag="ACHLCY_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP0370843.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3685 a         3848 c         3930 g         3443 t
ORIGIN      
        1 aaacgccatc gctaattccc cgcgccgtaa ttcgcatgct ttagttgtgt atactcgatc
       61 ccgcccgaaa tgtttttggg taaatctcca ttcattcaat gaagggaaat tgttatgaaa
      121 aaagttctgt atggcatttt tgccatatct gcgcttgcgg cgacttctgc gtgggctgca
      181 cctgtacagg tggcgaagcg gcagggtcgg cagcaacgtc ggtttcggcg gggagttcct
      241 ccgctaccat cgtcagcacc gtaagctcgg cggtgggtgt cgcgctcgcg gcaaccggtg
      301 gcggtgatgg ttctaatacc gggaccacca caaccacgac caccagtacc cagtaataaa
      361 gtatgtatcc ccaaaataat ttgagtcatt gcatctgtgg cttgaagtat gaagggattt
      421 accataacca cactccggtg tggttattct gcccctctgg agaagagtcg tgaagcgacc
      481 tgcactcatt cttatctgcc tgctattaca ggcctgttca gccacgacta aagagctggg
      541 caattcactg tgggacagtc tgttcggcac gccaggcgta cagctgacgg acgatgatat
      601 tcaaaatatg ccctacgcca gccagtacat gcagcttaat ggcgggccgc aattgtttgt
      661 ggtgctggcc ttcgctgaag acggacaaca aaaatgggtc actcaggatc aagccactct
      721 tgtcacacaa catggtcgtc tggtgaagac tttgcttggc ggcgacaacc tgattgaagt
      781 gaataacctt gccgccgacc cgctgattaa acccgcacaa attgttgatg gtgcaacatg
      841 gacgcgcacg atgggctgga ccgagtacca gcaggtacgc tacgccaccg cacgctcagt
      901 cttcaaatgg ggtggcaccg ataccgtcaa agtcggcagc gatgaaaccc tggttcgcgt
      961 gctggacgaa gaagtctcca ccgaccaggc gcgctggcat aaccgctact ggatcgacag
     1021 cgaagggcaa attcgccagt cggaacagta tctcggcgcg gattatttcc cggtgaaaac
     1081 cactctcatc aaggcggcaa aacaatgatt aaacaaactt ttgtcgcgtt gcttttgagt
     1141 gtgggagcgt catcggtctt cgcggcagga accgtcaagg tgttcagcaa tggcagcagt
     1201 gaggccaaaa cactaacagg cgcagagcat ttaatcgatc tggtaggcca accgcggctg
     1261 gcaaacagct ggtggcccgg tgcggtgatt agcgaagagc tggcaacggc ggcagcattg
     1321 cgtcagcagc aggcgttgct gacacggctg gcagaacagg gcgcagattc cagcaccgac
     1381 gatgccgccg ccattaacgc cttacgccag caaatccagg cattgaaagt gacaggcagg
     1441 caaaaaatca atcttgatcc cgatatcgtc cgcgttgccg aacgcggtaa cccgccgttg
     1501 cagggcaact acacgctgtg ggtcggacca ccgccgtcca cggtcacgtt gttcgggctt
     1561 atcagccatc ctggcaatca gccattcact ccaggtcgcg acgtggcgag ctatctctcc
     1621 gggcagaacc tgcttagcgg tgcggatcgc agctacgcgt gggtcgttta cccggacgga
     1681 cgcacgcaaa aagcgccggt ggcttactgg aacaagcgtc acgtagagcc gatgcccggc
     1741 agcattattt atgttggcct cgcggactcc gtctggagtg agacgcctga tgcccttaac
     1801 gccgacattc ttcagactct gacgcagcgg ataccccaat aatgaaaaaa agacatctgc
     1861 ttagcttact ggcgctgggc attagcatgg cttgctacgg cgaaacatat cctgcgccca
     1921 ttggcccgtc gcagtcggat ttcggtggcg taggattatt acaaactccc accgcgcgta
     1981 tggcacggga aggggagttg agtctgaact atcgcgataa cgatcagtac cgttattact
     2041 cagcttcagt gcaactcttc ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca
     2101 cccggcagta cagcagcgtc gaagcgttct ctggcgatca aacgtataaa gataaagcct
     2161 tcgatctcaa actgcgtttg tgggaagaga gttattggct gccgcaagtg gcggttggtg
     2221 cgcgggatat cggcggtacg gggctgtttg atgcggaata tcttgttgcc agcaaagcct
     2281 gggggccgtt cgattttacg ctcggcctgg gctggggata tctgggcacc agcggtaatg
     2341 tgaaaaatcc gctctgttca gccagtgata aatattgcta tcgcgataac agctacaaac
     2401 aggcgggatc tatcgacggt agccagatgt tccacggtcc tgcctcactg tttggcggcg
     2461 tggaatacca gacgccctgg caaccgctgc gcctgaaact ggagtatgaa ggcaataatt
     2521 atcagcagga ttttgccggg aagctggagc aaaaaagtaa gtttaacgtc ggtgcgattt
     2581 atcgcgttac cgattgggcc gacgttaacc ttagctatga acgtggcaac acctttatgt
     2641 ttggcgttac gttgcgcacc aactttaacg atctgcgccc gtcttacaac gataacgccc
     2701 gcccgcaata tcaaccgcag ccgcaggatg ccattttgca gcattcggtg gtggcgaatc
     2761 agttaacgtt gctgaaatac aatgccggac ttgccgatcc gcagatccag gcgaaaggcg
     2821 atacgctgta tgttaccggc gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc
     2881 gtgcgaatcg gatcgtgatg aacgatctgc cggatgggat caaaacgatc cgcattacag
     2941 aaaatcgcct taacatgccg caggtgacga cggaaaccga tgtcgccagc ttgaaaaatc
     3001 atctcgccgg ggagccgctg ggccatgaaa cgaaggtggc gcaaaaacgc gtcgagccag
     3061 tggttccgaa gtccaccgag cagggatggt atatcgacaa atcacgcttt gatttccata
     3121 tcgatccggt gctgaaccag tcggtcggtg gcccggaaaa cttttacatg tatcagctgg
     3181 gcgtgatggg aacggcagat ttgtggctga cggaccatct gctgaccacc ggcagcctgt
     3241 ttgcaaatct tgccaacaac tacgacaagt ttaactacac caatccgccg caggactcgc
     3301 acttaccgcg cgttcgtacc catgtgcgcg agtatgtgca gaacgatgtc tatgtgaata
     3361 acctgcaagc caactacttc cagcatctgg gcaacggctt ctacggtcag gtctatggag
     3421 gttatctcga aaccatgttt ggcggtgccg gggcagaagt gttgtatcgt ccgctggaca
     3481 gcaactgggc gtttggtctg gatgccaact acgttaaaca gcgcgactgg cgtagtgcaa
     3541 aagatatgat gaaattcacc gactacagcg tgaaaaccgg acatctgacc gcctactgga
     3601 cgccatcttt cgcccaggac gtgttggtga aagcgagcgt cgggcagtat ctggcagggg
     3661 ataaaggcgg cacgctggag atcgccaaac gctttgatag cggcgtggtg gttggcggct
     3721 atgccacgat cacgaatgtt tcgaaagagg agtacggcga aggggacttc accaaaggcg
     3781 tgtatgtctc tgtgccgttg gatctcttct cgtctggccc gacacgcagc cgtgcggcga
     3841 ttggctggac gccgctgacg cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt
     3901 atgacatgac cagcgaccgt agcgtcaatt tccgctaagt catgggaaag gtgccagttt
     3961 tcgcaatcac tggcaccctc attcttaatg gcctgttgag ccatcaggaa cgcagttcac
     4021 ccccggtttg cgatctggtg tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat
     4081 cggttgcaga tctggttgcc tgagcgactg ggccatcatt gggctggaga cattgctttg
     4141 cggtaggtcg gctttatcag cagtaccgag cggaccagca taagcaggaa gaacagagac
     4201 tgataacatc aaagcagcaa aataaggctt catttttacc acctttatca ggttacgttt
     4261 catttgttcc agaggaacat tgtcgatttt tcgcgcattg ctggtggctg ggaatcacct
     4321 gaatgggtga tttttgaatt accggctttg gtgcggtttg tcttgccgga tgcgccgcca
     4381 ggcgcggctt atccggccta cgggtaggta tatccggctt tggtggaggc gcgctccaaa
     4441 tccaggttga acaaaacata cacaaaaaat atagatctcc gtcacatttt tgcgttatac
     4501 aggaagctcg ccactgtgaa ggaggtactg ctatgacgtc actctctcgt ccgcgcgtgg
     4561 agtttatctc caccattttg cagaccgtac tcaatcttgg cctgttgtgc ctcggcctga
     4621 ttttggttgt cttcctcggc aaagaaacgg tgcatctggc tgatgtgctg ttcgcgccag
     4681 aacaaaccag caaatatgag ctggtagaag ggctggtggt ttactttctc tatttcgaat
     4741 ttatcgcgct gattgtgaag tactttcagt ccggttttca cttcccgcta cgttactttg
     4801 tctatattgg gatcaccgcc attgtgcgct tgatcatcgt cgatcataaa tcgccactgg
     4861 atgtattgat ctactcggca gcgatcctgt tactggtgat caccctgtgg ctgtgtaatt
     4921 cgaaacggct gaagcgggag taaaaagaca gcacgccgaa ctggcgcggc gtgctggaca
     4981 ggaagattac agtgtagcag tttgttgtgt tttcttcgtt tccggttccc agagggcttc
     5041 cagctcctca agggttttac ctttggtttc cgggacaaat ttccacataa atagtgctgc
     5101 cagaacgccc atacaaccgt aaatccagta ggagaaaccg ttgtggaaat gggtcaccag
     5161 ccaggagttt ttgtccatca tcgggaaggt ccaggagacg aagtagttcg ccagccactg
     5221 ggccgccacc gcgattgcca gcgctttacc acgaatagca ttcgggaaga tttccgacag
     5281 cagtacccag cataccggac cccaggacat ggcaaaggca gcaacataga acagcatcga
     5341 cagtagcgcc acaatacccg gtgcctgagt gtaaaacgcg gtaccgaggc taaacatacc
     5401 gattgccatt ccgagcgcgc cgataatttg cagtggctta cgaccaaatt tatccaccgt
     5461 cataattgcc agaacggtga aggtgaggtt gataactccg acaataatgg tctgcaacag
     5521 cgcgatatcc gtgctggccc ccagcgtttt gaacacttcc ggcgcgtagt acaacaccac
     5581 attgatgccg acaaattgct ggaagatgga gagcataacg ccgataacaa tcacgcccac
     5641 gccgaacatc agcagacgac caccggtttt gcgtccatga tccagggagt gtttaatttc
     5701 ctgcaccgcc tgggctgcca aagcgttgcc cataattttg cgcaggatgc cttccgcttg
     5761 ttcttgctta ccgcgtgaca tcagccagcg agggctttcc ggcacggtat aaagcagcat
     5821 taagaacagc agcgcaggga tacattccga agcaaacata taacgccagc cgtcagtatt
     5881 cagccaggtg gcatcgccgg aacgggcaat aaaatagttt acgcagccaa ctaaaagttg
     5941 gccgaaaata atcgcaaact ggttaaaaga gaccagcttc ccacgaatat gtgcaggagc
     6001 cagttccgca atatacattg gtgagagcat tgaggctaaa ccaacaccaa taccgccaat
     6061 aatgcgataa ataacaaatt ccgggacata acctgccaga taaacaggca ctgtgttgtc
     6121 cgggtttata gaggtaaaac caagttctgg ccaggcagaa cctacaccag aaataaaaaa
     6181 caggacagca gcaatcttaa gtgaatcacg acgaccgaag cggttactgc aataaccacc
     6241 gagggcaccg ccgatgatgc aaccaatcag agcgctggct acgcaaaacc ctaacaggga
     6301 gttggcagcg gattcactta agttttgtgg agcaacaaag acggtatgga gtgactcaac
     6361 agtaccggaa ataacggcgg tgtcgtagcc aaataataaa ccacctaatg tagcgactaa
     6421 ggtaatcgaa aatatataac tggaattata ctgggtattc attcagacct gccttagacc
     6481 attctgatct tttatcagtg cattgatgtt ggaattgtca ggcagttaag gaaattatca
     6541 ttttttacaa tgcgtttacg taatgtcttt tctgtgatct taattgtgat aattatctaa
     6601 aattgatgaa aacaatacta ttgccgtgac tcagagcacg aaagagaatt atcgtaagtg
     6661 ggaaaacaaa taacgtaaaa ataataagct ctatgatggg aatattaacc ggcgaacgat
     6721 tcagattgca gacgaaagaa aaaaaggcgc tccgtggagc gccgaataac agtcacaagt
     6781 tgggataacg taagttgagg gtgcagcggc ataacattgg cagaacaaca tctttaacct
     6841 ttcacaccac ctgccgtcag gccgttgacc agccagcgtt gagccagcaa gaagacgatg
     6901 gtgatcggta atgcagacat cacggcagcg gcggcaaagt caccccacag gtagttttgc
     6961 gggttgaggt attgctgcat ccccacggcc agggtgtagc tgtttacgtc acgcagtaac
     7021 agcgacgcga ccggaacttc agtaatggca gcgataaacg acaagatgaa taccaccgcc
     7081 agaatcggta ctgacagcgg caacaggaca aggcggaagg cctgccacgg tgtcgcacca
     7141 tccagcgcag ccgcttcttc cagcgaacta tcgatggttt cgaaatagcc tttgatggtc
     7201 cagacatgca gcgcaatccc acccagatac gcgaaaatta cgccgccgtg agtattcagg
     7261 ccaatgaatg gaatgtactc acccagacga tcaaacaacg catacaacgc gaccagtgaa
     7321 agtactgccg ggaacatctg gaaaatcagc attcctttca gcagcgtcgc tttgcctgga
     7381 aagcgcatac gggcgaaagc gtaggcgcag gtggtagaga gcgccacaat gccaatcgcg
     7441 gaaatcccgg cgacctttac cgagttccac agccacagca gtaccgggaa tggcggtggc
     7501 gtaatgcgac catcagcctg ttcaacgcta aaacctaacg ccagtttcca gtgatcccag
     7561 gagatttgct ccgggatcag gctaccggtc gcgaagtttc cctgacgcag cgagatagcg
     7621 acgaccatca gcagcgggaa cataatcgcc gcgataaaaa gtagcagtag caggtgagta
     7681 ataaacaaac gtgctttttg cgatttcggt tggaccattg ccatttttgt tatctccctt
     7741 aatcaaactt cattcgcgtg gctttcaggt tcactatcgc cagcgcaccc accagcagga
     7801 agatcagcgt ggcaattgct gctgccagac cgaagtcctg acccccgccg ccttcaaaag
     7861 cgatgcggta ggtgtagtta acaagcaggt cggtataacc ggctggcgtg gtcgtgccaa
     7921 gacgatccgg gccgccgttg gttaacagtt gaatcagcac gaagttgtta aagttaaagg
     7981 cgaagctggc gatcatcagc ggcgtcagcg gtttaatcag cagcggcagc gtaatcttaa
     8041 agaagttctg gaacggacct gcgccatcca ttgctgaggc ttcatacaaa tcgtccggaa
     8101 tcgctttcag caagcccatg cagaggatca tcatgtacgg ataacccagc caggtattga
     8161 cgataatcag catcgtgcgg gcggtggtcg gatcgctgaa ccaggcgggc ttcacgccaa
     8221 acagcgcgct caacatcatg ttgatttcac cgaagctctg gttaaacaac cctttgaaaa
     8281 tcaagattga aatgaacgat ggcaccgcgt agggcagaat cagcaggacg cgatagaccg
     8341 ctttgccgcg caacgcttcc cactgcacca gacacgccag aaccatgccg accgccaccg
     8401 ttaaaaagac agtgatcagc gagaacacca cggtccagac gaaaatggcg aggaacggtt
     8461 tctgaatgcc ttcgtcggtg aagacgcggg taaagttttt ccagccagta gtcacggtgt
     8521 aaccggggct tagcttttca tcgccccagt tgccgtcggc ggtaatggac tggtaaaagc
     8581 caatttggtt attcggacga tatttcacgc cgctctgatt attcgtcaac gtgccgtcac
     8641 cgtcgagtgt gtagagcggc tgcgtgccag aaaactggcg cagggagctc atcatcactt
     8701 tgttgccatc cggcagaatg gcggtaatgt cactcagcgc ctgacgattc tgggtaatca
     8761 cgcgcagatt cgcgcgttcg ccttcgggct gggtggtcgt ttctttcagt tgcagttttt
     8821 gctcgccgcc aaatttaaaa gcgtcggaga ggtaattttt gccggtttcg ccgtcgctga
     8881 gggctagttg ccactcatcg cccgctgggt aaagaccaaa attataggtt ttgcctgctt
     8941 gccaggagcg atctagcaac acttcctgcg cacgttcaaa agtcagctgg ttagtgctgc
     9001 tgtagttggt gaaggcaatg gcgatggtgc agaccagagg gaagaggacg aataatccca
     9061 ttccagccat tcccgggtaa acatagcgcc aggcgtaggc tttacgattg gcgaaaatat
     9121 acagccccgc tgaactcaat atcagcgtgg taatggcgaa caggtattcc ccttgtgcgt
     9181 acattaaaac aacaaggtaa cccaccagca ggccgagcag acctaacact gaccatttca
     9241 gcgcgtcgct ttgccaccaa tgtttctttt taatgacatc catggggttc ttcctcattc
     9301 caggacggat aaggctttca cgccttatcc gacaacaact gcctgatgcg acgctgacgc
     9361 gtcttatcag gcctacatac gtttcggttt tgtaggccgg acaaggcgtt cacgccgcat
     9421 ccggcatttc acagcattac ttggtgatac gagtctgcgc gtctttcagg gcttcatcga
     9481 cagtctgacg accgctggcg gcgttgatca ccgcagtacg cacggcatac cagaaagcgg
     9541 acatctgcgg gatgttcggc atgatttcac ctttctgggc gttttccatg gtggctgcaa
     9601 tacgtggatc tttcgccaac tcttcctcgt aagacttcag cgctacggca cccagcggtt
     9661 tgtctttatt aaccgcttcc agaccttcgt cagtcagcag atagttttcg aggaactctt
     9721 tcgccagctc tttgttcgga ctggcggcgt taataccggc gctcagcacg ccaacgaacg
     9781 gtttagatgg ttgacccttg aaggtcggca gtaccgttac accataattc actttgctgg
     9841 tgtcgatgtt ggaccatgcc cacgggccgt tgatggtcat cgctgtttcg cctttattaa
     9901 aggcagcttc tgcgatggag taatcggtgt ctgcattcat gtgtttgttt ttaatcaggt
     9961 caaccaggaa ggtcagaccc gctttcgcgc cagcgttatc cacgcccacg tctttaatgt
    10021 cgtatttgcc attttcatac ttgaacgcat aacccccgtc agcagcaatc agcggccagg
    10081 tgaagtacgg ttcttgcagg ttgaacatca gcgcgctctt acctttcgct ttcagttctt
    10141 tatccagcgc cgggatctct tcccaggttt ttggtgggtt cggcagcaga tctttgttat
    10201 aaatcagcga taacgcttca acagcgatcg ggtaagcaat cagcttgccg ttgtaacgta
    10261 cggcatccca ggtaaacgga tacagcttgt cctggaacgc tttgtccggg gtgatttcag
    10321 ccaacaggcc agattgagcg tagccaccaa aacggtcgtg cgcccagaag ataatgtcag
    10381 ggccatcgcc agttgccgca acctgaggga atttctcttc cagtttatcc ggatgctcaa
    10441 cggtgacttt aattccggta tctttctcga atttcttacc gacttcagcg agaccgttat
    10501 agcctttatc gccgttaatc cagattacca gtttaccttc ttcgattttg gcgagagccg
    10561 aggcggaaaa catcatcgtc gttaatgcgg ataatgcgag gatgcgtgca cctgttttta
    10621 ttttcataat ctatggtcct tgttggtgaa gtgctcgtga aaacacctaa acggactcta
    10681 gtttctttat acggcaacct ctttccatcc tccttgcccc tacgccccac cgttgctttg
    10741 tgtgatctct gttacagaat tggcggtaat gtggagatgc gcacataaaa tcgccacgat
    10801 ttttgcaagc aacatcacga aattccttac atgacctcgg tttagttcac agaagccgtg
    10861 ttctcatcct cccgcctcct cccccataaa aaagccaggg ggtggaggat ttaagccatc
    10921 tcctgatgac gcatagtcag cccatcatga atgttggtgt cgatgacagg ttgttacaaa
    10981 gggagaaggg catggcgagc gtacagctgc aaaatgtaac gaaagcctgg ggcgaggtcg
    11041 tggtatcgaa agatatcaat ctcgatatcc atgaaggtga attcgtggtg tttgtcggac
    11101 cgtctggctg cggtaaatcg actttactgc gcatgattgc cgggcttgag acgatcacca
    11161 gcggcgacct gttcatcggt gagaaacgga tgaatgacac tccgccagca gaacgcggcg
    11221 ttggtatggt gtttcagtct tacgcgctct atccccacct gtcagtagca gaaaacatgt
    11281 catttggcct gaaactggct ggcgcaaaaa aagaggtgat taaccaacgc gttaaccagg
    11341 tggcggaagt gctgcaactg gcgcatttgc tggatcgcaa accgaaagcg ctctctggcg
    11401 gtcagcgtca gcgtgtggcg attggccgta cgctggtggc cgagccaagc gtatttttgc
    11461 tcgatgaacc gctctccaac ctcgatgctg cactgcgtgt gcaaatgcgt atcgaaatct
    11521 cccgtctgca taaacgcctg ggccgcacaa tgatttacgt cacccacgat caggtcgaag
    11581 cgatgacgct ggccgacaaa atcgtggtgc tggacgcagg tcgcgtggcg caggttggga
    11641 aaccgctgga gctgtaccac tatccggcag accgttttgt cgccggattt atcggttcgc
    11701 caaagatgaa cttcctgccg gtaaaagtga ccgccaccgc aatcgatcaa gtgcaggtgg
    11761 agctgccgat gccaaatcgt cagcaagtct ggctgccagt tgaaagccgt gatgtccagg
    11821 ttggagccaa tatgtcgctg ggtattcgcc cggaacatct actgccgagt gatatcgctg
    11881 acgtcatcct tgagggtgaa gttcaggtcg tcgagcaact cggcaacgaa acccaaatcc
    11941 atatccagat cccttccatt cgtcaaaacc tggtgtaccg ccagaacgac gtggtgttgg
    12001 tagaagaagg tgccacattc gctatcggcc tgccgccaga gcgttgccat ctgttccgtg
    12061 aggatggcac tgcatgtcgt cgactgcata aggagccggg cgtttaagca ccccacaaaa
    12121 cacacaaagc ctgtcacagg tgatgtgaaa aaagaaaagc aatgactcag gagatagaat
    12181 gatgattact ctgcgcaaac ttcctctggc ggttgccgtc gcagcgggcg taatgtctgc
    12241 tcaggcaatg gctgttgatt tccacggcta tgcacgttcc ggtattggct ggacaggtag
    12301 cggcggtgaa caacagtgtt tccagactac cggtgctcaa agtaaatacc gtcttggcaa
    12361 cgaatgtgaa acttatgctg aattaaaatt gggtcaggaa gtgtggaaag agggcgataa
    12421 gagcttctat ttcgacacta acgtggccta ttccgtcgcg caacagaatg actgggaagc
    12481 taccgatccg gccttccgtg aagcaaacgt gcagggtaaa aacctgatcg aatggctgcc
    12541 aggttccacc atctgggcag gtaagcgctt ctaccaacgt catgacgttc atatgatcga
    12601 cttctactac tgggatattt ctggtcctgg tgccggtctg gaaaacatcg atgttggctt
    12661 cggtaaactc tctctggcag caacccgctc ctctgaagct ggtggttctt cctcttttgc
    12721 cagcaacaat atttatgact ataccaacga aaccgcgaac gacgttttcg atgtgcgttt
    12781 agcgcagatg gaaatcaacc cgggcggcac attagaactg ggtgtcgact acggtcgtgc
    12841 caacctgcgt gataactatc gtctggttga tggcgcatcg aaagacggct ggttattcac
    12901 tgctgaacat actcagagtg tcctgaaggg ctttaacaag tttgttgttc agtacgctac
    12961 tgactcgatg acctcgcagg gtaaaggtct gtcgcagggt tctggcgtcg cgtttgataa
    13021 cgaaaaattt gcctacaata tcaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg
    13081 tgcgatctcc atgggcgaca actgggacat gatgtacgtg ggtatgtacc aggatatcaa
    13141 ctgggataac gacaacggca ccaagtggtg gaccgttggt attcgcccga tgtacaagtg
    13201 gacgccaatc atgagcaccg tgatggaaat cggctacgac aacgtcgaat cccagcgcac
    13261 cggcgacaag aacaatcagt acaaaattac cctcgcacaa caatggcagg ctggcgacag
    13321 catctggtca cgcccggcta ttcgtgtctt cgcaacctac gccaagtggg atgagaaatg
    13381 gggttatgac tacaacggcg atagcaaggt taacccgaac tacggcaaag ccgttcctgc
    13441 tgatttcaac ggcggcagct tcggtcgtgg cgacagcgac gagtggacct tcggtgccca
    13501 gatggaaatc tggtggtaat agcaaaacct gggccggata aggcgtttac gccgcattcg
    13561 gcaaccaacg cctgatgcga cgcttgcgcg tcttatcagg ccgacaacgg ctgtcaaatg
    13621 taggccggat aaggcgttta cgccgcatcc ggcataaaaa caggttgtca ttatctgaaa
    13681 ggggcgaaag cccctctgat tatcgggttt agcgcgctat tgcctggcta ccgctgagct
    13741 ccagattttg aggtgaaaac aatgaaaatg aataaaagtc tcatcgccct ctgtttatca
    13801 gcagggttac tggcaagcgc gcctggaatc agccttgccg atgttaacta cgtaccgcaa
    13861 aacaccagcg acgcgccagc cattccatct gctgcgctgc aacaactcac ctggacaccg
    13921 gtcgatcaat ctaaaaccca gaccacccaa ctggcgaccg gcggccaaca actgaacgtt
    13981 cccggcatca gtggtccggt tgctgcgtac agcgtcccgg caaacattgg cgaactgacc
    14041 ctgacgctga ccagcgaagt gaacaaacaa accagcgttt ttgcgccgaa cgtgctgatt
    14101 cttgatcaga acatgacccc atcagccttc ttccccagca gttatttcac ctaccaggaa
    14161 ccaggcgtga tgagtgcaga tcggctggaa ggcgttatgc gcctgacacc ggcgttgggg
    14221 cagcaaaaac tttatgttct ggtctttacc acggaaaaag atctccagca gacgacccaa
    14281 ctgctcgacc cggctaaagc ctatgccaag ggcgtcggta actcgatccc ggatatcccc
    14341 gatccggttg ctcgtcatac caccgatggc ttactgaaac tgaaagtgaa aacgaactcc
    14401 agctccagcg tgttggtagg acctttattt ggttcttccg ctccagctcc ggttacggta
    14461 ggtaacacgg cggcaccagc tgtggctgca cccgctccgg caccggtgaa gaaaagcgag
    14521 ccgatgctca acgacacgga aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa
    14581 ggtgatgttg ataaggcgtt aaaactgctt gatgaagctg aacgcctggg atcgacatct
    14641 gcccgttcca cctttatcag cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg
    14701 attttgcaac aactggtgcg tctcctggcg cacctttttt tatgcttcct tcctgggata
    14761 tgagcgattt tttatagtaa ctcacttctt cttcactaag aatatccatt atctcaatgc
    14821 cttatcagag attcttttcc tttcgccggt agtgtctgga cattcaggct acttttccag
    14881 gttattttat ttctgttatg cagagg
//
LOCUS       DAZGTR010000107        14891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGTR010000107 DAZGTR010000000
VERSION     DAZGTR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.109"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..292)
                     /locus_tag="ACHLCY_003667"
     CDS             complement(<1..292)
                     /locus_tag="ACHLCY_003667"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0370844.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFA"
     gene            491..1189
                     /locus_tag="ACHLCY_003668"
     CDS             491..1189
                     /locus_tag="ACHLCY_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0370845.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGRYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1234..2133)
                     /gene="eamA"
                     /locus_tag="ACHLCY_003669"
     CDS             complement(1234..2133)
                     /gene="eamA"
                     /locus_tag="ACHLCY_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP0370846.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2328..3515
                     /gene="ydeE"
                     /locus_tag="ACHLCY_003670"
     CDS             2328..3515
                     /gene="ydeE"
                     /locus_tag="ACHLCY_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP0370847.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFTDNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGHRLNPVNIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3642..3737
                     /gene="mgtS"
                     /locus_tag="ACHLCY_003671"
     CDS             3642..3737
                     /gene="mgtS"
                     /locus_tag="ACHLCY_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP0370848.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3737..3841
                     /gene="mgtT"
                     /locus_tag="ACHLCY_003672"
     CDS             3737..3841
                     /gene="mgtT"
                     /locus_tag="ACHLCY_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP0370849.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3956..4846)
                     /gene="dgcZ"
                     /locus_tag="ACHLCY_003673"
     CDS             complement(3956..4846)
                     /gene="dgcZ"
                     /locus_tag="ACHLCY_003673"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP0370850.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGVLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5101..5493)
                     /gene="ydeI"
                     /locus_tag="ACHLCY_003674"
     CDS             complement(5101..5493)
                     /gene="ydeI"
                     /locus_tag="ACHLCY_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP0370851.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            5769..6287
                     /gene="ydeJ"
                     /locus_tag="ACHLCY_003675"
     CDS             5769..6287
                     /gene="ydeJ"
                     /locus_tag="ACHLCY_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP0370852.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(6332..8377)
                     /gene="dcp"
                     /locus_tag="ACHLCY_003676"
     CDS             complement(6332..8377)
                     /gene="dcp"
                     /locus_tag="ACHLCY_003676"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP0370853.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHSVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            8514..9260
                     /gene="ydfG"
                     /locus_tag="ACHLCY_003677"
     CDS             8514..9260
                     /gene="ydfG"
                     /locus_tag="ACHLCY_003677"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP0370854.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            9349..10035
                     /gene="rspR"
                     /locus_tag="ACHLCY_003678"
                     /gene_synonym="ydfH"
     CDS             9349..10035
                     /gene="rspR"
                     /locus_tag="ACHLCY_003678"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP0370855.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            10213..10416
                     /gene="ydfZ"
                     /locus_tag="ACHLCY_003679"
     CDS             10213..10416
                     /gene="ydfZ"
                     /locus_tag="ACHLCY_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP0370856.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEDCEEKLAPLDLIRLGMN"
     gene            complement(10452..11912)
                     /gene="ydfI"
                     /locus_tag="ACHLCY_003680"
     CDS             complement(10452..11912)
                     /gene="ydfI"
                     /locus_tag="ACHLCY_003680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0370857.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPAIGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNLIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(12001..13284)
                     /gene="ydfJ"
                     /locus_tag="ACHLCY_003681"
                     /pseudo
     CDS             complement(12001..13284)
                     /gene="ydfJ"
                     /locus_tag="ACHLCY_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            13416..13658
                     /locus_tag="ACHLCY_003682"
     CDS             13416..13658
                     /locus_tag="ACHLCY_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0370858.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTRESADEWFVR"
     gene            complement(13786..13925)
                     /locus_tag="ACHLCY_003683"
                     /pseudo
     CDS             complement(13786..13925)
                     /locus_tag="ACHLCY_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            14254..>14891
                     /gene="nleG7"
                     /locus_tag="ACHLCY_003684"
     CDS             14254..>14891
                     /gene="nleG7"
                     /locus_tag="ACHLCY_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFP0370859.1"
                     /translation="MPIFLNFTAGSILPENELASLRYIVQQNQNDTVIIKERYKMDIR
                     YIESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQISQDFMLQLSSNIG
                     WKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGVFVKNALNSNICTL
                     YDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFIIMDADQQK"
BASE COUNT         4102 a         3630 c         3207 g         3952 t
ORIGIN      
        1 tcgcaaattc aagaactgcc tgtccgttca tggtgccgta gtctttcatc tcgataacgg
       61 ctacaggcat tttcccctgt gcaacggctt caatctctgg tttctgaaag cgaacttgcg
      121 ggccaagtaa aacaacgtca acttcgttat ttttaatttt ccctttagcc tctgcaatcg
      181 ccaatgcgga aatattaact tcaagtgaaa tagcgttagc atgatcaatc atacgtttaa
      241 ccagcatact ggttgacatg cccgcagcgc aaactaacaa tatttttttc atattttccc
      301 ttactggtat ataacagatt acattgttta aatatcatat cccgcagtag ctatacgtcc
      361 tggtacattg atcacagtaa tacagtgata aagttgttca acatcagggt gattattaaa
      421 cgactcggcc atacgataaa ccaatcagga ctcaactcct tgttttcaat ggctcttaaa
      481 aggtgcaaaa gtgatttttc aaaagattgc ccgcttactt aaatccgaaa tcaatggcaa
      541 ttcatggcat gttggtgatt tgttgccgtc agaagcggaa ctcgctgttc gttataatgt
      601 ttcgcgtaat accctgcgta aggcattatc cttgctggaa ggcgaaggaa ttattcacag
      661 aaagcatggt tcaggaagat acattcaaaa aaagaatttt gttgcacaca ttgatcacat
      721 gaacagtttc agtgaaattg cgcataaaag cggcaaagag gcagggagtc agattatgaa
      781 atttgaagtg caggatgctt cacccactat tgcgactgag ctgaatttag tgactggtga
      841 gcaggtttat tacataaaga gactgcgatt cattgaggat aatgcagcac aattagaaga
      901 aacgtggatg tcagtagcac gttttcctga tttaaccgtg tcacatatgc aaaaatccaa
      961 attttcatat attgagaatg aatgcgggat caaaatcatt ggcacctttg aaactttctc
     1021 ccctactttt cctaccccag aaatcgccag tattttacgg atcagcccac gggatcccat
     1081 acttaaaatt cagacccagg ctgtggacag taactctatt ccgctggatt attcgttact
     1141 ttacagcaat attttcgagt tccaggtaaa gtactttttc ccgcgataaa agcagacgca
     1201 cagagtgctg acatcacatt ctgtgcgtat ggtttagcct ctaaccttta ccgctttacg
     1261 ccaccgcaag ccaaatacat tgatatacag cccggtcata atgagcaccg cacctaaaaa
     1321 ttgcagaccc gttaagcgtt catccaacaa tagtgccgca cttgccagtc cgactacggg
     1381 caccagtaac gataacggtg caaccctcca ggtttcatag cgtcccagta acgtccccca
     1441 gatcccataa ccaacaattg tcgccacaaa cgccagatac atcagcgaca agatggtggt
     1501 catatcaata gtaaccagac tgtgaatcat ggatgcggaa ccatcgagaa tcagcgaggc
     1561 aacaaagaag ggaatgattg ggattaaagc gctccagatt accagcgaca tcaccgccgg
     1621 acgcgttgag tgcgacatga tctttttatt gaagatgttg ccacacgccc aactaaatgc
     1681 tgcagccagg gtcaacataa agccgagcat cgccacatgc tgaccgttca gactatcttc
     1741 gattaacacc agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag
     1801 tcgctccccg aaagtaaacg cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac
     1861 cagcgaagcc agtccagcag gcataccaaa gttaatggcg cagaacagaa aggcaaactg
     1921 cgcaaaactg atggttaatc cataccccaa gagcaaattc agtggtactt tcggtcgtgc
     1981 aacaaaaaag atggccggaa aagcgaccag cataaagcgc aaaccggcca gcatcagcgg
     2041 tggcatgtta tgaagcccca ctttgatgac cacaaaattt agcccccata cgaccactac
     2101 cagtagcgcc aacaccccat cttttcgcga cattctaccg cctctgaatt tcatcttttg
     2161 taagcaatca acttagctga atttactttt ctttaacagt tgattcgtta gtcgccggtt
     2221 acgacggcat taatgcgcaa ataagtcgct atacttcgga tttttgccat gctatttctt
     2281 tacatctcta aaacaaaaca taacgaaacg cactgccgga cagacaaatg aacttatccc
     2341 tacgacgctc taccagcgcc cttcttgcct cgtcgttgtt attaaccatc ggacgcggcg
     2401 ctacgctgcc atttatgacc atttacttga gtcgccagta cagcctgagt gtcgatctaa
     2461 tcggttatgc gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta
     2521 tcctggcgga taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcca
     2581 gcggttttat tgccattcct ttagtgaata acgtgacgct ggtggtgctc ttttttgccc
     2641 ttattaactg cgcctattcc gtttttgcta ccgtgttgaa agcctggttt accgacaatc
     2701 tttcgtccac cagcaaaacg aaaatcttct caatcaacta caccatgcta aacattggct
     2761 ggaccatcgg tccgccgctc ggcacgctgt tggtgatgca gagcatcaat ctgccctttt
     2821 ggctggcagc tatctgttcc gcgtttccca tgcttttcat tcaaatttgg gtaaagcgca
     2881 gcgagaaaat catcgccacg gaaacaggca gtgtctggtc gccgaaagtt ttattacaag
     2941 ataaagcact gttgtggttt acctgctctg gttttctggc ttcctttgtt agcggcgcat
     3001 ttgcttcatg catttcacaa tatgtgatgg tgattgctga tggggatttt gccgaaaagg
     3061 tggtcgcggt tgttcttccg gttaatgctg ccatggtagt tacgttgcaa tattccgtgg
     3121 gccaccgcct taacccggtt aacatccgcg cgctgatgac agcaggcacc ctctgtttcg
     3181 tcatcggtct ggttggtttt attttttccg gcaacagcct gctattgtgg gggatgtcag
     3241 ctgcggtatt tactgtcggt gaaatcattt atgcgccggg cgagtatatg ttgattgacc
     3301 atattgcgcc gccaggaatg aaagccagct atttttctgc ccagtcttta ggctggcttg
     3361 gtgctgcaat taacccatta gtgagtggcg tagtgctaac cagcctgccg ccttcctcgc
     3421 tgtttatcat cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta aaagggattc
     3481 gtgcaaaacc gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataaaaattt
     3541 aattcagcct tcgtttaggt tacctctgct aatatctttc tcattgagat gaaaattaag
     3601 gtaagcgagg aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg
     3661 tttttatggc cgtactggga ataattttat tttctggttt tctggccgcg tatttcagcc
     3721 acaaatggga tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt
     3781 tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg
     3841 aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg
     3901 ataacggatc gttgacacag tagcatcagt tttctcaatg aatgttaaac ggagcttaaa
     3961 ctcggttaat cacattttgt tcgtcaataa acatgcagcg atttcttccg gtttgcttac
     4021 cctcatacat tgcccggtct gctcttccaa tgaccacatc cagaggctct tcaggaaatg
     4081 cgcgactcac acctgctgtc acggtaatgt tgatatgccc ttcagaatgt gtgatggcat
     4141 ggttatcgac taactggcaa attctgacac ctgcacgaca tgcttcttca tcattagccg
     4201 ctttgacaat aatgataaat tcttcgcccc cgtagcgata aaccgtttcg taatcacgcg
     4261 tccaactggc taagtaagtt gccagggtgc gtaatactac atcgccgatt aaatgcccgt
     4321 aggtatcatt aaccaattta aatcggtcaa tatccaacaa cattaaataa agattcagag
     4381 gctcagtgtt gcgtaactga tgatcaaagg attcatcaag aacccgacgc cccggcaatc
     4441 ccgtcaaaac atccatattg ctacggatcg tcagcaaata aattttgtaa tcggttaatg
     4501 ccgcagtaaa agaaagcacc ccctcctgaa aggcgtcgaa atgcgcgtcc tgccagtgat
     4561 tttcaacaat agccagcatt aattcccgac cacagttatg catatgttga tgggcagaat
     4621 ccattagccg aacgtaaggt aattcatcgt tatcgagtgg ccccagatga tcaatccacc
     4681 gaccaaactg gcacagtcca taagaatggt tatccgttat ttctggctta ctggcatctc
     4741 tcgcgaccac gctgtgaaac atactcacca gccactggta gtgggcatcg atagccttat
     4801 tgagatttaa caagatggca tcaatttccg ttgttttctt gatcattgcc actccttttt
     4861 cacagttcct tgtgcgcgct attctaacga gagaaaagca aaattacgtc aatattttca
     4921 tagaaatccg aagttatgag tcatctctga gataacattg tgatttaaaa caaaatcagc
     4981 ggataaaaaa gtgtttaatt ctgtaaatca cctctgcatt atcgtaaata aaaggatgac
     5041 aaatagcata acccaatacc ctaatggccc agtagttcag gccatcaggc taatttattt
     5101 ttatttctgc aaatgagtga cccgaacgac ggccggcgcg cttttcttat ccagactgcc
     5161 gctaatgttg atcatctggt ccggctgaac ttctcgtcca tcaaagacgg ccgcaggaat
     5221 aacgacatta atttcaccgc tcttatcgcg aaaaacgtaa cggtcctctc ctttgtgaga
     5281 aatcaaatta ccgcgtagtg aaaccgaagc gccatcgtgc atgttttttg cgaaatcaac
     5341 ggtcattttt tttgcatcat cggttccgcg atagccatct tctattgcat gaggcggcgg
     5401 tggcgctgca tcctgtttta aaccgccctg gtcatctgcc aacgcataag gcatgacaag
     5461 aaaacttgct aatacaatgg cctgaaattt catactaact ccttaattgc gtttggtttg
     5521 acttattaag tctggttgct atttttataa ttgccaaata agaatactgc caattgttat
     5581 aaggcattta aaatcagccc actagctgtc aaatattcag agaatttaac tcactaaagt
     5641 taagaagatt gaagagtctt aaacatattt tcagaataat cggatttata tgtttgaaaa
     5701 ttattatatt tgacgagcat acagaaaaag caaatcacct ttacatataa tagcgtggac
     5761 aaaaaacagt gaacattaat aaagataaaa ttgtacaact cgcagatacc gacactattg
     5821 aaaacctgac atccgcgttg agtcaaagac ttatcgcgga tcaattacgc ttaactaccg
     5881 ccgaatcatg caccggcggt aagttggcta gcgccctgtg tgcagctgaa gatacaccca
     5941 aattttacgg tgcaggcttt gttactttca ccgatcaggc aaagatgaaa atcctcagcg
     6001 taagccagca atctcttgaa cgatattctg cggtgagtga gaaagtggca gcagaaatgg
     6061 caaccggagc catagagcgt gcggatgctg atgtaagtat tgccattacc ggctacggcg
     6121 gaccggaggg cggtgaagat ggtacgccag cgggtaccgt ctggttcgcg tggcatatta
     6181 aaggccagac ctatactgcg gttatgcatt ttgctggcga ctgcgaaacg gtattagctt
     6241 tagcggtgag gtttgccctc gcccagctgc tgcaattact gctataacca ggctggcctg
     6301 gcgatatctc caggacagcc attggtggtg tttatatgtt caagccacga tgttgcagca
     6361 tcggcataat ctgaggagcc ttaccgcgcc attgtcgata caggcgttcc agatcttcgc
     6421 tgttacctct ggaaaggatc gcctcgcgaa aacgcagccc attttcacgc gttaatccgc
     6481 cctgctcaac aaaccactga taaccatcat cggccaacat ttgtgtccac agataagcgt
     6541 aataacctgc ggcatatccg ccaccaaaaa tatgggcgaa ataactgctg cgatagcgtg
     6601 gcggtatagc aggaagatcc atattttccg ccaccagcgc ccgcaattca aaatcatcga
     6661 catcctgcat tgcttcgttt tcttccaggc aatgccagcg catatcgaga agtgcggcgc
     6721 taagcagttc gctcatctca taccctttgt tgaacaggct ggcattacgc attttctgtt
     6781 gcagttcgtc aggcattgct gccccgctct gataatgccg ggcgtagcga gcgaatacct
     6841 gcggatgcgt tgcccagtgt tcgttgattt gcgacggaaa ttcgacaaaa tcacgcggcg
     6901 tgttggtgcc ggaaagcgtg gcataacgct ggcgggcaaa aaggccgtgc agcgtatgac
     6961 caaattcatg gaataaggtt atgacatcat cccagagtaa caacgcaggc tcaccggcag
     7021 cgggtttctg ataattgcaa acgttataaa ttaccgaatg cgtttcatta agcgttgatt
     7081 gctcaacaaa attgcccatc catgcaccgc cgctttttga atcacgggcg aagaaatcac
     7141 cgtaaaataa cgccagtccc acgccattat gatcaaaaat ttcccacaca cgaacgtcag
     7201 gatggtagac aggaatatca aaacgttcga caaacttaat accgaagagc tgattcgcgg
     7261 tccagaatac accttcattc aacaccgtgt ttaattcaaa atatggcttg agctgcgcct
     7321 catcaagatc aaatttctcc cgccgtacct gttcggcata aaatgcccag tcccacggct
     7381 gcgcgctaaa cccgccctgc tgcttatcaa taaccgcctg tatggaggct aattcatcgc
     7441 tcgcacgttg acgcgccgct ggaacaattt cccgcataaa gttgagcgct gcttcaggtg
     7501 tttttgccat ctgatcggcg attttccatg cggcataatg aggaaaacca agtaatgtcg
     7561 cctgttgtgc acggatctcc accagacgtt gaatgatagc gcgggtatca ttgccatcat
     7621 ttttttccgc tcgcgtccag cccgcaataa acagtttttc acgcgtcgca cgatcgcgca
     7681 tttcggcaag cgccggttgt tgggtggtat tcagcagcgg aatcagccat ttgttatcca
     7741 gacctttctc gcgagccgcc tctgccgcca gcgcaatctc ttgctcactc attcctgcca
     7801 gctgcgcgat atcgttcaca accagaccgc cggatttatt tgctgccagt aaccgctggt
     7861 taaactgact ggtcagtgtc gcagcttctg tattcagtac ttttaatttt gctttatcag
     7921 cttgcgcaag tttggctccg gcaaggacaa aacgttgatg aatcacctcc accaggcgga
     7981 tggattcact atcaagcccc agggattcac ggcgctgcca gacagcatct acccgcgcga
     8041 ataattcacc gttcagatag atatcattag ccagttccgc cagttcagcg gaaaactgtt
     8101 catcaagacg ctgtaattca tcattggtat gcgccgcagt catcgcaaaa aagacgctgg
     8161 taacgcgggt aagtaattct ccgctttgtt ccagtgccag aatagtattg ttgaaatcag
     8221 gcgtttgcgg gttaagcgcg atggcagcaa tttctgcccg cttttgctgc attccctcat
     8281 cgaatgccgg gcgatagtga tgattggcaa tttgatcaaa atgaggagcc agatacggca
     8341 gtgtgctttg cacaaggaaa ggattcattg ttgtcatttt cttctcctga acgcgaggtg
     8401 ttccatagcg taggcttact gataacggag tgcaatcttg caaaccagta ttacccgctc
     8461 ttaagcatca cgtgctatgt tattgacaca caaaagcgtt gaggaacagt gagatgatcg
     8521 ttttagtaac tggagcaacg gcaggttttg gtgaatgcat tactcgtcgt tttattcaac
     8581 aagggcataa agttatcgcc actggccgtc gccaggagcg gttgcaggag ttaaaagacg
     8641 aactgggaga taatctgtat atcgcccaac tggacgttcg caaccgcgcc gctattgaag
     8701 agatgctggc atcgctccct gccgagtggt gcaatattga tatcctggta aataatgccg
     8761 gcctggcgtt gggcatggag cctgcgcata aagccagcgt tgaagactgg gaaacgatga
     8821 ttgataccaa caacaaaggc ctggtatata tgacgcgcgc cgtcttaccg ggtatggttg
     8881 aacgtaatca tggtcatatt attaacattg gctcaacggc aggtagctgg ccttatgccg
     8941 gtggtaacgt ttacggtgcg acgaaagcgt ttgttcgtca gtttagcctg aatctgcgta
     9001 cggacctgca tggtacggcg gtgcgcgtca ccgacatcga accgggtctg gtgggtggca
     9061 ccgagttttc caatgtccgc tttaaaggcg atgacggtaa agcggaaaaa acctatcaaa
     9121 ataccgttgc attgacgcca gaagatgtca gcgaagccgt ctggtgggtg tcaacgctgc
     9181 ctgctcacgt caatatcaat accctggaaa tgatgccggt tacccaaagc tatgccggac
     9241 tgaatgtcca ccgtcagtaa tttttatacc cggcgtaact gccgggttat tgcttgtcac
     9301 aaaaaagtgg tagactcatg cagttaactc actcacaagc aagaacgaat gaccgtcgaa
     9361 acgcaactta atcccacaca gcctgtcaat cagcagattt atcgtattct tcgtcgcgac
     9421 attgtccatt gcctgattgc accaggcaca ccgttgtcgg aaaaagaagt ttctgttcgt
     9481 ttcaatgtgt cacgccagcc ggttcgtgaa gcctttatta aactggcgga aaacggcctg
     9541 attcaaattc gtccgcagcg tggcagctac gtcaacaaaa tttccatggc ccaggtgcga
     9601 aacggcagtt ttatccgtca ggccattgag tgcgcggtgg cgcgtcgggc ggcgagcatg
     9661 attaccgaaa gccagtgcta ccaactggaa caaaatcttc accagcaacg cattgccatt
     9721 gagcgcaagc aactggatga tttttttgaa cttgatgaca acttccatca actcctgacg
     9781 cagattgccg actgtcaact ggcgtgggat accattgaga acctgaaagc gaccgttgat
     9841 cgcgtgcgct atatgagttt cgaccatgtt tctccaccag aaatgctgtt acgccagcat
     9901 cttgatattt tctctgccct gcaaaaacgt gatggcgatg cggtagaacg tgcaatgacg
     9961 caacatttgc aggaaatcag cgaatccgtg cgccagatcc gccaggaaaa cagcgactgg
    10021 tttagcgaag agtaattcat ttactctcat cccatccggg atgagaggct tttccccccg
    10081 ccttacggct catgcatgca tcaaaaaaga tgtgagcttg atcaaaaaca aaaaatattt
    10141 cactcgacag gagtatttat attgcgcccg ttacgtgggc ttcgactgta aatcagaaag
    10201 gagaaaacac ctatgacgac ctacgatcgt aaccgtaacg caatcaccac tggcagccgt
    10261 gttatggtta gcggcaccgg tcacactggc aagatcctgt cgattgatac tgaaggtctg
    10321 accgctgagc aaatccgccg cggaaaaacc gtagttgttg aagattgtga agagaaactg
    10381 gcacctctgg acctgattcg tctcggcatg aactaagtgt gtgaatgccg ccgatggcgg
    10441 cattgctttt tttacttcac ggaatatttc gccacggtcg ctttcgcgcc atgcgctaat
    10501 aaagacaagt acgcttccgt cacttttgca gtaaacaagc tattgtctgg caaatcatca
    10561 ccaaagattg ccttaatcgc cagcaatgac tggacgcgcg ctttcccttc ggcactactt
    10621 tgtacagcct tctgaataac aggtaacagt gggtcactga tttctatcag atttccctgt
    10681 tcatcaacac caccgacata acgcatccaa cccgcgacgc ccagcgccag cagatcgaac
    10741 ttgctgtcat gcgccagatg ccagcgaaca gaatccaaca tccgctgtgg caatttctgg
    10801 ctaccatcca tcgcaatctg ccaggttcga tgacgtaacg ccgggttgct atagcgtgca
    10861 attaatcggt tagcgtaatc ttgcaaatca acgccctgca ctttcagcgt cggcgcttgt
    10921 tcctgcaaca tcaagccata cgccgcataa cgataatgtt catcttccat gcagtcatta
    10981 atgtgctgat atccagcaag ataacccaaa tacgccagga atgaatgact gccgttgagc
    11041 atgcgcaact tcatctcttc ataaggcagc acatcactaa ccagttcggc tcccgctttt
    11101 tcccattctg gacgtccggc aacaaagtta tcttctatta cccactggcg gaaaggttca
    11161 caggcaacgc ccgcaggatc gcccacaccg gtaagttgtt cgattttcgc cagcgtatcc
    11221 tctgtcactg cgggcacaat acggtccacc attgttgatg ggaaagtcac gttatcttcg
    11281 atccattgtg ccagttttac atcaacagct ttcgcgtatg aagtgacaac gtcacgcata
    11341 acatgaccgt tttctggcat gttgtcacat gacatgacgg taaatgcggg aagtcctgcc
    11401 gctttacggc gggccagcgc ctcaacaatc actcctgttg ctgttttcgg ctggtgggga
    11461 ttttgcacgt cggcagcgac catcgggtga tcgagcatta actgtccgat cgccggagag
    11521 tggaaatacc ctttttcggt gattgtcaga gagacaatcg cgatttgcgg ttcacacatc
    11581 gcagccaaca cggtttctaa gccatccatc tgtacgtgca aggctttttt aacgacgcca
    11641 acgacgcgag ccgtccacgc atcggccgac atttccgcaa cggtataaag attatcttgc
    11701 tgttgtaaat cggcaatttg ctgttcgccg ccgattaagt tgacctcata atatccccag
    11761 tcactgaaat gttctgtagc aagaatatcg gcatacacgc cctgatgcgc acggtgaaat
    11821 gcaccaaagc ctaaatgaat aattcttggg gccaggttat tacgatcata aacagggagt
    11881 gtcgcttttg ctgataacaa attatttccc ataacaattc cttaaatata aatatggcaa
    11941 gctatatgtt ttgttatatg aataaaaatc ccctctccgg taagagaagg gattgaggat
    12001 ttacagactt ctggaaggtt gcgcagctct tacaacacgc ggttgatctt ccgcagcgtc
    12061 ttccagcgca cttaaatcac ggtctttcac ctctggcatt ttcagcgcag agattaaacc
    12121 aattactgaa tatgccatga tcataatggc gatcggatac caggattccg tcatggtgca
    12181 gaaaataccc gccaggatag gaccaaaacc ggaagcgata agaccaccaa tttctttaga
    12241 aatagccatc cgggtaaagc ggtttttaca gccgaacatt tctgccatgg taatgttttc
    12301 cagagcaaat aatcccagca ccgcacagtt atgaatcaca atcagtgcaa ccataatggt
    12361 gctcggggca tagcttttat ctacaatgat agaaagcatt gaccatgcca gcacaatcgc
    12421 ggaggtattc ataataatat acgggatccg gcgaccaatt ttatcggata accaaccaag
    12481 gaacggaatg gtcataaagc cgagaatcga actaatcatc aatgcatctg ttggaattgc
    12541 tttgttaaac aataacgtct gcactaaata gcctgcaagg aaagtctgaa ttaacccgga
    12601 gttacccgcc tgaccaaaac gcagccctgt tgccagccag aaggatttgc tctggaacat
    12661 gctaccagca ggtgcaggtt ttgctgtggg ttggttgctg tcgttaacct tctcaaagac
    12721 cgggctttct ttcagattca tacgcaacca gatagcaaag accatcacga caacgctcgc
    12781 caggaacggt atacgccatc cccacgcaag cagttcctct ttactgagaa tgaagaacat
    12841 aaaggcccag attgccgttg cgctcaaggt tccgcagtta gttcccatag ctacaaatga
    12901 ggagataatt ccgcgcttac cttttggcgc atattccgcc agcatcgtac cggcaccgga
    12961 aatttccgca cctgcaccca acccctgaat aatacgcagc gtcaccagca agatgggggc
    13021 aaaaacacca atctgtgcat aggtcggtaa cacaccaatt aaggtggtac agatccccat
    13081 catggtgatg gtaataaaga gcactttttt acgccctatt ctgtcgccca ttttgccgaa
    13141 aataaatgct ccgacaatac gcgccacata acctgcaccg taggttccca ttgccaaaat
    13201 taacgccatt gccgttgatg attcaggaaa aaatatttca tgaaacacta acgctgcgcc
    13261 gagcgaatat aactggaaat ccataattca tagatgtttt ttactattct gtgggttttt
    13321 gggtgttttc taagtttttt cagatggttg tattttttct aaaaatccct aatctcgatt
    13381 ttgctgttta tttgaggcct ttttatgtcc catatatgcc ccacagatac cccgcagcca
    13441 aaatcaacaa aatgccaaaa ggttctgttc cagccctgca acaagaaatg ctgcgacgtg
    13501 tcagtaaacg ttatgacgat gtagaagtga tcatcaaatc caccagcaac gatggccttt
    13561 cagttactcg caccgccgat aaagattctg caaaaacttt tgttcaggag acgctgaaag
    13621 atacccggga gtctgctgac gagtggtttg ttcgctaatt aacgagtaaa atcagtaacg
    13681 gctggaaatc attcaatact cacactatct aaaagttcac cagccaacag cggtaagttc
    13741 tttcatacga cgtgccgcgg atcctattat tctagtatga aatccctaac cggttttcaa
    13801 attctccaga ctgctcagat tctcttttat cccacgctgg cgctgataaa tctcgtcatt
    13861 gcaatcgacc tgcgctatcg ctgccgcagt tcttccagtt tctgcatcga taactccatc
    13921 ttctgagcaa taaacctcac ctatgcaata ttttatgtca attatttcaa ttgcattaat
    13981 atcttgaatg gattacatag agttaatgta tccagtactc cctattctct tgcatataat
    14041 acatgttgca acttacatct cagcgctatg aaaaaacacc acctctctct ttatgaaatt
    14101 ctggatttac caaacgctaa tttatcattt caatctacct ttaagtattg catttatctc
    14161 cctacgaggt catactttag gaagttaaat atgaatgata atatacctac agcgcgaaat
    14221 cacaaacaat cgacttgtat tacagaaaaa aacatgccta tatttttaaa cttcacagca
    14281 ggcagtatct tacctgagaa tgagctagca tctttacgtt atattgtgca gcaaaatcaa
    14341 aatgatactg taatcataaa agaacgttat aaaatggata tccgttatat cgaatcagtc
    14401 aatggtttta cagtaaatcc tgtatgcagt aatcatttct ccatatttat ggcgagacaa
    14461 aacactattg ctcgcaacct ggaacagcag atcaacaacg gacgaagttt tgcacaaata
    14521 tctcaggatt ttatgcttca attatcttca aatataggat ggaaaaaagg ggccgaaaac
    14581 gcccttaaaa ataaaatcca ttctcattca tttgttgtaa atcctgatga attctcttgc
    14641 gacacacaat ttcttaagtg cccaataaca ttatgcgttc cagaaaaagg ggtttttgtc
    14701 aagaacgcac tgaactccaa catatgcact ctttatgata agtctgcgtt catgaatctc
    14761 acaagagaac atctacccca ccctctcagc agggaaaaga tagtaaaaga aatgattatt
    14821 gaaaggaata tgtgttattt tgacaccata agtcagcatt tcataattat ggatgcagac
    14881 caacagaaac a
//
LOCUS       DAZGTR010000108        14455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGTR010000108 DAZGTR010000000
VERSION     DAZGTR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.110"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            114..470
                     /locus_tag="ACHLCY_003685"
     CDS             114..470
                     /locus_tag="ACHLCY_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0370860.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(521..1417)
                     /gene="yhaJ"
                     /locus_tag="ACHLCY_003686"
     CDS             complement(521..1417)
                     /gene="yhaJ"
                     /locus_tag="ACHLCY_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP0370861.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1522..2223
                     /locus_tag="ACHLCY_003687"
     CDS             1522..2223
                     /locus_tag="ACHLCY_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP0370862.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2246..2410
                     /gene="yhaL"
                     /locus_tag="ACHLCY_003688"
     CDS             2246..2410
                     /gene="yhaL"
                     /locus_tag="ACHLCY_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP0370863.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2622..3932)
                     /gene="cyuA"
                     /locus_tag="ACHLCY_003689"
     CDS             complement(2622..3932)
                     /gene="cyuA"
                     /locus_tag="ACHLCY_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP0370864.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3960..5291)
                     /locus_tag="ACHLCY_003690"
     CDS             complement(3960..5291)
                     /locus_tag="ACHLCY_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0370865.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5566..6930)
                     /gene="tdcG"
                     /locus_tag="ACHLCY_003691"
     CDS             complement(5566..6930)
                     /gene="tdcG"
                     /locus_tag="ACHLCY_003691"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0370866.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(7002..7391)
                     /locus_tag="ACHLCY_003692"
     CDS             complement(7002..7391)
                     /locus_tag="ACHLCY_003692"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP0370867.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7405..9699)
                     /gene="tdcE"
                     /locus_tag="ACHLCY_003693"
     CDS             complement(7405..9699)
                     /gene="tdcE"
                     /locus_tag="ACHLCY_003693"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP0370868.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9733..10941)
                     /gene="tdcD"
                     /locus_tag="ACHLCY_003694"
     CDS             complement(9733..10941)
                     /gene="tdcD"
                     /locus_tag="ACHLCY_003694"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP0370869.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10967..12298)
                     /gene="tdcC"
                     /locus_tag="ACHLCY_003695"
     CDS             complement(10967..12298)
                     /gene="tdcC"
                     /locus_tag="ACHLCY_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP0370870.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKALM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12320..13309)
                     /gene="tdcB"
                     /locus_tag="ACHLCY_003696"
     CDS             complement(12320..13309)
                     /gene="tdcB"
                     /locus_tag="ACHLCY_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP0370871.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13408..14346)
                     /gene="tdcA"
                     /locus_tag="ACHLCY_003697"
     CDS             complement(13408..14346)
                     /gene="tdcA"
                     /locus_tag="ACHLCY_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP0370872.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTAVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3748 a         3876 c         3377 g         3454 t
ORIGIN      
        1 gtatttatct taaatatcaa aacctttata acatccgaaa aagaaagata cgaatagcat
       61 ttacacaata cccggaatag actttcgtca cttttaatta aagggatgtt tttatgcagt
      121 ggtatttagc cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt cgtaaagagt
      181 actggatgtt tactctgatt aacgccatcg tcggcgctat tatcaatgtc attcaattga
      241 ttttaggttt agagtttcca ttcctttctc tcatttacct ggcagcaaca atcattccgg
      301 tcattgctct gtgtgttcgt cgtctgcacg atactgaccg ttcaggtgcg tgggcgcttc
      361 tctatctggt accgatcatt ggctggttag ttctgtttgt atttgcctgt ttagaaggca
      421 attctggcag caatcgttac ggaaacgatc ctaagtttgg ttcaaattaa tcttagaatt
      481 ggggcgacat ttcgcccctt tttattaata ataacaaata ttattttccg ctaaaaagtt
      541 tgggaatttc ccgcagacac caggatttcg cttcccccat actgtcacga cgccaggcca
      601 taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcaa
      661 tatctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaatgcct
      721 gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc agctgtacgg
      781 tcaacaccgg gcgctcacga gcggtatccg ctaccgcaat tccacgatat ttcacgcgcg
      841 tcacttcaga taacggttcc ggctcctgat gaatcgggtg atcaggcgcg gcaacgtaga
      901 cgttcattaa cgtatagagc ttgcgcgagt tgatctccga cgaggaacga aaatgcatat
      961 ccggcgcgat aacaatatcc gcccgcccct gctccagtcg ttcccacgcc cccgccagca
     1021 cttcggtgat gattgccagt tgggtattgg cttttgccgc cagtttgtcg attaacggga
     1081 aaaaggcagg tgtcggtacc agcgcttcgg tcacaatggt gagatgcgtt tcccaaccgc
     1141 gcgcgagggc ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgccctc
     1201 gctccagcaa catcctcccg acattggtga atttggtgcg atgacccgag cggtcaaaca
     1261 gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc
     1321 gtcccagctc atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc
     1381 gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg
     1441 aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga
     1501 agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca
     1561 gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa
     1621 ttgttaggct atgcctccct gcgtgtgctt aaccaggaag tgctggcccc aggtgccgcc
     1681 tttcagccgc gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagca
     1741 gagtatcgcg acagcgaagg caatcatgtt caggccagcg ccggtgaggc gttgctgctc
     1801 tctacccagc cgggtgtcag ctacagcgaa cacaatctca gcaaagacaa accgttaaca
     1861 cgaatgcagc tttggctgga cgcctgcccg cagcgagaga atccgctgat tcaaaaactg
     1921 gcgcttaata tgggcaagca gcaattaatc gcctcgccag agggtacgat gggaagcctg
     1981 caattacgcc agcaagtgtg gctgcaccat atcgtgctcg acaaaggcga aagtgcgaat
     2041 ttccagttgc atgggccacg cgcgtatttg caatcgattc acgggaaatt tcatgcgctt
     2101 acgcatcatg aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat tcgtgacgag
     2161 gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc
     2221 tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gccagccagt
     2281 gaagcccgtg gtggcgaaag aacctgctcg caccgccaaa aattttggct atgaagagat
     2341 gttgagcgag ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc
     2401 taccgcgtaa agacaggttg ccggatgcgg cgaaaacgcc ttatccggcc tacaaaatcc
     2461 aacaacatta acgaattaaa caacccgtag gccggataag atgcgccagc atcgcatccg
     2521 gcatcaggta caaaacacaa caacatcaac aagttgagca ccccgtaggc cggataaggt
     2581 gcgccagcat cgcatccggc acgataccca aaacctggcg tttatctggc cttgctcgcc
     2641 ataatctcga taatctgccg atccgtttgc tgcatcgaat ggcttgctaa cgcacacagg
     2701 ttggcaatcg actgctcaac atcatgcgcc acgatccctt cattgccggt cacggcggta
     2761 tcatccagcg ccattaacac cgctttccac gcagccgaag cactggtcga aaccttcatc
     2821 gcgcagctgt tcgacgcacc atcgcaaatc atgccgctga catcgccgat catactgctg
     2881 atcgccatcg agatggtttc ataacgccca tccaccagcc atgccatccc ggcggcggcc
     2941 cccattgctg cggtcgttgc ggcacacagc gcagacaaac gcggtaactg gttatggatg
     3001 taaattgcgc tcaaatgaga aagcatcagc gcacgcgcca gccgttcatc atccgctccg
     3061 aagtgttctg ctaccaccac cacaggcatt gttgcggtga tcccctggtt acccgagccg
     3121 gagttactca tagccggaag cgtagcgccg cccatacgcg catcggatgc cgcgctggta
     3181 cgaatcacaa tggatgaaga gagatctttc gccagcaaac cgcgctcgca ctgtttttcc
     3241 agcgtcgcgc caatatgcag cccccactta ccgctcaaac cttcctgcga taacgcacaa
     3301 tttagcttcg cggaatcgag aataaagcgg atcgccgcaa acgggacttc attgacgaac
     3361 ttcaggatct cagccagcgt cgttctggaa agcaccgtca gcggagactc ttgctcgccc
     3421 tctgccacac acgcctgctg ggtaaacacc acaccattgt gcgtctcaat atgcacaatg
     3481 ttggtatgcc cgccgacgat ggtgacacac gcccacttct caccgttcca gactttggcg
     3541 cgtgagaaga ggatttcatt gcaaggttcc tggatcttaa cggagacttt ccccgccgcc
     3601 agcagtgctt tggcatcggc aattgcctgc gcagttgcgt ctttcagcac ttccagcccg
     3661 gcgttggcat ttccacctaa cgcccccagc gccgccgcaa tcggcagccc caccattccc
     3721 gtgccgggaa cggtgacgcc cagaccgttc ttcatcagat ttggcgaaac ccaggcttct
     3781 acacgttcaa ccggaccttc cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa
     3841 atcggttcag tacatcccag cgccggtttt acttcctcct gaacggcgag gatgtaacgc
     3901 tgccataacg gatttaaagt cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat
     3961 caagaaaatg ccaggaacgg agaaacacaa agcaacaaac cagtgacgat aatcaggtac
     4021 agagacatcc ctttgtattt gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc
     4081 aggcacccta ccatgccgaa aatcgggcta cagatagagg tgaagctcaa caccggtgcg
     4141 ttcagtacga tggcgctcca ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca
     4201 agattttcgt taatcttctc ggcaggcatc ttgcgacgca ggatgttcat tacgatccct
     4261 tgcgttgctt cgcgaaagcc taaatagacg ccaaagaatg cagtcatgac agcaaagata
     4321 ttgagaatga cgctgaccac tttcacccac gctgcgccgt caccgctaat aaactgtgcg
     4381 gcaatcgcca gcgcggaaat attctgctca taggctttta ccgcttcgtc atgtcccatc
     4441 gccagcgtga acgacacggc gtagaaaaag acggtgacaa acaaaatgcc aaacgcgata
     4501 ttcattgccc gcaatgcttt atgccgcgcc acttcaattg atttttcccg cgagcgataa
     4561 gagatcacca tcgggcttaa cgtctggata aacagaatcg acgtcagggt aaacggcagc
     4621 gtaataatgg cgtttttcac cagcagcccc agcggcggta gcgaaccgac gttatacaga
     4681 tgccacattc ccaccatcga cacgcccagc gccgcgacca ccagcagctt ggtcagcacc
     4741 atgccggtcg aaattttgaa taacaatttc tcgccgcgtg aagagatcgc caccagaatg
     4801 caaatcagta ccagaccata aaagggactg tctgacagca acccttccgt cacgccgaag
     4861 gtatgcagat aggaagcact atcgttggtg atggcggtgg aatagacgaa catccaaatc
     4921 accagcatta cgaaatagag cgcacctaac aggatgcccc agtttttacc taaataaccg
     4981 ctaatgacac tcgggtaatc tttacattct ggtgattctg ccagcgtatt aataaacaac
     5041 cgctgaaaca gatacattgc cgggtaacca atcaccgatg agagcaaaaa tacccacaat
     5101 cccatcaaac cgacctgcac cgggagaaaa acaatccccg cgccaatcgc catcccgata
     5161 ctcataatca cccagccggt gtcagtactg tcgaatttga tcgcttctcg ccactcgctc
     5221 tcactcattc cagcacgacc cgccggggtc gaagcgtctg caatgacgcc tttattcgat
     5281 gcaatttcca taatttctcg ctcaatattt tgtagggctt attatttttt tccgagccgc
     5341 atcaaggcga tatgcggtct acgtttttgc aggcgagtta ttagaataaa gaaatgatac
     5401 gcgcgaggtc agagaaaatc ttcacaatca agactttgaa ataacggaga tggataagaa
     5461 ttttctacta aattaatcgc aagagaatat tcattctcta tttgcggcag atcacaaaaa
     5521 aaaggtgcac atttgtgcac ccaaggatga aagctgacag caatgtcagc cgcagaccac
     5581 tttaatggcc agtcctccgc gtgatgtttc gcggtattta tcgttcatat ctttgccggt
     5641 ttcatacatc gtctcgatca ctttatcgag tgaaacacgc ggtgccgagg tgcggcgcat
     5701 cgccatccgc gcggcgttta ctgctttcac ggcattaatg gcattacgtt caatgcacgg
     5761 gatttgtacc tgtccggcaa ccggatcgca ggtcagccca aggttatgct ccatcgcgat
     5821 ttccgccgca ttgcatacct gcgccggact gccgcccagt agttcagtta accctgccgc
     5881 cgccattgaa caggccacgc caatctcccc ctgacagccg acctccgcgc cagagatgga
     5941 ggcgttcatt ttatacagcg cgccaatagc ccccgctgcc agaaaatagc gggcaattga
     6001 ccgctcgttt accggacgac ggaacttatc gtaataagcc agcactgccg gaataatgcc
     6061 gcacgcaccg ttagtcggtg ccgttaccac gcgcccgcca gctgcgtttt cttcactaac
     6121 cgccagcgcg tacatgttga tccagtcgat gacattcatc ggatcgttag agatgttatc
     6181 gctggaaacc agctgacgac gcagcgctac ggcacgacgc ggcacattga gcggaccagg
     6241 cagcacgcct tcagtgttca tcccgcgttc aataccgtca tgcatcactt gccagatacg
     6301 ggcaaaccct gcgtcaattt ccgctttgct gcgcagcgcc agctcgttgt gcatcatcag
     6361 gccagatata gacaggccgt tgtaatcaca cattttcagc agttcacctg ctgagtggaa
     6421 atcgtaaggt acggacgttt cgacatcgtg cgacaggccg aagtgttctt cttcgacaat
     6481 aaacccgccg ccgacagagt aataggtttt acttaatagc gcttcctgtc ccttccaggc
     6541 agtgatccgc attccgttct catggcgagg caacatttcg ggatggaaga taatgttctt
     6601 tgctacagga aaatcaacaa tatgcgcacc agatgccact ggcagccgcc cgctgcgcgt
     6661 tactaactct ataaatgcag ggatctcatc aatgacaaca tcctgcggac tgtttcctgc
     6721 cagtcccatg ataatggcga catccgtggc atggcctttg cccgtcagtg acaacgaccc
     6781 atacagatcg accacaatat ggctcgtcgc ggttaataag ccgctacttt ccagccgatc
     6841 aataaaactt tttccggcat tcattggccc cacggtatgc gaactggagg gaccaatccc
     6901 aattttgaaa atatcgaatg cactaatcat atccacaccc tcggattgcc gttcagtgaa
     6961 gtggagcgga acgaccttgc gaccgtcccg ctcacgaggc tttacgcact acgtactgcg
     7021 atagcttcaa tttccagctt cacatctttc ggcaaacgcg caacctgcac acagctccgg
     7081 gtcgggtagg tcgcctgatg ctcatcgaag aactgcttat agacttcatt gatggtggca
     7141 aaatcattca gatcggtgat gaacacggtc atcttgatga tatcgcccac gctcagcccg
     7201 gcagcaacca cgatcgcttt gacgttttcg aggcttaaac gcgcctgatc ttgcacatca
     7261 gccgggatct caccggtctg cgggcaaacc ggaatttgcc cggaggtgaa gaccatgctg
     7321 cctaaatcaa cgccctgaac ataagggccg attgcgcctg gggcacgttg cgtttcgata
     7381 atctttttca tacatcctcc ggcgtcagag cgcctgggta aaagtacgtg aaataacatc
     7441 ctgttgctgt tcacgggtca gtgcgttgaa gcgcacggcg tagccagaga cacggattgt
     7501 caggttagga tatttttccg ggtgctcgat ggcatccagc agcatttccc gattcattac
     7561 gttgacgttg aggtgttgac cgccttcgac atccgcttcg tggtggaaat acccatccag
     7621 caggccgaca aggttggttt tacgtactgg atcttctttg cccagcgccg caggaacgat
     7681 tgagaaggtg tacgagatcc catctttggc gtaggtgaac ggcagtttcg ccaccgacgt
     7741 caatgaggcc acggcacctt tgcggtcacg accatgcatc gggttagcgc ccggcgcgaa
     7801 cggtgttccg gcgcgacgac cgtccggcgt attaccggtt ttctggccgt acaccacgtt
     7861 agaagtgata gtcagaatcg actgggtagg gacggcgttg cgataggttg gcagcgcttt
     7921 aattttcttc ataaagcgtt caaccaggtc gcaggcaatg ctgtctacgc gctcgtcgtt
     7981 gttgccgtac tgcggatatt caccgtcgat ttcaaagtcc accgccaggc cgttttcgtc
     8041 acggattggt ttcacgcggg catatttgat ggcagacagg gagtccgtcg ccaccgacag
     8101 gcccgcgatg ccgcatgcca tagtgcgata gacatcacga tcgtgcagcg ccatcagcga
     8161 agcttcgtag ctgtacttgt cgtgcatgta gtgaatgata ttcagcgcgc tgatgtactg
     8221 caccgccagc cagtccatga agtgatcgag gctgtccatc actttgtcgt aatccagcac
     8281 gtcgtccatc agcggtgctg ttttcggccc gacctgaatc ttcagcttct cgtccacccc
     8341 gccgttaatt gcgtagagca gcgttttcgc caggttagcg cgtgcaccaa agaactgcat
     8401 ttgcttacca atcaccattg ggctgacgca gcaggcaatc gcgtaatcgt cgctgttgaa
     8461 gtcagtacgc atcagatcgt cattttcata ctgcaaggaa gaggtgacga tcgacacctg
     8521 cgcggcatat tttttgaagg caatcggtaa ttcttccgac caaagaatgg tcaggttagg
     8581 ttccggtgcc ggccccatag tgtgcagggt gtgcaaatag cggaaggagt ttttggtcac
     8641 cagcgtacga ccgtccagcc ccatcccgcc gatcacttcc gtcgcccaga ttgggtcgcc
     8701 ggagaacagc gaatcaaatt ccggtgtacg caggaagcgt accatacgga tcttcatgat
     8761 gaagtgatcg atcagttcct gtgcctgctg ctcattgagt acgccagctt taaagtcgcg
     8821 ctcaatgtag atatcgagga acgatgccgt gcggcccagc gacatcgcgc cgccattttg
     8881 cgatttcact gccgccagat aagcgaagta gagccactgc accgcttcct gcgcattctg
     8941 cgccgggcga gagatatcaa agccatattt cgccgccatt tcctgaatct gcaacagcgc
     9001 atgacgatgc tctgccagct cctcacgcag acggatggtg gcttccagat cctcgccttt
     9061 ttccagacga gactggagat cggcaaattg cagttcgcgt tcacgtacca gataactgat
     9121 gccatacagc gctacgcggc gatagtcacc gataatgcgc ccacggccat agccatctgg
     9181 taaaccggtc agcacgccag atttacggca gcgcagcata tccggtgagt aaacatcaaa
     9241 tacgccctgg ttatgggttt tacgcagatc ggtaaacaga tattcaaatt cactgtccat
     9301 ttcacggcca taggcgtgga atgaactttt aatcatatta atgccaccga acgggtgtag
     9361 cgcacgtttc aacggcgcat ccgtttgcag gccaacaatt ttttccagcg gctggttaat
     9421 atatcccgca tcatgagcgg taattgtggt ggcaatattg gtatcgaaat caaccggcgc
     9481 gtgggttgcg ttttcgatac ggataccttc cattactttt tcccacaatt ccgtggtggc
     9541 aggcgtcgct tcggcgagga aagattcatc gccttcatac ggtgtatagt tatgttgaat
     9601 aaaatcgcgg acattaattt cgtttttcca gtccgtacct ttaaagccaa gccatgcgtc
     9661 ggcgtacagc ttatcgctgg tatcaatatc taccttcatg aaaaataatc tctctacaat
     9721 acttcaacta aattatgcaa attctgcggg cgcgttaact ttgcctaaat gaatggcatc
     9781 caaagcaatc attttttctt cgttagtcgg aataacagcg caaatgacac gcgcattttc
     9841 actggaaaca attcgctcac cacaggagtt agagcgatta ttcatttctg tatcaatctc
     9901 tacgcctaat acagccaaat gttccatgac cagacgacga attaagcttg aattctctcc
     9961 tattccgccg gtgaatataa ttccatccag gcgacgtaat gaagctgcgt gtccggcaat
    10021 atgacgggca attcggtgaa caaaggtttt aattgccagt tgcgcgcgtt cgtgaccttc
    10081 atgccaggct ttttccagaa cacgtaaatc cgaagaaaga ccggaaatac ctaataatcc
    10141 cgactcttta ttcactacgc gttccaggtc acccaggctc tggttggttt ggctggcgac
    10201 ccaggacatc gcaccaaagt cgacatcgcc actgcgggta cccatcatca agccttccag
    10261 cggcgtcatt cccattgaag tatcaacact ctgaccgttg cgaaccgcgc agattgacgc
    10321 gccattgcca agatgcgcca caaccaggcc ggagtcatct tccgccagat tcagcagcga
    10381 atgtgcgcgc tgggaaacat agcggtgcga cgtgccgtgg aaaccataac ggcgtacacc
    10441 taactcttca taatatttcc acggcaggcc gtataaataa gcttccggag ccatcgtctg
    10501 gtggaaactg gtatcaaata ccgccacctg agttacgccc ggaaataatt gctgcgccga
    10561 ttcaatacca cttaaattgg cgtaattatg caggggtgcc agtggagaaa cgcgacggat
    10621 attatcaatg acttcatcgg taataatggc ggactcggta aaaatactgc cgccgtgagc
    10681 gatgcggtgg ccaattaagg ccacactgtc atttaaattc cgtttttcca gttcaaatgc
    10741 aattgccttc aatgcacctt cgtagctgtg gtgagccagc ggtgctggct ctcccccatt
    10801 tacggataag aatgcatttt ccgagttaat accgtcggca atacctgaca ttaatacttc
    10861 acagtcgctg gcatcgagta cggaaaactt aatcgaagac gaaccacagt taataaccaa
    10921 aacaaccgga aattcattca tctcttttct catcctgagt tacggattaa aacagtttgt
    10981 atacgatgtt caggatggtc agcagaccaa tcacggtaac aaacacgtta tccagacgac
    11041 cacggtattt cgccagagac ggcgctttac ggatggcata catcggcaac aggcacagca
    11101 gggatgcgat aatcggtgcg cccatggctt caataaggtc gaggatgttc gggttggcgt
    11161 aggcaacaac ccaggtggag cccatgatga agatcatgct gatagtgttc agtttaccca
    11221 gcgacacttt agttttgtcg cctttataac cgaacttcag aatcagacca ttcaagcctt
    11281 ccagcgtccc cagatagtga ccgaagaaag atttgaagat agccacgagt gcgatgatgg
    11341 aagccgcata ttccagtgta atcgcgaacg ttgttttggt accggtcatg gacgcaaagt
    11401 ggttagccag ataagaaagc actggaatat tctgcgcttt ggcttccgcc atgttggccg
    11461 gagacagcgt aaacaggcag ctaaaggcaa agaacatcac cactgcaacc atcagcatgc
    11521 tggcacgaga aatgatttgg gaacatttac gttcggtgaa gtcgcgaccg aagtctttct
    11581 catactcttc acgtttagaa accacgaagg aagagacgat tggcgagaag ttaaaggaga
    11641 aaaccatgat ggaaatcccc agccagacag tgatcaggat accgtcatga ccggttaacg
    11701 acagcgaacc gaggtcaacc tggtcgataa ctgcagagtt ccagtaaggg atcagcgaca
    11761 gagaaatcag caccaggctg gcgataaacg gccataccag gtagctcatc actttaacca
    11821 tcagagcctt accaaaccag atgacgaaag ccatcagcag caacaggaac agcgccacaa
    11881 agccgcgatt cagcggtgca aatccgagct ggttttccca gaacgtcata aaggtattgg
    11941 taatagtaac gccataaatc cacagcagtg ggcaaatcgc gaagaagtac aggaacgtga
    12001 taaccacgcc gccagtttta ccaaaatgct cttccaccgt ttccgtaatg ttgccggaag
    12061 ggttagagcc agaaagacac agacgcgcca gcgcccggtg gcaataaaat gcgatggggt
    12121 atgccaatac caacatcaga agaatcggga tcagtccgcc aaaacctgcg cggataggga
    12181 agaacagcac cccggcgccg attgccgtac caaacaagcc taacgtccat gtggtatctg
    12241 atttacgcca ggacgattgt tttgtctggc tggatacaat gctatctgaa gtactcatat
    12301 cctatcctca acgaattaat taagcgtcaa cgaaaccggt gatttgagag acgcgagaaa
    12361 gatcgatatt gccgccggaa ataatactga cggtttttct gttttgaata tattggtcta
    12421 atttaccgct taataatgca gcacatgcca gagcgcctgc gccttcggtg acgactttat
    12481 tgcgctgaat taaggcaatc atactgtttc tgatttcgtc ttcgctgacc agcacgatgt
    12541 catcgactaa ttcacgaacg atttcgtaag ttaaattacc cgggcgggag acatcacaac
    12601 catccgccag ggtgccggta gttcggtgcg tggttatttc tccggagtgg aaagaagccg
    12661 ccatgccgtg aacgttttca gactgtacgc caataacacg aatggtcggg ttaatagatt
    12721 taattgccac cgcaatacca gcaattaaac cgccaccacc aattggcaca atcacgttat
    12781 cgacatcata gagatcttcc ataatttcca gaccaatcgt tccctggcca gcaatcactt
    12841 tcggatcatc gtaaggtggg ataaaaatac ggccttccat ttcgacaatt tcgctcactt
    12901 tagcgatagt gtcgttgaaa ttatcaccat gcagaacgac ttctgcggag tagtcgcacg
    12961 ttgccgctac tttggatttt ggcgcacctt ttggcatcac cactttaccg tcgataccca
    13021 gcatcgcgca ggagagcgaa accccttgcg catggttgcc cgcagaacag gccactacac
    13081 ctttacgttt ttccgcatca gtcagtgaac ttaatttatt aaatgcgcca cgaattttaa
    13141 atgaacccgt acgctgcata ttttcaaact tcaggaatat ttcacctttg caacgttcac
    13201 taaaatagtt tgagcgaggc atgcctgttt tataaattcg cccagccagt cgttgtttcg
    13261 cttcaataat gtcatcaata gcaaccggca gatcgtatgt aatatgcatt ataaaacctc
    13321 ttcgcctgaa ttaaatatgt aggtaaccgt aaccgacacc tgcaaaacag acaggtggat
    13381 tatttatgtt taaataaaaa caaataacta accaacttct attaattgcc ttcgtctaca
    13441 cccattataa gatgaatact ctttggctaa ttccaccaaa accgatgctg cttttttaat
    13501 acgataattt ttcgaccata ccgcggcata ttgtgccaca ggtaatgttt cttcaaccgg
    13561 aatagtaata aattgatttg aaccaaaagg tgacgtcata tcacaaggaa ttacagttaa
    13621 gaaatcagca ttgagaacaa gattataaat tgtcacgact gagtcggttt taacgatgtt
    13681 ttcaatactg atgccatttc tttgtaacgt agtaagaagc tcgctgtagt accccatatt
    13741 ggtttgtggc aacacccact gttcgttctt caacgactcc agcgtggtgg tgccggtgca
    13801 tgttcgggac ttactggcta ccagcacaaa ctcggactcg aacagcggct caacatgtaa
    13861 atcctgaagc ttcatttctg cacttaacgt accaatcgca aaatccagtc gaccgtcgcg
    13921 gattgccggt aagaacgaag acagttgcgc ttcatacata gaaacctgcg ctttcgggaa
    13981 cacctctttg aacttgttga tcatccctga cataaaagta aaaccaatca atgaaggaaa
    14041 accaaatgag acttccacca ccgcctcaga agacataccg cttatctcat taaccatatt
    14101 tttcatttca cgggtaatgg attcggaacg ggagagtaac aattgaccgg caggtgttaa
    14161 tgttacagcg gtatttttcc gcaccactaa ttccacacca aaataatctt caatatcgtt
    14221 aatgatttta ctgacggccg gttgagttaa ccctaattct tttgcagccg agccgataga
    14281 gccacttcta atgacttcct gaaagactac caggtgctgc gttttcggaa gaagaatagt
    14341 gctcataacg acctacgtta attacctcat tgacggcatg aagtgtatca aaatgaaatg
    14401 aacaggatat gtgcgaccac tcacaaatta actttcaata ctttccatgg tatcg
//
LOCUS       DAZGTR010000109        14222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGTR010000109 DAZGTR010000000
VERSION     DAZGTR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.111"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            182..1240
                     /gene="citC"
                     /locus_tag="ACHLCY_003698"
     CDS             182..1240
                     /gene="citC"
                     /locus_tag="ACHLCY_003698"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP0370873.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1255..1551
                     /gene="citD"
                     /locus_tag="ACHLCY_003699"
     CDS             1255..1551
                     /gene="citD"
                     /locus_tag="ACHLCY_003699"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP0370874.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1548..2456
                     /gene="citE"
                     /locus_tag="ACHLCY_003700"
     CDS             1548..2456
                     /gene="citE"
                     /locus_tag="ACHLCY_003700"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP0370875.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2467..3999
                     /gene="citF"
                     /locus_tag="ACHLCY_003701"
     CDS             2467..3999
                     /gene="citF"
                     /locus_tag="ACHLCY_003701"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP0370876.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLSYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTQVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4003..4554
                     /gene="citX"
                     /locus_tag="ACHLCY_003702"
     CDS             4003..4554
                     /gene="citX"
                     /locus_tag="ACHLCY_003702"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP0370877.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4529..5407
                     /gene="citG"
                     /locus_tag="ACHLCY_003703"
     CDS             4529..5407
                     /gene="citG"
                     /locus_tag="ACHLCY_003703"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP0370878.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5458..6921
                     /gene="citT"
                     /locus_tag="ACHLCY_003704"
     CDS             5458..6921
                     /gene="citT"
                     /locus_tag="ACHLCY_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP0370879.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPRAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7035..7841
                     /gene="rna"
                     /locus_tag="ACHLCY_003705"
     CDS             7035..7841
                     /gene="rna"
                     /locus_tag="ACHLCY_003705"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP0370880.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8071..8481
                     /gene="rnk"
                     /locus_tag="ACHLCY_003706"
     CDS             8071..8481
                     /gene="rnk"
                     /locus_tag="ACHLCY_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP0370881.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8619..8708
                     /gene="yldA"
                     /locus_tag="ACHLCY_003707"
     CDS             8619..8708
                     /gene="yldA"
                     /locus_tag="ACHLCY_003707"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP0370882.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8712..9950)
                     /gene="ybdR"
                     /locus_tag="ACHLCY_003708"
     CDS             complement(8712..9950)
                     /gene="ybdR"
                     /locus_tag="ACHLCY_003708"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP0370883.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10171..10599
                     /gene="uspG"
                     /locus_tag="ACHLCY_003709"
     CDS             10171..10599
                     /gene="uspG"
                     /locus_tag="ACHLCY_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP0370884.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10720..12285)
                     /gene="ahpF"
                     /locus_tag="ACHLCY_003710"
     CDS             complement(10720..12285)
                     /gene="ahpF"
                     /locus_tag="ACHLCY_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP0370885.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12456..13019)
                     /gene="ahpC"
                     /locus_tag="ACHLCY_003711"
     CDS             complement(12456..13019)
                     /gene="ahpC"
                     /locus_tag="ACHLCY_003711"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP0370886.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13391..14137
                     /gene="dsbG"
                     /locus_tag="ACHLCY_003712"
     CDS             13391..14137
                     /gene="dsbG"
                     /locus_tag="ACHLCY_003712"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP0370887.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3380 a         3759 c         3582 g         3501 t
ORIGIN      
        1 tgtgaaataa atcaaaattt cacgccgaaa tactccttag gatgtatagc gaaaggagaa
       61 aaagatatac ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc
      121 aacattgaac aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat
      181 tatgttcggc aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga
      241 aatcgcccaa ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat
      301 taccgtaacc cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa
      361 atgcgttgct atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga
      421 actgataaac ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga
      481 atacgaggcg ctgttccgcc agtgcggttt ttccacgctg accagcgtac ccggcgtgat
      541 ggtgctgatg gaaaacagcg ccacgcggct gaaacgctat gccgaatcgc tgaaaaaatt
      601 tcgtcatcca gggaacaaga ttggctgcat tgtgatgaac gccaatccct tcacgaatgg
      661 tcaccgttat ctgattcaac aagctgcggc acagtgcgac tggttgcatc tgtttttagt
      721 caaagaagat tcttcacgct tcccctatga agaccggttg gatctggtgt taaaaggcac
      781 cgccgatatt ccacgcctga ctgtgcatcg cggctccgaa tacatcatct cccgcgctac
      841 gttcccttgc tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga
      901 tctgaagatt ttccgtcagt acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg
      961 tactgaaccc ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga
     1021 aacgccgact atctccgcac cgcccatcga actggttgaa attgagcggc tgcgttacca
     1081 ggagatgccg atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc
     1141 tatcgcgccg ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc
     1201 ccgccaggac gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa
     1261 ataaaccagc ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc
     1321 ccactcgata cgcaggatat cgacctgcaa atcaatagca gcgttgagaa acagtttggc
     1381 gatgcaattc gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag
     1441 ctgaatgtcg atgacaaagg cgcactggac tgcattttac gtgcacgact ggaagccctg
     1501 ctggcacgcg ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt
     1561 cgctgcaaca acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg
     1621 ccgcgatggt cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag
     1681 actccgtggc attacgtgaa aaagacaccg cccgccgcat ggtttatcac gcgctgcaac
     1741 atccgctgta tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg
     1801 gtgttaacga cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa
     1861 ccgataccgc tcaggatgtt ctggatattg aaaaagagat cctgcgtatc gaaaaagcct
     1921 gtggtcgtga acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta
     1981 cccgcgcagt ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag
     2041 aagactatgt gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg
     2101 cacgctgttc cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct
     2161 attccgacgc taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg
     2221 gctttgacgg caaatcgctg atcaacccgc gtcagattga tctgttgcac aacctctacg
     2281 caccaaccca gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg
     2341 ctcgcgaagg cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg
     2401 atcgcgcccg tctggtgctc tctcgtgcag aactttccgg catccgcgaa gaataaggca
     2461 atcaaaatga cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat
     2521 cgtcgcgctg aatgcgatct tgccgctttc cagaactcgc caaagcaaac ctaccaggct
     2581 gaaaaagcgc gcgatcgcaa actgtgcgcc aacctggaag aagcgattcg tcgctctggt
     2641 ttacaggacg gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc
     2701 aatatggtga tggacgtcat cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc
     2761 tccctgagtg attgccatgc gccgttggta gaacacattc gccagggcgt ggttacccgc
     2821 atttatacct ccggcctgcg cggtccactg gcggaagaga tctcccgtgg tctgctggca
     2881 gaaccggtgc aaatccactc tcacggcggt cgcgtgcatc tggtacagag cggcgaactg
     2941 aatatcgacg tggctttcct cggcgtcccg tcctgcgatg aattcggtaa tgccaacggc
     3001 tacaccggta aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca
     3061 aagcaggtcg tgatgcttac cgaagaactg ctgtcttatc cgcataatcc ggcaagcatt
     3121 gagcaagatc aggttgattt gatcgtcaaa gttgaccgcg ttggcgatgc tgcaaaaatc
     3181 ggcgctggcg cgacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct
     3241 gcggatgtga ttgtcaactc aggctacttc aaagaaggtt tctccatgca aaccggcacc
     3301 ggcggcgcat cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt
     3361 cgcgccgact tcgcccttgg cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt
     3421 ctgatccgca aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc
     3481 cgtaacccca atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc
     3541 gcatcggttg atcgtctcga cgtggtggta ctgagcgcgc tggaaattga cacccagttc
     3601 aacgttaacg tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc
     3661 gataccgcga ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt
     3721 ccgactcagg tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg
     3781 gtcacagacc acggtatcgc agttaacccg gcacgtccgg aactggcaga acgtctgcag
     3841 gaagcgggca ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc
     3901 ggtgaaccac agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat
     3961 ggctcggtga tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa
     4021 ctcgccagcc accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa
     4081 gcacggcaac acgtctggct caagcgccat cctgttccac tggtctcctt taccgtggtt
     4141 gcgcctgggc cgattaaaga cagcgaggtc acacgccgaa tttttaatca tggcgtgaca
     4201 gccttgcgtg ccttagccgc aaaacagggc tggcaaattc aggagcaggc tgcactggtt
     4261 tccgccagcg ggccggaggg catgttgagc attgccgccc cggctcgtga cctcaagctc
     4321 gccaccattg agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg
     4381 acgcccgaag gcgaaattct ctcccgccgc gactattcac tgccgcctcg ccgctgcctg
     4441 ttgtgcgagc aaagcgcagc cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta
     4501 ctcaaccgca tggaggcact gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac
     4561 aaagcttgcg acgtcattaa tcgatgagta cgccctgctg ggctggcgcg ccatgctgac
     4621 tgaagtcaat ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa
     4681 agatatggcg ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg
     4741 tttcattgaa tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tccacggatt
     4801 acgcccaatt ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac
     4861 gcataaaggc agcatttttt ctttagggct gctatgtgcg gcaattggcc gtttgcttca
     4921 gctcaaccag ccggtaacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg
     4981 tctgaccgat cgcgaactgc gtaccaataa ttcacaactg acggcaggtc aacggttgta
     5041 ccagcagctt ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat
     5101 caaccacgcc ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc
     5161 attgctcgat accttgctcc tactgatggc gatcaacggc gataccaacg ttgcttcgcg
     5221 cggtggcgag gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg
     5281 gggcattcga acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga
     5341 acgaaatctc agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca
     5401 gatttaatta tttaagcact tgataaattt gaaaatatta attttcggag aacccgtatg
     5461 tctttagcaa aagataatat atggaaacta ttggccccac tggtggtgat gggtgtcatg
     5521 tttcttatcc ctgtccccga cggtatgcca ccacgggcat ggcattactt cgctgtgttt
     5581 gtggcaatga ttgtcggcat gatcctcgag ccaattccgg caacagcgat cagttttatt
     5641 gcggttacta tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct
     5701 gacccagcgt ttaatgcgca aaaacaggcg ctgaaatggg gtctggctgg tttttccagc
     5761 accacggtat ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg
     5821 ttaggtcgtc gcattgccct tttcctggtg aaattcatgg gcaaacgcac gctgacgttg
     5881 ggttatgcga ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg
     5941 cgtaccgggg gtacggtttt tccggtcatt aaaaacctgc cgccgttgtt taaatcattc
     6001 cccaacgatc cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt
     6061 accagtctga gttcgtccat gtttgtcacc ggtgcggcac caaacgtgct gggtctggag
     6121 ttcgtcagca aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg
     6181 ccggttgggg ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg
     6241 gaaatcacac acagtgaaga agtggcaacc tgggcgggtg atgaactgaa aaccatgggt
     6301 gcgctgacac gcagagagtg gacactgatt ggccttgtat tgctcagctt aggtttgtgg
     6361 gtttttggca gtgaagtcat taatgctact gcagttggtc tgctggcagt ttcgctaatg
     6421 ctggccctgc acgttgtgcc ttggaaagat attacccgct ataacagcgc atggaacacg
     6481 ctggtcaacc tggcaactct ggttgtgatg gctaacggcc tgactcgttc tggctttatt
     6541 gactggttcg ccggtaccat gagtacgcac ctggaaggat tctcaccaaa cgcaacggtg
     6601 attgtactgg ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac
     6661 accgcaacca tgctgccggt tattctggcc gtcggtaaag gtattccggg cgtaccaatg
     6721 gaacaactgt gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccgtat
     6781 gcaaccggtc ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg
     6841 cgtcttggcg caatcttcgg ggtgatttac atctctatgt tgctgttggt tggctggccg
     6901 attctcgcca tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca
     6961 ggtaagccca cctggcgggc ttttttatga tttaatagat agtattcagt gtatgtatga
     7021 gttccacacc cattatgaaa gcattctggc gtaacgccgc gttgctcgcg gtttctctgc
     7081 ttcccttctc ttctgccaac gccttagcgt tgcaggcaaa acagtatggc gattttgatc
     7141 gctatgtcct ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc
     7201 gtaacgaacg agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttctga
     7261 ccgtacatgg tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac
     7321 gccgctggat gcgcttcggt tgcgctactc gcccaatccc gaatctacca gaagcgcacg
     7381 ccagccggat gtgttcatcg ccggaaaccg gattatcact ggaaacggcc gctaaactaa
     7441 gtgaagtcat gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac
     7501 acggtgcctg ctttggtttt gatccggacg catacttcgg tacgatggta cgcctgaatc
     7561 aagaaattaa agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga
     7621 gccgccgtga ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag
     7681 ttaagctaac gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag
     7741 ctgacgccat caacgctccg ctttctgcaa actcattctt gccacaacca cacccaggca
     7801 actgtggcaa aacctttgtg attgataaag tgggttatta atcatctgat ttacaaaaca
     7861 cgctaattcg tcttccataa accgtgacga ttaagaatta tacataatgc gctgcattga
     7921 ggaattccct gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat
     7981 gtgagttaaa ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga
     8041 cactcgcagt accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac
     8101 ctggatgccg aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc
     8161 gccgacgcgt taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca
     8221 cacgacgtgg tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg
     8281 cgtgtgcgca cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt
     8341 atggctccgg taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa
     8401 cttccgggcg gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct
     8461 gctggcgact acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga
     8521 tgcactcgca tcctctttac gcaaaatcag caatttttcc tggttctcac tgttcacttt
     8581 tgctgcaaaa ttattccgtt tcgtttgtgg gaataaccat ggcagaagca ttttacatcc
     8641 tgataggatt tctgattatg gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc
     8701 actcctgacg atcatattgt tccccccggc atcgcattca ccagacctga aaccgccttc
     8761 tgcgccgcct ctgcgctttg tgcaccgggt accagaatca ccttacggca ctcctcttca
     8821 cgtttttcga aaatctcata tccccgggcg gcctcttcaa acggcatata gtgggtaaca
     8881 atttcttctg gtttcagtaa tcctttctca attaacggta ataattctcc cagccatgcg
     8941 tgaacgtggg tctgtcccat tttaaacgtc aaccctttat caaaggcgtc gccaaacagg
     9001 aaaccgtgaa taaatccagc gtagacgccc ggtacgctaa caatgccgcc acgcctgacc
     9061 gccgcaatac actggcgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc
     9121 accgtttccg tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg
     9181 ccccggtgac ctgccgtttg ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa
     9241 ttaatcggga tcgcgccgta gcggtcagcg gcgaaacgca agcggtaggg atgatgatca
     9301 acaacaaaaa tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccact
     9361 ggaccggcac catagactgc aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc
     9421 tgccatgccg ttggcagaat atcagaaagg aaaagggctt tatcatctga aagcaaaggc
     9481 ggtactttaa acggccccac attcccttta gggacgcgga catattccgc ctgtccacca
     9541 ggaacgccac catacaggtg actataacca aacaatgccg cgggagctgg tatctgtttt
     9601 ttattgagcg cagcgccttt acccgcattg gtattttcgc aggcggcata ttgctgcatt
     9661 cgacagaaaa aacagtcgcc acaagcaatg acaaacggaa ttaccacccg gtcgcctttt
     9721 tgcaaatttt ttacgtcctt tccggtttca acgacttccc ccataaattc atgaccaaaa
     9781 atatcgccat gtttaacctg gggtattttg cctcgataaa gatggagatc agagccacag
     9841 atcgccgttg ccgtaatacg cagaataata tcatctgcct gttcaatgcc cggatcggga
     9901 acattttcta cctgaacgtg atgtgggccg tgataagtca atgctttcat gcaacctcca
     9961 gagacagttc aaaaaagagt cagtctttaa gggtagctat cacagcggga aaacgggcgg
    10021 tacctcaggt tttgggagaa ttcctggtgt tcagtgagaa tatattaatc acagaaaccg
    10081 ttcgtaagga aaaagaacca gacaaatatc agggataaat attataatca tggcgaatga
    10141 tttgttcatg attaacaggg agaaagggtt atgtataaga caatcattat gccagttgat
    10201 gtatttgaaa tggaattgag cgacaaagct gttcgccacg ctgaattcct cgcccaggat
    10261 gacggagtta ttcatctact tcacgtacta cccgggtcag ccagcctgag cctgcaccgt
    10321 tttgccgctg atgtgcgtcg ttttgaagag catctgcaac atgaagcaga agaacgtctg
    10381 caaacgatgg tcagccactt caccatcgat ccttcccgca ttaaacaaca tgtccgtttt
    10441 ggtagcgtgc gggatgaagt caatgagttg gcagaagaac tgggggctga tgttgtagtt
    10501 attggttctc gcaacccatc gatttcgacc catctgttag gttctaacgc ctcgagcgtg
    10561 atccgccatg ccaatctgcc ggtgctggtt gtgcgttaat tgccagaata aatatcccgc
    10621 cctgcccgga gccatcccgg cagggctttt taatgccata aaaaagccgc caggtttgac
    10681 cccggcggct aagcaattgc aggtgaatct tacttcttct tatgcagttt tggtgcgaat
    10741 caggtagtca aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgataatctg
    10801 cttgtacgga accgtcgtac agtcacccgc tgcgaacacg cctttgacgt tggtttcgca
    10861 tttcgcatca atgataatct cacccatgcg gttacgttcg actgcgcctt ccagccagtt
    10921 ggtgttcggc agcagaccaa tctggacgaa aataccggcc agttcgatgt tgtgaatatc
    10981 gccgctgaca cgatctcgat attccagacc aacgactttg ctgccgtcgc ctttcacttc
    11041 cgtggtttgc gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag
    11101 aacctggtcg gctttcattt ctggcgcaaa ttcgagcagc gttacgtgct caacgatacc
    11161 cgccaggtca attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt
    11221 gcctttaaac agcgggccgt cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg
    11281 atcttcgcca ggaacgttca tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg
    11341 tgctttcagt accgcgccag aagctgtttc aatctgatgc agaccacctt caactgctgc
    11401 tgggatcagt ttgctggcgc tctggctgtc gatcacatca acgtcgtatt catcaacgtg
    11461 aactttcagt gcgcctgcca gcttctgccc ttcagtcttc ggtacagaaa tgtagttttc
    11521 gatatcaacg gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat
    11581 gcctttacgt gcggagtaaa ttgctgcagc tgcacccgcc gggccggaac caacgattaa
    11641 tacgtcataa gcatcacgct tgttcagctc ttctgccgca cgtttttccg cgcccgtatc
    11701 aattttggca acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac
    11761 gaacactgcc ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc
    11821 gtcaattgca gtgtgcttga tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac
    11881 cacgtccggg cagttatggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc
    11941 aatatggcga atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca
    12001 tagcaacgcc agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg
    12061 cccctggttg gaacctgggt tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt
    12121 atcttcttta aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat
    12181 ttctgccgat ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt
    12241 ctcaaggtaa gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc
    12301 tttaaacatc atcatgcaag cggcctctgc ggctacctgg atgcagcttg catcatggtg
    12361 cggggaagaa aatcgggtgc cgcagcaccc gaagaattag gtgaatttcc gaagtatttc
    12421 gcgccggagt tggtagcgcg aaagacgaag gaaatttaga ttttaccaac caggtccaga
    12481 gacggagcca gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga
    12541 gaagctacgt actgtgctgc tttgatttta cgcagcaggt cagacgcgtc acggccaatg
    12601 ccttcagcgg taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca
    12661 cggtcagcca gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc
    12721 gggtcgccga tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct
    12781 ttgtgggtga agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt
    12841 tcttcgtagt ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc
    12901 gggtagaaga agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat
    12961 tcgccgtttt tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc
    13021 tatacttcct ccgtgttttc gatgagatgt aaggtaacca atttttgccg attcggctaa
    13081 tgcgtttcca ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg
    13141 aactttgcac cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta
    13201 atctgaacta ccctttggta tcttcagtgc ctgcctgcag gctgaggtgt tgcccttaca
    13261 aatgcaacaa cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa
    13321 gctcttacct atgacagtga taggttatgc cttttactcg acttttgcac tgactgaaaa
    13381 ggacaaatta atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc
    13441 agaggaactt cctgctccag taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac
    13501 attcgatgcc cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac
    13561 catctacctg actccagatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg
    13621 tgaaaacctg agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat
    13681 gtggcaacgg atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat
    13741 tgtctacgtg ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg
    13801 cccgtgggta gattctggca aagtgcaatt aagaacattg ttggttgggg ttatcaagcc
    13861 agaaagcccg gcgacagcag cggcaattct tgcctccaaa gatcccgcaa aaacctggca
    13921 agaatatgaa tcctctggtg gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga
    13981 acaaatgaaa gtgttaagcg ccaacgagaa actcatggac gatctggggg caaatgtcac
    14041 accagcgatc tattacatga gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga
    14101 tcagaaaacg cttaatatca ttatggggaa taaataaaag ataattcatg gatggttgaa
    14161 atacatccct gcttttcaac catcctaaaa aagcatgaat tgctcgctct gccctaagtg
    14221 gc
//
LOCUS       DAZGTR010000110        14194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGTR010000110 DAZGTR010000000
VERSION     DAZGTR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.112"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..582
                     /locus_tag="ACHLCY_003713"
     CDS             16..582
                     /locus_tag="ACHLCY_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0370888.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            633..3092
                     /gene="ybgQ"
                     /locus_tag="ACHLCY_003714"
     CDS             633..3092
                     /gene="ybgQ"
                     /locus_tag="ACHLCY_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0370889.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3107..3838
                     /gene="ybgP"
                     /locus_tag="ACHLCY_003715"
     CDS             3107..3838
                     /gene="ybgP"
                     /locus_tag="ACHLCY_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0370890.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3835..4896
                     /gene="ybgO"
                     /locus_tag="ACHLCY_003716"
     CDS             3835..4896
                     /gene="ybgO"
                     /locus_tag="ACHLCY_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0370891.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVLGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5048..6094
                     /gene="abrB"
                     /locus_tag="ACHLCY_003717"
     CDS             5048..6094
                     /gene="abrB"
                     /locus_tag="ACHLCY_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0370892.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHLCY_003718"
     CDS             complement(6091..6882)
                     /gene="nei"
                     /locus_tag="ACHLCY_003718"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP0370893.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNELTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERVLSPRFRNRQFAGLLLE
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHLCY_003719"
     CDS             complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="ACHLCY_003719"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP0370894.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHLCY_003720"
     CDS             complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="ACHLCY_003720"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP0370895.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHLCY_003721"
     CDS             complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="ACHLCY_003721"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP0370896.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHLCY_003722"
     CDS             complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="ACHLCY_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP0370897.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHLCY_003723"
     CDS             10261..11742
                     /gene="dtpD"
                     /locus_tag="ACHLCY_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP0370898.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHLCY_003724"
     CDS             complement(11784..13202)
                     /gene="phrB"
                     /locus_tag="ACHLCY_003724"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP0370899.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13199..13708)
                     /gene="ybgA"
                     /locus_tag="ACHLCY_003725"
     CDS             complement(13199..13708)
                     /gene="ybgA"
                     /locus_tag="ACHLCY_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP0370900.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13859..14194)
                     /locus_tag="ACHLCY_003726"
                     /pseudo
     CDS             complement(13859..14194)
                     /locus_tag="ACHLCY_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3410 a         3802 c         3458 g         3524 t
ORIGIN      
        1 ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
       61 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
      121 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
      181 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
      241 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
      301 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
      361 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
      421 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
      481 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
      541 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
      601 tatcgccgcc tttcataaaa tatttatcag gtatggacac agtgaatatt tatcgactct
      661 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta
      721 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      781 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
      841 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
      901 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
      961 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1021 taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
     1081 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
     1141 accgcccaca ggatggcagc agcagcacta atcttaacgc ctatggaaca gccggaatca
     1201 acacgggggc atggcgctta cgcagtgatt atcagttgaa tcagactgat agcgatgata
     1261 accatgaaca gtcaggcgaa atatcacgta cctatctttt tcgcccgtta ccgcaattag
     1321 gttcaaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
     1381 ataccggcgc ggcactggca agtgacgatc gaatgttgcc atgggaacta cgcggctacg
     1441 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1501 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
     1561 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1621 aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
     1681 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1741 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg
     1801 gtgatgacta ccattctgcc gcaataggta tcgggcaaaa tatgctgtgg cttggcgcgc
     1861 tgtcgtttga tgtcacgtgg gccagtagtc actttgatac tcagcaggac gagcggggct
     1921 taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg atttccctcg
     1981 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt gatcacaaat
     2041 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
     2101 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2161 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
     2221 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
     2281 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2341 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2401 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga gcccaggtga
     2461 gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2521 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2581 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2641 gcgtggcaga tattccggtt cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2701 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2761 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
     2821 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
     2881 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
     2941 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
     3001 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
     3061 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
     3121 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
     3181 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
     3241 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
     3301 cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacgca
     3361 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
     3421 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
     3481 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
     3541 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
     3601 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
     3661 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacaccgg
     3721 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
     3781 gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
     3841 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt
     3901 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
     3961 ccttttgctg tactaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt
     4021 aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat
     4081 gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac
     4141 aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt
     4201 gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac
     4261 aaaatttgct cgggtgatcc cgctaatatt cacatgtcac gtacctttct cgctcgcatg
     4321 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
     4381 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac tgcccaaaac
     4441 ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
     4501 tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt
     4561 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
     4621 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
     4681 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
     4741 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
     4801 aaaacctata cggctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
     4861 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
     4921 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
     4981 ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
     5041 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
     5101 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
     5161 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
     5221 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
     5281 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
     5341 gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc
     5401 tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
     5461 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac
     5521 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
     5581 agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg
     5641 catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     5701 gtctggtcag ctcatcacca tcgaactccc ggaatggctg cttgcgatgg cgtatatggc
     5761 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
     5821 gctaccgcaa atcctgctgt cgatttttgc tctgttggct atttgtgcgg gtatggcgtg
     5881 ggggctgacc cggtttatgc atattgattt tatgactgcc tacctcgcca ccagccccgg
     5941 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
     6001 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg ggacctgcca ttgcacggtt
     6061 tatttcaacc tatgcgccga agcggtcggc ttagtgctgg cagccagggc accagtaaaa
     6121 cgggcgagat gacagcgtgg ttttctcaat gatgctgcca caacgttcgc acggttcgcc
     6181 atctcgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
     6241 cccccgcgta gcgtaagaaa atcgaggaat ctccagtaac gcgtgtgcga gtgcatccag
     6301 ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat
     6361 ctccacccgc aaataattgc caagtcctgc cagaaacgcc tgctcgagca gtaatcctgc
     6421 aaactgacgg ttacgaaagc gcggcgacaa tacccgttct ttcaccacct ccggcgtcag
     6481 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
     6541 cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     6601 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     6661 gacgccgtaa agctgattat ggctgtagag cgttaattcg ttggaaaaat gagttaacaa
     6721 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
     6781 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     6841 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     6901 ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa tgtagctcgt
     6961 aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc
     7021 gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc
     7081 gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctggctgact tcgcggcacc
     7141 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
     7201 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat caatgccgga
     7261 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
     7321 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
     7381 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcaactgc
     7441 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aattgcaaca
     7501 ccatttttta ttgcttcacg cacgcaagcc tgcatggttt gcgcatcgcc tgcatgaaat
     7561 ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     7621 tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta attgttcaaa
     7681 ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
     7741 aatcggctga ccgagcggaa tttgcgcaag atggtacata tcagcctcaa tgatgcaggc
     7801 aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg gctgcccgtt
     7861 atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcac gatcggtggt
     7921 gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg agctaagctg
     7981 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     8041 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
     8101 atcacgcttc gctttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag
     8161 cccgccaatc cccactttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
     8221 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
     8281 ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat ccagccgcgc
     8341 ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa cagttaactg
     8401 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagta ggttagcaat
     8461 gcgtaacgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     8521 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     8581 tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     8641 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     8701 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagtaag
     8761 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     8821 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     8881 tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca
     8941 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
     9001 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
     9061 accgtaatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
     9121 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
     9181 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
     9241 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
     9301 cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
     9361 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
     9421 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
     9481 ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
     9541 cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc
     9601 ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg
     9661 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
     9721 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
     9781 tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc
     9841 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
     9901 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
     9961 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10021 ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac atattaagaa
    10081 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    10141 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    10201 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    10261 atgaataaac acgcatcaca accgcgcgct atttactatg tcgttgcgct gcaaatctgg
    10321 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    10381 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    10441 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    10501 ctgggggcgt tgttgatggc gatcggtcat gtggtgctgg gtgccagtga gatccatccg
    10561 tcattcctct atctgtccct ggcgattatc gtttgtggct atggtctgtt taaatccaat
    10621 gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    10681 tcgctgatgt atgcagcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac
    10741 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggtggg tatgatcgcg
    10801 ggtctggtca ttttcttatg tggcaatcgt catttcacac atacccgcgg cgttaataaa
    10861 aaagtactgc gtgcaacaaa ctttctcctg cctaactggg gatggctgct ggttctgctg
    10921 gtggcaacgc ctgcgctgat taccgtactg ttctggaaag agtggtcggt atacgcctta
    10981 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    11041 cagaaacagc gtaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc
    11101 tgggccttcg cacaacaggg cggcagctcg attagccttt atatcgaccg cttcgttaac
    11161 cgcgatatgt ttggttatac cgttccgact gctatgttcc agtcgattaa tgccttcgca
    11221 gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag aaagcgtcgc aggtaatcgt
    11281 accgtgcgca tctggggaaa atttgctctt ggccttggcc tgatgagcgc cggattctgc
    11341 attctgacct taagcgcccg ctggtccgca atgtatggtc actcttctct gccactgatg
    11401 gtattaggcc tggcggtgat gggctttgcg gaactgttta tcgacccggt tgccatgtcg
    11461 caaattacgc gcattgaaat tccgggcgtg accggcgtat taaccggcat ctacatgctg
    11521 ctttctggcg cgatcgcgaa ctatctggcg ggcgtgattg ccgatcagac atcgcaggct
    11581 tcgtttgatg cttccggggc gatcaactac tccatcaatg catatattga agtatttgat
    11641 caaattacct ggggcgcact ggcgtgtgta ggactggtac tgatgatttg gctgtatcag
    11701 gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat aataggccag caccatacct
    11761 gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg cttcttcgta cgctgccagc
    11821 gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg gataatccag catcacacct
    11881 gctttctgcg cccacttcca cggctcatgc accgctttcc ctggtacatc gcgcagctcc
    11941 ggtaaccatc ggcggataaa ctcaccttcg cgatcaaatt tctcgccctg ggttgtcggg
    12001 ttgaaaatac gaaaatacgg cgctgcatca gtacccgtag acgccgccca ctgccagcca
    12061 ccgttattgg ctgccaaatc accatcaatc agctgcgaca tgaaatatcg ctcgccttcg
    12121 cgccagtcga tcaacaaatc tttcaccaga aaactggctg aaatcatccg tagccggtta
    12181 tgcatccagc cagtgctgtt aagctgacgc atggcggcat cgacaatcgg gtatcccgtt
    12241 ttgccttcct gccaggcctg taaatgtgcg ggattgctct gccactgcac acgatccgtc
    12301 caggcaataa acggacaatg tttacacaac gaggggtaat acgtcatcag atgacggtag
    12361 aactcgcgcc agatcagctc actaagccag acactaccgg ccccaccgtc cagcgcctgc
    12421 ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg ataacccgcc cgttgccagg
    12481 ctggcggaca aacgactggt gccttccact gccggaaaat ctcgttgttg ctcatattct
    12541 ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg ccgctttttc ttccaccggg
    12601 aaatgcgcag tatcgaaaga ctgacgagga taattcagcg taatggatgg cgagggctct
    12661 atcgatccgc tactacgaac ttttggcgca gcgacgcact ccggcatccc ttcccgcagc
    12721 cgtttcagcc aggcattctt aaaaggcgta aagactttgt acatctcgtg attaccggtc
    12781 atcaccgcgc caggcggcag gatcacgctg tcatcaaatc cttcacacac cacgttacgc
    12841 agcgctcttt caacttccac atcccgcgcc cgctcattca cttcatactg atagttataa
    12901 aacaggtggg taacgctgtt ttccgcgcac acctgtttaa ctatttcgac actggcgaca
    12961 aagtcatcca cttcacggaa caataaagga ataccttttt ccgcaagcgc tatttgtagc
    13021 ccattcagtt gagcattgat aagttcagcc tgacgcggcg acatgttatg cgtcgcccac
    13081 tggcgtggtg tagcgatata caacgccagc acgcgtgcag acgaattgcg gcaggcggca
    13141 gccagtgcga gattatcgtg cagacgtaaa tcctggcgaa accagaccag atgggtagtc
    13201 ataaaactcc tgaatggcgc aagttaatca cacgcggcca aagttcgaaa taacgctgcc
    13261 ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt gatacgcatc agcggcgcaa
    13321 gaagtggttg ctcgccacgg cgataactgt cgataagcgc cgccaactgc tggcgttctg
    13381 tggaatcaat atgcgggcga aaataaccct gaacgtgcat caaaacgttg gtgtgatcgc
    13441 gcgggttggc gggatgagaa agcaaaacaa ttacgcgttg gtagtactgg ttgtaaaaat
    13501 catccagatt ttgccactgg tgaatatcag ccacgaacgg gccaagtttg cggtactccg
    13561 gctgagaatg cgccagaaag acgagtttat agcggctgtg ataatcgaga agcgccccgc
    13621 gcgttaagcc atgttctttc agtcgatgta actcatgaag ggcgaagata cggattaggg
    13681 tgctgtcatc aaatcgttgc tgattcatta ctgctcctga acggtttaca ggagtaagtg
    13741 tagacgttga gaaatgatat ttgtgaagtt aatcctgttg tagcaggaca aatttacgaa
    13801 agcctgctcc ccgcaaggac tgacgtcagg tagtttctaa ccatagctgc cctttctatt
    13861 caaacgtgaa atcaataagc tcatcaggga catcgcaaac aatatgaaga cgggtttctt
    13921 ctctgccgtg actcttttca ctaattgcgt aactgtcatg cttgggatta tttaattctt
    13981 tcagcggaaa tttttcctcg aaggctttat ttagccgccc ctggtttcct tttacagcga
    14041 ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg
    14101 catcggttgt gatgattttt cctttaatat ccagcatgtt aagaagttct gggatagctg
    14161 tgatctcatt ggatttctta tccgtcttga tctg
//
LOCUS       DAZGTR010000111        13799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGTR010000111 DAZGTR010000000
VERSION     DAZGTR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.113"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            87..998
                     /gene="hlyE"
                     /locus_tag="ACHLCY_003727"
     CDS             87..998
                     /gene="hlyE"
                     /locus_tag="ACHLCY_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFP0370901.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QNFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1205..1651)
                     /gene="ycgN"
                     /locus_tag="ACHLCY_003728"
     CDS             complement(1205..1651)
                     /gene="ycgN"
                     /locus_tag="ACHLCY_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP0370902.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1743..2402)
                     /gene="ycgM"
                     /locus_tag="ACHLCY_003729"
     CDS             complement(1743..2402)
                     /gene="ycgM"
                     /locus_tag="ACHLCY_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0370903.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2474..2767)
                     /locus_tag="ACHLCY_003730"
     CDS             complement(2474..2767)
                     /locus_tag="ACHLCY_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP0370904.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            complement(2779..2937)
                     /locus_tag="ACHLCY_003731"
     CDS             complement(2779..2937)
                     /locus_tag="ACHLCY_003731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370905.1"
                     /translation="MAFLRSVKSRRGIFAGGITYKGAWTQQTVEIYSWLACGAGFIDV
                     SLCQNLAS"
     gene            3008..3409
                     /gene="pliG"
                     /locus_tag="ACHLCY_003732"
     CDS             3008..3409
                     /gene="pliG"
                     /locus_tag="ACHLCY_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP0370906.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3512..3880)
                     /gene="ycgJ"
                     /locus_tag="ACHLCY_003733"
     CDS             complement(3512..3880)
                     /gene="ycgJ"
                     /locus_tag="ACHLCY_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP0370907.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDASGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4400..5095
                     /gene="minC"
                     /locus_tag="ACHLCY_003734"
     CDS             4400..5095
                     /gene="minC"
                     /locus_tag="ACHLCY_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP0370908.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5119..5931
                     /gene="minD"
                     /locus_tag="ACHLCY_003735"
     CDS             5119..5931
                     /gene="minD"
                     /locus_tag="ACHLCY_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP0370909.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5935..6201
                     /gene="minE"
                     /locus_tag="ACHLCY_003736"
     CDS             5935..6201
                     /gene="minE"
                     /locus_tag="ACHLCY_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP0370910.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6573..6917)
                     /locus_tag="ACHLCY_003737"
                     /pseudo
     CDS             complement(6573..6917)
                     /locus_tag="ACHLCY_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6952..7071)
                     /locus_tag="ACHLCY_003738"
     CDS             complement(6952..7071)
                     /locus_tag="ACHLCY_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0370911.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(7032..7217)
                     /gene="ymgJ"
                     /locus_tag="ACHLCY_003739"
     CDS             complement(7032..7217)
                     /gene="ymgJ"
                     /locus_tag="ACHLCY_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP0370912.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7318..7491
                     /gene="ymgI"
                     /locus_tag="ACHLCY_003740"
     CDS             7318..7491
                     /gene="ymgI"
                     /locus_tag="ACHLCY_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP0370913.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7493..7837
                     /gene="ymgG"
                     /locus_tag="ACHLCY_003741"
     CDS             7493..7837
                     /gene="ymgG"
                     /locus_tag="ACHLCY_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP0370914.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7841..8176
                     /gene="ymgD"
                     /locus_tag="ACHLCY_003742"
     CDS             7841..8176
                     /gene="ymgD"
                     /locus_tag="ACHLCY_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP0370915.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8233..8757)
                     /locus_tag="ACHLCY_003743"
     CDS             complement(8233..8757)
                     /locus_tag="ACHLCY_003743"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0370916.1"
                     /translation="MKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQ
                     PQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPF
                     VEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASVWGNVGVQLGDNG
                     YNDTAVMVGLKYKF"
     gene            complement(8754..10553)
                     /locus_tag="ACHLCY_003744"
     CDS             complement(8754..10553)
                     /locus_tag="ACHLCY_003744"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0370917.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKAHTLTPGRNIAGLKTR"
     gene            complement(10670..10879)
                     /locus_tag="ACHLCY_003745"
     CDS             complement(10670..10879)
                     /locus_tag="ACHLCY_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370918.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11279..11497)
                     /gene="ymgF"
                     /locus_tag="ACHLCY_003746"
     CDS             complement(11279..11497)
                     /gene="ymgF"
                     /locus_tag="ACHLCY_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP0370919.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(11629..13152)
                     /gene="pdeG"
                     /locus_tag="ACHLCY_003747"
     CDS             complement(11629..13152)
                     /gene="pdeG"
                     /locus_tag="ACHLCY_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0370920.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13484..13732)
                     /gene="ymgC"
                     /locus_tag="ACHLCY_003748"
     CDS             complement(13484..13732)
                     /gene="ymgC"
                     /locus_tag="ACHLCY_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP0370921.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3960 a         3197 c         2962 g         3680 t
ORIGIN      
        1 actatgaatt agatgaagta aaatttatta atagttgtaa aacaggagtt tcattacaat
       61 ttatatattt aaagaggcga atgattatga ctgaaatcgt tgcagataaa acggtagaag
      121 tagttaaaaa cgcaatcgaa accgcagatg gagcattaga tctttataat aaatatctcg
      181 atcaggtcat cccctggcag acctttgatg aaaccataaa agagttaagt cgctttaaac
      241 aggagtattc acaggcagcc tccgttttag tcggcgatat taaaacctta cttatggata
      301 gccaggataa gtattttgaa gcaacccaaa cagtgtatga atggtgtggt gttgcgacgc
      361 aattgctcgc agcgtatatt ttgctatttg atgagtacaa tgagaagaaa gcatccgccc
      421 agaaagacat tctcattaag gtactggatg acggcatcac gaagctgaat gaagcgcaaa
      481 aatccctgct ggtaagctca caaagtttca acaacgcttc cgggaaactg ctggcgttag
      541 atagccagtt aaccaatgat ttttcagaaa aaagcagcta tttccagtca caggtagata
      601 aaatcaggaa ggaagcgtat gccggtgccg cagccggtgt cgtcgccggt ccatttggat
      661 taatcatttc ctattctatt gctgcgggcg tagttgaagg gaaactgatt ccagaattga
      721 agaacaagtt aaaatctgtg cagaatttct ttaccaccct gtctaacacg gttaaacaag
      781 cgaataaaga tatcgatgcc gccaaattga aattaaccac cgaaatagcc gccatcgggg
      841 agataaaaac ggaaactgaa acaaccagat tctatgttga ttatgatgat ttaatgcttt
      901 ctttgctaaa agaagcggca aaaaaaatga ttaacacctg taatgagtat cagaaaagac
      961 acggtaaaaa gacactcttt gaggtacctg aagtctgata agcgattatt ctctccatgt
     1021 actcaaggta taaggtttat cacatttaaa aacacagacg ttttttaaag attgagagtt
     1081 acctgttgat aataaggtat cgggtaactc tcattatgac agagcgcttt caaaaacagg
     1141 gctacgaaat ggcgagcatc cagacgtata ttaagaaagc ccccataaac atctattaaa
     1201 tttatcactg cgcccagtca ggtttattta agatatgatc ctgccagtca atcacttctg
     1261 attctttcac tgcgatatga cgcacagaga tacgttcacc atgcattgcc gctttcgaac
     1321 ccgtaagtag cggatgccac gcaggtaaat ctttaccttc cgccagcaac cgataagcgc
     1381 aggtcattgg cagccattcg aatgttggca gattttcacg ggttaattta atgcagtcgg
     1441 gttcaaactc gaaacgacgt tcgtagttcc gacattgaca ggttttaata ttgagctggc
     1501 gacaggcgac gttagtgaag tagatttcgt cggtgtcttc atccatcagt ttatgcaggc
     1561 aacactgacc gcaaccatca cacaacgact cccattccgc atcgctcatt tcgtccaggg
     1621 ttttactttg ccagaaaggt acatcgctca tcaggtgttc cgccattacg ttaaaacgca
     1681 ccttataacc agtctggcac agcgatgcaa gttttgccgc cgctttcagg cggcaaaaag
     1741 tattacaaaa cgcgagttgc caaagaatgc ccatcgaaag tgactgtcag ctcatcaccg
     1801 ctttgcaacg ggccgacgcc atcaggcgtg cctgtcagca caacgtcacc ggccttgagg
     1861 gtaaaaaact tgctcatata agcgatcagc ggaacaattt tatggatcat gtccgcagtc
     1921 gtaccttgct gacgttgctc gccgtttacg ctcaggccca gcgttgtatt ttgcgggtcg
     1981 ccggtaaatt ccgccgcggg aataaacccg gaaagcggac aagagttatc aaacgcttta
     2041 gccttttccc acggctgccc ggctttcttc atttttccct gaacatcacg caatgtcaga
     2101 tcgagcgcca cgccataacc ggcaatggct ttgcggacat gctcttccgt agcctgacgc
     2161 agcgtcgcgc caatcaacac cgccagttcg acttcatgat gaactgaacc gaaatcggat
     2221 gggatcgcca atggctgccg cagatcgcac agtgccgttt ctggtttaat aaacagcact
     2281 ggctcttcgg gcactgcgct gcccatctct ttaatatgtt tggcatagtt actgccaaca
     2341 cagactactt tactcaccgg ataatccagc agcgcacctt gccagttgtg atgttgatac
     2401 ataacgttcc cctcacctta tcgtggatat gaaaacccgc gccgacaaag ataatgccac
     2461 cgccggatat cggttatttg ttagtgtcgt ctgttttctg ccccatgacg gaaagatgtt
     2521 gcttcagcaa atcttcgggt ggtggcggta actgcaaata gtaaccttgc tcggttaatg
     2581 ccagtttaac cttttcaata tcagcattga ccagtttctt ccgcccatcc aacggcagaa
     2641 tcatcgctaa ctgaggctga ccaaaacctt tcatcagttc ctcaggaaca cgcgaaaaat
     2701 cgtctttttt ttcgacatat aaataggtct ggtcacgctt gctgcttcga taaatcacac
     2761 aaaacatact tttactcttt aagatgccag gttttggcat aagctaacat caatgaaacc
     2821 cgcgccacaa gccagccagc tgtagatttc tacggtttgt tgagtccatg cccctttata
     2881 agtaatcccc ccggcaaaaa tgccgcgccg ggattttacc gaacgcagga aagccactgg
     2941 aattctcccg ttagggattg ttatattata acagttcatc gtactcattc tgaacaggag
     3001 actaccaatg aaaatcaaga gcatcagtaa ggctgtattg ctgtttgcac tgctaacctc
     3061 cacgagcttt gctgcgggta agaatgtcaa tgttgagttc agaaaaggcc atagcagcgc
     3121 tcagtactcc ggcgaaataa agggatacga ttacgataca tataccttct atgccaaaaa
     3181 aggtcagaaa gtgcatgtga gtatctctaa tgaaggcgct gatacttacc tcttcgggcc
     3241 aggaatcgat gattcagttg atttatccag atattcccca gaactcgata gtcacggaca
     3301 atactcactc cctgcttctg gcaaatatga gctgcgagtg ttgcaaaccc gaaacgacgc
     3361 ccgtaaaaac aaaactaaaa aatataacgt cgatattcaa attaagtaaa tgtcaaccga
     3421 cggggactct ccccgtcata aacgttaacg ccccactcct ctctgtcgct caagaggtta
     3481 atgacgaata gaacataatg taaacaagac atcactcacg acacataaat aacatctttg
     3541 tggtggtttg attgattttt ccactccgct taccatctac accaaaataa cgatcatcac
     3601 gacataattt ttctttaaca tcgcaaaata caccgttagc gaatgtaaat tcgctgggat
     3661 cgtagccttg taatgattgt aagttttcag cggctttttc gccgagatat ttccttgtta
     3721 actctgggga aatgccatct gagttcacac aaatatacgg gtcgcaaaca acgccgcacg
     3781 ccggagattg aagtccgcta gcgtcatgtc cagacgccat tgatgagaac atcatcccca
     3841 cacctgacaa tccgatataa ataatacgca tcatattcat agcacatcct gataatccaa
     3901 ccgtacaaca cttatgctca tttcgtcatc aatccgtaat caaaaccgct gacctggacg
     3961 agttacgatg acctgacaga agtatcagac gtcttctgcg gtttctatga tagagcaatt
     4021 ctgtggcgga tattaagata acgggtatct gttctttaat gatgccgcaa aaaatggaaa
     4081 gttaagaaag cctggcgtgg tgaaaaaata cacttgcctc aatactggat atttaaacag
     4141 gtctggagat gctgtcacga ttagggtgcg gaaaaaggaa gcgtaaataa gaagaatcaa
     4201 aagtacgacg gcaatgggtt gattgacaag ggtatttttt aagctatgaa tcagcgccat
     4261 ttatcacaga atagactttt actctgaata aatgggaggg tgacttgcct caatataatc
     4321 cagactataa catgccttat agtcttcgga acatcatcgc gcgctggcga tgattaatag
     4381 ctaattgagt aaggccagga tgtcaaacac gccaatcgag cttaaaggca gtagcttcac
     4441 tttatctgtg gttcatctgc atgaggcaga acctaaggtt atccatcagg cgctggaaga
     4501 caaaatcgct caggcccccg catttttaaa acatgccccc gttgtactca acgtcagtgc
     4561 actggaagac ccggtaaact ggtcagcgat gcataaggcg gtttcggcaa ccggtttgcg
     4621 ggttattggc gtaagtggct gcaaagatgc gcaacttaaa gccgaaattg aaaaaatggg
     4681 gctgcctatc ctgacggaag gaaaggaaaa agcgccacgt ccagctcccg caccgcaggc
     4741 tccagcgcaa aatacaacgc cggtcacaaa aacgcgttta atagataccc cggtgcgttc
     4801 cggtcagcgt atttatgctc cacaatgtga tctgattgtt acaagccacg ttagcgctgg
     4861 ggccgaattg attgccgatg gtaacattca tgtctatggc atgatgcgcg gtcgtgcgct
     4921 ggcaggggca agtggtgacc gggaaacgca aatattttgt acgaacctga tggcggaact
     4981 ggtgtccatc gcaggtgaat actggctgag tgatcaaatc ccagcagaat tttatggcaa
     5041 agcggcgcga ctgcagttag tcgaaaacgc tttgaccgtt caaccgttaa attgatccct
     5101 ttttaacaag gaatttctat ggcacgcatt attgttgtta cttcgggcaa agggggtgtt
     5161 ggtaagacaa cctccagcgc ggccatcgcc actggtttgg cccagaaggg aaagaaaact
     5221 gtcgtgatag attttgatat cggcctgcgt aatctcgacc tgattatggg ttgtgaacgc
     5281 cgggtcgttt acgatttcgt caacgtcatt cagggcgatg caacgctaaa tcaggcgtta
     5341 attaaagata agcgtactga aaatctctat attcttccgg catcgcaaac acgcgataaa
     5401 gatgccctca cccgtgaagg ggtcgccaaa gttcttgatg atctgaaagc gatggatttt
     5461 gaatttatcg tttgtgactc cccggcaggg attgaaaccg gtgcgttaat ggcactctat
     5521 tttgcagacg aagccattat taccaccaac ccggaagtct cctcagtacg cgactctgac
     5581 cgtattttag gcattctggc gtcgaaatca cgccgcgcag aaaatggcga agagcctatt
     5641 aaagagcacc tgctgttaac gcgctataac ccaggccgcg taagcagagg tgacatgctg
     5701 agcatggaag atgtgctgga gatcctgcgc atcaaactcg tcggcgtgat cccagaggat
     5761 caatcagtat tgcgcgcctc taaccagggt gaaccggtca ttctcgacat taacgccgat
     5821 gcgggtaaag cctacgcaga taccgtagaa cgtctgttgg gagaagaacg tcctttccgc
     5881 ttcattgaag aagagaagaa aggcttcctc aaacgcttgt tcggaggata agttatggca
     5941 ttactcgatt tctttctctc gcggaagaaa aacacagcca acattgcaaa agaacggctg
     6001 cagattattg ttgctgaacg ccgtcgcagc gatgcagaac cgcattatct gccgcagttg
     6061 cgtaaagata ttcttgaggt catttgtaaa tacgtacaaa ttgatcctga gatggtaacc
     6121 gtacagcttg agcaaaaaga tggcgatatt tctattcttg agctgaacgt gaccttaccg
     6181 gaagcagaag agctgaaata agcccgctgt aaaaacgcat ttatcttcaa ggcagagtca
     6241 tttctgcctt gagtttttca tccctctcat ccacgttgtg gtaaagcggc gagtattctt
     6301 gctgatactc ctcattgcta tttccacctc cccctttaca ccttaaggct gttcccacgc
     6361 acagaacaaa atgtttatgc ctggttgagt aaataacctc attgttagtg tggatacact
     6421 gggggatgat aaccccgcaa tttcattatt cattgttctc ccataggatg aggtttcccg
     6481 ccgtaactgg cgggcttttt ttgccccaga attttccctt ttagccccct gtaaacgaaa
     6541 actgcgccga agcgcagttt aatcgttgat ggtcagaacg accagttcac accagccacc
     6601 gcattccacg gggattccac accggcacca tggctatacc ccaccccgaa atgcccgctt
     6661 aacgtactgc tgaatgaggc tttaatacct gcctggtata ttccacgtct gcccgacaaa
     6721 tcattgacaa aattaccgtc actattcact ttcacccgat tatcatcgac aaattctttg
     6781 cgcacagccg ccttcaggca gggttcaatt tccataccat tccccagacg catgttgtaa
     6841 ctcagcgttg cacccagttc acgatatata ctgcgggtat cgactgattt cgattccatg
     6901 ccattggata aatgatagta gcgtctaagt gacctaagct ctgctgcaac atcagcccgc
     6961 aggcaccaga ccaggggatt catcctgaag agacagcgca ggtgtattgt gttcaccgct
     7021 caccaaagac atcatgatga aatgatgata tcccgcataa gaaagaggca ttttttacac
     7081 gcagtgcgct gaagtgtagt tggataaaaa atcactcttt tcagataata ctgtcgtacg
     7141 cttatctttc gacaatgagg ccgtcggcaa aataaaatga ctaatgtaat cgttgctgat
     7201 gaatatcttc tgctcacata aaaatcacac aataatattg ataaatcaga ttatcttacc
     7261 tttacaacat tgcgttaaca gtcaatgaca cttcttgaat aaaaatattg tcaatcaatg
     7321 agttattcct catttaagat tattctcatt cacgtaaaaa acataattcc tatcatcaca
     7381 gccacgctca ttttgaatta tctgaataat agcgagcgtt ctttagttaa gcagatctta
     7441 attgaagacg aaattattgt gtgtgcaact tacttaatac cggacatcta agttgaagaa
     7501 aaaaatactt gccttcgggc ttatctcagc attgttctgc tccacgccag cgatggcgga
     7561 tatgaatcgc accacgaaag gtgcattgct aggcgcaggc gttggtttac ttactggcaa
     7621 tggtgttaat ggcgtactga aaggtgcagc tgttggcgct ggtgttggtg cagtaacaga
     7681 aaaaggccgc gacggtaaaa atgcgcgtaa aggtgcaaaa gtaggtgccg ccgttggtgc
     7741 tgtcactggc gttctgaccg gcaatggcct cgaaggggcg attaaaggtg ccgtgattgg
     7801 cggtacgggt ggtgcaattc tgggtaaaat gaaataagga atgatgatga aaaaatttgc
     7861 tttactggcg ggcctgtttg ttttcgcacc aatgacctgg gcgcaggact acaatattaa
     7921 aaacggtttg ccgtcagaaa cctatattac ttgcgcagaa gccaacgaaa tggcaaaaac
     7981 tgacagcgca caggttgcag aaattgttgc ggtaatgggt aatgccagcg ttgccagccg
     8041 tgatttaaaa attgagcaat ctccggaact gagcgccaaa gtggtagaaa aactgaatca
     8101 ggtttgcgct aaagacccgc aaatgctgtt gatcacggca attgatgaca ccatgcgtgc
     8161 tatcggcaaa aaataatatt tcatatgtac cgcgtcgatg aatacgacgc ggttttttag
     8221 ctaacggcgg gatcagaact tatatttcag gcccaccatc actgcggtgt cattgtagcc
     8281 attatcaccc agctgcacgc ccacattccc ccagacgctg gccgctggat tcaggttgcc
     8341 atttacgcca agtttaattt cccccaaatt actcgcccca tcttgttcga ctttcacacc
     8401 gttcattgag acagcaaaat ctttactgtt atggatccag ttcatttcaa caaaaggttc
     8461 aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta cgaataaagg tgcggaaacc
     8521 aagtcgggtc tggatgttgt tatttgcgtc attctcaata cgtgagccat tggcttcggt
     8581 gtgcgcatta tgatcgacgc ccatccagat agcctgtgcc tgtggctgga atatccacgt
     8641 gtatttcgcc gcttccagac caaaggattt attcagggca aagccgtaac ccgcttccag
     8701 cgagacggtt gcgcctttcg cactatagga ttcataggct aattcatccc ctttcaccga
     8761 gtttttaaac cagctatatt gcgcccaggc gtcaatgtat gcgctttttt actgatgtca
     8821 tcggcaaacc aagtggcata cagccctgcg caatagcctc tgacactccc tttcgaacga
     8881 taatccgaca cgctggaatg agttgagttg tagtcgcggg cataaccagc catcactccc
     8941 aaacgccaac tgtcactgtc ggtaaaacca ccggttaaca gctcggcccc taactgcgag
     9001 acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt cacgccaggc attatgcccg
     9061 ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt cagttacagg atcgatgtag
     9121 cgcatttcac ccgcacggtc gttcagacgc ataacaaaaa gagtgttagc ggctgccagg
     9181 ttggcgacgt aacttccagc ttcgaggcgc aggacggggg taggcgcaac agtaggcgct
     9241 gaacttcctc cggagtttgt ttcaccgccg ctcgttgcct ggaagttagt cagataccag
     9301 tttttattgg tcgcttcggt attacctcgg gttaatgaat attcgtaggc accggcgaaa
     9361 atcctcgaat ccttaataaa ttccccattt gattccccct caacgctgat aatctctatc
     9421 ccatttaacg tgtaagctcc gctgcctcct gcattggtaa ctttaactcg ggttgttcca
     9481 gaggtatttc cattaataac gactttatcc gtagctgaat tatcatcacc caattcagtt
     9541 ctgagatgca atacgccatt attaccaacg taattttcag ttattgttaa tcttgttggt
     9601 tcgacatccc gtccatctgc ccgggaaata tagaccgtac tattatcaac cgtgaggttg
     9661 ctcaccgttg agtcagcttt aatatcccag gtactattat ctgaaagcga cagataggta
     9721 tgagtgttaa catctgttga aatattagct gaacccgtta tttttgaatt agttgcatcg
     9781 acggtcaaat gacctttaat attattgata aaaacatctg cagccgatga tacctcacta
     9841 tccgtaatgg tcacgttact cgtctcattt ttatggtaga ggatttcatt tgctgaccct
     9901 agtgagctac cagataaaat atctaccgtt gcttcctgac catttatgga agactccagc
     9961 acaattccag acccctgatg cgcgactgca gatgtatttt tcagcgtcac cgtgccgcca
    10021 ttataaattt tagcagtcgc ggcgtcagga cccgttgtat aaatttctcc ctggttaaat
    10081 gaaactgtcc cctggcgggc agcgatggca tgagcattgt ccccatgcgt ggtgatatca
    10141 acatcggtta atgtaacgct ggcattggta taatcaagcg tgtcaccagc cttgacgaca
    10201 gtgccattag cgtacgcggc ataactttct gttccgtggg ttgtgatctc cccttttgcc
    10261 atatccagtt ttgctatggc attattttca ttacttcctg tatcggtagt actttcttca
    10321 tcaagagaag atacacacgc acgcgcgctt ccgggagatg cacacgcaag aacgccgact
    10381 gcatgattac ccgcagtaac aataaatgta tcatctccgt cagaaaaaga gggggtaacc
    10441 gaaccgtcag agttgactct tattatggtg tcagtattca ggtttccact cgtttgactt
    10501 agcaaaccaa ttgagtaatc ccccgttatg acaatcgtgt ttttaccctg caaaataaca
    10561 gcaccgccaa attcacttac gattgcgcta gctgttaatg ctgtgcttag aggactattg
    10621 tcagaaggat ataaaccgcc cgttgttaca atgacgccat tgttaatggt taacttgcta
    10681 ccctctcctg caacggatac ccgtaacttc cattacccgt tgttataact agcgcattat
    10741 caatatcttt tacttttcca tcttttattg ccacagcatg tttatattca tcgtgctgaa
    10801 taaactgttc agcatatgca ttacccaccg ccagagcaat aagtcccaaa ctaaacccgg
    10861 agagtttttt cagtttcata gattaaccat ccattcattg gattaacaaa cattcctggt
    10921 caaataacct atgcgttcag attaccaaaa cggagggtaa agacaaaaac aaaagcacaa
    10981 aaaatttaaa tagataaaca gcgacaatat ataaatgata aataagtcgg cattatcatt
    11041 tttcacctta ttttcatgac attgatcact ttgaggtgac gattttgttt aacaaataac
    11101 tcttcgacat cacaatcatt gatagttgca ataaaaatta tttcaatgac atataaaaac
    11161 aactgtgata acaccagggt tgacaactat ttgcgcaata attcctttac ttcaatgatc
    11221 caggcattca ctgccagcaa cccattcagc cgcatactga cagtgagaaa ctgaaagatt
    11281 attgctgatc cctgtggctg acgagtcttt cccttaatcg attaaccaga ttgatcgcca
    11341 gccaaacaag ggcattaagc aaatttatcg tcacaagcac ttctcccagc ccggtactgg
    11401 gttttgcaat aaggatgacc aggagggtga tcagcataaa tgcaatggaa aagatgatat
    11461 agagtgtgaa ataatccaga ttattactat tgttcatgct actaatggca atcctgtcaa
    11521 aactttcaaa caattatagc aaacaaaata atgaacaata gtaacaagag gcaacgagct
    11581 aattagctgt ctataattac accgcactat ggccttactg atcaattttc actcaaccac
    11641 aaccttcacc ttcggtttag aaaggataat tttgaccagg tcgatgtatg taacaggttt
    11701 atagaaataa taaccctgct gaaatgtgat attattttgg ttcagatagt caagttgttc
    11761 tttcgtttcg acaccttcag cgacgatact gatcgaaagt ttacgcgcca gatccaatac
    11821 acaatccaga attcgggttg attctgggtc tgcgtttacg cggccaacga aactgtgatc
    11881 aattttgata taatcaatat gcaggtcatg aagataagaa agccctgagt agccagtacc
    11941 gaaatcatcc agcgcgatga caaaaccatt ttcatgcaga atgttcaacc gctgaaccag
    12001 actttcatca acattcaatg gctcacgctc ggtgacttcc agaacaaggt ttaaatcgcg
    12061 gcgggtaaaa ctgtcacgga aatttaaaca ctcgtcgaca aacgtcggcg aaataatatg
    12121 cgaagcgcta aaattaattc caatatgaaa accttccggc aatttactcg cgatagcgtt
    12181 catctgtctg gcaacctgat tcatcaggct ttgcgtaagc ggaacgatta atccggattt
    12241 ttcagcaagt ggaataaatg ccgcgggtga tatatatcca ccgtgaggtt gtttccagcg
    12301 ggctaacacc tcaactcccc gcaatgtccc ttcccgacca tttaccacag gttggtaaaa
    12361 agggacgatc tccccattat ttattgcccg tcgcaggatc tcttcaggcg ttgcgctttt
    12421 attgaaatat ctatcaagca ggaaggcagc ggcgcaggcg attaacagaa taaatattaa
    12481 aatcccaaag cctttatcga taagtctgta gaagctaaag agtggcggcg ggttaaagat
    12541 aatagtgaaa tgatattttg tggagtgaac cttctcggta tagggataat gtccattaag
    12601 cgtcatcaca tcacccgttg gtccaataat gtcatccgcc acgcgtaata cataccttac
    12661 ccctttcaag ggtacattaa gtgccccacg aatatgctga tcgcttatcg taaccaaaat
    12721 gcgcgtatct gcaaattggt tctgatagag caggataggt aatctattaa cggtgtcgat
    12781 tgctggagcc aacagtaaat tactgtccgg gaaaacagga atacgagaca acaggacccg
    12841 attcccaggc agggatgtac accagactat tccctgtttg agaatgatga ttgtgcgcag
    12901 gtgaggtttc agagctgctt cagtgccaag ctgatattgc tcctcgaggt tgcattcctt
    12961 cccggcaatg ttaaccgctg tttgagtcgc ttgtgacgct tcttcaagta tatgattgat
    13021 attgttggcg gcatatctcg ctcccgccag gtactctgct tttgcggaat accagagctg
    13081 gatgttcaaa atagcgacac cggtaataaa caagcatatc gcaacgagga tgggtatgag
    13141 tgtattgcgc atttgcttgt cccggccaaa gggtttaata catgaaaatg ccattcattc
    13201 gctcgcgctg gcatctacag agagcatggt agagagcata agaaatatcc acgccgaaga
    13261 taaaattttc tccatcttga tctaacccat taattattga gcataatgta ggcagtacaa
    13321 aataagttag gcgggatatc aggcgtcaag aatggagagc gagaactctc cattcttgac
    13381 acctgatatt gcggacataa taagaaagca taacgcctga aatgctcact ttgcatcagc
    13441 atggtgatac agctgatgtt tattctaaaa ccttactcaa gttctaagag agcacggatt
    13501 ccctgtcatt aataatgata tccgttagtg tagtcttatg catttcaatg tactgcaata
    13561 tttgcagata gagatcagaa agaatcacac tggtttcatc ttttaaacgc aagcgtaaag
    13621 cctgatcaat aatatgacta tcaacatacc catgcgtcat gaacacctcc ctctcgagat
    13681 ttttaaacaa agcacattga aaaataaatc tctctgggat tgaattattc atgtcagcct
    13741 cgtagaaata ttaacccaat aaaattatgc tgtgtatagt ctaaagcgac agacaaaaa
//
LOCUS       DAZGTR010000112        13245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGTR010000112 DAZGTR010000000
VERSION     DAZGTR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.114"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(11..763)
                     /gene="tcyN"
                     /locus_tag="ACHLCY_003749"
     CDS             complement(11..763)
                     /gene="tcyN"
                     /locus_tag="ACHLCY_003749"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP0370922.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(760..1428)
                     /gene="tcyL"
                     /locus_tag="ACHLCY_003750"
     CDS             complement(760..1428)
                     /gene="tcyL"
                     /locus_tag="ACHLCY_003750"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP0370923.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1443..2429)
                     /gene="dcyD"
                     /locus_tag="ACHLCY_003751"
     CDS             complement(1443..2429)
                     /gene="dcyD"
                     /locus_tag="ACHLCY_003751"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP0370924.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGC
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2534..3334)
                     /gene="tcyJ"
                     /locus_tag="ACHLCY_003752"
     CDS             complement(2534..3334)
                     /gene="tcyJ"
                     /locus_tag="ACHLCY_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0370925.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVTLRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3422..3973)
                     /gene="fliZ"
                     /locus_tag="ACHLCY_003753"
     CDS             complement(3422..3973)
                     /gene="fliZ"
                     /locus_tag="ACHLCY_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP0370926.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4019..4738)
                     /gene="fliA"
                     /locus_tag="ACHLCY_003754"
     CDS             complement(4019..4738)
                     /gene="fliA"
                     /locus_tag="ACHLCY_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP0370927.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4903..6381)
                     /locus_tag="ACHLCY_003755"
     CDS             complement(4903..6381)
                     /locus_tag="ACHLCY_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFP0370928.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYDVGGKTYTVNVESGAVKNDA
                     NKDVFVSAADGSLTTSSDTKVSGESIDATELAKLAIKLADKGSIEYKGITFTNNTGAE
                     LDANGKGVLTANIDGQDVQFTIDSNAPTGAGATITTDTAVYKNSAGQFTTTKVENKAA
                     TLSDLDLNAAKKTGSTLVVNGATYNVSADGKTVTDTTPGAPKVMYLSKSEGGSPILVN
                     EDAAKSLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSR
                     IEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6636..8048
                     /gene="fliD"
                     /locus_tag="ACHLCY_003756"
     CDS             6636..8048
                     /gene="fliD"
                     /locus_tag="ACHLCY_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0370929.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGDYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLINTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8063..8473
                     /gene="fliS"
                     /locus_tag="ACHLCY_003757"
     CDS             8063..8473
                     /gene="fliS"
                     /locus_tag="ACHLCY_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0370930.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVETLMRNIADAWKESLLSPSLIQDPV"
     gene            8473..8838
                     /gene="fliT"
                     /locus_tag="ACHLCY_003758"
     CDS             8473..8838
                     /gene="fliT"
                     /locus_tag="ACHLCY_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP0370931.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8916..10403
                     /gene="amyA"
                     /locus_tag="ACHLCY_003759"
     CDS             8916..10403
                     /gene="amyA"
                     /locus_tag="ACHLCY_003759"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0370932.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10437..10850)
                     /gene="yedD"
                     /locus_tag="ACHLCY_003760"
     CDS             complement(10437..10850)
                     /gene="yedD"
                     /locus_tag="ACHLCY_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP0370933.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVNTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11037..12242
                     /gene="yedE"
                     /locus_tag="ACHLCY_003761"
     CDS             11037..12242
                     /gene="yedE"
                     /locus_tag="ACHLCY_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP0370934.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQLCFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12239..12472
                     /gene="yedF"
                     /locus_tag="ACHLCY_003762"
     CDS             12239..12472
                     /gene="yedF"
                     /locus_tag="ACHLCY_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP0370935.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVMSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12579..>13245
                     /gene="yedK"
                     /locus_tag="ACHLCY_003763"
     CDS             12579..>13245
                     /gene="yedK"
                     /locus_tag="ACHLCY_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP0370936.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
BASE COUNT         3299 a         3326 c         3264 g         3356 t
ORIGIN      
        1 ttttttctta ttattgcagc agaaacttct cgaggaactg gcgggtgcga ggctgctggg
       61 gatcggcaaa taacgctttt gctgccccct gctcgactat ccgcccctgg tccataaaga
      121 tcgcccggtc cgcaacatcc cgggcaaagc tcatttcgtg tgtcacaatc accatcgtgc
      181 gcttttcctg cgccagctgg cggatggtgt tcaggacttc gcccaccagc tctggatcca
      241 gcgccgacgt tggctcgtca aacaaaatca cctcaggacg cattgccagt gcacgcgcaa
      301 tcgcaacacg ctgctgttga ccgccagaca aacgacgtgg atagctggtt tctttaccgg
      361 ccagcccaac tttcgccagc agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc
      421 ctttgacgat caccggccct tcaataatgt tttccagcac cgtacgatgc ggaaacaaat
      481 taaagttctg gaagacaaac ccgacgtgct gacgcaactg gcgaatcagc gatttttgct
      541 gacttaatga acgtgcagta tcaatagtga tatcgccaac ggtgatcgtt cccgcttccg
      601 gttgttccag cagatttatg ctgcgtagca acgtggtttt gccggaacca ctcggaccga
      661 taattgccac cacttcgcca ggctttacct cgaggtcgat accgtgcagc accgtctgac
      721 cgtgaaattt tttcaccagg ttcttaactt caatggcact cattttggtt ctctctcctg
      781 gcgattaagt tgattctcaa aatggttctg caacgtcgat aatactgtcg ccatgatcca
      841 gtagatgagc gaagccgcca ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa
      901 ctgcgcctga cggaacagct ccggcacctg gattgtcgcg gccagcgacg tatcttttac
      961 cagactgata aagctgttcg acagcggcgg caacgccacc cgcgccgcct gtggcaaaat
     1021 cgcgcgacgc atggtctgcc acggcgtcat accaatactg gcagcggctt cccactgacc
     1081 tttatcaata gaagaaatgg cagcgcgcag cgtttcggcg gcataggcgg cagtgttcag
     1141 cgacagacca atcattgctg acggaatggg atctaattcg ataccaaact gcggcaggcc
     1201 gtaatagatc ataaacagtt gtgcgatgag cggcgtacca cgaaaaatag agatataaaa
     1261 acgcgccagc cagcgcacgg gccagattgg cgacaggcgc atcagcgcga gaataaaccc
     1321 gagtagtaag ccaaaaaaca tgccgccgat actgagttgc agggtatacc cagccccttt
     1381 caacaggaac ggcaaggaat caataaccag ttgtatactt tcttgcatga gcgttcccgg
     1441 agctaaacgt ggggatgata ggcgaacagc gcaggcgcgc cgccggtatg aataaacaga
     1501 atcggccctt catctttgaa gcgtttctga ctgataccgt caatcagccc cgccatcgct
     1561 tttccggtat acacaggatc aagcagaatg ccttccagac gcgccagcaa tttcactgct
     1621 tccatgcctt cgtcgttcgg cacgccgtag ccaggtgcaa aatagtcatc ccagagtaaa
     1681 atttccgctg atgcggtcag ctccagttct ttcgcaatcg cctgttgtag gttaaccact
     1741 ttcggcaatt gatcggcaac ggaacgcgac acggtcacgc caatcagttc gctttcaggc
     1801 atcaggtgtt ccagcccaac agccaatcca gcgtgagttc cggcactgcc cgatgcgacc
     1861 accaccgacg aaatattaac cgccccttca cactgttgcg cgatttccag cgcactctcc
     1921 acataaccca gcgcgcccag agcattagaa cagccaaccg gaatgacata cgggcgaaag
     1981 ccttgtgctt cgactcgcgt cgccagctct tccagttggg cattgggatc ggtcagtgcg
     2041 tcgcacattt caatctgggt attgaacaga tccagcaaca aacgattgcc gttggttaaa
     2101 tagttttctg cggttgtgcc aataggattt tccagcagcg ccacgcagtg cagaccgagt
     2161 ttcgccgcga ctgcggcagt ctggcgcaca tggttagact ggatcgcgcc ggcagtaatc
     2221 agcgtatcgg caccttcgcg cagggcatct gccgcgagaa attccagctt acgtaattta
     2281 ttgccgccca ttgccatggg tgtgacgtca tcccgtttga tgaaaatttc ccgtccaaga
     2341 taatcagaaa agcgcggcag atattcgagc ggcgttggcg cgccgataaa ctccaaccgt
     2401 ggaaaacggg ttaaattatg cagtggcata acagcctccg atgtgtgttg ttgtgatttt
     2461 cttattatgc acgctgaaaa cgcgtaaata aaaaaggcgc tagtgaaagc gccctttttt
     2521 gtcattatgc tgattatttg gtcacatcag caccaaacca tttttcggaa agagcttgca
     2581 gagtgccgtc tttttgcatt tccgcaattg catcattcac cgctttcagc aggtcttcat
     2641 ttcctttacg cagcgtcacg ccagactcct gacgggagaa tgcttcaccg gttactgcca
     2701 gcgtatcgtt ggttttcttc accagatcca gcgccgccag acgatcaacg aggatcgcat
     2761 cgatacggcc tacgcgcaga tcctgatatt tggtcgggtc atcatcatag gtacgcacat
     2821 cgacgccctg aacattctgc cgcagccact cttcatagtt ggtgcccaga ccgacaccca
     2881 cttttttgcc tttcagatca gcagccgttt taatggtgcc ttcgttacct tttttcacca
     2941 gcgcctgaat accggaaatg gtgtaagggg tagagaaatc gtattttttc ttgcgctcgt
     3001 cagaaatggt gacctgatta atcaccacat caatgcgttt agagtccagc gacgccagca
     3061 taccgtccca tttggtcggt tttagtgacg cctcaacgcc aagatgtttt gccagctgtt
     3121 gggcaaattc cacttcaaaa ccggttaatt tgccgtcatc tccctgaaaa ctgaacggcg
     3181 gataagtgcc ttccagccct accagcagcg tgccgcgctc tttaacttta ttaagcagac
     3241 cttcatctgc aaaactttta acgctcatgc ccgcaaccag cgctacggcc atcacaccca
     3301 tcaatgcctg acgtcccaga tgtgctaatt tcatatttac cccgaatgtt gttatgtctg
     3361 tttgcagtgt agagccattg tttgtaaaca caaaaacaac tccgctacat cttattctta
     3421 tttaatatat atcagaagca ggcaggctgg aggatttctg cacaagtccg gtacacgttt
     3481 gacgctgata gtgttgatac ttccgtaatg cgatgcggta attgttggtg ctggtggtgg
     3541 gcagccacgg ggcgaggatt tcatcaagga aaccgtcctg cagcggatcg agggaaatat
     3601 tttgctcgtg tagatgattc cccaaacggc gcagacgaac gacatattca cgcaccgtac
     3661 cagggctcat ttcagtttgc tcaaaaagaa attgcttaaa gccgataata tcgaaaaaat
     3721 cactctgcgt tttgcaatgt aaatcaccgc agaatcggca caatgccgcc catgattttt
     3781 gttccacttg ccagccattt tcatcaagca gcatgtccag gcgggaaatc tcgattttat
     3841 tcactatttt gccgtcgcga acgagggtaa tgcggtcgag taatttacgg caatgcgcgc
     3901 aatgggtctg gctgtgttta aagtctttaa gatagcggct taatggccgt cttttcaggt
     3961 gctgcaccat cattaagaac tcctggtagt caaaattaaa gtgcggcatt tactgacgtt
     4021 ataacttacc cagtttagtg cgtaaccgtt taatggcctg gctgtgtaac tgactgaccc
     4081 gcgattcccc gacctccagc accgcgccaa tctctttgag attcagctct tcctggtaat
     4141 agagggttaa taccagtttt tcgcgctccg gcaacgtttc gatggcttcc atcacccgct
     4201 ggcgcagatt actgtccagc agttgttgta gcgggttttc tcgctgatga tcatcagtaa
     4261 ccagttcgat gctatcgccg tgctcttcgc gccactcatc gtaggagaag agctggctgt
     4321 tattggtgtc gagcaacatt tggcgataat cggcaatatc gatccctaaa cgttccgcta
     4381 cctcagtttc cgtggcgttg cggccaagtt cctgctccag ttgccctatt gcctgtgcca
     4441 cttcacgcgc gttgcgtcgc acgctgcgcg gcacccagtc acggctgcga agttcatcca
     4501 gcatagcgcc acggatacgc tgcactgcgt aagttgtaaa tgccgttcct tgtagggcgt
     4561 catagcgttc gacggcatta agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt
     4621 ccacgctcgc gggcagtcga acctgcaggc gcaatgcttc gtgacgcacc agcgggacat
     4681 aacgctgcca cagcgagtgt ttatccatta caccttcagc ggtatagagt gaattcacga
     4741 taaacagccc tgcgttatat gagttatcgg catgattatc cgtttctgca gggtttttaa
     4801 tcggacgatt agtgggtgaa atgaggggtt atttgggggt taccggtaaa ttgcggacag
     4861 aaaaaacccc gccggtggcg gggtttgagc gataagtgta aattaacgca gcagagacag
     4921 tacgttctgc gtggtctggt tagcctgcgc cagaacagag gtacccgctt gttgcaggat
     4981 ctgcgcacga gacatgttag acacttcggt cgcgtagtca gcatcttcga tacggctacg
     5041 ggcagaagac aggttgttta cggtgttgcc aaggttggtg atggcagagt cgaaacggtt
     5101 ttgtactgca ccgaggtcag aacgcagatt gtcaacttta gccaatgcct tgtcgatagt
     5161 ttcgagcggg ttggtggtag attgcaacga ttttgctgca tcttcgttta ccagaatcgg
     5221 gctaccacct tctgatttgc tcagatacat cactttaggg gcaccaggag tagtatcagt
     5281 taccgtttta ccatctgcgc tgacattgta ggtggcgcca tttacaacta aagtgctacc
     5341 tgttttcttg gctgcattaa gatccagatc agagagtgtt gcggctttat tttccacttt
     5401 tgtagtggtg aactggcccg cactgttttt gtaaacagct gtgtctgtag ttattgttgc
     5461 gccggcaccc gtgggtgcat tactgtcaat agtaaattga acatcttgac catcaatatt
     5521 tgcggtcaaa acacctttac cattagcatc aagctctgcg ccagtgttgt tagtaaatgt
     5581 aatgcccttg tattcaatgg agcctttgtc agctaatttt attgcaagtt tcgctagttc
     5641 tgttgcatca atactttcac cggatacttt agtatcacta ctggtcgtca gcgatccatc
     5701 agctgcgctt acaaaaacat ctttattagc atcattctta accgcgccgc tctccacatt
     5761 cacggtataa gttttaccgc caacatcata attatcagta cctgtcgctt taaatttaga
     5821 aatcaggtca ctgcctgttg ctttctgcgc gccatcgata ttaaaaccgt ccaggccgag
     5881 agttttcgca tcaatttttg ccagattgat agtgatggtt tcaccatcat tagcaccaac
     5941 ctgaattttc atttcattat tttcagcaag gactttcacg ccgttaaact gagtttgctc
     6001 agatacacgg tcaatttctt ccagacgttg agtaatttca gcctggatag aagaaagatc
     6061 gctgtcagag ttagtaccgt tagttgcctg aacagaaagt tcacgaatac gctgcaggtt
     6121 gttgttaatt tcattcagcg caccttcagt ggtctgcgca acagaaatac catcatttgc
     6181 gttacgggaa gcctgggtca gacctttaat atttgccgta aaacggttag caatcgcctg
     6241 acctgctgca tcgtcttttg cgctgttaat acgcagacca gaagacagac gctcaatagc
     6301 agagctaaga gaagactgag atttgttcag gttattctgg gtcaacagcg acaggctgtt
     6361 tgtattaatg acttgtgcca tgatcttttc cttatcaatt acaacttgat gttattgggc
     6421 tgttgcccac ggtttctcac cgtacccctt gtatcggcac ctgaatttcg aactttagaa
     6481 aattttagtt tctctcctgt tttttcttaa cggctgaaat atccgtaata tacaactcta
     6541 ctctgcgcat catcttcgtg aatcaactat taaactttgc gcacaaattg ccgataaccc
     6601 gcttatctac tgtttgcaat caaaaggaat taggtatggc ttcatttaca tctcttggcg
     6661 ttggttcgaa tctgccgctg gatacattgc taaacaacct gaccatcgct gaaaaaaaac
     6721 gcttaaaccc aattactcag caacagagtg acaataccgc acgtctgaca gcctatggca
     6781 cattaaaaag tgcattagaa aaattccaga ctgcaaatac tgcgttaaat aaagccgatc
     6841 tgttcaaaag cactaccgtt accagtagca atgaagacct taaagtttcc accgaagcag
     6901 gtgctgcccc cggtatctat accatcagcg taacccaact ggcgcaggcg caatctttac
     6961 gaactgattc cccgacaatc attgcatcaa caaaagatgc actgggtgat gagtcatcag
     7021 atacacgcac aataaaaatt acccaagacg gccgtaaaga gccgctggaa atcaagctca
     7081 ataaagatca gacgtcattg gatgagatca gcaaagccat taacgatgcc gatagtggaa
     7141 tttccgctag tatcgttaaa gtcaaagatg gtgactatca attggtattg accgccagtg
     7201 aaggtctggc caataaaatg accatatctg ttgagggtga cagtaaatta aatgatctgc
     7261 tggcttatga cagcaagacc aacaccggga atatgaagga actggttaac gcgcaaaatg
     7321 cacagctaaa cgttaacggt atcgatattg aacgttcaag taacaaaatt accgatgctc
     7381 ctcagggagt gactctcgat ttgaccaaaa aagtcactga cgtccgggtc acggtgacaa
     7441 aaagcaacga taaagcaacc gaagcaatta aaggctgggt ggattcatat aactccctca
     7501 ttaacacctt taacaccctg acaaaatata aagaagtaga cccaggtgct gaagcacaag
     7561 ataaaaataa tggtgcactt ctcggagaca gcgttgtacg tacaattcaa agcggcatcc
     7621 gcgcacaatt tgcaaacggt gccagcgacg gtgccttcaa aacattaaac gagatcggga
     7681 ttaagcagga cggcacgacg ggtaagctga aaattgatga tgataaactc aaaaaagtcc
     7741 tgaatgaaaa tacagcatcc gtacgtgaat tgctggtagg cgatggtaaa gaaacaggaa
     7801 ttaccaccaa aattgccacc gaggtaaaag gttaccttgc cgatgatggc attattgata
     7861 gcgcacagga cagtattaac gccacactga aaaaactgac caaacagtat ctatccgtca
     7921 gtgccagcat tgatgacacc gtggcgcgtt acacggcaca gtttacgcaa ttagatacca
     7981 tgatgagcaa actgaataac accagtacct acctcagcca acaatttact gcaatgagta
     8041 attcctgatc acggaggtca gcatgtatac cgcaagcggc tcaaaagcct atgcacaaat
     8101 tggcgtcgaa agcgccgtaa tgagcgccag tcaacagcag ctggtcacca tgctatttga
     8161 tggagtgctg agcgcactgg ttagagcgcg cctgtttatg caggacaaca atcaacaagg
     8221 caaaggcgtc tcattgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct
     8281 tgatgaagag agcaaagacg aattaaccca aaacttgatt gctctttata gctatatggt
     8341 caggcgtttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aagtggaaac
     8401 attaatgcgc aatattgccg atgcctggaa agagtcgtta ctctcccctt ctttgattca
     8461 ggacccagtc tgatgaacca tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa
     8521 aaaagccagc tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgccagc
     8581 gaaatggcgt atgttaatgc ggtgcaggag attgcacatt tgactgaaga gattgacccg
     8641 tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga caacgaaagc
     8701 aaggtaaagc agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca
     8761 tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg
     8821 caggataacc tcttttgaat ctgaatgagt cgatggctcg cgaataatcc gattacggct
     8881 acgcttctaa tgttcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat
     8941 gttttcactg gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgccg
     9001 acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat
     9061 cgggcgggta ttcggtcggc tacgactcct atgatttatt tgatttaggc gagtttgatc
     9121 agaaaggcag catccccact aaatatggcg ataaagcaca actgctggcc gccattgatg
     9181 ctctgaaacg taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg
     9241 cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc tgatgaccgt acgcaaattg
     9301 acgaagaaat cattgagtgt gaaggctgga cgcgttacac cttccccgct cgtgccgggc
     9361 aatactcgca gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaacc
     9421 ctgatgaaga tggcattttt aaaattgtta acgactacac cggcgaaggc tggaacgatc
     9481 aggttgatga tgaattaggt aatttcgatt atctgatggg cgagaatatt gattttcgca
     9541 atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat
     9601 gcgacggttt tcgtcttgat gcggtcaaac atattccggc gtggttttat aaagagtgga
     9661 tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggcagag tactggtcgc
     9721 atgaagtcga taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgttcg
     9781 atgcgccgct gcagatgaaa ttccatgaag catcgcgcat ggggcgtgac tacgacatga
     9841 cgcagatttt cacgggtaca ttagtggaag ccgatccttt ccacgccgtg acgctcgttg
     9901 ccaatcacga cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg tggtttaaac
     9961 cgctggcgta tgccttaatt ttgttgcggg aaaatggcgt tccttcggta ttctatccgg
    10021 acctctacgg tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata
    10081 tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg
    10141 tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt ggcactgacg
    10201 aatatcccgg ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa accattaatc
    10261 tgggagagaa ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagtg
    10321 tagtaaccga cgaaaacggt gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt
    10381 gggttatcga agaggtgatt taaattcatc cccggcggca agccggggag atttcattac
    10441 ggcagtggcg tcggtaacgg cgctttatcc agcgcggcag cacactctgc tgtcgggcga
    10501 tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg
    10561 tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt caacttaccc
    10621 ggcacggcaa tcacccgctg ccactgacgg caatccagag tatcgccctc tttagtgaca
    10681 atcaggctgc ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag
    10741 tagcccgcca gccagtccgg cgcgggggtg ttcacgacat tgttatagtt ttcgacttcg
    10801 gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat cattcgtcct
    10861 gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggtt
    10921 cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa ataaatttgc
    10981 atggtgtatg attcgcgact cttgtttcaa accttctgag ttcagaggct acattcatgt
    11041 catggcagca attcaaacac gcctggttga ttaaattctg ggcgcccatc cccgcggtca
    11101 tcgcggcggg tattctctcc acttactatt ttggcattac tggcaccttt tgggctgtca
    11161 cgggtgaatt tacccgttgg ggcggccagc tcctgcaact gttcggcgtc catgctgaag
    11221 agtggggtta ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggga
    11281 tgatgatcct cggtatgttt ggcggctgct ttgccgcagc gctgtgggcc aacaatgtca
    11341 aactgcgaat gccgcgcagc cgtatccgca ttatgcaggc catcattggc ggcattatcg
    11401 ccggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt accggtattc
    11461 ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt tcatggtttg
    11521 gcgcgcgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg
    11581 ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc
    11641 tggtcttttt cggcatgctg ggctgggcgc tgctcacggc gatgaaccaa cccaaactgg
    11701 ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caactctgct
    11761 ttacttcagc gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca
    11821 ttattggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac
    11881 ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg tttactgttt ggttttggca
    11941 tcgtgctggc aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc
    12001 actactggtg ggtcggtctg ggcaacgtga tcggctcaac gattctggcg tattactggg
    12061 atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg
    12121 gcccgatggg tggcctgctg gtgacatatt tgctgttgtt tactgcgctg atgttgatta
    12181 tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gactgctaag gagatcgcat
    12241 gaaaaatatc gttcctgatt accgccttga tatggtgggt gaaccctgcc cttatccggc
    12301 agtcgcaact cttgaggcga tgccgcagtt aaaaaaaggg gaaatcctgg aagtgatgag
    12361 cgactgtcca cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt
    12421 gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat
    12481 tcccgctgtg gcccacagcg ggaacatcgt tgcctctgac acatagattt tacccgcccc
    12541 ctctcctatt cttttcagta tgatctcagg aggtttctat gtgtggacgc tttgcccaat
    12601 cccaaacgcg tgaagattac cttgcgcttc tcgcggaaga tattgaacgc gatattccct
    12661 acgatcccga acccattggc agatacaacg tcgcgccggg aaccaaagtt cttctgctca
    12721 gtgaacgtga tgaacacctt catctggatc cggttttctg gggatatgct cccggatggt
    12781 gggataaacc gccgctgatt aacgcccgcg tagaaactgc ggccaccagt cgtatgttta
    12841 aaccgctctg gcaacatggt cgggcaatct gttttgccga tggctggttt gagtggaaaa
    12901 aagaaggcga caaaaaacag ccttatttta tctatcgcgc tgatggacaa cctgttttta
    12961 tggccgcgat aggtagcaca ccgtttgagc gtggtgacga agccgaagga tttttaattg
    13021 tcactgctgc agcagaccag ggtctggtag acattcatga ccgccgccca ctggtactgt
    13081 cgccagaagc tgcgcgggaa tggatgcggc aagagattgg cggtaaagaa gcatcagaaa
    13141 ttgctaccaa cggctgtgtg ccagcaaacc agttcacctg gcacccggta tcgcgcgcgg
    13201 taggtaatgt caaaaaccag ggcgcggagt taattcaacc tgttt
//
LOCUS       DAZGTR010000113        13091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGTR010000113 DAZGTR010000000
VERSION     DAZGTR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.115"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..1600
                     /gene="pitB"
                     /locus_tag="ACHLCY_003764"
                     /pseudo
     CDS             102..1600
                     /gene="pitB"
                     /locus_tag="ACHLCY_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(1649..2341)
                     /gene="yghT"
                     /locus_tag="ACHLCY_003765"
     CDS             complement(1649..2341)
                     /gene="yghT"
                     /locus_tag="ACHLCY_003765"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0370937.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AIMDFMTARGYH"
     gene            2515..3228
                     /gene="yghS"
                     /locus_tag="ACHLCY_003766"
     CDS             2515..3228
                     /gene="yghS"
                     /locus_tag="ACHLCY_003766"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0370938.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3260..4018
                     /locus_tag="ACHLCY_003767"
     CDS             3260..4018
                     /locus_tag="ACHLCY_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370939.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4064..5413
                     /locus_tag="ACHLCY_003768"
     CDS             4064..5413
                     /locus_tag="ACHLCY_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP0370940.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5413..6237
                     /locus_tag="ACHLCY_003769"
     CDS             5413..6237
                     /locus_tag="ACHLCY_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370941.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6249..6809
                     /locus_tag="ACHLCY_003770"
     CDS             6249..6809
                     /locus_tag="ACHLCY_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0370942.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6840..7910
                     /gene="lptF"
                     /locus_tag="ACHLCY_003771"
     CDS             6840..7910
                     /gene="lptF"
                     /locus_tag="ACHLCY_003771"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0370943.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7907..8986
                     /gene="lptG"
                     /locus_tag="ACHLCY_003772"
     CDS             7907..8986
                     /gene="lptG"
                     /locus_tag="ACHLCY_003772"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0370944.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9022..10194)
                     /locus_tag="ACHLCY_003773"
     CDS             complement(9022..10194)
                     /locus_tag="ACHLCY_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0370945.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEVIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10194..10442)
                     /locus_tag="ACHLCY_003774"
     CDS             complement(10194..10442)
                     /locus_tag="ACHLCY_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0370946.1"
                     /translation="MVNREIVMDYILFCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10474..11388)
                     /locus_tag="ACHLCY_003775"
     CDS             complement(10474..11388)
                     /locus_tag="ACHLCY_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0370947.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDSVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11385..13073)
                     /locus_tag="ACHLCY_003776"
     CDS             complement(11385..13073)
                     /locus_tag="ACHLCY_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP0370948.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3116 a         3249 c         3410 g         3316 t
ORIGIN      
        1 cactcttttt atcgttaaaa aatgatattt cactttgccc atgccgttaa agtcactgat
       61 aatgcgtccg ttcgtaaatt caaaatggcg taatctaata tatgctaaat ttatttgttg
      121 gccttgatat atacacaggg cttttgttat tgcttgctct ggcatttgtg ttgttctacg
      181 aagcaatcaa tggttttcat gacacggcga atgcggtggc aaccgttatt tatactcgtg
      241 ccatgcaacc acaacttgct gtggtgatgg cggcattttt taactttttt ggcgtgttat
      301 tgggcggact tagcgttgcc tatgccattg tccatatgtt accaaccgat ttgttgctga
      361 atatggggtc aacccacggt ctggcgatgg tcttttccat gctgctggcg gcgattatat
      421 ggaacctggg gacgtggttc ttcggtttac cggcctccag ttcgcacacc ttgattggcg
      481 cgattatcgg catcggttta accaacgcgc tgttaaccgg ctcatcggtg atggatgcgt
      541 taaacctgcg tgaagtgacc aaaattttct cctcgctgat tgtttcccct atcgtcggcc
      601 tggtcattgc gggtggtctg attttcctgc tgcgccgttt ctggagcggg acgaaaaagc
      661 gtgaccgtat tcaccgcatt ccggaagatc gcaaaaagaa aaaaggcaaa cgtaaaccgc
      721 cattctggac gcgtattgcg ctgattgttt ccgctgcggg cgtggcgttt tcgcacggcg
      781 cgaacgacgg gcagaaaggg atcggcctgg tgatgctggt actggtgggg attgcccctg
      841 ctggcttcgt cgtcaatatg aacgcgtccg gctatgaaat tacccgtact cgcgatgccg
      901 tctccaactt cgaacactat ctgcaacagc atcctgaact gccgcagaag ttgattgcga
      961 tggaacctcc attgcctgcg gcatcgactg atggcgcgca agtaacagag tttcactgtc
     1021 atccggcaaa tacctttgat gcgattgcgc gcgttaaaac gatgctgcca ggcaatatgg
     1081 aaagttacga gccgttaagc gtgagtcagc gcagccagct gcgccgcatt atgctgtgca
     1141 tctcagatac ctccgcgaag ctggcgaaac tgccaggcgt cagtaaagaa gaccagaacc
     1201 tgctgaaaaa actgcgcagc gatatgttaa gcaccattga gtacgctccg gtgtggatca
     1261 tcatggcggt agcactggcg ctcggcattg gcaccatgat tggctggcgt cgtgtagcga
     1321 tgaccatcgg tagaagattg gtaagcgcgg catgacgtat gcgcaaggca tggcggcaca
     1381 aatgacggcg gcagtgtcta tcggtcttgc cagttatatt gggatgcccg tctccacaac
     1441 acacgtcctc tcgtctgcag ttgcagggac gatggtggtg gacggcggtg ggttacagcg
     1501 taaaacggta accagcatcc tgatggcgtg ggtatttact ttaccggcgg cgatttttct
     1561 ttctggtggg ctgtactgga tagcattgca gttgatttaa ccttcctgaa aatgcccggt
     1621 cctggcgatc gggcatttcc atttttggct agtgataacc acgcgcggtc ataaaatcca
     1681 taatcgcttt ttctgcatca accaacacct gttccagtgg ctgattggca tcgatatcaa
     1741 ccagttgtgc accaccaaag gttaactgtg gcgttatggc tatctttctt gccagcgact
     1801 cccttttgtg gtcaggctta cgcgcgcagg caacttcaag gtcaacattg agtttgatga
     1861 ccagatcagg cttatggctc gccatccagt gaaacgcttt acgttcctgg cttgccagcc
     1921 atgagacaaa acgaccacct tcaacgttag gtgggaacac cgtaccatcg taagcgccag
     1981 gaatttggtc ctgaggataa cggtcggtta gaacaattaa cccgcgacga cgacaggcaa
     2041 gcatatgacg aaagcgcagt aagcgacggg cgacaaacgc tgtaattacc agcgccggaa
     2101 ctggtcctgg caattttttt gctgttttca cctgatttcg ttcaattgtt ttatgtaagg
     2161 attttcccat caacggtaat tttgtcactg cacgaccgac atttccggct tgttttccta
     2221 aatgaactct ttcggcagca ccatattttt cgacaacggt aataagatgt tcacacaccg
     2281 ttgacttgcc tgaaccatcg ctaccaataa cggcaattaa tggaggtgtt attgactgca
     2341 tgaatatatc cttaaattta aatatccatc caaaatattt atttggttaa tatgttttta
     2401 tgaaagcgta attcaggtca atgtcacaat taaccattgt cacaataagg ttgaacggat
     2461 attctgagca cggaccacgt aatattcaat acattattta ctgccgggtt tttcgtgaat
     2521 caacgtaata tgtcaataat taattccacg ccagtgcgtg ttattgccat tgtaggatgt
     2581 gatggttcag gtaaatcgac cctcacggca agcctggtaa atgaactggc agcaagaatg
     2641 ccaacagaac gcatttatct cgggcaatcg tccgggcgaa ttggcgaatg gatttcacag
     2701 ctccctgtta ttggcgcacc ttttgggcgt tatctgcgaa gtaaagcggc acatgtgcac
     2761 gaaaagccct caacaccgcc tggcaatatt actgcgctgg ttatctatct gctttcctgc
     2821 tggcgggcgt acaagtttcg caaaatgttg tgtaaaagcc agcaaggctt tctgctcatc
     2881 accgaccgct acccgcaggt tgaagtgccg ggttttcgct ttgatggccc gcaattggca
     2941 aaaaccacgg gcggtaacgg ttggataaaa atgttaaggc agcgcgagct gaagctgtac
     3001 caatggatgg catcttattt gcccgtattg ttgattcgtc ttggcattga tgaacaaacc
     3061 gcgtttgcgc gtaaacctga ccaccaactg gcagcgttac aggaaaaaat cgccgttacg
     3121 ccgcaactga cattcaatgg cgcaaagatc cttgaactgg atgggcgaca ccccgccgat
     3181 gaaattctgc aagcgtcact acgcgcaatt cacgccgcgc tttcctgatc accaaaatgg
     3241 ataggggatg atttaacgaa tggatgcact acaaactcaa actgttaata gtacaaccgc
     3301 gccgcagccc aactacattc cggggctgat tgcggtggtc gggtgtgatg gcaccggtaa
     3361 atccacactg accaccgacc tggtgaaatc gctgcaacaa cactggcaaa ccgagcggcg
     3421 ctatctgggg ctgctctccg gcgaagacgg cgacaaaatc aaacgattgc cgttggttgg
     3481 cgtctggctg gaacggcgac tggcggccaa atcctcgaaa acccaaagca tgaaaaccaa
     3541 atctccggcg ctatgggcgg cggtgattat gtactgcttc tcgctgcgaa gaatggcgaa
     3601 tctacgcaag gttcagcgac tggcgcaaag tggcgttctg gtggtcagcg atcgcttccc
     3661 gcaggctgaa atttcgggct tttattatga tggaccgggg attggcgtcg aacgtgcgac
     3721 cgggaaaatc agcatgtttc tggcgcagcg cgaacggcgt ttataccaac aaatggcgca
     3781 atatcgcccg gaattaatta ttcgcctggg cattgatatt gagactgcca tctcccgcaa
     3841 gcctgaccat gactatgccg agctgcagga caaaatcggc gtcatgtcga cgattggcta
     3901 taacggcaca aaaattcttg agattgattc cagagcgccc tacagcgaag tgctggaaca
     3961 ggcacagaag gcggtttctc tggttgccat cgtttctgac cgccgaagtt taacttaagg
     4021 ctggcgggat actttttact atcaacgata aggtcaggct gaattggcgg gttttaacat
     4081 caaacattgg tttgcagatg gcgcgtttcg caccattatt cgcaatagcg cctggttagg
     4141 ctccagtaat gtcgtgagcg ccttgctggg tctgttggcg ctctcgtgtg ccggtaaagg
     4201 gatgacgccc gccatgtttg gcgtactggt gattgtgcaa tcgtacgcca agtcgatcag
     4261 cgattttatt aagtttcaga catggcaact ggtggttcag tacggaacgc cagccttgac
     4321 caacaataat ccgcagcaat ttcgcaatgt cgtctcattt tccttctcgc tggatatcgt
     4381 cagcggcgcg gtggcgattg tcggcggcat tgccttactg ccattccttt cccattcatt
     4441 aggtctggat gaccaaagct tttggctggc ggcgctgtat tgcacgctca ttccttcaat
     4501 ggcttcctcc acgccgaccg gcattctgcg tgcggtagat cgcttcgatt taattgctgt
     4561 acagcaggcg acgaaacctt ttctgcgcgc agcggggagc gtcgtagcct ggtattttga
     4621 ctttggtttt gcgggttttg ttattgcctg gtacgtgtcg aatctggttg gcggcaccat
     4681 gtactggtgg tttgcagcgc gcgaattacg ccgcagaaat atccataatg ccttcaaatt
     4741 gaatctgttt gagtctgccc gacacatcaa aggcgcgtgg agttttgtct ggtcaaccaa
     4801 cattgcccac tccatctggt cggcgcgtaa ctcgtgcagc acagtgctgg tggggatcgt
     4861 gttaggaccc gctgccgccg ggttatttaa aatcgccatg acattcttcg acgccgccgg
     4921 aacgccagcg ggtttgctgg gtaaaagttt ttacccagag gtgatgcgtt tagatccgcg
     4981 caccaccaga ccgtggttgt taggtgtgaa gtccggttta ctggcgggcg gaatcggtat
     5041 tctggtggcg ctcgcggtgt ttatcgtcgg caagccgctc atttcgctgg tgtttggcgt
     5101 taagtatctc gaagcgtatg acctgattca ggtgatgctg ggcgcaattg tgatttcgat
     5161 gctgggcttc cctcaggagt cattgctatt gatggcggga aaacagcgcg cttttctcgt
     5221 ggcgcaaacc atcgcctcaa tcggctacat cgtattgctg ttcatgttct gtcatctgtt
     5281 tggtgtgctg ggagcagcgt ttgcttactt cggtgggcaa tgtctggacg tggcgctctc
     5341 gttaattcca accttaaagg cattttttca gcgccattcc ttgctttata acgcagccgg
     5401 agagaaatcc tgatgatgag aaaatatttc ccgcttgagg cgagcgagcg tctatttgtt
     5461 gcgattgaag aagatgatgt cgttgatgca caggttagtt tgccgccgac tattgcacta
     5521 agttgtacca ccgaaatcat tcatgataat tacgcgttat gcctgcaatt ctggctcaat
     5581 ggcgttgatc gtcaggaatt actgcgttta gttcgcaaac aagccaaagg cgatgagctg
     5641 actgctgatg agcgcaaaca atttaaatac atgcgagcgc gctacaagca cctgcgtttt
     5701 gcgcaacggc tgtatttaaa gaagcatcag gccggatttt tattcggcaa aacgaccgtt
     5761 tttctggggc gttttcagga cggctttcgt aacggtaaga aaaatattgt gtcgtattac
     5821 ggcaacctgt tacggattta tttaagctct ccggtctggt cgttggtgaa ctattcatta
     5881 cgccatagcc agttagaaag cgtaagcagt tttattgcct atcggcaaaa gcaaatgcat
     5941 accctgaaag agattattgc caaaccgcga ctaaccggca gggaatttca tgatgtgcga
     6001 aaaatcatca gccaacaggt ttcttattac gacaccttaa gatcgcttga tcctgaaaat
     6061 aaagaggcgt tacagatttc ccgtttcctc gccgccatta atggcctgat gggagataaa
     6121 cacgacgaca tggtggcgga tgatatggaa aaccgccagt cgtatgatgc accgttggcg
     6181 ctggatagcg atattcgtca gcgcttagag ctgctgattt cgcgttttcc gctatagccg
     6241 gagcaatgat gtcatctcgt ttgattattg cgttgattat catgctctta gccccgggtg
     6301 tgcaggcaca caatttcgtc actggcaaaa cggttacccc tgtttatatc caggaaggcg
     6361 gcgagctgct tctgaatagc gacgacgaaa ttcactacca aaagtggaac agcacacaat
     6421 tagcaggaaa agtccgcatt attcagtata tcgccgggcg taagtcggcg aagaaaaaga
     6481 actcactgtt aattaaagcg gttgaagcgg caaactttcc gcaggatcgt tttcagccca
     6541 ccaccattgt gaacaccgat gatgccatct ttggtactgg ctattttgtt gttggcaaaa
     6601 tcgaaaaaaa taaacgccgc tatccgtggg cgcaattcat tattgacggc aatggacagg
     6661 gacgcgttgc ctggcgcttg ccggagcaga gttcaaccat tctggtgctg aataaagccg
     6721 ggcaaattca gtgggcaaag gacggcagcc tgacgccgga agaggtagat catgtcattg
     6781 cgctggcgca gaaactgatc aatgaataaa aaattcaaag gattgagggc gttagtgaga
     6841 tgaagctggt tgaacactac atcatgcgtg gtacgcgccg tctggtgctg attatcgtcg
     6901 gttttctgat ctttattttc gccagctact ccgcacagcg ttatctgacc gaagcggcaa
     6961 atgggacatt agctcttgat gttgtgctgg atatcgtttt ctacaaagtg ctgattgcac
     7021 tggagatgtt gttacctgtt ggtctgtatg tgtcagttgg cgtgacgcta gggcagatgt
     7081 ataccgactc ggaaattacc gctatctctg cggcgggggg cagtccggga cgcttgtaca
     7141 aagccgttct ttatctggcg ataccgctaa gtatttttgt cacccttctg tcgatgtatg
     7201 gtcgtccgtg ggcttatacg cagatttatc aactggagca gcagtcgcag tcggagctgg
     7261 atgttcgcca gttgcgggca aagaaattta acactaacga taacggacga atgatccttt
     7321 cgcagacggt tgatcaggat aataatcgcc tgactgacgc gctgatttat acttctactg
     7381 ccaatcgaac ccgcattttc cgcgcccgtt cggttgatgt ggttgaccca tcacccgaga
     7441 aaccgaccgt tatgttgcac aacggcactg cctatcttct cgatcatcag gggcgtgacg
     7501 acaacgaaca gatctaccgt aatctgcaat tacatctgaa tccgctggat caaagcccta
     7561 acgtcaaacg caaagcaaaa tcggtcacgg agctggcgcg ctccgccttt cctgccgatc
     7621 atgccgaact gcaatggcga caaagccgtg gcctgacagc attgttgatg gcgctgctgg
     7681 ccatttcatt aagtcgggta aaaccgcggc aagggcgatt ttcaacatta ttgccactga
     7741 cgttgctgtt tattgccatt ttttatggcg gcgacgtctg ccgtacgctg gtggctaacg
     7801 gcgcgattcc cctcattcct ggtttgtggt tagtacccgg actcatgcta atgggcctgc
     7861 tgatgctggt cgcacgcgac ttctctttgc tgcagaaatt ttcccgatga atgttttcag
     7921 tcgctattta atccgtcatc tctttctcgg ttttgccgcc gccgcagggc tattgctgcc
     7981 gctttttacc accttcaacc tgattaacga actggatgat gtcagcccgg gcggttatcg
     8041 ctggactcag gcggtgctgg tggtgctaat gaccttaccg cgcacactgg tcgaactttc
     8101 gccgtttatc gcgttattgg gtggtattgt tggactgggg cagttatcga aaaacagtga
     8161 gcttaccgcc attcgcagca cggggttttc tatcttccgt attgcactgg tggcgctggt
     8221 tgccgggata ttgtggactg tttcgttagg cgcgatagat gagtgggtgg cgtcgccatt
     8281 gcagcaacag gcgttgcaaa tcaaatcgac cgccaccgcg ttgggggagg acgatgacat
     8341 taccggcaat atgctgtggg ccaggcgtgg taatgaattt gtgacggtga aatcgctgaa
     8401 cgagcagggc cagccggtgg gcgtggagat atttcattat cgcgacgatc tctcgcttga
     8461 atcctacatt tatgcacgca gtgccaccat taaagacgac aaaacgtggg tcctgcatgg
     8521 tgtgaatcat aaaaaatggc tgaatggcaa agaaacgctg gaaacattag ataatcttgc
     8581 ctggcaatcg gccttcacca gtatgaatct tgaagagtta tcaatgccgg ggaatacttt
     8641 ttctgtccgt cagcttaatc attacatcca ttatttgcag gaaaccgggc aacccagcag
     8701 cgaataccgc cttgcactgt gggaaaaact ggggcaaccc atcctgaccc tggcgatgat
     8761 tttgctggct gtgccgttta cctttactgc cccgcgttcg cctgggatgg gtagccgtct
     8821 cgctgtaggc gtcatcgtgg gcttactcac ctggatcagc tatcaaatca tggttaatct
     8881 gggattgcta tttgcgttaa gcgcacctgt taccgcgctc ggtttaccgg tagcgtttgt
     8941 gttggtggcg ttgagcctgg tgtattggta tgacagacaa cattaaaccc gccgtggcgc
     9001 aaacaccacg gcgggctcac attactgctt cagagtagca aatgcctgaa tgatggcatc
     9061 gatttgctca cgactgtggg cagcatttac gctacagcgc agcagcgtga tgcctgcagg
     9121 cgcggcaggc ggcaggataa gattcacata cacgcccagc gagataagct cgcgccagat
     9181 gcgtaaacct tcctctttcg aaccgattat tactggcact accgggctaa tatgtgaact
     9241 taactcataa cccagttttt gcagaccgtc atacagatgg ttggcgttat ccatcagctt
     9301 ttgccgcagc tctggttgtg tggcaatagt tcttaacgac gagcgcacgg tcgcaatgca
     9361 ggacggagaa ggagaggcgg taaaaatata cgggcggctg ccgtagcgca gcacttccat
     9421 cgcttccgaa ccgacagcaa aaccgccaat cgaggccaga cttttactga aagtaccaac
     9481 gataatatcc acatcatctt caacgccgac agcttcagcc agtccgcgcc ccgttgcgcc
     9541 taacacgcca aacgaatgcg cttcatcgac aatcaaataa ccaccgagac gacgtttgat
     9601 atcaacaatt tccgccagcg gcgcaacgtc accgagcatg ctgtagatgc cctcaacgat
     9661 gataatcacc tctttcgccc gttcaccgag gcgaaccatg cgccgttcca gatctttagc
     9721 atcgttatgg cggaaacgaa taatttccgc gccaccgaga gaacaggcgt cataaatact
     9781 ggcgtggctg tcggcatcca gtaacaccac ggcgttatgg tcggcaagtg cgctgatgac
     9841 gcctaaatta gcggtataac cggttgaaaa aacgatagcg gtagggcgat tgaaaaactc
     9901 ggcaatctct ttctctaacg ccaggtgcgg cgcataactg ccgtttgcca tacgagaacc
     9961 ggtggtgccg gtgccttggg ccgctaacgc tgcctgacct tcgctgatgg catcatgatt
    10021 aaaggttaaa ccgagataat tattggtccc ggccagaatc acttttttat tgccgatccg
    10081 gccttcggtg gcggaataca cttcatcaat acaggtgcca aacggtgtta acccgttgtc
    10141 actaaattgc agtcgctcgc cagcaaggcg tgcatattta tcgtatagcc ccattattta
    10201 ttctccagcc atggaagtag agtttccatt aattgcgcag gggttttgac atccagcaga
    10261 atattaatag gaatggaaat atcgaatctg tcttccagca tcattaagag atccatcact
    10321 ttgatcgact ccaggccaag atcgttgacg agatcgctgt cgggcttaat ttccacaccg
    10381 ttttcgacca aatcctgtaa acagaaaagg atgtaatcca ttactatttc acgatttacc
    10441 atagaaacgt taacacacct ttaaacaaga caatcaaaat agcccgttgc gtaatgcgtg
    10501 ttccaggcta atcccgggaa accaattaat atcttcagaa atacggttat tacttgccga
    10561 ccagtcggcg tgggttaatt cacgaatttt cgagcgggtc agcataggtt ctttacctgc
    10621 caggcgactc aacgcggtac tgatatccgc aaggcaggtg agtaccggca gaggaatacc
    10681 aaccattcgc acggaaccgc aacggacgtt ggcggcaagt tgctgtatgc gttgccagtc
    10741 atagccgcca gcgacgctat cgcataattc ataggtttgc gtctgtacag tttcggcgct
    10801 taaccactga cccactgctt gcgcaaaatc ggtgacgtgc aggaaagaga gctgtgtatc
    10861 tggtgcacca agtcgtggca gcaggccgcg cagcatccag tcaaacagcg gttttaactc
    10921 tttatcgccg ggaccataca cggctgtcgg gcgaaaaacg cccagcgtaa tttcgtcagc
    10981 cattgcagtc agccgttgtt cggcgatgtg tttggaattt gcgtaccagg agagctcggg
    11041 atggcgcgcc gccagcgaag agataaacag aaaacgttgg caaaagcagc tctcttttgc
    11101 tgcttgcatc aggcgcaggc tgccgtcaac gttacagcgg gtgaaaatct cttctttgtg
    11161 cccgcgcact tgcccggcgc aatggaccac tgcgctggct ccggcaacca gcttgctaag
    11221 tgaatgagta tcttccagcg aaccgcgcac ccaggtgaga ttatcattga cgtgagcgcg
    11281 ggcagtacgc gtcaatgcgc gaacgtgaaa gccgcgggcg agcaggttat cgataatata
    11341 tttaccgata aacccggtag cgcccgtcac cgcgacagtt tgattcatgc cagggattcc
    11401 tgcacattaa gactggcagc ataagccttc tgataacgtt ttttcgcttc cgcacgggca
    11461 ggcttgccgg aggacgttcg gggaatactg tggggcggca acagatcgat agccgcggtc
    11521 acgccaaatt cgctttggat ccgtgccgcc agcgcgtgga taagctgccc gcgacgttct
    11581 tcgtcgctga tccgacactg gatctgcaaa atgatttttt cctgggcggt aacaaaagca
    11641 atcgcatcgc cagaatgaat ttccggttct tgttccgcaa tatattcaat atcctgcggc
    11701 cagatattac ggccacgaat aataatcaga tctttaatgc gtccggtgac atacagataa
    11761 ccgtccagca gataaccgag gtcgccggtg tctaaccagc ccgtcgcggc aatctcgtct
    11821 tgcgaaacct ggtcgccaaa gtaaccgctc atcaggctgg ggccggagat gcaaatatgg
    11881 cctaccacac gttccgcgac cggcatacct gcttcattgc ggatttcaat gccatgttcc
    11941 ggcaacgctt tgccgcagtt gacgaaagtc gatacggcgc gtgtctctgc acccggcgcg
    12001 acggctttac cctgatattc gaggatgtcg cgatccactt cgttaaccac aaccccggag
    12061 gcttcatcag agaagctgac agccagcgca ttttctgcca gtccgtagca cggcatgaaa
    12121 gttttattgt caaagttaac ctgacggaaa cattcagcga attgatggag ttgttctgcg
    12181 gagatgggtt ctgcaccaat accagcgacg cgccagcagg aaagatccag ttcagcgaga
    12241 tctttttcat tcacgcggcg ctggcacaat tcatagccaa acggcggcgc aacggaaacg
    12301 gtgccgcgat ttttactgat caatttaagc cattgcagag gacgcatggc aaaatcctga
    12361 gtgcgcaaat aatctactga aagctgcgtg gcgacggggg tcaggagaaa gccgaccagt
    12421 cccatatcat ggtagaaagg cagccaggag acgcagcggt cgccagggcg taatttaatg
    12481 ccgtcgtggc ttatagcacg tagattagcc atcacttcgc gatgggtgat aatgacgcca
    12541 cggggaaaac gggtgctgcc ggaggtgtac tggaggtagg cgatatcgtt cggaactgga
    12601 cgctggagcg caacatcggc ttccggtaat gccttaaacc aggcgtggct taaaacatgt
    12661 aattcggggt tgttatgcgt cgcggcattg accagtggca accactcatc accagtgata
    12721 atggctgcgg gctggcaact tgccagtaaa ccctggagtt tggcgctcca ggaatcccgc
    12781 tgaccgacgc ccattggaat cgccaacggg acggcgacta agccagcata ctgacaggca
    12841 aaaaaagcct ctacaaaccc gctactcgtt tcggcaatca gcgccacgcg ttcgcctttt
    12901 ttcaggttca gcgataacaa ccgcttcgca ccagcttcgg cacgcgtttt taatgtctga
    12961 tattccagtt gatcttcaag ttggcaacgt ctgtcataaa aattcattcc ggcgctactc
    13021 agagcggcgt agtccaaagc atcaaccagc gttggaaaat cggcatagcg cataggtagg
    13081 gaatgcgtaa a
//
LOCUS       DAZGTR010000114        13077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGTR010000114 DAZGTR010000000
VERSION     DAZGTR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.116"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            171..884
                     /gene="aphA"
                     /locus_tag="ACHLCY_003777"
     CDS             171..884
                     /gene="aphA"
                     /locus_tag="ACHLCY_003777"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP0370949.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            995..1411
                     /locus_tag="ACHLCY_003778"
     CDS             995..1411
                     /locus_tag="ACHLCY_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP0370950.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1415..1771
                     /locus_tag="ACHLCY_003779"
     CDS             1415..1771
                     /locus_tag="ACHLCY_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0370951.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(1806..4628)
                     /gene="uvrA"
                     /locus_tag="ACHLCY_003780"
     CDS             complement(1806..4628)
                     /gene="uvrA"
                     /locus_tag="ACHLCY_003780"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP0370952.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            4882..5418
                     /gene="ssb1"
                     /locus_tag="ACHLCY_003781"
     CDS             4882..5418
                     /gene="ssb1"
                     /locus_tag="ACHLCY_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP0370953.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5517..5798)
                     /locus_tag="ACHLCY_003782"
     CDS             complement(5517..5798)
                     /locus_tag="ACHLCY_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP0370954.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIELVMIEEMNQTPPQWPMILT"
     gene            6228..7814
                     /gene="pdeC"
                     /locus_tag="ACHLCY_003783"
     CDS             6228..7814
                     /gene="pdeC"
                     /locus_tag="ACHLCY_003783"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP0370955.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(7817..8140)
                     /gene="soxS"
                     /locus_tag="ACHLCY_003784"
     CDS             complement(7817..8140)
                     /gene="soxS"
                     /locus_tag="ACHLCY_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP0370956.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8226..8690
                     /gene="soxR"
                     /locus_tag="ACHLCY_003785"
     CDS             8226..8690
                     /gene="soxR"
                     /locus_tag="ACHLCY_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP0370957.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9236..10585
                     /gene="ghxP"
                     /locus_tag="ACHLCY_003786"
                     /gene_synonym="yjcD"
     CDS             9236..10585
                     /gene="ghxP"
                     /locus_tag="ACHLCY_003786"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP0370958.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10737..12386
                     /locus_tag="ACHLCY_003787"
     CDS             10737..12386
                     /locus_tag="ACHLCY_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP0370959.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12540..>13077)
                     /locus_tag="ACHLCY_003788"
     CDS             complement(12540..>13077)
                     /locus_tag="ACHLCY_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0370960.1"
                     /translation="YMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNK
                     AILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNFNNANLSNCHFNCSILTKACM
                     FNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTED
                     NINAVAMKITADIMQHAD"
BASE COUNT         3057 a         3202 c         3436 g         3382 t
ORIGIN      
        1 caaaaaaaga caggaacgta tttactgggt taaatataat catcctgctt ttcatcacaa
       61 aaaccgcaga taatccttcc tttccccggc agctggcgtt atggtcagat ggtttttgca
      121 acaaatctca caataaaaag tttcaacata ctgactattt agggaaaaat atgcgcaaga
      181 tcacacaggc aatcagtgcc gtttgcttat tgttcgctct aaacagttcc gctgttgccc
      241 tggcctcatc tccttcaccg cttaaccctg ggactaacgt tgccaggctt gctgaacagg
      301 cacccattca ttgggtttcg gtcgcacaaa ttgaaaatag cctcgcaggg cgtccgccaa
      361 tggcggtggg gtttgatatc gatgacacgg tacttttttc cagtccgggc ttctggcgcg
      421 gcaaaaaaac cttctcgcca gaaagcgaag attatctgaa aaatcctgtg ttctgggaaa
      481 aaatgaacaa tggctgggat gaattcagca ttccaaaaga ggtcgctcgc cagctgattg
      541 atatgcatgt acgccgcggt gacgcgatct tctttgtgac tggtcgtagt ccgacgaaaa
      601 cagaaacggt ttcaaaaacg ctggcggata attttcatat tcctgccacc aacatgaatc
      661 cggtgatctt tgcgggcgat aaaccagggc aaaatacaaa atcgcaatgg ctgcaggata
      721 aaaatatccg aattttttat ggcgattctg ataatgatat taccgccgca cgcgatgtcg
      781 gcgctcgtgg tatccgcatt ctgcgcgcct ccaactctac ctacaaaccc ttgccacaag
      841 cgggtgcgtt tggtgaagag gtgatcgtca attcagaata ctgacagagc gggagagcgt
      901 gacgctctcc cgaatgctgt ttttttaatc acacctttat cctttcgctg tcttgctgca
      961 aactgattaa gagagtttta tcaaggagca gcacatgtgg tatcaaaaga cgctcacgct
     1021 tagcgccaaa tctcgtgggt ttcatctggt aacggatgaa attctgaatc agctggctga
     1081 tatgccgcgc gttaacatcg gcttactgca tctgttgctg caacatacct ccgcctctct
     1141 gacacttaat gagaactgcg atcccaccgt acgccacgac atggagcgtt ttttcctccg
     1201 caccgttccc gacaacggaa attatgagca tgactatgag ggagcagacg atatgccttc
     1261 tcatatcaaa tcctcaatgc tgggaacatc gcttgtattg ccggtacata aagggcgtat
     1321 tcagaccggc acctggcaag gcatctggct gggggaacat cgcatccacg gcggatcgcg
     1381 tcgcatcatc gcgacactac aaggggagta aaaaatgacc atttcggagt tgctacaata
     1441 ttgcatggca aaaccaggcg cagaacagag cgtgcataac gactggaaag cgacgcagat
     1501 caaagtggaa gatgtactgt ttgcgatggt gaaagaagta gaaaatcgcc cagctgtttc
     1561 gctgaaaacc agcccggagc tggcggagct gctacgtcag cagcacagcg atgtgcgtcc
     1621 tagccgccat ctgaataaag cgcactggag caccgtgtat ctcgacggtt cgctgccgga
     1681 ttcgcaaatc tattatctgg tggatgcgtc ttatcagcag gcagtgaatt tactaccgga
     1741 agaaaagcgt aaattgctgg tacaactctg aaaggaaaag gccgctcaga aagcggcctt
     1801 aacgattaca gcatcggctt gaggaagcgt gccgtatgcg aagcttcgca ctccgcgacg
     1861 gtttctggcg taccggagac gaggatttcg ccgccgccac tgccgccttc tggtcccagg
     1921 tcgacaatcc agtcagcggt tttgatcacg tcgagattgt gctcaatcac cacaatggtg
     1981 ttgccctgat cgcgcagttt atgcagtacg tcgagcagtt gctgaatatc ggcgaagtgc
     2041 agaccggtgg tcggctcgtc gagaatatac agcgtctgcc cggtgccgcg ttttgacagc
     2101 tcacgcgcca gcttcacgcg ctgggcttca ccaccagaaa gtgtggttgc ggactgcccc
     2161 aggcgaatgt acgtcaggcc aacgtccatc aacgtttgca gcttacgcgc cagtgcaggt
     2221 acggcatcaa agaactcacg cgcctcttcg atggtcatat ccagcacttc gtggatggtt
     2281 ttgcctttgt acttaatttc cagcgtttca cggttatagc gtttaccttt acactgatcg
     2341 cacggcacgt agatatccgg caggaagtgc atctctactt tgatcacacc gtcgccctga
     2401 caggcttcgc agcgcccgcc acggacgtta aagctgaaac gtcctggcgt ataaccacgc
     2461 gcacgggatt ccggtacgcc cgcaaacagt tcgcgcacag gcgtaaacac gccggtatag
     2521 gtcgccgggt tagaacgtgg agtacgacca attgggcttt ggtcgatatc gatcacttta
     2581 tcgaaatgct ccagcccctg aatatcgcga tacggtgctg gttcggcgat ggtcgcccca
     2641 ttcaactggc gttgggcaat cgggaacagt gtgtcgttaa tcagcgtcga tttaccggaa
     2701 cctgaaaccc cggtgatgca ggtaaacaga cccaccggca gcgtcagcgt cacgtccttc
     2761 aggttgttgc cgcgtgcgcc tgtcagcttc agcacttttt ccggattcgc cggaacgcgt
     2821 ttcttcggca cttcaatctt gcgtttgccg ctcatgtact gcccggtcaa cgactccggc
     2881 accgccataa tcgcttccag cggaccttct gcgaccactt caccgccgtg aacacctgcg
     2941 cccgggccaa tgtcgatcac atggtcagcg gcgcgaattg cgtcttcgtc gtgctccacc
     3001 acaatcacgg tattaccgag atcgcgcaga tggataagcg tacccaacag gcgctcgtta
     3061 tcgcgctggt gcaggccgat agatggctcg tccagcacgt acataacgcc aaccaggccc
     3121 gcaccaatct ggctcgccag acggatacgc tgggcttcac cgccggaaag cgtttctgcc
     3181 gagcgggaaa gcgtcaggta attcaggccg acgttaacga ggaatttcaa acgatcgccg
     3241 atctctttaa ggattttttc cgcaatcttc gcccgttgac cagcgagttt gagattgttg
     3301 aagaattcca tcgcatgacc gatgctcatg tcggagatag caggcagcgg cgtattctcg
     3361 acataaacgt ggcgcgcttc ccgacgcaga cgcgtccctt cgcagctggc gcatgggcga
     3421 ttgctgataa acttggctaa ttcttcacgt accgcactgg attctgtctc tttataacgg
     3481 cgctccatat tgtgcagcac gccttcgaac ggatgacgac ggatggaggt atcgccacga
     3541 tcgttcatgt atttgaattc aatgttttct ttgccagaac cgtacaacac cactttatgc
     3601 acgttcgcgc tcaggctgcc ccacggtgct tcgacgtcga acttatagtg atctgccagc
     3661 gatttcagca tctggaagta atagaagttg cggcgatccc agccacggat cgcaccgcca
     3721 gccagtgaca gctcggggtt ttggatcacg cggtcaggat cgaaatattg ctgtacgcca
     3781 aggccgtcac aggtcgggca ggcacctgcc gggttgttaa acgaaaacag tcgcggctcc
     3841 agttcacgca tactgtagcc gcaaattggg caggcgaagt tggcggagaa cagcagctct
     3901 tccgctttcg ggtcgtccat atccgccact accgctgtac caccggaaag ctccagcgcg
     3961 gtttcaaacg actccgcaag acgttgggta agatcgtcac gcaccttgaa gcgatcaacc
     4021 accacttcaa tggtatgttt cttttgcagt tccagtttcg gcggatcgga aagatcgcag
     4081 acttcgccat caatacgagc acggatgtaa ccctggcttg ccaggttctc cagcgttttg
     4141 gtgtgttcgc ctttgcgctc tttaatgatt ggcgcgagca gcatcagacg cttgccttcc
     4201 ggctgcgaca gcacgttatc caccatctgg ctgacggttt gcgccgccag cgggacgtcg
     4261 tggtccggac agcgcggttc gccgacgcgg gcgaacaaca aacgcaaata gtcgtggatt
     4321 tcggtgattg tccccaccgt agaacgcggg ttatgagacg tcgatttctg ctcaattgag
     4381 atggcaggag aaagcccctc aatatgatcg acgtccggct tttccatcag tgacagaaac
     4441 tgccgcgcgt aggcggaaag ggattcaacg taacggcgct gcccttcggc atataaggtg
     4501 tcgaaagcga gcgaggattt gccagaaccc gaaagcccgg tcacgacaat gagcttgtcg
     4561 cgggggataa cgaggttgat gtttttgaga ttatgggtgc gggcgccccg aacttcgatc
     4621 ttatccattc acctttcccg gattaaacgc ttttttgccc ggtggcatgg tgctaccggc
     4681 gatcacaaac ggttaattat gacacaaatt gacctgaatg aatatacagt attggaatgc
     4741 attacccgga gtgttgtgta acaatgtctg gccaggtttg tttcccggaa ccgaggtcac
     4801 aacatagtaa aagcgctatt ggtaatggta caatcgcgcg tttacactta ttcagaacga
     4861 tttttttcag gagacacgaa catggccagc agaggcgtaa acaaggttat tctcgttggt
     4921 aatctgggtc aggacccgga agtacgctac atgccaaatg gtggcgcagt tgccaacatt
     4981 acgctggcta cttccgaatc ctggcgtgat aaagcgaccg gcgagatgaa agaacagact
     5041 gaatggcacc gcgttgtgct gttcggcaaa ctggcagaag tggcgagcga atatctgcgt
     5101 aaaggttctc aggtttatat cgaaggtcag ctgcgtaccc gtaaatggac cgatcaatcc
     5161 ggtcaggatc gctacaccac agaagtcgtg gtgaacgttg gcggcaccat gcagatgctg
     5221 ggtggtcgtc agggtggtgg cgctccggca ggtggcaata tcggtggtgg tcagccgcag
     5281 ggcggttggg gtcagccaca gcagccgcag ggtggcaatc agttcagcgg cggcgcgcag
     5341 tctcgcccgc agcagtccgc tccggcagcg ccgtctaacg agccgccgat ggactttgat
     5401 gatgacattc cgttctgatt tgtcattaaa acaataggtt atattgtttt aaggtggatg
     5461 attaaagcat ctgccagcca taaaaaagaa gcctccgtta tggaggcttc tacgtatcag
     5521 gtcaaaatca ttggccattg tggcggtgtc tgattcatct cttctatcat caccaactcg
     5581 atgttgtgcc agatagcggt gatgtccgcc attgtgatgc caagcaaccc cattgcgaac
     5641 caacaggcgg aaacaacgcc cagaccgctg ctgatcaccg caagcccgac ataatcagac
     5701 cgacgaaaac ggatgttgag tgtgctggcc agaaacatca ttacggcact aagaagttgc
     5761 cagcgaagaa gaaccacgcc agtggtgagg gtagccatca aacaattcct ctgaaaagag
     5821 ccgatgccct ggacagcgcg gcgttgttca cgggaggcgt ggtacactct ggctatcgcg
     5881 gggcttgcag aacacaaaaa tgaaacactc tgtttgtttc attaattttg tgaactatat
     5941 cacaattgat tgtttgttag ccatattagg ccgtgacttt tattgctgta cagattatgt
     6001 ggtttttcag tggcattaag ggcatatctt cccgccgcct ctgcattcct gtaggaaatt
     6061 aattttgaat atcaatgaat tattttcatc caggtgacga ttagaaaggt atcaatttca
     6121 aatcaggcaa aagtgctatt tataccgtaa gatttatcta aagacgtcgg tacccagggt
     6181 tttcaccttg caatggccgg gtataaacag gcaggaaatt gatagcaatg agtcatcgtg
     6241 cacgacacca attactggcg ttgccgggca ttatcttttt agttctcttt cccatcattc
     6301 tttcgctatg gattgccttc ctttgggcaa aatcagaagt gaataatcag ctccgaacct
     6361 ttgctcaact ggcactggat aaatccgagc tggtcattcg ccaggcagat ttagtgagcg
     6421 atgcagctga acgctatcag gggcaagttt gcactccagc ccatcaaaag cgaatgttga
     6481 atattattcg tggctatctt tatattaatg aattgatcta tgcccgtgat aaccattttt
     6541 tatgctcatc gctgatagcg cctgtaaacg gctatacgat tgcaccggcc gattataagc
     6601 gtgaacctaa cgtttctatc tattattacc gcgatacgcc ttttttctct ggctataaaa
     6661 tgacctatat gcagcgggga aattatgtgg cggttatcaa ccctctcttc tggagtgaag
     6721 tgatgtctga tgatccgaca ttgcaatggg gtgtgtatga tacggtgacg aaaacctttt
     6781 tctcgttaag caaagaggcc tcggcagcaa cgttttctcc gctgattcat ttgaaggatt
     6841 taaccgtaca aagaaatggc tatttatatg cgacagttta ttcgacaaaa cgcccaattg
     6901 cagccattgt tgcgacttca tatcaacgtc ttataaccca tttttataat catcttattt
     6961 ttgcgttgcc cgccggtatt ttggggagtc ttgttctgct attactctgg ctacgtattc
     7021 gacaaaacta tttatctccc aaacgtaaat tgcaacgcgc cctcgaaaaa catcaacttt
     7081 gtctttatta ccagccaata atcgatatca aaacagaaaa atgtatcggc gctgaagcgt
     7141 tgttacgttg gcttggtgag caggggcaaa taatgaatcc ggcagagttt attccgctgg
     7201 cagaaaagga ggggatgata gaacagataa ctgattatgt tattgataat gtcttccgtg
     7261 atctgggcgc gtacttggca acacatgccg atcgctatgt ttctattaac ctgtcggcct
     7321 ccgattttca tacgtcacgg ttgatagcgc gaatcaatca gaaaacagag caatacgcgg
     7381 tgcgtccgca gcaaattaaa tttgaagtga ctgaacatgc atttcttgat gttgacaaaa
     7441 tgacgccgat tattctggct ttccgccagg ctggttacga agtggcaatt gatgattttg
     7501 gtattggtta ctctaacttg cataacctta aatcattgaa tgtcgatatt ttgaaaatcg
     7561 ataaatcgtt tgttgaaacg ctgaccaccc acaaaaccag tcatttgatt gcggaacaca
     7621 tcatcgagct ggcgcacagc ctggggttaa aaacgatcgc tgaaggcgtc gaaactgagg
     7681 agcaggttaa ctggctgcgc aaacgcggcg tgcgctattg ccagggatgg ttttttgcga
     7741 aggcgatgcc gccgcaggtg tttatgcaat ggatggagca attacccgcg cgggagttaa
     7801 cgcgcgggca ataaaactac aggcggtggc gataatcgct gggagtgcga tcaaactgcc
     7861 gacggaaaac gcgggagaag gtctgctgcg agacataacc caggtccatt gcgatatcaa
     7921 aaatcggacg ctcggtggtg cgcaactcaa cggcggccag taacaggcga cgttggcgaa
     7981 tgtaatcgcc aagcgtctga tgcgtcaccg tgcggaacat tcgttgcaag taccactttg
     8041 aatagcctga tttttttgcg actacatcaa tgttaagcgg ctggtcaata tgctcgtcaa
     8101 tccatgcgat aagatcctga ataattttct gatgggacat aaatctgcct cttttcagtg
     8161 ttcagttcgt taattcatct gttggggagt ataattcctc aagttaactt gaggtaaagc
     8221 gatttatgga aaagaaatta ccccgcatta aagcgctgct aacccccggc gaagtggcga
     8281 aacgcagcgg tgtggcggta tcggcgctgc atttctatga aagtaaaggg ttgattacca
     8341 gtatccgtaa cagcggcaat cagcggcgat ataaacgtga tgtgttgcga tatgttgcaa
     8401 ttatcaaaat tgctcagcgt attggcattc cgctggcgac cattggtgaa gcgtttggcg
     8461 tgttgcccga agggcatacg ttaagtgcga aagagtggaa acagctttcg tcccaatggc
     8521 gagaagagtt ggatcggcgc attcatacct tagtagcgct gcgtgacgaa ctggacggat
     8581 gtattggttg tggctgcctt tcgcgcagtg attgcccgtt gcgtaacccg ggcgaccgct
     8641 taggagaaga aggtaccggc gcacgcttgc tggaagatga acaaaactaa agcgccacaa
     8701 gggcgcttta gtttgttttc cggtctttgt ctttctctct atcccgctgg tacacaggag
     8761 ggtttccccc gacgtcaaca caccccattc gagcacgtgg tggaggttcc ggttggtgtt
     8821 gatgctttaa ttgtatgtca ccggcatttc ttcgccagtg taaaagtata ctttttatcc
     8881 gcaatatttt tgtcatctca gacgattttt tatcgcaatc ctgaacggta tacggctcga
     8941 taacgctgca atcttgcgca ccgacgataa cgtttgcgcg tcaattgcct ggtttttcat
     9001 cgtcaagaca ataaaagaga aaaaagcagc aaacttcggt tgaaaaagcc gctatgatcg
     9061 ccggataatc gtttgctttt tttaccaccc gttttgtatg cgcggagcta aacgtttgct
     9121 tttttgcgac gcagtaaatt gtcgcaaacc tggagcagga agataacgtt tcgctggcag
     9181 gggattgtcc gccacgcatc ttgacgaaaa ttaaactctc aggggatgtt ttcttatgtc
     9241 tacgccatca gcgcgtaccg gcggttcact cgacgcctgg tttaaaattt cacaacgtgg
     9301 aagcactgtc cgtcaggaag tggttgccgg gttaacaacg tttctggcga tggtctactc
     9361 ggtcatcgtc gttccaggta tgttgggtaa agcaggcttc ccgcctgcgg cagttttcgt
     9421 tgcaacctgt ctggttgccg gactcggttc tatcgtgatg ggtctgtggg ctaatctgcc
     9481 gttggcgatt ggttgcgcca tctccctgac ggcgtttacc gcattcagcc tggtgctggg
     9541 gcaacatatt agcgtacctg tcgcgctggg tgccgtgttc ctgatggggg tgctgtttac
     9601 ggtaatttct gccacgggta tccgtagctg gattttgcgc aacttgccgc acggtgtggc
     9661 gcacggcacg gggatcggta tcggtctgtt cctgctgctc attgccgcta atggtgtcgg
     9721 tctggtgatt aaaaacccgc ttgatggtct gcccgttgcg ctgggtgatt tcgcgacctt
     9781 cccggtgatt atgtcactgg taggtctggc ggtgatcatc ggcctggaaa aactgaaagt
     9841 ccctggtggc attctgctga ccattatcgg tatctcaatt gtcggtttga tcttcgatcc
     9901 taacgtccat ttctccggcg ttttcgccat gccttcattg agcgatgaaa acggcaattc
     9961 actgattggc agcctggaca ttatgggcgc gctgaatcct gtagtcctgc caagcgttct
    10021 ggcgctggtg atgacggcag tatttgatgc caccggaact atccgtgccg tcgccggcca
    10081 ggcgaacctg ctggataaag atgggcagat catcgacggt gggaaagcac tgaccactga
    10141 ctccatgagc agcgttttct ctggcctggt gggtgctgct ccggcagcgg tatacatcga
    10201 gtctgcggcg ggtacggcgg cgggcggtaa aaccggtttg acggctatca ccgttggcgt
    10261 gctgttcctg ctgattctgt tcctctctcc gctctcttac ctcgttccgg ggtatgcaac
    10321 ggctccggcg ctgatgtacg ttggcctgct gatgctgagc aacgtggcga aaatcgactt
    10381 tgctgatttt gttgatgcga tggcgggtct ggttacggcg gtattcatcg tgctgacctg
    10441 taacatcgta acaggcatca tgatcggctt cgcgactctg gtgattggtc gtctggtttc
    10501 cggcgaatgg cgcaagttga acatcggtac ggtcgttatc gccgtggcgc tggtgacctt
    10561 ctatgcgggt ggctgggcta tctaatcttt catcggattc tgaaaacggg tggcaatggc
    10621 tgcccgtttt tattttctcc gcacattgtg tgagcttttg cgatattctg aaaaaatgag
    10681 aattcaggca taacgtcttt ctcagatcat aaaaaaacat cgcaaacagg gaacgcatgg
    10741 aaatcttctt caccatactg ataatgaccc tcgtggtctc gctgtccggg gtggtcactc
    10801 gtgtcatgcc ctttcagatc ccgcttccgc ttatgcaaat cgccatcggt gcgctactgg
    10861 cgtggccgac gtttggtttg catgtggagt ttgatcctga actcttttta gtcttgttta
    10921 tcccgccgtt gctgttcgct gatggctgga aaacgccgac ccgtgaattt ctcgaacatg
    10981 gtcgagagat ttttggcctc gcgctggcgc tggtggtggt caccgtggtc ggcattggct
    11041 tccttattta ctgggtggtg ccgggcattc cgctgatccc cgcctttgcg ctggcggcag
    11101 tgctttctcc gaccgatgct gtggcgctct ccgggattgt tggcgaaggg cgcatcccga
    11161 aaaaaatcat gggtattttg cagggcgaag cgttgatgaa tgacgcctcc ggcctggtgt
    11221 cgttgaagtt tgccgtggca gtggcgatgg ggacgatgat cttcaccgtt ggcggtgcga
    11281 cggtcgaatt tatgaaagta gccattggcg gtattctcgc cggctttgtg gtgagctggc
    11341 tgtatggtcg ctcgctgcga ttcctcagcc gctggggcgg tgatgaaccc gcgacgcaga
    11401 ttgttctgct gttcttgctg ccattcgctt cttatctgat tgccgaacat attggcgttt
    11461 ccggcatcct cgctgcggtt gccgccggga tgaccatcac ccgctccggt gtgatgcgcc
    11521 gtgcgccgct ggcaatgcgc ctgcgcgcaa acagcacctg ggcgatgctg gaatttgtct
    11581 ttaacggcat ggtgttcctg ctgttaggtc tgcagctgcc gggtattctg gagacgtcgc
    11641 tgatggcggc agaaatcgac cctaacgtcg aaatctggat gctgtttacc gatattattc
    11701 tgatatatgc ggcgctgatg ctggtccgtt tcggctggct gtggacgatg aaaaagttca
    11761 gcaaccgctt cctgaagaag aagccgatgg agtttggttc gtggaccaca cgagaaatcc
    11821 tgatcgcgtc tttcgccggg gtgcgtgggg cgatcacgct ggccggtgtg ctctctatcc
    11881 cgctgctctt gccggatggt aacgtcttcc cggcgcgcta tgagctggtg ttcctggcgg
    11941 ctggcgtcat tctcttctcg ctgtttgtcg gcgtggtgat gttgcctatt ctgctacaac
    12001 acattgaagt cgcggaccat tcgcaacaat tgaaagaaga acgtattgcg cgagcggcaa
    12061 cggcagaagt ggcgattgtg gcgatccaga aaatggagga gcgtctggcg gcggataccg
    12121 aagagaatat cgataaccag ctgcttacgg aggtcagttc tcgcgtcatt ggtaacctgc
    12181 gtcgtcgcgc cgatggacgt aacgacgttg aaagttccgt gcaggaagag aaccttgagc
    12241 gtcgcttccg tctggcggca ttgcgttctg aacgtgctga actgtaccac ctgcgcgcca
    12301 cgcgggagat cagcaacgaa acgctgcaaa aattactgca cgatctcgat ttgcttgaag
    12361 cgttgctaat tgaggaaaat cagtaagccg aaagcgacgt aaccaggccc gaggtaatct
    12421 gaaaaaggtg caatcttttg cacctttaac aggttagctc gggctaacat tcatgattct
    12481 aaaacaaata agttatctct cttgtaaagt caattcattg gctgttcttc gacttaacct
    12541 caatctgcgt gttgcataat atctgcagta atcttcatag cgactgcatt aatattatcc
    12601 tcagtttgtg acatgccagt atgagaggta caatcttctt caaagaattt ctgtagtgtt
    12661 accagggtat cactatcaat ggggatattt tcctccccac ggagaataga aatgcccatt
    12721 ccctggacgc tagcctcatc aaaattaacc cgatagagac gggtattaaa catacaggct
    12781 tttgttaaaa tagaacagtt gaaatggcag ttgcttaaat tggcattatt aaaattaaca
    12841 ttagccatat tagagccggt acatataacc atttcgagga atgtatttgt tagccacgta
    12901 caaaagagtt tgctatcaat taagtttata ttctggagga ttgctttgtt cagattgacg
    12961 cgagacagat cagcatgttc aaagttaatt aaatcgagtt tagagaaact aaggtcggct
    13021 ttaaataaat taacatatgg cgctattact ttataaagat ttacctcagc catgtac
//
LOCUS       DAZGTR010000115        13056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGTR010000115 DAZGTR010000000
VERSION     DAZGTR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.117"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            150..1259
                     /gene="livK"
                     /locus_tag="ACHLCY_003789"
     CDS             150..1259
                     /gene="livK"
                     /locus_tag="ACHLCY_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP0370961.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1307..2233
                     /gene="livH"
                     /locus_tag="ACHLCY_003790"
     CDS             1307..2233
                     /gene="livH"
                     /locus_tag="ACHLCY_003790"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP0370962.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2230..3507
                     /gene="livM"
                     /locus_tag="ACHLCY_003791"
     CDS             2230..3507
                     /gene="livM"
                     /locus_tag="ACHLCY_003791"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP0370963.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVV
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3504..4271
                     /gene="livG"
                     /locus_tag="ACHLCY_003792"
     CDS             3504..4271
                     /gene="livG"
                     /locus_tag="ACHLCY_003792"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP0370964.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4273..4986
                     /gene="livF"
                     /locus_tag="ACHLCY_003793"
     CDS             4273..4986
                     /gene="livF"
                     /locus_tag="ACHLCY_003793"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP0370965.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5382..6698
                     /gene="ugpB"
                     /locus_tag="ACHLCY_003794"
     CDS             5382..6698
                     /gene="ugpB"
                     /locus_tag="ACHLCY_003794"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP0370966.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6796..7683
                     /gene="ugpA"
                     /locus_tag="ACHLCY_003795"
     CDS             6796..7683
                     /gene="ugpA"
                     /locus_tag="ACHLCY_003795"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP0370967.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7680..8525
                     /gene="ugpE"
                     /locus_tag="ACHLCY_003796"
     CDS             7680..8525
                     /gene="ugpE"
                     /locus_tag="ACHLCY_003796"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP0370968.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRVL
                     VDSEK"
     gene            8527..9597
                     /gene="ugpC"
                     /locus_tag="ACHLCY_003797"
     CDS             8527..9597
                     /gene="ugpC"
                     /locus_tag="ACHLCY_003797"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP0370969.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9594..10337
                     /gene="ugpQ"
                     /locus_tag="ACHLCY_003798"
     CDS             9594..10337
                     /gene="ugpQ"
                     /locus_tag="ACHLCY_003798"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0370970.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLWILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10324..10764)
                     /locus_tag="ACHLCY_003799"
     CDS             complement(10324..10764)
                     /locus_tag="ACHLCY_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP0370971.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10884..12626
                     /gene="ggt"
                     /locus_tag="ACHLCY_003800"
     CDS             10884..12626
                     /gene="ggt"
                     /locus_tag="ACHLCY_003800"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP0370972.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12664..12948)
                     /locus_tag="ACHLCY_003801"
     CDS             complement(12664..12948)
                     /locus_tag="ACHLCY_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP0370973.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2894 a         3330 c         3745 g         3087 t
ORIGIN      
        1 aatagggatt ttaatcacta ttatcacaaa atactgcgct aaacccttaa tcagacaggc
       61 aaaaacagtg cagtataaaa aaagaacagt ctgatttgtt aacacataaa aacaaagcaa
      121 cacaacatca cgaatgggga tttttgacta tgaaacggaa tgcgaaaact atcatcgcag
      181 ggatgattgc actggcaatt tcacacaccg ctatggctga cgatattaaa gtcgccgttg
      241 tcggcgcgat gtccggcccg attgcccagt ggggcgatat ggaatttaac ggcgcgcgtc
      301 aggcgattaa agacattaat gccaaagggg gaattaaggg cgacaagctg gttggcgtgg
      361 aatatgacga cgcctgcgac ccgaaacaag ccgttgcggt cgccaacaaa atcgttaatg
      421 acggcattaa atacgttatt ggtcatctgt gttcttcttc tacccagcct gcgtcagata
      481 tctatgaaga cgaaggtatt ctgatgatct cgccgggagc gaccaacccg gagctgaccc
      541 aacgcggtta tcaacacatt atgcgtactg ccgggctgga ctcttcccag gggccaacgg
      601 cggcaaaata cattcttgag acggtgaagc cccagcgcat cgccatcatt cacgacaaac
      661 aacaatatgg cgaagggctg gcacgttcgg tgcaggacgg gctgaaagct gctaacgcca
      721 atgttgtttt cttcgacggc attaccgctg gcgagaaaga tttctccgcg ctgatcgccc
      781 gcctgaaaaa agaaaacatc gacttcgttt actacggcgg ttactacccg gaaatggggc
      841 agatgctgcg ccaggcccgt tccgttggcc tgaaaaccca gtttatgggg ccggaaggtg
      901 tgggtaatgc gtcgctgtcg aatattgctg gcgatgctgc cgaaggcatg ttggtcacca
      961 tgccaaaacg ctatgaccag gatccggcaa accagggcat cgttgatgcg ctgaaagcag
     1021 acaagaaaga tccgtccggg ccttatgtct ggatcactta cgcggcggtg caatctctgg
     1081 caacagccct tgagcgtacc ggcagcgatg agccgctggc gctggtgaaa gatttaaaag
     1141 ctaacggtgc aaacaccgtg attgggccgc tgaactggga tgaaaaaggc gatcttaagg
     1201 gatttgattt tggtgtcttc cagtggcacg ccgacggttc atccacggca gccaagtgat
     1261 catcccaccg cccgtaaaat gcgggcgggt ttagaaaggt taccttatgt ctgagcagtt
     1321 tttgtatttc ttgcagcaga tgtttaacgg cgtcacgctg ggcagtacct acgcgctgat
     1381 agccatcggc tacaccatgg tttacggcat tatcggcatg ataaacttcg cccacggcga
     1441 ggtttatatg attggcagct acgtctcatt tatgatcatc gccgcgctga tgatgatggg
     1501 cattgatacc ggctggctgc tggtggcggc gggattcgtc ggcgcaatcg tcattgccag
     1561 cgcctacggc tggagtatcg aacgggtggc ctatcgcccg gtgcgtaact ctaagcgcct
     1621 gattgcgctc atctcggcaa tcggtatgtc catcttcctg caaaactacg tcagcctgac
     1681 cgaaggttcg cgtgacgtgg cgctgccgag cctgtttaac ggtcagtggg tggtgggcca
     1741 tagcgaaaac ttctctgcct ctattactac catgcaggcg gtgatctgga ttgtcacctt
     1801 cctcgccatg ctggcgctga cgattttcat tcgctattcc cgtatggggc gcgcgtgtcg
     1861 tgcctgcgcg gaagatctga aaatggcgag tctgcttggc attaacaccg accgggtgat
     1921 tgcgctgacc tttgtgattg gcgcggcgat ggcggcggtg gcgggtgtgc tgctcggtca
     1981 gttctacggc gtaattaacc cctacatcgg ctttatggcc gggatgaaag cctttaccgc
     2041 tgcggtgctc ggtgggattg gcagcattcc aggggcgatg attggtgggc tgatcctggg
     2101 gattgcggag gcgctctctt ctgcctatct gagtacggaa tataaggatg tggtttcatt
     2161 tgccctgctg attctggtgc tgctggtgat gccgaccggt attctgggtc gcccggaggt
     2221 agagaaagta tgaaaccgat gcatattgca atggcgctgc tctctgccgc gatgtttttt
     2281 gtcctggcgg gcgtctttat gggcgtgcaa ctggagctgg atggcaccaa actggtggtc
     2341 gacacggctt cagacatccg ttggcagtgg gtgtttatcg gcacggcggt ggtctttttc
     2401 ttccagcttt tgcgaccggc tttccagaaa gggctaaaaa gcgtttccgg accgaagttt
     2461 attctgcccg cgattgatgg ctccacggtg aagcagaaac tattcctcgt ggcgctgctg
     2521 gtgcttgcgg tggtgtggcc gtttatggtg tcgcgtggga cggtggatat cgccaccctg
     2581 accatgatct acattatcct cggcctcggg ctgaacgtgg ttgttggtct ttctggtctg
     2641 ctggtgctgg ggtacggcgg cttttacgcc atcggcgctt acacttttgc gctgctcaat
     2701 cactattacg gcctgggctt ctggacctgt ctgccgattg ccgggttaat ggcagcggcg
     2761 gccggcttcc tgctcggctt cccggtgctt cgcttgcgcg gcgactatct ggcgatcgtt
     2821 accctcggtt tcggcgaaat tgtgcgcata ttgctgctca ataacaccga aattaccggc
     2881 ggcccgaacg gaatcagtca gatcccgaaa ccgacactct tcggactcga gttcagccgt
     2941 accgctcgtg aaggcggctg ggacacgttc agtaatttct ttggcctgaa atacgatccc
     3001 tccgatcgtg tcatcttcct ctacctggtg gcgttgctgc tggtggtgct aagcctgttt
     3061 gtcattaacc gcctgctgcg gatgccgctg gggcgtgcgt gggaagcgtt gcgtgaagac
     3121 gagatcgcct gccgttcgct gggcttaagc ccgcgtcgta tcaagctgac tgcctttacc
     3181 ataagtgccg cgtttgccgg ttttgccgga acgctgtttg ctgcgcgcca gggctttgtc
     3241 agcccggaat ccttcacctt tgctgaatcg gcgtttgtac tggcgatagt ggtgctcggc
     3301 ggtatgggat cgcagtttgc ggtgattctg gcggcaattt tgctggtggt atcgcgcgaa
     3361 ttgatgcgtg atttcaacga atacagcatg ttaatgctcg gtggtttgat ggtgctgatg
     3421 atgatctggc gtccgcaggg cttgctgccc atgacgcgcc cgcaactgaa gctgaaaaac
     3481 ggcgcagcga aaggagagca ggcatgagtc agccattatt atctgttaac ggtctgatga
     3541 tgcgcttcgg cggcttgctg gcggtgaaca acgtcaatct tgaactgtac ccgcaggaga
     3601 tcgtctcgtt aatcggccct aacggtgccg gaaaaaccac ggtttttaac tgcctgaccg
     3661 gattctacaa acccaccggc ggcactattt tactgcgtga tcagcatctg gaaggtttac
     3721 cggggcagca aattgcccgc atgggcgtgg tgcgtacctt ccagcatgtg cgtctgttcc
     3781 gtgaaatgac ggtgattgaa aacctgctgg tggcgcagca tcaacaactg aaaaccgggc
     3841 tgttctctgg cctgttgaaa acgccttctt tccgtcgcgc ccagagcgaa gcgctcgacc
     3901 gcgccgccac ctggcttgag cgcattggtt tgctggaaca cgccaaccgt caggcgagta
     3961 acctggccta tggtgaccaa cgccgtctgg agattgcccg ctgcatggtg acgcagccgg
     4021 agattttaat gctcgacgaa ccggcggcag gtcttaaccc aaaagaaacc aaagagctgg
     4081 atgagctgat tgccgaactg cgcaatcatc acaacaccac tatcttgttg attgaacacg
     4141 atatgaagct ggtgatgggg atttcagacc gaatttatgt ggtcaatcag gggacgccac
     4201 tggcaaacgg tacgccggag caaatccgta ataacccgga cgtgatccgt gcctatttag
     4261 gtgaggcata agatggaaaa agtcatgttg tcctttgaca aagtcagcgc ccactacggc
     4321 aaaatccagg cgctgcatga ggtgagcctg catatcaatc agggcgagat tgtcaccctc
     4381 attggcgcga acggggcggg gaaaaccacc ttgctcggca cgttatgcgg cgatccgcgc
     4441 gctaccagcg ggcgaattgt gtttgatgat aaagacatta ccgactggca gacggcgaaa
     4501 atcatgcgcg aagcggtagc gattgtcccg gaagggcgtc gcgtcttctc gcggatgacg
     4561 gtggaagaga acctggcgat gggcggcttt tttgccgaac gcgaccagtt ccaggagcgc
     4621 ataaagtggg tgtatgaact gtttccacgt ctgcatgagc gccgtgttca gcgggcaggc
     4681 accatgtccg gcggtgagca acaaatgctg gcgattggtc gcgcgctgat gagcaacccg
     4741 cgtttgctgc tacttgatga gccatcgctc ggtcttgcgc cgattatcat ccagcaaatt
     4801 ttcgacacca tcgagcagct gcgcgagcag gggatgacta tcttcctcgt cgagcagaac
     4861 gccaaccagg cgctaaagct ggcggatcgc ggctacgtgc tggaaaacgg ccatgtggtg
     4921 ctctccgata ccggcgacgc gctgctggcg aacgaagcag tgagaagtgc gtatttaggc
     4981 gggtaataac acgttgattg ataaggagtc aaaagactcc tttgagacag gtgacaaatg
     5041 taaaattgcc tgatgcgctg cgcttatcag gcctactggg tgagttgcaa tatattgaat
     5101 ttgcacgatc ttgtaggccg gataaggcgt ttacgccgca tccggcatga aacgatgagt
     5161 aatctgtaga gtttgattca gaccttctat tttccgctta tccgtgcccc atctcccatt
     5221 ttccctcacc cacgccgtca ccgccttgtc atctttctga caccttacca tcttacaaat
     5281 gtaacaaaaa agttattttt ctgtaattcg agcatgtcat gttaccccgc gagcataaaa
     5341 cgcgtgaatt cgcgcattcg gtacaacaag agagataaac gatgaaaccg ttacattata
     5401 cagcttcagc actggcgctc ggactggcgt taatggggaa tgcacaggca gtgacgacca
     5461 ttccgttctg gcattctatg gaaggggaac tgggtaaaga ggtggattct ctggcccaac
     5521 gttttaacgc cgaaaatccg gattacaaaa ttgtaccgac ctataaaggc aactacgaac
     5581 agaatttaag cgcggggatt gccgcatttc gtaccggcaa cgctccggct attttgcagg
     5641 tttatgaagt tggcaccgcc accatgatgg catcgaaagc cattaaaccg gtatatgacg
     5701 tgtttaaaga ggcggggatt caattcgatg agtcgcagtt tgtgccgacg gtttccggtt
     5761 actactccga cagcaaaacg ggccacttac tctcccagcc attcaacagc tcgacccccg
     5821 ttctctatta caacaaagac gccttcaaga aagcaggatt agacccggaa caaccgccga
     5881 aaacctggca ggatctggcg gactatgccg cgaaactgaa agcctccggt atgaagtgcg
     5941 gctacgccag cggctggcag ggctggatcc aactggaaaa ctttagcgcc tggaacggtc
     6001 tgccgtttgc cagcaaaaac aacggctttg acggcacgga cgcggtgctg gagttcaaca
     6061 agccggagca ggtgaaacat atcgccatgc tcgaggagat gaacaagaag ggcgacttca
     6121 gctacgtcgg tcgtaaggat gaatccaccg agaagttcta taacggtgat tgcgcgatga
     6181 ccaccgcctc ttccggttct cttgccaaca ttcgcgagta cgccaaattt aattacggcg
     6241 taggcatgat gccttacgac gccgatgcga aagatgcgcc acaaaacgcc attatcggcg
     6301 gagccagcct gtgggtgatg cagggtaaag ataaagaaac gtataccggt gtggcgaagt
     6361 tcctcgattt cctcgcgaag ccagaaaacg ctgccgagtg gcatcagaaa accggttatc
     6421 tgccaattac caaagcggcg tatgacctga cccgtgagca gggcttttac gagaaaaacc
     6481 caggtgcgga taccgcgacg cgtcagatgc tgaataagcc gccgttgccg ttcaccaaag
     6541 ggctgcgtct gggcaacatg ccgcagatcc gcgtgattgt ggatgaagag ctggagagcg
     6601 tgtggaccgg taagaagaca ccacagcagg cactggatac cgccgttgag cgtggaaatc
     6661 agttgctgcg ccgctttgag aaatcgacga agtcttaatc agtgtaatgt cggatgcgtt
     6721 tcgcttatct gacctggcat cgcgtgtagg ccggataagc gaagcgcatc cggcacagtt
     6781 caggaattaa ccgtaatgtc atcatcccgt ccggtgttcc gctcgcgctg gctgccttat
     6841 ctgctggtcg cgccgcagct catcatcacc gttatctttt ttatctggcc tgcgggcgaa
     6901 gcgttgtggt actcgctaca aagcgtcgat ccgtttggtt tctccagcca gtttgtcggc
     6961 ctggataact tcgtcgcgct gtttcatgac agctactatc tcgactcctt ctggacgacg
     7021 ataaaattca gcacctttgt caccgtcagc ggtttgctgg tgtcgctgtt cttcgcggcg
     7081 ctggtggagt acatcgtgcg cggcagccgt ttctatcaaa ccttaatgct gctgccttac
     7141 gccgtggctc ccgccgttgc cgccgtattg tggatcttcc tgtttaaccc cggtcgcggg
     7201 ctgatcaccc attttctcgc ggagttcggc tacgactgga accacgcgca aaacagcggt
     7261 caggcaatgt ttctggtggt gtttgcctca gtatggaagc aaatcagcta caacttcctg
     7321 ttcttctatg ccgcgctgca atccattccc cgttcgttga tcgaagccgc agccatcgac
     7381 ggtgcaggcc caattcgccg cttctttaag attgcgctgc cgcttatcgc tccggtgagt
     7441 ttcttcctgc tggtggtgaa cctggtgtat gccttcttcg acaccttccc ggtgatcgac
     7501 gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa gatctaccgt
     7561 gaaggcttta ccggactgga tctggcttcg tctgccgcgc agtcggtggt gttgatgttc
     7621 ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt gcgttaccaa
     7681 tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc ctcgggatcg
     7741 cggtgatcct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat aaacaggccg
     7801 tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaat atccacaaca
     7861 tctgggttaa cggggtaggc acgaatagcg cgccgttctg gcggatgttg cttaacagct
     7921 ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc tcggcatttg
     7981 ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt tttatcaccc
     8041 tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc aacctgaaga
     8101 tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc gccacgtttc
     8161 tgtttcgcca gttctttatg acgctgccgg atgaactggt ggaagcggcg cggatcgacg
     8221 gcgcgtcacc aatgcgtttc ttttgcgaca tcgtttttcc gctctccaaa accaatctgg
     8281 cggcgctgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg ccgttgttga
     8341 ttattaccga tgtggatctc ggcaccaccg tggcagggat caaagggatg atcgctacag
     8401 gcgaaggcac cactgaatgg aactcagtga tggcggcgat gttgttaacg cttatccctc
     8461 cggtggtgat tgttttagtg atgcagcgtg ccttcgtgcg cgtcctggtc gatagtgaga
     8521 aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg taaaacccag
     8581 gtcattaaac cgctgaccct tgatgtggcg gatggcgaat ttatcgtgat ggtcgggccg
     8641 tctggctgcg ggaaatcgac gctgctgcgc atggttgccg ggctggagcg ggtgacagaa
     8701 ggcgatatct ggatcaacga ccagcgcgtg actgaaatgg agccaaaaga tcgcgggatt
     8761 gcgatggtgt tccagaacta cgcgctttat ccgcatatga gtgtcgaaga aaacatggcg
     8821 tgggggctga aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt taaagaagcg
     8881 gcgcgcattc tggagctgga cggtctgctc aaacgtcgcc cgcgcgagct ttccggcggt
     8941 cagcgccagc gtgtggcgat gggccgcgcg attgtgcgcg atccggcggt gttcctgttt
     9001 gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgtct tgaactgcaa
     9061 cagttgcacc gtcgcctgaa aacgacttca ctctacgtta ctcacgatca ggttgaagcg
     9121 atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca gattggcaca
     9181 ccagttgaag tctacgaaaa gcccgccagc ctgtttgtag cgagttttat cggcagtccg
     9241 gcgatgaacc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga actggacggc
     9301 ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa aatgactctc
     9361 ggcattcgcc cggaacatat tgcgctaagc tcgcaggcag aaggcggcgt accgctggtg
     9421 atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg gggcgagcag
     9481 aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac gctgtggctg
     9541 catctggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg agtatgagta
     9601 actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc ccggaaaaca
     9661 ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa tttgacgcga
     9721 agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa cgcaccagca
     9781 acggctgggg cgtcgcgggt gaactgaact ggcaggattt actgcgcgtg gatgcgggca
     9841 gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag gtggcggaac
     9901 gctgtcgcga acacgggatg atggcgaata tcgaaatcaa acccaccact ggcactggac
     9961 cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt atgacgccgc
    10021 cgctgctgtc atcgtttgag attgatgctt tagaagctgc acagctggcg gcaccggaac
    10081 tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg accgcgcggc
    10141 tgggctgcgt ctctattcat ctcaatcata agttactcga taaagtgcga gtgatgcagt
    10201 tgaaagacgc cggactgtgg attctggttt ataccgtcaa taaaccccag cgcgcggcag
    10261 agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg attggtccga
    10321 actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg gctgattcag
    10381 catgtcgccg tttctcctct ccggcagcat atgctgctga ttcaacgaac tatccggatt
    10441 acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctgcc gggcgggatt
    10501 tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct ggttttctaa
    10561 atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc ctttttgctg
    10621 gatttgctga gtctgcatct gggtttgcat ccgctgctgg ctgggtatct gataccccgg
    10681 ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg gcaggagtgc
    10741 cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct ttaagtttac
    10801 tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag ctggggttgc
    10861 cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg gtggccattg
    10921 ctgctctgct ctcaggaagt tgttttagcg ccgccgccgc gcctcctgcg ccgcccgtct
    10981 cgtatggtgt ggaggaagat gtcttccacc cggtacgcgc gaaacaggga atggtagcgt
    11041 ctgtggacgc cactgccact caggtggggg tggatattct caaggagggc gggaatgccg
    11101 ttgatgccgc cgtggcggtg ggctacgcgc tggcggtaac gcatccgcag gcagggaatc
    11161 tgggcggtgg tggttttatg ctgatccgct cgaaaaatgg caataccacc gctatcgatt
    11221 tccgcgaaat ggcacccgct aaagcgaccc gcgatatgtt ccttgatgac cagggcaacc
    11281 cggacagcaa aaaatcactc acttcgcatc tggcttccgg cacaccaggt acggtcgctg
    11341 gtttctcgct ggcgttggat aagtacggca ctatgccgtt gaacaaagtg gtgcagcccg
    11401 cgttcaaact ggcacgcgat ggttttatcg ttaacgatgc tttggcagac gatctcaaaa
    11461 cctacggtag cgaagtgttg ccgaatcacg aaaacagcaa agctatcttc tggaaagagg
    11521 gcgaaccgct gaaaaagggc gacacgctgg tgcaggcgaa cctggcaaag agcctggaga
    11581 tgattgctga aaacggcccg gacgaattct ataaaggcac gattgcggaa cagatcgccc
    11641 aggagatgca gaaaaacggt ggcttgatca ctaaagaaga tttagcggcc tataaagcgg
    11701 tcgaacgcac tccgataagc ggcgattatc gcgggtatca ggtttactcc atgccaccgc
    11761 catcctccgg cgggatccat atcgtacaaa tcctcaatat tctggaaaac ttcgatatga
    11821 agaaatacgg ctttggcagc gccgatgcga tgcaaatcat ggcagaagcg gagaaatacg
    11881 cctacgccga ccgctcggaa tatcttggcg acccggattt tgtcaaagtc ccgtggcagg
    11941 cactgaccaa taaagcctat gccaaatcca ttgccgatca aattgatatc aacaaagcga
    12001 agccgtccag tgagattcgc cctggcaagc ttgcgcctta tgagagtaat caaactaccc
    12061 attactctgt agtggataaa gacggtaacg cggtggcggt gacctatacg ctgaacacca
    12121 ccttcggtac gggtattgtc gcgggcgaga gcggtattct gcttaataac cagatggatg
    12181 atttctccgc caaaccgggc gtaccgaacg tttacgggct ggtgggcggt gatgccaacg
    12241 ccgtcgggcc gaacaaacgc ccgctgtcgt cgatgtcgcc gaccattgtg gtgaaagacg
    12301 gtaaaacctg gctggttacc ggtagcccag gcggtagccg gatcatcact acagtgctgc
    12361 aaatggtggt gaatagcatc gattatggca tgaacgtcgc cgaagcgacc aatgcgccgc
    12421 gtttccacca tcagtggttg ccggacgagc tgcgtgtcga aaaagggttt agcccggata
    12481 cgctcaagct gctggaagca aaaggtcaga aagtggcgct gaaagaggcg atgggcagta
    12541 cacaaagcat tatggttggg ccggacggtg agttgtacgg cgcatccgac ccgcgctcgg
    12601 tggatgattt aacggcgggg tactaaggtt agcggccctc ttcgtgggaa gagggctatt
    12661 ttgtcagggc aagccgaagg tagccttttt tatttcgtaa tcctgtagat attcttccag
    12721 cggctttgcc gttcccagag aatgaatttc accactgtct ttatcaataa taaaaggtgc
    12781 gttaccagct aagcgcgcgg cctcatctcc agtttcgaga aattctcgtg cttcgaaaca
    12841 gaaataccag ccctggctaa agcgtccatg tagagtaatg acgaccggga gatctgcatc
    12901 atcaaggtaa tggttcgctt tcgcgaatgc gtcgtgataa gtaatcataa ttataataaa
    12961 tattattgtt gagtgttata ttattattcc tgtgtgatac attgagcaaa gacgcgttca
    13021 tcttcgtcaa agatattatt aaatgctggt tttgaa
//
LOCUS       DAZGTR010000116        13049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGTR010000116 DAZGTR010000000
VERSION     DAZGTR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.118"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            90..278
                     /locus_tag="ACHLCY_003802"
     CDS             90..278
                     /locus_tag="ACHLCY_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0370974.1"
                     /translation="MVNLPPELGSPDVWAGGGIGLIAGLLLRYFIVRVFIVFRLFGRE
                     WSFDLKDRTRTDNHTDPR"
     gene            complement(1789..2337)
                     /locus_tag="ACHLCY_003803"
     CDS             complement(1789..2337)
                     /locus_tag="ACHLCY_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0370975.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRDMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVESLQTKGVRVVSLREGFDLS
                     TPIGKAILTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISKATYYRLAK"
     gene            3133..3429
                     /locus_tag="ACHLCY_003804"
     CDS             3133..3429
                     /locus_tag="ACHLCY_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP0370976.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            3728..4981
                     /gene="kch"
                     /locus_tag="ACHLCY_003805"
     CDS             3728..4981
                     /gene="kch"
                     /locus_tag="ACHLCY_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP0370977.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(5036..5209)
                     /locus_tag="ACHLCY_003806"
     CDS             complement(5036..5209)
                     /locus_tag="ACHLCY_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP0370978.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            5352..6812
                     /gene="cls"
                     /locus_tag="ACHLCY_003807"
     CDS             5352..6812
                     /gene="cls"
                     /locus_tag="ACHLCY_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP0370979.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            6847..7176
                     /locus_tag="ACHLCY_003808"
     CDS             6847..7176
                     /locus_tag="ACHLCY_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP0370980.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7229..8233)
                     /gene="oppF"
                     /locus_tag="ACHLCY_003809"
     CDS             complement(7229..8233)
                     /gene="oppF"
                     /locus_tag="ACHLCY_003809"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP0370981.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(8230..9243)
                     /gene="oppD"
                     /locus_tag="ACHLCY_003810"
     CDS             complement(8230..9243)
                     /gene="oppD"
                     /locus_tag="ACHLCY_003810"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP0370982.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(9255..10163)
                     /gene="oppC"
                     /locus_tag="ACHLCY_003811"
     CDS             complement(9255..10163)
                     /gene="oppC"
                     /locus_tag="ACHLCY_003811"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP0370983.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(10178..11098)
                     /gene="oppB"
                     /locus_tag="ACHLCY_003812"
     CDS             complement(10178..11098)
                     /gene="oppB"
                     /locus_tag="ACHLCY_003812"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP0370984.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(11184..12815)
                     /gene="oppA"
                     /locus_tag="ACHLCY_003813"
     CDS             complement(11184..12815)
                     /gene="oppA"
                     /locus_tag="ACHLCY_003813"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP0370985.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLNGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         3451 a         3132 c         2902 g         3564 t
ORIGIN      
        1 cgagttaaat ggcctcttca ggtcaactca taaggtcagg tacttttttg tgtcagaatg
       61 cgattgagag cgtcttacaa ggaaaactaa tggtaaatct ccctcctgag ctggggtcac
      121 ctgatgtatg ggctggtgga ggtattggac ttattgcagg tctgctactt cgatatttca
      181 tcgtcagagt gtttatcgtg ttcagattgt ttggtcgtga atggtcgttt gacctcaagg
      241 acaggaccag aacagacaac cacacagacc cccgttaaac ccccttcaaa accccctgta
      301 cgtgatggta taggggtatc tttatcattt tgtgctatct attaaaaaaa tatcttgaca
      361 gaaccggaaa gctgtatcta tcaagtattc acctctctga cagtaatttc taaaagttct
      421 tatttttcaa taagttgtaa ttatccatat atagagaact gacaagtctc ttcacagtta
      481 actctgtaac cttaacagca acctctaaaa acacttattt ttcaatgagt tgtaatcacc
      541 caatatataa ggctgtccag caccttcaca gagaaacttc agaggtcttc ttagttgggc
      601 ttgtataaca caacccctaa ccgtccacta agcgggagaa gagcgacacc caagaaaggg
      661 aagctccctt ctaattggta ttgttaccaa tccctaaacc gaccttctta accagccctt
      721 tacgttattc gttcacagtg ttgataagtg gttttgccct ctgtttctgt tatcatcggg
      781 cttaccttat cagggctttg aaatgccctg taactcccct cttaacggga gagaaagaac
      841 atagtgaccc caagcgaagc gcccctctaa aaagattttt tagctatttt gcttaaaaac
      901 acatcaaacg atacatttta ttgatttagg tcaaagacaa aagcattgac aaaatggtaa
      961 gattgttcac atgaccttac ttaacagatt cttaagagct ttgaaacgaa ctgtttaaca
     1021 ggtcactacc tctggtctta tcgttccctc tcttcttggt agttctgtta agaggaactt
     1081 ttcaggtatc atcttacagt cctgttaaga tagaacctac cttcggtttc atggttctct
     1141 tcctcccgtt aagcaattct tcctaacaac ttcatagtta tctgtcaagt acctttgaag
     1201 attctatcaa agccctctaa atctgacctg atatagatgc tgtgaaggca tgttctctag
     1261 gtctgtgtag cttacttagt gggaaaagag gatatggtga ccatagggga acccctcctg
     1321 ttaaaagttt cttgatgtta tcatcacaat atatataatc accaactgta cagacacctt
     1381 aacggtttct ctacacatcc ctatggctag gtagggtgtg gtttagcttg gtacggctag
     1441 gttaggtctg gtttggttag gtatgggtgg attgtcgtag ccacgttaaa aatcgactat
     1501 gaggtttatt tactaaaccg aatcaactat gagggactct tcatgaagta attcattact
     1561 gaattaccta cgggagtcct ttttagtttg tataaaatct gtacaggagt tatcaggctt
     1621 ctaaaatttt tataaaaaat aatcagacct cttctaagtc tcttcaacca aatctttcaa
     1681 aaatatctga cagatgtctg tagctgctaa gcacctgtta caccagctat gtgaccccct
     1741 ttgcaaaaaa gccaaatatc agtcacatcc atgtaactgt tcagtaactt atttagcaag
     1801 tctgtaataa gttgctttag agattcctag ctcttcctga atctgcaacc gtgtcttacc
     1861 ttgtgaaata agcgtctgta cagcctctga agaagctttt acaggcctcc ccatatgtac
     1921 cccttcagct ttagcacgtt ctattccagc ctttctacgt tcactgataa ggtctttttc
     1981 aaggtcagct aaaccagcca tcatagtaag tattgcttta ccgattggtg tagacaggtc
     2041 aaaaccttca cgtagagaaa caaccctcac acctttagtc tgtagtgact ccacgttaga
     2101 gagcacatca acggtattac gccctaaacg gtcaatagct accacaacta acgtatcgtc
     2161 acaccttaca tagttcatca tgtctctaaa accatctctg ctggtggcct ttgtagctcc
     2221 tgagacagcc tcatcaataa accatttatc gaccttgtga accttctcta tctgtagttt
     2281 ctggttgtct acagtctggt cttgtgttga taccctgcaa taaccaatca ccgccataca
     2341 tcccccttag atgtctcaaa agttagtctc ataagtttag ctgtctcata atccaaaaac
     2401 aaccttttga gactacttga gggtgcattc taccgttacg ttgaaagtgt ctcaaaagga
     2461 agtcctttta agactggtta tgaaggagct atgaggttga taccgaatca actatgaaga
     2521 ttttaggagg gtaactataa ccatcacttt ctaactttca cgcaggaaac tgttaagttt
     2581 cttgatagtt ataccatgtg accaactgca tagatagcgt tatatatccc tgtatatagc
     2641 acttttctag gagtcgagag aggaacgact aacacgatac agtttatcct gaaaagggac
     2701 ttcacagatt ctctatatat ttcaataagt taggataaaa agagactata ccgatactgc
     2761 atagaatctg tgtaaaactg cttagatact tggaaatcta ttattagatt gcatagtcat
     2821 aggggttgga acaagcccaa ctacacctga tattgtcctg tttttaacag gtctcttaga
     2881 aaaccgaata gacttcattc aagggtcatt gaagtcataa tcatttcagt agaaaaacca
     2941 ggtctcgatt ttaaatgcaa atagcaatca tattcaataa ggcaagacga tcaggatcag
     3001 tgaaaatgtc atttttggcg taattgacct taatttatgc atggtcttaa caaagacatc
     3061 atctttccgt tgcagatctt cgccctttca aataacgtac tttacaactt tcccgaacaa
     3121 ggagttgtgc ccgtgttgta tgttatttac gctcaagaca aagctgatag cctcgaaaaa
     3181 cgcctttccg ttcgtccggc acatttagca cgtttacagt tactgcatga tgaaggtcgt
     3241 ttgttgacag ctggtccaat gccagcggta gacagtaacg atccaggggc tgcgggattt
     3301 actggctcaa ccgttattgc tgaatttgaa tcgctggaag cagctcaggc ctgggccgat
     3361 gctgacccgt acgtagcggc aggagtctat gaacacgttt cagtgaaacc atttaagaaa
     3421 gtgttctgaa tagagattca atatttaagg gcacggtttt tgcaaccgtg ttcttgtttg
     3481 gcctttcctt atagccggtc agtgcattca ccaccacaag cagataataa ctttatctgt
     3541 atttctttat atgatgtgaa catattcagg aaaatgccct ttcctgacgc agagttgctc
     3601 gctcaaaacc tttgattact gccgataaag tcatctacta ttgtttaaca cattcacgcg
     3661 ctgcatgtat gttcagaaaa tgtgcacgct aagataatta aacaaaattg tggttacaga
     3721 ggtatcagtg agtcactggg ctacattcaa acaaacagca acaaatttat gggtgaccct
     3781 tcggcacgac attctcgcgc tggccgtctt tttaaatgga ttgcttattt ttaaaacaat
     3841 ctatggtatg tcggtcaatt tgcttgatat tttccatatc aaagcatttt cagagttgga
     3901 tctctccttg ctggcaaacg ccccactatt tatgctcggc gtctttcttg tcctgaactc
     3961 cattggctta ctgttccggg caaagctcgc atgggcaatc agtatcattt tgttgttgat
     4021 agcgctaatt tacaccctgc atttttatcc ctggctgaaa tttagtattg gattttgcat
     4081 ttttacgctg gtgtttttgc tgatactgcg caaagacttc tcccacagta gcgccgcagc
     4141 cgggacaatt tttgcattta ttagtttcac gacattactg ttttactcaa cctacggtgc
     4201 gctttattta agcgaaggtt ttaatccgcg aatagaaagt ttgatgaccg cgttctattt
     4261 ttcgatagaa accatgtcaa ccgtcggcta tggcgatatt gtccctgttt ctgaatcagc
     4321 acgattgttc actatttcgg tcattatttc cggcattacc gtttttgcca catctatgac
     4381 ctcaattttt ggtccgctta tccgcggggg attcaacaaa cttgtaaaag gaaacaatca
     4441 tacaatgcat cgtaaagatc attttattgt ttgcggacat tcgattctcg ccatcaatac
     4501 gatcctgcaa ctgaatcaac gcggacaaaa cgtaacggtt atcagcaact tgcctgaaga
     4561 tgatatcaag caacttgagc aacgcttagg cgataacgct gatgttatcc ccggtgacag
     4621 taatgacagt tcagtattaa agaaagcggg aatcgatcgt tgccgggcca ttctggcgct
     4681 gagtgataac gatgcagata acgcgtttgt tgtactctcg gcaaaagata tgagcagtga
     4741 tgtcaaaaca gttctcgccg tcagtgatag caaaaacctg aataagatta agatggtaca
     4801 tccggatatc attctctcac cgcaactgtt tggcagcgaa attctggcgc gagttttaaa
     4861 tggtgaagag attaataatg atatgctcgt ttcaatgttg ttgaactccg gtcatggcat
     4921 tttcagcgat aacgatgaac aagaaacgaa agctgacagt aaagaatcag tgcaaaaata
     4981 gagtttaaag acgaaagagt aaacttattg ggcaccgcaa tggtgcccag tatatttaga
     5041 tattgtagat cgcaaacaat aacgagttac gctgtttgtt tagaatgcac ttcctgatgc
     5101 tgtggatacg catatttcgg cgcgattgcc cctcaagcca acgcgattta cgtcggctag
     5161 cctgacgctg catgcgccag cgccccactt ccgttctact ccgcttcatg ttaactactc
     5221 tatgcaataa cagaatggtc attatacccc cacccccgac gctgaccagt gcttttcccg
     5281 cgtttttatt tgccagatga atccatatgc gtaaactcat aacaatgcgc tttcaaaagg
     5341 atttctaatc tatgacaacc gtttatacgt tggtgagttg gttggccatt ctgggatact
     5401 ggttgctcat tgcaggcgta actttacgca ttctaatgaa acgacgcgca gttccctccg
     5461 cgatggcctg gctgttgatt atttacattc tgccgttagt cggaattatt gcctatcttg
     5521 ccgttggcga gctccattta ggcaaacgcc gcgctgagcg cgccagagcg atgtggcctt
     5581 ccaccgcaaa atggcttaac gaccttaaag cctgtaagca tatcttcgcc gaagaaaata
     5641 gcagtgtcgc tgcgccatta ttcaagcttt gcgagcgtcg tcaggggatc gctggggtca
     5701 aagggaatca gctacaactg atgaccgagt cagatgatgt gatgcaggcg ttaatccgcg
     5761 acatccagct cgcgcgccat aatattgaga tggtgtttta tatctggcag cccggcggca
     5821 tggcggacca ggtggctgaa tcattaatgg cggctgcacg gcgtggtatt cattgccgat
     5881 tgatgctcga ctccgccggg agtgtggctt ttttccgcag cccgtggccc gagctaatgc
     5941 gtaatgccgg tattgaagtg gtcgaagcct taaaggtcaa tctgatgcgt gtgtttttac
     6001 gccgtatgga cctgcgccaa catcgcaaga tgatcatgat cgataattac atcgcgtaca
     6061 ccggcagcat gaatatggtc gatcctcgct acttcaaaca agatgcgggc gtagggcaat
     6121 ggattgatct gatggcgcgt atggaaggcc ccatcgccac cgcgatgggg attatttatt
     6181 cctgcgactg ggagattgaa accggaaaac gtattctgcc gccaccacca gatgtcaata
     6241 ttatgccgtt tgaacaggcc agcggtcaca ccattcacac aattgcttct ggccccggct
     6301 ttccggaaga tctcattcac caggcattat tgactgcggc ttattcggcg cgtgaatatt
     6361 tgatcatgac cacgccctac tttgtgccaa gcgatgattt acttcatgcg atttgcacgg
     6421 cggcgcagcg cggggtggat gtcagtatta tccttccgcg aaaaaatgac tcgatgctgg
     6481 tcggctgggc cagtcgcgca ttctttacgg aactgcttgc tgctggggtt aaaatttatc
     6541 agtttgaagg cgggttactg cataccaaga gcgtgctggt cgatggcgaa ctaagtctgg
     6601 ttggcaccgt taaccttgat atgcggagtc tgtggctgaa tttcgagatt accctggcaa
     6661 tcgacgataa aggttttggt gcagaccttg ccgccgttca ggacgattat atttcgcgtt
     6721 cacgtctgct cgatgcccgt ttatggctaa aacgtccatt atggcaacgt gtcgccgagc
     6781 gactgtttta cttcttcagt ccgttgctgt aaaacgtcgc caacagaggt taaacaggta
     6841 gtgactatgg atatggatct aaacaatcgc ctgactgaag atgaaacgct tgagcaggcc
     6901 tacgatattt ttctcgaact ggctgccgac aatctcgatc cagctgatgt tctgctgttc
     6961 aatcttcagt ttgaagagcg cggcggcgcg gagttattcg atccggcgga agactggcag
     7021 gaacatgttg atttcgattt gaacccagac ttttttgccg aagtggtgat tggtctggcg
     7081 gacagtgaag atggtgaaat caacgatgtt ttcgcccgca ttctgttatg ccgcgaaaaa
     7141 gatcacaaac tttgccatat catctggcgg gaataaataa tcgcttttag cgtgaaaaag
     7201 ggctgacaac tgtcagccct tattgtgttt aaagcggatc gactttcagg caagaaacgg
     7261 cgtgtctgaa actcccctcc agaacaggac gtgttttggc gcactccgga ccggcaatcg
     7321 ggcaacgggt acggaaaaca caaccggaag gcgggttgat cggcgacggt aattcccctt
     7381 ccagtaactg gatggttttg ttcttctcca gatccggatc aggtatgggg actgccgaca
     7441 tcaatgccct ggtgtaagga tgtagtggat tgtggtagac ctcatcatag gtccccagtt
     7501 ctaccgcatg gccgagatac atcaccaaca cacgatcgga aatgtgtttt accacggcca
     7561 ggtcatgagc gataaaaatt aatgacaatc ccatctcacg ttgcagctgc tggagcaggt
     7621 tgaccacctg cgcctgaatt gacacgtcca gcgccgaaac cggctcatcg cagataatca
     7681 gcttcggttc aagaataaga gcacgagcaa tcccgatacg ctggcactgc ccaccggaga
     7741 actcatgcgg atagcggtta atcaggttag gcaataaccc gactttcagc atcatcgcct
     7801 tcacgcgctc gcgaacttcc tggcgtgaca ttttcggatg ataagtacgc agtggttcag
     7861 cgatgatctc gccgatggtc atacgcgggt ttagcgatgc caacggatcc tggaaaatca
     7921 tctgaatatc actgcgaacg gcacgccatt catcgggctt catgcccagc aactctttac
     7981 ctaaccaggc aacatgaccg tcggtcgcct tgaccaaacc gatgatggcg cgagcaaagg
     8041 tggacttacc gcatcccgat tcccctacca cacctaatgt ttccccttca tacaggcgaa
     8101 gagtgacacc atcgacggct ttgagcgttt tcggcggttg ccagaaccac tgtttgccat
     8161 ctttgatttc aaagtgcact ttaagatcgg cgatttcaag gaggactttt cttccttcag
     8221 ttacagcatt cataacagtt cctccaccgg tttaaagcaa gcacgcagac ggccaggcgt
     8281 aaactcttcc agcggcggtg cgctactaca aatttccatc gcatgtggac aacgtggctg
     8341 gaacgggcaa cctttcggta atcgcagcaa gtttggcgga ttacccggga tggtcaacat
     8401 tgtttcacct tccgcatcaa gacgcggcac cgcgttgagc aaaccgatag aataaggatg
     8461 aacgggttga taaaagacat cgcgcgcatt gccatattcc atcgtgcgcc cggcgtacat
     8521 taccagcact ttgtcacaga tccccgccac cacgccaaga tcgtgggtga tcataatgat
     8581 ggcggtatta aattcccgct tcagttcatt taatagcgtc atgatctgcg cctgtacggt
     8641 gacgtccagc gcagtagtgg gttcatccgc aatcagtagc ttaggtcgac atagcaatgc
     8701 catcgcaatc atgactcgct gacgcatgcc gccagaaaat tcgtgcgggt acattttcat
     8761 gcgtttacgc gcttccggca tttttaccgc atcgagcatc cgcaccgact cttcaaacgc
     8821 ctcagctttg ctcatgttct tatgcagcat cagcacttcc attaactgct caccgacgcg
     8881 catataggga ttcaacgaag tcattgggtc ctgaaaaatc attgagattt gttcagcgcg
     8941 cagtttattg agttcatgct ctggcaaatt gaggatttca cgcccattga aggttgccga
     9001 tccgccaata cgtccgttgg cagccaacag gcccatcaac gcaaatgcag tttgcgattt
     9061 acccgaacca gactcaccta caatacccag cgtttctccg gcacgtaggg aaaaattcaa
     9121 atcattgacc gccgtgacgt cgccgtccgg ggtactaaag gtgacacgca aatctttcac
     9181 gttcagcagt gcgtcagcct gttgttgtgc gagcggcaca gttgcagttt caattacgct
     9241 catggctgca ctccttaacg atctttcggg tcgagggcat cacgcaagcc atcgccgata
     9301 aagttgaaac aaaacagcgt caccacgagg aatcccgctg ggaacaacaa taaccatgga
     9361 gagacttcca tcgagttcgc gccatcactc agcaatgcac cccagctgct taacggctct
     9421 tgcgtaccca accccaggaa gctaaggaaa gattcaaaga ggatcatgct gggcaccaga
     9481 agtgatgcgt agaccaccac cacaccgagt acattcggca caatgtggcg aataacaata
     9541 cccgatgtcg atacaccgcc aacttgtgcc gcctcaataa actctttgcg cttcagactc
     9601 agggtttgcc cacgcacaat acgagccata tccagccagg aaaccatgcc tatcgccacg
     9661 aaaatcagca ggatgttttg accgaaaaag gtcaccagca aaatgacgaa gaacatgaat
     9721 gggaaggagt tgaggatttc cagcagacgc atcattaccg aatccacttt accgcccaga
     9781 taaccggaaa gcgaaccata aagtgtcccg acgaccacag ccaccagtgc cgcagcaaca
     9841 ccgaccatga gtgagatacg cccgccaatc gcaacgcgca caagcaggtc gcgaccggat
     9901 gagtcagtac caaagtagtg accggactcc atatccgggg cgctggacat catcgcccag
     9961 tcagtatcgt cataggcaaa ctgggaaagc atcggtgcca ggattacaaa taacgcgatc
    10021 agcaccagca ctatcagact ggcaaccgcc gcacggttat gcataaaacg tcgacgtgcg
    10081 tcctgccaca agctgcgccc ttcgacctcc agcttttcac tgaaattttc cagcgtctcg
    10141 ctgtttttct tacttaacat catcgcgagc tccagtatca gtaacggatt ttcgggtcga
    10201 taaccgcata tagcacatcg acaatggcat taaacaaaat ggttaaagca ccaaccagga
    10261 tggtcaggct taacactaag gaatagtcac ggttcaatgc gccattaacg aacagttgtc
    10321 caatccccgg caaaccataa atggtttcga taaccataga accggtaata atgccgacaa
    10381 atgcggggcc catataagag agcacgggta acagagcagg ttttaatgcg tgacgtaaaa
    10441 tgatccgccg cataggtaac cctttcgccc gggcagtacg aataaaattg gagtgtaata
    10501 cttcaatcat agagccacgg gtaatacgcg caatactggc gatataagcg agtgacaacg
    10561 ccaccatcgg caatatcatg aatttaagcg ccccaccatt ccagccaccg cccggcagcc
    10621 aatgcaaaat gatcgcaaat atcatgacta ataatggtgc aaccacaaaa ctggggataa
    10681 caaccccggt cattgccagc cccatcacgg tatagtccca tttggtgttt tgttttaatg
    10741 cggcaataac gccagcacta acacccagta ttaccgcaag gaaaaatgct gcggctccca
    10801 gtttggcaga aacgggaaaa ctggatgcaa ccaagtcatt gaccgaataa tctttatatt
    10861 taaacgatgg accgaaatca ccgtgcgcca gttgtttcag gtagctgaaa tactgtgtca
    10921 tgatcggatc attaagatga tatttcgctt cgatattggc catcacttct ggcggtaaag
    10981 tacgttcgcc ggtaaaaggg cttcccggcg cgaggcgcat cataaagaac gaaatagtaa
    11041 taagaataaa tagcgtcgga atcgcttcca gacagcgacg tagaataaat tttaacattg
    11101 ccctaccttc tggcctgtgc cttctgtaat gcgataaaaa tcagacaccg tggagcagga
    11161 cactcctgcc ccacgtattg ccattagtgc ttcacaatgt acatattccg ggtataggta
    11221 ttatccagcg gatctttgcc ggtatagcca ccaacccacg gtttcaccag acgcgcattc
    11281 acgtagtaat aaacaggaac aatggccgaa tccttatcca gctgttgttc tgctttagtg
    11341 tacagagctg tgcgctgcgc ctcgtcagtc actttcagcg tttccgccat aatgctgtca
    11401 aaggccgggc tcttataatg cgcggtattc atcgagctgt tcgaaagcat ggtgttcagg
    11461 aaggaagttg gttcgttgta gtcagcacac cagcctgcac gggccacatc aaaagtaccc
    11521 tggtgacggg tgtcgaggaa cgttttccac tcctggttaa ccagtttgac gtttacacca
    11581 atgtttttct tccacaatga agaggcagca atcgccagct ttttatgcag atcggaggtg
    11641 ttatacaaca ggttgatggt caacggtttg tctgcggtat aacccgcttc agccagcagt
    11701 tttttcgctt cttcgttacg tttttcctgg ctccagccaa accattccgg ctgagtcaat
    11761 tttgcgccat cagtatacgg tggagtgtaa ccataggcgg gcatgtcgcc ctgcgctttc
    11821 actttattaa caatgatatc gcggtccata cctagtttca gtgcggtacg cacacgcaca
    11881 tcgttgaatg gcggtttctg gttgttaatt tcgtaatagt aagtgcacag gtatggatca
    11941 acgtgaactt cgtccgggat ctcttttttc agcttctgga acaattcgat cggcatgttg
    12001 ttataagtca tgtcgatttc accactacgg tagcggttga catcggtaac ttcagaagca
    12061 ataggcaaat aggttacctg gttaataacg gttttcgcgt tgttccagta ggtcgggctg
    12121 cgttcaagaa cgattcgttc gtttacgacc caatctttta aggtataggc accgttggtg
    12181 acgatattac caggctgggt ccatttttcg ccgaatttct cgatagcggc ttttggcacc
    12241 ggtgaagttg atgggtgaac aagtaattta tagaagtacg gaacgggttc acttaaggtg
    12301 acttctaatg tgtgatcatc aatagctttc acgccgagat cggtaatcgg ttttttccct
    12361 tcaagaattt catcaatacc ggcgatatgc ccatattgca gataactggc atacggagaa
    12421 gcagtgttcg gatcaacaga acgttgccag ctatacacaa agtcttgtgc tgtgactggc
    12481 gtgccatcag accatttcgc atctttacgc aaatggaagg tccagacttt cgcgtcttta
    12541 ttatcccagg attcagcgac gccaggtgct ggatgaccgt taagatcgct gaccagtaag
    12601 ccttcaaaca ggtctcggct gatattagac tccggaacac cttcaatttt gtgcggatct
    12661 aatgactgaa cttctgaacc attgttacgt accagtgttt gtttttccgc cagtgtgacg
    12721 cctgcgggta catcagctgc cagcgcgaca ttccctgcca ttagcgcagc cagaacgcca
    12781 gctgctacta aacttctctt ggtgatgttg gtcattgttt tttggactcc ctcattataa
    12841 ttactggtat taaccagcat gtgtaatccc ggattgggac cctgcacagg ctcggagact
    12901 ttctgctgtc aggttacttt atcgttacgt attactatca ccgactttat ttattctggt
    12961 gattcagacg atcaccttat gtcgattcgt actgtgtctc ccctgtcgag acacattatt
    13021 ctttcgcgac gctctttaat tgttaatta
//
LOCUS       DAZGTR010000117        13009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGTR010000117 DAZGTR010000000
VERSION     DAZGTR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.119"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(64..1314)
                     /gene="icd"
                     /locus_tag="ACHLCY_003814"
     CDS             complement(64..1314)
                     /gene="icd"
                     /locus_tag="ACHLCY_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP0370986.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1486..2139
                     /gene="rluE"
                     /locus_tag="ACHLCY_003815"
     CDS             1486..2139
                     /gene="rluE"
                     /locus_tag="ACHLCY_003815"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP0370987.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2149..2610
                     /gene="nudJ"
                     /locus_tag="ACHLCY_003816"
     CDS             2149..2610
                     /gene="nudJ"
                     /locus_tag="ACHLCY_003816"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP0370988.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2664..3770
                     /gene="mnmA"
                     /locus_tag="ACHLCY_003817"
                     /gene_synonym="trmU"
     CDS             2664..3770
                     /gene="mnmA"
                     /locus_tag="ACHLCY_003817"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP0370989.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3806..4447
                     /gene="hflD"
                     /locus_tag="ACHLCY_003818"
     CDS             3806..4447
                     /gene="hflD"
                     /locus_tag="ACHLCY_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP0370990.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4451..5821
                     /gene="purB"
                     /locus_tag="ACHLCY_003819"
     CDS             4451..5821
                     /gene="purB"
                     /locus_tag="ACHLCY_003819"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP0370991.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5989..6660
                     /gene="phoP"
                     /locus_tag="ACHLCY_003820"
     CDS             5989..6660
                     /gene="phoP"
                     /locus_tag="ACHLCY_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP0370992.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6660..8120
                     /gene="phoQ"
                     /locus_tag="ACHLCY_003821"
     CDS             6660..8120
                     /gene="phoQ"
                     /locus_tag="ACHLCY_003821"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP0370993.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8196..9317
                     /gene="roxA"
                     /locus_tag="ACHLCY_003822"
                     /gene_synonym="ycfD"
     CDS             8196..9317
                     /gene="roxA"
                     /locus_tag="ACHLCY_003822"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP0370994.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9463..10692)
                     /gene="pepT"
                     /locus_tag="ACHLCY_003823"
     CDS             complement(9463..10692)
                     /gene="pepT"
                     /locus_tag="ACHLCY_003823"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP0370995.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMREIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            10942..12078
                     /gene="potA"
                     /locus_tag="ACHLCY_003824"
     CDS             10942..12078
                     /gene="potA"
                     /locus_tag="ACHLCY_003824"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP0370996.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12062..12925
                     /gene="potB"
                     /locus_tag="ACHLCY_003825"
     CDS             12062..12925
                     /gene="potB"
                     /locus_tag="ACHLCY_003825"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP0370997.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDN"
BASE COUNT         3173 a         3321 c         3287 g         3228 t
ORIGIN      
        1 acctacataa ataaaacaac gggcgtgtta tacgcccgtt gcaatattaa acacatgtag
       61 tgattacatg ttcttgatga tcgcatcacc aaattctgaa catttcagca gtttagcgcc
      121 ttccatcaga cgctcgaagt cataggttac ggtcttcgca ttgattgcgc cttccatacc
      181 tttaacaatc aggtctgcgg cttcagtcca gcccatatgg cgtaacatca tctcagcgga
      241 gagaataata gagccagggt ttactttgtc ctgaccggcg tatttcggcg cagtaccgtg
      301 ggtggcttca aacagggcgc attcgtcacc gatgtttgct ccaggggcga taccgatacc
      361 gccaacctgc gctgccagag cgtcagaaat gtagtcaccg ttcaggttca tacaggcgat
      421 aacatcatat tcagccggac gcagcaggat ctgttgcagg aatgcatcag caatcacgtc
      481 tttaatgacg atctctttgc cggtgttcgg gtttttaact ttcagccacg ggccgccgtc
      541 gatcagttca ccgccaaact cttcacgcgc cagctggtag ccccagtctt taaacgcgcc
      601 ttcggtgaac ttcatgatgt tgcctttgtg caccagagtc acagagtcac gatcgttagc
      661 aattgcgtat tcgatcgctg cacgaaccag acgtttggtg ccttcttccg aacacggctt
      721 aataccgata ccacaatgtt ccgggaagcg aattttcttc acccccatct cttcacgcag
      781 gaatttaatc actttctcgg cgtcggcaga gtctgctttc cattcgatac ccgcataaat
      841 gtcttccgag ttttcacgga agataaccat atcggtcagt tcagggtgtt taaccgggct
      901 tggagtgcct tgatagtaac gtaccggacg caggcagatg tagagatcca gttcctggcg
      961 cagggcaacg ttcagagagc gaataccgcc accaacagga gtggtcagcg gacctttaat
     1021 ggcaacgcga tattcacgaa tcagatcaag agtttcagca ggcagccaga cgtcctgacc
     1081 ataaacctgt gtggattttt caccggtgta aatttccatc caggagattt tacgctcgcc
     1141 tttataggct ttctcgactg cagcgtcgac cactttcagc atggctgggg ttacatctac
     1201 accgattcca tcaccttcaa tgtaagggat aatcggattt tcaggaacgt tgagtttgcc
     1261 gttttgcagg gtgatcttct tgccttgtgc cggaacaact actttacttt ccattcacct
     1321 ctccttcgag cgctactggt ttgctcgtct gccaccacgt tgcatatgcg tttgcgtcct
     1381 gcgatacgga tgctttagag caattttttg ttaatgattt gtaattggct tgtcaatact
     1441 accctattgt ttgccgtcat gaaagatgcg cgttattagg ctataatgcg gcaattcata
     1501 atctctgaaa ataccatgca aaaaacttct tttagaaatc accaggttaa gcgattcagc
     1561 tcgcaacgtt ctaccaggcg taaacctgaa aaccagccca cgcgtgtgat cctgttcaat
     1621 aaaccctacg atgttcttcc gcagttcacc gatgaagccg gacgcaaaac attaaaagaa
     1681 ttcatcccgg ttcagggcgt ttatgcagca ggtcgtcttg accgcgatag cgaagggtta
     1741 ctggtgctga ccaataacgg cgcgttgcag gcgcgtttaa cccagccggg caaacgcacc
     1801 gggaaaatct attatgtgca ggtggaaggt attcccacac aagacgcact tgaagccttg
     1861 cgcaatggcg taaccttaaa tgatggccct accctgcccg caggcgcgga gctggttgac
     1921 gaacccgcgt ggttatggcc gcggaatcca ccaattcgtg aacgcaaaag tattcccacc
     1981 agctggctga agatcacctt atatgaagga cgtaatcgcc aggtgcgccg catgaccgcc
     2041 catgttggtt tccccacgct gcgactgatt cgctatgcga tgggagatta ctctttggat
     2101 aatcttgcca atggcgaatg gcgagaagtg acagattaag gatgcaaaat gtttaaaccg
     2161 cacgttaccg ttgcttgcgt ggtgcacgca gaaggcaaat ttttagtcgt tgaagagacg
     2221 attaatggta aagcgttatg gaaccaacct gccgggcatc tggaagccga tgaaacctta
     2281 gtggaagccg ccgcccgtga actgtgggaa gaaaccggca tcagcgcgca gccgcaacac
     2341 tttattcgta tgcatcagtg gattgcgcca gataaaacgc cgtttttgcg tttcctcttt
     2401 gccattgagc ttgagcaaat atgcccgacc cagcctcatg acagcgatat cgactgctgt
     2461 cgttgggtca gcgccgaaga aattttacag gcgtcaaatc ttcgttcgcc gctggtggcg
     2521 gaaagtatcc gttgttatca aagcgggcaa cgctatccgc tggagatgat tggcgatttt
     2581 aactggcctt ttacaaaggg tgtcatctaa aaggctgcgt gatagaatac gccgccttga
     2641 agttcaatgt cgtgagtgat ccaatgtctg aaaccgcaaa aaaagtaatc gtcggcatgt
     2701 ccggcggtgt cgattcctcc gtttctgcct ggctgttgca acaacaggga tatcaggtcg
     2761 aaggcctgtt tatgaagaac tgggaagaag acgacggtga ggaatattgc acagcggcag
     2821 cggatctggc tgatgcccag gctgtctgcg acaagctcgg cattgaactg cacaccgtta
     2881 actttgctgc cgagtactgg gacaacgtct tcgaactgtt ccttgccgaa tataaagccg
     2941 gtcgcacgcc gaatccggat attctgtgca acaaagagat caaatttaaa gccttcctcg
     3001 aatttgccgc cgaagattta ggtgccgatt atatcgctac cggtcattac gtacgtcggg
     3061 ccgatgtcga tggcaagagc cgcctgctgc gtggtctgga cagcaataaa gaccagagct
     3121 acttccttta tacgctcagc catgagcaga ttgcgcaaag cctgttcccg gtcggcgaac
     3181 tggaaaaacc gcaggtgcgt aagattgctg aagatcttgg tctggtcacc gcgaagaaaa
     3241 aagactctac cggcatttgc ttcattggcg aacgtaaatt ccgcgagttc ctgggccgtt
     3301 atctcccggc gcaaccgggc aaaatcatta ccgtcgatgg cgatgaaatt ggtgagcacc
     3361 aggggctgat gtatcacact ctcggtcagc gtaaaggtct gggtatcggt ggcaccaaag
     3421 aaggtaccga agaaccgtgg tatgtggtgg acaaagacgt cgaaaacaac attctggttg
     3481 tcgctcaggg ccatgaacac ccgcggctga tgtctgtcgg gttgattgcc cagcagttgc
     3541 actgggtcga tcgcgaacca ttcaccggca ctatgcgttg cacggtaaaa acccgctatc
     3601 gccagaccga tatcccttgc accgtcaagg cgctggacga tgatcgcatt gaagtgattt
     3661 tcgatgaacc ggttgccgcc gtgacgccgg gccagtctgc cgtcttctat aacggtgaag
     3721 tgtgcctcgg tggcggtatt attgagcagc gtctgccgct gccggtctga ttattatctt
     3781 tacttaaaac aaggaagcag tgaacgtggc aaagaattac tatgacatca ccctcgccct
     3841 ggccggtatt tgtcagtcgg cacgcctggt gcaacaactc gctcaccagg ggcattgtga
     3901 tgccgatgcg ctacacgtct cactcaacag tattattgat atgaacccca gctcgacgct
     3961 ggcggttttt ggcggtagcg aagccaacct gcgcgtcggg ctggaaaccc tgctcggcgt
     4021 gctcaatgcc agcagtcgcc agggcttaaa cgccgaatta acccgctaca cactcagctt
     4081 gatggtgctt gagcgcaaac tctcctcagc gaaaggcgcg ctcgacactc tgggcaaccg
     4141 gatcaacggc ctgcaacgcc agctcgaaca cttcgattta cagtccgaaa cgctgatgag
     4201 cgcgatggct gctatctatg ttgatgtgat cagcccgctt ggcccgcgca ttcaggtcac
     4261 cggttcccct gctgtactgc aaagcccaca agtgcaggcg aaagttcgcg caaccctgct
     4321 ggcaggcatt cgcgccgccg tgctctggca ccaggtcggc ggcggacgtc tgcaactgat
     4381 gttttctcgt aatcgcctga ccactcaggc aaaacaaatt cttgctcatt taaccccgga
     4441 gttgtgatct atggaattat cctcactgac cgccgtttcc cctgtcgatg gacgctacgg
     4501 cgataaagtc agcgcgctgc gcgggatttt cagcgaatat ggtttgctga aattccgtgt
     4561 acaagttgaa gtacgttggc tgcaaaaact ggccgcgcac gcagcgatca aggaagttcc
     4621 tgcttttgct gccgacgcaa tcggttacct tgatgcaatt gtcgccaatt tcagcgaaga
     4681 agatgccgca cgcatcaaaa ccatcgagcg taccactaac cacgacgtta aagcggttga
     4741 gtatttcctg aaagaaaaag tggcggagat cccggaactg cacgcggttt ctgaattcat
     4801 ccactttgcc tgtacttcgg aagatatcaa taacctctcc cacgcattaa tgctgaaaac
     4861 cgcgcgtgat gaagtgatcc tgccgtactg gcgtcaactg attgatggca ttaaagatct
     4921 cgccgttcag taccgcgata tcccgctgct gtctcgtacc cacggtcagc cagccacgcc
     4981 gtcaaccatc ggtaaagaga tggcaaacgt cgcctaccgt atggagcgcc agtaccgcca
     5041 gcttaatcag gtggagatcc tcggcaaaat caacggcgcg gtcggtaact ataacgccca
     5101 tatcgccgct tacccggaag ttgactggca tcagttcagc gaagagttcg tcacctcgct
     5161 gggtattcag tggaacccgt acaccaccca gatcgaaccg cacgactaca ttgccgaatt
     5221 gtttgattgc gttgcgcgct tcaacaccat tctgatcgac tttgaccgtg acgtctgggg
     5281 ttatatcgcc cttaaccact tcaaacagaa aaccattgct ggtgagattg gttcttccac
     5341 catgccgcat aaagttaacc cgattgactt cgaaaactcc gaagggaatc tgggcctttc
     5401 caacgcggta ttgcagcatc tggcaagcaa actgccggtt tcccgctggc agcgtgacct
     5461 gaccgactcc accgtgctgc gtaacctcgg cgtgggtatc ggttatgcgc tgattgcgta
     5521 tcaatccacc ctgaaaggcg tgagcaaact ggaagtaaac cgtgaccatc tgctggatga
     5581 gctggatcac aactgggaag tgctggctga gccaatccag acagttatgc gtcgctatgg
     5641 catcgaaaaa ccgtacgaga agctgaaaga gctgactcgc ggtaagcgcg ttgacgccga
     5701 aggcatgaag cagtttatcg atggtctggc gttgccagaa gaagagaaag cccgcctgaa
     5761 agcgatgacg ccggctaact atattggtcg agctatcacc atggttgatg agctgaaata
     5821 aactcgtatc agtgccggat ggcgatgctg tccggcctgt ttattaaaat tatccacttt
     5881 ttcttttttc actttacctc ccctccccgc tggtttattt aatgtttacc cccataaaca
     5941 cataatcgcg ttacactatt ttaataatta agacagggag aaataaaaat gcgcgtactg
     6001 gttgttgaag acaatgcgtt gttacgtcac caccttaaag ttcagattca ggatgctggt
     6061 catcaggtcg atgacgcaga agatgccaaa gaagccgatt attatctcaa tgaacattta
     6121 ccggatattg cgattgtcga tctcggattg cctgacgagg acggtctgtc actgattcgc
     6181 cgctggcgta gcaatgatgt ttcactgccg attctggtat taaccgcccg tgaaagctgg
     6241 caggacaaag tcgaagtatt aagtgccggt gctgatgatt acgtgactaa accgtttcat
     6301 attgaagagg tgatggcgcg aatgcaggca ttaatgcggc gtaatagcgg cctggcttca
     6361 caggtcattt cgctaccccc gtttcaggtt gatctctctc gccgtgaatt atctattaat
     6421 gacgaagtga tcaaactgac cgcgttcgaa tacaccatta tggaaacgtt gatacgcaat
     6481 aatggcaaag tggtgagcaa agattcgtta atgctccaac tctatccgga tgcggagctg
     6541 cgggaaagcc ataccattga tgtactgatg ggacgtctgc gcaaaaaaat tcaggcacaa
     6601 tatccgcaag aagtgattac taccgttcgc ggccagggct atttgttcga attgcgctga
     6661 tgaaaaaatt actgcgtctt tttttcccgc tctcactgcg ggtacgtttt ctgttggcaa
     6721 cggctgcggt agtactggtg ctttcgcttg cctacggaat ggtcgcgctg atcggttata
     6781 gcgtcagttt cgataaaact acgtttcggc tgttacgtgg cgagagcaat ctgttctata
     6841 cccttgcgaa gtgggaaaac aataagttgc atgtcgagtt acccgaaaat atcgacaagc
     6901 aaagccccac catgacgcta atttatgatg agaacgggca gcttttatgg gcgcaacgtg
     6961 acgtgccctg gctgatgaag atgatccagc ctgactggct gaaatcgaat ggttttcatg
     7021 aaattgaagc ggatgttaac gataccagcc tcttgctgag tggagatcat tcgatacagc
     7081 aacagttgca ggaagtgcgg gaagatgatg acgacgcgga gatgacccac tcggtggcag
     7141 taaacgtcta cccggcaaca tcgcggatgc caaaattaac cattgtggtg gtggatacca
     7201 ttccggtgga gctaaaaagt tcctatatgg tctggagctg gtttatctat gtgctctcag
     7261 ccaatctgct gttagtgatc ccgctgctgt gggtcgccgc ctggtggagt ttacgcccca
     7321 tcgaagccct ggcaaaagaa gtccgcgaac tggaagaaca taaccgcgaa ttgctcaatc
     7381 cagccacaac gcgagaactg accagtctgg tacgaaacct gaaccgattg ttaaaaagtg
     7441 aacgcgaacg ttacgacaaa taccgtacga cgctcaccga cctgacccat agtctgaaaa
     7501 cgccactggc ggtgctgcaa agtacgctgc gttctctgcg tagtgaaaag atgagcgtca
     7561 gtgatgctga gccggtaatg ctggagcaaa tcagccgcat ttcacagcaa attggctact
     7621 acctgcatcg tgccagtatg cgcggcggga cattgctcag ccgcgagctg catccggtcg
     7681 ccccactgct ggacaatctc acctcagcgc tgaacaaagt gtatcaacgc aaaggggtca
     7741 atatctctct cgatatttcg ccagagatca gctttgtcgg tgagcagaac gattttgtcg
     7801 aggtgatggg caacgtgctg gataatgcct gtaaatattg cctcgagttt gtcgaaattt
     7861 ctgcaaggca aaccgacgag catctctata ttgtggtcga ggatgatggc cccggtattc
     7921 cattaagcaa gcgagaggtc attttcgacc gtggtcaacg ggttgatact ttacgccctg
     7981 ggcaaggtgt agggctggcg gtagcccgcg aaatcaccga gcaatatgag ggtaaaatcg
     8041 tcgccggaga gagcatgctg ggcggtgcgc ggatggaggt gatttttggt cgccagcatt
     8101 ctgcgccgaa agatgaataa atatgtccat acttcacgca ttacgttaag catccgttat
     8161 aatcggttgc agataccagc ctgtggatgc ttaacatgga ataccaactc actcttaact
     8221 ggcccgattt tcttgaacgt cactggcaga aacgcccggt ggtgttaaaa cgcggcttta
     8281 ataattttat tgacccgatc tctccagacg agttggcggg tctggcgatg gaaagcgaag
     8341 ttgacagtcg actggtcagt caccaggatg gcaaatggca ggtcagccac ggcccgttcg
     8401 aaagctacga tcatctcggt gaaaccaact ggtcattact ggtacaggca gtgaaccact
     8461 ggcatgagcc gaccgccgcg ctgatgcgac cgttccgtga actaccggac tggcgtattg
     8521 atgatctgat gatctctttt tctgtaccgg gcggcggcgt cggtccgcat ctcgatcagt
     8581 acgacgtgtt tatcattcag ggtaccggtc gtcgtcgctg gcgagtgggc gaaaagctgc
     8641 aaatgaaaca gcactgtccg cacccggatc tgttacaggt cgatccgttc gaagccatca
     8701 tcgatgaaga gctggagcct ggcgatattc tttatattcc gccaggattc ccgcatgaag
     8761 gctacgcgct ggaaaatgcg atgaactatt ccgtgggttt tcgcgcgcca aatacgcggg
     8821 aattaattag cggatttgcc gattatgtgc tgcaacgtga actgggcggc aactactaca
     8881 gcgatccgga tgttccacct cgcgctcatc ctgcggacgt tctgccgcaa gagatggata
     8941 aactgcgtga gatgatgctc gaattgatca accagccgga acactttaag caatggtttg
     9001 gcgagtttat atcccagtca cgtcatgaac tggatatcgc gccgccggaa ccgccttatc
     9061 agccagatga aatctacgat gcgctgaaac aaggtgaagt gctggtgcgc ctgggtggtc
     9121 tgcgcgtatt gcgcattggc gacgacgtgt atgccaatgg tgagaagatc gattccccgc
     9181 accgtccggc actggatgca ctcgccagca acattgcgct gactgcggag aattttggcg
     9241 atgcgctgga agatccgtca ttcctcgcga tgctcgcggc gctggtcaat agcgggtatt
     9301 ggttcttcga agggtaagtt tggatttaag ccggatgcgg cgctgattgc gccttatcgt
     9361 gcctacatgc actgaacgcc tgtaggcacg ataagtatgt ggaccagtat caatataacg
     9421 gtttcatccc ggtaattggc agtccacata cgcatcgggc aatcacgact tccgttgcgc
     9481 cgttaactcg gcaatacgga caatcacctg caccgctttt tccatacctt ccagagtcac
     9541 aaactcatgc ttaccatgat agttgtaacc gccagtgaac aggttcgggc acggtaatcc
     9601 cataaacgac aactgcgcgc cgtcggtacc gccgcggatc ggtttcagtt ccggttcaat
     9661 atcgcagtca cgcatcgcct gctgggcgat atcgataata tgcggatgct caaccacttt
     9721 ctcgcgcata ttgtagtaac tgtcttcaat caccagttca atgtagcaat caggatgtaa
     9781 ccctttgccc acttttttgg cgatctccct cattttacgt ttacgcgctt caaactgttt
     9841 acggtcgaaa tcacggatga tgtagtgcat atcggctcgg tcaacggtgc ctttcatgct
     9901 cgccaggtga tagaaacctt catagccttc tgtcatttcc gggctttcat ccgccggaac
     9961 ttccgcatga atacgtgccg ccagcgacag cgcatttacc atcactcctt tcgccgtgcc
    10021 cggatgaaca ttgttaccga caattttgat attgaccgat gcggcgttga agttttcaaa
    10081 ctccagttcg cctacgccac caccgtcaac agtgtaagcc cagcgggcat cgaaggcatc
    10141 aacatcaaaa tgtttcgccc ctttgcccac ttcttcatcc ggggtaaagg cgacgcgaat
    10201 atcaccatgc ggaatgtttt tctgttgcaa taccgccagc gcggtcatga tttctgcaat
    10261 acctgctttg tcatcggcac ctaacaaggt tttaccatcg gtggtaatca acgtctgacc
    10321 cagtagctga tgcagcaccg ggaacataac cggtgataaa acttcatcgc cgatacccag
    10381 cgcaatatcg ccaccgcgat agttttcaac aatttgcgga ttcacatttt tgccgctgca
    10441 atccggtgag gtatccacat gagaaataaa gccaatcgcc gggatatcgc cagggacgtt
    10501 agccggtaac gtcgccatca aagtgccctt ctcacttaag gtcacattga taagccccat
    10561 ctcttcgagc tgctctttca gcagatgcaa taacttccat tggccttccg tgctgggaac
    10621 ctgtctcacc cctgcttttg attgggtatc cagagacacg tagttcaaaa atcgctcaag
    10681 tagtttatcc atgtagtcac cctcactttt tgtgacaaca ttattaagaa gcaagaaaag
    10741 acaaatattg cgtcaggtca cttttacccc tgcaagcggg aatatttatc agcattacct
    10801 taatgaatat aaagctaagc cagtaattca caacaaagat tggcgattca agcggtttgc
    10861 cgtagaatta ccgccctcat taagagtcac caaggtggtt aaccacaaac cccgcatcgg
    10921 taagccatcc gttgcgttta catgggacag agtaaaaaat tgaataaaca accgagttcg
    10981 ctttcaccgc tggtgcaatt ggcgggaatt cgcaaatgct ttgatggtaa agaggtcatt
    11041 ccccagctgg atctgactat caacaatggc gagttcctca cgctgcttgg cccttctggc
    11101 tgcggtaaaa caaccgttct tcgcctgatt gcaggtctgg aaactgttga ttccggacgc
    11161 atcatgctgg ataacgagga catcacccac gttccggcgg aaaaccgcta tgtgaacact
    11221 gttttccaaa gctacgcact tttcccccac atgaccgtgt tcgaaaatgt ggcctttggg
    11281 ttgcgcatgc aaaaaacccc cgctgctgaa attacgcccc gcgtgatgga agccctgcgg
    11341 atggtgcaac tggaaacctt cgctcaacgt aaaccgcatc agctctctgg tggtcaacag
    11401 caacgcgtcg ccattgctcg cgcggtggtt aacaagcctc gtctgttgtt gctggatgag
    11461 tcgctctcag cgctggatta caaactgcgt aagcaaatgc agaacgagct gaaagcgtta
    11521 cagcgtaagc ttggtattac cttcgtcttt gtcactcacg atcaggaaga agcgctcacc
    11581 atgtcagaca ggattgtggt gatgcgcgag ggtcgtattg agcaagatgg tacaccgcgc
    11641 gaaatttacg aagagccgaa aaacctgttt gttgccggct tcattggcga aatcaatatg
    11701 tttaacgcca ctgtcatcga acgtctagac gagcagcgcg tacgcgccaa cgttgaaggc
    11761 cgcgaatgta atatctacgt taacttcgcc gttgaaccgg ggcaaaaact gcatgttctg
    11821 ctgcgcccgg aagacttacg tgttgaagag attaacgacg acaaccacgc tgaagggctg
    11881 attggttacg ttcgtgagcg taactacaaa ggcatgacgc tggagtcggt tgttgaactg
    11941 gaaaatggca agatggtgat ggtcagcgaa ttcttcaatg aagacgatcc tgactttgac
    12001 cactctctcg accaaaaaat ggccattaat tgggtagaaa gctgggaggt cgtactggct
    12061 gatgaagaac acaagtaagt tccagaatgt agtgattgtc actattgtcg gttggcttgt
    12121 gttgtttgtc tttctgccca acctgatgat cattggcacc agctttttga cccgcgacga
    12181 cgccagtttc gtaaaaatgg tctttacgct ggataactac acgcgtctgc tcgatccgct
    12241 ctattttgaa gtgctattgc actcgctgaa tatggcgctg atcgccaccc tcgcctgcct
    12301 ggtgctgggc tacccgtttg cctggtttct ggcgaagctg ccacacaagg tgcgtccgct
    12361 gctgctgttt ctgctgattg ttcccttctg gaccaactca ttgattcgta tctacgggct
    12421 gaaaattttc ctcagcacca aaggctatct caacgagttt ttgctctggc tgggcgttat
    12481 cgacacacca atccgtatca tgttcacgcc cagtgcggtg attatcggtc tggtttacat
    12541 tctgctgccg tttatggtga tgccgctgta ctccagtatc gaaaaactgg ataagccgtt
    12601 gcttgaggcg gcgcgcgatc tcggtgccag caagttacag acttttatcc gtatcattat
    12661 tccactgacg atgccgggaa ttattgccgg atgtctgctg gtgatgctgc cagcgatggg
    12721 cctgttctat gtatccgacc tgatgggcgg tgcgaaaaac ctgctgatcg gtaacgtcat
    12781 caaggtacag ttcctcaata ttcgtgactg gccatttggt gcagccacca gcattacgct
    12841 gactatcgta atgggcctga tgttgctggt ttactggcgc gcttctcgtc tgctgaataa
    12901 gaaggcaaca gaaatagata attagtgctt tatattcaaa aggttaatag gaaatttggc
    12961 atgcttttta ctgtctagta aacagcatgc ctctatccat aaaatcaaa
//
LOCUS       DAZGTR010000118        12994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGTR010000118 DAZGTR010000000
VERSION     DAZGTR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.120"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(57..617)
                     /locus_tag="ACHLCY_003826"
     CDS             complement(57..617)
                     /locus_tag="ACHLCY_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP0370998.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(720..1580)
                     /locus_tag="ACHLCY_003827"
     CDS             complement(720..1580)
                     /locus_tag="ACHLCY_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0370999.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(1639..2385)
                     /gene="traX"
                     /locus_tag="ACHLCY_003828"
     CDS             complement(1639..2385)
                     /gene="traX"
                     /locus_tag="ACHLCY_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP0371000.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGSSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(2405..7675)
                     /gene="traI"
                     /locus_tag="ACHLCY_003829"
     CDS             complement(2405..7675)
                     /gene="traI"
                     /locus_tag="ACHLCY_003829"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFP0371001.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSTFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFTELGREIDAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLSASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIVGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQEGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            complement(7675..9828)
                     /gene="traD"
                     /locus_tag="ACHLCY_003830"
     CDS             complement(7675..9828)
                     /gene="traD"
                     /locus_tag="ACHLCY_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0371002.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(10081..10812)
                     /gene="traT"
                     /locus_tag="ACHLCY_003831"
     CDS             complement(10081..10812)
                     /gene="traT"
                     /locus_tag="ACHLCY_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP0371003.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(10826..11335)
                     /gene="traS"
                     /locus_tag="ACHLCY_003832"
     CDS             complement(10826..11335)
                     /gene="traS"
                     /locus_tag="ACHLCY_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFP0371004.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            complement(11332..>12994)
                     /locus_tag="ACHLCY_003833"
     CDS             complement(11332..>12994)
                     /locus_tag="ACHLCY_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HFP0371005.1"
                     /translation="GAPVVLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAG
                     FSSVYSHFASSAISPTASAAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQT
                     GSGATATQTRDGNMVMDASGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFS
                     SSIASAWNTLSQFGSNRGSSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNE
                     QATQELASTSTRLSGGMYADAHAEWGVKPKILGVGGGVGIRGGFKGSLDATDDDSHSA
                     SSGSRATHDARHDIDAKASKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNS
                     AKQSYDQYTTNMTRSHEYAEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTN
                     TSSPEIAERRRAMAWSFVQEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQ
                     GHQAIIDQRTQDSNIRNDVKHQVDNMVTEYKGNIGDTQNSIHGEENIVDRQYSELKSN
                     HKQEEIQQNNRYNEENKRQKLMPTPSEDALKQMVDDKKERLKGPL"
BASE COUNT         2822 a         3932 c         3025 g         3215 t
ORIGIN      
        1 gaaggtttca gagaagcctt atttttccgc ctcctttagt gtgaaggagg ctttttttat
       61 tgttcatcaa gcacggcctg aagttctgcc tttatccggt tctggcggcg gattttatcc
      121 agacgctctg ccgcatacac ttcttcctcc tgagaaatat gctccgtcac atacccttcc
      181 gtgtcatacc ggcaggcacc ggctttcatg gcacacagat agctttctga acgggtgatg
      241 gccttcagcg ccctgcgcag ttttttatgc gagagcggga tattccgctg cgccacgtct
      301 tccagtaaca cgtcccggat accgcaggcc agaagccggg gtgtgtcacc gtcaaataat
      361 cccggccacc agggtttcag ggtgttcacg gcctcatcca gcgagggcag gttcagataa
      421 atggacagcg cctgtctggc ctgcgctttt tttgctgtca gctctgcttc ccgggcagcc
      481 ttctccgcca gtttctgctt tttcaccttc cattttggtg gcgtggtgac attgatgatg
      541 gttttccggc tccgggtcgg tgtttcccct tccgttttcc gtttcagtgt cagtaccggt
      601 cgtttctgct ctgtcatgat tgccctgacc tttgtgaaaa tatgtcctcc aggtcgttga
      661 aactactcac tatgatatta agctgacctc aacgacctct gacatattaa taccgacaat
      721 taaagatgtt ttttaaacca ttgcgtctgt actgccgcca cacgttcaaa aaacgcgcct
      781 tcataaatat cataatggca tgcgtcagct tcctcgtata attccttagt accagaagca
      841 acagcatcat ataaggctct tccctgttct ggtggattaa cactatcctg gccggcaata
      901 acaaccaaaa ctgggcactg tactttggct gcgctctctg ccggtttata ttgcaacgtc
      961 tccataaccg taaggaaagg tatttttata tccatttcag gatactgacc ttttaccttc
     1021 tcgaagaata cctttgattc attatcactc agtacccggg taacccccac aaacatctct
     1081 ttcccggtat ttttcttctt ttctgccatt ttattcagcg tggacagaaa agaagctttc
     1141 tccagttcat tcatttctcc ggtaaccaga acctcaccgt cagcaaaagc cagctggctg
     1201 acaatacttt tcactctttt atcctgcgca gctgcattga atacatggca cccacccagc
     1261 gatgttcccc ataatcctat acgctgatta tcaatacata cctgcttttc tgcccaattg
     1321 ataactgaaa taatatcttc ggtctgcatt gccggaacca gacgacctcg ttccccttca
     1381 ctctcaccga atccacgata atcaaaagta atcgttgcaa acccggcttc tgtaaacgca
     1441 ttcgcaaagg aaggcagcag aacattccgg ataccgcaaa atccatggca taagagaatc
     1501 aaaggatgtt ttatatttcc ttccgggaca cgaaatgtca gggcaatacc ttcagagagt
     1561 ttgtgatccg taattttcat aaatatcctg tgttatgttg tttattaata atcagaatca
     1621 gcgtcagtat cataccagtc acaacgccag aacgcccagc acagccagat gacaggcgta
     1681 gaataccggg aaaaaatccc cgggccagaa acggggtaat gattttcctg cacacgacac
     1741 cagaccaacg gtcagcaccg tcatcacaag accggccacc gccgctgcat cactggtggc
     1801 aaggttaagc gctggaataa cagccagcag gcaggcgacc agcgccagac gttccgctct
     1861 gtcttccgcc cggtacagcc ggtgactgac tgccagcatc agcagcccgg caatgccgta
     1921 actggagccg gacaaaggcc cccacagggc catcagaaga atggccgctg ctgtacgcca
     1981 cccactgcgc gtttcacacc acgtcagcac ctgtgccgcc actgcaaagg caaacaggat
     2041 attcccctca taccagggaa agccggccag ataatatgcg aactgggcaa taattcccca
     2101 tccccacagc cggttaatgg ccggttgccg gatatgcgca tgacgggaca gattcagccc
     2161 ccacaccagg gcaaacagcg gaaaggctcc tcgcccggcc agaaacatcc attcctgctt
     2221 cagctggaat atcagattga tgtggtccag taccatcagc accagtgcca ccgttttgat
     2281 gatatcccgc tgtccgggtg accagaccag aaacgactgc aaccaggtat cggtccgtgc
     2341 agccagtgaa tcgttacgtg tcgtattcgt gttatccgtt gtcatcagtg tttgtcttcc
     2401 tgtatcagtc tccgcccagg gttttctctt tctgcaggtc acggaccata tcccgctcca
     2461 tctgctgcag acgctcctgc agcagatttt cccgggcaac ctcccggacc gcttcccgct
     2521 cccgttgtga ttcacgcagc acactctccg gcagcgcggc ttcccgttca gttatggcag
     2581 cccggtcacg ctcctgcccg gcaatctccc tgacagcctg ctctgttctg ccatccggca
     2641 ggtcaggttt gttctctgcc attttacgga caatttcatc tgcgcgacgt tccgtttcac
     2701 ggcggatggc ctcttcagcc tgccgctgtg ccagcacttc agctgtaacc ggttcaccat
     2761 ttccggcccc cggctggacg ctgttgtccg ggatatcccc ccacacgcga ccaccggtga
     2821 tggcaccggg attccacgga cggccttcac cggcgattct caccaccaca ccgctgtcag
     2881 gattatcacg ggcaatccgg acaccctcct gcatattatc agccagcagg ctttctccgt
     2941 tacggctgcc ctgcagggcc acgaactgcg catccccgct gccttttacc cgaccttcac
     3001 cgctgaatcc ccgcagcccg tttccgtcat ccgtggtcag cgggttcagc cagataccgg
     3061 cggacctgcc gttacggtca aaagccggca gtgccacata cggttgcgga tatttacgtc
     3121 ccggagcaat aaaccgtgca ggactgtctc ccccggccag ccccgcctga cggagaacgg
     3181 cacggcctgc cgccacgtcc cgcagttccc gtgccgtact gaacagccgc tgcgcattca
     3241 tgacctcccg gtccggtttc ggctcaaaca catcgtgggc agttcctttc tgtacggcat
     3301 tgttaatggc atccgtccag ccctgacggt tatcggtgta cacctgcaca tgctgcttca
     3361 tacgcgacag ggccacgtag gctgactcaa agccggccat ctgtttccgg ttaccttccg
     3421 tgccttcaag cgcgatggca aaggtttcac tcgccccctg cgcaccgtga gcggtgatgg
     3481 cataggccag gtcaatatgt tgctctgcct gctcctggcc ggggcgaatc acccgggtct
     3541 gctgtccgtc cgacagcgtg acactgtcac cggaaactgc tgtcaccgtc cagacgctgt
     3601 tggccacata accgcgctcc cggtcactct tcgtgaagcg catccggtca ccggtcccca
     3661 cccggatggt gtccggggtg tacagtgtga cgccttcagc caccgcctcc cggggcgaaa
     3721 tcagccgcgt gttaccttcc gcatcctgca gggttatcag cccgtcatcc ttgctgatac
     3781 cgacaatccg gtgatacaca ttatccacca gggcaagtgc atccgggtta ttctcccagg
     3841 tggagagacg acgcagctcc ccgtcgcgta tattcgctgt gttcaggaca ggcaccatga
     3901 cctgctcttt tcccagctca ccggcctttt cccgtgcatc atgaatcatg ctgttcagca
     3961 cgcgccggtc ctcattcagg tgcgtgacaa tcagcgtctg ctcccgtgct tccggtgttc
     4021 tgccggtata gtcgcggaca atggcttcat acagtgtcat ggggacatcc gggaaagcct
     4081 cgcctttcag catcgccttc tgctgcgctt ctgccagttt cgcttcctgg ctgtgactga
     4141 actccgtcac ggagtgctcc ggtgcccatg cgccctccag acgtggcacc tgagacggtt
     4201 tcacactctc aagcccggac agtgcccttt ccacatcccg gttaatcagg ctgtataccg
     4261 cctcccgcag ttccggcgtc tgacgcacaa tctctttcat gatggccaca tcggcagcac
     4321 tgcgcgtctg ctggagacgg aaaggctgac cgggcgcgat ggcctgcagc tggtccgtgt
     4381 caccgctggc cacagcacga ccgccaccgg ccgcaatcag ggcgtatgcc cgtgccatgt
     4441 cggtattgcc caccattgag ctctcatcga gcagaaacag cgtgttgctg aaatccggcg
     4501 tttctccgct gcgctgctgc agctgcgtgt catgcagaaa ggacgccagc gtctgtgcat
     4561 ccacgccggc gctgcgcatc tcaccgaccg cacggtgcgt gggccccagc cccacaactc
     4621 ggggacgctc actcgccgac agcatgttca cggctgacat caccgcccgg aactgtgtgg
     4681 tcttacccac accggcataa ccctgtacca ccgtgaaacg gtcggacgtt tccagtatca
     4741 tacgggtggc ggcacgctgt cccgacgtta acgtctccat gagttcgccg ggtactctct
     4801 ccatcagcgg cgtgaccgcc tccttaccct cgagaatatg gcgaagaatg ctcttttctg
     4861 cctcatacga cgcacgggaa accagcaggt ctgtgccata gcctttagcc acatccacat
     4921 gcagtaaatc accccgtttt atctgcgcat cgatttcccg ccccagttca gtaaaactgg
     4981 ttccttctgc agcaaacgac tttgcctctg tcagcaggtc cacatggctg aaggccaggt
     5041 ttttactttc caccaccgga agggccagat gaatggcctg ctgtgccggc gtgtgaagtg
     5101 cggacttctg atgactgata gccgtttcca gcgatgtctc accggcacgc gcctttatct
     5161 gctcagaaac caccgtaaag gagggatggc gggcaagttt ttccgcagta cgggtttcat
     5221 ccagtgagga atacagccgg acatcacgac cgctgcgggc cagaccgttc agggtggcgt
     5281 tgtccattgc catctgtgtg acggaggcaa aaaccttcgc actgtcactg acggaatgcc
     5341 cgggcgtttc cacccagccg ttctccagct tcagtgccgt gaacgttgaa tcagccacag
     5401 gcagggtggc cggttcagcc cgccccggca caacaaccgt catcgcatct tcactgacgg
     5461 atgccacctg caggcggtca ccgccggaga cgcggagtcc gggaattttc cctgtcaccc
     5521 tcagtcgctc gccgtctgcc accggcattt tttccggccg gaacagcgac cagctgctgt
     5581 ccagggagga aatacgcacc acctgcgttt ccccctgcgc atcccgcagg gtcaggctgt
     5641 gactctgtgc cgtcacccgg tctatcacat agcggtcatg actgcgtgtc tccggattcc
     5701 actgctccat caccattccc gggcggtaca tatcccgcag ataacggctc cggctatcca
     5761 gccagaccgg tgacagtgcc gtcatggtca cctcctgatg tccgagcacg ccctgtgttt
     5821 tcagctcact gcgaatggcc tgtgtcagta tggcctgttc ccgtacccca ctgacctgtg
     5881 ccacgctctc ttctccggct ttcacgctgg ccgcaaaatc tccggccagc cgggcatagc
     5941 ggacattacg gtctggttca ctgatgatgg tggccggtcg ctgttctccc ccctgccagc
     6001 gatatgtgtt caccccggca tccttcatgg ccatcagtgc actgcctgta ccggttcgct
     6061 gcccgctgtc ggttatcagg acctgtacgt tatgacgtgc ggcaccgtcc agcagggtta
     6121 acgtctcttt cagggagagt ttttcgccct ggtcaacgat aacagtactg cccggcgtga
     6181 aggccatgcc ttccagcagc tgacgacgtc cggttatcag ctcaccggac aaccgttcat
     6241 cctgcttcag gttcatctgc gagcgacggt cagcagcgat aatctgcacc tcccgcccct
     6301 gctcccgggc catcatgacc agttcagcca cccgctcacg ctgcccggct gcaccgccct
     6361 gcccggacac aatggccagc gacggacggt cctgtgccag aacactgacc gcatcgctgt
     6421 aaccagccgt ccgggggaca cttttctccg gatgtacggt cacccggttc tgtttcatga
     6481 tgtcacgact gagtgcccgg acggacagct catcgagcac atgaattcct gatgtgaaca
     6541 gccccttctc acggtcgagg gggataagct gctcacgact gatggcctca tcgataccgg
     6601 cgcgtgcccg ttcaatcaca ccattttccg gcggcagtat accgaccgtt ctggccagca
     6661 cgtccgtgta cgtaaactgc acttttcgtt cacttaatcc ggcaatcgcc tgtgtcaccg
     6721 cctgctgcac atccggcccc tcctgtgaaa cagccccggg cgcctgcgtg cgggtctccg
     6781 cacgctgatc cgccgcgtca cgatatgccc ggatgtcgaa cccggtttcc ttcagcgtct
     6841 gcatccattc agccattctg acttcaggat cgacgtgctg tttggatttt cgcgtatcca
     6901 gtgccgccac atcccgggat ttcagcgagg catcttcccc gacggcctcc cggatagtct
     6961 gtgagcgtcc ggaaaaggcc tccaccggta cgcccggcat ctcccacata ccgtgcttac
     7021 ccaccacttc ggtttcatag cccagcgcct caacctgctc tttcagtttt tcccggtaga
     7081 gcctgccaaa ggcaatctga ttagcgtaca cattctcaat gaaccccgtt ttccccactt
     7141 tgtcactgct cagtgtcttc cactcaccgt tatgctgcgt gacattagcc accaccgcat
     7201 gcgtgtgtaa ctgtggttcc tgatcgcgac tggtgtcgtg gttaaacagt gccatcacca
     7261 gattaccggt cagtaccgtt tctgactgtc cgtccgtcat cacccgtgtg gaggccagcg
     7321 cctccacctg acgaacagca aagtccacgg cctggttatg tgcttcaata agacgtttgt
     7381 cccccccgag catggccatc atggagacac ttttgggggc ggagaaggtc aggtcgtagc
     7441 cgggacgatg cttattactg ccatcctgca tgcggcttag atccgctccg tccggcagcc
     7501 ttccctccag aagacgggta aaaacatcct tatcgacact gccctgcagt cccagctgtt
     7561 cagcccccct gccggcccag cgttctccca tgctgcccag tacatagtaa ttatccttgt
     7621 cggtataata gttaccggca cttccggccg atctgacctg cgcgatactc atcatcagaa
     7681 atcatctccc ggctcaacat cctccccacg ctcccggtgc acattaatgt tcacctcttc
     7741 acgacgctgc atctgctgct ggatgtccgg atgattttcc tgttgccatg cctcataagc
     7801 ggccatatcc accacttcac cggattcact gatcccgggt ggcagttgct gttccatctc
     7861 ttcttccggt ttcattttca gctcctgctc gatcccccct gccggaacat tcacacctga
     7921 atctgacttc ttatcgttga tggcaggaga caccggctgt tgcggctgtt cagcctgagt
     7981 cacgtcttct ccggaaacaa cttccggcac atccggttcg aagaggctgg ccatctgacg
     8041 accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt ccgggttgat
     8101 gtcacgcgga ataaactcag gggcaacctt cggacgtgcc tgatatttca gtgagagttt
     8161 tactgccgga tacggtccgg gcaaggtgac ataacaggtc agatccggca gagactgaat
     8221 gtcggaataa ctgaccagcg tctggcgctc catatcctta ccggtcgata ccccgtcacg
     8281 taccggatca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
     8341 ctcaccggca gcgaactctg caatcttatg gctgggagaa cggaaaaagg cacgggtgtt
     8401 catgacgtca aacagcgtgg cagccgcttt ctcaccgtag atatcttcca gctgggcata
     8461 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
     8521 caggtccggc agtttgtgta acgtgggtag ctcatcacag aaaaaccaca cacgacggtt
     8581 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
     8641 cggcttcagg gaggcatggg tgtcggcatt cgacgagata aacagccagc cgtttttctg
     8701 atcttcccgg acaccccgca tccagtcacg gatggtaaaa gactcaccgt tatgctcaat
     8761 cccctgcagg taacggatgg ctttcacata gttggtcagc acagcacgga tggaaatagc
     8821 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggttcg
     8881 cagtttttcg attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
     8941 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa
     9001 cgggtcctct tttgtgccca tcgggatcag cgtgttcgcg gtattatcaa aatccggctg
     9061 tgtcaggcac tccttccaca aatcccaggc ggcacagcgg gcatccagcg gattcagtat
     9121 cttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     9181 caccatatct ccacgctgac gggcgtagtt ggccagacga cggataactt ccgacttacc
     9241 ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     9301 gtcgccaatc cgtatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     9361 cgggttctcc gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     9421 acgccccaga atccaggaga caacaaagaa ggtaatcagg caaataacca gtgccacaac
     9481 tgtagccaga acgaatgcgg accatagctg ctctccgcac cagatcatat atttatcatg
     9541 cagtacctga gcagcgttca tccggaaggt tttgccgtaa tactggatct catataccgg
     9601 ctgggacttg attaaatccc gcatgccttc cagcgtggta caccaccagt aaatacagcc
     9661 gttcacaaac gtctgccagc ttatttttac ccataaaacc agaccaacga gtatccagaa
     9721 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     9781 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgatattccg
     9841 aagaaaaaaa gtcagtcgga ctgattatta atgatgaaaa gattaataat cgttaaattc
     9901 cggcttcgcc ggcaacactt cacggtcgca caaagaattt gcacaaccgc tcagtgtttt
     9961 catatttatt tcggaaacgc cggggagaaa taaaatttat tttatttctt tccggggttg
    10021 gacaacgagc aaagcgaggc gtcagtcagg aggccggtca gaccagcctc cggaagataa
    10081 tcagagaata tttgcgattg atttggccag ctggtcttcg agaacaggct tcgcttcttc
    10141 aaatttcagg ttaaccttgt tcgcatttga gaccacacgg gtctggtatt tatgctggtt
    10201 acctgtttca gtactggtct gaattttcgc acctgatgtg ccctgacgca gggcggcaac
    10261 attatccgtt gtcaccgttg ccttagtacg ctctgcaatc tgcacatccg tgatcatggt
    10321 atagttcaca tcttccacca tcgcatctgc agccataccc accagaccgg cagcaaggcc
    10381 cacaccgagt gtggcaccgg cagaactgga attatatccg gtgataccgg cacctaacgc
    10441 tgcaccaact gctgcgccct cataaccacg gttcagccat ccctgagact cccgcagatc
    10501 catcttatcg gccttcagca cattcgcctg aatccagtag taggctttat ccggagaagt
    10561 caccacctga tatccttttg ctttcacagc atcagcaatt ttgccctgca gcccactcat
    10621 gtctttatca gacgtgtttt tgatctgcag aaataccgtg cgttcgctgg cgggttcaag
    10681 ccagatggtc tcactcatct gagtcttcac ctcaaggtta cgcttcttga ttgctgtgct
    10741 catcgcacca caccctgaaa gggccagagt ggaactgacc agtgcaacca tcatcaattt
    10801 ttttgttttc atccaaaata tccttttaac taacagactc ttttttgaac agtcctatta
    10861 ctgaggctat agctgagaga ttataacgat ttatatctat ggtatacacc aagaaaaata
    10921 atatgaatgt aatcattagt gcaaaaacat ataagataga atgcataaac gaaaccatag
    10981 aaaaaacaat caaaaacaac aaaatatatg tcttactctt agaatacata aaatttatta
    11041 tttttgaatc ctttctgaaa ctatcaggaa tcgcaaggta taacatcatt ccatttgtaa
    11101 tagccacaaa aagcaggaac cccatcattc cactagcaca tatatcaaag ccaacatccc
    11161 tgacatcact tttaaccgat actaaacgca gaaaagttgg ccataataaa acccataaaa
    11221 acacataagt tcccggcttt atgacttgta ataatgatgg gatattaaca ccctctacag
    11281 agagtacctc ctttagtttt tgcatctcca tttttatatc atagtgagtg atcataatgg
    11341 ccccttcaat cgttctttct tatcatccac catctgtttt aacgcatctt cagacggtgt
    11401 tggcattaat ttctgtcgtt tattttcttc attatatctg ttgttttgtt gaatttcttc
    11461 ctgtttgtga ttacttttta actctgaata ttgtctgtct acaatatttt cttcgccatg
    11521 aatactattc tgagtatctc cgatattccc tttatattct gtgaccatat tatcaacctg
    11581 atgttttaca tcattacgga tattactgtc ctgcgttctt tgatcaataa tggcctgatg
    11641 cccctgatgg tcagcaataa tatcctggct gccaccaccc gaaggtacgc tctccattcc
    11701 tttaccaata tccccacggg attcacgcca ggcattatca acaccaggct gcacctgttc
    11761 ctgaacaaaa gaccatgcca tcgcccgacg gcgttctgca atctccggtg aactggtgtt
    11821 tgtcagaata gcttcggcat cctgcggtgc gtgtttcatc acatactgtg caaactgttg
    11881 cgacaaatct tcgctcatct gcccactcat gctttcagta cgggatgcca tttcagcata
    11941 ttcatggctg cgggtcatat tcgtcgtgta ctggtcgtaa ctctgtttcg ctgagttcag
    12001 ggctgcagac aactggtcca cgcgggaatc ggcattattg tcagtatggc tgccggattc
    12061 actgactttg cgactggtaa agtaatcgct ggcctcttta aagtccttac tggctttagc
    12121 atcgatatca tggcgagcat catgggtagc cctgctgcca ctacttgctg aatgtgagtc
    12181 atcatccgtt gcatccaggc tccccttaaa tccacctctg atgcctaccc caccaccaac
    12241 accaagaatt ttaggtttaa ccccccattc agcatgagca tcagcataca ttccaccaga
    12301 tagcctggta cttgttgatg ccagttcctg agtcgcctgc tcattactga tattatgcgc
    12361 cttcgcatag ctttccactg cactgcgcat acggctggcc atcatggagt cctgagcact
    12421 catcgtgctg tcagcaccac cggtgacaga atcactgctc ccccggttag aaccaaactg
    12481 actgagcgtg ttccaggcac tggcaatact gctgctgaac ccatgcaggg cactttctgc
    12541 tctgttcgat gcttcccggg ccatttcctg ttgtgccgcc gcaatctgac gcgttgcatt
    12601 gatgccgacc ggtaaccggg acatcgctcc gcttgcatcc atcaccatat taccgtcacg
    12661 ggtctgtgtg gcggttgcgc cactgccggt ctggtacatc atctgaccaa acgacgtggt
    12721 gctgttggtg ctccagctga agccgttcac gttttccgtc tgcatgttgc cgtaggagta
    12781 attaccgtca accacactgc ctgccgcact ggcggtcggg ctgatagcag aggaagcgaa
    12841 atggctgtac acactggaaa atcctgcccc gagtcccctg accatcatcc aggataatgg
    12901 cgggatcatc atggaaagat acccggcagt ggaagccaga tcggagtatt tcagctgtat
    12961 ctgtgaaatt tccgaaagca ccaccggtgc acca
//
LOCUS       DAZGTR010000119        12910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGTR010000119 DAZGTR010000000
VERSION     DAZGTR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.121"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            173..1003
                     /locus_tag="ACHLCY_003834"
     CDS             173..1003
                     /locus_tag="ACHLCY_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0371006.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            1035..1970
                     /locus_tag="ACHLCY_003835"
     CDS             1035..1970
                     /locus_tag="ACHLCY_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0371007.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2063..2305
                     /gene="yhdT"
                     /locus_tag="ACHLCY_003836"
     CDS             2063..2305
                     /gene="yhdT"
                     /locus_tag="ACHLCY_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP0371008.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2295..3746
                     /gene="panF"
                     /locus_tag="ACHLCY_003837"
     CDS             2295..3746
                     /gene="panF"
                     /locus_tag="ACHLCY_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP0371009.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3758..4639
                     /gene="prmA"
                     /locus_tag="ACHLCY_003838"
     CDS             3758..4639
                     /gene="prmA"
                     /locus_tag="ACHLCY_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP0371010.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4968..5933
                     /gene="dusB"
                     /locus_tag="ACHLCY_003839"
     CDS             4968..5933
                     /gene="dusB"
                     /locus_tag="ACHLCY_003839"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP0371011.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5959..6255
                     /gene="fis"
                     /locus_tag="ACHLCY_003840"
     CDS             5959..6255
                     /gene="fis"
                     /locus_tag="ACHLCY_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP0371012.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6341..7225
                     /gene="yhdJ"
                     /locus_tag="ACHLCY_003841"
     CDS             6341..7225
                     /gene="yhdJ"
                     /locus_tag="ACHLCY_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0371013.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7309..7488
                     /gene="yhdU"
                     /locus_tag="ACHLCY_003842"
     CDS             7309..7488
                     /gene="yhdU"
                     /locus_tag="ACHLCY_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP0371014.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(7491..8153)
                     /gene="acrS"
                     /locus_tag="ACHLCY_003843"
     CDS             complement(7491..8153)
                     /gene="acrS"
                     /locus_tag="ACHLCY_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP0371015.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            8552..9709
                     /gene="acrE"
                     /locus_tag="ACHLCY_003844"
     CDS             8552..9709
                     /gene="acrE"
                     /locus_tag="ACHLCY_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP0371016.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            9721..12825
                     /gene="acrF"
                     /locus_tag="ACHLCY_003845"
     CDS             9721..12825
                     /gene="acrF"
                     /locus_tag="ACHLCY_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFP0371017.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLEHYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         3194 a         3108 c         3267 g         3341 t
ORIGIN      
        1 aaaccaataa catctctgca taaatatgaa taaattcatt tatttctcaa tgaattaatc
       61 tatttttgtt gcgatttttg gcccatgatt ttcttttcga attgtcgata ttattgaagg
      121 ataactttca aagaaaaata caaggggcta tagtgttacg cattaaaggc caatgaagag
      181 tgttaacgtg ccgagaaaat cgtctaacct actgaaacgc agattaaaaa ttgcagaaat
      241 tgttgccgcc caaggtgaaa tcaaagttga tgacttatct gcgcaacttg gtgtctctgg
      301 cgtgacgatc cgcggcgatc tcagttatct ggagcaacag ggatatttaa agcgatcatt
      361 tggcggtgct attgcgacac cactctcctc tgaaccagaa gcgatacaac cgatcgcgcc
      421 attgtctctg gcacaacaaa tggaaatagc acgacattgc gcacggttaa ttcgcgatcg
      481 tgacacgcta tttcttggac atgggacaat ctgccgtaag atcattccat tgctcagcga
      541 agtgaaaaaa ctgcgtctga tcaccaacga tcccgaacat gcgctgctgg caaatcagtt
      601 tattgatggc gaaataatac tggctggcag tgaaatgctg cgccccgaca ccgcactggc
      661 cggaaaaccg cttgaacagg cacttcagca tttcaccatt acacatagca ttctggaaat
      721 cagccatctg gatgctgatg gtactttaaa tatcaacgtt cccagactgg ccgctgcctg
      781 gcagctctgt ttcgatcatg cccagaataa gacggttatc gttgccgccg atcctgcgcc
      841 atcaacggct gctatcgggc atttgcatca ggcagaaaat gtgatcgtca gccgcagcat
      901 taacgaacaa tatcagcaac aattgcagtc ggctgatttc gttatccttt ataccagtaa
      961 tgagtgcttt acctggtcca atcgcgaaag gcttcaggaa tgagaggggt tttgtgacaa
     1021 taactatctg cgctatgggc gaattgctgg ccgagttttt gtcccgcaac ccacatcaaa
     1081 aattcactca gcctggggag tttatcgggc catttcccag cggtgcgcca gcaatttttg
     1141 ctgctcaggt ggcaaaactg tcccatcggg ccatcttctt cggatgtgtt ggtaatgatg
     1201 attttggccg actcattata gagcgcctcc gtcatgaagg tgtcattacc gatgggatcc
     1261 atgttatgaa caatgccgtc acaggtacgg cgttcgtgag ttatcaaaat ccccagcagc
     1321 gcgatttcgt ctttaatatc cctaacagcg cctgcggctt gtttactgct gagcacattg
     1381 ataaggatct gctaaaacag tgtaaccatc tccatattgt gggctcatcg ttgttctcat
     1441 ttcgcatgat cgatgtcatg cgtaaagcaa taacgacgat caaatcggct ggcggcaccg
     1501 tttctttcga tcccaatatt cgcaaagaga tgctgagcat tcctgaaatg gcgcaggctc
     1561 tcgattattt gattgaatat acggatattt tcatccccag cgaaagcgaa ctccctttct
     1621 tcgcgcgtca caaaaatctg tcagaagaac agattgttag cgatcttctc cacggcggcg
     1681 taaaacatgt ggcgataaaa cgcgcccagc gtggggccag ctattacaag cttaaaaacg
     1741 gtacattaca cgcccagcat gttacaggcc acgatatcga aattatcgat ccaacgggtg
     1801 caggcgactg ctttggcgca acgtttatca ctcttttctt atccggtttc ccggcacata
     1861 aggcactgca atatgcaaat gccagcggcg cgctcgccgt aatgcggcaa ggtccgatgg
     1921 aagggatatc ctcactggct gacattgaag actttttgca gcagcactga acggcaattt
     1981 tctgtcagat tattttccct gcctgaagag ttgccgtaga attgcccgtt tctcgtatcc
     2041 ccccatgagg aggaaccctt ccgtggacac tcgttttgtt caggcccata aagaggcgcg
     2101 ctgggcgctg gggctgaccc ttttgtatct ggcagtttgg ttagtagccg cttacttacc
     2161 tggcgttgcc cccggtttta ccggctttcc gcgctggttt gagatggcct gcatcctgac
     2221 gccgctgctg tttattggac tgtgctgggc gatggtgaaa tttatctatc gcgatatccc
     2281 actggaggat gacgatgcag cttgaagtaa ttctaccgct ggtcgcctat ctggtggtgg
     2341 tgttcggtat ctcggtttat gcgatgcgta aacggagcac cggcaccttc cttaatgagt
     2401 atttcctcgg cagccgctct atgggcggta ttgtgctggc gatgacgctc accgcgacct
     2461 atatcagtgc cagttcgttt atcggcgggc caggagctgc ttataaatac gggctgggct
     2521 gggtattgct ggcgatgatt cagcttcctg cagtctggct ttcactcggt attctcggca
     2581 agaagtttgc gattcttgcg cgccgctaca atgcagtgac gctgaacgat atgctgtttg
     2641 cccgctacca gagtcgtctt ctggtgtggc tggcgagttt gagtttgctg gttgcgttcg
     2701 ttggtgcgat gaccgtgcag tttatcggcg gtgcgcgcct gctggaaacc gcggcgggta
     2761 ttccttatga aaccgggctg ctgatttttg gtatcagcat tgcgttatat accgcctttg
     2821 gtggctttcg cgccagcgtg ctgaacgaca ccatgcaagg gcttgtgatg ctgattggca
     2881 ccgttgtgct gcttattggc gtggtacatg ccgctggcgg cttaagcaac gcagtacaga
     2941 ccttgcaaac catcgatccg caactggtta cgccacaagg cgctgacgat attctgtcgc
     3001 ctgcctttat gacgtcgttc tgggtactgg tgtgctttgg cgtgattggc ctgccgcata
     3061 ctgcggtgcg ctgtatctct tataaagaca gcaaagcagt acaccggggg atcatcatcg
     3121 gtacgattgt agtcgcaatc ctgatgttcg gtatgcacct ggccggagcg ttaggtcgtg
     3181 cagtgatccc cgatctcacc gtaccggatc tggtgatccc gacgttaatg gtaaaagtgc
     3241 tgccaccgtt tgctgccgga atcttcctgg ctgcacctat ggctgcgatc atgtcgacga
     3301 tcaatgccca gttgctgcaa agttccgcta cgatcattaa agatctctat ctgaatatcc
     3361 gtccggatca aatgcaaaac gagacgcgtc tgaagcggat gtcggcggta attacgttag
     3421 ttctcggcgc gttgctgctg cttgccgcct ggaagccgcc agaaatgatc atctggctga
     3481 atttgctggc cttcggtggg ctggaagccg ttttcctgtg gccgctggtg ctgggtcttt
     3541 actgggaacg cgccaacgcc aaaggcgcgc taagtgcgat gatcgttggc ggcgtgctgt
     3601 atgccgtact cgcgacgctg aatattcagt acctgggctt ccaccctatt gtgccctcgt
     3661 tactactaag tttgctggct ttcctggtcg gaaaccgttt cggtacatcc gtcccgcaag
     3721 ctaccgtttt gactactgat aaataaagag ttttaccatg ccttggatcc aactgaaact
     3781 gaacaccacc ggcgcgaacg cggaagatct tagcgatgcg ctgatggaag cgggtgccgt
     3841 ttctatcact tttcaggata cccacgatac gccagtattt gagccgctgc cgggcgaaac
     3901 gcgcctgtgg ggcgacaccg atgtgattgg tctgttcgac gctgaaaccg atatgaacga
     3961 cgtggtggcg attctggaaa accatccgct gctcggcgca ggcttcgcgc ataaaatcga
     4021 acaactagaa gataaagact gggagcgcga atggatggat aatttccacc cgatgcgctt
     4081 tggtgaacga ctgtggatct gccctagctg gcgtgatgtg ccggacgaaa acgccgtcaa
     4141 cgtgatgtta gatccagggc tggcgtttgg tacgggtacc catccaacca cctctctgtg
     4201 cctgcaatgg ctcgacagcc tcgatttaac cggtaaaaca gtcatcgact ttggctgtgg
     4261 ttccggcatt ctggcgatcg cggcgctgaa actgggtgca gcaaaagcca ttggtattga
     4321 tatcgatccg caggcgattc aggccagccg cgacaacgcc gaacgtaatg gcgtttctga
     4381 ccgtctggaa ctctacttac cgaaagatca gccagaagaa atgaaagccg acgtggtggt
     4441 cgctaacatc cttgcaggcc cattacgtga actggcaccg ttaatcagcg tcctgccggt
     4501 ttcaggcggt ttgctgggcc tttccggtat tctggcaagc caggcagaga gcgtttgtga
     4561 agcttatgcc gatagcttcg cactggaccc ggtcgtggaa aaagaagagt ggtgccgtat
     4621 taccggtcgt aagaattaac cttcgcatcg ccgtagggtg acgcggggcg aagtgcgagc
     4681 aagctcacaa aaggcacgta aatttgccgg ttatttacgc aaatttgcgt gccaaaattt
     4741 tcattcataa agaaaaattg agaacttact caaatttctt tgagtgtaaa ttttagtcac
     4801 tattttctaa tatgatgatt tttatgagta attatcgcac cacgctcatt ttaaatgcaa
     4861 ttctttgatc catctcagag gattggtcaa agtttggcct ttcatctcgt gcaaaaaatg
     4921 cgtaatatac gccgccttgc agtcacagta tggtcatttc ttaactcatg cgcatcggac
     4981 aatatcagct cagaaatcgc ctgatcgcag cgcccatggc tggcattaca gacagacctt
     5041 ttcggacgtt gtgctacgag atgggagccg gattgacagt atccgagatg atgtcttcta
     5101 acccacaggt ttgggaaagc gacaaatctc gtttacggat ggtgcacatt gatgaacccg
     5161 gtattcgcac cgtgcaaatt gctggtagcg atccgaaaga aatggcagat gcagcacgta
     5221 ttaacgtgga aagcggtgcc cagattattg atatcaatat gggttgcccg gctaaaaaag
     5281 tgaatcgcaa gctcgcaggt tcagccctct tgcagtaccc ggatgtcgtt aaatcgatcc
     5341 ttaccgaggt cgtcaatgca gtggacgttc ctgttaccct gaagattcgc accggctggg
     5401 caccggaaca ccgtaactgc gaagagattg cccaactggc tgaagactgt ggcattcagg
     5461 ctctgaccat tcatggccgt acacgcgcct gtttgttcaa tggagaagct gagtacgaca
     5521 gtattcgggc agttaagcag aaagtttcca ttccggttat cgcgaatggc gacattactg
     5581 acccgcttaa agccagagct gtgctcgact atacaggggc ggatgccctg atgataggcc
     5641 gcgcagctca gggaagaccc tggatctttc gggaaatcca gcattatctg gacactgggg
     5701 agttgctgcc cccgctgcct ttggcagagg ttaagcgctt gctttgcgcg cacgttcggg
     5761 aactgcatga cttttatggt ccggcaaaag ggtaccgaat tgcacgtaaa cacgtttcct
     5821 ggtatctcca ggaacacgct ccaaatgacc agtttcggcg cacattcaac gccattgagg
     5881 atgccagcga acagctggag gcgttggagg catacttcga aaattttgcg taaacagaaa
     5941 taaagagctg acagaactat gttcgaacaa cgcgtaaatt ctgacgtact gaccgtttct
     6001 accgttaact ctcaggatca ggtaacccaa aaacccctgc gtgactcggt taaacaggca
     6061 ctgaagaact attttgctca actgaatggt caggatgtga atgacctcta tgagctggta
     6121 ctggctgaag tagaacagcc cctgttggac atggtgatgc aatacacccg tggtaaccag
     6181 acccgtgctg cgctgatgat gggcatcaac cgtggtacgc tgcgtaaaaa attgaaaaaa
     6241 tacggcatga actaattcag gttagctaaa tgcttgatta aaaaggcgct actcggcatg
     6301 gggaagcgcc ttttttatag gtgtcacaaa gggagtgacc atgagaacag gatgtgaacc
     6361 gacccggttt ggtaatgaag ctaagaccat tattcacggt gatgcgcttg ccgaacttaa
     6421 aaagatcccc gccgaaagtg tcgatctgat ctttgccgac ccaccatata acatcggtaa
     6481 aaattttgat ggtctgatcg aagcctggaa agaagatctg tttatcgact ggctgtttga
     6541 agtgattgca gagtgccacc gcgttctgaa aaagcagggc agcatgtaca tcatgaacag
     6601 cacggaaaac atgcccttta tcgatctcca gtgccgcaag ctttttacca tcaaaagtcg
     6661 catcgtctgg tcatatgaca gttctggagt acaggcgaaa aaacactacg gctccatgta
     6721 cgaacccatc ctgatgatgg tgaaagacgc aaagaactac acattcaacg gtgatgctat
     6781 tctggtagaa gccaaaaccg gatcgcagcg cgcgttgatc gattatcgca aaaatcctcc
     6841 acagccatac aatcatcaaa aagtaccggg taacgtctgg gattttccgc gcgtgcgtta
     6901 tttgatggat gaatatgaga accacccgac gcaaaaaccg gaagccttac tgaaacgcat
     6961 tattctcgcc tcttccaacc caggcgatat cgttctcgac ccgtttgccg gtagctttac
     7021 taccggtgcc gtagccgtct ccagcgggcg aaaattcatt ggtattgaga tcaacagcga
     7081 gtacatcaaa atggggcttc gacggctgga tgtcgcgtcg cattactctg cggaagaact
     7141 ggcgaaagtg aaaaaaagaa agaccggcaa cctgtcaaaa cgaagccggt taagcgaagt
     7201 tgaccccgat ctcattgcaa agtaaaggaa gtgtaagcct ggttttcaga ttattcattt
     7261 cgtgtatatt tcctgccaga cttggttaaa catacacagg ctcaggtaat gattcgcaag
     7321 tattggtggc tcgtcgtttt cgctgtcttc gtttttctgt ttgatacttt actgatgcag
     7381 tggattgaac tgctggcaac agaaacagac aaatgccgca atatgaactc agttaatcca
     7441 ctaaaactgg ttaactgtga cgaactgaat tttcaggaca gaatgtgaat ttacatgaca
     7501 cttaattcat tcgtttgatg aattaatttc gttatgtttt catctggcat gaacattctt
     7561 aatacgttat cgaccagagc gggggcttgt ttataaagat cataacccgc catattcatt
     7621 aaccagtttt gaacaattcc gctgaaggca ccattaataa taatcatcac aacatctaaa
     7681 tcgaggttat ttgctacaca accttgttgc tgacacgcct gcaatacttc gcggagagtc
     7741 tgcggattga agcccatctt ttcgcgtatc actccctcgg ccagcatctc atcattaaat
     7801 tcacatttgt gatataagat tttcagcaac gcctgctggc ggggaatttt ggcaatatat
     7861 tgcaagccga caatcaattt ttcacgcaat tgttgaaacg ggtcatgctc taatccagcc
     7921 gtcaagtgtt cctggattaa ctcccgcaat gaaggctgtt gcaaccacat ctcattaaac
     7981 agttgagtct tgttttcgaa gtgccagtag atagcgccac gcgtaacgtt agcagcgtcg
     8041 gcaatgtcgt tgagtgtcgt cttgcttacg ccatgctgcg caaactgggc gatggcagtt
     8101 tcaatcagtt cttgccgggt cttcagagct tcggctttgg ttctttttgc catgattaat
     8161 tattcaggaa ataaatatat tcgacacaga gtgagaaaat agcgaaggtt aatctatcac
     8221 ctaatgtgta tttatacgag aggctaatat tgagttgcta taaatcgtca aataaataat
     8281 atatattatt tacctaaaat acattcgcca catgaatata tatttcaatt tacgaggttt
     8341 taattctgcc tctttcaacc cgcgtcaaaa taaaacagta gaatattaat ctttttttgt
     8401 gtttatgtgc cttgagacgc cagtattcat aactattcct tacatcgacg aatgataatt
     8461 tgtaggatag caaactgtat ttttctttct gcgagttaac gcgttgcctt tctgggtaaa
     8521 taacgcgctt ttggtttttt gaggaatagt aatgacgaaa catgccaggt ttttcctcct
     8581 gccctccttt attctgatct ccgcggcttt aatcgccggt tgtaacgata agggagaaga
     8641 gaaagctcac gtcggtgaac cgcaggttac cgttcatatt gtgaaaacgg ccccgttaga
     8701 agttaagact gaattaccag gccgcaccaa tgcttaccgt atagccgaag ttcgcccaca
     8761 ggttagcggg atcgtactga atcgtaattt cactgaaggc agcgatgtac aagcaggcca
     8821 gtccctgtac cagatcgatc ccgcgaccta tcaggcaaat tatgacagcg cgaaaggcga
     8881 actggcgaaa agtgaagccg ccgccgccat cgcgcatttg acggtaaaac gttacgttcc
     8941 gctcgtgggt acgaaataca tcagccagca ggagtacgac caggccattg ctgatgctcg
     9001 ccaggccgat gccgccgtga ttgccgcaaa agccacagtc gaaagcgctc gcatcaatct
     9061 cgcttatacc aaagtgactg cgccaattag cggacgtatc ggcaaatcga ccgtgaccga
     9121 aggcgctctt gtcactaatg ggcaaacgac tgaactggcg actgtccagc agctcgatcc
     9181 tatctacgtt gatgtgaccc aatccagcaa cgattttatg aggctgaagc aatccgtaga
     9241 gcaaggaaat ttgcataagg aaaacgccac cagcaacgta gagttggtca tggaaaacgg
     9301 tcaaacctat cccctgaaag gtacgctgca attctctgat gtgaccgttg atgaaagcac
     9361 cggctccata accctacgtg ctgtcttccc taacccgcaa catacgcttt tgccgggtat
     9421 gtttgtgcgt gcacggattg atgaaggcgt ccaacctgac gccattctta tcccgcaaca
     9481 aggcgttagc cgcacaccgc gtggtgatgc aaccgtgctg attgttaacg ataaaagtca
     9541 ggtggaagcg cgccctgtcg ttgccagtca ggcgattggc gataaatggt tgattagcga
     9601 aggtctgaaa tctggcgatc aagtcattgt cagcggcctg caaaaagcgc gtccgggaga
     9661 gcaggttaaa gccactaccg atacccccgc agatactgca tcgaagtaag gtaatctgac
     9721 atggcaaact tttttattcg acgaccgata tttgcatggg tgctggccat tattctgatg
     9781 atggcgggcg cactggcgat cctacaattg cccgtcgctc agtatccaac cattgcaccg
     9841 cctgcggttt ctgtttcggc aaactatcca ggtgctgatg cgcagaccgt gcaggatacg
     9901 gtgacgcagg ttatcgaaca gaatatgaat ggtatcgata acctgatgta tatgtcctcc
     9961 accagcgatt ccgccggtag cgtgacaatt acccttacct tccagtccgg gaccgatcct
    10021 gatatcgcgc aagtgcaggt gcagaacaaa ctccagctcg ccacgccgtt gctgccgcag
    10081 gaggttcagc agcaggggat cagtgttgaa aagtccagta gcagctattt gatggtggcg
    10141 ggctttgtct ctgataaccc agacaccaca caggacgata tctcggacta tgtggcctct
    10201 aacgttaaag atacgcttag ccgtctgaat ggcgtcggtg acgtacagct tttcggcgca
    10261 cagtatgcga tgcgtatctg gctggacgcc gatctgctaa acaaatataa actgacaccg
    10321 gttgatgtga ttaaccagtt gaaggtacag aacgatcaga tcgctgccgg acagttgggc
    10381 ggaacgccag cgttaccagg gcaacagttg aatgcctcga ttattgctca aacgcggtta
    10441 aaaaatccgg aagaattcgg caaagtgacc ctgcgcgtaa acagtgacgg ctcggtggta
    10501 cgcctgaaag atgtcgcacg ggttgaactt ggcggtgaaa actataacgt tatcgctcgt
    10561 atcaacggaa aaccggcggc gggcctgggg attaagctgg caaccggcgc gaatgctctc
    10621 gataccgcga aagccattaa ggcaaaactg gcggaattac agccattctt cccgcaggga
    10681 atgaaggttc tctaccctta tgacaccacg ccattcgtcc agctttctat tcacgaagtg
    10741 gtaaaaacgc tgttcgaagc cattatgctg gtgttcctgg tgatgtatct gttcttgcag
    10801 aatatgcgag caacgctgat ccccaccatt gcggtacccg tggtgttgtt agggacgttt
    10861 gccatcctcg ccgcttttgg ttactccatc aacacactaa cgatgttcgg gatggtgctt
    10921 gccatcgggc tgctcgtcga tgatgcgata gtggtggtgg agaacgtcga gcgcgtgatg
    10981 atggaggata agctcccgcc aaaagaagcg acggaaaaat caatgtcgca aatccagggc
    11041 gcactggtgg gtatcgcgat ggtgctgtca gcggtattta ttccgatggc attcttcggc
    11101 ggttctactg gggcaattta tcgccagttc tctatcacca tcgtttcggc aatggcgctt
    11161 tctgttctgg tggcattgat tcttacccct gcgttatgtg caacgctgct taaacccgtc
    11221 tctgctgagc atcacgaaaa taagggcggt ttcttcggct ggtttaatac taccttcgat
    11281 catagcgtta accactacac caacagcgtc ggcaaaatcc tcggttccac aggacgatat
    11341 ttactgatct atgcgctgat tgttgcagga atggtggtgt tgtttttacg tcttccgtct
    11401 tccttcttac ctgaagagga tcagggtgtc tttctgacca tgattcagtt acccgctggc
    11461 gcgacgcaag agcggacgca aaaagtgttg gatcaagtga cggattacta tctgaagaac
    11521 gagaaagcga acgttgaaag tgtctttacg gttaacggct ttagcttcag cggccaggca
    11581 caaaacgccg gtatggcctt cgtcagtctg aaaccgtggg aagagcgtaa tggtgatgaa
    11641 aacagtgcgg aagcggtaat ccatcgtgcc aaaatggaat ttggcaagat ccgcgacggt
    11701 tttgtcattc cattcaatat gccagccatt gttgaactgg gcacggcaac gggtttcgac
    11761 tttgagttaa ttgatcaggc tgggctgggt cacgatgccc taacccaggc ccgtaaccag
    11821 ttgcttggta tggcggcgca acatcctgcc agcttagtca gcgtgcgtcc taatggcctg
    11881 gaagacaccg cgcagtttaa actggaagtt gaccaggaaa aggcgcaggc attaggtgtt
    11941 tcactttctg acatcaatca gaccatttca acggcgttgg gtgggactta cgttaacgac
    12001 ttcatcgacc gtggtcgcgt gaaaaaggtg tatgttcagg cggatgccaa attccgtatg
    12061 ctgccagaag atgtcgataa actttatgtc cgcagcgcca acggcgaaat ggtgccattc
    12121 tcggccttta ccacttcaca ttgggtgtat ggctctccgc gactggaaca ctacaacggt
    12181 ctgccgtcaa tggagattca gggggaagcc gcgccaggaa ccagttccgg cgatgccatg
    12241 gcgttgatgg aaaaccttgc gtcaaaatta cctgctggca ttggttatga ctggacgggt
    12301 atgtcgtatc aggaacgctt atcgggaaac caggctcccg ctctggtagc aatttccttt
    12361 gtggttgttt tcctgtgcct tgctgcactc tatgaaagct ggtcaattcc tgtctcggtt
    12421 atgttggtcg tgccgttagg gattgtcggc gtgctgctgg cggcgacact ctttaatcaa
    12481 aaaaatgacg tctactttat ggtgggcttg ctaacgacaa ttggcttgtc ggccaaaaac
    12541 gctattttga ttgttgagtt cgctaaagat ctcatggaga aagagggtaa aggtgttgtt
    12601 gaagcgacac taatggcagt acgtatgcgt ctgcgtccta tcctgatgac ctctctcgcc
    12661 tttattctcg gcgtattacc gctagctatc agtaacggtg ccggcagtgg cgcgcagaac
    12721 gcagtgggta tcggggtaat gggcggaatg gtctctgcaa cgttgctggc aatcttcttc
    12781 gtaccggtgt tctttgtggt gatccgccgt tgctttaaag gataaatcag aaacataaag
    12841 gcactttcgg gtgcctttat tattttcagt gaaacccata aaaattaata agatattctt
    12901 ccactcactc
//
LOCUS       DAZGTR010000120        12882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGTR010000120 DAZGTR010000000
VERSION     DAZGTR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.122"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(56..124)
                     /locus_tag="ACHLCY_003846"
                     /pseudo
     CDS             complement(56..124)
                     /locus_tag="ACHLCY_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(648..1307)
                     /locus_tag="ACHLCY_003847"
                     /pseudo
     CDS             complement(648..1307)
                     /locus_tag="ACHLCY_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
     gene            complement(1324..1605)
                     /gene="gamL"
                     /locus_tag="ACHLCY_003848"
     CDS             complement(1324..1605)
                     /gene="gamL"
                     /locus_tag="ACHLCY_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFP0371018.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRAQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(1626..1907)
                     /locus_tag="ACHLCY_003849"
     CDS             complement(1626..1907)
                     /locus_tag="ACHLCY_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0371019.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVVLSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(2202..2876)
                     /locus_tag="ACHLCY_003850"
     CDS             complement(2202..2876)
                     /locus_tag="ACHLCY_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP0371020.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSDNHPYATMNYVDRKVISLDA
                     SVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRF
                     GFNVNL"
     gene            complement(3132..3905)
                     /locus_tag="ACHLCY_003851"
     CDS             complement(3132..3905)
                     /locus_tag="ACHLCY_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0371021.1"
                     /translation="MGPTSVGLVPLIASCSNPVQSTASELATSSGGYQSPIKEAAIMA
                     TIPVLSQPEITISNGRAVTTSFAVAEYFCKRHDNVLRAIANIECSEKFNALNFEDVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(4534..5487)
                     /locus_tag="ACHLCY_003852"
     CDS             complement(4534..5487)
                     /locus_tag="ACHLCY_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFP0371022.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYSTE"
     gene            complement(5484..6953)
                     /locus_tag="ACHLCY_003853"
     CDS             complement(5484..6953)
                     /locus_tag="ACHLCY_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFP0371023.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGLGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGRQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTLEKSTIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(7048..7761)
                     /locus_tag="ACHLCY_003854"
     CDS             complement(7048..7761)
                     /locus_tag="ACHLCY_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP0371024.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            7857..8060
                     /locus_tag="ACHLCY_003855"
     CDS             7857..8060
                     /locus_tag="ACHLCY_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0371025.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGTVEAEEVRPFPAFVRGDD"
     gene            8115..8384
                     /locus_tag="ACHLCY_003856"
     CDS             8115..8384
                     /locus_tag="ACHLCY_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371026.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            8592..8969
                     /locus_tag="ACHLCY_003857"
     CDS             8592..8969
                     /locus_tag="ACHLCY_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371027.1"
                     /translation="MSAATKSALIRTLSTVPLRTEERYSFLADVVTILESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            8963..10483
                     /locus_tag="ACHLCY_003858"
     CDS             8963..10483
                     /locus_tag="ACHLCY_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0371028.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDKDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYTREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAN"
     gene            10473..11444
                     /locus_tag="ACHLCY_003859"
     CDS             10473..11444
                     /locus_tag="ACHLCY_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFP0371029.1"
                     /translation="MRTRDAAVGHWSRIFEYYGMPPVTGVKHYNGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMNLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRELVIDKFAGLPCLRGTTGEAYLQGRGILQLPTESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSCNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            11444..11893
                     /locus_tag="ACHLCY_003860"
     CDS             11444..11893
                     /locus_tag="ACHLCY_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFP0371030.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNNPELNNRLLSFF"
     gene            11901..12464
                     /locus_tag="ACHLCY_003861"
     CDS             11901..12464
                     /locus_tag="ACHLCY_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0371031.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYQKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERDRHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            12461..12655
                     /locus_tag="ACHLCY_003862"
     CDS             12461..12655
                     /locus_tag="ACHLCY_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP0371032.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            12648..>12882
                     /locus_tag="ACHLCY_003863"
     CDS             12648..>12882
                     /locus_tag="ACHLCY_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0371033.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLG"
BASE COUNT         3187 a         3013 c         3375 g         3307 t
ORIGIN      
        1 tcacctcaaa taagtggttt gctgcctaat ttcattttct ggcgaccaac acaagtcatc
       61 ttgccgtcag ttgttttgat ttccggtagc ctgccgcgta aagagctacg tttggaagac
      121 atacaccagt ttctggttgc ttatgtccaa actcattcgc gtacacaatg gccgctcgct
      181 ccagattgcg tctgtattct ttctgttgcc agatcacgtc ctgtgccatg aacttaattg
      241 gcttagcgtc ttctatgcgc tcaggcgttt cgtgagtacc tttagcctga atctgcgctc
      301 tgcttagagt agggcggtgt aatacttctg aacttattgc ttcttcgcgg gccagcacgc
      361 cgttagctaa tgcctttgcc tttaaacgct cacgacgacg agaacgtgaa ttgcctttga
      421 actgagttct gcgtgtcata tagacctcct gatgaacttt ggtggtgtgg taggtgggag
      481 acccatttcg acctgtttcg gcctacttca attcggcaat agtcccgcag gcctcgccgc
      541 tttacgtgcg acatattccc gtccatgaac ccttcaccac accccaaagt tcactttggt
      601 tattgcgctt tgtcagcgcc gtagattcat attcgaatcg ttgtatattc accgccctga
      661 gtgttcaggt agtcgatagc tgtatccagg cgatcgcgac ggggccagta tttgctggcg
      721 cgcttaacaa tggcttttct ggccatttca tcggggaaac tatcccacgg gctacttcct
      781 ccgttatttc cggctttgct gcacgccctg atgacctcga tttccctgtt gctcatctct
      841 tcggtcagat agtcgccttc ggatgttttt acgacacaat aaccgccaat gcgtgcgccc
      901 ctgtctacga aggggttgta tttgtgcgtg ggggcgcagt caataccgtt ggactcgtaa
      961 atgtccttct cgtaaacaag tttgcattgc ccccactgaa tggctcctgt gacctgtgcc
     1021 agatgcagaa gccccatata actgatatcc aggcatacag ccttttttcg tgggaccaga
     1081 tacgccagct tgctggccgg gttcagggtt atgccgatgg cggcaacatt gatgatagcg
     1141 ttctgtgcgc tgggcagatt tgcccttgct gtgtcagcca gaaaggcatt tttctggaat
     1201 tgctgaattg caaactgact ttccttcgcc catgtcagcg tcggttcagt taatgcctcg
     1261 cagaaaaagc gctcctgctg cttaacaaat tcaacgatat cgaacatttt ttggtcctga
     1321 aaatcagaaa ggacaggggg agaattttct ctcccattct tcttccgccc gagcataggc
     1381 gatcgctgag atataatcgt tgtacgcctc ttcagctttt tcgccagtga gtgctagttg
     1441 ggcttctttg ggtaaaaaaa ggctgctcat aagcaatggt ttatcgggga acatgctgat
     1501 aagctcctgc gcccgatcat caatccattt atccttttca tcctgaattt gctgattaat
     1561 ccagcgacgc tcctctatgc ggtcgcaggt gaggtatgcg ttcatggcgg aactcctgat
     1621 tccggttaat gcattaaatt aatttgtcgg gaaagctgac atacagggca gttacattct
     1681 tcctcctgct ccttagcgaa gaaatatgca gcggcctgta atgcgatatc ttctggatgt
     1741 tccgcgataa acataacatt gccttccgta tcaataacag aaatagcctc atcagacagg
     1801 acgacaaaat aggcgatgat tttatcatcc ataaaaactt ctcccattat cgttcctgct
     1861 ggagttacga cgctttttac attgatattt attttttgat tgagcatgat atttcccttc
     1921 aggctggtga gattaacggt tggcctttat tgttcaggta aacttctatt gcatctgaga
     1981 taatgcgaat tttttcaatc agtgaatgtg cgtaaagtgc attattaacg ttcgctgacg
     2041 ccatgtaata acgcccgttg taaagaattg ctgtgccggg tttaacgtcc tcgcgagaaa
     2101 ctaatgcggt tccgtagtga ggtttgagca tgacaaatcc tccggttaat taatccagat
     2161 atttaattta atccccgata tgtggtcggg gatggggtta attaaagatt tacgttgaaa
     2221 ccaaagcggg aagacttttc tgatgtacgg gaaatatcca gcaatttacg gcgcatttct
     2281 tccgtcagat gcttgaaggc gttaaattcg gcgacagcgg cgtcaacgtt atagcctttg
     2341 ctgtgtagct cattgaggat gcgcatagtt gggcttggca tatcgaagag cacggaagca
     2401 tcaaggctga taaccttgcg atcaacatag ttcatcgtgg cgtagggatg gttatctgag
     2461 aaccacgaga gagggtagtt gatagatagc gttgattctg gaagctgaag ttgttctggg
     2521 attttttgac tgaaatagca gtcttccagt ttttcgaaca cttcccacgc ccgatcggtt
     2581 tcgagcattt ttgcgtgacg ggctgcgccg cgttctgtcc agaggatgag ggagcgaacg
     2641 ttacgggcga ttttcacaga aggtcttaaa gacatgctgt gcttaaattc ccgcaactcc
     2701 tcattctcaa gtaaaaagta gtgcttaccg ataacaaatc gtccagaatt ccgattgaaa
     2761 ttttttcgga tgtaatctgg ttctgtgcca tataagctgg caagaagctc tgtagtcacc
     2821 acaggtatat tactaaatga aattatggag agggactcca cggagatggt tttcatgctt
     2881 tgcgtcctac ttgatatttt gatttacccc ttttgagagg gtgaccgggc gctcaaaacc
     2941 gcaagtagtc ggcgggcata ttcccattgc tggtattgta ttagccgcac gcccggtcat
     3001 aaaccaagaa gactggacac aaaaaaaccg catgtctgtc gggcgcggta accgctactt
     3061 gctaaggtgt tttgagcacc atgaggcaga atatatcccc gttaatgcag cattgtcaaa
     3121 cgatctgttc tttaaagact taacgagaag ccttttcggg aatttcgatc tgacagtctt
     3181 gaaatatcct gtagcgtgcg gcgcatttct tccgtcagat gcttgaaggc gttaaattcg
     3241 gcgacagcgg cgtcaacgtt atagcctttg ctgtgtagct cattgaggat gcgcatagtt
     3301 gggcttggca tatcgaagag cacggaagca tcaaggctga taaccttgcg atcaacatag
     3361 ttcatcgtgg cgtaggggtg atgctctgaa aaccacgaca aagggaaatt gatattcatc
     3421 gcaggagaag acagggccag ttgtttctgt tcccatagtt gtttttccat gcgatcgaat
     3481 tcagcaatgt aggcttcctt gaatgcagct gcttttttgc cggtgaagcc catcaccaga
     3541 aaaacgaagc cgtttttggt gatttggtac attgggcgtt gttcgccttt ggcgtcggtg
     3601 taggtaacgt cctcaaaatt gagggcgtta aatttctctg agcattcaat atttgcaatg
     3661 gcgcgcaata cgttatcgtg tcgcttgcaa aaatattcgg caacggcaaa ggacgtagta
     3721 acagcgcgac cattggaaat ggtgatttca ggttgagaaa ggactgggat agtagccata
     3781 atggcagcct ccttgattgg tgattgataa ccaccgctgg aggtagcaag ctcgctggcg
     3841 gtggactgta cagggttgct acaactggca atcaagggaa ccagcccgac cgaagtcggc
     3901 cccatacagc ccaccattga taagatgtgc gtgtatgtcg atacaaaaaa agacgctggc
     3961 gcgtctgtat cgcctcgatt gtcagcgggg tagcaatccc gacacccgtt ttatgaggtg
     4021 ttcgccaaat atagccccga catcacacgc agtcaatacc gttctttctc agaaacgctt
     4081 tggcggtgcc gcctgcaccc caaaacattc cctgtattgg tcagcgccaa cttcctgcca
     4141 gtgttgcccg ttctcacgcc gttctcgctc tcgcgcgggg atactctctc atcgaccgga
     4201 tcgcacccga tgatacagca cgtttacgtg taggggtcta aacaggtcat tgacgctgta
     4261 aagctccaga ttgttaaaga gcattttgcg gcgggttaag tcgcgccgta cgactgattt
     4321 atgtagccct gtgtaagggc gcgatgtttc tggcttgaaa taaatataac ttgcggtgat
     4381 tttcatgtaa ataccgttgg tgctttttaa gggcgagaaa ttattactta actgattttt
     4441 aaagtgattt attttttaag ggggcagata acaggagggg atatgcaaag aaaaacccgg
     4501 cgcatgggcc gggctattta tctggtcctg ttctcattca gtagagtagg ggccaagaaa
     4561 tcgcactccg ttgaagtgaa taagatgaga aggtgcatca gccacccata cctctgtttc
     4621 ccatgcgatt tcaccaagat atcgccccat gatggagcga tttggaaaag cggtcacata
     4681 gacaagtccg gctgttgatc cggcaaacag cctggcaagc tcagcatgcc gcttcccatc
     4741 aaccggtcca tgactggtga cagactcaac cagtagcagc cagtttttcg cagtaaaatg
     4801 tagcaccaca tctggcattt taccgtgtga atccacatca acacctagcc cggccagcag
     4861 tggagcgtcg aagtagcccc acttttcgcc agtgtcgcca gcatagacca gcacgctacc
     4921 cggagcaaaa cgtggggcga agtcctctat tatggcacgg ataagttcgc tgtgctcgcc
     4981 gggactgagg gttatttgct ggcccgcagc aatttcaaca gggatacgat tctgttcgcg
     5041 ctccttagca tagcgggtaa ccagcgtttc acgctcggct aaataggttg caaggctatc
     5101 gtgccatgcc gggctgccga aagtgcgtag catggacaga gcagcaggtt cgatctgata
     5161 aacagccttc gggctgttca ctgggcgatc gggcttgtcc ggattgtaga gggctactcc
     5221 agcagcgcag aactgatgca tagactggcg gcggaatgtc tcacgagtat tgggtgcgta
     5281 gaccttgccg tagtgctccc gaacccaatt catgattggt gtaatgccca caagcggatt
     5341 ttccgcgtcg gcccatgctt ttcccggtgt gaggttcagg agtgccagca aacacaaagc
     5401 agaacgctca ttctgctgcg ctctgggcag gcctaaggat gctataattt gctgagcggc
     5461 ctcaatataa tcattttggt tgttcatgca gtcagagctc ctagcttagc gtcaatatgt
     5521 tcttgcgtga gagtttcttg ttgcatagcc cacttgccaa gctctatcag agtgtcacgg
     5581 cttgggtact taatcatctt gaggtcggtt gcattgacct gtgtatgccc gttgaagcga
     5641 cggaaatact tatccactgc ggtagtgttc aggaacacgg ccaagccacg ggccagcgcc
     5701 tcgggtaacc catgtttgtt ctcatgaaac acgttcatgt gattttcaaa tcccaacact
     5761 gaatactcac tgaaggtagc aggatcaacg acgcttgcta ccactcggcg cttttcctcc
     5821 ttcgacgaga aacgacgcac cacacaataa aaaccgttcg ggtaaagcca cttttctgtc
     5881 tcgtcgttgc gcatgattgc gttgggtttc ttcaaacctt ccacaggcca gacagtgcta
     5941 ctcatactca aatggctcgg gtagatcagg ggaacagttc cttcttctgg catgctgcgc
     6001 aaatgagctt tcagtcggaa atcaactatc ggtccggtcg ataccttcac gccaatatca
     6061 gccagcgaac attgtacggc agatgataac tcaatggtgc ttttctcgag cgtggtcggc
     6121 acatgaataa accgctccga atcgtccggg tacacaatct ggtcaaatgg gtgttcgtag
     6181 gtgactaggt caaaaaagct atcatcggtc gaagttgtga ctgtaacagc ctcctgacgg
     6241 cctccacgct ccaggcggat gataatattt tcctgcaaca catcgtcatc cctgaaagct
     6301 ttgctacgtg attcgaacaa atgaatatgg cggattgctg cccgctcaag tatgaaatta
     6361 cggaacggac ggtaatacgg tccgttgcag aaactacgtg ggattatggc cactatctgc
     6421 ccgccaggtg caacctcacc tacagccagt gcaacaaagg cggaatacag atttaccgtc
     6481 tcgataccga cagtgcgaag agcctgccgg tgggctgact ggctattgat cttcttgtaa
     6541 ggcggattaa gtattgcatg ggtataacct cgatcctgta gaccttcggc ggttgccagc
     6601 tcaatataat ccccttcgat gatgcggggc gtcactctcc tgtacccggt taagtgcttc
     6661 gcaaggtgac cacacagctt tttgtcgatt tcgtaggctg ttgcctcgac agactcaaag
     6721 ccaaactcgc caaccagcca acggtcgagg aaggcgcaag acagtgcgcc taaaccagcg
     6781 cctgcgtcca gtagacggca agttttcatt gtgctcggtg gaaacaagga ggccataaaa
     6841 cgggccacgc cagaaggcgt catgaactgg ccgaactctg ctttgtgttt acgcgcggta
     6901 cggggagaca gctcgcgacg cacgctgtct gcgacgtcta gcttttgaag cataactaat
     6961 cctctttacg tggcatgatg atgccaacgt gcttttgaca tcctccacat tcttcaggcg
     7021 tgaaggatgt caaatcactc tgtgagctca atcaaaagtc tcatcattcc actgcgattt
     7081 gatgaccttt ccgacaaact ggcagttttc gttacactca atcatgtcga atcgtgggtt
     7141 gagcggctcc aggtacgctt taccgctctc acgaatgaat cgtttgaagg taaattcatc
     7201 gccgttcatt cgggccacgc aaaaatcgcc atcctcgatt tcccgttcag ggtcaacgag
     7261 tatcagcata ccttcaggga agctgggttt accaccctgc ggtgcggtca ttgaatgccc
     7321 cttgacctcc agccagaacg acctgtcact ggcttttgtg gtggtggaga tccatttaat
     7381 cgcatctttt gcggtgtacg aaatatcatc cattgagaat gcgccagctt gtacgcagga
     7441 gagtaacgga tattcgtaag cggggacacg agttaatggc tcttcaattg cgagcgccat
     7501 ttctgcaata gattttgcca ggctgggact gaaatctccg acctttacat tcagtatctt
     7561 agcaagctgt gctgcgtggc tggcatttat ggcgttgatg ccagccatta gctgattaac
     7621 agcgctttgg gtaacaccta attcataggc taatgattcc tgcgacaggc ctaactcttt
     7681 ctttttagcg ttaaaaatac ttttcagacg cttggcgtct tccagttgct caggggttaa
     7741 cggtttcttt ttcatgttca taccatatca cctttggtta tatgtaatga aatatctgcg
     7801 gtgttgacat tttaataact gtgcgtaatg atatgggcat tttgaaggag tcagttatgg
     7861 cgatgaaaaa agagaccctg gctgattacg tatacaggtt cggccaaaag aaagccgcta
     7921 aggactttgg cgtggcgcag agtgcaatca gtaaagcgct tctcgtcgga cgagaaatat
     7981 acgtcaaaac ctttgatgac ggcacagttg aggcggaaga ggttcgtccg ttccctgcgt
     8041 tcgtaagagg cgatgattaa tcctggacac acagcccatc ggaggtgtga gtgaacaggc
     8101 aaatcaaaac ggttatgccc gagcagtact gtgatgctga tcgggactgg atacaggagc
     8161 aattgttaca gctagacccg accacccggg taaaaattgc agcagagtac gcgagagtgt
     8221 accaggaaga gtgggacaag gagcctgtat cgttcaggaa gggcaacagg gcaagacgga
     8281 gcgccaacac ccggttacgg gtgtatgtcc agaagtacgc gagagccagc cgtggctgga
     8341 tgctttcgcc agtagcggta cggaagtttc ccccgacacc atgatggcgg ttagttgcgc
     8401 tagagataat ttcaagtgca actaatttcc tgaaaggaag cgaggagggg gtaagggggt
     8461 ggagtcgttg gtaaggcctg tatcccttgg cctaggccag aaggcaggta cataggttag
     8521 gtagatcact gtataagcgc gcgatagttt ctggtgaaat gattatcgag ctaactattg
     8581 agtgataaac tatgtcggca gcaaccaaat cggcactgat ccgtacactg agcactgtac
     8641 cactgagaac agaggagcgt tacagctttc tggctgatgt tgtaacgatc cttgagtcac
     8701 aggggatgca tgtggcaagc aatgtcacag taagaatcga tggcagaaat ttccgtgttg
     8761 atattctcgc aacagcaaaa actggtggga gtgtggctat cgagatcgat cgtagctctc
     8821 cgcgaccccg ctcagtcatg aaattgcgtg aattggcacg tcgtggcact gaaggctttg
     8881 tgttgcttcg aatgccaaag aaactgacca gttatagcga tgctgggatc gatatcatcc
     8941 cggcgaatgg caagggggcg tcatgctgaa aatcacccca aactttgctc aggagcgcgg
     9001 tttaaaccag ctgcggcacc agtggaaaca gcatcgcacg tacctgatgt atgcacccac
     9061 tggctctggc aaaactggtc ttgcagcatt cgtcactgcc ggaatggttg aacgtggtat
     9121 gcgggtgatg tttgtctgtc cgtacaccat cctgctgaac cagacagcgg aacgtttcac
     9181 cgaatacggt ttgccgtggg aagaaatcag ttttgtatgg cgtgatcacc ctaactatga
     9241 cccgtcacga ctgattcaga ttgccagtgc cgacacgctg atccgtcgtg agtttcctga
     9301 caacatcgat ctgctgatca tcgatgaagc acacatgaag cgtcgtgcgt tacttgaggt
     9361 tatccgggac aaagatatca gggttcttgg gctttccggc accccgttcg cagcctggat
     9421 ggggaagtac tacgaatgcc tgatcaagcc tacaacgatt cgtgaactta ttcagcgtgg
     9481 tgatctgagc gattatgagt tttttgcccc ctcaatgcct gatctggctg gcgtaaaaac
     9541 cagtaacacc gtatttggac gggattacaa cgaggaacag ctcgcatcca tcatggggag
     9601 ttcggatctg gttggcgata tcgtcagcaa ctggcttgag aatggcgagg atttaccgac
     9661 gatctgcttc tgcgtgaatg tggctcatgc gaactttgtt acccgtgagt ttttacagtc
     9721 tggcattggc gcagaggtga tgacggcaga taccccccat gatgaacgac aggacatcat
     9781 tcgccgtttt gaagagggtg cgaccaaaat aattgtcaac gttggcgtac tggttgcggg
     9841 atttgacagc gatgttcgct gcctgatcta cgcccgtccg accaaatcag aaatccgctg
     9901 gttgcagtgc atcgggagag cgttacgtac tgcgtctggt aaaaaacgcg cattgatttt
     9961 cgaccattcc ggcacggttc atcggctggg cttccccgag gatatcgagt atgacgaact
    10021 tccgggaaaa aatgacggaa tgaaagcgtc tgctggcggg agtgaggtta aggctgagaa
    10081 acttcccagg gaatgcccca aatgccactt catgaagcct gctggcgtgc atatgtgtcc
    10141 caaatgtgga tttcgtcctc ttggtggtga tgacgtggcg acagatcgcg atcgtaagct
    10201 ttcacgcgtc aacaaaggga aacgcgaata cacccgtgag gaaaaacagc gttggtggag
    10261 cgagatcaag gggtatcaga attaccgcaa cgcgacaggt aagccactga gtgacggatg
    10321 gtgtgctcat acctacaagg agaagttcgg ggtttggcct aaaggcttca gtaatgcgcc
    10381 gctgcaaacc tcagttgaag tgtacaactt catcaagtca aagaccattg cctatgccaa
    10441 agggcgcaag aaagccatga caggaggcca gcatgcgaac tagagatgca gccgtggggc
    10501 actggagccg gatttttgaa tattacggca tgcctcctgt tactggtgtg aaacattaca
    10561 acggaccctg cccgatatgt ggtgccagag gtaaatttcg ctgtgatgat aaggatggtt
    10621 ccggttcatg gatttgcgtc tgtggtcacg gggacgggat gaatctgttg caacttgcca
    10681 cgggtaagcc ctgggtgacg ttgtgtgatg aaatcgatcg gctgatcgga aatacctgga
    10741 agagggagaa agtcagccag cctgtaacag agataagcag aaagcgggag ctggtcatag
    10801 ataagtttgc cggactcccg tgtctgcggg gtacaacagg tgaggcgtac ctgcagggga
    10861 ggggaatact ccagttaccg accgaatccg tgcgtttttg tgaccgtcag atcgccagcg
    10921 ggcgcgaata tcaggcaatt tacgccattg caacagatga caaaggttct ctttgctatc
    10981 tgcatcgtac gttgctggat ggtgatcgca aggcgaatgt agaggcggct aaaaaaatga
    11041 ccgcgctaca ggagttgcct ggtttgcagc atgccaaatc ggtggcaata cgcctgtatc
    11101 cggtgtcgtc cactctgggg atagccgagg gtatcgaaac tgcgctttca tgccgtcaaa
    11161 tcttccgctg caatgtgtgg tcaacaatga actccggttt tatggagaag ttcattgcgc
    11221 cacctggcgt taatcacctg attatctttg ctgacaatga tgcgcacggc gcaggtctgg
    11281 cggctgcctt taaatgtggg cataagaatc tcatgagttg caatgacgtt gagaaagtca
    11341 gcattcgctg gcctgacttg ccggatttta acgacatgct cattcagggg tgtgaagccc
    11401 gtgaacatgt gttgacgcgc aaattcaaag cggaggctgc ctgatggaaa tagagatgat
    11461 caaggcggct aatggcgtat ttgtaccggc gtatgagcgc gatttacccc gactggcaaa
    11521 atttaaaaac ggtgagctgt atacactgga agcaaaactt acccgtaacc catcttttca
    11581 ccggaagatg ttcgcttttc ttaatttctg ctttcagtac tggtgcgctg aacatgctgg
    11641 atacgaattc tctgatgaag cgacgcagtt tgatgagttt cgtaaaaatc tgacaattct
    11701 tgccgggttc tatgatgtgg tcacaaccat aagaggcgag gtgagatatc gggcaaaaag
    11761 cctgagttac gcgaatatgg atcaggatga gtttgaacgt tgctacaacg caatgattaa
    11821 tgccgcgtta aaacatgtgt tcgggcgctc aaacaatcct gaactgaata accgcctgct
    11881 gtcgtttttc tgaggtgatg atgaagcaac gtaaaccaaa aaaatgcaaa gtgtgcggct
    11941 cctcgtttgt gccgttccgc tcatatcaga aagtttgctg tggtcagtgc gcactggaac
    12001 tggtcagaaa agaaaaggcg atagcttcag caaaagagca ggcagacaag ctgaaagcgc
    12061 gcaggaggga cttacagccc cgcagttact ggattaagca ggcacaacag gctgtgaatg
    12121 cttatatcag ggagcgggac cgtcatttgc cgtgtgtttc atgcgggacg ttcgattcag
    12181 cccagtggga tgcaggccat taccgtacaa cagctgcggc acctcagctc agatttgatg
    12241 aacgcaatat ccataagcaa tgcgtggtgt gtaaccagta caaaagcgga aatctcgttc
    12301 cgtatcgtgt cgaactgatt aaccgcatcg ggcaggaagc agtagacgaa atcgaatcaa
    12361 accatagtcg ccaccgctgg actgtcgaag agtgcaaggc gatcaaggca gagtatcaac
    12421 agaaacttaa agacctgcga aacagcagaa gtgaggccgc atgacgttca ccgtaaaaac
    12481 cattcctgac atgcttgttg aggcatatgg aaatcagacc gaagtggccc gaatactgaa
    12541 ctgcaatcgt gccacagtca gaaaatacat tggcgataaa gaagggaaaa gacacgctat
    12601 cgtcaacggt gttcttatgg ttcaccgcgg atggggtaaa gatactgatg cgtgatatcc
    12661 ggcaggttct tgagcgctgg ggggcatggg cggcaaataa ccatgaggat gttacatggt
    12721 cgcccattgc tgccggattt aagaggctga tccccgaaaa agtaaaatca cgtccacagt
    12781 gttgtgacga tgacgcgatg attatatgcg ggtgtatggc tcgccttaac aggaacaaca
    12841 gcgatctgca tgacttgctg gttgattatt acgtgttggg gg
//
LOCUS       DAZGTR010000121        12863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGTR010000121 DAZGTR010000000
VERSION     DAZGTR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.123"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(18..206)
                     /gene="bcsR"
                     /locus_tag="ACHLCY_003864"
     CDS             complement(18..206)
                     /gene="bcsR"
                     /locus_tag="ACHLCY_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP0371034.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            479..2050
                     /gene="bcsE"
                     /locus_tag="ACHLCY_003865"
     CDS             479..2050
                     /gene="bcsE"
                     /locus_tag="ACHLCY_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP0371035.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELMQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            2047..2238
                     /gene="bcsF"
                     /locus_tag="ACHLCY_003866"
     CDS             2047..2238
                     /gene="bcsF"
                     /locus_tag="ACHLCY_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP0371036.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            2235..3914
                     /gene="bcsG"
                     /locus_tag="ACHLCY_003867"
     CDS             2235..3914
                     /gene="bcsG"
                     /locus_tag="ACHLCY_003867"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP0371037.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVSDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELIDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(4001..4108)
                     /locus_tag="ACHLCY_003868"
     CDS             complement(4001..4108)
                     /locus_tag="ACHLCY_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0371038.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(4484..4591)
                     /locus_tag="ACHLCY_003869"
     CDS             complement(4484..4591)
                     /locus_tag="ACHLCY_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0371039.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            5067..6338
                     /locus_tag="ACHLCY_003870"
     CDS             5067..6338
                     /locus_tag="ACHLCY_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP0371040.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(6368..7372)
                     /gene="dppF"
                     /locus_tag="ACHLCY_003871"
     CDS             complement(6368..7372)
                     /gene="dppF"
                     /locus_tag="ACHLCY_003871"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP0371041.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(7369..8352)
                     /gene="dppD"
                     /locus_tag="ACHLCY_003872"
     CDS             complement(7369..8352)
                     /gene="dppD"
                     /locus_tag="ACHLCY_003872"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP0371042.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDIYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(8363..9265)
                     /gene="dppC"
                     /locus_tag="ACHLCY_003873"
     CDS             complement(8363..9265)
                     /gene="dppC"
                     /locus_tag="ACHLCY_003873"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP0371043.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(9275..10294)
                     /gene="dppB"
                     /locus_tag="ACHLCY_003874"
     CDS             complement(9275..10294)
                     /gene="dppB"
                     /locus_tag="ACHLCY_003874"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP0371044.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(10445..12052)
                     /gene="dppA"
                     /locus_tag="ACHLCY_003875"
     CDS             complement(10445..12052)
                     /gene="dppA"
                     /locus_tag="ACHLCY_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP0371045.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDALIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(12798..>12863)
                     /locus_tag="ACHLCY_003876"
     ncRNA           complement(12798..>12863)
                     /ncRNA_class="other"
                     /locus_tag="ACHLCY_003876"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2994 a         3429 c         3235 g         3205 t
ORIGIN      
        1 ggccattcac caatccccta cttttgttgc gcaaactctg ccagcaacgg ccagcgtttt
       61 aatgccgcgg ccaactgttc gcgttgggaa atatcggcat aatcaatatc aggcagtgaa
      121 aatgcctgct ttaacgccac aatatcattc tggaagatat agcctatcgc gggatcaggc
      181 agagtatctg gttcattgtt attcattttg cttgatccct atgaaataac cgtaccgttc
      241 atgagacaat tctaaccgtg agcatttcgc cggtttattg tttacggcga gacgggaatt
      301 tgctcacttt ttgactttcc gttacgcaac agaaaaagtt aaaaacgaaa agactaaaat
      361 cacacgtctt gtgaccttct aaattgattc acatgctaaa tctgataagt tttaatttca
      421 atggtaggtt tatttcttag ctttcgctag taaactgata atcagacaaa atggtgacat
      481 gagggacatt gtggaccctg tattctctat cggtatctca tcattatggg atgagctgcg
      541 acatatgcca gcaggcggcg tctggtggtt taacgtcgat cgccatgaag atgctatcag
      601 tctggcgaat caaacaattg catcccaggc tgaaaccgca cacgtcgcgg tcattagcat
      661 ggacagcgat ccggcgaaaa tctttcaatt agatgattct caagggccgg aaaaaataaa
      721 attattttca atgctaaatc atgaaaaagg tctatactat ttgacccgtg atttgcagtg
      781 ttctattgat ccccataatt acctttttat tcttgtttgc gcaaataacg catggcaaaa
      841 cattcctgcc gagcggcttc gctcatggtt ggataaaatg aataaatgga gcaggttaaa
      901 ccattgttcg cttttggtaa ttaatcccgg aaataataac gataaacaat tttcattgtt
      961 gcttgaggaa taccgttcac tttttggtct tgccagtttg cgttttcagg gtgaccaaca
     1021 tttgctggat attgccttct ggtgcaacga aaaaggggtc agcgcccgtc agcagcttag
     1081 cgttcagcaa caaaatggta tctggacatt agttcaaagc gaagaggcgg agatccaacc
     1141 acgcagcgac gaaaaacgca ttctgagtaa tgttgctgta ctggaaggtg cgccgccact
     1201 atcggaacac tggcaactgt tcaacaataa cgaagtcctg ttcaatgaag cccgtaccgc
     1261 tcaggcggcg acggtggtct tttctttaca gcaaaatgcg caaatcgagc cactggcccg
     1321 cagcattcat accctgcgtc gccagcgcgg tagtgcgatg aaaatcctcg tgcgggaaaa
     1381 taccgctagc ctgcgcgcca ccgatgaacg tttgttattg gcctgcggtg caaatatggt
     1441 tattccgtgg aatgcgccac tctcccgttg tctgacgatg atcgaaagcg tgcaagggca
     1501 gaagtttagt cgctatgtgc cggaagatat cactaccttg ctgtcaatga cccagccgct
     1561 caaactgcgt ggtttccaga agtgggatgt gttctgtaat gccgtcaaca acatgatgaa
     1621 taaccctcta ttacctgccc acggtaaagg cgttctggtt gccctacgtc cggtaccggg
     1681 tatccgcgtt gaacaagccc tgacgctgtg tcgccctaac cgtaccggcg atatcatgac
     1741 cattggcggt aatcggctgg tgctgtttct ctcattctgt cggattaacg atctggatac
     1801 cgcgttgaat catattttcc cattgcctac tggcgacatt ttctcaaacc gtatggtctg
     1861 gtttgaagat gatcaaatca gtgccgagct aatgcagatg cgcttgcttg ccccagaaca
     1921 atggggcatg ccgctgcctt taacgcaaag ttctaaaccg gtcatcaatg ccgagcacga
     1981 tggtcgccac tggcgacgaa taccagaacc catgcgactg ttagatgatg ctgtggagcg
     2041 ctcatcatga tgaccatcag cgatatcatt gaaattattg tcgtttgcgc actgatattt
     2101 ttcccgctgg gctatctggc gcggcactct ttgcgacgca ttcgcgacac cttacgtttg
     2161 ttctttgcta aacctcgtta tgttaaaccg gccgggacgt tacgccgcac ggaaaaagcc
     2221 agggcaacca aaaaatgact caatttacgc aaaataccgc catgccttct tccctctggc
     2281 aatactggcg cggcctttcc ggctggaact tctattttct ggttaagttc ggcctgttgt
     2341 gggcgggata tcttaacttc catccgctcc tcaatttggt gtttgccgcg tttctgctga
     2401 tgcccattcc gcgctacagc ctgcatcgct tgcgccactg gattgccctg ccgatcggct
     2461 ttgctttgtt ctggcatgac acctggttgc ctggcccgga aagcataatg agccagggtt
     2521 cgcaggtggc ggggttcagt accgattatt taatcgacct tgtcacacgc tttattaact
     2581 ggcagatgat tggggccatt tttgttttat tagtggcctg gttattcctg tcacaatgga
     2641 ttcgcattac cgtttttgtg gttgccatac tgctatggct gaacgtactt accctggcgg
     2701 gaccaagttt ctccttgtgg ccagccggac aaccgacgac cactgtaaca acgacgggtg
     2761 gtaacgcagc ggcaaccgtt gcggcgacgg gtggcgcacc ggtagtgagt gatatgcccg
     2821 cacaaactgc accgccaaca acggcgaacc ttaacgcctg gctgaataat ttctataacg
     2881 cggaggcaaa acgtaaatcg accttcccgt cttcgctgcc cgctgatgct cagccatttg
     2941 aactactggt gattaacatc tgttcgcttt cctggtcgga tatagaagcc gccgggttga
     3001 tgtcgcatcc actgtggtca catttcgata ttgagttcaa gaactttaac tccgccacct
     3061 cctacagtgg cccggcggcg atccgtttac tgcgcgccag ctgcgggcag acttcgcaca
     3121 ctaatctgta tcaaccggca aataacgact gctatctgtt tgataacctt tcgaaactgg
     3181 gctttaccca gcacctgatg atggggcata acggccagtt cggcggtttt ttgaaagaag
     3241 ttcgcgaaaa tggcggcatg cagactgaat tgatagatca aacaaatctg ccggttattt
     3301 tgctgggctt tgatggttcg ccggtttatg acgataccgc cgtgcttaac cgctggctgg
     3361 acgttaccga aaaagataaa aatagccgta gtgccacgtt ctacaacacg cttccactgc
     3421 atgacggcaa ccattatccg ggggtcagca aaacagcgga ttacaaagcg cgggcgcaga
     3481 aattctttga tgaactggac gccttcttta ctgaactgga gaaatcgggt cgtaaagtga
     3541 tggtggtcgt ggtgccggaa cacggcggcg cgctgaaggg cgacagaatg caggtatctg
     3601 gcctacgtga tatccctagc ccgtctatca cagacgtccc cgttggggtg aaattcttcg
     3661 gcatgaaggc accacatcag ggggcaccga ttgtcattga ccaaccgagc agcttcctgg
     3721 ctatctccga tctggtggtt cgcgttcttg atggcaagat tttcaccgaa gacaatgttg
     3781 actggaaaaa actcaccagt gggttgccac aaacagcacc ggtctccgag aactcaaatg
     3841 cagtagttat tcaataccag gataaaccgt acgttcgcct gaacggcggc gactgggtgc
     3901 cttacccgca gtaaacacga aaaaggccgc agagtttaac ctctgcggcc tgatccgggc
     3961 gcaaattgcc attacggcag cctgacgccc gcatggcacg ttacttccgc ttgttcagcc
     4021 agttcacgat catactggca agaataccag caatgaccgg agccgctaaa tcatgccaga
     4081 aggccatgcc cagttctgcg agcgtcatat agccgcctgt attgtaatga caacgtttcg
     4141 cggctattct tgtattgttt ggatgcaaga atggcccccg tggtgttgtc aggtgcatac
     4201 ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat taagcccccg
     4261 caacattgcg cctcaccgga atccttccgg cttgacgctg attctacgac tgtttttctg
     4321 ttccggaatc cgtctcatct tcactctgtg ctgcgcattc cgcagaatat tatccgcttg
     4381 caaccgttgc atttttctta cgaaaaaggc cgcagagttt cactcctgcg gcctggtccg
     4441 ggcgcaaatt gccattacgg cagcctgacg cccacatgac acgctacttc cgcttgttca
     4501 gccagttcac gatcatactg gcaagaatgc cagcaatgac cggagccgct aaatcatgcc
     4561 agaaggccat gcccagctct gcgagcgtca tatagccgcc tgtgttgtaa tgacaacgtt
     4621 tcgcggctat tcttgagtgg tctaggttca agattagccc ccgttatgtt gtcaggtata
     4681 tacctgcaac gtgcgggggt tttctctctc cagcaaccaa tgccaccagg gataaagccc
     4741 ccgcaacatt gcgcctcacc ggaacccttc cggcttgccg ctgattttac gattgctttt
     4801 tactgtcagc acccggcaat cttcctcatt gcgcgatgcc ttccagctta atttctttat
     4861 ttgcaatagt tgcagttcat atccgggctg actccagact ggtagtgaaa tgcgcaattt
     4921 tgtagaattg cgcctcctcg cgacctcccc agtgaatctg ttgcctcatt ttttacatac
     4981 tgcgtattcg acttctccac ctgttgcgca agagaaactg ggtttattca tttttgcgag
     5041 gccgacttct ttctggacag gactttatgc agcacaacac actatcgaaa cacaatcaga
     5101 aattgccgtt tacacgctac gacttcggct gggttttatt atgcataggc atggcgattg
     5161 gtgccggaac cgtgctgatg ccagtacaaa ttggcttgaa gggaatttgg gtatttatta
     5221 ccgcagcgat cattgcttat cctgccacct gggtagtgca ggacatttat ttaaaaaccc
     5281 tttctgaaag cgattcctgt aatgactaca ccgatattat cagtcattac ctggggaaga
     5341 actggggaat tttcctcggg gttatctact ttttgatgat tatccacggg atttttatct
     5401 actctctctc cgtggttttc gacagcgcct cgtacctgaa aaccttcggt ttaaccgatg
     5461 ccgatctttc acaatctcta ctttataaag tcgctatttt cgccgtactg gtggcgattg
     5521 cgtctggtgg tgaacgatta ctgtttaaga tttccgggcc aatggtggtg gtcaaagtag
     5581 ggattattgt cgtgttcggt tttgcgatga tcccgcactg gaatttcgcc aatataaccg
     5641 ccttcccgca agcctccgtc tttttccgcg atgtcctgct tacaattcca ttttgcttct
     5701 tttctgcggt atttattcag gtacttaacc caatgaatat tgcctatcgt aaacgggaag
     5761 cagataaagt actggcaacc cggctcgcgc tgcgtaccca ccgaattagt tatatcacgc
     5821 tcatcgcggt gatcctgttt tttgcctttt cgtttacctt ctcaattagc cacgaagaag
     5881 ccgtttctgc ctttgaacaa aatatctcgg cactggcgct ggccgcgcag gtgatccctg
     5941 gacatatcat tcatatcacc tctacggtgc tgaatatctt tgccgtactg accgcattct
     6001 ttggcattta tctcggtttc cacgaggcta ttaaaggcat tattctcaat ctgttaagcc
     6061 gaattattga taccaagaaa attaactcac gcatgctgac tctggcgatc tgcgctttta
     6121 tcgtcattac attgacgatt tgggtttcgt ttcgtgtatc ggtgctggtg ttctttcagt
     6181 tgggaagccc gttatatggc attgtgtcgt gcctcattcc gtttttcctg atctataaag
     6241 tcgcacaact ggaaaaactt cgcggattta aagcctggct aattctgctt tacggcattt
     6301 tgctatgctt gtcgccactg ttgaagctga ttgagtaaac cggagcgcat ggccccggtt
     6361 ttgtgagtta acgctgcgga ttttcatcct gatcaacagc aaaacaagcg accagttgac
     6421 cgccgtagtc ttttagctgc ggctgcaact gggtgcaggg gccgaagcgc cgacgacagc
     6481 gggcgttgaa ggcgcaaccc ggcggaggat tcagcgggct tggcagttca ccggtgagtt
     6541 tgatgcgttc acgacgatcg tccgggttca ggcgtggcgt cgcggagagc agcgcctggg
     6601 tgtacggatg gcgcgggtta ttgaagattt ggtccttcgt tcctttctcc acgcagcggc
     6661 ccaggtacat caccatcact tcatcagcaa tgtgctccac caccgacagg tcgtgggaga
     6721 taaagacata agaaagcccc aactcctgct gcaaatccat catcagattc agcacctgcg
     6781 cacgcactga gacgtccagc gcagaaaccg gttcatcggc aatcaccacg tccgggtcga
     6841 gcatcagacc acgggcaata gcgatacgct gacgttgtcc gccggagaac atatgcggat
     6901 agcggtcata gtgctcggtt ttcaggccga ctttcgccat catcgacagg gctttttccc
     6961 gacgctgttc tttgctgaag ctggtgttaa taagcagcgg ctcttcgaga atttgcccaa
     7021 cttttttacg cggattcagc gaaccgtaag ggttctggaa gacaatctgg attttctgcc
     7081 gacgcagctt ctgcgcctgc ggatcgtgct tgagcagatc ctgcccctgg taatacagct
     7141 caccaccggt gggcgtttcg atcatcgtca gcaaccgacc gagggtcgat ttaccgcagc
     7201 cagattcgcc cactactgcc agcgttttgc cacgttcaag gttaaacgaa acgccgtcca
     7261 gcgccttcac cagacgttcc ggcgcgaaca tgcctttctt caccggataa tgttttttca
     7321 ggtcgatagc ctgcaacagc ggttgttgcg aggtggcctc ttgcgtactc atagtgtcgg
     7381 cctcccggca tcatcaagtg ggtaatggca tttggactga cgcccatcag cgagcatatt
     7441 cagcgccggt tcttcagcgc gacatctgtc agtggcatag gggcagcgcg ggttaagcag
     7501 gcagccgttc gggcggtcgt acttaccggg aacgacacct ggcaacgacg ccagacgttc
     7561 tttgtcctga gcaaattctg gcagcgcacg cagcaatgcc tgagtatacg ggtgacgcgg
     7621 cgcatggaag atggcgtgcg catcaccggt ttccaccacc tggcctgcat acatcacgat
     7681 gattttatgt gccgcttccg ccaccagcgc caggtcatgg gtaattaaca ccagcgccat
     7741 gttctctttc tgctgtagct ccagcagtag ttcgatgatt tgcgcctgaa tggtcacgtc
     7801 cagcgcggtg gtcggttcat cggcaatcag cagttttggc cgacaggcaa tcgccatggc
     7861 gatcatcacg cgctggctca tgccgccgga aagctgatgc gggtaaatat ccagacgcga
     7921 tgccggatcg ggaataccga cctgattcag caggtcaatc gcccgctgac ggcgagtgct
     7981 cttattaccg ccctgatgca ccttaatcgc ttccatgatc tggaaaccca cggtgtagca
     8041 cgggttaagg ctggtcatcg ggtcctggaa gatcatcgcc acttcggcac ccaccaggtt
     8101 gcggcgctct ttttctgata tacgctgcaa atcctgaccg ttaaacacca gcttttccgc
     8161 cattacgcgg cccggataat caatcagccc cataatcgcc agtgaactga ccgacttacc
     8221 ggaaccagac tcacccacaa tcccgaccac ttcgccctgt tttacgctgt agctgatgcg
     8281 gtctacggcg cggaacggtg cgctttcgtc gccgaaatgc accgataatt tatctacatt
     8341 taataacgcc atctcgaacc tcttactgct tcagtttggg atcgagcgcg tcacgcagac
     8401 cgtcacccat caggttaaat gccagcaccg tcagcaggat cgccagaccc gggaaggtca
     8461 cgacccacca ggcgctttgc gcgaactgca acacgtcgga gagcatggtg ccccactctg
     8521 gtgttggcgg ctgcgctccc atgccgagga agccaagggc ggccatatcg agaatggcgt
     8581 tagagaaacc gagcgacgcc tgaacaatca gcggcgcaag gcagttcggg aagatgttaa
     8641 taaacatctg acgcatcgcc ccggcacccg ccacgcgaga cgcggtgacg taatcgcggt
     8701 taacttccac cagcacggcg gcgcgggtta agcgcacata gtgcggcaag gcaacgaagg
     8761 tcagtgccag cgcggcgtta ccaatcgacg ggccgaagat tgccaccagc accagcgcca
     8821 gcagcagact tggcagcgcc agcatgatat cgaccacgcg cataatgatg ttatcgacca
     8881 ggccgccaaa gtaaccggcg atcaggccga gaataacgcc cataatcagc gataacacaa
     8941 ctaccagaca gccaaccagc agcgacaggc gcgcaccgta catcaggcgc gacagcacat
     9001 cacggcctac gtcatcggtg cccagcaagt gcgccatgct gccgccttcc tgccaggctg
     9061 gcggggcgag cagtgcatcg cggaactgtt ccgccgggtt atagggtgca atccagttgg
     9121 caaagatcgc gatgaacagc acgatgacga cgtaaaccag cccgacgacc gcgcctttgt
     9181 tgcgtttaaa atagtgccag aactcctgta acggggtcat cggcaccggt gcgctaatca
     9241 ctttattttc agtaacctgt gacatgatgt ccccttactt cttatgacga atacgcgggt
     9301 tcaccacgcc gtacagcaga tcgaccagca agttgacgag gataatcatc gtcgccacca
     9361 gcaatacgcc gccctgcact accggatagt cgcggcgttg cagcgcgtca atcaaccagc
     9421 gccccagacc gggccacgag aagatggttt cggtcagaat cgcccccgcc agcaatgttc
     9481 ccacctgcag gccgataacg gtcactaccg gcagcatcgc gttacgcaac gcatggacga
     9541 taatcacccg catgcgggtt agccctttgg cgcgcgcggt gcggatgtaa tcctcgccca
     9601 gcacttccag catcgaggag cgtgtcatac gcacaatgac cgccagcgga atagtaccca
     9661 gcacaatggc aggcaagatc atatgggcga cggcatcgat aaagttgccg tcttcacccc
     9721 agatggcggt gtcgattagc ataaaaccgg ttaacggatt ggagtcatcg aggaacacca
     9781 tatcgctcac gcgaccggag acgggcgtca ggttccagtg caccgaaacc agcatgatca
     9841 gcatcatgcc ccaccagaag atcggcatcg agtagccggt cagcgccagg ccaaccgctg
     9901 tgtgatcgaa aatggaaccg cgtttaaccg cagccagcac gccgaccgga ataccgaccg
     9961 cagtcgcaaa aatcatcgcg cagacgccaa gttccagcgt ggcctggagg cgcggcacga
    10021 actcttccca aaccgggatg cggcttttca ttgaaatgcc tagatcgcca tgcataacgc
    10081 cccaaatgta atggagatac tgctgccaca tcggtttatc taagccgagt tcagccagca
    10141 gctgcgcgtg acgctctggg gagatcccac gttcgcccgc catgatcatc accggatcgc
    10201 ccgggatcat gtggacaaag gcaaatgtga gaagggtaat accgataaac gtggggatga
    10261 cgagtcccaa acgtcggaga ataaactgca acataacccg gattctctat agtgacccgc
    10321 agcctggaac tgcgtgtctg tattgctcac aaatcaattc ccgcccaggt cgggctggga
    10381 atgaagtatt gctgccggat ggcgctgcac gcagaggcac agcgccaccg ggtatggctt
    10441 ttaattattc gatagagacg ttttcgaagt gatgtttgcc taatggatca accacatagc
    10501 ctttaacttc tttgcgtacc ggttcaaaca cggtggagtg agcgatgatc agtgccggag
    10561 cctgatcgtg catcaccacc tgcgcttgct tgtacagttc aacgcgtttg ttatggtcgt
    10621 cggtagcacg cgccggttga atcagatctt caaacggttt gtagcaccat tttgagtagt
    10681 tggagccttg ttcagaggcg gcgcagctga acagggtggc gaagaagtta tccggatccc
    10741 cgttatcgcc agtccagccc atcattaccg tctggtgctc gccatctttc gcacgcttga
    10801 ggtactcacc ccattcgtag gtgacaattt tggcctgcac gccgactttc gcccagtcag
    10861 cctgaatcat ctccgccatg cggcgagcgt tcgggttata tgggcgctgt accggcatcg
    10921 cccacaggtc gatggagaaa cctttttcca gacccgcttc tttcagcaag gctttcgctt
    10981 tttcaggatc gtaggtgtaa tcctgaacgt cgtcgttata gccccacatg gttggcggga
    11041 tcaggttttt cgctgataca cccgcgccct gataaaccgc tttgatgagc gcgtctttgt
    11101 tcaccgcgta ggtcagtgcc tggcgaactt tcacgtcatc cagcggtttt ttctgcacgt
    11161 tataggagag gtagccgacg ttcagccccg gcatttccat caggttgatg gatttatcct
    11221 gcttcatgcg agcgatgtct gccgggttcg ggtacggcat cacctggcat tcattcttct
    11281 gcaatttcgc ataacgcacg gaagcgtcag gagtaataga gaagaccagc gtatcgatct
    11341 gcggtttggt gccccagtag ccatcaaacg ctttgtagcg gatacgggag tctttttggt
    11401 actgctgtaa ctggaacgga ccggtgccga ttgggttgag gtccagtttt tccggtgtac
    11461 cggctttcat catcgcgtca gcatattctt ttgacagaat agaggcgaag tccattgcca
    11521 ggtcagcgag gaacggcgct tccgggcgag tcagcacaaa ctgaacggtg ttgtcgtcca
    11581 cctttttcac ttcgctgatc agctccggca agcccatgcc ttcgaagtat tcgtagctgc
    11641 cgccagaaac tttatggtac gggttttgcg cgtttttctg acgatcaaac gagaacacca
    11701 cgtcatcggc gttcagttca cgcgtcggtt tgaattcttt attgtcgtgc cacttcacac
    11761 ctttacgcag atggaaggta taggttttac cgtcttcgct gacttcccac ttttcagcga
    11821 ggcccgggat cacttcagtg gtgccgattt taaattcaac caggcggtta taaagcggta
    11881 cggaagaggc gtcataggtg gtgccggagg taaacagctg cgggttaaac ccttccggag
    11941 atccttctga gcaataaacc agagttttag cctgaacact tgctgcgacg gtcatagcca
    12001 ccaggctgag accaagcttc agcatccctg actttttcaa ggaaatacgc attattctgc
    12061 tccaattgtg atgtttgttg ttttaaccct ttgcagtggg ttgtcgctgc ctgacctttt
    12121 ttgttttttg cccggtcggg tcaacgttat gaggtgggga tgccgtacta attaacatca
    12181 gtgtaacagt gcggatcctc cataaaatgc ccctcgtgac ctacaatctg tcaacagaat
    12241 gtgaaaacgt caatacagcc aaccgggatt tacaccaacg gtgagaatcc acaaacaaag
    12301 attaaaaaaa cttcaaacag ctatttgcag cagagaaatt tgtgctactc caacatgacc
    12361 agaacaatca gcttaatatt tagcaacatt tggtgattaa gttttatgca tttaatgaaa
    12421 aaaatctgag gaaaaggtgg atatctgagc gattaatacc atgaacgcta aaacgcacag
    12481 cggaaaatgc cagcgccagc cataagtaac gcaaaaaaag atttaagcaa atataaaaaa
    12541 agacaatggt ttctgtcaca aatccgttaa tggatagtga gatatggggc gcaaagttgg
    12601 caggaaaatg tgtggtgtgt tggtcgaata aggcggtcgc gccgcatccg acaataagtg
    12661 cggtgccgtt gcctgatgcg acgctggcgc gtcttatcag tatctacgtc gagtcgaacc
    12721 tccccgcccc cggaggttct catcctcatg agctacaaat gcaaaaaagc ctgctcgttg
    12781 agcaggcttt tcgaatttgg tcggtgatag aggattactc gccacttcgt ggctcgccct
    12841 gcgggccgtt gctggcgcaa cgt
//
LOCUS       DAZGTR010000122        12774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGTR010000122 DAZGTR010000000
VERSION     DAZGTR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.124"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            146..712
                     /gene="yqaB"
                     /locus_tag="ACHLCY_003877"
     CDS             146..712
                     /gene="yqaB"
                     /locus_tag="ACHLCY_003877"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP0371046.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            709..1137
                     /locus_tag="ACHLCY_003878"
     CDS             709..1137
                     /locus_tag="ACHLCY_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP0371047.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1210..2766
                     /gene="gshA"
                     /locus_tag="ACHLCY_003879"
     CDS             1210..2766
                     /gene="gshA"
                     /locus_tag="ACHLCY_003879"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP0371048.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            2916..3431
                     /gene="luxS"
                     /locus_tag="ACHLCY_003880"
     CDS             2916..3431
                     /gene="luxS"
                     /locus_tag="ACHLCY_003880"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP0371049.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3495..5033)
                     /gene="emrB"
                     /locus_tag="ACHLCY_003881"
     CDS             complement(3495..5033)
                     /gene="emrB"
                     /locus_tag="ACHLCY_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP0371050.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5050..6222)
                     /gene="emrA"
                     /locus_tag="ACHLCY_003882"
     CDS             complement(5050..6222)
                     /gene="emrA"
                     /locus_tag="ACHLCY_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP0371051.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6349..6879)
                     /gene="emrR"
                     /locus_tag="ACHLCY_003883"
     CDS             complement(6349..6879)
                     /gene="emrR"
                     /locus_tag="ACHLCY_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP0371052.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(6970..7305)
                     /gene="ygaH"
                     /locus_tag="ACHLCY_003884"
     CDS             complement(6970..7305)
                     /gene="ygaH"
                     /locus_tag="ACHLCY_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP0371053.1"
                     /translation="MSYEVLQLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7295..8032)
                     /gene="ygaZ"
                     /locus_tag="ACHLCY_003885"
     CDS             complement(7295..8032)
                     /gene="ygaZ"
                     /locus_tag="ACHLCY_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP0371054.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8156..9340)
                     /locus_tag="ACHLCY_003886"
     CDS             complement(8156..9340)
                     /locus_tag="ACHLCY_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0371055.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     LLGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFINDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9532..10524)
                     /gene="proX"
                     /locus_tag="ACHLCY_003887"
     CDS             complement(9532..10524)
                     /gene="proX"
                     /locus_tag="ACHLCY_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP0371056.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10582..11646)
                     /gene="proW"
                     /locus_tag="ACHLCY_003888"
     CDS             complement(10582..11646)
                     /gene="proW"
                     /locus_tag="ACHLCY_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP0371057.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIDAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11639..>12774)
                     /gene="proV"
                     /locus_tag="ACHLCY_003889"
     CDS             complement(11639..>12774)
                     /gene="proV"
                     /locus_tag="ACHLCY_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP0371058.1"
                     /translation="YIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGK
                     STMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLD
                     NTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINP
                     DILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNG
                     EVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSA
                     LKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLS
                     ELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3167 a         3296 c         3374 g         2937 t
ORIGIN      
        1 ttctcccaaa aatttccttc aaaaatatct catttctaaa taactacttt atccatttcc
       61 tgaattttct tctccttcgc catcagcgac aaaatcaagc aattacgcta aagtagcgtc
      121 atatttttcg acttgcgaga agaccatgta cgagcgttat gcaggtttaa tttttgatat
      181 ggatggcaca atcctggata cggagcctac gcaccgtaaa gcgtggcgcg aagtattagg
      241 gcactacggt cttcagtacg atgttcaggc aatgattgcg cttaatggat cgcccacctg
      301 gcgtattgct caggcaatta ttgagctgaa tcaggccgat ctcgacccgc atgcgttagc
      361 gcgtgaaaaa acagaagcag taagaagtat gctgctggat agtgtcgagc cgcttcctct
      421 tgttgaagtg gtgaaaagtt ggcatggtcg tcgcccaatg gctgtaggaa cggggagtga
      481 aagcgccatc gctgaggcat tgctggcgca cctgggatta cgccgttatt ttgacgccgt
      541 cgtcgctgct gatcacgtca aacaccataa acccgcgcca gacacatttt tgttgtgcgc
      601 gcagcgtatg ggcgtgcaac cgacgcagtg tgtggtcttt gaagatgccg atttcggtat
      661 tcaggcggcc cgtgcagcag gcatggacgc cgtggatgtt cgcttgctgt gagtgaagcg
      721 ttatcgcttt tctcgttgtt tgccagtagt tttctcagcg ctacactatt acccggcaac
      781 tccgaagtcg tgctggtggc aatgttgctt tccgggatca gttatccctg ggttttagtt
      841 ttaacagcaa caatgggtaa tagccttgga gggttaacta acgttatcct tgggcgtttc
      901 tttccattgc gtaaaacatc gcgctggcaa gagaaagcta ccggctggct gaaacgctat
      961 ggtgcagtca cgctattatt aagctggatg cccgtggttg gcgatttact gtgtctgtta
     1021 gcgggatgga tgcgcatctc ctgggggcca gtaatctttt ttttgtgcct tggtaaagcg
     1081 ttacgctatg ttgcagttgc agcagcgacc gttcagggca tgatgtggtg gcactaattg
     1141 taggcctgca catatggtca ccattacagt tatgctaatt aaaacgattt tgacaggcgg
     1201 gaggtcaatt tgatcccgga cgtatcacag gcgctggcct ggctggaaaa acatcctcag
     1261 gcgttaaagg ggatacagcg tgggctggag cgcgaaactt tgcgtgttaa tgctgatggc
     1321 acactggcaa caacaggtca tcctgaagca ttaggttccg cactgacgca caaatggatt
     1381 actaccgatt ttgcggaagc attgctggaa ttcattacac cagtggatgg tgatattgaa
     1441 catatgttga cctttatgcg cgatctgcat cgttatacgg cgcgcaatat gggcgatgag
     1501 cggatgtggc cgttaagtat gccatgctac atcgcagaag gtcaggacat cgaactggca
     1561 cagtacggca cttctaacac cggacgcttt aaaacgctgt atcgtgaagg gctgaaaaat
     1621 cgctacggcg cgctgatgca aaccatttcc ggcgtgcact acaatttctc tttgccaatg
     1681 gcattctggc aagcgaagtg cggtgatatc tcgggcgctg atgccaaaga gaaaatttct
     1741 gcgggctatt tccgcgttat ccgcaattac tatcgtttcg gttgggtcat tccttatctg
     1801 tttggtgcat ctccggcgat ttgttcttct ttcctgcaag gaaaaccaac gtcgctgccg
     1861 tttgagaaaa ccgagtgcgg tatgtattac ctgccgtatg cgacctctct tcgtttgagc
     1921 gatctcggct ataccaataa atcgcaaagc aatcttggta ttaccttcaa cgatctttac
     1981 gagtacgtag cgggccttaa acaggcaatc aaaacgccat cggaagagta cgcgaagatt
     2041 ggtattgaga aagacggtaa gaggctgcaa atcaatagca acgtgctgca gattgaaaac
     2101 gaactgtacg cgccgattcg tccaaaacgc gttacccgca gcggcgagtc accttctgat
     2161 gcgctgttac gtggcggcat tgaatatatt gaagtgcgtt cgctggacat caacccgttc
     2221 tcgccgattg gtgtagatga acagcaggtg cgattcctcg acctgtttat ggtctggtgt
     2281 gcgctggctg atgcaccgga aatgagcagt agcgaacttg cctgtacgcg cgttaactgg
     2341 aaccgggtga tccttgaagg tcgcaaaccg ggcctgacgc tgggtatcgg ctgcgaaacc
     2401 gcgcagttcc cgttactgca ggtgggtaaa gatctgttcc gcgatctgaa acgcgtcgcg
     2461 caaacgctgg atagcattaa cggcggcgaa gcgtatcaga aagtgtgtga tgaactggtt
     2521 gcctgcttcg ataatcccga tctgactttc tctgcccgta tcttaaggtc tatgattgat
     2581 actggtattg gcggaacagg caaagcgttt gcagaagcct accgtaatct gctgcgtgaa
     2641 gagccgctgg aaattctgcg cgaagaggat tttgtagccg agcgcgaggc atctgaacgc
     2701 cgtcagcagg aaatggaaac cgctgatacc gaaccgtttg cggtgtggct ggaaaaacac
     2761 gcctgacaga aaagaaaaag gccactcgtg agtggccaaa atttcatctc tgaattcagg
     2821 gatgatgata acaaatgcgc gtctttcata tactcagact cgcctgggaa gaaagagttc
     2881 agaaaatttt aaaaaaaatt accggaggtg gctaaatgcc gttgttagat agcttcacag
     2941 tcgatcatac ccggatggaa gcgcctgcag ttcgggtggc gaaaacaatg aacaccccgc
     3001 atggcgacgc aatcaccgtg ttcgatctgc gcttctgcgt gccgaacaaa gaagtgatgc
     3061 cagaaagagg gatccatacc ctggagcacc tgtttgctgg ttttatgcgt aaccatctta
     3121 acggtaaggg tgtagagatt atcgatatct cgccaatggg ctgccgcacc ggtttttata
     3181 tgagtctgat tggtacgcca gatgagcagc gtgttgctga tgcctggaaa gcggcaatgg
     3241 aagacgtgct gaaagtgcag gatcagaatc agatcccgga actgaacgtc taccagtgtg
     3301 gcacttacca gatgcactcg ttgcaggaag cgcaggatat tgcgcgtagc attctggaac
     3361 gtgacgtacg catcaacagc aacgaagaac tggcactgcc gaaagagaag ttgcaggaac
     3421 tgcacatcta gtcagtaaac tatcttcaca attaattgaa aaaagccagt tcaaatgaac
     3481 tggcttagtt gtacttagtg cgcaccgcct ccgccgccac ctgcgccaaa tggcggttta
     3541 gcaaaccaca ccagccccag cagtacgagg aatattccgg ctgacatcca gaagatctca
     3601 ttggcggaaa taatcagccc ctgattggtg atctgctggg caatccagcc tgatgcctgc
     3661 tgttgcgtca tcccaagccc ttccagttga ctgtacatcg cctgggcatt cgggttgaac
     3721 gggtttaccg actcagtcaa ctgcgcatgg tgcatcgact cgcggttggt ccacatggtc
     3781 gtggttatcg acgtgccgat agaccccgcc agcgttcgcg taaagttaga gaggctcgat
     3841 gccgccgcca gtcgttccgg tggcaaacca gacagcgtaa tggtggtcag cggcataaag
     3901 aagcaggcca ccgcaaaccc ctggataaac tgcggccagg ccgacgcgcc aaaatccata
     3961 cctggttcaa aggtataggc acgccagtag aagcagacgg catacataat aaagctgaag
     4021 gttaccagcc gccgcatatc cagtttatgc gcgaagcggc cgataatcgg cgacaggatc
     4081 accggaataa tccctaccgg cgcagaggcc aaacctgccc aggtcgccgt gtaaccgtag
     4141 acctcctgca acaactgcgg cagcagaaca atagcgccga agtagagcat atacgcgagg
     4201 ctgatacaca agcagccgat ggtgaagttg cgcgacttaa acaacgacag atcgactatc
     4261 gggttatcgt cggtcagctc ccagacaatc aggaagcaga tagccaccac cgccaccacg
     4321 gtaaggatga taatttcctg tgatgaaaac caatccagct ctttaccgcg gtcaagcata
     4381 atctgcaggc tgccgatacc aataaccagc agtgccagcc ccacggcatc aatccgccgc
     4441 cgttcggtgc gggtttcacg tccgcgcaga gtttgcagtg tcatcaacac caccgccacg
     4501 ccaatcggca cgttgatgaa gaatatccag ccccagtggt aattatcgct gatataaccg
     4561 ccgaggatcg ggccgcaaat tggcgcgaca atcaccgtca tcgaccacaa cgccagcgcg
     4621 atcgagcgtt tggctggcgg gtagttattc agcaatagac tttgcgaaag cgggatcaac
     4681 ggcccggcga caatcccctg aatcacgcgg aagaagatca gcatattcag gctgctggag
     4741 acaccacacg cccacgacgc aatagcaaag gcgatggtgg accaaaggaa cagtttcact
     4801 tccccgacgc gctttgccag ccagccggta agcgggatcg agatggcatt cgccaccccg
     4861 aaagaagtga ttacccacgt tccctggctg agcgatgagc ccagattccc ggcgatagtg
     4921 gggatcgcca cgttagcaat ggtggagtcc agcacctgca tgaatgtcgc cagtgacagc
     4981 gcaatcgtca taatgaccag ttgcgcgcct tccagcggtt tttgctgttg catcacacgc
     5041 acctctggat tagccagcgt tagcttttac gatatcgtcg atcagtttat tgacaggtgc
     5101 caggctgatt tcacgcgcgg tacttaccgc gaccggtgtt gaacgtactt tatttgccag
     5161 tacctgaccg tcacggttag tggtattgac gctcaccagc gtggacaaac caatgcgcag
     5221 cgggtattgc tccagctgtt tctggtccag ttcgatacgc acaggtagac gctgaacgac
     5281 tttgatccag ttaccggtcg cattttgcgc cggaagcagt gagaatgcgc tacctgtgcc
     5341 catatccaga ccaaccactt taccggtgta tttcacatca tcgccgtaaa tatccgtggt
     5401 gatagtgacc ggctgaccga tacgcatatt ggcaatctgc gtctctttaa agttggcatc
     5461 cacccacata ttggtggctg gaacgaccgc catcagcggc gtcgttgggc taatttgcgc
     5521 cccaggctgt accgcgcggc gggagacata accggtcatc ggactgacaa tacgggtacg
     5581 ctccagcgcc agccaggcgt tacgtacttc ggtggcagcc tgttgcacgg caggctgatc
     5641 ttccagttta gtccccagaa tcatcgcctg attggcattg tattgttgaa tagcgacgtc
     5701 cagttgcgcc tgggcactgg tgacggcgtc gcgggcgtgt tgcagctctt cgcgaccaat
     5761 caggttggca ttgcccagcg gcacacggcg gttgtagtcg ctttgtgctt tcgcgagggc
     5821 gattttttgc acctcaatat tcgcctgcaa ctgtttgctg ttaatcatca gctggtgggt
     5881 ttggcgaacg ctggaagcca gtgcagtttt ggctttttca aacgcctggc gagcatctgt
     5941 cgggtcgaga gtgaccagaa catcgccttc ttttacaaaa tcggtgttat cggcccagac
     6001 tttcgtcacg ctgccagaca cctgagacat aatttgaatc tgattccctg ccacgtatgc
     6061 gtcatcggtt tcttcgaaat gacgcagtac caaaaaccaa taaatcccta tcgctacggc
     6121 aataattata aagagcaagg tgagaaggag gagcagacgc ttacgtttgc cgctcttctt
     6181 taccggttgc tgcggggttt gagtctccgc atttgcgctc atattgttct ccacgatctt
     6241 cttaactcat cggctgagcc gagctgcttg ccaaaaaggc cagcatgttg cgatgctggc
     6301 cggtcatttt ttcttttata aatctggatt tttgagcgag atgacgcgtt agctcatcgc
     6361 ttcgagaacc acaccgtctt gttccatctg gtcgagacgg gagagcaatt tgcgggtgat
     6421 ttgctcgagc tgatcttttt ctgttgtgct gagcgcggac cagagttgat gcaggcagtt
     6481 atgctgcggt ggtaaaacct cgcgcaaaaa ctcgtgacct ttttccgtta attgcagatg
     6541 caggcagcgg cgatcgttat cgctttcacg acgttcgatc caaccgcgtt tttccagttc
     6601 atcggcaata cgcgtcgcgt tggtacggga tgatccaaga gcacaactta attcagaagg
     6661 ctgaatactg tggttttcct gagactccag cgtaatcaac gccataaaca acgtctcgtt
     6721 aatcccctga gccttcagca ttttattgcg gttctccagc agcttgcttt gcatgtgcat
     6781 gcaaagacga gtcagaagga tctcctgata aggaaaatct tcgtggcggc tggcgcgaaa
     6841 ttttagcatt tgttcaatgg gcgtaaacga actatccatt tgggtatgac ctcattaatt
     6901 tcagccgata tagtaacgat agtgacaaat aaagtaaatg tattgtttta gaaaaatgat
     6961 tcttgtgggt tatataatcg ccatcacttt ccaggcgagc ccataggcca gcgcactaag
     7021 cagtgttggg atgataatgc tgcgtgtttt atagaaactg gcacccagta ccgcgaagcc
     7081 gaccagcgtg ggcacgaaac ggcgtgtatc gtgcatcact tctggtgcgg tagagacaac
     7141 cagcagagcg catatcgagg cgatgccaat ggtgtcgagc aaaataccta ccgcgccacg
     7201 tttggttggg cgggcattac ccacacgcag gcgcagcggc aaatagcgga agcaataatt
     7261 cgccacgcca accagtaacc caagctgcag aacctcatag ctcatcgggc gctccttgcc
     7321 agaatgcctg gattaacgca gtgaggcagc cacagacaat gcctgccaga atggcgacgg
     7381 gaatagaaaa tagcgttacg cctgcaaggg caccaactaa cgctgcggta acgcaaagag
     7441 attgtttgcg ctggaaagag gcgagcagga aactcataaa gagtgccgga agcataaaac
     7501 ctaatgcagc ttcaacggcg ggataacctt gcagcaagcc gctgccggag aatgccccta
     7561 ttaccgtacc aaatacccac gatgaccatg aactgaaggc aatgccgatc atccagttct
     7621 cgctccagcg gcgattattg cgtaccagtt ttgcggttgc ggcggcaaaa acctcatccg
     7681 tcaggccaaa tgcccacagg gcggttttcg atttttgcag acgctgaata atacggctac
     7741 gcagtgacgg gccatacaac acatggcgaa catccattgc catgacggtc agtgcagcaa
     7801 cccacaaact actcccggct gccagcatcg cggtaatgac gaactggctc gcgcctgcat
     7861 aaatgatgca ggagaaaaaa acgctttcga gaggagagaa tcccagacgg gtcgcattca
     7921 gaccgaacgc aaaggccacc ggaatataac taataacaat cggtaaactg tctttgcatc
     7981 cttccatgaa ggtcgccgaa ccaggagcag gctgtggagt agggctttcc atagagtgta
     8041 cgcttaacga ttgttaatat tataaataga ctgaatgaat atcttaacct tatcagactg
     8101 atgggcttct taacaccctt ataagtgtaa agccacgaaa acggttgctg attgcttaat
     8161 ttgcggcttc ctgacgatga aaacctcgcc accagaccag taagttaacc agggcaatcg
     8221 tcgcgccagc cagacaaacg ccagcccaac cgccatgttg ccaggctgag gctgaaatta
     8281 gcgaaccggc ggcaccgcca ataaagtagc tggtcatgta acctgcggtc aggcgattac
     8341 gcgcatcagg gtgtattcga taaattaccg tctggttagt gatatgcacg ccctgtacgg
     8401 tgagatccag caccaggatt ccgataatca acgccagtac ggaagtgtga ccaaaccaga
     8461 tcgccagcca tgaaagtaat agcagcagca gaccgaaagt tgtggtgtgg tgcgatttac
     8521 ccttatcggc aaaaccgccc gccggacgag cgcccaacgc gccggcagct cccgcaagtc
     8581 caaacagacc aatgacacca tcgctgtagt taaaaggtgg agcggcaagc aaaaaggcca
     8641 ttgaggtcca gagaatgctg aagttggcaa aggtcaggca gccaagcaat gcgcgggtgc
     8701 gcaggatctt atcgttgata aacatgctga aaacggaacc caacaactgt gggtagttga
     8761 ggtgggtttc tgatttcatt tgtggcagac cacgccataa tgctagcgcc atcagagcca
     8821 ttaacaccga ggcaacccaa aagacggtgc gccagccgcc gaggtttgcc agtaacccgg
     8881 caacggttcg tgccagcaat atccccaaca gcagaccgct cataatggtg ccaaccactt
     8941 tgccgcgttt atccggtgaa gccagcgtcg ctgccagcgg aaccagaatt tgtgccacga
     9001 cagagaataa accggttaat gccgtaccga ggagcatcat cgccagcgac tgactgctgg
     9061 cggtaatcaa cataccgccc gctgccagta aggtcatcga gacaatcagg cggcggcgtt
     9121 caaacatatc accgagggga acaagaaaca gcagacctgc tgcatagccc aattgcgcgg
     9181 cggtaacaat aaagcctgcc gaactggcgg aaagggaaaa gttacgcgcg atggtgtcga
     9241 gtagcggctg ggcgtaatag ttactggcga ccgccagacc ggtggcgata gacatcagca
     9301 cgatcagcgc cgggctaagc tcatgattag gtttagtcat tatttttgtt catcaggtga
     9361 tgattaagct ggaaatcaat aatagcggat gttgatgaat atgggatgaa agatagcttg
     9421 ttgaaatgtg cgatgtggtt tgtaggcatg ataagacgcg tcagcgtcgc atcaggcatt
     9481 gtgtacagag cggcggatga ggcaaaaacg ccttatccgc ccgaataaaa attacttctg
     9541 cgctgccagc gcctcattca cccagccatc gaactgctgc tggtgggctt tgatccagcc
     9601 atcaacatgg ccctgaatat cgccttctga ggctttgccg tcatgcataa tggcgttctg
     9661 ggcgttaata tctgccactg gcaactgcat aatggcaaac agtttcgctg ctgccgggtt
     9721 tttctcggcc caggctttgt tggcaacgat atgcatggtg ctgaccggga agccataatt
     9781 cgcaccattc ggcagtttgg tatcggcgtt tttatcgccc ggcagtgcgg agaacggcac
     9841 ctgcaaccag accacatctt tccctggctt cagctcatta ctcacccagt acggcgtcca
     9901 ggtgtagtaa aacaccggtt tgccctcttt gtagcgactg atggtgtcgg ccatcatcgc
     9961 cgcgtagttc ccctgattat gcgtcacggt atgggtcagt tcatacgcgg caagctggtg
    10021 gttgatcgca ccttcgcagc cccagccagg gttacaaccg gttaaatccg cttttccgtc
    10081 gccgttggta tcgaacagtt tggcgatctt cggatctttc agttgtgcga tgttggtgat
    10141 tttgtactgg tcggcggttt tcttatcgat caggtaaccc tgtgccgcgc cgttaacaaa
    10201 taccccttca cgataaaatt tcttatcgcc accggcagct tcgtacatgt tgtcatgcag
    10261 tggcgtccag ttcacggcgg tgaaggttgc atcgccggaa gcaagcgagg tgtagccaac
    10321 gttgtaatcc acttcgctgg gtttgttgac ggtataacct aatttctcca gcgcacgact
    10381 gaccagcagc gtctggaagg tttcttcagt gatggtgctc tgaactggat taacagtaat
    10441 gcctttgccc ggcagatcgg cagcaaaagt ttgtgtagag ataagcgtgg caaacgctgt
    10501 cgcaaaaagt acgctatgtc gcattgttat tccttttttt ggcagggtga taatgcccgg
    10561 cgtcaccggg caagtgcaga gttacttaat gaatgggcgg gtcagcagac caacagggcc
    10621 agtggtgtac cagcgacggt tgccgcgact gcgtgagtcg cgcccaacgg cctgcgtcag
    10681 gcgatcgagg ataatggcga ggatcacaat cccgacgccg ccaacggtgg caagccccat
    10741 atccagacga ccgataccgc gaagtaccat ctgacccagc ccgccgacgg caatcatcga
    10801 ggcaatgacc accatagaaa gggccagcat cagcgtctgg ttaacgcccg ccataatggt
    10861 cggcatcgcc agcggtaact gaactttgaa cagcatctgg cgcgggctgg caccgaatga
    10921 gcgcgaggct tcaatcagat ccgccggaac ctggttaatc cccagaatgg tcagacggat
    10981 aatcggcggc agagcaaaga tgatcgtcac caccacgccc ggcacgttac cgataccaaa
    11041 tagcatgacg attggcacca gataaacaaa cgctggcgtg gtctgcatgg catcaagcag
    11101 tggacgaata attttcgccg ctcgcggact tctcgccagc cagatcccca gcggcaaacc
    11161 aatgacgata cagaacagca gggcggttaa caccagcgcc agggtaacca tggcctgcga
    11221 ccaggcaccg attgcgtcga tggcaatcag cgaaaccagc gtcgccacgc ccattccgac
    11281 cccggaaatc tgccaggcga tgagagcgaa aacgataatc gccaccggtg cgggcatacc
    11341 cagcagcaat tgctggaaac cgttgagaat gtaatcaacc ggaacgcgca cgccctggaa
    11401 gacgggacgg aaatgggtaa caacccagtc gatcccttca gtgacccaac tgtcgagcgg
    11461 gatcagcgtt ttatggaacg gatcgagaat attaaaatgc tcgacgtttg gcgcaggcgt
    11521 actggtcagc cagtcagcac caccgccgtc agtcggtgca gtcgccggtg taccccaggc
    11581 gtctgcggat tgcacggcac tgtccgccgc tggcgtggta tcccacggat tattttgatc
    11641 agccattatt taccccctca cgatctaaag cgcgcagcag cattcctttc gaaatgatgc
    11701 cgacatactg ttggtcctcg tcgaccacgg gcaccgcaca gggtgcctgt ccgacatgag
    11761 agagcaactc gctaagaggc gtttgtgcat cgactgctaa cggcgcatca atcagcgccg
    11821 catcaagacc ttgctgctgc gttaacgcgg ttttaagcga atcgatggaa actgcgccga
    11881 caaacttatt accgcgttcg ataacgtagc catattcgcg atcttcatcc tgcaataatt
    11941 tcagtgccga acgtgggccg aagccagggg ttttacgaat taagccattc ggtgtccggc
    12001 gggcaatatc tttcgcactg aatacctgac taatatcaac gccacggaag aaggtacgga
    12061 cataatcatt cgccggatta ttgagaattt catccggtgt gccgacctgt accacttcac
    12121 cattttgcat aatggcaatt cggtcgccaa tacgcatagc ctcatcgaga tcgtgggaaa
    12181 taaagacaat ggtgcgctga tgtttcgcct gtaattttac cagctcatcc tgcatctcgg
    12241 tgcgaattaa tggatcgagc gccgagaagg cttcgtccat taataatata tccggattaa
    12301 tcgctaacgc gcgggctaat cccacacgtt gacgcatccc gccagagagt tcatccgggt
    12361 agctgtgggc ataattttcc agcccgacct gacgcagtgc atcaagggct ttttcccggc
    12421 gttcttcggc attaattccg gccaattcca taccgaacgc agtattgtcc agcacggtca
    12481 tatgcggcat taaggcaaag gactggaaga ccatcgcaat cttttttctg cgcacctcac
    12541 ggagttcggc atcggatatt ttggcaatat ccacaccatc aatcagcact tgcccgcggg
    12601 tgggttcaat caggcgattg agaaggcgta ccattgtgga tttacccgag ccggataatc
    12661 ccatgatgac aaatatctcg ccttcttcaa tggccagact ggcgtctttt acgccaagcg
    12721 atagcccagt tttttccaga atttgttctt tcgaaagtcc ttgttcgata tatt
//
LOCUS       DAZGTR010000123        12744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGTR010000123 DAZGTR010000000
VERSION     DAZGTR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.125"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..834
                     /locus_tag="ACHLCY_003890"
     CDS             <1..834
                     /locus_tag="ACHLCY_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0371059.1"
                     /translation="IDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIV
                     AGNKNELLLLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAAN
                     NQLKYINLDFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSG
                     KNNCPTMQLMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPY
                     NIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWP
                     F"
     gene            1158..2546
                     /gene="narU"
                     /locus_tag="ACHLCY_003891"
     CDS             1158..2546
                     /gene="narU"
                     /locus_tag="ACHLCY_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP0371060.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2628..6368
                     /gene="narZ"
                     /locus_tag="ACHLCY_003892"
     CDS             2628..6368
                     /gene="narZ"
                     /locus_tag="ACHLCY_003892"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP0371061.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLDNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6365..7909
                     /gene="narH"
                     /locus_tag="ACHLCY_003893"
     CDS             6365..7909
                     /gene="narH"
                     /locus_tag="ACHLCY_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0371062.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7909..8604
                     /gene="narW"
                     /locus_tag="ACHLCY_003894"
     CDS             7909..8604
                     /gene="narW"
                     /locus_tag="ACHLCY_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0371063.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8601..9281
                     /gene="narI"
                     /locus_tag="ACHLCY_003895"
     CDS             8601..9281
                     /gene="narI"
                     /locus_tag="ACHLCY_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0371064.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9360..10253
                     /locus_tag="ACHLCY_003896"
     CDS             9360..10253
                     /locus_tag="ACHLCY_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP0371065.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINVLHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(10349..11194)
                     /gene="nhoA"
                     /locus_tag="ACHLCY_003897"
     CDS             complement(10349..11194)
                     /gene="nhoA"
                     /locus_tag="ACHLCY_003897"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP0371066.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11367..11936
                     /locus_tag="ACHLCY_003898"
     CDS             11367..11936
                     /locus_tag="ACHLCY_003898"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP0371067.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11940..12167)
                     /gene="pptA"
                     /locus_tag="ACHLCY_003899"
     CDS             complement(11940..12167)
                     /gene="pptA"
                     /locus_tag="ACHLCY_003899"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP0371068.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(12266..>12744)
                     /locus_tag="ACHLCY_003900"
     CDS             complement(12266..>12744)
                     /locus_tag="ACHLCY_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0371069.1"
                     /translation="DSYAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAV
                     SFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDC
                     KIRKGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGRRLS"
BASE COUNT         3105 a         3244 c         3223 g         3172 t
ORIGIN      
        1 attgatatag ataggctttc atcagttact tattttagtg cggcacataa tcaactagag
       61 tttgtgcaat tagaatcttg cgaatggctg caatacctga atctcagcca taatcaatta
      121 actgatattg ttgcaggtaa taaaaatgaa ctcttactgc tggatctatc ccataataaa
      181 ctaacaagtt tacacaatga cttatttccc aacttgaata cgttacttat taacaacaat
      241 ttgctttctg aaattaaaat attctatagc aacttctgca atgttcagac attaaacgct
      301 gctaacaacc agttgaaata tataaatctt gatttcctga cttatcttcc atctatcaaa
      361 agtttaagac tggacaataa taaaataacc cacattgata ctaataatac atccgatatt
      421 ggaactttat tccccataat aaaacagagc aaaaacttaa attttttaaa tgtttctggg
      481 aagaacaatt gccctactat gcagctcatg ttatttaatt tgttttcccc agcacttaag
      541 cttaatactg gcctggcaat tctttcgcct ggtgcatttg aagttcactc tgacggaata
      601 gatgtggata acgaattgtt tcactatcct attaaaaaag catatacccc atataatata
      661 cacacttaca agacagagga agttgtaaac cagaggaata taaaagttaa aaacatgacc
      721 ttagatgaaa taaacaatac ttactgtaat aacgattatt acaatcaggc aataagagag
      781 gaaccgatag accttctgga cagatcgttt tcctccagtt catggccttt ttagtgaata
      841 aatcatcact aattctataa gtgtctggtc ttgttgataa tatagttaat atgtaaccct
      901 ttagattcac gatctgaagg gttgaaacat gacatacgtt caaagattaa cccccgtttg
      961 aaggttagct tgttagttac atttagtaac acatatctgg ctacttaatc cccattctgc
     1021 ccccgcggtt acgctttctt atctattctt tcaatcagca cttcacccaa ttcatcctta
     1081 attctaataa ctggctattg cgccttacct cctgttttgt cttttttcat taccaatgtg
     1141 tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg
     1201 actggaaacc agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa
     1261 acctctggat atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg
     1321 cagttaccgt taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat
     1381 taaccgcatt accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc
     1441 ctatattcgg tggacgccga tggacggttt ttagtactgc aatcctgatt attccttgcg
     1501 tctggctcgg aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg
     1561 ctttgctatg cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct
     1621 tctttccaaa agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag
     1681 gtgtaagtgt aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc
     1741 tcggcgtcaa tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat
     1801 ggatttgggt accgctactg gcgattgcca cgatcgccgc atggtcaggg atgaatgata
     1861 tcgccagttc acgcgcctca attgccgacc agctccctgt cttacaacgc ctgcatctct
     1921 ggctgctgag cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt
     1981 ttgccatgct ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg
     2041 gcccatttat cggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aagttcggcg
     2101 gcgtgcgggt gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc
     2161 ttaccttacc gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc
     2221 tgtttctgac tgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc
     2281 gccagataac catttatcgg gtaaagatga aaggcggtag tgatgagcaa gctcagaaag
     2341 aagccgtcac cgaaacggcg gcggctctgg gctttatctc agccattggc gcagtgggcg
     2401 gcttttttat tccgcaggcg tttggcatgt cgctcaatat gaccggctct ccggtcggcg
     2461 cgatgaaagt gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg
     2521 gtcggcggaa gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc
     2581 cgacaaggca tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt
     2641 tggatcgctt tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg
     2701 tgatgcatag caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa
     2761 tcgtgcgttc cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta
     2821 aaaatggtct ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc
     2881 tgcccaatca tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctttaca
     2941 gcgctaaccg cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgcgaag
     3001 ccctcaagca acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa
     3061 agtgcctgag ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg
     3121 aactaaacca gctgattgcc gccgctaacg tctggaccat caaaacctac ggcccggatc
     3181 gcgttgccgg tttctcgccg atcccggcga tgtcgatggt ttcttacgcc gccggaacgc
     3241 gttatctgtc gctgcttggc ggcacctgtt taagtttcta cgactggtat tgcgacctgc
     3301 cgcccgcctc gccgatgacc tggggcgagc aaaccgacgt accggaatct gccgactggt
     3361 ataactccag ctacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg
     3421 cccacttctt taccgaagta cgctacaaag gcactaaaac catcgccatt acccctgact
     3481 actctgaagt ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg
     3541 ccctggcgat ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg
     3601 actactttat caactactgc cgccgctaca gcgacatgcc gatgctggta atgctggagc
     3661 ctcgcgacga tggtagctac gttcccgggc gcatgatccg cgcatctgac ctggtggatg
     3721 gactgggcga aagcaacaat ccgcagtgga aaaccgtagc agttaatacc gcaggtgaat
     3781 tggtagtgcc gaacggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg
     3841 aatccattgc cgccggtacg gaaaccgaat tgtcgttaac cctgctcggt caacatgacg
     3901 ctgttgcagg cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg
     3961 taaaacacaa tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg
     4021 gcaacacctg tccggtggtc agcgtttatg atttggtact ggcgaattac ggcctcgatc
     4081 gcgggctgga agatgaaaac agtgcgaaag attacgctga aatcaaaccg tacaccccag
     4141 cctggggtga gcaaattacc ggcgtgccgc gccagtatat tgaaaccatc gcccgtgaat
     4201 ttgccgatac cgcccataaa acgcatgggc gctcgatgat tatcctcggc gcaggtgtta
     4261 accactggta tcacatggac atgaactacc gcgggatgat caatatgctg atcttctgcg
     4321 gttgtgtcgg gcaaagcggt ggcggctggg cacactatgt cggccaggaa aaactgcgcc
     4381 cacaaaccgg ctggttgccg ctggcctttg cgctcgactg gaaccgacca ccacgccaga
     4441 tgaacagtac ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg
     4501 ctcaggagtt actttcaccg ctcgccgatg ccagtaagta cagcggtcat ctgattgatt
     4561 tcaacgttcg cgccgaacgt atgggctggc taccttctgc gccgcagctg gggcgtaacc
     4621 cgctcgggat taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc
     4681 aggcgctgaa atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc
     4741 atccgcgtaa tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg
     4801 agtatatgca gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg
     4861 ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc
     4921 tcgacctgct ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg
     4981 ttctgcccac cgccacctgg tacgaaaaag acgatatgaa cacctcggat atgcatccgt
     5041 ttattcatcc gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa
     5101 tctacaaagg tatcgccaaa gcattttcgc aagtgtgcgt gggtcatctt ggcaaagaaa
     5161 ccgacgtggt attacaaccc ctactgcatg actctccggc agagctctca cagccgtgtg
     5221 aagtgctcga ctggcgcaaa ggcgaatgcg atctgattcc aggcaaaacc gcgccgaata
     5281 ttgtggcggt ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat
     5341 tgatggacaa acttgacaat ggcggtaaag ggatttcgtg gaatacgcag gatgaaatcg
     5401 atttcctcgg caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga
     5461 ttgacaccgc cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc
     5521 atgttgcagt caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc
     5581 tggcgctgca caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta
     5641 aaattatctc cagccccacc tggtccggtc tggaaagcga tcacgtatcc tataacgcgg
     5701 gatacaccaa cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct
     5761 atcaggatca tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta
     5821 tcgacacccg tagcgtcagt gagatgcgcc agataccgcc aaacggcttc ccggaaaaag
     5881 cacttaactt cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc
     5941 tgctaatgct gacgctctct cgcggtggac cgattgtctg gatcagcgaa acagatgccc
     6001 gagaactgac cattgtcgat aacgactggg tggaagtatt caacgccaac ggcgcgctga
     6061 ctgcccgcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     6121 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     6181 actcggtcac ccgcgtttgc ccgaaaccaa cgcatatgat tggcggttac gcgcagctgg
     6241 cctggggctt taactactac ggcaccgtcg gatcgaaccg cgatgagttc atcatgatcc
     6301 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     6361 aaaaatgaaa atccgttcac aagtcggcat ggtgcttaac ctcgacaaat gtatcggctg
     6421 ccatacctgt tcggtgacct gtaaaaacgt ctggaccggg cgcgaaggca tggagtacgc
     6481 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     6541 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     6601 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga
     6661 ttactacgaa cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca
     6721 tatccctact gctcgcccgc gttcactgat tgacggcaaa cggatggaca aagtgatctg
     6781 gggcccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa
     6841 cttcgaggcc atgcaaaagg agatgtacgg gcagtttgaa aacaccttca tgatgtacct
     6901 gccgcgcctg tgcgaacact gcctcaatcc cagttgcgtg gcgacctgcc caagcggcgc
     6961 tatctacaaa cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg
     7021 gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa
     7081 gtcagaaaaa tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc
     7141 agaaacctgc gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat
     7201 tgaggaagcg gcgagcaccg agcgcgaagt tgacctctat gaacgtcagt gcgaagtatt
     7261 cctcgatcca cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa
     7321 cgtgattgaa gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc
     7381 gctaccgctg caccctgaat atcgcaccct gccaatggtc tggtacgttc ctccgctgtc
     7441 gccgattcag tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc
     7501 catcgaaagc ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac
     7561 cggtccggta ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca
     7621 aaccgtggaa ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca
     7681 ggtcgaagag atgtatcgtt acctcgccat tgccaactat gaagatcgtt ttgtcatccc
     7741 gacgagccat cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac
     7801 ctttggcgac ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat
     7861 cgatgccatc aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc
     7921 aaagtgatcg gcctgttgat ggagtatccg gacgagctgt tgtgggaatg caaggaggac
     7981 gcgctggcgt tgatccgcag cgacgcgccg atgcttacgg atttcaccca caacctgctt
     8041 aacgcgccgc tgctggataa acaggccgaa tggtgcgaag tgtttgaccg tggacgtacc
     8101 acatcgctgc tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg
     8161 gtggacctgc tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc
     8221 gattatctac cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa
     8281 ggattgctca atgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag
     8341 gcaccgtggt acgcgttgtt tgatgctctg ctgcaactgg cgggaagctc cctttcaagt
     8401 gacagtgtca caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat
     8461 gcagtgtggg aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc
     8521 tcgccgctta atcaatatca gcgacgcttt agccaggatg tcgcgccgca atatgtcgac
     8581 atcagtgcgg gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc
     8641 cgtacatttg tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt
     8701 acacctggcg cgcctcctca agtcaaatgc tcgataaacg cgggatggtg atatggtcga
     8761 atctgttcca tatcggcatt ttggggattt tcttcgggca cttgttcggc atgttaacgc
     8821 cgcactggat gtacgcgtgg tttttgccag tggcagcgaa acaactaatg gcgatggtgc
     8881 tcggcggtat ttgcggtgtg ttgacattga ttggcggcgc aggactgctg tggcgcaggc
     8941 tgacaaatca gcgcgtgcgt gcaacttcca ctacgccgga tattatcatc atgagcattt
     9001 tgctgatcca gtgtctgctg gggctaagta ccataccgtt ttcagcacag tatcctgatg
     9061 gtagcgaaat gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat
     9121 cgtcagaaat gcttaacggc gtagcgttcg ttttccgcct gcatctggta ttgggaatga
     9181 ccatcttcct gctcttcccg ttcaccagat tggtgcatgt gtggagcgcg ccgtttgagt
     9241 actttactcg tcggtatcag attgtgcgtt cgcggcgata atttcaccct ggccggatag
     9301 cccttcgctc tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa
     9361 tgaaaccgca ggtgtaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg
     9421 ccggtgttgt tttccccgcc gataatctta gcgaagcgca aatgcagctt atcgcccgcg
     9481 agttaggcca ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc
     9541 gctactttac gccaacggtt gaagtgccga tttgcggtca cgcgacagta gctgcgcact
     9601 atgtacgtgc gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca tcgctggcag
     9661 gaaaacatcg cgtgactatc gaaaagcaca acgatgatta tcgtatttcg ctggaacaag
     9721 gtacgccggg ctttgagcca ccgctggaag gtgaaacacg tgaggcgatt atcaacgtgc
     9781 ttcatctcac tgaagacgat attttgccgg gcttgccaat tcaggtcgca acaacaggac
     9841 actcaaaagt gatgatcccg ttgaaaccgg aagtggatat cgacgccctt tcgcctgatc
     9901 ttgctgcgct gagcgctatc agcaaacaga ttggctgcaa tggtttcttc ccgttccaga
     9961 tccgcccagg caaaaacgaa accgatggtc gtatgttctc gcctgcgatt ggtattgtgg
    10021 aagatccggt gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg
    10081 tattgcccca cgatggcaac gtgttatgtg ttaaaggcca tcaggggcgc gcattggggc
    10141 gtgacggcat gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca
    10201 tttcaggcac agcggtgatt ttgtttcatg ccgaatgggc aattgaactc tgaaaccgtt
    10261 gcctgatgcg cttcgcctgt caggtctaca accgagctaa tgtatgccgg ataaggcgtt
    10321 tacgccgcat ccggcaacct gacataaatt attttcccgc ctccgggtgc gtatcaaacg
    10381 ccgccatcac cagcgctaac tcatccacgg taaagccatg tttcgcatca tccacgccca
    10441 gaccaaactg ttcttgcatc acagcatata atgacgccac atccggtaga tttcgctgct
    10501 ccaccgcgtg cccattttca taatgggtaa aatgaaaatt ggtcagcgtt agcttgccac
    10561 cgtccggcaa atggcggcac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg
    10621 ccgaccagaa attgcccatc acataatcgc tttgttgctg ctcgcagaga tcaaaacggt
    10681 acatcgactg ccagtgctga tgatgattaa actgcaacac ccaatcatca ccctcctgca
    10741 acaaccgata atctccgtgt ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg
    10801 cggttagcgt ctgcccaccg aaaccgacat cagcaatcca tttttcctct tccagttcca
    10861 ccaacagcaa acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgcc
    10921 ctaacaagct gcgaacgtta aactccagct cgcgtaacac ccgctcaaac acgccattct
    10981 gctcaaaaca gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat
    11041 caagctgtat ttccctcggc agcaaaacgt cgaggttttc aaacggaata gtgcaattgt
    11101 gtttcaggtg caatgcacga agcgtatcaa tattgaccgc agcagctccc gaccagttaa
    11161 tacgggcaaa atagtgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc
    11221 aacttataac ctggaataga cgccgcaccg gctattttgt gcgatcgcgg acatgcatca
    11281 cgtttgcaaa tcgccaatcc cgttaatttc gttacttatt gcgttacgga ctacgcttaa
    11341 aaaaaccttc ttcgcgaggc gataccatga gccgattcat ccccatagaa ttacaccatg
    11401 ccagccgtct gttaaatcat ggcccaactg tcatgatcac cagtttcgat gagcaatccc
    11461 agcggcgtaa cattatggca gcagcctggt caatgccggt ggagtttgaa ccgccacgcg
    11521 tggcgattgt ggtagataaa tcgacatgga ccagggagtt gattgaacgt aacggtaaat
    11581 ttggcatcgt tatcccgggc gttgcagcaa ctaactggac gtgggcagtg ggaagcgtgt
    11641 cgggtcgtga agaagataaa tttaattgct atggcattcc ggtggtgaaa ggtccggtac
    11701 ttggtttgcc acttgttgaa gagaaatgcc tggcgtggat ggagtgccgc ctgctacctg
    11761 cgacttctgc gcaacaaaaa tacgacacgc tgtttggcga ggtggtatca gcagcggcag
    11821 acgcacgggt atttgtcgaa ggccgctggc agtttgatga tgataagctc aatacgttgc
    11881 atcatttagg tgctggcacg tttgttacca gcggcaagcg agttacggcg ggttaagcat
    11941 tacatgctgt aaccgggttt ctttatcaaa gcctccattt ggggcgcgat ttcggcatcc
    12001 cagatagctt gccaagattc tggttgaatc tgctgtagag caatgcttat cgagctatct
    12061 ttactgttca gatggcgaat aataacgtcg gtaatatctg cagcaagtgc tgctttttgt
    12121 tgttcgtcca gttcacgcgg aaaacattta atgtcgatgt gcggcatgat tgtgctcctt
    12181 ttgaaagatg tcagtaaaat taacacacat tgtgaatgtc gtgtgtccgg taatgcgttt
    12241 ttacgacaat aatgccaggg caagattacg aaagccttct ccccgcaagg actgacgcca
    12301 ggtagtttct gtccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    12361 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    12421 ttccttttct tattttgcag tcgtcttcat tcattaccac gtccagacgc cagtgcagct
    12481 tattctccac gtgccagtgg tttcggattg ctgtggcgaa cttctctgcg gttaaatcag
    12541 cagaactgat ataatatctg accgtcattt ctggctcttt cttttgttct gctattatgg
    12601 accgaaagga gactgccacg cataatttct tcagcccttt ccattcaaac gtgaaatcaa
    12661 taagttcatc agggacatcg caaacaatat gaagacggat ttcttctctg ccgtgactct
    12721 tttcactcat tgcgtaactg tcat
//
LOCUS       DAZGTR010000124        12711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGTR010000124 DAZGTR010000000
VERSION     DAZGTR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.126"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..105
                     /locus_tag="ACHLCY_003901"
     CDS             <1..105
                     /locus_tag="ACHLCY_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0371070.1"
                     /translation="NHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            102..308
                     /locus_tag="ACHLCY_003902"
     CDS             102..308
                     /locus_tag="ACHLCY_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP0371071.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            286..957
                     /locus_tag="ACHLCY_003903"
     CDS             286..957
                     /locus_tag="ACHLCY_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFP0371072.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKAEELPLIIELVSKDKKYVICHADYPFDEYEFGKPVDHQQVIW
                     NRKRISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGAWA"
     gene            948..1466
                     /locus_tag="ACHLCY_003904"
     CDS             948..1466
                     /locus_tag="ACHLCY_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0371073.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKRAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRSGEGWRKPSMATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKSLDMQLSI"
     gene            1530..1604
                     /locus_tag="ACHLCY_003905"
     tRNA            1530..1604
                     /locus_tag="ACHLCY_003905"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1563..1565,aa:Asn,seq:gtt)
     gene            1610..1685
                     /locus_tag="ACHLCY_003906"
     tRNA            1610..1685
                     /locus_tag="ACHLCY_003906"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1643..1645,aa:Thr,seq:tgt)
     gene            1928..2251
                     /locus_tag="ACHLCY_003907"
     CDS             1928..2251
                     /locus_tag="ACHLCY_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFP0371074.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2235..2711
                     /locus_tag="ACHLCY_003908"
     CDS             2235..2711
                     /locus_tag="ACHLCY_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP0371075.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDVYRNQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADNLIAKFKEAGGTVREIEV"
     gene            2708..3145
                     /locus_tag="ACHLCY_003909"
     CDS             2708..3145
                     /locus_tag="ACHLCY_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0371076.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            3346..3870
                     /locus_tag="ACHLCY_003910"
     CDS             3346..3870
                     /locus_tag="ACHLCY_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0371077.1"
                     /translation="MNNHSVIPAFDFREMVQAKNGEVVTTSRKIAKYFGKRHGDVLRK
                     IEQVKADCSREFSQRNFASADYIDEQGKVRPMYSLTKDGWIMVVMGFTGKAAAAIKES
                     YIAAFNWMAEQLSRRMAIGEEMQHRYAIKETRSKLKGTIGSRLMNERKKEKRVLAVEH
                     EYILQVTQPELLIN"
     gene            4102..4482
                     /locus_tag="ACHLCY_003911"
     CDS             4102..4482
                     /locus_tag="ACHLCY_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFP0371078.1"
                     /translation="MNDQQIEKEIVEKGKTEPRITPQHIEDVIKSEHYFTAYDGRNGA
                     ISSNEYCGREKPEEGDRDLSPLKLLTFCVLVLKNGFTVTGESACASQGNFDAEIGRKI
                     ARQNAVNKIWMLEGYLLKQKLSEQ"
     gene            4586..4828
                     /locus_tag="ACHLCY_003912"
     CDS             4586..4828
                     /locus_tag="ACHLCY_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HFP0371079.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITTRTIEAVRKSKEALDLFTTGV"
     gene            4832..5119
                     /locus_tag="ACHLCY_003913"
     CDS             4832..5119
                     /locus_tag="ACHLCY_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371080.1"
                     /translation="MTEQEMPRYQCHKKVRALKIGSIEHKPNPDQPGKSGSSSYGAII
                     HPDDKKYAAFDVSAEYICKHRPMSGGYYVVYEDGYESYSPAEVFESGYSKL"
     gene            5129..5308
                     /locus_tag="ACHLCY_003914"
     CDS             5129..5308
                     /locus_tag="ACHLCY_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371081.1"
                     /translation="MTSVVDLGKEKKFPITQELYERLESVIHDYDGEISLCEAIGTLE
                     LLKQSLIEGAKESSA"
     gene            5332..5754
                     /locus_tag="ACHLCY_003915"
     CDS             5332..5754
                     /locus_tag="ACHLCY_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin carboxyl-hydrolase"
                     /protein_id="HFP0371082.1"
                     /translation="MATEPKAGRPSDYMPEVADDICSLLSSGESLLKVCKRPGMPDKS
                     TVFRWLAKHEDFRDKYAKATEARADSIFEEIFEIADNAIPDAAEVAKARLRVDTRKWA
                     LARMNPRKYGDKVTNELVGKDGGAIQIETSPMSTLFGK"
     gene            5751..7166
                     /locus_tag="ACHLCY_003916"
     CDS             5751..7166
                     /locus_tag="ACHLCY_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFP0371083.1"
                     /translation="MTSINPIFEPFIEAHRYKVAKGGRGSGKSWAIARLLVEAARRQP
                     VRILCARELQNSISDSVIRLLEDTIEREGYSAEFEIQRSMIRHLGTNAEFMFYGIKNN
                     PTKIKSLEGIDICWVEEAEAVTKESWDILIPTIRKPFSEIWVSFNPKNILDDTYQRFV
                     VNPPDDICLLTVNYTDNPHFPEVLRLEMEECKRRNPTLYRHIWLGEPVSASDMAIIKR
                     EWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINE
                     GADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAP
                     YQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLS
                     FDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCM
                     HCPESAAQPDYSSYSIPCGVG"
     gene            7168..9366
                     /locus_tag="ACHLCY_003917"
     CDS             7168..9366
                     /locus_tag="ACHLCY_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0371084.1"
                     /translation="MAEKKMTDWHRKVLCNFDNAWSATQDMREQIIEAQRFVRVSGAQ
                     WEGSTNAGYSFDEGRFEHYPRFELNKIARECDRIIGEYRQNRISVKFRPKDDKASEAL
                     AEKMNGKFRADYQETSGGEACDNAFDDAVTGGFGCFRMCADYEDEMDPSNEQRRISLL
                     PVYDPATCVFFDQDSKQYDRSDAMWAMEMFSMTPKAFEAEYPDSIAASLSRDDTGTQY
                     DWSTPDAIYVGRYYEVRIEKVKLTAWRNPVSGETAIYDEDQIKDIVDELTDGAFELIG
                     ERTVKKRRVYCGLLSGAEWLEEPKRIPGEHIPLIPVYGRRSFVDNQERIEGHAAKAMD
                     AQRLENLMVSMIADNATQAGGDGIPVVDVDMIPGPLATHWAERNKKRPAFLPMVSLKN
                     KNGDITAQAQVSSYTPPTQMPPALAGLLQYTGTAIQQITGASQLENMPSNVATDTVDS
                     IFNRMDTQSYIYMDNMAKSMRRAGVVWLSMAREVYGSDTPMRIVNEDGSDDVALMTDE
                     VVDRQTGQVIALNDLSQGNYEVTVDVGQSFATRRDATVKSLLSMLALIPPGTPKHDLV
                     SSMILDNMDGEGMDDLKEYNRNQLLLSGVIKPRTPEEQQMVEQAKQQQASQPDPAMVA
                     AQGQLLAGQAELQKAQNEQASIQVKAFQAQTDAQVAAANVVKILASADSQQKSDIREA
                     LKLLGQFQQQQGDNARADAELVLKSQAQGHAQRMDISSILQKPTQQQPQQ"
     gene            9457..10350
                     /locus_tag="ACHLCY_003918"
     CDS             9457..10350
                     /locus_tag="ACHLCY_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HFP0371085.1"
                     /translation="MDQMAENTPEVEIETDASEQIPDDVELAEEVETEDGSESSGNDA
                     EEATETDDDESEQEFYFGDEKLDSPTSEDGAEHGLVKHLRKTIKEKDRELKELMRQSQ
                     KPVEQQPVITQPPRMPKLDDEDIGFDEEIYQQRMAKWAEDNGKYQQQEMARKQKEQEL
                     QAAYQERLSKYQQRVKALKVPGYQEAEQAVLEEIPIETQNAILFESEKPEIVVLALGR
                     NAELRKQLAEATNPVAIGRLLERIESKARIMPKAKTTAATTPTVKGSNGAVINNLGKL
                     KAKALETGDWTPYFAAKKAKK"
     gene            10369..11622
                     /locus_tag="ACHLCY_003919"
     CDS             10369..11622
                     /locus_tag="ACHLCY_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HFP0371086.1"
                     /translation="MANQLAKDLEIMFENYVEGFEAACVVSRNAKKFRSGDTAMQRAG
                     DVLYRPQHYHMNIEEGLDLSGKTPTALVQRLVPSVFKEPKNILYTLDAREMRDPEHKT
                     EAGRAAGMRLAAQIDSDLISMVTQRATNVITMADSTTGSQGRDLWNCAAGIDATMTAI
                     GVPQGINRRSFWNPFNYKDLAGELGHRAYAQGATLTAYEKAQIPPVASFDSYKTDISG
                     RLPKGSAESLTVSGQPEHKVEAKDSNGMPVDNRQGTITVSASGLQVGDAFTIAGVNSV
                     HQITKDTTGQPQVFRVLAVSGTTVTISPKILPVENTDVASRPYANVDAKPAASAAITI
                     LNKNAAPANLFWADGSVELMYGKLAFPTGQGPQVMTATTEQGATLIMSYAFDHIKGVT
                     TARFTTLYGCSVLVPEYTGIVIAGQ"
     gene            11664..11852
                     /locus_tag="ACHLCY_003920"
     CDS             11664..11852
                     /locus_tag="ACHLCY_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371087.1"
                     /translation="MARTMLYKSGNMITCGQFAVDYIIVDDEEVKSHLKKGWVKTPEE
                     TATKQKVAKAEEDGENEG"
     gene            11833..12294
                     /locus_tag="ACHLCY_003921"
     CDS             11833..12294
                     /locus_tag="ACHLCY_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization gp4 family protein"
                     /protein_id="HFP0371088.1"
                     /translation="MAKTKGDLVLKALRKAGLYSNATLTDADPQAIEDAINDLEDMMA
                     AWQAKGIELGYQFADTENGIMPLPDDDSGIPAWANDGVALKLAVQVCMDNVIQPSDAL
                     LTAADSAYQTICIALTKIPPLERRNDMPRGSGNKSAFTWNRFYIEKDDPST"
     gene            12304..>12711
                     /locus_tag="ACHLCY_003922"
     CDS             12304..>12711
                     /locus_tag="ACHLCY_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019073 - viral DNA genome packaging
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein"
                     /protein_id="HFP0371089.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQSAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKRVSNWPTDSGFTQY"
BASE COUNT         3591 a         2928 c         3316 g         2876 t
ORIGIN      
        1 aaccataacc gccatcgctg gactgtcgaa gaatgcaggg cgatcaaggc gaagtatcag
       61 cagaaactta aagacctgcg aaacagcaga agtgaggccg catgacgttc tcagtaaaaa
      121 ccattccaga catgctcgtt gaagcatacg gaaaccagac agaagtagca cgcagactga
      181 aatgtagtcg cggtacggtc agaaaatacg ttgatgataa agacgggaaa atgcacgcca
      241 tcgtcaacga cgttctcatg gttcatcgcg gatggagtga aagagatgcg ctattacgaa
      301 aaaattgatg gcagcaaata ccgaaatatt tgggtagttg gcgatctgca cggatgctac
      361 acgaacctga tgaaaaaact ggagacgata ggattcgaca ccaaaaaaga cctgcttatc
      421 tcggtgggcg atttggttga tcgcggtaca gagaacgtcg aatgtctgga attaatcaca
      481 ttcccctggt tcagagctgt acgtggaaac catgagcaaa tgatgattga tggcttatca
      541 gagcgtggaa acgtcaatca ctggctgctt aatggcggtg gctggttctt taatctcgat
      601 tacgacaaag aaattctggc taaagctctt gcccataaag cagaagaact tccgttaatc
      661 atcgaactgg tgagcaaaga taaaaaatat gttatctgcc acgccgatta tccctttgac
      721 gaatacgagt ttggaaagcc agttgatcat cagcaggtaa tctggaaccg caaacgaatc
      781 agcaactcac aagacgggat cgtgaaagaa attaaaggcg cggacacgtt catctttggt
      841 catacgccag cagtgaaacc actcaaattt gccaaccaga tgtatatcga taccggcgca
      901 gtgttctgcg gaaatctcac attgattcag gtacagggag aaggcgcgtg ggcataagag
      961 aactaaacct caccaaagaa cagcatgagt ggctgaatgg ctggcttgaa ctgtggggcg
     1021 catgggttta ttcaggtcgt ctggaaaagc gtatgagcag cgtaatagcg aagttcatgg
     1081 agagcgtaga gccgggaaga gttatgacaa ggccaatgtg taatgatgat gatggaatgt
     1141 tgatttctca ggtcgtcgat tccgtcatgt acattgacaa gagagccttt ggcatcctcc
     1201 tcagctacta cgctcatggt tcatctaagc gagcaattgc atcctactat cacgcgactg
     1261 caaagccacg caagatgtgt gggcgtagtg gcgagggatg gagaaaacct tcaatggcaa
     1321 cctgtagaaa cgaaattgac gacatcctga aagcgtcgtt atttgttttg taccagccaa
     1381 tgcaaaatgc tttcaaaatg cgtaaacgtg ttgagaaagt taagcatgtt gctgttaaaa
     1441 gccttgacat gcaattatcc atttagccat aattagaagg taagctgccg ttagtgactc
     1501 ttaagttgca acggtggatt tttttatttg ggtcagtcgt ataaaggtca ttacggaagg
     1561 ctgttaacct tcttatcgtg gttcgagtcc acgctgtccc gccaaatatg ctggtttagc
     1621 tccaatggta gagcagtcgc cttgtaagcg aatgggtagc ggttcaagtc cgttaaccag
     1681 caccataact gagccgtagc cactggctat cctgaactca tcagtgatag ttacgctgcg
     1741 gccttctaca catgatcttc gtgaaagcgg gtggcaggag gctgcgctaa caacctcctg
     1801 ccgttttgcc cgtgcatatc ggtcacgaac aaatctgatt actaaacaca gtagcctgga
     1861 tttgttctat cagtaatcga ccttattcct aattaaatag agcaaatccc cttattgggg
     1921 gtaagacatg aagatgccag aaaaacatga cctgttagcc gccattctcg cggcaaagga
     1981 acaaggcatc ggggcaatcc ttgcgtttgc aatggcgtac cttcgcggca gatataatgg
     2041 cggtgcgttt acaaaaacag taatcgacgc aacgatgtgc gccattatcg cctggttcat
     2101 tcgtgacctt ctcgacttcg ccggactaag tagcaatctc gcttatataa cgagcgtgtt
     2161 catcggctac atcggtactg actcgattgg ttcgcttatc aaacgcttcg ctgctaaaaa
     2221 agccggagta gaagatggtg gaaatcaata atcaacgtaa ggcgttcctc gatatgctgg
     2281 cgtggtcgga gggaactgat aacggacgtc agaaaaccag aaatcatggt tatgacgtca
     2341 ttgtaggcgg agagctattt accgattact ccgatcaccc tcgcaaactg gtcacgctaa
     2401 acccaaaact caaatcaaca gccgctggac gttaccagct tctttcccgt tggtgggatg
     2461 tctaccgtaa tcagcttggc ctgaaagact tctctccaaa aagccaggac gctgttgcgc
     2521 tgcagcagat taaggagcgt ggcgctttac cgatgattga tcgcggtgat attcgtcagg
     2581 caattgaccg ttgcagcaat atctgggctt cactgccggg cgctggttat ggtcagttcg
     2641 agcataaggc tgacaacctg attgcaaaat tcaaagaagc aggcggaacg gtcagagaga
     2701 ttgaggtatg agcagagtaa ccgcgattat ctccgctctg gttatctgca tcatcgtctg
     2761 cctgtcatgg gctgttaatc attaccgtga taacgccatg acctacaaag agcagcgcga
     2821 taaagccaca tccatcatcg ctgatatgca gaagcgtcaa cgtgatgtag cagaactcga
     2881 cgccagatac acaaaggagc ttgctgatgc taacgcgact atcgaaagtc tccgtgctga
     2941 tgtttctgct ggtcgtaagc gcctgcaagt cgccgccacc tgtgcaaagt caacgaccgg
     3001 agccagcagc atgggcgatg gagaaagccc aagacttaca gcagatgctg aactcaatta
     3061 ttaccgtctc cgaagtggaa tcgacaggat aaccgcgcag gttaactacc tgcaggagta
     3121 catcaggact cagtgcctga aataattttt ttgcaaacca caaagtccat ttaatgagcc
     3181 tcgcgaaaag cggggctttt ttatgtccgc agtaaacgcg catctcacgc gcatattaac
     3241 gagagccttt cagtaagcga gcctgagaaa tgccgttata ggtggcgacc tctctcgggc
     3301 ggcttttctg tgagacaggc tcactttcta aaaggtaaag acgctatgaa taatcattca
     3361 gttattccag ccttcgactt ccgagaaatg gtgcaagcca aaaacggaga ggtcgttacc
     3421 acatccagaa aaattgccaa gtacttcggc aagcgacacg gtgatgttct caggaaaatc
     3481 gagcaggtta aggctgattg ctcacgtgag tttagccaac gcaattttgc gtcggctgat
     3541 tatatcgatg agcagggcaa ggttcgcccg atgtacagcc tgacgaaaga tggctggatc
     3601 atggttgtga tggggttcac cgggaaagct gctgcggcaa tcaaggagag ctatatcgca
     3661 gcattcaact ggatggcaga gcaactgagc cgccgcatgg caattggcga agaaatgcag
     3721 caccgctacg ccatcaaaga aacacgctca aagctgaaag gtacgatcgg cagtcggtta
     3781 atgaacgaac ggaagaaaga gaagcgcgtc ctggctgtcg agcatgaata catcttgcag
     3841 gtgacacagc ctgaactgct gattaattga agatgtcatt acaaagccta tctacgggtg
     3901 ggcttgataa tgaaaccgga gttaatttct ggtcactaat taacggcagt acagcgaaac
     3961 aacccaagcc agtaagtggg gaaataacac tggcagccac tgaaagatga acctcctgcc
     4021 ttatggcaaa aaagattctt tgtggtggcg gactgatgga aagacatcct aatcaagcaa
     4081 ccactccaca gggtcataat tatgaacgac cagcaaatcg aaaaagaaat cgttgagaaa
     4141 ggcaaaacgg aaccgcgaat caccccgcag cacatcgaag acgtgattaa aagcgagcat
     4201 tactttactg cttatgatgg acgaaatggt gccatttcca gcaacgaata ttgtggcagg
     4261 gaaaaaccag aggaaggcga tcgtgattta tcaccattga agttgctcac tttctgcgta
     4321 ctggtgctga agaatggctt caccgtcacc ggagagagtg cctgtgcaag ccagggaaac
     4381 tttgatgcag aaattggtcg gaagattgct cggcagaatg ctgtaaacaa aatctggatg
     4441 ctcgaaggtt acttgctgaa gcagaagcta agcgaacagt agttattaca aaagccattc
     4501 cctacagagt ggctttgaca atggcttata ccctacacgg gataacttaa ctgatatccc
     4561 ttttaacgga taaacggagc caacaatggc agagattatt cccatgactg aagaacagaa
     4621 attccagtta gagatttaca aactggtcat gaaccagaac gcagccgcag aagaagcatt
     4681 tcaattcatt ggcactgacg aactgaagct tgagctattc aaaattcact tccagtcagg
     4741 tggcgctaat tcagatatca cgacccgcac tatcgaagcg gtgcgtaaat cgaaggaagc
     4801 gttagacctg ttcactaccg gagtgtaaga gatgactgaa caagaaatgc cgagatacca
     4861 gtgccacaaa aaagttcgcg ccctgaagat tggctctata gaacataagc caaacccaga
     4921 tcagcctggt aagtctggct cttctagtta tggggcaatt attcatccgg atgataagaa
     4981 atacgcagca tttgatgtta gcgcggaata tatctgtaag caccgaccaa tgtctggagg
     5041 ctattacgtt gtctatgagg atggatatga atcatattct cctgctgagg tatttgagtc
     5101 tggatattca aaattatagg aatcctctat gacaagcgtc gttgatcttg gtaaggagaa
     5161 gaaattccca attactcaag agctatacga gaggctggaa agcgtcatcc atgattacga
     5221 tggtgaaatc agtttatgcg aggcgattgg cacactcgaa ttgctgaagc agtcactgat
     5281 tgaaggcgcg aaagagtcct cagcctgaaa taacaactaa gtgagatgaa tatggcgact
     5341 gaaccaaaag ctggtcgccc ctctgattat atgccggagg tggctgacga tatctgctcg
     5401 ttgctttctt ctggcgaaag tttgctgaaa gtatgtaagc gtcctggtat gccggataag
     5461 tccactgttt tccgctggtt ggcaaagcat gaggattttc gcgacaagta cgcgaaggca
     5521 actgaggcac gagctgattc tattttcgaa gagatattcg aaattgctga caatgcgatt
     5581 ccagatgctg ctgaggtggc aaaggcaaga cttcgcgttg atacccgcaa atgggcgctg
     5641 gcccgaatga atccccgtaa gtatggcgac aaggtaacta acgagcttgt cggtaaggac
     5701 ggcggcgcaa ttcagattga aacatcaccg atgagcactc tattcggaaa atgacctcga
     5761 ttaatcctat ctttgaaccg ttcattgagg cgcatcgcta caaagtcgcc aaaggcggtc
     5821 gaggtagcgg taagtcatgg gcaatcgcga ggctgcttgt tgaagcggcg cgtcggcagc
     5881 cggtgcgcat cctctgtgct cgtgaactgc aaaacagtat cagtgattcg gtaatccggt
     5941 tgcttgaaga caccatagag cgggaagggt attcggctga gtttgaaatt cagcgttcaa
     6001 tgattcgtca tctcggaacg aatgctgaat tcatgttcta cggcataaaa aacaacccga
     6061 cgaagattaa atcgctcgaa ggtattgata tctgctgggt ggaagaagcg gaagcggtaa
     6121 cgaaggaatc atgggatatc ctgataccga ccatccgtaa gccgttctct gaaatatggg
     6181 tgagctttaa cccgaaaaac atcctcgacg atacctatca gcgattcgtt gtaaatcctc
     6241 ccgatgatat ttgcctgctg acggtgaact acaccgacaa cccgcacttt cctgaagttc
     6301 tccgtctgga gatggaagag tgcaaacgca gaaatccgac actgtatcgt cacatctggc
     6361 ttggtgagcc agtaagcgca agtgatatgg caatcatcaa acgtgaatgg cttgaagccg
     6421 caaccgatgc gcacaagaaa ctcggatgga aggcgaaagg cgctgttgtc tctgcgcatg
     6481 acccgtcaga tacaggaccg gatgctaaag gttatgcatc gcgtcacggt tcggtggtta
     6541 agcgcattgc cgaaggtctg ctgatggaca tcaacgaggg tgctgactgg gctacttcgc
     6601 tggcgattga agacggcgct gatcattacc tgtgggatgg cgatggtgtc ggtgcagggc
     6661 tacgcagaca gacaacggaa gcgttctccg gcaagaaaat caccgccacg atgttcaagg
     6721 gcagcgaatc gccattcgat gaagacgcgc cgtatcaggc cggagcatgg gctgatgaag
     6781 tcgtacaggg tgacaacgtt cgcactattg gcgatgtttt ccgcaataag cgagcgcaat
     6841 tctattacgc actggctgat aggctgtatc tgacatatcg ggcggttgtt cacggtgagt
     6901 atgcagaccc cgacgacatg ctgagtttcg acaaagaagc gataggcgag aagatgctgg
     6961 agaagctgtt tgcagaactg acgcagattc agcgcaaatt caataacaac gggaagcttg
     7021 agctaatgac taaggtcgaa atgaagcaga agctcggtat tccatctcct aacctggctg
     7081 atgcgctgat gatgtgtatg cattgcccgg agtcggctgc gcaacccgac tattccagtt
     7141 actcaattcc ttgtggtgta ggttgatatg gcagaaaaaa agatgactga ctggcatcgc
     7201 aaggtgctgt gcaactttga taatgcctgg tcagcaacgc aggatatgcg tgagcagatt
     7261 attgaggctc aacgtttcgt ccgggtatcc ggcgcacagt gggaaggcag cacaaacgct
     7321 ggttactcat ttgatgaagg caggtttgag cattacccgc gctttgaact gaataagatt
     7381 gcccgtgaat gtgatcgcat cattggcgag tatcgacaga atcgcatcag cgttaaattc
     7441 aggccgaagg acgataaggc atcggaagcg ttagccgaaa agatgaatgg taaattccgc
     7501 gctgactatc aggaaacatc cggtggtgaa gcgtgtgata acgcatttga tgatgctgta
     7561 acgggcggat tcggttgttt ccgcatgtgt gccgattacg aagatgaaat ggatccgagt
     7621 aacgagcaac gccgtataag ccttctccct gtttacgacc cagcgacatg cgtcttcttc
     7681 gatcaggaca gcaagcaata tgaccgctct gatgctatgt gggctatgga aatgttctcc
     7741 atgacgccta aagcgttcga agctgaatac cctgattcca tcgcggcaag cctttctcgt
     7801 gatgacactg gtactcagta tgactggtca acgcccgatg ccatctatgt tggacgctac
     7861 tacgaagttc gcatagagaa ggtgaagctc acagcatggc gtaaccctgt tagcggagaa
     7921 acggcaatct atgatgaaga tcaaatcaaa gatattgtcg acgagctgac cgatggcgca
     7981 ttcgaactga ttggtgagcg gacagtgaag aaacgccgag tttattgcgg tcttctgtct
     8041 ggcgctgaat ggctggaaga accgaagcgt attccgggcg aacatattcc tctcatcccg
     8101 gtatatgggc gtcgctcatt tgttgataat caggagcgaa tcgaaggtca cgcagcaaaa
     8161 gcgatggatg cacagcgtct tgagaacctg atggtttcca tgattgcaga taacgctact
     8221 caggctggcg gtgatggcat tcctgtagtt gatgttgaca tgattcctgg tcctctcgcc
     8281 actcattggg cggagcgcaa caaaaagcgc ccggcgttcc tgccgatggt cagtctgaaa
     8341 aacaaaaacg gagatattac tgcgcaggct caggtcagca gttatacacc tccgacacaa
     8401 atgcctcctg ctcttgccgg gctattgcag tacaccggaa cggctattca gcaaattaca
     8461 ggtgcgtcgc agcttgagaa catgccgagc aacgtcgcca ccgataccgt tgatagcatc
     8521 tttaaccgga tggacacgca gtcctatatc tacatggaca atatggctaa atccatgcgc
     8581 cgcgctggcg tcgtgtggct ttctatggct cgtgaagtct atggcagtga tacgccgatg
     8641 cgtatcgtta atgaggacgg cagcgatgac gtggcgctga tgactgatga agtggttgac
     8701 cgtcagacag ggcaggttat cgcgcttaac gacctttcgc agggtaacta tgaagtgact
     8761 gtcgatgtcg gtcagtcgtt cgctactcgc cgtgacgcaa cggttaagtc gttactttcc
     8821 atgctggcac ttatcccacc aggaacgccg aagcacgacc ttgtatcgtc gatgattctc
     8881 gacaatatgg acggcgaagg gatggacgac cttaaagaat acaaccgcaa tcagttgctt
     8941 ctgtctggag ttatcaagcc gagaacgcca gaagagcagc aaatggttga gcaggcgaaa
     9001 caacaacagg ccagtcagcc agatccggct atggttgctg cgcaaggtca gcttcttgct
     9061 ggtcaggctg aattgcagaa agcgcagaac gaacaggcat ccattcaggt taaagcattc
     9121 caggcacaga ctgatgctca ggttgcagcg gcaaatgttg tgaaaatcct cgcatctgcc
     9181 gatagccagc agaaatctga tatccgcgag gctctgaaac tgctaggaca gttccagcaa
     9241 cagcaaggag acaatgcccg tgctgatgca gagcttgtcc tgaaaagtca ggcacagggc
     9301 catgcgcagc gcatggacat cagcagtatc ctgcaaaaac caactcagca acaaccacag
     9361 cagtaattaa cccataacgt gcaatggctg tctttatgag gcctggcacc ctattgcctt
     9421 ccgatgggct gaacatcgag taaacagggg taacaaatgg accagatggc agaaaacaca
     9481 ccagaagttg aaatcgaaac cgacgcgtca gagcagattc ctgatgatgt cgaactggct
     9541 gaagaagtcg aaacagaaga tggcagtgag tcctccggca atgatgcaga ggaagctact
     9601 gaaactgatg acgacgaatc agaacaggaa ttctactttg gtgacgaaaa gctggattcg
     9661 ccaaccagcg aagatggcgc agagcatgga ctggtaaaac acctgcgcaa gacgattaaa
     9721 gagaaagacc gcgagctgaa agagctgatg cgtcagtctc agaaacccgt cgagcagcag
     9781 ccggtaatca ctcaaccacc gcgaatgcca aaactggatg atgaggacat cggtttcgat
     9841 gaagaaatct accagcaacg catggctaag tgggcagagg ataacggcaa gtaccagcaa
     9901 caggagatgg ctcgcaagca gaaggagcag gagcttcagg ctgcctatca agagcgatta
     9961 tccaaatatc agcaacgtgt taaggctctc aaagttcctg gctatcagga agcagaacag
    10021 gccgtactcg aggaaatccc catcgagaca caaaacgcga tcctgtttga gtcagagaag
    10081 ccggaaatcg ttgttctggc actcggtcgc aacgctgaac tgcgcaagca actggcagaa
    10141 gctaccaacc ccgtagcaat tggtcgtctg ctggaacgta tcgaatcgaa ggccagaatc
    10201 atgccaaaag caaaaaccac ggcagccaca accccgacag ttaaggggag caacggcgca
    10261 gtaatcaaca acctcggcaa attgaaagcc aaggcgctgg aaactggtga ctggacgccg
    10321 tatttcgccg ctaaaaaggc aaaaaaataa cctatcggag cattaagcat ggctaaccaa
    10381 ttagcaaaag accttgaaat catgttcgaa aactacgttg aaggctttga ggccgcctgc
    10441 gtagtttccc gtaacgctaa aaaattccgt tccggtgata cagcaatgca gcgagcaggt
    10501 gatgttctgt atcgtccgca gcattaccac atgaatattg aggaaggcct agacctcagc
    10561 ggcaaaacgc caacagcact ggttcagcgc cttgttcctt ctgtgttcaa ggagccgaaa
    10621 aacattctgt acactctgga tgcgcgtgaa atgcgtgacc cggaacataa aactgaagct
    10681 ggtcgagccg caggtatgcg ccttgctgca cagattgact ctgacctgat ttccatggtc
    10741 acgcagcgtg ctactaacgt gatcacgatg gctgactcaa ccacaggttc acagggccgt
    10801 gatttgtgga actgtgcggc aggtattgat gccaccatga cggcgattgg tgtaccgcag
    10861 ggtatcaacc gtcgctcttt ctggaacccc ttcaactaca aagaccttgc tggcgagctt
    10921 ggtcaccgtg cctacgctca gggcgcaacc ctgacagcat acgaaaaagc gcagatccct
    10981 ccggttgctt cctttgatag ctacaagacc gatatttctg gtcgattacc gaaaggaagc
    11041 gctgaatcct tgacagtatc aggccaacct gaacacaagg ttgaagcgaa agattcaaat
    11101 ggtatgccag ttgataaccg acaggggact attacggtat ctgcatctgg cttgcaggtt
    11161 ggtgatgcgt tcaccattgc cggtgtgaat tccgtacacc agatcacaaa agataccacc
    11221 gggcaaccgc aggtattccg tgttctggct gttagcggaa ctaccgtaac aatctctcca
    11281 aagattctcc ctgttgaaaa taccgatgtt gcgagtcgtc catatgcaaa cgtcgatgcc
    11341 aaaccggcag catcagcagc aatcaccatt ctcaacaaga acgcagcacc tgctaacctg
    11401 ttctgggctg atggttctgt tgagctgatg tacggcaaac tggcgttccc gactggtcag
    11461 ggtccacagg taatgacagc aaccaccgag cagggcgcta cgctgatcat gtcttacgcc
    11521 ttcgaccaca tcaaaggcgt aaccactgct cgtttcacca ctctgtacgg ttgctctgta
    11581 cttgttcctg aatatacggg catcgttatt gccgggcagt aattttggtg gggcttcggc
    11641 cccattttta ttgggagaag acaatggcac gaacaatgct ctataagtcg ggcaacatga
    11701 tcacctgtgg tcagtttgct gtcgattaca tcattgttga tgacgaagaa gttaaatctc
    11761 acctgaaaaa aggctgggta aaaacccctg aagaaaccgc aacgaagcaa aaagtggcta
    11821 aggcggaaga agatggcgaa aacgaagggt gatctcgttc ttaaggcttt acgaaaagct
    11881 gggctgtatt ccaatgccac gttgacagat gctgaccctc aggcaattga agatgccatt
    11941 aatgacctcg aagacatgat ggcagcatgg caggctaaag gtatcgagct tgggtatcag
    12001 tttgctgata cagaaaacgg catcatgccg ttacctgacg atgattcagg tatccctgca
    12061 tgggcaaatg atggcgtcgc tttgaaactc gctgtgcaag tgtgcatgga taacgtcatt
    12121 cagccgtcag acgctctcct taccgctgct gacagtgcat atcagacaat ctgtatcgct
    12181 ttaaccaaaa taccaccact tgagcggcga aatgacatgc ctcgcggtag tggtaacaaa
    12241 agcgcgttta cgtggaatcg gttttacatc gagaaagatg atccgagtac gtgaggtgaa
    12301 taaatgccga ttcagcaact tccgcttatg aaaggtgtcg gcaaagactt tagaaacgcc
    12361 gactatatcg actatctgcc agtgaatatg ttggctacac ccaaagaaat cctgaacagc
    12421 agcggatatc ttcgctcatt cccgggcatt gccaaacgtt ctgatgtgaa cggcgtatcg
    12481 cgcggagttg agtacaacat ggcgcagagt gctgtttatc gcgtgtgtgg cggcaagctg
    12541 tacaaaggag aaagtgaagt cggtgatgtt gccggaagtg gtcgtgtatc aatggcgcat
    12601 ggtcggacat ctcaggctgt aggcgttaat ggccaactgg tcgagtatcg ttatgatggc
    12661 acggttaaaa gagtctcaaa ctggcctaca gacagcggat tcacgcagta t
//
LOCUS       DAZGTR010000125        12702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGTR010000125 DAZGTR010000000
VERSION     DAZGTR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.127"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            102..1616
                     /gene="caiT"
                     /locus_tag="ACHLCY_003923"
     CDS             102..1616
                     /gene="caiT"
                     /locus_tag="ACHLCY_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP0371090.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1647..2789
                     /gene="caiA"
                     /locus_tag="ACHLCY_003924"
     CDS             1647..2789
                     /gene="caiA"
                     /locus_tag="ACHLCY_003924"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP0371091.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2918..4135
                     /gene="caiB"
                     /locus_tag="ACHLCY_003925"
     CDS             2918..4135
                     /gene="caiB"
                     /locus_tag="ACHLCY_003925"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP0371092.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4209..5762
                     /gene="caiC"
                     /locus_tag="ACHLCY_003926"
     CDS             4209..5762
                     /gene="caiC"
                     /locus_tag="ACHLCY_003926"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP0371093.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5871..6656
                     /gene="caiD"
                     /locus_tag="ACHLCY_003927"
     CDS             5871..6656
                     /gene="caiD"
                     /locus_tag="ACHLCY_003927"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP0371094.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6662..7252
                     /gene="caiE"
                     /locus_tag="ACHLCY_003928"
     CDS             6662..7252
                     /gene="caiE"
                     /locus_tag="ACHLCY_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP0371095.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7338..7733)
                     /gene="caiF"
                     /locus_tag="ACHLCY_003929"
     CDS             complement(7338..7733)
                     /gene="caiF"
                     /locus_tag="ACHLCY_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP0371096.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7994..11215)
                     /gene="carB"
                     /locus_tag="ACHLCY_003930"
     CDS             complement(7994..11215)
                     /gene="carB"
                     /locus_tag="ACHLCY_003930"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP0371097.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGETGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11233..12381)
                     /gene="carA"
                     /locus_tag="ACHLCY_003931"
     CDS             complement(11233..12381)
                     /gene="carA"
                     /locus_tag="ACHLCY_003931"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP0371098.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2978 a         3299 c         3369 g         3056 t
ORIGIN      
        1 taacgcgtcc acgaggttaa taataattat attaaatgtt aacaaaaata aaacaaacgg
       61 gaacgcaaaa taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa
      121 aaacgggaat agaaccgaag gttttctttc cgccgttaat aatcgtcggc atactttgtt
      181 ggcttacagt cagagatctg gatgcagcga atgtcgttat taatgctgta ttcagttacg
      241 tcaccaatgt atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt
      301 tctggctggt gtttggcccg tatgccaaaa agcgtttagg taacgaacca cctgaattta
      361 gtaccgccag ttggatcttt atgatgttcg cctcctgtac gtctgctgcc gtactgttct
      421 ggggatcgat tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact
      481 cgacaggggc gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt
      541 gggccactta cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg
      601 aagtgattcg ccccagctcg acactggtgc cgctggtagg tgaaaaacac gccaaagggt
      661 tgttcggcac tatcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggtacca
      721 gtctgggcct tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc
      781 ataccctgca actggacgct atcatcatta cctgctggat tatcctcaac gccatttgcg
      841 tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct
      901 tcctgatgct gggttgggtg ttcattgtca gcggtgccag cttcatcatg aactacttca
      961 ccgattcggt ggggatgttg ctgatgtatc tgccgcgcat gttgttctat accgatccca
     1021 tcgctaaagg cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt
     1081 atgctatcca gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac
     1141 tgtgcttcgg catggtgctg gggctgacag cgtcaacctg gatcctgtgg actgtactcg
     1201 gtagtaacac tctgctgttg atagataaaa acatcatcaa cattccaaat ctgatcgaac
     1261 agtacggtgt ggcgcgcgcc atcattgaaa cctgggccgc tctgccactc agcaccgcca
     1321 ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt tacgctggtt aacgcctgct
     1381 cttataccct ggcgatgtcc acttgccgcg aagtacgcga tggtgaagaa ccacctctgc
     1441 tggtgcgtat cggttggtca attctggttg gcattatcgg tattgttctg ctggcgctcg
     1501 gcggcctgaa accgattcaa accgccatta tcgccggagg atgcccgctg ttcttcgtca
     1561 acattatggt gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa
     1621 cccccaaaat atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt
     1681 ttgtcgccgg tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt
     1741 gcgaccgtga cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg
     1801 acagtctgct gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg
     1861 ccgtgtggat ggagctggga cgtctggggg caccaaccta tgtgctgtac cagttgccgg
     1921 gcgggttcaa caccttcctg cgcgaaggca cacaagagca gatcgacaag attatggctt
     1981 tccgcggcac cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg
     2041 acgtgggtag cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta
     2101 gtaagtgttt tattaccagc agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg
     2161 gggcttctcc ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca
     2221 tcaaagtgac caaacttgaa aagctcggtc tgcgtatgga tagctgctgt gaaatcacct
     2281 ttgacgacgt ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc
     2341 gcgtcaaaga agagttcgac catgaacgtt tcctggtagc cctcaccaac tacggtacgg
     2401 cgatgtgcgc ctttgaagat gcggcgcgct acgccaatca gcgcgtgcag tttggcgagg
     2461 ctattggtcg tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact
     2521 ccatgaaaaa catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg
     2581 gcgatgcagc gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtt gtggatagcg
     2641 caatgcaggt gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc
     2701 gtgatctgcg tgtagaccgc gtctccgggg gatctgacga aatgcagatc ctgacgctgg
     2761 gtcgcgcggt gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctaatgc
     2821 gtcttatcag gcctacggga gcacgaatgt aggccggata aagcgtttac gccgcatccg
     2881 gcagtcatgc gctacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa
     2941 attcgggccg ttggccggat tgcgcgttgt cttctccggt atcgaaatcg ccgggccgtt
     3001 cgccgggcaa atgttcgcag aatggggtgc ggaagttatc tggatcgaga acgtcgcctg
     3061 ggccgacacc attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc
     3121 gctgtcgtta aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac
     3181 caccgatatc ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg gcattaccga
     3241 tgaagtactg tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca
     3301 gtacggcacc gaggagtaca ccaatcttcc ggcctataac accatcgccc aggccttcag
     3361 tggttacctg attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc
     3421 cgattacttt tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg
     3481 tgaaaccggt aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg
     3541 ccagtacttc atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg
     3601 taaagatccc tactacgccg gttgcggtct gtataaatgc gccgacggct acatcgtgat
     3661 ggaactggtg ggcattaccc aaattgaaga gtgctttaaa gatattggcc tcgcacatct
     3721 gctcggtacg ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta
     3781 cggcccactg gtagaagaga aactcgatgc ctggctggcg gcacatacca tcgcagaagt
     3841 taaagaacgc tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact
     3901 ggaaagcaat ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg
     3961 tcgcacctgc aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg
     4021 gcgcggaatg ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag
     4081 cgaaaacgac attcaggagt tggtcagcaa aggtctggcc aaagttgagg actaaacgcg
     4141 tggcgtcagt cggcttgcct gactagcgcc cgcccaataa caaacgaaca aaaatggata
     4201 gaggtgcaat ggatatcatt ggcggacaac atctacgtca aatgtgggac gatctggcgg
     4261 acgtttacgg tcataaaacg gcgctgattt gtgaatccag cggcggagtc gttaaccggt
     4321 atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt tatacgctgg
     4381 ggattcgcaa aggcgacaag gttgcactac atctcgacaa ctgcccggaa tttatctttt
     4441 gctggttcgg gctggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttac
     4501 gcgaggaaag cgcgtggatc ctgcaaaata gccaggcgtg cctgctggtg accagtgcgc
     4561 aattctatcc tatgtatcaa cagattcagc aggaagatgc cactcaattg cggcacattt
     4621 gcctgacaga tgtggcactt cccgctgatg atggcgtgag ttcgtttact caactgaaaa
     4681 atcaacaacc tgccaccttg tgctatgcac cgccgctatt gactgacgat acggcggaaa
     4741 ttctcttcac ctccggcacc acctcccgac cgaaaggtgt ggtgattacc cattacaacc
     4801 tgcgcttcgc tggatattac tccgcctggc agtgtgcact gcgtgacgat gacgtctacc
     4861 tgacggtaat gcctgcgttt catatcgatt gccagtgtac tgcggcgatg gcggcgtttt
     4921 ctgccggggc cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc tggggacagg
     4981 tgcagaagta ccgcgccacc attaccgaat gtattccgat gatgatccgt acgttgatgg
     5041 tgcagccgcc ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg ttttatctca
     5101 acttgtcgga gcaggaaaaa gatgcgtttt gtgaacgctt cggcgttcgc ttgctgacgt
     5161 cttatgggat gacggaaacc attgtgggca ttatcggcga tcgccctggc gataaacgac
     5221 gctggccgtc gattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca
     5281 atcgcccgct cccggcaggt gagatcggtg aaatctgtat taaaggcgta ccagggaaaa
     5341 ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgacg
     5401 gctggctaca taccggcgat accggatact gcgacgaaga gggctttttt tatttcgtcg
     5461 atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa
     5521 atatcatcgc cacccatcca aaaattcagg atatcgtggt tgtgggtatt aaagattcga
     5581 ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag
     5641 aggaattttt ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg
     5701 agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat
     5761 aaatgaaaca gcagggaact accctgccag ctaataatca cgctataaaa caatatgcat
     5821 tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga atgagtgaat
     5881 cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgt ccaaaagcga
     5941 atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg
     6001 atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaagttcttt tccgcgggct
     6061 gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggtggat
     6121 ttgcgggatt aaccgaaatt ttcaatctcg acaaaccggt tatcgcagct gtgaacggct
     6181 atgcctttgg cggcggtttc gaactggcgc tggcggcaga ttttattgtt tgtgccgata
     6241 acgccagctt cgccctgccg gaagccaagc tgggcatcgt tcctgacagc ggcggcgtgc
     6301 tgcgtctacc gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg accggcagac
     6361 gaatgggcgc agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg
     6421 aactgatgga taacgcccgc gaactggcgc agcagctggt taacagcgcc ccgctggcga
     6481 ttgcggcgct gaaagagatc ttccgcacca ccagcgaaat gccggtagaa gaagcgtatc
     6541 gctatattcg cagcggcgtg ttgaaacact atccgtcggt tctgcattcg gaggatgcca
     6601 ttgaagggcc gctggcgttt gccgagaagc gcgatccggt gtggaaagga cgttaacaac
     6661 cgtgagctat tacgcctttg aggggttaat tccggtggtt cacccgacgg cgtttgtcca
     6721 tcccagtgcc gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact
     6781 cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca atattcagga
     6841 tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa acggccatat
     6901 cgggcacgga gcgatcctgc atggttgtgt gattggtcgc gatgcattgg tcgggatgaa
     6961 cagcgtgatt atggatggcg cggtcattgg cgaagagagc attgttgccg ccatgagctt
     7021 tgtcaaagca ggctttcacg gcgagaaacg ccagttgttg atgggtacgc cagcccgcgc
     7081 tgtacgcagt gttagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca
     7141 ggatcttgtc gggcgctgtc atgcatcgtt acatgaaaca cagccactga ggcaaatgga
     7201 ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg cctaaacggt gatcttgccg
     7261 gatgcgacct ggttgctgga taagatgcta caacatcgca tctggcaagc aggacgcccg
     7321 gtatcaacga gataaaatta acgacgcata ctctttgaca acatcatctg ccacttctgc
     7381 tcgcggttaa gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact
     7441 aatttatccc ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact
     7501 tttaccagtc gctggcactg gcatccccgt ccttccagct tattagggat catcttaact
     7561 tcgcagctta tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg
     7621 tgttcaatat cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc
     7681 atccattcgg cgattaacaa gtagagtggt ttttcaacat atccttcaca catacgaact
     7741 ccagtttcac aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat
     7801 attaaaattg tggatatatc gatagtatgc aacttcaccg tcgataagtg tgagctaaat
     7861 cctgcttatg tcatcaaaaa tatcgattcc ccaaaagatt tacttaatgg gcaaaaaaaa
     7921 cggtcctcat cagagaaccg tattacttat tcgatcaaat tagcggatga aaaatatccg
     7981 ccatgacacg ctattatttg atctgcgcgt gcatttcctg caccgaaatt actttttcgg
     8041 tcgcgtcggc attcagcgcc atcgcggtag cgaaaccacc gttcagggtg gtgtcataat
     8101 gcactttata ttgcagcgca ctgcgacgga tcacgcggga gtcttcaatc gcacgacggc
     8161 ctgaagtggt gttgatgatg taggtatatt cgccattctt gatgcggtcc tgaatgtgcg
     8221 gacggccttc atgcaccttg ttcaccagac gcggattaat gcccgcttcg cccagcacaa
     8281 tcgccgtgcc gtgggtcgca tccagctcga agccctgttt cagcagtttt gccgccaggt
     8341 ccaccacgcg ttctttatcg ccttcgcgca cggaaagcaa cgcacgaccg tgtttcttca
     8401 tggtggagtt gctgcccagc tgcgctttgg caaacgcttc agcgaaggtg cggcccacgc
     8461 ccatcacttc cccggtggag cgcatttctg gccctaacag cgggtcaaca cccgggaatt
     8521 tattgaacgg cagcaccact tctttcaccg agtagtacgg cgggataact tctttggtta
     8581 cgccctgctc agccagcgat ttgccagcca tcacacgcgc cgccactttt gccagcggta
     8641 cgccggtggc tttggagacg aacggaacgg tacgcgccgc acgcgggtta acttcaatca
     8701 ggtagacttc gttgtttttc accgcaaact gcacgttcat cagaccgcgc acctgcaatt
     8761 cgaaggccag tttctgcacc tgctggcgca tcacatcctg aatttcctga cttaaggtgt
     8821 aggctggcag agaacatgcg gagtcaccgg agtgcacgcc cgcctgctcg atgtgctcca
     8881 tgatgccgcc aatcagcacc atttcgccgt cgcagatggc atccacgtca acttctaccg
     8941 cgtcatcaag gaagtgatcc agcaacactg gcgcatcgtt agacacgctg accgccgtct
     9001 ggaagtagcg acgcaggtca gcttcgtcat agacgatttc catcgcccga ccgccgagaa
     9061 cgtaagacgg acgtaccacc agcgggtagc caatctcttt cgccttctct accgccattt
     9121 caatagcggt aacggtggcg ttcgccggtt gtttcagttt cagacgctca accgcatgct
     9181 ggaagcgttc acggtcttct gcacggtcga tagcatccgg gctggtgccg ataaccggta
     9241 cgccagcagc ttccagcgcg cgcgccagtt tcagcggggt ctgaccgccg tactgaacga
     9301 taacgccttt cggcttctca atacgcacga tttctagcac atcttccagt gttaccggct
     9361 cgaagtagag gcgatcggaa gtgtcgtagt cggtggagac ggtttccggg ttacagttaa
     9421 ccataatggt ttcgtaaccg tcttcgcgca gtgccagcga ggcgtgtaca cagcagtagt
     9481 cgaattcgat accctgaccg atacggttcg ggccgccgcc gagaaccatg attttttcac
     9541 ggtcggtaga cggattcgct tcgcactctt cttcataagt ggagtacatg taagcggtgt
     9601 cggtggcgaa ctctgccgca caggtatcca cgcgcttata aaccgggtgc aggtcatact
     9661 ggtcgcgcag cttgcggatt tccgcttcgc gcacgcccgc cagttttgcc agacgcgcat
     9721 cggcaaagcc tttgcgtttc agctggcgca ggaattcagc gttcaggcca gtgatgccca
     9781 cttccgctac tttctcttcc agacgcacca gttcttcaat ctgtaccagg aaccagcggt
     9841 caatattggt caggttgaac acgccgtcca cagacaggcc cgcacggaag gcatcggcga
     9901 tgtaccagat acgctctgcg ccagcatctt tcagttcgcg acggattttg gttaacgctt
     9961 ccgggtcatc gaggctcact ttcgggtcga atccagtctc accgacttcc aggccgcgca
    10021 gcgctttttg cagggattcc tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt
    10081 tcatctgagt ggtcagacgg tcgttagcac cagcgaattt ttcgaagttg aagcgaggaa
    10141 ttttagtaac cacatagtcg atggacggct cgaaggaggc cggagtacgt ccgccagtga
    10201 tgtcgttcat cagttcgtcg agggtgtaac ccaccgccag tttcgccgcc actttagcaa
    10261 tcgggaaacc ggtcgctttc gacgccagcg ccgaagaacg ggacacgcgc gggttcattt
    10321 cgataacaat caggcgaccg tttttcgggt tcaccgcaaa ctggacattg gaaccaccgg
    10381 tttcaacgcc gatttcacgc agcaccgcca tcgaggcgtt acgcatgatt tgatattctt
    10441 tgtcggtcag cgtttgggct ggcgcgacag tgatggagtc accggtatgg atgcccatcg
    10501 catcaaagtt ttcgatagag cagacgatga tgcagttgtc gtttttatca cgcaccactt
    10561 ccatctcgta ctctttccag ccaatcagcg actcatcaat cagcaactct ttggtcggag
    10621 agagatccag accgcgggcg caaatttctt caaactcttc gcggttataa gcgataccgc
    10681 cgccgctacc gcccatggta aaggatgggc gaataatgca cgggaagccc acgtcagcgg
    10741 caaccgccag cgcttcttcc atcgtgtgtg cgataccgga acgcgcggtt tccagaccaa
    10801 ttttcttcat cgctacgtcg aaacgacggc ggtcttctgc tttatcaatc gcatcggcgg
    10861 tcgcaccaat catagtcacg ccgaactctt ccaacacgcc ctgacgctcc agctccagcg
    10921 cgcagttcag cgccgtctga ccgcccatcg ttggcagcac cgcgtccggg cgctcttttt
    10981 caataatctt gcgtaccact tcccagtgaa tcggctcgat gtaggtcgca tcggccattt
    11041 ccgggtcggt catgatggtc gccgggttgg agttcaccag aatgacccgg taaccctctt
    11101 cgcgcagggc tttacatgct tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa
    11161 caatcgggcc cgcacccaga atcaggatac tttttatatc tgtacgtttt ggcatggctc
    11221 ttttactcct gattacttag cggttttacg gtactgctca attaactcga taaagtggtc
    11281 gaacaacggc gcggcgtcgt gtggaccagg gctggcttca gggtgcccct ggaagctgaa
    11341 cgccggttta tcggtgcgat gaatgccctg taacgtaccg tcgaacaggg atttatgcgt
    11401 gacacgcagg tttgctggta atgttgcttc gtccaccgca aaaccgtggt tctgggcggt
    11461 gatcatcacc acgtttttct caacatcttt aaccggatgg ttgccgccgt ggtgaccaaa
    11521 tttcattttg acagtcttcg caccgctcgc cagcgccagc agctgatgac cgaggcagat
    11581 gccgaagacc ggaatatcgg tttcgaggaa tttctggatg gcggtaatgg cgtaatcgca
    11641 cggggccggg tcgccaggac cgttggagag gaagatgccg tctggattca ttttcagcac
    11701 atcttccgca gaagtttgcg ccggaacgat ggtcaggcga cagcctctgt ccaccaacat
    11761 gcgcaggatg ttgcgcttgg caccaaaatc ataagccacg acgtggaacg gcagctcgtc
    11821 ttcttttttc gcttctggca ggccgccggt caacgtccag ctcccttgtg tccagctata
    11881 ggcttctgcg gtggtcactt cttttgccag atccatgcca ttcagacctg ggaacgcgcg
    11941 ggctttttct aacgccaacg ccgcatccgg gttatcgcct gcgataatgc agccattctg
    12001 tgcgcctttc tcgcgcagta aacgcgtcag cttacgggta tcgatatcgg caatcgccac
    12061 gatgttatgg cgcttcaggt aagaagagag gtcttcggta ttacggaagt tgctggcaat
    12121 cagcggcagg tcgcgaatca ccagaccttg tgcatgtacc tgagaagatt cttcatcggc
    12181 gtcattagtg ccgacattgc caatatgggg ataagtaaga gtaacgattt gacgagaata
    12241 ggaaggatca gtgaggattt cttgataacc ggtcattgaa gtattgaaaa cgacttcccc
    12301 aaccgccgaa cctgttgccc ctatggcccg accgtgaaac tgggttccgt cttccagaac
    12361 caatagcgct gacttaatca aaacaccctc cagagaatat tcactcactt tatttgcata
    12421 ttaattcaca atgatgacgt aaatcaatgc aaatctgctt accgacgaat ttctggcaaa
    12481 cggcggcatt ctggagatat gggcgctaaa agtcaactta atggcgggat tttttatatt
    12541 attttgtggc tcatgcatgt tttacaggca ttaagcggca aaaagatcat ataacctgtt
    12601 ctggaaaacg cttgcgcagc aagaagattg catgaaataa aagcgagtag ggaaaagtgg
    12661 accaaatggt caaaatttac attaaaacaa caaaaaccga ag
//
LOCUS       DAZGTR010000126        12629 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGTR010000126 DAZGTR010000000
VERSION     DAZGTR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.128"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(113..1396)
                     /gene="gltA"
                     /locus_tag="ACHLCY_003932"
     CDS             complement(113..1396)
                     /gene="gltA"
                     /locus_tag="ACHLCY_003932"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP0371099.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1602..1709)
                     /locus_tag="ACHLCY_003933"
     CDS             complement(1602..1709)
                     /locus_tag="ACHLCY_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371100.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2090..2494
                     /gene="sdhC"
                     /locus_tag="ACHLCY_003934"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2090..2494
                     /gene="sdhC"
                     /locus_tag="ACHLCY_003934"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0371101.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2488..2835
                     /gene="sdhD"
                     /locus_tag="ACHLCY_003935"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2488..2835
                     /gene="sdhD"
                     /locus_tag="ACHLCY_003935"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP0371102.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2835..4601
                     /gene="sdhA"
                     /locus_tag="ACHLCY_003936"
     CDS             2835..4601
                     /gene="sdhA"
                     /locus_tag="ACHLCY_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP0371103.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4617..5333
                     /gene="sdhB"
                     /locus_tag="ACHLCY_003937"
     CDS             4617..5333
                     /gene="sdhB"
                     /locus_tag="ACHLCY_003937"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP0371104.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5634..8435
                     /gene="sucA"
                     /locus_tag="ACHLCY_003938"
     CDS             5634..8435
                     /gene="sucA"
                     /locus_tag="ACHLCY_003938"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP0371105.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8450..9667
                     /gene="odhB"
                     /locus_tag="ACHLCY_003939"
     CDS             8450..9667
                     /gene="odhB"
                     /locus_tag="ACHLCY_003939"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP0371106.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9761..10927
                     /gene="sucC"
                     /locus_tag="ACHLCY_003940"
     CDS             9761..10927
                     /gene="sucC"
                     /locus_tag="ACHLCY_003940"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP0371107.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGLMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10927..11796
                     /gene="sucD"
                     /locus_tag="ACHLCY_003941"
     CDS             10927..11796
                     /gene="sucD"
                     /locus_tag="ACHLCY_003941"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP0371108.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11900..12622)
                     /gene="mngR"
                     /locus_tag="ACHLCY_003942"
     CDS             complement(11900..12622)
                     /gene="mngR"
                     /locus_tag="ACHLCY_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP0371109.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3031 a         3216 c         3430 g         2952 t
ORIGIN      
        1 atgaatgtcc gatagtggat tttattttta caattttttg tgtttaggca tataaaaatt
       61 aacccgccat ttgaacggcg ggttaaaata tttacaactt agcaatcaac cattaacgct
      121 tgatatcgct tttaaagtcg cgtttttcat atcctgtata cagctgacgc ggacgggcaa
      181 tcttcatacc gtcactgtgc atttcgctcc agtgggcgat ccagccaacg gtacgtgcca
      241 ttgcgaaaat gacggtgaac atggaagacg gaatacccat cgctttcagg atgataccag
      301 agtagaaatc gacgttcggg tacagtttct tctcgataaa gtacgggtcg ttcagcgcga
      361 tgttttccag ctccatagcc acttccagca ggtcatcttt ggtgcccagc tctttcagaa
      421 cttcatggca ggtttcacgc attacggtgg cgcgcgggtc gtaatttttg tacacgcggt
      481 gaccgaagcc catcaggcgg aaagaatcat ttttatcttt cgcacgacga acaaattccg
      541 gaatgtgttt aacggagcta atttcttcca gcattttcag cgccgcttcg ttagcaccac
      601 cgtgcgcagg tccccacagt gaagcaatac ctgctgcgat acaggcaaac gggttcgcac
      661 ccgaagagcc agcggtacgc acggtggagg tagaggcgtt ctgttcatgg tcagcgtgca
      721 ggatcagaat acggtccata gcacgttcca gaatcggatt aacttcatac ggttcgcacg
      781 gcgtggagaa catcatattc aggaagttac cggcatagga gagatcgttg cgcggataaa
      841 caaatggctg accaatggaa tacttgtaac acatcgcggc catggtcggc attttcgaca
      901 gcaggcggaa cgcggcaatt tcacgatgac gaggattgtt aacatccagc gagtcgtgat
      961 agaacgccgc cagcgcgccg gtaataccac acatgactgc cattggatgt gagtcgcgac
     1021 ggaaagcgtg gaacagacgg gtaatctgct cgtggatcat ggtatgacgg gtcaccgtag
     1081 ttttaaattc gtcatactgt tcctgagtcg gtttttcacc attcagcagg atgtaacaaa
     1141 cttccaggta gttagaatcg gtcgccagct gatcgatcgg gaaaccgcgg tgcagcaaaa
     1201 taccttcatc accatcaata aaagtaattt tagattcgca ggatgcggtt gaagtgaagc
     1261 ctgggtcaaa ggtgaacaca ccttttgaac cgagagtacg gatatcaata acatcttgac
     1321 ccagcgtgcc tttcagcaca tccagttcaa cagctgtgtc cccgttgagg gtgagttttg
     1381 cttttgtatc agccatttaa ggtctcctta gcgccttatt acgtaagact gccggaactt
     1441 aaatttgcct tcgcacatca acctggcttt acccgtttgt tatttggctc gccgctctgt
     1501 gaaagagggg aaaacctggg tacagagctc tgggcgcttg caggtaaagg atccattgat
     1561 gacgaataaa tggcgaatca agtacttagc aatccgaatt attaaacttg tctaccacta
     1621 ataactgtcc cgaatgaatt ggtcaatact tccacactgt tacataagtt aatcttaggt
     1681 gaaataccga cttcataact tttacgcatt atatgctttt cctggtaatg tttgtaacaa
     1741 ctttgttgaa tgattgtcaa attagatgat taaaaattaa ataaatgttg ttatcgtgac
     1801 ctggatcact gttcaggata aaacccgaca aactatatgt aggttaattg taatgatttt
     1861 gtgaacagcc tatactgccg ccaggtctcc ggaacaccct gcaatcccga gccacccagc
     1921 gttgtaacgt gtcgttttcg catctggaag cagtgttttg catgacgcgc agttatagaa
     1981 aggacgctgt ctgacccgca agcagaccgg aggaaggaaa tcccgacgtc tccaggtaac
     2041 agaaagttaa cctctgtgcc cgtagtcccc agggaataat aagaacagca tgtgggcgtt
     2101 attcatgata agaaatgtga aaaaacaaag acctgttaat ctggacctac agaccatccg
     2161 gttccccatc acggcgatag cgtccattct ccatcgcgtt tccggtgtga tcacctttat
     2221 tgcagtgggt atcctgctgt ggcttctggg taccagcctc tcttcccctg aaggtttcga
     2281 gcaagcttcc gcgattatgg gcagcttctt cgtcaaattt atcatgtggg gcatccttac
     2341 cgctctggcg tatcacgtcg tcgtaggtat tcgccacatg atgatggatt ttggctatct
     2401 ggaagaaaca ttcgaagcgg gtaaacgctc cgccaaaatc tcctttgtta ttactgtcgt
     2461 gctttcactt ctcgcaggag tcctcgtatg gtaagcaacg cctccgcatt aggacgcaat
     2521 ggcgtacatg atttcatcct cgttcgcgct accgctatcg tcctgacgct ctacatcatt
     2581 tatatggtcg gttttttcgc caccagtggc gaactgacat atgaagtctg gatcggtttc
     2641 ttcgcctctg cgttcaccaa agtgttcacc ctgctggcgc tgttttctat cttgattcat
     2701 gcctggatcg gcatgtggca ggtgttgacc gactacgtta aaccgctggc attgcgcctg
     2761 atgctgcaac tggtgattgt cgttgcactg gtggtttacg tgatttatgg attcgttgtg
     2821 gtgtggggtg tgtgatgaaa ttgccagtca gagaatttga tgcagttgtg attggtgccg
     2881 gtggcgcagg tatgcgcgcg gcgctgcaaa tttcccagag cggccagacc tgtgcgctgc
     2941 tctctaaagt cttcccgacc cgttcccata ccgtttctgc gcaaggcggc attaccgttg
     3001 cgctgggtaa tacccatgaa gataactggg aatggcatat gtacgacacc gtaaaagggt
     3061 cggactatat cggtgaccag gacgcgattg aatatatgtg taaaaccggg ccggaagcga
     3121 ttctggaact cgaacacatg ggcctgccgt tctcgcgtct tgatgatggt cgtatctatc
     3181 aacgtccgtt tggcggtcag tcgaaaaact tcggcggcga gcaggcggca cgcactgcgg
     3241 cggcggctga ccgtaccggt cacgcactgt tgcacacgct ttatcagcag aacctgaaaa
     3301 accacaccac cattttctcc gagtggtatg cgctggatct ggtgaaaaac caggatggcg
     3361 cggtggtggg ttgtaccgca ctgtgcatcg aaaccggtga agtggtttat ttcaaagccc
     3421 gcgctaccgt gctggcgact ggcggggcag ggcgtattta tcagtccacc actaacgccc
     3481 acattaacac tggcgacggt gtcggcatgg ctatccgcgc aggcgtaccg gtgcaggata
     3541 tggaaatgtg gcaattccac ccgaccggca ttgccggtgc gggcgtactg gtcaccgaag
     3601 gttgccgtgg tgaaggcggt tatctgctga acaaacatgg cgaacgcttt atggagcgtt
     3661 atgcgccgaa cgccaaagac ctggcgggcc gtgatgtggt ggcgcgttcc atcatgattg
     3721 aaatccgtga aggtcgcggc tgtgatggtc cgtgggggcc acacgcgaaa ctgaaactcg
     3781 atcacctggg taaagaagtt ctcgaatccc gtctgccggg tatcctggag ctttcccgta
     3841 ccttcgctca cgtcgatccg gtgaaagagc cgattccggt tatcccaacc tgtcactaca
     3901 tgatgggcgg tattccgacc aaagttaccg gtcaggcgct gactgtgaat gagaaaggcg
     3961 aagatgtggt tgttccggga ctgtttgccg ttggtgaaat cgcttgtgta tcggtacacg
     4021 gcgctaaccg tctgggcggc aactcgctgc tggacctggt ggtctttggt cgcgcggcag
     4081 gtctgcatct gcaagagtct atcgccgagc agggcgcact gcgcgatgcc agcgagtctg
     4141 atgttgaagt gtctctggat cgcctgaacc gctggaacaa taatcgtaac ggtgaagatc
     4201 cggtggcgat tcgtaaagcg ctgcaagaat gtatgcagca taacttctcg gtcttccgtg
     4261 aaggtgatgc gatggcgaaa gggcttgagc agttgaaagt gatccgcgag cgtctgaaaa
     4321 atgcccgtct ggatgacact tccagcgagt tcaacaccca gcgcgttgag tgcctggaac
     4381 tggataacct gatggaaacg gcgtatgcaa cggctgtttc tgccaacttc cgtaccgaaa
     4441 gccgtggcgc acatagccgc ttcgacttcc cggatcgtga tgatgaaaac tggctgtgcc
     4501 actccctgta tctgccagag tcggaatcca tgacgcgccg aagcgtcaac atggaaccga
     4561 aactgcgccc ggcattcccg ccgaagattc gtacttacta atgcggagac aggaaaatga
     4621 gactcgagtt ttcaatttat cgctataacc cggatgttga tgatgctccg cgtatgcagg
     4681 attacaccct ggaagcggaa gaaggtcgcg acatgatgct gctggatgcg cttatccagc
     4741 tgaaagagaa agatcccagc ctgtcgttcc gccgctcctg ccgtgaaggt gtgtgcggtt
     4801 ccgacggtct gaacatgaac ggcaagaatg gtctggcctg tattaccccg atttcggcac
     4861 tcaaccagcc gggcaagaag attgtgattc gcccgctgcc aggtttaccg gtgatccgcg
     4921 atttggtggt agacatggga caattctatg cgcaatatga gaaaattaag ccttacctgt
     4981 tgaataatgg acaaaatccg ccagctcgcg agcatttaca gatgccagag cagcgcgaaa
     5041 aactcgacgg gctgtatgaa tgtattctct gcgcatgttg ttcaacctct tgtccgtctt
     5101 tctggtggaa tcccgataag tttatcggcc cggcaggctt gttagcggca tatcgtttcc
     5161 tgatcgatag ccgtgatacc gagactgaca gccgcctcga cggtttgagc gatgcattca
     5221 gcgtattccg ctgtcacagc atcatgaact gcgtcagtgt atgtccgaag gggctgaacc
     5281 cgacgcgcgc catcggccat attaagtcga tgttgttgca acgtaatgcg taaaccgtag
     5341 gcctgataag acgcgcaagc gtcgcatcag gcaaccagtg ccggatgcgg cgtgaacgcc
     5401 ttatccggcc tacaagtcat tacccgtagg cctgataagc gcagcgcatc aggcgtaaca
     5461 aagaaatgca ggaaatcttt aaaaactgcc cctgacacta agacagtttt taaaggttcc
     5521 ttcgcgagcc actacataga caagagctcg caagtgaacc ccggcacgca catcactgtg
     5581 cgtggtagta tccacggcga agtaagcata aaaaagatgc ttaagggatc acgatgcaga
     5641 acagcgcttt gaaagcctgg ttggactctt cttacctctc tggcgcaaac cagagctgga
     5701 tagaacagct ctatgaagac ttcttaaccg atcctgactc ggttgacgct aactggcgtt
     5761 cgacgttcca gcagttacct ggtacgggag tcaaaccgga tcaattccac tctcaaacgc
     5821 gtgaatattt ccgccgcctg gcgaaagacg cttcacgtta ctcttcaacg atctccgacc
     5881 ctgacaccaa tgtgaagcag gttaaagtcc tgcagctcat taacgcatac cgcttccgtg
     5941 gtcaccagca tgcgaatctc gatccgctgg gactgtggca gcaagataaa gtggccgatc
     6001 tggatccgtc tttccacgat ctgaccgaag cagacttcca ggagaccttc aacgtcggtt
     6061 catttgccag cggcaaagaa accatgaaac tcggcgagct gctggaagcc ctcaagcaaa
     6121 cctactgtgg cccgattggt gccgagtata tgcacattac cagcaccgaa gaaaaacgct
     6181 ggatccaaca gcgtatcgag tctggtcgcg cgactttcaa tagcgaagag aaaaaacgct
     6241 tcttaagcga actgaccgcc gctgaaggcc ttgaacgtta cctcggcgca aaattccctg
     6301 gcgcaaaacg cttctcgctg gaaggcggtg acgcgttaat cccgatgctt aaagagatga
     6361 tccgccacgc tggcaacagc ggcacccgcg aagtggttct tgggatggcg caccgtggtc
     6421 gtctgaacgt gctggtgaac gtgctgggta aaaaaccgca agacttgttc gacgagtttg
     6481 ccggtaaaca taaagaacac ctcggcacgg gtgacgtgaa ataccacatg ggcttctcgt
     6541 ctgacttcca gaccgatggc ggcctggtgc acctggcgct ggcgtttaac ccgtctcacc
     6601 ttgagattgt aagcccggta gttatcggtt ctgttcgtgc ccgtctggac agacttgatg
     6661 agccgagcag caacaaagtg ctgccaatca ccatccacgg tgacgccgca gtgaccgggc
     6721 agggcgtggt tcaggaaact ctgaacatgt cgaaagcgcg tggttatgaa gttggcggta
     6781 cggtacgtat cgttatcaac aaccaggttg gtttcaccac ctctaatccg ctggatgctc
     6841 gttctacacc gtactgtact gatatcggta agatggttca ggcaccgatt ttccacgtta
     6901 acgcggatga tccggaagcc gttgcctttg tgacccgtct ggcgctcgat ttccgtaaca
     6961 cctttaaacg tgatgtcttc atcgacctgg tgtgctaccg ccgtcacggc cacaacgaag
     7021 ccgacgagcc gagcgcaacc cagccgctga tgtatcagaa aatcaaaaaa catccgacac
     7081 cgcgcaaaat ctacgctgac aagctggagc aggaaaaagt ggcgacgctg gaagatgcca
     7141 ccgagatggt taacctgtac cgcgatgcgc tggatgctgg cgattgcgta gtggcagagt
     7201 ggcgtccgat gaacatgcac tctttcacct ggtcgccgta cctcaaccac gaatgggacg
     7261 aagagtaccc gaacaaagtt gagatgaagc gcctgcagga gctggcgaaa cgcatcagca
     7321 cggtgccgga agcagttgaa atgcagtctc gcgttgccaa gatttatggc gatcgccagg
     7381 cgatggctgc cggtgagaaa ctgttcgact ggggcggtgc ggaaaacctc gcttacgcca
     7441 cgctggttga cgaaggcatt ccggttcgcc tgtcgggtga agactccggt cgcggtacct
     7501 tcttccaccg ccacgcggtg atccacaacc agtctaacgg ttccacttac acgccgttgc
     7561 aacacatcca taacgggcag ggcgcgttcc gcgtctggga ctccgtactg tctgaagaag
     7621 cagtgctggc gtttgaatac ggttatgcca ccgcagaacc acgcactctg accatctggg
     7681 aagcgcagtt cggtgacttc gccaacggtg cgcaggtggt tatcgaccag ttcatctctt
     7741 ctggcgaaca gaaatggggc cggatgtgtg gtctggtgat gttgctgccg cacggttacg
     7801 aagggcaggg gccggagcac tcctccgcgc gtctggaacg ttatctgcaa ctttgtgctg
     7861 agcaaaacat gcaggtgtgc gtaccgtcta ccccggcaca ggtttaccac atgctgcgtc
     7921 gtcaggcgct gcgcgggatg cgtcgtccgc tggtcgtgat gtcgccgaaa tccctgctgc
     7981 gtcatccgct ggcggtttcc agcctcgaag aactggcgaa cggcaccttc ctgccagcca
     8041 tcggtgaaat cgacgagctt gatccgaagg gcgtgaagcg cgtagtgatg tgttctggta
     8101 aggtttatta cgacctgctg gaacagcgtc gtaagaacaa tcaacacgat gtcgccattg
     8161 tgcgtatcga gcaactctac ccgttcccgc ataaagcgat gcaggaagtg ttgcagcagt
     8221 ttgctcacgt caaggatttt gtctggtgcc aggaagagcc gctcaaccag ggcgcatggt
     8281 actgcagcca gcatcatttc cgtgaagtga ttccgtttgg ggcttctctg cgttatgcag
     8341 gccgcccggc ctccgcctct ccggcggtag ggtatatgtc cgttcaccag aaacagcaac
     8401 aagatctggt taatgacgcg ctgaacgtcg aataaataaa ggatacacaa tgagtagcgt
     8461 agatattctg gtccctgacc tgcctgaatc cgtagccgat gccaccgtcg caacctggca
     8521 taaaaaaccc ggcgacgcag tcgtacgtga tgaagtgctg gtagaaatcg aaactgacaa
     8581 agtggtactg gaagtaccgg catcagcaga cggcattctg gatgcggttc tggaagatga
     8641 aggtacaacg gtaacgtctc gtcagatcct tggtcgcctg cgtgaaggca acagcgccgg
     8701 taaagaaacc agcgccaaat ctgaagagaa agcgtccact ccggcgcaac gccagcaggc
     8761 atctctggaa gagcaaaaca acgatgcgtt aagcccggcg atccgtcgcc tgctggctga
     8821 acacaatctc gacgccagcg ccattaaagg caccggtgtg ggggggcgtc tgactcgtga
     8881 agatgtggaa aaacatctgg cgaaagcccc ggcgaaagag tctgctccgg cagcggctgc
     8941 tccggcggcg caaccggctc tggctgcacg tagtgaaaaa cgtgtcccga tgactcgcct
     9001 gcgtaagcgt gtggcagagc gtctgctgga agcgaaaaac tccaccgcca tgctgaccac
     9061 gttcaacgaa gtcaacatga agccgattat ggatctgcgt aagcagtacg gtgaagcgtt
     9121 tgaaaaacgc cacggcatcc gtctgggctt tatgtccttc tacgtgaaag cggtggttga
     9181 agccctgaaa cgttacccgg aagtgaacgc gtctatcgac ggcgatgacg tggtttacca
     9241 caactatttc gacgtcagca tggcggtttc tacgccgcgc ggcctggtga caccggtact
     9301 gcgtgatgtc gataccctcg gcatggcaga catcgagaag aaaatcaaag agctggcagt
     9361 caaaggccgt gacggcaagc tgaccgttga agatctgacc ggtggtaact tcaccatcac
     9421 caacggtggt gtgttcggtt ccctgatgtc tacgccgatc atcaacccgc cgcagagcgc
     9481 aattctgggt atgcacgcta tcaaagatcg tccgatggcg gtgaatggtc aggttgagat
     9541 cctgccgatg atgtacctgg cgctgtccta cgatcaccgt ctgatcgatg gtcgcgaatc
     9601 cgtgggcttc ctggtaacga tcaaagagtt gctggaagat ccgacgcgtc tgctgctgga
     9661 cgtgtagtag tttaagcttc acctgcactg tagaccgaca tggatagggt gtcggtctac
     9721 ggtttaaaag ataacgatta ctgaaggatg gacagaacac atgaacttac atgaatatca
     9781 ggcaaaacaa ctttttgccc gctatggctt accagcaccg gtgggttatg cctgtactac
     9841 tccgcgcgaa gcagaagaag ccgcttcaaa aatcggtgcc ggcccgtggg tagtgaaatg
     9901 tcaggttcac gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa acagcaaaga
     9961 agacatccgt gcttttgcag aaaactggct cggcaagcgt ctggtaacgt atcaaacaga
    10021 tgccaatggc caaccggtta accagattct ggttgaagca gcgaccgata tcgctaaaga
    10081 gctgtatctc ggtgccgttg tagaccgtag ttcccgtcgt gtggtcttta tggcctccac
    10141 cgaaggcggc gtggaaatcg aaaaagtggc ggaagaaact ccgcacctga tccacaaagt
    10201 tgcgcttgat ccgctgactg gcctgatgcc gtatcaggga cgcgagctgg cgttcaaact
    10261 gggtctggaa ggtaaactgg ttcagcagtt caccaaaatc ttcatgggcc tggcgaccat
    10321 tttcctggag cgcgacctgg cgttgatcga aatcaacccg ctggtcatca ccaaacaggg
    10381 cgatctgatt tgcctcgacg gcaaactggg cgctgacggc aacgcactgt tccgccagcc
    10441 tgatctgcgc gaaatgcgtg accagtcgca ggaagatccg cgtgaagcac aggctgcaca
    10501 gtgggaactg aactacgttg cgctggacgg taacatcggt tgtatggtta acggcgcagg
    10561 tctggcgatg ggtacgatgg acatcgttaa actgcacggc ggcgaaccgg ctaacttcct
    10621 tgacgttggc ggcggcgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa tcatcctctc
    10681 tgacgacaaa gtgaaagccg ttctggttaa catcttcggc ggtatcgttc gttgcgacct
    10741 gatcgctgac ggtatcatcg gcgcggtagc agaagtgggt gttaacgtac cggtcgtggt
    10801 acgtctggaa ggtaacaacg ccgaactcgg cgcgaagaaa ctggctgaca gcggcctgaa
    10861 tattattgca gcaaaaggtc tgacggatgc agctcagcag gttgttgccg cagtggaggg
    10921 gaaataatgt ccattttaat tgataaaaac accaaggtta tctgccaggg ctttactggt
    10981 agccagggga ctttccactc agaacaggcc attgcatacg gcactaaaat ggttggcggc
    11041 gtaaccccag gtaaaggcgg tactacccac ctcggcctgc cggtgttcaa caccgtgcgt
    11101 gaagctgttg ctgctactgg cgctaccgct tctgttatct acgtaccagc accgttctgc
    11161 aaagactcca ttctggaagc tattgatgca ggcatcaaac tgattatcac catcactgaa
    11221 ggcatcccga cgctggatat gctgactgtg aaagtgaaac tggacgaagc cggcgttcgt
    11281 atgatcggcc cgaactgccc gggcgttatc actccgggtg aatgcaaaat cggtatccag
    11341 ccgggtcaca ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg tacactgacc
    11401 tatgaagcgg ttaaacagac cacggattac ggtttcggtc agtcgacctg tgttggtatc
    11461 ggcggtgacc cgatccctgg ctctaacttt atcgacattc tcgaaatgtt cgaaaaagat
    11521 ccgcagaccg aagcgatcgt aatgatcggt gagatcggcg gtagcgctga agaagaagcg
    11581 gcagcgtaca tcaaagagca tgttaccaag ccagttgtgg gttacatcgc tggtgtgact
    11641 gcgccgaaag gcaaacgtat gggccacgcg ggtgccatca ttgccggtgg gaaagggact
    11701 gcggatgaga aattcgctgc tctggaagcc gcaggcgtga aaaccgttcg cagcctggcg
    11761 gatatcggtg aagcactgaa aactgttctg aaataaatat ctgtaataag aaatagccct
    11821 cgccgcttcc ctctacagga atggcgaagg gctgtcggtt tcgacatggt tggccatcgt
    11881 atgatggcct tttttgtgct tatcgcgatg attttcgttg cgctatcagg gtaaatttat
    11941 agtcatcggt attaaaagcg ttgcggctat attcaaacac ccgaccatca actaaatatc
    12001 cacgcgatac tttttcaaga atcggctttg tctggctgat attaagcaga cggctcatct
    12061 cttcggttgg catcagagga atgatttcct gttcgctacg atcgataacc attttcttca
    12121 cttcttcaat aaagtgatat tttgaatttt ccatgacctg ccaggtgaga tccgggaaca
    12181 acgcaagcgg catccaggtt tcttccagcg ccattggctt ttgcttgcga tagcgcacgc
    12241 gcttcacatg ccacacacga tcctgcgcgg tgatttgtag ctgttgctga agaaaatcgt
    12301 cagccggaat cacttcaaat atcaggactt cactgtgtgt atcgacgtga cggtccgaca
    12361 gtttttcatc aaaactggtt aactgaaaaa tatcgtaatt gacccgctct tctttgacgt
    12421 aagtcccgct gccctgaatg ctttcgagga tctgttgctc gactaactgg cgcaaagcct
    12481 gacgcaccgt aacccggctg acgccaaact ctgtttgtaa tgccgattcg gtaggtaacg
    12541 catcgccagg tttaagctcg ccacgcgcaa tttgttcacg aatgcgatcg gcaatctgcc
    12601 ggtataaggg cttgtgtccc atttttagt
//
LOCUS       DAZGTR010000127        12240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGTR010000127 DAZGTR010000000
VERSION     DAZGTR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.129"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..370
                     /locus_tag="ACHLCY_003943"
     CDS             <1..370
                     /locus_tag="ACHLCY_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Replication protein repL"
                     /protein_id="HFP0371110.1"
                     /translation="PCQDDVAIKNKKSQVKKTKRSVSGGAGTTRLKKLTSWIAEAKAK
                     ADNLRLSKKRAQKHEFKQKVEAAARKYAYLKNKRSPDIGGISNFDNLPHCMTVNEALN
                     AVLAKNKDNEQWGIPAGFRG"
     gene            421..666
                     /locus_tag="ACHLCY_003944"
     CDS             421..666
                     /locus_tag="ACHLCY_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371111.1"
                     /translation="MQSDFLQLAIAFAGYVCIGFCVYMISRKMLVDIDRKERAEEILV
                     WIFFGAVWPLGIMFAATFLLMWIFTLPGDFYRKKARH"
     gene            848..1003
                     /gene="hokD"
                     /locus_tag="ACHLCY_003945"
     CDS             848..1003
                     /gene="hokD"
                     /locus_tag="ACHLCY_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0371112.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(1120..1629)
                     /locus_tag="ACHLCY_003946"
     CDS             complement(1120..1629)
                     /locus_tag="ACHLCY_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HFP0371113.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(1641..2105)
                     /locus_tag="ACHLCY_003947"
     CDS             complement(1641..2105)
                     /locus_tag="ACHLCY_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371114.1"
                     /translation="MNMAQNWEAPFTGMSMGSVSGALGGFVQVGTETTSVARWNSLMV
                     WEGGTPPTFTLPVTFIALNNPFIEVSGAIAALTAMISPELKAANVGGRIPERVTLNIG
                     RRINITDVAIQDLSFDLDAPRDSNGYFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            complement(2258..3073)
                     /locus_tag="ACHLCY_003948"
     CDS             complement(2258..3073)
                     /locus_tag="ACHLCY_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371115.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEQPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDSEVTEEQDDAYNEAWANVADFLAACG
                     VDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEAAFVVAGTSDEMLTEAFKKVV
                     RNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAKLARKKSMKLRRKRLG"
     gene            complement(3083..4672)
                     /locus_tag="ACHLCY_003949"
     CDS             complement(3083..4672)
                     /locus_tag="ACHLCY_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371116.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     GNERFLLKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EELISTAKVTNKKSLAFTGGTNGDQSNISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGKICADRLIDGFFDVKPTLTYAEALPAVEDTGLLGTDYVSCSVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKARGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            complement(4733..6439)
                     /locus_tag="ACHLCY_003950"
     CDS             complement(4733..6439)
                     /locus_tag="ACHLCY_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371117.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTLWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            complement(6665..7666)
                     /locus_tag="ACHLCY_003951"
     CDS             complement(6665..7666)
                     /locus_tag="ACHLCY_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP0371118.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILRKSLDKKPKP"
     gene            complement(7683..8879)
                     /locus_tag="ACHLCY_003952"
     CDS             complement(7683..8879)
                     /locus_tag="ACHLCY_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0371119.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQSSATMF
                     LSHKHSIGIVNATSAQAMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRE
                     LCNEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPP
                     ATVDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVF
                     GGDMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRI
                     EFIRSN"
     gene            complement(9437..10297)
                     /gene="repA"
                     /locus_tag="ACHLCY_003953"
     CDS             complement(9437..10297)
                     /gene="repA"
                     /locus_tag="ACHLCY_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFP0371120.1"
                     /translation="MNQSFISDILYADIESKAKELTVNSNNTVQPVALMRLGVFVPKP
                     SKSKGESKEIDATKAFSQLEIAKAEGYDDIKITGPRLDMDTDFKTWIGVIYAFSKYGL
                     SSNTIQLSFQEFAKACGFPSKRLDAKLRLTIHESLGRLRNKGIAFKRGKDAKGGYQTG
                     LLKVGRFDADLDLIELEADSKLWELFQLDYRVLLQHHALRALPKKEAAQAIYTFIESL
                     PQNPLPLSFARIRERLALQSAVGEQNRIIKKAIEQLKTIGYLDCSIEKKGRESFVIVH
                     SRNPKLKLPE"
     gene            complement(10615..11007)
                     /locus_tag="ACHLCY_003954"
     CDS             complement(10615..11007)
                     /locus_tag="ACHLCY_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371121.1"
                     /translation="MSDKVTVKQTINKATSIYKIEHITVGKPGSEQYRHAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     NGGIGYQFLYEGDDLKCYEHGLLIESVE"
     gene            complement(11019..11159)
                     /locus_tag="ACHLCY_003955"
     CDS             complement(11019..11159)
                     /locus_tag="ACHLCY_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371122.1"
                     /translation="MCRMPEFSSEYFDDLPAYILDTETMLMGINRKNRNVNDYNRAIS
                     GN"
     gene            complement(11185..11607)
                     /locus_tag="ACHLCY_003956"
     CDS             complement(11185..11607)
                     /locus_tag="ACHLCY_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HFP0371123.1"
                     /translation="MSFFSTLKTALSLKEKLAATGVLVLICALVGAGFAWERHQLKQA
                     MEKIGSLDQAVKERDKSIMDLNQTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKA
                     EVQKQLVAAGNVRQRIPADTQRLLRESISEFNADADKG"
     gene            complement(11647..>12240)
                     /locus_tag="ACHLCY_003957"
     CDS             complement(11647..>12240)
                     /locus_tag="ACHLCY_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371124.1"
                     /translation="TTATGLTNIGGLATQHADLLAGYADLSMLLQLDIGWDVYCRANE
                     REVSELPISIAIGDVTITKSLEDAVNALNTSSLVAAMGEINQTLNTGSGSSSGSGSGG
                     GTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERANESANVAITAYNH
                     AIGTALAEASANKASTASAIAALVPDSVLDELNKAAQ"
BASE COUNT         3190 a         2969 c         2851 g         3230 t
ORIGIN      
        1 cccctgtcag gatgacgtgg caataaagaa taagaagtca caagttaaaa aaacaaaaag
       61 atcagtttcc ggcggtgccg gaacgaccag actcaaaaaa ttgacttcat ggatcgctga
      121 ggcaaaagca aaggctgaca atctgcggtt atccaaaaaa cgcgctcaaa aacatgagtt
      181 caagcagaaa gtagaggcgg cagcgcggaa atatgcttac ctgaagaaca agcgttctcc
      241 tgatattggc gggatatcaa acttcgataa tctgccgcat tgcatgacgg taaacgaagc
      301 tcttaatgcg gttttagcca aaaataaaga taacgaacaa tggggtatac cggcaggatt
      361 cagagggtga tagattgctc taatctggag tcacctggcg ttttcagttt gaggtcggag
      421 atgcaatctg attttttaca gttagcgatc gcttttgcag gatatgtttg tattggcttc
      481 tgtgtataca tgatcagccg aaaaatgctt gtcgatatcg accgcaaaga acgagcagag
      541 gagatcttag tatggatttt ctttggcgcg gtctggccat tagggatcat gtttgctgca
      601 acatttcttc tgatgtggat attcaccctt ccaggtgatt tctatagaaa aaaagccaga
      661 cattgataca atcgttgcgg gtgcttgagg ctatctgctt caggcattac ccgaaaagca
      721 gatagaagaa agccccagat aacattacgc gtcctgcaag acgcttaaca ttaatctgag
      781 gccatatcta tgcttagcat acgtagatta gcctcttacc gaccaaaagg tcaaggagaa
      841 gcaggctatg aagcagcaaa aagcgatgtt aatcgctctg atcgtcatct gtttaaccgt
      901 cattgtgacg gcactggtaa cgaggaaaga cctctgcgag gtacgaatcc gaaccggcca
      961 gacggaggtc actgtcttca cagcctacga atctgaaagg taagagacct ggcggggaga
     1021 gatcctcgcc actcttcgtg tgtcaggtat cctcaatgca ccctttcctc tccaaataaa
     1081 aaagctcccg aaggagcttt aaaatacaag ggatgacact taatcccact caatccagtt
     1141 gtagacgata cgaagtgacg ggcgcacagc agcagtcaca tcttcggtac taaagtcgat
     1201 tgcatcactg tagattttgc agtccaacat ttcaattgtt gtagcagctt ttgtcacagc
     1261 gttaaccccg gaagatttgg attcaggggt cgcagccatc gtgatatcaa catagtcctt
     1321 cgccgcaatg cgatctttaa tgaactgaag aatatcgcct tcgatagtct ccacgcactg
     1381 gacctggatt tccccagagt ttcgaattgg accgtgctgg ttgaacttca caccattcgg
     1441 accatagtcc tccacatcct cgcgggtcat ttcaggaatt tgcgacgtgc gaaccagtac
     1501 gctgatatct tcatggcctg caaaagtgag ctggaattca gaagatacca gtcgttcgcc
     1561 tttggccgcg ttggcagtat agcggccctt aataaattta cggtttccct tagtgttatt
     1621 gtgccccata taaaatcctt ttactggaac gcccgaacaa tatcggagct gttatatatc
     1681 gaagaaccgg tcaactggag gttgacggtg tttttcagga aatatccatt actgtccctt
     1741 ggcgcatcga gatcaaaact taagtcctgg atggcgacat cggtgatgtt gatccggcga
     1801 ccaatgttta gcgtcacgcg ctccgggatt cgaccaccaa cattggccgc ttttagttcc
     1861 gggctaatca tggctgtcaa cgcggcgata gcgcctgaaa cttcaatgaa tggattgttc
     1921 aaagcaatga aagttacagg cagcgtgaac gtcggcggag tccccccttc ccaaaccatt
     1981 aagctattcc aacgggcaac cgacgttgtt tcagtaccta cttgcacaaa accacccaga
     2041 gcaccagaaa cagatcccat ggacataccg gtaaacggcg cttcccaatt ctgggccatg
     2101 ttcattgccg ccccctggct gatatatccg gtaacctggt actgagagtt cgttaaagta
     2161 actttcagaa atggcgatac accgtcagcc tggctgtaaa ccccataagg tataggtgcc
     2221 attcaagtta aaggccggag ttctccggcc tcctccttta gccaaggcgc ttacggcgca
     2281 gtttcattga ctttttgcgg gcaagttttg ccgcgccggt ctgggctttt cgacgcgctt
     2341 ttttcagcgc cgatttttga gccgcagtca gacgtttttt acgcaggcgt ttacggatga
     2401 gtttgatctc accgttacga acaaccttct taaatgcttc agtcagcatt tcatcagaag
     2461 tgccagcaac aacaaacgcc gcttccagtt cgtcacggtc gtcgctatct aaaccagcga
     2521 tagaggcacc aacatcagca gctgcgtcgt cgtcttcatc gtcagccagt gcttcgatca
     2581 ggtcatcatc tacaccgcat gctgcgagga agtcagcaac atttgcccat gcttcgttat
     2641 aggcatcgtc ctgttcttct gtaacttcgg agtcgtcgtc atcagagata ccagcgatag
     2701 cctgaacgaa accatcaagg gagtcgaaag tcagatcacc gctatcagcc caggcgaaaa
     2761 cggcgtcggc cgcatcactc aacgcattct gcatagcact tctatttgca gcttccagaa
     2821 tcatctggtg cgcctgttcg acggtccatt ctttaccgtc tttcccttcc aggatttgct
     2881 caggagccgg ggcagatgga acgttatcgt tagtctgtgc cgccggttcc ggattattat
     2941 taataaccgg atctgttggc tgttcggcgc ttgctcgggc agactccatc agctgcacag
     3001 gatcagagtt caaagcgaaa cgagacagtc cattccccaa aaatgccccg gattgaaaaa
     3061 agtttttgct cattgtattc ccttacttaa taagcagcgg tacgccctgg atacgacggg
     3121 ctacgccagt cgggcagcag gcccagacta cttcccattt atcgaattcc gcctgcgtaa
     3181 ctttcagcac atacggttct gtaccgtcag catcaggatc acgaggagcc accagagcgc
     3241 cggaggcgac aaagcgatct aaaagtttgg tcatcccttt agtcaggcca gccgcagtaa
     3301 taccgtccgg gctatgcttc atctgtcggg ctaactggac aaagaaacgg ctgattgcat
     3361 tcatcaggga tgggacgtgc tggaagtgca gatagttatc ctgcgtgcag caagttaaag
     3421 catcgtcgat gatcatctgg ccagaggtgc caacagatac tttattgaga cggcccttga
     3481 ccattgcttc ttcgtccggg gtatcttccg gatacagcgg ttgaattgac gcacgagcaa
     3541 tgacggcacg ttcttcacca gccggtgagt aatgccaacc gccgacatca gagtttttct
     3601 tgacgccacg agctttcgcc gcatacgcca cgccagacag accaaagacc acacgggatt
     3661 gggtccattt gtctttgcag gagaacgggt agtgatagac agaacagctt acataatcgg
     3721 taccaagtaa accggtatcc tcaacagctg gtagtgcttc tgcgtacgtt aatgtcggtt
     3781 tgacatcaaa gaagccatca atcaggcgat ctgcacagat tttacctaat gcggtgatag
     3841 ccgcattgtc atagcagccc aggccgagaa cagcggtgta catgtacggc gcattgttca
     3901 gcactttcac cgcacgcagg taagcagcag tggaaatgtt cgactgatcg ccgttggtac
     3961 cgccagtgaa cgccagcgat tttttatttg ttactttcgc cgtcgaaatc agctcttcat
     4021 taacaaccgc gcgcagatat ttagaacggg cttccagagc cgtaggcaga taacacaagc
     4081 ggcccatgtc atctttcgct tcttccgcca aagacacagt gtgtgtctcc agggtcgtta
     4141 ccacaccgag cgaagtcgtc tgggtcagtt ttaagaggaa gcgttcatta cccgcgctgt
     4201 ccgctgttgc cgtttcgatg gttaactcac gggtaggaga aatacacgga tcaccatcat
     4261 caacgtagat agcaaaggct tcgccgctat caagttcaat ttcagaaccg tatggcaacg
     4321 cactgtaagc cggttcgcct gattcatcga acataataat cgggaacttc gcatcatccg
     4381 gaacagcacg aacaacataa ccagacgttt gctgaatagc ttcgtataca tggcgaattg
     4441 gttcgaactg tgagccggaa gacggcttca gcggttcgcc gagaacatct tcgtaattgg
     4501 actcagtaac cgcaagaaca gtaaacggct tgccacgcgc aaatacgcca ataccagccc
     4561 acaagctgct atttaatgca acaccggtag ataacgtcgc atcggcattg atcgggctaa
     4621 ccgcgacgcc ggatgcatta cctaatgact gttgaattga atattgagac ataactttcc
     4681 ctgttatgcg ccccgcacgg gggcgctatg ttaaacggag aacttcccct gattactcag
     4741 agtcaccggc atcaatcgtg tcgccgctta tgaagttaag cccgcctttt ttggccattg
     4801 tcagcgttac acgagtgaag taatcagcgc cgttgcgtgg gtgcatatcg ttgatagccg
     4861 aaccccacag cgtggtacgg ttgaccagcg ccggagtggt cggatgctgg aacgggatgg
     4921 ccgggacagc atcaccagtc acgaagcctg ctttacccgg attttcatca cggacgtagc
     4981 acagcacatc catcgagctg aactgaatgt tctctgtcgt taagttctta caaataccag
     5041 caggtacttc gtacactttc acgttaccga acagggtacc gatgtagtga acatacggag
     5101 tctggatata gtcttcggct ggctggaaga aatccttcgg caactgtttg aagaaagatg
     5161 ctgcatcagc accagcaaac atccccatcg caccagaaga tttaacgcgc tcaataatgt
     5221 cgcgatatac agtctggaat ttgccacgaa tgatggttgc ccatacatca aaggactggt
     5281 taaccggcag agcgatgtca aaggtgtcgg tcgcaagagt gcgccagatc atgatgcgaa
     5341 gacgtagcat atcctgttca tgagacaggt attccttcag ggtgcggaac tgtagggaac
     5401 ccaggtccag accaaattca cgctgtgctt catacgccgc ctgtaccgtg tgctcagccg
     5461 cgataacgaa ctgacttggg aacagggtgt atttcttcat ttcgtggttg atcagcggga
     5521 tcagctcagg agcggcttca atattgattt ccgtctcaat tgcgatctca gtgcctttat
     5581 ccggcgcttt ggagaacgac agggcaatct gaccaatgtt gtagttcaga gagcaggtaa
     5641 cagtgatttg ctcaccagca gcattagtaa acgagtgaag taggctgccg gaaccattat
     5701 caacaacaga cttaatacgg ttaacgtaga tgttagtgcg accttttcgg attggtacat
     5761 tctggccttc gaagtcttcc atcttgaagg ttgcggtttt gctagtgcca tcggagcttg
     5821 ccaccagcac atagcggcga cgtaactggc tgtacacacc gacggattgc atgtccagaa
     5881 catcaccagc agcataagaa ccaaaagagg aacctgccac gttaaagact tcatagatgt
     5941 cggactggtc acgcgtaacc ggaatgaagg tacacgcatc agcggtagct gcccccaact
     6001 gaacaggcag gatcatcgcg aggaataaag gcagacgcat aacaccgtca gaaacgctca
     6061 tcatctctgc tgcgacggac tccagcatcg ctttattagt ggcatccatg ctattgcggg
     6121 tggactcaat caggcagttt tccagcgtct ggtggcagga ggccagaatt tccggacgcg
     6181 gcatagattt atgtgctgcg gcgtagtcag ccagtgcact tgcccacgct gtagcgattt
     6241 gagcggtggc attatcagag atacccgcaa aaaccgggtc tttacgtgca gcttcaagga
     6301 tagatgcggc acgcgcggca tcatctttaa tgaattggtt atcagtaccg aactgcgcag
     6361 tgcttgccca gccaagcaca gctttagagc gttttgcgat atctgcaata cgattctggt
     6421 attcgcgtaa gttactcaat ttactcttcc ttaaacacaa ggcacttgtg tgaatccctt
     6481 ttcggaagag attttattga aagtcacttg ttgactttct cgtgacaaga aattttttta
     6541 tttttttcgg gaagagggga gaaaggtaaa atccaaggtg aaatcgtggc gatttcacct
     6601 tgaaatttta taagatttta ttttaaaaac agtaagttaa tggtgaaatt tgaatggcga
     6661 aagtttaagg cttcggcttt ttatcgaggc tctttctaag gatatgccca atcatcctgt
     6721 cgagttcttc ctgtaactct tttgagagtc gattaaactc ataagaaaat gcacggcctt
     6781 tcacgcgctt ccttgcaaag cgatccttgt cctcaaattt ccataattca gtaactacgg
     6841 acttatcttt agaaccttta tccgtgagta gtgaggcttc ctttgttatc aagcgcagga
     6901 ttttattttt aacttcatct tcggccattt cttcaatgga taagatgtcg tttatttccg
     6961 gggatatgtt ttgaataagc tgatcaaact ctaaattctt gttccccatt tcgtcgccaa
     7021 cagcacaaag cgttttgtag tccgaaaagg ttaattccga ctgcacaggg aaaagggcga
     7081 ctaattcttc cggagcactc gctgcctgga gagcacgcgt gaccttcgcc tgagatagcc
     7141 cttctttggc tgcaatatcc ttctgactca tcccatcatt tttcattcgc atcaaacgca
     7201 gacctatttc tcgaatgctg tgctgcaatg ctgtctgaac gtctttcgct aaattttgcg
     7261 cttcctggac gctgatctcc tggtccgtga ctaaaacccg caaccctacg ttctctaaga
     7321 tggcagaagc tcgacgccgg gaaccatcca aaatttcaat tttccctgta gcacgtctaa
     7381 cacctattgc agggtaaaat tgctgatgct taatagtgct tcggatactt tttaatgatt
     7441 ttggcgtaag agatgcctgg tcacgcccgt tgttatgctg atcaacaaag gtatcgcttt
     7501 ctacctggtt cggaggtatt acctcttcaa taaatgtggc ctgacgacca gttgataact
     7561 tgaatacctg ctcgactcga tcgccagagg ctgaagaact atcaaatccg cttaatattg
     7621 aaggattaag ggttcgccca attgttggtc tgtttttctt tgacatgggg gtttcttact
     7681 cctcagttag atctgataaa ttcaatacgg tcaaacactg cttttgcaaa atcttccgcg
     7741 gcaattcgcg cgttcttcaa tgcatcagca ctaccaacat acgttgccgg gttagctgaa
     7801 ataacagtgt caaaagactc gccgcagcgt tcaaaaccgt caaggcgagg gaggacgaca
     7861 tcaagcatat ccccaccgaa cacttcttta gccaggctat ggcaatattt atggtctgcc
     7921 ttgttactca acttggacat aaaaccaatg ttagtcgcaa gctggcactc gcagccttca
     7981 tccgaaatga gtttcaccaa ctcaggaagg cgggcaacgt atttaagcga tgagtggaaa
     8041 tcaaccgttg caggcggcag aggtgtaaac agtatattgg ccgaggccaa agcatttttc
     8101 aggaaggcgt caaggtgagg accactatca acgaggataa agtcataatc gctcttcagc
     8161 ttatcaatca cattttcttt caggactgca tggatgttct gacccggtag atgctcattg
     8221 cacagctctc tccaatcgga tgcaataaag gcatcgtcaa tcgacgcagg cataacgtca
     8281 accccaggta caacagaagg aacaataaac tcctctaaca gctcttcacg gcttacattc
     8341 tgcaacatag cctgtgcaga tgttgcgttt acgataccaa tagagtgttt atggcttaaa
     8401 aacatcgttg ctgaagattg cggatcaagg tcaataacca gaatccttaa atcttccatc
     8461 agaagatgag ggtgagcacg cattgcatgc gccagagaaa ccgtcgatac agtttttgac
     8521 acaccgcctt taagattgga gatgaaaatc acatacgctt cgctgtagcg atcccggtat
     8581 tttggcactc cgcgatgttc atatatgtca atgatgttct gaattgacat cgcatatttc
     8641 attgaagagc cagcagggcg tttatcgaaa acataaccct tttcttccat ttcacttacg
     8701 gcatagtcaa cgttcgctcg agtcagtaga ggcaattttg ccagtgccgc tttcgcatag
     8761 acctggtaaa actcgttcgc gtgtagctca tccttttgca actgtacttg ttcagtcaga
     8821 acattgagca ttctgtttgc tctttgagca accttgtgaa gctggctgga atcactcatc
     8881 gaaagtcatc ctttatgctg tatttttgaa tttaattaaa aatgctgcat aaaataataa
     8941 tgtatgcgta gatgcttgta catagcattc cctgcatgtt tactcctttg ccaagtgtgt
     9001 cacaacatgc tttggcatcc tccacgcata aaggacgcgg atttcttcta tgttcaggtt
     9061 gtagcctgaa tcattttggt gatttcctgc ttcaacgggc agcccggctt cactctctat
     9121 caactggtga gaacggcttg cccggcagct tcgatgtgtg ctggagggag aataaaagtc
     9181 taagatgtgc gatagaggga agtcgcattg aattatgtgc tgtggaggga tcgctggtat
     9241 caaatatgtg tgctggaggg aaagacagag aattacatgt gcactggagg gaaaaacgga
     9301 tggacagatg tgtgctggag ggaaagtctg agcaagctgc ggggcgtccc cctccagcgc
     9361 acatcaaaaa cgggaaattg gacaagcctt cccggcagca cacatttttt gaatgcagct
     9421 gccctccagc acacacttat tcggggagtt tcagctttgg attgcgagaa tggacgatta
     9481 caaaactttc ccggcctttc ttctcaattg aacagtcgag atagccgatt gttttaagct
     9541 gttctatcgc tttcttaatg atacggtttt gctcgccaac agctgactgc aaagccaggc
     9601 gctcacggat tcgcgcgaac gatagcggca acgggttctg cggaaggctt tcgatgaaag
     9661 tgtaaatggc ttgtgcagct tctttcttcg gaagggcacg caaggcgtgg tgttgcaaca
     9721 gaacgcgata atcaagctgg aacagctccc acagcttaga atcagcctcc agctctatca
     9781 gatcaaggtc agcatcaaaa cgcccgacct tcagcagacc agtctgatag ccgcctttag
     9841 catcttttcc gcgcttaaaa gcgataccct tgttacgcaa gcgtccaagt gattcatgaa
     9901 tggttaaacg cagtttcgca tccagacgtt ttgaggggaa accacaggct ttagcgaatt
     9961 cctgaaacga taactggatg gtgtttgagg acaagccgta tttgctgaac gcgtagatga
    10021 caccgatcca cgttttgaaa tcagtatcca tatcgagtcg aggaccggtg attttaatat
    10081 catcgtaacc ctcggcttta gctatctcca gctgggaaaa cgctttggtg gcatcaatct
    10141 ctttactttc tcctttgctc tttgatggct tcggcacgaa tacccccaag cgcatcaacg
    10201 ctacaggctg cacagtgttg tttgaattaa ctgttagttc ttttgcctta ctttcaatgt
    10261 ctgcgtaaag aatatcggag ataaaagatt gattcatatt actttttccg aattatgtgg
    10321 atagttttta taagcggtga taactacccg ggctttcccg tcagcacaca tcctatatcc
    10381 cgccagcaca cattagcaac ccgtcagcac acatttttat cccgtcagca cacatcgttt
    10441 tccctccagc acacatcgcg atacacttct aagccagacg tggcgcggcc tgcaacgatc
    10501 agggatctat atggatctaa ttgggatctg tatggaccta attattggat ctacccagtg
    10561 gataatgtgg ataagtgaaa aaccggccaa catagccggt tggaaggaag ggtattattc
    10621 tacgctttcg ataagaagac catgttcata acatttaaga tcatcgcctt cgtacaggaa
    10681 ttggtatcca ataccaccat tttcatggac attagggaat aactcataac tcactgaaga
    10741 gcaaatcaca ccaatgcaac gatcaacgcc ttctcgttct tcagtgctga aaaaatcctc
    10801 ttcggtaaga acatgagtac attgctcatc agcataggtc ggaaatacat gctcgatgca
    10861 atccgggtgt tttaaaccaa gctgatcggc aagctcgaaa gcatgacggt attgttcaga
    10921 tcctggcttg ccaacagtga tgtgctcaat tttgtagatt gaagtcgctt tgttgatagt
    10981 ttgctttact gttactttat cagacataaa aatccctttt agttaccgct gatagcgcgg
    11041 ttgtaatcat taacgttgcg attcttcctg ttaatcccca tcagcatcgt ttctgtatcg
    11101 aggatatacg ctggcagatc atcaaaatat tcactgctaa actctggcat cctgcacata
    11161 aatgcacttt ttggggcagg gtggttaacc tttgtcggcg tcggcgttaa attcgctgat
    11221 cgactcccgg agcaaccgct gagtgtcagc aggaatacgc tggcgaacat tacccgccgc
    11281 aacaagttgt ttctgaactt cagcttttcg ttccatttgc ctgtcagcat acttggcttg
    11341 ttctgattca tttttcactt cctggctgtg aaaatgttgc tctgctttgt tcatcgtctc
    11401 aatggtctgg ttaagatcca ttattgactt atcacgttcc ttaacagcct gatcaagact
    11461 gccaattttc tccatggctt gctttagctg atgacgttcc cacgcaaacc cagcaccaac
    11521 aagtgcgcaa atcagaacaa gaacaccagt agcagcaagt ttctccttca aagacaaagc
    11581 tgtttttaat gtagaaaaga atgacatgtc ttcctcctga agaaaaatta tcaatgaagt
    11641 cctttgttac tgtgccgctt tgtttaattc atcaagaaca gaatcaggaa ccaaagcggc
    11701 gattgcgctg gctgtgctgg ccttatttgc tgatgcttcc gcaagcgcgg taccgatagc
    11761 atggttataa gcagttatgg ctacgttggc gctttcattc gctcgttcat actgctgttg
    11821 taacgcagtt gtgggtgctg ttgtctggtt gaaaacaacc ccaaactgtt cagttgctac
    11881 tttcagagat tcaatttgct cttctgttag tgctggtggg ggagtggcag tgccgccgcc
    11941 tgaaccagag cctgacgagc ttcctgagcc agtgttaagg gtctggttaa tctcccccat
    12001 agcagcgact aaacttgatg tattaagcgc gtttacagcg tcctcaagcg atttagtaat
    12061 agtcacatca ccaatggcaa tagagatcgg cagttctgaa acttctcgct cattagcacg
    12121 acagtaaaca tcccaaccaa tatcgagttg aaggagcatt gacagatcag cataaccagc
    12181 caacaggtcc gcgtgctgag ttgccagccc tccaatattc gttaaaccgg ttgcggttgt
//
LOCUS       DAZGTR010000128        12223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGTR010000128 DAZGTR010000000
VERSION     DAZGTR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.130"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1165)
                     /gene="ydcO"
                     /locus_tag="ACHLCY_003958"
     CDS             complement(<1..1165)
                     /gene="ydcO"
                     /locus_tag="ACHLCY_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP0371125.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1257..1793
                     /gene="sutR"
                     /locus_tag="ACHLCY_003959"
     CDS             1257..1793
                     /gene="sutR"
                     /locus_tag="ACHLCY_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP0371126.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1866..3827
                     /gene="rlhA"
                     /locus_tag="ACHLCY_003960"
     CDS             1866..3827
                     /gene="rlhA"
                     /locus_tag="ACHLCY_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP0371127.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3919..4149)
                     /gene="yncJ"
                     /locus_tag="ACHLCY_003961"
     CDS             complement(3919..4149)
                     /gene="yncJ"
                     /locus_tag="ACHLCY_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP0371128.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4371..4547
                     /gene="hicA"
                     /locus_tag="ACHLCY_003962"
     CDS             4371..4547
                     /gene="hicA"
                     /locus_tag="ACHLCY_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP0371129.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4593..5009
                     /gene="hicB"
                     /locus_tag="ACHLCY_003963"
     CDS             4593..5009
                     /gene="hicB"
                     /locus_tag="ACHLCY_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP0371130.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5088..6494
                     /gene="ydcR"
                     /locus_tag="ACHLCY_003964"
     CDS             5088..6494
                     /gene="ydcR"
                     /locus_tag="ACHLCY_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0371131.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLVQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6739..7884
                     /gene="ydcS"
                     /locus_tag="ACHLCY_003965"
     CDS             6739..7884
                     /gene="ydcS"
                     /locus_tag="ACHLCY_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP0371132.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7902..8915
                     /gene="ydcT"
                     /locus_tag="ACHLCY_003966"
     CDS             7902..8915
                     /gene="ydcT"
                     /locus_tag="ACHLCY_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0371133.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8916..9857
                     /gene="ydcU"
                     /locus_tag="ACHLCY_003967"
     CDS             8916..9857
                     /gene="ydcU"
                     /locus_tag="ACHLCY_003967"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0371134.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9847..10641
                     /gene="ydcV"
                     /locus_tag="ACHLCY_003968"
     CDS             9847..10641
                     /gene="ydcV"
                     /locus_tag="ACHLCY_003968"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0371135.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10663..12087
                     /gene="patD"
                     /locus_tag="ACHLCY_003969"
     CDS             10663..12087
                     /gene="patD"
                     /locus_tag="ACHLCY_003969"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP0371136.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGEVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2955 a         2996 c         3275 g         2997 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaca ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtcgcgctca ttatgcagcg cctgatacaa actgccgcta atggtactta
      181 acagcgccag acctgccagc atctggatcc aacttacggg cagggcagcc atcatcccgg
      241 taatagcact accaaacaga cctgcgatca aataaaaaat gcctgcaacg gcagctgcca
      301 gccaacgttg atctttatcc ggatgcgctt ccgggctttg gcaaatagcc gcggtgattg
      361 ccgcaatacc gacggaataa acgccgaaag gggaaaaaac cagtgccagc aatccagtaa
      421 atacaattaa tggcgaaaca ggagccgaat acccggctgc tttcattgct gcgatacccg
      481 gtgcgttttg cgatgccatc gtcaccagaa aaagggggag tgcaacgctc aggctgtgag
      541 caaacgaaaa atcaggggta atataagtgg ggagaacggg tttaaagaca acatcagttg
      601 tgacaacgtc accttgcgcg ataacgatca cgatcccaat aatcatcgcg gcaattaccg
      661 catagcgcgg cgcaacggcc ttggttgcca gccataccag caacatactt ccacacaacg
      721 taaattgacc gtccagactg gcaaacgcct gtaaaccaaa gcgtaataaa atcccggcaa
      781 gcattgccgc cgcaagcgag tgcggaataa tgcgcatcag acgagcaaag agtcccgtta
      841 tgccgcagag gactattaac gcgttggtga caataaaaac gccgatggct tcgttaagtg
      901 ttagtccctg caatccggtg accaacaaag ccgcgccagg cgttgaccat gcggtgagaa
      961 caggtacgcg ataccataat gtcagagtca gcgtactgac gcccattgcc agccccagcg
     1021 ccgtcatcca gccagagatt tgtgcagtgg tggctccggc gacaatcgct gcttgccaga
     1081 ttattgccgc tgaactggcg tagccaatta ataccgccag aaaccccgcc agtagcgtgg
     1141 gtggaggaat agaaaacaga cgcatagtca cctcgtgcgt tatagcgtac aggcaccgta
     1201 gcatttgtcc gttataacgc acaagtgata aacttccgtt ttgccggagg agtcgcatgg
     1261 aaaatctcgc tcgcttttta tccaccacac ttaaacaact acgccagcag cgcggctgga
     1321 gtctttcgcg actggcagaa gcgacgggcg tttctaaagc aatgttaggg cagattgagc
     1381 gtaatgagtc cagtccgacg gtcgcgacat tatggaaaat tgccaccggg ttgaacgtac
     1441 cattttcaac atttatttct ccgccgcagt ccgctacgcc ttcagtttat gatccacaac
     1501 agcaggcgat ggtgatcact tcgctgtttc cttatgatcc acaactctgc ttcgaacact
     1561 tctcgattca gatggcatct ggtgcaataa gcgaatcgac gccccatgag aagggggtaa
     1621 tcgaacatgt cgtcgtcatt gatggacaac ttgatctgtg cgttgatggg gaatggcaga
     1681 cccttaattg tggggaaggc gttcgatttg ctgcagacgt cacgcatatc tatcgtaatg
     1741 gtggggagca aaccgtacat tttcattccc tcatccatta cccgcgcagt taagcgggaa
     1801 aactatttcg caacgtcgcg cttgtggcta aaatagccgc catttttcag ctactggata
     1861 agaatgtgac cgtatcttct catcgacttg aactgttaag cccggcacgc gatgccgcca
     1921 ttgcccgcga agctattttg cacggtgccg atgctgttta tatcggcggc cctggttttg
     1981 gtgcccgtca taatgccagt aatagcctga aagatattgc cgagctggtg ccgtttgccc
     2041 atcgttatgg tgcaaaaatt ttcgtcacgc ttaacaccat tttgcatgat gatgagctgg
     2101 aacccgcgca acggctgatt accgacctct accagaccgg tgtcgatgcg ctgattgttc
     2161 aggatatggg gattctggaa cttgatattc cgccgattga actgcacgcc agtacgcagt
     2221 gcgacattcg tacagtagaa aaagcgaagt tcctctctga tgttggcttc acgcagattg
     2281 tgctggcgcg agagctgaat cttgagcaga tccgcgcgat tcaccaggcc acggacgcga
     2341 ccattgaatt ctttattcat ggggcactgt gcgtggccta ttcgggtcag tgctacattt
     2401 ctcatgcgca aacagggcgt agcgccaacc gtggtgattg ctcgcaggcg tgccgtttgc
     2461 catacacatt gaaagacgat caggggcggg tggtttccta tgaaaaacat ctgctgtcga
     2521 tgaaagataa cgatcagact gccaacctcg gcgcgctgat tgatgctggc gtacgctcct
     2581 tcaagattga agggcgttac aaagatatga gctacgtgaa gaatatcacc gcccattatc
     2641 gccagatgct tgatgccatt attgaagaac gtggcgatct ggcgcgcgct tcatcaggtc
     2701 gtactgaaca tttctttgtt ccatcgacgg aaaagacttt ccaccgtggt agcacagatt
     2761 attttgtgaa tgcccgtaaa ggcgatattg gcgcgttcga ttcgccgaaa tttatcggcc
     2821 tgccggtagg cgaagtattg aaagtggcga aagatcatct cgatgttgcc gtcaccgagc
     2881 cactggcaaa tggtgatggc ctgaacgtga tgattaaacg tgaagtcgtc ggttttcgtg
     2941 ccaatacggt cgagaaaacc ggagaaaatc agtaccgcgt ctggcccaat gaaatgccag
     3001 cagatttgca caaaattcgt ccacatcacc cactaaaccg taatcttgat cataactggc
     3061 agcaggcact gacaaaaacc tccagcgaac gtcgggtggc ggtagacatt gaactgggcg
     3121 gctggcagga acaactgatt ctgaccctca ccagtgaaga gggtgtcagc atcacgcata
     3181 cgctggacgg gcagttcgac gaagccaata acgccgaaaa agcaatgaac aatctgaagg
     3241 atggtctggc aaaactgggg caaaccctct attacgcccg cgatgtgcaa attaatttgc
     3301 cgggggcgct gtttgtacca aacagtctgt taaaccagtt ccgccgtgaa gctgctgaca
     3361 tgctggatgc tgcgcgtctt gccagttacc agcgcggcag ccgtaaaccg gttgctgatc
     3421 ctgcgccggt ttatccgcaa acgcatctga gtttcctcgc gaacgtatac aaccagaaag
     3481 cgcgtgaatt ttatcatcgc tatggtgtgc agctgattga cgcggcgtat gaagcacatg
     3541 aagagaaggg cgaagtcccg gtgatgatca ccaagcattg tctgcgcttt gcctttaatc
     3601 tgtgcccgaa acaggcgaaa ggcaatatca aaagctggaa ggcgacgcca atgcaactgg
     3661 ttaacggcga tgaagtatta acgctaaagt ttgattgccg cccatgcgag atgcacgtca
     3721 ttggcaaaat caaaaatcac atactgaaaa tgccgttacc gggaagcgta gtggcatccg
     3781 taagtccgga tgagctgctg aaaacattgc ctaagcgaaa agggtaaaac gccagttttc
     3841 tggttactca caactcattg aatctgcatg atattgcctg ccgggtaagg cgtctcgccg
     3901 catccggcaa caaatgactc aacgttgtcc cgcttctggt ttgcgcgatt tttgccagta
     3961 aaaatgctcg cgcaaacctt ccgccgactc ttccgccaca gcacgcaatt catcgctgtc
     4021 cgcttcatga cgcagctgat gatccacatt ctttacccac acaaattcat gtcctttgtg
     4081 ccctgccatg agttgtcctg agaacagcgc acacgttaat aagacaaccg ataacgcctt
     4141 cgtaaacatc ctgccacctt tttcttacct tttgccgcta tgatgccgat cgtttcttga
     4201 ggttattatt cagttttgca aattagcgca aagaaattct ggaatcttcc ttcctgattt
     4261 tgcatggcat tctgccgttg cggcgattta gtgctatttt cgagcaaatt acacacggag
     4321 gtaaacacta atgtttactt tgttgatata ctcagcggca gggaggcgat gtgaaacaaa
     4381 gcgagttcag acgttggctc gaatctcagg gcgtcgatgt agcgaatggc agcaaccatt
     4441 tgaaactcag gtttcatggg aggcgcagtg tcatgccgcg tcacccctgc gatgagatta
     4501 aagaaccatt gcgtaaagca atcctgaaac aactcggttt gagttaatcg ccaattaaaa
     4561 aggttaatga catgcgaggg acagtcgaaa ttatgcgtta tcccgtcact cttacacccg
     4621 cgccggaagg cggttatatg gtttcttttg tggatatccc tgaagcgttg acgcagggcg
     4681 aaactgtcgc tgaagcgatg gaagcggcaa aagatgcttt actgaccgca tttgattttt
     4741 attttgaaga taacgagctt atcccgttac cttcgccatt aaatagtcat gatcacttta
     4801 ttgaagtacc tttgagcgtc gcctctaaag tattgctgtt aaatgctttt ttacagtcag
     4861 aaatcactca gcaagagtta gccaggcgaa ttggcaaacc taaacaggag attactcgcc
     4921 tatttaactt gcaccatgcg acaaaaatcg acgccgtcca gcttgcggca aaggcgcttg
     4981 gcaaagagtt atcgctggtg atggtttaat tacagttaac gaaaagttgt catttttaac
     5041 aactgatata gactgccgaa tcatctgcac ataattacga ttcgataatg aaaaaatacc
     5101 agcagcttgc agaacaatta cgcgagcaga ttgcgtcggg tatctggcaa cccggcgatc
     5161 gtttgccttc gttgcgtgac caggtggcgc tttcaggcat gagctttatg actgtcagcc
     5221 atgcctatca gttgctcgaa agtcagggat atattatcgc acgaccgcag tcgggttatt
     5281 acgttgcgcc acaggcaata aaaatgccga aagcgccagt cattccagtc actcgagatg
     5341 aagcagtcga tatcaacact tatatttttg atatgttgca ggccagtcgc gatccgtcgg
     5401 tcgttccgtt tgcctcggcc tttcccgacc cgcgactttt ccccctccaa caactaaacc
     5461 gctcgctggt gcaggtaagc aaaaccgcca cggcgatgag cgtgattgaa aacttaccgc
     5521 caggaaacgc agaactgcgt caggctattg ctcgtcgcta tgccttacag ggcatcacca
     5581 tttctcctga tgaaattgtc attactgccg gggcgttaga ggcattaaac ctcagtttgc
     5641 aagcggtaac tgaaccgggc gattgggtga tagtagagaa tccttgtttc tatggcgcgt
     5701 tgcaggcgct ggaacggcta cggctgaaag cgttatcggt ggcgacggat gttaaagaag
     5761 ggatagatct tcaggcgttg gaactggcgt tgcaggagta tccggtgaaa gcgtgctggc
     5821 tgatgactaa tagccagaat ccactcggat ttaccttaac gccgcaaaaa aaagcacaac
     5881 tggtggcgtt actcaatcag tacaacgtaa cgctgattga agatgacgtt tacagcgaac
     5941 tttattttgg acgggaaaaa ccgctgcctg cgaaagcgtg ggatcgccac gatggcgttt
     6001 tgcattgctc ttcgttttcg aaatgtctgg tgcctggttt tcgtattggt tgggtcgccg
     6061 ccggaaaaca tgcacgtaaa attcaacgct tgcagttgat gagtacgctt tccaccagct
     6121 caccgatgca gcttgcgctg gtggattacc tttccacgcg ccgatacgac gcccatcttc
     6181 gtcgcctgcg tcgccagctt gcggaacgta aacaacgtgc ctggcaggca ctgctgcgtt
     6241 atctgcctgc ggaagttaaa attcatcata gtgacagcgg ttattttctc tggctggagc
     6301 tccccgagcc gttagatgcc ggcgaattaa gcctggcggc actgacgcat catatcagta
     6361 ttgcgccggg taaaatgttt tctaccggtg aaaactggtc acgttttttt cgttttaata
     6421 ccgcgtggca gtggggagaa cgtgaagaac aggcggtaaa acaattaggc aaacttattc
     6481 aagaacggct gtaatagcgt ttaatttaat tcctcttaga ttgggtaata tgaatttcga
     6541 atagcagtca tatttcctaa ccccttgtct atactccaga agataacctt acagacggca
     6601 taatgcgcgg tagctcacaa cctgattaaa ttttctcagg ggcgaaggtg tgcctgcaag
     6661 ccgccgtcta tggttaaaca aggagatatt tttacggcac ggcggctgaa caattaatta
     6721 cgacaggagt aagaccttat gagcaagaca tttgcccgca gcagcctgtg tgcgctcacc
     6781 atgacaataa tgaccgctca cgccgccgaa ccacctacca atttagataa accggaaggg
     6841 cgactggata ttatcgcctg gccgggatac atcgaacgcg gacaaactga taaacaatac
     6901 gactgggtaa cgcaatttga aaaagagaca ggctgcgcgg tgaatgtgaa aaccgccgcg
     6961 acttccgatg aaatggtcag tctgatgacc aaagggggtt acgatctggt tacggcatcc
     7021 ggcgatgcct cgctgcgttt gattatgggc aaacgcgtgc agccgattaa taccgccttg
     7081 attcccaact ggaaaacgct cgatccgcgc gtggttaaag gcgactggtt taatgttggc
     7141 ggcaaagttt acggcacacc ttaccaatgg gggccgaacc tgctgatgta caacactaaa
     7201 accttcccga cgccgccgga tagctggcaa gtggtttttg ttgagcaaaa tctgccggac
     7261 ggcaagagca ataaaggccg cgttcaggct tatgatggcc ctatctacat tgcggacgct
     7321 gcgttgttcg ttaaagccac tcagccgcag ttgggcatca gcgatccgta tcaactaacc
     7381 gaagaacagt accaggcggt gctgaaagtg ctgcgcgctc aacacagttt gatccatcgc
     7441 tactggcatg acactaccgt gcaaatgagc gatttcaaaa acgagggtgt ggttgcttcc
     7501 agtgcctggc cctatcaggc caacgccctg aaagccgaag gccagcctgt tgctaccgtt
     7561 ttcccgaagg agggtgttac cggttgggct gataccacca tgctgcatag cgaagcgaaa
     7621 catccggttt gcgcctacaa atggatgaac tggtcattaa cgccaaaagt gcagggcgat
     7681 gtggcggcct ggtttggctc gttaccggta gtgccggaag ggtgtaaagc cagtccgtta
     7741 ttaggcgaaa aaggttgtga aaccaacggt tttaactatt tcgacaaaat cgccttctgg
     7801 aaaacgccta tagcagaagg gggcaagttt gttccctaca gtcgctggac gcaggattac
     7861 attgccatta tgggcggtcg ctaacttcgc tggggtgctt tatgacgtac gcagtggagt
     7921 ttgacaacgt ctcgcggttg tacggtgacg tgcgcgcagt agatggcgtc agtattgcga
     7981 taaaagatgg tgagttcttc tctatgctgg ggccgtccgg ctccggcaaa accacctgcc
     8041 tgcgcctgat tgctggcttc gaacagcttt ccggcggggc tatctctatc tttggtaaac
     8101 ccgccagcaa tctgccaccg tgggagcggg acgtgaatac tgtctttcag gactacgcgc
     8161 tatttccgca tatgtcgatt cttgacaatg tcgcctatgg gctgatggtc aaaggcgtga
     8221 ataaaaagca gcggcacgca atggcgcaag aggcgctgga gaaagtggcg ttggggtttg
     8281 tacatcaacg taaaccgtca caactttctg gtggtcagcg ccagcgggtt gctatcgcca
     8341 gagcattggt gaatgaaccg cgcgtattgc tgttggatga accgctcggc gcactggatc
     8401 tcaaattgcg tgagcagatg cagctggaac tgaaaaaact gcaacagtct ctcggtatca
     8461 cttttatctt cgttacccac gatcagggcg aagcgttatc gatgtccgat cgtgtggcgg
     8521 ttttcaataa tggacgcatt gagcaggtcg attccccgcg cgatctctat atgcgcccgc
     8581 gcacgccgtt tgttgccgga ttcgtcggga catccaacgt ttttgatgga ctgatggcag
     8641 agaaactttg tggcatgacg ggaagcttcg ccctgcgacc ggaacatatc cgcctcaaca
     8701 cccctggtga actgcaggcc aatggcacga tccaggcggt gcaatatcag ggcgcggcaa
     8761 ctcgttttga actgaaattg aacggcggtg aaaaactgct tgtgagtcag gccaatatga
     8821 caggcgaaga actgcctgcc acgctcacgc ccggacaaca ggtgatggtt tcctggtcgc
     8881 gtgatgtgat ggtgccgctg gttgaggaga ggtgaatggc gatgaatgta ttgcaatcac
     8941 cttcacgtcc aggtctgggt aaggtgtccg gtttcttctg gcgtaatccg gggctggggc
     9001 tgtttttact gctgcttggc ccgctaatgt ggtttggcat tgtctatttc ggctcgctgc
     9061 tgacactgtt atggcaggga ttttatactt ttgacgattt caccatgtcg gtaacgccgg
     9121 aactgacgct ggcgaatatc cgtgcgctgt ttaatccggc gaattacgac atcattctcc
     9181 gcacgctgac tatggctgtg gcagtcacta tcgccagcgc cattctggct tttccaatgg
     9241 cgtggtatat ggcgcgctat accagcggga aaatgaaagc ttttttttat attgcggtaa
     9301 tgttgccgat gtgggcgagc tacattgtta aagcctatgc ctggacgtta ttgctggcaa
     9361 aagatggcgt ggctcagtgg tttttacaac atcttgggct ggaaccactg ctgactgcgt
     9421 tccttacatt acctgcggtg ggcggaaata cgctgtcaac ttccgggctg gggcgctttc
     9481 tggtgtttct ctatatctgg ttgccgttca tgatcctgcc cgttcaggcg gcgcttgagc
     9541 gtttgccgcc gtcattgttg caggcgtcgg ctgatctcgg cgcacgtcca cgacaaacct
     9601 ttcgctatgt ggtgctgccg ctggcaatcc cgggtattgc cgctggctct atctttacct
     9661 tctcactcac actgggcgat tttatcgtcc cgcagctggt tggtcctcca ggatatttta
     9721 tcggcaatat ggtttattcc cagcaggggg cgattggcaa tatgccgatg gcggcggcat
     9781 tcaccctggt gccgattgtt ctcatcgcac tgtacctggc gttcgtgaaa cgtctgggag
     9841 cgttcgatgc actctgaacg cgcaccgttt ttcctcaaac tggcggcctg gggcggcgtg
     9901 gttttcctac attttcccat cctgattatc gccgcttatg cgtttaacac tgaagatgcg
     9961 gcgtttagtt ttccaccgca gggcctgacg ctgcgctggt ttagcgtggc agcacagcgt
    10021 agtgatattc ttgatgccgt gacactgtca cttaaagtgg cggcgctggc gacattaatg
    10081 gcgctggtgt tagggacgct ggcagctgcc gcgctgtggc gacgagactt tttcggcaaa
    10141 aacgccattt cgctgttact gctgctgccc attgcgctgc cgggcattgt cactggtctg
    10201 gcgctattaa ccgcttttaa aaccatcaat ctggagccgg gatttttcac catcgtggtc
    10261 ggtcatgcga ctttttgcgt agtggtggtg tttaacaatg tcatcgcccg ttttcgccgc
    10321 acctcgtgga gtctggttga ggcgtcaatg gatcttgggg ccaatggctg gcaaaccttc
    10381 cgctacgtag tgttgccgaa tctcagttcg gcgctactgg caggaggaat gctggcgttt
    10441 gccttgtcgt tcgatgaaat catcgttacg acttttacgg caggtcatga acgaacgtta
    10501 ccgttgtggt tgctcaatca gcttgggcga ccgcgtgatg taccggtaac taacgtggtg
    10561 gcactgctgg ttatgttggt aacaaccttg ccgatcctgg gggcctggtg gctaacccgc
    10621 gaaggcgaca atggtcaata accactgata caggaatatg ctatgcaaca taagttactg
    10681 attaacggag aactggttag cggcgaaggg gaaaaacagc ctgtctataa tccggcaacg
    10741 ggggaggttt tactggagat tgccgaggca tccgcagagc aggtcgatgc tgctgtgcgc
    10801 gcggcagatg cagcatttgc cgaatggggg caaaccacgc cgaaagtgcg tgcggaatgt
    10861 ctgctgaaac tggctgatgt tatcgaagaa aatggtcagg tttttgccga actggagtcc
    10921 cgtaattgtg gcaaaccgct gcatagtgcg ttcaatgatg aaatcccggc gattgtcgat
    10981 gtttttcgct ttttcgcggg tgcggcgcgc tgtctgaatg gtctggcggc aggtgaatat
    11041 cttgaaggtc atacttcgat gatccgtcgc gatccgttgg gggtcgtggc ttctatcgca
    11101 ccgtggaatt atccgctgat gatggcagcg tggaaactgg ctccggcgct ggcggcaggg
    11161 aactgcgtag tgcttaaacc atcagaaatt accccgctga ccgcgttgaa gttggcagag
    11221 ctggcgaaag atatcttccc ggcaggcgtg attaacgtac tgtttggcag aggcaaaacg
    11281 gtgggtgatc cgctgaccgg tcatcccaaa gtgcggatgg tgtcgctgac gggctctatc
    11341 gccaccggcg agcacatcat cagccatacc gcgtcgtcca ttaagcgtac tcatatggaa
    11401 cttggtggca aagcgccagt gattgttttt gatgatgcgg atattgaagc agtggtcgaa
    11461 ggtgtacgta catttggcta ttacaatgct ggacaggatt gtactgcggc ttgtcggatc
    11521 tacgcgcaaa aaggcattta cgatacgctg gtggaaaaac tgggtgctgc ggtggcaacg
    11581 ttaaaatctg gtgcgccgga tgacgagtct acggagcttg gacctttaag ctcgctggcg
    11641 catctcgaac gcgtcagcaa ggcagtagaa gaggcgaaag cgacagggca catcaaagtg
    11701 atcactggcg gtgaaaagcg caagggtaat ggctattact atgcgccgac gctgctggct
    11761 ggcgcattac aggacgatgc catcgtgcaa aaagaggtat ttggtccagt agtgagtgtt
    11821 acgcccttcg acaacgaaga acaggtggtg aactgggcga atgacagcca gtacggactt
    11881 gcatcttcgg tatggacgaa agatgtgggc agggcgcatc gcgtcagcgc acggctgcaa
    11941 tatggttgta cctgggtcaa tacccatttc atgctggtaa gtgaaatgcc gcacggtggg
    12001 cagaaacttt ctggttacgg caaggatatg tcactttatg ggctggagga ttacaccgtc
    12061 gtccgccacg tcatggttaa acattaagtt agatgttagt ggctgttata ggacggccac
    12121 tatcgtttta ggttctgagt ttaatggcgg ttaagtatta gaatgattac attaataact
    12181 agcgtaaaaa ttaggctaaa tgtttgatgg cgatttttca tcc
//
LOCUS       DAZGTR010000129        12137 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGTR010000129 DAZGTR010000000
VERSION     DAZGTR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.131"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..462
                     /locus_tag="ACHLCY_003970"
     CDS             <1..462
                     /locus_tag="ACHLCY_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP0371137.1"
                     /translation="SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRG
                     NAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            731..2968
                     /gene="nrfB"
                     /locus_tag="ACHLCY_003971"
     CDS             731..2968
                     /gene="nrfB"
                     /locus_tag="ACHLCY_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP0371138.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMFNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRSLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2955..5927
                     /gene="nfrA"
                     /locus_tag="ACHLCY_003972"
     CDS             2955..5927
                     /gene="nfrA"
                     /locus_tag="ACHLCY_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP0371139.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSTEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAAQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5928..6818
                     /gene="ybcH"
                     /locus_tag="ACHLCY_003973"
     CDS             5928..6818
                     /gene="ybcH"
                     /locus_tag="ACHLCY_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP0371140.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7001..7762
                     /gene="envY"
                     /locus_tag="ACHLCY_003974"
     CDS             7001..7762
                     /gene="envY"
                     /locus_tag="ACHLCY_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP0371141.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKVKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8275..9228
                     /gene="ompT"
                     /locus_tag="ACHLCY_003975"
     CDS             8275..9228
                     /gene="ompT"
                     /locus_tag="ACHLCY_003975"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP0371142.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKLPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9415..10899
                     /locus_tag="ACHLCY_003976"
     CDS             9415..10899
                     /locus_tag="ACHLCY_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0371143.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKRENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFIAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(11083..11295)
                     /locus_tag="ACHLCY_003977"
                     /pseudo
     CDS             complement(11083..11295)
                     /locus_tag="ACHLCY_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            11445..12113
                     /locus_tag="ACHLCY_003978"
     CDS             11445..12113
                     /locus_tag="ACHLCY_003978"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0371144.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT         3322 a         2962 c         2864 g         2989 t
ORIGIN      
        1 agtgaaaaga gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat
       61 gaacttattg atttcacgtt tgaatggaaa ggtctgaaga aattatgcgt ggcagtctcc
      121 tttcggtcaa taatagcaga acaaaagaaa gagcccgaaa tgacggtcag atattatatc
      181 agttctgctg atttaaccgc agagaagttc gccacagcaa tccgaaacca ctggcacgtg
      241 gagaataagc tgcactggcg tctggacgtg gtaatgaatg aagacgactg caaaataaga
      301 agaggaaacg ccgcagaatt attttcaggg atacggcaca tcgctattaa tattttaacg
      361 aatgataagg tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga
      421 aactatctcg cgtcagtcct tgcggggagc gggctttcgt aatcttgccc tgcctgctca
      481 cctcctccag gcacttccac tacattatga cctgctcggt gccaccgctc cttttcacta
      541 tgacgttaac ctcccctcct tgacaccatg cttttgtcta cattctacgc aaacggcaac
      601 ttttataacc agtgtaaaaa taacgtgccg caatagtcat acctattaat ggtaaaaagc
      661 tgtcacaatt cataaaaaac cttaatatac gccaccctaa acataaccag cgttaatgta
      721 aggtttttgt gtggactggc ttcttgatgt ttttgcaacc tggctctacg gcttaaaagt
      781 aatcgcgata acgttagcgg tcatcatgtt catcagcggg ctggacgatt tttttattga
      841 tgtcgtctac tgggtacgcc gcattaaacg caagttgagt gtttatcgcc gctacccgcg
      901 aatgagttac cgcgaactgt ataaaccaga tgaaaaaccg ttagcgatta tggttccggc
      961 gtggaatgaa acgggcgtca tcggcaatat ggccgagctg gcggcgacca cgctcgacta
     1021 cgaaaactat catatctttg ttggcaccta ccccaacgac cccgatactc aacgtgatgt
     1081 tgacgaagtg tgcgctcgct ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc
     1141 caccagcaaa gccgactgtc tgaacaacgt gctggacgcc atcacccagt ttgaacgtag
     1201 cgccaatttc gcttttgctg gttttattct gcatgacgcc gaagatgtga tttcaccgat
     1261 ggaattgcgt ctgttcaact atctggtcga gcgtaaagat ctgattcaga tcccggtgta
     1321 tccgttcgaa cgcgaatgga cgcacttcac cagcatgact tacattgatg agttttcaga
     1381 gctgcatggc aaagatgttc cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg
     1441 cgtcggcacc tgtttcagcc gccgcgccgt gaccgctctg ttagctgacg gtgacggtat
     1501 tgctttcgac gtgcagagtc tgactgaaga ttacgacatt ggtttccgcc tgaaagagaa
     1561 aggtatgacg gaaatttttg tccgttttcc ggtggtggac gaagccaaag aacgcgagca
     1621 gcgtaaattt ttacagcacg cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc
     1681 cgataccttt tcgactgcag ttcgacaaaa atctcgctgg atcatcggca ttgttttcca
     1741 gggctttaaa acccacaaat ggacctctag cctgacgctg aactactttc tctggcgcga
     1801 ccgcaaaggg gcaatcagta actttgtcag cttcctcgcg atgctggtga tgctccagct
     1861 tttgctgttg ctggcgtatg aaagtttgtg gcccgatgcc tggcatttcc tttctatttt
     1921 tagcggcagc gcatggttaa tgaccctgct gtggctaaac tttggcttga tgtttaaccg
     1981 catcgtgcag cgggtgattt tcgtcactgg ctactacggt ctgacgcagg ggctgctatc
     2041 tgtcctgcgt cttttctggg gcaacctgat taacttcatg gccaactggc gcgcgctaaa
     2101 acaggtactt caacacggcg atccacgtcg cgtcgcgtgg gataaaacaa cgcatgactt
     2161 ccccagcgtg actggcgata cccgctcgtt gcgctcgtta ggccaaattc tgctggaaaa
     2221 tcaggtcatc actgaagaac aactcgatac agcactgcgt aatcgcgtcg aaggtctacg
     2281 cctgggcggt tcaatgctga tgcaggggct gattagcgcc gagcagctgg cacaggcgct
     2341 ggcagagcaa aacggcgtgg cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct
     2401 gattgccgaa atgccagcct ccgtagcgct gcattatgca gtactgccgc tgcgtctgga
     2461 aaatgatgag ttaattgtcg gcagtgaaga tggtattgac cctgtttcgc tggcggccct
     2521 gacgcgtaaa gtcggacgca aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac
     2581 ggggttacgt cactggtatg cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa
     2641 tgcggttcag catcagtggc tcacggaaca gcagaccggt gaaatctggc ggcaatatgt
     2701 gccgcatcag ttcctgttcg ccgaaatact gaccacgctc ggtcatatta atcgttcagc
     2761 aattaacgtg ttgttattgc gccatgaacg cagttctctg ccgctcggca agtttttggt
     2821 caccgaaggc gttatcagcc aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt
     2881 acaagtttcg atgcaatcac tattactcaa agcaggttta aacacagaac aggttgcgca
     2941 actggagtcc gaaaatgaag gagaataacc ttaatcgcgt catcggatgg tctggtttac
     3001 tgctgacgtc tttattgagt accagcgcac tcgcagacaa tatcggcacc agcacagaag
     3061 agctggggct gagcgattat cgccattttg ttatttatcc ccgtctcgat aaggcgctga
     3121 aggcacagaa aaataacgac gaagcaaccg ccatccgcga atttgaatat atacaccagc
     3181 aggtgccgga taatattccg ctgactttat accttgcgga agcctatcgc cattttggtc
     3241 atgatgaccg ggcgcggctg ttgctggagg atcaactgaa acgtcaccca ggagatgccc
     3301 gacttgagcg cagtctggcg gctattccag ttgaagtgaa aagcgttacg actgttgaag
     3361 aactgcttgc ccagcaaaaa gcgtgcgatg ctgcgccgac cctgcgttgt cgcagtgaag
     3421 tcgggcagaa tgccctgcgg ctggcacagt tacctgtcgc cagagcgcaa ctgaacgatg
     3481 cgacgtttgc tgcatcgccg gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa
     3541 tctacctgaa acaatggtcc caggcagata cgctatacaa tgaagcacgc cagcagaaca
     3601 cattaagcgc ggcagaacgc cgtcagtggt ttgacgtgct tcttgccggg cagctggacg
     3661 atcggatcct ggcactgcaa tcacagggga tcttcaccga tcctcagtca tatattactt
     3721 acgcgaccgc gctggcttat cgtggcgaaa aagcacgcct ccagcattat ctcattgaaa
     3781 ataagccgct gtttaccacg gacgcacaag agaaaagttg gctctatctg ttatctaaat
     3841 acagcgccaa ccccgttcag gcattggcga attatacggt gcagtttgcc gataaccgcc
     3901 agtatgttgt tggcgcgacg ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc
     3961 aaaaactgct cgccaccctc cccgccaatg aaatgcttga ggagcgttat gctgtcagcg
     4021 tggcgacccg taacaaggct gaatctctgc gtctggcacg attgctgtat cagcaagaac
     4081 cggcaaatct tacccgcctg gatcaactga cctggcaact gatgcagaac gagcagtcac
     4141 gcgaagctgc cgatttattg ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc
     4201 agactttaat ggcgcgactg gcgtctctgc tggaaagtca tccttacctg gcaactccag
     4261 cgaaggtggc gattttatca aaacccttgc cgctggcggc gcaacgtcag tggcaaagcc
     4321 agttgccggg tattgcagat aattgcccgg caatagttcg cttgctgggc gatatgtcgc
     4381 cttcctacga tgccgccgcc tggaaccgtc tggcaaagtg ttatcgggac acgctacccg
     4441 gtgtggcctt gtatgcatgg cttcaggccg aacaacgaca accgaacgcc tggcaacatc
     4501 gtgcggtagc ctatcaggcg tatcaggttg aggactacgc caccgcactg gcggcctggc
     4561 agaaaatcag tcttcacgac atgagcaatg aggatctgct tgctgctgcc aataccgccc
     4621 aggcggcagg aaatggtgca gctcgcgatc gctggctaca acaggcagaa caacgtggac
     4681 tgggaaacaa tgccctctac tggtggctgc atgcgcaacg ttacattcct ggtcagccgg
     4741 aactcgcact gagcgatctc acgcgctcaa tcaatattgc gccttctgcc aacgcttacg
     4801 ttgcgcgggc gacaatttat cgccaacgtc ataatgtccc ggcggcggtg agtgatttgc
     4861 gcgccgcgct ggaactggaa ccgaataata gcaacatcca ggcagcgctt ggttacgcct
     4921 tgtgggatag cggtgatatc gcacagtcgc gggaaatgct cgaacaggcg cataaagggc
     4981 ttccggacga tccggcactg atccgacaac tggcctacgt gaaccagcgt ctggatgaca
     5041 tgcctgcgac gcagcactac gcccggctgg tgattgatga cattgataat caggcgctga
     5101 taaccccact gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg
     5161 aggtcggtcg ccgctggacg ttcagtttcg attcttctat cggcttgcgt tccggggcaa
     5221 tgagcaccgc taacaataat gtcggcggcg cagcgccagg gaaaagctat cgtagctacg
     5281 ggcaactgga agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct
     5341 cagtttatag ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa
     5401 atccgatgtc cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg
     5461 ccgtcgaaca gcagttgccg ctgaacggcc aaaatggcgc atccgatacc atgctgcgcg
     5521 ccagcgcctc attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag
     5581 gctggtttgc ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg
     5641 cgtggacggc agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg
     5701 agccttacgc tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc
     5761 ttggcggagt cggggtccgc tggaatatct ggaccggcga gacgcactac gacgcctggc
     5821 cgcacaaagt cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta
     5881 acggagagcg caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca
     5941 ttttcgtatt gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta
     6001 tctggcaacc acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga
     6061 gtcagttacg tttgcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg
     6121 cattcaccca gccagaacag cgcgcgttat tgtttaagcg tgccgcagct gcgcaacagg
     6181 ctggcctgaa gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac
     6241 agtcgtccgc agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag
     6301 ccagattatg gagcgccgcg cctggcgtaa cgccggatgg ctggtacatc agcgcggaaa
     6361 ttgacgacct gaactggcgc agcgaagctg cccgtcagcc tttgctaaca tggttaaaca
     6421 acgcgcagcg gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg
     6481 ccggaaacat gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggcg
     6541 ttaatgtctg ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac
     6601 gttatctaca ggccagcgcc gattgccaaa gtcccgcccc tgccagcggc attgtttatg
     6661 aactgtttgt cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gatgcagaga
     6721 tcgcctcgct gttagcgaaa cgttcctctt gcggtaaaga cactctctat ttctctctgc
     6781 gctatttgcc cgtcgcgcac ggcattctcg agtattaaat ctcctccagg taagtcgggt
     6841 acgacctggc ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag
     6901 cgtgctaaag aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata
     6961 gatcctttga gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga
     7021 accttgcgtg gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac
     7081 gtttaccctt ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact
     7141 ctcaacgtta aatcacgtat taatccccca catcaaccgt aacatcatca acgattatct
     7201 gttgttttta aataagaact taacctgtgt aaagccctgg tcgcggctgg caaccccggt
     7261 tatcgcttgt catagccgta caccggaagt gttccggcta gccgccaacc acagcaagca
     7321 gcaacccagc aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc
     7381 taactttctt gagcaatcgc ggtttatagc cctactgatg tatatcttac gcagcagcgt
     7441 ccgcgacagc gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat
     7501 tgtcgccagt tcgctatgtt taagccccag cctactcaaa aagaaagtaa aaaacgaaaa
     7561 taccagctat agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt
     7621 gatggataac aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta
     7681 ctttatctct gtttttaagg cgttttacgg cctgacaccg ttgaattatc tcgccaaaca
     7741 gcgacaaaaa gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa
     7801 gaagaaatgg cgcgccctgc aggattcgaa cctgcggccc acgacttaga agttcctaga
     7861 acgacatttt aagtcaacaa attaccgcgc catctctgcg ctcacacgtc ccactacctc
     7921 aaaacatgta aagccttgca agccattgtg aggccttatg tgtctcagtt ttgtccctct
     7981 tttttgtact aaaaaacata gcaattgagg ataaacctca tgctattttc gcttatatgc
     8041 ctttaaaggc atggcgctta aatagataaa aacaccacaa aagcataaaa aaccacacag
     8101 taaaaccgca acataaaaca ataacagata attaaaccaa aaacagatag cgcattgtga
     8161 taatcattca atactaaaca aaatataaac agtggagcaa tatgtgattg actcattaag
     8221 ttagatataa aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg
     8281 gcgaaacttc tgggaatagt tctgacaacc cctattgcga tcagctcttt tgcttctacc
     8341 gagactatat cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc
     8401 ggaaaaacaa aagagcgcgt ttatctagct gaagaaggag gccgaaaagt cagtcaactc
     8461 gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
     8521 cagatatcta ttggggctgc gggctggact actctcggca gccgaggtgg caatatggtc
     8581 gatcaggact ggatggattc cagtaacccc ggaacctgga cagatgaaag taggcaccct
     8641 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac
     8701 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
     8761 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
     8821 ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaactgcc ctacattggc
     8881 ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc
     8941 tgggtggaag catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc
     9001 agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca
     9061 cctaacgcaa aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat
     9121 acttcacttt atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata
     9181 gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca
     9241 gattttctcc ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtaggc
     9301 aatatccact aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa
     9361 cacaagacct caatatattc aaatcttatc tttattccga tattcagaga cacaatgcga
     9421 aaaataatta ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc
     9481 cagcagatac agtggcaatc ctgtatggcc agtcaattca accactggtt tggtgaggaa
     9541 aaaccgtctc ctgacttact atgtggttat ttgtctgttc cattaaaata tacagacaca
     9601 ggcggagatg cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg
     9661 gcaaaaagca agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc
     9721 ataaatcctt atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt
     9781 attggatttg atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca
     9841 gatacagaga ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc
     9901 tgtatccata ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac
     9961 gatattgatc gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg
    10021 tatggaactc aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc
    10081 gttcttgatg gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa
    10141 caggcacaga gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa
    10201 agttgcccgc tttcttcaga cagagataag gcaataactc agttccatga gctattatca
    10261 aaattacatc acaaaccttt attagacagt aagagagaaa atatatcttc agatgaactc
    10321 atatcattaa caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc
    10381 atacgccagt tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg
    10441 atagcctcag aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat
    10501 gagcaattaa caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg
    10561 gctattaact ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata
    10621 catagcgacc tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt
    10681 atagcacaca aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa
    10741 ttttccagcc cgttactaac aataaatggt gatgggcata cattagctct caccggagtt
    10801 aatttatgtg tagataaagc agttgtacat cacctgatca ctccacaaaa aatagaaaat
    10861 atatactgcc caggaaattc tgaagcagaa atacaataat ttcaaaatat tccgctattt
    10921 gctcccaatg caaaacatat attgcattgg gagcatattc atatttttgt tattattgta
    10981 tgaaatcgtc tcatgtacac ggtccttttt tcgccgaaaa ctgatattac tttaatcaaa
    11041 agttgacgtg ccagattcga agtaaattta taaaatatca aatcaatata cattttgcac
    11101 cttcgagata gtaatgactg ttactgtaac atgagttaca aacagcttca tacccagagc
    11161 tactgatgta tcaactcgat tcaacaatac ccgatattta ttccatgcct ccagcaacga
    11221 tctttcttcc tccgttgcga tttccagatc tacagcatcc tgcagtggcg caatatactc
    11281 actgaattcc tggatgtaga actgtgtggt gacggtcttc cagccattcg gctcctgttg
    11341 tatcgaagca taccaggcta tttcaatatc gctatgctgc ggcagcattt aaccccttgt
    11401 aattcatcgc cataattgat ttaattcaca aataaaacta taacatggtg aaattaatga
    11461 aaaaaaacac agatgatggg gctaaaattt acacaccact taccctaaag ctttatgact
    11521 ggtgggtttt gggagtatca aatcggcttg catggggatg tcctacaaag gaacaccttc
    11581 ttccacactt tctggaacat ttaggtaaca accatctgga tattggtgtt ggaactgggt
    11641 tttaccttac tcacgtacct gagagtagtc tgatatcttt aatggatttg aacgaagcta
    11701 gcctgaacgc ggcatctaca agggctgggg aatcaaaaat taaacataaa attagccatg
    11761 atgtttttga accttatccc gcggcgttac atggtcaatt tgattccatt tccatgtttt
    11821 accttcttca ctgcctgcct ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg
    11881 cgcaggcctt aactgacgat ggaactctat acggagccac aattcttggc gatggagttg
    11941 tgcacaatag cttcggtcaa aaactgatgc gcatttacaa tcagaaaggc atcttttcaa
    12001 acacaaaaga ttccgaagaa ggcttaacac atatactctc agagcatttc gagaatgtta
    12061 aaaccaaggt tcaaggtact gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa
    12121 ccgcagcacg ttcttgc
//
LOCUS       DAZGTR010000130        12116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGTR010000130 DAZGTR010000000
VERSION     DAZGTR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.132"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            293..553
                     /locus_tag="ACHLCY_003979"
     CDS             293..553
                     /locus_tag="ACHLCY_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0371145.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            744..884
                     /locus_tag="ACHLCY_003980"
     CDS             744..884
                     /locus_tag="ACHLCY_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0371146.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(1191..1490)
                     /gene="ihfA"
                     /locus_tag="ACHLCY_003981"
     CDS             complement(1191..1490)
                     /gene="ihfA"
                     /locus_tag="ACHLCY_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP0371147.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(1495..3882)
                     /gene="pheT"
                     /locus_tag="ACHLCY_003982"
     CDS             complement(1495..3882)
                     /gene="pheT"
                     /locus_tag="ACHLCY_003982"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP0371148.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATSDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(3897..4880)
                     /gene="pheS"
                     /locus_tag="ACHLCY_003983"
     CDS             complement(3897..4880)
                     /gene="pheS"
                     /locus_tag="ACHLCY_003983"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP0371149.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRSMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(5090..5214)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(5164..5208)
                     /gene="pheM"
                     /locus_tag="ACHLCY_003984"
     CDS             complement(5164..5208)
                     /gene="pheM"
                     /locus_tag="ACHLCY_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP0371150.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(5331..5687)
                     /gene="rplT"
                     /locus_tag="ACHLCY_003985"
     CDS             complement(5331..5687)
                     /gene="rplT"
                     /locus_tag="ACHLCY_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP0371151.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(5740..5937)
                     /gene="rpmI"
                     /locus_tag="ACHLCY_003986"
     CDS             complement(5740..5937)
                     /gene="rpmI"
                     /locus_tag="ACHLCY_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP0371152.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(6034..6576)
                     /gene="infC"
                     /locus_tag="ACHLCY_003987"
     CDS             complement(6034..6576)
                     /gene="infC"
                     /locus_tag="ACHLCY_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP0371153.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(6580..8508)
                     /gene="thrS"
                     /locus_tag="ACHLCY_003988"
     CDS             complement(6580..8508)
                     /gene="thrS"
                     /locus_tag="ACHLCY_003988"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP0371154.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            9032..10931
                     /gene="espL1"
                     /locus_tag="ACHLCY_003989"
                     /pseudo
     CDS             9032..10931
                     /gene="espL1"
                     /locus_tag="ACHLCY_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            11103..11210
                     /gene="yniD"
                     /locus_tag="ACHLCY_003990"
     CDS             11103..11210
                     /gene="yniD"
                     /locus_tag="ACHLCY_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP0371155.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(11263..12021)
                     /gene="ydiY"
                     /locus_tag="ACHLCY_003991"
     CDS             complement(11263..12021)
                     /gene="ydiY"
                     /locus_tag="ACHLCY_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP0371156.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGHQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         3168 a         3073 c         2722 g         3153 t
ORIGIN      
        1 gcagaagaaa agacagaaaa gttctattag cgcacaaatc aaaacatgag ggtgtactca
       61 aataatacaa tgctatagta aatctataag tgcataacta tttcctgatc ttttcccaaa
      121 atagatgaca ggataaaaaa cattccaatc ttttacaggc tttggataaa tcgtatcaaa
      181 gcctatttct tcaagagtta aatcaccgct tttcgctcga aacggactgg ctgttggagt
      241 atgagccaga acaacttaga atagcccctg cactacgtta agggaggtcg ctatgttccg
      301 ttgtccgctt tgtggcgcat ctgcccgtat ccgcaccagt cgtccggaaa atgattcaaa
      361 caccgtgcgg caaaagtatt accagtgtaa caatctggaa tgcggcgtat gcttctcaac
      421 actggaagct ttccataaat tcacatcaaa acacgcctcc ggcgttcact cttcagaaga
      481 tatcccgtgg catgatctgc cagcttcaca caggggaaac aatcagatga gcttgccttt
      541 acctcagaat taacaggcag aattgccgga gtaacaaaaa agcgatagat tacgcgcggg
      601 tgcctttcgg ctgatggtcg gagggaatac ccgaaggcca gatgtggaaa ggccccggaa
      661 aacatctctg tttaaccgag gccctaaccg cattaccttg acaagtgaaa ggttagcgcc
      721 tctccggaaa aggagcaagt gctatgtcgc aaaaatcgct tacgaccatc acgttctgcg
      781 tgacggcaat cctcatcatc tggatgttgc acggttcgct gtgtgaaata cggatgagct
      841 tctggggagc ggagtttgcg gcgttcttac agtgtaagca gtaaggaaac cgcgacgggg
      901 gagcaatccc ccgtcaatcg gttgccaggg taaggccgga taaggcaccc tatctcacag
      961 atatgaacaa caaacccgca gcgtaaaaac tgcgggtttt ctttttggtg ccctcaccga
     1021 cttgaaacag tgataagact ataagacaaa gcccgcaatg tagcgggctt actcccctca
     1081 ccctccactg aatgtggaca cacagtggaa cgaagcggca taagatatta taaatcaatc
     1141 agttattgaa agaaaaaagg ccgcagagcg gcctttttag ttagatcaga ttactcgtct
     1201 ttgggcgaag cgttttcgac ccggcttttt aacttctgcc cgggtctgaa ggtcaccacg
     1261 cgccgtgctg taatgggaat atcctcgccc gttttcgggt tacgtcccgg gcgttgattc
     1321 ttatcacgca gatcgaagtt accaaaacca gagagtttca cctgttcgcc gttttccaga
     1381 gcgcgacgga tctcttcgaa aaacagttca accagttctt tggcatcccg cttgctaagc
     1441 ccaagcttat caaacagata ttctgacatt tcagcttttg taagcgccat aggttcaatc
     1501 cctcaatgat gcctggaatc gctcttttaa tgcctctaca catttggcga cggtagcggc
     1561 aatctcctct tcttcgagtg tacggctggt atcttgcagg atcaggctta tggcgaggct
     1621 cttatacccc tccgcaacac ccttaccgcg gtacacgtca aataagttta cgccaactac
     1681 ctgatttacg ccaactttct tacattcgga taaaatatcc gctgcgggaa cgttttctgc
     1741 gaccaccacc gcgatgtcac gacggttcgc cgggaagcga gaaatctcgc gcgcctgagg
     1801 caccacgcgg tctgcgagct tgttccactc cagttcgaac accagagtgc gaccgttaag
     1861 atccagttta cgttccagtt caggatgaac aaccccaaca aaaccaatac gttcaccttt
     1921 cagataaatc gctgcggatt gccccggatg cagtgccgga ttcgcttctg cacggaactc
     1981 aacctcattc agtttaccgg tcaggtcgag aacggattca agatcgcctt tcaaatcata
     2041 gaaatcaacg gtctcttttg ccaggttcca gtgctcttcg taacggttac cgcaaatcac
     2101 accggctaac atcagatcct gacgaatgcc caacggtgcc tgagtatctg gtacgaaacg
     2161 cagaccgctt tcgaaaatgc gcacacggtt ctgctgacgg ttctggttgt acaccacggt
     2221 tgccagcagg ccggtccaca gagaaagacg cattgctgac atttcaacag agatcgggct
     2281 tggcagcagt aaggcttcaa cgcctggatg gatcatctgc tgcactttcg gatcaacgaa
     2341 gctgtaggtg atcacttcct gatagccttt gtcgttgagc agcgttttca cgcgcttgag
     2401 cgacaggtca gcttcacggt gagtacccat aatcaggctt gcctgtaccg gctcatccgg
     2461 gatgttgttg tagccgtaaa cacgcgcgac ttcttcgacc agatcttctt caatctccat
     2521 atcgaaacgc cagctcggcg caactgcctg ccactcgtct ttgccttcgg tcacttcgca
     2581 gccgagacga cgcagaatgt cagttacctg ctcatccgca atatgatggc cgatcaggcg
     2641 atccagtttg ctacgacgta aagtgatggt tgcacgcttc ggcagcgttg cttcgttggt
     2701 gatatcaatt accggaccag cctcaccacc gcagatgtcg atcagcagac gggtcgcacg
     2761 ttccatcgct ttgtgctgca gtgccggatc aacgccacgc tcataacggt gagacgcatc
     2821 agtatgcagg ccatgacgac gagcacgacc ggtgatagac agcgggctga agaaagcgca
     2881 ttccagcaag acgttttgcg tttcgtcatt cacgccagag tgttcgccac caaagatgcc
     2941 gcccatcgcc agcgccttgt tgtggtcggc gatgaccaga gtgtcagcat tcagcttcgc
     3001 ttcagtgcca tcaagcagta ccaacgtttc gccctctttc gccatccgca ccacaatgcc
     3061 gccttcaatg cgatctttat cgaaagcgtg catcggctgg cccagttcga gcagtacata
     3121 gttggtgacg tcaacaactg catcgataga acggatcccg caacgacgca gtttttcttt
     3181 catccacagc ggagttggcg ctttaacgtt aatgcctttt accacacggc caagataacg
     3241 cgggcaggct tccggcgctt cgactgtaat cggcagcgtg tcgtcgctgg tcgcaccaac
     3301 cggaacgatt tccggttcaa ccagcggcag ctgattcagc acggcaacgt cacgcgcaac
     3361 accaatgata cctaaacagt cggcacggtt tggcgtcacg ctgatttcga tggtgttgtc
     3421 atcaagtttc aggtattcac ggatgtcggt gccaatcggc gcatccgcag gcagctcgat
     3481 aatgccgcta tgatcgtcag aaattcccag ctcagaaaag gagcacagca tcccttcaga
     3541 cggttcacca cgcagtttcg ccgctttaat tttgaaatca cccggcagaa cagcaccaat
     3601 ggtagctacc gccacacgca ggccctgacg gcagtttggc gcaccgcaga cgatgtccag
     3661 caggcgatcg ccgccaacat tcacttttgt cacacgcagt ttgtcagcgt tcggatgctg
     3721 cgcacactca accacttcac caacgaccac gccgtggaag ctaccggcaa ccggttctac
     3781 accgtcaact tccaggcccg ccatagtgat ttgattcgcc agcgcatcgc tatcaatcgc
     3841 cgggttcacc cattcgcgta accacagttc actgaatttc ataatctatt cctgccttat
     3901 ttaaactgtt tgaggaaacg cagatcattt tcgaagaatg aacgcaggtc ggtgacgccg
     3961 taacgcaaca tagtcagacg ctccatcccc atcccgaagg cgaaaccaga gtaaacttcc
     4021 gggtcgatgc caacgttacg cagcacgttc ggatgcacca tcccgcaacc cagcacttcc
     4081 agccatttac cgtttttacc catgacgtca acttctgcag aaggctcggt gaacgggaag
     4141 taggaaggac ggaagcgaat ctgcaaatct tcctcaaaga agttacgcag gaaatcgtgc
     4201 agagtgcctt tcaggttggt aaagctgatg ttggtatcaa caatcagacc ttccatctga
     4261 tggaacatcg gcgtgtgagt ctggtcgtag tcgttacgat aaacgcggcc aggcgcaatg
     4321 atacgaatcg gtggctgctg ggctttcatg ctgcggatct gtacgccaga ggtctgggta
     4381 cgcagcaggc gggtagtgtc aaaccagaaa gtgtcgtggt cagcgcgcgc cgggtggtga
     4441 ccaggaatgt tcagagcatc gaagttatga taatcgtctt cgatttccgg cccggttgcc
     4501 acggtaaagc caagctcacc gaagaaactt tcgatacggt cgatagtacg ggtaaccgga
     4561 tgcagaccgc cgttttcaat gcgacgacct ggcagagaga catcaatcgt ttccgccgcc
     4621 agacgcgcat tcagtgcagc gctttccagt tccgctttac gcgcattcag cgcctgctga
     4681 acctgctctt tcgcttcgtt gataaccgca ccagctgccg gacgctcttc tggcggcagc
     4741 tcacgcaggg tcgtcatctg aagggttaag tgcccttttt tacccaaata ttcgacgcgc
     4801 acattatcta acgcggcaac atctgacgcc tggctaatgg ccgccttcgc actggcaacc
     4861 agttctgcga gatgtgacat ggttttcctc attgtgtcag tggtgacact ggttcgttgg
     4921 acttagagcc tatcccatca ggctatttta cttgccattt tggtcccggg cagtgctcga
     4981 aattctcacg tacttaaata cgctccggtt tctccgcgct ggccgtgtcc agactggctg
     5041 cgacaattac acctgatgag acaggctttt tatttttcaa aacgcgcata caaaaaaagc
     5101 ctccactggg aggctttcag gcgctgtttt ccgtttctct tctcacgcgc tagcctcctg
     5161 gattcaggtg ctaaagtaaa aaaagaagcg gaaaatagca gcattcattg cttgcgttac
     5221 cttttggtac tcttcaaaag acctttattg aaaaggctac ggcgataaaa gtcaatgttt
     5281 tgatggcgtt gaaacgaaaa gagggagact agctccctct ttcaactggc ttatgccaga
     5341 gctgctttcg ctttttcaac cagagcggtg aacgctactt tgtcgaatac tgcgatatca
     5401 gccaggatct tacggtcgat ttcaacagag gcttttttca ggccattgat gaatttgctg
     5461 taagaaatac cgttctgacg tgctgctgcg ttgatacgcg caatccacag ttgacggaac
     5521 tgacgcttac gttgacgacg gtcacggtaa gcatactgac cagctttgat aacagcctgg
     5581 aaggcaacgc ggtatacgcg agaacgcgca ccgtagtagc ctttagcttg tttcaaaatt
     5641 ttcttgtgac gtgcgcgtgc aataacacca cgttttacgc gagccatatg tgctctcctg
     5701 tatctatatt ctaattaaaa agttaaaaac gttaacggct tatgcgtacg gcaggcacgc
     5761 gattaccagg cccagatcgc ctttggaaac catggctttc ggacgcaggt gacgtttacg
     5821 tttggtcgct tttttggtca gaatgtgacg caggttagcg tgcttgtgct taaaaccacc
     5881 tttaccggtt tttttgaagc gcttagcagc accgcgtacg gtcttaattt ttggcatttt
     5941 aataacttcc acttcgcatt gttaataaac gaaacaaagg cgaacaaagc ctgtgaagcc
     6001 cgaaggctcc acagacagtg ctacttgaag gccttactgt ttcttcttag gagcgagcac
     6061 catgatcatc tggcggcctt cgatcttcgt tgggaaggat tcgaccactg ccagttcttg
     6121 caaatcgtct ttcacgcgat taagcacttc cataccgatt tgctggtgcg ccatctcacg
     6181 accgcggaaa cgcagcgtga ttttggcttt atcaccctct tcgagaaagc gaatcaggct
     6241 gcggagtttt acctgatagt cgccttcatc tgtaccagga cggaatttaa tttccttaac
     6301 ctggataact ttttgctttt tcttctgttc cttagaagac ttgctctttt catagaggaa
     6361 tttgccgtaa tccattatac gacaaaccgg cggctcggcg ttagggctga tctcgactaa
     6421 gtctactccg gcttcttctg ctttctccag agcttctctc agactcacaa taccaagctg
     6481 ctcgccttcc agacctgtta agcgaacttc ctgggcgcga atttcgccat tgatacggtt
     6541 agggcgcgcc gtttgaactc gttttccgcc tttaatacct tattcctcca attgtttaag
     6601 actgcggctg cgaatctctt gttgcagctt ctcgatcact tcatttacgt ccatgcttcc
     6661 caggtcttta ccacggcggg tgcgaacggc aactttgcct gattccacct ctttatcacc
     6721 acagaccagc atatatggga cgcgacgcaa agtgtgctcg cggattttaa agccaatctt
     6781 ctcatttctc aagtctgctt taacacgaat gcccgcattt gatagttttt gcgtcaattc
     6841 gttaacgtat tcagactgtg aatcggtaat attcatgata acaacctgaa ctggcgcaag
     6901 ccaggtcggg aagaaaccag cgaattcttc ggtcaggata ccgatgaaac gttccatcga
     6961 tcccagaatt gcgcggtgaa tcattaccgg tactttacgt tcattgtctt cgcctacata
     7021 agaagcgctc agacgagaag gcaaagagaa gtccagctgt actgtaccgc actgccatgc
     7081 acgatcgagg cagtcataca gggtaaattc aattttcgga ccgtagaaag cgccttcacc
     7141 cagttgatat tcaaacggga tgttgttttc ttccagcgca accgccaggt ccgcctcagc
     7201 acgatcccac atttcatcac tgccaatacg tttttcagga cgagtggaga gtttgacgac
     7261 gatcttctcg aagccaaaag tgctgtacat atcatagact aaacggatac atccgttaac
     7321 ttcatcgcga atttgttctt cagtacagaa gatatgcgcg tcatcctggg taaatccacg
     7381 cacgcgcatc aggccatgca gcgaacctga cggctcatta cggtggcagc taccaaactc
     7441 ggccatacgc agcggcagat cgcgataaga cttcagcccc tggttgaaaa tttgtacgtg
     7501 acccgggcag ttcatcggct taatgcagta ttcacggttc tcagaagacg tggtgaacat
     7561 tgcatctttg tagttgtccc agtgaccggt tttttcccac aggacacggt ccatcatgaa
     7621 cggaccttta acttcctgat actggtactc tttcagttta gaacgaacaa acacttctag
     7681 ttcacggaag atggtccagc cgtcgttgtg ccagaatacc atacccggcg cttcttcctg
     7741 catatggtac aggtcgagct gtttaccgat tttacggtgg tcgcgtttcg cggcttcttc
     7801 caggcgctgc aggtaagcgt taagcgcttt tttgtctgcc cacgccgtac cgtaaatacg
     7861 ttgcaacatt ttgttgttgc tgtcgccacg ccagtaagcc cctgccgttt tcattagttt
     7921 gaaatgatgg cagaaacgca tgttcggtac gtgcggaccg cggcacatat cgacatattc
     7981 ttcatggaag tacagacctg gcttgtcatc atgggcgatg ttttcgtcaa gaatggagac
     8041 tttgtagctc tccccacggt tggcgaaagt ttcacgcgct tcgtgccagc tgactttctt
     8101 cttaatgacg tcgtagtttt tctcagcaag ctcatgcatc cgcttctcga gtgcttcgac
     8161 atcttcctgg gttaacgtgc ggtcaagatc aacgtcgtaa taaaaaccgt tgtcaataac
     8221 cgggccgatt gccattttgg tatgcggcca aagttgttta atcgcgtgcc ctaacaggtg
     8281 cgcacaggag tgacgaatga tctccagacc gtcttcgtct ttggcggtaa tgatggacag
     8341 ttgtgcgtcg ttttcaatca gatcgcaagc atcaaccagt tcgccattaa cgcgccctgc
     8401 gatacaggct ttcgccagac ctggaccaat gtccagcgca acatccatgg ggcttacagc
     8461 gtggtcgtaa tggcgttggc tgccatcagg aagagttata acaggcattt tatatcctta
     8521 tttgcagtgg tgacccacac gaaagatcac atacaaagaa aaatttgttt attaacagtt
     8581 cattgcgaaa ccatctagcc aacaaatgct aaattggttc gcaatcaggt acacaaatga
     8641 aagacattaa cctctcacct tccacccggt tgagcgatgt taacactaaa aaaagggaga
     8701 ttgtaccttt ccgtttcaca ttgattcgat tcgcaattcg tttgtagtga gtacaccagc
     8761 aaaacgctta aagaaccact gataacatca cggtgcgtct gaaaggttta ctgcgagcgg
     8821 atttacagca gcataaactg aatccagcaa agttcgttgc gtgatttggc ggtagtattt
     8881 taaacgctca tgagattaat gacgaagtgg tcatatcaca atgataaaag tgacacaatt
     8941 cttataacaa tttttcgtgc acatttcgtt ctggcgataa taattaatca tcatcctcaa
     9001 aacctccaga tatatataag gcgaatagat tatgagtcaa aacgatatca ttatcagaac
     9061 tcattataag tctcctcata gattgcacat cgatagcgac ataccaacac cttcatcaga
     9121 gcctattaat aaatttgcgc gccagctcat caccctactt gatacctctg acttaagttc
     9181 gatgctgtca tactgtgtta ctcaggaatt taccgcaaac tgtcgaaaaa tatcacaaaa
     9241 ttgttattcc actgcccttt ttaccattaa ccttgccact tcacccatcc atgcagaaaa
     9301 tatactcatt acattacact ataaaaaaga tatcatttcc ttattactgg aaaccacgcc
     9361 tattaaagct aaccatttgc gaagcatact ggattatatt gaacaggaac agttaactgc
     9421 tgaagatcgt aaccattgta tgaaactgtc taaaaaaatc catagagaaa aaactataca
     9481 cccaacagta aatctcaatg gtagtgcatg ttttttgcaa tctccttctg acgctatttt
     9541 ttgtcgccat ctgtcattgc aatacgccct tgattcattg agaaatggaa aaggcaaagt
     9601 caacctgatt aaacattact cctccgttga atccatacag cagcatgtcc ccttagtccg
     9661 ggacgcggag ttcagagcat tacttcgcca tcctcctgca gggagtcgcg ttatcgcgag
     9721 taaggatttt ggcttcgctt tagatttttt cttttgtcga atgatggcaa acaatatcag
     9781 tcatatgtcc gcgattttat atatagacaa tcatactttg tcagtaaggc tacgaataaa
     9841 gcagtcagtg tatggacaat taaactatgt tgtgtccgtt tacgacccga acgataccaa
     9901 cgttgccgtc agagacaccc acaggacagc acggggcttt ctttcgcttg ataagttcat
     9961 cagttcaggt cccgatgctc agacctgggc tgataggtat gttcgcaact gtgcaattgc
    10021 tattctgccc ctattacctg tgggagttcc aggggctatt ttcgcgggta ttgcatcacg
    10081 aatgccattt gcccctatac atccatcggc aatgttgtta ataatggcta caggccagac
    10141 tcaacagctt attacattat tcaaacagtt gcccatactc cctgaaaaag aaatcattga
    10201 aataataact gcgcagaata gcgttggtac acctgcttta tttttggcta tgatgaacgg
    10261 acatactgac aacgtgaaaa tatttatgca agaaattcag tcactggtag ataatcacat
    10321 cattcatgaa gataacctgg ttaaattact gcaaactaaa agtgctaacg aaatacctgg
    10381 actctatatc tccatgttgt atggattcga tgaaataatc gatatctttc tgaatgcatt
    10441 aaccactcca atagcacaag aacttttaaa caaaaaaatg gtgatggata ttttagccat
    10501 gaaaacacgt gatggtgaac cagggttatt tgctgcaatg gaaaataatc accctttgtg
    10561 tgtcacacgg ttcctctcta aagtttatgg aatcgccgtt aaatataaac tcagcaaaat
    10621 caacatcatg gacttattaa aaggcgcgac agcacaggga acccctgcat tatacatcgc
    10681 catgagcaag ggtaatgaag acgtcgtgtt atcttatata tcgacgctgg gtgcttttgc
    10741 aaaaaaaaca ttcttttagt caacatcagt tatttacact attggccgct aaaaatcatg
    10801 acaacatgtc agctgttcat atagccattc atcataagca ttataaaact gtagaaacat
    10861 attatgctgc tataaatgtc atcagccaaa gcctgagttt tagtgctgat gaaataaaga
    10921 cgtatttata accagctatt tacgatatat atcgatacat aattttattt ttttcataaa
    10981 acgcagaaac agtgaccagg tgtacaacgc cacccaacgg ctgtaataac aaaaaatccc
    11041 ttgatgcccg tcccttttgt tacactccgt tatcacgcac aagagatatg caggacactg
    11101 gtatgccgac taaacgcttt gataaaaaac actggaagat ggtggtggtg ctactggcaa
    11161 tctgtggcgc tatgttgttg ctacgttggg cagcaatgat ttggggctga gcagtgacaa
    11221 acgaagacag ccagaccgca taacacggtc cggcagataa ttttacatgc tgtagcccag
    11281 cgataaggtt gtccgacggt cggtatgttc cggtgcagac tctggaggtt cagagttcca
    11341 tgtgacgttg taagccacct tcagtccaaa atgttcatta atagcaacat ttaatgcgct
    11401 ttcggagttc agcgttgtgt cttccgcgcc aaagaccgaa acaccctgcg taaatttagc
    11461 gttgtcagtc aactgccatg cataagcccc ggaggcatag cccagcggct gggtttcact
    11521 ggcattgtcg gtatatttgt cgtaacgcac acctggaccg aattcaaagc ggaaactgtg
    11581 taccgggcca ttcaaaaact gatgaccata acccgcggtc aacacatcgc gctctcgata
    11641 gccgttataa cggtctgtca gccagcttgc ctgaccaaat aaatagtcat aatcagttaa
    11701 attaaaacgg ctacgcccgc ccgccgcata tttttctgaa gaacgctcat cattagaaga
    11761 agtattactg gcgttccccc acagcgacca ggcagtggtt tgtccatacc aggtcatggt
    11821 ggtgtcagcc gtaagtgagg agcttttcgt attgcctgat tgtgcaagat atcctgcgtt
    11881 cagattacct tcgaaaggtt ttttagcgct ggcaggatcg tccatgacag taaaaacgga
    11941 atcatcggca gctgcattca gtgacgcaaa catgcccccc gccaacataa cgatggcagg
    12001 aactgtcttc aaaagcttca tttatcaaga gtccgtacaa caaaaaaaga gaccatcgcg
    12061 gtcccggaaa ctttcttaag gatcaaagat tagcgtccct ggaaaggtaa cgaatt
//
LOCUS       DAZGTR010000131        12054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGTR010000131 DAZGTR010000000
VERSION     DAZGTR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.133"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            27..629
                     /gene="yebB"
                     /locus_tag="ACHLCY_003992"
     CDS             27..629
                     /gene="yebB"
                     /locus_tag="ACHLCY_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP0371157.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(631..1152)
                     /gene="ruvC"
                     /locus_tag="ACHLCY_003993"
     CDS             complement(631..1152)
                     /gene="ruvC"
                     /locus_tag="ACHLCY_003993"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP0371158.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1187..1927)
                     /gene="yebC"
                     /locus_tag="ACHLCY_003994"
     CDS             complement(1187..1927)
                     /gene="yebC"
                     /locus_tag="ACHLCY_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0371159.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1956..2408)
                     /gene="nudB"
                     /locus_tag="ACHLCY_003995"
     CDS             complement(1956..2408)
                     /gene="nudB"
                     /locus_tag="ACHLCY_003995"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP0371160.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2526..4298)
                     /gene="aspS"
                     /locus_tag="ACHLCY_003996"
     CDS             complement(2526..4298)
                     /gene="aspS"
                     /locus_tag="ACHLCY_003996"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP0371161.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4608..5174
                     /gene="yecD"
                     /locus_tag="ACHLCY_003997"
     CDS             4608..5174
                     /gene="yecD"
                     /locus_tag="ACHLCY_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0371162.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5171..5989
                     /gene="yecE"
                     /locus_tag="ACHLCY_003998"
     CDS             5171..5989
                     /gene="yecE"
                     /locus_tag="ACHLCY_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP0371163.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6042..6437
                     /gene="yecN"
                     /locus_tag="ACHLCY_003999"
     CDS             6042..6437
                     /gene="yecN"
                     /locus_tag="ACHLCY_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP0371164.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6478..7221
                     /gene="cmoA"
                     /locus_tag="ACHLCY_004000"
     CDS             6478..7221
                     /gene="cmoA"
                     /locus_tag="ACHLCY_004000"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP0371165.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7218..8189
                     /gene="cmoB"
                     /locus_tag="ACHLCY_004001"
     CDS             7218..8189
                     /gene="cmoB"
                     /locus_tag="ACHLCY_004001"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP0371166.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FETVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8354..10783)
                     /gene="torZ"
                     /locus_tag="ACHLCY_004002"
     CDS             complement(8354..10783)
                     /gene="torZ"
                     /locus_tag="ACHLCY_004002"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP0371167.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMLHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10808..11908)
                     /gene="torY"
                     /locus_tag="ACHLCY_004003"
     CDS             complement(10808..11908)
                     /gene="torY"
                     /locus_tag="ACHLCY_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP0371168.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2767 a         3209 c         2916 g         3162 t
ORIGIN      
        1 catatctatt tcaatggaat gacaacgtga atattaatta ccctgctgaa tatgaaattg
       61 gtgatatcgt ctttacatgt ataggtgctg ccttatttgg tcaaatatca gctgcatcaa
      121 actgctggag taatcacgtc gggatcatta tcggtcataa cggtgaagac tttctggttg
      181 cagaaagccg tgttcccctc tcaaccatca ctacgctatc ccgttttatt aaacgctctg
      241 ctaatcaacg ctatgctata aagcgattag acgccgggct gacagaacaa cagaaacaac
      301 gaattgttga acaggttccc tcccggctac gcaaacttta ccacacgggt tttaaatacg
      361 aatcttcccg ccagttctgt tcaaaatttg tctttgatat ttataaagag gcgctatgta
      421 ttccggtggg tgaaatagag acgtttggac aactgttaaa tagcaatccg aatgcaaaac
      481 tcactttctg gaaattctgg ttcttaggtt ctattccgtg ggagcgtaaa accgtcacgc
      541 cagccagttt gtggcatcat ccgggtttgg tgttgattca cgcggaggga gttgaaacgc
      601 ctcagcctga actgaccgag gcggtataac ttaacgcagt cgccctctcg ccaggttcag
      661 ccgcgattcg ctcatctgca ttgcattctg gctgacgtga caatgggtaa tcgcgatagc
      721 taacgcatca gcggcatccg cttgtggatt agcgggcagt ttcagcaagg tgcggaccat
      781 atgctgcacc tggcttttct cggcgctacc aatacctacc accgtttgct ttacctgacg
      841 tgccgcgtat tcaaataccg gcaattcctg attcaccgcc gccacaatcg ccacgccgcg
      901 cgcctggccc agtttcaggg ctgagtcagc gttcttcgcc ataaagactt gttcaatggc
      961 gaaataatca ggctggaact gggtgatgat ttccgtcacg cccgcataga tgagcttcag
     1021 acgagacggt aaatcatcca ctttggtgcg tatgcatcca ctacccaggt aggacagttg
     1081 cctgcctacc tggcggatga cgccatagcc ggtcacgcgc gaacccggat caatgccgag
     1141 aataatagcc atcacgcgtc tccgttttgc tgtttagcag gcctcatcag agagtcgctg
     1201 caacctcatc agagatttca ccgttatggt aaacttcctg cacgtcgtcg caatcttcca
     1261 gcatatcgat cagacgcatc agtttcggtg cggtttctgc atccatatca gctttggtgg
     1321 acgggatcat ggaaacttcc gcgctgtctg ctttcagacc tgccgcttcc agagcgtcgc
     1381 gcactttacc catttcttcc catgcggtgt agacatcaat cgcgccgtca tcataggtca
     1441 caacgtcttc agcacctgct tccaaagctg cttccatgat ggtgtcttca tcgcctttct
     1501 cgaaggagat cacgcctttt ttgctgaaca gataggctac ggaaccatca gtaccgaggt
     1561 taccgccaca tttgctgaat gcatgacgca cttcagcaac ggtacggttg cggttgtcag
     1621 acagacattc aatcatgatt gccgtgccgc caggaccgta accttcgtag atgatggttt
     1681 ccatgtttgc atcatcatca ccgcccacac cacgtgcaat tgcgcggttc agtgtgtcgc
     1741 gggtcatgtt gttagacagt gctttatcaa ttgctgcacg cagacgcggg ttagcgtccg
     1801 gatcgccacc gcccagctta gccgcggtta ccagctcacg aatgatttta gtgaagattt
     1861 taccgcgctt tgcatcctgc gcagctttac gatgtctggt gttggcccat ttactatgac
     1921 ctgccataaa aatatctcca gatagccctg cctgttcagg cagcgttaat gacaaactgt
     1981 tcaatcgcct gccggttgct ccaggactta gtgagcgccg ccgcagcagg cgcatcaagc
     2041 cacttgtaag ccagatgttc agtgaaaacg atctgccgct cgtgcggaag cgcaagacag
     2101 aaccatgatt ccgtattacg cgtcacgccc ggcgcatagc gatgacgtaa atgtgaaaaa
     2161 atttcaaact ctaccgtgcg ctgacagtca attaaggtca gttgttcagc gacaacatca
     2221 atggtgacct cttcctttac ttcgcgcatg gcagcttgcg gcgcggtttc accctcttcc
     2281 acgctgccgg ttaccgactg ccagaaatcg ggatcgtcac gccgctgcaa catcagcacc
     2341 cgtttcgtat cttgtgcata gatgaccact aagatcgaaa cgggacgctt atacacttta
     2401 tccttcacgc tgcctcttcg ttgactgcct tcgctcatcc cattcacata gttatctatg
     2461 ctcatgggag ttcactcagt tgccgccttg atgcaacgcg aatgatttcg tgtatttgag
     2521 tcatatcagt tattctcagc cttcttcaca acctgaatgc tcagctcagc cagtgcagtc
     2581 gggttagcaa agctcggtgc ttcagtcatc agacacgctg ccgccgtggt tttcgggaag
     2641 gcgataacgt cacggatatt gtcggtgcca gtcagcagca tggtcagacg gtcaagaccg
     2701 aatgccagac ctgcgtgcgg cggagtaccg tatttcaggg cgtcgagcag gaagccgaat
     2761 ttctcgcgct gttcctcttc gttgataccc agaataccaa acaccgtctg ctgcatatca
     2821 ccattatgga tacgtactga accaccgccc acttcgtaac cattgatgac catatcgtaa
     2881 gcgttcgcca ccgcattttc cggtgcagct ttcagttctg cagcagtcat gtctttcggt
     2941 gaggtgaacg gatggtgcat tgccgtcagg ccgccttcac cgtcgtcttc aaacatcggg
     3001 aagtcgataa cccacagcgg tgcccatttg ctttcgtcgg tcagaccaag gtctttaccc
     3061 actttcaggc gcagtgcgcc catcgcgtcg gcaacaattt tcttgttgtc ggcaccgaag
     3121 aaaatcatat cgccatcttg cgcggcagta cgatccagga tggcttcgat gatttctgca
     3181 ttaaggaact tcgctaccgg gctgttgata ccttccagac ctttcgcgcg ttcgttaact
     3241 ttgatgtaag ccagaccttt cgcgccgtag attttaacga agttaccgta ttcgtcgatc
     3301 tgcttacggg tcagcgatgc gccacccgga acacgtagag cggcaacacg gcctttcgga
     3361 tcgttcgccg gccccgcaaa tactgcaaac tcaacagatt tcagcagatc ggcaacgtcg
     3421 gtcagttcca tcgggttacg cagatccggt ttatcagaac cataacggcg ttctgcttcc
     3481 gcaaaggtca ttaccgggaa atcgcccaga tccacgccct tcacttccag ccacaaatga
     3541 cgcaccagcg cttccatcac ttcacgcact tgcggcgcgg tcatgaaaga agtttccaca
     3601 tcgatctgag taaattcagg ctgacgatca gcgcgcaggt cttcgtcacg gaagcattta
     3661 acgatctgat agtaacggtc aaaaccggac atcatcagca gctgtttgaa caactgtggg
     3721 gattgcggca gtgcgtagaa tttacctttg tgcacacgag aaggcaccag gtagtcacgc
     3781 gcgccttccg gcgtggcttt ggtcagcatc ggagtttcga tgtcgaggaa gccgtggtca
     3841 tccataaaac ggcgcaccag gctggtgatt ttagcgcggg ttttcaggcg ctgagccatt
     3901 tccggacgac gcaggtcgag gtagcggtat ttcagacgcg cttcttcggt gttgacgtgg
     3961 ttagagtcaa gcggcagaac atctgcgcgg ttgatgatag tcagcgagga cgccagcact
     4021 tcgatttcgc cggtcgccat atcgcggtta atattttttt cgtcacgcgc acgtacggtg
     4081 cccgtgacct gaatgcagaa ctcattacgc agttcagagg ccagctttaa cgcgtccgca
     4141 cgatccggat cgaaaaatac ctgcacgata ccttcgcggt cgcgcatatc gatgaagatc
     4201 aggctaccaa gatcacgacg acggttgacc caaccacaca gagtcacctg ctgccccacg
     4261 tgggacaaac ggagctgtcc acaatattct gtacgcatga gatatccctt aacttagctg
     4321 ccggcggatg ccccctgctg cgcaggtgac caagtcgcag cgttagctgt atgtcacaac
     4381 tgaatgaaaa aaggcggcta ttatactgga aattctgccg caccgtaaga gcctggcccg
     4441 cgctggaacg cctcgttacc actttatatc gggcctgaaa tcagactcta cgccagtttg
     4501 ctataaaggt gttgcccgaa ctcataaaaa ttaacaaaat ttgtcgttcc gccatcggct
     4561 aatcgcatta aggtgtgagg cacgattttg ttttttcagg agtcattatg cttgaactta
     4621 atgctaaaac cactgcgctg gtggtgatcg atttacaaga aggcatctta ccttttgccg
     4681 gtggtccaca tactgccgat gaggtagtta atcgcgccgg gaagctggcg gcgaaatttc
     4741 gcgccagcgg tcagcccgtg tttctggtgc gcgttggctg gtctgccgat tacgccgaag
     4801 cattaaaaca gccggttgat gccccctccc ccgctaaagt gttgcccgaa aactggtggc
     4861 aacatccagc tgcattaggt gcaactgaca gtgatatcga aatcatcaaa cgtcagtggg
     4921 gtgcgtttta cggtacggat ctggagttgc aactgcgccg ccgaggtatc gatacaatag
     4981 tgttatgtgg gatctcgacc aatatcggtg ttgaatccac cgcccgcaat gcctgggaac
     5041 ttggctttaa tctggtgatt gccgaagacg cctgtagtgc cgctagcgcc gagcagcaca
     5101 ataacagcat caaccatatc tacccacgca tcgcccgtgt gcgtagcgtg gaagagatcc
     5161 ttcacgcgtt atgatttaca tcggtctacc gcaatggtcg catcctaaat gggtgcggtt
     5221 ggggatcacc agccttgaag agtatgcccg ccactttaac tgcgtggagg gcaacaccac
     5281 gctttacgcc ctgccgaaac ccgaggttgt cctgcgctgg cgtgaacaga ccacagatga
     5341 cttccgcttc tgttttaagt ttccggcgac catttcgcat caggcagcat tacgacattg
     5401 cgatgattta gtgactgaat ttttgacccg catgtcaccg ttggctccgc gcattgggca
     5461 atactggctg caactgcctg ccacattcgg cccacgggag ctgcctgcgc tttggcattt
     5521 tctcgattct ctccctggcg aatttaatta tggtgtggaa gtccgccatc cacagttttt
     5581 cgccaaaggg gaagaggaac aaacgcttaa tcgcggttta catcagcgcg gcgttaatcg
     5641 ggtgatttta gacagccgcc cggttcatgc agcatgtcca cacagtgaag ctattcgcga
     5701 cgctcaacga aaaaaaccta aagttccggt acatgctgta ctgacggcga caaatccgct
     5761 gatccgtttt atcggtagtg atgatatgac gcaaaaccgg gaattatttc aggtctggtt
     5821 acagaaatta gcgcagtggc atcagaccac tacgccttat ctttttttac atacgccaga
     5881 cattgcccag gccccggaac tggtacatac cctgtgggaa gacttacgta aaacgcttcc
     5941 agagatcgga gcagttccgg ctattccaca gcaatcttct cttttctgaa tttgccacct
     6001 atcatagaca ggtgccatcg gccattttaa agggagtttg tatggtaagc gcgctatatg
     6061 ccgttttaag tgcgttgtta ttaatgaagt tctcttttga tgtcgttcgc ttgcgtttgc
     6121 agtaccgcgt tgcctatggt gacggtggtt ttagcgaact gcaaagcgct attcgcattc
     6181 atggtaacgc ggtggaatat attcccatcg cgattgtgct gatgctgttt atggaaatga
     6241 atggcgcaga aacctggatg gtgcatattt gcggcatcgt tttgcttgct ggtcgtctga
     6301 tgcattatta cggttttcat caccgtctgt ttcgctggcg acgttccggc atgagcgcca
     6361 cctggtgtgc gctgttgctg atggtgctgg cgaatctttg gtatatgccc tgggagttgg
     6421 ttttctccct gcgttagcgc acaatacgcc actttctttt tcccggattt ttacgttatg
     6481 tctcaccgcg acacgctatt ttctgcccct atcgccagac tgggcgactg gacctttgat
     6541 gaacgggtag ctgaagtctt cccggatatg atccagcgtt ccgttcccgg ctattccaat
     6601 attatttcca tgattggtat gttagccgag cgcttcgttc aacctggtac gcaggtttac
     6661 gatctgggtt gttctctggg cgcggcgacg ctctcggtgc gtcgcaacat tcatcatgat
     6721 aattgcaaaa ttattgccat cgacaactcc ccggcgatga ttgaacgctg ccgtcgtcat
     6781 attgacgcct ataaagcccc tacgccagta gacgtcattg aaggtgatat tcgcgatatc
     6841 gccattgaaa acgcatcgat ggtggtgctg aattttaccc tgcaattcct ggaaccttcc
     6901 gagcgccagg cattactgga taaaatttat caaggactga acccgggcgg cgcgctggtg
     6961 ctttcggaaa aattcagttt cgaagatgcc aaagttggtg aactgttgtt caacatgcac
     7021 cacgacttta aacgtgccaa cggttacagc gaactggaga tcagccagaa gcgcagcatg
     7081 ctggaaaacg tgatgctgac cgattccgtg gaaacccata aagcacgcct gcataaagcc
     7141 ggttttgagc atagcgagct gtggttccag tgctttaact ttggttcact ggtggcatta
     7201 aaagcagagg acgctgcatg atcgactttg gtaactttta ttctctgatt gccaaaaatc
     7261 atctttcaca ctggctcgaa acgctgcccg cgcagattgc taactggcag cgcgagcagc
     7321 agcacgggct gtttaagcag tggtccaacg cggtggaatt tctgcctgaa attaaaccgt
     7381 atcgtctgga tttattgcat agcgtaaccg ccgaaagcga agagccactg agcaccgggc
     7441 aaattaaacg cattgaaacg cttatgcgga acctgatgcc atggcgcaaa gggccgttct
     7501 cactgtatgg cgtcaacatc gataccgaat ggcgttccga ctggaaatgg gatcgtgttc
     7561 tgccccatct ttctgattta accggacgca ccattctgga tgtcggctgc ggcagcggtt
     7621 atcacatgtg gcgcatgatt ggcgcaggcg cgcacctcgc ggtggggatc gaccctacgc
     7681 aactgtttct gtgccagttt gaaacggtgc gtaaactact gggtaacgat cagcgcgcac
     7741 atttgttacc gttaggtatt gaacagcttc cggcactgaa agcctttgat accgtctttt
     7801 cgatgggcgt gctttaccat cgccgttcgc cgctggagca tctctggcag ttgaaagatc
     7861 aactggtgaa tgagggtgaa ctggtgctgg aaacgctggt tattgatggc gatgagaaca
     7921 cggtgctggt accaggcgat cgttacgcgc aaatgcgtaa tgtctatttc atcccttccg
     7981 cgctggcgct gaaaaactgg ctgaagaagt gtggttttgt agatatccgc gttgtggatg
     8041 tgtgcgttac cactactgaa gagcagcgac gcactgaatg gatggttacc gaatctctgt
     8101 ccgatttcct cgacccacat gatccgagta agacagttga aggttatcct gcgcctaaac
     8161 gcgcggtgct gattgcgcgc aagccgtaaa ggtctggcat tactgccgga tgcggcttga
     8221 atgccttatc cggcctacaa acccttgcta attcaatcta ttgcagggac tatgtaggcc
     8281 tgataagcat agcgcatcag gcagttttac gtttgcataa cctcagcgcc cgtttccggg
     8341 cgctattcac ttcttacgcc tgaacagcag gcttatcaaa tgccgttaac ttcggtgcgt
     8401 tacccgtata tttttcgata tacaccagcg cagagttacc ggcacaggca tttgccagct
     8461 gcgagctggg gatatccgcc gttaacacgt tcgcactgcc gtttttacac aagccgtttt
     8521 ccagatctgg ccatgcacct tcatgcacgc ataccacgcc ttttttgatc ccgtcagtca
     8581 ccaccgcgcc tgtcaaaatc tgaccacgtt tgttccagac acgcaccaga tcgccattcg
     8641 caataccaaa tcgagcagca tcttcggtgt gaatagtgat cggttcacga tcggcaaccg
     8701 catatttttt acgcagttcc gcatagttaa gctgactatg taaacggtgt gccggatgtg
     8761 cggtcagaag ctgcaactgc ttctcgtcgg cggtaccctt ccactcatca ggcgcaagcc
     8821 aggttggatg tgccgggcaa tccttatagc caaatttttc cagcgttttg gagtaaatct
     8881 caattttgcc gcttggcgta cccagcgcat ttttcaccgg atcggcgcgg aaatcagcat
     8941 aacgaacgta ctgttcgttc ttctcgctgc ggcgcatttc gatcagttta ttttgctgcc
     9001 agaaggcatt aaacatcggc atagtcacgc gttgcgcacg agcacctttc tgagcggcat
     9061 catagaaaaa tttcagccac gccatttcat ctttaccttc ggtatagatc tcttttccgc
     9121 caggtttcag taattcagca agatcggcaa atacgtcaaa atcgttacgc gcttcaaatt
     9181 gcggagcaac agcctgcttc atcggcacaa tatgctggtt gctgtaatca ccggtcatcg
     9241 tcaagtcatt gcgctcaaac gatgtggtga tcggtaatac gatatctgca tgtttagcag
     9301 ctgcggtcca gtagcattca gaaacgacga tcatctccgg tttctgccat gctttgatca
     9361 gacgattagt atcctggtga tgggtaaagt taccgccacc cgcccaccag atcattttga
     9421 tattcggata cgtttgttct ttaccgttat gctgatattt gccaccagga ttttccagcg
     9481 catcgacgat acgggcaaca ggaatcgccg tcattccgcc atcatcagcg gcctcgctgg
     9541 cctgtccggc aatcgccgca gacatttccg gcagcacgcc gccaacacgc gtcggattgc
     9601 caccgttgga gtaatgataa gagaaaccaa aaccgccgcc cggtgtacca atctgcccta
     9661 acatcgccgc cagcgtcacc agcatccagt gtttctgttc accatattgc tggcgctgaa
     9721 ttccccagcc agccatcagc attgtgcgat tcgccgccat cagttcagcg agtttgacta
     9781 tctgcgcttc cggtacgcca gtaatttccg ccgcccaggc cgcgctcttc ggagtattat
     9841 cgctcttacc tgtcagatac tcttcaaact gcggataccc ggtggtgtat ttttcaagga
     9901 acactttatc gtgtttacct tgtgtcatca gggtatgcgc aatccctaac atcagtgcca
     9961 cgtcggtgcc catattcggc gcgatccagg tggcattatc accaaagaac tcgatggttt
    10021 cggagcggat aggatcaatg gcaatcactg gtttgccaga ttttttcagc tgatggaagt
    10081 attccagccc ttgctcatcg gtactgctcc aggcaatttt taaggtattc agcgggttca
    10141 tcccccacag caccacagcc tggctgtttt ccagaatcag cggccaggag gtctgctgtt
    10201 catacacctc tacagaaccg accacatgca gcatgatcac ctgtgctgcc ccggttgaat
    10261 aatcgccgct atgcccggaa taaccgcccg ccaggttcat ataacgttgc agtaaggttt
    10321 gcgctttatg caacacgcca gaagagcgcc agccgtatga tccggcaaaa atagcagatg
    10381 gtccgttagc tttgcgaatg cgatcgtgtt gctcatgaat cagttttaat gcttgctccc
    10441 agctcacctg tacataagta tcttcgccac gacctttcgc cggttgcagt ggattatcga
    10501 gatagctttt tcttaccatc ggatgctgaa tacgtgccgt ggtgtgtacc tgatccgccg
    10561 ccgtagactg taaggagttc ggtatggttt tcgccagcgc gcctgttgaa gaaacaatct
    10621 tgccgtcctt cacttctacg ttcatcgctc cccaacgtcc cgcggtgagg attttaccgc
    10681 ccttctcttc tgcccatgcg gggagcggtg ctgccgatgt caccaccagc gctccagcgg
    10741 caataccgct gtgtttaata aattcacgtc ttgttaatgt cataacttcc tccctgatca
    10801 acgaggatca ctgtttctcg gtaatatctt tggcgttgta ctggaaatac cgcgttaaaa
    10861 tgtccagttc gttttcgctc atgctggttc gcgcacccat tcctttggca atggacggcc
    10921 atgcattgac ggtgtaatgg tcagcggcaa taggcgcatg gcaaccagcg caataggtat
    10981 cgtcaagttt ttcagcgtat tgccatagcg gtttacggtc cgctaatgcg ggatcggtaa
    11041 gcgcaccctg taaagacgcc tgacgccatt gattgccgta ttcgtcagcc tgccattccc
    11101 cgtttaccgt gagcgccttg ataccttctt cacttaatgt ggctagcgcc agccgttgac
    11161 ctgccgccag gtagagcgtg ttttcactgc cctgcatttg ataaccctgc aacagaacga
    11221 tcggctgttt gccactggca tcaacgacgg tgagatcggt accaggattc acggtagcca
    11281 gctcgcctat gcgagaagtt ttgaaaggat aaatatgtgc gccattagtc actgaagtag
    11341 cggcctgact ttccagctca tgcgccgcgt tgtcatccat ttttatttct ggcggaaaat
    11401 gggcaatgcc tttatgacaa tcgatacagg tttcgccgcc cttttgtgct ttgttatgca
    11461 ttttctgcgc agattcactt tgcgaggcaa tatccatggc atcaaaagaa tggcaactac
    11521 ggcacgttgc agagtcagtt gcttttaatt ctttccatac tgtttcggcc atttcctggc
    11581 gatgagtttc gaacttatcg tcactgtcta ttttgccgct aacaaattca tgataaatat
    11641 ctttagatgc ttttaattta gcaaataaat aatccatccc tgactttgga atatggcaat
    11701 ctgcacattc cgcacgtatc cctttctggt ttgaaaagtg gacagttccc tgatattcct
    11761 caaaaggttt actcatcgag tggcaggaaa ggcaaaatgc tgtatccgac gttttatgta
    11821 aggctttttg cgccagcaat aacccgccgc caccaaccac aaccgctatc agcaaaaaca
    11881 ataacccaat gcgttttttc cctcgcatag attcctctaa ataaattcca gaacaccgaa
    11941 atcattgtat aattaaatac acaacgaatt aaccgcttgc ataactcggc acaacactgc
    12001 ctgaaataat cgataaagaa tatgatttat tacaatgtaa tcattaattg ctaa
//
LOCUS       DAZGTR010000132        11940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGTR010000132 DAZGTR010000000
VERSION     DAZGTR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.134"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(162..803)
                     /locus_tag="ACHLCY_004004"
                     /pseudo
     CDS             complement(162..803)
                     /locus_tag="ACHLCY_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008815333.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1004..1088)
                     /locus_tag="ACHLCY_004005"
     tRNA            complement(1004..1088)
                     /locus_tag="ACHLCY_004005"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1052..1054),aa:Leu,seq:caa)
     gene            1284..2303
                     /gene="ahr"
                     /locus_tag="ACHLCY_004006"
     CDS             1284..2303
                     /gene="ahr"
                     /locus_tag="ACHLCY_004006"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP0371169.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDTGELKPQDIEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVSTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSEQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            2433..3935
                     /gene="yjgR"
                     /locus_tag="ACHLCY_004007"
     CDS             2433..3935
                     /gene="yjgR"
                     /locus_tag="ACHLCY_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP0371170.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAIIQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(4054..5136)
                     /gene="lptG"
                     /locus_tag="ACHLCY_004008"
     CDS             complement(4054..5136)
                     /gene="lptG"
                     /locus_tag="ACHLCY_004008"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0371171.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5136..6236)
                     /gene="lptF"
                     /locus_tag="ACHLCY_004009"
     CDS             complement(5136..6236)
                     /gene="lptF"
                     /locus_tag="ACHLCY_004009"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0371172.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6299..6349)
                     /gene="ytgA"
                     /locus_tag="ACHLCY_004010"
     CDS             complement(6299..6349)
                     /gene="ytgA"
                     /locus_tag="ACHLCY_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP0371173.1"
                     /translation="MSIIILATISTTQENK"
     gene            6503..8014
                     /gene="pepA"
                     /locus_tag="ACHLCY_004011"
     CDS             6503..8014
                     /gene="pepA"
                     /locus_tag="ACHLCY_004011"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP0371174.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8147..8590
                     /gene="holC"
                     /locus_tag="ACHLCY_004012"
     CDS             8147..8590
                     /gene="holC"
                     /locus_tag="ACHLCY_004012"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP0371175.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8590..11445
                     /gene="valS"
                     /locus_tag="ACHLCY_004013"
     CDS             8590..11445
                     /gene="valS"
                     /locus_tag="ACHLCY_004013"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP0371176.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     KDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11501..>11940)
                     /locus_tag="ACHLCY_004014"
     CDS             complement(11501..>11940)
                     /locus_tag="ACHLCY_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFP0371177.1"
                     /translation="NAYDSSVYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYL
                     TVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMID
                     WQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIMPSLPFL"
BASE COUNT         2945 a         3091 c         3215 g         2689 t
ORIGIN      
        1 ataaagagaa gaatttgttt tatctgcaat acctcacttg ctacagcagc agtgatccat
       61 ccattcacga tatcaagtat tttatccata acctatcccc caaaattgaa actgtatatg
      121 aggataaaac agcataatat aaacgtggta atcgtatttt caatttcagg ctgcaatcgc
      181 tttaactcca gcgccggacg atgctgtcga ttactggaag caaccgcccc cgccatctct
      241 tgcgccggat tgtaatcaat taacccgctt tgcactgcat aacgcatgat ggccgtggtg
      301 cgctgttgaa ggcgggaagc cacttcaaga cgtccggatt tctccacggc cttcatagga
      361 attaacagat cgcgggtgcc tagttcagca atgttgcgag caccaattgc cgggaagata
      421 ttgtcctcca ggctcttttt tacacgatta gcatgatctt ccgaccattt ttggttggtt
      481 acgagccact ctctggcaac cttctcgaaa gtaataatct ctttcgcctg ctcttctttc
      541 acagcacgct tatgttcacg aggatcgata ccagcagcta ccagctttct ggcctcttcc
      601 cttttcttcc gcgcatccgc cagcgaaaca tccgggtaaa caccgaacgc catcaaatgt
      661 tgtttaccgc caaaacggaa acgaaatcgc caatactttg aaccgccagg ttttacaagt
      721 aaggacatgc cgtcgccgtc aacaagtgaa tactctttct cctcagattt cgcagagcga
      781 actttagtat ccgttagggc cataatggtc ttccttccat tggtatctcg tgagtataca
      841 agcaatatcg aatcggcaaa tatactcata agtatactca caagcctgtt gatgtgggtt
      901 gattcaggtt gacttcagtt gaggagaaaa gctggaaaag ccttgtgtag cgcggatttc
      961 aggcataaaa aaagacctca gttgaggtct atttgcatac ttttggtgcc gaaggccgga
     1021 ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac cgatttcgcc
     1081 acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg cacgccacca
     1141 tgcaagcgcc agcttgctcg cacctcgcta agtgctgaaa ttttcagcat tgcataccgc
     1201 gatgtgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac acttcccaga
     1261 ccacaccaga gaaggataaa agcatgtcga tgataaaaag ctatgccgca aaagaagcgg
     1321 gcggcgaact ggaagtttat gagtacgata ccggtgagct gaagccacaa gatattgaag
     1381 tgcaggtgga ttactgcggg atctgccatt ccgatctgtc gatgatcgac aacgaatggg
     1441 gattttcaca gtatccgctg gttgccgggc atgaggtgat tgggcgcgtg gtggcactcg
     1501 ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt ggctggacag
     1561 cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac tgcgagcaag
     1621 gcgcggtgtc aacaattatg aatcgcggtg gctttgccga gaagttgcgt gcggactggc
     1681 aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg ctgttgtgcg
     1741 gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc agccgcgttg
     1801 gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcac gcaatgggat
     1861 gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg gcgatgggtg
     1921 ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg gagcagtttg
     1981 atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt gaggcgctga
     2041 cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct gttccggcct
     2101 ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg ccttatgagc
     2161 tgcgaaagct gatgcgcttt gccgcccgta gcaaggttgc gccgaccacc gaactgttcc
     2221 cgatgtcgaa aattaacgac gccatcaagc atgtgcgcga cggtaaggcg cgttaccgcg
     2281 tggttctgaa agctgacttc tgatctcttt gccgggtgcc tgtcgcccgg cacttaaatc
     2341 tctttggaag gcgtctcgca atctgcttaa tttagcggtg aagagtgcac agccgttgcc
     2401 tatactccag gcaggaaact ggaggatatc tcatgagcga acccctgtta attgcccgca
     2461 cgccggacac agaactgttt ttactgccgg gaatggctaa ccgtcacggg ctgattactg
     2521 gcgcaacggg gacgggtaaa actgtcacgc tgcaaaaact ggcggagtct ctatcggaaa
     2581 tcggtgttcc ggtgtttatg gccgatgtga aaggcgatct gaccggcatc gcgcaggcag
     2641 gaacggcatc ggaaaaactg ctcgctcggt tgaaaaatat cggtgtcaac gactggcaac
     2701 cgcatgccaa tccggtggtg gtgtgggata tctttggcga gaagggccat ccggtgcggg
     2761 cgacggtttc ggatctgggg ccgctgttgc tggcgcggct gttgaatctc aacgatgtgc
     2821 aatctggcgt gctgaatatc atcttccgca ttgctgacga tcaggggctg ttactgctcg
     2881 actttaaaga tttgcgggcg attatccagt acatcggcga taacgccaaa tctttccaga
     2941 atcagtacgg aaatatcagc agcgcatcgg ttggtgccat ccagcgcggg ctgttgtcgc
     3001 tggaacagca aggcgcagca cacttctttg gtgagccgat gctggatatc aaagactgga
     3061 tgcgcaccga tgccaacggt aaaggcgtta tcaatatcct cagcgccgag aaactttatc
     3121 agatgccgaa actgtacgcc gccagcctgc tgtggatgat ttcagagttg tatgaacaat
     3181 tgccggaagc gggcgatctg gagaagccaa aactggtgtt tttcttcgac gaagcacatc
     3241 tgctgtttaa cgatgcaccg caggtactgc tggataagat tgagcaggtg ataaggctta
     3301 ttcgctcaaa aggcgtgggc gtctggttcg tttcgcaaaa cccgtctgat attccggata
     3361 atgtgctcgg ccagctcggt aatcgcgttc agcacgcttt gcgggctttt acgcccaaag
     3421 atcagaaagc ggtaaaagct gcggcgcaaa ccatgcgggc caatccggcg tttgataccg
     3481 aaaaggcgat tcaggaactg ggcaccggcg aggcgttgat ctcttttctg gatgcgaaag
     3541 gaagcccttc tgtggtggag cgtgcgatgg tgatcgcgcc ttgttcgcgg atggggccgg
     3601 tgacggaaga tgagcgtaat ggcttgatta atcactctcc ggtgtatggc aaatatgagg
     3661 atgaggtgga ccgggaatcc gcctatgaga tgttgcaaaa aggctttcag gccagtaccg
     3721 agcagcaaaa taatcccccc gcgaaaggga aagaggtagc ggtggatgac ggcattcttg
     3781 gtggattgaa ggatattttg ttcggcacta ccggaccacg cggcgggaag aaagatggtg
     3841 tggtgcaaac aatggccaaa agcgccgctc gccaggtgac gaatcagatt gtgcgtggaa
     3901 tgttggggag tttgctgggg gggagaagaa ggtaagtcag gcgcgatctg tccccctcac
     3961 cctaaccctc tccccaaagg ggcgagggga ccgatcgaac tcgtatttga ctttatcaac
     4021 aatgtgaaag ggagctttcg ctcccttgct tggttacgat tttctcatta acagccacag
     4081 gctgattaag aagaagctgg cgcttggcaa cagtgcgccg atgatcggcg ggatgccata
     4141 aaccaacgtc aacgggccga agatctggtc cagtacgtag aagacaaaac cgaagctgat
     4201 accggtgacc acacgcacgc ccatcggtac gctacgcagt gggccaaaga tgaacgacag
     4261 cgccatcagc atcatcaccg ccacggatag cggctggaag attttgctcc acatgttgag
     4321 ctgataacgt ccagcgtcct gaccgctcga cttcagatac ttcacgtagt tgtgcaaacc
     4381 gctgattgag agtgcatccg ggtccagcgc caccacgccc agtttgtctg gcgtgaggtt
     4441 ggttttccag gtgccgctca ccgtctgcga accggtgatc tgtttcggat tggtcagatc
     4501 agattcatca acctgcgaca gacgccagac tttatgttcc gggtcaaact tcgcagtagc
     4561 ggcatagcgt acggattgca gacgacgatt ttcgttaaag gcataaatgc tgatgccacc
     4621 taactcttcg tcacctttaa cccgctcaat gtagacgaag ttgttgccat ctttcgccca
     4681 taagccttgc tgggtagaga gcaacgagcc gccgtacatc gcctgcgcac ggtagttacg
     4741 cgccatctgc tcgccctgcg gcgcgaccca ttcaccaatc gccatcgtca gcaagactag
     4801 cggaatggcg gttttcatca ccgacagcgc aacctgcata cgggtgaaac cagaagcctg
     4861 catcaccacc agttcgctgc gctgcgccag catcccaaga ccaagcaacg ccccaagcag
     4921 agctgccatc gggaagaaga tttgcacatc tttcggcacg ctcagcaagg tatacattcc
     4981 tgcgcctaac gcgtcgtaac tcccctgccc ggcttttttc agctgatcga caaacttgat
     5041 aatgcccgac agcgacacca gcatgaacag cgtcatcatg atggtggtga aaatagtttt
     5101 accgatatag cggtcaagta cgccaaaagg ttgcatcaca ccgctccttt acgcgaaaaa
     5161 ctggcgcgca ggcggcggac cggcacggtg tcccaaaggt tgagaacaat cgccaaagcc
     5221 agataaatca ggttaacggt ccacatccac agcgtcgggt ccagcttacc tttaccgccg
     5281 ttcgatttca gggaggtctg gatcaggaag aacaacagat acagcagcat ggctggcagc
     5341 atcgatagta cgcgcccctg acgcgggtta accacgctca gcggtacgac cataagtgcc
     5401 atcataaaca cggtgaacac caacgtgata cgccagttca gttctgcgcg agcacgatcg
     5461 gtgtcagtgt tccacaatgt gcgcatgtcc atctggtcgg tatcgttcgg gtcgagcgcc
     5521 accgcctggt gaccaatgat cgcctgataa tcctggaaat ccgtaatgcg gaaatcacgt
     5581 aacagtgcag tgccttcgaa gcgcgttccc tggttgagag tgacgacctg ggagccgtcg
     5641 cgcagctggg ttaaatgtcc ggaatcggcc accaccacag aaggacgtgc attacctttt
     5701 ggtcgaattt gcgcgaggaa cacatctttg aaatcgctgc cgtcaacgct ttcgatgaac
     5761 agcaccgaac tgccattagt cgcttgctgg aattgccctt gcgccagcgc cgccatgcca
     5821 gggttcgctt tcgcttctgc taacacttca tcctgatgac gcgatgacca cggtcccgcc
     5881 cacatcacgt taaccgccgc gacgattgcc gtgaataccg caaggatcat tgccgctttc
     5941 accagaaccg ctttgctcag gccgcaggca tgcattaccg taatttcact ttcggtatac
     6001 agtttgccca gcgtcatcag cagcccgagg aacaggctta atggcaggat aagctgcgcc
     6061 atttccggca cgcccaaccc gagaagggag agcaccagat tcgccggaat atcgccgtca
     6121 accgctgcgc cgaggatcct cactaacttt tgacagaaga agatcaaaag caagatgaag
     6181 agtatcgcca gctggctttt gagcgtctcc cgcaccagat atcttatgat tatcacttta
     6241 aatacgcccg taaaaactcg tcttttgcag gattttagct tgtttcatgg cttaaacgtc
     6301 atttattctc ttgagtcgtc gaaatcgtcg ctaagataat tatactcaac ggattcacct
     6361 ctcagatttt gttctgacgt gccaatgccg taataacgtt aagattaaca cgaagtcatc
     6421 gcaacagcgg acatgagtta cgaaagcttg caattctatc tgtagccacc gccgttgtct
     6481 ttaagattca ggagcgtagt gcatggagtt tagtgtaaaa agcggtagcc cggagaaaca
     6541 gcggagtgcc tgcatcgtcg tgggcgtctt cgaaccacgt cgcctttctc cgattgcaga
     6601 acagctcgat aaaatcagcg atgggtacat cagcgccctg ctacgtcggg gcgaactgga
     6661 aggaaaaccg gggcagacat tgttgctgca ccatgttccg aatgtacttt ccgagcgaat
     6721 tctccttatt ggttgcggca aagaacgtga gctggatgag cgtcagtaca agcaggttat
     6781 tcagaaaacc attaatacgc tgaatgatac tggctcaatg gaagcggtct gctttctgac
     6841 tgaactgcac gttaaaggcc gtaacaacta ctggaaagtg cgtcaggctg tcgagacagc
     6901 aaaagagacg ctctacagtt tcgatcagct gaaaacgaac aagagcgaac cgcgtcgtcc
     6961 gctgcgtaaa atggtgttca acgtgccgac ccgccgtgaa ctgaccagcg gtgagcgcgc
     7021 gatccagcac ggtctggcga ttgccgccgg gattaaagca gcaaaagatc tcggcaatat
     7081 gccgccgaat atctgtaacg ccgcttacct cgcttcacaa gcgcgccagc tggctgacag
     7141 ctacagcaag aatgtcatca cccgcgttat cggcgaacag cagatgaaag agctggggat
     7201 gcattcctat ctggcggtcg gtcagggttc gcaaaacgaa tcgctgatgt cggtgattga
     7261 gtacaaaggc aacgcgtcgg aagatgcacg cccaatcgtg ctggtgggta aaggtttaac
     7321 cttcgactcc ggcggtatct cgatcaagcc ttcagaaggc atggatgaga tgaagtacga
     7381 tatgtgcggt gcggcagcgg tttacggcgt gatgcggatg gtcgcggagc tacaactgcc
     7441 gattaacgtt atcggcgtgt tggcaggctg cgaaaacatg cctggcggac gagcctatcg
     7501 tccgggcgat gtgttaacca ccatgtccgg tcaaaccgtt gaagtgctga acaccgacgc
     7561 tgaaggccgc ctggtactgt gcgacgtgtt aacttacgtt gagcgttttg agccggaagc
     7621 ggtgattgac gtggcgacgc tgaccggtgc ctgcgtgatc gcgctgggtc atcatattac
     7681 tggtctgatg gcgaaccata atccgctggc ccatgaactg attgccgcgt ctgaacaatc
     7741 cggtgaccgc gcatggcgct taccgctggg tgacgagtat caggaacaac tggagtccaa
     7801 ttttgccgat atggcgaaca ttggcggtcg tcctggtggg gcgattaccg caggttgctt
     7861 cctgtcacgc tttacccgta agtacaactg ggcgcacctg gatatcgccg gtaccgcctg
     7921 gcgttctggt aaagcaaaag gcgccaccgg tcgtccggta gcgttgctgg cacagttcct
     7981 gttaaaccgc gctgggttta acggcgaaga gtaagtttgc gtttaccctc accctaaccc
     8041 tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc atcttttagg
     8101 gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatttatga aaaacgcgac
     8161 gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg agcaactggt
     8221 gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtgctcatcg cctgtgaaga
     8281 tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag aaagctttgt
     8341 tccgcataat ttagcgggag aaggaccgcg cggcggtgcg ccggtggaga tcgcctggcc
     8401 gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa gctttgcaga
     8461 ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt ctctgaaaca
     8521 actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga atacggcaac
     8581 ctggaaataa tggaaaagac atataaccca caagatatcg aacagccgct ttacgagcac
     8641 tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag tttctgcatc
     8701 atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt ccagcaaacc
     8761 atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct gtggcaggtc
     8821 ggtactgacc acgcagggat cgctacccag atggtcgttg agcgcaagat tgccgcagaa
     8881 gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat ctgggaatgg
     8941 aaagcggaat ccggcggcac cattacccgt cagatgcgcc gtctcggcaa ctccgtggac
     9001 tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa agaagttttc
     9061 gttcgtctgt ataaagaaga cctgatttac cgtggcaaac gcctggtaaa ctgggatccg
     9121 aaactgcgca ccgctatctc tgacctggaa gtggaaaacc gcgaatcgaa aggttcgatg
     9181 tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagatggtaa agattatctg
     9241 gtggttgcga ctactcgtcc ggaaaccctg ctgggcgata ctggcgtagc cgttaacccg
     9301 gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct ggttaaccgt
     9361 cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg ctgcgtgaaa
     9421 atcactccgg cgcacgactt taacgactat gaagtgggta aacgtcacgc gctgccgatg
     9481 atcaacatcc tgacctttga cggcgatatc cgtgaaagtg cccaggtgtt cgataccaaa
     9541 ggtaacgaat ctgacgttta ttccagcgaa atcccggcag agttccagaa actggagcgt
     9601 tttgctgcac gtaaagcagt cgttgctgcg gttgacgcgc tcggcctgct ggaagaaatt
     9661 aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtagttat cgaaccgatg
     9721 ctgaccgacc agtggtacgt gcgtgccgat gtcctggcga aaccggcagt tgaagcggtt
     9781 gagaatggcg acattcagtt cgtaccgaag cagtacgaaa atatgtattt ctcctggatg
     9841 cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg tatccctgca
     9901 tggtacgacg aagcaggtaa cgtttatgtt ggccgcaaca aagacgaagt gcgtaaagaa
     9961 aataacctcg gtgccgacgt tgccctgcgt caggacgaag acgttctcga cacctggttc
    10021 tcttctgcgc tgtggacctt ctctacccta ggctggccgg aaaacaccga cgccctgcgt
    10081 cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt ctggattgcc
    10141 cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc gcaggtgccg
    10201 ttccacaccg tttacatgac cggcctgatt cgtgacgacg aaggccagaa gatgtccaaa
    10261 tccaagggta acgttattga cccactggat atggttgacg gtatttcgct gccagaactg
    10321 ctggaaaaac gcaccggcaa tatgatgcag ccacagctgg cggacaaaat ccgtaagcgc
    10381 accgagaagc agttcccgaa cggtattgaa ccgcacggta ctgacgcgct gcgcttcacc
    10441 ctagcggcac tggcgtctac cggtcgtgac atcaactggg atatgaagcg cctggaaggt
    10501 taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat gaacacagaa
    10561 ggtcaggatt gcggcttcaa cggcggcgaa atgacgctgt cgctggcgga ccgctggatt
    10621 ctggcggagt tcaaccagac catcaaagcg taccgcgaag cgctggacag cttccgcttc
    10681 gatatcgccg caggcattct gtatgagttc acctggaacc agttctgtga ctggtatctc
    10741 gagctgacca agccggtaat gaacggtggc accgaagcag aactgcgcgg tactcgccat
    10801 acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat tccgttcatc
    10861 accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga caccatcatg
    10921 ctgcagccgt tcccgcagta cgatgcatct caggttgatg aagccgcact ggccgacacc
    10981 gagtggctga agcaggcgat cgttgcggta cgtaacatcc gtgcagaaat gaacatcgcg
    11041 ccgggcaaac cgctggagct gctactgcgt ggttgcagcg cggatgcaga acgtcgcgta
    11101 aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagtatcac cgtgctgcct
    11161 gccgatgaca aaggtccggt ttccgttact aagatcatcg acggtgcaga gctgctgatc
    11221 ccgatggctg gcctcatcaa caaagaagat gagctggcgc gtctggcgaa agaagtggcg
    11281 aagattgaag gtgaaatcag ccgtatcgag aacaagctgg cgaatgaagg ctttgtcgcc
    11341 cgcgcaccgg aagcggtcat cgcgaaagag cgtgaaaagc tggaaggcta tgcagaagcg
    11401 aaagcgaagc tgattgaaca gcaggctgtt atcgccgcgc tgtaatcaaa acacagtaat
    11461 gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat ggtaaagaag
    11521 gcataattcc ccgtgaaatc ctggctaaga agcctttatc tggttgttct tctttattga
    11581 ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc cagtcaatca
    11641 tccatatttt cagcagtgga taagccagac cgcccgtaat cccggatatc accaccaacg
    11701 cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga atacgcccat
    11761 cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa taactggtgc
    11821 taacagcaat cccaaaataa tagattaact gcgcgattat cattttacgt tgcatttcca
    11881 taaattgctg taaattctca atatcatcat ttgcatacac agaactatca tacgcattta
//
LOCUS       DAZGTR010000133        11938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGTR010000133 DAZGTR010000000
VERSION     DAZGTR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.135"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            52..420
                     /locus_tag="ACHLCY_004015"
     CDS             52..420
                     /locus_tag="ACHLCY_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0371178.1"
                     /translation="MNAVLTELNKLGKATADSISKGLNIDLNDVIDTLWKLKNQGIVK
                     VKNGIWQAVEGVGSKTDIDSVQPVQPVQPVQNNIIGDLLRKSRKEARRAGRKQKRWEG
                     ACKALQELNKYRDLINELSE"
     gene            499..681
                     /locus_tag="ACHLCY_004016"
     CDS             499..681
                     /locus_tag="ACHLCY_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371179.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(665..1834)
                     /locus_tag="ACHLCY_004017"
     CDS             complement(665..1834)
                     /locus_tag="ACHLCY_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0371180.1"
                     /translation="MALSDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVVTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIRRPVFTQ
                     VKPYIERLLNAGFDVLFPGQEIDKPIDRSSANLYMKKLREKIDIPEWRTHDFRRSLVT
                     NLSGEGIMPHVTEKMLGHELGGVMAVYNKHDWLSEQKDAYELYADKIFWHAKQLG"
     gene            2030..2104
                     /locus_tag="ACHLCY_004018"
     tRNA            2030..2104
                     /locus_tag="ACHLCY_004018"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2063..2065,aa:Arg,seq:cct)
     gene            complement(2545..3792)
                     /locus_tag="ACHLCY_004019"
     CDS             complement(2545..3792)
                     /locus_tag="ACHLCY_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP0371181.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMVLRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(3864..4778)
                     /locus_tag="ACHLCY_004020"
                     /pseudo
     CDS             complement(3864..4778)
                     /locus_tag="ACHLCY_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
     gene            4994..6427
                     /gene="cscA"
                     /locus_tag="ACHLCY_004021"
     CDS             4994..6427
                     /gene="cscA"
                     /locus_tag="ACHLCY_004021"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP0371182.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6435..7430)
                     /locus_tag="ACHLCY_004022"
     CDS             complement(6435..7430)
                     /locus_tag="ACHLCY_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0371183.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7673..7891
                     /locus_tag="ACHLCY_004023"
                     /pseudo
     CDS             7673..7891
                     /locus_tag="ACHLCY_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            7909..9237
                     /gene="dsdA"
                     /locus_tag="ACHLCY_004024"
                     /pseudo
     CDS             7909..9237
                     /gene="dsdA"
                     /locus_tag="ACHLCY_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(9345..10883)
                     /gene="emrY"
                     /locus_tag="ACHLCY_004025"
     CDS             complement(9345..10883)
                     /gene="emrY"
                     /locus_tag="ACHLCY_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP0371184.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(10883..>11938)
                     /gene="emrK"
                     /locus_tag="ACHLCY_004026"
     CDS             complement(10883..>11938)
                     /gene="emrK"
                     /locus_tag="ACHLCY_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP0371185.1"
                     /translation="MELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGD
                     ILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNR
                     RVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAA
                     DATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKET
                     QLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRV
                     PVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDT
                     SPIEKEISNIISHNGQL"
BASE COUNT         3411 a         2872 c         2664 g         2991 t
ORIGIN      
        1 ctcaccaatc gcataacacc cccaccgaca ttaaaatatc aggagaaaaa aatgaacgcc
       61 gtactcacag aattgaacaa attaggaaaa gcaacagccg acagcatttc taaaggtctc
      121 aatattgatt tgaatgacgt cattgatacg ctgtggaagt tgaaaaacca ggggatcgta
      181 aaggtaaaaa acggcatctg gcaggcagta gagggagttg gaagcaaaac agatattgat
      241 tcagttcaac cagttcaacc agttcagcca gtgcagaaca acattatagg tgacctgcta
      301 cgtaaatcac ggaaagaagc gcgccgcgcc gggcggaaac agaaacgatg ggagggtgca
      361 tgtaaggcgt tgcaagaact gaataaatac cgtgacttga tcaacgaatt gtcagagtga
      421 acgtagcggg aatccacgtc ctttagggcg tggatgatat caggctgttg aaatacatgt
      481 agaaaaggag cgcatactat ggaagtcaac ttaatgaccg aaaaagaggt ttctgatttg
      541 ttacaaaaaa ggcgcacagc tttgtataat ttgcgaaaaa aacacggatt cccggaacca
      601 gtacttactc atccggcgcg atatagtcgt caggccgttg aacaatggct taaagccgga
      661 ggaattaacc gagctgttta gcgtgccaga aaattttatc agcatacaac tcatacgcat
      721 ctttctgttc cgacaaccag tcgtgtttat tatacacggc cataactccc ccaagttcat
      781 gccccagcat tttttcagtg acgtggggca taattccttc cccagataaa ttcgttacca
      841 gagaacgcct aaaatcatgc gttctccatt caggaatatc aattttctcc cttaactttt
      901 tcatgtacaa attagccgac gaacgatcaa taggtttatc tatttcctgc ccggggaata
      961 gcacatcaaa acccgcatta agcaatctct cgatataagg tttaacctga gtgaataccg
     1021 gacgccggat aatattcccc atttttgaat gctcttttgg ggttgtccag accaaatctt
     1081 ccatgttgaa ctcactggct gtcgcgagac gtagctcaga aagtctggcc ccccatagca
     1141 atagcaattg atgtaacacc ttgttggaag taactacttt tgagttctcc agcgccagcc
     1201 aaatttttgc cagttccgta taggtaagta cacgactccc tacatctggt tttttcccaa
     1261 tattcttaac actaagcttg agtaattcgc atgatgggat taactggcgg ctgatacacc
     1321 agttaataac tgaacgcagt tgtaacaaca aaacacgagc ctttttcttg ttgaggcttt
     1381 cttgtttgtc aaagaaacga gcccatgccg atactgggat actggcaact ggcgagcctt
     1441 caaactgtgt gtacatagtg ttgtacacga cggatctgta aagcgtctgt gtattgggtt
     1501 tcagatcagt aacatactta tcccaccact gatccaggca ttcttttaat gtcagttcgc
     1561 cgtcactggg cgcaaaataa tttttagggt taactccctt catgtacaat gcgcgcatct
     1621 caccgacgat aacacgcgcg tccttcagtg aagtagacgg atagcgcccg acagtaagac
     1681 gtactggctt accattccag cgataacggt actgaaacgt gatagttcct gctggagtaa
     1741 tccgcacact taacccgtca ccatcggtga tttcagcagg gccagagtag ggttttccgt
     1801 agaggcctcg tagtttggtg tcgctcagtg ccaatgcttt atatcctgta cacaagttta
     1861 taatgtattc tgtactcaat atgtacgcat tagcaagtga acaaaacact tttcaacgca
     1921 aaagaagata atcaaccatg aacaaaaaca aacttacacc ttgattttaa aaccataaag
     1981 acggtattat cagtcatatg gtgaacaacc aaaatcaata cgcaacaatg tcctcttagt
     2041 taaatggata taacgagccc ctcctaaggg ctaattgcag gttcgattcc tgcaggggac
     2101 accagatacc cttcaaacga aatctacctt caccccgtaa aagataggtt tggcagcaca
     2161 cttgccttat atctactcat ttttactgca acaggttgaa atctcagcac tgtcagaaag
     2221 cgctgatgac taaacagccc tgggccgggc gatgtaacca tcacacagaa tcctgatagc
     2281 gaaatatggc gtgactcgat actttactcc gcaatgcatt ccttgatgaa ttcgcaggac
     2341 cgtgatacac gggacaggtc gctgaatgac gacaatgtcc tggaaatcag cgaaccgcgc
     2401 atctgaagta catttgagcg actgtaccag aacatgaatg aggcgtttgg attaggtgat
     2461 tattagcagg gctaagcact ttagtattat tattttccgg ttgagggata gggagatatc
     2521 gacaacaacc ggaaaagttt acgtctatat tgctgaaggt acaggcgttt ccataactat
     2581 ttgctcgcgt tttttactca ggaagaaaat gccaaatagc aacatcaggc agacaatacc
     2641 cgaaattgcg aagaaaactg tctggtagcc tgcgtggtca aagagtatcc cagtcggcgt
     2701 tgaaagcagc acaatcccaa gcgaactggc aatttgaaaa ccaatcagaa agatcgtcga
     2761 cgacaggcgc ttatcaaaat ttgccacgct gtatttgaag acggatatga cacaaagtgg
     2821 aacctcaata gcatgtaaca gcttcactaa tgaaataatc caggggttaa cgaacagcgc
     2881 gcaggaaagg atacgcaaca ccataatcac aacaccgata agtaatgcat tttttggccc
     2941 tacccgattc acaaagaaag gaataatcgc catgcatagc gcttcgagta ccacctggaa
     3001 tgagttgaga taaccataca ggcgcgttcc tacatcgtgt gattcgaata aacctgcata
     3061 aaagacagga aaaagttgtt gatcaaaaat gttatagaaa gaccacgtcc ccacaataaa
     3121 tatgacgaaa acccagaagt ttcgatcctt gaaaactgcg ataaaatcct ctttttttac
     3181 ccctcccgca tccgccgcta cgcactggtg atccttatct ttaaaacaca tgttgatcat
     3241 cataaataca gcgccaaata gcgagaccaa ccagaagttg atatggggac tgatactaaa
     3301 aaatatgccg gcaaagaacg cgccaatagc atagccaaaa gatccccagg cgcgcgctgt
     3361 tccatattcg aaatgaaaat ttcgcgccat tttttcggtg aagctgtcaa gcaaaccgca
     3421 tcccgccaga taccccaggc caaaaaagag cgcccccaga attagaccta cagaaaaatt
     3481 gctttgcagt aacggttcat aaacgtaaat cataaacggt ccggtcaaga ccaggatgaa
     3541 actcatacac cagatgagcg gtttcttcag accgagttta tcctgaacga tgccgtagaa
     3601 catcataaat agaatgctgg taaactggtt gaccgaataa agtgtaccta attccgtccc
     3661 tgtcaaccct agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga
     3721 aataaaaaag agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat
     3781 attcagtgcc ataattacct acctgtcgtt aaaaaattca cgtcctattt agagataaga
     3841 gcgacttcgc cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt
     3901 gcccctttcg ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc
     3961 atttctctct catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg
     4021 aacgcatctc ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga
     4081 acttgtcctc gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg
     4141 atctcatact cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata
     4201 agtcgccatt cttcttccga gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa
     4261 cacaagcgga gcaaatgctc gtcttaccat agatcttcac gaatattagg atcgaagctg
     4321 acaaaacctc cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc
     4381 tcggcagaca acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag
     4441 tctgtcgtct ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc
     4501 ccttgatcgt tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga
     4561 tacgtgatat cgactccctc agttagcagc gttccttgca ttaacgcccc aaaaggatca
     4621 tcaccgaccc gacctataaa tccacttatt ccgcctaatc tggcgattcc caccgcaacg
     4681 ttagctggcg cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct
     4741 acgaccgcat cccctaaaac ccatactttg gctgacattt ttttccctta aattcatctg
     4801 agttacgcat agtgataaac ctctttctcg caaaatcgtc atggatttac taaaacatgc
     4861 atattcgatc acaaaacgtc atagttaacg ttaacatttg tgatattcat cgcatttatg
     4921 aaagtaaggg actatatttt tataaaagtt aacgttaaca attcaccaaa tttgcttaac
     4981 caggatgatt aaaatgacgc aatctcgatt gcatgcggcg caaaacgcac tagcaaaact
     5041 tcatgagcac cggggtaaca ctttctatcc ccattttcac ctcgcgccac ctgccggatg
     5101 gatgaacgat ccaaacggcc tgatctggtt taacgatcgt tatcacgcgt tttatcaaca
     5161 tcacccgatg agcgaacact ggggaccaat gcactgggga catgccacca gcgacgatat
     5221 gatccactgg cagcatgagc ctattgcgct agcgccagga gacgataatg acaaagacgg
     5281 gtgtttttca ggtagtgctg tcgatgacaa tggtgtcctc tcacttatct acaccggaca
     5341 cgtctggctc gatggtgcag gtaatgacga tgcaattcgc gaagtacaat gtctggctac
     5401 cagtcgggat ggtattcatt tcgagaaaca gggtgtgatc ctcactccac cagaaggaat
     5461 catgcacttc cgcgatccta aagtgtggcg tgaagccgac acatggtgga tggtagtcgg
     5521 ggcgaaagat ccaggcaaca cggggcagat cctgctttat cgcggcagtt cgttgcgtga
     5581 atggaccttc gatcgcgtac tggcccacgc tgatgcgggt gaaagctata tgtgggaatg
     5641 tccggacttt ttcagccttg gcgatcagca ttatctgatg ttttccccgc agggaatgaa
     5701 tgccgaggga tacagttacc gaaatcgctt tcaaagtggc gtaatacccg gaatgtggtc
     5761 gccaggacga ctttttgcac aatccgggca ttttactgaa cttgataacg ggcatgactt
     5821 ttatgcacca caaagctttt tagcgaagga tggtcggcgt attgttatcg gctggatgga
     5881 tatgtgggaa tcgccaatgc cctcaaaacg tgaaggatgg gcaggctgca tgacgctggc
     5941 gcgcgagcta tcagagagca atggcaaact tctacaacgc ccggtacacg aagctgagtc
     6001 gttacgccag cagcatcaat ctgtctctcc ccgcacaatc agcaataaat atgttttgca
     6061 ggaaaacgcg caagcagttg agattcagtt gcagtgggcg ctgaagaaca gtgatgccga
     6121 acattacgga ttacagctcg gcactggaat gcggctgtat attgataacc aatctgagcg
     6181 acttgttttg tggcggtatt acccacacga gaatttagac ggctaccgta gtattcccct
     6241 cccgcagcgt gacacgctcg ccctaaggat atttatcgat acatcatccg tggaagtatt
     6301 tattaacgac ggggaagcgg tgatgagtag tcgaatctat ccgcagccag aagaacggga
     6361 actgtcgctt tatgcctccc acggagtggc tgtgctgcaa catggagcac tctggctact
     6421 gggttaacat aatatcaggt ggaacaacgg atcaacagcg ggcaagggat ccgcgtcact
     6481 cttccccctt cacgaccttc aataatatgc aatgcagctt cccgcccgat aatgtcatgt
     6541 ggaagctgaa ttgtggtcag cggcggtaaa aacagatgcc cgacgccaac cagattatca
     6601 aagcccatta cggcgacatc ctgcgggatt cgtaccccct tcgccagaag aacctgataa
     6661 gccacaaagg ctgcgcgatc gttaccacat atcagaacat caaaatctgg tttgcccgat
     6721 ttgaagtggt cattgagtaa acttgcgaga tcggtgtagt gatcatcacc tgttgccatg
     6781 tgaaattgtt tcacctcagc cagatctcgt ccagcatcac gccaggcctg ctcaaatccc
     6841 tgccgacgat accctgttgc caacgcactt tccggtagcc agaagcataa cggttgacga
     6901 tagcccgccg cgagcaaatg ctgtgttgat tcatattgtg cagtgtaatc atcagggata
     6961 taactgggta acgctgggtc atccgccaca cagttcgcca atacaatatt ttcaccatac
     7021 agagactcag gcagcgtgat atgtcgcagc cccattgtag tatagataat gccatccgga
     7081 cggtgggcaa gcagctgacg tgccgcgcgg gcagcgtcat cttcagaaaa aatattgatt
     7141 aaaaaactat tccagccgaa ctcgctggcg gtttgctcaa tggcaagcag aatatcaaca
     7201 gagaaaggag tggtagccgt gtcctgcgcc agcacggcga gagtcgacgg cttacgtcct
     7261 tgagcgcgca tcttacgggc ggaaagatca ggaacataat tcagggtctg gattgcctgc
     7321 aatacgcggt cacgcgttgc aggacgcaca gattctgcat tatgcatcac ccgggagact
     7381 gtcatcatcg acactcccgc caggcgtgcg acatccttta atgaagccat acccaagccg
     7441 tttgccgtaa aacgggcact gtagcagaaa cagacgtcac tggcgagatc caacgatatt
     7501 ctcaacgccc tatcacccga cacggcaata caataaaaaa taacaataat tgccggataa
     7561 ttgtactcaa ttccgcctct gttctcgcca acgagtaacc atataggcaa gtaagtaaaa
     7621 catgccaact ggcatcgcca acctggagaa gaaaatataa atatggatca ttgtgctgcc
     7681 gctgtatccc gacatcagcc cggaaattat tgcgattgct atcggttcag gtgcaattgg
     7741 ctgcacgatc gttacggact cgcttttctg gctagtgaag caatattgcg gcgctacgct
     7801 caatgaaaca tttaaatact atacgacagc gacatttatc gcttcagtcg tcgctctggc
     7861 gggcacattc ctgctgtcat ttatcatcta agcgcaaaga gacgtactat ggaaaacgct
     7921 aaaatgaact cgctcatcgc ccagtatccg ttggtaaagg atctggttgc tcttaaagaa
     7981 accacctggt ttaatcctgg cacgacctca ttggctgaag gtttacctta tgttggcctg
     8041 accgaacagg atgttcagga cgcccatgcg cgcttatccc gttttgcacc ctatctggca
     8101 aaagcatttc ctgaaactgc tgccactggg gggattattg aatcagaact ggttgccatt
     8161 ccagctatgc aaaaacggct ggaaaaagaa tatcagcaac cgatcagcgg gcaactgtta
     8221 ctgaaaaaag atagccattt gcccatttcc ggctccataa aagcacgcgg cgggatttat
     8281 gaagtcctgg cacacgcaga aaaactggct ctggaagcgg ggttgctgac gcttgatgat
     8341 gactacagca aactgctttc tccggagttt aaacagttct ttagccaata cagcattgct
     8401 gtgggctcaa ccggaaatct ggggttatca atcggcatta tgagcgcccg cattggcttt
     8461 aaggtgacag ttcatatgtc tgctgatgcc cgggcatagc aaaaagcgaa actgcgcagc
     8521 catggcgtta cggtcgtgga atatgagcaa gattatggtg ttgccgtcga ggaaggacgt
     8581 aaagcagcgc agtctgaccc gaactgtttc tttattgatg acgaaaattc ccgcacgttg
     8641 ttccttgggt attccgtcgc tggccagcgt cttaaagcgc aatttgccca gcaaggtcgt
     8701 atcgtcgatg ctgataaccc tctgtttgtc tatctgccgt gtggtgttgg cggtggtcct
     8761 ggtggcgtcg cattcggact taaactggcg tttggcgatc atgttcactg cttttttgcc
     8821 gaaccaacgc actccccttg tatgttgtta ggcgtccata caggattaca cgatcagatt
     8881 tctgttcagg atattggtat cgacaacctt accgcagcgg atggccttgc agttggtcgc
     8941 gcatcaggct ttgtcgggcg ggcaatggag cgtctgctgg atggcttcta tacccttagc
     9001 gatcaaacca tgtatgacat gcttggctgg ctggcgcagg aagaaggtat tcgtcttgaa
     9061 ccttcagcac tggcgggtat ggccggatct cagcgcgtgt gtgcatcagt aagttaccaa
     9121 cagatgcacg gtttcagcgc agaacaattg cgtaatgcca ctcatctggt gtgggcgacg
     9181 ggaggtggaa tggtgccgga agaagagatg aatcaatatc tggcaaaagg ccgttaataa
     9241 cgtttcaacg cagcatcgca atcctttccc tgggtgagcg atgctgccga tggcgcagac
     9301 ttaagatccc cgctcttacc cgctataacc ccctcctttt atcatcaccc aacgcctttc
     9361 gctgtaaacg gcggtttcgc aaaccaaacc aaaaccgtaa gtaagataaa agcaatagcc
     9421 gccatacgga aaatctcatt tgcagaaata gaaagtgact gctgggttat ttcattatta
     9481 atttcattaa gaactcctga aagcgaacca tagtatttat ccataatttg tgacgatgaa
     9541 ttaaacacgg ggttaaattg atcgatggtt gctgtcaact gactatggtg taacgattcg
     9601 cgtcgtcccc acagcgtcat tgtcaacgac gtaccaactg atcctgacaa ggtacgaaaa
     9661 aaattactca tactcgaggc attggcaaat ttattatctg gcaagcctga aaacgaaatc
     9721 gttgttaagg gtaaaaagaa acaggcaacg gcgaatccct ggaaaaactg tggcaaaatg
     9781 atgcctgtaa aatcaatcgt tggcataaat gtcacagaac gccagtaata gcaaaccgca
     9841 tacatcaaaa aactaaatgt cactaacaac cgcatgtcta ttttgttgcc ataacgtcct
     9901 atcaaaggtg aaattaatag tggcatgatg ccgatgggcg cataagcaag tccggcccat
     9961 atcgcattat accccatcgt ttcctggagt aactgcggca taaggacgat cgctccagag
    10021 taaaataaat acgcgcatgt gatactcaca ataccaatgg tgaagttacg ggacttaaac
    10081 aaactgagat caagaatcgg gttctctgag gtcgactccc aaatgactaa agagatcaga
    10141 gaaataactg atactactgt taatattatt attgtactcg agttgaacca atccagatcg
    10201 cgccctttat caagcataat ttgcaagcca ccaacaccga gcactaacag ggtcagtcct
    10261 ggtagattca ttttgaccgg tgaagtttca gtttctcttc ctttaagtaa ggttaagcat
    10321 aatgtcagga cgataatccc catagggaca ttgattaaaa atatccaacc ccagctaaag
    10381 ttatcgcaaa tataaccgcc caatatcggc ccacatatcg gagcgataat cacggtcatt
    10441 gaccataatg ccagagcaaa tgttcttttt tctggtggat aattccttaa taataaactc
    10501 tgtgacagtg gaattaacgg ccccgccatt aatccctgaa cgactctaaa aaatatcagc
    10561 acatcaagat tggtcgataa actacacatt aaggaagaca gcgaaaaaaa agtgactgaa
    10621 agtaaaaata accttaattc gcctattctt tgtgccagcc tgccggtaac agggatcgca
    10681 atggcatttg ctacgccaaa cgaggtgata acccaggtgc cttcgtctgt tgatgctccc
    10741 agaaagccag atattgtcgg tattgcgacg ttagaaatag tggaatccaa catttgcata
    10801 aacgtcgcta atgacaatgc aatagtaacg caccataacg tcccaccggt taatggtgcc
    10861 ggagtcgatt tagtgattgc cattaaagtt gtccattatg cgaaataatg ttgctaattt
    10921 ctttttctat cggactggta tcgataacta aagccttact ggtataagcc ggcatggacg
    10981 tcacggttga agccagatca ggcatctcag caatgtcttc gttcttcgta tcaatagttg
    11041 cagtcatcga taaaccaatg cgtaaggggt gttccatgag ttcttttgga tcaagagaaa
    11101 cttcaaccgg tacacgctga acgattttga tccagttccc tgtcgcattt tgtgcaggta
    11161 ataaggagaa cgcattgccg gttcccatat tgatccctgt cacccgacca tgaaacacaa
    11221 cattttcacc ataaagatcg ctgataatat tgactgattg accaatccgt acatccgtga
    11281 gttgtgtttc tttaaagttg gcattaaccc acatttgacg tgccggtacg acagccatta
    11341 acgattgtcc gggactcact gtttcgccga cctgaacact ccgctgggca atatagccag
    11401 taaccggact ccttatatcc gtacgtttaa gcgccaacca ggcttcttta gttgcatccg
    11461 ccgcttccac gacttgtggc tgacggttta atggtgtgtt cattactaaa gctttattcg
    11521 ctttataagc ctggatagcg gcattcagtg ccgctttgct acttattaac gtatctttgg
    11581 tatgctccag cgtttctttt gaaataaccc cctgcttcgc taacggtact cgacggttat
    11641 aatcttctaa agattgttga tactgaatac gtgctgaagc gacttcggca ctgtattgtt
    11701 tatcctgtaa gtacagttta ttcgtttgcc gaacaatatt tgccagatta tttttagctt
    11761 tattgagagc gatagtggca tcagttttat ccagtgaaac taaaatgtca ccttgtcgaa
    11821 cgtagttcgt atctttatga ttaacgacag tgacactacc tgagacttgt gcagaaattg
    11881 gatctgcatt ccccgtgaca taggcgtcat ctgtactaat catgtcttct aattccat
//
LOCUS       DAZGTR010000134        11804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGTR010000134 DAZGTR010000000
VERSION     DAZGTR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.136"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..513
                     /locus_tag="ACHLCY_004027"
     CDS             <1..513
                     /locus_tag="ACHLCY_004027"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP0371186.1"
                     /translation="AFDGGGIFASIIFITNPIIINFILGMLAEFIYSNTKTNNKLLNK
                     AIKMLAPIVAVISVWGMLSPSMWMGEMQWAIPCFGLVTSLSLLEKSGVSFEFPSLVKI
                     GAMSFSIYLIHPIIIELLSQKYFVVFWQDGFTKFSVIILITVFAARIMYETIEIPSQK
                     LARKLISKIR"
     gene            complement(554..2491)
                     /locus_tag="ACHLCY_004028"
     CDS             complement(554..2491)
                     /locus_tag="ACHLCY_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFP0371187.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIIINAAGYPVYNGQIAKFVTVQGHSMAVYDAYGSQQFY
                     FPNVLKYDPDQLRQELAGPNGYLLIPSMDQHIKIQQWREEGDIRGWGAIDGEFNDAAV
                     SAALDSESPSVKLGGVGFVSKLRSPINHKSNKVMHSGSLNFQFDGGTQQEKSGILMAN
                     ISNAKVIDVDITGTLDGGIRGYGGSNIVIDGVNVHDIGISTLSGECGIGIWFGDYANY
                     DVQTDGLLIQNCNIKNIGGVGIQRGDGILVYNAKNFKVRYNTIITTNRMAIAAGSDTR
                     QFEIHGNYIGDTLLAGIDIEPDEGYTASNFKIYNNNIIGFAARYFTQGSGVGQTFGID
                     THANTSYGKVYKNTLSAGQYGTEAFHIGNHADEIEVTDNNLIGGAVVIPLFIKTYDGS
                     GSKHIKINRNRAKGTCKSFADVAMSEDVYISENVFSGNSSNDSYFLRASTIAGLNVDY
                     NRSSNTTNFIKSGDAGNTSSVKVTNNNASTLSDGIDMLTSGSMTGFIASGNTILCPAS
                     NKGISLEVAGSGSISDISLRGNIIYNATTKIYVSPAATGWDMLTKNTRFDLSGVQNGT
                     QLFELSRNRVTQFLNNAWYDG"
     gene            complement(2670..3605)
                     /locus_tag="ACHLCY_004029"
     CDS             complement(2670..3605)
                     /locus_tag="ACHLCY_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0371188.1"
                     /translation="MNSIAILEAVNTSYVPFNGQQIITAMAAGVAYVAMKPIVENLGM
                     SWSTQQTKLMKQISKFNCVHMNMVAADGKLRKLLCLPLKKLNGWLFSINPEKVRADIR
                     DKLIQYQEECFSVLHDYWTKGHVFNPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPK
                     EKQAKAMITMWSSLKSHFGCSYKEISEEQFTEALSLAARVPLEGEFIGKQEKKTNELS
                     AKEANSLVWLWDYANRSQALFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLI
                     NHTRDVDINEPDGPTNLSAWMRLKNKELPPSVHNY"
     gene            complement(3674..3835)
                     /locus_tag="ACHLCY_004030"
     CDS             complement(3674..3835)
                     /locus_tag="ACHLCY_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0371189.1"
                     /translation="MKGMSKMPQFNLRWPKEVLDLVRKVAEENGRSVNSEIYQRVMES
                     FKKEGRIGA"
     gene            3926..4177
                     /locus_tag="ACHLCY_004031"
     CDS             3926..4177
                     /locus_tag="ACHLCY_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0371190.1"
                     /translation="MARDDPHFNFRMPMEVREKLKFRAEANGRSMNSELLQIVQDALS
                     KPSPVTGYRDDAERLADEQSELVKKMVFDTLKDLYKKPT"
     gene            complement(4194..4613)
                     /locus_tag="ACHLCY_004032"
     CDS             complement(4194..4613)
                     /locus_tag="ACHLCY_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371191.1"
                     /translation="MKRIIGVVAGAILLSGCATIVGDETQLVQVNSNPSGASFKVKDE
                     SGVIVAQGKTPQGVTLAKSDGSYFGKKSYQITMEKDGYEPVTLPIKANANGWYIGGNL
                     VFGGLIGWLAVDPFNGGMYTLKPKEANASLIPSTKQD"
     gene            4751..5119
                     /locus_tag="ACHLCY_004033"
     CDS             4751..5119
                     /locus_tag="ACHLCY_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001334102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371192.1"
                     /translation="MFLFISFGATAECWVVGDMRGISYSERNNFHPEEDGFSGTFIIK
                     TNGEDASITYSGTDAGGMAYKALSKNSIIGIGANGETQRVIDSWVIHPTGTVLMSKTI
                     SGYGNMDSTKAFVGKVKRKC"
     gene            complement(5144..6982)
                     /locus_tag="ACHLCY_004034"
     CDS             complement(5144..6982)
                     /locus_tag="ACHLCY_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP0371193.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGAAIDDYFAGQVVQQQTVNQANN
                     APVREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDKPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTPAYERAINNAANAIRPLFATEATQQ"
     gene            complement(6982..8388)
                     /locus_tag="ACHLCY_004035"
     CDS             complement(6982..8388)
                     /locus_tag="ACHLCY_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFP0371194.1"
                     /translation="MDTWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNAKELARVGVDPNNVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSLNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(8398..9090)
                     /locus_tag="ACHLCY_004036"
     CDS             complement(8398..9090)
                     /locus_tag="ACHLCY_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFP0371195.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            complement(9093..9548)
                     /locus_tag="ACHLCY_004037"
     CDS             complement(9093..9548)
                     /locus_tag="ACHLCY_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HFP0371196.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            complement(9548..10249)
                     /locus_tag="ACHLCY_004038"
     CDS             complement(9548..10249)
                     /locus_tag="ACHLCY_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0371197.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVINQGADVGA
                     IAGKANQAGQGAYDAQVKNDEQDVELADHDARITANTKAINLLEVRLTTAEGKIVVLR
                     SDVDYLLDEVIDIQAHLVTVDQRLDGVESDVSDIKSDYVSKTVTESQSLASPLDVKTS
                     YSVDGIQVVGARNTGWTAATGTPLLGSFNANQSYTVGTTYTQSEVAALATGLQQARQR
                     ILALETALRLHGLID"
     gene            complement(10242..10775)
                     /locus_tag="ACHLCY_004039"
     CDS             complement(10242..10775)
                     /locus_tag="ACHLCY_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD4 domain-containing protein"
                     /protein_id="HFP0371198.1"
                     /translation="MLSENAKDIEGYEGLYAITNDGRVYSHSRVNLRGRLIKGRWLKH
                     NHNVNGYKYVCLYKDGVKKNILIHKLVASHFVGGFAEGLQVNHIDGDKYNNNYLNLEW
                     VTPSGNISHSYGLESRGNVKGERNGNSKISNDDVIKIKEMVANGFPQCEVAKLFGIHN
                     SKVSRIVNGKAWRHVNG"
     gene            complement(10797..>11804)
                     /locus_tag="ACHLCY_004040"
     CDS             complement(10797..>11804)
                     /locus_tag="ACHLCY_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019073 - viral DNA genome packaging
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein"
                     /protein_id="HFP0371199.1"
                     /translation="LGSVRDITRLRGRYAWSKDGTDSWFITDLEDESHPDRYSAQYRA
                     ESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVGAALYVAQPSLMVQKGIAGTYC
                     KTPFADSYAFISNPATGAPSVYIIGSGQASPIASASIEKILRSYTADELADGVMESLR
                     FDAHELLIIHLPRHVLVYDASSSANGPQWCVLKTGLYDDVYRAIDFIYEGNQITCGDK
                     LESVTGKLQFDISSQYEKQQEHILYSPLIKADNALINDLELETSGGVCDRIDKIFISA
                     TTDGISYGREQMVVLQKPFVYDNRVLWRKVGRVRRLIGFKFRVIAKGPVTLSGLSIRV
                     T"
BASE COUNT         3017 a         2826 c         2479 g         3482 t
ORIGIN      
        1 gcatttgatg gcggtggaat atttgcaagt attattttca taacaaaccc aataataata
       61 aactttatat tagggatgct tgctgagttt atttatagca atacaaaaac caacaacaaa
      121 cttttaaaca aagcaattaa aatgttagct ccgatagttg ccgtaatatc agtctggggg
      181 atgctatcac cttcaatgtg gatgggtgaa atgcagtggg cgataccttg ctttggtctt
      241 gtaacctctt tgtcactact ggagaagtct ggggtttctt ttgaatttcc aagtcttgta
      301 aaaattggag caatgtcatt ctcaatatat ctaatacacc caataattat cgaattatta
      361 agccaaaaat attttgttgt tttttggcag gatggattta ctaagttctc agtcatcatt
      421 ttgattacag tttttgcggc aagaataatg tatgaaacaa ttgagattcc atcacaaaaa
      481 ctagccagaa agctcatatc aaaaataaga tgaaaaactg cgataccatt aaatttgtgg
      541 tatcgcaggt cgcctagcca tcataccaag cattgttaag aaactgtgta actctatttc
      601 ttgatagttc aaatagctga gtgccatttt gaactccaga cagatcaaac ctggtatttt
      661 tggttaacat atcccatccc gtggcagctg gtgatacata tattttggtt gtagcattat
      721 aaattatgtt gccacggaga ctaatgtctg aaatagaacc tgaacctgct acttccagtg
      781 aaattccttt gttggatgct ggacataaaa tagtgttgcc agaagcaata aaaccagtca
      841 tggaacccga cgttagcatg tctataccat ctgaaagggt agaagcgtta ttattagtga
      901 ccttcacgct tgacgtgttt ccagcgtcac cagacttgat gaaattggtt gtgttgcttg
      961 accggttgta gtcaacatta agccctgcta ttgttgacgc tctcaggaaa taactatcat
     1021 tagaactatt gccagagaat acattttcag aaatataaac atcttcagac atggctacat
     1081 ctgcaaatga cttacacgtc ccttttgccc tgttgcggtt tattttgatg tgtttactac
     1141 cactaccatc atatgtcttg atgaataatg ggattacaac agcaccgcca atcaggttgt
     1201 tatccgtaac ttcaatctca tccgcatggt ttcctatatg aaatgcttct gttccatact
     1261 gtcctgcaga caatgtgttt ttatacactt tgccatatga ggtattagca tgagtatcta
     1321 taccaaaggt ttgaccaaca ccagatcctt gagtgaagta tcgtgcagca aatccgataa
     1381 tattgttatt gtatatcttg aaatttgaag cagtgtaacc ttcgtcaggc tcaatatcta
     1441 taccagctaa tagcgtatca cctatgtagt ttccatgaat ctcaaactgc ctagtatcac
     1501 ttccagctgc aatagccatc ctattggttg taatgatggt gttatacctt accttgaaat
     1561 ttttcgcgtt ataaaccagg atgccatccc cacgctgtat ccctacacct ccaatatttt
     1621 tgatattaca attttgaatt aacagcccat ccgtctgtac atcgtagttt gcataatcac
     1681 cgaaccatat tcctatgccg cactcaccgg atagcgttga aattccaata tcgtgaacat
     1741 tcacgccgtc tataacgata ttgctgccac cgtacccacg aattcctcca tccagggtgc
     1801 ctgttatatc aacatcaatc actttggcgt tggagatgtt agccataagt atcccggatt
     1861 tctcttgctg agttccgcca tcaaattgaa agtttaatga cccgctatgc atcactttat
     1921 tacttttgtg gttaataggt gagcgaagtt ttgaaacgaa tccgacgcca ccaagtttaa
     1981 cgctaggaga ttcagagtct aatgcagcgg atacagcagc atcgttgaac tctccatcaa
     2041 tagcacccca tccgcgaatg tcaccctctt ctctccactg ctgaatttta atatgctgat
     2101 ccatcgatgg gattagaaga tatccatttg gacccgctag ctcttgtctt aattggtcag
     2161 ggtcatactt cagcacatta ggaaaataga actgctggga accatacgca tcataaacag
     2221 ccatagaatg gccttgtaca gttacgaatt tggcaatctg tccgttatat acagggtaac
     2281 cagcagcgtt aatgatgatt ggttgcgaaa cagggacgtg agagccgtct tcgttttcca
     2341 cataaacctg aatctggttt tcaggattta ccgggtccgt gtcaatttta ccgatataaa
     2401 ttttgccatt ggctacggct ttaaaagaac gcgccatagt gaagagttgc gaaggcatag
     2461 atacaatcac attggctgta atgtctgtca tttaatttgc tccagataca aggaatcgcc
     2521 gcagcgtggc tacggtgagt atttgtttgc tttttgcact acactttttg tgtagtgcta
     2581 tctctcaagg ccatagccgc tgagtagcta cggtgaattt tgggcataaa aaaacccagc
     2641 cgaagctggg tcgttgcgtt ggttatctgt cagtagttat gtactgaagg aggtaattct
     2701 ttattcttaa gtctcatcca tgcggaaaga ttcgttggtc cgtctggctc attaatatca
     2761 acatctcgtg tgtggtttat taaaacgtct ctcgccattc cgataacata cgagaactca
     2821 tgaccgtagt cgtagcatct gccggaatag ttcgattgaa tttgttttag cgccggatac
     2881 agttcgcgga ataatgcctg tgagcggttg gcataatccc ataaccatac aaggctgttt
     2941 gcttcttttg cagaaagctc gttggttttc ttctcttgtt tgccaatgaa ctcaccttca
     3001 agtggaactc gagctgcaag tgatagtgct tcggtaaact gctcctcact gatttctttg
     3061 tacgaacatc caaaatggga ttttagtgac gaccacatgg tgatcatcgc cttagcctgt
     3121 ttttcttttg gcagagactg accgcgactc atgacgagtt gtttaatggc ttcctgctgt
     3181 tcagtggtga ttttacccgg caacgccttt ttagctttgc gtgggttaaa tacatgacct
     3241 ttagtccagt agtcatgcag cacgctaaag cattcttcct ggtactgaat cagtttatca
     3301 cggatgtcag cacgaacttt ctcagggttg atgctgaaca gccatccatt taacttcttc
     3361 aaaggaaggc agagtagctt acgaagcttc ccatcagcgg caaccatgtt catatgaaca
     3421 cagttgaatt tgctaatctg cttcatgagt tttgtttgct gcgttgacca gctcattcca
     3481 aggttttcaa cgattggctt catcgcaaca tatgcaactc cggcagccat ggcggtgata
     3541 atttgctgac cgttgaatgg tacataagag gtgttcactg cttctaaaat tgctatacta
     3601 ttcacgttgg tttttctcca cggatttact gacaaccgaa gccctgactg ttcccgcagt
     3661 tggggcttca actttacgcg ccaatgcgcc cttccttctt aaagctttcc attactctct
     3721 gataaatctc agagttaaca gaccgaccat tctcttccgc caccttgcgg accaaatcca
     3781 atacttcttt aggccaccgc aaattgaact gcggcatctt gctcattcct ttcatattca
     3841 cctcacaata taggtccacc gtggacctat tgagaatata gtagagtgct tctatcatgt
     3901 caatacacta acttggagtg atggcatggc tagagatgat ccgcacttta acttccgtat
     3961 gcctatggaa gtaagggaga aattaaaatt cagggcggag gcgaatggga gatcaatgaa
     4021 ctccgagttg ttacaaatcg tccaagatgc tctatcaaaa ccatcgcctg tgactggata
     4081 tcgcgacgat gcagaacgac tcgctgatga gcagtcagag cttgttaaga agatggtgtt
     4141 tgatacgctg aaggatttgt acaaaaaacc cacctgaagg tgggtcctat ttattagtct
     4201 tgctttgttg atggtataag agatgcgttt gcctcttttg gcttcaaggt atacatccca
     4261 ccattaaaag gatctacagc aagccaacca attaacccac caaacacaag gtttccacca
     4321 atataccagc cattagcatt ggctttgatt ggcagggtta ctggttcgta cccatccttt
     4381 tccatagtga tctggtagct ctttttgcca aaataactac catctgactt ggcaagagtt
     4441 actccttgcg gggtcttacc ttgcgcaaca atcacgcctg attcgtcttt taccttaaag
     4501 ctcgcaccgg aaggattgct gttcacttgc acaagctgcg tttcgtcacc aacaatagtt
     4561 gcgcacccag ataacaatat agcgccagca acgacgccaa taatcctctt catatcaatt
     4621 tccatattta aaaaccggaa acatcctaat gacaaaccat tcaaatgtga agtaggcaaa
     4681 agatgtttac ttttttcatg gtatcctgct caaaactaag gaggttggcg tgaagcaatt
     4741 tcttactgct atgttcttat tcatatcttt tggggctaca gcagagtgct gggtcgttgg
     4801 agatatgcgc ggaataagct attcagaacg aaataatttc catccggaag aagatggttt
     4861 tagtggaaca ttcatcatta agacaaacgg tgaagatgcc agcatcacat attctgggac
     4921 agatgcgggc ggcatggctt acaaagcatt gtctaaaaac tccatcatag gaatcggcgc
     4981 gaatggcgaa actcaacgcg ttatcgactc atgggtaata catcctactg gaacagtttt
     5041 aatgtcaaaa accatttccg gttatggaaa tatggattca accaaagctt ttgttggaaa
     5101 agtaaaaaga aaatgttagc gattgaatcc aatttcccat acgttactgc tgtgttgcct
     5161 cagtagcaaa cagcggtctg atggcattcg cagcgttatt tatcgctctt tcataggctg
     5221 gtgttcctgc tttggtgttt gccaaacgaa gaagcatatt ccttgctgct ttggactcat
     5281 acaagcgcat cattgcacca aaaccagcct caagccccat tgatacgcca agggtcgcag
     5341 ttgcgccaat cgtccttatc ctgttggctt gcgattgccc cgtctgagtt actacatttg
     5401 cggtgtctga ccttgctgtt tgctgtagaa cttcatgaag agcatcaagc tctttcatgt
     5461 gctttccaga aaaaatagtg ttgtaaattt caccgcctga ctgagatttc agcttattaa
     5521 cttcagtgat gaacttggct ggagagtccc cggccttttc cgctattttg ctgacgtaag
     5581 ctgcacgcat agcatctttc cccttatcat ccagtgcgct ccagattcgt ttcacgtcag
     5641 atggttttct gcttaataca acggtattta taagttcagg actggcttca ctgcttgcct
     5701 tgttgagctt gttggcaatg tttttattaa gcaccttatt ataaacgttt gcataatcgg
     5761 aatttgcttt aaggtatttt gctgcgtctg atgcaccgag gtttttagca actgcgttac
     5821 gaaggtcttt tgacattgca ttctctacca tattggtagc tgcttttgcc tggttgggga
     5881 agaccatagc atctccctga acattagatc taaatgctgt tctgtgctga cgcaagagat
     5941 caaacgtaac atccaaatca gttgcagggt ttgctaattc ttcacgtagg ttacgcaagg
     6001 atgtaagcag gctttgattg gcagacgtcc caagccgttc ctgtcttgcg atcgctgtat
     6061 tcagagcatt catggtattt gtggtatcaa ctgcggcatt acccatttta ttggtgacgt
     6121 cattgataac agcgccagcg gcatccttcc gcccccttaa cgtggtggtc agagatttca
     6181 ccacatcatc agggttgtac tcaccaaaac ggtcaaaata attgcttacc agcttactcc
     6241 gcgttgcata ttgctctgct cgctttgagc ccgtcccgag caaagccccc tcggcatcct
     6301 gagtaaggcc gcgagtgaaa gcatttttcg gcgggataac atcagatgtc attggtgtca
     6361 cgcccatcga ttctgatgtg gcaattttct tcgccacttc tggcgcaata tcacctttta
     6421 tagccgttat tccacgccct attccctttg ctgctgcgga aagaacaccc tgagcggcaa
     6481 ggttaactcc ggcattttta gctgcatttt gtgcgaaatc gcctttctga tttgcggcct
     6541 ctgccagcga tccaatagcc atgcttcctg ccgttccaac tcctggaact aaatacccac
     6601 caattgtttc accggcttgt gcgtatgggt ctgtcggttt gtctactgga cgataaacat
     6661 catccaaaac ttttggccca ccaagcccct gactgattgc attaatcaga cttgcgccac
     6721 cctgcaatac gtcaaatggt atgtttacca gaccacgacc agcctgttct gcaatttgcc
     6781 ctgcactttg accaccagtg agccaatcgc cagcttgttg catcaatgat ggttcttcac
     6841 gtactggtgc attattggcc tgattaactg tttgttgctg aacaacctga ccagcaaaat
     6901 aatcatcaat ggcggctcca atatcttccg tgctcgtacc atcagggaag gtaaatgtct
     6961 taccgtttgc agttactttc atcattccac cgtaaattga atgcctgatt ttgaggtata
     7021 tgatccaacc tgattccgtg gttctcctga aggtgtcgaa tcttgtgctg gcgctgcgtc
     7081 agtattcaat gacatatacc gcttaacggc actccccaat gattcacctt ttttaacatc
     7141 caaccccaat atctgaccgc cattacgcga ttgtccagga ttgccattcg cgctcatcca
     7201 ctcagcttta aactcattaa actgcgcgtt tcgtcgctca aggtttgcca ttgcatcaag
     7261 ccatcttgcg accgtctcag ggttatccat gtcagttggc gcaccctgcc gaacgatctc
     7321 aacgtcttta tccgttgctg ggccgggagg taggaattta agaacctgac tgttaacaag
     7381 ggcattttgg cggatgcgca aatcacgcaa tgtcgtatcg cttccggtaa gttttgcgaa
     7441 catgttctgt gcgttaccga acaaacctgt cgttggtttt tctgctctga actgttgagc
     7501 aagcgcactc atagaattgg ctgagtttga tgatgctgtg gcattgttta cagccgtctc
     7561 gatgcctttt tccatgttta ctgacagctt aggtgcttca ctaatcaact gctgagcctt
     7621 ttcctgcgct tgctgcatct taaacccgaa ctcttgctga tccagagcca agcgttgtgc
     7681 tgcgatattg tgcccagtca ttgctgactg ataggaaagg ttttgccctc tcgcctgaag
     7741 tgcttcacca gcctgattgc tgcggattgt ctctgccagt ctgcctcggt caatctcacg
     7801 accagccatc ttgtcctgaa cattgaagta gtcaatcgga ccgagagcag ccatcccaag
     7861 gtgatcaaca aactcaccaa atcctgaagg attctgctga tacatctgag caacgttatt
     7921 agggtcaaca ccgacgcgcg ccagctcctt ggcgttgttt tgcagccatg attgcattgc
     7981 ttctggagac gaggccgcaa ggcgtgcgcc agccgctaag gttccgatag aattgcgctg
     8041 gtcttcatca atgaatccca tgcctttacg aacggattca atctggtctg gatattgagt
     8101 agccaactga cgcaaagcac cgcgatcacc agacgcataa gcattagcgt atgcctgctg
     8161 aaattctttc tgccgctgag cctgcttttc ctgctgaaac acccccgcaa tacctgaaag
     8221 gccttgcaaa gcagtcagcc caacattgtt agcgcctgaa cgctcaatat cattgttctg
     8281 cctaataagt tgaagcgtat tgccgatgtc atttacgctc ggagcgtttg agttgacgcc
     8341 gccgatacca gccaacaatc cgccatttga tccttgccaa gtatccatga ttacccctta
     8401 aaacaacgag ccaagcaatc cgataccagc accaatgcca gcgccccaag gtgttgatgt
     8461 tcccaaaaga ctggcaagac ctgcaccggc aatcgcacca gacgtgccac cgctaattgc
     8521 agtctgaaga cttgatggtt tattggcatt agcagcggca agagctgcgc tttgctgtgc
     8581 aatgctgctc atgttgttgg cgtacgtctg cccggcgttt gcctgacctt gcagcgcacc
     8641 aagcccaacg tttgccagat tgttgtaatt gctcatctgg tttgataacc aagactgacc
     8701 gagagtcggc gcgatcgtag ccagttgatt gcttgtggct gtcgaaccaa gtcctcccgt
     8761 agcctccgca gcagcaagac tctggtaacg agcctgacct gcaaggtctt tatactgctg
     8821 agagttgtaa tactgattaa gtgcctgccc ctgaccttct aaactggaaa gattctgaag
     8881 ctggttaaca tactgctcag caagcggcgt gaacggagca aggtttttca tgatcgtctg
     8941 ccactgctga ttttgcaggt ctgcggcata cttctgagct tctgctgcat actttgcgct
     9001 tttatcagag ctgccacctt tcccgccttt ttcagggcaa taaggttcct cgccgcgcag
     9061 ttttctgccc agcttaaatg catataacat ggctatctcc cgtgattcag gaagtcgatt
     9121 agttcttcgc gtgtggcgct gtaaaacgtc acgtcatcca cgcctttgaa gtatttcttg
     9181 atggttccta cacgcttaag gccaatcatt gcgcagtaca tctgcccgtg gcggaatttg
     9241 cgtgcagcaa atgatgtgac gcactgaacg gtggtgttgg tcaaaatgta tcgccagaac
     9301 gccagcccta tttccttgct gaatccgcga atctctggca ggtacatagc gtggcaatca
     9361 aaggtcagcg gctgaatctc ctgatagtaa acaattccgc cgaactgccc gtgcacgttc
     9421 acctcaaagt aacggcattc aggcttgtag tcgtatccat caccgttgtt gctcccggca
     9481 ataatgtcag ggtgatttcc gactgcttcg atcaagtcga tgtttcgcgt tggtttgaat
     9541 gtaatcatca gtcaatcagc ccatgtaatc taagtgctgt ttcaagcgcc agaatacgct
     9601 gccgcgcctg ctgcaaacct gtagcgagag ccgcgacttc ggattgtgtg tacgtagtgc
     9661 cgaccgtgta tgactggtta gcgttgaatg agccaagaag aggtgtacct gtggctgcag
     9721 tccatccggt atttcttgct ccaacaacct gaattccatc aactgaatat gatgttttta
     9781 catccagcgg tgacgcaaga gactgcgatt cggttacggt tttcgatacg taatcactct
     9841 taatgtcaga tacatcgctt tctacgccat ccagtctttg gtcaacagtg accagatgcg
     9901 cctgaatatc gataacctca tccagcaagt aatcaacatc gctacgcagt acgactatct
     9961 tcccttcggc ggttgttaac ctgacctcaa ggagatttat cgcttttgtg tttgcggtga
    10021 ttcttgcatc gtggtcagcc agttcgacat cctgttcatc gtttttcacc tgagcatcgt
    10081 aagcgccctg accagcctga tttgccttcc cggcaattgc gccgacatca gccccctgat
    10141 taatgacata cagcaggtaa gactggctga atatattgcg tggaaggact gatgtgtcga
    10201 gccgtgtagc ctgaatgatt accggcacat tgagattcga atcagccatt aacatgcctc
    10261 catgccttgc catttacaat tctactaacc tttgaattgt gaattccaaa taatttagca
    10321 acttcacact gaggaaatcc attggcaacc atttctttta tctttatgac atcatcatta
    10381 gatattttag aattaccatt cctttctcct tttacattac ctctcgactc caatccatat
    10441 gagtgtgaga tatttcctga tggagtaacc cattcaagat ttagatagtt attattatat
    10501 ttatcaccat ctatatgatt tacctgtaac ccttcagcaa atccaccaac aaagtgagat
    10561 gcaacaagct tatgtattag tatgttcttt tttacgccat ccttatataa acacacatac
    10621 ttatatccat ttacgttatg attatgcttc agccacctac ccttaataag acgtcccctc
    10681 aaatttactc ttgaatgcga gtaaactcta ccatcattag tgatggcgta cagtccttca
    10741 tatccttcga tatcttttgc gttttcactt aacatatcta ctccttgagt ttcgatttat
    10801 gttacacgaa tagaaaggcc tgataatgtg acaggacctt ttgcaataac cctgaattta
    10861 aatccaatga ggcgtctaac tcgaccaacc tttcgccata aaacgcgatt gtcatataca
    10921 aatggttttt gtaatacgac catttgctca cgaccgtaac taattccatc tgtagtggct
    10981 gatataaata ttttatctat tctatcacac acgccgcccg atgtttctaa ttcaaggtca
    11041 tttattaaag cgttatctgc ctttattaga ggggaatata aaatatgctc ttgttgtttt
    11101 tcgtattgtg aagatatatc gaattgcaat ttcccggtca cggactccag cttatcgccg
    11161 cacgttatct gattgccttc gtaaatgaag tcgatagcgc ggtacacatc gtcatacagg
    11221 ccagttttca acacacacca ttgcggacca ttggcgcttg aagatgcgtc gtacacgaga
    11281 acatggcgcg gaaggtggat aatcagcaac tcatgagcat caaaccgcag cgattccatc
    11341 acaccatcag ccagttcatc agcagtgtag gagcggagga ttttctcaat gctcgcgctg
    11401 gcgattggtg atgcctgacc tgagccgatg atgtatacag acggcgcacc cgttgccgga
    11461 ttgctgatga acgcatacga atcagcaaac ggcgttttgc agtaagttcc ggcaatgcct
    11521 ttctgcacca tcagcgatgg ctgtgcgaca tacaaagcgg caccaacagt ggttgcacca
    11581 gtcaaggaaa aatattcaat cgtcgatgaa ccaaagcaga cgatgaagtc tcgccatgtt
    11641 ccgatgccga tgatgccgtc aggctgcgat tctgcgcgat attgtgcgct gtatctgtca
    11701 gggtgcgatt cgtcttcaag gtcagtgata aaccatgaat cagtgccgtc ttttgaccac
    11761 gcataacgcc cgcgtaagcg cgtaatgtca cgaacagaac ctaa
//
LOCUS       DAZGTR010000135        11659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGTR010000135 DAZGTR010000000
VERSION     DAZGTR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.137"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            342..1481
                     /gene="wza"
                     /locus_tag="ACHLCY_004041"
                     /pseudo
     CDS             342..1481
                     /gene="wza"
                     /locus_tag="ACHLCY_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
     gene            1487..1936
                     /gene="wzb"
                     /locus_tag="ACHLCY_004042"
     CDS             1487..1936
                     /gene="wzb"
                     /locus_tag="ACHLCY_004042"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP0371200.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRSLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRDAFDAVYALLERSARQWAQALNAEQGKQ"
     gene            1933..4095
                     /gene="wzc"
                     /locus_tag="ACHLCY_004043"
     CDS             1933..4095
                     /gene="wzc"
                     /locus_tag="ACHLCY_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP0371201.1"
                     /translation="MTEKVKQSAAPVTGNDEIDIGRLVATVVEARWWVLGITTAFALC
                     AAVYTLFATPIYSADALVQIEQNTGNSLVQDIGSALSNKPPASEAEIQLIQSRLVLGK
                     TVDDLNLDIAVTKDTFPIFGAGWDRLMGRQNETVNVTTFTLPKAMNEQTFTLNVLNDK
                     SYQLVSDGGFSARGQVGKVLEHDGVTMLVEAIHASPESQFTVSKFSTLGMINTLQNNL
                     MVTETGKDTGVLSLTFTGEDREQIRQILDSITRNYLQQNVERKSEEAAKSLAFLAKQL
                     PEVRNRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLF
                     TKAHPAYRTLLEKRQALEDEKSKLNGRVTAMLKTQQEIVRLTRDVESGQQVYMQLLNK
                     QQELKITEASTVGDVRIVDPAIAQLGVLKPKTALIILGSIILCLMLSVVGVLLRSLFN
                     RGIESPLALEEHGISVYASIPLSEWQKARDNVQTIKGVKRYKQSQLLAVGNPTDLAIE
                     AVRSLRTSLHFAMMQARNNVLMLTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGKGDISSSAKLTSIPHFDLIPRGQVPPNPSELLMSER
                     FGELIAWASSHYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIQVKGVILNSIFRRASGYQDYGYYEYEYTSDAK"
     gene            4168..5010
                     /gene="wcaA"
                     /locus_tag="ACHLCY_004044"
     CDS             4168..5010
                     /gene="wcaA"
                     /locus_tag="ACHLCY_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP0371202.1"
                     /translation="MTTENPLISIYMPTWNRQQLAIRAIKSVLRQDYPHWEMIIVDDC
                     SSSYEQLQQFVEELNDPRVSYTHNSTNSGACAVRNQAIMQANGQYITGIDDDDEWTPN
                     RLSVFLQYRHQLVTRAFLYANDYVCEGEVYSQPTSLPLYPKSVYSRRRFYKRNIIGNQ
                     VFTWAWRFKACLFDTTLKAAQDYDIFLRMVVAYGKPWKVKEATQILHVNHGEMRITSS
                     PNKFSGYFQFYRKHKGKFDRASKKYQLFTLYQIRNKRMNWRTLLTLLSVRNSKRLADG
                     LRGR"
     gene            5013..5498
                     /gene="wcaB"
                     /locus_tag="ACHLCY_004045"
     CDS             5013..5498
                     /gene="wcaB"
                     /locus_tag="ACHLCY_004045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP0371203.1"
                     /translation="MLEDLRANGWSLRPCCMVAAYRIAHFCSVWRKKNIINNLWAAPV
                     LVMYRLITECLFGYEIQAAASIGRRFTIHHGYGVVINKHVVAGDDFTLRHGVTIGNLG
                     ANDPSCPVIGNSVELGANVTLLGDVAIGDNVTIGAGSVVVDSVPDNTLVVGEKARVKV
                     I"
     gene            5498..6720
                     /gene="wcaC"
                     /locus_tag="ACHLCY_004046"
                     /pseudo
     CDS             5498..6720
                     /gene="wcaC"
                     /locus_tag="ACHLCY_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
     gene            6695..7912
                     /gene="wcaD"
                     /locus_tag="ACHLCY_004047"
     CDS             6695..7912
                     /gene="wcaD"
                     /locus_tag="ACHLCY_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP0371204.1"
                     /translation="MSRSIRICSYLVLPLLYLLVNVKLAQLGESFPITIVTFLPALLL
                     LFVERINIKKLMIALGVGGGLTAFNYLFGQSLDASKYVTSAMLFVYTVVIIGMVWSIR
                     FKTISPHNYIKILRFFWLVVGLVVGLAAVEMAQIILSGGSSLMEVISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKSDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKDAIKKKLPLAIISLSVFLVGVIFAFPYIATRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLLNVDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVILALWYMGKVMKMMINAFGDNQNYRVQLYLFTPLSLFFTGSVFSPEYAFLIV
                     CPFILRKALNIAR"
     gene            7918..8664
                     /gene="wcaE"
                     /locus_tag="ACHLCY_004048"
     CDS             7918..8664
                     /gene="wcaE"
                     /locus_tag="ACHLCY_004048"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP0371205.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAGDISFEWIVVDGGSS
                     DGTSAFLEGLSGEHRLRFVSEPDNGIYDAMNKGIAMARGRFALFLNSGDILHADAAHF
                     IRQLKMQSDQTMVIGDALLDFGNGNKIKRRAKPGWYIYHSLPASHQAIFIPLSGLNVW
                     HYDLQYKISSDYALAAQLYKSGYAFKKLSGLVSEFSMGGVSTTNNLELCRDAKKVQRQ
                     ILHMPNFWTGLSEYLRLRTTAKTKTLYGKT"
     gene            8674..9228
                     /gene="wcaF"
                     /locus_tag="ACHLCY_004049"
     CDS             8674..9228
                     /gene="wcaF"
                     /locus_tag="ACHLCY_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP0371206.1"
                     /translation="MQDLSGFKVPGGFRGGGAIKVQLWWAVQATLFRWSPQVMYRWRA
                     FLLRMFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDAVLYTLGDITIGAHAVISQ
                     KSYLCTGSHDYASQHFDINATPIVVGAKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVTRERVEKVTP"
     gene            9253..9810
                     /locus_tag="ACHLCY_004050"
                     /pseudo
     CDS             9253..9810
                     /locus_tag="ACHLCY_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            9883..10140
                     /locus_tag="ACHLCY_004051"
                     /pseudo
     CDS             9883..10140
                     /locus_tag="ACHLCY_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            10208..11080
                     /locus_tag="ACHLCY_004052"
                     /pseudo
     CDS             10208..11080
                     /locus_tag="ACHLCY_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
     gene            11217..11659
                     /locus_tag="ACHLCY_004053"
                     /pseudo
     CDS             11217..11659
                     /locus_tag="ACHLCY_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2978 a         2768 c         3090 g         2823 t
ORIGIN      
        1 tgcattattg gagagccaat attcaattac cataatgtca ccttatattc ggaagttaat
       61 ttccgcaata accctttctc atcaacgact gtatgttgag agggttttaa aaattaagct
      121 aaatcagtgt attggtagct aaaaagccag gggcggtagc gtgtctggat gcctgaaaga
      181 ccagtctgaa ttgttctgtc aatagcctgc ggatgaaatc aattttttta ggactgatgc
      241 cagttaaatt tttattcaat taactgcaag gtaaatgtgc atggcgacat tgctattaat
      301 agtgcacagg ataattactc tgccaaagtg ataaataaac aatgatgaaa tccaaaatga
      361 aattgatgcc attattggta tcagtaacct tgataagcgg ttgcacagta cttacgggca
      421 gcaatatgtc gacgatgggt aaagacgtca tcaaacagca ggacgctgat ttcgatctcg
      481 acaaaatggt gaatgtttat ccgttgaccc cgcgcctgat tgatcaatta cgtccacgcc
      541 cgaatgtagc gcgccccaat atgcccctgg aaagtgagat cgcgaattac cagtatcgcg
      601 tcgggccggg ggacgttctt aatgtcaccg tctgggatca cccggaactc accacgccag
      661 ccggtcagta ccgcagctcc agcgacaccg gcaactgggt acagcctgac ggcaccatgt
      721 tctacccgta tatcggcaaa gttcacgtgg tggggaaaac gctcgcttaa atccgcagtg
      781 atattaccgg gcgcttagcg acgtacatcg ctgacccgca ggtggacgtt aatatcgccg
      841 ccttccgctc gcaaaaaccg tatatctccg gtcaggtgaa taaatccggt caacaggcga
      901 tcaccaacgt accactgacc attctcgacg ccatcaacgc cgcaggtggc ctgaccgaca
      961 ccgctgactg gcgcaacgtg gtgctaacac acaatggtcg tgaagaacgc atttctttgc
     1021 aggcgctgat gcaaaacggc gatctcaatc agaaccgact gctttatccc ggcgatattc
     1081 tctacgtgcc gcgtaatgat gatctgaaag tgtttgtgat gggtgaagtt aaaaaacaga
     1141 gcaccctgaa agtggacttt agcggcatga ccctgactga agccctgggc aatgctgaag
     1201 gcatcgacat gaccacctcc aacgccagcg gcatctttgt cattcgtccg ctgaaaggcg
     1261 agggcgggcg taacggcaag attgccaata tctatcagct ggatatgtcc gatgccacgt
     1321 cgctggtgat ggcgacagaa ttccgcctgc aaccttatga cgtggtgtat gtcaccaccg
     1381 caccggtttc ccgctagaac cgtctgatca atcagttgct gccaactatt agcggtgtcc
     1441 gttacatgac ggatacagcc agcgacattc ataactggta atcgtcatgt ttaacaacat
     1501 cttagttgtc tgtgtcggca atatttgccg ttccccgacg gcggaacgct tactgcaacg
     1561 ttatcacccg gagctgaaag tggagtccgc tggactcggc gcgctggtcg gtaagggcgc
     1621 tgatcctacc gctatcagcg tcgccgcaga acatcaactg tcactggaag gtcactgtgc
     1681 ccgtcaaatc agccgcagcc tgtgtcgcaa ctacgacctg attttgacca tggaaaagcg
     1741 ccatatcgaa cgcttatgcg agatggcacc tgagatgcgc ggcaaagtga tgctgtttgg
     1801 tcactgggat aacgaatgtg aaattcccga tccgtatcgt aaaagccgtg atgcgtttga
     1861 cgcggtgtac gcattacttg aacggtctgc ccgccaatgg gctcaggcac tgaacgcaga
     1921 gcagggaaaa caatgacaga aaaagttaaa cagtctgctg cgccggtaac cggcaatgat
     1981 gaaatcgata ttggtcgtct ggttgcaacg gtggtggagg cccgctggtg ggtattaggc
     2041 attaccacgg cttttgcatt gtgtgccgcg gtctacacac tgttcgccac acccatctac
     2101 agtgcggacg cgctggtgca aattgagcaa aataccggaa attcactggt gcaggatatt
     2161 ggctcggcgt tgtcgaacaa acctccggcc tcggaagcgg aaattcagct catccagtcg
     2221 cgtctggtat tagggaaaac ggtagacgat cttaacctcg atatcgccgt taccaaagat
     2281 acattcccta tttttggcgc gggctgggac cggctgatgg gccgccagaa cgaaacggtt
     2341 aatgtgacca ccttcacgtt gccaaaggca atgaatgaac agaccttcac gctcaatgtg
     2401 ctgaatgaca aaagctatca actggtgagt gacgggggtt ttagcgcccg ggggcaagtc
     2461 ggcaaggtgt tggaacatga tggcgtgacg atgctcgttg aggcgattca tgccagcccg
     2521 gagagccaat ttaccgtcag caaattctcc acgctgggca tgataaacac cctgcaaaat
     2581 aacctgatgg tgacggaaac gggcaaagac accggcgtat tgagcctgac ctttaccggc
     2641 gaagaccgcg agcaaatccg ccagatcctc gacagtatta cccgcaacta tctgcaacaa
     2701 aacgtggaac gtaaatccga agaggccgcc aaaagtctgg cgtttctggc aaagcaactg
     2761 cctgaggtac gtaaccggct tgacgttgct gaaaacaagc tgaacgcctt tcgtcaggat
     2821 aaagactccg tggatttgcc gctggaagcc aaagcggtgc tggattcgat ggtgaatatc
     2881 gatgcgcagc tgaatgaact gaccttcaaa gaagcggaga tttccaagct gttcaccaaa
     2941 gcgcatccgg cgtaccgcac gctgctggaa aaacgccagg cgctggaaga tgagaaaagt
     3001 aagctgaacg ggcgcgtaac ggcgatgctg aaaacgcagc aggaaattgt tcgtctgacg
     3061 cgtgatgtgg agtccggtca gcaggtctat atgcagttgc tgaacaagca acaggaactg
     3121 aagattaccg aggccagtac ggttggcgat gtgcgcattg tcgatcctgc gattgcccag
     3181 cttggcgtgc tgaagccgaa aacggcgctg attattctcg gcagtatcat tctttgtttg
     3241 atgctttctg tggtgggcgt actgctgcgt tctctgttta atcgcggtat tgaaagcccc
     3301 ctggcgctgg aagagcatgg tatcagcgtg tatgctagta ttccgctatc tgaatggcaa
     3361 aaagcgcgtg ataacgttca gaccattaaa ggtgtgaaac gctacaagca aagccagctg
     3421 ttggccgtgg ggaatccaac ggatctcgcc atagaagcgg tccgcagcct gcgtaccagt
     3481 ctgcactttg ccatgatgca ggcgcgtaac aacgtattga tgctgaccgg cgtcagccca
     3541 tcgattggta aaacgtttgt ctgcgccaac ctggcggcag tcatcagcca gaccaacaaa
     3601 cgcgtgctgt tgatcgattg cgacatgcgc aaaggctata cccacgaact gctggggacc
     3661 aataacgtca acggcctgtc tgagatcctg attggtaagg gggatatctc atccagcgcc
     3721 aaactgacat cgattccgca ttttgatctg atccctcgcg gtcaagtccc tcccaatcct
     3781 tccgagctgt tgatgagcga gcgtttcggc gaactgattg cctgggccag ctcgcactat
     3841 gacctggtgc tgattgacac cccaccaatt ctggcggtga ccgacgcggc gattgttggc
     3901 cgtcatgtcg gcaccacgct gatggtggcg cgttatgcgg tcaacacgct gaaagaggtg
     3961 gaaaccagcc tgagtcgctt cgagcaaaat ggcatccagg tgaaaggggt gatcctcaac
     4021 tcgatattcc gccgtgccag cgggtatcag gattacggct actacgaata cgaatatacg
     4081 tcggatgcga aataaatcac caaccctgcc ggatgcggcg aaaacgcctt atccggccta
     4141 caaaagcgta tctatccgga aacgaaaatg acgacagaaa acccgctaat ttcaatttat
     4201 atgccaacct ggaaccgcca gcagctggcc attcgggcga ttaaatcggt gttgcgccag
     4261 gactaccctc actgggagat gatcattgtg gatgattgct cttcatcata tgagcagttg
     4321 cagcagtttg ttgaggaact caacgatccg cgtgtgtcgt atacgcataa ttccaccaac
     4381 tccggggcct gcgcggtgcg taatcaggcc attatgcagg ccaacgggca atacattacg
     4441 gggattgatg atgatgatga atggacgccg aaccgcctga gcgtgttcct gcagtatcgg
     4501 catcaactgg tcacacgggc gtttctgtat gccaatgact atgtctgtga gggcgaagtg
     4561 tattcgcagc cgaccagtct gccgctctat ccgaagtccg tatattcgcg ccgcaggttt
     4621 tataaacgca acatcattgg taatcaggta tttacctggg catggcggtt taaagcatgc
     4681 ctgttcgata ccacgctgaa agcggcgcag gattacgaca tcttcctgcg catggtggtg
     4741 gcgtatggca aaccgtggaa ggtcaaagag gcgacgcaga tcctgcatgt gaatcacggc
     4801 gagatgcgta ttacctcatc gccgaataaa ttctccggtt actttcagtt ttaccgcaag
     4861 cacaaaggca aatttgaccg ggccagcaag aaatatcagc tgtttaccct ctatcagatc
     4921 cgcaataaaa ggatgaactg gcgcacgctg ctgacgctgc tttctgttcg taacagtaag
     4981 cgtctggccg acggactgcg aggacgctaa ttatgctgga agatttacgc gcgaacggct
     5041 ggagtctgcg cccctgctgc atggtggcgg cctatcggat tgcccatttt tgctccgtct
     5101 ggcgtaaaaa aaatatcata aacaacctgt gggctgcccc ggtgctggtg atgtatcgcc
     5161 ttatcaccga atgcctgttt ggctatgaaa ttcaggctgc tgcatcaatc ggtcgtcgtt
     5221 tcaccatcca tcatggttat ggggtggtta tcaacaaaca cgtggtggcg ggggatgatt
     5281 ttaccctacg ccacggcgtc actatcggca atcttggtgc caacgatcca tcctgtccgg
     5341 tcatcggtaa cagcgttgag ttgggggcca atgtcactct gctcggcgac gttgctattg
     5401 gcgataacgt gacgattggc gcggggagcg tcgtggtgga tagcgttccg gacaatacgc
     5461 tggtcgtggg cgagaaggca cgggtgaagg taatataatg aagatattac agtttaatgt
     5521 gcgactcgca gaaggtgggg cggcaggtgt ggtgttagat ttgcatcttc gcgcgctgga
     5581 aaaagggctg tcgtcacggt ttgtctatgg ctatggctat ggcaaaggcg gtaaaaaagc
     5641 gccagtcatg cgaaataccc acaggttatc aaatatacgc cgcgcctgac gtcggcggct
     5701 aacattgcgc tgtttcgttt tgcaaatcgc gacatgttcg gcaatctcga caagctatat
     5761 cgcacggtta cccgcacctg cggcccgctg gtactgcatt tccatgtgtt gcacagctac
     5821 tggttgaacc tggaagaggt ggtgaccttt tgccaaaaag tgaaggcgca taaaccggat
     5881 atcaccttcg tctggaccct gcacgatcac tggagcgtga ccgggcgctg tgcctttacc
     5941 gatggctgcg aaggctggag aacgggctgt cagaaatgcc caacgctcag caactatccg
     6001 ccggtaaaag tcgataaagc ccatcagcag ttgcccggta agcggcagat gttccgtgcc
     6061 atgctggcgc tgggctgcca gtttatctcg cccagtcaac atgtggccga cgcattcaac
     6121 atcctgtatg gcgtgggacg ctgccgcgtt atcaataatg gcattgatgt ggcaaccgaa
     6181 gccattcttg ccgaactacc cgcgatgcct gcggagagcg gtcggccgaa aattgccgtg
     6241 gtggctcatg acctgcgcta tgacggcaaa accagtcaac aactggtgcg agcgattagc
     6301 gcgcttgggg acaaaattga gctgcatacc ttcggcaaat tttcgccatt tgcgggagat
     6361 aacgtcatca accacggttt tgagagcgat aagcgcacgt tgatgagtgc gcttaaccag
     6421 atggatggac tggtgttcag ctcctgtgtc gataactacc cgctgatttt gtgtgaagcg
     6481 ctttccatcg gcgtaccggt aattgccacg cacagcgatg ccgcgcagga agtgctggcc
     6541 aaatcaggcg gcaaaacgtt cagcgaagcg gacgtattgc agctggtgca gctgaaaaaa
     6601 tcagacattg cgcaagcggt atttgacacg tcccttaccg cattcagtca gcgcagtcgg
     6661 gtggcataca gtgggcaaca gatgctggag gaatatgtct cgttctatca gaatctgtag
     6721 ctatctggtg ctaccgctgc tttatctgct ggttaacgtc aaactcgccc agcttggcga
     6781 gagcttcccg ataactatcg tcaccttctt acccgctctg ctgttgctgt ttgtcgagcg
     6841 catcaatatc aaaaaattaa tgatcgcgct gggggtaggg ggcgggctga cggcgtttaa
     6901 ctacctgttc ggtcaatcac tggatgccag taagtacgta acgtcagcga tgctgtttgt
     6961 ttataccgtg gtgatcattg gcatggtgtg gagtattcgc ttcaaaacca tttcaccgca
     7021 taactacatt aagattttgc gttttttctg gctggtggtg ggactggtgg tggggttagc
     7081 tgcggttgaa atggcgcaga taattctcag cggcggcagc agtttaatgg aagttatttc
     7141 gaaatatctt atttacagta acagctatgt tctgaacttt attaagtttg gtgggaaacg
     7201 tactactgcg ctctatttcg aaccggcatt cttcgctctg gcgttaatct caatttggct
     7261 cagcatcaaa cagtttggta tcaaaacacc taagtccgat gctatgattc tggcagggat
     7321 aatattatca ggatcgtttt caggggtaat gacgtttatt ttgttttatc tcctggagtg
     7381 ggcgttccag tacctgaata aggatgcgat aaaaaagaag ctgccgctgg cgataatctc
     7441 attaagcgtg tttttagttg gggtgatatt tgcttttccg tacatcgcaa cgcggcttgg
     7501 tgatttagga actgaaggtt cgtcatcgta ttatcgtatc gtagggccat tagtgatggt
     7561 cggttattcg ttactgaacg ttgatggcgt agtgagattt ggctcacttt atgaatacgt
     7621 tgcgtcattc ggaattttta acggtgcaga tgtcggtaaa acaatagaca atggtttgta
     7681 tctgctgatt atttattttt catggttcgc ggtgatatta gccctgtggt acatggggaa
     7741 ggtcatgaaa atgatgatca atgcgtttgg cgataatcag aattatcggg tgcagcttta
     7801 tctttttaca ccgttgtcac tgttttttac aggttcagta tttagtccgg aatacgcatt
     7861 tttaattgtg tgtccgttta tattgcgcaa ggcgctgaat atcgcgaggt aactcacatg
     7921 ctgctcagta tcattaccgt cgcctttcgc aatctggaag ggatcgtcaa aactcacgca
     7981 tcgctggcac atctggcgca agcgggtgat atcagttttg agtggattgt tgttgatggc
     8041 ggctcctcgg atggtacgtc ggcgtttctt gaagggctta gtggggaaca ccggttacgg
     8101 tttgtcagcg agccggataa cggcatctat gatgcgatga acaaaggcat cgcgatggcg
     8161 cgcgggcggt tcgcgctgtt cctcaactcc ggggatattt tgcatgcgga cgcggcacat
     8221 tttatccgcc agctaaaaat gcagagcgac caaaccatgg tcattggcga tgcgttgctg
     8281 gatttcggca atggcaacaa aattaagcgc cgggcaaaac cgggttggta tatttatcac
     8341 agcctgcccg ctagtcatca ggcgatattc attccgctct caggactgaa tgtctggcat
     8401 tatgatttgc aatataaaat atcgtctgat tacgcgttgg cagcccaatt atacaagtcc
     8461 ggttacgctt ttaaaaagtt gagcgggttg gtatcggagt tttctatggg cggagtgtca
     8521 acgacgaata atctggaatt gtgtcgggat gccaaaaaag tacagcgcca gatattacac
     8581 atgccaaatt tttggaccgg tttgtcagaa tatttacgtc tgcgcacaac cgctaaaaca
     8641 aaaaccttat atgggaaaac gtaagggcat attatgcagg acttaagcgg atttaaagtg
     8701 cctggaggtt ttcgcggcgg tggtgctatt aaagtgcaat tatggtgggc ggtgcaagca
     8761 acattatttc gctggtcacc gcaggtcatg tatcgctggc gtgcgttttt attacgaatg
     8821 tttggtgcaa aaataggaaa aaacgtagtc attcggcctt cagtaaaaat tacttacccg
     8881 tggaaattaa ccctgggaga ttatgcctgg gtgggtgacg acgcggtttt atatacgtta
     8941 ggtgatatta cgattggcgc ccatgcggtt atttcgcaaa aaagttattt atgcaccggg
     9001 agtcatgatt atgccagtca acattttgat attaacgcca cgccgattgt ggttggcgct
     9061 aaatgctggt tggcgacaga tgtctttgtc gcgcctggcg tgacgattgg tgatggcacc
     9121 gtcgttggag cacgaagcag cgtatttaaa tcgctgccag caaatgttgt ttgtcgggga
     9181 aatcccgcag tagtcacgcg cgaacgcgtc gagaaagtta ctccctgagg ggatcactga
     9241 agaggttata acatgtcaaa agtcgctctc attactggcg taaccgggca ggatggttct
     9301 tacctggcag aactcctgct ggaaaaagga tacgaagtac acggtattaa gcgtcgcgct
     9361 tcatcattca acaccgaacg tgtggaccac atctaccagg atccgcattc aggcaatccc
     9421 aaatttcatc tgcactatgg tgatttgacg gattcatcca atctgacccg tattttaaaa
     9481 gaggtccaac cggatgaggt ctataacctg ggcgccatga gtcacgtcgc ggtgtcgttt
     9541 gactcaccgg aatacaccgc ggacgtcgat gcaattggta cgttgcgttt gctggaggct
     9601 atccgcttcc tcggtctgga aaagaagacc cgtttctatc aggcctccac ctcagaactg
     9661 tacggcctgg tacaggaaat cccgcagaaa gagaccacgc cgttctaccc gcgctcgccc
     9721 tatgcggtag ctaagctgta cgcctactgg atcaccgtca actaccgcga gtcttacggg
     9781 atgtatgcct gtaacggcat tctgttcaac tgaagtggca cactgaattt ggccacctga
     9841 acagaggtga tatgctcacc tcagaacaac acaggtgctc caatgaaaaa aagaaatttt
     9901 agcgcagagt ttaaaagcga atccgctcaa ctggttgttg accagaaata cacggtggca
     9961 gatgccgcca aagctatgga tgttggcctt tccacaatga caagatgggt caaacaactg
    10021 cgtgatgagc gtcagggcaa aacaccaaaa gcctctccga taacaccaga acaaatcgaa
    10081 atacgtaagc tgaggaaaaa gctacaacgc attgaaatgg agaatgaaat attaaaaaaa
    10141 gccgctgtag attcaattgg tcaacgcaac agttatgtga aaacatgggg ttgcggaggt
    10201 tttttgaatg agacgaacat ttacagcaga ggaaaaagcc tctgtttttg aactatggaa
    10261 gaacggaaca ggcttcagtg aaatagcgaa tatcctgggt tcaaaacccg gaacgatctt
    10321 cactatgtta agggatactg gcggcataaa accccatgag cgtaagcggg ctgtagctca
    10381 cctgacactg tctgagcgcg aggagatacg agctggtttg tcagccaaaa tgagcattcg
    10441 tgcgatagct actgcgctga atcgcagtcc ttcgacgatc tcacgtgaag ttcagcgtaa
    10501 tcggggcaga cgctattaca aagctgttga tgctaataac cgagccaaca gaatggcgaa
    10561 aaggccaaaa ccgtgcttac tggatcaaaa tttaccattg cgaaagcttg ttctggaaaa
    10621 gctggagatg aaatggtctc cagagcaaat atcaggatgg ttaaggcgaa caaaaccacg
    10681 tcaaaaaacg ctgcgaatat cacctgagac aatttataaa acgctgtact ttcgtagccg
    10741 tgaagcgcta caccacctga atatacagca tctgcgacgg tcgcatagcc ttcgccatgg
    10801 caggcgtcat acccgcaaag gcgaaagagg tacgattaac atagtgaacg gaacaccaat
    10861 tcacgaacgt tcccgaaata tcgataacag acgctctctg gggcattggg agggcgattt
    10921 agtctcaggt acaaaaaact ctcatatagc cacacttgta gaccgaaaat cacgttatac
    10981 gatcatcctc agactcaggg gcaaagattc tgtctcagta aatcaggctc ttaccgacaa
    11041 attcctgagt ttaccgtcag aactcagaaa atcactgacc cgcctgggat taagattact
    11101 gcattccgca gttaagcggc gtaactagac tggccccctg aatctccaga caaccagtat
    11161 cacttaaata agtgatagtc ttaatactag tttttagact agtcattgga gaacagatga
    11221 ttgatgtctt agggccggag aaacgcagac ggcgtaccac acaggaaaag atcgcaattg
    11281 ttcagcagag ctttgaaccg gggatgacgg tctctctcgt tgcccggcaa catggtgtag
    11341 cagccagcca gttatttctc tggcgtaagc aataccagga aggaagtctt accgctgtcg
    11401 ccgccggaga acaggttgtt cctgcctctg aacttgctgc cgccatgaag cagattaaag
    11461 aactccagcg cctgctcggc aagaaaacga tggaaaatga actcctcaaa gaagccgttg
    11521 aatatggacg ggcaaaaaag tggatagcgc acgcgccctt attgcccggg tatggggagt
    11581 aagcttagtc agccgttgtc tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg
    11641 aaccgatgac tggatggat
//
LOCUS       DAZGTR010000136        11398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGTR010000136 DAZGTR010000000
VERSION     DAZGTR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.138"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(295..507)
                     /gene="yncH"
                     /locus_tag="ACHLCY_004054"
     CDS             complement(295..507)
                     /gene="yncH"
                     /locus_tag="ACHLCY_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFP0371207.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININTNKNIRQVC"
     gene            complement(583..1200)
                     /gene="yncG"
                     /locus_tag="ACHLCY_004055"
     CDS             complement(583..1200)
                     /gene="yncG"
                     /locus_tag="ACHLCY_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP0371208.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            1466..2964
                     /gene="ansP"
                     /locus_tag="ACHLCY_004056"
                     /pseudo
     CDS             1466..2964
                     /gene="ansP"
                     /locus_tag="ACHLCY_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
     gene            complement(3079..4140)
                     /gene="yncE"
                     /locus_tag="ACHLCY_004057"
     CDS             complement(3079..4140)
                     /gene="yncE"
                     /locus_tag="ACHLCY_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP0371209.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4382..6484
                     /gene="pqqU"
                     /locus_tag="ACHLCY_004058"
     CDS             4382..6484
                     /gene="pqqU"
                     /locus_tag="ACHLCY_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP0371210.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSTAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6520..7185)
                     /gene="mcbR"
                     /locus_tag="ACHLCY_004059"
     CDS             complement(6520..7185)
                     /gene="mcbR"
                     /locus_tag="ACHLCY_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP0371211.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7383..8420)
                     /gene="curA"
                     /locus_tag="ACHLCY_004060"
     CDS             complement(7383..8420)
                     /gene="curA"
                     /locus_tag="ACHLCY_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP0371212.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8601..9119
                     /gene="mddA"
                     /locus_tag="ACHLCY_004061"
     CDS             8601..9119
                     /gene="mddA"
                     /locus_tag="ACHLCY_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP0371213.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            9116..9565
                     /gene="ydcZ"
                     /locus_tag="ACHLCY_004062"
     CDS             9116..9565
                     /gene="ydcZ"
                     /locus_tag="ACHLCY_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0371214.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9566..9799)
                     /gene="ydcY"
                     /locus_tag="ACHLCY_004063"
     CDS             complement(9566..9799)
                     /gene="ydcY"
                     /locus_tag="ACHLCY_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP0371215.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9885..10058)
                     /gene="ortT"
                     /locus_tag="ACHLCY_004064"
     CDS             complement(9885..10058)
                     /gene="ortT"
                     /locus_tag="ACHLCY_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP0371216.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            10253..10348
                     /gene="yncL"
                     /locus_tag="ACHLCY_004065"
     CDS             10253..10348
                     /gene="yncL"
                     /locus_tag="ACHLCY_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP0371217.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10750..>11398)
                     /locus_tag="ACHLCY_004066"
     CDS             complement(10750..>11398)
                     /locus_tag="ACHLCY_004066"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP0371218.1"
                     /translation="DKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRF
                     CSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGN
                     DVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDEL
                     LRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT         2859 a         2729 c         2652 g         3158 t
ORIGIN      
        1 tttccaaagc actgcaccac cacctggtgc cggatgatct tgcttcagtg gtaagagtac
       61 ctgatgcatt gttcccaaaa tggtgttttc ccctgcccct ccaataatgt gtagctctca
      121 cttgataatc tattgattag gttatattaa gaatcaacat ttaaaataca tccattttct
      181 tgaataaatc aacatgttta ttaatcaatt gctgttttag ctctgactca tcaagctctc
      241 catatttgtc ctacagataa ttgcgaataa atacagagaa cgccatttat ccttttagca
      301 tacctgccgg atatttttat ttgtattaat attaattgca tctccaactc cgagcatatc
      361 catcattaaa aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac
      421 aatgtgaata gttagtttct tttcagagga aatgaaatag acaagctgaa tgaaggggag
      481 agtgatataa atcaaaaaac ataacatgaa tttaattaat tacacccatc attgtggata
      541 aagttactga ataataaata acataatctt ctggtatccc actcaaataa tttcattcct
      601 ttttaatact tcttgtaatt tcggtaactg acatacagca tcagcgatcg cgctgatatt
      661 ctgcgcatta tcctgaaacc attcatgccc cggcccccat gtgcgcatgg tgcagagata
      721 acagtccacc agcgttagtt gctcaccaaa ggcataaggt tcagcggtca attgtgaatt
      781 cagccagata taaagtgatt tgcgatattc aattacattc ttctttaact gttcaggtgc
      841 gtcagatgcc caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac
      901 cagccagacc aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag
      961 gtccgggcga cgatcgagaa ccataaatgc aatcgccgct gtctccgtca taatctcgtc
     1021 attttccagc gccagtgtcg gaacctgaca caacgggttc agggttttca ataactcgcg
     1081 tgaagctccc tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag
     1141 tgttagcatc agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat
     1201 atgccctcac aatcttttaa agattaagtg taggacagta cacaggttaa ccagcaaaaa
     1261 gatttgtcat tttttcgtca gttgtattat tccccttcag gattgtaagg ggatgttttg
     1321 catcgtttat cgataacggg taggatgctg cgccgatagt tttataccca taaagaataa
     1381 tggtgataac tatcatcgcc aggatgaata aacattgttc atggcaactt atatgacttt
     1441 ttcattaaag caatcaggga gagcaatgag taaacacgac accgacactt cagatcaaca
     1501 cgccgcgaaa cgccgctggc ttaatgccca cgaagagggg tatcacaaag cgatgggcaa
     1561 tcgccaggta cagatgatcg ccattggcgg cgcgattggc accggcttgt ttttaggtgc
     1621 aggagcccga ctgcaaatgg cgggtccagc attggcactg gtttatttaa tttgtggctt
     1681 gttttcgttt tttattctgc gtgcattggg tgagctggtg ctacaccgcc cttccagtgg
     1741 cagttttgtc tcttatgccc gtgagttttt gggtgagaaa gccgcttatg ttgctggctg
     1801 gatgtacttc atcaactggg cgatgacggg gattgttgat attaccgccg tcgctctgta
     1861 tatgcattac tggggtgcgt ttggcggcgt gccgcagtgg gtctttgcgc tcgctgcact
     1921 taccatcgtt ggcaccatga atatgatcgg tgtgaaatgg tttgcggaga tggagttctg
     1981 gtttgcgctt attaaagtgc tcgccattgt gacctttttg gtcgtgggta cagtgttcct
     2041 cggtagtggt cagccgctgg atggcaacac cactggcttt catttaatta ccgataatgg
     2101 cggcttcttc ccccacggtt tgctgcctgc tctggtgttg attcagggcg tagtgtttgc
     2161 ttttgcctcc attgaaatgg tgggtacagc tgccggagaa tgtaaagatc cgcagaccat
     2221 ggtgcctaag cgattaacag cgtgatttgg cgtattggcc tgttttacgt cggctccgtg
     2281 gtgttgctgg ttatgctatt gccgtggagc gcgtatcagg cggggcaaag tccgttcgtg
     2341 acgtttttct ctaaactggg tgtgccatat atcggcagca ttatgaacat tgtggtgctg
     2401 accgctgccc tctccagcct gaactcaggt ctgtactgca ccggacgtat tctgcgctca
     2461 atggcgatgg gcggttccgc accgagtttt atggcgaaaa tgagtcgtca gcatgtgccg
     2521 tatgccggga ttctggcgac actagttgtg tatgtcgtcg gcgtattcct caactatctg
     2581 gtgccgtcgc gcgtatttga gattgtgttg aacttcgcgt cgctgggaat catcgcttca
     2641 tgggcgttta tcatcgtgtg ccagatgcgc ctgcgtaaag caattaaaca aggcaaagca
     2701 gcggatgtca gttttaaact gcctggcgcg cccttcactt cctggctgac attactgttt
     2761 ttactgagtg tcctggtgct gatggcgttc gattacccga acgggactta tactatcgcg
     2821 gcgctgccga ttatcggtat tctgctggtt ataggctggt ttggtgtgcg caaacgcgtt
     2881 gctgaaattc acagcactgc gccagtcgtc gaggaagatg aagaaaaaca ggaaattgtg
     2941 tttaagcctg aaacggcgag ctaattgcaa aattgcccgg ctacgtgccg ggcaaattta
     3001 gattactgac gaaggtgtgc cgcatgcggc gtgataaaag tcaggcaagt ctctccgttt
     3061 tcaccctcgc cacatctatt acagcgcaat acgaatcaca tcgtccggct gggtagcttc
     3121 ctgctgttta gtggattttt gtttcacact gacatacagc gttttgccat cggcagacag
     3181 cgccaggctg tttggatgag tcggcgtatc gaacgttttc accactttat agcttttcgc
     3241 gtcaatcaca ctgactttac ctgcctgacg atgcgttacg taggcttcat tacgcgctgg
     3301 gttaaacagc acagccagtg attctggtgc cgcaaccttc gccagaatat tgccattacg
     3361 ggtatcgacc actaacactt ctgcggcttt agaatcggtg ataaatgcac gctgcctggc
     3421 ggtatcaagg ctaatgttga taaagaagtg ctctttgccg tcatccagca gctttttacg
     3481 gctgaggatt ttattgtcgg cggtgtcgat ggtaatcaat tcgccgtcag cgttagtggt
     3541 gtaaagacgt ttgcctttgc tatccagcgc cagaccggta ctcattttac cggtgttctg
     3601 gatggcggtt ttcagtttga tattctcgcc atcaacgacc caaatcacgc tctctttacc
     3661 aataccactg atgtaaacgg tgttcgtggc atcatcagct accagctcac gcggttgcag
     3721 cgggcgcacc tcttccgtgc gcttacgatc atccagcacc agacggcctt tcacctcgcc
     3781 cgttttggca tctatcgccg tgaccgcgct gtttacggtg ttaccaaacc acaacgtctg
     3841 agtcgtgtta ttgatggtgg caccaaacgg cttgagatcg ttatggatcg cctgcgtcac
     3901 ttccagagtg accggatcaa gacgataaac cacgccacct ttatccagtt tgcggctttg
     3961 cgaagtggcg agccacagtg cgttttcttg ctggctataa gccatttcgt aggcaccttt
     4021 acctaccgct ttacgcagca tttcttctgc ggcctgcgta ctgaatgatg aagcaacaag
     4081 caatgaacct aacagtaatg aaccacgcag gcgcgatgaa aacagatgac gtaaatgcat
     4141 gacgactccc tttgatgaac cgatattttt gctgcttcac aatgcttcgc taagcgtaat
     4201 catcgttacg ctcttgttat aaatgggaat ggtaatcatt attttctcaa atgtggagca
     4261 tttcttgccg ttacgactac aattttctca attacttaac gtatcgtgcc gtttcgtttt
     4321 ccataaattt acaatttcat taccataacg gcgttttccc attcttaacc tggctttttt
     4381 catgaagatt ttttccgtcc gacagaccgt tttgcccgca ctgcttgtcc tttcccccgt
     4441 tgtttttgcc gctgatgaac agactatgat tgtcagtacc gcaccgcagg tggtttcaga
     4501 actggatacc ccagcagcag taagcgtggt ggatggcgag gagatgcgcc tggcaacacc
     4561 gcgcattaac ttgtccgaat cactgaccgg cgtgcctggt ttgcaggtac aaaaccggca
     4621 gaactatgcg caagatttac agctgtcgat tcgcggattt ggctcccgct ccacttacgg
     4681 tattcgcggt attcgcctgt atgtggacgg tattcccgcc accatgcccg acgggcaagg
     4741 gcaaacatcc aacatcgatt taagcagtgt gcaaaatgtg gaagtgctgc gtggcccctt
     4801 ctctgccctg tatggcaacg cgtctggtgg ggtaatgaat gtcaccaccc agaccggaca
     4861 acagccacca accattgaag ccagtagtta ctacggcagt tttggcagct ggcgctatgg
     4921 gctgaaagca acgggcgcaa cgggagacgg cacacagcct ggcgatgtcg attacaccgt
     4981 ctcaaccacg cgttttacga cccacggcta tcgtgaccat agtggcgcac agaaaaattt
     5041 agctaatgcc aaactgggcg tacgcattga tgaagccagc aaattaagtc tgattttcaa
     5101 tagtgtggat atcaaagcag atgacccagg tgggctaacc aaagcagaat ggaaggctaa
     5161 tccacaacaa gcgcctcgtg cagaacagta cgacacgcga aaaaccatca agcaaactca
     5221 ggctgggttg cgctatgagc gtagcctgag cgcgcaagat gatatgagtg tgatgatgta
     5281 tgccggagag cgagaaacga cccagtacca gtcaatcccg atggcccccc aacttaaccc
     5341 gtcacatgcg ggcggcgtga ttaccctgca acgccattac caggggatag acagccgctg
     5401 gacacaccgt ggtgaactgg gcgttccggt cacgttcact accggcctga actacgaaaa
     5461 catgagtgaa aaccgcaagg gctacaataa cttccgcctg aatagcggca tgccggagta
     5521 cgggcaaaaa ggtgagttgc gtcgcgacga acgcaatctg atgtggaaca tcgatcccta
     5581 tttacagacg cagtggcagc tgagcgaaaa actgtcgctg gatgctggcg tgcgctacag
     5641 ctccgtgtgg tttgattcca acgaccatta cgttactccg ggtaacggcg atgacagcgg
     5701 tgatgccagt tatcataaat ggctacctgc cggttcgtta aaatatgcaa tgaccgatgc
     5761 ctggaatatc tatctggcag ccgggcgagg ttttgaaacg ccgacgatta atgagctgtc
     5821 ttatcgtgct gatgggcaaa gcggtatgaa ctttggttta aaaccatcca ccaacgatac
     5881 aattgagatc ggcagtaaaa cgcgtattgg tgatgggctg cttagtctcg cattgtttca
     5941 gaccgacact gatgatgaaa ttgttgtcga tagcagtagc ggtgggcgta cgacttacaa
     6001 aaatgccgga aagacccgtc gtcaaggcgc tgaactggca tgggatcaac gtttcgcagg
     6061 agattttcgc gtaaacgcgt cctggacctg gcttgatgcg acctatcgca gcaatgtttg
     6121 caatgaacag gattgtaacg gtaatcggat gccagggatc gcccgtaata tgggctttgc
     6181 gtcgataggt tatgtaccgg aagatggttg gtatgcaggc acggaagcgc gttatatggg
     6241 cgatattatg gcagatgatg aaaatacggc aaaagcgccg tcttatactc tcgtcggctt
     6301 attcaccggg tataaataca attaccacaa tttaactgtg gatttatttg gtcgtgtcga
     6361 taatttattc gataaagaat acgttggttc tgtcattgtc aatgagtcaa acgggcgata
     6421 ttacgaacct tcgcccggac gaaattatgg tgtcggcatg aatattgcgt ggagatttga
     6481 gtaattacct tgatgcccgg tatttgccgg gcatttactt taacgattgt attgctggta
     6541 taaaatagca atattttgtt gcataatttc agcaagacaa tgtctgcatc cctctttgtc
     6601 ttttgctttt aacgcagcga gtaacagatg atagttttct atatgctccc gtaattccgc
     6661 tggattaatc gcttcataga gataatgtaa accaggcccc atcctgaccc acagttgctc
     6721 aatcatctca cacaggatgg gcatatttga gcgatgataa atcgctaagc gaaatagcct
     6781 gtttacatta atgatttgtt ccatgtcgcc cttttcttgc gcctgctgta atttctcgag
     6841 caattcctgg agttccgcca ggtcttgcgg ggtgaggttt tcaacagcca gagcaactgc
     6901 cattaattcc agttcgtagc ggatccgatt gatttcgtct aattgacgtt tcctcacttc
     6961 cggaactgta aatgcttgtg caggtgcgac agaaagcgca ttcaccgaaa ccagacgtaa
     7021 taatgcttca cgcacaggtg taatactcat acctaattgc tccgccagat ttttagtaat
     7081 caggcgtgcg ccaggtttta atgcgccaat actaagctga tgtttcaggt cgttctcaac
     7141 ctgcagagtc aaactgacat gtttcatttt tcccattcca ggcattttaa ttccttcgcg
     7201 tgttttctgt ccaaccctgc ctgcacggca gaaggagttc acctggtgta ttaaagtgaa
     7261 taatcataaa ccacccacct ttgttgttat tagcagcaga acaatgacgt ggttagttaa
     7321 ttaaagctga aaataagctt tttgcctgga tttttatggc ggcttacgcc gccgttgaaa
     7381 ctttaatcat cacccgcgac gcggatcacc actttgccga agtttttacc cttcagcagg
     7441 ccgataaacg tctgtggcgc attctctaaa ccatcagtaa tatcttcgtg gtagtggatt
     7501 ttatcctctt tcacccattg ccccatctcc ttctgaaact catggatgcg gtgaccataa
     7561 tcctgagcga taataaaccc ttgcaagcga atacgttttt tcagcaccgt tgccatcaac
     7621 aggggtaaac gatccggacc gggtggtagc tctgtagcgt tatagctgct cactaagcca
     7681 caaacgggaa tgcgcgcaga tgtattaagt aatggtagca ctgcatcaaa taccttaccg
     7741 cccacgtttt cataatagat atcaatacct tttgggcacg ctttcaccag ttgttcggca
     7801 aaatcatccg cgtggtgatc aagacaaaca tcgaagccca gcacctcgat agcatggcgg
     7861 catttttccg cgccaccggc aacccccacc accctgcaac ctttaagttt gccgatttgc
     7921 cccaccgttg ctcccacagg acctgtcgcc gcagctacca ccaacgtttc gccctcttta
     7981 ggctgaccga tatccagcag ccccatataa gcggtaaagc ctggcatccc cagcacaccc
     8041 agcgaccacg atggattttg cggatgatcg ccaagtttca ccagatcatc accactggat
     8101 atgtcatagt cctgccatcc actgtagccc agcacccagt cgccaggctg ataatcagga
     8161 tgattcgact ccacaacacg gctcaccgtg ccgccgacca tcacgccgcc aatatcaaca
     8221 ggcggtgaat aagatggctc atcgctcata cgtccacgca tatacgggtc cagggacaaa
     8281 taaactgtgc gcagtaacac ctgtccttca cccggtgtgg cgacatcatc ttcttcaaga
     8341 cggaaattct ccggaaccgg tgcgccatgt ggacgcgagg ccagaaccca acgtcgatta
     8401 cgctgctttt gttgccccat aatgctctcc tgtgcttttg gcatgaatcc atcaatagta
     8461 gcaggctcac gggattggtt ttgtttgcag ccgtcacatc ccctctgcaa cacgcaataa
     8521 tttcatcgag gaatccgttg cttcgggctg ttttcgatag tattgataac atcatcatca
     8581 ttattttatc gaggccagac atgtccatcc gttttgcccg caaagccgat tgtgctgcca
     8641 ttgcggaaat ttataaccac gccgtgttgt atacggcggc tatctggaat gaccaaacgg
     8701 ttgatgcaga taaccgcatt gcctggtttg aagcgcggac catagcaggt tatccggtgc
     8761 tggtgagtga agaagatggc gtagtgacgg gatatgcctc gtttggcgac tggcgtagtt
     8821 tcgatggttt tcgccatacc gtggaacatt cggtttatgt ccatcccgat catcagggca
     8881 aaggtctggg gcgtaaattg ttaagccgat tgattgatga agcgcgggat tgcgggaagc
     8941 atgtcatggt cgccgggatc gaatcgcaaa atcaggcctc gctgcatctg caccagtcgc
     9001 tgggatttgt cgtcaccgca caaatgccgc aggtgggcac taaatttggg cgctggctgg
     9061 atctgacatt tatgcagttg caactcgacg aacgcactga accggacgcg attggatgaa
     9121 tcagtcgctc acccttgctt ttttgattgc cgccggaatt ggtctggtag tccagaacac
     9181 gctgatggtg cgcatcaccc agacctcctc taccattctc atcgccatgt tgctgaactc
     9241 actggtgggg attgtgctct ttgtttctat tttatggttt aaacaaggca tggcggggtt
     9301 tggcgaactg gtgtccagcg tgcgctggtg gacacttata cccggcttgc tgggatcgtt
     9361 ttttgtcttc gccagtatca gcggatatca gaatgtggga gcggcgacaa ctattgcagt
     9421 actggtcgcc agccagctaa ttggcggtct gatgctggat atcttccgca gtcatggcgt
     9481 accactacgg gcgttgttcg gcccaatttg tggcgcaatt ttacttgtgg ttggcgcctg
     9541 gttggtagcc agacgctcat tttaatcaga ggatggtgcc gcctttggtt aactgttcgt
     9601 ggcgcgcttc catatcttct ttatgcttgc gaccgtgatg agcaatagcg gtgcgcaaac
     9661 gctgctgctg ggtgtagcga tcttcccgac ttaactccgc gtcatcgctc agggcgatta
     9721 ataattcgtt catatgggca atgacgctct cttcaatcgc ggcatccacg cgcgcgatga
     9781 cttcatccag atgcgacata ctttctcctt aaccacttgt gaaatttatc gattcatcca
     9841 aatgatataa gtctgcgtgg tgccgtcaag ccagtgagta agatttaaaa cagtgaaaac
     9901 aacagcgcca ccgggaaact catcggccat gttgccccca ccagaaaggc gcttaagaaa
     9961 cggatgcgtt tcttatcgtg agaaagaaac caggtgataa gaaatgcgat tgctgccata
    10021 accgcataaa aaacaagcat atgttgatag agagacatgt acgggcgtcc agccagatga
    10081 ataaaacgcg cgcaaatatg cgtcattttt tgacgtgcat caagtttttt caccagtttg
    10141 agaaaaaatc atcaaacagc aacttgccct tatccatctt cagaatgagt attattgcgg
    10201 attttcttaa cccgtactat acacataggg gcaatgataa aaggtggcaa aaatgaatgt
    10261 ttccagtaga actgtagtac tgataaatgt ctttgctgct gttggtttgt ttactcttat
    10321 ctctatgaga tttggctggt ttatttgatg tagtgatttt gcacgcagca cgtactgtca
    10381 gagaaatcaa ctctctgaca gtacgcaaga tcttctcata ctctccaggt ttacaacatt
    10441 atccttaata tgtacttctt tttgctggca tctacattga aatgagtaga aaacatcccc
    10501 ctactgtcgt ctatacaaac agcttactga ttcaaacgga atcttgtttt cacatttttc
    10561 tatcatgaat actttatacg cgcgggagtc tataaatgct ttatactttc aaggaataac
    10621 gtaaatataa aaagatggcg taaagtatat gcatggcagg atgtttgcaa cctccttgat
    10681 gacgctgccc cattgaaatt gcaaaccccg ccctggttat cttgcccatt tatcatgcaa
    10741 aacagataat tataggcctc tgaatacctc agaaagattg aattttttat agtccctctc
    10801 ttgaatattt ccatctgata tcaatcgatt catctcttca acaaaataat ttatatcatc
    10861 acacttgtta ttatccggaa gatctgagta gttataattc caccatttta ttctcaacag
    10921 ttcatcaata acatttgaat caaacctaaa tctgattatt tttgcaggaa ctcctgcaac
    10981 tatggcatat gggggcacat ctttggtaac aacggaattg gcagcaataa cagcaccatc
    11041 acctattgcg atgccacctt tcaatacaac atcatttcct atccagacat catttcctat
    11101 aatcggattt tcaggtttgg taatgaatgg tttcaatgtc cagctacgtt caaattcact
    11161 catagcaaac ttatcaaatt caccgttata agtcatcatg tgagtagtaa aacgattcaa
    11221 aggatgattt ccacccatta tttttacatt tgatgcaata ctacaaaatc gacctatacg
    11281 tatattccct gaaaaatggc aggtcggaaa tgaaaaagcg ccgacactgg agaaattttt
    11341 cacaggcatt gttgaatatt cttcagcaat aacattatct tcaattgtta tcttgtca
//
LOCUS       DAZGTR010000137        11258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGTR010000137 DAZGTR010000000
VERSION     DAZGTR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.139"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..266
                     /locus_tag="ACHLCY_004067"
     CDS             48..266
                     /locus_tag="ACHLCY_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0371219.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTSSLLKRIRNGKKAKS"
     gene            244..1314
                     /locus_tag="ACHLCY_004068"
     CDS             244..1314
                     /locus_tag="ACHLCY_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0371220.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELLSGNRRESLIDRIKGADAITLHAWLDRYETILSERGIRPKTLLDYAS
                     KIRAIRRKLPDKPLADISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEGHV
                     ATNPVTATRTAKSEVRRSRLTANEYVAIYHAAEPLPIWLRLAMDLAVVTGQRVGDLCR
                     MKWSDINDNHLHIEQGKTGAKLAIPLTLTIDALNISLADTLQKCREASGSETIIASTH
                     HEPLSPKTVSKYFTKARNASGLSFDGDPPTFHELRSLSARLYRNQIGDKFAQRLLGHK
                     SDSMAARYRDSRGREWDKIEIS"
     gene            complement(1309..1935)
                     /locus_tag="ACHLCY_004069"
     CDS             complement(1309..1935)
                     /locus_tag="ACHLCY_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HFP0371221.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKLYIKNFINYQYVNVRWSLLIKIKPL"
     gene            complement(1932..3620)
                     /gene="yfaA"
                     /locus_tag="ACHLCY_004070"
     CDS             complement(1932..3620)
                     /gene="yfaA"
                     /locus_tag="ACHLCY_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP0371222.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(3769..6396)
                     /gene="gyrA"
                     /locus_tag="ACHLCY_004071"
     CDS             complement(3769..6396)
                     /gene="gyrA"
                     /locus_tag="ACHLCY_004071"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP0371223.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6543..7265
                     /gene="ubiG"
                     /locus_tag="ACHLCY_004072"
     CDS             6543..7265
                     /gene="ubiG"
                     /locus_tag="ACHLCY_004072"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP0371224.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            complement(7393..11127)
                     /gene="yfaL"
                     /locus_tag="ACHLCY_004073"
     CDS             complement(7393..11127)
                     /gene="yfaL"
                     /locus_tag="ACHLCY_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP0371225.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGVVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAASGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNANVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
BASE COUNT         2723 a         3046 c         2735 g         2754 t
ORIGIN      
        1 gccgcatcaa tgcggctttt cttgcgtgta attgcggaga ctttgcgatg tacttgacac
       61 ttcaggagtg gaatgcacgc cagcgacgcc caagaagcct tgaaacagtt cgtcgatggg
      121 tgcgcgaatg caggatattc cctcctccgg ttaaggatgg aagagagtat ctgttccacg
      181 aatcagcggt aaaggttgac ttaaatcgac cagtaacaag tagccttttg aagaggatca
      241 gaaatgggaa gaaggcgaag tcatgagcgc cgggatttac cccctaacct ttatataaga
      301 aacaatggat attactgcta cagggaccca aggacgggta aagagttcgg attaggccga
      361 gacaggagga tagcaatcac tgaagcaata caggccaata ttgagttgct atccgggaac
      421 aggcgtgagt cactgataga cagaattaaa ggcgctgacg caatcactct tcatgcgtgg
      481 cttgaccgat atgaaacaat cctcagcgag aggggtatca ggccgaaaac tctactcgac
      541 tacgccagca aaatcagggc aatccgaaga aaattgccgg acaaaccgct cgctgacata
      601 tcaacgaaag aggtggcagc aatgctaaac acctacgtag cagaaggtaa agcggcttcc
      661 gcaaaattaa tcaggtcaac ccttgttgac gtttttcgtg aggcaatagc cgaggggcat
      721 gtggctacga atccggtaac agcaacccgc acagcaaaat cagaagtaag gcgttcaaga
      781 ctgacagcta atgaatatgt cgcgatttac catgcagccg aaccgctccc aatctggctg
      841 aggctggcaa tggatttggc tgtcgttaca gggcagagag taggcgattt atgcagaatg
      901 aaatggtcag acataaacga caaccatctt cacattgagc agggtaaaac aggggctaag
      961 ctcgccattc cgctgacgct aacgattgac gcgctcaata tctcattggc tgatacacta
     1021 cagaaatgca gggaggccag cggcagtgaa acaataattg catcaacgca tcacgaaccg
     1081 ctttccccga aaacagtatc taagtatttt acaaaggcga gaaatgcatc tggactctca
     1141 tttgatggag acccgccaac attccatgaa ctgcgcagcc tgtcggcgag gctataccgg
     1201 aatcagattg gcgataagtt tgctcaacgt cttctcgggc ataaatcaga ttcaatggcg
     1261 gcgcgttaca gggacagtcg cgggcgggag tgggacaaaa ttgaaatatc ataatggttt
     1321 tattttgatt aataatgacc agcgcacatt aacatattga taattaatga aatttttaat
     1381 atacaacttc catgtcataa gttgttgcag actgactgcg cgcattccgt tgtcagtttt
     1441 cgtggcgctt ccggtgtggt agatgacgta acgccccatc cagaccatta agtgctgggc
     1501 atcgccctga tcgaaaaaaa tcatatcgcc aggcagcgcc tggtttatgt cctggccaat
     1561 aaactggctg ttgtactgaa tcaaattaat cgcggtcacg taggggccgg ttttcccgtt
     1621 cccctgattc cagttttgcg ccagttgacg ctgtccaggt gttagcgtca tctctggcgg
     1681 caaatactgg ctagagaaac cgttactttt tagccattta ctatcgtgaa ctttcagcgt
     1741 ctcgttcgcc gcaaatcgca ccaggcccgc acaatcctgc tgataccagc gtggacttgg
     1801 cccctggcgg agctgttctt gtgcaatgcg tacaaaccag gcgcgaaaca gcccggattg
     1861 ttcgacattc agcatttcgc tatgggcaac aacacaacac agccagcaaa tcagcgccag
     1921 cagcccgtgc ctcatagcgg ctgccaggtt atgggtagcc actgccaggc ggcaccgggt
     1981 tccatctggg ccagcttcat gacataacgc ggttgttgag ataaagcgtc cagcttcggc
     2041 attaataaag tttgtgcggc gttataaaaa accggttcga gattcttcgg cagactggtc
     2101 aacgtttcgt tacgcagcag tttcgccacg ccttgtggat tgatatagag cggaacgatg
     2161 ccatcagtgg gtattacatc caccattgcc gggcggtttt tattcagtgt ttgcagggcg
     2221 ttattaacca gcgtgtcatc gagggagaaa agcagcgttt tgttttgcat cgccagcgac
     2281 acgcggaaaa aataatccga cattaattga tcgggttgcg ccgcctgcgc tttcggatac
     2341 tgaccgtatc gggaactcac ttcgcgacgc cagatttgcg cttcgccctg ctgagtctgg
     2401 cttatcggca aaacaccttc tggcgctttg ctttcgtgcg caccaatatt ttgcgtaaac
     2461 agtttccctg gcaattgcgc ctgttcggca gtgccatcaa actgaccgac aaacagcggg
     2521 gtttgcaatt ttgagtctgc ataccagcac agccccgcgg caccgtctaa cgccccattc
     2581 aacttgtcgt tttcctgact gatgtgcgaa agcatctctt cggcaatacc gtgtgaatac
     2641 ggcaccacca cacagaagct ggccccggca ggcatactgt tccagaccgg cgtaaaatcg
     2701 aaactggtat ctacgctggc ggattcatca tttaacgcca caaaactgtg ccagccgtcg
     2761 ttacccattt cgaagcgtac gccagcaaaa gaaggcatta accgctggta gccaaaaccc
     2821 agcaacctgg cgctgaccac gatgcgctgg cgtactggcg ttttttcagc agcacgctct
     2881 tccaggccaa agcttgcttg ccagcgtttt ttgccgctca acaaatcacc tgcgatcgcc
     2941 gtggcttcgg tatcctgctg atcatcttta aacaacatat ccgtgctgga aaacaccagc
     3001 atcttgtcct gataagtcgc gaacatcagg gcgttattgc cgttatagcg caactgataa
     3061 acaggaacag tttcactatt tatcttgatg ctactgattt ccgttttgct taactggcta
     3121 tcgctggtag cggcaaacaa caatggctcc agcagtttgc ttaaaccact gcgctggatc
     3181 agcaccatat aatgtgaaag atggccttgt ttatcgtgcc acagcgctgc ctgcgcgggc
     3241 tgatctaaga gtgacgaaaa gagcttatct ttcagcgtga gatcgtgttc atagacaata
     3301 cgacgaatgc tgccttcaat gcccagacga tcggcatgat tctgataata gaaaacgaaa
     3361 tcttcgctca gaacatcgtg gagaaacgga atggtgagga gatctttggg aagctggctc
     3421 agagagtcgc tgtcgagaaa gaggtccggc tcattgagat cgatttgcag attgttgtgc
     3481 accaccagcg gcgacaacgt tttttctggc ccactgccag catattgcaa cgcccagacg
     3541 ccagcggaaa gcagtgctat tgcgccaaaa cctacaagac catagaaccg ccagcctttc
     3601 gccttttttt caccactcat tgccacattc cttgtgtata gccagccatt ttttacgggc
     3661 acagccaaac tttaccgtgc cctaatacga caaaagccca gactttgcag cctggacttt
     3721 tcaattcaaa caagggagat agctcccttt tggcatgaag aagtaaaatt attcttcttc
     3781 tggctcgtcg tcaacgtcca cttccggagc gatttcatcg tccccttccg cggcactgcc
     3841 atcgatggta tccagatctt cctcgtcaac cggttcagca acacgttgca gacccactac
     3901 gttttcatct tccgcagtac ggatgaggat cacgccctgg gtgttacggc ctacgatgct
     3961 gatttccgaa acgcgagtac gtaccagcgt accggcatcg gtgatcatca tgatctggtc
     4021 gcagtcatct acctgtaccg cgccaaccac taaaccatta cgttcagtaa ccttaatgga
     4081 gataacccct ttcgtcgcac gcgacttggt tgggtattcc gccactgcgg tacgtttacc
     4141 gtaaccgttt tgcgttgcgg tgaggattgc gccatcgcca cgaggcacga tcagagagac
     4201 gactttatcg ccttcaccta agcgaatacc gcgaacaccg gtggtgttgc agcccatcgc
     4261 acggacagaa gactctttaa agcgcaccac tttaccttca gcggagaaca gcattacttc
     4321 gtcttcgccg ctggtcaggt caacgccgat cagctcatcg ccgtcaacca gtttgatcgc
     4381 cactttaccg gcggtacgca gacggttgaa ctcggtgagg acagttttct tcacggtacc
     4441 gttagcggtc gccatgaaga ctttcacgcc ttcttcaaac tcggtcactg gcaggatcgc
     4501 agtgatacgt tcgtcctgct ccagcggcag caggttgacg atcggacgac cgcgcgcgcc
     4561 acgagtggct tccggcaact gataaacttt catcgaatag acgcgaccac ggctggagaa
     4621 gcacagaata tggtcgtgag tgttcgccac cagcagtcgg tcgataaagt cttcttcttt
     4681 aatacgtgcg gcagatttac ctttcccgcc acgacgctgc gcttcgtatt cagaaagcgg
     4741 ctgatactta acgtagccct ggtgagagag cgtcactacc acatcttcct gagtgatcag
     4801 atcttccagg ttgatgtctg cgctgttggc ggtgatttca gtacgacgtt tgtcaccgaa
     4861 ctgttcacga accagctcca gctcttcacg gatcacttcc atcaaacgat cggcgctacc
     4921 aagaatacgc aacagttccg cgatctgatc cagcagctct ttgtattcgt cgagcagttt
     4981 ttcgtgctca aggccggtca gtttctgcaa acgcagatcc agaatcgcct gagcttgctg
     5041 ttcggtcagg tagtacagac catcacgcac gccgaactct ggctccagcc attccggacg
     5101 cgcagcatcg tcgccagcac gttcgagcat cgcggcaacg ttgcccagct gccacggatt
     5161 agcaaccagc gcagttttcg cttctgcagg cgtcggcgca tgacggatca gttcgatgat
     5221 cgggtcgatg ttcgccagcg ccacggctaa tgcttcaagg atatgagcac gatcgcgagc
     5281 tttacgcagt tcgaaaatag tacgacgggt caccacttca cggcggtgac gaacaaacgc
     5341 cgcgatgatg tctttcaggt tcatgatctt cggctgacca tggtgcaatg ccaccatgtt
     5401 gataccgaaa gaaacctgca actgggtctg ggagtagagg ttgttgagca caacttcacc
     5461 gaccgcatcg cgtttcactt caatcacgat gcgcataccg tctttgtcag actcgtcacg
     5521 cagcgcgctg atgccttcca cgcgtttttc ttttaccagt tccgcaatct tctcgatcag
     5581 gcgcgctttg tttacctgat acggaatttc gtggacgata atggtttcac gaccggtttt
     5641 ggcgtcaact tccacttctg cgcgagcgcg gatatacacc ttgccgcgac cggtacggta
     5701 agcttcttca ataccgcgac gaccgttaat gattgccgcc gtcgggaagt ccggccccgg
     5761 gatgtgttcc atcagccctt caatgctgat gtcttcatca tcgatatacg ccagacaacc
     5821 gttgatgact tccgtcaggt tgtgcggcgg gatgttggtt gccataccta cggcgatacc
     5881 ggaagaaccg ttcaccagca ggttaggaat tttggttggc atgacgtcgg gaattttttc
     5941 cgtgccgtca tagttatcaa cgaaatcgac cgtctctttt tcgagatcgg ccatcagttc
     6001 atgggcaatt ttcgccagac ggatttccgt ataacgcatt gccgccgcag agtcgccgtc
     6061 gatggaaccg aagttaccct gaccgtctac cagcatgtaa cgcagcgaga atggctgcgc
     6121 catacggacg atcgtgtcat aaaccgccga gtcaccatgg ggatggtatt taccgattac
     6181 gtcaccaacg acacgggcag attttttata ggctttgttc cagtcattgc ctagtacgtt
     6241 catggcgtaa agtacgcgac ggtgtaccgg cttcaggcca tctcggacat ctggcagcgc
     6301 acggccaaca atgaccgaca tcgcataatc cagataggag ctcttcagct cttcctcaat
     6361 gttgaccggt gtaatttctc tcgcaaggtc gctcatctaa ccgctatccc tctactgtat
     6421 cccggattca aaggtcgcaa attataacac agccgcgcag tttgaggtaa acctatacgc
     6481 tttattcaca tccaatgcct gatatactcg tttgtcttgc caattacgga gtagaagtgc
     6541 caatgaatgc cgaaaaatcg ccggaaaacc ataacgtaga ccacgaagag atcgctaaat
     6601 ttgaagccgt cgcctcccgc tggtgggatc ttgaaggtga gttcaaaccg ttgcaccgca
     6661 ttaacccgct gcgtctgggc tatattgccg agcgtgctgg cggtttattt ggcaaaaagg
     6721 tgctcgatgt cggttgtggc ggcggcattc tggccgagag tatggcgcgc gaaggcgcga
     6781 cggtgaccgg tctggatatg ggctttgagc cattgcaggt ggcaaaactg cacgcgctgg
     6841 aaagcggcat tcaggtggat tatgtgcagg aaaccgtgga agagcacgcg gcaaaacatg
     6901 ccgggcagta tgatgtggtg acctgcatgg agatgctgga gcacgtcccc gatccgcagt
     6961 cagtggtcag agcctgtgcg caactggtga aaccaggcgg cgatgtcttt ttctcgacgc
     7021 ttaaccgcaa cggcaagtca tggctgatgg cggtggttgg cgcggaatat attttgcgca
     7081 tggtgcccaa aggcacgcat gatgtgaaga agtttattaa accggcagaa ctgcttggct
     7141 gggtagatca gacaagcctt aaagagcgac atataaccgg gctacattac aacccgctca
     7201 ctaatacttt taaactcggc cccggcgtgg atgtgaacta tatgctgcac acgcagaata
     7261 agtgaggttg ttgtttggcc gagccaatgc ctgatgcgac gcttgccgcg tcttatcagg
     7321 cctacaaatg ctccccgtag gccggataag gcgtttacgc cgcatccggc aaccgtgccg
     7381 actagacagt gattaccatt tcaccgtcat cgacaaaaac cctgccgtct gggcaaaatc
     7441 atcactccct ttctgccacg ccacgctgcc gcgcagggac actcgctgac tgatattgcc
     7501 cgtgactccc acttttattt caccccgtcg cttcaccgca tcgtcactga tagtgctgcc
     7561 atcttcttca atttccgtcg catggggatc gtgataataa ttcagatcta atgttggtgt
     7621 gacaccaacc gcggtacgcc attcgctgtg taaacccagc cgcgtctgaa tatcatcccc
     7681 ctgcgattgt gacacgcgcg catggttagc ggcggtaaaa tcatcctgct gcacgccctg
     7741 ataaatcacc tgcgcctgcg gttcaatcac cacaccacgc cccggtaacc actgataccc
     7801 ggcttccagc gaggcgataa tccccgacga gtggtaatga tctgtgccat cgtcttgttc
     7861 ggaaacatca ttgttaaacc acgcgtattg cagccagcta tccagccagg ccccctgctt
     7921 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     7981 atcggcgcga gttccggtca tattcgagcg gctctcgccc tgattatcac tgtagccacc
     8041 aaccgcccca agcatccact cgccatcatc gccccagcgc ccgctaaaca ggtcaccgct
     8101 aagctgcacc gtagaagtgt cttcatgctg agccagttgc cctgccgccg tgtaatgata
     8161 acgtccgccg ataacacgta aattcagcgt ctgaccattg ccacctgcat gatcgcgtcg
     8221 ctccatcata aacgcctgat ttgccgcccg cagattattg agataaccgc caacgttggc
     8281 attcagcacc ggctggtaag cggggattgg atcgggtgtt gggtcaggat cgggtgttgg
     8341 gtcagggtct ggatcaggtg gcaatggtgg cgttacttct tgcgatcgca gataccagtc
     8401 gttgttatct tccaccagcg tgtagtcata tgctcccata ttgacgtagc cgctgcctgc
     8461 cagactgaac ttcgcattgt tttgaaactg cgtgggatcg gctgcgaaat caaccacttt
     8521 aatgcctgtc gatgtcggct caccaatccc tgtaatggag ttaaccacca cagttgtgtt
     8581 gccagcagta ttaccgttca acaccaattg atcgcttacc gagtcatcgc cgtttaattc
     8641 gctatcgagc agtagcgtac cgccgccggt ataatcaccg tttaccgtca gcgtatcgcc
     8701 agcgacgcca ttttgcaggc taaccgcacc ggacgtattg gtcagattgc cattaaccgt
     8761 cacgtcggaa agataggtca ggctgtcagt ttcaaggttg gtattaagct cacctgtcaa
     8821 cgtcacgcca tcggtggcat tcaccagcgt cccatcgccg ttaaacagtg aggcattaag
     8881 ggcgacagaa gtatcctgcc cggtcaggcg caaaaccgta ccatcgctga tgtcgatagt
     8941 gccgctggaa gtagcatcaa tagattgaat atcctgatcc gcgccagtaa cgaacgtcgc
     9001 cccatcaccg acccacagtg acgaagcata aggaaggata tccgcgacat cgcctttcag
     9061 ggtgccttct tcgacacgta ccgccgactg cgttgtaccg ctggcggtca gctccagcgt
     9121 acccgccccc gttttagtca atgtgctacc ctcatcctga tgctgcccgc tgctgtcagc
     9181 catcagtgcg ccccactgcg tgtctacccc cgcatcaact gccacttcac cgtcggcgcg
     9241 catttcaata tcacgcccat gctcagtcgt cgtagagcga gtatcgacat tggcagtaat
     9301 ctccatcacg ctttcttgtt gcttatcggt aaagataacc tggcggttat agtgggtatc
     9361 tccaagctgt gagttgtcgc tcacctccag cataccggag gcgatttgtg ttgtgcccaa
     9421 ataactatta ttgtttgcca gacttacctg cccgtcgccc gcatcgacca ccacgtagac
     9481 gccatctttc ccgtcaccgc cgatatgcat atcgtttcca cctgcaagat ctaccacatc
     9541 ctgactgccg ataaccgttc cgctgctgcc gctgacttca aggccatcgt tgtatgatgt
     9601 gccactctgc caggtggaga aatcagagag atcgagcaca ccgccgttta acacgatcga
     9661 ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg ccttccagcg aaagcaccgc
     9721 accatcatca acgactatat cgccggttag cgccatcgac tgcgcccccg acagcacgta
     9781 gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg atggtgccag caaaactgcc
     9841 ttgattaaca gtaacattgc caccagcatc gatatttaaa gtgccattct gaaagcccgt
     9901 caatgagtgc acaaaggttt gttgggtgga gccaacgttt agctctgcct gattctggta
     9961 cttatcaata ctccctatcg tcagaccgta gcagtcttgc ggatcgtcct ggcaatgcgt
    10021 atcgccgaca ttcatcaggg agttgctgcg acccagggta acttcaccgt tttcaatctg
    10081 catctcacca gtaaagtcat tgttatctgc attaagtacc agatcgccgg aacctgtttt
    10141 ggtgatcaac ccggtaccag caatagagtc aacagctccg tcattctctg tattgccaat
    10201 aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca cttaagccga gatacataaa
    10261 gccactcgcc gcagtggaag gaccatctcc atagcctgct gcgctgttgt tctcatccac
    10321 taacacgccg ccgttctggc tgtagctgtc atcaacagaa atatcaataa gatagggagc
    10381 cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg gcagtgttat ttgtaaaggc
    10441 ggtgttattt attatcgtat aacctgaagg atgcttgctg tcgctattat tatcggtaac
    10501 atcgattgcc ccgccatcgc catcactgta acttgttgat gtatatgcct ggttgttatc
    10561 aaaaataaca tcacttaaat aaacgtcatt attaatggta taaattgcgc cacctttgcc
    10621 atcattagcg atgttattgc gaaacatggc gttagtgaca cgtaaatcga cgacaccagt
    10681 atcattagta ccagaagaat agattgcgcc accatagccg cctgcgacgt taccagaaaa
    10741 aataacatca gtaagattca gcgttgagtt ttctttagca aatatggccc cgccgttatt
    10801 atattctcct gtaacggtgt tattggcaaa cagagtcatt ccagtttcat tttctggtaa
    10861 tagtgaaaat tccgctcctt gttgcaaaaa tacggctcct ccgctggcgt tattggtcat
    10921 atccgaaaaa accagccatt gcccatcggc gacactccag tcctgcgtaa tgcctgaaag
    10981 gctttgcctg ctggcgtgac aactcgcttt gatatcatat ccctggcata aatcaatggc
    11041 cgccgcgaaa ccgttagcag agaaaagaga tgcaatcatt gacgggagta gagataaata
    11101 ctccttgcgt agaaagataa tccgcatatt catctaacca tcattttata taagacggca
    11161 tatttaaacg cattatacaa tatgggttat ctttcttttg taaattgtcc aacccgatgc
    11221 aatggctatt ttgttattat ttgatgaata gtatcagt
//
LOCUS       DAZGTR010000138        10878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGTR010000138 DAZGTR010000000
VERSION     DAZGTR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.140"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            72..800
                     /gene="gadW"
                     /locus_tag="ACHLCY_004074"
     CDS             72..800
                     /gene="gadW"
                     /locus_tag="ACHLCY_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP0371226.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1163..4276)
                     /gene="mdtF"
                     /locus_tag="ACHLCY_004075"
     CDS             complement(1163..4276)
                     /gene="mdtF"
                     /locus_tag="ACHLCY_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP0371227.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4301..5410)
                     /gene="mdtE"
                     /locus_tag="ACHLCY_004076"
     CDS             complement(4301..5410)
                     /gene="mdtE"
                     /locus_tag="ACHLCY_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP0371228.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5518..5796
                     /locus_tag="ACHLCY_004077"
     CDS             5518..5796
                     /locus_tag="ACHLCY_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371229.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5797..6324)
                     /gene="gadE"
                     /locus_tag="ACHLCY_004078"
     CDS             complement(5797..6324)
                     /gene="gadE"
                     /locus_tag="ACHLCY_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0371230.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7123..7695)
                     /gene="hdeD"
                     /locus_tag="ACHLCY_004079"
     CDS             complement(7123..7695)
                     /gene="hdeD"
                     /locus_tag="ACHLCY_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP0371231.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            7950..8282
                     /gene="hdeA"
                     /locus_tag="ACHLCY_004080"
     CDS             7950..8282
                     /gene="hdeA"
                     /locus_tag="ACHLCY_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP0371232.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            8398..8724
                     /gene="hdeB"
                     /locus_tag="ACHLCY_004081"
     CDS             8398..8724
                     /gene="hdeB"
                     /locus_tag="ACHLCY_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP0371233.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8788..9435
                     /gene="yhiD"
                     /locus_tag="ACHLCY_004082"
     CDS             8788..9435
                     /gene="yhiD"
                     /locus_tag="ACHLCY_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP0371234.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9477..10007)
                     /gene="dctR"
                     /locus_tag="ACHLCY_004083"
     CDS             complement(9477..10007)
                     /gene="dctR"
                     /locus_tag="ACHLCY_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0371235.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(10163..10729)
                     /gene="slp"
                     /locus_tag="ACHLCY_004084"
     CDS             complement(10163..10729)
                     /gene="slp"
                     /locus_tag="ACHLCY_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP0371236.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         2970 a         2476 c         2506 g         2926 t
ORIGIN      
        1 aaacagtctg gcaacatttc attagtatac tgaaattgaa ataatcgcag tatgaaatat
       61 aagggataat catgactcat gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt
      121 atcatgaaca gcataaaata tcgctgcata acgagagtat cgtgctgctg gagaaaaatt
      181 tggcagacga ttttgcgttt tgttcaccgg atacgcgacg actggatatc gatgagctga
      241 cagtttgcca ttacttacaa aatattcgtc agctaccacg caatttaggg ttacatagca
      301 aagaccgtct attaattaac cagtcacccc ccatgccgct ggtgacggcg atttttgata
      361 gcttcaatga atccggggta aattcaccga tactgagcaa tatgctctac ctttcctgtt
      421 tatcgatgtt ttctcataag aaagaactga tccccttact tttcaatagc atcagcactg
      481 tttcaggaaa agttgaacgc cttattagct ttgatatcgc caaacgttgg tatctgcgcg
      541 atatcgcgga aagaatgtat accagcgaga gtctcatcaa aaaaaagttg caggatgaaa
      601 atacctgttt cagtaaaata ttactcgctt ccaggatgtc gatggccaga cgattactcg
      661 agttacgtca aattcctctg catactattg cagaaaaatg tggctatagc agtacgtcgt
      721 actttataaa cacatttcga caatattatg gtgtaacgcc acatcagttt gcgcaacatt
      781 cgccaggtac cttttcctga catattttgc atttgaatat tggtcaggat ctcacacctg
      841 cttcatgtga aactcttccc tgatgatttc tgccgggcta ccggctagtt ctctttcgca
      901 gtttgccagg cggtataggt attttcagga ggaaaagact cggcatgttt gggattatta
      961 agctgacaat tcatacgatt acgggaatgt gggtcagtat tgtgctgttc aaattgatga
     1021 cgaatggctg gagtggtttc tactttcagt gctgtgtact aagcctggta ttcctgacgg
     1081 ttagctggtt gttgagtggt gaatggttag caggaaagag taaggctgaa ccttcacgtt
     1141 caaccttact ctcatttaca cgttacgctt ttttaaagcg ggcaaagaga tgttccacta
     1201 caacgaaaaa gaccggaacg aagtaaattg ccagcactgt tgcggcaaac atcccgccca
     1261 tcacgccggt acctaccgcg ttttgcgcgc cagaaccggc accatgactg ataaccagcg
     1321 gcagcacgcc gagaataaag gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg
     1381 ccgcttcgat gattgcctct atcggcgttt tcccttcttt ctgcatcatc tcaacggcaa
     1441 attcgacaat caggatggcg ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct
     1501 ggaagtagac gtcattactt aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc
     1561 ctaacggaac aaccaacatc accgagaacg gaattgacca gctctcatag agtgcggcga
     1621 gggcgaggaa caccacgacc agtgaaatgg catacagtgc aggagcctgg tttgaggata
     1681 acgcttcctg atacgacagt ccggtccatg agtagccgac gcctgccgga agtttagcga
     1741 ccaggtctgc cataaatttc atagcgtcac cggtactttt cccggccgcc gcttcaccta
     1801 aaatctccat tgacgggatg ccgttgtagc gttccaatcg cggtgaacca taggtccatt
     1861 cggtagacga gtaggcagaa agcggtgcca tcgtgccaga ggcgttgcgt acataccatt
     1921 ggttgatgtt atccggcaac atacggaacg gcgtgcctgc ctggacatac acttttttca
     1981 cccgcccctg gttgaggaag tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct
     2041 ggttgatatc agacagcgcc acacccattg cctcagcttt cgcggcgttg acgttcactt
     2101 tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt
     2161 gcgctgccag tgataacagc tcgtttcgtg cctgggttag tttttcgtgc cccaggttac
     2221 cgttgtccag cagttccata tcaaaacctg acgcggtacc cagttcagcc accgcgggta
     2281 agttgaacgg gaagacgacg gctttattga tactgcttaa cgcaatcatt gcccgctgaa
     2341 tgatcgcggt aaccgagttt tcctcaccga cacgttcaga ccacggcttg agactgataa
     2401 acgccaggcc gttgttttgc ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg
     2461 actgaacatt atctttctct tttgtaagat aataatccgt cacctgttgc agcactttcg
     2521 tggtgttaac catggtggca ccggaaggta actgcgcggt ggtcataaat accccctgat
     2581 cctcttctgg taagaaagag gtcggtgtac gcaggaacag caccgccatc ccggcgcaaa
     2641 tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg
     2701 tatagtgctg agttgatttt tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt
     2761 tgtgaccgcc ttccggcgcg gctttcagaa tggtggcgca cagggcaggg gtcaggctca
     2821 ttgccacaaa tactgaaagc agcatggagg agatcagcgt gatggagaac tggcggtaga
     2881 tctccccggt tgcgccgctc ataaaggcca tcggcataaa cactgcggaa agaacaacag
     2941 caataccgac cagcgcacgt tggatctgcc ccatcgattt atgcgtcgct tccttcggtg
     3001 gtagcttatc ttccgcaatg acacgctcga cgttctccac caccacgatg gcgtcatcca
     3061 ccagtaaccc tatcgccagc accatcccga acatagttaa cgtgttgatg gtgaaaccga
     3121 ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg tacggcaatc gtcgggatga
     3181 ttgtggcacg gaaattctgc aaaaacagat acatgaccag gaagacgagg atgatagcct
     3241 caaccagtgt tttaaaaact tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag
     3301 gataaaccgt cttcagactt gcggggaaat aggctgataa gcggttcagc tcctctttga
     3361 ctgcccgcga ggtatccagg gcgtttgctc cggtagccag tttgatggcg atcccggcag
     3421 caggtttgcc gttatagcgc gccacggtgg aataatcttc cgctccaagt tcgacgcggg
     3481 cgacatcacg cagcagcact tgcgaaccat cttgctgaac tttcaacagg attttgccaa
     3541 attcttccgg cgtttgcaga cgcgtctgca caatgatcga ggcgtttagt tgctggtctg
     3601 ccgcctgtgg catgccaccc agttgaccac cggaaatctg gttgttttgc accttaatct
     3661 gggaaataac atcggaaggt accagattgt atttattgag tttttgcggg tccagccaga
     3721 tacgcatggc gtactcggaa ccaaagagct gtacgctacc aacgcccgcg gtacggctta
     3781 gcgggtcttt gatattagac gctacatagt ccgcgatatc gtactggttg aggctgccgt
     3841 tatcagaaat aaacgccgct accatcagaa tattactgct cgacttatca acgctaatcc
     3901 cctgctgctg caccgcttca ggtaatgaag gcatagcgag ttgcagttta ttttgcacct
     3961 gaacctgtgc gatatcagga gatgtcccag tctcgaaggt cagagtgata gaggcattac
     4021 ccgccgcatc actggttgaa gacatgtaca tcaggccatc aagcccattc atattttgct
     4081 caatcacctg agtgaccgag tcttctaccg tttgcgcatc ggcacctgga taggtagcgc
     4141 tgacggtaat ggtcggtggc gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg
     4201 ccagaccacc tgcaagcatc ataataatgg caagtaccca ggcaaaaacc gggcgatcaa
     4261 taaaatagtt agccatgaaa gtccccttaa gcctgcaacg ttattgtttc gattcggtgc
     4321 tggcgttttc ctggctggag gaaattgctc gtgctttgat acccggacga atgcgttgca
     4381 aaccggaaac gatcacccga tcgccagcct gcaagccaga ggtgacaacc cactggtcgc
     4441 cgatggcttt gctggcttca atttcgcgta gctgcacaac atcgtcttta tccagaatga
     4501 gcgccgttgc tttaccctgg gcgttgtggg tgacgccttc ctgcggcacc agtaatacat
     4561 tctggcggct accttcatcc actaatgccg tgacgtacat gccaggcagc aagtcaccat
     4621 ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt ggtttcatcc accgtcgggt
     4681 cggagaattt cagcgtgccg gtctggctgt agcgtttacc attttccaga ttgagctgta
     4741 ctggcgtact gccctgaacc tgtttgattt gcccactggc gacctcttct ttcatgcgta
     4801 agaaatcttg caccgactgc gtgagatcga cataaatcgg gtccagacgt tgtacggtaa
     4861 ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc
     4921 tgacgcccgt aatcggcgag gtgacattcg cgtattgcag attgatcgtc gcctgttcaa
     4981 cagccgcttt ggcgacggtg acattggctt ctgcttcatt caactgggtg cgcgcggtat
     5041 cgtaatcctg acggctaacg tagttggtct tcagcaacga tgcctggcgg ttaaaggtga
     5101 tgcgggcatt gctggcggta gagagcgctt tcgccagcga gcctttggcg gagtttagct
     5161 cggcctgtaa aggtgcagga tcaatctgat acagcgaatc gccctggttc actttatcgc
     5221 cttcgataaa gttgcgttta atgataatac cgcccacctg gggacgtatc tcggcaactt
     5281 cataaggaac ggttctaccg ggcaattcgc tcaacacatt gaccgaaccg ggggagagtg
     5341 tgacgacacc gacctcaggc gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg
     5401 cggtgagcat cgcgccgcag aataacaacg gtattaacag ctttcttctt ctgttcattt
     5461 tagtccctga aaattcttga gaaataacgg ttctttaaat tgcgcgcgag gagagatatg
     5521 agggatttac agaccagcgg catagtgggg ttgtcagcca gtaaggtggg atacaggcac
     5581 agtgatcgac atggtgaggt caacgacaaa aaaaatgttg ccgttctgcc aacagttcct
     5641 gccagcattc gggcaacgaa gggttctact ggtggataca cataccaggg gaataaagtc
     5701 gttttagcct ctcctttagt aatcaccggg ggtaatgaaa tcagcatctg catccctcgt
     5761 catgccagcc atcaatttca gttgcttatg tcctgactaa aaataagatg tgatacccag
     5821 ggtgacgatg tcgctcatac gtcttagttt cagcttgtac ataatgttga atttctgaat
     5881 ctttaatgtt tgttgcgaaa tattgagcgt cgacgtgata ttgctttcat ttttattttt
     5941 cagaatcagt tgcataattt tgaactgatt atgtgaaaaa cataattgtg aattcttatg
     6001 gggcaagtgt ttaccataag taatctcaag aagatttttg atactttctt tgcggctaac
     6061 aaaataaata ggcgctttag cttttagttt actgatgtga taaggtgcca aaacaataat
     6121 tctctcggca tctaatttct ccagaaattt aatcgcttct tcatcaagga tatgattgtg
     6181 ataggtatca atgataacct tgaagggttt attgcctact tttttgatct ctgacaggga
     6241 attgattttt atcatttcgt gattatcttt caactgccaa aagccctgta aaagaaaaga
     6301 atctttcgtc atgagaaaaa tcataacttg ctccttagcc gttatcgttt cgaacatgtc
     6361 atccttttca ttatttacat ccttgtccga atcgttgttc aatatagtaa acgcctataa
     6421 gaactttgcc cttcgtgaac taatacgtct attaacacta atcaatactt cagatttatc
     6481 catcacgcac tccatatatt gatcctaagt aagattcctg gttgttatca atttgtaaat
     6541 atcaaacaaa aagttaacgt ttgcggatta ctggccaaga ataagttatc aataaccagt
     6601 ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat ccgatttaaa tatcgagtct
     6661 ccttgtttcg acttaagctg gcaattggat tgccagcttt cttttaacgc attgaaatta
     6721 cctgagtaaa taacgcattc atgtcttcag gtttttatat agcgtctgga gacacggata
     6781 tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac ccggattact tatttaaccg
     6841 cccatagcat caacgcttac ggaactcttt gttcactttc ccacagcact tttaaaacat
     6901 taaacctgac tacggaaaat atcagccatg ataatgtttg aaatggctaa tttgccatgg
     6961 agtgaaaaaa attagatgaa attcagtagg ttgaaatgat cactagcagg taattatttc
     7021 aatgatagtg cgcaattgat ctacaacact gcgtagcgga gagagtatta atcggatcat
     7081 agtcacatca agtgactatg atcctggtga caaccgggat aattattgct gcttaacgaa
     7141 caaactggcg aagctgaaca ggctggcggc gctaaatatc agttcaattc ccaccagtgt
     7201 ggaaaccagc gttacagaca ccatcggcgt tgcaccaagg aatatccagg caatgacgat
     7261 atccagcaca ccaataacga gctgtagcca gctgcctttc attgaacgct gacgatacca
     7321 actcatcagg cgaataaccc ctgcaacaca gaacaaaccg gcaataaatg ccgcaatggc
     7381 aaaaatgccc agctccggtg cgcggatgaa gaaatagccg atcaataaat aggcgactgc
     7441 gacgaggaaa ccggataata ccggccagaa attatgactg cggttgctga ataacccgac
     7501 aataagcgca atacccgagc agattaataa tgcacccact actgtgctta aaatatcgcc
     7561 agagacgaac gggaaactga tacacagcaa cccgacgata aacagcagca cggcaataaa
     7621 ctggattgct ctgcgatgct ttttcagcat ctccagatca aacttcaaaa ttgttgcctt
     7681 atctatatat aacatagaac caccctataa aattaagaag aaaatcccct gctatcaatc
     7741 tatgccaaaa acgcgtctaa gaatgcagtc gatttaataa aaatttccta attgcagtat
     7801 ctgatgcatc tgtaactcat tgtattgaaa taaaaatatc tgattttgat attttccatc
     7861 aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat tcgtgacggc
     7921 tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt attcttggtg
     7981 gtctgcttct tctgccagtt gtgagcaatg cagcggatgc gcaaaaagca gctgataaca
     8041 aaaaaccggt caactcctgg acctgtgaag atttcctggc tgtggacgaa tccttccagc
     8101 caactgcagt tggttttgct gaagcgctga acaacaaaga taaaccagaa gatgcggttt
     8161 tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt actcaggata
     8221 aacaagccaa ctttaaagat aaagttaaag gcgaatggga caaaattaag aaagatatgt
     8281 aattccggga atgcgttaca tcgtacttcc ttgcatattg aacaggccgg aatatcttct
     8341 ttaaaagcag ctattcctcc tgttcatata taatctctat attgaatggg ttacaaaatg
     8401 aatatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc tttgtcactg
     8461 gtgaacgcac aatctgcgct ggcagccaat gaatccgcta aagatatgac ctgccaggaa
     8521 tttattgatc tgaatccaaa agcaatgacc ccggttgcat ggtggatgct gcatgaagaa
     8581 acagtatata aaggtggcga taccgttact ttaaatgaaa ccgatctcac tcaaattcct
     8641 aaagtgatcg aatactgtaa gaaaaacccg cagaaaaatt tgtatacctt caaaaatcaa
     8701 gcatctaatg acttgccgaa ttaatgaggt acaagtaaaa aggagtagca agttgagcca
     8761 tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg tcttatactc
     8821 gcggcaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa aggagcaggg
     8881 ttacgcacac atgtattaat tggcatggga agcgccctgt ttatgattgt ttcgaaatat
     8941 ggttttgctg acgtgctgtc tttagatcac gtcggactcg accccagccg tattgctgct
     9001 caggtggtga cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg taaccaaaat
     9061 attgtaggtc tgacgacggc agcggatatc tgggtgaccg ccgccatagg tatggttatt
     9121 ggcagcggta tgtacgaact gggtatttat ggttcagtga tgaccttgtt ggtgctggaa
     9181 gtcttccatc aattaacctt ccgcctgatg aacaaaaatt accatttaca gctgacatta
     9241 gtgaacggta atacggtatc aatgctcgac tggtttaagc agcaaaaaat aaaaacggat
     9301 ttagtttcgt tgcaggaaaa tgaagatcat gaagtggtcg ccattgatat ccagcttcat
     9361 gccacaacgt cgatagaaga cctgttgcga ttattaaaag gtatggcagg ggtgaaaggc
     9421 gtttcaatta gctaatctgt gtggcagcgt agccagactc accgtaagcc tgaaattcac
     9481 accagataat caatatgctg atggcgtacc agctcattga tccgcttaag attgaggatc
     9541 atcatgatat tgtgtttatg gcagtagaac gtttttagcg atattttcag tttctccaga
     9601 atatcttcag ttgtcatttg attcgcaatg cagctcagaa cctgatgttg cttgcgcgaa
     9661 agtgacacat tcgcttcagg cacagagcag taactgccat tgatgagtgc gacaaaatca
     9721 atgatagtga tagtccgcgg aacaaaggtc accggtactt tccccagaca gcgtttaata
     9781 ttaaaggggg agaaaacaat gacatggaca ggttttaatc gttcaatttg agtgagaaaa
     9841 ttagaatgaa atacattatt cattaatgta tcaataatga cgaaggcatt ctgatgcaac
     9901 attacgtcga tctcttcttc gttctgtata tgaacgacat tacctttact cagaatgttt
     9961 ttcatcgcgg tgaagaacat cgtatccctg gtaattataa gaaacataac cgactcctgg
    10021 tgcggacatt aactttataa tcagtttgcc aaaggtaatt aacaggacag ggcttataat
    10081 aataatagtt agcttactgt atattaattt tatgtaatac atgatgcaga tgctgagaaa
    10141 gcatctgcat ctttcggtgg tgttatttga ccagctcagg tgttacctga ctcaccgcat
    10201 tggtgtagta aggcgcaccc cagcccggtt ccggccagaa tgcaccatag ccgtaatccc
    10261 acaggttata ggtggtatta accacttctc tcaaatgcca cacctggatg ccctgcatat
    10321 tcacttccag gaagttatac gggactttat tgataaagcc tggttgttca ccctgaatgg
    10381 taccgaggat ggtaacaaag tgattacgat agttcactgg atcaaggaag ccgctttgtc
    10441 tggcgagcag tcggccctga tagttggctt caatatcagg cttcgcatag ctatccaacg
    10501 gtaatacagc gatttctaac aacgtatccg ttttgccatt gataacgttg ataaccttcc
    10561 caccaaagcg cgcttgttga ccaacatata accccggctg gttatgaaca gcaacaaaac
    10621 ttttttgaat atcaggttgg ttattgcctt tgatattttg cggaattgaa ctacatgcgg
    10681 caagcaaaaa tgaaaggctg aggatgagtg caccttttgt catgttcatg ttactatcct
    10741 tatcaacaaa tattaaagcc ataatagata tttctttata gttttcatct gattctgagg
    10801 tgatatgcct gatagtaacg taaacctgtc tgatgtttat atcccttttg tttagttcaa
    10861 ttccattgat tttggggc
//
LOCUS       DAZGTR010000139        10841 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGTR010000139 DAZGTR010000000
VERSION     DAZGTR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.141"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            61..711
                     /gene="csgD"
                     /locus_tag="ACHLCY_004085"
     CDS             61..711
                     /gene="csgD"
                     /locus_tag="ACHLCY_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP0371237.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            716..1105
                     /gene="csgE"
                     /locus_tag="ACHLCY_004086"
     CDS             716..1105
                     /gene="csgE"
                     /locus_tag="ACHLCY_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP0371238.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1130..1546
                     /gene="csgF"
                     /locus_tag="ACHLCY_004087"
     CDS             1130..1546
                     /gene="csgF"
                     /locus_tag="ACHLCY_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP0371239.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1573..2406
                     /gene="csgG"
                     /locus_tag="ACHLCY_004088"
     CDS             1573..2406
                     /gene="csgG"
                     /locus_tag="ACHLCY_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP0371240.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2470..2961)
                     /gene="ycdZ"
                     /locus_tag="ACHLCY_004089"
     CDS             complement(2470..2961)
                     /gene="ycdZ"
                     /locus_tag="ACHLCY_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0371241.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3063..3617)
                     /gene="ycdY"
                     /locus_tag="ACHLCY_004090"
     CDS             complement(3063..3617)
                     /gene="ycdY"
                     /locus_tag="ACHLCY_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP0371242.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3641..4378)
                     /gene="ycdX"
                     /locus_tag="ACHLCY_004091"
     CDS             complement(3641..4378)
                     /gene="ycdX"
                     /locus_tag="ACHLCY_004091"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP0371243.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4433..5371)
                     /gene="ghrA"
                     /locus_tag="ACHLCY_004092"
     CDS             complement(4433..5371)
                     /gene="ghrA"
                     /locus_tag="ACHLCY_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP0371244.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5605..5692
                     /locus_tag="ACHLCY_004093"
     tRNA            5605..5692
                     /locus_tag="ACHLCY_004093"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5639..5641,aa:Ser,seq:gga)
     gene            complement(5842..6684)
                     /locus_tag="ACHLCY_004094"
     CDS             complement(5842..6684)
                     /locus_tag="ACHLCY_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0371245.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6769..6966)
                     /locus_tag="ACHLCY_004095"
     CDS             complement(6769..6966)
                     /locus_tag="ACHLCY_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0371246.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(6978..7469)
                     /locus_tag="ACHLCY_004096"
     CDS             complement(6978..7469)
                     /locus_tag="ACHLCY_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0371247.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7466..7840)
                     /locus_tag="ACHLCY_004097"
     CDS             complement(7466..7840)
                     /locus_tag="ACHLCY_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0371248.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7887..8261)
                     /locus_tag="ACHLCY_004098"
     CDS             complement(7887..8261)
                     /locus_tag="ACHLCY_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0371249.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(8424..8645)
                     /locus_tag="ACHLCY_004099"
     CDS             complement(8424..8645)
                     /locus_tag="ACHLCY_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0371250.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(8708..9184)
                     /locus_tag="ACHLCY_004100"
     CDS             complement(8708..9184)
                     /locus_tag="ACHLCY_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0371251.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRTAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(9200..9685)
                     /locus_tag="ACHLCY_004101"
     CDS             complement(9200..9685)
                     /locus_tag="ACHLCY_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0371252.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(9776..10594)
                     /locus_tag="ACHLCY_004102"
     CDS             complement(9776..10594)
                     /locus_tag="ACHLCY_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0371253.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
BASE COUNT         2728 a         2709 c         2715 g         2689 t
ORIGIN      
        1 tgcttctatt ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg gggtttcatc
       61 atgtttaatg aagtccatag tattcatggt catacattat tgttgatcac taaaccgtct
      121 ttgcaagcga cagctctctt gcagcacctt aaacaatcgc tggcaattac aggaaaatta
      181 cataatattc aacgttctct ggacgatata tcttcaggct ctattattct tctggatatg
      241 atggaagcag ataaaaagct tatccattat tggcaggata ctttgagcag gaaaaacaac
      301 aatatcaaaa tattgttgct aaatacgcct gaagattacc cgtaccgcga cattgaaaac
      361 tggcctcata tcaacggcgt tttttatgcc atggaggatc aagaacgtgt tgtcaatggg
      421 ttgcaaggcg tcctgcgcgg cgaatgctac tttacgcaaa agcttgccag ttacctgatt
      481 acacattctg gtaactatcg ttataacagc acggaatcag ccctccttac tcatcgggaa
      541 aaagagatcc tgaataagct gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg
      601 ttcatcagcg aaaatacggt taaaacgcat ctttataatc ttttcaagaa gatagccgta
      661 aaaaaccgga cacaagcggt ttcctgggca aacgataacc tcaggcgata aagccatgaa
      721 acgttattta cgctggattg tggcggcaga atttctgttc gccgcaggga atcttcacgc
      781 cgttgaggta gaagtcccgg gattgttaac tgaccatact gtttcatcta ttggccatga
      841 tttttaccga gcctttagtg ataaatggga aagtgactat acgggtaact taacgattaa
      901 tgaaaggccc agtgcacgat ggggaagctg gatcactata acggtcaatc aggacgttat
      961 tttccagact tttttatttc cgttgaaaag agacttcgag aaaactgtcg tctttgcact
     1021 gattcaaact gaagaagcac taaatcgtcg ccagataaat caggcgttat taagtacggg
     1081 cgatttggcg catgatgaat tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa
     1141 acatgcagta gttctactca tgcttatttc gccattaagt tgggctggaa ccatgacttt
     1201 ccagttccgt aatccaaact ttggtggtaa cccaaataat ggcgcttttt tattaaatag
     1261 cgctcaggcc caaaactctt ataaagatcc gagctataac gatgactttg gtattgaaac
     1321 accctcagcg ttagataact ttactcaggc catccagtca caaattttag gtgggctact
     1381 gtcgaatatt aataccggta aaccgggccg catggtgacc aacgattata ttgttgatat
     1441 tgctaaccgc gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc
     1501 gaccatccag gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc cagcttcata
     1561 aggaaaataa tcatgcagcg cttatttctt ttggttgccg tcatgttact gagcggatgc
     1621 ttaaccgccc cgcctaaaga agccgccaga ccgacattaa tgcctcgagc tcagagctac
     1681 aaagatttga cccatctgcc agcgccgacg ggtaagatct ttgtttcggt atacaacatt
     1741 caggacgaaa ccgggcaatt taaaccctac ccggcaagta acttctccac tgctgttccg
     1801 caaagcgcca cggcaatgct ggtcacggca ttgaaagatt ctcgctggtt tataccgctg
     1861 gagcgccagg gcttacaaaa cctgcttaac gagcgcaaga ttattcgtgc ggcacaagaa
     1921 aacggcacgg ttgccattaa taaccgaatc ccgctgcaat ctttaacggc ggcaaatatt
     1981 atggttgaag gttcgattat cggttatgaa agcaacgtta aatcgggcgg tgttggggca
     2041 agatattttg ggatcggtgc agacacgcaa taccagctcg atcagattgc cgtgaacctg
     2101 cgcgtcgtca atgtgagtac cggtgagatc ctctcctcgg tgaacaccag taagacgata
     2161 ctttcctatg aagttcaggc cggggttttc cgctttattg actaccagcg cttgctggaa
     2221 ggggaagtgg gttacacctc aaacgaaccc gttatgctgt gtctgatgtc ggctatcgaa
     2281 acaggggtca ttttcctgat taatgatggt atcgaccgtg gtctgtggga tttgcagaac
     2341 aaagcagaac ggcagaacga cattctggtg aaataccgcc atatgtcggt tccaccggaa
     2401 tcctgacaga taaaaaagcc ggaattatca tccggcattt tactgtttaa gcgaaagcgt
     2461 gagtgcccct cacgctttat ttttgatttc ctgctcacgg tgagccgtct ttgcactacg
     2521 cgccgccagc cacaggccgc tgtttttcat tgcgtaacca aataccagcc caagcgccag
     2581 cgaaggtaac accagtttcc agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa
     2641 cgtccccgga acaaatgaaa gcagcagctg tttggcctga atacacatca gaaacgcgac
     2701 aataccggtt atgacataac cgagaatttc cagatgtggt gccagtgcac taccgtaaat
     2761 aatgaccatc gcccacacca cgccactaag cagggttgca gcggagatcg ccagcccttt
     2821 cagcccacct tgcgggcagg caaagtaggc cgtacagccc aggaagcccg cccagcttag
     2881 taagcccagg gaaacagcca cccatcccca gataccggag agaatgcctg ttgtgattgc
     2941 aatagagaga agtatgttca tgccgcgcat gatagcagaa aacgcggcca tgaatgagat
     3001 cgagcacaca ttttatgcaa caacaaatta tctgttgcag ttatgagtga cgatgatcac
     3061 atttactctt cagaatcttc ttccagctcg tcccacattg cactaatggc atcacgggtt
     3121 agcggtgcca tggtgcgcca gaaaggcgtg gttgcatggg cctccacttt gccgaggaac
     3181 gcaccacacc agggtaacag atactcactg aacagtgttt ccagtgcttc gctctcatct
     3241 tccgttgact gatcttccag ccaggaagcc gcaagcagca atgtgccgat gtgatcggct
     3301 ggcgtatccg ccagtggcat ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt
     3361 gcgtcctcaa cccatgcgct acgatatggc ggcacggcac attcatcgcc gataaacaac
     3421 gcattgtaat cggcagagac ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac
     3481 tcatcctgct ccagtggcca gttcgcagcc agtttcccct cacgaatcag ggtaaacagc
     3541 ggcaccagta aaggatcttg cggttggcgg taatagagcg aacccagcac acgacagagg
     3601 atagaaaact cgttcattaa tatatttcca ttactagtaa ttaaaggtct gcaaattccg
     3661 caatcggtgc cataccgcga gattcaagga agttcagtaa gcggcgcggc gaaacattca
     3721 aaatgcgctc tggcggaaaa tctaccgcgt cgaggatttt aagacactct tcaaattccc
     3781 ccatggtaaa cgcggtgtga gaatccgagc ctaatgccac ccaacctcca gcatcacgta
     3841 ccgctgcggc taccgcacga cagttgtctt cactgccctt acgtgagtgt aaaaatgaag
     3901 aattattgat ttccagcgcc acctgatgtt tcgcggctgc ttcagcaacg gctttcacat
     3961 ctatttcata tttgggattt ccgggatggc ttattatatg cacattgccg ctggcgatag
     4021 tggcgatcat cgcttgtgta ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa
     4081 aaccggcaat aatcagatcc agcgagtcaa acattttacc gctgcaatca atttcaccat
     4141 caacattttt aatgttagct tcgatgccgc gcaggatccc taccccatca accactcgcg
     4201 gccagatacg catgttaatg aagtgccagt gatgcggagc atcttccata tccgggccat
     4261 gatcggtgat cgcaaaaagt ttaatgccct tttgtttggc ctgggcaatg taatcactca
     4321 atgtgctata tgcatgtgtg ctggcaacgg tatgcatatg aaggtcgacg ggatacataa
     4381 ctctctcctg tattcaattc ccgccaagga tagcaggaat cctgatgctt tattagtagc
     4441 cgcgtgcgcg gtcgacttgc ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc
     4501 gagaaatgta ctccacagct tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg
     4561 tcacgcgtgg atgttgccag agcggacttt caggcggtaa gggttcacga ttaaaaacat
     4621 ccaacattgc gcctttaact ttgccgctat ccagcgccgc gagcaggtca tcttccacaa
     4681 catgaacacc acgcgccagg ttgaggagat acgcgccatc cggtaatttt tcgagtaatt
     4741 gttgattaat aatgccgacg gtttcagggg tattcggtaa caaattaatc aatacccgac
     4801 attggctcag aaatgcagac agttcttccc gtccggcaaa gctttgcacg ccaggccacg
     4861 atttacgggt tcgactccag caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa
     4921 ctttactgcc cagtacgcct gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt
     4981 caggcagcgg ttgccaatgc gaactatttt gctggatgcg ataatcgtca aaacgtcgaa
     5041 accaatgcag cacctgactg acagcatatt cctgcatttg ctcgcccata ccggtatctt
     5101 ccaggcgaaa aagtggaaca gaagggttca gcatttcagg gtgtgcctgt agcttgctca
     5161 aaatagaatc aacaccggcc ccgagtgcga acaccgcttt aagatcgcgc cctgccagca
     5221 tttcaacagg aggatgccag actaaagcat aatcagcaga gtcattatct ccgcttttcc
     5281 acgctctgac tcttgcctga ggaatagctt tgcgcagtgc ctcaatccac cattgggtat
     5341 cgaacgttgg gtgataaaag atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat
     5401 ttattcgcac ttatcgttat gatctacaaa ggccaccagc ataacaaatc cgtggtcggt
     5461 ggcaaaagaa gcagatttcg cttattaaaa ccacacattg attgaagttt gaataaacgc
     5521 gcgatttttt caaaaagttt gttgacctca ggtcatgatt tccctaaatt agcgcccgtt
     5581 ccagcaagac aggaacgaca atttggtgag gtgtccgagt ggctgaagga gcacgcctgg
     5641 aaagtgtgta tacggcaacg tatcgggggt tcgaatcccc ccctcaccgc cagattataa
     5701 gcgcatgtta ttgtttaaca tgcgcttttt tattttccct tccagacagt atgccatcgg
     5761 gtatgccatc tgaaaccggt ttatatgccc cgcgatcata tcttatcgga caatttccga
     5821 tctttgagca tactggcaaa gctatactgc cagcatatca ggatagtgtt ttgcgataat
     5881 atcattcagt ctgtcaacca gttctggttt tctgaacgtg atatgcgcgg agcccttctt
     5941 aaagtatctg atgctgaaca tctcatcttc atagctctct ttaccctgaa cagactgaat
     6001 gtgatcatcc agacgaaggg tgatgtttcc ccggttgtcg gggattggtt taccgctgaa
     6061 caggtgcaac attctttcca gatcggcgag tcggtcagcc tgttgtccgg tgataagatg
     6121 aaatccccac cggtcccatc tcaccagatt gttgacgata attttactgc caaatttaca
     6181 ggggcaattg gttttgtaat tccagttcag ccctctgaag acgttgatca cccctcgctc
     6241 aaacacctca tccttattct ggtgcagttg ttcaaatgtg ctcagaatgt tcgcttcgct
     6301 gatttccgga aaattatcgt actccagtga ccggtaccat gtatcacggg cctgcgtatc
     6361 cattaatgac agcatcccgg aacgttgcat caggtcacgc cagatttcgc gatcaagatt
     6421 ccgggtaatg gctttcattg ctgtttcggg tttctccatc agccagcagc cgcagcgaaa
     6481 atcctgtttc atggcccagt ccggggcagt tttacctccg atgctttccg tcagcaccga
     6541 gatttcagcc agttgtcgga taagtgtgtc gatttgcaac agtgtactct ttcgtacagc
     6601 cagaatgtgg cgaatgctgg ttgagcagat aacgtccgta tgcccgttga ccacttccgg
     6661 acgggagatt gtgatatctg acataatcga tattcctttt tgcagataaa tgaaaacgcc
     6721 agccctgtgc aggctggcgt cagaggaatg aagtctggtg agtgagcttc accggttctg
     6781 gtgcagaagc tgcagatgac gcagggtacg gacatcctcc cgggcctgtt cgatacaggg
     6841 caacagtgct gctgaatccg ttttatcctc atcgttgtca aagataattc ccgatccgca
     6901 gtcgatattg tcctgcagcc acgcaatcag aatatccagc gctgtttccg tggttaatga
     6961 tttcatgttg ttcctggtta ccagtcgaaa gtgggtaaac ccggcaggca ttcagcgctg
     7021 gcatccagat gaatgagact gacgccataa cgccggatga gtgtgacgac cagacgacgg
     7081 aacgtttttg acagccccag gcgtttaagc cgcagaaccg ggtatgacca cgcatcggta
     7141 cgtaacagat atccgctacc tgtaaaatga atccattctg attcaccgaa gtcactggtc
     7201 tggtgtgaca gcgagtacaa ccaggcgttg tccgtttccg tgatatgtgc ggtactgcag
     7261 cgtatgccgg taagggtagc aaatggtggc gtgcattctg ctgaggtctg gtcagattca
     7321 tcaacaacac gaagggagta gccgttatcg cagaccatgt taatcagttc agcgagctca
     7381 ataccatcga cgtcaacgac aatgcgcccc atgttcagtg cctgtacgtt aacgctgtcg
     7441 gcttccagtg tcagggccag tttcatcatt tcgcctccgg atacttaccc agggtaatgt
     7501 tatttaccgt tctgtaattg tcgcgggtca tcaggccggt cgcccggcga gcccggagga
     7561 tatcaatact gtttattaac tgagagcggg gacaggtgct gaatcccggc tggtcggtac
     7621 gcaccagcgc gtatttttcc acgagaaaat taactgcatc acacagtgaa atgcctgcct
     7681 caatatgctg ctcaatcaca cgttcatcgg caaacggtgt gtcattcagt gtgaggccat
     7741 aatgctggtc cagcagtcgg atgagcagtg tctgccagat ggtgacggga gacgggcagc
     7801 gagatacctc ccgtacatgt gtgtcggata atgttttcat cttaagttct ctgtaactgg
     7861 tagtgctaaa ggtgagagct tcggggttat gaggtggttg cgggtgctgg tgtcggataa
     7921 acagccagat aaacgtaacc acaggagccg agggtgtcgg cttcgcaggt cagccctttt
     7981 gcattcagcg tgacggtatg ctgatatcgg ggattcagtt caccgctggt gagcatgagt
     8041 tccagttgtt tcatcagcag cggaaaggcc tggtccagat ggtatgcatc tgcgtcgctg
     8101 aagcggcctc tgataccggc gcggtcggca aggtaatgca ggcggttacc ctcttgcacc
     8161 agacgagcgc caaaacaggg cctcactgtg cagggaagcc cccaccaggg gcggtcgtga
     8221 ttgtcatcgg gatgagttat cccggagagt ttgtctgaca cgataaaatc ctctctgaaa
     8281 agtggttaag aatggtttaa cgatgacagg tcagccggcg cgctgaccgg tttcggtgaa
     8341 gatgtatgca ttgatgagaa tggcctcatc cacagcggcg tctgaggtca tctggtcgta
     8401 ctcgtttccc gggtgacgga acatcagttc agggtgacac tcatcagccg gacatacggg
     8461 ccaaaactgt ccttacggcg ttctgcaaac acggcaagaa cgccgggaat atcctgcact
     8521 tcacgaccag tatacgcttc agcactgccg tgccagcgat acttaccggt acagaacgga
     8581 aaaagacggg atgcaggatg ctgttggtga atacgcatgg cttcaccacg ggtgatgatt
     8641 ctcatgatgt gatacctctg aagacagaag ataaaagtga aaacaggtgt gatgtggttg
     8701 tgacgggtta aagcagacca tgttcggcaa aggagaaaac ctggttgcca ccgactatca
     8761 gatggtccgg cacccggata tccaccaggc ccagtgcctg taccagacgt tccgtgataa
     8821 ggcggtctgc cttgctgggt gtgacttcac cggacgggtg attgtgtgcc agcaccacgg
     8881 ctgcggcatt gtggtacagg gcgcgtttaa tcacttcccg gggatggact tccgtgcggt
     8941 tgatggtgcc ggtgaagagg gtttcaccgg caatcagctg attctggttg ttcagataca
     9001 gcacccggaa ttcttcacgc tccagtcccg ccatgttcag aatcagccat tcccgtgccg
     9061 tacgggtgga ggtgaaggcc acgccgggtt catgaagatg gcggtccagg gtttgcaggg
     9121 cccgcagaat gagactgcgc tcgccgggcg tcatctctcc gggcagaaag gaaagctgct
     9181 gcattgtccc tgcctccatt cagtcaatga tgcgcataat ggcgctgcat tccggatgct
     9241 gcagggcgta atcccgcagc cggtaatagt ggaccgtcat ggcataacac tccgtacgac
     9301 aggcatgatg actgtacgtc atcagacagg cagcaatacc ggcggcttcc gggctcattt
     9361 cagcgcggtt gccgttcatg gcattgaaca gtacccatgt ttcgtcatca tcgtcatccg
     9421 gttcgggtgc cataaatgcc ccgccgttgt tcagggtgta cagattccag ataccaccgc
     9481 agtagtcttc gcaaagacgg tccatccagc cgaagacacg gggctccagg gtcacccact
     9541 gtggaatgag gccaaaatgc tgcggccaga agctgatgcg ctgttcatca gggacgggag
     9601 tggcaaccag ctgaggtggg ttattccctg atgcagtggt tacggaaaca gaaggtgtgg
     9661 tggaattatg cgaaacggtt gtcatgaggt tattccttat aaaaagtaaa tgaatggaag
     9721 aagccccggg gcaggaacag atgtgagtca ggactgcact ttcagtgaaa cggcatcagc
     9781 gcatgctctc cagcagcgtt tctgccatca cccacaatgc gcgattgagc ttaatgtcgg
     9841 tatcaatgct gtgaatggcc cgggtatgga tacgttttcc ttttgcactg cgaccggaaa
     9901 tcccgccttt cagcatattc tcctggatgg tctgataagc actccacagg tccttaccgt
     9961 aatcctcccg gcgtcgtggt gtcagaatgt cggcggtggt gacgggctga tgttcgtcac
    10021 cataacggta agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc ggcaggacca
    10081 gcgactgcat ggcatcacgc ttctcctcta tccggtcaaa aacgcccacc acctcgtaag
    10141 ccccttcaat gactctgtcc accacatttc cccggtgtgg aacacgcaat tctcccagag
    10201 actgaccgca gaccagtcca ttggtacaaa tggcacgaaa atatccgggt aacatctggt
    10261 agctggatga accgtcatgg gagttgagca gaataatttc agggacatgc tgaccggtta
    10321 tctgtccggc ccgccgcaga cgcagcatgt gttttgtgta ttcccggcgg ctctggtcgc
    10381 gcacgcgggt ctggcaggca aagaacggct gaaagccttc ctgctgcaga ttttccagga
    10441 cggtgatagt gggaatgtac gcatagtgtt cactgcgaga ggtatgtcgg ttttccccaa
    10501 aaatactcgg tacatggcgc atcagttctt cgtgtgtcag cggacgatca cgacgtatct
    10561 ggttcgcata accaaaacga ctggctaatc tcatgatttg ctccttatga atgattaagc
    10621 gatataaatg aaaaagccgc gtctcccgga gaagacgcgg cctgatgggt gaaatgaatg
    10681 aggtttattg tctgagaagc ccttcactgg cgaaccacgt attaagctgt attccggcat
    10741 cgccagtgca actgaccttc agcattgcgg ataacccgcc ggaaatatgt tcactggttg
    10801 tttttgtatc ttcccccaaa attaaataaa ttcatcttaa c
//
LOCUS       DAZGTR010000140        10836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGTR010000140 DAZGTR010000000
VERSION     DAZGTR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.142"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHLCY_004103"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHLCY_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP0371254.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHLCY_004104"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHLCY_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP0371255.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHLCY_004105"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHLCY_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP0371256.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAISVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHLCY_004106"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHLCY_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP0371257.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHLCY_004107"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHLCY_004107"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP0371258.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHLCY_004108"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHLCY_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP0371259.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHLCY_004109"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHLCY_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP0371260.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHLCY_004110"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHLCY_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP0371261.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8629..>10836)
                     /gene="crfC"
                     /locus_tag="ACHLCY_004111"
     CDS             complement(8629..>10836)
                     /gene="crfC"
                     /locus_tag="ACHLCY_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP0371262.1"
                     /translation="ELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLALPWSQPNI
                     AERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPMTALPTLI
                     RHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRALMQRIENG
                     VAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVEFVHLAGL
                     ESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISDEEVREAI
                     LAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVSSMWGYLA
                     NRARHELANNGKLPPPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQLWEDSLF
                     AQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQLRQNIHQ
                     VEESLQLLQLNQAQVSGEIKHEIELALASANHFLRQQQDAVNAQLAALFQDDSEPLSE
                     IRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELSESGFRPG
                     FHFPVFHGAVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPDWGRGNEK
                     SPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFASCLTELQT
                     RLRESLALRQQNESVVRLMQQQLQRTVMTHGWIYTDAQLLRDDIQTLFTAERY"
BASE COUNT         2700 a         2836 c         2686 g         2614 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt atttgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagtt gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc tactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcgtacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggccgttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggcacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggtca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggctgtt actaccattg ccttcctcta ctgcatttgg
     2161 gccgtggtgg ggtccggggc gaaagaggtt atgtggtcat ttgtcaccct gatggtaatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctgcataaaa acccgtatcc cttagatgta
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca atttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggattga ttctggcggc gcaaactgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccagtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgctgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtgcgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcactgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttaaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagcg ccgttagtgg cacgacttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tgtttggtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcgac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccactacgcc agcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggtgagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc
     7261 agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aattggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgagcatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaatgccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgatc acctgcctgc accagtaggc
     8221 ctcgttgcgt taacgccttt tccttccagc ggcaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gtgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccaatgtc agacgcaggc taagcggcgc atttaacatg ccgccgttcg
     8461 tccatgtctg aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtcaagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtccgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccagacttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aaagctcgtt gcaacacact
     8941 gtaatcaacc gtctctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcttc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg aagtggggaa ctgcgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt ccagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacga atctcgctta acggctccga atcatcctga aacaacgcgg ctaactgtgc
     9361 attcaccgca tcttgctgtt gacgcagaaa gtggttggcg gaggccaagg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cggcagatcg
     9601 cagagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc ggtggtaact taccgttgtt ggctaactca tggcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactt tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattttt gaattaaacg gtcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttct ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attacgatta ggcaaaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaatcg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg
    10741 cgcatcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactc
//
LOCUS       DAZGTR010000141        10811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGTR010000141 DAZGTR010000000
VERSION     DAZGTR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.143"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(112..1158)
                     /gene="aroH"
                     /locus_tag="ACHLCY_004112"
     CDS             complement(112..1158)
                     /gene="aroH"
                     /locus_tag="ACHLCY_004112"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP0371263.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1315..2148)
                     /gene="ppsR"
                     /locus_tag="ACHLCY_004113"
     CDS             complement(1315..2148)
                     /gene="ppsR"
                     /locus_tag="ACHLCY_004113"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP0371264.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2481..4859
                     /gene="ppsA"
                     /locus_tag="ACHLCY_004114"
     CDS             2481..4859
                     /gene="ppsA"
                     /locus_tag="ACHLCY_004114"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP0371265.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4916..6562)
                     /gene="fadK"
                     /locus_tag="ACHLCY_004115"
     CDS             complement(4916..6562)
                     /gene="fadK"
                     /locus_tag="ACHLCY_004115"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP0371266.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGIEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6619..6912)
                     /locus_tag="ACHLCY_004116"
     CDS             complement(6619..6912)
                     /locus_tag="ACHLCY_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0371267.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6909..8198)
                     /locus_tag="ACHLCY_004117"
     CDS             complement(6909..8198)
                     /locus_tag="ACHLCY_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0371268.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGSKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8254..9192)
                     /locus_tag="ACHLCY_004118"
     CDS             complement(8254..9192)
                     /locus_tag="ACHLCY_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP0371269.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9212..9976)
                     /locus_tag="ACHLCY_004119"
     CDS             complement(9212..9976)
                     /locus_tag="ACHLCY_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP0371270.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDIEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            10292..>10811
                     /locus_tag="ACHLCY_004120"
     CDS             10292..>10811
                     /locus_tag="ACHLCY_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFP0371271.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQ"
BASE COUNT         2612 a         2753 c         2788 g         2658 t
ORIGIN      
        1 ataacaatga ttcttattat caatattatg gctgaccaga gcaagcagta atgtgctgag
       61 aagacaacaa gttgcgcaga aatgcccatc ccatcaggaa tgggcacgca ttcagaagcg
      121 ggtatctacc gcagaggcaa gtttttcgac caggcgttcg gtatcttccc agcccagaca
      181 cgggtcggta atggattgac cgtaagtgag cggctgaccg ccgacgattt tttgcgttcc
      241 ttcgcgcagg aaactttccg ccataattcc agcaatcgcc gtagagccat tgcggatttg
      301 ctgacaaata tcctcacaaa cttctaactg gcgacggtgc tgcttctggc agttaccgtg
      361 gctgaaatcc accaccagat gttcaggtaa atcaaactcg tgcagcgtat cgcaggctgc
      421 ggcgatatca tcggcatgat aattcggttt tttgccgcca cgcataataa tgtggccata
      481 cgggttgccg ctggtctgat agatggtcat ctgaccattt ttgtctggcg agagaaacat
      541 atggctggcg cgggctgcgc ggatagcatc cacggcaatc cgcgtgttgc catcggtacc
      601 atttttaaaa cctaccggac aggagagtgc cgaagccatt tcacggtgga tctgactttc
      661 ggtagtacgt gcgccaatcg cgccccaact gattaaatca gcaataaact gaccggtcac
      721 catatcgagg aactcagtcg cggtagggac acccagctca tttacctgta aaagtaattt
      781 gcgcgccagc tccagaccgt gatttacccg atagctgccg tttaaatccg gatcggagat
      841 tagccctttc cagccgacaa cagttcgtgg tttttcaaaa taggtgcgca ttacgatttc
      901 cagccgtgac tggtactggt tgcgcagcga ctgcagacgg gtggcgtact ccattgcagc
      961 ggtgagatcg tggatcgagc aggggccaat aatgaccaac agtcgcttat cttcaccatt
     1021 cagtattttt tcaattctgc ggcgggagtc ggtgacatgg gtggcgacgc caggcgttac
     1081 gggataccgt agcgcgagtt cggcgggcgt taccaggctc tcaatacgcg cagtacggag
     1141 ttcgtcagtt ctgttcatta caagtctcag aaatttgttg cttctacggc aggactaccg
     1201 ggaagtgatg cgcatcacga taaaccaatc gcagctgatt tcaagtgcga ggtgtgtcac
     1261 acctcgccgg gtggttagca tgataacaaa aaaataagct aatgcactag ttctctagta
     1321 cattcggcgg ctaagcccca tgatatcgag gatcttggtg gcaatctctt ctaccgaata
     1381 attggtactg ttaatccacg ggatctgatt tttacggtac aacgcttcta cttccgcgac
     1441 ttccatcctg cactgacgaa gcgaggcata gcggctgttc tcccgacgtt cctcgcgaat
     1501 cgccgccaga cgttccgggt cgatagtcag gccgaacaat ttatgctgaa gcggtttgag
     1561 cgacgcgggt agcaccagat tgtccatatc gtcggcaata aaggggtagt ttgccgcgcg
     1621 gataccaaac tgcattgcca gatacagact ggtgggggtt ttaccacagc gagaaacacc
     1681 gagcaggatc acctgagcct ggtccagatt gcgcaacgaa atgccgtcat cgtgggcgag
     1741 ggtgtaatca atcgccgcaa tgcgcgcatc atatttattg agattattag ggttaaggcc
     1801 atgggtacga tgagcaatcg gcgttggatc cagtttcatc tcttgttgta gcggggcaac
     1861 cagcgcctga acaatatcct ggcaaaagcc ttcactttgc aagatgatgg cgcgaatctc
     1921 cggcaacacg atggagtaga agaccagcgg gcgcacacct gtctggtgat aaattgcgtc
     1981 aatctgatcc ttcactgccc gtgcacggct ctcattttcg acaaacggca gcgtaatgct
     2041 gctgatagtg acgggaaatt gtgacattac tgcgtgtcct aatacctccg cagttattgc
     2101 cgtaccatca gaaatataaa aaacgtggcg atcaacagca ttatccattt tgttcttccc
     2161 gtgatgcaga cataattcta tgaaagcata aattaaaaac gcacagaagc gtaggacgta
     2221 atgtctggtt tataaaatga accttcaatt ttatttttta tgaaaacagc atttcatttt
     2281 tatggtttcg tttataccga tggtttatgt ggaaattgtc gaatagagca ggtttgcgca
     2341 acgctgggat cagtcttaaa aagtaaaaaa atatatttgc ttgaacgatt caccgttttt
     2401 ttcatccggt taaatatgca aagataaatg cgcagaaatg tgtttctcaa accgttcatt
     2461 tatcacaaaa ggattgttcg atgtccaaca atggctcgtc accgctggtg ctttggtata
     2521 accaactcgg catgaatgat gtagacaggg ttgggggcaa aaatgcctcc ctgggtgaaa
     2581 tgattactaa cctttccgga atgggtgttt ccgttccgaa tggtttcgcc acaaccgccg
     2641 acgcgtttaa ccagtttctg gaccaaagcg gcgtaaacca gcgcatttat gaactgctgg
     2701 ataaaacgga tattgacgat gttacccagc ttgcgaaagc gggcgcgcaa atccgccagt
     2761 ggattatcga cactcccttc cagcctgagc tggaaaatgc catccgcgaa gcttatgcac
     2821 agctttccgc cgatgacgaa aacgcctctt ttgcggtgcg ctcctccgct actgcagaag
     2881 atatgccgga cgcttccttt gccggtcagc aggaaacctt cctcaacgtt cagggttttg
     2941 acgccgttct cgtggcagtg aaacatgtat ttgcttctct gtttaacgat cgcgccatct
     3001 cttatcgtgt gcaccagggt tacgatcacc gtggcgtggc gctctccgcc ggtgttcaac
     3061 ggatggtgcg ctctgacctc gcatcttctg gcgtgatgtt ctccattgat accgaatccg
     3121 gctttgacca ggtggtgttt atcacttccg catggggcct tggtgaaatg gtcgtgcagg
     3181 gcgcggttaa cccggatgag ttttacgtcc ataaaccgac acttgcagcg aatcgcccgg
     3241 ctatcgtgcg tcgcaccatg gggtcgaaaa aaatccgcat ggtttacgcg ccgacccagg
     3301 agcacggcaa gcaggttaaa atcgaagacg taccgcagga acagcgtgac atcttctcgc
     3361 tgaccaacga agaagtgcag gaactggcaa aacaggccgt acaaattgag aaacactacg
     3421 gtcgcccgat ggatattgag tgggcgaaag atggccacac cggcaaactg ttcattgtgc
     3481 aggcgcgtcc ggaaaccgtg cgctcacgcg gtcaggtcat ggagcgttat acgctgcatt
     3541 cacagggtaa gattatcgcc gaaggccgtg ctatcggtca tcgcatcggc gcgggtccgg
     3601 tgaaagtcat ccatgacatc agcgaaatga accgcatcga acctggcgac gtgctggtca
     3661 ctgacatgac cgacccggac tgggaaccga tcatgaagaa agcatctgcc atcgtcacca
     3721 accgtggcgg tcgtacctgt cacgcggcga tcatcgctcg tgaactgggc attccggcgg
     3781 tagtgggctg tggtgatgca acagaacgga tgaaagacgg tgagaacgtc actgtttctt
     3841 gtgccgaagg tgataccggt tacgtctatg cggagttgct ggaatttagc gtgaaaagct
     3901 ccagcgtaga aacgatgccg gatctgccgt tgaaagtgat gatgaacgtc ggtaacccgg
     3961 accgtgcttt cgacttcgcc tgcctgccga acgaaggcgt gggccttgcg cgtctggaat
     4021 ttatcatcaa ccgtatgatt ggcgtccacc cacgcgcact gcttgagttt gacgatcagg
     4081 aaccgcagtt gcaaaacgaa atccgcgaga tgatgaaagg ttttgattct ccgcgtgaat
     4141 tttacgttgg tcgtctgact gaagggatcg cgacgctggg tgccgcgttt tatccgaagc
     4201 gcgtcattgt ccgtctctct gattttaaat cgaacgaata tgccaacctg gtcggtggtg
     4261 agcgttacga gccagatgaa gagaacccga tgctcggctt ccgtggcgcg ggccgctatg
     4321 tttccgacag cttccgcgac tgcttcgcgc tggagtgtga agcagtgaaa cgagtgcgca
     4381 acgacatggg gctgaccaac gttgagatca tgatcccgtt cgtgcgtacc gtagatcagg
     4441 cgaaagcggt ggttgaagaa ctggcgcgtc aggggctgaa acgtggcgag aacgggctga
     4501 aaatcatcat gatgtgtgaa attccgtcca acgccttgct ggcagagcag ttcctcgaat
     4561 atttcgacgg cttctcaatt ggctcaaacg atatgacaca gctggcgctc ggtctggatc
     4621 gtgactccgg cgtggtgtct gaattgttcg atgagcgcaa cgatgcggtg aaagcattgc
     4681 tgtcgatggc tatccgtgcc gcgaagaaac agggcaaata tgtcgggatt tgcggtcagg
     4741 gtccgtccga ccatgaagac tttgccgcat ggttgatgga agaggggatc gatagcctgt
     4801 ctctgaatcc ggacaccgtg gtgcaaacct ggttaagcct ggctgaactg aagaaataaa
     4861 ataaatcccc ggcggcgttc agtcgccggg tttatgtgat ccccgaagat aaaacttatt
     4921 caatctcttc acagacatcc tgcgttaatc gccgcataat atcttttctt aacaaaaact
     4981 tttgtatttt acctgaggca gtgcgcggta gtttttcgat taccacgata tgttcaggat
     5041 atttatattt tgcgacccgt ttacggctaa aaaaagccac cacctcttcc agcgataatg
     5101 aatgatgcgg cgctttcagc acgacataag cgcatgatcg ttcacctaaa cgttcatcgg
     5161 gcattgcaac cacacaggca tcgtgaattt taggatgctg caataaaata tcttccactt
     5221 cacggctgct aatattttcg ccgccgcgga caataatatc ttttttgcgt ccggtaattt
     5281 ttatatagcc agcctcatcc atacggcaga gatcgccgct gtaataccag ccttcttcat
     5341 ccagcgcacg ggcggttaat tcaggttcat caaaatatcc cataaacaca ttggggccac
     5401 gcgaggcttc ttcaccttcg caacctggcg gtaaggtctt gcgtgcgtca tcgaccactt
     5461 taatctctac acctgcggca gcgtaaccat cggtgtgcat aaagcgcgac aaaggatcat
     5521 cgagattcac caccgcatgc ggcgaacttt ctgtggaacc ataaacactt aataatttaa
     5581 tgccgagctg ctggcattca cgcgccactt ttttggggat tgtggttccg ccgcaaagaa
     5641 agaaacgcag cgctgacagg tccgcgggtt gtttctctag tacattcaaa agatcataga
     5701 caaacggcgt tgcgcctagc atacaggtgc aacgctgctg ctcaagcagc gcgagacacg
     5761 catcaggagt gaaaatatct aacaacacgc tgcgagcgcc aattaagaat ggtgccgtta
     5821 cgccatgcag aaagcccgtt gcgtggccga gtggcgcagg catcataaag acatcctgcc
     5881 aggtcagatt cagccgcgcg caataagccc gctcactggc gagaatattg ttatgcgtta
     5941 gcatcacgcc ctttggcaga ccctcgattc cggaggtaaa cagcaccgca gctaattcat
     6001 cgccgtgggt cgttatcgcc gtggtcagtg gggtattgtc ggcgataatc tgacttaatg
     6061 agagggaaga ggtggcggga gccagtttgt ccacgccgac aatttgttgt agttgtggaa
     6121 gctgattttg cagcggcagg attaaatcta ccgggcgcgt ttgtttaaac aacgtcggtg
     6181 caaagaacat ttttgcctga cacttattga gcacccacac cagttctgct tcccgccagg
     6241 aaggcaacag cggcacggaa accgcaccga ttttcaggca ggcaagatag ataacggtaa
     6301 attcacacca gccaggcagt tgaaatgcga tgcgatcgcc tgattcaata cccttcgcta
     6361 acatccagtt tgccagacag ctcgcggcgt gatcgagcgc gctataggtg tacgatgcac
     6421 catgattatc gaccacggca attttgtctg gcatcgcacg agcggtctgc tgccagtaat
     6481 cggccagcga agcatcgccc catagccctt gctgacgata cgccgcacga cgttgttcgt
     6541 tgaacgttaa tgtcactttc atgttggact ctcgaaacag aacatcaggc ccgagatgcg
     6601 ggcctgtggg atgcatgatt agccgtagcg aaaatcaata ccgaaggtgc ctgcgggata
     6661 ttgccactgt tcgagaatag tgttaccgca cagcacccga caggtgccgc actccagaca
     6721 accggcagaa tcaaaatgaa tgttgccggc gtcatcctgc ttataaagtc cggcagggca
     6781 ggctttcatt aatttacgga attcattgat atcgggattt gccgccagaa tgatatgcgg
     6841 gtggccctca tcaacatgga atttattgac gcctaatttg atgtcgacgt taaccgtagc
     6901 gttctggctc atagcgcggt tgctccctta atgccatctt tcagcaagtt gatcagccca
     6961 attttcttcg catgtcccat gatcattttg cgtaccggct ggtttggttt gccgtcaatg
     7021 gtgaacatct cgttcatgat gtcggcgacc attcgtgggt attggctaaa caggcgcggg
     7081 ttttccatca gcgccgggat cttgcggaaa tgctgcatat cgcgcatgac gcagctttgt
     7141 tccagctcac gtttgtattg cgccagactg ctggcggaga aatcctcgcg ttctttggcg
     7201 gcgatcaccg ttgtggcggc agcctgagcc gatgcaatgg ctaaatccat gccacgaact
     7261 gtaaaaccca aattcaggca gaagcctgcg gcgtcaccaa cgatcatcac gccatcgtta
     7321 accatctgcg gcaccattgc cagaccgcct tctggcacca tatgcgcgga atattcaagc
     7381 agtttgctgc cgctaattag tgggcgaatg gcggggtgtt gtttaaaatc ttccagcatt
     7441 tgcggcacgc ttttttgcgc atgggcgata tcacccaatc cacaaaccag ccccaaggat
     7501 atggaatcct tgttggtata gagaaatccc ccgcccatca ggccgtcaga aggggaaccg
     7561 gcaaacagcc aggcggcacc ttcctcgccc gtaatattaa agcgatcgtt gatctgttct
     7621 ggtgtgaggc caataacctc tttaacacca acagcgtaat gatgcggatc ggaagcggga
     7681 accattccca gcgagcggcc aagcatcgag ttaacgccat cagccagaat caccacattc
     7741 gcttcgagaa tatcatcccc agcctgcacg ccagtgacct tgtttccttc acgaaccagc
     7801 gcatcgacgc gaacgcctgg gataaactgc gcgcccgcct gctcggcttg ttccatcaac
     7861 cacgggtcca gacgattacg caatacggta taagatgcgt gttgcggaac atctggttgc
     7921 tcgcggtgaa aatcgagggt aacggcgctt tcttcggtta agaaggagat tttctcgcgt
     7981 gtgaccttgc gttctaccgg cgctgatact gcaaagcctg gaatgattgc ttcaagtgtg
     8041 tgggcataaa gacgcccgcc ggtcatgttt ttacatccgg cactgtcgcc gcgttctatc
     8101 accaggacat ccagcccggc tcgcgccatg accagtgcgg caacgctacc agcaacgccc
     8161 gcaccgacca caatggcatc aaatttgtct tccgacatgc gttatgctcc cttcccgctg
     8221 cgtttacagc gggagatgtg gttgaaaggg gaattagcgg ctcaactggc taatcagggc
     8281 agggacgact ttgtagatat cgcccaccag accgtagtcg gcataattga agattggcgc
     8341 atttttatct ttattgatgg cgacaatcac ttttgcgccg ttgccgccaa ccatatgctg
     8401 gatctgcccg gagatcccca gcgtcaggta gagatcggat ttcagcaaca cgccggagac
     8461 accgatataa cgttcacgct ccatccagtt ctcgccttcg gcaattggac gtgaacagcc
     8521 gacttcagca ttcagcaccg ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc
     8581 cagaccacga ccgacgccaa ccacacgttt tgctttgctc aggtccacgc tgcttgcggc
     8641 tttcgcacgg cgttcgcgac agagaatttc atgacgcgga gcaacataag gtaccgtttc
     8701 tgtcgggcac tgatgagagg tatcactggt gcacggttcc tgaacaccgg gtgcaagggt
     8761 aatgatcgcc agcgggctgt tgatcttttc ctgagcgaac gctaacccgc catacatccg
     8821 gtgttcggcg caaatgtgac catcgacaat atccaccgcc gtggcatcgt tcaccagcgc
     8881 cgcattcagt tgcacactta accgtgctgc cagagcttta ccacgtttcg tcgcggccaa
     8941 cagcaacata gcggggtgtt tatctttcag cagggcagca atgctttcgg cgtaattttc
     9001 agtgcgttgc agcgcgtcgt tttgctcaag aacataaata cattttggac cataaggcat
     9061 aactgcctgc gcctggtcgg tattttgtac aatggcatac acctgttggc cccattgctg
     9121 agcgccgcca aacagttcag cataacgttc aggattatcg ctaaagaccc agacgctgtt
     9181 taattgactc atcgtttctt ctccgtaggc ttcagttcag ggctttcttc agatgttccg
     9241 ccagctcggc aatggcctcc ggcgaatcgt tatcgataat gatgtgctta cgttctgttt
     9301 gcggcggtac gcgaatgccg accagttcag caagtggcgc gctctggctc cagtcaatat
     9361 cacttgcctg ccactgattt accggttttt tacccgcgcc gagaatggct ttcatcgaag
     9421 gaatgcgtgg cacgttaata tcggaggtga cgcagagcac ggctggaaca gagagttcaa
     9481 taacttcaat atcatcttca agcgtgcgtt caatcaccag tgtattgccc tgacgctgaa
     9541 tagcactcac tgcattaatc accggaagtt gcagaatttc tccgaccagc aagccaacct
     9601 gctgggcata aaggtcgccg gaaccttcac caaagatcag taaatcgaag ccgatctttt
     9661 caattgctgc cgccagcgct tttgcggtat cgagaggcag tgcatgttca agttgcgcat
     9721 cctgcaccaa atacaggctg tgcggcccgc gggatagcac gtctttgcgc actttcgagt
     9781 tctgcaacaa tgagccgcca acggtcagcg cggctatctc atcgtcatct gttgcgagct
     9841 ggcttgcagc ttcaatggca ttgagatcga actggctgat tttggcgtcg gcattgtcga
     9901 aattcagggt gtattctgga gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc
     9961 aggttattat tttcatgatt tctcctgtga gtcgcatacg gaaccatctc cgatggaagg
    10021 taaaaatggc ctgaaaatag tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt
    10081 tttccttgcc aacaatcttg caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg
    10141 gcaatatagt tattccatga ccgtacaatc ttgcattcag tgtaaataat cttgtttagc
    10201 tctcgctaaa cgcggttgta taaccgcaat acacgccgta ttttatccgg catatattgt
    10261 ttttgcttgc tcatggaata gggaggttgt catgtatcaa cgctgttttg ataacgccag
    10321 tgaaacgctg tttgtcgccg gtaaaacgcc acggctttca cgttttgcat ttagcgatga
    10381 tccaaaatgg gagtccggac atcacgttca tgacaatgaa accgagttga tttacgtcaa
    10441 gaaaggggtt gcaaggttta ccatcgattc ttcgttatat gtcgcacatg cggatgacat
    10501 tgtggtgata gaacgcggca ggctacatgc ggtggcctct gacgttaacg atccggcaac
    10561 gacgtgtacc tgtgcgctgt acggctttca gtttcagggg gctgaggaaa atcagctact
    10621 gcaaccgcat tcttgtccgg taattgccgc ggggcaggga aaagaagtca taaaaacctt
    10681 atttaatgag ctaagtgtga ttttgccgca aagtaaaaat agccaaacat cttcgttatg
    10741 ggatgcattt gcctatacgt tagccattct ttactacgaa aattttaaaa atgcttatcg
    10801 ttcggagcag g
//
LOCUS       DAZGTR010000142        10777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGTR010000142 DAZGTR010000000
VERSION     DAZGTR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.144"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(364..1557)
                     /gene="tyrB"
                     /locus_tag="ACHLCY_004121"
     CDS             complement(364..1557)
                     /gene="tyrB"
                     /locus_tag="ACHLCY_004121"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP0371272.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKSRELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1810..2889)
                     /gene="alr"
                     /locus_tag="ACHLCY_004122"
     CDS             complement(1810..2889)
                     /gene="alr"
                     /locus_tag="ACHLCY_004122"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP0371273.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2942..4357)
                     /gene="dnaB"
                     /locus_tag="ACHLCY_004123"
     CDS             complement(2942..4357)
                     /gene="dnaB"
                     /locus_tag="ACHLCY_004123"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0371274.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4440..5423
                     /gene="qorA"
                     /locus_tag="ACHLCY_004124"
     CDS             4440..5423
                     /gene="qorA"
                     /locus_tag="ACHLCY_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP0371275.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5589..5831)
                     /gene="pspG"
                     /locus_tag="ACHLCY_004125"
     CDS             complement(5589..5831)
                     /gene="pspG"
                     /locus_tag="ACHLCY_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP0371276.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5965..7002)
                     /gene="dusA"
                     /locus_tag="ACHLCY_004126"
     CDS             complement(5965..7002)
                     /gene="dusA"
                     /locus_tag="ACHLCY_004126"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP0371277.1"
                     /translation="MHDNHEAQKINQTSVMPEKTGVYWNSRFSIAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNTQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            7091..8189
                     /locus_tag="ACHLCY_004127"
                     /pseudo
     CDS             7091..8189
                     /locus_tag="ACHLCY_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            8251..8499
                     /locus_tag="ACHLCY_004128"
     CDS             8251..8499
                     /locus_tag="ACHLCY_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0371278.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWKSADEWFVH"
     gene            complement(8615..8767)
                     /locus_tag="ACHLCY_004129"
                     /pseudo
     CDS             complement(8615..8767)
                     /locus_tag="ACHLCY_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(9001..9591)
                     /gene="espM2"
                     /locus_tag="ACHLCY_004130"
     CDS             complement(9001..9591)
                     /gene="espM2"
                     /locus_tag="ACHLCY_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM2"
                     /protein_id="HFP0371279.1"
                     /translation="MPMNTTGMSFSSFGISCHKENSFRNSFRGKNDEVVKCSIGERTI
                     SFSVRKFSGNILETVRRQSTKDIDEWIKDERIVYPSRVINQEIDNYYFQKNAKISTEE
                     RQRVFFLVSQENQLTLDVKAAQSSINHVIMGSASFGKKMDALCDGMSRDVKNRTSDTI
                     ANLLADKFYQKHIDSDIDIVKLRNDIPDYLMRAIQG"
     gene            9774..10424
                     /locus_tag="ACHLCY_004131"
     CDS             9774..10424
                     /locus_tag="ACHLCY_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0371280.1"
                     /translation="MPGILNFSNGSVLPENELEALRHIARNNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADRLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            10503..>10777
                     /gene="espW"
                     /locus_tag="ACHLCY_004132"
     CDS             10503..>10777
                     /gene="espW"
                     /locus_tag="ACHLCY_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP0371281.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNPVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAIN"
BASE COUNT         2952 a         2647 c         2465 g         2713 t
ORIGIN      
        1 aagcggaaaa aaagagctct ctttaacttt agaatttaac gatactataa ttgaatctca
       61 gaaagtcaca ggcaataagg caaagcatat tcttgaggat atagaaatat tctatcgtaa
      121 caaacccgat acttattatt aaatttaata atggttagat gtgcagaaat tattactcca
      181 cccaaaaaaa caggataagg aattccagat aattgggata agcttaacaa taaaagaact
      241 aacaacgttt attatctctc acgtagaacg atggcatcaa aaaaatcatc tataaagaaa
      301 gagtgaaagc gacgacagtt taatttcact gcaggctggg tagctccagc ctgctttcct
      361 gcattacatc accgcagcaa acgcctttgc cacacgttgt acatttgccg tatttaaccc
      421 ggcgacacac atgcgaccgc tggcgatgag atagacacca aattcttcac gtagtcggtc
      481 aacctgagcg gcacttaaac cggtataact gaacatgccg cgctgattaa gcagataatc
      541 gaaattgcgt tctggcatct ctgtgcttaa taccttcacc agttcctgac gcattgccag
      601 aatgcgagta cgcatctctt ctacttccgc cagccagctg gctttcaatg cctcgtcatt
      661 cagcactgca gccaccacct gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac
      721 tgttgctttc aattgcccca gtacgcggcc agcggcttcg gcatcttcac acagaacaga
      781 aagtccgccg acgcgctcgc cgtaaaggga gaaaattttc gagaacgaat tgctcaccag
      841 agcgggtaat ccagcgctgg caatggcgcg aatagcgtag gcatcctctt ccataccggc
      901 accaaatcct tgataggcaa tatcgaggaa tggaataagc tcgcgggatt tgagaatttc
      961 aatcaccgca tcccactgat cattagtgag atcggcaccc gttgggttgt ggcaacatgg
     1021 atgcagcaac acaatactgc gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa
     1081 gcgcacgccg ttagtcgctt cgtcatacca ggggtaagta ctcacttcga atccagcccc
     1141 ggcgaatatt gctacgtggt tttcccaggt aggatcgctg acccagacgc ctgattccgg
     1201 gaagtagcgt ttcaggaaat ccgcgcccac tttcaatgcc cctgagccgc caagggtttg
     1261 aatggttgct acgcgctgtt gttgcagtac tggatggtcc gcaccaaaca gcagcggcgc
     1321 aatggcatgg cgatagctgt taagcccttc catcggtaaa taaagcgaag cgccatgagg
     1381 ctgcgcattc aggcgcgctt ccgcctccgc cacggcttgc agttgtggaa taattccgtc
     1441 ttcgttgtag tacagaccga tacttaaatt cactttgtcg ctgcgagggt cttctttaaa
     1501 acgctccata agcgtaagaa tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg
     1561 atggttctcc aggtttacgg gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga
     1621 tcgagtggat gttcaggagc gaacggcaat tagcaacaga gtgagactca tgacaaacgt
     1681 acatccgcca gaggcacgac ctttataaga gcatgaaaga acatcaactt attgaatttt
     1741 taggatttta ttggccggat aaggcattca cgccgcatcc ggcacagaca atcaaatatt
     1801 acagaacgat taatccacgt atttcatcgc gacccttgaa gtcaggcgcg taataagttc
     1861 gtaagcgctt actttcgtca tttcagcgat acgttctacg ggcaaacctt cgccccataa
     1921 aatgaccgga tccccggctt tgtcctgcgc ttgtggacct aagtctacgc agatcatatc
     1981 catcgccacg cgcccgacaa tcggtacttc gcgaccgttc accagcactg gcgtaccgga
     2041 cggcgcggcg cgcggataac catcgccata gcccatcgcg actacgccaa gacgggtatc
     2101 acgttcgctt acccaggttc caccataacc aacaggttct ccggctttat gctcacgcac
     2161 ggcaatcagg ctggaggtta gtgacatcac tggctgacag ccaaaatcgg caccggtgga
     2221 gcgatcttcc agcggcgaga cgccataaag aatgatgccc gggcgcaccc agtcaaaatg
     2281 cgactgtggc cacagcagaa tgccacccga tgcggcaatg gaacgttgac caggtttgcc
     2341 ttcgcaaaag gtattaaaga tagcgagttg tttctcggtt gcgccacatt ttggttcatc
     2401 cgcgcgcgca aaatggctga cgatattcac cggctgacga acgtttttgc actgggtcag
     2461 gcgatgataa aacgcctcag cctgttccgg ccttacgccc agacggtgca taccggtatc
     2521 gagtttcatc cagacggtaa ccggctcgtc caggctagcc tcttccagcg cagccagctg
     2581 ttcttcgtta tgcacggcgg tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc
     2641 atcaaaaaag ccttcgagta acagtacagg tttggtgatt ccccccgcac gcagtcgcag
     2701 agcttcttcg agacgggcta cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc
     2761 aagaagaccg tgaccataag cgttcgcttt caccaccgca accattttac tggcaggggc
     2821 cagttcacga agacgttgca ggttgtgtcg cagagcgcgg cggttaatca caacagttgc
     2881 cgcttgcatt tgtgctcctt gataagtgtt tgctttaatt acctaattca taaaataatt
     2941 attattcgtc gtcgtactgc ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa
     3001 aggtcaggcg taccgtcccg attgggccgt tacgttgttt accgataata atttccgcga
     3061 tgccttttaa atcactgttt tcgtgataca cctcatcacg atagataaac atgatcaagt
     3121 ccgcatcctg ctcgatagag ccagattcac gcaggtcgga gttgaccggg cgtttgtcgg
     3181 cacgttgttc cagagaacgg ttcaactggg acagcgccac caccggcacg ttcagttctt
     3241 tcgccagtgc tttcagcgag cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa
     3301 gcgccggtac gcgcatcagt tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt
     3361 cacgggcaat acggcgtgcg cgggaacgca cttccgttgg cgtcaggccg gaggagtcat
     3421 cgatatagat attgcgtttt tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt
     3481 cttcatcatc gagctgcccg gtacggattt tagtctggtc aacgcgcgac agcgacgcca
     3541 gagaacgcat cataatctgt tctgaaggca tctccagcga gaagataagt actggtttat
     3601 cctgcaacat cgccgcgttt tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg
     3661 gacgcgcggc gacgatgatc aaatccgacg gctgcaagcc agcggttttt ttgttgagat
     3721 cgtcataacc ggtgtttact ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa
     3781 tacgcgccac ggttgcgtcg agcacatcgg cgatgttctt cggcccttcg tctttgtttg
     3841 cacgactttc ggcaatttta aagacgcggg attcagcaag gtccagcaga tcttcgctgg
     3901 tacgcccctg cggatcaaaa ccggcttcag caatctcatt cgcaaccgag atcatctcac
     3961 ggacaacggc acgttcacgc acgatgtcag cataagcact gatgttcgcc gcacttggcg
     4021 tattttttga cagctctgcc agataagcaa aaccaccgac gctatcgagt tgcccctggc
     4081 gttccagcga ttccgcaagg gtaatcagat cgataggact accgctttcc tgcaaacgcg
     4141 ccatttcagt aaagatatgg cggtgtgggc gggtgtaaaa atcgtctgcc accacacgct
     4201 cggctacatc gtcccagcgt tcgttatcca gcattaaacc gcccaacacc gactgctccg
     4261 cttcgatcga gtgcggaggc actttcagcc cggcaacttg tggatcgcgt tcgcggggtt
     4321 cagcctgctg tttgttgaag ggtttatttc ctgccatagt gaatggagtt accgagataa
     4381 agaatgggtc gaaactttac catatgaagc agcccctgac gatacgttct ggaggacaca
     4441 tggcaacacg aattgaattt cacaagcacg gtggcccgga agtacttcaa gccgtagagt
     4501 tcactcctgc cgatccggca gagaatgaaa tccaggtcga aaataaagcc atcggcatca
     4561 attttatcga cacgtatatc cgcagcggcc tttacccgcc gccatcacta cccagcggat
     4621 taggtaccga agcggcaggc atcgtgagta aagtcggcag tggtgtaaag catattaagg
     4681 caggcgatcg tgttgtctat gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca
     4741 ttaatgcgga taaagcggcg attctgcctg cggcaatttc ttttgagcaa gctgcggcat
     4801 ccttcctgaa aggcttaacg gtttattatc tgctgcgtaa aacctatgaa attaaacccg
     4861 atgaacagtt cctgttccac gcagcggctg gcggcgttgg cttgattgcc tgccagtggg
     4921 caaaagccct gggcgcgaaa cttatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg
     4981 cgctaaaagc gggcgcgtgg caggtaatta actatcgtga agagaatctg gtcgagcggt
     5041 taaaagagat caccggcggt aagaaagtgc gcgtggtgta cgattccgtg ggcagagaca
     5101 cctgggaacg gtcgctggat tgcctgcaac gccgcggctt aatggtcagt tttggcaact
     5161 catcaggtgc ggttaccggt gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg
     5221 tgacacgccc ttccctgcaa ggctatatca ccacgcggga ggaattaacc gaggccagta
     5281 atgaactgtt ctctttgatt gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga
     5341 aatatccgct gaaggatgcg cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag
     5401 gttccagcct gctgattcca taaaagaaat agggcttcca cctgggaagc cctttctttt
     5461 tatagttcgg ctgtatgtag ggtacagcac gatgaatctg ttagaggcgc aatagtgaca
     5521 gatttgatta tcaattccta ttttgttcta aggataaaac cttaggttgt gatcatccgc
     5581 acaatccctt agtaacgcca gcggtcataa cgctgatatt taggcacttt tggtgcttta
     5641 atcgccttaa taacccacac caccgcaatc gccagtagta accacggcag caacttaatc
     5701 atcaatgcca gcataccgcc gaggaacata atggccgtcg ccacaaccag cgcggcgata
     5761 atgcccagca acgaaacgcc ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt
     5821 agttccagca tgatgctctc ccaaatatga aatctcttgc tggcattaca agaatcatgc
     5881 caaaaataat ctattgattt aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga
     5941 atttgactac tttttggtga aaagttaacg cttatccgcc accagtttga gcgcgtgttc
     6001 cagcacatta atgtctgcac ccgctttatg ggcattttca cttaaataac gccgccactg
     6061 ccgcgcgcca ggaataccct ggaacaagcc caacatatgc cgggtaatat ggccgagata
     6121 cgtcccctgg ctgagttcac gctcaatgta cggatacatg gcgcgcacta ccgccaccgg
     6181 atcggcatcg gtatccgagg aaccaaagat ctcccggtct accgccgcca gaatacccgg
     6241 attctgatac gcctcgcgcc cgaccatcac gccatccata tgttgcaggt gtgctttggc
     6301 ctcttccagc gacttgatac caccgttaat cgacattgtc agatgcggaa agtcacgctt
     6361 cagttgatac acacgcggat aatcgagcgg cgggatttca cggttttctt tcggacttaa
     6421 ccccgaaagc caggctttac gtgcgtggat gataaacatc tcacactcgc ctttgccgga
     6481 aacggtgttg atgaaatcgc agagaaattc atagctgtcc tggtcatcga tgccaatacg
     6541 cgttttcacc gtcactggaa tcgacaccac atcgcgcatc gctttcacgc agtcggcaac
     6601 cagctgcgta ttacccatca gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg
     6661 gcagccgaca ttcaggttga tctcatcata tccacgcgct tctgccagct tcgcacactg
     6721 tgccagcgcc gccggatcgc taccgccgag ttgcaacgct accggatgtt cttcttcact
     6781 gtacgccagg taatcacctt taccgtgaat aatcgcccct gtggtcacca tttcggtata
     6841 cagcaacgta ttgcgggaaa gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc
     6901 gagcatagga gcaatgctaa accgagaatt ccagtaaaca ccagtttttt caggcatcac
     6961 gctggtttga ttaatttttt gtgcttcatg attatcgtgc atttttgaac atttcaggct
     7021 atttttctcg cgttaggttc ccgcacaggt tcccacgttt tatgggaacc cgaaataacg
     7081 aggtcgtgta atggcgtact ataacataga gaaacgacta aaatccgatg gcacaccacg
     7141 ctatcgctgt aatgtgatta tcaaagaaaa aggtgttatc acttacaggg aaagcaaaac
     7201 attccctaaa catgctcatg ccaaaacatg gggcacacag aaagtgatgg aattagatct
     7261 atatggcatt ccatcatcaa atgcagttga cggacttaca gtccgtgact tactacacaa
     7321 atatttaaat gacccaaatg ccggaggtaa agcaggccgt actaaaagat atgtgctgga
     7381 actgcttatg gatagtgaca tatccgcgat caaactatct gaactgacag aaaatgacgt
     7441 aattgaacat tgcaggctaa gaaacaacgc tggtgcaggc ccagcaacag tcagccacga
     7501 tgttagttat cttggcagtg ttctggatgc ggcaaaacct gtatacggaa tcaattacac
     7561 atcaaacccg gcgaaaagcg ctcgtccata tctacttaaa ctcggtttga ttggtaaatc
     7621 aaaccgtcgt aatcgtagac cagcatctga tgaacttgac atgctcattg aaggccttca
     7681 acaacgatct actcataaat gctcaaaaat tccgttcgtt gatatcctca aattttctgt
     7741 gtggtcctgt atgcgaatcg gagaagtatg ccggttacga tgggaagatc tcgaccaaga
     7801 acaaaaatct atactagtaa gagataggaa agatccacgt aaaaaggaag gttgaccata
     7861 tgaaagttgc cttgcttggg gaagcctggg atatcgtcca gcgacaacca aaaaaatcag
     7921 aattcatttt tccatataac agcacttctg ttaccgcagg atttcagagg gtaagaagca
     7981 aattaggtat taaagatctg cgatatcatg atttgcgtag agaaggggca agtcgcttat
     8041 ttgaggctgg ttttagtatt gaggaagtcg ctcaagttac agggcatcgt tcattaaacg
     8101 tgctatggca ggtatatacc gaactgtatc cgaaatcttt acataatcgt tttgaagaac
     8161 tccaaaagag cagaaacaag acctcttgac actgtttatt tatacagata aaaataatac
     8221 tgtatacaaa cacagtatag agggattttt atgcgtattg aaatctgcat agccaaagag
     8281 aaaatgacta aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc
     8341 atcagtaaac gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt
     8401 tctgttacgc gcaccgccga taaagattca gctaaaactt ttgttcagga gactctgaaa
     8461 gatacctgga agtctgctga cgagtggttt gttcactaat taacacgtaa aatcggtaac
     8521 ggctggaaat cattcaatac tcgcactatc gaaagttcac cagccaaccg cagcacgtcc
     8581 tgcatacgtc gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc
     8641 actcaatatc aggtgcagtt gatgtatcaa cacggttcag caacacccga tactttttct
     8701 aggcttccag caatgaggtt tcttcctccg ttgcaatttc cagatctgca gcatcctgaa
     8761 gtggggcgca ccacgcaaac atcacaaaat attgtcgctg cgatgaacct gtacaataaa
     8821 taatgaatca cggtacgttt ctgtgtataa cgtactgtag ttttctttac gatttatagc
     8881 tgtactggtg agccatgagt aatctgaatc catgcatggc gtgtggtgcc gttgtgcatt
     8941 ttctgcgtct cttttactgg tccgaagccg atgatgctga agcagtcctc tcctctagcc
     9001 tcatccctgt atagcacgca tcaaataatc tgggatatca tttcgtagtt ttacaatatc
     9061 aatatccgaa tcgatatgtt tctggtaaaa cttgtccgca agcaagtttg ctatggtatc
     9121 tgatgtacga ttttttacat cccggctcat accatcacaa agagcatcca ttttcttgcc
     9181 aaaagaagca ctccccatta ttacatgatt aatagaactt tgtgctgcct taacatcaag
     9241 ggttagttga ttctcctggc tcacaagaaa aaagactctt tgcctctctt cagtggaaat
     9301 ttttgcattt ttttgaaaat agtaattatc aatctcctgg ttgatcaccc ttgagggata
     9361 tactatccgt tcatctttta tccactcatc aatatcttta gtactctgcc ttctcaccgt
     9421 ttccagtata ttgccgctaa atttgcgaac agaaaagcta atagtccgtt ctccaattga
     9481 acattttacg acctcatcat tcttccctcg gaaagagttc ctgaaggagt tctctttatg
     9541 gcaacttata ccaaaagagc tgaaagacat acctgtagta ttcatcggca ttataatgcc
     9601 tttttataaa aaagtgttta caatattgca tacttgcgtt tattgtagcc agaaaaaaca
     9661 tgcatgcaat aatattctat taaaaattgt attgttttat tcacatatca tgagcggtat
     9721 gttgattctt gttaccaata tagttcacaa tatggattaa aaaggataat aatatgccag
     9781 gcatattaaa tttttctaat ggaagtgtat tgccggaaaa tgagctggag gctttacgac
     9841 atatagcaag aaacaaccag aatgacacta ttactatagg aggccgtaat atgaggcttc
     9901 attatatcca gtttatggat ggttttagtg ttgagcctat tcttggtgga ctttgggacc
     9961 atcttggggc aagagaggct catcatctcg cagaccgcct gacaagacaa cttaacggag
    10021 gtaacacttt tctgcaggca tacagcttat atctggagca gaggcaagct gccccgcttg
    10081 tgcaggaaag cgtcataaaa acactactag atcgaataaa ttcgaatgca tttcccgtta
    10141 gtttacaaga cttttcctgt actgaggaac atcttaattg tccgataacg ctacatattc
    10201 ctgagacagg tgtttttgtc agaaatgctc gaaattcaga aatatgtgca ttatatgatc
    10261 aggaagcatt gactgaactt atcctgcgta acgctctcca ccccctcagc cgtgacccct
    10321 ttgctccaga aatgattata agcaaagaca agtgtcattt taatataaca aaacaatgtt
    10381 tttacgcatt acccatatac ccacttcaac aaaacagtat ttaaaaataa aagcacaata
    10441 taagaagtat attttcaata agttaatcac catataacaa acatatagcg aggagactat
    10501 ttatgcccaa aatatcatca gttgtatcat catgttacca tctgttcagt gaacatcaac
    10561 aactttcaaa tgaaacaaca atgacgaacc ccgtctccag aagaattgtt cataaagaat
    10621 atggtatatc tttaaaatcc gttcctgtat ggttggctac agctaaaact ccccttgctc
    10681 tactcaatgg cagacataca agaagtcact catttattat tgcagggacc ccaggaatgg
    10741 gaagcaggag cggagcccaa tactatgcca taaacag
//
LOCUS       DAZGTR010000143        10705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGTR010000143 DAZGTR010000000
VERSION     DAZGTR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.145"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(216..1898)
                     /gene="glcA"
                     /locus_tag="ACHLCY_004133"
     CDS             complement(216..1898)
                     /gene="glcA"
                     /locus_tag="ACHLCY_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP0371282.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2254..4425)
                     /gene="glcB"
                     /locus_tag="ACHLCY_004134"
     CDS             complement(2254..4425)
                     /gene="glcB"
                     /locus_tag="ACHLCY_004134"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP0371283.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPECVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFQVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDAAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4447..4851)
                     /locus_tag="ACHLCY_004135"
     CDS             complement(4447..4851)
                     /locus_tag="ACHLCY_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP0371284.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4856..6079)
                     /gene="glcF"
                     /locus_tag="ACHLCY_004136"
     CDS             complement(4856..6079)
                     /gene="glcF"
                     /locus_tag="ACHLCY_004136"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP0371285.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6090..7142)
                     /gene="glcE"
                     /locus_tag="ACHLCY_004137"
     CDS             complement(6090..7142)
                     /gene="glcE"
                     /locus_tag="ACHLCY_004137"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP0371286.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7142..8641)
                     /gene="glcD"
                     /locus_tag="ACHLCY_004138"
     CDS             complement(7142..8641)
                     /gene="glcD"
                     /locus_tag="ACHLCY_004138"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP0371287.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            8892..9656
                     /gene="glcC"
                     /locus_tag="ACHLCY_004139"
     CDS             8892..9656
                     /gene="glcC"
                     /locus_tag="ACHLCY_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP0371288.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9663..>10705)
                     /gene="yghO"
                     /locus_tag="ACHLCY_004140"
     CDS             complement(9663..>10705)
                     /gene="yghO"
                     /locus_tag="ACHLCY_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP0371289.1"
                     /translation="NWIPPLFIERNEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQ
                     IDTLHRERYGEDTGHFGMIDAIDDPQVFAALFGAAEAWLKSQGASKISGPFSLNINQE
                     SGLLIEGFDTPPCAMMPHGKPWYAAHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLM
                     DQVRKKVTIRCINRQRFAEEMQILREIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVP
                     DDMIYIAEIDSAPCAFIVGLPNINEAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPL
                     MGVRDEYQFSRIGPVIALLLIEALRDPFARRKIDALEMSWILETNTGMNNMLERIGAE
                     PYKRYRLYEKQI"
BASE COUNT         2491 a         3020 c         2707 g         2487 t
ORIGIN      
        1 acggtgtttg ctcatcaagc gaacataatt aatacagact tgttttgcgt gataaaaaaa
       61 atagatgttc tcacgctctt aattatttat caggttatct gaacacaaaa cattattgcc
      121 gcatggataa taaagcgaga aataattttc aattaataag acagaataat gtaaatgaag
      181 ccggatgata ttaacgatca tccggcatta ttgatttacg agaccaacat tccagtaaac
      241 acatacgcct gcaacaacgt gataacgccg ataacgctgg caaaaatcag actgtgcttc
      301 acggtgtagc ggaatagttc agattctcgg cccaccatgc ccgtcgcggc acaggccacg
      361 gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt tgctgccacc
      421 agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga accaaacagg
      481 gcgtttgaag aagtgtccga gccggtaagg aataccccca gccagccgag gaacggtgag
      541 aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg
      601 gaatagttgg tgacgaaggc gaacgccagc accatgccaa tcgacaatat cggccacttc
      661 agactaatta gcgtttcggc aaagacgcca atccctttct tgatgcccac gccgaggatg
      721 aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag agggtcgaat
      781 ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt caacacttgt
      841 tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc gcccggagca
      901 aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa gattaaaaac
      961 ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg accgccagaa
     1021 gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc cgtttcggta
     1081 tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac cagcgccgaa
     1141 gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt gacagcgaag
     1201 cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca gccgtccatc
     1261 attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg tcccgccatt
     1321 gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat cggcacgccc
     1381 aacgcaccaa acgccactgg cgcagtgttg gcaatcagac acagccccgc cgcgtataac
     1441 ggtttgaagc ccaggcccac cagcagcgca ccggtaatcg ccaccggcgc accaaagcca
     1501 gccgctcctt ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga
     1561 tcgtcggtga tggagataac cgagctgcgg ataatatcga actgaccgct ggcaacggtt
     1621 aatttataga ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata
     1681 aagccatagc ctgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca
     1741 atcaggatag ataagataag ggttattgct ccagcgacat gtcctttcag acgtaatacc
     1801 gcgagtgcaa cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt
     1861 cctcccatcg gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct
     1921 ttatatgctt gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata
     1981 ggcgttatac tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa
     2041 gtatatcggg taagggtttc tgttctgcac acgcaggcgc agagtatcgt taagatgtcc
     2101 atattgttgt tataggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg
     2161 ataatcgctc cggttatttc cgggataaat gtactaccgc agttactatc atagccccga
     2221 caataaaact tgccggggct ttttttgacg ctattaatga ctttcttttt cgcgtaaacg
     2281 ccaggcgtgt aataacggtt cggtatagcc gtttggctgt ttcacgccga ggaagattaa
     2341 atcgctggca gctttaaaag cacacgagtt agcgaaattc cccgccatcg gacgataagc
     2401 cggatcgtca gcgttttgct gatcaaccac tttcgccata ttctccagcg acgcctgcac
     2461 ctgttctttg gtcagaatac cgtgacgtaa ccagttggcg atatgctggc tggagatacg
     2521 cagcgttgcg cggtcttcca tcagcgccac attgtgaata tccggcactt ttgaacaacc
     2581 aatcccctgc tccacccagc gcaccacgta ccccagaatc ccctgcacgt tgttatccag
     2641 ctcttgttgg atctcttgcg ccgaccagtt agcgttttca gcaaccggaa tagtcagcag
     2701 atcgtccagc agcggttcaa attcagcatt gaactcagtc tgggcaatgt tggcttgcac
     2761 gctctgtacg ttggtttggt ggtagtgcat cgcatggagc gtagcggcag ttggcgacgg
     2821 aacccaggct gtgtttgccc cggcacgcag ttggtcgccc ttctggctgt acatgtctgc
     2881 catcaggtcc ggcattgccc acatgccttt accgatttgc gctttaccgc gcagcccaca
     2941 gaacaggccg gaaagcacgt tattacgctc gtaggctttg atccacggcg tcgatttcat
     3001 ctgattttta cgcagcatcg ggccagcttc catcaccgaa tgcatttcat cgccggtacg
     3061 gtcgaggaaa ccggtattga tgaacgccac gcggttgcgt gcctgagcga tacagctacg
     3121 caagttcagc gaggtccgac gttcttcatc cataatgccc attttcaggg tattcggtgc
     3181 cataccgagc attgtctcaa tgcgggtaaa cagtttgttg gcgaacgcca cttcctgcgg
     3241 accgtgcatt ttcggtttca caatatagac gctgccagtg cgcgagtttt tctgcacttt
     3301 taaatcatag agggcaatcg cgccagtcat gacgccatca agaatgcctt ccgggatttc
     3361 attgccttcg ctgtcccaaa tcacaggaat ggtcatcaaa tgacccacgt tgcggataaa
     3421 cagcagcgag cgtccgtgca gagaaatttc agagccatcg gcggcggtgt actgacgatc
     3481 gtcattcagt ttacgcacga tttgtcgacc gtttttctcc attttctctt ggagagtccc
     3541 ctgcatcagg cccagcagat tgcggtacag caggatttta tcttccgcat caaccgccgc
     3601 gaccgaatct tcgcagtcga gaatggtact gattgcagct tcgacgataa catcgttgat
     3661 gtgcgccgca tcgtctttcc caatccgtcc gttggcatca atttgcagtt caatatgcag
     3721 gccgttattt ttcagcaaaa tgcaggtcgg cgcagcggca tcgccacggt aaccgacaaa
     3781 ctgcgccggg gtacgtaacg tggtttcttt accatttttc aactggatgc gtaattgttt
     3841 atcgaccacc tgaaacgcca ccacatcctg atagctgccg ttttccagcg gtagagattc
     3901 atcgaggaaa cgccgaaccc aggcgataac ctgctcaccg cgctgcggat cgtagccgct
     3961 gaccatcgcc ccttcctgcg ggatgatgtc gctgccgtat aacgcatcgt acagtgagcc
     4021 ccagcgagcg ttcgccgcgt tcagcgcgta gcgggcgttc attgccggaa ccaccagctg
     4081 tggccctgcc tgactggtga tttcgctgtc aatgcccgtg gtttccaccg tcacgcactc
     4141 cggttgcggc accaggtagc ccagttcacg caggaaagat ttataggccg ctttatcttt
     4201 taccggcccc ggattgctgc gatgccactc atcaagcgct gcctgaatgc gatcgcgttc
     4261 tgccagcaac tgacgatttt ctggtgccag atcgtgaacg atctcatcaa aattgcgcca
     4321 gaacgccgca gcgtccagcc ctgttcccgg taaaacttct tcatccacaa aacgtttaaa
     4381 attggcgtca atgcgtaaac ggccctgggt tatggtttga ctcattgttt ctctcctcgt
     4441 tttcgcttat ttcgccaaca ccgctgctgc cgctttcgcg acctgcgcat cctgtgctcc
     4501 ggttaaacca gaaacgccca cggcaccaat aatttgccca tcaacaacaa ccggtacgcc
     4561 gccttccagc gacgtcagta acggcgcagt cacgaacgcg gtacgtccgt tgttcaccat
     4621 ctcttcatag cccttagttt cacgacgccc cagcgcggcg gtacgcgctt tctcctggga
     4681 gatgtaagcc gcaatcggcg cgcaatcgtc catgcgactt aacgccagca gatgaccgcc
     4741 gtcatcggca acagcaatgg aaaccgacca gttatttttc tgcgcctctt cctgacctgc
     4801 ggcaataatt gcactcgcca tttgctggct aagaatgact ttagttttca tttttttatt
     4861 ccttttcaag ggcttgttct acaatttcaa tccagtgacg cactgaggta cgaccggcgc
     4921 tcgccagatg cgtctggcaa ccaatgttgg cggtgacgat catttccggt ttgccgcttt
     4981 ccagcgcatt cattttgtta tcccgcagct ggcgtgccag atcgggatgc gttaacgcat
     5041 atgttcccgc tgaaccgcag cacagatggc tgtcgggaac gtccgttaag gtaaatccaa
     5101 gacgaagcaa cactttttcc acttcgccgt tcagcttttg cgcatgttgt agggtacacg
     5161 ggcagtggaa ggccagtttt ttatcgccgc gaactgccag tttttccagc ggttcctcgc
     5221 gcagaagctc gactaaatcg accgccagtt cactgacttg acgcgcttta tcggcatata
     5281 acgcatcgtt tttcagcatc tgcccgtact ctttgacaaa cgcgccgcaa ccgctggcgg
     5341 tttgcaaaat tgcctcggca cctgcttcaa tcgcgggcca ccaggcatca atattattgc
     5401 gcgcccgtgc cagccctttc tcctgcgcat taagatgata gtccaccgcg ccacaacagc
     5461 ctgcttcgtt agctgacatg acgctgatcc ccagacgatc cagcactcgc gcagttgccg
     5521 cgttggtgtt gggcgaaagc gtaggctggg cgcagccttc cagcattaaa acccgacgct
     5581 tatggcgcag cggcggacgc ggtttagctt tcaccgtttc agcaggcagt tttgctctga
     5641 cctgttccgg taaaaacggt cgcagcacca gccctacctg cgtcagcgca cggaagaccg
     5701 ccggacgcgg cactacctgg cgcaatcctt cgcgcagcat tcgctccggc agtgggcgtt
     5761 tcactttctg ctcgacaata tcacgcccga tatccagcaa attgtgatag cgcacaccag
     5821 aaggacaggt ggtttcacaa ttgcggcaag tgaggcagcg atcgagatgc tcctgtgttt
     5881 taagcgtgac ttcgttgcct tccagcacct gtttaatcag atagatgcgc ccgcgcggcc
     5941 cgtccagttc atcgcccaga agctgatagg ttgggcaggt tgcggtacaa aatccgcagt
     6001 gaacacaggc gcgcaggatg ctgtcggctt ccagcgcgcg cgcgttctgc cgcatctctt
     6061 cagttaattg ggtttgcata gcctgctcct caaagttccg cgtacatgcg accggggtta
     6121 aacacgccgc aagggtcgag ctgctgttta agctgctggt gatagcggaa taaaggagcc
     6181 gatagcgggg caaagccacc atctccggca ctaaagcggg tcgcatgacc gccagcgttg
     6241 cgggcgatgc gatggatttg attgtcctcg gctgtcgatt tcagccagcg taacgctccg
     6301 ccccagtcga tcagttgctc gccgggtaaa tccatcatcg gcgcatcgct gggtaatgaa
     6361 atgcgccata aggtacctgg taacgagaag aacggcagtt gttgttcacg caattgctgc
     6421 cagaactgac ccgcaacctc ttcgcccccc agcagttcac gcgccgcttt taccgatcct
     6481 tcgccgccct caaggcggat ccacaacgca ttgtcgaagt aacataagcc actaatgggt
     6541 agcggctgga gttgccactg ggcgatttcg ttaatggctt cttgcaggct gatttcccga
     6601 cgcaggctca gggaggcgcg cggtcgcggt aacactttca tcgagatttc agtgagcacg
     6661 ccaagacaac cgtagcttcc ggccattaac cgtgagagat cgtatccggc aacgtttttc
     6721 atcacttcgc caccaaaacg cagatgtttt ccagcgccgg taatgatgcg cgtgccgagg
     6781 acaaaatcgc ggaccgaacc gctccacggg cgacgcggcc ccgccagccc gcaggcgacc
     6841 atcccgcccc aggtggcttc ttcaccataa tgcggcggct cgcaggggag catttgcccg
     6901 gcgctctcca gcgccgcttc aatcgccacc agcggcgttc cggcacgcgc ggttatcacc
     6961 agctcggtcg ggtcgtaatt aacaatgccg cgatgacaac gcacatccag cgtttgcccg
     7021 gtgacagggc gacctaaaaa ggctttgcta ttgctgccct gaatcaccag cggcgtttta
     7081 tcgctaattg cctgattcac ctgctccagc agcgcctggc tgtaatcaca ctcgcgtagc
     7141 atcagaaacg ctccagttca gggaaaggta aatgaccgtg atgcacatgc atggcaccaa
     7201 attcagcaca gcggtgtagc gtgggaatgt ttttcccagg gttcagcaaa ccatcggggt
     7261 caaacgccgc cttgaccgca tggaaggtcg tgatttcatc gctgttgaac tgggcgcaca
     7321 tttgattaat tttttctcgc ccgatgccat gttccccact gatgctgccg ccaacttcaa
     7381 cgcagagttc gaggatcttc ccacccagct cttccgcgcg ggcaaattca ccgggttcgt
     7441 tggcatcgaa aaggattaac gggtgcatgt tgccatctcc ggcatgaaag acgttggcaa
     7501 cacgtaaatc atattgctgc gataaacggg caatgccttc cagtacgcca ggcagggcgc
     7561 gacgcgggat ggtgccatcc atgcagtagt aatccgggga gatacgtcct accgccggga
     7621 acgcattttt gcgaccggcc cagaaacgta cgcgctctgc ttcgtcctgt gccagacgga
     7681 cgtcagtcgc gcccgctttc aacaagatgt cgttaacccg ctcgcagtct tcctgtacgt
     7741 cagactccac gccgtccagc tcgcataaca aaatcgcttc ggcgtcgacg ggataaccgg
     7801 catgaataaa atcttccgcc gcgcggatcg acaggttatc catcatctcc agcccgccgg
     7861 ggataatgcc attggcgatg atgtcaccaa ctgcaagtcc ggctttttct accgagtcaa
     7921 agctggctaa cagaacccgc gccacgggcg gcttcggcag cagttttacc gtcacttcgg
     7981 tggtcacgcc gagcatacct tccgatccgg tgaacagcgc cagcaggtca aaaccaggtg
     8041 aatccagcgc gtccgatccc agcgtcagcg cctcgccgtc cagcgtttgc acttcaattt
     8101 tcagcaggtt atgtacggtc agaccatatt tcaggcagtg gacgccgccg gcattttctg
     8161 ccacgttgcc gccaatggaa caggcgattt gtgaggaagg gtccggtgcg tagtagagat
     8221 tatgcggtgc aacggcctgg gagatcgcca ggttacgcac gcctggctgc acgcgcgcgc
     8281 ggcgaccaac ggggttaatg tcgaggatct ctttaaagcg cgccatcacc aacaacacac
     8341 ctttttccag cggcagcgcg ccaccagaaa gcccggtgcc tgcaccacgg gtcaccaccg
     8401 gtacacgcag gcgatggcag acagcaagaa tcgctgtcac ctgttccatt tgcttaggca
     8461 gaacaaccag taatggacgc gtgcgatacg cgctcaaccc gtcacactcg taaggaatga
     8521 tctcctcatc ggtatgcagg atctcaagtc cagggacatg ctcacgcagt gccatcagta
     8581 ccgatgtgcg gtcgacatcg ggtaaagcgc catcaagacg ctcttcgtac aagatgctca
     8641 tgagtaggct tcgctttgtt gtgttgtgtg gcagctgatt tttgcgcgct gcttctgtga
     8701 acagttatta agcgggcttt tcgttttcgt ctatctcttt agctaccggt cagaccattt
     8761 tttttccagc tctgtgacct tgccttggtt aactcaatgt taaattgatg taacataatc
     8821 acttacgtga tgtgcgtgtt ttgcgagtta agaacagaaa aattggtcct acctgtgcac
     8881 gaggtccggg aatgaaagat gaacgtcgcc ctatttgcga agtggttgca gagagtatcg
     8941 aacggttaat tatcgacggc gtactgaagg tcggtcagcc acttccctcg gaacgtcgac
     9001 tgtgtgaaaa gctcggcttc tcacgctccg cactgcgtga agggctgacc gtgctgcgcg
     9061 ggcgcgggat tattgaaacg gcgcagggtc gcgattctcg tgtcgcacgg cttaatcggg
     9121 tgcaggacac cagcccgctg atccatctgt tcagtacgca gccgcgaacg ctgtacgatc
     9181 tgctcgacgt tcgcgcatta ctggagggcg aatcggcaag gctggcggca acgctgggaa
     9241 cgcaggctga ttttgttgtg ataacccgct gttatgaaaa aatgctcgcc gccagtgaga
     9301 acaacaaaga gatttcgctg atcgaacatg cgcagttgga tcacgctttc catctcgcca
     9361 tttgtcaggc ttctcacaat caggtgctgg tgtttacgct gcaatcattg accgatctga
     9421 tgtttaattc agtgtttgcc agcgtaaata atctctacca tcgaccacag caaaaaaagc
     9481 agatcgatcg ccagcatgcg aggatctaca acgcggtgtt gcagcggctg ccgcacgtcg
     9541 cccagcgcgc agcacgcgat catgtgcgga ccgtgaaaaa gaatctccac gatatcgagc
     9601 tggaaggcca ccatttgatt cgctcggcgg tgccgctgga gatgaacctg agttagctgg
     9661 tattaaatct gcttttcata caatcggtaa cgcttgtacg gctccgcccc aatgcgttcc
     9721 agcatgttat tcatgcctgt attggtttcg aggatccatg acatctccag cgcatcgatc
     9781 ttccggcggg caaacggatc gcgtaaggct tcaatcaata acagcgcaat caccgggccg
     9841 atgcggctga actgatactc gtcgcgcacg cccatcagcg gtactcgcgc agttcgcaca
     9901 ccgctgactt tcaaacgcca cagcaatttt gcccagccga agggaaagag cgatccgttc
     9961 agatcggcaa tcgcctcgtt gatgttcggc aagccgacaa taaacgcgca gggtgcagaa
    10021 tcaatctcag cgatatagat catatcgtcc ggcaccagat atttaagttg atcgcccatg
    10081 gtcgcgaatt catgttcggt aaacggcaca aatccccagt tgtgctgcca gccagagttg
    10141 aaaatctcac gcaggatctg catttcttcg gcaaaccgct gacgattgat gcagcgaatg
    10201 gtcacctttt tgcgcacctg atccatcagt ttttttagcg ccggagagaa agtgagatcg
    10261 gttcgctgca tccaccacgc cagtaaatca atgcctttgt gataacccag ttgttcaata
    10321 tgcgcggcat accacggttt gccgtgtggc atcatcgcac agggtggtgt gtcaaaacct
    10381 tcaatcagta atccgctttc ctgattgata ttcaggctga aaggaccgct gatcttactt
    10441 gcaccttgtg acttcaacca cgcttccgct gcgccaaaca acgcggcaaa aacctgcgga
    10501 tcatcaatgg cgtcaatcat gccgaaatga ccggtatctt caccgtaacg ctcacggtgc
    10561 aaggtatcta tttgcgcggt aatacgccca actatctgcc ccgctttttt tgccacccac
    10621 gcctgccaga tgatatggtc cgtccccgga tttttcgcag acaaatgctc gttgcgttct
    10681 atgaataaag ggggtatcca gtttg
//
LOCUS       DAZGTR010000144        10453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGTR010000144 DAZGTR010000000
VERSION     DAZGTR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.146"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(91..166)
                     /locus_tag="ACHLCY_004141"
     tRNA            complement(91..166)
                     /locus_tag="ACHLCY_004141"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Lys,seq:ttt)
     gene            complement(200..275)
                     /locus_tag="ACHLCY_004142"
     tRNA            complement(200..275)
                     /locus_tag="ACHLCY_004142"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(240..242),aa:Lys,seq:ttt)
     gene            complement(422..497)
                     /locus_tag="ACHLCY_004143"
     tRNA            complement(422..497)
                     /locus_tag="ACHLCY_004143"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(462..464),aa:Lys,seq:ttt)
     gene            complement(501..576)
                     /locus_tag="ACHLCY_004144"
     tRNA            complement(501..576)
                     /locus_tag="ACHLCY_004144"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(541..543),aa:Val,seq:tac)
     gene            complement(726..801)
                     /locus_tag="ACHLCY_004145"
     tRNA            complement(726..801)
                     /locus_tag="ACHLCY_004145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(766..768),aa:Lys,seq:ttt)
     gene            complement(804..879)
                     /locus_tag="ACHLCY_004146"
     tRNA            complement(804..879)
                     /locus_tag="ACHLCY_004146"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(844..846),aa:Val,seq:tac)
     gene            complement(1015..1090)
                     /locus_tag="ACHLCY_004147"
     tRNA            complement(1015..1090)
                     /locus_tag="ACHLCY_004147"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1055..1057),aa:Lys,seq:ttt)
     gene            complement(1255..2046)
                     /gene="cpoB"
                     /locus_tag="ACHLCY_004148"
     CDS             complement(1255..2046)
                     /gene="cpoB"
                     /locus_tag="ACHLCY_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP0371290.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(2056..2577)
                     /gene="pal"
                     /locus_tag="ACHLCY_004149"
     CDS             complement(2056..2577)
                     /gene="pal"
                     /locus_tag="ACHLCY_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP0371291.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2612..3904)
                     /gene="tolB"
                     /locus_tag="ACHLCY_004150"
     CDS             complement(2612..3904)
                     /gene="tolB"
                     /locus_tag="ACHLCY_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP0371292.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(4037..5257)
                     /gene="tolA"
                     /locus_tag="ACHLCY_004151"
     CDS             complement(4037..5257)
                     /gene="tolA"
                     /locus_tag="ACHLCY_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0371293.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAA
                     AEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIE
                     SKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQA
                     VYEVFKNAPLDFKP"
     gene            complement(5322..5750)
                     /gene="tolR"
                     /locus_tag="ACHLCY_004152"
     CDS             complement(5322..5750)
                     /gene="tolR"
                     /locus_tag="ACHLCY_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP0371294.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5754..6446)
                     /gene="tolQ"
                     /locus_tag="ACHLCY_004153"
     CDS             complement(5754..6446)
                     /gene="tolQ"
                     /locus_tag="ACHLCY_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP0371295.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(6443..6847)
                     /gene="ybgC"
                     /locus_tag="ACHLCY_004154"
     CDS             complement(6443..6847)
                     /gene="ybgC"
                     /locus_tag="ACHLCY_004154"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0371296.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6997..7290)
                     /gene="ybgE"
                     /locus_tag="ACHLCY_004155"
     CDS             complement(6997..7290)
                     /gene="ybgE"
                     /locus_tag="ACHLCY_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP0371297.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(7290..7403)
                     /gene="cydX"
                     /locus_tag="ACHLCY_004156"
     CDS             complement(7290..7403)
                     /gene="cydX"
                     /locus_tag="ACHLCY_004156"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP0371298.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(7418..8557)
                     /gene="cydB"
                     /locus_tag="ACHLCY_004157"
     CDS             complement(7418..8557)
                     /gene="cydB"
                     /locus_tag="ACHLCY_004157"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0371299.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8573..10141)
                     /gene="cydA"
                     /locus_tag="ACHLCY_004158"
     CDS             complement(8573..10141)
                     /gene="cydA"
                     /locus_tag="ACHLCY_004158"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP0371300.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2564 a         2832 c         2501 g         2556 t
ORIGIN      
        1 gtttcgctgc gctcaactca aacctgctgc aggttcgaat ctttaagtat cgcagatagc
       61 aaaaaagcgc ctttagggcg ctttttacat tggtgggtcg tgcaggattc gaacctgcga
      121 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgaccccttg cgggattatg
      181 ctgctttaat cattcaggat ggtgggtcgt gcaggattcg aacctgcgac caattgatta
      241 aaagtcaact gctctaccaa ctgagctaac gacccatacg ggtgttacct gaaagatttc
      301 tactcggggc caatctttaa gattggtgtg tcgtgcagga tgactcgctt cgcttgccct
      361 acgggccgtt gctggcgcaa cgttatcctt cacgcttaac atctgagttt gatgttaaaa
      421 ttggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc
      481 aactgagcta acgacccgag tggtgggtga tgacgggatc gaaccgccga ccccctcctt
      541 gtaagggagg tgctctccca gctgagctaa tcacccgata ctgcgctgga tactacaaac
      601 tacttccacc gaacccaccg taattagtgg tgggtcgtgc aggatgactc gcttcgctcg
      661 ccctacgggc cgttgctgac gcaacgttat cctacacgct taacatctgt cttcgatgct
      721 aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
      781 taccaactga gctaacgacc cggtggtggg tgatgacggg atcgaaccgc cgaccccctc
      841 cttgtaaggg aggtgctctc ccagctgagc taatcaccca atactacgct ggatactaca
      901 actaactatg ttagtggtgg gtcgtgcagg atgactcgac ttcgtctcgc ccttcgggcc
      961 gttgctgatg caacgttatc cttcacgttt tactaccgct tccaccttag cgattggtgg
     1021 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
     1081 ctaacgaccc actttttcgt tgctttcggt ttgtttgata tcccgtggca acggcggcat
     1141 atattactga tttcagactt gagcgcaaca aaaatttcga tgcagatcac tcaactgctt
     1201 atgattcgca cgacacgacc agaaataatg cgacttctgg tcgtgtgtta tgcattacat
     1261 cgcgttcaga cgtttttgtg cctgtttagc gccatcagta ccagggtatt tactgataac
     1321 ctgctggtat acggctttcg cttttgcggt gtcacctttg tcctgcatga tgacgccgac
     1381 tttaaacatc gcatcagcag cctttggtga cttcggatag tttttcacta ccgaagcaaa
     1441 atagtacgcc gcatcatctt ttttaccctt gttgtagttt aactgaccca gccaataatt
     1501 ggcgtttggc aggtaagttg aatcagggta atttttgatg aaattctgaa atgccaccat
     1561 tgcgtcatcc tggcgggatt tatcctgcac cagcgcaata gctgcattgt aatccgtgtt
     1621 tgcatcgccg cttttcaccg gcgcgccagc attcgcagta ccagcatcag ctgtcggcgt
     1681 cgttgatgcc gccgcaccgc tttgatcgct gctggttgat tgcgccgctg caccaccgct
     1741 gctgaggctg tcgatctgca acaggatctg cttttgccgc tccacgacct gattcagttg
     1801 atactgattt tcctgaatct gaccacgcag ggaatcaata tcggattgat tatcagaaag
     1861 ttgttgctgg agttgggtta aaagctggct gtgagcatta gaaatacgct caagttgagt
     1921 gacgcggtct tcgaccgagc ctgagccgac actactgatt ggtgcctgag caaaagcggc
     1981 ccagggggcc gctataccaa ccagtaacga cagactcaat agttgatgtc tgaagttact
     2041 gctcatgcaa ttctcttagt aaaccagtac cgcacgacgg tttttggagt atgccgcttc
     2101 gtcatgaccc agtactgcag gtttttcttt accgtaagaa acgatggaga tctggtctgc
     2161 agaaacgcct ttaccctgca ggtacatctt aacggcgttc gcacgacgtt cacccaggga
     2221 gatgttgtat tccggagtac cacgttcgtc cgcgtgacct tctacggtga ctttgtaaga
     2281 cgggttgcta cgcaggaagt ttgcatgtgc atccagcatt tgagcgaagt cagaacggat
     2341 atcgtacttg tccagatcga agtaaacgat gttgttctgc tgcagctgtt gcatttgcag
     2401 acgagcctgc tcttcggaag acatgttgcc gttgccgccg ttcgcatcca taccagtgcc
     2461 ggcacccagc atgccttcgc tgccgtcatt gctggcgttc ttgttggaag aacatgccgc
     2521 aattgccata acaggcagag caatcatcag ccctttcagc actttgttca gttgcatttc
     2581 aatgattcct ttactattca attaattatt atcacagata cggcgaccag gcagggaatt
     2641 tgacctgtcc atcagttgcc ggaagacgcg ctttgaaacg cccatctgta gaaaccaaat
     2701 tcagcacgga tcccatcccc tgagaagagc tgtagattac catagtgccg ttaggtgcca
     2761 gacttggcgt ttcatccagg aacgtggacg acagaacttg tacgcctccc gttgccagat
     2821 cttgtttggc aatgtgctgc tgcccaccat tggagctgac cattaccata aatttaccgt
     2881 cgctgctgac atccgcatcc tggttctgcg aaccttccca ggtaatacgt tgtggcgcac
     2941 cgccgttgat attcacttta taaacctgcg gacgaccggc ctggtcagaa gtaaatgcca
     3001 ggttctggct atccgggaac caggtcggtt cggtattgtt actgcgacca tcagtcacct
     3061 ggcggatctg accagaagcc aaatccatta cgtacaggtt cagactaccg gttttcgaca
     3121 aggcgaatgc cagtttgctg ccgtctggcg agaatgcagg tgcaccgttg tgacgcggga
     3181 atgaagccac ctgacgtaca gcgccatttg ccagcgtctg aataaccagc gcggaacgac
     3241 cgctttcgaa ggtcacataa gccagtttag aaccgtctgg tgaccacgcc ggagacatca
     3301 gcggctgcgg tgaacggtga acgacaaact ggttgtaacc gtcatagtca gatacgcgca
     3361 gttcatacgg gaactgaccg ccgttggtct gaacaacgta ggcaatacga gtacggaacg
     3421 cacctttaat gccggtcagt ttttcaaaca cttcatcact ggcggtatga ccagcataac
     3481 gcagccactg cttgttcact ttgtacgagt tctgagcaag tacagtaccc ggtgcgccgc
     3541 cagtgtcaac aagttgataa gcaacattgt aagaaccatc cggattcgga gtgacctgac
     3601 cgacaactac agcgtcaatg cccagtgcgg accatgcagc tggttgtact tcctgcgcac
     3661 tacccggctg ctgtggcaga cgagcgcgat ctaacggatt aaatttaccg ctgttacgca
     3721 agtcagcagc aacgatgccg ccaatatctt caggtgccgc accaggcccc gcccactgga
     3781 aaggaacaac accaatagga cgaccggaat ctacaccgct gtcgatcaca atgcggactt
     3841 cagcatgcag aactgatgcc cacagtatga gaaaaccaaa tgctactcgt aatgcctgct
     3901 tcatcatatc tcccttatct ggaccgatgg cccacgataa tttagcagaa tgttaacaaa
     3961 ctaaaataca caaaactacc agaaccccgt ggcaaccggt gcctgatgtt gaccgtccga
     4021 acagtcaaca tcgcgattac ggtttgaagt ccaatggcgc gtttttgaac acttcatata
     4081 ctgcctggct tggtggtttc gggatcttcg caagtttagc tgctgccaac gcagcctgac
     4141 aaagtgcggg atcgccacct tcaggtttga tatccagtaa cataccatcg ggtgccagtt
     4201 ttatgcgcag cgtacaggtt ttgcctgcat aggacgatgc gtcatagaac ttactttcga
     4261 tagcagattt aatctgcccg gcatagttat tgatatcggc ccctgatgcg ccattgtttt
     4321 tagtattacc actcccggca ggcgaagcat tgttcccttt cgcccctccc cccgttttcg
     4381 gtgcattctt accagagctt agctcaccga aaatatcatc tgcctctgcg gccgcttttg
     4441 ctgcagcggc tttttcagct gcagcttttg ccgctgccgc ttttttgtct gcggcggctt
     4501 tttctgccgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc ttatcagctg
     4561 cagccttttc agcagccgct ttcttctcag cttctgcttt ggctttttca gcagcttcag
     4621 ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct ttcttcttca
     4681 gtgccgcagc ggctgcctcg gcttttttct gcgcttcggc tgcggctttg gcggcttctg
     4741 cttctgcttt tttctttgcg tctgcagccg ctttcttcgc tgcttcttcc gcagctttag
     4801 catctgcttc ggccttcgct ttagcatctg ccgccgcttt cgctgccgcc tcttcagctt
     4861 gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc tgctcctgag
     4921 ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct tgtttctcac
     4981 gcagttcttc agcagcctgc tgttccttca tcttgcgctg ttcatcagaa cgcttcgcgc
     5041 ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa tcaaccatga
     5101 cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc tcatcgaacg
     5161 aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata attatcgccc
     5221 gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa aaactgttcg
     5281 cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg ctgcgtcatt
     5341 aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat tatttcatcg
     5401 taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc cttgaaacgg
     5461 ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc
     5521 accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt atcgttactg
     5581 ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg ggtgatgatg
     5641 ggcgctgtcg ccatgaagat cagcaacagc accagcagta cgtccagcaa cggtacaatg
     5701 ttgatttcgg acttaagttc gcgacgacct cgtccacgcg ctctggccat gacttacccc
     5761 ttattgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata
     5821 aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgaggcggtt gtaggccata
     5881 actgccggga tagcggcaaa cagaccaatt gcagtcgcaa tcaacgcttc tgcgataccg
     5941 ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa ggcgtgcatg
     6001 atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac cgtaccgagg
     6061 aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg catagcacgc
     6121 gacgcccctt ccactacggc ttccggcgca tggctattgg cacgatgcag gcgcacaaac
     6181 tctttgaacc cgctgtaaaa tatttgttcc gaaccagtca gattatcccg tttcccctga
     6241 ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc aaacgcttcg
     6301 gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga taataatggc ccaggatgcg
     6361 attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc cttcaggaac
     6421 aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac ttgggaagcg
     6481 cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct tcattcagca
     6541 aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg
     6601 ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg
     6661 tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat
     6721 gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca
     6781 ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga aacagcgttg
     6841 tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc tgctactata
     6901 cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc
     6961 tggagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga aaatcagccc
     7021 gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg
     7081 cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca gcaataaacc
     7141 atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc aaaacataca
     7201 tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct tgtccattac
     7261 cgcatacaaa gtcgcgataa tcttactcat cagatgtctt cttgaccggc tttgcctgat
     7321 tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag aagcgttccc
     7381 agaatccatg cgaaatacca catttttagc tccttactta gtacagagag tgggtgttac
     7441 gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag gcggtgtaga
     7501 gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc gtcagctggc
     7561 tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac ggcatcacaa
     7621 acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca
     7681 caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc ggcagaacca
     7741 cacccagtgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc agccatgcgc
     7801 cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg
     7861 atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt gcgaaacaga
     7921 ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc agttcgccca
     7981 cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg ctcaccacgc
     8041 ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag tacagacgca
     8101 gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct acaccaatta
     8161 ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta cgccagcggg
     8221 tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa gacgccagca
     8281 cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc ggccaggcag
     8341 caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa
     8401 tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc
     8461 ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg ccaaccagca
     8521 gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg tcttagcgtg
     8581 ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc aggctgcttg
     8641 ggccgaggcg tgcaaatttg aacattaaga acaattctgc caccaggaac agggtataca
     8701 ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac gagttcgcca
     8761 cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca gccacgaacc
     8821 agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg cgcagaagcc
     8881 attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg atgattgcca
     8941 gaagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc gctacacgcg
     9001 ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg
     9061 tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg aactggtcac
     9121 gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac gccttcatcc
     9181 cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata accggggtat
     9241 ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca aatttgttcg
     9301 tctcctcttc ctgatcagga atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt
     9361 cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg
     9421 attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg gcagcgatag
     9481 caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat gcgctgatac
     9541 cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg aatttcacct
     9601 gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc atagtttcaa
     9661 agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaaccaga atccacagtg
     9721 cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga actttaccca
     9781 gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag aaggccatca
     9841 gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa tagtaagacc
     9901 agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga gcgaagttga
     9961 taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg ccggagagga
    10021 cgtagaccgt ttccataatg gccagcagga acgccatacc gagcgtcagt ggcacaaaaa
    10081 ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact atatctaaca
    10141 tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag aaagggtcac
    10201 acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga atgcacacaa
    10261 aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat cccacgtata
    10321 tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt tgcatttttc
    10381 gacggtgatc aatttatagc taaattaccg cctttcagcc aatttgatcg agaacaattt
    10441 atctcttttt gat
//
LOCUS       DAZGTR010000145        10438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGTR010000145 DAZGTR010000000
VERSION     DAZGTR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.147"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..623
                     /gene="ssuE"
                     /locus_tag="ACHLCY_004159"
     CDS             48..623
                     /gene="ssuE"
                     /locus_tag="ACHLCY_004159"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP0371301.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            616..1575
                     /gene="ssuA"
                     /locus_tag="ACHLCY_004160"
     CDS             616..1575
                     /gene="ssuA"
                     /locus_tag="ACHLCY_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP0371302.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGAVRVLKDGTDLNQTGSFYLGARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1572..2717
                     /gene="ssuD"
                     /locus_tag="ACHLCY_004161"
     CDS             1572..2717
                     /gene="ssuD"
                     /locus_tag="ACHLCY_004161"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP0371303.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2728..3519
                     /gene="ssuC"
                     /locus_tag="ACHLCY_004162"
     CDS             2728..3519
                     /gene="ssuC"
                     /locus_tag="ACHLCY_004162"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP0371304.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3516..4283
                     /gene="ssuB"
                     /locus_tag="ACHLCY_004163"
     CDS             3516..4283
                     /gene="ssuB"
                     /locus_tag="ACHLCY_004163"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0371305.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4490..7102)
                     /gene="pepN"
                     /locus_tag="ACHLCY_004164"
     CDS             complement(4490..7102)
                     /gene="pepN"
                     /locus_tag="ACHLCY_004164"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP0371306.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7368..8570
                     /gene="pncB"
                     /locus_tag="ACHLCY_004165"
     CDS             7368..8570
                     /gene="pncB"
                     /locus_tag="ACHLCY_004165"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP0371307.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8739..10139
                     /gene="asnS"
                     /locus_tag="ACHLCY_004166"
     CDS             8739..10139
                     /gene="asnS"
                     /locus_tag="ACHLCY_004166"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP0371308.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2358 a         2690 c         2807 g         2583 t
ORIGIN      
        1 gttatcaatg ttaacaaaaa aagaacaatt ggttataagg agagagtatg cgtgtcatca
       61 ccctggcggg tagtcctcgc tttccttctc gctccagctc cttgctggaa tatgcgcggg
      121 aaaaactaaa ttgcctggat gtagaggttt atcactggaa tctgcaaaac ttcgccccgg
      181 aagatctgct ttacgctcgt ttcgatagtc cggcactcaa gaccttcacc gaacagctgc
      241 aacaggctga tggactgata gtcgctacac ctgtgtataa agccgcctat tccggcgcgc
      301 tgaaaaccct gctcgacctg ctgccagagc gcgctttgca aggcaaagtg gtgctaccgc
      361 tggcgacggg cggtacggtg gctcatctgc tggcggtcga ttatgccctt aagccagttt
      421 taagtgcgat gaaagcccag gagatcctgc acggcgtgtt tgccgatgac tcacaagtta
      481 ttgattacca tcacaaaccc cagttcacgc caaatctgca aacccgtctt gataccgcgc
      541 tagaaacttt ctggcaggca ttgcaccgcc gcgatgttca ggttcctgac cttctgtctc
      601 tgcgaggtaa tgtccatgcg taacatcatt aaactggcgc tggcgggatt gcttagcgtc
      661 tctacttttg cggttgctgc agaatcctcg cctgaagcgt tacgtatagg ctatcagaaa
      721 ggcagtattg gtatggtatt ggcaaaaagc caccagttac tggaaaaacg ctatccgcaa
      781 tcaaaaatct cctgggtgga gttccccgcg ggtccgcaaa tgttggaagc gttaaacgtt
      841 ggcagtattg atctcggcag taccggggat attccgccaa tctttgccca ggctgccggg
      901 gctgatttgg tatatgtggg cgtcgagcca ccgaagccca aagccgaagt gattctggtg
      961 gcagaaaaca gcccgatcaa aaccgtagcc gatcttaaag gtcacaaagt tgcctttcag
     1021 aaaggttcca gttcacacaa ccttttactg cgtgcactgc gtcaggccgg acttaagttt
     1081 accgatatcc aacccactta cctgacgccc gctgatgccc gcgccgcgtt ccagcaaggt
     1141 aacgttgacg cctgggctat ctgggatccc tactactctg ctgcattatt acagggcgcc
     1201 gtgcgggtgc tgaaagacgg caccgatctc aatcaaactg gatcgtttta tctgggagct
     1261 cgtccctatg cagaaaaaaa cggcgctttt attcagggcg tactcgcaac ctttagtgag
     1321 gccgatgcgt taacccgcag ccagcgcgag caaagcatcg ctttactggc aaaaacgatg
     1381 ggcttaccgg caccggtgat tgcctcttac ttagatcatc gccctcctac caccatcaaa
     1441 ccggttaacg ccgaggttgc cgccttacag cagcaaacgg cagatctgtt ttatgaaaat
     1501 cgtctggtgc cgaaaaaagt cgatattcgc cagcgcatct ggcagcccac tcaactggaa
     1561 ggaaaacaat tatgagtctg aatatgttct ggtttttacc gacccatggt gacgggcatt
     1621 atcttggaac ggaagaaggt tcacgcccgg ttgatcacgg ctatctgcaa caaattgcgc
     1681 aagcggcgga tcgtctgggc tataccggtg tgctgatccc aacggggcga tcgtgtgaag
     1741 atgcgtggct ggtggcggcg tcgatgatcc cggtgacgca gcggctgaag tttcttgtcg
     1801 ccctgcgccc cagcgtaacc tcacctaccg ttgccgctcg ccaggccgcc acgcttgacc
     1861 gtctctcaaa tggtcgtgcg ttgtttaatc tggtcacagg cagcgatcca caagagctgg
     1921 caggcgacgg cgtgttcctt gatcatagcg agcgctacga agcctcggcg gaatttaccc
     1981 aggtctggcg gcgcttattg ctgggggaaa ccgtcaattt caacggtaaa catattcatg
     2041 tgcgcggagc aaaactgctc ttcccgccga ttcaacagcc ttatcctccg ctttactttg
     2101 gcggatcgtc agacgttgcc caggagctgg cggcagaaca agttgatctc tacctcacct
     2161 ggggcgaacc gccagaacta gtaaaagaga agatcgaaca agtgcgggcg aaagctgccg
     2221 cgcatggacg caaaattcgt ttcggtattc gtctgcatgt gattgttcgt gaaactaacg
     2281 acgaagcgtg gcaggccgcc gagcggttaa tctcgcatct tgatgatgaa actatcgcca
     2341 aagcacaggc cgcattcgcc cggacggatt ccgtaggaca acagcgaatg gcggcgttac
     2401 ataacggcaa gcgcgacaat ctggagatca gccccaattt atgggcgggc gttggcttag
     2461 tgcgcggcgg tgccggaacg gcgctggtgg gcgatggtcc tacggtcgct gcgcgaatca
     2521 acgaatacgc cgcgcttgga atcgacagct ttgtgctttc gggctatccg catctggaag
     2581 aagcgtatcg ggtcggcgag ttgctgttcc cacatctgga tgtcgccatt ccggaaattc
     2641 cccagccgca gccgctgaat ccgcaaggcg aagcggtggc gaatgatttt atcccccgta
     2701 aagtcgcgca aagctaagga agaaataatg gcaacgccag tgaagaagtg gttattgcgc
     2761 gttgccccct ggtttttacc ggtgggcatc gtggcggtgt ggcaactggc ctcctcagtt
     2821 ggctggctgt cgacgcgtat tttgccctca ccggaagggg tggtgacggc gttctggacg
     2881 ctctccgcca gcggcgaact gtggcagcat ctggcgatca gttcctggcg ggcgctgatt
     2941 ggcttttcaa ttggcggatc gctggggttg attttgggac tgattagcgg gctatctcgc
     3001 tggggagaac ggctgctgga tacctcaatt cagatgttgc gcaacgtacc gcatctggcg
     3061 ctgatcccgc tggtgatttt gtggtttggc attgatgaat ccgcaaaaat ctttctggtg
     3121 gcgctcggca cgctattccc catttatatc aacacctggc atggcatccg taatatcgat
     3181 cgcggtctgg tggaaatggc gcgtagctat ggattatccg gcataccgct gtttatccat
     3241 gtgatcctgc ctggtgccct gccctcaatt atggtcggcg tgcgttttgc gttgggcctg
     3301 atgtggctga cgctgattgt tgccgaaacc atttctgcca attcaggcat tggttatctg
     3361 gcgatgaatg cgcgggagtt tttgcaaacg gacgtggtgg tagtcgctat tattctttac
     3421 gccctgctcg gcaaacttgc tgacgtcagc gcgcagctgt tagagcgcct ctggttgcgc
     3481 tggaacccgg cttatcattt gaaggaggcc acggtatgaa tactgctcgt ctgaaccagg
     3541 gcacaccatt gttgctcaat gcagtaagca aacattacgc ggaaaatatc gtcctgaacc
     3601 aactggattt acatattccg gcaggtcagt ttgtggcggt ggtgggccgc agcggtggtg
     3661 gcaaaagtac cctgctgcgc ctgctggcag gtctggaaac gccaaccgca ggcgatgtgt
     3721 tagcgggcac cacaccgctg gctgagattc aggaagacac gcgaatgatg tttcaggatg
     3781 cgcgtctgct gccgtggaaa tcggtgattg ataacgttgg gttaggcctt aaaggccagt
     3841 ggcgcgatgc cgcgcgtcgg gcgttggctg cagtaggact ggagaatcgc gccggggaat
     3901 ggcctgccgc actttctggc gggcagaaac agcgtgtggc gctggcaaga gcgttgattc
     3961 atcgaccggg tttattgttg cttgatgaac cgctcggggc gctggacgcc ttaacgcgac
     4021 tcgagatgca ggatttgatt gtgtcacttt ggcagcagca cggctttacc gtactgttgg
     4081 tgacgcatga tgtgagcgaa gcggtagcaa tggctgaccg ggtgttgtta attgaagagg
     4141 gaaaaattgg tctggatttg acagtggata tcccccgtcc gcgtcgatta gggtcggtgc
     4201 gattagcgga gctggaagcg gaagtattgc aacgggtaat gcggcgtggg cattctgaac
     4261 aactgattcg gcgtcatggt taatatgccg gatgtggcgc acaaaaccag cattttcaac
     4321 ggactagtag gcctgataag acacgtcaag catcgcatca ggcagttggg cacggctgcc
     4381 ggatgcggcg tgaacgcctt atccagccta caaaaccagc aatttcaacg gagttgtagg
     4441 cctgataaga cgcgtcaagc gtcgcatcag gcattttggc tcaaaagtat caagccagtg
     4501 ctttggtgat cttctcgtac agatcgccag agagattttc cagccctttc aactgttcca
     4561 gcgccgcgcg cattttctcc tgacgtttgg catcataacg tttcaggcga atcagcggtt
     4621 caatcagacg tgaagctacc tgcgggttac ggctgttgag gtcggtaagc atttccacca
     4681 ggaactggta accgctgcca tcttcggcat ggaacgctgc cgggttgctg cccgcaaacg
     4741 caccaatcaa cgaacgaata cggttcgggt tgctcatggt aaatgagcga tgctgcaaca
     4801 gaccacgcac cgtctccagc acattcgccg ccgggctggt ggcttgcagg ataaaccatt
     4861 tatccatcac cagaccgtcc tgatgccact tatcgtcgta ctcctgcatc agcgcgtcac
     4921 ggcaaggcag ctgtgcagca accgccgcag aaagcgctgc cagcgcatcg gtcatattgt
     4981 ttgcttcatg gtattgcttg ctcaccagca cgtcagccag atgcgtttca ccaaaagcaa
     5041 ggaagcgcag gcaggcatta cgcagagtgc gttttgcaat atcttcatgc tcaacacggt
     5101 actcgctctg gtaattcgcg ttgtaaatag ccagcagctc atccgccagt tcagtcgcca
     5161 gagtacgagt gagtgcttcg cgtacttcgg caatagcaat cgggtcgatg atatcgaaca
     5221 gttcagccat ttcattgaca gaaggcagcg tcaggatttc tgccgccagc gccggatcaa
     5281 tcttctcatc gagcagcacc gcgcggaaag cgtcagccac atgtaccggc agagacagcg
     5341 gctgcccttg ctgatgacgg gcgacgttca gcttgatgta ggttgccagc aaactttgcg
     5401 ccgcatccca acgggagaaa tcattacgcg catgacgcat caggaaggtc agttgctgat
     5461 cgctccactt atattccagt ttcactggcg cagagaattc gcacagcagc gcaggcaccg
     5521 gctggaagta gacattatca aaaacaaagg tctgttccgc ctgggtgacg ttcagcacgg
     5581 aattcaccgg atgaccgcct ttctgcaacg ggatcacttt gccttcgttg tcatacaatt
     5641 cgatggcaaa tggaatatgc agcggctgtt tttctgcctg atccggcgtg gctggcgtgc
     5701 gctggctgat ggtcagggtg tactgctcgg tttccggatt gtagtcgtct ttgacggtca
     5761 caatcggcgt gccggactgg ctgtaccaac ggcggaaatg ggagagatcg acattcgacg
     5821 catcttccat cgcctgcaca aagtcgtcgc aggttgccgc gctgccatca tggcgctcga
     5881 aatagagttg catccctttc tggaagtttt cttcgcccag cagggtgtgg atcatgcgaa
     5941 tcacttccgc gcccttctcg taaacggtca gggtgtagaa gttgttcatc tcaatgacca
     6001 tatccgggcg gatcgggtgc gccatcgggc tggcgtcttc cgcaaactgc aatccgcgca
     6061 tggtgcgcac attattgata cggtttaccg cgcgggaacc aaggtcagag ctgaattcct
     6121 gatcgcggaa gacggttaaa ccttctttca ggctgagctg gaaccagtcg cggcaggtca
     6181 ctcggttacc ggtccagtta tggaaatatt catggccgat aacgcgttca atatcgagat
     6241 aatctttgtc ggtggcggtg tcggtgcggg ccaacacata tttggagtta aagatattca
     6301 gacccttatt ctccattgcg cccatattga agaaatccac cgcgacgatc atatagatgt
     6361 cgaggtcata ctccaggcca aagcgttctt catcccattt catggagttt ttcagcgagg
     6421 tcatcgccca cggcgcgcga tcaaggttgc cgcgatcgac gtacagctcc agtgctactt
     6481 cgcgaccaga acgcgtggta aaggtgtcgc gcagtacatc aaagtcgcct gccaccagcg
     6541 caaacaggta gcacggtttc gggaacgggt cctgccactg tacccaatgg cgtccgtttt
     6601 ccagttcacc ttgcgcaacg cggttaccgt tggaaagcag gaagggatat ttggttttat
     6661 cggcaataat tttggtggta aaacgcgcca gcacgtccgg gcggtcgaga taatacgtaa
     6721 tatggcggaa accttcggct tcacactggg tgcaaagcgc atcgcctgac tgataaagcc
     6781 cttccagcgc cgtattcgcc gccgggctta tttcattaat gatcttaagc gtaaaacgct
     6841 ccggcaaatt actgatgacc agtgcgccct cttcttcttt ccaggcggtc cacggctcat
     6901 cattaatatg aacagaaacc agtttgaggt cttcgccgtt gagacgaagc ggagcatctg
     6961 atgcaccatg acggacagcc tggctgaccg cggtaacgac cgtcttttgc gcgtcgaggt
     7021 caaaggtcaa gtcaatatca gtaatctggt aatccggcgc acgataatcg tgacggtatt
     7081 tggcttgtgg ctgttgagtc ataaataacc tttagcatct tttatagagt ctggtgttca
     7141 gtctattcct gttgcgtaaa tcgcgctatg cagaatcttc atcttttcag gtacaaacgc
     7201 ctttattgct acatttttat aacatacacc gcgtaatgcc atcgaccaga aaggtggcat
     7261 atggtgtgat cggggttcaa taaattgcga aacaaggtat actccagcag ttcctgaaga
     7321 tgtttattgt actaaacgct cctgtacgag gacgctactg cgcacctatg acacaattcg
     7381 cttctcctgt tctgcactcg ttgctggata cagatgctta taagttgcat atgcagcaag
     7441 ccgtgtttca tcactattac gatgtgcatg tcgcggcgga gtttcgttgc cgaggtgacg
     7501 atctgctggg tatttatgcc gatgctattc gtgaacagat tcaggcgatg cagcacctgc
     7561 gcctgcagga tgatgaatat cagtggctct ctgccctgcc tttctttaag gccgactatc
     7621 ttaactggtt acgcgagttc cgctttaacc cggaacaagt caccgtatcc aacgataatg
     7681 gcaagctgga tattcgttta agcggcccgt ggcgtgaagt catcctctgg gaagttcctt
     7741 tgctggcggt tatcagtgaa atggtacatc gctatcgctc accgcaggcc gacgttgcgc
     7801 aagccctcga cacgctggaa agcaaattag tcgacttctc ggcgttaacc gccggccttg
     7861 atatgtcgcg cttccatctt atggattttg gcacccgtcg ccgtttttct cgcgaagtac
     7921 aagaaaccat cgttaagcgt ctgcaacagg aatcctggtt cgtgggcacc agcaactacg
     7981 atctggcgcg tcggctttcc ctcacgccga tgggaacaca ggcacacgaa tggttccagg
     8041 cacatcagca aatcagcccg gatctagcca acagccagcg agctgcactt gctgcctggc
     8101 tggaagaata tcccgaccaa cttggcattg cattaaccga ctgcatcact atggatgctt
     8161 tcctgcgtga tttcggtgtc gagttcgcca gtcgctatca gggcctgcgt catgactctg
     8221 gcgacccggt tgaatggggt gaaaaagcca ttgcacatta tgaaaagctg ggaattgatc
     8281 cacagagtaa aactctggtt ttctctgaca atctggattt acgcaaagcg gttgagctat
     8341 accgccactt ctcttcccgc gtgcaattaa gttttggtat tgggactcgc ctgacctgcg
     8401 atatccccca ggtaaaaccc ctgaatattg tgattaagtt ggttgagtgt aacggtaaac
     8461 cggtggcgaa actttctgac agccctggca aaactatctg ccacgataaa gcgtttgttc
     8521 gggcgctgcg caaagcgttc gaccttccgc atattaaaaa agccagttaa tatcatcagg
     8581 gagctaatcg gctccctttt tttaccttta attccgaaat ctttcgctgc atttgcgaat
     8641 tctgcttgtc tgattgcaga tgccaggtaa cataggtatc cccccattta cgggtggaca
     8701 agtgtttatt ttttccgact attaacagag agaatattat gagcgttgtg cctgtagccg
     8761 acgtactcca gggccgcgta gccgttgaca gcgaagtcac cgtacgcgga tgggtacgta
     8821 cccgccgaga ttcaaaagct ggcatctcct tcctcgccgt ttatgacggt tcctgctttg
     8881 atcctgtaca ggctgtcatc aataattctc tgcccaatta caatgaagac gtcctgcgtc
     8941 tgaccaccgg ctgctcggtc attgtgacgg gtaaagtcgt ggcgtcgccg ggccaggggc
     9001 aacaatttga aattcaggcc agcaaggttg aagttgctgg ttgggttgaa gatccagaca
     9061 cttacccgat ggcggcaaaa cgccacagca ttgagtatct gcgtgaagtc gctcacctgc
     9121 gtccgcgcac aaacctgatt ggtgccgtcg cgcgcgttcg tcatacgctg gcgcaggcgc
     9181 tgcatcgctt ctttaacgag cagggattct tctgggtttc aacgccactg attaccgcat
     9241 ctgataccga aggtgcaggc gaaatgttcc gcgtttctac gctggatctg gaaaacctgc
     9301 cgcgtaacga tcagggcaaa gtggatttcg acaaagactt ctttggtaaa gagtctttcc
     9361 tgaccgtatc tggccagttg aacggcgaaa cctacgcttg cgcattatcc aaaatttata
     9421 ccttcggccc gactttccgt gctgaaaact ccaacaccag ccgtcacctg gcggaattct
     9481 ggatgctgga gccggaagtg gcgtttgcta acctgaacga tatcgcgggt ctggctgaag
     9541 ccatgctgaa atatgtcttc aaagcggttc tcgaagaacg cgcagacgac atgaaattct
     9601 tcgctgaacg cgtagataaa gatgccgttt cacgtctgga acgcttcatt gaggccgatt
     9661 tcgcgcaggt ggattacacc gacgcagtga ccattctcga aaactgcggc aggaagtttg
     9721 aaaacccggt ttactggggc gtcgatctct cttctgagca tgagcgttat ctggcggaag
     9781 aacactttaa agcaccggta gtggttaaaa actatccgaa agatattaaa gcgttctata
     9841 tgcgcctcaa cgaagacggt aaaaccgttg cggctatgga cgttctggct ccgggcatcg
     9901 gtgagatcat tggtggctcc cagcgtgaag aacgtctgga cgttctggac gagcgtatgc
     9961 tggaaatggg cctgaataaa gaagattact ggtggtatcg cgatctgcgt cgctacggta
    10021 ctgttccgca ttcaggtttc ggtcttggtt ttgaacgcct gattgcttac gtaactggcg
    10081 tgcaaaacgt gcgtgatgtg attccgttcc cacgtactcc gcgcaacgcc agcttctgat
    10141 tttcttcctc aaagagccag cgcctgctgg ctctttttta tcctttctgt caagttatct
    10201 gtttgttaag tcaagcaatc tatttgcaac cccgccataa cgatcatcct gttacggaat
    10261 attacattgc aacatttacg cacaaaaaat aacccgcatt cttattgcgg attagttttt
    10321 tcttagctga tagcacaatt ttcatactat tttttggcat tctggatgtc tgaaagagga
    10381 ttttgtgcca gttcgataaa gtttccatca gaaacaaaat ttccgtttag ttaattta
//
LOCUS       DAZGTR010000146        10358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGTR010000146 DAZGTR010000000
VERSION     DAZGTR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.148"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            149..601
                     /locus_tag="ACHLCY_004167"
     CDS             149..601
                     /locus_tag="ACHLCY_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HFP0371309.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVARQHRHYGNATSARI"
     gene            687..1880
                     /locus_tag="ACHLCY_004168"
     CDS             687..1880
                     /locus_tag="ACHLCY_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0371310.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTRTADSRKPNKPVRKPLKSMIIDHSNDQHAGDHIAAEITEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGVPNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            1880..3364
                     /locus_tag="ACHLCY_004169"
     CDS             1880..3364
                     /locus_tag="ACHLCY_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0371311.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDLIIES
                     VQEQGSKTSVSSGHGTGKSDMTSIMIMLFIIMYPGARAIIVANKIQQVMTGIFKYIKI
                     NWATATSRFPWLADYFVLTETAFYEVTGKGVWTVVPKGFRLGSEEALAGEHADHLLYI
                     IDEASGVSDRAFGIITGALTGQDNRILLLSQPTRPSGYFYDTHHKLAKRPGNPDGVYT
                     AITLNSEESPLVTPAFIKMKLAEYGGRDNPMYMIKVRGLFPKSQDGFLLGRDEVERAT
                     RRKVKIAKGWGWLACVDVAGGTGRDKSVINIMMVSGQRNKRRVINYRMLEYTDVTETQ
                     LAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITVQRIRWGKKMHSREDKS
                     LYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGINSAGQWKVMSKEDMKKK
                     LNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWLNE"
     gene            3448..3678
                     /locus_tag="ACHLCY_004170"
                     /pseudo
     CDS             3448..3678
                     /locus_tag="ACHLCY_004170"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            3703..3924
                     /locus_tag="ACHLCY_004171"
     CDS             3703..3924
                     /locus_tag="ACHLCY_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0371312.1"
                     /translation="MATIPTPAHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            3921..5033
                     /locus_tag="ACHLCY_004172"
     CDS             3921..5033
                     /locus_tag="ACHLCY_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP0371313.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAEARKEAHRMKATNVKSEKIHAPSVQES
                     EMVVVQNQSDEIPVLEWQGVRVVTTETLAKGYGTDEGNIRKNLSCNSDRFEEGKHYFL
                     LTGSKLKEFKNRVTFSNSVGKNARNLTLWTERGAARHAKMIETDQAWAFFEKLEDSYF
                     RQKEQQPVAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSVA
                     KGILIGNFAKVVGLKQNALFVWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLDD
                     EDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(5066..5917)
                     /locus_tag="ACHLCY_004173"
     CDS             complement(5066..5917)
                     /locus_tag="ACHLCY_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein C1"
                     /protein_id="HFP0371314.1"
                     /translation="MINYVYGEQLYQEFVSFRDLFLKKAVARAQHVDAASDGRPVRPV
                     VVLPFKETDSIQAEIDKWTLMARELEQYPDLNIPKTILYPVPNILRGVRKVTTYQTEA
                     VNSVNMTAGRIIHLIDKDIRIQKSAGINEHSAKYIENLEATKELMKQYPEDEKFRMRV
                     HGFSETMLRVHYISSSPNYNDGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSL
                     YDSIEPIICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVN
                     DNPESAQ"
     gene            complement(6028..6237)
                     /locus_tag="ACHLCY_004174"
     CDS             complement(6028..6237)
                     /locus_tag="ACHLCY_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c1 repressor inactivator"
                     /protein_id="HFP0371315.1"
                     /translation="MAFIPPTIDDVRHCSNALSVDPAETDAARAIAEHYSKISNQEYR
                     ITQDDLDDLTDTIEYLMATNQPDSQ"
     gene            6842..7063
                     /locus_tag="ACHLCY_004175"
     CDS             6842..7063
                     /locus_tag="ACHLCY_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HFP0371316.1"
                     /translation="MGYSAAKVSTHIELEKNRGYWRAKGFDRDSCQLSLSRGEEKIER
                     TRGRWRFYDENHKQVKAEPILYTLLKTII"
     gene            7071..8102
                     /locus_tag="ACHLCY_004176"
     CDS             7071..8102
                     /locus_tag="ACHLCY_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0371317.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNILHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRN
                     LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVASPSPTSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLENGN"
     gene            8153..8464
                     /locus_tag="ACHLCY_004177"
     CDS             8153..8464
                     /locus_tag="ACHLCY_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HFP0371318.1"
                     /translation="MRKDFNIDGKYVVLSVSTNILSPSVIVTVKLSDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGLTRVIGEFGELLGKVNNALSISSARSKALTASMM
                     K"
     gene            complement(8705..9820)
                     /locus_tag="ACHLCY_004178"
     CDS             complement(8705..9820)
                     /locus_tag="ACHLCY_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP0371319.1"
                     /translation="MRTSLVTREEMIEAIEQHTACISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATIAAEARKEAHRMKATNVKSEKIHATSVQKS
                     ELVVDQNQPDEIPVLEWLGVRVVTTKILAKGYGTDADNIKKNFSRNAERFCEGKHFYK
                     VIGDDLNNLRVTLSNSQNPISPKARSLILWTERGAARHAKMLETDQAWAFFEKLEDSY
                     FRQKEQQPIAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVSV
                     ANGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVLD
                     DEDGYQIRLTPQLTGKGQQWLTRKLLEAGLLKPVAAE"
     gene            complement(9817..10038)
                     /locus_tag="ACHLCY_004179"
     CDS             complement(9817..10038)
                     /locus_tag="ACHLCY_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0371320.1"
                     /translation="MVNANPCARPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPC
                     IFSARFSTDLRNSLSYWSLPVNASAQEGL"
     gene            complement(10154..>10358)
                     /locus_tag="ACHLCY_004180"
     CDS             complement(10154..>10358)
                     /locus_tag="ACHLCY_004180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371321.1"
                     /translation="LIYWGKNTTIERQTIDLPVVQAYSSTVVANSATGFSSLNTNADN
                     RRYPILRYFCVRKTTLRPNYGGA"
BASE COUNT         2721 a         2363 c         2604 g         2670 t
ORIGIN      
        1 ttctttgatt acgcgcttgg agtcggcgct atcacaagtc actcgtcgag cgttagataa
       61 aattgctcta atgtattgct atttctttaa tcgagggtat tatattccac gttgattagt
      121 tgacatgggc taatcagtag gtgacaggat gttacttaac tggcagggac gccacttcat
      181 ggaaataaat cactcacgaa taacatcgta cgagattgcg gattacatga tccgcactaa
      241 atctcttcta tcagcgaaag aactcgcagc aattcttgaa aaggaatacc cgcatctgga
      301 tgtcgataag cgcgatgttt atctgcgctt aaaggctatc gctgtgtcta agtattcgtc
      361 tgttttgatt gatgacagta cacgcccacg tagatttcag atccactctc tgaatcctga
      421 attctttcgc cgtagccgcg ctccgcgccg gtttgatgaa aaactccaga acgaactcta
      481 tatgacgcag gacgaaaagg aacgccggga gcaccagcct tgggtaatgg cgcgtcaact
      541 tttcaataag gtggcccgtc agcaccgtca ttacggtaat gccacatccg cacgtatctg
      601 attgattgct tgcccgttcc gggccttttg acatgtgact ttcgttaccc tcgcgtcaaa
      661 aagagttttt acgaaaggaa gcataagtga cctgggacga tcacaagaag aattttgctc
      721 gcctggcgcg agatggtggt tacaccatcg cacagtatgc cgccgagttt aatcttaacc
      781 ctaatactgc acgtcgttat ctccgtgcct tcaaagaaga caccaggaca gcggacagcc
      841 gcaagccaaa taagccagtc aggaagccac taaaaagcat gatcattgat cactctaatg
      901 atcaacatgc aggtgatcac attgcggctg aaataacgga aaaacaaagg gttaatgccg
      961 ttgtcagtgc cgcagtcgag aatgcgaagc gccaaaataa gcgcataaat gatcgttctg
     1021 atgatcatga cgtgatcacc cgcgcccacc ggaccttacg tgatcgcctg gaacgcgaca
     1081 ccctggatga tgatggtgaa cgctttgaat tcgaagctgg cgattacctg atagataacg
     1141 ttgaagcgcg gaaggccgcg cgcgctatgt tgcgtcggtc cggggccgat gttctggaaa
     1201 ccactcttct ggaaaagtct ctttctcatc tccttatgct ggagaacgcc agggatacgt
     1261 gtattcgtct ggtgcaggaa atgcgcgatc agcaaaaaga cgatgatgaa gggactccgc
     1321 ctgaatatcg tatcgcgagc atgctaaaca gctgttccgc gcagataagc agcctgatca
     1381 acaccattta cagcatccgg aataactatc gaaaagaaag ccgggaggcg gaaaagcacg
     1441 ctttatctat ggggcaagct ggcattgtta agctggcata cgaacgaaag cgtgaaaata
     1501 actggtcagt gctggaagca gctgaattca tcgaggcgca tggcggaaaa gtgccgcccc
     1561 tgatgctgga gcaaatcaaa gccgatctgc gtgctcctaa gaccaatacc gatgatgagg
     1621 aaaggcaaac agccgtcggt ggcccttctc ttgaagatct ggacaaagtt gcgcgagaac
     1681 gggccgccaa ccgccgcgcc gatgccgcat tgtggattga gcagcgtagg gaagaaatcg
     1741 ccgatatcgt tgatacaggc ggttatggag atgttgatac tgaaggtgta ccaaacgacc
     1801 catggctgga acaagacctg gacgaagacg aggaggaaga cgaagaagtt acccgcaagc
     1861 tatacgggga tgatgattaa tggccagaag ttgcgtaacg gatccacgtt ggcgcgagct
     1921 ggtggcgcta tatcgttatg actggattgc tgccgctgat gttttgttcg gcaaaacacc
     1981 tacctggcag caggatctga ttattgagtc tgtgcaggaa cagggtagca agacatctgt
     2041 ttcgtctggt cacggtaccg ggaaatcaga catgacttct atcatgatca tgttgttcat
     2101 aatcatgtat cccggtgccc gcgccattat cgttgcgaac aaaattcagc aggtaatgac
     2161 cggtatattc aagtacatca agataaactg ggctactgcc accagccgtt ttccatggct
     2221 tgctgattat tttgttctga cagaaaccgc tttctatgag gttactggta aaggtgtatg
     2281 gactgtagta ccgaagggct ttcgtctggg aagtgaagaa gctctcgccg gtgaacacgc
     2341 agatcatctt ctgtatatta tcgatgaagc ctccggtgtc agtgatagag ctttcggtat
     2401 catcaccggt gctcttaccg gacaggataa ccgcatctta ttactgtcac agcctacacg
     2461 cccaagcggc tatttctacg atactcacca taaactggcc aagcgtcctg gtaaccctga
     2521 tggcgtttat acggcgatca cgcttaacag tgaggaatca ccgctggtaa cgccagcatt
     2581 tatcaaaatg aagctggcgg agtacggcgg gcgtgataac cctatgtaca tgattaaggt
     2641 acgcggccta ttccctaaat cacaggatgg cttccttctt ggacgtgatg aggttgaacg
     2701 tgcaacgcgg cggaaagtca agattgcaaa aggatggggc tggcttgcat gtgtggacgt
     2761 tgctggtggt acgggacggg ataagtccgt tatcaatatc atgatggtgt ccggccagcg
     2821 aaataaacgc cgtgtaatca actatcgaat gctggaatac acagacgtta cagaaacgca
     2881 gcttgccgcc aaaattttcg cagaatgtaa tcctgagcga ttcccaaata tcaccatagc
     2941 gatagacggc gatggcctgg gtaaagcaac ggcggatctg atgtacgagt attatggtat
     3001 taccgtacag cgtatacgct ggggtaaaaa gatgcatagc cgtgaagata agagcctgta
     3061 ctttgataaa cgtgcttatg ccaacgttca agccgcagag gccgtaaaat ctggtcgtat
     3121 gagactggat aagggtaatg aaactattga ggaagcgtcg aaaatccctg tagggattaa
     3181 ctccgcaggt caatggaagg tgatgagtaa ggaggatatg aagaaaaaac tcaacctgca
     3241 ctcaccagac cattgggata catattgttt cgctatgctg gcggattatg ttccccagga
     3301 tgaagtgctt agcgtcgaag acgaagcgca ggttgatgaa gctctggcat ggcttaatga
     3361 atgaatattt gctctaataa attgtgtttt ttaactgccg atgttacatt gaacctgacc
     3421 tcttgcgcct tgaggcattt tcggtttatg cttatcaggc acctcattaa aacgggtgcc
     3481 gggattggcc tcccgcttaa gtctaaggcg atacagacgc cgctcgcgtc tttttttgta
     3541 tcggcgtaca cgcacacctc tacaatggtg ggctgtatgg ggctaccttc gggtaggctg
     3601 gttaccttgg acgccagtaa ggccaactcc gtacagtcca ccgccagcaa gattggtctc
     3661 ttctgcggtg gttacatacc aacgtctaag gaggctgcca atatggctac tatccctacc
     3721 cctgctcatc ctgaatttat ctggcgcttt tactcctgcc agaaacgtca ctatcacttc
     3781 gtaattgcac cgacagaaga tgaggcccgc tctcagcttc ctgacgcccc atgtattttc
     3841 tctgcccgtt tttccactga ttcacgcaat tctctcagtt actggtgcct ccctgttaac
     3901 gcttctgctc aggagggact atgagaacgt cattagtcac ccgtgaagag atgatcgagg
     3961 caattgaaca gcacactgcc tgtatcagta ccagggatat accaggcgtt attgccaact
     4021 acttcatgat caccaaacaa ctttaccgga gaaaggacaa gaacgcggtt caccgtatcc
     4081 tgttgtctga tatccgcgaa tacctgctcg aacagggtca tctgaattac gcaaccgtcg
     4141 cagccgaagc acgcaaggag gcacacagaa tgaaagcaac taacgttaaa tcagaaaaaa
     4201 ttcatgcacc ttcagttcag gaatcggaga tggtggttgt tcagaatcag tctgatgaaa
     4261 ttcccgttct ggaatggcag ggagtacgtg tcgtaacaac cgaaactctt gctaaggggt
     4321 acggaaccga tgaaggcaat attcgtaaaa atttgtcttg caactccgat cgctttgaag
     4381 aaggtaaaca ctactttctt ttaactggtt caaaattaaa ggaatttaag aacagagtta
     4441 cttttagtaa ctctgttggt aagaacgcca gaaacctcac tctctggacg gaacgcggcg
     4501 cagcccgcca cgctaaaatg atcgaaaccg atcaggcatg ggcattcttt gaaaaactgg
     4561 aagacagcta cttccggcaa aaagaacagc aaccggtcgc aatccctcag acgcttcccg
     4621 aagctctgcg cctggctgcc gagctggctg aacaaaagca acttctggaa cagaaagccc
     4681 accagctaaa tcagcagctg gtggccgccg cccctaaggt cgattttgcc gaccgggtat
     4741 cagtggctaa ggggatcctg attgggaatt ttgcaaaggt tgttggactt aagcaaaacg
     4801 cgctgtttgt ctggttacgg gagaacggca tcctgatagc gtccggtgga cgtaaaaatg
     4861 tgccgttcca gcagtacatc aacgcggggt atttcacggt gaaagaagtg gtgctggatg
     4921 atgaagatgg ctaccagata cggttgacgc ctcaattaac gggtaaaggc cagcagtggt
     4981 tgacgcgtaa actgctcgat gctggcttgt taaaaccggt ggcagctgaa taatggaaga
     5041 atgcccggtt gatgccgggc ataatttatt gcgcgctttc ggggttgtcg tttactggct
     5101 gccccttctt ggttttacgg ctgcgcgtaa ctgatgcggc tgacttaacc tttttctctt
     5161 cgcgagtgat ggcaatttgt ttttttacat tttcaatatc tgccaggcga tatatttttg
     5221 cttgcggcca gcggtcgcag atgatcggtt ctatggagtc ataaaggcta aattttgctt
     5281 tttcgaattc accgttgatg ataattccat cacggagagt ttcatcgcag ataaacacgc
     5341 cacacagtgg cacatggtaa ctaactgatt taccatcatt gtagttaggg ctactagaaa
     5401 tgtaatggac gcgcagcatt gtttcgctaa agccgtgtac gcgcatacgg aatttttcat
     5461 cctccgggta ctgcttcatt agctcttttg ttgcttccag gttctctatg tatttcgcac
     5521 tgtgctcatt gatccctgcg cttttctgga tgcgaatgtc cttatcaatc agatgaataa
     5581 tgcggccagc ggtcatgttg acgctgttca cagcttctgt ctgataagtc gtaaccttac
     5641 gcacaccgcg aaggatgtta ggcactggat ataaaatagt ctttgggata ttgaggtctg
     5701 ggtactgttc cagttcccgc gccattaaag tccatttatc aatttcagcc tgaatgctgt
     5761 cagtttcttt gaacggcaga acgacaaccg ggcgtacagg acgaccgtcg ctggcggcat
     5821 caacgtgttg ggcgcgtgca acagcttttt ttagaaagag atccctgaag ctgacgaact
     5881 cctggtacag ttgttcgccg tagacataat ttatcattga tcctcctcca gaattgacat
     5941 ggccaacaac tcacagcgga ttacactggg agttgttggc caccattata gaaggatcca
     6001 acgaaaataa tagatttatt agtgcattta ttgtgagtct ggctggttag tggccatgag
     6061 atattcgatt gtgtcagtga gatcatccag gtcgtcttgg gtgatgcggt actcctgatt
     6121 ggatatcttt gagtagtgtt cagcaatggc gcgggcagcg tcggtttcgg cggggtctac
     6181 agataaagcg ttagagcaat gtctaacgtc gtcgatggtt ggtggaatga aagccataat
     6241 tatgcctcac tgtattgaca acacagagcc tgaagctctg acctactgtt tcacccatga
     6301 tccatgctgg ggtaatctaa caacattgcg ctgtgtgtaa gatgagcaat gcatagctgt
     6361 aatgccgttg tataaggttt ccctgtttgc tcatttcctt ctgagccgct ctacaacgct
     6421 gaagacacat taaatagtga atccaaagtc gtattacgaa acggcggcaa aactataatt
     6481 tattagagca attgtcaaac aactatgaaa aacaatccag tttttggctg gtggagtggg
     6541 atttttctct caaaatttat tgctctaata attcttgatt tttatgcgca gctggacgta
     6601 aactcctctt cagacctaat aacttcgtat agcatacatt atacgaagtt atcttaaggg
     6661 ttattgaaca tgatcaattt acctgtaaat ccatacagtt caatacctta tcaggtcaaa
     6721 tagtgatcac ttgatcattt gatcaaggtt gcgctacgta aaatctgcga aatgttggca
     6781 gtgttagtgc tccagatttc gcgtagcgca cttagcacca ccaatcaatc agaggtgaaa
     6841 aatgggatat tcagctgcta aagtgtccac tcatattgag cttgagaaaa accgtggtta
     6901 ctggcgggca aaagggtttg atcgtgatag ttgccaactg tcattatcgc gcggtgaaga
     6961 gaaaatagaa cgcacgcgcg gtcgctggcg tttctatgac gagaaccata aacaggtaaa
     7021 ggcagagccg atcctgtaca ctttacttaa aaccattatc tgagtgttaa atgtccaatt
     7081 tactgaccgt acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc
     7141 gcaagaacct gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa
     7201 tgcttctgtc cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt
     7261 ttcccgcaga acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg
     7321 cagtaaaaac tatccagcaa catttgggcc agctaaacat acttcatcgt cggtccgggc
     7381 tgccgcgacc aagtgatagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa
     7441 acgttgatgc cggtgaacgt gcaaaacagg cactagcgtt cgaacgcact gatttcgacc
     7501 aggttcgttc actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc
     7561 tggggattgc ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag
     7621 atatctcacg tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg
     7681 ttagcaccgc aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat
     7741 ggatttctgt ctctggtgtg gctgatgatc cgaataacta cctgttttgc cgggtcagaa
     7801 aaaatggtgt tgcctcgcca tcacccacca gccagctatc aactcgcgcc ctagaaggga
     7861 tttttgaagc aactcaccga ttgatttacg gggctaagga tgactctggc cagaggtacc
     7921 tggcctggtc tggacacagt gcccgtgtcg gagccgcgcg agatatggca cgtgccggag
     7981 tttcaatacc ggagatcatg caagctggtg gctggaccaa cgtaaatatt gtcatgaact
     8041 atatccgtaa cctggatagt gaaacagggg caatggtgcg cctgctggaa aatggcaatt
     8101 agccattaac gcgtaaatga ttgctctaat tctttgatat ttatggtggc atatgagaaa
     8161 ggatttcaac atcgacggaa aatatgtagt gctgtctgta agcactaata ttctgtcgcc
     8221 atctgtcatt gtcaccgtaa agttgagcga taggatgcct gatatcgact cgatatctgt
     8281 tgcgttcccc gttaaaagca tgcggagtgc tgaacatttt gtgatgaatg caacggagga
     8341 ggaagcgcgg cgcgggctta ctagagtgat aggggaattt ggcgaactcc tgggtaaggt
     8401 aaacaatgcc ctttcaatca gttcagcaag gtccaaagcg ttaacagctt ccatgatgaa
     8461 ataaaaaaaa gcctggcaag gagccaggct gcataaaaga gcgggtttgt attccgcatc
     8521 caatcaatca agaaggagta tagcacacag gtactgaaga gaaaaaatgt gattcgcgat
     8581 aaaaaataaa atctcattac tctaattgat tgttataatt aagaggtggc gtgagtcaac
     8641 tacgaacaca tggccattac ctgcttcttg aaatcattgg cggcctaaag gccgcctttt
     8701 ttaatcattc agccgccacc ggttttaaca agccagcttc gagcagttta cgcgttaacc
     8761 actgttggcc tttccccgtt aattgaggcg tcaaccgtat ctggtagcca tcttcatcat
     8821 caagcaccac ttctttcacc gtgaaatacc cggcgttgat gtattgctgg aacggtacgt
     8881 ttttgcgtcc accggacgct atcaggatgc cgttctcccg taaccaggca aacagcgcgt
     8941 tttgcttaag tccaacaacc tttgcaaagt tcccgatcag gattccattg gccactgata
     9001 cccggtcggc aaaatcgact ttaggggctg cggccaccag ctgctgattt agctggtggg
     9061 ctttctgttc cagaagctgc ttttgttcag ccagttcggc agccaggcgc agagcttctg
     9121 gaagcgtctg ggggattgcg atcggttgct gttctttttg tcggaagtag ctgtcttcca
     9181 gtttttcaaa gaatgcccat gcctgatcgg tttcgagcat tttagcgtgg cgggctgcgc
     9241 cgcgttccgt ccagaggatg agtgagcggg ctttaggaga gatggggttt tgtgagttac
     9301 ttaaagtcac ccgcaaattg ttcaaatcat ctccaataac tttatagaaa tgcttgcctt
     9361 cacaaaaacg ttcagcatta cgggaaaagt tcttcttgat gttatccgca tcggtcccat
     9421 accccttagc aaggatcttg gttgtcacta cacgcactcc cagccattcc agaacgggaa
     9481 tttcatccgg ctgattttga tcaaccacca gctccgattt ctgaactgaa gttgcatgaa
     9541 ttttttctga tttaacgtta gttgctttca ttctgtgtgc ctccttgcgt gcttcggctg
     9601 cgatggttgc gtaattcaga tgcccctgtt cgagcaggta ttcgcggata tcagacaaca
     9661 ggatacggtg aaccgcgttc ttgtcctttc tccggtaaag ttgtttggtg atcatgaagt
     9721 agttggcaat aacgcccggt atatccctgg tactgataca ggcggtatgc tgttcaattg
     9781 cctcgatcat ctcttcacgg gtgactaatg acgttctcat agtccctcct gagcagaagc
     9841 gtttacaggg aggctccagt aactgagaga attgcgcaaa tcagtggaaa aacgggcgga
     9901 gaaaatgcag ggagcatccg gaagctgaga gcgggcctca tcttctgtcg gtgcaataac
     9961 gaagtgatag tgacgtttct ggcaggagta aaagcgccag atgaattctg ggcgtgcgca
    10021 aggattggca ttaaccatag ttacggcctc attcgtaggt ttaacaacct gcgccccgct
    10081 gctaaacggg tggcaggacg tgacggggtt agcagactgg cacgaatgaa accagcaggc
    10141 cgaagcctcc ccatcacgcc ccaccataat tcgggcgtaa cgtggtttta cggacacaaa
    10201 aataccgcaa tatcggatat ctgcggttgt ccgcattcgt attcaggctg ctaaacccgg
    10261 tcgcagaatt tgctacgacg gtggaactat aagcctgaac gacaggaaga tcaatagtct
    10321 ggcgttcaat ggtagtattt ttgccccaat aaatcaaa
//
LOCUS       DAZGTR010000147        10050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGTR010000147 DAZGTR010000000
VERSION     DAZGTR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.149"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1967
                     /gene="acnA"
                     /locus_tag="ACHLCY_004181"
     CDS             <1..1967
                     /gene="acnA"
                     /locus_tag="ACHLCY_004181"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP0371322.1"
                     /translation="LGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVV
                     GKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVEL
                     VEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASN
                     ELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAV
                     TLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPD
                     PIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAE
                     PIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSD
                     TYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDS
                     PAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDS
                     DVVSIYDAAMRYKKEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHR
                     SNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVV
                     PCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2031..2621)
                     /gene="ribA"
                     /locus_tag="ACHLCY_004182"
     CDS             complement(2031..2621)
                     /gene="ribA"
                     /locus_tag="ACHLCY_004182"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP0371323.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2791..3555
                     /gene="pgpB"
                     /locus_tag="ACHLCY_004183"
     CDS             2791..3555
                     /gene="pgpB"
                     /locus_tag="ACHLCY_004183"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP0371324.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPEEENREIAQREQES"
     gene            3704..4012
                     /gene="lapA"
                     /locus_tag="ACHLCY_004184"
     CDS             3704..4012
                     /gene="lapA"
                     /locus_tag="ACHLCY_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP0371325.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4019..5188
                     /gene="lapB"
                     /locus_tag="ACHLCY_004185"
     CDS             4019..5188
                     /gene="lapB"
                     /locus_tag="ACHLCY_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP0371326.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5380..6117
                     /gene="pyrF"
                     /locus_tag="ACHLCY_004186"
     CDS             5380..6117
                     /gene="pyrF"
                     /locus_tag="ACHLCY_004186"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP0371327.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6117..6443
                     /gene="yciH"
                     /locus_tag="ACHLCY_004187"
     CDS             6117..6443
                     /gene="yciH"
                     /locus_tag="ACHLCY_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP0371328.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6569..6787)
                     /gene="osmB"
                     /locus_tag="ACHLCY_004188"
     CDS             complement(6569..6787)
                     /gene="osmB"
                     /locus_tag="ACHLCY_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP0371329.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(7056..7805)
                     /gene="yciT"
                     /locus_tag="ACHLCY_004189"
     CDS             complement(7056..7805)
                     /gene="yciT"
                     /locus_tag="ACHLCY_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP0371330.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSRIRASDLMHLEHSKLTVHVVDI"
     gene            complement(7895..8068)
                     /gene="yciZ"
                     /locus_tag="ACHLCY_004190"
     CDS             complement(7895..8068)
                     /gene="yciZ"
                     /locus_tag="ACHLCY_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP0371331.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            8106..8234
                     /gene="ymiD"
                     /locus_tag="ACHLCY_004191"
     CDS             8106..8234
                     /gene="ymiD"
                     /locus_tag="ACHLCY_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP0371332.1"
                     /translation="MQKLPLKEKCLTATANYHPRIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(8216..10050)
                     /gene="pdeR"
                     /locus_tag="ACHLCY_004192"
                     /pseudo
     CDS             complement(8216..10050)
                     /gene="pdeR"
                     /locus_tag="ACHLCY_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
BASE COUNT         2545 a         2378 c         2569 g         2558 t
ORIGIN      
        1 tgttaggcca gccggtttcc atgcttatcc cggacgtagt gggctttaaa cttaccggta
       61 aattacgtga aggtattacc gccacagacc tggttctcac tgttacccaa atgctgcgca
      121 aacatggcgt ggtggggaaa ttcgtcgaat tttatggtga tggtctggat tcactaccgc
      181 tggcggatcg cgccaccatt gccaatatgt cgccagaata tggtgccacc tgtggcttct
      241 tcccaatcga tgctgtaacc ctcgattaca tgcgtttaag cgggcgcagc gaagatcagg
      301 ttgagttggt cgaaaaatat gccaaagcgc agggcatgtg gcgtaacccg ggcgatgaac
      361 cgatttttac cagtacgtta gaactggata tgaatgacgt tgaagcgagc ctggcagggc
      421 caaaacgccc acaggatcgc gttgcactgc ccgatgtacc aaaagcattt gccgccagta
      481 acgaactgga agtgaatgcc acgcataaag atcgccagcc ggtcgattat gttatgaacg
      541 gacatcagta tcagttacct gatggcgctg tggtcattgc tgcgataacc tcgtgcacca
      601 acacctctaa cccaagtgtg ctgatggccg caggcttgct ggcgaaaaaa gccgtaactc
      661 tgggcctcaa gcggcaaccg tgggtcaaag cgtcgctggc accgggttcg aaagtcgttt
      721 ctgattatct ggcaaaagcg aaactgacac cgtatctcga cgaactgggg tttaaccttg
      781 tgggatacgg ttgtaccacc tgtattggta actctgggcc gctgcccgat cctatcgaaa
      841 cggcaatcaa aaaaggcgat ttaaccgtcg gtgcggtgct gtccggcaac cgtaactttg
      901 aaggccgtat ccatccgctg gttaaaacta actggctggc ctcgccgccg ctggtggttg
      961 cctatgcgct ggcgggaaat atgaatatca acctggctgc tgagcctatt ggtcatgatc
     1021 gtaaaggcga cccggtttat ctgaaagata tctggccatc ggcacaagaa attgcccgtg
     1081 cggtagaaca agtctccaca gaaatgttcc gcaaagagta cgcagaagtt tttgaaggca
     1141 cagcagagtg gaaggaaatt aacgtcacac gatccgatac ctacggttgg caggaggact
     1201 caacctatat tcgcttatcg cctttctttg atgaaatgca ggcaacacca gcaccagtgg
     1261 aagatattca cggtgcgcgg atcctcgcaa tgctggggga ttcagtcacc actgaccata
     1321 tctctccggc gggcagtatt aagcccgaca gcccagcggg tcgatatcta caaggtcggg
     1381 gtgttgagcg aaaagacttt aactcctacg gttcgcggcg tggtaaccat gaagtgatga
     1441 tgcgcggcac cttcgccaat attcgcatcc gtaatgaaat ggtgcctggc gttgaagggg
     1501 ggatgacgcg gcatttacct gacagcgacg tagtctctat ttatgatgct gcgatgcgct
     1561 ataagaagga acaaacgccg ctggcggtga ttgccgggaa agagtatgga tcaggctcaa
     1621 gccgtgactg ggcggcaaaa ggtcctcgtc tgcttggaat tcgtgtggtg attgccgaat
     1681 cgtttgaacg aattcaccgt tcgaatttaa ttggcatggg catcctgccg ctggaatttc
     1741 cgcaaggcgt aacgcgtaaa acgttaggac taaccgggga agagaagatt gatattggcg
     1801 atctgcaaaa tctacaaccc ggtgcgacgg ttccggtgac gcttacgcgc gcggatggta
     1861 gccaggaggt tgtaccctgc cgttgtcgta tcgacaccgc gacggagttg acctactacc
     1921 agaacgacgg cattttgcat tatgtcattc gtaatatgtt gaagtaacaa gtatttgctt
     1981 gccggttatt ttgcttccgg caagcaaatt aattacacaa tgcaagaggg ttatttgttc
     2041 agcaaatggc ccattttctc ggctttggta tcgagataat gttcgttatt ggggttacga
     2101 cctacaatca atggtacgcg ttcaacaata ttaatccctg cttcggtcag aatttcgact
     2161 tttttcgggt tattggttaa caagcggact tcattgacgc caaggagttt gaacatatca
     2221 gcgcaaagag tgaagtcgcg ctcatcagcg gcgaagccta actggtggtt agcctctacg
     2281 gtatcgtaac cttgatcctg cagtgcgtaa gcgcggattt tattcagcag accaatgtta
     2341 cgaccttcct gacggtgata cagcaaaata ccacggcctt cctcggcaat ttgcgtcaat
     2401 gccgcttcga gctggaagcc acaatcgcag cgcaagctga acagggcgtc accggtcaga
     2461 cattcggaat ggacgcgcgc aagtaccggg gtatgcccgg aaatatcgcc atagactagc
     2521 gcgacatgat cgtgtccggt tgccagttct tcaaatccca ccatcaggaa atcgccccat
     2581 ggggttggca gtttggcttc tgccacacgt ttaagctgca tgaaattctc cagataatgc
     2641 tggttctgta ttggcttatt ttgccataac gagaaggggt tcacctaatc acgacgcgtc
     2701 gatcgttcac ggaatggcac aaatctgtca atttttcctg gaactggcgt tttcagttat
     2761 gattgtggga cttatcaaaa aggagaggcc atgcgttcga ttgccagacg taccgcagtg
     2821 ggagctgcac tattgcttgt catgccagta gccgtatgga tttctggctg gcgttggcaa
     2881 cctggagaac aaagttggct actaaaagcg gctttttggg ttactgaaac tgtcacccag
     2941 ccctggggcg tcattacaca tttgatttta ttcggctggt ttctctggtg tctgcgtttt
     3001 cgcattaagg ctgcctttgt attatttgcc attctggcgg ccgcaatcct tgtgggacaa
     3061 ggcgttaaat cctggatcaa agacaaagtc caggaaccac gaccttttgt tatctggctg
     3121 gaaaaaacac atcatattcc ggttgatgag ttctacactt taaagcgagc agaacgcgga
     3181 aatctagtga aagaacagtt ggctgaagag aaaaatatcc cacaatattt gcgttcacat
     3241 tggcagaaag agacggggtt tgcctttcct tccggtcaca cgatgtttgc tgccagttgg
     3301 gcactgctgg ccgttggttt gctgtggccg cgtcggcgaa cgttaaccat tgctatcttg
     3361 ctggtctggg caacgggagt catgggaagc cgcctgctgc tcgggatgca ttggccacgc
     3421 gatctggtag tagctacgtt gatttcgtgg gcgctggtgg cggtggcaac gtggcttgcg
     3481 caacgaattt gtgggccatt aacaccacct gaggaagaaa atcgcgaaat agcgcaacga
     3541 gaacaagaaa gttaacgctg gttgattttc cgaatttagc ccttaaatca tcaacaatgc
     3601 gcgtggatgc catttcgcag acggcgcgaa aatggtactt taaagggcta ttgcggtaag
     3661 ttgaccataa tttattcgct ctaaccacat aacgggaagt aatgtgaaat atttactcat
     3721 tttcttactg gtgttagcga tcttcgtgat ttcggtcacg ttgggtgcgc agaacgatca
     3781 acaggtgacg tttaattatc tgttagcgca aggggagtac cgtatttcca cattgctggc
     3841 ggtattgttt gctgcggggt ttgctatcgg ttggttgatt tgtggcctgt tctggctgcg
     3901 agttcgtgtt tccctggcgc gcgctgaacg taaaataaag cgactggaaa accagctttc
     3961 acccgcgact gacgtggctg tagtgccgca ctcgtcagcg gcgaaggaat aactttctat
     4021 gctggagttg ttgtttctgc ttttgcctgt agccgctgcc tatggctggt atatgggccg
     4081 cagaagtgcg caacaaaaca agcaagatga agccaaccgc ttgtcgcgtg attacgtagc
     4141 gggggttaac ttcctgctta gtaatcaaca ggataaagcg gtagacctgt ttctcgatat
     4201 gcttaaagag gatacaggca ccgttgaagc ccaccttacg ctcggaaacc tgttccgttc
     4261 gcgtggcgaa gttgatcgcg ctattcgcat ccatcagacc ctaatggaaa gcgcctcgct
     4321 gacctatgaa cagcgtctgt tggcgattca acaactaggg cgtgattaca tggccgccgg
     4381 gttatacgac cgcgcggaag acatgttcaa tcagctgacc gatgaaactg acttccgcat
     4441 tggcgctctg caacagttgc tacaaatcta ccaggctacc agtgagtggc agaaagcaat
     4501 tgatgttgcc gaacgcctgg tgaagctggg taaagataaa cagcgcgtcg aaattgccca
     4561 tttctactgt gagttagccc tgcagcatat ggccagcgac gatctcgatc gtgccatgac
     4621 cttgctaaaa aaaggggcgg cggcagataa aaacagcgcc cgcgtatcca tcatgatggg
     4681 acgcgtgttt atggcgaaag gagaatacgc caaagccgtc gaaagtttgc aacgcgtgat
     4741 atcccaggac agagaactgg tcagcgaaac gctggaaatg ttgcaaacct gctatcagca
     4801 gttgggtaaa actgccgaat gggcagaatt cctgcaacgc gcggtggaag agaacaccgg
     4861 tgccgatgct gaattgatgc ttgctgatat catcgaagcg cgcgacggta gtgaggccgc
     4921 acaggtctat attacgcgcc agcttcagcg tcatccgacc atgcgtgtgt tccataagtt
     4981 aatggattac cacttaaatg aagcggaaga agggcgtgcc aaagagagcc tgatggtgct
     5041 gcgtgacatg gttggcgaaa aggtacgtag taagcctcgt tatcgctgcc agaaatgtgg
     5101 ttttaccgca tacaccctct actggcattg tccgtcttgt cgggcctggt caaccattaa
     5161 accgattcgc ggtcttgatg gcctgtaatt tttaaaaaat cctactttag ttacaacata
     5221 ctagttattg atgttccatt gtgctccggc aacgacggcg cagaaaaagc ctgccagggg
     5281 aaaaatcgca actgttaatt ttttatttcc acgggtagaa tgctcgccgt ttacctgttt
     5341 cgcgccactt ccggtgccca tcatcaagaa ggtctggtca tgacgttaac tgcttcatct
     5401 tcttcccgcg ctgttacgaa ttctcctgtg gttgttgccc ttgattatca taatcgtgat
     5461 gacgcgctgt cctttgtcga caagatcgac ccacgcgatt gtcgtctgaa ggtcggcaaa
     5521 gagatgttta cattgtttgg gccacagttt gtgcgcgaac ttcaacagcg tggttttgat
     5581 atctttcttg acctgaaatt ccacgatatt cctaacactg ctgcgcacgc tgtcgctgct
     5641 gcagctgact taggcgtgtg gatggtgaat gttcatgcct ctggtggggc gcgtatgatg
     5701 accgcagcgc gtgaggcact ggttccgttt ggcaaagatg caccgctttt gattgctgtg
     5761 acagtgttga ccagcatgga agccagcgac ctggtcgatc ttggcatgac actgtcacct
     5821 gcagattatg cagaacgtct ggcggcactg acgcaaaaat gtggccttga tggtgtggtg
     5881 tgttctgctc aggaagctgt gcgctttaaa caggtattcg gtcaggagtt caaactggtt
     5941 acgccgggca ttcgtccgca ggggagtgaa gctggtgacc agcgccgcat tatgacgcca
     6001 gaacaggcgt tgtcggctgg tgttgattat atggtgattg gtcgcccggt aacgcaatcg
     6061 gtagatccag cgcagacgct gaaagcgatc aacgcctctt tacagcggag tgcatgatga
     6121 gtgattccaa cagccgtctg gtctactcaa cggaaaccgg acgtattgat gagcccaaag
     6181 cggcccctgt acgccctaaa ggcgacggtg tggtgcgtat tcagcgtcag accagtggac
     6241 gtaaaggtaa gggcgtttgc ctgattaccg gtgtcgatct cgatgatgcc gaactgacaa
     6301 aacttgcagc ggaactgaag aaaaaatgcg gctgcggcgg agcagttaaa gatggagtta
     6361 ttgaaatcca gggcgataag cgtgatttat taaaatcact acttgaagcg aaagggatga
     6421 aggtaaaact cgcaggcggt taacataaaa agccacggat atatccgtgg ctttcgaata
     6481 ttttactgtg tgtattattc agttatttcg tactgaacag aatcaagcgt attataattg
     6541 cgagtcgata atcgacattt atttacgatt atttaccgac ctggtgacca ataacaccac
     6601 cgacagctgc accacctaat gtacccaacg tactgccatc ggtcagtact gcaccgccta
     6661 atgcccctgc acccgcgccg attgcggtgt tgcggtcccg tttagaccag ttagaacagg
     6721 cactcagaga cattgccaaa gtaattgcca gaacagccgc ggtcattttt ttgctcgtta
     6781 caaacataat actctctcct gaatttatga ttcacggaag taagctctct ataactataa
     6841 tagctaagaa taagcctggt gaaattattc cgtgaaatct gctcgcgcgc agggttatat
     6901 cacgcaggtg ataatgactt cctgttatat cgctgataat aattttatat cttaagcgtg
     6961 ttaataacag gtaaatagtc ttaattatca accaggaatc atcttagagc ggatgatttg
     7021 ccaaactgca aatcatccgt agagaaggga aatggttaaa tgtcaacgac gtgaacggtg
     7081 agtttactgt gctcaagatg catcagatcg ctggcgcgta ttctcgaatc ggtaatcacg
     7141 cgattgaatc gctcaacggg accgatggag tatgaatgta cagcaccaaa tttcgagctg
     7201 tcagtcagga ctatcgcttc gcactctttt tccagcacgg cattgaccac atcggtacgc
     7261 atcatatcgc gaccggtgaa tccagtttca ggttgccagc catcaatacc aataaatgct
     7321 ttgctgaaat gtacctgctg gatgcactgg cgtgtcaaag ggccaaccat actttcgctt
     7381 tttttctggt acacgccacc gagcaaaatg acttcacaag gcgcgtcttt tagcaaatgc
     7441 gcaatgtagc tgctgactgt gatgatagtg acatttttct tctgttcgcc cagagtccgg
     7501 gcgagcaggg cattactgct accattttcg ataaagatgg tttcgcccgg ttgaaccaga
     7561 gacgcggcaa actcggcgag ttcacgcttc agcgtatagt tgctcatcat acgggtttcg
     7621 acgtcatcac tatcaagcga aactgcaaag ccatgtgcac ggcggaggta actcagtttt
     7681 tcgagggtgt tgagatcctg gcgaatggta acttcagaaa ctccagtggc ttttgccaga
     7741 tcggttacgc taacctgacc ctggtcaatg accatctgta gaatagtttg ttgtcgggaa
     7801 ttcatcgctg taattcttat aacgttataa tactaaataa aaaaatactg acgcgccggg
     7861 catcctcaaa taggaaaacc tgaaggcacg attgttaaat ctcccacggt gccttcggga
     7921 tgtctggcgt agattcagca acctggcgca agagttctgc tttaagtatc tcgagattat
     7981 ggatcgcaga gtggtaatcg cctgccacaa ggatatctaa cacaatatca atacgttcgg
     8041 caacgcgctg ggcatcaaat tcagacatca aggacatcct tatagcaaaa gggggaaaat
     8101 gaataatgca aaaattgccg ctaaaagaga agtgtttaac agcaacggct aattatcatc
     8161 caagaatacg atatataatg acgggatata gcgctaagta tatatattca tctacttatg
     8221 cgcgcttcag atagcgttta taccagcgtt cgaaggcgac ggcgggcatc ggtttggcaa
     8281 acaaaaatcc ttgccgctca ttgatcccgt tcttggttaa aaaagcatct tcctttgcac
     8341 tctctacacc ttcggcgatc acctgaagat tcaatgcctg ggccacagcg acgatcgccc
     8401 ggaccagtga ctgcgagaca ggttgtttgt gaatatctcg aacaaaaacc tggtcaagtt
     8461 tgatggcatc gatcggaaag cgcgccagtt gcgaaagtga agagtagccg gtaccaaaat
     8521 catccagatg cacttgcgca cctagttggc taaattgttg aataacagac agtgccagtt
     8581 catcattctc aatcagacaa ctctctgtca gttcaacatc tatagggcag tattcaaaat
     8641 tgagttcctg gagaacctgt ttcagggcgg tgaaaatggt ttgatcggcg agctgacgtg
     8701 cagaaatatt taccgccact cgcaggttta tgcctttttc ccgccacttt gccacctggc
     8761 gtacgacatc gagaatcacc caacggccta aaggcacaat tagccctgac tcttcggcgt
     8821 aggaaatgaa gtccaacggt ggaatcaacc cacgttcagg tgactgccaa cgtactagtg
     8881 cttccagact gcgcacttcg ccacgctagg tgattttcgg ttgatagtga ataaccaact
     8941 gatcgttttc cagtgcttta cgcaagttgg tatccagcca gagatattca aatacccgtt
     9001 gattcatttc tggggtaaaa acgcaaaatt gtcctcgtcc gccttccttc gctgtgtaca
     9061 ttgctgtgtc ggcgtgacga ataatagccg tgctgtctga accatgttcg ggagagagtg
     9121 caatacctac tgaacagctg gtataaactt caattaaacc aatgcgaaag gggagccgta
     9181 agcgggtcaa aattcgtgat gccattgctt ccagcgcgct ttgtgaggtg ttggatgcca
     9241 gtaccagaaa ctcatcccca cctggacgcg ccaacacctg gtcatgttcg agacagctta
     9301 aaatagccaa tgacatgtcg cgtaataact ggtcaccaaa caaatgccca taggcgtcgt
     9361 tgaccttttt gaaattatcc aaatcaagat acacaacccc aactttattg ttatctgcat
     9421 gattaatagc gtgatcgatt aaatcctgca ttgcgttacg attcggcagt ccggtgatac
     9481 tgtcggtatt tgccagaata cgcagtcgct cctgagcgcg gcgctcttcg gtaatgtcgg
     9541 tgccggaaca gattaaaaaa atctcgtttt tgccactgcc gctgtggaca aatttattgc
     9601 gaaacagaaa cagccgctgg cctttacatg ttggtatcca cagttcgact tcatatgcat
     9661 tgccgcttcg aaaaaataca cggttattgc gcctggatgc cgcagcttca cgacggctca
     9721 taaacagttt aaacacgctt tgcccaatga cgtcgtgttc tttcaaccct gtgtaatctt
     9781 cacataaccg attgaagcgt tggatattcc cccggctgtc gagaatcact attgcggaat
     9841 gtgcttcaga aactacctgc tcagcaaagg aaagcccgtg tgataaatca cgagcaaccg
     9901 ccggtgtgtc atgccatgca gacgcgttgc cagcccattc ccgtttattg atttttcgtc
     9961 ctaccagatg cacagaaaga tcgctttcat cgacggtcag actcatcatc aggctggagg
    10021 tgatgaccgt catttccctg atgcgagcag
//
LOCUS       DAZGTR010000148         9670 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGTR010000148 DAZGTR010000000
VERSION     DAZGTR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.150"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..226
                     /locus_tag="ACHLCY_004193"
     CDS             <1..226
                     /locus_tag="ACHLCY_004193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371333.1"
                     /translation="QSSIKPVCPPLHHFSALNVADASLSHSDGSLLVCIIDRELRIKD
                     ITKHQNLIFRICRKGDVNKYLRMKTELVQM"
     gene            641..1807
                     /gene="dacD"
                     /locus_tag="ACHLCY_004194"
     CDS             641..1807
                     /gene="dacD"
                     /locus_tag="ACHLCY_004194"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP0371334.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAIDGQRRLIAVVMGADSAKGREEEAKKLLR
                     WGQQNFTTVQILHRRKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLN
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            1922..2395
                     /gene="sbmC"
                     /locus_tag="ACHLCY_004195"
     CDS             1922..2395
                     /gene="sbmC"
                     /locus_tag="ACHLCY_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP0371335.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            2593..3652
                     /gene="yeeA"
                     /locus_tag="ACHLCY_004196"
                     /pseudo
     CDS             2593..3652
                     /gene="yeeA"
                     /locus_tag="ACHLCY_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            3824..4153
                     /gene="tmaR"
                     /locus_tag="ACHLCY_004197"
     CDS             3824..4153
                     /gene="tmaR"
                     /locus_tag="ACHLCY_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP0371336.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(4254..4436)
                     /locus_tag="ACHLCY_004198"
     CDS             complement(4254..4436)
                     /locus_tag="ACHLCY_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP0371337.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            4490..4636
                     /locus_tag="ACHLCY_004199"
                     /pseudo
     CDS             4490..4636
                     /locus_tag="ACHLCY_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4925..5005)
                     /locus_tag="ACHLCY_004200"
                     /pseudo
     CDS             complement(4925..5005)
                     /locus_tag="ACHLCY_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5051..5245)
                     /gene="yeeW"
                     /locus_tag="ACHLCY_004201"
     CDS             complement(5051..5245)
                     /gene="yeeW"
                     /locus_tag="ACHLCY_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0371338.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            complement(5242..5615)
                     /gene="cbtA"
                     /locus_tag="ACHLCY_004202"
                     /pseudo
     CDS             complement(5242..5615)
                     /gene="cbtA"
                     /locus_tag="ACHLCY_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            complement(5704..6072)
                     /gene="cbeA"
                     /locus_tag="ACHLCY_004203"
     CDS             complement(5704..6072)
                     /gene="cbeA"
                     /locus_tag="ACHLCY_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP0371339.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            complement(6146..6367)
                     /gene="yeeT"
                     /locus_tag="ACHLCY_004204"
     CDS             complement(6146..6367)
                     /gene="yeeT"
                     /locus_tag="ACHLCY_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0371340.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6436..6912)
                     /gene="yeeS"
                     /locus_tag="ACHLCY_004205"
     CDS             complement(6436..6912)
                     /gene="yeeS"
                     /locus_tag="ACHLCY_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0371341.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(6928..7407)
                     /locus_tag="ACHLCY_004206"
     CDS             complement(6928..7407)
                     /locus_tag="ACHLCY_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0371342.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(7489..8310)
                     /locus_tag="ACHLCY_004207"
     CDS             complement(7489..8310)
                     /locus_tag="ACHLCY_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0371343.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPSITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(8531..8941)
                     /locus_tag="ACHLCY_004208"
     CDS             complement(8531..8941)
                     /locus_tag="ACHLCY_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371344.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVGVAQDKLKI"
     gene            complement(8957..9640)
                     /locus_tag="ACHLCY_004209"
     CDS             complement(8957..9640)
                     /locus_tag="ACHLCY_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371345.1"
                     /translation="MKLPVKLLMSLISLVRVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
BASE COUNT         2403 a         2290 c         2446 g         2531 t
ORIGIN      
        1 tcaatccagc atcaagccag tctgcccacc actgcatcat ttctctgcgc ttaatgtagc
       61 ggatgcctca ctatcacata gtgatggttc attactggtt tgcataatag atagagaact
      121 gaggataaaa gatatcacaa aacaccaaaa tctcatcttt aggatttgca gaaagggaga
      181 cgtaaacaaa tacctgcgca tgaaaacgga actagtccag atgtgatgtt tgtggtgatc
      241 acatacatca ttaacgatgc tcgttatggt gagtttgatg actacccgat gaagtgaaaa
      301 tagtgttgtg taccaaattg cgtaccaaac taaaatcaca aatcatgaaa cccttgttca
      361 tggcggttct caggggtatt gcgcgtaatc gtgaaacaaa aaggtagatt gttgcttacc
      421 gtcattcatc attaggttaa atccgttatt tctgctgtct gccagagtat caaatatcac
      481 cgtgctaatc agctttagcg cgacaatttg gcagcgagtg acaacagatc atgtcagata
      541 aaaatgagat agtagtcaca ttttcttgca ctttattcca gccagttcat aagtatttcc
      601 gtaaaaagaa cagctatttg aaacacctga gggtttgctg ttgaaacgcc gtcttattat
      661 tgctgcttct ttgttcgttt ttaatttatc gtctggtttt gcggcggaaa acattccttt
      721 ttcacctcag cctccagaga ttcatgccgg gtcctgggta ttgatggatt acaccaccgg
      781 tcagattctc accgcgggta atgagcatca acagcgtaat cccgccagcc tgacaaagct
      841 gatgacgggc tatgtcgtgg atcgcgctat cgatagtcat cgcattacgc cagacgatat
      901 tgtcaccgtg gggcgtgatg cgtgggcgaa agataatccg gtgtttgtcg gttcttcact
      961 gatgtttttg aaagagggcg atcgcgtatc ggtacgtgat ttaagccgag gtttaattgt
     1021 ggattccgga aatgacgcgt gtgttgctct ggctgactat attgccggtg ggcaacggca
     1081 gtttgttgaa atgatgaaca actatgccga gaagctgcat ctcaaggata cgcattttga
     1141 aacagtgcat ggtctggatg cacctggcca gcatagctcg gcttatgatt tagccgtgct
     1201 ttctcgcgct atcatccacg gcgagcccga gttttatcat atgtacagtg agaaaagcct
     1261 cacctggaac ggtatcaccc agcaaaaccg taacgggtta ttgtgggata agaccatgaa
     1321 tgttgacggc ctgaaaacgg gccatacttc tggtgccgga tttaatctta ttgcttcggc
     1381 tatagacggg cagcgtcgcc tcattgcagt ggtaatgggt gctgacagcg caaaaggtcg
     1441 tgaggaagag gcaaaaaaat tactgcgttg ggggcaacaa aactttacta cggtgcaaat
     1501 tttgcaccgt aggaaaaagg tcggtacgga acgcatctgg tatggtgata aagaaaatat
     1561 cgccctggga acggaacaag agttctggat ggtgctaccg aaagccgaaa ttccacatat
     1621 caaagccaaa tataccctta atggtaaaga gctcaccgcg ccaattagcg cccatcagcg
     1681 ggtaggggaa attgaacttt acgaccgtga taaacaggtg gcgcactggc cgctggttac
     1741 cctggaatct gtcggggaag gcagcatgtt ttctcgcctg agtgattatt tccaccataa
     1801 ggcctgacct ttcttttgca gcagactgga gtgcgagtct gctcgcataa tcaacactca
     1861 ttccttgtgg ttttaatatt gcaactatac tgtatataaa aacagtaata atggaggcgt
     1921 catgaactac gagattaagc aggaagataa acgtaccgtt gcaggtttcc atctcgttgg
     1981 cccgtgggaa cagacggtaa agaaaggttt tgagcagttg atgatgtggg tagatagcaa
     2041 aaatattgtg ccgaaggagt gggttgctgt ctattacgac aatccggatg aaacacccgc
     2101 cgaaaaatta cgctgcgaca ccgtcgtgac ggtaccgaat aactttacgc tccccgaaaa
     2161 cagtgagggc gtcattctga cagaaatttc aggtggtcaa tatgcggtgg cggtggctcg
     2221 tgtagtcggt gatgattttg ctaaaccctg gtatcagttc tttaatagcc tcttgcagga
     2281 cagtgcttat gaaatgttac caaagccctg cttcgaggtt tacttgaaca atggcgcgga
     2341 agatgggtac tgggatatcg aaatgtatgt tgcggtgcag ccaaaacatc actaattcat
     2401 ctcagggcgg tgtgttgacg cgaagaccac tctttttttg aaagcgaaaa gagtaagatg
     2461 cgcctttcaa ttttttcgct cctgccggga aattacactg ttcccggttt gtccgtcgga
     2521 taattcagag gcgcgccttc tggccgacag atgagttatg agcgctttta atcttcatta
     2581 cggagtttct gcgtgcgtgc cgataagtca ttaagcccgt ttgaaatccg ggtataccgc
     2641 cattaccgca ttgtgcatgg tactcgggtc gcgctggcat tcctgctcac ttttctcatt
     2701 atccgcctgt ttactatccc ggaaagcacc tggccgctgg tcaccatggt ggtgattatg
     2761 gggccaatct cgttctgggg gaacgttgtc cctcgcgcat tcgaacgtat tggcggtacg
     2821 gtgttggggt cgattttagg tcttatcgct ctgcaactgg agttaatctc gttaccgctg
     2881 atgttagtct ggtgcgcggc ggctatgttt ctttgcggtt ggctggcgct gggcaagaaa
     2941 ccgtatcaag gtttattgat tggggtgacg ctggcaattg ttgttggttc cccgacaggt
     3001 gaaattaata cggcgttatg gcgaagcggc gatgtgatcc tcggctcttt actggcaatg
     3061 ttgtttaccg gtatctggcc acaacgggcg ttcatccact ggcgcattca actggcaaaa
     3121 agtctgaccg agtataatcg gtatctatca atctgcattc tcaccgaact tactcgaacg
     3181 cccgcgtctg gaaagccatc tacaaaaact cctgaccgat gccgtgaaaa tgcgtgggct
     3241 gattgcgccc gccagcaaag aaacccgtat tccaaaatcg atatatgaag gtatccagac
     3301 cattaatcgc aatctggttt gtatgctgga gttgcaaatc aatgcatact gggccacgcg
     3361 ccccagccat ttcgtgttat tgaacgcgca aaaacttcgt gatacccagc atatgatgca
     3421 gcaaatactg ctgagccttg ttcatgcgct gtacgaaggt aatccgcagc cggtttttgc
     3481 caatacggaa aaattgaacg atgctgtgga agagctgcgt cagttgctga ataaccacca
     3541 tgacctgaag gttgtggaaa caccaatcta tggttatgtg tggctgaaca tggaaacggc
     3601 acatcagctt gagttgctat cgaatctgat ttgccgggcc ttgcgcaaat aattcctgaa
     3661 cttcagaatc atcttgctgc tgcttcgatt cagcaaggat aaagggtatg atagtgaaaa
     3721 gggataaaag cattgtcatc tgcggcagct atgagtaatg ttggccctaa cgaatagcgg
     3781 ttgcttaaac gaatccgact ctcacatgat caggggtata aaaatggaaa ctaccaagcc
     3841 ttcattccag gacgtactgg aatttgttcg tctgttccgt cgtaagaaca aactgcaacg
     3901 tgaaattcag gacgttgaga aaaagatccg tgacaaccag aagcgcgtcc tgttgctgga
     3961 caacctgagc gattacatca agccagggat gagcgttgaa gcaatccagg gcatcatcgc
     4021 cagcatgaaa ggtgactatg aagatcgcgt tgacgattac atcatcaaaa atgccgagct
     4081 ctccaaagaa cgccgcgata tctccaaaaa gctgaaagct atgggcgaaa tgaaaaacgg
     4141 cgaagcgaag taattcccgt tttattcaat gagggttgcc cggcaaccct cattgctcat
     4201 tgattcttat ctgtgtatca ccgtcatcat tctcatctga gaaccaatcg aaattaacaa
     4261 cagccttctt ctgtatgcag caaggcaaaa agttctgtaa ctccattgtt attaactgca
     4321 ctggttacta acacgttgtg cgctccagct tcccgtaacc aacttttcac caaagatatt
     4381 tgttccatgc tggctaaatc tgctttggtt actactccaa tgaccgggtg attcatggcc
     4441 cggtagggcg tttttacctc taattcctgc ttcagcgctg agattcctgc ttgttgccgg
     4501 aaaagcaggc acctgccgtc tgaacggtat cgatccggaa gcgtatctgc gccatattct
     4561 gagcatactg ccggaatggc cctccaaccg tgttgacgaa ctcctgccat ggaacgtagt
     4621 tctcaccaat aaataagcgt caatacggtg ctccgttgac gcttacctta atccgttttc
     4681 tgagcttaga tttttctgtt cctacccaga ccgtggattt ggaattcatt ttttgtcagg
     4741 tcaatgctaa tcgttaaaac ggccataacg cacctccact ggaaggttta tcagtaatat
     4801 cctaccgttg cttcatggtc ggggcgtcca ttccattatt atcggttttc ctttctgttt
     4861 ataaatgaaa acgccagctg tattcaggct ggcgtcaggg gaaatgaagc ctgttgagtg
     4921 agattcaccg gttctggtgc agaagctgca gatggcgcag ggtaaggaca tcttctcttg
     4981 cctgcttaat acaggacaac agtgctgttt ctgtggttaa tggcactata ccctgcatca
     5041 ctgcattgtc ttacagcaac tggtgagggc tggcataagt tgccggtgtt tcggtagagt
     5101 cacggtcatc caccacgcgg agtgaataac ctttttcagc ggccttatta atcagttcag
     5161 tgatattaac accatcaatg tcaacgacaa tgtgccccat atccagcgcc tgtacgttaa
     5221 cgctgtcggc ttccagtgtc atcatttcgc ctccggatac ttacccaggg taatgttatt
     5281 taccgttctg taattgtcgc gggtcatcag gccggtcgcc ctgcgagccc ggaggatatc
     5341 gatgctgttt attaactgag agcgggtaca ggcgctgaat cccggctggt cggtagcacc
     5401 agcgcgtatt tttccacgag aaagttcacc gcatcacaca gtgaaatgcc tgcctcaata
     5461 tgctgctcaa tcacacgttc atcggcaaaa ggtgtgtcat tcagtgtgag gccatagtgc
     5521 tggtccagca gtcgggacag cagtatctgc cagatttcaa caggagacgg gcgagaactg
     5581 gtcgcctgcc cgggtaatac aggtaatgtt ttcatactga agattttcct gatatgcaga
     5641 tataaaaatg gaaaagtggc gtggtgaaaa caccagaccg tagcagaagg ctattctgga
     5701 gagttaattt ttcatttcgg gcgtcggata aacagccaga taaacgtaac cacaactgct
     5761 gagggtatcg gctttgcagg tcagcccttt tgcatacagc gtgacggtat gctgatggcg
     5821 gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca gcggaaaggc
     5881 ctggtccagg tggtacgcat ctgcatcgct gaacaggcct ctaataccgg cgcggtcggc
     5941 aaggtaatgc aaccggttac cctcctgcac cagacgtgcc ccgaaacagg gcgtcacggt
     6001 gcagggcagc ccccaccagg ggcggtcgtg attgtcgtcg ggaagtgttg tcccggggag
     6061 tgtgtctgac acgataaaat ccttacagaa aatcggctaa gaatggtccg gtagtggcga
     6121 taattctgct catcagaatt cccactcagt tcagggtgac gctcatcagc cggacatacg
     6181 gaccaaaact gtccttacgg cgttctgcaa acacggccag cacaccggga atatcctgca
     6241 cttcacgacc ggtatacgct tcagcactgc cgtgccagcg gtatttgccg gtacagaacg
     6301 gaaaaagacg ggatgccgga tgctgacggt gaatacgcat ggcttcgcca cgggtgatga
     6361 ttttcatagt gggatacctc tgaagacaga agataaaagt gaaaacaggt gtgatgtggt
     6421 tgtaacggtg acgggttaaa gcagaccgtg ttccgcaaag gagaaaacct ggttgccacc
     6481 aactatcaga tggtccggta cccggatatc caccaggccc agtgcctgta ccagacgttc
     6541 cgtgataagg cggtctgcct tgctgggtgt gacttcaccg gacgggtgat tgtgtgccag
     6601 caccacagca gcggcattgt gatacagggc gcgtttaatc acttcccggg gatggacttc
     6661 cgtgcggttg atggtgccgg tgaagagggt ttcaccggca atcagctgat tctggttgtt
     6721 cagatacagc acccggaatt cttcacgctc cagtcccgcc atgttcagaa tcagccattc
     6781 ccgtgctgca cgggtggagg tgaaggccac gccgggttca tgaagatggc ggtccagggt
     6841 tttcagggcc cgttgaatga ggctgcgctc gccgggcgtc atctctccgg gcagaaagga
     6901 aagttgttgc attgtgcttc tctccattca gtcgatgatg cgcataatgg cgctgcattc
     6961 cggatgctgc agggcgtaat cccgcaaccg gtaataatgg accgtcatgg cataattctc
     7021 cgtacgacag gcatgatgac tgtacgtcat cagacaggcg gcaataccgg cggcttccgg
     7081 gctcatttct gcgcggttac cgttcatggc attgaacagt acccatgttt cgtcatcatc
     7141 gtcatccggt tcgggtgcca taaatgcccc gccgttgttc agggtgtaca gattccagat
     7201 accaccgcag tagtcttcgc acagacggtc catccagccg aagacacggg gctccagggt
     7261 cacccactgt ggaatgaggc caaaatgctg cggccagaag ctgatgcgct gttcatcagg
     7321 gacgaggctg gcaaccaact gaggcggatt attctctggt gttgtagctg aataaattgt
     7381 gggggtattc tgagaaacgg ttttcatgag ataactcctt aaataacaaa taaatgtgtg
     7441 tttttcagga acgaaagctg aatgattccc tcactcaggg aaacggcatc agcgcaggct
     7501 ctccagcatc gtttctgcca tcacccacaa tgcgcgattg agcttaatgt cggtatcaat
     7561 gctgtgaatg gcccgggtat ggatacgttt tccttttgca ctgcgaccgg aaatcccgcc
     7621 tttcagcata ttctcctgga tggtctgata tgcgctccac aggtccttac cgtaatcctc
     7681 ccggcgtcgc ggcgtcagaa tgtcggcggt ggtgacgggc tgatgttcgt caccataacg
     7741 gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga ccagcgactg
     7801 catggcatca cgcttctcct ctatccggtc aaacacgccc accacctcgt aagccccttc
     7861 gataactttc tccactacat ttccccggtg tggaacacgc acttccccca gagactggcc
     7921 acagacgcac ccgttctggc agacgaacct gaagtaaccc ggcagcatct ggtagctgga
     7981 ggtaccgtca tgagagttga gcagaataat ttcagggaca tgttctccgt ttatctctcc
     8041 ggcccgccgc agacgcagca tgtgttttgt gtatccccgg cggcccgggt cgcgcacacg
     8101 ggtctggcag gcgaagaacg gctgaaagcc ttcccgctgc agattttcca ggacggtaat
     8161 ggagggaatg tacgcatacc gttcactgcg ggaggtgtgc cggtcttccc caaaaatact
     8221 gggtacatgg cgtatcagtt cttcatgtgt cagcggacgg tcacggcgta tctggtttgc
     8281 agcataacca aaacgactgg ctaatcgcat actttgctcc ttatcggtgg ttaagattta
     8341 ctggtgcaat aaatgaaaaa agccgtgtct cccggagaag acgcggcctg acgggtgaaa
     8401 tgaataaggt ttattgtctg agaggccttt cactggcgag ccacgcatta agcggctttc
     8461 cggtaacaat ggctggggct gtatttacct ttgatattaa taatgtgcag agaaacgcat
     8521 ggtttactcc ttagatttta agtttgtcct gggctacacc gacaggtttt ccggatgtgt
     8581 cgatgactcc taccgtcggt acgccatttt tgatttcgaa gaacggtttg ttgctacctg
     8641 agcacagttg attgtccccg tattcaatca tttgtgcttt cccggttttc aggttaacta
     8701 ccgagacagt gtagtcgtag caccaggcac caccacgttc aagcacgaaa acatagctgt
     8761 tatctttata gttgaactga ttctttgtct ggcaattcat accgtcaatt tcggtacagg
     8821 aaaacgcttc tgtagtatta acgtatttga tgttatttgg cagttttact tgtttcgcag
     8881 tagcgaagac agagaacaga atagttacag caacgagtaa agttttgata aaagttttca
     8941 tgatagactc cttaaattag ttaaccgcag ctacgtagtt tttcaccaga tacgcgaatt
     9001 catgcacgcc aatttcaccg aaccaaacca ggtaggccgg gtcatctaaa ccaaagtaac
     9061 ggtagccaac aacttcatgg gtttttgggt tatagagtgt tacgataaag tcccagccgc
     9121 aggtattggc tttgcaaccg tcgccctgga accagactgt accgttaatg tttactggcg
     9181 tgatacctcc accagggcca tccatatagc cgcctttacc gaacatgccg ctcagtccga
     9241 ccggttttaa tgctttgtcg atagcggctt tgtaggtagc gtcaccttcg tacaactcgt
     9301 acgttgatgg acacgctcca ggttgttcag cccaggcttc cgggttacaa ctaagagctt
     9361 gttttacggt agccgcttct gcgttaaagt taaagcccgt gaacagaacc gccagaaata
     9421 acataagggt atgtaaacgt ttcattgtaa atattcttcc gttgtaaatg gcgctggaaa
     9481 atttctcatc agtgaaacct tccggaagtt tccccccctt gtatgatgta tatcgccagt
     9541 attttatttc atcccggctg taatttttat attccccggc acgtgcaata acgcggacca
     9601 gagatataag gctcattaat aactttacag gtaatttcat aattaaattt ccagtgagtg
     9661 aattgacagg
//
LOCUS       DAZGTR010000149         9505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGTR010000149 DAZGTR010000000
VERSION     DAZGTR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.151"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..490
                     /locus_tag="ACHLCY_004210"
     CDS             <1..490
                     /locus_tag="ACHLCY_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0371346.1"
                     /translation="YAQSKVQDFKNIKGVNLQYRYEWDSPVSVVGSFSYMKGDWADSH
                     RDEADDFYRHQADIKYYSFLAGPAYRLNDYISFYGLVGISHTKAKGDYEWRNSVGADE
                     SDGYLSESVSKKSTDFAYAAGVIINPWGNMSVNVGYEGTKADIYGKHSVNGFTVGVGY
                     RF"
     gene            626..1417
                     /locus_tag="ACHLCY_004211"
                     /pseudo
     CDS             626..1417
                     /locus_tag="ACHLCY_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308008.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1422..2792)
                     /locus_tag="ACHLCY_004212"
                     /pseudo
     CDS             complement(1422..2792)
                     /locus_tag="ACHLCY_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2812..3159)
                     /gene="tnpB"
                     /locus_tag="ACHLCY_004213"
     CDS             complement(2812..3159)
                     /gene="tnpB"
                     /locus_tag="ACHLCY_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0371347.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDAPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3156..3830)
                     /gene="tnpA"
                     /locus_tag="ACHLCY_004214"
                     /pseudo
     CDS             complement(3156..3830)
                     /gene="tnpA"
                     /locus_tag="ACHLCY_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
     gene            4084..4704
                     /locus_tag="ACHLCY_004215"
                     /pseudo
     CDS             4084..4704
                     /locus_tag="ACHLCY_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
     gene            4822..5034
                     /locus_tag="ACHLCY_004216"
     CDS             4822..5034
                     /locus_tag="ACHLCY_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP0371348.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEYCYRRCRGRV"
     gene            5279..5740
                     /locus_tag="ACHLCY_004217"
     CDS             5279..5740
                     /locus_tag="ACHLCY_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0371349.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            5786..5995
                     /gene="hha"
                     /locus_tag="ACHLCY_004218"
     CDS             5786..5995
                     /gene="hha"
                     /locus_tag="ACHLCY_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP0371350.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLERIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            6033..6620
                     /locus_tag="ACHLCY_004219"
     CDS             6033..6620
                     /locus_tag="ACHLCY_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFP0371351.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWRLFRMSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRLA
                     GIPLLRSHDARKLLQMTGEWLNTTGAAQQSPEHRS"
     gene            6860..7117
                     /locus_tag="ACHLCY_004220"
     CDS             6860..7117
                     /locus_tag="ACHLCY_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0371352.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            7352..7426
                     /gene="tap"
                     /locus_tag="ACHLCY_004221"
     CDS             7352..7426
                     /gene="tap"
                     /locus_tag="ACHLCY_004221"
                     /inference="COORDINATES: protein motif:HMM:TIGR03475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP0371353.1"
                     /translation="MPGKVQDFFLCSLLLCNISAGRCD"
     gene            7419..8276
                     /gene="repA"
                     /locus_tag="ACHLCY_004222"
     CDS             7419..8276
                     /gene="repA"
                     /locus_tag="ACHLCY_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0371354.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFTANGETVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            8979..9239
                     /locus_tag="ACHLCY_004223"
     CDS             8979..9239
                     /locus_tag="ACHLCY_004223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371355.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRRKRTRVARYLFSLNYNSMTFGRHPDSCKKKHGLAG"
     gene            9252..9491
                     /locus_tag="ACHLCY_004224"
     CDS             9252..9491
                     /locus_tag="ACHLCY_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFP0371356.1"
                     /translation="MKTTNSILCVIASVIAAGQNQISITELVSLSGISRQSVKRAIQI
                     LEQAGQITVTRTTTNGKRGANTYYLPDQDLGGGCR"
BASE COUNT         2350 a         2298 c         2549 g         2308 t
ORIGIN      
        1 ttatgctcag agtaaggtac aggactttaa gaatattaaa ggagtgaatc tgcaatatcg
       61 atatgagtgg gattctccgg tcagtgtcgt cgggagtttt tcgtatatga aaggtgattg
      121 ggcggacagt catcgcgatg aagctgacga tttttacaga catcaggctg atattaaata
      181 ttattcattt ctcgcaggcc cagcttatcg tctgaatgac tatatcagtt tttatggttt
      241 ggtggggata tctcatacta aagcgaaggg agattatgag tggagaaaca gtgtgggtgc
      301 tgatgagtct gatggatatt taagtgagtc tgtatcaaaa aaatccactg attttgctta
      361 tgcagctggt gttattatta atccgtgggg caatatgtct gtaaatgttg ggtatgaggg
      421 aactaaagca gatatatatg gaaaacattc ggtcaatgga tttactgttg gagttggtta
      481 tcgtttttaa tcattagacg ggactggata attgcacttg cgtagtaaag tttactaaat
      541 gtttgtattt taatgaagta ttcgacatat tcccttcaga cggaatatgt cgacggttat
      601 ggttttactg gagcatgagt atgttatggc gaaactgata aatatatcag cgtccactga
      661 ggagagaatg acgccagggg agaggagggt ggcttcacga ctggagagct ttctgaatga
      721 tgattgcttt gtctggtacg atatcccggt gggacggaaa aaccgacatc cggattttgt
      781 cattattgac ccggataacg ggctggtatt tctggaggtc aaggactgga ctgtatccac
      841 attacgtaaa gcaaatcagg agcaggttac actggaaacc gacggtcttc tgaaatcaga
      901 aataaatccg ctggtacagg ttcgtcgtta tgcctgtgat acggttaatg ccttacctgc
      961 tgacccgtgc ttacggcaga atgacggtca gtataagggg cggctgaacc tggcctgggc
     1021 gtatggtgtg gtgtttaccc gcattacccg tcagcaactg aaggcactga cagggaataa
     1081 tgagaatgcg gtggaaaaga tttttccttc agcacagaca atctgtcagg atgaaatgac
     1141 gcagtctgtt ttacctgagg tattccggca aaaaattgcc gggatgttta ccacggggtt
     1201 cagaacccgg gtaacacccc ggatgaggga tattctgcgc gcccatctgt ttcctgaagt
     1261 gaccgtgaaa cagaactcac aaatcaaaat tatggatatt cagcaggaga ttctggccag
     1321 aaatattggt gacgggcatc gtgtcattca tggtgtggcc ggttccggaa aaaccatgat
     1381 tcttttgttc cgttgccttt acctggcaga aaccacgtcc ggtagttacg ccgccccagt
     1441 gcaactaccc ggagggcgtt ttcacacagg ttattgtcga tctccaccca gccattgcgg
     1501 cagtattcgt tcagagcgtc ccattgcttc agcagatagg tgaacgcttt cgctgtatcc
     1561 gagtggcgcg acagcgtgtt catctgcccc tggagccact catacaacga ctgcattagc
     1621 ggtaccgttc tggcttttct gaccgccagt cgctcttctg ccggactgcc gcggatctca
     1681 gcctcgatag cgtacagttc cccgatacga cggagcgctt ctcccgttac tgtcgttgga
     1741 tggcggacat gtacatcgtg gatcttgcgt cgggcatgtg ccatacaagc cgcttcggtt
     1801 gcctgaccgc tttcgtaaag agcattgtaa cccgcatatg catcggcctg caggatacct
     1861 ctgtagtccg ccagatgttg ctgtgggtgg atgcctttgc ggtcgggaga gtatgagaac
     1921 cacaccgctg ccggcatggt cgaaccggcg ttgcgatcgt cacgaacata gacccacagc
     1981 cgtccggtac gggttttacc ctgacccggc tccagtacat ttaccggcgt gtcatcggca
     2041 tgcactttac ccggcatcag cacatagtgc ttcagttcat catacagcgg gcgaagctgc
     2101 tctcccatga tgtcaaccca gcgccccatc gtattgcggt gcagctccac gccctggcgg
     2161 gcatagattt ccgactgacg gtacagcggc agatgctcgg cgaacttagc catgattatg
     2221 cgggccagca gagccggact ggcgtaactg cgctcgatgg gttttggtgg ctgcggagcc
     2281 tgaactatac agtcgcaccg gctgcaggcc cgttttgggc gaaccgtttc gattaccctg
     2341 aacgcggtgt tgatgatatc cagttgttca gagatgcttt ctcccagcgg tttcagtttg
     2401 ccgctgcaga cggggcattc ggtttctgcc ggggagataa cctgcctgtc acggggaagt
     2461 gttgccggaa gtgctttgcg gacgggagag tctgatgttt tcggcgctgt ctctccggcc
     2521 attgaggtga gttgcaactg cgcctcacca agcctgttct ggagctcggt tatacgcgtt
     2581 tctgcccgtg cgatcttctt ttctatcttc tcgcggcttt tctcgctgct gcgaccgaac
     2641 aacattctct gcagtttagc gaccagcgct ctgagtgagc tgatctcgcg ggcatagccg
     2701 gttatttcac cagacagacg gacgataaca gcctgctgtg cgatcagcag ggccttcagt
     2761 tgctcgatgt cgtcggggag tgtgttgttc attcccctgt tttatcacgg gttatatccg
     2821 gatgccaggc cgttctgtcc gtttgggatg ttgccatgcg atcccctcca gtagcatgga
     2881 taactgagct ggcgtcaggt gcactttccc ttcccgggtt accggccaga cgaagcggcc
     2941 ccgttccagg cgtttggcga acaggcataa cccgtcacga tcggcccaca gtattttcac
     3001 cattttgcca ctgcggcccc ggaagacgaa gatatgcccg gagaacgggg catctttcag
     3061 cgtgttctgc accttcgaag ccaggccgtt gaagccacaa cgcatatctg tgatgccagc
     3121 gatgatccag attctggtac cggttggcag cgttatcatc gggtacctcc tttcatttcg
     3181 cggattagcg cccgtaacat ttccggagtg agagggtcaa acagttttac cacacctgat
     3241 ttaagatgca gctcgcaccg tgggacgttt ccgggatcac tctcagggca ctcatcaggc
     3301 ttgttacgcc agaagggatt tgtaactggt ctggtcggct ctggcgtatc agtcagagcc
     3361 accgggacag gcatgcattc ctgtatgtca tcatcgctca gtaagccgtc ctcgtactgg
     3421 cttttccatt taaacagcag gttatcattg ataccgtgct ctctggcgat ccgggcaaca
     3481 acagcaccgg gctgtaatgc ctacttagcc agacggacct taaattcacg gctgtagctg
     3541 gctcgccgtt cttttcgcca tgtgccttcg ctgatttgag gctctgttaa ttccttcttt
     3601 ctgttggcat aaaggatggc gtcaagctga gctaatgaaa ctgaatcggg caatggccat
     3661 gcgataccgg atgcaataaa tcgctgaaaa agcgtatgta ttgtggaatg actgagacct
     3721 agacgctgag cgatggcccg gatggtcagt ttatcttcaa atcttaaacg cagagcatca
     3781 ggcaaataag aacggaagca gggaatatct ttttttgtct gggaattcat cgttcgtgtc
     3841 catctatata gatgggcgcg attgttgcca gacaggacaa ttttcacaag acgtcgcaga
     3901 tggggcgctt acacaacaat ggcgcgaggg aaagctgcta ttaccttctt cagagagccc
     3961 ccagctactt cctgtgactc tcgatgcagc tgccgaacag ccagaatcgc tcgcagagga
     4021 cccggaaacc ctcagtatca gctgttgtca acgacggatg actattgcac cgttcgtgac
     4081 agtgtgctgc aagcggatgc gtacgccggg ttcaacgagc tgtatcgcaa tggtgggata
     4141 acggaagctg cctgctgggc tcatgcccgc cgaaagatcc acgatgtgca cgtccgcatc
     4201 ccgtcagcac tgacggaaga agccctggag cagatcggtc agttgtacgc catagaggcg
     4261 gatataaggg gaatgccggc agagcagcgg cttgctgaac gtcagcgaaa aacgaaaccg
     4321 ctgttgaaat ccctggaaag ctggttgcgt gaaaagatga agaccctgtc gcgatactca
     4381 gagttggcga aggcgttcgc gtacgcactt aaccagtggc cggcactgac gtactatgcg
     4441 aacgatggct gggtggaaat cgacaacaac atcgctgaaa atgccctgcg ggcggtcagt
     4501 ctgggtcgta aaaacttcct gttcttcggc tctgaccatg gtggtgagcg gggagcgcta
     4561 ctgtacagcc tgatcgggac gtgcaaactg aatgacgtgg atccagaaag ctaccttcgc
     4621 catgtgcttg gcgtcatagc agactggccg gtcaaccggg tcagcgaact gcttccgtgg
     4681 cgcatagcac tgccagctga ataacacatc cccgtcaata cggccctcgc tgtacgctta
     4741 caaccttctt ccatacccgg cagaagaagg ttataaaaat ttataacaca ttcaccggcc
     4801 tggattaaag ggggaaggaa aatgaacgga acagggcgtg attcgcaaaa ctccggggta
     4861 tggcgctacc ggcaggtcgg acgccgggct gtccggctgg aagtcagggg ggactggccg
     4921 ggagccgctg aggcatggct acgggctgcg cagtctgctc cccggacaga ctggcagcag
     4981 tttgcccgaa aaagggctga atactgctac cggcgctgcc ggggaagggt gtaacgaaaa
     5041 agcagccttc attactccag ggattcagaa ggctgaataa tgcatgagat ttgctttttt
     5101 taatatccgg tcgggcagta tgatcggaca gttctgagta gcacagagtt ttttccgtat
     5161 taagtcgtta ttttatattt tgtgattaat ttcacaaaat aaggtgttat tcagtgtgtg
     5221 ctgcgatatt caggattacc tgaaatacag gtgccattta tctgatatgg agaataacat
     5281 gaggaaatat attccactgg tattatttat cttttcatgg ccggtattgt gtgcagatat
     5341 tcatggacgg gttgttcgtg tactggacgg tgataccatc gaggtcatgg actcacggaa
     5401 ggcagtgcgg atcaggctga taaatattga tgcaccggaa aagaaacagg attatggccg
     5461 gtggtcaacg gacatgatga aatcactggt tgcaggaaaa acggttaccg tcacatattt
     5521 tcagcgagat cgttatggtc gtattctcgg gcaggtttat gcaccagacg gaatgaatgt
     5581 taatcagttt atggtccggg ccggggctgc ctgggtatat gagcagtaca acacagatcc
     5641 cgtgctgcct gtccttcaga acgaagccag acaacaaaaa cgtgggctct ggtcagatgc
     5701 tgatccggtt cccccctgga tatggagaca ccgtaaataa agcgatggca atcttatcat
     5761 tcatctgttc tcagcgggaa taaaaatggc gaaaacaaaa caggaatggc tatatcagtt
     5821 acggcgatgt tcatcagtca atacgctgga aagaattatt cataaaaaca gagacagttt
     5881 attaaacagt gaacgtgaat catttaattc agcagcagac caccggttag cggaattaat
     5941 caccggaaag ttatatgaca ggataccaaa agagatatgg aaatatgtaa ggtaataatt
     6001 cagcataatt aattaccagg agcagataaa caatgaagct gattattttt attctcattg
     6061 ttctgattat tgcagcgtta cttatccgga ttatcctcag aagtgtaaat cagcattctc
     6121 ctttgctgat gcaattgcat gcggcaggta tacggaccgg tgatgcagag cgaatactgt
     6181 ccggcggtga atactggcaa cgtcagaaga cgctgctgac agaaagggaa gtcagtttta
     6241 tgaaaggact gttcagaatt gtggatatga agcggtggta tctgtgtccg caggtacggg
     6301 tcgcggatat cgtccagctg aacgggaata tccggccacg atcacgccag tggtggcggt
     6361 tattcaggat gtctcagtgg catgttgatg tggttatcgt tgagcggcgt tcgttcagta
     6421 ttgttgcagc agtagagctg gatgatgcca gccatttacg accggaacgc agacgccggg
     6481 atattcttct ggaagaggtt ctgaggctgg ctggtattcc gttgctcaga agccacgatg
     6541 ccagaaaact gctgcagatg accggagaat ggctgaatac aacaggggct gctcagcagt
     6601 caccggaaca tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc ggaaacggga
     6661 aaaagcaagt tttccccgct cccggcgttt ccataactga aaaccatact atttcacagt
     6721 ttaaatcaca ttaaacgaca gtaattcccg ttgatttgtg cgccaacaca gatcttcgtc
     6781 acaattctca agtcgctgat tttaaaaaac tgtagtatcc tctgcgaaac gatccctgtt
     6841 tgagtattgg ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct catctggcgc
     6901 aaaacgagca tacagaaagg gtaaaccttt gagtgaagca gaacgacaga gagcagcttc
     6961 tgcgcgcaaa cgatctgtat gtaaagagat caaggttttc gtcagaccag aactgaaaaa
     7021 ctaccttacc agtctgtgtg cagaggaagg ggtaactcag gcagagttga tagaaaggct
     7081 tatcgagaaa gaggcctgtc acaggaacat gatgtgatgg gatcacattc ttgctagttt
     7141 tttctgtcag tgctagatta ctgatcgttt aaggaatttt gtggctggcc acgccgtaag
     7201 gtggcaggga actggttctg atgtggattt acaggagcca gaaaagtgaa aaccccgata
     7261 atcttcagca agtttggcga ctaagagaag attaacgggg ctaacaagaa accgcataga
     7321 agctgttgct ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     7381 gtgctcactc cttctgtgca acataagtgc aggaaggtgt gactgatctt caacaaacgt
     7441 attaccgcca ggtaaagaac ccgaatccgg tgtttacacc ccgtaaaggt gccggaacgc
     7501 tgaagttctg cgaaaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     7561 ctattcatgt ggcgcatgcc cgttcgcgtg gtctgcgtcg gcgcatgcca ccggtgctgc
     7621 gtcgacgggc tattgatgcg ctgctgcagg ggctgtgttt ccactatgac ccgctggcca
     7681 accgcgtcca gtgctccatc accactctgg ccattgagtg cggactggcg acggagtctg
     7741 ctgccggaaa actctccatc acccgtgcca cccgggccct gacgttcctg tcagagctgg
     7801 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgaca
     7861 tcacgttcac accggctctg tttgccgccc ttgatgtgtc tgaggatgca gtggcagctg
     7921 cgcgccgcag tcgtgttgaa tgggaaaaca gacagcgcaa aaagcaggga ctggataccc
     7981 tgggtatgga tgaactgata gcgaaagcct ggcgttttgt gcgtgagcgt ttccgcagtt
     8041 accagacaga gcttaagtcc cgtggaataa agcgtgcccg tgcgcgtcgt gatgcgaaca
     8101 gggaacgtca ggatatcgtc accctggtga aacggcagct gacgcgtgaa atctcggaag
     8161 ggcgctttac tgctaatggt gagacggtaa aacgcgaagt ggagcgtcgt gtgaaggagc
     8221 gcatgattct gtcacgtaac cgcaattaca gccggctggc cacagcttcc ccctgaacct
     8281 gacctcctct gaataatccg gcctgcgccg gaggcttccg cacgtctgaa gcccgacagc
     8341 gcacaaaaaa tcagcaccac atacaaaaga caacctcatc atccagcttc tggtgcatcc
     8401 ggccccccct gttttcgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     8461 ttaaactgca agggacttcc ccataaggtt acaaccgttc acctcataaa gcgccagccg
     8521 ccagcgttac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     8581 taattacatt catttaaaaa gaaaacctat tcactgcctg tcctgtggac agacagatat
     8641 gcacctccca ccgcaagcgg cgggccccga ccggagccac tttagttaca acacacaaag
     8701 acaaccacca gaaaaacccc tgtcctgcgc agaactgaaa ccacaaagcc cctccctcat
     8761 aactgaaaag cggccccgcc ccggcccgaa gggccggaac agagtcgctt ttaattatga
     8821 atgttgtaac taataatcct catcgctgtc agtcttctgg ctggaagtcc tcagtacacg
     8881 ctcgtaagcg gccctgacgg cccgctaacg cggagatacg ccccgacttc gggtaaaccc
     8941 tcgtcgggac cactccgacc gcgcacagag gctccatcat ggctgaaagc ggatatggtc
     9001 tggcagggct ggggatgggt aaggtgaaat ctatcaatca gtaccggctt acgccgggct
     9061 tcggcggttt tactccggta tcacatgtaa ccacagcgtg ccgccttcca tgccgctggc
     9121 gcggcataag gagaaaaaga acgcgcgttg ctcgatatct tttttctttg aactataatt
     9181 cgatgacctt cggacgacat ccagacagct gcaagaaaaa acatggcctg gcaggataag
     9241 gagcgctgac attgaaaacg acaaattcaa tcctgtgtgt aatcgccagc gtcattgctg
     9301 ccggacaaaa tcaaatctca atcactgaac tggtaagcct ctccggtatt agtcgacaga
     9361 gtgttaagcg ggcaattcag atactggaac aggcagggca aatcaccgtt accagaacaa
     9421 ccaccaacgg caagcgcgga gccaacacct attacctgcc agaccaggat ttgggaggcg
     9481 gttgccgttg aacatggggt ccgct
//
LOCUS       DAZGTR010000150         9367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGTR010000150 DAZGTR010000000
VERSION     DAZGTR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.152"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(114..1602)
                     /gene="yeeO"
                     /locus_tag="ACHLCY_004225"
                     /pseudo
     CDS             complement(114..1602)
                     /gene="yeeO"
                     /locus_tag="ACHLCY_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            1763..1838
                     /locus_tag="ACHLCY_004226"
     tRNA            1763..1838
                     /locus_tag="ACHLCY_004226"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1796..1798,aa:Asn,seq:gtt)
     gene            complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="ACHLCY_004227"
     CDS             complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="ACHLCY_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP0371357.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2928..3845)
                     /gene="nac"
                     /locus_tag="ACHLCY_004228"
     CDS             complement(2928..3845)
                     /gene="nac"
                     /locus_tag="ACHLCY_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP0371358.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4172..4247
                     /locus_tag="ACHLCY_004229"
     tRNA            4172..4247
                     /locus_tag="ACHLCY_004229"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4205..4207,aa:Asn,seq:gtt)
     gene            complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="ACHLCY_004230"
     CDS             complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="ACHLCY_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0371359.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="ACHLCY_004231"
     CDS             complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="ACHLCY_004231"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP0371360.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKLGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="ACHLCY_004232"
     CDS             complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="ACHLCY_004232"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP0371361.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="ACHLCY_004233"
     CDS             complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="ACHLCY_004233"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP0371362.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(7828..8001)
                     /locus_tag="ACHLCY_004234"
                     /pseudo
     CDS             complement(7828..8001)
                     /locus_tag="ACHLCY_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(7980..8168)
                     /locus_tag="ACHLCY_004235"
                     /pseudo
     CDS             complement(7980..8168)
                     /locus_tag="ACHLCY_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         2628 a         2451 c         1981 g         2307 t
ORIGIN      
        1 gaactacaga ggaatcgtga gaacgaggcg aatattagcg atgcccaccc ataatgtcaa
       61 agcctgtttt ttaaatttga aatcgtttgc tgaaataatc tgcattttgt cgtttattcc
      121 gacacagctg gctttttttc acacttttgc ggttcggatc gagggtattt ccacagccaa
      181 cgtccagtaa ccattcgcca gtaaaacagc acggcccgca cagcccagtc ggcaaacatt
      241 cccatccaga caccaaccac accccagcca agcatgatgc ccagcacata accgacgaca
      301 acccggcaac cccacatgct caacatcgaa acccacatgg cgtaacgggc atcacgagca
      361 cctttaaatc cagcgggtag cacccatgag gcggaccaaa taggcataaa taaagcattt
      421 agccaaatca gaatcacaac gacatgttta acctgtggat cctgggtgta aaacgatgcc
      481 ataagcccgg caaagggagc cgttagccag gcgatggccg ttaatccaag agtggacagc
      541 cagaacacat gccgcaactg aatctctgct tgcgctatct gccctacccc caaccttcgg
      601 cctgtaatga tcgtagaagc agagccgagc gcacttccgg gtaagttgat aagagccgca
      661 attgaaaacg cgataaaatt tccggcaata acactggtcc ccatcccggc aacgaacatt
      721 tgggttaata accgaccact ggtaaataac actgattcga cactcgctgg aataccaatc
      781 caccatgact tcccagataa tgctaaaatt cagcggttta aaatagctct ttaacgaaat
      841 ccttagcgca ggattaaaac caatcgccag cacccacaaa attgcaactg cgccaatata
      901 acgagaaatg attaaaccca acccagcccc gacaaatccc agtcccggcc aggagaaaag
      961 gccgtaaatc aatatgccgc taataataat attaagaata ttcaggctac cgttaatcag
     1021 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaatca aagtgatggc
     1081 agctgctgga taactgagta ccgtcagctc cagataagtc aacgccagtg ctttaacttc
     1141 tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaataagcgt
     1201 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
     1261 tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg acagtagtac caagatcgat
     1321 agctgcaaaa aaagccataa tgaccatatt gaagctgtcc gccaatccca cgccggccat
     1381 cgcatctttt cccagccagc tgaccagaaa agtgctcagg acacccatca acaggacaca
     1441 ggcattctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt
     1501 gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca acctggcgta aagcagagga
     1561 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaata atgagggaga
     1621 atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga
     1681 tagaaacggc aaacagttgg ggaatttaaa aatcgggttt gacaaaagat ttttcgccgt
     1741 taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     1801 tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt
     1861 atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg
     1921 ccgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc gccagacata
     1981 gttacgctga agttgtcctc gcttcagtcc caaccagaca gtattagcat cgaaaagatg
     2041 ccgcgtatcc agacggatta aattctcttc ctcttgttcg ccgctggatt gttcggcaac
     2101 taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgcgcact
     2161 taatacaata tctgccagca aacctttgcg ggcaaatgcg tcatcaatac gtgagcgccc
     2221 cgtaatcccc tgtcggtaag tgattaacgg ccacttcgct attgattcca gcgtcaatgg
     2281 tgaaatttgc gtcaagggat gatcgtgtgg aacaagcaaa ctatggtgcc aacgaaacca
     2341 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     2401 ttcgccattt tgcaacaatg tcgcaatttc ctgtggcgtc ccctggatta gctcgagccg
     2461 aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg
     2521 agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag
     2581 ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc
     2641 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     2701 atcctccagt tcccgaatat gacggctgac gcctgactgt gaggtaaaaa gcatattcgc
     2761 aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa
     2821 attcacggta aactccgggc agttcagatt tcccgttatt gttaaagtct aatgcccggc
     2881 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacactta gctcaccaat
     2941 tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact
     3001 gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa agagagactc
     3061 atggaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgcg
     3121 gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
     3181 atagactcaa tttcaccaat aacttttgcc gtgagtcgcc gtagggaaaa agcctcatca
     3241 acacgaagtc taatagcact gtaatcactg gggagaaaga ggttcatttt cgcaatagca
     3301 ttcacatcaa cgctttgccc cgggcaatct tgagttccta ccagaaaaag atcttctttc
     3361 agcaaagcct gactggatac accagccaca ggggaatgct cataaatcac cgccatatcg
     3421 agttggtgat ttatcaattt ttcgttaagc actgcaccac tattttcatg aagatagata
     3481 acgatctccg gaaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca
     3541 gcggttcctg gtgcaaagcc aatcgagact tgccccgata atgcctgacc aacgttatgc
     3601 accgccagtt gggcctgttc acactgacgt aaaatggccc gcgcatgggt atagagaatt
     3661 tttccggcgt ctgttggcgt aacgccccgc tttgtacgga tcaaaagttg ttgatttaac
     3721 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     3781 gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     3841 ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagat
     3901 tgcaagatgc ttgccagttt tattttggcg ttgatgtaca agctaaccaa ctgtcaaata
     3961 agagattatg atagattcgt catttgctcc tttaatcagc tgtcgcgttc ccctgcccta
     4021 taaaaggagg gtatgcacca cgatgattca ttacccaata agattgaaag ctcaccactt
     4081 tgttgaaatt gacagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     4141 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     4201 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     4261 ctgctttcta gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga
     4321 accagaacta atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt
     4381 tacatcaact tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggtac
     4441 ttttcggtca gactcatatt ctgcacgatt gcgcgacagc ggctcatgaa cttccagcca
     4501 gttcgagcca tctggttcag tggtgtattt tactggctgg tcgataattt gcacacgcgt
     4561 tccaacagga acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc
     4621 ctgacttacc cggagcccaa taccaaaatt ggcattggta ccatggatgg catacaacct
     4681 gccaatataa atcgcgtaca gccccatggg attatcggga cccgcaggaa caaatgcggg
     4741 caaactctcc cctcgtttcg catattcgcg ccgagtgttc ggcgttggcg tccaggttgg
     4801 agcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     4861 gccgatacca ataggaaaga cttccacagt attactgtct ggcgggtagt aataaagacg
     4921 catctcagcg acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg
     4981 cggaatggta agttgcgagc cagacttcgg caagaaaaca tcagcacccg ggttcgcttc
     5041 cagcatgtta cttaaccctt gcccgttttg tgcggcaaaa gtctccagcg gctgggtatt
     5101 gtgatcagga acagttacag tatacgactg ccccactaaa cggctaccct ctggaggtaa
     5161 tggataagtt accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat
     5221 atttacacga cgcatcatgt ccctttccta agtcgcgaaa gctatccgtt aagtatagct
     5281 tttatcagac ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa
     5341 tattactggc agcaagttcg cccatgttgt tatatatcgc acaggcagct tcgatgatgg
     5401 gcatcgccag agctgcgcca ctcccctcac ctaaacgcat ctccatattg agataaggct
     5461 ccagccccaa atgcgacagc gcaatacgcg cccctttttc tgccgacagg tgagaaggaa
     5521 tgagatacgg tttgattgca ggagacatct ggcaggctgc gagcgcggca gcataagaaa
     5581 gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca
     5641 ttccgaccaa atcaaatcca cccaattttg ccaggacatc aacgccatcc tgaggatttg
     5701 gttgattcaa cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg
     5761 gcaggtttgc gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta
     5821 ttgctgctgc cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtaacac
     5881 cgttttttgc cagctcccgc gtataacata tgacgtccaa aagcaacttt tcagcctgat
     5941 ggcgactcat tgccggagct gaagcaatat tgccactacc tcgagcaaca cgcatgttga
     6001 taagcccggg gataggctca gcagtatcaa taccaacatc aattacgtgg acgttagcgc
     6061 ccgcttgtgc tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa
     6121 tggctgtcac ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac
     6181 acatgaccag taccgctttt ttgcccacat gcggaacgcc attcaacccc ggcattcctg
     6241 ccagttgtat ggcaagcgcc tccagccttc ccaggctacc aacaggtttg agtaacccgt
     6301 cgatatgccg ttgtgcacgc gacatagcgg caggatcgat ggcagggatc gtattcagta
     6361 aatcggcaag tgtttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc
     6421 accaagctct atagcagctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag
     6481 aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat
     6541 accaggcaat aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg
     6601 cccatcaatt ttgccaatga acacattgcc aagcccttct tcccgggcat aacgatgacg
     6661 atacatcaat aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag
     6721 gatcggttcg ccacgcagag ccaactcgct taataccaga atctttgcca gtacaacaaa
     6781 aatcaatgcc agaccgccgt gggtgcctaa acggctatca cgcatgattt ccaacatgcg
     6841 atcgcggcta cgtgcagaaa atacgccatc acaggtatcg gcgaggccgt ccaggtggaa
     6901 tcccccggtc atcagcgcca gcacaagtac gctaaacagt gccgccagtg gtacgccaca
     6961 ccatgcctgc agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaattaa
     7021 aggaaaagta ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg
     7081 cggtacaggc agacgcgtaa taaaagagag catcgcccaa aataatttac tcatttgatt
     7141 tttactccaa tacccgaaac caccagccat acttcatttg ccgcagcggc caactgctga
     7201 tttacccgcc cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc
     7261 attcccactt cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc
     7321 aacgactgaa tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg
     7381 tctttatcgc cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattcaagc
     7441 aacacaacct cattcgggtt aatgtctgca tgaattaatt catcaagatg ttgccagcgc
     7501 tccactgtgc gccagtgctc cgggcggcct tgccgatgat gttctatccg tgcagccatc
     7561 tcatcatcaa ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc
     7621 gcctctgcgt ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg
     7681 actcctgatt caaatgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg
     7741 ctgaccgcaa aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt
     7801 ctcaatccat tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt
     7861 ccgacaaaat gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg
     7921 aattaatgct actgccggaa caaccagcaa acgggcattg tgttctgaaa tccaggctat
     7981 tgattcaacg tcagcgacag atctgcgctg taaaagaact tgtaaaacaa cgtaatagaa
     8041 ttatccggaa tggtggcgac tatgcactag aacaccacaa tttcgctctc tcggtaaggc
     8101 actgtcacag cattcaacag aatgtgactt gccagattta ttagcgccac cagatgttta
     8161 accgggtaac cacgacccag tcggggatat attccatctt ctcgctgaca tcatcaagaa
     8221 taaaaaggtt tatcacactc agagcaacgt tcttcagcag gcgggataat tcttcaacat
     8281 ttagaagatg tgttattaaa ggcatacaaa ctggacgtat tgtattttct tttggtggtt
     8341 gacctaaggt agcagtttat cctgatgcgc tgagatttct gtaatatcta cgtcaacatt
     8401 ctcctcgaat agaaaatgca gccccggcaa gtaattcaca ttttttaaca aaacgttgtt
     8461 agcgtgccat tttcagaatc tctccaagaa agcggattct tttctcaaat tctgcattct
     8521 cagacacagc tttctactcc atggtaatgg cccaaatacg aagttgctca ggatcattag
     8581 tggtgctgag aagttaaata accatgcgga gtaccactcg tcatcacgta tttatatatc
     8641 atgaagctat tgttgtgttt tgtacatcag tagatatatt ctgatatact ccttttgcta
     8701 gacataacct ttcacctgct tgcaaagctt ctgtgttctg acattgccaa attgttgcaa
     8761 ttctgtatcc agccttcttt cagtcatagc ttcgggccgc gataagactc actgatctga
     8821 ccctgattcc tcttgcagac tttatagacc aattaaaatg cagtttctgc aggtcaacgt
     8881 ctgaccatca ttgtcatcac tctggccatt agagtaacct tctgcattca tccttttgta
     8941 aaaagtttat attagtatca gcaattaacc ggacctgata ctgatatgag tcttaccgca
     9001 tatacggtca atttcagcaa ttaattacat tatccacgcc aaagtatttg tcatcacaat
     9061 gatggtacct tctttcagac accatttttt caactccgtt ttccacggac cgcactctta
     9121 tgtcaagagt gcggtccgtg gatacaacca gagaccgact gacacgagtc agaggaaacg
     9181 acggatatgt tcagtcgtaa aatatctatc aaaaaacatg attaaggtca aaaatgtttg
     9241 atatttacaa tttatgaaga tgacaataat tatagatata tgagaacata aatgaaaata
     9301 attatcatta cagcaattat ttgtactttg tattaatgag ggatgaaatg ttatataata
     9361 taccttg
//
LOCUS       DAZGTR010000151         9161 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGTR010000151 DAZGTR010000000
VERSION     DAZGTR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.153"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(140..352)
                     /gene="cspA"
                     /locus_tag="ACHLCY_004236"
     CDS             complement(140..352)
                     /gene="cspA"
                     /locus_tag="ACHLCY_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP0371363.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(633..923)
                     /locus_tag="ACHLCY_004237"
     CDS             complement(633..923)
                     /locus_tag="ACHLCY_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP0371364.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1357..2067
                     /locus_tag="ACHLCY_004238"
     CDS             1357..2067
                     /locus_tag="ACHLCY_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP0371365.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2117..3091)
                     /gene="ghrB"
                     /locus_tag="ACHLCY_004239"
     CDS             complement(2117..3091)
                     /gene="ghrB"
                     /locus_tag="ACHLCY_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP0371366.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3195..3854)
                     /locus_tag="ACHLCY_004240"
     CDS             complement(3195..3854)
                     /locus_tag="ACHLCY_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP0371367.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4007..6340
                     /gene="bisC"
                     /locus_tag="ACHLCY_004241"
     CDS             4007..6340
                     /gene="bisC"
                     /locus_tag="ACHLCY_004241"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP0371368.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6309..6749)
                     /locus_tag="ACHLCY_004242"
     CDS             complement(6309..6749)
                     /locus_tag="ACHLCY_004242"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0371369.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQLAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6746..7309)
                     /gene="tag"
                     /locus_tag="ACHLCY_004243"
     CDS             complement(6746..7309)
                     /gene="tag"
                     /locus_tag="ACHLCY_004243"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP0371370.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7467..8165
                     /locus_tag="ACHLCY_004244"
     CDS             7467..8165
                     /locus_tag="ACHLCY_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0371371.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8394..>9161
                     /locus_tag="ACHLCY_004245"
     CDS             8394..>9161
                     /locus_tag="ACHLCY_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0371372.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAV"
BASE COUNT         2189 a         2253 c         2378 g         2341 t
ORIGIN      
        1 tctcattgca cccaaattta tttttcacaa aaataatact agattttatt acgcgatcga
       61 ttatttattt cctgaaaaac aaataaaaaa atccccgcca aatggcaggg atcttagatt
      121 ctgtgctttt aagcagagat tacaggctgg ttacgttacc agctgccggg cctttagcgc
      181 cgctttcgat ggtgaaggac actttctgac cttcgtccag agatttgtaa ccatcgttct
      241 ggatagcaga gaagtgtacg aacacgtctt tagagccatc gtcaggagtg atgaagccga
      301 agcctttgtc agcgttgaac cattttacga taccagtcat tttaccggac atagtgtatt
      361 acctttaata attaagtgtg cctttcggcg atatggcgtg ctttacagat tttgaagcgt
      421 taaaggaatg tgcactacga ggggtatcaa cgataactct tgaagggact tgccttacta
      481 cactgcttta atggtctgta cgtcaaaccg ttgatgtgca ttaagccacg cattggcggg
      541 tgatgcaaca attatttttc atatttatga ttaatcggtt ggaaaacggt cctgtcatca
      601 ggaccgtaaa cagcaataaa gtggataaaa gtctattcca tcaactgctt acttaatgcc
      661 gggttggctt gaatcaaacg catcaatttt agttcggcac ttgaaggctt cacgcgtctg
      721 gattcccatt cctttaccat ggcgactgat acgcccaaaa cccgggcgaa atcatcgatt
      781 tttaaccctg tcccttttcg taactgctca atttcggtac aggacgttgt tctgtgcgtc
      841 agggtaattt tctgcgtttc atctttaaaa acaatctgtt ccaggctgct caacagctca
      901 tgcattggat ctttatattc cattgaaaac tcctcaaatc acactgcggg atcgtgaatt
      961 gcatcgaagc tcattaagaa tagtcgggaa aagcagacac agcggggtga agtcagggca
     1021 tgctctggct atgattcgat tcagccagtc atttagcgtg acttatagcc acctaacgat
     1081 ctgataaatg ccgatatatc tcttaccact cctggtttgt aaagatttcg aaaatctaac
     1141 cttctgcgaa ggcgtaaatc tctggctaca aaaaaagcaa cggcactatc attttttaac
     1201 aatgaaacaa caaattaacc ttgtttcatt ttctccatga ttttaccagg aagattacgt
     1261 agaaagggta tcctgcaagc ctgtcctgga ctatccttgt cacgtcagac acgcgtgtgt
     1321 cgttgtgcgc tttttttggg tgaaaggagt aagaaaatgg cgacaggaaa gtcctgctct
     1381 cgctggtttg cgcctcttgc ggcgttatta atggtagtta gcctgagtgg gtgttttgat
     1441 aaagaaggcg atcagcgtaa agcgtttatc gacttcctgc agaatacagt gatgcgtagc
     1501 ggtgaacgtc taccaaccct gactgccgat cagaaaaaac agtttggtcc ttttgtctct
     1561 gattacgcga ttctgtatgg ttattctcag caggtgaatc aggcgatgga ttccggtctg
     1621 cgcccggtcg tagacagcgt taacgcaatt cgcgtaccgc aggattacgt tacgcaaagc
     1681 ggtccgctgc gtgaaatgaa cggttcgctg ggtgtgctgg cgcaacaact gcaaaatgcg
     1741 aagttgcagg ccgatgctgc gcactctgcg ctaaaacaga ccgatgattt gaaaccggtc
     1801 tttgatcagg cattcaccaa agtggtaacg acgccagctg atgcattgca accgttgatt
     1861 ccggctgcac aaactttcac gcaacaactg gtgatggttg gagactacat tgctcagcaa
     1921 ggtactcagg tgagctttgt cgcaaatggc atccagttcc cgacttcaca gcaggcaagt
     1981 gaatataaca aactgattgc gccattaccg gcacaacatc aggcgtttaa tcaggcctgg
     2041 actacagcag ttactgcaac ccaataaaga gtaaaagccc gagcgattat cgggctttac
     2101 ttcacgcagt cgcggcttag tccgcgacgt gcggattcac acagttcttc tcaacctttc
     2161 cttgtaacgc atcaatcaaa ttatccacgg cacaggcggc catgccataa cgcgtctcat
     2221 gggtggcaga tccaatatgc ggtactgcga cgacgttggc cattgagagc aacggcgaat
     2281 ctacggacag tggctcttgt tcgaagacat ccagcccggc ggcgtgaatt tcgcctttct
     2341 gcaatgcagc aatcagtgca ttttcatcaa ccaccgggcc acgtccggca ttaatgaaaa
     2401 tggcggagga tttcattttg gcgaattgtt ctgcgccaaa cagatgatgc gtctcatcag
     2461 ttaacggcag gatcaggcaa acgaaatctg actcttgtaa cagagtatcc aaatcgcagt
     2521 agcgggcgtt gaagcgttct tctgcttctt tatggtggcg gcgcgcgtta tagaggatgg
     2581 gcatgttgaa gccaaagtgc gcacgttgtg ccagcgccat accgatccgt cccatcccga
     2641 caatgcccag tgttttatgg tgaacgtcag tgccgtacca gtccgggcct atgctcgcgg
     2701 tccattcgcc tgcttttacc cgttctgcta cctccacaac ccgacgagcg gtagacaaca
     2761 ccagcgccat cagcgtatcg gcgacggttt ctgttaatac ggttggcgtg tgcatcagca
     2821 gaatttttcg ggcggtaagc gcatcgacat caaaattgtc atagccgacg gagatcgttg
     2881 atgtggcacg cagtttcggc attttttcca gcaatgcggc atcaacattc tcgtttgaac
     2941 ccagtaaacc ttcagcttcg gcaaaaattg ctgcattttg ttcgacggtt tgtgggctga
     3001 ggtttgccac ctggtgaacg gtgaaatgct cttgcaggcg ttgcagtaaa tcatcaggta
     3061 aggctttgta gaggataacg gacggcttca tgcttctctc cattcactga taaggcgatc
     3121 accttagcct gaaatatgat gaaggaaaac tgaagaatgc caggtgattt ctgcatcacc
     3181 tggcatgaaa gggattacag cgggcttaag gtaatttcta cacggcggtt ttgcgcctta
     3241 ccttctgcgg tgctgttgct ggcgattggg ttagccgggc caaggccctg agtacggatg
     3301 cggctggcgt ccacgccctg ggtgatcaac gcgctggcaa cggaatccgc acgttgctgg
     3361 gagagacgca tgttcaggtc gtgaccaccc gtgctgtcgg tataaccaat cacgttaacc
     3421 gccgttttcg gatactcttt cagcaccatt gccacgccgg tcagggtgtt agcgcccgca
     3481 ggtttcaggg tcgcgctgct gctgtcgaag gtcacattgt tcggcatatt gaggataatg
     3541 ttatccccgc tgcgggttac gctaacacca gtgccgcgca ttttgtcacg cagcttcgct
     3601 tcctgcacat ccatgtaata acccacgccg ccgcccagag ctgcgcctgc tgctgcgcca
     3661 atcagcgcgc ctttaccgcg atctttcttc gaagaagaga gcgcaccaat acccgcgccc
     3721 acgagagagc ccagacctgc gccgatagca gatttacctg cttcgcgttc gccggtgtaa
     3781 gggttagttg tgcagccaga taccgccaga gcgccactca ctacggcggc aataagataa
     3841 acacgtttct tcattgttaa tccttaataa cctttttatt ctttgccacg ggttccgtgg
     3901 cgggagatta tgccgcgtga acatgaagat tattcctggg aatactcgga aatttgtaag
     3961 taatatttaa ctgctcaata catctaacct ttcaggagtt ttcggtttgg ccaactcatc
     4021 ctcacgatat tccgttctga ctgccgccca ttgggggccc atgctggttg aaaccgacgg
     4081 cgaaaccgtg tttagctcgc atggcgcgtt agccacagga atggaaaact ccttgcagag
     4141 cgcggttcgc gaccaggttc acagcaatac acgggtacga tttccaatgg tgcgcaaagg
     4201 ctttcttgcg tcaccggaaa atccgcaagg cattcgtggg caggatgaat ttgttcgcgt
     4261 gagttgggat gaggcgctgg atcttattca ccaacaacat aaacgcattc gtgaggctta
     4321 tggtccggca tcgatttttg ctggttccta cggctggcgt tcaaacggcg tgctgcataa
     4381 ggcctcgaca ttattacaac gctatatggc gctggcaggc ggttataccg ggcatctggg
     4441 ggattattcg accggcgcgg cacaggcgat catgccgtat gtcgtgggtg gtagcgaagt
     4501 ttatcaacag caaaccagtt ggccgctggt gctggaacat agcgatgtcg tggtgttgtg
     4561 gagtgctaac ccactcaata cgctgaaaat tgcgtggaat gcatctgatg agcaggggct
     4621 ttcttacttt tccgcactgc gtgacagcgg gaaaaagctg atctgtattg atccaatgcg
     4681 atcggaaacc gtcgatttct ttggcgataa aatggagtgg gtggcaccgc acatgggcac
     4741 cgatgttgcg ctgatgctgg ggatcgccca tacgctggtg gaaaatggtt ggcacgacga
     4801 agcgtttctg acgcgttgca ccacaggtta tgccgtcttc gcctcttatt tgctgggcga
     4861 gagtgacgga atagcgaaaa acgccgaatg ggcggcagag atttgtgggg ttaacgcagc
     4921 gaaaatccgc gagctggcgg ctcttttcca ccaaaacacg accatgctga tggctggctg
     4981 gggaatgcag cgccaacagt ttggtgagca aaaacactgg atgatcgtca cgctggcggc
     5041 aatgttgggg caaatcggca cacccggcgg cggttttggt ctttcttatc attttgccaa
     5101 tggtggtaac cccacgcggc gttctgcggt gctctcttcc atgcagggca gcttgccggg
     5161 tggctgcgat gcggtggata aaatccctgt tgcccgcatt gttgaagcac tggaaaaccc
     5221 tggtggcgca tatcaacaca acggtatgaa ccgacatttc ccggatattc gttttatctg
     5281 gtgggcgggc ggtgccaact ttactcatca tcaggatacc aatcgtctga tccgtgcctg
     5341 gcaaaaaccg gagctggtgg tgatctctga atgcttctgg acggcggcgg cgaaacacgc
     5401 ggatatcgtt ctgcctgcga ctacctcttt tgagcgtaat gatctcacca tgaccggtga
     5461 ttacagtaat cagcatctgg tgccgatgaa gcaagtggtg ccgccacgct atgaagcgcg
     5521 taatgatttt gatgtttttg ccgagttgag tgaacgttgg gagaagggcg gctatgcgcg
     5581 gtttacggaa ggaaaaagtc agctgcaatg gctggaaacg ttttataacg ttgcccggca
     5641 gcgcggggca agccagcagg ttgaattgcc gccatttgct gagttctggc aagccaacca
     5701 gttaattgag atgccggaaa acccggacag cgagcggttt attcgcttcg ctgatttttg
     5761 ccgcgatccg ctggcgcatc cgttaaaaac cgccagcggc aagattgaaa tcttctcaca
     5821 gcgtattgcc gattacggtt acccggattg ccctgggcat ccaatgtggc tggagccgga
     5881 cgaatggcag ggcaatgccg aaccagaaca gttgcaggta ctttctgccc atccggcgca
     5941 ccgcctgcac agccagctga attacagttc tctgcgcgaa ttgtacgcgg tagcaaaccg
     6001 tgagcctgtc accattcatc ctgacgatgc tcaggcgcgc ggcataaccg aaggggatat
     6061 ggtgcgggtg tggaactccc gtgggcagat ccttgccgga gcggtcatta gcgagggaat
     6121 taaacctggc gtgatttgca ttcacgaagg ggcatggccg gatctggatt taaccgctga
     6181 cggtatttgt aaaaacggcg cagtgaacgt gctgaccaaa gatctcccca gctcgcggct
     6241 ggggaatggc tgtgcgggta atacggcgct ggcatggctg gaaaaataca acggtccgga
     6301 actgacactt acagcgtttg aaccaccggc cagctcataa tccaggtggg tagttgggtt
     6361 tcatcctgcc atgcgcaatc gacaatgtga aaaccctgcg cacggtaaaa atctatcgcc
     6421 agttgatttt tttgataaac ctccagcatc agatggggat agcgctgctg cacatactgc
     6481 atcagcgcct taccaatacc gcgtctgacg gccttcggtg cgacaaacat tgctgccaga
     6541 aatcggcctt ccataatgct gacaaaaccg agaagcttac cgtcctcttc ccagacccag
     6601 ttttgcgcgt tggcaagata ggcatcccgc accagcggaa tgcagtcacg ccagtaattc
     6661 gcttttataa agggatgccc ccaggttgta ctttccagcc acagttcgag gatcgcgggg
     6721 agttctgaac gttgcgcttc ccgaatcatg gtttatttcc cggatagcaa cagcagccaa
     6781 ccacatgatc attcaccagc ccacatgcct gcataaagga gtaacagatt gtggtgccga
     6841 caaacttaaa accacgtttt ttcagtgcct tagatagggc gtcggaggcg gacgtagatg
     6901 tgggaatttc gctcaacgtt gtggcttgtg tcacctgtgg ctgatgattt acaaacgacc
     6961 agacaaagtc ggcaaacggt tcgccgttct gttccatttg caggtacgcc cgcgcattac
     7021 caataattgc ctgaattttc cctcgatggc ggataatccc ggcgtcctgt accagtcttt
     7081 cgacatcctc ttcctgcatt gctgcgacct tcaccggatc gaactgatga aagcaggcgc
     7141 gatagttttc gcgttttttg aggacggtga tccacgataa cccagcctgc tgcccttcaa
     7201 ggcagatcat ttcgaacagt tttttactgt cagtttcagg cacgccccat tcattatcat
     7261 ggtaggcaat ataaagcggg tcctgactca cccagccgca acgttccata ctttccctcg
     7321 ctatgtaccc gatcgtaaaa atttcagccg cttttcttga cgcatcggtg aaaaagtcaa
     7381 tatttaatac agggctacta tgcccgatat ccttcatgca atgacaataa tattgccgaa
     7441 ttcggctctt ccctggagtc gtgttaatga tcataaaaaa aagcggtggt cgctggcagc
     7501 taagcctgct ggcgagcgtg gtaatcagtg ccttttttct caacacagct tacgcctggc
     7561 aacaagaata tatcgttgat acgcaacccg gacattccac agagcgttac acctgggata
     7621 gtgatcatca acctgattac aacgatattt tgtcgcaacg tattcaaagt agccaaaggg
     7681 cgctgggact ggaagtcaat ctggcggaag aaactcctgt ggatgtgacc agcagtatga
     7741 gtatgggctg gaattttcct ttatatgaac aggttacaac cggcccggtc gcggcattac
     7801 attacgatgg cacaaccacc tcgatgtata acgagtttgg cgacagtact accacgctga
     7861 ccgatccgtt atggcatgcc agcgtgagta gcttaggctg gcgtgttgac tcccggcttg
     7921 gcgatctccg accctgggcg caaatcagct ataaccagca atttggcgag aatatctgga
     7981 aggcgcaatc aggcctgagc cggatgacgg cgacaaacca gaacggcaac tggctggatg
     8041 tcaccgtagg cgctgatatg ttgctcaatc aaaatattgc cgcctatgcc gcgctaactc
     8101 aggcagaaaa taccactaat aatagcgact atctgtatac gatgggggtt agcgccagat
     8161 tttaacgtaa cagtcacaat tgaaaccatt gaataacaac agttgtggcg atagtgggtg
     8221 ctaacttacc aaataataaa tttggtgaat aattgtcgcg tcattcattc ctgaactaag
     8281 gcatttcatt ccgttctgat ggcatttcat gccgtttttc cccaggcata aaatgcactt
     8341 cgttatggtt gtcggcagag atttttcctt tttattactg caggaatact gccatgacac
     8401 cttcaaatta tcagcgtacc cgctggctga cactcatcgg tactatcatt acccagtttg
     8461 cactggggtc ggtttatacc tggagcctgt ttaatggcgc gctttctgcc aagctggatg
     8521 cgccggtaag ccaggtcgct ttctctttcg gcttgttaag tctggggctg gcaatttcgt
     8581 cttctgttgc gggcaaattg caggaacgtt ttggcgttaa acgcgtcacc atggcttccg
     8641 gcattttgct gggattaggc ttcttcctga ccgcgcattc caacaacctg atgatgctgt
     8701 ggttaagcgc cggtgtgctg gtcggtctgg cggatggcgc gggttacctg ctgacgctct
     8761 ctaactgcgt gaagtggttc ccggagcgta aaggtctgat atccgcgttc gctatcggtt
     8821 cttatggtct gggtagcctg ggtttcaaat ttatcgacac gcagctgctg gaaacggtgg
     8881 gtctggaaaa aacctttgtg atttggggag cgattgcgct ggtgatgatt gttttcggcg
     8941 caacgttaat gaaagacgca ccaaaacagg aagtgaaaac cagcaatggt gtggtggaga
     9001 aagattacac gctggcagag tcgatgcgta aaccgcagta ctggatgtta gcggtaatgt
     9061 tcctgaccgc ctgcatgagc ggcctgtacg tgattggggt agcgaaagat atcgcccaaa
     9121 gtctggcaca ccttgatgtg gtttccgcag ccaatgcagt c
//
LOCUS       DAZGTR010000152         8993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGTR010000152 DAZGTR010000000
VERSION     DAZGTR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.154"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(13..438)
                     /locus_tag="ACHLCY_004246"
     CDS             complement(13..438)
                     /locus_tag="ACHLCY_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0371373.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(422..703)
                     /locus_tag="ACHLCY_004247"
     CDS             complement(422..703)
                     /locus_tag="ACHLCY_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFP0371374.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQMLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLTRSLIHENQA"
     gene            803..1222
                     /locus_tag="ACHLCY_004248"
     CDS             803..1222
                     /locus_tag="ACHLCY_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371375.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            1488..1640
                     /locus_tag="ACHLCY_004249"
     CDS             1488..1640
                     /locus_tag="ACHLCY_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0371376.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNSD"
     gene            1652..2290
                     /locus_tag="ACHLCY_004250"
     CDS             1652..2290
                     /locus_tag="ACHLCY_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371377.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLDKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2291..2500)
                     /locus_tag="ACHLCY_004251"
     CDS             complement(2291..2500)
                     /locus_tag="ACHLCY_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371378.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            3071..3259
                     /gene="dicB"
                     /locus_tag="ACHLCY_004252"
     CDS             3071..3259
                     /gene="dicB"
                     /locus_tag="ACHLCY_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0371379.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            3256..3447
                     /locus_tag="ACHLCY_004253"
     CDS             3256..3447
                     /locus_tag="ACHLCY_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0371380.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(3480..3680)
                     /locus_tag="ACHLCY_004254"
     CDS             complement(3480..3680)
                     /locus_tag="ACHLCY_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371381.1"
                     /translation="MFQELPVKFCAGATFITGFNGSGYSDCTFFLYCLFVRNGGANKD
                     FTHFVLPHYRFKGEQIPAIAGI"
     gene            3585..5165
                     /locus_tag="ACHLCY_004255"
                     /pseudo
     CDS             3585..5165
                     /locus_tag="ACHLCY_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
     gene            5163..5927
                     /locus_tag="ACHLCY_004256"
                     /pseudo
     CDS             5163..5927
                     /locus_tag="ACHLCY_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5980..6069)
                     /locus_tag="ACHLCY_004257"
     tRNA            complement(5980..6069)
                     /locus_tag="ACHLCY_004257"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6033..6035),aa:Ser,seq:cga)
     gene            6163..6960
                     /gene="mtfA"
                     /locus_tag="ACHLCY_004258"
     CDS             6163..6960
                     /gene="mtfA"
                     /locus_tag="ACHLCY_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP0371382.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            7061..7136
                     /locus_tag="ACHLCY_004259"
     tRNA            7061..7136
                     /locus_tag="ACHLCY_004259"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7094..7096,aa:Asn,seq:gtt)
     gene            7316..8590
                     /locus_tag="ACHLCY_004260"
     CDS             7316..8590
                     /locus_tag="ACHLCY_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0371383.1"
                     /translation="MSLTDTKVKNARPAEKAVKLTDGFGLYLLVHPNGSKYWQLGYRF
                     DGKQKVFSIGVYPAVSLADARQRRDEAKKLLAQGVDPNAKKLADEKALQEKRDKTCSF
                     RVVARSWFATKTKWSEDYADTVWKRLETYVFPDIGDSNVSDLDTGDLLVPIKNAETLG
                     YLEIAMRIKQYITAILRHAVQQKLMRHNPAYDMEGAVQKPETEHRPALELEEIPLLLE
                     RIDAYKGRGLTTLAIKLNLLIFIRSSELRYARWSEIDFNSKLWVIPEKREAIERVKYS
                     TRGAKMKRQHFVPLCRQALKILKEIRQLTYEEGNEAGLIFTGCYDSFKPMSENTINKA
                     LRKMGYDTTQDICGHGFRTLACSALIESGLWSEDAVELQMSHKESNSVRAAYTHKAKH
                     LEQRRLMLQWWADYLDANRDGMVRPFEFAQKQ"
     gene            complement(8660..8911)
                     /locus_tag="ACHLCY_004261"
     CDS             complement(8660..8911)
                     /locus_tag="ACHLCY_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371384.1"
                     /translation="MSKNVKQTSENVASTAAKTLTNPNASAIQKSLAGSALSQRGTSN
                     QTSGQMEHKASSALDNPRSSKLTKQLAASVLAQSNKGRK"
BASE COUNT         2616 a         2011 c         2098 g         2268 t
ORIGIN      
        1 ggctattgtc agtcaatgta ctgccacagc atttcctgcc gggccaccac ggttcatctg
       61 attgaaaccg gcgattgcca ctgcgacaaa atcatcagcg tctctcacca gtcgctcccg
      121 cgtctccacc agctcccgaa aataagctga actgtggctg cgcattctgg ccaccagcaa
      181 aggtggcatt gccttttcga tcgctggtaa caacgcctga attttttcaa ctgcatcagg
      241 ggtgtctttc tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg
      301 gctgtcgtca tacagttcag gaaacgtcat acccaactca aaataagcct gggttattcc
      361 agctgctgga actttttcgc catcaggacg cgcccaggca ttcatcgcca tgcggatgtg
      421 ttcatgcttg attttcatga atcaagctcc tagtaagtgg ttgtgttaac gttttggtat
      481 cttccagctc gggccaaata ttcatccaat caaaaggcct tagttgctga cgtgtaactt
      541 caccattact ggctcgctca ataaggacac ataacgatgc ccctaacatt tgacctttac
      601 tcaatgcctt tcttagataa ccgatgctag taccacactc acatgcaaac atacgctgtt
      661 catctgacga aagagaattg agaaatattc ttaattcttc catagctact ccttagtaaa
      721 cacagcaaga atacccacag gtaaacaaaa gtcaataccc ttgggttgtt taccttgcgg
      781 taatcgcatc tattatttac ctatggacaa atatgaattt agacgacaac aactcatcaa
      841 aattcgtgat gagaaatgcg atggtaaagc ggttaacgtg gccagaaaga tcggtcgcga
      901 gccttcttat gtatcaagaa tgttgtaccc agaggggaaa aaaggaaaaa aacggatcgc
      961 tgatgatatg gtggagatta tagaagagtc ctttgggtta ccccggggat ggatggatgg
     1021 tatcgtttca tcatcaacga acacagcctc caattatgaa acaagggttc taacgccacg
     1081 acaacgtatt tttttagatc tcttagacga actgccagaa agtgaagcgg ataacttatt
     1141 aaaaactctt gaagagaaaa aacagtatta caatatgatc tacgaagaaa tccgtaaaaa
     1201 gaaagcacaa aacgcatcat aactcaccaa acaaccagtc accagttaag acacatcaaa
     1261 aaattaccca tgggtattta ctttttaaat acccatgggt atccttcctt tcataccaac
     1321 ccaccccgtc ccacagaacg cagggcaata cttcgagtta ccaggcagtg gtcagggggt
     1381 aagtagccag cccgaggcgt aagaacatga cggcagggtt caactttaat aactatgcag
     1441 caggtttttg ttccgctacc ccggcgttaa ggggaaatga ggtcagcatg gatactatcg
     1501 atcttggcaa cagcgaatct ctggtatgcg gcgtgtttcc caaccaggac ggtacgttta
     1561 ccgcgatgac gtataccaaa agtaaaacgt ttaaaaccga agctggcgcg catcgctggt
     1621 tagcaagaaa ctccgactga tgaggttgac gatggaattt aaagatttac ctccttcaat
     1681 ccaggagatt gcagcacaca cacttcgtca tcgtctgaac gaacttgaat tggaatcggt
     1741 aacgaaaaaa gacactgata atatggctcg taatgtgcgc gatgcgttta ccggattgta
     1801 tttctgtgcg tctataaata aacacgactc agagagtgtg gcaaataaaa ttgcagaaac
     1861 gacagcgcaa aacatcaata cgaaaccaac ggaagaagaa attgatcagt ttgctcatga
     1921 tgctggttta aaaaacaaga aagaaaaatc gccatatgcg gggaacatgt ttgtttatga
     1981 caatctcatc agaattcgtg gcgaaattcc ggcggaatac ctggcaagag tccatcaggc
     2041 attgcttaaa aatttggaaa cagaattatt tgatggcaac actaacggtt tcttcatggt
     2101 atcaggcctt gataaagact gggatgcaga aaaacgctgg aatgttgcta catggttatt
     2161 cagtaacaga gccgctgcac tggaagcttc ggcatgtatt tgcggcctgt tcttaacaga
     2221 ccacaaatat aatctggatg tgtacagtta tatttacgct gaacacggtc cactctggat
     2281 tgactggtaa ttataaggaa acaccagcag ggccgcggcg accaacagaa cgattaaaat
     2341 caataatgcc attataaagg acattattta atttatcgtc gaatgctgat tctgtgagcc
     2401 tcaactctga atgagttttt aataaccctg attgcctgag ttgatttacc aggcattcaa
     2461 tctgtttttc aataagcgga tttctttttt tgtttggcat tttatcctcc attgaggttc
     2521 tgggttaaaa atggagacca acacgctgtc acgtgtggtc gtgcgccgga cacggataag
     2581 aatccggtac tgacagttta ctgaaaggat atttccctga aaagtcaggg cataacgcga
     2641 aagcgtacgg cgaagctctt tcccttagaa ggcttgtcgt tagatttctt cgaacgtgcg
     2701 cttccggttg tggcactccg cgaaatggcg cggcggtaag tatggcgggg ttattccttc
     2761 cccgttgagg acaccgggtt gtcaggttga ccatacgctt aagtgacaac cccgctgcaa
     2821 cgccctctgt tatcaatttt ctggtgacgt ttggcggtat cagttttact ccgtggctgc
     2881 tctgccgccc tttttaaagt gaattttgtg atgcggtgaa tgcggctaag cgcacgcgga
     2941 acagttaaaa caagcggtct tttacgggcg taacgggaat gctctgtatc cggcgttaat
     3001 tgttaactgg ttaacgtcac ctggaggcac caggcactgc atcacaaaat tcattgttga
     3061 ggacgcgata atggaaacgt tattaccaaa cgttaatacg tctgaaggtt gttttgaaat
     3121 tggtgtcact atcagtaacc ctgtatttac tgaagatgcc attaacaaga gaaaacacga
     3181 acgggagcta ttaaataaaa tatgcattct ttcaatgctg gcccgtttac gtccgataca
     3241 aaaaggatgc tggcaatgaa tacagcattt gcacttgttc tgacagtttt tcttgtttcc
     3301 ggagagccag ttgatattgc agtcagtgtt cacaggacaa tgcaggagtg tgtaactgca
     3361 gcaaccgaac agaaaattcc cggtaactgt tacccggtcg ataaagttat tcaccaggat
     3421 aataacgaaa tcccggcagg tctttaaaac agttccgtaa taaacatccg atttcattct
     3481 tatatgccag caatggcagg gatttgttca cccttaaatc tgtaatgagg taaaacaaaa
     3541 tgagtaaagt ctttatttgc gccgccattc cggacgaaca ggcaataaag gaagaaggtg
     3601 cagtcgctgt agccactgcc attgaagccg gtgatgaatg tcgcgcccgc gcaaaatttc
     3661 actggcaatt cctggaacat tatccggctg ctcaggactg cgcttataaa tttattgtct
     3721 gcgaggataa acctggcata ccccgccctg ccctcgattc atgggatgct gaatatatgc
     3781 aggaaaaccg ctgggatgag gagtctgctt cttttgtccc ggttgagact gaatcctatc
     3841 cgatgaacgt cacttttgac aagctggccc ctgaagtaca gaacgctgtc atggttaagt
     3901 tcgacacatg tgaaaacatc accgttgata tggttattag cgcacaggaa ttgttgcagg
     3961 aagacatggc aacattcgac ggacatatcg ttgaagcgtt gatgaaaatg ccagaagtta
     4021 acaccatgta tccggagctt aagttgcacg ccattgggtg ggttaagcat aaatgtattc
     4081 ctggtgctaa atggcccgaa attcaggcag agatgcgcat ctggaaaaaa cgtcgcgaag
     4141 gtgaacgcaa ggaaaccgga aaatacacgt ctgttgttga tctcgcccgc gccagagcca
     4201 atcaacagta cactgaaaat tcaacaggaa aaatcagccc ggtcattgct gccattcatc
     4261 gcgaatacaa gcagacatgg aaaacactgg atgacgaact ggcctacgct ctctggcctg
     4321 gtgatgtgga tgccggaaac attgacggca gcatccatcg ctgggcaaaa aatgaagtta
     4381 tcgacaacgg ccgcgaagac tggaagcgta tctcggcatc aatgcgcaaa cagcctgatg
     4441 cccttcgcta cgaccgccag actatttttg gccttgtccg tgaacgtccg atcgacattc
     4501 acaaagaccc tgtggcactg aacaaataca ttactgaata cctgactaca aagggcgtgt
     4561 ttgaagatga aggaacaaat cagagcgcaa ctgacactct ctcgtcgcca gtaccagaaa
     4621 ctgatgcagt ggaaacggca attccggaca acgaaaaaac cgaatgcaaa gtggaagtcg
     4681 aaccatctgt agagcgtgag gggccgttct acttcctctt caccgacaag gatggcgaaa
     4741 aatacggtcg cgcaaacaaa ctttctggtc tggataaggc gctgtctgct ggggctactg
     4801 aaatcacgaa agaagaatat ttcgcccgca aaaacggtac atactcaggt tcacaacaaa
     4861 atactggtgc atctgacacg accgcacaac cagggtcagt aaaagttacc gctgacgaag
     4921 taaacaaaat tatgcaggca gccaatatca gccagcctga cgccgatgaa ctgcttgcag
     4981 tatcacgtgg tgaatttgtt gaagggatta gcgacccgaa tgatccgaaa tgggttaagg
     5041 ggatccagac ccgcgattct gtgaaccaga accagcaaga aacggaacag aacgaccaga
     5101 aagcggaaca aaacagccca aatacgcaac aaaacgagcc agaaacgaaa caacctgaac
     5161 cactacaaca tcaaaaaaag ttgcttgccg tattcggtaa ggtgccggca gattccataa
     5221 aaccagaaca catccgtcga tacatggaca agagagggga gcagagtaaa acgcaagcca
     5281 accatgaaaa aagcagtatg tcccgcgttt acagttgggg gtatgagcga gggtacgtga
     5341 agggtaaccc atgtgcaggt gtaagtaaat tcaaggccaa aaaccgcgaa cgatatgtaa
     5401 ccgacaaaga ataccaggca gtattaagcg ttgcacctct tcctgttttt atcgcaatgg
     5461 aaattgccta tctgtgtgca gcgagggttt ccgatgtgtt atcgctgaaa tgggagcaga
     5521 ttggaaacga cggaatcttt atccagcaag ggaaaacagg aaaaaaacag ataaaagcat
     5581 ggagtccacg attacaggcg gcgatcgaaa aagcaaaaca gttaccaaca tccgcctatg
     5641 taatcagtaa tcaatacggc aaccgatata tgtacaaagg ctttaacgaa atgtgggtag
     5701 aagcaagaaa tcgcgcaggc aaaatttcag gtattttaac cgacttcacc tttcatgatc
     5761 tgaaggcgaa aggaatttca gactatgaag gaagcagtcg ggataagcaa cttttctctg
     5821 gtcacaaaac cgaggggcaa gtgctaatct atgacaggaa ggttaaagtt tcaccgacac
     5881 ttgatgtccc gttacctgaa aatattccaa gaaaatattc caagtaattc caagtgtgat
     5941 ttttgtcact gacttaatga tgtataagtg attgaatttt ggcggagaga gggggatttg
     6001 aacccccggt ggagttgccc ccactccggt tttcgagacc ggtccgttca gccgctccgg
     6061 catctctccg ttcagatggt tgccatgatg ccaggaaatt tggcatttta acagtccctg
     6121 tccgtgcaat tttgttcaag tgacgagttt gcgagcaaaa cgatgattaa gtggccctgg
     6181 aaagtacaag aatcagcaca tcaaactgcc cttccctggc aggaagcact atcgatcccc
     6241 cttttaacgt gtctgacgga acaggaacaa agcaaattag tcactcttgc cgaacgtttt
     6301 ttacagcaaa agcggcttgt tcctttacag ggctttgaac tggattcatt aagaagctgc
     6361 cggatagcac ttctattttg cctacccgtt ctggagttag gactggaatg gctggatggt
     6421 tttcatgaag tcttaattta tcctgcgcca tttgtggtcg atgatgaatg ggaagacgat
     6481 atcggtctgg tgcataacca acgtattgtt cagtcaggtc agagctggca gcaagggcct
     6541 atcgttttga actggttgga tatacaagat tcttttgatg cttctggttt taacctgatt
     6601 attcatgaag tcgctcataa gctggacacc cgtaacggcg atcgcgccag cggagttccc
     6661 tttattccgt tgcgtgaggt tgctggctgg gaacacgatc ttcatgctgc aatgaacaac
     6721 attcaggaag aaatcgaatt ggttggcgag aatgcggcga gcattgatgc ttatgctgcc
     6781 agtgatcctg ctgaatgttt tgccgtactt tctgaatatt tctttagcgc cccagaactt
     6841 tttgctcctc gtttcccttc attgtggcaa cgtttctgcc aattttatca acaagatcct
     6901 ttgcagagac tgcatcgcac taatgataca gactcgtttt cggcgacgaa tgttcattaa
     6961 ttaacaactt tgcagattaa ttaaccaatt gaaatgactt atgaaattta gtgttgacag
     7021 acaaggtacc gctaagtaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt
     7081 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaattt
     7141 aagggaagca gacgttcact gacgtctgct ttctgcattt atatcaactg gttactccct
     7201 tcttcaggtt caccctcgtt cgttaaaaat cgctcgaacc cacactactt tgctggtaaa
     7261 aatgctggta aagctggttc gatttgcgtt ttaccagcac gcggagggaa ccgtcatgtc
     7321 acttactgat actaaagtaa aaaatgccag accagcggaa aaagccgtca agctcactga
     7381 tggatttggt ctctaccttc ttgtgcatcc caatggttca aaatactggc agttaggcta
     7441 tcgcttcgat ggtaaacaga aagtgttttc cattggtgtt taccctgcgg tttctcttgc
     7501 tgatgccaga caacgacgtg atgaagcaaa aaagctgctt gctcagggag tcgatcctaa
     7561 cgctaaaaaa ctggctgatg aaaaagcgct gcaggaaaag cgggataaaa cctgttcgtt
     7621 ccgtgtcgtc gccagaagct ggtttgccac caaaacaaaa tggtcagaag attacgccga
     7681 tacggtatgg aagcgccttg agacctatgt attcccggat attggcgaca gcaacgtttc
     7741 agatctggat acgggtgatc tgcttgttcc gattaaaaat gcagaaacac tcggctacct
     7801 tgaaattgcc atgcggatta agcaatacat caccgcgatc ctacgtcatg ccgtccagca
     7861 aaagcttatg cgtcataatc cggcctatga tatggaaggc gctgtccaga aaccagagac
     7921 ggaacaccgc cctgcactgg agctggaaga gatcccgcta ctgcttgaac gtattgatgc
     7981 ctacaaaggt cgtggactaa ctacactggc gattaaactc aatctgttga ttttcattcg
     8041 ttccagtgaa ctccgctatg cacgatggtc agaaatcgac ttcaacagta agttatgggt
     8101 aataccagaa aagcgtgaag cgattgagcg cgtcaaatat tccacgcgtg gcgcaaaaat
     8161 gaaacgccag cacttcgttc ccctctgcag gcaggctctt aaaatactga aagagatccg
     8221 ccagcttacc tatgaagaag gtaatgaggc ggggttaatt tttacgggct gttatgactc
     8281 gttcaagccc atgagtgaaa acaccatcaa taaagcgcta cgtaagatgg gctatgacac
     8341 cacgcaggac atctgcggtc atggtttccg cacgctggca tgtagtgctc tgattgaatc
     8401 agggctatgg tctgaagatg cggtagaact tcaaatgagc cacaaggaaa gcaacagcgt
     8461 tcgtgctgcc tatacccata aggccaaaca tcttgagcag cgaaggttga tgctacagtg
     8521 gtgggcagat tatcttgatg caaaccggga tgggatggtc aggccgtttg agtttgctca
     8581 gaaacaataa tcgccttcca gcaagcagac aatcgtagta aataaaaaac gcagggaggt
     8641 ttactctccc tgtaatttgt cacttacgcc ctttgtttga ttgtgccaaa acagaggctg
     8701 caagttgctt cgttaacttg ctggaacgtg gattatcaag agctgatgat gccttatgct
     8761 ccatttgccc acttgtctgg tttgatgtcc cacgttgtga aagcgcggaa ccagcgaggc
     8821 ttttctggat agctgaagcg ttcggattag tcagcgtttt tgcagcggta gaagccacat
     8881 tttcagaagt ctgttttacg tttttactca tgaaacgaat ccccaagaag ttatgaatat
     8941 tccttatgca gtactttcat ttctaaaaaa acactatatg aagtgataac cat
//
LOCUS       DAZGTR010000153         8979 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGTR010000153 DAZGTR010000000
VERSION     DAZGTR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.155"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(58..609)
                     /gene="ymfL"
                     /locus_tag="ACHLCY_004262"
     CDS             complement(58..609)
                     /gene="ymfL"
                     /locus_tag="ACHLCY_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP0371385.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPELEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            complement(602..862)
                     /locus_tag="ACHLCY_004263"
     CDS             complement(602..862)
                     /locus_tag="ACHLCY_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0371386.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNTATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEISRG"
     gene            960..1652
                     /locus_tag="ACHLCY_004264"
     CDS             960..1652
                     /locus_tag="ACHLCY_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0371387.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDLKSNTVCKNSISEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            2356..2718
                     /gene="yfdP"
                     /locus_tag="ACHLCY_004265"
     CDS             2356..2718
                     /gene="yfdP"
                     /locus_tag="ACHLCY_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP0371388.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            2784..3608
                     /gene="yfdQ"
                     /locus_tag="ACHLCY_004266"
     CDS             2784..3608
                     /gene="yfdQ"
                     /locus_tag="ACHLCY_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP0371389.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLTEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            3736..4272
                     /gene="yfdR"
                     /locus_tag="ACHLCY_004267"
     CDS             3736..4272
                     /gene="yfdR"
                     /locus_tag="ACHLCY_004267"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0371390.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPSHA
                     YGMFMERFNELSELHKCA"
     gene            4263..4613
                     /locus_tag="ACHLCY_004268"
     CDS             4263..4613
                     /locus_tag="ACHLCY_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eae protein"
                     /protein_id="HFP0371391.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDEVRAEARN
                     EGINYTASRLAAAFNHGFINKPLAEVFDVTRMILSAKEELANESHPIDGLSGEYAEKS
                     LEEWAERLRKGGSQ"
     gene            4610..5083
                     /locus_tag="ACHLCY_004269"
     CDS             4610..5083
                     /locus_tag="ACHLCY_004269"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0371392.1"
                     /translation="MTGHAAILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFSMVIFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLRPSGVLIFKWNETQIPVRQILVLTDRKPVIGQRTGKNDKTHWIIFMK"
     gene            5230..5646
                     /locus_tag="ACHLCY_004270"
     CDS             5230..5646
                     /locus_tag="ACHLCY_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371393.1"
                     /translation="MAELTKWLQNTITGIETVVDDKSFVCDEIVFKIDVVKNVLTAFK
                     VALVSLEAEPVTWRYRYVRKGVTNFQEKPWVGGWKYVPTKEDCNDRPNYEIQALFTAQ
                     PVPLTPEGLIKAVRFYEQVKSENPPVETGAWKDAVD"
     gene            5699..5890
                     /locus_tag="ACHLCY_004271"
     CDS             5699..5890
                     /locus_tag="ACHLCY_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371394.1"
                     /translation="MNGQISIVRPGACDDREIRMIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNVEIKVK"
     gene            5893..6189
                     /locus_tag="ACHLCY_004272"
                     /pseudo
     CDS             5893..6189
                     /locus_tag="ACHLCY_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310783.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            6436..6627
                     /locus_tag="ACHLCY_004273"
     CDS             6436..6627
                     /locus_tag="ACHLCY_004273"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0371395.1"
                     /translation="MPEKGQNVLISVNFDSSLVEPLICSARYTGSTFRRGDATIKPGN
                     GIEQATHWMPLPEPPQEVK"
     gene            6627..7199
                     /locus_tag="ACHLCY_004274"
     CDS             6627..7199
                     /locus_tag="ACHLCY_004274"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0371396.1"
                     /translation="MNNLMIDLETMGKNKDAPIVSIGAVFFTPETGDIGQEFYAVVSL
                     DSAMKQGATPDGDTILWWLKQSPEARAAICIDDTLSISDALSELNHFINRHADNTKYL
                     KVWGNGATFDNVILRGAYERAGQICPWAYWNDHDVRTIVTLGRSIGFDPKMDMPFDGE
                     RHNALADARHQAKYVSAIWQKLIPATSTEL"
     gene            7236..7517
                     /locus_tag="ACHLCY_004275"
     CDS             7236..7517
                     /locus_tag="ACHLCY_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFP0371397.1"
                     /translation="MNTLFLLMAEFNTPNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWEQVRTVKQKDKEHH"
     gene            complement(7565..7738)
                     /locus_tag="ACHLCY_004276"
     CDS             complement(7565..7738)
                     /locus_tag="ACHLCY_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371398.1"
                     /translation="MKDQKATKPQVKFDTMKAFAGMGAAVEVLMKAAPNAFTHATVSG
                     KEQQGKLRRRKAA"
     gene            complement(7935..8468)
                     /locus_tag="ACHLCY_004277"
     CDS             complement(7935..8468)
                     /locus_tag="ACHLCY_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371399.1"
                     /translation="MLTLDEIGQSVRNNIQLIIDHVGLPLAVGPLSDDDYKILCGGYG
                     ELEWDYALSTYGNSREKYEFCIKLVQQGRVQGIPSGAAICVYGVEENIFRIHMIERFS
                     REDESHPLKGRMVLLTLMSAFIFCKAVECKVVHIVEPVPELVQYYESFGFRMEQCGYV
                     MSAVIDELQDIFLKFAQ"
     gene            complement(8615..8719)
                     /locus_tag="ACHLCY_004278"
                     /pseudo
     CDS             complement(8615..8719)
                     /locus_tag="ACHLCY_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
BASE COUNT         2580 a         2025 c         2084 g         2290 t
ORIGIN      
        1 gttcagcgac gctgaccacc agctgttgcg acaaacggtt atttgccgtt aaactgttca
       61 tgcgttagtt tctccacaga cacaaaacgc cacgacgccc ggagctgcac actcgcgggc
      121 gtcactcttt tctggagcgc aaaagatttt gtagaccagt gctgcatgct cctggagctt
      181 cgaaattgac agatacaact catcattaat tgctgtctgc tcgtgtggct ccactacccc
      241 atcttcgatt gccgaacgaa tctgctttga gtaactcccg atctgttcga tgacttccag
      301 caggcgctgg tttatatcgg cgttctctac ttcctcaagt tcaggaagcg atacaaacac
      361 cccaccagca gactgtgcga cagcatccgc aatgtagtga gtgccagccg cgcgctgtaa
      421 aatcattgcc catcccagcg ggaaaatctg atcgccatct gcacgaaggc ggttgaataa
      481 agcgttctct gttacatcca gccactcagc agcttcagcg taaccccccg gcaacgccgc
      541 gatagttttt ctgacagctt tcacgtacca ctcaggctgt ttttccactt tccagtgatg
      601 cttacccacg gcttatctcc tgttcctgtg gtttaaaccc attctggttt tggctagatt
      661 gaaaacgtgc cggataaaga atctgcattt cgctgatttc acccttaaaa aaattggcca
      721 gacgttctgc aagatcgata gatggaattt gttccagtct ttcaatacga ctcagcgtcg
      781 ctgtattgac ctgaacgccc gcagcaacat gctgcaaagt aaatccgtgc gccttacgca
      841 cattccgtaa tggtgattgc atataacctc cacatattgc gtgatgagca tattatttca
      901 cgcaaatatt ttgcgcaagt tgatttgctt aacgcgcaat aaagaaatgt aataaacgca
      961 tgaacatagg aaatcgagtc agacaacttc gccaggcgaa gaacatgaaa atcgccgatc
     1021 tcgctgaagc aataggagtg gatgcggcga atatctcgcg cctcgaaaca ggtaagcaga
     1081 aacaattcac tgaacaagcc ctgagtaata ttgccaggag cttaggtgtt gatattgctg
     1141 atctctttac ctcagacctc aaaagtaata ctgtatgtaa aaacagtatt agtgaggatg
     1201 ttgcgcaggt gaaggatgta ttccgtattg aaatgctgga tgtcagtgcc agtgcgggaa
     1261 atggccttat ccagggcggt gatgtcattg atgtgattca tgccattgaa tacagaactg
     1321 ataatgctgt atcgatgttt ggtggacgac cagcaaatca cattaaagtt atcaacgttc
     1381 gtggggacag tatgtgtcca accattgagc caggagatct catcttcgtt gatatcagta
     1441 tcaatcagtt tgatggggat ggtatatatg tatttggttt tgatgataaa atttacgtca
     1501 aacgactgca aatgatacct gataaactgc tggtaatttc tgataatcag atttaccgcg
     1561 aatggggaat taccagcgaa aacgaacatc ggtttatggt ctttggaaag gtcttaatca
     1621 gtcagtcaca aacccttaag cgacacaatt aaccccctac ctcaacatca attagccacc
     1681 agaaggtggc ttttcattac ccaccaaatt gcatatctcg caataaaaac acttgcatga
     1741 tgcgcaactt cattttatct ttctttccag acctacaaac aaggtactaa caaaatttgg
     1801 ttgtaacacg gcgtatggca catgcgtcgt tagcggtctg gggacgttaa aggggacaat
     1861 ccactccttg ctcgggcaaa caaaccaggt agccggaatg tgcaagtcaa tgaggatgct
     1921 gataagacgc ctaaccagcg tggcgattcg gtttgacgcc tgggaagaga ccagggtgca
     1981 acgatgaggg catttatgga accgcgacaa agtgtggtgc cgtaactggc taagtgctct
     2041 cagcgttgtg gtaatccgcg aaatggcgcg gcggtaagta tggcggggtt attccttccc
     2101 cgttgaggac accgggttgt caggttgacc atacgcttaa gtgacaagcc caccacaaca
     2161 gccactgctt tggcggtacc agtttgtaca cttgcttccg gctggtaccg ctctttttac
     2221 aaaacagaga agagcatcac cggacgacgg gctcataacc caatccaccc gggcggctgc
     2281 caccgcaggt gttcttctct gttttgtgga gaaaccaacc gaccttgcag ggtcgatatg
     2341 atgaggagca gcaaaatggc tagcgaacgc agtactgatg tgcaggcatt tatcggggag
     2401 ctggacggcg gcgtatttga aaccaaaatc ggcgcagttc tcagtgaagt cgcttccggt
     2461 gtgatgaaca cgaaaaccaa aggtaaggtc tcactcaacc tggaaatcga accatttgat
     2521 gagaaccgtg tgaaaatcaa acacaaactc tcatatgttc gcccgactaa ccgcgggaaa
     2581 atttccgaag aagacaccac cgaaacgccg atgtatgtca atcgcggtgg tcgcctgact
     2641 attctgcagg aagaccaggg acaattactg actcttgccg gtgaacctga cggaaaactc
     2701 cgcgcagcag gtcattaata tcgttcttaa ttaactgatt atttatctca tcactgaata
     2761 tctttatata gtgaggactt attatgtctc agaacttaga cgcaaccgca attaatcaaa
     2821 tccatgccct tatttctgct cagggtgtta atgaaattat cagtaagatt ggtgccgatg
     2881 ctgtggcatt gcctgagaat ttccgcattc atgatctgga aaaatttaat ttaaatcgct
     2941 tccgtttccg tggtgcgctt tccactgcca gcatcgatga ctttacccgt tattctaaag
     3001 atcttgcaga tgaaggcacc cgctgcttta ttgatgctga taatatgcgt gccgtcagtg
     3061 tgcttaacct gggtactatt gatgaaccag gtcacgcaga taacaccgcc actctcaaac
     3121 tgaaaaagac agcaccgttc tctgctctgt tgtctgttaa tggcgagcgt aactcccaga
     3181 aatcactgac agaatggatt gaagactggg ccgactacct tgtgggcttt gatgctaatg
     3241 gtgacaccat tcaggcaaca aaagcggctg cggcggtccg taaaatcacg attgaagcaa
     3301 accagaccgc tgattttgaa gataatgact tcagcggcaa acgctccctg atggagtctg
     3361 tcgaagcgaa gaccaaagac attatgccag tggcatttga atttaaatgc attccgtttg
     3421 aaggtctgaa agaacgtccg tttaaattac gcctcagcat tatcactggc gatcgtccgg
     3481 tactggttct gcgcattatt cagctggagg cggtgcagga agaaatggct aacgaatttc
     3541 gtgatctgct tgttgagaaa ttcaaggaca gcaaagtaga aacctttatt ggtactttca
     3601 ccgcctgatt tcattactgc aaatgcccct gagggggcat ttatggaaac gtaatttact
     3661 caataatcgc cggatggtga gggattcttt ttaccagaat tcagcgcggt gcagcgcata
     3721 tacgtggaga acaaaatgtc atttattaaa actttttccg ggaagcattt ttattatgac
     3781 aggataaata aagacgacat cgatattaac gatatcgcgg tttccctttc aaatatctgt
     3841 cgctttgccg gtcatctttc gcacttctac agcgtcgccc aacatgcggt tctttgcagc
     3901 cagctggtac cgcaggaatt tgcttttgaa gcattaatgc atgatgcaac agaagcgtat
     3961 tgccaggaca tccccgctcc actgaaacgc cttcttcctg actataaacg gatggaagaa
     4021 aaaattgacg ccgtaatccg tgagaaatac gggttacccc cagttatgag tacgcccgtg
     4081 aaatatgccg atcttatcat gctggcaacc gaacgccgcg atctcgggct tgatgatggc
     4141 tctttctggc ctgtactgga aggcatcccg gcaacagaga tgttcaacgt gattccactg
     4201 gcacctagcc atgcctacgg gatgtttatg gaacgtttta acgagttatc ggagttacac
     4261 aaatgcgcat gaatgttttc gaaatggaag ggtttcttcg cgggaaatgt gtaccgcgag
     4321 atctgaaagt gaatgaaaca aatgctgagt atctggtgcg taaattcgat gaagtacgtg
     4381 ctgaggctcg caacgagggt attaactata ccgcaagccg tcttgctgct gctttcaatc
     4441 acggatttat caataagcct ttggctgaag ttttcgacgt tacacgcatg attctgtcag
     4501 caaaagaaga gttagctaat gaatcgcatc cgattgatgg cctgtccggt gaatatgcag
     4561 agaaatccct tgaagaatgg gcggaacggc ttcgcaaagg aggcagccag tgactggaca
     4621 tgcagcaatc ctcgacatgt gctgtggcag tcgcatgttc tggttcgata agaatgacga
     4681 ccgggcgata tttagcgata tcagaaagga agagcacaca ttgtgtgatg gacgacgcct
     4741 gattatcagt cctgatctga tagctgattt tcgtgcacta ccatttgcag acgcatcttt
     4801 ttcgatggtt atattcgacc ctccgcatct tgagcgtgtt ggtgataacg cctggatggg
     4861 aaagaaatat ggacggctga ataaagatac ctggcgtgat gatttgcggc agggatttaa
     4921 agaagccttt cgtgtgttgc gtccatccgg cgttctgatt tttaaatgga atgaaacgca
     4981 aatacctgtt cgccagatat tggtactgac cgacagaaaa cctgttatcg gtcaacgaac
     5041 aggaaaaaac gataaaaccc actggattat ttttatgaaa taggcatcca gtgagtaggt
     5101 tcgtaaggtt acagatacgt atatctgaat aattaaattc agttctgtaa ataaaattta
     5161 atccttaacc ggagggattc ctgcaccctc agaacatcag gagaccgccc gaaagggcgg
     5221 taatgaaaaa tggctgaatt aaccaaatgg ctacaaaaca cgattaccgg aattgaaacg
     5281 gtagtagacg ataaatcgtt tgtatgtgat gaaatagtat tcaaaatcga tgtggttaaa
     5341 aacgtactta ccgcatttaa agtcgcgctg gtatcgctgg aagccgaacc ggtgacatgg
     5401 cgatatcgct acgtgagaaa aggcgttacg aactttcagg agaagccgtg ggttggtggc
     5461 tggaaatatg taccgacaaa agaggattgc aacgacaggc cgaactatga aattcaggcg
     5521 ttattcacgg cccagcctgt gccactgaca cccgaaggat tgattaaagc ggtgcgcttc
     5581 tatgaacagg ttaagagtga aaatccgcca gtcgaaaccg gagcatggaa agacgctgtt
     5641 gactgagtgc tcaaagaggc ttgtcaggct gtaaacattg gcatcaaagg agagtgagat
     5701 gaacggacaa atatcaattg ttcgaccggg agcatgtgac gatcgcgaga tacgaatgat
     5761 tattcgtctg gcgatgggga aaacaataac agctctcatt actcctgaaa atctcgcatt
     5821 agcattaaca ggaaagtcag acttgccagt agagctaaag ctgcgaaatg ttgagattaa
     5881 ggtgaaatag ttatgaatac tcttaccaaa gaatggttac agaacacgat taccagcatt
     5941 gagtcagcac gggatgaaat accgttcgga ctcgatgaag atcaaaacaa catgcttacc
     6001 gcattaaaaa ttacactggc atcactggca gcagtatcgg atgaacgagc agcctatgaa
     6061 ttatttatgg agaagcgttt cggggaatct gtagatcgcc gtagggcaaa aaatggcgat
     6121 agagaataca tggcatggga tatggcgctt ggctggatta tctggtgtca ccgcgccgcc
     6181 atgcttcagg gtagccaacc tgtaagccaa acttacaact tgccagaatt aatcgaaggc
     6241 atggaagttt ccattgatat aagcacttgt gatgctgatt taggtaatcg ctatttcggc
     6301 accgtcaccg aagcgttaga acttgataca gccaagaatg gttacatcct cttggttcag
     6361 gacgcagagc caaacttcga tgtaaatggc aactctccgg gaactccgga tagttggata
     6421 agctgtagtg atcgaatgcc tgaaaagggc cagaacgtgc ttatttcggt gaatttcgat
     6481 agctctctgg ttgaaccgct aatatgctcc gcacgctata ccggaagcac ctttcggcgc
     6541 ggagatgcaa cgattaagcc gggtaatggt attgagcaag caactcactg gatgccgcta
     6601 ccggaaccgc cgcaggaggt gaagtgatga acaacttaat gatcgacctt gagacgatgg
     6661 ggaaaaataa ggatgcaccg atcgtttcca ttggcgcggt gttcttcact ccagaaaccg
     6721 gagacatcgg acaagaattc tatgcggttg tcagcctaga cagtgctatg aagcaaggag
     6781 ctacacctga cggcgatacc atcctgtggt ggttgaaaca aagccctgaa gcgcgagctg
     6841 caatctgtat tgatgatact ttgtcgatca gcgatgctct ctcagaacta aaccatttca
     6901 ttaaccggca cgcagacaat acgaaatatt taaaagtctg gggtaacggg gccaccttcg
     6961 acaacgtaat tttacgtgga gcttacgagc gagcaggaca aatctgcccg tgggcgtact
     7021 ggaatgacca cgatgtacgc acgatcgtta cgcttgggcg ttccatcgga ttcgacccca
     7081 aaatggacat gcctttcgat ggcgaacggc acaacgccct ggctgatgcc cgtcatcagg
     7141 caaaatatgt ttccgctatc tggcagaaat taattcctgc caccagcaca gaattatgat
     7201 tttcccgggt gcagccggtt ttgatggaga aaattatgaa caccttgttt ttactgatgg
     7261 ctgaattcaa taccccaaac attgaactct cagcagttag ccaaaagtac tttggtatga
     7321 gtccagccac ggcagaagca aaagcaaacg cttgtaagtt gcccgttcca acatatcgca
     7381 tcggcacatc acaaaaagca aaacgttgca tcaatattca agatcttgcg gaatacatag
     7441 acaaaaggcg agaagaagga cgtatcgagt gggaacaggt cagaacagtc aaacagaagg
     7501 acaaagaaca tcactaaaga aaaaacccgc ctgaaggcgg gttttcaaaa agcaccagct
     7561 atgatcatgc tgctttgcga cgacgaagct taccctgctg ctctttacca gagacagtag
     7621 cgtgagtgaa cgcattagga gcagccttca tcagaacttc aacagcagca cccatacctg
     7681 cgaatgcttt cattgtgtcg aacttaacct gtggcttggt tgctttttga tctttcatag
     7741 aaaactcccg agacagtaaa ggcgtctcta accctttctt taaagctagc ttgtttcgct
     7801 aacttatgcc aatcgatcat gtcgattggt gacatcgttt cttagtagtt taagcacaaa
     7861 acgactgcca tagatgtacc tttaaggtaa tctggacggg tatcctacaa tttgtagacc
     7921 cttctcgtct atacctactg agcaaattta agaaagatat cctgcagctc atcaatgact
     7981 gccgacatca cataaccgca ctgttccatg cggaaaccaa aagactcgta atactgcacc
     8041 agttctggta ctggctctac aatgtggaca actttacatt caacagcttt acaaaatata
     8101 aaagcactca taagagtgag taaaaccatg cgccctttca atgggtgaga ttcatcttct
     8161 ctagaaaacc tttcgatcat atggatacga aagatgtttt cttcaacccc ataaacacaa
     8221 attgctgctc ctgatggtat tccctgaacc cgaccttgct gaacaagttt tatgcagaac
     8281 tcatactttt ctctggagtt gccataggta cttaacgcat agtcccattc aagctcacca
     8341 tagccaccac acagaatctt gtaatcatca tcactgagcg gaccaacagc aagaggtaag
     8401 ccgacatgat caataatcaa ctggatattg ttacgtactg attgacctat ctcgtccagg
     8461 gtaagcatca tggcctctca agcggaacac taaaagtcgc attatatctc attcttaagc
     8521 cgcgtatgga ttacaccttg aaatgaaaac gccgggttcc caataggctc ccacaaagta
     8581 tataactatt tgtttttcaa aaacggtaca tcctatcgag cattggtgca acgctaaacc
     8641 gaccactcca gtgaacgtca gttttttcag gcattgcgct ggtttggttg attttttgca
     8701 tttcagaatt accgtgcatt ttcaaatgta gagattattt tatcgatata tcatggggtt
     8761 atgttattca gcatcactat tcaggaggct caatagcggg gtactatacc ataacaacag
     8821 gaagcgcctg tctcattgca aaagaaaatt gagatcctct caaggcatga agctctcacg
     8881 aagtgatgga aataatctta ttagccgtta actttgttaa ggccaatgat aaacaatcca
     8941 ggttcgacga taaataaaaa atcacacatt aaactctgg
//
LOCUS       DAZGTR010000154         8908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGTR010000154 DAZGTR010000000
VERSION     DAZGTR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.156"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(220..1320)
                     /gene="chaA"
                     /locus_tag="ACHLCY_004279"
     CDS             complement(220..1320)
                     /gene="chaA"
                     /locus_tag="ACHLCY_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP0371400.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1590..1820
                     /gene="chaB"
                     /locus_tag="ACHLCY_004280"
     CDS             1590..1820
                     /gene="chaB"
                     /locus_tag="ACHLCY_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP0371401.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1978..2673
                     /gene="chaC"
                     /locus_tag="ACHLCY_004281"
     CDS             1978..2673
                     /gene="chaC"
                     /locus_tag="ACHLCY_004281"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP0371402.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2717..3070)
                     /gene="ychN"
                     /locus_tag="ACHLCY_004282"
     CDS             complement(2717..3070)
                     /gene="ychN"
                     /locus_tag="ACHLCY_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP0371403.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3255..4649
                     /gene="ychO"
                     /locus_tag="ACHLCY_004283"
                     /gene_synonym="iatA"
     CDS             3255..4649
                     /gene="ychO"
                     /locus_tag="ACHLCY_004283"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP0371404.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4650..5300)
                     /gene="narL"
                     /locus_tag="ACHLCY_004284"
     CDS             complement(4650..5300)
                     /gene="narL"
                     /locus_tag="ACHLCY_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP0371405.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5293..7088)
                     /gene="narX"
                     /locus_tag="ACHLCY_004285"
                     /pseudo
     CDS             complement(5293..7088)
                     /gene="narX"
                     /locus_tag="ACHLCY_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
     gene            7427..8818
                     /gene="narK"
                     /locus_tag="ACHLCY_004286"
     CDS             7427..8818
                     /gene="narK"
                     /locus_tag="ACHLCY_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP0371406.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2172 a         2171 c         2301 g         2264 t
ORIGIN      
        1 tgtggatact acgtcgcaat tcatcttcac ttcatccctg aaatgtttgc aataaagagt
       61 acattccggc ttttcaacag ctgttgcagt ggtttcgtgc aggttgtggt acaggctcgc
      121 aattctgatt aacgagctgt ccaggtagta tgaagaaata caataagcag gaatttatct
      181 tatctccccc gatttccgtc atcggaggag atataactgt caggcaaata tcgtcatcaa
      241 ataggcggca aacagtgcca gatgcgctgc gccattgagc acgttagtac gtccggtgga
      301 gaaggagata tggcacagca ctaaagaggc caccatcacc accatttctg gcgcaccaag
      361 tgcaaactgt aattcgttac ccgtcataaa ggcaattagc gtgacgacag gtacggtaag
      421 cgaaatggtt gctaacactg aaccaaagaa cagattcatc gcgcgctgga cctggttgtt
      481 caacactgct tttaacgcac ctaaaccttc cggcgacaga atcaacagag ccaccaggaa
      541 gccagtaaag gcgacagggg cattcatgct gtcgagcaat gtctccagcg ggctggcatt
      601 cattttggtg actgcaataa cggcaatcag atggataatc aaccagatag catgccacac
      661 gctgctatgg gcagacggtt taccgtgatg cgggtcatcg tcatcactgt catcttcgtg
      721 ctcgtagaca aacaaacttt gatgcgtttt ggtctggatc agcaaaaata cgccatacat
      781 tgccgcagaa attaatgcta ccagtaacgc ctgaccggtt gaaaaattcg ccgcaggcag
      841 agccattgga aataccagta cgattatcgc cagggggaac agcgcaatta aatactgctt
      901 gataccaaac agattcatat attgggtggc aaacttacga ccgcccaaca ataatgaaaa
      961 gccaaccagc ccaccggtaa caatcataat gattgaatag agcgtatcac gcattagcgt
     1021 tggcgcggcg tcgccggttg ccattaaagc tgaaatcaaa ctgacttcaa gaataaccac
     1081 tgaaaggcta agaataagcg aaccgtaagg ttctcccagg cgatgggcta atacgtccgc
     1141 atgacggaca acactaaagg cgctacttaa aataccaata agcgcaagaa gattgatggc
     1201 aatgaccact ggtagtgtct ggctgcttcc ccacaggaac agcactacca gcgccagaac
     1261 cgggaaaata agcgaagtct ccttgtggcg ggtttttacc gcctcttgag catttgacat
     1321 tatggttatc cctttgcaga tgaatttatc ggaaatgtaa aaaataggta ggaaaaataa
     1381 cagaaagtgt ctggatatcg gtaacatttt acgaattttt accccactgt cgatttttta
     1441 ctcattggga cataaaaata agtattacgt ttagacaatg tttgttttag cgtttcttaa
     1501 gaacagtctg acatgaaaat tcttatgttt tggcaagagt agatattgtt gaccacactt
     1561 aatgttcaac tttgtaaaag gagtcaacga tgccgtataa aacgaaaagc gatctgccgg
     1621 aaagcgtaaa gcacgttcta ccgtctcatg cccaggatat ctataaagaa gcgttcaaca
     1681 gcgcatggga tcaatataaa gataaagacg atcggcgtga tgacgccagt cgcgaagaaa
     1741 ccgcgcataa agtggcctgg gctgctgtga agcatgaata tgccaaaggg gatgatgata
     1801 aatggcataa aaaatcgtaa aaccggtagc ttagttaaag ctattcgtgc ggtgttgcct
     1861 tgcaagtggt ccgtggattg catattgtcc cgttagtggt ttcaaaatga gcagtaaaaa
     1921 tgtccggaag acaccaaaaa gttgtcgcag ggaagtatgc agtggcggag gtgtaaggtg
     1981 ataacgcgtg atttcttgat gaatgccgat tgtaaaacgg catttggtgc cattgaagaa
     2041 tcactcttat ggtcagcaga gcaacgggcg gcttcgctgg cggcgacgct ggcttgtcga
     2101 cctgatgagg gaccggtgtg gatcttcggt tatggatcgt tgatgtggaa tccggcactg
     2161 gagtttaccg agtcgtgcac cggtacactg gttggatggc atcgcgcgtt ttgcctgcgc
     2221 ctgaccgccg ggcgcgggac tgcgcaccag ccgggacgga tgcttgcact gaaagagggc
     2281 ggacgcacta caggcgtcgc ctatcgactg ccagaagaga cgctggagca ggaactaacc
     2341 ctgttgtgga agcgcgagat gattaccggc tgttatctgc caacctggtg tcagcttgat
     2401 cttgatgatg gatgcacagt aaacgccatt gtgtttatta tggacccgcg acatccagaa
     2461 tatgaatctg acactcgcgc tcaggtcatc gcgccgttga ttgcggcggc gagcggtccg
     2521 ctgggaacca acgcacaata cctgttttca ctggaacagg agcttatcaa actgggaatg
     2581 caggacgatg ggctgaatga tttgctggta tcggtaaaaa aactgctggc ggagaattat
     2641 ccggatggtg tgttacggcc gggattcgcc tgagtaaact tccctcatag tggggcgtca
     2701 gacgccccct caaacattaa aatgtgagca ctttatcggc tgacagcgtc cattgcgcca
     2761 gttccacaag agtaccgatt tccaccccat caatcagagg aagtgtacta atcccgcgcc
     2821 cgtcggtaca ggttttgcac aatttcaccg gtacattctg agcggtaagg atctccagca
     2881 tttgctgaat gttgtagcct tcccctggtt tttgcccgcg caacccggct gtgaccgcat
     2941 cagacatcag gaacagacgc agatccagat tgctctcctg ctctcgtaac gcaatggcta
     3001 gccgcaagct gttaaacaag gattcgctcc cgtaaggtgc gccattggca acgatcacga
     3061 ttttttgcat tatttactcc tgtattcagg gaattagaca ttcatcttct atcttactgc
     3121 ttctgcagcg tctgaccaat cggtcacatt tttaaggatt ttcctgaaag cgcgagaaaa
     3181 tacgacaaaa gttgccagta atcgttattc tttaaggcta tggtttttca ttattaccgg
     3241 aagttaccga cgttttgagc cgtttcgttc ctcgcattat cccgttttat ttactcttgc
     3301 ttgtagcagg cggtacagct aacgcacaat ctaccttcga gcaaaaagcg gcaaatccct
     3361 ttgataataa caatgatggt ctgccggatt taggcatggc tcccgaaaat catgatgggg
     3421 aaaaacactt tgctgaaatt gtgaaagatt tcggcgaaac cagtatgaat gataacgggc
     3481 tggatactgg cgagcaggca aaagctttcg cattgggaaa agtccgcgac gcgcttagtc
     3541 aacaggttaa tcagcacgta gagtcctggc tatcaccgtg gggaaatgcc agtgttgacg
     3601 tcaaagtgga taacgaagga catttcaccg gcagtcgtgg aagctggttt gtgccgttac
     3661 aagataatga tcgttatctc acctggagcc agcttggtct tactcagcag gatgatgggt
     3721 tggtgagcaa tgtgggcgtt gggcaacgct gggcgcgcgg caactggctg gtgggttata
     3781 acacttttta tgacaacttg ctggacgaaa atcttcagcg agcgggcttt ggtgccgaag
     3841 cgtggggcga atatttgcga ctatcggcaa acttttatca gccgtttgct gcatggcatg
     3901 aacagacagc cacgcaggaa caacggatgg cgcgcgggta tgacctgaca gcccggatgc
     3961 gcatgccgtt ctatcaacac ctcaatacca gtgtcagcgt agaacagtat tttggtgatc
     4021 gtgttgattt gtttaactct ggtacgggtt atcacaatcc cgtcgcgttg agtctgggat
     4081 taaattacac ccctgtgcca ttagtcactg tgacggccca gcataaacag ggtgaaagtg
     4141 gcgagaatca aaataacctc gggctgaatc ttaattaccg ctttggtgta ccgctcaaaa
     4201 aacaactttc tgcgggtgag gttgccgaaa gtcagtcgtt acgtggtagt cgctatgaca
     4261 atccgcagcg aaataatcta ccgactcttg agtaccgaca gcgaaaaacg ttaacggtgt
     4321 ttctggcgac accgccgtgg gatctaaaac ctggcgaaac agtgccgctg aaattacaaa
     4381 tccgcagtcg ttacggtatt cggcaactga tttggcaggg cgatacgcag atattaagtt
     4441 tgacgccggg cgcacaagcc aacagcgcgg agggctggac gctgatcatg cctgactggc
     4501 agaacgggga aggggcaagc aatcactggc gattgtcggt ggtggtggaa gataaccagg
     4561 ggcagcgtgt ctcctccaat gagatcacgc taacgcttgt cgaaccgttc gatgcattgt
     4621 caaacgacga actgcgctgg gaaccgtaat cagaaaatgc gctcctgatg cacccatacc
     4681 gctgcttcca cgcgagactt gagcttcatt ttcttcagca tgtgcttgac gtgcactttt
     4741 actgtgcttt cggtgatatc caggcggcgg gcaatcatct tgttcggcaa accctgggca
     4801 atcagcttga gaatatcgcg ctcgcgtggg gttaactggt taacatcgcg ctcagtagtg
     4861 gcacggttag cgcgcaagct ggcggccaga acaggcgtta atgcttcgct taataccatt
     4921 tcgccagcag ctgcctgatg caatgctttc agcagatctt ccggttccat atcttttaac
     4981 agataaccat ccgcgccgcg tttcagtgcg gtgaccacat cttcttcatg gttagagacg
     5041 ctgaatacca caatgcgccc tgagagggac ttttcgcgca gtttatccag cgtttccaga
     5101 ccgttcatgc cgggcatatt gagatctaac aggatcagat cgggatcaag agactccgcc
     5161 agttcaatac cctgttcgcc attactcgct tcgccaacca cggtgatatc tggtgccata
     5221 ctgataagct gttttacgcc agttcgcagc atcgggtgat cgtcaatcag caggatagta
     5281 gccggttcct gattactcat gggtatctcc ttggacgtct gtgaaagtat ttcgggaata
     5341 aaggtgacca ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgcctcgt
     5401 aaactttgcg cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt
     5461 tcaggcacgc cgcagccgtt atcctggacg gtcagtttga cctgattatc gttttgcgcc
     5521 accgtcacca cgacttcact cgcttgcgag tgtttgaggg cgttacttaa tgcctcacgg
     5581 gcaatttgca acaagtggat tgcctgatgc gaaggcacca ggcgaggcgg caattgataa
     5641 tccagcttca ccgggaagcc aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc
     5701 ggacgtaatc caggctcggt gagctgcaag cggaatgtgg tgagcaattc acgcaactgc
     5761 gcccaggatg cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc
     5821 agcgcatcgc cctgcatctg taaacaactc acctgcatct tcatgcaaga gagagattgg
     5881 gcaatagaat catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt
     5941 tgctgacgtt cctgatggcg atccagcgcc agcgtggcgg tgagttgttc aaccagggta
     6001 tccaccagtt gttgttgatc atggctaaga tgacgcccct gcggcagggt cgccagcaaa
     6061 ataccgtact gcgtatgaga gtcagccagc cgccacttca gggttgtgcc gcgatcacca
     6121 acgggtaata cgccgcgcgg gcagagctgg cagcctttat catcacaagt catatctggt
     6181 tggcaggtaa actcctgatg attctcttca tcatcagtat cataaacccg cagttcgata
     6241 tcacgtagca gggttaaatt ctgtaagccg ttgagtacag gtgacaggcg ttcacacagc
     6301 ggggcttggg aatgcaaacg gcggttagcc tgccataaaa aagagaggat ctgattttta
     6361 tgctccagcc cggcggtttt ctcctgaacc cgctgctcaa gtacggcata actttcggcc
     6421 agttctgcag acatattgtt caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg
     6481 atgtttgcgc gttgggtaaa atcgcgatga ctgacggcac tcgccattgc cagcagttgc
     6541 cgccacggtt gtagcagtcg cgcccgcaac cagataatag tgaacaccag taaaagtgcc
     6601 ataaataccg ccattacccg atggaccagt accactgtct cgatgcgcat ttccgtggtg
     6661 cggtcaaaac cagataccag ttgatcaagc ccggcaacaa actggctgac atccgctgac
     6721 accgtttctc ggttttgtgc acgcatcaac gcagggatca gttcattacg ccagtaatct
     6781 tgtaaaccct gtaattgcgc cagttgtccg tctcgttctg ctgctcgagt caactcggcg
     6841 ctaaatgccg tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact
     6901 gctgccaaca gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg
     6961 ctgccctgaa cgccttgcac cagccagcca gaaaccgcca tccctgccag tccaatagca
     7021 gtagaaagca acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt
     7081 ttaagcatgt aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac
     7141 gatacattct ggaatggcag tattctcggc tattggctga agtataccca tacctggaaa
     7201 gagttactcc ttatttgccg tgtggttagt cgctttacat cggtaagggt agggattcta
     7261 cagcaccgtg aaaaatctca taatttttat gaaatcactg tactcactat gggtaatgat
     7321 aaatatcaat gatagataaa gttatcttat cgtttgattt acatcaaatt gcctttagct
     7381 acagacacta aggtggcaga catcgaaacg agtatcagag gtgtctatga gtcactcatc
     7441 cgcccccgaa agggctactg gagctgtcat tacagattgg cgaccggaag atcctgcgtt
     7501 ctggcaacaa cgcggtcaac gtattgccag ccgcaacctg tggatttccg ttccctgtct
     7561 gctgctggcg ttttgcgtat ggatgttgtt cagcgctgtt gcggtgaacc taccgaaagt
     7621 cggctttaat tttacgaccg atcagctatt tatgttgact gcgctgcctt cggtttctgg
     7681 cgcgttatta cgtgttccat actcctttat ggttcctatc ttcggtggtc gtcgctggac
     7741 ggcgttcagc accggtattc tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga
     7801 tacctccacg ccttatagcg tcttcatcat catctctctg ctatgcggct ttgctggcgc
     7861 gaacttcgcc tccagtatgg caaacatcag cttcttcttt ccgaaacaga agcagggtgg
     7921 cgcgctgggt ctgaatggtg gtctgggcaa catgggcgtc agcgtcatgc agttggttgc
     7981 tccgctggtg gtatcactgt cgattttcgc agtatttggt agccagggcg tcaaacagcc
     8041 ggatgggact gagctgtatc tggcgaatgc gtcctggata tgggtgccgt tccttgccat
     8101 cttcactatt gcggcgtggt ttggcatgaa cgatcttgct acctcgaaag cctccatcaa
     8161 ggagcagttg ccggtactca aacggggtca tctgtggatt atgagcctgc tgtatctggc
     8221 aaccttcggc tccttcatcg gcttctccgc gggctttgca atgctgtcaa aaacgcagtt
     8281 cccggatgtt cagattctgc aatacgcttt cttcgggccg tttattggtg cgctggcgcg
     8341 ttctgcaggt ggtgcattat ctgaccgtct gggcggaact cgtgtcacgc tggtgaactt
     8401 tattctgatg gcgattttca gcggcctgct gttcctgacc ttaccgactg acgggcaggg
     8461 cggaagcttc atggcgttct tcgcggtctt cctggcgctg ttcctgacag ctgggctggg
     8521 tagtggttcc actttccaga tgatttcagt gatcttccgt aaactgacaa tggatcgcgt
     8581 gaaagcagaa gggggttctg acgaacgtgc gatgcgtgaa gcggcaaccg acacggcagc
     8641 ggcgctgggt ttcatctctg cgattggcgc tattggtggc ttctttatcc cgaaagcgtt
     8701 tggtagctcg ctggcattaa cgggttcgcc agtcggcgca atgaaggtat ttttgatttt
     8761 ctatatcgcc tgcgtagtga ttacctgggc ggtatatggt cggcattcta aaaaataaac
     8821 cgttactcgt catacttcgg gttacatgtg ctgcggctgc gttcattcac cccagtcact
     8881 tactttagta agctcctggg attcattc
//
LOCUS       DAZGTR010000155         8816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGTR010000155 DAZGTR010000000
VERSION     DAZGTR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.157"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            103..933
                     /gene="yjiC"
                     /locus_tag="ACHLCY_004287"
     CDS             103..933
                     /gene="yjiC"
                     /locus_tag="ACHLCY_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0371407.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELIRKYERARLDTENIY
                     LLPLVRGNNHNYDGKSVVEIRKLDISKEYWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1074..1847)
                     /gene="uxuR"
                     /locus_tag="ACHLCY_004288"
     CDS             complement(1074..1847)
                     /gene="uxuR"
                     /locus_tag="ACHLCY_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP0371408.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2062..3522)
                     /gene="uxuB"
                     /locus_tag="ACHLCY_004289"
     CDS             complement(2062..3522)
                     /gene="uxuB"
                     /locus_tag="ACHLCY_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP0371409.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3603..4787)
                     /gene="uxuA"
                     /locus_tag="ACHLCY_004290"
     CDS             complement(3603..4787)
                     /gene="uxuA"
                     /locus_tag="ACHLCY_004290"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP0371410.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5127..6470
                     /gene="gntP"
                     /locus_tag="ACHLCY_004291"
     CDS             5127..6470
                     /gene="gntP"
                     /locus_tag="ACHLCY_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP0371411.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISVVA"
     gene            complement(6720..7622)
                     /gene="fimH"
                     /locus_tag="ACHLCY_004292"
     CDS             complement(6720..7622)
                     /gene="fimH"
                     /locus_tag="ACHLCY_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0371412.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7642..8085)
                     /locus_tag="ACHLCY_004293"
     CDS             complement(7642..8085)
                     /locus_tag="ACHLCY_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0371413.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8158..8688)
                     /gene="fimF"
                     /locus_tag="ACHLCY_004294"
     CDS             complement(8158..8688)
                     /gene="fimF"
                     /locus_tag="ACHLCY_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP0371414.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
BASE COUNT         2096 a         2279 c         2225 g         2216 t
ORIGIN      
        1 tacaacagaa acatgcatgt catttgttta cttctttttt actacagaaa aatttatcta
       61 tttttctaaa catcagtgaa atactcataa aaggtaacta ttatgtctat gccattaagc
      121 aatgcccttc agagtcaaat tattacagat aatcacttcc ttcatcatcc aaaagtcgac
      181 agtgagttaa tacgtaaata tgaacgtgca cgcctggata cggaaaatat ctatcttctg
      241 cctcttgttc gtggcaataa ccataattac gatggaaaaa gcgttgtcga aattagaaag
      301 cttgatatta gtaaagagta ctggccattt aattatgtga cagaagcatg cagagaatcc
      361 gatggtatta caacaacagg acgtatgctc tatcgaaact tgaaaatcac ctcagcttta
      421 gacgaaatat atggtggtat atgtaaaaag gcgcatgctg ctacagaatt agcagaaggt
      481 ttacggctaa atttatttat gaagagccct ttcgatcctg ttgaggacta tacagttcat
      541 gaaataacat taggccctgg ttgcaatgtt ccaggttacg ccggaaccac gataggatat
      601 atttcaaccc ttccagtttc tcaggcaaaa agatggacca atgagcagcc aagaattgat
      661 atctatattg atcagataat aactgtctca ggggtagcaa attcttccgg attcgccctt
      721 gccgcattac ttaatgctaa tattggaatg ggaaatgacc caataattgg tatagaagcc
      781 tatcccggta ccgcggagat ccatgcaaaa atgggatata acgttatccc cggtgatgaa
      841 gatgcgccac tcaaacgcat gactctgcaa ccgtcatctc ttccagaact gtttgaatta
      901 aaaaatggtg aatggaatta tattggtaaa tagccactga gaagaatgag tactaatatt
      961 caggttatac agatatttaa acaataccct gcaatttaca aagtaatact cattgcgact
     1021 tcttaattta agaaagcgca aggaacgttt acccttgcgc ttattataat aagtcaggcg
     1081 tcgactttat ccagcggcca tgaatcaaac agatagccgt caaaataaat atcgtcaacg
     1141 ttcgagaatt ccagcagacg ttgcttaacg ttttccagat gctgccacat tgccagcttc
     1201 gccgctcggg catctttttt gattaacgcg gcgaggatct gtttgtgatc gcccaaccac
     1261 tctttgcgat acaggctgtc atccagatgg ctgtgcaact ggatccacat tgggttgttt
     1321 tcccgccact gccaggactg acggaacagc tccaccagca tgctgttatg cgttgcttcg
     1381 gcaatagcga gatggaactg catgtcaccg ctttcgctgc tgcctggcgc actggaagcc
     1441 agttcacgct cttccagttg caatgcctga cgcattttga cgatatcttc gcgggtagcc
     1501 tgcaaagcgg caaactcggc gatgttgctc tccaataact gccgcgcctg taacagctca
     1561 aaaggaccgg catcgttgca gacgttggca tccggactgt ctgtgttctg gctgcctgag
     1621 ttatcaagaa catagatacc ggcaccccgg cgtacttcca ccagcccttt gatctccagc
     1681 atgatcagcg cttcacgtac caccgtccgc gtgacatcaa gcatttctgc aatttcacgc
     1741 tccggcggca gccgttcgcc agggttgtac ggcgtcttta tgatcagatc gcggatcatc
     1801 gccccgactt cctggtaagg tctttgcgca gaggtggctg atttcatggt gttgtgtccg
     1861 ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga acgccttatc cggcctacaa
     1921 cgggcaattt tacggccccc gttaatctca aactatacca ttagcactgg aagttggcac
     1981 gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata tcagtttgat tgaccagtaa
     2041 gtctgtaggg taatcagtta gttacagcgc agccacacac tcgcgcgcac cgcgttcgca
     2101 cagctgctga tatgccgccg tcactgcgcc aacaaagtcg gcattctgcg gcagatcatc
     2161 ggcaaaaata ccgctcaggc ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc
     2221 gttgatcttc tggaactccg ccagcatcgg gtcgaccacg tcaatggcat taccctgctc
     2281 atccacgccc tgggtgtaac gcatccagcc agccacgccc agcgccaggt gacgccagct
     2341 gccgccgttt tgcaggtgca gacgcaccgg gtccagcaga cgctgcggta acttctggct
     2401 gccgtccatc gcaatctgcc aggtacggtg acgcagagac gggttgctga aacgctcgat
     2461 cagcagcgtc gcataggcgt tcaggtccgt accttccggc atcgacagcg ttggcgcttg
     2521 ttcctgcatc atcagggaga aggccgcttt gcgataatcc gggttagtca tggtgtcggc
     2581 aatagtttca tagccgccga ggtaaccgag gtatgccagg aaagagtggc tgccgttcag
     2641 catacgcagc ttcatcattt cgaacggcac aacgtctgca acgaactgtg cgcccacttt
     2701 atcccagtcc ggacgaccat taacgaagtt atcttcaatc acccactgac ggaacggttc
     2761 gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca gcaatttcct gtaaggtttc
     2821 tggcgtcgcc gccggaacga tgcggtcaac cattgtgcac gggaaggtga cgttctcttc
     2881 aatccatgcc gctagctgcg ggtcacgcgc ctgagccagc cccagtaccg cgaccttcgc
     2941 cacatgaccg ttttcacgca cgttatcgca ggacatcacc gtaaacgctt tcaacccttt
     3001 ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg
     3061 gttttctaga tcgtgcttga tcagcgggtt attgagatcc agttgaccgc ttgccgcatc
     3121 agcgcagtag cctttttccg tgaccgttag agagacaatc gccgtttgcg gacgcgccat
     3181 cgcgttgaga ataccttcgc agccgtcgat ttccggatgc agcgcttctt tcatcgaacc
     3241 gataattttc agctcggtgc tttctgcgcc tttttccgct acggtgtaca gcagttgctg
     3301 ttttttcagg ttttcgatca gcacgcggtc gttgcctggc atcaggttaa cttcgcagat
     3361 gccccagtcg ctgtcggtgc tttccagcag atgatgggta tacagcgcct ggtgcgcgcg
     3421 gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc agacgagaat gatcccatga
     3481 cgggcgggca accggcagat tgctgtcaac aatagtagtc atcgtaaact ccagaatccg
     3541 tcttcagaga gtgacggcaa ggaagaagga accggcacgg cgcgcagcca tgccggtgga
     3601 tattaacggc taaagaaagc gcgctggatc gccagttcga caccgcgaac ttcggccagg
     3661 cctttcagac gaccaattgc ggagtaacct gggttggttt tcttcttcag gtcgtccagc
     3721 atctgatgac cgtggtccgg acgcatcggg atcaggtctt ctttgccttc cgctttacga
     3781 cggtgttctt cttcaacaat cgctttcacc acttcgtaca tatcaacgtc accgttcagg
     3841 tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca tggtggagcg cagatgggtg
     3901 aagtaaatac gcggaccgaa ctgcttgatc atatcaacca gatcgttgtc agcacgcacg
     3961 ccgtaggaac cggtgcacat ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc
     4021 cactgcatat cttcaatggt ggaaacaatg cgcggcaggc cgaggatcgg gcgcggcgga
     4081 tcgtccgggt gaaccgccat acgcacgcca acttcttcag caactggaat aatcgctttc
     4141 aggaagacgg caaagttttc gcgcagcttc gctttgtcga tatctttgta cagctccagg
     4201 tgtttacgga actggtcgag ggtgtagcct tcttccgcgc ccggaagacc agcaatgatg
     4261 ttacgggtca gacgcgcttt gtcttcgtcg ctcatagtgg cgaagcgaac agcagcctga
     4321 gcaatttctt cttcggtgta atccgcttcc gcgcctggac gtttcaggat atgcatttcg
     4381 aatgcagcga attcgatctg gtcgaagcgc agcgctttgg agccgtctgg cagcacgtat
     4441 tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg
     4501 ccgcactgcg ccaggttgcg cagagtctgc tgatagttag cgatccactg ctcatagttg
     4561 ccagtgtggg ttttgatatc ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg
     4621 cctgcgtctt caacgatcgc cttgcgtttg aggatctctt ctacggacca tacttcgccg
     4681 ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct
     4741 aaagaaaccg gatcgtttgg gccgtaccag cgccaggtct gttccatgat ttttcctctt
     4801 tcacatagtg ggttccacag caaagagacg cggaaccctg gagttgttta ggtatccgga
     4861 tgaacaacca gaccgcagcc gtggcgttgc atcggatgag atggcgtata agttctacct
     4921 tttggtaaga catgtcaacc cgaattctga aattggttaa ccacaccaca agaatttcac
     4981 tctcctggtt gttgctgcaa attgcagcaa aaggttatcg atggttattg atcagaggat
     5041 gtgacattca tcgcaacaat ggttgaccaa tttacataac atgtcgccca aaataacaac
     5101 ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa cattctctgg gtagtattcg
     5161 gtatcggcct gatgctggta ctgaatttga agttcaaaat caattcaatg gtggctttgt
     5221 tggtggcggc gctgtccgtc gggatgctgg cgggcatgga tttgatgtcg ctgctgcaca
     5281 ctatgaaagc gggcttcggc aacacgctgg gggaactggc tatcatcgtg gtgttcggtg
     5341 cggtcatcgg taaattgatg gtcgactccg gcgcggctca ccagatagcg catacgctgc
     5401 tggcgcgtct cggtctgcgc tatgtacagc tgtcggtgat tatcatcggc ttgattttcg
     5461 gtctggcgat gttctatgaa gtggccttta tcatgttagc gccgctggtt attgttattg
     5521 ccgccgaagc taaaattccg ttcctgaaac tggcgatccc ggcagtagca gctgccacta
     5581 ccgcacattc actgttccca ccgcagccgg gtccggtggc gctggtgaat gcttatggcg
     5641 cggatatggg gatggtttat atctatggcg tactggtgac gatcccaagt gtaatctgcg
     5701 caggtctgat cctgccgaag ttcctcggca atcttgagcg cccaacgcca tcattcctga
     5761 aagcagatca accggtagat atgaataatc tgccctcttt cggcgtttcg attctggtgc
     5821 cgctgatccc ggcgatcatt atgatctcta ccaccatcgc caatatctgg ctggtaaaag
     5881 atacccctgc ctgggaagtg gttaacttta tcggttcctc gccgattgca atgtttattg
     5941 cgatggtggt tgcattcgta ctctttggca ccgcacgtgg tcatgacatg cagtgggtga
     6001 tgaacgcttt tgaaagcgcg gtgaagagta ttgcaatggt gattctgatc atcggtgcgg
     6061 gtggcgtgct gaagcagacc atcatcgaca ccggcattgg cgacaccatc ggcatgttga
     6121 tgtcccacgg caatatctcg ccctacatca tggcatggct gatcactgtg ctaattcgtc
     6181 tggcgacggg tcagggtgtc gtttcggcga tgaccgccgc cgggattatc agtgctgcaa
     6241 tcctagatcc agcaactggt cagctggttg gcgtgaatcc ggcgctgctg gtgctggcga
     6301 cggctgcggg ttccaacacc ctcacccaca ttaacgatgc atctttctgg ttgttcaaag
     6361 gttactttga cctgtcagta aaagacacgt tgaaaacctg gggtctgctg gagctggtca
     6421 actccgtggt tgggctgatt attgtgctga ttattagcgt ggtagcgtaa aagaacacca
     6481 gagcctgcca ttggcaggct ctgactgatg acaaagtcta aaaatgtgcc cggccagtcc
     6541 cctcgcccct ccggggagag ggttagggtg aggggaacag accagcaaag tgcgctttgt
     6601 ttatgccaga tgcgacgttg acgcgtctta tctggcctac aaagggctaa cgtgcaggtt
     6661 ttgtaggtct gatagcgtag cgcctcaggt accagcatta gcaatgccct gtgatttctt
     6721 tattgataaa caaaagtcac gccaataatc gattgcacat tccctgcagt cacctgccct
     6781 ccggtacgtg cataatttgc cgttaatccc agacttaccg ccgaagtccc tactgctcct
     6841 aacgataccg tgttattcgc tggaataatc gtaccgttgc gcgtcaactg tacgccgacg
     6901 ccctgtgcag gtgaaaacga cgcggtattg gtgaaaatcg agttgcccgc atctgcggtt
     6961 gtgccggaga ggtaataccc caggttttgg cttttcgcac aataaacggt aagaggaatc
     7021 ggcactgaac cagggtagtc cggcagagta acggtgacat cacgagcaga aacatcacag
     7081 ccgccagtag gcaccaccac atcattattg gcgtaaatat tccacacaaa ctggaaatca
     7141 tcgctgttat agttgttggt ctgtcgcaaa ataagcacgg caattaatga gccagcttta
     7201 attgccaccc cgcccgcact gctcacaggc gtcaaataaa gcgccaccgg ccacggctta
     7261 tccgttctcg aattataaac aacgcgcggc gtttcgctgg tggtcggaaa tggatagcta
     7321 ctgccactat attttacggt cccggaaaaa ttagataaca cgccgccata agccgagcct
     7381 cgttgcagtg tgacatagtc tgtaatggtt tccggataat cgttatggca aaagatttgc
     7441 gtcgaaagat ctacgaccag gttttgcccc acattcacgg caggcgcaag gtttacataa
     7501 acattagcgc tgccaccgcc aatagggata gctgtaccat tggcggtttt acaggcgaat
     7561 gaccaggcat ttaccgacca gcccatcagc agtacagcaa acagggtaat aactcgtttc
     7621 attacaatca tctcttcggg ttcagctgta ggtataggtg atgctaatca ctgcctgaat
     7681 ggttccctga gtggctccgc catttactgt caatgctctg acctgtaacg ggaagtgcgc
     7741 tgattgtgag gaatcatcca cctgaactgt tttggttgcg ccagtattca atgtgttgcc
     7801 actgtcatcc tgtagctcta actggatgtt ttgcgcggtc ccctggtttt tataatatcc
     7861 ggtactgtcg gctgccccgc tgaagctggc agtgaccctc gacgttccca ccggacaatt
     7921 agtcaactca agcgcaacat catgccaggc cgatgccgcc ccggcagaca tcagactgaa
     7981 agaataaaga tcgccgagat caaccgtggc attggtggtg gaaactgtgc acggtttggc
     8041 gacgacctta ccgttcaccg tgatggtgac atcggctgcc tgtatcgtcg cacttgcgag
     8101 cgccaatatt gccgccaata catacccacg tttgcaccat ttcatgagca tctccagtta
     8161 ctgatattca agagtgaagg tagccgtggc attgatatgc cctgcagtga caggcacctg
     8221 tgtcgccatt agccgggcgt aaaaattcag cgtatttggt ttacccggcg tcagggtcgt
     8281 ccacgaaagc gcggacgatg gagcattaag gggtatttga ttttgctgct cattcagaag
     8341 ctgtattccc agtcccgaag ccgctgacac cgtattttca agtgcaagca gattggcatt
     8401 gtggctatct gcaacgccgg taaacccaac ttttacggca gaaacggcat taccacaggg
     8461 tgacagcaaa atacgaaatg gaacaacagg agtcgtcgcg ccaatgttgt taaattgctt
     8521 cgccgcgttt tccatcagat caacagtaaa attggttgat tcagcggcca cactacagcc
     8581 gttatccctg acatagccgc ggatagtaat cgtgctatcc gcagccaaag cgtgactcac
     8641 cgccagccac aaaaaagcgc acagaagata aaaaggtttg tttctcatca cgccccctta
     8701 acgacattca gctgatagct gggttaataa ctgctgctga ctctctggtg gcagttgata
     8761 attggcgaca cagtgagcat tttcctcttc tccccatttc acctgaactt ttcccg
//
LOCUS       DAZGTR010000156         8764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGTR010000156 DAZGTR010000000
VERSION     DAZGTR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.158"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(339..602)
                     /gene="rpsT"
                     /locus_tag="ACHLCY_004295"
     CDS             complement(339..602)
                     /gene="rpsT"
                     /locus_tag="ACHLCY_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP0371415.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            705..924
                     /locus_tag="ACHLCY_004296"
                     /pseudo
     CDS             705..924
                     /locus_tag="ACHLCY_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
     gene            932..1873
                     /gene="ribF"
                     /locus_tag="ACHLCY_004297"
     CDS             932..1873
                     /gene="ribF"
                     /locus_tag="ACHLCY_004297"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP0371416.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1916..4732
                     /gene="ileS"
                     /locus_tag="ACHLCY_004298"
     CDS             1916..4732
                     /gene="ileS"
                     /locus_tag="ACHLCY_004298"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP0371417.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYMPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4732..5226
                     /gene="lspA"
                     /locus_tag="ACHLCY_004299"
     CDS             4732..5226
                     /gene="lspA"
                     /locus_tag="ACHLCY_004299"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP0371418.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5314..5763
                     /gene="fkpB"
                     /locus_tag="ACHLCY_004300"
     CDS             5314..5763
                     /gene="fkpB"
                     /locus_tag="ACHLCY_004300"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0371419.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5765..6715
                     /gene="ispH"
                     /locus_tag="ACHLCY_004301"
                     /gene_synonym="lytB"
     CDS             5765..6715
                     /gene="ispH"
                     /locus_tag="ACHLCY_004301"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP0371420.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6781..7695
                     /gene="rihC"
                     /locus_tag="ACHLCY_004302"
     CDS             6781..7695
                     /gene="rihC"
                     /locus_tag="ACHLCY_004302"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP0371421.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7862..8683
                     /gene="dapB"
                     /locus_tag="ACHLCY_004303"
     CDS             7862..8683
                     /gene="dapB"
                     /locus_tag="ACHLCY_004303"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP0371422.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         2004 a         2162 c         2485 g         2113 t
ORIGIN      
        1 taataaggag atatttatga atggttaaag tgtataggaa tttaattcaa aaatagagtg
       61 gtcttaagtt taatacctca atgtgtatca caatatccac attctttgta ggggagtctg
      121 gagattgagt agatattctt gttcagaatg tatcagccga tggttctacg attcttaagc
      181 cacgaagagt tcagaaagtg taacggcatg tctgcaaaag ccagcatctt ttgactatct
      241 ggcaaccggt agtgtgttct ctcacgcatc ataaaagcag caggcataaa aaaacccgct
      301 tgcgcgggct ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt
      361 gcagtcaggt tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc
      421 tgacggtcca cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca
      481 gcttcgatag ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg
      541 ttgtgcttac gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc
      601 aaggtccaac tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt
      661 ttagccttct tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt
      721 tacgtagtga tggcgcagga ttctaccagc ttgcagggtg tgaatacagc ttttccgcga
      781 taaaaattgc agcaggcggt cactttcttc ccgtgatttg cgccatggca atgaaaagcc
      841 acttctttct gatttcggta ctcaatcgcc ggttaacctt gaccgctgta ctaaggtata
      901 ctcggacgat tttcactgtt ttgagccaga catgaagctg atacgcggca tacataatct
      961 cagccaggcc ccgcaagaag ggtgtgtgct gactattggt aatttcgacg gcgtgcatcg
     1021 cggtcatcgc gcgctgttac agggcttgca ggaagaaggg cgcaagcgca acttaccggt
     1081 gatggtgatg ctttttgaac ctcaaccact ggaactgttt gctaccgata aagccccggc
     1141 acggctgacc cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg ttgattacgt
     1201 gctgtgcgtg cgtttcgaca ggcgtttcgc ggcgttaacc gcgcaaaatt tcatcagcga
     1261 tcttctggtg aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt tccgctttgg
     1321 cgctggtcgt gaaggcgatt tcttgttatt acagaaagct ggcatggaat acggcttcga
     1381 tatcaccagt acgcaaactt tttgcgaagg tggcgtgcgt atcagcagca ccgctgtgcg
     1441 tcaggcgctg gcggatgaca atctggctct ggcagaaagt ttactggggc acccgtttgc
     1501 catctccggg cgtgtagtcc acggtgatga attagggcgc actataggtt tcccgacggc
     1561 gaatgtaccg ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg tagaagtgct
     1621 gggcctcggt gaaaagccgt tacccggcgt ggcaaacatt ggaacacgcc caacggttgc
     1681 cggtattcgc cagcagctgg aagtgcattt gttagatgtt gcaatggacc tttacggtcg
     1741 ccatatacaa gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg cgtcgctgga
     1801 cgaactgaaa gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt ttgggctaac
     1861 aaaaccggct taagcctgtt atgtaatcaa accgaaacac ggaaccgaga atctgatgag
     1921 tgactataaa tcaaccctga atttgccgga aacagggttc ccgatgcgtg gcgatctcgc
     1981 caagcgcgaa ccgggaatgc tggcgcgttg gactgatgat gatctgtacg gcatcatccg
     2041 tgcggctaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt atgcgaatgg
     2101 cagcattcat attggtcact cggttaacaa gattctgaaa gacattatcg tgaagtccaa
     2161 agggctttcc ggttatgact cgccgtatgt gcctggctgg gactgccacg gtctgccgat
     2221 cgagctgaaa gtagagcaag aatacggtaa gccgggtgag aaattcaccg ccgccgagtt
     2281 ccgcgccaag tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca aagactttat
     2341 ccgtctgggc gtgctgggcg actggtcgca cccgtacctg accatggact tcaaaactga
     2401 agccaacatc atccgcgcgc tgggcaaaat catcggcaac ggtcacctgc acaaaggcgc
     2461 gaagccagtt cactggtgcg ttgactgccg ttctgcgctg gcggaagcgg aagttgagta
     2521 ttacgacaaa acttctccgt ccatcgacgt tgcttttcag gcggtcgatc aggatgcact
     2581 gaaagcaaaa tttgccgtaa gcaacgttaa cggcccaatc tcgctggtga tctggaccac
     2641 tacgccgtgg actctgcctg cgaaccgcgc aatctctatt gcacctgatt tcgactatgc
     2701 gctggtgcag atcgacggtc aggccgtgat tctggcgaaa gatctggttg aaagcgtaat
     2761 gcagcgtatc ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg cggagcttga
     2821 gttgctgcgc tttacccatc cgtttatggg cttcgacgtt ccggcaatcc tcggcgatca
     2881 cgttaccctg gatgccggta ccggtgccgt tcacaccgcg cctggccacg gcccggacga
     2941 ctatgtgatc ggtcagaaat acggcctgga aaccgctaac ccggttggcc cggacggcac
     3001 ttatctgccg ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag cgaacgacat
     3061 cgtcgttgcg ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa tgcagcacag
     3121 ctatccgtgc tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacgc cgcagtggtt
     3181 cgtcagcatg gatcagaaag gtctgcgtgc gcagtcactg aaagagatca aaggcgtgca
     3241 gtggatcccg gactggggcc aggcgcgtat cgagtcgatg gttgctaacc gtcctgactg
     3301 gtgtatctcc cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc acaaagacac
     3361 ggaagagctg catccgcgta ctctcgaact aatggaagaa gtggcaaaac gcgttgaagt
     3421 tgacggcatc caggcgtggt gggatcttga tgcgaaagag atcctcggcg atgaagctga
     3481 tcagtacgtg aaagtgccgg acacattgga tgtatggttt gactccggat ctacccactc
     3541 ttctgttgtt gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt atctggaagg
     3601 ttctgaccag caccgtggtt ggttcatgtc ttccctaatg atctccaccg cgatgaaagg
     3661 caaagcgccg tatcgtcagg tactgaccca cggctttacc gtggatggtc agggtcgcaa
     3721 gatgtctaaa tccatcggca ataccgtttc gccgcaggat gtgatgaaca aactgggcgc
     3781 ggatattctg cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg ccgtttctga
     3841 cgagatcctg aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg cgcgcttcct
     3901 gctggcaaac ctgaacggtt ttgatccagc aaaagatatg gtgaaaccgg aagagatggt
     3961 ggtactggat cgctgggccg taggttgtgc gaaagcggca caggaagaca tcctcaaggc
     4021 gtacgaagca tacgatttcc acgaagtggt acagcgtctg atgcgcttct gctccgttga
     4081 gatgggttcc ttctacctcg acatcatcaa agaccgtcag tacaccgcca aagcggacag
     4141 cgtggctcgt cgtagctgcc agactgcgct gtatcacatc gctgaagcgc tggtgcgctg
     4201 gatggcacca atcctctcct tcaccgctga tgaagtgtgg ggctatatgc cgggcgaacg
     4261 tgaaaaatac gtctttaccg gtgagtggta cgaaggcctg tttggtctgg cagacagtga
     4321 agcgatgaac gatgcgttct gggacgagct gttgaaagtg cgtggcgaag tgaacaaagt
     4381 cattgagcaa gcgcgtgccg ataagaaagt gggtggctcg ctggaagcgg cagtaacctt
     4441 gtatgcagaa ccggaactgg cggcgaaact gaccgcgctg ggcgatgaat tacgatttgt
     4501 cctgttgacc tccggcgcta ccgttgcaga ctataatgac gcacctgctg atgctcagca
     4561 gagcgaagta ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg agaagtgccc
     4621 acgctgctgg cactacaccc aggatgtcgg caaggtggcg gaacacgcag aaatctgcgg
     4681 ccgctgtgtc agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct gatgagtcaa
     4741 tcgatctgtt caacagggct acgctggctg tggctggtgg tagtcgtgct gattatcgat
     4801 ctgggcagca aatacctgat cctccagaac tttgctctgg gggatacggt cccgctgttc
     4861 ccgtcgctta atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt ccttgccgat
     4921 agcggcggct ggcagcgttg gttctttgcc ggtattgcga ttggtattag cgtgatcctg
     4981 gcagtgatga tgtatcgctc gaaggccacg cagaagctaa acaatatcgc ttatgcgctg
     5041 attattggcg gcgcgctggg caacctgttc gaccgcctgt ggcacggctt cgttgtcgat
     5101 atgatcgact tctacgtcgg cgactggcac ttcgccacct tcaaccttgc cgatactgcc
     5161 atttgtgtcg gtgcggcact gattgtgctg gaaggttttt tgccttctaa agcgaaaaaa
     5221 caataataaa ccctgccgga taaggcctac agattgctgc gaaatcgtag gccgcatccg
     5281 gcaaaaatcc tcaaaatata agagcaaacc tgcatgtctg aatctgtaca gagcaatagc
     5341 gccgtcctgg tgcacttcac gctgaaactc gatgatggca ccaccgctga gtctacccgc
     5401 aacaacggta aaccggcgct gttccgcctg ggcgatgctt ctctttctga agggctggag
     5461 caacacctgt tggggctgaa agtgggcgat aaaaccacct tctcgctgga gcccgatgcg
     5521 gcgtttggcg tgccgtcacc ggacctgatt cagtacttct cccgccgtga atttatggat
     5581 gcaggcgagc cagaaattgg cgcaattatg ctttttaccg caatggatgg cagtgagatg
     5641 cctggcgtga tccgcgaaat taacggcgac tccattaccg ttgatttcaa ccatccgctg
     5701 gccgggcaga ccgttcattt tgatattgaa gtgctggaaa tcgatccggc actggaggcg
     5761 taacatgcag atcctgttgg ccaacccgcg tggtttttgt gccggggtag accgcgctat
     5821 cagcattgtt gaaaacgcgc tggccattta cggcgcaccg atatatgtcc gtcacgaagt
     5881 ggtacataac cgctatgtgg tcgatagctt gcgtgagcgt ggggctatct ttattgagca
     5941 gattagcgaa gtaccggacg gcgcgatcct gattttctcc gcacacggtg tttctcaggc
     6001 ggtacgtaac gaagcaaaaa gtcgcgattt gacggtattt gacgcgacct gtccgctggt
     6061 gaccaaagtg catatggaag tcgcccgcgc cagtcgccgt ggcgaagaat ctattctcat
     6121 cggtcacgcc gggcacccgg aagtggaagg gacaatgggc cagtacagta acccggaagg
     6181 gggaatgtat ctggtcgaat cgccggacga tgtgtggaaa ctgacggtca aaaacgaaga
     6241 gaagctctcc tttatgaccc agaccacgct gtcggtggat gacacgtctg atgtgatcga
     6301 cgcgctgcgt aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca tctgctacgc
     6361 cacgactaac cgtcaggaag cggtacgcgc cctggcagaa caggcggaag ttgtgttggt
     6421 ggtcggttcg aaaaactcct ccaactccaa ccgtctggcg gagctggccc agcgtatggg
     6481 caaacgcgcg tttttgattg acgatgcgaa agatatccag gaagagtggg tgaaagaggt
     6541 taaatgcgtc ggcgtgactg cgggcgcatc ggctccggat attctggtgc agaatgtggt
     6601 ggcacgtttg cagcagctgg gcggtggtga agccattccg ctggaaggcc gtgaagaaaa
     6661 cattgttttc gaagtgccga aagagctgcg tgtcgatatt cgtgaagtcg attaagtcat
     6721 tagcagccta agttatgcga aaatgccggt cttgttaccg gcatttttta tggagaaaac
     6781 atgcgtttac ctatcttcct cgatactgac cccggcattg acgatgccgt cgccattgcc
     6841 gccgcgattt ttgcacccga actcgacctg caactgatga ccaccgtcgc gggtaatgtc
     6901 tcggttgaga aaactacccg caatgccctg caactgctgc atttctggaa tgcggagatt
     6961 ccgctcgccc aaggggccgc tgtgccgctg gtacgcgcac cgcgtgatgc ggcatctgtg
     7021 cacggcgaat cgggaatggc tggctacgac tttgttgagc acaaccgaaa gccgctcggg
     7081 ataccggcgt ttctggcgat tcgggatgcc ctgatgcgtg caccagagcc tgttaccctg
     7141 gtggccatcg gcccgttaac caatattgcg ctgttacttt cacaatgccc ggaatgcaag
     7201 ccgtatattc gccgtctggt gatcatgggt ggttctgccg gacgcggcaa ctgtacgcca
     7261 aacgccgagt ttaatattgc tgccgatcca gaagctgctg cctgtgtctt ccgcagtggt
     7321 attgaaatcg tcatgtgcgg tttggatgtc accaatcagg caatattaac tcctgactat
     7381 ctctctacac tgccgcagtt aaaccgtacc gggaaaatgc ttcacgccct gtttagccac
     7441 taccgtagcg gcagtatgca aagcggcttg cgaatgcacg atctctgcgc catcgcctgg
     7501 ctggtgcgcc cggacctgtt cactctcaaa ccctgttttg tggcagtgga aactcagggc
     7561 gaatttacct caggcacgac ggtggttgat atcgacggtt gcctgggcaa gccagccaat
     7621 gtacaggtgg cattggatct ggatgtgaaa ggcttccagc agtgggtggc tgaggtgctg
     7681 gctctggcgt cgtaacctgt cacatgttat tggcatgcag tcattcatcg actcatgcct
     7741 ttcactgata tccctccctg tttatcatta atttctaatt atcagcgttt ttggctggcg
     7801 gcgtagcgat gcgctggtta ctctgaaaac ggtctatgca aattaacaaa agagaatagc
     7861 tatgcatgat gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta tgggccgcca
     7921 gttgattcag gcggcgctgg cattagaagg cgtgcagctg ggcgcggcgc tggagcgcga
     7981 aggatcttct ttactgggca gcgacgccgg tgagctggct ggtgcaggga aaacaggcgt
     8041 taccgtgcaa agcagcctcg atgcggtaaa agatgatttt gatgtgttta tcgattttac
     8101 ccgtccggaa ggtacgctga accatctcgc tttttgtcgc cagcatggca aagggatggt
     8161 gatcggcact acggggtttg acgaagccgg taaacaggcg attcgtgacg ccgccgccga
     8221 tattgcgatt gtctttgccg ccaattttag cgttggcgtt aacgtcatgc ttaagctgct
     8281 ggagaaagca gccaaagtga tgggtgacta caccgatatc gaaattattg aagcacatca
     8341 tagacataaa gttgatgcgc cgtcaggcac cgcactggca atgggagagg cgatcgccca
     8401 cgcccttgat aaagatctga aagattgcgc ggtctacagt cgtgaaggcc acaccggtga
     8461 acgtgtgcct ggcaccattg gttttgccac cgtgcgtgca ggtgacatcg ttggcgaaca
     8521 taccgcgatg tttgccgata ttggcgagcg tctggagatc acccataagg cgtccagccg
     8581 catgacattt gctaacggcg cggtaagatc ggctttgtgg ttgagtggta aggaaagtgg
     8641 tctttttgat atgcgagatg tgcttgatct caatagtttg taaccacaaa atatttgtta
     8701 tggtgcaaaa ataacacatt taatttattg attgtaaagg gctttaattt ttggcccttt
     8761 tatt
//
LOCUS       DAZGTR010000157         8689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGTR010000157 DAZGTR010000000
VERSION     DAZGTR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.159"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            444..1514
                     /locus_tag="ACHLCY_004304"
     CDS             444..1514
                     /locus_tag="ACHLCY_004304"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP0371423.1"
                     /translation="MLQYTSVEICAGAGGQALGLHNAGFTHRALVEIDPAACETLRLN
                     NELHSLGWENIIEGCVKHFAEHTAYNFSDIDLVAGGVPCPPFSKAGKQLGKDDERDLF
                     PTALKIVNIIKPKAVMIENVSGLLDPKFKQYRQELDHAFSAMGYKTFWKLHNASDYGV
                     PQLRPRVLLVALRGKYSAHFRWPSETLIPPTVGDALFDLMSQGGWEGAEAWRKAANNI
                     APTLVGGSHKHGGPDLGPTRAKKAWQALGVNGHSIADSSPEKGFTGYIGRDGNIRAGL
                     ENMPRLTVRMAARIQGFPDWWEFSGKKTAAYRQVGNAFPPPVAEATGRQIRKALEAVD
                     LMKKKQLASPLESNDVQSLRIA"
     gene            complement(1493..2152)
                     /locus_tag="ACHLCY_004305"
     CDS             complement(1493..2152)
                     /locus_tag="ACHLCY_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0371424.1"
                     /translation="MELFENYLSALPKKKRLSKVKLIILKRLWVSDDFDFPKPWVPST
                     ELLELTGQKYFDRRTRELRDELGCDLESSYIPEFSGHAWRLKSSMIAPPMEREYLTES
                     QRSSLFSNCSNTCATCGKTVAAGVRGLQADHKIPLSRGGGNELTNWQALCHNCNVGKR
                     RACEGCTEDCYTCSWAFPEKFGITTMLHLPEASLRRAQQLADAKGFSLDEIVDYAIRK
                     L"
     gene            2699..3058
                     /locus_tag="ACHLCY_004306"
     CDS             2699..3058
                     /locus_tag="ACHLCY_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371425.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGDTNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            complement(3051..3593)
                     /locus_tag="ACHLCY_004307"
     CDS             complement(3051..3593)
                     /locus_tag="ACHLCY_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HFP0371426.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSKLHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNEGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPAENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSRINDRLQAIN"
     gene            complement(3714..3899)
                     /locus_tag="ACHLCY_004308"
     CDS             complement(3714..3899)
                     /locus_tag="ACHLCY_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371427.1"
                     /translation="MTPREIALLTIAKLEHGGHQLTQADQREIERSVNADIARRDRFR
                     EMMRAPAYQWKKPAPRR"
     gene            complement(4195..4476)
                     /locus_tag="ACHLCY_004309"
     CDS             complement(4195..4476)
                     /locus_tag="ACHLCY_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0371428.1"
                     /translation="MIRDRKAEELESKGLYRRAAARWMEVMLLCTEDDDREWIKRRRE
                     TCLENVKRPPVKVEEFGDLHKAVTETQHRMGIAQPNGNAFRLNGGKRQR"
     gene            complement(4708..4899)
                     /locus_tag="ACHLCY_004310"
     CDS             complement(4708..4899)
                     /locus_tag="ACHLCY_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371429.1"
                     /translation="MTPREIELLTIAKLEHGGHQLSPAELRELRRQLAEGPVIARRYR
                     EMMTSPAYRWSKPAPLRAR"
     gene            4913..5224
                     /locus_tag="ACHLCY_004311"
     CDS             4913..5224
                     /locus_tag="ACHLCY_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371430.1"
                     /translation="MVIGIILLARVILHWPEPLCVKSMRELHMLCVKLQLKMSCSGGN
                     FLVNSVLVDTNMDTSLLDTTQSQIVTMLNLKQRSLYAPYQIKRSHAQNGLQENGNLLM
                     G"
     gene            5178..5336
                     /locus_tag="ACHLCY_004312"
     CDS             5178..5336
                     /locus_tag="ACHLCY_004312"
                     /inference="COORDINATES: protein motif:HMM:NF017717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0371431.1"
                     /translation="MLRTGFKKTAIYSWVKTGTFPQPVKIGRSARWSLEEVEAWIQNK
                     LDSRAGNQ"
     gene            6331..6720
                     /locus_tag="ACHLCY_004313"
     CDS             6331..6720
                     /locus_tag="ACHLCY_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371432.1"
                     /translation="MIAILNKAKGRVGVNAQAVIMVFLGLIVAKLYPDWWKGALATVA
                     IYLLLSVVKRSFVKTVNQAKRFSFKNLINYITGLIFCLSILIASAFLPVSIANFFGAP
                     YEVGYLFSVFCLVLSLLYLWMRAKNKN"
     gene            6791..7018
                     /gene="ykgL"
                     /locus_tag="ACHLCY_004314"
     CDS             6791..7018
                     /gene="ykgL"
                     /locus_tag="ACHLCY_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFP0371433.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(7053..7193)
                     /gene="ykgO"
                     /locus_tag="ACHLCY_004315"
     CDS             complement(7053..7193)
                     /gene="ykgO"
                     /locus_tag="ACHLCY_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP0371434.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(7193..7456)
                     /gene="ykgM"
                     /locus_tag="ACHLCY_004316"
     CDS             complement(7193..7456)
                     /gene="ykgM"
                     /locus_tag="ACHLCY_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0371435.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(7820..7921)
                     /gene="ykgR"
                     /locus_tag="ACHLCY_004317"
     CDS             complement(7820..7921)
                     /gene="ykgR"
                     /locus_tag="ACHLCY_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP0371436.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(8395..8484)
                     /locus_tag="ACHLCY_004318"
                     /pseudo
     CDS             complement(8395..8484)
                     /locus_tag="ACHLCY_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2328 a         1961 c         1942 g         2458 t
ORIGIN      
        1 cgtagatatc ttacgagcac gacaaacaac cgacatgttg cgacgaagcc aaagctgaat
       61 tccacaagca gcgtgccaat ctttcttatt catttgttct ctgcagaatg cgtctgctat
      121 ttacagcgga cgcaatgcat cttacggaca aaaaatcatg taaacagata gtagttcttt
      181 tcgtactcag ctaaggcatt ttattcagtc cacatgagat tctgcctgtc tgatcaagag
      241 aatgccaata gtactgtcga atgttagtta tttgataaat ataggattcc aaaacaaagt
      301 tgtagattgc cttgtcattc agcaatcaag taactaagct gaccggttca atagcgacgg
      361 tactacttgt tttgtacaat ttgttgcgta atggtctctg atcagataaa catacaactg
      421 tttttatata caggtttaat gacatgttac aatacacatc tgtagagatt tgtgcagggg
      481 caggaggtca agctctaggg ttacataacg ctggcttcac tcaccgcgcg ctcgtcgaga
      541 ttgatcctgc tgcctgcgaa actcttcgcc tcaacaatga gctccattct ttaggttggg
      601 aaaatatcat agagggttgc gttaagcatt ttgctgaaca tactgcatat aatttttctg
      661 atattgactt ggtcgcaggg ggagtgccct gcccgccatt ctcaaaagca gggaaacaac
      721 taggcaaaga tgatgaacgt gatttgtttc caacagcgct caaaattgtg aacatcatta
      781 agccaaaagc agtaatgatt gaaaatgttt caggtttatt agacccaaag tttaaacaat
      841 accgacaaga actagatcat gctttctctg caatgggtta taaaaccttt tggaaacttc
      901 ataacgcttc tgattacgga gttcctcagc ttcgtccccg agttctttta gttgcattgc
      961 gtggtaagta ttccgctcat tttcgttggc cgagtgaaac gttgatacca ccgactgtgg
     1021 gtgatgctct ctttgactta atgtcacagg gtggctggga aggagccgag gcatggagaa
     1081 aggccgctaa caatatagcc cctacacttg ttggcggttc ccataagcat ggaggtccag
     1141 accttggacc gacgagagcc aaaaaagctt ggcaagcgct tggagtcaac gggcattcca
     1201 tagctgatag tagccctgaa aaggggttta ctgggtatat tggaagagac ggaaatattc
     1261 gagcaggtct tgaaaatatg cctcgtttga cggtgagaat ggctgcacga atccaaggtt
     1321 tcccggactg gtgggaattc tctgggaaaa aaacagctgc ttatcgtcaa gtaggtaacg
     1381 cttttcctcc tcctgttgcc gaagcaacag gaaggcaaat tcgtaaagca ctcgaagcgg
     1441 ttgatctaat gaaaaaaaaa cagttggcat caccactgga atcgaatgat gttcaaagct
     1501 tgcgtattgc gtagtcaacg atttcgtcta aagaaaaacc ctttgcatca gctagttgtt
     1561 gggctcttct taaactagct tctggcagat ggagcattgt tgttatccca aatttttcag
     1621 gaaatgccca tgagcatgtg taacaatctt cagtacatcc ctcacatgct cttctcttac
     1681 ctacgttgca attgtggcaa agagcttgcc aattggttaa ctcatttcca ccaccgcgtg
     1741 acaaaggtat tttgtgatcc gcttgtagcc ccctgacccc agcggcaaca gttttaccac
     1801 atgttgcaca tgtattgctg cagttagaaa ataaactgga tcgttgtgat tctgtcagat
     1861 attccctttc cattggcggg gcaatcatgc ttgatttcag ccgccaagca tgtcccgaga
     1921 attccggaat gtacgagctt tcgagatcac agcctagttc atcacgcaac tctcttgttc
     1981 gtctatcgaa gtacttttgc cctgtcaatt ccagtaattc tgtcgatggg acccaaggtt
     2041 taggaaaatc aaaatcatct gaaacccata atctctttag gataatcaac ttgactttac
     2101 tcaatcgctt ttttttaggt aacgctgaca aataattttc gaataattcc atactatctc
     2161 tcgaaagaaa ccttaatctc gcaagggatt acaccataga tggaagtaga caggaaaggg
     2221 ctatctaaaa gggcaggcat atagagcaat aatcaatgaa acaacctgta caggcctgct
     2281 aagctgcccc cccaaatgtc caacatcaat atcagaaata ttgtttcatc aagatgaaaa
     2341 aatcaacaag actgagccaa tctgagatac ggatgctcta ttgttgattt atgttgacga
     2401 aaattactta cacatggagt tacacggaaa aaatcatgat gcagacaaag agaggattta
     2461 atcgtaattg cttgatataa atggtgcgat aataggaaca atacacatag gcgataagct
     2521 ttgtttttgt tgcctttttt attgttcatt ttttatagat acccccaaaa gtacccccat
     2581 atctttttcc gcacctgaaa tttggttgtt attcatacag catatcaggc ggtaacgctc
     2641 tccctttaac ccacttattg tatgatagtg ctatcccaca ataaggaatc agccagccat
     2701 gagaatgacc agtcgcaaaa aagagatact tagctacttt gaaccggata acctcgaatg
     2761 ggtgactggt gagattggtg cgccgccgtt cgatgtgtcg ggtgtggcct acctgcttca
     2821 tggcatggtg tcgttcgata agcgtcacca gctcgaatca acccggcgca cactggaaag
     2881 catggttgct ggtggactgc ttgagcgggt gacagtgtat gaatcgcgcc agatacggcg
     2941 cggcggtgat actaacgcaa cggtcgtgcg ctacggttta cccggtcagt gcgctgttat
     3001 gcgcgatact ggcggcgctg acaacgcaat atcaggtgaa tacatgcggg tcagttaata
     3061 gcctgcaatc tgtcgttgat gcggctcagg tcgtaaaccc ggcgttcaat atccagcatc
     3121 aggaaattgt ggatctcggc ctgctcagga ttatcagact ctctggcctg taatagcaat
     3181 tcattgtaac catgcaagcc gcctgagttt tccgccgggg tggtgccttt cccacgcagg
     3241 cagctaatgg gctgattact cgtatcgtgt gtttgcttct ccagcacaat gacgcaatcc
     3301 caccagtcgc caaagtcata cgtgtaaacc agcttgtcac cctcattctg gagcgccgcc
     3361 catagtggcg tgattgccgc gttacccatg ctttcatcct caccccatac gcgatactcg
     3421 ctacctggat cacctttacg ccctttctcg aacatataca ggtgttcgtc attccagcca
     3481 aaagctaact ggatcacggc atgaagttta ctaaagggga tttgctctgg aaccagcaca
     3541 cgccgccata taggtggttt gctgtctcgg atggttatct ttagctggta catgcaaatc
     3601 ctgaataaat gcgaatcgga aggataacga tactgacgta ctcacaattc tgtaaaggtg
     3661 aataaaggaa atttgcttaa tacgcataag ttacatgtgg aataatccca tatctacctg
     3721 cgcggcgctg gcttcttcca ctggtaggca ggtgctcgca tcatttcgcg gaacctgtcg
     3781 cgccgggcta tatcggcatt aactgatcgt tctatctccc gttgatctgc ctgtgtaagc
     3841 tggtggcctc cgtgctcaag tttggcgatg gtcaataagg ctatttcgcg tggtgtcata
     3901 cgtcataccc tctggaagga tgaaacacgc ccggattaaa tttcattttt cgcgggtata
     3961 aaaaaacgat ggggcgggca gtcccggata gtcagggctg tagagatttg actaccccct
     4021 ccccactgga ttcaggttac tgctattccg ttaagcgctg cttacgggga tagctgcgtt
     4081 gtgattttga ccattcaggt cgccgctatt tcctcaccga ttgggtgcta cgcgcgcgta
     4141 cttgtcccga aataccagaa accgccagcc agaggatgat ttccctctgg tggtctacct
     4201 ttgcctcttg ccgccattta accggaaggc gttaccgttc ggttgcgcta tccccatgcg
     4261 gtgttgcgtt tcggtaacag ctttatgcag gtcgccaaat tcctcaacct tcacgggcgg
     4321 gcgcttcacg ttctccagac acgtttcacg gcggcgcttt atccattccc gatcatcgtc
     4381 ctcggtgcat aacagcatga cttccatcca tcgtgcggca gctctccggt atagcccttt
     4441 tgactccagc tcttcagctt tcctgtccct tatcatttct gcctctcaga ttatatagat
     4501 aagcaaaaag gtgttggttc agttggttca gttggttcaa tttgtaaaga gtattgtttt
     4561 taaaagaatt attcgcaaaa agtgaaccaa cacaccccat ttttgaacca acaaaaagag
     4621 gccgtgaacc aacacgccgt ttaaaaaccg ctaattatga gggacatcca ccagcacagg
     4681 gcttgtctag tgctggttag gtgctgctca ccgcgcacgg agcggcgctg gtttgctcca
     4741 gcgatatgcg gggctggtca tcatttcgcg gtaccgcctg gctattacag ggccctctgc
     4801 cagctggcgc cttaattccc gcagttctgc cgggctgagc tggtggccgc catgctccag
     4861 cttggcaatg gtcagcagct cgatttctcg tggtgtcatg gattcccctc tgatggtgat
     4921 cggcattata ttgctagcca gggtgattct tcactggcca gagccgttgt gcgtgaagtc
     4981 tatgcgtgaa ctccatatgc tatgtgttaa gttacagctc aaaatgtcct gttccggtgg
     5041 taactttttg gtaaacagcg ttttggtgga taccaacatg gatacctcgc ttttggatac
     5101 cacccaaagc cagattgtca ctatgcttaa tctaaagcag aggagcctct atgcgcctta
     5161 tcaaattaag cgaagtcatg ctcagaacgg gcttcaagaa aacggcaatc tactcatggg
     5221 ttaagacggg cacgtttcca cagccagtta aaattggtag atcagctaga tggtcgcttg
     5281 aggaggttga agcttggatc cagaacaaac ttgattcgcg ggctggcaac caatgattag
     5341 ctttaatatc gagtggtgga tagtccatgg acaagctgat gggcgcactc gacgaaaacg
     5401 atgcgccagg cggactgtgt cgctcataaa ccgggagttg gccagaggac ttagttgtcc
     5461 tttgcaattc tgcaggttcg cacgtgaaag tagccaaaac cctgcgaacc tgagtgcaaa
     5521 cctgcaactg cgaaccactg aggtggcctc tcatagctgg tggtcatact ggatattaat
     5581 caggcctgcc acaaccataa caatccataa cagacgcatc gcaccaacaa ttactaatag
     5641 gtccgctcgc aacactaatt ttgactaagc ctgaacgcaa tgctcagaaa gaacgaagaa
     5701 tggaatgaat cgtaccatca tgttgctcca tgatttttac gttcaagaca gttgcttaat
     5761 ctcgcctcgc aattcagccc ccttgccgtt atcgagaagt ctttctcctt acggttaatg
     5821 ctatcggtct tcatattacc agcagcatac gccatccgat tatttcgatg ctacgcgcgc
     5881 gtacttgtcg caaaatacca ggcaaaggcc agattcagaa cgtcgtgata accaccagta
     5941 caacatgaga ggtcatgtaa cgcgatttat cacaccagat acatgtgatt ggtgtacccg
     6001 cataaaacat cgtcatagcg cgtcctgtgc gcatatacgc cgataatcaa cacatcacca
     6061 ccactctgtc tcatgtagag aacagtttta tggtatcccc ccgccatcgt gtgcatactg
     6121 gtcggacgta gttgcaaaat tttttgcaac tgatacccct cgcgcgcgta cttgtctcga
     6181 aattggtgtt ttgctgtggc agtgatatca gaaaaatctt cctgtttcac ataaagcgga
     6241 tatgcgcgta ctgtaacggc cccatcaacg gggatcggat ttgaaatccc gacccaagct
     6301 atgtaccaaa tgccatgtct tggatatatg atgattgcca ttctaaacaa agctaaaggg
     6361 agggttggtg tgaatgctca ggctgtcatc atggttttcc ttggtctgat tgtcgcaaaa
     6421 ctatatccag actggtggaa aggggcatta gcaactgtcg ccatctattt gcttttatcc
     6481 gtcgttaagc gatcgttcgt taagaccgta aatcaagcaa aacgtttctc atttaagaat
     6541 ttaataaact atattactgg gcttattttt tgcctttcaa ttttgatcgc ctcagctttt
     6601 ttgcccgtat caatagctaa tttttttggc gcaccatatg aggtaggtta tctattctct
     6661 gttttttgtc ttgttctttc ccttctttac ctatggatga gagcaaagaa caaaaattag
     6721 ccatccatga cgtttttatg ttagtaccaa cagatttacc acatttatca tcttaacagt
     6781 ttgagtgaaa atgcaggctg cgccgcaaag ggaggagact gagtggcgtg ttcagtctaa
     6841 aagagggttg atgccagcat acaggggcga agctggtcaa caagtcaaca ttaagataat
     6901 ggaatattcg gagagaaacg tcaggcagtt ggcctcaaat gaacaggaag aatatatccc
     6961 caggaaaatt aatgttggtg ttataaacac accaacatta atccgatctg attattaatc
     7021 agacaaatat cgttaattcg tcgaattttg aatcaacgtt ttttcttacg accctgaacg
     7081 gccttaaaac gtggattaga tttacaaatc acatacaacc gtccttttcg cttcacaatc
     7141 tgacagtctg gatggcgttc ttttgcggta cgcagagagt taagaacttt catcacgccc
     7201 cctttttcgt gctaacaaaa cgaccaaaac gttgggtgaa tcgtgcaaca tttccttctg
     7261 atgccactgt tctcagcttc cctgtataga acgggtgcga tttagaagag acatcaattg
     7321 tcacgtacgg atacgttacg ccatccagct caatctcacg gtctgttttg atagtcgagc
     7381 cgattttaaa gtactcatca acactggtgt cgtggaacac cacagtacga tactcaggat
     7441 ggatattggg cttcatcatt tttacctgtt atgttataac ataaccgtaa agatacatgc
     7501 tcatccggat catttcaaca cccaatccct gtgcccattt gtggtatgtg gaatttcccc
     7561 aacggggaag tctggcattg ttagcagccg ttggcgacgc tcccataaac gcctggatga
     7621 gtgattgatg atggttttgc tgagagtatg gatttttcga gaatggaggt tatctccact
     7681 ttactggccg atgatttatc gcagtatgat tgattacctg acagcgtgta ttcctctccc
     7741 acggatataa taattactta caaaaaggga gaggatgcat attttaaata tcactgaagt
     7801 gaacagttta ttgccgttat taatagaaat ggagaaataa ataggcgtat tctacaattg
     7861 cgacaaaaac aacgatatta atcagtttat gactgatttg ttgtacttta ttctctttca
     7921 ttggtacttc ctcgctttaa aaaagagtgc acttcgtaag tgcccttata taaataacga
     7981 gtttggtcaa ccaatttttt gacatgtatc acaaatttga atagatgtat tacatcagct
     8041 atcttttatt gcaccaacgt cattgatata tgtcgactga agtcagttcc gggaatgagt
     8101 ctgatctcaa gactggccca gtccgggcgt tgattggcgc tgaggagcat acctcatttc
     8161 atcataatgt cgtatctcct ggggtgttat acaagatatc gttgttggta acctgggaaa
     8221 ggaattgagt tctattaaac cgtcaactat gccggataca tactggatta cactgcaggc
     8281 acgccttatg agagaacgtg ccgcagtgac gggttaatta tctgaaagaa tttgtgaggc
     8341 tgtatcggtt actcattgat ttgatagttt tactctcggg agaataatag atatttaatc
     8401 cattaacgga aaccagccag ttcctttcga tgcctgaatt tgatcccata gtttatccgg
     8461 aatggttaat tcatgaacaa gttgtgttat cgttcatgag aagcataacg taaagggaaa
     8521 agctcgatta gacggcagaa tttgtcaggg gttatgaacg aaattcataa atctgtttga
     8581 gtgttgcgat gggtagtgca agttcgatat ctccgcaatt tacagtccga tgaaggaaaa
     8641 tgaatatcca taaaaaatat attggtttat cctggcatat atacctatt
//
LOCUS       DAZGTR010000158         8624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGTR010000158 DAZGTR010000000
VERSION     DAZGTR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.160"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..195
                     /locus_tag="ACHLCY_004319"
                     /pseudo
     CDS             1..195
                     /locus_tag="ACHLCY_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            718..1233
                     /gene="ehxC"
                     /locus_tag="ACHLCY_004320"
                     /gene_synonym="hlyC"
     CDS             718..1233
                     /gene="ehxC"
                     /locus_tag="ACHLCY_004320"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP0371437.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            1235..4231
                     /gene="ehxA"
                     /locus_tag="ACHLCY_004321"
     CDS             1235..4231
                     /gene="ehxA"
                     /locus_tag="ACHLCY_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP0371438.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTFLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKDNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFVFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKSMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            4281..6401
                     /gene="ehxB"
                     /locus_tag="ACHLCY_004322"
                     /gene_synonym="hlyB"
     CDS             4281..6401
                     /gene="ehxB"
                     /locus_tag="ACHLCY_004322"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP0371439.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            6405..7844
                     /gene="ehxD"
                     /locus_tag="ACHLCY_004323"
     CDS             6405..7844
                     /gene="ehxD"
                     /locus_tag="ACHLCY_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP0371440.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVIIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
     gene            complement(7971..>8624)
                     /locus_tag="ACHLCY_004324"
     CDS             complement(7971..>8624)
                     /locus_tag="ACHLCY_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc48 family transposase"
                     /protein_id="HFP0371441.1"
                     /translation="LRRQSRNEGLPFVNAKRVYRIMSENNLLLLHDKPSRQQREHKGR
                     ISVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCAREAIDWAASTGGYDKATVQDVML
                     GAIEKRFGDKVPEQSIQWLTDNGSAYRAHETRQFARELNLKPCTTAISSPQSNGMAER
                     FVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPREYRRQRVMLT"
BASE COUNT         2676 a         1422 c         1928 g         2598 t
ORIGIN      
        1 cagcgcgatg actggctgaa gagagagata cagcgcgtat acgatgagaa tcatcaggta
       61 tacggtgtgc gtaaagtctg gtgccagttg ttacgcgaag gtatcagggt ggccagatgt
      121 acagtggcgc gcctcatggc ggttatggga cttgccggtg ttctccgggg taaaaaggtc
      181 cgtactaccg tcagccgatt tacgggccgt aagtaacgaa gttggatgca aatgtcagat
      241 cgtgtgcgcc tgttatggct cggctggtaa gagagcctta taggcgcatt tgaaaaaact
      301 ccagctatac cggaagatat ttattattaa caggagaggt ttgcttttct gtgtaggttt
      361 agattttgtg tttgcagggt aaacaaaact gattaactcg tcatttcggt aactgaaata
      421 gtttgctgta ttctgtttac aatctcttta ataaggcatt tgtcacgtgg ctattcatat
      481 gaaaatcata cgttatctta caaatcaatc atctgagtgt tataatataa cttagctgtg
      541 atatgtgtaa gaatgtttag gcaatatatt attatcgcta atgttgtttt ggttgggaga
      601 gattctcctt gcggtagctt tttgttttta gatgcttctt gcttaaaaag atataatttc
      661 tgttctttta tataggattc tttacattta ctgagttttc ttatttattt aaacattatg
      721 aaatctaatg cttttgacgt catggggaag gttgcatggc tatgggcctg ttctcctctg
      781 cataaaaaat ggccattatc tgtttttgct attaatgtca tacctgctat tcagacaaat
      841 caatttgctc tcttaataaa ggatgagctt cctgtagctt tttgtagctg ggccagttta
      901 gatctggaat gtgaggtaaa atatataaat gatgtaacat ctttgtatgc aaaagactgg
      961 atgtcgggag aaagaaagtg gtttattgac tggatagctc catttggaca caacatggag
     1021 ttatataaat acatgaggaa aaagtatcct tatgagttat tcagagcaat aaggttagat
     1081 gagagcagta aaacaggaaa aatagctgaa tttcatggag gggggataga taaaaaactg
     1141 gcatcaaaaa tattcaggca ataccatcat gaacttatgt ctgaagtaaa aaacagacaa
     1201 gattttaatt ttaatattga gaaagaaaac taatatgaca gtaaataaaa taaagaacat
     1261 tttcaataat gcgacattga ctacaaaatc agcatttaat acagcatcat caagcgtacg
     1321 ttccgctggc aaaaaactca tattattaat acctgataat tatgaagctc agggcgtggg
     1381 tattaatgag ttggtcaaag ctgctgatga gcttggaata gaaatacacc gtactgaacg
     1441 agatgataca gcgattgcaa accagttttt tggtgcagca gaaaaagttg taggattaac
     1501 tgaacgtggt gttgcaatat tcgcaccaca acttgacaaa cttctgcaga agtatcagaa
     1561 agttgggagt aaaataggaa gaaccgctga aaatgtaggt aataatctgg gaaaagccgg
     1621 aacagttctc tcagcactac agaattttac ggggattgct ttatcaggca tggctcttga
     1681 tgaattgctg agaaaacaac gggaaggaga ggatataagt cagaatgata ttgccaaaag
     1741 tagtattgaa cttattaatc agcttgtaga tacagtatca agtataaaca gtaccgttga
     1801 ttcattttct gagcagctta accagcttgg ctcattttta tccagtaaac ctcgcttaag
     1861 ttctgttggt gggaaattac aaaatttacc agacctgggc tccctggggg atgggctgga
     1921 tgttgtctcc ggaattcttt ctgctgtatc agcaagcttt attctgggaa acagtgacgc
     1981 acatacagga acaaaagctg cagcgggtat cgaactgaca actcaggttc ttggaaatgt
     2041 tggtaaagct gtttcgcaat atattctggc tcagagaatg gcacaggggt tatcgacaac
     2101 agctgcaagt gcgggtctga tcacatcggc tgttatgctg gctatcagtc ctctttcttt
     2161 cctggctgct gcagataaat ttgagcgagc taagcagctt gaatcatatt ctgaacgatt
     2221 taaaaaattg aattatgaag gggatgcttt actcgcagcc tttcataaag aaaccggagc
     2281 tatagatgca gccctgacaa caataaatac tgtcctgagt tctgtatctg cgggagttag
     2341 tgcagcctcc agtgcatccc tcataggggc cccgataagc atgctggtga gtgcattaac
     2401 cggtacgata tctggcattc tggaagcatc aaaacaggct atgtttgagc acgttgcaga
     2461 gaaattcgct gctcggatca atgaatggga aaaggagcat ggcaaaaatt attttgagaa
     2521 tggctatgac gcaagacatg ctgcgttttt agaagactct ctgtctttgc ttgctgattt
     2581 ttctcgtcag catgcagtag aaagagcagt cgcaataacc cagcaacatt gggatgagaa
     2641 gatcggtgaa cttgcaggca taacccgtaa tgctgatcgc agtcagagtg gtaaggcata
     2701 tattaattat ctggaaaatg gagggctttt agaggctcaa ccgaaggagt ttacacaaca
     2761 agtgtttgat cctcaaaaag ggaccataga cctttcaaca ggtaatgtat caagtgtttt
     2821 gacatttata acaccaacat ttaccccagg agaagaagtt agagaaagaa aacagagtgg
     2881 taaatatgaa tatatgacat ttcttattgt aaatggtaag gatacatggt ctgtaaaagg
     2941 cataaaaaat cataaaggtg tatatgatta ttcaaaattg attcagtttg ttgaaaagga
     3001 taacaaacac tatcaggcga gaatgatttc tgaactcgga gataaagacg atgtggttta
     3061 ttctggagca ggctcatcag aagtatttgc tggtgaaggt catgataccg tatcttataa
     3121 taagacggat gttggtaaac taacaattga tgcaacagga gcatcaaaac ctggtgagta
     3181 tatagtttca aaaaatatgt atggtgacgt gaaggtattg caggaagtcg ttaaggaaca
     3241 ggaggtgtca gtagggaagc gaacagagaa aatacaatat cgtgattttg tattcagaac
     3301 cggtggaatt ccttatgatg taatagataa tcttcattct gttgaagagc tcattggcgg
     3361 aaaacatgat gatgaattca aaggcggtaa gtttaatgat atattccatg gcgcagatgg
     3421 gaacgattat atcgaaggta attatggtaa tgatcgacta tacggcgatg atggggatga
     3481 ttatatatcc ggaggacagg gagacgacca gttatttggt ggtagtggaa acgataaatt
     3541 gagtggaggg gatggtaata attatctgac aggaggaagc ggtaatgatg agcttcaggc
     3601 acacggagct tataatattc tgtcaggtgg tactggtgat gataaacttt atggtggtgg
     3661 tggtattgat cttctggatg gaggggaagg taatgactat ctgaatggtg gttttggtaa
     3721 tgatatttat gtttataggc aaaactatgg tcatcataca attgcagatg aaggaggtaa
     3781 aggagatcgt ttgcacttat ctgatattag ctttgatgat atcgcattta agagagttgg
     3841 aaatgatctt atcatgaata aagccattaa tggtgtactt tcatttaatg agtcaaatga
     3901 tgtcaatggg ataacattta aaaactggtt tgcgaaagat gcctcaggag cagataatca
     3961 tcttgttgag gttataacag ataaagatgg tcgagagata aaagttgata aaatacctca
     4021 taataataat gaacggtcag gttatataaa agccagtaat atagcatctg aaaaaagcat
     4081 ggttaatatc accagtgttg ccaatgatat taataagatt atttcttcag tttcagggtt
     4141 cgattcaggt gatgaacgat tagcatcttt atataattta tccttacatc aaaacaacac
     4201 acactcaaca actttaacga caactgtctg agactaaaag aggaggtgat ggccattcca
     4261 ggaggaatgg tcatcgttat atgatgagta aatgtagttc tcataatagt ctgtatgcac
     4321 tgatattgct tgcacaatat cataatataa ctgtcaatgc tgaaactata aggcatcagt
     4381 ataataccca cacacaagat tttggggtga ctgaatggtt actggcagcg aaatccattg
     4441 gcttaaaagc aaaatatgta gaaaaacatt tttccagatt gtcaataatt tctttacctg
     4501 cgttgatatg gcgggatgac ggtaagcatt atatattgtc tcgtgttact aaagattcat
     4561 cacgctatct tgtttatgat ccagaacaac atcagtcact aacttttagt cgggatgagt
     4621 ttgaaaaact gtatcaggga aaagtcattc tggttacgtc aagagcaaca gtagtcggag
     4681 agttagctaa atttgatttt tcttggttta tcccctctgt tgtgaaatac aggaggattt
     4741 tacttgaggt gttaactgtt tctgctttta ttcagtttct tgcgttaata acacctcttt
     4801 tttttcaggt tgtaatggat aaggttttag ttcaccgggg gttttcaacg ttaaatatta
     4861 tcacaatagc atttattata gtgatacttt ttgaagtgat attaaccgga gccagaactt
     4921 atattttctc tcatactaca agtcgtattg acgtcgaact gagcgctaag ttattcagac
     4981 atttgcttgc attgcctgtt tcatattttg aaaatcgcag ggtcggagag accgttgcca
     5041 gagtaaggga actggagcaa attcgtaatt ttttaaccgg acaagcgttg acatcagttc
     5101 ttgatctatt tttttctgta atattttttt gtgtcatgtg gtattacagc cctcaattaa
     5161 cactggttat attattgtca ctaccttgtt atgttatatg gtcattgttt atatcaccct
     5221 tattacgtcg acgtcttgat gataagtttc tcaggaatgc agaaaatcaa gcttttcttg
     5281 tcgaaacggt aacagcaata aatacaatta aatccatggc agtatcacca cagatgattg
     5341 ctacatggga taaacaactg gccgggtatg ttgcttccag tttcagggta aatctggttg
     5401 caatgacagg gcagcagggg atacagctga tacagaaaag tgtaatggta attagtctat
     5461 ggatgggagc acatcttgtc atatcgggag agataagcat cgggcagtta atcgcgttta
     5521 atatgcttgc aggtcaggtc attgctcctg ttatcagact ggctcatctt tggcaggatt
     5581 ttcagcaggt tggtatttct gttgagcgcc ttggtgatgt attaaataca ccggtagaga
     5641 aaaagtcagg cagaaatata ctgccggaaa ttcaggggga tatcgaattt aaaaatgtca
     5701 gatttcggta ttcttctgac ggtaatgtta ttttgaataa tattaattta tacatatcaa
     5761 agggggatgt tatcggtata gttggccgtt ctggttcagg aaaaagtaca ttaacaaaac
     5821 tgcttcagcg cttttatata ccagagaccg gacagatttt aattgatggg catgatttat
     5881 cacttgcaga tccagaatgg ttacgacgcc agattggtgt tgtattgcag gaaaatatac
     5941 tattaaatcg tagtattatc gataatatta cattagcttc tccggctgta tctatggaac
     6001 aggctattga ggcagccaga cttgcaggtg cccatgattt tattacagaa ctaaaagaag
     6061 ggtacaatac tattgttgga gaacagggcg ttggtctttc aggagggcag cgccaacgga
     6121 tcgcaatagc ccgggctctt gttacaaatc ctcgaattct tatttttgat gaagcaacca
     6181 gtgctcttga ttatgagtcg gaaaatataa taatgaaaaa tatgtcaaga atatgtaaga
     6241 acagaaccgt aattattatt gcacacaggt tgtcaactgt aaaaaatgca aacagaataa
     6301 ttgttatgga taatggcttt atttctgaag atggcacaca taaagagctt atctccaaaa
     6361 aagacagttt atatgcatat ttatatcagt tgcaggcata acgtatgcgc ttttacatga
     6421 aaggtttatg ggatttggtt tgccgttata agacggtttt ttctgatgtc tggaaaattc
     6481 gtcacacact tgatgctcct gtgagagaaa aagatgagta tgcatttctt cctgctcacc
     6541 ttgaactcat tgaaacacca gtatccagac gttctcattt tgttgtatgg agtattttat
     6601 tatttgtaat tatatctctt ctcttatctg ttcttgggaa agttgaagtg gtttcagtag
     6661 caaatggtaa gtttactcat agtggaagaa gcaaagaaat aaaaccgata gagaatgcga
     6721 ttgtagaaaa aataatggta aaagatggct cttttgtaaa aaaaaatgat ccattagttg
     6781 aattgacggt gcctggtgtt gaatctgata ttttaaaatc agaagcatct ttgttgtatg
     6841 aaaaaacaga acaatatcgc tacgcaattc tttctgaatc aatacaacgg aatgagcttc
     6901 ctgaaataag gataacagat tttcctggcg gagaagataa cgcgggaggt gaacattttc
     6961 agagggttag ctcattaata aaagagcaat tcatgacatg gcagaacaga aaaaatcaaa
     7021 agcagctcac attaaataaa aaaatagttg aacgggatgc agcgcttgct cgtgttagcc
     7081 tgtatgagca tcaggtatca caggaaggaa gaaaactcaa tgattttaag tatttgttga
     7141 ataaaaaagc tgtttctcaa cattcagtta tggagcagga aaatagctat attcaggcaa
     7201 aaaatgaaca tgcagtctgg cttgcacagg tttctcaact tgaaaaagaa atagaacttg
     7261 tgcgggaaga actggcactg gagacgaata tcttcagaag tgaaattatc gagaagcaca
     7321 gaaaatcaac agataacatt gtgttgctgg agcatgagct tgaaaaaaac aggcagagaa
     7381 aagcatcatc ttttattaaa gctcctgtga gtggtactgt tcaggagtta aatatacata
     7441 cagaaggtgg tgttgtcaca acagcagaaa cgctgatgat tattgtccct gataatgata
     7501 ttctcgaagt aacagcatct gtacttaaca aggatatcgg ttttatacaa cctggacagg
     7561 aggtcattat taaagtagat gcatatccat atacacgtca tggttacctt acagggaaag
     7621 taaaaaatat aactgcagat tctgtttctg ttccggatac agggcttgta tttaacgtga
     7681 ttatatctgt tgatcggaat gatatacagg gagaaagaaa aaaaatccct gttacagctg
     7741 gaatgactgt tatggcagaa ataaaaaccg gggtgcgtag tgttatcagc tatcttctta
     7801 gtccattaaa ggaaactatt aatgaaagtt tacgtgaacg ttagtatata aataacatat
     7861 tggcttttta tatgatatag atttctgaaa taatctcgta aagattatct atgttattta
     7921 aacaaactat gattgttctt gcccccttat ttccagacag gggttgtatc ttaagttaac
     7981 attacccgct gacgtcgata ttctcgcgga gagcgataac ccaacgcact atgcggatgg
     8041 ttttcattgt aatgctcgat cgccactgca aggttatgca atgccgttct tacattcggt
     8101 ttcggcatga acgcaatgta gtcttccttc atcgttttca cgaaccgttc tgccattcca
     8161 ttactctgag ggctactgat tgccgttgtg cagggcttca gattcagctc tctggcgaac
     8221 tgccgtgttt catgcgctct gtacgctgaa ccgttatctg tcagccactg gatggactgt
     8281 tccggtactt tatcgccaaa gcgtttttct atcgctccta acatcacatc ctgcactgtc
     8341 gctttatcgt aacctcccgt gcttgcggcc cagtctatgg cttcacgagc gcaacagtcc
     8401 aggacgaacg taacacgcag tttttcgcca tcatcgcaac cgaactcaaa gccatcggaa
     8461 caccaacgct ggtcgctttc cttcaccgat attcggcctt tatgttcccg ctgctgtcgt
     8521 gatggtttgt catgtaacaa taacaggtta ttttcgctca ttatccggta aacacgcttt
     8581 gcattcacga acggtagtcc ttcatttcgg gactgccgcc tgag
//
LOCUS       DAZGTR010000159         8614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGTR010000159 DAZGTR010000000
VERSION     DAZGTR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.161"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..360)
                     /locus_tag="ACHLCY_004325"
     CDS             complement(<1..360)
                     /locus_tag="ACHLCY_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFP0371442.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANWEMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPI"
     gene            complement(430..2124)
                     /gene="oxc"
                     /locus_tag="ACHLCY_004326"
     CDS             complement(430..2124)
                     /gene="oxc"
                     /locus_tag="ACHLCY_004326"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP0371443.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(2178..3428)
                     /gene="frc"
                     /locus_tag="ACHLCY_004327"
     CDS             complement(2178..3428)
                     /gene="frc"
                     /locus_tag="ACHLCY_004327"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP0371444.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(3941..4573)
                     /gene="yfdX"
                     /locus_tag="ACHLCY_004328"
     CDS             complement(3941..4573)
                     /gene="yfdX"
                     /locus_tag="ACHLCY_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP0371445.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            4869..5144
                     /gene="ypdI"
                     /locus_tag="ACHLCY_004329"
     CDS             4869..5144
                     /gene="ypdI"
                     /locus_tag="ACHLCY_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP0371446.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(5221..5463)
                     /gene="yfdY"
                     /locus_tag="ACHLCY_004330"
     CDS             complement(5221..5463)
                     /gene="yfdY"
                     /locus_tag="ACHLCY_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP0371447.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            5816..6736
                     /gene="lpxP"
                     /locus_tag="ACHLCY_004331"
     CDS             5816..6736
                     /gene="lpxP"
                     /locus_tag="ACHLCY_004331"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP0371448.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            7092..7163
                     /gene="ypdK"
                     /locus_tag="ACHLCY_004332"
     CDS             7092..7163
                     /gene="ypdK"
                     /locus_tag="ACHLCY_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP0371449.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(7228..8466)
                     /gene="alaC"
                     /locus_tag="ACHLCY_004333"
     CDS             complement(7228..8466)
                     /gene="alaC"
                     /locus_tag="ACHLCY_004333"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP0371450.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         2323 a         2069 c         1908 g         2314 t
ORIGIN      
        1 aataggatcg agaactgcaa accccaggaa tccaatggta ggtgaacccg caattaatgc
       61 acatacagct gcttctgcat gggtacgttt aaaaaatttg tagcaaccaa accaggagaa
      121 aaagaaacat ccgacaataa ccacaagtga tacaagggtc agacgagtgt ccgcaaaaat
      181 catttcccag tttgcccggg taatagaaac aaatagagcc gcaggaagag cgtagtttaa
      241 taccagttta ttaaatgcgc gggcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa
      301 ataacccaac agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat
      361 aatagtattt ccgataaggg tcccctcacc atcgcataat gagttggcaa gaggagacga
      421 tgtcagtatt taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc
      481 agttcctgct gcagggtcga tgaccacatt aataatggtc ggtttgcgcg actgaatacc
      541 ggtggttaaa gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa cgccacgaaa
      601 cgcatccatt aatttgtcat accttgcatg gtgcaacaga tccgttggtg atggtgcacc
      661 agcgccactg agatcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat
      721 cgtcaccggc aggttatatc gacaaatcgt ttcaatttcc atcccactga aaccaaaagc
      781 actatcacct tcaatggcga caaccggaga accagaagtc acgctagcac cgatggcata
      841 gcccataccg atgcccatga caccccaggt accacaatcc agacgacgac gtggtttata
      901 catatcaata atatttcgtg cattatccag ggtatttgca ccttcattaa ctaaataaat
      961 atcctggttc tcgcgcaata cgtcgcgcac agcacttaat gcattaaagt aatttaatgg
     1021 ttgagtatct gtacttaatt tttcatgcat tttttgtgca ttttgctgct tgtggatatt
     1081 taaaatatcg cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt ctgccagcat
     1141 accttgcata ctggatgcaa tatcaccaac gactggcaca gcaatggggc ggttgctgtc
     1201 aatttcctgc ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt
     1261 accgtgtgcc aataaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc
     1321 cagggcaaac gaacgcgcag ctgccgcaga aagtggatgc gtatcttcaa ggatcccttt
     1381 cgccatagac attggcagga atggaatctg agtactttca ataaattcac gaagctgttc
     1441 atcagcttgt gaatacgccg cgcctttgcc aaggataatt aatggccgtt cagcttttgc
     1501 taaaagcgaa attgcgctag tgactgactt cgggcatggc aataatgctg gcgacggatt
     1561 ttcaacttta acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt
     1621 tgctggcaaa tcaagataaa ctccgccagg gcgacccgat acagagaccc ggatagcgcg
     1681 tgccaatgca atgccaagat cctgcggctg attaacgcga aatgctgctt tggcatacgg
     1741 ttttgccgca ttcatttggt ccagctcttc ataatcacct tgctgtaggt cgacgatcgc
     1801 gcggtcgctg gagccgctaa tcatgatcat cggaaaacca tttaccgttg cgttggccaa
     1861 tgcggtcaaa ccattgagga atcctggcgc agaaactgtc aggcagatcc ccggtttttg
     1921 ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac tgctcatgac gaaaaccaat
     1981 ataacgaatg ccttccgcct gggcatggcg tgccatatcc gtcacaggaa tacctacaac
     2041 accataaata gtgtcaatat tattctgttt taatgcttca acgatgatat gcataccatc
     2101 tgtcatttga agttgatctg acatgattaa taacctcgaa atttttataa attgggagcc
     2161 agaagccccc gttaatatca gatggcgtgg ttttgcttca ttgcagcaat ttcatcgtcg
     2221 ctataaccca gctcctgcaa tacagcagcg gtatgttcac ctaatagcgg cgcagcttta
     2281 atatccggcg taaaggcaga gaatttcatt ggacaaccaa cggtcagata ttttccacgc
     2341 aacggttgtt ccacttcaac aacactgcca ctttggcgca aagagggatc aagtgaaatt
     2401 tctttcatac ttaaaaccgg tgcacaagga atatcaaact gagtcaaata ggccaccgct
     2461 tcatgtttat caatagtgac agtgtatttt tcgatttcag caaaaatatc gaaaatatgt
     2521 ggctgtcgtg catgggctgt actgtatgcc ggatcggtaa tccattctgg tttgccgatg
     2581 gctttacagg tgttttccca gttttgctcc tgaatagtga aataaatata ggcgttagga
     2641 tcggtttccc agcctttaca tttcaggatc cagccaggct gaccgccacc acctgcatta
     2701 ccaccgcggg gaactgcatc accaaatgta ccattcggat actgcgggta ttcttccaga
     2761 taacccaatt tatcgagacg ctgctggtca cgtaatttca cgcggcaaag gttcaatacg
     2821 gcatcctgca ttgacatggt gactcgttgc ccacgccccg ttttttcgcg atgcagcaaa
     2881 gcagcaagta aaccgatcag caaatgcatt ccggtgttgc tgtcacccaa cgctgcagcg
     2941 cttaccagcg gcggaccatc ccaaaaaccc gtagtggatg ccgcgccacc cgctgcctga
     3001 gcaacgtttt cataggcttt tacattcaca taaggcgaac actcatcaaa ccctttgatc
     3061 gaaccaaaaa tcagacgtgg attgatttct tgaatatgct cccaggtgaa gcccatgtga
     3121 tcaatggccc ctggatgaaa gttctcgact aagatatcag cttcgcggat cagcttttcc
     3181 attacctctt tgccttccgc tgttttggta tttaactcaa tagaacgttt gttactgtta
     3241 agcatggtga agtaaagcgc atcgatatca ggaatatctc gcagctggtg acgcgttacg
     3301 tcaccaacgc cgggacgttc aattttaata acgtcagcgc caaaccaggc cagcatttga
     3361 gtacaagatg ggccagattg cacaccggtg aaatcgagaa ctttaattcc ttgaagtgga
     3421 gttgacatag caatacctat tagttaatta aatatgtgtt aatttagcgt ttatttaagg
     3481 cgtaatatct tctataaata acccgcaaaa tatcgtcgta gctgcactcg atatttttaa
     3541 aatcctgtat caagtgctga aaatcatttc atcgccactt ccaattcctt tctgcatggt
     3601 aagtagtgag gacgaaaaga agaatatgat aaatattgat tcgtggtaat cagagagagt
     3661 cgcaacattt gtgatgttat tcttacaact gtaagaagcg ccggtgattt acaagtaagg
     3721 gtatgattac gctttttaaa gcgtaccata aaacataaca taataaatga tttgtgatga
     3781 cacgggatta ttgggattta ctttgaggaa gataaaatgc gtgtttatag aaccgcgagc
     3841 gccaaataat tttatgacca caacctgtcg tattgtggtc ataaaaaatt gtcggctgga
     3901 gggagttaaa tcctttacct caatatactg cctgtcagca ttagttcaca aacagtgctt
     3961 cgctgtcgac gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt
     4021 gctttttatc aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttaagcggca
     4081 tcagatattg gttttccatt acgccaacac cggccaggcg aagttcttcc attgcgccct
     4141 ttttatcccc tttcgccatt ttttcattgg caatttttat cgcggcttct ttttcgggag
     4201 ttgctacata actctcagaa attccgacag atgcattaat gacaatatat tggtcatcat
     4261 tgagattggt ctttttacct ggcttagcga actttgccca ttcggtactg tcatctgaca
     4321 gcaaagcgga agcttcattg gtcagttctt ttgccttttc tggatcgcca tgaaacagag
     4381 ccaggcgcgc tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg
     4441 ctgctgccgt tttctgggtt tgctgaactt gctgagtttg ctgttgtgcc gctaccggtg
     4501 cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa
     4561 ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc catcatattg
     4621 ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt atcttacagt
     4681 tgtagttatt atcgcaagaa gtattttagt cttacaattg taggaatctg cttcctgtcg
     4741 cagagaacta ttcttatgta ccacaaagag atgtcattta ttatatcgat gtaaatattg
     4801 attaattata tgtaaataca gccataacat cgtaggttct cagtgaacca ttttcaggat
     4861 aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc tctattatgg
     4921 catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc tataaagaga
     4981 ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca tcacaggcga
     5041 ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa atcacgccta
     5101 cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg tcacatggca
     5161 ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca tcctgaccca
     5221 ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg gggcaagaaa
     5281 cataaatatc cccgtcacta gttcattacc gccagttaac cacatggtga agtaataaat
     5341 gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca cgttcagtac
     5401 ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc ataaataaat
     5461 catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt aatagtaaga
     5521 caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcatg ttaaatgcaa
     5581 gactgttgtg tacggaaaaa tatttacttt gcacgattaa taatcagtag ctgaaagcag
     5641 tcagcgagtg ataaagtaca gataccgcat tcatgctcct cgtggttatg tcctgactag
     5701 tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat tgagtgtacc
     5761 gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg taactatgtt
     5821 tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca catggtttgg
     5881 gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct ttctcggcac
     5941 gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg cccgtaaaaa
     6001 cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga ttgccgaaaa
     6061 ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct ggcccgacag
     6121 tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg cacaaatgca
     6181 aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg gcggccgcgt
     6241 gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc agctgatgga
     6301 atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca gaaataatct
     6361 gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc ccgatcagga
     6421 ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg tcgccacaac
     6481 caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg taacgatggt
     6541 aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg aaggctaccc
     6601 gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag agatcatgcg
     6661 cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg tgggagaatc
     6721 gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg ccataataac
     6781 ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat ctaattatta
     6841 ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa agtaaataag
     6901 aaaatgtctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctc gagcagttta
     6961 gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt cgctttgccc
     7021 ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt aattacgcgg
     7081 agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc gtttgggccg
     7141 gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga gccatcaggc
     7201 tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat gtttgctgct
     7261 ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc ccacgaatcg cctgacgaat
     7321 acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt cgccaaagcc
     7381 aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa attccagcga
     7441 tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag ccttcggcat
     7501 ttcaaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc ggcgtttgta
     7561 ctgttcggca atgtcgcgca cgcactgttg atcgccctcc agcgccgcaa tcgccgccac
     7621 ctgcaacggc gtaaaggtgc cgtaatcgtg atagctttta atacgagcca gggcgctgac
     7681 cagcgttttg ttgccaacca taaagccgat acgccagccc gccatgttgt agcttttcga
     7741 cagcgtaaag aactcgactg ccacatcgcg tgcacccggt acctgcatga ttgacggcgc
     7801 tttccagcca tcgtagacga tatcggcata ggccaggtcg tggaccacca gcacatcgta
     7861 gcgtttcgcc agcgccacaa ccttttcgaa gaactccagc tccacgcatt gcgcggttgg
     7921 attcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac gaatggcgcg
     7981 ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta cctgcgcccc
     8041 ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca gcaccgtgtc
     8101 accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac caatagtgac
     8161 gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc agcgggaaat
     8221 ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt ccgggcgctg
     8281 ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg ggttacccat
     8341 gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt cagcggtgat
     8401 gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag ggcgagtgtc
     8461 agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg ctgccacaat
     8521 ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt agaacatttt
     8581 tatcaatggc tttaaatgag gaactccact tcaa
//
LOCUS       DAZGTR010000160         8588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGTR010000160 DAZGTR010000000
VERSION     DAZGTR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.162"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..105
                     /gene="rrf"
                     /locus_tag="ACHLCY_004334"
     rRNA            <1..105
                     /gene="rrf"
                     /locus_tag="ACHLCY_004334"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            299..375
                     /locus_tag="ACHLCY_004335"
     tRNA            299..375
                     /locus_tag="ACHLCY_004335"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:333..335,aa:Asp,seq:gtc)
     gene            537..1340
                     /gene="dkgB"
                     /locus_tag="ACHLCY_004336"
     CDS             537..1340
                     /gene="dkgB"
                     /locus_tag="ACHLCY_004336"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP0371451.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAVIGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(1337..2251)
                     /gene="yafC"
                     /locus_tag="ACHLCY_004337"
     CDS             complement(1337..2251)
                     /gene="yafC"
                     /locus_tag="ACHLCY_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP0371452.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIVRGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            2492..3292
                     /locus_tag="ACHLCY_004338"
     CDS             2492..3292
                     /locus_tag="ACHLCY_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0371453.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGLALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3370..4140
                     /locus_tag="ACHLCY_004339"
     CDS             3370..4140
                     /locus_tag="ACHLCY_004339"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0371454.1"
                     /translation="MTIQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALID
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(4188..5546)
                     /gene="mltD"
                     /locus_tag="ACHLCY_004340"
     CDS             complement(4188..5546)
                     /gene="mltD"
                     /locus_tag="ACHLCY_004340"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP0371455.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESSALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5618..6373)
                     /gene="gloB"
                     /locus_tag="ACHLCY_004341"
     CDS             complement(5618..6373)
                     /gene="gloB"
                     /locus_tag="ACHLCY_004341"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP0371456.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            6407..7129
                     /locus_tag="ACHLCY_004342"
     CDS             6407..7129
                     /locus_tag="ACHLCY_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0371457.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            complement(7126..7593)
                     /gene="rnhA"
                     /locus_tag="ACHLCY_004343"
     CDS             complement(7126..7593)
                     /gene="rnhA"
                     /locus_tag="ACHLCY_004343"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP0371458.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            7658..8389
                     /gene="dnaQ"
                     /locus_tag="ACHLCY_004344"
     CDS             7658..8389
                     /gene="dnaQ"
                     /locus_tag="ACHLCY_004344"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP0371459.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            8530..8588
                     /locus_tag="ACHLCY_004345"
     tRNA            8530..8588
                     /locus_tag="ACHLCY_004345"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8556..8558,aa:Asp,seq:gtc)
BASE COUNT         2165 a         2088 c         2076 g         2259 t
ORIGIN      
        1 tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga
       61 tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa attacgaaga
      121 atggcactga tctttgaggc agttcactac tcgataagag agtaagacaa aagcgaaagc
      181 ttttgaacgt tgcgaagcaa cggcccgaag ggtgaatcac gaagtgattc ataaactgcc
      241 aggcatcaaa ttaagcagta agccggtcat aaaaccggtg gttgtaaaag aattcggtgg
      301 agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg ggttcgagtc
      361 ccgtccgttc cgccacttat taagaagcct cgagttaacg ctcgaggttt ttttcgtctg
      421 gatatctatt attgccagaa tcgcaaaaat cctctgcatt ttacgctctt tttcctcaac
      481 agtctgaagc ccataatcac ctcagttaac gaaaatagca ttaaaagagg catattatgg
      541 ctatccctgc atttggttta ggtactttcc gtctgaaaga cgacgttgtt atttcatctg
      601 tgaaaacggc gcttgaactt ggttatcgcg caattgatac cgcacaaatc tatgataacg
      661 aagccgcagt aggtcaggcg attgcagaaa gtggcgtgcc acgtcatgaa ctctacatca
      721 ccactaaaat ctggattgaa aatctcagca aagacaaatt gatcccaagt ctgaaagaga
      781 gcctgcaaaa attgcgtacc gattatgttg atctgacgct aatccactgg ccgtcaccaa
      841 acgatgaagt ctctgttgaa gagtttatgc aggcgctgct ggaagccaaa aaacaagggc
      901 tgacgcgtga gatcggtatt tccaacttca cgatcccatt gatggaaaag gcgattgctg
      961 ttattggcgc tgaaaacatc gctactaacc agattgaact ctctccttat ctgcaaaacc
     1021 gtaaagtggt tgcctgggct aaacagcacg gcatccatat tacttcctat atgacgctgg
     1081 cttatgggaa agccttgaaa gatgaggtta ttgctcgtat cgcagctaaa cacaatgcga
     1141 ctccggcaca agtgattctg gcgtgggcta tgggggaagg ttactcagta attccttctt
     1201 ctactaaacg tgaaaacctg gaaagtaatc ttaaggcaca aaatttacag cttgatgccg
     1261 aagataaaaa agcgatcgcc gcactggatt gcaacgaccg cctggttagc ccggaaggtc
     1321 tggctcctga atgggattaa gcctctctga cagctcctcc gggagctgtt tttacatgct
     1381 cgctaaggaa atcgataaaa gcccggatgc gcgtacttac cgcacggtcg ctgtaataga
     1441 ctgcactgaa tggcatttcc actggcaaca ctttatctgc cattaactcc accaattctc
     1501 cgcgaacgat ttctttgtcg atcatgtagt cggacaaaca cgcaatcccg ttgccactca
     1561 ggcaaagctg tttcagtgtt tccccactat tggatgacaa accgtacttc acctcatgta
     1621 attgtccatc actacaggct atcggccagg tattgaggga agcgggttca gtgaatccca
     1681 ggcaaacatg ttgctttaaa tcgtcgatcg tttctggctt cccgtagcgg gaaatataat
     1741 cgggggaggc gataattttt cgataactgt taaataacgg cctggcacgt aagctggaat
     1801 ccgttaacgt accagcgcgt atcgcgacat ccacttttct ttcgatcaaa ttaataatcg
     1861 tttcggagga gactagcgat aaagtaactt ccggatagcg ttcacggaaa ggcttaatta
     1921 acggcatcag aaagtgcagc accactggag ttgcggcatc gatgcgtaac agtccacgcg
     1981 gtgtattacg cgtctccata atttctgatt ctgccgctgc catctcctgc aaaattgact
     2041 gtacgcgacg aaaataacgc tggccttctt ccgtcaggct aagttgtcgc gtggtccgat
     2101 taagcaggct aacgccaagt ttcatctcca gctttttcac tgcccggctt accgctgagt
     2161 ttgcttgccc taattgttcc gctgcccggc taaagctgcc gctttctacg accgaaacaa
     2221 aaatggcgag ttcttccgac gtggctttca tttttgctcc tgttgcaaaa tagaagagat
     2281 attttgaatt tatttgtcat taaaccatca ggatgcgtga tatgtcatcc gatttaatgt
     2341 tctcaataat gagcaaaatt ctgacctgtg taagcacttg cttacataac aatatacaat
     2401 tgctcgttga aagagtgagc taaaatccct ataacagtag aaccctcccg agtgcggaag
     2461 ggttgacgta atagaggttt caaagtcaaa agtgcgaaaa aacacctatg ccatgcgcta
     2521 tgttgccgga caacctgcgg aaaggatctt accgccgggg tcttttgcga gcatcggcct
     2581 ggcattacca cctggagaac cgttaagtac tgaagagcgt attcggatcc tggtgtggaa
     2641 catatacaaa cagcaacgcg ctgaatggtt gtcggtatta aagaactacg gcaaagatgc
     2701 acatctggtg ttattgcagg aagcgcagac aacgccagag ttagtacagt ttgcgaccgc
     2761 taactatctt gccgccgatc aggtgcccgc tttcgtgctg ccacaacatc cttccggtgt
     2821 aatgaccctt tcggcggcgc atccagtgta ttgctgcccg ttacgtgaac gagaacccat
     2881 tttgcgtctg gcgaagtcgg cactggtgac ggtctatcca ttacctgaca cccgcctgtt
     2941 gatggtggtt aatatacacg ccgtcaactt cagtctgggc gtggatgtct atagtaagca
     3001 gttacttcct attggtgatc agatagctca ccacagcggc ccggtcatta tggcgggaga
     3061 tttcaatgcc tggagccgta gaaggatgaa cgcgttatat cgctttgcgc gggaaatgtc
     3121 gctgcgccag gtgcgtttta ccgatgatca gcgccgtcgg gcgtttggtc gcccgctcga
     3181 ttttgttttc taccgtggtc tgaacgtcag cgaagcttct gtactggtta cgcgcgcttc
     3241 cgatcacaat ccgctactcg ttgaattcag tcccggcaag cctgataaat aaggtatgtc
     3301 aggtctgcca cagggcagac caacgtttgg cgctgccctt ttatactcaa ataacaaaag
     3361 gacagcgcaa tgacaataca atcccaccat gaccatgtag aaaagcagtt tagctctcaa
     3421 gccagcgaat atctgaccag taccgtacat gcatccggac gagatttaca gcgcctggcg
     3481 gtgcgtctgg ctgattatcc tggtgcaagt gtacttgata tgggctgcgg agcagggcat
     3541 gccagctttg tcgctgcgca aaacgtgagc acggtggtgg cgtatgactt atctgcccac
     3601 atgctagatg tcgtggcaca agctgccgaa gtccggcaac tgaaaaatat caccacccgc
     3661 cagggttatg ccgaaagtct gccatttgcc gacaacgcat ttgatattgt tatcagccgt
     3721 tattctgccc atcactggca tgatgttggt gcagcactgc gtgaagtgaa ccggatattg
     3781 aaacctggcg gtaggctgat tgtgatggac gtaatgtctc cgggtcaccc agtgcgcgac
     3841 atctggttac agacggtaga agcattacgc gatacctctc acgtacgaaa ctacgccagc
     3901 ggtgagtggt tgacgttaat caatgaagcc aatctgatag ttgataattt aattacagat
     3961 aagttaccgc tggaattttc ttcatgggtc gtgagaatgc gtacgccaga agcgttaata
     4021 gacgctattc gcatttacca acagagcgca tcgacagagg tgagaacgta ttttgccttg
     4081 cagaatgacg gctctttcac cagtgatatc atcatggtag aagcacataa agcggcataa
     4141 ataaaaaagg caccggggga atcggtgcct ttttattatc tggtttgtca ggaatctggc
     4201 atgttgttgt ttttcacaaa caacgtcagc ttatcgcctg gttgcagatt cgcagtgtcg
     4261 ctgttccagc gcatcacatc tttgatgttc acgccgtggc gtttagcaat gcttgaaagc
     4321 gaatcgcctt tgcgcacacg atacgtaatg ctatcgctgt tgtttgccag tcgctgtgcg
     4381 ctactgcctg cgccaatcgt caaactttgg cctggcttca gcttagatcc gcgcagtttg
     4441 ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatact tgaaagcgtg
     4501 tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgtcggc aaccagcgtc
     4561 gactgtacag cagcaatttc gcctgaagcc agagattcac gcagttgatc tgcatgcttc
     4621 tttggcacca tcacgtactg cggaccactt gcgcccagcg tggagccttt cacgccagcg
     4681 ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt cgccatttca
     4741 accgggctgc tcaggtgcac acgcgccaga gcactgcttt catcggtcgt tggcagacgt
     4801 acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt aggcacgtac
     4861 tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc acgcgctttg
     4921 ttcgttttaa ttgccttcat gactcgacct tcgccgctgt tataagccgc tacggtcaga
     4981 agccagtcgc cgtcaaacat cttgttcaga cgctgcatca tattcagcgc ggcagttgtt
     5041 gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc ataattgcgc
     5101 cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt tgcgtgagga
     5161 tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt acgtttttta
     5221 acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc gtggagatag
     5281 ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc cggaattccc
     5341 atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat agacgtccca
     5401 tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc agctgcagaa
     5461 aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag caggacagag
     5521 gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa gacgaccgat
     5581 cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct ttctttgacc
     5641 ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta attacattaa
     5701 ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt ttcagaatta
     5761 cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa taatcattta
     5821 tggacaaatc gtgcggaaga atactcaaag caaacttcat atttgataac gtatattcat
     5881 gagcacaaca taccaatgta tcgtcaggta acgcacttaa cttattaagt gattgataca
     5941 tttgtgatgc tgtcccttca aacaacctac cacacccacc agaaaacagc gtgtcgccgc
     6001 aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc ggtgtggcaa
     6061 ttacactaaa ttcatgcccc aaaacgaagg cagtttcgcc atctttgact acctgtgttg
     6121 ttcccttatc ttgtgtctct tgtggaccat acaccacaat ttgcggaaac ttttccacca
     6181 gttcttttac gccgccaacg tgatcgtgat gatggtgggt gagaaatatg gcctccggtt
     6241 gccagttatt ggcggcaatg gcgtttaata ctggctctgc gtctccggga tcgacaatca
     6301 ggcagcgacc tgcttcatca ttcaaaaccc agatgtaatt gtcatcaaag gcgggaatac
     6361 tgttaagatt catagattac ctcttagtgt gaaacggaag gttgtgatga aaccggcaag
     6421 agtccctcaa actgtcgtgg ctcctgatcg ctggggcgat ttgccctggg gagagcttta
     6481 tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg gttttcatct
     6541 gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg tttctcatca
     6601 agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac ttcatcttcc
     6661 ttttgccgat aaatccgttg atgtttgtct actggcacat acattgccgt ggtgcaccga
     6721 tccgcatcgt ttattgcgtg aaaccgatcg ggtattgatt gatgatggct ggctggtgat
     6781 tagtggcttc aatcccatta gtttaatggg attacgcaaa cttgtgccgg tattgcgaaa
     6841 aacctcgccc tataacagcc ggatgtttac tctgatgcgg caactggact ggctctcttt
     6901 gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga acaaacacgg
     6961 tggaaaacta ttgaatgcac atattcctgc gcttggttgc ttacaactta ttgttgcccg
     7021 gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca agccacgaat
     7081 tcgccaggcg gttggagcca cccggcaata tcgtaaacca caggcttaaa cttcgacttg
     7141 atagcctgta tcttccagtg tgggattcat tgccgcagca cgggccagtt catcacagcg
     7201 ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt gatgctgccc
     7261 caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt tgtctgctgt
     7321 tttccagcca cgttttttcc agttatggat ccactgggtg ataccttggc ggacatactg
     7381 gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg cgacaatagc
     7441 ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa aggttttctc
     7501 gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac ccagacacga
     7561 accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg taattgaatc
     7621 gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa ttacacgcca
     7681 gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcgcact atgaaggcca
     7741 caagatcatt gagattggtg ccgttgaagt ggtgaaccgt cgcctgacgg gcaataactt
     7801 ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg tacatggtat
     7861 tgccgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg agttcatgga
     7921 ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatcg gctttatgga
     7981 ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct gtaaggtcac
     8041 cgatagcctt gcggtggcga ggaaaatgtt tcctggtaag cgcaacagcc tcgatgcgtt
     8101 atgtgcccgc tacgaaatag ataacagtaa acgaacgcta cacggggcat tactcgatgc
     8161 acagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga tggcttttgc
     8221 gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca ttgtacgtca
     8281 ggcaagtaag ttacgcgttg tttttgcgac agatgaagag cttgcagctc atgaagcccg
     8341 tcttgatctg gtggagaaga agggcggaag ctgcctctgg cgggcataaa tacctgtaaa
     8401 aggcgctaaa aatagcggct tgggcgattt ttgcagcaaa cgattcaaaa gatgagaaaa
     8461 accgttgacg aagggtgagg caatccgtaa tattcgcctc gttcccaacg gaacacaacg
     8521 cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc gcgggttcga
     8581 gtcccgtc
//
LOCUS       DAZGTR010000161         8575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGTR010000161 DAZGTR010000000
VERSION     DAZGTR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.163"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..2642
                     /gene="cas3"
                     /locus_tag="ACHLCY_004346"
     CDS             <1..2642
                     /gene="cas3"
                     /locus_tag="ACHLCY_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP0371460.1"
                     /translation="WGKNRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCFGLADYF
                     RQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTL
                     GYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGQPPDRMDELDNFLPED
                     KAAARDFLLEIKPLFPLIESPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFP
                     RVAHPMDIKGYWQKTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDL
                     DISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLV
                     KTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFAD
                     SNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEG
                     LIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKS
                     DVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQ
                     LLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSV
                     DIDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDS
                     PGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPE
                     GFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLAT
                     CIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMAN
                     VILVNDDESCGYSATEGLIGKVG"
     gene            2740..4302
                     /gene="casA"
                     /locus_tag="ACHLCY_004347"
                     /gene_synonym="cse1"
     CDS             2740..4302
                     /gene="casA"
                     /locus_tag="ACHLCY_004347"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP0371461.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGTAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGADSPSFM
                     QDFEALKGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEHVCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWPNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTHDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRIPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLKKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLGKWQKEIWLFARQDFDERVFTNPYEPVDLKRVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4299..4838
                     /gene="casB"
                     /locus_tag="ACHLCY_004348"
                     /gene_synonym="cse2"
     CDS             4299..4838
                     /gene="casB"
                     /locus_tag="ACHLCY_004348"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP0371462.1"
                     /translation="MSIVKEEHKATLRKWREELQAKRGNRASLRRSTTVNDVCLSEGF
                     RSLLMQTHTLWKIEAQEWRFTALALVAAVSANVKAIDERQPFAAQLAAVMSKGRFTRL
                     SAVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIR
                     IRWALEYYQAGDADNEQNQ"
     gene            4850..5905
                     /gene="cas7e"
                     /locus_tag="ACHLCY_004349"
     CDS             4850..5905
                     /gene="cas7e"
                     /locus_tag="ACHLCY_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP0371463.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWSAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5916..6662
                     /gene="cas5e"
                     /locus_tag="ACHLCY_004350"
     CDS             5916..6662
                     /gene="cas5e"
                     /locus_tag="ACHLCY_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP0371464.1"
                     /translation="MSQYLIFQLHGPMASWGGDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGAWLSKEEPCTSQE"
     gene            6644..7294
                     /gene="cas6e"
                     /locus_tag="ACHLCY_004351"
     CDS             6644..7294
                     /gene="cas6e"
                     /locus_tag="ACHLCY_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP0371465.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7291..8211
                     /gene="cas1e"
                     /locus_tag="ACHLCY_004352"
     CDS             7291..8211
                     /gene="cas1e"
                     /locus_tag="ACHLCY_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP0371466.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8211..8504
                     /gene="cas2e"
                     /locus_tag="ACHLCY_004353"
     CDS             8211..8504
                     /gene="cas2e"
                     /locus_tag="ACHLCY_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP0371467.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
BASE COUNT         2117 a         2024 c         2378 g         2056 t
ORIGIN      
        1 tctggggaaa aaaccgacgc ggcgagaaag acgggggcga cgactatcac cttctttgct
       61 ggcattcgct ggatgtagcc gcgatgggct atttaatggt taaaagaaat tgcttcgggc
      121 tggctgatta ttttcgtcaa ttagggattt ctgacaagga acaggcggct caatttttcg
      181 cctggttgct gtgctggcac gatattggaa aatttgcccg ctcttttcaa caactttacc
      241 tgccccctga actcaagatt caggaaggtg ccagaaaaaa ttacgaaaaa atctctcatt
      301 caacgctggg ctactggttg tggaatcatt atttaagtga atgtcaggag ttacttcctt
      361 catcttcgct ctctccgcgc aaacttaggc gagtgataga aatgtggatg cccgtgacga
      421 cagggcatca tggtcagccc cctgatcgta tggatgaatt ggataatttt ctgcctgaag
      481 acaaagctgc agcacgagat tttctccttg aaatcaaacc actgtttccg ctcatagaga
      541 gccccgcatt ctgggatgat gatgagggca ttgaacttat aaaacacctt tcctggtata
      601 tctctgcaac cgtcgtgctc gcagactgga cgggttcgtc aacgcgattt tttccacgcg
      661 tcgcacatcc aatggatatt aaaggttact ggcagaaaac tttaattcag gctcaaaacg
      721 ccttaaccgt ctttcctcta aaagcaaaag tcgcgccttt caacggaatt aatacgctgt
      781 ttccttttat tgagaatccg acaccgttac agcaaaaggt gctggatctg gatatcagcc
      841 agcaaggacc gcagttattt attctggaag atgtgactgg cgcaggtaaa acagaagcgg
      901 cgcttatcct ggcgcacagg ttgatagctg cggggaaagc tcaggggctg ttttttggtc
      961 tgccaacaat ggcaacggcc aatgccatgt acgatcggct ggtcaaaacc tggcttgctt
     1021 tctattcgcc agaatcccgc cccagcctgg tgctggcaca tagtgcccgc acattaatgg
     1081 accgctttaa tgaatcactc tggtctggtg atttagtcgg gtcagaagaa ccggatgaac
     1141 aaacgttcag tcagggatgt gcggcctggt ttgccgacag taacaagaag gcgttgctgg
     1201 ctgaaattgg cgtcggcacg ctggatcagg cgatgatggc ggtgatgccg ttcaaacata
     1261 ataatctgcg gcttctgggg ctgagtaaca aaatcctgct ggctgatgag atccacgcct
     1321 gtgacgctta catgtcgtgc attcttgaag ggttgatcga gcggcaggcg cgtggcggaa
     1381 acagcgttat tttgctttct gccacgttat ctcaacagca gcgcgacaaa ctcgtcgctg
     1441 cctttgcgcg gggtacagag ggccagcaag aagctccgtt cctggaaaag gatgattacc
     1501 cctggctgac gcatgtcacg aaatccgatg tgaactcaca ccgggtagcg acgcgcaaag
     1561 atgttgagcg tagcgtcagc gtgggttggc ttcatagtga acaagagtgt attgcgcgta
     1621 tcgaatcggc ggtaagtcag gggaaatgca tcgcctggat ccggaattct gtcgatgacg
     1681 ctattaaggt tcatcgtcag ctgcttgccc gcggcgtcat tcctgcttcc agcctttcac
     1741 tttttcatag ccgctttgct tttagcgatc gccagcgaat tgaaacggaa acgctggcac
     1801 gctttggtaa atattgtagt ttacagagag cgtcgcaagt cattgtttgt acacaagtaa
     1861 ttgagcaaag cgtggatatt gacctggacg aaatgatctc cgacctggcc cctgttgatt
     1921 tgctgattca gcgagcagga cgattacagc ggcatatccg cgatattaat ggtcagttaa
     1981 agcgtgacgg aaaggacgag cgttcccccc ctgaattgct gattctggct cccgtctggg
     2041 acgactctcc tggcgacgaa tggtttggca gtgccatgcg taacagtgca tttgtctatc
     2101 ccgatcatgg gcgaatctgg ctgacgcagc gtgtgctgcg tgagcaaggt gctattcaga
     2161 tgccacacgc agcccggctt cttattgaat ccgtctacgg tgaggacgtg gtaatgccgg
     2221 aaggatttgc ccgcagcgag caggagcaag tgggcaaata ttactgcgat cgcgcaatgg
     2281 ctaaaaagtt tgtgctgaac ttcaggcccg gctatgccgc caatatcaac gattacctcc
     2341 cggaaaaact gtcgacacgt ctggctgagg agtctgtttc cctgtggctg gctacctgta
     2401 ttgacggtgt ggtgaagcct tatgccaccg gtgctcacgc atgggaaatg agcgttgtca
     2461 gagtgcgccg aagctggtgg aaaaaacatc gggatgagtt ttctttactg gagggagagg
     2521 cgttcaggct gtggtgcatt gaacagcgac aagatccgga gatggcaaac gtgattttag
     2581 tcaacgatga cgaaagttgc gggtattcgg ctacggaagg attgattggc aaggttggtt
     2641 gagatgtctg cgacaggact ctctgaatag ttgatacacc gaaaaggatg gaaagatgga
     2701 caaccgacct gggatggtct tttttacgag aggatgcgta tgaactcgtt ttcacttctg
     2761 acaaccccgt ggttgcctgt tcgttttaaa gacggaacga caggcaagct ggcaccagtc
     2821 gatctggctg atgaaaatgt tgtcgatatc gctgcgccgc gggcagatct ccagggtacg
     2881 gcctggcagt ttttgctggg gctactacaa accagcttcg cgcccaaaaa tcacggtcgt
     2941 tgggatgata tctggcaaga cggactggag gctgaaaagc tacgggaagc attgctgtcc
     3001 ttagaacacg ctttccagtt tggggcagat tccccttcat ttatgcagga tttcgaggcg
     3061 ctcaagggag ataaagttca ggtcgcttcg ctactgcctg agattcccgg cgctcaaaca
     3121 acgaagttta ataaagacca ctttattaag cgtggcgtga ctgaacacgt atgccctcat
     3181 tgttccgcgt tagctctgtt ctccctacag ttaaatgcgc catcaggcgg caaaggctat
     3241 cgcaccggtt tgcgcggcgg tgggccgatg accactctga ttgaattaca ggaatatcaa
     3301 ggcaatcaac aaacgccctt gtggcgcaaa ctgtggccca atgtgatgcc gcaggatgaa
     3361 gccgacttac cgctacccaa aaaatttgac gatctggttt tcccctggct tggcccgacg
     3421 cgaaccagcg aactggccgg tgcggtggta acccatgatc aggtcaataa actccaggct
     3481 tactggggaa tgccgcgccg tattcgtatt gattttaata ccacgacagt cggcaactgc
     3541 gatatttgcg gtgaacagag cgacgcgctc ctgagcctga tgacgaccaa aaattacggt
     3601 gcgaattatg ccatgtggca gcatccctta acgccttacc gtataccact taaagagggt
     3661 ggcgagtttt actcggtcaa accacaaccg ggcggtttaa tctggcgcga ctggttaggc
     3721 cttatcgaaa cgggtaaatc agaaaacaat acggaacttc ccgcgctggt ggtgaaactc
     3781 tttaatgcca gcagtctgaa acaggcaaaa gtaggcctgt ggggatttgg ctatgatttc
     3841 gacaacatga aagcgcgctg ttggtacgaa caccatttcc cgctgctgct caagaaaaaa
     3901 gaaggccaga taccgaaact gcgtctggct gcgcaaacgg cttcacggat tctaagtctg
     3961 ttacgaagtg cattgaaaga agcgtggttc tccgatccaa aaggtgcacg gggtgatttc
     4021 agttttgtgg atatcgactt ctggaacaaa actcagcatc gcttcctgag gttagtgcgc
     4081 caaattgaag aaggtcagga tgcggacgaa ttactcggca aatggcaaaa ggaaatttgg
     4141 ttattcgcgc gtcaggattt tgacgagcgt gtattcacca atccatatga gcctgttgat
     4201 ttgaaacgcg ttatgaccgc gcgcaagaaa tatttcacaa catcggcgga gaagcaaagt
     4261 gctaaagccg ccagggagaa aaagcaggag gctgctgaat gagtattgta aaagaggagc
     4321 acaaagccac gctgcgtaaa tggcgcgaag agttgcaggc taaacgcggc aatcgtgcaa
     4381 gtctgcggcg tagcacaacg gttaatgatg tttgcttgtc agaagggttt cgttctctgc
     4441 tgatgcagac ccacactttg tggaaaattg aagcacaaga gtggcgtttt accgcactgg
     4501 cgctggtcgc cgcagttagc gccaatgtaa aagctattga tgaacggcag ccttttgccg
     4561 cgcagctggc ggcggtaatg tcaaaggggc gttttactcg tctgtcggca gtaaaaacac
     4621 cggatgaatt actgcgccag ttacgccgcg cagtcagatt attaaacggt tccgtcaatc
     4681 ttgactctct ggcagagggg gtcttccgtt ggtgccagga gagcgacgac ctgctcaatc
     4741 atcaccgccg ccagcagcgg ccaaccgaat ttatccgtat ccgctgggcg ctggaatatt
     4801 accaggctgg cgacgccgat aacgaacaaa atcaataaag gactatctca tgacaacatt
     4861 tattcagctt catttgttaa ccgcttaccc tgctgccaac cttaaccgtg atgataccgg
     4921 tgcgccaaaa actgtggtgc tgggtggagc aacgcgtctg cgcgtttcct cgcaaagtct
     4981 gaaacgtgcg tggcgcactt ctgcactttt tgaacaggca ctggcgggcc atattggtat
     5041 tcgcagtggg cgtattgcgc gtgaggcggc aactatcctg attgagaaag ggattgaaga
     5101 taaaaaagcc atcgaatggt cggcaaaaat tgctgattat cttgggaaag ctaaaaatga
     5161 caaaaaacca aaagatccgc tcactaacgc cgaaactgaa cagttagtcc atatcagccc
     5221 ggcagaattt gacgccgtaa aagcgctggc ccatcagctt gcagaagaaa agcgcgcgcc
     5281 aaaagaggaa gatctcgcgt tgttacgcaa agaccgtatg gcggtagata ttgccatgtt
     5341 tggtcgtatg ctggcgaata aacccgagtt taatgttgaa gccgcctgcc aggtagcgca
     5401 cgcatttggt gtcagtgaaa cgattgtcga agatgatttt ttcaccgccg ttgatgattt
     5461 gcgccaggct tctgaagatg ccggtgccgg gcatcttggt gaaaccggat tcggttctgc
     5521 actgttctac acctatatct gcattgataa agatctgctg gtcgaaaacc ttggcggtga
     5581 cgaagcgtta gctaatcaga ccatacgtgc ctttacggaa gccgcactta aagtctcccc
     5641 aaccggcaaa cagaacagct ttgccagccg tgcctacgcc tcctgggcgc tggcagaaaa
     5701 aggcaccgat caaccacgct ctctggcggc ggctttctat gaacccatta atggcacacg
     5761 tcagttagat gtggcggtgc agcgtattac aacgcttcgc gaaaacatga atacggtcta
     5821 cgaacagaag accgaatgcg caagttttga cgtgatgaac aaacagggaa gcatgaagga
     5881 cgtgctggac tttatctgcg cgtaaggggc ggcttatgag ccaatatctg atttttcagc
     5941 ttcatgggcc gatggcgtcc tgggggggcg atgctccagg tgaagttcgt catacccatg
     6001 aacttccttc gcgctcagca ttgctggggc tactggctgc cggggtaggg attcggcgtg
     6061 atgataccga acgcttaaac gcgtttaatc gccactattc actggtggtt tgcgccagcc
     6121 gtaacccgcg ctgggcacgg gattatcaca cggtccagat gccaaaagag gtgcgtaaag
     6181 cacgttattt cagccgtcgc gaagagttga gcgaccctga gcttttgagc gcgattatct
     6241 cccggcgcga ctactacacc gatgcctggt ggatggtggc ggtggcaaca acagccgatg
     6301 cgccttacag cctcgaacag ttgcaggacg gtttacgcca tccagttttt ccgctttatc
     6361 tggggcgaaa aagtcatccg ctggcattac ctcttgcgcc gttactgctc gaaggcaacg
     6421 cgtgtgatgc cttatgtaat gcataccaac agtatcagga tcatttccat aaactgaaag
     6481 tctcactccc gaaacttcag gatgaatgct ggtgggaagg gaaacacgac ggcctggtcg
     6541 cgagcaaaat attacgccgc cgggatgttc ctttaaatcg tcagcagtgg ctgtttgggg
     6601 aacgcaccgt caatcagggg gcgtggctca gcaaggagga accatgtacc tctcaagaat
     6661 aaccttacat accggccagc tttcgcctgc gcagttgctg catctggtgg atcgcggtga
     6721 atatgtgatg catcagtggc tgtgggatct cttccctggc ggcaaagaaa ggcaatttct
     6781 ttatcgtcgg gaagaactcc agggcgcgtt tcgctttttt gtactgtcgc aggaacgccc
     6841 ggcggaaagt gatacgttca ccatagaatg ccggtcattt gcgcccgagc tgtgtaccgg
     6901 acaacaactt tgtttcaacc tgcgggcgaa tccaacaatc tgtaaagcgg gcaagcgcca
     6961 cgatctgctg atggaggcga aacggcaggt gagggggcag gcggaaggaa gtgatgtctg
     7021 gttgcatcaa caacaggcgg cgctggactg gctggcagcg cagggggaga gaagcggttt
     7081 tacactgctg gatacctcgg ttgatgccta ccgacaacag caactgcggc gggaaaactc
     7141 ccggcaactg atccagttca gtagcgtgga ttatacgggg atgcttacgg tcaccgatcc
     7201 tgggttattt gtgcaacgcc tgagccaggg gtatggtaaa agccgggcgt tcggctgtgg
     7261 tctgatgctg atcaaacccg gagcagaggc gtgacgtttg taccactgag cccgatccca
     7321 ttaaaagatc gcacctccat gatcttcctc cagtacggtc agatcgacgt actggacggc
     7381 gctttcgtgc tgatcgacaa aacagggatc cgaacgcata ttccggtggg atcggtcgcc
     7441 tgcattatgc tcgaaccagg aacgagagtt tcccacgcgg cggtgcatct ggccgccacg
     7501 gtgggaacac tgctggtctg ggtcggtgaa gcgggcgttc gcgtttactc ctccggacaa
     7561 cccggtgggg cgcgggcaga taaattactc taccaggcaa agctggcttt aacggaagat
     7621 ctacggttga aggtggtgcg caaaatgtat gaattacgtt ttcgtgaacc acagccagct
     7681 cgccgttcag tggagcagtt acgtggaatt gagggatctc gcgttcgcca gacgtatgca
     7741 ttactggcga aacaatatgg tgtgaaatgg aatggtcgca aatacgatcc taaagactgg
     7801 gaaaaaggcg atgttgtgaa tcgctgcatc agtgctgcta catcatgtct gtacggtata
     7861 tctgaagcgg cagtattagc cgcgggatat gcgcccgcta ttgggtttat ccatagtggc
     7921 aaaccgcttt catttgttta tgacatagcc gatatcatta aatttgattc ggttgtgcca
     7981 aaggcatttg aaatagcagc gaggcaaccc gcagaacctg ataaagaagt cagattagcc
     8041 tgtcgcgata ttttccgtag cactaagtta acgggcaaat taataccgtt aattgaggaa
     8101 gtccttgccg caggtgaaat tgaaccgcca caacctgcgc cggatatgtt accgccagcc
     8161 atccccgaac ctgaaacgtt gggcgatagc ggtcatcgag gacgcggctg atgagcatgg
     8221 tcgtggttgt tacagaaaat gtcccgccgc gcttacgtgg acggctcgca atctggctac
     8281 tggaagtgcg tgccggtgtg tatgttggtg atacatcaaa acgtattcgg gagatgatct
     8341 ggcagcaaat tacccaactg gctggttgcg gaaatgtggt gatggcctgg gcgaccaata
     8401 ccgagtcggg ttttgaattt cagacctggg gagaaaacag acgtattccg gtggatttgg
     8461 atgggttacg tttggtttct tttcttcctg ttgataatca ataagttatc cgttctttaa
     8521 aaataaggaa atgttttaat ttagttggta gattgttgat gcggaataaa tttgt
//
LOCUS       DAZGTR010000162         8521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGTR010000162 DAZGTR010000000
VERSION     DAZGTR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.164"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            307..1284
                     /gene="glaH"
                     /locus_tag="ACHLCY_004354"
     CDS             307..1284
                     /gene="glaH"
                     /locus_tag="ACHLCY_004354"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP0371468.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1304..2572
                     /gene="lhgO"
                     /locus_tag="ACHLCY_004355"
     CDS             1304..2572
                     /gene="lhgO"
                     /locus_tag="ACHLCY_004355"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP0371469.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2595..4043
                     /gene="gabD"
                     /locus_tag="ACHLCY_004356"
     CDS             2595..4043
                     /gene="gabD"
                     /locus_tag="ACHLCY_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0371470.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPMVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4057..5337
                     /gene="gabT"
                     /locus_tag="ACHLCY_004357"
     CDS             4057..5337
                     /gene="gabT"
                     /locus_tag="ACHLCY_004357"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP0371471.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5648..7048
                     /gene="gabP"
                     /locus_tag="ACHLCY_004358"
     CDS             5648..7048
                     /gene="gabP"
                     /locus_tag="ACHLCY_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP0371472.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVLALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7069..7731
                     /gene="csiR"
                     /locus_tag="ACHLCY_004359"
     CDS             7069..7731
                     /gene="csiR"
                     /locus_tag="ACHLCY_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP0371473.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWELRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7732..8181)
                     /gene="kbp"
                     /locus_tag="ACHLCY_004360"
     CDS             complement(7732..8181)
                     /gene="kbp"
                     /locus_tag="ACHLCY_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP0371474.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8265..8423)
                     /gene="yqaE"
                     /locus_tag="ACHLCY_004361"
     CDS             complement(8265..8423)
                     /gene="yqaE"
                     /locus_tag="ACHLCY_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0371475.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1921 a         2222 c         2376 g         2002 t
ORIGIN      
        1 gggcagataa tatgtttagt ttactaacgg tcattttgca gtgaagccat ttactgtttt
       61 ttatcgacca gataatctgt tctctaatgt taactccccc taacctgttg ctttagttat
      121 tcatttcctg tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt
      181 tgatcacaat aagaaaacaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt
      241 tttagattat ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg
      301 cttctgatga atgcactgac cgccgtacac aataacgctg tcgattcagg ccaggactat
      361 agcggattca ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc
      421 gaacagacga ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag
      481 tacaaatcgt ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag
      541 ctgcaaccat tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat
      601 gcggtgggta tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta
      661 gcgcatctga ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc
      721 gtggtgaaaa atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa
      781 ctgcacaacg acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc
      841 gatgagcaaa acatgcaggg tggaaattcg ttgctgctgc atctcgatga ctgggaacat
      901 ctggaccact atttccgcca tccactggcg cgtcgcccga tgcgctttgc cgcgccgccg
      961 agcaaaaacg tcagcaaaga tgttttccat ccagtgttcg acgttgatca acagggtcgc
     1021 ccggtgatgc gctatatcga ccagttcgtc cagccaaaag attttgaaga aggcgtgtgg
     1081 ttgagcgagc tttccgatgc cattgaaacc agcaaaggca ttctttctgt acccgttccc
     1141 gttggcaaat tcctgttgat aaacaacctg ttctggctgc acggtcgcga ccgctttact
     1201 ccgcacccgg atctgcgccg tgaactgatg cgtcagcgtg gctatttcgc ttacgccact
     1261 caccactacc agacgcatca gtaagcgcaa aggaattgag cggatgtatg attttgtgat
     1321 tattggcggc ggcatcatcg gcatgtcgac cgccatgcaa ctgattgatg tttatccgga
     1381 tgcccgcatt gcgttgctgg aaaaagagtc cggcccggcc tgtcaccaga cgggccacaa
     1441 cagcggcgtg atccatgccg gggtctatta cacgcccggc agcctgaagg cgcagttttg
     1501 cctggcggga aaccgcgcca ccaaagcctt ttgcgatcaa aacgacattc gctatgacaa
     1561 ctgcggcaag atgctggtcg ccacgtccga tctcgaaatg gaacggatgc gcgcgttgtg
     1621 ggaacgcacg gcggcgaacg gtatcgagcg cgagtggtta aacgccgagg aactgcgcga
     1681 gcgcgaaccg aatatcaccg ggctcggcgg catttttgtg ccgtccagcg gcattgtcag
     1741 ctatcgcgaa gtcacagcgg caatggcaaa aatcttccag gccagaggcg gtgagattat
     1801 ttataacgcc gaagtcagcg ccctcagtga gcataaaaac ggcgtggtcg tccgaacccg
     1861 tcagggcggc gaatatgaag catcgacgct gattagctgt tccgggctga tggctgaccg
     1921 gctggtgaaa atgctcggcc tcgaaccggg ttttattatc tgcccgttcc gtggcgagta
     1981 tttccgcctt gcgccggagc ataaccagat tgttaaccat ctgatttacc ccattcccga
     2041 cccggcaatg ccatttttgg gcgtgcatct cacccgaatg atcgatggca gcgtcaccgt
     2101 cgggccaaac gcggtgctgg ctttcaaacg cgaaggctat cgcaagcgcg acttctcatt
     2161 tagcgacacg ctggagattt tgggctcgtc ggggattcgc cgggtgctgc aaaaccatct
     2221 acgctcagga ctgggcgaga tgaaaaactc gctgtgcaaa agcggctatc tgcgcctggt
     2281 gcaaaagtat tgtccccggc tttcgttaag cgatctccag ccctggcccg ccggtgtgcg
     2341 ggcgcaggca gtatcgccgg acggcaagct gattgacgat tttctgtttg tcaccacccc
     2401 acgcacgatc cacacctgca atgcgccctc cccggcagcg acatcagcaa ttcctattgg
     2461 tgcgcatatt gtcagcaagg tacaaacgct gttggcaagc cagagtaacc ccggacgcac
     2521 gctgcgagcg gcacgtagtg tggatgcctt acacgccgca tttaatcaat aacctttgaa
     2581 aacaggacgt aacaatgaaa cttaacgaca gtaacttatt ccgccagcag gcgttgatta
     2641 acggggaatg gctggacgcc aacaatggtg aagtcatcga cgtcaccaat ccggcgaacg
     2701 gcgacaagct gggtagcgtg ccgaaaatgg gcgctgatga aacccgcgcc gctatcgacg
     2761 ccgccaaccg cgccctgccc gcctggcgtg cgctcaccgc caaagaacgc gccaacattc
     2821 tgcgcaactg gttcaatttg atgatggagc atcaggacga tttagcgcgc ctgatgaccc
     2881 tcgaacaggg caaaccactg gccgaagcga aaggtgaaat cagctacgcc gcctctttta
     2941 ttgagtggtt tgccgaagaa ggcaaacgca tttatggcga caccattccc ggtcatcagg
     3001 ccgataaacg cctgattgtt atcaagcagc cgattggcgt taccgcagct atcacgccgt
     3061 ggaacttccc ggcggcgatg attacccgta aagccggtcc ggcgctggcg gcaggctgca
     3121 ccatggtgct gaagcccgcc agtcagacac cgttctctgc gctggcgctg gcggagctgg
     3181 cgattcgcgc gggcattccg gctggcgtgt ttaacgtggt caccggttcg gcaggcgcgg
     3241 tcggtaatga actgaccagc aacccgatgg tacgcaaact gtcgtttacc ggttcgaccg
     3301 aaattggccg ccagttaatg gaacagtgcg cgaaagacat caaaaaagtg tcgctggagc
     3361 tgggcggcaa cgcgccgttt atcgtctttg acgatgccga tctcgataaa gccgtggaag
     3421 gcgcgctggc ctcgaaattc cgcaacgccg ggcaaacctg cgtctgcgcc aaccgtttat
     3481 acgtgcagga cggcgtgtat gaccgctttg ccgaaaaatt gcagcaggcg gtgagcaaac
     3541 tgcacatcgg cgacgggctg gataaaggcg tcaccatcgg gccgctgatc gatgaaaaag
     3601 cggtagcaaa agtggaagag catattgccg atgcgctgga gaaaggtgcg cgtgtggttt
     3661 gcggcggtaa agcggatgaa cgcggcggca acttcttcca gccgaccatt ctggtggacg
     3721 tgccggccaa cgccaaagtg tcgaaagaag agacgttcgg ccccctcgcc ccgctgttcc
     3781 gctttaaaga tgaagccgat gtgattgcgc aagccaatga caccgagttt ggccttgccg
     3841 cctatttcta cgcccgtgat ttaagccgcg tcttccgcgt gggcgaagcg ctggagtacg
     3901 gcatcgtcgg catcaatacc ggcattattt ccaatgaagt ggccccgttc ggcggcatca
     3961 aagcctcggg tctgggtcgt gaaggttcga agtatggcat cgaagattac ttagaaatca
     4021 aatatatgtg catcggtctt taactggaga atgcgaatga acagcaataa agagttaatg
     4081 cagcgccgca gtcaggcgat tccccgtggc gttgggcaaa ttcacccgat tttcgctgac
     4141 cgcgcggaaa actgccgggt gtgggacgtt gaaggccgtg agtatcttga tttcgcgggc
     4201 gggattgcgg tgctcaatac cgggcacctg catccgaagg tggtggccgc ggtggaagcg
     4261 cagttgaaaa aactgtcgca cacctgcttc caggtgctgg cttacgagcc gtatctggag
     4321 ctgtgcgaga ttatgaatca gaaggtgccg ggcgatttcg ccaagaaaac gctgctggtt
     4381 acgaccggtt ccgaagcggt ggaaaacgcg gtaaaaatcg cccgcgccgc caccaaacgt
     4441 agcggcacca tcgcttttag cggcgcgtat cacgggcgca cgcattacac gctggcgctg
     4501 accggcaagg tgaatccgta ctctgcgggc atggggctga tgccgggtca tgtttatcgc
     4561 gcgctttatc cttgcccgct gcacggcata agcgaggatg acgctatcgc cagcatccac
     4621 cggatcttca aaaatgatgc cgcgccggaa gatatcgccg ccatcgtgat tgagccggtt
     4681 cagggcgaag gcggtttcta cgccgcgtcg ccagccttta tgcagcgttt acgcgcgctg
     4741 tgtgacgagc acgggatcat gctgattgcc gatgaagtgc agagcggcgc ggggcgtacc
     4801 ggcacgctgt ttgcgatgga gcagatgggc gttgcgccgg atctcactac ctttgcgaaa
     4861 tcgatcgcgg gcggcttccc gctggcgggc gtcaccgggc gcgcggaagt gatggatgcc
     4921 gtcgctccag gcggtctggg cggcacctat gccggtaacc cgattgcctg cgtggcggcg
     4981 ctggaagtgt tgaaggtgtt tgagcaggaa aatctgctgc aaaaagccaa cgatctgggg
     5041 cagaagctaa aagacggatt gttggcgata gccgaaaaac acccggagat cggcgacgta
     5101 cgcgggctgg gagcgatgat tgccatcgag ctgtttgaag atggcgatca cagcaaaccg
     5161 gacgcaaaac tcaccgccga gatcgtggca cgcgcccgcg ataagggcct gattcttctc
     5221 tcctgcggcc cgtattacaa cgtgctgcgc atccttgtac cgctcaccat tgaagacgct
     5281 cagatccgtc agggtctgga gatcatcagc cagtgttttg ctgaggcgaa gcagtagcta
     5341 tgtccgtttg taagagttaa acgttgctaa gggaatgata acgatgcagt aaaaaataat
     5401 aacgatgagg tgatcgacaa agttaaaaac gactgcggac tgtcccctcg ctcctctggg
     5461 gagagggtta gggtgagggg taatcacgcg gctacaccct caccccggcc ctctccctaa
     5521 gggagaggga gaaaactctc ccgctttcgt ttttaagatt ttgttgaccg tctcaattaa
     5581 acgcgtgacc cgacgcggga aatgtcgggg cgctctccca agtgacacac tttcgagagg
     5641 attcaggatg gggcaatcat cgcaaccaca tgagttaggc ggcgggctga agtcacgcca
     5701 cgtcaccatg ttgtctattg ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt
     5761 cgccatcgcc gaagcgggcc cggcggtatt actggcctat ctgttcgccg gattactggt
     5821 ggttatgatt atgcggatgt tggcggaaat ggcagttgcc acgcccgata ccggttcgtt
     5881 ttccacctat gccgataaag ccattggccg ctgggcgggt tataccatcg gctggctgta
     5941 ctggtggttt tgggtactgg ttatcccgct ggaagccaac atcgccgcta tgatcctgca
     6001 ctcgtgggtt ccaggcattc ccatctggtt attttccctc gtcattaccc tcgccttaac
     6061 tggcagtaat ttattaagcg ttaaaaacta cggcgaattt gagttctggc tggcgctgtg
     6121 caaagtcatc gctatcctgg cctttatttt ccttggtgca gtcgcaatta gcggttttta
     6181 cccttatgcc gaagtgagcg ggatctcaag attgtgggat agcggcggct ttatgcccaa
     6241 cggcttcggt gcagtattaa gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga
     6301 aattgtcacc attgccgccg cggaatccga cacgccggaa aaacatattg tccgcgctac
     6361 caactcggtt atctggcgta tttctatctt ctatttgtgc tctatttttg tcgtattggc
     6421 gttaataccg tggaatatgc cggggctgaa agccgttggt tcttatcgct cggttctgga
     6481 attgctcaat attccccatg cgaaattaat catggactgc gtgatattac tttccgtaac
     6541 cagctgtctg aactcggcgc tgtataccgc gtcaaggatg ctctactcct taagccgtcg
     6601 cggtgatgcg cccgcgctaa tgggcaaaat caaccgcagt aaaaccccgt atgtggcggt
     6661 gttactctcc accggagcgg catttttaac ggtggtggtg aactattacg cacctgcgaa
     6721 agtgtttaaa ttcctgatag acagctccgg cgctatcgcc ctgctggttt atttagtcat
     6781 cgccgtttca cagttgcgga tgcgcaaaat tctgcgagca gaaggaagcg aaattcgctt
     6841 gcgcatgtgg ctttatccct ggctcacctg gctggtcatc ggctttatta cctttgtgtt
     6901 ggtagtgatg ctattccgtc cggcgcaaca gttagaagtg atctccaccg gcttattagc
     6961 gatagggatt atctgtaccg tgccaattat ggctcgctgg aaaaagctgg tattgtggca
     7021 aaaaacaccc gttcataata cgcgctgata atgatcagga gtcacaccat gaccattacg
     7081 tctctggatg gctatcgctg gctgaagaac gatattattc gcggtaattt tcaaccggat
     7141 gaaaaattac ggatgagttt gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg
     7201 gaagcacttt ctcaactggt ggcggaacgg ctggtcacgg tggtgaatca aaaagggtat
     7261 cgggtggcgt ctatgtcaga gcaggagctg ctcgatattt tcgacgcccg cgccaatatg
     7321 gaagcgatgc tggtcagtct ggcgattgcc cgcggtggcg atgaatggga ggcagacgtt
     7381 ctcgcaaaag cgcatctgct cagtaagctg gaagcctgtg acgccagcga gaaaatgctt
     7441 gatgagtggg agctgcgtca tcaggcgttt catacagcaa ttgtggcggg ctgtggttct
     7501 cactatttgc tgcaaatgcg tgaacggttg tttgatctgg cggcgcgcta tcgatttatc
     7561 tggctgcggc gaacggtgct ttcggtagaa atgctggagg ataaacacga tcagcaccag
     7621 accctgactg cggcggtact ggcgcgagat accgcgcgcg ccagtgagtt aatgcgccag
     7681 catttactga cgccaattcc cattatccag caggcgatgg ctggcaatta actactcttc
     7741 cggaatacgc aacacttgcc ccggataaat tttatccggg ctttttagca tcggtttatt
     7801 cgcttcgaag attttattgt acagattagc gttaccgtag acctgtttgg aaatggcact
     7861 cagagtgtcg ccagacttaa cggtataaaa ctggctggca gtggctggtg tcgccgtttt
     7921 cacctgatca tcgacactgg caataccgga aatattccca accgcaacaa ggattttctc
     7981 cttcgcctcc tgactcaggc cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat
     8041 gttcacttta tcggcatcag gtataccggt tttgctcaga tgctcctgca ccttcttcgc
     8101 ctgatcgtct ttatcgtgct gacctgtaac cgcgtcccag agtttttctc cggcatcttt
     8161 cacaaaattg aacagaccca tagctacctc attcgttaac ggaaacactc agaaagtgta
     8221 gcagaggaac gcggcgcgtt aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc
     8281 acccagaacg cgtgaatcaa accggggata tacccaagca gtgtcagcag aatattaata
     8341 atgaacgccc aaccgaaccc tttaccgagc agcacgccga gcggcggcag aatgatggtg
     8401 atgacgattc tccagaaacc catatgtact ccctataaga aaattactca ttgtttaaaa
     8461 agagatttta tctcttaagc gtagtaattt tagcggagcc tgccagtttc agcttacgct
     8521 t
//
LOCUS       DAZGTR010000163         8452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGTR010000163 DAZGTR010000000
VERSION     DAZGTR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.165"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..329
                     /locus_tag="ACHLCY_004362"
     CDS             <1..329
                     /locus_tag="ACHLCY_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371476.1"
                     /translation="SPLFIEIEDNLRTFNTNELTRKDNIFILFSSADDGPVMVVSSQR
                     LHDMLNPTKDTNWNSTCIYKSRHKILPINLTQETLFSSKSHGKYALFPIFTASWRATR
                     IKNIGI"
     gene            552..1049
                     /gene="ubiC"
                     /locus_tag="ACHLCY_004363"
     CDS             552..1049
                     /gene="ubiC"
                     /locus_tag="ACHLCY_004363"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP0371477.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1062..1934
                     /gene="ubiA"
                     /locus_tag="ACHLCY_004364"
     CDS             1062..1934
                     /gene="ubiA"
                     /locus_tag="ACHLCY_004364"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP0371478.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2089..4512)
                     /gene="plsB"
                     /locus_tag="ACHLCY_004365"
     CDS             complement(2089..4512)
                     /gene="plsB"
                     /locus_tag="ACHLCY_004365"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP0371479.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4683..5051
                     /gene="dgkA"
                     /locus_tag="ACHLCY_004366"
     CDS             4683..5051
                     /gene="dgkA"
                     /locus_tag="ACHLCY_004366"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0371480.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5161..5769
                     /gene="lexA"
                     /locus_tag="ACHLCY_004367"
     CDS             5161..5769
                     /gene="lexA"
                     /locus_tag="ACHLCY_004367"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP0371481.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5842..7167
                     /gene="dinF"
                     /locus_tag="ACHLCY_004368"
     CDS             5842..7167
                     /gene="dinF"
                     /locus_tag="ACHLCY_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP0371482.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7283..7492
                     /gene="yjbJ"
                     /locus_tag="ACHLCY_004369"
     CDS             7283..7492
                     /gene="yjbJ"
                     /locus_tag="ACHLCY_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP0371483.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7534..8049)
                     /gene="zur"
                     /locus_tag="ACHLCY_004370"
     CDS             complement(7534..8049)
                     /gene="zur"
                     /locus_tag="ACHLCY_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP0371484.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8367..>8452
                     /gene="yjbL"
                     /locus_tag="ACHLCY_004371"
     CDS             8367..>8452
                     /gene="yjbL"
                     /locus_tag="ACHLCY_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFP0371485.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIK"
BASE COUNT         1955 a         2036 c         2287 g         2174 t
ORIGIN      
        1 tttcacctct atttatagaa attgaagata atttaagaac ctttaacacg aatgaattaa
       61 cacgaaagga taatatcttt atcctgttct cctctgccga tgatgggcca gttatggtgg
      121 taagctccca gcgcttacat gatatgttga atcctacaaa agataccaat tggaattcca
      181 cgtgtatcta taaatccaga cataagatat tgcctattaa tcttactcag gaaacacttt
      241 ttagctccaa atctcatggt aaatatgcgc ttttcccaat ttttactgcg agttggcgag
      301 ctactcgtat aaagaatata ggtatttaag taaaggaaaa catcaccgtt cctggcatca
      361 tgggcggtga tgcccctacg gttgccctcg ccagcacggg catcggcaaa gcgtaaggtt
      421 caacagcggt ttaccacttc atgcgattgt tgcgtttttg ttgcgcatta gatcacttaa
      481 tttgctttac atctcccgta aacacttttc tgcgatacaa tgcctttacg ttatgtaacg
      541 gagagttcgg catgtcacac cccgcgttaa cgcaactgcg tgcgctgcgc tattttaaag
      601 agatccctgc cctggatccg caactgctcg actggctgtt gctggaggat tccatgacaa
      661 aacgttttga acagcaggga aaaacggtaa gcgtgacgat gatccgcgaa gggtttgtcg
      721 agcagaatga aatccccgaa gaactgccgc tgctgccgaa agagtctcgt tactggttac
      781 gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc cggtcgtacc gtcgttcctg
      841 tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt gggtaaaacg ccgttaggac
      901 gctatctgtt cacatcatcg acattaaccc gggactttat tgagataggc cgtgatgccg
      961 gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa accgctgttg ctaacagaac
     1021 tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa tatggagtgg agtctgacgc
     1081 agaataagct gctggcgttt catcgcttaa tgcgtacgga taagccaatt ggcgcgttac
     1141 tgctgctctg gccaacatta tgggcgttgt gggtggcgac accgggcgtt cctcagctct
     1201 ggatcctggc ggtatttgtc gcgggtgtct ggctgatgcg cgctgctgga tgtgtggtga
     1261 atgattatgc tgaccgaaag tttgatggtc atgttaagcg cacggcgaac cgaccacttc
     1321 ccagcggcgc ggtaacagag aaagaggcgc gcgcactgtt tgtagtgctg gtactgattt
     1381 cgtttttact ggtactgacg ctgaatacga tgaccattct gttgtcgatt gccgctctgg
     1441 cgttggcgtg ggtgtacccg tttatgaagc ggtataccca tctcccgcaa gtggtgctgg
     1501 gcgcggcgtt tggctggtcg attccaatgg cttttgccgc tgtgagtgag tcggtgccat
     1561 tgagttgctg gttaatgttc ctcgccaata ttctctgggc ggtggcttac gacacgcagt
     1621 atgcgatggt tgaccgcgat gatgatgtga agattggcat taaatccacg gcaatcctgt
     1681 tcggccaata cgataaattg attattggta ttttgcagat tggcgtactg gcactgatgg
     1741 cgatcatcgg tgagttaaat ggcttaggct ggggatatta ctggtcaatt gtggtggctg
     1801 gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg cgagcgtgaa gcctgcttta
     1861 aagcatttat gaataataac tatgttggtc tggtactatt tttagggctg gcaatgagtt
     1921 actggcattt ctgatgatgt aaaaaagccg gatgatcatc cggctttctt ctgggttgcc
     1981 tgatgcgcgg cgcttctcag gcctacacaa cacatcgcaa tttattgaat ttgcagatta
     2041 tggaaggccg gataaggcgt tttcgccgca tccggcaatt ctctctgatt acccttcgcc
     2101 ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta atcaactccg ccagcaactg
     2161 ataaaccttc atcgtttctg ccggttcggc atcgccgcta tcgctgatat acccttcatc
     2221 acgcagtgtc agcaccagag aactgaacac cgccttgtcg aagaactccg gcgcgttgat
     2281 gccgtgcagc acggagagac gttgcgcgac ggtgcggctc tctttctcca gcgtaccgcg
     2341 gttgatcgac gggttggcac tcaacaacca gaaggtgatg gcataacgtt gcagcgtttc
     2401 gcgcgcgcct gcggcgagca gctgtagcgt gcgagaatgc accgggttga tatgcaactc
     2461 atcatcttgc agggtaatca gcccctgacg ttgcatctca tttgccagcg catcaataac
     2521 gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc gctttcagca ttgggtaaag
     2581 cacattgacg tgctccatca atacgtcgcg ggagatgtgg cgatgctggg tgacaattgc
     2641 cgccatcagc gaaggcagca ccaacatatg cgcaatgttg ttgcgatagt aggtcatcag
     2701 caccgcttgc tcgcgtggca gaatgatgat gtcgccgata gtgtctttct cgacttcaaa
     2761 cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg ctggctgaag gaacggtaga
     2821 gtccgtggag taaggtacgt tgcgcatcag atccaggtag cagttgagtt gctcggttaa
     2881 ctgctcgcgg gtgagtgagc gctgacgtga tgccagtagc gcagtacagc acaggttcat
     2941 ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga tcggcagcaa tattattgac
     3001 cgtcggcgtt aaccaggccg gacgcaccgc ttcgatggga tcgatagatt cacgccagtc
     3061 tggtacgtgc tggttaaggt aggtcatcaa cggcattggt tcaccgaagt tgacgtaacc
     3121 ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc tgcggcaggc tctctttctc
     3181 tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact tccatgacgt gctcataacc
     3241 gatatagatc ggaatcagcg taatcggacg cgtgccgcca cgcagcatcg cctgaatggt
     3301 catcgacagc gtaccggttt tcggatccag caaacgcccc gtgcgggaac gaccgccttc
     3361 cacgaagtac tcgacggaat aaccacggct gaacagttcg ccgagatact cacggaaaac
     3421 ggtggaataa agtttattgc ctttaaaagt acggcgaata aagaacgccc ccagacggcg
     3481 gaaaatcggc ccggccggcc agaagttcag gttgatcccg gcggcgatat gcggcggcac
     3541 cagcccctgg tggtacagca cgtaagaaag tagcaggtag tccatgtgac tgcggtggca
     3601 aggcacatac accagctcat gaccgtcgtg ggccagctgg cgaacgcgct cggcgttatg
     3661 gacgttgatg ccctgataaa gtcggttcca ggtgaagccc agaatgcggt cagtcaggcg
     3721 gatcatctcg taagagaaat tcgccgcaat ctcttccatc agcgcaatcg cgttctgctg
     3781 cgctttttca tgggagattt ttttgctgcg cgcttcatct tctaccgctt tggcaatggc
     3841 gcgggaggcg agcagcttat taaacagatc ctgacgagca ggcagacgtg ggcccacagc
     3901 agccagacgt tgacgggcaa agtgcatacg cgccacgcgc gccagtttct gagcgatagt
     3961 tttatctgtg ccgtgttcat cggccatgcg gcgcagagaa actgacggcg agaaacgcac
     4021 aaaactgtcg cgaccgagcc acagtacggc gaaaaatttt tgtacgccgt taagcatacg
     4081 cagcggcggg tttacttcgc ctttttcacg ccccggcgcg cgaccaaaca tcaccgatac
     4141 tggcaccatc tgcacatcca gatttgggtt gctgcggtgc aaatcaagat agtcgtggaa
     4201 cagcttaata gactcttctt tcggcgtgta ataggtgaac acacgcggcc cgccgtgaat
     4261 gaacacatag cgcggcagta gcgtgccgtc gatttccagc ggctctaacg ggtcaggcaa
     4321 gtcatgagcc agacactggg cgcgcaacgt cagcaaatct gctttcgagt tgtacggtaa
     4381 aacgtacata attggacgag aggtatccag ccccagttcc ggggcaggat ctgccggaat
     4441 agacttgctt tttaccagga tgcttaatgg taaattcagt aatttgtagt aaattcgtgg
     4501 ccagccggac ataaacgatg taaagcctct ggttaataat gcaaatgcgc ggcaaggata
     4561 gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt tcgcgcggcg ttacgcaaac
     4621 ccgaatcatc ggatttaacg gtacactgat attgacgctc ataatgtaaa aaggttcttt
     4681 caatggccaa taataccact ggattcaccc gaattatcaa agctgctggc tattcctgga
     4741 aaggtttacg cgctgcatgg atcaacgaag cggcattccg tcaggaaggc gtagcggtat
     4801 tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc aattacccgc gtgctgctta
     4861 tcagctccgt gatgctggtg atgattgtgg aaatcctcaa tagcgccatc gaagcagtgg
     4921 ttgaccgaat tggctctgaa taccatgagc tttccggacg cgcaaaagat atgggatccg
     4981 ccgcggtgct gattgccatt atcgtcgccg tgattacctg gtgcatcctg ttatggtcgc
     5041 attttggata acccttccag atttcgataa atctctggtt tattgtgcag tttatggttc
     5101 caaaatcgcc ttttgctgta tatactcaca gcataactgt atatacaccc agggggcgga
     5161 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     5221 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     5281 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     5341 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     5401 cgtgtggctg ccggtgaacc gcttctggcg caacagcata ttgaaggtca ttatcaggtc
     5461 gatccttcct tgttcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     5521 aaagatatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
     5581 aacggtcagg tcgttgtcgc acgtattgat gacgaagtta ccgttaagcg cctgaaaaaa
     5641 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgttgac
     5701 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
     5761 tggctgtaac atatctctga gaccgcgatg ccgccaggcg tcgcggtttg tttttcatct
     5821 ctcttcatca ggcttgtctg catggcattc ctcacttcat ctgataaagc actctggcat
     5881 ctcgccttac ccatgatttt ctccaatatc accgttccgt tgctgggact ggtcgatacg
     5941 gcggtaattg gtcatcttga tagcccggtt tatttgggcg gcgtggcggt tggtgcaacg
     6001 gcgaccagct ttctctttat gctgttgctg tttttacgca tgagcaccac cgggctgact
     6061 gcgcaggctt atggtgccaa aaatcctcag gcattagccc gtgcgctggt gcaaccgttg
     6121 ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc gtacaccgat tatcgatctg
     6181 gcgctgcata ttgttggcgg tagtgaagcg gtcctggaac aggcgcggcg ctttcttgaa
     6241 atccgctggt taagcgctcc ggcgtcgctg gcgaatctgg tattactcgg ttggttactc
     6301 ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg tcggcaatat cctcaacatt
     6361 gtgctggatg tctggctggt gatggggctg catatgaacg tgcagggcgc ggcgctggcg
     6421 acggttattg cggaatatgc aacattgctg attggtctgc taatggtgcg taaaatcctc
     6481 aaactacgcg gaatttccgg cgaaatgctg aaaactgcct ggcgaggaaa cttccgtcgc
     6541 ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt tgttgcaact ctgtttcggc
     6601 gcgatcaccg tacttggtgc gcgactgggg agtgacattg tcgctgttaa cgcggttctg
     6661 atgacgctac tcacctttac cgcctatgcg cttgatggtt ttgcctacgc ggttgaagcg
     6721 cactccggtc aggcatacgg tgcgcgcgac ggtagccagt tgctggatgt atggcgggca
     6781 gcgtgccgcc agtcggggat tgtagcgtta ctgttttcgg tggtttattt gctggcaggg
     6841 gaacacatca ttgcgttgct gacgtcgtta acccagattc agcagctggc tgaccgctat
     6901 cttatctggc aggtgatttt gccgttggtt ggcgtctggt gttatctgct ggacggcatg
     6961 tttataggcg caacgcgtgc cgccgaaatg cgtaacagta tggcggtggc cgccgcaggt
     7021 tttgcgctga cgctccttac gctgccgtgg ctgggtaatc atggtttgtg gctggcatta
     7081 accgtctttc tggcgttgcg cgggctttct ctggcggcta tctggcggcg tcactggcgc
     7141 aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc tgaataaata atcctaagcc
     7201 aaattgctga ctacacttaa tctcacgttc agaagaaaag tgaacgtact ctcattcaca
     7261 acctaacgat gaggtcttga ttatgaataa agatgaagcc ggcggtaact ggaaacagtt
     7321 taaaggtaaa gtgaaagagc aatggggcaa actgaccgat gatgatatga cgatcattga
     7381 aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt tatggttatc agaaagatca
     7441 ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa tatcgctggt aattaatccc
     7501 tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc ggtttctttt tcacctgcac
     7561 agagtgatca tgctggcact gttcaggatg acgacacgct tccacttcca cgcatgccgc
     7621 acagagccca tgtgcttcaa tcacattatg ccgcagggca aaccccattt ttgccgccag
     7681 cgtatgcata atgtcttcca cgccttctgc acactcttct ttcaccgcgc cgcagcgatc
     7741 gcaaataaac atggctgacg tatgggtggg ctggtcgaac agatgacaga gcacataact
     7801 gttggtggat tccaccttat gcacaaaacc ttgctcaagc agaaaatcca gcgcgcgata
     7861 aaccgttggc ggcttggctt gcggttcagc ttcgcgcagt aaatcaagca gatcataagc
     7921 gctgatagcg ccatcctgca gactcatcag gcgcaacact tccaggcgct gtggggtcag
     7981 gcgcacatta cgctgcgcgc agattttttc agcctgcgct aataactcct gcgttgtggt
     8041 cttttccatc gggcacctca aagtgggggt taagaatgtc cttactttac catgttccag
     8101 gaaaaacacc gagaaccctt attgttgccg taatgttgat tttctgtttt gtaggtaagg
     8161 tgttgtgttg ccttgtcgta ccattatcaa cacgataata attaataatt catttttaaa
     8221 taaattgctt caagttaact cgctatttat ttaactgggt ttaaacgcat acctgtagtt
     8281 cagaaattat gagcgatttt tgatagtgac cagatggcaa aagaattata aggaggacac
     8341 ggtacgacaa aaaaggtaga tgaatcatgt tgaagataat acccggtgca actggatact
     8401 ttaataaaac actcaattca aatcaattcg ataatgaaga tgcaatcaaa ga
//
LOCUS       DAZGTR010000164         8288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGTR010000164 DAZGTR010000000
VERSION     DAZGTR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.166"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..665)
                     /locus_tag="ACHLCY_004372"
     CDS             complement(<1..665)
                     /locus_tag="ACHLCY_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004019902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371486.1"
                     /translation="MALYNITNKELHALEKTTFTLEGLQERYDLQEAIKKNIDIIAPD
                     CLVISEEFSDWEDSRRRIDLLAIDKQANLVVIELKRDETGAHMELQALRYAAMISTMS
                     FAKACEYFQTYLKKQNCDADAKEKILEFVELDETELVDFGKDIRIVLASSDFSKELTT
                     TAIWLRDKGVDIRCVRLTPYRFNDDVLINAEQIIPVPELEEYQVKFREKRDEQLISSQ
                     KKE"
     gene            962..1564
                     /locus_tag="ACHLCY_004373"
     CDS             962..1564
                     /locus_tag="ACHLCY_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HFP0371487.1"
                     /translation="MKNDKAWIGDLLGGPLMSRESRVIAELLLTDPDEQTWQEQIVGH
                     NILQASSPNTAKRYAATIRLRLNTLDKSAWTLIAEGSERERQQLLFVALTLHSPVVKD
                     FLAEVVNDLRRQFKEKLPGNSWNEFVNNQVRQHPVLASYSDSSIAKMGNNLVKALAEA
                     GYVDTPRRRNLQAVYLLPETQAVLQRLGQQDLISILEGKR"
     gene            1561..2163
                     /locus_tag="ACHLCY_004374"
     CDS             1561..2163
                     /locus_tag="ACHLCY_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1788 domain-containing protein"
                     /protein_id="HFP0371488.1"
                     /translation="MIDPVLEYRLSQIQSRINEDRFLKNNGSGNEIGFWIFDYPAQCE
                     LQVREHLKYLLRHLEKDHKFACLNVFQIIIDMLNERGLFERVCQQEVKVGTETLKKQL
                     AGPLNQKKIADFIAKKVDLAAQDFVILTGMGNAWPLVRGHELMSALQDVMGFTPLLMF
                     YPGTYSGYNLSPLTDTGSQNYYRAFRLVPDTGPAATLNPQ"
     gene            2175..5816
                     /gene="brxC"
                     /locus_tag="ACHLCY_004375"
     CDS             2175..5816
                     /gene="brxC"
                     /locus_tag="ACHLCY_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system P-loop protein BrxC"
                     /protein_id="HFP0371489.1"
                     /translation="MNIEQIFEKPLKRNINGVVKAEQTDDASAYIELDEYVITRELEN
                     HLRHFFESYVPATGPERIRMENKIGVWVSGFFGSGKSHFIKILSYLLSNRKVTHNGTE
                     RNAYSFFEDKIKDALFLADINKAVHYPTEVILFNIDSRANVDDKEDAILKVFLKVFNE
                     RIGYCADFPHIAHLERELDKRGQYETFKAAFADINGSRWEDERDAYYFISDDMAQALS
                     QATQQSLEASRQWVEQLDKNFPLDINNFCQWVKEWLDDNGKNILFMVDEVGQFIGKNT
                     QMMLKLQTITENLGVICGGRAWVIVTSQADINAAIGGMSSRDGQDFSKIQGRFSTRLQ
                     LSSSNTSEVIQKRLLVKTDEAKAALAKVWQEKGDILRNQLAFDPTTTTALRPFTSEEE
                     FVDNYPFVPWHYQILQKVFESIRTKGAAGKQLAMGERSQLEAFQTAAQQISAQGLDSL
                     VPFWRFYAAIESFLEPAVSRTITQACQNGILDEFDGNLLKTLFLIRYVETLKSTLDNL
                     VTLSIDRIDADKVELRRRVEKSLNTLERLMLIARVEDKYVFLTNEEKEIENEIRNVDV
                     DFSAINKKLASIIFDDILKSRKYRYPANKQDFDISRFLNGHPLDGAVLNDLVVKILTP
                     KDPTYSFYNSDATCRPYTSEGDGCILIRLPEEGRTWSDIDLVVQTEKFLKDNAGQRPE
                     QATLLSEKARENSNREKLLRVQLESLLAEADVWAIGERLPKKSSTPSNIVDEACRYVI
                     ENTFGKLKMLRPFNGDISREIHALLTVENDTELDLGNLEESNTDAMREVETWISMNIE
                     YNKPVYLRDILNHFARRPYGWPEDEVKLLVARLACKSKFSFSQQNNNVERKQAWELFN
                     NSRRHSELRLHKVRRHDEAQVRKAAQTMADIAQQPFNEREEPALVEHIRQVFEEWKQE
                     LNVFRAKAEGGNNPGKNEIESGLRLLNAILNEKEDFALIEKVSSLKDELLDFSEDRED
                     LVDFYRKQFATWQKLGAALNGSFKSNRSALEKDAAAVKALGELESIWQMPEPYKHLNR
                     ITPLIEQVQNVNHQLVEQHRQHALERIDARIEESRQRLLEAHATSELQNSVLLPMQKA
                     RKRAEVSQSIPEILAEQQETKALQMDADKKINLWIDELRKKQEAQLRAANEAKRAAES
                     EQTYVVVEKPVIQPVPKKTHLVNVASEMRNATGGEVLETTEQVEKALDTLRTTLLAAI
                     KAGDRIRLQ"
     gene            5862..>8288
                     /gene="pglX"
                     /locus_tag="ACHLCY_004376"
     CDS             5862..>8288
                     /gene="pglX"
                     /locus_tag="ACHLCY_004376"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system adenine-specific
                     DNA-methyltransferase PglX"
                     /protein_id="HFP0371490.1"
                     /translation="MNTNNIKKYAPQARNDFRDAVIQKLTTLGIAADKKGNLQIAEAE
                     TIGETVRYGQFDYPLSTLPRRERLVKRARELGFEVLVEHCAYTWFNRLCAIRYMELHG
                     YLDHGFRMLSHPETPAAFEVLDHVPEVAEALLPESKAQLVEMKLSGNQDEALYRELLL
                     GQCHALHHAMPFLFEAVDDEAELLLPDNLTRTDSILRGLVDDIPEEDWEQVEVIGWLY
                     QFYISEKKDAVIGKVVKSEDIPAATQLFTPNWIVQYLVQNSVGRQWLQTYPDSPLKDK
                     MEYYIEPAEQTPEVQAQLAAITPASIEPESIKVLDPACGSGHILIEAYNVLKNIYEER
                     GYRGRDIPQLILENNIFGLDIDDRAAQLSGFALLMMARQDDRRIFTRDVRLNIVSLQE
                     SLHLDIAKLWQQLNFHQQVQTGSMGDMFAENTALTQTDSAEYQLLMRTLKRFVNAKTL
                     GSLIQVPQEEEAELKVFLDALYRLEQKGDFQQKAAAKAFIPFIQQAWILAQRYDAVVA
                     NPPYMGGKGMNGELKEFAKNNFPDSKADLFAMFMQHAFSLLKENGFNAQVNMQSWMFL
                     SSYEALRGWLLNNKTFITMAHLGARAFGQISGEVVQTTVWVVNNNHTEFYRPVFFRLI
                     NGSEEEKKSALISRKNMFNHTLQNDFKKIPGEPIAYWVSDIYRDIFTQSPALSSIAKP
                     RQGLATSDNNRFLKLWYEPSLRKINFSAKDRADALNSQAKWFPCQKGGSYRKWFGNHE
                     YLVNWENDGRELLDFAASLYGSPTRTIKNIPFYFREGATWSTISSSDFSIRYSPTGFI
                     SETKGAVCFAD"
BASE COUNT         2309 a         1930 c         1977 g         2072 t
ORIGIN      
        1 ttctcttttt tctgactgct aatcagctgt tcatcacgtt tttcccgaaa tttaacttga
       61 tattcttcca attcaggaac cggtatgatt tgctctgcat tgataagaac atcatcatta
      121 aatctataag gcgtcagacg aacgcatcga atgtctaccc ccttatctct taaccaaatc
      181 gcagtggtag tcagttcctt gctaaaatcc gatgaagcca aaacaattct gatatctttt
      241 ccgaaatcaa caagctcagt ttcatccagc tcaacaaatt caagtatttt ttctttcgca
      301 tcggcatcgc agttttgttt tttcaaatac gtctggaaat attcacatgc tttcgcaaaa
      361 ctcattgtgg aaatcatcgc cgcatatcgt agtgcctgta actccatatg cgcacccgtt
      421 tcatcacgtt ttaattcgat aaccactaaa tttgcctgtt tatcgattgc caataaatca
      481 attcgccgac gactatcttc ccagtcagaa aactcttcgg aaattaccag acaatcagga
      541 gcaataatat ctatgttttt tttgattgct tcctgtaaat cataacgttc ctgtaatcct
      601 tccagagtaa aggtcgtttt ttctaaagca tgtagttctt tatttgtgat attgtacagc
      661 gccacgttta tcttcctttt ttaactaatt ttacagggac tttaacacag ttaaaatttt
      721 atttcctgag ctgtaatcga ttaagctgat gcattcaatg ggaatgatat agggtaattt
      781 ccagtctcac tcatagaaat ggctaaagca tgactctcgc caaaaccgtt tatgtgttgt
      841 acataacgcg atcatccctc tcacaaattg ccttttctca tggcatctcg cccagtcccc
      901 cattacaatc actttttgtt ttttgcgagc tgcattccag tcttcagagg gtttttcgat
      961 gattaaaaat gacaaggcat ggataggaga cttgctgggc gggccgctca tgagcaggga
     1021 aagccgcgtc attgccgagc tgttgctaac cgatcccgat gaacagacat ggcaagagca
     1081 aattgttggc cacaacattt tacaagcctc ttctcctaat accgcaaaac gttacgcggc
     1141 aacaatcagg cttcgcctga acacgcttga taaaagcgcg tggacattaa ttgccgaagg
     1201 tagcgagcgg gaacgccagc aacttctgtt tgtggctctg acgctacatt cgccggtagt
     1261 taaggatttt ctggctgaag tggtgaacga tctgcgcagg cagttcaagg aaaagttgcc
     1321 tggcaatagt tggaacgaat ttgttaataa ccaggttcgc caacatccgg tactcgccag
     1381 ctactcagat tcatctattg caaaaatggg aaacaatctg gtgaaggcgc ttgctgaagc
     1441 gggttatgtg gatacgcccc gcagacgtaa cctgcaggca gtttaccttt taccggaaac
     1501 tcaggcagtg ttacagcgcc tggggcaaca ggacttgata tctattctgg agggaaaacg
     1561 gtgatagatc ccgttcttga atatcgcctg tctcaaatcc agagtcgcat taacgaagat
     1621 cgcttcctca aaaataacgg ctccggaaat gaaattggtt tttggatctt tgattatccc
     1681 gcacagtgcg aactgcaggt acgggagcat ttgaaatatc tgctccggca tctggaaaag
     1741 gaccataaat ttgcctgtct gaatgtcttc caaatcatca tcgatatgct caatgaacgc
     1801 ggccttttcg agcgcgtctg ccagcaggaa gtcaaagtgg gtactgagac gctgaaaaag
     1861 cagcttgctg gtccgttaaa tcagaaaaag atcgctgatt ttatagcgaa aaaagtcgat
     1921 ctggctgccc aggattttgt cattcttacc ggcatgggca acgcctggcc attagtacgc
     1981 ggtcatgaac tgatgagtgc cttgcaggat gtcatggggt tcaccccact gctgatgttt
     2041 tatcctggca cctacagcgg gtacaacctt tccccgctca cagacaccgg ttcacaaaat
     2101 tattatcgcg ctttcagact ggtaccagat acgggacccg cagcaacatt gaatcctcaa
     2161 tgaagagcat aacaatgaat attgaacaga tttttgaaaa acctctaaaa cgaaatataa
     2221 acggggtagt caaagcagag caaaccgatg atgccagcgc gtacatcgag ttagatgaat
     2281 atgtcatcac ccgcgaactg gaaaaccatc ttcgccattt cttcgaatcc tatgttcctg
     2341 ccactggccc ggaaaggatc cgtatggaaa acaagatcgg cgtatgggtt tcaggcttct
     2401 tcggttcagg taaatcgcac tttattaaga ttctttctta tcttttatct aaccgcaaag
     2461 ttacacataa cggtacggaa cgtaatgctt actccttctt tgaagataaa atcaaagatg
     2521 cattattcct tgccgatatt aacaaagcgg tgcattaccc gactgaagtc attctgttca
     2581 atattgattc gcgtgccaac gtagatgaca aagaagatgc cattcttaaa gtcttcctga
     2641 aagttttcaa cgaacgcatt ggatactgcg ctgattttcc gcatattgcc catcttgagc
     2701 gcgagctgga taaacgcggt cagtatgaaa cctttaaagc cgcgtttgcc gatatcaatg
     2761 gctcacgctg ggaagacgag cgcgacgctt actacttcat cagcgatgac atggcacaag
     2821 cattaagcca ggccacgcag cagagtcttg aagcctcccg ccaatgggtg gaacaactcg
     2881 acaaaaactt cccgctggat atcaataatt tttgccagtg ggtaaaagag tggctggatg
     2941 acaatggtaa gaacatcctc tttatggtgg atgaagtcgg tcagttcatt ggcaaaaata
     3001 cgcaaatgat gctgaagctg cagactatta ctgaaaacct tggggtaatt tgcggtggcc
     3061 gcgcatgggt tatcgtgact tcgcaggccg atatcaacgc ggcaatcggt ggtatgagca
     3121 gtcgcgacgg acaggacttc tccaagatcc aggggcgctt ttcaacacgc ctgcaacttt
     3181 ccagctctaa cacatcagaa gttatccaga aacgtttgtt ggtaaagact gacgaagcaa
     3241 aagcagcgct ggcaaaagtg tggcaagaga aaggcgatat cctgcgtaac cagctggctt
     3301 ttgaccctac aacaactact gcactacgtc cttttaccag cgaagaagag ttcgttgaca
     3361 actacccgtt tgtcccgtgg cactatcaga ttctgcaaaa agtgtttgaa tctattcgga
     3421 cgaaaggtgc agcgggtaaa caattggcta tgggtgagcg ttctcagctg gaggcattcc
     3481 agactgcggc gcagcaaatc tcagcgcaag ggctggattc tctggtgcct ttctggcgct
     3541 tctatgccgc cattgagagc ttcctggaac ctgccgttag ccgcaccatc acccaggctt
     3601 gccagaatgg cattcttgat gagttcgatg gcaacctgct gaaaacgctg ttcctgatcc
     3661 gctatgtgga aacgctgaaa agcaccctgg ataacctggt cacattgtct atcgatagga
     3721 tcgatgccga taaagttgag ttgcgccgcc gggtcgaaaa aagtctgaac acgcttgaac
     3781 gcctgatgct cattgcgcgc gttgaagata aatatgtgtt cctgaccaac gaagaaaaag
     3841 agatcgaaaa cgagatccgt aacgttgatg tcgatttctc tgcgatcaac aaaaaactgg
     3901 catcgatcat ctttgatgac attctgaaaa gccgtaaata tcgttatccg gctaacaagc
     3961 aagactttga tatcagccgc ttcctgaacg ggcacccatt agacggcgca gtgcttaacg
     4021 atctggtggt gaagatcctg acccctaaag atccgaccta ttcgttctat aacagcgatg
     4081 cgacctgtcg cccttatacg tcagaaggcg acggctgtat tttgattcgt ctgccggaag
     4141 agggccgtac ctggagcgat attgatttag tcgtccagac tgaaaagttc ctcaaagata
     4201 acgccgggca acgtccggaa caggcaaccc tgctctcaga aaaagcgcgt gaaaacagca
     4261 accgggaaaa attactccgt gttcagttgg aatcactact tgcagaagca gatgtctggg
     4321 cgattggcga gcgcttaccg aaaaaatcct ccacgccatc gaatattgtc gatgaagcct
     4381 gccgttacgt gattgaaaac accttcggca agctgaagat gctgcggcct tttaacggtg
     4441 acatctcccg tgaaattcat gcattactga cggttgagaa cgacaccgaa ctggatctcg
     4501 gtaacctcga agagtccaac accgacgcca tgcgcgaggt agaaacctgg atcagcatga
     4561 atatcgaata caataaaccg gtgtatttac gcgatattct gaaccatttc gcgcgtcgcc
     4621 cttatggctg gcccgaagac gaagtgaaac tgctggtagc ccgtctggcc tgcaaaagta
     4681 aattcagctt cagccagcaa aacaacaacg tcgagcgaaa acaggcgtgg gagttattta
     4741 ataacagccg ccgccatagc gaattgcgtc tgcataaagt tcgccgtcat gatgaagcgc
     4801 aggtgcgtaa agccgcgcaa accatggctg acatcgctca gcagccgttt aacgaacggg
     4861 aagagccggc gctggttgaa catattcgtc aggtatttga agagtggaag caagagctga
     4921 acgtattccg cgccaaggca gagggcggaa acaatccggg gaaaaatgag attgaatccg
     4981 gtctgcgcct gcttaatgcc attcttaatg aaaaagaaga ttttgccctg atcgaaaaag
     5041 tctcatcgct gaaagatgaa cttctggatt tcagtgaaga ccgtgaagat ttggtcgact
     5101 tctaccgtaa gcaattcgcc acctggcaaa aactgggtgc tgcgctgaat ggcagcttta
     5161 aatctaaccg cagcgcgctg gaaaaagacg ccgcagcggt taaagcgctg ggcgagctgg
     5221 aaagcatctg gcaaatgccg gaaccttata agcatctcaa tcgcatcacg ccgttgattg
     5281 aacaggtcca gaacgtcaac catcagttag tcgaacagca ccgccagcac gccctcgaac
     5341 gcattgacgc ccgcattgag gaaagccgtc aacgcttgct ggaagcgcac gccacgtcag
     5401 aactgcaaaa cagcgttctg ctgccgatgc aaaaagccag aaaacgcgct gaggtcagcc
     5461 agtcgattcc ggaaattttg gcggaacagc aagagacaaa agcgctgcaa atggatgcag
     5521 ataaaaagat taacctgtgg atcgacgagc tgcgtaaaaa gcaagaggca caactccggg
     5581 cagcaaatga agccaaacgc gctgccgagt cagaacagac ttatgttgtg gtggaaaaac
     5641 ccgttatcca accggtaccg aaaaaaacgc atctggtgaa tgtcgccagt gagatgcgta
     5701 atgccaccgg tggtgaggtt ctggaaacca ccgaacaggt ggaaaaggcg ctcgacacgt
     5761 tacgcacaac gctgctggcc gccattaaag caggcgatcg cattcgcctt cagtaactcc
     5821 catttcaggg cagcactctg ctgccctttg caggattttc tatgaatacc aataacatca
     5881 aaaaatatgc cccacaggcc cgtaacgatt tccgcgatgc ggtgatccag aagctaacga
     5941 cgcttgggat cgctgcagat aaaaaaggca atctgcagat tgccgaggcc gaaaccattg
     6001 gcgagaccgt gcgttacggt cagtttgatt acccgttatc gacccttccc cgccgcgaac
     6061 ggctggtaaa acgcgcccgt gagctgggtt ttgaggtgct ggttgagcac tgcgcctaca
     6121 cctggtttaa ccgcttatgt gcaattcgct atatggagct gcacggttat cttgatcacg
     6181 gcttccgtat gttgtctcac ccggagacgc cggccgcgtt tgaggtgctg gatcatgtgc
     6241 cggaagtggc agaagccctg ctaccagaaa gtaaggcgca gttggttgaa atgaagcttt
     6301 ccggtaatca ggatgaagct ctgtaccgtg aactgctgct ggggcagtgc cacgccctgc
     6361 accacgcgat gccgttcctg tttgaagcgg tagatgacga agcggaactg ctgctgccgg
     6421 ataacctgac ccgtaccgac tccattctgc gtgggctggt tgatgatatt ccggaagaag
     6481 actgggagca ggtagaggtt atcggctggc tgtatcagtt ctatatttcg gaaaagaaag
     6541 atgctgtgat tggcaaagtg gtgaagagcg aagatattcc tgccgccacc cagctgttta
     6601 cgccgaactg gattgtgcag tatctggtac agaactccgt tggtcgccag tggttgcaaa
     6661 cctaccccga ctcgccgctg aaagacaaaa tggagtacta cattgagcct gcggaacaaa
     6721 cgccggaagt gcaggcgcag ctggcggcga ttaccccagc cagcattgaa cccgaaagca
     6781 ttaaagtgct cgacccggcc tgcggttccg gtcatatttt gattgaagcc tataatgtgc
     6841 tgaaaaatat ctacgaagag cgtggttatc gcgggcgtga tattccacaa ctgattctgg
     6901 aaaataatat ttttggtctt gatatcgacg accgagcggc acagctttcc ggctttgcat
     6961 tattaatgat ggcacgtcag gatgaccgca gaatatttac ccgcgatgta cgtctgaata
     7021 ttgtctcttt gcaggaaagc ctgcatctgg atatcgccaa actctggcag cagctcaatt
     7081 tccaccagca ggtgcaaacc ggcagtatgg gggatatgtt tgctgaaaat accgcgttaa
     7141 cccaaactga cagcgcagaa tatcagctgc tgatgcgcac gttgaaacgc tttgtgaatg
     7201 caaaaacgct gggctcactg attcaggtac cgcaggaaga agaagcggaa ctgaaggtat
     7261 ttctggacgc gctgtatcgc ctggaacaga aaggcgactt ccaacagaaa gcggcagcga
     7321 aggcgtttat tccgtttatt cagcaagcgt ggattctggc gcagcgatat gatgcggtgg
     7381 tggcaaatcc gccgtatatg ggcggcaaag ggatgaatgg cgagctgaaa gagtttgcca
     7441 aaaataattt cccggatagt aaagctgatt tgtttgcaat gtttatgcag catgcatttt
     7501 ctttacttaa agaaaatggg tttaatgcac aagtcaatat gcaatcatgg atgttcttgt
     7561 cgagctacga agcgcttcgc ggttggttat taaataataa aacatttatc acaatggctc
     7621 atttaggagc tcgagctttt gggcaaattt caggtgaggt tgtacagaca actgtatggg
     7681 tagttaataa taaccatact gagttttacc gccccgtatt tttccgctta atcaatggga
     7741 gtgaagagga aaaaaaaagt gcactaattt cgcgcaaaaa tatgtttaat cacaccttac
     7801 aaaacgactt caaaaaaatc cctggagagc caattgctta ttgggtaagt gatatatata
     7861 gggatatttt cacccagtca ccagctctct cctcaatagc taaaccaagg caagggcttg
     7921 caacttcaga taataataga tttttgaagc tatggtatga accatcatta agaaaaatta
     7981 acttttccgc aaaagatcgt gccgacgcat taaattctca ggcaaaatgg tttccttgcc
     8041 aaaaaggtgg gagttataga aaatggtttg gtaatcatga gtatcttgta aactgggaaa
     8101 atgatggccg tgaacttctt gattttgcag caagcctgta tggttctcca accagaacaa
     8161 taaaaaatat ccctttttat tttagagaag gtgcaacttg gtcgacaatt tcgtctagtg
     8221 atttttcaat tagatatagc ccaactggtt ttatatcaga aaccaaaggt gctgtttgtt
     8281 ttgctgat
//
LOCUS       DAZGTR010000165         8229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGTR010000165 DAZGTR010000000
VERSION     DAZGTR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.167"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            69..1793
                     /gene="iucA"
                     /locus_tag="ACHLCY_004377"
     CDS             69..1793
                     /gene="iucA"
                     /locus_tag="ACHLCY_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFP0371491.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1794..2741
                     /gene="iucB"
                     /locus_tag="ACHLCY_004378"
     CDS             1794..2741
                     /gene="iucB"
                     /locus_tag="ACHLCY_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFP0371492.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2741..4483
                     /gene="iucC"
                     /locus_tag="ACHLCY_004379"
     CDS             2741..4483
                     /gene="iucC"
                     /locus_tag="ACHLCY_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFP0371493.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            4480..5817
                     /gene="iucD"
                     /locus_tag="ACHLCY_004380"
     CDS             4480..5817
                     /gene="iucD"
                     /locus_tag="ACHLCY_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFP0371494.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHACEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            5823..8018
                     /gene="iutA"
                     /locus_tag="ACHLCY_004381"
     CDS             5823..8018
                     /gene="iutA"
                     /locus_tag="ACHLCY_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFP0371495.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1930 a         2108 c         2207 g         1984 t
ORIGIN      
        1 tctcattgat aatgagaatc attattgaca taattgttat tattttactg tgtgggagct
       61 gtttgactat gaccctgccc tctgaaaaac cagccacaga tgtggctgcg cagtgcttcc
      121 tgaatgcact gattcgtgaa accacagact ggaaactgac agaatacccg ccagacgaat
      181 tgatcatccc gctggatgag cagaaatcgc tccatttcag agtggcttat ttctccccaa
      241 cccagcatca ccgctttgca tttcctgcac gtctggtcac ggcatcaggc agttatcctg
      301 tcgactttac cactctctcc cggcttatta ttgataagct acggcatcaa ctgtttctgc
      361 ccgttcccct ctgcgaaact ttccaccagc gcgtgctgga aagccacgcc catacgcaac
      421 aggcaattga tgcccgtcat gactggaccg ccctgcgtga aaaagcgttg aattttggcg
      481 aagctgagca ggcgctgctg acaggacacg ctttccaccc tgcgcctaag tctcatgaac
      541 cgtttaaccg gcgggaggct gaacgctacc tgcctgatat ggcaccccac ttcccactgc
      601 ggtggttttc ggtggataaa acgcaaatcg ccggtgaaag tttgcatctc aaccttcaac
      661 agcggctgac gcgatttgcc gcagagaatg cgcctcagtt actcaacgaa ttaagtgaca
      721 accaatggct gttcccgttg cacccgtggc agggggaata tcttttgcag caggggtggt
      781 gccaggcact tgttgctaag gggctgatta aagacttagg tgaggccggc acgtcgtggc
      841 tgccgaccac ctcttcccgt tccctctact gtgccaccag ccgcgatatg atcaagttct
      901 ccctgagcgt acggctgacc aactccatcc gtactctgtc cgtgaaagaa gtgaagcgtg
      961 ggatgcgcct ggcacgcctg gctcaaaccg acggctggca gatgctacag gtccgcttcc
     1021 cgactttccg ggtaatgcag gaggatggct gggccgggct gctcgatctt aacggcaaca
     1081 tcatgcagga aagtctgttt gccctgcgtg aaaatctgct ggtggatcag ccgaaaagcc
     1141 agaccaacgt actggtctcc ctgactcagg ccgcacctga tggcggtgat tcgctgctgg
     1201 tttcggcggt aaaacgcctg agcgatcgcc tcggtatcac tgtgcaacag gccgcccatg
     1261 catgggtcga tgcatactgt cagcaagtgc taaagccgct gtttacggct gaagcggatt
     1321 acggcctggt gctgctggcg catcagcaaa atattcttgt ccagatgctt ggggatctgc
     1381 cggtcggatt tatttaccgt gactgtcagg gcagcgcttt tatgcctcac gcgacagact
     1441 ggctcgattc tattggcgag gcgcaggcgg aaaatatttt cacccatgag cagttgctgc
     1501 gctatttccc ttattacctg ctggttaact ccacttttgc tgtgaccgct gcgctggggg
     1561 ctgccgggct ggacagcgaa tcgaatctga tggctcgtgt acgagcatcg ctggctgaag
     1621 tgcgtgatca ggtgactcat aaaacctgtc tcaactacgt gctggaaagt ccgtactgga
     1681 acgtaaaagg taactttttc tgttatctga acgatcataa cgagaacacc attgttgacc
     1741 cttcggtgat ctatttcgat ttcgctaacc cgctgcaggc tcaggaggtc tgaatgtctg
     1801 aggcaaacat tattcacagc agatatggac tgcgctgtga aaaactcgac aagcccctga
     1861 atcttggctg gggactggac aatagcgcgg tgttgcactg tcccggggag ctgccgacag
     1921 ggtggctgtg cgatgcgctg gatcagatat ttatcgccgc accacaactg tcagcagtgg
     1981 ctctcccctg ggccgaatgg tgtgaggagc cacaggcgct gacgcttttt gggcaggtaa
     2041 aaagcgacat tatccatcgt accgccttct ggcagttacc gttatggttg agttcaccgg
     2101 caaaccgggc ctccggtgaa atggtttttg atgcagagcg tgagatttat ttcccgcagc
     2161 gcccaccccg tccgcagggt gaggtttacc gccgttacga tccacgtatt cgcaggatgc
     2221 tgagtttccg cattgccgat cccgtttctg atgcagaacg tttcacccgc tggatgaatg
     2281 atccgcgcgt tgagtatttc tgggagcaaa gtggctcact ggaggtacag accgcctatc
     2341 tggagcgcca gctcaccggt aaacatgcat tcccgctgat tgggtgcttc gacgatcggc
     2401 cattcagcta ttttgaaatc tactgggcgg cggaagaccg cattggtcgt cactattcgt
     2461 ggcagccctt tgaccgtggc ctgcatctgc tggttggtga acagcaatgg cgcggggccc
     2521 actatgtgca aagctggctg cgcgggctga cacattacct gctgctggat gagccccgta
     2581 cgcagcgcac agtactggag ccacgcaccg ataaccagcg cctgttccgc catcttgagc
     2641 ctgcgggata ccggacaatt aaagagttcg acttcccaca taagcgctcg cgcatggtga
     2701 tggcggatcg tcatcacttc ttcacggagg tcggtctgta atgaatcaca aggactggga
     2761 ttttgttaac cgtcggctgg tggcaaaaat gttgtctgag atggagtatg agcaggtttt
     2821 ccacgcggaa tctcagggcg atgaccacta ctgcattaac ctgccgggag cacaatggcg
     2881 ctttatcgct gaacgtggta tctggggctg gctgtggatt gatgcccaaa cgttgcgctg
     2941 cacggacgaa ccagtactgg cgcagacgct gctgatgcag ctaaagccgg tgctgtcaat
     3001 gagtgatgcc actgttgcag agcatatgca ggatttgtat gccacgctac tgggcgacct
     3061 gcaactgctg aaggcccgtc gcggactgag tgccagtgac ctgattaatc ttgatgccga
     3121 tcgtctgcaa tgcctgctga gcggtcatcc taaattcgtt tttaataaag gtcgccgtgg
     3181 ctggggcaaa gaggcgctgg aacgatatgc gccagagtat gccaacacct tcagactgca
     3241 ctggctggcg gtaaaacgtg aacatatgat ctggcgctgc gacaacgatc tggatattca
     3301 gcagttgttg actgccgcaa tggatccgca ggagtttaca cgcttcagtc aggtctggca
     3361 ggaaaacggg ctggatcaca actggctgcc gctgccggta catccgtggc agtggcagca
     3421 aaaaatcgcc accgacttca tcgctgattt tgccgaaggc aggatggtgt ctctcggcga
     3481 gtttggcgac cagtggctcg cccagcagtc gctgcgtacc ctgaccaacg ccagccgccg
     3541 gggagggctg gatatcaagc tgccgctgac catctacaac acctcatgct accgggggat
     3601 ccctggcaga tatatcgctg ccggaccact ggcttcacgc tggctgcaac aggtttttgc
     3661 gaccgacgcc accctggtgc aaagcggcgc agtgatcctt ggtgaaccgg ctgcaggcta
     3721 tgtgtcccat gaaggctatg ccgcgcttgc ccgggctccc tatcgctacc aggaaatgct
     3781 tggtgttatc tggcgggaga atccgtgccg ctggctgaaa ccggatgaaa gtccggttct
     3841 gatggcaaca ctgatggagt gcgacgaaaa caatcagccg ctggcaggcg catatatcga
     3901 tcgctccgga ctggacgctg aaacctggct tacgcaattg ttccgggtgg ttgtggttcc
     3961 gctgtatcac ctgctttgcc gctacggtgt cgcgcttatt gcacacggac aaaatataac
     4021 gctcgccatg aaagaggggg taccacagcg tgttctgctg aaagacttcc agggcgatat
     4081 gcggctggtg aaagaagcgt ttcccgaaat ggactctttg cctcaggagg tgcgtgatgt
     4141 cacatcccgt ctgagtgcgg actacttaat ccatgatttg cagacgggtc acttcgtgac
     4201 agtactgcgt tttatttcgc cactgatggt tcgtcttggc gtacctgaaa ggcgatttta
     4261 tcaactgctg gcagcagtgt tgagtgatta catgaacaaa catccacaaa tgtcagagcg
     4321 ttttgcgctt ttctcactct tcaggccaca aatcattcgc gtggtgctga acccggtaaa
     4381 actgacctgg ccggaccagg atggcggcag tcgcatgctg ccgaattacc ttgagaatct
     4441 gcaaaatccg ctgtggctgg taactcagga atatgaatca tgaaaaaaag tgtcgatttt
     4501 attggtgtcg ggacagggcc atttaatctc agtattgctg cattgtcaca tcagatcgaa
     4561 gaactgaact gtctcttctt tgacgaacat cctcattttt cctggcatcc gggtatgctg
     4621 gtaccggatt gtcatatgca gactgtcttt ctgaaagatc tggtcagtgc agttgcacct
     4681 acaaatccct acagctttgt taactatctg gtgaagcaca aaaagttcta tcgcttcctt
     4741 acaagcagac tacgtacagt atcccgtgaa gagttttctg actatctccg ctgggctgct
     4801 gaagatatga ataacctgta tttcagtcat accgttgaaa atattgattt cgacaaaaaa
     4861 agtcgattgt ttctggttca gaccagtcgg ggagaatatt ttgcccgcaa tatctgcctt
     4921 ggtacaggaa aacaacctta tttaccaccc tgtgtgaagc atgtgacaca atcctgtttc
     4981 catgcctgtg aaatgaatct tcgtcggccg gacctgagtg gaaaacggat aactgtggtt
     5041 ggtggaggac agagtggtgc agacctgttc cttaatgcat tacgcgggga atggggagaa
     5101 gcggcggaaa taaactgggt ctcacggcgt aataatttta acgcactgga tgaggctgct
     5161 tttgctgatg agtattttac acctgaatat atttcaggct tctccggact gaaggaagat
     5221 attcgccatc agttactgga tgagcagaaa atgacatcgg atggcatcac tgccgattct
     5281 ttactgacca tttatcgtga gttgtaccac cgttttgaag ttctgagaaa accaagaaat
     5341 atccgtctgc tacccagccg ctcggtaaca actctggaaa gtagtggtcc gggctggaag
     5401 ttattgatgg aacatcatct ggatcggggc agagagagcc tggaaagtga tgtggtgatt
     5461 ttcgccacag gttaccgttc tgcattgcca caaatacttc cctcactgat gcccttgatc
     5521 accatgcacg ataagaacac ctttaaagtg cgtgatgact tcactctgga atggagtggc
     5581 ccgaaagaaa ataacatctt tgcggtcaac gccagcatgc aaactcatgg catcgccgaa
     5641 ccccagctca gcctgatggc ctggagatct gcacgtattc ttaatcgcgt actggggcgt
     5701 gatttattcg atctcagtat gccgcccgca ctgattcagt ggcgcagcgg cagccggaaa
     5761 aaaccgcagc cggaggctgc tgctttaact cactatacaa caaatattca ggaataacga
     5821 tgatgcgcaa aaagtatatg ccccgggctc ttggtccgct gcttcttgtc gtgctgtcac
     5881 cagctgtcgc ccagcaaaac gatgataatg agatcatagt gtctgccagc cgcagcaatc
     5941 gaactgtagc ggagatggcg caaaccacct gggttatcga aaatgccgaa ctggagcagc
     6001 agattcaggg cggtaaagag ctgaaagacg cactggctca gttaatcccc ggccttgatg
     6061 tcagcagcca gagccgaacc aactacggta tgaacatgcg tggccgcccg ctggttgtcc
     6121 tgattgacgg tgtgcgcctc aactcttcac gttccgacag ccgacaactg gactctgtcg
     6181 atccttttaa tatcgaccat attgaagtga tctccggcgc gacggccctg tacggtggcg
     6241 ggagtaccgg agggttgatc aacatcgtga ccaaaaaagg ccagccggaa accatgatgg
     6301 agtttgaggc tggcacaaaa agtggcttta acagcagtaa agatcacgat gagcgcattg
     6361 ccggtgctgt ctccggcgga aatgaccata tctccggacg tctttccgtg gcatatcaga
     6421 aatttggcgg ctggtttgac ggtaacggcg atgccaccct gcttgataac acccagaccg
     6481 gcctgcagca ctccaatcgg ctggacatca tgggaaccgg tacgctgaac atcgatgaat
     6541 cccggcagct tcaactgata acgcagtact ataaaagtca gggggacgac aattacgggc
     6601 ttaatctcgg gaaaggcttt tccgccatca gcgggagcag cacaccatac gtcagtaagg
     6661 ggctgaattc tgaccgcatt cccggcactg agcggcattt gatcagcctg cagtactctg
     6721 acagtgattt cctgagacag gaactggtcg gtcaggttta ctaccgcgat gagtcgttgc
     6781 ggttctaccc gttcccgacg gtaaatgcga ataaacaggc gacggctttc tcctcgtcac
     6841 agcaggatac cgaccagtac ggcatgaaac tgactctgaa cagccaactt atggacggct
     6901 ggcaaatcac ctgggggctg gatgctgagc atgagcgctt tacctccaac cagatgttct
     6961 tcgatctggc tcaggcaagt gcttccggag ggctgaacaa ccataagatt tacaccaccg
     7021 ggcgctatcc gtcatatgac atcaccaatc tggcggcctt cctgcaatcc agctatgaca
     7081 ttaatgatat ttttaccgtc agcggtggcg tacgctatca gtatactgag aacagggtag
     7141 atgattttat cgactacacg cagcaacaga agattgctgc cgggaaggcg atatctgccg
     7201 acgccattcc tggtggttcg gtagattacg ataactttct gttcaatgct ggtctgctga
     7261 tgcacatcac cgaacgtcag caggcatggt tcaatttttc ccagggggtg gcattgccgg
     7321 atccggggaa atattatggt cgcggcatct atggtgcagc agtgaacggc catcttcccc
     7381 tgacaaagag cgtgaacgtc agcgacagta agctggaagg cgtgaaagtc gattcttatg
     7441 aactgggctg gcgctttacc ggtgacaacc tgcggactca aatcgcagca tattactcgc
     7501 tttccaataa gagcgtggaa aggaataaag atctgaccat cagtgtgaag gacgacaggc
     7561 gccgtattta cggcgtggaa ggtgcggtgg actacctgat cccggatact gactggagta
     7621 ccggtgtgaa cttcaatgtg ctgaaaaccg agtcgaaagt gaacggtcaa tggcaaaaat
     7681 atgacgtgaa ggaatcaagt ccatcgaaag cgacagctta cattagctgg gcgccggaac
     7741 cgtggagtct gcgtgtgcag agcaccactt ctttcgacgt aagcgatgca gagggtaacg
     7801 atattaatgg ttacactacc gtcgatttta tcagtagttg gctgcttccg gtgggaacac
     7861 tcagcttcag cgttgagaac ctcttcgacc gtgactatac cactgtctgg ggacagcgtg
     7921 cacctctgta ctacagcccg ggttacggcc ctgcttcact gtacgactac aaaggccggg
     7981 gccgaacctt tggtctgaac tactcagtgc tgttctgacg gggattcttt tgcaacaagg
     8041 gtatgctgta aacaacatgg tccttggtgg ccatgttgtt tacagccagt ataatataca
     8101 gccttaacaa gatattccgt ctccggaatg gactgttcac cactgaataa caatatgtct
     8161 gttttatgga tctttgactg aaatttatcg catatcctca tttcattgtc cgtctgaaaa
     8221 ctaacctta
//
LOCUS       DAZGTR010000166         8225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGTR010000166 DAZGTR010000000
VERSION     DAZGTR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.168"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..554
                     /locus_tag="ACHLCY_004382"
     CDS             <1..554
                     /locus_tag="ACHLCY_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0371496.1"
                     /translation="GESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRAN
                     SSLAMAISKGRLIRVNQGGKFRYCIPGADLPAEPEAASVAETDGKAFPQPAGVALPVQ
                     ETTTQEEMKTEIVEDIVKLQPSVTETKADDLILPSLHVANRELRRVKGQVQKWERVCA
                     ALRELNKCRDILRDITATREQQR"
     gene            587..868
                     /locus_tag="ACHLCY_004383"
     CDS             587..868
                     /locus_tag="ACHLCY_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0371497.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLRAVNNYVYFDRRVQA"
     gene            865..1161
                     /locus_tag="ACHLCY_004384"
     CDS             865..1161
                     /locus_tag="ACHLCY_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0371498.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAVYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            1158..1619
                     /locus_tag="ACHLCY_004385"
     CDS             1158..1619
                     /locus_tag="ACHLCY_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0371499.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERQVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRR"
     gene            1597..1953
                     /locus_tag="ACHLCY_004386"
     CDS             1597..1953
                     /locus_tag="ACHLCY_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371500.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGSVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRASKSRTE"
     gene            2049..2420
                     /locus_tag="ACHLCY_004387"
     CDS             2049..2420
                     /locus_tag="ACHLCY_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371501.1"
                     /translation="MTELTKEQLIKEAKLKIAVANCYPNSEMARVEGELFKIALASLE
                     AEPIAWECGENIILFNPDTVEAYAKRAEISPKPLFSAPPALVVPDKLPREYRNGWPLA
                     YSDYAEGWNDCREAMLQGDKS"
     gene            2417..2770
                     /locus_tag="ACHLCY_004388"
     CDS             2417..2770
                     /locus_tag="ACHLCY_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0371502.1"
                     /translation="MINRIKLEHILEYARQQRHIGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTPDGWISCSERMPPVGVDVQLFVSSTGEQFTGFNLDNTGDFQYAQCFGAPIVCEA
                     THWMPLPEPPKEVRQ"
     gene            complement(2976..3275)
                     /locus_tag="ACHLCY_004389"
     CDS             complement(2976..3275)
                     /locus_tag="ACHLCY_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0371503.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGSVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGIYHGGQSYETDFL"
     gene            complement(3281..3538)
                     /locus_tag="ACHLCY_004390"
     CDS             complement(3281..3538)
                     /locus_tag="ACHLCY_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0371504.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRAGK"
     gene            3674..3952
                     /locus_tag="ACHLCY_004391"
     CDS             3674..3952
                     /locus_tag="ACHLCY_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371505.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFYRRHWIISVRLPYSECPPRPSRTFQQRIWR"
     gene            3954..5003
                     /locus_tag="ACHLCY_004392"
     CDS             3954..5003
                     /locus_tag="ACHLCY_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0371506.1"
                     /translation="MRVLLRPVLVPELGMVVLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGVGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQVEL
                     IFRFLDHAFATGVLG"
     gene            5016..5390
                     /locus_tag="ACHLCY_004393"
     CDS             5016..5390
                     /locus_tag="ACHLCY_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0371507.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGK
                     PVSGGRLGVKIYPIMLEGQVKK"
     gene            5387..6208
                     /locus_tag="ACHLCY_004394"
     CDS             5387..6208
                     /locus_tag="ACHLCY_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0371508.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            6435..6632
                     /locus_tag="ACHLCY_004395"
     CDS             6435..6632
                     /locus_tag="ACHLCY_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0371509.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            6783..7841
                     /locus_tag="ACHLCY_004396"
     CDS             6783..7841
                     /locus_tag="ACHLCY_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0371510.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWLLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            7887..7957
                     /locus_tag="ACHLCY_004397"
     tRNA            7887..7957
                     /locus_tag="ACHLCY_004397"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7915..7917,aa:Met,seq:cat)
     gene            complement(8061..8195)
                     /locus_tag="ACHLCY_004398"
                     /pseudo
     CDS             complement(8061..8195)
                     /locus_tag="ACHLCY_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2150 a         1856 c         2331 g         1888 t
ORIGIN      
        1 caggtgaaag tgaaccagtg tcaccggtac cgaaagtgac agcgcaaatg ctgactgagg
       61 cgattgaaca acatggccca caaacggcgg atgagctggc actgatgttc gggattacct
      121 cccgccgggc gaattcatcg ctggccatgg caatcagcaa agggcgtctg attcgcgtga
      181 atcagggcgg taaatttcgt tactgcatac cgggcgctga tttaccggca gagccggaag
      241 ctgcatccgt agcggaaacc gatggtaaag cctttcctca gccagcaggt gttgcgttac
      301 cagtccagga aacgacgaca caggaagaaa tgaaaacaga aatcgtggaa gacattgtga
      361 agttacagcc atcggtcacc gaaacgaaag cagatgacct gattctacca tcgctgcatg
      421 tggctaaccg cgagctgcgc cgggtaaaag gtcaggttca gaagtgggag cgagtctgcg
      481 ccgcgctgcg ggagctgaac aagtgccggg atattctccg ggatattacc gccaccagag
      541 aacagcagcg gtgagtgggt ggaagacgtg gtgccgggct gaaattctga tactccggca
      601 gtgcgcggga acaatgaagg taaaaagcat tggcgcgctt atcggacgaa ctgaagcggc
      661 agtgagaacg aaggcacggg agctgggcat cagcatgatg ttacgtggtg attttcaccc
      721 gtcggcaaaa tattcacagc gtgatattga gctggcgcgg caactgcatc agcgcggtgt
      781 accccgacgg gaaattgccg aaaagttcgg aatgaagttg cgtgcagtga ataactacgt
      841 ttatttcgac aggagggttc aggcgtgagg gtgagaattt atatcgccgg tccaatgacg
      901 ggatatgaaa atttcaaccg cgaggcattt cacagggcgg aagaagcact gaaacgggaa
      961 gggcataccg tcttaaaccc ggcagtactt ccggacgggc tgacacagcc gcactacatg
     1021 gatatttgca tggcaatgat tcgttgtgtg gatgcggttt atatgctgaa tggctggcag
     1081 cggtcagcgg gcgctaaggc agagctggca ctggcggaga aactggggca tgcggtgatt
     1141 tatcaggagg tggctcaatg agagaggtta actatgaggc gcttcgtgag gcagcacaaa
     1201 actatcagtc gacgctggcg tggtatcagg ctatcccgga cagcccaaat gctgaacggg
     1261 attgtgatgc ggctcttgct gcgtttaagc gtcacatccg tcatcgggaa gcggatatta
     1321 tcgctgattt gctggatgga ctggaagaag caaaatcaca actcaacgag caacgtgagt
     1381 attacgaagg tgttatctcg gatgggagta agcgcattgc tgaactggaa gcgcgggaag
     1441 ttcaattacc gactcgctac gaccttcgat atggacaccc gataaatgca gatgagcgac
     1501 aagtcatgat acctaaagaa aatggcagtt ggctttacct gattgaccta gaacacgcat
     1561 tacgcgtcgc tggcattcgc atcaaaggag aggagcatgg aaataaaacc agaagatgag
     1621 ttaagtaata ttgttttatt tccggtaaaa gaggatgacc cacgtaatca ggttaatttt
     1681 ctttatgagc catcggaaag accatactgc catcacgcct ctgtccgggt tgacgaaaaa
     1741 gagcgtcagg tccgctgtaa aatctgcggt tcagttgtgg agccgtttga ctggatgctc
     1801 tctgtggcga aaagagaaac cagactggca gatgatgtaa agctattgcg ccaggaggaa
     1861 caggaaaggc gaaaaaatat agaaaagtta attcagattg agcgtaacgc gaaagcgcgg
     1921 atacgcaggg cgtcaaaatc cagaactgaa taattaaatt cagcactgtt aaaaatttaa
     1981 tccttaaccg gagggattcc tgcaccctca gaacataagg agaccgcctg aaagggtggt
     2041 aatgaaaaat gactgaatta accaaagagc aattaatcaa ggaagccaaa ttaaaaatag
     2101 cggttgcgaa ctgttaccct aattcagaga tggccagggt agagggtgag ctattcaaaa
     2161 ttgcactggc atcgctggaa gcagaaccga tagcgtggga atgcggtgaa aacataatcc
     2221 tgtttaaccc tgacacagtt gaagcatacg caaaacgtgc ggaaatatca cctaaaccac
     2281 tattctccgc gccgccagcg ctggtagtgc ctgataagtt gccgcgtgaa tacagaaacg
     2341 gttggcctct tgcgtatagt gattatgctg aaggctggaa cgactgccgc gaagccatgc
     2401 ttcagggaga taaatcatga ttaatcgtat caagctggag cacatcctcg aatatgccag
     2461 gcagcagagg catattggtc agcattgtaa aattccacca ggagatatgg ttgaaatcat
     2521 ggagattgcc atgcgcaagg ctggcaattc tccggtaact ccggatggtt ggataagctg
     2581 tagtgagcga atgccgccag ttggtgttga tgtacaactg tttgtgtcca gcacgggtga
     2641 acagtttaca ggttttaacc tggataatac gggtgacttc cagtatgcgc agtgtttcgg
     2701 tgcgcccatc gtgtgtgaag ccacacactg gatgccgcta ccagaaccgc cgaaggaggt
     2761 gcgccaatga gctggcctga agccttctca aatgttggta ttgcaatggc ggtggcgctg
     2821 gttgtgtatt cgatttgccg ctggggataa tttgctgaaa aaagatcccg acacacacat
     2881 gagtcgggga tctttgattt atatagccta cgaatccgcc agtaagagag ggggcggacg
     2941 gtttattcta atactggaat tatgtgggga aaagtttata agaaatcggt ttcataactt
     3001 tgtccgccat gataaatacc tgcgataaag actttattgc catcaacggc aaaagcaata
     3061 atcgttctgt ggcggaagtg agttacccgc attccctgtc gaatatcatc gcgtttattg
     3121 ccccgatgcg ggaatgtgga aaacccatca agataatcaa gaagtgcatt ggcataattg
     3181 tcagcaatga cgctccctgc tttctccgtt atatatctgt gcaggttgat tatctgctgt
     3241 tcggcctcag gagtaatgat gacttcatat gtcatgcaga ttacttcccg gctcgaatcg
     3301 cggcgcgaac ctgtgaaatg gagcgtccgt tgtttgggtt ttcgcggata gaatcgagag
     3361 atggggcggc tgaatgcgtt aaccacgctt cgattgcttt atcgcgctca ttcagtgcgc
     3421 gaagcccttc acgaatgacc tcactttctg aagcataggc accggaagcc acacgggcgc
     3481 gcaccatgtc agccatctcg ttagttaatg taatgctgaa ttgttgggtt gtacgcatgg
     3541 taaacctccc ggagtaggat agaacactat tcgatgatag cacactgcct gttgacgaca
     3601 acagaaatca gatacaatat tgccgcacgc cagcctgaac aactggcacc tgctgcgcca
     3661 gcagagacaa ccgatggcgc acgataccag attttacaat tcggataact ccgccgcccc
     3721 tgccagcagg catgggcggc gttctcatgc attcaaatct gactggttcc agcatgaccc
     3781 atgcactgaa gaacaggccg aatggctgat ccagtgctac cgcagacgtg gttacgagtt
     3841 tcagaaagat ctcagtttct atcgtcgtca ctggataatc tctgtcaggc tcccttattc
     3901 cgaatgccca ccgcgtccgt cccgcacatt ccagcagcgc atctggaggt aacgtgcggg
     3961 tattacttcg acctgttctg gtgccggaac tcgggatggt ggtccttaag ccgggccgtg
     4021 aatcaatgtc agcattccat aacggcagaa tattggtgga gccggaaccg aaaaacatgc
     4081 gcggtctgcc gtccggagtc gttcccgccg ttcgccagcc gctggcagag gataaaacat
     4141 tactgccatt tttcagcgat gagcgggtta ttcgtgctgc tggtggtgct ggtgcactgt
     4201 ctgactggtt attacgtcac gtgaaatcct gccagtggcc acacggcgat tatcatcaca
     4261 gcgaaacggt cattcaccgt tacggtaccg gcgcgatggt gttgtgctgg cactgcgaca
     4321 accagctgcg cgaccagaca tcagaatcac tcgatcaact tgctcagcag aatctggttg
     4381 cctggatgat tgatgtcatc cgtcacgcaa taagcggtac gcaggagagg gagttatcgc
     4441 tggccgaatt atcctggtgg gcggcctgca atcaggtggt ggatgcacta cctgaggcag
     4501 tagcgcgtcg ttcgctggga ttaccggcgg aaaaaatccg ctccgtatac cgtgagagtg
     4561 acatcgtacc gggagaacag acagccatca gcatactgaa gcagcgcaca aaaaatattg
     4621 cgctgccact tcacgtccac cagcaacaaa atccaccaca gaaaaaaacg gttgtcagta
     4681 tcgccgttga tccggagtct cctgaatcgt tcatgaggcg gcctaaacgt cgccgttggg
     4741 ttaatgagaa atacacgcgc tgggtaaaga cacagccgtg tgcgtgttgt ggtaagccag
     4801 ctgacgatcc gcatcacctg attggtcatg gtcagggggg agtgggaaca aaggcccacg
     4861 atattttcac gctaccgttg tgccgggagc accacaacga acttcatgca gacccgctga
     4921 agtttgagaa aaagtacggc tctcaggttg agttaatttt tcgttttctt gatcacgcct
     4981 ttgcgactgg cgtgctcggg taaaagaggt gactgatgct catagatttg gttttacctt
     5041 acccgccgac ggtgaacact tactggcgac gccgtggcag cacatatttt gtatcaaaag
     5101 ccggggagcg ttatcgccgg gcagtggcgc ttattgttcg ccagcagcgg ctgaaattaa
     5161 gcctgtccgg acggctggca ataaaaatta ttgcagaacc accggataag cgccgccgtg
     5221 acctggacaa tattctgaaa gcaccgctgg atgcgctgac gcacgcgggg ttgttaatgg
     5281 acgatgagca gtttgatgaa atcaatattg tacgtggaaa gccagtatct ggtggacgtc
     5341 tgggggtgaa gatttacccc ataatgcttg aagggcaggt taaaaaatga aactggaaga
     5401 tttaccgaaa tactactccc caaaatcccc tggcctgacc gatgcatcgg cctcaacgtc
     5461 aaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc
     5521 tgagatgggg ttttctgcgt tccttgggaa aatgggcatt agtatgaatg acagagagcg
     5581 ggcaacagaa ttgctgacag aatatgcact cagtcggtgt gatcgcgtgg cggcgttaag
     5641 aaaactcccg gcagaaataa aaccggcagt gatgcgtatt atggcttcgt atgcgtttga
     5701 agattatgcc cgtagcgcgg cgagcaaaaa acagtgcccc tgctgtcacg gaaaaaaatt
     5761 tattgaaagc gaggttttta caaacaagat ccagtatccg gatggtaagc cgccagtgtg
     5821 ggcaaagtgc acaaaaggcg tgtatccgtc ttactgggag gaatggaaaa aagtcaggga
     5881 ggtggtaaaa gttgcctgtc cggagtgtgg agggaaggga gaggtatcca ctgcctgtaa
     5941 agattgtcgt gggcgcggtg ttgccattca tcgtgaagag tcggaaaaac agggtgtgcc
     6001 ggttttcaga aactgtcagc gttgtggtgg gcgtggctat gaaagattac cttcaacgga
     6061 ggcatttaat gccatatgta atgtaaccga tgccatatct cttgatacat ggaaaaaaac
     6121 agttaaacgt ttttacgata cgctggtggt gcagtttgat attgaagaag catgggcaga
     6181 acaacaactg aaaaaggtga ccagatagct ttgttgattt ttcccgaatc tgtggtaaat
     6241 ttgccctaac gatgggcgtt ttatgcctga cgttagaaga ttttttacac cccgccgcct
     6301 ggcgggtttt ttatgactga aatcgcgtca gtacagtaaa cgcgctggtg gcggtgaata
     6361 ccggtctttc agcttgctgg cttttttgac aagagttatt ggtgtgtcac gttaaccgga
     6421 aaagggaaaa aagcatgcta aaacagcagg atatgacaga aaccgccaga gcagtgttta
     6481 atgaattaag cgtcaccgaa ccggcgacag tcggggagat tgcgcagaat acttaccttt
     6541 cacgcgaacg ctgccagtta atactgaccc agcttgttat ggcgggtctg gcagactatc
     6601 agttcggttg ttacagacgc cttcagtcct gaaggctttt ttatttgtgg taaatgggcg
     6661 gctggtgggt gttaggggca ctcaccagcc atctgctcat gcgtctggat cacaagcaaa
     6721 cctcaggccc actgctttgc gcaaaagcag aatgagccta tcagagacag gcttaatgat
     6781 ccatgtttaa cactgtaaaa atatccagtt gtgagttgat caacgctgac tgcctggaat
     6841 ttatccggtc gttacccgaa aattctgttg acctgatagt cacggacccg ccgtacttta
     6901 aagtgaagcc tgagggctgg gataaccagt ggaagggcga cgatgattac ctgaagtggc
     6961 tggaccagtg tctggcgcag ttctggcggg tgctgaaacc tgccggaagt ctttacctgt
     7021 tctgtggcca tcgcctggca tctgacattg aaatcatgat gcgtgaacgc ttcagtgtgc
     7081 tgaaccatat tatctgggcg aagccgtccg gacgctggaa cgggtgcaac aaggaaagcc
     7141 tgagggcgta tttccccgcc acagagcgca ttctgttcgc ggaacattat caggggccgt
     7201 atcgtccgaa agatgccggg tatgaggcga agggcagggc actgaaacag catgtgatgg
     7261 ctccgctgat ttcttacttt cgtgatgcgc gcgctgtcct ggggataacg gcaaaacaga
     7321 ttgcagatgc cacaggaaag aaaaacatgg tgtcgcactg gttcagtgcc ggtcagtggc
     7381 tgctgccgaa cgaaagcgat tatctgaaat tacaggcgct gtttgcccgg gtggcagaag
     7441 agaagcatca gcgcggtgaa ctggaaaagc cccaccacca gctgctggag acgtatactt
     7501 cactgaaccg gcagtatgcg gaactgcaga gtgaatataa acatctgcgg cggtattttg
     7561 gcgtgacggc gcaggtgccg tacacggatg tgtggacgca taaaccggtg cagtactatc
     7621 ccgggaaaca tccgtgcgaa aaaccggcag aaatgctgca gcagataatc agtgcgagca
     7681 gtcgtccggg tgacctggtt gcagatttct tcatggggtc gggttcgaca gtcaaagccg
     7741 cgatggcgct ggggcgtcgt gcaactggcg ttgagctgga gactgaacgt tttgagcaga
     7801 cggtcaggga agtacaggat ttagtcagtc agaacggatg atattgcagg attagttacg
     7861 taccgttatt atcctgcgcc cggcccttta gctcagtggt gagagcgagc gacgcataat
     7921 cgccaggtcg ctggttcgaa tccagtacaa cgcgccacac ttattttccc tggctcgctt
     7981 ttgcgggcct ttttttaaat gtctcacaat tcaggcggtt gactgttgtc tggtttgcgg
     8041 ggagtttgtt aaaagaaact ggcatggtga atccccctgt gcggaggggc aatcagcaac
     8101 caggtatatg gggtaatcgc ggattcaggt gctggtactg aattcaccgg gaggcacccg
     8161 gcaccatgca agaaaaagaa tgtgcatgca aacatgcccc tctccggagg ggctttttta
     8221 tgggt
//
LOCUS       DAZGTR010000167         8132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGTR010000167 DAZGTR010000000
VERSION     DAZGTR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.169"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..141)
                     /locus_tag="ACHLCY_004399"
     CDS             complement(<1..141)
                     /locus_tag="ACHLCY_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371511.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFF"
     gene            219..1313
                     /gene="ddlA"
                     /locus_tag="ACHLCY_004400"
     CDS             219..1313
                     /gene="ddlA"
                     /locus_tag="ACHLCY_004400"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0371512.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1337..1549)
                     /gene="yaiZ"
                     /locus_tag="ACHLCY_004401"
     CDS             complement(1337..1549)
                     /gene="yaiZ"
                     /locus_tag="ACHLCY_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP0371513.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1809..2117
                     /gene="yaiY"
                     /locus_tag="ACHLCY_004402"
     CDS             1809..2117
                     /gene="yaiY"
                     /locus_tag="ACHLCY_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP0371514.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2176..3270)
                     /gene="yaiW"
                     /locus_tag="ACHLCY_004403"
     CDS             complement(2176..3270)
                     /gene="yaiW"
                     /locus_tag="ACHLCY_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP0371515.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3283..4503)
                     /gene="sbmA"
                     /locus_tag="ACHLCY_004404"
     CDS             complement(3283..4503)
                     /gene="sbmA"
                     /locus_tag="ACHLCY_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP0371516.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFGAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4855..6012
                     /gene="ampH"
                     /locus_tag="ACHLCY_004405"
     CDS             4855..6012
                     /gene="ampH"
                     /locus_tag="ACHLCY_004405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP0371517.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMVPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6013..6636)
                     /gene="iprA"
                     /locus_tag="ACHLCY_004406"
     CDS             complement(6013..6636)
                     /gene="iprA"
                     /locus_tag="ACHLCY_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP0371518.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6724..>8132)
                     /locus_tag="ACHLCY_004407"
     CDS             complement(6724..>8132)
                     /locus_tag="ACHLCY_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0371519.1"
                     /translation="LEDSGFYGNSAEPSDYSGNGEANDVALYFSDSAASNYSMKNNVY
                     FSNSTLLGDVVFASTFNANFYPQGHDSNADGVLDTNGGWADDSLNVDELNITLDNGSK
                     WVGSATTSANVDDESTASTDWYDVTGNSLYPGVVTEANAWGRTIDDQVFQSGVFNVTL
                     NNGSEWNTVNASNIDTLAVNNGSQVNVSESSLISDSIALTNGSSLNIGDEGYVGTDHL
                     TVDSYSTVALSSDDNSSIYNGYYTPSLLANTITVTNGGLLDINVDQFDDNPFQTDTLE
                     LTSGNIADHNGNVVSGVFDIHSSDYVLNADLVNDRTWDTSKSNSDAWGFGLKAGYDFK
                     LGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQA
                     LTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLG
                     GGDVDQDWSANVGVKYTW"
BASE COUNT         2118 a         2060 c         1915 g         2039 t
ORIGIN      
        1 aaaaaactca ccgcatggcc atttaagcca ggaataaatt agcttcccgt tcatcatttt
       61 tgtgatcttg cgcacatctt tgagataacg cactttcttc accagtacgt tgcacgcttt
      121 gtcagcagaa gattgcaaca atctttcaga aggccgcgaa tcccccgttt tgtcgtggta
      181 atgtatgcac tttgtgttag acgggatagg tttttaagat ggaaaaactg cgggtaggaa
      241 tcgtttttgg tggtaaatca gcggaacatg aagtgtctct gcaatcggca aaaaacattg
      301 tcgatgccat tgataaaagt cgcttcgacg ttgtgctgct gggcattgat aaacaagggc
      361 aatggcacgt cagcgatgcc agcaattatc tgctaaatgc agacgatcct gcccatattg
      421 cgttacgccc ttcggcgacc agccttgcgc aggtgccagg taaacatgag catcagctta
      481 tcgacgcgca aaacggtcag ccgttgccga cggtggatgt cattttcccg attgtccacg
      541 gtacgctggg cgaagatggt tccttgcagg gaatgctgcg ggtcgccaat ttaccgtttg
      601 taggttctga tgttctggct tcagcagcct gtatggataa agatgttacc aaacgtctgc
      661 tgcgcgatgc cgggctgaac attgcgccat ttattaccct gacgcgcgcc aatcgtcaca
      721 acattagttt tgccgaagtg gagtctaaac tggggttacc actgtttgta aaaccggcta
      781 atcagggctc ttctgttggt gtcagcaaag taaccagtga agaacagtac gcaattgccg
      841 tcgatctggc gttcgaattc gatcataaag tgatcgttga gcaagggatc aaaggtcgtg
      901 agatcgaatg cgcagttctg ggcaacgaca acccgcaagc cagcacctgt ggcgagatcg
      961 tactcaccag cgacttctat gcctacgaca ccaagtacat tgacgaagat ggcgcgaaag
     1021 tggtagttcc ggcagccatt gcgccagaaa tcaacgataa gatccgggcg attgccgttc
     1081 aggcttatca aacgttggga tgcgcaggca tggcgcgtgt agacgtgttt ttaaccccag
     1141 aaaacgaagt ggtgatcaac gagatcaaca cactgcctgg cttcaccaac atcagtatgt
     1201 atccgaagct gtggcaagcc agcggtctgg gttacaccga tctgatcaca cgtttgattg
     1261 agctggcgct ggagcgtcac gctgcggata acgcattgaa aaccacaatg taatatttta
     1321 acgcccgata atattcttat tgggcgtttt cagtttcttc atcgcgacga cgaataatca
     1381 gccccgccag ccagaaacta atcacccacg tcaccagacc gacggcatag gtctgccaac
     1441 cttttgcttc aaatcccagt aaccccacca cgccattaag aatgaatata aggccaatgg
     1501 cgaacgcata gtagtgccag tcacggcgga tttttacagg cagattcatg gcagctccgt
     1561 taacgaaaaa caacatcctt gcatattctg taacaaccgt ctgtggtgta gacaacattt
     1621 tcgtaattgt taataaattt aacttaaggt tgccattttg tgactcacaa cgaagattca
     1681 cgcttcggga atattcccaa catcaattta ccatgattct gatattgaca atctaaatga
     1741 cctgtcagac ttgccataag ccgctggtat cacaccagaa gagaagcaga tgacttccgg
     1801 aggtttttat ggctgatttc accctgtcaa aatcgctgtt tagcggaaaa tatcgcaatg
     1861 cctcttcaac gcctggcaac attgcctatg cgttgtttgt gctgttttgc ttttgggctg
     1921 gggcgcaatt gctgaacctg ttagtgcatg cgcccggcgt ctatgagcgt ttaatgcagg
     1981 tccaggaaac aggtcgccca cgggtggaaa ttggtttagg tgtcggcacc attttcgggc
     2041 tgatcccgtt tttagtaggc tgcctcattt ttgcagtggt ggcgctatgg ctgcactggc
     2101 gacatcgccg ccagtaagat ttacgttgcc agatacggcc taaaacgcct tatctggcat
     2161 gacattcctg tttgatcact gtttcataca acgcgcccgc cgttcgtcta cgcgcttcgc
     2221 aaaccaggcc gtagtcaggt tgcgggtgat tttcgggctt tccagttgaa tgccaggcaa
     2281 catttcgcgg ggtagtgatt tacccgtttt cgcttcggca agttgataaa ctttcttgta
     2341 cagcgccgtc tcctcaaaag agaagctgtc acctttctct aactgacggc gaatttcgct
     2401 gtcgctcatc cccagttttc ccgccagttt gcgcgttgcc agttcggttt tcccgggttc
     2461 tttactgtcg taacgaatta aatcaccgtc cagtgccagc ttcacgccgc tggccttact
     2521 gactgcgttc tgaaacgcgg cattacggct ggcgtaccag ccagcgttaa aatcagcaaa
     2581 gcggtatatc ggtgcgctat agctggcggg ataattcagt aaatggtaag taccaaacca
     2641 caacccgccg cggcgactga agacttcctg acgaactgta ccgtccattt tccacggata
     2701 ccctttggta tgctgttcgg caaaagcaat gctgacctgc atcggcccac cggtgcgcac
     2761 aggattaagc gaaccaaaca gcgtctgccc cattggtacc atgctgatca gatcgtcaaa
     2821 aatcgcgctc aattgctttt ctgtacgcac cgaatctaac cgttcgctat aacttttgcc
     2881 gtttggcgat ttgattttta gcgcggtatg aaccagaaaa gcaggaatgt gcatccgttc
     2941 agcacgacgg tcaatctctt gccaggcgat cttgcttaag cccggaacgg ccggatctgc
     3001 ctgataattt gactcctgct gcgccaccgc cagcaccgag cagacgtttt ccagcgtcgg
     3061 tgccagcccc tgactttcaa aggtggtggc gagatctttc gcccaggcat cgcggtcttt
     3121 cacgctcgca ggcatcttct gccgcacgac gctcgctaca tctatcgctt tttcgccctt
     3181 tttcaacggt tgcggagcct gactactaca ccccgctaaa accagaaccg ccaacaatga
     3241 cagcgaagat agatgattga cgcgcgacat tacttctcct ttttagctca aggtatgggt
     3301 tacttcctga attttgtcac catccagctc atgttcaaag ctgcgcagac gtttgtagat
     3361 agacatcaac tcaaccagtg tggtccatga gttaatcagg tactggaaag caccgcgaac
     3421 ctgaccaaaa acgttggtaa tctgcgtcat caggccgagc gtaatcgtac cggcaacaat
     3481 tgacggaaac agcaagaaca aaccgaaaac gttatcgacc tgcaaataga gaatgcgggc
     3541 gatgttgaaa tacatatagt gaaaatagag gcgaaaatag tttttccgta cggcgccaaa
     3601 cagctcgcgt accgtaggcg gcgtcgcgcg cgtggcatcg tcttcaccat aaaccagctc
     3661 tttacggtag gcggcctcta ctcgctggtt tttaaactcc agccccggca gtttgatccc
     3721 taccactgcc agcaatccgg tccccatcag cgaccagacg atagcggcaa tcaccagacc
     3781 atacggaatg tgcccgataa tcggcagctc cggcacatgc gcggagagcg ttaccagcac
     3841 cggcagaaag gcgatcaacg tcatgatggc gttgataaaa ctgacgccca tattctccag
     3901 cgttgaagca aaacgcatgg tgtcttcctg cacacgctgt gcggcacctt cgatatgacg
     3961 cagttgttgc cagttcgcca tgtaatattc gttcatcgcc gtacgccagc ggaacacata
     4021 gtgactgaca aagaagttgt tcagtacact gatcaccacc gcgatcagcg caatccccag
     4081 aaagacgccc acttcgcggt aaaattgttc gatagtgact ttatgcggcg aacttagcgc
     4141 ggtttgaatc agatcataga acggtgcata ccaggcgttg acggcgaccc cgacttccac
     4201 caaaaaccag gtgacgaaga tgatcagtgc agtaccgagt attgaccagt attgccaacg
     4261 gtgcgggctg tagataaacc agaaaaatgc aaaaagtcct acgcaaacaa tgtagtaagc
     4321 gtaaaaaatc aggaaatcca acgaccagaa acgcgcggcg ctaatcggga tctgcccgga
     4381 agcgccggtg atacgcgcca cccagtcacc cccaccggct tgccagaaga taacggcaat
     4441 caatgcccaa acaaaggccg agagaaaaaa cgttcccggc tttgggaaaa aagacttaaa
     4501 catatgcact cctgctaact tcttatcgtt ttaacaactg ctgtgtatta accgcatgac
     4561 cgctacaaag cgtcagccaa atgaaaccgc ccattgtgac ccatagcaag gcgcttagtt
     4621 cgccagcctg accataagaa ttgtttcgac ggatttcaca aaccaaagat tgtttacata
     4681 agcggattaa cacgcgcagg atcacaaatg caggcattgg tgagcaggaa aaagatcgtg
     4741 tttacgcgcc gttacgtttt taacactatt attgcactaa ttcgcccttt gcaatccatc
     4801 aatgagtagt ataaatacgc tcagttacct tcattcaatc tatggacacc accgttgaaa
     4861 cgtagtctgc ttttttctgc cgtgctgtgt gcggcgtcat tgacctccgt ccatgcggcg
     4921 cagccgatca ctgaaccgga gtttgcctct gatattgtcg atcgttatgc cgatcatatt
     4981 ttttacggca gcggtgccac ggggatggcg ctggttgtta tcgacggtaa ccagcgcgtc
     5041 tttcgcagtt atggcgaaac gcgacctggt aataacgttc gcccgcagct ggattcggtc
     5101 gtgcgtattg cttccctcac caagctaatg accagtgaaa tgctggtgaa attgctcgac
     5161 caggggaccg tgaagttaaa cgatccgcta agtaaatatg ctccgccagg cgcacgcgtg
     5221 ccaacctaca atgggacgcc cattacgctg gtcaatctgg caacccatac cagcgcgctg
     5281 ccccgtgaac agcccggtgg cgcggcacat cgtccggtgt ttgtctggcc aacgcgcgag
     5341 caacgctgga aatacctttc tacggcgaag ctgaaagccg cgccaggcag tcaggcggcg
     5401 tactctaacc ttgcgttcga cctgctggct gatgcactgg cgaatgcgtc cggcaagcct
     5461 tatacccagt tgtttgaaga gcagattacc cgtccattgg ggatgaaaga caccacctac
     5521 accccctcac cggatcagtg ccgccgttta atggttgcag agcgcggtgc cagtccatgc
     5581 aataacacgc tggcggcaat tggcagcggc ggcgtctatt ccacgcctgg cgatatgatg
     5641 cgctggatgc agcagtattt gtcatctgat ttttatcagc gcagtaacca ggcagatcgc
     5701 atgcagacgt tgatttatca gcgcgcgcag tttacgaaag tgattggcat ggatgtcccc
     5761 ggcaaagcgg atgcgcttgg tttaggctgg gtatatatgg tcccgaaaga gggtcgtccg
     5821 gggattattc agaagacagg aggtggtggc ggtttcatta cctatatggc gatgatcccg
     5881 cagaagaata ttggcgcgtt tgtggttgtc actcgttcgc cgttgacgcg ctttaaaaat
     5941 atgagcgatg gcatcaatga cctggtgacc gagctaagcg ggaataaacc gctggttatc
     6001 cccgcgtcct gattaatact ctgaaggcaa acggttgata gcgaccagtt tgcctttatt
     6061 catttcgata tagccgcctt tacgcaaagc agcgagaact tctgcgacga cagaacgcga
     6121 tatgcgtgta cgttgatgga tataattcat cacaccaata cgtgatcgca attcttcatt
     6181 ccagtcaatc attgataata atgttgcgcg gatttgttcg taggaattat gcccaatgag
     6241 ctgcacgtcg cgtaattcca gaattctatt ttgccaggct aaccagtaaa aagcgtctcg
     6301 ccagagttga ttttgttcaa taagcgtaat ggtttgtttg gctggtagat gatatcccgt
     6361 acaatttcct tctgatatta atttgtatgg tatatcgttt ttcattaaac catcagccag
     6421 ccccataata taaggagcct gggtaatacc gataagtacg ttttcttctc tacgcagaga
     6481 gataactccc tctaaaataa cgaaagtatc ttcgttattg acatcactgg aaaatataac
     6541 ttgcttttca ttattaaact cgaagcgcgt accgtatctg gacaaacatt tatcgagctt
     6601 accaaattcc tgaagaggtt taactacaga taacatttgc gcgtcctttg cagtaatgcc
     6661 cgtcaaatcc ttgacgggca ttatttagat taaattacca gaatttcttc ggagtgaaga
     6721 atattaccag gtatatttaa cacccacgtt cgcggaccag tcttgatcta cgtcaccacc
     6781 accgaggtag ttagcatcgg tataggcgct gaagttcttg gtgaagctga actgagtacc
     6841 cagaccaaca cgtaccgcag acccttcagt accgttatcg atggaatcac cgttcacatc
     6901 gttatcgttg ttagagtcgt cgtagacgta agccagtttg aagtacggag tcagagcctg
     6961 gtcttcgctg taggtgaagg tataacctgc atctacaccc agttcataac gcatgctgtc
     7021 gtaagactga ccgtcaactt tcatgtcgtt gctcagctgg tagtcatcac cagactggaa
     7081 cagaccagaa acgctgccgt aaggagtcac gtaaccagca tcacccagtt tgaagtcgta
     7141 accggctttc aaaccgaagc cccaagcgtc ggagttggac ttggaagtat cccaggtacg
     7201 gtcgttcacc aaatcagcgt tcagaacata atcgctgctg tggatatcga atacaccaga
     7261 cactacgttg ccgttatggt cagcgatgtt gccgctggtc agttccaaag tatcagtctg
     7321 gaacggatta tcatcgaact ggtctacatt aatatccagt aaaccaccgt tagtcacagt
     7381 tattgtattt gctaacagcg acggagtgta ataaccatta tagatacttg aattgtcatc
     7441 actagacagc gctacagtac tgtagctatc aactgtcaga tgatcagttc caacataacc
     7501 ttcatctcca atgttcaaag aagaaccatt ggtcagagcg atgctatcag aaatcaagct
     7561 agattcagac acattaacct gagagccatt attcacagct aaagtatcaa tgtttgaagc
     7621 gttaacggta ttccattcag aaccattatt cagagtgacg ttaaacacac cgctctggaa
     7681 tacttgatca tcaatcgtac gaccccaagc atttgcttct gttacaacgc ccggatacag
     7741 gctattacct gtaacatcat accagtcagt cgaagcagta gattcatcat caacatttgc
     7801 tgatgtagta gctgagccta cccacttaga accattatcc agagtgatat tcagttcatc
     7861 aacattaagt gagtcatcag cccagccacc attggtgtcc agtacgccat cggcattact
     7921 gtcatgacct tgaggataga agtttgcgtt gaaagtactt gcgaaaacaa catcacccag
     7981 cagagtagaa ttgctgaaat aaacattgtt tttcatggag taatttgaag ccgcactatc
     8041 actgaaatac agagctacat cattagcttc gccgttacca gagtaatcac taggttccgc
     8101 agagttacca tagaaaccac tgtcttccag ag
//
LOCUS       DAZGTR010000168         8001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGTR010000168 DAZGTR010000000
VERSION     DAZGTR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.170"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..836
                     /locus_tag="ACHLCY_004408"
     CDS             <1..836
                     /locus_tag="ACHLCY_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0371520.1"
                     /translation="ENGEIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEY
                     KARGHNRMGQIFRKVLIDVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMM
                     IYNAAEKDGYFLQRGMLLALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLA
                     LRCDKLNLTLDDVVSSCRDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSV
                     DYNWRANGTPPSFHEQRSLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLV
                     I"
     gene            complement(882..1520)
                     /gene="ompW"
                     /locus_tag="ACHLCY_004409"
     CDS             complement(882..1520)
                     /gene="ompW"
                     /locus_tag="ACHLCY_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP0371521.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1760..1849
                     /locus_tag="ACHLCY_004410"
                     /pseudo
     CDS             1760..1849
                     /locus_tag="ACHLCY_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1877..2620
                     /gene="yciC"
                     /locus_tag="ACHLCY_004411"
     CDS             1877..2620
                     /gene="yciC"
                     /locus_tag="ACHLCY_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP0371522.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSVGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2650..3189
                     /gene="yciB"
                     /locus_tag="ACHLCY_004412"
     CDS             2650..3189
                     /gene="yciB"
                     /locus_tag="ACHLCY_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP0371523.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3294..3692
                     /gene="yciA"
                     /locus_tag="ACHLCY_004413"
     CDS             3294..3692
                     /gene="yciA"
                     /locus_tag="ACHLCY_004413"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP0371524.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3732..4451)
                     /gene="tonB"
                     /locus_tag="ACHLCY_004414"
     CDS             complement(3732..4451)
                     /gene="tonB"
                     /locus_tag="ACHLCY_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP0371525.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4542..4832
                     /locus_tag="ACHLCY_004415"
                     /pseudo
     CDS             4542..4832
                     /locus_tag="ACHLCY_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(4838..5073)
                     /locus_tag="ACHLCY_004416"
                     /pseudo
     CDS             complement(4838..5073)
                     /locus_tag="ACHLCY_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5244..5894)
                     /locus_tag="ACHLCY_004417"
     CDS             complement(5244..5894)
                     /locus_tag="ACHLCY_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0371526.1"
                     /translation="MPFSFNLSSGNYLSTQDVEVLQRATRDHQMERLTIGERSFSVRY
                     QSAMDAFIVDPVQGELYSGLSHTELADIIRLADSVENQLNGGNSFLDVFSTYMGQVIS
                     EFMHSNDNRIELLQRRLHSCSFLVNIEEMSYIDEALQCPITLAIPQRGVFLRNAEGSR
                     VCSLYDEMALSRIINDGMHHPLSREPITLSMLVAREQCEFDCSIGHFTVRSDCYSV"
     gene            6126..7182
                     /locus_tag="ACHLCY_004418"
                     /pseudo
     CDS             6126..7182
                     /locus_tag="ACHLCY_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(7401..7991)
                     /gene="espM1"
                     /locus_tag="ACHLCY_004419"
     CDS             complement(7401..7991)
                     /gene="espM1"
                     /locus_tag="ACHLCY_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector guanine nucleotide exchange factor
                     EspM1"
                     /protein_id="HFP0371527.1"
                     /translation="MPVNATGVSFSSFGISYHKDNSFRGTIRGKNDEVVKCSMGERSI
                     RFNVNKFSGCILETVSRQSTKDIHGWVSDERTVYPSRVINQEIDNCCLQKNAKISSEE
                     RKMVFSLVSKEFELTLDVKAAQSSINHIIIGNASFGKKMDALCDGMSRAVKNTTTDYI
                     ANVLADKFYQKHIAPGVDIVKLRNEIPGYMSRVIQG"
BASE COUNT         2134 a         1885 c         1786 g         2196 t
ORIGIN      
        1 atgaaaacgg cgagatcaaa ctcaacacgc tgaaacagaa agcggcccct ctcagggtat
       61 ttgatgaacg ttttggcacc agaccgttag atgccataac cgtaaaagat gtggtatcag
      121 tactggaaga gtacaaggcc agaggacata acagaatggg acaaattttc aggaaagtac
      181 tgatcgatgt tttccgggaa gctcagcaaa cgggcgatgt cccgccaggc tttaaccctg
      241 cagaatctgc aaaaaaaccg caggtgcgga tatcaagaca gcgactgact tttgatgagt
      301 ggatgatgat ttataacgca gcggaaaagg atggttactt tttacagcgc ggtatgctgc
      361 tggcactgat gacaggccag cgcctttcag atatttgcaa aatgcaattt tcggatatcc
      421 gggatggtta tcttcatgtc gaacagcaaa aaacaggaac ccggattgcc atccctctgg
      481 ctctgcgttg cgataaatta aatctcaccc tggatgatgt ggtgtcatcc tgccgcgatt
      541 gcgttcttag tccgtggcta ttgcaccacc atcacgcgaa agggacagct aagcgcggcg
      601 ggatggttaa gccagcaaca ttaaccgttg catttaaaaa agcccgggat tctgtggatt
      661 acaactggcg tgctaatggc acccctccct ctttccatga gcagagatct ttatcagagc
      721 gattgttcag agagcagggg gttgatacca aaattttgct aggccattcg aatcaaaaaa
      781 tgaccgatat ttacaacgac gcacgcggta aggaatggaa aaaactggtc atttgatgac
      841 cagttttgca gaggggtttt gcagaggttt tgcagagaaa attaaaaacg atatcctgcg
      901 gagaacataa acacccacgg atccagtcgt accgagtctt ttacggtagt aacaccagat
      961 ttgtacttag ccgtggtatc gatatccatg taccacactg acatgttaac caaccagtca
     1021 cggttaatca gataatcaac ccccacctgc ccggcagctc cccaggaatc tttcagactg
     1081 agatcggaaa gccccgcctc tttgccatga tcgttaaatc cattatcaaa gaaggtggtg
     1141 tagttaatac cggccccaac gtaaggacgg aatttgctgc tggcatcacc aaaataccac
     1201 tgcgccatca gtgttggcgg cagatggtga acggttgcaa tatcgccggt cgcccgggtg
     1261 ccgattttat ggcggaacgg cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc
     1321 atataagtaa acgtcaagcc cagttgagtg ttattggtca cgctgaatcc acccagactt
     1381 cctaacgtac caccagcacc ttctgttgga cgtacggttg cagaacctgc acgcataaaa
     1441 aattcgcctg cttcatgcgc aaaggcactg ccagaaagaa gagttgttac tgccaaagcc
     1501 gccactgtta actttttcat atccgctccg tcgttatggt tataaaaacg tgacgaatat
     1561 acctacattg gagtaacaaa tgatcctaca tagatcacat tacatatgat aattcaccat
     1621 ttattgatct ggattaattt agaagttgca ttgagaatcc tggttaatgt gatttaaatc
     1681 aatttttaaa tattttctct acgcaaatat taactcgatc gccacttgcc gcggtttgcc
     1741 cacgcctgaa ggccagcaat tacctccgct ttacaaagat atgcttttcc atacaagacc
     1801 tgatgctgcc acccgaggct taaccagggt acaattgccc gctaattaac acctgcataa
     1861 aactcaagga gagtgcatgt ctatcacggc gcagtccgta taccgtgaca ccggaaattt
     1921 cttccgtaat caatttatga ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt
     1981 ggtgttaggg catgttttct cacccagtga tgcacagctt gcgcagctca atgacggcgt
     2041 gcccgttagc ggcagtagtg ggttgttcga cctggttcag aatatgtcac cggaacagca
     2101 acaaattttg ctgcaggctt cagcggcgtc cactttttca ggattaatcg gtaacgccat
     2161 tctcgccgga ggcgtaatat taattatcca gctggtgtct gtgggtcaga gagtcagtgc
     2221 gctgcgagct attggtgcca gtgcgccgat attgcccaag ttatttattc tgatttttct
     2281 gactaccctt ttagtacaga ttggcatcat gctggtggtc gttccgggaa ttatcatggc
     2341 cattttactg gccctggcac cggtgatgct ggtacaggac aaaatgggca tttttgcctc
     2401 gatgcgtagc agtatgcggc tgacttgggc gaatatgcgt ctggtggcac ccgcagtact
     2461 gagctggttg ctggcaaaaa cactgttgct gctttttgcc tcttcttttg ccgcattaac
     2521 cccggaaatt ggtgccgtac tggcgaacac cttgagcaac ctgatttctg ccatattgct
     2581 catctatctg ttccgcctgt atatgttgat tcgccaataa ccttaagcat ttgattacgg
     2641 aatcgtaaaa tgaagcagtt tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac
     2701 aagatttatg acatctatgc ggctactgcg gcgctgatcg tcgccacggc gattgtgctt
     2761 atctatagct gggttcgctt tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg
     2821 gtggtcgtct tcggtggctt gacgctgttc ttccacaatg atgagtttat taaatggaag
     2881 gttacagtca tttatgctct gtttgcggga gccctgttag tcagccaatg ggtgatgaaa
     2941 aagccgctaa ttcagcggat gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg
     3001 aagctaaatc tggcctgggc tgttttcttt atcctttgcg gtctggcaaa catctacatc
     3061 gcattctggc tgccgcaaaa tatttgggtc aactttaaag tctttggcct gaccgccctt
     3121 accttaatct tcacattgtt aagcggtatc tatatctacc gccacatgcc gcaggaagat
     3181 aaatcctaac cagactgcca gtccttcggg gctggcgctg tatctcctct gttctcctca
     3241 ggaaaatcat agtagcatcg cgcctgtgat tttcctttta agtcggtttt accatgtcta
     3301 caacacataa cgtccctcag ggcgatcttg ttttacgtac tttagccatg cccgccgata
     3361 ccaatgccaa tggtgacatc tttggtggtt ggttaatgtc acaaatggat attggcggcg
     3421 ctattctggc gaaagaaatt gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga
     3481 ctttcttacg gccggttgcg gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga
     3541 aagggacgac atcggtcagc attaatattg aagtgtgggt gaaaaaagta gcgtctgaac
     3601 caatcgggca acgctataaa gcgacagaag cattatttaa gtatgtcgcg gttgatcctg
     3661 aaggaaaacc tcgcgcctta cctgttgagt aatagtcaaa agcctccggt cggaggcttt
     3721 tgactttctg cttactgaat ttcggtggtg ccgttaattt taaacaggat attcaccaca
     3781 atcccactgc ctggcttacc cggctcataa cgccatctgc gcatcgcatt tttcacctca
     3841 cgctcaaaca tgttcgcagg cttggctgag aggatttgta cgttatccac gcgaccatcc
     3901 ggcgtgacgt caaatttaac tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc
     3961 ggatactgcg gctgattacg gcttaatgcg cgtggtcctg aagccacact ggtaaccggc
     4021 ttgctggttg cagccgttgc cgtacttgat gtggggcgcg cgggtgccgt attttcaaac
     4081 ggtgatgccg gacgcgactc tacgggtttg atatcgcgtt ttggctgctc ctgtaccttt
     4141 ttcaccggct tcggttttgg cttaggtttc ggcttcggct tttcaatgac caccggtgct
     4201 tcttttggcg gttcggggat cggctcaggt tccggttctg gctctaccac cggctccggt
     4261 ggcggctgaa cggcttgtgg cggttcgaga tcagcaggcg caaccatcgt gacagaaatt
     4321 ggctgcgcag gcgcaggtag ttcaataacc tgatgtaccg aggtatagag cagacccgcc
     4381 acaacagcac catgaatgca gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa
     4441 tcaagggtca ttgtattgta gctattcggc attgtagcat tagccataac gtttataagt
     4501 tcatctttaa atgataatca attgcatggg agtttatcac cgtgagaacc gtaataacat
     4561 accttcggtt cagttcagct atacaaggcg ctgaaggtgc agattcaacc agacgacaaa
     4621 atgacctgtt caagcaatgg ttgaagaaga atagcgatgc tcaagtagta gcgtctttca
     4681 gtgatgaggg tctatccggt tacaaaggta agcatcttac tggtcagttt ggtgacatgt
     4741 tagcccgtat tgagtctggg gagtttccag aaggtacaat tctgctagtc gaagctatcg
     4801 accgcatagg ccgccttgaa catcttgaaa ctgtttttca aaacagttcc gtgctgagac
     4861 agccacaagt actgtgtcgg caccaccgat tgtaatcaca ctagatctaa ttaaacacag
     4921 ccgtctgcat tatttcccga ctcataaaat attagccgta aatgcattcg acttttagtg
     4981 agtggaagaa actttccgcc ctagcattat cgtaacagcg cccctttgca ctcatcctgt
     5041 catgcaggta tgccgcttca gaagcgcctg ataatgcgtt gaaatcaggg cggtggctac
     5101 ttgctattca gttcacgata caaaatgggc aatatccctt aaggaccagc gcatagcaat
     5161 gagtcacttt attatcaagt ttgatgaccg ctttgatgct aacttttaat aagagtctct
     5221 tcacacaaaa tcgtgtaccg agtctacact gaataacaat cactcctcac cgtaaagtga
     5281 ccgatactgc aatcaaactc acactgctct ctggccacaa gcattgataa tgttattggc
     5341 tctctgctta gtgggtgatg catcccatca tttattatac gagaaagagc catttcatca
     5401 tataaactac atactctgga accttcagca tttcttaaaa aaacacctcg ttgaggaatt
     5461 gccagagtaa tcgggcactg taatgcttca tctatgtaag acatttcttc aatattaact
     5521 aaaaatgaac atgaatgtaa tcgccgctgt aacaattcaa ttctgttatc attactatgc
     5581 ataaattcag aaataacctg ccccatatat gtactgaata catcaagaaa tgaattgcct
     5641 ccattcaatt gattttcaac agaatcagcc aatctaatga tatcggctag ttctgtatgg
     5701 cttaaacccg aatatagttc cccttgaaca ggatctacaa taaaagcgtc catagcactc
     5761 tgatagcgaa cagaaaacga tctttctcct attgtgagtc tttccatttg atgatctctg
     5821 gtcgcacgtt gtaaaacttc aacatcttgt gttgacaagt aatttccaga agacaaatta
     5881 aatgaaaatg gcataatttt atccctaaag catatatctt acttttcgat aattaacaaa
     5941 aagccatcct ccacagcaat actccatcag aataattgta ggattatttt aatctaattt
     6001 ataagcataa aatatatact cactaaatag ctgcgcagaa tagtagatca tttagaggga
     6061 actcaacccg attgtgcgat ctgatcaatc gccaaatcaa tacaaatcac caaccggact
     6121 gagcaatgtc gatcatacca ccaatttccc gtgacgaacg acgcctaata cagaaagcca
     6181 tccataaaag gctgacagcc atgctgatgc tgcaccgggg cgaccgtgtc agcgacgttg
     6241 cccgcacact atgctgcgcc cgttcctctg ttgggcgctg gattaactag ttcacacagt
     6301 cgggtgttga gggactgaaa tcattacctg ccgagcgagc ccgccgctgg ccgtttgcgc
     6361 atatctacac actattacgt gaacaggtaa aatattcact cgcgactttg gctacaatta
     6421 ttcacgctgg agtacagaac tgctgacaat aaaaatcaat gagataaccg gctgcctgtt
     6481 acatgctgga accattcgcc gctggttgtc gtctgtgggg cttgtgtggc gaagggctgc
     6541 gccaactcta cgtatccgtg ccccgcctaa agataaaaag atggcaacaa tccataacgc
     6601 actggacgaa tgcagcacag agcatccggt cttttatgaa gatgaagtat ttatccatct
     6661 taatcccaaa atcggtgcgg actggaaact gctcgggaag caaaaacgag gggtcacgcc
     6721 ggaacaaaat gaaaaatatt ctctggacgt agcgctgcac agcgggacag gttaagtcag
     6781 ctgtgtgggc gccaacagca aaagttcggc gctgttcatc agactgctga agcggcttaa
     6841 agctacatac tgtcggacga aaaccatcac gctgctcgag gacaactaca ttatccacaa
     6901 aggccgggaa acccagcgct ggctgaagga taaaccgaag tgcagagtca tttatcagcc
     6961 ggtttaatct ccatgggtga atcattttga atagctatgg cagatacttc acgccacaat
     7021 aacgcgtaat catcagtgcc gctcaatgtg gcaactattg aaaaagttcg ccattttatg
     7081 gaatccgtca gtccattccc cgaaggcaaa catgggctgg caaaaatgtg cgatattagg
     7141 cgcagctatt tagttattcc gtcatttctc ccgcccgtgt aaacactact gaatcttgtc
     7201 gttgtgacaa agctataaat agtcaatcac agcacgtttc tgcgtacgac gcgctgcaat
     7261 tttctttacg atttataact gtacaggtga actatgagtt atttggatac atacatatag
     7321 tgtaacgcct gttgtatatt ttactcatat ctttttacag gatgaaaacc aatgatactg
     7381 gcgcatagcc tcctccaacc ttacccctgt ataacacgac tcatataacc tgggatttca
     7441 tttcgtagtt ttacaatatc aacacccggg gcgatatgtt tctgataaaa cttgtctgca
     7501 agcacgtttg ctatgtaatc tgttgtagtg ttttttacag ctcggctcat accatcgcaa
     7561 agagcatcca tttttttgcc aaaagaagca tttcctatta ttatatgatt aatagaactt
     7621 tgtgccgctt taacatcaag agttagttca aactccttac ttacaagaga aaagaccatt
     7681 ttcctctctt cagaagaaat ttttgcattt ttttgaaggc agcaattatc aatctcttgg
     7741 ttgataaccc ttgacggata tactgtccgt tcatcactta cccatccatg aatatcttta
     7801 gtactctgcc tgcttaccgt ctccagtata cagccgctaa atttgttaac attaaagcgg
     7861 atagagcgct ctcccattga acacttcacg acctcatcat tcttcccacg gatggtgccc
     7921 cggaaagaat tatctttatg ataactgata ccaaaagagc tgaaggacac acctgtcgca
     7981 tttactggca taacaaatcc t
//
LOCUS       DAZGTR010000169         7918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGTR010000169 DAZGTR010000000
VERSION     DAZGTR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.171"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            112..723
                     /gene="ruvA"
                     /locus_tag="ACHLCY_004420"
     CDS             112..723
                     /gene="ruvA"
                     /locus_tag="ACHLCY_004420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP0371528.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            732..1742
                     /gene="ruvB"
                     /locus_tag="ACHLCY_004421"
     CDS             732..1742
                     /gene="ruvB"
                     /locus_tag="ACHLCY_004421"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP0371529.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHLCY_004422"
     CDS             complement(1889..2674)
                     /gene="znuB"
                     /locus_tag="ACHLCY_004422"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP0371530.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHLCY_004423"
     CDS             complement(2671..3426)
                     /gene="znuC"
                     /locus_tag="ACHLCY_004423"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP0371531.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3505..4437
                     /gene="znuA"
                     /locus_tag="ACHLCY_004424"
     CDS             3505..4437
                     /gene="znuA"
                     /locus_tag="ACHLCY_004424"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP0371532.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARAIAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4453..5775
                     /gene="mepM"
                     /locus_tag="ACHLCY_004425"
     CDS             4453..5775
                     /gene="mepM"
                     /locus_tag="ACHLCY_004425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP0371533.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHLCY_004426"
                     /gene_synonym="msbB"
     CDS             5895..6866
                     /gene="lpxM"
                     /locus_tag="ACHLCY_004426"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0371534.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6955..>7918)
                     /locus_tag="ACHLCY_004427"
     CDS             complement(6955..>7918)
                     /locus_tag="ACHLCY_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFP0371535.1"
                     /translation="SEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKT
                     TMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQI
                     QFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQETGIALIAKNKYGYRVKDQTLELTL
                     LRSQHKPGEVVKTDNYDNFLINNFADIGKQKFTFSLFPHHGDYRVGGVVNQAYIMNHP
                     LQIVPVKPPPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESYGLEISAMFS
                     WVCNYHYVQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2096 a         1889 c         2020 g         1913 t
ORIGIN      
        1 ttttcagatc gttgggacgc ctcgcaagtt ttcttcatcc ttcgctggat atctatccag
       61 cattttttta tcatacagca ttatctttga ttcattacgc aggagcgtca tgtgataggc
      121 agactcagag gcatcatcat tgaaaaacaa cccccgctgg tgttaattga agtgggcggc
      181 gtaggctatg aagtgcatat gccgatgacc tgtttttatg aactccctga agcgggtcag
      241 gaagcgatcg ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct gtacggtttt
      301 aacaataaac aagagcgcac attgttcaaa gagttgatca aaaccaacgg cgtcggcccg
      361 aagttggcgc tggcgatcct ctccggaatg tcagcgcagc agttcgttaa tgccgttgag
      421 cgtgaagaag tgggggcact ggtgaaactg ccgggtattg gcaaaaaaac cgccgaacgc
      481 ttgattgttg aaatgaaaga ccgatttaaa ggtttgcatg gcgatctctt tacgccagcc
      541 gccgacctgg tactcacgtc acctgccagc ccgacgaccg acgatgctga acaggaagcg
      601 gttgccgcgc tggtggcgct gggctataaa ccacaagaag caagccgcat ggtgagcaaa
      661 atcgctcgcc ctgacgccag cagtgaaaca ttaattcgcg aagccctacg cgccgcgtta
      721 tgaggtaaag gatgattgaa gcagaccgtc tgatttctgc cggtaccact ttgccggaag
      781 atgtggcaga tcgcgccatt cgccctaaat tactggaaga gtatgttggt cagccgcagg
      841 ttcgttcaca gatggagatt ttcatcaaag cagcgaaact gcgcggcgat gccctcgatc
      901 atctgttgat tttcggtcct ccggggttgg gtaaaactac gcttgccaac attgtcgcca
      961 atgaaatggg cgttaattta cgcacgactt ctggtccggt gctggaaaag gcgggcgatc
     1021 tggcagcgat gctcactaac cttgaaccgc atgacgtgtt gtttattgat gagatccacc
     1081 gtctttcgcc agtggtggaa gaggtgttgt atccggcaat ggaagactac caactggata
     1141 tcatgattgg tgaaggtccg gcggcacgct ccattaaaat tgatttgccg ccgtttaccc
     1201 tgattggtgc aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgat cgttttggta
     1261 ttgtgcagcg tctggagttt tatcaggtgc cggatctgca atatatcgtc agtcgcagcg
     1321 cacgctttat ggggcttgag atgagtgatg acggcgcgct ggaagttgct cgtcgagctc
     1381 gcggtacgcc gcgcattgcc aaccgtctgc tgcgtcgagt gcgtgatttc gccgaagtga
     1441 agcacgatgg caccatctcg gcagatatcg ctgctcaggc gttggatatg ttgaatgtcg
     1501 atgctgaagg tttcgattat atggaccgca agttattgct ggcggtaatc gataagttct
     1561 ttggtgggcc ggtaggtctg gataaccttg cggcagccat tggcgaagaa cgtgaaacca
     1621 ttgaggatgt gctggaacct tatttgattc agcaaggctt tttgcagcgt acaccgcgtg
     1681 ggcgtatggc gacgacgcgg gcgtggaatc actttggcat aacgccgcca gaaatgccgt
     1741 aagtcggatt ggtcgctcgc gccgcatccg acactggcag tgtgtctgat gcgacactgt
     1801 tcgcgtctta tcaggccagc cagagatagc gaaccgtagg tcggataagg cgctcgcgcc
     1861 gcatccgaca aatgtgttca gcgatagatt agctggcctg ctttttcatc atactgagaa
     1921 taaataacag tgccgcacat agcaccaccg acggacccgc cggcgtatcg taaaacgcgg
     1981 aaaaggttaa accgccagtt actgccacca tccccaccaa aacagcgaca ccagccatct
     2041 gttccggcgt acgggcaaag cgacgcgcag tagctgcagg aataatcagc agtgaagtaa
     2101 taatcaacgc gccaacaaat ttcatcgcta caccaatcgt caatgccgtc accagcatca
     2161 acaacaattt cacgcgctgt aatttcacac catcaacaaa cgccagatcc gggctaatcg
     2221 tcatcgacag caaattgcgc cattgccaga acaaaatagc caccacgatg accacgccaa
     2281 tcgcaatagc gatgagatct tctggcgtca ctgccagcaa atcaccgaac aggtaagcca
     2341 tcaaatcaac acgaatgtta gacatcagac taacgaccac caggcccagc gacagggcac
     2401 tgtgcgccat aatccctaat aacgtgtcaa tcgccagctg tggacgcttc tccagccata
     2461 ccagaccgcc cgccaggagc agcgtaacgg caattaccgc atagaatgga ttcacgtcca
     2521 gcaacaaacc aaacgcgacg ccaagtaatg aggcatgagc cagcgtatca ccgaaataag
     2581 acatacgacg ccagactaca aacgaaccca gcggacccgc ggcacaggcg agcatgatcc
     2641 cggctaacca accgggaaat aataattcaa tcatgagcga tcatttcccc gacgcaaaac
     2701 aattcgtccc tgtaaatcgt gacgatgatt atgatgatgg cgatagatac ccagttgttc
     2761 agcaccacga ggaccaaaca tagaaataaa ctccggatgt agggaaacaa cttccggtgt
     2821 gccggaacaa caaatgtggt gattcagaca cagcacttca tcggtttttg ccattaccag
     2881 atgcagatcg tgagagacca ttaaaacgcc acaatccagt tcgcgacgca actggtcaat
     2941 aaggtcatat aacgccacct ggccatttac atccacgcct tgagtgggtt catccagcac
     3001 taataattgc ggtcgattta acaatgctcg cgctaacagt acacgctgtg tttcgccacc
     3061 agagagcttt tgcatcggtg cgttaatcag atgcccggcc tggacacgtt tcagtgcagg
     3121 caaaatatct tctttatgtg tgccagggcg taagcgtaaa aaacggttta cggtcagtgg
     3181 caacgtggtg tcgagataca gcttctgcgg tacatagccg atgcgtagtt ttccgttgcg
     3241 cttgataacc ccttcatcgg gtgttaccag cccgagcact acccgtacca gtgtcgactt
     3301 acctgcgcca tttggcccaa gtaaagtcaa aatttttcca ggtttaagtt ccagcgacac
     3361 atcagagagg acgcggcgtt ggccaaaaga aaccgagaca ttttccaggg aaaccagact
     3421 tgtcatgtta attttagtct tgcagtagtc atgaaatgtt ataatatcac acttctcata
     3481 ttcattacga tgattggtcg cattatgtta cataaaaaaa cgcttctttt cgcagcatta
     3541 tccgccgctc tctggggagg tgcaacacag gccgcagatg ccgccgttgt cgcttcgctt
     3601 aaacccgttg ggttcatcgc ttctgccatt gctgatggtg taacagaaac ggaggtttta
     3661 ctgcctgacg gcgcttcaga acatgattat tcactgcgtc catcggatgt aaaacgctta
     3721 cagaacgcgg acttagtcgt ttgggttggc ccggagatgg aagcgtttat gcaaaaaccg
     3781 gtaagcaaat taccaggagc gaagcaggta acgattgcgc agcttgaaga tgtgaaaccg
     3841 ctactgatga aaagtattca cggcgatgat gatgatcacg accacgcgga aaaaagtgac
     3901 gaagatcacc atcacggcga tttcaacatg catctttggc tttccccaga gatagcgcgg
     3961 gctatagcgg ttgcaatcca tggaaaatta gtggaactta tgccgcaaag tcgagccaaa
     4021 cttgacgcca acctgaagga ttttgaggca caattagcct caaccgaaac gcaggttggt
     4081 aacgagctcg cgccgctcaa ggggaaaggt tatttcgttt ttcacgatgc ttacggctat
     4141 ttcgaaaaac agtttggact gacaccgctt ggtcatttta ccgttaaccc tgagattcaa
     4201 cctggcgcgc agcgtttaca tgaaataaga acacagttgg ttgagcaaaa agcaacctgc
     4261 gtttttgctg agccacagtt caggccagcg gtcgttgaga gcgtcgcacg agggacatcc
     4321 gttcgtatgg gaacgttgga tcccctgggg acgaatatca aactgggtaa aacaagctat
     4381 tcagaattcc tgagtcaatt agccaaccag tatgcgagct gcctgaaagg agattaatga
     4441 ggaagtgatt acgtgcaaca gatagcccgc tctgtcgccc tggcgtttaa taatttaccg
     4501 cgaccacacc gcgttatgtt ggggtcgctc accgttctta ctctggccgt cgctgtctgg
     4561 cggccttatg tttatcaccg tgatgccacg ccaattgtca aaaccattga gctggaacag
     4621 aacgaaattc gttcgctctt acctgaagcc agtgagccga ttgatcaagc tgcacaagaa
     4681 gatgaagcca ttccccagga cgaactggat gacaaaatcg ccggtgaagc gggcgtgcat
     4741 gaatatgttg tttccactgg cgatacgcta agcagcattc tcaatcagta tggtattgat
     4801 atgggtgata tcacccaact ggctgcggcc gacaaagaat tgcgtaacct gaaaatcggt
     4861 caacaactct cctggacatt aaccgcggac ggcgaactgc agcgcctcac ctgggaagtg
     4921 tctcgtcgtg aaacccgaac ctatgaccgt actgccgcta acggttttaa aatgaccagc
     4981 gaaatgcagc aaggagagtg ggttaacaat ctgctgaaag gtaccgtcgg ggggagcttt
     5041 gttgccagcg ccagaaacgc cggtttaacc agcgccgaag tgagcgcagt gattaaagcc
     5101 atgcagtggc aaatggattt ccgcaaactg aaaaaaggcg atgaatttgc ggtgttaatg
     5161 tctcgagaaa tgcttgatgg taaacgtgag caaagccagc tgctgggcgt acgtttgcgt
     5221 tcagaaggta aagattatta cgctatccgc gctgaagatg gcaaattcta cgatcgtaac
     5281 ggtactggtc tggcgaaagg attcttgcga ttcccgacgg cgaaacagtt ccgtatctcg
     5341 tctaacttta acccgcgtcg aactaatccg gtgacaggtc gcgttgcacc acacagaggt
     5401 gttgatttcg ccatgccaca aggtacgcca gtgctttcag tgggtgacgg tgaagtggtg
     5461 gttgccaaac gcagtggcgc agcaggttat tatgtggcta ttcgtcatgg tcgcagctac
     5521 accacgcgtt atatgcactt gcgcaagatt ctggtgaaac cgggacagaa ggtgaaacgt
     5581 ggcgaccgta tcgcgctttc cggtaatacc ggacgttcaa ctgggccgca tctgcactat
     5641 gaagtatgga taaaccagca ggccgtaaac ccgctgacgg caaaactgcc gcgtactgaa
     5701 gggctgaccg gctccgatcg tcgcgaattc ctggcgcagg ccaaagagat tgtgccgcag
     5761 ctacggtttg attaattaac atccattcgc agccggtacg cagtcagtac cggctttttt
     5821 tatttggtgc ggggcaagtt gcgccgctac actatcacca gattgatttt tgccttatcc
     5881 gaaactggaa aagcatggaa acgaaaaaaa ataatagcga atacattcct gagtttgata
     5941 aatcctttcg ccacccgcgc tactggggag catggctggg tgtagcagcg atggcgggta
     6001 tcgctttaac gccgccaaag ttccgtgatc ccattctggc acggctggga cgttttgccg
     6061 gacgactggg aaaaagctca cgccgtcgtg cgttaatcaa tctgtcgctc tgctttccag
     6121 aacgtagtga agctgaacgc gaagcgattg ttgatgagat gtttgccacc gcgccgcaag
     6181 cgatggcaat gatggctgag ttggcaatac gcggaccgga gaaaattcag ccgcgcgttg
     6241 actggcaagg gctggagatc atcgaagaga tgcggcgtaa taacgagaaa gttatctttc
     6301 tggtgccgca cggttgggcc gtcgatattc ctgccatgct gatggcctcg caagggcaga
     6361 aaatggcagc gatgttccat aatcagggca acccggtttt tgattatgtc tggaacacgg
     6421 tgcgtcgtcg ctttggcggt cgtctgcatg cgagaaatga cggtattaaa ccattcatcc
     6481 agtcggtacg tcaggggtac tggggatatt atttacccga tcaggatcat ggcccagagc
     6541 acagcgaatt tgtggatttc tttgccacct ataaagcgac gttgcccgcg attggtcgtt
     6601 tgatgaaagt gtgccgtgcg cgcgttgtac cgctgtttcc gatttatgat ggcaagacgc
     6661 atcgtctgac gattcaggtg cgcccaccga tggatgatct gttagaggcg gatgatcata
     6721 cgattgcgcg gcggatgaat gaagaagtcg agatttttgt tggtccgcga ccagaacaat
     6781 acacctggat actaaaattg ctgaaaactc gcaaaccggg cgaaatccag ccgtataagc
     6841 gcaaagatct ttatcccatc aaataaaaaa cagcctctca aattattgag aggctgtact
     6901 tgataaaaaa tctcaatgag tgttctcacc attttgagtt tttagattag tacgctattg
     6961 agttaatcgt agcgtgataa tttcaaaagg tttgaattca agattgcaat attgattgca
     7021 ataaaaactg tcatctaatt tattttcttg gagatcgaca tattgaacgt agtgataatt
     7081 acatacccac gaaaacatgg ctgaaatttc taatccataa ctctcatata atcttacgat
     7141 tataccgtcg ccgtcttcag ctaattttat tgtttcgatg ataacgctgt tagtatcgat
     7201 agcaaaaaat gatagtgaag ggggtagttc acccgggggt ggtttgacag ggacgatctg
     7261 taagggatga ttcattatat aggcctggtt aacgacacca cccacacggt aatctccatg
     7321 atgaggaaat aaggagaatg tgaatttttg tttaccaata tctgcaaaat tattaatcag
     7381 gaaattatca taattatctg tcttaacaac ttccccaggt ttgtgctgcg aacgcaacaa
     7441 agttaactct agcgtctggt ctttgacacg atagccatat ttgttttttg ctattaaagc
     7501 aatgccagtt tcttgatcag aaaggtcgac ccagtgatgt gctggtattt catctttggc
     7561 aaaagcgaac gaatcatcgc tatgcgttgg tctgtcaatt acaccgaact gaatttgaca
     7621 ttgtgcaaga gatgtttgta ctgataccgg gaattttact ttgagagaaa cattaggttc
     7681 gtgccaatca ataaaagttt ggacatcaac gcgtggactg tcctgtgtca gggtaaatgc
     7741 gaagcgaaga taagaggaat tgtagtgata aatcagcgtc atcgtagttt taggcccgct
     7801 gatcacagtt gtcatttttt cagcgagtaa aacatatgat tccccatcac gataatttga
     7861 agcaaaatcc caggccgcga aaaaacctgc atcttcgtgg tatgcacgga tctcactg
//
LOCUS       DAZGTR010000170         7779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGTR010000170 DAZGTR010000000
VERSION     DAZGTR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.172"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..192
                     /locus_tag="ACHLCY_004428"
                     /pseudo
     CDS             1..192
                     /locus_tag="ACHLCY_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            460..1089
                     /locus_tag="ACHLCY_004429"
     CDS             460..1089
                     /locus_tag="ACHLCY_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0371536.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVGKDFSNWIRARL
                     NEYGFVENLDYILFSPNLAKTPGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRQSLLPAPMNINYPLSWFSEHHPYSMMSYVDRKTLNLDVSVLFDMPSPTMRILN
                     ELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            1145..1576
                     /locus_tag="ACHLCY_004430"
     CDS             1145..1576
                     /locus_tag="ACHLCY_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP0371537.1"
                     /translation="MKQIAFYRRSGRPGAFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARGLTKGSKVVKIKASEPFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYVQKAERRRRLIKAGLYIDEFENAL"
     gene            1573..2199
                     /locus_tag="ACHLCY_004431"
     CDS             1573..2199
                     /locus_tag="ACHLCY_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFP0371538.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAAENCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            2159..2371
                     /locus_tag="ACHLCY_004432"
     CDS             2159..2371
                     /locus_tag="ACHLCY_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP0371539.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPTV"
     gene            2407..2778
                     /locus_tag="ACHLCY_004433"
     CDS             2407..2778
                     /locus_tag="ACHLCY_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0371540.1"
                     /translation="MNAVLTELNKLGKASAESISKGLNIDLNDVIDTLWKLKNQGVVT
                     VKNGIWQAVAREVDKKPNIASVQPVQPVQPVQHNIIGDLLRKSRKEARRAGRKQKRWE
                     GACKALQELNKYRDLINELSE"
     gene            3142..3393
                     /locus_tag="ACHLCY_004434"
     CDS             3142..3393
                     /locus_tag="ACHLCY_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0371541.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERIEHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            3439..3723
                     /gene="xisR"
                     /locus_tag="ACHLCY_004435"
     CDS             3439..3723
                     /gene="xisR"
                     /locus_tag="ACHLCY_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0371542.1"
                     /translation="MRELVNQHNHGIQPVITPVVQINANEWVTLELLMAITGLRKGTI
                     LRARDSAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            3776..5086
                     /locus_tag="ACHLCY_004436"
     CDS             3776..5086
                     /locus_tag="ACHLCY_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0371543.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGPGRLASSITKEDLLFIRKDLLTGEKGSRKTSTSRKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTRDEFSRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKAGTITVRRNFTKIGDFTLPKTDAGTNRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAIKRAGIRSRKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            5083..5661
                     /gene="wrbA"
                     /locus_tag="ACHLCY_004437"
     CDS             5083..5661
                     /gene="wrbA"
                     /locus_tag="ACHLCY_004437"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0371544.1"
                     /translation="MINHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            5682..5909
                     /gene="yccJ"
                     /locus_tag="ACHLCY_004438"
     CDS             5682..5909
                     /gene="yccJ"
                     /locus_tag="ACHLCY_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP0371545.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5947..7188)
                     /gene="agp"
                     /locus_tag="ACHLCY_004439"
     CDS             complement(5947..7188)
                     /gene="agp"
                     /locus_tag="ACHLCY_004439"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP0371546.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(7480..>7779)
                     /locus_tag="ACHLCY_004440"
     CDS             complement(7480..>7779)
                     /locus_tag="ACHLCY_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371547.1"
                     /translation="KARLVLDTDPEQKVNEVKNFLTALSDSICSLFIVNSNVASISLG
                     NESFSTDDDLEYGYLINTGNHYDVYLPPELFAQAYELNNKERNAQIDFLTRYAIY"
BASE COUNT         2200 a         1876 c         1845 g         1858 t
ORIGIN      
        1 ctacactctt ttcttggtgt gggcagaatg ttcgcgcact gggttaaaga gcgcattgct
       61 gaatatggat tcgttgaaaa tcaagatttt gttattgctt gccaaaattg gcaagcaaaa
      121 ggtagaggcg gtcacaaccg caaggaatac cacctcaccc tcgacacagc caaagagctt
      181 gcgatggttg acgactgcgt ccaatactga ctatactccg cgccgaggcc tcgaaaacct
      241 cccaaaagcg gattaaacca accccgtcag tgttggattt tttatgcctg ttattcagtg
      301 atagcacagt gtgcggtcac atccccgatc aatgtcggga gggcgacgaa tacaacaccc
      361 gcaaggggaa taagtccgcg gtatcttttg ggccgtttcg agcctcccgg caccacattt
      421 tgtggtggca tttctcgaaa aaagccaaag gaggtcatca tgaccactca actcatccct
      481 gtgtttgaag gtacaatctc aaatgaacct acgcttctgg tgaacgcgcg tgatttgcat
      541 ggatttttag aggtaggtaa agatttttct aactggatac gggccaggct aaacgagtat
      601 ggattcgtag aaaatttaga ctacattcta ttttcgccaa atttggcgaa aactccaggc
      661 cgccgccgca aggattacca cctcaccctc gacacagcca aagaactggc gatggttgag
      721 cgtaacgaaa aaggccgcca gatccgccga tacttcatcg agtgcgaaaa gaaacttcgc
      781 cagagccttt tacctgcacc aatgaacatc aactaccctc tatcgtggtt ttcagagcac
      841 cacccctact ccatgatgag ctatgttgat cgaaaaactc ttaacctgga cgtttctgtg
      901 ctcttcgaca tgccaagccc aactatgcgc atcctcaatg agctacacag caaaggctat
      961 aacgttgacg ccgctgtcgc tgaatttaac gccttcaagc atctgacgga agaaatgcgc
     1021 cgcacgctac aggatatttc aagattgtca gatcgaaatt cccgaaaagg cttctcgtta
     1081 agcctgtaac ttcccctacc atccccgaca tcccgtcggg gttttcatat ctggagacaa
     1141 aaatttgaaa cagatcgctt tctacaggcg ttctggaagg cccggtgcct tccgtgggtt
     1201 aaaagaacgc gtcacttgga tgattcagtc tcgtggtcgt cctgttactg gcagtgaaat
     1261 agcagagaaa tttggtgttt cactttgcga gttcaacaag gtagctcgcg gcctgacaaa
     1321 gggcagtaag gttgtgaaaa taaaggcttc agaaccattc accacagaca ccggaatcgt
     1381 tgatcgcttt ttctccctcg aatcaaatcc tcgtcgtgac acacctcgct cacgcaatgc
     1441 cgttcctcca ttcagtcgca gaagccgtga acacgcagca aaaaactgtc gcgaggaata
     1501 cgtgcaaaag gccgaacgcc gtcgccgact gattaaagca ggactttaca ttgatgagtt
     1561 tgaaaacgcg ctatgacgaa aaaatacacc ctcatttacg cagatccccc ctggacattc
     1621 cgcgacaaag caaccgatgg tcaacgcggt gcaagtttta aatatccggt catgagtctt
     1681 ctggatatct gccgcctccc ggtatgggaa ctggcagccg agaattgcct gttggctatg
     1741 tggtgggtgc ctacacaacc acttgaagca ttgaaggttg tagaagcgtg gggctttcgt
     1801 ctggtgacga tgaaaggatt aacctggaac aaatgcggga aaagacagac cgacaagctg
     1861 gtcatgggta tgggtagcac cactcgcgct aacagcgaag actgcctttt tgcagtgaaa
     1921 ggaaatctgc ccgaacgcat taacgccgga ataatccagt cattcactgc accacgcctt
     1981 gatcactccc gcaagccgga tatggctcga gaaaaacttg tgcaacttct tggcgatgtt
     2041 ccccggatag aactgttcgc ccgccacacc tcgcatggat ttgatgtatg gggtaaccaa
     2101 tgcggcacac catccattga gatggttccg ggtattgtta aatttctgga gaaaaccaat
     2161 gagcgaaaaa acgacgttga caaaggcatc accagttgaa ttaaggcagt gtctggaaat
     2221 cgcaaatcaa cttgccagaa gtggaatacg atttgttcca atcccgatta cagcagatgc
     2281 agaacttcat ctgtttggtg aaattctttc ccgaaagctg gatgaactgg aaaagctggt
     2341 agaagaagct gacacctcac caaccgtata acagccccac cgacattaaa atatcaggag
     2401 aaaaaaatga acgcagtgct cacagaattg aacaaattag gaaaagcatc agccgaaagt
     2461 atttctaaag gtctcaatat tgatttgaat gacgttattg acactctatg gaagttaaaa
     2521 aaccaggggg tagtaactgt aaaaaatggc atctggcagg cagttgcaag ggaagtggac
     2581 aaaaaaccaa atatcgcctc agtgcagcca gtgcagccag tgcagccagt gcagcacaac
     2641 attataggcg acctgctacg taaatcacgg aaagaagcgc gccgcgccgg gcggaaacag
     2701 aaacgatggg agggtgcatg taaggcgttg caagaactga ataaataccg tgacttgatc
     2761 aacgaattgt cagagtgagc gaatcacaga gagaatattt tgagaaacac cccgcgtaca
     2821 ttaatattgt ttttcctttc tctaatgacg ggcggtatcc tgttattcgc aggaacaaca
     2881 ttatttattt ttgttgcccg gctaactgcg aggataatga catgaaaatc acatttgaaa
     2941 gttacggttt aacagcaagt gtggttatat ccagctctat attcgaaagc cgcaaacatc
     3001 agcatatcgt tgatgcagtg aaactcaaag ccccggaagt taccgtcaca acacatggac
     3061 ttttcaggat acgaacgacg ttgactacac acacctctcg gcatgccgtg tttatgatat
     3121 ggcactcaag gaatacaacc agtgttcgtc ctgatccagc gcgggcagtc tttcgttgat
     3181 gccaacaact atccggtaga aatatgcaag gtaactctga ctcaggtgat ctaccgaagg
     3241 ctcgacggca gaaccagagc cacttcaatt ggtgcattta atgaagaatt tgagcgaatc
     3301 gagcacaacg aactgcatat gattaaagcg gaaattgaga aggaaaagca tattgccagc
     3361 cttcgaaaaa tgcgccgtac atcaatcaac tgacaaccgc cttcgggcgg ttttttctta
     3421 cacatgcaga ggcaacttat gcgcgagtta gtaaaccaac ataaccatgg cattcagcca
     3481 gtcatcacac ctgttgtaca gataaatgcg aatgaatggg taacactgga gcttttaatg
     3541 gctataacag gcctaagaaa aggaacaata ttacgcgcca gggacagtgc gtggatgaac
     3601 ggcagagaat ataaacaaat tgcccccgac ggaacgccaa agaaaaacag cgaatgtctc
     3661 tatcatcttc ctaccatcaa cacttggatc aaaaaccaac ccttaccatc tcaggatgtt
     3721 taattcttgt ccataagagt ataacctgag cgtgctcttg gacgcaggag gaacaatggc
     3781 gaattcagcc tatccagccg gcgttgaaaa tcacggagga aaactccgaa taacgtttaa
     3841 gtacaggggt aaacgagtgc gcgaaaatct tcgcgtgccc gatactccga aaaacagaaa
     3901 gatcgctggt gagttaaggg cttctgtctg ctttgcaatc agaacgggaa cgtttgatta
     3961 tgccgagcga ttccctgact cacctaacct gaagctattt ggcctggtaa aaaaagatat
     4021 caccgtcggt gaactggcac agaaatggct tactctgaaa gcaatggaaa tcagtagtaa
     4081 cgccttaaat cgttatcaat cagtgatgaa aaatatgcta ccgaggcttg gtcctggcag
     4141 gctggcgtca tcgattacaa aagaagatct gctgtttatc aggaaagatt tactgaccgg
     4201 ggaaaaggga agcaggaaaa ccagcacgtc ccgaaaagga agaaccgtac ccacagtgaa
     4261 ctattacatg acaacaacag ccggaatgtt cagctttgcc gccgaaaacg ggtatctgga
     4321 gaaaaacccg tttaattcaa taacaccgct gaggaaatca aaaccagtgc cggatccact
     4381 gaccagagat gagtttagcc gtctcattga tgcctgccat catcaacaga ccaaaaacct
     4441 ctggacagtg gctgttttta cagggatgcg acacggtgaa attgccgcac ttgcatggga
     4501 ggatatcgac ctgaaagctg gcacgataac agtgcgaaga aattttacaa aaataggtga
     4561 ttttacgcta ccaaagactg acgcaggcac taaccgggtt atacatcttc tggcaccagc
     4621 aattgaagca cttaaaaacc aggcgatgct tactcgtctt ggcaggcagc atcagatcac
     4681 tgttaaatta cgcgagtacg gaagaacaat tttgcacgag tgcacttttg ttttctgtcc
     4741 gcaaatcgtt cgcaagaatc acaaggcggg tattaactac gcggtaagct ccatcggagc
     4801 gacatgggat tcagcaataa aaagagcggg tatccgatcc cgtaaagcgt atcagtcacg
     4861 ccatacctat gcgtgctggg ctttatctgc cggagcaaac ccgacattta ttgcgtcaca
     4921 gatggggcac tccagcgcca gcatggtcta caatgtttat ggtgcatgga tgcctgagtg
     4981 cagcgtgact caagttgcca tgttgaataa tgtccttaat gcccgtgccc cagacgtgcc
     5041 ccaaagtgac caggaggatg aaataaaatt atatttttca aaatgataaa ccatatcctt
     5101 agacatgttg aaacgatggc acgcgcagtc gctgagggtg caagcaaagt cgatggcgca
     5161 gaagttgtcg ttaagcgtgt accggaaacc atgccgccgc aattatttga aaaagcaggc
     5221 ggtaaaacgc aaactgcacc ggttgcaacc ccgcaagaac tggccgatta cgacgccatt
     5281 atttttggta cacctacccg ctttggcaac atgtccggtc aaatgcgtac cttcctcgac
     5341 cagacgggcg gcctgtgggc ttccggcgca ctatacggaa aactggcgag cgtctttagt
     5401 tccaccggta ctggcggcgg tcaggaacaa actattactt caacctggac gacccttgcg
     5461 catcacggca tggtaattgt ccccattggc tacgcagcgc aggaattatt tgacgtttca
     5521 caggttcgcg gcggtacgcc gtacggcgca accaccatcg caggcggtga cggctcacgc
     5581 cagccaagcc aggaagaact gtctattgct cgttatcaag gggaatatgt cgcaggtctg
     5641 gcagttaaac ttaacggcta atcttcaaca ggaggatacg catgccaact caagaagcga
     5701 aagctcatca cgtcggtgaa tgggcatctc tgcgcaatac gtcgccggaa atagccgagg
     5761 ccattttcga agtcgccggg tacgacgaaa aaatggcgga aaagatttgg gaagaaggta
     5821 gcgatgaagt gttagtcaaa gcctttgcta agaccgataa agattcgctt ttttggggcg
     5881 aacagaccat cgaacgtaaa aacgtttaac cagcgactcc cccacttctc gcgggggagt
     5941 tttctgttat ttcaccgcct cattcaacac gctatcaaac ttatccattg ggcagaaacc
     6001 gttggcatct atcgggcaac cgcttaattc cagcgtcaca cgctgagcag gtgcctgcag
     6061 ggttaacgca tcggcattac gtaactgttc cgcactctga tacacatatt caattttcat
     6121 caaatcgcga ttggctttgc tgtcatgcca acgctggaaa acgattttgc cgccaatcgg
     6181 cgtgcgttcg ttctggtcat gcaactgata cggtttgaaa tccagcgccg ttaacagaga
     6241 ggcaatgttg gagtcgtgcc caaccaacac tgtaattttc ggtgcgctgg tgcgatcggt
     6301 gaccagagct ttgtcgatat aactgaccag cggtttcgca acattgcgcg ccacttccgg
     6361 tgaggtaaac aggctgtcct ggtagccgtt tttaagcttc gacaacacct tccactgctg
     6421 gtcagatttg atttctcccc aggccacctg atccatcgga aaaccttcgt aatattgcaa
     6481 agtaaacgca tctaccagcg agttgccgac tttcagcggc ccggaaacac ctggttcttg
     6541 ttgatacttg gcgctaaagg tatttttgcc atccaccagc gaacactgtt gtttctcttt
     6601 acaggcaggg gaatctttat agttaacgat tttttccagt agttggtagc tgtcggtaag
     6661 ctggagtttg ctgagctctt tctccattgc cgccaccgcc tgttcactga atgcggcgga
     6721 atcatcggtg atcaccgggt taaaggttgg gtccatagtg cccatttttt cctggtgatg
     6781 cacaggaata tcacaccccg ggaatgcgcc ggtaataaag aactgtgcgg tcgcaacggt
     6841 acgttgcaga ctattggcat aggcataaac ggtgtccggc ggcgggcatt cccccgattt
     6901 caccatcccc tgctgtgcca gccattcacg catgtaatgg cccatataca cttcgagcac
     6961 gccacctttg gtggtgagtt gcccaccggg gacgtcccat tcgggccatt tattcggcgt
     7021 agactgctcc agcacactgc cattgttcgc cagcggcgca cgtaagttat ggcggctcat
     7081 catgagcact tgctgtagct gatagccttc cggtacggtt tgtgcctgag catttgaagc
     7141 gagtaaaact atccctgcca cagttgcggc gattagcgtt ttgttcattc ctggcacctc
     7201 ttttgttatc aatcactaaa gagtgtgaca gaaatatgac atttttccgg gaacaggttc
     7261 gcaaaatggc ttgttgaatg cctgtttttc tatatacatc tttaaaaaca ggcaaggttt
     7321 gacgcaaact atgcctgatt agctccctgc ctcgcgtacg ccgctgatga ttacacctgt
     7381 ggctaaacag aatatcagcg gcgttactaa tacagaggca atagtaccaa taataagaga
     7441 agtgaggcta ataacaatgg tttttagcac atgcctttgt cagtaaattg cataacgagt
     7501 taaaaaatca atttgcgcat tcctttcctt attgtttaac tcgtaagcct gagcaaaaag
     7561 ttcaggaggg aggtaaacgt cataatgatt gccagtgttt attaaatacc catactcaag
     7621 gtcgtcatct gttgaaaagg attcattccc taaggagatt gatgccacat tactattaac
     7681 aataaataat gagcaaatgc tatctgaaag agcagtgagg aaatttttca cctcattaac
     7741 cttctgctct gggtcggtgt ctaaaacaag gcgtgcctt
//
LOCUS       DAZGTR010000171         7776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGTR010000171 DAZGTR010000000
VERSION     DAZGTR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.173"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            42..1093
                     /locus_tag="ACHLCY_004441"
                     /pseudo
     CDS             42..1093
                     /locus_tag="ACHLCY_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
     gene            1169..2602
                     /locus_tag="ACHLCY_004442"
     CDS             1169..2602
                     /locus_tag="ACHLCY_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP0371548.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWVLAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2785..5046
                     /locus_tag="ACHLCY_004443"
     CDS             2785..5046
                     /locus_tag="ACHLCY_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP0371549.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5280..6563)
                     /locus_tag="ACHLCY_004444"
     CDS             complement(5280..6563)
                     /locus_tag="ACHLCY_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP0371550.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVS
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6698..7774)
                     /locus_tag="ACHLCY_004445"
     CDS             complement(6698..7774)
                     /locus_tag="ACHLCY_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0371551.1"
                     /translation="MWCWTRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRR
                     IAITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINY
                     MSKIKAIRRGLPDAPLEDITTKEIAAMLSGYIDEGKAASAKLIRSTLSDAFREAIAEG
                     HITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDL
                     CEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIAS
                     THREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLG
                     HKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         1847 a         1865 c         1983 g         2081 t
ORIGIN      
        1 catatcgcaa taagttatgt ttttaaattg agggcattat tatgaaaaaa ataccctgcg
       61 tgatgatgcg aggtggaacc tcgaggggcg cgttcctgtt agcggaacat ttacccgaag
      121 atcaaacgca gcgcgataaa atattgatgg caattatggg ttccggtaac gatctggaaa
      181 ttgacggtat tggcggcggt aatccactga ccagtaaagt cgccattatt agccgttcca
      241 gcgatccgcg tgctgatgtt gattatctgt ttgctcaagt tatcgtccat gagcaacgtg
      301 tcgataccac gcctaactgc ggcaatatgc tttctggcgt tggggcattc gccattgaaa
      361 atggtttgat tgcagcgaca tcaccggtca cccgtgtgcg tatccgcaac gtcaatacgg
      421 gtacgttcat cgaagctgat gtgcaaacgc caaatggtgt tgtcgagtac gagggtagtg
      481 ccagaattga cggcgtaccg ggtactgccg caccggttgc gctcactttc ctgaatgccg
      541 ctggaaccaa aaccggaaaa gttttcccga ctgataatca gattgattat tttgacgatg
      601 tcccggtgac ctgtatcgat atggcgatgc cagtcgtcat tattccggct gaatatctgg
      661 gaaaaacagg ttatgaatta ccggcggaac tggatgccga caaagcatta ttagcccgca
      721 ttgaatctat ccgtctacaa gcgggtaaag caatgggctt aggtgatgtc agtaatatgg
      781 tatccctaaa cctgtgctta tttctccagc gcagaaaggc ggggcaatta atgtgcgtta
      841 ttttatgccg cattcttgcc atcgcgcgct ggcgataacc ggtgctattg ctatttccag
      901 tagttgtgca ttggaaggca ccgtcacccg acaaatcgtc ccttctgtag gatacggcaa
      961 tatcaatatt gaacacccca gtggtgcgct cgacgttcat ttaagtaatg aaggtcagga
     1021 tgccacgacg ttacgcgcat ctgttattcg gacgaccaga aaaatatttt ccggtgaagt
     1081 ttatcttccc tgaaaaaatt cgttgtcaag ataaggataa tcaataaagg acttctgtat
     1141 gagtcataca gaaagaacag gattttaaat gaataagaaa tcgttatgga agctaattct
     1201 gatattagcg atcccatgta ttattggttt tatgccagct ccggcaggat taagcgaact
     1261 ggcgtgggtg ctttttggta tttacctggc ggccattgtg gggctggtta tcaagccttt
     1321 cccggaacct gtcgtactgt taattgccgt tgctgcctcc atggtggtgg tcggtaactt
     1381 atccgacggt gcgtttaaga ccaccgccgt attaagcggt tactcttcag gtactacctg
     1441 gctggtgttc tcggcgttta ccttaagcgc cgcatttgtg accaccggtt taggtaaacg
     1501 tattgcctat ctgctgattg gtaaaatcgg taacaccacg ctgggtctgg gttacgttac
     1561 ggtattcctc gatctggtac tggctccggc aacaccgtct aacaccgcgc gtgcgggcgg
     1621 tattgtgtta ccgatcatca acagcgtggc ggtggctttg gggtccgaac cggaaaaaag
     1681 tccgcgtcgt gtcggacatt acctgatgat gtccatttac atggtcacca aaaccaccag
     1741 ctatatgttc tttaccgcaa tggcggggaa cattctggcg ctgaaaatga tcaacgacat
     1801 tctgcacctg caaattagct ggggtggatg ggtactggcc gccggattgc cgggcatcat
     1861 tatgctgctg gtcaccccac tggtgattta taccatgtat ccgccagaaa ttaagaaggt
     1921 ggataataaa accatcgcta aagcgggcct tgccgaactg ggaccgatga aaatccgcga
     1981 aaaaatgctg ctcggtgtct ttgtgctggc gctactgggc tggattttca gtaagtctct
     2041 gggggttgat gaatccactg tggcaatcgt tgttatggcg accatgctgc tgctgggtat
     2101 cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg aataccttaa tctggtacgg
     2161 cggtattatc ggattaagct ccttattatc gaaagttaaa ttcttcgaat ggttagctga
     2221 agtctttaaa aataacctgg catttgatgg tcacggtaac gttgctttct tcgttattat
     2281 tttcctcagc attatcgtgc gttatttctt cgcttccggt agtgcctata tcgttgctat
     2341 gttaccggta tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt taaccgcgct
     2401 ggcactgttg ttctccaact cctatggcgg catggttact cactatggcg gcgcggcagg
     2461 tccggtcatc tttggcgtgg gttataacga tattaaatcc tggtggttgg tcggtgcggt
     2521 actgacgata ttaaccttcc tggtgcatat caccctcggc gtgtggtggt ggaatatgct
     2581 gatcggctgg aacatgctgt aaatattctc gtcatacttc aagttgcatg tgctgcgtct
     2641 gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact cgcttgtcgc
     2701 cttcctgcaa ctcgaattat ttagagtata tccatttatt atctttctgc gcacttcacg
     2761 gtgcgcagat atctggagca tttgatgatc aagttatctg aaaaaggcgt gtttctcgcc
     2821 agtaataacg aaataattgc cgaagaacat ttcaccggcg aaattaaaaa agaagaagcc
     2881 aaaaaaggca ctattgcctg gtctattctc tcttcgcata atacgtccgg aaatatggat
     2941 aaacttaaaa ttaagtttga ttcattagcc tctcacgata ttacctttgt tggtattgta
     3001 cagaccgcta aagcgtccgg tatggaacgt ttcccgctgc cgtatgtgct gaccaactgc
     3061 cataactcac tctgcgccgt cggcggcact attaacggtg atgaccatgt ttttggttta
     3121 tcggcggccc agcgttatgg cggtattttt gtgcctccgc atattgcggt catccatcaa
     3181 tatatgcgtg agatgatggc aggcggcggc aaaatgatcc tcgggtcaga cagccacacc
     3241 cgttacggtg cattagggac aatggcagtc ggtgagggcg gcggtgagtt ggtaaaacag
     3301 ctgcttaatg acacctggga tatcgactat ccgggcgtgg ttgcggtgca tctgaccgga
     3361 aaacctgcgc cgtatgtggg gccacaggat gtggcgctgg ctatcattgg cgcggtgttc
     3421 aaaaacggtt acgtcaaaaa caaagtcatg gagttcgttg gaccgggcgt tagcgcgctc
     3481 tctaccgatt tccgtaacag cgttgacgtg atgaccacgg aaaccacctg tttaagctcc
     3541 gtctggcaaa ctgatgaaga agtgcataac tggctggcgc tgcacggtcg cggccaggat
     3601 tactgccagc ttaaccctca accgatggcg tactacgatg gctgcatcag cgttgattta
     3661 agcgccatca aaccaatgat tgcgctgccg ttccacccga gcaacgtgta tgaaatcgac
     3721 acactgaacc agaacctgac cgacattctg cgtgagattg aaattgagtc cgaacgcgtg
     3781 gcgcacggta aagccaaact ctcgctgctg gataaagtgg aaaatggtcg cctgaaagtg
     3841 cagcagggga ttatcgcggg ctgttctggc ggtaactacg aaaacgtcat cgcggcggcg
     3901 aatgcactgc gcggtcaatc ctgtggcaat gacaccttct cgctggcagt ttacccgtca
     3961 tcacagccgg tgtttatgga tctcgccaaa aaaggtgtgg tagcagattt gattggcgca
     4021 ggcgcaatca tcagaaccgc gttctgcggc ccatgctttg gcgcgggcga tacgccaatc
     4081 aacaacggtt tgagtattcg ccacaccacg cgtaacttcc cgaaccgcga aggctctaag
     4141 ccagctaatg ggcagatgtc agcggtggcg ttgatggacg ctcgttctat cgctgcgact
     4201 gcggcaaacg gtggctattt aacctctgcc agcgaacttg attgctggga caacgtgccg
     4261 gagtacgcct tcgatgtaac gccgtataaa aaccgtgttt atcagggctt tgtgaaaggg
     4321 gcaactcagc aaccgctgat ttacggaccg aacattaaag actggccgga attgggtgcg
     4381 ctgactgaca atatcgtcct gaaagtgtgc tcgaagatcc tcgacgaagt gaccaccacc
     4441 gacgaactga ttccttccgg tgaaacctct tcttatcgtt caaatccgat tggtctggcg
     4501 gagtttaccc tgtcacgccg cgatcccggt tatgttggca gaagtaaagc gactgctgag
     4561 ctggaaaatc agcgtctggc ggggaatgtc agcgagctga cagaggtgtt tgcgcgcatt
     4621 aagcagattg ctggtcagga gcatattgat ccgctgcaaa ctgaaattgg cagcatggta
     4681 tatgcggtga aaccaggcga tggttctgcg cgtgaacagg cggcgagctg ccagcgtgtg
     4741 attggcggtc tggcgaatat tgccgaagag tacgcgacta aacgctaccg ttctaacgtc
     4801 atcaactggg ggatgttacc gctgcagatg gcggaagtgc caacctttga agtgggggat
     4861 tacatttaca tccctggcat taaagcggcg ctggataatc cgggtacgac gtttaaaggt
     4921 tatgtgatcc atgaagatgc gccggtaacg gaaattacgc tctatatgga aagtctgact
     4981 gctgaggagc gcgagattat caaggcgggt agtttgatta acttcaataa aaaccgtcag
     5041 atgtaaaaag cgccatgtga atgtaggtcg cattcggcac ttattgtcgg atgcgatgct
     5101 tgcgcatctt atccgaccta cgaatcgcat cgaatctgta ggccagataa ggcattttcg
     5161 cagcatccgg cacttattgt cggatgcgat gcttgcgcat cttatccgac ctacaaatcg
     5221 catcgaaccg taggccggat aaggcgttta cgccgcatcc ggcaaatagt taattgctct
     5281 tacttcttcg cctctgcaac cactttacta cccacgccgc ggttattgta ttcccacatg
     5341 cggttgtagt tagtgtcatt cagattgcgc tgtatttcgt cgttatcatc tacgctgccg
     5401 gtattacccg caaacggacg attagagatc accgcatcgg cccacggttt agccgtgtta
     5461 aaaccttcgt tgatggcgct atcacggatc accacctgac cgttggtatt ggcatcaaca
     5521 tccagcgagc ggcccagttg cgccacacca tcaccgaaag cattgaaacg gctgtttacg
     5581 gcgaggaaac cgtagtaaat gttggacagc gtagccggtg caaacacata cgcttcttgc
     5641 tgagtacgtg agttcaccac gcggaattcg gtgttatcga acaccactgc gccgcgacca
     5701 gaaacgatat ccacatcccc ttcaatgtag ctgttgctca ccagcgtacg cggctgacga
     5761 ttcgtttcca gacggttctg cacaccgctg ttggtgacaa agaaggtgtt ctgacgaccg
     5821 agaatgttaa cgttgttaat ctgtacctgg tcaccatcag tacgcagtgc caccgccgga
     5881 tggttacctg catctacgct atcgcccagc gtgttttcga tggtcagatt ttgcagttgc
     5941 aggccattgt tttgtgacca gaagaccgca gagcagagaa caccgatact gtcgctgcgt
     6001 ttgctctggc agctatcgta catataccac gctggtttac ctggcatata tttgccgcgc
     6061 gggttgacgt cgtgacgcca gtcggcaggg ctcatgccac catcaaggga aagcccaatc
     6121 ttcacatcaa tcggtttttc acctgtaccg tacagagtaa ttccacccgg agcggcaggg
     6181 acatataccg ttccctgata ctcaccaggc atcacggcaa tatactggcg cttgttggta
     6241 cgcttgataa ttgccgcatc taccgccgcc tgaatcgtgg tatgcgttac accttgagtg
     6301 cccgccgggc cgacaacaaa gtcaggttgc gcaggcaggg taatcgggga aggattccac
     6361 gctgctgcac ctggtgtcag ggatgcaaaa tagtgttgag catcgaaatt ctgcgcttct
     6421 tttgccgaca gaatcgggcg agaagaggta ccaggcgcgg tttgatcaga aggacgttga
     6481 tcgggcgggg ttgagctaca ggcggtcagc gtcacgccaa aagccaatgc cagcgccaga
     6541 cgggaaactg aaaatgtgtt cacaggttgc tccgggctat gaaatagaaa aatgaatccg
     6601 ttgaagcctg cttttttata ctaagttggc attataaaaa agcattgctt atcaatttgt
     6661 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgatttca attttgtccc
     6721 actccctgcc tctgtcatca cgatactgtg atgccatggt gtccgactta tgcccgagaa
     6781 gatgttgagc aaacttatcg cttatctgct tctcatagag tcttgcagac aaactgcgca
     6841 actcgtgaaa ggtcggcgga tccccttcga aggaaagacc tgatgctttt cgtgcgcgca
     6901 taaaatacct tgatactgtg ccggatgaaa gcggttcacg atgagtagat gcaattatgg
     6961 tttctccgcc aagaatctct ttgcatttat caagtgtttc cttcattgat atcccgagag
     7021 catcaacatg caatgttgta gggatggcaa tttttacgcc tgttttgctt tgctcgacat
     7081 aaagatatcc atctacgata tcagaccact tcatttcgca taaatcacca actcgctgcc
     7141 cggtaacaac agccagctcc attgcaagtc ttagccaaca tggtgatgat tctgctgctt
     7201 gataaatttt caggtattcg tcagccgtaa gtcttgatct ccttacctct gattttgctg
     7261 cgcgagtggc agcgaccggg tttgttgtta tatggccttc agctattgcc tctcggaatg
     7321 catcgctcag tgttgatctg attaacttgg ctgacgccgc cttgccctcg tctatgtatc
     7381 cactgagcat tgccgcaatt tcttttgtgg tgatgtcttc aagtggagca tcaggcagcc
     7441 ccctccttat tgctttaatt ttgctcatgt aatttatgag agtcttctgc ttgattcctc
     7501 tgctggcgag gattttttcg tagcgatcaa gccatgaatg taacgtaaca gaattatcac
     7561 tgttgattct cgctgtcaga ggcttgtgtt tgtgtcctga aaataactca atgttggcct
     7621 gtattgcttc agtgattgct atcctcctgt ctcggcctaa tccaaactct ttacccgtcc
     7681 ttgggtccct gtagcagtaa tatccattgt ttcttatata aagattaggg ggtaaatccc
     7741 ggcgctcatg acttcgcctc gtccagcacc acatcc
//
LOCUS       DAZGTR010000172         7749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGTR010000172 DAZGTR010000000
VERSION     DAZGTR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.174"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1160)
                     /gene="traG"
                     /locus_tag="ACHLCY_004446"
     CDS             complement(<1..1160)
                     /gene="traG"
                     /locus_tag="ACHLCY_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFP0371552.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAELVSKSTDFLSRNPEIANLFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAK"
     gene            complement(1157..1756)
                     /locus_tag="ACHLCY_004447"
                     /pseudo
     CDS             complement(1157..1756)
                     /locus_tag="ACHLCY_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
     gene            complement(1752..1907)
                     /locus_tag="ACHLCY_004448"
                     /pseudo
     CDS             complement(1752..1907)
                     /locus_tag="ACHLCY_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2689 domain-containing protein"
     gene            complement(1846..2436)
                     /gene="traP"
                     /locus_tag="ACHLCY_004449"
     CDS             complement(1846..2436)
                     /gene="traP"
                     /locus_tag="ACHLCY_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFP0371553.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVLPGKMKCIRVVTADERLT
                     EVYTEDASLPEKIRKCPVYFPDDRTNRSNGDKNEHA"
     gene            complement(2426..3853)
                     /gene="traB"
                     /locus_tag="ACHLCY_004450"
     CDS             complement(2426..3853)
                     /gene="traB"
                     /locus_tag="ACHLCY_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFP0371554.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGES
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQNRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(3853..4581)
                     /gene="traK"
                     /locus_tag="ACHLCY_004451"
     CDS             complement(3853..4581)
                     /gene="traK"
                     /locus_tag="ACHLCY_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFP0371555.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(4568..5134)
                     /gene="traE"
                     /locus_tag="ACHLCY_004452"
     CDS             complement(4568..5134)
                     /gene="traE"
                     /locus_tag="ACHLCY_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP0371556.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFTVSQNSADASYLQLMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(5156..5467)
                     /gene="traL"
                     /locus_tag="ACHLCY_004453"
     CDS             complement(5156..5467)
                     /gene="traL"
                     /locus_tag="ACHLCY_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFP0371557.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVFFGIKKLKKGRGSSWLRDLIYWYMPTALLCGIFHNVPDSCFRQWI
                     K"
     gene            complement(5482..5841)
                     /gene="traA"
                     /locus_tag="ACHLCY_004454"
     CDS             complement(5482..5841)
                     /gene="traA"
                     /locus_tag="ACHLCY_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFP0371558.1"
                     /translation="MNAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAQGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAG
                     FAIISVFIAVGMAVVGL"
     gene            complement(5874..6269)
                     /gene="traY"
                     /locus_tag="ACHLCY_004455"
     CDS             complement(5874..6269)
                     /gene="traY"
                     /locus_tag="ACHLCY_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-bindin protein
                     TraY"
                     /protein_id="HFP0371559.1"
                     /translation="MKRFGTRSATGKMVKLKLPVDVESLLIEASNRSGRSRSFEAVIR
                     LKDHLHRYPKFNRAGNIYGKSLVKYLTMRLDDETNQLLIAAKNRSGWCKTDEAADRVI
                     DHLIKFPDFYNSEIFREADKEEDITFNTL"
     gene            complement(6368..7057)
                     /gene="traJ"
                     /locus_tag="ACHLCY_004456"
     CDS             complement(6368..7057)
                     /gene="traJ"
                     /locus_tag="ACHLCY_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFP0371560.1"
                     /translation="MYPMDRIQQKHTRQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     NTLFHESFLTQDQSAEKWLLSQRDFCELISVTEMEAYRNEHTHLNLVEDVFIQNRFWI
                     ISIQSFLNGHRNIILWQFYDAAHVRHKDSYNQKTIVSDDIRNIIRRMSDDSSVSSYVN
                     DVFYLYSTGISHNAIARILNISISTSKKHASLICDYFSVSNKDELIILLYNKKFIHYL
                     YEKAMCIINTR"
     gene            complement(7244..7627)
                     /gene="traM"
                     /locus_tag="ACHLCY_004457"
     CDS             complement(7244..7627)
                     /gene="traM"
                     /locus_tag="ACHLCY_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFP0371561.1"
                     /translation="MAKVNLYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
BASE COUNT         1961 a         2005 c         1743 g         2040 t
ORIGIN      
        1 tgcttcgcat aaaatgtcat ggcgctgttc agaatggcgt acaacattgg ccatgactgc
       61 agccacatca gggcaaacac atagcctttc aggacagaca gcgtcagttt gttgaagaca
      121 gccgccagta ccaggagcgg gaaaataccg atggcgatcc ccgtcaggat ggtctgcgtc
      181 atgggcagtg tccgcatcgc cacatgaccg atggagacat gtgccagacg ttgcttctcc
      241 atcgatgacg tggtggccag attcaccagg ctggctgagt caccattacg tgctgcataa
      301 ctggtgatcc cctccttcag cgcattaatg gtgacattct ggcggatgat ctggctggct
      361 gatttacttg agccatagaa gtaactgtaa ctgtcgccaa tcagggtact gaataacagg
      421 ttcggatcag ggcgaccacc aaataactgc tgggcataat aatgccaggt ttttccgcca
      481 ctctgtgtgt ccagattcag cttgtctttc agcgaaaccg acgcgtcctt acaggtcacg
      541 aaattattat tgctgtcata aacgccccgt agcggactgg ggcgggaaaa aatcagcgtg
      601 tagggatcag cggaggccat aagctcctcc agcgtgtatt tgtggttcag gtaaatatcc
      661 cccatcacgc agttctgcac ataatcctgg aaaagattgg cgatttccgg attgcgggac
      721 aaaaagtcgg tgctttttga taccagttct gcgccaaaca gcatccccgt tttgctatag
      781 gtgacactgt ccggttgcgt gaagatcatc tcgtaactgg ccaccattgc atgcccgata
      841 cgggtcgtca gtgaaagtgg catcgccagt ccgaccggta cattatccac ccggtgaact
      901 ttgaccaggt cactgttatc aataatctgc accgatgtgc ggacattaac cagcaggctg
      961 ataagcacaa acacggccac ccagcccagc aaatccatca cgttgtgccg ctgtacccac
     1021 atcacggcca ccgcgagaac agacaatgtg agcgcaattt tttcaatgga atcccacgtc
     1081 ctggttccca taaaggcggc aatggcgttc aggttattcc gcaaccactc accaccggca
     1141 atcacataaa cttcattcac agcgtgctcc ctccgaagtg atagttgttc tggtaacgac
     1201 tgagcatgcg ggcggaaagc tgctgacgca tgtagctcat ctgacgatcg acaaccagca
     1261 gcgcatcctg ctgtacctgc acctgtgact gaaacgccgc aatctgccgg gtggcatcat
     1321 tcatgttgtc gttaatatgt tcgataactg cttcgtcata atttcctgtg gccaccatcg
     1381 cccgggcctg ctgtatcagc tcctgaatgt actgcagcag gatgtcgtaa ccgatatagt
     1441 ccgtcagctg gtaaatcata ctgttggaaa caccgagcat ctgcgggtca accaagtatt
     1501 tgaagacggg gatggtggta ctggaaataa agcctttttc cttgtcatcc agaggcgtgt
     1561 cactgaccgc cttgttctga atgctggcca gaagtttagt aatctgagac ttcagtgcat
     1621 tatcccggct gatggtcacc ggtgtgtctg ccaccacttt caggcattta tcagaatcgt
     1681 tgcagtgata aatttttgct gtgccgcctt ccatcatggc ccggataatt gagcggtcag
     1741 tggttctggc tgacaattct tgtggcgcag ttactgagaa cagcatgaat aaaatcatct
     1801 gacacggttt tacccggcac agagagggct gtaataacat taatattacg catgttcatt
     1861 tttatctccg ttgcttctgt ttgttctgtc atcaggaaaa taaacagggc atttcctgat
     1921 tttttccggc agggaagcat cttccgtata aacttccgtc agacgttcat ctgcggttac
     1981 cacccggata catttcattt ttccgggcag caccggatat ttcccggtga tcgctgccag
     2041 tgagacatac atcaatgcca gtgttgccca taatgcggtg accagttgca tcagtgaccg
     2101 gtcagtttcc ctgacataat ctgcagcatc agccgcgcgg taatggcaga tatcctgtat
     2161 ttctgatctg cctgaagaat cagaggaaga aaaccagcat tcactgaccg ggaactgtcc
     2221 ggcaggtgct gtttgtctta cgaagttaat ctccttaacc agtaatttcc ccggcgtggt
     2281 gttgtccttc cataaaacaa acagagccat cagggccatt gtggccagtg gcagaatgac
     2341 ggtgtacttc agacaccaga acagtcctcg cagaacccgg gcgacaacat accgggcggc
     2401 atggcacgcc tgacgggacg acatattatt tgccatcgtt gcctcctccc tggctgccgg
     2461 acataaactg ctgcggattc aggttgccca gctgcttcag catctggtca gtattaaagc
     2521 cgttaagatg actttcagct gatggcggaa caggaaccgg actttccgga ttatcttctt
     2581 ccgctctgtt ctgcgtgcgc tccagcgcca tctcttccac ggttttcagc tggaagccgt
     2641 cctggaacac cacggtcact tcgttgcccg caccaatcgg tatcaccggg tgatactgtt
     2701 cggcacgttt gatgtagtag tcactgagcg tctgtgcggc tttcgatgcg ccgccaccga
     2761 tacccatttt caggacatcg ccggccccgt aagcagcagt ggcacccagc ccgacagccg
     2821 gctgggaggc gcgctccatt ccctgaccga taccgtcaac aaatcccgcg ccccatgccc
     2881 agccgaggat tttgccgtta cgcatcacca cttcgccctt gataccgttt ttaccccgga
     2941 agctgacatg ccccttaatc ggcatatcaa tagttttgcc gtccttcagg cagctgatat
     3001 tgcgggtgcg tacaatggca cgttcactgg acacatcccc ccaggcttcc agacccacaa
     3061 aacatcccgt tgcgtcatac gttttgctgt tcggcatttc caccagaccg gtgatacgca
     3121 gctgcatcgg caccgtggat tcattaccgg tgactgaggc attggcatcc gctccttcaa
     3181 tcagcatggc tttcgcaaaa cttcctgacg gaatgtacgg cagtgatggt ccctgttttc
     3241 cctcattacg ggtgaacacc ttacgctgta tccggttagg caccggtaca gactggtacg
     3301 tcacctgcgg tggtggcgtg acaccattcc cgggataaaa cgccgtcggc ggtggaacag
     3361 caacgctgcc ctgcggcggg aaagatacgg gtgtgtttcc tggctgtggt tcgccttccg
     3421 ggccgggcgg tgaggctggc gtctgcggta ctggctcacc ggtcgcggtg acgggattag
     3481 cacccagggc ttttacctgc tctgccaggg cggcattgtc ctgtcccaac ttttcaatac
     3541 gacgctggtc atcaccgcgc tgtttgttca gaacatccag ctcacgacgg atttcctcat
     3601 actgcttctg catctgcgct gccgtcacct gcatctcggt ggtggcatgc tgacgcacct
     3661 tgtcatcaaa ggtcgtatcc accacaccgg tcatatccgg cacaggctcc tgttcagcca
     3721 cgctttcacc gttaccggac atgtccacat cagacagata cagtgcccca ccaatcgcgg
     3781 aggctgtacc gacaaccaca atccccagcc acaggtactg cttgcgtttc acaatggtat
     3841 tgatactggc catcagttgc cctccccgtc acggatgaca tacacatcca tgcgcgcacc
     3901 tgccagtaac tggctggcag gctggctgaa catcacggca cgggttcccg gctgccagaa
     3961 gtcactttcc cggatattca gggcagacag cgttttattt tccacggcaa agcggaccat
     4021 cttcaggtga ttccccgtcc atacggcatc tgccactgaa gacagccccg ccggggcctg
     4081 cagggtttcc tttgtcactg ggacctgata ccagcctgcg ggtaattttc cgccacggac
     4141 ggcctggctg atggtcacaa gcagggattc gtaaggcgtg gacgtttccc acgcaccggc
     4201 ttcttctccg gttccgcgca ggtcactgac cagctgaatg gtacgccccg cgccttcacg
     4261 gggaacggcc tgaatggaaa gattcagacc acgttctgtt tccagaatga acgtaaaggg
     4321 ctttttgttg acggtggcca ccaccacacc gccggaggcg gtctgctcat tattggtcag
     4381 ggcaccatcc agactgttca cggcgataat ccggtcaccg ggaacggtaa acagattcgg
     4441 gctggtattg ctgatggcaa tactggcctg accaccgttc accattggca ccacggtggg
     4501 ggccagcgta ccgtttgcgg ccatcacgct gcaggaaaaa aacaggctgc cgaatattat
     4561 tgccgtatta ttttttctca tcgtctgttt ccccgaaatt atccagccag gtcaccccgt
     4621 tttcccgttt cagaataagg atgtaatgtt tgatatcagt gaaaggtttt gaatcaccaa
     4681 tccaggtttt aagcacacca cgaatttcca cacggccata ctgaggccag acacgaacac
     4741 tggtctggaa aaaggctgag ttcacgttat cgtttttaat acgcttcgct tcttcagcca
     4801 gaataacttt catctggttc tgtgcgcccg ggcggatata ttgcagaagc gcctgatgtg
     4861 aggcatcgac ggtttctgat gaaacattca gacggagggc aataaatgat agcgccatca
     4921 gctgtaaata agaggcgtcg gcactgttct gtgacacggt aaagggggca ttaaatgcca
     4981 ttggcgtcac ggcagtgcgt tgctcattct gaagacggta gttattcacc ccctgaatga
     5041 cgttaacaga gaggctgaga acaataagca ctgacataaa tataaaggcg atggccatta
     5101 cacgactggt acttaaacgg gcaccgtgtt ccatataaca atcctggtat cagttctatt
     5161 taatccactg ccggaaacac gaatcgggaa cattatgaaa aataccgcac agcagggctg
     5221 ttggcatata ccagtaaatc aggtcacgta accaggaact gccccgccct tttttcagtt
     5281 ttttaatccc gaaaaaaacc agaaccgctg caccaatacc gaacagatat ttcgatgttg
     5341 tgatacccca gccaatacag attgctgcgg ggatcagttc atccagtggc aggccaaacc
     5401 agcggctctg gttggtcagt gtttccggga aacgatattt cttaagttta ttctcgtctc
     5461 ccgacatcgt tttatttcct gtcagaggcc aacgacggcc atacccacag caataaatac
     5521 agagatgatg gcaaaaccgg ccaggaactt gacgtttttg gtcatcatgt acatgacagc
     5581 accgaccaga acttcagcca gaacgaccca tttaacaaca ctggagtcct taccgaaggt
     5641 cgccttaacc gtggtgttac cgcttgccat caggtcctgg ccctgtgctg ccattgccag
     5701 ttgcgggaag aacatcatca gaacagcagc cgggatcaca gcgcgagcca ggcgaagcat
     5761 attcagacga gtgaatttag aaaaaaacga cttctttttg acgggcgcag aagcaccctg
     5821 aacacttaaa acagcattca taagttacct caatttcgga taaatgaata aaactagagt
     5881 gtattaaatg ttatatcttc ctctttatct gcctccctga atatctccga gttataaaaa
     5941 tcaggaaact tgatcaaatg atcaataact ctgtctgcag cctcatctgt tttacaccat
     6001 ccactacgat ttttggctgc aataagtagc tggttagttt catcatccag acgcattgtc
     6061 agatacttaa ccagcgactt accatagata ttccctgccc tgttaaactt tggatagcgg
     6121 tgaagatgat ctttaagtct tattactgcc tcaaacgatc gacttcttcc gcttctgtta
     6181 cttgcctcaa ttaatagact ttccacatct acaggtaatt ttagttttac catcttacct
     6241 gttgcagaac gtgtaccaaa tcttttcaat aacacctccc gctttttatc ttctggttac
     6301 tacttatgtt tgcagaataa tcagaaaagg taaatagacg gaaagtctat attttattaa
     6361 cactttatta acgcgtattt atgatacaca ttgccttctc gtacaaataa tgaataaact
     6421 ttttattgta gagtaagatg attaactcat ctttattaga aacagagaag tagtcgcata
     6481 tcagagacgc gtgtttcttt gatgtggaga tggatatgtt taatattctt gctatagcat
     6541 tatgactgat tccggtgcta tataagtaaa agacatcatt tacatatgat gatacagaag
     6601 aatcatcact cattcttctg attatatttc tgatatcatc actgacaatc gttttttgat
     6661 tataactgtc tttatgacga acatgagcag catcataaaa ttgccacaga atgatatttc
     6721 tgtgtccatt aagaaatgac tgaatagata ttatccagaa tctgttctga ataaaaacat
     6781 cctctacaag attaagatgc gtatgctcat tcctgtatgc ttccatctct gtgacagaga
     6841 tcaattcaca aaaatctctc tgcgacagaa gccatttttc agcactttga tcttgtgtaa
     6901 gaaatgactc atgaaacagc gtattgcaaa aaataaattt tccagtttcg tccctgatac
     6961 aagctggatt tggataatcc tgaataactg ccgtcagatt ttccagcaga tctatttgac
     7021 gagtatgttt ttgttgaata cgatccatcg gatacatagg aacctcctca caaaggattc
     7081 tatggacagt cgatgcaggg aggagagaac tccctgcatc ggcgattttc aaattcaaat
     7141 tttaacgtgg cattaaccac gtaattaata tactaattat ctttcgtact gtcacctttt
     7201 taaatcataa aaactctgat atttgaacga agtcaaattt cttttattca tcatcatttt
     7261 ttggaaaaaa tcgttccatc tcagatgata ccttctccct gatatcttca accatattgg
     7321 catattcaaa ctttggattt ccggagacat gaggactgag agactcaata cccaaaattt
     7381 tcgctactga tgattgtgtt tttacaacgc attcaagaag caatttatta aactcagtct
     7441 gattaaatgc agactcttta cgctccatct gagcctcatg tacacgaagc cccagttcaa
     7501 gaagcattga agctgttgct gaaaaactga catctttttc ccttgcccct tcctgtcgac
     7561 gcttctcaat aatcgcattt attttttcat aggcatcatt gctgatatac aggttcacct
     7621 tagccattag agattacctt ttttgaacat aaaataaagt taatttcgaa acactaaaat
     7681 tcataatgca aacagggacg caccgctagc agcgccccta gcggtatcct ataaaaaaac
     7741 acaccgcgc
//
LOCUS       DAZGTR010000173         7747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGTR010000173 DAZGTR010000000
VERSION     DAZGTR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.175"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..449)
                     /locus_tag="ACHLCY_004458"
     CDS             complement(<1..449)
                     /locus_tag="ACHLCY_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFP0371562.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLF"
     gene            complement(706..1341)
                     /gene="msrQ"
                     /locus_tag="ACHLCY_004459"
     CDS             complement(706..1341)
                     /gene="msrQ"
                     /locus_tag="ACHLCY_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP0371563.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYTLLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(1342..2346)
                     /gene="msrP"
                     /locus_tag="ACHLCY_004460"
     CDS             complement(1342..2346)
                     /gene="msrP"
                     /locus_tag="ACHLCY_004460"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP0371564.1"
                     /translation="MKKKQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(2455..2868)
                     /gene="hiuH"
                     /locus_tag="ACHLCY_004461"
     CDS             complement(2455..2868)
                     /gene="hiuH"
                     /locus_tag="ACHLCY_004461"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP0371565.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDNDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3001..3672
                     /gene="hprR"
                     /locus_tag="ACHLCY_004462"
     CDS             3001..3672
                     /gene="hprR"
                     /locus_tag="ACHLCY_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP0371566.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3672..5030
                     /gene="hprS"
                     /locus_tag="ACHLCY_004463"
     CDS             3672..5030
                     /gene="hprS"
                     /locus_tag="ACHLCY_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP0371567.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELEPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAVRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(5138..5989)
                     /gene="hchA"
                     /locus_tag="ACHLCY_004464"
     CDS             complement(5138..5989)
                     /gene="hchA"
                     /locus_tag="ACHLCY_004464"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP0371568.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            6136..6546
                     /locus_tag="ACHLCY_004465"
     CDS             6136..6546
                     /locus_tag="ACHLCY_004465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371569.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSEMRGGGERFSDVEYCGA
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            complement(6580..7743)
                     /gene="ompC"
                     /locus_tag="ACHLCY_004466"
     CDS             complement(6580..7743)
                     /gene="ompC"
                     /locus_tag="ACHLCY_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0371570.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMT
                     PYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDL
                     VNYVEIGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         2133 a         1636 c         1801 g         2177 t
ORIGIN      
        1 tcgaataagt aacgaacgcc tttcttgcct gatttatagg tgagtatttt gtatccatcg
       61 taatcatatt tacaggatgt tgtttcatta tttctatgga attcaacaat gccgtcctca
      121 atgccaatca tctcgatatc tgttgcataa cctttgtgat aataatcttt aatttcagca
      181 aatgttttag ttttatctgc atccgctttc ttctgaaaga cggggtcaag tttgccactt
      241 tgcagtaaag gataaacgga ttgccagact ccatcccagt cactgagcgt tcggttttgt
      301 acattggcat catcaaaaac accattagca gctttttgtt cgacctctgt taagggtttg
      361 ccgtgtgagt gatgaccatg cgaaaaggca ggagcgctaa caataaagac acctaaagca
      421 acagccagtt tgtgaagacg aatcgccaaa atagtttcct ccagagtcat tgtatatgat
      481 gtgtaatgtt atattgtaac atatggcaaa atgcaacaac tcttaggaga tgagagcata
      541 gttttctcag gtgtaattat ctatcttatt gaaataaaat gaattatatt gataatttga
      601 ggttgatgac aaacgaaaca taacctgatg tgatctgcgt aggccggata gggcgctcgc
      661 gccgcatccg gcagggataa cgcgtacttt gtcggcaata tgaagttata cagataattt
      721 gttgtgaacc tgcttgcgta accggttaaa aagggaacgc aacttcttat accgtaaggc
      781 taaaagcagt acagccagcc cagcgtagat gagcggttgc ggtgaaataa tcttcactga
      841 ccacagataa tgtattgggg ccaggatcgc gacaagatag acgaaattat gcaactgttg
      901 ccagtgcttg ccgagttttc gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa
      961 aataacccag ctgataattc ctaacgttaa ataaggccgg gtaattaact ccttgcctaa
     1021 taacgccaga ttattcacgc ccagctccag caatgtgtaa ctggttaaat gcagtgtcgc
     1081 ccaggcaaag caccataagc ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc
     1141 gtagcgtgcc agaggtgtga ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc
     1201 ggtaaaatgc tgaatatctt tcaccggatc ggcacccagt ccaccgtgat tgatcgccca
     1261 gaccagccag agaaacggca acaatccggc aagatgcagg ctaactttca gccatgtcac
     1321 ctgttttgct gtcagacgca ttcagaaatt ctcccgcaaa tccaggccac gatacagcga
     1381 tgccacctgg tcggcgtaac cattaaacag tagcgttggc tggcgctgaa catcgaggat
     1441 gccgcctgaa ccaataaatc gttcggtagc ctgtgaccag cgcgggtgat caacatgcgg
     1501 attaacgttg gcgtaaaaac cgtattcgtc aggcgctgcc agattccagg tggttggcgg
     1561 acgctcgcgg gtcagcttaa tactgactat cgatttaatc cctttaaagc catatttcca
     1621 cggcacaatc agtcgtaccg gcgcgccatt ttgtggcggt aacgccttgc catagacacc
     1681 cacggtcatc agtgtgagcg gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg
     1741 atatttcagc ccgccgccga taaagcggtc ctgctggcct ggcatttgtt ccggtgcata
     1801 aattgtttcg aaagcgacat acttcgcatt gctggtgggt tcggcaagcg ccagcaattt
     1861 gtgcagcgga aaaccaatcc acggcaccac catcgaccat gcctccacgc agcgcatacg
     1921 ataaatacgc tcttccagcg ggaagcgacg ggttaaatca tcgtgatcga gggtcaatgg
     1981 ttttgccact tcgccgctga ttttcagtgt ccatggatcg gttttcaggc taccagcatt
     2041 agcggcggga tcggctttat ccagcccgaa ttcatagaag ttgttataac cggagacttt
     2101 atctactggc gtcagtggca ggttattttg ccaggcggca ggcttgctga actccagcgg
     2161 ttttccggcg ggggcgggcg ggcgatcgtt ccctttaaac cagctaagca gatcggcatg
     2221 cgcagcgtga ggcaaagaaa gtgcagctgc gctgatgccc agtgctttta acacctgtcg
     2281 acgcttcata aagaataccg actcggccgt aacatctgat tcttttaaaa attgcttctt
     2341 tttcataagc catcacactt ttttgtcgtt agccagcaag agccgtttac tgttaatctg
     2401 tcagggagtg tcctccctaa cagtagcaga gatctgcttt tacatattta tgcattaact
     2461 gccacgatag gttgaatacc catattggct aagtaataaa ggcacatgat aatgctcgtt
     2521 cactttatta atatgaaatt caacggggat ctcagggaag aaactttcaa gattttgttt
     2581 cttgaaatag tccccggttt taaatacgac acggtaatcg cccgtagttg cagtttgctc
     2641 gggccacagt gccttaattc gtccatcatt atctgttttg gcggtattaa gttgtaacca
     2701 gccgttgtcc gctttctttt caagagtgac tgtcacgtcg gcagcgggtt ttccggtttg
     2761 ctggttcaaa atgtgcacgc taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa
     2821 tgaaaatgct gccgttacta cggagagtac taaataacgc tttaacatgt ttatatcctt
     2881 gtcatgtgaa tgagttttga ggatagcgaa aaagcggcca gggtaacctt acagcagcat
     2941 tacaattttg taatgaacca ggctgtttct ataacatatg atttatggca tattattttc
     3001 atgaagattt tacttattga agataatcaa aggacccagg agtgggtaac gcaggggctt
     3061 tccgaagcgg gttatgtcat tgatgccgtt tctgatggca gagatgggct ttatcttgcg
     3121 ctgaaggatg attatgcatt gatcattctg gatattatgc ttccgggtat ggatggctgg
     3181 cagatcttac aaacgttaag aacagcaaag caaacccctg ttatttgcct tactgcaagg
     3241 gattctgtcg atgacagagt cagagggctg gacagtggag caaatgatta tctggtaaaa
     3301 cctttttcat tttctgagtt gctggcaagg gttcgggcac aattaaggca acatcacgct
     3361 ttgaattcaa cattagaaat cagtggctta agaatggact ctgttagtca aagtgtgagc
     3421 agggacaata tcagtattac actgacgcgc aaggagtttc agttactttg gctactggcc
     3481 tccagagctg gcgaaattat acccagaacg gttattgcga gtgaaatttg gggaatcaac
     3541 tttgatagtg ataccaatac ggtggatgtc gccattcgca ggctccgcgc aaaagttgat
     3601 gatccttttc ctgaaaagct aatcgccaca atccggggga tgggctattc attcgtagcg
     3661 gtaaaaaaat aatgaaaaga ttatctataa ccgtccgttt aaccttgctt tttatattgc
     3721 tgctgtctgt tgctggcgct ggaattgtat ggactctcta taatggcctg gcaagtgagt
     3781 tgaaatggcg cgatgataca acactcatta accggacagc gcagatcaag cagttgttaa
     3841 ttgatggggt aaatccagat acgttacctg tgtactttaa ccggatgatg gatgttagtc
     3901 aggatatctt gatcattcat ggtgatggca tcaataaaat tgttaaccgg acgaatgtca
     3961 gtgatgacat gttaaataac atacctgcta gtgagacaat cagcgcagct ggcatttaca
     4021 gaagcattat taatgatact gagattgatg ctttacgaat taatattgat gaagtttcgc
     4081 catcattaac ggttactgtg gctaaattgg cttcagccag acataacatg cttgaacagt
     4141 ataaaattaa tagcattata atttgcattg tcgccattgt actttgctca gtattaagtc
     4201 cgctgttaat cagaacggga ttacgagaga tcaaaaagtt gagtggtgta acggaagcgc
     4261 tgaattataa cgatagccgg gagcctgttg aggttagcgc attaccgaga gaactagaac
     4321 ctcttgggca ggcgttgaat aaaatgcatc aagccttagt caaagatttt gaacgcctaa
     4381 gtcaatttgc tgacgatctc gctcatgaac ttagaacgcc cattaatgca ttactgggtc
     4441 agaatcaggt tacgctcagt caaaccagaa gtatcgctga atatcaaaaa acaattgccg
     4501 gtaacattga agagctggaa aatatttcgc ggttaacaga gaacatactg tttcttgccc
     4561 gggcagataa aaacaatgtt ttggtgaaac tggactcgct ttctctcaat aaggaagtcg
     4621 aaaatttgtt ggattatctt gaataccttt cagacgagaa agagatttgc tttaaggtcg
     4681 agtgcaatca gcaaatcttt gcggataaaa ttttactgca acgaatgtta tcgaatctta
     4741 ttgttaatgc cgttagatat tcaccagaaa aatcgcatat tcatataacc agttttcttg
     4801 ataccaacgg ctatcttaat attgatgtcg ccagtcctgg aacgaaaatt catgagcctg
     4861 aaaaactctt ccgtagattt tggcggggag ataattcgcg tcattccgta ggtcagggac
     4921 ttggtctttc tttagtcaaa gcgattgccg aattacatgg gggaagtgct acgtatcact
     4981 atctcaataa gcataatgtg ttccggatta tgttaccgca aagaaattaa catagggctt
     5041 cagtacgcca taaatcaacc atgaaaaaac gcagccacgt cgtgagtact aacgcggctg
     5101 cgattgatta tgcgcttaca ttcaaacgta acagggatta acccgcgtaa gctgccagca
     5161 tttcctgcgc cgccagttta cccaacgcat tcgctgcaaa aggactgtcg ccggtgagaa
     5221 ctttacggtc cttatgtact cgcccggtga tgtcgtcatt aatgatgttc atgcccattt
     5281 tcttcagttc ttcgccgaag taccaggtga gatgacccgg catatagcca atctctggcg
     5341 tttgtttgtc tgcggcgtct gggaatgcgc aaatggaata accattcagt gggttatcgc
     5401 cgtggcgaag cgccagaaaa gccgccgggc cgtggcaaag ggagataaca aaacggtcat
     5461 ttttgattgc ccactgcaaa gcagcagcta cgtcctggct ttctggtaaa ccaataagtg
     5521 cgccatgacc accaggaaca aagattgctg catattcgct atcagcgttg aggctggcga
     5581 caacatccgc gagtttcttc ggattgcgga acaacgattt atgttgctca aagaatggca
     5641 ttactttttc atctttgtgc ggcatagccc agtattcaaa cttggtcatc agaccggaaa
     5701 tggtcgccac ttcgaattcg aaacctgcag catggagatg atacaacggc agcaacgttt
     5761 caatcggatg gttaccggtc gagaacagtt ttccgttatc ggttggcaaa taacgttcgt
     5821 ccgcggcgat caccagaatt ttatgcttac cgcgatacgg ttttggatag tccacgccat
     5881 caagatcaga gacgggactg gtatattggc taagcgaata ttctgaaggg aagaacgcat
     5941 tatcttcagc aatatcgacc tgcggatttt tacttgtttg aacagtcata gtgtattcct
     6001 tattgttgct tagttagggt agtcactata tttgcgactg agctggtcgc tatttttaac
     6061 caggctggaa aattgagtca atatatcgaa agcggtagtc ggcgggtgta agtatgtaat
     6121 gatgatgctg cactggtgtt gaatattatt cacgccaaat tttccttatt gagcaacgtc
     6181 gtcgcaagtg gcctcaatga ttttagacca ttatcagaga tgcggggcgg tggggagaga
     6241 tttagtgatg tggaatattg tggtgctgat gaatatttat atcgcggatt ggtagagaaa
     6301 agattatttc atgcactatt tttgcaaata tttctatatg ggaagcgtgt ttgttacgga
     6361 ttaattcata aagaaatgca tttgggtgtt ggtttgaaga gtcgctggtt gatgttattg
     6421 tgccaggagg ggcagggtaa ggtgctgtat cgatatgcat tggcaattgc gtgtgccgca
     6481 gatggagatg aaatgccaat aaaaaagcct gaattcgatg agggacgaac tcaggctagt
     6541 gtttagtttt acagtaacac gccgcagcac gttggctgat tagaactggt agactaagcc
     6601 cacaccaaca acgtcatctg ttgcgatgcc attgttgtca tagaaatcgt catctttatc
     6661 caacaagttt attttatagt caacatatgt tgagaagttc ttattgaaat agtaggtcgc
     6721 tccaatttca acatagttga ccagatcctg atcaacatac tcttgatgac cattgttgtt
     6781 atagtcgtta ccgccgaggt ctttgccttt tgaataaaca tatgcaagag atgggcgcag
     6841 accaaaatcg aactgatact gtgctacagc ttcaaagttc tgggttttat ttgcgatacc
     6901 cacattacca tatggtgtca tattgcgggt ttcagcatac atcattgcaa gataaacatt
     6961 gttggcgtcg tattttgcac caaatgtcca tgcttcagct gtatcaccac cagcaaattt
     7021 gctgtattca ttgtagtgat acccgccaag agctacttga gaattggtac ggtcagagtt
     7081 tgagtaagcg gcacctaagc tcaaaccgaa gtcaaagtca tatgaagtgg aaagaccaaa
     7141 accatcaccg ttctggaagc gaacgtcttc gtgaccattt ttagtacctt cttgatcttc
     7201 tcctgcattg ctgccttcgt tgttaccttg atactgcaat gcaaagttca gaccttccac
     7261 cagaccgaag aaatcactat tgcggtaagt agctacccca tttgcacggc ctgccatgaa
     7321 gttatcagtc caggtatagg agtcgccgcc gaatacagga agcatatctg tccaggcttc
     7381 aacgtcgtaa atcacaccat agttacgacc atagtcaaag gagcccatct cgccgaactt
     7441 cagacctgca aaagcaagtt tgacataagt attatcagaa ccttcagtgt cgctagcctt
     7501 tgttttggtc agtgcttgac cataaccagt taactgatct gtaatttggg tctcaccttt
     7561 gaagcctaac tcgatgatag tttcatcgcc gtcatcgcca gggttatcgg aaaaagtatg
     7621 acgagcatca acttttccgt acagatccag tttattgcca tttttattat aaatttcagc
     7681 cgcatttgct gcgccagcaa ctaataacgc cgggaccagc attgccagaa cttttctttt
     7741 cattatg
//
LOCUS       DAZGTR010000174         7650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGTR010000174 DAZGTR010000000
VERSION     DAZGTR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.176"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..166
                     /gene="hemP"
                     /locus_tag="ACHLCY_004467"
     CDS             <1..166
                     /gene="hemP"
                     /locus_tag="ACHLCY_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP0371571.1"
                     /translation="TPETDANHKTASPQPIRRISSQTLLGPDGKLIIDHDGQEYLLRK
                     TQAGKLLLTK"
     gene            complement(170..1606)
                     /gene="selO"
                     /locus_tag="ACHLCY_004468"
     CDS             complement(170..1606)
                     /gene="selO"
                     /locus_tag="ACHLCY_004468"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP0371572.1"
                     /translation="MTLSFITRWRDELPATYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(1669..2382)
                     /gene="ydiV"
                     /locus_tag="ACHLCY_004469"
     CDS             complement(1669..2382)
                     /gene="ydiV"
                     /locus_tag="ACHLCY_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP0371573.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(2628..3092)
                     /gene="nlpC"
                     /locus_tag="ACHLCY_004470"
     CDS             complement(2628..3092)
                     /gene="nlpC"
                     /locus_tag="ACHLCY_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0371574.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(3170..3919)
                     /gene="btuD"
                     /locus_tag="ACHLCY_004471"
     CDS             complement(3170..3919)
                     /gene="btuD"
                     /locus_tag="ACHLCY_004471"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP0371575.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(3919..4470)
                     /gene="btuE"
                     /locus_tag="ACHLCY_004472"
     CDS             complement(3919..4470)
                     /gene="btuE"
                     /locus_tag="ACHLCY_004472"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP0371576.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(4533..5513)
                     /gene="btuC"
                     /locus_tag="ACHLCY_004473"
     CDS             complement(4533..5513)
                     /gene="btuC"
                     /locus_tag="ACHLCY_004473"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP0371577.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            complement(5703..6098)
                     /locus_tag="ACHLCY_004474"
     CDS             complement(5703..6098)
                     /locus_tag="ACHLCY_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371578.1"
                     /translation="MEKTVNSDLHLGQYWRIDIVCDPELRDEVGQYFSLHDVGFSKIE
                     VFSVENPYKLALFFDFAKKGVEVAKAIMGLLDRNDIEITMYRATDSSPQSVKSIKLRK
                     SEDVEKCKDLLSTCVAIGVQRNKEKEGSE"
     gene            complement(6109..6900)
                     /locus_tag="ACHLCY_004475"
                     /pseudo
     CDS             complement(6109..6900)
                     /locus_tag="ACHLCY_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247208.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            6842..7087
                     /locus_tag="ACHLCY_004476"
     CDS             6842..7087
                     /locus_tag="ACHLCY_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0371579.1"
                     /translation="MLCPDFISWPWYFHHKSISTWLIEKAGIVVHRCQRNPFTGNRIF
                     ALSNGDNQFGQDFTLYEALRTVDRLLRGQSFIKQTDL"
     gene            7098..7376
                     /locus_tag="ACHLCY_004477"
     CDS             7098..7376
                     /locus_tag="ACHLCY_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371580.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHGIISEAEKARKINGDEPYIEIICMDSEAEIQKAD"
     gene            7388..7630
                     /locus_tag="ACHLCY_004478"
     CDS             7388..7630
                     /locus_tag="ACHLCY_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0371581.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
BASE COUNT         2043 a         1998 c         1730 g         1879 t
ORIGIN      
        1 cacgcctgaa acggatgcca atcacaagac cgcttccccg cagcctattc ggcgaatttc
       61 cagccagaca ctgttaggtc cggatggcaa actgattatc gatcatgacg ggcaagaata
      121 tctgctccgt aaaactcagg ctggcaagct gctgttgacc aagtagcctt taactcgagc
      181 agctgacttc cagccgttta ccccagtcag gtggacgact gacatagtca tcagctctgt
      241 cgctgaaagg atttcgcaac gcctcatgca ggcggtgcaa ttccgtcata tcaccctttt
      301 ctgccgcttc aatcgcccgt tgcgccaacc agttgcgcaa caccagagct gggttaacgc
      361 tttgcatcag ctgctgacgc tcactatcgc taacctcgtc ttgttgcaaa cgcccccgat
      421 aacgggcaaa ccagtcatca aatgccgcac gatcaataaa ctcatcacgt agcggtgacg
      481 ccgcgctgtg ctgctcggtc agactcagca tgcggaatgt gcgggtataa tcgctgcgct
      541 ctcgcgccat cagactgaat aattcattca gtagcgcgtt atcctctttt tgctccgtca
      601 tgaagcccag tttctgccgc atccgttgtc cataatgcgt caacaaaacc tgctgatagc
      661 tgtccagggc ctcattcagg gcatctacgg caacaaatgg tgacaatgtc tgcgccagac
      721 gctgtaaatt ccacaacgcg acagcaggtt gattatcaaa gctataacgc ccttgatgat
      781 ccgagtgatt acaaataaaa ccgggttcgt aatcatccag aaaaccaaac ggcccgtaat
      841 caagcgtcag ccccagcagc gacatgttat cggtattcat caccccatga gcaaagccga
      901 ccgtctgcca ttgggcaatt aacgatgcgg tacgtgcgac aacatcgcta aaccagaggc
      961 ggtatttgtc ctcatcatct tcaagatatg accagtaatg acgaatggca aagtcagcta
     1021 actgacgaac cttttccggc tcgcggcgat agtaaaaatg ttcgaaatga ccaaagcgca
     1081 gatgacttgg tgccacacgc atcagcatcg cgcctggctc caccgtttcc cgatacactg
     1141 gcgaatcgct ggtgacgata cttaacgcgc gggtcgtcgg aatgcccaga taatgcatcg
     1201 cctcactggc gagactttct cgtatcgtcg aacgtaaaac cgcccgtcca tcacccattc
     1261 gcgaataagg cgtcaggcca gcacctttca gatgccagtc cattgtagtg ccatcagcaa
     1321 gcagttgttc gccgagtaaa ataccgcgcc catcacccag ttggcccgcc cagacgccga
     1381 actgatgacc actgtaaacc tgcgccagtg gtgacatacc aggcagtagg gtttcgccgc
     1441 cccagacacc tgcgccattt ttaaacagcg acgatggaat actcagcgtg ttagcgagtt
     1501 cggtattatg ccaaatcagc cgggcattat ttaaaggcgt aggggaaagt gctgtatagg
     1561 ttgctggcaa ttcatcgcgc cagcgggtaa taaaagacag ggtcatagat cctcctgttt
     1621 aatagtgtag acggttactc tcgtcttaaa caccagcaaa caggagggtt atcgctgaac
     1681 caacgtcgtt atctgattta taggaacggc aggccacagg catccctgga gagcatggaa
     1741 atcgaagggg gtaatctgtg ccaatatttc tgtcgtatca atgccccccg cgatgatgca
     1801 attgcaacaa ggggaaattt gcgcctggat cgcacgaata aacggttcga aggagcgatg
     1861 cgtgatttgt tgctgaataa aacttttatc cagcatgacg cgggtgaaaa gaccatcaaa
     1921 aaccgctttc attgtgctat tacctgctcc caaattgcct aacacaaggg gatacatttg
     1981 cgataaggat aaaagatcgc gattatcttt tccttcattg agatgcgggt aattctcatt
     2041 aatcaacagt tcaataaacg gatattttaa tagttcgcct gcgtaattat ccctctctaa
     2101 taataatgta gccacttgtg gcgttaaatt aagccaggca aagagtttat gttgaataaa
     2161 aaaatgttgg catgatttta gtaattccaa ctgctctgaa aataattgcc aatgttgctc
     2221 ctcggtaagt tgcgctataa cccgactcgt cgggatacga accgtaccat cttcactgga
     2281 gaaatgggta attaactcaa cgccgacaag gtcttgctgg ttatctctta tcggcaggaa
     2341 atagcaatca gaatgataaa gattctccaa aaaaatcttc atggtaaccg tccctctcga
     2401 aggatgtttt tcagtatccg gctaaaaggg atgaggccat aagacataac aattacataa
     2461 ggagtaactt tcatttgttc catgttaacc actttttcag gggtcttttt ttagattatc
     2521 ctgattataa acgaataatc catttacgga atttttgtct gcaaaatact actgtattca
     2581 ggagtaaatg gacggcgatg tatcatcgcc gtccattttt cgacatacta aattcgtctc
     2641 gcctgccaga aatttttctg ccaatagaca ttatcaagtg aggaacgcat cactcccttg
     2701 ctggtagagg cgtggataaa ttggttgttg gtatcgtaaa tacctacatg caaaccattt
     2761 tgtccggaac ccgttttgaa aaagaccagg tcaccaggca gcaactcgtc tttatcaatt
     2821 tgcgtgccga tagaggcttg ttctttggtt tctcggggca gctgcaaatc gaaacgatcg
     2881 cgcatcgtta caaccacaaa tcccgaacag tccacaccgc gccgcgtcat gccaccataa
     2941 cgatacggcg tgccatgcca gctttgtagc tggtcgttca aaccggcaat aacggtaatc
     3001 gaatcagaaa gtctggcatt tggcggcggt gctttatggt ggctacaccc ggccagaaac
     3061 agtgctgtga tcaaaataag gcagaaacgc attccgtacg gttcctctgt tttttattct
     3121 tgcattaatt tagcgtcgta attacccgat tttcaagata ctaatgaaat cagatggtcg
     3181 aaatcagcat tctgtgacct tcgatatcca gacggcgaaa attcatccca taggcctgcg
     3241 ccagatttgg cggcgtgagc acctcttctc tgcgtccact ggccagcatt tttccacctt
     3301 ttagcaacca cgcccgatgc gcatgacgca atgtgtggtt gagatcgtga ctgctcatca
     3361 caatcgccag tccttgctga cacagagcgc tcagaatttt gtctaacgca ctttgttgcg
     3421 caacatcaag actgttcatc ggctcatcaa gaagcagcaa ttgacctgcg ggattggctt
     3481 gtggtgtgat ttgcagcacc accgcagcaa gacgtacgcg ttgccattca ccgccggaaa
     3541 gttgattggt gctacgtccg agtttgtcat caagagccag cgcccctgcg acatcattca
     3601 gtagttcggt acgcgtttta tcgtgctgat gcagtgtcag gtagtgccag accggcattg
     3661 caaacggcgg cgtctgctgt tgtgaaaggt aggcgcgatg cagcgcgagt tttgttgcgg
     3721 accatgcttc cagtggttgc cccgcgaact gaatgcttcc cttaccgctg gtcattccgg
     3781 ccattcgtgc aagcaaggta ctcttacccg cgccattcgg ccccaccagg tgcaggatct
     3841 ccccagcccg aacctcgcca gaaagcggcc ccaggcgggt agtttccgca acatcctgta
     3901 actgcatcac aatagacatt attttgccaa cgccagttta atgctttcca tcacaatggg
     3961 atcttcaggc gtcatatccg gggaaaaacg ctggatgacc aacccgtccc tgccaactaa
     4021 aaatttctca aaattccata aaatatcatc agggtacagc ggtgcacgac ctttgctgac
     4081 catacgggca tagaatccgc tctcttccgg cgcgactgcg gtcggcgctg cggcaatcaa
     4141 tttttgatac agcggatggc gtccttcgcc attaacttca atcttactga acatcgggaa
     4201 cgtcaccccc catgtggtgg tacagtaagt tttaatctct tcatcgctgc ccggttcttg
     4261 ttccagaaac tggttgcacg ggaatcccag caccacaaaa cctcgatcgg cccaggcttt
     4321 ctgaatattc tccaactgct catattgcgg cgttaagcca cactttgagg cgacattgac
     4381 aatcaacagc acattaccag cgtacttctc cagcgtggtc acttcaccgt cgatatcttt
     4441 cactacggtc gtcagaatgg aatcttgcat cgtttctcct gggtgtggtc agtaaaaatc
     4501 ttagctttta atcatagacc gtctttttgc ggctaacgtc ttgcttttaa caataaccag
     4561 ataaacaccg gcgcacccaa cgttgcggtg accacgccaa taggcagctc tgcggcagct
     4621 aatgccaggc gcgctacaat atcggccagc agcaatgcgc tcgcccctgc cagcgcgcag
     4681 ccgggaagta atacgcgatg atcggttaaa ccacacaacc gaagaatatg tgggatcacc
     4741 agaccaataa agccgatagc acccgccagc gccacactga cgccaaccat ccagccggtc
     4801 gcggccacca gcacattgcg ccagaaccac aggggtaaac ccagttgccg cgccgagatc
     4861 tcgccaagtg ctaacatatt catcggcctg gactgacaac agatccacaa caacacgggg
     4921 atcaatgcca gcatcagcca gctttgccgc cagtctacgc cgccaaaacc gcccatcatc
     4981 cagtacatca gctgacgtaa atcaacagag gtggaaaagt agatagccca cgtcattagt
     5041 gcgctacaga taatccctaa tgcaacgcca gccagcaata accgactggt cgaaagatga
     5101 cgacgggcga aacgtaagag tattaaagtg atgataagcg cgccagcaat cgcacacagc
     5161 cctagcgccc agttagggag ttgcccttgc ccaagcaata ccgcggcgat aagccccacg
     5221 cctgcgccgt tagagacgcc aagtagtcca ggttctgcca gaggattttc aaacaacgcc
     5281 tgcattacag cgccggatat agccagcgcc gcaccaacca gcaatacagc cagcgtacgt
     5341 ggcaggcgaa tttgccagac gaacagttcg ccacgaggag taaaccagtc acctggcgag
     5401 atccattgtt caccggcgca aaggcttaag agaagcgcca gcagcatcaa aactgacagg
     5461 cataataacc agcgaatatt ttgtcgctgt tgttggcggg caagtgtcag catggtatcc
     5521 gttctgctga agtgtcatgg cgttgatttt acggtgactc ttcgacagtg aaaagaaaaa
     5581 aggccgcaga gcggccataa acacaaacaa aaatcaataa gttagataat tatcaaagac
     5641 ttacagacac acaaaaacac agccaaccac aacaaataac agggatgtgg tcactttgtg
     5701 gatcattcac tcccctcttt ttccttattt cgttgaacgc caattgcaac acacgtacta
     5761 agcaaatctt tacatttctc tacgtcttca gacttacgca atttgatgct ttttacagac
     5821 tgcgggctac tgtccgtagc acgatacatc gttatttcaa tatcatttcg gtctaagagt
     5881 cccataatgg cttttgcaac ctccacaccc ttcttcgcga aatcaaagaa tagagcaagt
     5941 ttatatggat tttcgacgga aaaaacttca attttgctaa aaccaacatc gtgtagagaa
     6001 aaatattgcc caacctcatc tcgaagttct ggatcgcata caatgtctat tctccagtac
     6061 tgacctaaat gcaaatcgct attaactgtt ttttccacaa catcctcctt acccagcaat
     6121 aggattaaat ctcaccgcat cctgcaagta atccggcgca agatgggcat aaatcatcgt
     6181 tgtctgaatc tttgcgtgcc ccagaatttt ctggagcgtc agaatattgc cgccgttcat
     6241 catgaaatga ctggcgaagg tgtggcgcag cgcatgaaca gcctggccgt caggaacatc
     6301 aggtgcgacc gttttgatga catcgcgaac caatgaataa tccagcgtcg gaaacaccag
     6361 tttcccgccc cgttttttga tcttttcaaa caggctttca gaaataggaa cggtacggtt
     6421 tttgctgttc ttcgtttttg aaaaagtgat tcgacaatga agaacacggc gctgctccag
     6481 tgccgctacc tcgccccatc gcgccccggt cgacagaagg atttcgacgg ccagccgttc
     6541 atcgggattt tcagccagtg catccagcaa ctgaacacat tcagacttac tcagatatcc
     6601 catttcacgc tcgttaacct tcattccttt aaggccttga acggggttat cgttaagaaa
     6661 atggccggat gagatgagtg cggtaaacat cgcgcttaac gccccaatct ctcgatttat
     6721 ggtgctgggc tgtatcccct gctctatcct ggacacacgt agctcggtga gcatcgttgt
     6781 attaagttta tgcacgcacg ggtcatccat tgcctcactc aagcgcagca atttaaggcg
     6841 cgtgttatgc cctgacttca ttagctggcc gtggtatttc caccacaagt caataagcac
     6901 ctggctgatt gaaaaagctg gaatcgtggt tcacagatgc caacgcaacc catttaccgg
     6961 aaaccgcatt tttgctctga gcaacggcga caatcagttt ggacaggatt tcacattgta
     7021 cgaagcactt cgcacggttg atcgtctgct tcgcgggcaa agttttatta aacagactga
     7081 tttataacag gtgcgttatg accaaagagc atgcacaagg tgtatttatc cgttttattg
     7141 attttcgcgg tgaactgtta ttgcgcgcat cagctattga tggagttgtc ccatcagaaa
     7201 aaaatgcagc tacttacgtt tatctgaacg gcacgcgcct gaccgtagaa cttccgtacc
     7261 agactgtaca cggaatcatt agcgaagctg aaaaagcacg taagattaat ggcgatgaac
     7321 cctatatcga aattatttgt atggattcag aagctgaaat tcagaaagca gattaaagga
     7381 cgttgcgatg ggcaaagaat ataaaactct cattaacaaa gcacttgagc gtttttattt
     7441 tcgcttaagt gcatcaggtg ctcatgctga acgtgcagcc cgtgactcat tgaccagggc
     7501 aatccggagt ctgtatgacg tggcttttta cgctgatgat ctggatgcac ttaacgaact
     7561 ttccgagctg atctgtgccg cagaatgcgg ggagcatatt gaaccgtata agctggggaa
     7621 tattgcatga gtatatttat ctcatggctt
//
LOCUS       DAZGTR010000175         7583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGTR010000175 DAZGTR010000000
VERSION     DAZGTR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.177"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            46..570
                     /locus_tag="ACHLCY_004479"
     CDS             46..570
                     /locus_tag="ACHLCY_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFP0371582.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            654..1340
                     /gene="lpfB"
                     /locus_tag="ACHLCY_004480"
     CDS             654..1340
                     /gene="lpfB"
                     /locus_tag="ACHLCY_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP0371583.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1364..3895
                     /gene="lpfC"
                     /locus_tag="ACHLCY_004481"
     CDS             1364..3895
                     /gene="lpfC"
                     /locus_tag="ACHLCY_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFP0371584.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTVYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3906..4961
                     /gene="lpfD"
                     /locus_tag="ACHLCY_004482"
     CDS             3906..4961
                     /gene="lpfD"
                     /locus_tag="ACHLCY_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFP0371585.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4967..5491
                     /gene="lpfE"
                     /locus_tag="ACHLCY_004483"
     CDS             4967..5491
                     /gene="lpfE"
                     /locus_tag="ACHLCY_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP0371586.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5744..7435
                     /gene="eptB"
                     /locus_tag="ACHLCY_004484"
     CDS             5744..7435
                     /gene="eptB"
                     /locus_tag="ACHLCY_004484"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP0371587.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
BASE COUNT         1982 a         1735 c         1907 g         1959 t
ORIGIN      
        1 tatgaacaac atcaagcata tttatctata taaatggagt tataaatgaa aaaggttctg
       61 tttgccttat ctgcgcttgc gctgacctct acttccgtat tcgcagctga tgcgggcgac
      121 ggtactgtta aattcaccgg tgagattgtt gacgccgctt gtgttgtgtc tccagacact
      181 caaaaacaag aagttgttct gggtcaggtg aacaagtctg tatttactgc tatgggtgat
      241 aaatccacag caacgccgtt caaaatcaaa ctggaaaact gcgatatctc cactttcaaa
      301 aatgtggaaa tcagctttaa cggcgttggc gatgcagaca acagcaaact ggtttctgta
      361 agcactgaac caggtgccgc aactggcgta ggtatcggta tttatgataa caccaatacc
      421 ctggttgacc tgaatactgg taaatctgct actgtcctga aagaaggtca gaccgttctg
      481 tatttcaccg ctaactatgt ctctaccaaa gatgcagtaa ccacgggtta cggtaacgct
      541 gaagtcgact tcaacctgac ttacaactaa tcgatcctga cggaacgatc atctctcaat
      601 ggcaacagag actgtgttgc catcattcac accaatgact cagggaccga atcatgaaac
      661 gtattagctt tgctgtgctg gcacttttaa tgaccgcaca acagtgtttt gctggtggcg
      721 tagccttaag cacgacgcgt gttatttatg atggcggcaa gaaagaagcg tcgctgaccg
      781 ttgaaaacca taataaaaat gaagagtttt tgatccagtc atggattgat gatgtgaatg
      841 gcaataaaaa aacaccgttt attattacac cgccattatt caaacttgat cctgataaaa
      901 ataacgtctt acgtatcgtc aatattgaag ataccttacc taaagatcgt gaatccgttt
      961 actggattaa tgtaaaggcc attcctgcga aaagtgctga aaatgaaaat aaaaacgtct
     1021 tacagatagc cgttcgcacc cgtttgaagt tgttctatcg tccaaaaggt ttgccgggaa
     1081 gcaacctcga ggccatgaaa cagttacgtt tccagcgtca aaataaccaa attgtggtta
     1141 ataacgactc cgcttataac ctgaccttta atcagttttc cattaatgga caaaagattg
     1201 aacgggcggg aatggttctc gcgaaaggaa agttaaacat taaccttcca gccggtacag
     1261 gcaacgcgca cgaagtgaaa tacagcatta ttaatgattt cggtgttgct ggcgaaatgc
     1321 tgacgaagcc gattaactga tccttttaca aggcgctttt actatgcatt ggatgcattt
     1381 acctctctat cactacagag cgcatttttc cttctctctg ctggcattaa cgattgccag
     1441 ctcgttacct gcgtatggcg gaaaatttaa ccctaaattt ctcgaaaacg ttcagggtat
     1501 cgatcaacac atcgacttgt cggtatacga ctcacctgtc ggccagcaaa tccccggtaa
     1561 ataccgggtt tcggtgtttg taaacgaaga aaaaatggct tcccggacgc tggattttag
     1621 taccgccagc gaagcgaaac gcaaagccag cggtgagtcg cttatgcctt gccttagccg
     1681 ggtgcaactg gaagaaatgg gcgtgcgcgt tgacagcttt ccggcgctga agatgtcacc
     1741 accagaagcc tgcgtagcgt ttgatgaaat cattccccag gccaccagcc gctttgattt
     1801 taatactcag acgctgcatc tttcctttcc acaggcggca atgatgatga ccgctcgcgg
     1861 cacggttgat ccttctcgct gggacgaagg aatacctgcg ctgttgctgg attacagctt
     1921 tagtggcagc aatggacgca atgaaggtac aggttcatct tcagattcga ccagtgacag
     1981 ttattacctg aacctgcgca gcggtttgaa cgttggtccg tggcgtttac gtaataacag
     2041 tatctggaac cgtaccgatg gcaaaaacca atgggataac gttggtacat cgttaaaccg
     2101 ggcaattatt ccacttaaat cacagataac tctgggtgat accgccacgc ctggcgagat
     2161 ttttgatagc gtacaaatgc gtggggcgtt actggcatcc gacgatgaaa tgttgccgga
     2221 cagccagcgt ggctttgctc cggtagtgcg cggcatcgcc aaaagcaacg cagaagtgag
     2281 tatcgaacag aacggctatg tcatttatcg cacttttgtt cagccgggtg catttgagat
     2341 taacgatctc tacgcaacct ccggcagcgg cgacctgacg gtgatcatta aagaagccga
     2401 cggaagtgaa caacgtttta tccagccgtt ttcggcggtg gcgattttcc agcgtgaagg
     2461 gtatctgaaa tacagtctgg ctgccggtga atatcgtgct ggtaattacg acagcgataa
     2521 accacatttc gggcagttta ccgccatgta cggcttgccg tggggtatga ccgtctacgg
     2581 tggtgcattg ttgtcggcag attacaacgc tttagctctg ggcctgggga aaaacttcgg
     2641 taccattggc gcagtatcgg tggatgtcac tcaggcgaaa agtcagttac gcaacaatga
     2701 aaaagaggaa ggccagtctt atcgcttcct ctattcgaaa agttttgagg gtggcactga
     2761 cctgcgtctg ttgggctaca aatactcaac cagcggctac tacaccttcc aggaagcaac
     2821 tgatgttcgc agtgacgccg acagtgatta ccgccgttat cacaagcgca gccagataca
     2881 gggcaatatt acccagcaac tgggagacta cggctcggtt tatttcaata tgacgcagca
     2941 ggattactgg aacgttgacg gtaaggaaaa ctccttaagc gccggttatc acggtcacat
     3001 tggtcgggtg aattacagcg tggcctatac ctggacgaga agtccggaat gggaagaaga
     3061 cgatcgcttg tggtcattca gtgtttccat ccctctgggg ggcgcgtgga gcagttatcg
     3121 catgactacc gaccagaatg gcaagacgtc gcaacaggcc tctgttagcg gcacgttact
     3181 ggaagatcgt aacctgagct ataacgttca gcagggttac accagtaatg gcgtaggcta
     3241 cagtggcagc gtcaacatgg ggtatatggg tggctctggt aacattgacg taggctataa
     3301 ctattctaaa gataaccaac aggttaacta tggcgtgcgc ggcggtgtca tcgttcacag
     3361 tgaaggtata acgctttcgc agcctttagg cgaaagtctg gcgattgttt ctgcaccggg
     3421 cgcgcgtggt ggacatgtgg ttaacagcag cggcgtagaa gttgactgga tgggcaacgc
     3481 cgtggtgcct tatctcacac catatcgtga aaccattgtg gaattgcgta gcgacacgtt
     3541 ggggcaaaac gttgaactac aggaagcgtt ccagaaagtc gtgcctactc gtggtgcagt
     3601 tgtgcgttcc cgctttgata cccgcgtcgg ttaccgtgtg ctgatgagct taaaacaggc
     3661 gaatggcaac gcagtaccgt ttggtgcaac cgcagcgtta attgatgaga gcaaacctgc
     3721 cagcagcatt gtgggtgaag aaggacagct ttatatcagc gggatgccag aagagggcga
     3781 attgcaggtg agctggggga acgagcaggc gcaacgttgc cgtgtgccat tccgcttgcc
     3841 agagaacaag gacaatgcgg ctatcgtgat ggttaatgcg gtatgcgaga aataaggata
     3901 tttagatgaa aattaaaaat ttgttggcag gtatggtgct gttagggacg agcacttttg
     3961 ctggaaatat atgggctgcg gattggggac catgtcagac atcaaatggg gtggcccatg
     4021 agtatagttt tgatttcgta cagactatcc aggtaccttc agaaaataag gctgggaaaa
     4081 tattaactca gccctttgct ttaggtacaa aatattccgc ttattgtgaa tgcccggatc
     4141 ccattccaga taatggagtt gtgacttatt ttaaaggtgt aacgcttctg cctgagcctg
     4201 gaactgtaga tgggtactat aaattcaata attacattga tattctcacc aaaatatatg
     4261 tctacacaca attagatatc ccggtgcctt ttaccgatcg aagcaatggc acggcacaga
     4321 aagaatgtac gccctatact gctaacaatt gggggacagg tggtaaagga agtatcagta
     4381 tttatatttc acatcctttt gttgggcaaa tgattattcc taaaacacga gttgcgtccc
     4441 tctttgggac aaaaaagaaa ggtgtttaca atgatagtca gcccatggcc aatgtcagta
     4501 tttccgggag tattacagta actcaaggct gtgaacttgc cgctggcacc gtactggata
     4561 ttccattcgg agaatatcag gcccatgatt ttaaagggcg tgccggacaa ccaccacaaa
     4621 atgttcagaa ggtacaaaaa gaattaagct ttgactgcaa taatatttca gatggcgtca
     4681 aaatttatct cagtattgat gcaacaccaa atactgccta tcccagcgca attgatttag
     4741 gcaacgccga tgttggtgca gtcattgagg acggtaaagg taatattctt aagccgaatg
     4801 acagtaatag cttattagaa atgaacccag gctcgctgta tgaagatgtc aaacgcaaag
     4861 cgacaaccac aattaccgct tatcccgtta gcactaccgg aaaattaccc gctgccgggg
     4921 attatagcgg tattgctacc atacatgtcg aactggagta acagtcatga agcgaacaat
     4981 acttgcgtta tgcctgctgc ctctttcctt ttcgtcattc gcggcaacca cggatttagg
     5041 gccgaaagga acattgaaat tcacccttaa aatcagccag ggtgcttgcg aactggtgaa
     5101 agacagcgtt gaggttgaca tgggcaccgc ggttctcaaa aagccggtaa gaatcggaac
     5161 ggaaattaat cccactcctt tttccatcgg cctgaaaaac tgtagcgaag tggtcagagc
     5221 ctacgtgact atggacggta cacccaatgc agacgactca aatctttttg cgctggatgc
     5281 gggtggcgct acaggcgttg ggctgaaaat caaaaccgct gctggagtac aacagttccc
     5341 ggaaaataca acgccgacac cggttgagtt tgcaattaac ggtgatggcg cacatcaact
     5401 gaactatatc gccagctacg tgcctattcg cgccgatgca actattggtc gcgcagatgc
     5461 aacggtcgac tttactgtac agtacgaata atttcacatc ttaagggcca gttaactggc
     5521 cctttttact gctttttgta acgctaaagt ctcttttcaa acttgcattt ttgtaaattt
     5581 gtgcttcatg cacactcttt ccccacactt tttcgctttg ctgtggtcta cttattcgcg
     5641 cgtgtagatt ttacttatct gactacctcc gcacttttcc cctgccgggc ctgaaaagcc
     5701 gctaagcagg gtgttatcac ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc
     5761 gattacacag cagaagctga gctttttgct tgcaatctat attggccttt ttatgaatgg
     5821 cgcggttttt tatcgccgct tcggcagcta tgcgcacgat tttaccgtct ggaaaggcgt
     5881 ttctgctgtt gttgaactgg ccgccaccgt actggtgacc ttctttttac tacgtcttct
     5941 atcgctgttt ggccgccgca gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc
     6001 aggtgccagc tattacatga ccttccttaa tgtggtcatt ggttatggca tcatcgcttc
     6061 cgtcatgacc accgatatcg acctgtcaaa agaagttgtt ggtctgaact ttattctctg
     6121 gttaatcgcc gttagtgcat tgcctcttat ccttatctgg aataaccgct gtcgctacac
     6181 cttgctccga caactgcgaa ccccagggca gcgtattcgc agcctggcgg tcgtcgtact
     6241 ggcgggtatt atggtttggg caccgattcg tttgctggat atccagcaga agaaagtgga
     6301 gagggcgacc ggcgttgatt tgccgagtta tggcggtgtc gtagcgaact cttatctgcc
     6361 atcaaactgg ctttctgcgt tggggctgta tgcctgggcg cgggtcgatg aatcttccga
     6421 taataattca ttgcttaatc cggcgaagaa attcacttat caggcaccgc aaaacgttga
     6481 tgacacttat gtcgtgttta tcatcggtga aaccacgcgt tgggaccata tgggcatttt
     6541 cggctatgag cgtaatacca cgccgaaact ggcccaggag aaaaatctgg cggcgttccg
     6601 tggttactcc tgtgataccg caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg
     6661 gggcgcggaa gataatccgc agcgcacatt aaaagaacag aacattttcg cggttctgaa
     6721 gcagttagga ttcagttctg acctttacgc tatgcagagc gaaatgtggt tctacagcaa
     6781 cacgatggcg gacaacattg cttatcgtga gcagattggt gcggagccac gtaatcgtgg
     6841 caagccggta gatgatatgt tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc
     6901 ggatggtaag catctgatca ttctgcatac caaaggttcg cattttaact acacccagcg
     6961 ttatccgcgt agcttcgcgc agtggaagcc ggaatgtatt ggtgttgata gcggctgtac
     7021 caaagcgcag atgatcaact cctatgacaa ctcggtgacc tatgtggatc actttatctc
     7081 cagcgtgatt gatcaggttc gcgataagaa agcgattgtg ttctacgcag ctgaccacgg
     7141 tgagtcaatt aatgaacgcg agcacctgca cggcacgccg cgtgaactgg caccgccgga
     7201 gcagttccgc gtaccgatga tggtctggat gtcagataaa tatctggaaa atccggccaa
     7261 tgctcaggca tttgcgcagc tgaaaaaaga agccgacatg aaagtgccac gccgtcatgt
     7321 agagctgtac gacaccatca tgggttgtct tggctatact tcaccggatg gtggaattaa
     7381 cgaaaacaac aactggtgtc acatcccgca gacaaaagtg gcagcggcta actaagcggc
     7441 ctgctgactt tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat
     7501 ctgcgcagta agatgcgccc cgcattcggt gattggcgca gcctggtagc gcacttcgtt
     7561 cgggacgaag gggtcggagg ttc
//
LOCUS       DAZGTR010000176         7576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGTR010000176 DAZGTR010000000
VERSION     DAZGTR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.178"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..4793)
                     /locus_tag="ACHLCY_004485"
     CDS             complement(<1..4793)
                     /locus_tag="ACHLCY_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease fold toxin"
                     /protein_id="HFP0371588.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEIPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTESNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRSEIKKDVDFPEAGEDTALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKQLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVVPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDAQGRCVNVTGAEGYYNATLDYGDGCTTVTDGKGTHRYYYDPD
                     GNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAALGQLSRYTAADGAEWL
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTAQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQSVRLQWQQHHKLSG
                     LERAETGAEGFRYDRHGNLLAYTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEVILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEETTWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDGYRPETERFRWDSR
                     DQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNHAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGEWDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     TGMYLVSDPLGLQGGEQTYRYVPNPLGYIDPLGLAKTSVPAEKISLSDKARDLFRQGK
                     VREALDVHYEDLVRRKLGGISQEIAGREYDVVTDKIIAQVKRTYSSIDNPKNFLSKST
                     RTQIKKTIELAEEQGKEAQFWFKYGVSPKVREYIESKGGKVILGMG"
     gene            complement(4813..5274)
                     /locus_tag="ACHLCY_004486"
     CDS             complement(4813..5274)
                     /locus_tag="ACHLCY_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP0371589.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITQAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5302..7203)
                     /locus_tag="ACHLCY_004487"
     CDS             complement(5302..7203)
                     /locus_tag="ACHLCY_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0371590.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDELQRQVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVRF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1435 a         2285 c         2014 g         1842 t
ORIGIN      
        1 cccataccca atataacttt cccacctttg ctttctatat attctcttac tttagggctt
       61 actccatatt taaaccaaaa ctgagcctct ttcccctgtt cttcagccag ctcaattgtc
      121 ttcttaattt gtgtccgagt agacttactt aagaagtttt ttggattatc tatagagcta
      181 taagtcctct tgacctgcgc tatgatcttg tcagtaacaa catcatattc acgcccggca
      241 atttcttgag atataccacc aagctttctt cgtacaagat cctcataatg aacatctaat
      301 gcttctctta cttttccttg tctaaaaaga tctcttgctt tatccgaaag gcttattttt
      361 tctgcgggaa cagaagtttt tgccagcccc agcggatcaa tataccccag tggattaggc
      421 acataccggt aagtctgctc acccccctgc aaccccagcg gatcactcac caggtacatt
      481 ccggtttccg gctcgtagta ccggaaccgg ttatagcaca gccccgattc cgcatcctgc
      541 cactgtcccg catacagcag gccggggtcg gcttccgggt cccactcccc gcgcgggtcc
      601 gggtagccgg tgtctgcggg caggctgagt gccagccccc acaggctggt ctgtcccggc
      661 cgccagacgg ttttaccttc actgttaaag agcatcagcg ggcggcccgt caggtcgctc
      721 accgcatgat tcgtccgcgt cacccactgc ggggccaccg acggatgcgc ctgccgtacc
      781 cggctgaact gctggcttat cagctcaaag ccgttaaaca ccaggtgccg tacgctgtac
      841 agtttatcat cgcggtattc ccggatttcc gcaatactgt cgccgtccca caggtacgtg
      901 aagcggattt tcttccggtc gcagcgtttc tctgttcgtc tgccgctggc gtcgtggcgg
      961 tattcccact gctcaccctg tgcgctgcaa tacccggtca gctggtcccg gctgtcccac
     1021 cggaaccgct ctgtttccgg gcggtagccg tcacggtgtc tggtgcggct gaccatccgg
     1081 cctgcggcgt catagtcata ctgcgtgttt ccggcctgtt tcagccggtg gcctccggca
     1141 tactggtcag tctcttcact tatccggtgc ctgcccgcag actgcgcctt caggtagcca
     1201 caactgtcat agccatagga ctcatcgcct tcgccatagc ccagcccggt gcctgaggct
     1261 gtcaccgccg tgacctggcc gttccggtcc agccggtact cccgccgggt ttcccgtccg
     1321 taatcttccc ggctggtgac agccgacaca ttgcccgcgc tgtcgtaacg gtactcccgt
     1381 accagtgagg ggagcagccc gccccatgtg gtttcctccg gaaaaaatcc ggcgcgctga
     1441 ctgacaggct ggccggctgc attccagccc tgttcacagg caaaaccccg gttactgtgc
     1501 cggagcactt cacgtcccgc ctcatcacgg ctgaacgtca gcgtctcatc ccccggcaga
     1561 atgacctctg tcagttctcc ggcggcgttg tagcggtacg tggttttcag cagggattca
     1621 tcggggtgtc tgtcgcgttc atcttccggt ggcaggatac agcggataac ggtgtgttca
     1681 tcggggtagt ggtattcaat ggcccgtcgg ccctgagtgg tgacattcac ccgcccgcgt
     1741 tcgtcatagc cggtgcgctg tgatgtgccg tccgggctga agatacccgt cagcctgccg
     1801 gatttgtcat acgcatactg cgttttgcct tccggcgtga tgacttctgc caggcggcct
     1861 gcggcgtccc ggctgtagag gatggtttcc tccaggccgt tgaccctggc ggtacacagg
     1921 ccatcagcgt catagcggta acgccagacc acgccgcccc agtcacgctc ttccgtcacc
     1981 cggccacagt tgtccagaat ataacggtat gactcgccct gcgggttaat gacttctgtg
     2041 agctgcagcg tgtctttatc gtagcggtac tgccagcggt ggccttcacc gtccgttcgc
     2101 gccaccggca aatcgaacgg gccgtattcc attgtccaga caacgccgtt accgtccgtg
     2161 tacgccagca ggttgccgtg gcggtcatag cggaatcctt ctgcgccggt ttctgcccgc
     2221 tcaaggccgc tcagtttatg gtgctgctgc cactgtaacc gcactgactg accgccgggg
     2281 ccgcgcatct tcaccggcag gtcgtgaccg ctgtattcgg tgtggtgttc acggcccatc
     2341 acgtcggtga gtgtttccgg ccggttgtgg tggtcgtact ctatgcccag acgccgttca
     2401 tcctggcgga atatccccga cagcagcccc tgagcggtgt atgccagccg ggtctcttcg
     2461 ccctgtggcg acaccaggct taccggcagg ccgcgctcat cacactgctg cgtccatgtc
     2521 tgcccggcag ggtcggtgat gttgctgagc aggccgcgct catcatagcg gtacagccac
     2581 tccgcgccgt ctgccgccgt gtaacggctt aactgaccga gtgcggcgtt gtattcaaac
     2641 ttctccaccc gcccggcagg ggagtggcgg gccagcaggt gatggaattc atcccattca
     2701 tacgtggtgg tactgccgtc cggcgcttct tcccgcagaa tattgccgtc aggatcatag
     2761 taataacggt gagtgccctt gccgtccgtc acggtggtgc agccgtcacc atagtccagc
     2821 gtggcgttgt aatagccctc cgccccggtg acattcacgc accggccctg tgcatcgtag
     2881 gtgaaacggc tccacgtctg gtcgttatcg gaccagcgga tgatccggtc cgcagcgtcg
     2941 tactcataaa acaggtggta gtccagccgc gcatccgctt ctgtcagccg cccccgcgca
     3001 tcctgttcgt aggtcgccag cgtctgtatg ccgttatccg tgcggtgaat ggctttcagg
     3061 tagccgctgg cgtggtacgt cagtttaagc cggatcccgt cggtgtgact gacacccgtg
     3121 atccggtact ggctgtcgcg ctcaaacgtg atgcggttgc tggcggtgtc gctcatcccg
     3181 cccagcatat acatccggtc gtcggggctg tgtgccaggc ggtcatcgct ggtatcgtca
     3241 gtcaccgggt ggcgcagacg cattcccgga aaggcgtcat aaaagaagcg gctgctcagt
     3301 gcatccctgt cgaacagctc caggtaatcg tcgcggcggc gcagaatgta ctgggcgtga
     3361 tacgggttaa ccgccgtatc actgtcaccg tcgaaggcga agttcagcgt ggctccctgg
     3421 ctgataatat ctacccgatt tccctgttca cgcacccagg cgtattcgct ccaggagtcg
     3481 ctccagccca cgccggtcag gccatgcagc ggcagtacag aactgtggaa acgggtgaag
     3541 gagaggggga gggtctggcc cagggtgaag tcggtacgtt cgtcgcagta tgcgccggtt
     3601 accggaacga caggatctgt tacccatctg atgactttat tggcaatact ttttaacgac
     3661 ttacctgttt tccccagcaa cgcgccgagc tttgcgccca gctgcttccc gcttttcccc
     3721 agcaggtttc ccagtttgcc gagtctcgcc agattcttca gttttcccag cagtcccgca
     3781 ggcggcagaa gatagctggc tatcgttatt ccccccagga tcattctctg ccacggcggg
     3841 aactccggct ggatatccag ataggtcacc tgttcaccgc caataaagac gtccggcgaa
     3901 cctcccacga tcgccgcact gcactccagt ttgtcgccct tgcgcgcggc aggctgattg
     3961 ttgataaaga cgttgctact gccctgggct atcctcggtg gtttgttctc cttgtcgcag
     4021 agcgccgtgt cctcaccggc ttccgggaaa tccacatctt ttttgatttc gctacgcccg
     4081 cccaccatgg tggcaaactc cattaatacc ggaccggcgg gaaacagaag cgatgcgccg
     4141 gcgctgacgc gatccataaa accggcattg ctttccgtca gggtgataac agcgttcccc
     4201 caggccttcg ccagtggtac tgctttttcc gctgccgatt caagcaggtc agtggcaata
     4261 tcaccccatg agggttcccc ctgtgggatt tcaggtattg ctccaggtgg gggaggaggg
     4321 ggaagggtga cggcggccct tgcagcgggc tttccgttaa tgtttacatt ttcagaaccg
     4381 gtattgatgg cgccgtccgt gtcacaaaag ctgtttatcc atgattcaag ctggttctgt
     4441 ttctcctcca cataatcact cgccttatag ctggcataca tcagcgcagg caccagaacc
     4501 agcgcataag ggccggtggc gcaggcggcc ataatcaggc aaccttctgc gatggtcact
     4561 atcgccccca gcacggcccc tgccagtgcc cccagaaaac ttttatgctg aatttcatcg
     4621 cccagcctgg cggcaggtgg gccggacttc tttgctgcca gcatctgtgc aatggctttg
     4681 tcgttggctg cccgtttcat ctggctggcg accatcgcct gctgtgacag cattcgcatt
     4741 gccagcgtac cgcctgcggt cgctccctgt ggcccgccag gtccttcact catgtgttat
     4801 ccctcgttta tgtcagcgcg gctggtcctt ttggatgtca ttctccggtg gcaacggggt
     4861 gaagctcttt atcacctcca gcagcatcgt cttttgactg ttcttcaggg ataaggaggt
     4921 gctgaaagtg aaggtcagca gcgtatctcc acgccgctgg aatacggcta tctggctaat
     4981 ccgcccctga ttcccctcat cttccggacc gttccaggaa taatccagca gccaggccgg
     5041 ggcctctgcc tgagtgattt cccggcaggc atgttcggtg tacccatcaa gtttgccctg
     5101 gtacagcgcc agcgtttcct ggtacagctc tggtgtcagg gttccctgtg gaacggcttc
     5161 gcgggtaatg accaggttga tgccactgtt atctgtggac accaggacct gcattgactg
     5221 atcctgccat tgagacggca gttgaatgtg gccttcatta aaagtgaact gcataaaata
     5281 ctccctgata atctgagtga atcagttgat gaaaacagaa ccgcccacga tggtgatgcc
     5341 atccttcgac agcctgatta ccgtcgattc cccgacctgt agctgaatgc catccggcgt
     5401 cagcgcaatt ttgctttcct ccacagccag caaaatttcc ttaatcgccg tataagcctg
     5461 tataccgtcg ccttcgccac cattgtttac agcgtcaaca tgcaccgaac cggcaatcgt
     5521 gcctctgtcc tgaaagacat cctcagataa agaccctttc agtactccgg tgatcttgtg
     5581 gcctttttcc accgtaagaa ggcggttacc ttcctggatc gtgaccgtct ggtcattttt
     5641 tacggtttcg cggtgatcgt gttttacggt ggtggtacgg tcgttgagca cttcggtatc
     5701 catgttcttc tgcgcgtgga tgtagacctg ttctctccct gtcgcatcat caaacttcag
     5761 ctcattaaat ccgctgccca tgtacgtttt ggagcggatg gtcatctgcg tctttgttcc
     5821 cggcaggctg ccgggggtgc ggttttcctg gtggtaggtg cgccccataa tgatcggctg
     5881 gtccggatcg ccgttgagga agtctacaat cacctcctga cccacacgcg gtatcgctag
     5941 gtggccaaaa ccggtgcctg cccacgcctg tgccacacgg atccagcacg aactgtcttg
     6001 gtctgccggg ttataacggt cccagttgaa cctcacccgc acgcgaccat gttcgtcgca
     6061 gaagatttcc tcgcctgccg gtcctgtcac gacagcgctc tgcgggccgt cgaccagcgg
     6121 tttcagcaac ggctgtggtc gccatgttct gtctgccggg atcaccgcaa aatggttctc
     6181 cagcgccgtc cccgctccct gccgtcctgg caccgcctgt ggctgttcgc cgtgcagttc
     6241 acttgccacc acctgccatt cccggttcag gttcgcctgc ggatgccccg tcagcacaat
     6301 tcgccgtccc ggccatatct ccggtgagcg gctcattccc cgcgcggttt ctgcgttgtt
     6361 tcgccagccg tccatctgcc agcgggcaaa gttctgcccg tgggcgctct tgaaacgtcc
     6421 ggggtagtca tacacttcat actgcgtgcg ctggtagtcc tggtgctggc cttcctgttc
     6481 aaaacgtccg gcccagccgg ggcgtttaaa ggtgtagtct ttggtcacca cggaagaagg
     6541 gcggatttgt gcgctgtagc ggaactggct gatgcagagg gtgctcacct cggtacgggt
     6601 gttcgggttc catgggattt caaaagattc gggcagatgg cggactgtgt cgcacagcac
     6661 caggctctgg tcggtacttt tgtaagcatg ctcctcataa aagaagatgc cttcctccgc
     6721 cgccatccgg cacagaaaat cgtaatcagt ctcaccgtac tggacacaaa actcacggga
     6781 aggatgcggc tcgctgaaca gcggactcca ttcggtcacc ccgttttcct gcaacatcgt
     6841 gccgaggatg cttttgatgt cctcgttctg gaagatacgg aagttctggc gcagaccggc
     6901 acgccacagc ggcgggtgca ccttcatgct gtacagcatc tggtttttgt cgttctcccc
     6961 cagttcaaac caggtcacca cgcctttcac ctggcgctgt aattcatcgc cctgccatat
     7021 cgtcaggtag gccattttgt ccagcacctg cgcaaattca agggagagaa actgctggct
     7081 gaccagggag agatcgaggg aaaagagcga agagagtgac tggttcagat gaaaggaaac
     7141 caccgcaaaa gcgtccggcg gcaggccgtc cacttccagt gtgaaacgta atccggttga
     7201 catagcaacc tccggtaaaa tgaataatga tccgtaccgt acaactgcgg ataatgtgat
     7261 aagcgggacg acttcagacg ttattaggga aggaacagaa aatgctatgg ttatgtgaga
     7321 attttgttta aatattactt tacagaaacg ggtgtctgtt cagtcaaaaa aataatcaca
     7381 atctcaacct cttgtttctt ggcgttattc aggacaggta cgcatatcac aacgagtaac
     7441 aaaaacgcaa agttaaacca atattcataa tatattttta ttcacagcaa taaataatcc
     7501 aattaacatt attaaaatga atgattgcat aagaaatata tgctaccgct ccaccctgat
     7561 aacatgatct gcacat
//
LOCUS       DAZGTR010000177         7526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGTR010000177 DAZGTR010000000
VERSION     DAZGTR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.179"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..853
                     /gene="gatY"
                     /locus_tag="ACHLCY_004488"
     CDS             <1..853
                     /gene="gatY"
                     /locus_tag="ACHLCY_004488"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP0371591.1"
                     /translation="YVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPV
                     IIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMI
                     DASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQAR
                     EFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQ
                     QTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIAD
                     CGCEGRA"
     gene            882..2144
                     /gene="gatZ"
                     /locus_tag="ACHLCY_004489"
     CDS             882..2144
                     /gene="gatZ"
                     /locus_tag="ACHLCY_004489"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP0371592.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2154..2606
                     /gene="gatA"
                     /locus_tag="ACHLCY_004490"
     CDS             2154..2606
                     /gene="gatA"
                     /locus_tag="ACHLCY_004490"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP0371593.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2637..2921
                     /gene="gatB"
                     /locus_tag="ACHLCY_004491"
     CDS             2637..2921
                     /gene="gatB"
                     /locus_tag="ACHLCY_004491"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP0371594.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2925..4280
                     /locus_tag="ACHLCY_004492"
     CDS             2925..4280
                     /locus_tag="ACHLCY_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP0371595.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4328..5368
                     /gene="gatD"
                     /locus_tag="ACHLCY_004493"
     CDS             4328..5368
                     /gene="gatD"
                     /locus_tag="ACHLCY_004493"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0371596.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5474..6247
                     /locus_tag="ACHLCY_004494"
     CDS             5474..6247
                     /locus_tag="ACHLCY_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0371597.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6329..7228)
                     /gene="yegS"
                     /locus_tag="ACHLCY_004495"
     CDS             complement(6329..7228)
                     /gene="yegS"
                     /locus_tag="ACHLCY_004495"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP0371598.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1892 a         1692 c         1954 g         1988 t
ORIGIN      
        1 gtacgtggta tcgacaaagc agatgctgaa caacgcacag cgcggcggtt atgcggttcc
       61 ggcattcaat attcacaatc tcgaaacgat gcaagtggtg gtggaaaccg ctgccaacct
      121 gcatgcgccg gtcatcatcg ccggaacgcc tggcacattt actcatgctg gtacagaaaa
      181 tctgttggcg ctggtcagcg cgatggcgaa gcaatatcac catccactgg caattcatct
      241 cgaccatcac acgaaatttg acgatatcgc tcagaaggtt cgttctggcg tgcgctcagt
      301 catgattgac gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca aagaggtggt
      361 ggatttttgc catcgctttg atgtcagcgt cgaagcggag ctggggcaac ttggcggcca
      421 ggaagatgat gtgcaagtca atgaagccga tgctttttat accaaccccg ctcaggcgcg
      481 tgaatttgcc gaggcaaccg gaattgattc cctggcggtc gccatcggca cggctcatgg
      541 gatgtatgcc agcgcaccgg cgcttgattt ttctcgactg gagaacattc gccagtgggt
      601 gaacttaccg ctggtgctgc atggcgcgtc agggttatcg actaaggata ttcagcaaac
      661 catcaaactg gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg ccttctcgca
      721 ggcgttaaaa aattacctga ccgagcaccc tgaagcgacc gatccccggg attatttgca
      781 gtcggctaaa tccgcaatgc gcgatgtggt gagcaaagtg attgccgatt gtggctgcga
      841 gggcagggca taacgcacct gccatttaat aaggaaaaaa catgaaaacg ttaattgccc
      901 ggcataaagc tggtgaacat atcggcatat gttcagtctg ttctgcccat ccgttggtta
      961 tcgaagcggc gctggcattt gatcgcaaca gcacgcgcaa agtgctgatt gaagcaacgt
     1021 caaaccaggt caatcaattt ggcggttata ccggaatgac accggcagac tttcgcgaat
     1081 ttgtttttac gattgccgat aaagttgggt ttgcacgcga acgcattatt ctcggcggcg
     1141 atcatctggg gccaaactgc tggcagcaag aaaatgcgga tgcggcgatg gaaaaatccg
     1201 tcgagctggt aaaggaatat gttcgtgccg gtttcagtaa aattcatctt gatgcgtcaa
     1261 tgtcctgcgc gggggatccc ataccgttag caccagaaac ggttgcggaa cgagctgctg
     1321 tgctttgctt tgctgcggaa agtgtggcga cagattgcca gcgtgagcaa ctgagctatg
     1381 tcattggcac cgaagttccg gttccgggcg gtgaggccag cgccattcag tcagtacaca
     1441 tcacccatgt tgaagatgcc gccaatactt tacgtacgca tcaaaaggcc tttattgccc
     1501 gtgggctgac agaggcgtta acacgcgtga ttgccatcgt ggtgcagccg ggcgtggaat
     1561 ttgatcacag caatattatc cattatcagc cgcaggaagc gcagccgctg gcgcaatgga
     1621 tagaaagcac ccgaatggtt tatgaagcac attctaccga ttaccagacc cggacggctt
     1681 attgggaatt agtccgcgat cactttgcaa tattgaaagt cggtcccgca ttaacctttg
     1741 ctttacgcga ggcgatattt gcactggcac aaattgagca ggaacttatc gcccctgaaa
     1801 atcgcagcgg ttgcctggcg gtaattgaag aagtgatgct cgacgaaccg caatactgga
     1861 aaaaatatta tcgtacgggt tttaacgatt cattactgga tattcgttac agcctgtcgg
     1921 atcgtattcg ttattactgg ccgcatagtc ggattaaaaa tagcgtcgaa acgatgatgg
     1981 tgaatcttga aggcgtggac atcccactgg gcatgattag tcagtatctt cccaaacaat
     2041 ttgaacgcat tcagtccggg gaattatcag caataccgca tcagctgatt atggataaaa
     2101 tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg tatatgacta
     2161 acctgtttgt tcgtagcgga atttcttttg tcgatcgtag cgaagtttta acccatatcg
     2221 gtaatgagat gctcgcgaaa ggtgtggttt atgatacctg gccacaggca ttaattgcca
     2281 gagaagcaga attccctacc gggataatgc ttgagcagca cgccattgct ataccgcatt
     2341 gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt aaggccaaca aataaagttc
     2401 attttcagca agcggatgat gataacgacg tggaggtgtc gttggttatt gcgttgattg
     2461 tggaaaatcc gcagcagcaa ttgaaacttt tacgctgttt atttggcaag ttacaacagc
     2521 ccgatatcgt cgagacacta attactcttc ctgaaactca gttaaaggaa tacttcacaa
     2581 agtatgtttt agattcagac gaataaatcc ctctgtaaca ataaaaagga ctatttatga
     2641 aacgcaagat tattgtcgct tgcggaggcg cggttgcgac ctctacgatg gcggcggaag
     2701 aaattaaaga gttgtgtcag aatcataata ttcctgttga attaatccag tgtcgggtta
     2761 atgaaataga aacctatatg gatggtgtgc atttgatatg caccactgcc aaagtggatc
     2821 gtagttttgg cgatattccg ttagttcacg gcatgccttt tatttctggt gtcggtatcg
     2881 aagcattaca aaataaaatt ctgactatct tacaggggtg acctatgttt tcagaagtca
     2941 tgcgttatat tctcgacctc ggccctacgg tgatgcttcc gattgtcatc attatttttt
     3001 ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc gggtctgcat atcgggattg
     3061 gctttgttgg cattggcctt gtgattggct taatgctgga ttccattggt ccggcggcga
     3121 aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc tggccgggct
     3181 cttcaccaat gacctgggcg tcgcaaattg cgctggtggc gattccgatt gcgatattgg
     3241 ttaacgtggc gatgttactg acccgtatga cgcgggtggt aaatgttgat atctggaata
     3301 tctggcatat gaccttcacc ggcgcgttgc tgcacctggc aaccggttca tggatgatag
     3361 ggatggcagg tgtggtaatt cacgcggcgt ttgtttataa gctcggcgac tggtttgccc
     3421 gcgatacccg aaatttcttt gagctggaag gtattgctat tccgcacggt acgtcggcgt
     3481 atatggggcc gattgcggtg ctggtcgatg ctatcatcga gaaaatccca ggcgttaacc
     3541 gaattaaatt tagcgccgac gatattcagc gcaaatttgg tccatttggc gagcctgtca
     3601 ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc gggttacgat gtcaaaggtg
     3661 tattgcagct ggcggtaaaa acggcggcag tgatgctgct aatgccacgg gtgattaaac
     3721 ccatcatgga tggtttaacg cccatcgcta agcaggcccg tagtcgttta caggcgaagt
     3781 tcggcggtca ggagttcctg attggcctgg atcccgcatt actgctgggg catacggcgg
     3841 tggtatcggc aagcctgatt tttatcccgc tcaccatttt aattgctgtt tgtgttccgg
     3901 gtaatcaggt gctgccgttt ggcgatcttg ccaccatcgg cttctttgtg gcgatggcag
     3961 tcgccgtgca tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc atcatgagca
     4021 tcactctgtg gatcgcgacg caaactattg gtttgcacac ccaactggcg gctaatgctg
     4081 gggcgttaaa agccgggggt atggtggctt caatggatca gggcggttct cccattacct
     4141 ggttactgat tcaggttttc tccccgcaaa atattcccgg attcattatt atcggcgcaa
     4201 tttatcttac cggtattttc atgacctggc gtagagcgcg tggctttatt aaacaagaga
     4261 aagtcgttct cgcagaataa tttttacctg agggggaggt tatccccctt aacaatcagg
     4321 agtttttatg aaatcagtgg tgaatgatac tgatggtatc gtgcgcgttg cagaaagcgt
     4381 cattcctgaa attaaacatc aggatgaggt gcgggtaaaa attgccagct cgggcttatg
     4441 tggttccgat ttacccagga tatttaaaaa tggtgcacat tattatccaa taacgttagg
     4501 ccatgaattt agcggctata ttgatgcggt gggatccggt gttgatgatt tacatcctgg
     4561 cgatgcggtt gcctgtgtgc cgttattacc ctgttttact tgtccagagt gtttgaaagg
     4621 gttttattcc cagtgcgcaa aatatgattt tattggctca cggcgtgatg gtggatttgc
     4681 cgaatatatt gtcgttaagc ggaaaaatgt ctttgctcta cctactgata tgcctattga
     4741 ggatggggct tttattgaac cgattaccgt tggcctgcat gcttttcatt tagcgcaagg
     4801 ttgtgagaat aaaaacgtta ttattattgg tgccggaacc attggcctgc tggccattca
     4861 gtgcgctgtc gcgctgggag caaagagtgt gacggcgatc gacattagtt cagaaaaact
     4921 ggcactggca aaatctttcg gtgcgatgca aacatttaac agtagcgaaa tgagcgcgcc
     4981 gcaaatgcag agcgttttac gcgaactgcg ctttaatcag cttatcctcg agacggctgg
     5041 cgtaccgcaa actgtcgaac tggcggtaga gattgccggt cctcatgccc aactggcgct
     5101 ggtgggcacg ttgcatcagg atctgcattt aacatcggca acgtttggca aaatattgcg
     5161 taaagagctg acggttatcg gcagttggat gaactactcc agcccttggc cggggcagga
     5221 gtgggaaacg gcgagccggt tgctgacaga acgtaagtta agcctggagc cattaatcgc
     5281 tcaccgtgga agctttgaaa gcttcgccca ggcggtgcgt gacatcgctc gtaatgctat
     5341 gccgggcaaa gtgttgctca ttccctgaaa ccgcgggcca gcgtgatgct ggcccggtat
     5401 tgtgcaaaac agatcattca ccaatggtcc cccttcgttt acactagcca caattgaata
     5461 tgggtaaatg acgatgaatt cattcgagcg aaggaataag atcatccaat tagtgaatga
     5521 acagggaacc gtgcttgttc aggatctggc gggagtattt gctgcctcgg aagcgacaat
     5581 ccgtgccgat ttgcgctttc tcgaacaaaa aggcgtggtt acgcgctttc atggcggtgc
     5641 ggcgaaaata atgtccggta atagtgaaac cgagacccag gaagtcgggt ttaaagagcg
     5701 atttcagctc gccagcgcgc cgaaaaacag aatagcgcag gcggcagtca aaatgatcca
     5761 tgaagggatg accgttattc tcgacagcgg aagtaccaca atgcttatcg ccgaaggatt
     5821 aatgaccgcc aaaaatatca cggtgattac caacagtctc ccggctgcgt ttgccctttc
     5881 cgaaaataaa gacattactc tggtcgtctg cggtggcacg gtccgccata aaacgcgatc
     5941 tatgcatgga tctattgctg aacgttccct gcaggatatt aatgccgatt taatgtttgt
     6001 cggtgctgat ggtattgatg cggttaatgg tattacgacc tttaatgaag gttattcgat
     6061 tagcggtgcg atggtcaccg ccgctaataa agtgattgcc gttctcgatt catcgaaatt
     6121 taaccgtcgc ggttttaatc aggtactgcc aatcgaaaaa attgacatta ttattaccga
     6181 tgatgctgta tcagaggtgg ataaactggc attgcagaag acacgggtaa aattaattac
     6241 ggtgtagtat tgttctgtat gccggataag gtatttacac cgcatccggc atttcggatt
     6301 ctccctggtt caagagagaa tagtttgatt aacgcaataa tggacaatct ggtggtaatc
     6361 gacaacgcaa cgccgccgga agtatttcaa tatgaaagtt ttgtccgctc aacggttcgc
     6421 catcaagatt aaaagtgatt tcgtgtgggg cttgtatatc aaaccacgac gaagcgcctt
     6481 cgataatatt cgggttatct tcgtcagatt ttaaggttga tacgagagcc ggaagaattt
     6541 catcgccggt aaaaatgcgc agttgcagca agccatcgtt aattaacgcg ttcggacaca
     6601 attgctgacc gccaccggcc tgacgcccgt taccaatacc aatgaccagg gcgtcacctt
     6661 gccagtgaaa gttttcaccg cggatttcac aacggtccgg ttgcagagta tccatgcgca
     6721 ttaagccatg aatgatgtaa gagacgccac ccagcgcggc ttttaatttt tccggcgttt
     6781 ctgtggtaat acgcgtccca aatccgcctg tcgccatatt aataaaacag gtttgtttgt
     6841 tgacctgcgc catatctatc gcaatggcgt taccggcaat tgccagtttc agcgccttat
     6901 ccagtgcctc aggaatccct acactggtgg caaaatcatt ggcggttcct aatggcaaaa
     6961 ttcccagcgc gggtatgtca tccccctcac actgaatcaa cgccgtagaa acttcattaa
     7021 tggtgccatc gccaccaccg gcaatcaccg ttgcgacgcc caacttccgg gcctcctcta
     7081 catatcgtgc ggcatcgcct ttctcccagg tgacccgcac atggatcgtc attccttcct
     7141 cacgcaacag cataattgct tcgcgcaagg gtagattgtc agtacttttg ccattaagaa
     7201 tcagtaagct ggcgggaaat tctgccatga tcgtatgtgc ctttatgatt ggtctgtaga
     7261 tagtgtagag cagaaaacaa aaggtggagt cagaacagga tgaaagtcgg aggataaaaa
     7321 gtcagttcaa gtatggagga ttcagtggtt accaccagga gcttgcaatg gggacagcga
     7381 atactaacgg ctgccaccga taaatttcaa aaaagagcat atgcctaata ttcaaccaaa
     7441 cagtggcatc ttaaatatac tattccaaat atattaaagc ccccatggag ttaccctgaa
     7501 gggcctcaat gtccgtaatt cctact
//
LOCUS       DAZGTR010000178         7450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGTR010000178 DAZGTR010000000
VERSION     DAZGTR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.180"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>7450
                     /locus_tag="ACHLCY_004496"
     CDS             <1..>7450
                     /locus_tag="ACHLCY_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFP0371599.1"
                     /translation="NRWKDNSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQS
                     PVKTGARILNEFSRMGLPGVATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKA
                     TGKAVSDGAKATDEWLTGKMSPGAVRALNTPMTEGYNDSAVWVAKGVNLIGALVPDMV
                     AGGVARKVGDVTLRKMLTAGLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITH
                     STVSAQGQSAMAAADAVLNADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKE
                     RVADEVRAQLATDPELLAVNAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAIN
                     AAQGGYSRYQENTALRETAGMGVSPWEGVADATIEGAAFGAAMGAPFGAVAGYRGRRQ
                     AAEETAMRDAETVQQDDAAPQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDA
                     SAAHRREAASQLLNELDEQTKRQAVMNELKAKPRSELLEEYRRLSQKEGRTETEEQQF
                     QAIREVIRPQQEVTPEAQSQPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAER
                     IEKVRPDNRYFTDEKSAMGSDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVAT
                     ETINTLADGERIADADPMEQPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQ
                     MAEGDAGPTAQELTERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEV
                     KGQSFQKGQAPAPENAAGRQGETLEGDMVRRGLPSPDAQNATAPVREGLPAPDIARNV
                     RMPQPESLPRTVRDSLPELAQQAEVRRQAGGNRDIPQPETIAPESETTVSTDREATVR
                     GGEVRGKKIEDFGEEIKGAAKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLA
                     NEGADADTLAMIALYRSDIPAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWM
                     EGRMPSRYADTWQLLRTLPPSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDN
                     KGSNLFFSESRDELLAKAKVWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDI
                     FIAYGKNKMRVRGGFKSASDARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGP
                     ERRKGNVSPEQFSDAFGFRGVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALS
                     LNGRLGLAFGARGKGKAAAHYESGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSN
                     DGKITSGGDFMTEAQRVRRIFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSA
                     LLDKVRSKPYWSTDVEMAARAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYP
                     TNAELDGGIREAFDHLFRTLKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGS
                     PVPQVEAVARGVMSGIKDSDLKVRVVKSQKEAEALAGELFDGYGRVHAFYRPDKREIV
                     LVADNIPDGRTVREKLRHEIIHHAMEHVVTPAEYQTIIKTVLKTRDSDNVTIREAWRK
                     VDASYGKESPEVQAGEFLAHMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDI
                     RLVRETIRTLGQRVREGYTPREDGAGASSQYSRSGKRDPFKVPEGEGERYRDDLARMM
                     KSLRTTDLTVNIGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPEL
                     MHDPDAIYRSATERNAVVMLLDAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENG
                     KKLKSMEMTGLTLYRREKLSRDNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYS
                     RTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAE
                     NDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGM
                     TNAEAAEIMQRVRNSGKQAQYDRLAGIIDDMLARRRELIREAGLEENGVVDAWQNAYR
                     YYVPLKGQDVDGVVSLPRTGKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRH
                     RKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRTIAERKDQETGETIREVVERPVPM
                     AMMADRYFTTKKNGKTYYIKLHDPRLMRAMKSMGPETSNAFVRTLGKVNRFLATVNTS
                     YNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASL
                     RGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIG
                     AWNSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGAL
                     MNSLYMFANASIQGTANLVRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLG
                     SLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLL
                     GHT"
BASE COUNT         1885 a         1765 c         2295 g         1505 t
ORIGIN      
        1 cgaatcgctg gaaggataac agtacgtcgt tcagcctggg cgatgtattg ccaacaatgg
       61 gtaaaggttt cgcccagtcc gtccggggaa caggggaaat ggcccgtgga ctcggtgatg
      121 cgatgattca gagcccggta aaaacagggg cgcgtatttt aaatgagttc agccgtatgg
      181 ggctgccggg tgtcgcaact gtgcaggata tttttgccgg tggcagcagg ggggctgatg
      241 aggtcatcga taccctgcct gatggcaaaa acgcggttac tgatactgtc ggtaaaggtc
      301 tgaaggcaac cggtaaggct gtcagtgatg gtgccaaagc cactgatgaa tggctgaccg
      361 gtaagatgtc gccgggtgca gttcgtgcgc tgaatacgcc gatgaccgaa ggctataatg
      421 attctgcggt ctgggtggcg aagggtgtaa acctgattgg tgcgcttgta cctgatatgg
      481 ttgctggcgg tgtggctaga aaggtgggtg atgtcacact gcgaaaaatg ctgaccgccg
      541 ggctggagaa aaaatacatc gcggcaggga tgcagccgga aagagccacg gcactggcag
      601 cagaagctgt cgataaaaaa atgccggatt tattccaggc gggcctgatc acccattcca
      661 ctgtaagtgc acaggggcag agtgcaatgg cggcagcaga tgctgttctt aatgctgatt
      721 actctgagct ggcgcagtca ccgaaatttc agcagacgtt tttgtccatt gacgccgacc
      781 cgcagcacgc acagcttact gatcgccaga aaatggatct ggcaaaagag cgtgttgccg
      841 atgaggtgcg cgcgcagctg gcaaccgatc ctgaattgct ggctgtgaat gccatggcgg
      901 caaaactggg tgacgcacaa ctgtttaatc tggtgacacg aggcacagcg aagaccgtta
      961 aaagcggcat tgtcagaaat gccacggaac agggggcgat taatgcggcg cagggcggct
     1021 attcacgcta tcaggaaaac acggcattgc gtgagaccgc cggaatgggt gtgtcaccgt
     1081 gggagggcgt ggctgacgca acgatcgaag gtgcagcctt tggtgctgcg atgggggctc
     1141 cattcggtgc ggttgccgga tatcgtggca gacgtcaggc cgcagaagaa accgccatgc
     1201 gtgatgctga aaccgtgcag caggacgacg cagccccgca accagaatct gttgatccgg
     1261 tggcgcagca gcgtgaatcc atgcagggca tgaatcgcga gcagcttctg gagcagtatg
     1321 ctgatgcgga tatggcaaca gagggtgacg catccgcagc tcatcgccgg gaagctgcca
     1381 gccagttgtt gaatgaactg gacgaacaga cgaagcgaca ggctgtgatg aatgagctga
     1441 aggcgaagcc gcgttctgaa ctgcttgagg aataccgcag actcagccag aaagaggggc
     1501 gcaccgagac tgaagaacaa cagtttcagg caatacgaga agtcattcgc ccacaacagg
     1561 aagtgacgcc ggaagcacag tcacagcctg aaaatgcgga ggatggtaac gggagcattt
     1621 acccgacggt gcggttccgg gacccgaatg aagtccgcat tgaaattaac gggaatggtg
     1681 cgtccagacc agcggaacgc attgagaagg tgcgcccgga caaccgttat ttcacggatg
     1741 agaaaagcgc catggggagt gatgttttcc gtaatgccgc cgccaccggc ctgaaaccgt
     1801 ccgtagtgaa gaaaggcgag aatcagtatg ccgttgaaat ggataatcct gcgttctctg
     1861 aagatgtggc aacggaaacc attaacaccc tggctgacgg agagcgtatt gctgatgctg
     1921 acccgatgga gcagcccgcg ttcatgcgtg acccgcgatt ccgtggtttc acgggggatg
     1981 atacggaggt acaggcccgc cttgcccgtg gcaacgcgcc gacggcagag gagcttgtac
     2041 gttcacagat ggctgaaggt gatgccggtc cgacagcaca ggagttaact gagcgtccac
     2101 gcctgcccgc tcccggcgat attcatcccg gacagggata tccgttaccg ggagaagtgg
     2161 cgcgtacgcc ggatgaaaat caggccggac gtggtggtcg ttttaccaca accggtgagg
     2221 ttaagggcca gagtttccag aaaggacaag ctccggcacc ggaaaacgcc gctggtcgcc
     2281 agggggaaac actcgagggt gacatggttc gtcgtggtct gccgtcaccg gatgcgcaga
     2341 acgcgacagc accggtacgt gaagggctac cggctcctga cattgcgcgt aatgttcgta
     2401 tgcctcagcc tgaatcactt ccccgcactg tacgggactc actgcctgag cttgcacagc
     2461 aggcagaagt acgccgacag gccggaggaa atcgtgacat cccgcagcct gagacaatcg
     2521 cacctgaatc tgaaacaact gtctctactg acagggaagc taccgtgcgc ggaggtgaag
     2581 tcaggggcaa aaaaattgaa gactttggtg aagaaattaa gggcgcggca aaacaccgtt
     2641 atgcacagct tgctgaaaca ctgggtaaaa cgctggaaga cagggattat gccacgcagc
     2701 cgctgagcaa actgttcccg aaaccggact acgcaaaact ggcgaacgaa ggtgctgatg
     2761 ctgataccct ggcaatgata gcgctgtatc gtagcgatat tccggcgaag acgaaacaca
     2821 atacggcagg ctggggggag agcataaaaa aagtacgaca cagtgtatcg gaaatgctga
     2881 acggaacggt cagcgcgaaa cgcctcgcag aatggatgga aggcagaatg ccctcccgtt
     2941 acgcggatac ctggcaactg ttacgcactc tgccaccctc acagatggac agggcttctg
     3001 cttatcgggt ggtatcgggt gtgtatcagg cggcaggagg gaagcgttac gatccgccac
     3061 agaaacttta ttcactgcgc aataaggaca ataaggggag taacctcttt ttctcggaaa
     3121 gcagggatga attactggca aaggcgaaag tctggtttgc agagcaggag gaaaaatcac
     3181 aggcgaaagg tgatgaaaaa acagcaccgt caccggatga caaaatccgc tttgacgttt
     3241 accggaatac ccgcagtggc gatattttta tcgcttacgg taaaaacaaa atgcgggtga
     3301 gaggtggctt taagtcagcc agtgatgcgc gtaagtacat tgattcacat cgtgatgagc
     3361 ttgttcgtca tgtgaaggag atgcgggaga tttcgcgtga ggagcagcgc aacgccacca
     3421 accgcgaccg taccggacca gaacgccgca aggggaatgt ttcaccggag cagttcagtg
     3481 atgcgtttgg tttccgtggt gtgcagtttg gtaactacgt ggaaggtccg cgtcgtcagg
     3541 ctgatttgaa ccgggcttat gactcgctgc atgaccttgc ggaagtactg aatgtaccga
     3601 caaaagcgct ttccctgaac ggtcgtcttg gcctggcatt tggtgcccgt ggtaagggta
     3661 aggcggcggc acactatgag tcaggtgagg tggcaatcaa cctgacaaaa ggtaacggac
     3721 cgggtgcgct ggcgcacgaa tggttccatt ctctggataa ttattttggt cgttatgacg
     3781 tttccaatga cgggaaaatt acgtcaggtg gcgactttat gacggaagca cagcgtgtca
     3841 ggcgcatatt taaagacggc aggtatgttg atgctgaata tccggtacgt caggaggttt
     3901 acgacgcttt taaaggtgtg attcaggcca ttaaaaacag tgacatgccg cgtcgttcag
     3961 cgcttctcga taaggtgcgc tcaaaaccgt actggtcaac ggatgttgaa atggcggcac
     4021 gtgcctttga gcgttatgtt caggataagg cgcgtatggc tggcgtggag aatgattatc
     4081 tggtcaatat ccgtaaggca cctgagcaca acacagataa cacctacgct tatccgacga
     4141 atgcggaact ggatggcggt attcgtgagg cattcgatca cctgttccgc accctgaaaa
     4201 cccgtgagac ggacaagggc gttgcgtttt attcccgtaa gggcgttacc cgcacacctg
     4261 aaggtaatct catttcggat gttaaccgta gtgcggaagc caaaggcagc ccggtcccgc
     4321 aggttgaagc ggttgcccgt ggcgtgatga gcggcattaa ggacagtgac ctgaaggtcc
     4381 gtgtggtgaa gtcacagaaa gaggctgaag cgctggcggg tgaattgttc gatggttacg
     4441 gcagggtgca cgcattctat cgtccggata aacgagaaat tgtcctggtg gcggataaca
     4501 tccctgacgg gcggaccgtt cgcgagaagc tgcgtcacga gatcattcac catgccatgg
     4561 agcatgttgt cacaccagcg gaatatcaga cgattatcaa aaccgtgctg aaaacccgcg
     4621 acagtgataa cgtcaccatc cgtgaagcct ggcgtaaggt tgatgcttcc tatggtaagg
     4681 aatcaccgga agtacaggcg ggtgaatttc tggcacatat ggcggagaaa cagccgaata
     4741 aattcgtggc ggcatgggag cgtgttgttg ccctggtcaa aggggtactg cgtcgtacgg
     4801 ggttactgaa gccgacggaa ctgaacgata tcagacttgt tcgcgagacc atccgtacgt
     4861 taggccagcg tgtgcgggaa ggttacacgc cgcgtgagga tggcgcgggc gcatcgtctc
     4921 agtactcccg tagtggtaaa cgtgatccgt tcaaagtgcc ggaaggtgag ggcgagcgtt
     4981 atcgtgatga ccttgccaga atgatgaaat ctctgcgcac cacagattta acggtaaaca
     5041 tcgggcgtac gccgccggta ttgcgtcacc ttggtgcacc ggatttgccg ctggttattt
     5101 cccgcgatac tgtgcggaag gccaccaatg gtgtgaaaca tgtggtgccg atggatgtta
     5161 tcgagagact accggaactg atgcacgatc cggatgcaat ttaccgctca gcgacagaaa
     5221 gaaatgcggt tgtgatgctg cttgatgccg tggataaaaa tggtgatccg gtggtgtcag
     5281 cggtacacat gaaggctgtc cggtcgcgtc tggaaatcaa caaggtagct tctgtttacg
     5341 gtacagaaaa tggaaaaaaa ctgaagagta tggaaatgac cgggttaacg ttgtaccgga
     5401 gagaaaaatt aagccgcgat aaccttctgc acagagggct ccaattgccc aaaggggaac
     5461 attcttatcg cggctctgcg gataaaatac tctatcctga agatattcgc aaggggccgt
     5521 attactcccg taccagcagt ctgacaccgg aagagacaat tgcatcgcgt tttgtgcgcc
     5581 agatgcagga taaattccag gtgctgaaag ctgttcagga gaatatccgt aaaactggcg
     5641 gcaaagtgga cgacagtaac aacgcttata tggcggaaga actcttccac gggaaggcgg
     5701 aaaacgacct gaacgtgatg aaggagcgct acgttcagcc actggctaaa ttactggcgg
     5761 actacaaaat tgcgcaggcc gatctggatg agtacctcta cgcccgtcac gcgccggaac
     5821 gtaacgcgca tatcgcgaaa atcaacccga aaatgccgga cggcggttcg gggatgacca
     5881 acgcggaagc ggcggaaatc atgcagcgtg tacgtaacag tggcaaacag gcacagtatg
     5941 accgtctggc agggattatt gacgatatgc tggcccgtcg ccgtgagctt atccgtgagg
     6001 ccggacttga agagaacggt gtggtggatg cctggcagaa cgcctaccgt tactacgttc
     6061 ccctgaaagg tcaggatgtt gacggtgtgg tgtcactgcc ccgtacaggt aagggcttca
     6121 ccatcggcgg acgtgaaagc aggcaggcca tggggcgtgc atcccgggca cagtctccgt
     6181 ccactcaggc gatacaggac ctgagcgaat cgctgatccg ccatcgcaaa aacgaagtgg
     6241 gtaacgcctt cctgaaactg gtgcaggata atcccgacaa ggattactgg caggtattca
     6301 ccgatgacag accggatacc atgcggacga ttgcagagcg caaggaccag gaaactggtg
     6361 aaaccattcg cgaagttgtc gaacgccctg taccgatggc aatgatggca gaccggtact
     6421 tcaccaccaa aaagaacggc aaaacgtact acatcaaact ccatgatccg cgcctgatgc
     6481 gtgcgatgaa gagtatggga ccggaaacca gcaatgcctt tgttcgtacg ctggggaaag
     6541 ttaaccgctt cctggcaacg gtgaacacgt cgtataaccc ggaattcctg gtcagtaact
     6601 tcatccgtga cgtgcagacg gcggtgatga acctgaaggc ggagcaggga aggagcgacg
     6661 gtaaactgaa agggctggat aacttatccg ccctggctgt ggtgaaagac agccgttctg
     6721 ccatgtcagc cgtatacgcc agtctgcgtg gtaaaaccct cacgggaaaa ggtgcacagt
     6781 ggcagaaggt gtggaaagag tttgttgagg acggagggaa aaccggctgg tttaacatgg
     6841 gtgaccttga aggccagcag aaggaaatgg atcgccttgt ctcactggcg aaggggggat
     6901 ggaaaggcca gagtatcggt gcatggaatt cgttccttaa ccttgtcgag gatgccaacg
     6961 gtgcggttga aaacgctctg cgtctttctg cctataagca cgcccgtgat gccggtttgt
     7021 cacgccagca ggcggcgtct cttgccaaaa acatgacggt gaactttaac cgtcgtggtg
     7081 agcagggggc gctgatgaat tcgctgtaca tgttcgccaa cgccagcatt cagggaacgg
     7141 caaatctggt gagaacgctc ggacatctta atggcgacgg gccgttactg gagcgccttc
     7201 gctggaagaa tctgaatgtt ccgcagaaaa tcgcgcttgc cgctgtggga gcaggttatc
     7261 tgcttggctc gcttaaccgc agcgtggcgg gtgaggatga tgacggggtt aactggtatg
     7321 acaaggtgcc gtctcatgtg aaagagcgta acctcgtcat tatgaaatcg gtgttcgggg
     7381 gcaaggccgg agagtactgg agtattcctc tgccttacgg gtacaacgtt ttcttcctgc
     7441 tcgggcatac
//
LOCUS       DAZGTR010000179         7420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGTR010000179 DAZGTR010000000
VERSION     DAZGTR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.181"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>7420
                     /locus_tag="ACHLCY_004497"
     CDS             <1..>7420
                     /locus_tag="ACHLCY_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFP0371600.1"
                     /translation="NRWKDNSTSFSLGDVLPTMGKGFARSVRGTGEMARGLGDAMIQS
                     PVKTGARILNEFSRMGLPGVATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKV
                     TGKAVSDGAKSSDEWLTSKMSPGALRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMV
                     AGGVAKKVGDVTLRKTLTAGLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITH
                     STASAQGQSAMAAADAVLNADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKE
                     RVADEVRAQLATDPELLAVNAMAAKLGDAQLLNLAMRGTANTVKSGMIRNATEQGAIN
                     AAQGGYSRYQENMALRDTAGMDVSPWEGVADATIEGGTLGAAMGAPFGAVAGYRGRRQ
                     AAEETAMRDAETVQQGEAAPQPEPVDPVAQHRESMQGMNREQLLEQYADADMAHEGDT
                     SAVHRREAASQLLNELDEQAKRQAVMDELKAKPRPELLEEYRKLSLKEGRTDTEEQQL
                     QAIRDVLRPQQEARPEAQPQPENADDGDGSIYPTVRFRDPDEVRIEINGSGASRPAER
                     IEKVRPDNSYFTDEKSALGSDVFLNASATGVKPSVVKKGENQYAVEMDNPAFSEDVAT
                     ETINTLADGERITDADPMEQPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQ
                     MAEGDAGPTAQELTERPRLPAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEV
                     KGQSFQKGRAQAPENAAGRQGEILEGEPVRRGLPSPDEQNATAPVREGLPAPESQRGV
                     DMPQPESLPRIVRDSLPELAQQAEARRQTGDNRQTITDVPDTEVPVPVDKPSTHQQAR
                     GAKIEDFGEEIKGAAKHRYAQLAEAMGKTLEDGEYATQPLSKLFPKPDYARLEKEGVD
                     SDTLAMMALYRSEIPTRTSRNMQKWISIVKSGREATAGMLEGKIPAAKLADMMDNKSG
                     LRSMSDTWKLLRTLSASQIDKASGYRVRSGVYSFVGGKRYDPPQMMYSLRDSKGRDLF
                     FSESRDELLKKAKAYFDEQGSRERETPATSADDRITFDVYRHKASGDIFIGYGKNRQK
                     LKGGFESARDAHDYVRTHRDELVNQVKALREVSREEQRNATNRDRTGPERRKGDVSPE
                     QFSDAFGFRGVQFGNYVEGPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFG
                     ARGKGKAAAHYEPGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDY
                     MTEAQRAGRVFKDGRYVDAEYPVRQEVYDAFKGVMKAINSSDMLRRSELLDGARSKPY
                     WSTDVEMAARAFERYVQDKARMAGVENDYLVNIRKADDHGQPDTYAYPTNAELDGGIR
                     EAFDHLFRTLKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVAR
                     GVISGIKDSDLKVRVVKSQKEAEALAGESFDGYGKVHAFYRPDKREIVLVADNIPDGR
                     TVREKLRHEIIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESP
                     EVQAGEFLAHMAEKQPNKFVAAWVRVVALVKGVLRRTGLLKPTELNDIRLVRETIRTL
                     GQRVREGYTPREDGADASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRSGAMTA
                     NIGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHNPDAIYRS
                     ATERNAVVMLLDAVDKNGDPVVSAVHMKATDKRLEINKVASVYGTENGKKLKSMEMAG
                     LTLYRKEKLSRDNPQYSGLQLPKEERSYRGSVDKILYPEDIRKGPYYSRTSSLTPEET
                     IASRFVRQMQDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERY
                     VQPLVKLLADYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQ
                     RVRNSGKQAQYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQKAYRYYVPLKGQDV
                     DGVVSLPRTGKGFTIGGRESKQAMGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFL
                     KLVQDNPDKDYWQVFTDDRPDTMRAIAERKDQETGETIREVVERPVAMAMMADRYFTT
                     KKNGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNF
                     IRDVQTAVMNLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGA
                     QWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVE
                     DANGAVENALRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANA
                     SIQGTANLVRTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGED
                     DDGVNWYDKVPDHVKERNLVIMKSMFGGKAGEYWSIPLPYGYNVFFLLGHT"
BASE COUNT         1836 a         1729 c         2319 g         1536 t
ORIGIN      
        1 caaatcgctg gaaggataac agtacgtcgt tcagcctggg cgatgtattg ccaacaatgg
       61 gtaaaggttt cgcccggtcc gtccggggaa caggggaaat ggcccgtgga ctcggtgatg
      121 cgatgattca gagcccggta aaaacagggg cgcgtatttt aaatgagttc agccgtatgg
      181 ggctgccggg tgtcgcaact gtgcaggata tttttgccgg tggcagcagg ggggctgatg
      241 aggtcatcga taccctgcct gatggcaaaa acgcggttac agatactgtc ggtaaaggtc
      301 tgaaggtcac cggtaaggct gtcagtgacg gcgctaaatc ctcggatgaa tggctgacca
      361 gtaagatgtc tccgggtgct ctgcgtgcgc tgaatacgcc gatgaccgaa ggctatgatg
      421 attctgcggt ctgggtggcg aagggcgtaa acctgattgg tgcccttgta cctgatatgg
      481 ttgctggcgg tgtggctaaa aaggtgggtg atgtcacatt gcgaaaaacg ctgaccgccg
      541 ggctggagaa aaaatacatc gcggcaggga tgcagccgga aagagccacg gcactggcag
      601 cagaagctgt cgataaaaaa atgccggatt tattccaggc gggcctgatc acccattcca
      661 cagccagtgc acaggggcag agtgcaatgg cggcagcaga tgctgttctt aatgcggatt
      721 actctgagct ggcgcagtca ccgaaatttc agcagacgtt tttgtccatt gacgccgatc
      781 cgcagcacgc acagcttact gatcgccaga aaatggatct ggcaaaagag cgtgttgccg
      841 atgaggtgcg cgcgcagctg gcaacagatc ctgaattgct ggctgtgaat gccatggcgg
      901 caaaactggg cgatgctcag ttgcttaatc tggcgatgcg gggcacggcg aataccgtta
      961 aaagcggcat gatcagaaac gccactgaac agggggcgat taatgcggcg cagggcggct
     1021 attcacgcta tcaggaaaac atggcactgc gtgataccgc cggaatggat gtgtcaccgt
     1081 gggagggcgt ggctgacgca acgatcgaag gtggtactct tggtgctgcg atgggggctc
     1141 cattcggtgc ggttgccgga tatcgtggca gacgtcaggc cgcagaagaa accgccatgc
     1201 gtgatgctga aaccgtgcag cagggcgaag cggccccgca acccgaaccg gttgatccgg
     1261 tggcgcagca ccgtgaatcc atgcagggga tgaatcgcga gcagcttctg gaacagtatg
     1321 ctgatgcgga tatggcgcat gagggagaca cgtctgccgt tcatcgccgg gaagccgcca
     1381 gccagctgtt gaatgaactg gatgaacagg cgaagcgaca ggcggtgatg gatgagctga
     1441 aggcgaagcc gcgccctgaa ctgcttgagg aataccgaaa actcagcctg aaggaagggc
     1501 gtactgatac tgaagagcag cagttacagg ctatccgtga tgtgcttcgc cctcagcagg
     1561 aggccagacc ggaggcacag ccacagccgg aaaatgcgga tgatggcgac gggagtattt
     1621 acccgacggt gcggttccgc gatccggatg aagttcgtat tgaaattaac gggagtggtg
     1681 cgtccagacc agcagaacgc attgaaaagg tgcgtccgga caacagttat ttcacggatg
     1741 aaaaaagtgc tctggggagc gacgttttcc tcaatgccag cgccaccggc gtgaaaccgt
     1801 ccgtagtgaa gaaaggcgag aatcagtatg ccgtagaaat ggataatcct gcgttctctg
     1861 aagatgtggc aacggagacc attaacactc tggctgacgg agagcgtatt actgatgctg
     1921 atccgatgga acagcccgcg ttcatgcgtg acccgcgatt ccgtggtttt acgggggatg
     1981 atacagaagt gcaggcccgt cttgcccgtg gcaacgcgcc gacggcggaa gaactggtgc
     2041 gttcccagat ggctgaaggt gatgccggtc cgacagcaca ggagttaact gagcgtccac
     2101 gccttcccgc tcccggcgat attcatcccg gacagggata tccgttaccg ggagaagtgg
     2161 cgcgtacgcc ggatgaaaat caggccggac gtggtggtcg ttttaccaca accggtgagg
     2221 tgaagggcca gagtttccag aaaggacgtg cgcaggcacc ggaaaatgcc gctggtcgcc
     2281 agggggaaat tctggagggc gaaccggttc gtcgtggtct gccgtcaccg gatgagcaga
     2341 acgccacagc accagtgcgt gaaggacttc ctgcacctga aagtcagcgt ggggttgata
     2401 tgccacagcc tgaatcactc cctcgtatag ttcgtgactc ccttccggaa ctggcacagc
     2461 aggcagaagc acgcagacag accggggata atcgccagac cataaccgat gttccggata
     2521 ctgaggtgcc ggtgccggta gataaaccga gcactcacca gcaggcgcgt ggtgcgaaaa
     2581 ttgaagactt tggcgaggag attaagggcg cggcaaaaca ccgttatgcg cagcttgctg
     2641 aagctatggg taaaacgctg gaggatgggg agtacgcgac acaaccactg agcaaactgt
     2701 ttccaaagcc ggattatgcc cgtctggaaa aagaaggtgt ggatagtgac acgctggcaa
     2761 tgatggcgtt gtatcgcagt gaaattccca ccagaacaag ccgtaacatg cagaaatgga
     2821 tcagcattgt taaaagcggt cgcgaagcta ctgccggtat gctggagggg aaaattccgg
     2881 cagcgaaact ggctgatatg atggacaata agtcggggtt acggagcatg tcggatacct
     2941 ggaaactgct ccgtacgctt tctgcatcgc agattgataa agcatccggc tatcgtgtca
     3001 ggtccggtgt ttattcgttt gtcgggggaa aacgttacga tccgccgcaa atgatgtatt
     3061 cgcttcgtga tagcaagggg cgcgacctct ttttctcgga aagccgggat gagttactga
     3121 aaaaggcgaa agcatatttc gatgaacaag gctccagaga aagggaaacg ccagcaacct
     3181 ctgctgatga cagaataacc tttgacgtgt accggcataa ggccagtggc gacattttta
     3241 tcggttatgg caaaaacagg cagaaactga agggcggttt tgagtctgcc cgtgatgcac
     3301 atgattatgt gcgcacacat cgtgacgagc tggttaatca agtgaaggcg ctgcgtgaag
     3361 tctcgcgtga ggagcagcgt aacgccacca accgcgatcg taccggacca gaacgccgca
     3421 agggggatgt ttcgccggag cagttcagtg atgcgtttgg ttttcgtggt gtgcagtttg
     3481 gtaattatgt ggaaggtccg cgtcgtcagg ctgatttgaa ccgggcttat gactcgctgc
     3541 atgacctggc tgacgtgctg aacgtgccga caaaagcgct ttctctgaac ggtcgtcttg
     3601 ggcttgcttt tggtgcccgt ggtaagggta aggcggcggc acactatgag ccgggtgagg
     3661 tggcaatcaa cctgacaaaa ggtaacggac cgggggcgct ggcccacgaa tggttccatt
     3721 cactggataa ttattttggt cgttatgatg tttccactga cgggaagatc acgtcaggtg
     3781 gcgactatat gacggaagca cagcgtgccg ggcgcgtatt taaagacggc aggtatgttg
     3841 atgcggaata tccggtacgt caggaggttt acgacgcgtt taaaggcgtg atgaaagcca
     3901 ttaacagcag tgacatgttg cgtcgttctg aactcctgga tggcgctcgt tcaaaaccgt
     3961 actggtcaac ggatgttgaa atggcggcgc gtgcctttga gcgttatgtt caggataaag
     4021 cgcgtatggc tggcgtggag aatgattatc tggtcaatat ccgtaaggcg gatgaccatg
     4081 gacagccgga cacctacgct tatccgacga atgcagaact ggatggcggt attcgtgagg
     4141 cattcgatca cttgttccga acactgaaaa cccgtgagac ggacaaggga gttgcgtttt
     4201 attcccgtaa gggcgtgacc cgcacacctg aaggcaatct gatttcggat gttaaccgca
     4261 gtgcggaagc caaaggcagt ccggtcccgc aggttgaagc tgttgcccgt ggcgtgatta
     4321 gtggcattaa ggacagtgac ctgaaggtcc gcgtggtgaa gtcacagaaa gaggctgaag
     4381 cactggcggg tgaatcattc gacggttacg gcaaggtgca cgcgttctat cgtccggata
     4441 aacgtgaaat tgtcctggtg gcggataaca tccctgacgg gcggaccgtt cgcgagaagt
     4501 tacgtcacga gattattcac catgcaatgg agcatgtagt cacgccagcg gaatatcaga
     4561 cgattattaa gaccgtgctg aaaacccgtg acagtgataa cgccaccatc cgtgaagcct
     4621 ggcgtaaggt tgatgcgtct tatggtaagg aatcaccgga agtgcaggcg ggtgaatttc
     4681 tggcgcatat ggcagagaag cagccgaata aattcgtggc ggcatgggtg cgtgttgttg
     4741 ccctggtcaa aggggtactg cgtcgtacgg ggttactgaa gccgacggaa ctgaacgata
     4801 tcagacttgt tcgcgaaact attcgcacgt taggccagcg tgtgcgggaa ggttacacgc
     4861 cgcgtgagga tggcgcggac gcatcgtctc agtactcccg tagtggtaaa cctgatccgt
     4921 tcaaagtgcc ggaaggtgag ggcgagcgtt atcgtgatga ccttgccaga atgatgaagt
     4981 cgttacgttc tggtgcaatg actgcaaaca tcgggcgtac gccgccggta ttgcgccacc
     5041 ttggcgctcc agatttgccg ctggttatct cccgcgacac tgtgcggaag gctaccaatg
     5101 gcgtgaaaca tgtggtgcca atggatgtta tcgagagact tccggaactg atgcacaatc
     5161 cggatgcaat ttaccgttcc gcgacagaaa gaaatgcggt tgtgatgctg cttgatgctg
     5221 tggataaaaa tggtgatccg gtcgtctcgg cagtgcatat gaaggcgaca gataaacgtc
     5281 tggaaattaa caaggtggca tctgtttacg gtacggaaaa cgggaaaaag ctgaaaagta
     5341 tggaaatggc tggattaacg ctttaccgga aggagaaatt aagccgcgat aaccctcagt
     5401 acagtgggct ccaattgccc aaagaggagc gttcttatcg cggctctgta gataaaatac
     5461 tctatcctga agatattcgc aaggggccgt attactcccg taccagcagt ctgacgccgg
     5521 aagagacaat tgcatcgcgt tttgtgcgcc agatgcagga taaattccag gtgctgaaag
     5581 ctgttcagga gaatatccgt aaaactggcg gaaaaataga cgacagtaac aacgcttata
     5641 tggcggaaga actcttccac gggaaggcgg aaaacgacct gaacgtgatg aaggagcgtt
     5701 acgttcagcc actggttaaa ttactggcgg actacaaaat tgcgcaggcc gatctggatg
     5761 agtacctcta cgcccgtcac gcaccggaac gtaacgcgca tatcgcgaaa atcaacccga
     5821 aaatgccgga cggcggttcg gggatgacca acgcggaggc ggcggaaatc atgcagcgtg
     5881 tgcgtaacag cggcaaacag gcacagtatg accgtctggc agggattgtt gacgatatgc
     5941 tggcccgtcg ccgtgagctt atccgtgagg ccggactgga agagagcggc gtggtggatg
     6001 cctggcagaa agcctaccgt tactacgttc ccctgaaagg gcaggatgtt gacggtgtgg
     6061 tgtcactgcc ccgtacaggt aagggcttca ccatcggcgg gcgtgaaagt aagcaggcca
     6121 tggggcgtgc atcccgcgca cagtctcctt ccactcaggc gatacaggat ttgagtgaat
     6181 cgctgatccg caatcgcaaa aacgaagtgg gtaacgcctt cctgaaactg gtgcaggata
     6241 atcccgataa ggattactgg caggtattca ctgatgacag accggatacc atgcgtgcga
     6301 ttgcagagcg caaggaccag gaaactggtg aaaccattcg cgaagttgtc gagcgtccgg
     6361 tggcgatggc aatgatggca gaccggtact tcaccaccaa aaagaacggc aaaacttact
     6421 acatcaaact ccatgacccg cgcctgatgc gtgcgatgaa gaacatgggg ccggaaacca
     6481 gtaacgccgt aatccgtacg ctggggaaag ttaaccgctt cctggcaacg gtgaacacgt
     6541 cgtataaccc ggaatttctg gtcagtaact tcatccgtga cgtgcagacg gcggtgatga
     6601 acctgaaggc ggagcaggga aggagcgacg gcaaactgaa agggctggat aacttatccg
     6661 ccctggctgt ggtgaaagac agccgttccg ccatgtcagc cgtatacgcc agtctgcggg
     6721 gtaaaaccct cacgggcaaa ggcgcacagt ggcagaaggt gtggaaagag tttgttgagg
     6781 atggaggtaa aaccggctgg tttaacatgg gtgaccttga aggccagcag aaggaaatgg
     6841 atcgccttgt atcgctggcg aaggggggat ggaaaggcca gagtatcggt gcatggaatt
     6901 cgttcctgaa ccttgtggag gatgccaacg gtgcggttga aaacgcactg cgtctttctg
     6961 cctataagca cgcccgtgat gccggtttgt cacgccagca ggcggcgtct cttgccaaaa
     7021 acatgacggt gaactttaac cgtcgtggtg agcagggggc gctgatgaac tcgctgtaca
     7081 tgttcgctaa cgccagcatt caggggacgg caaaccttgt gagaaccctt gggcacctta
     7141 atggtgacgg tccgttactg gagcgccttc gctggaagaa tctgaatgta cctcagaaaa
     7201 tcgcgcttgc tgctgtggga gcgggttatc tgcttggctc gcttaatcgg agtgtggcgg
     7261 gtgaggatga tgacggggtt aactggtatg acaaggtgcc tgatcatgtg aaagagcgca
     7321 acctcgttat tatgaaatcg atgttcgggg gcaaagccgg tgagtactgg agtattcctc
     7381 tgccttacgg gtacaacgtt ttcttcctgc tcgggcatac
//
LOCUS       DAZGTR010000180         7377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGTR010000180 DAZGTR010000000
VERSION     DAZGTR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.182"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            257..4505
                     /gene="fdeC"
                     /locus_tag="ACHLCY_004498"
                     /pseudo
     CDS             257..4505
                     /gene="fdeC"
                     /locus_tag="ACHLCY_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
     gene            complement(4626..5483)
                     /locus_tag="ACHLCY_004499"
     CDS             complement(4626..5483)
                     /locus_tag="ACHLCY_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371601.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            5732..6601
                     /locus_tag="ACHLCY_004500"
     CDS             5732..6601
                     /locus_tag="ACHLCY_004500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0371602.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQREVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6761..7354)
                     /gene="rclC"
                     /locus_tag="ACHLCY_004501"
     CDS             complement(6761..7354)
                     /gene="rclC"
                     /locus_tag="ACHLCY_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP0371603.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIFFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQPSNESSSTLKTEY"
BASE COUNT         2022 a         1651 c         1892 g         1812 t
ORIGIN      
        1 taacttaggt atctatttaa tgacttgcac aaaaagctaa atttcccccc ataaataaaa
       61 atataatccc gcgcccaacc acctgatgag tggctacagg cactggatat attaggtggc
      121 ggtgcacttt cttacataaa ggtatttcct tttctgcgga aaaggaaatc gggaaatccc
      181 cggtttttct gacaagcaga cgccattatt tgtgtctgcc tatgttcgtt aattcgttca
      241 tcaggaaatt atctcaatgt cacattataa aacaggtcat aaacaaccac gatttcgtta
      301 ttcagttctg acccgctgcg tggcgtgggc aaatatctct gttcaggttc tttttccact
      361 cgctgtcacc tttaccccag taatggcggc acgtgcgcag catgcggttc agccacggtt
      421 gagcatggga aatactacgg taactgctga taataacgtg gagaaaaatg tcgcgtcgtt
      481 tgccgcaaat gccgggacat ttttaagcag tcagccagat agcgatgcga cacgtaactt
      541 tattaccgga atggccacag ctaaagctaa ccaggaaata caggagtggc tcgggaaata
      601 tggtactgcg cgcgtcaaac tgaatgtcga taaagatttc tcgctgaagg attcttcgct
      661 ggaaatgctt tatccgattt atgatacgcc aacaaatatg ttgttcactc agggagcaat
      721 acatcgtacc gacgatcgta ctcagtcaaa tattggtttt ggctggcgtc atttttcagg
      781 aaatgactgg atggcggggg tgaacacctt tatcgaccat gatttatccc gtagtcatac
      841 ccgcattggt gttggtgcgg aatactggcg cgattatctg aaactgagcg ccaatggtta
      901 tatccgggct tctggctgga aaaaatcgcc ggatgttgag gattatcagg aacgcccggc
      961 gaatggctgg gatattcgtg ctgagggcta tttacccgcc tggccgcagc ttggcgcaag
     1021 cctgatgtat gaacagtatt atggcgatga agtcgggctg tttggtaaag ataagcgcca
     1081 gaaagacccg catgctattt ctgccgaggt gacctatacg ccagtgcctc ttctgacact
     1141 gagcgccggg cataagcagg gcaagagtgg tgagaatgac actcgctttg gcctggaagt
     1201 taattatcgg attggcgaac ctctggcgaa acaactcgat acagacagca ttcgcgagcg
     1261 tcgggtactg gcaggcagcc gctatgacct ggttgagcgt aataacaaca tcgttcttga
     1321 gtaccgcaaa tctgaagtga tccgtattgc tctgcctgaa cgtattgaag gtaagggtgg
     1381 tcagacactt tccctggggc ttgtggtcag caaagcaact cacggactga aaaatgtgca
     1441 gtgggaagcg ccgtcattac tggctgaggg tggcaaaatt accggtcagg gtagtcagtg
     1501 gcaagtaacg ctcccggctt atcgtccagg caaagacaat tattatgcga tttctgcggt
     1561 tgcctacgat aacaaaggca atgcctcaaa acgcgtgcag acagaggtgg tcattaccgg
     1621 agcaggtatg agcgccgatc gcacggcgtt aacgcttgac ggtcagagcc gtattcaaat
     1681 gcttgctaac ggtaatgagc aaagaccgct ggtgctgtct ctgcgcgacg ccgaggggca
     1741 gccagtcacg ggcatgaaag atcaagactg aactagcctt caaaccggct ggaaatattg
     1801 tgactcgttc cctaaaggcc actaaatcac aggcaaagcc aacactgggt gagttcaccg
     1861 aaactgaagc aggggtgtat cagtctgtct ttactaccgg aacgcagtca ggtgaggcaa
     1921 cgattactgt tagcgttgat ggcatgagca aaaccgtcac tgcagaactg cgggccacga
     1981 tgatggatgt ggcaaactcc accctgagcg ctaacgagcc gtcaggtgat gtggttgctg
     2041 atggtcagca agcctatacg ttgacgttga ctgcggtgga ctccgagggt aatccggtga
     2101 cgggagaagc cagccgcttg cgatttgttc cgcaagacac taatggtgta accgttggtg
     2161 ccatttcgga aataaaacca ggcgtttaca gcgccacggt ttcttcaacc cgtgccggaa
     2221 acgttgttgt gcgtgctttc agcgagcagt atcagctggg cacattacaa caaacgctga
     2281 agtttgttgc cgggccgctt gatgcagcac attcgtccat cacactgaat cctgataaac
     2341 cggtggttgg cggtacagtt acggcaatct ggacggcaaa agatgctaat gacaaccctg
     2401 taactggtct caatccggat gcaccgtcat tatcgggcgc agctgctgta ggttctacgg
     2461 catcaggctg gacggataat ggcgacggga cctggactgc gcagatttct ctcggcacta
     2521 cggcgggtga attagaggtt atgccgaagc taaatggaca ggatgcggca gcgaatgcgg
     2581 ctaaagtaac cgtggtggct gatgcgttat cttcaggcca gtcgacagtg tctgtagccg
     2641 cagatcgcgt aaaagcaggc gaaagcacaa ccgtgacgct tattgcaaaa gatgcacatg
     2701 gcaacgctat cagtggtctt tccctgtcgg caagcctgac gggtgctgcg tctgaagggg
     2761 cgactgtttc tggttggacc gaaaaaggtg atggttccta tgtcgctaca ctgacaacag
     2821 gtggaaagac gggtgagctt ctcgtcatgc cgctattcaa cggccagcca gcagccaccg
     2881 aagccgcgca gttgactgtc attgcggggg agatgtcatc agcgaactct acgcttgttg
     2941 ctgacaataa ggctccgacc atcaaaacga cgacgaaact caccttcacc gtgaaggatg
     3001 cgtacgggaa ccttgtcacc gggctgaagc cagatgcacc gcagtttagt ggtgccgcca
     3061 gcacggggac agagcgacct tcaacaggag actggacaga aacaagtaat ggggtctacg
     3121 tggcgacctt aacgctggga tctgccgcgg gccagttgtc tgtgatgccg cgagtgaacg
     3181 gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc tggtgacgca tctaaggctg
     3241 agattcgtga tatgacggtg aaggttgata accagctggc taatggacaa tcgactaacc
     3301 aggtaaccct gaccgttgtg gacacctatg gtaacccgtt gcagggacaa aatgtgacgc
     3361 tgactctgcc gaaaggtgtg accagcaaga cggggaatac ggtaacaacc gatgcggcag
     3421 gtaaagccga cattgagctg atgtcaacgg ttgccgggga acacagcatc acggcctcag
     3481 tgaataatgc tcagaagacg gtcacggtga aattcaacgc ggatgccagc accggtcagg
     3541 caaacctgca ggtagacacc gctgctcaaa aagtggcaaa cggcaaagat gcctttacgc
     3601 tgacggcgaa cgttgaggat aaaaatggta accctgttcc agggagcctg gtgaccttta
     3661 atctgccccg gggtgtcaag ccgcttacag gcgataatgt ctgggtgaaa gccaacgatg
     3721 aggggaaagc cgagttgcag gtggtttcag tgactgccgg aacgtatgag atcacggcat
     3781 cggcagggaa tagccagcct tcgaatacgc agactataac gtttgtagcc gataaggcta
     3841 ccgcaaccgt ctccggtatt gaggtgatgg gcaactatgc gctggcggac ggcaaagcca
     3901 aacaaacgta taaagttacg gtgactgatg ccaataacaa tttggtgaaa gatagcgaag
     3961 tgacgctgac tgccagcccg gcttcgttaa acctggaacc gaatggcact gcgacaacga
     4021 atgagcaagg gcaggctatt ttcaccgcta ccactactgt tgcggcgaca tacacactca
     4081 aggcgcaagt gagtcagacc aacggtcagg tatcaacgaa aactgccgaa tctaaattcg
     4141 ttgcggatga taaaaacgcg gtactcaccg catcatctga tatgcaatct ctggtggcgg
     4201 atgggaaatc gactgcgaag ctggaggtga cactgatgtc ggcaaacaac cccgttggcg
     4261 ggaatatgtg ggtcgacatt cagacgccgg aaggggtgac ggaaaaggat tatcagttcc
     4321 tgccgtcgaa aaatgaccat ttcgtgagcg gaaaaatcac gcgtaaattt agtaccagca
     4381 agcctggtgt ctatacgttc acatttaacg ccctgaccta tggcgggtac gaaatgaagc
     4441 cagtgacggt gaccattacc gcggtggatg ccgatacggc aaaggacgag gaggcgatga
     4501 aataatttta agaatatgaa agtaacattc taataaacaa taatggcgtt gacatcttca
     4561 acgccattat ttattgaatt tcctgacagt agtctcgtta taaattcatt tagatatagc
     4621 gaaaactaaa ccggacgaac ggggataaaa atttcgcaaa caatgttatt gctatcaatt
     4681 tcgacgaaat tttcattgta ataaaaccgc tcaatatcac tgccgttcac tctggctaac
     4741 cccctggcgg gtaattccat cagatagagg tttcgcgccc attcagggta gtgctcccac
     4801 tcaccttcat agcggaaaga ggcatacagt cctcggttaa ttgtccactc tttcggcatc
     4861 tctgaatcgg tttgtttgca taccgtaccg gcaaaagttt caaccgtgag caaatcgacc
     4921 gattttgttg acggttcaaa cgtggagacg aaatagatat ccgtcttaaa attattcata
     4981 catctggcta aatgatgacg tcgcagtttg acttcttcat ccggtgattt attcgacact
     5041 tcaatatcat atttaaatga attgccaaaa ataggtgtct caggaaagtt gactaatcta
     5101 cactcggtct tttgctgttg acgtattaaa aaagaaggga agatatttgc gagatcccaa
     5161 taatcacggt ggcggtatac acggggagag cagccgaata actttttaaa ttcgcggctg
     5221 aatgactgct gtgaatcaaa atgcaaagag agtgcaatat caagcataga tttcgcggtg
     5281 agccggacaa gaatggcggc acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc
     5341 acatgcatga aatttcggaa cagaagctgg atgttgcgtc tgctgtagcc agatttctgt
     5401 gcgatatctt cgattgaaat agggtgctca agattgcact caatccactc caggagctgc
     5461 tgtagaatct tctgcctgat catcacattc tccattgatt cttcgtcata tcgcacattt
     5521 tcgttatgcc ctgggcccta agtgtatcaa aaacagcaag ggcgaaggca aaagcactgg
     5581 tagcgtctgc ttcgttgtcc ccgaaaatag agaggtttca gtcctgcatt attaatgaat
     5641 tttttgcata agtgatatca aaatccacgt actaatttga ggttacgttt taacgtagac
     5701 tcattgttca tgcctaatgg agggactgac agtggaattt tccgtattaa gtaacaatct
     5761 gaaaatgccg atgatgggat ttggtgtttt tcaggttacc gataaaaacg tgtgcaaaca
     5821 gtcagtgttg aacgctatcc gcacaggtta tcgactcatc gacactgcag cggtatacgg
     5881 taatgaagat gccgtcggtg aagccgttcg tgaagctata tctgaaggtt tatgtacccg
     5941 agatgagtta tttataacgt ctaaattgtg ggtgcaggat atgttgaatc aggatacggc
     6001 agcagcaggt attgaagcat cattaaaaaa atcgggacta gagtacttcg acctctattt
     6061 attgcaccag gctatgcgcg attatttcag tgcgtggcgt gcactcgaag atgcctatga
     6121 agagggcaaa ttaaaagcaa ttggggtttc caatttctat cctcatgttc tggcgaactt
     6181 ttgtgaaacg gtaagagtta aaccgatggt caaccaggtc gagttgcatc cctattttgc
     6241 ccaaccagag gcgctggcaa ccatgaagta ttataacgtg cagcctgaag catgggctcc
     6301 gttaggtggt ggacgacata aaccctttga aaataatctg cttcagagta ttgcagatgc
     6361 ccatcaaaaa tcgatttctc aagtcattct gcgttggaat attcaacggg aagtggtcgt
     6421 tattccgaaa tcgacacatc aacagcgtat cgaagaaaat tttgctatct gggatttctc
     6481 actgacagag aaagaaatgg cacaaattag ttcgcttgat ttgggttatg ttggggagtc
     6541 ggtaaaacat tttaatcctg aatttgttcg tggttgtctt gctgtaaaaa tacatgattg
     6601 atattaatca tatattttac ctgagacgac aagaatcttt taacagggga gtgatattga
     6661 tcttcactct gtcatatctc cggtaatatg gcgtcaggct tcatcatcag aaagggggcc
     6721 tgacgcctga aaaagtgaac aacagacagt gttcggatta tcaatattca gtttttaacg
     6781 ttgaactgga ttcattactg ggttgtttaa gaatttcccg cgccgaatct gccattatca
     6841 tgacagcacc tgccagcatc agagtatctt tcaataccag gcgaccagca ccggataaat
     6901 aagggaaacc atgatgagcg tcacccaatg cgggtaccca tgcctccggg gtggtgatta
     6961 aaaatgagag tgttaccagc ggcgtggtaa atgccatcag tcctcccaat aaacctaacc
     7021 agcgattgac aggattagcc aaaaccagca gagcaataat cacctccacg acgccaagac
     7081 cgttggaaaa accataggta ttattggccg tttgccatgc ccttgcttct ggtttgtatt
     7141 cgccttcgtg agtcagatac tgtttatagt cttccgggtg ttcataaaag aacgacatta
     7201 gtggactgtt tgcgacgaat ggtgtaatgc tgtctgcctc gtaagggaca aactttaata
     7261 acccaatcca cataaaaaaa attgcaatac tcagacgaat caatgtcagg ccaattttat
     7321 ctccccgact taacaggtgc aggtattttt ccattgtgaa catccttatt tatacaa
//
LOCUS       DAZGTR010000181         7353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGTR010000181 DAZGTR010000000
VERSION     DAZGTR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.183"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            155..270
                     /gene="rrf"
                     /locus_tag="ACHLCY_004502"
     rRNA            155..270
                     /gene="rrf"
                     /locus_tag="ACHLCY_004502"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(385..828)
                     /locus_tag="ACHLCY_004503"
     CDS             complement(385..828)
                     /locus_tag="ACHLCY_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0371604.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            985..1914
                     /gene="metA"
                     /locus_tag="ACHLCY_004504"
     CDS             985..1914
                     /gene="metA"
                     /locus_tag="ACHLCY_004504"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP0371605.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2183..3784
                     /gene="aceB"
                     /locus_tag="ACHLCY_004505"
     CDS             2183..3784
                     /gene="aceB"
                     /locus_tag="ACHLCY_004505"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP0371606.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3814..5118
                     /gene="aceA"
                     /locus_tag="ACHLCY_004506"
     CDS             3814..5118
                     /gene="aceA"
                     /locus_tag="ACHLCY_004506"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP0371607.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            5399..7135
                     /gene="aceK"
                     /locus_tag="ACHLCY_004507"
     CDS             5399..7135
                     /gene="aceK"
                     /locus_tag="ACHLCY_004507"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP0371608.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTNDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(7104..>7353)
                     /locus_tag="ACHLCY_004508"
     CDS             complement(7104..>7353)
                     /locus_tag="ACHLCY_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371609.1"
                     /translation="NHARLFGFTAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELIL
                     NTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
BASE COUNT         1791 a         1904 c         1903 g         1755 t
ORIGIN      
        1 tgtgtaagcg cagcgatgcg ttgagctaac cggtactaat gaaccgtgag gcttaacctt
       61 acaacgccga aggtgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
      121 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggccttagcg cggtggtccc
      181 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      241 tccccatgcg agagtaggga actgccaggc atcaaattaa gcgaaaggcc atcagtcacg
      301 atggcctttt tgcgttggtg aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc
      361 ctgggcaggc acggcagggg cggcttattc ccccacatac gccagattca gcaacggata
      421 cggtttcccc aaatcgtcca cctcagagcg tcccgtaacc ttaaaaccca ccttcttata
      481 gaacccaacc gcctgctcat tttgctcatt aacgttggtt gtcagttccg gtgccatcga
      541 gagcgcatgc tccaccagca tccgacccac gccgcagccg cgcacatcag gatcgataaa
      601 cagcgcatcc atatgctgcc cacttaacaa cataaaccca accggctgat cccgttcatt
      661 aaccgcgacc cacaacggtg cttccggcag aaaggaacga accagctctt ccagctcggc
      721 ccgatactct gctgataaaa aatcgtgggt ggcatcgaca gatcgacacc aaatcgcaac
      781 gagttcctcc ccttcctcat gctgtgagcg gcgaatacta ataaccattt tctctccttt
      841 tcgtcattct tatattctaa cgtagccttt tccttgaaac tttctcacct tcaacatgca
      901 ggctcgacat tggcaaattt tctggttatc ttcagctatc tggatgtcta aacgtataag
      961 cgtatgtagt gaggtaatca ggttatgcct attcgtgtgc cggacgagct acccgccgtc
     1021 aatttcttgc gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa
     1081 attcgtccac ttaaggttct aatccttaac ctgatgccga agaagattga aactgaaaat
     1141 cagtttctgc gcctgctttc aaactcacct ttgcaggtcg atattcagct gttgcgcatc
     1201 gattcccgtg aatcgcgcaa cacgcccgca gagcatctga acaacttcta ctgtaacttt
     1261 gaagatattc aggaacagaa ctttgacggt ctgattgtaa ccggtgcgcc gctgggcctg
     1321 gtggagttta atgatgtcgc ttactggccg cagatcaaac aggtgctgga gtggtcgaaa
     1381 gatcacgtca cctcgacgct gtttgtctgc tgggcggtac aggccgcgct caatatcctc
     1441 tacggcattc ctaagcaaac tcgcaccgac aaactctctg gcgtttacga gcatcatatt
     1501 ctccatcctc atgcgcttct gacgcgtggc tttgatgatt cattcctggc accacattcg
     1561 cgctatgctg actttccggc agcgttgatt cgtgattaca ccgatctgga aattctggca
     1621 gagacggaag aaggggatgc atatctgttt gccagcaaag ataagcgcat tgcctttgtg
     1681 acgggccatc ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa
     1741 gccggactag gcccggaagt accgtataac tatttcccgc acaatgatcc gcaaaataca
     1801 ccgcgagcga gctggcgtag tcacggtaat ttgctgttta ccaactggct caactattac
     1861 gtctatcaga tcacgccata cgatctacgg cacatgaatc caacgctgga ttaatcttct
     1921 gtgatagtcg atcgttaagc gattcagcac cttacctcag gcaccttcgg gtgccttttt
     1981 tatttccgaa acgcacctca ggaggtgaat aaattttatt catattgtta tcaataagtt
     2041 atcaagtatt tttaattaaa atggaaattg tttttgattt tgcattttaa atgagtagtc
     2101 ttagttgtgc tgaacgaaaa gcgcacaacg atccttcgtt cacagtgggg aagttttcgg
     2161 gtccatgacg aggagctgca cgatgactga acaggcaaca acaaccgacg aactggcttt
     2221 cataaggccg tatggcgagc aggagaagca aattcttact gccgaagcgg tagaatttct
     2281 gactgagctg gtgacgcatt ttacgccaca acgcaataaa cttctggcag cgcgcattca
     2341 gcagcagcaa gatattgata acggaacttt gcctgatttt atttcggaaa cagcttccat
     2401 tcgcgatacg gactggaaaa ttcgtggtat tcccgcggac ttacaagatc gtcgagtcga
     2461 gataactggc ccggttgagc gcaagatggt gatcaacgca ctgaacgcca atgtgaaagt
     2521 ctttatggcc gatttcgaag attcactggc accagactgg aacaaagtga tcgacgggca
     2581 aattaacctg cgcgatgcgg ttaacggcac catcagctat accaatgaag caggcaaaat
     2641 ttatcagctc aagcccaatc cagcggtttt gatttgtcgg gtacgcggtc tgcacttgcc
     2701 ggaaaaacat gtcacctggc gtggtgaggc aatccccggt agcctgtttg attttgcgct
     2761 ctatttcttc cacaactatc aggcactgtt ggcaaagggc agcggtccct atttctatct
     2821 gccgaaaacc cagtcctggc aggaagcggc ctggtggagc gaagtcttca gctatgcaga
     2881 agatcgcttt aatctgccgc gcggcaccat caaggcgacg ttgctgattg aaacgctgcc
     2941 cgccgtgttc cagatggatg aaatccttca cgcgctgcgt gaccatattg ttggtctgaa
     3001 ctgcggtcgt tgggattaca tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg
     3061 cgtcctgcca gacagacagg cagtgacgat ggataaacca ttcctgaatg cttactcacg
     3121 cctgttgatt aaaacctgcc ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt
     3181 tattccgagc aaagatgaag agcgcaataa ccaggtgctc aacaaagtaa aagcggataa
     3241 agcgctggaa gccaataacg gtcacgatgg cacatggatt gctcacccag gccttgcgga
     3301 tacggcaatg gcggtattca acgacattct cggctcccgt aaaaatcagc ttgaagtgat
     3361 gcgcgaacaa gacgcgccga ttactgccga tcagctgctg gcaccttgtg atggtgaacg
     3421 caccgaagaa ggtatgcgcg ccaacattcg cgtggcagtg cagtacatcg aagcatggat
     3481 ctccggcaac ggctgcgtgc cgatttatgg cctgatggaa gatgcggcga cggctgaaat
     3541 ttcccgtacc tcaatctggc agtggatcca tcatcaaaaa acgttgagca atggcaaacc
     3601 ggtgaccaaa gccttgttcc gccagatgct gggtgaagag atgaaagtca ttgccagcga
     3661 actgggcgaa gaacgtttct cccaggggcg ttttgacgat gccgcacgct tgatggaaca
     3721 gatcaccact tccgatgagt taattgattt cctgaccctg ccaggctacc gcctgttagc
     3781 gtaaaccacc acataactat ggagcatctg cacatgaaaa cccgtacaca acaaattgaa
     3841 gaattacaga aagagtggac tcaaccgcgt tgggaaggca ttactcgccc atacagcgcg
     3901 gaagatgtgg tgaaattacg cggttcagtc aatcctgaat gcaccctggc gcaactgggc
     3961 gcggcgaaaa tgtggcgtct gctgcatggc gagtcgaaaa aaggctacat caacagcctc
     4021 ggtgcactga ctggcggtca ggcgctgcaa caggcgaaag cgggtattga agcagtctat
     4081 ctgtcgggat ggcaggtagc ggcggacgct aacctggcgg ccagcatgta tccggatcag
     4141 tcgctctatc cggcaaactc ggtgccagct gtggtggagc ggatcaacaa caccttccgt
     4201 cgtgccgatc agatccaatg gtccgcaggc attgagccgg gcgatccgcg ctatgtcgat
     4261 tacttcctgc cgatcgttgc cgatgcggaa gccggttttg gcggtgtcct gaatgccttt
     4321 gaactgatga aagcgatgat tgaagccggt gcagcggcag ttcacttcga agatcagctg
     4381 gcgtcagtga agaaatgcgg tcacatgggc ggcaaagttt tggtgccaac tcaggaagct
     4441 attcagaaac tggtcgcggc gcgtcttgca gctgacgtga cgggcgttcc aaccctgctg
     4501 gttgctcgta ccgatgccga tgcggcggat ctgattacct ccgattgcga cccgtatgac
     4561 agtgaattta ttactggcga gcgtaccagt gaaggcttct tccgtactca tgcgggcatt
     4621 gagcaagcga tcagccgtgg cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa
     4681 acctccacgc cggatctgga actggcgcgt cgctttgcac aagctatcca cgcgaaatat
     4741 ccgggcaaac tgctggctta taactgctcg ccgtcgttca actggcagaa aaacctcgac
     4801 gacaaaacta ttgccagctt ccagcagcag ctgtcggata tgggctacaa gttccagttc
     4861 atcaccctgg caggtatcca cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat
     4921 gcccagggcg agggtatgaa gcactacgtt gagaaagtgc agcagccgga atttgccgcc
     4981 gcgaaagatg gctatacctt cgtatctcac cagcaggaag tgggtacagg ttacttcgat
     5041 aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac cggctccact
     5101 gaagaatcgc agttctaagc aacaacaacc gttgctgact gtaggccgga taaggcgttc
     5161 acgccgcatc cggcaatcgg tgcacgatgc ctgatgcgac gcttgcgcgt cttatcatgc
     5221 ctacagccgt tgccgaacgt aggccggata aggcatttac gccgcatccg gcaatcggtg
     5281 cacgatgcct gatgcgacgc ttgcgcgtct tatcatgcct acaaccgttg ccgaacgtag
     5341 gctggataag gcgtttacgc cgcatccggc aattctctgc tcctgatgag ggcgctaaat
     5401 gccgcgtggc ctggaattac tgattgctca aaccattttg caaggcttcg acgctcagta
     5461 tggtcgattc ctcgaagtga cttccggggc gcagcagcgt ttcgaacagg ccgactggca
     5521 tgctgtccag caggcgatga aaaaccgtat ccatctttac gatcatcacg ttggtctggt
     5581 cgtggagcaa ctgcgctgca ttactaacgg ccaaagtacg gacgcggcat ttttactgcg
     5641 cgttaaagag cattacacgc ggctgttgcc ggattacccg cgcttcgaga ttgcggagag
     5701 cttttttaac tccgtgtact gtcggttatt tgaccaccgc tcgcttactc ccgagcggct
     5761 ttttatcttt agctctcagc cagagcgccg ctttcgtacc attccccgcc cgctggcgaa
     5821 agactttcac cccgatcacg gctgggaatc tctactgatg cgcgttatca gcgacctgcc
     5881 gctgcgcctg cgctggcaga ataaaagccg tgacattcat tacatcattc gccatctgac
     5941 ggaaacgctg gggacagaca acctcgcgga aagtcattta caggtggcga acgaactgtt
     6001 ttaccgcaat aaagccgcct ggctggtggg caaactgatc acgccttccg gcacgttgcc
     6061 cttcttactg ccgatccacc agacgaacga cggcgagtta tttattgata cctgcctgac
     6121 gacgaccgcc gaagcgagca ttgtttttgg ctttgcgcgt tcttatttta tggtttatgc
     6181 accgctgccc gcagcgctgg tcgagtggct acgggaaatt ctgccgggta aaaccactgc
     6241 tgaattgtat atggctatcg gctgccagaa gcacgccaaa accgaaagct accgcgaata
     6301 tctcgtttat ctacagggct gtaatgagca gttcattgaa gcgccgggta ttcgtggaat
     6361 ggtgatgttg gtatttacgc tgccgggctt tgatcgggta ttcaaagtca tcaaagacaa
     6421 gttcgcgccg cagaaagaga tgtctgccgc tcacgttcgt gcctgctatc aactggtgaa
     6481 agagcacgac cgtgtgggcc gaatggcgga cacccaggag tttgaaaact ttgtgctgga
     6541 gaagcggcat atttccccgg cattaatgga attactgctc caggaagcag cggaaaaaat
     6601 caccgatctt ggtgagcaga ttgtaattcg ccatctctat atcgagcggc ggatggtgcc
     6661 gctcaatatc tggctggaac aagtggaagg tcagcagttg cgcgacgcca ttgaagaata
     6721 cggtaacgct attcgccagc ttgccgctgc taacattttt cctggcgaca tgctgtttaa
     6781 aaacttcggt gtcacccgtc acgggcgtgt ggttttttat gattacgatg aaatttgcta
     6841 catgacggaa gtgaatttcc gcgacatccc gccgccgcgc tatccggaag acgaacttgc
     6901 cagcgaaccg tggtacagcg tctcgccggg cgatgttttc ccggaagagt ttcgccactg
     6961 gctatgcgcc gacccgcgta ttggtccgct gtttgaagag atgcacgccg acctgttccg
     7021 cgctgattac tggcgcgcac tacaaaaccg catacgtgaa gggcatgtgg aagatgttta
     7081 tgcgtatcgg cgcaggcaaa gatttagcgt acggtatggg gagatgcttt tttgagtaaa
     7141 gcttccatat aatttttctc cgcaatgtat cgagggttat ccgtaaagcc aaagctttca
     7201 gccatcttat ttaatgtatt aaggattaat tcagcaataa cccggtgacc caattcaaaa
     7261 gccaactcaa aggcagagta tttttgtggg gctttgtgtt gccaaaaatc cataatatct
     7321 tcagcggtaa atccaaacag gcgtgcatgg tta
//
LOCUS       DAZGTR010000182         7317 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGTR010000182 DAZGTR010000000
VERSION     DAZGTR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.184"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(298..1434)
                     /locus_tag="ACHLCY_004509"
     CDS             complement(298..1434)
                     /locus_tag="ACHLCY_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0371610.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYITQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1437..1799)
                     /locus_tag="ACHLCY_004510"
     CDS             complement(1437..1799)
                     /locus_tag="ACHLCY_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0371611.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2336..4249
                     /gene="mtlA"
                     /locus_tag="ACHLCY_004511"
     CDS             2336..4249
                     /gene="mtlA"
                     /locus_tag="ACHLCY_004511"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP0371612.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4479..5627
                     /gene="mtlD"
                     /locus_tag="ACHLCY_004512"
     CDS             4479..5627
                     /gene="mtlD"
                     /locus_tag="ACHLCY_004512"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0371613.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5627..6214
                     /gene="mtlR"
                     /locus_tag="ACHLCY_004513"
     CDS             5627..6214
                     /gene="mtlR"
                     /locus_tag="ACHLCY_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP0371614.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6226..6435)
                     /gene="yibT"
                     /locus_tag="ACHLCY_004514"
     CDS             complement(6226..6435)
                     /gene="yibT"
                     /locus_tag="ACHLCY_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP0371615.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6720..7082
                     /locus_tag="ACHLCY_004515"
     CDS             6720..7082
                     /locus_tag="ACHLCY_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP0371616.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1867 a         1840 c         1845 g         1765 t
ORIGIN      
        1 gtgactgtca tccttcagat aataaagcca tcatcgcaat agatggaaaa gcactcctgc
       61 tcttttttga ttagagccgt cgcaggagga acaactcata tcatcattgc gatctcgacg
      121 atgtatagtc tcatcctgcg gcagaacaag acggataaaa aatccaatga gagcacactc
      181 atccagaact tattaacata atgaatatta aagaaaaatc atcgcagcta atgaagtaat
      241 atgcaaaaat aattagtcct tacaaggagc aaagttccat aatctccccc ctccccctca
      301 atgatccaaa taaagataat gcatccagct ggtcattctt agcagcactt tccgcatcac
      361 cgaaacatgg ctgaaatggt cggagtaaac cgccacctgg gcgtagatgc cgtcgggtaa
      421 ggcatcgata tcatcgttag ggtccagttc aatggttccc agcacaccgt ccgtgccggg
      481 cacgaccgtt aatgattgca ataccccctg cgcctgataa gaaccgcctg gcacgacagg
      541 taaaatactg gtcagtttgc cgtggaacac ctgcccaggt agcgcgttaa acaccacttc
      601 tgcatcatca ccaggtttca gacgtaacag cgagttttgc cgaaattggg cgacaatttg
      661 ccgtttttgc tcggggatga aaaccatcac cggacgcaac ggcaaggcag ctgcgtatgt
      721 acctgggcgg atcagtacct gagtaatgta gccattgctc ggcgcgcgaa tgacagtctg
      781 ctcaagatta tattttgctt cagtaagttg cgctcttaag ctcacaatct gcgattgctc
      841 gccgttaacc atactgtcga gctggctctg gatctgcgcc tgctccgcca ccgagccttt
      901 caccagcgca tcctgcgcga ggaaattttg ccgcgcatcg tcgatgtcac gttccgagaa
      961 cggattcacc gccgcctggc tgcctttcag ataacgttga taatttttaa acagacggtc
     1021 gcgctccgct gaaacctggg tggtgttggc ctgcgcttct gtgagctgcg cgcgcagagt
     1081 ctttatatta tgcgtcgccg tcatcaggtc agcctgaagc ctgtcaactc gcgcctggta
     1141 acgaaccggg tcgagcttaa aaagcacctc gcccttttga ataagctgat tattcttgtc
     1201 agtgacttca gtaacaattc ccgtcacctg tggcgtgatg gggatcgcta tcactgcctt
     1261 ttgcgcggta aaagtgtaag ggtggttgta gttcatcaac aaaatcaaac cactcaccag
     1321 aaacacgcca cccagcgccg ccgtcgccag cgtccactga ttcaccggaa tacgaaagat
     1381 tttaaagacc gcccacgcca gcgccacgta agttaaaaca atcaatagat ccataattag
     1441 atctccggga acgtggaata gtcagtcttt ttctcggcgg cgagttgatg ctccagccgg
     1501 gaaattcggt cagaaagtgc ggcgatttcc gggtcagtcg ctttctcctg cgacgcaaca
     1561 tgtgactgca taccccagcc acgctccggt tgatagagcg tcgcccagat ccacagaaac
     1621 ggccagataa catgcagagt aaacaggctc acccagcccg ccgtatgaat agcgtcggca
     1681 tggggatggt tgcgcttttt ggcaatcaga taagggatgt catgtatcgc gatgattcca
     1741 taaaaaatca ccagaaagac gaagatcagc actcccaacg cgaaatagtt tagaaacata
     1801 tctgcctcgg attcacgttt atcagtgttg tttttgggct ggcagccaga agggagtcag
     1861 gctgatattt tgacaataat ccgggttcgc gattctcgcc ataacaccaa agaataattt
     1921 ttagaggtga tgagttgctt agttacataa cgattgtatg acgaaggcat aacatgctgt
     1981 agatcacatc aggtgaacgc cgtaagaaaa tatcttgtga ttcagatcac aaagattcaa
     2041 caaaccatca aaacaaaaat gtgacactac tcacatttaa atgccatttt tagcgaaaat
     2101 cgccgccttg ttgctttttt acacaagcgt tttgtgatga acatcacgtc aattacctct
     2161 ctacccccta tatttatgtg attgatatca cacaaaaggc cgtcgactgg acagttaacc
     2221 gattcagtgc cagatttcgc agtatctaca aggtccggct acctctgccg ccacattaac
     2281 aaaaaacctc gggcttccag cctgcgcgac agcaaacata agaaggggtg tttttatgtc
     2341 atccgatatt aagatcaaag tgcaaagctt tggtcgtttc ctcagcaaca tggtgatgcc
     2401 aaatatcggc gcgtttatcg cgtggggtat catcaccgcg ttatttattc caacagggtg
     2461 gttaccgaac gagacgctgg cgaagctggt cgggccgatg atcacttatc tcctgccgct
     2521 gctgatcggt tataccggtg gtaagctggt aggcggcgaa cgtggcggcg tagtcggtgc
     2581 catcaccacc atgggcgtta tcgtcggcgc agacatgccg atgttcctcg gttctatgat
     2641 tgcaggtccg ctgggcggct ggtgcattaa gcacttcgac cgctgggtag acggtaagat
     2701 caaatccggt tttgagatgc tggtgaataa cttctccgca ggcatcatcg ggatgatcct
     2761 cgctattctg gcattcctcg gcattggccc gattgttgaa gccctgtcca aaatgctggc
     2821 tgcgggcgtt aacttcatgg ttgtccatga catgctgccg ctggcgtcta tctttgttga
     2881 accggcgaaa atcctgttcc tcaacaatgc cattaaccac ggtatcttct cgccgctggg
     2941 tattcagcag tcccatgaac tgggtaaatc aatcttcttc ctgattgaag ctaacccagg
     3001 tccaggtatg ggcgtgctgc tggcgtacat gttctttggt cgtggtagcg ctaaacagtc
     3061 tgcgggcggt gcggcaatca tccacttcct gggtggtatc cacgaaatct acttcccgta
     3121 tgtgctgatg aatccgcgtc tgatcctcgc agtcatcctc ggcggtatga ctggcgtgtt
     3181 cacgctgact atcctgggcg gtggtctggt ttctccggca tctccgggtt ctatccttgc
     3241 tgtactggcg atgacaccaa aaggtgctta tttcgctaac atcgcgggtg tgtgtgcggc
     3301 gatggctgtc tccttcgttg tctctgctat tttgctgaaa accagcaaag tgaaagaaga
     3361 agatgatatt gaagcagcaa ctcgtcgtat gcaggacatg aaagctgagt ctaaaggcac
     3421 atctccgctg tctgcgggcg atgtgactaa cgacctgagc cacgtacgta aaatcatcgt
     3481 tgcctgtgac gccggtatgg gttcaagtgc gatgggcgca ggcgtgctgc gtaagaaaat
     3541 tcaggatgca ggtctgtcac agatttcggt cactaacagc gcgatcaaca acctgccgcc
     3601 agatgtggac ctcgtcatca ctcaccgtga cctgaccgaa cgcgctatgc gccaggttcc
     3661 gcaggcgcag catatttcgc tgaccaactt cctcgacagc ggcctgtaca ccagcctaac
     3721 cgaacgtctc gttgctgccc agcgccatac tgaaaacgaa gtgaaagtga aagacagtct
     3781 gaaagacagc tttgacgatt ccagtgctaa cctgttcaaa ctgggcgcgg agaacatctt
     3841 cctcggtcgc aaagcagcaa ccaaagaaga agcgattcgt tttgctggcg agcagctggt
     3901 gaaaggcggt tacgttgagc cggaatacgt tcaggcgatg ctggatcgtg aaaaactgac
     3961 cccgacttat ctgggtgagt ctatcgcggt gccacacggt acggttgaag cgaaagatcg
     4021 cgtactgaaa acgggcgtgg tgttctgcca gtacccggaa ggcgtgcgct tcggtgaaga
     4081 agaagatgac attgcccgtc tggtgattgg tattgcagcc cgtaacaacg agcacattca
     4141 ggttatcacc agcctgacca atgcactgga tgatgagtcc gtcatcgagc gtctggcaca
     4201 caccaccagc gtggatgaag tgctggaact gctggcaggt cgtaagtaat ccaatcccac
     4261 cctctccaca tggagaaggt ggggttaatt gcctgatgcg ctacgcttat caggcctaca
     4321 ggatgcatca caatttgttg aatttgcacg ttcttgtagg ccggataagg cgcttacgcc
     4381 gcatccggcg ctgcccctct cctcacggag agggtttggg tgagggaaaa gcctcacccc
     4441 agccctctcg ggtaaaaaca ttgatgaagg ttaatactat gaaagcatta cattttggcg
     4501 caggtaatat cggtcgtggc tttatcggta aactgctggc agacgcgggt atccaactga
     4561 cgtttgccga tgtcaatcag gtggtacttg atgccctgaa tgcccgtcat agctatcagg
     4621 ttcatgtggt tggtgaaacc gagcaggtag ataccgtttc cggcgtcaat gctgtcagca
     4681 gcattggtga tgatgtcgtt gatctgattg ctcaggtcga tttagtcact accgccgttg
     4741 gcccggttgt gctggaacgt attgctccgg ctatcgccaa agggctggtg aaacgcaaag
     4801 aacaaggtaa tgaatccccg ctgaacatca tcgcctgtga aaacatggta cgcggtacca
     4861 cgcagctgaa aggccatgtg atgaacgccc tgccggaaga cgccaaagcg tgggtagaag
     4921 aacacgttgg ctttgtcgat tccgccgttg accgcatcgt accgccttcg gcttcggcaa
     4981 ctaacgatcc gctggaagtg acggtagaaa ctttcagcga atggattgtc gataaaacgc
     5041 agttcaaagg cgcactgccg aacatcccag gcatggagtt aaccgacaac ctgatggcat
     5101 ttgtcgaacg taaactcttc accctgaaca cgggtcatgc tataaccgcg tacctcggaa
     5161 aactggccgg tcatcagacc attcgtgacg ctattctcga cgagaaaatc cgcgcggtgg
     5221 taaaaggtgc gatggaagaa agtggtgcgg tactgatcaa gcgctacggc tttgacgcag
     5281 acaaacatgc ggcgtacatc cagaaaatcc tcggtcgttt tgagaacccg tatctgaaag
     5341 atgatgtaga gcgcgtaggc cgtcagccgc tgcgtaaact gagtgctggc gaccgtctga
     5401 tcaagccact gctcggtacg ctggaataca gccttccgca caagaatctg attcagggga
     5461 ttgctggtgc aatgcacttc cgcagtgaag atgatccgca ggctcaggaa ctggcagcac
     5521 tgatcgctga caaaggtccg caggcggcgc tggcacagat ttccggtctt gatgccaaca
     5581 gcgaggttgt atccgaggcg gtaaccgctt ataaagcaat gcaataatgg tggaccaggc
     5641 gcaggacacc ctgcgcccga ataacagatt gtcagatatg caggcaacaa tggaacaaac
     5701 ccaggccttt gaaaaccgtg tgcttgagcg tctgaatgct ggcaaaaccg tgcgaagctt
     5761 tctgatcacc gccgtcgagc ttttgaccga ggcggtaaat cttctggtgc ttcaggtatt
     5821 ccgcaaagac gattacgcgg tgaagtatgc tgtagaaccg ttactcgacg gcgacggtcc
     5881 gctgggcgat ctttctgtgc gtttaaaact catttacggg ttgggcgtca ttaaccgcca
     5941 ggaatacgaa gatgcggagc tgctgatggc attgcgtgaa gaactaaatc acgacggcaa
     6001 cgagtacgcc tttaccgacg acgagatcct tggacccttt ggtgaactgc actgcgtggc
     6061 ggcgttacca ccgccgccac agtttgaacc agcagactcc agtttgtatg caatgcaaat
     6121 tcagcgctat caacaggctg tgcgatcaac aatggtcctt tcactgactg agctgatttc
     6181 cagaatcagc ttaaaaaaag cctttcaaaa gtaagcaacg tctgcttact gcccctctac
     6241 ctgcttcggc cgataaagcc gacgataata ctccagacgt tgtagatata acggcacgct
     6301 ttcatcgggt attccggacg gaatcgcgtt acggggagga agttttttca gatactcccg
     6361 gaacgcctgg cttgatgcca tgaaatctac ggctttatcg ataagaagcg gaacgttttc
     6421 acctaatttg cccatgatga tatctccgta ttaccccgcg ccgggaatgc gcggccgcca
     6481 attttagttt aggttcccgg taaagatgac attaaggaaa acgtgctgaa tcctcaataa
     6541 tcatccgctg ctgtttatta tcttttttct atagatttct taattaatca acgaattgcg
     6601 tagtttttga gcatgttaat gattacgttg cagaactatt acaatgtcat cagttgtgaa
     6661 caaagcacct ggtcgcgcat actaggggct ataaatttat ctttatcaga agccatcaca
     6721 tgaaagaagt cgaaaaaaac gaaatcaaac gtctcagcga tcgcctggac gccatccgcc
     6781 accagcaggc cgatctgtcg ctggttgaag ccgcagacaa atatgccgag ctggaaaaag
     6841 agaaagccac gctggaagca gaaattgctc gcctgcgtga ggttcatagc caaaaactga
     6901 gtaaagaagc acagaagctg atgaagatgc cgtttcagcg cgcaattacc aaaaaagagc
     6961 aggctgatat gggcaagctg aagaaaagtg ttcgcggact ggtggttgtg cacccaatga
     7021 ccgcgctggg ccgtgaaatg ggcctgcagg agatgactgg attctctaag actgcgtttt
     7081 aagcgcctca gacgactggt tcgatcatat caacccgcat ctggcttgcc ttagctggtc
     7141 aatgcgggga ctctcctgtc tactcgccta attaccaaac aattcactcc ctttacgtcg
     7201 atttaccgct ggttgatatt ctgaaaatgc atttttaccg aaatagccac ctataaacaa
     7261 taaaaatata tagaagatat catttattaa ttaacaagca tttacgtctc ttttttt
//
LOCUS       DAZGTR010000183         7312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGTR010000183 DAZGTR010000000
VERSION     DAZGTR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.185"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(75..494)
                     /locus_tag="ACHLCY_004516"
     CDS             complement(75..494)
                     /locus_tag="ACHLCY_004516"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP0371617.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(515..1897)
                     /gene="atpD"
                     /locus_tag="ACHLCY_004517"
     CDS             complement(515..1897)
                     /gene="atpD"
                     /locus_tag="ACHLCY_004517"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP0371618.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(1924..2787)
                     /gene="atpG"
                     /locus_tag="ACHLCY_004518"
     CDS             complement(1924..2787)
                     /gene="atpG"
                     /locus_tag="ACHLCY_004518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP0371619.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(2838..4379)
                     /gene="atpA"
                     /locus_tag="ACHLCY_004519"
     CDS             complement(2838..4379)
                     /gene="atpA"
                     /locus_tag="ACHLCY_004519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP0371620.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(4392..4925)
                     /gene="atpH"
                     /locus_tag="ACHLCY_004520"
     CDS             complement(4392..4925)
                     /gene="atpH"
                     /locus_tag="ACHLCY_004520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP0371621.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(4940..5410)
                     /gene="atpF"
                     /locus_tag="ACHLCY_004521"
     CDS             complement(4940..5410)
                     /gene="atpF"
                     /locus_tag="ACHLCY_004521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP0371622.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(5472..5711)
                     /gene="atpE"
                     /locus_tag="ACHLCY_004522"
     CDS             complement(5472..5711)
                     /gene="atpE"
                     /locus_tag="ACHLCY_004522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP0371623.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(5758..6573)
                     /gene="atpB"
                     /locus_tag="ACHLCY_004523"
     CDS             complement(5758..6573)
                     /gene="atpB"
                     /locus_tag="ACHLCY_004523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP0371624.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(6582..6962)
                     /gene="atpI"
                     /locus_tag="ACHLCY_004524"
     CDS             complement(6582..6962)
                     /gene="atpI"
                     /locus_tag="ACHLCY_004524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP0371625.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         1753 a         2002 c         1822 g         1735 t
ORIGIN      
        1 ctcttcagga cggatcacgc gtaaaaaaaa gccagcctgt ttccagactg gcttttgtgc
       61 ttttcaagcc ggtgttacat cgcttttttg gtcaactcga taacgcgcag ctgcgcgatc
      121 gctttggcca gttccgcaga cgcctgagcg taatctacgt cgccgtgaga gctgctaatg
      181 tgctcttcag ccttacgttt cgcttccatg gctcgcgctt cgtcgagatc ctggccgcga
      241 attgcggtgt cggccagaac ggtcacgttg ccaggctgca cttcaagaat gccgccagac
      301 agatagataa actcttcgtg accgtgctgt ttcacgatgc gaatcatacc aggcttaatg
      361 gcggtgagca gcggtgcgtg gccagggtag atccccagtt caccttcgct acccgttacc
      421 tggattttct cgaccagacc agagaacatt tgttgctctg cgctgacgac gtccaggtgg
      481 taagtcattg ccatatcacc ctccgattaa ggcgttaaag ttttttggct ttttccacag
      541 cttcttcgat ggaaccgacc atgtagaacg cctgctccgg caggtgatcg tattcgcctt
      601 ccatgatgcc tttaaagcca cggatggtgt ctttcaggga gacgtattta cccggagaac
      661 cggtgaatac ttctgccacg aagaacggct gggacaggaa gcgctggatc ttacgagcac
      721 gcgctaccac cagtttgtct tcttcagaca gttcatccat acccaggatg gcgatgatgt
      781 ctttcagttc ctgataacgt tgcaggatgg actgaacgcc acgcgcggtg tcgtagtgtt
      841 cctgaccaac caccagcggg tccagctgac ggctggtgga gtccagcggg tcaacggccg
      901 ggtagatacc cagagacgcg atctgacggc tcagtaccac ggttgcgtca aggtgcgcaa
      961 aggtggttgc cggagacggg tcagtcaagt catccgcagg tacgtatacc gcctgtacgg
     1021 aggtgataga accagttttg gtggaggtga tacgttcctg cagaacgccc atctcttccg
     1081 ccagggtcgg ctgataacct accgctgaag gcatacggcc cagcagtgcg gatacttccg
     1141 taccggccag ggtgtaacga tagatgttgt caacgaacag cagaacgtca cgaccttcgt
     1201 cacggaattt ctcagccatg gtcagaccgg tcagagcaac gcgcagacgg tttcccggcg
     1261 gctcgttcat ctggccatac accagggata ctttgtcgat aacgttggag tcggtcattt
     1321 cgtggtagaa gtcgttaccc tcacgagtac gttcacctac gcccgcaaac acagagtaac
     1381 cggagtgctc gatcgcgatg ttacgaatga gctccatcat gtttacggtt ttacctacac
     1441 ccgcaccacc gaacagacca actttaccgc ccttagcgaa cggacacatc aggtcgataa
     1501 ctttgatacc ggtttccagc agttcctgag agtttgacag ctcttcgtag gaaggtgctg
     1561 cgcggtgaat cgcccaacgc tcttcttcac cgatctcgcc tttcatgtcg accggttcac
     1621 ccagtacgtt catgatacgg cccagagtcg ctttacctac cgggacttcg atcgggtgtt
     1681 cgaggtcttt tacatccaga ccgcgacgca gaccgtcgga ggaacccatt gcgatggtac
     1741 gcacgatacc gccgccgagc tgctgctgaa cttccagcac cagacgctca ttaccatttt
     1801 gcacctcaag agcatcgtac acgcgcggta cggcatcctg agggaattcg acgtcaacta
     1861 cggcgccgat tacctggaca atctttccag tagccatctt aaatcctcta cgaaataacc
     1921 tgtttaaacc gcggcggccc ccgagacgat ctcggtgagt tcctgagtaa tgctggcctg
     1981 acgagctttg ttgtatacca actgcagctc tttaatcagg ctgccgccat tgtcggtcgc
     2041 ggctttcatc gccaccatac gggcggcctg ctcgctggcc aggttttcaa ccacgccctg
     2101 ataaacctga gattcgacat aacgacgcag cagggtatcc agcaacgcct tcggatcggg
     2161 ttcgtacagg taatcccagg atttatgttt cagatcatca tcatctgatg ccggtaacgg
     2221 cagcagctgg ctgatggtcg gaacctgaga catggtgtta ataaatttgt tgctgacaat
     2281 gtaaagcttg tccagacggc cttcgtcgta ggcctgcaac atcactttta ccggaccgat
     2341 cagttcggac agggaagggt tatcccccat gccggtgacc tgggcaacaa cattgccgcc
     2401 cacggagttg aagaacgaca cgcctttcga gccgatcatt gcgaggtcgc attgaacgcc
     2461 tttgtcggtc caggtcttca tttccgccag cagttttttg aacaggttaa tgttcaaacc
     2521 accgcacaaa ccacggtcgg tcgacaccac caggtagccc acgcgtttaa cgtcgcgttc
     2581 ttccaggtat gggtgcttat attccagatt accgctcgca aggtgaccaa tcactttgcg
     2641 catggtatct gcataaggac ggctggccgc catgcgatcc tgcgatttac gcattttgga
     2701 agcggcgacc atctccatcg ctttagtgat cttttgcgtg ttctggacgc ttgcgatctt
     2761 actacgtatc tcttttgcgc cggccatgag cttctcctca atgccttgcg gcctgcccta
     2821 aggcaagccg ccagacgtta ccaggattgg gttgctttga aggaatcgag gatgcctttc
     2881 agcttgcctt cgatttcgtc gttgtagcca ccggtctggt tgatctcttg catcaacgga
     2941 gcgtgatcac ggtcgacgta agccagcaga gcggcttcga agctgccgat tttcgacagt
     3001 tcaacatccg ccaggtaacc acgttctgct gcgaacagaa ccagagactg ttgcgctacg
     3061 gacatcggcg catactgttt ctgtttcagc agttcggtca ctttctgacc gtggtcaagc
     3121 tgcttacgtg ttgcatcgtc aaggtcggat gcaaactgag agaacgctgc cagttcacga
     3181 tactgtgcca gagcggtacg gataccaccg gacagttttt tcatgatctt ggtctgtgct
     3241 gcaccaccaa cacgggatac ggaaataccc gggttaaccg caggacgaat accggcgttg
     3301 aacaggttgg tttccaggaa gatctgacca tcggtaatgg agattacgtt ggtcggaacg
     3361 aacgcagaaa cgtcacccgc ctgagtttca ataatcggca gtgcggtcag agaaccggtt
     3421 ttccctttca cttcaccttt ggtgaaggct tcaacgtatt cggcgttaac acgcgcagca
     3481 cgctccagca gacgagagtg gaggtagaat acgtcgcccg ggaatgcttc acgtcctggc
     3541 ggacgacgga gcagcaggga gatctgacgg taagcaacag cctgtttaga caggtcatcg
     3601 taaatgatca gcgcatcttc accgcggtca cggaagtatt cgcccattgc gcaaccggca
     3661 tacggagcca gatattgcag tgcagcggat tcagacgcgg ttgctaccac aacgatggtg
     3721 ttagccagtg cgccgtgctc ttccagttta cgtaccacgt tagaaatggt ggacgctttc
     3781 tggccgatag cgacatagat acatttgata ccggaatcgc gctggttgat gatggcatcg
     3841 atagccagtg cggttttacc ggtctgacgg tcaccgatga tcaattcacg ctgaccacga
     3901 ccaattggga tcatggagtc aacggcttta taaccggtct gtaccggctg atctacggac
     3961 tgacgttcga taacgcccgg agcgattgct tctacagcag agaagccgtc gtgatccagc
     4021 ggacctttac cgtcgattgg tgcacccaga gtgttaacca cacggcccag caggccacgg
     4081 ccaaccggaa cttccaggat acggccagta cacttaactt tcatgccttc ggcaaggtca
     4141 gcgtacggac ccataacaac cgcacctaca gagtcgcgct cgaggttcag tgcgatagcg
     4201 taacggttac ccggcaggga gatcatttca ccctgcatac aatcggccag gccgtgaatg
     4261 cggataacac cgtcacttac agaaacaata gtaccttcgt tgtgagcttc actcacaaca
     4321 ttgaactgag caatgcgctg cttgatcagt tcgctgattt cggtggaatt cagttgcatg
     4381 ctccagtccc cttaagactg caagacgtct gcaaggcgct caagacgacc gcgtacgctg
     4441 ccatcaatga ccatatcacc cgctcggatg ataacgcctg ccattacaga cttatcgatt
     4501 ttgcaattca gcttaacttt gcgtgacaga cgtttttcca tcgcagcaga aattttcgcg
     4561 agctgttgtt cactcagtgc ggcagcggaa atgacgtcta cctcagcggt agcctcactc
     4621 acggcacgaa ggtgaataaa ctgctccaga acatccggga gcgcgttaag acgaccattt
     4681 tcagccatta cccgaatcag gttctgaccg ttttcgtcca gttgctcacc acaaactgcg
     4741 ataaacgact cggcgagcgt ttctggcgca agcgcgccag agagaagctc tgccatttgt
     4801 tcgtttttgg ttacctcggc ggcaaacgcc agcatgtcct gccagcgttc tacactttgg
     4861 tgttcgacgg caaagtcaaa agctgctttg gcgtaggggc gagctaccgt aataaattca
     4921 gacatcagcc cctccctcct tacagttcag cgacaagttt atccacgatg tcgctgttag
     4981 cagcttcatc cacggaacgt tcgatgatct tctcggcgcc agcaacagcc aggatagcaa
     5041 cttgcttacg cagctcttca cgggcacgtt tacgctcggc ttcaatttct gcctgcgcct
     5101 gggccacgat tttagtacgt tcctgttctg cctcagcttt cgcttcgtcc agaatctgcg
     5161 agcggcgttt gttcgcctgc tcgatgatta cctgggcttc cgctttcgct tttttcagct
     5221 ggtcggtcgc gctggccttt gcaaggtcaa ggtccttatg tgctcgttct gcggaagcaa
     5281 ggccgtcagc aatttctttt tgacgttttt cgatggctgc cattaatggc ggccatacgt
     5341 acttcatgca gaacagaacg aacaggacaa acgcgatggc ctggccgagg attgttgcgt
     5401 taagattcac agcacaatgc ctctatttag ttaacgttct gatattgctc tttaaataaa
     5461 agcaacgctt actacgcgac agcgaacatc acgtacagac ccagacctac agcgatcatc
     5521 gggatagcat ccaccagacc cataacgata aagaactgag tacgcagcag aggaatcaga
     5581 tcaggttgac gcgctgcgcc ttccaggaat ttacccccga ggatgccgat accgatcgca
     5641 gcaccgattg ccgccagacc catcatcaca gcggcagcca tgtacagcag atccatattc
     5701 aggttttcca tgacagtctc cagtttgttt cagttaaaac gtagtagtgt tggtaaatta
     5761 atgttcttca gacgccatcg acagatagac gatcgtcaga accatgaaga tgaaggcttg
     5821 cagcgtaatg atcaggatgt ggaaaatggc ccacggcaca ttcaggatcc actgtgacca
     5881 ccacggcaac agaccagcaa tcagaatgaa aatcagctca ccggcataca tgttaccgaa
     5941 cagtcgcaaa ccaagtgaaa ctggtttgga cagcaggctt accccttcaa ggattaagtt
     6001 gacaggaatg aacgcccagt gattgaacgg ctgcagcgtc aactctttcg tgaagccgcc
     6061 gatgcctttc attttgatgc tgtagaacag aatcaggata aatacgccca atgccataga
     6121 cagcgttacg ttcacgtccg cagacggaac cacacgcagt gcaggcagac ccagtacatg
     6181 ttcagcaatg tacggcagca ggtcgatagg cagtaaatcc atcaggttca tcaggaatac
     6241 ccagacgaag atcgtcaggg ccagcggagc aatcagcttg cttttgccat ggtacatgtc
     6301 tttcacgcta ccattaacaa agccgatcac cagctcaatc gcggtctgaa acttacctgg
     6361 cacaccgctg gtcgcctttt tggctacgct acggaataaa accaggaaca acagacccag
     6421 caccaccgag aagaacatgg agtcaatatt gattgtccag aaggtggctg gggggttttg
     6481 tggatccacc agcgagaatg tacgcaggtc cagctgaagg ttattcaggt ggtgtcctat
     6541 gtaatcctgc ggcgtcatat tttctgaagc catgatgcct tttacccttt gttgttaatt
     6601 acagccggtg ccagtatctg aaccaccagc accaaaaccc acgtaacgat cagcggcaag
     6661 aataccgcct ttaaaaccgc caacgccacc accagtaaca ccaacatcgc cagaactttg
     6721 aaagcttcgc caaatgcgaa tgtccaggcc acccggcctt tcgctggtgt atgcgcctgg
     6781 tgacgccagg caaatatcat aaacaaaacg ttaggcagaa agactgccag gcccccgctt
     6841 attgcagaga cgccccagaa ggggtctttg aggctgaaca gcaatccact tgctatcacc
     6901 accagtaact gaacgagcag aagcttccga gcaacgtttc gactcacgag cgacacagac
     6961 atcacgtttt tcactcctgc tcccttcgag gtatgccgcg tgtcgtataa aactttcttt
     7021 aaggcttaga gtcaagcatc aaaaagcggt caaattatac ggtgcgcccc cgtgatttca
     7081 aacaataagt agccaaaagg tgaataaatg tttaaatatt tttccagtgc atacaattgc
     7141 gacttttctg ctaaccctgt tcgatcatga aaaactgtaa ataacgcgta aacactggtg
     7201 ataaagcgtg cttcagatca catattgcgc atgttcgcgc acagcatatt tatttacttg
     7261 gcaaatgatg cctttgcaag tttatgatat ttcagtctaa aaacagatac tg
//
LOCUS       DAZGTR010000184         7217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGTR010000184 DAZGTR010000000
VERSION     DAZGTR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.186"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            149..885
                     /locus_tag="ACHLCY_004525"
                     /pseudo
     CDS             149..885
                     /locus_tag="ACHLCY_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1064..1360
                     /locus_tag="ACHLCY_004526"
     CDS             1064..1360
                     /locus_tag="ACHLCY_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0371626.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1668..1784
                     /locus_tag="ACHLCY_004527"
     CDS             1668..1784
                     /locus_tag="ACHLCY_004527"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371627.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            2003..3436
                     /locus_tag="ACHLCY_004528"
     CDS             2003..3436
                     /locus_tag="ACHLCY_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0371628.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQTPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
     gene            4257..4820
                     /locus_tag="ACHLCY_004529"
     CDS             4257..4820
                     /locus_tag="ACHLCY_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0371629.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            4975..>7217
                     /locus_tag="ACHLCY_004530"
     CDS             4975..>7217
                     /locus_tag="ACHLCY_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0371630.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVR"
BASE COUNT         2034 a         1420 c         1723 g         2040 t
ORIGIN      
        1 ttgcttttct gttaactggt tttcattttt gaatcaatat tttctgaaca gggtaatagt
       61 acacgtatta acagattatg tcagaaggcc ccggtgatgc cttctgacga tagattaatg
      121 ttgtattcta ttggtatctg gagaccatat ggcaacagtt acagtatatt ctccccgctg
      181 taattcctat aaagtgtacc gttatgatca cagcacctca gatcatgagc gtttccagtg
      241 ctaatcctgc aaacgtgtat ttcaactgaa ttacatctac gaaacccgaa aaccaggcat
      301 taaagagcaa attgttgaaa tggttcataa cggagccggg gttcgcgata gctccagaac
      361 gctgaaggtg gatatcaaca ccgtcattct cactttaaaa aacgctcacc acgtaaagta
      421 accatatctc cagccgccag tgtcgatgtg gcacttatct gtgaacttga tgaacaatgg
      481 agttttgtcg aaaacaaagc tcgtcagcag tggcactggt acgcgtataa gaccaaagct
      541 gacggtgtgc tggcttacac ttttggtcct cgcactgatg aaacatgccg tgagttgccg
      601 gaattcctga agctattctc cgcaggcatg ataaccagag acaaccggag cagttatacc
      661 agagaaatgc cacaggataa gcatcttgtc ggcaagatat ttacccggcg cattgagcgc
      721 aacaatctga cacttcgtac tcacatcaaa cgacctgccc ggaaaacgat ttgcttttta
      781 cgctccctgg aaattcacga aaagccattg gttcatttat cgaaaacaca tgtactactg
      841 acaggagtca ttaccagggc ctcttttgct gtttttttgc catgaatgat aatgagaatc
      901 gacagcgata agggaaaagt aaaataacac taatgctgtc gttttctatc atattgattt
      961 ttaattattt attgtctttt ttctcaaccc atttttttac gcagacaaat ttacccgggg
     1021 aatacaaact gtaacgacag ttcaccacac tatgggggat aaaatgccag atcaatccgg
     1081 acactgcagg actccttcac gcatcttacg gatgcctgaa ctgagccaat tgttaggtat
     1141 atctcgttcg acaatttacg aaaaaatgaa tccgttatcg aaatactacg atgcaacctt
     1201 ccccaggccg gtacgtcttg gtagtggttc tgtgggctgg cgttcatcag ctattgacga
     1261 atggcttact ttacatactg tacctgccag atcagcagtc aaaggtgtta atgacgaagt
     1321 gagacatacg ctttcttcag atatgaccat gagcagataa gtttttgtca tcacgtgatc
     1381 acctcaggag gaaaaagaag cataagaaca atttagccag aagtttaatt gataaacaga
     1441 atttccagca ttcattgttg aaagagcagc tattgtcgct attgtagcgg taattgctgc
     1501 tataaccgaa acgtattacg aaaaaggaag catttatgcc ttacatcaaa ccggatatgt
     1561 cgtattcagt atatgcatga ccatcaatga tagggaaggc aataattaaa gctcaataca
     1621 ataaacaagt acctctgact ctggttgcaa tgtcagtatt aacagcgatg tcaattgcct
     1681 gtcagaatca ggttgacgta tgcagtcccg gaaatttacg tggggccgtt aatatttaca
     1741 ctatggttct ggagaaaggg aaaagaccgg gggaaaggta ttagtgaaat aatttccgga
     1801 ggtattaaga atgagaaata tgaatgatat ttcgtggtga ataatacagt cttcactgaa
     1861 ttaaccttat cggtttatta aacatcagat ctacaaatgc tctgtatttc tcagagcatc
     1921 aggtctgaat agcagctatt ttcgctattg tagcgggatt tgctgctatt ttactaaccc
     1981 attaatgaaa agagaggctt ttatgtgctt acttgcacca gaaaatcctt atccgatata
     2041 tgcactacca ccgctggtga gaaatgcaat aattgaaact caaaaaaata cacagacacc
     2101 tttggctatg gtggcaacat ccgcattaac agcgatctca attgcctgtc agaatcagat
     2161 tgacgtgtgc agacctggaa atttacatgg gcctgttaat ctttactctc tgattctggc
     2221 tgattccggt gaaaggaaaa cgaccgtgga taaagtgttt atgaaagcat tttatctcag
     2281 ggatgaagcc ctggcggatg aatacgcgaa actggttgag aattacagta cagaaaagga
     2341 aatatgggag caaaaacaaa aagcgctgga atcaaaattt cataaagaga ttcgtgccgg
     2401 taaagattac aaggcaacag aatcagagct tgaaacgcat ctgaataaac ctcctgttcc
     2461 gccgcagata cgtcgaacaa tttttaatga gacaacgata gagggaatgt taaaatatta
     2521 ctccgatagc aatcgctctt ttgctcttgt atccagtgaa gggggggtaa tttttgacag
     2581 ccgggccatg agtaaactgg gaattattaa cactttgtgg gatggaggtt ctcttttcat
     2641 cgacaggaaa tcatctcccg gaattaattt gaaggagcca agactgacga tatcggtgat
     2701 gattcagcct gatgtttacc acaaaggttt ttgtacgcga aaaaaagaaa ttgtgaaaac
     2761 gtcaggacat catgcaaggt ttttgatgtg tcaaccaaca tcaacgcagg ggacaaggat
     2821 aataaccgga gataattatt catcacagta tcaggatttg tttgaagaga ggatcaatga
     2881 gcttattgat gaaagtctgg cgatgagtgg tgaacgacgt tgccttcact tttctcctca
     2941 ggctgccaga atctggacgg attattacaa tgatgtggaa tcgaagctgg ggggattggg
     3001 gcctttaaga cattgccggg aatatgctgc taaaaatgca gagtatatgg caagactggc
     3061 aggacttatt taccattcga gcggtgaaga gggggagatt tccccttaca ctgcagaaat
     3121 ggcgagagaa ttagcaatat ggtacggtaa tgagtatgtg cggttgtcta atccgttaac
     3181 ttttgacaac tctgccctga ccgtacctgt gcgactcatt ccagaggagc ttgaactttt
     3241 caactggata aaaagctatt gtattgagaa ggggatcctc tgtatgaaaa aaaatgacat
     3301 tttacagcgt ggtccgaacc gtttccggaa aaaggataaa atcaactggt tactggattt
     3361 actgtatgaa caaaacagag ttgtaccggt tattgaggga aaaacgttgt gtgtcgcacc
     3421 taactttgac ctctgaacga cttaatcgtt gagtacatat agtctcctct gctacttgcg
     3481 gaggagacta atgtctttac catgtcagcg ttgtattaga aaactctggt acaggtcagt
     3541 cagtaaatat aactggtgta accactattt actattggat agagtgttgc tcaggatatt
     3601 gtcggcttta gtctgaggtg aggtggggcc ttcattgttt tcgtgttcaa taaataattt
     3661 aggttaatta gtattattag tggaagttat gtgatctgtt atgttgtctt gtacgcactt
     3721 aacactggtt acgtaccgga acactattag agtatgttat ggctctgtta cagtttatag
     3781 gtatgaaaaa atataatctt tcctgtatga aataatattc ttatggtgtg tggggtatgg
     3841 tctgtatacc atcattttta tggtgaagtt aaatatctgt agaggcctta agctgggtcg
     3901 ctgtttttac ttaatggtac agaagtatta atagcggtgt tgcctgtggc tacccgatac
     3961 tggcttttgt gaatgtcaga ttacttcctg taagtgattg aatggtactt ccttaaactc
     4021 aattctatga ggtgtgtttt taccattgta attcgtactg gtcctcagtt taagtgagtc
     4081 ttactgtact ctgaaatatt acctctttgg agtaatgagg taatacgaaa cagttttatc
     4141 aggttgtctg tgctgctggt tacctcgttt attaatgccg cggtacggcg tttctgtact
     4201 ctcagtattt atgtaattcc accttcctgt aacttttata aagtaaggac tataaaatgt
     4261 ataatgctaa tcccaattac gaaatggact tcatgattct gaaagacgtg aatgaacaca
     4321 tggaaggtct gtttcaacgt ttttctaaac tgttaccttt cagaattgat tttgcctaca
     4381 ggaaagacac gcccagtttt ggtcattcct gtaggcacag tatgtgtatt gaaatgcacc
     4441 gcttacttag tgaaacccaa acgatgctgg cgggatatta ctgggtaatg gaatatacgt
     4501 caaacaaggg acttcatatt cattttatcg gctacctgga tgggcaacgt cacaacaaat
     4561 cttatcggat atccaggcaa ctgggagata tctggaggcg gatcacggaa ggggagggtt
     4621 attttcacct gtgcagggct aaagacaaat acccggtgcg tatcgatcac gtaattcact
     4681 atagtgataa atctgcggta gacgacttac gttacgccct tagctatctg gccaaacagg
     4741 atcagaaaga acacggtatt attctgaggc gtagcaggct tcccgaaaaa agtaaccggg
     4801 gccgaccacg acataactga attacagctg tattaggtat tagaattaag tttctgtaag
     4861 ttgttgggag gtgcttgagt taataggcag aagagggcgg ggcgctttgt catcagtgta
     4921 ggggacccga atttctgccg tggtgcctca gtggatcgga tacaggtaac gaatatggtt
     4981 cataaatctg acagtgatga attagctgca ttgagggcag aaaatgcccg tctggtctca
     5041 ttacttgaag ctcatgggat tgaatggcga cgtaaaccgc agactcctgt gcagcgcgtt
     5101 tctgtattat ccaccgacga gaaggttgca ttatttcgtc ggttgtttcg tgggcgtgat
     5161 gatgtatggg cacttagatg ggaaagtaaa accagcggca aatcagggta ctctcctgcc
     5221 tgcgctaacg aatggcaggc gagaatatgc ggtaaacccc ggataaaatg tggggactgc
     5281 gctcaccgtc agttgattcc tgtatccgat ctcgtcatct accaccatct ggccggtacc
     5341 catactgtcg ggatgtatcc gttgctggaa gatgattcct gttattttct ggcagttgat
     5401 tttgatgaag ctgaatggca gaaggatgca tccgcattca tgcgatcctg tgatgagctg
     5461 ggtgtacctg ctgcgctgga aatatcccgt tcacgtcagg gggcgcatgt ctggatattt
     5521 tttgcctcac gagtttcggc ccgcgaagct cgccgtctgg ggactgctat tattagctat
     5581 acgtgtagtc ggacccgaca gctgcgattg gggtcttatg accgattatt tcctaatcag
     5641 gatactatgc caaaaggggg atttggtaat ctcatagcgt tacctctgca aaaaagaccg
     5701 cgtgaattgg ggggaagcgt ttttgttgat atgaatctcc agccttatcc tgatcagtgg
     5761 gcttttcttg tatcggtgac cccgatgaat gtgcaggata ttgaaccgac gatattacgg
     5821 gctacaggga gtatccatcc tctggatgtg aattttatca acgaagaaga cctgggtacg
     5881 ccgtgggaag agaaaaaatc atcaggaaac agactgaata tttctattgc agaaccgctg
     5941 aaaatcacgc tggcaaacca gatctatttc gaaaaagcgc aattacctca ggtgctgatt
     6001 aaccgactta ttcggctggc agcatttccg aaccctgagt tttataaggc tcaggcaatg
     6061 cgtatgtcag tctggaataa gccccgtgtt ataggttgtg cggagaatta cccgcaacac
     6121 attgcgttgc cacggggatg tctggacagc gtattatctt tccttaggga caacaatatt
     6181 gctgcagaat taatcgataa acgatttgcc gggacggaat gtaatgccgt ttttatggga
     6241 aacctcagag cggagcagga agaggccgtt tcggcattac tccgttatga cactggtgtg
     6301 ctttgtgcgc caacggcttt tggtaagaca gttaccgcag cggcagtgat tgccaagcgg
     6361 aaagtgaata cactgatact ggtacaccgg actgaattgc tgaagcagtg gcaggagcgt
     6421 ctcgcggtgt ttctgcaggc cggtgacagt attggtatta tcgggggagg taaacataaa
     6481 ccctgtggca atattgatat tgcggtggtg cagtccatat ccagacaagg agaagttgaa
     6541 cctctggtca ggaattatgg gcaaatcatt gtggatgagt gccatcatat tggcgcggtt
     6601 tcattttctg cgattctgaa ggaaacgaat gccagatatc tgcttggcct gacggcaaca
     6661 ccaatccgac gggatggtct gcatcccatt atttttatgt actgtggtgc cattcgccat
     6721 acagcggtcc gcccgaagga aagcccacat aatctggagg tactgatccg ttcccgtttt
     6781 acatctggtc atttaccatc ggatgcgaga atccaggata ttttcagaga aattgctctg
     6841 gatcatgaca gaacggtggc gatagctgaa gaagccatga aagctttcgg gcaggggcga
     6901 aaagttctgg tactgactga acgtacagat catctggatg agatagcatc agtgatgaat
     6961 tcactgaaat tgtctccctt tattctccat ggtcgactat cgaaaaaaaa gcgtgcgatg
     7021 ctgatatccg ggctgaatgc tcttcctccc gattctcctc gaattttgtt gtcaacaggc
     7081 agacttattg gtgagggatt tgaccaccct ccgctggata cgctgattct tgccatgcct
     7141 gtgtcatgga aagggacatt acagcagtat gcagggcgtc ttcacagaga gcataccggt
     7201 aaaagcgatg tcaggat
//
LOCUS       DAZGTR010000185         7184 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGTR010000185 DAZGTR010000000
VERSION     DAZGTR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.187"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(191..2974)
                     /locus_tag="ACHLCY_004531"
     CDS             complement(191..2974)
                     /locus_tag="ACHLCY_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0371631.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVHDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3031..5403)
                     /locus_tag="ACHLCY_004532"
     CDS             complement(3031..5403)
                     /locus_tag="ACHLCY_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP0371632.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSVVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5441..7126)
                     /locus_tag="ACHLCY_004533"
     CDS             complement(5441..7126)
                     /locus_tag="ACHLCY_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP0371633.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQSGVWNLADDICDISAVL"
BASE COUNT         1870 a         1635 c         1598 g         2081 t
ORIGIN      
        1 tggtggtaag gtgttttatg ctgttatttt tatgcgcatt ctgtgtctcc tgaattatca
       61 cgtaaaaatc agaccttaaa atatcactat tagtacttga ttattatttt gaacgcattt
      121 ataaaattat tacataaaaa tagcgaatat tgctaaaatc cccgccaacg atgtgttgac
      181 ggggctgtta ttattttggc aataatactc cggtataagt atttaccgga tgagaaagat
      241 attgtttaac ggcagtgtta acattctcta ccgtcatttg tttcaacaat tgctcctgct
      301 cagtccatgc tgcaggatcg tcatattgaa taagactatt tacaatagtg ttcgctaatt
      361 gttgaacgct acgctgttgg atatcgaggc tgcgctgaac gttttgctgg tattcattca
      421 gttcttgctc actgatccct ttagccagac gcttaaccat cacttcattc gctaacgtta
      481 acagttcatc atgtcgttct ggttgacaag taaaagccag caaatgactg atatctttgg
      541 cctgaggatc aaccgagagg cgagaagaaa cgctgtatgc tccagatgcc tgttcacgaa
      601 tattaacacg tagatctttt gccagtgcga cgttaaaagc atcgagcgcc atacgcgtcg
      661 ccagattaac aggtgtccgg gaatcataac gcttccactg tgaaacctgt gccacaggtt
      721 cattttgttc ttttacagta accgatgcgt tgtccgtcgc gcgagttaat ggtttacctg
      781 cggctaatgg cgaatcagag tgtttgattg atcctaagta acgcgtaatt aacgccacga
      841 gtttgtcttc tgagacatta ccgacaatga caaacgtgat atccgctgga gatgaaaaca
      901 actggcgatc ggcagccagc gcatctgcgg cagtaaactg tacaatctga ttttcttgca
      961 gtaatttcgt gcggtcatca gcatagcgcg tctcatacat ctgctgggcg aatttctccg
     1021 ctggacgctg gtcgagcgtt ttcaacgcct gaatttgagc attttgtagc gatgcccaaa
     1081 tattgtcgtt aatcgtgctg tgggtgattc gctggttaat caactgaaaa ccaggttcag
     1141 ggttattagt ccgcgcgcta acggagagca acgtattcat gccactgact ttactgctca
     1201 tggttaccga attttccgca ctccagcgtt tcaggctgga agaggagagc tcaccaacgc
     1261 cgcttccgct tactgcttta tttgccagcg cgataagtga tttttgctgc gcagggaaac
     1321 ttaaatcgcc tttattcgat acggcagtaa tttgtagctt ttgctcttca cccgcggatt
     1381 ttgccagaat aaccctggca ccattggaaa gtgttaatga tgtcagattc tcagccagcg
     1441 tttctttgct gctaatttcc gcctgtgggt cagcgtctac tgttaacgat aaatttctgc
     1501 ctgggaagat gtaagccgcc agctttttgt tggcgtactc tttttccagc gccaggatag
     1561 ccgcaggaga caatgctttt ttggcggcaa gctcattgtt taccatttgc tcccaaaatg
     1621 cgtcctggtt ctttcttaac tgctgccatt tttccgccag actttgcacg gtaatttgct
     1681 gccacagacg tttcgaaagt tgatatgtct cttccggcga caagaacggc gtattattta
     1741 atgagctgga tgccaggcga ctggtcagca tacgtaaatc acgttcagct tgctgatcaa
     1801 ccgcattttt cagccaggtg aggcgggtag atttaacatc atcgagttct tcagcagaaa
     1861 aaccatgctg atcaatagtt gccaactctg ccattaatgc attcgcagca tcctgcatat
     1921 tatcgtctcg tgcatttaca cggaaaaaca gcgactgata atcgggtgca attttaacgc
     1981 tgcgcgcagt gccgccagaa atagtcttca actcgcccga ctgtatgcgt tcctgcagac
     2041 gttgattgaa cagctgaact aacatgctcc attcagcttg ttcgataaag ctttgctcat
     2101 cgtgcacttg taccattggc aggcgataat agagtgcgat gccgttcacc cggttttctt
     2161 tatcattaat gatattaaag cgcaggtggt tttcggcttt tgtcggccag acgcgatttt
     2221 cagctgcttt gttagccgga agcttactta aattatcctt tatcagcgcc agcgcttctt
     2281 tactgtcgat atcgccaacc acgataaagg tcatattatt tggttgatac cagcgttgat
     2341 aaaattggcg caattgtgcc ggtgtgaccg tggcgacagt atccatcagg ccgataggtt
     2401 cacggtctaa attacgggta ttagccagca ggaaagggcg gcgcgcctga gaggtgcgcc
     2461 atttcgcatc ctgatgggca cgccattcct cagtaattac gccacgttca gcgtctactt
     2521 cgagtttttc aaaggttgcg gcattactcc attcactgaa gattgccatc acttgttgca
     2581 gattttgttt ctgcgtagtc ggcaaactca cctgatacac cgtttcgtca tagctggtat
     2641 aggcattaac atcgcgacca aaacgcaggc ccattgactc aaatgtttcg atgactttat
     2701 tacccggcca tgtttttgtg ccgttaaaca tcatatgttc tacaaaatga gccacgccgc
     2761 gctcattgtc ttcttcctgc aatgaaccgg tatgaatttg cagccataaa tttacctgat
     2821 cttttggatg agcatgcgga taaatcatat atcgcaagcc attgcccagt tgcccggtaa
     2881 ttaacttttc atcctgcggt aaggcggcgg caatcagccg cccggggagc aacagagttg
     2941 ccactaacgt cagtaagaaa cagaggtttc tcataattat ctccatgcga aaaccgggcg
     3001 aatttacccg gttaagtaaa atccgaacta ttaaaatttc atgctgacat caagccagaa
     3061 agtacggcca ctggcgtagg tcgccacacc ggtattcgtt gtatcaacag cggttttgct
     3121 atccaataca ttgagaatat cggcactgat cgtcaggttt tgttgtttca gaaattgtgg
     3181 cgtccaggac aagcgggtgt cccaggtcag gctgctatcc agtttttcgt caacatactg
     3241 cttgtaatct gaatattcgc tgatgtattg cgcatttgtc ttacccagga taatgcgagc
     3301 tttacgcgct tcttgccagg ccagcgtatt cgcccacact aacccgctcg gttgatgcgt
     3361 gaaatccatg tttaaggaga tctttaatgg gttattaaaa tctgccactg gaacgctatc
     3421 gtaagaaacc agattaccgt tataaaccac ctggttatca ccggtattgc tctcctcata
     3481 accattgttt aacgacaagt tgcccttgct cttgatatag ctaaagacaa tttgcgggtt
     3541 aatatctacc tggctgatat gcaggggttc ggccagttca aaactgaggt tgaacgaatg
     3601 cgtttttgtt ttgccgtcgt tgttatattc agtaatggtg gttttagtcg cgctgtcggt
     3661 acgactgctt ttgctgattt gatcatgcgc ttcacggtaa acatagtttg cgcgcgcaat
     3721 aacgttctta ccgattttct gctgcaatcc cattgccagt tcatcgttat aaggcgtttt
     3781 caaatcctga taacgcgtca gggttttatt gcctgatacc gattccgtcc agctattgcg
     3841 gatatcacgt aatcccatat caagaatatt cccgccatag taacggttat aacctgctgt
     3901 aatcattgag gtttgatcag caaaaatatc ccattccgtc ataaagcgcg gggagatatt
     3961 gtggtttgac agatagttgt catagtcata acgcacaccg ggcattaacg acacattccg
     4021 ccagctaatg tgatccgcca tatacagcgt gtagttgtca attcccaggc tgcctttacc
     4081 tttatggtaa atggtatggt tagttttctt tcctgcagca ttaatcacat aggattcaga
     4141 ctggttatgg cgttcagtcc atgcatcgga atagatgtat tccgcgccga agtagggttg
     4201 atgcgaaaca tcacccacgg cgaatttttg ccagtccagg cgtgttttga aggtgtaatt
     4261 atctacagcc tgggaaatgt gtcctaatcc gccacgggta caacgccctg taatatcacc
     4321 atatgtacat gaaagttcgg tgtaccagat gtcatgatcg tgacgggtat aatcactaat
     4381 atgatcccaa ccaacggtgg tacgcagttt ggcccatgcg agctgcgtat ccatatccca
     4441 tgccagaccg taggatttat tacccatttc gcgatcagac tgcgggaagg tgctggtatt
     4501 ataatcacgg ctggagccgg tatattttaa ggttaaatcg tgggtaaagc ggtcgctggc
     4561 aaaccaggta aatttgctca atgcagtatc gataacgttt ttatactgtg cccgaccggc
     4621 gacaatgccg tcattcgaaa cataatccgc gcgggtgata tcagactggc ggcgcgataa
     4681 accggcggta acgccgaagt tatcagcgag ttcctgatta aacgacaagg tataaaagtt
     4741 ctttttaaaa tctggggagt aataagtact tcctgaagag ccttgattaa atgcgctctt
     4801 gttattctca tcgatatgcg atgttaacca gtccgaacgc gtagtgcgat aacccaattt
     4861 caccttgcta tcatcagcgt tgaagcgttt gatctttgca tcaattaccc cgccattgaa
     4921 gcgaccaaat tcaaccggca caacactgtc ataaagcgtc acattgtcca gtaagctgac
     4981 atcaagataa tacccctgtg acatcccgct aatattggtt gcactactgg catcggactc
     5041 attcgctggg ttcaagttat tagtggcact aataccgtca atcaaatagg cattctgata
     5101 gggcgacgca ccgtgaatag agattttctc cgggcgaata tctccctggt tcaacgaggt
     5161 actttgcgtt gaatccatgc gtaccgcagg gttggttctc agcagatcgc tgatattgcc
     5221 attcccggtc ggtaaacgct cgatgctttc actggtgtaa tgcgtgttgc cgtttacggg
     5281 tacagggaca ggagcataga ccgtcatctc ttcaaaaaca tgcatgtaca tgtttttgtc
     5341 atcgacggcc tgcgccacat cagcgccaca taaaatgacg ccaggaataa gaactcgctt
     5401 catttattta tatctccgta cagcagggct tttattgttt ttataaaact gcgctaatgt
     5461 cacaaatatc atctgccagg ttccagacac cggattgatg tgtaaccata atgacgccgc
     5521 ttgtgggtag tttttcacgc actaaacgca gtaagcggat agcctcttgt tcctcaagat
     5581 gagaggtagt ttcgtcaaga aatatccatt tcggacgtcg taaaattaat cgtgccaggg
     5641 cgatacgttg tttttcgccg ctggaaagaa tatctcccca gcgatcgtgg tcatgaatac
     5701 gcgcagccaa tttccccaga ccaacctgat gcagtacttc gctcaacgat ttatcgtcta
     5761 cgggcagggg aagtgcttta caaataatct ctttcagtaa gccggttttg attaacggtg
     5821 tttgtgacac ataccaactg tcagcaggag aagaaatatc acctttaaac cacggccagc
     5881 agtgagataa tgttttaagc agtgtggttt ttcccgcacc agagtaacct ttcagtaata
     5941 gccatttgcc tggcgaaaca tgaaagttca ggttctctaa tatgatctta ttatcaggag
     6001 tacgaatact cgcgtcagcc acttgtaccg catgttggca atttttaggt ttattcgtag
     6061 ggcgctgttc ggtgagttga tggaactcat acaaacgatc gataaccgca gccagttcag
     6121 caagttcgtc atatttataa ataaaccagc ttaaattgtt cgataccagc ataaatgcct
     6181 ggcgcgattt catcagtccg cccagattga tctgcccgct aataaactgc ggcaataaca
     6241 gaaagtaggg aagaacgcta agcgagcgcg aataaatatt ctgccagtaa tcaagccacc
     6301 gctgacgatt cattaaacga tgccagttct ctttaatggt atgaaaattg tcgctcaact
     6361 cctggcgttg tagagactca gcattgctta gcgcaatcag ttctgcctgc ttattatgct
     6421 gcacaagatt tgttcgaaat gtcgcttcgc tacgttgttt ttccacatta agcggacgaa
     6481 tacgtttacc aaccttatgg gtaaataaag ttccaccgat cacaatgagc acaacggtat
     6541 agaccatata cccctggata ttccattctg ttccaccaac agtgaatgag agcgtacccg
     6601 cgctttgcca aagaataacg gtaaaggtga tcagcatgct aagtgactgg atgaagccaa
     6661 atgaaaggct gagcgttttg ctgatcagta agagaatgtc ttcagcgata cgttggtcag
     6721 ggttatctgt atttttatgc tcgccgtaga tctgcgtgaa gtagtagttt ttatctgcaa
     6781 accaccgatt aaggtagtaa tctgttaacc attcgcgcca gcgaatagtc agtagtttaa
     6841 ttaaccatgt tttgtttacg gatattaata caaagatccc cagcagggca gggaaccaaa
     6901 ggacaagctg ccaaagctta tcggtttctt tctggcttag cgcattgaaa aaatcattat
     6961 tccagtcgtt taaccagacc tgaattttaa ccacgccaag gatcatggcg aggataataa
     7021 tgattaacaa cactgaagtt ttattattct tacgcagcca aaagggcttc agcagacaga
     7081 gatactttgc tatcaacata caaaacgtaa tgggaatggt tatcattagc gaaaattgat
     7141 tgtgccatcc aatgattatc tatggcaatg atttactgat gaaa
//
LOCUS       DAZGTR010000186         7171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGTR010000186 DAZGTR010000000
VERSION     DAZGTR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.188"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..853
                     /locus_tag="ACHLCY_004534"
     CDS             <1..853
                     /locus_tag="ACHLCY_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFP0371634.1"
                     /translation="EITLTVNPDYKYSGTTDDKSKRMQSDSLSSLLSYAIGCMMGRYS
                     LDREGLVYAHEGNKGFADLVAEGAYKTFPADSDGILPLMDDEWFDDDVTSRVKEFVRT
                     VWGEEHLQENLEFIAESLCLYAIKPKKGESALETIRRYLSTQFWKDHLKMYKKRPIYW
                     LFSSGKEKAFECLVYLHRYNDATLSRMRTEYVVPLLARYQANIDRLNDQLDEASGGEA
                     TRLKRERDSLIKKFSELRSYDDRLRHYADMRISIDLDDGVKVNYGKFGDLLADVKAIT
                     GNAPEVI"
     gene            1030..3627
                     /gene="pglZ"
                     /locus_tag="ACHLCY_004535"
     CDS             1030..3627
                     /gene="pglZ"
                     /locus_tag="ACHLCY_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-1 system phosphatase PglZ type A"
                     /protein_id="HFP0371635.1"
                     /translation="MQNQDFIAGLKAKFAEHRIVFWHDPDKRFIEELEQLKLENVTLI
                     NMTHESQLAVKKRIEIDEPEQQFLLWFPHDAPPHEQDWLLDIRLYSSEFHADFAAITL
                     NTLGIPQLGLREHIQRRKAFFSTKRTQALKNLVTEQEDEASLDKKMIAVIAGAKTAKT
                     EDILFNLITQYVNQQTEDDSELENTQAMLKHHGLDSVLWEMLNHEMGYQAEEPSLENL
                     LLKLFCTDLSAQADPQQRAWLEKNVLLTPSGRASALAFMVTWRADRRYKEAYDYCAQQ
                     MQTALHPEDHYRLSSPYDLHECETTLSIEQTIIHALVTQLLEESTTLDRETFKKLLSE
                     RQSKYWCQTQPEYYAIYDALRQAERLLNLRNRHIDGFHYQDSATFWKAYCEELFRFDQ
                     AYRLFNEYALLVHSKGAMILKSLDDYIEALYSNWYLAELSRNWNEVLEAENRMQAWQI
                     PGVPRQQNFFNEVVKPQFQNPQIKRVFVIVSDALRYEVAEELGNQINTEKRFTAELRS
                     QLGVLPSYTQLGMAALLPHEQLCYQPGNGDIVYADGLSTSGIPNRDTILKNYKGMAIK
                     SKDLLELKNQEGRDLIRDYEVVYIWHNTIDATGDTASTEDKTFEACRTAVTELKDLVT
                     KVINRLHGTRIFVTADHGFLFQQQALSVQDKTTLQIKPENTIKNHKRFIIGHQLPADD
                     FCWKGKVANTAGVSDNSEFLIPKGIQRFHFSGGARFVHGGTMLQEVCVPVLQIKALQK
                     TAAEKQPQRRPVDIVAYHPMIKLVNNIDKVSLLQTHPVGELYEPRILNIYIVDNANNV
                     VSGKERISFDSDNNTMEKRVREVTLKLIGANFNRRNEYWLILEDAQTETGYQKYPVII
                     DLAFQDDFF"
     gene            3638..5722
                     /gene="brxL"
                     /locus_tag="ACHLCY_004536"
     CDS             3638..5722
                     /gene="brxL"
                     /locus_tag="ACHLCY_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HFP0371636.1"
                     /translation="MQTHHDLPVSGVSAGEIASEGYDLDALLNQHFAGRVVRKDLTKQ
                     LKEGANVPVYVLEYLLGMYCASDDDDVVEQGLQNVKRILADNYVRPDEAEKVKSLIRE
                     RGSYKIIDKVSVKLNQKKDVYEAQLSNLGIKDALVPSQMVKDNEKLLTGGIWCMITVN
                     YFFEEGQKTSPFSLMTLKPIQMPNMDMEEVFDARRHFNRDQWIDVLLRSVGMEPANIE
                     QRTKWHLITRMIPFVENNYNVCELGPRGTGKSHVYKECSPNSLLVSGGQTTVANLFYN
                     MASRQIGLVGMWDVVAFDEVAGITFKDKDGVQIMKDYMASGSFSRGRDSIEGKASMVF
                     VGNINQSVETLVKTSHLLAPFPAAMIDTAFFDRFHAYIPGWEIPKMRPEFFTNRYGLI
                     TDYLAEYMREMRKRSFSDAIDKFFKLGNNLNQRDVIAVRRTVSGLLKLMHPDGAYSKE
                     DVRVCLTYAMEVRRRVKEQLKKLGGLEFFDVNFSYIDNETLEEFFVSVPEQGGSELIP
                     AGMPKPGVVHLVTQAESGMTGLYRFETQMTAGNGKHSVSGLGSNTSAKEAIRVGFDYF
                     KGNLNRVSAAAKFSDHEYHLHVVELHNTGPSTATSLAALIALCSILLAKPVQEQMVVL
                     GSMTLGGVINPVQDLAASLQLAFDSGAKRVLLPMSSAMDIPTVPAELFTKFQVSFYSD
                     PVDAVYKALGVN"
     gene            5841..>7171
                     /locus_tag="ACHLCY_004537"
     CDS             5841..>7171
                     /locus_tag="ACHLCY_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HFP0371637.1"
                     /translation="MKLIANGLNKQFFSSFLPPPDTEIDGVVAAIAYGDDKTSLLDHC
                     IKNHHRLDIWMRYDHTVPVSPAFLSKLLANVKNNIFCKLVPDCLHCKIIWWKGYGAYI
                     GSANLTGRAWYSNIEAGVFYDENDLYNTGLIEQLEEFFDNLSDLDSCIELTKEIIQEQ
                     QQLQKLKLEQEKKEQAIIKKRLIPEWAGVSNYDKIKSSDKRKDAFRKEWESTLSTIRN
                     ISSQINDFRPAWISEDTPAFWQTDQFLHAYYYNQVRRNDKTFPYEEDYQNNRKNPQAA
                     LMNMLSWWKSLSEPPSHEDINLGINANYIREHLAKDKINTLSEEELHKIFSYTHATMD
                     HVIKMSVDTFGLTSRISLDKEKRAILFTQWIMKQTNKNGMTIAELLNYVLYDGKQELM
                     WERIYLAGKDDNYKFQHYGINSISEVVGWARPEVTPPRNGRTNKALRALGYP"
BASE COUNT         2006 a         1693 c         1744 g         1728 t
ORIGIN      
        1 agaaataaca ttaacggtaa atcctgatta taagtattct ggaaccacgg atgataaatc
       61 aaaaaggatg cagtcagaca gtctttcctc cctactatcc tatgccatcg gctgcatgat
      121 gggccgctac tccctcgatc gcgaaggcct tgtctacgcc catgaaggta acaaaggctt
      181 cgccgatctc gtcgccgaag gtgcctacaa aaccttcccg gccgacagtg acggtatcct
      241 gccgctgatg gatgacgagt ggtttgacga cgacgtcacc tctcgcgtca aagagtttgt
      301 ccgcaccgtc tggggcgaag agcacctgca ggaaaacctc gaatttatcg ccgaaagtct
      361 ctgtttatac gcgatcaagc caaaaaaagg cgaatctgcg ctggagacca ttcgccgcta
      421 tctttccacc cagttctgga aagatcatct gaaaatgtac aaaaagcgcc ctatctactg
      481 gctgtttagc tccggtaaag agaaagcctt cgagtgcctg gtgtatctgc atcgctataa
      541 cgacgccacg ctgtcgagaa tgcgtaccga atatgtggtg ccgctgctgg cgcgttatca
      601 ggccaatatc gatcgcctga acgatcaact tgatgaggct tcaggcggtg aagctactcg
      661 cctgaaacgc gaacgcgata gcctgatcaa aaaattcagc gaactgcgca gctatgacga
      721 ccgcctgcgt cactatgctg atatgagaat cagtattgat ctcgacgatg gcgttaaggt
      781 taactacggc aagtttggcg atctgctggc agatgttaaa gccatcaccg gcaatgcccc
      841 agaggtgatc taaaccagac ggcgcgttct cctgctgccg ggttctgccc ggtggcaaat
      901 accaccggga aacgcgccgc tgctggcatt tctccacctc acttcatgat aaaatgcgcc
      961 atcgtgtcaa aatctccttt tcacgttttg gcgctttctt attcatcgta acaacatggg
     1021 attgtgaact tgcaaaatca ggactttatt gctggcctta aagctaaatt tgccgaacat
     1081 cgcatcgttt tctggcacga tcccgataaa cgttttattg aggaactgga acagctcaag
     1141 cttgaaaacg tcacgctaat caacatgacc cacgagtcac agctggcggt gaaaaaacgc
     1201 atcgagattg atgagccaga acagcagttc ctgctgtggt tcccccatga tgcgccgcct
     1261 catgaacaag actggctgct ggatatccgc ctttacagca gcgaattcca tgccgatttt
     1321 gccgccatca ccctgaacac gctgggcatt ccccagcttg gcctgcgcga gcatattcag
     1381 cgacgcaagg ccttcttcag cactaaacgc acgcaggcgc tgaaaaactt ggtgacagaa
     1441 caggaagatg aagcctcgct ggataagaaa atgattgcgg tgatcgctgg cgcaaagacc
     1501 gcgaaaaccg aagacatttt gttcaatctg attacccagt acgttaacca acaaacagaa
     1561 gacgacagcg aactggaaaa cacgcaggcg atgctgaaac accacggtct ggactcggta
     1621 ttgtgggaaa tgctcaacca cgaaatgggc taccaggcag aggagccatc gctggaaaac
     1681 ctgctcctga aactgttttg taccgatctc tctgcccagg ccgacccaca gcagcgcgcc
     1741 tggctggaaa aaaatgtcct gctgacgcca tccggcagag catccgccct ggcatttatg
     1801 gtgacctggc gtgccgatcg tcgctataaa gaggcttatg actactgcgc tcagcaaatg
     1861 cagaccgccc tgcacccgga agatcattac cggcttagct cgccatatga tttgcacgaa
     1921 tgcgaaacca ccctcagcat cgaacaaacc attattcatg cgctggtaac gcagctgctg
     1981 gaagagagca ccacgctcga tcgggaaacc tttaaaaaat tgctctctga acgccagagc
     2041 aaatactggt gccagacaca acctgagtat tacgccatct atgacgcgct gcgccaggca
     2101 gagcgtttgc tgaacctgcg caatcgccac atcgatggtt tccactacca ggacagcgcc
     2161 accttctgga aagcctactg cgaagaactg ttccgcttcg accaggctta tcgcctgttt
     2221 aatgaatatg ccctgctggt tcacagcaaa ggagcgatga tcctcaagag cctggatgat
     2281 tatatcgagg cgctctacag caactggtat ctggcagagt taagccgtaa ctggaacgaa
     2341 gtgctggagg cggaaaatcg tatgcaggcg tggcaaatcc ctggcgtgcc gcgtcagcag
     2401 aactttttca atgaggtggt gaagccgcag ttccaaaatc cgcaaatcaa acgcgtgttc
     2461 gtgatagttt ccgatgccct gcgttatgaa gtggcggagg agctggggaa tcaaatcaat
     2521 accgagaaac gctttaccgc agaactgcga tcgcagctag gcgtgctccc aagctacacc
     2581 caactgggaa tggcggcgtt gctgcctcat gaacaacttt gttatcaacc gggtaacggt
     2641 gacattgttt atgctgatgg gctgtcgacc tcgggtattc ctaaccgcga taccattctg
     2701 aaaaactata agggaatggc gataaaatcg aaggaccttc tggagttaaa aaatcaggaa
     2761 ggacgagacc ttattcgcga ttacgaagtg gtgtatatct ggcataacac gattgatgcc
     2821 accggcgaca cggcatccac ggaagataaa accttcgaag cgtgccgcac ggcggtgact
     2881 gaactgaaag atttagtcac caaagtaatc aaccgcctcc acggcacacg catttttgtt
     2941 acggcggatc acggtttcct gttccagcaa caggcgcttt cggttcagga taaaacaact
     3001 ctgcaaatta agccggaaaa caccatcaag aaccacaaac gctttattat cggccatcag
     3061 cttcccgccg atgatttttg ctggaaaggt aaagtggcaa ataccgcagg tgtgagcgac
     3121 aacagcgagt tcctgattcc gaaagggatc cagcgcttcc atttctctgg cggcgcgcgc
     3181 tttgttcatg gcggcaccat gttgcaggag gtttgcgttc cggtattgca gataaaagcc
     3241 ctgcaaaaaa ccgccgcaga aaaacagccg cagcgccgcc cggtggatat tgtcgcttac
     3301 catccgatga ttaagctggt gaataatatc gataaagtga gcctgttgca gacgcatccg
     3361 gtgggcgaac tttatgaacc gcgtatcctg aacatttaca ttgtcgacaa cgccaacaat
     3421 gtggtctcgg gcaaagagcg catcagcttt gacagcgata acaacaccat ggaaaaacgc
     3481 gtgcgcgaag ttaccctgaa gctgattggc gctaacttca accgtcgcaa tgagtactgg
     3541 ttgatactgg aagacgcaca aacggaaacg gggtatcaga agtacccggt cattatcgat
     3601 ctggcattcc aggatgattt cttctaagtg aggcgatatg caaacccatc atgatttacc
     3661 tgtttcaggc gtatccgcag gggaaattgc ctccgagggt tacgatctgg acgccctgct
     3721 gaaccagcat tttgctggtc gcgtggtgcg taaagatctc accaagcaac tcaaggaagg
     3781 ggcaaacgtc ccggtgtatg tgcttgagta tctgctcggc atgtactgcg cctctgacga
     3841 tgacgacgta gtcgagcaag ggttgcaaaa cgttaagcgt attctggctg ataactatgt
     3901 gcgcccggat gaagcagaga aagtgaagtc gctgatccgc gagcgtggtt cgtacaaaat
     3961 catcgataaa gtgtcggtga aactgaacca gaaaaaagac gtttacgaag cccagctttc
     4021 taacctcggc atcaaagacg cgctggtgcc atcacagatg gttaaagaca acgagaagct
     4081 actgacgggc ggtatctggt gcatgattac cgtcaactat ttctttgaag aagggcagaa
     4141 gacttcgccc ttctcattga tgacgcttaa gcctatccag atgccgaata tggatatgga
     4201 agaagtgttc gatgcgcgta gacactttaa ccgtgatcag tggatcgatg tgctgctgcg
     4261 ctcagtgggt atggagcccg ccaatattga gcaacgcact aaatggcacc ttatcacccg
     4321 aatgatcccg tttgtggaga acaactataa cgtttgcgaa ctggggccgc gcggcaccgg
     4381 taaaagccat gtgtataaag agtgttctcc taactccctg ttagtttctg gcgggcaaac
     4441 gaccgttgcc aacctgttct acaacatggc cagtcgccag atcggccttg ttggcatgtg
     4501 ggatgtggta gcgttcgacg aagtcgcggg gatcactttc aaagataaag acggcgtgca
     4561 aatcatgaaa gattacatgg cgtcaggatc tttctctcgc ggcagagatt cgattgaagg
     4621 taaagcgtcg atggttttcg tcggcaacat caatcaaagc gtagagacac tcgttaaaac
     4681 cagccatttg ctggcgccat ttccggctgc gatgattgat accgcctttt tcgaccgctt
     4741 tcatgcctat attcccggtt gggaaatccc caaaatgcgc ccggaattct ttaccaaccg
     4801 ttacgggctg attacggatt atctcgctga atatatgcgc gaaatgcgca aacgcagttt
     4861 ctctgatgcg attgataaat tctttaagct gggtaataac cttaaccagc gtgacgtcat
     4921 tgctgttcga cgtaccgtgt cgggattgtt aaaactcatg catcccgatg gcgcgtacag
     4981 caaagaagat gtgcgagtct gcctgaccta tgcgatggaa gttcgtcgcc gcgtgaaaga
     5041 gcaacttaaa aaactgggcg gcctggagtt cttcgatgtg aactttagct acatcgataa
     5101 cgaaacgctg gaagagtttt ttgtgagcgt accggaacag ggcggcagcg aacttattcc
     5161 tgccggaatg ccaaagccgg gcgttgtgca tctggtcact caggcagaaa gcggcatgac
     5221 cgggttgtat cgctttgaaa cacagatgac tgccggtaat ggtaagcata gtgtatcggg
     5281 tctgggttca aatacctccg cgaaagaagc tatccgcgtc ggtttcgatt acttcaaagg
     5341 caatttgaac cgggtaagcg cggccgcgaa attctccgat catgaatatc atcttcatgt
     5401 cgttgaactg cataatactg gccctagcac cgcaaccagt cttgctgctc ttattgcttt
     5461 atgttcgata ttgctggcaa aaccggtgca ggagcagatg gtggtgttgg gcagtatgac
     5521 gcttggtgga gtaattaacc cggtacaaga tcttgctgcc agtttacagc tcgccttcga
     5581 cagcggcgca aaacgagttc tgttgccgat gtcctcggct atggatattc caacggttcc
     5641 ggcagagtta tttaccaagt ttcaggtgag tttttactca gacccggttg atgcggttta
     5701 taaggcgctg ggagtgaatt aacatatatc gagggatgga tcacattcct cggtatattt
     5761 gtcccaggat tattcttatt tgatataata tcagttctaa ttaacttatt atcaatggaa
     5821 tatttctcgg ctaaataaca atgaaactta tcgctaacgg attaaataaa caatttttta
     5881 gttcattttt accacctcct gatacagaaa ttgatggtgt tgtcgctgcc attgcttatg
     5941 gcgatgataa aacctcgctt ttggatcact gcataaaaaa ccatcacagg ctcgatattt
     6001 ggatgcgata tgatcatact gttccggtat cgcccgcatt cttatcaaaa cttctcgcca
     6061 atgttaaaaa caatatattt tgtaaactcg taccagactg cctgcactgc aaaataattt
     6121 ggtggaaagg atatggtgct tatattggct ctgctaattt aactgggcgc gcgtggtatt
     6181 cgaatattga agctggtgta ttttatgatg aaaatgatct gtataacaca ggattaatag
     6241 aacaacttga agagtttttc gacaacttat ctgaccttga ctcttgtatc gaacttacta
     6301 aagagataat tcaagaacaa cagcagttac aaaaattaaa acttgaacaa gagaaaaaag
     6361 aacaagcaat cataaaaaaa cgacttatac ccgaatgggc tggtgtaagt aactatgata
     6421 agattaaatc aagtgataaa cgcaaagatg ctttccgtaa agaatgggag agtacacttt
     6481 cgacaatacg aaatatatcc agccagataa acgatttcag gcctgcatgg atatcagaag
     6541 ataccccagc attctggcag acagatcagt ttttacatgc gtattattat aatcaggttc
     6601 gtaggaatga taagacattc ccttatgaag aggattatca gaacaacagg aaaaacccac
     6661 aagcggcact gatgaatatg ctgtcatggt ggaaaagtct ctctgagcca ccttcccatg
     6721 aagatatcaa tcttggtata aatgctaact atattcgtga gcacttagca aaggataaga
     6781 ttaatacctt atccgaggaa gagttacata aaatattttc gtatacacat gcaacaatgg
     6841 accatgtgat aaaaatgagc gtagatacct ttggattaac gagtcgtata tccctagata
     6901 aagaaaaacg ggcgattttg tttactcagt ggataatgaa acaaactaat aaaaacggta
     6961 tgacgattgc agaattactg aactatgtac tttatgatgg aaagcaagag ctcatgtggg
     7021 aaagaatata tctggcagga aaagatgata actataagtt tcagcattac ggaatcaact
     7081 caatatccga agttgtcggc tgggcgcgtc ctgaagtgac accgcccaga aatgggagaa
     7141 ctaataaggc gttaagagct ttaggttacc c
//
LOCUS       DAZGTR010000187         7120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGTR010000187 DAZGTR010000000
VERSION     DAZGTR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.189"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(86..619)
                     /locus_tag="ACHLCY_004538"
                     /pseudo
     CDS             complement(86..619)
                     /locus_tag="ACHLCY_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            complement(624..788)
                     /locus_tag="ACHLCY_004539"
     CDS             complement(624..788)
                     /locus_tag="ACHLCY_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371638.1"
                     /translation="MSGWCRPEIMILTMKVKCIGSLIGRSEAAVRMKAQVKGISLILR
                     GDFHQSTKYP"
     gene            complement(785..1519)
                     /locus_tag="ACHLCY_004540"
     CDS             complement(785..1519)
                     /locus_tag="ACHLCY_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0371639.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KRVYTWRVADNNVQQEQPAPEETTTATEAKISECDLTATIEQRGPQTADELATLFGTT
                     SRKVASTLAMAISKGRLIRVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGV
                     ALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKW
                     ERVCAALRELNKHRDMVAGICRKSGQ"
     gene            complement(1553..2014)
                     /locus_tag="ACHLCY_004541"
     CDS             complement(1553..2014)
                     /locus_tag="ACHLCY_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0371640.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(2007..3047)
                     /locus_tag="ACHLCY_004542"
     CDS             complement(2007..3047)
                     /locus_tag="ACHLCY_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0371641.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(3019..3570)
                     /locus_tag="ACHLCY_004543"
     CDS             complement(3019..3570)
                     /locus_tag="ACHLCY_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0371642.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(3554..3780)
                     /locus_tag="ACHLCY_004544"
                     /pseudo
     CDS             complement(3554..3780)
                     /locus_tag="ACHLCY_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
     gene            3857..4264
                     /locus_tag="ACHLCY_004545"
     CDS             3857..4264
                     /locus_tag="ACHLCY_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371643.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            4529..4828
                     /locus_tag="ACHLCY_004546"
     CDS             4529..4828
                     /locus_tag="ACHLCY_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371644.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4901..5119
                     /gene="ydfC"
                     /locus_tag="ACHLCY_004547"
     CDS             4901..5119
                     /gene="ydfC"
                     /locus_tag="ACHLCY_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP0371645.1"
                     /translation="MQKRAPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            5142..5549
                     /locus_tag="ACHLCY_004548"
     CDS             5142..5549
                     /locus_tag="ACHLCY_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371646.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(5527..5760)
                     /locus_tag="ACHLCY_004549"
     CDS             complement(5527..5760)
                     /locus_tag="ACHLCY_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371647.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
     gene            6321..6509
                     /gene="dicB"
                     /locus_tag="ACHLCY_004550"
     CDS             6321..6509
                     /gene="dicB"
                     /locus_tag="ACHLCY_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0371648.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            6506..6694
                     /locus_tag="ACHLCY_004551"
     CDS             6506..6694
                     /locus_tag="ACHLCY_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0371649.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            6790..>7120
                     /locus_tag="ACHLCY_004552"
     CDS             6790..>7120
                     /locus_tag="ACHLCY_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFP0371650.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLS"
BASE COUNT         1805 a         1817 c         1573 g         1925 t
ORIGIN      
        1 cttatccgac tttacctcgt gcatagcttg ttatttaaag tcaacaaaat aaggaaaatt
       61 atacgcatat tgaagagtat aaaccttaca tgttgattac atttttgtaa tcaacatcct
      121 gtttggaata gccagccttt aatggataac tatttctgac aatgcaatga gtataatcaa
      181 gtccatcttc cactgagaat tagaggcggc atgctttttc cggctcttgc cggatatccg
      241 taattgtcca taatctgcag atttatacct tctgcatgac ctgtcagcga aaatttgtcc
      301 ggtgtttcta cgggaatgac atcaaaagtt acacgcactc gcgttaccgt gtagacctac
      361 tttcctgcac ttgcaagatc acagtggtgt aaccgtaaca ggaatttatt ctctggaccg
      421 gcagtaaatc cctgagtggc gtggttccca tatcaatttc ccgccaggca gcctccattg
      481 ccagcgtaca ggctggagcc atgacctgcc ctttaaatct ggcccgacca tcccaccgga
      541 cgtgttcttc tcccctgaac ttaggtacag tcatctccag tggcacaaaa gtgtcagcgc
      601 cataattttt gaccgttatc gcgctacgga tattttgttg actggtgaaa atcaccccgc
      661 agaatcaggc ttattccctt aacctgggct ttcatcctga ccgccgcctc actacgacca
      721 atcagactgc cgatgcattt taccttcatt gttagtatca tgatttcagg cctgcaccat
      781 ccgctcattg cccggacttc cgacaaatcc cggcaaccat atcccggtgc ttgttcagct
      841 cccgcagcgc ggcgcagact cgctcccact tctggacatg attcttcgcc ctacgcagtt
      901 cgcgatttgc catatgcagc gatggcaaaa tcaaatcatc ttctcgcgtt gcagtaaacg
      961 atggcagcga ctgcacaatg tccgccacag tttctgtttt aatatcttcc tgtgttgcat
     1021 ctttctgtac cggtaacgca acaccggctg gctgaggaaa ggctttacca tcggtttccg
     1081 ctaccgattc aactttcggc tctgctggta aattatcacc tggtatgcag taacgaattt
     1141 taccgttctg gtttacgcga atcagacgac ctttgctgat tgccattgcc agcgttgaag
     1201 caactttgcg ggatgttgta ccgaacagcg tagccagttc atccgccgtt tgtggtccgc
     1261 gttgttcaat cgtcgcggtt aaatcgcact ctgagatttt cgcttctgtt gccgtggtgg
     1321 tttcttctgg cgctggctgt tcctgctgaa cgttgttatc agccacacgc caggtgtata
     1381 ctcttttatc aacgaagcca gcctttttca gttcccacag ctcgttcagt acttcttcac
     1441 gactgatatc aagtcgcgca gccagctcta ccgacgtggc ttttcccatc gctttcagtg
     1501 cgtcaaaaac agtctccata aatttcctcc cggtaaaaaa tcacttctca actcaaacaa
     1561 aaccagccgc tttccggcgt tcatattcct gtttcagcaa ctcaattggc gttggccccg
     1621 acgggcgttt gggggccgcc agttgtcgcc ggactggcgg aacgctcagg ccgttactaa
     1681 catgctttgc ccatttcgtc agttgccgtt ctgcaagccg ttttaattcc ccttcggtca
     1741 tctggcgctc aatccccttt gaacgcatct cgaggcaaat gtgatacagc acaggctgag
     1801 accacgggta cttatcactt ccgtcgtatc gccaggactc attgcgccag cggcggtact
     1861 cctccatcac agcatccacc gtcagaccga atggattggc tccgcttttc gaaatcagtg
     1921 ccacaaactc agccaggtcc ggaggccatg tttcacccgc ccggcagcgg tccatgcact
     1981 ggcggcagac ctgccggatt tgctgctcag tcatcgcgcc aatctgtgca atccagagct
     2041 tcgaaggtgc ggccccgttc ttctggatcc agcggttcga ataaaccacc cccatgagtt
     2101 cccacagctt ccaggccgtt tccgtcgctg ataaatccgt tttcacgttc ccactgctca
     2161 cgtgctgccc gaatttcctg aactgcccgt gatgcggtgc cacctggtgc tgctgcatgg
     2221 tttacccctt tgctgactgg tttaacctgc gccctgacgt gatttacgtg acgggcgaat
     2281 ttctgctccc actgaatctg cgtaaacact ttcccctccg ctgcccagta gtcccggaag
     2341 gcggcaagtt cagcaggtgt aaattctgtc tccggcaaag ccatccccca caacgcagcc
     2401 cgtcgtcgaa aatcccgtga cggataccag ctatcgctca tcgggaattt tccgatgggt
     2461 tcgctcaggc catacaggaa tacagggggg gctgcctgta acgacaaaac ttcctgctca
     2521 ctggtcggag cactctcgcg tgcgttatgt gtggggttta gatctttggg ttcctttggg
     2581 ttccgtgatc cgtttttggg tgtctttgat ggaaaatttg ggtgtctttg gttattttcc
     2641 atgcagctaa gagttctgtt tttgggtctg ttttgtgctg aaacataacc attttcggta
     2701 ctgtttttat taacagcacc aattttaccc acctttaaag actcccgttt ttgggtgtat
     2761 tcaggctcgg caacactttc ttctacaccg ataagtcggt acaccacaat ttgctttgtt
     2821 ctgccttttc tctcaccggt atcaacaatt aacccaatct ccatcaggtg tcgtaagctg
     2881 tcctgcacag tctttttgtt cagttccgtt acttctgcca gtgcagatac agacgggtat
     2941 gcacacaaat cggcaccgca catatcagca agccaggtca atactgactt actggatgaa
     3001 ctgccggttt tcaccttttt agcccatcgt agtgcatcga tactcatacg aacccctggc
     3061 agacatttgt ttatctgcaa agtaatattg gtattgctga cgatacgcgt gcttgaaagc
     3121 aatagctttt tctataagct cgtcagtctc acgttccaca acaactggat ccgcaaaaag
     3181 cagcccggac tccaccacat cgccatattc tttgtttaac ccggcgatca tgtacgtaat
     3241 gctttttccg tcagtaattt cacaatacaa tctgaaatcg ctgatccgga tagcctccat
     3301 aattgccgga atcagcgccg tgaatttgtc acgcttatct ctggtgtcga tagctttcca
     3361 gcgttcgaat atcttcaccc ggttaacgcc cagcgcccgt tgatcaacct cgccatcatt
     3421 aaacgtgacg cgttgaacat cgatgttcgg gcgttctttc agagcccaga atgcttccgt
     3481 gattaatatc gtcgcctgct cctgtgtcat tcctggtcgg catacccagg tatccagagc
     3541 ctcacaaacc tgttcagtgg tgattttcat tgttcaaccg ccccgcccgc tttgccttac
     3601 gatattcgtc ataaactttg gggtcgtact gaagttcccc gccggatgcc tcttgcaggc
     3661 gcatcgcgcg accttcagga accagttccc cccatgcagc aacgcttgcc agcctaactc
     3721 ctgcgacatt ggcaagcttt gttttgctgc caaaaacgct atagcatcaa ttttcaacat
     3781 atcgaacccc ttagattttc ctaaggaaac tagatcgtag agaaacctaa gtcaagaaaa
     3841 attagaatta cctaatatga aaaacgaaac cttcggtgct cgcctcttat acaggcgaaa
     3901 aaaattaaaa ctgtctcagg ccgcattagg taagctggtc aaagtggctc acgtaacaat
     3961 ttctcaatgg gaaagagatg aaacacagcc agcggggaag agattattcg cactgagcca
     4021 ggcgcttcag tgctcgccga cttggcttct ttttggagat gaagataagc aaccaggcga
     4081 accgatccca gataatcagc cagtcaatct gacagaagat caaaaagagt tgcttcaact
     4141 gttcgacgca ctgcctgagt cagagcaaaa ggctctgttg tcagagatgc gtgctcgagt
     4201 tgagaatttc aacaaacttt ttgaagaact actcaaagct cgcaaaagaa gcgcaaataa
     4261 ataacccccc tttttttcgc aactctctgt aataaaaagc acaaactttc aaatacttgt
     4321 gttttttaca tcaagaagct taggtttttc tacacaaaaa gcttgaccat aactcttagg
     4381 ctattctaaa ttctactcat caagacaccg cacggtgttc tcagcaaaca gttccgctac
     4441 ccggcgttaa ggggaagcag aggatttctc agtgggcgaa gtcaaacatc agaatggaag
     4501 gcgtccaggg atcagcaaag aaacagcgat ggcgctttat attgatatca gcgccattgc
     4561 cggacaggta agagttatca gagcggtaac taagcggtat gcgcctttac ttcagaaagt
     4621 ctctggtgag tgcaccgaag atattgtcaa cgatttcgtc atcgaactgc gaggactcat
     4681 cttcagttac aaggtgacca caatttttgc agatggctcc cgcgaaactg tcagagccct
     4741 gcggcttaaa ggatgtgtca aagacttagc caccacattc tgggcaagaa aacttgattg
     4801 tattcataac caatttcctc tcgagtaaca gacccctcag aggataccac ctcgcctgac
     4861 gtggttaaaa agcaggcaac gctaaccaca aggagccgac atgcagaaac gagcccccgt
     4921 catcatcgcg ccagactata ccgatgatga actttatgag tggatgcacc agaaaattaa
     4981 ggcggtgcag gacctgaaat gggccaatga agccagggct aagcaggctg aaaatctgtc
     5041 cgctctggag caggatatca ccaatctgga aaaagcagcg gcattaagca ttgccagaat
     5101 gattacatac ccacgttaat agctaaccaa cgaggctaat aatggaattt aaagatttac
     5161 caacgccatt acaggaaatg gcatcgaata tagttcgctc acaactggct actcttgacc
     5221 ttagtaccgc agaaaaagaa accatcgata atatggttcg taatgtgcgc aatgcttttt
     5281 ctgggctata tggttctgat aatcaaaagc aggaaagcga tgttaataaa cgggtaattt
     5341 ctgtttgcgt gaatggccat gttctttcat caatcaaaac agaaacggcg acagtcttcg
     5401 attgcctttg cattgtacag agccttgttg atgccctgtt tcgttcagtg aatttagaaa
     5461 atgatgcaaa tctgcgaggg cgcataatag cacatccata tgcacatact ttaggctctg
     5521 tggatatcaa agatcccaca aatctttaat gaaatagtta acgcgaattg tacttgctct
     5581 ttcggttgct ttcagaatac gcgttgaaac tgctggcggt aatttggtat tccatttatt
     5641 aaaatcatgc ccgggaaagt actcttcgaa aatactttta actgcagact cgcctattga
     5701 aatgctgctt accatgcgat tttgataaag gcatttagca ataagagttg attttaacat
     5761 tcaccctcct gagggttggt aattaaggag ttctccacgg gtgaagtgga gtgcgtgcgc
     5821 cggacacggg tgaacatccg gcactgacag tttactgaaa ggatatttct ctgaaaagtc
     5881 agaccataac gcgaaagcgc acggcgaggt agctggttca tagatagcct gtcgttaaat
     5941 tttcgtcgac cgtgcgcttc cggttgtggc aatccgcgaa atggcgcgtc ggtaagtatg
     6001 gaggggttat tccttccccg ttgaggacac cgggttgtca ggttgaccat acgcttaagt
     6061 gacaaccccg ctgcaacgcc ctctgttatc aattttctgg tgacgtttgg cggtatcagt
     6121 tttactccgt gactgctctg ccgccctttt taaagtgaat tttgtgatgt ggtgaatgcg
     6181 gctgagcgca cgcggaacag ttaaaaccaa aaacagtgtt atgggtggat tctctgtatc
     6241 cggcgttaat tgttaactag ttaacgtcac ctggaggcac caggcaccgc atcacaaaat
     6301 tcattgttga ggacgcgata atggaaacgt tattaccaaa cgttaatacg tctgaaggtt
     6361 gttttgatat tggtgttctg ctcagtaacc gggagtttac ggaagatgcc attaagatga
     6421 gaaaatatga accttatctt ctcaatgata attccatact ttccagaatt gcccttcttg
     6481 aacttggtat tatcggagaa cagcagtgac ttcagcattt gcactggtga tgaccgtttt
     6541 tcttataacg ggtgagccac aaaatgtgat taccggaatt tatgacagta agtcatcctg
     6601 cattcaggta agggacgaac aaaaaatccc cggtgaatgc ctcccgttaa aaaaagtatc
     6661 gctgaacctg aataacgaaa taccggctgg ataacccgcc agccatatta acgccatacc
     6721 aacggattaa aaatgccagc aatggcaggg attcgttcac cctgaaatct gtaatgaggt
     6781 taaaacaaaa tgagtaaagt ctttatttgc gccgccattc cggacgaaca ggcaataaag
     6841 gaagaaggtg ccgtcgctgt agccactgcc attgaagccg gtgatgaacg tcgcgcccgc
     6901 gcaaaatttc actggcaatt cctggaacat tatccggctg ctcaggactg cgcttataaa
     6961 tttcttgtct gcgaggataa acctggtata ccccgccctg ccctcgattc ctgggatgct
     7021 gaatatatgc aggaaaaccg ctgggatgag gaatccgctt cctttattcc ggtcgaacca
     7081 gaatccgatc cgatgaacgt caattttgac aagctgtccc
//
LOCUS       DAZGTR010000188         7106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGTR010000188 DAZGTR010000000
VERSION     DAZGTR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.190"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            148..1482
                     /gene="adeQ"
                     /locus_tag="ACHLCY_004553"
                     /gene_synonym="yicO"
     CDS             148..1482
                     /gene="adeQ"
                     /locus_tag="ACHLCY_004553"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP0371651.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1535..1987
                     /gene="yicN"
                     /locus_tag="ACHLCY_004554"
     CDS             1535..1987
                     /gene="yicN"
                     /locus_tag="ACHLCY_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP0371652.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2198..3388
                     /gene="nepI"
                     /locus_tag="ACHLCY_004555"
     CDS             2198..3388
                     /gene="nepI"
                     /locus_tag="ACHLCY_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP0371653.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQITFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAILWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3429..3722)
                     /gene="yicS"
                     /locus_tag="ACHLCY_004556"
     CDS             complement(3429..3722)
                     /gene="yicS"
                     /locus_tag="ACHLCY_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP0371654.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQNNYWEALGKVECPDM"
     gene            3944..4762
                     /gene="nlpA"
                     /locus_tag="ACHLCY_004557"
     CDS             3944..4762
                     /gene="nlpA"
                     /locus_tag="ACHLCY_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP0371655.1"
                     /translation="MKLTTHHLRRGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4766..5689)
                     /gene="yicL"
                     /locus_tag="ACHLCY_004558"
     CDS             complement(4766..5689)
                     /gene="yicL"
                     /locus_tag="ACHLCY_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP0371656.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSNALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5800..6984)
                     /gene="setC"
                     /locus_tag="ACHLCY_004559"
     CDS             complement(5800..6984)
                     /gene="setC"
                     /locus_tag="ACHLCY_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFP0371657.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLGDE
                     LKARPIMVDFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1787 a         1596 c         1787 g         1936 t
ORIGIN      
        1 tgattattat ttatcataaa gaaacgtcgc gagattatta aagaatatcc attaatgtgc
       61 aattgaaatg tgataattat cactgaaccg ttgttgtcat ttgttttaca aaagcaaggg
      121 attaaatctc ctccaatgga caaaaaaatg aataatgaca ataccgatta cgtgagtaat
      181 gaatcaggga cgctttcgcg attatttaaa ctacctcagc atgggaccac cgtccgcaca
      241 gaattgattg cggggatgac cactttttta accatggtgt acatcgtttt tgtgaacccg
      301 caaatcctcg gcgcggcaca aatggacccg aaagtggtgt ttgttaccac ctgtttgatt
      361 gccggtatcg gcagtattgc gatggggata tttgctaact tacccgtggc gctggctccg
      421 gcaatggggc tgaacgcctt ctttgccttc gtggtcgtgg gggcgatggg catctcctgg
      481 cagaccggga tgggcgcaat attctggggc gcagttggac tatttttgct cacgctgttt
      541 cgtatccggt actggatgat ctccaacatt cccttaagtt tacgtattgg tatcaccagc
      601 ggaattggat tatttatcgc cttaatggga ttaaaaaata ctggcgttat tgtcgccaat
      661 aaagacacgc tggtgatgat tggcgattta agttctcacg gcgtgttgtt aggtatttta
      721 gggtttttta ttataaccgt gttgtcatca cgtcattttc atgccgcggt gctggtttct
      781 attgtggtga cgtcttgctg tggattattt ttcggtgatg ttcattttag cggcgtctat
      841 tccattccgc ctgatattag cggcgtcatt ggtgaagtag atttgagcgg cgcgttaaca
      901 cttgaactcg ccggtatcat tttctccttt atgctgatca acctatttga ttcatcagga
      961 acattaattg gtgtaactga taaagcgggc ttaatagata gtaacggtaa attccccaat
     1021 atgaataagg cgctgtatgt tgatagcgtc agttcggtgg cgggggcgtt tatcggcacc
     1081 tcgtctgtta ctgcctatat tgaaagtact tctggtgtgg cagtcggtgg tcgcacgggg
     1141 ctgactgcgg ttgtggttgg cgttatgttc ctgttagtta tgttcttctc accgctggtg
     1201 gcgatggttc ctccttacgc aaccgccgga gcgttaatct ttgttggcgt gctgatgact
     1261 tcgagcctgg cgcgcgttaa ctgggatgat tttaccgaat cggtgcctgc gtttattacc
     1321 acggtgatga tgccctttac tttctcgatc accgaaggga ttgcactcgg ctttatgtcg
     1381 tactgcatca tgaaagtatg caccgggcgc tggcgcgatc tgaacctgtg tgtggtggtg
     1441 gtcgcagctc tgtttgcact gaagattatt ctggtggatt agtaactggc gagggtttcc
     1501 agatatcatg agttctgatt acgcaggaga actcatgatc tggataatgc tcgccacgct
     1561 ggcggtagtg tttgtggttg gttttcgggt gctgacatcc ggggccagaa aagcgattcg
     1621 ccgtctcagc gatcgactga acatcgatgt cgtacccgtg gagtcgatgg tcgatcaaat
     1681 gggaaagtca gctggtgacg aatttttacg ttatttgcat cgtccggatg agtcgcacct
     1741 gcaaaacgcc gcgcaggtgt tgctcatctg gcaaattgtc attgtcgatg gtagcgaaca
     1801 gaacctgctg caatggcatc ggattttaca aaaagctcgc ctcgccgcgc cgattaccga
     1861 cgctcaggtc aggctggcgc taggttttct gcgcgaaacc gaacctgaaa tgcaggatat
     1921 taatgctttt cagatgcgct ataacgcgtt ctttcagcct gccgagggcg ttcactggtt
     1981 gcattgaaac cgtttcgtct tatcagccta cagaccgcgc ctgacctgtc gggtggataa
     2041 gacgttcacg ccgcatccgg taatctttgc cctgttccgt ttcgatctta actccagctc
     2101 aagcaacacc gtaaaagttg caaaaacgtt aaatgcgtca cacatttcaa tgttaaagtg
     2161 gtcggctttt cccctgaaac atgccacggg taacaccatg agtgaattta ttgccgaaaa
     2221 ccgcggcgcg gatgccatca cccgaccgaa ctggtcagcc gttttctcgg tggcgttttg
     2281 tgtcgcctgt ctgattatcg ttgagttttt gcccgtcagt ttgttgacgc caatggccca
     2341 ggatttaggc atttcggaag gggttgccgg gcaatcggtg accgtgaccg cctttgtggc
     2401 aatgtttgcc agtttgttta ttacccagac aattcaggct actgaccgcc gctacgttgt
     2461 tattttgttt gccgttttgc tgacgctctc ctgcttgctg gtttcctttg ctaactcatt
     2521 cagtttgctt ttaatcggtc gtgcctgtct ggggctggcg ctgggcgggt tctgggcgat
     2581 gtcggcgtcg ctgaccatgc gtctggtgcc gccgcgtacg gtgccgaagg cgctgtcggt
     2641 gatcttcggc gcggtttcta ttgcgctggt gattgccgcg ccgttgggca gttttttagg
     2701 cgagcttatc ggttggcgca atgtctttaa tgcggcggcg gtgatgggcg tgctgtgtat
     2761 tttctggatt atcaaatcat tgccttcact gccaggcgaa ccctcgcatc agaaacaaat
     2821 tactttccgc ttattacaac gtccgggtgt gatggcaggg atgatcgcca tcttcatgtc
     2881 tttcgccggg cagtttgctt tcttcacata tattcgcccg gtgtatatga acctggcggg
     2941 attcagcgtg gatggcttaa cgctggtgct gttgagtttt ggcattgcca gctttatcgg
     3001 tacgtcgctt tcgtcattca ttcttaaacg ttcggtaaaa ctggccttag caggcgcgcc
     3061 gttaatactg gctgtgagtg cgttggtgct gacgttgtgg ggaagcgata aaatcgttgc
     3121 taccggcgtg gcgattctct gggggctaac ttttgcattg gttcccgtcg gctggtcaac
     3181 gtggatcacc cgctcgctgg ccgatcaggc agaaaaagcc gggtctattc aggtggcggt
     3241 tattcagctt gctaatacct gtggcgcggc aatcggcggt tatgcgctgg ataatattgg
     3301 tctgacttcg ccgttgatgt tgtccggcac attgatgttg ctgactgcat tgttggttac
     3361 tgcaaaggtg aaaatgaaga aatcctgata tcaggtaaag aagcgttggt cagtagttgt
     3421 tgctgttctt acatatccgg gcattctacc ttacccaacg cttcccaata attgttctga
     3481 ttattacgtt ccatcgccac gatggcctcg cgtatatgtt gtttattgtt ttcatctgac
     3541 aacattaact tttcccacgc ttcatctgat aatgacgcct gtggcgtgca aattttacgt
     3601 aaatcttgta ataccgtggt tttctctttt gcggactgca gtgagctaaa aggcgactcg
     3661 gcgtatacga tgcctggcat agcgaaaaaa gtgaaaataa gcagtagtgt cgttggtttc
     3721 atggattcaa ccttttacaa atgtgtatcg aatgaagcat aagtgtagtt tgctttcatc
     3781 gctctcatca tgctgtacga aacgcgcttg cgtggtcagt aagaagtgcc agactttata
     3841 ttccactttt attccttttt attcttatcg atagcgtttc gttttttaaa ccgcagcgac
     3901 cttaccgcta tagtcaggta atcattaata aaaggataaa aaaatgaaac tgacaacaca
     3961 tcatctacgg agaggggccg cattattgct ggccggaatt ctgctggcag gttgcgacca
     4021 gagtagcagc gatgcaaaac acattaaagt tggcgttata aatggcgcag aacaagatgt
     4081 cgcggaagtc gctaaaaagg tggcaaaaga gaaatatggc ctcgatgttg aactggtagg
     4141 atttagcggt tcgttgctac cgaacgatgc gacaaatcat ggcgaacttg atgcgaatgt
     4201 cttccagcat cgaccctttc ttgaacaaga taatcaggcg catggttata aactggtagc
     4261 ggtgggaaat acgtttgtct tccctatggc gggttattcc aaaaaaatca aaaccgtggc
     4321 gcaaataaaa gaaggcgcga cagtggcgat ccccaacgac ccgaccaacc ttggtcgcgc
     4381 acttttactg ttgcaaaaag agaagctaat tactctgaaa gagggaaaag ggttattacc
     4441 taccgcgctg gatataaccg ataatcctcg tcatttgcag attatggaac tcgagggggc
     4501 gcagctcccg cgcgtactgg atgatcccaa agttgatgta gcgattatca gcaccactta
     4561 cattcagcag acagggcttt ctccggtgca cgacagcgta tttattgaag ataagaattc
     4621 gccgtatgtg aatattttgg tggcacggga agataataag aatgcagaaa acgtgaagga
     4681 atttctgcaa tcttatcaat cacccgaagt cgctaaagcg gcagaaacca tttttaacgg
     4741 tggcgcggtg cctggctggt aattctcact catgccgcgc cggacgattt atttttctgg
     4801 cgcggcgacg ggaatccatt gaaatcaaaa ttaccgatga cagaattagc agcgttccca
     4861 gccagtccgg taatgtgaac gtgatcccca gcaacagcaa agagagtagc gcgttactta
     4921 atggttctgc acagctcaaa atgctggctt ttggaccgcc aattaattgt gctcctttca
     4981 ggtacagact aaatgtcagg gacgtaccaa tgacgatcaa ataaaaaaac gccagaatca
     5041 aactgccgtt aacgacaaag tttgttcctt gtctggcata aaaaggcaac agaatcagac
     5101 cgccaatcag catactccag ccgacgactg gtaacgtgcc atagcgggca attagcgttg
     5161 agggataggt ggtatagaat gcagcagcaa atgccgaggc aatgccccag aacaacgcgg
     5221 caggagagat cgataatgac gtcggattac cgtgtgtcac caataaaaaa gtcccgacca
     5281 gcgatgtcaa aatagcgcag aaaaccagaa tgcccgggcg cgatttacgc accagtgaga
     5341 accaggcgac gataatcgtc ggtgagagga attgcagcac cgttgccgtg gctgcgttcg
     5401 atttttcgat ggttagcaaa aaagtgagct gtacagttag cgcgccaacc acggaaaaaa
     5461 tcagcaggct aatggcatct ttatgattgt taataataga aaagatttta tcgccatgaa
     5521 caaatgacag cgtcagtaga atcaaaccgg cgaatatcaa acgcgtcata gtcaaaaact
     5581 gcgacgacat ctggctttgc tccatgatgt attgcgcaca gacccctgaa cttccccaca
     5641 acacggcggc aatcagaacg ttcagcatcc cctttctggt ggaacccatt tttccctctg
     5701 catactgttt tgtcgtttat gtatgcaggc atgatagcaa aaatggcgag gatggctctg
     5761 tttgcgccac gccgattttt cttagcccag actggtgagc taaatatctt taataaacag
     5821 caggcaaatc atcgcaatac ccaacatccc tatcgccagc cagaacaacg catggtagct
     5881 ccagatttca accataattc cgtcaacaga gccggcgata atccagccga cgcgtgaagt
     5941 atttgcatat aacgttgtcg cagagcctat tttttcaggc atcaagtcct gaaaataaag
     6001 catgccgata ccacagagaa taccaaggaa gatggcatta agaatttgca attccagctc
     6061 aaccgccgga gtcgtcgcca tgagtacgct ggcgtaaaaa cacattccac tcacgatagc
     6121 aatgagcatt aatagtcgct tgccaatacg tttcatgtaa tagcctgcga ttaacatcat
     6181 cggaatttcc agaccggcag cgataccaat catttctcca gccagtttat cggttagatg
     6241 cagttcatca ataataaata gcggcatatt tatcatgtag agattattcg ccgcccacat
     6301 cattgaacag accacaaaaa gtagccgcgt atcccgcttc ctgtgggtgg agggtaaaat
     6361 ttctacgggt tgggtaacga caggaatatt tctttgtatt gatggcaaaa acaaccagac
     6421 tatcagtccg caaacaacaa atgcgatggc agcggtgaga tacatcactt taaaactaaa
     6481 tcccattgcc agttcataag cgagcggtgg cccgataacc caggcaagcg agatctgagc
     6541 acgtaaaaat gtactgaaca tgaccgtctc acggcctgtt ctgtcggcgt gttcacgggc
     6601 gagggcgaac atttgcgggt ttgcggtgga agcaaaacta ctcagaagta cgcccgttga
     6661 gaggagaatg aagtagttgc gattccacgc aaaaagcgtg caggccagca ctccaaataa
     6721 gcaacatagc agaatcagta atttacggtc gccttgttta tcggagtgcc ttgccagaaa
     6781 ttgactgacc agaattccca taatagcgct accggtgaag aaaaaatcta ccattatagg
     6841 acgggctttc agttcatctc cgaggaatat acttagggta ggagtctgaa gagcgcccgc
     6901 aatacccgtc agaaaggcga caagtaaaaa tgccgcggca gtgagatcaa gtatttttga
     6961 tggagtggta gccgtttttt gcattcttct gaatcaccgg gcgacaaaat ataactgagt
     7021 tacaaataag taccagtcga atgagtattg tattaagaat ttttgagtat cgctgataca
     7081 accagaaagc aatgctagcg caatat
//
LOCUS       DAZGTR010000189         6912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGTR010000189 DAZGTR010000000
VERSION     DAZGTR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.191"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(193..678)
                     /gene="sixA"
                     /locus_tag="ACHLCY_004560"
     CDS             complement(193..678)
                     /gene="sixA"
                     /locus_tag="ACHLCY_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP0371658.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(881..3025)
                     /gene="fadJ"
                     /locus_tag="ACHLCY_004561"
     CDS             complement(881..3025)
                     /gene="fadJ"
                     /locus_tag="ACHLCY_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP0371659.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3025..4335)
                     /gene="fadI"
                     /locus_tag="ACHLCY_004562"
     CDS             complement(3025..4335)
                     /gene="fadI"
                     /locus_tag="ACHLCY_004562"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP0371660.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4515..4799)
                     /locus_tag="ACHLCY_004563"
     CDS             complement(4515..4799)
                     /locus_tag="ACHLCY_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP0371661.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5171..6511
                     /gene="fadL"
                     /locus_tag="ACHLCY_004564"
     CDS             5171..6511
                     /gene="fadL"
                     /locus_tag="ACHLCY_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP0371662.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1733 a         1847 c         1631 g         1701 t
ORIGIN      
        1 attattcatt tcaacatgta actttgggca aaatcaagat acgcgcagaa gaataatttc
       61 cgaatgaaag tagaaagtgt tttatttata gatggggaga gtcttcgata gtagtgaggt
      121 tgacagatat ccgctgcctg atgagcaatt atgcccaatg aatctacctc attgggcata
      181 atttggaact catcagatag cttttgccat cttcagatta cacgggctca tctgccagtt
      241 gaatgtgccg tttccgcttt catcaagcgt tacgctggca atggccgagg tggtaaacat
      301 aggcggcgtt tcgcccggac ataactcggc aaccagatac ccgactaacg gtaagtgaga
      361 gatcaccagt accgaagcaa caccttcatt ggtcaacgcc tgcaaatagg cactgaccag
      421 accaacatcg ccgcagggcg ttaactccgg cagaacttct gcactggaag gcaggttcag
      481 acaatcccct acttcttcca gtgtttgctc ggctcgcagg aacgggctca ccagaacgcg
      541 ttcgatttcc actttttgac ctttcagcca gttcgccatc aggcgagatt cgtcacaacc
      601 attagtggtc agaggacgaa cggaatcact ggcggcatcg agggctgcgt cgccgtgacg
      661 catgataaaa acttgcatat tgcaccgctt ttgttaacca gattcgcccg ttttctttca
      721 tctgaaacca gagaaagaaa acggtggccg gcattgtgcc ttatccattc accgaatgaa
      781 acgctgtttt ttacctcaat ggcgtaagta tagtcaatat gcgtttacat tttgaccaac
      841 actccgccat tcagcgcgga ttcatatagc tttgaccttc ttattgcagg tcagttgcag
      901 ttgttttcca aaaactttcc ccacgcgcgc ccatctcgac caaacgctcg caaggggtaa
      961 aacgggaacc atactgcgtg gcaagtcgtt gcattattgc aaccacttcg cccgcgccga
     1021 gagaatcgat atagcggaac ggtccaccga gaaatggcgg aaaaccaatg ccaaataccg
     1081 caccaatatc cccgtcacgc acgctacgga taacctgctc atcaacacaa cgtactgctt
     1141 cattcagcat caacatcaca caccgttcag caacctgcgg tgcggagagt cgcccctgcc
     1201 cttgtgcgcc aatgagcggg taaatggcgg gatcgacctg ttttttgctt ttacgccctt
     1261 tctgaccata aagatagaaa ccccgaccat tttttctgcc tttgcgatcg tcgttcaaaa
     1321 ttgaagaaac aacatttgca ggcgcgctaa aacgttctcc ataagcggct tccagtacag
     1381 gaataatttt agtcccggtg tcgattccta cctcatccaa aagttggatt ggacctaccg
     1441 gaaaaccaaa tttcactagc gcggcatcaa tgtgctctac ccgttcacct tcggtcagca
     1501 tgcggatagc ttcattaatg taaggcgcta agatgcgatt gacgtaaaac ccggctttgt
     1561 cacgcacgac aatgggcgtt ttgccctgtt ttttcgccag ttttaccgta gtggcgatgg
     1621 tttgcgccga tgtacccgca tgaggaataa tctctaccag cggcattttt tccaccggac
     1681 tgaagaaatg caggccaata acttgctcag gtcgcgtggc gtgagctgcg atatcaccaa
     1741 tcggtaaaga tgacgtattc gaagcaaaaa tggtatgagc ggcgcaattt tgctccactt
     1801 ccgccaccat ctgttgtttt aattcgagat tttcaaacac cgcttcaata atcagatcgc
     1861 gatgggcaaa gccgcgatag tccgtcgttc cggagattaa tgccagctgt ttgtcacgtt
     1921 cgctggcttt gagatgacga cggcgaactt tgccctccag ctgatcccaa ctgtacttca
     1981 gcgcatgatt tatgccctgc gggttgatat ctttaattct gaccggaagc cccgctttac
     2041 aagcagtgac ataagcaata ccgccgccca tcaagccacc acctaaaatt cccacgctgt
     2101 ttaatggcgc aggcggtgca tcactaccgg gatctttctt cacttccgta ctggcaaaaa
     2161 agatactacg caaagcttgc gattgtggcg tcatcgccag ttcgccaaac gcccgagctt
     2221 cggcgtcata accgctgctg gtgccctgcg ctaatcccgt ttcaacaacc tccaggatgc
     2281 gttctgtcgc cggataattg ccttgagttt tgtgttctgt tttcttgccg accattttga
     2341 acagcagcgc acgacctaac ggccccgcca gaatacgctc gcgtacaggt agagggcggg
     2401 aagatgggcg atccttcttc gccagctcaa cagcgacttc cagcaaaatg gagtgcggaa
     2461 caacgtcatc caccagcccc agctttaatg cctgtttcgc ccgaagttgt tttccggtga
     2521 ggatcatctc taatgctgtg ctgacgccta tcagacgcgg taaacgctgg gtgccgcctg
     2581 aaccgggtaa caatccaagt tgtacttcag gcaaaccgag caccgtttta ggatcgtcag
     2641 tacaaacgcg accgtggcac gccagcgcca actccagccc accacccagg caagcgccat
     2701 gaatagccgc gataaccgga atgggcaaag catgaatctc cgccatcaac tgttggccct
     2761 gccgcgccag tgcttccgct tcttgcgccg ttttgcagtt gccgatcatg ttgatgtctg
     2821 cgccagcaat gaagttgtcc ggtttagcgg agacaaacac cacgcctcgc aactctttgt
     2881 tttcacggag ttgcttaata atggcgcgca cctgcgaggc aaactccgcc ttcagggtat
     2941 tcattttctc acccggtacg tcgatggtga taacggcaat gttgtccaga cgaacattaa
     3001 gggtaaacgc tgatgtcatt tccattattc cgcctccaga accattgccg cgccaagccc
     3061 accggcagca caggcggtaa ctaaaccaaa tccaccgccg cgacggcgaa gttcatgcaa
     3121 tgtctgggta atcatccgtg cgccggtcgc cgcgaaggga tgtccgtaag caatcgaacc
     3181 gccaagcacg ttaaatttgc tatcgtccac ttcgccagtg gcatgtgcac gccccagtac
     3241 ttcacgagca aaacgttcac tacccagcaa ctgaatattc gccagtgtct gagctgcaaa
     3301 ggcttcgtgc atatcgatca atgtcagatc gctcattgtc aaaccggcac gttccagcgc
     3361 cagcggtgtt gaccaggccg gaccgagcaa catgtcctgc cagacatcaa tcgcagtaaa
     3421 tgcgtagctg cgcagatacc ccagcggcac cagccctaat tctttcgccc gggattcagt
     3481 catcaggatc accgctgccg cgccatcggt cagcggcgta ctgtttgccg ccgttaccgt
     3541 tccgtgtttg cgatcaaacg ccgggcgcag ctttgcgtaa tcggcaagcg aggaattacc
     3601 gcgaatattg ttgtcttcga caagcggttg tttataagga gggataaagg cagtcatcac
     3661 ctcttctttg agttttccgt ctgaccatgc ctgagcggca cgctgatgcg aacggtgcgc
     3721 taatgcatct tgctgttctc gggtgatgcc gtaggttttc gccatttgtt cggcggtgtc
     3781 gcccatccgc aagccagtag aatattctgc taccgcaggc ggtacgggca ttaagtcgcg
     3841 caaacgcagg cgagagaaga gtttcagtcg ctggctcatg gttcgagctt tgttgacatc
     3901 aaccagcacg cgcgccagtt ttttactgac gccaattggc aatacggaag aggaatctgc
     3961 cccaccggca atccccgctc ggatagttcc cgccatcagg ctttctgcga cgtttgcaac
     4021 cgcctggaaa ctggtagcgc aagcgcggct gacgctgtaa gcatcggtat gcacattcat
     4081 tccggtaccg agaacaattt cacgcgcaat gttgggggct tcaggcattt gtacgacctg
     4141 accaaagacc agctgttcaa tcacttcagc ggggatctcg ctgcgtgcca gcagttcgcc
     4201 taccaccatc ttccctaaat caaccgcggg aatgccatga aaagccgtcg cctggcgggc
     4261 aaaaggcgta cgtaaaccgc taacaatggc gatacgatcg ccctggcggg taaccagcgg
     4321 taaaacctga cccataacac tcccctgtaa aaaaataaat aaagtggtct gacctgatca
     4381 tagtcttaac cattttttta catttagcca agcggagaaa aaggaaagtg ggagctatga
     4441 cacagtgaag acaggagaaa gaaaagaaaa cgcccctgcc agcaactgac aggggcgtac
     4501 gtacccgaga ggaattaacg cagacccagc tggaagatca gcatttcagc ttcgcaggca
     4561 aaagtgaaat cgatatccag gcgcacaccg tcagactctt cagtgaaagt cggggtgatt
     4621 ttacacggtt cggattccac gctacgggct ttttcagtca gcgccgccag cgtttgttct
     4681 gcttctgcgc ggcttgcaaa cacgcggctg taagacgcgg tgcagtcgga gttgtccata
     4741 atggtgccaa catccataca gcagcaaacc ggggtttcat cagcactaca tttactcatc
     4801 gttgatttcc tctgtatgtg cacccaaggt gccagataaa cgttgtggat attttacgct
     4861 tccggaaagt gctgctccag ttgttaattc tgcaaaatcg gataagtgac cgaaatcaca
     4921 cttaaaaatg atctaaaaca aaattcaccc gaatccgcga gtgcgcaacc tccaaatttt
     4981 gccagctgga tcgcgtttct tagatcatat ttgaaaaaag atggaaacat acttgcaaca
     5041 ttccagctgg tccgacctat actctcgcca ctggtctgat ttctaagatg tacctcagac
     5101 cctacacttc gcgctcctgt tacagcacgt aacatagttt gtataaaaat aaatcattga
     5161 ggttatggtc atgagccaga aaaccctgtt tacaaagtct gctctcgcag tcgcagtggc
     5221 acttatctcc acccaggcct ggtcggcagg ctttcagtta aacgaatttt cttcctctgg
     5281 cctgggccgg gcttattcag gggaaggcgc aattgccgat gatgcaggta acgttagccg
     5341 taaccccgca ttgattacca tgtttgaccg cccgacattt tctgcgggtg cggtttatat
     5401 tgacccggat gtgaatatca gcggaacgtc tccgtctggt cgtagcctga aagccgataa
     5461 catcgcgcct acggcatggg ttccgaacat gcactttgtt gcaccgatta acgaccaatt
     5521 tggttggggc gcttctatta cctctaacta tggtctggca acagagttta atgatactta
     5581 tgcaggcggc tctgtcgggg gtacaactga ccttgaaacc atgaacctga acttaagcgg
     5641 tgcgtatcgc ttaaataatg catggagctt tggtcttggt ttcaacgccg tctacgctcg
     5701 cgcgaaaatt gaacgtttcg caggcgatct ggggcagttg gttgctggtc agattatgca
     5761 atctcctgcc gggcaaactc ctcaagggca agcattggca gctaccgcca acggtatcga
     5821 cagtaatacc aaaatcgctc atctgaacgg taaccagtgg ggctttggct ggaacgccgg
     5881 aatcctgtat gaactggata aaaataaccg ctatgcactg acctaccgtt ctgaagtgaa
     5941 aattgacttc aaaggtaact acagcagcga tcttaatcgt gcgtttaata actacggttt
     6001 gccaattcct accgcgacag gtggcgcaac gcaatcgggt tatctgacgc tgaacctgcc
     6061 tgaaatgtgg gaagtgtcag gttataaccg tgttgatcca cagtgggcga ttcactatag
     6121 cttggcttac accagctgga gtcagttcca gcagctgaaa gcgacctcaa ccagtggcga
     6181 cacgctgttc cagaaacatg aaggctttaa agatgcttac cgcatcgcgt tgggtaccac
     6241 ttattactac gatgataact ggaccttccg taccggtatc gcctttgatg acagcccagt
     6301 tccggctcag aatcgttcta tctccattcc ggaccaggac cgtttctggc tgagtgcagg
     6361 tacgacttac gcatttaata aagatgcttc agtcgacgtt ggtgtttctt atatgcacgg
     6421 tcagagcgtg aaaattaacg aaggcccata ccagttcgag tctgaaggta aagcctggct
     6481 gttcggtact aactttaact acgcgttctg ataacgcgtt cgcctggata aagtcacctg
     6541 catagcaggt gactttaact cccccacttc accgaaagta gtgtcctcat tgcttacctc
     6601 ctgagttttg caaacagcct gatggcagct tgccctttca aatcaataag cggtatccac
     6661 agacaaatct gaagggaaag gcatattttc aggcgttctg ctcgtccttc tcaaagagtt
     6721 tacttttctg aatttccagg atactcaccc ccccctggct attgtgcgct catacactca
     6781 aattaaagat aggttctaaa taaatgagcg ttttttgata gtctatttca ttaggtaata
     6841 tatatttgta acaaatcaat caaaatggaa taaaatcatg ctaccatcta tttcaatcaa
     6901 caataccagc gc
//
LOCUS       DAZGTR010000190         6827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGTR010000190 DAZGTR010000000
VERSION     DAZGTR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.192"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            128..1231
                     /gene="proB"
                     /locus_tag="ACHLCY_004565"
     CDS             128..1231
                     /gene="proB"
                     /locus_tag="ACHLCY_004565"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP0371663.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1243..2496
                     /gene="proA"
                     /locus_tag="ACHLCY_004566"
     CDS             1243..2496
                     /gene="proA"
                     /locus_tag="ACHLCY_004566"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP0371664.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCRDQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2611..2686
                     /locus_tag="ACHLCY_004567"
     tRNA            2611..2686
                     /locus_tag="ACHLCY_004567"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2644..2646,aa:Thr,seq:cgt)
     gene            2852..4066
                     /locus_tag="ACHLCY_004568"
     CDS             2852..4066
                     /locus_tag="ACHLCY_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0371665.1"
                     /translation="MKLNARQIDTAKPKEKAYKLADGGGLYLLVKPNGGKYWRLKYRV
                     AGKEKLLALGVYPEVTLADARAKREDAKRGIAGGIDPMEAKREEKIARETQLNNTFKD
                     IALEWHSSKLKKWSAGYASDILEAFNKDVFPYIGKKPIAEIKPLELLNVLRRIEGRGA
                     TEKAKKVRQRCGEVFRYAIVTGRAEYNPAPDLTSAMQGHESNHYPFLTAKELPDFFKA
                     LSSYSGSALVVMAARLLIITGLRTGELRGALWDEIDFNKAIWEIPASRMKMRRPHIVP
                     LSDQALSLIGKIREITGNYPLMFPGRNDPRKTMSEASINQVFKRIGYAGRVTGHGFRH
                     TMSTILHEQGYNTAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDALEKE
                     ENVVHGSFGKRA"
     gene            4209..5090
                     /locus_tag="ACHLCY_004569"
     CDS             4209..5090
                     /locus_tag="ACHLCY_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6387 family protein"
                     /protein_id="HFP0371666.1"
                     /translation="MVRAKKQDLAWFDISNYEFLNDLTLPDLIQELEWRDFLLRHAKD
                     DTAIFKEEYDIKYERIFSGDPNLTILNEEEEEVEEFIRKVNDEAPSLRNEYDDLPSLS
                     SAMGVSPVTFSELAMYSFSSIDQGFFKRDEEDCYLKANAMLASVTGNLSNCSPNIILV
                     SIDLDDATDDEIITSLTHLLPLWRKELKVPEREHVAQKRIGLKTLQKLISNRVIPIVD
                     LLIWGEKSGKEVSNPMISALVFSDDPKDTQAIKESIKPFALESISERYTRLLQLYVNK
                     DREMSLIKISDLMSRDL"
     gene            5288..5485
                     /locus_tag="ACHLCY_004570"
     CDS             5288..5485
                     /locus_tag="ACHLCY_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015462155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0371667.1"
                     /translation="MSQSFIRLSEVQRRTGYSKAWIYRLIKEQRFPQSVKIGSRSIAF
                     IESEIEEWINQRIAESRGEVA"
     gene            5485..5916
                     /locus_tag="ACHLCY_004571"
     CDS             5485..5916
                     /locus_tag="ACHLCY_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371668.1"
                     /translation="MNKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYATELERQLGITLERIDEKNPDGIGTHLRYRFSCR
                     GDVLKVITHINHLANINDHNGLSQQEIADILKLYPDAFNAA"
     gene            5929..6762
                     /locus_tag="ACHLCY_004572"
     CDS             5929..6762
                     /locus_tag="ACHLCY_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFP0371669.1"
                     /translation="MNIEKSRLISEAAPHLNASLSTINGNEFAAIVPVIPGHIGGRET
                     NIVSAKALHKALGVGKDFSTWITDRISEYDFTIGHDYSVHKTISPNLGKSPNGVAYRK
                     IKQSGRPGKDYLLSVGMAKELAMIERNDQGRAIRRYFIQCEEELQRSVPEIAARYLRQ
                     LKARISAANNFKPMCDALNMARAEQGKTTQQHHYTNESNMISRIVLGGLTAKQWARIN
                     GYSGEPRDHMNAEQLEHLSYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVVH
                     V"
BASE COUNT         1856 a         1582 c         1739 g         1650 t
ORIGIN      
        1 ccgctaggct tgctgatccc gcgcaacaaa acgccatgct ttgctcgcag atggttggca
       61 accgacgaca gtcctgctaa aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca
      121 gagaatcatg agtgacagcc agacgctggt ggtaaaactc ggcaccagtg tgctaacagg
      181 cggatcgcgc cgtctgaacc gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt
      241 acatgccgcc gggcatcgga ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga
      301 gcacctgggt tacccggaac tgccagcgac tatcgcctcg aaacaactgc tggcagcggt
      361 agggcagagt cgactgattc aactgtggga acagctgttt tcgatttatg gcattcacgt
      421 cgggcaaatg ctgctgaccc gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg
      481 cgacaccctg cgtgcgttgc tcgataacaa tatcgttccg gtaatcaatg agaacgatgc
      541 tgtcgctacg gcagagatta aagtcggcga taacgataac ctttctgcgc tggcggcgat
      601 tctggcgggt gccgataaac tgttgctgct gaccgatcaa aaaggtttgt ataccgctga
      661 cccgcgcagc aatccgcagg cagaactgat taaagatgtt tacggcattg atgacgcact
      721 gcgcgcgatt gccggtgaca gcgtttcagg cctcggaact ggcggcatga gtaccaaatt
      781 gcaggctgct gatgtggctt gccgtgcggg tatcgacacc attattgccg cgggcagcaa
      841 gccgggcgtt attggtgatg tgatggaagg catttccgtc ggtacgctgt tccatgccca
      901 ggcgactccg cttgaaaacc gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat
      961 cacggtagat gaaggggcaa ctgccgccat tcttgaacgc ggcagctccc tgttgccgaa
     1021 aggcattaaa agcgtgactg gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct
     1081 cgaaggtcgc gatatcgccc acggcgtcag tcgttacaac agcgatgcat tacgccgtat
     1141 tgccgggcac cactcgcaag aaattgatgc aatactggga tatgaatacg gcccggttgc
     1201 cgttcaccgt gatgacatga ttacccgtta aggagcaggc tgatgctgga acaaatgggc
     1261 attgccgcga agcaagcctc gtataaatta gcgcaactct ccagccgcga aaaaaatcgc
     1321 gtgctggaaa aaatcgccga tgaactggaa gcacaaagcg aaatcatcct caacgctaac
     1381 gcccaggatg ttgctgacgc gcgtgccaat ggccttggcg aagcgatgct tgaccgtctg
     1441 gcactgacgc ccgcacggct gaaaggcatt gccgatgatg tgcgccaggt gtgtaacctc
     1501 gccgatccgg tggggcaggt aatcgatggc agcgtactgg acagcggcct gcgtcttgag
     1561 cgtcgtcgcg taccgctggg ggttattggc gtgatttatg aagcgcgccc gaacgtgacg
     1621 gttgatgtcg cttcgctgtg cctgaaaacc ggtaatgcgg tgatcctgcg cggtggcaaa
     1681 gaaacgtgtc gcactaacgc tgcaacggtg gcggtgattc aggacgccct gaaatcctgc
     1741 ggcttaccgg cgggtgccgt gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa
     1801 atgctgcgta tggataaata catcgacatg ctgatcccgc gtggtggcgc tggtttgcat
     1861 aaactgtgcc gtgaccagtc gacaatcccg gtgatcacag gtggtatagg cgtatgccat
     1921 atttacgttg atgaaagtgt agagatcgct gaagcattaa aagtgatcgt caacgcgaaa
     1981 actcagcgtc cgagcacatg taatacggtt gaaacgttgc tggtgaataa aaacatcgcc
     2041 gatagcttcc tgcccgcatt aagcaaacaa atggcggaaa gcggcgtgac attacacgca
     2101 gatgcagctg cactggcgca gttgcaggca ggccctgcga aggtggttgc tgttaaagcc
     2161 gaagagtatg acgatgagtt tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt
     2221 gacgatgcca tcgcccatat tcgtgaacac ggcacacaac actccgatgc gatcctgacc
     2281 cgcgatatgc gcaacgccca gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt
     2341 aacgcctcta cgcgttttac cgacggcggc cagtttggtc tgggtgcgga agtggcggta
     2401 agcacacaaa aactccacgc gcgtggccca atggggctgg aagcactgac cacttacaag
     2461 tggatcggca ttggtgatta caccattcgt gcgtaaataa aaccgggtga tgcaaaagta
     2521 gccatttgat tcacaaggcc attgacgcat cgcccggtta gttttaacct tgtccaccgt
     2581 gattcacgtt cgtgaacatg tcctttcagg gccgatatag ctcagttggt agagcagcgc
     2641 attcgtaatg cgaaggtcgt aggttcgact cctattatcg gcaccattca aacatctccc
     2701 caagtctact aaagtctttc aaaacccctt ataatccgtg tattaaagcc ccgttcgtct
     2761 tttgacgtct actaaagtcc ctcaaaatct acggtcagat gggggtactt atgggggtat
     2821 ttgctgttcg gtttagtgga ggtaccccca agtgaaactc aatgcccgtc aaatagacac
     2881 agccaagcca aaagagaagg cttacaagct ggccgatggt ggcggtttgt atctcttggt
     2941 aaaacctaat ggaggtaaat actggcgact taaatatcgt gtagctggta aagagaagct
     3001 attggcacta ggtgtgtatc ctgaggttac tctagccgat gctcgggcaa aacgtgaaga
     3061 tgcgaaaaga ggtatcgctg gtggtatcga tccgatggaa gcgaaacgag aggaaaagat
     3121 tgcccgggaa acgcagttaa acaacacctt caaagatatt gcccttgagt ggcacagcag
     3181 caaattaaaa aaatggtctg ctggttatgc ttcagacatc ctcgaagcct tcaacaaaga
     3241 tgtgttccct tacattggca aaaaaccaat cgccgaaatc aaaccacttg aacttctgaa
     3301 tgtgctgcgg cgcatcgagg ggcgcggtgc tacagaaaaa gccaaaaaag tgaggcagcg
     3361 gtgcggggaa gttttccgct atgcaatagt cactggtcgc gcagagtata accctgcacc
     3421 agaccttact agcgcgatgc aaggccatga atctaatcat taccctttcc ttacagctaa
     3481 agaattacct gattttttca aggcattgtc cagttactca ggaagcgcat tggttgttat
     3541 ggcggctcgt ctactgatta tcaccggctt gcggactggc gaactacgtg gcgcattatg
     3601 ggatgaaatt gatttcaaca aggctatctg ggagataccc gcttcacgta tgaaaatgcg
     3661 gcggcctcat attgtgccat tgtctgatca ggctctttcg cttattggga agattagaga
     3721 aataacaggc aattaccctc ttatgtttcc cgggcgcaat gatccaagga aaacaatgag
     3781 tgaggccagc ataaaccaag tgtttaagcg cattggctac gctggacgtg taactggtca
     3841 tgggttccgg cacactatga gcacgatttt gcatgagcag ggctataaca ccgcgtggat
     3901 agaaacgcag ctcgctcacg ttgataagaa ctcaattcgt ggcacataca accatgcgca
     3961 atatctggat ggaaggcggg agatgcttca atggtatgcc gactatatgg atgcgctgga
     4021 gaaggaggag aacgttgttc atggttcgtt tgggaaacga gcttaactgt atgaatagac
     4081 cgtgttaagc aatggtagta gacttatgta gacgaacaaa gaataggcta tgtctaggct
     4141 gatccccgaa aacccgtaca cccctacggg ctggcatagc cgtcagaatt aggggcgcgg
     4201 ggtggcgtat ggtgagagcc aaaaaacaag atttagcatg gtttgatatt agtaattatg
     4261 aatttttaaa tgatttaacc ttgccggact tgattcaaga attagagtgg cgcgattttt
     4321 tattgcgtca tgcaaaagat gacactgcta tattcaaaga agagtacgat ataaaatatg
     4381 aaaggatttt ttcgggcgat cctaacctaa ccattctcaa tgaagaagag gaagaggtcg
     4441 aagaatttat tcgcaaagta aatgatgaag ccccatcatt aagaaatgag tacgatgatc
     4501 ttccctcatt atcttcagct atgggggtta gtccagtaac attttcagag ctggccatgt
     4561 actctttctc atcgatagat caaggctttt ttaaaagaga cgaagaggat tgctacctca
     4621 aggcaaacgc tatgcttgcg agtgttactg gaaacctaag taattgttcc cctaacatca
     4681 tactcgtttc tatagattta gatgacgcca ctgatgacga aattattacc agcctgactc
     4741 accttctacc tctatggcga aaagagttaa aagtaccaga gagagagcat gtcgctcaga
     4801 aaaggattgg attaaagacc cttcagaagt tgatatctaa tagagtgata cctattgttg
     4861 atctgctgat ttggggtgag aagtcaggta aggaagtgag caatccgatg atttctgcct
     4921 tggtttttag tgatgatccg aaagatacgc aggctattaa ggaatcaata aagccttttg
     4981 cacttgaatc tataagcgag agatacaccc gcttgcttca gctttatgta aacaaagaca
     5041 gagaaatgag cttgataaaa atatctgatt taatgagtag agatttatag caagcaaaat
     5101 cctacgtaaa cgttttaagt aaatttcgcg atttccgctt tgaatacgct actgtgtatt
     5161 tcaaagcgga taatcttctc aaaaaaatat tttattttta tccgctatca cccattcacc
     5221 ggtttgttta atctcctttc tgtctactga tgtccattaa agaagatgac agaatatagg
     5281 agtaaacatg tctcaatcat ttattcgtct ttctgaggtt caacgccgta ctggttacag
     5341 caaagcgtgg atttatcggc tcatcaaaga acagcgattc cctcaatctg taaagattgg
     5401 aagccgatca attgcgttta ttgaaagtga aattgaagaa tggattaatc agcgcatcgc
     5461 tgagtcgcgt ggcgaggtag cgtaatgaat aagaaaaacc gcccattaca ggcggctaat
     5521 tcagatattc gcgtatctga tgttacgccc cttacaaaat cccttcaagc accaaagcgc
     5581 acaccgaaaa agcatcgtgc cagagtctat atgctgcgta ctggtataga gggatggaca
     5641 gaaaatgaca ttcttcgcta ctgccgtctg tcttctggtc gtaactatgc aacagagtta
     5701 gaacgccagc ttggaatcac tctggagcgt atcgacgaaa agaatcctga tggtatcgga
     5761 acacaccttc gctaccgttt ctcctgccgt ggtgatgttc tgaaagtgat cactcatatt
     5821 aaccatcttg cgaacataaa tgatcacaac ggactttctc agcaggaaat tgccgacatt
     5881 ctgaaactct acccggacgc gtttaacgcc gcttaacgga gactgaaaat gaacatcgaa
     5941 aaaagcagat taatttctga ggccgcccct catctgaacg cctctctgag cacaattaac
     6001 ggtaatgaat ttgccgcaat tgtcccggtt attcctggtc atatcggtgg gcgtgaaacc
     6061 aatattgtta gcgcaaaagc gttacacaaa gcgttgggcg tgggaaaaga cttctctaca
     6121 tggatcactg atcgcatctc tgaatatgac ttcaccattg ggcacgatta ctcagtccat
     6181 aaaactattt ccccaaattt ggggaaaagc ccgaatggcg tggcttacag aaagattaag
     6241 cagtctggca gacccggcaa agactatctg ttaagtgtcg gaatggcgaa agaactggca
     6301 atgatcgaac gcaatgatca gggtcgcgct atccgccgtt atttcatcca gtgcgaggaa
     6361 gaattacagc gtagcgttcc tgaaatcgcc gcccgctatc ttcgccagct aaaagcccgt
     6421 atcagtgccg caaacaactt taagccaatg tgcgatgcgc tgaatatggc ccgtgccgag
     6481 caggggaaaa cgacgcagca acaccactac acaaacgaga gcaatatgat ttctcgtatc
     6541 gttcttggtg ggctaactgc taagcagtgg gcgcggataa atggctattc tggcgaacct
     6601 cgcgaccata tgaacgcaga acaacttgag cacctctcat atctcgaaag caccaatatc
     6661 acgttaattg atatgggcat ggaatatgag cagcgcaaag gagaactcac ccgcctgtcg
     6721 caacgctggc tcgctaagcg tctggaggtg gtccatgttt aagccgacag gaacaccaca
     6781 acctcaaaaa cgctacaagg atgcccacgg agcactcgtt actgtcg
//
LOCUS       DAZGTR010000191         6747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGTR010000191 DAZGTR010000000
VERSION     DAZGTR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.193"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(216..1865)
                     /gene="pgi"
                     /locus_tag="ACHLCY_004573"
     CDS             complement(216..1865)
                     /gene="pgi"
                     /locus_tag="ACHLCY_004573"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP0371670.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDGKEISSHDSSTNGLINRYKAWRG"
     regulatory      2102..2298
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2390..3739
                     /gene="lysC"
                     /locus_tag="ACHLCY_004574"
     CDS             2390..3739
                     /gene="lysC"
                     /locus_tag="ACHLCY_004574"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP0371671.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDIA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3884..4810
                     /locus_tag="ACHLCY_004575"
     CDS             3884..4810
                     /locus_tag="ACHLCY_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP0371672.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4846..5637)
                     /locus_tag="ACHLCY_004576"
     CDS             complement(4846..5637)
                     /locus_tag="ACHLCY_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP0371673.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5643..>6747)
                     /locus_tag="ACHLCY_004577"
     CDS             complement(5643..>6747)
                     /locus_tag="ACHLCY_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFP0371674.1"
                     /translation="KDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLITLDGSEVPD
                     VLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYWRVEDDLTL
                     VRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDGETQALLPP
                     GLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYSDVLLAFSQ
                     LTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPFGMEIASLG
                     VKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMVNNPVALRLK
                     ELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1666 a         1755 c         1624 g         1702 t
ORIGIN      
        1 tcacagacaa ggggatttat ctgataaaaa aatgccgaca taacgtcggc attgttatta
       61 aggttattta atcaatcgtg ctatgtatta attgccgaat acgatgtaca tatcggcatc
      121 gacctgtagg cctgataaga cgcgaccgcg tcgcatcagg catcggttgc cggatgcggc
      181 gtgaacgcct tatccggcct acatatcgac gatgattaac cgcgccacgc tttatagcgg
      241 ttaatcagac cattggtcga actatcgtgg ctgctgattt ctttgccatc tttcagctct
      301 ggcagaatac ggttcgccag ctgtttaccc agctccacgc cccactggtc gaaggtgaag
      361 atgttcagaa tcacgccctg agtaaagatt ttgtgctcat acagcgcaat caacgcaccc
      421 aggctgaacg gagtgatttc acgcagcagg atggagttgg tcgggcggtt accttcgaac
      481 actttgaacg gcaccacgta gtcaagcgtt gccggatctt taccctgatc gcgatattcc
      541 tgctcaacca cttcgcggga tttaccaaac gccagcgctt cggtctgggc gaagaagtta
      601 gacagcagtt tctggtgatg atcagagagc gggttatggg tgatagccgg agcgatgaaa
      661 tcgcacggta ccattttggt tccctggtgg atcagctggt agaacgcgtg ctgaccgtta
      721 gtgcctggtt caccccagat aatcgggcca gtctggtaat ccacaacgtt accgttacgg
      781 tcaacatact taccgttgga ctccatattg ccctgctgga agtacgccgc gaaacggtgc
      841 atatactggt catacggcag aatcgcctca gtttccgcac caaagaaatt gttgtaccag
      901 atgccaatca gcgccagcag tacaggcagg tttttctcgg caggcgtggt ggagaaatgc
      961 ttgtccatcg cgtgtgcgcc agaaagcagt tcaacgaagt tatcaaagcc gatggagaga
     1021 acaatcgaca ggccaatcgc tgaccacaaa gagtaacggc cgccgaccca gtcccagaac
     1081 tcgaacatgt tggcagtatc aataccaaac tcgccaacgg ctttggcatt ggttgagagc
     1141 gccgcaaagt gtttcgcaac gtgtttttca tcacctgccg ctttcaggaa ccagtcacgc
     1201 gcgctatggg cgttggtcat ggtttcctga gtggtgaagg ttttagatgc taccaggaac
     1261 agcgtggttt ccgggtttac ttttttcagc acttcagcga tgtgagtccc atcgacgtta
     1321 gaaacaaagt gcatgttcag gtgatttttg tacggacgca gtgcttcggt caccatgtac
     1381 gggccgaggt cagaaccgcc gatcccgatg ttcactacgt cagtgattgc tttgccggta
     1441 taacccttcc actcaccgga gataatcgct tccgagaagg ttttcatctt ctccagcacc
     1501 gcgttgactt ccggcattac gtctttgcca tcaaccaaaa tcggggtatt gctacggtta
     1561 cgcagcgcta cgtgcagcac agcgcggttt tcagtgcggt tgatcttctc gccagagaac
     1621 atcgacttaa tcgcgcccgc cagatcgcac tctttcgcca gatcttgtaa tttcgccagc
     1681 gtctcttcag tgatgcggtt tttggagtaa tccaccagca tctgatcgcc gaaggttgcg
     1741 gagaacttag aaaaacgatc gccgtcttta gcaaaaagat cggcgatcgt aacgtctttc
     1801 atttcatcga agtgtttctg tagtgcctgc caggcagcgg tctgcgttgg attgatgttt
     1861 ttcattagca atactcttct gattttgaga attgtgactt tggaagattg tagcgccagt
     1921 cacagaaaaa tgtgatggtt ttagtgccgt tagcgtaatg ttgagtgtaa acccttagcg
     1981 cagtgaagca tttattagct gaactactga ccgccaggag tggatgaaaa atccgcatga
     2041 ccccgtcgtt gacaaccgcc ccgctcaccc tttatttata aatgtactac ctgcgctagc
     2101 gcaggccaga agaggcgcgt tgcccaagta acggtgttgg aggagccagt cctgtgataa
     2161 cacctgaggg ggtgcatcgc cgaggtgatt gaacggctgg ccacgttcat catcggctac
     2221 aggggctgaa tcccctgggt tgtcaccaga agcgttcgca gtcgggcgtt tcgcaagtgg
     2281 tggagcactt ctgggtgaaa atagtagcga agtatcgctc tgcgcccacc cgtcttccgc
     2341 tcttcccttg tgccaaggct gaaaatggat cccctgacac gaggtagtta tgtctgaaat
     2401 tgttgtctcc aaatttggcg gtaccagcgt agctgatttt gacgccatga accgcagcgc
     2461 tgatattgtg ctttctgatg ccaacgtgcg tttagttgtc ctctcggctt ctgctggtat
     2521 cactaatctg ctggtcgctt tagctgaagg actggaacct ggcgagcgat tcgaaaaact
     2581 cgacgctatc cgcaacatcc agtttgccat tctggaacgt ctgcgttacc cgaacgttat
     2641 ccgtgaagag attgaacgtc tgctggagaa cattactgtt ctggcagaag cggcggcgct
     2701 ggcaacgtct ccggcgctga cagatgagct ggtcagccac ggcgagctga tgtcgaccct
     2761 gctgtttgtt gagatcctgc gcgaacgcga tgttcaggca cagtggtttg atgtacgtaa
     2821 agtgatgcgt accaacgacc gatttggtcg tgcagagcca gatatagccg cgctggcgga
     2881 actggccgcg ctgcagctgc tcccacgtct caatgaaggc ttagtgatca cccagggatt
     2941 tatcggtagc gaaaataaag gtcgtacaac gacgcttggc cgtggaggca gcgattatac
     3001 ggcagccttg ctggcggagg ctttacacgc atctcgtgtt gatatctgga ccgacgtccc
     3061 gggcatctac accaccgatc cacgcgtagt ttccgcagca aaacgcattg atgaaatcgc
     3121 gtttgccgaa gcggcagaga tggcaacttt tggtgcaaaa gtactgcatc cggcaacgtt
     3181 gctacccgca gtacgcagcg atatcccggt ctttgtcggc tccagcaaag acccacgcgc
     3241 aggtggtacg ctggtgtgca ataaaactga aaatccgccg ctgttccgcg cgctggcgct
     3301 tcgtcgcaat cagactctgc tcactttgca cagcctgaat atgctgcatt ctcgcggctt
     3361 cctcgcggaa gttttcggca tcctcgcgcg gcataatatt tcggtagact taatcaccac
     3421 gtcagaagtg agcgtggcat taacccttga taccaccggt tcaacctcca ctggcgatac
     3481 gttgctgacg caatctctgc tgatggagct ttccgcactg tgccgggtgg aagtagaaga
     3541 aggtctggcg ctggtcgcgt tgattggcaa tgacctgtca aaagcctgcg gcgttggcaa
     3601 agaggtattc ggcgtactgg aaccgttcaa cattcgcatg atttgttacg gcgcatccag
     3661 ccataacctg tgcttcctgg tgcccggcga agatgccgag caggtggtgc agaagctgca
     3721 ttttaatttg tttgagtaat atttccctac tggcgtataa cgctcaccga ttgttatacg
     3781 ccatcctcac tgaaaacata gattaaataa tatctgaaaa gcaaaatcac tcctgtagaa
     3841 cagtattata atcaccgaaa aatatcattt aacaggagtg ttaatggcta ctttaatgga
     3901 aaaagatgca cttttaaacg gagccagtca gtgcatcgcg tttttatcaa atatcattga
     3961 taattgctca gtttcgtcgc atcaggattc aggcgatgct ctcaagcgat tagtcagcta
     4021 tcgcgattat ctttattcta cccccgctga acttgttgat tttacacaag gtaagattct
     4081 ccttcagcag gtaagaactc aatatcagca tgaattcaac aatactacgc attcagaaaa
     4141 taaagcatct tttgattcta tttggcagcg tctgacaaat catgaagtca caccgcaaca
     4201 gcatcccatt ggttttgttt taggtgggca acccggagca ggtaaatcat ctctgattga
     4261 attagccaaa agagagacaa aagataatat aatgattatt aacggtgatg attttcgatt
     4321 tctacaccct gattttaatt atatctacca aaattatggg gatgattttg ttacccatac
     4381 agcgaaattt tccggcgaaa cggtcgaacg tgcaattgag agagcgatag tcagtaaact
     4441 caacattgtt gtcgaaggaa catttcgtaa tgccgcgacg ccgttacaaa cattaaaaaa
     4501 actcaaagat gcaggctacc aaacagaagt catgataaaa acaacctcag cggcattaag
     4561 ctgggaaagc accaatgaac ggtatagtaa ggacaaagag gcgggaaata ttgcccgaaa
     4621 agtggataaa aaccaccatg atatcgtcac cggtttgctg gcagaaaatg cccgcaaggt
     4681 gtttgcttcg aacctgtcag ataagtttgc ggtgtattcg cgtgagaaga tgatatttag
     4741 ttcacaagcg gcaacgaatg acgatattgc aacgcttata cagaatgaaa tcagcgggaa
     4801 tacacaataa taataagagc tgatggcgtg aaataacgcc gggctttatg cccggcgcat
     4861 taccgtctgg tacattaagc tatccagttc cttaacgtcc ccttctctgc tatccggtaa
     4921 cagcggtgga atttccccac gcgccagttc cgcacgaata aacgcatgta acaatggacg
     4981 gcgcggacca tattccgctt ctcctgcgcg ctgtttacgt tccagtaatt cgtctatttc
     5041 tgcacgtaac ggcgcatcaa gttcgcttcc cgccagtaac tcagcaaagc gcattggcgg
     5101 tacgccttta cccgcttcga cccagcgcac ggccagcagc ggacgcagca cgtaaaagta
     5161 ttttttcagt cgaacttcgt cgccctgcaa atatccgggg aagtttttct gcgccatcga
     5221 atagtaatgc cagcgcgcac gcagcggaga aaaccacatt gggaccattg ccttaaacgc
     5281 cgttatcgtt tcttcatctt gttgataaac caccggagaa tccagccatt ctatcagcgt
     5341 cgggttcgcg cctttcaata atcccagcgc cttacgccac tcccaaccgg aaacatctaa
     5401 ctcgtcgtcg atgggcagct caataacatc gcgcggtgac tctactcgca gataccattc
     5461 cagcgggtga acatacaaaa accgtacatc gtagtcgctg tcgggcgagg caaatcccca
     5521 gccgcggctc cctgattcac aggcgtacag tactttcaca tcataccgcc gttcaatttc
     5581 ttttaactgc tgcgataccc tttctcgcat cgcagcactg actccattta atggcatgtt
     5641 actcaccctt tgatattaac caacccatgc agaacctgat ccagaccgcc aaatacagag
     5701 atgttatcga tccgttcagc cactctctcc agcgtttcca gttcttttaa gcgtaacgct
     5761 accgggttgt ttaccatgac tttagcggta ttgagtaacg aacgcgtcgc tgccgtttct
     5821 tcacgtcggc gaatcacatt ggcctgcgcg gatttctccg cctcaaccag ctgcgcaagg
     5881 atatttttca tatcgccagg caggacgata tctttcaccc ctaacgaggc aatctccata
     5941 ccaaatggca acatgcgcga tttcacctgt tcgctgacaa cgtcatcaat gacctgttta
     6001 tcttccagca attcatccaa cgtacgcgta cctaccgctt cacgcagggc aaattgtaat
     6061 tcccgataca aatgatcaat cggcttcgtt aactggctaa atgccaataa cacgtcgctg
     6121 tagcgccagt tagcggcgag gttaatccgc agattgacct tatctttcgt taagatttct
     6181 tgcccgctga cttccagtac ctgcaagcgg gtgtcaacca cttcagcgtc aaccagatgg
     6241 ttaattttcc agtatgcggt cagccccggc ggtaacagcg cctgcgtttc accatcgatt
     6301 ttcagcacgc ctacatgcca ggccggaacc tgcaccgtta gtattgcatc gcggccttta
     6361 actgcccctt tgcgccgtgg ttgcaatacc gcattcatca cctccgtttg tacctggact
     6421 tgctgcgtat tcatccgcac cagtgtcaaa tcatcctcta cacgccagta caaacggcgc
     6481 gttgatggcg gcaggatctc ctgcaaaata ccgtccatat acaacgcccc ggcttcaatc
     6541 tccgacaagt ctgccaccag gcaatatttc tccacccaat caggctgaaa gcggcgcaaa
     6601 taatccgcca gcacgtcggg aacttcgctg ccatcgagcg taatcagcaa gacttctggc
     6661 gtatttaacc agggtaaaag atgctcgcct gcatgtagca ctttgtagta atcgccgttt
     6721 ctggagagta atgccagctg atctttt
//
LOCUS       DAZGTR010000192         6744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGTR010000192 DAZGTR010000000
VERSION     DAZGTR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.194"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..205
                     /locus_tag="ACHLCY_004578"
     CDS             <1..205
                     /locus_tag="ACHLCY_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFP0371675.1"
                     /translation="KKICRAEGATEEDDNKLVREFERLTEHPDGSDLIYYPRDDREDS
                     PEGIVKEIKEWRAANGKSGFKQG"
     gene            267..410
                     /locus_tag="ACHLCY_004579"
     CDS             267..410
                     /locus_tag="ACHLCY_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0371676.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGL
                     ATQ"
     gene            905..1819
                     /locus_tag="ACHLCY_004580"
     CDS             905..1819
                     /locus_tag="ACHLCY_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0371677.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCYLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQATRGRKGGSKSKRGTVAT
                     SARTLKPWEKLGISRAWYYQLKKRGLVE"
     gene            complement(2044..2310)
                     /locus_tag="ACHLCY_004581"
     CDS             complement(2044..2310)
                     /locus_tag="ACHLCY_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFP0371678.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            2489..4708
                     /gene="mobQ"
                     /locus_tag="ACHLCY_004582"
     CDS             2489..4708
                     /gene="mobQ"
                     /locus_tag="ACHLCY_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFP0371679.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANVPSWCSDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     EAVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNERAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            4941..6686
                     /gene="col"
                     /locus_tag="ACHLCY_004583"
     CDS             4941..6686
                     /gene="col"
                     /locus_tag="ACHLCY_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="HFP0371680.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALSAAQERRKQKENKEKDAKDKLDKES
                     KRNKPGKATGKGKPVGDKWLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEV
                     SKDPDLSKQFKGSNKTNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNI
                     RVTTPKRHIDIHRGK"
BASE COUNT         1731 a         1556 c         1901 g         1556 t
ORIGIN      
        1 aaaaaaaata tgtagagctg aaggggctac tgaagaggat gacaataaat tagtgagaga
       61 gtttgagcga ttaactgagc acccagatgg ttcagatctg atttattatc ctcgcgatga
      121 cagggaagat agtcctgaag ggattgtcaa ggaaattaaa gaatggcgag ctgctaacgg
      181 taagtcagga tttaaacagg gctgaaatat gaatgccggt tgtttatgga tgaatggctg
      241 gcattctttc acaacaagga gtcgttatga aaaaaataac agggattatt ttattgcttc
      301 ttgcagtcat tattctgtct gcatgtcagg caaactatat ccgggatgtt cagggcggga
      361 ccgtatctcc gtcatcaaca gctgaagtga ccggattagc aacgcagtaa cccgaaatcc
      421 tctttgacaa aaacaaagcg tgtcaggctg attctgatgc gctttttttt gaaatgtcac
      481 aaaaattcca tgtgggagat gggatctaaa atccccgtgc agaactttcc atccaggggg
      541 agaaaacttg tcgttttgag ccgttcggtg ttcagaacgc acgaaaccga tcgcgcatcg
      601 ctttcgtgca tagttatgca acgcgctaaa aacgattctg acgcattttt tatgtttacc
      661 ctggtgtttc cttgtctttt tgcgtttttt gcgtcagaaa gcgattgagg gcgttttaag
      721 gggtacgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
      781 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatt cgtgtctctc
      841 cgccaagatt gtcacgagaa tacaggggcg aggtcggtcg ataacctgga aggaatcgta
      901 acacatgagc gccgcgcttc aatacttcga agaaaattta ccccaccgtc cctatcacac
      961 ggatgatctc gcttttggtc ttcgcatctc cggcaaaggg cgtgcgcttc ttgcgcggta
     1021 catccagcag aaccagcctc atgcgcagtt ctggctggtt tttgacgttg accgcgaggg
     1081 ggctgcgatt gactggagcg accggaacgc ccccgcgccg aacatcaccg taaaaaatcc
     1141 cgtgaacggt catgctcacc tgctctatgc actcaacatc gccgtgagaa ccgcgcctga
     1201 tgcgtcggtt aaggcactga agtacgctgc cgcgatagag cgtgcgctgt gtgagaaact
     1261 tggtgcagat gtgaactaca gcggactgat ttgcaaaaat ccgttccacc tggaatggct
     1321 ggtgatggag tggcgcgagg aagcctatac cctcgatgaa ctggctgatt atctcgattt
     1381 gagcgcctca gcgcgccgta gcatcgataa acattacggg atggggcgaa actgctacct
     1441 gttcgaaatg acgcgcaaat gggcttacag ggcgattcgt cagggctggc ctgtattctc
     1501 acaatggctt gatgccgtga tccagcgtgt cgaaatgtac aacgcatctc ttcccgttcc
     1561 gctttctccg gctgaatgtc gggctattgg caagagcata gcgaaataca cgcacaggaa
     1621 ctttacgccg gaaactttcg cacagtatgt ggctgatacg cacacgccag aaattcaggc
     1681 tacacgcggt cgcaagggcg gttctaagtc taagcgcggc acagtagcta catcagcacg
     1741 cacgctgaaa ccttgggaga aattagggat cagtcgcgcc tggtattacc aactgaaaaa
     1801 acgaggtctc gtagagtaga ccaaataagc ctatatcaga taacagcgcc tttttggcgc
     1861 ttttttgagc agcttggttt gttgctattt ccctcgttga atcccgcaat ggcgcggctt
     1921 tccgcatgat tgaggtggta gcgctcgccg cagtctcatg accgagcgta gcgagcgaat
     1981 gagcgaggaa gcgcaaaggt gtcggtggtg catgtggcac ttacgcgccg gggtttctcc
     2041 gttctacggt ttcgcaggtg gtttgggtag cttctccagc tcgttaatca gcggttgtat
     2101 ccggttcaca tcggcctgtc ttgtgacttc ctttcgcagg aactggagca ggaacgcacg
     2161 cagttgcgct tcttccggcc tccgtaccct cgccagcatg gcagccccca caatgacttt
     2221 ttgcgccgtg tccaggctcc ggctcttcgc cttcaggcgc tgtaatctgg cctcagcttc
     2281 ggcaatcttc tgttcgagtg ttctgctcat ttcgtgactc cgtgcgcggt gaaaaatcgc
     2341 attttagcgc gtcactggta gttgcaaaac tgaactggca taatgcacgg cacatcacga
     2401 agtgcgcact tatacaatct ccacttcgtt tcgattgtgt gcggtctgcg acgctaaaag
     2461 aaaacggcaa aaaggcatta cggcagaaat ggcgatttat catctcagca tgaaaatcat
     2521 ttcgcgaaaa aacggctaca gtgccgttgc ttctgctgcc taccgttccg gctctgtcat
     2581 acccgatgac cgtaccggat taatccacga ttacacccgt aaacgcggcg ttgatgatgc
     2641 ggtcattctc acccctgcga atgtaccgtc ctggtgtagt gaccgttccg ttctctggaa
     2701 tgcggtcgag aaagccgaac agcgccggaa ctcccagctg gcaagggaga ttgaactcgc
     2761 cattccccgt gagatttccc gcgaggccgc acgggaggcc gttctcgctt ttgtccggga
     2821 aaactttgtc agtcggggca tgattgccga tgtggcgttt catcacatgg accggaccaa
     2881 tccccatgcg cacatcatgc tgaccacgag agctgtcggg gaaacaggat ttgcaggaaa
     2941 ggtcagggac tggaacgagc gggcactcgc cgagacgtgg cgcgcatcat gggctgacca
     3001 tgcgaacaga gcgcttgcga acgccggcta ccaggaagag atagaccatc gttcatacga
     3061 gcgtcaggga ctggagaaag cgccgggcct tcacctcgga aaggctgcct gtgcgatgga
     3121 aaaacggggc atggaaacag aacgcggtga gcagaaccgc ctgattaaca gccttaacct
     3181 ggaaatacag gtttcccgca caaggctggc agtcatccag gtacaggaga acgtgcggaa
     3241 acgggaactc agcgatgccg cccgtcgtgc agcggaagcc cttaacctga ccattcccgc
     3301 tgcgaatgcc tcagcggata ccctgcggga attcattgcc acgctgccgc aggaatgcgg
     3361 gaacgcgtgg gagatgaccc cggagttcct ggcgatgagc gggaaggtga acgacatcga
     3421 acgtgagggg aatgcgctgc tgaaagagca ggccattctc gaaaaggaga tgaccggact
     3481 caaaaaagca cgccctgtcg cgtcccttct gtcagagatt cccctgatga catgggctga
     3541 accggaatac cgcaaaagac aactccgttt ctggaaactc gggaaacaga ttgaatctct
     3601 tcgccgcacc tacagggccg tgaaagaacg ggacattccc gcccgtcgtc aggcctttga
     3661 aacgcagtgg aatacgtgga ttgcgccggg aatggcagag ctgaaagaaa aactgtcagc
     3721 acgggaagcg gagcggcgca gggaggaagc cgaagcggaa gcgcgccgga aggaacagga
     3781 gcatgaggcg cggctgaaac gtcatgataa ccaccgtctg agccgtgaaa cggcattagt
     3841 cggggttatt acggagctgg ggcgtgccag agagccggga acgggcagga taacccgcta
     3901 catgatgttg agtaacagag ccggagaatt cacggtatgg ggtgatgagc tggcgcatta
     3961 cccccagagt gttcatgacc cggtgaatgt ttacctgtcg ccaggcgggg ctgtgatggt
     4021 ctcggatata cgcgagggaa tgccagaatc tcatgagacg atggcgcggc ctgagcgtgt
     4081 gagaatgtat tccggtgcga cggtccggca tgtactggaa cagatgcgcc aggggtggcc
     4141 ctcttacggt tttccggcgc tgccgcatca ctggccggat aatttttatt tcagcgacga
     4201 ccgcaggccc gtagcctctc cgctgccgtc agcgcaccgg gtggatatca ccgctgatgc
     4261 tgtaccggaa caactcatgc ctgttgtatt ttcgagagag ggaaacagca ggacgctgaa
     4321 tctgttgtta tgcaaagggc cggaggaaat gcttgtcggg tttgttcgcc gggaaggagg
     4381 gttttgcccc gttcttgtgc ttccgtcgcc ggattacagt catctgatag tcagcaccat
     4441 cacggagaac ggggtatgcc tggcaggtta cggagaagct ataaaccatg atgaggatac
     4501 tccgtaccca ccggaaccgc acctgatgca gttccggctc aaaggccatc atgacaggct
     4561 tttggctgct gtccataaac cggaagagat gccggattat ctcttccgtc aactcggttt
     4621 taatcagacc tggcatgagt ggaagcggga cgaacagcac aggcaacaac aacgccgccc
     4681 cgggcacttc cggggcatga gtatgtgata tccggggctg caccccggac cccgccaaca
     4741 catcacgggc cacaaaattt tttgtggccc gctctgcgtt ttctaagtgt tatccctcct
     4801 gatttctaaa aaattttccg cctgaacttg acagaaaaaa cgatgacgag tactttttga
     4861 tctgtacata aaaccagtgg ttttatgtac agtattaatc atgtaattaa ttgttttaac
     4921 gcttaaaaga gggaattttt atgagcggtg gcgatggacg cggccataac acgggcgcgc
     4981 atagcacaag tggtaacatt aatggtggcc cgaccgggct tggtgtaggt ggtggtgctt
     5041 ctgatggctc cggatggagt tcggaaaata acccgtgggg tggtggttcc ggtagcggca
     5101 ttcactgggg tggtggttcc ggtcatggta atggcggggg gaatggtaat tccggtggtg
     5161 gttcgggaac aggcggtaat ctgtcagcag tagctgcgcc agtggcattt ggttttccgg
     5221 cactttccac tcctggagct ggcggtctgg cggtcagtat ttcagcggga gcattatcgg
     5281 cagctattgc tgatattatg gctgccctga aaggaccgtt taaatttggt ctttgggggg
     5341 tggctttata tggtgtactg ccatcacaaa tagcgaaaga tgaccccaat atgatgtcaa
     5401 agattgtgac gtcattaccc gcagatgata ttactgaatc acctgtcagt tcattacctc
     5461 tcgataaggc aacagtaaac gtaaatgttc gtgttgttga tgatgtaaaa gacgaacgac
     5521 agaatatttc ggttgtttca ggtgttccga tgagtgttcc ggtggttgat gcaaaaccta
     5581 ccgaacgtcc aggtgttttt acggcatcaa ttccaggtgc acctgttctg aatatttcag
     5641 ttaataacag tacgccagca gtacagacat taagcccagg tgttacaaat aatactgata
     5701 aggatgttcg cccggcagga tttactcagg gtggtaatac cagggatgca gttattcgat
     5761 tcccgaagga cagcggtcat aatgccgtat atgtttcagt gagtgatgtt cttagtcctg
     5821 accaggtaaa acaacgtcag gatgaagaaa atcgccgtca gcaggaatgg gatgctacgc
     5881 atccggttga agcggctgag cgaaattatg aacgcgcgcg tgcagagctg aatcaggcaa
     5941 atgaagatgt tgccagaaat caggagcgac aggccaaagc tgttcaggtt tataattcgc
     6001 gtaaaagcga acttgatgca gcgaataaaa ctcttgctga tgcaatagct gaaataaaac
     6061 aatttgaacg atttgcacat gacccaatgg ctggcggtca cagaatgtgg caaatggccg
     6121 ggcttaaagc tcagcgggcg cagacggatg taaataataa gcaggctgca tttgatgctg
     6181 ctgcaaaaga gaaggcagat gctgatgctg cattaagtgc cgcgcaggag cgccgcaaac
     6241 agaaggaaaa taaagaaaag gacgctaagg ataaattaga taaggagagt aaacggaata
     6301 agccagggaa ggcgacaggt aaaggtaaac cagttggtga taaatggctg gatgatgcag
     6361 gtaaagattc aggagcgcca attccagatc gcattgctga taagttgcgt gataaagaat
     6421 ttaaaaactt tgacgatttc cggaagaaat tctgggaaga agtgtcaaaa gatcccgatc
     6481 ttagtaagca atttaaaggc agtaataaga cgaacattca aaagggaaaa gcaccttttg
     6541 caaggaagaa agaccaagta ggtggtaggg aacgctttga attacatcat gataaaccaa
     6601 tcagtcagga tggtggtgtc tatgatatga ataatatcag agtgaccaca cctaagcgac
     6661 atattgatat tcatcggggt aagtaaaaat ggaactgaaa catagtatta gtgattatac
     6721 cgaggctgaa tttctggagt ttgt
//
LOCUS       DAZGTR010000193         6623 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGTR010000193 DAZGTR010000000
VERSION     DAZGTR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.195"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..499
                     /gene="ykgH"
                     /locus_tag="ACHLCY_004584"
     CDS             <1..499
                     /gene="ykgH"
                     /locus_tag="ACHLCY_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP0371681.1"
                     /translation="QTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNRE
                     IKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKM
                     IYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEY
                     QQNSG"
     gene            complement(745..1440)
                     /locus_tag="ACHLCY_004585"
     CDS             complement(745..1440)
                     /locus_tag="ACHLCY_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0371682.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1433..2860)
                     /locus_tag="ACHLCY_004586"
     CDS             complement(1433..2860)
                     /locus_tag="ACHLCY_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0371683.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKNFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2871..3590)
                     /locus_tag="ACHLCY_004587"
     CDS             complement(2871..3590)
                     /locus_tag="ACHLCY_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0371684.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4117..4971)
                     /gene="rclR"
                     /locus_tag="ACHLCY_004588"
     CDS             complement(4117..4971)
                     /gene="rclR"
                     /locus_tag="ACHLCY_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP0371685.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSVRYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5197..6522
                     /gene="rclA"
                     /locus_tag="ACHLCY_004589"
     CDS             5197..6522
                     /gene="rclA"
                     /locus_tag="ACHLCY_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP0371686.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1802 a         1640 c         1443 g         1738 t
ORIGIN      
        1 ccagacaaca ccaggcatac ttgttaactg tgatagcaat aataactttg cctgtcagac
       61 cacaatgact gaagatgtta tacagcgaat tactacattt tttcacacca gcccagatgt
      121 caagaacaga gaaataaagc tggaatggtc aggagataag agagatttac caactgctga
      181 agcggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag aataccataa
      241 tggcaggcaa gttctcgatg agatacaaac accttcagca attaacagtg agctttatac
      301 aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg gctgtgtaac
      361 tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt
      421 ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata
      481 ccaacaaaac tctgggtagt aataaacagc attctgatag agagtgcctg tataaagctg
      541 aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg
      601 taaatatcct ggcattacta atgccaggat atttacagaa gagcagcata accatgacgg
      661 aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt
      721 ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca ccgctttcac
      781 cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgaac tagggccgct
      841 aatgatgtta atgcaggacg gcattcgttc accggcctgc gctttctgat gcaatttttc
      901 tgcgagttgc gctacacgcg gcaagatagt gcttttacgc aggataaaaa gagaagattc
      961 cgggagcagg ctcaatgaac gcccgcgccc ggcggcggaa aaaagaacca cgcctcccga
     1021 ttcggttaaa ccatattcag catacacgac acccacttta gcctgctcgg cctgcgagat
     1081 attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc
     1141 actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact gatctcccag
     1201 ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca gctcacagcg
     1261 cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag
     1321 ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag
     1381 tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgcctc gattatccat
     1441 tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct ccgtcagctt
     1501 caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt ggtgttttgc
     1561 cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact ttccacaatc
     1621 ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga
     1681 tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca
     1741 ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa tctttaaaat
     1801 ttttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc
     1861 catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc
     1921 gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga
     1981 caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc ggtcctgtca
     2041 gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca
     2101 atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact gcaatatgcg
     2161 ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac
     2221 ctgtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt
     2281 cgcggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc ccctcatagc
     2341 ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca
     2401 caacatgaga tggcggatct tgatccagct ggagaatgta ttcacccaga tcggtttcaa
     2461 tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg
     2521 atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atgtagcgag
     2581 tggcgtcttc tttagttttt gcaaaataga cgtgaccgcc gttttgcgtc actttttctg
     2641 agagctggta cagataagcg tcgagattac ttagaacatg atcacgtatc tgggcggccc
     2701 gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccaa
     2761 tacgctgctg cgcgtttgcc accgctttgc gcatgatcgg atcttcaatt tgctgacgga
     2821 tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc
     2881 atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg taatcgccca
     2941 ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg gacttccatc
     3001 aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac
     3061 gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc
     3121 acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc caggctacaa
     3181 gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac ccctaattta
     3241 ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc
     3301 cattcaggtt catccgccag atacgtcggg taacttttta cggcataggt gcaagagcca
     3361 gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct
     3421 ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca tccctgtttc
     3481 tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt
     3541 cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact
     3601 ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat caatcatcaa
     3661 taaatatggc gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa
     3721 tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct
     3781 cacattttat ggggaaatcg acgttcattt ttggcataag aaatccattt aaaaaggcta
     3841 catcccttat acctattcag agctacttat tcaggggtta ttgagatata cactgatggt
     3901 gtacaaaaaa atcaccgaca ataatcacgt atcaagtaat agtcggggtt ttatttaatt
     3961 gaagcaattt agtttgcatt tttatttaaa aactgagcta gagtccatca gaaaaaaata
     4021 tataaagccc aaaccaccgt tggaaataga aatagttgaa aggctcacaa ctcccggaat
     4081 gcggattatc tttgccctga tttctggcgg ttttattcag ggtgcaagct gtctgaccct
     4141 ttcccgatat tctcccggag tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga
     4201 ttcactggca tagcctactg actcagcgat caccacaaca gggagcattt cccgggaaaa
     4261 catctgggcc gctatttgta gacgcaactt tgttaataca gccagcggcg tggttccgga
     4321 aacatcacgg aaaagctggg caaaacttgc ccgggacatg tgagcgatgc tggccagcga
     4381 ttcgacggtc caggcgtgtc ctggcatttc cagcatttgc tgtattaccg caccaagacg
     4441 tggatgcaga agcaaactga gaatgttttt ctcagtatta acctgtgcaa tccactcacg
     4501 caccgcgagg gtaaagaatg tagcgcagat ctggctacac agtgcatcca ctcccggcat
     4561 cgccgatctg gattcctgtt gtaaaaacgg gatcgcctcc ctcagccagt tatattccac
     4621 gctgtggtta accggtgcta aaaacagcgt ttccggcaaa gacgttaaaa aataacgcac
     4681 cgaatgttgc aaccgaagag tgccgcagac aatacaggtc gattcattat cgacatgact
     4741 caggcgatga gctgaatttt gtggtagcag gaccacattt cccgggcgta atgtaaaaat
     4801 ctctcccgtc ggcatttcca gtttcgccgc tccttgcgtt aacgcatgcc aacgaataac
     4861 cgataattcc ccggcaccat gtggaagctg ccagtcactt cctaacacgc aattcttatc
     4921 gatcgttcct tgtggagcgt taagcatcaa caaacggcta agggcatcca tatcagactc
     4981 ctgagtggtt tttgattaca aacaccaaca atcttagtaa ataactaatc taataccatc
     5041 aaatggttaa tcatgatttt tgttttgtga ttctgattaa gaaaaagaaa ataccagacg
     5101 ataagacaaa atatcaagcg ttgtgaagaa atggtatttg ctcttttgcg tctatagtca
     5161 tgatgtcaaa tgaacgcgtt tcgacaggaa atcatcatga ataaatatca ggcagtgatt
     5221 attggttttg gcaaggctgg aaaaacatta gccgtcacgc tggcaaaagc aggttggcga
     5281 gtggctctca tcgaacaatc aaatgcaatg tatggcggga cctgtattaa tatcggctgc
     5341 atcccaacca aaacattggt tcatgacgca cagcagcaca cagattttgt ccgtgccata
     5401 cagcgtaaaa atgaagtggt taatttttta cgtaataaga attttcataa tcttgcggat
     5461 atgcccaata tcgacgtgat cgacggccag gcggagttta tcaataatca tagcctgcgt
     5521 gttcatcggc ctgaggcaaa tctggaaatt catggcgaga aaatttttat taataccggt
     5581 gcacaaaccg tggttccgcc aattcctgga attaccacca cgccaggagt atatgacagc
     5641 accggattac ttaatctaaa agaattgcct gggcatttag gtattttggg cggcggatat
     5701 attggcgttg agttcgcctc tatgttcgct aattttggca gcaaggtaac cattttagaa
     5761 gcagcttcgc tgtttttgcc tcgggaagat cgggatattg ctgataatat cgcgacgatt
     5821 ttacgcgatc agggcgtcga tattatcctc aatgcccatg tggagcgaat cagtcaccat
     5881 gaaaatcaag tgcaagtgca tagcgagcac gcccaactgg cggtggatgc actgttaata
     5941 gcttccggtc gtcaaccggc taccgcttcg ttacatccag aaaatgccgg tatcgcagta
     6001 aacgagcgcg gggcaattgt cgttgacaag caattacata ccaccgcaga caatatttgg
     6061 gcgatgggag atgttaccgg cgggctgcaa tttacttata tttcactgga tgattaccgc
     6121 attgtgcgtg atgagttgct gggagaaggc agacgtagta ctgatgatcg gaaaaatgtg
     6181 ccttattccg tattcatgac accgccactg tccagggttg gtatgacaga agaacaggcc
     6241 agagagagtg gtgctgatat tcaggtggtg acattgcctg tagctgcaat tccgcgtgcc
     6301 agagtgatga atgatactcg tggggtatta aaagcgattg ttgataataa aacccaacgt
     6361 atattagggg catcactgct gtgtgttgac tcccacgaga tgatcaatat agtgaaaatg
     6421 gtgatggatg ccgggctgcc ttatagcata ttacgcgatc agatatttac tcatccgtcg
     6481 atgagcgaat cactcaatga tctattttca ttagtcaaat aaactcaaaa tcagacgcca
     6541 gaacaaatat tctggcgtct cagagaaaag aatcttatta attccctgtt actctataac
     6601 ttcttaatca cttcattgat ggt
//
LOCUS       DAZGTR010000194         6609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGTR010000194 DAZGTR010000000
VERSION     DAZGTR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.196"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            246..371
                     /locus_tag="ACHLCY_004590"
                     /pseudo
     CDS             246..371
                     /locus_tag="ACHLCY_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            436..696
                     /locus_tag="ACHLCY_004591"
                     /pseudo
     CDS             436..696
                     /locus_tag="ACHLCY_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            746..985
                     /locus_tag="ACHLCY_004592"
                     /pseudo
     CDS             746..985
                     /locus_tag="ACHLCY_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1033..1650)
                     /gene="ureG"
                     /locus_tag="ACHLCY_004593"
     CDS             complement(1033..1650)
                     /gene="ureG"
                     /locus_tag="ACHLCY_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFP0371687.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(1662..2336)
                     /locus_tag="ACHLCY_004594"
     CDS             complement(1662..2336)
                     /locus_tag="ACHLCY_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFP0371688.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLVRLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(2337..2801)
                     /gene="ureE"
                     /locus_tag="ACHLCY_004595"
     CDS             complement(2337..2801)
                     /gene="ureE"
                     /locus_tag="ACHLCY_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFP0371689.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(2811..4514)
                     /gene="ureC"
                     /locus_tag="ACHLCY_004596"
     CDS             complement(2811..4514)
                     /gene="ureC"
                     /locus_tag="ACHLCY_004596"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFP0371690.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(4507..4827)
                     /locus_tag="ACHLCY_004597"
     CDS             complement(4507..4827)
                     /locus_tag="ACHLCY_004597"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFP0371691.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(4836..5138)
                     /locus_tag="ACHLCY_004598"
     CDS             complement(4836..5138)
                     /locus_tag="ACHLCY_004598"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFP0371692.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(5229..5927)
                     /locus_tag="ACHLCY_004599"
     CDS             complement(5229..5927)
                     /locus_tag="ACHLCY_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFP0371693.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQ"
     gene            6308..6583
                     /locus_tag="ACHLCY_004600"
     CDS             6308..6583
                     /locus_tag="ACHLCY_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371694.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         1551 a         1698 c         1735 g         1625 t
ORIGIN      
        1 ctctccttta acctcatgtg caatatccgt aacgcgctca cataaatcat catctgataa
       61 tataacaaca cccattattt tattctgacc taaaaacttc ttatgaaata aacgtttacc
      121 cgacgtatct gatcccgctg tttttacctg tatcctttaa catacttgag aagcccgctg
      181 aaaaaatata ccgtaaaggc ggtttctctg tcttccataa acctgtcgga taagaagggc
      241 attaaatgaa aaccaaaaaa gttatgatga ttgcactggt cagctctgcg ctggctcttt
      301 caggatgtgg agccatgggc acagcgatca aaaaacgaaa tctggaagta aaaacccaga
      361 tgagtgaaac ctgaggtagc ctgagtttaa cggacactcc ttcctgaaat agaatggcat
      421 cagaaggagc taataatgag cagaaaaacc caacgttact ctaaagagtt caaagccgaa
      481 gctgtcagaa cggttcttga aaatcaactt tcgatcagtg aaggcgcttc ccgattatct
      541 cttcctgaag gcactttagg acaatgggtt accgccgcca gaaaagggct cggtactcct
      601 ggttcccgca cggtggctga actggaatct gaaattctgc aactgcgtaa ggcgttaaat
      661 gaagctcgcc ttgagcgaga tatattaaaa aaagcagctg tagattcaat ctgtcaatgc
      721 aacacccctt tcaattatct ctttcactgc ccgcgacggt atcggttatc agtttgacct
      781 gctggctaac ggtgaaaccg tactgcgtga tgcatccggc gaaaaacgca ccaagggtga
      841 aaaagacggt cgtatgctca tgaatgttgg cctgaatgcg gaaattcgcg acaacatccg
      901 cttcggtctg gagtttgaaa aatcggcatc tggtaagtac aacgtggata acgcggtgaa
      961 cgccaacttc cgttattcct tctgataaaa acaaggggcc gcctgccggc cctgatacag
     1021 aggatgttgt gattacttac ccagcatgcc ctgctcttcg ataaaggcga tgatgttttg
     1081 cagaccttca ccacttttca ggttagtgaa cccccatggg cgttctccgc gcatacgtaa
     1141 agtatcacgc tccattacct cgagggaggc gcccacgtat ggtgcaaggt cagttttatt
     1201 gatcacgagg aagtcggatt tggtgatccc cggaccgcct ttacgcggga tcttttctcc
     1261 ttctgccaca tctatgacat atatggtaag gtccgccagt tcggggctga aggtcgcact
     1321 caggttgtcg ccgccgcttt ccacaaagat cagatcgaga ttaccaaatt tctcactcag
     1381 tgcttccacg gctgccagat tcattgatgc gtcttcacga atggctgtat gagggcagcc
     1441 ccccgtttcg acaccgacaa tgcgttccgc ttccagtgct ccggcctcag tgagaatgcg
     1501 ttgatcttct ctggtgtaga tgtcgttggt gactacggct aattgccagg tgtcacgcat
     1561 ggatttgcag agggcttcca gaagcgcggt tttacctgag ccgaccggac cgccgacgcc
     1621 gacacgcagc ggttgcttga ttatgttcat tttattttct cctaagaacg gaataatcgg
     1681 gaatactggg tttcatgtcg cgcggaagcg atagatgcaa gtggcgttgc tgaaccaatt
     1741 gcgtgatccg gggttgcaag ggcactgtcc atgttcgccg catagcgggc acacaggcgc
     1801 ataattaact gctgggcagc cagctggccg tagggtacca gcctgacgcc cgccatcact
     1861 gcgctttcaa tccagctgta gccaaggctc agggccaggt ctgaaacgga tatttgccag
     1921 cgtacgccga gccatgccat accggttaac tggctttgct ggcatagttt gcgccattcg
     1981 gccgggcagt cgggctgcca gtcgaccaga agtctggtga aggctgcacc gcggttacgt
     2041 tcttcatcgc gtagctcacg ggtttccctg caggccagca gataagcggt ccagcgcccg
     2101 gcactgtcag gatcgcccgc ttcacaggcc cggtatagcc ggacaagcag tggcaggtcg
     2161 acggcaaaaa aactctgttc catctgcaac tgctgccagt gttcaaaggc ggcgctatct
     2221 tctacccagc ctgcttccac tgcccactcc agtccctggg accagctgta gcctccaaca
     2281 gggaggctgt tactggcaag ctgcatcagc cgcagacgtt tttccggagt cggcattcag
     2341 tggctgtgct cctgatcgtg attatgggcg tgcgacttgc tggcgtaagc gccggcttcc
     2401 ggctcaaacg gaagatgtgc aaaatcaaca tccagcccaa actggcgcag catatcatcg
     2461 aggacgtgat cgtgatggta gcgcagctct ccgggcatta tttgcagcgg aacgtggcga
     2521 ttgccgagat gccagcaggc tttcgcgagc ataaaaggat ctgcgcagcg cactacagaa
     2581 acggcttcat cagcggcgat caccttaatg aattcatcac cgttttcgtt actgagaata
     2641 tcaccatcgc gcagcagcag accgcgtggc agcaacagtc cagcctgacg tccatcgtta
     2701 agggtgactt taatacggct ttttacccgc atgtcaacag ggagggtaac gctggcagtt
     2761 gtctgagccg gggtctccac ccgccgggtc aaataaagca tttgtgctcc ttaaaacagg
     2821 aaataacgtt gcgccattgg cagaatgtcc gccggttcac tggttatcag ttcgccgttg
     2881 atacgcactt cgtaggtttg tgaatccacg gttatatcag gcaacaggct gttgtggcgc
     2941 atatcggctt tttgtactgt gcggcagcct tttaccacag cagttgtgct gtgcaggtta
     3001 agctgttcag cgacgccatt tgctgctgct gcctgcgaca ggaaagtcac acgacagcgg
     3061 tgacgggcac tgcccaatgc agcgaacatt gggcgatagt gcaccggctg cggtgtgggg
     3121 atagagccgt tgatatcacc catcggcgcc atggctatca ttccgccttt gacgatagtc
     3181 gccggtttta cgccaaagaa cgccggggac cagagcacca gatccgccag ttttcccact
     3241 tcaatcgagc cgacttcatg agcaataccg tgggttaatg ccggattaat ggtgtattta
     3301 gcgatatagc gcttcacgcg gacgttgtcg ttatcaccac tttcttccgg taacgggccg
     3361 cgctgaactt tcatccggtg tgccacctgc caggttcgta acactacttc tccgacgcgt
     3421 cccatggcct gcgaatcgga cgaggtgagg gagaacgcgc caaggtcgtg caggacgtct
     3481 tccgcggcaa tggtttcctg gcgaatgcgc gattcggcaa aggctacgtc ctcggcgata
     3541 tccgggtcaa gatgatggca aaccatcagc atgtccagat gctcatcaat agtgttgacg
     3601 gtatagggta gcgtcggatt ggttgaggaa ggcagaatat tggggtgcgc gcaggcggtg
     3661 ataatatccg gagcatgacc accacctgca ccttctgtat ggaaggtatg gatagtgcgc
     3721 ccgccgatgg cagtcagagt atcctcaacg aatcctgatt cgttaagcgt atcactgtgt
     3781 agcgcaacct gaacgtccat ttcgtcggct acagtcagtg cgcagttgat taccgcaggt
     3841 gtagctcccc agtcttcgtg aattttgagg ccgataaccc cggccgcgac ctgctcacga
     3901 agcgcatccg gattggagca attgccttta cccagcaacc cgatattgac cggcaggctg
     3961 tctgcagcct gcagcatttg ataaatgtac catggtcctg gggtacaggt tgtggcgtta
     4021 gaacccgctg tcgggccagt accgccaccg atcatggtgg taatgccgga tgtcagcgct
     4081 tcttcagcct gctgtgggca gatccagtga atatgcgtat cgacgccacc tgcggtaacg
     4141 atcctgcctt ctgcggcaat aatttccgtg gatacgccga ttgggatagt gacgttgggt
     4201 tgtatatcag gattaccggc tttgccgata gcaaatatcc ttccatcttt gacgccgata
     4261 tcggctttaa cgatccccca gtaatcgatg atcagggcat tggtcagcac cagatcagcg
     4321 cagccggcgg agagcatttg cccctgtccc ataccgtcgc ggattacttt accgccgccg
     4381 aatttgacct cttcgccgta ggtagttaaa tcatcttcga cctcgatcca cagctcagtg
     4441 tctgccagac gaattttatc accggtggta gggccgaaca tgtcagcata ggcctggcgt
     4501 gaaatattac tcatcatttg cctccagctc acccattata tctccgcgga aaccgtgcac
     4561 gatacgggca ccgctcagcg ccaccagttc aacctcacgc ttttggcctg gttcgaagcg
     4621 cactgcagtt ccggcggcga tattgagacg atagcctctt gccttctggc gatcgaactt
     4681 cagtgccggg ttaacctccg cgaagtgata atgggaaccg acctgaattg gtcggtctcc
     4741 gtgattttca acgataatac tgcaggtagc ccggccgatg ttaagctcta tatagccagg
     4801 tttaacctta tattctccag ggatcatctg ttacctcaga ttatcggatt atggacggta
     4861 accagcttgg agccgtccgg aaaggtggct tcgacctgga tatcggggat catttctggt
     4921 atgccttcca tgacctgctc gcgactcagg acatgccgtc cttcttccat cagcgcagcg
     4981 acgcttttgc cgtcgcgagc gccctccatt ataaaagcgc taatcagggc cacggattcg
     5041 ggatagttaa gtttcaggcc gcgggccaga cgacgctctg ccagcagtgc agcggtaaac
     5101 agtaatagtt tgtctttttc tcttggagtc agttccatag cgttctctta agtcagccag
     5161 atccggggaa gtaccggtga tttgtcagtc agttgcggcc gcataaattg ccagatatca
     5221 cgcattacct actggcaacg cagattatca tcacttaaaa agcgaactgt cagtagacca
     5281 tcggtgagcg tggctccggc gtagttttcc agtggtgtga gtttttcgcg tatgtcttcc
     5341 agttgcgttt cattccccag gtagaacagc atcgttccca cccatggttt ccgggcaacg
     5401 caggtaagtt ctccattggc cacgtgcaga cgctcaatca acagcggaga accgtcgacc
     5461 catatctcca gacggttagc gagtgttccg tggctgaagg tttctccgat gaccggacgt
     5521 ccaaggcaca gaagatccca ggccagcagt gtactggagg acgacagatg aaacacggta
     5581 tgcaacgtgg cgtgagcgcc gggaaaaaaa atcgaatcct gtggcaacca ctccagcgtt
     5641 gcgttattgc acagcgtcag gttttgctga aggtgcgcct gcgcgccgct gctgcggtaa
     5701 aatttactgg caccaggcat ggtgatcagc gtatggcaat cagagtcaat tattgcgttg
     5761 atgatgagtt cgtcaccgcc gacgatacca ccgggtggat gaagcagaca gaggtggcag
     5821 gtttcttctt cgggataaaa aggacgctga acagtgagcg gaccgacatg atgacgggtg
     5881 gtgagcgtgg ttttcccgtg cagacgctga aaacggagat ccagcatggc ctgccagcct
     5941 tttttatttt gtggtaacgc ggtactgagc aatcttacgc ccctgataaa taaagataaa
     6001 acgacagtaa agagccggat ttccttgtta tataaaagat gatgcctttc cagtgagttt
     6061 tcgtctgaat aatacgaatg tttgttttcg tcatgaagcg ttccggctcc cggggctata
     6121 catcgccaga aaagcaatgc atattgtcgg gattatccag agaatcaggc agaaagatcc
     6181 gacaatgggc tgtgattatg ccatctggct cagaatgata tcaaggtgtt atggggataa
     6241 cttgctgata caatagcagt atgacagatt cattaatgcg gaaactgctc actgaaacag
     6301 agtgttcatg actgagcact ggtcgggtca tgcagatgat aatttcccct tttcttgggg
     6361 aagcgacagt attttcatgc atgcctgtct tcagtcatac ggggagagca tctgtgatac
     6421 agtccataca tttgttgcgg acgtcgctcc tgagtcactg actggtctgc aaacagttta
     6481 tctgtacacg gcagggcttc tgtgtgatat cgtttttcat atcgaggggg ggcctccaga
     6541 gatgaatgga ataataatat ttcctgatga tataatacct tagagaattt ctgtgtatgc
     6601 tattgtgaa
//
LOCUS       DAZGTR010000195         6558 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGTR010000195 DAZGTR010000000
VERSION     DAZGTR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.197"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..6236
                     /gene="efa1"
                     /locus_tag="ACHLCY_004601"
     CDS             <1..6236
                     /gene="efa1"
                     /locus_tag="ACHLCY_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFP0371695.1"
                     /translation="IHNIDKVIFKGQTLDRLYHEGYVFSDINTLSRYTLHGLGITGVH
                     TEENLLPAPSSSLINILKEHYNEDEISAKLPLAYDYILNKKESSSIPVEILNKLSELP
                     PHELLTPVLGQSVNPLGMGYSSDNGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIH
                     VRMREGTLNSQNLRQLLENSVSSCFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPT
                     IADATLSLLSVGLPGTGKLLRREQDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSA
                     PVASSLHFIAEHAKYTLLSWPEFYRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRC
                     MGLALLYLQTEDTAHYSILQENLMTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEML
                     QYQGNKYITNESLLHKTAWNQERITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTD
                     PNFGHADFLSPIDALKFIEAMIQLTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLL
                     PENDAGLSTRIQHTTTDRLANLAEPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQ
                     IPSLRLNGDVLNDYLSRTVLTPEQADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVS
                     SQTRLQRQATRVKQQLAGVLDTLQQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIR
                     DGETLHTTSVEVPEVVSRFQKLSTMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHE
                     DSTLAKINLAMDIKQLSEATLGSMIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRT
                     GGTMGKALSASARVLELPVLETVLGTWNLYNSVIQLQQATSYSETMAARVQIAFDSIS
                     LGLTAASVAFPPLIIATGPIAAIGMGASSIARNVARKEERHTQWLEYKKFLTDGSKHI
                     VVASPERGLLDFSGNKVFGKMVLDLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREK
                     VGYANSISPYSSLAHGYANSKWPRTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWI
                     VWREAVPDSTSRHKRPPLEVLNSQCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKD
                     YKFILRGGSGGLAVQVGGAGYYDIDANPVAKENTLSFRGLPEEFPLTFDLSKQTQSVM
                     LKTPDDEVPVMTITQKGINTLVGTAAGKDRLIGNDKDNTFHTSSGGGTVISGGGNNRY
                     IIPRDLKTPLTLTLSSNSVSHEIFLPETTLAELKPVAFELSLIYWAGNNINVQPEDEA
                     KLNHFAGNFRVHTRDGMTLEAVSRENGIQLAISLCDVQRWQAVYPEENNRPDAILDRL
                     HDMGWSLTPEVRFQGGETQVSYDPLTRQLVYQLQARYSEFQLAGSRHHTTAVTGTPGS
                     RYIIMKPVTTQILPTQIILAGDNDHPETIDLLEASPVLVEGKKDKNSVILTIATIQYS
                     LQLTISGIEESLPETTRVAIQPQDTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLEN
                     LMALNQVMTFLPRVSGSAEQVLCLENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVN
                     ISDLSIPPYSRLYLIFEGKNNVLLRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKP
                     DNEAPSLILSEFRRFTISSDKTFSLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLR
                     LTFRDFFTEVMDTTDREILEKELRPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIV
                     AEYARIQKEETSKILYQYQGAMKKKTDGPSVVEDAIMTTTVTTDSGELFPTFHPWYTD
                     DLSGRYKSVPMARKADTLYHLTPKGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNL
                     SILSEIPQNNNTVVHSILRVNGPTMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSY
                     DSHSSGRVYSREEYMMWELQQRVSEASSARTQDYWLMDAAVRNGEWKITPELLRHTPG
                     YIRSTVSKWSRGWLKTGTILQTPEDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAG
                     ADIADNAPGETRCTLRPGTCFEVTSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDN
                     LFN"
     gene            6408..>6558
                     /locus_tag="ACHLCY_004602"
     CDS             6408..>6558
                     /locus_tag="ACHLCY_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFP0371696.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKISVEAALNAEMS
                     HHLGYD"
BASE COUNT         1966 a         1464 c         1478 g         1650 t
ORIGIN      
        1 ccattcataa tatagataaa gttatattta aagggcaaac actggatcgt ctttatcatg
       61 aaggatatgt attttctgat atcaatacct tgtctcgtta tacactacac ggactaggaa
      121 taactggtgt acatactgaa gaaaacctgc taccagctcc ttcatcgtcc ttaattaata
      181 tattgaaaga acattataat gaagatgaaa ttagtgcgaa attaccacta gcatatgatt
      241 acattttaaa taaaaaagaa tcaagctcta ttcctgttga aattttgaac aaactttcag
      301 agttaccacc acatgaacta ctcacacctg ttcttggcca gagtgttaat cctctgggca
      361 tgggctactc atccgataat ggaaaaatca cagagcaagt aatagtcagt ggagctgatg
      421 gatttgataa tcccatatct ggacttatat atacctatct tgaagatcta tataacatcc
      481 atgtaaggat gcgagaaggt acactaaatt cacagaatct tcgtcagctt ctggaaaact
      541 ctgtttcttc atgctttttg actgagcaaa gtattaataa attacttagt gaggcagaaa
      601 aaaggcctta tcagtcttta acagaaatac atcagcatct cacaggatta ccaactattg
      661 ccgatgcaac cctttcatta ctttctgttg gattacctgg tacgggaaaa ctattgcgca
      721 gggagcagga ctatgggcgt ccaccagtta cagcaattca ggattccaca tttgtactcc
      781 cttataattt caaaggtatt ggttttaacg ataacattat atcctctgca cctgtagcct
      841 cctcattaca ttttatcgct gaacatgcga aatatacttt attgtcatgg cctgagtttt
      901 atcgtcatca tgcacagcga tggttcgaaa tggctaaagg atatggaagc cagaatattg
      961 attttcaccc tcagtctcta ttggtaaccc aagaaggacg ctgtatggga ttagccttac
     1021 tttatttaca gactgaagat actgctcatt atagcattct ccaggaaaac ctaatgactg
     1081 tgagtgcact tcatcagacc agtaatcgcg ataagttgcc actgtccaaa gatgataatt
     1141 ccttaatgac aagaacttat agtctgattg aaatgctaca gtatcaggga aacaaatata
     1201 ttaccaacga atcgctacta cataagaccg catggaacca agaaagaata actttattat
     1261 tcaatgaaaa aggagttaag cgagccctga taagcacgcc taatcatact ctggttctgc
     1321 aacaactgga ggatatttac cggctcactg atccaaattt tgggcatgca gatttccttt
     1381 cacctataga tgctctgaag tttattgagg ctatgataca attaactcca acacttcagg
     1441 aatattatgg cctattaaac aaagacatta ataaacatat acaagtacat tatgccgaat
     1501 cagatatggt ctggaataag cttctgccag aaaatgatgc tggactgagc accagaattc
     1561 agcacaccac caccgaccgt ctggcgaatc tggctgaacc agtcgctgtt gcaggtatct
     1621 ccctgccagt aaaaacactt tatgatatcg gagccaccct tgacggtcgg cgcatcacct
     1681 ctcctccaac atcggagcaa atcccttctc tgcgtctcaa cggtgatgtt ctgaatgatt
     1741 atctgtcccg cacagttctg actccagaac aggctgataa cataagaaaa atactgcaca
     1801 ctcagggaat acgcagcggt acccgtccca tagatccgga gatgattcgt gggacgcagg
     1861 atgacctagt ttcgtcacag actcgtctgc aaaggcaagc aacacgggtt aaacagcaac
     1921 tcgccggtgt acttgatact ctgcaacagc acttccagaa cattccacgt tcatccggtc
     1981 gtcatctttc tgtagagaat attgagctgg ctgatatcgg aagtgggcgt ttcaaccttc
     2041 aaattcgaga tggagaaaca ttgcatacca cttctgtgga agtaccggaa gttgtgtccc
     2101 gttttcagaa actttctacc atgctttcag ccctgcctgc cagtggaatc atggatttcg
     2161 acctcggcat gagtgtggtc ggagtcgtcc agtatgcccg cctgctacag caggggcacg
     2221 aagacagtac cctagccaag ataaatctag ctatggatat caagcaactt tccgaagcaa
     2281 ctctcggcag catgattcag attgccggga ataagtttct caatacagaa ggaatccagg
     2341 ggttcagact ggaaagcgcc gtcgctgaag gtatgcgctc agtagcaacc cgtaccggag
     2401 gcacaatggg gaaagccctt tctgccagtg cccgtgttct tgaactgcct gtactggaaa
     2461 cagttctggg gacatggaac ctgtacaaca gcgtcattca gctccagcaa gctacatctt
     2521 attctgagac aatggctgcc cgggtacaga ttgcgtttga ttccatttct ctgggattaa
     2581 ctgccgcttc ggtagctttc ccgccactaa ttattgccac tggccccatt gcggctattg
     2641 gtatgggagc ttccagtatt gcacgtaatg tggcacggaa agaagaacgg catacacaat
     2701 ggctggaata taaaaaattc ctgactgatg gcagtaaaca cattgttgtg gcctctccgg
     2761 aaagaggtct gctggatttc tccggaaaca aagtttttgg aaaaatggtg ctggatctgc
     2821 gtcagtctcc tcctctcttg catggagaaa gctcttttaa cgctgaccgc aaaatcggtc
     2881 atcgtccgga tctgggggac tggcaaattc gtgagaaggt ggggtatgcc aacagtatca
     2941 gtccctactc ttctctggcg cacggttacg ccaacagtaa atggccacga acaataccga
     3001 aaattccctc gggagaatat gacaccataa ttctgggcta cggtcaccag tatcaggcca
     3061 atacggaaat agaatatctg tctaactgga ttgtatggcg ggaagccgta ccagacagta
     3121 cttcccgcca caaacgtcct cctctggagg ttcttaatag tcagtgtact gtgatagctg
     3181 gagagcgtaa aaccacagta cttcccctga gagtgctcag cgatctgaca ccggaatgca
     3241 cagaacaggc tatatcgtta aaagattata aattcatact gagaggggga agcggtgggc
     3301 tggctgttca ggtcggtggc gcgggatatt atgatattga tgcaaatcct gtggcaaaag
     3361 aaaatacgct ctcttttcgc gggctaccgg aagagtttcc gctcaccttt gatttatcaa
     3421 aacaaacaca gtcggtcatg ctgaaaacgc cagacgatga ggtgccggta atgaccatta
     3481 cccagaaggg aataaacacc ctggtaggta cagccgctgg taaagaccga ctaatcggta
     3541 acgataagga caataccttc catacaagct ctggcggcgg tacagtcatc tccggaggcg
     3601 ggaataaccg ctatattatc ccccgggatt taaaaacgcc gttgacactg acgctgtcca
     3661 gtaactcagt ctctcacgaa atctttctgc cagaaacaac cctagctgaa ttaaaacctg
     3721 tcgcctttga gctgagtttg atttactggg ccgggaacaa cataaatgtt caaccagagg
     3781 atgaagcaaa actgaaccac tttgccggaa acttcagggt gcatacccgt gatggcatga
     3841 ctctggaggc ggtttcccgg gaaaatggta ttcaactggc gatttcatta tgtgatgttc
     3901 aacgctggca ggctgtttat ccggaagaaa ataacagacc ggatgccata ctggacaggc
     3961 tgcatgatat gggctggagt ctgacaccag aagtccggtt ccaaggagga gaaacacaag
     4021 tcagctatga tcccctgact cgtcagctcg tttaccagct tcaggcgcgt tactctgaat
     4081 tccagttggc cggtagtcgc caccatacca cggctgtaac cggaactccg ggaagccgat
     4141 acattatcat gaagccagtt acaacacaga tattaccgac acaaatcata ctggctggtg
     4201 ataatgacca tccggaaacg attgatttac tggaagctag tcctgttctg gttgaaggga
     4261 aaaaagacaa aaacagcgtg atattaacga ttgctacgat tcagtattcc cttcaactga
     4321 caatatccgg gatcgaagaa tcgctgcccg agacaacccg tgtggcaatt cagcctcagg
     4381 atacccgttt actgggtgac gtactccgga tcttaccaga taatggtaac tgggtgggga
     4441 ttttccggag tggtcataca ccaacggtaa accggctgga aaatttgatg gcactgaatc
     4501 aggtaatgac gttcctgccc cgggtatccg gaagtgcaga gcaggtatta tgcctcgaaa
     4561 acctaggtgg cgtaaggaaa aaagtggagg gggagttact gtcagggaag ctgaaaggtg
     4621 cgtggaaagc cgaaggtgaa cctactgttc cggtaaatat ctcagatcta agtatcccac
     4681 cctattcacg tctgtatctg atttttgaag ggaaaaataa tgtgttgcta cgcagtaaag
     4741 tacatgcagc tccgttgaaa ataacatccg caggagagat gcagttatct gaaaggcagt
     4801 ggcaacagca ggaacatatt attgtcaagc ccgacaacga agccccctca ttaatactca
     4861 gtgaatttcg tcgtttcact atttcatcgg ataaaacatt ttctttaaaa ctgatgtgcc
     4921 atcagggtat ggtccgcatc gaccgcagat cattatcggt cagattgttc tatctgcgtg
     4981 aacagccagg tatcggcagt ttacgtctga cgttcagaga ttttttcaca gaagtgatgg
     5041 atacaacgga cagggaaatt ctggagaaag agctgagacc aattctgata ggagatacac
     5101 accgctttat caacgctgca tacaaaaatc atctgaatat ccagttagga gatggcgttc
     5161 tgaatctggc agacattgtt gcggaatacg cccgtattca aaaggaagaa acatcaaaaa
     5221 tattgtatca atatcaaggt gccatgaaaa aaaaaacaga tggaccatct gtggtagaag
     5281 atgccattat gaccactact gtcacaacag attcaggtga actattccct accttccacc
     5341 cgtggtatac agatgattta tcagggcgtt ataagagcgt acctatggca agaaaagcag
     5401 atactttgta tcacctgaca ccaaaaggtg atctacagat aatatatcag gtagctacaa
     5461 aaatggtgaa tcaggcgatg attgtatccc tgccaaacta ccgacacgag tgggaaaaat
     5521 ataatttaag catcttatcc gaaatccctc agaacaataa tactgttgta cattcaatcc
     5581 tcagggttaa tggccccaca atgcaggtgc gcacaattga ctacagagga acggatgaaa
     5641 acaatcccat agtatctttt tcagatacaa ccttcatcaa tggtgaacag atgttgagtt
     5701 atgactcgca ttcatcaggg cgagtctatt ccagagaaga atatatgatg tgggaattgc
     5761 agcaacgggt atcagaagct tccagtgccc ggacacagga ttactggctg atggatgcag
     5821 cggtaagaaa cggagaatgg aagatcacac cagaattatt acgtcacaca ccgggatata
     5881 tccggagtac ggtatcgaaa tggtccagag gatggctgaa aaccggcaca atactccaga
     5941 ctccagaaga cagaaatacg gatgtatacc tgactaccat acagaacaat gtatttagtc
     6001 gtcagggggg cggctaccaa gtgtattatc ggattgatgg aatggctggt gcggatatag
     6061 cggataatgc accaggggaa acccgctgca ccctcaggcc cggaacatgt tttgaagtga
     6121 caagtgtgga tgaaaggcat tatgagtgga atatcattta tgtcacgctg aaaacctgtg
     6181 gctggagccg aaatggccaa agcaaaacgc cgaatggtga caaccttttt aactaattaa
     6241 aatctctgtt aaagatgtat aacccggcat ctatgtgctc accagcatat agatgcaatg
     6301 tgattcccct gatggtcgta tcagagatgg tatgcgagcc tgtacataga tttgtgtaat
     6361 tgcctgattt tgatatgttc aatccaacat caaaagcagg ttaatttatg gacgaaaaac
     6421 agttgcaggc tctggctaac gaactggcca aaaatctcaa aacccctgaa gatctcagcc
     6481 agttcgatcg cctgctgaag aaaatcagcg ttgaggcagc tctcaacgct gaaatgtccc
     6541 accatctggg ctacgata
//
LOCUS       DAZGTR010000196         6555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGTR010000196 DAZGTR010000000
VERSION     DAZGTR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.198"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1090
                     /locus_tag="ACHLCY_004603"
     CDS             <1..1090
                     /locus_tag="ACHLCY_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0371697.1"
                     /translation="FADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLG
                     IMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQPYLV
                     INKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNCNGNY
                     MQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPITVSSD
                     EMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLA
                     CSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEI
                     TTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            1280..1393
                     /gene="yhiY"
                     /locus_tag="ACHLCY_004604"
     CDS             1280..1393
                     /gene="yhiY"
                     /locus_tag="ACHLCY_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP0371698.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1456..2523
                     /gene="yhiI"
                     /locus_tag="ACHLCY_004605"
     CDS             1456..2523
                     /gene="yhiI"
                     /locus_tag="ACHLCY_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0371699.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2520..5255
                     /gene="rbbA"
                     /locus_tag="ACHLCY_004606"
     CDS             2520..5255
                     /gene="rbbA"
                     /locus_tag="ACHLCY_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP0371700.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFSVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5255..6379
                     /gene="yhhJ"
                     /locus_tag="ACHLCY_004607"
     CDS             5255..6379
                     /gene="yhhJ"
                     /locus_tag="ACHLCY_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0371701.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         1525 a         1634 c         1832 g         1564 t
ORIGIN      
        1 gttcgctgac agaacctatt tctccaattg ttcacctgtt gatcccacaa gtaccagacc
       61 aacctatttt ggcgataccg atggctcagt cggtgcagtt ttgtacgcgc tttttgcaac
      121 gggacatttg ggaatcatgg ctgaaggaga aaatttcctt agccagttgc ttaatataga
      181 agatgaagtt ctcaacgtat tgctaaggga gaactttaat gagcaacttg acactaatgt
      241 taatataata atcagtatcc tgaacagaag agacattgtt ttagaaagtc ttcagcctta
      301 tttggttatt aataaagacg cagtcacgcc atgtacgttt ctgggcgacc agactggcga
      361 tcgatttagt aatatctgtg gcgataaatt tatcatcgat ttattaaaac gcataatgag
      421 tatcaatgaa aacgttcatg tattagctgg aaatcatgaa acgaattgca atggtaacta
      481 tatgcagaac ttcacgcgca tgaaaccact tgatgaggat acctacgctg ggataaaaga
      541 ttatcctgta tgtttctatg gccccaaata caaaataatg gcaaatcatc atgggataac
      601 ttttgatgat cagcgaaaac gttacattat aggaccgata acagtctcta gtgatgaaat
      661 gaccaatgct ctcgatccgg tcgaattagc ggcaattatc aataaaaagc accatgctgt
      721 aattaatggc aaaaagttta aaaatagccg ggctatctct tgtcgatcct ttaatcgtta
      781 tttttctgtt tcgacagatt acagacctaa acttgaagca ctattagcat gctcacaaat
      841 gttaggtata aatcaagttg ttgcacataa cggtaatggt ggaagagaat gtataggtga
      901 aacggggaca gttttaggtc ttaacgcccg tgacagtaaa catgctggaa gaatgttcag
      961 catgcataat tgccaaatta accctggtgc agggcctgag ataaccactc cctggaagtc
     1021 ttaccagcat gaaaaaaaca gaaatggact tatgcctttg attagaaggc gcacaatgtt
     1081 acagctctga accatagaca acatgaatat taatgttact atctggttga gactgttgat
     1141 aaaacgtaaa aaggaatgct ctgttcctgc aactgaacag gcgtgaaacg cgatccaaca
     1201 ggatggatac cgttttgtca gccagtttta tagatcacac ttatgagtat gcgtgtttca
     1261 tttacggcca ggaatgctga tgatgacaac attactaccc gttttcacta agccttcccc
     1321 gttggcgctc aatgctctgc gcgctggtcg tatttgtcgt ttccttctta tcccggatgg
     1381 aagaatccgc tgaacgtcgt tcggcggcag ttgtaacacg cgtcgtttgc ttacagggat
     1441 tatggcgggc cgattatgga taagagtaag cgccatctgg cgtggtgggt tgtcggggca
     1501 ctggcggtgg ctgccgtcgt ggcgtggtgg ctgttgcgcc cggcgggtgt gccggaaggc
     1561 tttgctgtca gcaatgggcg cattgaagcg acggaagtgg atatcgccag caaaattgcc
     1621 gggcgtatcg acaccattct ggtgaaagaa ggccagtttg ttcgcgaagg tgaagtgctg
     1681 gcgaagatgg atactcgcgt gttgcaggaa cagcgactgg aagccatcgc gcaaatcaaa
     1741 gaggcacaaa gcgccgttgc cgccgcgcag gctttgctgg agcaacgaca aagcgaaact
     1801 cgcgccgcgc agtcgctggt taatcaacgc caggcagaac tggactccgt agcaaaacgt
     1861 catatgcgtt cccgttcact ggcccaacga ggggctattt ctgcgcaaca gctggatgac
     1921 gaccgcgccg ccgctgagag cgcccgagct gcgctggaat cggcaaaagc tcaggtttct
     1981 gcttctaaag ccgctataga ggcggcacgt accaatatca ttcaggcgca aacccgcgtc
     2041 gaagcggcac aagccactga acggcgcatt gccgcagata tcgatgacag cgaactgaaa
     2101 gccccgcgtg acggacgcgt gcagtatcgg gttgccgagc caggggaagt gctggcggca
     2161 ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct atatgacctt cttcctgcca
     2221 accgaacagg cgggcacgct gaaactgggt ggtgaagccc ggctgatcct cgatgccgcg
     2281 ccagatctgc gtattccggc gaccatcagt tttgtcgcca gtgtcgccca gttcacgcca
     2341 aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt tccgcgtcaa agcgcgtatc
     2401 ccaccggaat tactccagca gcatctggaa tatgtcaaaa ccggtttacc gggcgtagcg
     2461 tgggtgcggg tgaatgaaga acttccgtgg cctgacgacc tcgtggtgag gttgccgcaa
     2521 tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca actggcgggc gtgagccagc
     2581 attatggaaa aaccgttgcg ctgaacaata ttactctcga tattccggcc cgctgtatgg
     2641 tcgggctgat tggtccggat ggcgtcggga agtcgagctt attgtcgttg atttccggtg
     2701 cccgcgtcat tgagcagggc aatgtgatgg tgctgggcgg cgatatgcgc gacccgaagc
     2761 atcgccgcga cgtctgcccg cgcatcgcct ggatgccgca ggggctgggc aaaaatctct
     2821 accacacctt gtcggtgtat gaaaacgtcg attttttcgc tcgcctgttc ggtcacgaca
     2881 aagcggagcg ggaagtgcga attaatgagc tgctgaccag caccgggtta gcaccgtttc
     2941 gcgatcgtcc ggcagggaaa ctctccggcg ggatgaagca aaaactgggg ctgtgctgtg
     3001 cgttaatcca cgacccggaa ctgttgattc ttgatgagcc aacaacgggg gttgacccgc
     3061 tctcccgcgc ccagttctgg gatctgatcg acagtattcg ccagcggcag agcaatatga
     3121 gcgtgctggt tgccaccgcc tatatggaag aggccgaacg cttcgactgg ttggtggcga
     3181 tgaatgccgg agaagtgctg gcaaccggca gcgccgaaga gttgcggcag caaacgcaaa
     3241 gcgccacgct ggaagaagca tttataaatc tgttaccgca agcacaacgc caggcgcatc
     3301 aggcggtagt gatcccaccg tatcaacctg aaaacgcaga gattgccatc gaagcgcgcg
     3361 atctgaccat gcgttttggt tccttcgttg ccgttgatca cgtcaatttc cgcattccac
     3421 gcggggagat ttttggattc cttggttcga acggctgcgg taaatccacc accatgaaaa
     3481 tgcttaccgg actgctgccc gccagcgaag gtgaggcgtg gctgttcggg caaccggttg
     3541 atccaaaaga tatcgatacc cgccgtcggg taggctatat gtcgcaggcg ttttcgctct
     3601 ataacgaact caccgtgcgg caaaaccttg agttacatgc ccgtttgttt cacatcccgg
     3661 aagcggaaat tcccgcgcga gtggctgaaa tgagcgagcg ttttaagctc aacgacgttg
     3721 aagatgttct gccggagtca ttgccgctcg gcattcgcca gcggctttcg ctggcggtgg
     3781 cggtgattca tcgcccggag atgttaatcc tcgatgaacc tacttctggc gtcgatccgg
     3841 tggcgaggga tatgttctgg cagttgatgg tcgatctctc gcgccaggac aaagtgacta
     3901 tcttcatctc cacccacttt atgaacgaag cggaacgttg cgaccgcatc tcactgatgc
     3961 acgccggaaa agtgcttgcc agcggtacac cgcaggaact ggttgagaaa cgcggagccg
     4021 ccagtctgga agaggcattt atcgcctatt tgcaggaagc ggcagggcag agcaacgaag
     4081 ccgaagcgcc gcccgtggta cacgacatca cccacgcgcc gcgtcaggga tttagcctgc
     4141 gccgtctgtt tagctacagc cgccgcgaag cactggaact gcgccgcgat ccggtccgtt
     4201 cgacgctggc gctgatggga acggtgatcc tgatgctgat aatgggttac ggcatcagta
     4261 tggatgtgga aaacctgcgc ttttcggtgc tcgaccgcga ccagaccgtc agtagccagg
     4321 cgtggacgct caatctctcc ggttcccgtt actttatcga acagccgccg ctcaccagtt
     4381 atgacgagct tgatcgtcgg atgcgtgcgg gcgatatcac ggtggcaatt gagatccctc
     4441 ccaatttcgg gcgcgatatc gcgcgtggta cgcctgtgga actcggcgtc tggattgacg
     4501 gagcgatgcc cagtcgtgcc gaaacggtaa aaggttacgt gcaggcaatg caccagagct
     4561 ggttacagga tgtggcgagc cgacaatcca cacccgccag ccaaagcggg ctgatgaaca
     4621 ttgagacgcg ttatcgctat aacccggacg taaaaagcct gccagcgatt gttccggcgg
     4681 tgatcccgct tctgctgatg atgatcccgt caatgctaag cgcccttagc gtggtgcggg
     4741 aaaaagagct tgggtcgatt atcaaccttt acgtgacccc caccacgcgt agtgaatttt
     4801 tgcttggtaa acagttgcca tacatcgcgc tggggatgct gaactttttc ctgctctgcg
     4861 gcctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag tttcctgacg ctcaccctgg
     4921 cggcgctgct gtatatcatc attgccaccg gaatggggct gctgatctcc acctttatga
     4981 aaagccagat tgccgccatt ttcggaacgg cgattatcac gttgatcccg gcgacacagt
     5041 tttccgggat gatcgatccg gtagcttcgc tggaagggcc tggacgttgg atcggcgagg
     5101 tttacccgac cagtcatttt ctgactatcg cccgcgggac gttctcgaaa gcgctggatc
     5161 tgactgattt gtggcaactt tttatcccgt tgctgatagc catcccgctg gtgatgggct
     5221 taagtatcct gctgctgaaa aaacaggagg gatgatgcgc catttacgca atatttttaa
     5281 tctgggtatc aaagagttgc gcagtctgct cggtgataaa gcgatgctga cgctgattgt
     5341 cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt acgccaggat cgttgaacct
     5401 cgcgccgatc gccattgccg atatggatca atcgcagtta tcgaaccgga tcgttaacag
     5461 cttctatcgt ccgtggtttt tgccaccgga gatgatcacc gccgatgaga tggatgccgg
     5521 actggacgcc ggacgctata ccttcgcgat aaatattccg cctaattttc agcgtgatgt
     5581 cctcgccgga cgccagccag atattcaggt gaacgtcgat gccacgcgca tgagccaggc
     5641 atttaccggc aatgggtata tccagaatat tatcaacggt gaagtgaaca gctttgtcgc
     5701 gcgctaccgt gataacagcg aaccgttggt atcgctggaa acccggatgc gctttaaccc
     5761 gaaccttgat cccgcgtggt ttggcggggt gatggcaatc atcaacaaca ttaccatgct
     5821 ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt gaacacggca cggtggaaca
     5881 cttgttggtg atgccgataa cgccgtttga gatcatgatg gcaaaggtct ggtcgatggg
     5941 gctggtggtg ctggtggtgt cgggattatc actggtgctg atggtgaaag gcgtgctggg
     6001 tgtaccgatt gaaggctcga tcccgctgtt tatgctgggc gtggcgctca gtctgtttgc
     6061 caccacgtca atcggcattt ttatggggac gatagcgcgt tcaatgccgc aactggggct
     6121 gctggtgatt ctggtgctgc tgccgctgca aatgctttcc ggtggatcca cgccgcgcga
     6181 aagtatgccg cagatggtgc aggacattat gctgaccatg ccgacgacac actttgttag
     6241 cctcgcgcag gccatcctct accggggtgc cggattcgaa atcgtctggc cgcagttcct
     6301 gacgctgatg gcaattggcg gcgcgttttt caccattgca ctgctgcgat tcaggaagac
     6361 gattgggaca atggcgtaaa tacaattgcc cgatgcgtaa gcttataggg actacgcatc
     6421 ggtgcaattt attgaatttt agttgttttt aggccggata aggcgtttat gaactgaccc
     6481 ccaaaacctg gacagtttca gttaaccggc tttttgatac tcagtcctgt attcagggca
     6541 agattacgaa agccc
//
LOCUS       DAZGTR010000197         6493 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGTR010000197 DAZGTR010000000
VERSION     DAZGTR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.199"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(25..213)
                     /locus_tag="ACHLCY_004608"
                     /pseudo
     CDS             complement(25..213)
                     /locus_tag="ACHLCY_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558227.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(266..1264)
                     /gene="lpxM"
                     /locus_tag="ACHLCY_004609"
                     /gene_synonym="msbB"
     CDS             complement(266..1264)
                     /gene="lpxM"
                     /locus_tag="ACHLCY_004609"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0371702.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            complement(1338..3059)
                     /gene="cptA"
                     /locus_tag="ACHLCY_004610"
     CDS             complement(1338..3059)
                     /gene="cptA"
                     /locus_tag="ACHLCY_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0371703.1"
                     /translation="MHLNTGQNRPTFSWCALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            complement(3153..4259)
                     /locus_tag="ACHLCY_004611"
     CDS             complement(3153..4259)
                     /locus_tag="ACHLCY_004611"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0371704.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            complement(4259..5080)
                     /locus_tag="ACHLCY_004612"
     CDS             complement(4259..5080)
                     /locus_tag="ACHLCY_004612"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0371705.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            complement(5681..5815)
                     /locus_tag="ACHLCY_004613"
     CDS             complement(5681..5815)
                     /locus_tag="ACHLCY_004613"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371706.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT         1815 a         1593 c         1391 g         1694 t
ORIGIN      
        1 accgctcagt aaatatcaat acagttctga cgagccgctt acaagctgtg attcatacag
       61 actgagcaca cgggctgcga cagcaacacc gatgcgttca gcaagcttca gggcttcact
      121 gcgaaattca ggcgaatgct atttacgggg ttttttactg gttggtactg cttttttgtc
      181 atatgagcca cctctgactg agagtttact cacttagcgt gtgtccacta ttgctgggta
      241 agctcattat cctgaaaaac ctgcctcagt aaggatcctc ttccccttct ttacgcgttt
      301 tcagcaactt gaggatccac gtatactgct caggatgtgg acgaacgaaa ttttcaacca
      361 cttcgtttat ctgtcgcgca atatgagcat cactcttctg tcgtatagca agtggcgggc
      421 tgacatgaat agtcagatga tgggtctttc cgtcataaac agggaaaagc ggtattatcc
      481 gtgcaccact gatcctgctg agccgtccga taaccggtaa agttgcttta tacgtcgcaa
      541 aaaaatctgc aaactcactg aattccgggc catgatcctg atcaggaaga taatatcccc
      601 agtatccctg ccggactgag cggacaaagg aggcaatacc gtcattacgt gcgtgcagtt
      661 ttcctccgaa acgacgacga acactgttcc agacataatc caccaccgga tttctctggt
      721 tatgaaacat cgctgccatt tttctgcccg acgcagccat cagcattgcg ggaatatcca
      781 ctccccaggc atgtggcaca agaaaaatga ccttttcatt gttctgtgcc atcttctcca
      841 caatctccag accattccag cgtatacgat gcgaaatttt atcaggcccg gacagtgcta
      901 attcagccat aagcacaacg gccatggagg ctgttgcaaa catggcatca acgatattct
      961 ccttttcttt atcactgtac tccggaaaac aaagcgataa attaatcaag gcccgttgac
     1021 gtgcgctttt ccccagacgc ccaaccagca tgcccaattt tcccaacaat ggatctctca
     1081 gtgcgggggg gaccattgca aacacacaga taataccgat acctgtccac gtcatccagt
     1141 aacgggggaa gcataatgta aggttaaagc ggggaataaa ctcagttctg atatttttca
     1201 tatttaccgg tatctgcctg cttatctcat tatgataaca gagtacagca gaaggtggta
     1261 ccatccctgg aggccaccgg acagcagagg aaaacacatc ctcctgcctt cagaacggaa
     1321 ccgaagataa gaaaatacta tggctcacca tacggaagag tgtcgaaatc agtgagccca
     1381 ttctttttat acggatttcc aatccagcgg gtggtttccc tgaattgcgg attcaccaga
     1441 gaacgggtcg gatcgtaacc atcgtatgtc agtcccgcca aatctgacca ggtgtggatc
     1501 agttcagcca gactgtattt gcggtcaaca tactgcgaaa aatctcgcgg atgtgcagca
     1561 tgccactttt ccgaggtcca cagcaggaac ggaacagtgt acatgggacg tgtggggtta
     1621 tcttcattcc gtccctgcgt tttatatggc ggagtatcat atacttcttc accatgatct
     1681 gaaaaataaa gcagaaagcc gtccggcgca gttgctctga agtcctttat cagactggcc
     1741 accacatgat cgttaaacag attggcatta tcgtaatcgt tatacacttc cagctctttc
     1801 gcatttaatc cagtgggaat atgccctgta atgccatcaa accgcccctg accttccgga
     1861 taacggtatt tatacttaat gtgcgtaccc agcagatgca cgatgatcag tttctttggt
     1921 gcagggtcat tcagcacttc ccggaacggc ttcagcacgt tagtgtcata ttcacgcgca
     1981 ctttgtgttc gctgctgatt catgtagtac tgcctgtccg tctggcgcga aaatacagtg
     2041 agcatggtgt tacgggctgt gattgtctgc tggttggtaa tccagaatgt tttatagcct
     2101 gcctgcttca tcatgttcat cagcgacggc tgcgtcagat acagatcagg attcttttcg
     2161 ttggcgaaag taagggcctg ttgcaatgct tcaatggtgt acggacgcga tgccaccaca
     2221 ttattaaaca cagtaagacc cggatcggtt ttacgcagtg catccagctc cggcgtcgtt
     2281 tcacgtagat acccgtataa gctcatgcgt tcgcgctgtg tcgactcacc aatcaccagg
     2341 accagtgtgc gtggtctctc ccctgattca tccctgagat tacccagtgg tggcagtgcg
     2401 ctattttcat tcaggaaggt tgtcagtgca ttcagttgct gatggtactg ataataactg
     2461 gatacaaact gccagggggc agcaggctcc attcgggatg ccagtttgcc tagagtatca
     2521 ttcagcggct cctgtctgat taacgatttc agaacaaccg gatgcagaag cagagcataa
     2581 agcagcagga aagagacaat tctccgccat ggcaaaggaa tatatacagg acgcagacgt
     2641 gtccacagaa aaacggacac cgcagtatat accagcgaga taagcaacag tttaaggctg
     2701 aaatactggc tgaaatattc accagcctct ctggcattcg tttcaaacat aacgaaaaga
     2761 acactttgag agaattcatg accatagaga aaataataac aaagtgctgc cagcgacgtg
     2821 ccccagagaa tgaaaccgac aacagctgca attattttta tccgatcagg atagagaaac
     2881 accgggatca accacagaca actgaataac agtgagtccc gtattccatt cgttccgctg
     2941 tacccactgc tgaagatgat gacctgcagg agagtggaaa aaaagccaaa ataaaaaatt
     3001 gcccatccca gcgcacacca gctgaaagta ggcctgttct gtccggtatt taaatgcatt
     3061 gaccgtcccc gtatttaaac aatgtgataa attactccgt tacttaccgg aaaaccgctg
     3121 aacaaaattc gggctgaaaa gaggatccgc cgttatctgt tacatttccc cttagcctga
     3181 ctagccagag acacaatgat ctgtgccgtt ctgttaatat caaaccggta ctcaatatct
     3241 tctctggcgc tggctgccat catccggaag cgttccggtc gggataaaaa atcgcgcagt
     3301 gcgccggtcc atgcagacac atcccccacg ggtaacagcg tccctgtcac attcttctga
     3361 atgacatcag ggatcccgcc cgtctcactg gcgataacgg gcacgccaga gactgacgct
     3421 tcagccagta ccataccaaa cgcttcattt tccgaaggca tgaccaccac actggcaatc
     3481 cggtagaccg gtaacgctgg gaaaagggca cctgccatta acacatctcc gctcattccc
     3541 aggtgttctg tctgctgacg cagacgtgct tcgtattctt cacgcccggc gcccaccacg
     3601 agccagcgaa atgatttccc ttccatcttc agctgataca atacacgcag cataaattca
     3661 tgtccttttt cgggacgtag catccccacc tgaacgataa gcggaacatt gtctgctgat
     3721 gcagcccagg cgtggatatg caggggtaac ggtcgcatgg cttcattatg caatgcgggc
     3781 cagtcgaaac ccggtggaat aaccgttacc ggtgtcctga caccttccgc catcagatgc
     3841 gccatcatgg ctgagctagg cacaacaatg aaatcacaca gataattcag ggaaaacgtt
     3901 cgggtcttac gggtgatgta ggttttttgt ctgacaatac tgaagcggtg acagcatatc
     3961 agacggctca gtcctgctat attactgtca tggccactat ggcagatgac cagatcaggt
     4021 ttaaattccc cgataatccg tcgaagtctg aggatggaag gaaggtgaag gctgttcctg
     4081 aaaggaataa aagtgacatc atgccctctt tttctggctt ccggagcaat tttacttttt
     4141 tccctgcagg caagtaaaac ggaatgtccc tgcttttgaa gagcagtcat ctgggccagt
     4201 gcctgcagct cctggccccc aatatctgac gaagattcag tgaataaaat tttcatcatt
     4261 aattatctgg taatcttggc cccttatgaa gagccagtac tgaagaaaaa agtggcgttg
     4321 tataataaaa aaggcgtcgt ttcagccagc cggaatgttc ccgctctttg gtgctgatac
     4381 gccctatgcg taatgaacca ttttcaggac agttcattct tctttcggtt gtatataaat
     4441 aagagaaacc aagcttaccg gcgagattaa tataatcccg attataatat ccttcaggcc
     4501 agcacagatg ggaactacag aaccccagct tttccgtcag acattgtttt cccaccagaa
     4561 tatcttcttt catgagccgg cactgttcag cacgtgatac agacaacctg tcccagcgtt
     4621 tatgtgaatg ggtgtgcaca tgaaattcaa ccagtccgga atcacgcatt tcccggactt
     4681 cagaccagcg aagcatgacc tcatctgaac ggttatcggc aatcaaccgt tcacagtcgc
     4741 ggtgagaata ttcctgctcc tgcctgctac gtacgtttcc cttaccaatc agccccgtaa
     4801 tgagaaaaat atgtgcatga aggttatatt ccttcaggac cggccatgct ctgagccagt
     4861 tatcaagata accatcatcg aatgtgagca tgacactctt tcgtggaagc gttcccccct
     4921 gataaaaata ttcaagctca gcagatgtta ccgtccgcca gttattatct gcgagccact
     4981 tcatctgttc acaaaatgtt tcaggtgaga gtgtcacaag ccccggacaa cgactgacat
     5041 gatgatacat cagtacggga agatgccttg cattcaacat aaaaaattat cgtctgttaa
     5101 gcattctgaa aatatcctcg cgtaaacaac agtgaggatc catagatatc acacatggtg
     5161 atattattgt gtaatcccgg aatggtccgg agattaccga caacagggat tttttaatat
     5221 ttttatcagt taatcaacca gaacttaaat ttcccttaac gcatatgctc tttttaatca
     5281 gattttctgt ttttcagaaa aaacagaata ccgcaatttc agtaaacaca gcgagagata
     5341 tccgcctcag taaaaaatca gaagattatc atcctgattt attgcataaa accagtcgag
     5401 tagcggaatt ttctgcatcc ggataccact ccccctgaaa aatactcgca caatatcgcg
     5461 ccgcatgcac tgatggaccg ggccatcgtg agaatctctt gcatcgtaga tattaaccac
     5521 accagtgccc cccagaacaa cagtaatacc tgtagcagac ctgccataga ggttaaataa
     5581 ccattcatat ccctcttcta cccattcatc ccgaagtttc agccagcgac gaatggactg
     5641 tacctcctgt ttcagccgag gggctgagtt gacaaaccgt ttagagccgc cggatattca
     5701 ggttaccggc ctgcaaatca atatcagaca gttttatacc taataactct gatgcttgga
     5761 caccatgaag ataccccata aggagcaggc agtgattacg ttccgggtgt ggcactttga
     5821 agacagcact gtgcaactta tgaatctcac ccgggttcag atatttttgt tgtccattac
     5881 ctgtccttat ttattgaaag tcgatattag tttaaaaagc tgctaatcat gacaccatta
     5941 cagaagtaaa attaatttat tttaaataca taaaattatt gttcatttat tttttgcgaa
     6001 cattcataaa ctaaaaaaac aatggataaa accaataaca ttgcataata atacacctcc
     6061 cttataaata atggagaaga aaatgaaaag gcggtatatc acctatgctg aacctgttta
     6121 ggatgctgtg taaatacctt ttctccgaag tgaccgtcca agcggtcacc gaattcgata
     6181 ataaagcggc tcattgccat acaccagtcc ttcaacggca tcgtccattt ctgaggcgca
     6241 gactggatag ctaaagtgta gcggtattag gagctgttat ttagttcgaa tatcgaaaat
     6301 ttttgtgtac gttggtcgta tcaatctctt tttatttgaa ttagtttgat aacagatttt
     6361 tgcaatctga taactcattt atttattgag ggataaatgc aatattatac aatatgtttc
     6421 cgccattagc aaaagaatcc acaaggcgct tgcactaatg catggtaatg cctccaatta
     6481 gttgaattag ggg
//
LOCUS       DAZGTR010000198         6458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGTR010000198 DAZGTR010000000
VERSION     DAZGTR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.200"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(163..1428)
                     /locus_tag="ACHLCY_004614"
     CDS             complement(163..1428)
                     /locus_tag="ACHLCY_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371707.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMIAHSQPLMEALGKAIEAGDQEAATRLFGQLPKGHPLILMSNEGYAAKA
                     GQAVINLQKIFGDKPDMAIDSLNTPENLDVLSGVFAPELQQRIGMPDSTGDKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            complement(1439..1690)
                     /locus_tag="ACHLCY_004615"
     CDS             complement(1439..1690)
                     /locus_tag="ACHLCY_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP0371708.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            complement(1700..2146)
                     /locus_tag="ACHLCY_004616"
     CDS             complement(1700..2146)
                     /locus_tag="ACHLCY_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371709.1"
                     /translation="MEYGKYETLARAGYSGADRPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            complement(2149..2805)
                     /locus_tag="ACHLCY_004617"
     CDS             complement(2149..2805)
                     /locus_tag="ACHLCY_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371710.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            complement(2899..3300)
                     /locus_tag="ACHLCY_004618"
     CDS             complement(2899..3300)
                     /locus_tag="ACHLCY_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371711.1"
                     /translation="MAPVSGERMNDKILWYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTHLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            complement(3357..3497)
                     /locus_tag="ACHLCY_004619"
     CDS             complement(3357..3497)
                     /locus_tag="ACHLCY_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0371712.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            complement(3730..4464)
                     /locus_tag="ACHLCY_004620"
     CDS             complement(3730..4464)
                     /locus_tag="ACHLCY_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0371713.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGEHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKTSLAWAAGAQFNLNESVT
                     LDVAYEGFGSGDWRTSGVTAGIGLKF"
     gene            complement(4555..5172)
                     /locus_tag="ACHLCY_004621"
     CDS             complement(4555..5172)
                     /locus_tag="ACHLCY_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371714.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            complement(5178..5456)
                     /locus_tag="ACHLCY_004622"
     CDS             complement(5178..5456)
                     /locus_tag="ACHLCY_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HFP0371715.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINDNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(5471..>6458)
                     /locus_tag="ACHLCY_004623"
     CDS             complement(5471..>6458)
                     /locus_tag="ACHLCY_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0371716.1"
                     /translation="PNYRGHSLTEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGF
                     YWIRFVNAAGVKGPWNAEKGTQAQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQG
                     QAVKDAAIKTTEVVGTLREETTRTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGG
                     EAFLAMWSKKAGVDGITAGIGIVAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPF
                     AVSGGKVVIPKAMIYDAVIETLVSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQ
                     GNLNIGGLFSVTSQGQLTIRYSNQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
BASE COUNT         1560 a         1812 c         1341 g         1745 t
ORIGIN      
        1 gctgtcagtt tcgccgggtt gctgaatgtt caggctgtta cgattctggt taccgagttg
       61 cgcctcagga cgctgttcct ctgaataggc catacagact ccataaaaaa acccggcaca
      121 atggccgggc atcaggaaat ggtattaatg gaatatcgtg aattactggg cgtaattctg
      181 tttcagtcca cggataaact gagatgcaga tgaagcactt tcatctctgg cctgttcccc
      241 cctggccttt tgaatacgga gaaaattcgc atacccgttc tccagcaatt tctgattctg
      301 aggttccagc atttcaggct gacgggccgc tacatttctg gcaaatgaca gcttatcagg
      361 atcatctccc gcccagttga taacctgttg ctgaagtttt tgttgctgaa ttttctgttg
      421 atgcggttgt gaaccagtcg cagcataata ttcatccacc gcagcctgag cattaccgcc
      481 attcttgatc gcgtccgccg ctacgttgct ggcccccttc tgaagctctt tcagagaaag
      541 tccctgctgc ttcggcataa aataaccata atttttcgat atctctgcaa atttgctgcg
      601 atccctgacc tgagcgatag ccttatcaac gggtatcgcc agcacggttt gatcatcagg
      661 gtgcgcactg ccgtattctg ttacaggttt atgcgcggtg gagccatcgc tgtatgtgag
      721 atcaaggcca ataagtacgt acccttcctg ctgcgctggt acgatactgc caatcctggc
      781 ctcttttatc gttttgtccc cggttgaatc aggcatgcca atacgctgtt gtagttccgg
      841 ggcaaacacg ccggaaagca catcgagatt ttccggggta ttcagcgaat cgatcgccat
      901 gtccggctta tcaccaaaga ttttttgcag gttgatcacg gcctgacccg ctttcgctgc
      961 atagccttcg tttgacataa gaattaatgg atgtcccttt ggcagctgcc cgaacagacg
     1021 cgttgcagcc tcctgatcgc cagcctcaat tgcttttcca agcgcttcca ttagtggctg
     1081 gctatgggca atcatatcgt tatactcaag ccgtcgctgg ttgtatttct gcaactggag
     1141 tcgctgctgc tccataccca gtgacgcatt ccggtaattc tggttagcgt taaactccct
     1201 ttccttcagt gcataattac ggtcgtcaac tttggctctg taatcaaagt tccgctgatc
     1261 aacgttttta ttatgttcaa actgagactg cgcaaactca aaatcgcgct cgttattttt
     1321 ctgttgccgg gcaagctgtg cttcacgcag accaagctcc ttacgacggg tcattgcctg
     1381 gtcaacggtg ctgaatccgg caagtaaccc ctgtgcaaat ccgctcattc accactcctt
     1441 aaaacaaaga accagcaatg ccgccaatta ctgctccagc aacagcacca acaggaccac
     1501 caacggatgc gccaatagcc gcccccgtac caatgcccgt accgatattc tgcttgttct
     1561 gcgctttctg ttgcgccgcc atctgtttgt tcgcagcctc aatttcttca cgtcgtctgt
     1621 ctgcgtcact tattccctgt aatgcctcac gccgcgactg atttgcaata tccagtaaac
     1681 cgtatcccat attgccccct tacgctgcca ccagttggcc gccaacactc agtttctgtc
     1741 ttgccggtgc agaagccccc gtcagaatat tcatctggcg atcctgttcg gcttcacgga
     1801 taccattttt cgcgccagca attgccaggg cattacgtaa ccccagcgta ttactgtcgg
     1861 gattatccgg gcggtttacc ccgtatctcg ccatctggtt atcctgcgcc atctgcgctg
     1921 tacggagact ggaagtggca aggcctccca cccgtgcaag ctgtgcattc atcagactgt
     1981 tgttctcccc aaggtcagcc agccttgcca cgcggggcaa atatctggtt cgccagtcgt
     2041 cgtattgttg gcgtgtcagc gctgctgacg tctgccagtc accctgtggg cggtctgctc
     2101 ctgaataacc agcccttgcg agagtttcgt atttgccata ctccataatt acagtctcca
     2161 gttctgagcc tgatgctgaa tggcattagc gccggtgcca ggtgttttag caccgccact
     2221 tcctccgcta ccgccagcct tatgcatcgc atatgcacct gccgcaccca gaccagcgcc
     2281 aacaagagag gctcgcccct gctgtttcgt aaacgccgcc tgtgcatccg atttagcttt
     2341 tgccagactg ctgtctgcca gagagttaaa actctgtaac gcatccgcct tctgaccaga
     2401 accgagagca gcaacatcct gtagcccggc aacatactta tctgcctgcg atacctgccc
     2461 ccgtgtggtt gtgtcaatct gcccggttac ctggtcactc tggttcgcat tcattaccgc
     2521 attaaaacga ccactggatg ggtcaacgcc ggactgagca agattacccg caagctcctt
     2581 ccgtgcttcg ccaaactgtt tctgataccc cagatttgtg gtgccagcaa tattgtcgta
     2641 ctgctgctca ctgttcaggt catcgacctt ttccatgaag ttatcttcag ccgggcggag
     2701 gatatttttg taatcctgcc accctttcca ggccacttct tcctgtgcta tttgcgctgc
     2761 tgttggtttt actttggtat caccaccgcc tttacttcca cccataatgg ccccctggat
     2821 aacaaaaaac cctgccggag cagggtcaga atgtgaatta caatgttggt ttaaacacaa
     2881 tgatgaaaac tgtagcagtc agaccgctat cctgaacacc atgaagccat cctcatcatc
     2941 cggcattcgc tcaaagccca gtcgttttcc cagccggata aatccccgcc ttgccgtatg
     3001 gaactcagcc cagcgtccgc cagccagatg ggttaatgtc ttcacctccg gcaggtaacg
     3061 ctcaacgctg ttactccccg tacacacgcc cagcaacacc agaacataag ggataccatc
     3121 agcactgagc acagaacgca gcaccaaaaa gccatcaggt gcctcaaaac aaaacgcctg
     3181 ctttttaagg caggcgtctt taacttcatt cataaattca gggttgcggg aatttctcac
     3241 aacacgctgc atataccaga gaattttatc gttcattctc tcacctgaaa cgggtgccat
     3301 atcggctgaa cctcagcaac cagttgacgg ggactctcgt ccccgtcgcg gttttcctac
     3361 tgcttacact gtaagaacgc cgcaaactcc gctccccaca aattcagccg gaactcacac
     3421 agtgaaccgt gcaacatcca gatggtgaag ataaccgtca tgcaaattgt gacggtgata
     3481 agcgattttt gcgacatagc gcttgactcc ttttacagag aggcgctaac cttctacttg
     3541 cttaaggtac gaagtgttag ggcctcgggt taacgttaag ttgactcggg gcctttccac
     3601 atcaggcctt caggttcacc ctccagccat cagccgaaag gcacccacgc ataatttacg
     3661 gtttttgccc tgtacgggca ataaaaaacc cgccatcaca gcgggtagta aacggttacc
     3721 ggatacaggt cagaatttca ggccaatgcc agcagtaacg ccactcgtgc gccagtcgcc
     3781 agagccgaaa ccttcgtaag ccacatccag tgtaacactc tcattcaggt taaactgtgc
     3841 acctgcagcc cacgcaaggg aggttttttt cgtgctgttg ctttcagaaa atccgccact
     3901 actgttaata ttgtccttaa ttttcaggtc agcgctaact ttagcaacgc ccatccctgc
     3961 catcgcatat aaactcatgt actgattaac ccgccatgaa ggccccgcta acagactcca
     4021 gtaattcgcc ctgatatccg ttctggcaga agctgccgga ttcttaatct tcctggtatg
     4081 atcggctgac tgcacatcaa taaatgtctg tgagttagtg agagaacgcg tccatgtaaa
     4141 agaggtgata acgccaaaat catccgttat ctcgtagcga tacctgatat taatgccctg
     4201 tggattttta tccttgcctt catatccact gacccgacca tctgtatatt cgcccaatga
     4261 agaaaagtag tttctgttga cgaaatgact gaaagtctcc ctgttatgag cagtcgcatc
     4321 ctttacaaaa tccttcagtc ccggaaactg aaagtgcgca taccccagag aaattgtatg
     4381 ctctccttct gctgcatgtg cagataaatt tacacaggca atcccggaga ttgcacacac
     4441 aaccagtgtt gctatactct tcatttaaga tatcctgtat tcagataaca cgctaaactt
     4501 tttaataata catcttttta gacattttaa atgaatatta aagactcgct tcaactaatc
     4561 atatactgat gtattaataa ccaggacctt atctatatac ggctttcgaa tatcccatgt
     4621 tgcaccaccg gaataatgct cacaggaata tattttattt cctgtagcac cagtggatgt
     4681 ggtataaatc ggtcgatcat aaggagttct tttccagtta taatacccga ccagtgcagg
     4741 catgattgcg catggatatc caaggtcctt ttcaaatttg atatcaacag gtattaactt
     4801 cgcgtcaagc agcatcattt cgccatgata aatcatctca ccgtccgggt tatacatggc
     4861 gataccatac tcagaaggtg gagtaaccat attcgcgaaa gcataaaccg tcgtaacacc
     4921 agggttcgtt cccctgacaa ttacatgaag ccttagtgca tgatatccat caatctgttc
     4981 atgcgtgtac atgacatcgg ccttcttctc tgttctgata aagaaaaaac aacttttgcc
     5041 tgacgggatt gatgttttaa aaaaagactt ttcagtcgcc ggtatggtgc ctttgttgat
     5101 caaacactgc ggcgtaaaac ctggacttat ccatacgctg ccatccggct tcataatgct
     5161 caaaccgtac ataacactta tccccagaat gtataaatat aagatcccat accctgctca
     5221 agattcgacc acgtcaccgt attgtcatta atggttatct tcggtacttt ccgatccgca
     5281 aatacattat tccagggaaa taaacaacat acagcctgca atgatttccc gtcgggttta
     5341 ttagtgtacg tctttgagcc agactccgct gtaaatctgt ccaggaaaaa tactggagtc
     5401 agcacgcccg taacattaac gttatttctg ttataaatgg caaaaccgta ttccaatact
     5461 cacctcctga tcagcgtaat ctgcctatgc gaacagccag tcgtccattc tggtcataaa
     5521 cctcaatttt atcattgcgg atcaccagtc ctacattctg attagagtaa cgaattgtca
     5581 gttgcccttg tgacgtaaca ctgaaaaggc ctccaatatt caggttaccc tgagaatcaa
     5641 cctgaaagtt tccgttctga ataacggcac tccggataac tggcgaagtg atacttaccc
     5701 cggcttttac ctcatccgcc acaaccttcc gcgacaccag tgtttcaatc accgcgtcat
     5761 aaatcatcgc tttcgggatc actaccttgc cacctgatac cgcaaacgga taggctgtgt
     5821 tatccggatt attcgggtca aagacaaaca actgcgacgc agaaattgca acctgactta
     5881 caggcctgcc ttcactgtct tttccggcga caatcccgat ccccgcagtg ataccatcaa
     5941 ctcccgcttt ttttgaccac attgcaagaa aagcttcacc gccttcttta tccaatttag
     6001 tgatgcgctt gtcgacctca ttaagcgatt cactggttga cgaatccagt gtgctaatgc
     6061 gggtttcaat accaccaatc gttcttgtcg tttcttccct gagagtccct acaacttcgg
     6121 ttgtcttaat tgcagcatcc tttacagcct gcccctgcgc gttttttatt tctttacgca
     6181 gctcggacac aaccggcgac tttgcagcct catcgcggat ctggtcaatg atggccttca
     6241 cgccgatctg tgtttgtgcc tgagtgcctt tttcagcatt ccatggacct ttcactcctg
     6301 ccgcgttaac aaaacgtatc cagtaaaatc ccgaccagcc agggtcaacc ggatcgccgt
     6361 aaacctgccc cggcgtcgtg gcaaccagca ctgcatcagc aaggtcatcc tccgtacccc
     6421 gccagatttc agtcagtgaa tgtccgcgat aattaggc
//
LOCUS       DAZGTR010000199         6407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGTR010000199 DAZGTR010000000
VERSION     DAZGTR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.201"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..112)
                     /locus_tag="ACHLCY_004624"
     CDS             complement(<1..112)
                     /locus_tag="ACHLCY_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371717.1"
                     /translation="MREQIKQDIDLIEILFYLKKKVRVILFIIAICMAMML"
     gene            complement(325..1995)
                     /gene="betA"
                     /locus_tag="ACHLCY_004625"
     CDS             complement(325..1995)
                     /gene="betA"
                     /locus_tag="ACHLCY_004625"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP0371718.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRI
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2009..3481)
                     /gene="betB"
                     /locus_tag="ACHLCY_004626"
     CDS             complement(2009..3481)
                     /gene="betB"
                     /locus_tag="ACHLCY_004626"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP0371719.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGGGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3495..4082)
                     /gene="betI"
                     /locus_tag="ACHLCY_004627"
     CDS             complement(3495..4082)
                     /gene="betI"
                     /locus_tag="ACHLCY_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP0371720.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4211..6244
                     /gene="betT"
                     /locus_tag="ACHLCY_004628"
     CDS             4211..6244
                     /gene="betT"
                     /locus_tag="ACHLCY_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP0371721.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1333 a         1752 c         1748 g         1574 t
ORIGIN      
        1 aaagcatcat tgccatacag atagctataa taaaaaggat aacacgaacc tttttcttca
       61 gataaaataa aatctcaatc agatcgatat cctgtttgat ttgttcacgc ataatatatc
      121 cagaaaataa aatctgtcgc agataaggtt gtattaatag tctgtatcag gaatgttcgg
      181 gttaaatatc agcaaaaagc cagcatcatg gatactggat atgaagcatg agagttacct
      241 cagtgtttat ataaggattc ggtccccctc tctggaacgg taactctcaa tctgatcggt
      301 tcctgcgtta gttcacatca cgactcattt tttcgctctc accggcatcc catttgccac
      361 aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca cgaatcatat ccgctatttt
      421 ctcgccaatc ataattgtcg tggcgttcaa attcccggtg ataatctgcg gcataatcga
      481 cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg ccttcgccgt caaccacgga
      541 catctcgtcg taacccattt tgcaggtacc gcacggatgg aaggcggttt cggcgtggtt
      601 acgcacgaac tcatcgagct gttcatccgt ctgacattcc gtgccggggc tgatttcgcg
      661 gccacgatac tgatccagcg cgggttgatg catgatctcg cgggtgatgc gaattgcgtc
      721 gcggaactcc tgccagtcct gctcgtgcga catgtagtta aacagaatcg ccggatgctg
      781 gtgcgggtcg cgggatttaa tccgcacatg cccacggctt ggcgagcgca ttgagccgac
      841 gtggcactgg aaaccgtgct ctttcactgc attcgagccg ttatagttaa tcgctactgg
      901 caggaaatgg tactgaatat tcggccacgc aaattcctca cggctgcgaa taaatccacc
      961 tgcttcaaag tggttgctgg caccaacgcc agtgccgcca aacagccact ccgcaccgat
     1021 tttcggctgg ttccaccact gcagggcagg gtagagggaa accggttctt tgcactcata
     1081 ttgcagatac atctccagat gatcctgaag attttcgccg acgccgggta attcatgcac
     1141 cagcggaata tcaaactccg ccagcagttc agcgttgccg acgccggagc gttgcaggat
     1201 ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct ttgttggccg ttgcgcgggt
     1261 tgggatggtg ctgtcgcctt ccagccattc gacgcccacc gcgcgtttgc cgtcaaaaat
     1321 gatgtgatcg gtcatagcgt gaatacgaat ggtcaggtta gggcgcgatt tggcctgatc
     1381 gagataaccg cgcgcggtac tggcgcgacg gccctgcggc gtgacggtgc gatccatcgg
     1441 accaaaacct tcctgctgat aaccgttgag atcgtccgtg cgcgggtagc ccgcctgcac
     1501 gcccgcttca atcatcgctt caaacagcgg attgacgccg ggtttggagg tagtgacgct
     1561 caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca tcgcgagtct cggccttgcg
     1621 gtagtagggc aggcagtcga gataactcca gttctccaga ccgggttctt gcgcccagtt
     1681 atcgagatcc agcgcattgc cacggatgta gcacatgccg ttgatcagcg acgatccacc
     1741 caggccttta ccgcgtccgc actccatgcg gcggttattc ataaacggtt caggctctgt
     1801 ttcataggcc cagttgtagc gtttgccctg tagtgggaac gccagcgctg cgggcatctg
     1861 ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca agcagcagca cggaggtatt
     1921 cggatcttca gtcagacggg tagcgagaac gttgccggct gagccggcac caataatgat
     1981 gtagtcaaat tgcaaataaa cctcctggtt agaatatgga ctggaattta gccatctcaa
     2041 cctggatgga cttcacctgg gtgtaactct ggagcgtcat cacgccgttc tcgcgaccaa
     2101 tgccggagtg tttgtagccg ccaacgggca tctctgccgg ggattcgccc caggtgttga
     2161 tccagcaaat acccgcttcc agctgatgaa tgacgcgatg cgcgcggttc aggtccgccg
     2221 tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc gcggcgaatc acttcgtcct
     2281 cagattcata agtgagaagc gacatcaccg gaccgaagat ctcttcgcgc acgatggtca
     2341 tttcgtcgcg gcaatcggtg aacacggtcg gtgcaaccca tgcgccgtta tcgaagccat
     2401 cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc ttcctctttg cctttggcga
     2461 tatagcgcag cacgttatcg cgatgcggga agctgaccag cgggccgaag ttagtttgcg
     2521 gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc cagaattttc tgctcaaatg
     2581 cggctttgca tttcgccgga acgaagacgc gggtgccgtt ggtacacacc tgaccggagc
     2641 tgaagaagtt cgccatcatg gcgatatcgg cggcgagatc gagatccgca tcgtcgaaaa
     2701 cgatcagcgg tgatttaccg cccagttcca tggtcacttc tttcagggaa gaggccgccg
     2761 agttagccat cacttttttg ccgctggcga caccgccggt aaatgacact ttggcaatgc
     2821 ccggatgctc ggtcagatat tgcccggtct ccgcgccccc ccccggcaac acgttaaata
     2881 cgccgtccgg caggcccgct tcgctgtaaa tttcagccag ctttaacgcg gtaagcgggg
     2941 tgacttcgct cggtttgaaa atcattgcgt tgcctgccgc cagcgccggg gcggatttcc
     3001 acagggcaat ctggatcggg tagttccatg cgccaatccc cgccaccaca cccagcggtt
     3061 cgcggcgggt ataaacaaat gacgtctcac gcaacgggat ctggctgcct tccagcgccg
     3121 ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt aacgatatcg acggttgagg
     3181 tttccgaata tgcttttccg gtatcgaggg tttccagttt tgcgagttcg tcattgcgtt
     3241 cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg ctccatagcg gtcatcgccg
     3301 cccagatttt ttgcccctgt tgggcactct tcacggcgcg atcgacatcc tcacgcccgg
     3361 cggcctgcac ggtcgccagc acgttaccgt tggccgggtt aatggtctcg aaggtgcgac
     3421 cgctggtggc ggaggtataa ccaccatgta tataaagctg ctgttctgcc attcgggaca
     3481 tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa taaagtgacg ggtcagcgag
     3541 tgtgcgagag gtttatccag cggtttgccg ctcagtgccg cgcgcagcca taatccgtca
     3601 atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt ggcgcggtaa ctcgcggcga
     3661 aattcgctca ccagattcga caataagcgg cggctactga cctgctgtaa acgatagagc
     3721 atcggctgat gcatactgct ggcccagaac gccagccagg ctttcatcgc cgcactgctc
     3781 acctgcgttt catcgaagtt tccgccaaca atcgcctgta atcgctgctc tgcactgccc
     3841 tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca gctgactggt gatatcgcgc
     3901 atggttgctt ccagcagacc atttttgtcc ctgaaatagt ggctgatgat ccccgtagaa
     3961 acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt gcatgcccac ttcatttatt
     4021 gcttccagtg tggcgtcgat cagttgtctg cgccggatcg actgcatccc caatttgggc
     4081 attttcgcca ctccattcat cagcgttgtt tatctattaa agcggttatt gattggacgt
     4141 tcaatataaa atgtgtctta attgttacga atttgatttt aaatggtaac aataacagtg
     4201 gggatactgg atgacagacc tttcacacag cagggaaaag gacaaaatca atccggtggt
     4261 gttttacacc tccgccggac tgattttgtt gttttccctg acaacgatcc tgtttcgcga
     4321 cttctcggcc ctgtggattg gccgcacgct ggactgggtt tctaaaacct tcggttggta
     4381 ctatctactg gcggcaacgc tctatattgt ctttgtggtc tgtatcgctt gttcgcgttt
     4441 tggttcggtg aagctcgggc cagaacaatc caaaccggaa ttcagcctgc tgagttgggc
     4501 ggcgatgctg tttgctgccg ggatcggtat cgacctgatg ttcttctccg tagccgaacc
     4561 ggtaacgcag tatatgcagc cgccggaagg cgcgggacag acgattgagg ccgcgcgtca
     4621 ggcgatggtc tggacgctgt ttcactacgg cttaaccggc tggtcgatgt atgcgctgat
     4681 gggcatggcg ctcggatact ttagctatcg ttataatttg ccgctcacca tccgctcggc
     4741 gctgtacccg atcttcggta aacggattaa cgggccgata gggcactcag tggatattgc
     4801 agcggtgatc ggcaccatct tcggtattgc cactacgctc ggtatcggtg tggtgcagct
     4861 taactatggc ttgagcgtac tgtttgatat tcccgattcg atggcggcaa aagcggcact
     4921 gatcgccttg tcggtgataa tcgccacgat ctctgtcacc tccggtgtcg ataaggggat
     4981 tcgtgtgtta tcggaactta acgttgcgct ggcgctggga ttgatcctgt tcgtattgtt
     5041 tatgggcgac acctcgttcc tgcttaatgc gctggtgctg aatgttggcg actatgtgaa
     5101 tcgctttatg ggcatgacgc tcaacagttt tgccttcgac cgtccagttg agtggatgaa
     5161 taactggacg ctcttcttct gggcatggtg ggtggcatgg tcgccgtttg tcggcttgtt
     5221 cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc gtgctgggca cgttgattat
     5281 tccgtttacc ttcacgctgt tatggctctc ggtgttcggc aatagcgcgc tgtatgaaat
     5341 catccacggc ggcgcggcat ttgccgagga agcgatggtc catccggagc gcggcttcta
     5401 cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca ccattactgg
     5461 cctgctgttt tatgtgacct cggcggactc cggtgcgctg gtgctgggga atttcacctc
     5521 gcagcttaaa gatatcaaca gcgacgcccc cggctggctg cgcgtcttct ggtcggtggc
     5581 gattggcctg ctgacgctcg gcatgctgat gaccaacggg atatccgcgc tgcaaaacac
     5641 cacggtgatt atggggctgc cgttcagctt tgtgatcttc ttcgtgatgg cggggttgta
     5701 taaatctctg aaggtagaag attaccgccg tgaaagtgcc aaccgcgata ccgcaccgcg
     5761 accgctgggg cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc tgatgaatta
     5821 tccgggcacg cgttacacta aacagatgat ggagacggtc tgttacccgg caatggaaga
     5881 agtggcgcag gagctgcggt tgcgcggcgc atacgtggag ctaaaaagcc tgccgccgga
     5941 agagggacaa cagttggggc atctggattt gttggtgcat atgggcgaag aacaaaactt
     6001 tgtctatcag atttggccgc agcaatattc ggtgccgggc tttacctacc gcgcacgtag
     6061 cggtaaatcg acctactacc ggctggaaac cttcctgtta gaaggcagcc agggcaacga
     6121 cctgatggac tacagcaaag agcaggtgat caccgatatt cttgaccagt acgagcggca
     6181 ccttaacttt attcatctcc atcgtgaagc gccgggccat agcgtgatgt tcccggacgc
     6241 gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg gcgcgggaat cattgactcg
     6301 ctgtaaaaaa ttatgcatat attatatatg attgtcatgt catatcctgc aattcctctt
     6361 gtatcacttc atgattttat attttattaa ttaacgattt tattttg
//
LOCUS       DAZGTR010000200         6336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGTR010000200 DAZGTR010000000
VERSION     DAZGTR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.202"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..2027)
                     /locus_tag="ACHLCY_004629"
     CDS             complement(<1..2027)
                     /locus_tag="ACHLCY_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0371722.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFIPVEPESDPM
                     NVNFDKLSLEVQNAVLVKFGTCENITVDMAIDAQELLQEDVATFDGHIVEALMKTPEI
                     NAMYPERKLFAIGWVKHKCNPGAKWPEIQAELRNWKKRQDAERKETGKYTSVVDLASA
                     RVNQQHTENSAGKINPVTAAICREYKQTWKTLDEELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     NQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHS
                     IKVNGHQEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGP"
     gene            complement(2121..2312)
                     /gene="ydfD"
                     /locus_tag="ACHLCY_004630"
     CDS             complement(2121..2312)
                     /gene="ydfD"
                     /locus_tag="ACHLCY_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFP0371723.1"
                     /translation="MNSAFALVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(2309..2497)
                     /gene="dicB"
                     /locus_tag="ACHLCY_004631"
                     /gene_synonym="ftsT"
     CDS             complement(2309..2497)
                     /gene="dicB"
                     /locus_tag="ACHLCY_004631"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0371724.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            3000..3200
                     /locus_tag="ACHLCY_004632"
     CDS             3000..3200
                     /locus_tag="ACHLCY_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371725.1"
                     /translation="MTKEEFVSYIFDKTVEMYAATYGSCNPLNKPEGKDDFDKIYRFL
                     EDRYIKRLEDAGIKSPVKSPLS"
     gene            complement(3169..3534)
                     /locus_tag="ACHLCY_004633"
     CDS             complement(3169..3534)
                     /locus_tag="ACHLCY_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371726.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPGVLAGEMCTEISSELLPVIREAICRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
     gene            complement(3546..3698)
                     /locus_tag="ACHLCY_004634"
     CDS             complement(3546..3698)
                     /locus_tag="ACHLCY_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0371727.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            complement(3891..4298)
                     /locus_tag="ACHLCY_004635"
     CDS             complement(3891..4298)
                     /locus_tag="ACHLCY_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371728.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            4376..4603
                     /locus_tag="ACHLCY_004636"
     CDS             4376..4603
                     /locus_tag="ACHLCY_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0371729.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            4587..5108
                     /locus_tag="ACHLCY_004637"
     CDS             4587..5108
                     /locus_tag="ACHLCY_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0371730.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITESFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            5089..6054
                     /locus_tag="ACHLCY_004638"
     CDS             5089..6054
                     /locus_tag="ACHLCY_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371731.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GNELLDGGKLNESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRNPDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVNLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            6095..6336
                     /locus_tag="ACHLCY_004639"
                     /pseudo
     CDS             6095..6336
                     /locus_tag="ACHLCY_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
BASE COUNT         1594 a         1432 c         1543 g         1767 t
ORIGIN      
        1 tccggtccca tatctccggt ttgcggatcg aaaaatattg cacctattga gatgatcggg
       61 gcatcaggat tttttcccat ggtttcaagg tcgatcatta gatggtcaca cgtcctgctg
      121 gtggatgtga tttcttgatg accgttcacc ttaattgagt gatctgccgt ctcgccagtt
      181 tcattatcgc tattgtgatg ctgattgccg ccagtgttct ccttgtgtgg atgttcagcg
      241 ccttccattt cctccggatc atcttcctga acttcaacct gatattcttc atcgaatgtt
      301 tcttgatatg ttgcgtcgcc catcaccgcg ccacaatcag ggcagttgcc gccgccggtc
      361 tgaccgcagg cggtgcagac tttttccggt tcctgttgcg ctactggctc aggttgtttc
      421 gtttctggct cgttttgttg cgtatttggg ctgttttgtt ccgctttctg gtcgttctgt
      481 tccgattctt gctggttctg gtttacagaa tcgcgggttt caatcccctt tacccatttc
      541 ggatcattcg ggtcgctaat cccttcaaca aattcaccac gtgatgcagc aagcaactta
      601 tcggcgtcag gctggctgat attggctgcc tgcataattt tgtttacttc gtcagcggta
      661 acttttatcg gctctggttg ttctgaatct tcagcggtat ctacattttg cggtaagccc
      721 gtgtatgtgc cattttttcg ggcaaaatat tcttcttttg tgatttcagt ggcgccagca
      781 gccagtgcct tatccagacc agaaagtttg tttgcgcgac cgtatttttc tccgtcctta
      841 tctgcgaaga ggaaatagaa cggcccctca cgctctacag atggttcagc ttccggcgcg
      901 gtttcatttt ttgggatatc agatacctca gtttccactg catcagtttg tgtttctgat
      961 gactggagaa catcaacagt gcccaggtct gtttcttcat tctcaaacac gccctttgta
     1021 gtcaggtatt cagtaatgta tttgttcagt gccacagggt ctttgtgaat gtcgatcgga
     1081 cgttcacgga caaggccaaa aatagtctgg cggtcgtagc gaagggcatc aggctgtttg
     1141 cgcattgatg ccgagatacg cttccagtct tcgcggtcgt tgtcgataac ttcatttttt
     1201 gcccagcgat ggatgctgcc gtcaatgttt ccggcatcca catcaccagg ccagagagcg
     1261 taggccagtt cttcatccag cgttttccat gtctgcttgt attcgcgaca aatggcggca
     1321 gtgactgggt tgatttttcc tgctgagttt tcagtgtgct gttgattgac tctggcgctg
     1381 gcgagatcaa caacagacgt gtattttcca gtctctttgc gctctgcgtc ctgccgtttt
     1441 ttccagttac gtaattcagc ctgaatttcg ggccatttgg cacccggatt acatttgtgt
     1501 ttaacccatc cgatagcgaa cagtttgcgt tccggataca tagcgttaat ttcaggcgtt
     1561 ttcatcagtg cttcaacgat atgcccgtca aaggtagcaa cgtcttcctg cagtaattcc
     1621 tgcgcgtcaa tcgccatatc aacggtgatg ttttcacatg taccgaactt aaccaggacc
     1681 gcgttctgta cttcaaggga cagcttgtca aaattgacgt tcatcggatc ggattctggt
     1741 tcgaccggaa taaaggaagc ggattcctca tcccagcggt tttcctgcat atattcagca
     1801 tcccatgaat cgagggcagg gcggggtatg ccaggtttat cctcgcagac aataaattta
     1861 taagcgcagt cctgagcagc cggataatgt tccaggaact gccagtgaaa ttttgctcga
     1921 gcacggcgtt cgtcgccagc ttcaatggct gtggctacag ccacagcgcc ttcttccctt
     1981 gttgccagtt cgtcaggaat agcggcgcaa ataaagactt tactcatttt gttttaacct
     2041 catgacagat ttaaggatga acaaatccct gccattgctg gcatataaga atgaaaccgg
     2101 atatttatta cggaactgtt ttaaagacct gccgggattt cgatattatc ctggtgaata
     2161 actttatcga ccgggtaaca gttaccggga attttctgtt cggttgctgc agtcatacac
     2221 tcctgcattg tcctgtgaac actgactgca atatcaactg gctctccgga aacaagaaaa
     2281 actgtcagaa caagcgcaaa tgctgaattc attgtgcaca tccttttggc atcagacgta
     2341 aacgagccag cattgaaaca atgcatattt tatttaatag ctcccgttct tgttttctct
     2401 tgttaatggc atcttcagta aatacagggt tactgatagt gacaccaatt tcaaaacaac
     2461 cttcagacgt attaacgttt ggtaataacg ttttcattat cgcgtcctca acaatgaatt
     2521 ttgtgatgca gtgcctggtg cctccaggtg acgttaacca gttaacaatt aacgccggat
     2581 acagagaatc cacccataac actgtttttg gttttaactg ttccgcgtgc gcttagccgc
     2641 attcaccgca tcacaaaatt cactttaaaa acggcggcag agcagtcacg gagtaaaact
     2701 gataccgcca aacgtcacca gaaaattgat aacagagggc gttgcagcgg ggttgtcact
     2761 taagcgtatg gtcaacctga caacccggtg tcctcaacgg ggaaggaata accccgccat
     2821 acttaccgcc gcgccatttc gcgggttgcc acaaccggaa gcgcacggtc gaattaaatt
     2881 taacgacacc gtacagtgag acgaacttcg ccgtgcgctt tcgtgttgtg tgcctgcttt
     2941 taaccacgtc aggcgaggtg gtatccttct tattccgaat aaccaagaag gaaatctata
     3001 tgactaaaga agaatttgtc tcttatattt ttgataaaac ggttgaaatg tatgccgcta
     3061 cttacgggtc ctgtaatcct ctgaataaac cagaggggaa agatgatttc gacaaaattt
     3121 accgcttctt ggaggaccgc tatatcaaaa ggttagagga cgcagggatc aaatccccag
     3181 tgaagtcacc attgtcctga gaacttgcag gacgtcatga tcgtaacttc catccaaacc
     3241 gcgacggcaa attgcttctc gtattaccgg aagcagttcg ctggaaatct cggtgcacat
     3301 ttcacctgct aatacgcctg gctcaagtga gaatattggt gaacttacgg tcttggtctc
     3361 gacagtttca gagtcagtgc caacattata aagctcaacg aaagcggtct tgattttccg
     3421 ggccagatct tttgctggct cgcttgcaat atctttcccg atttctcgca gcacagaatg
     3481 caatgtatga gctgctgttt tctgtacatc agacggtaaa tctttaaatt ccatcgtcag
     3541 cctcatcagt cagtgtttct ggctaaccag caacgcgcgc cagattcggt tttaaacgtt
     3601 ttgcttttgg tatatgtcat cgcggtgaac gtaccgtcct ggttggggaa cacgccacat
     3661 accagagatt cgctgttgcc aagatcgata gtatccatgc tgacctcatt tccccttaac
     3721 gccggggtag cggaactgtt tgctgagaac accgtgcggt gtgttgatgg aggtaattta
     3781 gttttctcat tatttttcgt caagattttt tgatgagaaa actcaagtat tggcgcaaga
     3841 taaagccaat acattgaaat gtaaggcttt aaaattttgt gaagggatga ttattgatgc
     3901 ttgttgcgtt tgcgagcctc taatagctcg gtgaacaggc gattaaaatt ctcaacgcgg
     3961 gcgcggagtt cgctgagttg tgcttgttgc tctgatttag gaagtgcgcg atacaaacgc
     4021 agcatctcta actcatcttc cgataagtct aaggcactat cgagtgaaac agggggagct
     4081 ggtgttttgt cctcgtcgcc aaacagtatc caggttggtg aacattgcaa tacctcggcg
     4141 aggcgatgca aattttgccc acgcggggct gtatggtcac tttcccataa tgaaattgat
     4201 gagccagata cgccagcagc tttgcttaaa ccgttttgac ttaagcctac ctgcttacgt
     4261 ctttctctaa tgcgttgacc taaagttttc tcgttcatat ttagatatct taataaccct
     4321 tgacttgaga ttccttgagt gattagcttt gagaaaactc aatattggag gtgcgatgtt
     4381 taaatcagac gtaattaatt tttacggtac gaaagccaaa gtagcgaaag ctgctggcgt
     4441 tgacccatct gctgtttctc aatggcaaga gctggttcct gaaggtcgcg cgatgcgtct
     4501 acaggaggca tctggcggcg agcttctgta tgatcccaag gtttatgacg aatatcgtaa
     4561 gacgaagcgg gcggggcggt tgaacaatga aaatcactcc tgaacaggct cgtgaggctc
     4621 tggatgcctg gatatgtcga ccaggaatga cacaggagca ggcgacgata ttaatcactg
     4681 aatcattctg ggctttgaaa gagcgcccga acatcgatgt tcagcgtgtc acagatgaag
     4741 gtggcgcggt tgatcagcga gcgcttggcg ttaatcgagt gaagatattc gaacgctgga
     4801 aggctatcga cactagggat aagcgtgaaa agttcacggc gctagtgcct gcaattatgg
     4861 aggctatccg gattaatgat ttcaggttgt atcgtgaaat tagtgacgga aaaagcatca
     4921 cgtacatgat cgccgggtta aacaaagaat atggcgatgt ggtggagtcc ggactgcttt
     4981 ttgctgatcc tgccgtagtg gatcgtgaaa ctgacgaact tatagaaaaa gcaattgctt
     5041 tcaaacttgc gtatcgacag caataccaac aaaaagctgg atggaattat gagccttctt
     5101 tttgctgaac gcccactggt tataaacaca cagcttgcga tgaagattgg cttaaatgaa
     5161 gccattgtgt tgcagcagtt gcattactgg ttgagagata ccaattccgg catggaatgt
     5221 gatggtgttc gctggattta caacacaacg gaacaatggc tggaacagtt cccattctgg
     5281 tcagagtcaa cgttaaagcg cgcgtttgca agtctgaaaa cgctggggct tttgcgttgt
     5341 gaaaagctca ataaatcaaa gcgcgatatg accaatttct acacgatcaa ctatgggaac
     5401 gagcttttag atggtggcaa attgaacgaa tccatcggtt caaaatgcgc cgctccatca
     5461 ggtcaaaatg acacgatgga agaggtcaaa atgaaacgct ccattggttc aaaacgaccc
     5521 aatgtcatcg ggtcaaaatg gcctgatgat cttacagaga atacaacaga gattactaca
     5581 gagaataaaa acacttttcg tccggaagct tcgcaaccgg acccgcagac cgctgaacag
     5641 gattttttaa tccggaaccc cgacgcggtt gtgtttagtg cgaaaaaacg ccagtggggt
     5701 agcagggagg atttggcgtg tgcgcagtgg atttgggggc ggatcgtgaa cctttacgaa
     5761 caggctgcca gcgacgatgg agagatcatg cgaccaaaag agcctaactg gacagcctgg
     5821 gccaatgacg tgcgcacaat gcggatgctg gatggcagaa gccacagaca aatttgcgaa
     5881 atgtttggtc gggtacagcg agatccattc tgggtaaaaa acatcatgag cccgtcaaaa
     5941 ctccgcgaaa aatgggacga actggtcatc cgcttgggac gttcacctgt acagcgttgt
     6001 gtgaatcata tttctgaacc ggatacagaa attccgcctg gtttcagggg ataagtgttg
     6061 atttcaggtc atgaggtaat tttaaggagg acttgtggca aaagttttta cacaggaaga
     6121 gcgggaaaaa aattaaagag caggtggtcg aacttgtgcg ccagagcggt cgcgagacat
     6181 tacgacagtt ggaagctaaa acaggtgcga caagatatct gatgagcatt cttgccagag
     6241 agctggttgc cagtggttat gtatacaact ccggctacgg gttattcccg tctgaacagg
     6301 cacgaaagga ctggcaaaat gcccgcaaaa aactct
//
LOCUS       DAZGTR010000201         6303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGTR010000201 DAZGTR010000000
VERSION     DAZGTR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.203"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1978)
                     /locus_tag="ACHLCY_004640"
     CDS             complement(<1..1978)
                     /locus_tag="ACHLCY_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0371732.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWNDRENARLGLAAATLQSDMEKAGELAARD
                     RAERDASQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANGKISQQRRDL
                     WKAESQYAVLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQ
                     QAARFEQQQGAKQAAISAQARGLTDRQVQRESEEQRLRDVYGDNPDALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKS"
     gene            complement(1959..2372)
                     /locus_tag="ACHLCY_004641"
     CDS             complement(1959..2372)
                     /locus_tag="ACHLCY_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0371733.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
     gene            complement(2399..2830)
                     /locus_tag="ACHLCY_004642"
     CDS             complement(2399..2830)
                     /locus_tag="ACHLCY_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0371734.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTESPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(2844..3596)
                     /locus_tag="ACHLCY_004643"
     CDS             complement(2844..3596)
                     /locus_tag="ACHLCY_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0371735.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYNGQGDAYANPLSDDNWQRLAQVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(3604..3999)
                     /locus_tag="ACHLCY_004644"
     CDS             complement(3604..3999)
                     /locus_tag="ACHLCY_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0371736.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEALDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALSGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(3996..4571)
                     /locus_tag="ACHLCY_004645"
     CDS             complement(3996..4571)
                     /locus_tag="ACHLCY_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0371737.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPSASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPAIKLGIRMLGHRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPEARYAKGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVNRIRRERLPKELGYALKQQLRIVIKR"
     gene            complement(4586..4939)
                     /locus_tag="ACHLCY_004646"
     CDS             complement(4586..4939)
                     /locus_tag="ACHLCY_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0371738.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGAQRGGEVQGVFD
                     DPESLGFASSGIRIEGSSPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(4932..5306)
                     /gene="gpFI"
                     /locus_tag="ACHLCY_004647"
     CDS             complement(4932..5306)
                     /gene="gpFI"
                     /locus_tag="ACHLCY_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0371739.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEMQS
                     SGDDVQVMNMDIRERENAAHDVREETSGALTRIRVLTCLHLCGVDGETGESVELADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(5358..>6303)
                     /locus_tag="ACHLCY_004648"
     CDS             complement(5358..>6303)
                     /locus_tag="ACHLCY_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0371740.1"
                     /translation="RRTVNFHTEEVMLDKITGKTPVAAYVSPVVEGKVLRHRGGETRV
                     LRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLRIITDNLKQEEHAIVQVEEMQ
                     AVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNITQGSGKEWSKQDRDTFDPTHDLDLYC
                     DQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYG
                     DLAIVVAKTSYVAEDGTEKRYLPEGSLVLGNTAAEGIRCYGAIQDAQALSEGVVASSR
                     YPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT         1320 a         2011 c         1479 g         1493 t
ORIGIN      
        1 cgcttttaac ctgcgaaaaa ctgtccgtcg cactttctgc ccactcgccc cagccggact
       61 tcagaccggc catccagctt ccacgaagct gctcctccgc agaccaggtg ttcttcagtg
      121 cagatgtggc cttcgccagc gcatccggat tatcaccgta cacgtcacga aggcgctgct
      181 cttccgactc ccgctgcacc tgacggtcgg tgaggccccg cgcctgggca ctgattgccg
      241 cctgcttcgc gccctgctgc tgctcaaacc gcgcagcctg ctgtgccagc tcattcaggc
      301 gtttctggta ttcaaccttg tcgcccagct cagccagctg gcgtttgtac tccagcgtct
      361 cgtctttatg cgccagcagg gatttttcct gctcagataa ctgtcgtttc gtggcagcct
      421 ctttcaggac cgcatactga ctttccgcct tccataaatc gcgacgctgc tggctgattt
      481 tcccattcgc accgctgtgt ttttccagcg tccggagctc ggtttcaagc gccagcaggg
      541 cagcatgcgc ctggtcttcc tgacgctcac cggctgacac tttgactcct gacgacttcg
      601 gctttttcag cgtcgattca taatcctttt tcgccgccgc catcagcgtg ttgtaatccg
      661 cctgcaggat tttcccgtct ttcagggcct tattcagttc ttcctgacgg gcggtatatt
      721 tctccagcgg cgtcagcaga cgctcatacg ccttctgcgc ctctccggta tacttcagct
      781 gtgatgcgtc ccgttcggcc cggtccctgg cggccagttc accggctttt tccatatccg
      841 actgcagcgt ggccgctgcc agccccagac gggcattttc ccggtcattc catgcgccct
      901 gaaggttggc ccggaaagag gaggttttac cgcggcgctg gctccgactc tggtaccact
      961 gccatttttt atccgcctca tcaaatgcct tctgcgcact ggcgagcata tccgctgagg
     1021 attccggacg accgatatcc agaatggcat cccacatcga tttgaatgcc ttccctgttt
     1081 tatccgccca ggtctccagt gttcccatgt tttctttcag gcgacgggtc tgctcatcaa
     1141 agcctttcgt ggcgatatcg ttcgccgcct gcaatgcccc ggcctcgtct ccggaacgct
     1201 gcagctgtgc aacatacgca atctgctctg ccgtcacgtt acggaactgg cgcgccatcg
     1261 ccatcagtcc cgacgtcggg tcagtggtca gcttcccgaa ggcttcagcg actttatcca
     1321 cctccacacc ggatgcagac gcaaaacgcg cgacactctg gttgatggca tcaaactgtt
     1381 caccaccacg cacaccggca ttcaccaggg ctgccagtga ctctctcgcc tggttaaacg
     1441 tcagccctgc tgcctgcccg gctcttgaga gagtcagcat acgatcggca gtcagtccgg
     1501 actgattacc ggaaagaacc agggttttat taaacgctga aagcgtggaa tccccctggt
     1561 accaggcgta cgccagcgca cctgtcgcca ccgccagcga ggtgaccccg accatcggca
     1621 gggtgatcgc accggcaagc cccctgaaca tggggatcat cccgccgaag gagtccttca
     1681 cctgaccgcc ctgttgcagc aggatcagcc agggattctg accaccggca agctgcgtgg
     1741 cgatatccgt aaactgtgcg ggcagggttc gcatggccgc tttatactgc ccgacggaaa
     1801 tcccggcttt ttgtgcagcc agcgcctggc ggctcaggcc ctgttcaaca gcactggcgg
     1861 tttttctggc gtcggtatcc agacctgaaa aatgacgcct tacccggctc atctgctcat
     1921 cgaaacggac cgcatccaga ctaaggtcaa taacaagatc accaaccggc tgggacatat
     1981 ctcacacctc cggaaatccc cgctgaagcc atcattaatg cggcatcatc caccatgaca
     2041 tccgccacat ccgcagacga taaaatatcg cgccctccgt ccccaccgaa ccggacgcct
     2101 ccggcaagtc ctgccgcttt ctgcatcagc attttgtcct catccggcct ctccacctgc
     2161 tcttcctcat gccgggggac aagcagactg aaatcagagg gatgcatatc cggatcgcaa
     2221 aaaaacaggc tgagtacagc gtacgtcagc ccggaaaaat gcatatccag ctgggtatcc
     2281 tgaaaataat gcgtgcggta aaaacggtgc cagtcggcat attcggtgga tgtcatcccg
     2341 gcaagcatgg cgcgccagtc gggtctcccc atctcacgcg ccagtctgag ggcaaagttc
     2401 agctcgccgt cgaagacttt cccgcagaaa aatcatcatc agtcagcgtg ttatttttcg
     2461 ccacttcagt aatatcagta tccggacgaa cagcttcgat catcccggac aggcacaaca
     2521 ccacgtcttc cgcccgggca atggcatcgg caggccaggt ggtgagcact tcctgctcta
     2581 tcttcatcac ggcctcattc attgacggtg actccgtttt ctgtggatgg ttatgccaca
     2641 gggacatcgc caccagaaac gcgccggttc tgacaaggtc ttccacgctc acctgcaggt
     2701 taccgctggc ttcagcctgt tctgcccgtc gtttcagcag ggcaagatgc tcgatacgct
     2761 gcagcgcaga cagctcggaa agcgtgacgg acacaccgtt atattcaaat tgttcggttt
     2821 tcagaaacat gtattacctc cgtttaccct gcagcgcccg cttcagtaac ggtgacttca
     2881 gccactgcgg cgaactgacc atttccgctc accacaggga tctgcacctt acctgtcgcc
     2941 acgccgttta ccgtaattgt catatctttc acactaatgg tggctttcga cggatcggcg
     3001 gaaaccgctc tgaacgtctt gtctgttgca ctttccggct caaaagaaac cgtcagggtg
     3061 gttgttttcc cttttgccac cgtaccggat gtcggcgtca ccttaatcgc actgaccggc
     3121 gtaatttcgc tgcgttcttc cgccacagaa ggtttaccca cgttggtcac tttcaccgta
     3181 cgggtgatca cttccttcgc cgttacggcc ttaccgatac tgctgaccca gccacggaac
     3241 acatccaccg tgccgttcgg gaaacggatt ttataggccc gcacatcccc gctttcaaac
     3301 cagcctataa gccctttctg accttcttct cccggtttcc aggccagcgt aaaactggta
     3361 tctcctgcag acttctgccc ctgcccggtc gcagtccagt ccgcgtcttc atcatccagg
     3421 tagttatcat cgtagggttc tgccgtcatc tcgcccggcg tcagatcctt tacctgtgcc
     3481 agtcgctgcc agttatcgtc tgacaacggg tttgcatagg catcaccctg accgttataa
     3541 acccacaggg tggtaccggc accttttacc ggtgccagag gatttggtgt tggcatatcg
     3601 tcctcacatc tcgtatgtaa tggaataagt caaatcagca gaactccata acgccatatc
     3661 gtcatcacga cgatactcat agccctgcgt aaccatcgtg gtaatcaggc ctgacagtgc
     3721 cgggatcgca ctcatcgccg gataaatccg gctttccatc cacgaatcca gctctgaatc
     3781 cggcacctga gcaggcagga aaacttcaat atgcagctcc gcctgccagg tatcgctgtc
     3841 cagcgcttcg cccgtgtatt cagcgccggt gagataaaca gcaattgccg gaaaatccgc
     3901 ctcatcaaaa acagcggggc gaccatcaaa cagcgtcgcc ccgtgttcat gctgctcgag
     3961 tgcatccagc actgcagcac ggatatcagt atgtttcatc gctttatcac aatccttagt
     4021 tgttgtttca acgcatagcc cagttctttt ggcagacgtt ctcgccggat acggttaacg
     4081 ttctcatcaa aagcctgctt cagtggggcc gccatcggaa ttttaaccac ctgaatggga
     4141 agacgattac gctttttcct tcctttatcg tcattgccct tcgcataccg ggcttctggc
     4201 aaacgttgca taacatgcca gcgcccatta tttaatcgct ggatgaatgc ccgctgataa
     4261 cgatgctgac cggctttaag tatgctgttc ggacgatgac caagcatcct gatccccagc
     4321 ttaatagcag ggagatcacc gcggttaacg ataattctgg cattcggatt tctgaccgtc
     4381 gcccgtttca gtctggaccg ttcctttacc agtttccggc tcaccctggt ctcccgggca
     4441 acctgtgatg aagactgatt aatcgccgtt gtagccacgc ggttaatagc cattgctgaa
     4501 gccgacggaa tggcgttttt acgaacccgg ctcagatttt caatcgcctg atcaagccct
     4561 tttatcgcca taattcaccc tgcgtttatc gtcgccggtt aactgcgggt ggttgcccac
     4621 ggttcagcca gagataacaa ctgcccccgt catccggaga aacacgatcc acccagaacg
     4681 tctcaccatt aatggtcaac gtgtcaccac gccgcacggc acgaaccgta tccgtccgca
     4741 caaataatga cgggctgctt ccttcaatac ggatcccact actggcaaac cccagcgact
     4801 ccggatcgtc aaaaaccccc tgaacttcgc cgccacgctg tgcaccggag gtgaactgcg
     4861 cactgatgcc catcacttca acaatcgtac tgtccacccc ggcaagggcg gcatcaaagg
     4921 cattctgaaa atcacgcata aacagccatt ccaccatcaa cgtgtgtttt tgcatctgag
     4981 gacataatca gaatcacccg accaacatcc gcaagctcaa cggattcccc cgtttcacca
     5041 tcaacgccac agagatggag gcaggtcaga actctgatgc gcgttaacgc gccggatgtt
     5101 tcctcacgaa catcatgagc cgcgttttcc ctctcccgga tatccatatt cataacctgt
     5161 acatcatcgc cggatgactg catttcctct tcccattctg ccacccgctg cgctatctct
     5221 gcggcactcc cggatatatc cggctcacgc cccagaatca gggccagttc atcaagccgt
     5281 ttcagatttt gctctttcgt tgccatatca tccccctgtg aaaaaagaca cgggggcatt
     5341 tcgcccccgc tcacggatta tttcacctgt accaccacaa actcatccgg gtccggcaac
     5401 accatcagcg gcgcggactg cgtcatggta aattcacggg cgggatcccc taccgtcagc
     5461 cagtgtttcg gataacggga agaggccacc acaccttcgg acaacgcctg cgcatcctga
     5521 atggcaccat agcaacgaat gccctctgct gccgtattcc ccaggaccag cgagccctca
     5581 ggcagataac gtttttcggt accgtcctct gccacataag acgtttttgc caccacaatg
     5641 gccagatcgc cgtaataccc tttgaaggac accaccgcgc ccaggtcttt caccgctgtt
     5701 tcgagttgcg aattagagcc gcgacgggta tccagttttt cgcggaacaa tttaaagcca
     5761 ttcagcagac gccagacggt accgtccata atggcaatat tcacaagacc gctggcctgg
     5821 tcgcagtaga ggtcaagatc atgtgtagga tcgaacgtgt cacgatcctg ttttgaccac
     5881 tccttaccac taccctgagt gatgttattc ttcgtcgacc tgccaaaatc gacctcaatt
     5941 ttctcgaact ggtctccttc catcgtgtat ttgccataca gcacggcatt caccgcctgc
     6001 atttcttcca cctggacaat ggcgtgctct tcctgtttga ggttatcagt gatgatacgc
     6061 agacgacggt aggccgggtc gttcagctga gccggatctt caccgggaag acgctcaacc
     6121 gcctgctggt aattaaattc gtgtttcggc ttgacgtagc ccggacgtaa cacgcgggtt
     6181 tcaccaccac gatgacgcag cacttttcct tcaacaaccg gggaaacata ggccgccacc
     6241 ggcgtttttc cggtaatttt gtccagcatc acctcttcgg tatggaaatt caccgtacgg
     6301 cgg
//
LOCUS       DAZGTR010000202         6284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGTR010000202 DAZGTR010000000
VERSION     DAZGTR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.204"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..631
                     /locus_tag="ACHLCY_004649"
     CDS             <1..631
                     /locus_tag="ACHLCY_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFP0371741.1"
                     /translation="LSNIDIIIDLRDELTEDKINSGELYTLAMGLINNLAHLNEYRKV
                     ILSGGSFPTDLSDISVGLYSQPRIEWILWQSLMNRKELARNVIYSDYGVQHPDFNRLS
                     TRFPSVSASVRYSGDNDFWVFRGRVANRFGYEQYGKHSEDILAHREYSGPTFCAGDRD
                     IEYYANEYQAYKANPSGNYKFGSPEVWRRIGQNHHITKVVEQLATLYGL"
     gene            complement(591..1169)
                     /locus_tag="ACHLCY_004650"
     CDS             complement(591..1169)
                     /locus_tag="ACHLCY_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HFP0371742.1"
                     /translation="MRDIDVRKAVHAKILRDHHKDPDTLIIDEFTMNLGASRADIAVI
                     NGLIHGYELKSKSDNLLRLPAQVQHYSSVMDKVTLVVSDCHLYDALSIVPSWWGIKQV
                     TQGARQGIHLKTIRTSKLNPQVDKLSLTMLLWKDELLSLLSDVGELQNLKNKPKRVLW
                     SKLANSMDVGELREAVRVKLKARKEWRVAQQP"
     gene            complement(1475..1786)
                     /locus_tag="ACHLCY_004651"
     CDS             complement(1475..1786)
                     /locus_tag="ACHLCY_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371743.1"
                     /translation="MNEIKEIPVVRDEYGCWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGNGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            complement(1779..2033)
                     /locus_tag="ACHLCY_004652"
     CDS             complement(1779..2033)
                     /locus_tag="ACHLCY_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371744.1"
                     /translation="MSESKCQVNGNKIEPCAALAKSLEHDAEYTMRKGLLIYKIWNES
                     LTRGPDFVMLRSGEFSKLPVRVSFCPFCGESLKTWENRNE"
     gene            complement(2030..2452)
                     /locus_tag="ACHLCY_004653"
     CDS             complement(2030..2452)
                     /locus_tag="ACHLCY_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0371745.1"
                     /translation="MAKVFTQEEQEKIKGQVVELVRQSGRETLRQLETKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRLNIICLECRKSEVMQRVLAFYQGNFEEVVR"
     gene            complement(2493..3455)
                     /locus_tag="ACHLCY_004654"
     CDS             complement(2493..3455)
                     /locus_tag="ACHLCY_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371746.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(3478..3903)
                     /locus_tag="ACHLCY_004655"
     CDS             complement(3478..3903)
                     /locus_tag="ACHLCY_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0371747.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(3900..4202)
                     /locus_tag="ACHLCY_004656"
     CDS             complement(3900..4202)
                     /locus_tag="ACHLCY_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0371748.1"
                     /translation="MTLKEFIKSLRVGDAKKFAARLGVSSSYLSQMASGRTAISPTRA
                     LMIESATEGQVSRAELRPHDWELIWPEYASGIRLGQTHVVHAEGDCSACLSDGVDS"
     gene            4300..4671
                     /locus_tag="ACHLCY_004657"
     CDS             4300..4671
                     /locus_tag="ACHLCY_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371749.1"
                     /translation="MQKKEIRRLRLKEWFKDKTLPPKEKSYLSQLMSGRASFGEKAAR
                     RIEQTYGMPEGYLDAEYAEQPGVSPPHAGLTSNQLELLQIFSAFPEDEQRQIISELKQ
                     KKESMEDLIARWIAAQKCRRA"
     gene            4692..4883
                     /locus_tag="ACHLCY_004658"
     CDS             4692..4883
                     /locus_tag="ACHLCY_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stability determinant"
                     /protein_id="HFP0371750.1"
                     /translation="MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDE
                     VERRMAERFAKMRKERSKQ"
     gene            4885..5163
                     /locus_tag="ACHLCY_004659"
     CDS             4885..5163
                     /locus_tag="ACHLCY_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0371751.1"
                     /translation="MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLS
                     EHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVEVVNVIHARRQFP"
     gene            5516..5817
                     /locus_tag="ACHLCY_004660"
                     /pseudo
     CDS             5516..5817
                     /locus_tag="ACHLCY_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5889..6107
                     /gene="ydfC"
                     /locus_tag="ACHLCY_004661"
     CDS             5889..6107
                     /gene="ydfC"
                     /locus_tag="ACHLCY_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP0371752.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKINAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
BASE COUNT         1705 a         1540 c         1353 g         1686 t
ORIGIN      
        1 tttatcaaat attgatataa ttattgactt aagagatgag ttaaccgagg ataaaattaa
       61 ttccggcgaa ctatatactt tggcaatggg attgataaac aatctggcgc acctcaatga
      121 atacagaaaa gtgattcttt ctgggggttc atttcctaca gatctcagtg atatttctgt
      181 tggtctatat tctcaacctc gaatcgagtg gattttatgg cagagtttaa tgaatagaaa
      241 agaacttgcc agaaatgtga tttatagtga ttatggagta cagcaccccg actttaatag
      301 gctttcgacg cgattcccta gcgtatctgc cagcgtcaga tactctggag ataatgactt
      361 ttgggtattt cgaggaagag tagctaatcg ctttggttat gaacaatatg gtaaacatag
      421 tgaggatatt cttgctcatc gagaatattc agggcctaca ttctgtgcgg gagatagaga
      481 catagaatat tatgcaaacg agtatcaagc ctacaaagcc aatccctctg gtaactataa
      541 atttggtagc ccagaggtat ggcgtaggat tgggcaaaac catcacataa ctaaggttgt
      601 tgagcaactc gccactcttt acgggcttta agtttaactc gaacagcttc acgaagctcg
      661 ccaacatcca tactattggc gagttttgac cataagacgc gtttaggttt atttttcaaa
      721 ttctgtagct cccctacatc acttaatagg gaaagcaatt catctttcca aagaagcatt
      781 gttaaggaaa gtttgtccac ttgtggattc aacttgctag ttcgaattgt ttttaaatgg
      841 ataccttgcc gtgcaccttg cgtaacttgc tttatccccc accatgatgg aactatgctt
      901 aaagcatcat aaagatggca atcagagaca accaaagtta ctttatccat cactgatgag
      961 taatgttgca cctgcgctgg taatctgagc aagttatcac tcttgctctt caactcataa
     1021 ccatgtataa gcccattgat cactgctata tcagctctgc tagcccctag attcatcgta
     1081 aactcatcaa tgattagggt gtcaggatct ttatgatgat ctctcagaat cttggcatgc
     1141 acagcctttc ttacatcaat gtctctcata ccatagttgc gctttcatcg actctctcaa
     1201 gccatgattc tatatcatgg ttcgcatgaa tgccatttga tatgaccagt ggttaaacat
     1261 tgcacagaaa agtggatgtg tattttaaca aaagcaatga cggctaacca tgttggtatg
     1321 tcgataaaat gtttcaacgt aagcattatc tgttccgccc tttcagacgg cctcctgatg
     1381 ttctgagggt gcagaatccc tccggttaag ggtttaataa aaatcgtttc tgatttaaat
     1441 cttcagtatt tagttgttag tcggtttata gcctttatgc ttcggcctta tttctcagcc
     1501 atacacaaac cgggccatct tcggtgtcat gtattgaacc aataaaccat ccattgccct
     1561 ctggtcgttc cggttcccat gcagaaatat cagcatcaca cgcatcaagg tcagcacatc
     1621 cttcatctct gaagcagagg acgtattgaa gattattttc ctccatccag gcgttaaact
     1681 cttccgttga aatatattcc cgaccgtcac agaatttttc atattcagga tgcgtccagc
     1741 agccatattc atcacgtact actggtattt ctttaatttc attcatttct gttctcccac
     1801 gttttcagac tttcaccaca gaacggacaa aatgaaaccc gaactggtaa tttagaaaat
     1861 tcaccggaac gcaacatcac aaaatcagga ccgcgagtta aactctcatt ccagattttg
     1921 tatatcagca gaccttttcg catcgtgtat tcagcatcat gctcaaggga ctttgccagt
     1981 gctgcacatg gttctatctt gttgccatta acctggcatt ttgattcact caccgcacca
     2041 cctcctcaaa attcccctga taaaacgcca gtacgcgctg cataacttcg ctcttccggc
     2101 actcgagaca gattatgttc agacgcctgt cgtagcgacg tatttctccg tcaggtaatg
     2161 accagataag gtccggatca accgcagatg gtttcttcag ctttgccctt gagagctttt
     2221 tacgggcatt ttgccagtcc ttacgcgcct gttcagacgg gaataacccg taaccagagt
     2281 tgtatacatc gccactggca accagctctc tggccagaac gctcatcaga tatcttgttg
     2341 ccccagtttt agtttccagt tgtcgtaacg tctcgcgccc actctggcgt acgagttcaa
     2401 caacctgccc tttaattttc tcctgctctt cttgtgtaaa aacttttgcc acaagccctc
     2461 ctgaaaatta cctcatgacc agaaattaac acttaccccc tgaagcccgg cggaatttca
     2521 gtgtccggtt cagaaatgtg attcacgcaa cgctgcgcag gcgaacgccc caggcggata
     2581 accagttcat cccatttttc ccggagtttt gccggactca tgatgttttt tacccagaac
     2641 gaatcccgct ggagacgccc aaacatttca caaatttgtc tgtgagttct gccatccagc
     2701 atccgcattg tgcgaacgtc attggcccat gctgtccagt tgggttcttt cggtctagtg
     2761 atctcgccat catcgctggc cgcctgctcg taaagactca cgattcgtcc ccagatccac
     2821 tgtgcgcaca ccaaatcttc ctgacttccc cactggcgtt ttttcgcact gaacacaacc
     2881 gcgtcagggt gtcgggttaa aaaatcctgt tcagccgtct gcgggtccgg ttgcgaagcg
     2941 tccggacaag aagatctttt atctgacgga tcaggtttta atactgacgg atcggggtca
     3001 atcatcgccc ccctaatcgg cagtttttta tcaacagttg atccatcaaa atttgacggg
     3061 tcaaccgttg aggggtcaat atttgacggg tcaactgtta acgggtcatt ttttgccggg
     3121 ctaatttttc ttttcggttt atatgactca cgcgccgccg cagcagctgc ttcgagtttt
     3181 tccacattaa gccgatagat attgcttaca ttacgcccac cgaccttacg ctcttccttc
     3241 gtcagccagc cctctttcgc cagttctgca atagccgatt tcactgtgga ttcacttctt
     3301 gcaccgatct gacgccggat agtttcaatg gcaggccatg acacgccctc gtcattgctg
     3361 tagtctgcaa gacgggccat aaccgccacc ctggataaga tcatgccggt gaaggcgcac
     3421 ccttcccaga caagaccatg aagcttgctg ctcataaaac ccccgaacac cgtgctttta
     3481 gtgcatcacc acagcattcc ctgccgggcc gccgcgattc atctggtcat acaaaacaac
     3541 cgctgacgca acaaaatcat cgacatcctt caccagccga tccctccgtt cgacgatctc
     3601 acggtaatat tcagaactgt ggctgcgcat acgggccacc agcaaaggcg gcatcgcctt
     3661 ttcgatcgcc ggtaacagag cctgcatttt ttcaacagca tcaggggtgt ctttatccag
     3721 ccaacggaaa attttctggg tattacgggc cagggcttcc ggatggctgt cgtcatacag
     3781 ttccgggaac gtcattccca gctcgaaata cgctttggta attttcgcag ccggtacttt
     3841 ttcgccgtcc ggatgcgccc agacattcat cgccatgcgg atgtgttcat gcttgatttt
     3901 catgaatcaa ctccatcaga taagcatgca ctacaatcac cttcagcatg aactacatgt
     3961 gtttgcccca aacgaatgcc gctcgcatac tcaggccaaa taagctccca atcatggggt
     4021 cgtagctccg ccctacttac ttggccttcc gtcgcagatt cgatcataag ggcgcgggtt
     4081 ggagatatag ctgttcgtcc agacgccatt tgcgataagt aagatgacga tacaccaagt
     4141 ctggccgcga atttcttagc atcaccaacc cttaatgatt taataaactc ttttaatgtc
     4201 ataccttcct cggtttagtg tttttttgcg agtttagtgt ttaataaacc attaagtcaa
     4261 gtatttgctt gtttagtgat tactaaagat aattaccaca tgcaaaaaaa agaaattcgc
     4321 cgtttacgtc tcaaggagtg gtttaaagat aaaactctgc cacccaaaga gaagagctac
     4381 ctatctcaac taatgagtgg gagagcctcg tttggagaaa aagctgccag aagaatagag
     4441 caaacatacg ggatgccgga agggtatctg gatgcggaat acgcagaaca accgggggtt
     4501 tctccaccac atgcagggtt aacgtctaat caactggaat tattgcagat tttttcagcc
     4561 ttccctgagg atgagcaacg ccagataatc agcgagttaa agcagaaaaa agaatcaatg
     4621 gaagatctca tagcgagatg gattgcggcg caaaaatgcc gccgcgcctg agttataaaa
     4681 ccggaggaaa catgaataga gccctttcac caatggtttc tgaatttgaa accattgaac
     4741 aagaaaacag ttacaacgaa tggctgcgtg cgaaagtagc aacgagcctt gcagatccgc
     4801 gcccagcaat tccccatgac gaagttgagc gcagaatggc agaacgcttt gctaagatgc
     4861 gcaaggaacg gagcaagcag taaaatgtta cccgtgttat ggcttgaaag cgcagatacc
     4921 gacctagatg atataactag ttatattgct cgtttcgaca tagatgcggc tgaacgctta
     4981 tggcagcgat taaggggttg tgtgctgccg ttatccgaac atccgtattt atacccacca
     5041 agcgacagag tacctggctt gcgtgagatt gtagcccacc ctaactatat aattctatac
     5101 cgcgtaacaa catcaagcgt tgaagtagta aacgtgatcc acgcaagacg ccagtttccc
     5161 taactttcac taccaataga aacataacaa ccgcaacgac tttatcaaaa gcgttgtgtt
     5221 tgttatgtcc cgcggtttag tttttacttg acttaagttt aatgtttatt aaactaaaaa
     5281 taccaaccca ccccgcccca cagaacgcag ggaaatactt cgagttaccc ggcagtggtc
     5341 aggggttaag tatccagccc gaggcgtatg aacatgacgg cagggttcaa ctttaataac
     5401 tatgcagcag gtttttgttc cgctaccccg gcgttaaggg gaaacagagg gtttctcagt
     5461 gggcgaagtc aaacatcaga atggaaggca tcccgggatc ggcaaagaag cagcaatggc
     5521 gctttatatt gacatcagcg ccattgcagg acaggtaaga gttatcagag cggtaactaa
     5581 gcggtatgcg cctttacttc agaaagtctc tggtgagtgc accgaagata ttgtcaacga
     5641 tttcgtcatc gaactgcgag gactcatctt cagtttacaa ggtgaaccac aatttttgca
     5701 gatggctccc gcgaaactgt cagagccctg cggcttaaag gatgtgtcaa agacttcgcc
     5761 accacattct gggcaagaaa acttgattgt attcataacc aatttcctct cgagtaacag
     5821 acccctcaga ggataccacc tcgcctgacg tggttaaaag caggcaacgc taaccacaag
     5881 gagccgacat gcagaaacga gaacccgtca tcatcgcgcc agactatacc gatgatgaac
     5941 tttatgagtg gatgcaccag aaaattaatg cagcgcagga tctgaaatgg gccaatgaag
     6001 ccagggctaa gcaggctgaa aatctgtccg ctctggagca ggatatcacc aatctggaaa
     6061 aagcagcggc attaagcatt gccagaatga ttacataccc gcgttaatag ctaaccaacg
     6121 aagctaaggt tggtaattaa ggagttctcc acgggtgagg tggagtgcgt gcgccggaca
     6181 cgggtgagca tacggcactg acagtttact gaaaggatat ttccctgaaa agtcagacca
     6241 taacgcgaaa tggcgcggcg gtaagtatgg aggggttatt cctt
//
LOCUS       DAZGTR010000203         6273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGTR010000203 DAZGTR010000000
VERSION     DAZGTR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.205"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            42..971
                     /gene="pfkB"
                     /locus_tag="ACHLCY_004662"
     CDS             42..971
                     /gene="pfkB"
                     /locus_tag="ACHLCY_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP0371753.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALDVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1072..1362
                     /gene="ghoS"
                     /locus_tag="ACHLCY_004663"
     CDS             1072..1362
                     /gene="ghoS"
                     /locus_tag="ACHLCY_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0371754.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1468..2328
                     /gene="yniA"
                     /locus_tag="ACHLCY_004664"
     CDS             1468..2328
                     /gene="yniA"
                     /locus_tag="ACHLCY_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP0371755.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2369..2905)
                     /gene="yniB"
                     /locus_tag="ACHLCY_004665"
     CDS             complement(2369..2905)
                     /gene="yniB"
                     /locus_tag="ACHLCY_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP0371756.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3052..3720
                     /gene="hxpB"
                     /locus_tag="ACHLCY_004666"
     CDS             3052..3720
                     /gene="hxpB"
                     /locus_tag="ACHLCY_004666"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP0371757.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3883..4473
                     /gene="ydjM"
                     /locus_tag="ACHLCY_004667"
     CDS             3883..4473
                     /gene="ydjM"
                     /locus_tag="ACHLCY_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0371758.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4606..5997
                     /gene="tcyP"
                     /locus_tag="ACHLCY_004668"
     CDS             4606..5997
                     /gene="tcyP"
                     /locus_tag="ACHLCY_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP0371759.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6001..>6273)
                     /gene="ydjO"
                     /locus_tag="ACHLCY_004669"
     CDS             complement(6001..>6273)
                     /gene="ydjO"
                     /locus_tag="ACHLCY_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFP0371760.1"
                     /translation="LLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQAHS
                     LSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1542 a         1549 c         1575 g         1607 t
ORIGIN      
        1 tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt atctatacgt
       61 tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat cccgaaggaa
      121 aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac gtcgcccgcg
      181 ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc gcgaccggcg
      241 aacacctggt ttctctgttg gcggatgaaa atgtccccgt cgctactgta gaagccaaag
      301 actggacccg gcagaattta cacgtacatg tggaagcaag cggtgagcag tatcgttttg
      361 ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag caagttctgg
      421 aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt gtgaagctgg
      481 aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc atcatcgaca
      541 gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg gttaagccta
      601 accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac gatgtccgca
      661 aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt tcccttggtc
      721 cacaaggagc gctggatgtt gatagtgaaa actgcattca ggtggtgcca ccaccggtga
      781 aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca ctgaaactgg
      841 cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg agtgcagcca
      901 cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt tacgcttacc
      961 tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac ctgtcggcaa
     1021 cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac tatggccagt
     1081 ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt gactgaaatt
     1141 aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac ggatgatgag
     1201 ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca aagtgaagaa
     1261 gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga tcctgaaatc
     1321 accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt tgggcaataa
     1381 tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct aaccttatga
     1441 tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct tttgagcgag
     1501 cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga agtccatgcc
     1561 gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga aagggaactg
     1621 cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag taaaaccgtc
     1681 accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct ggtgatggat
     1741 tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca gcaaattgcg
     1801 cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa ctcgctctcc
     1861 acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc tgaacaacgg
     1921 attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa tatcgacgcc
     1981 atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc tctgttgcac
     2041 ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat tttcgaccct
     2101 gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca tactgaacag
     2161 ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga tttccttgaa
     2221 cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt tggcggtcag
     2281 catttggtta ttgctcagca gtcattggat agattattag cagcatgata tgggttgagg
     2341 ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa aagaacacat
     2401 aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc aggaaaatag
     2461 tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc tcttcccctt
     2521 tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc gcctggcgac
     2581 tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg aaaatcaccc
     2641 agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac cagaacagat
     2701 tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag tcgagcatga
     2761 ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc aaagaaatca
     2821 atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc aaaacagcaa
     2881 tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc gtttcactta
     2941 gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata ggcgcttaat
     3001 cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt aatgtcaacc
     3061 ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga ctcagaacct
     3121 ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat ctcccgtcgt
     3181 aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct ttggtacgcc
     3241 cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat tgcccgtgcc
     3301 atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc cgttgcgtta
     3361 tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca tatgctggaa
     3421 aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc ggctgaaaaa
     3481 ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa actgggcgtt
     3541 gacccactga cctgcgtagc gctggaagat tcagtaaatg gcatgatcgc ctctaaagca
     3601 gcccgcatgc gttccattgt cgttcctgcg ccagaagcgc aaaatgatcc acgttttgta
     3661 ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct tctcggttaa
     3721 tgaccagggg cagtgatctc gctgcccctg gttctttatc tgaatttgca ttcaactgac
     3781 ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg cactgtataa
     3841 aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc ggaaggtcac
     3901 cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac gcccgtgctg
     3961 gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt gttaccggac
     4021 atcgatcacc caaagtcgtt tcttgggcag cgattaaagt ggatatcaaa accgatcgcc
     4081 cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc gctgctggca
     4141 accttttacc ttaaggttcc tgaaagctgg ttcattccgg ctgatgcgct acaaggaatg
     4201 gtgctgggtt atttaagcca catacttgcc gatatgctga cacccgccgg tgttcccctg
     4261 ctctggccat gtcgctggcg tttccgcttg cctatcctgg ttccccaaaa gggcaaccaa
     4321 ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc ccattcatta
     4381 cccgaaaaca gcgcggttcg ttggtcatcg caaatgatca ataccttgca gatccagttt
     4441 catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct ttctggaata
     4501 agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc gccaacacta
     4561 tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa ctttccatta
     4621 attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac ccgccacaaa
     4681 cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt ttttggcctt
     4741 gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt acagtggttt
     4801 aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc gttagtcttc
     4861 gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg caaaatcagt
     4921 tttctgacca tcggtacgct tttgtttacc acactgattg cggcgctggt cggtgtgctg
     4981 gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc agaaactgca
     5041 cgtctgaacg ccatcgaaag taactatgtt ggtaaagtct ctgacctgag cgttccgcag
     5101 ctggtcttgt cctttatccc gaaaaacccg tttgccgatc tgaccggagc caatccgacg
     5161 tcaattatca gcgtggtaat ttttgccgca ttcctcggtg tagctgcgct gaaactgctg
     5221 aaggatgatg cgccgaaagg tgaacgcgtc ttaaccgcta tcgataccct gcaaagctgg
     5281 gtgatgaaac tggttcgcct ggtcatgcag ttgacccctt acggcgttct ggcactaatg
     5341 accaaagtgg ttgcaggttc taacctgcaa gacatcatca aactgggaag tttcgttgtc
     5401 gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct gggcattaat
     5461 ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt tgcctttacc
     5521 agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg tcgtctgggc
     5581 gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg tcagaacggc
     5641 tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt tggcattaac
     5701 ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag ttccgcaggc
     5761 gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc tgcgatgggc
     5821 ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga catgggccgt
     5881 acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca gtggctgaag
     5941 caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca ccgttaattc
     6001 ttatgctggc aacggtccgt tttgtatagg ggccgttgct ttacttttta aatgttccat
     6061 cgcggcgatg catactgact catatttaaa cagatcgtca gcgaaaaaaa tgtccaactt
     6121 atcaatacat tcctggctca atgaatgtgc ctgtagtgag aagcttaaaa cctcataccc
     6181 gagacgtttt ctaagtgaac tatctggaat actcataata tcttcaagca caccacttcc
     6241 cattcccgga agatttagtg gattaaaaag aag
//
LOCUS       DAZGTR010000204         6211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGTR010000204 DAZGTR010000000
VERSION     DAZGTR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.206"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            129..635
                     /locus_tag="ACHLCY_004670"
     CDS             129..635
                     /locus_tag="ACHLCY_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0371761.1"
                     /translation="MAFLLNKSDMASSIGISVQAFDKWGVPPVERRGREVFYDVKTVL
                     EIDRERRQHNQRIPDDEGDLEERLLRARAELTEEQAVAQKLKNQVTEGKLIDAGFCIF
                     ALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLLD
                     EYIRATTE"
     gene            607..2535
                     /locus_tag="ACHLCY_004671"
     CDS             607..2535
                     /locus_tag="ACHLCY_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0371762.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2519..2725
                     /locus_tag="ACHLCY_004672"
     CDS             2519..2725
                     /locus_tag="ACHLCY_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP0371763.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2722..4314
                     /locus_tag="ACHLCY_004673"
     CDS             2722..4314
                     /locus_tag="ACHLCY_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0371764.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQEQKDNPLINILEKFSSWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4304..5809
                     /locus_tag="ACHLCY_004674"
     CDS             4304..5809
                     /locus_tag="ACHLCY_004674"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0371765.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRVDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSAIKAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSIEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGSSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVRGGVSQTSADGDADVKMLMAMP"
     gene            5846..6193
                     /locus_tag="ACHLCY_004675"
     CDS             5846..6193
                     /locus_tag="ACHLCY_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0371766.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
BASE COUNT         1447 a         1491 c         1917 g         1356 t
ORIGIN      
        1 aaaataaata caacaacctt acgatgtgtt ttgatgtcgt caatgcgaaa aatgtcaatg
       61 atatcaaatg gttttgtaaa aacacatggt tgttgtatcg ctttttatcg atggcttatg
      121 gagaggagat ggccttttta ttgaataaaa gtgatatggc ctcctccatc ggtatatctg
      181 ttcaggcatt tgataaatgg ggcgttcctc ctgttgagcg tcgggggagg gaagtttttt
      241 atgacgttaa aactgtactg gagatagatc gcgagcggcg acaacacaat cagagaatac
      301 ctgatgacga gggcgatctg gaggaaaggc tgcttcgggc cagagctgaa ctgacagaag
      361 aacaggccgt agctcaaaaa cttaaaaatc aggtaaccga aggtaagctt attgacgccg
      421 gattctgtat ttttgccctc agtaagctgg caatggcgtt atccagtacg cttgattcca
      481 tccctttatc catgcagcga cagtttcctg atttaacacc gcgccatctt gaccatctga
      541 aaacccttat tgctaagggg gcaaatcagt gtgcgcgggc aggggataaa ttaccggatt
      601 tacttgatga atatatcaga gcaacaactg aataatatga tgagcgctgt cacaactgca
      661 ttacagcccc tgataagggc attgccggtg acgccagttg aatgggctga tcaaaattat
      721 tatctgccta aagaatcttc atatggtgag ggcgaatgga aaacgctgcc attccagatc
      781 gccatcatga acagcatggg gaatgatcag atccgcactg ttaatctgat taaatctgcc
      841 cgtgttggct atacaaagat gttgctgggg gtggtcgggt attttattga gcataaatcc
      901 cgaaacagtc tgctttttca gcccacggat tctgccgctg aagattttat gaagtctcac
      961 gtggaggcga cgattcggaa cgtgccatgc ctgaaagacc tttccccatg gctgggtcgt
     1021 aaacatcgtg acaatactct cacgctgaaa cgcttttcat cgggcgtcgg tttctggtgc
     1081 ctgggcggcg ctgccgccaa aaactaccgt gaaaaatccg tggacgtggt ctgctatgac
     1141 gaactttcct cgttcgagcc ggatgtcgaa aaagagggct cgccaaccct gctgggggat
     1201 aagcgtattg aggggtcggt gtggccaaaa tccattcgcg gctcgacgcc taaaatcaaa
     1261 ggcacctgcc agatcgaaaa agccgctaac gagtcggcgc attttatgcg tttttatgtg
     1321 ccctgcccgc actgtgggga ggagcagtat ctgaaatttg gcgatgagtc cacgcctttt
     1381 gggcttaaat gggagaagga cagccctgaa agtgttttct acctctgtga acatcatggc
     1441 tgcgtgatcc atcagtctga actggaccag agcaacgggc ggtggatctg tgaaaacaca
     1501 ggcatgtgga cccgtgacgg tctgacgttt ttcagcgccc ggggtgatga aattccgccg
     1561 ccgcgctcca tcacgttcca tatctggacg gcgtacagtc cgttcaccac ctgggtacag
     1621 attgtctatg actggctgga tgcactgaaa gatcccaacg gcctgaaaac ctttgtgaac
     1681 accacgctgg gcgagacctg ggaagaggcc gtgggcgaaa aactcgatca ccaggtactg
     1741 atggataagg tggtgcgtta cacggcggcg gtgcctgccc gggtggttta tctgacggcg
     1801 ggcattgact cgcagcgaaa ccgttttgag atgtatgtct ggggatgggc tccgggagag
     1861 gaagccttcc tggtggataa aatcatcatt atggggcgtc ctgatgagga agagacgctg
     1921 ttacgtgtgg atgcggcgat caacaaaaaa taccgccatg cggatggcac cgaaatgact
     1981 atttcccgtg tctgctggga caccgggggg atcgatggtg aaattgttta tcagagatca
     2041 aaaaaacacg gtgttttccg ggtgctgccg gtaaaaggcg catctgtcta tggcaagccg
     2101 gtgatcacca tgccaaaaac ccgcaatcag cggggcgtgt atctgtgtga agtgggaacg
     2161 gacaccgcaa aagaaattct ctatgcccgt atgaaagccg atcccacgcc tgcggatgaa
     2221 gccacgtcgt atgccatccg ttttcctgat gatccggaga ttttttcgca gacagaggcg
     2281 cagcaactgg tcgcggaaga gcttgtggag aagtgggaaa aaggaaagat gcgtctgctg
     2341 tgggataaca aaaagcggcg taacgaagcg ctggactgcc tggtgtatgc ctacgcggca
     2401 ttacgtgtgt ccgtgcaacg ctggcagctt gatctggctg tactggcaaa atcccgggaa
     2461 gaagagacga cccggccaac ccttaaagaa ctggcagcga agctgtccgg aggagtgaat
     2521 ggttacagtc gctgaactgc aggcgctgcg tcaggcgcgc cttgatttat taaccggtaa
     2581 acgggtggtg tctgtccaga aagatggtcg cagaattgaa tatacggcgg cttctctgga
     2641 tgagcttaac cgggcgatca atgatgcgga gtcggtactg gggacaaccc ggcgtcgccg
     2701 tcgtccgctg ggagtgaggt tatgaaacga acgcctgtcc tgattgatgt gaacggcgtt
     2761 ccgcttcgtg agagtctcag ctacaacggg ggcggtgcag gatttggcgg gcaaatggcg
     2821 gagtggttgc caccggcgca gagtgccgat gcggccctgc tacccgcgtt gcgtctgggg
     2881 aatgcccggg cagatgatct ggtgcgcaat aacggaatag cggctaatgc ggtggctctg
     2941 cataaggatc acattgtcgg gcatatgttt ctgatcagct accgtccgaa ctggcgctgg
     3001 ctggggatgc gggagaccgc agcaaaaagc tttgtcgatg aggtggaggc ggcctggtcg
     3061 gaatacgccg aagggatgtc tggcgagatc gacgtggaag gaaaacgcac gttcacggaa
     3121 tttatccgtg aaggtgtggg cgttcatgcg tttaacggcg aaatctttgt gcagccggtc
     3181 tgggatacgg aaaccacgca gttattccgt acgcgtttta aagccgtgag tccgaaacgg
     3241 gtggacacgc ctggacacgg tatggggaac cgttttctgc gggccggtgt ggaggtcgat
     3301 cgatatggcc gtgccgtcgc gtaccatatt tgtgaggatg attttccgtt ctctggtagt
     3361 ggacgatggg aacggatccc gcgtgaactt cccaccgggc gtccggctat gctgcatatt
     3421 ttcgagccgg tggaggacgg gcagacccgt ggggctaatc agttttacag cgtcatggaa
     3481 cggctgaaga tgctcgattc cctgcaggca acacagcttc agtcggccat agtgaaggcg
     3541 atgtatgcag cgacgattga aagtgaactt gataccgaaa aggcctttga atatatcgcc
     3601 ggcgcgccac aggagcagaa ggataatccg cttattaata ttctggagaa gttctccagc
     3661 tggtatgaca cgaatcacgt gacgctgggc ggtgtcaaaa ttccgcacct tttccctggt
     3721 gatgatctga aactgcagac agcgcaggat tcagacaatg gattttcggc gcttgaacag
     3781 gcgctgctgc ggtatatcgc cgccggtctt ggcgtttcct acgaacagtt gtcccgtgat
     3841 tactcgaagg tcagttactc aagtgcccgc gcctccgcca atgagtcgtg gcgctatttt
     3901 atggggcggc gaaaatttat tgcggcccga ctggccacgc agatgttttc ctgctggctg
     3961 gaagaggcac ttcttcgggg gattattcgt ccgccacggg cacgttttga tttttatcag
     4021 gcgcgatcag cctggtcacg ggcagagtgg attggtgccg gaagaatggc cattgacggg
     4081 ctcaaggaag tccaggaatc agtgatgcgc attgaggccg gactgagcac gtatgagaaa
     4141 gagctggcgc tgatgggcga ggattatcag gacattttcc gccagcaggt cagggaatct
     4201 gcagagcggg aaaaagccgg actctcacgc ccggtgtgga tagcgcaggc gtatcagcag
     4261 cagatagcgg agagtcgcag gccggaagag gagacaacac cacgtgagac gtaatctttc
     4321 acacattatt gccgcagcat tcaatgaacc gctgcttctg gagcccgcct atgcgcgggt
     4381 tttcttttgc gcgctcgggc gcgagatggg ggcagcaagt ctttcggtac cacaacaaca
     4441 ggtacagctt gatgctcccg gaatgctggc tgaaacggac gagtacatgg ccggaggtaa
     4501 acgaccggcc cgtgtttacc gggtggtgaa cggtattgct gtactgccgg tgaccggcac
     4561 gctggtgcac cggctgggcg gtatgcggcc attttccgga atgacaggct atgacggcat
     4621 tgtcgcctgt cttcagcagg caatggcaga tagccaggtg cggggcgtac tgctggacat
     4681 tgacagtccg ggcgggcagg ccgccggcgc gtttgactgc gctgacatga tttaccgcct
     4741 ccgtcagcag aagccggtct gggcactgtg caatgacacg gcctgttctg cagccatgct
     4801 gctggcgtcg gcctgctccc gacggctggt tacccagaca tcccgtatcg gctccattgg
     4861 cgtgatgatg agccatgtca gctatgccgg tcatctggcg caggccgggg tggatatcac
     4921 gctgatttat gccggggcgc acaaggtgga tggcaatcag tttgaagcct taccggcaga
     4981 ggtgcgtcag gacatgcagc agcgggttga tgcggcgcac cggatgtttg ccgaaaaagt
     5041 ggcgatgtat acggggctgt ctgtggaagc tgtcacgggg acagaggctg ccgtttttga
     5101 aggtcagtcc gctattaagg ccggactggc ggatgaatta atcaatgcgt cggatgccat
     5161 cagcgtgatg gctgcggcgc tgaacactca tgatacagga ggcactatgc cgcaattaac
     5221 tgcaacggaa gctgccgcgc aggagaacca gcgagtgatg gggatcctga cgtgtcagga
     5281 agcgaaagga cgtgaacagc ttgccacgat gctggcagga caacagggca tgagcattga
     5341 acaggcccgg gctattctgg ccgcggcggc accgcagcag ccggtggcat ccgcgcagag
     5401 tgaagccgat cgcattatgg cgtgtgaaga agcgaaaggt cgtgaacaac tggcggcaac
     5461 gctggcggcg atgccggaga tgacggtgga aaaagcccgc ccgatcctgg ctgcctcacc
     5521 gcaggcgaat gccgggtcct cacttcgtga tcagatcatg gccctggatg aggcaaaagg
     5581 ggcagaagcg caggctgaaa aactggcggc ctgcccggga atgaccgtgg agaacgcccg
     5641 ggctgtgctg gctgcgggat caggtaaggc cgaaccggtc tctgcatcca caaccgccct
     5701 gtttgaacat ttcatggcga atcattcacc ggcagcggtg cggggtggcg tgtcacagac
     5761 gtcagcagac ggtgatgcgg acgtgaaaat gctcatggcc atgccatgaa gtcagtgctg
     5821 accatcaata tgaggtttta acaaaatggt gacgaaaacc atcactgaac agcgtgcgga
     5881 agtacgtatt tttgctggta atgatccggc tcataccgcc acaggcagca gcgggatttc
     5941 ttctgcaaca ccggctctga cgcccctgat gctggatgaa gccaccggga aactggtggt
     6001 ctgggatgga cagaaagccg gtagtgcggt tggcatactg gtactgccgc ttgaaggcac
     6061 agagacggtg ctgacctatt acaagtcggg gacctttgcg acggaggcaa tccgctggcc
     6121 tgacagtgtg gatgaacaca aaaaggcaaa tgcctttgcc ggcacagccc tgagtcacgc
     6181 ggctctgccg taacacgtta tcaggccacc a
//
LOCUS       DAZGTR010000205         6174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGTR010000205 DAZGTR010000000
VERSION     DAZGTR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.207"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1321)
                     /locus_tag="ACHLCY_004676"
     CDS             complement(<1..1321)
                     /locus_tag="ACHLCY_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP0371767.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQISVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSLQLS
                     YYEKNIREKQISLNEYEEAFKKSDFKILLGRLTSWSMLYLKQHLRRNV"
     gene            complement(1562..2608)
                     /locus_tag="ACHLCY_004677"
     CDS             complement(1562..2608)
                     /locus_tag="ACHLCY_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP0371768.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(2712..2861)
                     /locus_tag="ACHLCY_004678"
     CDS             complement(2712..2861)
                     /locus_tag="ACHLCY_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371769.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            3283..3474
                     /locus_tag="ACHLCY_004679"
     CDS             3283..3474
                     /locus_tag="ACHLCY_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0371770.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(3527..3760)
                     /locus_tag="ACHLCY_004680"
     CDS             complement(3527..3760)
                     /locus_tag="ACHLCY_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371771.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(3856..4479)
                     /locus_tag="ACHLCY_004681"
     CDS             complement(3856..4479)
                     /locus_tag="ACHLCY_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0371772.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKVGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(4568..5077)
                     /locus_tag="ACHLCY_004682"
     CDS             complement(4568..5077)
                     /locus_tag="ACHLCY_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371773.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            5535..5993
                     /locus_tag="ACHLCY_004683"
     CDS             5535..5993
                     /locus_tag="ACHLCY_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371774.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
     gene            6093..6174
                     /locus_tag="ACHLCY_004684"
                     /pseudo
     CDS             6093..6174
                     /locus_tag="ACHLCY_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
BASE COUNT         1800 a         1473 c         1233 g         1668 t
ORIGIN      
        1 aaacatttct tcgcagatgt tgctttaaat aaagcataga ccatgatgtc aggcgcccca
       61 gtaaaatttt aaaatcagat tttttaaatg cttcctcata ttcattcaga gaaatttgtt
      121 tttcccggat atttttttca taataagaaa gctgaagaga agtaaatagc gccagccttc
      181 tctccggaat catcaatggt ttgactttca gtatcatgaa gtttaacaac aaatcaattc
      241 tgtttctgaa tccaatccgc ctgtcagaaa ggtaatgaat ataagccatc aggtcggttg
      301 ttttttgtga ggaaaatcgg tatttgtgag aaaaaagctc ctccggtcga agattctgac
      361 tctgccattg ctgcaggtat ttatactcaa tattcagctc attaatttct gcttttaacg
      421 aagcgacctg gttaatggca ttcaggtgtt ttttcacttg ttgaagtaca tcatcaatcg
      481 catttgcaga cggtgagtca ggtaagactt cctcagaacg cgaaatcgac gttgaaaaaa
      541 actgttgcct gttctccgta ctccccagtc gtgccatcac atatcccagt tgctgtttgt
      601 ccatcttttc ataaacatta ctgacagctg agttattatt ggacaaaatt gccactgttt
      661 tattctgaat taatatatta gcaacaatat tgagtatagt ctgtgtttta cctgttccag
      721 gtggtccttc aataactgaa atctgtgagg aaaatgcccg ttcgacagcc agcaactgac
      781 tttcattaag accaaacgga aaaatcaaac cccatggcga atcacgtttt ttactctgtc
      841 ctgaacagta tgcatgcaag gcggtatccg gatgaggaac tattttcttt atctgcctaa
      901 ggatattctc atcgatattt ctgttattac cacgggcata aaaaatacgt tcattagcca
      961 ccctacacaa gtaattaaac acggcatgct ctgtaaatgc tgaacactgt tgaaagtcaa
     1021 ggccggtcgt tttcatgatg tagggtcgtt caccctcgcg gtaataaaca gcagcatatt
     1081 tatcaccata tataataacc agttcagccc gggatgttac agcgttccct ttcctttcga
     1141 ggaaaacatt tcgcaaattt acagttggcg ttatctgaca tacagaaagc ggacgagtat
     1201 attttctccc gctgcgaaaa tgacaggtca gcataacctc tctgtcgtca tttccagccc
     1261 acagcgtcca gtcggtaatt ttatgagtct tattttcacc gtcaacacaa atcgtgacca
     1321 tattcttact cagaaattat catttaactt acggaaaatt gttaaggcac tcccgatatt
     1381 agctccggag gattttccac aactccggga ctgttaacat atttctgcct gccatatata
     1441 cattgaattt agtcataaca gtcaatacca gaattacagg aatgcgatgc cgatacgctg
     1501 gttattgaat actggtagcc cctgagcact aattcatcac gatactctgt atttactgtt
     1561 ttcactgctt tttacaacca ttcccccagg taaaatgctg ctcctccggt gatggctgtg
     1621 tcagaaactg ccatcccgga atagccacgc tgaccgccag caatgccgca cagacaccag
     1681 ccacaaacgg tttcagaaac gtgggcgggg atttctgaag ggagggtgac tgctccgcct
     1741 gcactttccc cttgcgatta tcaaggctca gtgcatccag acgcaccgcg ccatttgccg
     1801 caagcagccc ttcccggtta tccacgcttc ccggcagact cagcgaaaaa tcccctgttc
     1861 ccgtctgaat cagactgcct ttacgatttg acaggctttg tgccgatatc tgcagtgtgt
     1921 cggcatttat cgtaccgcca ttactgctga actgcccggc agtccgggcg gaaagcgtat
     1981 cggcactcag ttgcgcattc tgtgtagaca gatttccact ggttgcattc agctcagtat
     2041 gttgtccctg tatccggctg ttacctaaat ccagcccctg ctcaatgtcc cgtaaatcca
     2101 gcagagaacc ttcgtgtgcc gggaatgagg aatacaactg aatatagtca tcactgtccg
     2161 gtgtcagacg cacatgtcgc acaacaccgg gaataataac cagccggagg ataccgcttt
     2221 ttaaatcctg cgaaggtgcc agaacacggg tggtgacata accatgatcg accagacggt
     2281 tctgcaatgt actcatcagc agattaattc ctttcgcccc caggcaatgc ccgaccgccc
     2341 cgttggcgat tttttgtaaa ggcagccagt gtggtaacgc atcagccccc ttcagttccg
     2401 tctgtttaat ctgaaaacag ggcgtttcaa caggaaaatt aatcttatgg gcaaaagagc
     2461 caggtgcaga aaggcgaaca tcaggggctg aaggcgtcag ttgttgctca agggcttcct
     2521 gacgctgctg ctgaatgata aatgcttcat ctccgctacg ccggacatcc gctgcctgaa
     2581 gagataaagg cgataataaa gcagacatca gtgtcagtga cagcaaggac ggacagcgcc
     2641 ggaataagct ttgttcctca gtcctctcgt tcctgcaaca taattttcca gtcaaacgaa
     2701 aaacctttta ttcatgtcat accaacaatc accagcctcc tgacgatacc tcccaccatg
     2761 ttttgcgatg gcacgctacc gcccttccgg tatttacccg catcaaccgg taccagaatg
     2821 ctgagtaaca aaccagctag aattgactta attttgatca ttatagaata atgatctgaa
     2881 ctacagcaac atgacagatt tatcgatcga tataacacaa atgaacagtt ctttattctg
     2941 acaaatatcg caaaaacagc acattacaaa atttataacc ataattactg gaaaagtgca
     3001 gaaaattatt ccactgaatg cttttaatac agttggttag cttaaaaaat agcaattttt
     3061 gatttcagtc attgaaagtt gcaatgtttg acgattcaac agcatttaag tgagtttcag
     3121 gatatgggaa ggacatgaaa ggaggagcgc aataaaatac acaggaagaa ggggaaaatg
     3181 tttcgtaaaa acagaaaatt aacagcgcac atcaggaaac gcagtctcct gattcagtgc
     3241 taccacatga aaatttagtt aactgttacc gtactaaaat ttatgtcaga agatgttcca
     3301 ttaccgaaag tcaatcagcg atacaaagac gatcatggtg cgcttgtcac cctgaccagt
     3361 gttgaggaaa cccgggtggt gtttatgcag gacggctatc cgcatccctg catgcgaccg
     3421 atgtacaact ttctgggaaa attcaaaccc gaaccccggg aagaaacaga ataaaacgaa
     3481 atccctgccg gcaatcacac tacctgatag cagcataatc cacttattaa ttctcatccc
     3541 cggagacaag cccagaaaac cactccgggt gttcactgta tgccgtgttc agcgcatccc
     3601 tggaaacgtt tatataaaag ctgtcctgca atttgcgggt aacaacttct gccaggcgat
     3661 tccggttatc caccagtccg gcataacctc tctctgccag ccactcattc agttcatgtt
     3721 ccagagacag attgataata tcataatcgg accttgccat tcccgtttcc ttagcacatg
     3781 ttttcaggca cggggataaa gtataaagaa tgtcatactg ctgctgccag agcaatattc
     3841 cccctgatgg ttgtatcaga gatggtatgc cggtactttg cgggttgttc ccgtcaggtt
     3901 atcgtgcacc tccacggtcg tggtcaccac cggcattccg acctttctca gcctcagaac
     3961 atcagccgca atacgctgac tgccaaacca gaacaggccg tccagtgcag tcaccagtaa
     4021 acccgcctcc agtgcatact tcagccgttc acggggcgct ttcacttccc gggcaacctg
     4081 ctgatatggc aatgatgtgt tttcattccc aaccacgcga cgaatacggt gagacagatg
     4141 ataccggtat gtatccggca caccagaaag gctggccttc aggctgtaca cgcagccaaa
     4201 ccgtttatcg ttaaacacca catttttctg gctgatgccc cattcttcac gcagcgcggc
     4261 aatcaattgc ggtgtacggg taagcagcaa gcgaaaaggc agttcaaaac tggtgacgta
     4321 atccacattc agcagtgcaa tacgcaaccg ttcttctggc ccggcctctg tctgccggca
     4381 ctcctccaga acatcctgcc actgccggcg aagacggggg gattcgttcc gttctgtaaa
     4441 acagtattta tccgccagat agtcaattcg tgtacgcata ctgaaaggaa ttccgtataa
     4501 agattcagca gacaaaactt tatcagccag taaaaattaa cggaagcatt gatattcctc
     4561 ccgacaatca ccggatgatt gttgcaataa cctgcgacat aaaggactga acagccgttt
     4621 ttaacgccac gtattgccct gatgaaccag gccgggccac ccgaaaacat ctttccaccc
     4681 ggcattgtcc gccagcactt atcaccgacc aggtctgttg cagtaaatcc accagttgcg
     4741 cacattcttc atcagccatg acgccgactc gcggatggca aatactcgtc tggctgagca
     4801 gcaacagaca tcgtatctgt gctccttcat gttgttcccg caaccagcgt aataccaccg
     4861 gcgctgatgg gctgaccgtg tgattatgct ccgtaatacg ttcgtgtacc cgtcggtgaa
     4921 aggaactaca aaatgtctga atctgttgac catagattcc ttctgccgaa tagagcgtga
     4981 agtgaaatgc caacagattt ggccagtggt ccaccgcctg ctgaacaaaa cgccggattt
     5041 cccccggctc tgaaattaag gcttcggtta tttgcacgaa atgttccttt tcaacccctg
     5101 ttgaattttg tcaagccggt gcagacacag taaagcgcgc atcaacacct gaaaggtacg
     5161 atacaaacac aagttacaaa caataagaac ggaaggacaa ttaatcagtg aggaataata
     5221 actaaattaa ccaaaagaat gttactgaca atactaccaa tacttaaata gcaatatatt
     5281 aattaatgta agaacagatg cccggtagga catatacaca atacatccac aaacacactc
     5341 ttttcattaa atcaatcacc tgattaaacc atgaaagtgt gatgtaagtc aaaattactg
     5401 atactatcaa ttgataatga ttatctctat cattatcgtg aaattcgaga atgaaaggag
     5461 aagtagtcat tataagcctt ttataaatcg tcatcatcat agtaaagtag aacacttttt
     5521 atatgagtta ctatatgaaa atatactgga caagaaaacg tattcctgaa ctttcagcac
     5581 ttccaccttc attaagaaag aaaaatttta ctgacgcata taatgcagca tcttctcata
     5641 tcgaatactg gataggtgct ggcgtctcat ttatctcgat gatgatttta tccagagtat
     5701 atgactttct tttacccgcc caagatactt tcccgggtga tatcatcaga agtttatgtg
     5761 ttgtgtgtcc atccatatta atctggtttc agttttcggt atatgtcatg aggaaatatt
     5821 acagacatat tctggtgcga ggaaaagaaa ccgaaaccat ttcagaaagg ttgatacgtg
     5881 aggctgatac acgagaatat gaactatggc gtccggtccg tcgctttttc tccattgttt
     5941 tcctcattgt tctattaggt tgcattcatt cactgataac aactataaaa taaaagttat
     6001 ggctgttctg atacaggaaa ataaacgttc attctatggt tacctcttcc tgttatatag
     6061 aagaatttta aacgacaagt aagggaatat aaatgaaaat aacaaaaaat aaaaatgcaa
     6121 ctataaaaaa cagtcatcag tcggctgctt ttcaatgttt accttatcat tttc
//
LOCUS       DAZGTR010000206         6116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGTR010000206 DAZGTR010000000
VERSION     DAZGTR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.208"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            74..850
                     /gene="exuR"
                     /locus_tag="ACHLCY_004685"
     CDS             74..850
                     /gene="exuR"
                     /locus_tag="ACHLCY_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP0371775.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1090..1173
                     /gene="yqiM"
                     /locus_tag="ACHLCY_004686"
     CDS             1090..1173
                     /gene="yqiM"
                     /locus_tag="ACHLCY_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP0371776.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1195..1857
                     /gene="yqjA"
                     /locus_tag="ACHLCY_004687"
     CDS             1195..1857
                     /gene="yqjA"
                     /locus_tag="ACHLCY_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP0371777.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1861..2244
                     /gene="mzrA"
                     /locus_tag="ACHLCY_004688"
     CDS             1861..2244
                     /gene="mzrA"
                     /locus_tag="ACHLCY_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP0371778.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2391..2759
                     /gene="yqjC"
                     /locus_tag="ACHLCY_004689"
     CDS             2391..2759
                     /gene="yqjC"
                     /locus_tag="ACHLCY_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP0371779.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2797..3102
                     /gene="yqjD"
                     /locus_tag="ACHLCY_004690"
     CDS             2797..3102
                     /gene="yqjD"
                     /locus_tag="ACHLCY_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP0371780.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /gene="yqjE"
                     /locus_tag="ACHLCY_004691"
     CDS             3105..3509
                     /gene="yqjE"
                     /locus_tag="ACHLCY_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0371781.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /gene="yqjK"
                     /locus_tag="ACHLCY_004692"
     CDS             3499..3798
                     /gene="yqjK"
                     /locus_tag="ACHLCY_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP0371782.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4376
                     /gene="yqjF"
                     /locus_tag="ACHLCY_004693"
     CDS             3984..4376
                     /gene="yqjF"
                     /locus_tag="ACHLCY_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0371783.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4446..5432
                     /gene="yqjG"
                     /locus_tag="ACHLCY_004694"
     CDS             4446..5432
                     /gene="yqjG"
                     /locus_tag="ACHLCY_004694"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP0371784.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5725..6090
                     /gene="yhaH"
                     /locus_tag="ACHLCY_004695"
     CDS             5725..6090
                     /gene="yhaH"
                     /locus_tag="ACHLCY_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0371785.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDHSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1531 a         1515 c         1543 g         1527 t
ORIGIN      
        1 tatatgacca atggcatacc catgaataaa gtggtatgat aacttcgtcc gcattccact
       61 ccgggagacg cacatggaaa tcactgaacc acgccgtttg tatcaacaac ttgccgctga
      121 cctgaaagag cgcatcgaac agggcgtcta tctggtaggt gataaactgc ctgcagaacg
      181 ctttattgcc gatgaaaaga acgtcagccg tacggtagtt cgtgaagcca tcatcatgct
      241 ggaagttgaa ggctatgtcg aagtgcgtaa aggttcaggt attcatgtgg tttccaacca
      301 gccgcgccat caacaggcgg ctgacaataa tatggaattc gccaattacg gtccgtttga
      361 gttgcttcag gctcgccagc tcatcgaaag taatattgcc gagttcgcgg caactcaggt
      421 aacgaaacaa gacatcatga aactgatggc cattcaggaa caggcgcgcg gcgaacaatg
      481 cttccgtgat tccgagtggg atttacagtt ccacattcag gtagccctgg cgacgcagaa
      541 ctccgccctg gcggccatcg ttgaaaaaat gtggacccaa cgtagccata acccgtactg
      601 gaaaaaactg cacgaacaca ttgattcccg taccgtcgat aactggtgtg atgaccacga
      661 tcaaatcctc aaggcgctga ttcgcaaaga tcctcatgct gctaagctgg caatgtggca
      721 gcacctggaa aacaccaaga tcatgttatt caacgaaacc agcgacgact tcgagttcaa
      781 tgccgaccgc tatctgttcg ccgaaaaccc ggtagtgcat ctcgatacgg ccaccagcgg
      841 cagtaaatga aatacctgtc cgacaggcgc tgcgacgcgc ctgtttgtct cgtcggtaag
      901 cgaaaagtat aaagtgtcag cctgtgtaaa tcctctcgcc accctcccct gcattcagca
      961 aaatcagact ccacggacat gtaattttga taacgaacaa cgttgacctt tgttacaatt
     1021 agattcaatt tgaatttatg tttttgaatg ctttcttatc tcacgattta acagggaata
     1081 gttcaggctg tgttgatgta tcaaacccgc agaacatacc aaaacagcaa taacattgcg
     1141 gtagtgcatc ttttaaaacc agcgtggcgt taaccgattc accaggaata atgaatggaa
     1201 cttttgaccc aattgctgca agccctgtgg gcgcaggatt ttgaaaccct ggccaatcca
     1261 tcgatgattg gcatgttgta ttttgtcttg tttgtaattt tgttccttga aaacggcttg
     1321 cttccagcgg cctttttacc gggcgacagt ttactggtat tggtcggcgt gttgattgcg
     1381 aaaggcgcga tgggttaccc gcaaacgatt ctgctgctga ccgttgccgc cagcctcggc
     1441 tgctgggtca gctatattca ggggcgatgg ctgggcaata cccgcaccgt acaaaactgg
     1501 ctatctcatt tacccgcgca ttatcatcaa cgcgcacacc atctttttca taaacacggt
     1561 ttatcggcgc tgttaattgg tcgctttatt gcgtttgtca gaacactgct gccgacgatt
     1621 gccgggttat cagggctgaa taacgcgcgc tttcagtttt tcaactggat gagcggtctg
     1681 ctgtgggtat tgatcctgac aactctgggt tacatgctcg gcaaaacgcc ggtattttta
     1741 aagtacgagg accagctgat gtcatgcctg atgctgctcc cggtggtgct gctggtgttt
     1801 ggcctggcag gttctctggt cgtgttatgg aaaaagaaat atggaaatcg ggggtaaggg
     1861 atgcaaatac ctcgcatgtc gcttcgccag ctagcctggt ccggcactgt tttacttctg
     1921 gtcggcacgc tgttgctggc ctggtccgcg gttcgccagc aagagtctac gctggcgatt
     1981 cgtgccgttc atcaaggcac aacgatgcca gacggttttt cgatctggca tcaccttgac
     2041 gctcatggca ttcctttcaa aagtatcacc cccaaaaacg acactctgtt aattacattt
     2101 gactccagcg accagagcgc cgccgcaaaa gcggtcctcg acagaacatt gccccatggc
     2161 tacatcattg cgcagcagga caataacagt caggctatgc agtggctgac ccggttacgg
     2221 gataactctc atcgcttcgg ataacttcct gttatccgaa acatatcact cactttggtg
     2281 atttcaccgt aactgtctat gattaatgag gcggtggtta acactaccgc ttcgttgtct
     2341 ggacaatcgt tcctttgtaa taggtccgaa tcacaatgga aggttcaaga atgaaatacc
     2401 gcatcgcttt agctgtttct ctctttgctc ttagtgccgg tagttatgcc actaccctgt
     2461 gtcaggaaaa ggagcaaaat atccttaagg agatcagcta tgccgaaaaa caccaaaacc
     2521 agaatcgtat tgacggtctg aataaagccc tgagtgaagt ccgggccaac tgttcagata
     2581 gccagctgcg tgccgatcat cacaagaaaa tcgcaaagca gaaagatgag gtggcggaac
     2641 gccagcaaga tttagccgag gcgaagcaaa aaggcgatgc cgataagatt gccaaacgcg
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg cattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactaattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat ctttccgcca gtcgtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gcgtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccctcaaatt
     3841 tcttgaagat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaataccg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 gcattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgccg ctggtgattc
     4141 tgcttgagtt cggtggtggt ctggcaatcc tgttcggttt cctgactcgc accacagccc
     4201 tgtttactgc gggctttacg ctgctgacgg catttttatt tcacagcaac tttgctgaag
     4261 gcgtcaactc gctgatgttc atgaaaaacc tgacaatttc tggcggattc ctgctgctgg
     4321 caattaccgg tccgggcgcg tatagcatcg accgcctgct gaataaaaag tggtaagcac
     4381 gcgctatact taacgataaa aaacgaggag gaagctcctc gtttttgcta ttggaggaga
     4441 gaaaaatggg gcaactgatt gacggcgtct ggcatgacac ctggtacgat accaaatcta
     4501 ccggcggtaa atttcaacgt tcagcttccg catttcgtaa ctggctcact gccgatggcg
     4561 cacctggccc cactggcaca ggcggtttta tcgcagagaa agatcgttat catctctatg
     4621 tttcactcgc ctgcccgtgg gcgcaccgca cgctgatcat gcgcaaactc aaaggactgg
     4681 aaccgtttat ttccgtttcc gtagtgaacc cgctgatgct ggaaaacggc tggacctttg
     4741 atgacagttt tccgggagca accggagata cgctctatca acatgaattt ctgtatcagc
     4801 tttatctcca cgccgatcca cactacagcg gacgagttac tgttcccgtg ctgtgggaca
     4861 aaaagaacca caccatcgtc agcaacgaat cagcagaaat catccgcatg ttcaataccg
     4921 cgtttgatgc gctgggcgca aaagcgggtg attactaccc accagccctg caaacgaaaa
     4981 ttgacgaact taacggctgg atttatgaca ccgttaacaa cggcgtgtat aaagctggtt
     5041 ttgccaccag ccagcaagcc tacgacgagg cggtggcgaa agtgtttgaa tcgctggcgc
     5101 gactggagca gattttaggc cagcatcgtt acctgaccgg caaccagcta accgaagccg
     5161 atattcgcct gtggaccacg ctggtccgtt ttgatccagt gtatgtgacc cacttcaagt
     5221 gtgataagca ccgcatcagc gattacctta atctgtatgg cttcctgcgt gatatctacc
     5281 agatgccggg aattgccgaa acagtcaatt tcgatcacat ccgtaatcat tacttccgca
     5341 gccataagac catcaaccct acggggatta tttcaattgg tccatggcag gatctcgatg
     5401 aaccgcatgg acgagatgtt cgcttcggtt aaaaaaaggc accttacgag gtgcctctga
     5461 taattagcta agtgcatgtt gttcatgtcg gatgcggcgt gaacgcctta tcaaacctac
     5521 aaaacattta tattcaatat gttgcaatga ccgcgcaggc cagataagcg tagcgcatct
     5581 ggcgattttg cgcttgtcat cagtcttagc gcgccttcac agcactttat taattccttg
     5641 aacgaatatt tactgccatt tcattcacgt ctattcttaa tttgctgctt taaaaacaag
     5701 tgattgagca aaattgaggc aaaaatggac tggtatctga aagtactaaa aaattatgtc
     5761 ggtttccggg gtcgtgcgcg gcgcaaagag tactggatgt ttattctggt caacatcatc
     5821 tttacgttcg tgctggggct gctggataaa atgttaggct ggcaacgcgc tggtggcgaa
     5881 ggcatcctga cgacaatcta cggtattctg gtgtttttac cgtggtgggc agttcagttc
     5941 cgccgcctgc acgacaccga ccactcggcg tggtgggcac tgttgttctt aatcccgttt
     6001 atcggctggc ttattatcat cgtctttaac tgccaggcgg gtacgccagg cgaaaaccgc
     6061 tttggaccag atccgaaact ggaaccttaa aacaaatctg ctcgccggat gtctga
//
LOCUS       DAZGTR010000207         6104 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGTR010000207 DAZGTR010000000
VERSION     DAZGTR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.209"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            457..906
                     /gene="alaE"
                     /locus_tag="ACHLCY_004696"
     CDS             457..906
                     /gene="alaE"
                     /locus_tag="ACHLCY_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP0371786.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPANILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(943..1287)
                     /locus_tag="ACHLCY_004697"
     CDS             complement(943..1287)
                     /locus_tag="ACHLCY_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP0371787.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1439..1768
                     /locus_tag="ACHLCY_004698"
     CDS             1439..1768
                     /locus_tag="ACHLCY_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP0371788.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2016..2261
                     /gene="nrdH"
                     /locus_tag="ACHLCY_004699"
     CDS             2016..2261
                     /gene="nrdH"
                     /locus_tag="ACHLCY_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP0371789.1"
                     /translation="MRITIYTHNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2258..2668
                     /gene="nrdI"
                     /locus_tag="ACHLCY_004700"
     CDS             2258..2668
                     /gene="nrdI"
                     /locus_tag="ACHLCY_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP0371790.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2641..4785
                     /gene="nrdE"
                     /locus_tag="ACHLCY_004701"
     CDS             2641..4785
                     /gene="nrdE"
                     /locus_tag="ACHLCY_004701"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0371791.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELASCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4795..5754
                     /gene="nrdF"
                     /locus_tag="ACHLCY_004702"
     CDS             4795..5754
                     /gene="nrdF"
                     /locus_tag="ACHLCY_004702"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0371792.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTNELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5908..>6104
                     /locus_tag="ACHLCY_004703"
     CDS             5908..>6104
                     /locus_tag="ACHLCY_004703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371793.1"
                     /translation="MTKNINFSRFALSPYITGNSGDLLASIFMPHLPSGVASDSQYVR
                     VEKSDYFHWVIYRHKQIKESF"
BASE COUNT         1570 a         1507 c         1466 g         1561 t
ORIGIN      
        1 ctctgctcgc taattccgca agcctgacaa aaccgaagtg aataaaaatg caatataaca
       61 gaataaaaga aaacacccct cactggtgct ttctgctgtt aatgtacatt ttctggcata
      121 aaaaatgctg taaatgaccc ggtcacgaca tacaccgaat tctctacgct tatagcgtta
      181 cctcaccccc aaactttcat tagtgaaatt aaaggcaagg tcatatccaa ttgatttaat
      241 tgaaaataaa tcatatcggc atgtaaaaat ccacagtgta caaaaaacga gccgttacgg
      301 aatattttat ctacaaaaac tgactaaata aaaatttttc actaattgaa tagtcatagc
      361 cagcgatata cgctatgcga aaatgcagat ggcaatgaga tccactgctt tcatctccat
      421 taacatccca ttacgctttt attaaggagc attagcatgt tctcaccgca gtcacgcttg
      481 cgtcatgcag ttgcagatac gttcgcgatg gttgtttact gttctgtcgt gaacatgtgt
      541 attgaagttt tcctctccgg aatgagcttc gaacagtctt tttattccag attggtagcg
      601 attccggcga acatcttaat tgcatggcca tacggtatgt accgcgatct gtttatgcgc
      661 gcggcacgca aagttagccc gtcgggctgg ataaaaaatc tggctgatat cctggcttat
      721 gtgacgttcc agtcaccggt gtatgtggcg atcttgttag tggtgggcgc agactggcat
      781 cagattatgg cggcggtcag ttcaaacatc gttgtttcga tgttgatggg ggcggtttat
      841 ggctacttcc tcgattattg ccgccgactg tttaaagtca gccgttacca gcaggtaaaa
      901 gcctgactct tcttttcgcg actggcatcg ccagtcgcgt ctttaactgg cttcgccaaa
      961 taaaccattc aaataacgtt caagcgcaac acgcgaacta aagccatgcg ggataccgta
     1021 atgctcgtgt cgctcgcctg ctaaatagag cggaaattgc tggtaatgat cgcaagtttt
     1081 aatatcggaa gcgggttcag ccagtgttga actgcgttct tttaatgttg gatgaattac
     1141 cagagcggta cgtcccattc gcgcttcgcg attaacatag acataatttt ccccacggcg
     1201 gtaaccatac gctttctggg ttaccacatc gacagtaaaa ccgacttttt caagtacgcg
     1261 cgccacctcg tctggtcgta aatacatatt tgatcctcgt tattatcaac cgcgggccta
     1321 ccttacctga ttgcgcattc acaacgcttt cagaaaagtc cataaagccg cgatgaacct
     1381 cagttaagaa aattatggtc tacactgaaa attacatcga attctaatgg gggatcatat
     1441 gtttaaccga ccgaaccgca acgacgttga tgatggcgtg caggatatcc agaatgatgt
     1501 caatcaatta gctgacagcc tcgaatctgt attgaagtcc tggggcagcg acgccaaagg
     1561 ggaagctgaa gccgcacgca gtaaagctca ggcattgctg aaagaaacca gggcgcgaat
     1621 gcatggtcgt actcgcgtcc agcaagccgc acgcgatgcc gttggctgcg cggattcttt
     1681 tgttcgtgaa agaccctggt gtagcgtggg tacagcagct gcggtaggta tttttatcgg
     1741 cgcactgtta agcatgcgca aatcgtagtg caaaaatgat aataaatacg cgtctttgac
     1801 cccgaagcct ggcttcgggg tttctttttg cctggtgaat cacaaaaatc cccctacccc
     1861 gtcacgctca tatccagggt aatttcgacc actatttgct atatattgtg tggttgaatc
     1921 ttttttcaac tacatctagt atctctgtat caacagagag acaacccgac gcgtatcatc
     1981 gcgccgtatc ttcattttaa acggaaatac gaatcatgcg cattactatt tacactcata
     2041 acgattgcgt tcagtgccac gccaccaaac gggcgatgga aaaccggggc tttgattttg
     2101 aaatgattaa tgtcgatcgc gttcctgaag cagcggaagc gttgcgtgct cagggctttc
     2161 gtcagttgcc ggtagtgatt gctggcgatc ttagctggtc tggtttccgt ccggacatga
     2221 ttaaccgtct gcatccagcg ccacacgcgg ccagtgcatg agccagctcg tctacttctc
     2281 cagcagctcc gaaaacacgc agcgttttat cgaacgttta ggtctgcccg cggtgcgcat
     2341 cccgctcaat gagcgggaac ggattcaggt agacgagcct tacatcctga tcgtgccctc
     2401 ttacggcggc ggcggtacgg ctggcgcggt gccacgacag gtaattcgct ttttaaacga
     2461 cgagcacaac cgggcgttgc ttcgcggcgt tattgcttct ggtaatcgca actttggtga
     2521 ggcgtatggc cgcgccggag atgtgattgc ccggaaatgc ggcgtgccgt ggctgtaccg
     2581 ttttgaactc atgggtacgc aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt
     2641 ttggcaacga caaccgcaga atgcctgacg caggaaacga tggattacca cgcgctgaat
     2701 gcgatgctta acctctacga tagcgcaggt cgcattcagt tcgataaaga ccgccaggcc
     2761 gttgacgcct ttattgcgac gcatgtgcgt ccgaacagtg tgaccttcag tagccagcag
     2821 cagcgcctga actggctggt caacgaaggt tactatgatg aaagcgttct taatcgctac
     2881 tctcgcgact ttgtcattac gctgtttgcc cacgcacaca ccagcggttt tcgtttccag
     2941 acattcctcg gggcatggaa gttttacacc agctatacgt tgaagacatt cgacggtaaa
     3001 cgttatctgg aagattttgc cgatcgagtc acgatggtgg cgctgacgct ggcacaaggc
     3061 gatgcgacgc tggcgttgca actgaccgat gaaatgctgt caggacgctt tcagccagcc
     3121 acgccaacat tcctcaactg cggtaagcag cagcgcggcg aactggcttc ctgttttctg
     3181 ctgcgtattg aagacaatat ggagtcgatt ggccgggcgg taaattccgc actgcaactg
     3241 tcgaaacgcg gcggcggcgt ggcatttttg ctgtcgaatc tgcgggaagc gggcgcgcca
     3301 attaaacgta ttgaaaatca atcttctggc gtaattccgg tgatgaaaat gctggaagac
     3361 gcattttcct atgccaacca actcggcgcg cgtcaggggg ctggtgcagt ctatttacat
     3421 gctcatcatc ccgatattct gcgttttctc gacacgaaac gggaaaatgc cgacgaaaaa
     3481 atccgcatta aaacactgtc gcttggcgtg gtgatcccgg atatcacttt ccatctggca
     3541 aaagagaatg cgcagatggc gctgttttcg ccctatgacg ttgagcgagt ttatggcaag
     3601 ccgtttgccg atatcgccat tagcgaacac tatgacgaac tggttgccga tgaacgcatc
     3661 cgcaaaaaat acctcaacgc ccgtgatttc ttccagcgac tggcagaaat ccagtttgag
     3721 tccggctatc cctacatcat gtatgaagac acggtaaacc gtgctaaccc tatcgccggg
     3781 cgcataaata tgagtaatct ctgctcagaa attttgcagg ttaacagcgc ctcagagtat
     3841 gacgagaatc tcgactatgc ccgtacaggc catgatattt cctgcaattt aggttcgttg
     3901 aatattgccc acaccatgga ttcccccaac tttgcccgca cggtagagac tgccgtacgc
     3961 ggtttaacgg cggtgtcaga tatgagtcat atccgcagcg tgccgtccat cgaagccgga
     4021 aatgccgcct cgcacgccat cggactgggg cagatgaatt tacatggtta tctggcgagg
     4081 gaaggcatcg cttatggttc gccggaagcc ctggatttca ccaatctcta tttctatacc
     4141 atcacctggc acgcactgcg tacctcgatg ttgctggcgc gcgaacgcgg tgaaaccttc
     4201 gccgggttca aacagtcacg ctatgccagt ggtgaatatt tcagccaata tctgcaaggt
     4261 aactggcagc cgaaaacggc gaaagttggc gaactgtttg cccgcagcgg tattacgtta
     4321 cctacccgtg agatgtgggc gcaactgcgc gatgacgtga tgcgctacgg catatacaac
     4381 cagaatctcc aggcggtgcc gccaaccggt tctatctctt atatcaacca tgctacgtcg
     4441 agtattcatc cgattgtggc gaaagtagag atacgcaaag agggcaaaac aggacgcgtt
     4501 tactaccctg ccccgtttat gactaacgag aatctggcgc tgtatcagga tgcttacgaa
     4561 atcggcgcag aaaagatcat cgacacctac gcggaagcga ctcgccatgt cgatcagggt
     4621 ttgtcgctga cgctgttttt ccccgatacc gccaccactc gcgatatcaa caaagcgcag
     4681 atttacgcct ggcgcaaagg tatcaaaacg ctctattaca ttcgcctgcg tcagatggcg
     4741 ctggaaggca ctgaaattga aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa
     4801 ctctcacgta tcagcgccat caactggaac aagatatctg acgataaaga tctggaggtg
     4861 tggaatcgcc tgaccagcaa tttctggcta ccagaaaagg tgccactgtc gaacgatata
     4921 cctgcatggc agacattaag tgtcgtagaa caacaactga caatgcgcgt ttttactggc
     4981 ctgacgctgc tcgacacgct gcaaaatgtt atcggcgcgc cttcgctgat gcccgatgcg
     5041 ctcacgcctc atgaagaggc agtattatcg aatatcagct ttatggaagc ggttcatgcc
     5101 cgctcttaca gttcgatttt ctcgacgctg tgtcagacca aagatgtcga tgccgcctac
     5161 gcctggagtg aagaaaacgc accgttgcag cgaaaagctc agattattca gcaacattat
     5221 cgcggtgatg atccgctgaa aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc
     5281 tattccggtt tctggctgcc gatgtatttt tccagccgcg gaaagctgac caataccgca
     5341 gatctgatcc gtctgattat ccgcgatgaa gcagtccacg gttactacat aggctataaa
     5401 tatcagaaaa acatggaaaa gatatctctg ggacaacgtg aagagttgaa gagtttcgcc
     5461 ttcgatttgt tgctggaact ctacgacaac gagttgcaat acaccaatga gctgtacgcc
     5521 gaaaccccgt gggctgacga tgtgaaagcg tttctctgtt acaacgccaa taaggctttg
     5581 atgaatctgg gctacgaacc gttatttccc gcagaaatgg cggaagtgaa tccggcaatc
     5641 ctcgccgcgc tttcgccgaa tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc
     5701 tcttatgtga tggggaaagc ggttgaaaca gaagatgaag actggaattt ctgagggtgt
     5761 tattttcaaa aatatcacta cccgcagcag ggaaataatt cccgccaaat agctttttat
     5821 cacgcaaata ttctgtagtg atctacactg atactctgtt gcattattcg cctgaaacta
     5881 caatattcag gcgttttttc gctatctatg acaaaaaata tcaacttttc tcgatttgct
     5941 ctcagccctt atatcacggg aaattccggc gatttgctcg catcaatatt catgccacat
     6001 ttgccctcag gggttgcctc agattctcag tatgttaggg tagaaaaaag tgactatttc
     6061 cattgggtaa tatatcgaca taaacaaata aaggaatctt tcta
//
LOCUS       DAZGTR010000208         6077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGTR010000208 DAZGTR010000000
VERSION     DAZGTR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.210"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..62)
                     /locus_tag="ACHLCY_004704"
     CDS             complement(<1..62)
                     /locus_tag="ACHLCY_004704"
                     /inference="COORDINATES: protein motif:HMM:NF017611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP0371794.1"
                     /translation="MVKAMLSPSQSLQYQKESVER"
     gene            complement(43..444)
                     /locus_tag="ACHLCY_004705"
     CDS             complement(43..444)
                     /locus_tag="ACHLCY_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371795.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANMTYMKAYKKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            complement(519..1193)
                     /locus_tag="ACHLCY_004706"
     CDS             complement(519..1193)
                     /locus_tag="ACHLCY_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0371796.1"
                     /translation="MATKIAVETLSPITHNQIPVITTELLAHLYGTDVANIKMNHSRN
                     QTRFLEGKHYFKIVGDDLKNLRVTFSYLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDCYFSQCKKNTGKQEKKPNGLSAKETDSLVWLWDYANRSQALFRELYPALK
                     LIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIYEPDGPTNLLAWERLKNKELP
                     PSLHRY"
     gene            complement(1450..1638)
                     /locus_tag="ACHLCY_004707"
                     /pseudo
     CDS             complement(1450..1638)
                     /locus_tag="ACHLCY_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            complement(1635..2060)
                     /locus_tag="ACHLCY_004708"
     CDS             complement(1635..2060)
                     /locus_tag="ACHLCY_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371797.1"
                     /translation="MMNDFILHETNKSQFWEILKEIIKTGKRWRIKISEYREKRTLSQ
                     NSLLWMWNTEIAAQLSAVSAENFTPEEVHEWLKDIFCPVKKVTIFNITRCVKSTRQLD
                     IGEMHKYLTDIDQWAHRKGMRLTIPDDCEYRDVKRRQEE"
     gene            complement(2060..2278)
                     /locus_tag="ACHLCY_004709"
     CDS             complement(2060..2278)
                     /locus_tag="ACHLCY_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371798.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFRLNNEFQRNRIIQQLDFDD"
     gene            complement(2364..2633)
                     /locus_tag="ACHLCY_004710"
     CDS             complement(2364..2633)
                     /locus_tag="ACHLCY_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371799.1"
                     /translation="MNKKQLAILEKAWDAQISYALKEQALPIIQTKSKIARQLCDGGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEGEMKQ"
     gene            complement(2634..5540)
                     /locus_tag="ACHLCY_004711"
     CDS             complement(2634..5540)
                     /locus_tag="ACHLCY_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP0371800.1"
                     /translation="MTPSELSDLLWAQVDRVAPHLLPNGKKEGHEWVAGNVNGDKGNS
                     LKVNLSGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVI
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRAVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWKSLADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGDV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFELKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQANPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRT
                     RVKTGVTYTYKDDKRHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRN
                     VEKIGNAECLCIMVDHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKP
                     GILLKRLTRQATCCKMPPLLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRY
                     GIQLFIIDSLMKCGIGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTG
                     KMDVKGSGAITDLTDNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEK
                     QRNGEGWEGGVPLFLDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
     gene            complement(5648..>6077)
                     /locus_tag="ACHLCY_004712"
     CDS             complement(5648..>6077)
                     /locus_tag="ACHLCY_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371801.1"
                     /translation="ADPRTYHSFEPVNEPKNIKFERVRTKCEKSSDRHEETDKAFEEI
                     FWCAGMRKAGKKNAASAFRTQFREWRKTTGGTASEFATMLAEDIACRNGKQFGFDRLL
                     PSSYLNGQRWNDEKPETIQPQARPSSAITVSKTGYVFFDR"
BASE COUNT         1402 a         1587 c         1328 g         1760 t
ORIGIN      
        1 cgctcgacgc tttctttctg gtattgaagg gattgggatg gactaagcat ggctttcacc
       61 attaaaaagt cgcttgtaag catcaatgtc tcgttttgct tcaccgagct ttcgtcttaa
      121 ttccatgttt tctgattcaa gcttttccat gtcttgctgg tatcggtcgc ggtgttcttt
      181 ccatgctttt ttatacgcct tcatgtatgt catgttggcc tttctctttg cctgacgaac
      241 tgcgtggtgg tttttcacaa accagtcagg gtcgttaaat gctgctctgg cgcaggtata
      301 ccaataattt gttgcctccc tgtttagcca ataaatactg ataaatggca accggataga
      361 caccattttt cgttgagact ctttctcgcc aaacatgtgc ccttttttga tgctaaggcc
      421 aaatccaggt tgaattaaaa gcattgtcat gtcatctcac attgaaaatt cagcaataaa
      481 aaacccagcc gaagctgggt ttgttaagtt gtcaattgtc agtagcgatg cagtgaaggc
      541 ggcaactctt tgttcttaag cctttcccat gccagaaggt tcgtcggccc gtcaggctca
      601 taaatatcta tatcccgcgt gtgattaatt aaaacgcccc tcgccctccc gatgatatac
      661 gagaactcat agccgtagtc gtggcatatg ccggaatagc cagactgaat cagttttaat
      721 gcgggataca actcacggaa caatgcctgt gagcggttgg cataatccca cagccataca
      781 aggctgtctg tttcttttgc ggaaagcccg ttgggcttct tctcttgttt gccagtattt
      841 ttcttgcact ggctgaaata gcagtcttcc agtttttcga acacttccca cgcctgatcg
      901 gtttcgagca ttttggcgtg acgggctgct ccgcgttctg tccagaggat gagggagcgg
      961 gttttggggg aaatttgcag gtaactaaaa gttactcgca aatttttcag atcatcgcca
     1021 acgattttga aataatgctt cccttccaga aaacgagttt gattacgtga atgattcatt
     1081 tttatgttgg caacatccgt tccataaaga tgcgccaata gctcggtggt tatgacaggg
     1141 atctggttgt gggtaatcgg ggaaagagtt tcaactgcga ttttggtggc cataagatga
     1201 tcctttttct ttgtgaatca tcaccacctg cgacgccaat cgcttggtgg tgaactgtgc
     1261 agggttggcg taaccgggaa aaaggaaccg gcgcggattg ctccgccccc acacagccca
     1321 ccattgagat gtgactgtgc aaacgacaat aaaaaagacg ctggcgcgtc tgttgtcgcc
     1381 tttttcatcc gggacgccaa tcccgacgcc agattttgct ggcgtgtgag gaatatagcc
     1441 ccggataaat catcgcgtca atccccttgt attcctcgca cgatgtctta gccaccggat
     1501 atcccacagg tgagccgtgt agttgaaggt ttttacgtca gattattttg ggattggctt
     1561 gcgtttattt ctggagcgtt tcgttggaag gtatttgcag ttttcgcaga tgatgtcggt
     1621 gatacttcgt cgcgtcattc ctcttgccta cgtttcacat cccggtactc acaatcatct
     1681 ggaatcgtca gccgcatccc cttccgatgc gcccactggt caatgtctgt cagatattta
     1741 tgcatttccc cgatatcaag ctggcgcgtt gatttaacgc atcgcgtaat attaaaaatc
     1801 gtcacctttt ttaccggaca aaatatatct tttagccact catgaacctc ttcaggcgtg
     1861 aagttttcag cagaaacagc agataactgt gcggctattt ctgtattcca catccacaga
     1921 agactgtttt gtgacaatgt acgcttctca cggtactcag atatttttat gcgccagcgt
     1981 tttccggttt taattatttc tttaaggatt tcccagaatt gtgatttatt agtttcgtgc
     2041 agaataaaat cattcatcat cagtcatcaa aatcgagttg ttggataatt ctgttgcgct
     2101 gaaactcatt gttgagtctg aacaaccgtc gaagaacacg gtcacgcgga tagcgccgtg
     2161 cggcaggtga atgctcatac aactcatcaa gcggcaaact ggacgatgaa cgataccgat
     2221 accaacgcac caactcttca cgaaaattag ccctgacaag ctcagctatc gtactcattt
     2281 cttaaagcct ccaatccctc tcccccaaat aaaaaggcct gcgattacca gcaggcctgt
     2341 tacaagctca gtgatgtaga tggtcattgc ttcatctccc cttccatttc atcaatgtca
     2401 acgtcatcag gaagatggga acaatacgct gctataccat gatgatttat ctcataccct
     2461 ttgaacgtta ccatctggcg cgtaatctca acttcgttca ggaatccgcc atcgcataac
     2521 tgcctggcta ttttcgattt ggtctggatt attggtagtg cctgttcttt caaagcgtat
     2581 gatatttgtg catcccatgc cttttcgaga atggctaatt gttttttatt catttaatac
     2641 tccgtcacgt tttcctgtcg ccacgcctcg tcatattccg atttcggcat attggcgatg
     2701 tagctatatg gcgatcctga ttcaagttgc aggaactggt gcgattgctc gtcaaggaac
     2761 aacgggacac caccttccca accttcgccg ttacgttgtt tttcaagcat caaaacagat
     2821 gccggagatg ccagtagctg ttcgtccttc tctgacatct tttcaccact ctgaactctc
     2881 tgtaacgctc tctcgcgagc cttgttacgc cagatgatga aaaggttgtc tgtcaggtct
     2941 gttatcgctc cagagccttt tacgtccatt ttcccggttg gtttttcttc gctgtctcct
     3001 tttcgcgagt gagtaacgag aatgacgtgg gagtttgttt tgtttttgaa gtcgcaaatc
     3061 gagtcaacaa acgccttctg cccgttatag tcatcgtcgc ctatgccaca tttcatcagg
     3121 ctgtcgatga tgaataactg gatcccgtat cggcggcgag cgtagtcgaa tatttcgatc
     3181 agcctgtcgg ctttcgccgt tccggtcagg ccaaacaccc aaagtctttc gtcataaaat
     3241 ttaaatgcag agtcaatttc cagcaatgga ggcatcttgc aacacgttgc ctgacgggta
     3301 aggcgcttaa ggagaatacc aggcttcagc tcaagtgacg cgatgcacgt cttcacaccc
     3361 tgacgcattg cctcaagtgc catatgcccg acaacctcgg tgttatgcgt cgccacatat
     3421 cccctggtta catacaggtg gcgagggtga tcgaccataa tacacaagca ttctgcattg
     3481 ccgatttttt caacattcct gacgaatacc ccacagccct cgaatcgatg cgcagttaat
     3541 ctgccattga gtcgtggtga acggatggcc tccctgattt ctcttgtcag tctgattctt
     3601 gcctcgtatg aatccatccc atgccgctta tcgtccttgt atgtgtatgt cacgccagtt
     3661 ttaactcgcg tccggcatga cccgccgagt gagttaacca gttgaacaac cccattgcgc
     3721 agttcttcac ttgctgagga aaaaacaagc gttccatcct tctcgacata cccatccgtt
     3781 tcaagcagac cacacagcat gcctatacgc gttgatttat ttgcggaaaa aaacacacga
     3841 gggatgaatt tgttttttgc tgtgcacccc attagtccat aacctcgcag ggtctccatg
     3901 agtggatttg cctgaccacg cgccgttgat atcagccagt ccttaccatc tccagagaag
     3961 ttgtaatcag gcagttcagc cttcatacgc tcgatcatgt atggctcgac gtttgaaaac
     4021 ttcacgctcc cattgctaag actaccatcc ccgagaaggg agccgataac ccatgctaat
     4081 ggctctgagt ggtcgccaaa gtcaccagtt atttcaggaa tcctaacgcc atttttgtgc
     4141 ctcttcgttt cactcaaccg cttcagctcg aaggtgtcaa tcacgcggcg tttctcgcct
     4201 ttcgtgaatc cacgactagt gacctcccat aggtgatcgc ctgcgcaatc aacataacga
     4261 ccatcttcaa atgtgactcg gtaaacatct ctaacaccct gcgggaatat cccagtgaca
     4321 gttgacggat tgccgtctac tgacgccacc tgatcgccaa tttttacatc cccgtgagta
     4381 gtccatgtcc catcagctaa aagaataggc tcattcagtg ggcatgcctt tccgtgaccg
     4441 ttcacaccat tgaccagcgt caactctgcc tcacggaact ggaatttatc tgccagagat
     4501 ttccacggtg gattaaacag atactgctgc ttgccgtaga aagcgttgat agtgtcctgg
     4561 taaaactctc gcgcgctgta gagttcttcg gggtcgaagt aggatgccgt gccgatgtac
     4621 tgccagattt catcctcggt aacaccgttc atcaggcatt cgttgatgtc tttgtacggc
     4681 agtgtaacaa gacggcaacg atgttcaccg agtcggcttg cgatttccct tgcggcttca
     4741 cgaccaacat catcaacgtc catcgagatg aatatttcct caaacctgtc gaggttgtga
     4801 tactcaaact caatccactg ttgcttagca cctttcccgc caccaaacgg cacggataac
     4861 gccgagatgc cgtattgcgc atagctcata caatcaattt cgccttcgca aagtacaacc
     4921 gccctcacgc cagcgtccag agcctgccat ccgaacagac aaggttcgca atcaccttct
     4981 gccataatga ctttcttccc gtccgggcgc tcagtgctga ttcgcttgac ctgcaacaac
     5041 tcaccatcgc gtttgtacgg aatcaccaga gcatccagtt ctcgttctcc attccacacc
     5101 ttgccgctga caacctcgta gcgctttacg acttctggcg atatgccacg cgattgcagg
     5161 tactcaagat gggattctgt tctggtaacg tagcgggcga ttttcttgcg atcaggtctg
     5221 gagaatttct tctcacgtct ggcatcgaaa tggtgatcgt catccttgat accgagaaat
     5281 gctttcgctt cctgcatagc ctgatgcagg ttaattccac gacatgccat ccacaaatca
     5341 agcatgtcac cgccgtctcc ctcagcgaaa tcagcccatt ttttcttgcc gctaaggttg
     5401 accttaaggc tgtttccctt gtcaccgtta acgttaccgg caacccactc atgcccctct
     5461 ttcttgccgt ttggcaacag gtgcggagcc accctgtcaa cctgcgccca aagcaggtcg
     5521 ctaagttcac ttggcgtcat gattccctca gattgagatt tttaaaccag aaatcgacaa
     5581 acgaaatact taaccagccg tggttataac cagcgaccag tagcgatttg atttttgatt
     5641 tcatggttca cctgtcgaaa aacacgtagc cagttttcga tacggtgatt gcggatgatg
     5701 gtctggcttg tggttgaatg gtttctggct tctcgtcgtt ccagcgctga ccgttcaggt
     5761 agctcgatgg taacaacctg tcgaatccga actgcttacc attcctgcat gcgatgtctt
     5821 ctgccagcat cgtggcaaac tcgcttgccg tacccccggt agttttacgc cattccctga
     5881 actgtgttct gaatgccgaa gctgcgtttt tcttcccggc tttccgcatg cctgcacacc
     5941 agaatatttc ctcgaatgcc ttgtcggttt cttcgtgacg gtcagatgat ttttcgcact
     6001 tcgttcggac acgttcgaac ttaatgtttt taggttcatt gactggttca aaagagtgat
     6061 aggttctggg gtcagct
//
LOCUS       DAZGTR010000209         6024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGTR010000209 DAZGTR010000000
VERSION     DAZGTR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.211"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            189..1340
                     /gene="yiaY"
                     /locus_tag="ACHLCY_004713"
     CDS             189..1340
                     /gene="yiaY"
                     /locus_tag="ACHLCY_004713"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP0371802.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1505..3043
                     /gene="aldB"
                     /locus_tag="ACHLCY_004714"
     CDS             1505..3043
                     /gene="aldB"
                     /locus_tag="ACHLCY_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP0371803.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3262..3465)
                     /locus_tag="ACHLCY_004715"
     CDS             complement(3262..3465)
                     /locus_tag="ACHLCY_004715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371804.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRE"
     gene            3588..3911
                     /locus_tag="ACHLCY_004716"
     CDS             3588..3911
                     /locus_tag="ACHLCY_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0371805.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3917..5053
                     /locus_tag="ACHLCY_004717"
     CDS             3917..5053
                     /locus_tag="ACHLCY_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0371806.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKGTRDKFAKEYKRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYLSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(5050..>6024)
                     /locus_tag="ACHLCY_004718"
     CDS             complement(5050..>6024)
                     /locus_tag="ACHLCY_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0371807.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQQPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1504 a         1418 c         1556 g         1546 t
ORIGIN      
        1 gaaaatgatt aattaatttt taaaataaat taatacaaat ttcttatgat tttaaaaaag
       61 cacattgttt aatgaatgca atgtgctttt tattagatta attttgataa ttaataataa
      121 tgatcatgtt ttcgaatgaa aatcctcagt aagctgccca ccctttttta cactttcagg
      181 agtgtgttat ggcatcttca actttcttta ttccttctgt gaatgtcatc ggcgctgatt
      241 cattgactga tgcaatgaat atgatggcag attatggatt tacccgtacc ttaattgtca
      301 ctgacagtat gttaacgaaa ttaggtatgg cgggtgatgt gcaaaaagca ctggaagaac
      361 gcaatatttt tagcgttatt tatgatggca cccaacctaa ccccaccacg gaaaacgtcg
      421 ccgcaggttt gaaattactt aaagaaaata attgcgatag cgtgatctcc ttaggcggtg
      481 gttctccaca cgactgcgca aaaggtattg cgctggtggc agccaatggc ggcgatattc
      541 gcgattacga aggcgttgac cgctctgcaa aaccgcagct gccgatgatc gccatcaata
      601 ccacggcggg tacggcctct gaaatgaccc gtttctgcat catcactgac gaagcgcgtc
      661 atatcaaaat ggcgattgtt gataaacatg tcactccgct gctttctgtc aatgactcct
      721 ctctgatgat tggtatgccg aagtcactga ccgccgcaac gggtatggat gccttaacgc
      781 acgctatcga agcgtatgtt tctattgccg ccacgccgat cactgacgct tgtgcactga
      841 aagccgtgac catgattgcc gaaaacctgc cgttagccgt tgaagatggc agtaatgcga
      901 aagcgcgtga agcaatggct tatgcccagt tcctcgccgg tatggcgttc aataatgctt
      961 ctctgggtta tgttcatgcg atggcgcacc agctgggcgg tttctacaac ctgccacacg
     1021 gtgtatgtaa cgccgttttg ctgccgcatg ttcaggtatt caacagcaaa gtcgccgccg
     1081 cacgtctgcg tgactgtgcc gctgcaatgg gcgtgaacgt gacaggtaaa aacgatgcgg
     1141 aaggtgctga agcctgcatt aacgccatcc gtgaactggc gaagaaagtg gatatcccgg
     1201 caggcctacg cgacctgaac gtgaaagaag aagatttcgc ggttctggcg actaatgccc
     1261 tgaaagatgc ctgtggtttt actaacccga tccaggcaac tcacgaagaa attgtggcga
     1321 tttatcgcgc agcgatgtaa tcatcatttc cacaacggct ggcaaattgt cagccgcttt
     1381 ttcaactatc tctgtaaccc ttgcccgtaa attcgtgata gctgtcgtaa agctgttacc
     1441 gactggcgaa gatttcgcca gtcacgtcta cccttgttat acctcacacc gcaaggagac
     1501 gatcatgacc aataatcccc cttcagcaca gattaagccc ggcgagtatg gtttccccct
     1561 caagttaaaa gcccgctatg acaactttat tggcggcgaa tgggtagccc ctgccgacgg
     1621 cgagtattac cagaatctga cgccggtgac cgggcagctg ctgtgcgaag tggcgtcttc
     1681 gggcaaacga gacatcgatc tggcgctgga tgctgcgcac aaagtgaaag ataaatgggc
     1741 gcacacctcg gtgcaggatc gcgcggcgat tctgtttaag attgccgatc gaatggaaca
     1801 aaaccttgag ctgttagcga cggctgaaac ctgggataac ggcaaaccca ttcgcgaaac
     1861 cagtgctgcc gacgtgccgc tggcgattga ccattttcgc tatttcgcct cgtgtattcg
     1921 ggcgcaggaa ggtgggatca gtgaagttga tagcgaaacc gtggcctatc atttccatga
     1981 accgttaggc gtggtggggc agattatccc gtggaacttc ccgctgctga tggcgagctg
     2041 gaaaatggct cccgcgctgg cggcgggcaa ctgtgtggtg ctgaaacccg cacgtcttac
     2101 cccgctttct gtactgctgc taatggaaat cgtcggtgat ttactgccgc cgggcgtggt
     2161 gaacgtggtc aacggcgcag gtggggaaat tggcgaatat ctggcgacct cgaaacgcat
     2221 cgccaaagtg gcgtttaccg gctcaacgga agtgggccaa caaattatgc aatacgccac
     2281 gcaaaacatt attccggtga cgctggagct gggcggcaaa tcgccaaata tcttctttgc
     2341 tgatgtgatg gatgaagaag atgccttttt cgataaagcg ctggaaggct ttgcactgtt
     2401 tgcctttaac cagggtgaag tttgcacctg tccgagtcgt gccttagtgc aggagtcaat
     2461 ctacgaacgc tttatggaac gcgccatccg ccgtgtcgaa agcattcgca gcggtaaccc
     2521 actcgacagc gtgacgcaaa tgggcgcgca ggtttctcac gggcaactgg aaaccatcct
     2581 caactacatt gatatcggta aaaaagaggg cgcagacgtg ctcaccggcg ggcggcgcaa
     2641 gctgctggaa ggtgaactga aagacggcta ctacctcgaa ccgacgattc tgtttggtca
     2701 gaacaatatg cgggtgttcc aggaggagat ttttggcccg gtgctggcgg tgaccacctt
     2761 caaaacgatg gaagaagcgc tggagctggc gaacgatacg caatatggcc tgggcgcggg
     2821 cgtctggagc cgcaacggta atctggccta taagatgggg cgcggcatac aggctgggcg
     2881 cgtgtggacc aactgctatc acgcttaccc ggcacatgct gcgtttggtg gctacaaaca
     2941 atcaggtatc ggtcgcgaaa cccacaagat gatgctggag cattaccagc aaaccaagtg
     3001 cctgctggtg agctactcgg ataaaccgtt ggggctgttc tgatataaga agctggtcgc
     3061 attgggtatt cattgcctga tgcgacgctt acgcgtctta tcatgcctac gggaacctga
     3121 ccgtaggccg gataaggcgt acacgccgca tccggcataa acaaagcgca cgttgttaca
     3181 atttacgttt taaacctacg tgcgcaataa cccagactac gccaacagaa gtgcgtttct
     3241 ggggcgtgtt ttgccaggag tttactctcg cgtttcgatg gtgagcgggc aaaccaggcc
     3301 gtaaatcacc attggcgttc cgaggccaat cagcaataaa cggtagataa ttgaaggttt
     3361 aatacgcttt atctgtactg tggatgtttt actggagtta ttcattagct tggtcaatag
     3421 ttccattatt tcaatatgat aaacctcgac ctgttgaaaa gccacgcagc acacttcaga
     3481 aagtatataa aaaacaatat gccgttatat aaataaggaa attgcactgc atcatgggat
     3541 ttcataatat gcgttaattg ttctttttta tattctctga gggaaatatg tttctggatt
     3601 attttgcact gggagtgctt atttttgtat ttctggtgat attctatggg attattattt
     3661 tacatgatat tccctacctg attgctaaaa aacgtaatca tcctcatgcc gacgccattc
     3721 atgttgctgg ttgggtgagt ctttttacgc tgcatgttat ctggccattt ttgtggattt
     3781 gggccacgct ttaccgcccg gagcggggat ggggaatgca aaaccatgac tcctccgttg
     3841 tgcaactaca acagcgtatt gccgggctgg agaaacagct cgccgacatc aaatcctctt
     3901 ctgccgagta atacttatgg atttactgat tattttgacc tatgtggctt ttgcatgggc
     3961 aatgtttaag atcttcaaaa ttcctgtaaa taaatggacc attcccacag cggccctggg
     4021 tggcatattt attgtcagtg gtttaattct gttaatgaac tataaccatc cgtatacctt
     4081 caaagcgcaa aaagcggtta tttctattcc tgttgtccca caggtgacag gcgtggtgat
     4141 cgaagtgacg gataagaaaa atacgctgat taaaaaaggt gaggtgctat ttcgactgga
     4201 cccgacgcgt tatcaggcgc gggtggatcg gctgatggcg gatatcgtta ccgcagaaca
     4261 taaacagcgg gcgttgggcg cagagttaga tgagatggcg gcgaatactc agcaggcaaa
     4321 gggcacgcgg gataaattcg ctaaagagta taagcgttac gcacgtggca gccaggcgaa
     4381 agtaaacccg ttttcagaac gcgatatcga tgtggcgcgg caaaattatc tggcgcagga
     4441 agcctccgtg aagtcatcgg cggcggaaca aaaacagatc cagagccagc tggatagcct
     4501 ggtgttgggt gaacattctc aaatcgccag cctgaaagca cagctcgcgg aagcaaaata
     4561 taaccttgag cagacgatag tgcgtgcgcc gagcgatggt tatgtgaccc aggtactgat
     4621 tcgtccgggt acctatgccg cgtcgctgcc gctacgtccg gtgatggtgt ttatacccga
     4681 tcagaaacga caaatcgtgg cgcagttccg tcagaactcc ttgctgcgac tggctcctgg
     4741 cgacgatgcg gaagtggtgt ttaatgctct gccaggtaag gtattcagcg gtaagctggc
     4801 agccattagt ccagccgttc ccggcggagc ttatctgtcg accggcacct tacagacgtt
     4861 aaacacagcg ccgggttcag atggcgttat cgcgaccatt gaactggatg agcacactga
     4921 tttgagcgct ttaccagacg gtatttacgc ccaggtggcg gtttactctg atcatttcag
     4981 ccatgtctcg gtgatgcgca aagtgctatt acgcatgacc agctgggtgc attaccttta
     5041 tctcgatcat taatcttcgc tttccgccgc ttgttcaggc cgcagttgct gcgactgagc
     5101 aagctggtgg cgaatttcac gcagcatttc atccagcaca tactggaccc gccagggctg
     5161 gtattcccgt ttacggaaga tagccaccag atccagccac cattcatagg gttgctgaaa
     5221 gcagggcacc agcgtgccgt cttgcaggtc gctctgcaca cttttgtctg gcgcaaacac
     5281 aatccccagg tgatttcgtg ccagctccag cgcagattgc gtgttgtcgc aaacgtaatt
     5341 tcccttcacg cgataatccc gcacctcttt actcccggcg acgttgaagc gccagatatt
     5401 cgcgtcgtcg atcatcatcg agtcgatcaa aatacaggag tgatgttcga gttcgtcagg
     5461 acggctaatg ggatgtttct caagatagcg ctggctggca taagcggtta cggcatattg
     5521 ggtaataaaa ctggcaacca gcgattcatc ttttggtgga gcgtagctga ttaaaacatc
     5581 acaatcatca ggaaattcga cgccttcata aaaggcatta cgctcaagat tgcaggtttt
     5641 tagcgataag gtgatatcac cgatatcttt aattttatcg attacatgtt tggataaata
     5701 ggtaattatg ccggttgggg cgtagatggt gacccgacca cgtttctcat gcttataatc
     5761 tgcaataaaa ttattaagtt gctcgtttct gtccagcatg tcgttgatgt acggtaacag
     5821 cgcggtgcca aaaggtgtga gcatcagctg ccgggtggtt cggtcaaaaa cttttaaacc
     5881 cacttttgat tcaaaatcag caagatattt gctgacgttg gcctgtgcga tgccaagtac
     5941 agtcgcggca tggctgatat tttcactggc agcgattacc gagataattt ttaactcccg
     6001 gtacttaagt tgtaattttg tcat
//
LOCUS       DAZGTR010000210         5972 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGTR010000210 DAZGTR010000000
VERSION     DAZGTR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.212"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(69..830)
                     /gene="kduD"
                     /locus_tag="ACHLCY_004719"
     CDS             complement(69..830)
                     /gene="kduD"
                     /locus_tag="ACHLCY_004719"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP0371808.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(860..1696)
                     /gene="kduI"
                     /locus_tag="ACHLCY_004720"
     CDS             complement(860..1696)
                     /gene="kduI"
                     /locus_tag="ACHLCY_004720"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP0371809.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1983..3170)
                     /gene="yqeF"
                     /locus_tag="ACHLCY_004721"
     CDS             complement(1983..3170)
                     /gene="yqeF"
                     /locus_tag="ACHLCY_004721"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP0371810.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIE
                     RDE"
     gene            3425..4654
                     /gene="yqeG"
                     /locus_tag="ACHLCY_004722"
     CDS             3425..4654
                     /gene="yqeG"
                     /locus_tag="ACHLCY_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0371811.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLFSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5114..5746
                     /gene="yqeH"
                     /locus_tag="ACHLCY_004723"
     CDS             5114..5746
                     /gene="yqeH"
                     /locus_tag="ACHLCY_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0371812.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPSLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         1653 a         1431 c         1258 g         1630 t
ORIGIN      
        1 gattatgatg atctacaggt ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga
       61 agaatgaatt aacgcgccag ccaaccgcca tccacggcaa tggtataacc attcacataa
      121 tctgaagcgc tggaggcaag gaacactatc ggccccatca ggtcactcgg cagtccccaa
      181 cgaccagctg gaatgcggtc gagaatttcc gcgctacgtt gttcatctgc ccgtagttgt
      241 tgagtattgt tggtcgccat gtaacccgga gctatcgcat taacattaat gttgtgttta
      301 gcccattcgt tcgccatcaa tcgcgtcaca cccatcacgc cgctttttga tgcggtataa
      361 gaaggcacac ggatcccgcc ctggaaggag agcattgacg cgatattgat aatcttgccg
      421 ccattgcctt gcgcgataaa gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg
      481 atattcaggt tcatgacatc gtcccagtcc ttttcgctga actcgagagc atcttcgcgg
      541 cgaatcaatc cggcgttatt caccaggata tcaatatgac caaactccgc taccgcgcga
      601 tccagcagtc caggaatgcc atcaatcttt cgcagatcgg cggtcaggct taaaaaacga
      661 cgccccagcg ccgtgacctg ctcgatggtt tcagtcggtt caacgatgtt aatgccaaca
      721 atgtcacagc ccgcttgcgc cagccccaac gccatcccct ggcccagccc agtatcacaa
      781 ccagtgacga ccgcaacttt accttcgaga gaaaatgcac ttaaaatcat aacaatacct
      841 tattcgttta tgcccacaac tagcgcaaat ctttaacggc cacatggtcc atatcatcaa
      901 agacctggtt ttcaccgacc atgccccaga taaaggtata agctttggtt ccgacgccgg
      961 aatgaatcga ccagctcggg gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg
     1021 tttcttgcgg ctgccccatc atgtggaaaa cgcaggcgtc atcatccata ttgaaataga
     1081 aataaacttc catccggcgc tcgtgagtgt gacacggcat ggtgttccac aagttacctg
     1141 gagccagctc cgtcagcccc atactcaatt ggcaggtttc cagcacatcc gggacaaaat
     1201 atttgttaat tgtgcgacgg ttactggtga ggttatcgcc taacgtgact ggagatactt
     1261 cgtccggtgt gacttttttg gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa
     1321 actttgccgg agtggcggta tcaatactgg caaagacaac ttcttttgca cctttaccca
     1381 catacagggc gtcgcggtga ccgatttcat agcattggcc atcgacagta atcgtaccgg
     1441 caccgccaat attgataaca cctaactcgc gacgttcaag gaaatagctt acgccgagtt
     1501 gtttaccaac ttccccgcca acggaaaccg ttttagttat cggcataatg ccgccaacaa
     1561 taattcggtc aatgtggctg taaaccatgg tgtactcatc ggcgacaaat acctcttcaa
     1621 ccaaaaattc attgcgcagc ccttgggtat ccagcgtttt tgcgtgcgcg ctgtggatgc
     1681 tctgtcttac gtccacatca acctccgata atcagtgaag cgtgaaaata gaaaacgata
     1741 gtgaaacact gtttcgtttt ctctataaag ggatgatatg cccatgggct agggttttca
     1801 attaaaaata aaacaatgtt tcatttttaa gttaaggatt aaaaaaagtg cagtgtcgat
     1861 cacgaatatg tcatgagaat ggaaaaatga gggagcccag aaaatgtatg tcgacttgcc
     1921 agccatcatt attcaacaga ttaatgacgg cagatattta tcggtaagga ggaaatgcag
     1981 agctactcgt cacgttcaat ggtcaatgcc acgccctggc ccccgccgat acaaagcgtt
     2041 gccagccctt tgcgggcatt acgtttcacc atttcatgaa ccagagaaac caggattcgg
     2101 caaccggaag cgcctatcgg gtgaccgagt gcgatcgcgc caccattgac attgacattg
     2161 acccgacgct catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct
     2221 tcattagcct cgataagatc gacatcagcc aactgccagc ctacacgctc caggcaacgg
     2281 cgggtcgcat acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat
     2341 gcgcggatgc gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc
     2401 attaccgcag ctgcgccatc gtttatggat gatgcattcc ccgcggtcac agaaccgaga
     2461 ctatcaaatg aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt
     2521 tcatcggtat caacaaccaa cgtctgaccg ttactttgga tcattaccgg gacgatctca
     2581 tctttaaatc gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa
     2641 gcatcctgca actgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca
     2701 atatgataat cattgaaggc atcccacaac ccatcatgca ccagactgtc aaccaactgg
     2761 ctattgccaa gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg
     2821 ttttcctggc caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc
     2881 agatgcagtg ctttaagccc agaaccgcaa acgtcattaa tagtgattgc agaaacgcta
     2941 ttaggaagac cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc
     3001 aacacctgac caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt
     3061 aacgctttca cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccgcga
     3121 aagcagccga taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg
     3181 caccaattaa cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa
     3241 aaagtgattt ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac
     3301 gtaaggtttg caaccactaa cccaccaata gaggggtaga tagggcgtta atctcccata
     3361 cttaacctgg tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca
     3421 gataatgtct aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac
     3481 agccgttggt gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt
     3541 cctgtttatt actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg
     3601 ccagttcatc ctctcatcga aaacatcagc aggtgaaggg ataacgggcg cagtaacaca
     3661 ctactatggc aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt
     3721 cgtcgtgttg atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca
     3781 tatggttatt gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taatcctgaa
     3841 tctcattttt ctgatgggac gccatgcaac cattcgggta atgggttttt tggtattccc
     3901 attgattgcc tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct
     3961 attaacgacc caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc
     4021 cgtgatggtt ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg
     4081 tgaaaaatat ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct
     4141 catcatctgc ataagtgtac tgttctttgt ctttagctgc ctgttttcta ttccaccttc
     4201 gtatattgaa gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc
     4261 gaacgcccca gcatggctgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa
     4321 atcattcctg ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac
     4381 actacagcag gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt
     4441 ggtatcgctg atcaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta
     4501 cgcgatcagc ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta
     4561 tctgatcccg gcgcttaaac cctggcgttc catcggaaat ctgattacgc tgatcgtggg
     4621 tatcctgtgc gtatcggtaa tgttctttag ctgatatcca ttgtgaatgg ttcaaaattg
     4681 ctacaatttt gaaccattcc agttttcata atgttcattc gttattattc cgcactatca
     4741 cttccctcat catatcctag tattatcagt tagttaaaca cacaaagaga tctgaacatc
     4801 ttaaataaga ctatttaaga tgcataactt agattcgcaa gatatcttcc ggtcattcaa
     4861 aaacaatttg cgatatagat attctcatac catgagatag ttatctgaac caccctcaac
     4921 caggtaagat taaatcagtg aaacataatc atatttaata acgagaaatg cattttaaaa
     4981 tgtcttaatc agaattatag caaatacaga ttaatcgcaa tttttcccag caaaattaca
     5041 aaatcatggt gtattgacat catcaatcac caatgaaatt atgcaattat atacggatag
     5101 ggaggttctt aacatggggc tttgtagtcg ttataaaagt cttacatgca atagttgctc
     5161 aatgcattgc caaataatgc cagaagagtc accgagtttg cagtactgcg caaactcgtg
     5221 tttttgtatg tggcccgaag aaagttcata ttttaatcgt ggtgtggtag aaggtatctt
     5281 aacaaaaaac cacaatgcga gattaagcgg atatattttt gtcgattttt cagtaagttt
     5341 tttacgtcta ttccttgaaa aagactggat tgactatctt gcaagtactg acatggggat
     5401 tgttttagtc agcgaccgca atatgcaatc gttagctaat tactggcgaa aacacaactc
     5461 agcaatatct gctgtcatat ataatgatga tggtcttgat gttgccaatg aaaagatcag
     5521 acaactattt attggtcgtt atttatcatt taccagaggt aacacattaa ctcagatgga
     5581 attcaccata atggggtata tggtttctgg ttataatcca tatcagattg ctgaagttct
     5641 ggatatggat atccgtagca tctacgcgta caagcaacga atcgaaaaga gaatgggtgg
     5701 taaaataaac gaattattta ttcgttcaca ttcggtccaa cattgatact caacgaccag
     5761 ccagaatcat actctgttta tacgggcatt tattgaaatg tccgtatttt ataaataggc
     5821 cgtcccctca agagtaaaca ccatagtatt taaaaccatc acacgaaaaa ttcagaagca
     5881 ttacgaaata tgaatttaca aacaatagtg gcataaatgt taaccatgtt aatttacgta
     5941 aagttttacg ttgcaacatt aaagcctcat tt
//
LOCUS       DAZGTR010000211         5876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGTR010000211 DAZGTR010000000
VERSION     DAZGTR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.213"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..370
                     /gene="cesT"
                     /locus_tag="ACHLCY_004724"
     CDS             <1..370
                     /gene="cesT"
                     /locus_tag="ACHLCY_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFP0371813.1"
                     /translation="EIYYISLSDASDEYMMIYGVCGKFPTDNPNFALEILNANLWFAE
                     NGGPYLCYESGAQSLLLALRFPLDDATPEKLENEIEVVVKSMENLYLVLHNQGITLEN
                     EHMKIEEISSSDNKHYYAGR"
     gene            428..3235
                     /gene="eae"
                     /locus_tag="ACHLCY_004725"
     CDS             428..3235
                     /gene="eae"
                     /locus_tag="ACHLCY_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type gamma"
                     /protein_id="HFP0371814.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLSAGLGLFFYVNQNSFANGENY
                     FKLSSDSKLLTQNVAQDRLFYTLKTGETVSSISKSQGISLSVIWSLNKHLYSSESEML
                     KAAPGQQIILPLKKLSVEYGALPVLGSAPVVAAGGVAGHTNKMTKMSPDATQSNMTDD
                     KALNYTAQQAASLGSQLQSRSLHGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPENMLGYNV
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQLRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTEHSTQKIQLIVKSKYGLDRIVWDDSALRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADGTEAITYTATVKKNGVTQANVPVSFNIVSGTATLGANSATTDANGKATVTL
                     KSSTPGQVVVSAKTAEMTSALNASAVIFVEQTKASITEIKADKTTAVANGNDAVTYTV
                     KVMKEGQPVQGHSVAFTTNFGMFNGKSQTQNATTGSDGRATITLTSSSAGKATVSATV
                     SGGNDVKAPEVTFFDGLKIDNKVDILGKNVTGDLPNIWLQYGQFKLKVSGGNGTYSWH
                     SENTNIATVDESGKVTLKGKGTAVINVTSGDKQTVSYTIKAPNYMIRVGNKASYANAM
                     SFCGNLLPSSQTVLSNVYNSWGPANGYDHYRSMQSITAWITQTEADKISGVSTTYDLI
                     TQNPHKDVTLNAPNVYAVCVE"
     gene            complement(3524..4744)
                     /gene="escD"
                     /locus_tag="ACHLCY_004726"
     CDS             complement(3524..4744)
                     /gene="escD"
                     /locus_tag="ACHLCY_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP0371815.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPIEFWIDGQPTPYEADQPLPIGKVIDIAGCGFIIGDIDHALP
                     LSDMPERFSARNQRKKRLILAGVIGAAIALSGAIGSYVLLSPKAELPTFTRADVYQQL
                     KENKLYAITLVWHGKNIALYGRCESTTDLTPFFNYLKENNIFYYNNIICNNQIISAIN
                     DILTEYGYKDIIITKGNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNN
                     KINELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILF
                     QNIQPYISADIFPGKILRISGTMTNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGIN
                     ISRPDEYIHIPLSY"
     gene            4887..>5876
                     /locus_tag="ACHLCY_004727"
     CDS             4887..>5876
                     /locus_tag="ACHLCY_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFP0371816.1"
                     /translation="MANGIEFNQNTASIFNSNSLDFELESQQLTQKSSSNSNSPLLNL
                     QNELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQELLTLTDSDKLKE
                     LSLKNSGLLEQHDPNLAMFGNMPKGEIVALISSLLQSKFVKIELKKKYAKLLLELLGE
                     DDWELALLSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEVKDLPEREKH
                     LKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRIIVFLSLDNYTDIIAISIKKDKDVI
                     LNEVLSIIEHVWLTEDWLLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDN"
BASE COUNT         1769 a         1082 c         1265 g         1760 t
ORIGIN      
        1 tgaaatttat tatatttcgt tatctgatgc cagtgacgaa tatatgatga tttatggcgt
       61 ctgtgggaaa ttcccgacag ataaccctaa cttcgctctt gagattttga atgcaaactt
      121 atggtttgca gagaatggtg ggccatacct gtgttacgag tcaggagcac aatcgttgtt
      181 gttagcgtta cgtttcccgc tcgatgatgc tacccctgaa aaacttgaga atgaaataga
      241 agtcgttgtt aagtcaatgg aaaacctgta tttggtatta cataatcagg gaataacatt
      301 agaaaacgaa catatgaaga tagaggaaat tagctcaagc gataataaac attattatgc
      361 cggaagataa aatctattta ttaatctaat ttactactca ttctaactca ttgtggggac
      421 cccaaacatg attactcatg gtttttatgc ccggacccgg cacaagcata agctaaaaaa
      481 aacatttatt atgcttagcg ctggtttagg attgtttttt tatgttaacc agaactcatt
      541 tgcaaacggt gaaaattatt ttaaattgag ttcagattca aaactgttaa ctcaaaatgt
      601 tgctcaggat cgcctttttt atacgttgaa aacaggtgaa actgtttcca gtatttctaa
      661 atcacaaggt atcagtttat ccgtaatttg gtcactgaat aaacatttat acagttccga
      721 aagcgaaatg ctgaaggctg cgcctggcca gcagatcatt ttgccactca aaaaactgtc
      781 tgttgaatat ggtgccttac ctgtcttagg ttcggcacct gttgttgctg caggtggtgt
      841 cgctgggcat acaaataaaa tgactaaaat gtccccggac gcgactcaaa gcaacatgac
      901 tgatgacaag gctctaaatt atacggcaca acaggccgcg agccttggta gccagcttca
      961 gtcgcgctct ctgcacggcg attacgcgaa agataccgct cttggtatcg cgggtaacca
     1021 ggcttcgtca cagttgcagg cctggttaca acattatgga acggcagagg ttaatctgca
     1081 gagtggtaat aactttgacg gtagttcact ggatttctta ttaccgttct atgattccga
     1141 aaaaatgctg gcatttggtc aggtcggagc gcgttacatt gactcccgct ttacggcaaa
     1201 tttaggtgcg ggtcagcgtt ttttccttcc tgaaaacatg ttgggctata acgtcttcat
     1261 tgatcaggat ttttctggtg ataatacccg tttaggtatt ggtggcgaat actggcgaga
     1321 ctatttcaaa agtagcgtta acggctattt ccgcatgagc ggctggcatg agtcatacaa
     1381 taagaaagac tatgatgagc gcccagcaaa tggcttcgat atccgcttta atggctatct
     1441 accatcatac ccggcattag gcgccaagct gatgtatgag cagtattatg gtgataatgt
     1501 tgctttgttt aattccgata agctgcagtc gaatcctggt gcggcgaccg ttggtgtaaa
     1561 ctatactccg attcctctgg tgacgatggg gatcgattac cgtcatggta cgggtaatga
     1621 aaatgatctt ctttactcaa tgcagcttcg ttatcagttt gataaaccgt ggtctcagca
     1681 aattgagcca cagtatgtta acgagttaag aacattatca ggcagccgtt acgatctggt
     1741 tcagcgtaat aacaatatta ttctggagta caagaagcag gatattcttt ctctgaatat
     1801 tccgcatgat attaatggta ctgaacacag tacgcagaag attcaattga tcgttaagag
     1861 caaatacggt ctggatcgta tcgtctggga tgatagtgca ttacgtagcc agggcggtca
     1921 gattcagcat agcggaagcc aaagcgcaca agattaccag gctattttgc cggcttatgt
     1981 gcaaggcggt agcaatattt ataaagtgac ggctcgtgcc tatgaccgta atggcaatag
     2041 ctctaacaat gtacagctca ctattaccgt tctgtcgaat ggtcaggtgg tcgaccaggt
     2101 tggggtaacg gactttacgg ctgataagac ttcggctaaa gcggatggca ccgaggcgat
     2161 tacttatacc gcgacggtga aaaagaatgg ggtaactcag gctaatgtcc ctgtttcatt
     2221 taatattgtt tcaggaactg caactcttgg ggcaaatagt gccacaacgg atgctaacgg
     2281 taaggcaact gtaacgttga agtcgagtac gccagggcag gtagtcgtgt ctgctaaaac
     2341 cgcggagatg acttcagcac ttaatgccag tgcggttata tttgttgagc aaaccaaggc
     2401 cagtattact gagattaagg ctgataagac aactgcagta gcaaatggta atgatgctgt
     2461 tacatacact gttaaagtga tgaaagaggg tcagccagtg cagggacact ccgttgcatt
     2521 cacaacaaac tttgggatgt tcaacggtaa gtctcagacg caaaatgcga ccacgggaag
     2581 tgatggtcgt gcgacgataa cactgacttc cagttccgca ggtaaagcga ctgttagtgc
     2641 gactgttagt ggtgggaatg atgttaaagc acctgaggtt acattttttg atggactgaa
     2701 aattgacaac aaggttgata ttcttggtaa gaacgttact ggtgacttac ctaatatctg
     2761 gttgcaatat ggtcagttta aactgaaggt aagcggtggt aatggtacat attcatggca
     2821 ttcagagaat accaatattg cgactgttga tgaatcaggg aaagtaacct tgaaaggaaa
     2881 aggtactgca gtaattaatg ttacatctgg tgataagcaa acagtaagct acactattaa
     2941 agctccgaat tatatgataa gagtgggtaa taaagccagt tatgcaaatg ctatgtcctt
     3001 ttgtggaaat ttattaccat cctcacagac ggtattatca aacgtttata attcatgggg
     3061 gcctgcaaac ggatatgacc attatcgttc tatgcagtca ataacagctt ggattacaca
     3121 aactgaagct gataaaatat caggagtatc aactacttat gacttaataa cacaaaaccc
     3181 tcataaggat gttacgctaa acgctccaaa tgtctatgca gtttgtgtag aataatttaa
     3241 aatcctttcc ccggctaaaa atatattatt ttagctgggg aattattgtt gtttctttat
     3301 tatcttcttc ttaataagta acgtcctaga atcaacatct cctgttggtt aataagttaa
     3361 tctcaaagaa acatattcac gtttcctcat gtagcgactc gactactttc aacgagccaa
     3421 cctcgctaat accaaagctg aatcacatca agcaacaact atgctcacaa catccataca
     3481 ataaataaaa aaaccctccg aagaggggga agatgacaag acgttaatac gatagcggaa
     3541 tatgtatata ttcgtctggg cgggagatgt tgataccatc tttaggatca atcgcgtcta
     3601 tgacatagcc tcctttcaga atgctaccaa ttcccaggct tgtaccgtta tcaagagcaa
     3661 ttgttggatt cgtcatcgtg ccgctgattc tgagtatttt tccaggaaag atatctgctg
     3721 aaatgtacgg ttgaatattc tgaaatagta tctttacgtt actatttttg ttcattgcat
     3781 ttataatggc aaggatctta ctctcgttct gttgagaaac ttcccccgca acaatgatga
     3841 catcattctt tttgaaaata ttgacatagt ttattaattt attcttctta aactctgatg
     3901 ccagttcatt gattttattg ttactgttgt tgtgtatttc ccatccagca actcccggcg
     3961 tattcagtaa cagattttca acttcgctcc atttgggtga agggggaatg tatcccgata
     4021 acagaaagaa tccaggctta ttaccttttg taatgatgat atctttataa ccgtattccg
     4081 ttaaaatatc attaattgcg gatatgattt ggttattgca aatgatatta ttgtaataaa
     4141 aaatattatt ttctttcagg taattgaaaa atggagttaa atccgtggtt gattcgcaac
     4201 gcccataaag cgcgatattt ttcccatgcc ataccagggt gattgcataa agtttgtttt
     4261 cttttagctg ctgatataca tcagctctgg taaacgttgg gagttcagct tttggggaaa
     4321 gcaaaacgta actgccgatg gctccagaca acgcaatagc ggcaccaatc acgcctgcca
     4381 ggataagacg ctttttgcgt tgattcctgg cagagaacct ttctggcata tcagacaatg
     4441 gcagggcgtg gtcaatatca ccaatgatga agccacaccc cgctatatcg atgacctttc
     4501 cgataggtaa tggttgatct gcttcatagg gagtgggctg tccatcaatc caaaattcaa
     4561 ttggagataa aaggaaaaca ccttcctcac gtatatcaag taaaaactga ttgaggcctt
     4621 gttcaagggg aaagtaaaca atatcgttat cttctgtgcc tatcgttagg tttcccatcg
     4681 gaagttgtaa ttcccgatta cgcattgcac cattgagaag ttttatttta tatgaggata
     4741 acatttttaa attttattcg tcctggtggt tgaacaatga gaaaaaatat ggtgaactta
     4801 cactgactaa gaatagagta gaaaggaagc tgttgtacag tactatttaa tggaatattc
     4861 ataattaatg tttacgtgag tttccaatgg ctaatggtat tgaatttaat caaaacaccg
     4921 catctatttt caattctaat tcattagatt ttgaattgga atctcaacaa ttaacgcaaa
     4981 aaagctcttc taatagcaat tctccattat taaatttaca aaatgaatta gcgatgatta
     5041 ccagttcgtc gctttctgaa acgattgaag ggctaagcct agggtatcgc aaaggtagcg
     5101 ccaggaaaga agaggaaggt acgacaacgg agaagctgct caatgatatg caagagctgc
     5161 ttactctgac cgatagcgat aaactaaaag aactatcatt aaagaacagt gggctgctgg
     5221 aacagcatga tcctaacttg gctatgtttg gtaatatgcc aaaaggggaa attgtcgcgc
     5281 tcatatcttc tctattgcaa tcaaagtttg ttaagattga attaaaaaag aaatatgcca
     5341 aattattatt agaattatta ggcgaagatg attgggagtt ggctctgctt tcctggttag
     5401 gggtgggtga gttaaatcag gaaggtatcc agaagatcaa gaagctttat gaaaaggcta
     5461 aggacgagga ctctgaaaac ggagcctctt tacttgactg gtttatggag gtcaaggatc
     5521 ttccagagcg tgagaagcac ttaaaggtta ttattagggc gctatcgttc gatctttctt
     5581 atatgtcttc ttttgaagac aaggtaaaaa catcttcaat tattagcgat ttatgccgga
     5641 taatcgtttt tttatcgctt gataactata cagatattat tgcaatctcc attaagaaag
     5701 ataaagatgt cattttaaat gaagtgttat ctattattga acatgtatgg ctaacagaag
     5761 actggctact ggaaagccct tctcgggtat cgatagtcga agataaacat gtttattatt
     5821 tccatttatt gaaagacttt tttgcatcat tacccgatgc ttgttttatt gataac
//
LOCUS       DAZGTR010000212         5649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGTR010000212 DAZGTR010000000
VERSION     DAZGTR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.214"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..117
                     /gene="hokD"
                     /locus_tag="ACHLCY_004728"
     CDS             <1..117
                     /gene="hokD"
                     /locus_tag="ACHLCY_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0371817.1"
                     /translation="ICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            complement(159..338)
                     /locus_tag="ACHLCY_004729"
     CDS             complement(159..338)
                     /locus_tag="ACHLCY_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371818.1"
                     /translation="MPDGKVGIRIVKFGFVHHLFSLLAQQVSGVQADVRIVNQNARKK
                     QNPPKRVKCGCVEDA"
     gene            285..557
                     /locus_tag="ACHLCY_004730"
     CDS             285..557
                     /locus_tag="ACHLCY_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371819.1"
                     /translation="MVHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            559..1614
                     /locus_tag="ACHLCY_004731"
     CDS             559..1614
                     /locus_tag="ACHLCY_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0371820.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRNQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWATINNVADALPETVLRRSLGLRAEKIRSVYRESDIVPGEQTATSMLKQR
                     TKNIALLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPRREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            1615..1980
                     /locus_tag="ACHLCY_004732"
     CDS             1615..1980
                     /locus_tag="ACHLCY_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0371821.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQRVPGGRLGVKIYKIESE"
     gene            1989..2519
                     /locus_tag="ACHLCY_004733"
     CDS             1989..2519
                     /locus_tag="ACHLCY_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP0371822.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            2761..2958
                     /locus_tag="ACHLCY_004734"
     CDS             2761..2958
                     /locus_tag="ACHLCY_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0371823.1"
                     /translation="MLKQQDMTETARVVFNELSVTDPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            3109..4167
                     /locus_tag="ACHLCY_004735"
     CDS             3109..4167
                     /locus_tag="ACHLCY_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0371824.1"
                     /translation="MLNTVKISSCELINADCLEFMRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWAGDEDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDTEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAVLGITAKQIADATGKKNMVSHWFSAGQWLLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVDTYASLNRQYAELQSEYKHLRRYFGVTVQVPYTDVWTYKPV
                     QYYPGKHPCEKTAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLIIRNG"
     gene            4208..4283
                     /locus_tag="ACHLCY_004736"
     tRNA            4208..4283
                     /locus_tag="ACHLCY_004736"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4241..4243,aa:Ile,seq:cat)
     gene            4364..4440
                     /locus_tag="ACHLCY_004737"
     tRNA            4364..4440
                     /locus_tag="ACHLCY_004737"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4398..4400,aa:Arg,seq:tct)
     gene            4964..>5649
                     /locus_tag="ACHLCY_004738"
     CDS             4964..>5649
                     /locus_tag="ACHLCY_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0371825.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAV"
BASE COUNT         1424 a         1333 c         1592 g         1300 t
ORIGIN      
        1 atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg cgaggtacga
       61 atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga ggagtaagag
      121 accaggcgag ggagaaatcc ctcgccacct ctgatgagtc aggcatcctc aacgcacccg
      181 cacttaaccc gcttcggcgg gttttgtttt ttcctggcat tctggtttac aattcgcacg
      241 tcagcctgaa cacctgacac ctgctgcgcc agcagagaaa acagatggtg cacaaaacca
      301 aatttcacaa ttctgatacc gaccttgcca tccggcatgg gcggcgttca cacgcattta
      361 aaaccgactg gtaccaacac ccaccatgta ctgaagaaca ggccgaatgg ctaattcata
      421 actaccgcag acgcggatac gagattaaga aagccctcag cctcgattat cgtcactgga
      481 taatctatgt caggctccct tattccgaac gcccaccgcg tccgtcccgc acattccagc
      541 aacgcatctg gaggtaacgt gcgggtatta cttcgacctg ttccggtacc ggaacttggg
      601 ctggtggtgc taaaaccggg ccgtgaatcc atgcaggtat ttcataaccc tcgagttctg
      661 gtggagccgg aaccgaaaag catgcgcggt ctgccgtccg gagtcgttcc tgctgttcgc
      721 cagccgctgg cggaggataa atcattactg ccatttttca gcgatgagcg ggtgattcgt
      781 gctgctggtg gtgctggtgc attgtctgac tggctgttac gccacattaa atcctgccag
      841 tggcctcatg gtgactacca tcacagtgaa accgtcatac atcgttacgg taccggcgcg
      901 atggtgttgt gctggcactg cgacaaccag ctgcgtaacc agacttccga atcactcggg
      961 cagcttgctc accaaaacct gtcagcatgg atgattgatg tcatccgcca cgcaatgaat
     1021 ggcacgcagg agcgggaatt atcgctggct gaattatcct ggtgggcaac cataaataac
     1081 gtagcggacg cactaccgga gacggtgtta cgtcgttcac tgggattacg cgcggaaaaa
     1141 attcgctcag tataccgcga gagcgacatc gtgccgggag agcagaccgc caccagcatg
     1201 ctgaagcagc gcacaaaaaa tattgcgcta ctgcctcacg cccaccagca aaacccgcca
     1261 caggaaaaga cggtggtaag cattgccgtt gatccggagt caccggctca gtatctccag
     1321 cgccagaaac cacgacggga agagatgcct gtatacacgc gttgggtaaa aacgcagaaa
     1381 tgcatgacgt gtggtaatca ggcagatgat ccgcatcaca tcattggtca tggactggga
     1441 gggatgggaa caaaggctga tgatttgttt gttattccgc tgtgccgtaa atgccatagc
     1501 gaactacacg ccggggtaaa agattttgaa gaaaaacacg gcagccagct gttgttgctg
     1561 attcgttttt taatgcacgc gagaaattcg ggtgtcctga agtggaaagc atgaatgact
     1621 gaacgcatag aatttgtttt gccttacccg ccaacggtga acacttactg gcgacgtcgt
     1681 ggcagcacat attttgtatc aaaagccggt gagcgttatc gccgtgatgt ggcgcttatt
     1741 gttcgccagc agcggctgaa attaaacctg tccggaaggc tggcgataaa gattattgca
     1801 gagccaccgg ataaacgtcg tcgtgacctg gacaatatcc tgaaagcacc actggatgcg
     1861 ctgacgcatg ccggacttct catagacgac gagcagtttg atgaaatcaa tattgtgcgc
     1921 ggtcagcgcg ttcctggggg acggctgggc gtgaagattt acaaaattga gagtgagtga
     1981 gcgtaaatat gatatatccg gaaattacag gcaaaagcgg cgaacattta cgcctgaaca
     2041 cgctggaagc agtctggatc caggggaaat tacggatgtg ggggcggtgg tcgtatatcg
     2101 gtgggggtaa atccggaaat atgtttaacc ggttactggt ttcgaaaaag ctgacgaaaa
     2161 cagcagttaa tgaggtttta cgcagcatga agaaatccgg gctggaaaaa ccggaacttg
     2221 aggcattttt tcgggatatg accagaggga agcagaagag ctggttgtca cattgtacag
     2281 acacagaggc gttgattatt gatcgcgtta tcagtgaggt gcttggggaa tatcccgggc
     2341 taatcaatat tctccggcaa aggtacgaag gacggggaat gagtaagaga aaaatggcag
     2401 aatgtttaaa tcgtactcac ccggaatggt gtttcagcac atgtgagaaa cgtattgcag
     2461 gttggttagc cgtggctgaa cacatgcttt atgtacctat gcacgattca tttcgataaa
     2521 aaaagcttgc ttttttacgc agaaacagct tgaattcctg taagcttcgc aaagctgtat
     2581 cgcgaggcga aatgcaagtt ttttcgcaca aggaagccac cggaaggtgg tttttttgtg
     2641 tccgtaatat acagcagcgc aataaattcg ctggtggtta ttaataccgt tctttcaggt
     2701 tgctggcttt ttcgacaaga gttattggtg tgtcacgtta accggaaaag ggaaaaagac
     2761 atgctgaaac agcaggatat gacagaaacc gccagagtgg tgtttaatga attaagcgtt
     2821 accgacccgg cgacagtcgg ggagattgcg cagaatactt acctttcacg cgaacgctgc
     2881 cagttaatac tgacccagct ggttatggcg ggtctggcag actatcagtt tggttgttac
     2941 agacgccttc agtcctgaag gcttttttat ttgtggtaaa tgggcggctg gtgggtgtaa
     3001 ggggcaccca ccagccatct gctcatgcgt tgggttcaca agcaaacctc aggcccactg
     3061 ctttgcgcaa aagcagaatg agcctatcag agacaggctt aatgatccat gcttaatact
     3121 gtaaaaatat ccagttgtga gttaatcaac gccgactgcc tggaatttat gcggtcgtta
     3181 cccgaaaatt ctgttgacct gatagtcacg gacccgccgt acttcaaagt gaaacccgag
     3241 ggctgggata accagtgggc gggtgatgaa gattacctga agtggctgga ccagtgtctt
     3301 gcgcagttct ggcgggtgct gaaacctgcc ggaagtcttt acctgttctg tggccatcgt
     3361 ctggcatctg acaccgaaat catgatgcgt gagcggttta acgtgctgaa ccatatcatc
     3421 tgggcaaagc cgtccggacg ctggaacggg tgcaacaagg aaagcctgcg ggcgtatttc
     3481 cccgccacag agcgcattct gttcgcagag cattatcagg ggccgtatcg tccgaaagat
     3541 gccgggtatg aggcgaaggg tagggcactg aaacagcatg tgatggcccc gctgattgct
     3601 tactttcgtg atgcgcgcgc tgtcctgggg ataacggcaa aacagattgc agatgccaca
     3661 ggaaagaaaa acatggtgtc gcactggttc agtgccggtc agtggctgct gccgaacgaa
     3721 agcgattatc tgaaattaca ggcactgttt gcccgggtgg cagaagagaa gcatcagcgg
     3781 ggtgaactgg aaaagcccca ccaccagctg gtggatacgt atgcctctct gaaccgacag
     3841 tatgcggagc tgcagagtga atataagcat ctgcggcggt atttcggtgt gacggtgcag
     3901 gtgccgtaca ccgatgtgtg gacgtataaa ccggtgcagt actatccagg gaaacatccg
     3961 tgcgaaaaaa cggcagaaat gttgcagcag ataatcagcg caagcagtcg tccgggagac
     4021 ctggttgcag atttcttcat ggggtcgggg tcgacagtga aagcagcgat ggcgctggga
     4081 cgtcgtgcaa ctggcgttga actggagact gaacgttttg agcagacggt gcgggaagta
     4141 caggatttaa tcattcgtaa cggatgagat tgcggagtta atcatgcgtc gttattattc
     4201 agcaatcggc cctttagctc agtggtgaga gcgagcgact cataatcgcc aggtcgctgg
     4261 ttcaaatcca gcaagggcca ccaaccgcca ctagctcatc aggaaagaac gtcaccctgt
     4321 gcgagattcg gagtccccgg tggcggtcca ttatcggtat tctgcgttgt tagctcagcc
     4381 ggacagagca attgccttct aagcaatcgg tcactggttc gaatccagta caacgcacca
     4441 caccacactt atctgccctg actctctttt gcgggctttt tattacagga aagacaccgg
     4501 acagtgaaat gttaaatgcc tcacaattca ggcagttgac tgttgcctga catgctgagc
     4561 gtttgttaaa aaaatcctgc atgatgaatc cccctgagcg gcggggcata atgacagatg
     4621 tttggttgcg tattgtatag gcaagttgcg gattctgtct ggtcattgca gaattcaccg
     4681 ggaggcaccc ggcatcatgc tgtatacaga gattaggcat atatccaggc ttctcatcgc
     4741 aggagccttt ttacatgcaa aaaaaagccc gagtgggttc gggcaacagc atgagatact
     4801 tgcattgtca tttttatcgt gtggatttta accagggttt ataaggctgc gcaactgcgc
     4861 ggcctttttc gttttgcggg ctgcggttct cctcttttga ttctccttgt ggccggaccg
     4921 tggcccgcaa ctgttgagga aaatcccgga aaggggagga ataatggcat ttaaacacta
     4981 tgacgtggtc agggcggcgt cgccgtcaga ccttgcggaa aagctgacac acaaactgaa
     5041 agagggctgg cagccgtttg gtagtccggt ggccataacc ccttataccc tgatgcaggc
     5101 gattgcagca gaaggtgatg tggtggtcag tggtgcaact gagccggatt ggtactacgt
     5161 catcgtactg gccgggcagt ccaatgcaat ggcttacggg gaagggcttc cgcttccgga
     5221 ttcatacgat gctccggatc cgcgcattaa acagctggcg cgccgcagta cagttacgcc
     5281 gggtggggct gcctgcagat ataacgatat tattccggcc gaccactgcc tgcatgatgt
     5341 gcaggatatg agtacgctga atcatccgaa ggcagacctg agcaaagggc agtacggctg
     5401 tgtcggccag gggttacata ttgccaaaaa actgctcccg tatatcccga ataacgcggg
     5461 gatcctgctg gtaccatgct gtcgtggtgg ttcggcattt acccagggcg cggaggggac
     5521 attcagtgcg gacacggggg ccagccagga ttcggcgcgc tggggtgtgg gtaaaccgtt
     5581 atatcaggac ctgattgcgc gcaccaaagc tgcattacag aagaacccga aaaatgtgtt
     5641 gctggcggt
//
LOCUS       DAZGTR010000213         5635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGTR010000213 DAZGTR010000000
VERSION     DAZGTR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.215"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(47..3571)
                     /gene="nifJ"
                     /locus_tag="ACHLCY_004739"
     CDS             complement(47..3571)
                     /gene="nifJ"
                     /locus_tag="ACHLCY_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP0371826.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     TWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     GRIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            3845..4111
                     /gene="ydbJ"
                     /locus_tag="ACHLCY_004740"
     CDS             3845..4111
                     /gene="ydbJ"
                     /locus_tag="ACHLCY_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP0371827.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4108..4530)
                     /gene="hslJ"
                     /locus_tag="ACHLCY_004741"
     CDS             complement(4108..4530)
                     /gene="hslJ"
                     /locus_tag="ACHLCY_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP0371828.1"
                     /translation="MKKLAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4641..5630)
                     /gene="ldhA"
                     /locus_tag="ACHLCY_004742"
     CDS             complement(4641..5630)
                     /gene="ldhA"
                     /locus_tag="ACHLCY_004742"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0371829.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1313 a         1465 c         1463 g         1394 t
ORIGIN      
        1 cgtccgggct ttttttttag aattggataa tccttatcca gagcatttaa tcggtgttgc
       61 ttttttccgc ttttccggcc atttgtgcca ggaagtcata gcgtttttgc aaatcagctg
      121 cagcatcttt ccataactgt tctgccactt ctggctgctg cgaattcaga cgacggaaac
      181 gttgctcgtg aagtaacgtt tcttccagtg cttctgacgg cgggcgtgaa tccagcgcca
      241 gcggcagttt gccttcatcg gcacgacgag gatcgaagcg atatagcggc cagaagccgg
      301 tagctgtgag ttggcgcatc tggtcatggc tgagtgccag atcgtaacca tgctcttcac
      361 acgggctata agcaatgatc agcgatggcc ccggatacgc ttccgcttcc tgaatcgctt
      421 tcaccgtctg gttcagctgc gcgccgagag aaatctgcgc cacataaaca tgaccgtaca
      481 tcatcatact gacgccaaga tctttacgcg ctttacgttt gccgtgctcg ccgaacttag
      541 ttactgcacc cagcggtgtc gctttcgacg cctgaccacc ggtgttggaa tagcattgcg
      601 tatccagcac cagaatgttg acgttttccg tcaaactcaa tacatgatcc agaccgccaa
      661 agccgatatc gtaagcccag ccgtcgccac caatcagcca gattgatttt tccaccagtg
      721 catctgcgtc acgcagcagt tcatgtgctt cggcaacatc gttgagttgc tggcgtaaag
      781 ctgcgacctg ttctcgacga acctctggcg tagcgtctga tttcaacgcc gtcagtaatt
      841 ccgccgggat tttatcggca aattgatcca gcagacgcag cacgcggaca cggtgttgat
      901 cgaccgtcag gcggaaacca aggccaaatt cggcattatc ttcaaataga gagttcgccc
      961 atgccggccc acgaccgttg gcatcggtgg tatacggtgt agagggcagg ttaccgccat
     1021 aaattgaaga acagccggtg gcgttagcga tcaacatccg gtcgccatag agctgagtca
     1081 gcaatttaat atacggcgtc tcgccacaac cggagcaagc acctgaatat tcaaacagcg
     1141 gtgtaatcag ctgcgatgta cgaatatcaa tacgtcccag tttgctacgg tcgatttctg
     1201 gcaggttgag gaagaaatcg taattgattt tctcttcttc gacatgttcc aggcgagaca
     1261 tcatattgat ggctttaatc tctggattct gacggtcttt cgccgggcaa acttcgacgc
     1321 acaggttaca accggtgcaa tcttccggtg ccacctgcaa gacatatttc tgcccgcgca
     1381 tatcacgcga tttcacatcc agcgaatgca ggctggcagg ggcgttttcc atcgcttcag
     1441 gcggcaccac ttttgcgcga atagctgagt gtgggcaagc ggcaacgcag tggttacatt
     1501 gggtacagag ttcctctttc caggtgggga tctcttcggc aatattacgt ttttcccagc
     1561 gcgtagtgcc catcggccag gagccgtctg gcggcagcgc cgaaacgggg agggcgtcac
     1621 caagcccggc gagcatcgca gcggttacgg ttttcacgaa atcaggagcg gcatcggaaa
     1681 ccactggcgg tcgattggcg ctgtgcggat ttaccggttg caacggaact tcttctacgg
     1741 attcacgcgc cagcgccaga gcctgccaat tgcgttccac cagatcctgg cctttgctac
     1801 tgtaactttt ggcaatcgca ccctgcaatt ctgcgagggc gctatcgcca ggcagaattt
     1861 gcgtcagatg gaaaaaagcc atctgcatga cggtattaat acgggccgcc aggccacatt
     1921 cgcgggcgat tttcgccgcg ttaatcacat agaagcgcgc ttttttctgg tttaacacgg
     1981 cctgaacttc ttgcggcaag cgcgaccaca cttcatctgc gctgtacggt gtgttgagca
     2041 ggaaaatgcc gccaggtttt aaacgctcag ccatctgata tttatcgata aactgcaact
     2101 ggtggcagcc aacaaaatca gcctgggaaa tgagataagc ggaacgaatc ggctgttcgc
     2161 tcactcgaag gtgagaaacc gtcaggccgc ccgccttttt ggagtcgtaa acaaaatagc
     2221 cctgtgcgta ccacggcgtg gaattaccga taatcttgat attgtttttg gtcgcggaaa
     2281 cgctgccatc actaccaagg ccataaaaca aggcttcaag tttcgccgag tttggctggg
     2341 tgttttccgg caacggcagg gacagattgg ttacatcatc gtaaatacca accgtaaagc
     2401 gcgctttcgg tttagccgcg ttgagctcgg caaataccgc cagtacacag tccgggccaa
     2461 attctttgga tgaaagacca tagcgcccac caatgacacg gggcagagtt tcgcgcttgc
     2521 cattattaaa ggcttctgcc agtgcggtca tcacatccag atagagcggt tctgcctggg
     2581 caccgggttc tttggttctg tccagtaccg ccacgctacg tacggatccc ggtagagttt
     2641 gtagcaagtg tttggctgag aaggggcggt acaggcgaac tttcagcacg ccgacttttt
     2701 cgccacgggt tagcaattca tcaaccactt cttcacaggt gccaatggca gagcccatca
     2761 ggataatcac ccgttccgct tgcggatgcc cgtaatattc aaacggctga tactgacgac
     2821 ctgtcgcggc agagaaatca ttcatcgcct gttcaacatg gtcatagacc gcgtcgtacc
     2881 atgggttggt ggcttcgcga gactggaaat aagtgtcagg attggcggac gtaccgcgga
     2941 tcaccggatg ttccgggttg agtgcccggg cgcgatgagc atcaatttcg gcctgcggca
     3001 tgagatcaag aatcgtgtca tcggccagcg ggacaatttt gttgatttcg tgggacgtgc
     3061 ggaaaccatc aaagaaatga ataaatggca cgcggctttt cagcgtcgcg atgtgcgaaa
     3121 tgagagcaaa gtcttgcgct tcctggacgt ttgccgcaca caacatcgcg caacccgtct
     3181 ggcgcaccgc catgacgtcg gaatgatcgc caaaaataga gagtgcatgt gtggcaacgg
     3241 tacgtgccgc tacatgcagg acaaacggtg tgagttcgcc tgccagtttg tacagcgtcg
     3301 ggatcatcag cagcaaaccc tgcgatgacg taaacgatgt tgaaagggca cccgtctgca
     3361 aagcgccatg cacggtagcg atagcacccg cttccgactg catttcaacc acgcgtggtg
     3421 tgtctcccca aacgttcttc aagccgtttc cggcccaggc atcagcctgt tctgccatcg
     3481 tggaactggg ggtaataggg tagatggcga taacttcact ggtgcgaaac gccaccgaag
     3541 caaccgcgcc attaccgtca atagtaatca tatgacaccc ttacattgcg caaatgaggg
     3601 gcgcacgaaa ttgctgcgcg cccagtagta atccttcaat tttagcaaat ggctttcttc
     3661 tgcattttcg cttttgtgtc ccccacatca gcgtaatgaa tgttttgatc aaacagaggg
     3721 caaaaaaata gccataaaaa agtaaaaatg cggggcaacg cgcaggacag ctctcgacgc
     3781 cgccctctgt gctgcctatt atttatggcg gtgtcgtttg gcttgaagat cagaggaaga
     3841 agagatgcga gcagcgtttt gggtagggtg tgccgcttta ttgttgtcgg cgtgtagtag
     3901 tgaacctgtt cagcaggcga ctgcggcgca cgtagcgcca ggtttaaaag cgtcgatgtc
     3961 cagtagtgga gaagcaaatt gtgcaatgat cggcggttcg ctttctgttg cccgtcaact
     4021 ggatggtacg gcgattggga tgtgtgcatt acccaacggc aaacgctgta gcgaacagtc
     4081 acttgccgcc gggagctgtg gcagctatta attcattaaa tccgccagct tataagttaa
     4141 tgtctgtttc gcggtcgcca gcgttaactg gttcgcggtc agatccactt gtgcaccttc
     4201 tttcagcatt tcgctaatgg tgttatcgag ttcattaagc tgcgggttag cgcacatcat
     4261 acgggtcatt gccagccctt tggctgtcag ttcaccatta gacagtttgc cttcaccgct
     4321 aaagcggtta cacatgctgc cggaaatcat cattttttca ccaaagctga tttctggcgg
     4381 atttttatcg ctggtcacgg gcttaccgtt tacgctttcc agcacaaagc gatgatgctg
     4441 tagctgttct ggcgtaacag caattttgtc attacttaca catcccgcca tcagcaggct
     4501 tagcgcaaca aacgcggcta gtttcttcat tgtggttctc aattacagtt tctgactcag
     4561 gactatttta agaatagaga atgaaaggtc attggggatt atctgaatca gctcccctgg
     4621 attgcagggg agcggcaaga ttaaaccagt tcgttcgggc aggtttcgcc tttttccaga
     4681 ttgcttaagt tttgcagcgt agtctgagaa atactggtca gagcttctgc tgtcaggaat
     4741 gcctggtgcc cggtaaacag cacgttgtgg caggcagaca gacgacggaa cacgtcatcc
     4801 tggatcacgt cgttggattt gtcttcaaag aacagatcgc gttcgttctc atacacgtcc
     4861 atacccaacg aaccaatttt ctgatttttc agcgcttcaa ttgctgcctg agaatcaatc
     4921 aatgcaccgc gactggtatt gacgatcatc acgccatttt tcatctgatc gaaggcggct
     4981 tcgttcaaca gatggtagtt ttccggtgtc agcgggcagt gcagagagat aacgtctgat
     5041 tcagagaaca gggttggcag atcgacatac tccacaccga gttccagcgc cgctgcactt
     5101 ggatacggat cgaacgccag cagacgcata ccaaaacctt tcagaatgcg cagcatcgcc
     5161 acaccgattt taccggtacc gataacgcct gccgttttgc catacatagt aaatccggtc
     5221 agaccttcca gagagaagtt agcgtcacgg gtacgctgat acgcgcggtg aatacggcgg
     5281 ttcagcgtca tcatcatacc gatggcgtgt tcagcaacgg cctctggatc ataggctgga
     5341 acacggacta ctttcagccc cagttctttt gccgcgtcaa ggtcgacgtt attgaaaccg
     5401 gcacagcgca gggcgatata tttaacgccg tgctttttca gctcttccag caccgggcgg
     5461 ctgccgtcat cgtttacgaa aatacatacc gcttcgcagc cattggcagt tttagcggtt
     5521 ttttccgtca gcagaaagtc aaaaaattcc agctcaaagc caaaggactc gttcacctgt
     5581 tgcaggtact tcttgtcgta ctgttttgtg ctataaacgg cgagtttcat aagac
//
LOCUS       DAZGTR010000214         5595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGTR010000214 DAZGTR010000000
VERSION     DAZGTR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.216"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            232..1164
                     /gene="rfaD"
                     /locus_tag="ACHLCY_004743"
     CDS             232..1164
                     /gene="rfaD"
                     /locus_tag="ACHLCY_004743"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP0371830.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHLCY_004744"
     CDS             1174..2220
                     /gene="rfaF"
                     /locus_tag="ACHLCY_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP0371831.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHLCY_004745"
     CDS             2224..3216
                     /gene="rfaC"
                     /locus_tag="ACHLCY_004745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP0371832.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3213..4421
                     /gene="rfaL"
                     /locus_tag="ACHLCY_004746"
     CDS             3213..4421
                     /gene="rfaL"
                     /locus_tag="ACHLCY_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP0371833.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4457..>5595)
                     /locus_tag="ACHLCY_004747"
     CDS             complement(4457..>5595)
                     /locus_tag="ACHLCY_004747"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFP0371834.1"
                     /translation="DKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNP
                     GYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIV
                     VHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANAD
                     IEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAA
                     AHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLAD
                     LVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISS
                     DILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1543 a         1232 c         1263 g         1557 t
ORIGIN      
        1 cattcaaatg cttccgttat ttatcataca tcggaatatt gatactaaag cactattcac
       61 atgcaaaacc aacatccgcc atgaaggact agctaaaacc caaactagtt tgttgcaatt
      121 agcatccttg cacctctatg taaagggctg aagggattcg gatgtgatgg tatgattaca
      181 gacattcgtg tctgagattg tctctgactc cataattcga aggttacagt tatgatcatc
      241 gttaccggcg gcgcgggctt tatcggcagc aacatcgtta aagccctgaa tgataaaggc
      301 atcaccgata ttctggtggt ggacaacctg aaagacggca ccaagtttgt gaacctggtg
      361 gatctgaata tcgcagacta tatggataag gaagacttcc tgatccagat tatggctggc
      421 gaagagttcg gcgatgtcga agcgattttc cacgaaggcg cgtgctcttc caccaccgag
      481 tgggacggca agtatatgat ggataacaac tatcaatact ccaaggagct gctgcactac
      541 tgcctggagc gtgaaatccc gttcctgtac gcttcttccg cagccaccta cggcggacgc
      601 acctccgact ttattgaatc ccgcgagtac gaaaaaccgt tgaacgtcta cggttactca
      661 aaattcctgt ttgatgaata tgttcgccaa atcctgccgg aagcgaactc gcagattgtt
      721 ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc ataaaggcag catggcgagc
      781 gtcgctttcc atctcaacac ccagcttaat aacggtgaat caccgaagct gttcgaaggt
      841 agcgagaact ttaaacgcga cttcgtctat gttggcgatg tggcagatgt aaacctgtgg
      901 ttcctggaaa atggcgtttc cggcatcttc aacctcggca ccggtcgtgc ggaatccttc
      961 caggctgtag ctgatgctac gctggcttat cacaagaaag gccagatcga atacattccg
     1021 ttcccggata agctgaaagg ccgctaccag gcgttcactc aggcagatct gacaaatctg
     1081 cgcgcggcgg gttacgacaa accgttcaaa accgttgctg aaggtgtaac ggaatacatg
     1141 gcctggctga atcgcgacgc ataagagctc tgcatgaaaa tactggtgat cggcccgtct
     1201 tgggttggcg acatgatgat gtcgcaaagt ctctatcgca cgctccaggc gcgctatccc
     1261 caggcgataa tcgatgtgat ggcaccggca tggtgccgtc cattattatc gcggatgccg
     1321 gaagttaacg aagctatccc tatgcctctc ggtcacggag cgctggaaat cggcgaacgc
     1381 cgcaaactgg gtcatagcct gcgtgaaaag cgctacgacc gcgcctacgt cttacctaac
     1441 tccttcaaat ccgcattagt gcctttcttc gcgggtattc ctcatcgcac cggctggcgc
     1501 ggcgagatgc gctacggttt actcaacgat gtacgcgtgc tcgataaaga agcctggccg
     1561 ctaatggtgg aacgctatat agcgctggcc tatgacaaag gcattatgcg cacagcacaa
     1621 gatctgccgc agccattgtt atggccgcag ttgcaggtga gcgaaggtga aaaatcatat
     1681 acctgtaatc aattttcgct ttcatcagaa cgtccgatga ttggtttttg cccgggtgcg
     1741 gagtttggtc cggcaaaacg ctggccacac taccactatg cggagctggc aaagcagctg
     1801 attgatgaag gttatcaggt ggttctgttt ggctcggcga aagatcatga agcaggcaat
     1861 gagattcttg ccgctttaaa taccgagcag caggcatggt gtcggaacct ggcgggggaa
     1921 acacagcttg atcaagcggt tatcctgatt gcagcctgta aagccattgt cactaacgat
     1981 tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc tggttgccct gtatggtcca
     2041 agtagcccgg acttcacacc gccgctatcc cataaagcgc gcgtgatccg tctgattacc
     2101 ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt atcaccagag cttgatcgac
     2161 attactcccc agcgcgtact ggaagaactc aacgcgctat tgttacaaga ggaagcctga
     2221 cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg atgttctcca tacgttgccc
     2281 gcactcactg atgcccagca ggcaatccca gggattaagt ttgactgggt ggtggaagaa
     2341 gggttcgcac agattccttc ctggcacgct gccgttgagc gagttattcc tgtggcaata
     2401 cgtcgctggc gtaaagcctg gttctcggcc cccataaaag ctgaacgcaa agcgtttcgt
     2461 gaagcgctac aagcagagaa ctatgacgca gttatcgacg ctcaggggct ggtaaaaagc
     2521 gcggcactgg tgacacgtct ggcgcatggc gtaaagcatg gattggactg gcaaaccgct
     2581 cgcgaacctt tagccagcct gttttacaat tgtaagcatc atattgcaaa acagcagcac
     2641 gccgtagaac gcacccgcga actgtttgcc aaaagtttgg gctatagcaa accgcaaacc
     2701 cagggcgatt atgctatcgc acagcatttt ctgacgaacc tgcctacaga tgctggcgaa
     2761 tatgccgtat ttcttcatgc gacgacccgt gatgataaac actggccgga agaacactgg
     2821 cgagaattga ttggtttact ggctgattca ggaatacgga ttaaacttcc gtggggcgcg
     2881 ccgcatgagg aagaacgggc gaaacgactg gcggaaggat ttgcttatgt tgaagtattg
     2941 ccgaagatga gtctggaagg cgttgcccgc gtgctggccg gggctaaatt tgtagtgtcg
     3001 gtggatacgg ggttaagcca tttaacggcg gcactggata gacccaatat cacggtttat
     3061 ggaccaaccg atccgggatt aattggtggg tatgggaaga atcaagtaga gtgtcgttct
     3121 acaagtatgt ctcttgcaga tttgccagct caaaccgttt ttcaaaattt aaatcttgaa
     3181 ataataacca ataagttgac atcggagata agatgacctc aacattattt ttctctctcg
     3241 agaaaaaaaa ctggatagcg tactggaaca gagctctcgt attcttattc attaccacct
     3301 attttttggg tgggattaca aggtataaac atcttattgt tattcttatg acaataacga
     3361 caatcgtcta tctctgcaaa cggccaaaac actatctctc actatttaaa acatttcttt
     3421 ttggtagtgt tgccatatta actattgctg cattgctgtc acttcttcaa tcccctgatg
     3481 caggtgctag catgaaggaa attttcaaag ctattattga gaatacttta ctatgcacaa
     3541 tagcaatacc gatcatattg agagacgaga aaagagaaga tgtcgaaaaa atcgttttct
     3601 tctcatttat tagtgcgttg ggcttacgct gtttttctga attgattacc tattataagg
     3661 actatcaaca agggataatg ccattcgcag attatagaca ccgtagcatt tctgactcga
     3721 tggtcttttt attccctgca ttgttaaatc tctggcttat caaatcagca aaataccgca
     3781 tttcttttgt ggttctaagc gttattttta tttttctgat attaggaact ttatccagag
     3841 gggcctggct ttccgtgtta gtcattggat taatatggat tctgatgttt aaacaatgga
     3901 agttactatt agtaggagta atggttgcca tcattgcatt gtcggttatt ttcacacata
     3961 aggagatgac cgcaaagcta acgtataaac ttcaacaaac taatagttct tatcgctatg
     4021 caaatggtac tcaaggcagc gcactcgatc taatattaga aaaccctgtt attggttatg
     4081 gttacggtaa cgttgcatat aaagatgtct ataataaacg tgtcattgat tatccagaat
     4141 ggacctttag acaatcaata gggccacata attttgcgct attcatctgg tttggcactg
     4201 gtttattagg gctggtaagt cttatgatgc tatactgtgc aatattgaaa gagtgtataa
     4261 aaaatggcgt caagaataaa tatcgctcac catataatgc atattatata atcttactat
     4321 cttttatagg ttattttgtt atccgtggaa acgtagaaca aattgaacca aatttattag
     4381 gcgtttacgc cggcttatta ttagcgatga aaaacaagta aaaaataaaa aaggctgcat
     4441 aatgcggcct ttttatttat tcaaaccaat tatgaataac ctcttcgaat ctttgagtta
     4501 cgccatccca actgtagtgt tcaaacacaa agtcctgacc ttgctttgca actgctgtta
     4561 attcaggatt cgctaacgtt tttaaaatat cgctgcttat ggagtctgca gtcataggtt
     4621 cttttaaatg gaatcccgta gtattttctt tgacaaattc cgtcatcccc ccacgcgtac
     4681 tcactaaaac aggcttacca gcacccatag cttcaattgc caccatgcaa aatggttcct
     4741 ggaactggga aggaatgacg actaaatctg ccagtgggta ataacaatgc atcttttcag
     4801 gcggtatgct tcctaacata atacaacgat ccttcaaacg ttttgcaatt tccctgacat
     4861 tgcgttgata tgcacctttg tcacttttac tcatttctgt ataatcacca atcacaacta
     4921 attttaaatt tttgtgagca gcggccaatt tttcgaatga ttgcatcaac agcaatatgc
     4981 ctttgtcagg gacgatacgc ccagcataaa aaataatttt ctcttcaggg gagatattaa
     5041 gttcgcttct agtgatcggc tgaaaattag attgataggt ttctaaatca attccatttg
     5101 gtacaatctc aatatctgca tttgctaaat aagattggta atactttttc aagtataaac
     5161 taggcactat cattttaaca ttttgctcaa gcaattttgg ttcaaatgca ttatgcatat
     5221 gtataaccac tcttgcctga ggagctcgct tacgaatttg agtgtatagc ttcatgctat
     5281 tatgaactac aataacactt tctttcgtta taggaaaatc atgtgcaata ttcaatattc
     5341 tctgtgaata tggaagagga tcaagtcttg tccatttttg aaataatcgc ttatatatgc
     5401 gactgaatcc aaccctatga atactacaat tatcattaac aaaggtgtaa tttgagtatc
     5461 ctggattttt gatacaaaca atacgatttg gaaaattggt acgctgagca acctgataca
     5521 tccacgtttc aacagcagcc gctccgcgag gaggaattga aaaaatagga gtaaccgtaa
     5581 atattatttt atcaa
//
LOCUS       DAZGTR010000215         5534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGTR010000215 DAZGTR010000000
VERSION     DAZGTR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.217"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..216)
                     /locus_tag="ACHLCY_004748"
     CDS             complement(<1..216)
                     /locus_tag="ACHLCY_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFP0371835.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVY"
     gene            complement(304..2328)
                     /locus_tag="ACHLCY_004749"
     CDS             complement(304..2328)
                     /locus_tag="ACHLCY_004749"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP0371836.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGGPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(2273..3775)
                     /locus_tag="ACHLCY_004750"
     CDS             complement(2273..3775)
                     /locus_tag="ACHLCY_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0371837.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(3775..3987)
                     /locus_tag="ACHLCY_004751"
     CDS             complement(3775..3987)
                     /locus_tag="ACHLCY_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371838.1"
                     /translation="MSTEEAREMIQRYREAEIAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(3984..>5534)
                     /locus_tag="ACHLCY_004752"
     CDS             complement(3984..>5534)
                     /locus_tag="ACHLCY_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP0371839.1"
                     /translation="FTLASKRTTTFMSSGMTLVESSPGRDITDTKWRCGGAHEAPPTT
                     GILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDFVAAGQAARLMCPHCRGL
                     IAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFWMEGPAAAFQTWEQLIFK
                     LLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSELLEQRAEPFPRRSVPDG
                     VRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRHSLRCSPNGESLPVDPAA
                     YPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAGVTDNAYRFWRRCRSDGL
                     GNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVALWLVQTDAFKDRVNNALW
                     RDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGANEAFDLLVYADALAVLH
                     GYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEKRRRRKKKLRTERAEDNP
                     WITSGGWL"
BASE COUNT         1169 a         1752 c         1334 g         1279 t
ORIGIN      
        1 ataaaccacc gcgccggact gaatgtcgtc agcagactgt ttgggcagca ggaacacgcc
       61 ttcggcaaaa ccgtcaccgg cctcaccggc aggaatatcg gtaatggcca cggccaccat
      121 actgccgacc accaccgcag caccgctcag gatggtctga tctccggcat tcaccagttc
      181 aatggtggta ccgtcctgta caaaattttt cgccataatg ctgtttctcc ggacagcccc
      241 tgtggggctg tttttcaggc ataaaaaaag ccctttcggg cagtgattgt gataacgcgg
      301 ttatcaggcc accgacgaac gcaccagccc gcgccagtca agtggtgcca ctccggcatc
      361 aatacggatt tttgtggcaa tgccgtcagt ggtgaaacct tcctgctgat caatgtatgg
      421 cgtgtccaca ccatccagcc aggccacttc aatggtgtca gtgccctgtg ccgccgccag
      481 ataccaggtt ttcgggtctg ccgcatcaag acgcgcttct gcaatcacct cagcaaagtt
      541 ctggataggg ttaatgacac cggcgtttgc atccgcccct ttcacactgg ccgatttgat
      601 ggtctggttc gccaccgtct ccagtgccac cggtaccagc ataaaggccg gacggatatt
      661 cagggcacga tcgccttctt tctgcaggcg catcatctga cgggccgcat ccagtccgga
      721 aacggagatc cccccggtgg caatattttt gtgatcggca tggaacagcg ccttaccgtc
      781 ggacagtttc gggttatccg tcagcacctt gtagaccagg tcaccaatcg ttgccttcgc
      841 cgcacgcccc atcttcatcg gcacgtccac cagcatattc agatcatcat tgataatggc
      901 ctggcgggtg atggagaaaa tctccccgta agtggccagt gcaatggtct ccttgcgatc
      961 tgaggtggtg atgtatttat actccgcccc ctcacgaacc tggcgcagag aaccaaaacc
     1021 gcccatcccc acgcgatacg ctgttttgaa gtctgacagg cgtcccttac gggtccactt
     1081 ctggaaggtt tcttctgatt cctcccagcc ctggatcagc cccttgttcg acacatccag
     1141 cagaatattg ccaaaatcag aggtgctgtg cgtcagcgcc agcccgacca tctgcatggg
     1201 gttataactg gccaccccaa taccgcgctc cgtcagtgac atgcgagccc attcacgcag
     1261 ggtcatcccg ttataggcgt tatccttctc gacattttca aatccggcac gggccagcat
     1321 cgcctggcgg atcccgtccc ccacaaaatt gccgtttccg gcataaatat gggccggtgt
     1381 atttttgttg gtcggcgagg actccttgcc catttcattc agcagacgtt cacgggccat
     1441 ttccagcgaa cagtcaggat cagccacgca ctgtgcctga agcgtctgat agcgaccgcc
     1501 gaacatggca aacagatcgt taatgcctga catgcgggct ttctgctcag ccataacgcg
     1561 ggcgcgaatg gtcgcctcat cagacactgc cggtaccggt gatggttctg ttaccgccgg
     1621 tgcagggatt gtcactgtgg tatcacgcgg ggcactgttg cgtggcggag taatcatgtt
     1681 tcggatggat tccggcatct ttttaaattc ctctgtacgt tttgactgaa tacatgccat
     1741 tgcctcaaca gcgggtgtca cctggtcagc aaatccgtgt gccagacatt cggcaccgga
     1801 catccaggtt tcatccgcca gcatggcggc aatttcatcg gtggtttttc cggttttctg
     1861 cgcataggct ggcaacagta ccgattcgac tttatccagc aaatcggcat aactgcgcat
     1921 atcctcagca tccccgccac tgaatcccca tggcttatgg atcatcatga aggcattttc
     1981 cggcataatg accgtatcac cggccatcgc aatcacagat gccatcgagg cggcaacgcc
     2041 atccacatac acggtaatgg tcgccccctg atttttcagg gcattaaaaa tggcgatgcc
     2101 ttcaaagaca tcgccacccg gtgaattgat atggagatta atgtgggtga tatcacccag
     2161 tgcattcagt tcgctgacaa actgcttcgc ggtaactccc cagaaaccaa tctcgtcata
     2221 aatataaata tccgcgtcac ccggcccccc agcttgcatc ctgaaccagg atttattctt
     2281 catgctggct ttcggtgtcg cgctgatact gtcgttcagt tccggcactg ttgcctcctt
     2341 tgtcgttgac ggggtcagta tcaaagacca gccccagtct gctgttttca tcaatttcag
     2401 ccttgcggcg acgtttgacc tcatccggat tgcgcccacc ggcacgcacc cagtcagatt
     2461 ctgtcgctgc accaccccgg atctgaattc tccaggcttc agcttcctta accgggtcga
     2521 tccacggcat caccggaccg gaatacgtcg cgttatatag cgttttcatc tccacatccg
     2581 ccggaatttt cagcagacct gccgcaacca ccatattcag ccatgtccgg tacaccgggc
     2641 gggttaccgc gccaataaaa cagtcctgca ggatcaggta accatccgtg gactcgacca
     2701 gctcctgccg ctgggcgctg taggtgccgt tatagttacg cgccgcactg gaaaaactca
     2761 gacgactgcc tgctgccact gcacgcaact ggccgttgcg gaaagtttca aggttgggat
     2821 tgggacggtc agatttgacc atgccgatat cctcgccctt gcgcaaatcg tcataaataa
     2881 tacccggggt gatatggact tcccgctcgg tctctttgat ccccggatct tcatagtcct
     2941 gtccgtcacc tttacggata tacagtccca gcgccgcagc aatacgcgcc gctgtcagtt
     3001 ccgcatcctc atactcctta agggcactga tccgcatcag cacccccgat aacatggatg
     3061 agcctcgcgt ctgatgcaga cgacgagtga acttcaggtg gatcattttt ccggcagcga
     3121 tttctttcgt atcactctgc cggccgctga ccggataatt tttataaacc agatattttt
     3181 tcggtcttcc ccactcatca agaaaaaccc cctgattcag tccggcggat tcatcagtgc
     3241 gcatgggaac aaaatccggc tccatcgcct caagccagaa tggcactccc gccgtccgtt
     3301 ccagaccgtt tcccgcacca ctgaccatct gcgcaaacac ttcaccatcc cgcagccagg
     3361 tccgcagcag taaacgttca agcacaggac gggtatactg ccctgtcaca tccggactca
     3421 cggaccattc agcccacaaa cggcggatat ccgcagccag ctcagccgcc atttccccgt
     3481 tttttcgtaa tggctgaggc tccacaataa ttcccctggc accaatcacc cgctcttcca
     3541 gcttgtcaaa cacaccaatc accaggtcat gattgatatc cagaaaacgg gcctgctccc
     3601 gcagggaaac cgcaccgtat ttactgagct gatcagcaga gcgattttcc cgccgggctt
     3661 tatgtgtccg ggtcggtttc accgcctcat aggccatgat taacgccctt gaacgcagtc
     3721 tggctgcttt ccacccgggg gaaaacacgc cgatcacatc atcaataatt gccattaaaa
     3781 cctcgccagt ttaaatcccg gttttccccg cctgcggctc accatcgcgg caagcctgcg
     3841 ttcccactcc tgacgtccgg cgcggatctg agaaaggctt tccagcgtca gttgctgccc
     3901 gttgaaggtg acagactttc cctccagtac ggctatttcc gcttcacggt accgctgtat
     3961 catttctctg gcttcttctg tgctcacaac cagcctcctg atgttatcca tggattatct
     4021 tccgcacgct ccgtccgcag ttttttcttc cggcgacggc gtttttctgc cccggccgtc
     4081 agttccgggg ataccgtttc accagaacgc tcctgcggga agacgagcca cgtttcccgc
     4141 tgtgcccagt ccggtgcgga gggccagcgg atcttttcgt aaccatgcag aacggcaagc
     4201 gcatccgcat aaaccagcag gtcaaacgct tcgttagcgc ccctgcccgg ttttcgccat
     4261 tttccgtcac tgccgcgctc ttcataggtc agctcatcgt aaaaccaccg ccccagccag
     4321 tcgggaaagt ggatatagtt cggccctggt gtgtcacgcc acagggcatt atttacacga
     4381 tccttaaacg catccgtctg aaccagccac agcgcgacat cgccactggc tctggcacgg
     4441 cgggcacttc tgccggtatt atccgggaag gttcggttaa tcagcctgtc acggcgaagt
     4501 ccatccccct tgaacagaaa caccctgttg cccagtccgt cactccggca acgacgccag
     4561 aaacgatagg cgttatctgt caccccggct tcccctcccg tatccaccgc catggccatc
     4621 agacgcatgc gcacatccgg atcagaagcc agcggccatg ttttatggaa cacatccgtc
     4681 agcaacaaat cccagtcctc cggatatgcc gccggatcaa ccggcagact ttcaccgttg
     4741 ggactgcagc gcagtgaatg ccggatgttg tagcgatcaa caatccagcg ttccccctgc
     4801 tctccgtatc cggtgatctg cacaacaaaa cggcgatttt taccgccctg tacgtcaacc
     4861 gttgcctcaa taaaacgcac accatccggc acagatcgcc ggggaaacgg ctcggcacgc
     4921 tgttcaagca gttcactttt acgctgttcc gtggctgaac ggggcagata gggtcgtccg
     4981 atatcggtgt tcaccaccgc tttcagggtc tcttcactgc cggttcgctc atactcttct
     5041 tctgccgcca gcagtttaaa aatcagttgt tcccaggtct gaaacgccgc agctggcccc
     5101 tccatccaaa atgacgcaat ccgggagttt cgtggcgttc cggtgatact gccgtccgcc
     5161 gccgcccgtt caccttcacg aagccagatc ccctggttat tcagttcgcg tttctgctca
     5221 ggggcaatca gcccgcgaca atgcggacac atcagacggg cagcctgacc ggcagccaca
     5281 aaatccgggt tattccggta tccggtcatg ttatccatca ccggctgaaa atattccccg
     5341 cagtgcggac acggccagta ccaccggcgg cggtctcccc ggttatacag tgacaggatc
     5401 cccgttgttg gcggtgcctc atgtgcgcca ccacaacgcc atttggtatc ggtgatatcc
     5461 cgccccggtg aactctcgac cagggtcatc cccgaggaca taaaggtggt ggtacgcttt
     5521 gaggccagcg tgaa
//
LOCUS       DAZGTR010000216         5338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGTR010000216 DAZGTR010000000
VERSION     DAZGTR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.218"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..283)
                     /locus_tag="ACHLCY_004753"
     CDS             complement(<1..283)
                     /locus_tag="ACHLCY_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0371840.1"
                     /translation="MADFDNLFDAAIACADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRIS"
     gene            complement(295..690)
                     /locus_tag="ACHLCY_004754"
     CDS             complement(295..690)
                     /locus_tag="ACHLCY_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0371841.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVTDTADLVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(732..1757)
                     /locus_tag="ACHLCY_004755"
     CDS             complement(732..1757)
                     /locus_tag="ACHLCY_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0371842.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSVAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            complement(1813..2145)
                     /locus_tag="ACHLCY_004756"
     CDS             complement(1813..2145)
                     /locus_tag="ACHLCY_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0371843.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(2155..2733)
                     /locus_tag="ACHLCY_004757"
                     /pseudo
     CDS             complement(2155..2733)
                     /locus_tag="ACHLCY_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
     gene            2740..3021
                     /locus_tag="ACHLCY_004758"
                     /pseudo
     CDS             2740..3021
                     /locus_tag="ACHLCY_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(3037..3113)
                     /locus_tag="ACHLCY_004759"
     tRNA            complement(3037..3113)
                     /locus_tag="ACHLCY_004759"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3077..3079),aa:Arg,seq:tct)
     gene            3356..3988
                     /gene="fimZ"
                     /locus_tag="ACHLCY_004760"
     CDS             3356..3988
                     /gene="fimZ"
                     /locus_tag="ACHLCY_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP0371844.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(3991..4506)
                     /gene="sfmF"
                     /locus_tag="ACHLCY_004761"
     CDS             complement(3991..4506)
                     /gene="sfmF"
                     /locus_tag="ACHLCY_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP0371845.1"
                     /translation="MRRVLFSCFCGLLWSSIGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(4517..5338)
                     /gene="fimH"
                     /locus_tag="ACHLCY_004762"
                     /pseudo
     CDS             complement(4517..5338)
                     /gene="fimH"
                     /locus_tag="ACHLCY_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
BASE COUNT         1306 a         1437 c         1309 g         1286 t
ORIGIN      
        1 gagaaatccg gtctatccag aagttttcct caccgatggt cagcgtgtcg ccgcgccgca
       61 gctgccgcac atcatcagtc cggacaaaca gggacgggct ggagccttca acgcgcacgc
      121 cctgtccggc atagctgata ttttcagggt catcaaaaac accacgtatc acagcaccgg
      181 actgctcacc ggatgtcatg gtggctgacg ttcccatgta cccgcgtatc gtttcatcgg
      241 cgcaggcaat ggcagcatcg aacaggttat cgaaatcagc cacagcgcct cccgttattg
      301 cattctggcc aggccgcgct ctgtcatttc agctgccaca ccggcagaga cacggaacgc
      361 cgttcccggc agcacaaatg ccacagcctc atcccgcgtg gcgtgaagtg catcagtatg
      421 cagcgtcacc agtgccacaa ccgtgaccag atcagccgta tcagtcacgg tatccggctg
      481 cgctgataca acctcatttt catgtccggt cagcgcattt tccgggctga cagatgtgtc
      541 ctgaccggca gcgtcatccg tgtcatcaag ctcctcttcc agctctgcca cacggagtgc
      601 cagttcttct ttcgtccccg tcaggctgac atcacggttc agttgctcac ccagcacctg
      661 aagacgggca atcagttcat ctttcgtcat ggactcctcc acagagagaa aatggccccg
      721 aagggccatg attacgccag ttgtacggac acgaacgcat cagggtcagc cagcagcatc
      781 agcggtgctg actgaatcat ggtgaactca cgcgccggat cgccggtggt cacccagttt
      841 ttcgggtagc gggcagaggc gttaatacct tcgcgctgtg cgtccgcatc ctgaatgcag
      901 ccataggtgc gcagaccgcg tgcctgagtg ttccccagca ccatcgtgtt gtccggaagg
      961 aagttctttt tgacgtcgtt ttccacgtac tgtccggaat acacgacgat cgccgtatcg
     1021 ccatacatcc ccttatagga caccgcttcg cccaggtctt ttaccgctgt ctccagctcg
     1081 gaatgagagc cgcgacgggt atccagcttc tccctgacgg ctttgaagga acggaacagc
     1141 gcccagcctt tcggatcaaa cacgatgata ttcaccacac cgctggcgtt cagcgcgtag
     1201 gcttcgatat cgtcggtcgg gtcatacgtg gacttgtcac gcttgctcca ctccgtaccg
     1261 ccggactgcg tgatgttgtt cgccacactg cggcccatat ccacttcaac cggatcgaag
     1321 gcttcaccgg tcatggtgta tttgccctta agcacggcag aaactgcctg catctcttcg
     1381 acctgagcaa tggccagctc ttcgtctcgc atgttctgca ggatgatgcg acggcggcgg
     1441 taagccgggt ccgccagatt ctgtggatct tcatccggca ggcgacgcag ggtcatctgc
     1501 ggattcactt catgcttggg tttgacatat cccggcgtaa attcagaggt ggagccgcca
     1561 cgggagcgga taacctcacc ggaaacaatc ggcgaaacgt acagcgccat gtttaccagt
     1621 cccggaattt gtgagagata gactttctcc gtggtgaagg gatagctctc acggaaaaag
     1681 agacgcagaa acagcggatc aaacttaaat ttctgctcat ttgccgccag cagctgggcg
     1741 gttgtgtaca tcgacataaa aaaatcccgt aaaaaaagcc gcacaggcgg cctttagtga
     1801 tgaagggtaa ggttaaacga tgctgattgc cgttccggca aacgcggtcc gttttttcgt
     1861 ctcatcgctg gcagcctccg gccagagcac atcctcataa cggaacgtgc cggacttgta
     1921 gaacgtcagc gtggtgctgg tctggtcagc agcaaccgcc agaatgccaa cggcagcacc
     1981 gtcggtggtg ccatcccacg caaccagctt acgggtggcg gtgtccagca tcagcggggt
     2041 cattgcaggc gctttcgcac tcaatccgcc gggcgcggtt gcggtatgag ccgggtcact
     2101 gttgcccagc ggctggtaat gggtaaaggt ttctttgctc gtcataaaca tcccttacac
     2161 tggtgtgttc agcaaatcgt taacggcatc agatgccggg ttacctgcag ccagcggtgc
     2221 cggtgccccc tgcatcagac gatccagcgc agtatcactg cgcgcctgtg cactctgtgg
     2281 tgctgcggcc agaatacggc gggccgtttc cacggtcata ccgggggttt ctgccagcac
     2341 gcgggcctgt tcttcgcgtc cgtgagcctc ctcacagttg aggatcccca taatgcggct
     2401 gttttctgcc gcaaccgcag cggtgatctg cgcgttcacg tccggctgcg ccgcgctggc
     2461 gttttcgccc tccatcgctg gcaccacgcc agtaacgtca gcctgcgaag cagtggctga
     2521 aacagttgtt gattgagtct ctttggtcat tcgccctcct gagagacggg atttacgtgc
     2581 atccagtgca tcacgcataa cggtgatcgc atcggtgctg ttgacaagtt catcagccag
     2641 tccggcatca atggcctcct gaccgctgta cactgcagcc tcggtatcca gcacagcctg
     2701 cacggacagg ccggtatatg ccgacacctt ctgcggttca gcaagacatc atggctatca
     2761 gcttgtcgtc gtgcaggaat tgaagatttc cgtttccatg acctcagaca cacctgggca
     2821 agctggctga ttcagtcagg cgtcccatta tcagtgcttc aggaaatggg cggatgggag
     2881 tccatagaaa tggttcgtag gtatgctcac cttgcgccta atcatttgac agagcatgcg
     2941 aggaaaatag acgacatttt tggtgataat gtcccaaata tgtcccactc tggaattatg
     3001 gaggatataa agaaggcgta actgattgaa ttgtaatggc gcgccctgca ggattcgaac
     3061 ctgcggccca cgacttagaa ggtcgttgct ctatccaact gagctaaggg cgcgttgata
     3121 ccgcaatgcg gtgtaatcga gtgaattata cggtcaaccc ttgctgagtc aatggctttt
     3181 gatctggttg ctgaacaagt gaacgaccgc gtctgatttt ctgatttatt tcgctatagc
     3241 ggcaaacaaa cacacaccgc tgcgcgtctg aatcaagaaa acccgtattt tcatgtatca
     3301 aagtacaatt taccgcccta acggaaaatt gtccgctcct atgagactgg taactatgaa
     3361 accaacgtcg gtgatcatta tggatactca tcctatcatc agaatgtcta ttgaagttct
     3421 gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg gatgattacc gcataaccat
     3481 cgattatctc cgaacccgtc ctgttgattt aatcattatg gatatagact tgcccggaac
     3541 agacggtttt accttcctga aaaggatcaa acaaatccag agcacagtga aagtgttatt
     3601 tttatcatcg aaatcagaat gcttttatgc tggcagagcg atacaagctg gtgctaacgg
     3661 ttttgtcagt aaatgcaatg atcagaatga tatttttcat gccgttcaga tgatcctctc
     3721 cggatacacg ttttttccca gcgaaacgct taactatata aaaagcaata aatgtagtac
     3781 gaatagttca acggtcactg tgctatctaa tcgtgaagtg accatattac gttatctggt
     3841 tagcggatta tctaataaag aaattgccga taagttatta cttagcaata aaacagttag
     3901 tgcgcataaa tctaatattt atggcaagct aggtttgcat tcaattgtag agcttatcga
     3961 ctacgccaaa ttatacgaat taatataata ttaattataa ttgatcataa atatcgcatc
     4021 cgctttcgcc acacctggcc gaacaccgct ggctaacgct cgataattgg caaaaaagtt
     4081 tagtgtgaca ttgccatttg catctacttc ctcagtcggg ctcgcctccc ccagtgcgag
     4141 ccgggagcga tcgctattac gtaattcgat ggcgacggtt tgtgccattg cgggatcatc
     4201 cagagccagc aggttggtat cggatgccgg cgttcccgta aataaaatcg caactgaacc
     4261 cggaggacat ccctccagcc gcaggctaaa agggacgagt gccgtggtat cgccagcgtt
     4321 cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc ttatcaatat ccgctgtgtt
     4381 tacggtacag gagaaatcaa caacgttacc gtgcaaattg atattaatcg ttcctaaagg
     4441 gtcagctgcc catccaatgg aactccacag taacccgcag aaacagctaa agagtactct
     4501 tctcattctc cgtacctcat tgataatcga cgcgtaaata cccctgcgcg ctaaacggcc
     4561 cctcggtcgg tttttgaccg gtaatactga taggccaggc gcgaagtgtg aaccgccccg
     4621 ggtttcctgg agacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg
     4681 agatcgttag gcgtaatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga
     4741 ttgtccgaca ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa
     4801 gcctgcgcag caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacgcca
     4861 gctggtcgat tccccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca
     4921 aattcaacaa tctgcccggc atttaattcg cagttttgcg gcacttcaac ccggccacca
     4981 aaactaatgg tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg
     5041 gtaaacatca cctgtctggg gatctccacc atgttaataa agggacgaat aacctttaaa
     5101 cgaaatatta aacgagaatc catcaccccg aatggaaggt tttgcgaaac gttaggatct
     5161 gttcccatcc gaatataatt ttgcgggggg aactgaatat ccatcacact atcaaccaga
     5221 ctcatcgcac ctaatagata atcatgtaat tgcatatatt tataattatc gatagtttcc
     5281 tgaacaataa agttggtgac ataactacgg tatgtataat tcaccagcgt accgggtg
//
LOCUS       DAZGTR010000217         5297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGTR010000217 DAZGTR010000000
VERSION     DAZGTR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.219"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(243..455)
                     /locus_tag="ACHLCY_004763"
     CDS             complement(243..455)
                     /locus_tag="ACHLCY_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371846.1"
                     /translation="MDIEMNNTEEMFSKLGMRLGAEMAIPLKDGDNFAPTGCIVTKLD
                     KENGLLICRPVQKEINGTWYTVDLSK"
     gene            609..833
                     /locus_tag="ACHLCY_004764"
     CDS             609..833
                     /locus_tag="ACHLCY_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="HFP0371847.1"
                     /translation="MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYY
                     LSAETAARVRRGDEAVHSSEDVRKSLGLDD"
     gene            817..1083
                     /locus_tag="ACHLCY_004765"
     CDS             817..1083
                     /locus_tag="ACHLCY_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0371848.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSG
                     RPLKGELGEFWRYRVGDYRILCEIRDDELIILVATIGHRREVYD"
     gene            complement(1142..1564)
                     /locus_tag="ACHLCY_004766"
     CDS             complement(1142..1564)
                     /locus_tag="ACHLCY_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP0371849.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLQST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            1686..1964
                     /locus_tag="ACHLCY_004767"
     CDS             1686..1964
                     /locus_tag="ACHLCY_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371850.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(1956..2246)
                     /locus_tag="ACHLCY_004768"
     CDS             complement(1956..2246)
                     /locus_tag="ACHLCY_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371851.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGMNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            2491..3963
                     /locus_tag="ACHLCY_004769"
     CDS             2491..3963
                     /locus_tag="ACHLCY_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFP0371852.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVG
                     GGGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQR
                     LKDIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALR
                     EAERQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELA
                     KAESDVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSV
                     TNALNSAVSIRDAKKTDVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEK
                     YSRIAQDLAEKAKGSKFSSVDEALAAFEKYKNVLDKKISKVDRDAIFNALESVNYDEL
                     SKNLTKISKSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVAL
                     MFSFIVGVPLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
     gene            complement(3960..4250)
                     /gene="cki"
                     /locus_tag="ACHLCY_004770"
     CDS             complement(3960..4250)
                     /gene="cki"
                     /locus_tag="ACHLCY_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HFP0371853.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
     gene            4280..4411
                     /locus_tag="ACHLCY_004771"
     CDS             4280..4411
                     /locus_tag="ACHLCY_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0371854.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(4606..4749)
                     /locus_tag="ACHLCY_004772"
     CDS             complement(4606..4749)
                     /locus_tag="ACHLCY_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0371855.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDT
                     QNS"
BASE COUNT         1401 a         1030 c         1345 g         1521 t
ORIGIN      
        1 cagtgtcatg aggtttacgc aggcaaaacc agcgttattc acatggctga atagctatgc
       61 atacggttaa aatttatcag gtgcgatcgc ggcagctttt cgggtggttt gttgccattt
      121 ttacctgtca gcagccgtga tcgcgctgaa cgcatttcag cggtgcgtac aattcagatg
      181 ttatgttcaa tcagagtcag cggcaggtat aaaaaagccc cgcagggctt tagtccgtgg
      241 tgttacttac ttaaatcgac ggtgtaccac gtgccgttga tttccttctg aactggtcgg
      301 cagatcagaa ggccattctc tttatcaagc ttcgtaacga tgcatccagt tggcgcaaaa
      361 ttatcaccat ccttcaaagg gattgccatc tcagctccta accgcatccc cagtttgctg
      421 aacatctctt cagtgttatt catttcaata tccatgttgt tttcctttta aaaccgatac
      481 ttatcatata tcagtcgtta atatatggga ttttactgtt tattcaacca gcctttgatt
      541 gttttatcgt ctttttacgt tataattttt gctatacatg tataacaatt gttcggaggg
      601 agatttgtat gctggctatt cggttgtctg atgaaattga gtcccgtctg gactcgctgg
      661 cgaagaaaac cgggaggaca aagacgttct atgcccggga agcgatactg gcgcacctgg
      721 aagatctgga agattattat ctttcagcgg aaacagcagc acgtgttcgc cgtggtgatg
      781 aggctgtgca ttcgtctgag gacgtaagga agtctcttgg tctggacgat taattattcc
      841 gatcgggcgc tcaaatcgct acgtaagatg gacaagcaga acgcgcgacg gattgtggat
      901 tttatggatt tacgtattgc agttgcagcc gatccccgta aatcagggag gccactgaag
      961 ggggagttgg gagaattctg gcgttatcgt gtgggtgatt atcgcattct gtgtgaaatc
     1021 agggatgacg aactgattat tcttgtggcc accatcggcc atcgacgtga agtctacgac
     1081 tgaagcccgc catgcgggct ttttcatatg tggcgtacaa taagggatta tgataaatcc
     1141 cttactgtct gccctcgtat ccatcgagat aaaccgcaga aatggtgtct gccattagtt
     1201 tcattttttt cggcgaaggc atatcgctgt ttgtgtattt tctgccagca aaggttgttg
     1261 tgctctggag tgagtgaaaa aatttttctg agtggaattc ttttacacgt tgctgtgccg
     1321 tttctgctga tactccggct gctttgtctt tcatccccat atcccggaac tgctgaaaga
     1381 acgggagcat catacgtatg gtgtctgctt tctgggtgta gaagttagga tcaaatccat
     1441 catctgaagc ataaacataa tgccgggtat gtcgttccag gctggtcttt ggtgtgcatc
     1501 ctgtaagagc tattgttgct gttatcagag gtatcagaag cagcctggca gcggcagatc
     1561 tcataaacat ctccttgcgt ttttaatctt gatactcagg gagttatatc tccgaagagt
     1621 atctcttatc atacgtcggt ttactcccaa tgtgatttga tggtcaatga gtcaggaata
     1681 atcccgtgaa aaaatgtgtg ccagtcctgc tttttagtgt tctttctgct tcggttgcca
     1741 gtcatgcgga cagcaatgtt tttcaacaaa aggccgagaa gacaaaaaac ggcattgttg
     1801 cggatgttaa tgccagacag aaaatgttca gtaacagact gaagatacaa cttcagatcg
     1861 atgagttgag agcagagctg gctggcacca gagatgccca aaaaagagag gatttgcgta
     1921 aaaaactgga gtctctggag cggcaaaaga aatccttacg ctgatggggg cgtattttcc
     1981 ccggtttctt ccataacctt tttaccgttc agatatttac gggcgaggcg aatgcgtctt
     2041 gcctggtata ccgccgagta agcctgggtg ggattcatac cattactgat ttcactgacc
     2101 acatgacgct gatctgccag tgtcagcgtg taaacctgtt tctggatgag gtcacgggct
     2161 ttgtcatgct catatccggc agcaacaagg gtgtcgagaa gtcgtttgtc tgttttgttc
     2221 agtttatggc ggggtgattt gctcacatcg ttctccgttt tttcatgatc caacagtttt
     2281 atatctcatc ctgatttgct ccggtgttct gtttcgacgt tctcaatgcc tgcctcccca
     2341 tagtgtgatc cttattgcaa aaatgaaaat ccatgctctt gacatggaca atgctgagta
     2401 gtaggttttc actgtacata aaaccagtgg ttatatgtac agtatattgt ttttaattta
     2461 ttgttttaat tatcaaagag gaatttcttt atggataaag tcactgataa ttctccagat
     2521 gtggagagca cagaatctac tgaggggtca ttcccaactg ttggggttga tactggcgat
     2581 acgattacag cgacgcttgc aactggaact gaaaatgttg gtggaggcgg tggagcattt
     2641 ggtggggcca gtgaaagttc tgctgcgata catgcaaccg ctaaatggtc taccgcgcag
     2701 ttgaaaaaac atcaggctga acaggctgcc cgtgctgctg cggctgaggc agcattggca
     2761 aaagcgaaat ctcagcgtga tgccctgact caacgtctca aggatattgt taatgacgct
     2821 ttacgtgcta atgccgcccg tagtccatca gtaactgacc ttgctcatgc caataatatg
     2881 gcaatgcagg cagaggctga gcgtttgcgc cttgcgaagg cagagcaaaa agcccgtgaa
     2941 gaagctgaag cagcagaaaa agcgctccgg gaagcagaac gccaacgtga tgagattgcc
     3001 cgccaacagg ctgaaaccgc gcatttgtta gcaatggcgg aggcagcaga ggctgagaaa
     3061 aatcgacagg attctcttga tgaagagcat cgggctgtgg aagtggcaga gaagaagctg
     3121 gctgaggcta aagctgaact ggcgaaggcc gaaagcgatg tacagagtaa gcaagcgatt
     3181 gtttccagag ttgcagggga gcttgaaaac gctcaaaaaa gtgttgatgt gaaggttact
     3241 ggatttcctg gatggcgtga tgttcagaaa aaactggaga gacaattgca ggataagaag
     3301 aatgaatatt cgtcagtgac gaatgctctt aattctgcgg ttagcattag agatgctaaa
     3361 aaaacagatg ttcagaatgc tgagataaaa ttaaaagaag ctaaggatgc tcttgagaag
     3421 agtcaggtaa aagactctgt tgatactatg gttgggtttt atcaatatat aaccgaacaa
     3481 tatggggaaa aatattccag aatagctcag gatttagctg aaaaggcgaa gggtagtaaa
     3541 tttagtagtg ttgatgaagc tcttgctgca tttgaaaagt ataaaaatgt actggataag
     3601 aaaatcagta aggttgatcg ggatgctatt tttaatgcgt tagagtctgt taattatgat
     3661 gagttatcta agaatttaac gaagatatca aagtcgctta aaatcactag tcgagtatct
     3721 tttctatatg atgttggttc tgattttaaa aatgcaatag aaactggtaa ctggcgacct
     3781 ttgtttgtca cgctggagaa aagtgcggtc gatgtaggtg tggcaaaaat tgtggcatta
     3841 atgtttagtt ttattgttgg tgtgcctctt ggtttctggg ggattgcaat tgtcacaggt
     3901 attgtttctt cttacatagg ggatgatgag ttgaacaaac ttaatgagtt gttagggatt
     3961 taatttctct ttagagtgta atatatgaaa cttggcaatc ctactgggat tgccattaat
     4021 aaataaaaca atgcagataa cttgcataag taccagggtt ctttttttaa ctttgtattt
     4081 tctttgatat atctagatat ggttaattta gagtatggat atagcaatac atgtattgat
     4141 gatataaata taaataacaa aggagtgtta tcattgtcgt taaatattaa aataagaatc
     4201 catggtataa gtgattcagg taaattatgt aggtagtatt ttaaatgcat aagtattttt
     4261 acttttgttg gaaagaaata tgaaaataat aatttttcta attacaatat tattcttgtc
     4321 aggatgtcaa gcaaactata ttcgtgatgt gcaaggagga accgttgcac catcttcgtc
     4381 ttctgaactg acggggatcg cggttcagta gaaaagatca aaggatcttc ttgagatcct
     4441 ttttttctgc gcgtaatctg ctgcctgtaa acgaaaaaac cgcctgggga ggcggtttag
     4501 tcgaaggtta agtcagttgg ggactgctta accgggtaac tggctttagt ggagcacaga
     4561 taccaaatgc tgtccttcca gtgtagccgc tataaggctt caacttcaag agttctgcgt
     4621 atctaaatcc actatccagt taccaatggc tgctgccagt ggcgttaagt cgtgtcttac
     4681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat accaacagcg
     4801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaat
     4861 cggcagggtc ggaacaggag agcgcacgag ggagcttccg gggggaaacg cctggtatct
     4921 ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc ggtttttgtg atgctcgtca
     4981 ggggggcgga gcctatggaa aaagcgcctg tgccgcggcc tttcccgggg tcttcgtttt
     5041 tttgcgcaca tgttctttcc cgggttatcc ccggattctg tggataacgg ggttaccgcc
     5101 tttgagaaag gagagtccgc tcgccgcagc cgaacgaccg agcggagcga gtcagtgagc
     5161 gaggaagcgg aaaagcgctt ggacgtgcat tctctcctta cgcatctgtg cggcatttca
     5221 cacccggcat ggcgcacttt tcatacaatc cgcactgttg ccgcataatt aagccagtat
     5281 acactccgct atcgcta
//
LOCUS       DAZGTR010000218         5294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGTR010000218 DAZGTR010000000
VERSION     DAZGTR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.220"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..281
                     /locus_tag="ACHLCY_004773"
     CDS             <1..281
                     /locus_tag="ACHLCY_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP0371856.1"
                     /translation="GERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISK
                     RYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            298..558
                     /locus_tag="ACHLCY_004774"
                     /pseudo
     CDS             298..558
                     /locus_tag="ACHLCY_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(700..1824)
                     /locus_tag="ACHLCY_004775"
     CDS             complement(700..1824)
                     /locus_tag="ACHLCY_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP0371857.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(2554..2751)
                     /locus_tag="ACHLCY_004776"
     CDS             complement(2554..2751)
                     /locus_tag="ACHLCY_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0371858.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(2817..3087)
                     /locus_tag="ACHLCY_004777"
                     /pseudo
     CDS             complement(2817..3087)
                     /locus_tag="ACHLCY_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3316..3588)
                     /locus_tag="ACHLCY_004778"
     CDS             complement(3316..3588)
                     /locus_tag="ACHLCY_004778"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371859.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASSSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWEAIYRSTSHYGKNVLLLQLA"
     gene            3902..4096
                     /locus_tag="ACHLCY_004779"
     CDS             3902..4096
                     /locus_tag="ACHLCY_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371860.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            4439..4567
                     /locus_tag="ACHLCY_004780"
     CDS             4439..4567
                     /locus_tag="ACHLCY_004780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371861.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(4877..5212)
                     /locus_tag="ACHLCY_004781"
     CDS             complement(4877..5212)
                     /locus_tag="ACHLCY_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371862.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
BASE COUNT         1567 a         1201 c         1115 g         1411 t
ORIGIN      
        1 tcggtgaacg cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg
       61 tcgtggtatg gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc
      121 acgttatcag caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac
      181 atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca
      241 aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc attacctgag
      301 aataatacct cctgggtaag tgagcagata ctttcagcgc ctccgaaaga tgtaaatatt
      361 cgacttggag tcggttcttt agaaggtaca accgtctcac atgttcagcg attgcatcat
      421 tcgttaatcg cagctggttt ggaaagtaac ctcactgtct ataccggtgg tcacgattat
      481 gcctggtggc gcggagcaat tattgatgaa ttagcaaatt ataattgcag gaaggtatca
      541 aataataact ctgtgtaaaa tatcgtttta tcgtcagata actctttctg caatattatt
      601 atgggagact atctcattta tttagtgatg tccttcattt atgtgatgaa ggacattata
      661 ttactcataa catttctcgt tgaaaatttc agcaatacgc tacaattgtt tcccgttgtt
      721 ttttaatttt ctcatcaaaa cctctgcaat ctctgcagga ctgggttgtt cagccatttc
      781 agcggctacc tgatctgaac agaagcgtag tgaatcatca taaagaaggc gatttaccaa
      841 atcactggtc agtccatgct tgtccggaat aatcccacac ccacgcttcg caacaatttc
      901 tgcattaaca gagcgatctg caccttcgcc aaacacaatc tgtggtattc cactatacag
      961 cgctgtcagg gtattacctg cgccaccgtg atgaataaat ccatcagctc cattaaggaa
     1021 tacacccata ggtatccagt caaccagacg tacatttgat ggtagttttc gtaaaccagt
     1081 acgagcattt attgcaagtt gcaaaatgat atcagcatca acttcatttg cagaatccat
     1141 aacccatgaa atcagctcca gaccatcaac cattggtttt acagtgccaa ggctgataag
     1201 taaacgtttc cgatcagaat ccctgtccca ccatgtttcc tttacagcac ctccgttata
     1261 aggaatatat ctcattgaga taaccggttc tccagcattt ttaagaatgc tcatacttgg
     1321 gggagcaaca tcaatccatg ctaaatcaca tagtggaccg ctgactccat gccgcctgta
     1381 agcattgctt aaagaacggt ttaacatctg aatatgggca gatgtatgcg cgaatccaac
     1441 agccagcatc actgaaggaa ttctatattt agcagcaacc aatgggcctg ccggatcaag
     1501 agggggatag actattaaat ctggcctcca ttttcctgca aaatcgatga ggttatctgc
     1561 catttcatcg ctaaaaaaag agaaatgacc aataatatta ctttttttcc tcaactcttc
     1621 ctgatggcga tatccagcct ctgaatctaa acctggaact gcatcaaaaa caaccagtcc
     1681 agcttctgct gctttattcg cgaatttgcc agcgctacta attactacat catgtccaat
     1741 tacacgaaag gcctgagcca gagaaatcaa tgggtataac aaaccgtaca gcggtgggcc
     1801 aataaaaaga atacgttttc gcattaaaaa agtccgtctt ctgcttacct gtttattttt
     1861 ctatctctaa gaaaacataa ttaatattat ttttatgtga gcttatgcac aaaaataacc
     1921 aagtatatta tcttctggta gagattcagg atacagtttg taggagcttt cttttttgaa
     1981 caatgatttg caggacagtt atgctaaact agcggaatca gtatcacaac aataaatgac
     2041 tgatcatacc tgaaatttga attaacaggc atcatgaatg tcaatagtct aagcggtgac
     2101 tacttatatc gcttgcataa atactaaaca ctgactagaa aagtattgga taaccccgtc
     2161 actaaaagta acgcttcggt tatttgctcg gaatgtatta acgccaagat tgttagccgt
     2221 tacgccttat atcactggcg ctgacaaatg tttcttttct aatttactga accggcgcag
     2281 acacgcagga aagcacgtcg gcaccggtgt aatgccagtt ctaatcaata agaatggaat
     2341 gacgaatcag tcaggaaggg ataacaaccc gctgctgaac ggaaggtatc agcaggaaga
     2401 tgaacgggaa tatcgccagc accaagggca gggccgggtt ccacaatacc agcgctggca
     2461 ttttgcacat tgccagcgta ccggcattac aacacgtttc atgctgctgc cccccaggat
     2521 tcctcgattc gttgctacat ccactgctct acttcacttc cactgctcca cttcactttt
     2581 gtaccagcgg ctgctgcgcc ccagtttgat gggtttaggg aaatggcctt cactgatcaa
     2641 cttgtaaaac catttgtcgg tcataccggt gaaactggtg atatagttca tgtcgatcag
     2701 ggaattatcg gcacgcagaa atacgctgtc gtggcttgtt gtagtcaaca tggtcagaat
     2761 gtcctgtgag atttatgaag attgtgcgaa tgcgggaaat ctactgagct gtgctttcag
     2821 aactggcctg ttacgggata ccaaggatta ccggcggggt aacgggcttc cggatcatac
     2881 acaccacgat tatcgcggac aaaatcactg aacgcccata tcacctcttt aagtatgtct
     2941 tcgcagtccg gtaaatgacg atccagcgcc acatcccggg tggtacagct ttgatgcgcc
     3001 cggtgacaca aagcccggat tgttccagac atcctgaatc aaacgcccca gattaggggc
     3061 gtcgaaatat gcctctctga ccattatatt ccggcgtata ggtagcaggt ctgaagtgac
     3121 aatgcgtcac ctgacgttaa aagtcaccca agatgacccc agtgggggca acaagtataa
     3181 aaccgttttt tgattaagtg gtcggatatg aaaaggggaa ggtttatgtg aaaaatacct
     3241 gtgttttctt tgaaaacaca caacagtatc aggttattta tgtttataca gtgcgttcca
     3301 aactgacctg aataattacg caagttgcaa caataaaaca tttttgccgt agtgtgaggt
     3361 actccggtaa attgcttccc aggtgcagac aatgactaac gaagaactac aggcaatcgc
     3421 attatccatg cttgagcgcc agcgcgaaaa agtacaccag caacgagagt ctttgcttaa
     3481 cccatctcca ttcaccaggc caaaacgatt ggagcttgaa gcattcttgg acaaataccc
     3541 acggcggctt aaatcaggca aaaccagacc accaggctga tagctcacag tttgttctcc
     3601 gattcaataa aacggataag aaccatcagc gcggtagcgt aaggcactac agcgcattgc
     3661 tttacgagta ataacaatgg atttatcacc agaagacgca aagtttttac gagaactgca
     3721 acaccgtatg ctcagcatta tcgaacagca aacgggcatg agcgccatag acccattgcg
     3781 atacaaacgg gcctcatctc ctttagttga cgacaattcc aatgcggata aaacacgtcc
     3841 accacactga ttacattttt cattagcaca gtggtgtgat gcagcatacg aggaaaaccg
     3901 gatgaatgag aacaaaataa aaaggctgga gcaactgtta caggcacgcc aacgggagtt
     3961 tgccactaaa tggggacgtg ccgcgctgcc acatgaacgc ctaacctatt ttccacttga
     4021 agacctcatt aaaaataatg gcaaaaaaat caggtctgat aagaaacact tcttagaatg
     4081 caatgaagac aaataacaac aatctgcata ttgccaccaa ccacagatgt ggcatcgtta
     4141 tcagcgcact tgattttctt tatatccact gacgagtttg ccggtcattc tggtatcatg
     4201 cagatattta cggcgtacaa gcacactagc atgacattgt aataaggcct gagcctaacg
     4261 ctttaatgtg tgtcatacac tcagtggcgt aatcgttgac aatatggtgc atgtaatcgt
     4321 aagcgtccag cgaaatccat ctggtcataa tctgaagcat ccgacaaagt ggtgtccacc
     4381 aaataagtga tctgcgccca atatcctgag ccggaacttt gacaaaggcc aacgaaaaat
     4441 gggagcggat gttacagagt tctcggtaca ggggaaaagt ggtacctgtc gccagttctc
     4501 cgatctgttt aaccgggaaa ttatctccta cagcttgtca gaaaggtcgg tgatggagca
     4561 tcgttaatac gatgctatac gatgcattca cagtgcccgg ccagaggatg ccccgtcgct
     4621 gcatatggat cagggttggc aatatcgaat tgcaggctat aggcaaagtt aaagccccat
     4681 ggagtagcac aaaatatgcc gcacaaagga aacggtctga ataacgcagt gatgaagaac
     4741 ttcttcagca cactgctaaa acgcatagtg atcgagcgct gattctggcg aacaactgaa
     4801 ctaatacatc agaatctgca ttatgttaaa taaatataaa aagatggttt aaataccccg
     4861 ttacttttga cttacactat acggtatcgc atcgtttaat attcgcaccg gccagatttt
     4921 tatttctatt agttgtcaca atactgaatg cgtacgacca cagtattctg gctcctgtgt
     4981 ggttatgctt taattctgcg ttccgggcag ataagcagtt gcttgcagga atccttcttg
     5041 tgttaatgtc agttcccctt ttaccagtgc tgatttccac attccgtcca acagagctta
     5101 tagcctttcc ctggattata gcattgtccg gctgaagttc tttttgaata ataatagaag
     5161 cactgctggc agatccagtc cgtttttcat aacccactgt actgataacc ataatctaat
     5221 cagtagaaat tgagtcgaaa ataagcacta ctccatacag gataattaga ggtcagtttg
     5281 attattcaca attc
//
LOCUS       DAZGTR010000219         5109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGTR010000219 DAZGTR010000000
VERSION     DAZGTR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.221"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..189
                     /locus_tag="ACHLCY_004782"
     CDS             <1..189
                     /locus_tag="ACHLCY_004782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371863.1"
                     /translation="YLMFVGEHSPNQDKSAGNGFERCEATARRVAGRTPAINCQTSNQ
                     AKRPSERMAFLLFELTFN"
     gene            complement(186..944)
                     /gene="yhdZ"
                     /locus_tag="ACHLCY_004783"
     CDS             complement(186..944)
                     /gene="yhdZ"
                     /locus_tag="ACHLCY_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP0371864.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(952..2055)
                     /gene="yhdY"
                     /locus_tag="ACHLCY_004784"
     CDS             complement(952..2055)
                     /gene="yhdY"
                     /locus_tag="ACHLCY_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0371865.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRRRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2065..3246)
                     /gene="yhdX"
                     /locus_tag="ACHLCY_004785"
     CDS             complement(2065..3246)
                     /gene="yhdX"
                     /locus_tag="ACHLCY_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0371866.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3314..4339)
                     /locus_tag="ACHLCY_004786"
     CDS             complement(3314..4339)
                     /locus_tag="ACHLCY_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0371867.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4770..4991)
                     /locus_tag="ACHLCY_004787"
     CDS             complement(4770..4991)
                     /locus_tag="ACHLCY_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371868.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1417 a         1325 c         1201 g         1166 t
ORIGIN      
        1 tatttgatgt ttgtcggtga acactctccc aaccaggaca aatccgccgg gaacggattt
       61 gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
      121 acatcaaatc aagcaaaaag gccatccgaa aggatggcct ttttgctttt cgaactaaca
      181 ttcaattaat ggattacctg cgataaaaat gccctcgtac gctctgattt aggatgcgta
      241 aaaaattcat caggtgcagc ttgctccact atttccccac gatccataaa aatcacccgg
      301 tcagcgacgg ttcgcgcaaa ccccatctca tgcgttacac acaacattgt catacccgat
      361 tgcgccagcc caatcatcgt atccagcacc tctttcacca tctccggatc gagcgccgac
      421 gttggctcat caaacaacat aattttcggc ttcatacaca gcgaacgcgc aatggcaacg
      481 cgttgctgct gaccaccaga aatttgtccg ggaaacttat gcgcatgttc ggcaattctc
      541 acccgctcaa ggtaatgcat cgccagagct tcagcctctt tctttggcat cttgcgtacc
      601 caaatcggtg ccagggtaca gttctgtaga acggtcagat gagggaagag attgaaatgc
      661 tgaaagacca ttcccacttc ctgccggacg cgctcaatat tgcggatatc ttcattaagt
      721 tcgatgccat ctaccacgat ccgtccctgt tgatgttctt ccagatgatt aatacaacga
      781 atggttgtcg atttaccgga acctgaaggg ccacacagaa cgatccgttc tcccggttgc
      841 acggttaaat ttatattttt caaaacatgg aattgtccat accatttatt gacgttttcc
      901 agcgtaatca tcgcgttagc aggttgcagt aaaatttggc tcatagtgtc ctcaatgcgg
      961 tgtacgcccg gtgttaaaac gtttttccag atgctggcta tagcgcgaca tgctgaaaca
     1021 gaagatccag tagatcagcg cggcgaaaac atacccttcc gtcgacatac ccaaccaggt
     1081 gggatcaacg gttgcctgct gaacgctact gaaaagatcg aacaacccga taatgatcac
     1141 caggctggta tctttgaaga gtgcgatgat ggtatttacc agcccaggaa ttaccagctt
     1201 caacgcctgt ggcagaataa ccagcccctg agttttccag taacccaacg ccagcgactc
     1261 tgccgcttca tactgccctt taggcagtgc ctgtaatccg cctcgcacga cttccgcaac
     1321 atatgctgac tgaaacagga tcacgccaac cagcgcgcgg atcagtttgt cgatactggt
     1381 gccttctgcc ataaacaacg gcagcatgac cgaagacata aacaatacgg taatcagcgg
     1441 tacgccgcgc cagaactcga taaaaatgac cgataagata cgcacaatcg gcatatggga
     1501 gcggcgacct aacgccagta agatccccca cggtagcgcc cccgcaatcc caacggatgc
     1561 aataattaaa gttagcgtca gcccgcccca ttgccgggtt tcaacccgct ccagaccaaa
     1621 aaaaccgcca tacatcagcc accagacaat cagtgggtaa atcaccgccc agaccgcaat
     1681 atagcgaccg cgatgcggga gtattttcca gaacattggt gcgattgaca caaggccaat
     1741 cagtaatgcc aggttaattc gccggcgttg gtcgtgtggg taaagtccat acataaactg
     1801 accaaatcgt tcgtggatga agacccaaca ggcaccggct tttgtgcagt cggcacgcgt
     1861 tgagccaacc caattagcct gcaaaaatgc ccagttcagc aacggtggga tcagttccca
     1921 catcaaccat atgcagccaa tagtcagcag gctattgctc cagctggaga acagattttt
     1981 tcgcacccac accatcgcgc ggcttgagtt atggctcgct gggcgcgagg gctgagacag
     2041 caatactttt gtcatcatga ctccttagcg ttcaacgatc gcgatgcggc ggttatagat
     2101 attcatcagc agcgagatag tcaggctgat aatcagatag accgacatgg tcatggctat
     2161 cgtctcgatg gcttgccccg tctggttcag cacggtgcca gcaaacagcg aaaccatatc
     2221 gggatagcca atagcggcgg caagagagga gtttttgacg atgttgagat actggctggt
     2281 taacggcgga ataatcactc gcagtgcctg gggaataatg acctggcgta gcgtaaccgg
     2341 gttgggtaat cccagtgacc gagccgcttc atgttgacca taaggcactg cctggatccc
     2401 agcgcggata atctcggcga taaatgcaga tgtataaacc gaaagtgcca gcgttaaggc
     2461 tgccagttca ggaattaaaa ccatcccgcc gcggaagtta aagcctcgta gggctgggac
     2521 atcccagtgt agtgctgcgc caaaaagcca ttgcgccagt aaaggcaaac caatgatcaa
     2581 cactgcggcg atcggccagg tgcggcgcag ttgtccggtc tttatctggt atgttttatt
     2641 aaagcggaat agcccaacag aaaggactat agccataaca acagccagga taaacgcaat
     2701 aaatccgtct cccagctgcg gtgacggaat ataaagccca cgattgctca aaaaggccag
     2761 atcaaacgcg ctcacagcct ggcgtggtcc gggcaaattg cgcaacacgg caaagtacca
     2821 gaagaagatt tgcagcagcg ggggaatatt acggaagatc tcaatataaa ttgtggaaag
     2881 ctttcgtagc agccagttat ccgaaagtct cgccagaccg ataaagaagc ccaaaacaga
     2941 agcgaacaca atacacaatg cagaaaccag tagcgtattg agtaagccga caataaaaac
     3001 gcgtccgtag gtgtcgccct gctggtaatc gatcaaatgc tggacaatac cgaagccagc
     3061 gccgcgatcc agaaaggcaa aacctgaagt aatgccacga ttactgagat tcgttacagt
     3121 gttgtgaaat aaccaaccaa caatgccgac aacagcaaca acggcaagga tctggaataa
     3181 ccaggcacga accgtagggt tggcaaagga gagtgagcct ttaacggttg agcggcgatg
     3241 agacatagaa acctcggaaa gcataacgct gaagagagcg ccgccgtagc gacgctcgtt
     3301 acctcacacc gacttaacgc acgggcggcg cgtactgaat accgccgtta ttccagagat
     3361 tattttgccc acgtttaatt ttcagcgggc tttctgaacc tacattacgc tcgaaaattt
     3421 ccgagtagtt acccacctgt ttgatgatgt tataggccca tttattatcc aacttcagat
     3481 ccttgccgta atcgccttct ttacccagca gatgtgccat atcaggcgtt gctggattag
     3541 ccgctttttc atcgacgttc tgggaattga tgcccatctc ttcagcattc agcatggcga
     3601 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3661 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3721 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3781 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3841 ccttgaaata gtcggcaacg ttgagttcgg tatcagtacc cgcctgaata cagacggtag
     3901 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     3961 cgtcgtaata agtgacgcca gtaaatgcca ttcccatccc ggcgtcgcga gatgaagtcc
     4021 aggtcgtatt acgggagagc aaatccactt cccctgactg taaagccgtg aagcgttctt
     4081 ttgctgtgag tggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4141 cacgacaaac atcaacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4201 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgaacg gcatcaagcg
     4261 tcgctccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4321 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4381 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccagcttt
     4441 tgcgcccgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4501 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4561 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggaag tgtgaaagtt
     4621 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaatcagcag
     4681 agaattagcc agcagaatat agcaatattt tatcttatgt aaagatactg tatcgttaaa
     4741 cagagcgcga aactccgcgc cctgttcagt tatttcgaca gagcgatgat ccccatcgtc
     4801 aggccagcaa cagccgctgc cccgccggca atagccccag ccgtttccca gttatcctga
     4861 gtcgtgcctt tcatacaggt attggtgtga accagacgac cctgatcgtc ataaaccgga
     4921 acgcagggcg aatcgtgagc acagcccgcc agcaatgcgg tgagcaatgc cacaggaatt
     4981 aatcttttca tggtgttacc tcagtaataa acgctaacgc ttaataagtt aactctctta
     5041 ttaacgagct gaatgccatt ttatcaccgg aggttataaa tattgcggaa tgaataatga
     5101 caaattatg
//
LOCUS       DAZGTR010000220         5056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGTR010000220 DAZGTR010000000
VERSION     DAZGTR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.222"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            308..835
                     /locus_tag="ACHLCY_004788"
     CDS             308..835
                     /locus_tag="ACHLCY_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0371869.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEIREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGTQTQPEEAQQFSGQPQPESQPEPKKGGAKTKGRGRKAAQPEPL
                     QPSEPAYDFDDDIPF"
     gene            891..1124
                     /locus_tag="ACHLCY_004789"
     CDS             891..1124
                     /locus_tag="ACHLCY_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFP0371870.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
     gene            1183..3141
                     /locus_tag="ACHLCY_004790"
     CDS             1183..3141
                     /locus_tag="ACHLCY_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0371871.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERDIIQADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEVACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEFLREAEGWGWCAGRMEPVGECREDARAYRNLPEPEAVLTEAEE
                     EHLNELMARYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CIQRGVQLRSEDDVADDADRTEQGLESASVEEISLPLLTKLTSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSKAHSSPARIRLACEHYALTSDAPSGKEGAAFMALMAEHSRF
                     AALLPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECCHTSRSPLDTLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDTTERTDNLADAA"
     gene            3196..3630
                     /gene="psiB"
                     /locus_tag="ACHLCY_004791"
     CDS             3196..3630
                     /gene="psiB"
                     /locus_tag="ACHLCY_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0371872.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQA"
     gene            3627..4389
                     /locus_tag="ACHLCY_004792"
                     /pseudo
     CDS             3627..4389
                     /locus_tag="ACHLCY_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(4358..4546)
                     /locus_tag="ACHLCY_004793"
     CDS             complement(4358..4546)
                     /locus_tag="ACHLCY_004793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371873.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            4568..4717
                     /gene="mok"
                     /locus_tag="ACHLCY_004794"
                     /pseudo
     CDS             4568..4717
                     /gene="mok"
                     /locus_tag="ACHLCY_004794"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            4659..4784
                     /locus_tag="ACHLCY_004795"
     CDS             4659..4784
                     /locus_tag="ACHLCY_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP0371874.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
BASE COUNT         1140 a         1322 c         1576 g         1018 t
ORIGIN      
        1 tgccattcac ctgtttaagc ccctgtcgcc gggggctgat acagccacaa cctgccgcac
       61 tgactgtcca gggtaaatga acagcccgca aaatttttta cccaaaaccc ggtaaaaaat
      121 tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag tgatgcccca tgcggcgaag
      181 gttctcaaac aggtgatggt gaataccgga gggaacaaag agcaggtccc cgcaaaacgt
      241 caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaacatca acagaaggag
      301 acacatcatg gcagttcgtg gcattaacaa ggtcattctt gtcggtcgtc tgggaaaaga
      361 tccggaagtc cgttacatcc caaacggggg cgcagtggca aacctgcagg tggccacgtc
      421 agaaagctgg cgcgacaaac agacggggga gatacgggag cagacagaat ggcatcgcgt
      481 ggtgctgttc ggcaagctcg cggaagtggc tggtgagtat ctgcgcaagg gcgcgcaggt
      541 ctacatcgaa ggtcagcttc gcacccgtag ctgggaggat aacggtatca cccgttacgt
      601 caccgaaatt cttgttaaga ccacgggcac catgcagatg ctgggacgtg ccgcaggtac
      661 tcagactcag ccggaagagg cgcaacagtt cagcggtcag cctcagccgg aatcacagcc
      721 ggaaccgaaa aaaggaggcg cgaaaacgaa aggccgtgga cgtaaggccg cgcagccgga
      781 gcctctgcag ccgtctgaac ccgcgtatga cttcgatgac gacattccgt tctaacgaaa
      841 gacagtgaca accgccccgt ccagtgcggg gcatcaccgg agagatgagg atgagcgaat
      901 atttcagaat acttcaggga ctgccggacg gtccttttac ccgcaaacag gccgaagccg
      961 ttgccgcaca gtaccggaat gtctttatcg aggatgatca gggaacgcat tttcgcctgg
     1021 ttgtccgtca ggatggcacg ttgatctggc gctcctggaa ttttgaggac tgtgccgggt
     1081 actggatgaa ccggtacatc agggatttcg ggattcttaa gtaagagagg tgccggacgc
     1141 gcgaacgtcc ggcaggacat aagcaattat aaggggatga ttatgcctgt aacgaagtgt
     1201 gaaccagaaa ctacccgcaa agcaagccgt aaatctgtaa aaacgcagga aactgcactg
     1261 tctgccctgc tggcgcagac ggaggaagtg agcgtgccgc tggattcact gattaaatca
     1321 ccgctgaatg tgcgcacggt gccgtattct gcggagtccg tcagtgaact ggctgattcc
     1381 attaagggag tcggcctgct gcagaatctg gttgttcatg ccctgccagg tgaccgttac
     1441 ggtgtcgccg caggtggtcg ccgactggca gcactcaaca tgctggcaga acgtgacatc
     1501 attcaggctg actggcctgt ccgtgtgaag gtcattccgc aggagctggc gactgccgca
     1561 tcgatgaccg agaacggtca tcgtcgggat atgcaccctg ccgaacagat tgccggattc
     1621 cgcgcaatgg cgcaggaagg caaaacacct gcacaaatcg gtgatttgct gggctattca
     1681 ccccgccacg ttcagcgaat gctgaaactg gctgaccttg cgcctgtcat cctcgatgcg
     1741 ctggcagaag accgcatcac caccgaacac tgtcaggcgc tggcgctgga gaacgacacc
     1801 gcgcgtcagg tgcaggtgtt tgaagtcgcc tgtcagtcgg gatggggcgg taaaccggaa
     1861 gtacagacca ttcgtcgtct ggtgaccgaa agtgaagtgg cggtggcggg gaacagtaaa
     1921 ttccgcttcg tgggggctga tgccttctcg ccagacgaac tgcgcaccga tttgttcagc
     1981 gatgacgggg acggttatgt cgaccgcgtg gcgctcgatg ctgccctgct ggaaaaactc
     2041 caggctgtcg cagagttcct tcgggaagcc gagggctggg gatggtgcgc cggacgcatg
     2101 gagcctgtcg gtgaatgccg tgaagatgcc cgggcatacc gcaacctgcc ggagccggaa
     2161 gcggtgctga cggaggcgga agaagaacac ctgaacgaac tgatggcacg ttacgacgcg
     2221 ctggaaaacc agtgtgagga atctgacctg ctggcagcag aaatgaagct gattgactgc
     2281 atggcgaagg tcagggcgtg gacgccggag atgcgtgccg gaagtggcgt ggtggtgtcc
     2341 tggcgttacg gtaatgtgtg tatccagcgt ggtgtgcagt tgcgcagtga ggatgatgtg
     2401 gctgacgacg ctgaccgcac ggaacagggg ctggagagcg cgtcagtgga ggaaatcagt
     2461 ctgccgttgc tgactaaact gacctcagag cgcacgctgg cagtccaggc agcactgatg
     2521 cagcaaccgg acaaatccct ggcactgctg gcatggacgc tctgcctgaa tgtgtttgac
     2581 agcaaagcgc acagtagtcc cgccagaatt cgcctggcat gtgagcatta tgcgctgacc
     2641 agcgatgcac cgtcggggaa ggaaggtgcc gcattcatgg cgctgatggc agaacactct
     2701 cgttttgctg ccctgctgcc ggaaggatgg gaacgggata tgacgacgtt cctgtcactc
     2761 agccaggagg tgctgttatc cctgctcagc ttctgtaccg cgtgcagtct taacggtgtc
     2821 cagacccgag agtgttgtca cacgtcacgc agtccgcttg acacgctgga aaccgccatc
     2881 ggctttcaca tgcgcgactg gtggcagccg acaaaagcaa acttcttcgg acacctgaaa
     2941 aagccgcaga ttatcgcagc cctgaatgag gcaggactgt ccggtgccgc acgggacgcg
     3001 gagaagatga agaaaggcga tgcggctgaa catgcagagt tccatatgaa agacaaccgc
     3061 tgggtgcctg gctggatgtg tgcaccacgt ccacagacgg ataccactga acgcaccgat
     3121 aacctggctg atgccgcctg atgaacaacc acaccgcccc gccggagacg gggcggcagc
     3181 aagggagata ccgtgatgaa aactgaactg accctgaatg tattacagac catgagtgca
     3241 caggaatatg aagatatccg ggctgccgga agtgatgaac gccgtgagct gacacacgcc
     3301 gtgatgcggg aactggatgc gccggacaac tggacgatga atggcgagta cggcagcgag
     3361 ttcggcggct ttttccccgt ccaggtccgt ttcacgcctg cccacgaacg ttttcacctg
     3421 gcgttatgtt caccgggcga tgtctctcag gtctgggtgc tggttctggt gaatgccggt
     3481 ggtgagccgt ttgcggtggt tcaggtgcag cggcggtttg caccggaagc tgtcagccat
     3541 tcactggcac tggcggcgtc actggatgcg caggggtaca gcgttaacga catcattcat
     3601 atcctgatgg cggaaggagg tcaggcatga gcgcacgttc acaggcactg gttcctctca
     3661 gcacagagca acaggccgca tggcgggcgg tggcggagac cgaaaaacgc cgccatcagg
     3721 gtaacacgct ggctgaatat ccgtatgcgg gtgcgttttt ccgctgtctg aacggaagcc
     3781 gcaggatatc gctgtctgac ctgcgatttt tcatgccgtc cctgaccgca gaagaactgc
     3841 acggcaaccg tcttcagtgg ctgtatgcca tcgatgtgct gattgaaaca cagggcgagg
     3901 tctgcctcct tccccttccc ggtgatgcag cagagcgctt gttcccgtcg gtgcggtttc
     3961 gtgtcaggga acgcagtcgc cataaatccg cgctggtcat gcagaagtac agccgccagc
     4021 aggcgaggga ggcagagcag aaagcccgtg cgtaccaggc gctggtcgct caggctgaaa
     4081 tcgaactggc attccattcg ccggaaacag tcggaagctg gcatgcacgc tggagtgacc
     4141 gggtggcaga gcatgacctg gaaaccctgt tctggcagtg gggcgagcgt tttccgtcac
     4201 tggccggaat ggaacgctgg cagtggcagg atatgccgtt ctggcaggtt attgcggaag
     4261 ccagtcttgc agcaagagag gccggtcacg ctgtccgtga gatggagcgg tggatggtgc
     4321 cgaacaaact ccgggaggca gcgtgatgcg gcaacaatca cacggatttc ccgtgaacgg
     4381 tctgaatgag cggattattt tcagggaaag tgagtgtggt cagcgtgcag gtatatgggc
     4441 tatgatgtgc ccggcgcttg aggctttctg cctcatgacg tgaaggtggt ttgttgccgt
     4501 gttgtgtggc agaaagaaga tagccccgta gtaagttaat tttcattaac caccacgagg
     4561 catccctatg tctagtccac atcaggatag cctcttaccg cgctttgcgc aaggagaaga
     4621 aggccatgaa actaccacga agttcccttg tctggtgtgt gttgatcgtg tgtctcacac
     4681 tgttgatatt cacttatctg acacgaaaat cgctgtgcga gattcgttac agagacggac
     4741 acagggaggt ggcggctttc atggcttacg aatccggtaa gtagcaacct ggaggcgggc
     4801 gcaggcccgc cttttcagga ctgatgctgg tctgactact gaagcgcctt tataaagggg
     4861 ctgctggttc gccggtagcc cctttctcct tgctgatgtt gtacgggcat gaacaacctg
     4921 acttcaggaa ggtcgtttcc cttcaggaac ggggatgaac gcgcgcctgc ggcgcccgtt
     4981 cttttccccc gccttctctg gttatggcct gtcagaaatc acatcacctt ccctgtgatt
     5041 attctctttt tcttcc
//
LOCUS       DAZGTR010000221         5050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGTR010000221 DAZGTR010000000
VERSION     DAZGTR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.223"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..745)
                     /gene="phsC"
                     /locus_tag="ACHLCY_004796"
                     /pseudo
     CDS             complement(1..745)
                     /gene="phsC"
                     /locus_tag="ACHLCY_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(742..1410)
                     /locus_tag="ACHLCY_004797"
     CDS             complement(742..1410)
                     /locus_tag="ACHLCY_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0371875.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACSKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKLHLYRRFGQHLIK
                     KENV"
     gene            complement(1474..2112)
                     /locus_tag="ACHLCY_004798"
     CDS             complement(1474..2112)
                     /locus_tag="ACHLCY_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP0371876.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(2125..4227)
                     /locus_tag="ACHLCY_004799"
     CDS             complement(2125..4227)
                     /locus_tag="ACHLCY_004799"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP0371877.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(4248..4874)
                     /locus_tag="ACHLCY_004800"
     CDS             complement(4248..4874)
                     /locus_tag="ACHLCY_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP0371878.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         1306 a         1359 c         1113 g         1272 t
ORIGIN      
        1 gtgtggcgta cgccccgtgg tgcaaagata aagatgaccg aagataaaaa aagaggctta
       61 taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc
      121 ccacggcttg cggatagaga cacagcagcc ccgtcaatag taacaacggc agcaatccat
      181 acataacacc aacataggcg acctgctgta agggattaaa tttagactgg gttgttgccg
      241 ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg
      301 ctcgctccag ccacccctga cgacgaatgc gatagtggtg accattaccc ccaacggcat
      361 tgatcagcac aaagccgagc cagcatgcga gtaacaaaaa tccgcaaact tcatgcaccg
      421 caaccagact tttaaccgca gttgcgccca ccagcgcaaa gtgatttatc agcccacttg
      481 ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgcagt
      541 aaagataaat cttctcacca taatcggtga ctgatttctt cacgccacga gcacgaagca
      601 aggcatgcag ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc
      661 acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg ctctgaaact
      721 gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg
      781 gcgatacaga tgcagtttcc cggcaccagg tagctgatat tgatagtatt tattttgctg
      841 caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccgggca
      901 ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca
      961 tttatctgcc actttagtga cagggttaag gtagcgaacc tggtacggac aagcgccaat
     1021 gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg
     1081 ccacgacgcc cctgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga
     1141 ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat
     1201 cgataaacgg cttccctgag cggggacgtg gttagtttta ctgcatgcgc gggcgcaaat
     1261 attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg
     1321 cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc
     1381 cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag tggctgcaac
     1441 tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc
     1501 gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta
     1561 caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat
     1621 cgcggcgatc gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat
     1681 ggcgactttg taaggacgag gatatgtctg gcactcaaaa cgaacggcgt gtgcaatggc
     1741 gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc
     1801 ctgcgttgag taatagtaat catcctggct tcccgtcata caagcgatat ccgcgtagtc
     1861 tccgttctgc ttaatgcttt ccagcaaaac tgacagctcc tcttctgcaa ccgaataggg
     1921 tggcgagaga aacacggcgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa
     1981 ctctgccagg atttcagccg gttctggttc ggttaccgta tcaacggggt gacgcatccc
     2041 ctgtaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc
     2101 ccgatggttc atctcaccca ttccttacac aggcagtaga ttgtgtgctg ccagatcggc
     2161 ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gtcggacaac caagctgtgg
     2221 gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat
     2281 tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaata cccaggaaca
     2341 gataagatcg tgttcattac gcatatcttt ggtttgcatc agttttaccg tgtaggcacg
     2401 atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc
     2461 ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt
     2521 cagcggcgaa acggtcattg gccagaccca gttgcacagg gtgacggcgt tatgcaaaca
     2581 gacccgcaac agcgaccact ttgcatattt aatttttgcg tcattgattg gcgtgtagtt
     2641 tttggtttca tcgtaggcat cttcagaacc aaaaagctct ttcgccactt cacgctgcag
     2701 tttcaatggc aaacccgaac caataaaatt gatatgggta tgcgtcatgc agtcacggtt
     2761 gaacatacag ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg
     2821 atagccaaac ggcgaccaga gtttattttt tgcgtagccc cagtactctt cacccaaatt
     2881 ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac ccacacgatg
     2941 cgccagacgg tagtaaaaat ctttaatgaa attaacgtca cccgcttcca gttgatccca
     3001 gtgaatttcc gcatactctt cagctggcag aacacgcttg aacacccctt tgctgtaaca
     3061 ataagtaaag tcgcgatgca gctgcccgta gttacaccat aggccgtagt catcgaacaa
     3121 attcagacca accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg
     3181 cccgttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac cacctgtgct
     3241 gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca
     3301 agagtggcag ccactcattt ttactgtgta tttctctgcc gccggaccta agtcaaaaac
     3361 ggatttatag gtacgaaagc cgactgtgtt ctgattgcct ggcggaattt cccccgtttc
     3421 aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc aacgtgactt
     3481 gggatctgaa tactctgccc acgattgcgg cgtacttggc acgacatggt tgttattcgc
     3541 accaataagt tcagtcatca tgtaatcgtt gagacgcttc atctcctgac gatcggcaat
     3601 gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccataattgc
     3661 gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaacaag
     3721 gttttcccca gcctgaccaa tagccgccac acaggtttcc ggactggtca atcgacaaat
     3781 ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt ccaggctaac
     3841 tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac
     3901 gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg catcgacaac
     3961 taaatttcct ttggtaaaag tagaaagtga ggtgatattt acgcgagaac tacagggggc
     4021 accagatcca gttaatgggc cggtagcaaa gactaattta ttcgcttcat cgaaaggttt
     4081 cgtgcctggc ggtacttcgt catacataat tttgtagccg aagcccatgc caccgacaaa
     4141 acttttaaac ttactggaat cttcgagggt aatatttcct gtcgtgagat tgactcttaa
     4201 tatattacct gtccaaccgt tagccatgat tttttccttt gcaagattca cacagtaata
     4261 tctttccact cgataatttt taacgcccct gttgggcagg cgtttgcgca ttcaccgcat
     4321 aacacacatt tcgaggattt tttactttcg gtatttacgg tggccatcat ccacgggcat
     4381 gccgtagtac aggcgctaca gccaatacag cgtttatgat cgacggtaat acagccttct
     4441 ttctgctgcc aggtaatcgc accaatcggg cagacgttca tgcattgcgg ctctttgcat
     4501 tgacggcagg tgtccgcggt atagttgaga tcgccataca ggccgccgcc agagccaacc
     4561 ccgttgtcgc caaagaaata attgcggtgg attttgatac gggagaagaa tgtccctact
     4621 gagccatcgt tgaagttggt acaagagatt tcacagcggt ggcaacccgt gcatcgcgct
     4681 cgctgggtca ccagcacccc tttgggtgta tttatcaacc ctactgtgcc gctatcaata
     4741 tcttcctgtt tgcaacccaa aagtgacagt aacgcagggg caattgttag accagcaagg
     4801 ccttttcctg atatacgcaa gaattctaat cgcgttaagc caatatctaa tagtggacga
     4861 tcaaccgggt tcatttattt cgcatcctct tcgcaaacac agtgctgaca attcagcagc
     4921 ctgatattaa gcattcaact aatacgctgc tgactctgtc cccaacacca gatattttcc
     4981 ctaacccgat agtgtatgag aaaagattta acgccgataa actaatcctt tatggttagg
     5041 agaaaaaata
//
LOCUS       DAZGTR010000222         5049 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGTR010000222 DAZGTR010000000
VERSION     DAZGTR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.224"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            276..4241
                     /locus_tag="ACHLCY_004801"
     CDS             276..4241
                     /locus_tag="ACHLCY_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0371879.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSFRTSAKKKKNKLMLILC
                     CLGILSPTYTFASRMDASNFYIRDYLDFAQNKGIFQAGATNIEIIKKDGSTLKLPEVP
                     FPDFSPVANKGSTTSIGGAYSITATHNTKTHHSVAAQNWGNSTYKQVDWYTSQPDFAV
                     SRLDKYVVETRGATEGADTSLSTQQALERYGVNYKGEKKLIAFRAGSGKIGIRKDGNL
                     TPLDDISYKPEMLNGSFVHIDNWSGWLVLTNNQFDEFNNLATQGDSGSALFVYDNQKK
                     KWVVVGTAWGVYNYSNGKNHTAYSRWDQKAIDNLKKNFSYNVDMSGVQQVTIENGKLT
                     GTGSDTTDIKNKDLIFTGGGNILLKSSFDNGAGGLVFNDKKTYQVNGEDFTFKGAGVD
                     TRNGSTVEWNIRYDNKDNLHKIGNGTLDVRKSQNTNLKIGEGLVILGAEKTFNNIYMA
                     SGDGTVKLNANNALNGDDYAGVFFTENGGTLDLNGYDQVFKYIAATDSGTTITNTNKD
                     KPATLSINNTDSYIYHGNITGNTKITHSFDQKQQNDRLILDGNINTTNDISIKNAHLV
                     MQGHATNHAVFRDGGFSCSFPAQLKWLCGTDYVAVIQNQEKSVNQKQNTDYKTNNLVS
                     DLSQPDWERRTFNFGTLHLENADFAVARNADVKGNIYAKNSSVMLGSDIAYIDLHSGK
                     NIINDGFSFRKDIRSGISESTAGDLSSFTGRVVADNSTLAINNKFLGEFTAENKSKVS
                     VKSRDVVLNTGATISNDSTLTLEKDSRLTANMWLMNSGTINVGENAELNIQGYPIADK
                     FIPSIHDLGNVKMTASNATLTAGNYAMFSGELTADNATAVRVNLGSETSTLSEFNPNP
                     ELTDLMFDKYNTSWTGKISALKGDASMVNTVWRMTGDSGLNTLKTSKSLTVFSSDNKF
                     STLTVNDLTTSDSTFVLRSDSTGSDKVVVKNKLEGKNNNLLVDYVANDGKHNSLNFEL
                     VSAPKGTAADVFNSQTQNVGFSDVTPVIEQKDSGEKTTWTLKGFNAVANQQSAEKAEN
                     FMSAGYKNFLAEVNNLNKRMGDLRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGV
                     DKKHELDGLDLFTGFTVTHTDSSASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKY
                     VHHDNEYTATFAGLGTRDYSTHSWYAGAEAGYRYHVTEDAWIEPQAELVYGSVSGKQF
                     AWKDQGMHLSMKDKDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVL
                     RDASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYS
                     F"
     gene            4748..5002
                     /locus_tag="ACHLCY_004802"
     CDS             4748..5002
                     /locus_tag="ACHLCY_004802"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371880.1"
                     /translation="MNDGRQTPPPPEAWKIRFLANGTPGTWFYTDDAAVTKECLRTHC
                     DDVEVIPLCRMHDGPVFREHHINRVGSLAAAACHGLKLPL"
BASE COUNT         1557 a         1090 c         1127 g         1275 t
ORIGIN      
        1 aatataaaaa ttctctaata aacaagcgtg atgcacactc ttcatctttc atagcatctc
       61 acctctttaa taagaatcat tattatttgc acatcttcct ttcatgcatc attagtgaac
      121 gactgagata aacataccgt atccgttatc catatgataa caaatacata aaagagtaaa
      181 catttatctg aacgatgatt acaatgttca caactgtcag agatgcacct ctgacaggtt
      241 aacaaaacta atgtttctct taaaacggag cttatatgaa taaaatatac tctcttaaat
      301 acagtcatat cactggaggg ttaattgctg tttctgaact atcaggcaga gtatcattca
      361 gaacatcagc caagaagaaa aagaataagc tcatgcttat attatgttgt ttaggaatat
      421 taagcccaac atacaccttt gcttctcgca tggatgcatc taatttttac atccgcgatt
      481 acctggattt tgcgcaaaac aaaggcatat ttcaggctgg cgcaacaaat attgaaataa
      541 taaaaaaaga tggaagtaca ctaaaacttc cggaagtacc gtttcctgac ttctctcctg
      601 tagcaaataa aggctcaacc acatctattg gtggagcata cagtatcact gctacacata
      661 atacaaaaac ccaccactcc gttgcagcac aaaactgggg aaactctaca tacaaacagg
      721 tagactggta cacttcacaa ccagattttg ctgtatcccg acttgataag tacgttgttg
      781 aaaccagagg ggctacagaa ggcgcagata cgtcgttatc aacacagcag gctctcgaac
      841 gttatggcgt taattataaa ggcgagaaga aacttattgc attcagagcc ggctcaggaa
      901 aaattgggat tagaaaagat ggaaatttaa cacctctgga tgatatctcg tataagcctg
      961 aaatgttaaa tggctctttc gttcatattg ataactggag tggatggtta gtgttaacca
     1021 acaaccagtt tgatgagttt aataaccttg caactcaggg tgacagtggt tcagcacttt
     1081 ttgtatatga caaccaaaag aaaaagtggg ttgtcgttgg aaccgcatgg ggcgtttata
     1141 actattcaaa tgggaaaaac catacagcat atagtagatg ggatcagaaa gccattgata
     1201 atctaaaaaa gaacttttct tacaatgtgg atatgtcagg agtacagcaa gttaccattg
     1261 aaaatggaaa actgacaggc actggctcag acaccaccga tataaaaaat aaggacttaa
     1321 tatttacagg cggtggaaac atcctcctga aatcctcttt tgataatggt gctggcggtc
     1381 ttgtctttaa tgataaaaag acctatcaag taaacgggga ggatttcacc tttaaaggtg
     1441 ccggtgttga cacaagaaac ggcagcaccg ttgagtggaa tatccggtat gataataaag
     1501 acaaccttca caagattggt aatggtacat tagatgtccg aaaatcccag aacaccaacc
     1561 tcaaaatagg tgaaggcctg gtcattcttg gtgctgaaaa aaccttcaac aatatctata
     1621 tggccagtgg cgatggtacc gttaagctga atgccaataa cgcactgaac ggtgatgatt
     1681 atgccggagt tttctttact gaaaatggag ggacccttga tctaaatggt tatgaccagg
     1741 tatttaagta tatagccgca acagattcag gaacaacgat tacaaacacg aataaagaca
     1801 agccagccac tttatctata aataatacag atagctatat ttaccatggc aatatcacgg
     1861 gcaataccaa aattacacat tcattcgacc agaaacaaca gaatgatcgt ctgattctgg
     1921 atggtaatat caatacgaca aatgatataa gcataaaaaa tgctcactta gtgatgcaag
     1981 ggcatgctac aaatcatgct gtattcaggg atggcggatt ttcctgttca tttcccgccc
     2041 agttaaaatg gttatgtggc acagattatg tcgcagtaat ccagaatcag gaaaaatcgg
     2101 ttaatcaaaa acaaaataca gactacaaaa ctaataacct ggtctctgat ttgtctcagc
     2161 ctgactggga aaggagaaca tttaacttcg gcacccttca tcttgaaaat gcggattttg
     2221 ctgttgcacg caatgctgac gtgaaaggta atatttatgc aaaaaactcc agcgtaatgc
     2281 ttggtagtga tatcgcttat attgaccttc attcaggaaa aaacatcatt aatgatggat
     2341 tttccttccg taaggatatt cgatccggaa tatcagaaag cacagcaggg gatttaagtt
     2401 catttaccgg acgtgttgtt gctgataatt caacgctcgc tattaacaat aagttcctgg
     2461 gagagtttac ggcagagaat aagagtaagg tttcggttaa aagccgtgat gtggtcctta
     2521 atactggtgc cacaatttca aatgacagta ctctgactct ggaaaaagac tcacgactga
     2581 cggctaatat gtggcttatg aactcaggca caattaacgt tggtgaaaat gcggagttaa
     2641 atattcaggg atatccgatt gctgataaat tcattccgtc aatacatgac ctggggaatg
     2701 tgaaaatgac ggcgtcaaat gccaccctga cggcaggtaa ttatgcaatg ttcagcggtg
     2761 aactcactgc cgataacgcc actgctgtca gagttaacct gggctcagag acatcaacac
     2821 tctctgaatt taatcctaat ccggaactga ccgacctgat gtttgataaa tataatacat
     2881 catggaccgg caaaatctct gccctgaaag gtgatgcatc aatggttaat accgtatggc
     2941 gtatgacggg agattccggg cttaataccc tcaaaaccag caaatccctg acggtattca
     3001 gcagtgataa taaattctcc acgctgacag ttaatgacct gacaaccagt gacagcacct
     3061 ttgtgctgcg ttctgactct acaggctctg ataaggtggt agtaaaaaac aaacttgagg
     3121 gtaaaaacaa taatcttctt gttgattatg ttgcaaatga tggaaaacat aattcgctaa
     3181 attttgaact ggtcagtgca ccgaaaggaa ctgctgccga tgtctttaat tcacagacac
     3241 aaaatgtggg cttcagtgat gtaacaccgg tgattgaaca gaaagatagc ggagagaaaa
     3301 ccacatggac cctgaaggga ttcaatgctg tagccaatca gcagtcagct gaaaaagcgg
     3361 aaaatttcat gtcagcaggc tataaaaatt ttcttgctga agtcaacaac ctgaacaaac
     3421 gtatgggtga cctgcgtgac atcaacggcg aagccggtgc atgggcacgc atcatgagcg
     3481 gtaccggctc tgccagtggt ggtttcagtg acaactacac gcacgttcag gtcggggtcg
     3541 acaaaaaaca tgagctggac ggactggatt tgtttaccgg tttcactgtc acacacactg
     3601 acagcagtgc ctccgccgat gttttcagcg gtaaaacgaa gtctgtgggg gctggcctgt
     3661 atgcttccgc catgtttgat tccggtgcct atatcgacct gattggcaag tatgttcacc
     3721 atgataatga gtacacagca acctttgccg gactcggaac ccgtgattac agcacgcatt
     3781 catggtatgc cggtgcagaa gcgggctacc gctatcatgt cactgaggat gcctggattg
     3841 agccacaggc tgagctggtt tacggttctg tatccggtaa acagtttgca tggaaggacc
     3901 agggaatgca tctgtccatg aaggacaagg actacaatcc gctgattggc cgaaccggtg
     3961 tagatgtggg taaatccttc tctggtaagg actggaaagt gacagcccgg gccggcctgg
     4021 gctaccagtt cgacctgctg gctaacggcg aaaccgtatt gcgggatgca tctggtgaaa
     4081 aacgcatcaa aggtgaaaag gacagccgta tgctgatgtc cgttggcctg aatgcagaaa
     4141 tcagggacaa cgtccgcttt ggactggagt ttgagaaatc cgcctttggt aagtacaacg
     4201 ttgataatgc agtcaacgct aacttccgtt actcgttctg atactccgta ttcaaaaggg
     4261 cctgcgggcc ctttatgctc agtgcctgcc gccctgatat gtctgagaga gatttcaggg
     4321 atgtcagtcg actggtttac ttaactgcga tgactaaagc tggcagtaat gccggctttt
     4381 ttacggccgc acacgtcttc ctctcatccg gaactgtgat acaaatcctg catacaggat
     4441 tgaccgccgg ttcctgcaaa tggcacattt tcagtaagat agcgaaggtt acattccccc
     4501 gtcatgtgtg gcacaagctg tcaccgggaa agaaacggaa agcacatgaa aaacatcaaa
     4561 actcacaccg gactccttat ccacaaagaa caaacgcggc gcgttcgtct tcatgaaaca
     4621 cccactgcct ggtgccacac gcaccgggag tgctacagca aaacgaccgg tcgccgctgc
     4681 ggcagccctg acagtctcag caggctgatt ctgagcagca ttcagcccat agaatgagat
     4741 accccctatg aatgatggca gacagacgcc tccgcccccc gaggcatgga aaatccggtt
     4801 tctggctaac ggtacacccg gaacatggtt ttacaccgat gatgcagccg tcacaaaaga
     4861 atgtctgcgg acacattgtg acgatgtgga agtgatcccc ctgtgccgta tgcatgacgg
     4921 tcccgttttc cgggaacacc atattaatcg agtaggcagc ctggcggctg cggcttgtca
     4981 tggcctgaaa ttaccgttat aaaaacagac aatatgaacc gccccgggtt tcctggagag
     5041 tgttttatc
//
LOCUS       DAZGTR010000223         4870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGTR010000223 DAZGTR010000000
VERSION     DAZGTR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.225"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..3026
                     /gene="evgS"
                     /locus_tag="ACHLCY_004803"
     CDS             <1..3026
                     /gene="evgS"
                     /locus_tag="ACHLCY_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP0371881.1"
                     /translation="FTNLYQALASVSAGQNDYFIGSNIITSSMISRYFTHSLNVVKYY
                     NSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWLDTGNLAFLNKPLELTE
                     HEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFSPITVSH
                     NIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVFVMQKAPDSEQTLKKGM
                     KVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELDALVATQLNSRYMIDHY
                     YPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNV
                     TIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALS
                     DSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENSDSPFKDVFSNAHEVTA
                     ETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYICGWQDITETRDLINAL
                     EVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYA
                     TGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSS
                     TFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDS
                     GSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTF
                     TITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEA
                     TDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANERE
                     KGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQE
                     ILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITP
                     VSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(3082..4227)
                     /gene="yfdE"
                     /locus_tag="ACHLCY_004804"
     CDS             complement(3082..4227)
                     /gene="yfdE"
                     /locus_tag="ACHLCY_004804"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP0371882.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(4301..>4870)
                     /gene="yfdV"
                     /locus_tag="ACHLCY_004805"
     CDS             complement(4301..>4870)
                     /gene="yfdV"
                     /locus_tag="ACHLCY_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP0371883.1"
                     /translation="STGLVVAIISIIVNAITIPIGLYLLNPSSGADGKKNSNLSALIS
                     AAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLTLAAHKFEFS
                     AEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGIIIASRFNVYT
                     RTGTASLAVSVLGFVVTAPLWIYVSRLVS"
BASE COUNT         1478 a         1092 c          980 g         1320 t
ORIGIN      
        1 cttttacaaa tttatatcag gcattagcat ccgtatcagc cggacagaat gattacttta
       61 ttggtagtaa catcattacc agcagtatga tttcccgcta tttcactcac tccttaaatg
      121 tagtgaaata ttataactcg ccgcgtcaat ataatttttt cttgaccaga aaagaatccg
      181 tcattcttaa tgaagtactc aatagatttg ttgatgcttt aacaaatgaa gttcgctatg
      241 aagtttcaca aaattggctt gatacaggaa acctggcctt tctgaacaaa ccattagaac
      301 tcactgaaca tgaaaaacag tggattaagc aacatcccga tttaaaggtg ctggaaaatc
      361 cttactctcc tccctattct atgacggatg aaaatggctc ggttcggggc gttatggggg
      421 atattcttaa tattattacc ttgcaaacag gtttaaattt ttctccgatc accgtttcac
      481 acaatatcca tgccggaaca cagcttaacc ccggtggatg ggatataata cctggcgcta
      541 tttatagtga agatcgagaa aataatgttt catttgcaga agctttcata acaacgccat
      601 acgtttttgt catgcaaaaa gcacctgaca gtgaacaaac attaaaaaaa ggaatgaaag
      661 ttgccattcc atattattat gagctgcatt cgcaattaaa agagatgtat ccggaggttg
      721 aatggataca ggtcgataat gccagcgctg catttcacaa ggttaaggaa ggtgaacttg
      781 atgctctggt cgcgacacag ttaaattcgc gttacatgat cgatcattac tatcctaatg
      841 aactttatca ttttcttatt cctggcgttc cgaatgcatc gctttcgttc gcttttcctc
      901 gcggagaacc ggaacttaag gatattatta ataaagcact gaatgcaatt cccccaagcg
      961 aagttctgcg cctgacggaa aaatggatta aaatgcccaa tgtgaccatt gacacatggg
     1021 acctatatag cgagcaattt tatattgtta cgacattatc cgttttatta gttggcagta
     1081 gccttttatg gggattctac ctgttacgct cagttcgtcg tcgtaaagtc attcagggtg
     1141 atttagaaaa ccaaatatca ttccgaaaag cactctcgga ttccttaccg aatccaactt
     1201 atgttgtaaa ctggcaaggt aatgtcatta gtcataatag tgcttttgaa cattatttca
     1261 ctgcggatta ctacaaaaat gcaatgttac cattagaaaa cagtgactca ccctttaaag
     1321 atgttttttc taatgcgcat gaagtcacag cagaaacgaa agaaaatcga acaatataca
     1381 cacaggtatt tgaaattgat aatggcatcg agaaaagatg cattaatcac tggcatacat
     1441 tatgcaatct tcctgcaagt gacaatgcag tatatatttg tggttggcaa gatattactg
     1501 aaacgcgtga tctaattaat gcactcgagg tagaaaaaaa taaagcgata aaggctaccg
     1561 tagcaaaaag tcagtttctg gcaacgatga gtcacgaaat aagaacacca ataagctcta
     1621 ttatgggctt cctggaactt ctgtcgggtt ctggtcttag caaggagcaa cgggtggagg
     1681 cgatttcact tgcctacgcc accggacaat cactcctcgg cttaattggt gaaatccttg
     1741 atgtcgacaa aattgaatcg ggtaactatc aacttcaacc acaatgggtc gatatcccta
     1801 ctttagtcca gaacacttgt cactctttcg gtgcgattgc tgcaagcaaa tcgatcgcat
     1861 taagttgcag cagtacattt cctgatcatt acctggttaa gatcgaccct caggcgttta
     1921 agcaggtctt atcaaattta ctgagcaatg ctctcaaatt taccaccgag ggggcagtaa
     1981 aaattacgac ctccctggtt cacattgatg acaaccacgc tgttatcaaa atgacgatta
     2041 tggattctgg aagtggatta tcgcaggaag aacaacaaca actctttaaa cgctacagcc
     2101 aaacaagtgc aggtcgccag caaacaggat ctggcttagg cttaatgatc tgcaaagaat
     2161 taattaaaaa tatgcagggc gatttgtcat tagaaagtca tccaggcata ggaacaacat
     2221 ttacgatcac aatcccggta gaaattagcc agcaagtggc gactgtcgag gcaaaagcag
     2281 aacaccccat cacactacct gaaaagttga gcatattaat cgcggatgat catccgacca
     2341 acaggctatt actcaaacgc cagctaaatc tattaggata tgatgttgat gaagccactg
     2401 atggtgtgca agcgctacac aaagtcagta tgcaacatta tgatctgctt attactgacg
     2461 ttaatatgcc gaatatggat ggttttgagt tgactcgcaa actccgtgag caaaattctt
     2521 ccttacccat ctgggggctt acagccaacg cacaggctaa cgaacgtgaa aaagggttaa
     2581 gttgcggcat gaacttatgt ttgttcaaac cgttgaccct ggatgtactg aaaacacatt
     2641 taagtcagtt acaccaagtt gcgcatattg cacctcagta tcgccacctt gatatcgaag
     2701 ccctgaaaaa taatacggcg aacgatctac aactgatgca ggagattctc atgactttcc
     2761 agcatgaaac gcataaagat ctacccgctg cgtttcaagc actagaagct ggcgataaca
     2821 gaactttcca tcagtgtatt catcgcatcc acggtgcggc taacatcctg aatttgcaaa
     2881 agttgattaa tattagccat cagttagaaa taacacctgt ttcagatgac agtaagcctg
     2941 aaattcttca gttgctgaac tctgtaaaag aacacattgc agagctggac caggagattg
     3001 ctgttttctg tcagaaaaat gactaaatag cggctcccac aatgttcaaa tgtgggagct
     3061 atttaccagc acatcttaca tttatgatga gaactcctgg cgaatttgtt ccccatgctg
     3121 gtcgagcgtt gccgctcccg gcataacatg cgggtccgcg cagccgctga tttttatcgg
     3181 attacccggc atcattattc ccccggcttc aatcaacata tttctcgcct gagtttgtgg
     3241 caatttaatg gcctcagcca cacttaataa cggcgcgacg ggtacaccaa cttcatgtat
     3301 tctggctaac caaacttcag ctgcctgcgt ttttaacgtc cgctcaatat attgtttaag
     3361 aatagcctgg ttttgtacgc gtaaaatatt gctgctaaat cggggatcat taaccagttc
     3421 cgtaagctcc agtgcctggc ataacgcaga aaaaagcttg tcattaccac aacaaatcgt
     3481 aatcggctta tcctgagtat tgaaaacatc aaaaggtgcc atgtagggat ggcgatttcc
     3541 cagacgttgt ggtgacttcc ctgtcgcgat atatgccatc agaccatgct ccagaaaact
     3601 cagcgtggca tcaaacatcg ctatatcgac atgcgcccct ctctggctct tttcgcggcc
     3661 ataaagtgca ctcactattc cgctgaataa atagacaccg ccgcatagat ccgcaagaga
     3721 tgtaccaacg cgcactggcg gagcatcagg atatcctgtt tccatcatta tcccgctcat
     3781 tgcctgaatg atggtatcgt aggcaggagc atcttttagc ggaccggtat gtccgaaacc
     3841 tgacgatgaa gcatatatga ggcgcgggtt gatttcttga agcgtttccc atgaaaaccc
     3901 cagtttttcc attgtacctg ggcgaaaatt ctcagctaat acatcagctt gtttgagcat
     3961 atttataaat atacttttat cgtgatcatt ctttaaatca agaaccacac tctctttgcc
     4021 atgattaata aaactgtaat agagtgactg tccatccaca tagggaccaa atgtgcgggt
     4081 atcatcacca tgacccggcg gctcaacttt aattaccctt gcgcccatat tacaaagaag
     4141 ttgagttccg aaaggtccat taaggacatg tgtcatatcg ataactaata agccttcaaa
     4201 cggccctttg ctttcattat ttgtcatttg cttatgcctt gaaaaaagcc gaaggtgaaa
     4261 agacacttcg gcatttatgt aaaatcaata acatgattaa ttatgaaacc agtcgactga
     4321 cataaatcca caagggagcc gtgacgacaa agcccagtac gctcacagcc aatgacgctg
     4381 taccagtacg ggtgtagaca ttaaaccggc tggcaataat gattccggag aatgccggcg
     4441 gtaatgcgcc tgccagtacc atcatctgca gatgttcgct gttcaaatga catgccatac
     4501 caacgagaag cagtgccagt ggcatcagaa tcagcttcag gaaggtgtta taagcaattt
     4561 cggcactgaa ctcgaattta tgtgcagcca gagtcaaccc cgcagcgaat accgctaccc
     4621 ctgagttagc cttcgcaatc agattaaagg ttgggtccca tgctgccgga atttttaccc
     4681 caaccaacac caggatcgtt gccagaacag gtgcccatac tactggctcc tttgccgcag
     4741 aaattaatgc gctcagatta ctattcttct taccatccgc tcctgaggaa ggattcagca
     4801 aatacagacc aataggaata gtaattgcgt taacaataat agaaataatt gctaccacta
     4861 aacctgttga
//
LOCUS       DAZGTR010000224         4866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGTR010000224 DAZGTR010000000
VERSION     DAZGTR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.226"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            78..1994
                     /gene="mngA"
                     /locus_tag="ACHLCY_004806"
     CDS             78..1994
                     /gene="mngA"
                     /locus_tag="ACHLCY_004806"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP0371884.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     FNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLGSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLMWRRH
                     AVKHGNYLTDGVMP"
     gene            2012..4645
                     /gene="mngB"
                     /locus_tag="ACHLCY_004807"
     CDS             2012..4645
                     /gene="mngB"
                     /locus_tag="ACHLCY_004807"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP0371885.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADEDGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLIRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YEMAVPRNLQERSARQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLCVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            4670..>4866
                     /locus_tag="ACHLCY_004808"
     CDS             4670..>4866
                     /locus_tag="ACHLCY_004808"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371886.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNK"
BASE COUNT         1186 a         1140 c         1346 g         1194 t
ORIGIN      
        1 gtgaaattga tcacataatg gtattgtttt atcgggcaca ctggcgcgac tataaaaacg
       61 atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg ttgtgtctga
      121 atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt cttgctgctc
      181 tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt gaaagccttg
      241 gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct gcggtaaaag
      301 aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc gttgatggcc
      361 cggaagcggt tgatttagtg gtgctgctgg cgattccccc caatgaagcg ggtactacgc
      421 atatgcaact gctgacagcg ctgaccacgc gcctggcgga tgatgagatt cgggcgcgta
      481 tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag ggaggcacgc
      541 aaccttctgc ctcttttttc aacgcgccaa ctatcgtctg cgtaacggcc tgtccggcgg
      601 gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc aaactcggcg
      661 taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta acggcggatc
      721 aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag gagagtgagc
      781 gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc catgcagaag
      841 cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc gtacagcaag
      901 atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg agcggaatct
      961 cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg gtattactgt
     1021 cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg tggatgtacc
     1081 gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg gcctataccg
     1141 cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga cttgccgcca
     1201 acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc ggttacttga
     1261 tgcgctgggt gaaaaatcac ttgcgtcttg gcagtaaatt caatggcttc ctgacttttt
     1321 atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt gtggtgggag
     1381 aacctgtcgc atggatcaat aactcgctta ccgcctggct gaacggtctg tcaggaagta
     1441 acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc ctttgacctt ggagggccag
     1501 tgaataaagc cgcttatgca ttctgcctgg gcgcaatggc gaacggcgtt tacggcccgt
     1561 atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct tccacgatgc
     1621 tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc tggctgttag
     1681 ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg ctgcgggtta
     1741 ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg atgaatatcg
     1801 gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat aatggcgcgg
     1861 gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct gcaatctcga
     1921 ctgcaattct cctgatgtgg cggcgtcacg cggttaagca tggcaactat ctgactgatg
     1981 gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg ttcacatcac
     2041 cccgcatatg cactgggatc gggagtggta tttcaccacc gaagagtcac gcattctgct
     2101 ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat acaaatatta
     2161 cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac cggaaaacaa
     2221 agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct ggtataccca
     2281 gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt acggaatgcg
     2341 tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccggatt cctttggcat
     2401 gtccgggcag cttccgcata tctacaatgg atttggcatt acccgcacca tgttctggcg
     2461 tggatgttct gagcgccacg gtactgataa aaccgagttt ttgtggcaaa gcagtgacgg
     2521 tagcgaagtg acggcgcaag tgttgccgct gggctacgcc atcggtaagt acttacctgc
     2581 cgacgaagac ggattacgca agcgcctcga cagttatttt gatgtgctgg aaaaagcgtc
     2641 agtaaccaaa gaaattttat tgccgaatgg tcatgaccag atgccattgc agcaaaatat
     2701 cttcgaagtg atggataagc tacgtgagat ctaccctcaa cgtaagtttg tgatgagccg
     2761 ctttgaagag gtatttgaga agatcgaagc gcagcgagat aatctggcaa ccctgaaagg
     2821 ggaatttatt gatggcaaat atatgcgcgt gcatcgcacc atcggttcta cgcgtatgga
     2881 tatcaaaatt gcccacgcgc gtattgaaaa taagattgtt aatctgctgg aaccgctggc
     2941 aacactggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga aaatgtggaa
     3001 agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg acaaagttca
     3061 tcgcgaaatc gtcgcccgct tcgaactggc tgaagacatg gcggataatc tgattcgttt
     3121 ctacatgcgc aaaattgccg acaacatgcc gcagagcgac gccgacaaac tcgtcctgtt
     3181 taacctgatg ccctggccgc gtgaagaagt gatcaacacc actgtgcgcc tgcgcgccag
     3241 ccagtttaat ttgcgggacg atcgcggcca gcctgtgccg tattttattc gccatgcccg
     3301 tgagatcgat ccaggcctaa tcgatcggca aattgttcat tatggcaatt atgatccctt
     3361 tatggagttc gacatccaga tcaaccagat tgtcccttct atgggctatc gcacgcttta
     3421 tattgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg aagggattct
     3481 ggaaaatgct ttctggcaga ttgcgctcaa tgaggatggt tctctgcaac tggtagataa
     3541 agacagcggt gtgcgctatg accgggtatt gcaaattgaa gaaagctctg atgatggtga
     3601 tgaatatgac tattcacccg caaaagaaga gtgggtaatt accgcggcga acgcgaaacc
     3661 gcaatgcgat attattcatg aagcctggca gagcagggct gttatccgtt atgaaatggc
     3721 ggtgccgcgt aatctgcaag aacgcagtgc aagacaatcc actggcagag taggggtgga
     3781 gatggttgtc actctgagtc ataacagcag acgtattgat gtggatatca accttgataa
     3841 ccaggctgac gatcatcgcc tttgcgtcct gatccctaca ccttttaaca ccgacagtgt
     3901 tctggcagat acgcagtttg gttcgctaac gcgccccgtg aacgacagtg caatgaacaa
     3961 ctggcagcaa gaaggctgga aagaagcgcc ggttccggta tggaatatgc tcaactatgt
     4021 tgccttacag gaagggcgta acggcatggc tgtctttagc gaagggctac gtgaatttga
     4081 agtcatcggt gaagagaaga aaacctttgc cattacgttg ctgcgtggcg tgggcttact
     4141 gggcaaagaa gatctgcttt taaggcctgg gcggccttcg ggaattaaaa tgccagtccc
     4201 ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt ttattgagtt ataccggtac
     4261 gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg ctgactccag tacagtgtta
     4321 caacaaaatc ccatgggatg cgatgaagct caacaaagcc ggattcaacg tgccggaaag
     4381 ttatagtttg ttgaaaatgc ccccagtggg atgcctgata agcgcactta agaaagctga
     4441 agaccgacaa gaagtgattt tacggctgtt taatccggct gaatcagcaa cctgtgatgc
     4501 gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa acgatgatgg atgaacacat
     4561 taccaccgag gaaaatcaag gttcaaatct atcggggcct tttttacccg gccagtcacg
     4621 gacgttcagt taccggcttg cctgaatagc aatcaaaccg aagccacata tgcgcggcca
     4681 gattgttgac aaagggcgct ttgttcatgc cggatacggc atgaacgctt tattcggtct
     4741 acaaaagcag gcaaattcaa tatattgcag agatgatgta gacactggca agcgtagcgc
     4801 atcaggtaat tttgcgttta tcttcactct caagccacgt atatgtggct ttatttttaa
     4861 caaaat
//
LOCUS       DAZGTR010000225         4777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGTR010000225 DAZGTR010000000
VERSION     DAZGTR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.227"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            159..584
                     /locus_tag="ACHLCY_004809"
     CDS             159..584
                     /locus_tag="ACHLCY_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0371887.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            631..1053
                     /locus_tag="ACHLCY_004810"
     CDS             631..1053
                     /locus_tag="ACHLCY_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0371888.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGETMS"
     gene            1050..1241
                     /locus_tag="ACHLCY_004811"
     CDS             1050..1241
                     /locus_tag="ACHLCY_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371889.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            2279..2509
                     /locus_tag="ACHLCY_004812"
     CDS             2279..2509
                     /locus_tag="ACHLCY_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371890.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            2561..3922
                     /locus_tag="ACHLCY_004813"
     CDS             2561..3922
                     /locus_tag="ACHLCY_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP0371891.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVKRLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDRYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAETEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            3969..4531
                     /locus_tag="ACHLCY_004814"
                     /pseudo
     CDS             3969..4531
                     /locus_tag="ACHLCY_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            complement(4617..>4777)
                     /locus_tag="ACHLCY_004815"
     CDS             complement(4617..>4777)
                     /locus_tag="ACHLCY_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371892.1"
                     /translation="RKKKSSSSSSSSSSSSSSSSSRNNNIISNRQMTGLKRQKPPSER
                     PRNERGGA"
BASE COUNT         1037 a         1314 c         1431 g          995 t
ORIGIN      
        1 cgctcagatt tcgcggtctc accctccaac tcccgtcagc cgtgtgcgtc aggctgcggc
       61 ttcccttgcg cccttgcatc cccgccttat cgcccggctt ttctggaggc acggcaccgc
      121 ccggtgtcgc ggaatatgtg aacaacggag gaaatcccat gcagtatgcg aaacctgtca
      181 ctctgaacgt tgaagagtgc gaccgtctgt cttttctgcc ttacctgttt ggcctggatt
      241 ttctgtatgc cgaagcgtct gtatacgcgc tggcgaaaaa aatgatgccg gaatatgaag
      301 gcggattctg gcacttcatc cgcctgccgg acggtggcgg ttacatgatg ccggatggtg
      361 accgtttcca catggtgaac ggtgcaaact ggtttgaccg cacggtcagt gccgatgccg
      421 caggcatcat ccttacctcc cttgtgatta accgccagtt gtggctgtac cacgacagcg
      481 gggatgcagg gctgactcac ctgtacagga tgcgcgatgc gcagttgtgg agccacatcg
      541 aatttcaccc tgaatgcaac gcgatttacg cggcgctgga ctgattaacg gacggggcgg
      601 caatgccgcc cctgtaaagg agacgagaac atgtactgta ctgttaaaga aatcatccgc
      661 gatgtactgg atacagacgt gccggacagt gaatgcgttt ttgccgtggt gctgacccgt
      721 ggggatgtgc gtcacatagc ccaggactgg aacctgtcag acgatgagct ggaaaccgtc
      781 atgcagcggc tggacgatgc ctttgagcat ggtgcggatg tcagcgttgt tcacgacgtt
      841 gttcgtgagc tgatggagga aaagcacgcc agccgtcagg tgacagtccc ggcggtgatg
      901 ctggaaaaag tgatggcgct ggcaggcagt gaaatgaagc gcctgtatgc cgtcgggagt
      961 gagaacgggg gcgacggtga cgcattcgtc agggaggaac gcgaagcaat ggacgttgtg
     1021 ttgcaggcgc tggacgggga gacgatgtca tgaatatcag cacggaaacc cgcgaaattc
     1081 tgcgtaatta cagggccgtg attaatgcgc ggcgtcgtga aatggggcag aaaccgctca
     1141 ccaccgcgca gattgttgat gaaatctgcg attttgtggc gaatcagcag gcggttttcc
     1201 tgggcggtca ctacatctta cagggcagca gaaacaggtg atacaggggc aggcggtgga
     1261 ggccgcctgt cagcctgagc gaaaacccct ggcaggctga cgcctgccgg aagggaagcg
     1321 ggccacgctg gcgcgtcgcc cgttaccctt cccgtctgtg atgtgtctga ctcaggagtg
     1381 tttctttctt atgttatgga actgttcctg atgttgttgt tgttccttta tctgctgctc
     1441 tgattctgac tactgcagtg ttgttctgtc tgccggacgc tccttcggct cccggctgcc
     1501 tggcggcatc cggtcgtcag gcccgactcc gacaggcagc tgctggcgca cctgccagtc
     1561 tccgccgaac ccgctggcgc ggtttcgggc tgtcgctacg gtccgatgcg tgaaaacccc
     1621 gggcttgcag cgaccgttcc ggtcgcgccg cctctgtcgc gccgtggcct gcgctcctgg
     1681 catcccggca aaaggccgcc gggatgtaag gcgcaacgcc atttcgcaaa ccgcgcttca
     1741 cgtcgttgcg gcgggtccct gcccgttgtt gtgtccggtg ccattcacct gtttaagccc
     1801 ctgtcgccgg gggctgatac agccacaacc tgccgcactg actgtccagg gtaaatgaac
     1861 agcccacaaa attttttacc caaaacccgg taaaaaattt tgcgttctgc ccaggacagg
     1921 tgcgtcaggc cgtggcagtg atgccccttg cggcgaaggt tctcaaacgg gtgaagttca
     1981 taccacaggg aatacagcag caggttcccg cgaaacgtca aaaccgcggc tcccgaaact
     2041 caacgggtga tgttctttaa aaatcaggat ttatttcagg ctccggcaat acgcatttat
     2101 gtgttctgtc agtcatccgg ctggcaggta atgagaacgc aggaacagaa ggaggaaaac
     2161 accggaaagc gtcagatgca gatatgaaaa aagcaggtca gggacctgct taatccatgc
     2221 cgcagagact gcacgaacag tttttgcggc tccagtcaac agtcataagg atgcatttat
     2281 gacagtaagt attgtttcac cttccgcagc ggctgtaaag ccccgccgtc atccccgttt
     2341 cagacgcgaa gacatccccg cgccggaaat tgaccccgtg ctgaaagcct tcgggcgaca
     2401 catagcccgg agttttcacc gggggcgtgg tgtacacatc cccgccatga aaaacacggc
     2461 cttcgggcag gtgctccgca cactcgaact gaaacgcgct tttaactgat gaggtgagcc
     2521 gccccgcgag gggcggcaac agacggaagg agaacatttt atgacattag cagcaatcac
     2581 catgaccgcc ccggaagccg ccagccctgt gcagatgtac cgcgcgacct actcaccgga
     2641 tgacaacaaa ctgcgcctgt atgccgcgtc acgtcttgac ccggagacgt ataaaaaagt
     2701 gcatgatgcc ggttttcgct gggcacccaa acaggcgctg tttgtcgcgc cagcctggac
     2761 accgggccgg gaagacgtgc tcctctcact tgccggagag attgaggatg aagacagcac
     2821 gctcgctgaa cgtcaggaag cacgggcgga gcggtttacc ggatacagcg gaaagcgggc
     2881 cagtgaatcc gcacaggcac ttgatgaagt gaaaagactg gccgcgatga tcccgcccgg
     2941 tcagcccatt cttgtggggc atcacagcga acgccgcgcc cgtcgtgatg cgcagcgtat
     3001 tgaaaacggc atgaaacgtg ccgtgatgct ctttgaacgt gcggaatact gggaggagcg
     3061 ggcgcggtcg gcactgcttc acgcgaagta taaagaacgc ccggacgttc gctggcgtcg
     3121 tatcaaaaaa atcgaagctg atttgcgcaa ggctgaaaag accatcgcgc agtcgcagaa
     3181 atatctgacg atgtggcggg ctgaatcgct ggatctgaat atggcaaaac tcatcagcag
     3241 tcatgaccat atcagcgcct gtttcccgct ggacaggtat ccgcgcccgg cagaaaaaag
     3301 ccagtatgaa ggaagtcgat cgttatggtc ggccctggat gatgacatca tcaccacgga
     3361 gcaggcccgc gaaattgcga tccgctgtca tgaacggcag attcagcatc aacaacgctg
     3421 ggttaaccac tatcagaacc gcctgatcta tgagcgtgcc atgctggacg aaagcggcgg
     3481 cgtggttacc cggacacagg attttgagcc gggcggacag gttttcagcc ggggcgagtg
     3541 gctgaccatc atccgcgtga acaaaagcaa cggggcggtg agttcagtca caacgccgaa
     3601 ttacagtttt ctcgggtaca gcggcacgat gaaagtgaca cccgatcgca tcacggacta
     3661 caaagcacca tcggcagaag aggctgccgt cgccagtcag gccgcgaagc gtccgccggt
     3721 agtcaactat ccgggggaag gtttccggga aatgacaaag gcgcagtggg ccgccctgcc
     3781 ccgggactgt aaggccgtgc gcagtgtggc agagacagaa gaccacgggg cataccgcta
     3841 ccgccgcaca atggacaata atttccgcct ggtgaatgtg tatatcaccg acatgaaaat
     3901 cacggaaatc ccacagaaat aaggtatatc cccccgggaa atccccgggg gatgacggag
     3961 ataatgacat gcacagtcag ttaagagaac gtatccggct gatgcgcgca aggctggata
     4021 acgccgcgcc ggttgctgaa atccgggctg aatctcagct ttttgtgact cctgccccgg
     4081 tctgtgatcg cctggtgacg ctggcggaaa tcagcaaccg tgatcacatt ctggaaccct
     4141 ctgccggcac cggggccata ctgcgggcga ttcgggatac cgcgccggag gccatgtgtg
     4201 atgcggtgga gattaacagc gggctggtcc ggtatctgcg ggaaaatttt aacggtgtca
     4261 gggtacagtg tggcgacttc atggaatggc agccggtgca gtattacagc cggatcatta
     4321 tgaatccgcc gttcatccac gggcaggata tccggcatat cctgcgggct ttttccctgt
     4381 tgcgtccggg ggcgtgctgg ttgccgtctg tctgaacggg ccgcgccagc aggagaagct
     4441 gttaccgttt tctgacatcc gcgaggagct gccgcgcggc acgtttgctt ataccgatgt
     4501 cccgacgatg attattcgtt tgcgtgcctg atgacacagg gctggcggga ggataaatgc
     4561 ccgtctgtcc gcgccgggaa aagaccgcgc gccggtggcg ctgaaagggg ccgcgctcac
     4621 gcgccgcccc tttcattacg cggacgttct gatgggggct tctgtctttt cagacctgtc
     4681 atttgtctgt tactgatgat gttgttgttc ctgctactgc tactgctact gctactgcta
     4741 ctgctactgc tactgctact gctcttcttt ttcctgc
//
LOCUS       DAZGTR010000226         4775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGTR010000226 DAZGTR010000000
VERSION     DAZGTR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.228"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..93
                     /locus_tag="ACHLCY_004816"
     CDS             <1..93
                     /locus_tag="ACHLCY_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0371893.1"
                     /translation="PDDGGSCHLWLGRGVPPAVNRRRWKGDVWP"
     gene            84..662
                     /locus_tag="ACHLCY_004817"
     CDS             84..662
                     /locus_tag="ACHLCY_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0371894.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSVSQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            659..1054
                     /locus_tag="ACHLCY_004818"
     CDS             659..1054
                     /locus_tag="ACHLCY_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0371895.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEALDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSELITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            1062..1802
                     /locus_tag="ACHLCY_004819"
     CDS             1062..1802
                     /locus_tag="ACHLCY_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0371896.1"
                     /translation="MPVPNPVMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            1818..2240
                     /locus_tag="ACHLCY_004820"
     CDS             1818..2240
                     /locus_tag="ACHLCY_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0371897.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKQPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            2222..2656
                     /locus_tag="ACHLCY_004821"
     CDS             2222..2656
                     /locus_tag="ACHLCY_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0371898.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            2649..>4775
                     /locus_tag="ACHLCY_004822"
     CDS             2649..>4775
                     /locus_tag="ACHLCY_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0371899.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQECLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIG"
BASE COUNT         1117 a         1187 c         1556 g          915 t
ORIGIN      
        1 ccggatgatg gcggaagctg tcatctctgg cttgggcggg gcgtaccgcc tgccgttaac
       61 cgtcgccgct ggaaggggga tgtatggcca taaaaggtct tgagcaggcc gttgaaaacc
      121 tcagccgtat cagcagaacg gcggtgcccg gtgccgccgc aatggccatt aaccgcgttg
      181 cttcatccgc gatatcgcag tcggtgtcac aggttgcccg tgagacaaag gtacgccgga
      241 aactggtaaa ggaaagggcc aggctgaaaa gggccacggt caaaaatccg caggccagaa
      301 tcaaggttaa ccggggggat ttgcccgtaa tcaagctggg taatgcgcgg gttgtccttt
      361 cgcgccgcag gcgtcgtaaa aaggggcagc gttcatccct gaaaggtggc ggcagcgtgc
      421 ttgtggtggg taaccgtcgt attcccggcg cgtttattca gcaactgaaa aatggccggt
      481 ggcatgttat gcagcgtgtg gccgggaaaa accgttaccc cattgatgtg gtgaaaatcc
      541 cgatggcggt gccgctgacc acggcgttta aacaaaatat tgagcggata cggcgtgaac
      601 gtcttccgaa agagctgggc tatgcgctgc agcatcaact gaggatggta ataaagcgat
      661 gaaacatact gaactccgtg cagccgtact ggatgcactg gagaagcatg acaccggggc
      721 gacgtttttt gatggtcgcc ccgctgtttt tgatgaggcg gattttccgg cagttgccgt
      781 ttatctcacc ggcgctgaat acacgggcga agcgctggac agcgatacct ggcaggcgga
      841 gctgcatatc gaagttttcc tgcctgctca ggtgccggat tcagagctgg atgcgtggat
      901 ggagtcccgg atttatccgg tgatgagcga tatcccggca ctgtcagagt tgatcaccag
      961 tatggtggcc agtggctatg actaccgacg cgacgatgat gcgggcctgt ggagttcagc
     1021 cgatctgact tatgtcatta cctatgaaat gtgaggacgc tatgcctgta ccaaatcctg
     1081 taatgccggt gaaaggtgcc gggaccaccc tgtgggttta taaggggagc ggtgaccctt
     1141 atgcgaatcc gctttcagac gttgactggt cgcgtctggc aaaagttaaa gacctgacgc
     1201 ccggcgaact gaccgctgag tcctatgacg acagctatct cgatgatgaa gatgcggact
     1261 ggactgcgac cgggcagggg cagaaatcag ccggagatac cagcttcacg ctggcgtgga
     1321 tgcccggaga gcaggggcag caggcgctgc tggcgtggtt taatgaaggt gatacccgtg
     1381 cctataaaat ccgcttcccg aacggcacgg tcgatgtgtt ccgtggctgg gtcagcagta
     1441 tcggtaaagc ggtgacggcg aaggaagtga tcacccgcac ggtgaaggtc accaatgtgg
     1501 gacgcccgtc gatggcagaa gatcgcagca cggtcacagc ggcaaccggc atgaccgtga
     1561 cgcctgccag cacctcggtg gtgaaagggc agagcaccac gctgaccgtg gcattccagc
     1621 cggaaggcgc aaccgacaag agcttccgtg cggtgtctgc ggataaaaca aaagccaccg
     1681 tgtcggtcag tggtatgacc atcaccgtga aaggtgttgc tgcaggcaag gtcaacattc
     1741 cggtcgtatc cggtaatggt gaacttgctg cggttgcaga aatcactgtc accgacagtt
     1801 aatccggaga gtcagcgatg ttcctgaaaa ccgaatcatt tgaacataac ggcgtgaccg
     1861 tcacgctttc tgaactttca gccctgcagc gtattgagca tctcgccctg atgaaacggc
     1921 aggcagaaca ggcggagtca gacagcaacc ggaagtttac agtggaagac gccatcagaa
     1981 ccggtgcttt tgtggtggcg atgtccctgt ggcataacca tccgcagaag acaaagcagc
     2041 cttccatgaa tgaagccgtt aaacagattg agcaggaagt gcttaccacc tggcccacag
     2101 aggcaatttc tcatgctgaa aacgtggtgt accggctgtc cggtatgtat gagtttgtgg
     2161 tgaatgatgc ccctgaacag gcagaggacg ccgggcctgc agagcctgtt tctgcgggaa
     2221 agtgttcgac ggtgagctga gttttgccct gaaactggcg cgcgagatgg ggcgacccga
     2281 ctggcgtgcc atgcttgccg ggatgtcatc cacggagtat gccgactggc accgctttta
     2341 cagtacccat tattttcatg atgttctgct ggatatgcac ttttccgggc tgacgtacac
     2401 cgtgctcagc ctgtttttca gcgatccgga tatgcatccg ctggatttca gtctgctgaa
     2461 ccggcgtgag gctgacgaag agcctgaaga tgatgtgctg atgcagaaag cggcagggct
     2521 tgccggaggc gtccgctttg gcccggacgg gaatgaagtt atccccgctt ccccggatgt
     2581 ggcggacatg acggaggatg acgtaatgct gatgacagta tcagaaggga tcgcaggagg
     2641 agtccggtat ggctgaaccg gtaggcgatc tggtcgttga tttaagtctg gatgcggcca
     2701 gatttgacga gcagatggcc agagtcaggc gtcatttttc cggtacggaa agtgatgcga
     2761 aaaaaacagc ggcagtcgtt gaacagtcgc tgagccgaca ggcgctggct gcacagaaag
     2821 cggggatttc cgtcgggcag tataaagccg ccatgcgtat gctgcctgca cagttcaccg
     2881 acgtggccac gcagcttgca ggcgggcaaa gtccgtggct gatcctgctg caacaggggg
     2941 ggcaggtgaa ggactccttc ggcgggatga tccccatgtt cagggggctt gccggtgcga
     3001 tcaccctgcc gatggtgggg gccacctcgc tggcggtggc gaccggtgcg ctggcgtatg
     3061 cctggtatca gggcaactca accctgtccg atttcaacaa aacgctggtc ctttccggca
     3121 atcaggcggg actgacggca gatcgtatgc tggtcctgtc cagagccggg caggcggcag
     3181 ggctgacgtt taaccagacc agcgagtcac tcagcgcact ggttaaggcg ggggtaagcg
     3241 gtgaggctca gattgcgtcc atcagccaga gtgtggcgcg tttctcctct gcatccggcg
     3301 tggaggtgga caaggtcgct gaagccttcg ggaagctgac cacagacccg acgtcggggc
     3361 tgacggcgat ggcacgccag ttccataacg tgacggcgga gcagattgcg tatgttgctc
     3421 agttgcagcg ttccggcgat gaagccgggg cattgcaggc ggcgaacgag gccgcaacga
     3481 aagggtttga tgaccagacc cgccgcctga aagagaacat gggcacgctg gagacctggg
     3541 cagacaggac tgcacgggca ttcaaatcca tgtgggatgc ggtgctggat attggtcgtc
     3601 ctgataccgc gcaggagatg ctgattaagg cagaggctgc gtttaagaaa gcagacgaca
     3661 tctggaatct gcgcaaggat gattattttg ttaacgatga agcgcgggcg cgttactggg
     3721 atgatcgtga aaaggcccgt cttgcgcttg aagccgcccg aaagaaggct gagcagcaga
     3781 ctcaacagga caaaaatgcg cagcagcaga gcgataccga agcgtcacgg ctgaaatata
     3841 ccgaagaggc gcagaaggct tacgaacggc tgcagacgcc gctggagaaa tataccgccc
     3901 gtcaggaaga actgaacaag gcactgaaag acgggaaaat cctgcaggcg gattacaaca
     3961 cgctgatggc ggcggcgaaa aaggattatg aagcgacgct gaaaaagccg aaacagtccg
     4021 gcgtgaaggt gtctgcgggc gatcgtcagg aagacagtgc tcatgctgcc ctgctgacgc
     4081 ttcaggcaga actccggacg ctggagaagc atgccggagc aaatgagaaa atcagccagc
     4141 agcgccggga tttgtggaag gcggagagtc agttcgcggt actggaggag gcggcgcaac
     4201 gtcgccagct gtctgcacag gagaaatccc tgctggcgca taaagatgag acgctggagt
     4261 acaaacgcca gctggctgca cttggcgaca aggttacgta tcaggagtgc ctgaacgcgc
     4321 tggcgcagca ggcggataaa ttcgcacagc agcaacgggc aaaacgggcc gccattgatg
     4381 cgaaaagccg ggggctgact gaccggcagg cagaacggga agccacggaa cagcgcctga
     4441 aggaacagta tggcgataat ccgctggcgc tgaataacgt catgtcagag cagaaaaaga
     4501 cctgggcggc tgaagaccag cttcgcggga gctggatggc aggcctgaag tccggctgga
     4561 gtgagtggga agagagcgcc acggacagta tgtcgcaggt aaaaagtgca gccacgcaga
     4621 cctttgatgg tattgcacag aatatggcgg cgatgctgac cggcagtgag cagaactggc
     4681 gcagcttcac ccgttccgtg ctgtccatga tgacagaaat tctgcttaag caggcaatgg
     4741 tggggattgt cgggagtatc ggcagcgcca ttggc
//
LOCUS       DAZGTR010000227         4701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGTR010000227 DAZGTR010000000
VERSION     DAZGTR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.229"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..2515)
                     /gene="fimD"
                     /locus_tag="ACHLCY_004823"
     CDS             complement(<1..2515)
                     /gene="fimD"
                     /locus_tag="ACHLCY_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP0371900.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQTPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNRSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMP"
     gene            complement(2582..3307)
                     /gene="fimC"
                     /locus_tag="ACHLCY_004824"
     CDS             complement(2582..3307)
                     /gene="fimC"
                     /locus_tag="ACHLCY_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0371901.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADDVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(3344..3883)
                     /locus_tag="ACHLCY_004825"
     CDS             complement(3344..3883)
                     /locus_tag="ACHLCY_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0371902.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFIIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGLGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(3948..4496)
                     /gene="fimA"
                     /locus_tag="ACHLCY_004826"
     CDS             complement(3948..4496)
                     /gene="fimA"
                     /locus_tag="ACHLCY_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0371903.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         1184 a         1212 c         1044 g         1261 t
ORIGIN      
        1 aaggcattcc gctgaggtaa acctgaccat tatccgcaac aatgccgcta ctctggctac
       61 tctctgatgt caccatcgcc ccaaacggca gcggcttatt attgtgggtc agcgtcatga
      121 gcagttttat cccaacgcgc gctttaaact ctgctcgcac gatcgcccca cgagtgggaa
      181 caacgttagc aaccgcgtta tctaaatcga cgttatcagc cagggtattg gtatccagcg
      241 ccactctatt ttcccgatat tcagtggcat aaggcagcac ggcataacca cgccagtcgg
      301 tacgcacccc cgtctggttt tcgacttttg catcttttgc gccaggcgct ttaacaagca
      361 ccactgtttc gtttaacggc tgccccagcg ttacgccatt ggcatgagcc aataccccac
      421 cgctgactcc gtaatagagc tgcttaatat catcgctatg gctgtaaccg atattggcat
      481 tgccgtaacc accgcgataa ttcagcgtgg cgtagcctgt acttccgcta tttccatcgc
      541 ctcccccggc atagccggtt tgcacgctat agctgaggtt gttgtcttcc agcaacgtac
      601 cgtatacacc agccagattg gtcatccgac cgttgagatc gtgtgacatg ctgtagctgg
      661 cactggcatg tcgccactga gatttactgt cagaacgcag ccagtggctg aaaggaatat
      721 tgacgttaag cgctaacatc tgatcccgtc ctttttgcca ggcgtttttc gtcaggctat
      781 agctgagcgt ccagttgata tcttcgaatg cagtatttaa accagcctgg aattgctcat
      841 cgacattact cgttccccaa taagtttgat ggctaccact caaatacagt gttgatgtgc
      901 gcccgagttg ctgagtaacg gtgagctgta atttcccgcg tttgttataa gcgaggttgt
      961 aatagtcggt gaatttcggc ttaacctgaa taactccgtc ctgtgtttcg atgttgtagc
     1021 cattcattcg actgtatgtt gtatcagcga aattaaaata tccgctggtc gaataacggt
     1081 aacccactaa ctgaatattc gtgcctgact cattgagcga tttgttatag agaaaacgca
     1141 ccgattgtcc gtcatgctga ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca
     1201 cagacagagc gcccagtgcc cccatgtttt tcccgatacc gaaattaaaa gcacgataac
     1261 gatccgccag ttgcgttcca ccatatattg tccagccagc cggaaggccg tggagtaatg
     1321 tactctggaa aaagcggggt ttttcctgct gcgcatttcc actacggtat tctcctgccg
     1381 taatggaata acgagtatgc ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg
     1441 taaaaatctg cgtgctgccg tcagcctctt tgatcgttac ctgcaagtca ccactattac
     1501 ctgcggcata gatatcgttg atggtaaaag gccccggtgg caccgtacta ttataaatgt
     1561 catacccatt ttgtttaata gtgacctgtg cagtaccacg agcaataccg tggatcaccg
     1621 gggcaaatcc tctttggcta tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc
     1681 gaaagttaat accatcgaaa atatcgccct gagtataacc atcacccagc gtcagccggg
     1741 aacgtaacgg tattatgtct cgctcaagcc aggtattgat atgctgccat ttatttttgc
     1801 tacctgatga tctgtcgcta cggttataac tccaggtggt attgtcgcgt aaacgccacg
     1861 caccaatatt taacccactc tgtaggttta aatatgcata atggctgtta cccccaatcc
     1921 gattctgtac actatttccg ctgaaattat aattgagcaa tccggcatta ataccgggat
     1981 cccataactc aggaggaata taaccacgcg cgcgattact cataaatgcc tgagggatcg
     2041 tcaggttgag tcgctgctga ccaacatcta aatgcgcagt agcgtcatgg atcatggagg
     2101 ttaatggcac gcaggcatca tccgccagca gattcatacc ggagacagaa gccgtattca
     2161 gccccatact ggcaagttgc gcgcgtgtca ggcagggaac aatcccttgt tcgctgtcgc
     2221 ccgtattaaa tgtgacatca cgcgttgcca tataaccatt attcaaatag atatcgacac
     2281 gatacgtccc tggcggtaat tcttgcccat tttcaaaacg cgataaatca gccacagcct
     2341 ggggatcatc cgctaaaaag cgcggattaa aatagagttc ggcagatgac aaaggtgtct
     2401 gtgcggcaaa agcacaggcg acaaagagcc ggacaaaaaa accagccaaa cgatgcttac
     2461 gaatatgcaa gcattgtgtg tttcgctggt aaagtcttaa attcagatat gacattattc
     2521 ctccggttta aaatcacccg gcagtcaatt ctttttattc aggcgaaaaa ttccccctgc
     2581 gttattccat tacgcccgtc attttggggg taagtgcgcc ataatcattt attgttcggt
     2641 aagtaatatt gcttcccgca tcagaaggca atttaaccgt gctttcgccc attggaggca
     2701 ccaaggcatt ttcaagaacc cgggctccgg cattcaactc tgttaccgtc aggtaatagg
     2761 gtgtcgggtt aatcagcgtc agagaattcg cgctacgacg aaatcttaat ttttctgcgg
     2821 cctgatcggg tggcaacgct aatttagccg gacgatagta cagtttaatg cggctgataa
     2881 ttgcgagctg tagcgtattc tcagtcaatt ttgatttatc cattgacgga atcgctttaa
     2941 cgttcatcca gaataaactt tcccggtcct gtggcaattg gttatttgtt gcatcaagaa
     3001 tacgcaaggt attctctttt tttcccttca tcgcaaacag aggaggcgtc acgataaaac
     3061 gaccatcctt tacatcatcg gcattttcca cccatgattg aattaaatag gtactatttt
     3121 catcattatt tgtcacggca agttgcactt gtttttgccc tgccggataa attacgcgag
     3181 tcgcacctaa ggccactccc gcttcagcgc gtccggcaac catcattgcc atgaacatca
     3241 ggatacctgc cagcaagcag aatgttattt cctgcgattt ccttacattg acgtttttat
     3301 tactcactgt cctgttcctg ttatcacatt atctgctgaa caattactga taggttaaag
     3361 agaaccaggc ctgggcattg gcgatgccgc cagtaacccg acgcccggta gcacgatatt
     3421 tggcgatgaa atgtagcgaa gtagagcctg agtaaagccg tttccagttt gctggaggac
     3481 gattaatcgg tacgaggttt ccttcatcat caaacaacgc tacgccaata ttggtggcta
     3541 tccctagccc ctctcccacg gaaagcacat ccggattttt accatccgcg acaccgtgaa
     3601 acgccacacc tacacgttcg ctcaccaccg tgctacattc ccgtaaatga ataataaaag
     3661 gcaccggtgc gctatcttcc cctaccgcat gaaaccggtt actgctgatt tgccccatat
     3721 tgaccgtcat ttgtttatca ccggcttcaa tccggcaagt ttccgcaata atgacgccct
     3781 gaaattgcat atttccgccg ggcaacgtgg tattccattt atttccggcc agagcaaaca
     3841 ttggcagcaa cgacgctaat agaaataatc tcttcctttt catcgttatt tttatcgcac
     3901 aagggtgggc atccctgccc gtaatgacgt ccctgaacct gggtaggtta ttgatactga
     3961 accttgaagg tcgcatccgc attagcagca cccggggttg cggccccggt tgcaaaataa
     4021 cgcgcctgga acggaatggt attggttccg ttattcaggg ttgtttctga actaaatgtc
     4081 gcaccgtcca gcgccagcgc agcacccgtt ctgtccagga tctgcacacc aacgtttgtt
     4141 gcgctacccg cagcagaact ctgcagagcc agtacatcag tatgcgtagc atcaattgcc
     4201 gtacctaaga aggcaacagc ggcttttgtg gcaacagtgg tatcgcaatc attcagctga
     4261 atgttaaaac caacggcaga gctggttgct ccagcctgct tcaggctagc ggtacgaacc
     4321 tggcccaact gaacggtttg atcaacagag cctgcatcaa ctgcgcaagc ggcgttaaca
     4381 acttcccctt taaagtgaac ggtcccacca tttaccgtcg tagtatcggc cagagccgct
     4441 gcggaactga gggacagagc cgacagaaca acgattgcca gagttttaat tttcatgctg
     4501 ctttccttta aaaaaactat ttctaaatcg acatgggcag tcgttctgta cactttgttt
     4561 tgtcaacgag tttgactgcc aacactgcac agttttcccc caaaagatga aacatttggg
     4621 gccattttga ctcatagagg aaagcatcgc gcacaaactt tttcagttta ttcgttggct
     4681 taatattcta ttgttatctt t
//
LOCUS       DAZGTR010000228         4692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGTR010000228 DAZGTR010000000
VERSION     DAZGTR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.230"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1334
                     /locus_tag="ACHLCY_004827"
     CDS             <1..1334
                     /locus_tag="ACHLCY_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0371904.1"
                     /translation="GTILSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGT
                     SYQVAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKN
                     SLSANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYD
                     ENNKEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTRITGIAGEHD
                     QLNYGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSSGSISSGLVVWPG
                     GINITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIAN
                     SESETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQEN
                     NIGVVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1347..2306
                     /locus_tag="ACHLCY_004828"
     CDS             1347..2306
                     /locus_tag="ACHLCY_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP0371905.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGKSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(2344..3243)
                     /gene="nhaR"
                     /locus_tag="ACHLCY_004829"
     CDS             complement(2344..3243)
                     /gene="nhaR"
                     /locus_tag="ACHLCY_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP0371906.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3309..4475)
                     /gene="nhaA"
                     /locus_tag="ACHLCY_004830"
     CDS             complement(3309..4475)
                     /gene="nhaA"
                     /locus_tag="ACHLCY_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP0371907.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1416 a         1070 c          984 g         1222 t
ORIGIN      
        1 cgggtacgat tttatccgat aactataatg ctataactct gggaaatggt tggaatacac
       61 cgctgggtgc catctctttt tacgctgccc gatcgagtag taagttaaat aatgatacca
      121 gacacgaagg tacgagctat caggtcgcct ataataaata tctagtacaa acagccactc
      181 gatttagcgt ggccgcctgg cgttatgctt cgcaggatta caggacattc agcgaccatc
      241 tttacgaaaa tgataaacac tatcatcaga gtgattatga tgatttttat gatattggca
      301 gaaaaaatag cctttctgct aatattatgc aacctttatc caataatctg ggaaatgtat
      361 cattaagtgc gctttggcgg aattactggg ggcgaagtgg aaatgctaaa gattaccaat
      421 tcagttactc caataactgg caacacatta gttatacttt ctctgccagc caatcttatg
      481 atgaaaataa taaagaagag gagcgtttta atctgtttat ctccattcct ttctactggg
      541 gggatgatat tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca
      601 aagatggcta ttcctccaac aatactagaa ttactggcat agccggtgaa catgatcagt
      661 taaattatgg tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga
      721 atttaagctg gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg
      781 cctggcaaag tagtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata
      841 tcactaacca gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac
      901 atattaatgg acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga
      961 taatacctca tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag
     1021 aattgcaggg caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt
     1081 ttacaactga ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat
     1141 taacctttgg ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg
     1201 gtagtcgcct ttttattcgc gtagatgaaa ttcccactgg cataaaagtt gctctcaatg
     1261 atgaacagaa cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat
     1321 atatatgcca gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat
     1381 attcttttat tgtgcaaagt gctccctgtg cattgaccaa tgtcggtgaa caacgtggaa
     1441 catatatact caagccactg agtatgaaag gtaatctaac cgccgaaaaa attgaaaaca
     1501 ccggaaatat cattgagtgt acagacttgc aagagacaac caacgccatt aaagttcatt
     1561 ttttaacaca gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc
     1621 cctttgaatc tgacggtaaa aaaatagagc ttccagggaa gtcatcttat aatttagaag
     1681 aaatgctatc tgcgataaat ttcaccattc catattctat cgcatctgta tcagatagtt
     1741 ctaacaccgc agacattttt cctggaactc cttttccttt gttgttcgaa attgaagtga
     1801 gttcatgtac agggaataat actaattgta atagcgtccg ctttaattat aatttaaata
     1861 ctattttaca ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac
     1921 gatttgtact gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact
     1981 gcatccaggg cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg
     2041 aaccagtgac gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg
     2101 aggacggtgc gggtcaaata ggctttcaac tctccaatga tggtacgaat gaaatccagt
     2161 atggtaaaag caattactac tcttttcagc atccccatga aggaagtaat cagattcctc
     2221 tatttattcg cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag
     2281 tcaaaatcgt ggttatgtac aactaatcta tctcccccac cttaatttgg ggggaggtgc
     2341 cgattaacgc gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac
     2401 cgccgggtgc tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc
     2461 gacgcgacca atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg
     2521 ggcaacgaag attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa
     2581 ctcgccgagg atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc
     2641 taacattgaa cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacgg
     2701 tttttctggt ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga
     2761 gaacaggcct tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt
     2821 atgctgactt aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat
     2881 gggctcgcct tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc
     2941 atcagccaca ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag
     3001 catttcctgg cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc
     3061 gctgggttcg agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag
     3121 cgctcgaatc tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac
     3181 cacggaacct tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga
     3241 catgctcatt tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa
     3301 ccgtcctgtc aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc
     3361 gaagagatag aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg
     3421 ctaccaaagg ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc
     3481 cccaccgcca taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc
     3541 agcgccaacc agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg
     3601 atccccaatg gcagaatgga ggtcaagcca tccagcgtga caccttgcag tgaaacgcca
     3661 gcattagcaa atgcaaacag cggcaaaatc agatacgcca cccacgggtg taacacatgc
     3721 tccagtcgct tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca
     3781 attacccccg ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacactacg
     3841 ccaaccagaa tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc
     3901 gcaattgcta cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat
     3961 gcgatgataa tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc
     4021 agcgctaacg gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca
     4081 gtagccgccg ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga
     4141 tagagtaatg ccggaacaat catcccacca atagcggcga taactggaaa tgcggcctgg
     4201 cgtaagctgg ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg
     4261 aaaaataccg ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt
     4321 gaaccaaccc ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg
     4381 ccgctattgg ccataatcat cgccaggata gcggcaatga taagaataat gcctcccgag
     4441 gcatcactgc taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata
     4501 gatcgttttt actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga
     4561 ataactcgtt ttttacgatc cattgaagaa tttgcgccat cagcggtgag atgagttttt
     4621 gacggctgta agcgctcacg agtgacccta agcccagata atcatagctt aaggtgagga
     4681 tttctgaggg at
//
LOCUS       DAZGTR010000229         4671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGTR010000229 DAZGTR010000000
VERSION     DAZGTR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.231"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            8..766
                     /gene="paa"
                     /locus_tag="ACHLCY_004831"
     CDS             8..766
                     /gene="paa"
                     /locus_tag="ACHLCY_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFP0371908.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLDAAKLYAEKTG
                     IIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
     gene            1102..1257
                     /gene="hokD"
                     /locus_tag="ACHLCY_004832"
     CDS             1102..1257
                     /gene="hokD"
                     /locus_tag="ACHLCY_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0371909.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1478..1735
                     /locus_tag="ACHLCY_004833"
     CDS             1478..1735
                     /locus_tag="ACHLCY_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371910.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            1805..2083
                     /locus_tag="ACHLCY_004834"
     CDS             1805..2083
                     /locus_tag="ACHLCY_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371911.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGDEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            2085..3140
                     /locus_tag="ACHLCY_004835"
     CDS             2085..3140
                     /locus_tag="ACHLCY_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0371912.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGIVPAVRQPLAEDKSLLPFFSNERVIRAVGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATIILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            3141..3506
                     /locus_tag="ACHLCY_004836"
     CDS             3141..3506
                     /locus_tag="ACHLCY_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0371913.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLVIKIIAEPPDKRRRDLDNILKAPLDVLTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            3503..4192
                     /locus_tag="ACHLCY_004837"
     CDS             3503..4192
                     /locus_tag="ACHLCY_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0371914.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            4388..4463
                     /locus_tag="ACHLCY_004838"
     tRNA            4388..4463
                     /locus_tag="ACHLCY_004838"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4421..4423,aa:Ile,seq:cat)
     gene            4554..4627
                     /locus_tag="ACHLCY_004839"
     tRNA            4554..4627
                     /locus_tag="ACHLCY_004839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4588..4590,aa:Arg,seq:tct)
BASE COUNT         1238 a         1056 c         1247 g         1130 t
ORIGIN      
        1 gttttttatg aggaacataa tggcaggttt tttaatattc ctgtcttctg ctgcttatgc
       61 tgatatcaat ctgtatggtc ctggtggccc gcatacagcc ttgcttgatg cagccaaact
      121 ttatgccgaa aaaacaggta ttatagtgaa cgttcattac ggcccacaga acaaatggaa
      181 tgaagatgcc aaaaaaaatg cagatatctt gtttggcgca tcagaacaat ctgctctggc
      241 tatcattcgg gaccataaag acagcttcag tgaaaaagat attcagcctc tttatctgcg
      301 aaaaagtatt ttactggtaa agaaaggtaa tcctaaaaat atccggagta ttgacgacct
      361 gaccagacct gggattggcg taattgttaa tgatggtggt ggtaccagta atacatcagg
      421 cactggcgtc tgggaagata ttgccggacg taaagggaat atagaaactg tcgccgcaat
      481 ccgaaaaaat attattttat atgcgcccaa tagcggaact gcacgtaagg ctcttgagaa
      541 tcagcctgga gcagatgtct ggataacctg ggctgactgg gcagccagta atccagaaat
      601 tggtgatgtc gtggaaatag cgccagacta cgtgatatgg cgtgatatga acattacagt
      661 acgtcaggat gcaaatgatg aaacccgtcg atttgcagaa tggctacaaa ccgatgaagc
      721 ggcgcctgca ttcaaaaaat atggctggac caggaaaggc acttgacatc ctcgtccttc
      781 aggacgtgga ttctttttcc ggatgccgcg ccagcggcat gtaggggcag ctcacaaaac
      841 ggaaaaaatt gtacgctaag cctcgccagg tgaactgaat tcattccgat atgggaattc
      901 ccatatcggg cgaaaacggt ttgctgtaac ggcagagtta agtaggattg ctgcgggtgc
      961 ttgaggctat ctgcctcggg catgaacacc aacggcagat agataaaagc cccacccgac
     1021 tataaatcga agtgaggccc ctatatgctc gtcacatata gattgcctct tacggaccga
     1081 aaggtcaagg agaagcaggc tatgaagcag caaaaggcga tgttaatcgc cctgatcgtc
     1141 atctgtttaa ccgtcatagt gacggcactg gtaacgagga aagacctctg cgaggtacga
     1201 atccgaaccg gccagacgga ggtcgctgtc ttcacagctt acgaacctga ggagtaagag
     1261 accaggcggg ggagaaatcc ctcgccacct ctgatgtgtc aggcatcctc aacgcacccg
     1321 cacttaaccc gcttcggcgg gtttttgttt ttattttcaa cgcgtttgaa gttccggacg
     1381 gcgccggaat agaatcaaaa atacttaagt agcgcgcagg gagaagaggg atggaccccg
     1441 aacaggggag tgctatttat ctggaaggat tctgttgatg agaatcgaag aattacgtga
     1501 aatttttagt gaagatggcc tctatactgt gcgcgttgag aagggcgcta ttgtcagcca
     1561 ctgccgtatt aaatgtttac agtctcaaca aaggaagagt ggagctgcgt taattcattt
     1621 tgtggatggg cttgtgacgg atggttttat tttgcgtgca aatgaatttg tcacatcgtt
     1681 gccgtctctg aaagaagctg ggattaaggc tggtttttct gcttttgaag attagtgaat
     1741 tcatctacaa ttcagcgcag ggctgaaccc ctgttgagta acactgtgcc accggagaaa
     1801 gccgatggcg caaaattcca gactacacaa ttctgataat tcagccgtct ttgccagcag
     1861 gcacgggcgg cgttctcatg cattcaaatc tgactggttc cggcacgccc catgcactga
     1921 agaacaggcc gaatggctga ttcagaacta ccgcagacgt ggggatgagt ttaggaaagc
     1981 cctcagcctc gattatcgtc actggataat ctacgtcagg ctgccgtact ccgagcgccc
     2041 accgcgtccg tcccgcacat tccagcaacg catctggagg taacgtgcgg gtattacttc
     2101 gacctgttcc ggtaccggaa cttgggttgg tggtccttaa accaggccgt gaatccatgc
     2161 aggtattcca taaccctcga gtgcttgtgg agccggaacc gaaaagcatg cgcggtctgc
     2221 cgtccggcat cgtccctgcc gttcgccagc cgctggcgga ggataaatca ttactgccgt
     2281 ttttcagcaa tgagcgtgtg attcgtgctg ttggtggcgc tggtgcactg tctgactggc
     2341 tgttgcgtca tgtcaaatcc tgccagtggc ctcatggtga ctatcatcac agtgaaaccg
     2401 ttattcaccg ttatggtacc ggcgcaatgg tgttgtgctg gcactgcgac aaccagctgc
     2461 gtgaccagac atccgaatca ctcgagcaac ttgctcatca aaacctgtca gcatggatga
     2521 ttgacgtcat cggtcacgca ataagcggta cgcaggagcg tgaattatct ctggctgaat
     2581 tatcctggtg ggcggtccgc aatcaggtgg cggacgcgct accggaagcg gtattacgtc
     2641 gttcgctggg gttgcgtgcg gaaaaaatcc gctcaatgta ccgtgaaagc gacatcgtac
     2701 cgggagagca gaccgccacc atcatactga agcagcgcac aaaaaatctt gcgccgctgc
     2761 ctcacgccca ccagcaaaac ccgccacagg aaaagacggt ggtcagcatt gccgttgatc
     2821 cggagtcacc ggctcagtat ctccagcgcc agaaaccaca acgggaagag atgcctgtat
     2881 acacgcgctg ggtaaaaacg cagaaatgca tgacgtgcgg taatcaggca gatgatccgc
     2941 atcacatcat tggtcatgga ctgggaggga tgggaacaaa ggctgatgat ttgtttgtta
     3001 ttccgctgtg ccgtaaatgt cataacgaac tgcacgctgg ggtaaaagat tttgaagaaa
     3061 aacacggcag ccagctgttg ttgctgattc gttttttaat gcacgcgaga aattcgggtg
     3121 tcctgaagtg gaaagcatga atgactgaac ggatagaatt tgttttgcct tacccgccga
     3181 cggtgaatac ctactggcga cgtcatggca atacgtattt catctcggaa gccggaaagc
     3241 gttatcgccg tgatgtggcg ctaattgttc gccagcagcg gctgaaatta aacctgtccg
     3301 gaaggctggt gataaagatt attgcagagc caccggataa gcgccgtcgt gacctggaca
     3361 atatcctgaa agcaccactg gatgtgctga cgcatgccgg acttctcata gacgacgagc
     3421 agtttgatga aatcaatatt gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa
     3481 aaatcacaga actggagtgc gcatgaataa ccactattta cagtttgtgc gtgagcagct
     3541 cattatcgcc accgctgatt tgagtggggc aacaaaaggt cagcttgaag cctggcagga
     3601 gaatgccatg ttcgatacag ggcgttacag gcgaaaaaaa atccggtacc gcgatgaagt
     3661 gactggaaaa atgataacgc gggataatcc accaatcccg ggaaagcaat cgctggcgaa
     3721 ggtgacgtca attcctctgg tcagtccggt tgagttttcg acatcatcgt ggcggcgggc
     3781 tgttctgtct cttgaagaac atcataaagc ctggttgttg tggtgttaca gcgggagtat
     3841 ttgttgggaa tatcagatcg cgataacaca gtgggcgtgg aatgaattta atactcaatc
     3901 cggtaccaga aaaattgctg ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc
     3961 ggcgcaggca gtaaaagcag aactttttgg tggggaaggt tatgaatatc aggagctggc
     4021 attactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc actgggttgc
     4081 aatgaaacac atttttcacc gactggatag tgaggcttta ttatttgtaa tgagaacacg
     4141 ttcaaaacaa aaggcggcat tttcaaagca aagtgttgca aaagtagatt gaaaggcata
     4201 tatttcatgc aaatctgata ttttgccgat tttgtacgtg atggcaaaag caaacaaaac
     4261 ccgcccacaa gcgggttttg ttgtgccact tatctcggat agacatggtg aatgcgctgg
     4321 tggaggagat aagggtgatt tttgtatgct tgcaacattg atttcgtaac gttattatcc
     4381 tgcgcccggc cctttagctc agtggtgaga gcgagcgact cataatcgcc aggtcgctgg
     4441 ttcaaatcca gcaagggcca ccaaccgcca ctagccatca ggaaagagcg tcaacccttt
     4501 aagttgagtg tgcgaggttc gagtccccgg tggtggtcca ttatcggtat tctgcgttgt
     4561 tagctcagca ggacagagca attgccttct aagcaatcgg tcactggttc gactccagta
     4621 caacgcgcta cacttatttt cccggctcgc ttttgcgggc cttttttgta t
//
LOCUS       DAZGTR010000230         4499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGTR010000230 DAZGTR010000000
VERSION     DAZGTR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.232"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            121..261
                     /locus_tag="ACHLCY_004840"
     CDS             121..261
                     /locus_tag="ACHLCY_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP0371915.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDVLNQPGVPK
                     NG"
     gene            254..367
                     /gene="kil"
                     /locus_tag="ACHLCY_004841"
     CDS             254..367
                     /gene="kil"
                     /locus_tag="ACHLCY_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP0371916.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVEAYRKWRSK"
     gene            364..552
                     /locus_tag="ACHLCY_004842"
     CDS             364..552
                     /locus_tag="ACHLCY_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371917.1"
                     /translation="MIPVELAKTPELSRLKREYHIAEARYWRKAGDKSKKQLCLWQAQ
                     RERMNEREFLSSPSELPF"
     gene            561..1241
                     /locus_tag="ACHLCY_004843"
     CDS             561..1241
                     /locus_tag="ACHLCY_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0371918.1"
                     /translation="MGTAILILGESGTGKSTSMRNINPEEAILIKPIGKPLPFKSKNW
                     LAWDARAKKGTVVTTDKWDVIVAVIKRAHEYGKRIVIVDDFQYVMSNEFMRRSEEKSF
                     DKFTEIGRHAWEVIKAAQDAPDDLRVYFLAHTEETPMGRVKMKTIGKMLDEKITVEGM
                     FTIVLRTLTRDDQFFFTTKNNGADTVKSPMGMFDSNEIDNDLSFVDATVCDYYGINNV
                     HQIKENAA"
     gene            1238..1825
                     /locus_tag="ACHLCY_004844"
     CDS             1238..1825
                     /locus_tag="ACHLCY_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFP0371919.1"
                     /translation="MSNVIFTYNEEATLTAGQGGFINETGAYIITITEAELKQSEKGA
                     KFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGANVVHAMMGCAGIGQLTQHMVSASK
                     FVAPEFHGKKIGLVLQKVLTTNKKTGADSYQMEIRIPFIAQTGQTLKEKAEGKQPETI
                     ANMVASLKDKDNRSKNVSHNHADDYGYSQNDYPPF"
     gene            1849..2145
                     /locus_tag="ACHLCY_004845"
     CDS             1849..2145
                     /locus_tag="ACHLCY_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFP0371920.1"
                     /translation="MPAPLYGADDPRNCSGSSKAEVLGNIKNNFDAFLALTPETKAER
                     MYRRDIQLALKQEKDRTNETAMRPLRKATIDKFPEYIDPRLRNYRSRYGAISNN"
     gene            2156..2320
                     /locus_tag="ACHLCY_004846"
     CDS             2156..2320
                     /locus_tag="ACHLCY_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFP0371921.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFCSVNDNKY
                     LNAMWRSGKK"
     gene            2317..2859
                     /locus_tag="ACHLCY_004847"
     CDS             2317..2859
                     /locus_tag="ACHLCY_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0371922.1"
                     /translation="MKQMTLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALSEDQQKAIESIKQADSAVKLAHEKFSALAAENELARKAVQALCDVVGDNTEVIAEV
                     VGRDGVLVILEAMKATGNMPATDAFLAEVRAQAFNDLCSAFVKHANLAGMDDGELVRL
                     KYATDALLHCADQLRKGVHS"
     gene            3421..4044
                     /locus_tag="ACHLCY_004848"
     CDS             3421..4044
                     /locus_tag="ACHLCY_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0371923.1"
                     /translation="MTSQLIPVFNGTIDNEIALLCNARDLHAFLGVKKVFAAWITNRI
                     SEYEFIENQDYILLSNLGKQTSGRGGHNRKDYHLTLDTAKELAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYASKLYD
                     IAFETFYAVTKNRDALLREVTRLDMSSSVIQRAMPMLKSLRARQFEL"
     gene            4092..4313
                     /locus_tag="ACHLCY_004849"
     CDS             4092..4313
                     /locus_tag="ACHLCY_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0371924.1"
                     /translation="MADIIDNAAEIEELQRNFSLQKYKSDCNAPSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            4310..>4499
                     /locus_tag="ACHLCY_004850"
     CDS             4310..>4499
                     /locus_tag="ACHLCY_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0371925.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESD"
BASE COUNT         1394 a          979 c         1047 g         1079 t
ORIGIN      
        1 cgacttgttc tgattattgg aaatcttctt tgccctccag tgcgtgaggg cctttttata
       61 tgcataccaa taacgcttca ctcgaggcgt ttttcgttat gtataaataa ggagcacacc
      121 atgcaatatg ccattgcagg gtggcctgtt gctggctgcc cttccgaatc tttacttgaa
      181 cgaatcaccc gtaaattacg tgacggatgg aagcgactca tcgacgtact taatcagcca
      241 ggagttccca aaaatggata aaacacttat ggctatccag actaaattca ctatcgccac
      301 ttttattggc gatgaaaaga tgtttcgtga agccgtagaa gcctacagaa aatggaggtc
      361 aaaatgattc cggtagaact ggcgaaaacc ccagagttaa gtcgattaaa aagagagtat
      421 cacattgctg aggctcgtta ctggcgtaaa gcgggagata aatcaaagaa acaactttgc
      481 ttatggcagg cacaaagaga gcgcatgaat gagcgcgaat ttctttcctc cccatccgaa
      541 ttaccattct gaggtgaatt atgggaactg cgatattaat actcggtgag tctggcaccg
      601 gaaaatcaac cagcatgaga aatatcaatc cagaggaagc aatacttata aaaccaatag
      661 gcaagccgct accatttaaa tcaaaaaact ggcttgcatg ggatgccaga gcaaaaaaag
      721 gaaccgtagt taccactgac aaatgggacg taatagttgc tgtaattaag cgtgctcacg
      781 aatacggaaa aagaatcgtt attgttgatg acttccagta tgtgatgagc aatgagttta
      841 tgcgccgctc agaagaaaaa tcgtttgata aatttactga gataggccgc cacgcatggg
      901 aggtcattaa ggctgcacag gatgcgcctg atgacctgag agtctatttt cttgcgcaca
      961 ccgaagaaac ccctatgggg cgcgtgaaaa tgaagactat cggcaaaatg ctggacgaga
     1021 aaatcactgt cgaaggcatg tttactatag ttcttcgcac tcttacccgc gatgaccagt
     1081 tctttttcac cacgaaaaac aacggtgcag acactgttaa atccccaatg ggaatgtttg
     1141 attccaatga gattgataac gatctctctt tcgtcgatgc cactgtttgt gattactacg
     1201 gcatcaataa tgttcatcaa attaaggaaa acgccgcatg agcaacgtaa tttttactta
     1261 taacgaagaa gcaacactga ccgcagggca aggtggtttt attaacgaaa ctggcgctta
     1321 cataattacc attactgaag cagaactcaa gcaatcagaa aaaggagcca aatttattga
     1381 gttttctgga gaatccgacg acggacgtaa aatccagtat cttagcgtct gtgttcagaa
     1441 aaatgacggc acggaaaaca aatttggcgc aaatgtcgtt cacgccatga tggggtgtgc
     1501 cgggattgga caattaacgc aacatatggt ttccgccagt aaatttgttg cacctgaatt
     1561 tcatggaaag aaaatcgggt tagtgctcca gaaagtatta accacaaaca aaaagactgg
     1621 cgcagacagc taccagatgg aaatacgcat cccgtttatt gcacaaacag gtcaaaccct
     1681 taaagaaaag gcagaaggca agcaaccaga aactatcgcc aacatggttg ccagcctcaa
     1741 agataaagac aatcgctcta aaaacgtaag ccataatcat gcagatgatt atggttacag
     1801 ccagaacgat taccctcctt tctgattact gaaaataagg ctcccattat gccagcgcct
     1861 ctgtatggtg cggatgaccc gcgcaactgc tccggtagct ccaaggcgga ggtgctggga
     1921 aatatcaaaa acaatttcga cgcgtttctt gctctgacac cagaaacaaa agcagaacgg
     1981 atgtaccgac gcgatataca actcgcgcta aaacaggaga aggaccgaac aaacgaaaca
     2041 gcaatgagac cgttgcgaaa agcgacaata gacaaattcc ctgaatatat cgacccgcgc
     2101 ctgcgtaatt accgctcacg ctatggcgct atcagtaata actgaggaat ttaccatgag
     2161 aggacttgca tacaatcccg gcattcttcc ggcagaaatg attattcgcc aacgcgtaaa
     2221 gccaatgcca tcgagagagg aattgcttaa gcgaaagagt ttctgctctg ttaatgataa
     2281 caaatatctg aatgcgatgt ggcggagtgg gaagaaatga aacaaatgac actaattgag
     2341 atggatggat ttctgaaagg taaatgcatc ccatgtgatt taaaggttaa cgaaacaaac
     2401 gctgaatatc ttgtgcgtaa atttgctgaa gcggaggcca agatttcggc tctgtccgaa
     2461 gaccagcaga aagcgattga gtcaattaag caggctgatt cggctgttaa gttggcacac
     2521 gagaagtttt cggcgcttgc ggcggagaat gagctggctc gtaaggcagt tcaggcatta
     2581 tgcgatgttg ttggcgacaa caccgaggtt atcgctgagg tggttgggcg agatggcgtt
     2641 ctggttattt tggaggccat gaaggcaaca ggaaatatgc cagccaccga tgcttttctg
     2701 gctgaagtac gggcgcaggc gtttaatgac ctttgctcgg cgttcgttaa acacgcgaat
     2761 ctcgcgggaa tggatgatgg tgagctagtg aggcttaaat atgcgacgga cgccctgctg
     2821 cattgtgcag accaacttcg caagggagtg cattcatgag caacctacta ccatgtccat
     2881 tccatgaagc attaaaaaca cagaattacc gaagatcgtc caagagcata tacttttcaa
     2941 tatgttagac ctcattttaa ccatgaagaa atcgatttta caccgttctt tatacgttct
     3001 tttttgcccc aaatatgccc caaacaacca cattttgccc caatagtgcc ccaaagattt
     3061 aaggggattt aacttgatat ttcagacaat taacgcaagt ttacgatgtg aatttacgtt
     3121 aattgtctgg gtggggtata taccgtcact cgttcatcaa tattctcata gtttgacaaa
     3181 tccgccagag ctatcgcata ctgatcgcac tgaacaaata agcggtctcc gcacccgata
     3241 gctttgcggc ttttttatgc ctgcaatttg gcatagttac atccgtacaa aggtcgggtg
     3301 gagaggcgta atacaacacc cgcaagggga atatgcccag agcttcttat ttggctcagt
     3361 tgacacccga ccgccagcta ctaactggcg gttataaact aaacaaataa ggaggtcatc
     3421 atgaccagtc aactcatccc cgtattcaac ggcactatcg acaacgaaat agccctactc
     3481 tgcaatgccc gcgatctgca tgctttttta ggcgtgaaaa aggtgtttgc ggcatggatt
     3541 acaaatcgca tttcagaata cgaattcatt gaaaatcaag actatatttt gctttccaat
     3601 ttgggaaagc aaacatctgg tagaggcggt cacaaccgca aggactacca cctaactctc
     3661 gacacagcca aagaactggc gatggttgaa cgtaacgaaa aaggccgtca gatacgccga
     3721 tacttcatcg agtgcgagaa aaaacttcgc agcatgcaac cagcgcagca attcacagac
     3781 gaagaaatca tcctcctttg ctatatgcag gtacagatgg agaatgcgca ggacatctgc
     3841 aaacgtctgt acccgatatt gaaggaactt aactcatcat acgcgagtaa actgtatgac
     3901 attgcgtttg aaaccttcta tgcggtgacg aaaaacagag acgcgctact gagggaggta
     3961 acacgtctcg acatgtcaag ttccgttatc cagcgggcca tgccaatgct gaaaagcctg
     4021 cgggcaaggc aattcgaatt ataaaaccaa aggagcttcg gctccttttt tcatgcctga
     4081 aggaaaggaa aatggcagat atcatcgaca acgccgctga aatcgaagaa ttgcagcgca
     4141 atttctccct gcaaaaatac aaatccgatt gtaatgcccc atctgctact cattgttgcg
     4201 agtgtggcga tccgatagat gagcggcgac gcctggcagt tcaggggtgt cggacttgtg
     4261 caagttgcca ggaggatctg gagcttatca gtaaacagag aggttcgaag tgagcgaaat
     4321 taattatcag gcactgcgtg aaaaggcaga gaaagcaact aaaggaagct acatcgtagg
     4381 gcatacatct gttaaccagc acggcaattt aacaggagtt tttgtttgcc aaaaatggaa
     4441 aggagaaccc ggtggcgtga ttgcggaatg tcatgttaac tgcctgattg aatcagatg
//
LOCUS       DAZGTR010000231         4445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGTR010000231 DAZGTR010000000
VERSION     DAZGTR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.233"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..911
                     /locus_tag="ACHLCY_004851"
     CDS             <1..911
                     /locus_tag="ACHLCY_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0371926.1"
                     /translation="FNGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQ
                     FAAIYGLKSIDRRQEEKGYHENETSLQNYISLNKTFTIGNGFDFSLVYDLMYTDGNID
                     SPYVTGLHTVTVDNSFRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSIL
                     FQPYKRFGAWEFGIEFFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYL
                     RTRFGHGKTENADSLQYNANRTYFKTIRKATLGYEQKIGDDWIAKIEYKHAWEKEHKN
                     FYDPRDEEKYNVLNQDNIYVHAFYRF"
     gene            930..1886
                     /locus_tag="ACHLCY_004852"
     CDS             930..1886
                     /locus_tag="ACHLCY_004852"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP0371927.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSNAKAKIVVDYLNVNNLNKVLRYFKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMSDERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGDHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAQNTREYNKNLAVLNKLTKQHGVYKQ"
     gene            1977..2465
                     /locus_tag="ACHLCY_004853"
     CDS             1977..2465
                     /locus_tag="ACHLCY_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFP0371928.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLIYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            2548..3675
                     /locus_tag="ACHLCY_004854"
     CDS             2548..3675
                     /locus_tag="ACHLCY_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0371929.1"
                     /translation="MAAVILRKVEKIYDNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSLNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLDTSFCHIFDFYTEENLTNYL"
     gene            4082..>4445
                     /locus_tag="ACHLCY_004855"
     CDS             4082..>4445
                     /locus_tag="ACHLCY_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371930.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNIKILKKELSQMMFQ
                     NEREFIYNVSGFDNLHQKA"
BASE COUNT         1462 a          786 c          879 g         1318 t
ORIGIN      
        1 gctttaatgg acatgttggt actacatttg aatatgagac caaaagcact gataactatt
       61 taggtaaaac agtaaaggaa tatactaaaa cacacgagat tttccaggcc tatttcagcc
      121 aacctaattg gcaatttgca gcaatctatg gccttaagtc tatcgatcgc cggcaagaag
      181 aaaaaggcta tcacgaaaat gaaacaagtc tgcaaaacta tatatcacta aacaaaacgt
      241 tcactattgg taacggtttc gacttcagtc tggtatacga cctgatgtat accgacggaa
      301 atatagatag cccttatgtt acaggcttgc atactgtaac tgttgacaac agttttcgcc
      361 ctacgttgac gtactttaac tctgactgga acgcaggttt ttattcaaac gttgaatact
      421 tatacagtcg aaatgataag agctcctggg gcgtacgcga agaagaaggt caaagtatac
      481 tatttcagcc ctataaacgt tttggtgcat gggagtttgg tattgaattt ttctaccaga
      541 aaaagcataa cgatgaattt aatcacggta aaataaatga tcgtagtatt tataccgaaa
      601 cttactacga gccaattatc aaatatgctt ttgaaaacgc aggtaatctg tatctacgca
      661 cccgcttcgg acacgggaaa acagaaaacg cagatagttt gcaatacaat gcaaacagaa
      721 cctatttcaa aacaattcga aaagccactc taggttatga acaaaaaata ggtgacgact
      781 ggatagccaa gattgaatat aaacatgcct gggaaaaaga gcacaaaaac ttttatgatc
      841 caagagatga agaaaaatat aatgtgctca atcaggataa tatttatgtt catgctttct
      901 atcgcttttg attaaacaga gggaaaccta tgttcagcgc actagatatt aaaacgttga
      961 tgcaaggaac tttatatggt gatccatcac tcagggttga taccattcgg ccaatccata
     1021 gtcctttagt tggtggactc tcgattgtta tgacacctgg agatttgttg catataccaa
     1081 cgactggtgc ggatataatt atcggaccgg aagagatcat tagttctaat gctaaggcaa
     1141 agattgtagt cgattacctg aacgtgaata atcttaacaa agttttaaga tattttaagg
     1201 tacataagta tcgtttgttt gagcaagaaa acacttcaac gatacctgat gtatacattg
     1261 gtaaacactg ccagataggt atgaactgcc attttatgcc tggtgttaaa attatgaact
     1321 gtgtgaccat aggtgataat gttgccattc atgcgaatac agtgattaaa gaaggtacta
     1381 ttattggcaa tgacgttatc atagattcta ataactccat cggtaattat agttttgaat
     1441 atatgtcgga tgaacgtgat tcatatgtcc gagttgatag catcggtaga gtaatcatag
     1501 gtgatgatgt tgaaatcggt tgtaacaaca ccattgaccg agggacactt ggagatacga
     1561 ttataggcca aggcactcgt attgataatc aagtgcagat tggacatgac tgcataatcg
     1621 gtaataaatg cctgattgtt tcacaatgcg gtttttcagg acacgtagtt ttaggcgacc
     1681 atgttattac acatggtcaa gtcggaatcg ccggtcacat atcaattggt agttattcgg
     1741 taattaaggc taaatcaggt gtaagccatt cttgtcctga aaagagtgac ctttttggtt
     1801 accctgccca aaatacgcga gaatacaaca aaaatctcgc tgtccttaat aaactaacta
     1861 aacagcatgg tgtatataaa cagtgagtcg ttgagaagta ttatttatat acattaacat
     1921 taacactgac atagaaatag cttaactaag ctatactact tttattgaga gtttaaatga
     1981 aaaataaatt gcttattgcg gctgtgttga ctggtcttct ttcaggatgc gttgatggca
     2041 attcaccctc tggcagtcag gaaggtgtcg tgaaagcgga tcctcgctat tcagaatgta
     2101 acctccctga tgtaaatgaa agagggccaa ttgctgcggg tctttacatt tccggaaccc
     2161 tcccggaagg ctattggtta atgaagccag agcgtaaaat gcattataag ggagatggta
     2221 tttatcagct tatttattct gaaaaagcag gtgcggtaac aatgcaattt gcgactatga
     2281 actggaaccc gcagttcact gtaacaggtc ttgaattgcc attaaatcag gctaaaacac
     2341 tcaagaaagg aggcatggat aaaaatacaa aagtcgttat tcctgccgat ggaaagtatg
     2401 tttggacgat tcaaattggt cctgataaaa aggcaatagc cgccatggta gcaccatgta
     2461 aatagttatt tttagcactc cactattcaa cattcgttga atagtggagt atcagtattc
     2521 caaaattctt gttaatgaga gcagattatg gccgcggtaa ttctacgcaa agttgaaaaa
     2581 atttatgaca atggattcaa agctgttcac ggcattgatc tcgatataaa cgatggtgaa
     2641 ttcatggttt ttgttggacc atctgggtgt gccaaatcaa ctacattacg catgattgca
     2701 ggtcttgaag acgtatcagg tggtgaaatt tacattagcg accgccttgt taataccctt
     2761 ccgcctaaag atcgcagcat tgcaatggtg ttccagaact atgcgctgta tccccataag
     2821 actgtatttg acaatatggc ttttggttta aaaatgcaaa aaaggccaaa agaagagatt
     2881 aagaaaattg tcgaagaagt tgcagaaaaa cttgaaatta gcgatttact ttaccgaaaa
     2941 ccgaaagaaa tgtcgggtgg tcagcgtcag cgtgtcgcgg taggccgtgc aattgtccgt
     3001 aagccagatg ttttcttatt tgatgagcca ctatctaacc ttgatgcaaa acttcgtgtt
     3061 tcaatgcgag tcaaaattgc tcagttgcat cagtctctca aggatgaggg tcatccagca
     3121 accatgattt atgttacaca cgatcaaatt gaagcattga ccctcggtga tcgtatttgt
     3181 gtgcttaatc aggggcgaat aatgcaggtc gacacaccag ttaatctgta taaccaccct
     3241 gctaacagat tcgttgctgg ctttattggt tccccgtcaa tgaatttgat agatgcggtt
     3301 gtacgtgagg aagacggtaa actctatatt gacattgcca aaaatattcg gttgtacata
     3361 cctgagaata aacaatcagt actcaaagcg tatatagata aacccgtttg ttttggtatt
     3421 cgtccagaac atatctcttt gagtttgaat tgcaaagaaa tgaatacagt cactggtgca
     3481 ttaagtatta ttgaaaatat gggcagtgaa aaatatcttt actttaccgt taacaacaaa
     3541 gagtttattg caaaagtcaa cgatcaatca attactacag ccgatattgg taaaaatttg
     3601 tatttcaacc ttgatacaag cttctgtcat atatttgatt tttacactga agaaaatcta
     3661 actaattatt tgtgattgat atgacaataa ccccaggttg aataacggta ttaaacctta
     3721 caattccata aggcttctta ctgctcgaac tgtctattgg taaatgtcat taactccacg
     3781 aactgattct tatcaaggta attgagttac aaaattccat ccataaaaaa gagcatatat
     3841 gaataaaccg ttgataacac cgcttactca gtgagcctac tatttaggtc agagcatgtc
     3901 ttctgaccat agttggaggg cattcagatt cgcagttcac aatgcttacg tactgtagac
     3961 ctgtaaccag cttgaggatg aattacgata aatccacatt ttagctcagc aattttttcg
     4021 aaaatgttaa ttaataatgt taaaattggt aagatttcat tttcatttca ggagcgcgtc
     4081 tatgatacat ttggtaaata aattgataaa ttttggtttc actcgcaccg atgcacaggt
     4141 ttacatcagt ctgttgaaaa acggtcgtag cagtggttac aagatagcaa aagaaatttc
     4201 actttctcgt tcgtctgtat actcatctat tgataatctt tacaagaacg gttatatctt
     4261 tcttgtcgat ggtgaaacta aagaatatga agcaaaatct cctgaactca tactttcaca
     4321 aattgaaaaa agcactattg acaatataaa aattctaaaa aaagaattat cacagatgat
     4381 gtttcagaat gaacgtgaat tcatttataa tgtctctggt ttcgataact tacatcagaa
     4441 agcta
//
LOCUS       DAZGTR010000232         4434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGTR010000232 DAZGTR010000000
VERSION     DAZGTR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.234"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            188..904
                     /gene="nanC"
                     /locus_tag="ACHLCY_004856"
     CDS             188..904
                     /gene="nanC"
                     /locus_tag="ACHLCY_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP0371931.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            924..2030
                     /gene="nanM"
                     /locus_tag="ACHLCY_004857"
     CDS             924..2030
                     /gene="nanM"
                     /locus_tag="ACHLCY_004857"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0371932.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2095..3075
                     /gene="nanS"
                     /locus_tag="ACHLCY_004858"
     CDS             2095..3075
                     /gene="nanS"
                     /locus_tag="ACHLCY_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0371933.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(3083..3187)
                     /locus_tag="ACHLCY_004859"
                     /pseudo
     CDS             complement(3083..3187)
                     /locus_tag="ACHLCY_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3287..4159
                     /locus_tag="ACHLCY_004860"
     CDS             3287..4159
                     /locus_tag="ACHLCY_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP0371934.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVEKLFWDFDGKTIALPQVRKNYPF
                     EGFVEWHRKEVFRG"
BASE COUNT         1318 a          946 c         1029 g         1141 t
ORIGIN      
        1 atctctgttt atttaaataa tgtgaaaaga gatttttcac aggagacatt atacaaaaaa
       61 atataaaata cagctaccgg ttgccaaaga cactataagc ctggcaaaaa aatattacac
      121 aacataaatg ctaattgttt atgcgggctt tgtattgctt tctgtatcct acaaatgagt
      181 gaaatttatg aaaaaggcta aaatactttc tggcgtatta ttactgtgct tttcgtcccc
      241 attaatttct caggctgcga cactggacgt acgtggtgga tatcgtagtg gaagccacgc
      301 ctatgagact cgactcaaag tcagtgaggg atggcaaaat ggatggtggg caagcatgga
      361 aagtaatacc tggaatacca ttcatgataa taaaaaggaa aatgccgcac tcaatgatgt
      421 tcaggttgaa gttaattacg cgattaaact tgatgatcaa tggacggtgc gcccgggaat
      481 gttaacgcat tttagcagca acggcacacg ctacggaccc tacgtaaaac tgtcctggga
      541 cgcgacaaaa gatcttaatt ttggcattcg ctatcgttac gactggaaag cttaccgaca
      601 acaagactta tccggtgata tgtctcgtga taacgttcat cgttgggatg gatatgtcac
      661 ttaccatatt aatagtgatt tcaccttcgc atggcaaacg acgctataca gcaaacagaa
      721 cgattatcgc tatgcaaacc ataagaaatg ggcgacggaa aatgcatttg ttctacaata
      781 ccatatgacg cccgatatta cgccatacat agaatatgac tacctggacc gtcagggtgt
      841 ttacaacggc agagataatt tatcggaaaa cagttatcgc attggtgtgt catttaaact
      901 gtagtagaca ggagacagtc acaatgaata aaacaataac ggcgcttgct atcatgatgg
      961 cttcatttgc cgcaaacgcg tctgtattac ctgaaactcc tgtgccattt aaaagtggta
     1021 ccggagcaat tgataacgac actgtctaca ttggtttagg tagcgcaggt acggcatggt
     1081 acaagctgga tacacaggcc aaagataaaa aatggacagc gttagctgca ttccctggtg
     1141 gaccaagaga tcaagcaacc tcggcattta ttgatggcaa tctgtatgtg tttggcggca
     1201 ttggcaaaaa tagcaagggc ttgactcagg tatttaatga cgtacacaaa tacaacccca
     1261 aaaccaatag ttgggttaaa ttgatgtcgc atgcaccgat gggcatggcg ggtcatgtga
     1321 cttttgtaca caacggcaag gcttatgtta ctggcggtgt taaccagaat atcttcaatg
     1381 gctattttga agatctcaac gaggctggaa aagattcagc aactatagac aagatcaatg
     1441 cccattattt tgacaaaaaa gcagaagatt atttcttcaa taagtttctg ttgtcttttg
     1501 atccctcaac acagcaatgg agttacgctg gcgaatctcc ctggtacgga acggctggtg
     1561 cggcggttgt gaataaaggt gataaaacct ggcttattaa tggcgaagcc aaaccaggat
     1621 tgcgaacgga tgccgtattt gaacttgatt tcaccggtaa taatttaaaa tggaataagc
     1681 ttgctcccgt ctcatcacca gatggcgtcg ctggcggttt tgcggggata agcaatgatt
     1741 ctcttatatt tgccggaggg gccggattca aaggttcacg agaaaattac cagaacggaa
     1801 agaactatgc gcatgaaggc ctgaaaaaat catatagcac tgatattcat ctttggcata
     1861 acgggaaatg ggataaatcg ggtgaattat cgcaaggtcg ggcctacgga gtatcattgc
     1921 cctggaataa tagtctattg attattggcg gtgaaactgc aggcggcaaa gcggtgacgg
     1981 attcagtttt gatctctgtg aaggataata aagtcacagt acaaaattaa cgcttcaggg
     2041 ccccggtaaa ggggccctgc tatcaatttg tcatttcaat tacgcgaaat ttatatgaac
     2101 gcaataatat cgcccgatta ttactatgtt cttaccgttg ctggtcagtc taatgccatg
     2161 gcgtatggcg aaggactgcc attaccggac agggaagatg cgcctcatcc cagaattaaa
     2221 caattagcga gatttgcgca tacgcatccc ggaggcccgt catgtcactt taacgacatt
     2281 attccactga ctcactgccc acacgatgtt caggatatgc agggttatca ccatcctctg
     2341 gcaacgaatc atcaaacaca gtacggcacc gttggccagg cactgcatat tgcacggaaa
     2401 ttactaccct ttattcctga taatgcaggg gttctcatcg ttccatgttg ccgtggcgga
     2461 tcggctttta ccgcgggcag cgaagggaca tattcagaac ggcacggagc cagccatgat
     2521 gcttgtcgtt ggggaacgga tactccgctg taccaggatt tagtcagcag aacgcgagcc
     2581 gcactggcaa aaaatccgca gaacaaattc ctcggcgtat gctggatgca aggagaattt
     2641 gacttaatga ccagtgacta cgcgtcacac cctcaacact ttaatcatat ggttgaagcc
     2701 tttcgtaggg atctaaaaca ataccattct cagcttaata atattactga cgcgccgtgg
     2761 ttttgcggcg ataccacctg gtactggaaa gaaaatttcc cgcattcgta tgaagctatt
     2821 tatggcaatt atcaaaataa tgttttagcc aatattattt tcgtcgactt ccagcaacaa
     2881 ggtgaaagag gactgacgaa cgcgcctgat gaagatccgg acgatttaag cacgggatat
     2941 tacggttcag cgtaccggtc accggagaac tggacgacgg cactgcgaag cagtcatttc
     3001 agcacggcag cccgtcgggg ggttatttct gacaggtttg tagaagcaat tttgcagttt
     3061 tggcgcgaaa agtgagcgtt cattaattgg atttattcgc atccatcaac gcttttaact
     3121 cctccggcgt aaacggtggt ttgcgccggt gcagcatcgc atggcaatta gggtacacag
     3181 gcagcaaaaa aacaaacgag atcaatgtaa tatatcttca cttgcaggct tgaacagcag
     3241 aaaatttgcg agcatgatcg caattatttc actgccaggg atatcaatgg cttccagtaa
     3301 atcgctacag caggcaattg ccaatataaa aatctggcat aagggtgaac agcgcgcacc
     3361 gcataaacca ttgttattgc tatacgtatt agcgggatac ctgaatggac atccacgcct
     3421 tttcgattat ggatcggaaa tctacgaacc tctgcacagt ttactggaac gttttgggcc
     3481 acagcgttca cagtaccgac ctgatatgcc gttctggcga ttacaaggcg acggattctg
     3541 gcaattacac aatgccgagc tttgctcaac agcagggagc agccgacaac cgccagtgaa
     3601 ggaactgaac gaataccacg ttgcaggcgg tttcgatgaa cagcactacg ccctggtaac
     3661 cggcaataaa aaactcatca atactctggc gcaacagata ctcgaagcgc acttcaccga
     3721 gagtattcag gaagaaattg ctgacgagct gggttttgat ctccagcaaa tccgtaaaca
     3781 gcgcgacccg ttattccgca aaaatgtatt acgcgcttat aactaccagt gcgcgatatg
     3841 tggctttaat atgcgccacg acgacaccac cgtcgcgttg gaagcggcgc atataaaatg
     3901 gaaacagcaa ggaggtcctt gtgaaattcc taatggcctg gcgctttgcg caattcacca
     3961 taaagccttt gataaaggat cgattggcct ggatgaaaat atgcgggtac tggtatcaga
     4021 tgcggtaaac ggcggaggga tcgttgagaa actattctgg gattttgacg ggaaaactat
     4081 tgcgttacca caggtgcgta aaaattaccc ttttgagggg tttgtagagt ggcatcggaa
     4141 agaggttttt agggggtgag gttgataata ccgcgccccc ataatcttgc catctcgtgc
     4201 attgagatgt taaaagctac aaatgttctc cctatttttc aatgtagaca tttatagcaa
     4261 tatcaaaatt tattgttcag ctttttcaac aacaaaacca ttttgccgta caaactcaat
     4321 caacagttca atattaacaa tgccccattg attagaaata gcaacaattt caccagaagg
     4381 gagtgtaatc aacgcatctt cattgatatg atagcgactc ttttgcttct ctgg
//
LOCUS       DAZGTR010000233         4399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGTR010000233 DAZGTR010000000
VERSION     DAZGTR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.235"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..209)
                     /locus_tag="ACHLCY_004861"
     CDS             complement(<1..209)
                     /locus_tag="ACHLCY_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0371935.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHLCY_004862"
     tRNA            complement(324..399)
                     /locus_tag="ACHLCY_004862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHLCY_004863"
     tRNA            complement(406..480)
                     /locus_tag="ACHLCY_004863"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHLCY_004864"
     tRNA            complement(597..681)
                     /locus_tag="ACHLCY_004864"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHLCY_004865"
     tRNA            complement(690..765)
                     /locus_tag="ACHLCY_004865"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHLCY_004866"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHLCY_004866"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP0371936.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHLCY_004867"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHLCY_004867"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP0371937.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHLCY_004868"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHLCY_004868"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP0371938.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1125 a         1100 c          986 g         1188 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gcacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttatctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgt tcccggcgga tttgtcctgg
     4321 ttgggagagt gttcaccgac aaacatcaaa taaaacataa ggcccagtct tccgactgag
     4381 ccttatgttt tatttgatg
//
LOCUS       DAZGTR010000234         4332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGTR010000234 DAZGTR010000000
VERSION     DAZGTR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.236"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..565
                     /gene="lpfB-O113"
                     /locus_tag="ACHLCY_004869"
     CDS             <1..565
                     /gene="lpfB-O113"
                     /locus_tag="ACHLCY_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFP0371939.1"
                     /translation="ISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQ
                     EKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANI
                     RLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLP
                     ANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            590..3112
                     /gene="lpfC-O113"
                     /locus_tag="ACHLCY_004870"
     CDS             590..3112
                     /gene="lpfC-O113"
                     /locus_tag="ACHLCY_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP0371940.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3123..4195
                     /gene="lpfD-O113"
                     /locus_tag="ACHLCY_004871"
                     /pseudo
     CDS             3123..4195
                     /gene="lpfD-O113"
                     /locus_tag="ACHLCY_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system.; frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
BASE COUNT         1228 a          912 c         1012 g         1180 t
ORIGIN      
        1 tatttctgta tttagcaaag aagaaaaaat accttatctc atccaggctt gggtagatcc
       61 atttaataaa gaggataaaa gcaaagcgcc gtttaccgtt attccgccag tatcacgatt
      121 agaaccttcg caagaaaaaa tattacgcat tattcacacc aaaggtgtgt cattgcctga
      181 tgatcgcgag tcagtcttct ggctgaatat taaaaatatt ccgccatcag cgagcaacaa
      241 agcgacgaac tcgctggaaa tagcagtaaa aacgcgtatc aagctgttct ggcgaccagc
      301 aaatatccgc cttattcccg aagatgctgc gccgaaagtg aaatggcgcc gggaagggcg
      361 aaatctgata gcagagaatc ccaacccaat tcatatcagt gtgatggatg tcatcgttga
      421 tggccatgat gtgcccctca atatgatccg tccttttgaa acgctcacgt tacctttacc
      481 tgctaattct gcgggaggcc aaatgacctg gcgatttatt aatgattacg gcgcagtcag
      541 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
      601 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      661 cgaggtcagt gtattcaatc cggcgcttct ggaaatcgac catcaatccg gagtcgatat
      721 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      781 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      841 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      901 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      961 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcccca
     1021 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
     1081 taacgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
     1141 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
     1201 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
     1261 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
     1321 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
     1381 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
     1441 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt ctgccgttcc
     1501 acaaggtgct tttgagatca ccgatctcaa caccgccagt acaggtggcg atttggacgt
     1561 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1621 gattcttaaa cgtgaaggtc agacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1681 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1741 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1801 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1861 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aacgatttga
     1921 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1981 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     2041 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     2101 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     2161 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     2221 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     2281 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     2341 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     2401 acgaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     2461 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     2521 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2581 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2641 cgccgggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2701 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2761 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2821 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2881 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2941 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     3001 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcactttc
     3061 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     3121 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     3181 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     3241 ttgatttctc gggatatctt attaccgcga acgaaaataa ggttggaacg acatttaaca
     3301 ccactgtaac taatggatcc tcatatcctg gacgctgtca ttgtgatact ggtaacgtag
     3361 gagaattccc ctatatctat tatacgtcaa aaataaacca ggcattaact tatgctgggg
     3421 ttcactctaa tattaattat tatgatctta atccgaatct tgatgttggg attgctatag
     3481 atattcttgg ggttgggtat gtgaatgcac cttttgaata tcatgctaac aacccgtctg
     3541 gtaatacaaa gtacaattgc aaccgcattg aacctttaag tatatccagt ggggctaaag
     3601 cgatagtata tttttatatc aagaagacat ttgcaggaaa attgattatt cctgaaacaa
     3661 agatagtgac attgtatgga acaattagcc gtgacacccc ggtggattac tcacagccga
     3721 tggctgatgt ttatattcgg ggcgatatta cagctccgca aagttgcgaa ataaataatt
     3781 tacagccagt ttattttgat tttaaagaaa taccagctgc agatttttca tctgttgttg
     3841 gaagtgcggt aacaacacat aaattactaa aacggtcact attgagtgtg aaaacctggg
     3901 gatactgaat accgatgata tcagtacgtc tttttatgct accgaaccca atacggacaa
     3961 ttcaatggtc gtaacttcaa actctaacgt ggggataaaa atttacgaca aaaataataa
     4021 ggaaatcaaa gtgaacggtg gtgagttgcc aacagacatg ggtaaatcaa cagtgtatgg
     4081 tgaaaaatca ggtagtgtaa ctttttcagc tgctcctgcg agccttactg gagctcgccc
     4141 atccccgggg caatttactg caacagcgac gataactgtt gagattgtgc gctaataggt
     4201 ctgacaacga ttgttcatca catcgttgtc atctcaccca tttctttatc acttttaaat
     4261 gcatcatgac aaaaagatga catgtgggat tttcatcttc acatttaaaa tcagtaagtt
     4321 atttggtaat tc
//
LOCUS       DAZGTR010000235         4247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGTR010000235 DAZGTR010000000
VERSION     DAZGTR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.237"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            38..895
                     /gene="yibB"
                     /locus_tag="ACHLCY_004872"
     CDS             38..895
                     /gene="yibB"
                     /locus_tag="ACHLCY_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP0371941.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            1170..2366
                     /gene="kbl"
                     /locus_tag="ACHLCY_004873"
     CDS             1170..2366
                     /gene="kbl"
                     /locus_tag="ACHLCY_004873"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP0371942.1"
                     /translation="MRGKFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQERIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2376..3401
                     /gene="tdh"
                     /locus_tag="ACHLCY_004874"
     CDS             2376..3401
                     /gene="tdh"
                     /locus_tag="ACHLCY_004874"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP0371943.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            3652..>4247
                     /gene="waaH"
                     /locus_tag="ACHLCY_004875"
     CDS             3652..>4247
                     /gene="waaH"
                     /locus_tag="ACHLCY_004875"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0371944.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEF"
BASE COUNT         1087 a          908 c         1085 g         1167 t
ORIGIN      
        1 atataattct ttttttatat taatggtctt attagtaatg aaatcatcca ccacaattat
       61 cactgcatat tttgacattg gtagagggga ttggactgct aataaagggt tccgtgaaaa
      121 actagctcgt tcagttgatg tttattttag ttactttgag cgtctggccg cacttgaaaa
      181 tgagatgatt attttcactt ctcccgattt gaaaccaagg gttgaagcga ttcgcaacgg
      241 aaaaccaaca acggttatcg ttatcgatat aaaaaagaaa tttagatata tcagaagtcg
      301 tatcgaaaaa attcaaaaag atgagtcgtt tacaaataga cttgagcctc gacaattaaa
      361 aaacccagag tactggtcac cagagtatgt tttggtatgt aaccttaagg cctattttgt
      421 aaataaagct atcaacatgg gcttagtgaa aacgcctctt gttgcgtgga tagattttgg
      481 ctattgccgt aagccaaatg taactcgagg attgaaaatt tgggatttcc catttgatga
      541 aagtaagatg catcttttca ctataaaaaa agggcttact gtcacatctc aacaacaggt
      601 ttttgatttc atgattggca atcatgtata tattattggg ggcgcaattg ttggttcaca
      661 gcataaatgg aaagaattct ataaattagt tctggaaagt caaaaaataa cactaaataa
      721 taacattgtg gatgatgacc agggcatttt tgtcatgtgc tattacaaac gccccgatct
      781 cttcaaccta aactatctgg ggagaggaaa atggttcgat ttgtttcgct gcttcaggag
      841 taacacttta ggggcaaaaa tgcaggcgct gagaattttc ctttcaagaa aataggtttc
      901 cctccattag tgggtagcgt tttgatacca actgagatag gaaaaatgat gctttggcat
      961 cattttttct ttttatatca atgcagtaaa atcaatcatc attatgccca gccaaaactg
     1021 tacagttttt ctactatctg taggtgttga gagacatcaa gttacacgtt atttatcctg
     1081 aattttgcag aagtgttaac gcgtcatctc gtcgcgacct ataagtttgg gtaatatgtg
     1141 ctggaatttg ccctgtctgg agaatcgcaa tgcgtggaaa attttatcag cagttaacca
     1201 acgatctgga aaccgcacgg gcggaagggt tgtttaaaga agagcgcatt attacgtctg
     1261 cgcagcaagc agatatcact gtggctgatg gaagccacgt cattaacttt tgtgccaaca
     1321 actatctcgg gctggcgaat catcctgatc tgattgcggc ggcaaaggcg ggaatggatt
     1381 ctcacggttt cggcatggct tcggtgcgtt ttatttgcgg cactcaggac agccataaag
     1441 agcttgaaca aaaactggcg gccttcctgg ggatggaaga tgcgattctc tactcttcct
     1501 gctttgatgc taacggtggc ctgtttgaaa cgctgctggg cgcggaagac gccattatct
     1561 ccgacgcact gaaccacgcg tctattattg atggtgtgcg tctgtgtaaa gctaaacgct
     1621 atcgctatgc caacaacgat atgcaggagc ttgaagctcg tctgaaagaa gcgcgtgaag
     1681 ccggtgcgcg tcatgtgctg attgccaccg atggtgtatt ctcgatggac ggcgtgattg
     1741 ccaatctgaa gggcgtttgc gatctggcag ataaatacga tgccctggtg atggtagacg
     1801 actcccacgc ggtcggtttt gtcggtgaaa atggtcgtgg ttcccatgaa tactgcgatg
     1861 tgatgggccg ggtcgatatt atcaccggta cgcttggtaa agcgctgggc ggggcttctg
     1921 gtggttatac cgcggcgcgc aaagaggtgg ttgagtggct gcgccagcgt tctcgtccgt
     1981 acctgttctc caactcgctg gcaccggcca ttgttgccgc gtccatcaaa gtactggaga
     2041 tggtcgaagc gggcagcgaa ctgcgtgacc gtctgtgggc gaacgcgcgc cagttccgtg
     2101 agcaaatgtc ggcggcgggc tttaccctgg cgggagccga tcatgccatt attccggtca
     2161 tgcttggcga tgcagtagtg gcgcagaaat ttgcccgtga gctgcaaaaa gagggcattt
     2221 acgtcaccgg tttcttctat ccggtcgttc cgaaaggtca ggagcgtatt cgtacccaga
     2281 tgtctgcggc gcatacccct gagcaaatta cgcgtgcagt agaagcattc acgcgtattg
     2341 gtaaacaact gggcgttatc gcctgaggat gtgagatgaa agcgttatcc aaactgaaag
     2401 cggaagaggg catctggatg accgacgttc ctgtaccgga actcgggcat aacgatctgc
     2461 tgattaaaat ccgtaaaaca gccatctgcg ggactgacgt tcacatctat aactgggatg
     2521 agtggtcgca aaaaaccatc ccggtgccga tggtcgtagg ccatgaatat gtcggtgaag
     2581 tggtaggtat tggtcaggaa gtgaaaggct tcaaaatcgg cgatcgcgtt tctggcgaag
     2641 gccatatcac ctgcggtcac tgtcgcaact gtcgtggcgg tcgtacccat ctgtgccgca
     2701 acacgatcgg cgtcggcgtt aaccgtccgg gctgcttcgc cgaatatctg gtgatcccgg
     2761 cgttcaacgc cttcaaaatc ccagacaata tctccgacga cctggcttcc atttttgatc
     2821 ccttcggtaa cgccgtgcat accgcgctgt cgttcgatct ggtgggcgaa gatgtgctgg
     2881 tttctggtgc aggtccgata ggtattatgg ctgcggcggt ggcgaaacac gttggtgcac
     2941 gcaatgtggt gatcaccgat gttaacgaat accgccttga gctggcgcgc aaaatgggta
     3001 tcacccgtgc ggttaacgtc gcgaaagaaa accttaatga tgtgatggct gaactgggca
     3061 tgaccgaagg ctttgatgtc ggtctggaaa tgtccggcgc gccgccagcg tttcgtacta
     3121 tgctcgatac catgaaccac ggtggtcgta ttgcgatgct gggtattcca ccgtccgata
     3181 tgtctatcga ctggaccaaa gtaatcttta aaggcttgtt cattaaaggt atttacggtc
     3241 gtgagatgtt tgaaacctgg tacaagatgg cggcgctgat tcagtcaggt ctggatctct
     3301 cgccgatcat tacccatcgt ttctctatcg atgatttcca gaagggcttt gacgctatgc
     3361 gttcgggcca gtccgggaaa gtaattctga gttgggatta acacgaacaa gggctggtat
     3421 tccagccctt ttgcctgagg ataatccgtt aaatatgtaa aatcctgtca gtgtaataaa
     3481 gatatcgtaa ttgcgctgat ctcttatata gctgctctca ttatctctct accctgaagt
     3541 gactctctca cctgtaaaaa taattgtaaa aataatatct cacaggctta atagtttctt
     3601 aatacaaagc ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt tatgatgaac
     3661 agcaccaata aacttagtgt tattattccg ttatataatg cgggcgatga tttccgcact
     3721 tgtatggaat ctttaattac gcaaacctgg actgctctgg aaatcattat tattaacgat
     3781 ggttcaacgg ataattctgt tgaaatagca aagcattacg cagaaaacta tccgcacgtt
     3841 cgtttgttgc atcaggcgaa tgctggcgca tcggtagcgc gtaatcgtgg gatcgaggtg
     3901 gcgacgggca aatatgtcgc ttttgtcgat gctgacgatg aggtctatcc caccatgtac
     3961 gaaacgctga tgaccatggc gttagaggat gacctcgacg tggcgcagtg caacgctgac
     4021 tggtgttttc gtgaaacggg agaaacctgg caatccatcc ccagcgatcg ccttcgctca
     4081 accggcgtat taaccggccc ggactggctg cggatggggc tttcttcgcg ccgttggact
     4141 cacgttgtct ggatgggggt ttatcgccgt gatgttattg ttaaaaataa cattaaattt
     4201 attgccggat tacatcatca ggatattgtc tggacaacag aattcat
//
LOCUS       DAZGTR010000236         4205 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGTR010000236 DAZGTR010000000
VERSION     DAZGTR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.238"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(124..543)
                     /locus_tag="ACHLCY_004876"
     CDS             complement(124..543)
                     /locus_tag="ACHLCY_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0371945.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQIRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            623..877
                     /locus_tag="ACHLCY_004877"
     CDS             623..877
                     /locus_tag="ACHLCY_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0371946.1"
                     /translation="MNQKTLEDVIKTVRVSVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIAHASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            874..1296
                     /locus_tag="ACHLCY_004878"
     CDS             874..1296
                     /locus_tag="ACHLCY_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0371947.1"
                     /translation="MKIKHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETNAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQDALSTAIDAHDDAVQAVYRKAHFSGGGSPSDSVVVH"
     gene            1374..2162
                     /locus_tag="ACHLCY_004879"
     CDS             1374..2162
                     /locus_tag="ACHLCY_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371948.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNAECNARGVTKSAIDRITFMAGFADALIQVGWLVETNGVLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTKTDQKALPEEEKEEDINTYLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVQFRQALRKPIRTEQGANGAIRELEKFRQQGFSPEQ
                     VIRHSIANEYQGLFAPKGVRPETLLRQINTVSFPDSAIPPGFRG"
     gene            2169..2915
                     /locus_tag="ACHLCY_004880"
     CDS             2169..2915
                     /locus_tag="ACHLCY_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0371949.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRQNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELVTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIEK"
     gene            2938..3699
                     /locus_tag="ACHLCY_004881"
     CDS             2938..3699
                     /locus_tag="ACHLCY_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0371950.1"
                     /translation="METVFDALKAMGKATSVELATRLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPAPAELPEETTMATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVAETDGKAFPQ
                     PTGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLVLPSLHMANRELRRAKNH
                     VQKWERVCAALRELNKHRDIVRQIFDSSSRIVSEK"
     gene            3715..4137
                     /locus_tag="ACHLCY_004882"
     CDS             3715..4137
                     /locus_tag="ACHLCY_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0371951.1"
                     /translation="MAKVFTPEEREKIKGQVVELVRLSGRETLRALEAKTGASRYYIS
                     TLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT         1174 a          916 c         1117 g          998 t
ORIGIN      
        1 gtatataatg tactttgtgt tcatcattgt aaagtacttt aagtacattt tatgtataaa
       61 aaaatgagac gggataaagt gaagcacaaa cccggagggg gacgctaccg gatttatgct
      121 ggtttaagag gctttttgtt ttttctttcg tgctaactct tcgtaaattg cattgtactt
      181 ctgttttttc tcctcaagag tttttaaaag ttcatctgtc tcactgtcag ggagctcgtc
      241 cagaaggtca atgatgattt tttgtcttgg atttaactcc tgatagaaac gtatctgtcc
      301 actttcttct gtatcctctc ccaaaagata ggttggtgtt gttccaatga gtgttgctaa
      361 ttcccttaat ttctctcggc gaggaattgt ttcaccatta aaccatttgc taaccgcttt
      421 tggtgttaat ttcattcgac gggcaatttc tgcctgcctt ccatgttgtt cataaccagc
      481 gttttcacag gctagcgcaa gcctactggc gaactcttta cgcgctttat cttcatgaac
      541 cataagttca atgatattcg ctcttgaatg tactgtcagt tctgttatag catgtactca
      601 aagttcacat tgtgagggtg atatgaacca gaaaacactt gaagatgtaa tcaaaactgt
      661 tcgcgtttct gttgtggccg acgtttgtgg tgtcagccaa agagcaatct acaaatggat
      721 ggataacggt aaattgcctc gcacagaata taccggcgaa acaaattacg ctgaaaaaat
      781 cgctcatgca tcaaacggat tattttctgc cgatgcaatt ttaactattg gcagaaataa
      841 aactactacg aaaaagctga tgggagttga ttcatgaaaa tcaagcatga gcacatcgaa
      901 tcagtgttgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa cgcaattact
      961 gaagaatatc tgcgccaggg gggcggcgaa ttgcccctgg taccaggcaa ggactggaac
     1021 aatcagcaga atatctatca tcgttggttg aaaggtgaaa cgaatgcgca aagggaaaaa
     1081 attcagaaac tgatccctgc ggttctggca attcttcctc gcgagctgcg tcaccgactc
     1141 tgcatcttcg ataccctgga acgccgtgca ttactggcgg cacaggatgc actgagtacg
     1201 gcaattgatg cgcatgatga tgcagtccag gccgtttacc gtaaagcaca tttcagcggc
     1261 ggcggttctc ctagcgattc tgtcgtagtg cattgattga aattaatcgt accgaactgt
     1321 tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa caaatggctt
     1381 caaactggat aaagctcgaa gttattacgc cggataagcc ggaaatattc aggcttgctg
     1441 agattctgaa tattgatcca gatgccgcat tagggaaagt cattcgcttc tgggcatggg
     1501 cggatcaaca aatgatagac ggtaacgcag agtgtaacgc tcgcggcgtt acaaaaagtg
     1561 caatagatcg catcactttt atggctggtt ttgctgatgc gttaattcag gttggatggc
     1621 tggtcgaaac taatggtgtg ttgtcgcttc ctaactttga gcgccataac gggaaaagct
     1681 ctaaaaaacg ggcggttaca aacgagagag taacaaaaat acgagaactg aaacgaaaag
     1741 gtaacgctgc cagcgttaca aaaacggatc aaaaagcgtt accagaggaa gagaaagagg
     1801 aagatataaa tacttatctc cccctaaatc cccctcgcca aaaacgagcg tctaaaaaat
     1861 tcgaaccgga ggctatcgag ctgcctgact ggttgccgga aacactctgg catgagtggg
     1921 ttcagttcag gcaggcattg cgaaaaccga ttcgaacgga gcagggcgct aacggggcga
     1981 tacgggagct ggaaaaattc cgccagcagg gtttttcacc tgagcaggtg attcgacaca
     2041 gcatcgccaa tgaataccag ggcttgttcg cgccgaaagg tgttcggcca gagacgttgc
     2101 tccgacagat taacaccgtc tcgtttccgg acagtgcgat cccgccaggc ttcagggggt
     2161 aacagaccat gaaaaatatt gcgacaggcg gcgttctgga acgtatccgc agactggccc
     2221 cgccacatgt aaccgcgcca ttcaggacgg tggcggagtg gcgcgagtgg caacttgcag
     2281 aaggccagaa acgttgtgag gagatcaacc gtcagaatcg tcagttgcgg gtggaaaaaa
     2341 tcctgaatcg ctccggcata cagccgttgc accgcaaatg ctcgtttgcg aattaccagg
     2401 tgcagaacga cggccagcga tacgcgttga gtcaggcgaa atctatcgct gacgaactgg
     2461 ttaccggatg cacaaatttc gcgttcagcg gaaaacctgg taccggaaaa aaccatctgg
     2521 cggcggctat cgggaatcgc ctgctgaaag atggccagac agtgattgtg gttaccgtgg
     2581 ctgatgttat gagcgccctg cacgccagct atgacgacgg gcagtcaggc gaaaaatttt
     2641 tgcgggaact gtgcgaagtt gatctgctgg ttcttgatga aattggtatt cagcgcgaga
     2701 cgaaaaacga gcaggtggta ctgcaccaga ttgttgatcg ccggacagcg tcaatgcgca
     2761 gcgtggggat gctgacaaac ctgaactatg aggccatgaa aacattgctc ggcgagcgga
     2821 ttatggatcg catgaccatg aacggcgggc gctgggtgaa ttttaactgg gagagctggc
     2881 gcccgaatgt tggtcagcca ggtattgaga agtaattttt accgggagga aattttaatg
     2941 gagaccgttt ttgacgcact gaaagcgatg ggaaaagcca cgtcggtaga actggctacg
     3001 cgacttgata tcagtcgaga agaagtgctg aacgagctgt gggaactgaa aaaggctggc
     3061 ttcgttgata aaagcgtata cacctggcgc gtggctgata acaacgttca gcaggaacag
     3121 ccagcgccgg cagagctgcc ggaagaaacc accatggcaa cagtagcgaa aatctcagag
     3181 tgcgatttaa ccgcgacgat tgaacaacgt ggcccacaaa cggcggatga gttggctaca
     3241 ttgttcggta ccacatcacg caaagtagct tcaacgctgg caatggctat cagcaaagga
     3301 cgcctgattc gcgtaaacca gaacggtaaa tttcgttact gcataccggg cgataattta
     3361 ccagcagagc cgaaagcagc atcggtagcg gaaactgatg gtaaggcctt tcctcagccc
     3421 acaggtgttg cgttaccagt acaggaggct gcaacacagg aagatattaa aacagaaacg
     3481 gtggcggaca ttgtgcagtc gctgccatcg tttactgaaa cgcgagcgga tgacctggtt
     3541 ttaccatcgc tgcatatggc aaaccgcgaa ctgcgtcggg cgaaaaatca tgtccagaag
     3601 tgggagcgag tctgcgccgc gctgcgggag ctgaacaagc accgggatat tgttcgacag
     3661 attttcgatt cctccagtcg tattgtgtcg gaaaagtgat tgccggaggc acctatggca
     3721 aaagtattta caccagaaga gcgggaaaaa attaaagggc aggttgttga acttgtacgt
     3781 ctgagcggtc gcgagacgtt acgggctctg gaggctaaaa ccggtgcatc aaggtattac
     3841 ataagcactc tcgccagaga actggtcgcc agtggtgatg tttacaattc aggctacgga
     3901 ttattcccgt ctgagcaggc gcgtaaagac tggcaaaacg cccgcaaaaa actatcaagg
     3961 gcaaaggtga agaaaccggt tgtggttgat ccggatctta tctggtcatt accagacgga
     4021 gaaatacgcc gctacgacag gcgtctgaac ataatctgta gcgagtgccg gaagagcgaa
     4081 gctatgcagc gtgtactggc tttctatcag ggtaattttc agaaggtgct gttgtgagcc
     4141 aaattaacaa tcggaacttc gtgaagagaa agcataatcc aaatctgaat aattaagttc
     4201 agcac
//
LOCUS       DAZGTR010000237         4202 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGTR010000237 DAZGTR010000000
VERSION     DAZGTR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.239"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(200..445)
                     /locus_tag="ACHLCY_004883"
     CDS             complement(200..445)
                     /locus_tag="ACHLCY_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371952.1"
                     /translation="MKRHRLTEAEERRLAEAIAAEARAMGLTKKDKMPQETKRRLRAR
                     ILKAILDERTASKNREATLTAGQDPDDTFTWQPLRRR"
     gene            709..2802
                     /gene="cda"
                     /locus_tag="ACHLCY_004884"
     CDS             709..2802
                     /gene="cda"
                     /locus_tag="ACHLCY_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
                     /protein_id="HFP0371953.1"
                     /translation="MSDYEGSGPTEGIDYGHSMVVWPSTGLISGGDVKPRGSSGIAPS
                     MPPGWGDYSPQGIALVQSVLFPGIIRRIILDKELEEGDWSGWSVSVHSPWGNEKVSAA
                     RTVLENGLRGGLPEPSRPAAVSFARLEPASGNEQKIIRLMVTQQLEQVTDIPASQLPA
                     AGNNVPVKYRLMDLMQNGTQYMAIIGGIPMTVPVVDAVPVPDRSRPGTNIKDVYSAPV
                     SPNLPDLVLSVGQMNTPVLSNPEIQEEGVIAETGNYVEAGYTMSSNNHDVIVRFPEGS
                     GVSPLYISTVEILDSNGLSQRQEAENKAKDDFRVKKEEAVARAEAEKAKAELFSKAGV
                     NQPPVYTQEMMERANSVMNEQGALVLNNTASSVQLAMTGTGVWTAAGDIAGNISKFFS
                     NALEKVTIPEVSPLLMRISLGALWFHSEEAGAGSDIVPGRNLEAMFSLSAQMLAGQGV
                     VIEPGATSVNLPVRGQLINSNGQLALDLLKTGNESIPAAVPVLNAVRDTATGLDKITL
                     PAVVGAPSRTILVNPVPQPSVPTDTGNHQPVPVTPVHTGTEVKPVEMPVTTITPVSDV
                     GGLRDFIYWRPDAAGTGVEAVYVMLNDPLDSGRFSRKQLDKKYKHAGDFGISDTKKNR
                     ETLTKFRDAIEEHLSDKDTVEKGTYRREKGSKVYFNPNTMNVVIIKSNGEFLSGWKIN
                     PDADNGRIYLETGEL"
     gene            2799..3062
                     /locus_tag="ACHLCY_004885"
     CDS             2799..3062
                     /locus_tag="ACHLCY_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032488334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity domain-containing protein"
                     /protein_id="HFP0371954.1"
                     /translation="MNKMAMIDLAKLFLASKITAIEFSERICVERRRLYGVKDLSPNI
                     LNCGEELFMAAERFEPDADRADYEIDDNGLKVEVRSILEKFKL"
     gene            3132..3278
                     /locus_tag="ACHLCY_004886"
     CDS             3132..3278
                     /locus_tag="ACHLCY_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0371955.1"
                     /translation="MKKKIGGSMILVLAVLCLTACQANYVRDVQGGTIAPSSSSKLIG
                     VAVQ"
     gene            3985..4173
                     /locus_tag="ACHLCY_004887"
     CDS             3985..4173
                     /locus_tag="ACHLCY_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371956.1"
                     /translation="MDNTITAFEWADTARRSRTTERSESVSEEAEKRLDEHFLLTHLC
                     GISHPAWCTFHTILTDAT"
BASE COUNT         1066 a          842 c         1118 g         1176 t
ORIGIN      
        1 agtgtcatga ggtttacgca ggcaaaacca gcgttattca catggctgaa tagctatgca
       61 tacggttaaa atttatcagg tgcgatcgcg gcagcttttc gggtggtttg ttgccatttt
      121 tacctgtcag cagccgtgat cgcgctgaac gcatttcagc ggtgcgtaca ataaggggtt
      181 atgttaaata acccttagct tagcggcggc gcagtggttg ccaggtgaag gtgtcatccg
      241 ggtcctgtcc ggcagtcagt gtggcttctc gatttttgct ggccgttcgt tcatcaagaa
      301 tggctttaag tatccgtgct cggaggcgtc gctttgtttc ctgcggcatt ttgtcctttt
      361 tcgtcagccc catggcacga gcttcagcgg caatggcttc tgccagtcgg cgctcttcgg
      421 cttcggtcag tctatggcgt ttcatggtgg tctccgggtg tttctctggt gtcataacag
      481 tttaactgtt ggctctctcc ttatgctaat cagccttctt tcatgcctgt tgccattgag
      541 tttaagaatg attggttgtt tttcttgagc gttttcacaa aaacgtaaat ccagtgggtt
      601 gacaggattt acgaggtgac gtaacttttt actgtacata aaaccagtgg ttttatgtac
      661 agtttgttgt ttttaacgca ttgttttaaa agtcaaagag gtgtttttat gagtgattac
      721 gaaggtagtg gtccgacaga aggtattgat tatgggcact cgatggtcgt gtggccgtca
      781 acaggactga tttcgggtgg tgatgtgaaa ccaagaggct catcaggtat cgctccatcc
      841 atgcctccgg gatgggggga ttacagccca cagggtatcg cacttgtaca aagtgttctt
      901 tttccgggaa ttattcgccg gattattctg gataaggaac ttgaagaggg agactggtcg
      961 ggatggtctg tcagtgtgca tagcccttgg ggaaacgaga aagtttccgc tgcacgaaca
     1021 gttcttgaga atggtttacg tggtggtttg ccagaaccgt ctcgcccggc tgctgtttct
     1081 tttgcccgtc tggagcctgc ttccggaaat gagcaaaaaa ttattcgtct tatggttaca
     1141 cagcaactgg aacaggtaac ggatattccg gccagccagt taccagcagc gggtaataat
     1201 gtaccggtga aatatcgtct gatggacctt atgcagaacg gtacgcaata tatggctatt
     1261 atcggaggta ttccgatgac agtgccggta gttgatgccg ttccagttcc ggaccggagt
     1321 cgtccgggaa caaatattaa agatgtttac agtgcccctg tatccccaaa tctaccggac
     1381 ctggtattaa gtgtgggtca gatgaatact ccggttctgt ctaatcccga aattcaggaa
     1441 gaaggagtta ttgctgagac aggtaattat gttgaggctg gttatacgat gtccagtaat
     1501 aatcatgatg tcattgttcg ttttcctgaa ggcagtggtg tttctccgtt atatatttca
     1561 actgtagaga ttctggacag taatggtctg agtcagcgtc aggaagccga aaataaagca
     1621 aaggatgatt tcagagtcaa gaaagaagaa gcggtagccc gggctgaagc tgaaaaagca
     1681 aaagccgagt tatttagtaa agcaggtgtg aaccagcctc ctgtatatac acaggaaatg
     1741 atggaaaggg ctaattcagt aatgaatgag cagggagctc ttgttctgaa taacactgcc
     1801 agttctgtac aactggcgat gaccggaact ggtgtctgga ctgctgctgg tgatattgca
     1861 ggcaacataa gcaagttttt cagtaatgct ctggagaaag taaccatacc cgaagtgagt
     1921 cccctgctta tgcggatttc tcttggtgct ctgtggtttc attcagaaga agctggagca
     1981 ggaagtgata tcgtgccggg gcgaaatctg gaggcgatgt tttcgctgag tgctcagatg
     2041 ttggctggac agggcgtggt cattgaacct ggtgcgacga gtgtaaatct gcctgttcgt
     2101 ggacaattga taaacagtaa cgggcaatta gctctggatt tactgaaaac agggaatgaa
     2161 agtatcccag ctgcggttcc tgttcttaat gctgtccgtg atacagcaac aggactggat
     2221 aaaatcacgt taccagcagt agtaggcgca ccttcccgga cgattctggt taatccggta
     2281 ccacaacctt cggtgccaac agatacaggt aatcatcaac ccgttccggt tacaccagtg
     2341 catacaggaa cggaagtaaa accggtcgaa atgccagtaa cgacgattac tcctgtttct
     2401 gatgttggtg gactacgaga ttttatttac tggcgtcctg atgctgctgg gactggtgtt
     2461 gaagctgttt atgtgatgct taatgatcct ctggattcag ggcgattttc gcgtaaacaa
     2521 cttgataaaa aatataaaca tgctggtgat tttggtatta gtgatacaaa aaagaatcgt
     2581 gaaactctta ctaaattcag ggatgctatt gaggagcatt tatcggataa ggatacagta
     2641 gagaaaggaa cataccgaag agaaaaaggt tcaaaagttt attttaatcc taatacgatg
     2701 aatgtggtta taattaagtc aaatggtgag ttcttatctg ggtggaaaat aaatccagat
     2761 gcggataatg gtcgaattta tttagagaca ggtgaactat gaacaagatg gcaatgatcg
     2821 atttggcgaa attattttta gcttcgaaaa ttacagcaat tgagttttca gagcgaattt
     2881 gtgttgaacg gagaagattg tatggtgtta aggatttgtc tccgaatata ttaaattgtg
     2941 gggaagagtt gtttatggct gctgagcgat ttgagcctga tgcagatagg gctgattatg
     3001 aaattgatga taatggactt aaggttgagg tccgatctat cttggaaaaa tttaaattat
     3061 aatcatgagt gattttaata tctggcattt aagtataaat gtcagatatt cactcatgac
     3121 aaggagatgt catgaaaaaa aagatagggg gatctatgat tctcgttctt gctgttttgt
     3181 gtctgactgc ttgccaagct aactatgtaa gggatgttca gggtggaaca attgctccat
     3241 catcatcttc gaaactaatc ggggtggcgg ttcagtgaaa aatattaaag gatcttcttg
     3301 agatcctttt tttctgcgcg taatctgctg cctgtaaacg aaaaaacccg ccttgtggcg
     3361 ggtccttccg tccgatcaga agtcttcgac ctcttctaat cggcggtggt aactggcttg
     3421 gaggagcaca gtcaccaaaa ctgtcttttc agtttagcct taactggttc ataacttcaa
     3481 gagcaactcc tctaaatcag ttaccaatgg ctgctgccag tggcgttaag tcgtgtcttt
     3541 tccgggttgg ccccaagaga acagttaccg gagaaggtgc agcagtcggg ctgaacgggg
     3601 ggttcctgca tacagcccag cccggagcga actgcctaag tcgccccgag ataccagaag
     3661 acgtgagcta tgagaaagcg ccacgtgtcc cgcaagggag aaaggcggaa aaggtatccg
     3721 gtaatcgata tggcgggaac gtgggggcgc aggagggagc gattcaccgg aaacggtgat
     3781 gatctttaag ccacgtcggg tttcgccacc gctggcggat ttcatggttg agcctcacgg
     3841 ctcccacaga tgcaccgaaa aagtgtctgt tttgtgaagt ccgtcagggg ggcggagcct
     3901 atggaaaaag cgcctccggc gcggcctttt tcggggtttt cgtttcttgc tcacatgttc
     3961 tttccggcgt tatcccctga ttctgtggat aacactatca ccgcttttga gtgggcagat
     4021 accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaaaag
     4081 cgcctggacg agcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggtgc
     4141 acttttcata ccatcctcac tgatgccaca tgattaagcc agtatacact ccgctatcgc
     4201 ta
//
LOCUS       DAZGTR010000238         4170 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGTR010000238 DAZGTR010000000
VERSION     DAZGTR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.240"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            129..1787
                     /gene="dpiB"
                     /locus_tag="ACHLCY_004888"
     CDS             129..1787
                     /gene="dpiB"
                     /locus_tag="ACHLCY_004888"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP0371957.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1756..2436
                     /gene="dpiA"
                     /locus_tag="ACHLCY_004889"
                     /gene_synonym="citB"
     CDS             1756..2436
                     /gene="dpiA"
                     /locus_tag="ACHLCY_004889"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP0371958.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2477..3862)
                     /gene="dcuC"
                     /locus_tag="ACHLCY_004890"
     CDS             complement(2477..3862)
                     /gene="dcuC"
                     /locus_tag="ACHLCY_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0371959.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1156 a          963 c         1059 g          992 t
ORIGIN      
        1 caggtttaag tgatttaatt gatttaatga ataaaatttg ccacgatcat aattaatatc
       61 tatgtatttt gattcaacat tttaattaca tccgtcaaag aggctcggga caacccgcaa
      121 ggaaaacaat gttgcagctt aacgagaata aacagtttgc atttttccaa agactggcat
      181 ttccgctgcg tatctttttg ctgattctgg tgttctcaat atttgtcatt gcagccctgg
      241 cgcaatattt tacggccagt tttgaggact atttaacgct tcatgtacgc gacatggcaa
      301 tgaatcaggc gaaaattatt gcctccaatg acagtgtcat ctctgcggtg aaaacgcgtg
      361 actacaaacg gctggcgacc atcgctaaca aattacaaag agataccgat tttgattatg
      421 tggtgattgg ggaccggcac tcgatccgcc tttaccatcc taatccggag aaaattggtt
      481 atcctatgca gttcaccaaa cagggcgcgc tggagaaagg ggagagctac ttcattaccg
      541 ggaaagggtc aatggggatg gcgatgcgcg ccaaaacgcc aatctttgat gacgatggaa
      601 aagtcatcgg cgtggtgtcg attggctacc tggtgagtaa aatcgatagc tggcgggctg
      661 agtttttatt accgatggca ggcgtgtttg tcgtactgtt agggattctg atgttgctgt
      721 cgtggttcct ggcagcgcat atccgtcggc aaatgatggg catggagcca aagcaaatcg
      781 cacgcgtggt ccgtcagcaa gaggcgctgt ttagttcggt ttatgaaggg ctgattgcgg
      841 tggatccgca tggttacatt accgccatca atcgtaacgc aagaaagatg ctggggctga
      901 gctctcccgg acggcaatgg ttgggtaaac ccattgctga agtggtcagg cccgccgatt
      961 tctttaccga acagattgat gaaaaacgtc aggatgtggt ggctaacttt aacggtctga
     1021 gcgttattgc caatcgggaa gctattcgtt caggtgatga tttgctgggg gccattatca
     1081 gctttcgtag taaagacgaa atttccaccc tcaatgcgca actgacgcaa ataaaacaat
     1141 acgtcgagag ccttcgtaca ttgcgacacg agcatctcaa ttggatgtcg acgctcaatg
     1201 gtctgttgca gatgaaagag tatgatcgcg tgctggcgat ggtgcagggg gagtctcagg
     1261 cccagcaaca gcttattgac agcctgcgtg aggcgtttgc cgatcgccag gtggcggggc
     1321 tgctttttgg taaagtgcag cgcgcccggg aactggggct aaaaatgatc attgtccccg
     1381 gtagccagct ttcgcaactg ccgccaggac tggatagcac cgagtttgca gccattgtgg
     1441 gcaatttact tgataacgcc ttcgaagcca gcctgcgtag cgatgaagga aacaagatcg
     1501 ttgaattatt cctcagcgat gaaggcgatg atgtggtgat tgaagtcgcc gatcagggct
     1561 gcggcgttcc agagtctcta cgagacaaaa tatttgagca gggggtcagt acgcgtgctg
     1621 acgagcccgg tgaacatggc attgggttgt acttgattgc cagctacgta acgcgctgcg
     1681 gtggtgttat cactctcgaa gataatgatc cctgcggtac cttgttttca atctatattc
     1741 cgaaagtgaa acctaatgac agctccatta accctattga tcgttgagga cgaaacgccg
     1801 ctggcagaga tgcatgcgga atatattcgt cacattcccg gattcagtca gatattactg
     1861 gcgggaaacc tggcgcaggc ccgaatgatg atcgagcgtt ttaagccggg gctaatcttg
     1921 ctcgataact atcttcctga cggcagaggg attaatttat tgcatgaact ggtgcaggcg
     1981 cattatcccg gcgacgtggt gtttaccact gcagccagcg atatggaaac ggtgtctgaa
     2041 gccgtacgtt gtggtgtatt tgattatctc attaagccca ttgcctatga acggctgggg
     2101 caaacgctaa cccgtttccg ccagcgtaaa catatgctgg aaagtattga tagcgccagc
     2161 cagaagcaaa ttgatgagat gtttaatgcc tatgctcgcg gtgaacctaa agacgaactg
     2221 ccgaccggca ttgatccctt gacgctaaac gcggtgcgaa aactgtttaa agagcctggt
     2281 gtgcaacata cggcagaaac ggtggcgcag gcactgacca tcagtcgcac cactgccagg
     2341 cgttatcttg aatattgcgc cagccgccat ctgattattg ctgaaattgt tcacggcaaa
     2401 gttggcagac cgcaacgcat ataccacagt gggtgacatg ataaagccgg aggaaacttg
     2461 cctccggcat tactatttac ttgcctgtga ccgctgctgc cgttcctggc accatcagct
     2521 ctgtagcaac gataacaatc accaaaccaa caagcaccgg taccgaggtg cgttttacga
     2581 cttcaaacgg cgagatcttc gccatcccgg caaccgcaac gactacgcca gaaacgggcg
     2641 aaagggtacg gcccaggttt gacgcctgca gcatcgggat agtcaaatac gccgggttaa
     2701 tgccggaaga gtgcgccagt ttcgggatca tctcaacaaa cgcataaaac ggcgcattgc
     2761 ctgaaccggt cgtgactgcc gccagcatgg tcagaatcac caataccagc atcaggatga
     2821 tactcgccga accaaacgag gtagcgatag aaatcagact ttgaataaag ccaatggtgc
     2881 taagcccctg agcgaatacc ccagcggcaa ccagcagcat caccacgtta gcaaacgcat
     2941 ctgccattcc gcgataagcc acttccagac cagagaaaac tttctgggta ttaaagctgc
     3001 ggaggaactc cagaatggag gcaatcagca tacaaatcac cagaatagtg atgatgtgta
     3061 attgcggacc ccatttaccg tcaaaaatca gtacaccgat gatcggcgtg aacggcaaaa
     3121 tggcataaaa cgcaggagca gtggtggtga tttcactgac atctaacatt tcatgagaga
     3181 tgtgctcttt tttatccaga taacgttgcc agaagaagtg ggcgatcgcc atgccgataa
     3241 ttgcagcaat tgagataggc agcgtcgttt tgaaggcgaa gtcaatcagc gacatttcgg
     3301 aagcttgcgc cgccagcacc acatcccctg aagtcggtgc gagaataatc gccgccgggg
     3361 aggcacaaat ggcagcagct gcgccacgac tgataccaac gtttaccatc accggaaata
     3421 gggttgccat cagcaaaaca cccagaccgg ttgcggaaga gacggccaga gacatcagac
     3481 aggcgacaaa ataggcggca atcatcagca ggtaagggga gttaatatac tgcaatggtt
     3541 ttgacgccag cttgaccacc atatcattcg cgccgatatg ggtcatgtaa gcggcaaatc
     3601 cacacagcat cataatcatc atgccgaggt ctccgccgcg gctcattagt aatattttaa
     3661 cgtattcaac gatatccgtg gcgctgtagc ctgttgaagc ctggctggac ggtaacactt
     3721 tgtgccccat aatggcactg ataatcagca ataacaggcc accgacaaat aacacaccag
     3781 tggcggaata ccctttaatg atgtagcgag ctacacccac aataaccaca accccaataa
     3841 ggagttcaat gaatgtcagc ataatttttc ctgtctccag gccccaaagt aaataataaa
     3901 aaattcctaa agcttaagga aaaaatatgc ccaataaatt ggcgatgaat gctgattaaa
     3961 atcaagaaaa actgccatta agacattgaa gttgctgttt ttatacatag ataacaaaac
     4021 gcatatttag cggttggcaa ttatcacata ataatgtgtg taaattgatg gtggattgat
     4081 gcaaatttgt taattgcaat ggtgttagct gcggctgtgc gctcaaaaaa taatctaaca
     4141 tgagcatagg ttgacgattt atattatata
//
LOCUS       DAZGTR010000239         4127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGTR010000239 DAZGTR010000000
VERSION     DAZGTR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.241"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            227..835
                     /locus_tag="ACHLCY_004891"
     CDS             227..835
                     /locus_tag="ACHLCY_004891"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP0371960.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="ACHLCY_004892"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="ACHLCY_004892"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP0371961.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYCQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4127
                     /gene="selB"
                     /locus_tag="ACHLCY_004893"
     CDS             2321..>4127
                     /gene="selB"
                     /locus_tag="ACHLCY_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP0371962.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGND"
BASE COUNT          927 a         1040 c         1215 g          945 t
ORIGIN      
        1 gctcattctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgttggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggctggtg tgtcgatcgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaacgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgctgga cgcggacatc
     1321 gcgatcgtgc gctggcgcag ttgctgtgcc gcattacggg ggcggaagat gcctgtatcg
     1381 ttaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtggtttc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attattgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg cgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc cccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgttcccggt catgaaaaat ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 tgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggtg ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg
     2701 tcaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcgaggaa ttgatgccct gcgcgagcat ctgcttcagt taccagaacg
     2821 tgaacacgcc agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 atggctaact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc
     3001 aacagaaacc gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgcg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacgcc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgctgc gcgatatctc
     3301 ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaatt gctgcaacag cctggttata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc ccgctggcag cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctggtcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct ggtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatc
//
LOCUS       DAZGTR010000240         3994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGTR010000240 DAZGTR010000000
VERSION     DAZGTR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.242"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..605
                     /locus_tag="ACHLCY_004894"
     CDS             <1..605
                     /locus_tag="ACHLCY_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polymorphic toxin type 34 domain-containing
                     protein"
                     /protein_id="HFP0371963.1"
                     /translation="NEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQD
                     PIGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKALTK
                     WWNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDGKKT
                     DRCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            626..1468
                     /gene="yibA"
                     /locus_tag="ACHLCY_004895"
     CDS             626..1468
                     /gene="yibA"
                     /locus_tag="ACHLCY_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFP0371964.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINLLKDPNGDVRNWAAFAININKYDNSDIRDCFVEMLQDKNEEVRIEAIIGLSY
                     RKDKRVLSVLCDELKKNTVYDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDK
                     LKRS"
     gene            1496..2454
                     /locus_tag="ACHLCY_004896"
                     /pseudo
     CDS             1496..2454
                     /locus_tag="ACHLCY_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2466..2927
                     /gene="yibG"
                     /locus_tag="ACHLCY_004897"
     CDS             2466..2927
                     /gene="yibG"
                     /locus_tag="ACHLCY_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP0371965.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            3562..>3994
                     /locus_tag="ACHLCY_004898"
     CDS             3562..>3994
                     /locus_tag="ACHLCY_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP0371966.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKNKKIIDINSVNICS"
BASE COUNT         1307 a          708 c          889 g         1090 t
ORIGIN      
        1 tgaatgaaga gaacccgcat cagctgcagc agcttatccg cctgccgggg cagcagtatg
       61 atgaggagtc cggcctgtat tacaaccgcc accgctatta tgacccgctg caggggcggt
      121 atatcactca ggatccgatt gggctgaagg ggggatggaa tttttatcag tatccgttga
      181 atccagttac gaatacagat cctctggggt tagaagtttt tcctagacca ttccccttgc
      241 caattccatg gcccaaaagc cctgcacagc agcaagcaga tgataatgct gcaaaagcat
      301 tgacaaaatg gtggaacgat acagcatcac aaagaatatt tgactctcta atattgaata
      361 atccgggact agcattagat ataacaatga tagcttctcg tggaaatgtt gcagacacag
      421 ggataactga tcgtgtcaat gacataataa atgacagatt ctggagtgat gggaaaaaaa
      481 ccgacagatg tgacgtactt caggaactaa ttgattgtgg tgatattagt gctaaagatg
      541 caaaaagcac acagaaagcc tggaattgtc gtcactccag acagtcaaac gataaaaaaa
      601 gatagccctt gtggaggttc ctgcaatgtc aaatacatac cagaaaagaa aggcaagtaa
      661 agagtatggt ttatataatc aatgtaagaa actaaatgat gatgaattat ttcgcttact
      721 tgatgatcac aattccttga aaaggatttc atctgccaga gtattacagt taagaggtgg
      781 gcaagacgct gttagattgg caattgagtt ctgctctgat aaaaattata tccgtagaga
      841 tatcggagca tttatactcg ggcaaataaa aatttgcaaa aaatgcgaag ataatgtttt
      901 taatattttg aacaatatgg cattgaatga taagagcgct tgcgttcgag ctacggcaat
      961 cgagtcaacg gctcagcgat gcaagaaaaa cccaatttat tcacctaaaa tagtagaaca
     1021 atctcaaatt actgcttttg ataaatcgac taatgtcaga cgtgctacag catttgctat
     1081 ttctgttatc aatgataaag caacaattcc actattgatt aatctgttaa aagatccaaa
     1141 tggagatgtc agaaactggg ccgcatttgc aataaatatc aataaatatg ataatagtga
     1201 tattagggat tgttttgtgg agatgcttca ggataaaaat gaggaagtcc gtattgaagc
     1261 aataatcgga ctttcctaca gaaaagataa aagggtttta tctgttttat gcgatgagtt
     1321 aaaaaaaaat actgtttatg atgatatcat tgaggctgcg ggtgaattag gtgataaaac
     1381 gctacttcct gttttagata ctatgttgta caagtttgat gacaatgaaa ttataacttc
     1441 cgctattgat aagctgaagc gttcatagcg tgagttgcct atgcacagtg ggggattccc
     1501 gccggcacgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
     1561 gaggaaagcc gccgctggct ggcatcgtgc ggcctgaccg tggagcagat gaaaaaccag
     1621 atggacccgg tgtacacgcc ggcgcgaaaa atccacctgt accactgcga ccatcgcggc
     1681 ctgccgctgg cgctcatcag cacggaaggg gcaacagcgt ggtgcgcaga atatgatgaa
     1741 tggggcaacc tgctgagtga tgagaacccg catcatctgc agcagctcat tcgcctgccg
     1801 ggtcagcagt atgatgagga gtccggcctg tattacaacc gccaccgcta ttatgacccg
     1861 ctgcaggggc ggtatatcac tcaggatccg attgggctga aggggggatg gaatttttat
     1921 cagtatccgt tgaatccggt cataaatgta gatccgcaag gtttggttga tataaattta
     1981 taccccgaaa gtgatcttat ccattctgta gctgatgaga ttaatatccc aggcgttttc
     2041 acaatcgggg ggcatggtac ccccacatct attgaatccg caacgcgcag tatcatgaca
     2101 gctaaagatc tagcatatct aattaaattt gatgggaatt ataaagatgg gatgacagtt
     2161 tggttatttt cttgtaatac aggtaaagga caaaattcat ttgctagcca attagctaaa
     2221 gagttacata caaatgtaat aggacctgac acgctatgga cgtggtgggg gcgaggaact
     2281 aatggtaagt taaaaatgga tacagtgcta acagcaccaa cgaaccttaa ttcaaataag
     2341 gatctaatgg ctataacaac aaaagacctt ggtaattgga taacatatgg gccatctggg
     2401 caccccattt ctaatatgca aggtacgcca gaaaaaccca gtgatataag ataggttgta
     2461 gatgtatgaa agcatgcttg ttactatttt tttatttttc tcttattggt caattgcatg
     2521 gtgctgatgt gaaaataaaa gaaaacgaaa gtgtgatggg atctacagca atgacctatg
     2581 atttaagtga agaaaagctg atgaaactga agtataaatc acaacatggt gattcagagg
     2641 catcatttcg actatatcaa tattactgct ttactaaaaa taatattgat aaacaattgc
     2701 gattcttgga aagatcagca tctcagggga atgttacagc gcagtttaat tatggggtct
     2761 ttttatcaga tacaaatcca acattatcag aatattataa tttgaataga gctatttatt
     2821 ggatggaatt cgctgtaaat aacggtaata ttgatgcaaa gagcaaactt caagagctca
     2881 aaaagctaaa gcgaatggat agaaggaaga ataaggagaa tccgtaaaag caatagttca
     2941 tcagactgcc gcagcagtag tatgatgatg aatcagggct gtactataac tattatcggt
     3001 aacattaacc gcagcagggg cggtataaca ctcaggctcc gattgggctg gaaggtaggt
     3061 ggaattttta tcagtatccg ttgaacccga tttctggaat cgatcccctt gggattagcc
     3121 acctgtttat acagtataac tttaggtatg ctttcgtgtg tttcagatat accatgtgat
     3181 gataattttt atgatgtgct tagtattcca gtcgcttctg gtaataatat gcaatgtaaa
     3241 aataatcccc gttgtacaca tttataaata gatggccaat acctcaaggc ctatggagtt
     3301 agaatgttaa tgacccggga gcaagcaata gaaaacctga cggcatacga ttagtaccgt
     3361 cagttaatac aggaacttat aatcgcaatg gattttcaat acatagttgt ttaaacgctt
     3421 ttggtccgtc tttaggacct cgattctgtt ccgagggttg tataactggt ttatctaatg
     3481 acatgcaaaa attaaatgag ttaatatttt cagagccaga tagcacatta acagttacgg
     3541 actaaaaaat atggcaaggt tattaacaat tgtatttttt tctctatttc tgcgagtgca
     3601 gttactgcaa gacaatttca gagagagtta gatttggcag acaaagcaat attatgggca
     3661 ctaatatcag ccagtactaa agagggacga aaagcgtgtt cactaagcta ttttgcctgt
     3721 aaggccgcag aggctgaact cggactggca tatatggctg cgaatgataa caaggaattt
     3781 ctcacatcct tatctaatat aatgaggtac aaaatagatg ctggactttc cgaatcctat
     3841 acatgctatt tattaagtaa gggaaagatc ataagaccat atctgaaaaa tctaaatcca
     3901 ctccaacttg ccgctgattg cattgaaaca gtaaataaaa taaaagataa gaataaaaaa
     3961 atcattgata taaattcagt taatatttgt agtg
//
LOCUS       DAZGTR010000241         3779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGTR010000241 DAZGTR010000000
VERSION     DAZGTR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.243"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1038
                     /locus_tag="ACHLCY_004899"
     CDS             <1..1038
                     /locus_tag="ACHLCY_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0371967.1"
                     /translation="GELKTLTEIQKQELLRNATLIDQQKIREQLRSREETLKNENAAA
                     RASNDAELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQ
                     NAQYLSERLKDQAAFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISG
                     MVNNITEMLNGNKVEWRSWASSVLQEISKVLMNAAIVNGIRTAANSMSGAGGFLGSIG
                     DWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAA
                     DGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVML
                     SMRRDGGMLSG"
     gene            1031..1372
                     /locus_tag="ACHLCY_004900"
     CDS             1031..1372
                     /locus_tag="ACHLCY_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0371968.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRSTGLNPQL
                     KTFQAVFRVTDEATRCWLDEFLSWHGGYRAFLWRPPKHNRMVRVVCREWSVTDNVRYS
                     DFSCTIEQVVN"
     gene            1372..2070
                     /locus_tag="ACHLCY_004901"
     CDS             1372..2070
                     /locus_tag="ACHLCY_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0371969.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGKAVTWQGRQYQVYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(2087..2407)
                     /locus_tag="ACHLCY_004902"
     CDS             complement(2087..2407)
                     /locus_tag="ACHLCY_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0371970.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            2515..2688
                     /locus_tag="ACHLCY_004903"
     CDS             2515..2688
                     /locus_tag="ACHLCY_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP0371971.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            2759..3681
                     /locus_tag="ACHLCY_004904"
                     /pseudo
     CDS             2759..3681
                     /locus_tag="ACHLCY_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
BASE COUNT          949 a          839 c         1105 g          886 t
ORIGIN      
        1 ggtgagctga aaaccctgac ggagatacag aagcaggaac tgctgcgcaa tgcgaccctg
       61 attgaccagc aaaaaatccg ggaacagttg cgatcccggg aagagacact gaagaatgag
      121 aatgcggctg cgcgtgcgtc gaatgatgct gaactgctgg ggtacgggca gggggaacga
      181 gccagagaac gcatgcggga gttgcagcag atccgcgaca gcttccgcca gaaggatgcg
      241 gaccttcagt ctcagtatca gaccggggat atcagtgagg atttttacag acaggcacgg
      301 gcacagaacg cgcagtatct gagcgaacgc cttaaggacc aggcagcctt ttatgccgaa
      361 tcggatgtgc agcgtgcgga ctggcagaaa gggctgcagg agggattcag taactgggtg
      421 gataatgcgt ccgattacgc ctcacaggca gcacagcttg cgacggaggg tatctcaggg
      481 atggtgaata acatcacgga gatgctgaac ggaaataaag tggaatggcg cagctgggcc
      541 tcatcagtgc tacaggaaat atcaaaagtt cttatgaatg ccgcgattgt caacgggatc
      601 aggacggcgg caaacagtat gtccggtgca ggaggatttc tcggcagcat tggtgactgg
      661 ctgggcgggg cggtggccaa tgcaaaaggc ggcgtgtata cctcggcaaa cctgagtgcg
      721 tacagcaaca gtattgtgga tacgcccacg tactttgcct ttgcaaaagg ggcggggctg
      781 atgggggagg ccggtcctga agccattatg cccctgaccc gggcggcgga tggctcgctg
      841 ggtgtgcgcg cggtgggcag tatgaacggt agtgcgggtc tggtgtattc cccggtctac
      901 catatcgcca ttcagaatga cgggactaat ggccagatag ggccggaagc tgcgggcagc
      961 cttgtgcaac tgattgacca gcgggtgcag gcggtgatgt tgtccatgcg acgtgacgga
     1021 ggaatgctga gtggctgaga taaaaacgct gcatctggtc ccgcgtgaag ggatgcaggt
     1081 gagtgagaaa ccgtcggtgg cgagggtacg gtttggtgac ggttatgaac agcgccgctc
     1141 cacagggctg aatcctcaac tgaagacatt tcaggcggta ttccgggtga cggatgaggc
     1201 gacccggtgc tggctggatg aatttttatc gtggcatggt ggttaccgtg cctttttgtg
     1261 gcgaccgccg aaacataacc ggatggtcag ggtggtatgc cgggagtgga gcgtcacgga
     1321 taacgtccgg tacagtgatt tcagctgtac gatagagcag gtggtgaact gatgcaggat
     1381 attcgcgaag aaagtctgaa cgagtcggtt aagtcagagc agtcaccgcg ggtggtactc
     1441 tgggaaatcg acctgacggt acagggtggt gagcggtatt ttttctgtaa tgagctgaat
     1501 gaaaaaggga aggcggtcac ctggcagggg cggcaatatc aggtataccc gattgacggc
     1561 agcggttttg agatgaacgg gaagggcagc agtgccagac cgtcgctgac ggtgtccaat
     1621 ctgtttggtc tggtcaccgg gatggcggag gacctgcaga gcctggtggg ggccacggtg
     1681 gtccgccgcc gggtgtatgc ccgttttctg gatgcggtga attttgtggc gggcaatccg
     1741 gaagcggacc cggagcagga gctgagcgac cgctgggtgg tggagcagat gtcagagctg
     1801 acggccatga cagcctcgtt tgtgctggca acaccgacgg agacggacgg ggcgctgttt
     1861 cccggtcgca tcatgctggc gaacacctgt atgtgggatt accggggaga tgaatgcggg
     1921 tataacggtc ctgcggtggc ggatgagttc gacaacccca ccacggatat ccgtaaggac
     1981 agatgcagca agtgcatgcg cgggtgtgag atgcgcggca tggtggctaa ttttggcggt
     2041 ttcctttcca ttaataaact ttcgcagtaa tggattatgc ccaccgtcag gtgggttttt
     2101 tatttagtag ttctctcaac ttttcgttct gctctctgaa cttttcctgt atttcttttt
     2161 gcatggcgat aacctgggct tgaagttgaa ctagcgcatc aacatttagc ggaaccgaga
     2221 cgctgttaat tttatctgct atttccccga gtgtattttc tgcattcaag gcatcttcca
     2281 gtatctgaac aatctctgag ttcatagagc gcccgtttcg tttggctcgt tcagctatag
     2341 catctcgcat tccgtcagga aaacggaggt tgaacttgtc gtagtctttt acttgttttt
     2401 cggccattgc aaatctctca aaaaaaatca tggtgccata ttgccatacg atttcaatag
     2461 tggcattatg gccttcatgg cgtcactttg gcaccaaata aaggagatta gataatgcaa
     2521 gatacacttt tcactgagcg caaaaatatc aaactcaacc ttcgccttcc atcacggctg
     2581 aatgaagacc ttcgccgcct ggcggaaatg gactgtatat ctctgaactc tgcaattgtt
     2641 cgtttgctgg caaaaggtgt tagggaagag gtggcgaatg gtcgctaaaa acagcgaagc
     2701 ctcaatggct gcaaccattg aggcttctaa attaccagtt aaccacgaga aaactgatat
     2761 gactagttta gcaattgcag atcgcacaat caatgttcca ttccacggaa caaatctctt
     2821 tttggttgga attaacaatg agccttatgt tcctatgaag cctgttgttg aaggtatggg
     2881 gatggtttgg gctgctcaat ttgttaagtt aaaacagagg tttgtcaagg gtatttcgga
     2941 aatcgaaata ccttctgctg gcggtaaaca gttaatgaca tgtcttgcct ttcgtaaatt
     3001 tgcggcttgg ctttcaagca ttcaaccaaa caaagtccgc cctgaaatcc gcgacaaggt
     3061 aatccagtat caggaagagt gtgacgatgt gctctacgag tactggacta aaggccatgt
     3121 gattaacccg cgcaaagcta aaaaggcatt gccggggaaa atcaccactg aacagcagga
     3181 agccattaaa caactcgtca tgagtcgcgg tcagtctctg ccaaaagaaa aacaggcgaa
     3241 ggcgatgatc accatgtggt cgtcactgaa atctcatttt gggtgttcat acaaagaaat
     3301 cagcgaggag cagtttaccg aagctctgtc cttgctgctc gcgttccgct tgaaggagag
     3361 ttaatcggca aacaagagaa gagcaccaac gagctttccg caaaagaagc aaacagcctt
     3421 gtatggctat gggattatgc caaccgctca caggcattat tccgcgaatt gtatccagcg
     3481 ctaaagcaaa ttcaatcgaa ctattccggc agatgttaca actacggtca tgagttctcg
     3541 tatgttattg gaatggcgag agacgtttta atcaatcaca cacgagatgt tgatattaat
     3601 gagccagacg gaccaacgaa tctttccgca tgggtaaggc ttaagaacaa agaattacct
     3661 ccttcactac atcactacta acagattgcc aacgaaatga cccagcttcg gctgggtttt
     3721 ttatcaggag ttctcatgct ctatagcaat atattggcgc acgcccggcg atgtgcgcc
//
LOCUS       DAZGTR010000242         3773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGTR010000242 DAZGTR010000000
VERSION     DAZGTR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.244"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..125
                     /locus_tag="ACHLCY_004905"
     CDS             <1..125
                     /locus_tag="ACHLCY_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0371972.1"
                     /translation="DRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            531..1544
                     /gene="nleC"
                     /locus_tag="ACHLCY_004906"
     CDS             531..1544
                     /gene="nleC"
                     /locus_tag="ACHLCY_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFP0371973.1"
                     /translation="MYKTILSINMKIPVLQPSFNFFAPAGYSAAVAPNRADNAYADYV
                     LDIGKQTPLSAADLSNVYENVIRAVHDSRSRLIDQHTVDMIGNTVLDALSRSQTFRDA
                     VSYGIHNKEVYIGSIKYRNEYERNEESPVKVDDIQSLTCTELYEYDVGKEPILPICEA
                     GENENEEPYVSFSVAPDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEIL
                     ARRVAQELGWSVPDFKGYAEPEREAHLRLRNLNALRQAAMRHEKNERAFFERLGTISD
                     RYEASPDFTEYSAVTNIGYGFIQQHDFPGVAIDENLQDVNQIQLYHGAPYIFTFGDVD
                     RHN"
     gene            complement(1665..1748)
                     /locus_tag="ACHLCY_004907"
                     /pseudo
     CDS             complement(1665..1748)
                     /locus_tag="ACHLCY_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            1833..1907
                     /locus_tag="ACHLCY_004908"
                     /pseudo
     CDS             1833..1907
                     /locus_tag="ACHLCY_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(2779..3309)
                     /gene="yodB"
                     /locus_tag="ACHLCY_004909"
     CDS             complement(2779..3309)
                     /gene="yodB"
                     /locus_tag="ACHLCY_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0371974.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(3652..>3773)
                     /locus_tag="ACHLCY_004910"
     CDS             complement(3652..>3773)
                     /locus_tag="ACHLCY_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFP0371975.1"
                     /translation="HIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         1080 a          736 c          862 g         1095 t
ORIGIN      
        1 aggacagggt aatcacgcag gtggtgatac tggatgcgga taaaaagcag atacagtgcg
       61 tggtaagacc gctgcaaatt ctgcgtgctg acgggaggtg ggaaaatatt ggcggaatga
      121 aatagccgac agcttcacaa aaaccggagt ccggctccgg ttttttgtgt tgcaatgtcc
      181 gggggatatt tgttaagtaa atgcattttc atgcactgcg gttttagctg atatattaac
      241 aagccatgca aagggaaatt ttactgatat ttttgagcag tattatccat tagcatggcg
      301 ttgatgtctg ttgctacgtg atgccaatat atattgcaaa tcacagagca ggcagctcat
      361 taaacttggt aagccttgct acgataagga aggtttacca ttgtggtgtc attagctcac
      421 atgtgtatgg gagctttaaa cgttcctgtt actcgttgga acacctgcct tgcagggata
      481 aaagctatgc tgtccgttat cgtctgggct agtgaattgg tggcactgaa atatataaaa
      541 ccatattaag tatcaatatg aaaattcccg ttctccagcc tagtttcaac ttttttgccc
      601 ctgctggata ctctgctgcc gttgccccaa atcgtgcgga caatgcctat gcggattacg
      661 ttttggatat aggcaagcaa acaccacttt ccgcggcaga tttaagcaac gtatacgaaa
      721 atgtcattcg cgccgtccat gacagccgta gcaggctcat cgatcagcat acggtcgata
      781 tgattggcaa cactgtactt gatgctttga gtcgatcaca gacctttcgt gatgccgtaa
      841 gctatggcat tcataataag gaagtataca ttggtagcat taaatacaga aacgaatacg
      901 aacgtaacga agaatcccct gtcaaagttg atgatattca atcattaacc tgtaccgaat
      961 tatatgaata cgatgtcggg aaagaaccaa ttctccccat ttgcgaggca ggagaaaacg
     1021 agaacgaaga gccttatgtc agttttagtg ttgcgccaga tactgattct tatgagatgc
     1081 catcgtggca ggaaggactg attcacgaga ttattcatca tgttactggg gccagcgatc
     1141 catctggaga tagtaatata gagctaggac ccaccgagat tctcgcacgt cgtgtcgctc
     1201 aagagttggg atggagtgtt cccgacttca aaggatatgc agagccagaa cgagaagctc
     1261 atcttagact acgtaacctg aatgcccttc gacaggctgc catgaggcat gaaaagaatg
     1321 agagggcttt cttcgaaaga ctgggtacga tcagtgaccg atatgaggcg agtcctgatt
     1381 tcacagagta ttccgctgtg actaacatag gatacggatt tatccagcaa catgattttc
     1441 caggggtggc tatcgacgag aatttacagg atgtaaatca gatccaactg tatcatggtg
     1501 ctccttatat ctttacattt ggggatgtgg acagacacaa ttagcgctga ttcgtcttta
     1561 cagttacata gggttactac tcatagatat taacggagtt gatgatgggc aatcgtgcaa
     1621 cattgtattg tatgtaaagg cagagccccg aattttactg ttgtaagtgg gcctggcggg
     1681 aatggtatcg ccagcatcat tggcttgggt ccagtaaaaa gagacgcaga aagatgcaca
     1741 ataggcacta cgcgtcatgc atggattagg tcgctcatca tttacacagt actgctaaaa
     1801 aattattaag gaaagcgcgg cacttcgtat gtaagaacgt gtcgcggttg gctggtgaac
     1861 tttcgatagt gctagtattg gatgatttcc agccgttacc gattttatat aacttcagtg
     1921 gaattattca acacacaggc aaaacggaat aagctgagaa aaaaagcgtg ctttcccaat
     1981 ctggggtaaa ccacgtctga atgacacctg aaaacaggtg gtggcctcaa gaaagcttgg
     2041 aatattcttc tttaatgtta tgtaatttat tgatttttcg tgtacgattt taagacattt
     2101 attccaagaa aaatttttaa ctctttgatt tttcgactct gtatcatcgg tctcgaaaac
     2161 cggagtaggg gcaactctac cgggggttca aatccccctc tctccgccac tttatcaatg
     2221 acttatctcc cgacttcccg ccttgctttt cctaaacaga acaatcgtag aatattcttg
     2281 aagggttaga tcgtcactgt tttctgttcg atactgtgac attcagcact tgattcgcta
     2341 tggatctgac aggaagcttt cgagcgaaaa tgtgcagtta ttcagtcgtt ttcttatcgc
     2401 tcaccattat tcttttagac attcatccta tgaaactgcc gcaaagttgg tggtgggaac
     2461 tgaagttgcg tagagaaggg gtcaataatt gagtcaaaaa aggaacgcgg ccgtcagatt
     2521 aaagccgcgg gacaaagtcc ataatttcca agagttgttg aatttcccag cttttttgaa
     2581 agcctttgta tcatactaac aagtaaaaaa ggtttaaata ccaaaattaa cctgtatttt
     2641 tgataagtct taccattgta cgatttacac agtttctgct cctccttgtt gatagtggaa
     2701 agggtgcatt gtgcacttgc gtaaacattt atgacgcgtt gatgtctatg gttcacattt
     2761 ccttgtcagg ggatcgactt atttgcggcg aggcatcatt cgtaacagag tattatcttt
     2821 ctgaatgtag tgatgaaaga gtgctgcgcc agcgtgagct gcgattaaaa agtaacctat
     2881 atttgcccag gtttcgtgaa tattttttat cagtgcttta atttcgctgt ttggggtaac
     2941 aaagggagac acattgaaac caaggaaact ccacgatttt ccactgtaag ccattaaagc
     3001 aatccccagc agaggaagtg caaggaaggt tatataaagc atgatgtgca ttagcgaagc
     3061 cgctttcatt tgccaggcag gtggcggtgg tacaatagaa ggatcactat aacggtgttt
     3121 tataatcagg cgtgaaaaca ttaacaccca aacgaatata cccgcattgt aatgtgtttc
     3181 tcgcatcagc agataagtac tactaccttt aggaaaccag ccacggagtt ccatcgcggc
     3241 ataggttatt gcaacgaaaa gcagcgttat ccaatgtaaa taaatttgag tttttgagaa
     3301 tcggttcatt tttgtagctc ctgtaatttt tacagtcaag ctatcccacg aaccttaaaa
     3361 aatccttata cagatgtggt tttaagaatt tgttatttaa aaaattgata gtgcacattg
     3421 taaccagcca atataagcaa tggcgtgagc acaggtatct ttctctgttg gccgtattgt
     3481 gtatggaatc cgttattggt taactcaatg cgggttagct tattaatata cagacacgtc
     3541 atagtgatga gcgtgtctgt agctagcttg cgttcagtgg cgtttgtgga tggaccagtg
     3601 gcagttatga tgacttacta aagcggcatc gaggcgttat catgagaata ctcaatgaga
     3661 catcatttcc tcgaccactt cttcgctact caactgatat ggataatacg ttggccagtt
     3721 ttccatttca tttaataaag attgctgtga atcattaccc ataaaaatgt gaa
//
LOCUS       DAZGTR010000243         3747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGTR010000243 DAZGTR010000000
VERSION     DAZGTR010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.245"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..468
                     /locus_tag="ACHLCY_004911"
                     /pseudo
     CDS             1..468
                     /locus_tag="ACHLCY_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    <1..91
                     /locus_tag="ACHLCY_004911"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(548..943)
                     /locus_tag="ACHLCY_004912"
     CDS             complement(548..943)
                     /locus_tag="ACHLCY_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0371976.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIG
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            complement(943..1902)
                     /locus_tag="ACHLCY_004913"
     CDS             complement(943..1902)
                     /locus_tag="ACHLCY_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0371977.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQSQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLRTLINDVAEIKNITHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            2175..3077
                     /locus_tag="ACHLCY_004914"
     CDS             2175..3077
                     /locus_tag="ACHLCY_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0371978.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAALSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(3028..3258)
                     /locus_tag="ACHLCY_004915"
     CDS             complement(3028..3258)
                     /locus_tag="ACHLCY_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001815791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HFP0371979.1"
                     /translation="MSPTPAGSGKSARPVPGRTRDTDHRQRLCRAASAGAVKRGAWRH
                     PLPEKKRAVARPSPSYFIHRNAPPCRLSTQVR"
     gene            3174..3386
                     /locus_tag="ACHLCY_004916"
     CDS             3174..3386
                     /locus_tag="ACHLCY_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371980.1"
                     /translation="MNAACGQCPASGRARDGRFYRSRLASVTVYASLSPFSDERPSSR
                     FRGITLPSNRRRLRYSTVGLTRYRTR"
     gene            3462..>3747
                     /locus_tag="ACHLCY_004917"
     CDS             3462..>3747
                     /locus_tag="ACHLCY_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFP0371981.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DVNDDWLQPASNEMYRVLKKDALMVSFYGWHRIDRFMAAWIRAGFSVAGHL"
BASE COUNT          837 a          863 c          963 g         1084 t
ORIGIN      
        1 gagttcgacc gcctctggaa aaaataatgc cactgctgct gcgtgagcag tacggggtcg
       61 gaccggtatg cagtaactgc atattgcccc gtcaacgtat taccactgtc agcaacagcg
      121 gtatcatcct tataaacgta gtgcctgtgc acagcgcgat gactggctga agaaagagat
      181 actgcgcgta tacgatgaga atcatcaggt atacggtggc gtaaagtctg acgtcagttg
      241 ttacaggaag gtatcagggt ggccagatgt atagtggcgc gcctcatggc ggttatggga
      301 cttgccggtg ttctccgggg taaaaaggtc cgcactaccg tcagccggaa agccgttgcc
      361 gcaggcgaca gcgtaaaccg ccagttcgtg gcagaacgtc ctgaccagct gtgggtggcg
      421 gattttactt acgtcagcac atggcagggc ttcgtctgta tggcgttcga tttagggcac
      481 taaaaccgct gctggtccct cattataaaa gaagagcagg taatagactt aacctgctct
      541 tcttttatta tttttttaac atagaaagat tgcgtctggt ttgttcttcg aaatcatctt
      601 ttgaagtgat gctctctgat ttgttttctg aagatgcccc tatgatgttt tttaacacac
      661 ttatatcaat tgatgtagat gtgtatcctg ttgtttgctc tataagtttc accagatttt
      721 ctgcagtgat tttctcactg aaaaaaacac tgattagcgt taacagtcgg gggtcaacag
      781 aataaagagc tgctccgcaa ataagagatt gtctgtagaa gtctccccgc atttgtatcg
      841 ggagagagtc tattgtttgt tgtgtcaggt tatcctgcgg ataaagtgtc gggtgcaggt
      901 atgccacaac tttctttctt tttttcggat cactgctttc cattacgcat gcaccattgc
      961 tttcaccaat gcaaactgag gttcatccac cttgttcact ttcagattcg ggaaatattg
     1021 ctttatgtga gggtaaataa gctcggctcc tccgccagtg agatagattc ggttcattcc
     1081 tgagaatgtt gaaatttcct gcctgatgct ttctgccaga gatttcactt ccctgtctat
     1141 tacatgagta atgtttttta tttcagctac atcatttatt aatgtccgga gatattcatt
     1201 atcatgtctg ttttttatta actcatcagc aataaaataa ttacatggtg tggaggcttt
     1261 cgtcagcgca ttcatgacgg tatgagtgat gcgggatgtc ccgatttctg aatacccacg
     1321 gatttcagaa atgccttcaa atgcaccgag aattaaacca caatccagag tggttcctcc
     1381 aagatcgata atcaggctgc gttcaagttt gtttacttta tcttttttta gtgattcaaa
     1441 tgcagctggc aaagattcag gcattacatt aactttttta attttaaatg tttccccttt
     1501 attcagagat atctcacgga gaacatttgc cttttttctt tctattcttt cttcatttgg
     1561 ttgattgtca ttatcaaaga attcggttac aggtaatgtc actgtcagtt ctacatcctg
     1621 tgactgaagg ccggatgtca gcagtgcatg atgtatggca agcaaattgg tggtgctgta
     1681 ttgataactg acatgtgttg tacctatgac agcagtactg cccaaatcat aggtgtattt
     1741 ttttccatcc acgacgtaat tgtaaacttt gccggcatta aacagtcccg ggttccagcc
     1801 ttctttaaag ctgtttcctg acagactggt tcttacctcg ccatcttcaa gccaggccag
     1861 ttttacgtta gttgaaccat catcgcatga tacttttagc atttttttct tgcctcgttt
     1921 tgagtttgta tttatctcaa attgagcatc ctttatctag atgcatcttt ttgagttttt
     1981 attgttcctt attgtgtctc attttgggtt catgtcaagc gatgaggctc ttttagagtc
     2041 tctttgcatt cttgttgtga ctcaatttga gtctctattg atttctcatt ttgcgtttcg
     2101 aactgagacg gaaactactt aaccaggccg catgtgagag gagaattccc atgccaaact
     2161 ggtgttcaaa tcgtatgcat ttttctggtg aaccggcaca gattgctgag attaaacgac
     2221 tggccagcgg tgcagtcaca ccgctttatc gccgcgccac aaatgaaggt attcagctgt
     2281 ttctggccgg aagtgccgga cttctgcaga ccactgaaga tgtgcggttt gaaccatgcc
     2341 ccggactgac ggctgccggg cgtggtgttg tatcgccgga gaatatcgcg ttcacccgct
     2401 ggctgacaca cctgcagaac ggtgtactgc tggatgaaca aaactgcctg atgctgcatg
     2461 aactctggct gcagagcggt actggacagc gtcgctggga agggttaccg gatgatgtca
     2521 gggaaaccat cactgtgcat ttcaccgcaa aaagaggtga ctggtgtgac atctggggta
     2581 acgaggatgt atcggtgtgg tggaaccgtc tgtgtgacaa cgtagtgccg gaaaaaacca
     2641 tgccgtttga cctgctgacg gttctgccga cccgcctgga tgttgaagtg aatggcttta
     2701 acggtggtgt tctgaacggt gttccttctg catatcactg gtatacagaa cggtatggcg
     2761 tgaagtggcc ttgtgggtat gacctgaata tcagcagtca gggggaaaac ttcattcagg
     2821 tggatttcga cacgccgtgg tgtcagccgg aaagcgacgt tgttgcagca ttaagccgcc
     2881 gtttcggctg cacgctggag cactggtacg ccgaacaggg ctgcaatttc tgtggctggc
     2941 agcgatatga gcgtggagag cttgttgatg tgctgtgggg ggaactggaa tggtcttccc
     3001 cgacagatga cgatgagctg ccggaagtca ccggacctgc gtggatagtc gacaaggtgg
     3061 cgcattacgg tggatgaagt atgacggaga cgggcgggca actgcccgtt tcttttccgg
     3121 taaaggatgt cgccatgctc ctctttttac tgcgccggct gacgcggcgc ggcataaacg
     3181 ctgcctgtgg tcagtgtccc gcgtccggcc gggcacggga cgggcgcttt taccgctccc
     3241 ggctggcgtc ggtgacagtg tacgccagcc tgtcgccctt ttctgatgaa cgcccttcaa
     3301 gccgctttcg cggcataacc ttgccgtcaa acagacgacg gctgcggtat tccacggtcg
     3361 gcctgacccg ttaccggacg cggtgaacag cccacaggca gcggggaacg ggcaccacag
     3421 gggtgccctc ccggtgccct ctgtaagaga aggagttttg tatgtcccgt tttgtcctcg
     3481 gtaactgcat cgatgttatg gcccgtattc ctgataacgc cattgatttc atactgactg
     3541 acccgccgta cctcgtcggt ttccgtgacc gttccgggcg caccattgcc ggtgacgtta
     3601 atgatgactg gctgcaacct gccagcaatg agatgtaccg cgtactcaaa aaagacgcgc
     3661 tgatggtcag cttctacggc tggcaccgta ttgaccgctt tatggcggcc tggatacgcg
     3721 ccggattcag cgtggcaggg cacctcg
//
LOCUS       DAZGTR010000244         3746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGTR010000244 DAZGTR010000000
VERSION     DAZGTR010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.246"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(36..356)
                     /locus_tag="ACHLCY_004918"
     CDS             complement(36..356)
                     /locus_tag="ACHLCY_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HFP0371982.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSISKEKRSLLDSQKRTKENLQHVNKSVEKLSFAIKE
                     HYFD"
     gene            complement(416..1756)
                     /gene="escN"
                     /locus_tag="ACHLCY_004919"
     CDS             complement(416..1756)
                     /gene="escN"
                     /locus_tag="ACHLCY_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFP0371983.1"
                     /translation="MISEHDSVLERYPRIQKVLNSTVPALSLNSSTRYEGKITNIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMESNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYNN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQSFIQQSTKDISSYEKTIENLFKVVA"
     gene            complement(1740..>3746)
                     /gene="escV"
                     /locus_tag="ACHLCY_004920"
     CDS             complement(1740..>3746)
                     /gene="escV"
                     /locus_tag="ACHLCY_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP0371984.1"
                     /translation="FKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIAINISTAL
                     LLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIYSFGNFVV
                     GGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGDMRAGVID
                     PLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGMWQFDMPF
                     SEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIVQQVSVNS
                     RPFLISAALMLVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKDAMGADLS
                     NSQNISPGAEPLILNLSNNIYSSDIAQQIEVMRWNFFEESGIPLPKIIVNPVKNNDSA
                     IEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNEANQQLAHLS
                     GMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKELQRQLGLS
                     KIVDILQRLVEENISIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHILGRYSVR
                     GTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERSEQIIGFLKNIMNPTGNGVILT
                     ALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT         1136 a          874 c          684 g         1052 t
ORIGIN      
        1 aaacaggctc tatcaaattt ctttttagag aaactctagt caaagtaatg ttcctttatg
       61 gcaaaagata atttttctac tgatttatta acatgttgca gattctcttt tgttcgtttt
      121 tgtgaatcta gcaatgaacg tttctccttc gaaatggagg attttttctc atacaatcgc
      181 tgcttttgct catcaaatgc attgttttta atctgatatt tatacaacga cactttatcc
      241 aaattcacca gcgatgcaca atacgcctgc ttagaggcaa tcgactcttt aatggattgc
      301 tcgcagtcat cgagtttacc atccacctct tttatctgag agttaatctt agccattgat
      361 tctctcaatc ggttcgctct gcttttacga atagataaaa ttctgtccaa catactcagg
      421 caaccacttt gaataggttt tcaatcgttt tttcgtaact actgatatct ttggtgcttt
      481 gctgtataaa gctctgaatt aatttcctat tttttattgc cttatccgct tcaggatctt
      541 gccccatcgt gtactcacca atacgaataa gcagctcaac attgttataa gtcgcaataa
      601 gctttttgca ttctgccgcc gcacgcaaat gctccgatgt aacaacgtta tgcataacac
      661 gactggcact taaaccaata tcaattgcag ggaaatggtt ttcctctgca agttctcggg
      721 taagtacgat atgcccatca agaatagagc ggacttcatc gccaatagga tcattaacat
      781 tatcgctttc taacaacacc gtataaatcg cggttattga accttttggc gcaggccccg
      841 ctcgctctaa taatttaggt aacgaggaaa aaacactcgg agggaatccc ccccttacat
      901 caggttctcc cgatgcaagc ccaacatcac gcgcggctcg ggcataacga gttacagaat
      961 ccatcattaa taatacattt ttaccctgat cgcggaagta ctctgcaata gtcgtcgccg
     1021 taaatgcggc tttcatcctt tccagtgcgg ggcggtctga cgttgtgacg accagcacac
     1081 atttagaaag cgtggattga ggtaaaagcg cgaggaattc atttacttcg cgaccacgtt
     1141 cgccgataag agcaaggaca ataatatctg cggatgcgcc attacaaatc atccccaaaa
     1201 gcgtactttt accaacgcct gaaccggcaa aaataccgat acgctgccca ataccgcaag
     1261 ttagtaaccc atcaattgcc ctgacgccaa gtataaatgg ctgatcaata acctgcctta
     1321 ataaaggatc tggtggttca gcatacaagc tgcgttcaaa cggaagatag ggagcagcaa
     1381 tattactctc cattggtctg ccaatgccat caacaagccg cccaagtagt gcgtctccaa
     1441 cacgaatctt gaactcatcc ccctgataac ttaaccactg accacagtac atgccggaaa
     1501 catgctcaaa aggcaacagg aaaacctcat cttcatcaat agcgatcacc tcggctaaac
     1561 gttgactggg ttctatcttg taaaaggcgc caatacgcgc ttttggtaaa cgcgccttaa
     1621 taatcgtccc gccgatattg gtaattttac cttcatatct ggtagacgaa tttaatgata
     1681 atgccggcac agtgctattg agcactttct gaatacgtgg gtatctttcc aatacagaat
     1741 catgctctga aatcatttac cgttcctaat actttaagtt cgatattatt cccaacctcc
     1801 tgaaaagaga gcacgggtac tgacgggaac gaaccttcaa tcattttctt cacatagcgc
     1861 ctgatatcta aagcagtcag aatgacgccg ttccccgttg gattcatgat attttttaag
     1921 aagccaatta tctgctcact tcgctccggt gagatattca gatacgagcc tgatgacgtt
     1981 tgtctgatcg actctcgtag ctcattttca atatcagagc caataagcca aacgttcaga
     2041 agtgtaccgc gaacgctata gcgacctaaa atatgccgac gcagggcgat acgaacgtat
     2101 tcgcacaaga taaccacatc tttttctttt gttgaccaaa aaataagcgt ttcaaagata
     2161 gttctcagat ccctaattga gatattttcc tctacgagac gttggagaat gtcaacaatt
     2221 ttgctcaaac caagctggcg ctgcagctct ttcacaagct cgttatattt tctctccatg
     2281 atgtccatca aataacgcgt ttcttgtacg ccgatgaact ctttggcatt agacaataca
     2341 agtttcttta gcaaaaacgt tatcttatca tttgttgttg cataaacatc catacctgaa
     2401 aggtgagcta gttgttggtt agcctcattc gttttatata cgatagagtt agtcgaaagc
     2461 ttttcctgaa caaattcgaa tgagatctct gcatgcccag cctcaaaata gacagtatca
     2521 tctataagag tatctttgta tattgactct tgatagagca aaaattctat tgcgctatca
     2581 ttatttttaa ccggattgac aataatctta ggcaatggaa ttccactttc ctcaaagaaa
     2641 ttccaccgca tgacctcaat ttgctgtgca atgtctgaac tataaatatt attacttaag
     2701 tttaaaatca acggctcggc gcctggtgag atgttttggc tattagacaa atcagctccc
     2761 atagcatcct tattattgcc agttccaaat gttcttttct tctgtaattt ccaagctatc
     2821 cccaacaggc aaaccgccag aaataagaag accaatgtag gaaagcccgg aataatcgcc
     2881 attacgagca ttagtgcggc actgattaaa aaagggcgac tattaacaga aacctgttga
     2941 acaatatcac ctgcaaggtt ttcttctttt tcgctttcac cgggcacacg agtaacaacc
     3001 acgcccgcgg tgacagagat aataagtgca gggatctggg caaccaaagc atcaccgaca
     3061 gacaatacag aaaacaggct aagtgcctca ctaaatggca tatcaaattg ccacatacca
     3121 atgagcacgc caccaaacag gttaactaaa acaataatga taccggcaat agcgtcccct
     3181 tttacaaact tcatcgcccc gtccattgaa ccataaaact ggctttcctt ttgcactcgg
     3241 gaacgaagaa cttttgcctc aagcgggtca ataacgcctg cgcgcatatc accatcaata
     3301 ctcatttgtt ttcctggcat accatcaagg gaaaaacgag cacttacttc agcaacacgc
     3361 tcggcccctt ttgtaataac cataaattga acaatggtga taatagtaaa aataaccagg
     3421 ccaacaacaa tattaccgcc aacaacaaag ttaccgaagg agtaaataat gtcacccgca
     3481 tcgtgatgca gcagaataag tctggttgta ctaacactga gcgacaggcg catcaacgtc
     3541 gtaatcaaca ggattgtggg gaaagaagtt agttcaagag gattttttat atagattgag
     3601 agcattaata aaagtaaagc tgtcgaaata ttaatcgcga taatgatatc caccacaact
     3661 gttggtagtg gaataatcat catcattaca gccataaaga agaagagagc cagaataaga
     3721 tcgtgatatg actctgcttt tttaaa
//
LOCUS       DAZGTR010000245         3713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGTR010000245 DAZGTR010000000
VERSION     DAZGTR010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.247"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            268..1638
                     /gene="glmU"
                     /locus_tag="ACHLCY_004921"
     CDS             268..1638
                     /gene="glmU"
                     /locus_tag="ACHLCY_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP0371985.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            1800..3629
                     /gene="glmS"
                     /locus_tag="ACHLCY_004922"
     CDS             1800..3629
                     /gene="glmS"
                     /locus_tag="ACHLCY_004922"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP0371986.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT          916 a          909 c         1009 g          879 t
ORIGIN      
        1 agatcctgct gatataaaac acccctgttt tcccgtttat tcattgatcg aaataagagc
       61 aaaaacatcc acctgacgct taaatgaagg tactgcctta attttctgca gacaaaaggc
      121 gtgacgatgg tcgaaaatgg cactttcgtc agcggggata atccgttatt gaacaattta
      181 tcctctgtcc atttcacgat gaaaaaaatg tagttttttc aaggtgaagc ggtttaaatt
      241 cgttctcaaa ttacagtcag gacgcgtatg ttgaataatg ctatgagcgt agtgatcctt
      301 gccgcaggca aaggcacgcg catgtattcc gatcttccga aagtgctgca tacccttgcc
      361 gggaaagcga tggttcagca tgtcattgat gctgcgaatg aattaggtgc agcgcacgtt
      421 cacctggtgt acggtcacgg cggcgatctg cttaaacagg cgctgaaaga cgacaacctt
      481 aactgggtgc ttcaggcaga gcagctgggt acgggtcatg caatgcagca ggccgcacct
      541 ttctttgccg atgatgaaga cattttaatg ctctacggcg acgtgccgct gatctctgtc
      601 gaaacactcc agcgcctgcg tgatgctaaa ccgcagggtg gcattggtct gctgacggtg
      661 aaactggatg atccgaccgg ttatggacgt atcacccgtg aaaatggcaa agttaccggc
      721 attgttgagc acaaagatgc caccgacgag cagcgtcaga ttcaggagat caacaccggc
      781 attctgatcg ctaacggcgc agatatgaaa cgctggctgg cgaagctgac caacaataat
      841 gctcagggcg aatactacat caccgacatt attgcgctgg cgtatcagga agggcgtgaa
      901 atcgtcgccg ttcatccgca acgtttaagc gaagtagaag gtgtgaataa ccgcctgcaa
      961 ctctcccgtc tggagcgcgt ttaccagtcc gaacaggctg aaaaactgct gttagcaggc
     1021 gttatgctgc gcgatccggc gcgttttgat ttgcgcggta cgcttactca cgggcgcgat
     1081 gttgaaattg atactaacgt tatcatcgag ggcaacgtga ctctcggtca tcgcgtgaaa
     1141 atcggcaccg gttgcgtgat taaaaacagc gtgattggtg atgattgcga aatcagtccg
     1201 tataccgttg tggaagacgc gaatctggca gcggcctgta ctattggccc gtttgcccga
     1261 ctgcgtcctg gtgctgagtt gctggaaggt gcacacgtcg gtaactttgt tgagatgaaa
     1321 aaagcgcgtc tgggtaaagg ctcgaaagct ggtcatctga cttacctggg cgatgcggaa
     1381 attggcgata acgttaacat cggcgcggga accattacct gcaactacga tggtgcgaat
     1441 aaatttaaga ccattatcgg cgacgatgtg tttgtcggtt ccgacactca gctggtggcc
     1501 ccggtaacag taggcaaagg cgcgaccatt gctgcgggta caactgtgac gcgtaatgtc
     1561 ggcgaaaatg cattagctat cagccgtgtg ccgcagactc agaaagaagg ctggcgtcgt
     1621 ccggtaaaga aaaagtgatt ctggccgggt aatccggtca catgggatga ggagataaca
     1681 taatctccct cccacaagca gtaactataa aaataacccc actctctaca aggctcgggg
     1741 cgcccgaaaa aacgggcata caggttgacc gacaacgata taaatcggaa tcaaaaacta
     1801 tgtgtggaat tgttggcgcg atcgcgcaac gtgatgtagc agaaatcctt cttgaaggtt
     1861 tacgtcgtct ggaataccgc ggatatgact ctgccggtct ggccgttgtt gatgcggaag
     1921 gtcatatgac ccgcctgcgt cgcctcggta aagtccagat gctggctcag gcagcggaag
     1981 aacatcctct gcatggcggt acgggtattg ctcatactcg ctgggcgacc cacggtgaac
     2041 cttcagaagt gaatgcgcat ccgcatgttt ctgaacacat tgtggtggtg cataacggca
     2101 tcatcgaaaa ccatgaaccg ctgcgcgaag agttaaaagc gcgtggctat accttcgttt
     2161 ctgaaacaga caccgaagta attgcccatc tggtgaactg ggagctgaaa cagggcggta
     2221 ctctgcgtga ggctgttctg cgtgctatcc cgcaactgcg tggtgcgtac ggtacagtga
     2281 tcatggactc ccgtcacccg gataccctgc tggcggcacg ttctggtagt ccgctggtga
     2341 ttggtctggg tatgggcgaa aactttatcg cttctgacca gctggcgctg ctgccggtga
     2401 cccgtcgctt tatcttcctt gaagagggcg atattgcgga aattactcgc cgttcggtaa
     2461 acatcttcga taaaactggc gcggaagtaa aacgtcagga tatcgaatcc aatctgcaat
     2521 atgacgcggg cgataaaggt atttaccgtc actacatgca gaaagagatc tacgaacagc
     2581 cgaacgcgat caaaaacacc cttaccgggc gcatcagcca cggtcaggtt gatttaagcg
     2641 agctgggacc gaacgccgac gaactgctgt cgaaggttga gcatattcag atcctcgcct
     2701 gtggtacttc ttataactcc ggtatggttt cccgttactg gtttgaatcg ctggcaggta
     2761 ttccgtgcga cgtcgaaatc gcctctgaat tccgctatcg caaatctgcc gtgcgtcgta
     2821 acagcctgat gatcaccttg tcacagtctg gcgaaaccgc ggataccctg gcgggcctgc
     2881 gtctgtcgaa agagctgggt taccttggtt cgctggcaat ctgtaacgtt ccgggttctt
     2941 ctctggtgcg cgaatccgat ctggcgctga tgaccaacgc gggtacggaa atcggcgtgg
     3001 catccactaa agcattcacc actcagttaa ctgtgctgtt gatgctggtg gcgaagctgt
     3061 ctcgcctgaa aggtctggat gcctccattg aacatgacat tgtgcatggt ctgcaggcgc
     3121 tgccgagccg tattgagcag atgctgtctc aggacaaacg cattgaagct ctggcagaag
     3181 atttctctga caaacatcac gcgctgttcc tgggccgtgg cgatcagtac ccaatcgcgc
     3241 tggaaggcgc attgaagctg aaagagatct cttacattca cgctgaagcc tacgctgcag
     3301 gcgagctgaa acacggcccg ctggcgctga ttgacgcgga tatgccggtt atcgtcgttg
     3361 caccgaacaa cgaactgttg gaaaaactga aatccaacat tgaagaagtt cgcgcgcgtg
     3421 gcggtcagtt gtatgtcttc gccgatcagg atgcgggttt tgtaagtagc gataacatgc
     3481 acatcatcga gatgccgcat gtggaagagg tgattgcacc gatcttctac accgttccgc
     3541 tgcagctgct ggcttaccat gtcgcgctga tcaaaggcac cgacgttgac cagccgcgta
     3601 acctggcaaa atcggttacg gttgagtaat cactatcata gataaaaaag ggctgccgac
     3661 cggtggccct tttttataac tgacatcaat aaattcatct atctttctat ata
//
LOCUS       DAZGTR010000246         3706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGTR010000246 DAZGTR010000000
VERSION     DAZGTR010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.248"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1054)
                     /locus_tag="ACHLCY_004923"
     CDS             complement(<1..1054)
                     /locus_tag="ACHLCY_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0371987.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMV"
     gene            complement(1122..3230)
                     /locus_tag="ACHLCY_004924"
     CDS             complement(1122..3230)
                     /locus_tag="ACHLCY_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0371988.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRSSSVVTKDYTFKRPGWAGRFDQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
BASE COUNT          778 a         1108 c          972 g          848 t
ORIGIN      
        1 ccaccatccg gcccgggtgc tgcgcgtcat acgtgaaagc gcgcacctgc gtattgctgc
       61 ggtcatatac cgccagcagt tcaccggctt ccgtatatgt gtaccgcacc agtggcgcag
      121 cgggcaggct ctccgggtat gccgggtcgt gcatcagcca caccgccgaa aggcggatac
      181 ccctgtcggg gccgtattcg gtaccgggca gtgtgtcggg gaacgctgag gctgagagag
      241 ggcgggaact gtcagaagaa gatagcgaag aggtgcgggc ctcttccgca cgctgcgcct
      301 gcgtggtcag caccagacgg aactcccgcc cggcaccgtc cgtcacgccg gtgatttccc
      361 cggtcaggtc accggcggcc tcacgccggt acgtcagcgt ccgcccgaag cggtccgcca
      421 gccccgtcag ctcccggtac ggcggcagcg gcgctggcag cacgtcctca gcacccggca
      481 cccgctctga ccagccgagt atccaccacg gcccctgtgc gctgttggtc gccaggtaaa
      541 gatgcgggct taaccggata tccggcggca gcgcccccca cagccgcgcc agcgtatggc
      601 cgtccggctg tgctgcctta ccaccgcgca ccagccacat tgactcactg cggctgtaca
      661 ccgcctcccc cggcagcagc ggctcaaagt gaatgctccg cccgccgttg tcgttgagta
      721 tcagtccgtc atcacgtagc tgtaagcgga tatcagaagg cgctttccag ccggggccga
      781 aaacgcccac cggtgcaggc gtcttcgtcc ggtagctgct gtaggtgcgg gagagaatga
      841 acggcagcgg gccgggcagc gcaaggtccg tctcgccggg cagcaccttc gcccccagca
      901 gcggatttac cgggttgccc gaagtcatcc cgcccggaca caccgagcac gccacgccgg
      961 tgggcgcgcc aattcttaca cctgccgaac cctggacaat gggaccgcca tactgagtca
     1021 tatctccctg acgcgccgct ggttttccgc tcatgattca aattccttct tctgtttatt
     1081 tttaatgctg gtttgttact gaattccgaa caggtacggg atcagtactc tctcatatcc
     1141 ggcgtatcct gcttttcatc aggggtcttc ggtggggatt tcgaggctgc acagttccag
     1201 ttaatcaaca gtgagtcacc attaacctgc tccttaccct gcaaatgaat ttttgtgccg
     1261 ttgagaaaaa tttgtccgtc cttcgtcagc accagccttg ccttaccaca gcagagctcc
     1321 agatgctcac cggcggagta aatcgcggtc tgcccggtat catccttttt cgttttacca
     1381 acggtgaagg taacgttatt tccgactttg acgtcataac ccagcccgac cctcaacgat
     1441 ttatgcaaac cgatctgcga ggactgcatc agagccaccg aagtgttcat aatgccacct
     1501 accgtcgtct ggtacgccac gcccacggtc agcgcacgaa caagtccaat cgtttccacc
     1561 tgaacagaac ccacgttaat gatctggtta ctgcccaccg tctctatctg gttaacgccc
     1621 accgtctgta tctgattgtt ggtaaccgca atcatctgat tgttgccaat ggtctcagca
     1681 tggttgttta tcacatccgt ggtgcggtta ttcagcacct cggtgttcat gttcttctgc
     1741 gcgtggatgt agacctgttc tttccctgtc gcatcgtcaa acttcagttc attaaacccg
     1801 ctgcccttat aggttttcga gcggatcgtc atctgcgtct tcgtccccgg caggctgccg
     1861 ggtgtgcggt tttcatggtg gtaggtacgc cccataataa tcggctggtc cgggtcgccg
     1921 ttgaggaagt ccacaatcac ctcctgaccc acacgcggta tcgccaggtg gccaaaaccg
     1981 gtgcctgccc acgcctgtgc cacacggatc cagcacgaac tgtcttggtc tgccgggtta
     2041 taacggtccc agttgaactt cacccgcacg cgaccatgtt cgtcgcagaa gatttcctcg
     2101 cctgccggtc ctgtcacgac agcgctctgc gggccgtcga ccagcggttt cagcaacggc
     2161 tgtggtcgcc atgttctgtc tgccgggatc accgcaaaat ggttctccag cgccgtcccc
     2221 gctccctgcc gtcctggcac cgcctgtggc tgttcgccgt gcagttcact tgccaccacc
     2281 tgccattccc ggttcaggtt cgcctgcgga tgccccgtca gcacaattcg tcgtcccggc
     2341 catatctccg gcgagcgact cattccccgc gcggtttctg cattgtttcg ccagccgtcc
     2401 atctgccagc gggcaaagtt ctgcccgtgg gcccccttga aacgtccggg gtagtcatac
     2461 acttcatact gtgtgcgctg gtaatcctgg tgctggcctt cctgatcaaa acgtccggcc
     2521 cagccggggc gtttaaaggt gtagtctttg gtcaccacgg aagaagagcg gatttgtgcg
     2581 ctgtagcgga actggctgat gcagagggtg ctcacctcgg tacgggtgtt cgggttccat
     2641 gggatttcaa aagattcggg cagatggcgg actgtgtcgc acagcaccag gctctggtcg
     2701 gtacttttgt aagcatgctc ctcataaaag aagatgcctt cctccgccgc catccggcac
     2761 aggaaatcgt aatcagtctc accgtactgg acacaaaact cacgggaagg atgcggctcg
     2821 ctgaacagcg gactccattc ggtcaccccg ttttcctgca acatcgtgcc gaggatgctt
     2881 ttgatgtcct cgttctggaa gatacggaag ttctggcgca gaccggcacg ccacagcggc
     2941 gggtgcacct tcatgctgta cagcatctgg tttttgtcgt tctccccgag ttcaaaccag
     3001 gtcaccacgc ctttcacccg gcgctgtact tcatcgccct gccatatcgt caggtaggcc
     3061 attttgtcca gcacctgcgc aaattcaagg gagagaaact gctggctgac cagggagaga
     3121 tcgagggaaa agagcgaaga gagtgactgg ttcagatgaa aggaaaccac cgcaaaagca
     3181 tccggcggca ggccgtccac ttccagtgtg aaacgtaatc cggttgacat agcaacctcc
     3241 gtgaaaatga ataataatcc gcacatattg catgtacaac atgtcgggcg gatgagataa
     3301 gcgagtcgac atctggcgtt accggggaat atcagaaaaa tgtcatggtt atgtgatgat
     3361 ttagtctggg attttacttc agagaaacgg gtgtctgttt agtcaacaaa ataatcacaa
     3421 acgcaacgga tgggttctcg acgttattca cgacaggctc gtacattaca gcgagtaata
     3481 aaaaacctga tatgcaaagt caaaccaata ttcataatgt ctttttattc acaacaataa
     3541 ataattcaat aacaatcatt gaaatgaatg atggtttgta taaggaatat acgctaccgc
     3601 cccaccctga taacatgatc tgcacattgc catcttaacg tcgttaaaca ctagcttaat
     3661 agtggatatt tcaaattagg aatttattaa attcacctga tgaata
//
LOCUS       DAZGTR010000247         3588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGTR010000247 DAZGTR010000000
VERSION     DAZGTR010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.249"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            69..761
                     /gene="fimC"
                     /locus_tag="ACHLCY_004925"
     CDS             69..761
                     /gene="fimC"
                     /locus_tag="ACHLCY_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0371989.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRLIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            792..3401
                     /gene="sfmD"
                     /locus_tag="ACHLCY_004926"
     CDS             792..3401
                     /gene="sfmD"
                     /locus_tag="ACHLCY_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP0371990.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERISLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGTVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDEPIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGGGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGRKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            3443..>3588
                     /gene="fimH"
                     /locus_tag="ACHLCY_004927"
     CDS             3443..>3588
                     /gene="fimH"
                     /locus_tag="ACHLCY_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016155910.1"
                     /note="involved in the regulation of fimbriae length and
                     mediates adhesion of type 1 fimbriae; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HFP0371991.1"
                     /translation="MACLCLANISWATVCANSTGVAEDEHYDLSNIFNSTNNQPGQIV
                     VLPE"
BASE COUNT          960 a          787 c          856 g          985 t
ORIGIN      
        1 gaaaccaaag caatcatttc tttatatttc ttatttttgc cgtcaggaat acacaaggcg
       61 tattaactat gatgactaaa ataaagttat tgatgctcat tatattttat ttaatcattt
      121 cggccagcgc ccatgctgcc ggagggatcg cattaggtgc cacgcgtatt atttatcccg
      181 ctgatgctaa acagactgcg gtatggatta gaaatagcca taccaatgag cgctttctgg
      241 tcaattcgtg gattgaaaac agcagcggtg taaaagaaaa gtcattcatc attacaccgc
      301 cactgtttgt tagtgaaccc aaaagcgaaa ataccttgcg tattatttac accggtccac
      361 cgctggcagc agatcgtgag tctctgttct ggatgaatgt taagacgatc ccttcggtag
      421 ataaaaatgc attgaacggc aggaatgttt tgcaactggc gattttatcg cgcatgaaat
      481 tatttctccg tctaattcaa ttacaagaat tacccgcaga agcaccggac acactcaagt
      541 tttcgcgatc cggtaactat atcaatgttc ataatccctc acctttttat gtcactctgg
      601 ttaacttaca agtgggcagc caaaagttgg ggaatgctat ggctgcaccc agagttaatt
      661 cacaaattcc cttaccctca ggagtgcagg gaaagctgaa atttcagacc gttaatgatt
      721 atggttcagt aaccccggtc agagaagtga acttaaacta accgaatcat ctgacaatat
      781 cagagctaat tatgaaaata cccactacta cggatattcc gcagaggtat acctggtgtc
      841 tggccggaat ttgttattca tctcttgcca ttttaccctc ctttttaagc tatgcggaaa
      901 gttatttcaa cccggcattt ttattagaga atggcacatc cgttgctgat ttatcgcgct
      961 ttgagagagg taatcatcaa cctgcgggcg tgtatcgggt agatctctgg cgtaatgatg
     1021 agttcattgg ttcacaggat atcgtatttg aatcgacaac agaaaataca ggtgataaat
     1081 caggtgggtt aatgccctgt tttaaccagg tactccttga acgaattagc cttaatagca
     1141 gtgcatttcc cgagttagcc cagcagcaaa acaataaatg catcaattta ctgaaagctg
     1201 tacctgatgc cacaattaac tttgattttg cagcgatgcg cctgaacatc actattcctc
     1261 agatagcgtt gttgagtagc gctcacggtt acattccgcc tgaagagtgg gatgaaggta
     1321 ttcctgcttt actcctgaat tataatttca ccggtaacag aggtaatggt aacgatagct
     1381 atttttttag tgagctcagc gggattaata ttggcccgtg gcgtttacgc aacaatggtt
     1441 cctggaacta ttttcgcgga aatggatatc attcagaaca gtggaataat attggcacct
     1501 gggtacagcg cgccattatt ccgctgaaaa gtgaactggt aatgggagac ggcaatacag
     1561 gaagtgatat tttcgatggt gttggatttc gtggtatacg gctttactct tccgataata
     1621 tgtatcctga tagccagcaa gggtttgccc caacagtacg tgggattgcc cgtacggcgg
     1681 cccagctaac gattcggcaa aatggtttta ttatctatca aagctatgtt tcccccggcg
     1741 cttttgaaat tacagatttg cacccgacat cttcaaatgg cgatctggac gtcaccatcg
     1801 acgagcgcga tggtaatcag cagaattaca caattccgta ttcaacagtg ccaattttac
     1861 aacgcgaagg gcgtttcaaa tttgacctga cggcgggcga ttttcgtagc ggtaatagtc
     1921 agcaatcatc gcctttcttt tttcagggta cggcactcgg cggtttacca caggaattta
     1981 ctgcctacgg cgggacgcaa ttatctgcca attacaccgc ctttttatta gggctggggc
     2041 gcaacctcgg gaactggggc acagtgtcgc tggatgtgac ccatgcgcgc agtcagttag
     2101 ccgacgacag tcgtcatgag ggggattcca ttcgcttcct ctatgcgaaa tcgatgaaca
     2161 ccttcggcac caattttcag ttaatgggtt accgctattc gacacaaggt ttttataccc
     2221 ttgatgatgt tgcgtatcgt cgaatggagg ggtacgaata tgattacgat tacgacggtg
     2281 agcatcgcga tgaaccgata atcgtgaatt accacaattt acgctttagc cgtaaagacc
     2341 gtttgcagtt aaatatttca caatcactta atgactttgg ctcgctttat atctctggta
     2401 cccatcaaaa atactggaat acttcggatt cagatacctg gtatcaggtg gggtatacca
     2461 gcagctgggt tggcatcagt tattcactct cattttcgtg gaatgaatct gtagggatcc
     2521 ccgataacga acgtattgtc ggacttaatg tttcagtgcc tttcaatgtt ctgaccaaac
     2581 gtcgctacac ccgggaaaat gcgctcgacc gcgcttatgc ctcctttaac gccaaccgta
     2641 acagcaacgg gcaaaatagc tggctggcag gtgtaggtgg gaccttactg gaaggccaca
     2701 acctgagtta tcacgtaagc cagggtgata cctcgaataa tgggtatacg ggcagcgcca
     2761 cggcaaactg gcaggccgct tacggtacgc tggggggcgg gtataactac gaccgcgatc
     2821 aacatgacgt taactggcag ctgtctggcg gtgtggtcgg gcatgaaaat ggcataacgc
     2881 tgagccagcc tttaggggat accaatgttt tgattaaagc gcctggcgca ggcggtgtac
     2941 gcattgaaaa tcaaactggc attttaaccg actggcgcgg ctatgcggtg atgccgtatg
     3001 ccacggttta tcggtataac cgtatcgcgc ttgataccaa tacgatgggg aattccatcg
     3061 atgttgaaaa aaatattagc agcgttgtgc cgacgcaagg cgcgttggtt cgtgccaatt
     3121 ttgatacccg cataggcgtg cgggcgctca ttaccgttac ccagggcaga aaaccggtgc
     3181 cgtttggatc actggtacgg gaaaacagta ccggaataac cagtatggtg ggtgatgacg
     3241 ggcaagttta tttaagtggt gcgccattgt ctggtgaatt actggttcag tggggagacg
     3301 gcgcgaactc acgctgcatt gcgcactatg tattgccgaa gcaaagctta cagcaagccg
     3361 tcactgttat ttcggcagtt tgcacacatc ctggctcata aaggaaatta tcaataagat
     3421 aatctgcaga ttattattgg cgatggcatg tttgtgtctg gcaaacatat cctgggctac
     3481 tgtttgtgca aatagtactg gcgtagcaga agatgaacac tatgatctct caaatatctt
     3541 taatagcacc aataaccagc cagggcagat tgttgtttta ccggaaaa
//
LOCUS       DAZGTR010000248         3544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGTR010000248 DAZGTR010000000
VERSION     DAZGTR010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.250"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..978)
                     /locus_tag="ACHLCY_004928"
     CDS             complement(<1..978)
                     /locus_tag="ACHLCY_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0371992.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTVVVNTVASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEA
                     LRRFELMVEEAARHAEEAKKNAGEAETSARNAGISASQAEESAANADTSAGDASESAR
                     QAAESAAAAKQSEEASSSSASAAAQKASESLQSATDAELSKKTAESAAGNAARDATTA
                     AEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGD
                     TGPVGATGERGPRGDTGPAGPQGPKGDRGERGETGLTGNAGPQGPKGDTG"
     gene            complement(1130..1729)
                     /locus_tag="ACHLCY_004929"
     CDS             complement(1130..1729)
                     /locus_tag="ACHLCY_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0371993.1"
                     /translation="MRKLCAVILSAVVWQVAAATPASAAEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFMDALGLITSFSYANAEDEQKTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRSDAFIVGIGYRF"
     gene            complement(1797..>3544)
                     /locus_tag="ACHLCY_004930"
     CDS             complement(1797..>3544)
                     /locus_tag="ACHLCY_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0371994.1"
                     /translation="RENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAV
                     QHLTVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGKGTDARLVTTAITADTEHRF
                     SGLPLGEYTLTVRAINSYGQQGEPATTTFRIAAPAAPSRIELTPGYFQITATPHLAVY
                     DPTVQFEFWFSEKRIADIRQVETAARYLGSALYWIAASINIKPGHDYYFYIRSVNTVG
                     KSAFVEAVGRASDDAEGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITNV
                     SNEITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGI
                     ENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGG
                     NPPAFSLTPDGRLTAKNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKT
                     VGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKHEKEHTDIYSSCR
                     LIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDL
                     LVVVMKKATAGITIS"
BASE COUNT          720 a         1088 c          853 g          883 t
ORIGIN      
        1 accggtatct ccctttggac cctgtggacc tgcatttccc gtcagaccgg tctctccccg
       61 ctctcccctg tcacctttcg gcccctgcgg acctgccgga cctgtatctc ctctcggtcc
      121 ccgttcaccg gttgccccga cagggccggt gtctccacgc tctcctttat ctcccttcgg
      181 cccctgagga cccgcgggcc ccggttcccc ctttggcccg ggaggcccca ccacggtggg
      241 gattcggttt acggcttctt ccgccgctat cctgctttgt tccgctgact gtgcgctttc
      301 tgctgactcc cgggcttttt ctgctgcggt cgttgcatcc ctggctgcat taccggctgc
      361 actttctgcc gtcttttttg acaactcagc atctgttgca ctttgtaatg actcactggc
      421 tttttgagcg gccgcagagg ccgaggacga ggacgcctcc tctgactgct ttgcagcggc
      481 tgcactttct gccgcctgcc gggctgactc cgatgcatcc cctgctgaag tgtcagcatt
      541 tgcagcgctc tcttctgcct gactggctga tatgccggca ttcctcgctg acgtctccgc
      601 ctctccggca ttcttcttcg cctcctcagc gtgacgcgcc gcttcttcca ccatcagttc
      661 aaaacgacgc agtgcctccg gccggacgtc atcctccgac atggcaccga gaaaatcatt
      721 cagcgtcccc ggttgagaat cttcatacac ggtgatggtc ccggcatgtg acggaggaaa
      781 tccctccacc aacagaatga cgctgtactg accgtactca acatccatgc tgtaacgtcc
      841 ggcttcatcc ggattttcag aggccaccgt gttcaccacc accgtgctgc tggttcgtct
      901 ggccttcagc acaatggtgc agttctgtac tggttttcct gtgccatctt taagcacgcc
      961 agaaattttt actgtcatac ttttccacca ataaaaaaag cccgcagcag tgacgccacg
     1021 ggcttcagga cagtgtaact ttacgtttcc tcaaacgcag ttcaccccat aaggtggatg
     1081 aacctgcgta tcataacaat atttacagaa gataaatcgg cgtctgttgt cagaaacggt
     1141 atccgatacc aacaataaat gcatccgatc gccagtcgcc actaccggaa ccttcataag
     1201 caaggtcaat ggtcacggat tcggtcgggt taaactgcac gccagccccc cacgccagag
     1261 acgtgttgct gtggcgaccg tcatcacttc cggtcagcac atcgtgcgtt ttccccttgt
     1321 tgtcagttac gcggagataa tccccggaga aagtcgacac acggctgtaa gccacacccg
     1381 ccatcgcata cgcgctgaac cattcattca cgcgtacaga cggccccgcc atcacgctga
     1441 accagcggtt acgcacggaa tcttcatgcc agcgggtatc gctgtagcgc gttttttgct
     1501 catcctcagc attggcataa ctgaaggacg taatcagccc cagcgcgtcc ataaactcat
     1561 aacggtattt cacgttaatc ccgttcagat catcactacc gggaacgttc gtcgaggcat
     1621 ggagataccc cgcgctcagc gtggactgat gttctgctgc actcgctggc gtagcagcgg
     1681 cgacctgcca gactactgcg gacaaaataa cagcacataa tttacgcata attacctctc
     1741 gcttttctgc aataaaaaag gcgccatttc tggcgcccgt atctgggtta taaaattcag
     1801 ctaatcgtga tgcctgcagt ggctttcttc atcaccacaa ccagcaaatc gctgatactt
     1861 gctgtgggat accagttatt taccagccat gctgacaccg aaaactccag tgtcatgtga
     1921 ccgtgaccgg caggcatatc aataacacca ctgtaaatca gcgtattatc cagcgcggta
     1981 cggttataaa tttcagcacc gtttttccgc actatcagac ggcatgagga gtaaatatca
     2041 gtatgctctt tctcatgttt agcgccgctg aatgccaccg ccggaataac aatctgccgg
     2101 tcaaacggct gatcgtcata aaccctgacg gtaatggttc ctgatggcca ccgctccggt
     2161 gcacgggagt cccgggggaa agctttgccc actgttttaa cgagatcgcc ttcaatctgg
     2221 ttcgcggaca attttcccag aacccgacag ttctcgttaa tcgtgacgtt gttgagcgtc
     2281 ccggagttcg cattcacgtt accgctgata tcggcatttt tcgccgtcag ccgcccgtcc
     2341 ggtgtcaggg aaaatgccgg aggattaccg ccgctggtaa tggtgggagc cgtcagatat
     2401 ttcaggaaca cttcattcat aaatatctga tcgccctgac caacaaacat cggctttgtg
     2461 ttgccattcg caggattaat catcgcaatc ctgtctgccg ccagcagcac ctgactctgc
     2521 attcctgctg gcgtattctc aataccggca ccgatacccg caatataaag gcgtccgtcc
     2581 tgcatctgct gcagtttcac ggcccacatg ctgttcaggt tattatttgt atcaacctga
     2641 actttctgta tctgctggat tgccgcactc tgattttcca gttttttatt gacggtctgc
     2701 gtgatttcat tgctgacatt cgtaatggac gtcctgattt cagccaggtc cggcgcaagc
     2761 tgaccgttat caatctgcgt ccacagctcc tgggccagat gtgttttccc gatttctcct
     2821 ttgaaaaaat ccaggtaacc ttccgcatca tcgctcgccc gaccgacagc ctccacgaat
     2881 gccgatttgc caacagtatt cacactgcgg atataaaaat aataatcatg gcccggtttg
     2941 atattgatac tggcagctat ccagtacagc gccgagccaa gatagcgggc tgcggtttca
     3001 acctgcctga tatccgcaat ccgcttttcc gagaaccaga actcaaactg taccgtcggg
     3061 tcataaacgg caagatgcgg cgtggcggtt atctgaaaat agcccggcgt cagctcaatc
     3121 cgcgacggtg ctgccggtgc ggcaatccgg aaggtggtgg tggcaggttc accctgctgg
     3181 ccatagctgt ttatcgcccg caccgtcagg gtgtattccc cgagcggcag gccgctgaaa
     3241 cggtgctccg tgtctgcggt gatggcggtg gtcaccagtc tggcatccgt tcccttacca
     3301 ctggtcaggc gcagactgaa gcgcacgccc ttcaccaccc gcggcgtgtc ccatttagcc
     3361 tgcgccagat actggccgtc agctgcactc acctccaccg tcaggtgctg tactgccggt
     3421 gggatgacgc tgttcaggga acctgactgc ggctcaaagc gggcaccgtt atccacgatg
     3481 gcttcttttt ccggtacgtg ctgcaccgcc gtgatggcaa aggtgccgtc cgtgttttcc
     3541 cgga
//
LOCUS       DAZGTR010000249         3537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGTR010000249 DAZGTR010000000
VERSION     DAZGTR010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.251"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            58..984
                     /gene="allS"
                     /locus_tag="ACHLCY_004931"
     CDS             58..984
                     /gene="allS"
                     /locus_tag="ACHLCY_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP0371995.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            1053..2147
                     /gene="mnmH"
                     /locus_tag="ACHLCY_004932"
     CDS             1053..2147
                     /gene="mnmH"
                     /locus_tag="ACHLCY_004932"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP0371996.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            2263..2628
                     /gene="ylbG"
                     /locus_tag="ACHLCY_004933"
                     /pseudo
     CDS             2263..2628
                     /gene="ylbG"
                     /locus_tag="ACHLCY_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(3149..3322)
                     /locus_tag="ACHLCY_004934"
     CDS             complement(3149..3322)
                     /locus_tag="ACHLCY_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371997.1"
                     /translation="MAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFI
                     TNIGAGLPDACAE"
     gene            complement(3379..>3537)
                     /locus_tag="ACHLCY_004935"
     CDS             complement(3379..>3537)
                     /locus_tag="ACHLCY_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0371998.1"
                     /translation="DESLVSKHYINYMAIPENDGVFTWLPDFFPHVAVDISIYTNVED
                     DYFFLIFP"
BASE COUNT          906 a          845 c          878 g          908 t
ORIGIN      
        1 taaatcagag gataagaaaa tcacgcaact tgcggattta aaaggggata cctgaagatg
       61 ttcgatccag aaaccttgcg gactttcatt gcggttgctg aaacaggaag tttttcaaaa
      121 gcggcagaac gattatgtaa aaccacggcg acgatcagtt atcgcattaa acttctggaa
      181 gagaataccg gagtagcgct gtttttccgc acgactcgca gcgtgacgtt gacagcggct
      241 ggcgagcatc tactttccca ggccagagac tggctgagct ggctggaaag tatgccaagc
      301 gagctgcaac aggtgaatga tggcgtggaa cgccaggtga atattgtcat caacaacctg
      361 ctctacaacc cccaggccgt cgcccagttg ctggcgtggc tgaatgaacg ttaccccttt
      421 acccagtttc acatctcccg acaaatctat atgggcgtct gggattcgct attatacgaa
      481 ggtttttcac tggctatcgg cgtcacggga accgaggcgc tggcaaatac ctttagtctt
      541 gatcccttag gatcggtgca atggcgcttt gtcatggcgg cggatcatcc gctggcgaac
      601 gttgaagagc cgctaacaga agcgcagttg cggcgctttc cggcggtcaa tattgaagac
      661 agcgcccgca ccttaaccaa acgcgtcgcc tggcgattgc ctgggcaaaa agagattatt
      721 gttcctgata tggaaacgaa aatcgccgcc catcttgcgg gcgttggcat tggttttttg
      781 ccaaaatcac tttgccagtc aatgatcgat aatcaacaac tagttagccg ggtaatccca
      841 acgatgcgcc ctccttcgcc attgagtctg gcatggcgca aatttggcag cggcaaagcg
      901 gtagaagata ttgtgacctt gtttacccag cgcaggccgg aaatcagcgg atttttagaa
      961 attttcggca acccacgcag ttaaaaatca agtttccttg cgcgttggcg cgtaaaatag
     1021 cgcgcaagct gatttgaggt tcacttttaa ctatgcaaga gagacacacg gaacaggact
     1081 atcgtgccct gctgattgct gatacgccca ttattgatgt tcgcgcccct atcgagtttg
     1141 agcaaggcgc aatgcccgcc gctatcaatc tgccgttaat gaataacgat gaacgcgccg
     1201 ccgttggcac ctgctataaa cagcaaggct cagacgcagc gctggcgctg gggcataaac
     1261 tggtggcggg tgaaattcgt cagcagcgca tggacgcctg gcgggcagcg tgcctgcaaa
     1321 atccgcaagg tattctctgc tgcgcccgtg gcggtcagcg ctcacatatt gtgcaaagct
     1381 ggttgtatgc agcggggatt gattatccgc tggtggaagg cggttataag gcactgcgcc
     1441 agaccgcgat tcaggcgact attgaactgg cacaaaaacc gatagtgctg attggcggtt
     1501 gtactggcag cggtaaaacg ctgttagtgc agcaacagcc gaacggtgtt gatctggaag
     1561 ggctggcgcg ccatcgcggt tcggcgtttg gtcgcacgtt acaaccacaa cttagccagg
     1621 cgagttttga aaacctgctg gctgccgaaa tgctgaaaac cgacgcccgt caggatttgc
     1681 gcctgtgggt gctggaagac gaaagccgga tgatcggttc gaatcacctg ccggaatgcc
     1741 tgcgcgagcg aatgactcag gcggcgattg cggtggtaga agatccgttt gagatccgtc
     1801 ttgagcgcct gaacgaagag tatttcttgc gtatgcatca tgattttacc cacgcttatg
     1861 gcgacgaaca gggctggcag gaatattgcg aatacctgca tcacggactt tcggcgatta
     1921 agcgtcggct ggggttacag cgctataacg aactcgctgc ccagctggat acggcgctga
     1981 caacgcaact caccaccggc agcaccgacg gtcatctggc ctggctggtg ccgttactta
     2041 aagaatatta cgacccaatg tatcgctatc agcttgagaa aaaagcggaa aaagttgtct
     2101 ttcgcggtga gtgggcggaa gtggcggaat gggttaaggc gcggtaatga gggacgtatt
     2161 tgtattagtc gcgacttgat ctgacacatg gccttgaaag gttgagagtt accggttttg
     2221 atatgagtgt cgaatcctta tccaaaacaa gaggtaactc tcatgcttca tactgccaat
     2281 cccgtcatca aacacaaagc cggtttgctc aatctggctg aagaactcag caacgtatca
     2341 aaagcctgta aaatcatggg cgtttcgcgt gatacgtttt atcgttaccg cgaactggtc
     2401 gctgaaggcg gtgtggatgc gcagattaat cgtagtcgcc gcgctcctaa ccttaagaac
     2461 cgtaccgatg aggcaactga acaggctgtt gttgattacg ccgtcgcttt cccgacacat
     2521 ggtcagcacc gggccagcaa cgagctgcgt aaacaggggg tttttatctc cgacagtggt
     2581 gtccgttctg tctggttact ccataacctt gagaatctca aaagacgata ctgaacaaat
     2641 tttatcaggt gacgttccgt aaaaagttgt atggagttct cgatgcagtc ggcgcgacta
     2701 tcagtacccc cgctgaaccc tggactatcg ggccgccgta ctggggcatg tcaccctggc
     2761 gtgccgctgg ttttccgcta atggtttatt tcctctttct ttcccggcaa gaatttattt
     2821 taatccggaa ggattattta ttttcagata gggactcaca ttaaacacgt aacattttaa
     2881 ttaatgcatg gctgaatatg tattacacaa tgtcagatat cgtaaaagac cagtgcaatt
     2941 ctatgtaaac tctctgactg ttcattttat ttgttgtttc agggtcggaa ctcaccctca
     3001 ataaacaact gaatacgagt agatattcat cgtctgagct atatggcttt acacaatagc
     3061 cgacatgtct tttctcagtc aggaatttta cttttcctca ctacgaagtt gacgacttcg
     3121 atatgggata gactcttaat tcaagcaatt actctgcaca agcatcaggc aatccagctc
     3181 caatatttgt tataaaataa tgcgtaacat taatttcacc aacaaaaaaa aggtttacac
     3241 catccccttg ccctggtatc ttaccatatt tacaccacac tttttttgta tctggatcat
     3301 tctttgatac gattttcgcc acttgttccc tggccctcac tgtcaatgtt ttcttaaacc
     3361 tacccccatc atcatttgtt agggaaaaat aagaaaaaaa taatcatctt ctacatttgt
     3421 gtatattgat atatccaccg ctacgtgcgg aaaaaaatct gggagccatg taaaaactcc
     3481 atcattttct ggtattgcca tatagtttat gtaatgttta cttacaagag attcatc
//
LOCUS       DAZGTR010000250         3469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGTR010000250 DAZGTR010000000
VERSION     DAZGTR010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.252"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..215)
                     /locus_tag="ACHLCY_004936"
     CDS             complement(<1..215)
                     /locus_tag="ACHLCY_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0371999.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGEPQVGPSSEPDWYYVIVLAG"
     gene            192..433
                     /locus_tag="ACHLCY_004937"
                     /pseudo
     CDS             192..433
                     /locus_tag="ACHLCY_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(514..672)
                     /locus_tag="ACHLCY_004938"
     CDS             complement(514..672)
                     /locus_tag="ACHLCY_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0372000.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(758..1501)
                     /locus_tag="ACHLCY_004939"
     CDS             complement(758..1501)
                     /locus_tag="ACHLCY_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372001.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(1754..2377)
                     /locus_tag="ACHLCY_004940"
     CDS             complement(1754..2377)
                     /locus_tag="ACHLCY_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0372002.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGIVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(2374..3039)
                     /locus_tag="ACHLCY_004941"
     CDS             complement(2374..3039)
                     /locus_tag="ACHLCY_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFP0372003.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(3036..>3469)
                     /locus_tag="ACHLCY_004942"
     CDS             complement(3036..>3469)
                     /locus_tag="ACHLCY_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0372004.1"
                     /translation="QKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQE
                     AVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
BASE COUNT          982 a          857 c          687 g          943 t
ORIGIN      
        1 tgcccggcca gtacgatgac gtagtaccaa tccggctcag atgaagggcc gacctgtggc
       61 tctccttcaa tagccaccgc ctgcatcagt gtgtacggcg taatggcaac cggtccgccg
      121 tatggctgcc agccctcttt cagtttgtgt gtcagctttt ccgcaaggtc tgacggcgac
      181 gccgccctga caacatcgta atgtttaatc gacatcgaat ttctcccgtg tagaggaaca
      241 gagttaaaaa gccggaagcg gaatcaaatc acaggatgac catctgccag tggcaggtca
      301 taaaaaaaag gctgcgcaat gcgcagccag aactcacaag gaaaatgata aaaggaataa
      361 cactagtgat gtacgcatgg cgcctcccgc taagttctgc aatgatcaaa cagaactcgc
      421 tacgtgccct taaaactcga tcatttagcc cctccaagga ggattcacca tgcggttgat
      481 tttttaataa acagtaaaca aaaaagtcaa gaattattca ttctgttctt tcatcatcgg
      541 ccacagcaat accacaatgc cgcagaccag agcgccatca gtcagtacca acattatcct
      601 gctggtgaaa tccatcatca ccatcactaa aagcaggatc acaacagcaa gcagacacag
      661 tttataaaac aatgttcaga aaacgcattc agcatgccta aggttctatt cctacgaata
      721 gccaacttgc aacttaaaat attatttatg cagccaatta aattctggtc cttacaatat
      781 caacctgaag attcttatct tgtgctgatt gataaatgac aaacctttta ctacctgcat
      841 tgaaagaagt agacaaaacc agacaattat cataacgagc aagaacataa taccaaccat
      901 cattataatt aatcatttca tattctttct taaactgtgg tttgtaatat cctgtcaaaa
      961 atgaaaaaag ccaaaaatat gccacaaaag caatcatcac aatctcaaaa aaatgttttt
     1021 ttataaatgg cttatcatag aagcatgata ccgataaaaa tcgcccataa gatcttatcg
     1081 aaattgtaac cgccagcgca atcgctgctg acagtagcaa aagaggtacc tgaatcttct
     1141 gtctcaatat agaaaactca ataattgccg gcacaaacaa taattccaca gcaaaataaa
     1201 ggcgaaatac atttagctct tgcatagaat gttttctttt cactgcgaaa aagaatacaa
     1261 caccaatacc ccaaccgata agaaatatag caatgacgat aactgcaaaa aataaacttc
     1321 tggcaacatc atcaacacct gcacctacaa tccaccatgg gaagccgtag taaaaagaag
     1381 taccccatcc atagaaataa gcactccccc atccaaggca tcccatgtag gcaataaaaa
     1441 gtgaagaact cctgagcaac gcaccatcct tcataaccac cccaatacaa gatgataaca
     1501 ttggcttaca actcataaca aaagcaattc aatgccgtca agaggttaca ggctaaaaaa
     1561 actctattac attgcagtca gcatgtttac tacacaaata caattcagag cataaaaact
     1621 actcggcggc aggttattga gactcatcaa tgacatgtaa aaaacgccca ttattggtgt
     1681 caagtttccc caaagttatt caaaaagtca atattatgcc gttaatatgt tgccatccgt
     1741 ggcaatcatg gcgctaacgt gtgatcgcat tcaaaatgtt gtctgcgatt gactcttcct
     1801 tgtggcattg cacaaccaga gcgtcataca gcggcttaac agtgcgtgac caggtgggtt
     1861 gggtaaggtt tgggattagc atcgtcacag cgcgatatgc ggcgcttgct ggcattcttg
     1921 aataaccgac gcctttacat cttccgcact ctttctcagc aactatcccc cactgctctg
     1981 ttttggctat atcaaccgca cggcctgtac cgtggcaatc tctgcatctt gcgcccggcg
     2041 tagcggcact acggcaataa tccgcataag cgaatgttgc gagcacttgc agtacctttg
     2101 ccttagtatt tccttcgagc tttgccacac cacggtattt ccccgatacc ttgtgtgcaa
     2161 attgcatcag atagttgata gccttttgtt tgtcgttctg gctgagttca tgcttaccgc
     2221 agaatgcagc cattccgaat ccggcttgtg attgcgccat ccccatagca gccatcacat
     2281 cagtaccaga aagagagtca gaagccgtag cccgtggtga gtcgctcatc atcgggcttt
     2341 ttggcgaatg aaatttagct acgctttcga gtctcatgcg ccttctccct gtacctgaat
     2401 caatgtgagg tttccgcaga acactgcgcc agtatcgata tacatctggt tggcaaattt
     2461 gagtggtttc actgctggcg tatgaccaaa gatgaacgtg tccgcgcctt tgatttcttt
     2521 cacgatcccg tcttgtgagt tgctgattcg ttcgcggttc cagattacct gctgatgatc
     2581 aactggcttt ccaaactcgt atttatcaca aggataatcg gcgtggcaga tgacatattt
     2641 ttttccttta ctcaccagtt cgatgattaa cggaagttca tctgctttat gggcaagagc
     2701 tttagccaga atttctttgt cgtaatcgag attaaagaac cagccaccgc cattaagcag
     2761 ccagtgattg acgtttccac gctctgataa gccatcaatc atcatttgct catggtttcc
     2821 acgtacagct ctgaaccagg ggaatgtgat taattccagg cattctacgt tctctgtacc
     2881 gcgatcaacc aaatcgccca ccgagataag caggtctttt ttggtgtcga atcctatcgt
     2941 ctccagtttt ttcatcaggt tcgtgtagca tccgtgcaga tcgccaacta cccaaatatt
     3001 tcggtatttg ctgccatcaa ttctttcgta gatattcatg caacctcact tctgctgttt
     3061 cgcagttttt taagtttctg ttgatactcc gccttgatgg ccctgcactc ttcgacagtc
     3121 cagcgatagc ggttatggtt tgattcgatt tcctctactg cttcctgccc gatgcggcta
     3181 atcagttcga cgcgatacgg aacgagattt ccgcttttgt gctggttgca caccacgcat
     3241 tgcttgtgaa tattgcgttc atcaaatcgg agttgaggtg ccgcagcagt tgtccggtaa
     3301 tgtccggcat cccactgagc agacgtgagc gttccgcacg agatacatgg taagtcgcgg
     3361 tctctttctc tgatgaaggc gtttacggct tgttgggctt gtttaatcca gtaactgcgg
     3421 ggctttaagg cgagttttcg aatcttcagt ttatctttct gtttctgct
//
LOCUS       DAZGTR010000251         3464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGTR010000251 DAZGTR010000000
VERSION     DAZGTR010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.253"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            304..525
                     /locus_tag="ACHLCY_004943"
     CDS             304..525
                     /locus_tag="ACHLCY_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0372005.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGVL"
     gene            522..1568
                     /locus_tag="ACHLCY_004944"
     CDS             522..1568
                     /locus_tag="ACHLCY_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP0372006.1"
                     /translation="MKKPLVTRNEIAEAIALHTTCMPTREIPGAIANYFMITRRFYTR
                     TDKAVINRLLIAEIRDYLIEQGRLRYATVAAEMRKEARRMTGNNLNVEKTAPVASATP
                     SPAVNVISNTGDTIDSQTLLKMVNEARKLCGEPEVRNNKFIEKILDELEGEDGYTKSA
                     TVPPGGGTPMVVITMTYKQALRVAARESKAVRRSLIDKLEELQQANSPTPSIPQTLPE
                     ALRLAAELAEQKMQLEQQLVAAAPKVDFADRVSAANGILIGNFAKVVGLKQNALFSWL
                     RQNGILMAFGARKNVPRQQYINAGYFTVKEVVLDDEDGYQIRLTPQLTGKGQQWLTRK
                     LLDAGLLKPVASLS"
     gene            2208..3008
                     /locus_tag="ACHLCY_004945"
     CDS             2208..3008
                     /locus_tag="ACHLCY_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP0372007.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            3038..>3464
                     /locus_tag="ACHLCY_004946"
     CDS             3038..>3464
                     /locus_tag="ACHLCY_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0372008.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRANL
                     YRFTPSFLAFAQQAKNALIESKLKISSAATKVKAVLAKTL"
BASE COUNT          946 a          868 c          816 g          834 t
ORIGIN      
        1 tcgcgaatgc tatcttttcg catcatattg accttttaat cgttcaggct tatagttccg
       61 ccgtcgtagc aaattctgcg accaggtttg acagcctgaa tgattgtgcg gacaaccgca
      121 gatttccgat attgcggtat ttttgtgtcc gttaaaccgc gttacgccca aattatggtg
      181 gggcgtgatg gggaggcttc ggcctgctgg tttcacaatc gccagtctgt caaccctgtc
      241 acgtcctgcc acctgtttga cagcgggtag caggttgtta aacctgattg tgaggccgta
      301 actatggtta atgccaatcc ttgcgcacgc caagaattca tctggcgttt ctattcctgt
      361 aaaaaacacc actatcactt cgttatcgca gcaacagaag acgaagcacg ctctcaattg
      421 cctgatggcc cctgcatttt cactgcccgt ttttcaacta actcgcgcaa ttcacttagt
      481 tactggaacc tccccttctc tgccgacgtt cagggggttt tatgaaaaaa cctctcgtca
      541 cccgtaatga aatagccgaa gcgatcgctt tgcatacaac ctgtatgccg acacgggaga
      601 tccccggcgc aattgccaac tatttcatga taaccagacg tttttatacc cgaacagata
      661 aggctgtgat caacaggcta ctgatagccg agatcaggga ttatttgatt gaacaaggac
      721 gtctacgtta cgcaacagtg gcagcagaaa tgagaaagga ggcgcgcaga atgaccggta
      781 ataatttgaa tgttgaaaaa acagcacctg ttgcttcagc tacgccatca ccagctgtga
      841 atgttatctc caacactgga gacacaatcg acagccagac actgttaaag atggtcaatg
      901 aagcgcgtaa gttatgtggg gaaccagagg ttcggaacaa caaattcatc gaaaaaatac
      961 tcgacgaatt agaaggtgag gacggttaca caaaaagtgc aaccgtgccg ccaggtggcg
     1021 gtacgcctat ggttgtcata accatgacct acaaacaagc cctgcgagtc gccgcacgcg
     1081 aatcaaaagc cgtccgccgt tcgctgatcg acaaactgga agaattgcag caggcaaact
     1141 cccctacccc atcgatcccc caaacattac cagaagctct acgcctggct gccgagttgg
     1201 cagaacagaa aatgcagttg gaacaacagc tggtggccgc agcccctaaa gtcgattttg
     1261 ccgaccgggt atcagcggct aatggaatcc tgatcgggaa ctttgcaaag gtcgttggac
     1321 ttaagcaaaa cgcccttttc tcatggttgc gccagaacgg cattctcatg gcttttggtg
     1381 cgcgcaaaaa cgtaccgcgc caacagtaca tcaacgccgg atatttcacg gtgaaagaag
     1441 tggtgctgga tgatgaagat ggctaccaga tacggctgac gccccaatta acgggtaaag
     1501 gccagcagtg gttaactcgc aagctacttg atgctggttt gttaaaacca gtggcgtcct
     1561 tgtcttaacg tattcagacc aggatttgcc ataaattaca ggcatgtgta cgatatgccc
     1621 aaccctgttt ttcaggggag gcgcaacgtt atgcgggggt tatgaactcc tggatgcgta
     1681 gcctccagac agaaaaaacc ctcgctacaa cgttgcatgg aggcaaccag cgagggcatt
     1741 ccttatgtct gaacaacaag aggatagccg caagcagttg ctgttgcaag tatgcggagc
     1801 ttcgtaatga ctcttcaaaa agcttgcaag tggattgtga ccaacatcat tgttcctgtc
     1861 atcatcggag cttcaagttc tgtgttggca tcagtgattg cctcacttct ccacattgca
     1921 taacaacatt gaagggcgtt agttcgcagt gtaacagcag cgcgttgcgc ccgaacaggc
     1981 cacagggtaa aacctgtggc ctttttcgtt ttatccagcc acaaatccac attgaaaaac
     2041 gccttataca aaaaaacctg ccagcaaact ggcaggtttc tgagcagatc gtccaacccg
     2101 atctggatcg agtcagaaaa atttgctcta ataaatttcg ttttctaagt acaaagaatc
     2161 accatttcga gctggtgatt gaaggttgat gcaaatttgg agaaaaaatg caacaaacat
     2221 tcaatgcgga tatgaatata tcaaaccttc atcaaaatgt cgatccttca accactctgc
     2281 ccgttatttg tggtgttgaa attacgaccg accgcgctgg ccgttacaac cttaatgctc
     2341 tacacagagc gagcggactc ggtgcccata aagcgccagc tcaatggcta agaacgctgt
     2401 cagccaaaca gctcatcgaa gagcttgaaa aagaaactat gcagaattgc atagtttcgt
     2461 tcgaaggccg tggcggcggc acttttgccc atgaattgct cgcagtggag tacgcaggtt
     2521 ggatttctcc cgcgtttcgg ctgaaggtaa accagacatt tatcgactat cgagctggaa
     2581 gattacaacc tgctattccg cagagcctcc cagaagctct ccgtttggct gctgacctgg
     2641 cagagcaaaa gcaacggctg gagcaaaaaa tgctgatgga tgcacctaaa gtcgaattcg
     2701 ccgaacgcgt tgctaccgcc agcggggttc taatcggcaa ctatgccaaa gtgctcggcc
     2761 tgggccaaaa ctatctcttc acctggttgc gtgataacgg aattctgatc gcaaccggtg
     2821 aacgcaggaa cgtccccaaa caagaataca tatcccgtgg gtatttcacc cttaaagaaa
     2881 ccgtgatcga tacaagcaat ggaagcagga tttctttcac gactcgtata accggcaaag
     2941 gtcagcagtg gctgatgaag cgattgcttg atgctggtgt gctggtacct gtcgcggcaa
     3001 cgcgctaaca gacgtagtaa gaaccaccag cattgtaatg ctggctaaag tcactttcct
     3061 gagctgtata acgatgagcg attttacttt ttctggctat gaattggcct gctttgtaac
     3121 acactccggt ctatcccgta gcgccgggca tatcctgtcg caatgtgcaa atctcgcggc
     3181 aacaaccagt gaatacttca ttcacaagcc tcaccgcctg atcgcggcag aaactggtta
     3241 tagccaatca accgtcgttc gtgcattccg tgaagctgta aacaaaggaa tcctgtctgt
     3301 agagattgtt atcggcgatc accgtgaacg tcgcgctaac ctgtaccggt ttacaccatc
     3361 ctttttggcc ttcgcacaac aagccaaaaa tgcgctgatt gaaagcaaat taaagatctc
     3421 ttcagcggca accaaggtta aagctgttct cgctaaaaca ttgt
//
LOCUS       DAZGTR010000252         3456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGTR010000252 DAZGTR010000000
VERSION     DAZGTR010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.254"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            62..640
                     /gene="espA"
                     /locus_tag="ACHLCY_004947"
     CDS             62..640
                     /gene="espA"
                     /locus_tag="ACHLCY_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFP0372009.1"
                     /translation="MDTLNTASVASTNASTSTSLTYDLGSMSKDQVVELFNKLGVFQA
                     ALLMFSYMYQAQSDLSIAKFADMNEASKASTTAQKMANLVDAKIAEVQSSSDKNAKAQ
                     LPKEVLDYINDPRNDVTISGITDLNAALGAGDLQTVKAAISAQANNLTTTVNNSQLEI
                     QQMSNTLNLLTSARSDMQSLQFRTISSISLGK"
     gene            653..1795
                     /gene="sctE"
                     /locus_tag="ACHLCY_004948"
     CDS             653..1795
                     /gene="sctE"
                     /locus_tag="ACHLCY_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFP0372010.1"
                     /translation="MLNVNSDIQSVMSGVSAATTTSGINQPGGSSALDLQLVKSAAPS
                     ASWTESTALPTPPAGHSLVTPSVAEDVLSKLFGGISGDVMSRTSGTEPQSSTQSTTSG
                     YPYLSQVNKVDPQTMMMMATLLSLDASAQKVASMKNSNEIYKDGQNQALENKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAVAAIFNPALWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQAFGGLSLAAGILTAGIGGVSSLMSKVGDVAGKVGSNIVKAVTT
                     LADTLVENVASKISAVANGLTTSSRSIGTTVLNNDAANHNLLSQISSFVVENLTRQSE
                     SLSQSAKAELDKAALEVQNQANYIQSASKLISDSARVNSRIVSGRV"
     gene            1816..2778
                     /gene="espB"
                     /locus_tag="ACHLCY_004949"
     CDS             1816..2778
                     /gene="espB"
                     /locus_tag="ACHLCY_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFP0372011.1"
                     /translation="MNTIDNNNAAIAVNSVLSSTTGSASSTTTSTSSISSSLLTDGRV
                     DISRLLLEIQKLLREMVTTLQDYLQKQLAQSYDIQLAVFESQNKAISEKKAGATAALI
                     GGAISSVLGILGSFAAINSATKGASDAVQQAASTSAKSISTASEASTKALAKASESIA
                     DAADDAASVQQTIAAAAKAASRTSGVTDDVAASAQKASQVAEEAAGAAQELAEKAGLL
                     SRFTAAAGRISGSTSFIVVTSLAEGTKTLPTTISESVKSNHDISEQRAKSVENLQASN
                     LENYKQDVRRTQDDISSRLRDMTTTARDLTDLINRLGQSARLAG"
     gene            2785..3192
                     /gene="cesD2"
                     /locus_tag="ACHLCY_004950"
     CDS             2785..3192
                     /gene="cesD2"
                     /locus_tag="ACHLCY_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFP0372012.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFIMNVNS"
     gene            3227..3448
                     /gene="escF"
                     /locus_tag="ACHLCY_004951"
     CDS             3227..3448
                     /gene="escF"
                     /locus_tag="ACHLCY_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFP0372013.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
BASE COUNT         1001 a          680 c          812 g          963 t
ORIGIN      
        1 tgatttaatt gttttttaat agtttttgtc atgctaaaaa agattattaa gaggtatata
       61 catggataca ttaaatacag catcagttgc tagtacgaat gcgagtactt cgacatcgct
      121 gacctatgat ttagggagca tgtcgaaaga tcaggttgtt gaactattca ataaactcgg
      181 tgtttttcag gctgcgcttc tcatgttttc ctatatgtat caggcacaaa gcgatctgtc
      241 gattgcaaag tttgctgata tgaatgaggc atctaaggcg tcaaccactg cccaaaaaat
      301 ggcgaatctt gtggatgcta aaattgctga ggttcagagt agctccgaca agaatgcgaa
      361 ggctcaactt cctaaagaag tgcttgacta tataaatgat cctcgcaatg acgttacaat
      421 aagtggtatt accgatctaa atgctgcatt aggcgctggt gatttgcaaa cggtgaaagc
      481 agctatttca gctcaagcga ataatttgac aacgacggtg aataatagcc agcttgaaat
      541 acagcaaatg tcaaatacgt taaacctatt aacgagtgca cgttctgata tgcagtcact
      601 gcaattcaga actatatcat caatatccct tggtaaataa ccggagataa ctatgcttaa
      661 tgtaaatagc gatatccagt ctgtgatgtc tggggtcagt gccgctacga ctacttcagg
      721 tattaatcaa cctggagggt cttccgcgct ggatttacag ctggttaaat ccgctgcccc
      781 atcagcaagt tggacagaaa gtaccgcttt accaacgccg ccggcaggcc actcattagt
      841 gacgccctct gttgctgaag atgttttgag caaacttttt ggcggcatta gtggtgatgt
      901 tatgagccgc actagtggga cagaaccaca gagtagtaca caaagtacaa cctctggcta
      961 tccttatctt tctcaggtga ataaggttga ccctcagaca atgatgatga tggccactct
     1021 gttatccctg gatgcctcag cgcagaaagt tgcgagtatg aagaactcca acgagatcta
     1081 caaggatgga caaaatcaag cgctggaaaa taaaactctg gaatttaaaa aacagcttga
     1141 agaacaacag aaagccgaag agaaagcaca aaaaagtaaa attgttggtc aggtctttgg
     1201 ttggttaggg gttgcggtaa ctgctgttgc agctattttt aacccagctc tctgggctgt
     1261 tgttgctgtt agtgcaacag caatggcgct gcaaactgcg gttgatgtta tgggcgataa
     1321 ggctccgcag gcgttaaaaa cagcagcgca agcttttggc ggtctatctc ttgccgcagg
     1381 tattctgaca gccggcattg gcggggtgtc ttcacttatg tctaaagttg gcgatgtggc
     1441 tggcaaagtg ggttcaaaca tcgtaaaagc ggtcactacg ttggccgaca ctcttgtcga
     1501 aaacgttgca tcgaaaattt cggctgtggc aaatggttta accacttctt cgcgctctat
     1561 tggtacaact gtgctgaata atgacgcagc gaatcataat ctgttatcac agatttcttc
     1621 ttttgttgtg gaaaatttaa ctcgacaaag tgaaagctta agtcagagtg cgaaggcaga
     1681 gctggataaa gccgcactcg aggtgcaaaa ccaggcgaat tatatacaga gtgcttctaa
     1741 gctaatttct gattctgcgc gagtgaatag ccgtattgtc agcggtcgag tttaattgtt
     1801 aaagaggcgt ttataatgaa tactatcgat aataacaatg cggcaatcgc agttaattct
     1861 gttttgagca gcacgactgg cagcgccagt tcaacaacga catcaacctc gtcaatcagt
     1921 tcatctttac ttactgatgg cagagtggat atttctaggc ttctgctgga gattcaaaaa
     1981 ctgttgcgtg agatggtcac cacattgcaa gattatcttc agaagcaatt ggcgcaaagc
     2041 tatgacatcc aactagccgt ttttgagagc caaaataaag ctattagtga gaaaaaagcc
     2101 ggtgcgacag ctgctctgat tggtggtgct atctcatctg tcctggggat tttaggctct
     2161 tttgctgcca ttaatagcgc aacgaaagga gcgagtgatg ccgttcagca agccgcttct
     2221 acttctgcga agtctatcag tacagcctct gaagcttcaa ctaaagcact ggcgaaggct
     2281 tccgaaagta ttgcagatgc agcagatgat gcagcctcag tgcagcaaac tatcgcggca
     2341 gctgcaaaag cggccagtcg tacatctggt gtcactgatg atgtggctgc ttctgctcag
     2401 aaagcttctc aagtagctga agaggctgct ggtgctgctc aagaattagc agagaaggca
     2461 ggattattaa gtcgctttac tgctgctgct ggaaggattt ccggttctac gtcatttatt
     2521 gttgttacca gccttgctga aggtacgaaa acattgccaa caacgatatc tgaatctgtc
     2581 aagtccaatc atgatatcag tgagcagcgt gctaaatctg tagaaaatct ccaggcgtcg
     2641 aatttagaga actacaaaca ggatgttcgc agaacgcagg atgatatctc tagccgtctg
     2701 cgtgatatga caacgacagc ccgtgacctt accgacctta ttaatcgcct ggggcaatcg
     2761 gctcgcttag ctgggtaatt gatcatggtc gatacgttta atgatgaagt gcttaatcac
     2821 tatcttgaac aaaaagggta cacaatacag aaggagtttc tttgtggcag tgcctttttt
     2881 atcggatggc ggattgagac accttttttc tcattagcgt acagactgga tgaacaagaa
     2941 ctgattttgt gctcttttga agcacgtaac caaacagggc ttaacggccc tgttttatca
     3001 ctgactcgtt tgctcgaaga gttgtatcac catttttcgg gtattaagaa aatcagtgcg
     3061 atgaaatcta agattggttc agattcagaa cgtcaaaagc gcgaagagtt gtttaattat
     3121 ttcatcagaa agggtgccgt gcagcaagaa acagaagacg gaatttggtt cataatgaat
     3181 gtaaatagtt agggattttt attttattaa ctattaagga gatttaatga atttatctga
     3241 aattactcaa caaatgggtg aggtaggtaa aacactgagc gattctgtgc cagagttact
     3301 taatagcacc gatttggtta atgaccctga aaaaatgtta gagttgcagt ttgcggttca
     3361 gcaatattct gcttatgtta acgtagaaag tggaatgctg aaaacgataa aagatctggt
     3421 ctcaaccatt tccaaccgta gcttttaatt tttatg
//
LOCUS       DAZGTR010000253         3402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGTR010000253 DAZGTR010000000
VERSION     DAZGTR010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.255"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..115)
                     /locus_tag="ACHLCY_004952"
     CDS             complement(<1..115)
                     /locus_tag="ACHLCY_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015953002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HFP0372014.1"
                     /translation="MTSREARLGMFLASLLRRIAFSYYDYKAYNFNIEKTDF"
     gene            403..1691
                     /gene="shiA"
                     /locus_tag="ACHLCY_004953"
                     /pseudo
     CDS             403..1691
                     /gene="shiA"
                     /locus_tag="ACHLCY_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
     gene            1793..3247
                     /gene="amn"
                     /locus_tag="ACHLCY_004954"
     CDS             1793..3247
                     /gene="amn"
                     /locus_tag="ACHLCY_004954"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP0372015.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          792 a          828 c          811 g          971 t
ORIGIN      
        1 caaagtctgt tttttcaata ttgaaattat aagctttata atcgtagtaa ctaaatgcaa
       61 tacgtctcaa caatgatgct aaaaacatac ctaacctcgc ctccctactg gttataatac
      121 aatgcagtct atcagactca tcagggtgcc attttgtgca tatgcggact tttatgtttc
      181 atatctctaa cctgtgggtc ctctgcttaa tccttaaaca acaccagcaa ctcctgcgct
      241 ttcatcttcc atcgaatttt tcatgttgcc gctaatcagc cataaaaaca tttgcagatg
      301 cgctctgtcg aggtagtctc ataaggttcg tttatagatc gacggcaatg tgagttacct
      361 tttccatact aattataaaa agacagtaca aacaggatca ttatggactc cacgctcatc
      421 tccactcgtc ccgatgaagg gacgctttcg ttaagtcgcg cccgacgagc tgcgttaggc
      481 agcttcgctg gtgccgtcgt cgactggtat gattttttac tctatggcat caccgccgca
      541 ctggtgttta atcgcgagtt tttcccgcaa gtaagcccgg cgatgggaac gctcgccgca
      601 tttgccacct ttggcgtcgg attccttttc cgtccgctcg gcggtgtcat tttcggtcac
      661 tttggcgacc gactgggacg taagcgcatg ttaatgctga ccgtctggat gatgggcatc
      721 gcgacagcct tgattggtat tcttccttca ttctcgacca ttgggtggtg ggcacctatt
      781 ttgctggtga cactgcgtgc cattcaggga tttgccgtcg gcggcgaatg gggaggcgcg
      841 gcgttgcttt ccgttgaaag tgcaccgaaa aataaaaaag ccttttacag tagcggtgta
      901 caagttggct acggtgtagg tttactgctt tcaaccggac tggtttcatt gatcagtatg
      961 atgacgactg acgaacagtt tttaagctgg ggctggcgca ttcctttcct gtttagcatc
     1021 gtactggtac tgggagcatt gtgggtgcgc aatggcatgg aggagtccgc ggaatttgaa
     1081 caacagcaac attatcaagc tgccgcgaaa aaacgcatcc cggtgctttc ctgaagatta
     1141 ttgcgctacg actgtgcgaa ttgctgacga tgtacatcgt tactgccttt gcacttaatt
     1201 attcaaccca gaatatgggg ttaccgcgcg aacttttcct caatattggt ttgctggtag
     1261 gtggattaag ctgcttgaca attccctgtt ttgcctggct tgccgatcgt tttggtcgcc
     1321 gcagggttta tatcacaggt gcgttaatcg gaacgttgag cgcatttcct ttctttatgg
     1381 cgcttgaagc acaatctatt ttctggatag ttttcttctc cataatgctg gcaaacattg
     1441 cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac cgaaatgttt ggtgccagtt
     1501 atcgctatag tggtgctgga gtcggttatc aggtcgccag tgtggttggc ggtggattta
     1561 caccttttat tgccgctgca ctcatcactt actttgccgg gaactggcat agcgtcgcca
     1621 tttatttgct ggctggatgt ctgatttccg caatgaccgc tttgttgatg aaagacaatc
     1681 aacgcgcttg atagtctggc gaagatcatc cgatcttcgc cttacacttt tgtttcacat
     1741 tcctgtgaca tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa
     1801 taagggctcc ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca
     1861 atctgtagtc gcattacgca acgccattgg caactatatt acaagtggcg aattacctga
     1921 tgaaaacgcc cgcaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg
     1981 tagcacaacc aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta
     2041 caccaccacg attactcgcc ctactctctt tcgttcgtat ctcaatgaac aacttacgtt
     2101 gctgtatcag gattatggtg cgcatatctc ggtgcaaccc tcgcagcatg aaatccccta
     2161 tccttatgtc atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgggttaac
     2221 tcgctacttc ccgacaacag aactggcgca aattggcgat gaaactgcag acggcattta
     2281 tcatccaact gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct
     2341 cgcgcggttg cgccattata ccggtacgcc agttgaacat tttcagccgt tcgtcttgtt
     2401 taccaactac acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga
     2461 tcctgatagt ccctacattg ccctttcttg tgctggcggg aactggatca ccgccgaaac
     2521 cgaagcgcca gaagaagcca tttccgacct tgcatggaaa aaacatcaga tgccagcatg
     2581 gcatttaatt accgccgatg gtcagggtat tactctagtg aatattggcg tgggaccgtc
     2641 aaatgctaaa accatctgcg atcatctggc agtgctacgc ccggatgtct ggttgatgat
     2701 tggtcactgt ggcggattac gtgaaagtca ggccattggc gattatgtac ttgcacacgc
     2761 ttatttacgc gatgaccacg ttcttgatgc ggttctgccg cccgatattc ctattccgag
     2821 cattgctgaa gtgcaacgtg cgctttatga cgccaccaag ctggtgagcg gcaggcccgg
     2881 tgaggaagtc aaacagcggc tacgtactgg tactgtggta accacagatg acaggaactg
     2941 ggaattacgt tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga
     3001 tatggaaagt gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact
     3061 actgtgtgtt tcagataaac cgttgcatgg cgagattaaa cttcccggcc aggctaaccg
     3121 tttttatgaa ggcgctattt ccgaacatct gcaaattggc attcgggcga tcgatttgct
     3181 gcgcgcagaa ggcgaccgac tgcattcgcg taaattacga acctttaatg agccgccgtt
     3241 ccgataataa gttctgaacc ttcttatcag aaatagatgt gaaggagtaa gtaagacacc
     3301 tggcaaatag cctgcaataa tcgtgggctg tttgcttcct tgggcggata cgtgttttat
     3361 tatcgtctta atgatttcca catattaaaa gcaagtatgc tt
//
LOCUS       DAZGTR010000254         3385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGTR010000254 DAZGTR010000000
VERSION     DAZGTR010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.256"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            128..292
                     /locus_tag="ACHLCY_004955"
     CDS             128..292
                     /locus_tag="ACHLCY_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP0372016.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            261..404
                     /gene="kil"
                     /locus_tag="ACHLCY_004956"
     CDS             261..404
                     /gene="kil"
                     /locus_tag="ACHLCY_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP0372017.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILMLKLRSSK
                     SIH"
     gene            480..776
                     /locus_tag="ACHLCY_004957"
     CDS             480..776
                     /locus_tag="ACHLCY_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0372018.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            782..1567
                     /gene="bet"
                     /locus_tag="ACHLCY_004958"
     CDS             782..1567
                     /gene="bet"
                     /locus_tag="ACHLCY_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP0372019.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKASEQKVAA"
     gene            1564..2244
                     /locus_tag="ACHLCY_004959"
     CDS             1564..2244
                     /locus_tag="ACHLCY_004959"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0372020.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARVLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYLAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2241..2423
                     /locus_tag="ACHLCY_004960"
     CDS             2241..2423
                     /locus_tag="ACHLCY_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP0372021.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            2396..2587
                     /locus_tag="ACHLCY_004961"
     CDS             2396..2587
                     /locus_tag="ACHLCY_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP0372022.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            2664..2879
                     /locus_tag="ACHLCY_004962"
     CDS             2664..2879
                     /locus_tag="ACHLCY_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0372023.1"
                     /translation="MTPQQENALRSIARQTNSEIKNARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2978..3199
                     /locus_tag="ACHLCY_004963"
     CDS             2978..3199
                     /locus_tag="ACHLCY_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0372024.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASFQEDLELISKQRGSK"
     gene            3196..>3385
                     /locus_tag="ACHLCY_004964"
     CDS             3196..>3385
                     /locus_tag="ACHLCY_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0372025.1"
                     /translation="MSEINYQALREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESD"
BASE COUNT          958 a          786 c          850 g          791 t
ORIGIN      
        1 aaacaggagc cgacttgttc tgattattgg aaatcttctt tgccctccag tgtgagggcc
       61 tttttatatg cataccaata acgcttcact cgaggcgttt tcgttatgta taaataagga
      121 gcacaccatg caatatgcca ttgcagggtg gcctgttgct ggctgccctt ccgaatcttt
      181 acttgaacga atcacccgta aattacgtga cggatggaaa cgccttatcg acatacttaa
      241 tcagccagga gtcccaaaaa atggatcaaa cacttatggc tatccagact aaattcacta
      301 tcgccacttt tattggcgat gaaaagatgt ttcgtgaagc tgtcgacgct tataaaaaat
      361 ggatattaat gctgaaactg agatcaagca aaagcattca ctaaccccat ttcctgtttt
      421 cctaatcagc ctggcatttc gcgggcgata ttttcacagc cattttcagg agttcagcca
      481 tgaacgctta ttacattcag gatcgtcttg aggctcagag ctgggcgcgt cactaccagc
      541 agatcgcccg tgaagagaaa gaggcagaac tggcagacga catggaaaaa ggcctgcccc
      601 agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg ccacggggcc agcaaaaaag
      661 ccattacccg tgcgtttgat gacgatgttg agtttcagga gcgcatggca gaacacatcc
      721 ggtacatggt tgaaaccatt gctcaccatc aggttgatat tgattcagag gtataaaacg
      781 gatgagtaca gcactcgcaa cgctggcagg gaagctggct gaacgtgtcg gcatggattc
      841 tgtcgaccca caggaactga tcaccactct tcgccagacg gcatttaaag gtgatgccag
      901 cgatgcgcag ttcatcgcat tgctgatcgt cgccaaccag tacggtctta atccgtggac
      961 gaaagaaatt tacgcctttc ctgataagca gaacggcatc gttccggtgg tgggcgttga
     1021 tggctggtcc cgtatcatca atgaaaacca gcagtttgat ggcatggact ttgagcagga
     1081 caatgaatcc tgtacatgcc ggatttaccg caaggaccgt aatcatccga tctgcgttac
     1141 cgaatggatg gatgaatgcc gccgcgaacc attcaaaacc cgcgaaggca gagaaatcac
     1201 cggaccgtgg cagtcgcatc ccaaacggat gttacggcat aaagccatga ttcagtgtgc
     1261 ccgtctcgcc ttcggatttg ctggtatcta tgacaaggat gaagccgagc gcattgtcga
     1321 aaataccgca tacactgcag aacgtcagcc ggaacgcgac atcactccgg ttaacgatga
     1381 aaccatgcag gagattaaca ctctgctgat tgccctggac aaaacatggg atgacgactt
     1441 attgccgctc tgttcccaga tatttcgccg cgacattcgc gcatcgtcag aactgacaca
     1501 ggccgaagca gtgaaagctc ttggattcct gaaacagaaa gcctctgaac agaaggtggc
     1561 tgcatgacac cggacattat cctgcagcgt accgggatcg acgtgagagc tgtcgaacag
     1621 ggggatgatg cgtggcacaa attacggctc ggcgtcatta cagcttcaga agttcacaac
     1681 gtgatagcaa aaccccgctc aggaaagaaa tggcctgaca tgaaaatgtc ctacttccac
     1741 accctgcttg ctgaggtttg caccggtgtg gctccggaag ttaatgccaa agcactggcc
     1801 tggggaaaac aatacgagaa cgacgccaga gtcctgtttg agttcacctc cggcgtgaat
     1861 gttactgaat ccccgattat ctatcgcgac gaaagtatgc gtacagcctg ctctcccgat
     1921 ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc cgtttacctc ccgggatttc
     1981 atgaaattcc ggctcggtgg tttcgaggcc ataaaatcgg cttacctggc ccaggtgcag
     2041 tacagcatgt gggtgacgcg aaaagatgcc tggtactttg ccaactatga cccgcgtatg
     2101 aagcgtgaag gactgcatta tgtcgtggtt gagcgggatg aaaagtacat ggcgagtttt
     2161 gacgagatgg tgccggagtt catcgaaaaa atggacgagg cactggctga aattggtttt
     2221 gtatttgggg agcaatggcg atgaagcatc ctcacgataa tatccgggta ggcgcgatca
     2281 ctttcgtcta ctccgttaca aagcgaggct gggtatttcc cggtctttct gttatccgaa
     2341 atccactgaa agcacagcgg ctggctgaag agataaataa taaacgaggg gctgtatgca
     2401 caaagcatct cctgttgaat taagaacgag tatcgagatg gcacatagcc ttgctcaaat
     2461 tggagtcagg tttgtgccaa taccagtaga aacagacgaa gaatttcata cgttagccgc
     2521 atccctttca caaaagctgg aaatgatggc ggcgaaagca gaagcaaacg agagagaccc
     2581 ggcatgacaa caacagaatg catttttctg gcagcgggct tcatattctg tgtgcttatg
     2641 cttgccgaca tgggacttgt tcaatgacac ctcagcaaga aaacgccctt cgcagcattg
     2701 cccgtcagac taattctgaa atcaaaaatg ccagacagca gtttccggat aaaaacgtcg
     2761 atgacatttg ccgtagcgta ctgaagaagc accgcgaaac ggtaacgctg atgggattca
     2821 caccgactca cttaagtctg gcaatcggta tgttaaacgg cgtctttaag gaacggtgaa
     2881 catgaaaagc aaaatcatca gggagctaca ggctcctttt ttattattcg cattcaccct
     2941 caagcgtatt aaccaacaat tcagggatta atgaaagatg gcagatatca ttgattcagc
     3001 atcagaaatt gaagaattac agcgcaatac agcaataaaa atgcgtcgcc tgaactacca
     3061 gactgtatcc gcaactcatt gttgtgagtg tggcgatccg atagatgaac gaagacgcct
     3121 ggcagttcag ggttgtcgga cttgtgcaag tttccaggag gatctggagc ttatcagtaa
     3181 acagagaggt tcgaagtgag cgaaattaat tatcaggcac tgcgtgaaaa ggcagaaaaa
     3241 gcaactaaag gaagctacat cgtagggcat acatctgtta accagcacgg caatttaaca
     3301 ggagtttttg tttgccaaaa atggaaagga gaacccggtg gcgtgattgc ggaatgtcat
     3361 gttaactgcc tgattgaatc agatg
//
LOCUS       DAZGTR010000255         3320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGTR010000255 DAZGTR010000000
VERSION     DAZGTR010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.257"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            68..358
                     /locus_tag="ACHLCY_004965"
     CDS             68..358
                     /locus_tag="ACHLCY_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372026.1"
                     /translation="MTGKEIILEYLKTHEQFSPHELALITGIPNNRIAQAARHMVKQG
                     HLSVVERKWKTVIYAKRKVKKEPIKRNPDGTGWGCANPMTAFINRALMEVRQ"
     gene            431..637
                     /locus_tag="ACHLCY_004966"
     CDS             431..637
                     /locus_tag="ACHLCY_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372027.1"
                     /translation="MAELIFSALRILGAMWMVATFIVVASSFVRLVGEGKDLVGVLFG
                     SIFLWVIIGVMPVAVAKMAWSFVS"
     gene            655..1017
                     /locus_tag="ACHLCY_004967"
     CDS             655..1017
                     /locus_tag="ACHLCY_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372028.1"
                     /translation="MDESRKQFEESWLRRGGESSDLIRYPENHHEIGSGDIGGQYVMD
                     DVQGHWQTWQASRAAIELDIDWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIR
                     AAGIKEKNFDEANNLPPN"
     gene            989..1399
                     /locus_tag="ACHLCY_004968"
     CDS             989..1399
                     /locus_tag="ACHLCY_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP0372029.1"
                     /translation="MKQTIFLRTKQQQQAAINAILATPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVFPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGHGVRFTDRRYE"
     gene            1396..1572
                     /locus_tag="ACHLCY_004969"
     CDS             1396..1572
                     /locus_tag="ACHLCY_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0372030.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            1575..1934
                     /locus_tag="ACHLCY_004970"
     CDS             1575..1934
                     /locus_tag="ACHLCY_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein NinX family protein"
                     /protein_id="HFP0372031.1"
                     /translation="MDYSQLSDFEINVAVFEAIHNGSPDYKEGENGDMVFVSFEGDIV
                     NGDAVEVEVERGSFNPCANPADSWPIIEKYRISIINLDEDEWGARGVAYCKSKRAIHE
                     NPLRAAMIVFLMMQRIQ"
     gene            1934..2110
                     /locus_tag="ACHLCY_004971"
     CDS             1934..2110
                     /locus_tag="ACHLCY_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP0372032.1"
                     /translation="MLSPSQSLQYQKESVERALTCANCGQKLHVLEVHVCEHCCAELM
                     SDPNSSMYEEEDDE"
     gene            2103..2315
                     /locus_tag="ACHLCY_004972"
     CDS             2103..2315
                     /locus_tag="ACHLCY_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372033.1"
                     /translation="MSDYLKWYLCHRWLIKFAVKDWMTADANKLKQRKDYYYARMKEN
                     YCSIRTRIFIKKDLQSILQLRGKVNG"
     gene            2308..2598
                     /locus_tag="ACHLCY_004973"
     CDS             2308..2598
                     /locus_tag="ACHLCY_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0372034.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSDCHAEIDRRTRILDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            2595..2957
                     /locus_tag="ACHLCY_004974"
     CDS             2595..2957
                     /locus_tag="ACHLCY_004974"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0372035.1"
                     /translation="MNEYQFVLPYPPSVNTYWRRRGSQYYISDKGQKYRKDVQQIIHQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRIIRG
                     VKVPGGRLGIKITELENA"
     gene            2954..3142
                     /locus_tag="ACHLCY_004975"
     CDS             2954..3142
                     /locus_tag="ACHLCY_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFP0372036.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            3139..3210
                     /locus_tag="ACHLCY_004976"
                     /pseudo
     CDS             3139..3210
                     /locus_tag="ACHLCY_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
BASE COUNT         1001 a          696 c          854 g          769 t
ORIGIN      
        1 agtttagtct ccaggatttc cggggcggtt cacttgcgtt tacatgggat agttacagga
       61 gcagatcgtg actggaaaag aaatcatcct ggaatatctg aaaactcatg aacaattctc
      121 cccacatgaa ttagcactga tcaccggaat accaaataac agaatcgctc aagcagcaag
      181 gcatatggtg aaacaaggac atttgagtgt tgttgagcgt aagtggaaga cggttattta
      241 tgcaaaacgc aaagtgaaga aggagccaat taaaagaaat ccagatggta cggggtgggg
      301 atgtgcaaat ccaatgacgg cgtttattaa tagggcgctt atggaggtaa ggcaatgacc
      361 atctacatca ctgagctaat aacaggcctg ctggtaatcg caggcctttt tatttggggg
      421 agggtaaatc gtggctgagt taattttctc tgcattgagg attctcggtg ctatgtggat
      481 ggtggcgacg ttcattgttg ttgccagcag ttttgtccgg ttggtaggcg aaggtaaaga
      541 cctggtgggt gtgcttttcg gtagcatttt cctgtgggtg attatcggtg ttatgcctgt
      601 cgctgtagca aaaatggcgt ggagttttgt gagttgaact gagggtaagt atcgatggac
      661 gaatcaagaa agcagtttga agaaagttgg ttgcgacgtg gaggcgaatc ctcagacctt
      721 atccgttacc ctgaaaatca ccatgaaatt ggcagtggtg atattggtgg tcaatacgtg
      781 atggacgatg ttcaaggcca ctggcaaacg tggcaggcat cgcgagcagc tattgaactg
      841 gatattgact ggcccgaatc gaatgacgac ttttggaaag atggtgaaga aggtgcttat
      901 gcgatgggtt atgaggatgg gagagacaaa acggtaattg cagtaatgaa agctatcaga
      961 gccgctggaa ttaaagagaa gaatttcgat gaagcaaaca atcttcctcc gaactaagca
     1021 acaacagcaa gctgcaataa atgccatcct cgcaacacca ctcgataaag acaagccagt
     1081 caccatccgc attactgact acaagcgcaa ccttgaccag aacgcaaaat ttcacgcgat
     1141 gctggcggat atcgctcgtc aggttcaatg gtgcggcaaa tggttaaaac cagaacaatg
     1201 gaaggttttg ttgatcagcg gtcatgcagt ggcaacaaaa caggaagctg atgtttttcc
     1261 cgggcttgaa ggcgaatacg tcaacattcg cgaaagcagc gcgcagatga gtgtgaagcg
     1321 tatggcaagt cttatcgagt acacaacagc ctgggctatt ggtcatggtg tcagatttac
     1381 cgacaggagg tacgaatgag acgacagcga cgaagtatca ccgacatcat ctgcgaaaac
     1441 tgcaaatacc ttccaacgaa acgctccaga aataaacgca agccaatccc aaaagaatct
     1501 gacgtaaaaa ccttcaacta cacggctcac ctgtgggata tccggtggct aagacatcgt
     1561 gcgaggaaat gacaatggat tattcacagt taagtgattt tgaaattaac gtggcggtat
     1621 tcgaagccat tcataacgga tcgccggatt acaaagaagg tgagaatggc gatatggtgt
     1681 ttgtctcatt tgagggagac attgtaaacg gagacgcagt tgaagtagaa gttgagcgcg
     1741 gatcctttaa cccatgcgca aacccagcag actcatggcc gattattgaa aaatacagga
     1801 ttagcattat caatctcgat gaagacgagt ggggtgcacg cggtgtggcc tactgtaaat
     1861 ctaagcgagc tatacatgaa aatccccttc gcgccgccat gattgtcttt ctcatgatgc
     1921 agagaatcca ataatgctta gcccatccca atcccttcaa taccagaaag aaagcgtcga
     1981 gcgagcttta acgtgcgcta actgcggtca gaagctgcat gtgctggaag ttcacgtgtg
     2041 tgagcactgc tgtgcagaac tgatgagcga tccgaatagc tcaatgtacg aggaagaaga
     2101 cgatgagtga ttacctgaaa tggtatctct gccaccgctg gctaattaag tttgctgtaa
     2161 aagactggat gacagcggat gccaacaaac ttaagcaaag aaaggactat tactacgcca
     2221 gaatgaagga aaactactgc tcaattcgca ctcgcatatt tattaaaaaa gaccttcagt
     2281 caattcttca attgcgaggg aaggtaaatg gctaacctac gcaaagaagc gcgcggcaga
     2341 gaatgccagg tacgtattta cggcgtatgc aatggcaatc ctgaaactac agttctggca
     2401 cattaccgga tggctggaat ttgtggaacg gggatgaagc ctgacgacct gatcggcgca
     2461 tgggcttgta gtgactgcca cgcggagatc gaccgacgca caaggattct cgacaacaaa
     2521 gacgccagac tttaccacct cgaaggcgtg atcaggacgc aggcgatact gctgaaggag
     2581 gggaagatta agtcatgaac gaatatcagt ttgtgcttcc atacccgccg tcggtgaata
     2641 cctactggcg aagacgggga agccaatact acatcagcga taaaggccag aaataccgaa
     2701 aagacgttca gcaaatcatc caccaactca agttagatat tttcaccaaa tcacgactcc
     2761 gcatcaaagt aatcgcagac gttccagact cccgccgccg cgacctagat aacatcctga
     2821 agggtttact cgactccctt atccatgccg gatttgcgga agacgacgag caattcgatg
     2881 acattcgcat aattcgtggt gtgaaagtac caggcggacg gcttggaata aaaatcaccg
     2941 aactggagaa cgcatgaacg ccacaattca aacgatacca gagcttctta tccagacacg
     3001 aggcaatcag accgaagtgg cgaggatgct ttcctgcgca agaggaacag tgctcaagta
     3061 caaccgagac agcaaaggcg agcgtcatgt aatagttaac ggcgtcctga tggtcaaaca
     3121 gggcaagagg ggaagacgat gagactcgaa agcgtagcta aatttcattc gccaaaaagc
     3181 ccgatgatga gcgactcact actggccaca gtttattggt tttcgtaact gagtcatttt
     3241 attattttat tgcaactttt aatcttttat agtgcgaaat aaatggagct ggcattcatt
     3301 tcgcacttta tgtttttgtt
//
LOCUS       DAZGTR010000256         3238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGTR010000256 DAZGTR010000000
VERSION     DAZGTR010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.258"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            46..630
                     /locus_tag="ACHLCY_004977"
     CDS             46..630
                     /locus_tag="ACHLCY_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0372037.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            710..1405
                     /locus_tag="ACHLCY_004978"
     CDS             710..1405
                     /locus_tag="ACHLCY_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0372038.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1435..>3238
                     /locus_tag="ACHLCY_004979"
     CDS             1435..>3238
                     /locus_tag="ACHLCY_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0372039.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDG"
BASE COUNT          941 a          750 c          725 g          822 t
ORIGIN      
        1 cacattaaat atgattatgt acttgttaca aggataaggt tataaatgaa taaagttaca
       61 aaaacagcta ttgtgggctt acttgcgctt ttcgcaggta atgccgctgc aaccgatggt
      121 gaaatagttt ttgatggcga gattttaaaa tccgcttgtg aaatcaatga ctctgataag
      181 aaaattgaag ttgctcttgg tcactataat gccgaacagt ttcgttctgt tggcgatcgt
      241 agcccaaaaa ttccattcac tattccttta gttaactgcc cagtgactgg gtgggagcac
      301 gataacggca acgttgaagc gtctttccgt ttatggctgg aaacgcggga taatggcacc
      361 gtccctaact tccctaatct ggcaaaagtg ggctcttttg ctggcacagc ggcaacaggt
      421 gtgggtatcc gtattgacga tgcggaaagc ggaaacctta tgccactgaa tgctatgggg
      481 aatgataaca cggtttatca gatcccagcg gactccaatg gtattgtcaa tgttgacctc
      541 atcgcttact acgtatcaac cgttgaagca tcagaaatca ccccagggga agcagacgct
      601 gtcgttaacg taacgctgga ttaccgttaa tacgttacgg cgttatctga cctttcagat
      661 aacgcccttt tccttcctct ttctcgttgt atcaggttga aaaataacta tgtcaaaacg
      721 aacattcgcg gtgataataa ccttgttgtg tagcttctgt atcggccagg cgcttgcagg
      781 gggtatcgtt ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag aggctgcgtt
      841 acctgtcgca aacaaaggcg cagaaacgcc ttatttactg caatcatggg tagataatat
      901 agatggtaca agccgtgccc catttattat aaccccaccg ctatttcgtc ttgaagccgg
      961 cgatgactca tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc
     1021 gctgttctac attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc
     1081 taacgagttg acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct
     1141 gaagggacgg gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact
     1201 caatatatat aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac
     1261 cgatctcaca agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc
     1321 tgcatctggc ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg
     1381 gacagaaact cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca
     1441 caacgacacc accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgc
     1501 tgtttgccga tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag
     1561 tattatttcg accccattat gctggaaact acaaaaagtg gtatgcaaac aactgatctg
     1621 tcacgttttt caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg
     1681 aataaaaaga aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg
     1741 cagccacagt ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg
     1801 gcgctggctg aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt
     1861 acagctgctg aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca
     1921 ctgtaccgtg atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg
     1981 ctgtttacca actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc
     2041 caacgacagt acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgtaac
     2101 tattctacgt ggacacgcaa cgatcaggcg tcaagctgga acactatcag tagttattta
     2161 caacgtgata tcaaggcgtt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc
     2221 agtatttttt ccagctacac ctttactggc gtgcaactcg cttccgacga taatatgttg
     2281 ccaaacagcc agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc
     2341 gtgactatca ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt
     2401 gaaattaacg atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa
     2461 agtgacggta cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga
     2521 cctgggcatc taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat
     2581 agcaaggaac ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg
     2641 ctgtatggcg gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc
     2701 acacttggcg cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat
     2761 aaccggcact cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa
     2821 accaacacca atatcgctgt tagctactat cgctatacca acgatggcta ttttagtttt
     2881 gatgaagcca atacccgtaa ttgggactat aacagtcgcc aaaaaagtga aattcaattc
     2941 aacatcagcc agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac
     3001 tattggggca ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg
     3061 ggaattggtt acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc
     3121 gcactctctt tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc
     3181 tatcagatga ccagccagaa agatcgccca acccaacatg aaatgcgtct tgatggct
//
LOCUS       DAZGTR010000257         3200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGTR010000257 DAZGTR010000000
VERSION     DAZGTR010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.259"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..672
                     /locus_tag="ACHLCY_004980"
     CDS             <1..672
                     /locus_tag="ACHLCY_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0372040.1"
                     /translation="ATIFTFSFGFILALALAKKNIRFAKVWRLIFILPYAIPTFVTLL
                     IFRLLLNGIGPVNSFLNSIGMNSVDFLSVPLNAKITVIAVCVWVGAPYFMLLIAGALT
                     NISRDLYEASEVDGATKWQQFKEITLPMVLHQVAPTLVMTFAYNFNNFGAIYLLTEGG
                     PTNPEYRFAGHTDIFITWIYKLTLNFQQYHIASVISIIIFIFLSVIAIWQFRRMKSFK
                     DDVGM"
     gene            675..1508
                     /locus_tag="ACHLCY_004981"
     CDS             675..1508
                     /locus_tag="ACHLCY_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP0372041.1"
                     /translation="MDKFLRKISTLLVYLFLICNSILVLGPVIWTIMASFKKGNNLFS
                     STFSGIEFTFDHYITLFTDTPYMQWYLNTFILATANMLISLFVVTMTAYVFSRYRFRG
                     KRNTLMSILVLQMFPAFLSMTAIYILLSKANLIDTYTGLLLVYVTGSLPFMTWLVKGY
                     FDAIPTSLDEAAKIDGAGHLTIFIQIILPLAKPILVFVGLVSFTGPWMDFILPTLVLR
                     SEDKMTLAIGIFSWIASNSAENYTLFAAGALLVAVPITLLFVFTQKHITTGLVSGAVK
                     E"
     gene            1528..>3200
                     /locus_tag="ACHLCY_004982"
     CDS             1528..>3200
                     /locus_tag="ACHLCY_004982"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP0372042.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTEAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPDCEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEGVEPWGSAPTSFNFMGGDLWGVIDKLDYLEELGINGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIKPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCVASIQWKDVEHPTVIAYQRD"
BASE COUNT          912 a          545 c          680 g         1063 t
ORIGIN      
        1 gcgacgattt ttaccttcag ttttggcttt attctggctt tagcactggc taaaaaaaat
       61 atacgtttcg caaaagtctg gcgattgata tttatactgc catatgccat acccacattt
      121 gtaacgttac ttattttccg tcttctttta aatggaattg gtccggttaa ctcgtttctg
      181 aacagtatcg gaatgaattc agttgatttt ctgtcggttc cattaaatgc aaaaataaca
      241 gtaattgccg tttgtgtttg ggtaggggca ccgtatttta tgctattgat cgcgggtgca
      301 ttaacaaaca tatcccgaga tttgtatgag gctagtgagg tcgatggcgc aactaaatgg
      361 caacagttta aggaaattac attgccaatg gtgttacatc aggttgcgcc aactctggta
      421 atgacatttg catataattt caataatttt ggtgcaattt atcttctgac tgaaggtggt
      481 ccgactaatc cggaatacag attcgctggg cataccgata tttttattac ctggatttat
      541 aaactcacat tgaatttcca gcaatatcat atagcttctg taatctccat cataatattt
      601 atcttcttat cggttatagc aatatggcaa ttcagacgga tgaaatcatt taaagatgat
      661 gtagggatgt gacaatggat aaatttttac gtaaaatatc aacgctctta gtttatcttt
      721 ttttaatctg caactccatt cttgtgcttg ggccagtgat atggacgata atggcatctt
      781 ttaaaaaagg gaataatcta tttagttcaa catttagtgg catcgagttt acatttgacc
      841 actacataac gttgttcacg gatacgccat atatgcagtg gtatttgaac actttcattc
      901 ttgctaccgc taacatgtta atttcactgt tcgttgtgac gatgaccgcc tacgtttttt
      961 caaggtatcg atttcgtgga aagcgtaata ctttaatgag tattcttgta ttgcaaatgt
     1021 ttcctgcatt tttatcaatg actgcaattt atatcctgct atcaaaggcc aatttgatag
     1081 acacatatac tgggttgctt ctggtttatg taaccggttc attgcccttc atgacgtggt
     1141 tggtaaaagg ttattttgat gctattccaa cttctttgga tgaggccgct aaaatagatg
     1201 gagcagggca tctgactatt tttattcaga taatattgcc attggctaag ccgattctcg
     1261 ttttcgttgg attagtttcg ttcactggac catggatgga cttcatcttg ccaacgttgg
     1321 tgcttcgtag tgaggataaa atgaccctgg cgattggaat ttttagttgg attgcttcta
     1381 attcagcaga gaattatacg ctttttgctg ctggggcgct tttagttgca gtacctatca
     1441 cattgttatt tgtattcaca cagaagcata taactactgg acttgttagc ggtgccgtta
     1501 aggaataatt tttagggatt agtatttatg attacagtta gttcgttaaa acactcagca
     1561 aaaagtgaag atagctatgc ctatgataac gaaactttgc atgtacggtt acgcacatta
     1621 agaggagaag ttgataaagt tattctgtgg ataggcgatc catataattg ggctgaaggt
     1681 ggtcttgacg gcggtaacat ggccgggaca gaagcgttcg gctggattgg cggcaacgag
     1741 atcgagatgg aacaagaagc tgtcaccgaa tttcatgatc actggtttgc agtgttcaaa
     1801 cctcaaaaac gccgttgccg ttacggtttt atactttttg ggaaagaggg agagaagttt
     1861 ctttttggtg agaaaaggtg tgttgatatc agttcaccgg actgcgaaga acgagagtta
     1921 agccgcttaa ataacttttt ttgcttccct tatcttaata aaattgacgt tctaaatacc
     1981 ccctcctggg taaaaaatac ggtatggtac caaatttttc cagaccgttt ctgtaacggc
     2041 agacctgaaa tatccccaga aggggtagaa ccgtggggca gtgcaccgac ttcattcaac
     2101 tttatgggtg gggatctgtg gggggttatt gataaactcg attatctgga agaactgggt
     2161 attaatggga tttatttttg tcctattttt acctcagctt ccaaccataa atatgacacc
     2221 atcgatcatt ttagtgttga tccacattta ggtgggaata ttgctttcca tactttgatt
     2281 gaagaagcgc ataagcgtgg aataaaaatc atgttggatg cagtattcaa tcatttaggc
     2341 gctgactctc caatatggct tgacgttgta cgcaatggtg ccaattcacg ttacgccgat
     2401 tggttctgga ttcataaatt tcctgtttat cccgatactc caaaaagtga atgggatttt
     2461 aaaaacttta actatgaaac ctttggcaat gtaattgaaa tgcccaagct aaatactgag
     2521 aatgaagagt gccgtgaata cttacttagt atagttcgct attggacgca aaattttgat
     2581 attgatggtt ggcgtctgga tgttgcgaat gaagtggatc atcatttctg gcgagatttc
     2641 agaaaggtga ttaaagatat caaacctgaa tgttatattt tgggtgagat atggcatgag
     2701 ggcaccccct ggttgcgtgg tgatcagttt gattcattaa tgaattatcc cttaacatat
     2761 gggattattg attattttgc gttacaggat acaacaaaac aagaatttat gacatccgtt
     2821 acacgttctt atctttgtta tcctaaaaat ataactgaag ttatgtttaa tcttctggat
     2881 agtcatgaca ccgcgcgtat tttaagcgtt tgtttgaatg ataaacgaaa agttaaactc
     2941 gcctatttat ttatgttaac tcaagcgggc tctccttgta tctactacgg tagcgaaata
     3001 ggaattgatg gttttaaatc aatgacgctt gagaataata gaaaatgtat gatatgggat
     3061 gaaaacaaac aagatcttga attacggcaa tttatacgtt ggttaatacg tctacgtaaa
     3121 aaacatccac aatggtgtgt ggcatcaata caatggaaag atgttgaaca tcctacggtg
     3181 atcgcttatc aacgcgataa
//
LOCUS       DAZGTR010000258         3088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGTR010000258 DAZGTR010000000
VERSION     DAZGTR010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.260"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(197..364)
                     /locus_tag="ACHLCY_004983"
     CDS             complement(197..364)
                     /locus_tag="ACHLCY_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0372043.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(361..792)
                     /locus_tag="ACHLCY_004984"
     CDS             complement(361..792)
                     /locus_tag="ACHLCY_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0372044.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(962..1038)
                     /locus_tag="ACHLCY_004985"
     tRNA            complement(962..1038)
                     /locus_tag="ACHLCY_004985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1002..1004),aa:Arg,seq:tct)
     gene            complement(1139..1214)
                     /locus_tag="ACHLCY_004986"
     tRNA            complement(1139..1214)
                     /locus_tag="ACHLCY_004986"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1179..1181),aa:Ile,seq:cat)
     gene            complement(1354..1908)
                     /locus_tag="ACHLCY_004987"
     CDS             complement(1354..1908)
                     /locus_tag="ACHLCY_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP0372045.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPKLEAFLRDMINGKQKSWLVHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(1905..2195)
                     /locus_tag="ACHLCY_004988"
     CDS             complement(1905..2195)
                     /locus_tag="ACHLCY_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0372046.1"
                     /translation="MVNLRKAAKGQICQIRIPGYCNHNPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACNSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKDGYL"
     gene            complement(2195..2794)
                     /locus_tag="ACHLCY_004989"
     CDS             complement(2195..2794)
                     /locus_tag="ACHLCY_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFP0372047.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDEV
                     EFQQVYKSVLNVLWNWILFRKFSSQEEVENVAAQLLEFA"
BASE COUNT          769 a          856 c          682 g          781 t
ORIGIN      
        1 cccataaaaa agcccctccg gagaggggca tgtttgcatg cacattcttt ttcttgcatg
       61 gtgccgggtg cctcccggtg aattcagtat cagcacctga atccgcgatt atcccatata
      121 cctggttgct gatcgcccct ccgcacaggg ggattcacca tgcagaagtg tttttaataa
      181 acagcaaaca aaaaaatcaa gcattatgca ggctgtttct ttttatcacc ggccacagca
      241 ataccacaat gccgcagacc agcaccccat ccgccagcac cgacatgatt ctgctggtga
      301 aatccaccat caccaccaga aacagcagga gtgcagccac agccaggcgc agttttaccg
      361 tcacaggtga ttctccagac gaagacccag aacaccggca atctcttcca gcaccttgcg
      421 ctcttccggc tcaatttcgc cgtctgcctc cgcaatggcc accgccacat ccagcacatc
      481 ttccgcttca cgcgtatcgt gtttcacatc ctcaatctcg cgtaacgcgg cacgacgacc
      541 aattttaaaa ttggtatcca gctgaccgat aatagttgcg ctaatcgcat taatttccga
      601 ggtaaacgca gacaacgcag gctgattacg taagacctgt tcgatcttcg ctttctcgga
      661 tgcctcgcat tcaccatctg cataggccac caggtatgca gcgttaatca ccgcctgtgc
      721 cagatcgcgt ttctcaaact ttttaatttc cgctgccgct ctgcgggttt tctttttgaa
      781 gattccaaac atcgtgacgt tcctttgggt gggtgagcca acgcccggga gcgatctgcc
      841 cacagagaaa gtcacactga ccactccgta agctcacccc cgaaaggctc tgtggttgat
      901 atgcgccggg cgtggcgcag atacaaaaaa ggcccgccga agcgagcctg gaaaataagt
      961 gtggcgcgtt gtagtggagt cgaaccactg accgattgct tagaaggcaa ttgctctgtc
     1021 cggctgagct aacaacgcag aataccgata atggaccgcc atcggggacc cgcccccgca
     1081 ccaacaaccc tgttatggtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     1141 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1201 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaattccgc aataaaaaac
     1261 ccgctcaatg gcgggttctg gtaaagttca tgcgtttggt tcgcctcgcg atacagcttt
     1321 gcgaagctta ccggaattga agcagttttt acgtcaaaaa gcaataactt ttttctctat
     1381 accaaaagcc ataaccattg gtttgtacaa aataaattct gccaccttta gccaatgctc
     1441 aatgcgtctt tcacaggttc ttaaactcca ttccggatgt gcatcattca gcagttcagc
     1501 cattttgcgc ttagtcatcc cccgccccac ataacgctga ctcagaacat tgagcagccc
     1561 gggataacct gccagtactt caccaataac cctgtcgatt attaacgcct ctgaatcggt
     1621 acaatgcacc agccagcttt tttgcttgcc gttgatcata tcccgcaaaa aagcctcaag
     1681 tttaggtttg tccagacctg cttttttcat cctccggagc gcctcgttaa ttgccgtttt
     1741 tgtcagcttt ttagaggtca gcaactggtt gaacatattt cccgtcttac cgccgccaat
     1801 atacgaccag cgcccccaca tacgtagttt cccctgaatc cagacacttt ccagcgtggt
     1861 gagacgaagg tgctctccgc tttttcctgt gttcgttggg taaatcataa ataaccgtcc
     1921 tttctccaga tctcttgtgt gcgaaaaaca ccttctgcat gcatcaggcg caattcttct
     1981 ttggtgtaat cgctggtttt tactctcccg tcgattaaat cgtggcatga gttacaggca
     2041 atcgctgcct gcatatcgtg tggctttatc gctgttccgc acgttcccgc cagcctgtaa
     2101 tgcgccagca cagaggtttc gggattgtga ttgcagtagc cagggattct gatctggcat
     2161 atctggcctt tagccgcttt acgtaaattc accattacgc aaactccagt agttgtgcgg
     2221 ccacattttc aacttcctcc tgagaggaga atttacggaa cagaatccag ttccacagca
     2281 cattcagtac agatttataa acctgctgaa actcgacttc gtccatattc gcaaaagcga
     2341 tggattttgc ccgacgccca cggctaccgt ccggataaat atgctcggtg taaaatccgg
     2401 cctgaatggt tacccactcg cggaaagcct caaacgactt tagcaacgcc gtatcccggg
     2461 ttctacgagt cgcaacggtg ttaaggtatt gctctgcggc atcactcagg gctggcgtgt
     2521 gttcccgacc aactgattcg cacaggtaat caacgaaacc agacagcagt tctcgttcgc
     2581 gaggcgtgat cgccccaccg accggagtcc agtaatcgaa tcccagttgc aggagtttga
     2641 aaaaacgctt gtggaatgcg tagttacgca cacgcttaaa atctgcgtgt atccactcac
     2701 ctattttgat ttgatgcaga aaatcgcaac tctccggcgt cgccgggaga agtaaaccag
     2761 aagaagtttg tttgaccagt tgtatatgcg ccatttctca atctctcgat ggcgcagtgc
     2821 agcagatgcc agttgttcaa gctagcacgc aaattgtaaa ccagaatgcc aggaaaaaac
     2881 aaaacccgcc gaagctggtt gtgtgcggtt gcgttgaagt ctcctagcca aagagtagcg
     2941 gtgaccactc cccgccgata atcttagttg tttaatttcg taagctaact aggctaaaag
     3001 ctctctgttg tctgcctgtt gtacaattaa caaaatgaac aacgaactag atataccaac
     3061 gccagaatgg tgcaacaaat cccaaaca
//
LOCUS       DAZGTR010000259         3074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGTR010000259 DAZGTR010000000
VERSION     DAZGTR010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.261"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..758
                     /locus_tag="ACHLCY_004990"
     CDS             117..758
                     /locus_tag="ACHLCY_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0372048.1"
                     /translation="MPVDLTPYILPGVSFLSDIPQETLSEIRNQTIRGEAQIRLGELM
                     VSIRPMQVNGYFMGSLNQDGLSNDNIQIGLQYIEHIERTLNHGSLTSREVTVLREIEM
                     LENMDLLSNYQLEELLDKIEVCAFNVEHAQLQVPESLRTCPVTLCEPEDGVFMRNSMN
                     SNVCMLYDKMALIHLVKTRAAHPLSRESIAVSMIVGRDNCAFDPDRGNFVLKN"
     gene            complement(1339..1773)
                     /gene="uspF"
                     /locus_tag="ACHLCY_004991"
     CDS             complement(1339..1773)
                     /gene="uspF"
                     /locus_tag="ACHLCY_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP0372049.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1914..3047)
                     /gene="ompN"
                     /locus_tag="ACHLCY_004992"
     CDS             complement(1914..3047)
                     /gene="ompN"
                     /locus_tag="ACHLCY_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP0372050.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGVSNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVDLGLVYQF"
BASE COUNT          884 a          594 c          701 g          895 t
ORIGIN      
        1 ttgatacaaa aagagaagct ttttgttgta agtgataatt aatgatataa atgtagtttt
       61 atgtactttt attttttact aaagcaaaat taagttatta aaatggaggg agaggaatgc
      121 ctgtagattt aacgccttat attttacctg gggttagttt tttgtctgac attcctcaag
      181 aaaccttgtc tgagatacgt aatcagacta ttcgtggaga agctcaaata agactgggtg
      241 agttgatggt gtcaatacga cctatgcagg taaatggata ttttatggga agtcttaacc
      301 aggatggttt atcgaatgat aatatccaga ttggccttca atatatagaa catattgaac
      361 gtacacttaa tcatggtagt ttgacaagcc gtgaagttac agtactgcgt gaaattgaga
      421 tgctcgaaaa tatggatttg ctttctaact accagttaga ggagttgtta gataaaattg
      481 aagtatgtgc atttaatgtg gagcatgcac aattgcaagt gccagagagc ttacgaacat
      541 gccctgttac attatgtgaa ccagaagatg gggtatttat gaggaattca atgaattcaa
      601 atgtttgtat gttgtatgat aaaatggcat taatacatct tgttaaaaca agggcggctc
      661 atcctttgag cagggaatca atcgcagttt caatgattgt aggaagagat aattgtgctt
      721 ttgaccctga cagaggtaac ttcgttttaa aaaattaagg tagcaatggc gggttaactt
      781 ttcgtaaaat gaaattaatt ctcggtacgg catgtaaaac gcggcacctc gtatgcaaga
      841 acgtgccgcg gttggctggt aagcttttcg atagtgcgag tatctaatga tttccagccg
      901 gaactgattc tattggaaag aaatcatggg cgccagattg attagttata gatttttact
      961 gtggcaaaaa tgggacaaaa ggccgcaaaa ggggcaaaaa tggggcaaaa aaagtgtaga
     1021 ttattgtgct ttattgtagt tgttaatcct gcctaacata ttgaaaaaca taaaagttat
     1081 tgttcgtcag atagttgcgg tttttactct gacttgttca ggttgtattg ttctttctta
     1141 ctaatttctt gattttgcga catttaaaag cgactcaatt cgttatatgg catcagaaga
     1201 gtatgcgtca tgccggaacg cccagcataa gaaatctgat ataaaaactg tggcgtgtat
     1261 ggtacggatt agaggggaaa atgtcagcac atttgcgaaa tgaatcaaaa agcccgcagc
     1321 aatgtgcggg cgttagtgtc agcgcacaac cagcacggag cactctgcgt gacgcactac
     1381 agctgcggcg ttagaaccga gcagataagt ggtgatatcc ggtcgatggg aagcaatgat
     1441 gatcatatga gcggggatct tcttcgccaa ttccagaatg cggtcttttg gcgagccttc
     1501 ctcaacatgg acatgcactc tgtcggttgg cagtttaaat tttttaatga tctcttccag
     1561 ttgcgatttg gcttccgctt tcaggtcatc cattgccggt aattctgcgg aatacgctaa
     1621 acccagagag gcatagtagg gcagtgaagg tattaccgtc aggaaatgaa cctctgcatc
     1681 atcaatcttt gcctctgcct caacgtggct aatcacgcgt tgagttaatt ctgaatcgga
     1741 aatatcgata gggacaagaa tcgttctgtt cataaaacct cctgttttag tatccgcata
     1801 aagtgtaacg ccagatgaca ctttttgtgt aatgacggag ttcacatttt taatttagat
     1861 caaaggagga agaataagca gaaaaagccc gccataacag cgggcaggag gatttagaac
     1921 tgataaacca gacctaaatc gacaatatca tcggtagaga tgccgttggc agcgtagaaa
     1981 ctgtcatctt catccaacag gttgatttta tagtcaacat aagtggacat gtttttattg
     2041 aaatagtaag tcgcgccaac atcggcgtat ttaaccagat ctttatcatc aacacctgcc
     2101 gggttgtctg caccaccggc agcgtgcagg tcacggcctt tagacatcag gaaagagact
     2161 gccggacgca gaccaaaatc aaactggtac tgtgcagtga cttcaaagtt ctgggttttg
     2221 tttgcaacag cataatcgct gtcgccaaac ggggtcatat tacgcgtttc tgaatacatg
     2281 gttgccaggt aaatattgtt agcatcgtat tttagcccag cagtccacgc gtctgcttta
     2341 tcaccacccg ccgcagtatg gttaacctgg tcattggtgc ggtcagaaga ggtgtatgcc
     2401 gcaccagcgc taaagcccat gcctaaatca tatgttgtgg aaagacccca gccgtcaccg
     2461 ttttcatggc gaacatcacg tccgttgttg gtgccttcct gaccattact gacaccttcg
     2521 ttgttacctt gatactgcac cgcgaagttc agaccattta ccagaccgaa gaaatcagta
     2581 ttacgataag tcgcgacgcc attggctcga ccagtcataa agttgtctgc attggtataa
     2641 gagtcaccgc caaattcagg cagcatatcg gtccagcctt cgatgtcgta cattacgcca
     2701 taattacgtc cgtaatcgaa agaaccgtaa tctgcaaatt tcagtccggc aaatgccaga
     2761 cgggtccatg actggttttt tgaagattca gtgttgtttg cctgaatatt gtattcccat
     2821 tgaccgtagc cagtaagttg atcgttaatt tgggtttcgc ctttaaaacc cagacgcgca
     2881 tagctctggt cgccatcttt cgctgaatta tcagaaaaat aatgcaggcc atcaactttg
     2941 ccatacagat ctaatttgtt gccgtcttta ttataaactt cggctgcatg tgcagcacct
     3001 gcggcgagca gggcaggaat taaaagtgcc agtactttgc ttttcattga ataaatcctt
     3061 tagttatttt attt
//
LOCUS       DAZGTR010000260         3068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGTR010000260 DAZGTR010000000
VERSION     DAZGTR010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.262"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1680
                     /gene="ydeN"
                     /locus_tag="ACHLCY_004993"
     CDS             <1..1680
                     /gene="ydeN"
                     /locus_tag="ACHLCY_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0372051.1"
                     /translation="KSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAFS
                     DFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIE
                     AAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIP
                     LTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQN
                     RGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFM
                     LYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQY
                     DNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLI
                     SAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEEN
                     IPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDL
                     QQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1732..2889
                     /gene="ydeM"
                     /locus_tag="ACHLCY_004994"
     CDS             1732..2889
                     /gene="ydeM"
                     /locus_tag="ACHLCY_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0372052.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2855..2977)
                     /locus_tag="ACHLCY_004995"
     CDS             complement(2855..2977)
                     /locus_tag="ACHLCY_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372053.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
BASE COUNT          932 a          670 c          623 g          843 t
ORIGIN      
        1 aagtctgcat taaagaaaag tgtcgtaagt acctcgatat ctttgatact ggcatctggt
       61 atggctgcat ttgctgctca tgcggcagat gatgtaaagc tgaaagcaac caaaacaaac
      121 gttgctttct cagactttac gccgactgaa tacagtacca aaggaaagcc aaatattatc
      181 gtactaacca tggatgatct tggttatgga caacttcctt ttgataaggg atcttttgac
      241 ccaaaaacaa tggaaaatcg tgaagttgtc gatacctaca aaatagggat agataaagcc
      301 attgaagctg cacaaaaatc aacgccgacg ctcctttcat taatggatga aggcgtacgg
      361 tttactaacg gctatgtggc acacggtgtt tccggcccct cccgtgccgc aataatgacc
      421 ggtcgagctc ccgcccgctt tggtgtctat tccaataccg atgctcagga tggtattccg
      481 ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac tgcagcagta
      541 ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa acaaacgcgt
      601 gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca aaaccgtggc
      661 tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc cccttcactg
      721 ttcaaaaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt aaccgatgag
      781 gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct ttacctggct
      841 tataatgctc cgcacctgcc aaatgataat cctgcaccgg atcaatatca gaagcaattt
      901 aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt tgatcagggt
      961 gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac aattattctc
     1021 tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg ggcgcaaaaa
     1081 ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat gtggtggaaa
     1141 ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt ctacccgaca
     1201 gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg cgtttccttg
     1261 ctgccctggt tgcaagataa gaaacaaggc gagccacata aaaatctgac ctggataacc
     1321 tcttattctc actggtttga cgaggaaaat attccattct gggataatta ccacaaattt
     1381 gttcgccatc agtcagacga ttacccgcat aaccccaaca ctgaggactt aagccaattc
     1441 tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa caatcagtta
     1501 ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc caatccgcag
     1561 gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca gccaccgctt
     1621 agcgaagtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga agcgaaataa
     1681 ctaaaccttc atgcggcgga attttccgcc gccttattga gcgagatagc gatgcacgtt
     1741 acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt ttaccttgaa
     1801 aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa agagttcatc
     1861 aaacaatata tcgcagcgtc tggcaatcag gtctatttta cctggcaagg cggtgaaccc
     1921 actctggctg gcctggattt tttccgtaaa gttattcact accaacaacg ctatgcaggc
     1981 caaaaacgta tttttaatgc attacaaacg aatggcattt tattgaataa tgaatggtgt
     2041 gcttttctca aagaacatga gtttctggtt ggtatctcga tcgatggccc ccaggagcta
     2101 catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt gatagcagcc
     2161 atcgagcgtc tgaaatcata tcaagttgag tttaatacgt taaccgtcat taataacgtt
     2221 aatgtccatt accctcttga ggtttatcat tttttaaaat ctatcggcag taaacatatg
     2281 caatttatcg aattgctcga aaccgggacg ccgaatattg atttcagtgg tcatagtgag
     2341 aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa gtttatgtca
     2401 accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg tcagtttgaa
     2461 agctttgtca gccgtttttt ggggaatggg cataccagtt gtattttcca ggagtcctgc
     2521 aaggataatc tggttgttga aagtaatgga gacatttacg aatgcgacca ttttgtctat
     2581 ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa cagtgtacaa
     2641 ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata taaacctatc
     2701 tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac tgtttcttat
     2761 ttttgcgaag gttataaaaa ccttttttca accatggtac cttatatgaa cgccatggtg
     2821 gagttagcta agaacagagt accgctttat cacattatgg atgttgcaaa acaaatggag
     2881 aataattaat aacacgccct tttagctcca ttcttagcct ccttcttatg accttaacaa
     2941 ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct tgcgaaaatc
     3001 ctggcttacg tggtatgcgt cagggcatta gcaataccct tgattatcaa tagcataacc
     3061 ataactaa
//
LOCUS       DAZGTR010000261         3007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGTR010000261 DAZGTR010000000
VERSION     DAZGTR010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.263"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            128..1339
                     /gene="bluF"
                     /locus_tag="ACHLCY_004996"
     CDS             128..1339
                     /gene="bluF"
                     /locus_tag="ACHLCY_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP0372054.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1544..2275
                     /gene="bluR"
                     /locus_tag="ACHLCY_004997"
     CDS             1544..2275
                     /gene="bluR"
                     /locus_tag="ACHLCY_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP0372055.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2496..2900
                     /locus_tag="ACHLCY_004998"
     CDS             2496..2900
                     /locus_tag="ACHLCY_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP0372056.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
BASE COUNT          886 a          630 c          646 g          845 t
ORIGIN      
        1 agtgttcaac tttttccatt ttttatcaat gtaaagaaac ggttatgttt tggtattaaa
       61 agaaagacac aagaagatat gtctgttacc gtcttactct cgcctcaccc attaccctgg
      121 attgttaatg cttaccaccc ttatttatcg tagccatata cgtgacgacg aacctgtcaa
      181 aaaaatcgaa gaaatggttt cgatagcaaa tcgcaggaac atgcagtctg acgtaacagg
      241 gatcttactg tttaatggtt ctcatttttt ccagcttctg gaaggtccgg aagaacaggt
      301 taaaatgata tatcgggcta tatgccagga tccacggcac tataatattg ttgagctgct
      361 gtgcgattac gcgcctgctc gccgttttgg caaagcggga atggaattat ttgatttgcg
      421 cctgcacgag cgagatgacg ttttacaggc cgtattcgac aaaggcacat caaaatttca
      481 gctaacttat gatgacagag cgctacaatt ttttcgtact tttgtccttg caaccgaaca
      541 atcaacctat ttcgagatcc ctgccgaaga ctcctggctt tttatcgctg acggatctga
      601 taaagaactt gattcctgtg ccctttcacc aactataaac gaccactttg cctttcatcc
      661 tattgtcgat cccttatcgc ggcggataat cgcttttgaa gccattgtgc aaaaaaatga
      721 agatagccca tcagccatag cggttgggca gcgtaaagac ggggaaatct acacagcgga
      781 tctcaaaagt aaggcgcttg cattcacgat ggcacacgca cttgagctcg gtgataaaat
      841 gatttcaatc aatctattac ctatgaccct ggttaacgaa cctgacgcag tctctttttt
      901 acttaatgaa ataaaggcca atgctctggt gcctgaacaa atcatcgttg aatttactga
      961 aagtgaagtc atatctcggt ttgatgagtt tgccgaagcg attaaatcgc taaaggctgc
     1021 tggtatcagt gtagcaattg atcattttgg cgcaggtttt gctggtttgt tactcctgtc
     1081 acgcttccag cctgacagaa ttaaaatcag tcaggaattg attaccaatg ttcataaaag
     1141 tgggccacgg caggcaatta ttcaggcgat cataaaatgc tgtacatcac ttgaaattca
     1201 agtcagtgct atgggcgtgg caacaccaga agagtggatg tggcttgaat ccgcaggaat
     1261 tgagatgttt cagggagatc tgtttgctaa agctaaattg aatggtatcc cttccatagc
     1321 gtggccagag aaaaaataat tttcagcaca ttctttcaca tgatttcagt aaatcatcgt
     1381 gaaaatgaat acgttacgtt taaatttaat ccacattaaa aataatgttt catccaattt
     1441 agctgaaatc ttatttatac tttgtacaat tttagcgtat attgtacaaa gtattattgg
     1501 gtcgtgtaca ggcgacggag atttgtgacc gcaaggagga attgtggctt attacagcat
     1561 tggtgatgtt gctgaacgtt gcgggattaa tcctgtcact ctccgggcct ggcaacgccg
     1621 ctacggtttg ttaaaaccac agcgcagtga aggcggacac cgactctttg atgaagaaga
     1681 catacaacgc atcgaagaga tcaagcgttg gataagtaat ggcgtccctg taggcaaagt
     1741 taaagcatta ctggaaacca ccagccagga tacggaagat gactggagcc gcctgcaaga
     1801 agagatgatg tcaattcttc gcatggctaa tcctgccaaa ctacgcgcga gaattatttc
     1861 actgggtcga gagtacccag tagatcaatt gattaatcat gtttaccttc ctgttcgcca
     1921 gcgtctcgtg cttgatcaca acacctcccg cattatgagc agtatgtttg acggcgcatt
     1981 aattgaatac gcagcaacct cgcttttcga aatgcgccgt aagcccggta aagaagccat
     2041 tctgatggcg tggaatgttg aagagagagc acgattgtgg ctggaagcat ggcgtttatc
     2101 attgtcagga tggcacattt ctgttcttgc tgatcccatt gaatcgccgc gcccggaact
     2161 gttcccgacg caaacattga ttgtctggac aggcatggca ccaacgagaa ggcaaaacga
     2221 acttttacaa cattggggtg agcaaggtta taaagtcatc tttcatgccc cctaatttca
     2281 cctttatccc gccccttaga cagaatgtta taatctgaac ccacaacagc gtttgtaccg
     2341 gagctggtcc gttgcaaaat gctcaccggg agaattcaga tattccctct atcacactct
     2401 gtacatatca cagccaaagg acagaccaga ttgaagtggt aaaaccgatc gtgcacacta
     2461 cgcagtctca caactaacaa gaggttatta tcctgatgga tcaggtcgtt gtttttcaaa
     2521 agatgtttga gcaagtgaga aaagagcaaa acttctcctg gttttattca gaattaaaac
     2581 atcaccgtat tgcacattac atttattatc tggctacgga taacatcaga attattactc
     2641 acgatgacac ggttttgtta ttaagaggaa ccaggaacct gttaaaagtt agtacgacca
     2701 agaaccctgc taaaataaaa gaggccgcat tgcttcatat ttgcggaaaa tctacatttc
     2761 gggaatactg ttcaacacta gcaggcgcgg gcgttttccg gtgggttact gatgttaatc
     2821 ataacaaacg cagttactac gccattgata atacgctttt atatattgaa gatgtagaaa
     2881 ataataaacc attaatctag ctaaagttgg atgcttaaga aatgcttcat aattcagtaa
     2941 ggcattagca taatgggaat aaaagtacag agactatccc tatggatgat aaatactgtc
     3001 cttttat
//
LOCUS       DAZGTR010000262         2995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGTR010000262 DAZGTR010000000
VERSION     DAZGTR010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.264"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(7..576)
                     /gene="ybfC"
                     /locus_tag="ACHLCY_004999"
     CDS             complement(7..576)
                     /gene="ybfC"
                     /locus_tag="ACHLCY_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP0372057.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIAEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
     gene            complement(573..2093)
                     /locus_tag="ACHLCY_005000"
                     /pseudo
     CDS             complement(573..2093)
                     /locus_tag="ACHLCY_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(2124..2450)
                     /gene="ybfB"
                     /locus_tag="ACHLCY_005001"
     CDS             complement(2124..2450)
                     /gene="ybfB"
                     /locus_tag="ACHLCY_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfB"
                     /protein_id="HFP0372058.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(2450..>2995)
                     /locus_tag="ACHLCY_005002"
     CDS             complement(2450..>2995)
                     /locus_tag="ACHLCY_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0372059.1"
                     /translation="YITQDPIGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMG
                     GTGERSGPDIWGNPFYHQYLCVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEA
                     AGRCDLVETDNSCVENCLKGKFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETC
                     KMKCSGNNIGRFIRFVFTGVM"
BASE COUNT          739 a          748 c          631 g          877 t
ORIGIN      
        1 gagattttat ttctcttcac tacgaattag ttcatgctgt atctttatta aacattcgta
       61 agactcattt ttgttcggga gatgtgctgt cattcgagca taagttggtt tctcatcttt
      121 aaaatatttt accatgattg aattatcaag gactgccaac aactgaactg gatcactgct
      181 ctttttttga tagatacccg ctcttatata acttctaaac cgatcgccga gaaacgccgt
      241 tttttctgct gtatttatca actcctgcaa cgttcctttg atggcatgat tttcattaag
      301 gcgtagtgac ccgacgcgaa ttaagagtct cgccccgctt ggtgtgaatt tattattttt
      361 acatgtaccc agttctgttt gccaataata ctcagcgatg tcctcattct gataccatct
      421 ccacatggct ttattattta agacaggaaa cgcggaaaaa atagtaccct tagcgaaaat
      481 agtaaaacaa tcatgatctt tagcttgtat ttcgcaatca gctataacac caaaactaac
      541 aaatatcatc agcaaaaaga acaaaactct tttcattttc ttattcctct cgatgttctc
      601 tgtttcatct tagctaaaat ttcacccaaa tcatctatcc ctgctgttgc tggcccatcg
      661 ggtttattaa tgtctgggaa taaatagggt tctgcacaag tatatcctgg tggtaattga
      721 gttgttggcc aatttgtctg tggcaggtaa tcacttgctg tctttgtatc catccctgga
      781 tactgggaat catcctgtgg cgtacaagtc agatttggtt ttacccactt cctgcaatac
      841 agtgtgtagt cgtaagggct taatgctctg aagaatgttt tgaaaacatt aggagcacta
      901 ctggcctggt caataggata tggcagtctt aattgtttcg ctatgtccgg caatttactt
      961 ggatcgggcg taggcggttt gttgggtctc gctccatatc cgccattatt taagtgtcca
     1021 tattttgatg ccagtcccat tgaatctata tactgaaccg gattcagcgg atactgataa
     1081 aaattccatc cccccttcag cccaatcgga tcctgagtga tatacctccc ctgcagcggg
     1141 tcataatagc ggtggcggtt gtaatacagg ccggactcct catcatactg ctgccccggc
     1201 aggcggataa gctgctgcag ctgatgcggg ttctcttcat tcagcaggtt gccccattca
     1261 tcgtattctg cgcaccattc tgttgcccct tccgtgctga caagcgccag cggcaggccg
     1321 cgatggtcgc agtggtacag gtggattttt cgcgccggcg tgtacaccgg gtccatctgg
     1381 ttttgcatct gctccaccgt caggccgcac gatgccagcc agcggcggct ttcctcactc
     1441 acccggtcag ccaggatttc actttccagc cggtcgagca tctgcaccag caccggcggg
     1501 aacaccacac tgccaccgtc ttcgccgccg gactgctgaa gggtatccgc caggctgcgg
     1561 cgctgcgttt tcgcctgctc accggtggcg gtttcaaccc tgatgagtgg cgtgaagctc
     1621 cccggctgat aaatcgtctg gatgcgggtt ctgtcgttct gtattgtggt caggcggtcg
     1681 ccgtcccagc cgtaccaggt cacttgcggt ttccgtgaca gcgacatcca gcccgtcagg
     1741 tcccgttcac gtcgccacac ccgttttgcc accctgcggc ccagcgggtc gtaaagatag
     1801 cggctttcga ccagcggctc tgcatattgt gtccgcgtgt agtgcaccag ccggtgctga
     1861 ctgtcgtaat ggtaccggtg ggtgcgctca tcatccgtgc ggataacccc ttccgggatg
     1921 aggtcggttt tctccgtcag cctgccgtga cggtcatacc ggtaaagata gtgcgcgtca
     1981 cgggcgatac ggttatccgg ccacatgctg agggcgctgt ccgggtacag ctccgggtcg
     2041 ggcaggcggt tacctgccgg gtctgtggca tacgggatat caatgcccag gttacatata
     2101 ataataagag caattaactt aatttatttg tttataatgc gcttacttaa tacaaaccat
     2161 tgaatatacc ctcctgacag aaaataaagc catattatta tcgccataat atacttacta
     2221 tcaatcctta cgccaataat gtctgataca gtccctataa atattagagc aataaagaat
     2281 actatgcctg ttggaaaaca tattaccatc attccataag acatgagact aattaactcg
     2341 ctcacatcac gagtggagtc taatagtgaa agtctatgca tggaaaagaa tagtattaaa
     2401 agcgataaag ccagccatat agctctgaat aaaaaaataa tatatttcat tacatcactc
     2461 cggtgaatac aaatctaata aaacgtccaa tgttatttcc ggagcacttc atcttacatg
     2521 tttctaaaga atcattagac cagtcttggc agtttttaaa tagccctaaa tttataggtg
     2581 tgaatatatc aggcaataca gaataacgcg gccttacctc tttaaacttc ccttttaaac
     2641 agttctccac acaactatta tcggtttcaa cgaggtcaca acggccagca gcttcttttg
     2701 tatcattact tgctttacct ggcccccata gaccatctcc ttttgattct ccccgttggt
     2761 cttggccacc acaagtatag tcccctttac catctgggac acaaagatat tgatgataga
     2821 acggattccc ccatatatct ggaccgcttc tttcaccagt tccgcccatt gaatgcagtg
     2881 gcttaatgca ttttagtgtt tctaatccta atggatctat atttgagatc ggattcaacg
     2941 gatactgata aaaattccat ccccccttca gcccaatcgg atcctgagtg atata
//
LOCUS       DAZGTR010000263         2967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGTR010000263 DAZGTR010000000
VERSION     DAZGTR010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.265"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..783
                     /locus_tag="ACHLCY_005003"
     CDS             <1..783
                     /locus_tag="ACHLCY_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004030576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP0372060.1"
                     /translation="KEDNGNISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHM
                     SRRVTTDKDTDKDTDQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALK
                     ADSGAVQQVMTARPEQSHQLQQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAILDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            790..1536
                     /locus_tag="ACHLCY_005004"
     CDS             790..1536
                     /locus_tag="ACHLCY_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0372061.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLVEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            1558..2274
                     /locus_tag="ACHLCY_005005"
     CDS             1558..2274
                     /locus_tag="ACHLCY_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0372062.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            2307..2588
                     /locus_tag="ACHLCY_005006"
     CDS             2307..2588
                     /locus_tag="ACHLCY_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0372063.1"
                     /translation="MILRQCAGTMKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2585..2812
                     /locus_tag="ACHLCY_005007"
     CDS             2585..2812
                     /locus_tag="ACHLCY_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372064.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2805..>2967
                     /locus_tag="ACHLCY_005008"
     CDS             2805..>2967
                     /locus_tag="ACHLCY_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372065.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRD"
BASE COUNT          804 a          649 c          919 g          595 t
ORIGIN      
        1 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc ctgcagagcg caagagggcg
       61 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcgc gtcacgaaat
      121 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
      181 gaagatcaaa acactatggt ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt
      241 cagaccgtca atcctggtca gccagcaggc acgacaccgg aagccgattc agcgtatgcg
      301 ctgaaagccg attcgggcgc tgtgcagcag gtgatgaccg caaggccgga gcaatcacac
      361 caactgcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
      421 gaaaacaccg ggcagcctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
      481 cgggaatacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
      541 gccagattac gcgagggggt gccaccagag gccatactgg atggtgtgag gcgttacgca
      601 agatacctgg cggctaccgg gaaaacggga acggaatttg ttcagcgagc gacgacgttt
      661 tttgggccgg accggaattt tgaaaacccc tggttgctcc cggtaagcgg cacgaacaac
      721 cagcgttgtg tgaatcatat ttctgaaccg gataccgaaa ttccgccggg attcaggggg
      781 tgatgtggca tgaaaaacat tgcggcagcc ggggttcttg aacgtattcg cagacttgca
      841 ccacaggcgt cggttccacc gtaccggacg gtggaggagt ggcgggaatg gcaacttgtt
      901 gaaggacgaa aacgcagcga ggagattaac cgccagaatc accagttgcg ggtggaaaaa
      961 atcctgaatc gttcgggcat ccagcctctg cacagcaaat gctcgtttgc gaattatcag
     1021 gtgcagaacg acgggcaaaa atacgcgctg agccaggcca aatccatagc tgacgaactg
     1081 atgaccgggt gcacgaattt tgtgttcagc ggtaaaaccg gcaccgggaa aaatcacctt
     1141 gcagcggcga tgggtaaccg gctgatggcg aaggggcgca gcgtgattat cgtcaccgtg
     1201 tctgatgtca tgagcgtgtt gcatgacagc tacgacaacg gcaaatccgg ggaaaaattt
     1261 ttacaggagc tttgcagtgt tgatttgctg gtcctggatg aaataggcgt tcagcgggag
     1321 acgaaaaacg agcaggtggt attacaccag ataattgatc gccggacagc atcactgtgc
     1381 agtgtcggga tgttaacaaa cctgaatcat gccgcaatga gcacgcttct tggtgagagg
     1441 attatggacc gcatgaccat gaacggtggt cggtgggtga cgtttaactg ggatagctgg
     1501 cgtccaaatg tcagcaatca gagggttgtg aagtaatttt tgttggagga cgttttaatg
     1561 gaaactgtat ttgacgcact gaaagcactg aaaaaagcct cttcacaggt agtggcatcg
     1621 cgccttggaa tcagccgcga agatgctgtc aacgaactgt ggaaactgaa gcgccgtggt
     1681 gaagcggata acaagggttc gatgtggtgg ctgattcagg ctggtgaaag tgaaccagtg
     1741 tcaccggtac cgaaagtgac agcgcaaatg ctgactgagg cgattgaaca tcatggccca
     1801 caaacggcgg atgagctggc cctgatgttc gggattacct cccgccgggc gaattcatca
     1861 ctggccatgg caatcagcaa agggcgtctg attcgcgtga atcagggcgg taaatttcgg
     1921 tactgcatac cgggcgctga tttaccggca gagccgaaag ccgcatccat agcggaaacg
     1981 gatggtaaag cctttcctca gccagcaggt gttgcgttac cagtcgggga agcggaaaca
     2041 caggaagaaa taaaaacgga aagtgtggcg gtcacagtgc agtcacagcc gtcgttcacc
     2101 agaaagcatc cggatggtct gattttacca tcgctgcatg tggctaaccg cgagctgcgc
     2161 cgggcaaaag gtcaggttca gaagtgggag cgagtctgcg ccgcgctgcg ggagctgaac
     2221 aagtgccggg atattctccg ggatattacc gccaccagag aacagcagcg gtgagtgggt
     2281 ggaagacgtg gtgccgggcg gaaatcatga tactccggca gtgtgcggga acgatgaagg
     2341 taaaaagcgt tggcgcactt atcggacgaa ctgaagcggc agtgagaacg aaggcacggg
     2401 agctgggcat cagcatgatg ttacgtggtg attttcaccc gtcggcaaaa tattctcagc
     2461 gtgatattga gctggcgcgg caactgcatc agagaggcat gcaaagaagg gaaattgcca
     2521 gaaaattagg catgccgctg cgcatagtga ataactacgt ttatttcgac aggagggtgt
     2581 ctgcgtgaaa atcctgtatc aggattacgg cccggtgggg caggtggtta tcagcagtac
     2641 tgtaatggag tttcggaagc ataaccgtgt ggtggatgct gtgctgttaa cctgtccggg
     2701 gatatcggcg agtcgtgcag gtgtgtttat tatgaagacg aaattatatg gcagtaaggc
     2761 gtggataaag aaggcgtatc gtgtagcgtt gcaggaggtt aacagtgagt aaaattaaag
     2821 aaatgccggt agttcgtgac ggatatggct actggacaca tcctgaatat gaaaaattct
     2881 gtgatggtcg ggaatatatt tcaacggaag agtttaacgc ctggatggag gaaaataatc
     2941 ttcaatacgt cctctgcttc agagatg
//
LOCUS       DAZGTR010000264         2956 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGTR010000264 DAZGTR010000000
VERSION     DAZGTR010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.266"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..776
                     /gene="cif"
                     /locus_tag="ACHLCY_005009"
     CDS             3..776
                     /gene="cif"
                     /locus_tag="ACHLCY_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFP0372066.1"
                     /translation="MSVNTEAVLSPMQHTSALHVRDFASLCSQNLKANVLLNSDDHEV
                     PIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREKGIVEPSCGVTANAIMKLF
                     LDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVNDGALGHAYIVDIPKGENSC
                     RPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNYFSCMSQNKTDLEQIATLF
                     DIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIEKTLR"
     gene            complement(912..1432)
                     /locus_tag="ACHLCY_005010"
                     /pseudo
     CDS             complement(912..1432)
                     /locus_tag="ACHLCY_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1476..1556
                     /locus_tag="ACHLCY_005011"
                     /pseudo
     CDS             1476..1556
                     /locus_tag="ACHLCY_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            1715..2289
                     /locus_tag="ACHLCY_005012"
                     /pseudo
     CDS             1715..2289
                     /locus_tag="ACHLCY_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
     gene            2490..2870
                     /locus_tag="ACHLCY_005013"
     CDS             2490..2870
                     /locus_tag="ACHLCY_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372067.1"
                     /translation="MNEIRSNPVKFLEEHLILNTQRYAHHDETSLITVNITREDGLLR
                     LKETEYDMAGSDYILFTAMRDTDSPERFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQ"
BASE COUNT          887 a          530 c          649 g          890 t
ORIGIN      
        1 ccatatctgt aaatactgaa gctgtattat ctcccatgca acacacttca gccttacatg
       61 taagagattt tgcttccctg tgctcacaga acctcaaagc taatgtattg ctaaatagtg
      121 atgaccacga agtacctata catcagaaaa atcctgctgc aataatgcaa aatatcgact
      181 ctaacatcaa acagatggca acagactggg ggatgtcgat tgaggaggtt gaggttatta
      241 tagggcgaga gaaaggcatt gtggaaccct cctgcggagt taccgctaat gctattatga
      301 aactatttct ggacaaggat ggcttcagtt actgctttga aaatgaacag acactatcgc
      361 tcgagcagct tcaggagcgc ctgtcctgta tgcctgaatg taagagcttt gtattacgtg
      421 ttaatgatgg tgcgcttggt catgcttaca ttgtcgatat tcccaaagga gaaaactctt
      481 gtcgtcctgc attcttgtat cagtcagatt taggagaggg cgtcaccaga aagttaagat
      541 ttgaggactg gatgacgcat aaagcattga ctccgatttt gctggatgat atttgtaatt
      601 acttctcctg catgtctcaa aataagacag atttggagca gattgcaacg ttatttgata
      661 ttgatggaaa tgttaaaatg ttacgaaagg aaaatattca atatcaaaag catgacaatt
      721 ttagtttcca gttgtttgag tatgacaccg ataatattga aaaaacattg agataataaa
      781 atcactatgt agttagcttt tgttaaaact gcaattattt gtaaacataa taataagtat
      841 tatgacgtta aaatttaacc atacggtaaa atgattcagg aaaaccggtt cagcatagcc
      901 taggctgaac ttttgaggat atcaacgaac ggaatttttg aacatttatg agtagatcgt
      961 tgttgaaggg cttcaatgag catgtcaagt tcatcaactg ctggtctacg attacgacgg
     1021 tttgatttac caatcaaagc aagtttaagt agatatggac gagcactttt cgccgggttt
     1081 gatgtgtaat taattccgta tataggtttg gctgcatcca gaacactgcc aagataacta
     1141 acatcgtgac tgactgttgc tggacctgca ccagcgttgt ttctcagcct gcaatgttca
     1201 attacgtcat tttctgtcag ttcagatagt ttgatcgcgg agatgtcact atccataagc
     1261 agttctagca catatctttt agtacggtct gctttacctc cggcatttgg tcatttaaat
     1321 atttgtgtag taagtcacgg actgtaagtc cgtcaacagc atttgatgat gaaatgccat
     1381 atagatctaa taccatcact ttctgtgtgc cccatgtttt agcatgagca tgaacgcggc
     1441 ggctatctca cgggtgatca tccctttggc atactgaaaa gtatcgggtg ttactgaacc
     1501 gtgttgatac atcaactgca ctggatattg agtggccgat aataccgacg ttataaccta
     1561 taaatgccaa tcaggctgaa tgttgaaagg atagaatgaa cagcccgaca cacaaaaaac
     1621 ggagtccggc tccggttttt gtgttgccat ataaggcaga tgtttgttac agctatttaa
     1681 gtctggagtt caaattaaaa tagggagttt tgttatgcca ttaacctcag atattggatc
     1741 acgttcattt aatcttgggc tggaggtttt tcgtgcccga attgcagcca atgggcgcgg
     1801 agatatacag taggtggtga aactgtcagt attgtgtatg atgctactaa tgggcgcttt
     1861 tcatccagtg gcggtaatga cggattgctt tctgagttat tgattttggg atttaataat
     1921 ggtcctcgag cccttagtga gagaatgcta agtatgcttt cggactcagg tgaagcacaa
     1981 tcgcaagaga gtattcagga caaaatatct caatgtaagt ttcctgttag ttcaggaaat
     2041 ttccagtgcc cgccagagtc tattcagtgt ccaattacac tagagagacc cgaagaagga
     2101 gtgtttgtca aaaattcaga tagttcagca gtatgcactt tatttgatgt tgatgcactt
     2161 tctcgcgtgg ttaatgacgg ttcagttcac cctctgacac gagctccaat aaccccatca
     2221 atgattgtta aaccagaaga gtgtaaatat gaccctgcaa ggggaagctt tattataaaa
     2281 gatagttaag atgtttcaaa tgaaacaata ttaacttccg ataatttata taaaaacacc
     2341 acaggcattc ggggcctgtg gttggtaaaa caatataata cgcgagttat ttttcatgag
     2401 ctggagagaa aacaatcaag gtaagtagta ttatttcaca aaataccggg cactttctgg
     2461 tgccgcatcg ttcagagctt cggtgtataa tgaatgaaat acgtagtaac cctgtaaaat
     2521 ttctggaaga gcatttaatt cttaatacac agcgctatgc ccatcatgat gagacatctt
     2581 taattacagt taatattaca agagaggatg gcttgttaag actaaaagaa acggaatatg
     2641 atatggctgg tagtgattat attctgttta cagctatgcg tgatactgat agtccggagc
     2701 gctttgatgc acctgcagtt gtaagcttga gtaatcagtg tataagcctc cggcgaaatg
     2761 atatttctca aactatccag caaagttcac cgttatggct aactaatcag caaagcggct
     2821 gtagtgttct tattgttcgc catggattat cagaatctgg agaacagtag tattcaatgg
     2881 tacatatgtg cccaggggat agtaacgatt tcgtcgatga gtttacgcta ataatcagga
     2941 gattttgttg gagatg
//
LOCUS       DAZGTR010000265         2925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGTR010000265 DAZGTR010000000
VERSION     DAZGTR010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.267"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            89..165
                     /locus_tag="ACHLCY_005014"
     tRNA            89..165
                     /locus_tag="ACHLCY_005014"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:123..125,aa:Arg,seq:tct)
     gene            256..1215
                     /gene="stxA2"
                     /locus_tag="ACHLCY_005015"
     CDS             256..1215
                     /gene="stxA2"
                     /locus_tag="ACHLCY_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A, a/c/d subtype"
                     /protein_id="HFP0372068.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRTVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1227..1496
                     /locus_tag="ACHLCY_005016"
     CDS             1227..1496
                     /locus_tag="ACHLCY_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFP0372069.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
     gene            1982..>2925
                     /locus_tag="ACHLCY_005017"
     CDS             1982..>2925
                     /locus_tag="ACHLCY_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0372070.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGS"
BASE COUNT          732 a          653 c          783 g          757 t
ORIGIN      
        1 gccattagct catcgggaca gagcgccagc cttcgaagct ggctgcgcgg ggttcgagtc
       61 ctcgatggcg gtccattatc tgcattatgc gttgttagct cagccggaca gagcaattgc
      121 cttctgagca atcggtcact ggttcgaatc cagtacaacg cgccatattt atttaccagg
      181 ctcgcttttg cgggcctttt ttatatctgc gccgggtctg gtgctgatta cttcagccaa
      241 aaggaacacc tgtatatgaa gtgtatatta tttaaatggg tactgtgcct gttactgggt
      301 ttttcttcgg tatcctattc ccgggagttt acgatagact tttcgaccca acaaagttat
      361 gtctcttcgt taaatagtat acggacagag atatcgaccc ctcttgaaca tatatctcag
      421 gggaccacat cggtgtctgt tattaaccac accccaccgg gcagttattt tgctgtggat
      481 atacgagggc ttgatgtcta tcaggcgcgt tttgaccatc ttcgtctgat tattgagcaa
      541 aataatttat atgtggccgg gttcgttaat acggcaacaa atactttcta ccgtttttca
      601 gattttacac atatatcagt gcccggtgtg acaacggttt ccatgacaac ggacagcagt
      661 tataccactc tgcaacgtgt cgcagcgctg gaacgttccg gaatgcaaat cagtcgtcac
      721 tcactggttt catcatatct ggcgttaatg gagttcagtg gtaatacaat gaccagagat
      781 gcatccagag cagttctgcg ttttgtcact gtcacagcag aagccttacg cttcaggcag
      841 atacagagag aatttcgtca ggcactgtct gaaactgctc ctgtgtatac gatgacgccg
      901 ggagacgtgg acctcactct gaactggggg cgaatcagca atgtgcttcc ggagtatcgg
      961 ggagaggatg gtgtcagagt ggggagaata tcctttaata atatatcagc gatactgggg
     1021 actgtggccg ttatactgaa ttgccatcat cagggggcgc gttctgttcg caccgtgaat
     1081 gaagagagtc aaccagaatg tcagataact ggcgacaggc ccgttataaa aataaacaat
     1141 acattatggg aaagtaatac agctgcagcg tttctgaaca gaaagtcaca gtttttatat
     1201 acaacgggta aataaaggag ttaagcatga agaagatgtt tatggcggtt ttatttgcat
     1261 tagcttctgt taatgcaatg gcggcggatt gtgctaaagg taaaattgag ttttccaagt
     1321 ataatgagga tgacacattt acagtgaagg ttgacgggaa agaatactgg accagtcgct
     1381 ggaatctgca accgttactg caaagtgctc agttgacagg aatgactgtc acaatcaaat
     1441 ccagtacctg tgaatcaggc tccggatttg ctgaagtgca gtttaataat gactgaggca
     1501 taacctgatt cgtggtatgt gggtaacaag tgtaatctgt gtcacaattc agtcagttga
     1561 cagttgcctg tcagactgag catttgttaa aaaaatttcg catggtgaat ccccctgtgt
     1621 ggaggggcga ctggtgaaaa atccttgctt gtgattcatt atcgacacgg gttcggtggt
     1681 accaggccga actcaccggg aggcacccgg caccatgcag tatacagaga ttaggcatat
     1741 accaaggcct ctcatagcag gggccttttt acatgtaaaa aagcccgagt gggttcgggc
     1801 aattgcatga gatactcgtt ttaataatcg aaagcatttt aaccaggatt cataaggctg
     1861 cgcaactgcg cggccttttt cgtatttcgg gctgtagtct tccttctgcc attgtcctgt
     1921 aacttccgga cttcagcccg ctcctcatct gactcacaca ttatcccgac cgggaggatt
     1981 catggcattt aaacactatg acgtggtcag ggcggcgtcg ccgtcagacc ttgcggaacg
     2041 aataactcaa aaactgaagg aagggtggca gccttatggt agtgcgctga tttcgacagc
     2101 tggttatggt gcggagttca tccagccagt tgtgagtgag gggagcatct catcaccaga
     2161 ggagccaggc aaccgtccga cgacctcagc gccttctgtt gcgccagaat attactatgt
     2221 gatcgcgctt gctggtcagt ccaatggtat gtcatacggt gagggactgc cattaccgga
     2281 tacattcgac agtcctgatc cacgaattaa acagttagcg cgtcgcagta cggtgacacc
     2341 gggaggtgcc gcctgtaagt ataacgacat cattccggcg gaccattgtc tgcatgatgt
     2401 gcaggacatg agccgcctta accatccgaa agcggacctg tcaaaggggc agtacggaac
     2461 cgtggggcaa gggctgcata tcgccaaaaa attgctgccg tttataccgg cgaatgcggg
     2521 cattctgctg gttccgtgct gtcgtggtgg ttcagcgttc accaccggag ccgatggcac
     2581 atacagtgac gcgagtggtg cctcggagaa ttcaacccgc tggggtgtgg acaagccgct
     2641 gtataaggac cttatcggtc gaacaaaagc agcactgaag aagaatccga aaaatgtgct
     2701 gtttgccgtg gtgtggatgc agggggaatt tgattttggc ggtacgccgg taaatcacgc
     2761 agcacagttt ggtgcgctgg ttgataaatt ccgtgcagac ctggcggata tggcaggtca
     2821 gtgcgtcggt ggctctgctg gcggtgttcc ctggatatgc ggggacacga cgtatttctg
     2881 gaagcagaag aacgaatcca cgtaccagac ggtgtacggc agcta
//
LOCUS       DAZGTR010000266         2879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGTR010000266 DAZGTR010000000
VERSION     DAZGTR010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.268"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..713
                     /locus_tag="ACHLCY_005018"
     CDS             <1..713
                     /locus_tag="ACHLCY_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFP0372071.1"
                     /translation="MTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKFKKLGM
                     IDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTSKPAIPMPVSNTVPADYQQITTDK
                     EYNNIISNTDVLESATADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDKRKNQI
                     RTFWRKAGVITRQLDGHGFTMQDWRNYLSYVGENCRWMFEERPNHQRGTIWHKKGFDF
                     LLNDNTYLKVREGEHDDR"
     gene            703..2139
                     /locus_tag="ACHLCY_005019"
     CDS             703..2139
                     /locus_tag="ACHLCY_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372072.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKILFEEITRMHREQKPVDGLTLFDELERKSLTVSVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDMLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            2139..2408
                     /locus_tag="ACHLCY_005020"
     CDS             2139..2408
                     /locus_tag="ACHLCY_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372073.1"
                     /translation="MNKKQLAILEKAWDAQISYALKEQALPIIQTKSKIARQLCDGGF
                     LNEVEITRQMVTFKGYEINHHGIAAYCSHLPDDVDIDEMEREMKQ"
     gene            2478..2756
                     /locus_tag="ACHLCY_005021"
     CDS             2478..2756
                     /locus_tag="ACHLCY_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372074.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDLWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
BASE COUNT          878 a          625 c          739 g          637 t
ORIGIN      
        1 taatgactga cgcaggagaa attctggtag gcagagggaa gctacttggc ggtagaaact
       61 ctctggcgtt tgaaacagga ctcaaaccag atcgcgttca gtacctgctt agaaagttca
      121 aaaaactcgg catgattgac tgggtttcac acggtaaatt ctcagttttc tcggtagaga
      181 aatatgacga ttatcagtca aattttgtac cagcagatta ccagcaaatt accacctcaa
      241 agccagcaat accaatgcct gtaagcaata ctgtaccagc agattaccag caaattacca
      301 cagataaaga atataataat attatctcta atactgacgt attagagagt gccacagcag
      361 acaaaaagtc tgacaagaaa aaaccttccg tcagctgtca ggatgttgtc gatgcttacc
      421 acgaaatcct tcctgaagcg ccaagaatcc gcgcactgaa tgacaagcgt aaaaaccaga
      481 tccgaacgtt ctggcgcaaa gccggagtga taacacgcca gcttgacggg catgggttca
      541 cgatgcagga ctggagaaat tatttgagct acgtaggcga aaattgccga tggatgttcg
      601 aagagcgtcc aaaccatcaa cgcggaacta tctggcacaa aaagggattt gatttcctgc
      661 ttaacgataa tacctacctg aaagttcgtg agggtgaaca cgatgaccga taatttttat
      721 gcgccgcccc atagcatcga ggcagagcag gcggtgattg gtggattgct tctggatgat
      781 gacagcagtg agcgcgtcca gaaagttctg gcgatgctga agcctgattc attttacagc
      841 cgaccacaca aaatcctttt cgaagaaata accagaatgc accgggagca aaagccagta
      901 gatggcctga cgcttttcga tgaactggag cgtaaatcgt taacggtgtc tgttggcggt
      961 tttgcttata tcgctgagat cgcaaagaac acgccaagcg cagcaaacat cgttgcctat
     1021 gcaatgcagg ttcgtgaaac cgcaatggaa cgctacgcca tcaaccgcat gactgaagcg
     1081 acggaattgc tctattcccg caacggaatg actgcgacgc agaagtacga agctattcag
     1141 gcgattttca cgcaactgac agaccatgca aaaaccggat cgcgtcgcgg ccttcgctca
     1201 tttggcgagg tcatggaaga ctgggttagc gaccttgaga agcgatttga cccatcaggc
     1261 gaacaacggg gaatgagcac agggatccca tcgctggaca ggatgctgtc accgaaaggt
     1321 ctggtgaaag gctctctgtt tgtcattggc gctcgcccta agatggggaa aacgacgcta
     1381 tacagccaga tggcaatcaa ttgcgcagtg catgagaaaa agcccgctct gatgttcagc
     1441 cttgaaatgc caggtgatca gatactggaa aaactggtag ggcagaagtc tggtgttaac
     1501 ccgaatattt tttaccttcc ggcgacaaat gacgccgatg acggctatca gggtgattac
     1561 gatggtgact tcaacagggc gatcgaaaca gccaatcgct tgagtgaaat cgacatgctt
     1621 tacatcgacg acacgccggg attatctctg gctcaaatcg tcagcgaaag ccgtcgaatc
     1681 aagcgagaaa aaggatgtgt tggcatgatt ctggtcgatt acctgacact aatgaccgct
     1741 gagaaggccg atcgcaacga ccttgcttac ggcatgatca ccaaaggact gaagaacctt
     1801 gccaaagagc ttgattgcgt tgttgtgctt ctgacgcaac ttaaccgcgc actggaaagc
     1861 cgaaccaata aacgcccatt accaagtgac tcacgagata cagggcagat tgaacaggat
     1921 tgcgattatt gggtggggat ccatcgtgaa ggtgcttttg atgacagtgt tccacctggt
     1981 gaaaccgaac taatccttcg tctcaatcgt catggcaata ccggcacggt gtattgcatt
     2041 caggcaaatg gcgctattta tgacacagac caacagtctg ctgaaatgcg ccgacgtgaa
     2101 cgcgaggaac cgcagtccaa gaagaaagga ggattctgat gaataaaaaa caattagcca
     2161 ttctcgaaaa ggcatgggat gcgcaaatat catacgcttt gaaagaacag gcactaccaa
     2221 taatccagac caaatcgaaa atagccaggc agttatgcga tggcggattc ctgaacgaag
     2281 ttgagattac gcgccagatg gtaacgttca aagggtatga gataaatcat catggtatag
     2341 cggcgtattg ctcccatctt cctgatgacg ttgacattga tgaaatggaa agggagatga
     2401 agcaatgacc atctacatca ctgagttaat aacaggcctg ctgtaatcgc aggccttttt
     2461 atttggggga gagggaagtg aacgatagct accgacagtt tgaaaactgg tggtcaaaag
     2521 acaaaagcca gttcacggga gacgatgaat taaaagagtt tgcctgggtg atatggcagg
     2581 catcgcgctc tgctattgaa ctggatatcg actggcccga atcgaatgac gacctttgga
     2641 aagatggtga agaaggtgct tatgcgatgg gttatgagga tgggcgtgac aaaacggtaa
     2701 ttgcagtaat gaaagccatc agggccgcag gaatcaaaga aaagaatttc gattaagcaa
     2761 atatcatttc aataaatcgc ttttaaggca tcacaatcgc tctgtggtga ggtaagcacg
     2821 tgcaaggtat accgataagc agcgagaatg aaaaatgcgt cagaatgcgt ttgaggagg
//
LOCUS       DAZGTR010000267         2855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGTR010000267 DAZGTR010000000
VERSION     DAZGTR010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.269"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(158..540)
                     /locus_tag="ACHLCY_005022"
                     /pseudo
     CDS             complement(158..540)
                     /locus_tag="ACHLCY_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830158.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(619..1536)
                     /locus_tag="ACHLCY_005023"
     CDS             complement(619..1536)
                     /locus_tag="ACHLCY_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFP0372075.1"
                     /translation="MDKTSPYYRQVALLVRALPWVANESCFALKGGTAINLFIHDFPR
                     LSVDIDLAWIPLESREVALPNVREALTRIAAELEAQTGIRAVLQTHNPDEMRIIVTTD
                     DAQIKIEVSPVARGTLYPPQERDIVGAVEEEFGFAALPVVSLPDLYGGKLCAALDRQH
                     PRDFYDVKMLLDTQGIDRSIFNGFIVYLLSHNRPLSEVLNPRWKDITEPFQNEFSGMT
                     FEEVSLEELKEVPERMLSTLKTHFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQW
                     KLRNIGQMSEEKRAQSLEKLEKVMTEWLR"
     gene            complement(1529..2302)
                     /locus_tag="ACHLCY_005024"
     CDS             complement(1529..2302)
                     /locus_tag="ACHLCY_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HFP0372076.1"
                     /translation="MASKLNWLLQNTSPGSLVLQSWLTRNNISPSLAFKYAQNGWLIK
                     LRAGVYARAGREPDWSDALWCLQNQLEAPVHLAGLSSLSWQGRSHYLQLKQNQCWLSV
                     ENKAILPKWFKEFPAVEWLMVSALKLPDLDEKYRTELEVKGKRITASTPELAAYELLS
                     AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWFKRID
                     TSKINLGSGNRQIVVNGKLNTQYQITVPENFTKKGISHG"
     gene            2386..2706
                     /locus_tag="ACHLCY_005025"
                     /pseudo
     CDS             2386..2706
                     /locus_tag="ACHLCY_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          738 a          659 c          669 g          789 t
ORIGIN      
        1 ttgttcctgt tctttattgc ttttgcgagg ttgattcccc gacctgaaga atgtaattta
       61 ttaaataatc tactacagac ccagtataat aattcatccg aatgattcat tgatgagggg
      121 gcgaacataa atactttttg gaagccctac tcgacaacta tatcgccatc ccaatcagaa
      181 tagttcctgg tatcaaaaca tactctggta catgaaaaat cgacatcgcc tgtaacagaa
      241 tcgccaggcg cagcagaaag gtgagggtaa cattgcccac aggcagcaac gcacggtgga
      301 ttacggtggt gaagcctccg tccaaaacaa tttattctga ctattggatg aggaaatcag
      361 catcgtgatc ccttgctaca atttactgct gataagcgcc agatgttcta ttaaccacat
      421 tagtgcacca gcagagcctg ccagcatcta tatgccgagg aacaagccag acagtacgcc
      481 cagaagcaat gcgagagagt tgaacaatag caatcctggt tttaagtgct gaaaggcaat
      541 ggatcccgag acggttttgt taatttataa aaaatgatat agtttcacta ttggtgcaac
      601 tatacttaat ttattaaact aacgtagcca ttctgtcatg accttttcta gtttctccag
      661 tgactgcgcg cgcttttcct ccgacatctg tccaatattg cgcagcttcc attgaacggc
      721 tggcaaattc tgaatgtggg gctctggcgc taaattccag tctggctcgc cgcgtttaaa
      781 tgacatcagg aagtcgaaat cctgttgagt gaaatgcgtt ttcaacgtgc tgagcattct
      841 ttccgggacc tcttttagct cttccagtga tacttcctca aaagtcatac cgctgaattc
      901 attttgaaag ggttcagtaa tatccttcca acgtgggtta agcacttcgg ataatgggcg
      961 gttatggctg agcaagtaca cgataaaacc attgaatatc gagcgatcaa ttccttgtgt
     1021 atcaaggagc attttgacgt cgtaaaaatc tctggggtgc tggcgatcta acgcagcaca
     1081 tagcttgcca ccgtataaat cgggcagtga gaccaccggg agcgcggcaa agccaaattc
     1141 ttcctccacg gctccgacga tatcgcgttc ttgcggagga tatagtgtcc cccgggcaac
     1201 tggcgagact tcaattttaa tttgcgcatc gtcagtggtg acaatgatgc gcatctcatc
     1261 gggattatgg gtttgcagca ctgcacggat cccggtttgc gcttccagtt ctgcggcgat
     1321 acgggtgagc gcctcccgca cgttgggcag cgcgacctct ctgctttcca gcggtatcca
     1381 ggccagatcg atatcaacgg acaatcgggg aaaatcatgg atgaataagt tgatcgccgt
     1441 tccgcctttc agcgcaaaac aggactcgtt agcgacccag ggtaatgctc gcaccaacag
     1501 agcgacctgg cggtagtagg gggaggtttt atccatggct tattcccttc ttagtaaaat
     1561 tttcgggcac agttatctgg tattgcgtgt tcagcttgcc attaacaacg atttgtcggt
     1621 tgcccgatcc caggttgatt ttgctggtat ctatgcgttt gaaccatgca tgttcataga
     1681 aactggcaaa aaacagatac aggcgcttgg tttgtacgga ctgactgagt gtaagcagct
     1741 tctccacttt acgtgggttg agattgacta gtccctgaaa aagctcggca gcgtgctcaa
     1801 atgagatttc ctgagggact gcgcttaaca gctcataggc agccagttct ggcgtgctgg
     1861 cggtaattct tttcccttta acctcaagct cagttcggta tttttcatcc aggtcaggga
     1921 gcttcagggc cgataccatc aaccactcaa cggcgggaaa ttctttaaac cattttggca
     1981 atatcgcttt gttttcaacg ctaagccagc attggttctg cttaagttgt aggtaatggg
     2041 aacggccctg ccaggagaga ctactcagtc cggccagatg caccggcgct tcaagttggt
     2101 tttgtaggca ccatagcgca tcactccagt ctggttctcg tcctgcgcgc gcataaacgc
     2161 cagcccgcag ttttattaac cagccatttt gcgcgtattt aaaagccaga gaaggtgata
     2221 tgttgttccg ggtcagccac gattgtaaca ccaggcttcc cggagaggtg ttttgcaaca
     2281 gccagtttaa ttttgatgcc atagattcac tcagagtgta actataataa aaaaagtaaa
     2341 ctatttagtg aataaaaagt caatgtatgg tgtctgtcgt agcataacgt tgtccacgaa
     2401 gcttttacat ggttaaaaca gcgttatggt gcccccttga ctgattaact gcgtgctcaa
     2461 aataccactt caatgtaaaa accgtggcta caagcctgcg ccgtcaggag cagagcgcaa
     2521 aagcctgccg gaatttcagc cctgtcaggt accgcgcaca cgaactgcct gtgtctagag
     2581 catgattttt acgtcagtgg cccgaaccag aagtgggcgg aggacttcac ttacttacgc
     2641 acagatgaag gctggctgta tctggcagtg ttaattgatt tgtggtcacg tgttttattt
     2701 ggctggccga gttagtttta atttgggcat tcgttgttat atacggttta tataccattc
     2761 ctcctaaagt aataaatcac aaaatgaagc atctcgaaga taaatatctt atttgatctt
     2821 tgcttctctg taacacctgt tttaacaaag caggt
//
LOCUS       DAZGTR010000268         2784 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGTR010000268 DAZGTR010000000
VERSION     DAZGTR010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.270"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..579
                     /locus_tag="ACHLCY_005026"
     CDS             <1..579
                     /locus_tag="ACHLCY_005026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372077.1"
                     /translation="KKNNVPMIQNNGNLQPFSMGNLPSIAKTFPMEIHTTILQQLNPL
                     EHISNAISRICYYKEQCKALELESLKIKEQASLMHLKIDTNFKKAMYELETRRKSNEL
                     KLIAAANDFKQISITKSDLIKEISKLVSSICSNDVSLESKKAMNDTLIIMKGMLSDQR
                     SESYEKFKSLLSSIDSSYNDALLIEDHQDNYV"
     gene            572..1153
                     /locus_tag="ACHLCY_005027"
     CDS             572..1153
                     /locus_tag="ACHLCY_005027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372078.1"
                     /translation="MFNIILGVAAVGVGLYILNELDDRAGEEKKRWENQRESVCKSIE
                     EHKRYIEQHVEYVQSMYDFHQLVDLHYSCMKVANEAYKLLNDAKKSMGAIYDAINKLK
                     AHRSKLFNDKKESDDYEKKKEIQAEINMIKEIIDGLYSELDLIKKDKEHFYQELKAFN
                     LKTSSIKYRIRDNTGYKGFDWYNRLQERTLLRR"
     gene            2134..2610
                     /locus_tag="ACHLCY_005028"
     CDS             2134..2610
                     /locus_tag="ACHLCY_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0372079.1"
                     /translation="MFIDSEKRLKQLSDEAKKSTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          904 a          549 c          625 g          706 t
ORIGIN      
        1 aaaaaaaata acgtgccaat gatacaaaat aatggaaatc tccaaccatt tagcatgggt
       61 aatttaccat ctattgctaa aacattccca atggaaatcc atacaactat acttcaacag
      121 cttaacccat tggagcatat atccaatgct ataagtagga tttgttacta taaagaacag
      181 tgtaaagcac ttgagcttga gtcactaaaa ataaaagaac aagccagttt gatgcacctt
      241 aaaatagata ctaattttaa aaaggcaatg tatgagctag aaacaagaag aaagagtaat
      301 gagttgaagt tgatagctgc tgcaaatgat tttaagcaaa tatcaataac aaaatcagac
      361 ttaattaaag agatatcaaa gcttgtttca agcatatgta gcaatgacgt ttcgttggaa
      421 agtaaaaagg cgatgaacga tactcttatc attatgaaag gcatgctcag tgatcaacgg
      481 tctgagtcat atgaaaaatt caaatctttg ttaagtagta ttgattctag ttacaatgat
      541 gcgttgttga tagaggatca tcaggataat tatgtttaat atcatactcg gagttgctgc
      601 tgtaggtgtt ggtttatata tcttgaacga gcttgatgat cgcgccggag aagaaaaaaa
      661 gcgatgggaa aatcaacgag aatcagtatg taaaagcatt gaagaacata agagatatat
      721 agaacaacat gtcgaatatg ttcaatcaat gtatgatttt catcagttag ttgaccttca
      781 ttattcatgt atgaaagtgg caaatgaagc atataaactt cttaatgatg ctaaaaaatc
      841 aatgggcgct atttatgatg ctatcaataa actcaaagct caccgctcta aattatttaa
      901 tgataaaaaa gaaagcgatg attatgaaaa gaaaaaagaa atacaagcag aaataaacat
      961 gatcaaagaa atcatagatg gcctatactc tgaattagat ttaattaaaa aggataaaga
     1021 acatttttat caggaattaa aagcttttaa cttaaaaact agcagcatta aatacagaat
     1081 tagagacaat accggatata aaggctttga ttggtataat cggttacaag aacgaacttt
     1141 acttcgacgt tagttttgca ctgaaactat atcagataac aatcctccac ggttccggca
     1201 gcgccttgag cgcaaccgga aaccgtctgg ggagaacccc agacccccat cgctgcaaca
     1261 tcgacggtct gcgaccacct gccaaccgag taactgcctg ttccgtgcga atgggctttg
     1321 cagggggctg gcgcggttcc tggcgacctg aaagcccctg gttaggggta tcggcgcgcc
     1381 tgacctcctg agagcctctg taacgcattt tcgcgcctcc catgccctgg cgggcattta
     1441 aaccccgttt cgtgcgtctg agagcgtttg aagggctatc tgacgactca tccccgaaag
     1501 aacaacgacg gaatcaccga caacatgcga gaggtggcac cgctcgccgc agccccagcg
     1561 accgaacgtt agtgagcgag tgggcgagga agcggaagag aaccggtagt tagattgagc
     1621 acgtacgcac tgatgggtgt cggggcgcag ccctgaccag atggcaaacg gaatatcgtc
     1681 gtgggtgacg gtattacatt tgcacatcct gtcccgattt ctgaggcgtt ttagccttca
     1741 tacccctttt agtggtgaac ggacggcaaa aacatgatgg aactgttgaa cgtggagagc
     1801 gcgaaatcgg acaaaaatca cggctgacga ggcttacaga ggagtaagcc agtatacact
     1861 ccgctatcgc tacgtgactg ggtcagggct gcgccccgac acctccaaag ggtgttgcgc
     1921 tcaacgcaac acccttgcct agaatagatc ttttacgccg atttgtaagt tgttgttttt
     1981 ttagtgtgtt ttttgtttac ctgttgcatc cagggtgaca caacgttgtc gcatcctgga
     2041 tgcgacaaca cgcaacaagg tgttgcacag atgtatcatg atggcttaga tttgaaccaa
     2101 cgttagtgca tgggattttc agagggaaaa atcatgttta ttgattcaga aaaacggctg
     2161 aaacaacttt cagatgaggc aaagaaaagc accgaggatc tcgaagaagc aaagaagaat
     2221 tcaaggttta cacaggtatc cccaaaaggt tgggaacgcg ttcgagagct gctgaaggat
     2281 agccaaggca tatcagcact gaagctgtac tcatttttag cggagcatat cgatcctacg
     2341 tgtggcgctg tcgttgcgga tcagcaattc ctagctgaaa aacttggagt tagcagaagc
     2401 acaattattc ggtggctcaa ttacttagaa tcaaaaaatg cattagttag aatccccgtt
     2461 gctggtaagg tttgtgcgta tgccctcgat ccacatgaag tctggaaggg atacaacact
     2521 acgaaaaacc atgcagcgtt tgtcactaaa acactggtca acaaagacgg tgatattcag
     2581 cgccgaatca tggccatgtt ttcaaattga gccagcggca ggcggacaat caggggctac
     2641 gtgttaacgt tttgaccatg attgtctatc ctgcattgct cttttaccac ctagacagca
     2701 ccatagcgat aagataaaca tgctcaaacg ttttatgtaa cattagaaaa attcagttaa
     2761 ttaaaaaagg ttttctaaat gagc
//
LOCUS       DAZGTR010000269         2731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGTR010000269 DAZGTR010000000
VERSION     DAZGTR010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.271"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..619
                     /locus_tag="ACHLCY_005029"
     CDS             <1..619
                     /locus_tag="ACHLCY_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP0372080.1"
                     /translation="ASGGLGKIPQDTLSGIRTLAFNGGAQVQMGSTIVTIRSVFLGFF
                     MGSVSPEGLSERALRTALNNVNRLERDLNGGLSGRQILASIPSPYASPPRPSVMQTEL
                     VLEQIEKCSFNVNSSSLRATEEALTCPITLCIPEHGVIMRNAGDSDVCTLYDKESLKH
                     LVNTSSPHPLSREKITESMIVKENSCYFDFTSGSIKNVRGEITRL"
     gene            complement(879..1098)
                     /locus_tag="ACHLCY_005030"
                     /pseudo
     CDS             complement(879..1098)
                     /locus_tag="ACHLCY_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1313..2635)
                     /gene="nleA"
                     /locus_tag="ACHLCY_005031"
     CDS             complement(1313..2635)
                     /gene="nleA"
                     /locus_tag="ACHLCY_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFP0372081.1"
                     /translation="MNIQPIVTSGITTQNNRHHHAEQTSPTQIPQSELPNGCETGFVV
                     HIPEDMQRHAPECGETTALLSLIKDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     SQMALEIPSTVPGISGKYGVQLNIVKPDIHPTSGNYFLQIFPLHDEIGINFKDLPGPL
                     KNALSNSNIPTTVSTAASTIASATTSTVTTASKDPIPWFGLTAQVVRNHGVELPIVKT
                     ENGWKLVGETPLTPDGPKANYTEEWVIRPGEADFKYGASPLQATLGLEFGAHFKWDLD
                     NPNTKYAVLTNAAANALGAVGGFAVSRFTSTDPMLSPHIGAMVGQAAGHAIQYNTPGL
                     KPDTILWWAGTTLGLADLNKAETGEGRFTDYPRIWWHAREGAIFPNKEDIARATGADI
                     RAMEEGIPVGQRHPNPEDVVIDIESNGSPHHNPSNYVDTFDIIQETRV"
BASE COUNT          756 a          524 c          598 g          853 t
ORIGIN      
        1 agcgagtggg ggactgggga aaataccgca ggatactctt tctggcatac gaacactggc
       61 ttttaacgga ggagctcagg ttcagatggg gagcactatt gttacaatac gttctgtctt
      121 tttaggcttc tttatgggaa gtgtaagccc tgaaggatta tcagagagag ctttacgaac
      181 tgcattgaat aatgttaatc gtcttgaacg cgaccttaat ggagggctat ccggccgcca
      241 gattttagca agtataccca gcccgtatgc ttctccacct cgtccttctg taatgcaaac
      301 agagttggtg ttagagcaaa tagaaaaatg ctcatttaat gttaactcgt catctctgag
      361 ggcgactgaa gaggcattaa catgcccgat tacattatgc atccctgagc atggtgtgat
      421 tatgagaaat gctggagatt cagatgtatg tactttatat gataaagaat cattaaaaca
      481 tcttgttaac acaagttcac ctcatcctct gagcagagaa aaaataacag agtcaatgat
      541 tgttaaggaa aactcgtgct attttgattt cacatcagga agtattaaaa atgttcgtgg
      601 tgagataact agactataag atgttagaac atattgactg ataagtgtaa ttcacagaca
      661 tcttttcagt tttttctgtt aattttctaa ttgatgggct gtatacaggg ataaaaggag
      721 agggtattgt ctggacattt ctgttagcat aattggaaat gtgcgtgaaa aaaagcataa
      781 cagacatcat gtgttatgta tgtattcaga gtgcccataa ttcaccagtg cagggataaa
      841 ttgtaaaaaa atcgcagtac gtcgtatgca gaacggtgcg gcgtacgcag ttccagtgga
      901 ccgtcgcctg tgataacggt ctttgtggaa taaccgttgc gggagttagc tcctgatctg
      961 gactgatttt tctcattccc aagatggtgt gtcatctctg cattgagagc ggcttcaacg
     1021 ctgagctttt tcagcaaccg atcaaactga ctgaggtctt ctgagggggc tgaggttttt
     1081 ggccagttcg ttagcaatac accagaaaaa ttctaaacga gcctgtacat agatttgtgt
     1141 aattgcctga ttttgatatg ttcaacccaa catcaaatga aggttaattt atggatgaaa
     1201 aacagttaca ggctatttgt tttgtgggat ttttaggaaa aagatatatt aatccttatt
     1261 aataatagct gtcatccatt tcagctatta tttttaaata aataagtaaa gcttagactc
     1321 ttgtttcttg gattatatca aaggtatcaa cataatttga tgggttatga tgtggtgagc
     1381 cattgctttc gatatcgatg accacatcct ctggatttgg atgtcgctgt ccaacgggta
     1441 taccttcttc catagctcgt atatcagccc ctgttgcacg ggcaatatct tctttattag
     1501 ggaagatggc accttctctt gcgtgccacc atatacgagg atagtcagtg aatctgcctt
     1561 cacctgtctc ggctttgttt aaatctgcca gccccagagt cgtacctgcc caccataaaa
     1621 tagtgtctgg ctttaatccg ggggtattat actgtatggc atgccccgct gcttgcccaa
     1681 ccattgcacc gatatgagga cttaacattg gatctgtact agtaaatctg gatactgcaa
     1741 atccccctac agcaccaagc gcatttgcgg cagcattggt aagaacggca tatttagtat
     1801 tagggttatc taaatcccac ttgaaatgtg cgccaaactc cagccctaga gttgcctgta
     1861 gtggagatgc accatattta aaatctgctt ctcccggtct gatcacccac tcttcagtat
     1921 aatttgcttt ggggccatca ggagtaagag gagtttctcc aacaagcttc catccatttt
     1981 cagttttgac tataggaagt tccacaccat gattacgaac tacttgagct gttaatccaa
     2041 accatggtat tgggtctttt gacgcggtgg ttaccgtcga agtagtggct gatgcaatag
     2101 tggatgcagc agtcgataca gtggttggta tattgctgtt gcttaatgca ttttttaatg
     2161 gaccaggaag gtctttaaaa ttaataccta tttcatcatg caaagggaat atctgtaaaa
     2221 aataattacc tgatgtagga tgaatatctg gttttacaat gtttagctgg acaccatatt
     2281 taccagagat acccggaacg gtgctgggta tttctaatgc catctgagag acattgaata
     2341 aaccaaacat atccaatgct ttttttcctg cagagccttc ttcaagatga ggtgcaagat
     2401 atttatccag cccagagagc agaccttcat cttttatcaa gctcagtaga gctgttgttt
     2461 caccgcattc cggtgcatgt cgctgcatat cctctgggat atgaacaaca aatcccgttt
     2521 cgcatccatt aggtaattcg gattgcggta tttgtgtagg ggacgtttgt tctgcgtgat
     2581 gatgtcgatt gttttgtgtg gtgattccgg atgttacgat cggttgaatg ttcatgataa
     2641 atccttattg catgtttata tccattaata atatcaatcc attgatatta ttagtgtcac
     2701 atatccgatg tggacagctt taatatatag c
//
LOCUS       DAZGTR010000270         2713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGTR010000270 DAZGTR010000000
VERSION     DAZGTR010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.272"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            84..1058
                     /gene="cysB"
                     /locus_tag="ACHLCY_005032"
     CDS             84..1058
                     /gene="cysB"
                     /locus_tag="ACHLCY_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP0372082.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1377..1517
                     /locus_tag="ACHLCY_005033"
     CDS             1377..1517
                     /locus_tag="ACHLCY_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP0372083.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            1520..1687
                     /gene="yciX"
                     /locus_tag="ACHLCY_005034"
     CDS             1520..1687
                     /gene="yciX"
                     /locus_tag="ACHLCY_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP0372084.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1801..1896
                     /gene="ymiC"
                     /locus_tag="ACHLCY_005035"
     CDS             1801..1896
                     /gene="ymiC"
                     /locus_tag="ACHLCY_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP0372085.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2060..>2713
                     /locus_tag="ACHLCY_005036"
     CDS             2060..>2713
                     /locus_tag="ACHLCY_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFP0372086.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
BASE COUNT          702 a          629 c          639 g          743 t
ORIGIN      
        1 tttttattat taaatcgtat tagtcacccg ccaggtgtga cgaaaaaacg atgttctgat
       61 ggcgtctaag tggatggttt aacatgaaat tacaacaact tcgctatatt gttgaggtgg
      121 tcaatcataa cctgaatgtc tcatcaacag cggaaggact ttacacatca caacccggga
      181 tcagtaaaca agtcagaatg ctggaagacg agctaggcat tcaaattttt tcccgaagcg
      241 gcaagcacct gacgcaggta acgccagcag gacaagaaat aattcgtatc gctcgcgaag
      301 tcctgtcgaa agtcgatgcc ataaaatcgg ttgccggtga gcacacctgg ccggataaag
      361 gctcgctgta tatcgccacc acgcataccc aggcacgcta cgcgttacca aacgttatca
      421 aaggctttat tgagcgttat ccccgcgttt ctttgcatat gcaccagggc tcgccgacac
      481 aaattgctga tgccgtctct aaaggcaatg ctgatttcgc tatcgccaca gaagcgctgc
      541 atctgtatga agatttagtg atgttaccgt gctaccactg gaatcgggct attgtagtca
      601 ctccggatca cccgctggca ggcaaaaaag ccattaccat tgaagaactg gcgcaatatc
      661 cgttggtgac atataccttc ggctttaccg gacgttcaga actggatact gcctttaacc
      721 gcgcagggtt aacgccgcgt atcgtcttca cggcaacgga tgctgacgtc attaaaactt
      781 acgtccggtt agggctgggg gtaggggtca ttgccagcat ggcggtggat ccggtcgccg
      841 atcccgacct tgtgcgtgtt gatgctcacg atatcttcag ccacagtaca accaaaattg
      901 gttttcgccg tagtactttc ttgcgcagtt atatgtatga tttcattcag cgttttgcac
      961 cgcatttaac gcgtgatgtc gttgatgcgg ctgtcgcatt gcgctctaat gaagaaattg
     1021 aggtcatgtt taaagatata aaactgccgg aaaaataatt tcttgcgtta ttttcggcac
     1081 cttttatgta gcgaaggtgc cggaatatat tctcttttgt tacttatttc tataccgtgc
     1141 acaatatttt tatccctcat atttataggg taaattacct gtaaagtagt gtctaattac
     1201 ccgcctcgca aactccagtt ttcaaatatc tatttcaggt caaaagattg aattattcac
     1261 tggagacgat tcgtaaattc gctggatttt tgactaaagt ttctttagga tttgtctcat
     1321 cgtatgatta attacaccaa tcgttcaacg tcttgctgat aagtgatggc gattatatga
     1381 ggttagcaat gccttctgga aatcaggaac cccgccgcga tcctgaatta aaacgtaaag
     1441 cctggctggc ggtttttctt ggttctgcac ttttctgggt ggttgtcgca ctgctgattt
     1501 ggaaagtgtg gggataacta tggtcggtca ggagcaactg gagtcgtcac cattatgcca
     1561 gcatagtgac aatgaaacag aaacgaaacg ggaatgttcc gtcgttattc cagacgactg
     1621 gcaactaaca tcgcagcagc aagcctttat agaactgttt gctgaagatg atcagccgaa
     1681 acaataatta tcatcattct tattacccat ttttaatgaa ttaaagggct tttaatacac
     1741 cgcagcaata acagcttgag ttatctcaac acaaaataat aaccgttaag ggtgtagcct
     1801 atgatcaaca caaatatgaa atattggtcc tggatgggcg cgttttctct gtcgatgctc
     1861 ttctgggccg aactcctctg gatcattact cactgatcct tgaccccgct gcggcggggt
     1921 tgtcatttgc tttgccacaa ggtttctcct cttttatcaa tttgggttgt tatcaaatcg
     1981 ttacgcgatg tttgtgttat ctttaatatt caccctgaag agaatcaggg cttcgcaacc
     2041 ctgtcattaa ggaggagcta tgtcgtcaac cctacgagaa gccagtaagg acacgttgca
     2101 ggccaaagat aaaacttacc actactacag cctgccgctt gctgctaaat cactgggcga
     2161 tatcacccgt ctacccaagt cactcaaagt tttgctcgaa aacctgctgc gctggcagga
     2221 tggtaactcg gttaccgaag aggatatcca cgcgctggca ggatggctga aaaatgccca
     2281 tgctgaccgt gaaattgcct accgcccggc aagggtgctg atgcaggact ttaccggcgt
     2341 acctgccgtt gttgatctgg cggcaatgcg cgaagcggtt aaacgcctcg gcggcgatac
     2401 tgcaaaggtt aacccgctct caccggtcga cctggtcatt gaccactcgg tgaccgtcga
     2461 tcgttttggt gatgatgagg catttgaaga aaacgtacgc ctggaaatgg agcgcaacca
     2521 cgaacgttat gtgttcctga aatggggaaa gcaagcgttc agtcggttta gcgtcgtgcc
     2581 gccaggcaca ggcatttgcc atcaggttaa cctcgaatat ctcggcaaag cagtgtggag
     2641 tgaattgcag gacggtgaat ggattgctta tccggataca ctcgttggta ctgactcgca
     2701 caccactatg atc
//
LOCUS       DAZGTR010000271         2711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGTR010000271 DAZGTR010000000
VERSION     DAZGTR010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.273"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            32..586
                     /gene="ybeT"
                     /locus_tag="ACHLCY_005037"
     CDS             32..586
                     /gene="ybeT"
                     /locus_tag="ACHLCY_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0372087.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(596..2023)
                     /gene="djlB"
                     /locus_tag="ACHLCY_005038"
     CDS             complement(596..2023)
                     /gene="djlB"
                     /locus_tag="ACHLCY_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP0372088.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2020..>2711)
                     /locus_tag="ACHLCY_005039"
     CDS             complement(2020..>2711)
                     /locus_tag="ACHLCY_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0372089.1"
                     /translation="KILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGIDAR
                     DDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTAEC
                     CGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAGYT
                     FGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLAYYSA
                     CPETLKDMSDL"
BASE COUNT          786 a          644 c          601 g          680 t
ORIGIN      
        1 caaaataaaa agaaaatcga agcattcaca tatgaataaa aaattaatgt atatattcgc
       61 aatatttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacaa cgttgcgtga
      121 taaagccatg gtgaattatg cctttgatta tttaagctca ccgggcagtc taccgtttac
      181 cacggcggcc acggagcttt ccgcgattca tggtcactca acgtcgcaat atcgccttgg
      241 agaattttat cttcatggta gcgacggtaa accactggat tatacacagg cgagatactg
      301 gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg ggtggatcta
      361 cctcaaaggt ctgggggtca aacccgacac ccgtaaagca attctctggt ataaggaagc
      421 agctgaacaa gggtatgctc atgctcaata tactttaggt ttgatctaca gaaatggctc
      481 aggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg ccaaacaaca
      541 ttacaaaaat gcggaaagat tacttgccgg gcttcccgca cattaacgtc gactgtcaac
      601 cgaacagatg gagtattttc cctatcaaac cagcaaagat aaaaatatag aaaataattt
      661 taatgatgcc cagacagcct ggcccaccat cgtcaacggg cattggctgc ggttgcggct
      721 ccggttcaac agtttgcttc tcggtaaaat tcgccgcctg aattaataat cgacgaataa
      781 ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc ggcattaatg
      841 attcttcggt tggttggttc gcctgcacta attgtttggc aactaaatat aatggccata
      901 agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg tgcaaaacca
      961 tctcgaccag tgaacgcccc agagcagaga gtttttcatc gctgagtatt tcgtataaat
     1021 gaaaacgcgt acgggcgctg tcgccgggga tataaagtag cggttttccc tgatcgttaa
     1081 ccagttcacg agcttcaatc atcatcacca ctaacgggta ataatcgacc cgatgctgac
     1141 ggcaccattg caatattaaa gcatccgcct gggtggaggg atactcgcgc cagtagtcac
     1201 aaagttctgc cagcaagctt tcgccttctc cacaatagac gcgttgtgcg tattcataat
     1261 aacgcgcatc ttcattatca gattcagctt cccgccacga ccgggcgtaa tccaggagtt
     1321 cgggaattcc caggcgacct gcgctatacc agcggagtaa atttctgtgc agttttgcat
     1381 catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat tctggatgcg
     1441 atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc acggcaacgg
     1501 gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca tataaaaagg
     1561 attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac cgcaatcttt
     1621 ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg attgtgcaca
     1681 gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc tgaatgaatc
     1741 gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgca cgaaaggcga
     1801 gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat ttagcaggcg
     1861 actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa ccctgcggat
     1921 cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata atatcgacgt
     1981 cagtcgtttc ctctatttcg agaatcttcc agcaattttt cataggtcgc tcatatcctt
     2041 aagcgtttcc gggcatgcag aatagtaagc caatggttgc caggacaagg tagggtaata
     2101 cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt cgtattcttt
     2161 acgcgctaag aattcgcgta ataactgcag atgatcatct tcgggagact gccaatattg
     2221 ccgaccaaag gtataaccgg caaagtattg ccgccagtta cgataataac gtagagcccg
     2281 tagatgaatg cgcgactgga tccatagact ttcttcttcg ctcaaccagt tattaagtac
     2341 gcccattcgg cttaaaaaac ccatccggac ataatcccag gctttaattc ctccctcacc
     2401 acagcattcg gcggtgctgg cgacaaattg cgcataagcc tgaccttgct catttagttc
     2461 agcaagatag tcacgccatt cacatggcga ataacgaaac cagcggtgat aaaaccctgc
     2521 caaccgagcg gcatgaccgt tatcgatcat tcggtgaatt gtgcgcagca ggtcatcacg
     2581 ggcatcaatt ccccaggagg aagaaaggtc aaaaaagcat gtacctaaat acattttggg
     2641 tgatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg aaagtgcaaa
     2701 caatattttt t
//
LOCUS       DAZGTR010000272         2710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGTR010000272 DAZGTR010000000
VERSION     DAZGTR010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.274"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..302)
                     /locus_tag="ACHLCY_005040"
     CDS             complement(<1..302)
                     /locus_tag="ACHLCY_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0372090.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVISTPEGEWYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDI"
     gene            complement(308..1006)
                     /locus_tag="ACHLCY_005041"
     CDS             complement(308..1006)
                     /locus_tag="ACHLCY_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0372091.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(1006..1347)
                     /locus_tag="ACHLCY_005042"
     CDS             complement(1006..1347)
                     /locus_tag="ACHLCY_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372092.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEPTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(1340..>2710)
                     /locus_tag="ACHLCY_005043"
     CDS             complement(1340..>2710)
                     /locus_tag="ACHLCY_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0372093.1"
                     /translation="DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDSYQ
                     EARQRYISNALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTEL
                     AKLKYQTAQGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNE
                     AELLGYGQGERARERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYL
                     SERLKDQAAFYAESDAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNI
                     TEMLNGNKVEWRNWASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFLGSIGDWLGG
                     AVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLG
                     VRAVGSMNGSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRD
                     GGMLSG"
BASE COUNT          529 a          937 c          618 g          626 t
ORIGIN      
        1 tgaatgtcac cccggcagac cagccaccag ggcagtgcgc tttttatctg cagccgccgg
       61 tcagcctcgc tcagccaggg cagcccaccg ggatgactgt ggaccagtgc cacaatctcc
      121 ccctgcatct ctgcccgcag ccagtcttcc ggtgcgatac gaaaatacgc ctccggctct
      181 gcagaaatat tcacacaagg gatataccac tccccctccg gcgtgcttat cacgaagccg
      241 cacgactccg caggcacaca ccgccgggca tgcgccagaa tcgctgattc tgtctgtgtc
      301 attggattta ctgcgaaagt ttgttaatgg aaaggaaacc gccaaaatta gccaccatgc
      361 cgcgcatctc acacccgcgc atgcacttgc tgcatctgtc cttacggata tcggtggtgg
      421 gtttatcgaa ctcatccgcc accgcaggac cgttataccc gcattcatct ccccggtaat
      481 cccacataca ggtgttcgcc agcatgatgc gaccgggaaa cagcgcccca tccgtctcgg
      541 tcggtgtagc cagcacaaac gaggccgtca tggctgtcag ctgcgacatc tgctccacca
      601 cccagcggtc actcagctcc tgctccgggt ccgcttccgg attgcccgca acgaaattca
      661 ccgcatccag aaaacgggca tacacccggc ggcggaccac cgtggccccc accagactct
      721 gcaggtcctc cgccatcccg gtgacaaggc caaacagatt ggacaccgtc agcgacgggc
      781 gggcactgct gccccggccg ttcatctcaa agccgctgcc gtcaatcggg tatgcctcat
      841 acttacgccc ctgccaggtg accggctccc ctttttcatt cagctcatta cagaaaaaat
      901 accgctcacc accctgtacc gtcaggtcga tttcccagag taccacccgc ggtgactgct
      961 ctgacttaac cgactcgttc agactttctt cgcgaatatc ctgcatcagt tcaccacctg
     1021 ctcaatcgta caactgaaat cactgtacct ggcgttatct gtgacgctcc actcccggca
     1081 caccaccctc accgtccggt tatgtttcgg cggtcgccac aaaaaggcac ggtaaccacc
     1141 atgccaggat aaaaattcat ccagccagcg ccgggttggt tcatccgtca cccggaacac
     1201 cgcctgaaac gtcttcagtc tggcattaag tcccgtcggt cggcgctgtt cataaccgtc
     1261 accaaaccga accctcacca ccgacggttt ctcactcacc tgcatccctt cacgcgggac
     1321 cagatgcagc gtttttatct cagccactca gcattcctcc gtcacgtcgc atggacagca
     1381 tcaccgcctg cacccgctgg tcaatcagct gcacaagact gcctgccgcc tccggcccta
     1441 tctggccatt agtcccgtca ttctgaatgg cgatatggta gaccggggaa tacaccagac
     1501 ccgcactgcc gttcatactg cccaccgcgc gtacgcccag cgagccatcc gccgcccggg
     1561 tcaggggcat aatagcttca ggtccggctt cccccatcag cccggcccct tttgcaaacg
     1621 caaagtacgt gggcgtatcc acaatactgt tgctgtacgc actcaggttt gccgaggtat
     1681 acacgccgcc ttttgcattg gccaccgccc cgcccagcca gtcaccaatg ctgccgagaa
     1741 atcctcccgc accggacata ccgtttgccg ccgtcttaat tccgttgaca atcgcggcat
     1801 tcataagaac ttttgagatt tcctgcagta cggatgaggc ccagttgcgc cattccactt
     1861 tgtttccgtt cagcatctcc gtgatgttat tcaccatccc tgagataccc tccgtcgcaa
     1921 gctgtgctgc ctgtgaagcg taatcggatg cattgtccac ccagttactg agcccctcct
     1981 gcaagccttt ctgccagtcc gcacgctgcg catccgattc ggcataaaag gctgcctggt
     2041 ccttaaggcg ttcgctcaga tactgcgcgt tctgtgcccg tgcctgtctg taaaaatcct
     2101 cactgatatc cccggtctga tactgagact gaaggtccgc atccttctgg cggaagctgt
     2161 cgcggatctg ctgcaactcc cgcatgcgtt ccctggctcg ttctccctgc ccgtatccca
     2221 gcagttcggc ttcatttgat gcacgcgcag ccacattatc attcttcagg gtctcttccc
     2281 gggatcgcaa ctgttcccgg attttttgct ggtcaatcag ggccgcgtta cgcagcagtt
     2341 cctgcttctg catctccgtc agggttttca gttcgccctg cgcagtctgg tacttcagct
     2401 tcgccagctc tgtattctga cccgccagtg ccagttgctc tttctgctgc ttcagtagcc
     2461 gggaaaaact gtcttccgct ttttccgtct ctgattttcc accccgggat ttaggtttgt
     2521 tcgcctcgtt attacgccag gcttccagag cattactgat ataacgctgt ctcgcctcct
     2581 gatacgaatc ccccacaaaa ccaaggtcat ccgccgcata ccccagccgg acacgctctt
     2641 tttcctcccc cttcagtcgg gacagggcca gctcacgttc tgtttttgtc agggcgctct
     2701 gctgtttatc
//
LOCUS       DAZGTR010000273         2653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGTR010000273 DAZGTR010000000
VERSION     DAZGTR010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.275"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..420
                     /locus_tag="ACHLCY_005044"
     CDS             <1..420
                     /locus_tag="ACHLCY_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372094.1"
                     /translation="RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMY
                     DRMTEVVQYHAELKSLRGSERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYAD
                     STLSLAQQSAMVKSVERDMKIAVDRFNREYNKKVGGE"
     gene            complement(643..1575)
                     /locus_tag="ACHLCY_005045"
     CDS             complement(643..1575)
                     /locus_tag="ACHLCY_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP0372095.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1869..2624
                     /gene="mlaA"
                     /locus_tag="ACHLCY_005046"
     CDS             1869..2624
                     /gene="mlaA"
                     /locus_tag="ACHLCY_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP0372096.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          656 a          611 c          686 g          700 t
ORIGIN      
        1 cgcttcattt ccaaaaccac ggatgcggcg gtgaaatcgc tgaatggtat tgatataccg
       61 gagcagcagg tgcctttcct ggggaaaatt tcgggagagg tgatgccgta tgctgaccag
      121 cagaagatgt atgaccggat gacggaggtt gtgcagtacc acgcagagct gaagagcctg
      181 aggggttccg aaagaacggc attcattgac gagaacaacg gaaaattgtc gatgaacggg
      241 cttatgcagg atacccggaa gagactgaag gatttgcgta aacagcgtga tgccatttat
      301 gccgacagta ctctcagtct ggcgcaacag tcggcgatgg tgaaatcggt tgagcgggat
      361 atgaagattg ccgtggatcg gtttaaccgc gagtacaaca aaaaagtggg gggggagtaa
      421 gagaaattgc cccgtactga agtgcggggc attcttaaca taatgtgtac ataagtgagt
      481 actgttgtga tggatgtttt ggtatttaat gcatttaaat caatggattg atatttatgt
      541 tcattatatc catttaacta agaggacaat gcggcatgag tatacccgct aatggactgc
      601 ggggtaagta cgctgccgct cgattgctta aaccctcgcc atttatgctg ggtttttatc
      661 atttttctta atgttttccg cacgttctgc tttttggcgt gcttctgctt tacgcttgtt
      721 gctcatgtcg ttacgaatct gtgcatgact cattaacgcg aagataaagg tgccgccgca
      781 gatgttcccc gctaaagtag gtagtgcgaa gggccagatg aaatcgctcc agtgcagcgt
      841 gccgttaaac accagataga ggatttcaac agaaccgaca acgatgtggg tggtgtcacc
      901 cagtgcaata agccaggtca tcaaaataat caccacaatc tttgccgcac ccgctgcagg
      961 aaacatccaa accatagtgg cgatcagcca gccggaaatg atcgcgttgg caaacatctc
     1021 gctgggggtg ttcttcatca catccatgcc gattttgaca aatgcatcgc gagtttcttc
     1081 attgaagata ggcatatatt caaatgccca tgcagcaata cctgtcccga gaatattacc
     1141 cagcagcacg acgccccata atcgcataag taagccgacg ttgctcattg tcggtttttg
     1201 catgacgggt agtaccgcag tcacggtgtt ttcggtaaat aattgctggc gggccataat
     1261 gacgataata aaaccaaagg tataaccgag attctccagt aagaagctgc ctggcactcc
     1321 ttccagttcg acatgaaata tcccttttgc cagtagcgaa gcgcccatcg acagacccgc
     1381 cgcaatggct gaccacagta gcgccattgc gtcgcgttcc agctcttttt caccatcctg
     1441 gcggatatgc tcatgaattg ccatcgcccg ggaggggagt cggtcttcat ctatttctat
     1501 ttttttgccg cgctcttttt cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat
     1561 tttgtcgttg tccatggtct cttctcttga attagcacgt atagctaaag cgtagcggct
     1621 tttttgctcg caactggcgg gagttactct gaaaatgtag aaaaggctgc gtttgccttt
     1681 ttctgtttct atagaatcaa gtagcctaca gggcggcgat taccaggcta tgatcaaatc
     1741 agcaaatcag ggcgtctgga catcagttga cgtgctgtta caatcgccca cacctaaaca
     1801 ggcggatacg gtatcgttcc gtcatggatg gcaaactgca taagccataa aaaaacaggg
     1861 agacatttat gaagcttcgc ctgtcggcgc ttgctctggg aactacgctt ctggtggggt
     1921 gtgcgagttc cggtacagat cagcaagggc gttctgaccc gttagaaggg ttcaaccgca
     1981 ccatgtacaa cttcaacttc aacgtattag acccgtatat tgttcgaccg gtcgctgtcg
     2041 cctggcgtga ttatgttccg caaccggcgc gtaatggttt gagcaacttt actggcaacc
     2101 ttgaagaacc tgcggtgatg gttaactact tcttgcaggg cgacccttat caggggatgg
     2161 tccactttac ccgctttttc ctgaacacca ttttggggat gggcggcttt attgatgttg
     2221 cagggatggc gaacccgaaa ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg
     2281 gtcattatgg cgtgggttat gggccttacg ttcagttacc gttctacggt agcttcacgc
     2341 tgcgtgatga cggtggtgat atggcggatg gtctttaccc ggttctttcc tggctgacct
     2401 ggccgatgtc tgtgggtaaa tggacgcttg aagggatcga aacccgcgct cagctgctgg
     2461 attccgatgg tctgctgcgt cagtcgtccg atccttatat tatggtgcgc gaagcgtact
     2521 tccagcgtca tgatttcatc gctaatggcg gcgaactcaa accgcaggaa aatccgaacg
     2581 cacaagcgat tcaggatgat ttaaaagata ttgattctga ataagaaaca aataaaaaag
     2641 gtgagtcgca aaa
//
LOCUS       DAZGTR010000274         2652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGTR010000274 DAZGTR010000000
VERSION     DAZGTR010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.276"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            13..231
                     /locus_tag="ACHLCY_005047"
     CDS             13..231
                     /locus_tag="ACHLCY_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP0372097.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            233..790
                     /locus_tag="ACHLCY_005048"
     CDS             233..790
                     /locus_tag="ACHLCY_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0372098.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            1081..1236
                     /locus_tag="ACHLCY_005049"
     CDS             1081..1236
                     /locus_tag="ACHLCY_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0372099.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            1356..1700
                     /locus_tag="ACHLCY_005050"
     CDS             1356..1700
                     /locus_tag="ACHLCY_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFP0372100.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            1822..2094
                     /locus_tag="ACHLCY_005051"
     CDS             1822..2094
                     /locus_tag="ACHLCY_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372101.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            2096..>2652
                     /locus_tag="ACHLCY_005052"
     CDS             2096..>2652
                     /locus_tag="ACHLCY_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0372102.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT          692 a          600 c          722 g          638 t
ORIGIN      
        1 gggcggtaag aaatgaaaca ttatttagaa aaaaattacc cacgaaagag cagaacaaca
       61 gagtttctgt ttttcattct gtttatagtg ttgatgatac cgatatcccc gctattactg
      121 gtctggataa ttggaaggac atttgaacca gttattgagc tatataccga tgtgacatgg
      181 gaatcattca gcgcactgca caataaaatt aatccgtata aggaaaactg atatgagcac
      241 tattaccaga gaacgcgcgg agattaaatc atacatcaca ggcttcctga gcgactcggc
      301 gcacgataac aagtcttcag acagcctgct ggctaatgtg tttcgtatcg cgctggcatc
      361 actggaagca gagccgatag caatggtagt gcctgatgaa atggatttgc ttacctgcca
      421 tctcgacggt gtaactaaaa catatgctga tggctggaac gcctgccgcg tcgccatgct
      481 tcaggccgga aactttcggg aaaataagaa ttcgtcaacc aacaattttc gggaaatctc
      541 ggaaacgtca accagatctc cgataactct ggatggctgg ataagctgta ctgagcgaat
      601 gcctgaaaag agccagaacg tgcttatttc gatgaatatc gatagcgagg ctgggccatt
      661 aatatattcc gcacgctatc tcggaggcac gttccggcgc ggaggtatag cagttagtcc
      721 gggtaatgat cttaggcaag caacccactg gatgtcgcta ccagaaccgc cgcaggaggt
      781 gaatcaatga cctggcctga agcattcaca acggtaggaa ttgcgatggc ggtggcgctg
      841 gtggagtatt cgatttgccg ctggggataa aaacggtttg cgggaaaagg atagttaagt
      901 agaattgcag cgggtgcttg aggctatctg cctcgggcat gaacaccaac ggcagataga
      961 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggctcaa
     1021 tccatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
     1081 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg
     1141 acggcactgg taacgaggaa agacctctgc gaggtgcgaa tccgaaccgg tcagacggag
     1201 gtcgctgtct tcgtagacta cgaatctgag aagtaagaga ccaggcgggg gagtaatctc
     1261 ccgccacctc tgatgtgtca ggcatcctca acgcacccgc gctttaccat actgaaaatg
     1321 ctgtttgaat gttcatctct gaaagaggac tatgaatgaa aaaggtattg attgcagcac
     1381 ttatttccgg tgtgtctttt ggcgcttttg cacagcaggg tggtttccag gggccagaag
     1441 cagagcgttc aacagtagcg caggcaaaag aactgaagga tgatgcatgg gttatccttg
     1501 aagggagcat cgttaaaaaa gtgggtgatg aacgttatga gtttcgtgac aatagcggga
     1561 caattgtcac ggatattgat gacagcgtat gggccgggca gaatgtttct ccgaaagaca
     1621 aagtaagaat tgagggtgaa attgataaag acctgagcag tgttgaagtt gatgtaaagg
     1681 cactgaaatt attaaagtaa ccgcccctgc ttgttaagcc cgtcttactg acgggttttc
     1741 tgtttgtaca ttccggcgta ttgccttaca attcgcgcag tcagcctgaa caactgacac
     1801 ctgctgtcac cggagaatcc gatgacacaa cacataaaat cccacaattc tgaagccgac
     1861 ccggaaatta agcaggggag gcgttttcgt gcgcctcagt atggctggtt tcactatctg
     1921 ttctgtacga tcgatgaggc agatatgctt caagaggcgt atctgcgtcg cggtgtccgt
     1981 gtggagcgga gtctgaacgc tgatcgtctg acctggaccg tttctgtata tcttcctgtt
     2041 cgtgcacatc tgccacggac acatgcctgc taccgtcagc gcgtctggag gtaatgtgcg
     2101 ggtattactt cgacctgttc tggttccgga actcgggctg gtggtcctta agcccggtcg
     2161 tgaatcattg ccagtttttc atcgcggcag ggtgctggtg gagccggaac cgaaaaacat
     2221 gcgggcgctg ccatctggag cggttcctgc tgttcgccag ccgctggcgg aagataaatc
     2281 actgctgcca tttttcagcg atgagcgggt gattcgtgca gctggcggcg ctggtgcact
     2341 gtctgactgg ttattacgtc acgtgaaatc ctgccagtgg ccacacggcg attatcatca
     2401 cagcgaaacc gttattcaca gttacggtgc tggcgcaatg gtgttgtgct ggcactgcga
     2461 caaccagctg cgcgaccaga cctccgaatc acttgagcaa cttactcaac aaaatctgac
     2521 agcctggatg attgacgtca tacgccatgt aatgaatggc acgcaggagc gggaattatc
     2581 gctggctgaa ttatcctggt gggcagtctg caatcaggtg gtggacgcat tacctgaggc
     2641 agtatcgcgt cg
//
LOCUS       DAZGTR010000275         2633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGTR010000275 DAZGTR010000000
VERSION     DAZGTR010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.277"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..924
                     /locus_tag="ACHLCY_005053"
     CDS             <1..924
                     /locus_tag="ACHLCY_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0372103.1"
                     /translation="TEEVMLDKITGKTPVAAYVSPIVEGKVLRHRGGETRVLRPGYVK
                     PKHEFNYQQAVERLPGEDPAQLNDPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLY
                     GKYTMEGDQFDTVEVDFGRSEGNNIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLV
                     NIAIMDGTVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVA
                     KTSYVAEDGTEKRYLPEGMLVLGNTAAEGIRCYGAIKDAQALSEGVVASSRYPKHWLT
                     VGDPSCEFTMTQSAPLMVLPDPDEFVVVQVK"
     gene            976..1359
                     /gene="gpFI"
                     /locus_tag="ACHLCY_005054"
     CDS             976..1359
                     /gene="gpFI"
                     /locus_tag="ACHLCY_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0372104.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            1352..1705
                     /locus_tag="ACHLCY_005055"
     CDS             1352..1705
                     /locus_tag="ACHLCY_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0372105.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            1721..2254
                     /locus_tag="ACHLCY_005056"
     CDS             1721..2254
                     /locus_tag="ACHLCY_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372106.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
     gene            2251..>2633
                     /locus_tag="ACHLCY_005057"
     CDS             2251..>2633
                     /locus_tag="ACHLCY_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0372107.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSIT"
BASE COUNT          612 a          603 c          825 g          593 t
ORIGIN      
        1 accgaagagg tgatgctgga caaaattacc ggaaaaacgc cggtggcggc ctatgtctcc
       61 ccgatcgttg aaggaaaagt gctgcgccat cgtggtggtg aaacccgcgt gttgcgtccg
      121 ggctacgtca agccgaaaca cgaatttaat taccagcagg cggttgagcg ccttcctggt
      181 gaagatccgg ctcagctgaa cgacccggcc taccgtcgtc tgcgtatcat taccgataac
      241 ctcaaacagg aagagcacgc cattgtccag gtggaagaaa tgcaggcggt gaatgccgtg
      301 ctgtatggca aatacaccat ggaaggggat cagtttgata ctgtcgaggt ggatttcggg
      361 cgctctgaag gaaataacat tgagcaggct gacggtaaaa aatggtctga gcaggaccgt
      421 gatacgtttg atccgacgca tgatattgac ctctactgcg atcaggccag cggccttgtg
      481 aatatcgcca ttatggacgg tacggtctgg cgtctgctga atggctttaa gctgttccgc
      541 gaaaaactgg atacccgtcg cggctcaaat tcacaactcg aaacggcagt gaaagatctg
      601 ggcgcagtgg tgtccttcaa ggggtattac ggcgatctgg ccattgtggt ggcgaaaacg
      661 tcttatgtgg cagaggacgg taccgaaaaa cgttatctgc cggagggcat gctggtgctg
      721 gggaatacgg cggcagaggg gattcgttgc tatggtgcca ttaaggatgc acaggcgttg
      781 tctgaaggag tggtggcttc ttcccgttac ccgaaacact ggctgaccgt gggcgatccg
      841 tcctgtgaat tcaccatgac gcagtccgct ccgctgatgg tgctgccgga tccggatgag
      901 tttgtggtgg tacaggtgaa ataatccgtg agcgggggcg aaatgccccc gtgtcttttt
      961 tcacaggagg ctgagatggc aacaaaagaa gaaaatctga atcgtcttcg tcaactggct
     1021 ggcctgctgg ggcgcgaggc ggatatgtcg gggagtgctg cggatattgc tcaacgtgtg
     1081 tctgagtggg aagaggagct tgctgtttcc ccggagggca ttatgcactc tgatgagagc
     1141 ggggctgatc aaaatcacac agacgatggt gagcagttga acaacacgga tgctccggat
     1201 gatgttaaag ccgtccgggt acggaagtgc ctgcaagtaa tggggtattg cccggagaca
     1261 ggtcgtcccg ttgagctggc gttacggggt atgcgtgttc tggtgccatc atcactggca
     1321 acggccatga tacagcacgg aacggctgaa tatgcgtgat tttcagaatg cctttgatgc
     1381 tgccctcgcc ggggtagaca gtacgatcgt tgaagtgatg gggctctgtg cgcagttcac
     1441 ctcgggggca cagtgtggca gcgaagttca gggggttttt gatgatccgg agtcgctggg
     1501 gtttgccggt ggcggggtcc gtattgaagg aagcagcccg tcattatttg tgcggacgga
     1561 tacggttcgt gccgtgcggc gtggtgacac gctgaccatt aatggtgaga tattctgggt
     1621 ggatcgtgtt tctccggatg acgggggcag ctgttatctc tggctcaacc gtggtcaacc
     1681 acccgcagtt aaccggcgac gataaacgca gggtgaaatt atggcgataa aagggcttga
     1741 tcaggcgatt gacaatctga gccgggttcg taaaaacgcc attccggcgg cttcagcaat
     1801 ggccattaac cgcgtggcca caacggcgat taatcagtct tcatcacagg ttgcccggga
     1861 gacaaaggtt cgccggaaac tggttaagga acggtccaga ctgaaacggg cgacggtcag
     1921 aaatccgaat gccagaatta tcgttaaccg cggtgatctc cctgtgatta agctggggat
     1981 caggatgctg gggcgtcgcc cgaacagcat acttaaagcc ggtcagcatc ggtatcagcg
     2041 ggcatttatt cagcgattaa aaaatggtcg ctggcatgtc atgcagcgtg tggccgggaa
     2101 aaaccgttac cccattgatg tggtgaaaat cccgatggcg gccccactga aacaggcatt
     2161 tgatgagaat gttgaccgta tccggcgtga acgcctgcct aaagaactgg catacgcgct
     2221 gaaacaacaa ctgaggattg caataaaacg atgaaacaca ctgacattcg tgccgcagtg
     2281 ctggatgcac tcgagcagca tgaacacggg gcgacgctgt ttgatggtcg ccccgttgtt
     2341 tttgacgaag aggattttcc tgcgatcgcg gtttatctga cggatgcaga gtataccggt
     2401 gaagagctgg atgcagatac ctggcgggcc acgctgcata ttgaggtgtt tttaccggca
     2461 caggtaccgg attcagagct tgatcagtgg atggaaagcc ggatttaccc ggcgatgacc
     2521 gcgatcccgg cactggcagg actgattacc acgatggtta cgcagggcta tgagtatcgt
     2581 cgtgatgacg atatggcgtt atggagttct gcagatctga cttattccat tac
//
LOCUS       DAZGTR010000276         2625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGTR010000276 DAZGTR010000000
VERSION     DAZGTR010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.278"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..259)
                     /locus_tag="ACHLCY_005058"
     CDS             complement(<1..259)
                     /locus_tag="ACHLCY_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372108.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFG"
     gene            complement(256..1881)
                     /locus_tag="ACHLCY_005059"
     CDS             complement(256..1881)
                     /locus_tag="ACHLCY_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372109.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSKIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNVALATEQIVSPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGGA
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            complement(2176..2364)
                     /locus_tag="ACHLCY_005060"
     CDS             complement(2176..2364)
                     /locus_tag="ACHLCY_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372110.1"
                     /translation="MPENRRIILDSKESWLIICDSQLGHLMRSMYQGRRFIQLNLEKL
                     KGVHDVALPVKWEFTRRQ"
     gene            complement(2504..>2625)
                     /locus_tag="ACHLCY_005061"
     CDS             complement(2504..>2625)
                     /locus_tag="ACHLCY_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372111.1"
                     /translation="RVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          676 a          750 c          550 g          649 t
ORIGIN      
        1 agccaaatcc accggtcgcc ttaaaattca gcggttttgt gggaaaatca acagtcatta
       61 aagtactgtc aatctcaata cccggattca gtgcatatga ggcaccaccc gatgttcgac
      121 gccgggcgag tttaagacca accagttcct cacgggtcac aaatgcgtga cgtccgtcac
      181 cacgctgccc ggtgccaatt tccatattct ccacaacagt ggataaatcc ttccccgcac
      241 gccacggttt tctggtcata ccggcatctc cgacattgat gtactcaggg ttattcgttc
      301 cacctgcccg aatccggata ccatcacctg ccagttttgc ccggttgctg ccggaagtct
      361 caccacactt cccttaaacg tacccggcgc aaaatgaatc acaggaacat catcagccat
      421 aatggtgatc cccacccgct caggcgccgg agatttcacc ctgatacagg aaaaagaggt
      481 tctttcaggt aatgaaaaaa tttttgaatg ccaccttatc gtggagggca gagccccccc
      541 ggcaagcact gacattttgt ctcctgtcac cacgcgcatc atatctttcg cgagatcaac
      601 ccatgcgcag tcaaacggtg tactgagata acggatatcc atgttcaccg gactgaatac
      661 aaacacatcc tgcttaccat ccggtttcgt gtaacaggca atgtattcac cacgccaggg
      721 ataagccaca atggaggccg gattaaactg actctgccac tgttccggtg aaacaatctg
      781 ttccgtcgcc agcgcgacat taccgtttgc atcaacagac accaggccat ttgttcctgc
      841 atacagcaca aaaccctcca tcgcaaccat actccgcctg ctcagacacg cctgcattga
      901 agggattttg gaaccagaaa ttgtggacgg tgatacccca ctgaacaaat aaggctcccc
      961 ctttgtcgcc accaccagtg acgttcccag cggacagata gctacaatat cttctgccgt
     1021 cgtgtgacga ttcacttccg gccatgcata cggcagatac gcttccgaaa acatcacttc
     1081 attaccggca aacccggcgg caataccgtt agccatcagg caaaggcctg tcatattctc
     1141 tggcggcggc aggtaatccc atgtcgccag ggaaggccca aggtttttcg ccggtatttt
     1201 gtccgtgtaa ctgagcacgg atgcatccag ttcagccaca agtaaaaaat ccgcctcccc
     1261 tccacctgat gcagagcgat aaatccggcg acgtttaata ctggcattct gcaatggcac
     1321 cggagccagc gtcagttgta ccgcagttcc cggagtacgg agtgttacct ccagagacgc
     1381 cggacctggc ggaccttctt caccataatc tgagacaaag gtttccgtat aaaaccgggt
     1441 ttcatcatca ttcgggttat cgtcagaaac atcaccgccc tgctgaacag tacagacagg
     1501 agctgtcgtc ggcgcgggga tccccagacg atacgatgat gtcgggtgat tcccgtcccc
     1561 ttttgtggca atagtcgcat ccgtcacttt aggaaaacgc ccgtcagtgt agtaaatacg
     1621 cccgtggggg tcctgagcga tcggactgcg gatcacatcc accacatccg gccatgcaaa
     1681 ccagaaatcg tcacggtaat gaaaaattgt ttttggctta attgtgaatg ttttctcaac
     1741 cccggatatc tgacgttctg gtgtaataac accaaaccgg aaatggcagt cctccgccag
     1801 tacagcggaa tgctcgggca gcatggatgt cacaacgcgc ggcatcatcc cacgcatcgt
     1861 ggtgatatcg atatagggca tagaaattcc tttgactgct tcaggcttaa aaatcagggg
     1921 atttatgatg gaattcgcta aaatcgcgat cggcatcact gaaacaaaat gccttgcatg
     1981 acattctgat gatgactaaa taacttcatt gattattgca attttctttc ggtttggggg
     2041 agcatattgc tccccttttt tcactgcatt atcattgccg ctgtagcaac gagaagcatc
     2101 actccgcaca cgcaagatgt ttcacactca caccatacag tccatttttc gccattcccg
     2161 tatacagaaa gctattcact gtcttcgtgt gaattcccat ttcactggca aggcgacatc
     2221 atgtacccct ttcaattttt ccagattcag ctgaataaaa cggcgtccct gatacatact
     2281 gcgcattaaa tggcccaact gactatcaca gattattaac caggattctt tactatccag
     2341 aataatcctg cgattttccg gcataaaaaa ctcttgaacc agaattacac ggcatccttc
     2401 actccatcgt attaacatgt ttattacttc actctctaat aacccccccc tatacatgac
     2461 aacaaaaacc ggagccgggc tccggttttt gtgaagctgt cggctatttc atcccgccaa
     2521 tattttccca cgtcccgtca gcacgcagga tttgcagcgg tcttaccaca cactgtatct
     2581 gctttttatc tgcatccagt atcaccacct gtgtgattac cctgt
//
LOCUS       DAZGTR010000277         2553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGTR010000277 DAZGTR010000000
VERSION     DAZGTR010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.279"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(359..642)
                     /locus_tag="ACHLCY_005062"
                     /pseudo
     CDS             complement(359..642)
                     /locus_tag="ACHLCY_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
     gene            complement(642..1022)
                     /locus_tag="ACHLCY_005063"
     CDS             complement(642..1022)
                     /locus_tag="ACHLCY_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0372112.1"
                     /translation="MINRTKLEHILEYARQQRHTGQHCKIPPGDMVEIMEIAMRKAGN
                     SPVTTDGWISCSDRMPEKGQNVLISVNFDSDLVEPLICSARYTGSTFRRGQITVAPGN
                     GIDGNGIEQATHWMPLPEPPQEVN"
     gene            complement(1019..1474)
                     /locus_tag="ACHLCY_005064"
     CDS             complement(1019..1474)
                     /locus_tag="ACHLCY_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-6-deoxy-D-glucose-3,5-epimerase"
                     /protein_id="HFP0372113.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALAALE
                     AEPVLYQSCTRPTWNSGVPWTEWKERSREGYENDLRFTDTPDHAGWINKCRKLYTTPP
                     VPVIQADVAQAIENLKQKLVECNRYNYCADAVKGVEDACRAAMLKGDKS"
     gene            complement(1471..1680)
                     /locus_tag="ACHLCY_005065"
     CDS             complement(1471..1680)
                     /locus_tag="ACHLCY_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372114.1"
                     /translation="MAIAASYTMHLYCDCLQCTDGKYKSPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            complement(1682..1870)
                     /locus_tag="ACHLCY_005066"
     CDS             complement(1682..1870)
                     /locus_tag="ACHLCY_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372115.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAEQNRWKNHLRTKGE"
     gene            complement(2022..>2553)
                     /locus_tag="ACHLCY_005067"
     CDS             complement(2022..>2553)
                     /locus_tag="ACHLCY_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0372116.1"
                     /translation="LALLDEQERNQQYIKRRDQENEEIALTVGKLRVELEAAENNLID
                     SECHVAELEEALRDKQALLEASEKRNAKLQSENAYIRNRYKELDLLIGKNILVMQAAI
                     IEWQATGDAKSGLAWIYNTLFGPGELPDESEKDAQAYFNRKYAPIDEKLMALHKWFWE
                     QSEAERAAGIRIKGGE"
BASE COUNT          564 a          682 c          572 g          735 t
ORIGIN      
        1 catgatgacc tcctttgaag ttttagttag caatcaccag ttagtggctg gtgatcgggt
       61 gtcaactaga gccttcaaag atgctccggg catattcccc ttgcgggtat tgtattacgc
      121 ctctccaccc gacctttgta cggatgtgac tatgccaaat tgcaggcata aaaaagccgc
      181 aaagctatcg ggtgcggatg accgctttga agttctagtg cggtcagtat gcgatagctc
      241 tggcggattt gtcaaatcat gtaggcctta tcttgctgta agccgcgcca ttcgggcttt
      301 tccccagatt tgggggaaac tatctgcgaa gtattcacct ttaacggcaa gttgcaggtt
      361 agccacgacc ttcctccttt ggcttgtgaa tttgtatcgt catgtcgctt tgagtggtga
      421 ctacaatgat agaaccaggc tgaaggctgt taagattgaa tgcttcgtaa aacgaatcca
      481 aggccagcgc ttttttattc tttcggttcc accaacgcca tcccttgcta caggctacac
      541 tgacaatcca ctgtccactc ctgtaagcca tataaaccag atgagcaaaa cctgaaggaa
      601 ggcaatccag tcaataatcg tatatttcgc gaaggagtcc atcaattaac ctcctgcggc
      661 ggttctggta gcggcatcca gtgtgtggcc tgttcaatac cattaccatc aataccatta
      721 ccaggcgcaa ccgttatctg cccacgccga aaggtgcttc cggtatagcg tgcggagcat
      781 attagcggct caaccagatc actatcgaaa ttcaccgaaa taagcacgtt ctggcccttt
      841 tcaggcattc gatcactaca gcttatccag ccatccgtag ttaccggaga attgccagcc
      901 ttgcgcatgg caatctccat gatttcaacc atatcccctg gtggaatttt acaatgctga
      961 ccagtatgcc tctgctgtct ggcatattcg aggatgtgct ccagcttggt acgattaatc
     1021 atgatttatc tcccttaagc atggcagcgc ggcaggcatc ctctacgccc ttaactgcat
     1081 ctgcgcagta gttatagcga ttgcattcca ctaacttctg cttgaggttt tcaattgctt
     1141 gcgcgacatc agcctgtatt accggaactg gcggagtggt atatagtttt cggcatttgt
     1201 ttatccaacc ggcatggtca ggcgtgtctg taaagcgcaa atcgttttcg tagccctcac
     1261 gactacgttc tttccattcc gtccacggaa caccgctatt ccaagtgggg cgagtgcagg
     1321 actgatacag aacaggttca gcttccagcg ctgccagagc aatccgtgcc agttcttccg
     1381 cttcttctgc tggcagtaca acgttgctac caggtccgta tgtttcgcgc cactgcttga
     1441 ttgtcagcag tcgctctttg gtaatagtgg tcatttgtta atcctcaaaa ctttatgccc
     1501 gggcgcaaaa gcacgcgttt tgtctttgct tattcgccag ccatccttgc gcgcctcttt
     1561 tgcacagcca gcccatgacg tacctatata ctcaccgaag tctggcgact tatatttgcc
     1621 atctgtacac tggaggcaat cacaatagag atgcatggtg taacttgcag caatagccat
     1681 atcactctcc tttagtgcgc aagtggtttt tccagcggtt ttgctccgcg ctgggctttt
     1741 tgcaaaaacc acaatccatc atcccgtaat atttcatcaa ccccatccgt cggttgctga
     1801 gtctcaccca ctgccagacg ccaggagcgt ttctacgaac taacagaatc tttgctttac
     1861 ggtttttcat cgttttgctc tcctgcatct ctttgctgct cgtcgtgccg ctgcaatacc
     1921 ggtatggcgg cgctttggtg tcgggatgat gttctttgca attagcgcag aagcccaaaa
     1981 acgagtcgga tacggtaaca agccgataca tgccacacgc attactcacc tcctttgatg
     2041 cgaatgccag cggcgcgctc ggcttcactt tgttcccaaa accacttgtg aagcgccata
     2101 agcttttcgt caatcggtgc atatttgcga ttaaagtagg cctgagcatc tttctcagat
     2161 tcgtccggta attcgccagg gccaaacagt gtgttataaa tccatgctag tccgctctta
     2221 gcgtcgccag ttgcctgcca ttcgataatg gcagcctgca tgaccagaat gtttttcccg
     2281 attaataggt ccagttcttt gtaccggttg cggatgtatg cattctcgct ttgtaatttt
     2341 gcgttgcgct tttctgaggc ttcaagtaac gcctgcttat cgcgtagagc ttcttccagt
     2401 tcagcaacat ggcattcact atcaataagg ttgttctctg ctgcttcaag ctcaacacgc
     2461 agcttcccaa ccgtaagcgc aatctcctcg ttctcctggt cgcggcgttt gatgtattgc
     2521 tggtttcttt cctgttcatc cagcagtgcc agc
//
LOCUS       DAZGTR010000278         2521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGTR010000278 DAZGTR010000000
VERSION     DAZGTR010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.280"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            76..282
                     /locus_tag="ACHLCY_005068"
     CDS             76..282
                     /locus_tag="ACHLCY_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0372117.1"
                     /translation="MHIPQNFLPRSRQKLLRSAPGQSFPAVAVMPLAAKVLKQVMVNT
                     GGNKEQVSAKRQNRGSRNSTDDGG"
     gene            307..594
                     /locus_tag="ACHLCY_005069"
     CDS             307..594
                     /locus_tag="ACHLCY_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372118.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            715..1536
                     /locus_tag="ACHLCY_005070"
     CDS             715..1536
                     /locus_tag="ACHLCY_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0372119.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
     gene            complement(1833..2342)
                     /locus_tag="ACHLCY_005071"
                     /pseudo
     CDS             complement(1833..2342)
                     /locus_tag="ACHLCY_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
BASE COUNT          679 a          552 c          603 g          687 t
ORIGIN      
        1 tgccattcac ctgtttaagc ccctgtcgcc gggggcatag acagccacaa ccgtctccgc
       61 cagtccaggg gtgaaatgca catcccgcaa aactttttgc ccagaagcag gcaaaaactt
      121 ttgcggtctg cccccggaca gtcgtttccg gccgtggcag tgatgcccct tgcggcgaag
      181 gttctcaaac aggtgatggt taataccgga gggaacaaag agcaggtctc cgcaaaacgt
      241 caaaaccgcg gctcccgaaa ctcaacagat gatgggggct gaaaaccaga gagaggagaa
      301 agcaggatga gtacccgaaa tatccacgtt aacaccgcgt cgtacacgct tcttgttgcc
      361 gggaagaaaa aaaacaccgg ggaagagtgg gacgtcctgg aattcagcag cctgactgag
      421 ctgaaaaaat atcgcaaaag ccacccggaa aagatggcct tcagttatag ctacgcgctc
      481 agtcagggtg tggataagca gttccgccat atcaacattg cggaagccga tcacttcaaa
      541 cagttcctgc gtcagattaa gcgtgccggc ctcgatatcc gggcgatctg ctgaccggat
      601 gtgatgaata tctctcctga ttgttgcaat cttgttaatg gagatcgggg gggggatgat
      661 attaagcgga aaaatttttt attaaattta ttctttcata aaggagtagc tgttatgcga
      721 ttagcttccc gttttggtcg gtataattcc atccgccgtg aacgtccttt aacggatgat
      781 gaattaatgc agttcgtgcc ttcggtattt tccggtgata aacatgagtc ccggagtgaa
      841 cgttatacgt atattccaac aatcaatatc atcaataagt tacgtgatga aggtttccag
      901 ccattctttg cctgtcagag tcgggttcgt gatttgggac gtcgcgaata cagtaaacat
      961 atgttacgtc ttcgcaggga agggcatatt aacggacagg aagttcctga aattatcctg
     1021 cttaattcac atgatggttc atccagttat cagatgatcc ccggaatttt tcgttttgtc
     1081 tgcacaaatg gcctggtgtg cggaaataat tttggcgaaa tccgcgttcc acataaaggt
     1141 gatattgtcg ggcaggttat cgagggagcg tatgaagtgc tcggtgtctt tgataaggtc
     1201 actgataata tggaggcgat gaaagaaatt catcttaaca gtgacgagca acatttattt
     1261 ggcagagctg cactgatggt caggtatgaa gatgaaaata aaacgccagt gacacctgaa
     1321 cagataatta ctccccgtcg tcgggaagac aaacagaacg atctctggac aacctggcag
     1381 cgggttcagg agaatatgat aaaaggtgga ttatcggggc gaagtgcctc cgggaaaaat
     1441 accaggacaa gagccattac aggtattgat ggtgatataa gaatcaacaa ggcgttatgg
     1501 gtgattgccg aacagttcag aaagtggaag tcatgataat ataagtaaag ccccgaaaaa
     1561 attttcgggg ctttacttat attatcattt ttcggcattg ctgtgtcgtc tgatatacct
     1621 caatttcatc gaccggcttt ccggttgtct gtaaatattg tgcccgcgaa aaatggtctg
     1681 gcaggaggct gcgcagttac cgggtatata taagatgggt tcccgcttcg cggggcttcg
     1741 gccagtcagt attgtcactc atatgatatt tttgtgtgtg gccttccatg ccgcttttgc
     1801 ggcataaccg gtatctgaca atgtctgtaa gattaattat tctgcacgct gttaatttcc
     1861 ggaagtgatt tcttcgtggc cggaagccgg atacctttac tgctttttat cccggtataa
     1921 atccggtaaa cctctgatgc gtaggcaaga cgtctctggt tctggcgttc ggatttcctg
     1981 aatcctgcat tgtatgcacc aacggcctcc caggtgacgc cccatttttt aaatgctatt
     2041 gccagataat aagcaccggt ataaatgttc atgcagggat ctgttgtcag atgttatggc
     2101 ttaattccgt agcgggccag ttcgttaaaa tgctgggaat ctacctgcat cagtccgctg
     2161 ccatatcccg ttaccggatt aataccgatg gcattaaccc ggtaacggga ttctttccat
     2221 gatattgctc tcagtaaatc aggatctatt ttgtaatccc ggcctgcaag atcaaagcaa
     2281 tcagtggcct ggcagatctc atttataaac atcaggcaga tggctaacat ccattttttc
     2341 atttttccac ctctggtgac tttatccgta aataatttaa cccactccac aaaaaggctc
     2401 aacaggttgg tggttctcac caccaaaagc accacacccc acgcaaaaac aagtttttgc
     2461 tgatttttct ttataaatag agatttatga aaaattagtt tctcttactc tctttatgat
     2521 a
//
LOCUS       DAZGTR010000279         2487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGTR010000279 DAZGTR010000000
VERSION     DAZGTR010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.281"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(19..366)
                     /locus_tag="ACHLCY_005072"
     CDS             complement(19..366)
                     /locus_tag="ACHLCY_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0372120.1"
                     /translation="MVTKTITEQRAEIRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIRWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            complement(403..1908)
                     /locus_tag="ACHLCY_005073"
     CDS             complement(403..1908)
                     /locus_tag="ACHLCY_005073"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0372121.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGASSLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAMFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            complement(1898..>2487)
                     /locus_tag="ACHLCY_005074"
     CDS             complement(1898..>2487)
                     /locus_tag="ACHLCY_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0372122.1"
                     /translation="LKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSY
                     SSARASANESWRYFMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSA
                     WSRAEWIGAGRMAIDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAE
                     RQKAGLSRPVWIAQAYEQQIAESRRPEEETTPRET"
BASE COUNT          488 a          811 c          646 g          542 t
ORIGIN      
        1 cggtggcctg ataacgtgtt acggcagcgc cgcgtgactc agggcactgc cggcaaaggc
       61 gttggccttt ttgtgttcat ccacactttc aggccagcgg attgcctccg tcgcaaaggt
      121 ccccgacttg taataggtca gcgccgtctc tgtgccttca agcggcagta ccagtatgcc
      181 aaccgcacta ccggcttttt gtccgtccca gaccaccagt ttcccggtgg cctcatccag
      241 catcaggggc gtcagtgccg gtgttgccga ggaaatcccg ctgctgcctg tggcggtatg
      301 agccggatca ttaccggcaa aaatacgtat ttccgcacgc tgttcagtga tagttttcgt
      361 caccatattg taaaaacctc ctgttgatgg tcagcactgg cttcatggca tggccatgag
      421 cattttcacg tccgcatcac cgtctgctga cgtctgtggc acgccaccct gtaccgctgc
      481 cggtgaatgg ttcgccatga tgcgttcaaa catggcggtt gtggatgcag agaccggttc
      541 tgccttacct gatcccgcag ccagcacagc ccgggcgctc tccacagtca ttcccgggca
      601 ggcagccagc tgttcagcct gcgcctcagc cccttttgcc tcatccagtg ccatgatctg
      661 atcacggagt gagggtccgg catccgcctg cggtgaagca gccaggatcg ggcgggcttt
      721 ttccaccgtc atctccggca tcgccgccag cgttgccgcc agttgttcac gacctttcgc
      781 ttcttcacac gccataatgc gatcggcttc actctgcgcg gatgccaccg gctgctgtgg
      841 tgccgccgcg gccagaatcg cccgggcctg ttcaacgctc atgccctgtt gtcctgccag
      901 catcgtggca agctgttcac gtcctttcgc ttcctggcat gtcaggatcc ccatcactcg
      961 ctggttctcc tgcgcggcgg cttccgttgc agttaattgc ggcatagtgc ctcctctgac
     1021 attactgttc agcgccgtgg ccatcacact gatggcatcc gacgcattga ttaattcatc
     1081 cgccagcccg gcctcaatgc cggactgacc ttcaaaaacg gcggcctctg ttcccgtgac
     1141 agcatcaaca gacagaccgg tatacatcgc tactttttca gcaaacatcc ggcgcgccgc
     1201 atcaatgcgc tgttgcatgt cctggcgaac ctctgccggc aacgcttcaa actgattgcc
     1261 atccaccttg tgcgcccctg agtaaatcag cgtgatatca acaccggcct gcgccagatg
     1321 accggcatag ctgacatggc tcatcatcac gccaatggag ccgatacggg atgtctgggt
     1381 aaccagccgt cgggagcagg ccgacgccag cagcatggct gcagagcagg ccgtgtcatt
     1441 gcacagtgcc cagaccggct tctgctgacg gaggcggtaa atcatgtcag cgcagtcaaa
     1501 cgcgccggcg gcctgcccgc ccggactgtc aatgtccagc agtacgcccc gcacctggct
     1561 atccgccatt gcctgctgaa gacaggcgac aatgccgtca tagcctgtca ttccggaaaa
     1621 tggccgcata ccccccagcc ggtgcaccag cgtgccggtc accggcagta cagcaatacc
     1681 gttcaccacc cggtaaacac gggccggtcg tttacctccg gccatgtact cgtccgtttc
     1741 agccagcatt ccgggagcat caaactgtac ctgctgttgt ggtaccgaaa gacttgatgc
     1801 ccccatctcg cgcccgagcg cgcaaaagaa aacccgcgca taggcgggct ccagaagcag
     1861 cggttcattg aatgctgcgg caataatgtg tgaaagatta cgtctcacgt ggtgttgtct
     1921 cctcttccgg cctgcgactc tccgctatct gctgctcata cgcctgcgct atccacaccg
     1981 gacgtgagag tccggctttt tgtcgctcag cagattccct gacctgctgg cggaaaatgt
     2041 cctgataatc ctcgcccatc agcgccagct ctttctcata cgtgctcagt ccggcctcaa
     2101 tgcgcatcac tgattcctgg acttccttga gcccgtcaat ggccattctt ccggctccaa
     2161 tccactcagc ccgtgaccag gctgatcgcg cctgataaaa atcaaaacgc gcccgtggcg
     2221 gacgaataat cccccgaaga agtgcctctt ccagccagca ggaaaacatc tgcgtggcca
     2281 gccgggacgc aataaatttt cgtcgtccca taaaatagcg ccacgactca ttggcggagg
     2341 cgcgggcact tgaataactg accttcgagt aatcccggga caactgttcg taggaaacgc
     2401 caagaccggc ggcgatatac cgcagcagcg cctgttcaag cgccgaaaat ccattgtctg
     2461 aatcctgcgc agtctgcagt ttcagat
//
LOCUS       DAZGTR010000280         2480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGTR010000280 DAZGTR010000000
VERSION     DAZGTR010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.282"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..374)
                     /locus_tag="ACHLCY_005075"
     CDS             complement(<1..374)
                     /locus_tag="ACHLCY_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0372123.1"
                     /translation="MAFKHYDVVRAASPSDLAKRITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGSISSPEEPGNRPTTSAPSVAPEYYYVIALAGQSNGMSYGEGLPLPDTF
                     DSPDPRIKQLARRSTVTPGGAV"
     gene            complement(905..981)
                     /locus_tag="ACHLCY_005076"
     tRNA            complement(905..981)
                     /locus_tag="ACHLCY_005076"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Arg,seq:tct)
     gene            complement(995..1071)
                     /locus_tag="ACHLCY_005077"
     tRNA            complement(995..1071)
                     /locus_tag="ACHLCY_005077"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1035..1037),aa:Arg,seq:tcg)
     gene            complement(1079..1154)
                     /locus_tag="ACHLCY_005078"
     tRNA            complement(1079..1154)
                     /locus_tag="ACHLCY_005078"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1119..1121),aa:Ile,seq:cat)
     gene            complement(1596..2030)
                     /locus_tag="ACHLCY_005079"
     CDS             complement(1596..2030)
                     /locus_tag="ACHLCY_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0372124.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            complement(2023..2217)
                     /locus_tag="ACHLCY_005080"
     CDS             complement(2023..2217)
                     /locus_tag="ACHLCY_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP0372125.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            complement(2214..>2480)
                     /locus_tag="ACHLCY_005081"
     CDS             complement(2214..>2480)
                     /locus_tag="ACHLCY_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0372126.1"
                     /translation="HYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRI
                     GQEAVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
BASE COUNT          592 a          673 c          566 g          649 t
ORIGIN      
        1 catactgcac cgcccggtgt caccgtactg cgacgcgcta actgtttaat acgtggatca
       61 gggctgtcga atgtatccgg caatggcagt ccctcaccgt atgacatacc attggactga
      121 ccagcaagcg cgatcacata gtaatattct ggcgcaacag aaggcgctga ggtcgtcgga
      181 cggttgcctg gctcctctgg tgatgagatg ctcccctcac tcacaactgg ctggatgaac
      241 tccgcaccat aaccagctgt cgaaatcagc gcactaccat aaggctgcca cccttccttc
      301 agtttttgag ttattcgttt cgcaaggtct gacggcgacg ccgccctgac aacatcatag
      361 tgtttaaatg ccatgaatcc tcccggccgg gataatattg tgagtaaaat aaggagcggg
      421 ctgaagtccg gaagttacag gacaatggca gaagggagac tacagcccgc aattcgaaaa
      481 aggtcgcgca gttgcgcaga gtgattacta tagggtatta ttcgccagct gaaatattac
      541 ttcacgtttc attgtttatt ccttgccgcc cgcgtctccc agcgcgggct tttttgtcca
      601 taagaaagcc cctccggaga ggggctggag agtggcgcta tgtgccattg catggtgccg
      661 ggtgcctccc ggtgagttca gcccggtgcc actaaaccct cgtcattctc gttttgataa
      721 tcagagatta taccgtcacc agtcgcccct ccgctcaggg ggattcacca tgcaaaactt
      781 ttttaacaac tctccgtcaa ccagacaatc atcaactgcc tgaattgtga ggcatttaac
      841 atttcactgt ccagtgtctt tcctgtaata aaaagcccgc aaaagcgagc ctggtaaata
      901 aatatggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct
      961 gtccggctga gctaacaacg cagtgtgcag ataatggacc gccatcgagg actcgaaccc
     1021 cgcgcaacca gcttcgaagg ctgacgctct atcccgatga gctaatggcg gtatgtgatg
     1081 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1141 tgagctaaag ggccgggcgc aggataataa cggtacgtaa ctaattctgc aatatcatcc
     1201 gttctgactg actacattct gaacttccct gaccgtctgc tcaaaacgcc cgctctccag
     1261 ctcaacgcca attgcacgac gccctagcgc cattgctgct ttgacgctac ggacataaaa
     1321 aagccagcca ctgggggagg ctggcaaact cgtagagcaa aatgctgtta cgcaaacttc
     1381 gttacagggt tatcctgcaa tacttaaaat atacaatatt tagaaaacta atagtgctat
     1441 atgcgatttt taagattttg ttattaatta cggtcgcacc ttcctttctg tgtactttcc
     1501 gtatagctca caggattcgg ggtacaaaaa aacccgcgca tcggcgggtt cgactgcgtg
     1561 gcaatgtaac cactcttatc atgatatgca gatttttacg atcgtaaact attttttcgc
     1621 tgataaaata cagaggttct ccctcccggc aattcacgct caacataccg atccatctca
     1681 agcctcactc ccagcatcat cagcatgcct tcaacaatcc cctccgcttt gtgaaggcgt
     1741 ttacctatac aggtgtcaga gcacccatgt ttccgtgcca acgccatgaa cgtctccccc
     1801 aacacgtaat aatcaaccag caagtcatgc agatcgcgat tgttccggta aaggcgggct
     1861 atgcacccgc atatcaccat cgcatcatcg tcacagcact gtggacgtga ttttactttt
     1921 tcggggatca gtcccttaaa tccggcagca atgggcgacc atgtaacatc ctcatagtta
     1981 tttgccgccc atgcccccca gcgctcaaga acctgccgga tatcacgcat cagtatcttt
     2041 accccatccg cggtgaacca taagaacacc gttgacgata gcgtgtcttt tcccttcttt
     2101 atcgccagtg tattttctga ccgtgttgcg actacagttc agtattctgg ctacctcggt
     2161 ctgattttca tatgcctcaa cgagcatgtc aggaatggtt tttactgtga acgtcatgcg
     2221 gcctcacttc tgctgtttcg caggtcttta agtttctgct gatacttcgc cttgatcgcc
     2281 ctgcattctt cgacagtcca gcgatggcgg ttatggtttg attcgatttc gtctactgct
     2341 tcctgcccga tgcggttaat cagttcgacg cgatacggaa cgagatttcc gcttttatgt
     2401 tggttgcaca ccacgcattg cttgtgaata ttgcgttcat caaatcggag ttgaggcgca
     2461 gcagcagttg tccggtaatg
//
LOCUS       DAZGTR010000281         2474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGTR010000281 DAZGTR010000000
VERSION     DAZGTR010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.283"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..196
                     /locus_tag="ACHLCY_005082"
     CDS             <1..196
                     /locus_tag="ACHLCY_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP0372127.1"
                     /translation="LLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKF
                     TPKGVQWIAGLLAAWRATAA"
     gene            291..422
                     /locus_tag="ACHLCY_005083"
     CDS             291..422
                     /locus_tag="ACHLCY_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372128.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVVLFLSITWMALTSAGLVG"
     gene            645..830
                     /locus_tag="ACHLCY_005084"
     CDS             645..830
                     /locus_tag="ACHLCY_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0372129.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPS
                     PDWQTPLNGIISSSENG"
     gene            1231..1557
                     /locus_tag="ACHLCY_005085"
     CDS             1231..1557
                     /locus_tag="ACHLCY_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP0372130.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(1689..1889)
                     /locus_tag="ACHLCY_005086"
     CDS             complement(1689..1889)
                     /locus_tag="ACHLCY_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0372131.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            1931..2296
                     /locus_tag="ACHLCY_005087"
     CDS             1931..2296
                     /locus_tag="ACHLCY_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0372132.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
BASE COUNT          578 a          544 c          689 g          663 t
ORIGIN      
        1 tttgttggag aacggaataa tgtatcgcct cgctggaaaa atgacgcctt actcgcatca
       61 cctggatgcg gggcggttta gcgtgaaaac gggcgaggcg ggcaatggtc atgcttttac
      121 gcaggttaaa ttcaccccaa aaggtgttca gtggattgct ggtctgcttg ctgcatggag
      181 agctaccgca gcatgaagat gataaaaacg gactggaaat ttttgctggt ctggctgatt
      241 ccgtttttat gggtggttgc ccggttaatt actgctatta aggggtaaag atgtcagaca
      301 aactcataat gctggcgaag ggcctttgtg taatcgtcgg tatttcattt tcactaatgc
      361 tggttgttct ttttctttcc ataacctgga tggcgttgac ttcggcaggg ctggtggggt
      421 gagcataaac cgaatgcttt ccgcgtttac cgttattctg ctggtggtct gtggtgcgct
      481 tagtctgggg ctgaatcatt accgcgataa cgtcatcacc tacaaagcgc agcgcgataa
      541 aaaagccaga gagctggagc tggcaaacgc aaccattact gatatgcagg tgcgccagcg
      601 cgatgttgct gcgctcgatg caaaatactc gagggaatta gccgatgcga gagctgaaaa
      661 tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc ctgcggatca acgccacctg
      721 ctccggtacc gtgcgtgaag ccaccggcac ctccggcgtg gataatgcaa ccgtcccccg
      781 actggcagac accgctgaac gggattattt catcctcaga gaacggttga tgacaatgca
      841 gaagcagctg gaaggggcac aggactatat ccgcactcag tgcctgaact aagttttgct
      901 gatgcgccgt atcgtcgctg tattccctca ttaacagaga ccgcagcccg acagggagac
      961 tcctctgcgc gagtgtgcgg ggagaatcaa aaacgatacg caccggggtt taccgcgtta
     1021 acggagcgcg gcgttgtccc ctcatggtcg ctggttcggt gcgatggtgg aagaaaccgg
     1081 acgatgtgtt acctcgcaag acctgttatg tcatgtgtct gatttgtgat ttaagtcgga
     1141 taattgtcgt tgccattaag cagaggattg atggccgaca gggtggcatt gttagaataa
     1201 gacttattct tatctgtgcc gggaatgaaa atgaaaagaa atcttccgtt aattattttg
     1261 ttgtcttctc tggttatggg ctgtacgcaa cataaaacag atatgccccg acagttggtt
     1321 aaggcattac cacaatatcc ggcctatgca gcggcaaatt atataaaggg acgggttgat
     1381 gtgaagtttg atattggtgc tgatggtact gtcacccgaa ttgagtttat ccgttcagag
     1441 ccgcaccatc tgtttgatga gcaggttgta aaggcgatgg caaaatggcg atttgagaag
     1501 gacaggccgc gtaaaggcgt gaagaaaacg tttatcttta gtccttctgc accctgatta
     1561 tttcatcaga aattaattat cactctgttg ttattctgta cattccggct gggtaagtct
     1621 tgttccgccg ggtatgaaga tgaaatattg ttggaggaca gtgggtacct gctcctgtaa
     1681 ccgaacgttc atttctcgtt atttgtcatg ctggccggac gcagatgcgt tgcatctgtt
     1741 gccagccttc tcctgcaggc ttcaataacc cacgctgaaa agttaccgga acctttatgt
     1801 tcaagggcga tattgatctg ttcaatcatg tgattgggga aacggatatt gcgggttgtg
     1861 gttctgcggg tccggttttt cgatgacata tttatttcct ttactgattg ccatatgacg
     1921 gggattttac atggctgagc ttcgtacact ccagagcaga atcaaaacac tgaatacccg
     1981 acgggtgaat attctgaagg gtgaacagcg tcgtgtcagt ggcagtgcac gtgtttccct
     2041 caagcgtcat atctggctca gggatgccgg gcagtgctgt ctctgtggtc gtgtggttga
     2101 cctctgtgac agtgaactcg atcaccgaat tgcacttcag ttcggtggtg gtaatgagga
     2161 gacgaatctc tggacgctct gtaccgaatg ccatcgacaa aagtctgctc gtgaagcggc
     2221 gggtggtatg ccggacccga cgctgccgga ggtgtccgga ggtagtggca gagcggacga
     2281 catcatcgga ctgtaacccg agcggggggg tatcatccgg cgtaaaaaac gatcgctccg
     2341 gacaccgcgc cccctctcac gcagagaaaa aattcccgtt tcagggcagt taacatgtta
     2401 actggctgtc cgggcatttt tgcggttttt atctttatta ttcagtttgt tgtgcgaaaa
     2461 aaatgttaac tggc
//
LOCUS       DAZGTR010000282         2471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGTR010000282 DAZGTR010000000
VERSION     DAZGTR010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.284"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..570
                     /locus_tag="ACHLCY_005088"
     CDS             <1..570
                     /locus_tag="ACHLCY_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HFP0372133.1"
                     /translation="LDDVCGKAGWRNEYRDIPNNGGVECGISIKIDSEWVTKWDAAEN
                     TQVEAVKGGRSGAMKRAAVQWGIGRYLYNLEEGFAQTSLDKKQGWHRAKLKDGTGFYW
                     LPPSLPGWAIPASDNKPSPENTNQKSPSVDCEQILKDFSDYAATETDKKKLIERYQRD
                     WQLMAGNEEAQAKCVQVMNIRVNELKQEA"
     gene            571..1083
                     /gene="ssb"
                     /locus_tag="ACHLCY_005089"
     CDS             571..1083
                     /gene="ssb"
                     /locus_tag="ACHLCY_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0372134.1"
                     /translation="MGRGVNRVIIIGRLGQDPEVRYSPSGTAFANLTVATSEQWRDKK
                     TGEQKEQTEWHRVVMSGKLAEIASEYLRKGSEVYLEGKLRTRKWQDQSGQDRFTTEVI
                     VGVGGAMQMLGGKQGSNEQSSPQRNNGQQQRQQSQQHGNYGEPPMNFDDEIPFAPVTL
                     PFPRHAIHAI"
     gene            1097..1390
                     /locus_tag="ACHLCY_005090"
     CDS             1097..1390
                     /locus_tag="ACHLCY_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage anti-RecBCD protein"
                     /protein_id="HFP0372135.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYARIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            1401..1568
                     /locus_tag="ACHLCY_005091"
     CDS             1401..1568
                     /locus_tag="ACHLCY_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFP0372136.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKTMPSREELLKRNSFPSVNQNKY
                     LNAMWRKGGKQ"
     gene            1565..1999
                     /locus_tag="ACHLCY_005092"
                     /pseudo
     CDS             1565..1999
                     /locus_tag="ACHLCY_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            2166..2344
                     /locus_tag="ACHLCY_005093"
                     /pseudo
     CDS             2166..2344
                     /locus_tag="ACHLCY_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          759 a          534 c          637 g          541 t
ORIGIN      
        1 ctggacgatg tttgcggcaa agcaggatgg cgcaatgaat accgcgatat tcccaacaac
       61 ggcggcgttg aatgcggaat atcaataaag attgattccg aatgggtaac caaatgggat
      121 gctgctgaaa acacgcaggt agaagccgtc aaaggtggtc gttcaggtgc aatgaagcgt
      181 gctgccgttc agtggggaat cggtcggtat ctgtataacc ttgaggaagg tttcgcacaa
      241 acatctctcg ataaaaagca ggggtggcac agggcaaaac tgaaggatgg aacaggattt
      301 tactggctcc ctccatcact tccgggatgg gcaatcccag catcagataa caaaccatca
      361 ccagaaaata ccaaccagaa atctccatcg gttgactgcg aacaaatcct gaaagacttc
      421 agcgattatg cagcgacaga aactgacaag aaaaaactca tcgagcgtta tcagcgtgac
      481 tggcaattaa tggctggcaa cgaggaggcg caggctaaat gcgttcaggt gatgaacatc
      541 agagttaacg aactaaaaca ggaggcataa atgggacgtg gagttaatcg cgtaatcatt
      601 atcggtagat taggccaaga tcctgaggtg cgctattcac catcaggaac ggcatttgca
      661 aaccttacag ttgctacgtc agagcaatgg cgtgataaga aaactggaga gcaaaaggag
      721 cagacggagt ggcaccgcgt ggtaatgagc ggaaaactgg cagaaattgc cagcgaatat
      781 ctgcgaaaag gctctgaggt ttatcttgaa ggtaaattgc ggacaagaaa atggcaggat
      841 caaagcggac aggataggtt cactacagaa gttatcgtgg gcgttggtgg agccatgcaa
      901 atgcttggtg gcaagcaagg aagcaatgaa cagtcttcac ctcagcgaaa taacggccag
      961 caacaaagac agcaatctca gcagcatggg aattacggcg aaccacctat gaacttcgac
     1021 gatgaaatcc cctttgcacc agtaactctc cccttccctc gtcacgctat tcacgcaatt
     1081 taatcaggag aaaatcatgc cagcgcctca gtatggtgcg gatgacccgc gccgctgttc
     1141 cggcaattcc gtatcggagg tgctggataa attcagaaaa aactacgccc ggataatgtc
     1201 gctaccgcag gaaacgaaag aggaaaagga atttcgccac tgtatatggc ttgcagagaa
     1261 agaagagcgc gagcgaattt accagacatc aatccgacca ttccgcaaag ccacatatac
     1321 ccacttccct gaaattgacc cgcgcctgcg taattaccgc tcacgctatg gcgctatcag
     1381 taatgactga ggaatttacc atgagaggac ttgcatacaa tcccggcatt cttccggcag
     1441 aaatgattat tcgccaacgc gtaaagacaa tgccatcgag agaggaattg cttaagagaa
     1501 attcttttcc gtcagtgaat caaaacaaat atctgaatgc gatgtggcgt aaaggaggca
     1561 agcagtgagc gaaattaatt atcaggcact gcgtgaaaag gcagagaaag caactaaagg
     1621 aagctacatc gtagggcata catctgttaa ccagcacggc aatttaacag gagtttttgt
     1681 ttgccaaaaa tggaaaggag aacccggtgg tgtgattgcg gaatgtcatg ttaactgcct
     1741 ggttgaaaca gatgttcagg cttatgcaaa cgctgaattt attgctgcct ttaatccaaa
     1801 tgttgcgctg gcgcttctgg atgaacggga aagaaaccag caatacatca aacgccgcga
     1861 ccaggagaac gaggatattg cgcttacggt tgggaagctg cgcgttgagc ttgaagcagc
     1921 aaaatcaaaa ctcaacgagc agcgcgagta ttacgaggga gttatctctg atgggtgcaa
     1981 gcgtattgct gaactggaag cgcgggaagt tcaattaccg actcgctacg accttcgata
     2041 tggacacccg ataaatgcag atgagcgaca agtcatgata cctaaagaaa atggcagttg
     2101 gctttacctg attgacctag aacacgcatt acgcgtcgct gacattcgca tcaaaggtga
     2161 gtgatatggc gttaacacac cgcgaactct gtcagattgc gtacaagttc cttaagcgca
     2221 acggtttcaa ggtttgtttt catgaccgct ttatagctgt aaccagtacc ggagaacagc
     2281 cagatgctat gggattcaga aattcagcat catgcctgat agaggcgaaa tgttctcgtg
     2341 ctgacttgtt ggcagataga aaaaagcgtt ttcgtaaaaa tccgtctctt ggaatgggcg
     2401 actggcgatt ctttattagt gagccgggaa ttatttcaat tgaggattta ccacctggct
     2461 ggggattact t
//
LOCUS       DAZGTR010000283         2452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGTR010000283 DAZGTR010000000
VERSION     DAZGTR010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.285"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(226..600)
                     /locus_tag="ACHLCY_005094"
     CDS             complement(226..600)
                     /locus_tag="ACHLCY_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372137.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(612..764)
                     /locus_tag="ACHLCY_005095"
     CDS             complement(612..764)
                     /locus_tag="ACHLCY_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0372138.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            complement(1037..1753)
                     /locus_tag="ACHLCY_005096"
     CDS             complement(1037..1753)
                     /locus_tag="ACHLCY_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0372139.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKADDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTEIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1803..2018
                     /locus_tag="ACHLCY_005097"
     CDS             1803..2018
                     /locus_tag="ACHLCY_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0372140.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            2015..2440
                     /locus_tag="ACHLCY_005098"
     CDS             2015..2440
                     /locus_tag="ACHLCY_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0372141.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
BASE COUNT          646 a          541 c          565 g          700 t
ORIGIN      
        1 aggaataacc ccgccatact taccgccgcg ccatttcgcg gattgccaca accggaagcg
       61 cacgatcgaa ttaaatttaa cgacgaccta tacagagaga ctaacttcgc cgggcgcttt
      121 cgtgttatgc cctgactttt cagggatata tccttttcag taaactgtca gtgccggatt
      181 cttatccgtg tccggcgcac gcactctacc tcacctgtga ataaattaat gattaattga
      241 tattttgttg tttgattcaa ctttcccatc ggatgtgtga tgctttaaat cacaggaatt
      301 aatactgctc gctgtaaaat gattttcaag gggagctatt cgaatccctt tctttttcat
      361 taacaagcca aatcctttat taatgatgtc cattaattcc aggaagtatt tttcatgtaa
      421 atcctggtta tcagagagct gcttctcttc gtacagaccg ataaaggcac gacgcacgtt
      481 accggatata gtatcgatgg tttctttttc tacggtactc aggtcaagag tcgccagttg
      541 agagcgaacc acattcgatg ccatttcctg gaatggtact ggtaaatctt taaattccat
      601 cgtcaacctc atcagtcagt gtttctggtt aaccagcgac gcgcgccagc ttcagtttta
      661 aacgttttgc ttctggtata cgtcatcgcg gtaaacgtgc cgtcctggtt gggaaacacg
      721 ccgtacacca gagattcgtt gttgccaaga tcgatagtat ccatgctgac ctcatttccc
      781 cttaacgctg gggtagcgga actaaaaacc tgctgcgctg ttatacaaag tgttcccgcc
      841 gtcatgttca tacgcctcgg gctggctact taccccctga ccactgcttg gtaactcgaa
      901 gtattgcccg gcgttctgtg gggcggggtg ggttggtatg ttgttaaggt aacaagagtt
      961 accttttggg ttaatacaat gttgcaaaag gtacatttga gggcatgaaa aacccgcaat
     1021 gaatgcgggt tctgactcag tctaagtatt gatgtatttg tgaaacttta cctttaatgg
     1081 tgtaaccacc attcagttcg atgggtttgt aaagcggatt cagtgacaac agatagatgt
     1141 ttggtccgtc aatcgcaact ttttttagtg ttacgtttgg cgttccttcc aattggatta
     1201 agattatttt tcccaccagt tctctaatgt tacttgagca tggtgtgatc agcacggtag
     1261 atccgtcggg gatggttggg aggccgttag agtttgtcat cgcatctccc tcaacatgca
     1321 ataaaaaaga gttttcagcg gtttttgtca tgacatcaac ccaattctta ataccaggaa
     1381 tctcggttac tggacaactc atatcccaat aaccagcctg ttcccacgtt aaaacgggca
     1441 accgggcgat gttgtcacta atgtaagggt actgattcag acgcagatca tcggctttat
     1501 cgtgaccgtc ctttccataa agaatccatt caggagattt ggaaagcaat tttgacagta
     1561 gatacaaatt ctcaccgtca ggttttgaag agccattttc ccattttgtt acggatacac
     1621 gagatatgcc gattgctttc gcaacctgct gttgggttaa tccaacgtct tttcgacgat
     1681 tccgaatacg ttcgctgata gtgtttttca tgtaaccaat gttactacca agtgatgttg
     1741 ctatggttga cattgttatg taactattgt tacccttcta ctcgaaataa caggagagtt
     1801 ttatgttcaa agatgatgtt ctgcgctatt tcaaaaaaaa gcgactagta gctgaggctc
     1861 ttggaatttc acatgtggct gttgtgcggt ggaaagcagt tattcccaaa cttcgcgcaa
     1921 tggaactgga tgaaattact aacggtgaat tgaaatacaa cccagaactt tacaagaagc
     1981 aggatagcac ctcaaacgaa ggaaagaatg attcatgaaa atcaagcatg aacacatccg
     2041 catggcgatg aatgtctggg cgcatccgga cggcgaaaaa gtaccggctg cgaaaattac
     2101 caaagcgtat ttcgagctgg gaatgacgtt cccggaactg tatgacgaca gccatccgga
     2161 agccctagcc cgtaataccc agaaaatttt ccgctgggta gagaaagaca cccctgatgc
     2221 agttgaaaaa attcaggcgt tgttaccagc gatcgaaaag gcaatgccac ctttgctggt
     2281 ggccagaatg cgcagccaca gttcagctta ttttcgggag ctggtggaga cgcgggagcg
     2341 actggtgaga gacgctgatg attttgtagc agtggcaatc gccggtttca atcagatgaa
     2401 ccgtggtggc ccggcaggaa atgctgtggc agttcattga ctgacaatag cc
//
LOCUS       DAZGTR010000284         2446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGTR010000284 DAZGTR010000000
VERSION     DAZGTR010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.286"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..557)
                     /locus_tag="ACHLCY_005099"
     CDS             complement(<1..557)
                     /locus_tag="ACHLCY_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0372142.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
     gene            complement(577..837)
                     /locus_tag="ACHLCY_005100"
     CDS             complement(577..837)
                     /locus_tag="ACHLCY_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372143.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTF"
     gene            complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHLCY_005101"
     CDS             complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHLCY_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0372144.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1406..1510)
                     /locus_tag="ACHLCY_005102"
     CDS             complement(1406..1510)
                     /locus_tag="ACHLCY_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372145.1"
                     /translation="MSDDISLAMEGALAVVAVVGVYCLVVFLMDRLGN"
     gene            complement(1626..2237)
                     /locus_tag="ACHLCY_005103"
     CDS             complement(1626..2237)
                     /locus_tag="ACHLCY_005103"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0372146.1"
                     /translation="MYWQDAPVEGSSKAAAYATGWNDCREAMFQSGNFRENKDSSTNN
                     FRKIPEASTSSPVTPALLPGGFTIEEAKELHEDLARSHISKALSGEKMKKKDRDADLR
                     WIHGVIVQAAWFVKASLEQNALSGNYPVTPDSWISCSERMPDTKTAVLVARDFGRKGD
                     WRMKWATYIPGHPDANDGWIIPGASWIPSHWMPLPEPPQEVRQ"
     gene            complement(2370..2446)
                     /locus_tag="ACHLCY_005104"
                     /pseudo
     CDS             complement(2370..2446)
                     /locus_tag="ACHLCY_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          572 a          678 c          591 g          605 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acagtttcgc tgtgatgata atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcatggatt cacggcctgg cttaacgata accagaccga gttccggtac cagaacaggt
      541 cgaagtaata cccgcacatt acctccagat ccgttgctag aatgtgcggg acggacgcgg
      601 tggccgttcg gagtaaggga gcctgacgga gattatccag tgacggtagt cgaggctaag
      661 ggctttttta acctcgcatc cgcgcctgcg gtaacactga atgagccatt cggcctgttc
      721 ttcagtgcat gggggatgct ggtaccagtc agatttgaat gcgtgaaaac accgtccgcg
      781 cctgctggca aagacggcag aatcatcaga attgtataat ttggtatcgt gcgccatcgg
      841 ttgtctctgc tggcgcagca ggtgccagtt gttcaggctg gcgtgcgaat tgtaaaccag
      901 aatgccagga aaaaacaaaa cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc
      961 tgattcatca gaggtggcga gggatttctc ccccgccggg tctcttactc ctcaggttcg
     1021 taagctgtga agacagcgac ctccgtctgg ccggttcgga ctcgtacctc gcagaggtct
     1081 ttcctcgtta ccagtgccgt cactatgacg gttaaacaga tgacgatcag ggcgattaac
     1141 atcgcctttt gctgcttcat agcctgcttc tccttgcctt tcggcacgta agaggctaac
     1201 ctacgtttgt gaagcataga ttgggcctca gattaatgtt aagcgtcttg caggacgcgt
     1261 aatgttaact ggggcttttc tctgtctgcc ttacggtggc atgcccgagg cagacagcct
     1321 caagcacccg cagcaattct acttaactct cgctttaccg caaaccgttt ttacccgata
     1381 tgggaattcc catatcgtaa tgaattcagt tccctagtcg atccatcaaa aacacaacca
     1441 ggcagtaaac gcccacaaca gcaacaacag ccagcgcacc ttccattgcc agtgatatat
     1501 catccgacat attccctcct ttggtgttaa tcccggcgaa cgtttttacc ctcaccgaca
     1561 aataacatat actaaaaaag cgatagccat agcaacgcct gtaattgcaa atgcttcagg
     1621 ccagttcatt ggcgcacctc ctgcggcggt tctggtagag gcatccagtg tgatggtatc
     1681 cacgacgcac caggtattat ccacccatca ttagcgtcag gatgccccgg gatgtaagtc
     1741 gcccatttca ttcgccagtc acctttcctg ccaaaatccc tggcaacaag aacggctgtt
     1801 ttggtatccg gcattcgctc actacagctt atccaactat ccggagttac cggatagttg
     1861 cccgatagtg cattctgctc cagtgatgct tttacaaacc acgctgcctg aactataacg
     1921 ccatgaatcc agcgcaaatc agcatcgcga tctttctttt tcatcttttc gccacttaag
     1981 gccttgctta tgtggctgcg tgccaggtct tcatgtaatt ccttcgcctc ctcaatggtg
     2041 aaaccaccag gcagaagagc cggagttacc ggagagctgg ttgacgcttc cgggattttc
     2101 cgaaaattat tggttgacga atctttattt tcccgaaagt ttccggactg aaacatggct
     2161 tcgcggcaat cgttccagcc tgtagcgtat gcagccgctt tgctgctgcc ttcaactggc
     2221 gcatcctgcc aatacatttc ttccggcact atcggcgctg gaggggcggc aaatagatat
     2281 ccgccaaagt caggaagctc tctaatggcc tgtacaaatt tttgtttgcc tacgtcaacc
     2341 cctaatgggt aatgagctat aatctttgcc accggctctg cttccagcga tgccagcgca
     2401 atccgtgcca gttccatttg ttcaccacgg gtaagcccgt tttcaa
//
LOCUS       DAZGTR010000285         2403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGTR010000285 DAZGTR010000000
VERSION     DAZGTR010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.287"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..155)
                     /locus_tag="ACHLCY_005105"
     CDS             complement(<1..155)
                     /locus_tag="ACHLCY_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFP0372147.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVH"
     gene            complement(148..294)
                     /locus_tag="ACHLCY_005106"
     CDS             complement(148..294)
                     /locus_tag="ACHLCY_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFP0372148.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            complement(327..623)
                     /locus_tag="ACHLCY_005107"
     CDS             complement(327..623)
                     /locus_tag="ACHLCY_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP0372149.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPAATEDFKQIDMQF"
     gene            complement(765..980)
                     /locus_tag="ACHLCY_005108"
     CDS             complement(765..980)
                     /locus_tag="ACHLCY_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0372150.1"
                     /translation="MSNLRKYRESLNISQTTLAKAVGCTQGAIGHWESGRRFPDLKTC
                     RALVACLNKLGAKVSLDDVFPPEHKAA"
     gene            1056..1751
                     /locus_tag="ACHLCY_005109"
     CDS             1056..1751
                     /locus_tag="ACHLCY_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP0372151.1"
                     /translation="MDDFSTERNNKGMKMKWYELARSRMKELGITQEKLAEELGMTQG
                     GIGHWLRGSRHPSLDEIGVVFKYLGIDNVSFNHDGTFSPVGEYSSAPVKKQYEYPVFS
                     HVQAGMFSPELRTFTKGDAERWVSTTKKASDCAFWLEVEGNSMTAPTGSKPSFPDGML
                     ILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNDSCSVVG
                     KVIASQWPEETFS"
     gene            1897..2119
                     /locus_tag="ACHLCY_005110"
                     /pseudo
     CDS             1897..2119
                     /locus_tag="ACHLCY_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2097..2369
                     /locus_tag="ACHLCY_005111"
     CDS             2097..2369
                     /locus_tag="ACHLCY_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFP0372152.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQEAISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
BASE COUNT          666 a          542 c          552 g          643 t
ORIGIN      
        1 aagtgaaccc acagatgcgc tgcttctgcg tccttgtaaa acggcacatc cataatttta
       61 cggtgcagca aggcataccc cttaccgctg ctttgatgcg gttgttgtag ccttctggcc
      121 tctctggctt cggctagatt agatatgtta ctcatgacct ttctccttct gcatcagctt
      181 cactttttcc aactcagccc ggaatcgacc aggctgcttg aagctggaca ggaagcgatc
      241 acgtagtatg tgtttgtgaa ttttgtcctg gtaaggactg agttgttttg tcataattac
      301 tcctgtggat tgatccagta attccctcag aattgcatat caatttgctt aaaatcctcg
      361 gtggcagccg gggatttttt ctttgtgatt tcatcaagcg catacttaaa agccctgcta
      421 atcggactga tgtctgatgc cattccgaaa gcacacaaga ccgaagcaat aaatctccag
      481 tccgttctgc ttatcttcga ttcatgacag ccaatcatct ttgccagacc gcgctgggta
      541 agcgttgaca ggttgatgag taaatctgtt tctgcgcgat caacgtcgcg ctgtgatagt
      601 ttgctgtaac ttgtttgttc catttcttaa gatttccagt agtgaatagt tagttgaaag
      661 gtatgcgtgg aaacgcatat ggccttagtt ggtcagatat cttggaactc gcttttcagc
      721 gacgtaggac gaatgtccgt tgttacaaag agcggatccg cttattaagc ggctttgtgt
      781 tccggcggga acacgtcatc aagactgact tttgcgccta acttgtttag gcacgcaaca
      841 agagcacggc atgttttaag gtctgggaag cgacgaccag actcccaatg tccgatagct
      901 ccctgtgtgc atccaactgc cttagcaagt gttgtttgag agatattcag tgactctcga
      961 tattttcgta ggttgctcat atgccctcca tagtaaccat aacgcaataa tacgatatgt
     1021 acttttagaa tgcaaacaaa aaatacatct tgtgcatgga tgattttagt acagagcgta
     1081 ataataaggg tatgaaaatg aaatggtatg aactggctag atccagaatg aaagagctcg
     1141 gcataactca agagaagtta gccgaagagc ttggtatgac gcagggtgga attggtcact
     1201 ggttgcgcgg atctcgtcat ccatctcttg acgagattgg tgtggtgttt aaataccttg
     1261 gtattgataa cgtctcattc aaccacgacg gtacattttc acctgttggc gaatactcat
     1321 ctgcccccgt taaaaaacaa tatgagtacc ctgttttttc tcatgttcag gccggtatgt
     1381 tctcgcctga gcttagaacc tttaccaaag gtgatgcgga gagatgggtc agcacaacca
     1441 aaaaagccag tgattgtgcg ttttggcttg aagttgaagg taattccatg accgcgccaa
     1501 caggatccaa gccaagcttt cctgacggga tgttaattct cgttgaccct gagcaggctg
     1561 ttgagccagg tgatttctgc atagccagac ttggtggtga cgagtttacc ttcaagaaac
     1621 tgatcaggga tagcggtcag gtgttcctac aaccactaaa cccgcaatat ccaatgattc
     1681 catgcaatga tagctgttcc gtagtaggga aagttatcgc cagccagtgg cctgaagaga
     1741 catttagtta acagcctcac cactctaaaa cacacaacaa taacccgacc ttagcgtcgg
     1801 gttttctttt tccaaaatat aaacccatta aatacaaagc gttataaaaa actaaaccat
     1861 atttagaaca ttttgtattg actcagtaaa gtacacatcg tactatttag ctatcagcag
     1921 gaagctggaa gccaaacgga acagactggc aggctcttta aacaacgtcg aacactcgac
     1981 tacgtggctg aaaagccaga tcacccaacc acataagctg tgggatgcaa tgccgaagca
     2041 accgtctcag gaggggcttc gagattgcat cgccaaagtt tattcgggag gaatctatgt
     2101 ccagaaaaac agaatttaaa ggaaccgcag cttctcgccg tagagctcgt cgtgcaaatc
     2161 tgcaaagtca ggaggcgatc agctccgaca agctacacag gccaacccct tcacgagtgg
     2221 tcttgcaatg caagcgcaaa ccagcaatga gagcagaagt gataactctg acaacgttga
     2281 ccagaaaata tgaaggttca acttgtcttc cgaacgtagc catttacgcg gcaggctacc
     2341 ggaaatcaaa acaactgacg gcgagatgac ttgtgttggt cgccagaaaa tgaaattagg
     2401 cag
//
LOCUS       DAZGTR010000286         2399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGTR010000286 DAZGTR010000000
VERSION     DAZGTR010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.288"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(90..1502)
                     /gene="pykF"
                     /locus_tag="ACHLCY_005112"
     CDS             complement(90..1502)
                     /gene="pykF"
                     /locus_tag="ACHLCY_005112"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP0372153.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1510..1704)
                     /locus_tag="ACHLCY_005113"
     CDS             complement(1510..1704)
                     /locus_tag="ACHLCY_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372154.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            2059..2268
                     /gene="fumD"
                     /locus_tag="ACHLCY_005114"
     CDS             2059..2268
                     /gene="fumD"
                     /locus_tag="ACHLCY_005114"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP0372155.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          637 a          515 c          576 g          671 t
ORIGIN      
        1 ttgatttttt attgcttctg gttatcgatt aaataaaaaa agcgcccatc agggcgcttc
       61 gatatacaaa ttaattcaca aaagcaatat tacaggacgt gaacagatgc ggtgttagta
      121 gtgccgctcg gtaccagtgc accagaaacc ataactacaa cgtcaccttt gtgtgccaga
      181 ccgctctgca gagccagttc tttacccaga cggtagaaat catcagtaga agtgatctct
      241 ttaaccagct gcggcacaac gcctttgctc agtaccaact gatgagccgt tttttcgttg
      301 gtggtcagtg ccaggatggt ggcatccggg aagtatttac gtactgcgcg agcagattta
      361 ccgccctgag tagcaaccac gatcagcgga gcatccagtt tttcagcagt ttcaacggca
      421 ccacggcata ccgcttcggt aatgcgcagt ttacggttgt cattgttgaa ctcgagacgg
      481 ctgttcatca cgcggtcggt acgttcgcaa atggtcgcca tgatagaaac agcttccagc
      541 gggtatttac cttttgcgga ttcaccagac agcatcactg catcagtacc gtcgaggatg
      601 gcgtttgcaa cgtcaccggc ttctgcgcga gtcgggcgtg ggtttttgat catggaatcc
      661 agcatctggg tcgcagtgat aacgacttta cgtgcacgga tacatttttc gatcatcatc
      721 ttctgggcga agataacttc ttctaccggg atttctacac ccaggtcgcc acgcgcaacc
      781 atgatgccgt cagaggcttc gaggatttcg tcgaagttgt tgaggccttc ctggttttcg
      841 attttggaga tgatgtggat gttttcgccg ccgtgcgctt tcaggtgctc acggatttcg
      901 ataacgtcag aacgcttacg aataaaggaa gcagcaacaa agtctacgcc ttgttcgcaa
      961 ccaaagatca ggtcctgttt gtctttttca gccagtgctg gcagagcaat ggaaacgcca
     1021 ggcaggttca cacctttgtt ttcgcccagg tcaccgttgt tcagcacttt acagataact
     1081 ttgttacctt caatggcggt aacttccata ccgatcagac catcgtcaac cagtacggtg
     1141 ttgccaactg acaggtcagt agtgaaacct tcatacgtta ccgcaaccat ttcgctgttg
     1201 ccgataacag atttatcagt ggtgaaagta aaggtctgac cagctttcag agaaacgtcg
     1261 ttaccgcctt ccagtttcat ggtgcggatt tccggacctt tggtatcaag caggatagcg
     1321 gcggttttac cagttttgct catcacgttg cgcagattct gaatgcgctg accgtgttct
     1381 gcatagtcac catgagagaa gttcagacgc ataacgttca tgccagcgtc cagcatttta
     1441 gctaacatct cttcagattc ggttttcggt ccgatggtgc aaacaatttt ggtctttttc
     1501 atgacagtct tagtctttaa gttgagaagg atgggagaaa cttgctttct gggcgcactg
     1561 ccggaaagcg aatccggtat tacgaaagtg gtggtgcctc gctctaagga taggtgacat
     1621 cgaaaaagcg tgcagaggaa tgtgtgcttg tgtctcagcc caacgtgagt gcctgttttt
     1681 gagtcgttga gttctacagt ccaaagtgct gaaaccattc aagagtcaat tggcgcgcat
     1741 tatacgctga aattaatcca aaaggaaaac aaaaacagcg tttcaaaaaa tatacaactt
     1801 gttagacaat aaattgttat caaagattta acgttccagg gaaaaggtta cgcaaaaact
     1861 tcgctgtttg cgcaacgtct ggtgccaaag ttgtcagtgt gaggataagt gaaagttcat
     1921 ccggtgtata aatatatcat cgctcaattg ctatagttaa aataatttat cgcgttattt
     1981 gtctgatttt gtttgatttt tgtttaattg gttagattga tatatgctga tagttggcat
     2041 gataaatagg tataaagaat gggaaatcgg acaaaagagg atgaactgta ccgagaaatg
     2101 tgcagagttg tcggtaaagt tgtgctggaa atgcgcgatc tggggcagga accaaagcat
     2161 attgttatcg caggcgtatt acgtacagca ttagccaata aacgcattca gcgcagtgag
     2221 ctggaaaagc aggcgatgga aaccgtcatt aacgcactgg taaaatgacc attcaggatg
     2281 aggcggtaag cttcatcctg atattcttgc ttcagttgcg gtccagttaa tcattctaaa
     2341 taacaactgc tgaaattata cgaatcgcga tatgtatttt aatttataac gataatatt
//
LOCUS       DAZGTR010000287         2398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGTR010000287 DAZGTR010000000
VERSION     DAZGTR010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.289"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..249
                     /locus_tag="ACHLCY_005115"
     CDS             <1..249
                     /locus_tag="ACHLCY_005115"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0372156.1"
                     /translation="MLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGLGVRRAA"
     gene            246..428
                     /locus_tag="ACHLCY_005116"
     CDS             246..428
                     /locus_tag="ACHLCY_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0372157.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            703..1407
                     /locus_tag="ACHLCY_005117"
     CDS             703..1407
                     /locus_tag="ACHLCY_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0372158.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGAEVKNIQNNYARN
                     AERFIEGKHFFKVAGDALKNLRVALNYSQNLQPSLRGLQISPKARSLILWTERGAARH
                     AKMLETDQAWEVFEKLEDCYFSQCKKNTGKQEKSTNELSAKEANSLVWLWDYANRSQA
                     LFRELYPALKQIQSNYSGRCYDYGHEFSYVIGMARDVLINHTRDVDINEPDGPTNLSA
                     WVRLKNKELPPSLHRY"
     gene            1482..2205
                     /locus_tag="ACHLCY_005118"
                     /pseudo
     CDS             1482..2205
                     /locus_tag="ACHLCY_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
     gene            2205..>2398
                     /locus_tag="ACHLCY_005119"
     CDS             2205..>2398
                     /locus_tag="ACHLCY_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0372159.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTKLALERRNKERE
                     KAEKTAEKKRRREEQKQKDK"
BASE COUNT          717 a          537 c          605 g          539 t
ORIGIN      
        1 atgcttgtgc cagaggatat gtctgtcgga tggttcagca aggctctgga gagtgttgac
       61 gaagttcgca tcatcactga tggacggatt aattttatcg aaccatcgac agggctggag
      121 aagaagggaa acagcaaagg ctcaatgttg ctgatttggc gaccgttcat cagtcctcga
      181 cggatgttta ctaccgtatc caaagcggca ttgatggcga tcgggctggg cgtcaggagg
      241 gctgcatgag gcgacagcga cgaagtatca ccgacatcat ctgcgaaaac tgcaaatacc
      301 ttccaacgaa acgctccaga aataaacgca agccaatccc aaaagaatct gacgtaaaaa
      361 ccttcaatta cacggctcac ctgtgggata tccggtggct aagacatcgt gcgaggaaaa
      421 caaggtgatt gacgtgatga tttattcggg gctatattcc tcacgcgcca gcaaaatctg
      481 gcgtcgggat tggcgtcctg gatagagacc gtgacagata cacgccgcga gcgtgttttt
      541 tattgtcgta tgcacgcgca catctgaatt atggtgggct gtgtgggggc ggagagatcc
      601 gcgccggtcg gtttcccggt tacgccaacc ctgcacagtt caccaccaga agattggcgt
      661 cgtcggtggt gagttattaa gaaaccactc gagggcgtca ttatgacaac tcaaatctct
      721 gttgaaactc tctccccgat cacccataac cagattcctg ttattaccac cgaacttttg
      781 gcgcagttgt acggtgctga agttaagaac attcaaaata actacgccag aaatgctgag
      841 cgctttattg aaggaaagca ttttttcaaa gtggctggcg atgccctgaa aaatttgcgg
      901 gtagctttaa actactcaca aaatttgcaa ccatctttaa gagggttaca aatttccccg
      961 aaagcccgct ccctcatcct ctggacagaa cgaggcgcag cccgtcatgc aaaaatgctc
     1021 gaaaccgatc aggcgtggga ggtgttcgaa aaactggaag actgctattt cagccagtgc
     1081 aagaaaaata ctggcaaaca agagaagagc accaacgagc tttccgcaaa agaagcaaac
     1141 agccttgtat ggttatggga ttatgctaac cgctcacagg cattattccg cgaactgtat
     1201 ccggcgctaa aacaaattca atcgaactat tccggcagat gctacgacta cggtcatgag
     1261 ttttcgtatg ttatcggaat ggcgagagat gttttaatca accacacacg agatgttgat
     1321 attaatgagc cagacggacc aacgaacctt tccgcatggg taaggcttaa gaacaaagag
     1381 ttgccgcctt cactgcatcg ctactgacaa ttgacaactt aacaaaccca gcttcggctg
     1441 ggttttttat tactgaattt tcaatgtgag aggacatgac aatgaatgag ctgataaata
     1501 gcagtatcat caaaatgaca agcattgaaa tcgctgagtt agtgggaagt cgtccagata
     1561 acgtaaaaat atcaatagag cgcctggcta aatctggagt tattcaactt cctgcattgc
     1621 aggttttcga aaaaaatcaa taacttaggg ctgcgccgta gtgtcgaggc ttacgtcttc
     1681 gaaggcgaac aaggtaagcg cgatagcatt attgtcgttg cccagttgtc gccggaattc
     1741 accgctcgcc ttgttgaccg ctggcgagaa ctcgaagggg caaccgcgaa aataccacaa
     1801 accttttctg aggcattgcg ccttgcggcc gaccttgaag accagaaggc tgaactggag
     1861 aaacagcttg ctctcgcagc acctaaagtt gagtttgccg atcgagttgg cgaggccagc
     1921 ggaattttga ttggaaactt tgcaaaggtt gttggtattg gtccaaacaa acggtttgcg
     1981 tggatgcgcg atcacaaaat ccttattgct tcaggtgccc ggcgcaatgt gccaatgcag
     2041 gaatatatgg atcgcggcta tttcacagtg aaagaaacag cggtcaatac aaatcacgga
     2101 atacagatat cgttcaccac aaaaatcacc gggcgtggtc aacagtggct gacaagaaag
     2161 ctgctagata acggaatgct taaagtaaca ggggaggctg cttaatggct aaaccagcgc
     2221 gaaggaaatg caaaatctgt aaggaatggt ttcacccggc attctcaaat cagtggtggt
     2281 gcagcccgga acacggaact aaattagcac tcgaacgacg aaataaagaa cgcgaaaagg
     2341 cggaaaaaac agcagagaag aaacgacgac gagaggagca gaaacagaaa gataaaat
//
LOCUS       DAZGTR010000288         2378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGTR010000288 DAZGTR010000000
VERSION     DAZGTR010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.290"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(15..212)
                     /locus_tag="ACHLCY_005120"
     CDS             complement(15..212)
                     /locus_tag="ACHLCY_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0372160.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(499..1317)
                     /locus_tag="ACHLCY_005121"
     CDS             complement(499..1317)
                     /locus_tag="ACHLCY_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP0372161.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1469..1840)
                     /locus_tag="ACHLCY_005122"
     CDS             complement(1469..1840)
                     /locus_tag="ACHLCY_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0372162.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1830..2201)
                     /locus_tag="ACHLCY_005123"
     CDS             complement(1830..2201)
                     /locus_tag="ACHLCY_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0372163.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(2214..>2378)
                     /locus_tag="ACHLCY_005124"
     CDS             complement(2214..>2378)
                     /locus_tag="ACHLCY_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0372164.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT          790 a          598 c          411 g          579 t
ORIGIN      
        1 aaataaaaaa gccttcagga ctgaaggcgt ctgtaacaac cgcactgata gtctgccaga
       61 cccgccataa caagctgggt cagtattaac tggcagcgtt cgcgtgaaag gtaagtattc
      121 tgcgcaatct ccccgactgt cgccggttcg gtgacgctta attcatcaaa cacaactctg
      181 gcggtttctg tcatatcctg ctgtttcagc atgtcttttt accctttccg gttaacgtga
      241 cacaccaata actcttgccg aaaaagccag caagctgaaa gacctgtatt aataactacc
      301 agcacattta acgcactgcg ctactttgcg ggcacaaaaa acccgctcag aggcggggtc
      361 aagctatgcg gcgaaataac cactcttaac agcatacctg attttttacg tacgtaaatg
      421 ctttgccgtg catatttttc atgcaaatgt cacgtcctgc tatttttcag tcttataaat
      481 ttaaaaccat agaaaaaatc aattatgttt taaaaatgga taggtaaaga ataacaagtg
      541 acacagattc aaaccaaaat ggaaaagggt ggcaacccac aaacgcccac tccacatcca
      601 tgacaatcca tacacaacac cagataacgt ggcaaataat acaagtaaag caccacctga
      661 atagtgataa aaaccaaaca acaaagccgc cacaattaat gcaaccaatg gagacgttac
      721 ttctgatagc cgtgattgaa tataccctct aaataatgat tcctctgcca gagacacaaa
      781 aaacaaatta gccaatataa actctggcaa ccactcagga aaatgaatct ctggctttaa
      841 tccaccaaaa aaaacagcca aaaacaggat aagaggaaca gagagcgaca gagcccccca
      901 cttccacaaa gacacttctg attttacttc ttttttaaac aaagaagatg tacacaggac
      961 taacaaaaat ggcaccagcg ctttatcaaa attaaaatac attgtatagg gagtactttg
     1021 agggccaaca gtaacagaat ttagcactac aggattgtga aaccctggcc atagatggaa
     1081 agataatgct atggctgata aaactatgcc aacttcatat atagatttag cccaggcgtt
     1141 atatttccag ttgaacttca aaataataaa aaaaacgatt gtagcaacaa aaaataacac
     1201 cgaccaatca ataatatcat taagcacagc caggacaaca gacaccgtca acaatgaaaa
     1261 agccactacc ttatgccaac tgagagttga cagtgacagt actaacacaa tccacataag
     1321 cactcctttt atttaatgat gaagattgat tatcaatatt ttcaattcac caggcaacat
     1381 tttatctacc ttccacaata catgaccatc aaaaaataaa catgttaatt ataaacacag
     1441 aaaacataac cctcatcact atatatccct accgcatatc catatctaac cggacattca
     1501 gagccataag cattccttca ataatacctt ccgctttata aagcctttta ccaataagcc
     1561 catcagaaca tctatgctta cgtgcaagag ccataaatgt cattccacct acgtaataat
     1621 ccaccaacaa atcgtgcaaa tactgattat tcttgtttaa tcgtgccata caaccacaaa
     1681 ttatcatggc gtcatcatca gaacactgag gacgtgattt cactttagtc gggattaatc
     1741 ctttaaaacc agcagctatc gagggccatg ttacatcttc atgattattt gctgcccatg
     1801 ccccccatcg ctcaagaacc atctggatat cacgcgccat cgttaccacc tctgatttcg
     1861 taaatcttca cgcccagccg tccacctggc acaggctgac cgcgcacaat attgatttca
     1921 tcaaactgct cgtcatcaat gagcacttcc gcatgcgtca gcgcatccag cggtgctttc
     1981 agaatgttgt ccaggtcacg gcggcgctta tccggtggtt ctgcaataat ttttattgcc
     2041 agccgtccgg acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg
     2101 cgataacgct caccggcttt tgatacaaaa tatgtgctgc cacgacgtcg ccagtaggtg
     2161 ttcaccgtcg gcgggtaagg caaaacaaac tctatacgca tcagtaacct cttttacccg
     2221 agcacgccgg ttgcaaaagc gtgatcaaga aaacgaaaaa ttaaatcaac ctgggaacca
     2281 tgcttttctt cgaacgccag cggatccgca tgaagctcgt tgtgatgctc ccgacacagc
     2341 ggtagcgtga aaatatcgtg ggattttgtt cccattcc
//
LOCUS       DAZGTR010000289         2323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGTR010000289 DAZGTR010000000
VERSION     DAZGTR010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.291"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..866
                     /gene="stxA1"
                     /locus_tag="ACHLCY_005125"
     CDS             <1..866
                     /gene="stxA1"
                     /locus_tag="ACHLCY_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Shiga toxin Stx1 subunit A"
                     /protein_id="HFP0372165.1"
                     /translation="FSTAKTYVDSLNVIRSAIGTPLQTISSGGTSLLMIDSGTGDNLF
                     AVDVRGIDPEEGRFNNLRLIVERNNLYVTGFVNRTNNVFYRFADFSHVTFPGTTAVTL
                     SGDSSYTTLQRVAGISRTGMQINRHSLTTSYLDLMSHSGTSLTQSVARAMLRFVTVTA
                     EALRFRQIQRGFRTTLDDLSGRSYVMTAEDVDLTLNWGRLSSVLPDYHGQDSVRVGRI
                     SFGSINAILGSVALILNCHHHASRVARMASDEFPSMCPADGRVRGITHNKILWDSSTL
                     GAILMRRTISS"
     gene            876..1145
                     /gene="stxB1a"
                     /locus_tag="ACHLCY_005126"
     CDS             876..1145
                     /gene="stxB1a"
                     /locus_tag="ACHLCY_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP0372166.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            1550..1630
                     /locus_tag="ACHLCY_005127"
                     /pseudo
     CDS             1550..1630
                     /locus_tag="ACHLCY_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            1929..2261
                     /locus_tag="ACHLCY_005128"
                     /pseudo
     CDS             1929..2261
                     /locus_tag="ACHLCY_005128"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2239..>2323
                     /locus_tag="ACHLCY_005129"
     CDS             2239..>2323
                     /locus_tag="ACHLCY_005129"
                     /inference="COORDINATES: protein motif:HMM:NF017860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0372167.1"
                     /translation="MKPPAPPAWIMQPAPDWQTPLNGIISSS"
BASE COUNT          623 a          465 c          606 g          629 t
ORIGIN      
        1 acttctcgac tgcaaagacg tatgtagatt cgctgaatgt cattcgctct gcaataggta
       61 ctccattaca gactatttca tcaggaggta cgtctttact gatgattgat agtggcacag
      121 gggataattt gtttgcagtt gatgtcagag ggatagatcc agaggaaggg cggtttaata
      181 atctacggct tattgttgaa cgaaataatt tatatgtgac aggatttgtt aacaggacaa
      241 ataatgtttt ttatcgcttt gctgattttt cacatgttac ctttccaggt acaacagcgg
      301 ttacattgtc tggtgacagt agctatacca cgttacagcg tgttgcaggg atcagtcgta
      361 cggggatgca gataaatcgc cattcgttga ctacttctta tctggattta atgtcgcata
      421 gtggaacctc actgacgcag tctgtggcaa gagcgatgtt acggtttgtt actgtgacag
      481 ctgaagcttt acgttttcgg caaatacaga ggggatttcg tacaacactg gatgatctca
      541 gtgggcgttc ttatgtaatg actgctgaag atgttgatct tacattgaac tggggaaggt
      601 tgagtagtgt cctgcctgat tatcatggac aagactctgt tcgtgtagga agaatttctt
      661 ttggaagcat taatgcaatt ctgggaagcg tggcattaat actgaattgt catcatcatg
      721 catcgcgagt tgccagaatg gcatctgatg agtttccttc tatgtgtccg gcagatggaa
      781 gagtccgtgg gattacgcac aataaaatat tgtgggattc atccactctg ggggcaattc
      841 tgatgcgcag aactattagc agttgagggg gtaaaatgaa aaaaacatta ttaatagctg
      901 catcgctttc atttttttca gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg
      961 tggagtatac aaaatataat gatgacgata cctttacagt taaagtgggt gataaagaat
     1021 tatttaccaa cagatggaat cttcagtctc ttcttctcag tgcgcaaatt acggggatga
     1081 ctgtaaccat taaaactaat gcctgtcata atggaggggg attcagcgaa gttatttttc
     1141 gttgacttag aatagctcag tgaaaatagc aggcggagat tcataaatgt taaatacatc
     1201 tcaattcagt cagttgttgc cggtctgata atagatgtgt tagaaaattt ctgcatggtg
     1261 aatccccctg tgcggagggg cgactggtga acggtatgat ctctttgatg atcgtaagcg
     1321 agaatacgcg ggtttggtgg caccaggccg aactcaccgg gaggcacccg gcaccatgca
     1381 atggcacata gcgccactct ccagcccctc tccggagggg ctttcttatg gacaaaaaaa
     1441 gcccgcgcag ggagacgctg gcggcaagga ataaacaaca aaacgtgaag taatatttca
     1501 gctggcgaat aatatccgac agtaatcact ctgcgcaata gcgcggccta ttcgctcaaa
     1561 caactgttac ggtcaggtat cccgtcgcga ccaggagagt gcgctggcgt gctggggaat
     1621 tgacagataa gcagaatatt ttgctgaaaa atgcggtttg ctcacacgga cggataacac
     1681 gaaatcctgc gaactgacaa aaactaagtg aataaaagta aaaaccccgt ttgttggctg
     1741 caagcggggt tttgtgtttc ctgactctgg aaaagtcaaa ggagaaagtg tgtttgattt
     1801 tagcaaactg attcgggaga ttcgagtgat ggctgaaaaa ttatctacct ggaagttcat
     1861 tcttatctgg ctggtgtttg tgattatggt ctccggttat ttcatcggtc agatacgctg
     1921 gtggtgaaat gaaccgcgta ctgtgtgtgg tcatcattgc cctgctggtg gcctgtggtg
     1981 cgcttagtct ggggctgaat cattaccgtg ataacgccat cacctacaaa gcgcagcgcg
     2041 ataaaaaagc cagagagctg gagctggcaa acgcaaccat tactgatatg caggtgcgcc
     2101 agcgcgatgt tgctgcgctc gatgcaaaat actcaaggga attagctgat gcgagagctg
     2161 aaaatgaaac tctgcgtgct gatgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca
     2221 cctgctccgg taccgtgcgt gaagccaccg gcacctccgg cgtggataat gcaaccggcc
     2281 cccgactggc agacaccgct gaacgggatt atttcatcct cag
//
LOCUS       DAZGTR010000290         2318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGTR010000290 DAZGTR010000000
VERSION     DAZGTR010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.292"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..91
                     /locus_tag="ACHLCY_005130"
     CDS             <1..91
                     /locus_tag="ACHLCY_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372168.1"
                     /translation="KEIPAERILDTPEWKDYRPKYSGSTYKYS"
     gene            152..427
                     /locus_tag="ACHLCY_005131"
     CDS             152..427
                     /locus_tag="ACHLCY_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP0372169.1"
                     /translation="MPFSIKNRFSSSQVHYPEISGPIKDKPASKNCILTSTTCNVDSY
                     TVYQKKACSFDMRPPGAGERTPKLKLSVTEMTWLSKTIETEIHNTKE"
     gene            complement(507..620)
                     /locus_tag="ACHLCY_005132"
                     /pseudo
     CDS             complement(507..620)
                     /locus_tag="ACHLCY_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
     gene            complement(655..873)
                     /locus_tag="ACHLCY_005133"
                     /pseudo
     CDS             complement(655..873)
                     /locus_tag="ACHLCY_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            873..1025
                     /locus_tag="ACHLCY_005134"
                     /pseudo
     CDS             873..1025
                     /locus_tag="ACHLCY_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein GogB"
     gene            complement(1402..>2318)
                     /gene="espN"
                     /locus_tag="ACHLCY_005135"
     CDS             complement(1402..>2318)
                     /gene="espN"
                     /locus_tag="ACHLCY_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspN"
                     /protein_id="HFP0372170.1"
                     /translation="DGNDFIYNEKQSKILEMKQDRGGNRYSAVDLNSSKYGYYEKVGG
                     GFYRYIESFNPISSETPNKIVYKGESVDLTKEPNSELYSGRYSINNKQVNVYFFRDAD
                     GTFYKSEGLHGGGVIRYIDKPYSQLREGDIGYDEDLLDIYDDSPVLEDTLPALSSEIV
                     PTPEHSIKQIYSKIKEGHIELSDSDIILCRGTTGIQAENIVEYKTAGGLPDSNPNVKA
                     PDEYMAQQQVRIGRILPEYTSDLSVADRFSREHYLIVVKVKAKYITRGSVTESGWVID
                     KTAPVEPLAIIDRTFGMKENISMVNASK"
BASE COUNT          757 a          502 c          396 g          663 t
ORIGIN      
        1 caaagagata ccagcagaaa gaatattaga tacaccagaa tggaaagact atcgtccaaa
       61 atactctggt tccacatata aatattctta atgataccaa cttataacgg aatagcataa
      121 aaacactttt catggagcaa aggagaaaac aatgccattt tcaatcaaaa acagattttc
      181 aagttcacaa gtacattacc cggaaatatc cggtcccata aaagacaagc cagcgtcaaa
      241 gaactgcata cttacatcaa caacatgtaa tgtagatagc tatacagtgt accaaaaaaa
      301 agcctgtagt tttgacatgc gcccacccgg cgcaggagaa agaaccccaa aactaaaact
      361 ctcagttact gagatgacat ggctatctaa aactatagaa acagagatac acaacacaaa
      421 agaatagcaa ccacttacca gagaacacaa aaagccacag acccgaaaca cctgactgca
      481 agcaaccacc tccacaggag gtggttttac catgaacttc ctgtgtactg acttgtgttc
      541 ataataatat ttttgtgttt aaactcaata aagtcacaaa aatgattgta atcatgcaat
      601 gtagtaaaat taaaatattt gaccctgtac acgattctgt gtaaatgcct tttctcagaa
      661 gtgaccgtcc aggcggtcac cgaactcgat aataaagcgg ctcattgcca tacgccagtc
      721 ccgcagtggc atcgtccatt tctgtgaagc cgcctggatt gccagccaca cgaccttttt
      781 cactgagtca tccgtcggga acaccttgcg ttttttgatg gcatgccgga ttacgctgtt
      841 cagcgactcg atggcgttgg ttgtgtagat gatggattta tcgtaataaa gacaacagcc
      901 aaataatgaa aaacataaaa ttttatctgc atggcaaaga gataccagca gaaagaatat
      961 tagatacacc agagtggaaa gactaccgtc caaaatactc cggttccaca tataaatatt
     1021 cttaatgata gcaaaaaata tattttcgat ataatcaatg ttatgattaa agagtatttc
     1081 atcagggcag gtaaaaacag agtaaatcag caaaagaagc tgatcttcag cgatactgac
     1141 actaactgac ggtttaagcg gtcgtatgaa gcagcagctt tccgacggac tgccatgcgg
     1201 atcgtttacc ttttgggcta ttccgcccgt catcaagcgg ctcacgagta ctgagtttat
     1261 caggatgata ttgctgacaa tggtaattcg ttgaccgatg tgtacttcac tatatatcgg
     1321 tcaacaccgt actgccgtgc aacatctcca agatgtagca tcgtttttac cagaggaggc
     1381 aggacataga ttgtaaaaaa actatttcga tgcatttacc attgagatat tttccttcat
     1441 accaaaagtt ctatcaatta tcgcaagtgg ttcaacaggt gcggtcttat ctataaccca
     1501 accactctct gtaacacttc ctcgtgtgat atattttgcc tttactttaa caactattag
     1561 ataatgctca cgactaaacc gatcagcaac gctaagatcc gatgtgtatt caggcaatat
     1621 tcttccaata cgtacctgct gttgtgccat atattcatct ggtgctttta catttggatt
     1681 tgaatcagga agccctccag cagttttata ttcaacgata ttttcagctt gaataccggt
     1741 tgtgccgcga cacaatatga tgtctgaatc ggacagttct atgtgcccct ccttaatttt
     1801 cgaataaatt tgtttaatac tatgttctgg agttggtact atttcagaag ataaagcagg
     1861 caacgtgtct tcaagcaccg gagaatcatc gtatatatcc aacaaatcct catcataccc
     1921 aatatctcct tctcttaact gagaatacgg tttatctatg tatctaataa ctcccccacc
     1981 atgaagacct tctgatttat aaaatgtacc atcagcgtca cgaaagaaat aaacattaac
     2041 ctgtttgtta tttatagaat acctacctga atataattcc gaatttggct ccttagttaa
     2101 atctactgat tcccccttgt agactatttt atttggtgtc tctgaagata tggggttaaa
     2161 ggattctata tatctataaa aaccaccgcc aactttctca taatacccat acttagaaga
     2221 gttaagatct actgcactat atctattccc cccacgatcc tgtttcattt ctaatatttt
     2281 agattgtttt tcgttatata taaagtcatt accatcaa
//
LOCUS       DAZGTR010000291         2308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGTR010000291 DAZGTR010000000
VERSION     DAZGTR010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.293"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..446
                     /locus_tag="ACHLCY_005136"
                     /pseudo
     CDS             1..446
                     /locus_tag="ACHLCY_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            457..1200
                     /locus_tag="ACHLCY_005137"
     CDS             457..1200
                     /locus_tag="ACHLCY_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0372171.1"
                     /translation="MTQTESAILVHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFEREDDWWRNGQNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            1197..1775
                     /locus_tag="ACHLCY_005138"
     CDS             1197..1775
                     /locus_tag="ACHLCY_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0372172.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            2016..>2308
                     /locus_tag="ACHLCY_005139"
     CDS             2016..>2308
                     /locus_tag="ACHLCY_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372173.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGSETALGVEVTKA"
BASE COUNT          494 a          572 c          778 g          464 t
ORIGIN      
        1 ttcggtctgg tcaccggaat ggcggaggac ctgcagagcc tggtggggcc acggtggtcc
       61 gccgccgggt gtatgcgcgt tttctggatg cggtgaattt tgtggcgggc aatccggaag
      121 cggacccgga gcaggagctg agcgaccgct gggtggtgga gcagatgtca gagctgacgg
      181 ccatgacggc ctcgtttgtg ctggcaacac cgacggagac ggacggagcg ctgtttcccg
      241 gtcgcattat gctggcgaat acctgtatgt gggattaccg gggagatgaa tgcgggtata
      301 acggtcctgc ggtggcggat gagttcgaca accccaccac ggatatccgt aaggacagat
      361 gcagcaagtg catgcgcggg tgtgagatgc gcggcatggt ggctaatttt ggcggtttcc
      421 tttccatcaa taaactttcg cagtaaatcc tgttttatga cacagactga atcagcgatt
      481 ctggtgcatg cccggcggtg tgcgcctgcg gagtcgtgcg gcttcgtgat aggcaccccg
      541 gagggcgaac ggtaccagcc ctgcgtgaat atctccgcag agccggaggc gtattttcgt
      601 attgcgccgg aagactggct gcaggcagag atgcaggggg agattgtggc gctggttcac
      661 agccaccctg gtggtctgcc ctggctgagc gaggccgacc ggcggctgca gataaagagt
      721 gccctgccct ggtggctggt ctgccggggg gaaattcata aattccgctg tgtgccgcac
      781 ctgaccgggc gtcgttttga gcacggggtg acggactgtt acaccctgtt ccgggatgca
      841 taccatctgg cggggataac gctgccggat tttgagcgtg aggatgactg gtggcgcaac
      901 ggtcagaacc tttacctgga caatatggcg gcgactggtt tttaccgggt gcccctgtcc
      961 tctgcacagg cgggcgatat cctgctgtgc tgctttggcg catcggtggc caatcatgcc
     1021 gccatatact gcggcaacgg tgaactgctt caccatctgc ctgaacaact gagtaaacgg
     1081 gagaggtatt ctgaaaaatg gcaacgacga acgcattctg tctggcgtca ccgccactgg
     1141 cacgcatctg ccttcacggg gatttgcaac gatttggccg ccgcctcagc ctgtacgtga
     1201 acacggcagc ggaagccatc cgtgccctgt cgctgcagat gccgggattc cgccgtcaga
     1261 tgaacgaagg ctggtaccag atacgtattc gcggtgagga cacggcaccg gaggcggtgt
     1321 acgcccgtct tcacgaacct ctgggtgagg gggcggtcat ccacattgtg ccgcgactgg
     1381 ccggggccgg aaagggcgga ctgcagattg tgctgggggc agcagccatc gtgggctctt
     1441 tcttcaccgc cggcgcaacg atggcgttgt ggggcgcagc cctgagtgcc ggagggctga
     1501 ctgccaccac gatgctgttc tcactgggtg ccagcatgat actgggcggt gtggcccaga
     1561 tgctggcacc gaaggcaaaa acaccggagt acagggcgac ggataacggt aaacagaaca
     1621 cgtatttttc gtcactggat aacatgattg cccaggggaa cccgatgccg gtgccttatg
     1681 gtgaaatgct ggttggttca cgacggatat cccaggacat cagcacccgt gatgagggcg
     1741 gagacgggaa agtggtggtt atcgggcggg gatgaaaata aaaaaatccc gcagagttag
     1801 cggagctgcg ggagagaacg atgaagatta acgttatgga gttatttttc aggcatcaaa
     1861 aaagtaatgc agcgtcatta ttgcggctac aggcaattgc cggaaatgtg aagagtttca
     1921 gaaattttat tccgtcatga cacaggcacc ctccggggtg cctgttgttt tctggcataa
     1981 acagattcag acatcagaca ggagaggggg acagagtggg taaagggggc ggcaaggggc
     2041 acacgccggt agaggcaaag gacaatctta agtccacgca gatgatgagc gtgattgacg
     2101 ccattggtga agggccgatt gaaggtccgg tgaaggggct gcagagtatt ctggtgaaca
     2161 aaaccccact gacggacacg gacggcaatc ccgtgataca cggtgtgacc gcggtctggc
     2221 gcgccgggga gcaggagcag acaccgccgg aaggttttga gtcatccggc tctgaaaccg
     2281 cactgggcgt ggaagtgacg aaggcaaa
//
LOCUS       DAZGTR010000292         2301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGTR010000292 DAZGTR010000000
VERSION     DAZGTR010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.294"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..783
                     /locus_tag="ACHLCY_005140"
     CDS             <1..783
                     /locus_tag="ACHLCY_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP0372174.1"
                     /translation="KEDNGNISQTAKSPAERKRAQRERERKREQNGDCHGESRNVTHM
                     SRRVTTDKDTDKDTDQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALK
                     ADSGAVQQVMTARQEQSHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDNEIPPGFRG"
     gene            824..1246
                     /locus_tag="ACHLCY_005141"
     CDS             824..1246
                     /locus_tag="ACHLCY_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0372175.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNTRKKLSRAKLKKPAVVDPDLIWSLPDG
                     EIRRYDRHQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            1243..1539
                     /locus_tag="ACHLCY_005142"
     CDS             1243..1539
                     /locus_tag="ACHLCY_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0372176.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAERLGHAVIYQEVAQ"
     gene            1536..1997
                     /locus_tag="ACHLCY_005143"
     CDS             1536..1997
                     /locus_tag="ACHLCY_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFP0372177.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRR"
     gene            1975..>2301
                     /locus_tag="ACHLCY_005144"
     CDS             1975..>2301
                     /locus_tag="ACHLCY_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372178.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARI"
BASE COUNT          650 a          490 c          690 g          471 t
ORIGIN      
        1 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc cggcagagcg caagagggcg
       61 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcga gtcacgaaat
      121 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
      181 gaagatcaaa acactatggt ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt
      241 cagaccgtca atcctggtca gccagcaggc acgacaccgg aagccgattc agcgtatgcg
      301 ctgaaagccg attcgggcgc tgtgcagcag gtgatgaccg caaggcagga gcaatcacac
      361 caacttcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
      421 gaaaacaccg gtcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
      481 cgggagtacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
      541 gccagattac gcgagggggt gccaccagag gccatgctgg atggcgtgag gcgttacgca
      601 agatacttgg cggctaccgg gaaaacggga acggaatttg ttcagcgagc gacgacgttt
      661 tttggaccgg accggaattt tgagaacccc tggttgctcc cggtaagcgg cacgaacaac
      721 cagcgttgtg tgaatcatat ttctgaaccg gataacgaaa ttccgccggg cttcaggggg
      781 taagtgttaa tttctggtca tgaggtaatt ttcaggaggg cttgtggcaa aagtttttac
      841 acaagaagag cgggaaaaaa ttaaagggca ggttcttgaa ctcgtacgcc agagtgggcg
      901 cgagacgtta cgacaactgg aagctaaaac tggggcaaca agatatctga tgagcgttct
      961 ggccagagag ctggttgcca gtggcgatgt atacaactct ggttacgggt tattcccgtc
     1021 tgaacaggcg cgtaaggact ggcaaaatac ccgtaaaaag ctctcaaggg caaagctgaa
     1081 gaaacctgca gtggttgatc cggaccttat ctggtcgtta ccagacggcg aaatacgccg
     1141 ctacgacagg caccagaaca taatttgtcg tgagagcagg aggagcgaag ttatgcagcg
     1201 tgtgctggcg ttctatcggg gaaactttca ggaggtgatg gagtgagggt gagagtttat
     1261 attgccggtc caatgacggg atatgaaaat ttcaaccgtg aggcatttca caaggtggaa
     1321 gaggaactga aacgggaagg gcataccgtc ttaaacccgg cagtacttcc ggacgggctg
     1381 acacagccgc actacatgga tatttgcatg gcaatgattc gttgtgtgga tgcgatttac
     1441 atgctgaatg gctggcagcg gtcagcgggc gctaaggcag agctggcact ggcggagaga
     1501 ctggggcatg cggtgattta tcaggaggtg gctcaatgag agaggttaac tatgaggcgc
     1561 ttcgtgaggc agcacaaaac tatcagtcga cgctggcgtg gtatcaggct accccggaca
     1621 gcccaaatgc tgaacgggat tgtgatgcgg ctcttgctgc gtttaagcgt catatccgtc
     1681 atcgggaagc ggatattatc gctggtttgc tggatggact ggaagaagca aaatcacaac
     1741 tcaacgagca gcgtgagtat tacgaaggcg ttatctctga tgggagcaag cgtattgctg
     1801 aactggatgc gcgggaagtt caattaccga ctcgctacga ccttcgatat ggacacccga
     1861 taaatgcaga tgagcgacat gtcatgatac ctaaagaaaa tggcagttgg ctttacctga
     1921 ttgacctaga acacgcatta cgcgtcgctg gcattcgcat caaaggagag gagcatggaa
     1981 ataaaaccag aagatgagtt aagcaatatc gttttatttc cggtaaaaga ggatgaccct
     2041 cgtaatcagg ttaattttct ttatgagcca tcggaaagac catattgtca tcacgcctct
     2101 gtccgggttg acgaaaaaga gcgtcaggtc cgctgtaaaa tctgcggtgc agttgtggag
     2161 ccatttgact ggatgctctc tgtggcgaaa agagaaacta ggctggcaga tgatgtaaag
     2221 ctattgcgcc aggaggaaca ggaaaggcgg aaaaatatag aaaagttaat tcagattgag
     2281 cgtaacgcga aagcgcggat a
//
LOCUS       DAZGTR010000293         2283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGTR010000293 DAZGTR010000000
VERSION     DAZGTR010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.295"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..517)
                     /locus_tag="ACHLCY_005145"
     CDS             complement(<1..517)
                     /locus_tag="ACHLCY_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0372179.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFGAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAVMLEYMKEK
                     DVASMSGRLLNR"
     gene            complement(667..1104)
                     /locus_tag="ACHLCY_005146"
     CDS             complement(667..1104)
                     /locus_tag="ACHLCY_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0372180.1"
                     /translation="MSRVTAIISALVICIILCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGFTADAELNYYRLRGGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1101..1598)
                     /gene="rrrD"
                     /locus_tag="ACHLCY_005147"
     CDS             complement(1101..1598)
                     /gene="rrrD"
                     /locus_tag="ACHLCY_005147"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0372181.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYED
                     IVGVWTVCHGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(1598..1813)
                     /locus_tag="ACHLCY_005148"
     CDS             complement(1598..1813)
                     /locus_tag="ACHLCY_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0372182.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            1956..>2283
                     /locus_tag="ACHLCY_005149"
     CDS             1956..>2283
                     /locus_tag="ACHLCY_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372183.1"
                     /translation="MLHIISPRGISPRQRRLFYPFSSALALFYYAAAFTSGTMLSIST
                     QYVVLLVTNIITINRHRISPVYRNRVKKPEPESNHRMTICQWQVIKKRPRHAQPELTR
                     KMIEGNN"
BASE COUNT          576 a          587 c          500 g          620 t
ORIGIN      
        1 agcgattgag caaacggcct gacatgctgg cgacatcctt ctctttcatg tactccagca
       61 ttacggcgtt acgttctgct tcataacttt cgctgtactt acggagttct gctgacatcc
      121 agttaaacgc attgatatag gcttctttaa cagcatcggc ttttgccccg ttaaatccca
      181 taaccagcat aacgaagcca ctaaagtcca tgcggtagta aatctgtttc ttgtcaaaaa
      241 tccctaagtc attgattttc tcgacggccc caaaatgggt tgtcgagaat tcctcagaac
      301 atctcaaaca tttaatagcc ctaatcacat gctgatggcg tttaccaaat gccctggcaa
      361 tctggaaggt gtcagttacc ggttgacctt ctgctgcggt aactaactgg cgaaagtcga
      421 agtcatgatt cgcaattaat tcattcatgg cgttgcctgc ttctttgaaa tgaacctttg
      481 ccgcacagga aaccagccca ccgaggctcg ccagcactaa ctggtatcct caaaggccca
      541 ttccaaaggg tcaggttcgg tgtttattgt gcgctgcggt gcgcggtgaa ataccggtac
      601 aaaaatgccc cgcatctgcg aggcattttc ctgaaagtca cttgttaaat ttcagtgaaa
      661 ttaaaattat tttaagcact gcgtcctgat gtattcctgc aggtagttaa cctgcgcggt
      721 tatcttgtcg attccacctc ggagacggta ataattgagt tcagcatctg ctgtaaatcc
      781 tgggctttct ccatcgccca tgctgctggc tccggtcgtt gactttgcac aggtggcgga
      841 gacttgcagg cgcttacgac cagcagaaac atcggcacgg agactttcga tagtcgcgtt
      901 agcatcagca agttcctttg tatatcttgc gtcgagttct gctacatcac gttgacgctt
      961 ctgcatatca gcgatgatgg atgtggcttt atcgcgctgc tctttgtagg cgatggcgtt
     1021 atcacggtaa tgattaacag cccatgacag gcagaggatg atgcaaataa ccagagcgga
     1081 gataatcgcg gttaccctgc tcattgctgc ccccacaaac agacttcacg ctcaatctca
     1141 cgacgagtca tcaggccttt ccattgctta ccgccagcgt atgtccagcg acgtagctgg
     1201 tcacatgcgc ctttgatatc gccctggttt attttgcgaa gaagcgtcga tgttctgaaa
     1261 ttaccagcgc ccacgttgta aacgaacgag taaagagcgc cgcgcgttgt ttccggtata
     1321 tcgactttga tgtacgggtt aatttgtctg gcgaccgtgg caaggtcttt attcaggagg
     1381 gctttgcatt ctgcttcggt atacgtttta ccgggaatga tgtcttttcc ggtgtgtccg
     1441 tgacatacag tccatacgcc aacgatatct tcgtatggta tgtagctgac accttccagg
     1501 ccatcgtcac cactcggacc agtgatgagc acagacgcta tggcaacagc cccaccacca
     1561 atagcagcag caacagcctt gcgtaatgat ggcgacatta ttcacctctc gcagccttac
     1621 gcttatcttc tttaatcttg aaataaaggt ttgtcagata cgtcagcaag ccaaacacca
     1681 ggctacccaa tacgcctatt gccacccact gggatgggga gactttgtcc agcaactgca
     1741 gtagccagta tcccgtcccc accgctgacg tggtgtatga cacacctgtt gttatttttt
     1801 ccatctggta cataccccgt ctcccgttat ccggaagctg acaacaataa aaagccacca
     1861 gttaattcct gatggccctg atgcataaac gtcataatac ctgactgtta tgattgacaa
     1921 taatgataat gtttatatag aaaggttccc gatgtgtgtt acatatcatt tctccacggg
     1981 gaatatcccc acgccagcgc agactctttt acccgttctc ttctgcgctg gctctttttt
     2041 attatgctgc tgcatttacc tctggcacca tgctttctat ctcaacacaa tacgtggtac
     2101 ttcttgtaac caatatcata acgattaatc gacatagaat ttctcccgtg tacaggaaca
     2161 gagttaaaaa gccggaaccg gaatcaaatc acaggatgac catctgccag tggcaggtca
     2221 taaaaaaaag gccgcgccat gcgcagccag aactcacaag gaaaatgata gaaggaaata
     2281 aca
//
LOCUS       DAZGTR010000294         2283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGTR010000294 DAZGTR010000000
VERSION     DAZGTR010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.296"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(227..916)
                     /locus_tag="ACHLCY_005150"
     CDS             complement(227..916)
                     /locus_tag="ACHLCY_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0372184.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(931..1053)
                     /locus_tag="ACHLCY_005151"
     CDS             complement(931..1053)
                     /locus_tag="ACHLCY_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0372185.1"
                     /translation="MMSESYISELLRCRWGLVCLCRFPDSVLNDYRMLKNYAKI"
     gene            1394..>2283
                     /locus_tag="ACHLCY_005152"
     CDS             1394..>2283
                     /locus_tag="ACHLCY_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFP0372186.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFN"
BASE COUNT          729 a          509 c          411 g          634 t
ORIGIN      
        1 cgcaccatcc ttcataacca ccccaataca agatgataac attggcttac aactcataac
       61 aaaagcaatt caatgccgtc aagaggttac aggctaaaaa aactctatta catagcagcc
      121 agcatgttta ccgtacaagt acaactcagg gcataaaaaa aacccactcg gcagcgggtt
      181 tatacatttt ttacaacata ccaaatttgc atgaagtata tggcttttaa tccagttttg
      241 caatattttg ctgtaaaaat gctgcctttt gttttgaacg tgttctcgtc acaagcaata
      301 aagcatcact atcaagctgt agaaaaatgt gcttcattgc aacccagcgt tcagtaaatg
      361 tctcggacca gttttttgtt gtcactccca ccaatgatgc cagtgtctgg tattcatagg
      421 cctcacgccc tgcaagttcg ctcttcacat cctgtgcagc cagccagatt aacgtcttca
      481 ggcgatccag tgtcttacct gcaatttttc tgttacttaa caaatcttta aactcgctcc
      541 atgcccattg cgttatggtg acctgatgcc cccatcgaac gctttcgctg taacaccaaa
      601 gcaaccatgc tttctgatgt tcatcgagag acaaaaccgc gcggcgccat gaagaggttg
      661 agaattcaac cgggctgacc aaagcaatgg atgaaccttt tgcgtacgac tgcttaccgg
      721 aagtcggcgt attatccagc gtaatcatct tgccagttac cacatccaga atgcgcggct
      781 tctttcgttt gtatgtacca gtatcaaatt gtgcatgctc ctgccaggct tcgagctggc
      841 ctttcgttgc tccgttcaag tcagcagtag ctgccataag ttgctcacga acatactgta
      901 aatattgggt attcatgcag taaatccttt ctatattttg gcataattct tcaacattcg
      961 gtaatcgttc aaaaccgaat cggggaaacg acataagcac acgagccccc agcgacagcg
     1021 aaggagttct gatatataag actcagacat cattcattcc ccggttctcc aatatctgtt
     1081 tcactcatca tccataacta cctgtaattg cccccccctt ttttttgtaa cagttcttat
     1141 attgctatat agaatagcca ttactaatgc ttttaaattt aataaaataa aaattataaa
     1201 aaacataaaa caccacgcaa acacacttaa taaaaacacc gttacattaa aagataataa
     1261 aaaccgcaat aaaaaacgaa taaatcaatt gtctcacgca attataaaac atcatattga
     1321 ttacgcacct tgtattacaa actcatgtat gtaaaatacg cgcaccattc aaaaaaaagg
     1381 aagacaataa catatgaaaa aaagtgtcat cgctggcgtc tttattgctc tgtcatttac
     1441 cacgtgttca gctatcgcga acagccttgc attatcatta gcaaatgatg atgcagggaa
     1501 gtttcaacca atacttaatg atatttatgg caataaacat gaaaacagag atgattactc
     1561 acaaggctta tttctgggat atagccacga tatctcagac tcgagccaat tatctctcca
     1621 tattgcgcaa gatatttact ctccatcagg cagtaataaa agacacaaca cagctgtaac
     1681 tggagacaga gcttttagtg catacactca cactggtatt gaatggaact cccttgcgaa
     1741 tgactggatt cgctatcgat taggtactga cataggtgtt gttggccccg acgcaggcgg
     1801 tcagaaagta caaaataaag ctcatgagat tattggggca gaaaaatatc atgcatggga
     1861 tgatcaaata gagaatcgct acggttatac tgtaaaaggg atgctatcca tgacaccaag
     1921 tatggatatt ttaggtgcta atgttggatt ataccctgaa gtttctgctg ttactggaaa
     1981 cttatttcaa tatgtagcat atggcgcaac cattgccatt ggtaatgata aaaccttcaa
     2041 ttcggataat ggctttggtc tgctggctcc ccgtggttta atgcatatgt ccgatacaag
     2101 cggattcaaa tacaagattt ttgcaggtat ggaaagacga gatgtcaatc gcaactatac
     2161 tctcgaagga aaaacaatac agacgaaaca aacaacagta tcgctaaaca aaactgttga
     2221 tgaatatcaa gttggcgcaa caattgggta tgcacctgta gccttcacac tagcatttaa
     2281 taa
//
LOCUS       DAZGTR010000295         2274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGTR010000295 DAZGTR010000000
VERSION     DAZGTR010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.297"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..482)
                     /locus_tag="ACHLCY_005153"
     CDS             complement(<1..482)
                     /locus_tag="ACHLCY_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0372187.1"
                     /translation="MRKLYAAILSAAICLTVSGAPAWASEQQATLSAGYLHVSTNAPG
                     SDNLNGINVKYRYEFTDTLGLVTSFSYAGDRNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     G"
     gene            complement(553..>2274)
                     /locus_tag="ACHLCY_005154"
     CDS             complement(553..>2274)
                     /locus_tag="ACHLCY_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0372188.1"
                     /translation="PEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQ
                     VLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQLAPGNYRLTVRA
                     VNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKR
                     IADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGQPSDD
                     ASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLTEIRTSITDVSNEITQTVNKKLED
                     QSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIENTPDGMQSQVLLA
                     ADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNPPAFSLTPDGRLT
                     AKNADISGSVNANSGALNNVTINQNCTIKGMLEATQVRGDFVKAVSKAFPKKVGTWGN
                     TETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGTRYTGYGFEVRKN
                     GVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGNITDCTVIVTKKA
                     ASGISIR"
BASE COUNT          485 a          666 c          543 g          580 t
ORIGIN      
        1 ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac ttccggtcag cacatcgtgc
       61 gttttcccct tgttgtcagt tacgcggaga taatccccgg agaaagtcga cacacggctg
      121 taagccactc ccgccatcgc atacgcgctg aaccattcat tcacgcgcac agacggcccc
      181 gccattacgc tgaaccagcg gttacgcacg gaatcttcat gccagcgggt atcgctgtaa
      241 cgggtaatct ggcgattcct gtctcctgca tagctgaatg acgtcaccag ccccagcgtg
      301 tccgtgaatt cataacggta tttcacgtta atcccgttaa gattatcgct accgggagcg
      361 ttcgtcgaga catgaagata ccccgcgctc agcgtggcct gctgctcaga cgcccatgca
      421 ggcgcaccgg atacggtcag acaaatggct gcggacaaaa tggcggcata aagtttacgc
      481 ataattacct ctcgcttttc tgcaataaaa aaggcgtcat tcctgacgcc ctttattggg
      541 gttataaata tttcaacgaa tactgatgcc ggaagcagct tttttggtca caatcaccgt
      601 acagtcggtg atattgcctg ccccctgatt gcctttctgg aaaatcttaa actccagagt
      661 gacgctacca ccaccactag gcatatcaat aactgcactg taactaccgg gaatggcccc
      721 tttagtttct ctggatgcga ttaatacgcc gtttttgcga acttcaaaac cataccccgt
      781 gtatcgcgtg cctcctgggt tatttccgct ccccggatcg tcatacgcta taccgttaaa
      841 aataatgggc ggaataataa tctggcggtc aaagttatga tcatcgctga tggtgactgt
      901 aaccgtaccg tttggtgttt ccgtgttacc ccacgtaccg acttttttcg ggaaggcttt
      961 tgatacagct ttaacgaaat cccctctgac ctgggtcgcc tccagcatgc ccttaatcgt
     1021 acagttctgg ttaatcgtga cattgttgag cgctcctgag ttcgcattca cactgccact
     1081 gatatccgca tttttcgccg tcagtcgccc gtctggtgtc agggaaaatg ccggaggatt
     1141 accgccgctg gtaatggtgg gagccgtcag atatttcagg aacacttcat tcatgaatat
     1201 ctgatcgccc tgaccaacaa acatcggctt tgtgttgcca ttcgcaggat taatcatcgc
     1261 aatcctgtct gctgccagca gcacctgact ctgcatgccg tcgggggtgt tctcaatacc
     1321 ggcaccgata cccgcaatat aaaggcgtcc gtcctgcatc tgctgcagct tcactgccca
     1381 catgctgttc aggttattat ttgtatcaac ctgaaccttc tgtatctgct ggatcgctgc
     1441 actctggtct tccagtttct tattgacggt ctgtgttatt tcattgctga catccgttat
     1501 ggacgtcctg atttcagtca ggtcaggcgc aagctgaccg ttatcaatct gcgtccacag
     1561 ctcctgagcc agatgggttt tccctatctc gcctttgaaa aaatccagat agcctgatgc
     1621 atcatcactc ggctgaccga cagcctctac aaatgccgat ttgccaacgg tgttcacact
     1681 gcggatataa aagtaataat catggcccgg tttgatattg atactggcag ctatccagta
     1741 cagcgccgtg ccaagatagc gggctgtggt ttcaacctgc ctgatatcag caatccgctt
     1801 ttccgagaac cagaactcaa actgtaccgt cggatcataa accgcaagat gcggcgtggc
     1861 ggttatctga aaatagcccg gtgtcagctc aatccgagac ggcgctgccg gtgcggcaat
     1921 ccggaacgat accgatgccg gatcgccctg ctgtccccac gcatttaccg cccggactgt
     1981 caacctgtag ttccccggcg ccagttgcgt gaagcggtat gtggtttctg tcgtcctggc
     2041 cgtgctgacc agccgctcac tgccgtcatc cgctgccacg gtcaggcgaa gcaggaagct
     2101 cacgcccttc accaccttcg gcgtgtccca gcgcgccagc acctgatatt ccccgctgtc
     2161 tgcggtgact tcggcggtca ggtgctgcac cgctggcggc gtgacaccat tcaccgtgcc
     2221 gctctggtcg ccgtcaaagt gcgccccgtt atccacgatg gcttcttttt ccgg
//
LOCUS       DAZGTR010000296         2227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGTR010000296 DAZGTR010000000
VERSION     DAZGTR010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.298"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..514)
                     /locus_tag="ACHLCY_005155"
                     /pseudo
     CDS             complement(1..514)
                     /locus_tag="ACHLCY_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
     gene            complement(474..593)
                     /locus_tag="ACHLCY_005156"
                     /pseudo
     CDS             complement(474..593)
                     /locus_tag="ACHLCY_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            complement(606..791)
                     /locus_tag="ACHLCY_005157"
     CDS             complement(606..791)
                     /locus_tag="ACHLCY_005157"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372189.1"
                     /translation="MIGANGRSVPEMALPESYNYIHKSGTLHEAPSPIIPLNWSKASM
                     TLMLKEMSNLINDEGIK"
     gene            complement(1076..2221)
                     /locus_tag="ACHLCY_005158"
     CDS             complement(1076..2221)
                     /locus_tag="ACHLCY_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFP0372190.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKELSRF"
BASE COUNT          579 a          492 c          404 g          752 t
ORIGIN      
        1 tccaattaca aaatgaatgg accaatataa tgattttttg tctaacactg gagcgtttcc
       61 gtattggcag actgtgactt tatctaacca aagattcaat tcccctgttc atattcttat
      121 tagtggttga gcagggctca atactccatc aaatacttta cctacgtgca aacgattata
      181 ttttagttca ggatgcttta gatttaacgg ggaactggat aaactgagcc attcccctat
      241 taacctttcc gtttctggca ttaaagccat cccagcgagc tcagatgaaa ttaagggaaa
      301 tatttctcca agcaataccc atccagacca gctgccactc tcttctgcta tcggtaacca
      361 tttagcacta aattcgggca ttaataatgc aacatcttcg ccattttctt tccaattttg
      421 aaaagtggtt tcaaacgtat gagtgtttcg atttactttc ctcagaccga acattaactt
      481 tcatccttct gatcgttttc gaatttgctt tcattttctt gctcctcata aggctgatgt
      541 cgttgtcctt gtcgttcatc ttgctcagtc aatacccaac gttgtgctgt aactcattat
      601 gaaaactatt taattccctc atcattgata aggttggaca tctctttcaa catgagagtc
      661 attgaagctt tcgaccaatt tagcggaatg atagggcttg gcgcttcatg caaagttccc
      721 gacttgtgga tatagttata gctttcggga agtgccattt caggtacact cctgccattt
      781 gcaccaatca tattctcgcc tgttatttgg gccaatgatc tattcataaa ttgcactgag
      841 tcattcaaat actcttcctt tcttgattct ttataaagaa ttgctgctga ttttgcatac
      901 catgagtcaa aaaaccattc tgcttctgtt gatggaatga agctcttttc tcttttcgca
      961 tgtgaatttt gatcggtatc ggttttaata tcattgaacc aaaaatttgc cgactgataa
     1021 ttatccttct cgtatctttt tataccatac ggacctgcta gttttttaac tatttttaga
     1081 acctgcttag ctcttttctt ctggtgttta tctttgcaag atttgcagga taaataacat
     1141 taagtaaagc cgcatcggct tctctgtaat gtttatcttt ttccagataa cctggggatt
     1201 ctccccctaa atcaagctgt aacgttattc tttcataacc tttatctatg aggtggttca
     1261 acctcgttgt tgaaagaggt tcatccagtt cattggcttt agcttcgcgt agtaaatcag
     1321 agacaaaaac agagtctttc ttcgagagta agttcgacaa acgttccagt ccagaggtca
     1381 ccagtgcaac ggaagatgtg tttaatcggg catcttcttc ccatgctccg gaatcttcca
     1441 taacatagaa attggcttta tccagataag caatcagcag cgcaaccgat tttaatcggt
     1501 cacctttttg ccaatcttca gcgttaattg tatcagtgtc gattgcttgt ataaggagat
     1561 caagataaag acctaacgca tcattttgtt tatggttcca cagctgcggc ttgccttctt
     1621 cctgcacatc tgccatcacg ggggagttgc tgtcaaatcg gatatgcaca acattcattt
     1681 gcccgggaac tccatccagt cgtttggggt tgctaatgac gtcctgcatc cgcttaatct
     1741 ggtcgggcgt agacatataa tcccacaggg tgagcagaac cttttttgcg gctacactat
     1801 ttccctgatc tgatactaat gccatatagc cccataaact atccctcagc cagacagcat
     1861 catagtgggt ttctgccgct gttttgtcat cttcgaaagt agaagcaaga ataaacgttc
     1921 cccagggttt ctccgtcaac gttaactttc gtgtgttttc tgttaatgtt agcaatcctt
     1981 gaatttcgtc tttggtatag tggctcttta tttcattttg taatttggct gagtgaatca
     2041 aaagacgata attaggatac ttatctggat ttaacgataa ataactttta tctgttgctg
     2101 cccccgtgga attaagaatg ttaaatacag attcactcgt cacttctgcc cctgtcgcct
     2161 ggcatgtgcc gcacgaaaat ataccagcga gcagaagagt taatgcagat ttccctttca
     2221 taaaata
//
LOCUS       DAZGTR010000297         2216 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGTR010000297 DAZGTR010000000
VERSION     DAZGTR010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.299"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            30..533
                     /locus_tag="ACHLCY_005159"
     CDS             30..533
                     /locus_tag="ACHLCY_005159"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0372191.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(579..995)
                     /gene="rraB"
                     /locus_tag="ACHLCY_005160"
     CDS             complement(579..995)
                     /gene="rraB"
                     /locus_tag="ACHLCY_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP0372192.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1157..2161
                     /gene="argF"
                     /locus_tag="ACHLCY_005161"
     CDS             1157..2161
                     /gene="argF"
                     /locus_tag="ACHLCY_005161"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP0372193.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYN
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          563 a          533 c          561 g          559 t
ORIGIN      
        1 taattacttt atttttattg ggttaaacta tgaataacat tgcgccgcaa tcacctgtaa
       61 tgcgtcgcct gacgcagcag gataatcccg ccatcgcccg cgtcattcgt caggtatccg
      121 ccgaatacgg tcttaccgct gataaaggct acaccgtcgc cgatccgaac ctggacgagt
      181 tgtatcaagt gtatagtcag ccgggtcatg catattgggt ggttgagtac gatggtgaag
      241 tggtcggcgg cggtgggatt gcgccattaa ccgggagtga gtcggacatt tgcgaactgc
      301 agaagatgta ttttcttccg gctattcgcg gtaaagggct ggcgaaaaaa ctggccttaa
      361 tggcgatgga gcaggcacga gagatgggct tcaaacgctg ctatctggaa acgaccgctt
      421 ttttgaaaga agcgattggt ttgtatgaac atttaggttt tcaacatatc gactatgcac
      481 ttggctgcac gggccatgta gattgtgaag tgcggatgct acgcgaactc taaaaaaatg
      541 cccgctagtg tgtgctatcg ggcattttaa tcaattaatt agtggcgaac cccgtcatcg
      601 tcttcatcga caaaatcttc atcgtcgccg tcttcgccat tgggatcttc aaagtaagtg
      661 ccccaaccgt cgtactcaac gtcaaatttc tccgccagcg tcatcagttg ttcaacctgg
      721 gcatcgatca gatcggcatt caacgcgcac tcgctgagga tgtcgcagca aatcacgata
      781 tcaccatctt caacttccag ctcttctgga tcggtcactt cgtaaccgag tttaaacgct
      841 tcaactgctg ctttttccag ggtttccaga tcgtctgcgg aaagatggtg ttcgatggtg
      901 tacagcgcgt ccggatcgct gccatcttcc agtaattctt caataatcaa acgtgtttct
      961 tcacgctgtt cttccagttg ttccgggttt gccatggctc attcctctta atgtcctgcc
     1021 gatactctta ttgtcacaca ccactgacat tgcctccacc tttgtgacaa agatttatgc
     1081 tttagacttg caaatgaata atcatccata taaattgaat tttaattcat taaggcgtta
     1141 gccacaggag ggatctatgt ccgggtttta tcataagcat ttcctgaaat tactcgattt
     1201 cacgccagct gaactcaaca gcctgctgca gttagccgcg aagctgaaag ccgataagaa
     1261 aagcggcaaa gaagaagcca aactcactgg taaaaacatc gcgctcatct tcgaaaaaga
     1321 ctcgacccgt acccgatgct ctttcgaagt tgccgcatat gaccagggtg ctcgcgttac
     1381 ttatctcggc ccaagcggca gtcagattgg tcataaagag tcgattaaag acactgcccg
     1441 cgtgctgggt cgcatgtata acggtattca gtatcgcggc tatggtcagg agattgtcga
     1501 aacactggcg gaatacgctg gcgtgccggt atggaacggc ctgaccaatg agtttcaccc
     1561 cacgcagttg ctggcggatc tcctcaccat gcaggagcat ttacccggta aagcattcaa
     1621 cgaaatgacg ctggtctatg caggtgacgc acgtaacaac atgggcaatt cgatgctcga
     1681 agctgcggcg cttaccggtc tggatttgcg tctggtcgcg ccacaggcat gctggccgga
     1741 agctgcgctg gttacggaat gccgcgccct ggcacagcaa aatggcggga atattacgct
     1801 gacggaagat gtagccaaag gagttgaagg tgctgacttt atctataccg atgtatgggt
     1861 gtcgatgggg gaagcaaaag agaaatgggc agaacggatt gcattactgc gtgattatca
     1921 ggtgaacagc aagatgatgc agttgaccgg taacccggag gtcaaattcc tccactgcct
     1981 gcccgcgttt catgacgacc agacgacgct tggtaagaaa atggcggaag agtttggcct
     2041 acatggcgga atggaagtga ctgatgaggt cttcgaatct gccgccagca ttgtttttga
     2101 tcaggcggaa aaccgcatgc ataccatcaa agcggtgatg gtcgcgacgc tcagtaaata
     2161 attgatatcg ctataaagat gccgggaaat acaaaaacat cccggcatca cattat
//
LOCUS       DAZGTR010000298         2212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGTR010000298 DAZGTR010000000
VERSION     DAZGTR010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.300"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1197
                     /gene="escC"
                     /locus_tag="ACHLCY_005162"
     CDS             <1..1197
                     /gene="escC"
                     /locus_tag="ACHLCY_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP0372194.1"
                     /translation="YLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKY
                     ITSLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMAS
                     NGSLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQR
                     QIEISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMI
                     RVNALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRV
                     TPRILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEAT
                     LSAGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPI
                     KSASSE"
     gene            1211..1666
                     /gene="cesD"
                     /locus_tag="ACHLCY_005163"
     CDS             1211..1666
                     /gene="cesD"
                     /locus_tag="ACHLCY_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFP0372195.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(2050..>2212)
                     /gene="grlA"
                     /locus_tag="ACHLCY_005164"
     CDS             complement(2050..>2212)
                     /gene="grlA"
                     /locus_tag="ACHLCY_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFP0372196.1"
                     /translation="RLEIFIYDVNLEAVPIESPGSTGPKRKTYRVGNGIVGQSNIWNE
                     MILRRKKES"
BASE COUNT          727 a          425 c          411 g          649 t
ORIGIN      
        1 tatctggata ttgactcact gctaaaatat ctgagtgata caatctcagt aaacaaaaac
       61 tcttgcaatg tgagaaaaat tacgacattt aactcgattg aagttcgggg tgttcctgaa
      121 tgtataaaat atattacatc tttatcagaa agtcttgata aagaggcaca gtctaaagcc
      181 aaaaataaag atgttgttaa ggtctttaaa ctgaactatg cgtcagcgac agatataaca
      241 tacaaatacc gtgaccaaaa tgttgtcgtc ccaggtgttg tctccattct gaagacaatg
      301 gcaagtaatg gttcgcttcc cagtaccggt aaaggtgccg tcgagcgaag cggtaacctg
      361 tttgataaca gtgtaacgat ttccgctgat ccacgtctta atgcagttgt tgtaaaagac
      421 cgcgaaataa cgatggatat ctatcagcaa ttaatttctg agcttgatat tgaacaaagg
      481 caaattgaaa tttctgtctc aattattgat gttgatgcaa acgatcttca gcagctcggc
      541 gtaaactggt ccggtacgtt aaatgcagga caaggtacga ttgcctttaa tagctctacc
      601 gcacaggcta atatcagctc ttctgtgata agtaatgcca gtaattttat gattagggtg
      661 aatgcgttac aacagaactc gaaagctaaa atactctctc agccgtcaat tataacctta
      721 aataatatgc aggcgattct tgataaaaac gtaacgtttt atacaaaagt ctccggagaa
      781 aaggtggcgt ctctggaaag tattacaagc ggaacattgc tgagagtgac accacgtata
      841 cttgatgaca gctcgaatag tcttaccgga aaacgtcggg aacgagttcg tttgttactt
      901 gatattcagg atggcaatca atcgactaac caaagcaatg ctcaggatgc cagcagcacc
      961 ttacctgaag tacaaaattc agaaatgaca acagaagcga cattaagtgc aggagaaagt
     1021 ttattacttg gcggatttat ccaagataaa gaaagttcaa gtaaagatgg tataccgctg
     1081 ttaagcgaca ttcctgttat tggtagttta ttctccagta cggtaaaaca aaaacatagt
     1141 gttgttcgac tctttttaat taaagcaacc ccgataaaat ctgcatctag cgaataataa
     1201 atgaggcttt atgagcagga aatttagctc tctagaggat atttatgatt tctaccagga
     1261 tggtggcaca ttagcgtcat taacaaatct gacacaacaa gatctcaatg accttcattc
     1321 ttatgcctat acagcatatc aatctggtga tgttataacc gcaagaaatc tattccattt
     1381 gctcacatat ctggaacact ggaattatga ctacacctta tctctgggct tatgccatca
     1441 gcgtttatca aatcatgaag atgcacaact gtgtttcgca cgctgtgcaa ctttagttat
     1501 gcaagatccc agggcatctt attattctgg aattagctac ttactcgtcg gaaataagaa
     1561 aatggccaag aaagccttta aggcttgttt aatgtggtgt aatgaaaaag aaaaatacac
     1621 tacatataaa gaaaatatta aaaaattgtt aggtaataca gagtaaaaac taacatcagc
     1681 tataccaatc cccaggctga ctggggattt tctatcggaa ataataaagc tgctatagtg
     1741 gagtgctccc aaagttgagc accgaccact ggtagattta tctacaggtg tggggttatt
     1801 ccattaacta ggattttatg ccctgaacta tcttctgctg tatttaatat catttccgtt
     1861 tatgaaacaa tcagtatatc ttaacttagc attagaaatc tgatctttcc aatcatctat
     1921 agctcagagt atatttgtca ggaaatgacc ttcaaacttt agccgagaaa aaggcttacc
     1981 ctggaaaaca aaacccttaa atatagcttt atttttattc ttctataaaa tatactcaaa
     2041 aaattacgtc taactctcct ttttcctcct caagatcatt tcgttccaga tattagactg
     2101 tcccacaata ccattaccaa ctcggtaggt ttttcttttt ggtccggttg atccaggact
     2161 ttctatcgga accgcctcaa ggttaacatc ataaatgaaa atctcaaggc gc
//
LOCUS       DAZGTR010000299         2172 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGTR010000299 DAZGTR010000000
VERSION     DAZGTR010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.301"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..283)
                     /locus_tag="ACHLCY_005165"
     CDS             complement(<1..283)
                     /locus_tag="ACHLCY_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscC/YscC/HrcC family type III secretion system
                     outer membrane ring protein"
                     /protein_id="HFP0372197.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRV
                     ILNDFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHLTWYYD"
     gene            complement(283..738)
                     /gene="sepD"
                     /locus_tag="ACHLCY_005166"
     CDS             complement(283..738)
                     /gene="sepD"
                     /locus_tag="ACHLCY_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFP0372198.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSKEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQSSSSDTMMPPTHLFSDIGRIV"
     gene            complement(744..1316)
                     /gene="escJ"
                     /locus_tag="ACHLCY_005167"
     CDS             complement(744..1316)
                     /gene="escJ"
                     /locus_tag="ACHLCY_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFP0372199.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(1319..1696)
                     /gene="sctI"
                     /locus_tag="ACHLCY_005168"
     CDS             complement(1319..1696)
                     /gene="sctI"
                     /locus_tag="ACHLCY_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP0372200.1"
                     /translation="MNNINQSKNINIQLNKAFQTNVVDEHIPLASTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(1779..2075)
                     /locus_tag="ACHLCY_005169"
     CDS             complement(1779..2075)
                     /locus_tag="ACHLCY_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFP0372201.1"
                     /translation="MEAANLSPSGTVLPLAATINGNNLVDEKTGVMQPEGETSRNVRI
                     LAGVLISVGTLAAIGTGIASLCVEDSSQRLGLGIAAGVLGGVTTVAGGLAMRYA"
BASE COUNT          612 a          412 c          408 g          740 t
ORIGIN      
        1 catcataata ccatgtgaga tgatagagtt tggacagttt ttccagtact tttacaggtt
       61 tttcattttt tatttcgcca gagaaatcat cattcacgga actactgatg aaaacaggaa
      121 tgctgtagtt agcagcaaag tcattaagga ttacacgtac tggagaactt ttagtaataa
      181 taaaatattc gttcttgcct aatctttttt ccagagagga cggggcagct tgtgcactgc
      241 agcaaaatag tgctgtaaaa ataaaaaaac ttattttttt cattacacaa ttcgtcctat
      301 atcagaaaac aagtgtgttg gcggcatcat tgtatctgat gaagatgatt gctctagttt
      361 atcaaattgc ttgtcgagca acgaggcgag agtatgttgc attattgctt ttgacatgtt
      421 tgcgcgatga ttattatctt tatgaaaaat ttggcaaaga taccacttct tattagacag
      481 gaaaataccc atttgtttat attctttact aacggccagg atattgcaga gtctgataaa
      541 tgtatctgtt ggaacctgac gaaaatcacc actaagctcg atatatagaa cccagtagtt
      601 tccaaaatcg tgcaagaaag catttttttg gtagatataa aaagtgagat gcgttggtga
      661 accgttgaca tcctttacaa aatcaagggc cgttagccag tcacaatcat tctttgctat
      721 gccattatta ttgttcatac atattacccg tcctgccctg aggatgactt gataacaaca
      781 ctaatatttt ctaatttgag atcgtcgaca ctattcttaa ccaggttttt tatctgaatg
      841 accgatggtg ctaagttaac ctctggcgag ctgatgacta aaacggctgc tgaggatggt
      901 tgactttcgt tattgttaac atttaatgaa acgctgcaat cgataacacc gggtatttta
      961 cttagcaacc tttcgatgtc ttgttctttg agatagttga tttttgcatt ttcttgtgat
     1021 ggcgatgcca ctaactggct gggtgggaaa ataacctcga tatctgcaaa tttctttttc
     1081 gggaagccgt tattatttaa aatcgttatt gcgcgaacaa aatcctcttt ttccacactg
     1141 agtgtcatat ttccactttt gtccatttct ttggaaacat tgacatcatt ggatagcaac
     1201 agtgcttgca tctgattagc ttctttttct gttaatcctg tatacaattg ctctttacat
     1261 gccactgtca aacaaatagc agccaataaa aaaaggtttt taatgtgttt tttcattttt
     1321 attgcatcga aactaatttt gtaaccgatg ttgaaaggag gcttgctaac ttagatacga
     1381 catcaacatt gacgtttgcg ttgagcacat tcttttgtaa agtgagtaca tcttcgggcg
     1441 agagcccacc tgcagatagt gcatcgaaac tgagatcggt gttcattgtt gcaagatgtc
     1501 gctttttaat ttcttcaatc aacacttgtt ctggggaaga cactcccgcc gttttaggga
     1561 gtaattgatc aagaaactga gccgctccgg ctgcagaggg ggttgatgct aacgggatat
     1621 gctcatcaac gacatttgtt tggaatgctt tatttaactg aatattaatg tttttcgatt
     1681 gattaatatt attcatattt actgttagtc tcttttcatg agagaggcaa tggcataatg
     1741 catccattac ctctctattt ttttatgcga tgcgatgatt aggcatatct catcgctaat
     1801 ccgccggcaa cagtagttac gccacctaat acaccggctg cgattcctaa gcctaatctt
     1861 tgtgaagagt cttcaacaca cagtgatgcg ataccagtac cgatagcggc tagagtacct
     1921 acacttatca aaactccagc taggattcta acgttacgac ttgtttcacc ttctggttgc
     1981 atcactcctg ttttctcatc cactagatta tttccattga ttgtggctgc cagtggtaat
     2041 actgtaccag aaggacttaa atttgctgct tccattgatc tttctccttt tgtctaattt
     2101 agacatttac ttggttataa ataaccaaat tatcccatag gcaactatta tattatttaa
     2161 atagttgcaa tg
//
LOCUS       DAZGTR010000300         2157 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGTR010000300 DAZGTR010000000
VERSION     DAZGTR010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.302"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..482)
                     /locus_tag="ACHLCY_005170"
     CDS             complement(<1..482)
                     /locus_tag="ACHLCY_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0372202.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDELNGINVKYRYEFTDTLGMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNELFSAYAMAGVSYSRVSTFSGDYLQVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     G"
     gene            complement(550..>2157)
                     /locus_tag="ACHLCY_005171"
     CDS             complement(550..>2157)
                     /locus_tag="ACHLCY_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0372203.1"
                     /translation="TVEVSAADGQYLAQAKWDTPKVVKGVSFMLRLTVVADDGSERLV
                     STARTTETTYRFTQLAPGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGY
                     FQITAVPRLAVYDPTVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHD
                     YYFYIRSVNTVGKSAFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAP
                     DLAEIRTSITDVSNEITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKD
                     GRLYIAGIGAGIENTPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFL
                     KRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKGKM
                     PANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGAKH
                     EREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAWLV
                     NNWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          478 a          645 c          507 g          527 t
ORIGIN      
        1 ccccacgcca gagacgtgtt gctgtggcga ccgtcatcac ttccggtcag cacatcatgc
       61 gttttcccct tgttgtcggt cacctgcaga taatctccgg agaaagtcga cacacggctg
      121 taagacacac ccgccatcgc atacgcgctg aacaattcat tcacgcgcac agacggcccc
      181 gccatcacgc tgaaccagcg gttacgcacg gaatcttcat gccagcgggt atcgctgtaa
      241 cgggtcagct ggcgattcct gtctcctgca tagctgaatg acgtcaccat tcccagagtg
      301 tccgtaaact cataacggta tttcacgtta atcccgttca gttcatcgct gccaggaacg
      361 ttcgtcgaga catgaagata ccccgcgctc agcgtggact gatgttcaga cgcccatgca
      421 ggcgcaccgg atacggccag acaaatggct gcggacaaaa tggcggcata aagtttacgc
      481 ataattacct ctcgcttttc tgcaataaaa aaggcgccat ttctggcgcc cgtatatggg
      541 ttataaaatt cagctgatac tgatgcctgc ggtggctttc ttcatcacca caaccagcaa
      601 atcgctgata cttgctgtgg gataccagtt attcaccagc catgctgata ccgaaaactc
      661 cagcgtcatg tgaccgtgac cggcaggcat atcaataacg ccactgtaaa tcagcgtatt
      721 atccagcgcg gtacggttat aaatttcagc accgtttttc cgcactatca gacggcatga
      781 ggagtaaata tcagtatgct ctctctcatg cttagcgcca ctgaatgcca ccgccggaat
      841 aacaatctgc cggtcaaacg gctgatcgtc ataaaccctg acggtaatgg tccctgatgg
      901 ccaccgctcc ggtgcccggg agtcccgcgg aaaagcctta cccactgttt tgactatatc
      961 gccttcaatc tggttggctg gcattttccc cttaatctga cagttctcat taattgtgac
     1021 attgttgagc gtcccggcgt tcgcattcac actgccactg atatctgcat ttttagcggt
     1081 cagctttccg tccggtgtca gggaaaatgc cggaggatta ccgccgctgg taatggtggg
     1141 agccgtcagg cgtttcagga acacgtcgtt catgaatatc tgatcgccct gaccaacaaa
     1201 catcggcttt gtgttgccat tcgcaggatt aatcatcgca atcctgtctg ccgccagcag
     1261 cacctgactc tgcattcctg ctggcgtatt ctcaataccg gcaccgatac ccgcaatata
     1321 aaggcgtccg tccttcatct gttgcagctt cacagcccac atgctgttca gattattatt
     1381 tgtatcaacc tgaaccttct gtatctgctg gatcgctgca ctctggtctt ccagtttctt
     1441 attgacggtc tgcgtgattt cattactgac atccgtgatg gacgttctga tttccgccag
     1501 gtcaggcgca agctgaccgt tatcaatctg agtccacaac tcctgagcca gatgggtttt
     1561 ccctatctct cctttgaaaa aatccagata gccggatgca tcatcactcg gctggccaac
     1621 agcctccaca aatgccgatt tgccaacggt gttcacactg cggatataaa agtaataatc
     1681 atggcccggt ttgatattga tactggcggc tatccagtac agccccgtgc caaggtagcg
     1741 ggctgtggtt tcaacctgcc tgatatcggt aatccgcgtt tccgaaaacc agaactcaaa
     1801 ctgtaccgtc gggtcataca ccgcaagacg cgggaccgct gttatctgaa aatagcccgg
     1861 tgtcagctca atctgtgacg gcgctgccgg tgcggcaatc cggaacgata ccgacgccgg
     1921 atcgccctgc tgcccccacg catttaccgc ccggactgtc agcctgtagt tccccggcgc
     1981 cagttgcgtg aagcggtatg tggtttccgt cgtccgggcc gtgctgacca gccgctcact
     2041 gccgtcatcc gcgaccacgg tcaggcgaag cataaagctc acgcccttca ccaccttcgg
     2101 cgtgtcccat ttcgcctgcg ccagatactg accgtcagct gcgctcacct ccaccgt
//
LOCUS       DAZGTR010000301         2139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGTR010000301 DAZGTR010000000
VERSION     DAZGTR010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.303"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            217..1206
                     /locus_tag="ACHLCY_005172"
                     /pseudo
     CDS             217..1206
                     /locus_tag="ACHLCY_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1289..1615
                     /locus_tag="ACHLCY_005173"
                     /pseudo
     CDS             1289..1615
                     /locus_tag="ACHLCY_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1756..>2139)
                     /locus_tag="ACHLCY_005174"
     CDS             complement(1756..>2139)
                     /locus_tag="ACHLCY_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0372204.1"
                     /translation="LAWKADDRMLFVAKERRPSKIFIYQLSPDLLSVKQATIPEELND
                     IRINDISGLAFNNESLMILSDESRNLLKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEG
                     IVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          612 a          472 c          526 g          529 t
ORIGIN      
        1 ttgcaggatc acatcacgta ttttcctgaa aacgcaggct gcttagtagc aaagcagaag
       61 ttcaaaatca ccaactaaat agctgcgccg aatagtagat caatgaaagg gaactcagcc
      121 cggattgtgc gatctgatca atcgccaaaa atccccccct aaccggactg agcgatgccg
      181 atcatagcac caattccccg cgacgaatga cgcctgatgc aaaaagcaat ctataaaact
      241 cacgataaaa attatgcccg caggctgacc gccatgctga tgctgcaccg gagcgctcgc
      301 gtcagtgacg ttgccagaac gctctgtttc gcccgttcat ctgtcgggcg ctggattaac
      361 tggttcacac agtcgggtgt tgatggactg aaatcattac ccgccgggcg tgcccgccgc
      421 tggtcatttg aacatatctg tacactgtta cgtgagctgg taaaacattc tcctggcgac
      481 tttggctacc agcgttcacg ctgaagcaca gaactgttgg caataaaaat caatgagata
      541 accggatgtc agttacatgc cggaaccgtg cgccgctggt tgtcgtctgc ggggctggtg
      601 tggcgcagag cagcgccaac tctgcgtatc cgtgacccgc ataaagatga aaagatggtg
      661 gcaattcata aagcactgga cgaatgcagc ccagagcatc cggtatttta tgaagatgaa
      721 gtggatatcc accctaatcc caaaatcggt gcgggctggc agcttcgcgg acagcaaaaa
      781 cacgtggtga cgccgggtca gaatgaaaaa tattatctgg ctggcgcgtt acacagcgga
      841 acgggaaaag tgagttatgt gggtggcaac agcaagagct ccactttatt tatcagtctg
      901 ttgaaacacc tgaaatcgac ataccgacgg tctaaaacta tcatgctcat cgtggacaac
      961 tacatcatcc acaaaagccg ggaaacacag cgctggctga agcagaatcc gaagttcagg
     1021 gtgatttatc agctggttta ctctccatgg gtgaatcacg ttgaacggct atggcaggcg
     1081 cttcacgaca caataacgca caaccatcag tgccgttcaa tgtggtagtt gctgaaaaag
     1141 attcaccatt ttatgggaac cgtcagccca ttccccggag gtaattatgg acaggcaaaa
     1201 gtgtagcggt attaggcaca gctatttagt attaagacta tcacttattt aagtgatatt
     1261 ggttgtctgg aaataagggg gcaagaacaa gtggctgacg gataatggtt catgctaccg
     1321 ggcgaatgaa acacgtcagt tcgccaggat gttgggactt gaaccgaaga acacggcagt
     1381 gcggagtccg gagagtagcg gaatagcaga gagcttcgtg aaaacgataa agcgtgatta
     1441 cataagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg cagaggcgtt
     1501 cgagcattat aacgaatggc atccgcatag tgcactgggt tatcgctcgc cacgggaata
     1561 tctgcggcag cgggcttgta atgggttaag tgataacaga tgtctggaaa tataggggca
     1621 aatccactgg ttgataatag ctgatgtcct tttcagctat taatgtgttt attttagcgt
     1681 aaaattatac tcgtattttt ataactttat gagattgcca gatttggtcg ataaataata
     1741 atgccagtga cagacttact tattagggac aaatttcgcg aggatatcag gttcactggc
     1801 aacataaatg ctttcatcag acagagttac aattccttca ggttggggca aatcactggt
     1861 cagagaatga tttccttttg tcaggtcaag catctcaatg aaagacatct ctgtcaggtt
     1921 aaacttgagt aagttacgcg attcatcgga cagaatcatc agactttcat tattaaaggc
     1981 caggccggat atatcgttaa tacgtatatc atttagttcc tctggaatag ttgcttgctt
     2041 tacactcagc aggtctggag aaagttgata tataaatatt ttacttgggc gtctttcttt
     2101 tgcgacaaac agcatccgat catctgcttt ccatgccag
//
LOCUS       DAZGTR010000302         2114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGTR010000302 DAZGTR010000000
VERSION     DAZGTR010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.304"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..191
                     /locus_tag="ACHLCY_005175"
     CDS             <1..191
                     /locus_tag="ACHLCY_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0372205.1"
                     /translation="GVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYL
                     TNLYFKIREDRRKVARGE"
     gene            complement(447..719)
                     /locus_tag="ACHLCY_005176"
     CDS             complement(447..719)
                     /locus_tag="ACHLCY_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372206.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            879..1412
                     /locus_tag="ACHLCY_005177"
     CDS             879..1412
                     /locus_tag="ACHLCY_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0372207.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1633..1746
                     /locus_tag="ACHLCY_005178"
     CDS             1633..1746
                     /locus_tag="ACHLCY_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372208.1"
                     /translation="MKGIEVETPASLDLIRAAAFAIRIVAIAVLVWAIRWW"
     gene            1748..2053
                     /locus_tag="ACHLCY_005179"
                     /pseudo
     CDS             1748..2053
                     /locus_tag="ACHLCY_005179"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2001..>2114
                     /locus_tag="ACHLCY_005180"
     CDS             2001..>2114
                     /locus_tag="ACHLCY_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFP0372209.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDWQT"
BASE COUNT          542 a          433 c          586 g          553 t
ORIGIN      
        1 cgggtgtgtc atacaccacg tcagcggtgg ggacgggata ctggttactg cagctgctgg
       61 acaaagtctc tccgtcccag tgggtggcga taggtgtgct ggggagtctg ctgtttggcc
      121 tgctgacgta tctgactaac ctgtatttca aaatcagaga ggaccgtcgt aaggtggcgc
      181 ggggagagta gtcgatgaat aaacaatacg aactggttgt aaaatgaata tttctaactg
      241 aaaaaacgtt ccatgaggta agaaaaggtc acaggcaatc aataacagga cgtgatgaaa
      301 gacccttgca tttgtgcgct ttctctttag atagcagcag atactgaaaa tctgagttgt
      361 cggggagtca gggatacagc tgtgcaagag ttggtcattg tgattccatt gaaatcctgt
      421 atgccatgaa gggcaggatt ttatggctac ctgagctttg gtgatagtaa gttgaaaatt
      481 cgcatttttt gctgacatgc gtaacgagaa tcccataaac agggaggact taattcttca
      541 ttaacccatg cgttgatatt atgtttcagc cgttgaagca tcagcggtgt taatgttgtg
      601 gtaataatat ccagcgtttt atgtgagatc ttaccgtaag ggtctgcaag aatgctgctt
      661 gttgcttcgt tattatctgc catcagaaga agtaactctg atttaacgtt ttctgtcatt
      721 agttgtaaaa atcttctgcg caaactttct ttactgttca tttatatggc ttcatttgtt
      781 gtaatctgct gcgtctcaag ggatatgttt atgagagcga ccatgagtgt ttgattatat
      841 acctaacata tcaagggatt agaaatcgat aaatccccat gaacgaaaaa ataaaatacg
      901 gcctgtcggc tgccgttctg gcgctgattg gtgcaggtgc ttctgcgcct gaaatcctcg
      961 accagtttct ggatgaaaag gaaggtaacc acaccacggc ataccgtgat ggtgcgggga
     1021 tctggaccat ctgccgtggt gccattctgg tggatggtaa gcctgttatt cctggcatga
     1081 agctgtcaaa ggaaaaatgc gaccgggtta atgccatcga acgtgacaag gcgctggcat
     1141 gggtggagaa aaacatccgg gtgccgctga ccgaacccca gaaagcgggg atcgcgtcat
     1201 tctgtccgta caacattggc cccggtaagt gcttcccgtc gacgttttat aaacgaatta
     1261 atgcaggcga tcgaaaaggt gcctgtgagg cgattcgctg gtggattaag gacggtggca
     1321 gagactgccg tatccgttca aataattgct acggtcaggt ctcacggcgt gaccaggaga
     1381 gcgcgctggc gtgctgggac atcgacagat agcagaatat tttcctgaaa aatgacgttg
     1441 gccaacgcgg gtggataaca cgaaatcctg aaaactggta aaacctaagt gaataaaagt
     1501 aaaaaacccg tttgttggca gcaagcgggg ttttgtgttt tctgaccttg agtaaggcaa
     1561 gggagaaatt atgggtaggg aggtactttc cctgtgagga agtataaaag attctttctg
     1621 aggttgtcca ttatgaaagg cattgaagtg gagacgccag ccagtctgga tttaataaga
     1681 gcggcagctt ttgccattcg tattgtggcc attgctgttc tggtctgggc aatccgttgg
     1741 tggtgatatg aaccgtgttc tgtgcgtggt tatcattgtc ctgctggtgg cctgtggtgc
     1801 gcttagtctg gggctgaatc attaccgtga tcacgccatc atctacaaag agcagcgcga
     1861 taaaaaagcc agtgagctgg agctggcgaa cgcgacaatt actgatatgc agatacgcca
     1921 gcgtgatgtc gctgcacttg atgccagata ctcgagggaa ttagccgatg cgagagctga
     1981 aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag cgcctgcgga tcaacgccac
     2041 ctgctccggt accgtgcgtg aagccaccgg cacctccggc gtggataatg caaccggccc
     2101 ccgactggca gaca
//
LOCUS       DAZGTR010000303         2090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGTR010000303 DAZGTR010000000
VERSION     DAZGTR010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.305"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..867
                     /locus_tag="ACHLCY_005181"
                     /pseudo
     CDS             1..867
                     /locus_tag="ACHLCY_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1386..>2090
                     /locus_tag="ACHLCY_005182"
     CDS             1386..>2090
                     /locus_tag="ACHLCY_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytotoxin"
                     /protein_id="HFP0372210.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHEQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNLLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSD"
BASE COUNT          681 a          422 c          449 g          538 t
ORIGIN      
        1 tgcgcccgtt cctccgtcag gcgctggatt aactggttca cgctgtcgag tgttgaaggt
       61 ctgaaatcat tacccgcagg acgttcccgc cgctggccgt ttgagcatat ctgcacattg
      121 tttcgtgaac tgataaaaca ctctcccgga tattttggct atcagcgttc acgctggagt
      181 acagaactgc tggcaataaa aatcaacgag ataaccgagt gccagttaca tgccggaacc
      241 gttcgccgct ggttgccatt agtcgggctt gtgtggcgca gggctgcgcc aaccctgcgt
      301 acccgtaacc tgcataaagc agcaattcac aaagcactgg acgaatgtag tgcggagcat
      361 ccggtctttt atgaagatga agtgtatatc tatcttaatc ccaaaaccgg tgcggactgg
      421 caacttcgca gacagcaaaa acatgtggta acaccggggc agaatgaaaa atactatctg
      481 gctggtgctt tacatagtgg aacgggcaaa gtcagctatg tgggtggcaa cagcaaaagc
      541 tcagccttat ttatcagtct gttgaaacat ctgaaattga cataccgacg ggataaacct
      601 atcacgctca tagtggacaa ctacatcatc cataaaagtc gggaaacaca gcgctggctg
      661 aaagaaaatc cgaagttcag agtgatttat cagccagttt actcgccgtg ggtgaatcat
      721 gttgagcggt tatagcaggc gcttcacgat acgataacgc gcaatcatca gtgccgttca
      781 atgtggtaac tgcttaaaaa tgttcattat tttatggaaa ccgtgagccc attccccggt
      841 ggtaaatatg ggctattaaa agtgtagcgg tattcggcgc agctatttag gatggtcagg
      901 tcgcagaagt tctgtgccac tacagtgcgt gtactaaaca aaatgacgag ggcacgaatg
      961 tcataatacg tgcgtattac ctgacagtag ctaccaactg agggggctta ttccaaacct
     1021 gatttattca acaaagctgg acttatcctt actctgaatt attttccaga catatgcatc
     1081 atgtactggc tcgcttggtt caggaaatac aggagcaaat ctacagctat tagatttatt
     1141 cctatttgta tgcaaagtcg cattgcataa tgcggagcta tgttacagat acgcctgacg
     1201 tgcgtagtaa aattataaag caacatatat atacaaggag aaaccgagaa aaataaatga
     1261 gtcgtatgtt gggtagcatc gagataaata tttttttatc acattagcac cttggttaag
     1321 ggtgttaata ataatattct ctcacttatt aaaacataaa taatttttta agcggaaatg
     1381 taattatgag actgccagag aaagttcttt ttcctcctgt cactagtggc ctgtcagggc
     1441 aggaaaaaca aaaaaaaccg aagagcatta ccggatttca ggaaaattat caacgcaata
     1501 tcaggccaat caaaacagca tcagaagccc gactacgctt ctttgataaa atggtttcga
     1561 aagaaaactc tctggaagat gttgtttctt taggtgaaat gattcagaag gaaatttatg
     1621 ggcatgaaca aagaacattt tcaccagttc atcatacagg taactggaaa tcatcattgt
     1681 tacacaacgc gctccttggt ctggcaaatg tttataatgg cttacgggaa acagaatacc
     1741 ctaacacttt caacagagat ggtataaaaa gtactaactc ttttagagat aacttattga
     1801 caaaaacaag aactcccaga gataattttg aggaaggaat aaaacatcct gaacatgcaa
     1861 caataccata tgacaacgac aatgaaagta ataaattgct aaaagcagga aagatagctg
     1921 gtaacaataa cgagctgttg atggaaataa aaaaggaatc ccaaagcgac catcaaatcc
     1981 ccctgtcaga taagttcctg aaaaggaaaa aacgatctcc tgtagctgaa gataaagttc
     2041 aaaactcgtt aacaccagaa aattttgttc agaaaatttc acttagtgat
//
LOCUS       DAZGTR010000304         2089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGTR010000304 DAZGTR010000000
VERSION     DAZGTR010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.306"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            62..442
                     /locus_tag="ACHLCY_005183"
     CDS             62..442
                     /locus_tag="ACHLCY_005183"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0372211.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            439..787
                     /gene="tnpB"
                     /locus_tag="ACHLCY_005184"
                     /pseudo
     CDS             439..787
                     /gene="tnpB"
                     /locus_tag="ACHLCY_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060295.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            837..>2089
                     /locus_tag="ACHLCY_005185"
     CDS             837..>2089
                     /locus_tag="ACHLCY_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP0372212.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGRITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQ"
BASE COUNT          483 a          582 c          609 g          415 t
ORIGIN      
        1 atctgacgaa ttagacaaag tggtgtccac caaataagta gtgggaacca aagtgtcaga
       61 tatgcagaaa aatgtgaccc ccggcaggcg aaaaggctgc cctaattatc ctcccgaatt
      121 taaacagcag ctcgttgctg cctcctgtga acccgggata tccatctcaa aacttgctct
      181 tgaaaatggc attaacgcca atctgttgtt caaatggcga caacaatggc gcgagggaaa
      241 gctgctatta ccttcttcag agagccccca gctacttcct gtgactctcg atgcagctgc
      301 cgaacagcca gaatcgctcg cagaggaccc ggaaaccctc agtatcagct gtgaggtaac
      361 gttccggcac gggacgctcc gcttcaatgg caatgtcagc gaaaagctcc tgactctgct
      421 gatacaggaa ctgaagcgat gatcccgtta ccttccggga ccaaaatttg gctggttgcc
      481 ggtatcaccg atatgagaaa atggcttcaa cggcctggct gcgaaagtac agacggcgct
      541 gaaagacgat cccatgtccg gccatgtttt cattttccgg ggccgcagcg gcagtcaggt
      601 taaactgctg tggtccaccg gtgacgggct gtgcctcctg accaaacggc tggagcgtgg
      661 gcgcttcgcc tggccgtcag cccgtgatgg caaagtgttc cttacgcagg cgcagctggc
      721 gatgctgctg gaaggtatcg actggcgaca gcctaagcgg ctgctgacct ccctgaccat
      781 gctgtaaatc tctttatcct ggttgtcaca gaataagccc ggtaaaatac gggcttatga
      841 acgacatctc ttctgacgac atcttcctgc tgaaacagcg cctggccgaa caggaagcgc
      901 tgatccacgc cctgcaggaa aagctgagca accgggagcg cgaaatagac catctgcagg
      961 cgcagctgga taaactccgc cggatgaact tcggcagtcg ttccgaaaaa gtctcccgcc
     1021 gtatcgcaca aatggaagcc gatctgaacc ggcttcagaa agagagcgat acgctgactg
     1081 gtagggtgta tgacccggca gtacagcgtc cgttgcgtca gacccgcacc cgtaagccgt
     1141 tccctgaatc actaccccgt gacgaaaagc gactgttgcc tgcggcgccg tgctgcccga
     1201 actgcggcgg ttcactgagc tatctgggcg aggataccgc cgaacagctg gagttgatgc
     1261 gtagcgcctt ccgggttatc cggacggtac gggaaaaaca tgcctgtact cagtgcgata
     1321 ccatcgtgca ggcacctgca ccttcgcggc ccatcgagcg gggtatcgcc ggaccggggc
     1381 tgctggcccg cgtgctgacc tcgaagtatg cagagcacac cccgctgtat cgccagtcag
     1441 aaatatacgg ccggcaaggt gtggagctga ggcgttcact gctgtcgggc tgggtggatg
     1501 catgctgccg gctgctgtct ccgctggaag aggcgcttca tggctatgtc atgactgacg
     1561 gcaaactcca tgccgatgat accccggtcc aggtactgct gccgggtaat aagaagacga
     1621 agaccgggcg gttgtgggcg tatgttcgtg atgaccgcaa tgccgggtca gcgttggcac
     1681 ctgcagtgtg gttcgcttac agcccggaca ggaaaggcat ccatccgcag actcatcttg
     1741 cctgcttcag cggtgtgctg caagcggatg cgtacgccgg gttcaacgag ctgtatcgca
     1801 atggtcggat aacggaagct gcctgctggg ctcatgcccg ccgaaagatc cacgatgtgc
     1861 acgtccgcat cccgtcagca ctgacggaag aagccctgga gcagatcggt cagttgtacg
     1921 ccatagaggc ggatataagg ggaatgccgg cagagcagcg gcttgctgaa cgtcagcgaa
     1981 aaacgaaacc gctgttgaaa tccctggaaa gctggttgcg tgaaaagatg aagaccctgt
     2041 cgcgacactc agagttggcg aaggcgttcg cgtacgcact taaccagtg
//
LOCUS       DAZGTR010000305         2080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGTR010000305 DAZGTR010000000
VERSION     DAZGTR010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.307"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..425)
                     /locus_tag="ACHLCY_005186"
     CDS             complement(<1..425)
                     /locus_tag="ACHLCY_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0372213.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAII"
     gene            complement(560..757)
                     /locus_tag="ACHLCY_005187"
     CDS             complement(560..757)
                     /locus_tag="ACHLCY_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0372214.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(981..1535)
                     /locus_tag="ACHLCY_005188"
     CDS             complement(981..1535)
                     /locus_tag="ACHLCY_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP0372215.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIDKKVIAF"
     gene            complement(1544..1903)
                     /locus_tag="ACHLCY_005189"
     CDS             complement(1544..1903)
                     /locus_tag="ACHLCY_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0372216.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVSGGRLGVKIYKIESE"
     gene            complement(1916..>2080)
                     /locus_tag="ACHLCY_005190"
     CDS             complement(1916..>2080)
                     /locus_tag="ACHLCY_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0372217.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT          559 a          557 c          414 g          550 t
ORIGIN      
        1 tgaattatcg ccctgacttt ggcacttatg ctggataaac atccacttaa aaacaatgac
       61 atccgttcat ctgtttcaaa cagagacagg caggccatca taagaaacat atccgtgacc
      121 tctccggaaa atccctggct ggtaattaaa gccgcagcca acgcaggatt gttgggttcc
      181 agcaacaggt aaagcggaat gtcagtcaga cgagtcctcg tcagcttatg ctgactttcc
      241 agatattgac ttacgacgga ttcgcttata tcgacaattt taactttgcc ataatgcata
      301 aggaaaagct ccctgatgca tttggtggcc agaacgactg agcctggctt aagtgacaac
      361 gtatcctttt caagaaaaat attaattggg gagcaaacca tgataactga acagacaaca
      421 gccattataa ttttactttc attagcaatt ggttagctca attatagccc caaaaggtaa
      481 attatcatca acacataagc aaaggactga caggtgtcgc ccccccacca gccgcccatt
      541 caccacaaat aaaaagcctt caggactgaa ggcgtctgta acaaccgcac tgatagtctg
      601 ccagacccgc cataacaagc tgggtcagta ttaactggca gcgttcgcgt gaaaggtaag
      661 tattctgcgc aatctccccg actgtcgccg gttcggtaac gcttaattca ttaaacacca
      721 ctctggcggt ttctgtcata tcctgctgtt ttagcatgtc ttttttccct tttggttaac
      781 gtgacatacc aataactctt gtccaaaaag ccagcaagca gaaagaccgg tattcgcaac
      841 caccagcgca tttaacgccc cgtgccgttt ttcagtcata aaaaaacccg caaaaagcgg
      901 gctctttcaa atgtccatgt ctgctattcg cctcgcggta cagctttgcg aagcgtaccg
      961 gaattgaagc agtttttacg tcaaaaagca ataacttttt tatctatacc aaaagccata
     1021 accattggtt tgtacaaaat aaattctgcc acctttagcc aatgctcaat gcgtctttca
     1081 caggttctta aactccattc cggatgtgca tcattcagca gttcagccat tttgcgctta
     1141 gtcatccccc gccccacata acgctgactc agaacattga gcagcccggg ataacctgcc
     1201 agtacttcac caataaccct gtcgattatt aacgcctctg aatcggtaca atgtgccagc
     1261 cagctttttt gattgccgtt gatcatatcc cgcaaaaaag cctcaagttc aggtttgtcc
     1321 agacccgctt ttttcatcct ccggagcgcc tcgttaattg ccgtttttgt cagcttttta
     1381 gaggtcagta attggttgaa catatttccc gtcttaccgt cgccaatata cgaccaacgc
     1441 ccccacatac gcagtttccc ctggatccag acactttcca gcgttttcag gcgtaaatgc
     1501 tcaccgcttt tgcctgtaat ttccgggtat atcatattta tgctcactca ctttcaattt
     1561 tgtaaatctt cacgcccagc cgcccaccag aaacgagctg gccgcgcaca atattgattt
     1621 catcaaactg ctcgtcgtct atgagaagtc cggcatgcgt cagcgcatcc agtggtgctt
     1681 tcaggatatt gtccaggtca cgacggcgct tatccggtgg ctctgcaata atctttatcg
     1741 ccagccttcc ggacaggttt aatttcagcc gctgctggcg aacaataagt gccacatccc
     1801 ggcgataacg ctcaccggct tttgatacaa aatatgtgct gccacgacgt cgccagtagg
     1861 tgttcaccgt cggcgggtaa ggcaaaacaa actctatacg catcagtaac ctcttttacc
     1921 cgagcacgcc ggttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgggaac
     1981 catgcttttc ttcgaacgcc agcggatccg catgaagctc gttgtgatgc tcccgacaca
     2041 gcggtagcgt gaaaatatcg tgggattttg ttcccattcc
//
LOCUS       DAZGTR010000306         2073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGTR010000306 DAZGTR010000000
VERSION     DAZGTR010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.308"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            158..373
                     /locus_tag="ACHLCY_005191"
     CDS             158..373
                     /locus_tag="ACHLCY_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0372218.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKTARGD"
     gene            378..722
                     /locus_tag="ACHLCY_005192"
     CDS             378..722
                     /locus_tag="ACHLCY_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP0372219.1"
                     /translation="MKKKYELVVKEINNYPDKIAVTVALEIGGHPSLLLPHVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            773..1306
                     /locus_tag="ACHLCY_005193"
     CDS             773..1306
                     /locus_tag="ACHLCY_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0372220.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1580..>2073
                     /locus_tag="ACHLCY_005194"
     CDS             1580..>2073
                     /locus_tag="ACHLCY_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP0372221.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFR"
BASE COUNT          568 a          432 c          580 g          493 t
ORIGIN      
        1 catcataaaa agtgagccag tcaaatggaa ggtatcgtta aactcaccgg tagtgtcagt
       61 gggtcgtctg agacgcctgc atgagttatc agagccatca gtagttaact ggtggctttt
      121 ttattgttgt cagcttccgg ataacgggag acggggtatg taccagatgg aaaaaatcac
      181 aacaggtgtg tcatacacca cgtcagcggt agggacggga tactggttac tgcaactgct
      241 ggacaaagtc tctccgtccc agtgggtggc aataggtgta ctggggagtc tgctgtttgg
      301 cctgctgacg tatctgacta acctgtattt caaaatcagg gaggaccgcc gtaagacggc
      361 gcggggagac taaagcgatg aagaaaaaat acgaactggt tgttaaagag ataaataatt
      421 acccggataa gattgctgtt actgtggcac ttgaaattgg cgggcatccg tcgttgttgt
      481 tgccacatgt ggcgattagt cttgaccgta ctgaaggtgc cacgctggag ttttacgaag
      541 ctgaggcgaa aaagcaggcg aagcagtttt tcatggatgt tgctgccggg ttatgtgaag
      601 gggatggtcc gttaccggaa aagcgtcccg taattttaga ggcgcaggat gtgttgataa
      661 cctacagagg aaaactaccg ggaataatta cgggttctct gaagactcca ccgctggcct
      721 gaagacttaa catatccagg gatttgaaat cgataaaccc tgataaatat ccatgaacgc
      781 aaaaatcaga tacggcctgt cggctgccgt tctggcgctg attgccgcag gtgcgcctgc
      841 gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt aaccacacca cagcataccg
      901 tgatggtgcg ggtatctgga ccatctgccg tggagccacc cgggtggatg gtaagcctgt
      961 tattcctggc atgaagctgt cgaaggaaaa atgcgaccgg gttaacgcca ttgagcgtga
     1021 taaggcgctg gcatgggtgg agaaaaacat cagagtgcca ctgaccgaac cccagaaagc
     1081 ggggattgcg tcattctgtc cgtacaacat tggccccggt aagtgtttcc cgtcgacgtt
     1141 ttataaacga attaatgcag gagatcgaaa aggtgcctgc gaagcgattc gctggtggat
     1201 taaggacggt ggcagagact gccgtattcg ctcaaataac tgttatggtc aggtatcccg
     1261 tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac agataagcag aatattttgc
     1321 tgaaaaatga cgttggccag cgcgggcgga taacacgaaa tcctgcgaac tggcaaaatg
     1381 taagtgaata aagttaggca gattatttca cgcagaggca ccgtaatggt gcctttgtca
     1441 tttctgcgct tcgcacaagc gtaaataaac caaagaacct ttcaggatga gccctggtgg
     1501 ataaccggca gtggtctggt taaccctctt tgggctggtt attcctgtgc gcagggttca
     1561 tcactaaaag gaatcaacca tgaaagagat gatttctgtc gatcatgaaa tatccatgag
     1621 tagtctggat tttctgaata acattattaa tccagcccgg gcagaagccg gagaagtccc
     1681 tcatgaaccg cgtaagtttc ttgcaaaaat tgaggatgag ctagagcttg atggaaccgg
     1741 aaaaaaattc cggttaaaca ataaccagac aagaacggca tactatgatc tggattttga
     1801 ccagatgatg ctcgttggca tgagggagtc aaaggccgtt cgtcgttctg tgctggcaag
     1861 actcaaagcg atgcatggta ttcagatccc ccggacttta cctgaggcgt tgcgatttgc
     1921 ggcaaaactg gctgaacaga aagcagtgct ggaaaatcaa ctggcaatag cagcgccgaa
     1981 ggctgaattt gttgataact atgttgaagc atctggtctg atgggattcc gggaagttgc
     2041 taagttactc ggtatcaaag aaaccgattt ccg
//
LOCUS       DAZGTR010000307         2063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGTR010000307 DAZGTR010000000
VERSION     DAZGTR010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.309"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..406)
                     /locus_tag="ACHLCY_005195"
     CDS             complement(<1..406)
                     /locus_tag="ACHLCY_005195"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP0372222.1"
                     /translation="MNVIDLFSGVGGLSLGAARAGFDVKMAVEIDQHAINTHAINFPR
                     SLHVQEDVSLLNAEIIKGFFKNDMPIDGIIGGPPCQGFSSIGKGNPDDSRNQLYMHFY
                     RLVSELQPLFFLAENVPGIMQEKYSGIRNKAFN"
     gene            complement(1000..1104)
                     /locus_tag="ACHLCY_005196"
                     /pseudo
     CDS             complement(1000..1104)
                     /locus_tag="ACHLCY_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1162..1323)
                     /locus_tag="ACHLCY_005197"
     CDS             complement(1162..1323)
                     /locus_tag="ACHLCY_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372223.1"
                     /translation="MTTITKERIELFIKNPVENGLTRGEQMELARIALASLEAEPVGD
                     FYEYKPDDW"
     gene            complement(1334..1597)
                     /locus_tag="ACHLCY_005198"
     CDS             complement(1334..1597)
                     /locus_tag="ACHLCY_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372224.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            complement(1599..1816)
                     /locus_tag="ACHLCY_005199"
                     /pseudo
     CDS             complement(1599..1816)
                     /locus_tag="ACHLCY_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            complement(1849..2061)
                     /locus_tag="ACHLCY_005200"
     CDS             complement(1849..2061)
                     /locus_tag="ACHLCY_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372225.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
BASE COUNT          562 a          500 c          357 g          644 t
ORIGIN      
        1 aattaaatgc tttatttcta atgccagaat atttctcttg cataatacct ggaacatttt
       61 ctgccaaaaa gaataatggc tgtaattctg atactaaacg gtagaaatgc atgtaaagct
      121 gattcctgct atcatcagga ttccccttgc ctattgaact aaatccttgg cacggaggac
      181 cgccaataat accatctatg ggcatatcgt ttttaaaaaa acccttaatt atttctgcat
      241 ttagtaaaga aacatcttct tggacgtgca aacttcttgg aaaattaatt gcgtgagtat
      301 taatagcatg ttgatcaatt tcaactgcca ttttaacatc aaatccagca cgtgcagcac
      361 caagacttag acctccaacg ccagaaaaca aatctattac attcatatag ttgcaacgtc
      421 tcttattagt ttctatgtat tataacacaa ctgttcttga tgttttaacc ccataagata
      481 acctcataaa ggctatccct tgaaaatacc ggatcgcata aatttaatct ttcaaaacta
      541 cttgtctatt aatcgagcaa tttaaggttg ttttagtcat gtcgataccg acatagttaa
      601 ctaaacccat ttcatcgtcc aacaaatggg ggtaatacac tttgatttat agtcgggtgg
      661 gtttttctct atctgccttt ggtgttcatg cccaaggcag atagcctcaa gcacccgcag
      721 caattctact taactatcct tttcccgcaa atcgttttta tccccagcgg caaatcgaat
      781 acacaaccag cgccaccgcc atcgcaattc ctaccgttgt gaatgcttca ggccaggtca
      841 tcgtgaaaca tcctccacgc ttataagtcc gcttcgctcc aggtagtcca tcactttatc
      901 cggcaatttg cagcccggtt tcgctttctt cagttgacta accaattgtt taaccagcat
      961 tgtcaattcg cgaacttgtt tcccgggctc ccctttgccc tgaagcaggg cagcgcggca
     1021 agcgttccat ccctcagcat atgtttcagt tacaccatcg agatggcatg taagcaaatc
     1081 catttcttcc ggcactacgg gcgctggaaa aacggcatag ggtggcgtcc attttggcgc
     1141 tttatctcca gccgaacgct gctaccagtc atccggtttg tattcataaa aatcaccaac
     1201 tggctctgct tccagcgatg ccagcgcaat ccgtgccagt tccatttgtt caccacgggt
     1261 aagcccgttt tcaaccggat ttttaatgaa caattcaata cgttctttgg taatagtggt
     1321 catgtgttac tccttaaccc gcagtgcttt caactgatga ggggaacaaa atcttttcat
     1381 caaaccctgc attcatatca tgaacagcaa cacaccaatc catcgacgaa cgattatcaa
     1441 gagcctccat gatttcatcc atgcggcgta ggtcatacag gtaaatgctt ttatcgccaa
     1501 tggtgtaaaa gccaattttt ttcggtgatg gacagcgatc aagaacttcc tgtaattcgt
     1561 tcaaccatgc ccgttctttt tttgtcaaag ttgccatatc agttttcctt atacggatta
     1621 attttattgt gcagtgtgtt gaatgacgcc cataccacgt cgttatacaa ttcaataact
     1681 ggctcaatta tttttccgat tatccagact aggattaacg gggatatcgg tatcatcaac
     1741 acgataaaca gaatgagaaa cagaaattct gttgttctac tctttcgcgg atattttttc
     1801 taaataatgt gaccattcat taccgccctt tcgggcggcc tcctgacatt aatcgttgtg
     1861 ataactcata gcttcatttg cagcatcaac tggatcaacc tccctccagc aataatttgg
     1921 gtcggctcct tcaggcgtcc acggttctaa ttcatttttt gccgcattct catcgccagt
     1981 aattttaaaa atctgctcag agaattttct tgcccactcg ttatattttt ccgcattaat
     2041 ggctttctgt gtatttaaca taa
//
LOCUS       DAZGTR010000308         2058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGTR010000308 DAZGTR010000000
VERSION     DAZGTR010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.310"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1719
                     /locus_tag="ACHLCY_005201"
     CDS             <1..1719
                     /locus_tag="ACHLCY_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFP0372226.1"
                     /translation="SLILSGCGPESENKESQQQQPSTPTDQQVLAAQQAAIKEAEQRS
                     VADKATADAKAKALAQQEAQQYSDKQALQGRLQAAPKYQHAAREKAASQIANPGTARY
                     KQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNHGLLPPPDAVRVEEIVNYFPYD
                     WDIKDKQSIPATKPIPFAMRYELAPAPWNEQLTLLKIDILAKDHKSEELPASNLVFLI
                     DTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISGSHKAEINA
                     AIDSLDAEGSTNGGAGLELAYQQAAKGFIKGGINRILLATDGDFNVGIDDPKSIESMV
                     KKQRESGVTLSTFGVGNSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLNSEMRQTLI
                     TVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKHITLLFELT
                     LNGQKASIDKLRYAPDNKSVKSDKTKELAWLKIRWKYPQGKESQLVEFPLGPTINAPS
                     EDMRFCAAVAAYGQKLRGSEYLNNTSWQQIKQWAQQAKGEDPQGYRAEFIRLIELADG
                     VTDISQ"
     gene            complement(1790..>2058)
                     /locus_tag="ACHLCY_005202"
     CDS             complement(1790..>2058)
                     /locus_tag="ACHLCY_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFP0372227.1"
                     /translation="FVCMAAQEVIEQRESNSDSAPYTLLKNYADRFKKYSAEEQYEID
                     FQHRLVNRNCYLDKYGDARINASYTQLEIKHSQPKNRASGKRVS"
BASE COUNT          591 a          450 c          503 g          514 t
ORIGIN      
        1 agtttgattt tgtcaggatg cgggccggaa tctgagaata aggaaagtca gcaacaacag
       61 cccagtaccc ccacagacca gcaagtgctt gccgcgcaac aagctgcaat aaaagaggcc
      121 gagcaaagat ccgtcgccga taaagccact gcagacgcga aagcaaaagc cttagcccag
      181 caagaagcgc aacaatattc agacaaacag gctttgcagg ggcgcttaca ggcggctcca
      241 aaatatcaac atgcagccag agaaaaagca gcgtcccaaa tcgcaaatcc aggaaccgct
      301 cgctacaagc agttcgatga taatccggtt aagcaggtag cgcaaaaccc gttggcgacg
      361 tttagccttg acgtcgacac cggcagttat gcgaatgtac gccgttttct caatcatggg
      421 ctgttacctc cgccagacgc tgtgcgggtg gaggagatag tcaattattt cccgtatgac
      481 tgggatatca aagacaaaca atctattccg gccactaagc caataccttt cgctatgcgc
      541 tacgaactgg cacctgcgcc gtggaatgaa cagctaacat tgctgaaaat tgatatcctg
      601 gcgaaagatc ataaaagtga agagttacca gcttctaatc tggtctttct tatcgatact
      661 tctggttcaa tgatttctga tgaacgtttg ccacttatcc agtcttcgtt gaaattattg
      721 gtcaaagaac ttcgtgagca ggataacatt gccatcgtga cctacgctgg cgactcccgt
      781 attgcgttgc cttctatctc cggtagtcac aaggcggaaa ttaatgccgc aattgattcg
      841 ctggatgccg aaggcagtac caatggcggt gccgggctgg aactggctta tcagcaggcg
      901 gcgaaggggt ttattaaggg cggcatcaat cgcattttat tagccactga cggtgacttt
      961 aacgttggca ttgacgatcc aaaatcgatt gaatcaatgg tcaaaaaaca gcgggagtct
     1021 ggtgttactc tgtcgacgtt tggcgtgggg aatagcaatt acaacgaggc aatgatggtg
     1081 cgaattgccg atgttggtaa cggcaactac agctacattg ataccctctc tgaagcgcag
     1141 aaagtattga atagtgaaat gcggcagacg ttgattaccg tagcaaaaga tgtcaaagcg
     1201 caaattgagt ttaaccccgc gtgggtaacg gaataccgtc agattggtta tgaaaagcgc
     1261 caacttcggg cggaagattt taataacgac aacgttgatg cgggggatat tggcgcaggc
     1321 aaacatataa cgttgttatt cgaattaacg ctgaacgggc aaaaagcatc aattgataag
     1381 ttacgctatg ccccggataa caaatcagtg aaatcggaca aaacgaaaga actggcctgg
     1441 ttaaagattc gctggaaata cccgcaggga aaagaaagtc agttagttga attcccgctg
     1501 gggccaacaa taaacgcccc ctctgaagat atgcgttttt gcgcagcagt agctgcatat
     1561 gggcaaaagt tacgcggttc tgaatacctg aacaatacct cctggcagca gatcaaacag
     1621 tgggctcagc aggcaaaagg ggaagatcca cagggttaca gggcggaatt tattcgcctg
     1681 attgaactgg cggatggtgt gactgacatc agtcagtgat gactgtttag caaactatgt
     1741 tcgaccagtc agcatatttg ctgactggtc gaattgatta acaatgatgt taactcactc
     1801 ttttgccgga tgctctattt ttgggttgtg agtgttttat ttctaattgt gtataagaag
     1861 cattgattcg tgcatcgcca tatttatcta aataacaatt tctgtttacc agtcgatgtt
     1921 gaaaatcaat ttcgtactgc tcttctgctg aatatttttt aaatctgtca gcatagtttt
     1981 ttaataacgt atagggggcg ctgtcagaat tgctttccct ttgttcaatc acttcctggg
     2041 ctgccatgca cacaaacg
//
LOCUS       DAZGTR010000309         2054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGTR010000309 DAZGTR010000000
VERSION     DAZGTR010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.311"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            326..1558
                     /locus_tag="ACHLCY_005203"
     CDS             326..1558
                     /locus_tag="ACHLCY_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0372228.1"
                     /translation="MRLSNIVTVIILAISSTLTPQAMAEKLIPETDAELLIWSDATSA
                     EYMKYAATEFNKDFGYKVKFTFRNIAPMDAASRIMQDGGTTRVADVAEIEHDTLGRLV
                     VAGGVMENMVSAERIKKTFIPGAVSAATYNNISYGFPVSFATLALFYNKDLLNTAPKT
                     FEEINTFSEKFNNSSEHKYALLWDVQNYYVSRMFITLYGANEFGKTGNDPKALGIASS
                     EAKKGLETMKRLKKANPSNPLDMGNPQVLRGLFNEGKVAAVIDGPWSIQGYIDSGINF
                     GVTRIPTLDGHQPRTFSTVRLAVVSSFTEYPHAAELFADYLTTDKMLMKRYEMTNLIP
                     PIDSLMNKISQTGSEAIKAIIAQANYSDAMPSIPEMSYLWSPMTNAILATWVENKTPD
                     EVLNHAQTIIEEQLSLQE"
     gene            1561..>2054
                     /locus_tag="ACHLCY_005204"
     CDS             1561..>2054
                     /locus_tag="ACHLCY_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP0372229.1"
                     /translation="MNTQQLKMKSAPVLPISCLIMGGTQLSRHYYVKGGIFFAIQVCF
                     LLYLSDIVHTLIGLFTLGDVAQIRKGLTVIQGDNSIFMLVEGVIATIIVGLFATIYIL
                     NIKDARNSSYCHLTFKQQLYKLYEDKFAFIVLTPAFLASIAFIVLPIVITVLVSFTNY
                     AAPN"
BASE COUNT          636 a          384 c          399 g          635 t
ORIGIN      
        1 aaaaggactg ttgcaattga agccgataag acattctttt ttattaaaac cataaatgag
       61 ttccttccaa aatatcaatt taagatatga tttctgttat tccatatagt cgtacctcat
      121 gtactcctaa acactctgcg gcaataaaat atttttttcc tcaacaaatt actgcaaatg
      181 agatatgcat cactatttgc agttattcat cattaaaatc ttatttgaca gttgattggc
      241 cttgatttgt gttgataatg gcgtgaatgc ttatgtaggc gtacagcatg tactacttaa
      301 agatattatt cacgaggtaa atataatgag actttcaaat atcgtcacag ttataattct
      361 tgcaatttcc agtacgctaa caccccaggc gatggcagaa aaattgattc ctgaaactga
      421 tgctgagtta ttaatctggt ctgatgccac aagcgcggaa tatatgaaat atgcagcaac
      481 ggaattcaat aaagattttg gctacaaggt caagtttaca tttcgcaata tagcgccaat
      541 ggatgcagca tcaagaatta tgcaggatgg gggtacgact cgtgtagctg atgtagctga
      601 aattgaacat gataccctgg ggcggttagt cgttgctggc ggggttatgg aaaacatggt
      661 ctcagctgag cggattaaaa aaacatttat tccaggcgca gtatcggcag ctacatataa
      721 taacatcagc tatggttttc ctgtaagttt cgcaacgctg gcgcttttct ataataagga
      781 tttgttaaac accgcaccaa aaacattcga agaaatcaat actttcagtg aaaagtttaa
      841 taattcatcc gagcataaat atgctctgct atgggatgta caaaattatt atgtttcacg
      901 tatgtttatt accttgtatg gtgccaacga attcggtaaa accggtaacg atcctaaagc
      961 tctaggcatc gcttcatctg aagcgaagaa agggctagag acaatgaaac gcttaaagaa
     1021 agcgaatcct tctaatcctc ttgatatggg taatccacaa gttctaagag gtctgtttaa
     1081 tgaaggaaaa gttgctgctg taatcgacgg accttggtcc atacaaggtt acattgacag
     1141 cggaatcaat tttggcgtga cacgcatccc aacattagat ggtcatcagc ctcgcacatt
     1201 ttcaacagta cgactggccg ttgtaagctc atttaccgaa tatcctcatg ccgctgaatt
     1261 attcgctgac tacctgacaa cggataagat gttgatgaag cgttatgaaa tgacgaatct
     1321 catcccgcca atcgattcac ttatgaacaa aattagccag acaggtagtg aagctataaa
     1381 agcaattatt gctcaagcta attattctga cgcaatgcca tcaataccgg aaatgtctta
     1441 tctatggtct ccaatgacta atgctatttt ggctacctgg gttgagaata aaacaccgga
     1501 tgaagtttta aatcatgcac aaacaattat tgaagaacaa ctttcgcttc aggagtaatt
     1561 gtgaatacac aacaactgaa aatgaagagt gcacctgtgc tacctatttc ctgtttgatt
     1621 atgggcggga ctcagttatc acgtcattat tatgtgaaag gcgggatatt ctttgcaata
     1681 caagtatgct ttttgcttta tctttccgat attgtgcata cattaatagg tttgttcaca
     1741 ttaggtgatg tcgcgcaaat tcgaaaaggg ttgactgtta ttcagggtga caattctata
     1801 tttatgcttg tagagggcgt gatagcgacc attattgttg gcttattcgc cactatttac
     1861 atccttaata ttaaagatgc gcggaactca tcttattgcc atctgacttt taaacaacag
     1921 ttatataagc tctatgagga taaatttgca tttatagtcc taacgcccgc atttttagcg
     1981 agtatagcat tcattgtttt acctattgta attacagttc ttgtatcgtt tacaaactat
     2041 gctgcgccaa atca
//
LOCUS       DAZGTR010000310         2034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGTR010000310 DAZGTR010000000
VERSION     DAZGTR010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.312"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(422..>2034)
                     /gene="nleL"
                     /locus_tag="ACHLCY_005205"
     CDS             complement(422..>2034)
                     /gene="nleL"
                     /locus_tag="ACHLCY_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector E3 ubiquitin
                     ligase NleL"
                     /protein_id="HFP0372230.1"
                     /translation="SFKNSILNECNFCYANLSNCIIRALFENSNFSNSNLKNASFKGS
                     SYIQYPPILNEADLTGAIIIPGMVLSGAILGDVKELFSEKSNTINLGGCYIDLSDIQE
                     NILSVLDNYTKSNKSILLTMNTSDDKYNHDKVRAAEELIKKISLDELAAFRPYVKMSL
                     ADSFSIHPYLNNANIQQWLEPICDDFFDTIMSWFNNSIMMYMENGSLLQAGMYFERHP
                     GAMVSYNSSFIQIVMNGSRRDGMQERFRELYEVYLKNEKVYPVTQQSDFGLCDGSGKP
                     DWDDDSDLAYNWVLLSSQDDGMAMMCSLSHMVDMLSPNTSTNWMSFFLYKDGEVQNTF
                     GYSLSNLFSESFPIFSIPYHKAFSQNFVSGILDILISDNELKERFIEALNSNKSDYKM
                     IADDQQRKLACVWNPFLDGWELNAQHVDMIMGSHVLKDMPLRKQAEILFCLGGVFCKY
                     SSSDMFGTEYDSPEILRRYANGLIEQAYKTDPQVFGSVYYYNDILDRLQGRNNVFTCT
                     AVLTDMLTEHAKESFPEIFSLYYPVAWR"
BASE COUNT          735 a          414 c          269 g          616 t
ORIGIN      
        1 ataattttcg ttgtaattaa aatcatattt catctatagt gaccattcac agcaaaacaa
       61 aatcaattat cacattcaaa aaaatactga caacaaagat actcttcaga caaagttgtc
      121 atatgtaaat tttcccttaa aacgttgctc aagaaaaata taaaaatcat atgccctaat
      181 caacatcctc aagcactacg atatatgcag tatcctattc atatattacc accatatcag
      241 acaaatcgaa tcacaacgag ttctatcgta ctctttcatc ttcgacactg taaattttaa
      301 gaacattcgg ctcatgccac taaaccaaga cataataaca acggagcaat aaatgttccc
      361 actgaacatg tgcaaaccta aaaagacagt gttacaataa tattcatcca tggtctctaa
      421 atcaacgcca cgcaacagga taatacaatg aaaatatttc aggaaaagat tcttttgcat
      481 gctccgttag catatcagtc agcacagcgg tacaagtaaa aacattattt cttccttgta
      541 gcctgtctaa aatatcattg taataataaa ctgagccaaa tacctgagga tctgttttat
      601 aagcttgttc aatcaatcca tttgcatatc tccgtagaat ctcaggagaa tcatactctg
      661 taccaaacat atcactcgat gagtatttac agaaaacccc ccctaaacaa aataatattt
      721 cagcctgttt tcttagtggc atatctttca atacatggct ccccataatc atatctacat
      781 gctgagcgtt cagttcccaa ccatcaagaa agggattcca gacacaggca agtttccttt
      841 gctgatcatc agcaatcatt ttataatctg atttattgga attaagtgcc tcaataaatc
      901 tctctttgag ttcattatca gaaatgagta tatccagaat accagaaacg aaattctggg
      961 aaaaagcttt atgataagga atactgaaaa ttggaaatga ttcagaaaaa agattgctca
     1021 atgaataccc aaatgtattt tgaacttctc catccttata taaaaaaaag gacatccaat
     1081 tagttgatgt attaggagat aacatatcaa ccatatgact caaagaacac atcattgcca
     1141 taccatcatc ctgtgatgat aacaaaaccc agttataagc caaatcggaa tcatcatccc
     1201 agtcaggctt cccagagcca tcgcacaatc caaaatcact ctgctgtgtg acaggataaa
     1261 ctttttcatt ttttaaatat acttcataga gttccctaaa tcgttcctgc attccatcac
     1321 gccgtgaacc attcattaca atttgtataa aggaactatt ataagatacc atcgcacctg
     1381 gatgtcgctc aaaatacatc cctgcctgca ataaactacc attctccata tacatcatta
     1441 ttgaattatt aaaccaagac attatagtat caaaaaagtc atcacatata ggctcgagcc
     1501 attgctgtat atttgcgttg ttcaaataag gatgaatact aaatgaatca gccaaagaca
     1561 tcttaacata gggccggaac gccgctaatt cgtcaagaga tatttttttg ataagttctt
     1621 cagcggccct tactttatca tggttatact tatcatcaga tgtattcata gtcaataaaa
     1681 ttgatttatt tgattttgta tagttatcca acacagataa tatattttcc tgtatgtcag
     1741 atagatctat gtaacaccct cctagattaa tggtattact tttttcacta aagagctcct
     1801 ttacatcacc taagatagca ccacttaaaa ccattccagg aattataata gctcctgtta
     1861 aatcagcctc gttcaaaatt ggaggatatt gtatatatga agatccttta aatgatgcat
     1921 ttttaagatt ggaattgctg aaattagagt tttcaaacaa agccctaata atacaattag
     1981 ataaattagc ataacaaaaa ttacattcat tcaaaatcga gtttttaaaa gaac
//
LOCUS       DAZGTR010000311         2008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGTR010000311 DAZGTR010000000
VERSION     DAZGTR010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.313"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            136..1368
                     /locus_tag="ACHLCY_005206"
                     /pseudo
     CDS             136..1368
                     /locus_tag="ACHLCY_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1353..1991
                     /locus_tag="ACHLCY_005207"
     CDS             1353..1991
                     /locus_tag="ACHLCY_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFP0372231.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
BASE COUNT          499 a          441 c          568 g          500 t
ORIGIN      
        1 ggatgtgata acaaatccaa caaccaggtt aatatctgaa gatttttgtt gttattattt
       61 atccccaaag cggtgagtta ttaatgaagt ttgcctgaat gtataaacag aattattcga
      121 cataaagcca ggaaaatgag tgatacgctt ttaaccgaaa aaatccttac cggagaaaat
      181 gttttgcgtg ctgcaattgc ccgcattgag tggatttttg aaacatttcc gtcggtatgt
      241 ctgtcttttt caggtggaaa ggattccacc gtgcttttcc atcttgtggc agaggtggcc
      301 cgcaggagaa aacgtcattt ctctgttctg ttcattgact gggaggccta gtatcggtgc
      361 accattgaac atattcagaa gatgcgggaa atgtaccatg atgtgacgga aaccttttac
      421 tgggtggcac ttcccctgac cacggtaaac ggcgtctcgc agtttcagcc ggagtggata
      481 tgctgggagc caggagtgac ctgggttcgc cagccaccgg aagaggccat tacggatatg
      541 acatattttc cctttttccg gtatgccatg acgtttgaag aatttgttcc ggcattttct
      601 tcctggtttg ccggtaaccg gtgtggagtg gcagtgctga ccggtgttcg tgcggatgaa
      661 tccctcaatc gctttatggg gctggtgtct cagcgcaaac tgagatatgc cgatgataag
      721 ccctggacca cggcatcgcc tgaagggttt tattacacca tgtatccgtt gtatgactgg
      781 aaagcccgcg atatctggat atataacgcc agagcttgtg ccatttacaa tcctctgtat
      841 gacctgatgt accgtgccga cgtgccgtta cgcaacatgc gtgtctgtga accttttggt
      901 cctgaacaac gtaaggggct gtggctttac catgttctgg agccggaaac ctgggccagg
      961 atgtgtgagc gggtgtcagg cgctgccagc ggggcgcttt atgccaatga aagcggtgcc
     1021 tattttgccc tgcgtaaacg tatatcaaaa ccagcccatc atacctggcg cagctatgcg
     1081 atgttcctgc tggatgtgat gccggaaaga acggcagaac attaccgtaa taaaattgct
     1141 gtctacctgc gctggtatca gacgcggggc ttcccggatg acatcccgga tgaacaggag
     1201 aatgacctgg ggagccggga tattccgtcc tggcggcgta tctgtaagac acttataaag
     1261 aatgatttct ggtgccggac cctctccttc agtccgaaca aaccccggca ctatgaacgt
     1321 tatctgcagc gtatgaaaga aaggaggaag gaatggggga ttctgtgaca ccggagataa
     1381 ttggcaacat gattcgtcag tacttcagcc aggaacgacc agaagaagag accatacagg
     1441 cccttaatca cctgcgcaga gtgcttcatg aagtcagtcc ttttgcacat gagccagtgg
     1501 actgtgtgct gtgggtaaaa gcagatgagg tggtggccaa tgattacaat cctaatgtca
     1561 tggcgccggg tgagaagaaa ctgctgaagc agtcactgga gaaagatggt ttcacacaac
     1621 cggtggtggt gtctgaggaa aagaaccatt acctggtggt ggatggtttt catcgccagg
     1681 tgctgggcag ggaagcaggt acgggaaaac gtctgaaggg ctggctgccg gtggtctgta
     1741 ttaatccgga gagaaaggga caggcctcac gtattgctgc gaccatccga cataaccggg
     1801 ccaggggcaa acatcagata acctcaatgt ctgatattgt cagggacctg tcgcgcctgg
     1861 gctggacgaa tgaacgcatt ggtacggagc tgggtatgga ccaggatgaa gttctcagat
     1921 tgaaacagat tagcgggctg acggagctgt ttcaggaaga ggatttcagc ccggcatgga
     1981 cggttcgctg agaccgtcag atatgtat
//
LOCUS       DAZGTR010000312         1970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGTR010000312 DAZGTR010000000
VERSION     DAZGTR010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.314"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(23..754)
                     /locus_tag="ACHLCY_005208"
     CDS             complement(23..754)
                     /locus_tag="ACHLCY_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0372232.1"
                     /translation="MKLDTFSQRLTYAMDQAGFTQASLGNAVGMSQPSVWKLTSGKTR
                     NTRKLFEISKVLGVRTEWLSDGTGPMRDEGVEPYNPKSSIPHESTWGYLDPWDGGTPL
                     RGDEVEIPYLKDIEFACGDGRVIDEDHNGFMLRFSKSTLRRVGANSDGSGVVCFPARG
                     NSMEPNIPDGTTVAVNTNDKKIVDGKIYAINENGWKRIKILFRSGPDKVSIRSFNSLE
                     YPQEEKNLSDIEIIGRIFWWSVVDY"
     gene            863..1093
                     /locus_tag="ACHLCY_005209"
     CDS             863..1093
                     /locus_tag="ACHLCY_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0372233.1"
                     /translation="MVNKAIKAAIDSVGSQQKLADACGVKQPSVWAWLHGKKRVSAEN
                     AKRIEMATNGSVPAYLIRPDLSALFPNPNKAA"
     gene            1213..1509
                     /locus_tag="ACHLCY_005210"
     CDS             1213..1509
                     /locus_tag="ACHLCY_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP0372234.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGLTNKKRPAATERSDQIQMEF"
     gene            1672..>1970
                     /locus_tag="ACHLCY_005211"
     CDS             1672..>1970
                     /locus_tag="ACHLCY_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0372235.1"
                     /translation="MSMNLMAKAMNIKVGNPLRKLVLIKLADNANDNGECWPSYQHVA
                     DQCEVSRSTVKSHIRALEDMGLLKREFRRKGELNQSNVFYLTLDNAQQIQPELGGA"
BASE COUNT          584 a          427 c          436 g          523 t
ORIGIN      
        1 ggtttgttgt ggtgtgatga ggttagtagt caactacaga ccaccagaag attcttccga
       61 tgatctcgat atcgcttaga ttcttttctt cttgtgggta ttccagtgag ttaaagcttc
      121 taatgcttac cttgtcaggc cctgaccgaa agagaatctt aatgcgtttc cagccgttct
      181 cgttaatggc gtaaatcttt ccgtcaacta ttttcttatc gttggtgtta acagcaactg
      241 ttgttccatc aggaatgttt ggctccatgc tgttgccacg agccggaaaa caaacaaccc
      301 cgcttccatc actgttcgcg ccaactctgc gaagggttga tttggagaag cgcaacataa
      361 agccgttgtg atcttcatca atcacccgac catccccgca tgcaaactca atatctttaa
      421 ggtaaggaat ttcaacttca tcacctctta aaggcgttcc tccatcccat gggtccaaat
      481 atccccacgt gctttcatgg ggaatagaag attttggatt ataaggttca actccctcat
      541 cacgcattgg cccagttcca tcggaaagcc attccgtacg aactccaagc acttttgata
      601 tttcaaaaag tttgcgcgta ttgcgtgttt ttccagacgt aagtttccag acgcttggct
      661 gagacatgcc aacagcattg ccaagagaag cctgagtaaa cccagcctga tccatcgcgt
      721 atgtaagcct ttgagaaaaa gtatctagtt tcattcgtac aacctatagc tacagctatt
      781 attagtcaaa tacctaaagc tatttacttt ctgaatagct ttggctatta tcctaattgt
      841 gaattcagca ggagttattt ttatggtcaa caaggctatt aaagcggcta ttgactcagt
      901 aggaagtcag caaaagttag ctgatgcctg cggtgttaag cagccgtctg tatgggcttg
      961 gttgcatggg aagaaaaggg tatccgctga aaatgccaag cgcattgaaa tggctaccaa
     1021 tggaagcgtc cctgcatacc tgattcgccc tgatttatcc gctttgttcc ccaatccgaa
     1081 caaagcagct taataaagca aatttttata ccgaacggcc cggtatacgg tcgggtgccc
     1141 ggcgtggtca tggatgactg tcaatggtgc acaataaaaa accaaattat ttacctatgg
     1201 aaatagtaag aaatggaaca aacaagttac agcaaactat cacagcgcga cgttgatcgc
     1261 gcagaaacag atttacttat caacctgtca acgcttaccc agcgcggtct ggcaaagatg
     1321 attggctgtc atgaatcgaa gataagcaga acggactgga gatttattgc ttcggtcttg
     1381 tgtgctttcg gaatggcatc agacatcagt ccgattagca gggcttttaa gtatgcgctt
     1441 gatggactca ccaataaaaa acgcccggcg gcaaccgagc gttctgatca aatacaaatg
     1501 gaattttaac aacatccaac gaggtaatta tatgcgaaaa acgcaggaaa ataaacgcgt
     1561 taatcaccga aaagatgtgc tacgtgacca gttttatcag ggggttaatc cagcaatagc
     1621 tgtgccactg agagaaatac ttaacaggta caaaacttcg gagaagtcaa aatgagcatg
     1681 aatcttatgg ctaaggccat gaatataaag gttggcaacc cactgagaaa actggttctg
     1741 attaaacttg ccgataacgc caatgataat ggcgaatgct ggccttcata tcaacatgtc
     1801 gctgaccagt gtgaggtgag cagatcgaca gtaaaaagtc acattagggc actggaagat
     1861 atggggcttt tgaaaaggga attcagaaga aaaggagagc ttaaccagtc aaacgttttt
     1921 tatctgacgt tggataatgc acaacaaatc caaccagaat taggtggggc
//
LOCUS       DAZGTR010000313         1951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGTR010000313 DAZGTR010000000
VERSION     DAZGTR010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.315"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..141
                     /locus_tag="ACHLCY_005212"
     CDS             <1..141
                     /locus_tag="ACHLCY_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372236.1"
                     /translation="VMCEGLFHGKKFCEACGTGIYCRATCTKYVYRYQPDLYQDCGGA
                     LT"
     gene            329..762
                     /locus_tag="ACHLCY_005213"
                     /pseudo
     CDS             329..762
                     /locus_tag="ACHLCY_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            844..1044
                     /locus_tag="ACHLCY_005214"
                     /pseudo
     CDS             844..1044
                     /locus_tag="ACHLCY_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1305..1781
                     /locus_tag="ACHLCY_005215"
     CDS             1305..1781
                     /locus_tag="ACHLCY_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372237.1"
                     /translation="MRRFVQQAVDHWPNLLAFHFTFHSTEGNIYGQQIHAFCISFYQQ
                     IHKRITARTHTTSPSSPLVLHWLLEQHGGAVIRCVLLLSQTSICHPRASVTVDEECSK
                     LVDLLQQIWPVISTGGQCQVERCFQVARGDTSEQYVALKTDALSFAVSDHKSSGAE"
BASE COUNT          473 a          462 c          502 g          514 t
ORIGIN      
        1 gtgatgtgcg aggggctttt tcatgggaag aagttctgtg aggcctgtgg tacggggatt
       61 tattgcagag caacatgtac gaagtatgtt tacaggtacc agccagattt gtaccaggac
      121 tgcggaggtg cactcactta atccgaaatg cccgctctgc tcagggcgtt caggctgact
      181 atcaatggca tacattttat ccggtgtttc gcacccacac cgtgcaacga ctcgacaccg
      241 gatatccgca gtcccgctta cctgaataaa acccggggga ttgattgtgt actgtggtgt
      301 gaaatcgcac cgtgatcgct gattcgccga atccgcactg tggtgtcaat caaccacacg
      361 cgagagtcgt ctcaggcttc gcctgcctca ggacgatttc tgcatagcta attcccgggg
      421 caacatttat agaggcgctg tgcgctctga tggtcgccac ttctgacccg gaccatgctc
      481 ctgatatgtc ggaatataat gtgatgttag gcatcagaga ggaatatttc gatgccccta
      541 atctggggcg tttgattctg gatgtctgga gcaatccggg ctttgtgtca ccggacgaat
      601 caaagcagta ccacccggga tgtggcgctg gatcgtcatt caccggactg tgaagacata
      661 cttaaagagc tgatatgggc gttcagtgat ttcgcccgcg atcatcgtgg tgtgtgtgat
      721 ccggaagccc gttacctcgc cggtaatcct tggtatccgt agcaggccag ctctgaaagc
      781 acagctcagc agattccccg cattcgcaca accttcataa atctcacaga ggacattgtg
      841 gccatgttga ccacaacaag ccacgacagc gtattgctgc gttccgatga tcccctgctc
      901 gacatgaact acatcaccag tttcaccggc atgaccgaca aatagtttta taaactgatc
      961 agtgaaggct attttcaaac tgggcggtac aaaagcgaag tggagcagtg gatgcagcag
     1021 cgaatagagg gagcgtgggg gtagcaggat gaaacgtgtt gtgatgccgg tacgctggta
     1081 ttgtgcaaaa tgccggcgct actattgtgg aaatcagccc tgtccctggt gctggcgata
     1141 ctccctgcag acattctccg gtcagccaat aggttgttgc tctttcctga tcgattcgtc
     1201 attccattct ttttgtttat aatgggcatt acactggtgt tggcgtgttt acctgcgtgt
     1261 ctgcaccggt tcagtaaaat tagaaaagtt cagagtcggg gaaaatccgg cgttttgttc
     1321 agcaggcggt ggatcactgg ccaaatctgt tggcatttca cttcacgttc cattcgacag
     1381 aaggaaacat ctacgggcaa cagattcatg cattctgcat ttccttttac caacagatac
     1441 acaaacgtat tactgcacgt actcacacga ccagtccatc atcgccgttg gtgttacact
     1501 ggctgctgga acaacatgga ggagcagtaa tacgatgcgt attgctcctc agccagacga
     1561 gtatttgtca tccgcgagcc agcgtcacgg ttgatgaaga atgttcgaaa ctggtggatt
     1621 tattgcaaca gatctggcca gtgataagca ctggcggaca atgccaagtg gaaaggtgtt
     1681 ttcaggttgc ccggggtgat acatcagagc aatacgttgc gttaaaaaca gatgcgttgt
     1741 cgtttgctgt gtcggatcac aaatcatccg gtgcagagta gcattctggc tgtattctgg
     1801 taaatcagcg tattatcgat atattatcgt actatcagaa accgtatcgt ctgttgatgg
     1861 ctagactggc ccccctgaat cttcagacaa ccaatatcac ttatttaagt gatagtctta
     1921 ctactagttt ttagactagt cattggagaa c
//
LOCUS       DAZGTR010000314         1916 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGTR010000314 DAZGTR010000000
VERSION     DAZGTR010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.316"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..600)
                     /gene="nleH1-2"
                     /locus_tag="ACHLCY_005216"
     CDS             complement(<1..600)
                     /gene="nleH1-2"
                     /locus_tag="ACHLCY_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase
                     NleH1-2"
                     /protein_id="HFP0372238.1"
                     /translation="MLSPSSINLGCSWNSLTRNLTSPDNRVLSSVRDAAVHSDSGTQV
                     TVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEAMLNTSPVSTTI
                     ERGSVHSNRPDLPPVDYAQPELPPADYTQSELPRVSNNKSPVPGNVIGKGGNAVVYED
                     MEDTTKVLKMFTISQSHEEVTSEVRCFNQYYGSGSAEKIY"
     gene            complement(707..778)
                     /locus_tag="ACHLCY_005217"
                     /pseudo
     CDS             complement(707..778)
                     /locus_tag="ACHLCY_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            772..1448
                     /locus_tag="ACHLCY_005218"
                     /pseudo
     CDS             772..1448
                     /locus_tag="ACHLCY_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1452..1831)
                     /locus_tag="ACHLCY_005219"
                     /pseudo
     CDS             complement(1452..1831)
                     /locus_tag="ACHLCY_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131658.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
BASE COUNT          580 a          469 c          382 g          485 t
ORIGIN      
        1 atatattttc tctgcactcc cggaaccata atactgattg aaacaacgaa cttcgcttgt
       61 cacctcttca tggctttgag atatagtaaa catcttcaac acttttgttg tatcttccat
      121 atcttcatac acgacagcat taccaccttt accaataaca ttacctggca cgggtgattt
      181 attgttgcta accctcggca actctgattg agtataatcc gctggaggta actccggctg
      241 cgcataatcc actggaggta aatcaggtct gttcgaatga acagagcctc tttctatagt
      301 cgtgctcact ggcgatgtat tcagcatagc ctcaattttt ctgctaatct ctcccttagg
      361 ccatcccaac ctgtgcaaca gattagtaaa acaaccacta tgactttctc ttgtaacgca
      421 aaacttatta tcagtgacca caacacgata tgttctgttg ccaaccgtta cttgcgtccc
      481 gctatcagag tgaacagcag catcccttac agaggataaa acacgattat caggcgaagt
      541 caggtttctg gttaaagaat tccatgaaca tcccaaattt atagaagagg gcgataacat
      601 acatttcaac cttcaaaata aacctatcta attattccta acagacatcc cccatacatg
      661 acaacaaaaa ccggagccgg actccggttt ttgtgaagct atcgggttac ttcatttcgc
      721 caatattttc ccacttcccg tcagcacgca ggatttgcag cggtcttact acacactgac
      781 ccgaggacga aaaaagagtt cgggctggga agagacagaa gagtagcaat ttcagaagca
      841 ttcacaccaa cattgagctt ttcagcgaaa gtgggcacga atcattgctg gacagggtta
      901 aaggcactga ctcaatcact cttcatacgt ggcttgatcg atatgaaaca atcctcagct
      961 agagggggag caagccgaaa acattactca actacgccag caaaatcagg gcaattcgaa
     1021 gaaaattgcc ggacaaaccg ctcactgaca tatcaacgaa agaggtggca gcaatgctaa
     1081 acacctacgt cgcagaaggt aaagcggttt ccgcaagagt aatcaggtca acccttgttg
     1141 acgtttttcg aggggcaata gccgaggggc atgtggcaac gaatccagta acaacaaccc
     1201 gtgcagcaaa gtcagaagta aggcgctcaa ggctgacagc taatgagtat gtcgctattt
     1261 accatgctgc tgagcacctc cccatctggc tgaggctgtc aatggattta gctgtcgtta
     1321 cagggcagag agtgggcgat ttgtgcagaa tgaagtggtc agacataaac gatggtcatc
     1381 ttcacattgg acagagtaaa acaggagcca aaattgccat tccgctggct ctaaccattg
     1441 acgcactcga cttcgggtct ctctagtgta attggacact gaatagactc tggcgggcac
     1501 tggaaatttc ctgaactaac aggaaactta cattgagata ttttgtcctg aatactctct
     1561 tgcgattgtg cttcacctga gtccgaaagc atactgagca tcctatcgcg aagagcttca
     1621 gggccgttat taaacccata aaggaataac tcagtaagca atcccccact actgccgctg
     1681 gatagaaaac gcccatcggt ttcattaaat acaatgctga cagtctcatt acctaatgta
     1741 aactcccctc gaccgctaga tgctacttga gctcgaagga cattcaatcc attaaaaatg
     1801 agcttgatct aatctccgaa tttaacggca ttataaaact ccctatttta atttaaactc
     1861 cagacttaaa tagctttaac aaacatctgc cttacatgac aacaaaaacc ggagcc
//
LOCUS       DAZGTR010000315         1902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGTR010000315 DAZGTR010000000
VERSION     DAZGTR010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.317"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..563
                     /locus_tag="ACHLCY_005220"
     CDS             <1..563
                     /locus_tag="ACHLCY_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0372239.1"
                     /translation="VWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQYFKL
                     QLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQAIET
                     LSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQ
                     DTNNSSFSYGDNRFEKSQGSNWTFQE"
     gene            577..817
                     /locus_tag="ACHLCY_005221"
                     /pseudo
     CDS             577..817
                     /locus_tag="ACHLCY_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            837..1168
                     /gene="sctI"
                     /locus_tag="ACHLCY_005222"
                     /pseudo
     CDS             837..1168
                     /gene="sctI"
                     /locus_tag="ACHLCY_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            1165..1899
                     /locus_tag="ACHLCY_005223"
     CDS             1165..1899
                     /locus_tag="ACHLCY_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP0372240.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
BASE COUNT          652 a          312 c          379 g          559 t
ORIGIN      
        1 cagtctgggt caaacaagca ctggaaaaaa atcaaccagg aaagccagta cgggtgataa
       61 atcctgatga tgaatcgata cgaatttttt catggcttgc tgataatttc cctgatttac
      121 aatattttaa acttcaacta ttagatgcca gtaatctaag attgacagtg agtaagcaac
      181 ggaacgctat cacacagcaa ctaatcgata atcttattaa aggattactg caaactatgc
      241 catatgccag caatataagt attgcggtat tagatgataa tgtattggaa agtcaggcta
      301 ttgaaacgtt gtcagcgata ggtctttctt atgaaaaata taaaacagct aacaatgtgt
      361 atttcaatat cattggtacg ttaagtgaca gtgaattaaa taaaattaat aactatgttg
      421 acgaatatta taaacaatgg ggtaaacaat atgtaagatt taatgtgaat ttgaaaaatc
      481 aggacacaaa taatagttca tttagctacg gagataaccg attcgagaag tctcaaggta
      541 gcaactggac atttcaggaa taattgagga ggacttatgg cagattggaa tggttatatt
      601 atggatatca gcaatcaatt tgatcagggc gttgatgatc tgaaccagca agttgaaaaa
      661 gcgttggagg atttagccaa ccaatccctc cgacacgaaa tttcttgccg aatatcaaag
      721 tgcattagct gagtatacat tatatcgaaa tgcgcaatct aacgttgtaa aagcgtataa
      781 agatcttgat tcagcaatca tacaaaactt ccgctaaact ggataaggat tttattatgt
      841 cagtaagcaa tatgccacct attgatagag ctgagcaatc aactgctcat gaaatacagc
      901 aagcgaaagt gattgattta aatgaccggg ttctcaatct tgacaacccg gatgataaaa
      961 tgatctccgc ttttgccaac tatgctgtac aaactgaaaa ttggcagcaa aatgcgttac
     1021 aagcattaag gtctgataaa gagggggtaa ctcctgaaaa attattagtc ttgcaggatc
     1081 atgtgcttaa ttataatgtc gaggtttcat tggtggaaca ttagcaagaa agattgtcgc
     1141 tgcagttgag acgttgaccc gctcatgaaa tatatatcat tattattgtt tatcctgctg
     1201 ttgtgcggct gcaagcagca ggaattattg aaccatcttg atcaacagca agctaatgat
     1261 gtccttgctg ttttacaaag acataatatt aatgctgaaa agaaggatca aggcaaaaca
     1321 ggattttcaa tatttgtcga accaacggat tttgcttctg cggtggattg gctaaaaatc
     1381 tacaatctac cgggtaagcc tgatattcaa atatcgcaga tgttccctgc agatgcactc
     1441 gtttcatctc cacgagcgga aaaagcaagg ctatattctg ctattgagca gcggttagag
     1501 caatctctca aaattatgga tggcattatt tcttcgagag tacatgttag ttacgatgtt
     1561 gataatggcg atagtggtaa aactgcttta ccaattcata tttctgtact tgcagtatat
     1621 gaaaaagata tcaacccaga aataaaaata aatgatatca agagatttat tgttaatagc
     1681 tctgcaagcg tacaatatga aaatatttct gttgtattgt caaaacgaag agacattatt
     1741 gagcaagcac caacatatga aattagcgaa cctgtttttg cttatgataa ggctatgccg
     1801 gtttctatct tacttgcatt aatatccgta gcgacttgct ggctgttatg gaagtatagg
     1861 gccattctaa ctaatttagt caggttgaaa attaagtaga ca
//
LOCUS       DAZGTR010000316         1857 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGTR010000316 DAZGTR010000000
VERSION     DAZGTR010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.318"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1210..1812
                     /gene="fimB"
                     /locus_tag="ACHLCY_005224"
     CDS             1210..1812
                     /gene="fimB"
                     /locus_tag="ACHLCY_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP0372241.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKSWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          560 a          334 c          391 g          572 t
ORIGIN      
        1 aagctatatc acttactcaa tccattttac ccagagtcat aaaatagaat gtctgttgat
       61 ctcattttca tattcctttc agactgaagt gcgttttcat cacccgatga gaaagaactg
      121 aagtgatgtt tgccatagat tgcgagatgc cctgtctcat tgactatctt ggtgcacctt
      181 tatacctgtt ataccagatc aaaaatcacg caatccatac aacaaaacca gatttgcaat
      241 tcgtgtcaca aaatatgtcg atatttttct aagaagaaga tgccatgtga agccagacga
      301 acacttgcgg aggccttcac aaaccaaaga ttgttgttta attatctgtt ttataagtaa
      361 tttatttaaa gtataaacaa ggaaagattt gcaagctaac tcagtgagcg tggtgaaaat
      421 cagtgtttac ccgccatcag gctgagcata attctcatca tgaaatatgt ttcctggttt
      481 gtggcttgta actggtcact tctgaagtcg atctggagag gcttgttgat gttggtgttt
      541 tcaggatgat gtttcactta gtttgtttgc cgtatcgccc ggcgaatggc tgtgattgag
      601 gaaggtttaa gtcgtagtga ccaaagctat atttaccaac gaatgtagat gaaaaaatca
      661 tctcctgcgt tccccatatc tccaggataa aaaggaatgt aacaatctca tggcgtaagc
      721 tgacgaatca gccaggaata atcgctaggg acctaagaat tagcatgata atagccacta
      781 agaaattact gcgctccatg aaatagccat tttgtggcaa atggagttga ctaataatgt
      841 catatgtgag acggctagtt gaacgaatat taaattttgc tgaatttttt atgttgattt
      901 tacttgttac agaacatatc acatgatata tagataagat tagttgcatt aatgatgagg
      961 gttattatta gattcgtatc cgattgataa atatataaag gtacatagca tgcaagagca
     1021 tggcgtttgt atggcaacgt tattataatt aactgttgct actccattta agttcactca
     1081 gaagaactgg tccacttacg ttagttatta agcaaacgtt cgcttttata aacataatca
     1141 ggataaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattataag
     1201 ggaaaaacga tgaagaataa ggctgataac aaaaaaagga acttcctgac ccatagtgaa
     1261 attgaatcac tccttaaagc agcaaatacc gggcctcatg cagcacgtaa ttattgtctg
     1321 actttgcttt gttttattca tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg
     1381 gatattgatc ttaaggcaaa gtgtatatat atccatcgat taaaaaaagg cttttcaaca
     1441 acgcatccgc tattgaacaa agaagttcag gctttaaaaa gctggttgag tatccgtact
     1501 tcgtacccgc atgctgagag cgagtgggta tttttatctc gtaagggaaa tccgctttct
     1561 cggcaacagt tttaccatat tatctcgact tccggtggta atgccgggtt gtcactggag
     1621 attcatccgc acatgttacg ccattcgtgt ggttttgctt tggcgaatat gggaatagat
     1681 acgcgactta tccaggatta tcttgggcat cgcaatattc gtcatactgt ctggtatacc
     1741 gccagcaatg cagggcgttt ttacggcatc tgggatagag ccagaggacg acagcgtcac
     1801 gctgttttat agagatagtt gagataccag ggatggtgtt aaaaactaca ttattct
//
LOCUS       DAZGTR010000317         1837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGTR010000317 DAZGTR010000000
VERSION     DAZGTR010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.319"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1130
                     /locus_tag="ACHLCY_005225"
                     /pseudo
     CDS             1..1130
                     /locus_tag="ACHLCY_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          490 a          396 c          479 g          472 t
ORIGIN      
        1 cttgttgccc ggcaacacgg tgtggcagcc agccagttat ttctctggcg caagcaatac
       61 caggagggaa gtcttactgc tgtggccgca ggagagcagg tcgttcctgc ctctgaactt
      121 gctgctgcca tgaagcagat taaagaactc cagcgcctgc tcggaaaaaa aacgatggaa
      181 aatgaactcc ttaaagaagc cgttgaatat gggcgtgcaa aaaagtggat agcgcacgcg
      241 cccttattgc ccggggatga ggagtaaacg tcgtcagtcg ttgtcgccgg gtgtcgcatg
      301 agcagttgca catcattctc agacgaagtg atgactggaa ggatggccgc cgcagtcttc
      361 acactgatga tacggatgtg cttctccgga tacatcatat catcggagag ttgcccacgt
      421 atggttatcg tcgggtatgg gcgctgcttc gcagacaggc agaacttgat ggtatgtctg
      481 cgatcaatgc caaacaagtt taccggatca tgcgccagaa tgcgctgttg cttgagcgaa
      541 aacccgctgt accgccatcg aaacaggcac ataccggcag agtggccgtg aaagaaagta
      601 atcagcgatg gtgctctgac ggggtcgagt tccgctgtga taacggagag agactgcgtg
      661 tcacattcgc gctggactgc tgtgaccgtg aggcactgca ctgggcagtc actacgggcg
      721 gcttcaacag tgaaacagta caggacgtca tgctgggagc ggtggaacgc cgcttcggca
      781 acgagcttcc ggcgtctcca gtagagtggc tgacggataa tggttcatgc taccggccta
      841 atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc gaagaacacg gcggtgcgga
      901 gtccggagag taacggcata gcagagagct tcgtgaaaac gataaagcgt gactacatca
      961 gtgtcatgcc caaaccagac gggttaacgg cagcaaagaa ccttgcagag gcattcgagc
     1021 attataacga aaggcatccg catagtgcac tgggttatcg ctcgccacgg gaatatctac
     1081 ggcagcaagc cagtaatggg ttaagtgata acaggtgtct ggaaatatag gggcaaatcc
     1141 agcgacaaag aacagggtta atgaatctgt aaccagtccc accttagcgc tatgcacttt
     1201 tgaatcacag atcttgctaa tcgtttgtcc gactttaaca gagttaatga cggcgatgat
     1261 ctgatgccaa gtaaaacgga cttgatgtat ggcagatcct ctttcgccga atggatatgg
     1321 gcaatacttc ttaatacgaa tggtacggtt atgcgggaag agactaacgc ggttacttgt
     1381 tcagctattt gtcccttgtt cctttttata atgcacccgt tccaggttag tgctggctgt
     1441 agcttatgtc cgggagacag ctaatagata tatatactcg tcatacttca agttgcatgt
     1501 gctgcgactg cgttcgctta ccccaatcac ttacttatgt aagctcctgg ggattcactc
     1561 gcttgccgcc ttcctgtaac tcgaattaag tagagtatag tgaaacggtt cagcttggtt
     1621 tttattctgt tttatttgtt tatgcaatga gatctatctt ataaagagaa acgcttaatt
     1681 aaatggaaat gcaattatta aagtcgtttg ccaacgagat tgtttttctt ctatatcatt
     1741 gattttaaat ggattttaaa aatatatgat ttttttgttg acatttaatg ataatgtatt
     1801 ttacacatta gaaaacagag aataataaca ttttaag
//
LOCUS       DAZGTR010000318         1837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGTR010000318 DAZGTR010000000
VERSION     DAZGTR010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.320"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..403
                     /locus_tag="ACHLCY_005226"
     CDS             <1..403
                     /locus_tag="ACHLCY_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372242.1"
                     /translation="GERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETGVAGPVGA
                     TGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGALITGLTFLDPKDATRVQGF
                     FQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            405..674
                     /locus_tag="ACHLCY_005227"
     CDS             405..674
                     /locus_tag="ACHLCY_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372243.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDTDKKLIQCVVRPLQILRADGTWENIGGMK"
     gene            780..1661
                     /gene="nleH"
                     /locus_tag="ACHLCY_005228"
     CDS             780..1661
                     /gene="nleH"
                     /locus_tag="ACHLCY_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector kinase NleH"
                     /protein_id="HFP0372244.1"
                     /translation="MLSPSSVNLGCSWNSLTRNLTSPDSRILSSVRDAAASSDNGAQV
                     KVGNRTYRVVVTDNKFCVTRESHSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRAM
                     ERGAVHSNRPDLPPVDYAPPELPSVDYNSLPVPGNVIGKGGNAVVYEDAEDATKVLKM
                     FTTSQSNEEVTNEVRCFNQYYGAGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQ
                     AEHAIYDMFDRLEQKGILFIDTTETNVLYDRTRNEFNPIDISSYNISERSWSENQIMQ
                     SYHGGKQDLISVVLSKI"
     gene            complement(1636..>1837)
                     /locus_tag="ACHLCY_005229"
     CDS             complement(1636..>1837)
                     /locus_tag="ACHLCY_005229"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372245.1"
                     /translation="KRTSGMGIMSCLKFFWCLYAIRQSLSGIVHFCDAAWIASHTTFY
                     QLHPCNKSTTLDKITKFYLIPH"
BASE COUNT          536 a          331 c          514 g          456 t
ORIGIN      
        1 gggagagcgg ggagagaccg gtctgacggg aaatgcaggt ccacagggtc caaagggaga
       61 taccggtgcg gcaggcccgg caggcccaca gggaccgaaa ggagaaacag gtgtggctgg
      121 cccggtaggg gcaaccggac ctcagggacc aaagggcgac ccgggggaga cgcaaatacg
      181 gttccgtctg gggccgggaa acattattga gacaaacagc aatggctggt tcccggatac
      241 agatggcgca ctcatcaccg gactgacctt tcttgacccc aaagatgcca cacgggttca
      301 ggggtttttt cagcatttgc aggtcaggtt tggtgacggg ccgtggcagg atgtcaaggg
      361 gctggatgaa gtgggcagtg atacaggcag aacaggagaa tgacatgaat atactaaaaa
      421 aacttatgca gcgtctgtgt ggttgcggaa agcatgatgg ccgtgaacac gtgcagtcgc
      481 ttacagcaca actgcgactg gggccggcag acatcctgga gtcagatgag aatggcatta
      541 ttccggagca ggacagggta atcacacagg tggtgatact ggatacagat aaaaagctga
      601 tacagtgtgt ggtaagaccg ctgcaaatcc tgcgtgctga cgggacgtgg gaaaatattg
      661 gcgggatgaa gtaacccgac agcttcacaa aaccggagtc cggctccggt ttttgttgtc
      721 atgtcatggt gatgtttgtt aagaaagtaa agattgattc attttgaagg ttgaaatgta
      781 tgctatcacc atcttctgta aatttggggt gttcatggaa ttctttaacc agaaacctga
      841 cttctcctga cagtcgtatt ttatcctctg taagggatgc agctgctagc tcggataatg
      901 gggcgcaagt aaaggtgggc aacagaacat atcgtgttgt tgtcactgat aataagtttt
      961 gcgttacaag agaaagtcat agtggttgct ttactaatct gttgcacagg ctgggatggc
     1021 ctaagggaga gattagcagg aaaattgagg tgatgctgaa ttcatcgcca gtgaataggg
     1081 ctatggaaag aggtgctgtt cattcgaata gacctgattt acctcctgtt gattatgcac
     1141 cgccagagtt accgagtgtg gactataaca gcttgcctgt gcctggtaat gttattggca
     1201 aagggggtaa cgctgtagta tatgaagatg ctgaggatgc aacaaaagtc ctgaagatgt
     1261 ttactacatc tcaaagcaat gaagaggtaa caaacgaagt tcgttgcttc aaccaatatt
     1321 atggtgccgg gagtgcagaa aaaatatatg gcgataatgg cgatattatt ggtattagaa
     1381 tggataaaat aaatggagaa tcgcttttaa atatttcgtc cttgccggca caggctgagc
     1441 atgccattta tgatatgttt gacaggcttg agcaaaaagg aattcttttt atcgatacaa
     1501 cagagacaaa tgtcttatat gatcgcacga gaaatgaatt taatccaata gatatatcat
     1561 cttataatat ttctgaacgt tcatggagtg aaaatcaaat aatgcaatct tatcatggag
     1621 gaaagcaaga tcttattagt gtggtattaa gtaaaattta gtaattttat ccagtgtagt
     1681 ggatttgttg catggatgga gttggtaaaa agtggtgtgg ctggcaatcc aggccgcgtc
     1741 acagaaatgg acaatgccgc tgagagactg gcgaatggca tacaggcacc agaaaaattt
     1801 cagacatgac ataatgccca tgccagaagt tcttttt
//
LOCUS       DAZGTR010000319         1815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGTR010000319 DAZGTR010000000
VERSION     DAZGTR010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.321"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1352
                     /gene="rfbK"
                     /locus_tag="ACHLCY_005230"
                     /gene_synonym="manB"
     CDS             <1..1352
                     /gene="rfbK"
                     /locus_tag="ACHLCY_005230"
                     /gene_synonym="manB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O111 family phosphomannomutase RfbK"
                     /protein_id="HFP0372246.1"
                     /translation="KAYDIRGRLGAELNDEIAYRIGRAYGEFFKPQTVVVGGDARLTS
                     ESLKKSLSNGLCDAGVNVLDLGMCGTEEIYFSTWYLGIDGGIEVTASHNPIDYNGMKL
                     VTKGARPISSDTGLKDIQQLVESNNFEELNLEKKGNITKYSTRDAYINHLMGYANLQK
                     IKKIKIVVNSGNGAAGPVIDAIEECFLRNNIPIQFVKINNTPDGNFPHGIPNPLLPEC
                     REDTSSAVIRHSADFGIAFDGDFDRCFFFDENGQFIEGYYIVGLLAEVFLGKYPNAKI
                     IHDPRLIWNTIDIVESHGGIPIMTKTGHAYIKQRMREEDAVYGGEMSAHHYFKDFAYC
                     DSGMIPWILICELLSLTNKKLGELVCGCINDWPASGEINCTLDNPQNEIDKLFNRYKD
                     SALAVDYTDGLTMEFSDWRFNVRCSNTEPVVRLNVESRNNAILMQEKTEEILNFISK"
     gene            1389..>1815
                     /locus_tag="ACHLCY_005231"
     CDS             1389..>1815
                     /locus_tag="ACHLCY_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0372247.1"
                     /translation="MKVLLTGSTGMVGKNILEHDSASKYNILTPTSSDLNLLDKNEIE
                     KFMLINMPDCIIHAAGLVGGIHANISRPFDFLEKNLQMGLNLVSVAKKLGIKKVLNLG
                     SSCMYPKNFEEAIPEKALLTGELEETNEGYAIAKIAVAKA"
BASE COUNT          620 a          256 c          388 g          551 t
ORIGIN      
        1 tcaaagcata tgatatacgt gggcgtcttg gtgctgaatt gaatgatgaa atagcatata
       61 gaattggtcg cgcttatggt gagtttttta aacctcaaac tgtagttgtg ggaggagatg
      121 ctcgcttaac aagtgagagt ttaaagaaat cactctcaaa tgggctatgt gatgcaggcg
      181 taaatgtctt agatcttgga atgtgtggta ctgaagagat atatttttcc acttggtatt
      241 taggaattga tggtggaatc gaggtaactg caagccataa tccaattgat tataatggaa
      301 tgaaattagt aaccaaaggt gctcgaccaa tcagcagtga cacaggtctc aaagatatac
      361 aacaattagt agagagtaat aattttgaag agctcaacct agaaaaaaaa gggaatatta
      421 ccaaatattc cacccgagat gcctacataa atcatttgat gggctatgct aatctgcaaa
      481 aaataaaaaa aatcaaaata gttgtgaatt ctgggaatgg tgcagctggt cctgttattg
      541 atgctattga ggaatgcttt ttacggaaca atattccgat tcagtttgta aaaataaata
      601 atacacccga tggtaatttt ccacatggta tccctaatcc attactacct gagtgcagag
      661 aagataccag cagtgcggtt ataagacata gtgctgattt tggtattgca tttgatggtg
      721 attttgatag gtgttttttc tttgatgaaa atggacaatt tattgaagga tactacattg
      781 ttggtttatt agcggaagtt tttttaggga aatatccaaa cgcaaaaatc attcatgatc
      841 ctcgccttat atggaatact attgatatcg tagaaagtca tggtggtata cctataatga
      901 ctaaaaccgg tcatgcttac attaagcaaa gaatgcgtga agaggatgcc gtatatggcg
      961 gcgaaatgag tgcgcatcat tattttaaag attttgcata ctgcgatagt ggaatgattc
     1021 cttggatttt aatttgtgaa cttttgagtc tgacaaataa aaaattaggt gaactggttt
     1081 gtggttgtat aaacgactgg ccggcaagtg gagaaataaa ctgtacacta gacaatccgc
     1141 aaaatgaaat agataaatta tttaatcgtt acaaagatag tgccttagct gttgattaca
     1201 ctgatggatt aactatggag ttctctgatt ggcgttttaa tgttagatgc tcaaatacag
     1261 aacctgtagt acgattgaat gtagaatcta ggaataatgc tattcttatg caggaaaaaa
     1321 cagaagaaat tctgaatttt atatcaaaat aaatttgcac ctgagttcat aatgggaaca
     1381 agaaatatat gaaagtactt ctgactggct caactggcat ggttggtaag aatatattag
     1441 agcatgatag tgcaagtaaa tataatatac ttactccaac cagctctgat ttgaatttat
     1501 tagataaaaa tgaaatagaa aaattcatgc ttatcaacat gccagactgt attatacatg
     1561 cagcgggatt agttggaggc attcatgcaa atataagcag gccgtttgat tttctggaaa
     1621 aaaatttgca gatgggttta aatttagttt ccgtcgcaaa aaaactaggt atcaagaaag
     1681 tgcttaactt gggtagttca tgcatgtacc ccaaaaactt tgaagaggct attcctgaga
     1741 aagctctgtt aactggtgag ctagaagaaa ctaatgaggg atatgctatt gcgaaaattg
     1801 ctgtagcaaa agcat
//
LOCUS       DAZGTR010000320         1810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGTR010000320 DAZGTR010000000
VERSION     DAZGTR010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.322"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1327)
                     /locus_tag="ACHLCY_005232"
     CDS             complement(<1..1327)
                     /locus_tag="ACHLCY_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFP0372248.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIAPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQ"
     gene            complement(1416..>1810)
                     /locus_tag="ACHLCY_005233"
     CDS             complement(1416..>1810)
                     /locus_tag="ACHLCY_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372249.1"
                     /translation="LHFLKNEICEALNRHVRVIVFSFNKLITPDPRVELYYRFNHEEE
                     YYPSHRFMLVVDMKTVMVFSQRAESLGLYSNNRLLINIIAEHIHSDIYLTKYQQQYPD
                     ANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          570 a          352 c          321 g          567 t
ORIGIN      
        1 attgtgtaat tttaattgca acattattaa atgtgccata aaactcacac gggccataaa
       61 ataatgcagt aaaaccattg ttattttctt gaaacatatt ttcgacaaaa gccggtatgg
      121 ttcttgctgt attcggtacg cagcagacag cagcctcctc atgagtaggg gagtatttat
      181 atctggtatt aaactgatta ctgtccggat gtttcctttc atttgcttca taacaattat
      241 cagtcttaca gtacattatc gcgtgaagta tgggatgctg catgccaaaa ctagcattat
      301 agaatagcca ctcagcatca tcggccagac caatattagt ggtcagtttt attaacataa
      361 cataagtgct aaatagttca tgaacagaac aatattcata gcctgtataa gtggcattag
      421 ctgtgcgtcc tgtaatccac tcatcaccaa tgggagcgcc agatggtgta agataatatt
      481 tcaactggga taaaaaaata tctaatgcag tttgatactg cggccgttgg ctcgctgcga
      541 tggctaatgc cctaatatgc tcatacgtat gtacgccatg acctttaaga aggtaaccag
      601 gggctatgac atttttatat tgcagatctt gctccgacac gcatcctgcg gaataatctt
      661 catacagcca caagcaatat gagagatatt caatgttatt ggttatttca taaaggcggt
      721 agaaaacatc tgttattgtt aatccgtggc tatgtccagc aaaaccctgt tccacaacga
      781 aaggatgtga cgtttcttta gggtagccag ccataatgca ttgtgcggct tgggtaagag
      841 aagttaataa actctcatta cgggtggact cataacatcc aagcacaaaa cgcaaggcag
      901 tagcttgtgc ccagaactct ccattttctg ctttacagtt aaattttaat gatgaatcat
      961 aaattccaat atagccatca tcatcttgcg tatcgataag ttccaaacat aaatcgctcg
     1021 ctttttcctg ccattcttta ttatctaaca agtaggcact gcggcaatat ccatccttcc
     1081 aattcgattg agtttcacta tcccaccaat aatattgcat catgttatca tcgggattat
     1141 tctgtttttt aataacgcca tcttcagcag atacagtgcg tggagttata cgatctcttc
     1201 catatatttt atgctccgta ataagatggg gaactaattt atccagttct cctatatgac
     1261 cgttcatata ccattccatc ttattcttta accaaccagt gggctttata tcgttaaaaa
     1321 aaatcataaa tatacctttg cataaaaaag tttagacgtt ataaaagaca aatagattgt
     1381 gatcgcctac agacgatcac aatgaaagga aaatattaac ggtttttgct atcactcaaa
     1441 tgtttttcat cctgcaccat atcattgcca gattcatgca aagtatttat taatgcattg
     1501 gcatctggat attgttgctg atacttggtt agatatatat cactatggat atgttcggca
     1561 attatattga taagcaagcg attattcgag tataacccca gtgattcagc tcgttgtgaa
     1621 aaaaccatta ctgtcttcat atcaacaaca agcataaaac ggtgagaagg gtaatattct
     1681 tcttcatggt taaagcggta atacagttct acacgtgggt cgggagtaat aagtttatta
     1741 aatgaaaaaa caataactcg aacatgtcga ttcagagcct cacaaatttc atttttcaaa
     1801 aaatgtaagt
//
LOCUS       DAZGTR010000321         1801 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGTR010000321 DAZGTR010000000
VERSION     DAZGTR010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.323"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            123..374
                     /locus_tag="ACHLCY_005234"
     CDS             123..374
                     /locus_tag="ACHLCY_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0372250.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            409..1689
                     /locus_tag="ACHLCY_005235"
     CDS             409..1689
                     /locus_tag="ACHLCY_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFP0372251.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKENEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLKEYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAIKN"
BASE COUNT          589 a          367 c          402 g          443 t
ORIGIN      
        1 acgtttcagt tatctggcaa aaactgatcc cgagtcaggc tgattcttaa tgttcaactg
       61 tcgccggttg tgactggtat tctgcaaccg gcgctcgtct gatgtaagag ataaagaaat
      121 cgatgagcga agtaatcatg attgtctctc ccggcaaatg ggtatccgaa gagcagttaa
      181 ttgcgctgaa aggaataaaa aaaggtacgt taaaaaaggc ccgggaaaaa tcgtttatgg
      241 aaggaaggga atataagcat gtcgctcatg acggtatgcc atgggataac agtccatgct
      301 tttacaacct ggaagaaatt gatcgctgga ttgagcgcca ggcatcagcg agaccaagac
      361 gtcatcttac ttgactaaaa gccacactaa ctaatgagag aagttgaaat gaaatatccg
      421 acaggcgtgg aaaaccatgg agggaaatta cgtatctggt ttgtttataa aggcgtaaga
      481 gtcagggaaa atctgggggt tcctgacaca gcaaaaaaca ggcgcgttgc aggtgaacta
      541 cgctcctctg tttgttacgc aataaaaact ggtgttttcg actatgcaaa acagtttccc
      601 tcctcacgca atctggaaaa atttggtgag gcccgacaag atttaaccat aaaagaactg
      661 gctgaaaaat ttctggcact gaaagaaaat gaagtcgcca aaacatcact caacacatac
      721 cgtgccgtca tcaaaaatat cctgagcata atcggtgaaa aaaatcttgc ctcatcgatt
      781 aataaagaaa aattactgga ggttcgtaaa gagctactga ctggatacca gatccccaaa
      841 agtaactata ttgttacaca accagggaga tcggctgtaa ctgtaaataa ttacatgaca
      901 aatcttaacg ccgtgttcca gtttggtgtt gataacggtt acctggcaga taatccgttt
      961 aaggggatct cgccattaaa ggaatcaaga accattccgg atcctctttc gcgggaggaa
     1021 tttatccgtc ttatcgatgc gtgcagaaat cagcaagcaa aaaatttatg gtgtgtttct
     1081 gtttatactg gcgttcgccc tggtgagctg tgtgcacttg gatgggagga catagatctg
     1141 aaaaatggaa caatgatgat caggagaaat ttagcaaaag accgtttcac ggtaccaaaa
     1201 acacaggcgg gaaccaatcg ggtcattcat cttattaagc cagcaatcga cgctctccgg
     1261 agtcagatga cattaacgag actgagtaaa gagcatatca ttgatgttca cctcaaagag
     1321 tatggcagaa cagaaaaaca aaaatgcacc tttgtttttc aacctgaagt gtcagcgaga
     1381 gtaaaaaatt atggtgacca ttttaccgtt gactcaataa ggcagatgtg ggacgcagcg
     1441 ataaaacgtg ccggactccg ccatcgaaaa tcatatcagt cgagacatac ttatgcctgc
     1501 tggtcgctga cagctggtgc taacccggca tttatagcaa accagatggg ccatgcagat
     1561 gcgcaaatgg tatttcaggt atacggaaaa tggatgtctg aaaacaataa tgcacaggta
     1621 gctttgttaa atacacagtt aagcgagttt gccccaacca tgccccataa cgaagcaata
     1681 aaaaattaat ttaatattta tcaaatagtt aacacgcatg actcttgaaa tccataaatt
     1741 caagcgcagt gcccagccat cccgatactg ctgctttcac caaatcctta gtgcttcttt
     1801 c
//
LOCUS       DAZGTR010000322         1792 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGTR010000322 DAZGTR010000000
VERSION     DAZGTR010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.324"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1113
                     /locus_tag="ACHLCY_005236"
     CDS             <1..1113
                     /locus_tag="ACHLCY_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0372252.1"
                     /translation="SVNLVGKSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGLWEL
                     IDNSQLADKMAEMKTSITETRNEITQRVSKTLEDQSATIQQIQRVQKDTNDDLAALYM
                     LKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQ
                     LFMNDVFLKRLFAVSITSSGNPPTFSLTPEGKLTARNADISGAITANTGTLNNVTINE
                     NCVIRGKLSANQIEGDLVKTVGKAFPRNNSYASGTVTVTVYDDQGFDRQIIIPPVLFR
                     GTKHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHEPATDVSRVFSSVIDMPAGRGHVTL
                     TFNVSSAGANNWTPTTYISDLLVVVMKKSTAGISIS"
     gene            1182..>1792
                     /locus_tag="ACHLCY_005237"
     CDS             1182..>1792
                     /locus_tag="ACHLCY_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0372253.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMADPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSASGDWRTDGFIVGIG"
BASE COUNT          445 a          428 c          533 g          386 t
ORIGIN      
        1 agcgtcaacc tggtggggaa atctgctttt gtggaagcca gtggccgggc cagcaatgat
       61 gcagaagggt atctggggct gtttcgggaa aaaataggaa aactgcatct ggctcagggg
      121 ctgtgggagc tgatagacaa cagccagctt gcggataaga tggcggagat gaagacctcc
      181 atcaccgaaa cccgcaatga aatcacacag agggtcagta aaacgctgga ggaccagagc
      241 gccaccatac agcagataca gcgcgtgcag aaggacacaa atgatgacct tgctgcactt
      301 tacatgctga aggtacagaa aacaaaaaat ggcataccct atgttgccgg tattggagcg
      361 gggattgagg atactgatgg ccagcccctg agcaacatac tgctgctggc tgaccgtatc
      421 gcgatgataa atccggagag cggcaacagc acgccgttat ttgtggcgca ggggaatcag
      481 ctgttcatga acgacgtgtt cctgaagcga ctgtttgcgg tgagtatcac gtcatccggc
      541 aaccccccga cgttttccct gacgccggaa gggaagctga cagccaggaa cgcggatatc
      601 agcggagcaa ttaccgcgaa taccggcacg ctcaataatg tcaccattaa cgagaactgt
      661 gtcatcagag ggaaactgtc tgcaaaccag attgaaggcg acctggtgaa gacggtgggg
      721 aaagcctttc cccggaataa cagttatgcc agcgggacgg taaccgtcac agtttacgat
      781 gaccagggct tcgaccggca gatcatcatt cccccggtgc tgtttcgcgg gacgaaacac
      841 cagaatttca acagcccgaa tcagcagtcg tactggtatt ccacctgtaa gctgcaggtg
      901 ctgaagaacg gggttgagat tttccatgaa ccggcaacgg atgtcagccg ggtgttctca
      961 tcggtgatag atatgccggc agggcggggt catgtcaccc tgacgtttaa tgtgtcgtcg
     1021 gccggtgcga acaactggac gccgacaacg tacatcagtg atttactggt tgtggtcatg
     1081 aaaaaatcca cggcagggat cagtatcagc tgacggttta ttaacccgga cgggcacctc
     1141 aggaggtgcc ttttttattg actgaaaaca aagaggtaat catgcggcat ttatacgcaa
     1201 cgatattatt gtttactacc ctgctggcag gaattgcctt tcctgcacag gctgaaagcg
     1261 gacacggtgc attttccgtg ggatatgctc aggttcaccc gggcggcgta ccggcattgt
     1321 ccggtaccgg tgcgcgtgca ggtgatttaa aagggattaa tgtgaaatac cgttatgagt
     1381 tcacggatca cctgggcggc attgtcgcgc tgagttatgc atcggtgaag aaaagtgaca
     1441 cgatgaagac gggtgaaaat accttccatt atgaaagcct gcgcggtcgt tatgtcagtc
     1501 tgatggccga ccctgtctgg cagctcagtg agcgggtcag tctctatggc atggccggga
     1561 tggcgtacac ccgctggtct gacagtgttc aggattaccg gcgtgatgaa gtgaaaccgg
     1621 ggtatgtgaa ggagaccacc accgccagtg atggccatac tgcgcgtcat ctgtcgccgg
     1681 cctggaatgc cgggattcag ttcagtcccg tagagacggt ggttattgac cttgcttatg
     1741 aaggttccgc cagtggcgac tggcgcactg acggcttcat cgtgggtatc gg
//
LOCUS       DAZGTR010000323         1785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGTR010000323 DAZGTR010000000
VERSION     DAZGTR010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.326"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            7..222
                     /locus_tag="ACHLCY_005238"
     CDS             7..222
                     /locus_tag="ACHLCY_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0372254.1"
                     /translation="MNQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            226..781
                     /locus_tag="ACHLCY_005239"
                     /pseudo
     CDS             226..781
                     /locus_tag="ACHLCY_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            818..1351
                     /locus_tag="ACHLCY_005240"
     CDS             818..1351
                     /locus_tag="ACHLCY_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0372255.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1650..>1785
                     /locus_tag="ACHLCY_005241"
     CDS             1650..>1785
                     /locus_tag="ACHLCY_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0372256.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKAQRDKKVSEL
                     E"
BASE COUNT          468 a          370 c          520 g          427 t
ORIGIN      
        1 cggggtatga accagatgga aaaaatcaca acaggtgtgt catacaccac gtcagcggtg
       61 ggaacgggct actggttcct gcagttgctg gacagggttt ccccgtctca gtgggcggca
      121 ataggcgtgc tggggagtct gctgtttggt ctgctgacgt acctgacgaa cctgtatttc
      181 aaaatcagag aggacaggcg taaggcggca cggggagagt aggtgatgaa ccatgaagaa
      241 atgaatcagc gcttcagtcg tctggaaaat gaaattgctg aactgaataa aaaactgtcg
      301 gcgctgatgc cttctgaaga tgaaaaaaaa cgccgcgatg agcagtttgc tgcgtttgac
      361 gattattgtc ggaaagtgat gagcagaaat ctcgcagagt gtttcagtat tcataatgat
      421 aattgcagtg agctggaatg agtgtaaccg gccatccttt gttgtatccg gtgatgacgg
      481 gaaaatcacc atatcagaaa atggaaaagt aacactgcca tcgcaccagc acagtgagac
      541 gctcattgaa tttgccattg attacctgaa gaacaataaa aaacaggggc tgatgcagcg
      601 cattggtcgt tgcatggggt atctgcaggt agccgctgag attgaagcgc tggccagtgg
      661 tgctgataag gatgcaattg tgcgggaggc tcttcttcgt aattttaata ctccaccctt
      721 taaaaaagtg ccggcttact ggcttcatcc cggactgact tatctgaaag tgcgtattta
      781 gtgggcctgg gacagcggct gaatatttaa tatatccatg aacgcaaaaa tcagatacgg
      841 cctgtcggct gccgttctgg cgctgattgg tgcaggggcg tctgcgcctg aaatcctcga
      901 ccagtttctg gatgaaaagg aaggtaacca caccacggca taccgtgatg gtgcgggtat
      961 ctggaccatc tgccgtggag ccacccgggt ggatggtaag cctgttattc ctggcatgaa
     1021 gctgtcgaag gggaaatgcg accgggttaa cgccattgag cgtgataagg cgctggcatg
     1081 ggtggagaaa aacatcaaag tgccactgac tgaaccccag aaagcgggga tcgcgtcatt
     1141 ctgcccgtat aacattggcc ccggtaagtg tttcccgtcg acgttttata aacgaattaa
     1201 tgcaggcgat cgcaggggag cgtgtgaagc gattcgctgg tggattaagg acggaggcag
     1261 agactgccgt atccgctcaa ataattgcta tggtcaggta tcccgtcgtg accaggagag
     1321 cgcgctggcg tgctggggta tcgacagata agcagaatat tttgctgaaa aatgacgttg
     1381 gccaacgcgg acggataaca cgaaatcctg cgaactggca aaacctgagt gaataaaagt
     1441 aaaaaccccg tttgttagca gcaggtgggg ttttgtgttt cctgactccg gaaaagtcaa
     1501 aggagaaagt gtgtttgatt ttagcaaact gattcgggag attcgagtga tggctgaaaa
     1561 attatccacc tggaagttca ttcttatctg gctggtgttt gtgattatgg cctccggtta
     1621 tttcatcggt cagatacgct ggtggtgaaa tgaaccgcgt actgtgcgtg gtcatcattg
     1681 ccctgctggt ggcctgtggt gcgcttagtc tggggctgaa tcattaccgt gataacgcca
     1741 taacctacaa agcgcagcgc gataaaaaag tcagtgagct ggagc
//
LOCUS       DAZGTR010000324         1779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGTR010000324 DAZGTR010000000
VERSION     DAZGTR010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.327"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(289..516)
                     /locus_tag="ACHLCY_005242"
     CDS             complement(289..516)
                     /locus_tag="ACHLCY_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0372257.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            558..923
                     /locus_tag="ACHLCY_005243"
     CDS             558..923
                     /locus_tag="ACHLCY_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0372258.1"
                     /translation="MAVLHTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRRIWRR
                     DAGHCCLCRRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSASEAASGMPD
                     PTLPELPDGTLRADGITGL"
     gene            1213..1776
                     /locus_tag="ACHLCY_005244"
     CDS             1213..1776
                     /locus_tag="ACHLCY_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0372259.1"
                     /translation="MLTTQKRKFALALMSGKNKTASALAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEPEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEESG"
BASE COUNT          448 a          403 c          493 g          435 t
ORIGIN      
        1 gcgagagtgt gcggggataa tcaaaaacga tacacaccgg ggtttaccgc gtaaacggag
       61 cgcggcgttc tcccctcatg gtcgcccgtc cggtgcgatg gtggaagaaa ctggaatctg
      121 ttcaataaaa aaactgccgt gttggagtca cagcagtaat gtactgattg ggtagaagat
      181 tattattgtt atgctttatt tttattctat atggctgatt atttcaattc ggaattaata
      241 cagctaatgt ctgtgagttt ttataaattc agcaatataa agaaatagtt atatgaacag
      301 ccatcgcaga gcatactgtg tatcattctt ttttatagtc aactgacggg catattttat
      361 gtctgctgcc agctcccggc ggcaagattc aatgacccac gcagaaaaat tttctgaacc
      421 tttctggtca agagcgatgt taatttgttc aatcatctgg tttggaaatc ggatgttgcg
      481 ggttgttgtt ctgcggggcc ggtttttcga tgacattttc tttcctctgg tgacaagcta
      541 tatggcgagg atttttcatg gctgtgcttc atacgttacc gggcagaatc aaaactctga
      601 acacccggcg ggtgaatatt ctgaagggtg aacagcgtcg tgtcagtggc agtgcccggg
      661 tttccctcaa acgtcgtatc tggcggaggg atgccggaca ctgttgtctc tgtagacgcg
      721 tggttgacct ctgtgacagt gaactcgatc accgcattgc acttcagttc ggtggtggta
      781 atgaggagac gaacctctgg accctctgta ctgaatgcca tcgccagaag tcagcgagtg
      841 aagcggcgag tggtatgcct gatccgacgt tgcctgagct tcctgatggc acgctcaggg
      901 ccgacggaat cactggcctg tgaccagacc cgggggggat catccggcga aaaaaaacga
      961 tcgccccgga caccgccccc cgtctcatgc agagaaaaaa ttcctgtttc aggccagtta
     1021 acatgttaac tggctgcccg ggcatttttt cggtttttat ctttattatt cagtttgttg
     1081 tgcgaaaaaa atgttaactg gctttttcag caaatgttaa ccaggcagca gttaacattt
     1141 gcggcatgag acgccgggaa aaatgggctg aaccataccc ggctgagtgc gttctggacc
     1201 cgggaggagg ctgtgctgac aacgcaaaaa cgaaaatttg cgctggcgct catgtccggg
     1261 aaaaacaaaa cagcgtcagc ccttgccgcc ggttattcgg cgaagacagc cagggttaaa
     1321 ggctcgcagc tggcaaaaga tccggaggtg cttgcgttta tagcccgtaa acagtgcgag
     1381 acggtggagg tggatgaggt tcctgtttac cggcagaaaa aatcagagcc ggaggataaa
     1441 ccccgtcgcc gtgaggcggc tgcaatacca cagccggacg aaacaaatcc ggagatgcca
     1501 ccgcccgtgg tgatatctcc tggtattgag tatatggagg acggtcttcc cgatccggtg
     1561 aaagcgatgg ggcgtcttct ggtggagaac attaataccg accccaggct ggcgctggat
     1621 gcggcttata agctggcgca attcacgcac cacaaaaaag gggatgccgg taaaaaatcg
     1681 gcaaaaggtg acgcggcgaa aaaagcggct aaccgttttg cggtgccacc accaccccgc
     1741 ctggtggtga ataatgataa tgaggaaagc ggatgatac
//
LOCUS       DAZGTR010000325         1777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGTR010000325 DAZGTR010000000
VERSION     DAZGTR010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.328"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..229
                     /locus_tag="ACHLCY_005245"
     CDS             <1..229
                     /locus_tag="ACHLCY_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372260.1"
                     /translation="EKILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEEKVE
                     NIFMLYGADKNNKLRRRLIYDALGIIQSQQE"
     gene            complement(416..1618)
                     /locus_tag="ACHLCY_005246"
     CDS             complement(416..1618)
                     /locus_tag="ACHLCY_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0372261.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
BASE COUNT          501 a          433 c          348 g          495 t
ORIGIN      
        1 cgaaaaaata cttacaacac gggaaaaaaa aggaaacctg ataagcctgt ttgatggatt
       61 cactttattc cattgtgttg gaatgataac ctttcttatc cattatctca gaacacctga
      121 agaaaaagtt gaaaatatat ttatgttata tggtgcagat aaaaacaata aactacgcag
      181 aagactgatt tatgacgcac taggaataat tcagtctcag caggagtgaa gagttaaaca
      241 ggcaaattat tttttataaa aaggggattg ttaagtaatc ccctgttaat caaaatacgc
      301 tttcctgacg atctgataat tatcagctca atacatttga cataaaagcg gtttcttaat
      361 tactactgct acgatcacat atcaaatcat gatcgtacaa tatacatttt cactattatt
      421 tattcacaat ttgagacatc acataatccg cccatgcctg catcatcggt acacgctgct
      481 ccagtagatc agtacgatga tatgccgcct caaccttatt tttcagcgta tgagcgagcg
      541 ccctttccgc cagatcccgc gaatacccct gttcgctaca ccagtccctg aatgttgagc
      601 gaaaaccatg tgccgtggca actcgccccg gaatgtcact gacggctttc tttttacgca
      661 gaaaacttgt caacaccata tcggaaagga tctgctgctt tctgggtgaa gggaacacca
      721 gttcatcatg caggccacgt atattttcca gaatgtaaat agcctgccgg gataaaggaa
      781 cacgatgctg tatcctggct ttcattcttt ctgcaggtat agtccatacc cgcttatgaa
      841 aatcaatttc agcccagcgc attcccctgg cttcgcccga gcgagttgct gtaagtatca
      901 ccattaataa cagtgcgcgg gtaacattat aaggttcatc ggtatacaca ctggtcgcca
      961 caaaaagcgg taactgcctc cagggcattg cgggttggtg ttcatcacgt cctcttgtct
     1021 gctgaggaag caaatggtca accacatcaa caggatttgc tacacaaaaa ccgtgcgccc
     1081 atccccactg cataacaaca tgaatgcgct gtttaacccg gcttgccgtt tctgacaagg
     1141 ttaaccagac tggacgcagt gtttctgcca cgtccgcagc cgtaatcgaa tccagcgttt
     1201 ttgctcccag ttgaggaaac gcgtaattct caagcgtcga taaccactgc cttacatgct
     1261 ttggattttc ccatccagga gacagttctg catgtacacg cctggctgca tcggcaaatg
     1321 ttgggatagc gactttctca gattcagcct ttttaatctc cagaggatca tcacctgcag
     1381 caagttgctc tcgcattatc cgggcagtac gtgcagcttc agcaatactg acctctgggt
     1441 aagttcccaa tccagcatta cgtctttttt gtgtcaccgg acttacataa cgaaaaaccc
     1501 atttcccccg cccctttact gaagaaggat gaagggtcag tccggtaatt cccccatggg
     1561 gcaatggttt gtcatcaggt ttgatatgtc ttgctttcgt atccgtcaat actgccatac
     1621 gctaattccc gtcactctgg tatgccatcc agtatgccat tagtctctcg cttcactaag
     1681 aatacattag ataatatcgg acaacaaaaa gtgtaataca ttgtttataa tgaaattata
     1741 taacacccct ggattaagtc agatttattt caggcgg
//
LOCUS       DAZGTR010000326         1777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGTR010000326 DAZGTR010000000
VERSION     DAZGTR010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.329"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            132..>1777
                     /gene="tir"
                     /locus_tag="ACHLCY_005247"
     CDS             132..>1777
                     /gene="tir"
                     /locus_tag="ACHLCY_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFP0372262.1"
                     /translation="MPIGNLGNNVNSNNLIPPAPPLPSQTDGASRGGAGQLINSTGAL
                     GSRLLFSPLRNSIADSVDSRDIPGLPVHPSRLATATSEICLLGGFEVLHDKGPLDTLN
                     KQIGASAFRIEQQSDGSYAAIGEKNGVEVSVILNSQELQSLQAIDIEDKGRFVFTGGR
                     GGGGHSMVTPASDIAEARAKILAKLDPNNHGGSQARNVDTRSVGVGSASGMDDSVVSE
                     TRTSSTASSVRSDPKFWVSIGAIAAGLAGLAATGITQALALTPEPDDPTTTDPEQAAS
                     AAESATRDQLTQEAFKNPENQKVSIDEIGNSIPSGELKDDVVAKIEEQAKEAGEAARQ
                     QAVESNAQAQQRYDTQYARRQEELELSSGIGYSLSSALIVGGGIGAGVTTALHRRNQP
                     AEQTTTTTTHTVVQQQTGGNTPAQGGTDAIRAEDTSLNRRDSQRSTASTHWSDTSSAV
                     VNPYAEVGEARNSSPARQAEEHIYDEVAADPNYSVIQNFSGNNQVTGRLMGTPGQGIQ
                     STYAILTNNSAGLRLGMGGLTGSGGSAVNTANAAPTPGPGR"
BASE COUNT          505 a          361 c          470 g          441 t
ORIGIN      
        1 ggggggcatt attttgcttg catcaaaaat tagactgtga tttatttggt ttatgctcag
       61 ctgttttatc ggccgcatac cgttacgtcg ataaaggata atgtcaaatt tctaaataaa
      121 aggatatatg tatgcctatt ggtaaccttg gtaataatgt aaatagcaat aatttaattc
      181 cgcctgcgcc gccactacct tcacaaacag acggcgcgtc acggggagga gcgggtcaac
      241 taattaactc tacaggagca ttaggatctc gtttattgtt ttctcccctg agaaattcta
      301 tagctgattc tgtcgattcc agagatattc caggacttcc tgtacaccca tcgaggcttg
      361 ctactgctac atcagagata tgcttgcttg gaggatttga agttctccat gataagggac
      421 cacttgatac tctcaataag caaattggag cctctgcatt tcgtattgaa cagcagtcag
      481 atggttctta tgccgctatt ggagaaaaaa atggtgtaga ggttagcgtt atattaaatt
      541 ctcaagaatt gcaaagcttg caagctatcg atattgagga taaaggccga tttgttttta
      601 ccgggggacg tggtggtggt gggcattcca tggtcactcc tgcatcagat atcgcagaag
      661 ctcgtgcgaa aatactggcc aaattagacc caaacaatca tgggggaagt caagccagga
      721 acgttgatac gcgttctgtt ggtgttggaa gtgcttcggg aatggatgat agcgttgtta
      781 gcgaaactcg tacttcatca acagcttcca gcgttcgttc agatcctaaa ttctgggttt
      841 ctatcggcgc aattgctgct ggtttagcgg ggctggcggc tactggtatt acacaggcgt
      901 tggctttgac accggaaccg gatgatccta caaccaccga tcctgagcag gctgcaagtg
      961 ctgcagaaag tgcgacaaga gatcagttaa cgcaagaagc attcaagaat cctgagaacc
     1021 agaaagttag cattgatgag atcggaaatt ctattccgtc tggggaatta aaagatgatg
     1081 ttgttgctaa aatagaagaa caagctaaag aggcgggtga ggcggccaga cagcaggctg
     1141 ttgaaagcaa tgcacaggcg cagcagcgat atgatactca gtatgccaga cgtcaggagg
     1201 aattagagct ttcatcgggt attggttaca gcctcagcag tgcattgatt gttggtgggg
     1261 gaattggtgc tggtgtaacg actgcgcttc atagacgaaa tcagccggca gaacaaacaa
     1321 cgacaacaac aacacatacg gtagtgcagc agcagaccgg agggaatacc ccagcacaag
     1381 gtggcactga tgccataaga gcggaagaca catctctgaa tagacgtgat tcgcagagga
     1441 gtacggcatc gacacactgg tcagatactt ctagcgcagt ggttaatcca tatgctgaag
     1501 ttggggaggc tcggaatagt tcaccggctc gtcaggcaga agagcatatt tacgatgagg
     1561 tcgctgcaga tcctaattat agcgtcattc aaaatttctc agggaataac caagttaccg
     1621 gaaggttaat gggaactcca gggcaaggta tccaaagtac ttatgcgatt ctgacaaaca
     1681 acagcgctgg attgcgttta ggtatgggtg gattaacggg gagtggcggg agcgcagtaa
     1741 atactgcaaa tgccgcacca acgccgggac caggacg
//
LOCUS       DAZGTR010000327         1761 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGTR010000327 DAZGTR010000000
VERSION     DAZGTR010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.330"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..135)
                     /locus_tag="ACHLCY_005248"
     CDS             complement(<1..135)
                     /locus_tag="ACHLCY_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP0372263.1"
                     /translation="MEIANQLARSGIRFVPIPITADTELHLFGEILSRKLDELEKLVE
                     E"
     gene            complement(144..776)
                     /locus_tag="ACHLCY_005249"
     CDS             complement(144..776)
                     /locus_tag="ACHLCY_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFP0372264.1"
                     /translation="MTKKYTLIYADPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKRHHQLN"
     gene            complement(1003..1170)
                     /locus_tag="ACHLCY_005250"
     CDS             complement(1003..1170)
                     /locus_tag="ACHLCY_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372265.1"
                     /translation="MKFKFTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHAEVTNI
                     RIEELKEHQAA"
     gene            complement(1213..>1761)
                     /locus_tag="ACHLCY_005251"
     CDS             complement(1213..>1761)
                     /locus_tag="ACHLCY_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFP0372266.1"
                     /translation="LHSFLGVGRMFAHWVKERIAEYGFVESQDYILICQNGQTKGRGG
                     DRRSKDYHLTLDTAKELAMVERNEKGRQIRRYFIECEKKLRSMQPAQQFTDEEIILLC
                     YMQVQMEKAQDISKRLYPILKELNSSYASKLYDIAFETFYTVTKNRDALLREATRIDQ
                     TSAIFERARPMLKSLRARQFEF"
BASE COUNT          404 a          434 c          391 g          532 t
ORIGIN      
        1 ttcttctacc agcttttcca gttcatccag ctttcgggaa agaatttcac caaacagatg
       61 aagttctgta tctgctgtga tcgggatcgg aacaaagcgt attccacttc tggcaagttg
      121 atttgcgatt tccaggcact gccttaattc aactggtgat gccttttgtc aacgtcgttt
      181 tttcgctcat tggttttctc cagaaattta acaatacccg gaaccatctc aatggatggt
      241 gttccgcatt ggttacccca gacatcaaat ccatgcgagg tgtgacgggc gaacagttct
      301 atccggggaa catcgccaag aagttgcaca agtttttctc gcgccatatc cggcttgcgg
      361 gagtgatcaa ggcgcggtgc agtgaatgac tggattattc cggcgttaat gcgttcaggc
      421 agatttcctt tcactgcaaa aaggcagtct tcgctgttag cgcgagtggt gctacccata
      481 cccatgacca gcttgtcggt ctgtcttttc ccgcatttgt tccaggttaa tcctttcatc
      541 gtcaccagac gaaagcccca cgcttctaca accttcaatg cttcaagtgg ttgtgtaggc
      601 acccaccaca tagccaacag gcaattatcg gctgccagtt cccataccgg gaggcggcag
      661 atatccagaa gactcatgac cggatattta aaacttgcac cgcgttgacc atcggttgct
      721 ttgtctcgga atgtccaggg gggatctgcg taaatgagag tgtatttttt cgtcatagcg
      781 cgttttcaaa ctcatcaatg taaagtgcaa caagtgcaac gcatcgtctg aaatcggctg
      841 cgtcatgtgg agcaccgaag ctatgaccat agatattctt tttcagtcca catgcgattg
      901 aggcaagagt tgcgctactg atgccaacat agtcagtcga ttgccatttc aaaaccttca
      961 tagccaaatc tgacatttct tgtctccaat aaaaaaccgc catcaggcgg cttggtgttc
     1021 tttcagttct tcaattcgaa tattggttac ttctgcatgt gctatctgcg cccatatcat
     1081 ccagtggtca tagcagtcat tgatattctc cgcttcgata actctgttga atggttctcc
     1141 attccattca cctgtgaatt tgaatttcat taagtttcac ctgtaaaaaa aggagccgaa
     1201 gctcctttga tattaaaatt cgaattgtct cgcccgaagg ctttttaaca ttggtcttgc
     1261 ccgttcgaaa atggcgcttg tctggtcaat tcgtgttgcc tccctgagca atgcgtctct
     1321 gtttttcgtc accgtgtaga aagtctcaaa cgcgatgtca tacagcttgc tcgcgtatga
     1381 tgagttcagt tccttcaata tcggatacag gcgtttgctg atgtcctgtg ctttctccat
     1441 ctgtacctgc atgtagcaga ggaggatgat ttcctcgtct gtgaattgct gtgctggttg
     1501 catgctgcga agtttctttt cgcactcgat gaagtatcgg cgtatctggc ggcctttttc
     1561 gttacgctca accatcgcca gttctttggc tgtgtcgaga gttaggtggt agtccttgct
     1621 acggcgatca cctcctcgcc ctttggtttg cccattttgg caaatcaaaa tatagtcctg
     1681 gctttcaacg aatccatatt cagcaatgcg ttctttaacc cagtgcgcga acattctgcc
     1741 cacaccaaga aaagagtgta g
//
LOCUS       DAZGTR010000328         1756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGTR010000328 DAZGTR010000000
VERSION     DAZGTR010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.331"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..262
                     /locus_tag="ACHLCY_005252"
     CDS             <1..262
                     /locus_tag="ACHLCY_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFP0372267.1"
                     /translation="GLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKN
                     LAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
     gene            368..1261
                     /gene="galF"
                     /locus_tag="ACHLCY_005253"
     CDS             368..1261
                     /gene="galF"
                     /locus_tag="ACHLCY_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GalU regulator GalF"
                     /protein_id="HFP0372268.1"
                     /translation="MINLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEIVLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQAQLLGLGHSILCARPIVGDNPFVVVLPDVVLDTASADPLRYNLAAMVARFNETGRS
                     QVLAKRMDDDLSEYSVIQTKEPLDNEGKVSRIVEFIEKPDQPQTLDSDLMAVGRYVLS
                     ADIWAELEKTAPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKSIKKLLSE"
BASE COUNT          473 a          356 c          443 g          484 t
ORIGIN      
        1 gggacttgaa ccgaagaaca cggcggtgcg gagtccggag agtaacggaa tagcagagag
       61 cttcgtgaaa acgataaagc gtgactacat cagtatcatg cccaaaccag acgggttaac
      121 ggcagcaaag aaccttgcag aggcgttcga gcattataac gaatggcatc cgcatagtgc
      181 gctgggttat cgctcgccac gggaatatct gcggcagcgg gcttgtaatg ggttaagtga
      241 taacagatgt ctggaaatat aggggcaaat ccagtaacat acgcggcatc ggagtaatgt
      301 aattcatact ggctaaaaca agctaaaaag ctataattca gcaccaattg aataggtgaa
      361 tgacttaatg attaatttga aagcagttat tccggtagca ggtctgggga tgcacatgtt
      421 gcctgccacg aaggcaatcc ctaaagagat gctgccgatc gtagacaagc cgatgattca
      481 gtacattgtc gacgaaattg tagctgccgg tatcaaagaa atcgttctgg tgactcatgc
      541 gtctaagaac gcggttgaga accacttcga cacctcttac gaactcgaat cactccttga
      601 gcagcgtgtt aagcgccagc ttttagccga ggtacagtct atctgtcctc ctggcgtgac
      661 tattatgaac gttcgtcagg ctcagttact gggtctgggt cattccattc tttgtgctcg
      721 cccgattgtt ggggataacc cgtttgtcgt cgtattgcct gacgtcgttc tggatacagc
      781 gagtgcggac ccgttgcgtt ataacctggc tgccatggtt gcccgtttta atgaaaccgg
      841 gcgcagtcag gtgctggcta aacgcatgga tgatgatctg tccgaatact ctgttattca
      901 gaccaaagag cctttggaca acgaaggcaa agtcagccgt atcgttgaat tcattgaaaa
      961 acccgatcag ccacaaacgc ttgattccga tctgatggcc gtagggcgct acgtgctttc
     1021 tgctgatatc tgggctgagt tggaaaaaac tgctccaggt gcctggggac gtattcaact
     1081 gactgatgct attgcagagt tggctaaaaa acagtctgtt gatgccatgc tgatgaccgg
     1141 cgacagctac gactgcggta agaagatggg ctatatgcag gcattcgtta agtatgggct
     1201 gcgcaacctt aaagaagggg cgaagttccg taagagcatc aagaagctac tgagtgagta
     1261 gagatttaca cgtctttgtg acgataagcc agaaaaaata gcggcagtta acatccaggc
     1321 ttctatgctt taagcaatgg aatgttactg ccgtttttta tgaaaaatga ccaataataa
     1381 caagttaacc taccaagttt aatctgcttt ttgttggatt ttttcttgtt tctggtcgca
     1441 tttggtaaga caattagcgt gagttttaga gagttttgct ggatctcgcg gaactgctca
     1501 catctttggc atttagttag tgcactggta gctgttaagc caggggcggt agcttgccta
     1561 attaattttt aacgtataca tttattcttg ccgcttatag caaataaagt caatcggatt
     1621 aaacttcttt tccattaggt aaaagagtgt ttgtagtcgc tcagggaaat tggttttggt
     1681 agtagtactt ttcaaattat ccattttccg atttagatgg cagttgatgt tactatgctg
     1741 catacatatc aatgta
//
LOCUS       DAZGTR010000329         1739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGTR010000329 DAZGTR010000000
VERSION     DAZGTR010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.332"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(167..1354)
                     /locus_tag="ACHLCY_005254"
     CDS             complement(167..1354)
                     /locus_tag="ACHLCY_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFP0372269.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCSHIKKICFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQQWHPHI
                     HLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEGSSKR
                     DWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVYVITE
                     TVRKTAMQIRWRGMYQRLLKVDPLKCILCGGQMRFTGLKRGYRLTELVLMHEPLAQQR
                     VCG"
     gene            complement(1354..>1739)
                     /locus_tag="ACHLCY_005255"
     CDS             complement(1354..>1739)
                     /locus_tag="ACHLCY_005255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372270.1"
                     /translation="CLSGSYMSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAE
                     ALRFDNFDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILA
                     GISAGGMYCPECGTVHWPDGVPPPF"
BASE COUNT          411 a          516 c          412 g          400 t
ORIGIN      
        1 aaaaagatta aaaaaacacc caaaaacgga tgtttattca acactgcctg cgtgccatga
       61 gtgcaccgcc gtcaattaaa tggatcttat cgattgatac tgcatatcac tgatggtttt
      121 ttaccccatt cccctgaaaa tggacgcaac ttctccgatg cggctctcag ccgcacaccc
      181 gctgttgcgc cagtggctca tgcatcagga ccagctctgt cagacggtag ccccgcttca
      241 gccccgtaaa acgcatctga cctccgcaca ggatgcactt cagcgggtca accttcagta
      301 accgctgata catccctctc cacctgattt gcatcgccgt ctttctcacc gtctccgtta
      361 tgacgtacac aacatcttcc agtaaccggc gcttcaccgg actcaggaaa ccgtagtacc
      421 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccata aactcatcac
      481 cactcatcac caggtattct tcccgttttg tccggtgact gttgtaacgc agaccaattt
      541 catcctgacc agcatagtgc tccagacggc tcatcggcac cggcggtttt ttcaggtaag
      601 agccgaagta caccgccaca tgtgtggcgt tatccatcac ccgggatacg ttgacattcc
      661 agccccgccg gtaatgactg tccaggaagc gattccagtc ccgtttactg cttccttctg
      721 ctgccagcgc atccggtatc accaggtccg gatatttccg tgacagtaac cgcgttatcc
      781 ggtaacgcca catactcatc accttacggg cgtaaaaatg aaggtttttc caggtgtggt
      841 ctgatgtcac gccgccggtc gttgtcgaca ggtgaatgtg cggatgccac tgctggtcac
      901 gtccccatgt gtggatgacc gtgaatatcc ccggcaccac atctgcctgg cggcagattt
      961 cctgtatcac atccgcagca atgcggctca tctctgccag taaccaccgg ttgtggaaca
     1021 ccagggacca gtactggcag ggaagtgtga acacaatatg ctgccacgga cagtcgggaa
     1081 ccagactcag cagatactgt atccactgtg cgccagcctt cactccgcag tgcgggcagg
     1141 agcgactttt acaccggaag cagatctttt ttatgtggct gcagtccgga gatgaacagc
     1201 accactgtgt gtaccccatc agcgtggtcc cgcaggccat gatttttgtc acagactcaa
     1261 tgactaccgg acgcactgaa ccttccggtt gtttctccag ccagttaagc cagcggtttc
     1321 cctgctgaaa aatgtcggca aaacggggaa gcatcagaag ggcgggggga ctccgtccgg
     1381 ccagtgaacc gtgccacact ccgggcagta catgccgccg gcgctgatac cggcaagaat
     1441 ggtcgcaaac tcccgctccg tgcagcgggc tatttcagga tacccttcgt catcaacacg
     1501 tacaaaccag aagaccagct ttttgtttcc ggcatccaca aagaagggaa tattcaggtc
     1561 tgcgcagcac tcaacggcat cgtcaaaatt atcaaagcgc agagcttctg cgtcttcttc
     1621 gtcaaagaag tcatcgtcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgagg
     1681 ggcaggtttt cgtttacggg gtttagctga acgggacata taactacctg aaagacaat
//
LOCUS       DAZGTR010000330         1734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGTR010000330 DAZGTR010000000
VERSION     DAZGTR010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.333"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            2..523
                     /locus_tag="ACHLCY_005256"
     CDS             2..523
                     /locus_tag="ACHLCY_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0372271.1"
                     /translation="MRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRDGTGI
                     WTICRGAILVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIKVPLTEPQKAGIA
                     SFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQVSRR
                     DQESALACWGIDR"
     gene            794..1363
                     /locus_tag="ACHLCY_005257"
     CDS             794..1363
                     /locus_tag="ACHLCY_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP0372272.1"
                     /translation="MNMMAVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKQRNMVSMPLRKLAGWLQTINPNKVKPEIRDKVIRYQEEC
                     DDVLYEYWTKGFVVNPRKMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVS
                     KIRTLVNEANMLIDFVLADTGKGKITKAD"
     gene            1363..1509
                     /locus_tag="ACHLCY_005258"
     CDS             1363..1509
                     /locus_tag="ACHLCY_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372273.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            1517..>1734
                     /locus_tag="ACHLCY_005259"
     CDS             1517..>1734
                     /locus_tag="ACHLCY_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFP0372274.1"
                     /translation="MNRVLCVVIIVLAVGCGALWLATNHYRDNALTYKAQRDKKAREL
                     EQANATITDMQVRQRDVAALDAKYSRELA"
BASE COUNT          458 a          377 c          482 g          417 t
ORIGIN      
        1 aatcagatac ggcctgtcgg ctgccgttct ggcgctgatt ggtgcagggg cgtctgcgcc
       61 tgaaatcctc gaccagtttc tggatgaaaa ggaaggtaac cacaccacag cataccgtga
      121 tggtacgggt atctggacca tctgccgcgg tgccatcctg gtggatggta aacctgtcgt
      181 cccgggcatg aagttgtcga aggaaaaatg cgaccaggtt aacgccattg agcgtgataa
      241 ggcgctggca tgggtggaga aaaacatcaa agtgccgctg accgaacccc agaaagcggg
      301 gatcgcgtca ttctgtccgt acaacattgg tcccggtaag tgtttcccgt cgacgtttta
      361 cagacgaatt aatgcaggtg atcgaaaagg tgcctgcgaa gcgattcgct ggtggattaa
      421 ggacggtggc agggactgcc gtattcgctc aaataactgt tacggtcagg tatcccgtcg
      481 tgaccaggag agcgcgctgg cgtgctgggg aatcgacaga taagcagaat attttgctga
      541 aaaatgaggt ttgcttacat ggattgataa cacgaaatcc tgcaaattgg caaaatgtaa
      601 gtgaataaag tcaaaacagt tgtttaacac tcaggcaccg taatgatgcc tttgtcattt
      661 ctgcgcatct cacgcgcatc tcacaacaca gaacctttca ggatgaccct tgaggatacc
      721 ggtttggctg tcggtgcctt tctgtgggct ggattcctgt gagacaaggt tcatcactaa
      781 aaggaaataa ccgatgaata tgatggccgt gccgtttcac ggcaactctc tttatgtagt
      841 taaccataat ggcgaaccat acgttcccat gaaacctgtc gttgcgggga tggggctggc
      901 ctggcaatca cagttggcta agttaagaca gcgttttgcg tcaactataa cggaaatcgt
      961 tatggttgct gaggatggga aacaacgcaa tatggtgtcc atgccacttc gaaaacttgc
     1021 cggctggcta caaaccatta atcccaacaa agtaaaaccc gaaatccgcg ataaggtcat
     1081 ccggtatcag gaagagtgcg acgatgttct ttacgagtac tggacgaagg gttttgtcgt
     1141 taatccccgt aaaatgagcg tgatggaaga actcaaccag gcttgtgctg acatgaaacg
     1201 ggataaaaac attgccagtg tgtttgctac cgggctgaat gagtggaaac aggttaaagc
     1261 cgcgcatgta tcaaaaatcc gtacgctggt aaatgaagcg aatatgctga ttgattttgt
     1321 cctggctgat acaggcaaag ggaaaataac aaaggcggat tgatggggtg gctaatgata
     1381 tcagataaac tcataacgct ggtgaagagc ctctgtgtac ttgtcggcat ttcattttta
     1441 gtcatgctgg ttgccatttt cttttccacc gcctggcgag tcctgacgtt atcgggactg
     1501 gtggggtgaa agagagatga accgtgttct gtgtgtggtg attattgtcc tggcggttgg
     1561 ctgtggtgcg ctgtggctgg caacaaacca ttaccgtgac aacgcgctca cctacaaagc
     1621 gcagcgcgat aaaaaagcca gagagctgga acaggcgaat gccaccatta ctgacatgca
     1681 ggtgcgccag cgtgatgttg ctgcgctcga tgcaaaatac tcgagggagt tagc
//
LOCUS       DAZGTR010000331         1722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGTR010000331 DAZGTR010000000
VERSION     DAZGTR010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.334"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(124..252)
                     /locus_tag="ACHLCY_005260"
                     /pseudo
     CDS             complement(124..252)
                     /locus_tag="ACHLCY_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(283..1445)
                     /locus_tag="ACHLCY_005261"
     CDS             complement(join(283..1184,1184..1445))
                     /locus_tag="ACHLCY_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFP0372275.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWWRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          411 a          448 c          394 g          469 t
ORIGIN      
        1 tctataaaaa ggttgtgatt atttacataa gtaatagaat ttagataatt tttattgtga
       61 cgataaaata taccattaat gattgattta gagacactgg ctttctgaat cgccacggat
      121 aatctagaca cttccgagcc gttgataata ctggttttca tattctgtcg gtgacatctg
      181 ttcgctagta ccatgccgac gcttactgtt ataaaacatt tcgatgtaat caaaaatatc
      241 actgcgggct tctgatccta cccacgtaat atggacacag gcctaagcga ggttcttgtt
      301 ttcaaattgt tccggactga ggccgccaca ccaactgtgc cgccgccacc gattgtaatc
      361 acattcgata taattaaata ccgttgcccg cattatttcc cggctgataa agtgttctcc
      421 atggatacat tccactttca gcgaatgaaa gaagctttcc acgcaggcat tatcgtagca
      481 gcaacctttt gcgctcatac ttccacgcag attatgccgc ttcagttgcg cctgataatc
      541 tgctgaacag tactggcctc cacggtccgt gtgaacgata acgttccggg gcctcttacg
      601 ccaccacagc gccatctgca gggcatcgca ggccagttgc gccgtcatgc gtggcgacat
      661 tgaccagcca ataacggcac gtgaccacag gtcaatgacc actgccagat acagccagcc
      721 ttcatctgta cgtaagtacg tgatgtctcc tgcccacttc tggttcgggc cactggcgta
      781 aaaatcctgc tccaacagat tttctgacac aggcaggccg tgtgcgcggt agctgaccgg
      841 gctgaacttc cgggaggcct ttgccctcag tccctgacgg cgcaggcttg ccgccacggt
      901 ttttacgtta aaggggtaac cctgagcacg cagttcatcc gtcaggcgtg gggcaccgta
      961 acgctgtttt gaccgggtaa aagccgcgag gacaacgctg tcgcagtgtt ggcggaactg
     1021 ctgacgcgtg cttatccttg tccgccgctg acaccacgta taccagccgc tgcgggccac
     1081 ccggagcacg cggcacattg ctttgatgct gaactcagcc tgatgttttt caataaagac
     1141 atacttcatt tcaggcgctt cgcgaagtat gtcgcggcct tttggaggat agccagctct
     1201 tcatcccgtt ctgccagctg gcgtttgaga cgtgcaatct cggtagacat ctccagttca
     1261 cgttcagaag acgtctgctg attttgctgt ttactgcgcc agttgtagag ctgtgattca
     1321 tacaggctga gttcacgggc tgcggcagta acaccgatgc gttcagcaag cttcagggct
     1381 tcactgcgaa attcaggcga atgctgttta cggggttttt tactggttga tactgttttt
     1441 gtcatgtgag tcacctctga ctgagagttt actcacttag ccgcgtgtcc actattgctg
     1501 ggtaagatca tatctccccg gcagccttca ggaaaatatt atcagataac tgcgtaaaaa
     1561 aagaacaaat ggttgtgtcc gctattgcca gtacacctga gtttacagcc tttcagatta
     1621 tcacaatcaa tagcgaacag acttttgagc tatgtgcgct tttaacaaaa cgcccctttc
     1681 tccactttca ccgtataata atacattatt aattacaatc cc
//
LOCUS       DAZGTR010000332         1667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGTR010000332 DAZGTR010000000
VERSION     DAZGTR010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.335"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..491)
                     /locus_tag="ACHLCY_005262"
     CDS             complement(<1..491)
                     /locus_tag="ACHLCY_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein EspH"
                     /protein_id="HFP0372276.1"
                     /translation="MSSSLSGITFATSLTSNQASWSKLTRTLHISNDDTVDELQLKAR
                     MQQRHHRVFPDIDDYSVINFRGKEYAVRFIKENDNYVYKVHKITQESGCFSKIFSFLY
                     GGVTKALESKLNERHITPLSSTWFPRTALEGFLTERGLSSLLRRVQSTEGVNPPENTT
                     FNS"
     gene            complement(522..1439)
                     /gene="escQ"
                     /locus_tag="ACHLCY_005263"
     CDS             complement(522..1439)
                     /gene="escQ"
                     /locus_tag="ACHLCY_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP0372277.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSEGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDTIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGKNRLNVIILETTVQSLQYLINDNWQFVPHS
                     NTLFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWLVINNPLATVKLSDNNL
                     SITDVQAADPLCTISSETVMNRIYCAIGTINVDIHMLRNVQKDDIIDSTGYHLLGGCQ
                     LVRNNITIAYGSIIKINEDFYFTISVVCD"
     gene            complement(1402..>1667)
                     /gene="escP"
                     /locus_tag="ACHLCY_005264"
     CDS             complement(1402..>1667)
                     /gene="escP"
                     /locus_tag="ACHLCY_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE needle length
                     regulator EscP"
                     /protein_id="HFP0372278.1"
                     /translation="WKRLFYSSGRRRRYFEEGEQSFSILCGRLRGIVLTIKCSNGIIY
                     LSITVSPNNRNHVFLYHKKDYVFDKLKEIFPDETIEFTIEYEN"
BASE COUNT          521 a          360 c          276 g          510 t
ORIGIN      
        1 aaagagttga atgtagtatt ctctggaggg ttcacacctt ctgtagactg tacgcgacgt
       61 aacaaagaag aaagccctct ttcagttaaa aagccttcca gtgcagtgcg aggaaaccag
      121 gttgaagaaa gtggcgtaat gtgtctctca tttaatttac tttccagggc cttcgtaacg
      181 cctccataca aaaagctaaa tatctttgag aaacatccac tttcctgagt tatcttatgt
      241 actttgtata cataattatc attctcttta atgaagcgga cagcatactc ttttccccga
      301 aaattgataa ctgaatagtc atcaatgtct ggaaaaaccc ggtggtgacg ctgctgcatg
      361 cgtgctttaa gttgcagttc atctactgta tcatcattcg atatatgaag agttcgggtc
      421 aacttactcc atgacgcttg attgcttgtc aaagaagtcg cgaatgttat acctgataat
      481 gatgacgaca tcatacacct ccctatataa cgcactcctg attaatcaca tactacgcta
      541 atagtaaaat aaaaatcttc gttgattttt ataatacttc cgtatgctat tgtgatatta
      601 ttccgtacta actggcaccc acccaaaaga tgatatcccg ttgaatcgat aatgtcatct
      661 ttttggacat tacgcaacat atgtatatca acatttattg tgccaatcgc acaataaatg
      721 cgattcatta cagtctcact gctgatagta cataacggat ctgcggcctg aacatccgta
      781 atagataagt tattatcgct caactttacg gttgccaaag ggttattaat aacaagccaa
      841 caccttctct cttgactatc atcaacatga tagagtctca gactatcacc aactctcaac
      901 tctgtaaccg gataatcagt ccacccaggt acgatatagc catcaaaaaa aagcgtatta
      961 gaatgtggaa caaattgcca attatcatta atcagatatt gtaaactttg taccgttgtt
     1021 tcgagtataa ttacattaag tctgtttttt ccattattat tttctattac cggataaaca
     1081 tctttattta gcatcgtaaa ttgaacatca tcaactgaca catcaccttc cggtaaggca
     1141 gtctgcatta cccataagat catgagtttt atcgtatctt ccggtatcag ccggctttcg
     1201 ctatacggga ataactcctg gcaccactga atgaactgtt cttctgatat ccaaaaacgc
     1261 ccctcatagc ccgagtatgt caacgtgaaa cgaataccat gaccgctgac ttcacgaaaa
     1321 tatgcatggc acgcatgacc ttcggacgta atatagaggc gaccggttcc gatgactggc
     1381 aacaatggca aataaaaatc gttaattttc atattcaatt gtgaactcaa tggtttcatc
     1441 aggaaagatt tcttttaact tatcgaaaac atagtctttt ttatgatata aaaaaacatg
     1501 atttctatta ttggggctta ctgtaataga cagataaatg attccgttac tacatttaat
     1561 tgttaaaaca attcctcgta atcttccaca aagaatacta aacgactgct ccccttcttc
     1621 aaagtagcgt cgtcgtcggc ccgaggagta aaacaatctc ttccata
//
LOCUS       DAZGTR010000333         1658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGTR010000333 DAZGTR010000000
VERSION     DAZGTR010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.336"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1339
                     /locus_tag="ACHLCY_005265"
     CDS             <1..1339
                     /locus_tag="ACHLCY_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP0372279.1"
                     /translation="ETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQ
                     QDIVPGILGLGCARNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLF
                     RNMVPVTLLKEHYRCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNF
                     SNLRELESLEGHYWDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIV
                     FSTVLDKKRSSQHSRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIR
                     YIKYYADDGEIFESPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDI
                     LSQDSYRSMMFHSQIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVI
                     EVDGGYHLTSVQAERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
BASE COUNT          468 a          301 c          391 g          498 t
ORIGIN      
        1 agagacatac agggatgaat ttgaccgttt tataaagcgt tttccgatta ttggtagcag
       61 tacgcattcg attattaact ctatcggcaa gggggccctt cttgattatg ttattattga
      121 tgaagcctcg cagcaggata ttgttccggg cattctggga ctgggatgtg cccgtaatgt
      181 aatcgttgta ggggatcgta agcaacttcc acatgttcct gtattactcc cgaacagccc
      241 atctccaccg gcagaatact ataattgtga gaaatatagt ctacttgatt cggtttgtat
      301 gttgttcagg aacatggtgc cagtaaccct tcttaaagag cattatcgtt gtcatcctaa
      361 aatcattcaa ttttgtaata agcagtttta tgataatgct ctgataccgt taacggttga
      421 ttccggtgaa gcctcattat ctctggtcat cacggcaaaa ggaaaccata cccgtaattt
      481 ttcaaatctc agggagcttg aatctctgga aggtcattac tgggatgaag aaagtagcag
      541 gggatacatt gccccatata atgcacaggt taatctggct gaaaaagtgc tgcctgctga
      601 ttttgtgaaa tcgacggtgc ataaatttca gggacgggag tgcgatgaaa ttgtgttttc
      661 cactgtgctg gataagaagc gcagcagcca gcacagtcgg aacatcgcgt ttgttgataa
      721 tcctgaactg gtgaatgtcg ctgtttcaag ggcccgaaat aaatttacgc tggtgactgg
      781 taatgatgta tttgaacgcc acgctggaca tattgcggcg ctgattcgtt acatcaaata
      841 ctacgctgat gacggagaga ttttcgagag cccggtcata tcagcatttg atttgctgta
      901 cagtgagtat gacaaatcac tggaacgact gaatagtaga ttgaattcaa atgattcaca
      961 ctttaagtca gaacagattg tggcctgcct gttgcgggat atcctgtcgc aggatagtta
     1021 tcggagtatg atgtttcatt cacaaatagc gctgaatcag ttggtgttgc tggagcgggg
     1081 ggattttaca caccgtgaac agttattcat gcgtaaccgg gccagttgtg actttgttgt
     1141 ttactataag gtcgggaaga ctccattagg ggttattgaa gtggatggag gttatcatct
     1201 gaccagtgtt caggctgaac gtgatgaact taaaaacagt atactgaaga aatgcggcct
     1261 gccattgcta agattacgta ctatagacag tgatatagaa gggaagttgg gagcgtttct
     1321 gtcaggcctg acaggctaaa atctgaataa aaaatattgc agcgggaggt ctcaaacctg
     1381 atttggtcat gtgccacaca caattgtacg aaccccatgg acacaggctt ctttgttttg
     1441 gttacatcaa cgtacgtatt cctgatacat ataaaaataa gtgctattgc tcaaagctaa
     1501 cagggaatgt ttctgttgaa taattcctta gtgaataaac tttatcagtg ggtaattcta
     1561 ttacagtgaa tacttcctgc aaaatgatat ttgaattgtt caccatctgc atcatatttt
     1621 cttatttaca agttgaaagg atagtatatg ttgaaaat
//
LOCUS       DAZGTR010000334         1642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGTR010000334 DAZGTR010000000
VERSION     DAZGTR010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.337"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..336)
                     /locus_tag="ACHLCY_005266"
                     /pseudo
     CDS             complement(1..336)
                     /locus_tag="ACHLCY_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(652..1296)
                     /locus_tag="ACHLCY_005267"
     CDS             complement(652..1296)
                     /locus_tag="ACHLCY_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372280.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1317..1586)
                     /locus_tag="ACHLCY_005268"
     CDS             complement(1317..1586)
                     /locus_tag="ACHLCY_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0372281.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
BASE COUNT          419 a          424 c          321 g          478 t
ORIGIN      
        1 tgattctgga aaaggacttt gtggattgtt gctattgcca gtgagattga atgaagtcgt
       61 tttaactcgc tttgaatacg tcttgccccc agatttcgct gagaacgtaa ctccagaatc
      121 agagcgactt cgcaagtgcc ggttttcgtt gatggtgaac gcttggggcg acggctgtgg
      181 ctttccagac cagcgatgcc ctgagccaga tatctgcgcc accattttac gcaatgtggg
      241 ccgagaaatg ccacagcgac ggcatacaaa tcccgcatca ccggacgttt catagagttt
      301 tacccactgt agccgctgct gaatttccct gttcataatt tggcattagt tcagctcaat
      361 cccgacttaa aaagtcccta caggatgttc cggacctgag gcacagttca tatccttatc
      421 cataaaaaat ttagctatgt ctatagcatt aatcttgaca agacgacgtt agggcctggc
      481 gatgaatcat ttcccctgcc agatatcctt tggggtgcac cagaactacc gttttatatc
      541 tatataactg aaacgtccct cgccatcctt catattttga tgtagtaaca ccattcactt
      601 tcatctaccc cccgttttat taacatatag taaaaatcac ctcgccgcat tttattcttt
      661 tgaagtaaaa tcatttacca gttcacttaa agctctcttt tgttcatcat catacagaga
      721 ttgaatccgg aatttaatct cttctgcaac cagtcttgca tcgggctcat aaaatatttt
      781 tgttattcgc tgtttctgat gttcaggctg gtcttccata tttcgtaaaa ccagatgaaa
      841 taatatatca taaagtgcgc aatgccatga ataaccatca ttggttaaaa taagctccca
      901 aacccgctcc tgctcggcaa taagctgcag aacaaaggac tcaacctgtt ctgtttgttc
      961 gggggcaaca cggtatagcg gagcaatcac agcaaaagat agttcattta accaggattc
     1021 cgcttccttc tcgctcctga acggtggaca gttttgtatt cttcgttcgt attcacgggt
     1081 aatctgctcc agaatatcct ccggcggtgt cagccactta ttcctgagca atacactggt
     1141 tgatggatga cagctggcta tttcagcaat aatttcacgt aatgtctccg ggctgaactt
     1201 cttcagctga ttgtagtgct cccgggtaaa accatgttca tcgcataaaa gttgccatgt
     1261 cattgatata ctgtttgcca ttccttgctt catcattaat acctgttacc tgaatatcaa
     1321 cagaccggtg taccttttct gaatacctcc ccggacggag tggcaatccc cattcgcctc
     1381 agcgtggcat ctgcagcttt cgccacctcg ctgatatcca gcgtttcagg tagcggcggg
     1441 gacgatatct gcgccagaca ataattccgg cgcaacaaca accactcccg ttgtgcttct
     1501 gtacattcaa catttcccat aacaaaaagc cagcggtcag aagctctgcg ccagcaacca
     1561 gcagcctcca gtttctgcgc aaccatgtct tccacaactt ccggggtact ctttttcctg
     1621 ctcaaaacat taactcactg aa
//
LOCUS       DAZGTR010000335         1635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGTR010000335 DAZGTR010000000
VERSION     DAZGTR010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.338"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..963
                     /locus_tag="ACHLCY_005269"
     CDS             <1..963
                     /locus_tag="ACHLCY_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372282.1"
                     /translation="CKIFLSIVSIMETINSGGNLIPNSTSNKGYVCIDMQCSSTSEPT
                     ASTSSNRSIKLAASTNVYPITRNDSELTLNDFLDNSSSTSSLDYINELGSQLTLNDFL
                     DNIKTNEVDRTCTDVVINIPQEIQTNTQENDLLLSDKNNSICIEIDERITKILTCKQK
                     YQLDSIIHEIIPKENESAETVLHLMRVLNDQYHQVYNQSGCFYKAYMAIHNKIEQILP
                     YAFRAGGGISIHLLIQALFFNGDYNKSPSQSQQPSLYTSPSPTINTEAFLSNVLSLDI
                     TQVRILGDLLSATLFHAPTIFYQYPKLIDEVKYCISNKKITVRL"
     gene            1002..>1635
                     /locus_tag="ACHLCY_005270"
     CDS             1002..>1635
                     /locus_tag="ACHLCY_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372283.1"
                     /translation="MSPLLMLNGAVKTGSIVRTIGRGVSYVDIPLALAILGDSWYKAY
                     KHGSSDNPNSAQRFISQEAAFKTTQRVLTQGLSLMSSLSGAIMRSLEKGTPPQMMSLF
                     IVNILNLLFHQNPYEGASASANALKRSTYSHNPDILNTQAIALCVDLQHTKNITMPLF
                     KTKDRISYAFNGQRTSPEDQKQILKEVINSCTQGERAILNTSQSETCKHKI"
BASE COUNT          604 a          321 c          255 g          455 t
ORIGIN      
        1 tgcaaaatat ttttatcaat agttagtatt atggaaacca ttaattcagg aggaaacttg
       61 attccaaatt caacttcaaa taaaggttat gtatgcattg acatgcagtg ttcgtcaacc
      121 tctgaaccaa cagcttctac ctccagcaac cggagtatta aattagccgc ttccacaaat
      181 gtatatccga taacaagaaa cgactccgag ctcactctga acgattttct tgataatagc
      241 tcttctacgt catcattgga ctacattaat gaattgggtt ctcaactgac gttaaatgat
      301 tttcttgaca acataaagac aaatgaggtg gatagaacat gtacggatgt ggtaattaat
      361 atcccacaag agatacaaac aaatacacag gaaaatgatt tgttattatc cgataaaaat
      421 aattcaatat gcattgaaat cgatgaaaga attacaaaaa tcctaacatg caagcaaaaa
      481 tatcaactgg acagcatcat tcatgaaatt ataccaaaag agaatgagag tgcagaaact
      541 gttctccatc ttatgagagt tctgaacgat caatatcatc aggtatataa tcagtcagga
      601 tgtttttata aagcctatat ggccatacac aataaaatcg aacagatact tccatatgcg
      661 ttcagagccg gaggcggaat cagcattcac ttgctcatac aggcattatt ttttaatggc
      721 gactataaca aatcaccttc acagtctcaa caaccatctt tatatacatc accttctcca
      781 acaataaata cagaagcatt cttaagtaat gtattatcac tagatataac ccaggtacgc
      841 atacttggtg atttactatc agcaacttta tttcatgcac caacaatatt ctatcaatat
      901 cctaaactaa tagatgaagt taagtattgt ataagtaata aaaaaataac agttcggtta
      961 tagcacgatt tactctatgt ttaacaagta cattactaac catgtcacca ctgttaatgc
     1021 ttaatggagc agttaaaaca ggtagcatag taagaactat aggtagggga gtgagttatg
     1081 ttgatatacc attggcctta gctatattag gtgactcgtg gtataaagct tataaacatg
     1141 gttcttctga taacccaaat tctgctcaga gatttatatc gcaagaagcg gcctttaaaa
     1201 ccacccagcg ggtattaaca caaggattaa gtctgatgtc ctctttatcg ggagcaatca
     1261 tgcgctctct tgagaaaggc acaccaccac aaatgatgtc tttattcatc gtaaacatac
     1321 taaatctatt atttcatcaa aatccatatg aaggggcatc agcaagtgct aatgctttga
     1381 aaagatcaac ttactcccat aatccggaca tactaaatac tcaggcaata gctctttgtg
     1441 ttgacctcca gcatacaaaa aatataacca tgccactttt caaaacaaaa gacaggatat
     1501 cttacgcatt caatggacaa agaacatccc cagaagacca aaaacaaata ctgaaagaag
     1561 ttataaactc ctgtacccaa ggagaaagag ccattttaaa tacatcacaa tcagaaacat
     1621 gcaaacataa aatcg
//
LOCUS       DAZGTR010000336         1633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGTR010000336 DAZGTR010000000
VERSION     DAZGTR010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.339"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            174..1565
                     /locus_tag="ACHLCY_005271"
     CDS             174..1565
                     /locus_tag="ACHLCY_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0372284.1"
                     /translation="MTENTTPTADFSPSKRFFVSMLTRDIDLNDAILDLLDNCVDGAL
                     RTIKDTKKTSKPYEGFYAKLTINKDVFIIEDNCGGIPKSFREYAFKMGRPHQKEEENE
                     GTVGVYGIGMKRAIFKMGRDCSIQSNNPDGAFTVDITPDWIDGDGWKIPMHESDYDNK
                     NPTGTTIEIKKLHSNVAQKFNESTYLTDLFLQIKHSLSFIIQKGFKIELNGVVVEHNP
                     INIITDHSKIEPYIYKAKIDDVDIDLVVGFYKNLEDDNDDVLEKRSSDDAGWTVICND
                     RVVLYCDKTHLTGWGFANVPRFHTQFIAISGVVRFTSKNPEKLPITTTKRGVDLSSTL
                     YNDVRNKMIEGMMHFIRFTNQWKGEHLEEGKKLLKSAQSHEAQSLFEITPQSTPEDKK
                     NNWSNPNRNKNEWRYTPKLPTPVKKSSTVRIIFTREKEDVKILSKYFFGHEDASASDV
                     GMQCFDTVIEEVK"
     gene            1565..>1633
                     /locus_tag="ACHLCY_005272"
     CDS             1565..>1633
                     /locus_tag="ACHLCY_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372285.1"
                     /translation="MSGQYIPYHLRHNKSIDREIFLE"
BASE COUNT          588 a          275 c          298 g          472 t
ORIGIN      
        1 tcctctcacg ggattcagtc ctgaattctc ataaagctaa tcttttttta aaaacatact
       61 gttagatcac atgcactttc tttcttaaca tcgttgactt cgcatgcgaa gcgtttaaaa
      121 tcaacaattt gcacttaagg tatttatttt cccaaacaca tagagagtaa attatgactg
      181 agaacacgac tccaacagca gactttagtc ctagtaaaag attttttgta agtatgctaa
      241 ctcgagatat cgacttaaac gatgccatac ttgatctgct tgataactgc gtcgatgggg
      301 ctttacgtac aataaaagac acaaaaaaaa cttcaaaacc atatgaagga ttttatgcga
      361 aacttacgat taataaagat gtctttatta tagaagacaa ttgcggaggt atccctaaaa
      421 gcttccgaga atatgctttt aaaatgggac gaccccatca aaaagaagaa gaaaatgaag
      481 gcaccgttgg cgtctatggt attggtatga aaagagccat ttttaaaatg ggtagagatt
      541 gctcaataca atcgaataat cccgatggcg cattcactgt agatataaca cctgattgga
      601 tcgatggtga tggttggaaa ataccgatgc acgagagtga ctatgataac aaaaatccaa
      661 caggcacaac tatagagata aaaaaactcc attctaatgt ggcacaaaaa tttaatgaga
      721 gcacatatct tactgatctc tttttacaga taaaacactc attgtcattt attattcaaa
      781 aaggatttaa aatagaatta aatggagttg ttgttgaaca taatccgatt aacatcataa
      841 ctgatcattc taaaatagaa ccatacatat ataaagctaa aatagacgat gttgatatcg
      901 atttagttgt tggcttctat aaaaatttag aagatgacaa tgatgatgtt ttagaaaaaa
      961 ggagttctga tgatgctgga tggactgtca tttgcaatga ccgcgttgtt ctatattgtg
     1021 ataagacaca tctcacaggt tggggctttg ctaatgtacc aaggtttcat acccaattta
     1081 tagcaatttc tggggttgtt cgctttacca gtaaaaaccc agaaaaatta ccaataacaa
     1141 caacgaaacg aggtgtcgat cttagttcta cattgtacaa tgatgtacga aataaaatga
     1201 ttgaagggat gatgcatttt attcgattca ccaatcagtg gaaaggtgag catcttgaag
     1261 aagggaaaaa attacttaag tcagctcagt cacatgaagc tcaatcttta tttgaaatta
     1321 ccccacaatc aactccagaa gataaaaaaa ataactggtc caaccctaat agaaacaaaa
     1381 atgagtggcg atatacacct aagctgccta ccccagtaaa aaaaagctct actgtacgaa
     1441 taatcttcac cagagagaaa gaagatgtta aaattttatc taaatatttc tttggacatg
     1501 aagatgctag cgcttctgat gtggggatgc aatgctttga tacggtgatt gaagaggtga
     1561 agtgatgtct ggacaataca ttccctatca cttaaggcat aataagtcta ttgatagaga
     1621 gatttttctc gaa
//
LOCUS       DAZGTR010000337         1613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGTR010000337 DAZGTR010000000
VERSION     DAZGTR010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.340"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            21..1214
                     /locus_tag="ACHLCY_005273"
     CDS             21..1214
                     /locus_tag="ACHLCY_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0372286.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
BASE COUNT          341 a          414 c          469 g          389 t
ORIGIN      
        1 catggagttg aaacattaaa atgtcacctg ggatcgaaga tacgccccaa aaaccactgt
       61 cctgctggcc acttgcgttc agtgccgggc ttctcggtat cgggcagaac ggtctgctgg
      121 ttgtgctccc tgttctggtc atacagacaa atctgagtct gtctgtatgg gctgccctgc
      181 tgatgcttgg ctcaatgctg tttctgccat cgtccccgtg gtggggaaag caaatttccc
      241 gtactggcag taaacctgtg gtgttgtggg ctctgggagg atatggcata agctttaccc
      301 tgctagggct gggaagcgtg ctgatggcta ccagcgccat aacaacagcg gtgggactgg
      361 gaatattaat catcgcccgg atcgcctacg ggctgaccgt gtcggcaatg gtgccagcct
      421 gtcaggtctg ggcattgcag agagcaggag aagggaatcg catggccgct ctggcaacca
      481 tcagctccgg cctgagttgc ggcaggctat tcgggccgct gtgcgcggca gcaatgctgg
      541 ccattcatcc tctggcgcca ctggggctgc tgatggcagc cccagtgctg gcgctgctga
      601 tgctgctgcg gttgcccggc acaccaccac agcccacacc ggagcgcaag agcgtcagtc
      661 tgaagcggga ttgtctgcct tatctgcttt gcgcaatatt actggctgcg gcggtgagca
      721 tgatgcagct tggactttcg cctgccctta ctcgccagtt cgccacggat accaccgcca
      781 ttagccaaca ggtggcctgg ctgttgggtc tgtccgcagt agctgcgctt atcgcgcagt
      841 tcggggtact ccgtccgcag cgcctgactc cggtggccct gctcctgagt gccggagtgt
      901 tgatgagtgg tggtctggct atcatgctct ccgaacagct atggttgttt tacccgggct
      961 gtgcagtgct gtcatttgga gcagctctgg caacacccgc ttatcaactt ctactgaatg
     1021 ataagctggc tgatggcgca ggcgcgggct ggctcgctac cagtcacaca cttggctatg
     1081 gactgtgcgc tttgctggtg ccgctggtat cgaaaacagg tgtcgcaatc gcgctgatta
     1141 tggcggcatt atttgcaact atattattta ccattgtgtc tgtatttatc tggcattacc
     1201 gtgctatcaa ataaagatat gatcatcacc ttaccggagt aactcagagg atatgagaga
     1261 gaacctgcgt ctgatgaaag catgtcaggc agtaacactg gcaaaaatgc accgcatggt
     1321 acagggtatg ttcggtataa aaccggccgg tgtcacttaa gttgcattga agtcatgctg
     1381 gttgtgagtg caacatcaca tggctctgtc cggatgcctg cagacaatga gaacagtgcg
     1441 attactcatt gggaatgaga tgaacgcatc cccaacatta gatctggggc accgtgataa
     1501 ataaagggga aaatcagaga ggtgtcgagc ctcgcagatt tccggcatca tagtgcccaa
     1561 tacgggatgc ataaaactct ctcagagtgg cgttgacacc tgaaagagct gga
//
LOCUS       DAZGTR010000338         1610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGTR010000338 DAZGTR010000000
VERSION     DAZGTR010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.341"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..152
                     /gene="wbdI"
                     /locus_tag="ACHLCY_005274"
     CDS             <1..152
                     /gene="wbdI"
                     /locus_tag="ACHLCY_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-sugar hydrolase WbdI"
                     /protein_id="HFP0372287.1"
                     /translation="HYIVLCYTLKVLKSELNLPDDQHREYLWLTKHQINAKQDVHNYS
                     KNYFL"
     gene            186..>1610
                     /locus_tag="ACHLCY_005275"
     CDS             186..>1610
                     /locus_tag="ACHLCY_005275"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFP0372288.1"
                     /translation="MSQCLYPVIIAGGTGSRLWPLSRVLYPKQFLNLVGDSTMLQTTI
                     TRLDGIECENPIVICNEDHRFIVAEQLRQIGKLTKNIILEPKGRNTAPAIALAAFIAQ
                     KNNPNDDPLLLVLAADHSINNEKAFRESIIKAMPYATSGKLVTFGIIPDTANTGYGYI
                     KRSSSADPNKEFPAYNVAEFVEKPDVKTAQEYISSGNYYWNSGMFLFRASKYLDELRK
                     FRPDIYHSCECATATANIDMDFVRINEAEFINCPEESIDYAVMEKTKDAVVLPIDIGW
                     NDVGSWSSLWDISQKDCHGNVCHGDVLNHDGENSFIYSESSLVATVGVSNLVIVQTKD
                     AVLVADRDKVQNVKNIVDDLKKRKRAEYYMHRAVFRPWGKFDAIDQGDRYRVKKIIVK
                     PGEGLDLRMHHHRAEHWIVVSGTAKVSLGSEVKLLVSNESIYIPQGAKYSLENPGVIP
                     LHLIEVSSGDYLESDDIVRFTDRYN"
BASE COUNT          533 a          259 c          333 g          485 t
ORIGIN      
        1 cacattatat agtgctttgt tacacactga aagttcttaa aagtgaattg aatctcccag
       61 atgatcaaca tcgtgaatac ctttggctaa ctaaacacca aataaatgct aaacaagatg
      121 ttcataacta ttcaaaaaat tattttttgt aatttttatt aaaaattaat atgcgagaga
      181 attgtatgtc tcaatgtctt taccctgtaa ttattgccgg aggaaccgga agccgtctat
      241 ggccgttgtc tcgagtctta taccctaaac aatttttaaa tttagttggg gattctacaa
      301 tgttgcaaac aacaattacg cgtttggatg gcatcgaatg cgaaaatcca attgttatct
      361 gcaatgaaga tcaccgattt attgtagcag agcaattacg acagattggt aagctaacca
      421 agaatattat acttgagccg aaaggccgta atactgcacc tgccatagct ttagctgctt
      481 ttatcgctca gaagaataat cctaatgacg accctttatt attagtactt gcggcagacc
      541 actctataaa taatgaaaaa gcatttcgag agtcaataat aaaagctatg ccgtatgcaa
      601 cttctgggaa gttagtaaca tttggaatta ttccggacac ggcaaatact ggttatggat
      661 atattaagag aagttcttca gctgatccta ataaagaatt cccagcatat aatgttgcgg
      721 agtttgtaga aaaaccagat gttaaaacag cacaggaata tatttcgagt gggaattatt
      781 actggaatag cggaatgttt ttatttcgcg ccagtaaata tcttgatgaa ctacggaaat
      841 ttagaccaga tatttatcat agctgtgaat gtgcaaccgc tacagcaaat atagatatgg
      901 actttgtccg aattaacgag gctgagttta ttaattgtcc tgaagagtct atcgattatg
      961 ctgtgatgga aaaaacaaaa gacgctgtag ttcttccgat agatattggc tggaatgacg
     1021 tgggttcttg gtcatcactt tgggatataa gccaaaagga ttgccatggt aatgtgtgcc
     1081 atggggatgt gctcaatcat gatggagaaa atagttttat ttactctgag tcaagtctgg
     1141 ttgcgacagt cggagtaagt aatttagtaa ttgtccaaac caaggatgct gtactggttg
     1201 cggaccgtga taaagtccaa aatgttaaaa acatagttga cgatctaaaa aagagaaaac
     1261 gtgctgaata ctacatgcat cgtgcagttt ttcgcccttg gggtaaattc gatgcaatag
     1321 accaaggcga tagatataga gtaaaaaaaa taatagttaa accaggagaa gggttagatt
     1381 taaggatgca tcatcatagg gcagagcatt ggattgttgt atccggtact gctaaagttt
     1441 cactaggtag tgaagttaaa ctattagttt ctaatgagtc tatatatatc cctcagggag
     1501 caaaatatag tcttgagaat ccaggcgtaa tacctttgca tctaattgaa gtaagttctg
     1561 gtgattacct tgaatcagat gatatagtgc gttttactga cagatataac
//
LOCUS       DAZGTR010000339         1603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGTR010000339 DAZGTR010000000
VERSION     DAZGTR010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.342"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            41..310
                     /locus_tag="ACHLCY_005276"
     CDS             41..310
                     /locus_tag="ACHLCY_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372289.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCMVRPLQILRADGRWENIGGMK"
     gene            423..998
                     /locus_tag="ACHLCY_005277"
     CDS             423..998
                     /locus_tag="ACHLCY_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP0372290.1"
                     /translation="MPLTSDIRSHSFNLGVEVVRARIVANGRGDITVGGETVSIVYDS
                     TNGRFSSSGGNGGLLSELLLLGFNSGPRALGERMLSMLSDSGEAQSQESIQNKISQCK
                     FSVCPERLQCPLEAIQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLVGEGLPHP
                     LTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            1071..>1603
                     /locus_tag="ACHLCY_005278"
     CDS             1071..>1603
                     /locus_tag="ACHLCY_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFP0372291.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTSNKILMEKINSCLFRPDSNHFSCPESFLTCPITLDTPETGVFMRNSRGAEI
                     CSLYDKDALVQLVETGGA"
BASE COUNT          457 a          272 c          414 g          460 t
ORIGIN      
        1 gatgaagtgg gcagtgatac aggcagaaca ggagaatgac atgaatattt tgagaaagct
       61 tatggagcgt ctgtgtggtt gcggaaagca tgatgactgt gaacacgggc agtcgcttac
      121 agtacaactg cgactggggc cggcagacat cctggagtca gatgagaatg gcattattcc
      181 ggagcaggac agggtaatca cacaggtggt gatactggat gcggataaaa agcagataca
      241 gtgcatggta agaccgctgc aaatcctacg tgctgacggg aggtgggaaa atattggcgg
      301 gatgaaatag ccgacgggtt cacaaaaacc ggagtcctgc tctggttttt gttgtcacgt
      361 atgggggatg tttgttacag ctatttaagt ctggagttca aattaaaata gggagctttg
      421 ttatgccatt aacctcagat attagatcac attcatttaa tcttggggtg gaggttgttc
      481 gtgcccgaat tgtagccaat gggcgcggag atattacagt cggtggtgaa actgtcagta
      541 ttgtgtatga ttctactaat gggcgctttt catccagtgg cggtaatggc ggattgcttt
      601 ctgagttatt gcttttggga tttaatagtg gtcctcgagc ccttggtgag agaatgctaa
      661 gtatgctttc ggactcaggt gaagcacaat cgcaagagag tattcagaac aaaatatctc
      721 aatgtaagtt ttctgtttgt ccagagagac ttcagtgccc gcttgaggct attcaatgtc
      781 caattacact ggagcagcct gaaaaaggta tttttgtgaa gaattcagat ggttcagatg
      841 tatgtacttt atttgatgcc gctgcatttt ctcgtttggt tggtgaaggc ttaccccacc
      901 cactgacccg ggaaccaata acggcatcaa taattgtaaa acatgaagaa tgcatttatg
      961 acgataccag aggaaacttc gttataaagg gtaattgaaa tgaacattac cctttatttt
     1021 atttaatgaa acatcctgca aactgatatg aattactgaa tgaggttttt atgcctgtta
     1081 ccaccttaag tatcccaagt atatctcaat tatctcctgc aggagtacag tctttgcagg
     1141 atgctgccag acttgaaagt ggaataagaa tatccattgg tagtggccaa tattctgttc
     1201 actatgtcca gctactggat ggattttcag ttgaaccggt gagaggaggc ttactggata
     1261 ggctattggg gcgtgagcat cgaatggaga gaagggctgt ggctctggaa aggcaattaa
     1321 atggaggtgt cgatttttta agtagtgtta ataactattt tcagagtgtc atggcagaac
     1381 acagagaaaa taaaacaagt aataaaatat taatggaaaa aataaattct tgtttattta
     1441 gacctgattc taatcacttt tcttgcccgg agtcattttt gacatgcccg ataacgctgg
     1501 acacacctga gactggggtg ttcatgagaa actcacgagg tgctgagata tgctctctat
     1561 atgataagga cgcgttagtg caacttgttg aaactggtgg agc
//
LOCUS       DAZGTR010000340         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGTR010000340 DAZGTR010000000
VERSION     DAZGTR010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.343"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            231..>1594
                     /locus_tag="ACHLCY_005279"
     CDS             231..>1594
                     /locus_tag="ACHLCY_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP0372292.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVA"
BASE COUNT          452 a          298 c          405 g          439 t
ORIGIN      
        1 aacagttttc ttattaatca acaataactc atagttcatt tttaaataaa ataagaacta
       61 tgcatttttt aatctatcaa ttcgaaagta ttagcaaatt agccaaattg acaccttccc
      121 acctccaact aacactcaat acagacctct gacaatttaa taagtttcat ttttaatgtc
      181 tgacgactca tctctttcgt ggctgtattg tttgtatatg gagcgtttat atgaatagaa
      241 tctatcgcgt gatatggaat tgcactctac aggtatttca ggcctgctcg gaattaactc
      301 gcagggcagg taaaacatcg acggttaatt tgcgtaaatc ctctggactg acaacgaaat
      361 tcagtagatt gacgctgggt gttttgctgg cactaagcgg ttcagcgtct ggtgcaagtc
      421 tggaagttga taatgatcag attaccaata ttgatactga tgttgcttat gatgcctacc
      481 tggttggctg gtatggcact ggagtgctta atattttggc tggcggtaat gcctccttaa
      541 ccactattac taccagcgtc attggcgcta atgaggactc agagggcacc gttaatgttt
      601 tgggtggcac ctggcgattg tatgatagcg gaaataatgc aaggccttta aatgtgggtc
      661 aatccggaac ggggacgctg aatattaaac agaagggtca cgtcgatgga ggctatttaa
      721 gattaggttc ttcgacagga ggcgtcggga cggtcaatgt tgagggagag gactctgttc
      781 tgacgaccga attattcgaa atagggagtt acggtacggg ctcattaaat attacggata
      841 agggttacgt cacgagttca atcgtcgcca ttttaggcta tcaggcgggc agtaatggcc
      901 aggttgtcgt tgaaaagggt ggcgagtggc taataaaaaa taacgattcc tcaattgaat
      961 ttcaaattgg taatcaagga actggggagg cgactattcg cgagggtgga ctgattacgg
     1021 ctgaaaatac gattatcggt ggcaatgcca ccggtgtcgg aaccctgaat gtgcaggatc
     1081 aagactctgt catcacggta cgcagactct ataatggata tttcggtaat ggcacactca
     1141 atatttccaa taatggacta attaataaca aagaatattc attagtgggc gttcaggacg
     1201 gttcccacgg tgtcgtcaac gtgaccgata aagggcattg gaatttcctc ggaacgggcg
     1261 aagctttccg ctatatctat atcggtgatg ctggcgacgg tgaacttaat gtctcgcgtg
     1321 aaggtaaagt agattcggga attatcactg cggggatgaa agaaacaggc acaggcaaca
     1381 ttactgttaa ggataagaac tccgttatca ctaatctcgg aactaatctt ggttatgacg
     1441 gccacggcga aatgaatatc agtaatgaag ggcttgttgt cagcaacgga ggaagctcac
     1501 tcggttatgg agaaaccggc gtcgggaatg tcagcatcac cacggggggg atgtgggagg
     1561 tcaataagaa tgtctatacc accattggtg ttgc
//
LOCUS       DAZGTR010000341         1574 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGTR010000341 DAZGTR010000000
VERSION     DAZGTR010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.344"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..338)
                     /locus_tag="ACHLCY_005280"
                     /pseudo
     CDS             complement(1..338)
                     /locus_tag="ACHLCY_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559742.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(347..1114)
                     /gene="sctT"
                     /locus_tag="ACHLCY_005281"
     CDS             complement(347..1114)
                     /gene="sctT"
                     /locus_tag="ACHLCY_005281"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFP0372293.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1116..1376)
                     /locus_tag="ACHLCY_005282"
     CDS             complement(1116..1376)
                     /locus_tag="ACHLCY_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0372294.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1386..>1574)
                     /locus_tag="ACHLCY_005283"
     CDS             complement(1386..>1574)
                     /locus_tag="ACHLCY_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0372295.1"
                     /translation="IYLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGW
                     TMLSKGLILQYFDLSINP"
BASE COUNT          544 a          325 c          277 g          428 t
ORIGIN      
        1 aacttaaatt taacagcttc agttgctaat tgcattttac tttgaaacca ggagacaagt
       61 attgttgtga acatgcaaac cagcaaaaaa ggaatgttcg ttttcaacca accaaccccc
      121 atagctttaa aatagtccca aatatctggt ttagcgttat gatcaaggat gtattcaaga
      181 atcgacataa tgtgatgcac atcaaaaaca tatccgagat ataaagtacc cacaagcatt
      241 attacagaga tggttaagtc tctactttat agaaattgcc cttttttaga agcatcctgg
      301 agtttctttt gtgttggctt ttcagtttta ttggccatga aacactctat ttctcaatgt
      361 atagttgcaa ttgttctggg aaaaaggata acggcattac tcgttcagcc agtattgggg
      421 caaaataaat aattaatatt aaaaatgcca gcgcactttt gacagtcagt gaaatggcaa
      481 aagcatttag ttgtgaagca taacgtgcca gcaatcccag gacagcctca ccgcccagca
      541 taacggcaat cacggggctt gcgtaaacga tagtatgagt cattatgtta ttaatatatg
      601 agaataaagg ctctaattta ggaaaattaa aattgcctga aggccataaa ttataacttt
      661 gccaaagagt ttccagaata aagttcattc cacctttagt aagataaacc gcagcagaga
      721 aaaggttaaa taatcttgct aattccgagg tatcgacacc tgtagccgga tccaaagtac
      781 tacttaatgt tgcaccgcgt tggttatcga taaagctacc tatagcaagg aaaatccaaa
      841 acggggatgc taataagcat gcaataaaca gccctatgat aatttctctg gcagcgaaca
      901 aagcaatatc gatttcattt aagttcaata attcatagtg gtaatgctga actaatgcac
      961 atgataccac tattattatt gggtgacgaa ctactgttgg tatattccca ctggcgaaaa
     1021 atggtaacaa atagaaagtt ggtgccaatc ggcaaaaacc gagcgctgta gcagccagca
     1081 gactatgcaa ctgatagagt atcgcctccc ccatgctatc ctctcgcgaa tgctaaattc
     1141 agcatttcga taccaaatga atataatttt tcaccatacc acccgctcat taaaaaaaag
     1201 caaatactta cgcacaataa tttcacgcca aatggcaacg tctgttcttg caactgcgtt
     1261 accgtttgaa aaagccctac caataaacca acaaatgtcg caactgcaat aggtcctgca
     1321 gacataacta aaatcagata cagcgcccta ttccccgcaa aaacaatatc gtccatattt
     1381 tttcctcagg gattaatgga taggtcaaaa tattgtaaaa tgagcccttt tgataacatt
     1441 gtccaaccgt ccatcgcgac aaacaaaatt aattttatcg gtgtagatat ggttaccgga
     1501 ctcatcatca tcatacctaa cgttaacaga acgctggata taaccagatc cactacgaca
     1561 aagggtaaat agat
//
LOCUS       DAZGTR010000342         1537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGTR010000342 DAZGTR010000000
VERSION     DAZGTR010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.345"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..666
                     /locus_tag="ACHLCY_005284"
                     /pseudo
     CDS             1..666
                     /locus_tag="ACHLCY_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            680..1432
                     /locus_tag="ACHLCY_005285"
     CDS             680..1432
                     /locus_tag="ACHLCY_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0372296.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
BASE COUNT          391 a          324 c          437 g          385 t
ORIGIN      
        1 cacacgccgg gcgcaatccg tctgtgctgg cactgcgata accagctgcg cgatcagttc
       61 acggaacggc tggaatcaat ggcaacggat aactgtaccc gctgggtgtt gtctgttgtg
      121 cgtcgggatc tcggttttga tgacagtcac gttgtgacaa tgccggaact gtgctggtgg
      181 ctgattcgta atgacctggc ggatgcctta ccggaaagtg cagcccgtaa ggcactgaga
      241 ttaccgaagc ctgttgtgcc gtctgtcacc cgggaaagtg accttgtgcc ttcggttcct
      301 gccaccagca tcatccagga taaagcgaaa aaggtgctgg cgctgaaagt ggatccggag
      361 tcgccggagt cttttatgtt acgcccaaaa cgtcgccgct gggttaatga aaagtacacg
      421 cgctgggtta agacatagcc gtgtgcatgt tgtggaaagc ccgctgatga tccccaccac
      481 ctgataggcc acggtcagtg tggaatgggt acaaaagcgc atgacctttt tgtgttgcct
      541 ttgtgcagaa agcatcacga cgagctgcat gcggataccg tggcatttga agagaagtat
      601 ggctctcagc tggagctgat atttcgtttt atcgatcgtg cgctggcaat tggcgtactg
      661 gcgtaagtgg agaacgagca tgaaccttga agccttaccg aaatattact ccccgaaatc
      721 tccaaaactg agcgatgacg caccggcgac aggctctggt ggtttaacaa ttacggatgt
      781 gatggctgcg caggggatgg tgcagtcaaa agcaccgctt gggtttgctt tattcctggc
      841 aaaagttggt gttcaggatc ctcagtttgc gattgaaggt ctgctcaatt acgcgatggc
      901 actggataac ccgacattga ataaattgag tgaagaaacc cggctacaga tcattcctta
      961 ccttgtgaat tttgcctttg ctgattattc caggtctgcg gcaagtaagg ctcgctgtga
     1021 gcattgtgct ggtactggat ttcataatgt attgcgcgag gtggtgaaac actccagaag
     1081 cggggaatct gttattaagg aagagtgggt gaaggaacta tgtcagcatt gtcatggtaa
     1141 gggagaagtc agcacagcat gcagagggtg caagggtaaa ggtattgtcc tggatgaaaa
     1201 aagaacccgg cttcatggca cgcctgttta taaggtttgt gggcgttgca atggaaaccg
     1261 gtttagccgt ttaccaacca cactggcgcg gcttcatgtc cagaagctgg taccagacct
     1321 gacggattat cagtggtaca aaggatatgc agatgtcatt gataaactgg ttacaaagtg
     1381 ctggcaggaa gaagcatatg cagagataca attgagaaag gtgacaagat aaatggtttt
     1441 cgccgaagat gacgacatga tgcttgcatt tttcaaaaaa tatggataag attttcccaa
     1501 cgatgggctt tgtatgtcta ccgttgataa gatttaa
//
LOCUS       DAZGTR010000343         1526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGTR010000343 DAZGTR010000000
VERSION     DAZGTR010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.346"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..249
                     /locus_tag="ACHLCY_005286"
     CDS             <1..249
                     /locus_tag="ACHLCY_005286"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0372297.1"
                     /translation="MLVPEDMSVGWFSKALESVDEVRIITDGRINFIEPSTGLEKKGN
                     SKGSMLLIWRPFISPRRMFTTVSKAALMAIGRGVRRAA"
     gene            246..428
                     /locus_tag="ACHLCY_005287"
     CDS             246..428
                     /locus_tag="ACHLCY_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0372298.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            425..595
                     /locus_tag="ACHLCY_005288"
     CDS             425..595
                     /locus_tag="ACHLCY_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP0372299.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCSDCCAELMSD
                     PNSSMYEEEDDE"
     gene            588..1310
                     /locus_tag="ACHLCY_005289"
     CDS             588..1310
                     /locus_tag="ACHLCY_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory/antirepressor protein"
                     /protein_id="HFP0372300.1"
                     /translation="MSELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEVSERINNLGFNVQYEHYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMERGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            1310..>1526
                     /locus_tag="ACHLCY_005290"
     CDS             1310..>1526
                     /locus_tag="ACHLCY_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0372301.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCSPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLAL"
BASE COUNT          469 a          333 c          409 g          315 t
ORIGIN      
        1 atgcttgtgc cagaggatat gtcagtcgga tggttcagca aggctctgga gagtgtcgac
       61 gaagttcgca ttatcactga tggacggatt aattttatcg aaccatcgac agggctggag
      121 aagaagggaa acagtaaagg ctccatgctg ctgatttggc gaccgttcat cagtcctcga
      181 cggatgttta ctaccgtatc caaagcggca ttgatggcga tcgggcgggg cgtcaggagg
      241 gcggcatgag acgacagcga cgaagtatca ccgacatcat ctgcgaaaac tgcaaatacc
      301 ttccaacgaa gcgatccaga aataaacgca agccaatccc aaaagaatct gacgtaaaaa
      361 ccttcaacta cacggctcac ctgtgggata tccggtggct aagacatcgt gcgaggaata
      421 caaggtgatt gacccaaatc gaagttacga acaagaaagc gtcgagcgag ctttaacgtg
      481 cgctaactgc ggtcagaagc tgcatgtgct ggaagttcac gtgtgctccg attgctgcgc
      541 agaactgatg agcgatccga atagctcaat gtacgaggaa gaagacgatg agtgagttaa
      601 taaatggcaa tgccatcaaa atgacaagca ttgaaatcgc tgagttggtg ggtaagcgtc
      661 atgacaatgt gaaacgtacc atcgaaacgc tggctaaaaa tggtgttatc cggcttcctc
      721 aaattgaggt ttccgaaaga atcaataact tagggttcaa tgttcagtac gagcattacg
      781 tcttcgaagg cgaacaagga aagcgagaca gtattgtcgt tgtagcccag ctgtcgccgg
      841 aattcaccgc tcgccttgtt gaccgctggc gagagcttga agaagctgcg gttaatatcc
      901 ccaaaacgct accagaagcg ttgcgccttg ctgctgatct tgctgagcag aaaatgcaac
      961 tggaaaacca gctcgcaatt gccgcaccta aagttgagtt tgccgatcgc gttggcgagg
     1021 ccagcggaat tttgattgga aactttgcaa aggttgttgg tattggtcca aacaaactgt
     1081 ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg ttcccggcgc aatgtgccaa
     1141 tgcaggaata tatggagcgc ggctatttca cagtgaaaga aacagcggtc aatacaaatc
     1201 acggaataca gatatcgttc accacaaaaa tcaccgggcg tggtcaacag tggctgacca
     1261 gaaagctgct cgataacgga atgctgaaag ttacagggga ggctgcttaa tggctaaacc
     1321 agcgcgaagg aaatgcaaaa tctgtaagga atggtttcac ccggcattct caaatcagtg
     1381 gtggtgcagc ccggaacacg gaactcaatt agcactcgaa cgacgaagca aagaacgcga
     1441 aaaagcggaa aaggcagcag agaagaaacg acgacgagag gagcagaaac agaaagataa
     1501 actgaagatt cgaaaactcg ccttaa
//
LOCUS       DAZGTR010000344         1525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGTR010000344 DAZGTR010000000
VERSION     DAZGTR010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.347"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            99..902
                     /locus_tag="ACHLCY_005291"
     CDS             99..902
                     /locus_tag="ACHLCY_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP0372302.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPRNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWA"
     gene            961..>1525
                     /locus_tag="ACHLCY_005292"
     CDS             961..>1525
                     /locus_tag="ACHLCY_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFP0372303.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASIDVLRKAVFEGANIIITHEPTFYSYADLEGDVLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAIEP
                     DSAINITKRFAKNLKIYNLPGIQFYGDR"
BASE COUNT          434 a          252 c          327 g          512 t
ORIGIN      
        1 atatagatat tagtcatttc taagtgctcc catatataat gtggtagggg agtgcttaga
       61 tgtaatattt cttttgttta ttgaggttaa tttaaattat gaacgatatt gagaactatg
      121 ggaatcgata tcatgtgttg caatcctttc cgatgccaca attggcaagt agtcgcgaaa
      181 taagaatatt actacctgcg tcctattatg aatctgatgt cagttaccct gtattgtata
      241 tgcacgatgg gcaaaattta tttgataaat ctgtatcttt tggtccacat gtatgggata
      301 tacctgaggc ggtagatgct tttttcccaa attcccaata cgacggtgtt atcattgtgg
      361 ggattgataa tgcatcgtct catggtaaat tcagccgtat ggatgagtat tctccatggc
      421 cgcgaaacac atcttttccc ttacctggct gggaccctga tgtggattat agtggaggaa
      481 aaggtgcgtt gtatgttgat tttattgtga atacgctcaa gaactatatt gacagcaatt
      541 ttcgaactct cccagatcgt aataatacag cgatagcagg tagttctatg ggtgcataca
      601 tcagtttgtt tgccgcaatt cttcgtctag acgttttttc aaaagttggc gttttttcgc
      661 cggcactatg gttcaatgac agcgcaatgc tgaattttat tcaggaaaac aatatctttg
      721 cagatcttac tgtatatctc gatgtcggta cgctggaaac cagtggtatg cgcgaagact
      781 ttcctgaagt ctatatttca ggcgcggaaa aattatgcgt gagtctccgt aaacaacgca
      841 atgtaactat agattatcat ctatggggag gtgataccca ctctgagtct gcatgggcat
      901 aacgttttcc agaaatgttg aaattatttt attgctgatg aaaccggtga aggatgttga
      961 atgcatcata ttaaaacagc cgcagatctt aattgttttt taaattcaat ggttgccgtt
     1021 tcgcaaccaa ctgttgataa aattattttt ggtgctgagg cgacacttat taataaaatt
     1081 gggacctgct ggattgcctc aattgatgtt ttaagaaagg ctgtttttga aggtgccaat
     1141 atcattatta ctcatgagcc tacattttat tcttatgctg atctggaggg tgacgttctt
     1201 gagttttcat gggccagaaa aataatggat tatacgcgcg gtgaactcag ttatttaaaa
     1261 ataattgagc aaaaaaagga gtttttgcat aaaaataatt tggtaatcat tcgttgccat
     1321 gatgtcatgg atcgagagcc tacgttcgga atgtctaagg ctttggctca gcagttagaa
     1381 ctggatgtta caaatattgt tgcttctgat gatatgtacc atgtatatgc tatagaacct
     1441 gacagtgcca ttaatattac caaacgtttt gccaaaaatt tgaaaattta taatcttcct
     1501 gggatccaat tttatggaga tagag
//
LOCUS       DAZGTR010000345         1506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGTR010000345 DAZGTR010000000
VERSION     DAZGTR010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.348"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(177..1052)
                     /locus_tag="ACHLCY_005293"
     CDS             complement(177..1052)
                     /locus_tag="ACHLCY_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFP0372304.1"
                     /translation="MFSIRTLLPISASVSVPTKQSQSIPITLAGRTIEKAQEKEGLLV
                     FLGMKSVNDYTLNILGQNVSRVTTGKKPYDLLFLNDATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISITATEMKTILPDDLTLGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PSDIVDRIVQHNLHEIDDIRLTSCNSANIIKNKDFSPDEIEKSANMNNGWLARALFGQ
                     KRSLAEHVYAEFERRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEHTVRRS
                     DYRATLGRTQPIDIE"
     gene            complement(1193..1462)
                     /locus_tag="ACHLCY_005294"
     CDS             complement(1193..1462)
                     /locus_tag="ACHLCY_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372305.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
BASE COUNT          385 a          346 c          290 g          485 t
ORIGIN      
        1 tcgccagaac cgtattgata tttactgagc gctcagatca actttccatg gcaacagatc
       61 gcgtacccgg tttgccggcc agtcctggat atgctcccgc cgacaatatt aatggccagg
      121 aaatgtttaa aacaatgatt ctgtttccta accatcaagt gtatatgctg gctcaattac
      181 tcaatatcta tgggttgagt tctacccaaa gtggctctgt agtcgcttct tcttacagta
      241 tgttcaggtg ttgcaggcac agttgtggag cgtagatgcg ttgttggttt accatgctct
      301 ggtacataaa aaacgccagt gccatggtaa cctgatatag aaacgttaat tccgcgacgt
      361 tcaaactcgg catagacgtg ttctgctaaa gacctctttt gaccaaataa tgccctggcc
      421 aaccagccgt tattcatatt tgcggatttt tctatttcat caggagagaa gtctttgttt
      481 tttattatgt tggctgagtt acaggatgtt aatctgatat catctatttc atgaagatta
      541 tgctgaacaa tgcggtcgac aatatctgac ggtgataaaa attcatctcc gcacttaaga
      601 agaggtagac cagcagaacc atgcccagac agataaattt tgttgtattt tcctagtgtt
      661 aaatcatctg gcaggatggt tttcatctct gtcgctgtaa tacttatagc tgcagcagca
      721 acaacatcgc tattacttga ttgtagatgg cttttatttg ctccaggata tgtaaactcc
      781 attttccttt tatcaaaatc ttgttttgta gcatcattca ggaataataa atcatacggt
      841 tttttccccg ttgtgactct tgaaacattt tggccaagaa tattaagagt atagtcatta
      901 acggatttca ttcctaaaaa aacaagtaat ccttctttct cttgcgcttt ttcgattgtt
      961 ctccctgcta aagttattgg gatggattga gattgttttg tcggaactga tacgctggcg
     1021 ctaataggta gtagggttct tatgctaaac atacaacctc tttcttaatt ttcgaacctg
     1081 tttaggattc tgtgtaaatt caaaataaac ctgtctgaac attcctaaca aacatccacc
     1141 ggacatgaca acaaaaaccg gagccggact ccggtttttg tgaagctgtc ggctatttca
     1201 ttccgccaat attttcccac ctcccgtcag cacgcagaat ttgcagcggt cttaccacgc
     1261 actgtatctg ctttttatcc gcatccagta tcaccacctg cgtgattacc ctgtcctgct
     1321 ccggaataat accattctca tcggactcca ggatgtctgc cggccccagt cgcagttgtg
     1381 ctgtaagcga ctgcacgtgt tcacggccat catgctttcc gcaaccacac agacgctgca
     1441 taagtttttt tagtatattc atgtcattct cctgttctgc ctgtatcact gcccacttca
     1501 ttcagc
//
LOCUS       DAZGTR010000346         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGTR010000346 DAZGTR010000000
VERSION     DAZGTR010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.349"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..628)
                     /locus_tag="ACHLCY_005295"
     CDS             complement(<1..628)
                     /locus_tag="ACHLCY_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0372306.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQK"
     gene            636..1061
                     /gene="tnpA"
                     /locus_tag="ACHLCY_005296"
                     /pseudo
     CDS             636..1061
                     /gene="tnpA"
                     /locus_tag="ACHLCY_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          414 a          356 c          312 g          409 t
ORIGIN      
        1 tcttctggtt tttctgaaaa ctgtttacag gctcaaagac tgtgccatct gacagcgtgg
       61 cgagtttagc cacgccagca tccagcccga ccattgatgc tgaagggtga accggagttg
      121 atacttcact ttctgtctga atactgatgt accacttacc gcaggactgg ctgacagtga
      181 catttttcac aacacccgtg acctgacggc tgttccggta gcgcatccag cccagtttcg
      241 gcagaaaaat acggctgttt tcctgatcga gcttaacgcc ctgcgggtag cggaatacat
      301 cattctgtcc ccgctttttg aatcggggaa aagcagcccg cttctggaag aagtttttgt
      361 aagcccgctc aaggtctttc agtgactgtt gcaatggctg tgacggggca tctttaagcc
      421 attgcgtttc agtggcattt ttccactcaa ccagccagga agccattttc ccgtaaggga
      481 tgtatttatt accgacctca tgattctcat tctgacgtgc cagagcacga ttgaaaacga
      541 aacgacatgc gcctgcgaag cgcctcattt cacgctcctg ttgaccaccg ggtcttaact
      601 ggaatttaaa tgcctgtaat cgtttcacag gccaaatgat actttggtct gtgaaaaaag
      661 aaaccgatat ccggcgtgga aggcattgtg ttttcctgat gcatgttcac ctggtctttg
      721 tcaccagata ccgacgtcag atttttgacc atgacgcgac agaaaaacta cgcacttact
      781 tttcaaatgt atgtgctgat tttgaagctg aactggttga aatggatggc gaaccagatc
      841 acgtccattt gttaattaac tatcctccca aactggcgat atccagtctg gtaaacagcc
      901 tcaaaggcgt atcgggtagg tgactgcgac gagatcgacc agatattgca gtcaggtatt
      961 actacaaagg cgttttgtgg agtgctggct attttgccag tagctgcgga ggtgcgccaa
     1021 tatccgtcat ccgccaatac attgaacaac agcaaacacc tggtcaggtg gaaaaccgcg
     1081 ccttatatcc ctgccctgaa ggacggggtt ttacggcgca ccggataagc ctggagatta
     1141 tctacaccag catggacatc attgtactat gccccgtaca tgccccatca catcctcaac
     1201 atcaaagtat tagagccttg caaaccatta tgcgtctcag tttgtctcat ctatcttacg
     1261 caaaaaaaat agaactaata tcaagcggcg ctatattgcg tattaaataa aaccacctca
     1321 attacattaa taaatccaaa caaacatcat gcaaataatt cccatggtta tttctaggct
     1381 atttaaatta aataaataaa ttatgagtca atcataaata acaaccttta atataatttg
     1441 actaccaaat ttatattcgt tcacaaaata gtcatgcatt gtgtgcaata g
//
LOCUS       DAZGTR010000347         1491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGTR010000347 DAZGTR010000000
VERSION     DAZGTR010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.350"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..841
                     /gene="nleB"
                     /locus_tag="ACHLCY_005297"
     CDS             <1..841
                     /gene="nleB"
                     /locus_tag="ACHLCY_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP0372307.1"
                     /translation="EARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDG
                     NFTYEQKKEFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQESYPASERDN
                     YLLGLLREELKNIPEGKDSLIESYAEKREHTWFDFFRNLAILKAGSLFTETGKTGCHN
                     ISPCSGCIYLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALL
                     AGLDIMKSKVDAHPYYDGLGKGIKRHFNYSSLHNYNAFCDFIEFKHENIIPNTSMYTS
                     SSW"
     gene            890..>1491
                     /gene="nleE"
                     /locus_tag="ACHLCY_005298"
     CDS             890..>1491
                     /gene="nleE"
                     /locus_tag="ACHLCY_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFP0372308.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVVYRNDSPLGEQR
                     ANRFIELYNIKRDIMQELNYELPELKAVKSEMIIAREMGE"
BASE COUNT          533 a          217 c          302 g          439 t
ORIGIN      
        1 tgaagcaagg ccagagcgat acgaaaaagg agaagtacca atattgaata ccaaagaaca
       61 tccgtatttg agcaatatta taaatgctgc aaaaatagaa aatgagcgta taatcggtgt
      121 gctggtagat ggaaatttta cttatgaaca aaaaaaggaa tttctcagtc ttgaaaatga
      181 atatcaaaat ataaaaataa tctaccgagc agatgtggat ttcagcatgt atgataaaaa
      241 actatctgat atttaccttg aaaatatcca taaacaagaa tcataccctg ccagtgagag
      301 ggataattat ctgttaggct tattaagaga agagttaaaa aatatcccag aaggtaagga
      361 ctctttgatt gagtcatatg cagaaaaaag agaacatact tggtttgatt ttttcaggaa
      421 tttggccata ttgaaggctg gaagtttgtt tacagagacg ggaaaaactg gatgccataa
      481 catatcgccc tgtagcggat gtatatatct tgatgccgac atgattatta ccgataaatt
      541 aggagtcctg tatgctcctg atggtatcgc tgtgcatgta gattgtaatg atgagataaa
      601 aagtcttgaa aatggtgcga tagttgtcaa tcgtagtaat catccagcat tacttgcagg
      661 cctcgatatt atgaagagta aagttgacgc tcatccatat tatgatggtc taggaaaggg
      721 tatcaagcgg cattttaact attcatcgtt acacaattat aatgcttttt gtgattttat
      781 tgaatttaag catgaaaata ttataccgaa taccagtatg tataccagca gttcatggta
      841 agtcttccgg ttgtggttga aaaatatcgt tctttaggta tagattcaga tgattaatcc
      901 tgttactaat actcagggcg tgtcccctat aaatactaaa tatgctgaac atgtggtgaa
      961 aaatatttac ccggaaatta aacatgatta ctttaatgaa tcacccaata tatatgataa
     1021 gaagtatata tccggtataa ccagaggagt agctgaacta aaacaggaag aatttgttaa
     1081 cgagaaagcc agacggtttt cttatatgaa gactatgtat tctgtatgtc cagaagcgtt
     1141 tgaacctatt tccagaaatg aagccagtac accggaagga agctggctaa cagttatatc
     1201 cggaaaacgc ccaatggggc agttttctgt agatagttta tacaatcctg atttacatgc
     1261 attatgtgag cttccggaca tttgttgtaa gatcttccct aaagaaaata atgatttttt
     1321 atacatagtt gttgtgtaca gaaatgacag ccctctagga gaacaacggg caaatagatt
     1381 tatagaatta tataatataa aaagagatat catgcaggaa ttaaattatg agttaccaga
     1441 gttaaaggca gtaaaatctg aaatgattat cgcacgtgaa atgggagaaa t
//
LOCUS       DAZGTR010000348         1490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGTR010000348 DAZGTR010000000
VERSION     DAZGTR010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.351"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1490)
                     /locus_tag="ACHLCY_005299"
     CDS             complement(<1..>1490)
                     /locus_tag="ACHLCY_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0372309.1"
                     /translation="ATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTLTAW
                     PDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQHRLVFYTRIQ
                     HGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEMTWYGWDGDRLTTVQTD
                     TTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRL
                     LDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLA
                     LISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYDPLQ
                     GRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSIDITG
                     SGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKSLPT
                     SGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASV"
BASE COUNT          292 a          476 c          364 g          358 t
ORIGIN      
        1 actgaagcgt caacacctgc accgatacca acagattctg tcgatgattc tggattatca
       61 acatccacta cataagtata cgcaaaatgc cccccaactc cgccgccaac cgttacacct
      121 actgaattgc tgactcccga tgtaggtaaa gacttggtct cagaatatgt gatagccgca
      181 tttatcccac caccaattcc tgcatgtccg catgctgtag cataaataca aatatcagat
      241 aacatttcac ctttcttttt agcaaacgca acgccaagtc ctgcatgacc acctaatgat
      301 attagatctc cactgccagt aatatcaatt gatgatgttc catatgaatc atcatgccaa
      361 acatcatata ataattgata taaattatac aaacccaaag gatccatcct tattatcgga
      421 ttcagtgggt aattatacag gttccatccc cccgccagcc caatggggtc ttgtgtaata
      481 tacctcccct gtagcgggtc atagtaccgg ttccggttat agtacagccc tgattcctca
      541 tcatgctgct gccccggcag acggtatggc tggtgcaggt aatacgggtt ctcttcatta
      601 agctggttgc cccattcatc atactccccg cgccacgccg tattgccgtc ttcgctgatg
      661 agcgccagcg gcaggccccg gtggtcgcag tggtaaaaat gaacttttcg cgccggtgtg
      721 tattccggct ccacctgtct ggccagttgc tccaccgtca gcccgcactg cgcaagccac
      781 gcccggcttt cactgctcac gcggtctgcc cggatttctt cctccagcct gtccagcagc
      841 cgcaccagtt cagccggaaa taccacgccg tgcccgtcct cgctcccttc ctgctgcagc
      901 ttctccgcca ggctgcggcg ctgcgctttc tcccgctcgc cgttgtctgt ttcgatgcgg
      961 atgagcggcg cgaagctccc cggctgatat accgtctgga tacgcgtggt gtcggtctgt
     1021 accgtcgtca gccggtcgcc gtcccacccg taccacgtca tctccggttt acgcgacagc
     1081 gacatccacc ccgtcaggtc acgctcccgc cgccataccc gtttcgccat tcgccgtccc
     1141 agcgggtcgt agaggtagcg gctctcgacc agtggctcgc catgctgtat ccgcgtgtag
     1201 aacaccagac ggtgctggct gtcgtagtgg tagtggtggg tccgctcgtc gtccgtccgg
     1261 ataacacccg ccgggataag gtccgtcttc tccgtcagtc tgccgtattc atcatagtgg
     1321 taaacatagt gcgcatcctc tgcgatgcgg ttatccggcc acgccgtgag ggtactgtcc
     1381 gggtgcagct ccgggtccgg caggcggttt cccgccgggt cggtggcata cgggatgcgg
     1441 atgtccaggt ccggtgcgag ggtacgcacg ctctccagcc tgcccgtggc
//
LOCUS       DAZGTR010000349         1470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGTR010000349 DAZGTR010000000
VERSION     DAZGTR010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.352"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..611
                     /locus_tag="ACHLCY_005300"
     CDS             48..611
                     /locus_tag="ACHLCY_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0372310.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREVAAIPQPDENNPEMPPPAVIS
                     HGIEYMEDGLPDPVKAMGQILVENLIIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNQNEESG"
     gene            608..>1470
                     /locus_tag="ACHLCY_005301"
     CDS             608..>1470
                     /locus_tag="ACHLCY_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0372311.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAAWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEA"
BASE COUNT          358 a          376 c          446 g          290 t
ORIGIN      
        1 ggctgaacca tacccggctg agtgcgttat ggacccggga ggaggctgtg ctgacaacgc
       61 aaaaacgaaa atttgcgctg gcgctcatgt ccgggaaaaa caaaacagcg tcagccattg
      121 ccgccggtta ttcggcgaag accgccaggg ttaaaggctc gcagctggca aaagatccgg
      181 aggtgctcgc gtttatagcc cgtaaacagt gcgaaacggt ggaggtggat gaggttcctg
      241 tttaccggca gaaaaaatca gagcaggagg ataaaccccg tcgccgtgag gtggctgcaa
      301 taccacagcc ggacgaaaac aatccggaga tgcctccgcc cgcggtgata tctcatggta
      361 ttgaatatat ggaggatggt cttcccgatc cggtgaaagc tatggggcag atcctggtgg
      421 aaaacctgat aattgacccg aaactggcac tggatgcggc ctggcgactg gcgcagttca
      481 cacaccataa aaaaggcgat gccggtaaaa aatcggcaaa aggtgatgcc gcgaaaaaag
      541 cggctaaccg ttttgcggtg ccaccacctc cccgactggt ggtgaataac cagaatgagg
      601 aaagcggatg atacctgtat ggagcacggc atgtccggac tgggcagagc gcctgaaaaa
      661 ggggctgtcg attattccgg atccgattta tccggacgag gccgcacatg ccctggcgat
      721 ttttaaacaa ctgcggattg tggatgcacc tggtagcccg acattcgggg agtcctgtgc
      781 agcgtgggtg tttgacctgg tggcggccct gtttggctcc tatgatgcgc agaccggtgt
      841 acgccatatc aaggaagtgt ttatccttat ccccaagaaa aactcgaagt ccacgctggc
      901 cgcggggatc atgatgacgg cgctgttact gaactggcgg caggcggcgg gttacacgat
      961 tctggccccg actgtggagg tggcggccaa cgccttcaac cctgcccggg atatggtacg
     1021 acgtgacgat gatctggatg acctctgcca ggtacagacc catatccgga ccatcaccca
     1081 cagggtgaca gacaccaccc tgaaggtggt ggcagccgat ccgaatacgg tgtccggtat
     1141 caagtccgtg gggacactga ttgatgaact gtggctgttt ggcaagcagt acaaagcgga
     1201 agacatgcta cgtgaagcca tcggcgggct tgcctcccgt ccggaaggat ttgtggtgta
     1261 cacaaccacc cagtcgaatg aaccgcctgc cggggtgttc agacagaaac tgcagtacgc
     1321 ccgggatgtc cgtgacggca aaattcatga tccgcacttt ctgccggtga tatttgaaca
     1381 ccctcctgaa atggtggaaa gcggggctca cctgctgatg gaaaacctcg ccatggtcaa
     1441 tccgaatctc ggttattcgg tggatgaggc
//
LOCUS       DAZGTR010000350         1450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGTR010000350 DAZGTR010000000
VERSION     DAZGTR010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.353"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            222..>1450
                     /gene="espK"
                     /locus_tag="ACHLCY_005302"
     CDS             222..>1450
                     /gene="espK"
                     /locus_tag="ACHLCY_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspK"
                     /protein_id="HFP0372312.1"
                     /translation="MLPTSQLRPTGTFCSYSAETSADIKSEITPIQIEEARASGRLYI
                     KDCDIEYLPQLPNEITSVTIENCNNLTTLTGLPVNTQNLSVINCEKLQITDMPSTVKN
                     LHIELTDSPFIHFISEGIECLRVCHCYISGVPESVRYLEIKGSATDSIKNVPNGLSSL
                     SINSYNPENQARIDNLISPSLKTLSLTGCSNIILPEKLPESVTSVTIHAEQKTTWNIG
                     VEGMPDGLDLDLQNVLLSPDVVKAKNITFQGNALDVALHFREGDIVYGLSSPREKLVN
                     SIKLVNDFSKKDIITQNTLTNAVWDPRTPRKYKQDPLIKRALNEHERGIKFKQHLKNH
                     NNYNVTMADLSVYNRDKLWAKTSKAGLEFQTLTRNKTVIFCADELVNSLKLIANKSDG
                     YGQSITASELRWIYRNK"
BASE COUNT          505 a          305 c          271 g          369 t
ORIGIN      
        1 aaaaaacaac aaatggtata aaaatgagca atcctaatcc atgcatgacg gactggagaa
       61 agtattcgca aaagttaaaa tcaacagtct gttggttttg agatataact ctcttttcta
      121 atattgacac cttggctttg ctggttgtta aaattagctt gttgattagt agtatggata
      181 gaacttactc tgttatttat ttctaaaaat ggaatttatc aatgcttcct acatcgcaat
      241 tacgaccgac cgggacattc tgctcctatt ccgctgaaac atcagcagac atcaaaagcg
      301 aaatcacacc aattcagata gaagaagcgc gggccagtgg tcgtttatat atcaaagatt
      361 gtgatattga gtatctgcca cagttaccaa acgaaataac atcagttaca atcgaaaact
      421 gcaacaacct gacaaccctt acaggattgc cggttaatac acaaaacctc tccgtcatta
      481 actgtgaaaa attacaaatc acagacatgc catcaaccgt aaaaaatcta catattgaat
      541 taactgattc accatttata catttcatat ctgaaggcat cgagtgcctg agggtttgcc
      601 actgctatat atctggagtg ccagagagtg tccgctacct tgaaataaaa ggtagcgcca
      661 cagacagcat aaaaaatgtt ccaaacgggt tatcatctct cagcatcaat agctataatc
      721 cggagaatca ggccagaatt gacaacctga tatcaccgtc actgaagacg ctatcgctga
      781 ctggatgtag caatattata ctgccggaga aacttccgga gagtgtgaca tcggtaacca
      841 ttcatgcgga gcagaaaacc acgtggaaca tcggtgttga agggatgcct gatgggctgg
      901 atcttgattt acaaaatgta ctactctctc cagatgtagt taaagcaaaa aacatcacct
      961 ttcagggcaa cgctctggat gtggccttac actttcgcga gggagacatt gtctatggac
     1021 tatcttcacc cagagaaaaa cttgtcaaca gcattaaact agttaacgac ttttccaaaa
     1081 aagatattat aactcagaat acgttaacaa acgcagtatg ggaccccaga acacctcgca
     1141 aatataagca agatccactt atcaaaagag cattaaatga acacgaaaga gggataaaat
     1201 ttaaacaaca cttaaagaat cacaataatt ataatgttac catggccgac ctttccgtat
     1261 acaatcgcga caaattatgg gcaaaaacaa gcaaggccgg cctagagttt cagacattaa
     1321 cacgcaataa aacggttatt ttttgtgcgg atgagcttgt caactcactc aaactcatag
     1381 ctaacaagtc agatggctat ggccagagta ttaccgccag cgaattacga tggatttacc
     1441 gtaataaaga
//
LOCUS       DAZGTR010000351         1450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGTR010000351 DAZGTR010000000
VERSION     DAZGTR010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.354"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..926
                     /gene="katP"
                     /locus_tag="ACHLCY_005303"
     CDS             <1..926
                     /gene="katP"
                     /locus_tag="ACHLCY_005303"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catalase/peroxidase KatP"
                     /protein_id="HFP0372313.1"
                     /translation="FIWQDPLPAADYTMIDGKDIKSLKEQVMDLGIPASELIKTAWAS
                     ASTFRVTDYRGGNNGARIRLQPEINWEVNEPEKLKKVLASLTSLQREFNKKQSDGKKV
                     SLADLIVLSGNAAIEDAARKAGVELEIPFTPGRTDASQEQTDVASFSVLEPTADGFRN
                     YYSKSRSHISPVESLIDKASQLDLTVPEMTALLGGLRVMDINTNNSSLGVFTDTPGVL
                     DNKFFVNLLDMSTRWSKADKEDTYNGFDRKTGALKWKASSVDLIFSSNPELRAVAEVY
                     ASDDARNKFIHDFVKSWNKVMNSDRFDLNNK"
     gene            970..1359
                     /locus_tag="ACHLCY_005304"
     CDS             970..1359
                     /locus_tag="ACHLCY_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP0372314.1"
                     /translation="MQKSNKVIISCGMALYFLCGGITVAEELDESMQVLARNMSVLQS
                     SMDKKELLEALDRMDDAVDESMKRLPESISPADEEGKNDYIKELQKLEKEIMLAKQKV
                     LSGQLSDIPDSVNKMNKIRVEGHDKFR"
BASE COUNT          461 a          246 c          359 g          384 t
ORIGIN      
        1 catttatctg gcaggatcct cttcctgcgg cggattatac aatgattgat ggtaaagaca
       61 ttaagtcgct gaaagagcag gttatggatt tgggtatccc tgcatctgag ctgataaaga
      121 cagcctgggc ttcagcttcc acatttcgtg tgactgatta tcgtggggga aataatggtg
      181 cccgcatcag gttacagccc gaaattaact gggaagttaa tgagcctgaa aaactgaaga
      241 aagtactggc atccctgacc tcattacagc gtgaatttaa caaaaaacag tctgacggaa
      301 agaaagtgtc gttggctgat ttaattgttc tttcgggtaa tgctgcaatc gaagatgcgg
      361 ccagaaaagc cggggtggaa cttgagattc cctttactcc gggaagaact gacgcctctc
      421 aggagcagac ggatgttgcc tcattcagtg tactggagcc gacagcagat ggattcagaa
      481 attattactc aaaaagcaga agtcatatat cgccggttga aagcctcatt gataaagcca
      541 gtcagctgga tctcaccgtt cctgaaatga cggcattact gggtggtctg cgggtaatgg
      601 atattaatac aaataattct tcgttgggag tgtttaccga tacccctggt gttctggata
      661 acaagttttt tgttaatctg ctggatatgt caacacgatg gagtaaagca gataaagaag
      721 atacatacaa tggattcgat cgtaaaacgg gagcattaaa atggaaagca tcctctgttg
      781 atttaatctt cagttcaaat cctgaattac gtgcggtggc agaagtatat gcctcggatg
      841 atgcgagaaa taagtttatt catgattttg ttaaatcgtg gaataaagtt atgaatagcg
      901 atcggtttga tttaaacaat aaatgataaa actgatgagg gagggcacct ccctcatgaa
      961 ggagaaatta tgcagaaaag caataaagtt ataatcagct gtggtatggc attatatttt
     1021 ttgtgtgggg ggattacagt ggcagaagaa cttgatgaaa gcatgcaagt tcttgcaagg
     1081 aatatgagtg tacttcagtc atcaatggat aaaaaggagc ttcttgaagc tcttgacaga
     1141 atggatgatg ctgtagatga gtcaatgaag cgtcttcctg agagcatttc accggctgat
     1201 gaagaaggga agaatgacta tataaaagaa cttcaaaagc ttgaaaaaga gattatgctg
     1261 gcgaaacaaa aagtcttatc gggacagtta tcagatatac ccgattcggt aaacaagatg
     1321 aataaaatca gagttgaggg gcatgataaa ttcagataaa tcagttggtt gattcctgcg
     1381 ggacgaatac atacgtcagg aacataactt tatggcagtg aaccgccccg ggaatcctgg
     1441 agactaaact
//
LOCUS       DAZGTR010000352         1439 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGTR010000352 DAZGTR010000000
VERSION     DAZGTR010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.355"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHLCY_005305"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHLCY_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP0372315.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQHALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          430 a          265 c          287 g          457 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacatgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgc
//
LOCUS       DAZGTR010000353         1427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGTR010000353 DAZGTR010000000
VERSION     DAZGTR010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.356"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..637)
                     /locus_tag="ACHLCY_005306"
     CDS             complement(<1..637)
                     /locus_tag="ACHLCY_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP0372316.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STPSTGYQPLILQSIKTKLLSSRLNKFNDFEDSFFNGLGISVHDISAQKISL"
     gene            818..970
                     /locus_tag="ACHLCY_005307"
                     /pseudo
     CDS             818..970
                     /locus_tag="ACHLCY_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          459 a          224 c          261 g          483 t
ORIGIN      
        1 aaagagatat tttttgagct gaaatatcat gaacactgat tcctaagcca ttaaaaaaac
       61 tgtcctcgaa gtcattaaat ttatttagtc ttgatgaaag taatttggtt ttaatgcttt
      121 gcaggataag aggttggtat cctgtcgagg gagttgaatt aatgccattt tcgatacatt
      181 ctttgattat tcgtattgga ctattataac catatttatt tattttttca tgaaaagata
      241 aatttaattg ttggctatga aaattgctgc catttccata tctaaaatgt tcaatcagat
      301 cttcgataag tgttttatat tgtccaaaaa aggaaagcat ttttgctagt tctttcattt
      361 ctgcaaataa aatatcagtg cattcacgtt gtgatatttt tgttccggaa cttgaggcac
      421 taaaagcaac attaatagaa cctgggccag ggaaatggta tttaataagt ctgtaaggat
      481 caacttttgc agagatatcg cttaggccga gtgagagaaa gtcttgttct accatatcac
      541 catattgcat atcatcagca gaaaaatcat cgaataaact aatagtttcg aaaagtgtac
      601 agggaatatt aattggcgga agtaatatgt tattcatttg catttgcctt ggttatattt
      661 aagctcaata tcaattcgag aacaaatgct agggtaaaaa aatatttaaa aaagaagtaa
      721 acttcacatt ttttcattac atctctgaag agaacctcgt taatgattgg tcttgctcat
      781 tgttaataac aaaatgttga atttaccgat taggaaactt tttcctacgc ggattgcctc
      841 cagctatggc atgaactttg agtttatgcc agaactgaag tggggcttcg gctaccctgg
      901 cgcgattatc tttatgatcc tcgcgggcct gacaccgtat ctgtacttta agcgtaagaa
      961 ctggctgtaa ctatacgatg tcgctgtgag taagttagtg tgctgacagc gacattttag
     1021 ccgagggaat cgcagataac ataattgttg tgaataatat ccatgccacg ttgagttgga
     1081 cgcttaatga ctcacatctc accccttcaa ttgctgcaga gataatagaa aaaagtgtca
     1141 agcccaatga aatcaataca aagtagattg ccttacatcc agtatctctt attctttttg
     1201 ctccaatatt gatatttaca taggtattat taataaccag aataaaactc aaagccaggt
     1261 tgatatcatt caaagttatt aatattgacc atgatgttaa tgtgtagatt atgaactgaa
     1321 agcgtgataa acgctcaagc ctggatgggt agaatatatt tctgatggat gatttgaaca
     1381 tttttacatt gttttatata taattgtttt gaatatatat tacctga
//
LOCUS       DAZGTR010000354         1414 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGTR010000354 DAZGTR010000000
VERSION     DAZGTR010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.358"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            103..507
                     /gene="stpA"
                     /locus_tag="ACHLCY_005308"
     CDS             103..507
                     /gene="stpA"
                     /locus_tag="ACHLCY_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP0372317.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(554..1078)
                     /gene="ygaP"
                     /locus_tag="ACHLCY_005309"
     CDS             complement(554..1078)
                     /gene="ygaP"
                     /locus_tag="ACHLCY_005309"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP0372318.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(1088..1387)
                     /locus_tag="ACHLCY_005310"
     CDS             complement(1088..1387)
                     /locus_tag="ACHLCY_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0372319.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          381 a          327 c          335 g          371 t
ORIGIN      
        1 aggactgtaa atagattaaa ttttgtggaa atataataag tgatcgctta cactacgcga
       61 cgaaatactt ttttgttttg gcgttaaaag gttttcttta ttatgtccgt aatgttacaa
      121 agtttaaata acattcgcac tctccgtgcg atggctcgcg aattctccat tgacgttctt
      181 gaagaaatgc tcgaaaaatt cagggttgtc actaaagaaa gacgtgaaga agaagaacag
      241 cagcagcgtg aactggctga gcgtcaggaa aaaattagca cctggctgga gctgatgaag
      301 gctgacggaa ttaacccgga agagttatta ggtaatagct ctgctgctgc accacgcgct
      361 ggtaaaaaac gccagccgcg tccggcgaaa tataaattca ccgatgttaa cggtgaaact
      421 aaaacctgga ccggtcaggg ccgtacaccg aagccaattg ctcaggcgct ggcagaaggt
      481 aaatctctcg acgatttcct gatctaatta ctgaggccgg atgtcgctgc tagggcgcgt
      541 ccggcgtcgc ttctcaagtt cgttggttcc acggcatctt atctaacaac cttgccattc
      601 cacaaaaacc gctgattcct gcaaacagta acccggcacc aacaaagcca cttaataaga
      661 agaaaccgct atttacggta taacccagta caacgccgat taatattaaa ccacccgcag
      721 cgatctgcac ctggcgcatt aacggcaagg gttgagattt atttaccgct actggcaatc
      781 cagctctttt ccagccatca atcccatctt cgagtaaaaa tatttctgcg ggggcggcaa
      841 tcgctgctaa tttatcggcg ttattactgg tgcgtttacc tgcctggcag tggaatataa
      901 tttgctcgcg acgtaattta ggtggaagac ctgactgttc cagcacggat aatggagcca
      961 gatctgcttc aggaatatgt tcacgaagat attcatcagc gtcacgaata tcgattaact
     1021 ttgcgccgcg tgcgattaat tcttgtgcat catgcggcga aatggttgtc aaagccatag
     1081 tgactcctta cggacaatag acatttttca gggtggcgat aatggcattt accgcctcat
     1141 ttttaatgga atatagaatg cgttgggcat cccgttggct gtcgataagc ccttcgtccc
     1201 gcatacgagc gagatgctgt gatgtcgcag aggcactcag tccggtaatg cgcgtcagct
     1261 ctcccgcgct ggtgccgggg gaaccgctaa gcatgcacag aatcagcaat cgtttaggat
     1321 ggctcattgc tttcaataag gccgctgcct gttcggcact ggcctgtaat tgcgcgagtt
     1381 cagtcatatt tatttaagta ttttctaaat taag
//
LOCUS       DAZGTR010000355         1412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGTR010000355 DAZGTR010000000
VERSION     DAZGTR010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.359"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..138
                     /locus_tag="ACHLCY_005311"
     CDS             <1..138
                     /locus_tag="ACHLCY_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0372320.1"
                     /translation="NMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGKVVVIGRQ
                     A"
     gene            complement(329..856)
                     /gene="sodC"
                     /locus_tag="ACHLCY_005312"
     CDS             complement(329..856)
                     /gene="sodC"
                     /locus_tag="ACHLCY_005312"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0372321.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            990..>1412
                     /locus_tag="ACHLCY_005313"
     CDS             990..>1412
                     /locus_tag="ACHLCY_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372322.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQL"
BASE COUNT          340 a          343 c          415 g          314 t
ORIGIN      
        1 aacatgattg cccagggtaa cccgatgccg gtgccttacg gggaaatgct ggttggctcc
       61 cgccgtatat cccaggacat cagtacccgt gatgaaggcg ggggcggaaa ggtcgtggtt
      121 atcgggcggc aggcataaaa gcgaaaaaat cccgcagtgc tcacggacag gaactgcggg
      181 agcgttacga agattgagtg taaggaatta ttcttatgtc acgacaaaaa cattaactca
      241 gagagggagg atgtgccgtt cttttcaggg agaaaggatt tatcgtcctg aggaataaag
      301 gtaaggggcc cgccccttac ctgactgatt attgaatgat gccgcaggcc attctcgcac
      361 caccaccgcc caggggctcc ggatggtcat gatggttatc accgccagca tgaagcatga
      421 gagaacgccc tttaatctct tttaatgagt tcagtctcgg ggccaggacc gggtagttcg
      481 cttttccgtc atgcgtcacg aacagcgcag ggaggtcgcc caggtgtcca tccggagacc
      541 aggggccaag atgtttgccg gtgtttttcg ggtcaaagtg accgccagcc gataatgctg
      601 cgaccggttt tccgtctttc agtgccgggg cgcaatttcc tttttcgtgc acatgaaaac
      661 catgaatgcc ttcagacaga gagtgaaggg ctggtgtgaa cagcagaccg tagggggtct
      721 cctgaatggt tatttttcca atgctgactt cttttccgtc agcactgaca aggttcattg
      781 ggacttcctg ttctgctgcg tatccgcatg atgctgctgt cagcatggca atggcagcaa
      841 tgattttaca tttcataaaa ccctcattaa ttccgttaac agactgagct tgctggttac
      901 agggtaacaa acagcgttct gatgatatcg cgcaatagct gtgcaatatc ctatcactgc
      961 gattaataat accaattgag aggaacatta tgggcaaagg tggcggcagg gcgcacacgc
     1021 ctcgagaggc gaaggataat ctcaaatcca cgcagatgat gagtgtgatt gatgcgattg
     1081 gtgagggacc gatagaaggc ccggtgaagg ggctgcagag tatcctggtg aacaaaaccc
     1141 cgctgacgga cacggacggt aatcccgtga tacacggtgt gactgcggtc tggcgtgccg
     1201 gggagcagga gcagacacca ccggaaggct ttgagtcctc cggagctgaa accggactgg
     1261 gcgtggaagt gacgaaggca aagccggtga cgcgcaccat tacgtccgcg aacattgacc
     1321 gcctgcgggt taccttcggg gtgcagtcac tggtggagac cacctcaaag ggtgaccgta
     1381 acccgacgtc tgtccgcctg ctgattcagc tt
//
LOCUS       DAZGTR010000356         1396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGTR010000356 DAZGTR010000000
VERSION     DAZGTR010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.360"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1295
                     /gene="pilV"
                     /locus_tag="ACHLCY_005314"
     CDS             <1..1295
                     /gene="pilV"
                     /locus_tag="ACHLCY_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFP0372323.1"
                     /translation="SIRYDKISTLSSSNNQSSDPGPRTCSAAGCEITYQTLINEGLLP
                     VGYTGTNAQKASYKIVLKRSGTAPDYVINGLITTASPWEEGGRIRYDLLGKAVQAAGV
                     DGGMSRNTKIASGYGGQWSENSNSYSNITGGGLLAYRVGYNSSMYSVYLRRDGTLPMT
                     DDLNLGGKSIKNIKDITASGTTTTGALKTTGNASVTSALTVGGTSTLNGPVNINNNLK
                     VKSDTYLNTLSTTGLAKFGSRIATNGLNPNDLPAGWAGGVRTYDLYASGTVGVGTGKT
                     VKVYMNNAGNIYASGNLTAGTIISNGTIESTGRIKAGEYLHLNVQATLNAKCTPNGLV
                     GRDSTGRVLSCVNGKWQTASGDGLKGIFITITDQISGYKCVIPNSDTGTCACPGSTYS
                     PQFGYISGTLIAEYNEERCSGGRNTHCYSNFRRLYACR"
BASE COUNT          413 a          258 c          352 g          373 t
ORIGIN      
        1 tcagtattcg ctatgacaaa atttccacac tgtcatcatc caacaatcaa agtagcgatc
       61 cggggccaag aacatgttcg gctgctggtt gtgaaatcac ttaccagacg ttaattaatg
      121 aaggtctgtt gcctgtcggc tatacaggaa ctaacgcgca aaaagcatcc tacaagattg
      181 tgttaaagcg ttcaggaaca gcacctgatt atgtcatcaa tggtcttata acaaccgcca
      241 gcccgtggga agaaggtggt cgtattcgtt atgacctgtt aggtaaagcg gttcaggcgg
      301 ctggtgttga tggtgggatg agcagaaaca ccaagatagc gtccggctat ggtggtcagt
      361 ggagtgagaa ctctaatagc tacagcaata tcacgggtgg tggattgctg gcttaccgcg
      421 tggggtataa ctcttcaatg tattctgttt atctgcgccg tgatggaacg ttgccgatga
      481 cggatgatct gaatcttggt gggaagagta tcaaaaacat caaagacatt acggcatcag
      541 ggacaacaac aaccggcgca cttaagacaa cgggtaatgc gtctgttaca tcagctctga
      601 ctgtgggagg aacctcaacc cttaatggtc ctgtaaatat caacaacaac ctgaaagtaa
      661 aatctgatac ctatcttaat acgctatcaa caacaggtct ggcgaagttt ggtagtcgta
      721 tcgcgacgaa cggacttaat ccaaacgatt tgcctgctgg ctgggctggc ggtgttcgca
      781 cctatgattt gtatgcgtca gggactgttg gtgtaggaac cgggaaaaca gttaaagttt
      841 acatgaacaa tgcaggtaat atttatgctt caggtaatct taccgcaggc acgataatat
      901 ctaacgggac aattgaatca acaggcagga tcaaagcagg tgaatatctc catttgaatg
      961 ttcaggcaac attaaatgcg aaatgtacgc ctaatggtct ggtggggcga gacagtacag
     1021 gtcgagtgtt atcgtgtgtt aacggtaaat ggcagacagc aagtggtgat ggcctgaaag
     1081 gtatttttat aacgataaca gatcaaatct ctggttataa atgcgttata ccgaatagtg
     1141 atactggaac atgtgcctgt ccgggatcaa cgtatagtcc tcagtttggt tatatatctg
     1201 gaacccttat tgcagaatat aatgaggagc gttgtagtgg tggtaggaat acacattgtt
     1261 atagtaattt cagaaggtta tatgcatgta ggtaatgcta cttactgaaa attaatattt
     1321 gacaagtatg gcgagattaa aggagtattc tatcctatag atagttcctc tatagaggat
     1381 gtaaaagggc catgtg
//
LOCUS       DAZGTR010000357         1384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGTR010000357 DAZGTR010000000
VERSION     DAZGTR010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.361"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1384
                     /locus_tag="ACHLCY_005315"
     rRNA            <1..>1384
                     /locus_tag="ACHLCY_005315"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          347 a          313 c          428 g          296 t
ORIGIN      
        1 atgttggccg ggcgacggtt gtcccggttt aagcgtgtag gctggttttc caggcaaatc
       61 cggaaaacta aggctgaggc gtgatgacga ggcactacgg tgctgaagca acaaatgccc
      121 tgcttccagg aaaagcctct aagcatcagg taacatcaaa tcgtacccca aaccgacaca
      181 ggtggtcagg tagagaatac caaggcgctt gagagaactc gggtgaagga actaggcaaa
      241 atggtgccgt aacttcggga gaaggcacgc tgatatgtag gtgaagtccc tcgcggatgg
      301 agctgaaatc agtcgaagat accagctggc tgcaactgtt tattaaaaac acagcactgt
      361 gcaaacacga aagtggacgt atacggtgtg acgcctgccc ggtgccggaa ggttaattga
      421 tggggtcagc gcaagcgaag ctcttgatcg aagccccggt aaacggcggc cgtaactata
      481 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg aatggcgtaa
      541 tgatggccag gctgtctcca cccgagactc agtgaaattg aactcgctgt gaagatgcag
      601 tgtacccgcg gcaagacgga aagaccccgt gaacctttac tatagcttga cactgaacat
      661 tgagccttga tgtgtaggat aggtgggagg ctttgaagtg tggacgccag tctgcatgga
      721 gccgaccttg aaataccacc ctttaatgtt tgatgttcta acgttgaccc gtaatccggg
      781 ttgcggacag tgtctggtgg gtagtttgac tggggcggtc tcctcctaaa gagtaacgga
      841 ggagcacgaa ggttggctaa tcctggtcgg acatcaggag gttagtgcaa tggcataagc
      901 cagcttgact gcgagcgtga cggcgcgagc aggtgcgaaa gcaggtcata gtgatccggt
      961 ggttctgaat ggaagggcca tcgctcaacg gataaaaggt actccgggga taacaggctg
     1021 ataccgccca agagttcata tcgacggcgg tgtttggcac ctcgatgtcg gctcatcaca
     1081 tcctggggct gaagtaggtc ccaagggtat ggctgttcgc catttaaagt ggtacgcgag
     1141 ctgggtttag aacgtcgtga gacagttcgg tccctatctg ccgtgggcgc tggagaactg
     1201 aggggggctg ctcctagtac gagaggaccg gagtggacgc atcactggtg ttcgggttgt
     1261 catgccaatg gcactgcccg gtagctaaat gcggaagaga taagtgctga aagcatctaa
     1321 gcacgaaact tgccccgaga tgagttctcc ctgactcctt gagagtcctg aaggaacgtt
     1381 gaag
//
LOCUS       DAZGTR010000358         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGTR010000358 DAZGTR010000000
VERSION     DAZGTR010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.362"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            202..636
                     /locus_tag="ACHLCY_005316"
                     /pseudo
     CDS             202..636
                     /locus_tag="ACHLCY_005316"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            671..1231
                     /locus_tag="ACHLCY_005317"
     CDS             671..1231
                     /locus_tag="ACHLCY_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFP0372324.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTVGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            1238..>1372
                     /locus_tag="ACHLCY_005318"
     CDS             1238..>1372
                     /locus_tag="ACHLCY_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFP0372325.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     G"
BASE COUNT          363 a          314 c          461 g          234 t
ORIGIN      
        1 gccaggggaa aaggcggtgg cagtggtccg gttgattacc tgctgggccg ggagcgtagc
       61 cgggaagggg cgagagttct gcggggtgct cccgaagagg tgcgggaact catcgatgcc
      121 acgccgtttg cgaaaaaata cacctcgggg gttctgtctt ttgcagagca gacactgcca
      181 ccgggagagc gtgaaagggt gatggagagc tttgagcggg tactgatgcc cggtcttgaa
      241 aaaaatcagt acagcatcct gtgggtggag caccaggaca aggggcgtct tgagctgaat
      301 tttgtcatcc cgaatatgga gctggcgagc ggaaaacggc tccagcccta ttacgaccgg
      361 gcggaccgtc cgcgaatcaa tgcctggcag acactggtga atcaccatta cgggctgcac
      421 gacccgaacg caccggagaa caggcggaca cttgtcaccc cgaataatct gccgaaagcg
      481 aaacaggagg ccgcagaagc gattacgagg ggactggagg ctctttacca tgccggagcg
      541 ctaaaaacgc gtcaggatgt cactgaggcg cttacagcgg caggttttga ggtggtgagg
      601 acaaccagga gcagtatcag tatcgccgac ccggagggcg ggcgtaatct caggctgaaa
      661 ggggcgctgt atgagcaatc ttttacaaat ggggacagac ttcgagaaga aactgaaaga
      721 gcgtgccgtc tctacagaga acatgctgaa cagcgagttc aggaagctcg aagcgtctgt
      781 agacagggaa ctgaacttaa acgggcagaa aatcagagac gctatcagcg cacacacagc
      841 agcggtgaaa gaacaactgg agacgctgaa cagtgccgtg aacgcgcagt tcagcgccac
      901 agagaacgaa cttgcccggc ggcaggaaga gctgctctgg aagctggtca aagggcggat
      961 actgtacccg tcgctgacgg cgctttgcgt cacggtcggg atttttctgg cgtcatgggg
     1021 gctgattcag tggcaggaaa gccgtattgc ggcgaacatc ctggctatca gggaccagga
     1081 agagacgctg gcgaaactcc gggaaaaaac atggggtgtg acgtatcacg agggcaggaa
     1141 cgggaaattt ctggtgttgc cgtccggggt gaagggggaa aacaactgga cggtggagaa
     1201 gaagaacgca gtaagactgg tcagggagta agtgagtatg acagagctgg aaatgcactt
     1261 actgaacgcc ttagagcaat tgcagcagga ctatacgcag cggctgaaag actgggagag
     1321 cgccttcacg gatttgcagc atatgttcga gctgacacga caggagaacg gg
//
LOCUS       DAZGTR010000359         1370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGTR010000359 DAZGTR010000000
VERSION     DAZGTR010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.363"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>1370)
                     /locus_tag="ACHLCY_005319"
     CDS             complement(<1..>1370)
                     /locus_tag="ACHLCY_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0372326.1"
                     /translation="SRMGSEYYRTLQNGGRQAAAAARDQRRALAELNSQLTEIRGSAV
                     GMAGAFAGAFATGHLISLADEWSSVNARLKQASQSSDEFSSSQKVLMDISQRTGTAFS
                     DNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGASTAEAGSVITQFSQALAQG
                     VLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMADDGQLTADKVVPALISQLEVLR
                     DEYAAMPETVSDGITKVENAFMAWVGGANEASGVTKTLSGVLNGVAGQIDNVATAVGA
                     LVAVGVARYFGNMASGAMSATAGLVTAARNEVALAEAQFRGTQIATARARAAVYRAQQ
                     AVAAARGTEMQIAAEARLAATQERLNRNIAARSAAQNALNSTTAVGSRLMSGALGLVG
                     GVPGLVMLGAAAWYTLYQNQEQARESARQYALTIDEIAHKTPSMSLPEASDNEGRTRA
                     ALTEQNR"
BASE COUNT          270 a          470 c          338 g          292 t
ORIGIN      
        1 cggttctgct ctgtcagcgc cgcccgtgtt cgtccttcat tatctgaggc ttcaggcaaa
       61 gacattgacg gcgttttatg cgcgatttca tctatcgtca gtgcatactg gcgcgcagac
      121 tccctggcct gctcctgatt ctggtacagc gtgtaccatg ctgcagcccc cagcatcacc
      181 agtccgggta cgccaccaac cagccccagc gcaccgctca tcagacgtga gcccaccgcc
      241 gttgtactgt tcagcgcatt ctgggcggcg cttctggcag caatatttct gttcaggcgt
      301 tcctgtgtgg ccgccagacg ggcttctgca gcaatctgca tctccgtccc gcgggctgcc
      361 gccacggcct gctgagcacg gtacacggct gctcttgccc gcgccgtggc aatctgcgtt
      421 cccctgaact gtgcttccgc cagtgcaact tcattacgtg cagccgtcac aagtcctgcc
      481 gtggcagaca tcgctccgga ggccatattg ccaaagtacc gggcaacccc gacggcaacc
      541 agtgccccca cggctgttgc cacattatca atctgtccgg caacaccgtt cagcacgccg
      601 gagagcgttt tcgtcacccc gctggcctca ttcgcaccac ccacccaggc cataaaggcg
      661 ttttccacct ttgtgatccc gtcagagacc gtttccggca tggccgcgta ttcatcacgc
      721 aatacctcca gctggctgat taacgcagga acgactttat ccgccgtcag ttgaccatcg
      781 tccgccatcg ccttcagatc tttacgggcc acgcccatac ccgcagccag tgcacgtacg
      841 atccggtcac cactttcatt gaccgaatta aattcctcac cgcgcaacac accctgtgcc
      901 agcgcctggc tgaactgggt gatcaccgaa cccgcctcag ccgtactggc accggagatt
      961 ttcagccccg tggaaatggc ctccgtcacc ttcagcacat catcagcact gtaaccatat
     1021 tcacgcattg aggctgccga gcgggcaaac agggccgcat tatccgaaaa agcggtacct
     1081 gtccgctgac tgatatccat cagcactttc tgtgatgacg aaaattcatc agatgactgc
     1141 gacgcctgtt tcagacgggc attcacggaa ctccactcat cagccagtga aatcaggtgt
     1201 ccggtggcaa aggcaccggc aaatgcgcca gccattccga cagcagaacc gcgaatttcc
     1261 gtcaactggc tgttcagttc tgccagggca cgtcgctgat cccgggctgc cgcagcggcc
     1321 tgacgcccgc cattctgcag ggtccggtaa tattcactgc ccatacggga
//
LOCUS       DAZGTR010000360         1367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGTR010000360 DAZGTR010000000
VERSION     DAZGTR010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.364"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            117..380
                     /locus_tag="ACHLCY_005320"
     CDS             117..380
                     /locus_tag="ACHLCY_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0372327.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            682..822
                     /locus_tag="ACHLCY_005321"
     CDS             682..822
                     /locus_tag="ACHLCY_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0372328.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
BASE COUNT          371 a          287 c          338 g          371 t
ORIGIN      
        1 ttatataaca tattgtaata ctgaaagaga gtgaggttat aagaacagac agttgaatct
       61 gaccgtctga atatgtacct ttatgcggaa ttttcactta aagggggaaa actgttgtga
      121 aaccgaatat ccatccggaa taccgtacag tagtatttca cgataccagt attgatgagt
      181 acttcaggat cggttcgacg atcaaaactg accgggtaat tgaactggat ggtgtaactt
      241 acccatatgt tacaattgac gtttcttcta agtcgcatcc gttttataca ggtaaactga
      301 gaactgtcgc ttcagaaggt aatgttgcac gctttaccca gcgttttggg cgttttgttg
      361 atgcgaaaaa aggtggctga ttttgccgcc agggtgcgtt tgacaattta aaaacgcacc
      421 agacagagag atcctgcacc tgaatatacc gttagcccgt cccaccgggg cgtgtgcaga
      481 tgatctcgtc accggaagat tgcggacctc tctgaaaacc agtacattac gcgcgggtgc
      541 ctttcggctg acggctggaa ggattacctg aaggccggat gtggaaaggc cccgagtcaa
      601 cttaaacgtt aacccgaggc cctgaccatc taccttcagc aagtggaagg ttagcgcctc
      661 tctgtaaaag gagcaagtgc tatgccgcaa aaaacgatca ttgtgggcat gttatgcctg
      721 accatgttgc tgaccgtctg ggtgttacac gcatcaccgt gtgaattcag ggtaagcttc
      781 atgtggtcgg aaattgcggc gttcttgcag tgtaaaccgt aagaaaccgc agcggggaca
      841 acagtccccg ctatccggtt gccaaaggtt cggtcgaaag tcgcaccctt gcacacaaat
      901 cacaaagaaa aaacaaaagt ccgcagtgta aaaactgtgg gcttttttat tcgcatcccg
      961 aaagtgtggt aacagcgtgg gggcaccaaa aaaagcccca taaaatgggg ctgaagattc
     1021 tgtaaggaat ttcacagcaa tgagattcct gctttctcca aggatatacg tgtcttcagg
     1081 ctgaatcaat gggggcgtgg ctctgaatcc acctgcgatt aaaacttgca caatgccggg
     1141 agttgagtta gaaaagtaca gggcgcgata tccggcttat tttgttgctg tgttttgcct
     1201 cgtgctggat gtcgcgtcca ccgtttttct gatgattcat atgaaatggt actttattga
     1261 tttactgcca ttctgatttg cctttttatg ttcattattg cggttgtcac ttccagtttc
     1321 tggtgatgtt ttgataaaag tgaaagaata aaacgataat tgcagaa
//
LOCUS       DAZGTR010000361         1353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGTR010000361 DAZGTR010000000
VERSION     DAZGTR010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.365"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..1193
                     /gene="espG"
                     /locus_tag="ACHLCY_005322"
     CDS             48..1193
                     /gene="espG"
                     /locus_tag="ACHLCY_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFP0372329.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLLKVLSLG
                     LWNPTYSRSERQSFQELLTVLEPVSPLPNELGRVSARFSDGSSLRISVTNSESIEAEI
                     RTPDNEKIFVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEIDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQMLLRDALG
                     LKDTHSSPSRNVIDHGISRHDAEQIARESSGSDNQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCTIDEMVRALQPRYAASETYLQNT"
BASE COUNT          448 a          283 c          246 g          376 t
ORIGIN      
        1 tacttgttgc caaattgttc attacaaacc agataggaga atctctcatg ataaatggac
       61 ttaataatga ctccgcatct ttagttttag atgctgcaat gaaagttaat tctgggttta
      121 aaaaaagctg ggatgagatg tcatgcgctg aaaagttact taaagtactt agtcttggtt
      181 tatggaatcc aacgtacagt cgtagtgaaa gacaatcatt tcaagagttg ttaaccgttt
      241 tagagcctgt atctccactt cccaatgaat taggcagagt atctgctcgt ttttcagatg
      301 gctcatcctt aagaatttcc gtcactaaca gcgaaagtat tgaagccgag attcgcacac
      361 cagataatga aaagattttt gtgctcctag agtcaaacga acaaaatagg ttattacaat
      421 ctttacccat cgatcgccac atgccataca ttcaggttca tcgtgcctta tctgagattg
      481 acctgactga tactacctca atgcgcaatc tacttggttt tacgtcaaaa ctatcaacaa
      541 ccttgattcc gcataatgct caaacagatc cgctttccgg gcctacacca ttcagctcta
      601 tctttatgga tacatgtcga ggactcggca atgcaaagct ttcactcaat ggtgttgata
      661 tacctgcaaa tgcacaaatg ttgcttcgcg atgcactagg acttaaagac acacactcat
      721 caccttcccg gaatgttata gatcatggta tttctcgcca tgatgcagag caaatagcaa
      781 gagaaagcag cggcagcgat aatcagaaag ctgaagttgt ggaattttta tgccatccag
      841 aagcagcaac ggccatatgc tcggctttct atcaatcttt caatgtgcca gccttaacgt
      901 tgacacatga aaggatctct aaagccagtg aatacaatgc ggaaagatcg ttagatacac
      961 ctaacgcttg cattaacata agtatctctc aatcatctga tggaaacatt tatgttacca
     1021 gccataccgg ggttctgata atggcgccag aagaccgccc caacgagatg ggcatgttga
     1081 cgaacaggac ttcttatgaa gtgccgcaag gtgtgaaatg tacaatcgat gaaatggtaa
     1141 gagcgctaca accaaggtat gctgcatctg aaacgtactt acaaaacact taaaataaaa
     1201 ctttattaat caaaaccaat aatagaaatt acacccacaa ttattaacaa ttcaccctga
     1261 agttaattac aattaaacaa agatatttgt tctgcaaaca atttacagta aatagagaaa
     1321 atgaaaagaa aaccattaat ttcatttttc att
//
LOCUS       DAZGTR010000362         1334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGTR010000362 DAZGTR010000000
VERSION     DAZGTR010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.366"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..280
                     /locus_tag="ACHLCY_005323"
     CDS             <1..280
                     /locus_tag="ACHLCY_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0372330.1"
                     /translation="NMEATGFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLH
                     HLPEQLSKRERYSEKWQRRTHSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            277..858
                     /locus_tag="ACHLCY_005324"
     CDS             277..858
                     /locus_tag="ACHLCY_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0372331.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGTVVVVGRQG"
BASE COUNT          318 a          319 c          406 g          291 t
ORIGIN      
        1 caatatggag gcgactggtt tttacaggat ttccctgcct tccgcacagc ctggcgatat
       61 cctgctgtgc tgctttggcg catcggtggc caatcatgcc gccatatact gcggcaacgg
      121 tgagctgctt caccatctgc ctgaacaact gagtaaacgg gagaggtatt ccgaaaaatg
      181 gcaacgacga acgcattcag cctggcgtca ccgccactgg cacgtatctg ccttcacggg
      241 gatttacaac gatttggccg ccgcctcagc ctgtatgtga acacggcagc ggaagccatc
      301 cgtgccctgt cgatgcagat gccgggattc cgccgtcaga tgaacgaagg ctggtaccag
      361 atacgtattc gcggtgagga cacggcaccg gaggcggtgt acgcccgtct tcacgaacag
      421 ctgggtgagg gaacggtcat ccatattgtg ccgcgactgg ccggagccgg aaagggcgga
      481 ctgcagattg tgctgggggc ggcagccatc gtgggctctt tcttcactgc cggggcatca
      541 atggcgttat ggggttcagc cctggcagcc ggtggttttt ctgccaccac gatgctgttt
      601 tcacttggag ccagcatgat tctgggcggt gtggcccaga tgctggcccc gaaggcaaaa
      661 acaccggatt accgcgcaac ggataacggc agacagaaca cgtacttttc ctcgctggat
      721 aacatgattg cccaggggaa cccgatgccg gtgccttacg gggaaatgct ggttggctcc
      781 cgccgtatat cccaggacat cagcacccgt gatgaaggcg ggggcggaac ggtcgtggtt
      841 gtcgggcgac agggataaaa cataaaaaaa tcccgcagtg atcgcggagc tgcggggaca
      901 gacaaatgaa gatcaatgtt aaggagttgt ttttgttact cgggcaaaaa aacactaacg
      961 cagcgaaatt ataagcgcca cagtcagtgt gtgaaaatgt gaagatattc agaattttta
     1021 tgccattacc ggttttaacc aacaggatta tcggtgggca tgaaagaaaa ccccggtatc
     1081 tgctgatacc ggggtttctc tttagcatgg cagaaatgtg tttcatgctt ttcgggcgaa
     1141 ggatatccga cttctgtacg gaatggcaag tggcggttaa tttattcagg ggaaggctgt
     1201 atgggaaaag gtggcggtaa ggcacacacg cctcgtgagg cgaaggataa tctcaaatcc
     1261 acgcagatga tgagtgtgat tgatgcgatt ggtgagggac cgatagaagg tccggtgaag
     1321 ggactgcaga gtat
//
LOCUS       DAZGTR010000363         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGTR010000363 DAZGTR010000000
VERSION     DAZGTR010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.367"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..1325
                     /locus_tag="ACHLCY_005325"
                     /pseudo
     CDS             1..1325
                     /locus_tag="ACHLCY_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          305 a          322 c          433 g          265 t
ORIGIN      
        1 ggcctacaag attggcgttg gccacccgcc ttccagtgac aacgtggagg cgctggagca
       61 gcagtattat tcccagtgcc tgcagacgct gattgagtcc attgaactgt tactggatga
      121 ggcgctggaa acgggggaaa acgagagtac ggagtttgac gtcaccacgc tgctgagaat
      181 ggacagcgaa cggcgcatga aaacgctggg tgagtcggtg aaaaatacgc ttctcacgcc
      241 caatgaggcc cgtaaacgtg agaacctgcc gccccttgcc ggcggtgatg cactgtatct
      301 tcagcagcag aactacagtc tggaggcgct gtcccgtcgt gatgcccgtg aagatccgtt
      361 tgcgtcgtcc gggaaaagag tttcagccca actgcctgac ggcgcatctg acggtaataa
      421 ggcaatcagt gaaacagagc atggtgcggt gaaagcgatg ttgaggggga tactgcgaaa
      481 atgacggaac gggaactgtc cattattcgt gcactgggag aagaattcgc cacggtgctg
      541 gcggatttac agcgcacatt tgaggagaag atggccgcgc aggcacaagc gtttgaagag
      601 aaactggctt ccctgtctgt ggtattacgg aagtgcgtga cgggcgatga tatgcgtccg
      661 atgcttgagc agatggtgaa ggaggcggta agccatatcc ctgttccgcg tgacggtcgt
      721 gactacgatc cggaagtact gcagaaggcg gtgaatgatg cggtcgcaaa tattccacag
      781 ccggcggacg gtaaaagtct caccccggat gatgtgcgtc cgatgcttga acagatggtg
      841 aaggaggcgg taagccatat tcctgttccg cgtgatggtc gtgactacga tccggatgtt
      901 ctgcagaagg cggttctgga tgcggtgagt gccctgccgg ctccgcagga cgggcgtgat
      961 gccacggcac tggaaatact ccccgccatt gacgatcaaa aatcctttcc ccggggcacg
     1021 tatgccacac accagggcgg actctggcgg gcgtatgaaa aaacgcacgg gatgcgggga
     1081 tgggaatgcc tggttgacgg ggtggcggat attgacgtca gtatgacggg ggagcggttg
     1141 ttctctgtgg tgatccggca cagcagtggc cagcgtacgg aaaaaacatt ttccctgccg
     1201 gtgatgctct accgcggtgt gttcagagcc ggtgaaacct accaccccgg cgatacggtg
     1261 acgtgggggg ctcgctgtgg cactgcaaca gtatgaccgg tgataaaccc ggagaagctc
     1321 attca
//
LOCUS       DAZGTR010000364         1321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGTR010000364 DAZGTR010000000
VERSION     DAZGTR010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.368"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..882)
                     /locus_tag="ACHLCY_005326"
     CDS             complement(<1..882)
                     /locus_tag="ACHLCY_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP0372332.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNF"
     gene            complement(932..1234)
                     /locus_tag="ACHLCY_005327"
                     /pseudo
     CDS             complement(932..1234)
                     /locus_tag="ACHLCY_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          269 a          436 c          314 g          302 t
ORIGIN      
        1 gaagttatac tgctgattca gggaggccag cgccttcacc ggatctgctg caatcccttc
       61 aaaggctttt tccaccttcc cggcatcgtc ccccaccgtc tgcagccatc tctgagaggt
      121 ttcccccatg atccgtagct gcccggcggt atatttcccg ctttctgcca gacgggccag
      181 attttctgcc gcctgtctga taccaccacc ggcttcatcg ctgatcaccc cggccatttt
      241 ccacaattct gccgttgtgg tggcagccgc ccctccggtc aggatcagtg aacgcaataa
      301 ggcccgatca gcctgctctg cctgccaggc ggcggcagca agcgcggcca gtacggcaac
      361 cccgccacct gccgccacac gggccaccga cataaatcgt cccagctcac cggcattccg
      421 ggcattttca gccagtgcat ttgccgtatc tgacagcgac tcctctgatg attcagaggc
      481 atccctgatc ccgagaagtt cctccttcag cagggtaagc aggctgagcg gtccaccgaa
      541 tgaatcgctg atctgccccc cctgctgcag catgataagg aagggattct gaccaccggc
      601 aagctgagtg acaatatccg tgaactgtgc gggcagtgtg cgcatggcag ccttatactg
      661 tccgactgat atcccggctt tttgtgcagc cagcgcctgt cggctcagac tctgctcagc
      721 agcatcagcc tgttttctga aagactgact gactttcccg gacatcaaat cagcaagcgc
      781 actggtttca cccagctctt tttttacccg cgctgcctct tcagaaaaac gggcagaatc
      841 cagtgtcagc acggctgtca gatcggcaaa attacctgcc atcgtggtca cctcctgaaa
      901 tgtcctctga taccatcaat aactgtcaca acctccttcc ctcctccccg aaacggactc
      961 caccggcgag gcccgccgcc ttctgcatca gtacatcatt ttcgtccggc gtctccgcct
     1021 gtccttcctc tgcctccgga gcgaacaggc tgaaatccgc cggatgcata tccggatcgc
     1081 aaaaaaacag gctgagtacg gcgtacatca gcccggaaaa atgcatatcc agctgggtat
     1141 cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc atattcggtg gatgtcatcc
     1201 cggcaagcat ggcgcgccag tcggctctcc ccatctcacg cgccagtttc aggacaaagt
     1261 tcagctcgcc ttcgaatgct tttttgatgt taccggctca gtcgcttctg ttttcccggt
     1321 t
//
LOCUS       DAZGTR010000365         1319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGTR010000365 DAZGTR010000000
VERSION     DAZGTR010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.369"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..>1319
                     /gene="ygeH"
                     /locus_tag="ACHLCY_005328"
     CDS             24..>1319
                     /gene="ygeH"
                     /locus_tag="ACHLCY_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP0372333.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFPPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHPTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYN"
BASE COUNT          469 a          234 c          224 g          392 t
ORIGIN      
        1 aattttttca tagaggttaa ctaatggact tagaaaataa attctcatat cattttcttg
       61 agggattaac gctcacggaa gatggaattc ttactcaagg aaatgagcaa gtttatattc
      121 cacagaaaga gttaggtgta ttaatagtat tacttgaatc cgctggtcat gttgtactga
      181 aagatatgat catcgaatca gtatggaaaa atattattgt tagtgacgag tccctgacaa
      241 gatgtatcta ttctttgcgc tgcatttttg aaaaaattgg ctatgatcgt tgcatagaaa
      301 caatctaccg gaaaggttat cgtttcagcg ggcaggtttt caaaactaaa attaatgaag
      361 ataatacttc agactattcc atagctatat tccctttcac tacttcgttg aatacactgg
      421 atccattaat acttaatcag gaattagtgc aaatcatttc aaataaaaaa atcgatggtc
      481 tctataccta tccgatggct gcgacaaatt tttgtaatga tcacatatct caaaattcat
      541 tcttgagcag attcaagcca gattatttcg ttacaggaag aataaaccag aataatgcag
      601 tgaacacttt atacattgag ttgatcgacg ctaaaaacct tttcctcatc gccagtaatc
      661 atctccctgt tgatgaacta cataatacat cacaatttat tatagataat atccttcaaa
      721 cggtacataa accagaacga tctgtaagat tagctaagca ggaccaagga tataagaatc
      781 attatttatc agatgaaatg ttagccggaa agaaagaact ttacgacttc ccccctgaaa
      841 gcatttacag ggccatgact atatttgata gattacaaaa taaaagtgat atacagacgc
      901 taaaaacaga atgttattgc cttctagcgg aatgccatat gtctttggca cttcatggaa
      961 aaagtgaact tgaacttgct gctcaaaaag cattagagct tttagattat gtatcagaca
     1021 taaccactgt cgatggaaaa attttagcta ttatgggact gataactggt ctgtctggac
     1081 aagcaaaagt atctcatatc ttatttgaac aggctaagat acacccaact gatatagcct
     1141 ctctctacta ctatagggca ctagtccact ttcataatga aaaaattgaa gaggcaagga
     1201 tttgtataga caaatcacta caactcgaac ccagaagacg aaaagcagtt gtgataaaag
     1261 aatgtgtaga tatgtatgtg cctaacccgc tcaaaaacaa cattaaactc tactataat
//
LOCUS       DAZGTR010000366         1304 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGTR010000366 DAZGTR010000000
VERSION     DAZGTR010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.370"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..818
                     /locus_tag="ACHLCY_005329"
     CDS             <1..818
                     /locus_tag="ACHLCY_005329"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0372334.1"
                     /translation="NVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTG
                     VNLLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSED
                     KLIVFHLMGSHGPTYYLRYPESHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYII
                     SKLIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSP
                     GFVSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(911..1270)
                     /locus_tag="ACHLCY_005330"
     CDS             complement(911..1270)
                     /locus_tag="ACHLCY_005330"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0372335.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          439 a          231 c          232 g          402 t
ORIGIN      
        1 ataatgtgca gtcgtgtggc acatccacag ccatatctgt tccatgcatg ttttcagata
       61 tgaagcgaaa agagttcaat tcccgcaaag cggtaaactc ggaaaatgta ctggatattc
      121 tgtacagaac gggggtaaat ttactctgga ttgaaaatga cgggggatgt aaaggtgtat
      181 gtaagcgaat ccctacaatt aatattgagc catcaaattc agataataca ttatgcaaga
      241 aaaactcatg ttatgatgag gttatgctaa aaaatattga tgagtatata aataataact
      301 cagaggataa acttatcgta tttcatttga tgggtagtca tggtcctacc tactatctcc
      361 gttatcctga gtcacataaa tattttaaac caacatgtga tcgtagtgat atcgaaaatt
      421 gtacgcatga gcaattgatt aatacctatg ataatacaat tcgctatact gattatataa
      481 tatcaaaatt aattgacaag cttatcgaat ataaagatga gtatgatact gttttattat
      541 atgtctctga ccatggagaa tctctgggag aaaatggatt atacttacat ggaacaccat
      601 ataatgttgc tccagctgag caaacgcatg tgcctcttat tacctggatg tctccggggt
      661 tcgtttcctc taaaaaaatt gatttgaatt gtttgtcaga acatgctcta aacagaacag
      721 taagtcatga taatatattt tcatcgttac tggggttatg gaatgtaaat acttcagttt
      781 ataataaaga agatgatatt atatcctcct gcagatagat actgacattt ttctgaggat
      841 aagaggtgaa tacctcttat ccttaagtaa atattaacca ggtaactgtg aataatttag
      901 atgttgggcg ttatgaaagt aataaaactc cccatataat gatagcaact aaaatcgtaa
      961 tcaacactgc ggctgaaccc atatcttttg ctctgcctga taattcgtga tgttctgaac
     1021 caattctgtc aacaactgct tcaattgcac tgttaattat ttcaacaata attaccatca
     1081 ttgccgaacc aatgagtaat actcgagtaa cagagtcaac atccagccaa aaggatatta
     1141 tcagtgctga cagaaccaga atgcactcct gacgaaaggc tgcttcatag cgccatgccg
     1201 cacataagcc tttccatgaa taacccgctg ctttaataat tctggtaaca ccagtggtcg
     1261 tattagccat aaatacctct tatttaatta tatgtctgaa tatg
//
LOCUS       DAZGTR010000367         1298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGTR010000367 DAZGTR010000000
VERSION     DAZGTR010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.371"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..1141)
                     /locus_tag="ACHLCY_005331"
     CDS             complement(<1..1141)
                     /locus_tag="ACHLCY_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0372336.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPDNLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNP"
BASE COUNT          257 a          423 c          363 g          255 t
ORIGIN      
        1 ccgggttaag ttgctccacc acccgccccg catcgtcgta gcggtagcgc atctccggcc
       61 tgcctgcata acggtgcccc accatccggc cccggtattt atcatcgtaa gtaaagtggc
      121 gcatctgcgt accgctgcgg tcatacaccg ccgccagctc accgcgtggc gtccagtcgt
      181 agcacaccag tggcgcggca ggtaaattat ccgggtattc cgggtcgtgc atcagccaca
      241 ccgccgaaag acggataccg ctgtcagcgc cgtattccgt accgggcagt gtgtcgggga
      301 aggaaggagc tgagagaggg cggggactgt caggggaaga taacgaagcg gtgtgcggtt
      361 tacgggcctc ttccgcccgc tgtgcctgtg tggtcagtac cagacggaac tcccgcccgg
      421 caccgtccgt cacgccggtg atttccccgg ccaggtcacc ggcggcctca cgccggtacg
      481 ccagcgtccg cccgaagcgg tccgccatcc cggtaagcac ccggtacggc ggcagcggcg
      541 ctggcagtaa gtcctcagca cccggcaccc gctcagacca ccccagtatc caccacggcc
      601 cctgtgcgct gttggtcgcc aggtaaagat gcgggcttaa ccggatatcc ggtggcagcg
      661 acgcccacag ccgtgccagc gtgtggccgt ccggctgcgc tgccttaccg ccacgcacca
      721 gccacattga ctcactgcgg ctgtacaccg cctcccccgg cagcagcggc tcaaagtgaa
      781 tgctccgccc gccgttgtcg ttgagtatca gtgcatcatc gcgtatctgt aagcggatat
      841 cagaaggcgc tttccagccg gggccgaaaa cgcccaccgg tgcaggcgtc ttcgtccggt
      901 agctgctgta ggtgcgggag agaatgaacg gcagcgggcc gggcagcgca aggtccgtct
      961 cgccgggctg tacttttgca ccgagcagcg ggttcaccgg gttgcccgag gtcatcccgc
     1021 ccggacacac cgagcacgcc acgccagttg gcgcgccaat tcttacacct gccgaaccct
     1081 ggacaatggg accgccatac tgagtcatat ctccctgacg cgccgctggt tttccgctca
     1141 tataaagtgc ccttttagtt attgttcata tccctgtgtt agcaacaaaa aaaataaaat
     1201 gacacacaag accagctaat gtatttatat atttttcaca taaacttatt tgttctcaat
     1261 caacaacaac tgttattaca ggtcactttc aatatttt
//
LOCUS       DAZGTR010000368         1294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGTR010000368 DAZGTR010000000
VERSION     DAZGTR010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.372"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            444..1058
                     /gene="evgA"
                     /locus_tag="ACHLCY_005332"
     CDS             444..1058
                     /gene="evgA"
                     /locus_tag="ACHLCY_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP0372337.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1063..>1294
                     /locus_tag="ACHLCY_005333"
     CDS             1063..>1294
                     /locus_tag="ACHLCY_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFP0372338.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQ"
BASE COUNT          408 a          239 c          257 g          390 t
ORIGIN      
        1 gttttttatt tgaattaatc tgttccacta ttatctctca tttctcatag atgaaattta
       61 tgaattgaat gaaaagagca ttcattcttc atatcaaaca cagtgcatgt gttgcacatt
      121 atcccaggaa cgttggaggc ctaatttaaa tgaggtgcaa aaaaaacaac agaggactaa
      181 accgtggctt ttgcaataca attcttacgc ctgtaggatt agtgagaaga cttatagtgc
      241 taacttgaaa ctataaatca tcggtataat ccctgatttt attgttgaca tttcatttat
      301 gccgactatt tatatggtat acttgtcgaa ttatcttaaa ggaagctcag attttcttat
      361 ttttattgag aaaatgagat gacgccttat gtctgtatta ctacagggag aagggagatg
      421 cttcattgca aagggaataa tctatgaacg caataattat tgatgaccat cctcttgcta
      481 tcgcagcaat tcgtaattta ttgatcaaaa acgatattga aatcttagcg gagttgactg
      541 aagggggaag cgccgttcag cgggtggaaa cacttaagcc tgatatcgtc atcattgatg
      601 tcgatatccc cggagttaac ggtatccagg tgttagaaac gctgaggaag cgccaatata
      661 gcggaattat tattattgtc tccgctaaaa atgaccattt ttacgggaaa cattgtgctg
      721 atgctggcgc taatggattc gtgagtaaaa aagaaggcat gaacaatatc attgcggcta
      781 ttgaagctgc aaaaaatggc tactgctatt tccccttctc tctcaaccgg tttgttggaa
      841 gtttaacgtc cgaccagcaa aaactcgact ccttatcgaa acaagaaatt agtgtcatgc
      901 ggtatattct tgatggcaag gataataatg acattgctga aaaaatgttc atcagcaaca
      961 aaactgtcag cacttataaa agtcgcctga tggaaaaatt agaatgtaaa tcactgatgg
     1021 atctttacac attcgcgcaa cgtaacaaaa tcggctaatc acatgaagtt tttaccctat
     1081 ttttttcttc tctgttgtgg tctttggtcg accataagtt tcgcagacga agattacatc
     1141 gaatatcgtg gcatcagtag taacaaccgt gtcacacttg atccagtacg tctgagcaac
     1201 aaggaattac gttggttagc gagcaaaaaa aatcttgtga ttgcagtaca caagtcccaa
     1261 acggctacct tgttgcatac cgattcgcag caac
//
LOCUS       DAZGTR010000369         1264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGTR010000369 DAZGTR010000000
VERSION     DAZGTR010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.373"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..128)
                     /locus_tag="ACHLCY_005334"
     CDS             complement(<1..128)
                     /locus_tag="ACHLCY_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis family protein"
                     /protein_id="HFP0372339.1"
                     /translation="MVLTVKKILAFGYSKVLPPVIEQFVNPICIFIITPLILNHLG"
     gene            complement(167..>1264)
                     /locus_tag="ACHLCY_005335"
     CDS             complement(167..>1264)
                     /locus_tag="ACHLCY_005335"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFP0372340.1"
                     /translation="DSKMFTMGKKVELYEKNFADLFGSKYAVMVSSGSTANLLMIAAL
                     FFTNKPKLKRGDEIIVPAVSWSTTYYPLQQYGLKVKFVDINKETLNIDIDSLKNAISD
                     KTKAILTVNLLGNPNDFAKINEVINNRDIILLEDNCESMGAVFQNKQAGTFGVMGTFS
                     SFYSHHIATMEGGCVVTDDEELYHVLLCLRAHGWTRNLPKENMVTGTKSDDIFEESFK
                     FVLPGYNVRPLEMSGAIGIEQLKKLPGFISTRRSNAQYFVDKFKDHPFLDIQKEVGES
                     SWFGFSFVIKEGAAIERKSLVNNLISAGIECRPIVTGNFLKNERVLSYFDYSVHDTVA
                     NAEYIDKNGFFVGNHQIPLFNEIDYLRKVLK"
BASE COUNT          405 a          253 c          187 g          419 t
ORIGIN      
        1 ttacccaggt ggttgagtat tagtggtgtg ataatgaaga tgcaaattgg attgacaaac
       61 tgttcaataa ccggtggtag tactttagaa tagccaaacg ctaaaatttt tttcactgtt
      121 aataccatct taaaggcact ctattcgaaa tagagtgcct cgttagttat tttaatactt
      181 ttcgtagata atctatttca ttaaacaaag gtatctggtg gtttccgaca aaaaaaccat
      241 tcttatctat atattcggca tttgctaccg tatcatgtac agagtaatca aaataactca
      301 aaacacgttc atttttgaga aaattcccag taacaattgg tcggcattca atgcctgctg
      361 agatcagatt atttactaaa ctcttcctct caatagcagc tccctccttt ataacgaagg
      421 aaaaaccaaa ccagctactt tcaccaactt ctttttgtat atcaaggaat ggatgatctt
      481 taaatttatc tacaaaatat tgtgcattgg aacgtctggt ggatataaaa cctggtaact
      541 ttttaagttg ctctatccca atagcaccac tcatttcaag tgggcgaaca ttgtatcctg
      601 gtaaaacaaa cttaaacgac tcttcgaaaa tatcatcact cttagtacct gtaaccatat
      661 tctcttttgg taaatttctt gtccaaccat gagctcgaag gcacaacaat acatgataca
      721 gctcttcatc atcagtaact acgcagcccc cttccattgt agctatatga tgagagtaaa
      781 aagaactaaa ggtacccata actccgaatg tgcctgcctg cttattttga aagaccgcgc
      841 ccatcgactc acagttatct tctagtaaga taatatccct attatttatt acctcattta
      901 tttttgcaaa atcattagga ttacctaata aatttactgt caatattgct tttgttttat
      961 ctgaaatagc atttttcaaa ctatcgatat caatatttaa agtttcttta ttgatatcga
     1021 caaacttcac ctttaagcca tactgttgca gagggtaata tgtcgtagac catgacactg
     1081 caggtactat tatttcatca cctcttttaa gttttggttt attagtgaag aaaagggcag
     1141 caatcattaa cagattagct gtagaaccag agctaaccat tacggcatat ttgctaccaa
     1201 acaaatcagc aaaatttttc tcatataact caaccttttt acccatggta aacatttttg
     1261 agtc
//
LOCUS       DAZGTR010000370         1253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGTR010000370 DAZGTR010000000
VERSION     DAZGTR010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.374"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..178
                     /locus_tag="ACHLCY_005336"
                     /pseudo
     CDS             1..178
                     /locus_tag="ACHLCY_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            180..473
                     /locus_tag="ACHLCY_005337"
     CDS             180..473
                     /locus_tag="ACHLCY_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP0372341.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(534..1253)
                     /locus_tag="ACHLCY_005338"
                     /pseudo
     CDS             complement(534..1253)
                     /locus_tag="ACHLCY_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          425 a          225 c          195 g          408 t
ORIGIN      
        1 ttcttactcg ttaaaacaaa tagccattat taaaggaatt gattataaaa caatatcata
       61 tcacaaaata agagccctaa ataaacttaa cataaaaggt actgtttagt tgtttatagc
      121 actctgtgaa tgggataaac attattttaa actccaatct tgcgtaagag aaagttgata
      181 tgtcattagt aggatataat agtaacatca taataaccat cgcgctgata tcgttgctct
      241 ctgcatgcac cagagaaaat tcattaaagg agaatcaaaa taaaagaagt ggcgaatccc
      301 aagcgatagt agatgcaaga cagttcttta gcgaacaccc ggaatatata tcatcagcag
      361 aactggagca aaagctatat cgcgaatttg cacaggttac tacagttcca gcttataaag
      421 agatgagtat gtatcaattg cttatcattt cacaagatcg tttaactcat taaataagga
      481 tctacacgct ttttgcgtgt agattcttat aaatttctga ctacgacaga tgtttaattt
      541 tttaaattat cgtctttatt ctcgattttt gtgtcttcac cctcaatgaa tttatcttct
      601 gaagagagcg gttcatcttg aacaggttgc ccagcattct ccacatcttc aagccacagc
      661 agaagacgta atatttcatc tatattttcg aagctaacat aatcgtagcg acgatgggta
      721 gcataatttt ttcgtgctaa ttttttatct gtgataattg gtattccgat ttcctttgca
      781 tatttcctta cgcctaaggc cacctcatta gttactctta cagaaatcaa tggaataggt
      841 gaaagatgtg gcttaaagta tatccctatt gcaatgtgag tagggttgga aatcatcaaa
      901 cgactattac tgacatcaga cttcaattgc tcagaaagaa tttcctgatg cgctctctgc
      961 gtttagactt aatttctgga tttccttctt gctccttgta ttctctttta acttcttgtt
     1021 tatccatttt catatctttc ataaataaaa aatattcagc gataaaatca aaaattaaga
     1081 caattatcat actgccgaga caatacagta tgagaaggaa tagcatctct ccccaatctg
     1141 ataataaaga tatgatatct ccatcaagag ttttaaaaag cagtgattta tgattactcc
     1201 aaaatacttt gatcgccaat gcaaaaaaaa caatataaag aattgcttta aca
//
LOCUS       DAZGTR010000371         1235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGTR010000371 DAZGTR010000000
VERSION     DAZGTR010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.375"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1007
                     /locus_tag="ACHLCY_005339"
     CDS             <1..1007
                     /locus_tag="ACHLCY_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0372342.1"
                     /translation="QWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPRFKKRGQNDAF
                     RYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVS
                     TPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQLSRKVKFSNNW
                     QKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPG
                     RNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKEN
                     RLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA
                     "
BASE COUNT          341 a          305 c          328 g          261 t
ORIGIN      
        1 cgcaatggct taaagattcg ccctcacagc cattgcaaca gtcactgaaa gaccttgagc
       61 gggcttacaa aaacttcttc cggaagcggg ctgcttttcc ccgattcaaa aagcggggac
      121 agaatgatgc attccgctac ccgcagggcg taaagctcga tcaggaaaac agccgtattt
      181 ttctgccgaa actgggctgg atgcgctacc ggaatagccg tcaggtcacg ggtgttgtga
      241 aaaatgtcac ggtcagccag tcctgcggta agtggtacat cagtattcag acagaaagtg
      301 aagtatcaac tccggttcac ccttcagcat caatgatcgg gctggatgct ggcgtggcta
      361 aactcgccac gctgtcagat ggcacagtct ttgggcctgt aaacagtttt cagaaaaacc
      421 agaagacgct ggcgagactt cagcgacagt taagccgcaa ggtcaaattc agcaataact
      481 ggcagaagca gaaacgcaaa atacagcgac tgcattcccg tatcgcaaat atccgcaggg
      541 actaccttca taaagtcaca acgaccgtca gcaaaaacca cgcaatgata gtcattgagg
      601 atttgaaggt cagcaacatg tcaaagtcag cagcgggtac ggtcagccag ccggggcgca
      661 atgtccgggc aaaatcaggt ttaaaccgtt cgatactgga tcagggctgg tatgaaatgc
      721 gccgccagct tgagtacaag cagctctgga gtggcggtca ggtgcttgct gttccgccag
      781 cgtacacaag ccagcgttgc gcgtgctgtg gtcatacagc gaaagaaaat cgcctgtcac
      841 aaagtcaatt cagatgccag gtatgtggat atacagcgaa cgccgatgta aacggcgctc
      901 gtaacatttt agcggcgggg cacgccgttc ttgcctgtgg agagatggtg cagtcaggcc
      961 gcccgttgaa gcaggaaccc accgaaatga ttcaggcgac agcctgaacg tagcagggat
     1021 cctcgtcatt cagggcgtgg aggatgtcaa gacacggaaa acgaacatga tgtttgtggt
     1081 tggtaattat cactgatcgt tgatggcgca atttctgtgc catccgcaaa aaaatcctcc
     1141 gtgatgctcg tcacaaatct tgccctcccg atgttgtgct atgcttttta tcagcgacta
     1201 acccgctaat gcaaaacaga taagtgagga aacaa
//
LOCUS       DAZGTR010000372         1232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGTR010000372 DAZGTR010000000
VERSION     DAZGTR010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.376"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            457..894
                     /locus_tag="ACHLCY_005340"
     CDS             457..894
                     /locus_tag="ACHLCY_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUKH-3 domain-containing protein"
                     /protein_id="HFP0372343.1"
                     /translation="MCELINEASKYGKVNERKMDIDLCCKKIEEEGYSVFVAFYDFIK
                     KYGGLYGFHSAYKDTTINEAFFIDPERASNRIFIERVNDYIERAGYNMIPVGECSNGH
                     ITLLYGENNVIYGGLDDYLWKYGDNLSSSIDILINGKEAVEIP"
     gene            914..1232
                     /locus_tag="ACHLCY_005341"
                     /pseudo
     CDS             914..1232
                     /locus_tag="ACHLCY_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
BASE COUNT          374 a          220 c          298 g          340 t
ORIGIN      
        1 catttgatta tataaaagac atgattgtaa atgtttcaaa aactgaaaga ttggatggtt
       61 attttcgtgt gaatgatgat gaagataatt tattcttaga atggattgta aaagatggtt
      121 atttagagga aataattttg tctggttcat aaattcatct tctttgttga gaagattaac
      181 tgcctaggtg aaattatcct ctatgaccgg gacgctgcag gccacctggc agaagtcatc
      241 acgcaggaag acaagtcgct gtatcccttt gaaaaatccg gcaggctaat gggcatcttc
      301 agcccaggcg gcacatcaca gcgaatttga aggcaaatgc gaaaattagt gatcttgaag
      361 ttcatacagt attggtaaaa acaggcgaag cttttccaat gtgtaagaat tttcaaattt
      421 caatatttgg cgctgttgtt acaagcgggt gattatatgt gtgaattaat taatgaagca
      481 tcaaaatatg ggaaagtcaa tgaaagaaag atggacattg atttgtgctg taaaaaaata
      541 gaggaagaag gatattctgt tttcgttgcg ttctatgatt ttataaaaaa atatggcggt
      601 ttatatggct ttcattcagc ttacaaagac accactataa atgaggcttt ttttatagat
      661 ccagaaaggg cctctaatcg catttttata gaaagagtca atgattatat tgagagagct
      721 ggttacaata tgattcctgt tggcgagtgc agtaatggtc atatcacatt attatatggg
      781 gaaaataatg tgatatatgg cggcttggat gattatcttt ggaagtatgg ggataattta
      841 tcttcatcaa ttgatatatt gattaatggc aaggaggcag ttgaaattcc gtaaggccaa
      901 cttgatatga cacctggcac acaaccccga cgaccggatg tatatgctgg gcgggatgag
      961 tgacaccgcc agcaaccgca tcacgtttga gcgcgacagc cagtaccgga tcacgtgtgt
     1021 cagtcacacc gacgggatcc ggcttaaact gacgtaccat gccagcggct acctgaaagc
     1081 cattcaccgc acggataacg gcatacagac gctggcgacc tacgaacagg atgcgcgggg
     1141 gcggctgacc gaagcggatg cgcggctgga ctaccacctg ttttatgagt acgacgctgc
     1201 ggaccggatc atccgctggt ccgataacga cc
//
LOCUS       DAZGTR010000373         1217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGTR010000373 DAZGTR010000000
VERSION     DAZGTR010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.377"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..588
                     /locus_tag="ACHLCY_005342"
     CDS             <1..588
                     /locus_tag="ACHLCY_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0372344.1"
                     /translation="YITQDPIGLKGGWNLYGYQLNPISDIDPLGLSMWEDAKSGACTN
                     GLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPN
                     RGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSF
                     YLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            560..943
                     /gene="yhhH"
                     /locus_tag="ACHLCY_005343"
     CDS             560..943
                     /gene="yhhH"
                     /locus_tag="ACHLCY_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFP0372345.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1048..>1217
                     /locus_tag="ACHLCY_005344"
     CDS             1048..>1217
                     /locus_tag="ACHLCY_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFP0372346.1"
                     /translation="MSNTYQKRKASKEYGLYNKCKKLNDDELFRLLDDRNSLKRISSA
                     RVLQLRGGQDAV"
BASE COUNT          440 a          174 c          273 g          330 t
ORIGIN      
        1 tatatcactc aggatccgat tggactgaag gggggatgga acctgtatgg atatcaattg
       61 aatccgatat cagacatcga ccccctgggt ttatctatgt gggaggatgc aaaatcgggg
      121 gcatgtacta atggtctttg cggcacacta tccgctatga taggtccaga taaatttgat
      181 tctatagata gcaccgcata tgacgcctta aataaaataa atagccaatc tatttgcgaa
      241 gataaagagt tcgctggttt aatatgtaag gataatagtg gcagatattt ctcaacagca
      301 cctaaccgag gagaaagaaa aggatcatat ccattcaata gcccttgccc taatggtact
      361 gagaaagtat cagcttatca tactcatggt gcagatagtc atggagaata ttgggacgaa
      421 atattttcag gtaaagatga gaaaatagtt aaaagtaaag ataacaatat caagtcattt
      481 tatttaggta cgcccagtgg taattttaaa gcaatagata accacgggaa ggaaataaca
      541 aacagaaaag gattacctaa tgtctgcaga gttcatggta atatgtaaaa aaatattgtt
      601 taggaactgt gtcattgtat ctttgtttgt ttttacatac aacacatggg cgcagtgtaa
      661 taataatatt aaaataatgc gcaagtatga aagtgaaggt aaatataccg ttagaaattt
      721 ggttaaaaat aaagctatag cattggaatt agctgagata tatgttaaga atcgttatgg
      781 acaggatgcc gcagaagaag aaaaaccata cgaaatcact gagttaacaa caagttgggt
      841 tgttgaaggt accattcact cagaccaaat tgctggtggg gtttttatta tagaaatagg
      901 caaaaatgat gggagaattc tgaattttgg ccacggaaaa taagtatgta aggataatca
      961 aacatcgtgt gcgctgatgg cagagtaggt ggaggactcc agacagtcaa acgatagaaa
     1021 aagatagcct ttatggaggt tcctgcaatg tcaaatacat accagaaaag aaaggcaagt
     1081 aaagagtatg gtttatataa taaatgtaag aaactaaatg atgatgaatt atttcgctta
     1141 cttgatgatc gcaattcctt gaaaaggatt tcatctgcca gagtattaca gttaagaggg
     1201 gggcaagacg ctgttag
//
LOCUS       DAZGTR010000374         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGTR010000374 DAZGTR010000000
VERSION     DAZGTR010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.378"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1212
                     /locus_tag="ACHLCY_005345"
     CDS             <1..>1212
                     /locus_tag="ACHLCY_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0372347.1"
                     /translation="RLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKTLSEIQKQELLRNA
                     ALIDQQKIREQLRSREETLKNENAAARASNEAELLGYGQGERARERMRELQQIRDSFR
                     QKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQEAFYAESDVQRADWQKGLQE
                     GFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWAASILQEISKVLM
                     NAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPT
                     YFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDG
                     TNGQIGPEAAG"
BASE COUNT          312 a          272 c          410 g          218 t
ORIGIN      
        1 ccgactgggg tatgcggcgg atgacctcgg ttttgtgggt gatccgtatc aggaggcgag
       61 acaacgttat atcagtaatg ccctggaagc ctggcgcaat aacgaggcga ataaacccaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctgctgaa
      181 gcagcagaaa gagcaactgg cactggccgg tcagaacacg gagctggcga agctgaagta
      241 ccagacagcg ctgggtgaac tgaaaaccct gtcggagata cagaagcagg agctgctgcg
      301 taacgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 cctgaagaat gagaatgcgg ctgcgcgtgc atcaaatgaa gccgaactgc tggggtacgg
      421 gcagggagaa cgagccaggg aacgcatgcg ggagttgcag cagatccgcg acagcttccg
      481 ccagaaggat gcggaccttc agtctcagta tcagaccggg gatatcagtg aggattttta
      541 cagacaggcg ctggcgcaga atgcacagta tctgagtgaa cgtctgaaag agcaggaagc
      601 cttttatgcc gaatcggatg tgcagcgtgc ggactggcag aaagggctgc aggagggatt
      661 cagtaactgg gtggataatg cgtccgatta cgcctcacag gcagcacagc ttgcgacgga
      721 gggtatctca gggatggtga ataacatcac ggagatgctg aacggaaata aagtggaatg
      781 gcgcagctgg gctgcatcaa tcctgcagga aatatcaaaa gttcttatga atgccgcgat
      841 tgtcaacgga attaagacgg cggcaaacgg tatgtccggt gcgggaggat ttctcggcag
      901 cattggtgac tggctgggcg gggcggtggc caatgcaaaa ggcggcgtgt atacctcggc
      961 aaacctgagt gcgtacagca acagtattgt ggatacgccc acgtactttg cctttgcaaa
     1021 aggggccggg ctgatggggg aggccggacc tgaagccatt atgcctctga cgcgggcggc
     1081 ggatggctcg ctgggtgtgc gagcggtggg cagtatgaac ggtagtgcgg gtctggtgta
     1141 ttccccggtc taccatatcg ccattcagaa tgacgggact aatggccaga tagggccgga
     1201 agctgcgggc ag
//
LOCUS       DAZGTR010000375         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGTR010000375 DAZGTR010000000
VERSION     DAZGTR010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.379"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1212
                     /locus_tag="ACHLCY_005346"
     CDS             <1..>1212
                     /locus_tag="ACHLCY_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0372348.1"
                     /translation="RLGYAADDLGFVGDSYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKAQLALAGQNTELAKLKYQTAQGELKTLTEIQKQELLRNA
                     ALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARERMRELQQIRDSFR
                     QKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQETFYAESDAQRADWQKGLQE
                     GFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRSWASSVLQEISKVLM
                     NAAIVNGIKTAANGMSGAGGFLGSIGDWLGGAVANAKGGVYTSANLSAYSNSIVDTPT
                     YFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDG
                     TNGQIGPEAAG"
BASE COUNT          307 a          277 c          411 g          217 t
ORIGIN      
        1 ccggctgggg tatgcggcgg atgaccttgg ttttgtgggg gattcgtatc aggaggcgag
       61 acagcgttat atcagtaatg ccctggaagc ctggcgtaat aacgaggcga acaaacctaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctgctgaa
      181 gcagcagaag gcgcaactgg cactggccgg tcagaataca gagctggcga agctgaagta
      241 ccagactgcg cagggcgaac tgaaaaccct gacggagata cagaagcagg agctgctgcg
      301 caatgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 actgaagaat gagaatgcgg ctgcgcgtgc gtcgaatgat gctgaactgc tggggtacgg
      421 gcagggggag cgagccagag aacgcatgcg ggagttgcag cagatccgcg acagcttccg
      481 ccagaaggat gcggaccttc agtctcagta tcagaccggg gatatcagtg aggattttta
      541 cagacaggct ctggcacaga acgcgcagta tctgagtgaa cgccttaaag accaggaaac
      601 tttttatgcc gaatcggatg cgcagcgtgc ggactggcag aaagggctgc aggagggatt
      661 cagtaactgg gtggataatg cgtccgatta cgcctcacag gcagcacagc ttgcgacgga
      721 gggtatctca gggatggtga ataacatcac ggagatgctg aacggaaaca aagtggaatg
      781 gcgcagctgg gcctcatccg tactgcagga aatatcaaaa gttcttatga atgcggccat
      841 tgtcaacgga attaagacgg cggcaaacgg tatgtccggt gcgggaggat ttctcggcag
      901 cattggtgac tggctgggcg gagcggtggc caatgcaaaa ggcggcgtgt atacctcggc
      961 aaacctgagt gcgtacagca acagtattgt ggatacgccc acgtactttg cctttgcaaa
     1021 aggggccggg ctgatggggg aggccggacc tgaagccatt atgcccctga cccgggcggc
     1081 ggatggctcg ctgggtgtgc gcgcggtggg cagtatgaac ggcagtgcgg gtctggtgta
     1141 ttccccggtc taccatatcg ccattcagaa tgacgggact aatggccaga tagggccgga
     1201 ggcggcaggc ag
//
LOCUS       DAZGTR010000376         1207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGTR010000376 DAZGTR010000000
VERSION     DAZGTR010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.380"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            707..1176
                     /locus_tag="ACHLCY_005347"
                     /pseudo
     CDS             707..1176
                     /locus_tag="ACHLCY_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535762.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          357 a          229 c          252 g          369 t
ORIGIN      
        1 tgtcttttac aaaaaataaa gattattgac aaaaccaaat ataaataacg actgcctcgg
       61 catgccagtt aacagctatc cgtatggcgt gttcggtttt gcgaataaaa aatggttact
      121 gtagaagcgg atttccacca catgtcaacc atgaatggtc agtgatgcga gacgactggg
      181 atgatttggt cgatgacttc ttccggcgaa tactgataga aacattgaaa tattgtaaga
      241 gaaagcgttc aaaattctgt ggcacgtgaa aaattctaca gcgtcatcac cttattcagt
      301 cgaccttcaa aataaacgtt taacagagac aatcaccatt tggattgtcc gctgaggagg
      361 cgaacaattt cctagacagc tttatcatgt cagcccacat tgttggcgct ggcaatacta
      421 aggacaaacg atggggaagt agctttttag cgttattttc gggagtaaaa aacgtggtat
      481 ctttccgtat gtcagttgta tcttgctggt attcgtagtc gtgataaggc atacaacgat
      541 atttatcttg acgttagtga agaataagtt gttttgtgat ctatgttaat ctgggattac
      601 aatccgggct tgatacatcg catcacgact tttattctct tcagaataga atatgttact
      661 ccccgcttta caggctcagg atttgaatta caaattatac ttttaagtga acaataacca
      721 agggtatttc ttatttcctg aaaccaggct tggcccattt cgggatgagt ttgaaaaaac
      781 tcttgacgca tacgatagtg atgaaataaa cgaaacaaat tacgtcaaca aactcaggcg
      841 tcttgcctga catgctcctg acatcattga tattcacgct catctggcat atgcatttgc
      901 gctctttatg ctgcaattct ggcgaatgtt catttacagc gtcatcagga tgccgttata
      961 ctcattgata aaatactaac atataaccct gagaataatc agggaacacg ctggttactg
     1021 ggggcggaat tgttacgtgc tggcaagcat ggcagggctc tgtacgtatt gacaggacat
     1081 gtcaatgaat ttccccccta ctggtacgag cttgctcttt tacattttat gaatgaggca
     1141 tatattaatg ctgcaacggc atttcgatat ggatttttaa caagtatata ttgttgaaat
     1201 gctttgt
//
LOCUS       DAZGTR010000377         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGTR010000377 DAZGTR010000000
VERSION     DAZGTR010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.381"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..314)
                     /locus_tag="ACHLCY_005348"
     CDS             complement(<1..314)
                     /locus_tag="ACHLCY_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372349.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDIPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NT"
     gene            complement(336..875)
                     /gene="yhaB"
                     /locus_tag="ACHLCY_005349"
     CDS             complement(336..875)
                     /gene="yhaB"
                     /locus_tag="ACHLCY_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP0372350.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPLVHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1131..>1196)
                     /locus_tag="ACHLCY_005350"
     CDS             complement(1131..>1196)
                     /locus_tag="ACHLCY_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFP0372351.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          365 a          214 c          226 g          391 t
ORIGIN      
        1 caggtattgt ccgatcgttg aataatttgg gcgttataac ctaaaaaatc cagacgaaga
       61 gacgagtcat tataaagaat tacattttgt aagtacgcta attctttttg aaaatcacca
      121 gtgataatgc attttccacc aggttgtaac gtgttaagta aagtgaccat ttcaatagag
      181 gtctgtttta gtttttctat acctggtgct agtgcttcga ggttatttcc tgttgggctt
      241 tcaggaatgt cattcatttt tcgacggact aaatcactac taatatcatt accaattgat
      301 gaaacaggga acattatttt atcctttata aataatcaat aaaaatcatt attaaaatga
      361 tcgggtgaaa aaaagagaat ttgccaacaa tctgaagtaa tgctggtttt attaaagtgc
      421 acaaggggtg ttaagatggc ttcttttcgg gtgaattttg cacaatactt agaaatcgtc
      481 tccttaatac attccagctc ttttttattg acggctgagc aagtacattg ttcgccaaaa
      541 ggtgcaaaag tgcctacatc taatgtttgt atgatataat catcatgttc ccctgtagaa
      601 atatctatga tgaatggact catattttcc ggttggattc ggatcattac agaaggaaca
      661 tcgccttgat agaaattcaa tgcagtaatt gcagataata ctctccgcag ttcattactt
      721 atactgtaaa ttttattaac gtcattggtg gagacaattt ctgcaaactt tctttttacc
      781 gtccaataat caatatttct attgtgattg ttaaccactg cgtgcattgg cgggattgaa
      841 gggtgaaaaa tatgtgcaat tgggaagcct ttcattgatg cctgccttgt cgtcatgaca
      901 aacaataata gatgcttatt ttcctcgcag ggttctttcg cactatcaaa aaacgctcat
      961 ttacccggcg ttacagtaaa cgaagcaaca ttctgtggcc tacgacgatc aaaagctctg
     1021 tgaaaatttc ccttgttgtc tttgtctgac cttatcgcgg cgaccagcaa ttattcgttt
     1081 tgtgatcctc aacacaaaat gctattcgtc gcctatgagg ggagaagaat ttatggaaaa
     1141 cgattgcatt cacaaaatga cgtcctgtca gaagagaaaa agttgacgtt aatcaa
//
LOCUS       DAZGTR010000378         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGTR010000378 DAZGTR010000000
VERSION     DAZGTR010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.382"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..557
                     /locus_tag="ACHLCY_005351"
     CDS             <1..557
                     /locus_tag="ACHLCY_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0372352.1"
                     /translation="PMLEQMVKEAVSHIHVPRDGRDYDPDVLQKAVLDAVSALPAPQD
                     GRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSM
                     TGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMT
                     EDKPGEAHSSAWTLAAKRGRDAGG"
     gene            560..886
                     /locus_tag="ACHLCY_005352"
     CDS             560..886
                     /locus_tag="ACHLCY_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0372353.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            896..>1196
                     /locus_tag="ACHLCY_005353"
     CDS             896..>1196
                     /locus_tag="ACHLCY_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0372354.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPD"
BASE COUNT          256 a          287 c          413 g          240 t
ORIGIN      
        1 gtccgatgct tgaacagatg gtgaaggagg cggtaagcca tattcatgtt ccgcgtgatg
       61 gtcgtgacta cgatccggat gttctgcaga aggcggttct ggatgcggtg agtgccctgc
      121 cggctccgca ggacgggcgt gatgccacgg ctctggaaat actccccgcc attgacgatc
      181 aaaaatcctt tccccggggc acgtatgcca cacaccaggg cggactctgg cgggcgtatg
      241 aaaaaacgca cgggatgcgg ggatgggaat gcctggttga cggggtggcg gatattgacg
      301 tcagcatgac gggtgagcgg ttgttctctg tggtggtccg gcagagcagt ggccagcgta
      361 cggaaaaaac attttccctg ccggtgatgc tctaccgcgg tgtgttcaga gccggtgaaa
      421 cctaccaccc cggcgatacg gtgacgtggg ggggctcgct gtggcactgc aacagtatga
      481 ccgaagataa acccggagaa gctcattcat cagcctggac cctggctgca aaacgtgggc
      541 gggatgcagg aggctgaaaa tgacggcatt actgacactg gaagagatca aggcacatct
      601 gcgtgtcgac catgacgcgg atgatgacat gctgatggac aaggttcgtc aggctaccgc
      661 cgtgctgctg gcctacattc agggcagccg ggataaagtg atccgtgagg acggtgaact
      721 gatcccgggc gaggcattaa cccggatgaa gggggctgcc atgcgactga ccgggatgct
      781 gtaccggaat ccggatcttg cggagcggga agaactgctt cagggggagc tgccgttttc
      841 tgtttccgtg ctgatttacg atttgcgttg tccgacggtg ttatgaggag ggggaatggc
      901 aatatctgca ggtcgtctga cacagatgat aagtgttctg aacccggtgt taacccgtaa
      961 cgctgccgga gaaatgacgg aagaatgggt gtcatgcggg aaaattcatg cggatatccg
     1021 aggcaggagc agccgggagc ggatgcagtc cggtgcggaa atggcgcagg cggaaatccg
     1081 catctgggtg cgcggtcagt ccggtcggga aatcacggca gcgtcacgac ttcatgtgct
     1141 gagtggtcca tggcgtgacc ggatcctgaa cgttgtcggg ctgcccgtgc cggatg
//
LOCUS       DAZGTR010000379         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGTR010000379 DAZGTR010000000
VERSION     DAZGTR010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.383"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1196
                     /locus_tag="ACHLCY_005354"
     CDS             <1..>1196
                     /locus_tag="ACHLCY_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0372355.1"
                     /translation="SIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPP
                     AVQHLTVEVSAADGQYLAQAKWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETT
                     YRFTQLAPGSYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITAVPKL
                     AVYDPTVQFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVN
                     LVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADEMAEMKTSI
                     TETRNEITQTVNKTLENQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDGIPYVAGIG
                     AGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLKRLFAVSIT
                     SSGNPPT"
BASE COUNT          296 a          305 c          376 g          219 t
ORIGIN      
        1 tccatccggg aaaacacgga cggtaccttt gccatcacgg cggtgcagca cgtaccggaa
       61 aaggaagcca tcgtggataa cggggccagc tttgagccgc agtcaggcac cctgaacagc
      121 gtcatccctc cggcagtgca gcacctgacg gtggaggtga gcgcagctga cggtcagtat
      181 ctggcacagg cgaaatggga cacgccgaag gtggtgaagg gcgtgagctt tatgcttcgc
      241 ctgaccgtgg ccgcggatga cggcagtgag cggctggtca gcacggcccg gacgacggaa
      301 accacatacc gcttcacgca actggcgccg gggagctaca ggctgacagt ccgggcggta
      361 aatgcgtggg ggcagcaggg cgatccggcg tcggtatcgt tccggattgc cgcaccggca
      421 gcgccgtcac agattgagct gacaccgggc tattttcaga taacggcggt cccgaaactg
      481 gctgtatatg acccgacggt acagtttgaa ttctggtttt cggaggcaaa aatcgcagac
      541 acatctcagg tggaaacctc tgcccgttat ctggggaccg gcagtcagtg gagtgtatcc
      601 ggcccgcaca ttaagcccgg gaaggatttc tggttttacg tgcgcagcgt caacctggtg
      661 gggaaatctg cgtttgtgga agtcagcggg cagcccagca atgatggtga agggtatctg
      721 gaatttttcc gggaaaaaat aggaaaactg catctggctc aggggttgtg ggaactgata
      781 gataacagcc agcttgcaga tgagatggcg gagatgaaga ccagcatcac ggaaacccgc
      841 aatgaaatca cacagacggt caacaaaaca ctggagaacc agagcgccac catacagcag
      901 atacagcgcg tgcagaagga cacaaatgat gacctggctg cgctgtacat gctgaaggtt
      961 caaaaaacga aagacggcat tccctatgtg gccgggattg gtgcagggat tgaggatact
     1021 gatggccagc cactgagcaa catactgctg ctggctgacc gtatcgcgat gataaatccg
     1081 gagagcggca acagcacgcc gttatttgtg gcgcagggga atcagctgtt catgaacgac
     1141 gtgttcctga aacgactgtt tgcggtgagt atcacgtcat ccggcaaccc cccgac
//
LOCUS       DAZGTR010000380         1182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGTR010000380 DAZGTR010000000
VERSION     DAZGTR010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.384"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1182
                     /gene="rhsD"
                     /locus_tag="ACHLCY_005355"
     CDS             <1..>1182
                     /gene="rhsD"
                     /locus_tag="ACHLCY_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFP0372356.1"
                     /translation="GLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRRE
                     YDEPGRLVSETSRSGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCS
                     GYQTRYEYDRFGQMTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTA
                     VITPDGNRSETQYDAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALD
                     RLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQY
                     DDHGWLTDISHLSEGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQ
                     GLANRVTPDSLPPVEWLTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNA
                     AY"
BASE COUNT          275 a          335 c          397 g          175 t
ORIGIN      
        1 ggtctgaatg tggtgtcagg cgatatcacg gacatcacca cgccggacgg gcgggagacg
       61 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
      121 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
      181 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
      241 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
      301 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
      361 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
      421 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
      481 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
      541 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
      601 agtctgacta acgagaacgg cagccacagc gacttcagct acgatgcgct ggaccggttg
      661 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
      721 ctcacgcaga gtgaagatga ggggcttgtc accctctggt actacgatga atcggaccgt
      781 atcactcacc gcacggtgaa cggcgaaccg gcagagcagt ggcagtatga tgaccacggc
      841 tggctgacag acatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
      901 gacgataagg gccgcctgac cggcgaacgg cagacggtgg agaacccgga gacgggggaa
      961 ctgctgtggc agcatgagac aacacacgcc tacaacgaac aggggctggc aaaccgcgtc
     1021 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta tctggcgggc
     1081 atgaagcttg gcgacacgcc gctgctggag tacacgcgcg accgcatgca ccgggagacg
     1141 gtgcgcagct ttggcagcat ggcaggcagt aatgccgcat at
//
LOCUS       DAZGTR010000381         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGTR010000381 DAZGTR010000000
VERSION     DAZGTR010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.385"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..570)
                     /locus_tag="ACHLCY_005356"
     CDS             complement(<1..570)
                     /locus_tag="ACHLCY_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0372357.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLYIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSAD"
     gene            839..955
                     /locus_tag="ACHLCY_005357"
                     /pseudo
     CDS             839..955
                     /locus_tag="ACHLCY_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1060..1136)
                     /locus_tag="ACHLCY_005358"
     tRNA            complement(1060..1136)
                     /locus_tag="ACHLCY_005358"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1100..1102),aa:Arg,seq:tct)
BASE COUNT          279 a          332 c          307 g          262 t
ORIGIN      
        1 gtccgcactg aatgtcccct cagcgccctg ggtgaatgca gaaccaccac gacagcatgg
       61 taccagcagg atccccgcgt tattcgggat atacggaagc agttttttgg caatatataa
      121 gccctggccg acacagccgt actgcccttt gctcaggtct gccttcggat gattcagcgt
      181 actcatatcc tgcacatcat gcaggcagtg gtcagccgga atgatgtcgt tatacgtaca
      241 actctctcca cccggagtta ccgtgctgcg gcgcgccagc tgtttaatgc gcggatccgg
      301 agcatcgtaa gaatccggta acggaagccc ttcaccgtag gccatgccgt tggactgtcc
      361 ggcaagcaca accacgtaga accagtccgg ctcagatgaa gggccgacct gtggatctcc
      421 ttcaatagcc accgcctgca tcagtgtgta cggcgtaatg gcaaccggtc cgccgtatgg
      481 ctgccagccc tctttcagtt tgtgtgtcag cttttccgca aggtctgacg gcgacgccgc
      541 cctgacaaca tcatagtgtt taaatgccat gaatcctccc ggccgggata atattgtgag
      601 taaaatgagg agcgggctga agtccggaag ttacaggaca atggcagaag agagacaaca
      661 gcccgcaata cgaaaaaggc cgcgctattg cgcagagtga ttactgtcgg atattattcg
      721 ccagctgaaa tattacttca cgttttgttg tttattcctt gccgcccgcg tctccctgcg
      781 cgggcttttt ttgtccataa gaaagcccct ccggagaggg gctggagagt ggcgctatgt
      841 gccattgcat ggtgccgggt gcctcccggt gaattcagta ccagcacctg aatccgcgat
      901 tatcccatat acctactcgc tgattgcccc tccgcacagg gggattcacc atgccagttt
      961 cttttaacaa actccccgca aaccagacaa cagtcaaccg cctgaattgt gagacattta
     1021 aaaaaaaggc ccgcaaaagc gagccaggga aaataagtgt ggcgcgttgt actggattcg
     1081 aaccagtgac cgattgctta gaaggcaatt gctctgtccg gctgagctaa caacgcagga
     1141 tacagataat ggaccgcctt cggggacccg aactccgcgc
//
LOCUS       DAZGTR010000382         1155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGTR010000382 DAZGTR010000000
VERSION     DAZGTR010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.386"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..928
                     /locus_tag="ACHLCY_005359"
     CDS             <1..928
                     /locus_tag="ACHLCY_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0372358.1"
                     /translation="ADEIKVDRSDYYAKTISVVDSIDNDSQIENVFNLLDSLYVESVD
                     TLTFKYQSIIKGLDRLFEGINLDSAFSLSEEERSYFEEKAKNLNALAQLGISVEIISH
                     ELEEMDSMVTRGLNSLPTSVKEHPGFSLALNAHRSLTQQIRFLSPLKISGYQSRQRIT
                     GKNIMDYVLKFFGERFERQRITIEFSEEFKQIAITDIPSRIYPVFTNIINNAMYWVSL
                     SNNRLIKIGFVNSLVIIANSGPAIDTDDIPRLFELFYSKRANGHGVGLYLCRENLAVA
                     HHKIWYSEPDEGDNYLIKDGANFVIQFNGVEF"
     gene            930..>1155
                     /locus_tag="ACHLCY_005360"
     CDS             930..>1155
                     /locus_tag="ACHLCY_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFP0372359.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNG"
BASE COUNT          385 a          180 c          217 g          373 t
ORIGIN      
        1 ggccgatgaa ataaaggttg atcgaagtga ttattatgct aaaactatat cagttgttga
       61 ttcaatagat aatgattcac aaattgaaaa tgtgtttaat ttgctcgata gtttatatgt
      121 tgagtcagtt gataccctaa ctttcaaata tcaatcaata ataaaaggtc tcgatagatt
      181 atttgaaggt ataaacttag attcagcatt ctcattatct gaagaagaac gctcatattt
      241 tgaagaaaaa gctaaaaatt taaacgcgct tgcacagtta ggtattagtg ttgagataat
      301 atctcatgaa cttgaagaaa tggattctat ggtaaccaga ggactaaact ctcttcctac
      361 ttctgtaaaa gaacaccctg gtttttcatt ggcgttaaat gctcacagat cgcttactca
      421 acaaatacgt ttcttatcac ctttgaaaat atcaggttat caatccaggc agagaataac
      481 tggaaaaaat atcatggatt atgtcctgaa gttctttggg gagcgtttcg aacggcaacg
      541 aataactatt gaatttagtg aagagtttaa gcaaatcgca ataacagata taccatcaag
      601 gatctatcct gtttttacta atattatcaa caatgcaatg tattgggtca gtctgtcaaa
      661 taataggctc ataaagattg gttttgtgaa ttctttggtt atcatagcaa attctggtcc
      721 ggcaattgat accgatgata tcccgcgact atttgaacta ttttatagca aaagagcaaa
      781 tggacatggg gtaggtctgt atctatgtcg agaaaacctt gctgttgcac atcataaaat
      841 atggtattca gaacctgatg aaggcgataa ctatttaata aaagatggcg ctaattttgt
      901 gatccagttc aatggagtgg agttctaata tgacagtggc aaattataat tctcttgtcc
      961 agaaaacttt ctgcgaaaat gcaattcgtt ccgttgtcat gattgatgac gattttctga
     1021 cgtattctga atcaatcagg gcgttgaata acgaagttga tttagactac aacaaaattg
     1081 actcatctaa acgagccgct actcttgaga gcttttttca atctaaaaat atgatttgtg
     1141 atgttgacaa tggtt
//
LOCUS       DAZGTR010000383         1133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGTR010000383 DAZGTR010000000
VERSION     DAZGTR010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.387"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            63..872
                     /locus_tag="ACHLCY_005361"
     CDS             63..872
                     /locus_tag="ACHLCY_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0372360.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            865..>1133
                     /gene="yqeJ"
                     /locus_tag="ACHLCY_005362"
     CDS             865..>1133
                     /gene="yqeJ"
                     /locus_tag="ACHLCY_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFP0372361.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYT"
BASE COUNT          387 a          211 c          212 g          323 t
ORIGIN      
        1 ataccaatat acttagcaaa ctatgtgatc tccatttcga ttgatttagt gtttattgac
       61 gtatgtactg gattattaac gataatatcg agttctggcc tgagcaccga aaattaatat
      121 cggtacataa cgccgatctt aacgtcgttc tgacaacgcc agccagtcga tgtttatcac
      181 ttctacttga agcttttcct gatgtggttg cacaacaaga ttttttcacc agagtctggg
      241 aagaagaagg tatgcgtgtg cctactaaca cgttatatca gaacatatcc attatcagac
      301 gcggatttcg cgctgttggt gatactaccc actcgctaat tgcaaccgtg ccgagaagag
      361 gattcaagat ccataatgac attaacatac aaaatcatgt aataaactcg tcaacagacg
      421 cacatacaca caatgcccca cctgccataa aagttaatgc gggatacaaa gagagcattg
      481 gtggcgcaaa gaatttcaat aacaaaatcc tcaaacatat aaaaagtcat ctaattatgt
      541 tgagcgcatt tgtcataggc gcatattctg catattggct atggaataat aatcaaccga
      601 agccattttt caaggattac aagactgttg cggaaattaa tggctgccat tttaatgtaa
      661 cagaagatac aattgacggg ttgaaagagt tcgataaata taaaacacgg atactggatt
      721 cggggattaa ctgtaaaaaa catccgtggt tatacttccc tcttgcaaaa tcctcaccgg
      781 ggatgattgt tatggcatgc aataaaaatt ataaccaaca tgaagtggca gactgtttaa
      841 ccctgtctta ccgtgaggtt aatcgtgatt gattataaaa aaaacttatt atttatttta
      901 gtttttatca gtgggtttat tctcttcact gtctattctt acactgcaga aaaaatgata
      961 tataacgaaa catgcactgc aaattgggta atttttaatg accagggacg agcaaatctt
     1021 accatcgact ttatgtataa caaaaaaaat aaaacaggaa ctgttgcgtt gagtggaacc
     1081 tggcaacaag gtaacagaga aagtaaatca atacggcgaa atattgaata cac
//
LOCUS       DAZGTR010000384         1132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGTR010000384 DAZGTR010000000
VERSION     DAZGTR010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.388"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..579
                     /locus_tag="ACHLCY_005363"
     CDS             <1..579
                     /locus_tag="ACHLCY_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0372362.1"
                     /translation="VARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAA
                     FGQFVTANLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQY
                     VGDQLVLVNAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSV
                     AIRAERWINWRRRRTAAVAVITGVNYGSASGG"
     gene            624..845
                     /locus_tag="ACHLCY_005364"
     CDS             624..845
                     /locus_tag="ACHLCY_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372363.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            791..>1132
                     /locus_tag="ACHLCY_005365"
     CDS             791..>1132
                     /locus_tag="ACHLCY_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0372364.1"
                     /translation="MWNLLRRTRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAK"
BASE COUNT          230 a          298 c          376 g          228 t
ORIGIN      
        1 gtggcgcgtc tggatacaga ctttgtggac ccgaaaaaag ccgcggtggc ggatgtctcc
       61 ccggcgtcca tcacccatga tgtgaagggc acggcatcaa ccggtaaccc ggatgcggat
      121 gccgaggccg cgtttggcca gtttgtgacg gcaaatctgc agcccactgg tgctgtctgg
      181 ctgatgtcca gcaccaatgc cctggcgctg tccatgcgta aaaatgcgct gggtcagaag
      241 gaatacccgg acatgaccct gcttggcggc accttccagg gcctgccggt gattgtctcc
      301 cagtacgtgg gtgaccagct ggtgctggtg aatgcgccgg atatttatct ggctgatgac
      361 ggcggtgtgg cggtggatat gtcccgtgaa gcgtcactgg agatgcagtc tgaaccgacc
      421 ggcgacagta ccacgccgtc cccggtggag ctggtttcca tgttccagac aggcagcgtg
      481 gccatccgtg cggagcgctg gatcaactgg cgtcgtcgcc gtactgcggc ggtggcggtg
      541 atcaccggag tgaactacgg cagtgcgtcc ggcggctgag tctgatgagg aggacgggag
      601 gcgtgcgcct cccgtaacag gttatggcaa agatccgata tctgcagggc acgcatgatg
      661 cccgggccgg ggatatccgt gatgtggcac agccgtgtgc ggaggtgctg gttcgcctgg
      721 ggaaggcgga gtacatcaca gtgcgacgtc cggcaggtca gaaaaagaaa cgtgatgcgg
      781 agcatggcga atgtggaacc ttttgcggcg aacccgaaaa aaccagaaat caggacgtga
      841 tgtaaaagag gtgggctgga ccagcctgtt tcaggcggtg gctgagcctt ttgccggcgc
      901 ctggcagcag ggcgtgaaag ccgatccgga aagtgtcctc tcctttcatg cggtgttttc
      961 ttgcatttcg ctgatatccc aggatatcgc caaaatgcga ctgcgcctga tgcagaccga
     1021 tacacagggg atccgccgtg aaaaacggca gggggatatt gcccgtctct gtcgtcgtcc
     1081 caatgcacag cagaatcgta tccagttttt tgaactgtgg ctgaacgcca aa
//
LOCUS       DAZGTR010000385         1129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGTR010000385 DAZGTR010000000
VERSION     DAZGTR010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.389"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..1098
                     /gene="gmd"
                     /locus_tag="ACHLCY_005366"
     CDS             <1..1098
                     /gene="gmd"
                     /locus_tag="ACHLCY_005366"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0372365.1"
                     /translation="TGITGQDGSYLAELLLEKGYEVHGIKRRASSFNTERVDHIYQDS
                     HLANPKLFLHYGDLTDTSNLTRILKEVQPDEVYNLGAMSHVAVSFESPEYTADVDAIG
                     TLRLLEAIRILGLEKKTKFYQASTSELYGLVQEIPQKETTPFYPRSPYAVAKLYAYWI
                     TVNYRESYGMFACNGILFNHESPRRGETFVTRKITRGIANIAQGLEKCLYLGNMDSLR
                     DWGHAKDYVKMQWMMLQQETPEDFVIATGIQYSVREFVTMAAEQVGIELAFEGEGVNE
                     KGVVVSVNGTDAKAVNPGDVIISVDPRYFRPAEVETLLGDPTNAHKKLGWSPEITLRE
                     MVKEMVSSDLAIAKKNVLLKANNIATNIPQE"
BASE COUNT          355 a          192 c          250 g          332 t
ORIGIN      
        1 actggtatca ctgggcaaga tggctcctat ttggcagaat tattgttaga aaaaggttat
       61 gaagttcatg gtattaaacg ccgtgcatct tcatttaata ctgagcgagt ggatcacatc
      121 tatcaggatt cacatttagc taatcctaaa ctttttctac actatggcga tttgacagat
      181 acttccaatc tgacccgtat tttaaaagaa gttcaaccag atgaagttta caatttgggg
      241 gcgatgagcc atgtagcggt atcatttgag tcaccagaat acactgctga tgttgatgcg
      301 ataggaacat tgcgtcttct tgaagctatc aggatattgg ggctggaaaa aaagacaaaa
      361 ttttatcagg cttcaacttc agagctttat ggtttggttc aagaaattcc acaaaaagag
      421 actacgccat tttatccacg ttcgccttat gctgttgcaa aattatatgc ctattggatc
      481 actgttaatt atcgtgagtc ttatggtatg tttgcctgca atggtattct ctttaaccac
      541 gaatcacctc gccgtggcga gacctttgtt actcgtaaaa taacacgcgg gatagcaaat
      601 attgctcaag gtcttgagaa atgcttatac ttgggaaata tggattctct gcgtgattgg
      661 ggacatgcta aggattatgt caaaatgcaa tggatgatgc tgcagcaaga aactccagaa
      721 gattttgtaa ttgctacagg aattcaatat tctgtccgtg agtttgtcac aatggcggca
      781 gagcaagtag gcatagagtt agcatttgaa ggtgagggag taaatgaaaa aggtgttgtt
      841 gtttcggtca atggcactga tgctaaagct gtaaacccgg gcgatgtaat tatatctgta
      901 gatccaaggt attttaggcc tgcagaagtt gaaaccttgc ttggcgatcc tactaatgcg
      961 cataaaaaat taggatggag ccctgaaatt acattgcgtg aaatggtaaa agaaatggtt
     1021 tccagcgatt tagcaatagc gaaaaagaac gtcttgctga aagctaataa cattgccact
     1081 aatattccgc aagaataaaa aagataatac attaaataat taaaaatgg
//
LOCUS       DAZGTR010000386         1122 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGTR010000386 DAZGTR010000000
VERSION     DAZGTR010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.390"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(336..1025)
                     /locus_tag="ACHLCY_005367"
     CDS             complement(336..1025)
                     /locus_tag="ACHLCY_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0372366.1"
                     /translation="MNNQYLQFVREQLMIATADLSGATKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFATSSWRRTLLELEEHQK
                     AWLLWCYGGNICWEHQIAITQWVWNEFKTQSGTRKIAVKTLERVKKLIWLAAQDVRGW
                     VTGCEVYQRQELARLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKA
                     TFSRRDIAKVN"
     gene            complement(1022..>1122)
                     /locus_tag="ACHLCY_005368"
     CDS             complement(1022..>1122)
                     /locus_tag="ACHLCY_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372367.1"
                     /translation="RFNEQFDEINIVRGKPVSGGRLEIKITEVGCA"
BASE COUNT          284 a          313 c          216 g          309 t
ORIGIN      
        1 catatccctc cggcagaagc agaaaaagct tattatgctt ccatcggaaa cgatgatctg
       61 gcagcctgag ttcacagata aaacactctc caggaaaccc ggggcggttc acatcgcgtt
      121 tctcaaactt tcctttttcc ggttaacgtg acacaccaat aactcttgtc gaaaaagcca
      181 gcaagctgaa agaccggtat tcaccgccgc cagcgcgttt actgtactga cgcgattttc
      241 ggacataaaa aaaacgcctg gcggcagttt ttttcttact ttgccatcgc gtacaaaata
      301 ggcaaaatat cagatttata cgaaacatac gcgatttaat tgacttttgc aatatctcgt
      361 cgtgaaaagg tcgctttttg ttgcgctctt attttcacgg agcaaatcaa ggattctcta
      421 tcgaggcgct taaaaatatc gcacatctca cgccagtagt tcgcataatt atggctccag
      481 ttatcaggct taactccaca cagtctggca agctcctgtc tctggtagac ctcacacccg
      541 gtaacccatc ctctgacatc ctgtgccgcc agccagatca acttcttcac acgctccagc
      601 gttttcactg caatttttct ggtaccagac tgagttttaa attcattcca cacccactgc
      661 gttatcgcga tctgatgctc ccagcaaatg tttccgccat aacaccacaa caaccacgcc
      721 ttctgatgtt cttccagttc cagaagggta cgccgccacg atgatgttgc aaactcaaca
      781 ggactgacca gggcaattga tgagcctttc gccagtgatt gtttacccgg gattggggga
      841 ttatcccgcg tgatcatttt tccggttacc tcatcgcggt aacgaatttt tttgcgtctg
      901 taacgccctg tatcgaacag ggcattttcc tgccaggctt ccagttgacc ttttgtcgcc
      961 ccactgagat ccgcagtggc aatcatgagt tgctcacgaa caaactgtaa atactggtta
     1021 ttcatgcaca ccccacctct gtaattttta tctccagccg tccaccagat actggcttgc
     1081 cacgtacaat attgatttca tcaaactgct cattgaaccg cc
//
LOCUS       DAZGTR010000387         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGTR010000387 DAZGTR010000000
VERSION     DAZGTR010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.391"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1112
                     /locus_tag="ACHLCY_005369"
     CDS             <1..>1112
                     /locus_tag="ACHLCY_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP0372368.1"
                     /translation="DYPYFFEKLKKQIAAGRFELQGCFWVECDLNLVSGESLIRQIMH
                     GTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQKLSQNKINKFNNYLF
                     RWQGLDGSEILMHNFPEDTYDSRARARSLEYIEQNYNEKEICPYALMVYGVGDGGAGP
                     GEEHIERLTRIRNIDGLPHVDFSRVDKFFTHADAFRESLPIISGELYFEAHQGCFTSE
                     SATKAHNRIMENKLHDAEFFITITNNMTSILRSEFDEIWKAMLTLQFHDILPGSCISR
                     VYHETEKEYLKLEAKTEKIISDAQSTLLSKIDTSSYKDPHILFNTTCFARNEWININN
                     NWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNY"
BASE COUNT          356 a          222 c          211 g          323 t
ORIGIN      
        1 gattatccct atttcttcga aaagttaaaa aaacaaatcg ccgcaggccg ctttgagtta
       61 caaggttgct tttgggtcga atgcgattta aatctggtaa gtggtgaatc gctgataagg
      121 caaatcatgc atgggacacg attcacactg cagaattttt caaagaagat aaattatgtc
      181 tggcaaccgg atgtctttgg attccccgcc acgcttccac aaattctcaa aaaatcaggc
      241 attaactata ttgcatcgca aaagctctca caaaacaaga ttaataaatt taataattac
      301 cttttccgct ggcaaggcct ggatggctca gaaattctga tgcataactt tcctgaagat
      361 acgtatgata gccgagcccg cgctcgctct ttagaatata ttgaacaaaa ctataatgaa
      421 aaagaaatct gtccctatgc gcttatggtg tatggtgtcg gtgatggcgg cgctggcccc
      481 ggtgaggaac atatagaacg attaacacgc atacgcaata ttgatggctt acctcatgtt
      541 gatttcagcc gtgttgataa gttcttcact cacgccgacg cctttcgcga atccttgccc
      601 attatttcgg gtgaacttta cttcgaggct catcaagggt gctttacatc agagtcagca
      661 actaaagccc ataatcgaat tatggaaaat aaactccatg atgctgaatt ttttataact
      721 attactaata atatgacgtc aattcttcgc agtgagtttg atgaaatatg gaaagcgatg
      781 ctgaccttgc aattccatga catccttccc ggctcatgta tttcaagagt ttatcatgaa
      841 actgaaaagg aatatcttaa actagaagca aaaaccgaga aaattatttc tgatgcgcag
      901 tctactttat taagtaaaat tgacacttca tcttataaag atccgcacat tttatttaat
      961 accacctgtt ttgctcgtaa cgaatggatc aatataaaca acaactggct caaagcacga
     1021 gtaaactctt atggttatgc tgtgattgat ccaaagaata agatcgtaaa tggtctgaag
     1081 gcagaatctc gaagtattga aaataattac at
//
LOCUS       DAZGTR010000388         1107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGTR010000388 DAZGTR010000000
VERSION     DAZGTR010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.392"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..106)
                     /gene="gpU"
                     /locus_tag="ACHLCY_005370"
     CDS             complement(<1..106)
                     /gene="gpU"
                     /locus_tag="ACHLCY_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP0372369.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQ"
     gene            complement(119..742)
                     /locus_tag="ACHLCY_005371"
     CDS             complement(119..742)
                     /locus_tag="ACHLCY_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372370.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(745..1026)
                     /locus_tag="ACHLCY_005372"
     CDS             complement(745..1026)
                     /locus_tag="ACHLCY_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP0372371.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1019..>1107)
                     /locus_tag="ACHLCY_005373"
     CDS             complement(1019..>1107)
                     /locus_tag="ACHLCY_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0372372.1"
                     /translation="AADGAVAAGVAWENAPANSATVAVKINV"
BASE COUNT          220 a          361 c          272 g          254 t
ORIGIN      
        1 cctgttcatc aataaaaacc gggcgaccgt caaaccagct caccctctca gaaatatttt
       61 ctttcagggc agacagaact gccgcccgta tttcacggtg tttcatacac cctcccagtc
      121 attttctttt cagcaccagg cgtaactgat gcgtcatggc tttcatcatc tgcgccggta
      181 atttttcccg gtacatccgg tcccgttcac gttcaaaggt ttctgccagc ggtccggcag
      241 tcgtaatctt caccacttcg atcggcagac ggtggcgttt cggcctccct ttgctgtccg
      301 cgccggtgga tgatgatgcc cacggcatac gctgcatcac atgccagcgt ccgttagcca
      361 gccgggtgat aaaggcgtcc gggatccgtc ttttccccac aatcagcaca ctgccgcccc
      421 ctttcagggc cgcacgctgt cctttctttc tccgttttct gcgggaaagt cgaacgcggg
      481 cctcccccag tttgatggcg ggcaggttgc cggtattgat gtaaaccttt gcgtaaacct
      541 tatccggtcg tgccggactt aaccggatgc gggcacggat aagacgacgg ggaacggcca
      601 gctccctggc aactgaagag gccgttttcg caataatggc ccccgccact cggttcagtg
      661 tcgtggcaga ggcccgggga acggcacggc gatcaattgc atccagattt ttcatggcct
      721 gcgccagacc ttttattgcc atactcattc ctgttcgaca aaaatccggg gtttaccgtt
      781 gtacgtgtca taacgggtca ccgtcagtgt gcgccccgca aacacaacaa catcatgacg
      841 ggccggacgg taccgggctg aaaacaccac cagtgacaac tggctgcccg aaagcgcccc
      901 catctccgcg gactcttcct ccggcatcac gtcgtacacg acgccgttaa tctccgcctg
      961 tttgcccatc acccgaacgg tcgccacgtc catccggcaa cacattcgcg taaacagatc
     1021 agacattgat ttttaccgcc acagtggcgc tgtttgcagg ggcattttcc caggctaccc
     1081 ccgctgccac cgcaccgtct gcagcca
//
LOCUS       DAZGTR010000389         1106 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGTR010000389 DAZGTR010000000
VERSION     DAZGTR010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.393"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..836)
                     /locus_tag="ACHLCY_005374"
     CDS             complement(<1..836)
                     /locus_tag="ACHLCY_005374"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP0372373.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VAS"
     gene            complement(900..>1106)
                     /locus_tag="ACHLCY_005375"
     CDS             complement(900..>1106)
                     /locus_tag="ACHLCY_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0372374.1"
                     /translation="GVLVHGGQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMA
                     LFNAVSLMSLNPEPKKKAYEVFFI"
BASE COUNT          225 a          350 c          289 g          242 t
ORIGIN      
        1 gaagccaccg cggccacatt cccgttaccg gcctgtttca ccggctgcgc cgtggcggcc
       61 ttaccggctt ccagttcacg caggcgcttc aggtgcgcat ccacctgacg gatttccgct
      121 gcggtgttgt cgtaatgctc ttcctcctcc acatccagcg tgcgcccttc ctctgcggct
      181 ttggtcatga cctcctcaag ggaggctgcc agcgctgcac gcttgttttc aaaactttta
      241 atctgttcgc caatattcat tatggtcttt tccttatgaa aaacggttgt tgactgtgcc
      301 gcagcgccgg cagaagatgc gattttcacc accggtttcc ggttgccgga cgcggcagaa
      361 aatgggcggt cgtaagattt aatggtccgg atggtgcatt ccgcattcgc gggcacggtg
      421 acggcagaca cctccatcag ttcccagcgc agaaaatgca gtccgcctcc gtccagaaag
      481 gtgtattcat ggggacggaa gcccacggac agccccctga ccagcccggt cttaatggcc
      541 gcccagacct catccagccg ggctgccatc tgggagggca tccccggctc cggcttcacc
      601 agcattgccg tgatttccag cccttccctg acccgacgca ccgtacactg gcctacaggg
      661 cgggaatggt catgctgcca gagaaacggg atcgtactgc caaactccgc cccctccggc
      721 tccaggatgt caccatcccg atccggagaa ggcgttgacg caatcccggt gatcacccgt
      781 tcatcctcac tgaaggattt caccgtcagc agggaacagg cccgtttaag agtcacatca
      841 gcctcctgaa aataaaaaaa ccgccgcagc ggttcatgat ggttacaggg tgagcagggt
      901 tatatgaaaa aaacctcata cgctttcttt ttcggttccg gattcaggga catcagggag
      961 accgcattga agagcgccat cagcgggtca atttttcccc gtccgctggc ctgtttggtg
     1021 ataagaatgg cgttaccttt aggctccacc cgggcattgc caacgcacca ggccatcagt
     1081 ggctgaccac catgcaccag cactcc
//
LOCUS       DAZGTR010000390         1105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGTR010000390 DAZGTR010000000
VERSION     DAZGTR010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.394"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..104)
                     /locus_tag="ACHLCY_005376"
     CDS             complement(<1..104)
                     /locus_tag="ACHLCY_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0372375.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDE"
     gene            complement(117..740)
                     /locus_tag="ACHLCY_005377"
     CDS             complement(117..740)
                     /locus_tag="ACHLCY_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372376.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     TEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(743..1024)
                     /locus_tag="ACHLCY_005378"
     CDS             complement(743..1024)
                     /locus_tag="ACHLCY_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP0372377.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFEGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1017..>1105)
                     /locus_tag="ACHLCY_005379"
     CDS             complement(1017..>1105)
                     /locus_tag="ACHLCY_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0372378.1"
                     /translation="AADGAVAAGVAWENAPANSATVAVKINV"
BASE COUNT          220 a          361 c          274 g          250 t
ORIGIN      
        1 tgttcatcaa taaaaaccgg gcgaccgtca aaccagctca ccctctcaga aatattttct
       61 ttcagggcag acagaactgc cgcccgtatt tcacggtgtt tcatacaccc tcccagtcat
      121 tttcttttca gcaccagacg taactgatgc gtcatggctt tcatcatctg cgccggtaat
      181 ttttcccggt acatccggtc ccgttcacgt tcaaaggttt ctgccagcgg tccggcagtc
      241 ggaatcttca ccacttcagt cggcagacgg tggcgtttcg gcctcccttt gctgtcagcg
      301 ccggtggacg atgatgccca cggcatacgc tgcatcacat gccagcgtcc gttagccagc
      361 cgggtgataa aggcatccgg gatccgtctt ttccccacaa tcagcacact gccaccgcct
      421 ttcgtgaccg aacgctcgcc tttctttcgt cgctttctgc gggaaagccg cacgctggcc
      481 gtccccagtt ttatggcggg cagattaccg gtgttgatga tgaccttcgc ataaaccctg
      541 tctgcgctgg cccgttgcag gcggatacgc tcacggatga gacggcgcgg aaccgccagc
      601 tccctggcaa ccgaagaggc cgttttcgcg atgatggact ccgccacacg gttaagtgtc
      661 gtggcggcag cccggggaac ggcacggcgg tcaattgcat ccagattttt catggcctgc
      721 gccagacctt ttattgccat actcattcct gttcgacaaa aatccggggt ttaccgttgt
      781 acgtgtcata acgggtcacc gtcagtgtgc ggccctcaaa cacaacaaca tcatgacggg
      841 ccggacggta ccgggctgaa aacaccacca gtgacaactg gctgcccgaa agcgccccca
      901 tctccgcgga ctcttcctcc ggcatcacgt cgtacacgac gccgttaatc tccgcctgtt
      961 tgcccatcat ccgaacggtc gccacgtcca tccggcaaca cattcgcgta aacagatcag
     1021 acattgattt ttaccgccac agtggcgctg tttgcagggg cattttccca ggctaccccc
     1081 gcggccaccg caccgtcggc agcca
//
LOCUS       DAZGTR010000391         1090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGTR010000391 DAZGTR010000000
VERSION     DAZGTR010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.395"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..80)
                     /locus_tag="ACHLCY_005380"
     CDS             complement(<1..80)
                     /locus_tag="ACHLCY_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372379.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFG"
     gene            complement(77..>1090)
                     /locus_tag="ACHLCY_005381"
     CDS             complement(77..>1090)
                     /locus_tag="ACHLCY_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0372380.1"
                     /translation="TKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQ
                     QAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPAALAKATSALKNT
                     WSAEEQLRGSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGAEAD
                     WRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASSGTAIEAAAANFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKGAQDELRLQLRDGGMLSG
                     SGR"
BASE COUNT          205 a          398 c          249 g          238 t
ORIGIN      
        1 tcaccaaaac gcacttcacg caccgacggc tgcgagttca cctccatatc cggcttcact
       61 ttccagcgaa aggttttcat cgcccgctcc ccgataacat accgccatca cgcaactgca
      121 gccggagctc atcctgcgcc cccttgcggg ccatgtcata caccgccttc atcagctgcg
      181 gccccgcctg tccgttggtg ccgtcgttct gaatcaccac gtgattgttc tgattaaaat
      241 taataccttc cgcccgccgc atctgcgccg gacttccggc accacccaca taaccacctt
      301 ccgcatagcc gcgcatcaga cggtaaagat tccccacacc tatccggctg gttgcctctt
      361 tcgtgaaaac aaactccccg cggtgaacta tccccgcagg ctcatatttg ccgcccgtcc
      421 ccgtaaatcc tccggtcgcg aaatggaagt tcgccgccgc agcctcaata gccgtccccg
      481 aggaagcaga tgcaccacca ccgaaagcac cgccaatggc gctgccgata cgcccgacaa
      541 tgcccaccat ggcctgttta agcaggattt ctgtcatcat ggacagcacc gaacgggtga
      601 atccccgcca gtctgcctct gcaccggtca gcatcgccgc catattctgt gcaataccgt
      661 caaaggtctg cgtggccacg cttttaacct gcgaaaaact gtccgtcgca ctttccgccc
      721 actcgcccca gccggacttc agcccggcca tccagcttcc acgaagctgc tcctccgcag
      781 accaggtgtt cttcagtgcc gatgtggcct tcgccagcgc agccggatta tcaccgtaca
      841 cgtcccgaag acgctgcgct tcagactccc gctgcgcctg acggtcagtg agaccgcggg
      901 cttttgcgct gatggcggcc tgcttcgcgc tctgctgctc ttcaaaccgc accgcctgct
      961 gtgccagctc attcaggcgt ttctggtatt caaccttgtc gcccagctca gccagctggc
     1021 gtttgtactc cagcgtctcg tctttatgcg ccagcaggga tttttcctgc tcagataact
     1081 gtcgtttcgt
//
LOCUS       DAZGTR010000392         1089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGTR010000392 DAZGTR010000000
VERSION     DAZGTR010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.396"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..186
                     /locus_tag="ACHLCY_005382"
     CDS             <1..186
                     /locus_tag="ACHLCY_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372381.1"
                     /translation="NFREELVRWYRYRSSSSLPLDELYEHSPAARRYPRDRVLRRLFK
                     LNNEFQRNRIIRSLDFK"
     gene            197..433
                     /locus_tag="ACHLCY_005383"
     CDS             197..433
                     /locus_tag="ACHLCY_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFP0372382.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RVAWAPSVAAATEKWNRRAGDEANLSASQRSNQK"
     gene            390..836
                     /locus_tag="ACHLCY_005384"
     CDS             390..836
                     /locus_tag="ACHLCY_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP0372383.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGKAA"
     gene            833..>1089
                     /locus_tag="ACHLCY_005385"
     CDS             833..>1089
                     /locus_tag="ACHLCY_005385"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0372384.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQC"
BASE COUNT          287 a          255 c          307 g          240 t
ORIGIN      
        1 aattttcgtg aagagttggt gcgttggtat cggtatcgtt catcgtccag tttgccgctt
       61 gatgagttgt atgagcattc acctgccgca cgacgctatc cgcgtgaccg tgttcttcga
      121 cggttgttca aactcaacaa tgagtttcag cgcaacagaa ttatccggag tctggatttt
      181 aagtgaagga gtgagcatga gcgacctatc attaacccag ccaaagctaa aagaatgtcc
      241 gttttgcggc ggtaatgctc gtctgtgggt tgaggccgga ataaatattg atgtgtgggg
      301 ctatgcagaa tgtgacctct gtgaagccag ggtggcatgg gcaccatcag ttgctgcggc
      361 tactgaaaaa tggaaccgga gagcaggaga tgaagcaaac ctttctgctt cgcaacgaag
      421 caatcagaaa taacgccata gacgccattc tctcactacc catcgacgac aagtcacccc
      481 acgaagtcca cgttaaagaa cccaagcgca gcaaagcgca gaatgaccgc atgtggccga
      541 tgctgaacga tgtttcgcgt caggtgctat ggcatggtca acggctggcg ccggaagact
      601 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa agaagtgcgc
      661 ctggtatcga cggtggcgtt gtcatgcttg gcgtgcgtac cagcaaaatg cgaaaggcca
      721 gcatgactga gcttatcgaa atcatgttct ggttcggctc agagcgcaac gtgcggtgga
      781 gtgatgactc ccggcgagag tatgaatggt cacaacgaaa agggaaggct gcatgactat
      841 caaatcaaat acgccagcac acgacaagga ctgctggcaa acgccgctct ggctttttga
      901 tgcgctggat attgagtttg gattctggct ggattcagct gcgagcgaca aaaacgctct
      961 gtgcgctcac tggctaactg aggctgacga cgcgctaaat tctgagtgga taagccacgg
     1021 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
     1081 gcagtgcat
//
LOCUS       DAZGTR010000393         1088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGTR010000393 DAZGTR010000000
VERSION     DAZGTR010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.397"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..122
                     /locus_tag="ACHLCY_005386"
     CDS             48..122
                     /locus_tag="ACHLCY_005386"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372385.1"
                     /translation="MKSSHFTAQVEGAYRLIRNPSVSP"
     gene            297..>1088
                     /locus_tag="ACHLCY_005387"
     CDS             297..>1088
                     /locus_tag="ACHLCY_005387"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0372386.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRT"
BASE COUNT          278 a          287 c          319 g          204 t
ORIGIN      
        1 ttgtgtcact gacctcttct ctcgctcagc atgcaggtct ttctattgtg aaatcatccc
       61 acttcactgc tcaggttgaa ggtgcgtacc gcctgatacg taacccgtcg gtgtccccgt
      121 aagccattgc cgaagccgga tttactgcca ctgcaagggc atgtgaggca catccgctgc
      181 tcctcgcgct ggaagatacc accaccatta acttcggtca cagtacggca tttgatgacc
      241 tggggaatac caccactaac ccgaagacgc acgggctgct ggctcactct gtcctgatgt
      301 acgcccccga cagcgccctg ccagtcgatc ttatcgaaca gcagtgctgg agtcgtgcca
      361 cggacaccta cggtgttaag caccagcgaa aagaacgtcc ctgtgaagaa aaagaaagtt
      421 accgctggca gcaggcttcg gagcgcatgg ccgaacggct gggggagata cagaaacagg
      481 taatcactgt ctgtgaccgg gaagcggata tctggcatta cctgcattat aaagtcagcc
      541 gcgggaagcc acacgctgaa tgtgatgcaa aaaggcgggc gggcagcccg ccaggcccgg
      601 atgctcatca gttacagcga agtcagcata aaaaatcccg acaacagcgg ccaggcgctc
      661 tcgctcacgt atgtctgttg cggggagcag gagaggacgg agcctgctgg tatccgctga
      721 catcagaaaa agtggcgagt gccgccgatg cgcgacgtat cgtcagccat tacgagcaac
      781 gctggctgat agaggaatac cacaaagcgt ggaaaagtgg tggtacatgt gtggaatcgc
      841 tgcgaatgca gacccgggat aaccttgagc gcatggtggt tatcgcggtg cgggtgctgg
      901 gcctgcggca gggaggtatc agtgaagaaa cacaaaatga cagctgtgag aaaatactga
      961 cgccgacaga gtggaaacta ctgtgggtta agctggaagg aaagcagctt ccggcgcaaa
     1021 caccgaccct gaaatgggcc tgtttgaaac tggctaaact ggggcgatgg catgacagca
     1081 aacgcaca
//
LOCUS       DAZGTR010000394         1080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGTR010000394 DAZGTR010000000
VERSION     DAZGTR010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.398"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1080
                     /locus_tag="ACHLCY_005388"
     CDS             <1..>1080
                     /locus_tag="ACHLCY_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP0372387.1"
                     /translation="VAVATAIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKP
                     GIPRPALDSWDAEYMQENRWDEASASFVPVETESDPMNVTFDKLAPEVQNAVMVKFDT
                     CENITVDMVISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKP
                     GAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARARANQQNTENSTGKISQVIAAT
                     HREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWAKNEVIDNDREDWKRISASMRK
                     QPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVFEDEGRNQSATDTLS
                     SPVPETDAVETAIPDNEKTECKVEVE"
BASE COUNT          314 a          266 c          275 g          225 t
ORIGIN      
        1 gtcgctgtag ccactgccat tgaagccggt gatgaacgtc gcgcccgcgc aaaatttcac
       61 tggcaattcc tggaacatta tccggctgct caggactgcg cttataaatt tcttgtttgc
      121 gaggataaac ccggtatacc ccgccctgcc ctcgattcct gggatgctga atatatgcag
      181 gaaaaccgct gggatgaggc gtctgcttcc tttgtcccgg tcgagactga atcagatccg
      241 atgaacgtca cttttgacaa gctggcccct gaagtacaga acgctgtcat ggttaagttc
      301 gacacatgtg aaaacatcac cgttgatatg gtgattagcg cgcaggaatt gttgcaggaa
      361 gacatggcaa cattcgacgg acatatcgtt gaagcgttga tgaaaatgcc agaagttaac
      421 gccatgtatc cggagcttaa gctgcacgcc atcgggtggg ttaagcataa atgtaagcct
      481 ggtgccaaat ggcccgaaat tcaggcagag atgcgcatct ggaaaaaacg tcgcgaaggt
      541 gaacgcaagg aaaccggaaa atacacgtct gttgttgatc tcgcccgcgc cagagccaat
      601 caacagaaca ctgaaaattc aacaggaaaa atcagccagg tcattgctgc cactcatcgc
      661 gaatacaagc agacatggaa aacactggat gacgaactgg cctacgctct ctggcctggt
      721 gatgtggatg ccggaaacat tgacggcagc atccatcgct gggcgaaaaa tgaagttatc
      781 gacaacgacc gcgaagactg gaagcgtatc tcggcatcaa tgcgcaaaca gcctgatgcc
      841 cttcgctacg accgccagac tatttttggc cttgtccgtg aacgtccgat cgacattcac
      901 aaagaccctg tggcactgaa caaatacatt actgaatacc tgactacaaa gggcgtgttt
      961 gaagatgaag gaagaaatca gagcgcaact gatactctct cgtcgccagt accagaaact
     1021 gatgcagtgg aaacggcgat tccggacaac gaaaaaaccg aatgcaaagt ggaagtcgaa
//
LOCUS       DAZGTR010000395         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGTR010000395 DAZGTR010000000
VERSION     DAZGTR010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.399"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..987
                     /locus_tag="ACHLCY_005389"
     CDS             <1..987
                     /locus_tag="ACHLCY_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0372388.1"
                     /translation="TRGGSYGVDPQIHNNDKAKALKSATEHTLQAVARKWLDEKVKTS
                     GISQDHAADIWRSLERNVFPGLGNVPINEIRPKLLKQHLDPIEQRGVLETLRRIISRL
                     NEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPALPPSELPRFMESLTNASIRLETR
                     MLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPADFMKMKKLHKVPLSKEALRILEL
                     MKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMGFGGELVAHGMRSIARTAAEESGK
                     FRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLMQWWSDYVQAQRSNALVA"
BASE COUNT          336 a          227 c          247 g          267 t
ORIGIN      
        1 acccggggcg gttcatatgg tgttgatccc caaatccata acaacgataa ggcgaaggca
       61 ttaaagagtg ctactgagca cactctccaa gccgtagcgc ggaaatggtt agatgagaag
      121 gtaaagacat caggtatctc acaagaccat gcagcagaca tctggcgcag cttagagaga
      181 aatgtctttc ccggtctggg taatgtccct atcaatgaga tccgacctaa gctcttaaaa
      241 caacaccttg atcctattga gcaacgaggc gtattggaaa ctctacgccg tatcatttca
      301 cgtctgaatg aaatcttccg gtgggcagct actgaagaac ttattgagtt caacccggct
      361 gacaaccttg gtcaaagatt cagtaaacca aaaaagcaaa atatgcctgc ccttccccca
      421 agcgaattgc caaggtttat ggaatctttg acgaatgcgt caatccggtt ggaaacacgt
      481 atgctaattg aatggcaatt gttgacatgg gttcgtccgg gtgaagccgt tcgcgcaagg
      541 tggtctgata ttgatacaac caacagcatt tggaacattc ctgctgattt catgaaaatg
      601 aaaaagcttc acaaagttcc tttgagtaaa gaagctttgc gcatccttga attaatgaaa
      661 tcaataagtg ggcatagaga atgggttttc cccagcataa aagcgcctct taatcatatg
      721 catgaacaaa cagccaacgc agctatcatc cgaatggggt tcggaggcga gcttgtagct
      781 cacggtatgc gttctattgc acgaacagcg gcagaggagt ctggtaaatt cagagctgaa
      841 gttcttgagg cagcgcttgc ccactcgaaa aaagatgaaa ttatcgcagc atacaatcgt
      901 gcagaatatc tgatagagcg acagagtttg atgcaatggt ggagtgatta cgttcaagct
      961 caaagatcaa atgctctggt agcctaagta tcagaatagc taatataatc ctgaaggtaa
     1021 agaaaatgga aaccctattc aaagtttttg aaaaatttag ttccagacca ctttttt
//
LOCUS       DAZGTR010000396         1069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGTR010000396 DAZGTR010000000
VERSION     DAZGTR010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.400"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..122)
                     /locus_tag="ACHLCY_005390"
     CDS             complement(<1..122)
                     /locus_tag="ACHLCY_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0372389.1"
                     /translation="MFPATKGALMRIYRRKCKCCNEWFIPKYQNQYWCNEICGT"
     gene            complement(70..636)
                     /locus_tag="ACHLCY_005391"
     CDS             complement(70..636)
                     /locus_tag="ACHLCY_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0372390.1"
                     /translation="MAKPARRRCNRKREDLTVKRIFELLSFDKSTGVFRWKVPTQGRI
                     ALNSVAGTFDSNGYSMIMIDGRRYKTHVLVFYITHNRWPAGQIDHVNGIRTDNRPENL
                     RECLPIENSRNIRIRKNSKSGCRGVTWHKRQKKWNVRLGFHGKSKHFGCFDDLELAVL
                     VAEEARDKYYGDFSGNERSTYANLSKEM"
BASE COUNT          337 a          235 c          169 g          328 t
ORIGIN      
        1 ttggttccac aaatctcatt acaccaatat tgattttgat attttggtat aaaccattca
       61 ttgcaacatt tacatttcct tcgatagatt cgcataagtg ctcctttcgt tgccggaaaa
      121 atcaccgtaa tacttatctc gggcttcttc agcaactagt accgctaact ccagatcatc
      181 aaagcatccg aagtgtttac tcttgccatg gaatcctagc ctaacattcc attttttctg
      241 tcgtttgtgc caagtaaccc ctctgcaacc tgatttgcta ttctttcgga tccttatatt
      301 tcttgaattt tctattggca ggcattctct taaattttct ggcctattgt cggtcctaat
      361 tccattaacg tggtcaattt gaccagcagg ccaacgatta tgagttatgt aaaaaactaa
      421 gacgtgagtt ttatatctac gcccatctat catgatcatt gaataaccgt tggaatcaaa
      481 agttccagca acactattta atgctatcct tccctgagtg ggaactttcc atctaaatac
      541 cccggtagat ttatcgaaac ttagtaactc aaatatcctt ttaacagtta aatcttctct
      601 tttacggtta catcgtcttc gcgctggttt agccatcgcc ttcttcctcc gtaatggttt
      661 tctgaatttg gccacctgaa cagagctcac caaagctatg gatgtcggta tttccacaat
      721 accaagatgg cgaaaataac tgcatgataa gcctcaggga aaagggaaga cactaccccc
      781 gataattcag aaacaaatcg aaatacatga actaaagaaa aaaccacaac aaacagaaat
      841 agaaatagaa atagaaatag aaatagaaat agaaatatta aaacagacca ccacattcct
      901 gatgtcatac tcactaaaca atttttcgac aacatggtag ctcatagaac gttatcgtgt
      961 agacaccctc tgctacgtat tcggtgttca gtgcaatatc tacaaatact ggaaaaatct
     1021 aaaatcgagg caagtttttg gtgataacta tagttagact atattgacg
//
LOCUS       DAZGTR010000397         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGTR010000397 DAZGTR010000000
VERSION     DAZGTR010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.401"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(321..503)
                     /locus_tag="ACHLCY_005392"
     CDS             complement(321..503)
                     /locus_tag="ACHLCY_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0372391.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRYRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            660..1004
                     /locus_tag="ACHLCY_005393"
     CDS             660..1004
                     /locus_tag="ACHLCY_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372392.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
BASE COUNT          288 a          257 c          224 g          290 t
ORIGIN      
        1 atctttataa attctcatat caggcttgca cccgataaac cgacgaaaac tatttaaaac
       61 ccatcgagtg aagtaatctc taaaaccaaa gaaataccaa gtgaaaatat tcacgataaa
      121 atgcccggtc aaagcccctc ctgtaccgca tgcaagaaca gtaaaaaatc agatgttttc
      181 ataaatatca gtcctcatcg ttttgcctgg catgtccttt accagcaatc ttctgtatgc
      241 actaagccta gatagaatcc actcagtgta cactgaagcc cgctcgacgc tttcttgttc
      301 gtaacttcga ttttagtcaa ttaccttgtt ttcctcgcac gatgtcttag ccaccggata
      361 tcccacaggt gagccgtgta attgaaggtt tttacgtcag attcttttgg gattggcttg
      421 cgtttatttc tgtagcgttt cgttggaagg tatttgcagt tttcgcagat gatgtcggtg
      481 aaacttcgtc gctgtcgcct catgccgccc tcctgacgcc ctgcccgatc gccatcaatg
      541 ccgctttgga tcagttcgcg ataaaggtga atatcggcaa tgaataacaa tcctcgcact
      601 cgcggggatt tcttttatct gaactcgcta cggcgggttt tgttttatgg agacaagaaa
      661 tgtcagattt ggctatgaag gttttgaaat ggcaatcgac tggcgatgtt ggcatcagta
      721 gcgcaactct tgcctcaatc gcatgtggac tgaaaaagaa tatctatggt catcacttcg
      781 gcgctccaca tgacgcagca gacttccggc gatgcgttgc acttgttgag cagattccag
      841 aaatcagaga ttcattcgac aaggttgcaa agcgcgttcc ggcattcaaa ggcatcctca
      901 acgaatggga ttccctcgtc gctctgttga agtctgaaat gaagatacac ggaaacaaag
      961 caccagagac ttacagaaga attagcgagc tacgcaagga ctaaccacag cctcacactc
     1021 aatgaggcct gttcatttct caagatatcc agacctacc
//
LOCUS       DAZGTR010000398         1055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGTR010000398 DAZGTR010000000
VERSION     DAZGTR010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.402"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..557
                     /locus_tag="ACHLCY_005394"
     CDS             <1..557
                     /locus_tag="ACHLCY_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP0372393.1"
                     /translation="SDLKETTSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPS
                     TQVGQKVLDKILNYTNTEMKEAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYE
                     VVPVALVVAGTQMATGHRTMDTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPM
                     AFENIKQVIDDMATDATMFDVNKK"
     gene            complement(656..1055)
                     /gene="tnpA"
                     /locus_tag="ACHLCY_005395"
                     /pseudo
     CDS             complement(656..1055)
                     /gene="tnpA"
                     /locus_tag="ACHLCY_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          297 a          239 c          250 g          269 t
ORIGIN      
        1 actctgattt aaaagaaaca acctcagcaa caggtaaacc tgttttacgt tgggtagacc
       61 cgagttttga tcaaagcaaa tatgacagca tcgtctggaa cccaatcact tattatccgg
      121 tgccaaaacc ttctacccag gtagggcaga aagttctgga taaaattttg aactatacca
      181 acaccgaaat gaaagaagcg atagcgcagc gtaaaccact ggttaccacc gctgggccgc
      241 gtagtctgat tttccgtggg gccattaccg gtgtagatac cagcaaagaa gggctgcaat
      301 tctatgaagt ggttcctgtt gcattagtgg ttgcggggac gcaaatggct acaggccacc
      361 gtaccatgga tactcgcctc tattttgaag gtgagctgat tgatgcagcg actaataaac
      421 cggttatcaa agtcgttcgt cagggcgaag gtaaagacct gaataacgaa agtacgccaa
      481 tggctttcga aaatattaaa caagttattg atgacatggc gaccgatgcc accatgtttg
      541 acgttaacaa aaagtagttc cagacgcgcc atcgttagat ggcgcttttt atccggtgtg
      601 ccgtaaaacc ccatccttca gggcggggat ataaggtgcg gttttccacc tgaccaggtg
      661 tttgctgttg ttcaatgtat tggcggatga cggatattgg cgcacctccg cagctactgg
      721 caaaatagcc aggactccac aaaacgcctt tgtagtaata cctgactgca atatctggtc
      781 gatctcgtcg cagtaaccta cccgatacgc ctttgaggct gtttaccaga ctggatatcg
      841 ccagtttggg aggttagtta attaacaaat ggacgtgatc tggttcgcca tccatttcaa
      901 ccagttcagc ttcaaaataa gcacatacat ttgaaaagta agtgcgtagt ttttctgtcg
      961 cgtcaaaaaa tctggcgtcg gtatctggtg acaaagacca ggtgaacatg catcaggaaa
     1021 acacaatgcc ttccacgccg gatatcggtt tcttt
//
LOCUS       DAZGTR010000399         1053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGTR010000399 DAZGTR010000000
VERSION     DAZGTR010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.403"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            48..362
                     /locus_tag="ACHLCY_005396"
     CDS             48..362
                     /locus_tag="ACHLCY_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0372394.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     KC"
     gene            complement(482..574)
                     /locus_tag="ACHLCY_005397"
                     /pseudo
     CDS             complement(482..574)
                     /locus_tag="ACHLCY_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
     gene            616..939
                     /locus_tag="ACHLCY_005398"
                     /pseudo
     CDS             616..939
                     /locus_tag="ACHLCY_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          272 a          221 c          317 g          243 t
ORIGIN      
        1 ggaaggtaaa ttcaggatgg cagtctgtag ataatcggag gtcacttatg ctacatgatc
       61 acgtggcaga atgtctggag aaaaaaggac tgtaccggag agcagctgaa cgatgggcaa
      121 aagtgatggt acagctaagt gatgaccaga aaagaaaagt ggcggcacag aaacgagcag
      181 agtgtttgcg taaggcgcgc cggactccgg tttcaccggt gaacctgacc gaaataaaac
      241 aagcggtcaa cagactacat tctgagttgg gaatgggatt tgaagagcgg cgggtattcc
      301 gacgatataa agggacagga gaacagaata cgtccggaaa cgcgcggtca aaaaaatgct
      361 aaaaaatatc tgagagagtt attgcctgtt accataagaa aaagcgactt tagtggtcgc
      421 tttttgtgtc atatataagt cgtttaagta aacctgtctg aacaggtgct ctggtcgtgt
      481 tctggtcaag agcgatgtta atttgttcaa tcatctggtt tggaaatcgg atgttgcggg
      541 ttgttgttct gcgggtccgg tttttcggtg acattttctt tcctctggtg acaagctata
      601 tggcgaggat tttacatggc cgtgcttcgt acgttaccgg gcagaatcaa aactctgaat
      661 acccggcggg tgaatgtcct gaagggtgaa cagcgtcggg tcagtggcag tgcccgggtt
      721 tccctcaagc gtcgtatctg gctgagggat gccggacagt gctgtctctg tgggcgtgtg
      781 gttgaccttt gtgacagtga actcgatcac cgcattgcac ttcagttcgg tggtggtaat
      841 gaggagacga atctctggac gctctgtacc gaatgccatc gccagaagtc agtcagtgaa
      901 acggcgagtg gtatgccgga cccgacgctg ccggaggtca tggcagggca gacgatatca
      961 tcggactgtg acccgccccg ggggggggga tcatccggcg aaaaaaacga tcgccccgga
     1021 caccgccccc cgtctcatgc agagaaaaaa ttc
//
LOCUS       DAZGTR010000400         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGTR010000400 DAZGTR010000000
VERSION     DAZGTR010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.404"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>1046
                     /locus_tag="ACHLCY_005399"
     CDS             <1..>1046
                     /locus_tag="ACHLCY_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0372395.1"
                     /translation="TVEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEGSRLVT
                     TAITADTEHRSSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTPGYF
                     QITAVPRLAVYDPTVQFEFWFSETKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDF
                     WFYVRSVNLVGKSAFVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADE
                     MAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDG
                     IPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLK
                     RLFAVSITSSGNPPA"
BASE COUNT          260 a          268 c          323 g          195 t
ORIGIN      
        1 acggtggagg tgagcgcggc tgacggccag tatctggcac aggcgaaatg ggacacgccg
       61 cgggtggtga agggggtgcg cttcagtctg cgactgacca gcggaagcgg agaaggcagc
      121 cgtctggtga ccaccgccat caccgcggat acagagcatc gttccagtgg tctgccgctc
      181 ggggaataca ccctgacagt cagggcaatt aacagctatg gccagcaggg cgaaccggcc
      241 accaccacct tccggattaa cgcgccagca aaacccgcca ccattgaact gacgccgggg
      301 tattttcaga taacggcggt cccgcgtctt gcggtgtatg acccgacggt acagtttgag
      361 ttctggtttt cggagacaaa aatcgcagac acatctcagg tggaaacctc tgcccgttat
      421 ctggggaccg gcagtcagtg gagtgtatcc ggcccgcaca ttaagcccgg gaaggatttc
      481 tggttttacg tgcgcagcgt caacctggtg gggaaatctg cgtttgtgga agtcagcggg
      541 cagcccagca atgatggtga agggtatctg gaatttttcc gggaaaaaat aggaaaactg
      601 catctggctc aggggctgtg ggagctgata gacaacagcc agcttgcgga tgagatggcg
      661 gagatgaaga ccaccatcac cgaaacccgc aatgaaatca cacagacggt cagtaaaacg
      721 ctggaagacc agagcgccac cattcagcag atacagcgcg tgcagaagga cacaaatgat
      781 gacctggctg cgctgtacat gctgaaggtt caaaaaacga aagacggcat tccctatgtg
      841 gccgggattg gtgcagggat tgaggatact gatggccagc cactgagcaa catactgctg
      901 ctggctgacc gtatcgcgat gataaatccg gagagcggca acagcacgcc gttatttgtg
      961 gcgcagggga atcagctgtt catgaacgac gtgttcctga aacgactgtt tgcggtgagc
     1021 atcacgtcat ccggcaatcc tccggc
//
LOCUS       DAZGTR010000401         1045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGTR010000401 DAZGTR010000000
VERSION     DAZGTR010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.405"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..153)
                     /locus_tag="ACHLCY_005400"
     CDS             complement(<1..153)
                     /locus_tag="ACHLCY_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372396.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLG"
     gene            347..772
                     /gene="yqeK"
                     /locus_tag="ACHLCY_005401"
     CDS             347..772
                     /gene="yqeK"
                     /locus_tag="ACHLCY_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFP0372397.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNTEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            808..888
                     /locus_tag="ACHLCY_005402"
     CDS             808..888
                     /locus_tag="ACHLCY_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372398.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(921..>1045)
                     /gene="yqeJ"
                     /locus_tag="ACHLCY_005403"
     CDS             complement(921..>1045)
                     /gene="yqeJ"
                     /locus_tag="ACHLCY_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFP0372399.1"
                     /translation="QLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
BASE COUNT          331 a          156 c          184 g          374 t
ORIGIN      
        1 tccaagagat tcttgttcaa tataatttaa accagacccc attgtgattt taactggatt
       61 aacggatttc tcttcatttt tcttattttt gttctcctga tcaggaacta atggttgcag
      121 tggtaggcta ttattaatat ttcgtggctt cattttacat ttctctcatt tgattagagc
      181 aaaatccgaa ttcgcagctt ctgataatgt caaatatcac cttatggaga cgttacgtag
      241 aatgactata tttgttatcg gcacattagg ggtattaatt ttagtattga tggaaggtga
      301 ttattgaaca gtaacatcga gtcacggtaa aaatgtgagg tatataatgg acattgagtt
      361 ttcgcagatt catgaaatgg tctatatgca tgatattgta aattcggact cgaaaaagaa
      421 accaaggatt ccgttaaaaa aattccttaa tactgagaag gttctcacgc aaacaacatc
      481 atgggcatta aattcaagat ttgtgaatgt taattctgtc aataaagtca acgtaaagag
      541 taaagttaaa agcagttata tctcacgatc tgtcaatgat gagtttagtt taacagatga
      601 tgaaatcaat tcttttaaag aaacgttagt cctttcctct attgattcac tgagtaaact
      661 ggttcttaat aatcctcttt ctgtattgtt tacatcgact gtacgaagga ataataatcg
      721 agctaagatg aatgtcgagt tcgactcatg gatttgcacc agatgttgtt gatacatgat
      781 attttacatt gcaaggagat gtctaaaatg aaagatgttg atcaaaactt tgatgcttta
      841 gactgccata tactgcgaga atatttaatt ttattgtttt atgattaacg aggttccatc
      901 tttttgttat ctattttagt ttaccttgca caatgcatta ttgctctgtt accaatgctc
      961 aaaataaaag catgcttacc ttgctttagt atattatagc ttacactttt ttctggaaag
     1021 acataaaaat caggaataag ttgtg
//
LOCUS       DAZGTR010000402         1029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGTR010000402 DAZGTR010000000
VERSION     DAZGTR010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.406"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..452
                     /locus_tag="ACHLCY_005404"
                     /pseudo
     CDS             1..452
                     /locus_tag="ACHLCY_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(471..1029)
                     /locus_tag="ACHLCY_005405"
                     /pseudo
     CDS             complement(471..1029)
                     /locus_tag="ACHLCY_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          349 a          193 c          166 g          321 t
ORIGIN      
        1 attacaaaaa tgtctttcgc cagcaactct tgctctaatg aagcaggaac aatgttcaga
       61 atcgaaccaa atttaattaa agctatcgct cttgttgaaa gcaatctaaa aaaggatagc
      121 ataggcaaga acagagataa gaataacaat ataaagagtt tagattattg gttaatgcag
      181 ataaaccaga tgcatattcc cctgttaaaa aaacgtggaa taataaaaga tgaacgagat
      241 ttgttagata acccttgtct gaacattaaa ataggaaccg agattttata taaccatttt
      301 tcacgttgtg gagtaacgtg gcaatgcctg gggacttata atgcgggttt tgcaatggat
      361 aaccaaaaaa agagacagca atatgcccca aaatatacat tgtatatacc aggcttaatg
      421 aactagacaa tcgtaaggct tcagcaaaat gagcctaaag cctctttttt ttatatagtg
      481 caaacaccca tacgtaaagc tgttttgatc aactcgatac cactgtaaac atctaatttc
      541 ttcatgatat tgagtctatg agtttcgatt gtttttctgc ttaactgcaa gaaattagcg
      601 atttctttgt ttgttttacc atcagctatg agttgtaata tttctgactc acgatttgta
      661 agttgattat cagaggaata cctggaagat atttttccac aatccatatg tacactatca
      721 aaataagtgt aaccattact aatcgaatca atagcacgat ttagtttcgc cgggtgactg
      781 gttttccata cgcatccttt cgcacctgct tcaagcaatt ttattgaatc aatatacggc
      841 atctttgcta caaatgtaat aatatgagca tctatcttat gcgatttaac ccattttacg
      901 agttcggacc ccatcattcc acctgcaaat tctcctaaaa aaaatattta ggctgtagat
      961 ttgttacact ccgctatagc tttcttcaga tctttatagc ccccaacaac ctcatataaa
     1021 tcaggataa
//
LOCUS       DAZGTR010000403         1014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGTR010000403 DAZGTR010000000
VERSION     DAZGTR010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.407"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..993
                     /locus_tag="ACHLCY_005406"
                     /pseudo
     CDS             1..993
                     /locus_tag="ACHLCY_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1203 family transposase"
     misc_feature    61..177
                     /locus_tag="ACHLCY_005406"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          254 a          241 c          302 g          217 t
ORIGIN      
        1 agagctggaa cgtgaaaatc gtgaactgcg ccgcagtaac gatatccttc gccaggcttc
       61 cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa aaatgatgcc actgctggat
      121 aagctgcgtg agcagtacgg ggtcggaccg gtatgcagcg aactgcatat tgccccgtca
      181 acgtattacc attgtcagca acagcgacat catccggata aacgcagtgc ccgtgcgcag
      241 cacgacgact ggctgaagag agagatacag cgcgtatacg atgaaaatca tcaggtgtac
      301 ggtgtgcgta aagtctggcg tcagttgtta cgggaaggaa tcagggtggc cagatgtaca
      361 gtggcacgtc tcatggcggt tatgggactt gccggtgttc tccggggtaa aaaggtccgt
      421 acgaccatca gccggaaagc cgttgccgca ggcgaccgcg taaaccgtca gttcgtggca
      481 gaacgacctg accagctgtg ggtggctgat tttacttacg tcagcacatg gcagggcttc
      541 gtctatgtgg cgtttatcat tgatgtgttt gccggataca tcgtggggtg gcgggtctca
      601 tcgtctatgg aaacgacatt cgtgctggat gcgctggagc aggcgttgtg ggcccgtcgt
      661 ccgtctggca ccatccatca cagcgataaa ggctctcagt atgtgtcact ggcctatacg
      721 gagcgactaa aagaagccgg attactggca tcaacaggga gtacaggcga ctcgtatgac
      781 aacgcgatgg ctgagagcat caatggtctt tacaaagcgg aggtaataca ccgtaagagc
      841 tggaaaaacc gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat
      901 cgacgattgc tgggaaggct gggccatact cctccggcag aagcagaaaa agcttattat
      961 gcttccatcg gaaacgatga tctggcagcc tgagttcaca gataaaacac tctc
//
LOCUS       DAZGTR010000404         1003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGTR010000404 DAZGTR010000000
VERSION     DAZGTR010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.408"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..795)
                     /locus_tag="ACHLCY_005407"
                     /pseudo
     CDS             complement(1..795)
                     /locus_tag="ACHLCY_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556527.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          309 a          197 c          202 g          295 t
ORIGIN      
        1 ttacagaacc tgaagtcact gtggaatcgg taactgagat attgttatcc caagtatgag
       61 attcagtagt gtaagtattg tttacggtat taccctgagt ctgataccag caacgcggga
      121 gttgttagaa atattgatgt tactttcgac atcaagattc actgccagcc caaggccaac
      181 accaccgttt gcaaacaaac cggcgtattc ttcaccttca ccagctgggg tatggaaggt
      241 ttcgttagta tatttggtaa cgtcaccgtc cagatatgaa tcagtgaagt ataaaccttc
      301 ataatcgtca tcaattgtag aattagcaat atttaaagtg aatacatcac catcatgcca
      361 attatcatca attacacgat cagtaccgta ttcagagaac tgatagacat aatgaccatc
      421 actgctttca cccataggca gcattgaagt gattgaacca tgaatcaggg agttgctgat
      481 gttcaactgg tttgttattc catcagtaaa gtcatgattc aggtaatatg tagagatagt
      541 cccgttaaca gtgtcaccat taaaggtggt atcgaaatca ccgtttgtgt tgttatagta
      601 gacaaagcca ctataattca catagttacc atctacagta cccggtgcat ccatattgct
      661 ccagtcaaga gcggtagttg gattgtcgta ataagtccag gtgtcataag tagttgcatt
      721 agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta caagtttctt
      781 tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc catcaattat
      841 caatgcatag aaaacatatt atgtttcgcg ctaatgatta tgctgcgtta cagacgaatg
      901 gttcaattat tctttcccct aagtccgaat gtggacatat ataacctatg gagaaaaaca
      961 cgcatcaaat tgataaaagg gcgatatttc aacatgttgc aga
//
LOCUS       DAZGTR010000405          997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGTR010000405 DAZGTR010000000
VERSION     DAZGTR010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.409"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..97
                     /locus_tag="ACHLCY_005408"
     CDS             <1..97
                     /locus_tag="ACHLCY_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372400.1"
                     /translation="RRTHSVWRHRHWHASAFTGICNDLAAASACT"
     gene            94..675
                     /locus_tag="ACHLCY_005409"
     CDS             94..675
                     /locus_tag="ACHLCY_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0372401.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
BASE COUNT          234 a          242 c          316 g          205 t
ORIGIN      
        1 acgacgaacg cattctgtct ggcgtcaccg ccactggcac gcatctgcct tcacggggat
       61 ttgcaacgat ttggccgccg cctcagcctg tacgtgaaca cggcagcgga agccatccgt
      121 gccctgtcgc tgcagatgcc gggattccgc cgtcagatga acgaaggctg gtaccagata
      181 cgtattcgcg gtgaggacac ggcaccggag gcggtgtacg cccgtcttca cgaacagctg
      241 ggtgagggaa cggtcatcca cattgtgccg cgactggccg gggccggaaa gggtggactg
      301 cagattgtgc tgggggcggc agccatcgtg ggctctttct tcactgccgg ggcatcgatg
      361 gcgttatggg gttcagccct ggcagccggt ggtttttctg ccaccacgat gctgttttca
      421 ctgggtgcca gcatgatact gggtggtgtg gcacagatgc tggccccgaa gccaaaaaca
      481 ccggaataca gggcaacgga taacggtaaa cagaacacgt acttttcgtc gctggataac
      541 atgattgccc aggggaaccc gatgccggtg ccttacggtg aaatgctggt tggctcacgg
      601 cgaatctccc aggacatcag tacccgtgat gaaggcggtg acgggaaggt ggtggttatc
      661 gggcggcagg ggtaaagcat aaaaaaatcc cgcagtgtat ggaggctgcg ggaacagaaa
      721 atgaagatta accacaggga gttttgtttt tattggcccg aaaaaactgt aacgcccggg
      781 aatgatatct gccacggggg cgtacagaaa atgtgaagaa attcagaaat tttattccgt
      841 catgacacag gcaccctccg ggatgcctgt tgtttttgtg cgtaacagtt atcacagtaa
      901 agggtgagac aatgggcaaa ggtggtggca aggcgcacac gccggttgag gcaaaggaca
      961 atcttaagtc cacgcagatg atgagcgtga ttgatgc
//
LOCUS       DAZGTR010000406          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGTR010000406 DAZGTR010000000
VERSION     DAZGTR010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.410"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(210..881)
                     /gene="queE"
                     /locus_tag="ACHLCY_005410"
     CDS             complement(210..881)
                     /gene="queE"
                     /locus_tag="ACHLCY_005410"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP0372402.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          274 a          244 c          209 g          264 t
ORIGIN      
        1 ttaaaataac tctatacaaa ctaaaatcta ccaaacttta ccgcaataat tttcactcca
       61 gcgaaaaatt aatgccacag aatttgtaga gaatgctaat gattagccct ggatgggtaa
      121 aatggaggag ttttcagagg cgacaaacaa tataaatgag tagaagtctt tacaggttcc
      181 acccatttat aacgcttata aatgtttaat caggcaatat ttagatattt atgtgtttgc
      241 atcgacaaac gccaattacg cgcaatgcag gtttcaatgc acaaacgtgt ggcatcatcc
      301 ttttggctaa tcggctgcag tgcaatgact cgcggtttat catcggtcag cgttgccagt
      361 agttcatcca gtgcttcaat atcacgtacg cgccccactg gatgcttgat ttcgttggct
      421 cgctccagtg cctgtgacaa cacgtcatag ccgccgcgca tgttcagctt tggcgatacg
      481 gtaacccagg tattcggtgt gcagcgtact tcatgagttc cactggtttc gatctggcag
      541 ctaaaaccgt tcttttcgag caggtcagtc agtggcagca aatcatgaat gcaaggctca
      601 ccacccgtaa tcaccacatg ccgcgcggta tatccctgcc gaccaatgac agccagcaaa
      661 tcttcactgc tcgccgcccc ccacttatca ctctctttgg tcttcgccag aatgctgaaa
      721 agggagactt cccgatcctc aagcttttcc caggtgtgtt tggtgtcgca ccaggcacag
      781 ccaaccgggc atccctgtaa acgaataaaa atggcgggga cgccggtaaa gtaaccctca
      841 ccttgcaggg tctggaacat ctcgttaatc gggtactgca tagcattctc tgtgaagtgg
      901 ataattgtta attattgcag atcctgccac aacaatcatg tcttattaac attctgttac
      961 aggcagattt aaagaaaaat gcgaaacata t
//
LOCUS       DAZGTR010000407          969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGTR010000407 DAZGTR010000000
VERSION     DAZGTR010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.411"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..868
                     /locus_tag="ACHLCY_005411"
     CDS             <1..868
                     /locus_tag="ACHLCY_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0372403.1"
                     /translation="QLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSY
                     LALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGD
                     AIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIE
                     IIYIKRPSKDLANLRKEFNKTVRKNFLIKLAKTSEASGRFNAEDLLRMRKGNVPLNYN
                     VHHKLSLDDGGTNDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSI
                     YPPMKNIMDHTK"
BASE COUNT          380 a          170 c          155 g          264 t
ORIGIN      
        1 acaactaaga ggcgcttttt tatcggctat tcatcaccag atcattgaaa accaactgga
       61 tacagaaaca ataactatta gttatgaatt tatcctctgt caacatctta tcgcaaatac
      121 cgagtttagc tatttggcac tccctgaaaa ttataaccgt ttgtttttac caaattcaaa
      181 aaaccaaaca aataatcgtt tcaaaacgtt aaacagcaaa gctattggca ggctacttgc
      241 tgctggtggc gtatacaatg ggaacattga aggattcaga gatactgcgg aaaaactggg
      301 tggagatgca ataaaaggct atgatcaaat actaaatgaa aaaacagcgg gcatagcgat
      361 agcaacagca tctattcttt taacaaagcg ttctaatgtt gatacatata cagaaataaa
      421 tagttactta ggcaaactta gaggtcaaca aaaacttctt gatggtatag acataataga
      481 aataatatac attaagagac cttcaaaaga cttagctaac ttacgaaagg agtttaataa
      541 aactgtaaga aaaaattttc ttatcaaact tgcaaaaacc tccgaagcat ctggaagatt
      601 caacgccgaa gaccttttaa gaatgagaaa gggcaatgtt cctctaaatt ataatgttca
      661 ccataaacta tctctagatg atggtggtac taatgatttc gaaaatttag tattaatcga
      721 aaacgaacca tatcataaag tttttactaa catgcaatca cgaatagcta agggaatatt
      781 agtaggtgaa agcaaaatca ctccctgggc cattccatct ggctcaattt atcctcccat
      841 gaaaaatatt atggaccaca caaaatgatg actaaaaccc aaataaataa attaataaaa
      901 atgatgaatg atttagacta tccatttgaa gcaccgctca aggaatcatt tattgaaagt
      961 ataatccaa
//
LOCUS       DAZGTR010000408          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGTR010000408 DAZGTR010000000
VERSION     DAZGTR010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.412"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..140)
                     /locus_tag="ACHLCY_005412"
     CDS             complement(<1..140)
                     /locus_tag="ACHLCY_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0372404.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQG"
     gene            complement(121..534)
                     /locus_tag="ACHLCY_005413"
     CDS             complement(121..534)
                     /locus_tag="ACHLCY_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0372405.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFSGLMY
                     AVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGDGGRD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
     gene            complement(561..>968)
                     /locus_tag="ACHLCY_005414"
     CDS             complement(561..>968)
                     /locus_tag="ACHLCY_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0372406.1"
                     /translation="EQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHDVVHIN
                     QRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATNRVLYL
                     SGMLDNRHDADPEQTGKAEATEPVTSKKHSKAS"
BASE COUNT          223 a          294 c          224 g          227 t
ORIGIN      
        1 ccctgttcaa cagtactggc ggtttttctg gcatccgcct ccagactgga aaaatgacgg
       61 cgtacccggg ccatctgttc atcaaaccgg gccgcatcca gactcaaatc aataaccaga
      121 tcacccgctg gctgggacat atctcacacc tccggaaatc cccgctgaag ccatcattaa
      181 tgcaacatca tcctcgctga catccaccac atccgcagaa ggtgaaatat cgcgccctcc
      241 gtcccctccg aaccggacgc ctccggcaac tcctgtcgct ttctgcatca gcatttcttc
      301 ctcgtccggc atctccgtct gctcttcctc acacgctgga gcaagcagac tgaaatccgc
      361 cggatgcata tccggatcgc caaaaaacag gctgagtacg gcgtacatca gcccggaaaa
      421 atgagcgtcc agttgggtat cctgaaaata atgcgtgcag taaaaacgtc gccagtcggc
      481 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctctcg
      541 cgccagtttc aggacaaagt tcagctcgcc ttcgaatgct tttttgatgt taccggctca
      601 gtcgcttctg ctttcccggt ttgttcagga tcggcatcgt gccggttatc cagcatacct
      661 gaaagataaa gcacccggtt cgttgcctga ttcagtgcat cagcaggcca tcccagcatc
      721 acttcacggc ggatctgctg catctctgtc tccggagagg ccagagtgcc tttgagggaa
      781 tgggaatgcc atagcgacat cgccacaagc agggatgccg tttccagata tcgctggtta
      841 atgtgcacga catcatgctt cgttgtctcc tgttgttctg cgtctgaaac aaactttata
      901 taatcaaacc gctgcagcgc agacaattcg gaaagcgtga cggacacacc gttatattca
      961 aattgttc
//
LOCUS       DAZGTR010000409          959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGTR010000409 DAZGTR010000000
VERSION     DAZGTR010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.413"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            207..569
                     /locus_tag="ACHLCY_005415"
     CDS             207..569
                     /locus_tag="ACHLCY_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination N domain protein"
                     /protein_id="HFP0372407.1"
                     /translation="MGQEEKYELKKLIEEDAIEEIAALTTAIKNIRYALNTLISSCDK
                     NSREFLILGAALGIVDAATLHLITHDDILIEPYETLLLVRQKMADAAANGDLQLYIDL
                     RKVLRRMVRTEGDITLTK"
     gene            750..>959
                     /locus_tag="ACHLCY_005416"
     CDS             750..>959
                     /locus_tag="ACHLCY_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372408.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWL"
BASE COUNT          313 a          180 c          224 g          242 t
ORIGIN      
        1 aagccatgtc gaacggcgcg acattaaacc atgcgtcggg agcgcggcgg gttcaggatg
       61 aacggcaatg ctgctcacta caaaagaaca tttccataac ttgatagtta ttaaagtgtt
      121 tttatatacg gggttagcga tctcgtgaca caaaccattt acgcggcagg ctaccggaaa
      181 tcaaaacaat tgacggcgag gtaattatgg gtcaggaaga aaaatatgag cttaaaaagc
      241 tcattgaaga agacgccata gaagaaattg cagcattaac aacagctata aagaatatta
      301 ggtatgcgct aaatacgctt atctcctcat gcgacaaaaa tagcagggaa tttttgatac
      361 ttggcgcagc tctaggaata gttgatgcgg caacgcttca cctaattact catgacgata
      421 ttcttattga gccgtatgaa acattactgc ttgtcaggca aaaaatggct gatgccgcag
      481 caaatggaga ccttcaactt tacatcgact taaggaaagt attaaggcga atggtcagaa
      541 ctgaaggaga tatcaccctg acaaaataag ggggtgagag gattttacta tttttctcgc
      601 tgtaggggta cacgagaacc accgagcctg atgtggttaa aagacaggca caatctttac
      661 taccgcaagc cacgcagtga aatgggtgta acttgtgttg gtcgccagaa aatgaaatta
      721 ggcagcaaac cacttatttg aggtgagata tgacaaaatc atggagcgta ccttttcctg
      781 aatcagaaac tgaacatgat ggaatgcctg ttttctggag attccaggcg acagttgaag
      841 aagatgggat aaaaatattc gcacttcaat atatagcttt tcatcagaca gagcattatg
      901 catggttggt tcctgcgcat tggattgtta attttaaacc agcaccaaat cagtggtta
//
LOCUS       DAZGTR010000410          951 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGTR010000410 DAZGTR010000000
VERSION     DAZGTR010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.414"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..951
                     /locus_tag="ACHLCY_005417"
                     /pseudo
     CDS             1..951
                     /locus_tag="ACHLCY_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          372 a          185 c          138 g          256 t
ORIGIN      
        1 acatatcacg gcgatattta taactgcaca acaaataccg ataatgctaa aacgccagac
       61 acaccgaagt ggccttgcga taattgggaa gaacaacaac ccattaattc cacattctct
      121 ggagaaggat atatctctga tcaatatgat ttagcgcaac accaacttca acaaattaat
      181 gcgtgccaca cgaacactac atatacaaat gcagactact caaaagttgt ggctcaactt
      241 gtaagtctta ttactaacat tgaaacgata agttcgacat aacttacgca acagacacaa
      301 tcaatactta atcagattaa taacattaga tatgaaaaaa acaaaagtgc tgaatgtcga
      361 atcattgttg tcgctaatcc taaacccgac aaggcaatca taactaaaat tagcgtagaa
      421 gagggaatac ccataacatt cagtgtccaa accatgtttt cagatactaa ttttattgct
      481 gaacaacgag ctgacttacc aactaatata aaagatattc aaagcctgta tcaaaaaatg
      541 acgaagttat atattgagca cagtgaaaat aaaaacagga tgaaagtctt tgccggaacc
      601 aactttattg atttcaatat gacaggacag aacctgtccg ggttcgttct aaccttgtca
      661 aggttttatt tcgaagatct acttaatata aatttcactg acgaaaattt aggtgacact
      721 attttttaca taaagaacac cctaccccca aattatataa agatggacaa tatcttgaca
      781 aacaaatcga aggtttattt tcaacattat taaccattaa tgacaacttt ttacgagcaa
      841 aagcggaaat tgcttcgaca ataattaaat ttttagaggc caggataacc aatctttcat
      901 acaatgacat attaacatat cagcaagaat tccaaaaaca atgttataag c
//
LOCUS       DAZGTR010000411          946 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGTR010000411 DAZGTR010000000
VERSION     DAZGTR010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.415"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..483)
                     /locus_tag="ACHLCY_005418"
                     /pseudo
     CDS             complement(1..483)
                     /locus_tag="ACHLCY_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          257 a          205 c          213 g          271 t
ORIGIN      
        1 tgcgtttcct gatggcatgc cggatcacac tgttcagcga ctcgatggcg ttcgttgtgt
       61 agatcacttt gcggatatct gccggataag cgaaaaacgt cgccaagtta gtccagtttg
      121 acagctagca ccggcttatc tgcggatagc ggcagtccca ggccgcagca aacgcttcca
      181 gtgcctgctg gcctgcctct tctgtgggag gctaatcgct ttcaggtcgc gagtgacagc
      241 tttgtattcc ttccatgaca cgaagcgcat gctgttgcgc accatatgca cgatgcataa
      301 ctggatgcgg gcctccggga attctttcag gccatacaca caggcgatga ggatatcgtt
      361 cagaccgcgg tttttcagtt cagtcagcac attgaaccag aaattcgccc cttcattttc
      421 ggccagccgc atccccagga gctctttctg acctgcgata ttgatgccca gtgctaggaa
      481 catttttatg ataaaaaatt agtgcaagaa gacaaagaat cgccttgcgc taatgctctg
      541 aaacaggtca ctaatatcat ctaaatagtt gactcatagt gacagcatgt gttgtgtctt
      601 gcagtattat atagcctatt atttagattg aagccatcat aatctattga tgttaatggt
      661 tttttattgt ttgttgttcg gctttttata ctaatttgag cgaaacaaaa agataaaaag
      721 ttgtttttcg tgtctttaag tgataccaga tggtattgcg ctaaatgtac caatgggtat
      781 accaagagca ctaaaaacaa aggggcaacc actttgtaac cccttgatta tttgcagaag
      841 cgcagaaaat cgaactctgg aaccctttcg gggcatcctt tttcaagaat gaagaaaaaa
      901 ataactaaaa aatcaagttg ttaggaagtt attcatggct agtgta
//
LOCUS       DAZGTR010000412          940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGTR010000412 DAZGTR010000000
VERSION     DAZGTR010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.416"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..851
                     /gene="espL4"
                     /locus_tag="ACHLCY_005419"
                     /pseudo
     CDS             1..851
                     /gene="espL4"
                     /locus_tag="ACHLCY_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT          331 a          181 c          165 g          263 t
ORIGIN      
        1 aaaccattgc cccaatattt gaagaatacc taatggaaat tttacctcaa cgctcagaca
       61 ctcttgataa acaagaatta aacctaaaat cagatagaaa agaaaaagaa ttcccaagaa
      121 ttaaacttaa tggtcaatgt tattttccgg ggcgacccca aaaccgtata gtatgccgac
      181 acattgctgc acaatatatt aatgatattt atcagaatgt tgattacaaa ccccatcaag
      241 atgattactc ttcagctgaa aaatttctca ctcacttcaa caaaaaatgc aaaaaccaga
      301 ctttggcgtt gatttccagc cgtcctgagg ggcgttgtgt tgctgcctgt ggtgatttcg
      361 ggctagttat gaaggcatat ttcgacaaga tggaatcaaa tggcatcagt gttatggcag
      421 ccatattact ggtggataac catgctttga cggtccggct aagaataaag aacacaactg
      481 aaggatgtac ccattacgtg gtttcggttt atgatcctaa tgtcactaac gataaaataa
      541 gaattatgag cgaaagcaaa gaggatatta aacactattc tctgatggat tttatgaatg
      601 tagattatag cctcctgaaa tggtcaaatg atcatattat taaccaatct gttgcaataa
      661 ttccagcact tccgaaagaa cagctattga tgttaaaagg atctgtggat gaaataaccc
      721 ctccattatc accagcaacg atgaatttgc taatggcaat tggtcagaat caccaactta
      781 cgcaactgat gattcagctc cagaaaatgc cagaactaca tagaacagaa atgttgactg
      841 cctataatag tggacatatg aacgttatta atactatttt taacgcatta cccactctgt
      901 ttaatacgtt taaattcgat aaaaaaaata tgaagcccct
//
LOCUS       DAZGTR010000413          930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGTR010000413 DAZGTR010000000
VERSION     DAZGTR010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.417"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..239)
                     /locus_tag="ACHLCY_005420"
     CDS             complement(<1..239)
                     /locus_tag="ACHLCY_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0372409.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGG"
     gene            complement(217..465)
                     /locus_tag="ACHLCY_005421"
     CDS             complement(217..465)
                     /locus_tag="ACHLCY_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0372410.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            complement(530..>930)
                     /locus_tag="ACHLCY_005422"
     CDS             complement(530..>930)
                     /locus_tag="ACHLCY_005422"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0372411.1"
                     /translation="RDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFF
                     VQVWGNGANFDNTILHRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEG
                     ERHNALDDARYQAKYVSVIWQKLIPNQADF"
BASE COUNT          206 a          231 c          221 g          272 t
ORIGIN      
        1 ccgccggtat cactaataat tttctcctgc agactgagca tttgttccat ttgctgccgg
       61 gcaagacggc tgttcgcttc tgctgcaata gtttctgcca gtttctggtc aatactgccg
      121 agaccgtgat ttttgcctgt tatgggatgc ctgtaacgcc agtaaacttt gttatttctt
      181 ttgtcaaaat acggagataa tcccggaaca tcggttttat attttcgcgg gcgcgccatc
      241 ttccagtatc ctcttcaaag cagggtgatc tgtggcgatc acctccggct tgtttaccat
      301 tccgacaaag cgagcttgcg gatccactcg ccagcgtctt ccaacttttt tggggagagg
      361 aaatatcatt ccggctttag cgtatttact taacgtactc ggagttggga ccggttcact
      421 gaattcctct tttgcccact cagtgagcag aataagtctt gccatgagcg tcgttcgcta
      481 atcatggtcg ccgccactat agctggtggg caacgaccgg ggttgaacat taaaaatcag
      541 cctgattcgg gatcagtttt tgccagataa ctgaaacgta ttttgcctgg taacgggcgt
      601 catcaagtgc attatggcgc tcaccttcga atggaatagc cgttctggca tcgaagtcta
      661 tggctttccc cagctcaacg attgtgcgta catcgcgatc gttgtagtaa cgccacgggc
      721 aggggatccc ctgccgttcg tatgaacggt gcaaaatcgt gttgtcgaag ttggctccat
      781 ttccccagac ctgaacaaaa aattcaccgg agttttcgtc gataaattcc cgcaattgta
      841 acagtgcatc atctaacggg atttcatcgg tcataatggc agattgcgct tcgcgtgatt
      901 gcttaagcca ccatttaatg gtgtcccgat
//
LOCUS       DAZGTR010000414          926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGTR010000414 DAZGTR010000000
VERSION     DAZGTR010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.418"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..144
                     /locus_tag="ACHLCY_005423"
                     /pseudo
     CDS             1..144
                     /locus_tag="ACHLCY_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(339..506)
                     /locus_tag="ACHLCY_005424"
     CDS             complement(339..506)
                     /locus_tag="ACHLCY_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0372412.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(503..926)
                     /locus_tag="ACHLCY_005425"
                     /pseudo
     CDS             complement(503..926)
                     /locus_tag="ACHLCY_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          252 a          264 c          192 g          218 t
ORIGIN      
        1 aacatcgtaa tgtttaatcg acatcgaatt tctcccgtgt agaggaacag agttaaaaag
       61 ccggaagcgg aatcaaatca caggatgacc atctgccagt ggctggtcgt aaaaaaaagg
      121 ccgcgccatg cgcagccgaa aataaaggga taacgatgat agtttgagaa aaacagaaat
      181 aacacttttg tggcaaagca tggtgccggg tgcctcccgg tgaattcagt atcagcacct
      241 gaatccgcga ttaccccata ttccttcttg ctgattgccc caccgcacag ggggattcac
      301 catgcagaag tgtttttaat aaacagcaaa caaaaaaatc aagcattatg caggctgttt
      361 ctttttatca ccggccacag caataccata atgccgcaga ccagcacccc atccgccagc
      421 accgacatga ttctgctggt gaaatccacc atcaccacca gaaacagcag gagtgcagcc
      481 acagtcaggc gcagttttac cgtcacaggt aattctccag acgaagaccc agaacaccgg
      541 caatctcttc cagcaccttg cgctcttccg gctcaatttc gccgtctgcc tccgcaatgg
      601 ccaccgccac atccagcaca tcttccgctt cacgcgtatc gtgtttcaca tcctcgatct
      661 cacgtaacgc cgcacgacga ccagttttaa agttcgtatc cagctgaccg ataatggttg
      721 cgctaatcgc attaatttct gacgtaaacg cgtacagcgc aggctgatta cgcagtacct
      781 gttcgatctt cgctttctag gaagcctcac attcaccatc tgcataggcc accaggtagg
      841 cggcgttaat caccacctgt gccagatcgc gtttttcaaa ctttttaatt tccgttgccg
      901 ctctgcgggc tttttttacc aaaaat
//
LOCUS       DAZGTR010000415          926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGTR010000415 DAZGTR010000000
VERSION     DAZGTR010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.419"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>926
                     /locus_tag="ACHLCY_005426"
     CDS             <1..>926
                     /locus_tag="ACHLCY_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0372413.1"
                     /translation="QRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTGLKI
                     SGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLKAMA
                     DDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASGVTK
                     TLSGALNGVAGNIDNVAAAAGVLVAVGVARYFGNMASGAMSATAGLVTAARNEVALAE
                     AQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARTAAQ
                     NALNSTTAVGSRLMTGALGLVGGVPGLVMLGAA"
BASE COUNT          195 a          221 c          329 g          181 t
ORIGIN      
        1 cagcggacag gcacggcatt ttcagataat gcggccctgt ttgcccgctc ggcagcctca
       61 atgcgtgaat atggttacag tgctgatgat gtgctgaagg tgacggaggc catttccacg
      121 gggctgaaaa tctccggtgc cagtacggca gaggcgggct cggtgatcac ccagttcagt
      181 caggcgctgg cgcagggtgt attgcgtggt gaggagttta attcggtcaa tgaaagtggt
      241 gaccggatcg tacgtgcact ggctgcgggt atgggcgtgg cccgtaaaga tctgaaggca
      301 atggcggatg acggaaaact gacagcggat aaagtggtcc ccgcgttaat cagccaactg
      361 gggatattac gtgatgaata tgcggccatg ccggaaacgg tttccagtag tatcacgaag
      421 gtggaaaacg cctttatggc ctgggtgggc ggtgcgaatg aggccagcgg ggtgacgaaa
      481 acgctctccg gtgcgctgaa cggtgttgcc ggcaatattg ataatgtggc cgcggcagca
      541 ggtgtgctgg ttgctgtcgg ggttgcacgt tactttggca atatggcctc cggagcgatg
      601 tctgccacgg caggacttgt gacggctgca cgtaatgaag ttgcactggc ggaagcacag
      661 ttcaggggaa cgcagattgc cacggcgcgg gcaagggcag ccgtgtaccg tgctcagcag
      721 gccgtggcgg cagcccgcgg gacggagatg cagattgcag cagaggcccg tctggcggcc
      781 acacaggaac gcctgaacag aaatattgct gccagaaccg ccgcccagaa tgcgctgaac
      841 agtacaacgg cggtgggctc acgtctgatg actggtgcgc tgggactggt tggaggcgta
      901 cccggactgg tgatgctggg agctgc
//
LOCUS       DAZGTR010000416          914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGTR010000416 DAZGTR010000000
VERSION     DAZGTR010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.420"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            16..914
                     /locus_tag="ACHLCY_005427"
                     /pseudo
     CDS             16..914
                     /locus_tag="ACHLCY_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          231 a          243 c          253 g          187 t
ORIGIN      
        1 gccacctgat gaaaggtgat attctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gaaccttcac ccctgagttc aggctggaat gtgcacagct gattgttgat
      121 aagggctact catatcgaca ggccagtgaa gcgatgaatg tcggttcaac cacgcttgag
      181 agctgggtac gccagctcag gcgagagcgc cagggtatta cgccctctgc cacacccatt
      241 actccagacc agcaacgtat ccgcgagctg gaaaagcaag ttcgccgtct ggaggaacaa
      301 aatacgatat taaaaaaggc taccgcgctc ttaatgtccg actcgctgaa cggttcacga
      361 tagccgccag actaagtgac agccacacgg ttgtcagcct gtgttctgct ctggaaatac
      421 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcacgagtca
      481 ggttgtgcag cgaaatacgc cgggcgtgga accaaagtcg gggctctgcg ggcgcgcgca
      541 ctctggctga aatgctgact caaaacggcg tcccgatgag ccgttaccgt gccggacgtc
      601 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtat aaaaatgccc
      661 gtcaggagca taccagcctg ccgaatctgc ttgagcgtca gttcgcagta ccggagccag
      721 accgggtatg gtgtggagat attacgtatc tctgggcagg aaatcgctgg tgctatctgg
      781 cggttgttat ggatcttttt gcccgcaggg ttatcggctg gagtctgtca gcgcatgccg
      841 ataccgcact gataagcagt gccctgcgga tggcctatga gacgcgtggc cacccccggg
      901 atgtcgtgtt ccat
//
LOCUS       DAZGTR010000417          913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGTR010000417 DAZGTR010000000
VERSION     DAZGTR010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.421"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..178
                     /locus_tag="ACHLCY_005428"
     CDS             <1..178
                     /locus_tag="ACHLCY_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP0372414.1"
                     /translation="PSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVSR
                     RDQESALACWGIDR"
     gene            337..474
                     /locus_tag="ACHLCY_005429"
     CDS             337..474
                     /locus_tag="ACHLCY_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372415.1"
                     /translation="MFDFSKLIREIRMMAEKLSTWKFILIWLVFVIMASGYFIGQIRW
                     W"
     gene            696..881
                     /locus_tag="ACHLCY_005430"
     CDS             696..881
                     /locus_tag="ACHLCY_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0372416.1"
                     /translation="MRELKMKLCALMLPLVVSACGSTPTVQAPCVKPPSPPAWIMQPV
                     PDWQKPLNGIISSSENG"
BASE COUNT          232 a          205 c          265 g          211 t
ORIGIN      
        1 cccgtcgacg ttttacagac ggattaatgc tggtgaccgc aggggagcat gcgaggcgat
       61 tcgctggtgg attaaggacg gtggcagaga ctgccgtatt cgctcaaaca actgctacgg
      121 tcaggtctca cggcgtgacc aggagagcgc gctggcgtgc tggggaattg acagataagc
      181 agaatatttt gctgaaaaat gcggtttgct cacacgggcg gataacacga aatcctgcga
      241 actggcaaaa actaagtgaa taaaagtaaa accccgtttg ttggccgcaa gtggggtttt
      301 gtgtttcctg actccggaaa agtcaaagga gaaagtgtgt ttgattttag caaactgatt
      361 cgggagattc gaatgatggc tgaaaaatta tccacctgga agttcatcct tatctggctg
      421 gtgtttgtga ttatggcttc cggttatttc attggtcaga tacgctggtg gtgaaatgaa
      481 ccgcgtactg tgcgtggtca tcattgccct gctggtggcc tgtggtgcgc ttagtctggg
      541 gctgaatcat taccgtgata acgccataac ctacaaagag cagcgcgata aaaaagtcag
      601 tgagctggag ctggcaaatg caaccattac tgatatgcag cagcgccagc gtgatgttgc
      661 tgcacttgat gccagatact cgagggaatt agccgatgcg agagctgaaa atgaaactct
      721 gcgcgctgat gttgccgctg gtcgtaagcg cctgcggatc aacgccaact gtccaggctc
      781 cttgcgtaaa gcccccatca cctccggcgt ggataatgca accggtcccc gactggcaga
      841 agccgctgaa cgggattatt tcatcctcag agaacggctg atggcaatgc agaagcaact
      901 ggaaggagca cag
//
LOCUS       DAZGTR010000418          904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGTR010000418 DAZGTR010000000
VERSION     DAZGTR010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.422"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..677)
                     /locus_tag="ACHLCY_005431"
     CDS             complement(<1..677)
                     /locus_tag="ACHLCY_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0372417.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTAQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDKPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLS"
     gene            complement(677..>904)
                     /locus_tag="ACHLCY_005432"
     CDS             complement(677..>904)
                     /locus_tag="ACHLCY_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372418.1"
                     /translation="AGLNADLKTYRVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYA
                     YRQIKVTCAGWSARVGMLRVEFSAEFKQVVN"
BASE COUNT          187 a          328 c          207 g          182 t
ORIGIN      
        1 gaaaggaaac cgccaaaatt agccaccatg ccgcgcatct cacacccgcg catgcacttg
       61 ctgcatctgt ccttacggat atcggtggtg ggtttatcga actcatccgc caccgcagga
      121 ccgttatacc cgcattcatc tccccggtaa tcccacatac aggtgttcgc cagcatgatg
      181 cgaccgggaa acagcgctcc gtccgtctcc gtcggtgtgg ccagcacaaa cgaggccgtc
      241 atggccgtca gctctgacat ctgctccacc acccaccggt cagtcagctc ctgctccggg
      301 tcggcctccg gattgcctgc cacaaagttc accgcatcca gaaaacgcgc atacacccgg
      361 cggcggacca ccgtggcacc caccaggctc tgcaaatcct ccgccatccc ggtgacaaga
      421 ccgaacagat tggacaccgt cagcgacggg cgggcactgc tgccctttcc gttcatctca
      481 aagccgctgc cctcaatcgg gtacgcctga tattcacgcc cctgccaggt caccggctcc
      541 cctttttcat tcagctcatt gcagaaaaaa taccgctcac cgccctgcgc cgtcaggtcg
      601 atttcccaga gtaccacccg cggtgactgc tctgatttaa ccgactcgtt cagactttct
      661 tcgtgaatat cctgcatcag ttcaccacct gcttaaactc cgcgctgaac tcaacgcgca
      721 acatcccgac ccgcgcagac cacccggcac aggtcacctt tatctgccgg tatgcatagg
      781 gtggcttcca caaaaatgcc ttccagcctc cgtgctctgc caggaacgct tccagatgcc
      841 gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca gcattcagcc
      901 ctgc
//
LOCUS       DAZGTR010000419          899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGTR010000419 DAZGTR010000000
VERSION     DAZGTR010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.423"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..94
                     /locus_tag="ACHLCY_005433"
     CDS             <1..94
                     /locus_tag="ACHLCY_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372419.1"
                     /translation="LGRRAIGVELETERFEQTVREVQDLVSQNG"
     gene            135..210
                     /locus_tag="ACHLCY_005434"
     tRNA            135..210
                     /locus_tag="ACHLCY_005434"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:168..170,aa:Ile,seq:cat)
     gene            220..296
                     /locus_tag="ACHLCY_005435"
     tRNA            220..296
                     /locus_tag="ACHLCY_005435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:254..256,aa:Arg,seq:tcg)
     gene            310..386
                     /locus_tag="ACHLCY_005436"
     tRNA            310..386
                     /locus_tag="ACHLCY_005436"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:344..346,aa:Arg,seq:tct)
BASE COUNT          208 a          218 c          232 g          241 t
ORIGIN      
        1 gctggggcgt cgtgcaattg gcgttgagct ggagactgaa cgttttgagc agacggtcag
       61 ggaagttcag gatttagtca gtcagaacgg atgatattgc aggattagtt acgtaccgtt
      121 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
      181 tcgctggttc aaatccagca agggccacca tatcacatac cgccattagc tcatcgggat
      241 agagcgtcag ccttcgaagc tggctgtgcg gggttcaagt ccccgatggc ggtccattat
      301 cagcatcatg cgttgttagc tcagccggac agagcaattg ccttctaagc aatcggtcac
      361 tggttcgaat ccagtacaac gcgccacacc acacttatct gccctgactc tcttttgcgg
      421 gctttttatt acaggaaaga caccggacag tgaaatgtta aatgcctcac aattcaggta
      481 gtcgactgtt gcctgacatg ctgagcgttt gttaaaaaaa tcctgcatga tgaatccccc
      541 tgggcggcgg ggcataatga cagatgtttg gttgcgtatt gtataggcaa gttgcggatt
      601 ctgtctggtc attgcagaat tcaccgggag gcacccggca tcatgctgta tacagagatt
      661 aggcatatat ccaggctcct catcgcagga gcctttttac atgcaaaaaa agcccgagta
      721 ggttcgggca acagcatgag atacttgcat tgtcattttt atcgtgtgga ttttaaccag
      781 ggtttatcag gctgcgcaac tgcgtggcct tttttcattt cttgggctgt agtccccgtg
      841 tgtcattcag gcttccggac tacagcccac tccatatctg atttaataca ctatcccgg
//
LOCUS       DAZGTR010000420          876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGTR010000420 DAZGTR010000000
VERSION     DAZGTR010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.424"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>876
                     /locus_tag="ACHLCY_005437"
     CDS             <1..>876
                     /locus_tag="ACHLCY_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HFP0372420.1"
                     /translation="GRLESVRTLAPGLDIRIPYATDPAGNRLPDPELHPDSTLTAWPD
                     NRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQHG
                     EPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTT
                     RIQTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGLLD
                     RLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALI
                     SEDGNTAWSAEYDEWG"
BASE COUNT          198 a          247 c          308 g          123 t
ORIGIN      
        1 gggcaggctg gagagcgtgc gcaccctcgc accgggcctg gacatccgca tcccgtatgc
       61 caccgacccg gcgggaaacc gcctgccgga cccggagcta cacccggaca gcacgctcac
      121 ggcgtggccg gataaccgca tcgcagagga tgcgcactat gtttaccact atgatgaata
      181 cggcagactg acggagaaga cggaccgcat cccgacgggt gtgatacgga cggacgacga
      241 gcggacccac cactaccact acgacagcca gcaccgcctg gtgttccaca cgcggataca
      301 gcatggcgag ccactggtcg agagccgcta cctctacgac ccgctgggac ggcgaatggc
      361 gaaacgggta tggcggcggg agcgtgacct gacggggtgg atgtcgctgt cgcgtaaacc
      421 ggaggagaca tggtacggct gggacggcga caggctgacg acggtacaga ccgacaccac
      481 gcgtatccag acggtatacc agccggggag cttcgcgccg ctcatccgca tcgaaacaga
      541 caacggtgaa cgggagaaag cgcagtgccg cagcctggcg gagaagatac agcaggaagg
      601 gagcgaagac ggtcacggtg tggtgttccc ggctgaactg gtggggctgc tggacaggct
      661 ggagggggaa atccgggcaa actgcgtgag cagtgaaagc cgccagtggc ttgcgcagtg
      721 cgggctgacg gtggagcggc tggcggcaca gatagagccg gtttatttgc ctgagcgaaa
      781 aatacatctt tatcactgtg accaccgggg cctgccgctg gcgcttatca gtgaagacgg
      841 caatacggcg tggagcgcgg aatatgatga atgggg
//
LOCUS       DAZGTR010000421          867 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGTR010000421 DAZGTR010000000
VERSION     DAZGTR010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.425"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..408
                     /locus_tag="ACHLCY_005438"
     CDS             <1..408
                     /locus_tag="ACHLCY_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372421.1"
                     /translation="DRGEQGKTGLTGDAGPQGPKGDTGTAGPAGPQGPKGETGAAGPV
                     GATGPQGPKGDPGETQIRFRLGPGNIIETNSNGWFPDTDGALITGLTFLDPKDATRVQ
                     GFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
     gene            410..679
                     /locus_tag="ACHLCY_005439"
     CDS             410..679
                     /locus_tag="ACHLCY_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372422.1"
                     /translation="MNILKKLMQRLCGCGKHDDRENGELLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          234 a          174 c          290 g          169 t
ORIGIN      
        1 gacaggggag agcagggaaa gaccggtctg acgggagatg caggtccaca gggtccaaag
       61 ggagataccg gtacggcagg cccggcaggc ccacagggac cgaaaggaga aacaggtgcg
      121 gctggcccgg taggggcaac cggacctcag ggaccaaagg gcgacccggg ggagacgcaa
      181 atacggttcc gtctggggcc gggaaacatt attgagacaa acagcaatgg ctggttcccg
      241 gatacagatg gtgcgctcat caccggactg acctttcttg accccaaaga tgccacacgg
      301 gttcaggggt tttttcagca tttgcaggtc aggtttggtg acgggccatg gcaggatgtt
      361 aagggactgg atgaagtggg cagtgataca ggcagaacag gagaatgaca tgaacatact
      421 aaaaaaactt atgcagcgtc tgtgcggttg cggaaagcat gatgaccgtg aaaacgggga
      481 gttacttaca gcacagctgc gactgggacc ggcagacatt ctggagtcag atgagaatgg
      541 cattatcccg gagcaggcca gggtaatcac gcaggtggtg atactggatg cggataaaaa
      601 gcagatacag tgcgtggtaa gaccgctgca aattctgcgt gctgacggga cgtgggaaaa
      661 tattggcggg atgaaatagt cgacagcttc acaaaaaccg gagtccggct ccggtttttg
      721 ttgtcatgtc cggtggatgt ttgttatgac tccctgtgtt tggaatgaat atttaataac
      781 aggtgtctgg aaatataggg gcaaatctac tggataggct attggggcgt gagaatcgaa
      841 tggagagaag ggctgtggct ctggaaa
//
LOCUS       DAZGTR010000422          863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGTR010000422 DAZGTR010000000
VERSION     DAZGTR010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.426"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            54..290
                     /gene="ycgZ"
                     /locus_tag="ACHLCY_005440"
     CDS             54..290
                     /gene="ycgZ"
                     /locus_tag="ACHLCY_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP0372423.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            333..605
                     /locus_tag="ACHLCY_005441"
     CDS             333..605
                     /locus_tag="ACHLCY_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP0372424.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            634..>863
                     /gene="ariR"
                     /locus_tag="ACHLCY_005442"
                     /gene_synonym="ymgB"
     CDS             634..>863
                     /gene="ariR"
                     /locus_tag="ACHLCY_005442"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP0372425.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTL"
BASE COUNT          295 a          143 c          180 g          245 t
ORIGIN      
        1 ttgctattgt tacctcactt aacattgatt aacattttta acagaggcgt agcatgcatc
       61 aaaattcagt gactttagat tctgcgggag caatcactcg ttactttgca aaagctaact
      121 tgcctactca gcaggaaact ctcggggaaa ttgtgactga gattttgaaa gatggacgta
      181 atctgagtcg aaagtcgctt tgtgccaaac ttctttgccg actggaacac gcgactgggg
      241 aagaggaaca gaaacattat aatgcactaa ttgggttgct ttttgaataa tgcaacagcc
      301 tgattattta acaggttagc tatgaagtcg ttatgaagac atctgataat gaacgtataa
      361 aatatgaaat tactggccag gcggtgatcc agatactgcg catgaagata aatttttcat
      421 tgcagactct tattaagcaa ttactcgtaa tgaaatcggc tgaggaagat gctttccgac
      481 gcgatttaat tgacagcata attcgtgatt ttagcaatag tgattcagga gggccaaacc
      541 gaagaacagc gacagctgac aataaaagta tgttcaatgg taagaaaata aacagaatac
      601 attaaaattt cataagtaag atgagaggtt accatgcttg aagatactac aattcataat
      661 gcaataactg ataaagcgtt agcaagttac tttcgcagtt cgggtaattt gttagaagaa
      721 gagtcagcag tgttagggca ggctgtcacc aatttaatgc tttcaggcga taatgttaat
      781 aataaaaata ttatcttaag tctgatacac tccctggaaa caacaagtga tattctcaaa
      841 gctgatgtga ttagaaaaac act
//
LOCUS       DAZGTR010000423          856 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGTR010000423 DAZGTR010000000
VERSION     DAZGTR010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.427"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..259
                     /locus_tag="ACHLCY_005443"
     CDS             <1..259
                     /locus_tag="ACHLCY_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0372426.1"
                     /translation="AVYADYINNTTDFRPKPERIFICGETLKCEDPSIYIRQKYGHHG
                     VGVDQNQQFDNGVMGLNSTKKVLDKNNKIIYSSGLSCFQSH"
     gene            521..>856
                     /locus_tag="ACHLCY_005444"
     CDS             521..>856
                     /locus_tag="ACHLCY_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372427.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     DNNYQFIVTI"
BASE COUNT          292 a          132 c          166 g          266 t
ORIGIN      
        1 agctgtatat gctgattata ttaataacac tactgatttc agaccaaaac ctgaacgtat
       61 cttcatatgt ggagagacac tcaaatgtga agatcccagc atatacatta gacaaaaata
      121 tggccatcat ggggtcgggg tagatcagaa tcaacaattt gataatggtg ttatggggct
      181 aaattccacg aagaaagtac ttgataaaaa taacaaaata atttattcct cagggcttag
      241 ttgttttcaa tctcattaaa actccatgaa tacggtggta tgccttttta atcctgccag
      301 tatttcatag gttttcttaa tcgattgtag aggtatgtac tgatgatgcc tctacaatct
      361 ttagggactt tctctgctat gagataagaa atttcatatt atgctttaag gctgaatgat
      421 ttgtgcaaaa tataattatt tttccatcga acatgaggtg tatgtcataa gattaaatgt
      481 taactatgca ttcctcgcgg tgaattatat gaggtaaaag atgaaaattg gaactgtggc
      541 aggcactaac gacagtacaa cgacaatcgc aacaaatgat atggtgcaag agcatgttac
      601 taacttcact aaagaactgt ttgggtatat tgcaaatggt ataggtgatg atatatcaag
      661 tattgccagg acaatgctcg gtgaagttgt ggaaaaaatt gatgattggc aaatagaacg
      721 tttccaacaa tctattcgag atgacaaaat ttcatttacc atacaaacga atcattcaga
      781 aaaatatagt atgctatcgg gcatgcgcgc tcatattcta cgtagggata ataattatca
      841 gtttattgtg acaatt
//
LOCUS       DAZGTR010000424          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGTR010000424 DAZGTR010000000
VERSION     DAZGTR010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.428"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            47..785
                     /locus_tag="ACHLCY_005445"
                     /pseudo
     CDS             47..785
                     /locus_tag="ACHLCY_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          282 a          174 c          176 g          211 t
ORIGIN      
        1 tagctaaaca ttaccatgat tatgacaggc gagggagtgt ccaattatga attcaaaaaa
       61 gctttgttgc atatgtgtgt tattctcgct gcttgcagga tgtgcctctg aatcttctat
      121 tgatgaaaag aagaaaaaag ctcaagtcac acaaagtaat attaataaaa acactcccca
      181 gcaactgaca gacaaagatt tattcggtaa tgaaaccact ctggccgtat ccgaagagga
      241 tattcaagct gcgcttgatg gagatgagtt ccgcgttccc cttaattctc ctgtaatact
      301 tgttcaatcc ggcaaccgcg caccggaaac cattatgcag gaggagatgc gaaaatatta
      361 tactgtttcc acattctctg gtatcccgga caggcaaaag cctctgactt gtaacaaaaa
      421 caaagataaa aatgagaacg aggatgttgc cagtgctgag aatatgaact ggatgcaggc
      481 actgcgtttt gtggctgcaa aagggcatca gaaagcgatt attgttttcc aggatacgtt
      541 gcagacagga aaatatgact ctgcgctgaa atcaacagta tggtccgact ataaaaatga
      601 aaaactcacc gacgctatct ccctgcgcta cctggtcgcc ggtgaattat gaatataaag
      661 tgcttccacc attgcccgac aagaatgaag ccagtacgac tgatatgaca gagcagcaaa
      721 tcatgcaact taagcaaaag acctataaag cgatggtaaa agatttggtt aaccgctatc
      781 aataataaat tatatctgcc gccaggaatt tctggcggca ataatacaaa attctttgca
      841 taa
//
LOCUS       DAZGTR010000425          843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGTR010000425 DAZGTR010000000
VERSION     DAZGTR010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.429"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>843
                     /locus_tag="ACHLCY_005446"
     CDS             <1..>843
                     /locus_tag="ACHLCY_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP0372428.1"
                     /translation="SNKIGLRVYTVVSEMSIEAWITKEPQPFVYRRSGAYQKLTIGSD
                     WGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVP
                     PDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYY
                     DVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDNTLITFSNEEI
                     IKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRTFATALTNIEK
                     YPDY"
BASE COUNT          265 a          156 c          179 g          243 t
ORIGIN      
        1 tgtcgaataa aataggtctg cgtgtttata ctgttgtctc tgaaatgtcg attgaagcat
       61 ggataacaaa agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      121 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      181 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      241 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      301 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      361 acgaggtcga gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg
      421 gagggattat tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt
      481 atgatgttga agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      541 ctaaaggtat tatcccagaa aaaattatca ccaaaagaag cgagcgtact gacgaaatca
      601 taagaattct cgaatatatt gataatacgt taattacctt tagcaatgaa gaaataatta
      661 aatgtcaaat tgcaatccaa tccataatta gtaaaaacaa taatccttca gagttcagga
      721 ttatggctac tggtcatgcc catttagata tagcgtggat gtggccttta agagaaggac
      781 gcagaaaagc aataagaaca tttgccaccg ccctgacaaa cattgaaaaa tatcctgatt
      841 aca
//
LOCUS       DAZGTR010000426          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGTR010000426 DAZGTR010000000
VERSION     DAZGTR010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.430"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..423)
                     /locus_tag="ACHLCY_005447"
                     /pseudo
     CDS             complement(1..423)
                     /locus_tag="ACHLCY_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000018100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
     gene            complement(424..597)
                     /locus_tag="ACHLCY_005448"
     CDS             complement(424..597)
                     /locus_tag="ACHLCY_005448"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372429.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
BASE COUNT          199 a          225 c          147 g          263 t
ORIGIN      
        1 gacaaaaaac caagcatggt aataaaccga actccccaca aacttctgtt gcctcagata
       61 ctcccccccc tttcctcagg cacaaatgtt gttccataac gaaccagccc tgttccttta
      121 aactctgcat caatggcata atgtatactc accagactga gtttgctcct gcatcattat
      181 ttcgatacgt taatgcattg ttcattgcat ccggcattcc ccatcgaata tttactttgt
      241 tatatcaggc agcccataac caggtaaatt tttaaggctt actgactgaa tattcaaaat
      301 gtgttacatg ttatcaaacc tttaaagaat aacctgctct tcttcattgg ccggatttag
      361 ctcaatcttg tcagcaaaac taatataaag ccgttcataa agagaaggat gtagcttcgt
      421 tatttatctt ttacatgtaa cagtgccagg ggatattccg cacatctctg tggctatcat
      481 ccccaccacg tctccagtgg cagcacctgc ggcactggca cgggcgttat ttccctgtac
      541 caccgccagt gcagcattca ccacggcatg ggctgccaca tttgcagtct tattcaccac
      601 acctgtaacc tgtctcttat acacaaatcc ccacccgggg atttgtgatg tctgtcagtc
      661 tgctttaggg ggactctttc catccggtag tttccttcct ccccggcagc gattttttga
      721 actttttctt cacccgttga tcttctctta tatttcattt tcttcgggtc tgttatggta
      781 ctttctgatt gttattcact ggctaacgag cagtctggac atgcccgtct cggt
//
LOCUS       DAZGTR010000427          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGTR010000427 DAZGTR010000000
VERSION     DAZGTR010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.431"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..90
                     /gene="yjbL"
                     /locus_tag="ACHLCY_005449"
     CDS             <1..90
                     /gene="yjbL"
                     /locus_tag="ACHLCY_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFP0372430.1"
                     /translation="HRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            114..821
                     /gene="yjbM"
                     /locus_tag="ACHLCY_005450"
     CDS             114..821
                     /gene="yjbM"
                     /locus_tag="ACHLCY_005450"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP0372431.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVENKNDLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
BASE COUNT          275 a          124 c          167 g          261 t
ORIGIN      
        1 catcgggata taattattgc aaaaatagat ttgtttattc aacgaatcac gcataattta
       61 tggcatgcac gtaaaaaaat gtgtttttaa ttgagcaaat aaatgatctc aaaatgtggg
      121 tcaataagta tattgacgat tgcactgatg aggatttaaa cgatcgtgac tttattgcat
      181 cagttgttga ccgggctatt tttcatttcg cgattaatag tatatgtaat cctggggata
      241 ataaagatgc aatgcccatt gaacaatgta cttttgatgt agaaaataag aatgaccttc
      301 cctccacggt tcagctattt tatgaggaat ctaaggataa tgaaccttta gcgaatatac
      361 attttcaagc aataggttct ggttttttaa cgtttgttaa tgcctgccag gaacatgatg
      421 acaacagctt aaaattattt gcttcgctgt taatttcact ttcatattct agtgcctacg
      481 cagatttatc agaaacagtg tatattaatg aaaataatga gagctacctg aaagctcagt
      541 ttgaaaaatt atctcaacgt gatatgaaga agtacctggg agagatgaag cgtctggctg
      601 atgggggaga aatgaatttt gatggctatc tggataagat gtcacatctg gtgaatgaag
      661 gaacgctcga tcctgatatt ttaagcaaaa tgcgagatgc tgcaccacaa ttaattagct
      721 tcgcgaagtc gtttgaccca acctcaaagg aagagattaa aatacttaca gacacttcta
      781 aattaattta tgatttgttc ggggttaaat cggagaaata atatgtg
//
LOCUS       DAZGTR010000428          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGTR010000428 DAZGTR010000000
VERSION     DAZGTR010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.432"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..274
                     /gene="escR"
                     /locus_tag="ACHLCY_005451"
     CDS             <1..274
                     /gene="escR"
                     /locus_tag="ACHLCY_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFP0372432.1"
                     /translation="KKLGNETILKKDSLFILLPAFTMGQLEAAFKIGFLLYLPFIAID
                     LIISNILLALGMMMVSPVTISIPFKILLFILVGGWQKLFEFLLVVN"
     gene            274..543
                     /gene="escS"
                     /locus_tag="ACHLCY_005452"
     CDS             274..543
                     /gene="escS"
                     /locus_tag="ACHLCY_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFP0372433.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            543..>824
                     /gene="escT"
                     /locus_tag="ACHLCY_005453"
     CDS             543..>824
                     /gene="escT"
                     /locus_tag="ACHLCY_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFP0372434.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAID"
BASE COUNT          229 a          137 c          157 g          301 t
ORIGIN      
        1 aaaaaaattg ggtaatgaaa ctatcttaaa aaaagactct ctatttatac tgttaccggc
       61 tttcacgatg gggcagcttg aagctgcatt caagataggt tttttgctct atttaccctt
      121 tattgcgata gatttgatca tttccaatat cttattggcc ttgggtatga tgatggtatc
      181 gccagtaaca atttcgattc cttttaagat cttgttattc attcttgttg gaggctggca
      241 aaaattattc gaatttctgt tggtggtgaa ttgatggata ctggatattt tgttcaatta
      301 tgtgtgcaaa cgttctggat aatatttatc ctctcattgc ctacagtcat agcggcttct
      361 gttatcggta ttattattag tctggtccag gctataacgc agttacagga tcaaacattg
      421 ccttttttgc taaaaataat agcagtgttt gctacgcttg ccctgactta tcactggatg
      481 ggaacaacaa tcatcaactt ctcgtcgata atatttgaaa tgattccgaa ggtgaacggc
      541 taatgaatga gataatgacg gtcatagtat catcatttta ttgtatactc aggccgctgg
      601 ggatgtttat tatcctacca atattttcaa caggtgtttt actcagtaac tttattcgca
      661 actcaattat gattgcgttt acgcttccga tcatagtaga gaactataca ttttcggaaa
      721 aattgccatc tggaatcttt cagttgacag gaatagcgct aaaagaaata agtattggtt
      781 ttttcattgg gttatcattt actattcttt tttgggcaat agat
//
LOCUS       DAZGTR010000429          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGTR010000429 DAZGTR010000000
VERSION     DAZGTR010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.433"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..257)
                     /locus_tag="ACHLCY_005454"
     CDS             complement(<1..257)
                     /locus_tag="ACHLCY_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
                     /protein_id="HFP0372435.1"
                     /translation="MPFSIKSIFSGHTWHQPEISRPIADKSSTKNCILDSTTCNVDGF
                     TVFNRRSCSFDMRPPGSADRTPQLRLSISEVAWMSKIIETET"
     gene            685..>822
                     /locus_tag="ACHLCY_005455"
     CDS             685..>822
                     /locus_tag="ACHLCY_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372436.1"
                     /translation="MIFIASSSREQTTASDGKMGTTGYSGNLAEEAYGTDCNVKYKTL
                     KI"
BASE COUNT          232 a          155 c          167 g          268 t
ORIGIN      
        1 gtctctgtct ctattatttt tgacatccat gcgacttctg atattgataa tctcagttgt
       61 ggagttctgt ctgcgcttcc gggaggacgc atatcaaaac tacaggatct tctgttaaac
      121 actgtaaagc catctacatt gcatgttgtt gagtctaaga tacaattttt agtagatgat
      181 ttatctgcta taggcctaga tatttcaggt tgatgccaag tgtgtcctga aaaaatactt
      241 tttattgaaa atggcatttt tatcctcaca ttatgtcttt ctcttatagt gcaatttaat
      301 gtatttagaa acattaaact atgaatctaa aaaggctacg cagagtactg ttacacttta
      361 acaaatcata tttaccactc tggaaatggg atggctggtt cataaaataa tgagtctttt
      421 tcaacggttg ttacattggt gtactgatga ttatgccttg tcgctaagag ctaccataac
      481 cgtacagcgt gattcatcca tggcgaataa atcgactgat aaatccccct gagtttcagc
      541 tcttgctgtt ggaccttgcc cgtttcgcta tggaacgcgt accagctaga gaatattatt
      601 cgttctcctc caatgttacg caagttttgt aaacggtaac caatcgtgat tagtcctgca
      661 tgtaacggac atcaagctgt ctggttgatt tttattgcca gcagttcgag agagcagaca
      721 actgcgtccg atggaaaaat gggcacgaca gggtattcag gcaacctcgc tgaggaggct
      781 tatggtacag attgtaatgt gaagtataaa acgttaaaaa tt
//
LOCUS       DAZGTR010000430          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGTR010000430 DAZGTR010000000
VERSION     DAZGTR010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.434"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..578)
                     /gene="fimE"
                     /locus_tag="ACHLCY_005456"
     CDS             complement(<1..578)
                     /gene="fimE"
                     /locus_tag="ACHLCY_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP0372437.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEK"
BASE COUNT          259 a          191 c          154 g          215 t
ORIGIN      
        1 aatttttcgt ttatgagatt atttctttcc cataatccgg caaaacgagc agcattactg
       61 gcggtataac gcacagtatg gcgaatattt cgatgcccga gataatcctg aattaaacga
      121 gtatctgcac cacgctccgc cagttcataa ccgcaagcat gccttaacat atgaggatga
      181 gtctgcgtta cggttccagc ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg
      241 cgagaaagcc gactcccgcg gcgagaaata aatatagcgt cagtccggtc agcgcctttc
      301 cagttagcac gttcctgggt ccatcgttcc acggcttcac gctcatcaaa acgtaacggg
      361 tgaacggtag aaaatccgtt cttcagtcgg cgaatattta ttctaccttc attaaggtca
      421 aggtcctgat aatgcagatc aagcagttca ctaatacgca tcccatgccg atatgccaac
      481 agaataagac aataatctct ggctcccgtt gccccgtaac aaaccgcctg catcatggcc
      541 tgaacttctt taccggtaag ataacgacgt ttactcacaa tagtagtact cctaactgag
      601 atatatttga atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa
      661 aaagataacc acacacggag tgatgtggct atcagtcaat tacattaaat atcatacaaa
      721 aataaaatat cactgatgtg acaacatcta ttttcattga tttgattcaa tataacaata
      781 gattgcagtg gattaacttc cagttgctta aaaaataaa
//
LOCUS       DAZGTR010000431          817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGTR010000431 DAZGTR010000000
VERSION     DAZGTR010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.435"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..817
                     /locus_tag="ACHLCY_005457"
                     /pseudo
     CDS             1..817
                     /locus_tag="ACHLCY_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491406.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
BASE COUNT          270 a          156 c          161 g          230 t
ORIGIN      
        1 aaaagccgtt atgaattaca tggaatatct ggtaacttgt cagttggatg aacaacaaat
       61 gtcatcactg ctttatgaaa gagatgattt aagcgccatt gatttttcat tgctcatgaa
      121 aacgataaaa gccttttcat ttggtggaga tcttcaaacc cttgcatcaa aaccgggttc
      181 cacaatctca agtatcccat cagaaagacg gatattgatt aacattaacc acgattttcc
      241 taacaatggt aatcttttca atgactttct atttaaccat caacaagatg aacaattagc
      301 aatggcatat atagccgctc tgccgttcag caggccttta gtttactggg atggccaagt
      361 attaaaatca acgactgaaa ttaaaaatta tgatgggtcc acgcgtgtcg gcggtgaggc
      421 gtagcttaat aaaggttgct ctacctatca gcagctctac aatgaattcc acgcattata
      481 tatagataaa gcaggaatat ggagcgcatt tgagggtgta tctgcaacta aaaacgttct
      541 ggcctttagt cgtggggatt ctgtgaacat taatcactct cctcatgatg gactagttat
      601 aataaataaa ggaaacgaag aagttgaagg tacctggcct aacaaattgc aacctggaat
      661 atacaaaaac atggggagta atagcgttaa cattattatt aataataccc gaaaaattat
      721 cccccctggt aaagtattta cgcttagagg cggaactcta aatatcaata ttcctgggcg
      781 tagcgctctt cttttaggga aaacaggaga accgccg
//
LOCUS       DAZGTR010000432          812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGTR010000432 DAZGTR010000000
VERSION     DAZGTR010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.436"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..723
                     /gene="ydeO"
                     /locus_tag="ACHLCY_005458"
     CDS             <1..723
                     /gene="ydeO"
                     /locus_tag="ACHLCY_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP0372438.1"
                     /translation="FNANILDKDYALSDGEILMVDNAVRTHFEPYERHFKEIGFTENT
                     IKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCL
                     SIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQ
                     EQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVS
                     KEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          245 a          165 c          166 g          236 t
ORIGIN      
        1 ttcaacgcta acattttaga taaagattac gccctctctg acggcgagat cctgatggta
       61 gataacgctg ttcgtacgca ttttgaacct tatgagcggc attttaaaga gatcggattt
      121 actgaaaata ccattaaaaa atatctacaa tgcactaaca tccagacagt gacggtgcct
      181 gttcctgcga agtttttacg tgcttcaaat gtaccgactg gattgcttaa tgaaatgatt
      241 gcttatctca actcggaaga acgcaatcat cataattttt cagaactttt gcttttttct
      301 tgcctgtcta tttttgccgc atgcaaaggt ttcattacac tattaactaa cggtgtgcta
      361 tccgtttccg gaaaagtgag aaatattgtc aacatgaagc tggcgcaccc atggaagctg
      421 aaagatattt gtgactgcct gtacatcagt gaaagcctgt tgaagaaaaa actaaagcaa
      481 gagcaaacga cattctcaca gattctatta gatgccagaa tgcagcacgc aaaaaatttg
      541 atacgcgtag aaggttcagt caataaaatt gccgaacaat gtggttatgc cagtacatct
      601 tattttattt atgcgttccg caaacatttc ggcaacagtc cgaagagagt ttctaaggag
      661 taccgttgtc aaagtcacac gggtatgaat acgggcaaca cgatgagtgc tttagctatt
      721 tgattatttg ctaacgagta gtcaaccacg cacgctacgt aagaattata tggggcagcc
      781 attccctgcc ccgcgttgtt tttaggcgat at
//
LOCUS       DAZGTR010000433          804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGTR010000433 DAZGTR010000000
VERSION     DAZGTR010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.437"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..709)
                     /locus_tag="ACHLCY_005459"
     CDS             complement(<1..709)
                     /locus_tag="ACHLCY_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFP0372439.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLA"
BASE COUNT          323 a          174 c          110 g          197 t
ORIGIN      
        1 aagctaaaaa tgcgagaatg gcatatttta tacaatacgt agtatctaat ttcaccttga
       61 aacgatgtat accgaaactg gtattgctcc agagaaaact cattaccagg ctatataaag
      121 taccattaaa aataccaatg gaaacaatac caactattgc catcagaacc acagatataa
      181 taacactact tgaactattg gcatgtagca tctttgtcga gataaagaaa acagtcccca
      241 tcccaatggc cattaaaatc ggcaagaaaa aggttaccca ccagaaccct ttcatagagc
      301 aattaaaact aaatcttacg ccgttgagac tggtcatcaa ggcctgataa cgcagtcctt
      361 ttgctgccat aaatataagc aaaaccaaca gtaacaaagt caaaacacag ccaacaagtg
      421 gcatatctgc tgacactgtc attaagattg cgaaatagca aaaaacaaaa acaagacaac
      481 taacaaaaac attcccacca gtaattccat aagaaaatcg ttgtccgtta acttccatat
      541 tagcatagag ataacgctta catttcatta atgcccatgg caaatagata cctagcgtta
      601 taatcgttaa caacacattc accacacaaa taagaaaata tctctcacct gtaccatgaa
      661 aaacaaacga atgggacgga acatccattt cattaataac ttgagccata aaaattccct
      721 gaattaaaaa atccattgat ataatttata tatacagaca gtaaaatgtt ctgccacgtt
      781 acaatactct tatcagtaat aggc
//
LOCUS       DAZGTR010000434          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGTR010000434 DAZGTR010000000
VERSION     DAZGTR010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.438"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..395)
                     /locus_tag="ACHLCY_005460"
                     /pseudo
     CDS             complement(1..395)
                     /locus_tag="ACHLCY_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(656..>799)
                     /locus_tag="ACHLCY_005461"
     CDS             complement(656..>799)
                     /locus_tag="ACHLCY_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP0372440.1"
                     /translation="NGIIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIG
                     GMK"
BASE COUNT          203 a          211 c          180 g          205 t
ORIGIN      
        1 ccgtatcctg tcatacgcgt aaaacagcca gcgctgacgt gatttagcac cgacgtagcc
       61 ccactgttcg tccatttcag cgcagacaat cacatcactg cccggttgta tgcgcgaggt
      121 taccgactgc ggcctgagtt ttttaagtga cgtaaaaccg tgttgaggcc aacgcccata
      181 atgcgtgcac tggcgcgaca tccgacgcca ttcatggcca tatcaatgat tttctggtgc
      241 gtaccgggct gagaggcggt gtaagtgaac tgtagttgcc atgttttacg gcaaggagag
      301 cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg
      361 gagcaggaag gacatctgat ggaaatggaa gccacgcaag caccttaaaa tcaccatcat
      421 acactaaatc agtaagttgg cagcattacc gagattgttt tgtcggaact gatacgctgg
      481 cgctaatagg tagtagggtt cttatgctaa acatacaacc tctttcttaa ttctcgaacc
      541 tgtttaggat tctgtgtaaa ttcaaaataa acctgtctga acattcctaa caaacatcca
      601 ccggacatga caacaaaaaa ccggagccgg actccggttt ttgtgaagct gtcggctatt
      661 tcattccgcc aatattttcc cacctcccgt cagcacgcag aatttgcagc ggtcttacca
      721 cgcactgtat ctgcttttta tccgcatcca gtatcaccac ctgcgtgatt accctgtcct
      781 gctccggaat aataccatt
//
LOCUS       DAZGTR010000435          797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGTR010000435 DAZGTR010000000
VERSION     DAZGTR010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.439"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            125..>797
                     /locus_tag="ACHLCY_005462"
     CDS             125..>797
                     /locus_tag="ACHLCY_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0372441.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKVIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALED"
BASE COUNT          260 a          134 c          170 g          233 t
ORIGIN      
        1 tatggcttta gaatattttt atttctaata gacgagattt ttcctgttat gatataatat
       61 gccgaattaa cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat
      121 tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac
      181 gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct gctgctaaac aaggtgaacc
      241 cgatccagaa ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt
      301 tccaaagcac gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt
      361 cgtgcaggga cgttatgaag gctttggtcc taatggctca atgattatcg ccgagacatt
      421 gacttcaaat gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg
      481 caatatcgga gcggcaggtt ctgtcagcta tatgtttgac aatacgggtg tgattgtatt
      541 taaaggatca gaccctgacc atatttttga aattttactt gaagctgaag ttgatgttcg
      601 tgatgtgact gaagaagaag gtaacattgt tatttatacc gaacctactg accttcataa
      661 agtaatcgcg gctctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat
      721 gattgctcaa tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt
      781 tgatgccctt gaagatg
//
LOCUS       DAZGTR010000436          796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGTR010000436 DAZGTR010000000
VERSION     DAZGTR010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.440"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..456)
                     /locus_tag="ACHLCY_005463"
     CDS             complement(<1..456)
                     /locus_tag="ACHLCY_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372442.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLITELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKT"
     gene            581..796
                     /locus_tag="ACHLCY_005464"
                     /pseudo
     CDS             581..796
                     /locus_tag="ACHLCY_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          251 a          166 c          130 g          249 t
ORIGIN      
        1 agttttatca agcttagttt tcagcctttt aacagcctcc aaggaagcat caagaactgg
       61 tgtctgattc ttcaaagctt ctgaaaaact tttttgtgtt gattttcgag cttgttgttg
      121 agcagatttt cttaactctt tttcacgctt aacttgttct aaaagctctt tacggtcatc
      181 agaacgtgaa ccaaaaaatc tatcagcaag ttcagttatc aaattagata taatagtttt
      241 cagttctctt gcagcctggt ttcttatgaa tccctctctc cccgacttat ctttcagctc
      301 tttattactg gattgagtaa ttccaatata accaaaaatc cttctattag accaataata
      361 tcgccctgca ttccatgaac gtctttcttc tatctggaag aaatcattat ctactcgacc
      421 ataaggtaat actctcaagc tatccctaaa aatcattagt cctgcatact ttttggcctt
      481 aagatcaaag tgggaatgtt cacgttcagt atgtgatgta ttttgtgaaa ggaattcaaa
      541 cgttcctatc tgaagctcaa atgggccgac ccctgcgcgc atctctaagg atgaatggga
      601 aaacctccat gcattaaaag aaaagcgttt aaatggaatg cctaaaacaa cacccaaaag
      661 tgaccaggtt attatgttgc aaaatcaata cgttcgtgaa atgcgaaaat atggcgtacg
      721 aggtttacgt tatgatgcgg caaaacactc aaaacatgaa caaatagaaa gatcaataac
      781 cccaccgctt aaaaat
//
LOCUS       DAZGTR010000437          793 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGTR010000437 DAZGTR010000000
VERSION     DAZGTR010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.441"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            44..691
                     /gene="nleG"
                     /locus_tag="ACHLCY_005465"
     CDS             44..691
                     /gene="nleG"
                     /locus_tag="ACHLCY_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase NleG"
                     /protein_id="HFP0372443.1"
                     /translation="MPVILNFSSERVLSESELEALRHVGRVSQSEQLVVRGRTMRLHH
                     ISFMDSFSVEPVSGGLLDRLSARGHRLLAENLEIQLNRGHTFLQAFRLYMEQSRATPC
                     TRQNVSSAIQNKINSHAFTVSHQDFSCHEQHLNCPITLCIPETGVFVRNAKNSEICSL
                     YDHNALTELIRRNAPHPLSREPFVPEMIVSKDECHFNLIEQYFCILATQNICTRI"
BASE COUNT          247 a          162 c          149 g          235 t
ORIGIN      
        1 tcaccaatat ggttcaataa catagactaa agagggagaa aatatgcctg tcatattaaa
       61 cttttcgagt gaaagagtat tgtctgaaag tgaactggag gctttacgtc atgtcggaag
      121 agttagtcag agtgaacagc ttgttgtgag aggccgcact atgagacttc atcatatttc
      181 atttatggac agttttagcg tggaaccggt ttccggcgga ctgttggatc gccttagtgc
      241 aagagggcat cgccttctcg cagaaaatct ggagatacag cttaacagag gccatacttt
      301 tttgcaggca ttccgtttat atatggaaca gagccgtgca accccatgta cgcgccaaaa
      361 cgtcagtagt gcaatacaaa acaaaattaa ctctcatgca tttactgtta gtcatcaaga
      421 cttttcctgt catgaacaac acctgaattg cccgataaca ttatgcattc ctgaaactgg
      481 tgtttttgtc agaaatgcaa aaaactcaga aatatgttca ttatatgacc acaatgcact
      541 cactgaactt atccgacgca acgctccaca ccctctcagc cgtgaaccct ttgttccgga
      601 aatgattgtc agcaaagatg aatgccattt taatctaata gaacaatatt tttgcatatt
      661 agccacacaa aatatatgca ctagaatttg actgtatggt tcgatttaaa tgttgcaaaa
      721 ttaattttac tagttattgc catctatttc atactcccta attcttgata caatctgtct
      781 gtcaaaatag tag
//
LOCUS       DAZGTR010000438          781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGTR010000438 DAZGTR010000000
VERSION     DAZGTR010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.442"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..160)
                     /gene="ydcD"
                     /locus_tag="ACHLCY_005466"
     CDS             complement(<1..160)
                     /gene="ydcD"
                     /locus_tag="ACHLCY_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
                     /protein_id="HFP0372444.1"
                     /translation="MLQIIRGKLVIFLITLCLFVVYLGFDNNSNSDIVFYGHKTPKSV
                     EIYLSEKNI"
     gene            complement(144..>781)
                     /locus_tag="ACHLCY_005467"
     CDS             complement(144..>781)
                     /locus_tag="ACHLCY_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0372445.1"
                     /translation="HQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGW
                     NMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDA
                     IVGGAHNGAADAMRHCYLMCRMTKTFGSIIADVIGKNHEAAGDRQGQPAKERIMDLKN
                     NTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          221 a          191 c          134 g          235 t
ORIGIN      
        1 taatattttt ttcagaaaga tatatctcaa cgctctttgg tgttttatgt ccataaaata
       61 cgatgtcaga atttgaattg ttatcaaacc caaggtaaac aacaaataaa caaagggtaa
      121 ttaaaaaaat gacaagtttg cctctaataa tttgaagcat ctgaactccc ttgctttgct
      181 tttattggat tatctgcttt tataccatct aacccgaaga gttgcccagt gttatatttt
      241 tcaatacatg catcgctaca tttggcagaa aaatcgccac aagcaatacc gacagtgtta
      301 tttttaagat ccatgattct ttctttagct ggctgacctt gtctatcccc tgccgcctca
      361 tgatttttac ctatcacgtc agctattatt gatccaaatg tcttagtcat tcgacacatc
      421 aagtaacaat gtctcattgc atcggcggcc ccattatgag ctccgccaac aatagcatcc
      481 atatcataac cgaactggta agcactgtta gtaatgctgt ttgcagcaat caatccaggt
      541 acaccagata cggcataaat tagtccacct gatgtcatat tctcaatcgc atctaacccc
      601 attgggtcta tcacttgtat gggattcaac ggatactgat acatattcca tccacctctc
      661 aacccaatcg ggtccggggt gatataccgc ccctgcaacg gatcgtagta ccggtgacgg
      721 ttatagtaca gccccgactc cttatcatac tgctgccccg gcagccggta cggctggtgc
      781 a
//
LOCUS       DAZGTR010000439          773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGTR010000439 DAZGTR010000000
VERSION     DAZGTR010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.443"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..71)
                     /locus_tag="ACHLCY_005468"
     CDS             complement(<1..71)
                     /locus_tag="ACHLCY_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372446.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGA"
     gene            complement(68..418)
                     /locus_tag="ACHLCY_005469"
     CDS             complement(68..418)
                     /locus_tag="ACHLCY_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0372447.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(428..754)
                     /locus_tag="ACHLCY_005470"
     CDS             complement(428..754)
                     /locus_tag="ACHLCY_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0372448.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
BASE COUNT          161 a          276 c          175 g          161 t
ORIGIN      
        1 gcaccactca gaagctgcaa atcgcggctg atgtcctcca gccccgaaaa atccagcagg
       61 gtttcgatca tttttcccct cccagccgac agagaatttc cagacgccca ccggtcgcat
      121 ccggcacggg cagcccgaca acgttcagga tccggtcacg ccagggacca ctcagcacat
      181 gaagtcgtga cgctgccgtg atttcccggc cggactgacc gcgcacccag atgcggattt
      241 ccgcctgcgc catttccgca ccggactgca tccgctcccg gctgctcctg ccacggatat
      301 ccgcatgaat tttcccgcat gacacccatt cttccgtcat ttctccggca gcattacggg
      361 ttaacaccgg gttcagaaca cttatcatct gtgtcagacg acctgcagat attgccattc
      421 cccctcctca taacaccgtc ggacaacgca aatcgtaaat cagcacggac acagaaaacg
      481 gcagttcccc ctgcacgagg tcttcccgct cagcaagatc cggattccgg tacagcatcc
      541 cggtcagtcg catggcagcc cccttcatcc gggttaatgc ctcgcccggg atcagctcac
      601 cgtcctcacg aatcacctta tcccggctgc cctgaatgta ggccagcagc acggcggtag
      661 cctgacgaac cttgtccatc agcatgtcat catccgcgtc atggtcgaca cgcagatgtg
      721 ccttgatctc ttccagtgtc agtaatgccg tcattttccg cctcctgcat ccc
//
LOCUS       DAZGTR010000440          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGTR010000440 DAZGTR010000000
VERSION     DAZGTR010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.444"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(61..>768)
                     /locus_tag="ACHLCY_005471"
     CDS             complement(61..>768)
                     /locus_tag="ACHLCY_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP0372449.1"
                     /translation="ITLLRHISTINGFDNLMAQPLLSDEPLTALMDHYLDTDALADGL
                     PLYVSLYPTEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLL
                     FRPREVQGTMFGDGGMGGWRNMQGNTPVTHLSDGSLWDRQAFPDTTILEIRPRKRLKY
                     AGDGGNSGGLLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLD
                     ITEEADLALRNAMARIK"
BASE COUNT          173 a          224 c          191 g          180 t
ORIGIN      
        1 ttcaataaca ccagacagga aaaacaccac cacaggcggt ggcgtttctt tggggcgtct
       61 ttatttaatc cgggccatcg cgtttctcag tgccaaatct gcctcttccg ttatatccag
      121 gcttttctgc aataccgcct ctgaccgggt cagtgcctga cgtgctgcca gcggtttccg
      181 gatatgctcc atcgccagca tcgtgtcttc atagccctgc tgacgccagg cgtcggtatg
      241 tgccgatgta aaactgagca gaccgccgct gttgccacca tcacctgcat atttcagcct
      301 tttccgggga cggatctcaa ggattgtggt gtccggaaaa gcctggcgat cccataaaga
      361 accgtcactc agatgtgtca caggggtatt tccctgcata tttcgccatc ctcccatacc
      421 accatcaccg aacattgtcc cctgaacctc acggggacgg aacagcaggg gcagcgcggc
      481 tgacgcaagc agagcctctt tctgctgtcc gcggggcagg ctctggatat gctgaaaaac
      541 ggcgtttttc gtggttccga cacccagttc agcacgaatg cagtcaataa tatcctgcat
      601 gcccccttct gtggggtaca gcgacacata cagcggtagc ccgtctgcca gagcatcagt
      661 atcaagataa tgatccatca gcgctgtcag gggctcatct gacaacaacg gctgagccat
      721 cagattgtca aacccgttga ttgtcgatat atggcgaaga agcgtaat
//
LOCUS       DAZGTR010000441          767 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGTR010000441 DAZGTR010000000
VERSION     DAZGTR010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.445"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..275
                     /gene="yddL"
                     /locus_tag="ACHLCY_005472"
                     /pseudo
     CDS             1..275
                     /gene="yddL"
                     /locus_tag="ACHLCY_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            314..>767
                     /locus_tag="ACHLCY_005473"
     CDS             314..>767
                     /locus_tag="ACHLCY_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0372450.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRN"
BASE COUNT          270 a          156 c          134 g          207 t
ORIGIN      
        1 tgaaattaaa aatagttgcg gtggttgtaa ctggtttgtt agctgcgaac gtagcgcacg
       61 ctgccgaagt cgataacaag gatggtaata aactcgacct ttatggcaag gttaccgctc
      121 tacgttattt tactgatgat aagcgtgacg atggtgataa aacttatgcc cgtctcggct
      181 ttaaaggaga aacgcaaatc aatgatcaaa tgattggttt tggtcactgg gaatatgatt
      241 ttaaaggcta taacgatgaa gccaacggct cgcgcggcaa gaatctctga ttgagttaag
      301 tgcccatcac aatatgatca ctgaccttat attacacaat catcccagga tgaaaacaat
      361 cactttaaac gacaaccata ttgcacattt aaacgccaaa aacactacaa aactggaata
      421 tttaaactta agcaataaca atttactgcc aaccaatgac attgatcaac taatatcatc
      481 aaaacatctt tggcatgtat tagttaacgt catcaacaat gatccacttg cacaaatgca
      541 gtactggact gcagtaagaa atataattga tgacactaat gaagtgacca ttgatttatc
      601 aggacttaat ttaaccactc aaccaccagg gctgcaaaac ttcacctcta tcaatcttga
      661 taataaccaa ctcacacatt ttgatgcaac caactacgat agactcgtaa agctaagtct
      721 gaatagtaat gctcttgagt caataaattt tcctcaaggc agaaatg
//
LOCUS       DAZGTR010000442          763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGTR010000442 DAZGTR010000000
VERSION     DAZGTR010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.446"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(172..744)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLCY_005474"
     CDS             complement(172..744)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLCY_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP0372451.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          219 a          185 c          159 g          200 t
ORIGIN      
        1 caatgacaac gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg
       61 cggtaaataa cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg
      121 acataacgac tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt
      181 atattcgaca gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt
      241 tgccacatag cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata
      301 agcagagtcg ttataaagtt taacctgcga attatcggac tggttaaaaa tacgaatacc
      361 aacgccggtt gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt
      421 aagcggcgta cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg
      481 aacggctgct tgagtgtacg ttgccgggca attttcaagc ttaatactga acttctgtgg
      541 cgacgccaga ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat
      601 ttgatcggct gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg
      661 aacaacacca tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc
      721 accgcctata atattacgct tcataaaaac tccattgata taa
//
LOCUS       DAZGTR010000443          752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGTR010000443 DAZGTR010000000
VERSION     DAZGTR010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.447"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>752
                     /gene="espL2"
                     /locus_tag="ACHLCY_005475"
     CDS             <1..>752
                     /gene="espL2"
                     /locus_tag="ACHLCY_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFP0372452.1"
                     /translation="LKCYGLKEDTLSLFVDKTKSNDNNNVFIKKLPDNILQDVVINFA
                     MGAGLREIIKKVYNDTRFTDLTKSQMKILCESKNVNNVPGLLLALQNGHDNVIDEYGT
                     LIKKSNLNKEELIHILSARTLDGTIPGLYQALQNGHAQAIKSYGNLVLDTIDKNIDLE
                     YLLSAFKYEAHSSSKYTPGLFSAFKNGHADAIKAYCGVLGNSNLTRGEIIRMLEARNY
                     DGAPGLLLAYQNGDINTIQSFFDSLIMLDISK"
BASE COUNT          276 a           96 c          138 g          242 t
ORIGIN      
        1 ctgaaatgtt atgggctaaa agaagacaca ctgtcacttt ttgtggataa aacaaaatcg
       61 aatgacaaca ataatgtgtt tataaaaaaa ttacctgata atattctcca agatgttgtt
      121 attaattttg ctatgggagc aggactaaga gagataataa aaaaggtata taacgatact
      181 cgatttacag atcttacaaa atctcagatg aaaatcttgt gtgaatcaaa aaatgttaat
      241 aatgttcctg ggttgctttt agctctacaa aatggacatg ataatgtaat tgatgaatat
      301 ggtactttaa ttaaaaagtc aaatcttaat aaagaagaat taatacatat attatctgca
      361 agaactttgg atggaaccat acctggttta tatcaggccc ttcaaaatgg acatgctcaa
      421 gctattaaga gttatggtaa tttagttctg gatactattg ataaaaatat agatttagag
      481 tatttgcttt cagcatttaa atatgaggcg catagctcta gtaaatatac tcctggatta
      541 ttttctgcat ttaaaaatgg tcatgcagac gcaataaaag catattgtgg cgtacttggc
      601 aatagtaatt tgacaagagg tgagataatc aggatgcttg aggcgagaaa ttatgatggt
      661 gcaccaggat tacttcttgc gtatcaaaat ggagatatta atacgattca gtcttttttt
      721 gactcactaa taatgttaga tatatcaaag ga
//
LOCUS       DAZGTR010000444          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGTR010000444 DAZGTR010000000
VERSION     DAZGTR010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.448"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..649)
                     /locus_tag="ACHLCY_005476"
     CDS             complement(<1..649)
                     /locus_tag="ACHLCY_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0372453.1"
                     /translation="MIIDAHTHIGDFVKIRMPEDVFLASLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHSKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSDKVQEYIRLAQKYDLPVVSHTSNDYESSPQLVYEMALKY
                     PNVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQSIKICGSDKILFG"
BASE COUNT          243 a          161 c          121 g          221 t
ORIGIN      
        1 taccaaataa aatcttatca ctaccacaga tttttatcga ctggtaaacc atatccgccc
       61 taacccacgc agtatcgcca tagagattag gaagttttgc aatcaattcg atcgcttcct
      121 gattatctgt cgcgagcccc atatgacaca taacaaaatt cacatttgga tatttaagtg
      181 ccatctcata aaccaactgt gggctggatt cataatcatt agaagtatgg ctaactaccg
      241 ggagatcata tttttgagca aggcgtatat attcctgaac tttatctgaa ttgaaagcca
      301 tttttgaatg atatgggtga actttgatgc cataaatgat atctcgattg ctggcaatca
      361 tatcttcaaa atcttgatca caactttcca gacgaggctt aatccacata aacgcgccaa
      421 ttcgctttga atgttgacga acaagtctga gcatacgttc attattatca tgctgtgtaa
      481 cttgatcttc atccggaatc gggttctggt catggtcaac ttctactgca gatccacaag
      541 aacataatgc aaaatcaata ttatacttgt ccagagatgc cagaaaaaca tcttctggca
      601 tacggatttt cacaaagtca ccaatatgag tgtgtgcatc aattatcata acaaacatcc
      661 taattgaaat ttaaaaatca taaagatcag cagcaggtaa accttcaatt tcataactga
      721 aaccggagat ctccatactt cttccg
//
LOCUS       DAZGTR010000445          743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGTR010000445 DAZGTR010000000
VERSION     DAZGTR010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.449"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..245
                     /locus_tag="ACHLCY_005477"
     CDS             <1..245
                     /locus_tag="ACHLCY_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0372454.1"
                     /translation="VNTGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANT
                     QSGDKGAYTCEVTDSAEQPQSITSDACTVTVNGAGG"
     gene            251..625
                     /locus_tag="ACHLCY_005478"
     CDS             251..625
                     /locus_tag="ACHLCY_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP0372455.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREPVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            649..>743
                     /locus_tag="ACHLCY_005479"
     CDS             649..>743
                     /locus_tag="ACHLCY_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372456.1"
                     /translation="MRLALRLGRTLSELRHSLSASEAMMWMEFDRV"
BASE COUNT          167 a          175 c          248 g          153 t
ORIGIN      
        1 ccgtgaatac cggtgcgctg ctgacaatgt cagtcagtgt caacggggga acgccgcctt
       61 ataaacacgc ctggaagaag gatggtcagc cggtagaggg acagactact gacactttca
      121 gtaaagccaa tacgcagtca ggtgataagg gggcttatac ctgcgaggta acggattctg
      181 cagaacagcc gcagagcatt acctctgatg cgtgtacagt aacggttaat ggtgcgggcg
      241 gataaggctt atggcaaaag atctgaaaac actggcgctg gccagactgt cggggttccg
      301 tcataaaacg gtgaaggtgc cggaatgggg taatgtcagc gtggtgctgc gggagccttc
      361 ggcagaggcc tggtatctgt ggcaggaagt gctcaatggt gatggagagg atgacgatac
      421 cctgtcggtg gtggcgaaaa cccgccgtaa cctggaagcg gatgtgacgc tgttctgcga
      481 tgtcctgtgt gatactgacc tgcaacgggt gttcactccg gacgaccgtg agccggtgct
      541 ggccgtctat ggtccggtac atgcccggtt gctgcgtcag gcactggaac tgatcgctga
      601 tgcagagtcg gccagaaaaa agtagcccgc ccggaaattc gctttctgat gcgacttgcg
      661 ctccgtctgg ggcgcacctt atccgaactg cggcacagcc tgagtgcgag cgaggcgatg
      721 atgtggatgg agttcgacag ggt
//
LOCUS       DAZGTR010000446          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGTR010000446 DAZGTR010000000
VERSION     DAZGTR010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.450"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(398..529)
                     /locus_tag="ACHLCY_005480"
     CDS             complement(398..529)
                     /locus_tag="ACHLCY_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0372457.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLL"
     gene            complement(526..731)
                     /locus_tag="ACHLCY_005481"
                     /pseudo
     CDS             complement(526..731)
                     /locus_tag="ACHLCY_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299632.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="tellurite resistance protein"
BASE COUNT          203 a          214 c          150 g          164 t
ORIGIN      
        1 ctgtagtccg gaagcctgaa tgacactcgg ggactacagc ccaagaaatg aaaaaaggcc
       61 acgcagttgc gcagcctgat aaaccctggt taaaatccac acgataacaa cacaacaata
      121 tcagtatctc atgctattgc ccgaacccat tcgggcattt tttacccata aaaaaagccc
      181 ctccggagag gggcatgttt gcatgcacat tctttttctt gcatggtgcc gggtgcctcc
      241 cggtgaattc agtatcagca cctgaatccg cgattatcac atatacctgg ttgctgattg
      301 cccctccgca cagggggatt caccatgcag tagtattttt aataaacagc aaataaaaaa
      361 atcaagcatt atgcaggctg tttcttttta tcaccggcta cagcaatacc acaatgccgc
      421 agaccagcac cccatccgcc agcaccgaca tgattctgct ggtgaaatcc accatcacca
      481 ccagaaacag caggagtgca gccacagcca ggcgcagttt taccgtcact ggtgattctc
      541 cagacgaaga cccagaacac cggcaatctc ttccagcacc ttgcgctctt ccggctcaat
      601 tatttctgac gtaaacgcgg acagcgcagg ctgattacgc agtacctgtt cgatcttcga
      661 tttctaggaa gcctcacatt caccatctgc acaggccacc aggtaggcag cattaataac
      721 cgcctgtgcc a
//
LOCUS       DAZGTR010000447          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGTR010000447 DAZGTR010000000
VERSION     DAZGTR010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.451"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..251
                     /gene="rrrD"
                     /locus_tag="ACHLCY_005482"
     CDS             <1..251
                     /gene="rrrD"
                     /locus_tag="ACHLCY_005482"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0372458.1"
                     /translation="RQINPYINVDIPETTRGALYSFVYNVGAGNFRTSTLLRKINQGD
                     IKGACDQLRRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            468..653
                     /locus_tag="ACHLCY_005483"
     CDS             468..653
                     /locus_tag="ACHLCY_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0372459.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPCVKPPSPPAWVMQPV
                     PDWQKPLNGIISSSENG"
BASE COUNT          189 a          184 c          193 g          160 t
ORIGIN      
        1 ccagacaaat taacccgtac atcaacgtcg atataccgga aacaacgcgc ggcgctcttt
       61 actcgttcgt ttacaacgtg ggcgctggca atttcagaac atcgacgctt cttcgcaaaa
      121 taaaccaggg cgatatcaaa ggcgcatgtg atcagctacg gcgctggaca tacgctggcg
      181 gtaagcaatg gaaagggctg atgactcgcc gcgagattga gcgtgaagtc tgtttgtggg
      241 ggcaacaatg agcagggtaa ccgttattat ctccgctctg gttatctgca ttatcgcctg
      301 cctgtcatgg gctgttaatc attaccgcga taacgccatt acctacaaag cgcaacgcga
      361 taaaaaagcc agtgagctga agctggcgaa cgtgacaatt actgatatgc aggtacgcca
      421 gcgtgatgtc gctgcacttg atgccagata ctcgagggaa ttagccgatg cgagagctga
      481 aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag cgcctgcgca tcaacgccaa
      541 ctgtccaggc tccttgcgta aagcccccat cacctccggc gtgggtaatg caaccggtcc
      601 ccgactggca gaagccgctg aacgggatta tttcatcctc agagaacggc tgatggcaat
      661 gcagaagcaa ctggaaggag cacaggaata tatccgtacc cagtgtatac cgtgatgttt
      721 tgttat
//
LOCUS       DAZGTR010000448          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGTR010000448 DAZGTR010000000
VERSION     DAZGTR010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.452"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(212..682)
                     /gene="ygeG"
                     /locus_tag="ACHLCY_005484"
     CDS             complement(212..682)
                     /gene="ygeG"
                     /locus_tag="ACHLCY_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0372460.1"
                     /translation="MFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIYAYAFDYH
                     EKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYNYSPYDDY
                     SVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDNG"
BASE COUNT          223 a          114 c          114 g          271 t
ORIGIN      
        1 tcactaattt atcttaggaa ttcagtgatg taatctgaaa tagtcttatt caattttgat
       61 aaagtagatt gttctctaac gcattgaaaa ataatgattt atagtatgct cttaactgtt
      121 ttaagatgct gatttaagaa aacataagag gtgctggtta tagtggttaa ctgcactata
      181 accagcaaat ttaaccaaat gattgtaatg cttagccatt atcttctgaa ttatcgttta
      241 agagttcaat atatgcctgc gctttactct taacactatc atcctcacaa ttgttaataa
      301 tgtgatagaa acattgcatt gcgttatcga tatttttagc cccaatctga cattgaccca
      361 tacgataaat aactgaatag tcatcatacg gggagtaatt gtaacttagt ttgtaaaggt
      421 catatgcttg ttgatatttc tttttgggct gacatactga tgcaaaatct tttagatact
      481 catgattttc gaacgcataa atgcacaaaa atttataata aatttctgcg tccgttatat
      541 tacccttttc atggtagtcg aatgcatatg catatattct atcaaggata tcaggagtta
      601 aaccatgtag taatgccaga ttttctcctt tcgataatgc gtgttttagt tgatttgccc
      661 attcatcact attattgaat atttaaattg tttctgtgct cataatattc ctctgtgatg
      721 tg
//
LOCUS       DAZGTR010000449          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGTR010000449 DAZGTR010000000
VERSION     DAZGTR010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.453"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>709
                     /locus_tag="ACHLCY_005485"
     CDS             <1..>709
                     /locus_tag="ACHLCY_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372461.1"
                     /translation="LIQLQRNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRM
                     VRETADSTTDQLQNKTLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIR
                     GRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGTADVD
                     KWALYAIGQYCDQRVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQ
                     TLTFVQDRPSDVVWPYTN"
BASE COUNT          160 a          195 c          224 g          130 t
ORIGIN      
        1 gctgattcag ttacagcgta acggtaactg ggtgacagaa aaggatgtca ccattaacgg
       61 caagaccacc tcgcagttcc tggcgtcggt gattctggat aatctgccgc cccggccctt
      121 taacatccgg atggtcaggg agacggcgga cagcaccacg gaccagctgc agaataagac
      181 gctgtggtcg tcatacaccg aaatcatcga tgtgaaacag tgctacccga acacggccat
      241 tgtggggctg caggtggatg cggagcagtt cggcggccag cagatgacgg tgaactacca
      301 tatccgcggt cgcatcatcc aggtgccgtc aaactacgac ccggaaaaac gcacttacag
      361 cggtatctgg gacggcagtc tgaaaccggc atacagcaac aacccggcct ggtgtctgtg
      421 ggacatgctg acccacccgc gctacgggat ggggaaacgc ctggggaccg cggacgtgga
      481 caaatgggcg ctgtatgcca tcgggcagta ctgtgaccag cgtgtcccgg acggcttcgg
      541 agggacagag ccgcggatga cctttaatgc gtacctgtca cagcagcgta aggcgtggga
      601 tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg gccagacgct
      661 gacgttcgtt caggaccgcc cgtcggatgt ggtgtggccg tacaccaac
//
LOCUS       DAZGTR010000450          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGTR010000450 DAZGTR010000000
VERSION     DAZGTR010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.454"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>709)
                     /locus_tag="ACHLCY_005486"
     CDS             complement(<1..>709)
                     /locus_tag="ACHLCY_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0372462.1"
                     /translation="WDVSKETFPKDKFAGYVKSVFSKIDFYKQCGVINPQNANTAYFG
                     DTDGRVGAVLYALLVSGHIGIREKGWSLLCELLNHENMASSAYKHKNNKVLYELLNTR
                     DMILNELHKHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMESNKDSR
                     INKNVVVLAGNHEINFNGNYTARLANHKLSAGDTYNLIKTLDVCHYDSERKVLTSHHG
                     IIRDEENACYCLGALQVP"
BASE COUNT          222 a          147 c          109 g          231 t
ORIGIN      
        1 ggaacctgca aagcaccgag gcaataacat gcattttctt catctcttat tattccgtga
       61 tgactggtta aaactttcct ttctgagtca taatgacaaa catcgagcgt tttaattaag
      121 ttatacgtat caccggcaga gagtttatga tttgctagtc gtgcagtata gttaccatta
      181 aaattgattt catgatttcc agccagaacg acgacatttt tattaatacg gctatcttta
      241 ttactctcca tatttcgcat agaatttagc aaagtaagga tatatttatc accaaaaata
      301 gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat cgcatcgtcc
      361 tttaaaaaaa catgtttgtg caactcattc aatatcatgt ctctcgtatt taataactcg
      421 tagagcactt tattattttt atgcttgtat gcagatgaag ccatgttctc gtgatttaat
      481 aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga aacgagcaat
      541 gcataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt gttggcattt
      601 tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac agatttaaca
      661 tagccagcaa acttatcctt aggaaacgtc tctttagaga catcccaac
//
LOCUS       DAZGTR010000451          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGTR010000451 DAZGTR010000000
VERSION     DAZGTR010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.455"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..620)
                     /locus_tag="ACHLCY_005487"
     CDS             complement(<1..620)
                     /locus_tag="ACHLCY_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0372463.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          172 a          172 c          168 g          190 t
ORIGIN      
        1 ggcaccacgc cgatggtgtt ttcgtcacag gcttcaatca tgcgtttcgg gtccataaac
       61 aactgaccgg ggcgcatagg gatctcacgc agctccacat cccagtagcg ggcgaattta
      121 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgttgg tttgcctgca
      181 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      241 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggcgcagg cgcatgccac
      301 agatcggcaa ccatatttac gcaacgcagg tcgatggctg cggattgcgg atattcttct
      361 ttgtcgatcc agtttttgtt aatggataaa tccatcaatt tgtggacatt ttcgtcgtcc
      421 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcgtca
      481 ttgataatct ggaatgcgac atcgtcgcgc atttcgtgca gcggaaaacg ttttgattct
      541 gcgatagtgg aaatagactt cgcaccaaaa cgtgaatcga gtagttccga ccttaaatcc
      601 gttacttgct tcttatccat tttaaactcc ttaaaatgat tggatcgcat taaaaaagta
      661 ggatttatcg ataaagtaag caagtgcaaa ggactcgtgt tt
//
LOCUS       DAZGTR010000452          693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGTR010000452 DAZGTR010000000
VERSION     DAZGTR010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.456"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>693
                     /locus_tag="ACHLCY_005488"
     CDS             <1..>693
                     /locus_tag="ACHLCY_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0372464.1"
                     /translation="AAQNALNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQN
                     QEQARESARQYALTIDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSL
                     QEKIAGYQYVLANPGWTTGDGFMINHLTSVKTVTEGLSLATEQLAVEQSRLAQMQEKV
                     QSIQDVLAGLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQG
                     LVNVPLRLPQATL"
BASE COUNT          168 a          167 c          223 g          135 t
ORIGIN      
        1 cgcagcccag aatgcgctga acagtacaac ggcggtgggc tcacgtctga tgactggtgc
       61 gttgggactg gttggtggcg tacccggact ggtgatgctg ggggcagcag catggtatac
      121 gctgtaccag aatcaggagc aggccaggga gtcagcgcgc cagtatgcac tgacgataga
      181 tgaaatcgcg cataaaacgc cgtcaatgtc tttgcctgaa gcctcagata atgaaggacg
      241 aacacgggcg gcgctggcag agcagaaccg gctgattgat gaacaggcca gccgggtgaa
      301 atccctgcag gaaaaaatcg ctggatatca gtatgttctg gctaaccctg gctggacaac
      361 cggtgacgga ttcatgataa accatctgac atcggtgaag accgtgacgg aagggctttc
      421 tctggcaaca gagcagcttg ccgttgagca gtcccgtctg gcacagatgc aggaaaaagt
      481 gcagtccatt caggatgtgc ttgccgggct ggaagaccgt cgtgtggcgt taattcgtca
      541 gcaggcggca gagcagaata aggtgtacca gtccatgctg gttatgaacg gtcagcatac
      601 ggaattcaac cgtctgctgg ggctggggaa tgaactgctt cagcagcgtc agggactggt
      661 gaatgtaccg ttacggctgc cgcaggccac cct
//
LOCUS       DAZGTR010000453          680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGTR010000453 DAZGTR010000000
VERSION     DAZGTR010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.457"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>680
                     /locus_tag="ACHLCY_005489"
     CDS             <1..>680
                     /locus_tag="ACHLCY_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP0372465.1"
                     /translation="LTEISKMPEDSIFTKINTRIPRDTMPILRRYHLWPDGWNIRGLN
                     GFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSLPDTDKIGGIL
                     VKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVLGEEFINERKL
                     SDKAYIGVYKTTGTGKYDWLTPESIGVNDVTPADESTWCIGKGRCVDDFLFKDVSTLK
                     TENLPELF"
BASE COUNT          233 a          118 c          144 g          185 t
ORIGIN      
        1 attaacagaa atatctaaaa tgccagaaga tagtattttt actaagatca atacaaggat
       61 tcctcgagac acaatgccca tccttcgtcg ttatcaccta tggcctgatg gatggaatat
      121 tcgtgggctc aatggattca tgctatcaca taaaggtagt gaagtgattg atgctgttat
      181 cgcaggccag aatcaggctt acagggaact aagaagaata agagataata ttcatagtga
      241 aatatacttc aaacaaactg atgaattgtc ctcacttcca gatacagaca aaattggagg
      301 gattctggta aaaaaatacc tttcaggaag tcttttttca aaattcagac aagatactat
      361 tatcccggag gcattgagca ccctccagat atcaggtcct gacttgattc aaagaaagat
      421 gttgcaattt ttcaggagta gaggggtgtt aggtgaagag ttcattaatg aaagaaaact
      481 gagtgataaa gcttatattg gtgtctacaa aacaactggc acagggaaat atgactggtt
      541 aacccctgaa tcgatcggcg ttaatgatgt cacgcccgca gatgaaagta cctggtgtat
      601 aggaaaaggc cggtgtgttg atgacttcct gttcaaagat gtttcaacac taaaaacaga
      661 aaatcttcca gaattattct
//
LOCUS       DAZGTR010000454          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGTR010000454 DAZGTR010000000
VERSION     DAZGTR010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.458"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>675
                     /locus_tag="ACHLCY_005490"
     CDS             <1..>675
                     /locus_tag="ACHLCY_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0372466.1"
                     /translation="DKLLAASRGEFVAGISDPNDPKWVKGIQTRDSVNQNQHESERNY
                     QKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVMGDATYQET
                     FDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHDSDNETGETADLSIKVNGHQEITS
                     TSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWL"
BASE COUNT          213 a          154 c          184 g          124 t
ORIGIN      
        1 ccgataagtt gcttgctgcc tctcgcggag aatttgttgc agggattagc gacccgaatg
       61 atccgaaatg ggttaagggg atccagaccc gcgattctgt aaaccagaac cagcatgaat
      121 cggaacggaa ctaccaaaaa gcggaacaaa acagcccaaa tgcgttacaa aacgagccag
      181 aaacgaaaca gcctgaacca gtggcgcaac aggaagtgga aaaagtctgc accgcctgcg
      241 gtcagaccgg cggcggcaac tgccctgatt gtggcgcggt gatgggcgac gcaacatacc
      301 aggaaacatt cgatgaagag tatcaggttg aagttcagga agatgatccg gaggaaatgg
      361 aaggcgctga acatccacac aaggagaaca ctggcggcaa tcagcatcac gatagcgata
      421 atgaaactgg cgagacggca gatctctcaa ttaaggtgaa cggtcatcaa gaaatcacat
      481 ccaccagcag agcggggatt catctgatga tcgaccttga aaccatggga aaaaatcctg
      541 atgccccgat catctcgata ggtgcaatat ttttcgatcc gcaaaccgga gatatgggac
      601 cggaatttag taagactatt gatctggaaa ctgctggcgg agtcattgat cgggacacca
      661 ttaaatggtg gctta
//
LOCUS       DAZGTR010000455          675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGTR010000455 DAZGTR010000000
VERSION     DAZGTR010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.459"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..364)
                     /locus_tag="ACHLCY_005491"
     CDS             complement(<1..364)
                     /locus_tag="ACHLCY_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0372467.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQR"
     gene            complement(416..625)
                     /locus_tag="ACHLCY_005492"
     CDS             complement(416..625)
                     /locus_tag="ACHLCY_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP0372468.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
BASE COUNT          146 a          221 c          155 g          153 t
ORIGIN      
        1 tccgctgact gatatccatc agcactttct gtgatgacga aaattcatcg gatgactgtg
       61 atgcctgttt cagacgggca tttacggaac tccactcatc cgccagtgaa atcaggtgtc
      121 cggtggcaaa ggcaccggca aacgcaccgg tcattccgac agcagaaccg cgaatttccg
      181 tcaactggct gttcagttct gccagggcac gtcgctgctc ccgggctgcc gcagcggcct
      241 gacgcccgcc attctgcagg gtccggtaat attcactgcc catacgggac gcccgctgga
      301 tctccgactg gaatgactgc gaatttgccg aaattttgat aatcagttca cgtaacgtcg
      361 ccattcacct ttctccgggc aaaaaaaacc tgccacagca ggttttcatc attatttatg
      421 acattgctgc aaggctcagc gcgtcttcca gcgccgcaaa cggatccacc tccggcttat
      481 cctcatcctc gccccagcag agcatggcgt ccttcagtgc aacattcatc ccctgtgccc
      541 caaaaaccgc tttcacgatc tgtgcattac ggatatcccc gcgctcatca cccagcgggg
      601 ataccctgtc gaactccatc cacatcatcg cctcgctcac actcaggctg tgccgcagtt
      661 cggataaggt gcgcc
//
LOCUS       DAZGTR010000456          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGTR010000456 DAZGTR010000000
VERSION     DAZGTR010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.460"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..439
                     /locus_tag="ACHLCY_005493"
     CDS             <1..439
                     /locus_tag="ACHLCY_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion protein EspJ"
                     /protein_id="HFP0372469.1"
                     /translation="RDFVAVRIQSNQFTDLKNKTIQGHKDTVAKVIDWYNPQKNAFGI
                     MMGTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNANLQKKYGTTENSSWVSASV
                     GSLLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVNIHPLKK"
     gene            complement(452..>667)
                     /locus_tag="ACHLCY_005494"
     CDS             complement(452..>667)
                     /locus_tag="ACHLCY_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372470.1"
                     /translation="IQCTLANTFPNEHPEAFILVTSAEKFIVDGNCIQLIGIGNEQGE
                     FDFYRQIYPVTGGEHKVEALMWTHLPA"
BASE COUNT          236 a          137 c          121 g          173 t
ORIGIN      
        1 acgggatttc gttgctgtaa gaatccaaag taatcagttt actgatttaa aaaacaagac
       61 aattcagggt cataaagaca ctgtcgcaaa agtcatagac tggtataacc cacaaaaaaa
      121 cgcatttggt atcatgatgg gcacaccaag acgttctgct gatatagcaa aggaagaatc
      181 cagaaacgct ctaaatttca tgataatgga aaaggataca tttaatgaaa agatacttaa
      241 cagcaatgct aatttacaaa aaaaatacgg cacaacagaa aatagtagtt gggtatcggc
      301 ttctgttggt tctcttctgg acaaaggagc caaagtatat ccagatatat catgctctct
      361 gagactggga gaaccattca ttattacact tccagaaaca gtcaggctgg atgttaatat
      421 ccacccactc aaaaaatgat cctgaacgtt tttatgcagg caaatgcgtc cacatcaagg
      481 cttcaacctt gtgctctccc cctgtcacag gataaatttg cctgtaaaaa tcaaactctc
      541 cctgctcatt accaatacca atcagttgaa tgcagttgcc atctacaata aatttttccg
      601 ctgacgtaac caaaataaag gcttccggat gttcattagg aaaggtgtta gcaagagtac
      661 actgaat
//
LOCUS       DAZGTR010000457          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGTR010000457 DAZGTR010000000
VERSION     DAZGTR010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.461"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(25..>667)
                     /locus_tag="ACHLCY_005495"
     CDS             complement(25..>667)
                     /locus_tag="ACHLCY_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0372471.1"
                     /translation="LKPGSVVLATKCIRALFLMHYGKVKIFDINHSIVSQYLEIQHKL
                     TRTHLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFL
                     SGCLSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEER
                     MRVAVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          208 a          152 c          129 g          178 t
ORIGIN      
        1 caaaaaataa taatcagatg aaaatcaata atcaagccct tgcctggata catatctgtc
       61 tggcgggaag ccataatagc ggtgaaatac agaaataaag taggaccagc ttgaataacc
      121 gcatttttct gctacagcct gtccatatcc atgccgggaa cataacatat tcacagcaac
      181 acgcatccgc tcttccagca acaagcgact gaacatgtgc ccttcatttt tcagttttat
      241 ctttaacaaa ctctcactca tatgcaggcg taacgcaatc gcaccaagcg tccagcttgc
      301 tgatatatct gtctgaatta tcgccctgac tttggcactt atactggata aacatccact
      361 taaaaataat aatatccgtt catctgattc aaacagcgac aggcatgcca tcataagaaa
      421 catatccgtg gtatctccgg aaaatctctg gctggtaatt aaagcctcag ccaacgcagg
      481 gttgttgggt tccagtgaca gataaagcgg aacgtcagtc agatgagttc ttgtcagctt
      541 atgctgaatt tccagatatt gacttactat ggaatggttt atatcgaaaa ttttaacttt
      601 gccataatgc ataaggaaaa gcgccctgat gcatttggtg gccagaacga ctgagccggg
      661 cttaagt
//
LOCUS       DAZGTR010000458          665 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGTR010000458 DAZGTR010000000
VERSION     DAZGTR010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.462"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(89..631)
                     /gene="fimA"
                     /locus_tag="ACHLCY_005496"
     CDS             complement(89..631)
                     /gene="fimA"
                     /locus_tag="ACHLCY_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0372472.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          196 a          145 c          140 g          184 t
ORIGIN      
        1 atttacattg ccaccatacc gcttcagaca accggtacat tactttgtta taagacgtga
       61 tatataattc aaaacaacgt ggttttgatt attcgtatct cataataaaa gtcgcgtcag
      121 catttgcttg ccctgctgat gcacttgtac cggtgccttt ataacgtgca gaaaaatgaa
      181 gaacgttggt cccggggatc aagttctggt tggttgagaa gctattccca tcaggtttga
      241 gaattgcgga tgtattatca agtatttcaa taccgacacc cgttgctgtt gttgtattgg
      301 tactggatgc aatggccaat aaattatcat tgattgcatc agcctgacca gaaaatgcaa
      361 cggcagcatt agcggcaaca acaggatcgc agtcgttcaa ctgaatggtg aatggaatta
      421 atgcagaggt attaccaaca gcattgaaaa tatcggtacg atattgaccg agtgtgacaa
      481 cctggtctgc cgagtcagta ttcactgaac aggcagcatt caccagttcg ccttcaaagt
      541 gaattgtacc gccatctaca acggcagcat aactacccgt agcggcgaat agtgcggcag
      601 ccagcgcaga cgaaataaat cttaatttca tatatattcc ttcaatctca tttatcgact
      661 ccaca
//
LOCUS       DAZGTR010000459          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGTR010000459 DAZGTR010000000
VERSION     DAZGTR010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.463"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..584)
                     /locus_tag="ACHLCY_005497"
     CDS             complement(<1..584)
                     /locus_tag="ACHLCY_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0372473.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEAVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFD"
     gene            complement(584..>658)
                     /locus_tag="ACHLCY_005498"
     CDS             complement(584..>658)
                     /locus_tag="ACHLCY_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372474.1"
                     /translation="CREWSVTDNARYSDFSCTIEQVVN"
BASE COUNT          140 a          240 c          148 g          130 t
ORIGIN      
        1 ttgtcgaact catccgccac agccccgccc gtgtaaccac actcatcaga gcggtaggtc
       61 cacatacagg tattcgccag catgatgcga ccgggaaaca gcgccccatc cgtctcggtc
      121 ggtgtagcca gcacaaacga ggccgtcatg gctgtcagct gcgacatctg ctccaccacc
      181 cagcggtcac tcagctcctg ctccgggtcc gcctccggat tgcccgcaac gaaattcacc
      241 gcatccagaa aacgggcata cacccggcgg cggaccaccg tggcccccac cagactctgc
      301 aggtcctccg ccatcccggt gacaaggcca aacagattgg acaccgtcag tgacgggcgg
      361 gcactgctgc ccttcccgtt catctcaaaa ccgctgccgt caatcgggta tgcctgatat
      421 tgccgcccct gccaggtgac cgcctcccct ttttcattca gctcattaca gaaaaaatac
      481 cgctcaccac cctgcaccgt caggtcgatt tcccagagta ccacccgcgg tgactgctct
      541 gatttaaccg actcgttcag actttcttca tgaatgtcct gcatcagttc accacctgct
      601 caatcgtaca actgaaatca ctgtaccggg cgttatccgt gacgctccac tcccggca
//
LOCUS       DAZGTR010000460          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGTR010000460 DAZGTR010000000
VERSION     DAZGTR010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.464"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>657
                     /locus_tag="ACHLCY_005499"
     CDS             <1..>657
                     /locus_tag="ACHLCY_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0372475.1"
                     /translation="VYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSATGPDGLE
                     LRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTF
                     T"
BASE COUNT          169 a          186 c          197 g          105 t
ORIGIN      
        1 agtgtatgac cgcagcaata cacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagc tacacgtatc agtatgagaa
      181 ggaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggtga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgatgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggcagga caacagagta
      361 cagcccggat gtggtgacgg gactcatcac gcgcatcacc acgccggatg gcagggcatc
      421 ggcgttttac tataaccacc acaaccagtt aacgtcagcc accgggcctg acgggctgga
      481 attgcgccgg gaatatgatg aatccggccg tctgattcag gaaactgccc ctgacggcga
      541 tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa cggaagatgc
      601 caccggcagc cggaaaacca tgacgtggag ccgttacggc cagttgctga ccttcac
//
LOCUS       DAZGTR010000461          652 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGTR010000461 DAZGTR010000000
VERSION     DAZGTR010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.465"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..376)
                     /locus_tag="ACHLCY_005500"
     CDS             complement(<1..376)
                     /locus_tag="ACHLCY_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0372476.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAP"
     gene            complement(373..>652)
                     /locus_tag="ACHLCY_005501"
     CDS             complement(373..>652)
                     /locus_tag="ACHLCY_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0372477.1"
                     /translation="NMEATGFYRISLPSAQPGDILLCCFGASVPNHAAIYCGNGELLH
                     HLPEQLSKRERYSEKWQRRTHSAWRHRHWHASAFTGIYNDLAAASACM"
BASE COUNT          140 a          207 c          182 g          123 t
ORIGIN      
        1 tcggggccag catctgtgcc acaccaccca gtatcatgct ggcacccagt gaaaacagca
       61 tcgtggtggc agaaaaaccg ccggcactca gcgctgcacc ccaggctgcc atcgatgctc
      121 cggccgtgaa gaaagagccc acgatggctg ccgcccccag cacaatctgc agtccaccct
      181 ttccggcccc ggccagtcgc ggcacaatgt ggatgaccgt tccctcaccc agctgttcgt
      241 gaagacgggc gtacaccgcc tccggtgccg tgtcatcacc ggcaatacgt atctggtacc
      301 agccttcgtt catctgacgg cggaatccgg gcacctgtaa cgacagcgcc cggatggctt
      361 ccgctgccgt gttcacatac aggctgaggc ggcggccaaa tcgttgtaaa tccccgtgaa
      421 ggcagatgcg tgccagtggc ggtgacgcca ggctgaatgc gttcgtcgtt gccatttttc
      481 ggaatacctc tcccgtttac tcagttgttc aggcagatgg tgaagcagct caccgttgcc
      541 gcagtaaatg gcggcatggt tcggtaccga agcaccaaag cagcacagca gaatatcgcc
      601 aggctgtgcg gaaggcaggg aaatcctgta aaaaccagtc gcctccatat tg
//
LOCUS       DAZGTR010000462          650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGTR010000462 DAZGTR010000000
VERSION     DAZGTR010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.466"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(43..>650)
                     /locus_tag="ACHLCY_005502"
     CDS             complement(43..>650)
                     /locus_tag="ACHLCY_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFP0372478.1"
                     /translation="SPTAMVGRALAQAVTQTLRPAVTKAATQAGMGANGMRFAAMQSN
                     LMINHGKLTNQLLQAVAKQTGSSDTQQWFKQEQITFLSRTVNKTVDDYCMSNNSVINK
                     ETKFRIFKAVESAIQQPLDMNCAQSSIGHFLQSNKYFNQKVDEQCGKGVDPITRFNTQ
                     TKMIELVSREIFEQNFNTAKVSDIKALTQRAIAENVQDTRL"
BASE COUNT          166 a          157 c          127 g          200 t
ORIGIN      
        1 tgcatcaaga acaaacaaat aacagagcag taagtccctc ccctacagcc gagtatcctg
       61 cacattttct gcaatcgccc tctgagttag agccttgata tcactgactt tcgcggtgtt
      121 aaaattctgt tcaaagattt cgcgtgagac cagttctatc atcttagtct gagtgttaaa
      181 acgagtaatg ggatccacgc ctttaccaca ctgctcatca actttctgat taaaatattt
      241 attggattgc aaaaagtgac caattgatga ttgtgcacag ttcatatcga gaggctgctg
      301 tatcgcgctt tcaaccgctt taaaaatgcg aaatttagtc tccttattaa ttaccgaatt
      361 attactcatg caatagtcat ccacagtttt gtttactgtt ctggataaaa aagtaatctg
      421 ctcctgcttg aaccattgct gggtatcgct actacccgtt tgtttggcta cggcctgtag
      481 tagctggtta gtcagtttgc catgattaat cataaggttc gactgcatag ctgcgaacct
      541 catcccattg gcccccatgc ccgcttgcgt cgcagccttg gttactgctg gtctaagagt
      601 ttgtgtaacc gcctgagcta acgccctacc taccattgcc gttggactaa
//
LOCUS       DAZGTR010000463          645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGTR010000463 DAZGTR010000000
VERSION     DAZGTR010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.467"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>645
                     /locus_tag="ACHLCY_005503"
     CDS             <1..>645
                     /locus_tag="ACHLCY_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0372479.1"
                     /translation="VYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEICYRYDSDGRVT
                     EQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFD
                     AVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGPDGLELRRE
                     YDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFT"
BASE COUNT          165 a          180 c          196 g          104 t
ORIGIN      
        1 ggtgtatgac cgtagtggca aacaggtgcg cagctttact tacgatgata aataccgggg
       61 ccggatggtg gcgcaccgtc acacgggccg accggaaatc tgttaccgtt acgacagcga
      121 cgggcgggtg acagaacagc taaacccggc aggcttaagt tacacgtatc agtatgaaaa
      181 agaccgcatc accatcaccg acagcctgaa ccgccgtgaa gtgctgcaca cgcagggcga
      241 agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca cgcagagtca
      301 gtttgacgcg gtgggcaggc ttaaggcaca gacggatgcc gcaggccgga caacagaata
      361 cagcccggat gtggtgacgg gcctcaccac gccggatggc agggcatcgg cgttttacta
      421 taaccaccac aaccagttaa cgtcagccac cgggcctgac gggctggaat tgcgccggga
      481 atatgatgaa tccggccgtc tgattcagga aactgcccct gacggcgata tcacccgcta
      541 ccgttatgat aatccacaca gtgacttacc ctgcgcaacg gaagatgcca ccggcagccg
      601 gaaaaccatg acgtggagcc gttacggtca gttgctgagc ttcac
//
LOCUS       DAZGTR010000464          638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGTR010000464 DAZGTR010000000
VERSION     DAZGTR010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.468"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            72..638
                     /locus_tag="ACHLCY_005504"
                     /pseudo
     CDS             72..638
                     /locus_tag="ACHLCY_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
BASE COUNT          208 a           93 c          119 g          218 t
ORIGIN      
        1 atttggtcat gaaatttccg tcttgttaca ctactctaaa ctagagctta tggaattagc
       61 gggtggtatt catggaaaat aatgataaat tcttatcaca agacttattg gaatcttatg
      121 ccattcgttt gttgagcgga ccattgaatg gatgcgagta tgaaatactt aatgggcgtc
      181 tacttgttat cattggtaat gatgtttcgt taggaagaag tgatgctttt tctgagttac
      241 cagaaaacac aatagtcgtt ccttatggcg agcttacagg tagttttgag ataataatta
      301 ctaccgaccc tgatctggta gtaacattca gagaattaac agcacaagaa cctgaagata
      361 gaacgttaac attgaatcag caaatagaag ttttaggtct caaatttgct gtaaaagaaa
      421 aaaatgaagt ttggcagtat tcattgccag gcattattga aaataacatt atttctacaa
      481 aacaacattt ctttagcagt aagctgttta agtatgtaat gctttttttc tttttgctat
      541 cattttcatt gctttttata ttgttaatgc cagtaatgat ccgcagctga gacatatcga
      601 taaaattctt gtaaacaaaa acaggaatta tgaagttt
//
LOCUS       DAZGTR010000465          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGTR010000465 DAZGTR010000000
VERSION     DAZGTR010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.469"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..341
                     /locus_tag="ACHLCY_005505"
     CDS             <1..341
                     /locus_tag="ACHLCY_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372480.1"
                     /translation="CNRPSFVVSGDAGKITISENGKVTPPSHQHSEVLIEFAIDYLKN
                     NKKQGLMKCIGRCMGYLQIAAEIEALASGADKDAVVREALLREFDNPPFKKVPAYWFH
                     PGLTYLKGRI"
     gene            384..>633
                     /locus_tag="ACHLCY_005506"
     CDS             384..>633
                     /locus_tag="ACHLCY_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0372481.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDQVNAIERD"
BASE COUNT          165 a          141 c          171 g          156 t
ORIGIN      
        1 agtgtaaccg gccatccttt gttgtatccg gtgatgctgg gaaaataacc atctcagaaa
       61 atggaaaagt aacaccgcca tcgcaccagc atagtgaggt gctcattgaa tttgccattg
      121 attacctgaa gaacaataaa aagcaggggc tgatgaagtg cattggtcgt tgcatgggat
      181 atctgcagat agctgctgag attgaagcgc tggccagtgg tgcggacaag gatgcagttg
      241 tgcgggaggc tcttcttcgt gagtttgaca acccgccctt taaaaaagtg ccggcttact
      301 ggtttcatcc aggactgact tatcttaaag gacgtatata agctggctcg ttatctgttg
      361 ccgataaatc ctgataaata tccatgaaca ccaaaatcaa atacggcctg tcggctgccg
      421 ttctggcgct gattgccgct ggtgcgcctg cgcctgacat tctcgaccag tttctggatg
      481 aaaaggaagg taaccacacc acggcatacc gtgatggtgc gggtatctgg accatctgcc
      541 gaggtgccat catggtggat ggtaagcctg tgattcctgg catgaagctg tcgaaggaaa
      601 aatgcgacca ggttaacgcc attgaacgtg ata
//
LOCUS       DAZGTR010000466          633 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGTR010000466 DAZGTR010000000
VERSION     DAZGTR010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.470"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>633)
                     /locus_tag="ACHLCY_005507"
     CDS             complement(<1..>633)
                     /locus_tag="ACHLCY_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0372482.1"
                     /translation="ILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRVT
                     DTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVYT
                     TTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGEHLLMENLAMV
                     NPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAG"
BASE COUNT          135 a          196 c          164 g          138 t
ORIGIN      
        1 cctgcccagc ggtcagaacg cagggcaaga ccgatttcca cattggcgtg ttttgacatg
       61 aaaccgcgga aagtctcttc cccggcttcc cgggctttac gatactcccg gtacagaaag
      121 gcctcatcca ccgaataacc gagattcggg ttaaccatgg cgaggttttc catcagcagg
      181 tgctcaccgc tctccaccat ttcaggagga tgctcaaaaa tcaccggcag aaagtgcgga
      241 tcatgaattt tgccgtcacg gacatcccgg gcgtactgca gtttctgcct gaacaccccg
      301 gcaggcggtt cattcgactg ggtggttgtg tacaccacaa acccttccgg acgtgaagcc
      361 agcccgccga tggcttcacg taacatgtcc tccgctttgt actgcttgcc aaacagccac
      421 agttcatcaa tcagtgtccc cacggacttg ataccggaca ccgtattcgg atcggctgcc
      481 accaccttca gggtggtgtc cgtcacccga tgggtgatgg tccggatatg tgtctgcacc
      541 tgacagaggt catccagatc atcgtcccgt cgtaccatat ccctggcagg gttgaaggcg
      601 ttagccgcca cctccacggt cggggccaga atg
//
LOCUS       DAZGTR010000467          626 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGTR010000467 DAZGTR010000000
VERSION     DAZGTR010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.472"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>626
                     /locus_tag="ACHLCY_005508"
     CDS             <1..>626
                     /locus_tag="ACHLCY_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase family
                     protein"
                     /protein_id="HFP0372483.1"
                     /translation="KIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIK
                     DLAKNITQQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVDLTMKCYSPLCKNAS
                     RMDQNLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLE
                     GSSQERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYN"
BASE COUNT          219 a          106 c          136 g          165 t
ORIGIN      
        1 aaaattaagc atcttttaat tgttacgtcg acaattaatt tagaagtgtt taatgcactt
       61 aaacttgaaa agactaaatt aacattagtc acaatgacta tgcaggatca cgcaacatta
      121 aaagaaataa aagatttagc aaagaacata acacagcaat acggtacgcc ggatctttgt
      181 atagaggcga tgcgtaaaaa gaacctgaaa acgatgatat ttatcagtca gttgaaagca
      241 aaaacgaatt ttcaggttgt tgatttaacc atgaagtgct actccccttt gtgcaagaac
      301 gcgtcccgga tggatcagaa tctccgggct cccgtaccca tgacatgggc atttatgatg
      361 cgtgaggcgg gttttccagc agtcaggcca atatatgaat tgccactaag tgaggatgta
      421 ctcgatgagg tgcgcgagga aatgcgatcg ttaggatctt acattgcgct taatttagaa
      481 ggtagctcgc aggaacgtac attttcatta tcgattgcag aaaatctaat agcaaaaatt
      541 caaagtgaaa cagatatacc aatagtgatc gttcatggac caaaagggga agataaagcc
      601 agggtattag tggattgtta taataa
//
LOCUS       DAZGTR010000468          614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGTR010000468 DAZGTR010000000
VERSION     DAZGTR010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.473"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>614
                     /locus_tag="ACHLCY_005509"
     CDS             <1..>614
                     /locus_tag="ACHLCY_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFP0372484.1"
                     /translation="KLDISDEVFMPKMIDPVTVKDALYGLPDIIDANWQSDSESWRTI
                     KKDRKGGFYEKLWGQIPRNVGDTESIAKLKNNIISGCTGTLHSKIVQERYASLSFGET
                     DKISRSTRLDPNGFCPTLRAGTARDKGSFQAVRPIHPYHPRVITPREAARLQGFPDWF
                     RFHVTKWHSFRQIGNSVSPIVAEYILKGLYNLLNKRVQPEYLNH"
BASE COUNT          209 a          103 c          129 g          173 t
ORIGIN      
        1 tgaagcttga tatttcagat gaagtattta tgcctaaaat gattgatccg gttactgtaa
       61 aagatgcttt gtatggatta ccagatatta tcgatgccaa ttggcaatca gactctgaga
      121 gttggcgaac aattaaaaaa gatcgaaaag ggggatttta tgaaaaatta tgggggcaga
      181 tccctcggaa tgttggtgat acagaatcga tcgctaagtt aaaaaataat atcatatctg
      241 gatgtaccgg aacattacat agcaaaatcg tccaagagcg ttatgcttcc ttatcttttg
      301 gggaaactga taaaatttca agatctacaa gattagatcc aaatggtttt tgcccgactt
      361 taagggcggg aaccgctaga gataaaggaa gctttcaagc cgtcaggccg atccaccctt
      421 atcatccaag agtgattaca ccaagagaag ctgctagatt acaaggcttc cctgattggt
      481 ttcgctttca tgtaactaaa tggcatagtt tcagacaaat aggaaatagc gtgtcaccaa
      541 tagttgctga atatatatta aaggggctgt acaatttatt aaataaaaga gtacagcccg
      601 aatatttaaa ccat
//
LOCUS       DAZGTR010000469          609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGTR010000469 DAZGTR010000000
VERSION     DAZGTR010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.474"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>609
                     /locus_tag="ACHLCY_005510"
     CDS             <1..>609
                     /locus_tag="ACHLCY_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0372485.1"
                     /translation="GENESTEFDVTTLLRMDSERRMKTLGDAVKNTLLTPNEARKREN
                     LPPLAGGDALYLQQQNYSLEALSRRDAREDPFASSGKTVSAQLPDGASDGNKAISETE
                     HDAVKAMFRGDTEKMTERELSIIRALGEEFSTVLADLQRTFEGKMASQAQAFEEKLTS
                     LSAVLQKHVTVDEVRPVLQAMVDDAVGAIPVPRDGRDYDPDVL"
BASE COUNT          149 a          137 c          199 g          124 t
ORIGIN      
        1 ggggaaaacg agagtacaga atttgatgtc accacgctgc tgagaatgga cagtgagcgg
       61 cgcatgaaaa cgctggggga tgcggtgaaa aatacgcttc tcacgcccaa tgaggcccgt
      121 aaacgggaga acctgccgcc cctggccggc ggtgatgcac tgtatcttca gcagcagaac
      181 tacagtctgg aggcgctgtc ccgtcgtgat gcccgtgagg atccgttcgc gtcttccggg
      241 aaaacagttt cagcacaact gcctgacggc gcatctgacg gtaataaggc aatcagtgaa
      301 acagagcatg atgcggtgaa agcgatgttc aggggggata ctgagaaaat gacggaacgg
      361 gaactgtcca ttattcgtgc actgggagaa gaattctcca cagtgctggc ggatttacag
      421 cgcacatttg aggggaagat ggcctcgcag gcacaagcgt ttgaagagaa actgacttcc
      481 ctgtcggcgg tattacagaa gcatgtgacg gtggatgagg tgcgtccggt tctgcaggcg
      541 atggtggatg acgctgtggg ggccattccg gtaccgcgtg atggtcgtga ttatgatccg
      601 gatgtactg
//
LOCUS       DAZGTR010000470          601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGTR010000470 DAZGTR010000000
VERSION     DAZGTR010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.475"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..315
                     /locus_tag="ACHLCY_005511"
     CDS             37..315
                     /locus_tag="ACHLCY_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001177654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFP0372486.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMGMLLAVLEWGVEDEELAELAKKVARMLTKEKAPKNGEFFEA"
     gene            350..496
                     /locus_tag="ACHLCY_005512"
     CDS             350..496
                     /locus_tag="ACHLCY_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 family protein"
                     /protein_id="HFP0372487.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            489..>601
                     /locus_tag="ACHLCY_005513"
     CDS             489..>601
                     /locus_tag="ACHLCY_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HFP0372488.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVP"
BASE COUNT          202 a          125 c          147 g          127 t
ORIGIN      
        1 cattcaatca attgttatct aaggaaatac ttacatatgc aacttacaag tactcgcaag
       61 aaagcgaatg caattacaag caacatcctg aaccgaattg ctgtacgtgg ccagcgaaag
      121 gttgctgacg cgttaggaat taatgaatcg caaatttcgc gatggaaaga cagcttcatc
      181 ccaaaaatgg gaatgcttct ggctgttctt gaatggggtg ttgaagacga ggagttggcg
      241 gaactggcta agaaagtagc cagaatgctg acaaaagaaa aagccccgaa gaacggcgaa
      301 ttcttcgagg cctgatgtag aaagactgga tcaatccaca ggagtaatta tgacaaaaca
      361 actcagtcct taccaggaca aaattcacaa acacatacta cgtgatcgct tcctgtccag
      421 cttcaagcag cctggtcgat tccgggctga gttggaaaaa gtgaagctga tgcagaagga
      481 gaaaggtcat gagtaacata tctaatctag ccgaagccag agaggccaga aggctacaac
      541 aaccgcatca aagcagcggt aaggggtatg ccttgctgca ccgtaaaatt atggatgtgc
      601 c
//
LOCUS       DAZGTR010000471          599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGTR010000471 DAZGTR010000000
VERSION     DAZGTR010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.476"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(103..>599)
                     /locus_tag="ACHLCY_005514"
     CDS             complement(103..>599)
                     /locus_tag="ACHLCY_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372489.1"
                     /translation="KNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNAS
                     AWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRR
                     DNVKEIISNLFANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAKYQNEINQLSAPGVN
                     FDDI"
BASE COUNT          171 a          114 c           99 g          215 t
ORIGIN      
        1 accgacaatg acccggacgt aatgatgtaa aaatactgat gttcgtcact gactatttcc
       61 gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa ttttaaatat catcaaaatt
      121 aacacctggc gcagatagtt gattaatttc attttgatat ttcgcctggc gattttgaag
      181 tcttttttca acgttatctt cgagatcctt aaagtctttg tgtttatttt caggatggtc
      241 aacgtcaact ttgttagcga ataaattaga aattatttct tttacattgt cgcgtctaaa
      301 ttttgcatcg tccacttgaa aattatatct tattgctcga cctaaacgtt taaaaacttc
      361 gttaggttct ctcagtcttt ccccgttgag ctcgtttaca acctgatgat cggaacctgt
      421 tgcaaatgat tctgccatga tagctttact gctttcaaac cacgctgaag cattcctggc
      481 tttttcacta caatattggg tgagactatc accattaata cgaattcctc tttgaatgtg
      541 gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca tttccgtttt tatttttaa
//
LOCUS       DAZGTR010000472          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGTR010000472 DAZGTR010000000
VERSION     DAZGTR010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.477"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            50..235
                     /locus_tag="ACHLCY_005515"
     CDS             50..235
                     /locus_tag="ACHLCY_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP0372490.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            476..>596
                     /locus_tag="ACHLCY_005516"
     CDS             476..>596
                     /locus_tag="ACHLCY_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-LEE encoded effector protein NleB"
                     /protein_id="HFP0372491.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYSLEPID"
BASE COUNT          190 a          105 c          111 g          190 t
ORIGIN      
        1 tgattgatat ttttgggccg gagaaacgca gacaggcttt gttgaataaa tcgagctttt
       61 gctgagttta aggatcagat cactcatttt cctgcaaaag ctcattgggc gaaacaatct
      121 gactcagaga acccggatta aacgtctggc ccgcagaacc atctatttct cacgcactgt
      181 agtacgtaat aaaaaagtca tcggtacttt tatcaaaaaa catatattct attaattatt
      241 gccatcacct ttttcttatt gacaacgtta ctctgctgat tgttaaaatc taacctgtat
      301 tcagggttac gattctatac aatagaacag ggaggggaaa ggatgtcatc gttaatgaga
      361 ctttttgtta atgtcaacaa tgctatgaat ttattttaga aaaacaatta agctgacgta
      421 gtgttccgtc taaatgacgg ggcgatatta acatgattag aacaagagga attttatgtt
      481 atcttcatta aatgtccttc aatccagctt cagaggaaag acagctttat caaatagtac
      541 acttctccag aaagtttctt ttgctggaaa agaatattct ctggaaccta ttgatg
//
LOCUS       DAZGTR010000473          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGTR010000473 DAZGTR010000000
VERSION     DAZGTR010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.478"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..596
                     /locus_tag="ACHLCY_005517"
                     /pseudo
     CDS             1..596
                     /locus_tag="ACHLCY_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          189 a           99 c          158 g          150 t
ORIGIN      
        1 tgcaccagcc gtaccggctg ccggggcagc agtatgataa ggagtcgggg ctgtactaca
       61 accggaaccg gtactacgat ccgttgcagg ggcggtatat cactcaggac ccgatagggc
      121 tggagggggg gatggagtct gtatgcgtac ccgctgaatc cggtgaatgg tattgatcca
      181 ttagggttaa gtcccgcaga tgtagcgcta ataagaagaa aagatcaact aaaccatcaa
      241 agagcatggg atatattatc tgatacttat gaagatatga agagattaaa tttaggtggg
      301 actgatcaat ttttccattg tatggcattt tgtcgagtgt ctaaattaaa tgacgctggt
      361 gttagccgat cggcgaaagg gctgggttat gaaaaagaga ttagagatta cgggttaaat
      421 ctgttcggta tgtacggcag aaaagtaaag ctatcccatt ctgaaatgat tgaagataat
      481 aaaaaagact tggctgtaaa tgaccatggg ttgacatgtc catcaacaac agattgctca
      541 gatagatgta gtgattatat taatccagag cataaaaaaa cgataaaggc tttaca
//
LOCUS       DAZGTR010000474          592 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGTR010000474 DAZGTR010000000
VERSION     DAZGTR010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.479"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..174
                     /locus_tag="ACHLCY_005518"
     CDS             <1..174
                     /locus_tag="ACHLCY_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP0372492.1"
                     /translation="NLVPYRVELINRIGQEAVDEIESNHNRHRWTVEECRAIKAKYQQ
                     KLKDLRNSRSEAA"
     gene            171..365
                     /locus_tag="ACHLCY_005519"
     CDS             171..365
                     /locus_tag="ACHLCY_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP0372493.1"
                     /translation="MTFTVKTIPDMLVEAYENQTEVARILNCSRNTVRKYTGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            358..>592
                     /locus_tag="ACHLCY_005520"
     CDS             358..>592
                     /locus_tag="ACHLCY_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0372494.1"
                     /translation="MRDIRQVLECWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLG"
BASE COUNT          171 a          130 c          166 g          125 t
ORIGIN      
        1 aatctcgttc cgtatcgcgt cgaactgatt aatcgcatcg ggcaggaagc agtagacgaa
       61 atcgaatcaa accataaccg ccatcgctgg actgtcgaag agtgcagggc catcaaggcg
      121 aagtatcagc agaaacttaa agacctgcga aacagcagaa gtgaggccgc atgacgttca
      181 cagtaaaaac cattcctgac atgctcgttg aggcatatga aaatcagacc gaggtagcca
      241 gaatactgaa ctgtagtcgc aacacggtca gaaaatacac tggcgataaa gaaggaaaaa
      301 gacacgctat tgtcaacggt gttcttatgg ttcatcgcgg atggggtaaa gatactgatg
      361 cgtgatatcc ggcaggttct tgagtgctgg ggggcatggg cggcaaataa ccatgaggat
      421 gtgacctggt cacccattgc cgccggattt aagggactga tccccgaaaa agtaaaatca
      481 cgcccgcagt gctgtgacga tgatgcgatg gtgatatgcg ggtgtatagc ccgcctttac
      541 cggaacaatc gcgatctgca tgacttgctg gttgattatt acgtgctggg gg
//
LOCUS       DAZGTR010000475          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGTR010000475 DAZGTR010000000
VERSION     DAZGTR010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.480"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          143 a          151 c          154 g          140 t
ORIGIN      
        1 tactataaag ttggtagatt gtgactggct taaaaaatca ttaattaata ataggttatg
       61 tttagagtgt tccccgcgcc agcggggata aaccgcaaga tctgccgcga gtggtttcac
      121 ccggcaggtg ttccccgcgc cagcggggat aaaccggata tttgggcgta ataccgcccg
      181 gatagtgtgt gttccccgcg ccagcgggga taaaccggtg agccattaca gcgtgtacag
      241 gcggtcacgg tgttccccgc gccagcgggg ataaaccgcc gctaatgcgc gtatctcatg
      301 gaaggttgga gtgttccccg cgccagcggg gataaaccgt tcttgcgggt gttgcaaata
      361 ttcttcacgt agagctcccc gcgccagcgg ggataaaccg gagcctgacg agactactga
      421 ggccgttctg tcgagttccc cgcgccagcg gggataaacc accatataac ccgttatctc
      481 tttctcaagt ttttatatta gcagtacttg taataagcaa catatccacg taacacctca
      541 tgttcaaaat agttctccat gccagagagg ttcacaatta tcgataca
//
LOCUS       DAZGTR010000476          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGTR010000476 DAZGTR010000000
VERSION     DAZGTR010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.481"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..258
                     /locus_tag="ACHLCY_005521"
     CDS             37..258
                     /locus_tag="ACHLCY_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372495.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            204..>584
                     /locus_tag="ACHLCY_005522"
     CDS             204..>584
                     /locus_tag="ACHLCY_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0372496.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPEAVLSFHAVFACISLISQDIAKMRLRLMQTDAHGIRRETRRGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRN"
BASE COUNT          130 a          149 c          191 g          114 t
ORIGIN      
        1 aggaggacgg gaggcgtgcg cctcccgtaa caggttatgg caaagatccg atatctgcag
       61 ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg cacagccgtg tgcggaggtg
      121 ctggttcgcc tggggaaggc ggagtacatc acggtgcgac gtccggcagg tcagaaaaag
      181 aaacgtgatg cggagcatgg cgaatgtgga accttttacg gcgaacccga aaaaaccaga
      241 aatcaggacg tgacgtaaga gaggcgggct ggaccagcct gtttcaggcg gtggctgagc
      301 ccttttccgg cgcctggcag cagggcgtga aagccgatcc tgaagccgtc ctctcctttc
      361 atgcggtgtt tgcatgtatt tcgctgatat cccaggatat cgccaaaatg cggctgcgtc
      421 ttatgcagac ggatgcgcat gggatacgca gggaaacgcg ccggggggat attgcccgcc
      481 tctgtcgtcg tcccaacgcc cagcagaacc gcatccagtt ttttgaactg tggctgaacg
      541 ccaaactgcg tcatggcaat acggtggtgc tgaaaatccg taat
//
LOCUS       DAZGTR010000477          583 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGTR010000477 DAZGTR010000000
VERSION     DAZGTR010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.482"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>583)
                     /locus_tag="ACHLCY_005523"
     CDS             complement(<1..>583)
                     /locus_tag="ACHLCY_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4765 family protein"
                     /protein_id="HFP0372497.1"
                     /translation="DALENVVSKCDLSSIFNVNKVLQNTILEFVHEINNISSARWMSK
                     SEKNNKQKEAIEKFKKEVSHMNGGQQFIWGFDKVIQEGLSGLIELSIDINDSTNHRDK
                     SSLSPDGRAVLHFLGTIWNMAMGAVPGYNALSGVSSILHSAIVKESSNICDYIQGAVR
                     IGMDFVPGTRSDLHSRSLQIKYEALKHIEKNIND"
BASE COUNT          189 a          127 c           75 g          192 t
ORIGIN      
        1 atcattaatg tttttttcta tatgcttcaa agcctcatat tttatctgca gcgaacggct
       61 atgtaagtca gagcgagtgc ctggaacaaa gtccattcca atacgtacag ccccctgaat
      121 ataatcacag atattgctag attcttttac tatagcacta tgtaaaatgc tacttacacc
      181 agacaatgca ttataaccag gtacagctcc catcgccata ttccaaattg tacctaaaaa
      241 atgtaacaca gctctcccat caggagaaag agaagactta tcacgatgat ttgtactatc
      301 attaatatcg atacttaact caatcaatcc acttaagcct tcttgaataa ccttatcaaa
      361 cccccaaata aactgctgcc cgccattcat atgggatact tcttttttga acttttctat
      421 tgcctctttt tgtttattat tcttttctga ttttgacatc cagcgagcag atgatatatt
      481 attaatttca tggacaaact caagaatagt attctgaagc actttattaa cattaaagat
      541 agatgataaa tcacattttg acacaacatt ttccaatgca tct
//
LOCUS       DAZGTR010000478          579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGTR010000478 DAZGTR010000000
VERSION     DAZGTR010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.483"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(50..499)
                     /gene="tccP2"
                     /locus_tag="ACHLCY_005524"
                     /pseudo
     CDS             complement(50..499)
                     /gene="tccP2"
                     /locus_tag="ACHLCY_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
BASE COUNT          153 a          100 c          145 g          181 t
ORIGIN      
        1 agagctaata caggtaatta tattataatc acgagcgctt agatgtatta atgccatgct
       61 ctgcaagatg ctgcaccaga cgctgagcca catcaggcag aggtcttgat tgttcatttt
      121 gtactggcgg cgttggcgca ggccagttag gtgccggagg aatatgttca gccatattcc
      181 gggctggttg aatgccatgc tctgcaagat gctgcactag gcgtttggcc acatcaggta
      241 gaggccgatg taaagcgttt ttttctatac ttgactgagt ataagatgtg gaacgttgaa
      301 gtcctgattc cgtataagaa ttttttacct taaaaatagt tgttgcactg gcaggactgt
      361 tgctgggaga aagaggtgag ctggttttta ccgtgttagg tgtgcttttg tgctgtgagg
      421 cagtgaagct actgttacgc gtaacacttg atatactgcg aggaatgctg gaaaaaaaag
      481 aattaatgct atttatcata tagataacct tataagaact ttatgtgata gtctaattta
      541 tccgtttgca tgaaatctac cttttgtgaa attctcgtc
//
LOCUS       DAZGTR010000479          576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGTR010000479 DAZGTR010000000
VERSION     DAZGTR010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.484"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..576
                     /locus_tag="ACHLCY_005525"
                     /pseudo
     CDS             1..576
                     /locus_tag="ACHLCY_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          145 a          141 c          179 g          111 t
ORIGIN      
        1 ggcctacaag attggcgttg gccacccgcc ttccagtgac aacgtggagg cgctggagca
       61 gcagtattat tcccagtgtc tgcagacgct gattgagtcc attgagctgt tactggatga
      121 ggcgctggaa acgggggaaa acgagagcac ggagtttgac gtcaccacgc tgctgagaat
      181 ggacagcgaa cggcgcatga aaacactggg tgaatcggtg aaaaatacgc ttctcacgcc
      241 caatgaggcc cgtaaacgtg agaacctgcc gccccttgcc ggcggtgatg cactgtatct
      301 tcagcagcag aactacagtc tggaggcgct gtcccgtcgt gatgcccgtg aggacccgtt
      361 cgcgtcggcc gggaaaacag tttcagcaca actgcctgac ggcgcatctg acggtaataa
      421 ggcaatcagt gaaacagagc atgatgcagt gaaagcgatg ttcaggggga tactgagaaa
      481 atgacggaac gggaactgtc cattattcgt gcactgggcg aagaattctc cacggtgctg
      541 gcggatttac agcgcacatt tgaggggaag atggcc
//
LOCUS       DAZGTR010000480          572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGTR010000480 DAZGTR010000000
VERSION     DAZGTR010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.485"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            24..>572
                     /locus_tag="ACHLCY_005526"
     CDS             24..>572
                     /locus_tag="ACHLCY_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0372498.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPE"
BASE COUNT          119 a          150 c          166 g          137 t
ORIGIN      
        1 gactgttgtg atgaggccga tatgtgaatc aggtgaacga gagccatagc cgcgcatccg
       61 atatctggcg cgaagtggcc tcgctgtttc gcccacccag ccggttacca gtagcggaag
      121 ccatcaggcg ttatatgcgg gttccacggg gagccaatac ttccggtccg tgggagtcat
      181 cgctgacgcc ctatatgatt gaccccatta atacattatc agcccgtgaa tatgacgcgg
      241 tggtgtttgt gggacctgcg cgaaccggga aaaccgaagg gctgattgat ggctggattg
      301 tgtacggcat catctgtgat ccggcggata tgctggtggt gcagatgact gagacgaagg
      361 cgcgtgagca ttccagaacg cgtctttcca ggacgtttcg ccacagtccg gaggtcagca
      421 agcgcctcag tccttcccgt aatgacaaca acgtccacga taaaatgttt cttgacggct
      481 ccttcctgaa gattggctgg ccgtcgatca ccgtcttttc ctcttcggat taccgtcgtg
      541 tggcgctgac ggattatgac cgtttccctg aa
//
LOCUS       DAZGTR010000481          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGTR010000481 DAZGTR010000000
VERSION     DAZGTR010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.486"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>571
                     /locus_tag="ACHLCY_005527"
     CDS             <1..>571
                     /locus_tag="ACHLCY_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP0372499.1"
                     /translation="VDNGASFEPLSGSLNSVIPPAVQHLTVEVSAADGQYLAQAKWDT
                     PRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYGQQG
                     EPATTTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSETKIADTSQVE
                     TSARYLGTGSQWSVSGPHIKPGKDFWFYVR"
BASE COUNT          117 a          156 c          184 g          114 t
ORIGIN      
        1 gtggataacg gggccagctt tgagccactg tccggttcgc tgaacagcgt catcccgccg
       61 gctgtgcagc acctgacggt ggaggtgagc gcggctgacg gtcagtatct ggcgcaggcg
      121 aaatgggaca cgccgcgggt ggtgaagggt gtgcgcttca gtctgcgcct gaccagcgga
      181 agcggagaag acagccgtct ggtgaccacc gccatcactg cggatacaga gcatcgtttc
      241 agtggtctgc cgctcgggga atacaccctg acagtcaggg cgattaacag ttatggccag
      301 cagggggaac cggccaccac cacgttcagg attaatgcac ctgcggtacc cgccacgatt
      361 gagctgacgc cgggctattt tcagataaca gcggtcccgc gtcttgcggt gtatgacccg
      421 acggtacagt ttgagttctg gttttcggag acaaaaatcg cagacacatc tcaggtggaa
      481 acctctgccc gttatctggg gaccggcagt cagtggagtg tatccggccc gcacattaag
      541 cccgggaagg atttctggtt ttacgtgcgc a
//
LOCUS       DAZGTR010000482          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGTR010000482 DAZGTR010000000
VERSION     DAZGTR010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.487"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>566
                     /gene="espW"
                     /locus_tag="ACHLCY_005528"
     CDS             <1..>566
                     /gene="espW"
                     /locus_tag="ACHLCY_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFP0372500.1"
                     /translation="QIADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFE
                     NISSDDTDKITGRKFSELGEYLYSGNVIKLSQLSIRYLPNISSISLIETKQSLLLHRL
                     YSDEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATF
                     LRKSGAEEKDILACSPRNYGLHHNIKTW"
BASE COUNT          224 a          101 c           99 g          142 t
ORIGIN      
        1 caaattgcag atgaagtaaa atcacttaca tatttgattc cttcagaaaa aaaagagatg
       61 tggatatata aaaacaatgg aaaagataat gcaaaaccaa acttaggaga gcgagacgta
      121 agaatgttcg aaaatattag ttctgatgat acagataaga taacaggaag gaaattttca
      181 gagttaggtg agtatcttta ttcaggaaat gtaataaaac tcagtcagtt atcaattcgt
      241 tacttaccca atatcagctc aatctcatta atagagacaa aacagagttt gttgctacat
      301 cgattgtatt cagatgaagt acttcagaga aatggaacgc ttatcccgac accactacat
      361 gaagaaaaat caattccagc tgacaatata aaaacaatgc tcaacaacat accgacttac
      421 aaaatgttac cgccattcac agaaacacaa ggtaattgtt cttctggcgc agccacgttt
      481 ttacgcaaat caggcgccga agaaaaagat attcttgcat gtagcccccg aaattatggg
      541 ctgcatcata acataaaaac atggga
//
LOCUS       DAZGTR010000483          565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGTR010000483 DAZGTR010000000
VERSION     DAZGTR010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.488"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..543
                     /locus_tag="ACHLCY_005529"
     CDS             <1..543
                     /locus_tag="ACHLCY_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0372501.1"
                     /translation="KFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDPEVLAFIA
                     RKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDENNPEMPPSAVMSPGIEYME
                     DGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAAN
                     RFAVPPPPRLVVNNDNEGNG"
BASE COUNT          149 a          135 c          175 g          106 t
ORIGIN      
        1 aaatttgcgc tggcgctcat gtccgggaaa aacaaaacag cgtcagccat tgccgctggt
       61 tattcggcga agaccgccag ggttaaaggc tcgcagctgg caaaagatcc ggaggtgctt
      121 gcgtttatag cccgtaaaca atgcgagacg gtggaggtgg atgaggttcc tgtttaccgg
      181 cagaaaaaat cagagcagga ggataaaccc cgtcgccgtg aggcggctgc aataccacag
      241 ccggacgaaa acaatccgga gatgccaccg tccgcggtga tgtctcctgg tattgaatat
      301 atggaggatg gtcttcccga tccggtgaaa gcgatggggc gtcttctggt ggagaacatt
      361 aataccgacc ccaggctggc gctggatgcg gcttataagc tggcgcagtt cacgcaccat
      421 aaaaaagggg atgccggtaa aaaatcggca aaaggtgacg cggcgaaaaa agcggctaac
      481 cgttttgcgg tgccaccacc accccgcctg gtggtgaata atgataatga gggcaacgga
      541 tgatacctgt gtggagcacg gcctg
//
LOCUS       DAZGTR010000484          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGTR010000484 DAZGTR010000000
VERSION     DAZGTR010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.489"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          167 a          100 c          130 g          163 t
ORIGIN      
        1 aattaatggt tcttcggaca ttgaaattta tatcgcaaaa attggcaaac tctcctcgac
       61 tactcacttg tgggtatcct ggcgtaaaag gaatgacttg gcaatacccg gtgtgatcat
      121 aagtcattga aaatgatcat ttttatcagt ctttcttcca tgatgaatgc taatgccatt
      181 tgatttgttg aggtgaaaac tgttaaaaat caaaacgatg taattgaaat gaacgttcgg
      241 tagcattcac gctttaaatg tttcttttgt gctgattgga tgaatttggg tcacttctga
      301 tgagagatgt tgcaggaaaa gaagttggca ttgatctatt ggatagttag aattgctgcg
      361 ggtgcttgag gctatctgcc tcaggcatga acaccaaaag gcagatagag aaaagcccca
      421 gttaacatta cgcgtccggc aagacgctta acattaatct gaggccaatt tcatgctttg
      481 cacatgtagg ttagcctctt acgtgccgaa aggcaaggag aagcaggcta tgaagcagca
      541 aaaggcgatg ttaatcgccc
//
LOCUS       DAZGTR010000485          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGTR010000485 DAZGTR010000000
VERSION     DAZGTR010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.490"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            37..258
                     /locus_tag="ACHLCY_005530"
     CDS             37..258
                     /locus_tag="ACHLCY_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372502.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            204..>545
                     /locus_tag="ACHLCY_005531"
     CDS             204..>545
                     /locus_tag="ACHLCY_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0372503.1"
                     /translation="MWNLLRRTRKNQKSGRDVREVGWRSLFQAVAEPFAGAWQQGVKA
                     DPETVLSFHAVFSCISLISQDIAKMRLRLMQTDVQGIRREKRQGDTARLCRRPNAQQN
                     RIQFFELWLNSK"
BASE COUNT          126 a          130 c          179 g          110 t
ORIGIN      
        1 aggagggcgg gaggcgcgag cttcccgcag taacagatgg caaaaatcca gtatctgcag
       61 ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg cacagccgtg tgcggaggtg
      121 ctggttcgcc tggggaaggc ggagtacatc acagcgcgac gtccggcagg tcagaaaaag
      181 aaacgtgatg cggagcatgg cgaatgtgga accttttgcg gcgaacccga aaaaaccaga
      241 aatcaggacg tgacgtaaga gaagtgggct ggcggtccct gtttcaggcg gtggctgagc
      301 cttttgccgg tgcctggcag cagggggtga aagccgatcc ggaaaccgtt ttgtcctttc
      361 acgcggtgtt ttcatgcatt tcgctgatat cccaggatat tgccaaaatg cggcttcgcc
      421 tgatgcagac tgatgtacag ggaatacgcc gggaaaagcg gcagggagat actgcccgtc
      481 tctgtcgtcg tcctaatgcg cagcagaatc gcatccagtt ttttgaactg tggctgaatt
      541 ccaaa
//
LOCUS       DAZGTR010000486          532 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGTR010000486 DAZGTR010000000
VERSION     DAZGTR010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.491"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(1..434)
                     /gene="nleF"
                     /locus_tag="ACHLCY_005532"
                     /pseudo
     CDS             complement(1..434)
                     /gene="nleF"
                     /locus_tag="ACHLCY_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
BASE COUNT          177 a          121 c           61 g          173 t
ORIGIN      
        1 atgcggacat agcagattat aaactttaca tttcgaatct tctttgcctt gtctactttc
       61 ctcaattttt actttcaatg aatgcagaaa aaaagatccc tgatataact atccctgtcc
      121 tctatcgtca ttcccgccct cagcgctccg acaacctcat cagtattttc atttctcacc
      181 tcaaatacac tatttctctt atccatcagc ccacttacaa aagcacttag ctcttgaaaa
      241 atttcctcaa gctcattatt atttctggca catgaatata tagagaccat aacatcaagt
      301 ttatcttgta attcaggaga aagaaagtga ttatgattaa gctcacttac tgattccgga
      361 gtcgaagggt tacctcttcc tgatacatac atccatgaat aaagatttgc tgaagaacca
      421 cttgttggta acatatcaaa acccccttaa caaataaatc cataaatatt attgcaacta
      481 atatatctgg ataaaaatat tatatcttac ttaatactac actaataaga tc
//
LOCUS       DAZGTR010000487          525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGTR010000487 DAZGTR010000000
VERSION     DAZGTR010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.492"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>525
                     /locus_tag="ACHLCY_005533"
     CDS             <1..>525
                     /locus_tag="ACHLCY_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0372504.1"
                     /translation="WSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRI
                     IQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWA
                     LYAIAQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLT
                     FVQDRPSDVVWPYTN"
BASE COUNT          115 a          138 c          171 g          101 t
ORIGIN      
        1 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgattgtg
       61 gggctgcagg tggatgcgga gcagtttggc ggtcagcaga tgacggtgaa ctaccatatc
      121 cgcggtcgca ttattcaggt gccgtcaaac tatgacccgg aaaaacgcac gtacagcggc
      181 atctgggacg gcagcctgaa accggcatac agcaacaacc cggcctggtg tctgtgggac
      241 atgctgactc acccgcgcta cgggatggga aaacgtctgg gggcggcgga tgtggacaaa
      301 tgggcgctgt atgccattgc gcagtactgc gaccagacgg tgccggatgg tttcgggggc
      361 acagagccgc ggatgacctt taatgcatac ctgtcacaac agcgtaaggc gtgggacgtc
      421 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacggcca gacgctgacg
      481 ttcgttcagg accgcccgtc ggatgtggtg tggccgtaca ccaac
//
LOCUS       DAZGTR010000488          507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGTR010000488 DAZGTR010000000
VERSION     DAZGTR010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.493"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..383)
                     /locus_tag="ACHLCY_005534"
     CDS             complement(<1..383)
                     /locus_tag="ACHLCY_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP0372505.1"
                     /translation="MLNGISNAVSTIGLHLRGVASRVGSAGVSGFSVAPQTVRLTPVR
                     FHSPFSPGSSNINARTTFNMSHMSQMSQMSRMSQMATSFTPTRPAPPPPTSGQASGSA
                     GLPPIAQALKDHLAAYELSKASETV"
BASE COUNT          150 a          109 c          104 g          144 t
ORIGIN      
        1 cttacagtct cagacgcttt cgatagttca taggcagcta aatgatcttt taatgcctgt
       61 gcaatgggcg gtagcccggc agacccggat gcctgtccac ttgtcggtgg cggtggtgcc
      121 gggcgagtag gagtaaatga agtcgccatc tgactcatac gactcatctg actcatctga
      181 ctcatatgac tcatattaaa agtcgttctc gcattaatat tcgacgaacc tggagaaaaa
      241 ggggaatgaa atctcaccgg agtaagacgc acagtctgag gggctacaga aaatccagat
      301 acccccgcag agcccactcg acttgcgaca cctctaagat gcagccctat tgtagaaaca
      361 gcgttactaa ttccattaag cataatttat gtctctttat atctaattaa ttggggttat
      421 tattaattca tattaaaact ataactcatt aaattaaatg catatggaaa tatgaatact
      481 tttgtacagc tgtatttatc attctga
//
LOCUS       DAZGTR010000489          505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGTR010000489 DAZGTR010000000
VERSION     DAZGTR010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.494"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>505)
                     /locus_tag="ACHLCY_005535"
     CDS             complement(<1..>505)
                     /locus_tag="ACHLCY_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFP0372506.1"
                     /translation="CACNFLMDKDAQGYIDLSDLDLTSCHFKGDVISKVSFLSSNLQH
                     VTFECKEIGDCNFTTAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQSQL
                     TQSNFRECQIRNSNFDNCYLYASHFTRAEFLSAKEVSFIKSNMTAVMFDHVRISTGNF
                     KDCITEQ"
BASE COUNT          173 a           98 c           77 g          157 t
ORIGIN      
        1 aattgttctg taatgcaatc tttaaaattc cctgtcgata ttcgcacatg atcaaacata
       61 acagctgtca tattcgattt aataaatgat acttctttgg cagacagaaa ctctgctctg
      121 gtgaagtgcg aagcgtaaag ataacaatta tcgaagtttg aatttctaat ctgacattcg
      181 cgaaaattac tctgagtaag ttgactctgc gagaagtcaa ctgtatcaag aatatttttg
      241 ctgaaatcga cacattcacc actcgctttg ataaaaatca cattgtgtaa acgtcgacat
      301 ctaaatatga cattatcaac tattgcagta gtaaaattgc aatccccaat ttctttacat
      361 tcgaatgtta catgttgtag atttgatgat aaaaaagaca ccttcgatat aacgtcacct
      421 ttaaaatgac aacttgttaa atccaaatca gacaggtcga tatacccctg cgcatcttta
      481 tccatcagaa aattgcaagc gcatt
//
LOCUS       DAZGTR010000490          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGTR010000490 DAZGTR010000000
VERSION     DAZGTR010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.495"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..377
                     /locus_tag="ACHLCY_005536"
     CDS             <1..377
                     /locus_tag="ACHLCY_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP0372507.1"
                     /translation="LFCLFSGYHFYRERFAERGFFYKVPAVLRDYLSAIPVKINEKAR
                     YKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLASTLLER
                     ITFYAKKNRDELDKISCKWCCD"
BASE COUNT          170 a           80 c           90 g          160 t
ORIGIN      
        1 cattgttctg tttattcagt ggttatcact tttatcgaga aaggtttgca gaacgcggat
       61 ttttttataa ggttcctgct gtgctaaggg attatttatc agctataccg gtgaaaataa
      121 atgaaaaggc acgatacaaa cccggcatcg ctagctatca caatatcatc acatgtggtt
      181 tttcaacgtt gtcaccctat ataaggcaac aaccattggc aatgcagcaa cgatttaatt
      241 tattatttcc tgattttgtt gatcacatac aattgccgtt accacttgca tcaactcttt
      301 tagagagaat tacattttat gcgaaaaaaa atagagatga gcttgataaa atctcctgca
      361 aatggtgttg tgattaaaag aaaaatttct gatggattga aataaatagt ttctcttaaa
      421 gaaagtatcc ttcttgaaac cagcgcaaaa attcagagca ttgaggtaaa gagagaagaa
      481 aaatttattc aaggttatta
//
LOCUS       DAZGTR010000491          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGTR010000491 DAZGTR010000000
VERSION     DAZGTR010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.496"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>490
                     /locus_tag="ACHLCY_005537"
     CDS             <1..>490
                     /locus_tag="ACHLCY_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372508.1"
                     /translation="KLMHYSVFTDKTHQIIKQKYFNDIVPMILALDIRNTYRPDNEMA
                     FYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPANAHFKDIFDGVYEFIRN
                     IRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLDKVQVACYYLTILLAFE
                     RRT"
BASE COUNT          180 a           91 c           74 g          145 t
ORIGIN      
        1 aagctaatgc actattctgt ttttacagac aaaacacatc aaataataaa acaaaaatat
       61 tttaatgaca ttgtaccaat gattctcgca ctcgacatca ggaacacata tcgcccggat
      121 aatgagatgg cattttacta tcatattcat tcttttctca ctcagatacc ggataatgag
      181 gatgatatat atcatgctgc aaggacatat ctgcgaaatt acgttaagtt atgtttatcc
      241 ggatacacgc cagcgaatgc gcatttcaaa gatatctttg atggcgtata tgaattcatt
      301 cgtaatattc gcaaaaacag tacaccagga aaaacaaaac ttatcgcaac tatcaacaca
      361 tgcaaagaaa cctgtaaaca tctgctttat ttaagtaatg aagacaagga aaaaataatt
      421 tctgacttag ataaagttca ggttgcatgt tattatctca ctatattact ggctttcgaa
      481 agacgaactt
//
LOCUS       DAZGTR010000492          488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGTR010000492 DAZGTR010000000
VERSION     DAZGTR010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.497"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>488)
                     /locus_tag="ACHLCY_005538"
     CDS             complement(<1..>488)
                     /locus_tag="ACHLCY_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0372509.1"
                     /translation="ATAILRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYL
                     ASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSSWVLEYAAGNGAALL
                     QKGGQIRCRFKVSGALAANQYVMAFYWPVSSLPQGVALTGDGGNNLLAAFYIQTDAKD
                     LNV"
BASE COUNT           87 a          169 c          120 g          112 t
ORIGIN      
        1 acattcaggt cttttgcatc tgtctggatg tagaacgctg ccagcaggtt attccccccg
       61 tctccggtca gggcaacgcc ctgtggcagt gaagataccg gccagtaaaa cgccataaca
      121 tactggttcg cagccagcgc tcccgaaacc ttaaagcggc agcgaatctg cccccctttc
      181 tgtaacagag ccgcaccgtt gcccgcggcg tactccagca cccagctgct tttaccggct
      241 tccttggtca gcttcactgc cttacctccg gttccctccg catcgctgac cacttctgcc
      301 ctgccgccac tggctgacca tccctgtact ttcaggcttc cctctgactc gctggcaagg
      361 taagagagca gtgttgtgac gcctgtggct tctgcaccgg aaggcgatga cgggcgcacc
      421 tctgatactg tcgatgatgc ccccgcgttt agcgccactc ttcccgcatg gcgcaaaatc
      481 gccgttgc
//
LOCUS       DAZGTR010000493          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGTR010000493 DAZGTR010000000
VERSION     DAZGTR010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.498"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>485
                     /locus_tag="ACHLCY_005539"
     rRNA            <1..>485
                     /locus_tag="ACHLCY_005539"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          121 a          110 c          163 g           91 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaacc
//
LOCUS       DAZGTR010000494          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGTR010000494 DAZGTR010000000
VERSION     DAZGTR010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.499"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>484
                     /locus_tag="ACHLCY_005540"
     CDS             <1..>484
                     /locus_tag="ACHLCY_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0372510.1"
                     /translation="RYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRY
                     EYNIAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGS
                     HTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWYYDEADRLTHRTV
                     K"
BASE COUNT          120 a          146 c          145 g           73 t
ORIGIN      
        1 cgctatgacc atgaccgttt tggtcaggtg acggcggtgc accgcgagga aggcctgagc
       61 cagtaccacg catacgacag ccgtggacag ttaactgcgg tgaaagacac gcagggccat
      121 gaaacgcggt atgaatacaa catcgccggt gacctgaccg ccgtcattgc cccggacggc
      181 agcagaaacg ggacacagta cgatgcgtgg ggaaaggcca tctgtaccac gcagggcggg
      241 ctgacgcgca gtatggaata cgatgctgcc ggacgggtca tccgcctgac cagtgaaaac
      301 ggcagccaca ccaccttccg ttacgatgta ctcgaccggc tgatacagga aaccggcttt
      361 gacggccgca cacagcgtta tcaccacgac ctgaccggca aacttatccg cagcgaggat
      421 gaggggctgg tcacccactg gtactatgac gaagcagacc gcctcactca ccgcaccgtg
      481 aagg
//
LOCUS       DAZGTR010000495          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGTR010000495 DAZGTR010000000
VERSION     DAZGTR010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.500"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>484)
                     /locus_tag="ACHLCY_005541"
     CDS             complement(<1..>484)
                     /locus_tag="ACHLCY_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFP0372511.1"
                     /translation="YYKLINYPIMMVHDEWLGDLTGVNQVSFRRLRETSSTRNQLNKI
                     LRQEIQDKISGVELSDINKEGFLYQSIGKIRLLALSSALFDIQCPDYIFSRLYRETLI
                     REIGYQNVKQLSFYWQGGQCKPEYGEERFCAELIKYGAGNLEWLFADNPLWTIVKYLL
                     P"
BASE COUNT          158 a          101 c           66 g          159 t
ORIGIN      
        1 cggtaaaaga tatttgacta tggtccataa aggattatca gcaaatagcc actccaaatt
       61 tcctgcaccg tattttatta actcagcaca aaaccgttct tcaccatatt ctggtttaca
      121 ttgccctccc tgccaataga atgataactg cttcacattt tgatatccta tctctctaat
      181 cagagtttct cgatataatc gtgagaaaat ataatcaggg cattgaatgt caaataatgc
      241 actacttaga gcaagcaatc ttattttccc aattgactga tataaaaaac cttccttatt
      301 tatgtcagat aattccacac cagaaatttt atcttgtatt tcctgacgca atatcttatt
      361 gagttgatta cgagtagaac tagtttctct taaacgccga aaactgacct gattgacacc
      421 ggttaaatca cctagccatt catcatgaac catcatgatt ggataattaa ttaatttata
      481 gtag
//
LOCUS       DAZGTR010000496          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGTR010000496 DAZGTR010000000
VERSION     DAZGTR010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.501"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>483
                     /locus_tag="ACHLCY_005542"
     CDS             <1..>483
                     /locus_tag="ACHLCY_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0372512.1"
                     /translation="QRDDWLKREIQRVYDENHQVYGVRKVWRQLLREGIRVARCTVAR
                     LMAVMGLAGVLRGKKVRTTVSRKAVSAGDRVNRQFVAERPDQLWVADFTYVSTWQGFV
                     YVAFIIDVFAGCIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYISLAY
                     T"
BASE COUNT          100 a          113 c          154 g          116 t
ORIGIN      
        1 cagcgcgatg actggctgaa gagagagata cagcgcgtat acgatgaaaa tcatcaggtg
       61 tacggtgtgc gtaaagtctg gcgccagttg ttacgcgaag gtatcagggt ggccagatgt
      121 acagtggcgc gcctcatggc ggttatggga cttgccggtg ttctccgggg taaaaaggtc
      181 cgtactaccg tcagccggaa agccgtttcc gcaggcgacc gcgtaaaccg tcagttcgtg
      241 gcagaacgtc ctgaccagct gtgggtggct gattttactt acgtcagcac atggcagggc
      301 ttcgtctatg tggcgttcat cattgatgtg tttgccggat gtatcgtggg gtggcgagtc
      361 tcatcgtcta tggaaacgac attcgtgctg gatgcactgg agcaggcgtt gtgggcccgt
      421 cggccgtccg gcacagtcca tcacagtgat aaaggttctc agtatatatc actggcctat
      481 acg
//
LOCUS       DAZGTR010000497          481 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGTR010000497 DAZGTR010000000
VERSION     DAZGTR010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.502"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..270
                     /locus_tag="ACHLCY_005543"
                     /pseudo
     CDS             1..270
                     /locus_tag="ACHLCY_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          192 a           97 c           63 g          129 t
ORIGIN      
        1 atataattga aataggctac aatataataa acaccgatct ggtcattggc gaaaaaattc
       61 atttcaggac actaagcaat cgttctttat attttaacct cacccaaccc ccattaatcg
      121 ccacaataaa agatgatgtg ttttgcactc ttcatgatta ctataaccac aataatgaaa
      181 cgaaatccac tattgacaat tacatatcaa aaataaagga taatcataat acccccatgg
      241 ttattaaaca acgaaaatgt tcaggaataa aaaaaaagag acaattcccg agataaaaca
      301 aaagagcata aacatatgaa ccggaaattc tatttcaaaa atatcctgtg gggaatgtgc
      361 cccattataa tatccatatt gcatctgata tatgacacac caacagaagc atcgataaaa
      421 gttgacttat ttctgatact cagtggcctg gcccttcctt gttcaatata cctgacaaaa
      481 a
//
LOCUS       DAZGTR010000498          479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGTR010000498 DAZGTR010000000
VERSION     DAZGTR010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.503"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          150 a           73 c           95 g          161 t
ORIGIN      
        1 ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc
       61 ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa
      121 aaggcagtga tagtatcgta aagggaaacg ttaacaacta tcaaaaagcg ctcattttaa
      181 aaagttattt tgttctccga tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt
      241 aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact gaaatctgcc
      301 ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata aaatgtcaga
      361 gaagcatgat tacgtggtta ttgaaccgcc ccgggtttcc tggagagtgt tttatctgtg
      421 aactcaggct gccagatcat cgtttccgat ggaagcataa taagcttttt ctgcttctg
//
LOCUS       DAZGTR010000499          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGTR010000499 DAZGTR010000000
VERSION     DAZGTR010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.504"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>473
                     /locus_tag="ACHLCY_005544"
     CDS             <1..>473
                     /locus_tag="ACHLCY_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="simA domain protein"
                     /protein_id="HFP0372513.1"
                     /translation="VIMRKLLLPLLFMAGTVNAASSVKEICTDYTKYLGHVYGFAVSQ
                     DESMRKKLLSDMKRLKLSEAMVQQELYKVATNENAKYQYSRLLNPDANEINRSTFDYM
                     VKACETAPDFAIPSWGVLVASNAVNKEDVGRNGIDSIRNAPGMRHQNVQGTLEER"
BASE COUNT          153 a           92 c          109 g          119 t
ORIGIN      
        1 ggttataatg agaaaactat tactaccgtt attatttatg gctgggactg ttaatgcagc
       61 atcaagcgtg aaggagattt gtaccgatta tacgaaatac cttgggcacg tttacggctt
      121 tgctgtcagt caagacgaat ccatgcgcaa gaagttactg tcagatatga aacgtcttaa
      181 actttctgaa gcgatggtgc agcaagaact atataaagtc gcaaccaacg aaaatgctaa
      241 atatcaatat tctcgcctat taaatcccga cgcaaacgag atcaatcgaa gcactttcga
      301 ttatatggta aaggcatgcg aaaccgctcc tgattttgct atccctagct ggggtgtgct
      361 ggtggcgagc aatgccgtta ataaagaaga tgttggaaga aatggcattg actcaataag
      421 aaatgcccca ggaatgcgcc atcaaaacgt acagggtacg cttgaagaac gag
//
LOCUS       DAZGTR010000500          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGTR010000500 DAZGTR010000000
VERSION     DAZGTR010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.505"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..430
                     /locus_tag="ACHLCY_005545"
     CDS             <1..430
                     /locus_tag="ACHLCY_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0372514.1"
                     /translation="ISIANLSGRLVLGILSDKIARIRVITIGQVISLVGMAALLFAPL
                     NAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGSICGSIIA
                     SLFGGFYVTFYVIFALLILSLALSTTIRQPEQKVLREAHI"
BASE COUNT           93 a          108 c          120 g          152 t
ORIGIN      
        1 tatttccatc gccaaccttt caggtcgtct ggtgctgggt attctgtctg acaaaatcgc
       61 ccgtatccgt gttatcacca ttggtcaggt gatatcgctg gtgggtatgg cggccctgct
      121 gtttgcacca ttgaatgcag tgacgttctt tgcagcgatt gcctgcgtgg catttaactt
      181 tggcggcact attaccgtct ttccgtcact ggtcagtgag ttctttggcc tcaataacct
      241 ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc ggtagcattt gtggttcgat
      301 tatcgcctca ctgtttggcg gcttctatgt gaccttctac gtgattttcg ccctgctgat
      361 tctgtcattg gcgctttcta cgacaattcg tcagccagag cagaaagtat tgcgtgaggc
      421 gcatatttag aaatatgttg ctactgaaat aaatgcgagg agggagactt caa
//
LOCUS       DAZGTR010000501          462 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGTR010000501 DAZGTR010000000
VERSION     DAZGTR010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.506"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>462)
                     /locus_tag="ACHLCY_005546"
     CDS             complement(<1..>462)
                     /locus_tag="ACHLCY_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372515.1"
                     /translation="NEILKSDSPDIRCRNIDFVFGNLSEELYQRLKNNNTLDEFIKSV
                     FDSYSNEYANSGVAALLQYCSSKMDLPSNNDTYHEMYHALNMNLSSKNFEDGHISTGT
                     IFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLSPHRLIKVLELFNASQSKA"
BASE COUNT          153 a           84 c           74 g          151 t
ORIGIN      
        1 gctttactct gactggcgtt aaaaagctcc agaactttaa tgagcctgtg cggacttaat
       61 cttacatggt gagggtcgtt accctgagta ggaaccaaat cacatgccgc agatacacat
      121 aaataccatt tgttcgactc tgtatcaaag aaaatagtgc cagtagaaat atgaccatct
      181 tcaaaattct ttgaagacaa attcatattt aaagcatgat acatttcgtg ataagtatca
      241 ttatttgatg gcagatccat ttttgaagag caatattgga gcaatgcagc aactccgctg
      301 ttagcgtatt catttgaata gctatcaaaa acacttttga taaattcatc cagcgtatta
      361 ttatttttaa gtctttgata aagctcttct gataaattac caaatacaaa gtcaatattt
      421 ctacatctaa tatcaggcga gtctgatttt aatatctcat tt
//
LOCUS       DAZGTR010000502          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGTR010000502 DAZGTR010000000
VERSION     DAZGTR010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.507"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>450
                     /locus_tag="ACHLCY_005547"
     CDS             <1..>450
                     /locus_tag="ACHLCY_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0372516.1"
                     /translation="GFPPSHAGTITVYEDSQPGTLNDFLGAMSEDDVRPEALRRFELM
                     VEEVARHAEEAKKNAGEAETSARNAGISVSQAEASAANADTSAGEASESARQAAESAA
                     SAKKSEDASSSSASAAAQKASESSQSAAEAELSRKTAESAAGNAARDA"
BASE COUNT          116 a          111 c          151 g           72 t
ORIGIN      
        1 ggattcccgc cgtcacatgc cgggaccatc accgtgtatg aagattctca accggggacg
       61 ctgaatgatt ttctcggtgc catgtcggag gatgacgtcc gtccggaggc actgcgtcgt
      121 tttgaactga tggtggaaga ggtggcgcgt cacgcagagg aggcgaagaa gaatgccgga
      181 gaagcagaga cgtccgcgag gaatgccggc atatcagtca gtcaggcgga agcgagcgcg
      241 gcaaatgctg acacttcagc aggggaggca tcggagtcag cccggcaggc ggcagaaagt
      301 gcagcctcag caaagaagtc agaggatgcg tcctcgtcct cggcttctgc ggccgctcaa
      361 aaagccagtg agtcatcaca aagtgcagca gaagctgaat tgtcaagaaa gacggcagaa
      421 agtgcagccg gtaatgcagc cagggatgca
//
LOCUS       DAZGTR010000503          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGTR010000503 DAZGTR010000000
VERSION     DAZGTR010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.508"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>448
                     /gene="escU"
                     /locus_tag="ACHLCY_005548"
     CDS             <1..>448
                     /gene="escU"
                     /locus_tag="ACHLCY_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFP0372517.1"
                     /translation="YFMGHSYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLF
                     SVFDFWFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKK
                     STVIVKNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELY"
BASE COUNT          140 a           78 c           94 g          136 t
ORIGIN      
        1 tttattttat ggggcattca tatgcaaatg agtttgctaa tttcacagga ctgaacgcat
       61 atcaagctct tgtcgttgtt gccttttttg tttttctttt atggaaaggc gtgctattcg
      121 gatatctact cttttcagta tttgatttct ggttccagaa gcatgaggga ctgaagaaaa
      181 tgaaaatgag taaagatgag gtgaaacgag aagccaagga tactgatggt aaccctgaaa
      241 ttaaagggga gcgccgtcgc cttcattccg agatacaaag tggaagtttg gctaataaca
      301 tcaaaaaatc aaccgttatt gttaaaaacc cgactcacat tgcgatttgc ctatactata
      361 aacttgggga gactccatta cctttagtta ttgaaacagg aaaagatgcc aaagctctac
      421 agatcattaa actggctgaa ctctatga
//
LOCUS       DAZGTR010000504          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGTR010000504 DAZGTR010000000
VERSION     DAZGTR010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.509"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>445
                     /gene="yfdF"
                     /locus_tag="ACHLCY_005549"
     CDS             <1..>445
                     /gene="yfdF"
                     /locus_tag="ACHLCY_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFP0372518.1"
                     /translation="LKNLFNGKEGISTCIKHLLELIKNAIRVNDDPYRFNINNSSVTY
                     IDIGSNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHDINNKEMIFSAL
                     KEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDK"
BASE COUNT          173 a           78 c           76 g          118 t
ORIGIN      
        1 agctcaaaaa cctttttaat ggtaaggaag gaataagcac ctgtattaaa catctacttg
       61 agcttataaa aaacgccata cgagtaaacg acgatcctta tagatttaat attaataatt
      121 cctcagttac ttatattgat attggctcca atgatacaga ccatattact attggtatcg
      181 acaaccaaga accaatagaa ttacctgcga actataaaga caaagaactc gtccgtacta
      241 tcattaatga caacatagtt gagaagactc atgatatcaa taacaaggaa atgatcttca
      301 gcgcattaaa agaaatatat gatggagatc ctggttttat cttcgataag atatcacaca
      361 aactcagaca tacggtaacg gagtttgatg agagcgggaa tagcgaacca acggacttat
      421 ttacctggta cggtaaagat aaaaa
//
LOCUS       DAZGTR010000505          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGTR010000505 DAZGTR010000000
VERSION     DAZGTR010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.510"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            1..443
                     /locus_tag="ACHLCY_005550"
                     /pseudo
     CDS             1..443
                     /locus_tag="ACHLCY_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          109 a          109 c          143 g           82 t
ORIGIN      
        1 ggggaaaacg agagcacgga gtttgacgtc accacgctgc tgagaatgga cagcgaacgt
       61 cgcatgaaaa cgctgggtga atcggtgaaa aatacgcttc tcacgcccaa tgaggcccgt
      121 aaacgtgaga acctgccgcc ccttgccggc ggtgatgcac tgtatcttca gcagcagaac
      181 tacagtctgg aggcgctgtc ccgtcgtgat gcccgtgagg atccgttcgc gtcttccggg
      241 aaaacgtcgt caggcacacg ccaggagggc gcgtctgacg gtaataaggc cataagcgaa
      301 acagagcgtg atgcggtgaa ggcgatgttc agggggatac tgcgaaaatg acagagcgtg
      361 aactgtccat tatccgtgcg ctgggtgaag aattctccac ggtgctggcg gatttacagc
      421 gcacatttga ggggaagatg gcc
//
LOCUS       DAZGTR010000506          436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGTR010000506 DAZGTR010000000
VERSION     DAZGTR010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.511"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..101)
                     /locus_tag="ACHLCY_005551"
     CDS             complement(<1..101)
                     /locus_tag="ACHLCY_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFP0372519.1"
                     /translation="MIPVKRERMLTIRVTDEEHARLLARCEGKQLAS"
     gene            145..336
                     /locus_tag="ACHLCY_005552"
     CDS             145..336
                     /locus_tag="ACHLCY_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP0372520.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT          106 a          135 c          116 g           79 t
ORIGIN      
        1 catgacgcaa gttgttttcc ctcacaacgt gccagcagcc gcgcatgttc ctcatccgtg
       61 acccgtatcg tgagcatcct ttcgcgtttc accggtatca ttaaaaacct ccgacagact
      121 ccccacacat ggagaaacag aactgtgact aaacaggaaa aaaccgccct taacatggcc
      181 cgcttcatca gaagccagac gctgaccctg ctggaaaaac tgaatgaact ggacgccgac
      241 gaccaggctg acatctgcga agcgcttcac gaccacgctg acgagcttta ccgcagctgc
      301 ctcgcacgct tcggggatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
      361 acagctggcc tgtgagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      421 tttagcgggt gtcggg
//
LOCUS       DAZGTR010000507          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGTR010000507 DAZGTR010000000
VERSION     DAZGTR010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.512"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            229..348
                     /locus_tag="ACHLCY_005553"
                     /pseudo
     CDS             229..348
                     /locus_tag="ACHLCY_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          108 a          119 c           69 g          134 t
ORIGIN      
        1 tccctaatct cacataggat cagatctcgt ctgatcgttc cccctgcgct cactaccatg
       61 gctctttacc acagcagccg gggttgtttg ggaccgactt ccgacagcag atcccggtgg
      121 gcctgccacc atctcttcac aagcttatgc ttattacatg caaaacgtcc cgttttgcat
      181 ctttgtgcct acggcacacc gatgatcttt ccctgtgatc tttccctgaa tctgcccctg
      241 agaacctgaa tcaaacgtct gactcgcaga accatctgtt tctcgcgctc tgtagaactt
      301 catgaaaaag tcatcggtgc ttttatcgaa aaatacatgt tctactaatt gaagtcatca
      361 tcaaaaaatt taattaatcg aatcatacct ataacctttt aattttactt ttttgtcttc
      421 aacattaaac
//
LOCUS       DAZGTR010000508          428 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGTR010000508 DAZGTR010000000
VERSION     DAZGTR010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.513"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>428)
                     /locus_tag="ACHLCY_005554"
     CDS             complement(<1..>428)
                     /locus_tag="ACHLCY_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0372521.1"
                     /translation="GRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWL
                     GWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKT"
BASE COUNT           87 a          171 c           89 g           81 t
ORIGIN      
        1 gttttgatgg ccccgcccag cttccagccc tggcttatcc ccaccacact ctcatcgggg
       61 atcccggctt ccgccagtga atccagaatc tgccccacac ctgacgggtc aataccgata
      121 tgatccagta actcagcctc atgaatacga cgcacatact ccgccacttc cgccgtgtca
      181 tccccgaccc gacggacaat cgtcatgtct ccacaggcca caaaatcctg aaaccgggat
      241 gcctcactct tccgtctgac caccgcggtt tcatgcaccc aggcatggcc ccagcccagc
      301 cattcgcggg tttccctgtc acggccagtc acgtacattc ccagcagatc atccaggccc
      361 ccgccgtcaa tccccaccgt caccacatca gcgcgctgca ggatatcgtc caggctgacg
      421 cgcctgcc
//
LOCUS       DAZGTR010000509          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGTR010000509 DAZGTR010000000
VERSION     DAZGTR010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.514"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>427)
                     /locus_tag="ACHLCY_005555"
     CDS             complement(<1..>427)
                     /locus_tag="ACHLCY_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0372522.1"
                     /translation="RITPDRNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGL
                     AATQGHHIQENSTSFFRNGGRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAI
                     LSNGAKYNPTTFSPVDAQTVEQLKMTAEIVCSVFRVPAYK"
BASE COUNT           82 a          135 c          107 g          103 t
ORIGIN      
        1 tcttgtaggc cgggacacgg aacaccgaac agacaatttc agcggtcatc ttcagttgtt
       61 ccaccgtctg cgcatccaca ggtgaaaacg tcgtggggtt gtattttgcc ccgttgctca
      121 gaatggccgt tttccccgca ttttcgcctg tatacccgct gtcccagttg ctcttcagtt
      181 ttttcgcatt ttcttccgtt atactgccgg ggatctcaat caccccggac ggcctgccgc
      241 catttctgaa aaaagacgtc gaattttcct gaatatgatg cccctgcgtg gccgccagcc
      301 cggcggcata caccggcggc agccccacaa gcggatgaaa aaaacagtta aaccggtcgt
      361 ggatcacttc ccgggcaggc accgtcaccg cctccgtgat cccgcagttc cggtccggcg
      421 tgatgcg
//
LOCUS       DAZGTR010000510          424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGTR010000510 DAZGTR010000000
VERSION     DAZGTR010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.515"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..341)
                     /locus_tag="ACHLCY_005556"
     CDS             complement(<1..341)
                     /locus_tag="ACHLCY_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0372523.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVL"
BASE COUNT          117 a          108 c           84 g          115 t
ORIGIN      
        1 tccagtacct gaactatcgc tttcaccacg acagaaaagg accgcctgac atcactgact
       61 ggcacaagct gaccagtttc ctgctcaaat ttcagtcggt cacgttctga ctggtaccat
      121 gctttacgcg catgaggatc catttcctcg ttatctacag gcagaggagc tttcatcagc
      181 tcggcaagga tatctgtcag tcggtacagt ttgagattgc tctcatgacc accggctggg
      241 cttatgtttt ttacccgagc cgcaacagtc tgtcgatgag caccggataa tgcggccagt
      301 tgggaaatat tcagatgcag attttttaat tcacgatcca taactccccc tgaaaattat
      361 gtaaacacac accagtgatg aacaaaaaac aaccagattc gacactaaaa atttttattt
      421 ttct
//
LOCUS       DAZGTR010000511          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGTR010000511 DAZGTR010000000
VERSION     DAZGTR010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.516"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..228)
                     /locus_tag="ACHLCY_005557"
     CDS             complement(<1..228)
                     /locus_tag="ACHLCY_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0372524.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQ"
     gene            complement(218..>422)
                     /locus_tag="ACHLCY_005558"
     CDS             complement(218..>422)
                     /locus_tag="ACHLCY_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP0372525.1"
                     /translation="VLGKKIINLYEIDDLCAKRIISLLPESEKNIEIRVNGALTGYGE
                     LVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT          141 a           90 c           60 g          131 t
ORIGIN      
        1 ctgactgtta ttatatattt ccttcccaac cggcatcatt acgaacattg acaatagcaa
       61 agcaacccca ttaagtgtca tatttgatgg cacttgctga agtcccagcg cgtttcggac
      121 aataacaaaa acaatcgaga atttaataaa acatgtcccg gatgctatta taaacggcaa
      181 tagtgtaaat aatgataata tcgctatcag cgaaatgcta tttgacatta ttattccctg
      241 ataaccaaga atgaatttct acgcccaatt tatcatccac ttcaacaagt tcgccatagc
      301 cagttagcgc accatttaca cgtatctcta tatttttctc agattcagga agcagagata
      361 ttattctttt tgcacagaga tcgtctattt cataaagatt aattattttc ttaccaagaa
      421 ca
//
LOCUS       DAZGTR010000512          422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGTR010000512 DAZGTR010000000
VERSION     DAZGTR010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.517"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(64..255)
                     /locus_tag="ACHLCY_005559"
     CDS             complement(64..255)
                     /locus_tag="ACHLCY_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0372526.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(252..>422)
                     /gene="dicB"
                     /locus_tag="ACHLCY_005560"
     CDS             complement(252..>422)
                     /gene="dicB"
                     /locus_tag="ACHLCY_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0372527.1"
                     /translation="NVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKICILSML
                     ARLRPIQKGCWQ"
BASE COUNT          120 a           89 c           83 g          130 t
ORIGIN      
        1 tgaacaaatc cctgccattg ctggcatata agaatgaaat cggatgttta ttacggaact
       61 gttttaaaga cctgccggga tttcgttatt atcctggtga ataactttat cgaccgggta
      121 acagttaccg ggaattttct gttcggttgc tgcagtcaca cactcctgca ttgtcctgtg
      181 aacactgact gcaatatcaa ctggctctcc ggaaacaaga aaaactgtca gaacaagtgc
      241 aaatgctgta ttcattgcca gcatcctttt tgtatcggac gtaaacgggc cagcattgaa
      301 agaatgcata ttttatttaa tagctcccgt tcgtgttttc tcttgttaat ggcatcttca
      361 gtaaatacag ggttactgat agtgacacca atttcaaaac aaccttcaga cgtattaacg
      421 tt
//
LOCUS       DAZGTR010000513          405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGTR010000513 DAZGTR010000000
VERSION     DAZGTR010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.518"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>405
                     /locus_tag="ACHLCY_005561"
     rRNA            <1..>405
                     /locus_tag="ACHLCY_005561"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          106 a           97 c          124 g           78 t
ORIGIN      
        1 gcggtccggc cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg
       61 acgtcaagtc atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca
      121 aagagaagcg acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg
      181 agtctgcaac tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg
      241 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa
      301 agaagtaggt agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt
      361 gaagtcgtaa caaggtaacc gtaggggaac ctgcggttgg atcac
//
LOCUS       DAZGTR010000514          399 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGTR010000514 DAZGTR010000000
VERSION     DAZGTR010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.519"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..163)
                     /locus_tag="ACHLCY_005562"
     CDS             complement(<1..163)
                     /locus_tag="ACHLCY_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0372528.1"
                     /translation="MLHDHLAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTP"
BASE COUNT          139 a          101 c           54 g          105 t
ORIGIN      
        1 ccggagtccg gcgcgcctta cgcaaacact ctgctcgttt ctgtgccgcc acttttcttt
       61 tctggtcatc acttagctgt accatcactt ttgcccatcg ttcagctgct ctccggtaca
      121 gtcctttttt ctccagacat tctgccaggt gatcatgtag cataagtaac ctccgtttat
      181 ctacagactg ccatcctgaa ttaaccgtcc ttaataagac aacaataaaa aactaattat
      241 ataaaaacaa taaaaataat atacaaaaaa aactaaacaa caaagaaaag caaatcatat
      301 ctggcatttt aattgaaaga gcaattaccg aacaaaagac gctgactata tactcaaaac
      361 caaacaacaa ttctgccaat caggtatcat ggcaacaca
//
LOCUS       DAZGTR010000515          394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGTR010000515 DAZGTR010000000
VERSION     DAZGTR010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.520"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>394
                     /locus_tag="ACHLCY_005563"
     CDS             <1..>394
                     /locus_tag="ACHLCY_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001721311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HFP0372529.1"
                     /translation="KAKRSELSALSQLSIEKKSKILPLLEIEPVPIDPDSGIALKSYN
                     ETLIEFGKKVSKSCSDMQGVYIDGLLIEEHFISPEDHYPIINAVNQVRDMEIRVIPVS
                     SPTRPSNYKRAIDELMQNEICLRLTTLDL"
BASE COUNT          136 a           65 c           73 g          120 t
ORIGIN      
        1 aaagcgaaac gttctgagtt atcagcttta tcgcagctat ccattgaaaa gaaatcaaaa
       61 attttgccat tactcgaaat tgaaccagtg ccaattgacc ctgattcagg tattgcctta
      121 aagagttata acgagactct tatagagttc ggaaagaaag tctcaaaatc ttgctcagat
      181 atgcaaggtg tttatattga tggattatta attgaagagc attttatttc tcctgaagat
      241 cattacccta taataaatgc ggttaatcaa gttagagata tggagataag agttattcct
      301 gtcagttcac caactcgccc atctaactat aaaagagcga ttgatgaatt aatgcagaat
      361 gaaatatgct tgagattaac cacgttagat ctgg
//
LOCUS       DAZGTR010000516          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGTR010000516 DAZGTR010000000
VERSION     DAZGTR010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.521"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>391
                     /locus_tag="ACHLCY_005564"
     rRNA            <1..>391
                     /locus_tag="ACHLCY_005564"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          102 a           88 c          129 g           72 t
ORIGIN      
        1 gagcactgtt tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac
       61 cggagaatgt tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa
      121 caacccagac cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag
      181 gcccagacag ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct
      241 cactggtcga gtcggcctgc gcggaagatg taacggggct aaaccatgca ccgaagctgc
      301 ggcagcgacg ctgatgcgtt gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc
      361 tgtgaggcat gctggaggta tcagaagtgc g
//
LOCUS       DAZGTR010000517          380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGTR010000517 DAZGTR010000000
VERSION     DAZGTR010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.522"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>380
                     /locus_tag="ACHLCY_005565"
     CDS             <1..>380
                     /locus_tag="ACHLCY_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0372530.1"
                     /translation="TYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPA
                     VPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGG
                     FNSETVQDVMLGAVERRFGNDLPSS"
BASE COUNT           84 a           93 c          122 g           81 t
ORIGIN      
        1 acgtatggtt atcgtcgggt atgggcgctg cttcgcagac aggcagaact tgatggtatg
       61 cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct gttgcttgag
      121 cgaaaacctg ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc cgtgaaagaa
      181 agcaatcagc gatggtgctc tgacgggttc gagttctgct gtgataacgg agagagactg
      241 cgtgtcacgt tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc ggtgactacc
      301 ggcggcttca acagtgaaac agtacaggac gtcatgctgg gagcggtgga acgccgcttc
      361 ggcaacgatc ttccgtcgtc
//
LOCUS       DAZGTR010000518          378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGTR010000518 DAZGTR010000000
VERSION     DAZGTR010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.523"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>378
                     /locus_tag="ACHLCY_005566"
     CDS             <1..>378
                     /locus_tag="ACHLCY_005566"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000707060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0372531.1"
                     /translation="GRLVEAKDYPNLLNAINHLILSKTSNCNDFILLIAGDGALRNKL
                     LDLVCQLNLVDKVFFLGQRSDIKELMCAADLFVLSSEWEGFGLVVAEAMACERPVVAT
                     DSGGVKEVVGPHNDVIPVSNHIL"
BASE COUNT          104 a           49 c           86 g          139 t
ORIGIN      
        1 taggaagact tgttgaagca aaagactatc cgaacttatt aaatgcaata aatcatttga
       61 ttctttcaaa aacatcaaat tgtaatgatt ttattttgct tattgctggc gatggcgcat
      121 taagaaataa attattggat ttggtttgtc aattgaatct tgtggataaa gttttcttct
      181 tggggcaaag aagtgatatt aaagaattaa tgtgtgctgc agatcttttt gttttgagtt
      241 ctgagtggga aggttttggt ctcgttgttg cagaagctat ggcgtgtgaa cgtcccgttg
      301 ttgctaccga ttctggtgga gttaaagaag tcgttggacc tcataatgat gttatccctg
      361 tcagtaatca tattctgt
//
LOCUS       DAZGTR010000519          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZGTR010000519 DAZGTR010000000
VERSION     DAZGTR010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.524"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>363
                     /locus_tag="ACHLCY_005567"
     CDS             <1..>363
                     /locus_tag="ACHLCY_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0372532.1"
                     /translation="EQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRMKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYK"
BASE COUNT           83 a           84 c          107 g           89 t
ORIGIN      
        1 gagcaggaga acatcaacgg tctgggggcg tcgtctgaga tttccatgtc gggtaatttt
       61 tatctgaatc aggcccagaa cgccctgcgt gatgcctatg acaatgacgc gttgtatgcg
      121 tttaaggtgc tgtttccgtc cggtaagggc tttaagttcc tggcggaagt gcgtcagcac
      181 acctggtcat ccggtaccaa cggcgtggtg gcagcaacgt tttcactgcg tatgaaaggc
      241 aaaccggtgt cctttgtggt accgctggcg tttgtgaaaa atctggataa aacacttacc
      301 gtgaatacag gtgcgctgct gacaatgtca gtcagtgcca acgggggaac gccgccgtat
      361 aaa
//
LOCUS       DAZGTR010000520          359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZGTR010000520 DAZGTR010000000
VERSION     DAZGTR010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.525"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..241
                     /locus_tag="ACHLCY_005568"
     CDS             <1..241
                     /locus_tag="ACHLCY_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0372533.1"
                     /translation="FGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSA
                     FAADKLHVTDITKGATYPVLIDSIAVEVNNTDTAA"
BASE COUNT          100 a           79 c           96 g           84 t
ORIGIN      
        1 ctttggcgca tttgataacg aatggcatac gctggccttc cgctttgccg ggaataacag
       61 ccttcaggtg acgccggtta ttgatggtca ggatggcaca ccgttcacgc tgacgcagtc
      121 accggtcagt gcatttgcgg cggataaact gcatgtgaca gacattacca aaggtgcgac
      181 ttacccggtg ctgattgaca gcattgcggt ggaagtgaac aacacagaca ctgcggcatg
      241 ataaaaaaaa ccgccagcga caggaatgga cgctggcggt ggtaatacct atggagaaaa
      301 aataaaggaa cgatactttc gtgctctggt tttttaaatg aaaacagttc ttattgtca
//
LOCUS       DAZGTR010000521          358 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZGTR010000521 DAZGTR010000000
VERSION     DAZGTR010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.526"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>358
                     /locus_tag="ACHLCY_005569"
     CDS             <1..>358
                     /locus_tag="ACHLCY_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFP0372534.1"
                     /translation="LQKQADLLKIIMEDTSVTENTFKNIEMAITDIKREYYSHTVDIE
                     KNIHAIWVAGSPPESISDYIKTFLKTYKEFTYYLWVDEKAFGAAKFTSVLKQIAFDLA
                     CRTIQQNTPQKNIDFIN"
BASE COUNT          134 a           59 c           60 g          105 t
ORIGIN      
        1 tctacagaag caagctgatt tactaaaaat aatcatggag gatacctctg ttacagaaaa
       61 tacctttaaa aacatagaga tggctataac agatataaaa agggagtact attcgcatac
      121 agttgatatt gagaaaaata ttcatgccat atgggttgcg ggttccccac ctgaaagcat
      181 ttcggactat attaagactt ttctcaaaac ctacaaagag tttacttact atctttgggt
      241 tgatgaaaaa gcatttggag ccgcgaaatt taccagtgtc ttaaaacaaa ttgcatttga
      301 tttagcatgc agaactatac agcagaatac tccacaaaaa aatattgatt ttattaat
//
LOCUS       DAZGTR010000522          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZGTR010000522 DAZGTR010000000
VERSION     DAZGTR010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.527"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>353
                     /locus_tag="ACHLCY_005570"
     CDS             <1..>353
                     /locus_tag="ACHLCY_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP0372535.1"
                     /translation="DLTASRAAGNVVGGVLGAFSPIGSETSETLSGALLKNAAPTILR
                     PFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYR
                     SGAVDITPESLKYWV"
BASE COUNT           80 a           88 c          108 g           77 t
ORIGIN      
        1 gacctgacgg cgtcccgtgc tgccggtaat gttgttggtg gtgtgcttgg tgcattcagc
       61 ccgattggca gtgagacgtc ggaaacactg tccggggcat tgctgaaaaa tgcagcgccg
      121 accattctgc gtccgtttgc gaaccttgcc atgaatgaaa acttcatggg ggcgcagatt
      181 taccaggaga acatgccgtt tggtacacca aaacctgaca gccagctggg aagacgttca
      241 acgccagaag cgtacaaggc gtttgcatcc tggctgaatg cgttctcagg tggcagccag
      301 taccgttcag gcgcggtgga tatcacaccg gaatcgctga aatactgggt gga
//
LOCUS       DAZGTR010000523          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZGTR010000523 DAZGTR010000000
VERSION     DAZGTR010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.529"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>346
                     /locus_tag="ACHLCY_005571"
     CDS             <1..>346
                     /locus_tag="ACHLCY_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFP0372536.1"
                     /translation="VRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDV
                     IDIKFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRA
                     DESIQFSYADMES"
BASE COUNT          105 a           57 c           62 g          122 t
ORIGIN      
        1 gttcgttttg ctctcagttt ccgttacccc ggctgggcaa aaatgatgat tattaatggt
       61 gttactttta ctaaaaatga tactcaaaac tcacttatta ttttagatag aacatggcaa
      121 catcatgatg tgattgacat taaatttata gctgatatac agtttaatac tgacttatgt
      181 ggtgacactt atatttccag aggtcccgtt ttatatgcaa ttgagcttga gagcgatatt
      241 ttaatcaaaa aaactctgct gaacgggaaa tattttgact caggatacat accctgtagt
      301 agagctgatg aatctatcca gttctcttat gctgatatgg aatctt
//
LOCUS       DAZGTR010000524          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.530, whole genome shotgun sequence.
ACCESSION   DAZGTR010000524 DAZGTR010000000
VERSION     DAZGTR010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.530"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
BASE COUNT          111 a           67 c           64 g          104 t
ORIGIN      
        1 tcttccttaa atggttgcct tctgaaaatc aagattgagg gccgtgttaa cattacgcgg
       61 gtaagatcat tgaacataca tagctcagca gttagcagat aaggctgaag caacgtatca
      121 acgagggaac gcggttgcgc tgaaggatga ttggacggcg tattctttta ccaatattta
      181 actgctctgt ttgaaaacac cgttatgttc cctatctctc ataaaggaca acttcctgac
      241 accagctttt ctggttgtat taaataaatg ataagggaaa agctattacc aatatataaa
      301 tacaatgaat atacataatc tattcatcac aactatttat tctccg
//
LOCUS       DAZGTR010000525          342 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.531, whole genome shotgun sequence.
ACCESSION   DAZGTR010000525 DAZGTR010000000
VERSION     DAZGTR010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.531"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>342)
                     /locus_tag="ACHLCY_005572"
     CDS             complement(<1..>342)
                     /locus_tag="ACHLCY_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0372537.1"
                     /translation="INLSLCHCSSLKHIEGSFPEALRNSVYLNGCNSLNESQCQFLAY
                     DVSQGRACLSKAELTADLIWLSANRTGEESAEELNYSGCDLSGLSLVGLNLSSVNFSG
                     AVLDDTDLRMSD"
BASE COUNT          115 a           76 c           58 g           93 t
ORIGIN      
        1 atcactcatc ctgagatctg tatcatcaag cactgctcca gaaaaattta ctgatgataa
       61 attcagccct acaagactta gacctgacaa gtcacatcca gagtaattca attcttcagc
      121 agactcttca cccgttcggt tagctgacaa ccaaattaag tcagcagtaa gctcagcttt
      181 gctcaggcag gcacggcctt gactgacatc atatgcaagg aattgacatt gcgattcatt
      241 taatgaatta cagccattta aatatacgga atttctgagt gcctcaggaa aggaaccttc
      301 tatatgtttc aaagaactac aatggcataa agataaatta at
//
LOCUS       DAZGTR010000526          341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.532, whole genome shotgun sequence.
ACCESSION   DAZGTR010000526 DAZGTR010000000
VERSION     DAZGTR010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.532"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            101..>341
                     /locus_tag="ACHLCY_005573"
     CDS             101..>341
                     /locus_tag="ACHLCY_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HFP0372538.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTYFL"
BASE COUNT          111 a           64 c           50 g          116 t
ORIGIN      
        1 aaccctattc cctcaatgtt aagcaaatat aacaattact cttattttaa attttacacg
       61 taatatttac cctaaatgat tttagagtac aggtgggcat atgcaagtct tttccagtga
      121 tgtctacttc actgtaggca ctaatgcatt attagcatcc caaaaagaat attacagcga
      181 tttagttgcg ttggttgatc ttggacattc gttcgttgtt attgatgagc atcaacatcg
      241 aaatctcaaa ccaaatacag aacctgtaaa catccttttg agcaacaatt tcatcaggat
      301 caataagaat attacattat ctgatttaac gtatttcctg g
//
LOCUS       DAZGTR010000527          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.533, whole genome shotgun sequence.
ACCESSION   DAZGTR010000527 DAZGTR010000000
VERSION     DAZGTR010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.533"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>331)
                     /locus_tag="ACHLCY_005574"
     CDS             complement(<1..>331)
                     /locus_tag="ACHLCY_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372539.1"
                     /translation="INKTYGLKYAPVDGDGYCLLRAILVLKEHEYSWALGSHKTQKQV
                     YEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQSDNKIQGQGGFLSWSFLSFKK
                     EFIDSCLN"
BASE COUNT          102 a           74 c           45 g          110 t
ORIGIN      
        1 attcaagcaa ctatcaataa attctttttt aaaagacaaa aaactccatg ataaaaaacc
       61 accctgcccc tgaattttgt tgtcagattg tagattaacg ccgaataatg tctttacatc
      121 ttccctgaga tcattgaatg ccgtatcaac aagagcctcg atcgtttgtt tatcaacaat
      181 tttaataaac tcttcgtata cttgcttttg cgtcttatgg ctgcccagcg cccatgaata
      241 ctcatgttct tttaaaacca gtatggccct taacagacaa tagccatcgc catctactgg
      301 agcgtatttt aatccataag ttttattaat t
//
LOCUS       DAZGTR010000528          329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.534, whole genome shotgun sequence.
ACCESSION   DAZGTR010000528 DAZGTR010000000
VERSION     DAZGTR010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.534"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            <1..>329
                     /locus_tag="ACHLCY_005575"
     CDS             <1..>329
                     /locus_tag="ACHLCY_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0372540.1"
                     /translation="DALAQKIREGWQPYGGPFSSYTDDGAALIQAIVAEGDVSTPVVV
                     KPTGGEGAVISATSDPGYYFVVVLAGQSNSMAYGEGLPLPETYDRPDPRIKQLARRST
                     VTPGGVA"
BASE COUNT           72 a           79 c          107 g           71 t
ORIGIN      
        1 gatgcacttg cgcaaaaaat tcgtgaagga tggcaaccat atggtgggcc gttttcttcg
       61 tatacggatg atggcgcagc acttattcag gcgattgtcg cagaaggtga tgtgagcaca
      121 cctgttgtgg tgaagccgac aggtggagaa ggtgcagtaa tcagcgccac cagcgacccc
      181 gggtattact ttgttgtggt tctggcaggg cagtcaaaca gcatggcata tggtgaaggc
      241 cttccgctgc cggagacata tgaccgtccg gacccgcgca ttaagcagct ggcgcgtcgc
      301 agtacggtga caccgggcgg tgtcgcctg
//
LOCUS       DAZGTR010000529          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.535, whole genome shotgun sequence.
ACCESSION   DAZGTR010000529 DAZGTR010000000
VERSION     DAZGTR010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.535"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(57..>328)
                     /locus_tag="ACHLCY_005576"
     CDS             complement(57..>328)
                     /locus_tag="ACHLCY_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372541.1"
                     /translation="KYLSIFEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMD
                     GNVQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDYVSMMLP"
BASE COUNT          116 a           68 c           48 g           96 t
ORIGIN      
        1 gataaatata aaataggcat ggcgaaaaaa aaatcaccac gcctaaaata taataattat
       61 ggtagcatca ttgatacata atccacacca atccttgagc tatactgaga cgctatagcc
      121 tgatatcttt ctgcataacc agttctcagt tgagatttaa gtttgagtcg gacaggaaca
      181 ttttgcacgt tgccatccat attacttaaa aacacggcag aaataatatt tttttcttcg
      241 ccatcaactg ttgttccatg attcaacacc accatataat caacaacagg aagcgtttta
      301 tccccttcga aaatagagag atattttc
//
LOCUS       DAZGTR010000530          325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.537, whole genome shotgun sequence.
ACCESSION   DAZGTR010000530 DAZGTR010000000
VERSION     DAZGTR010000530.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.537"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(<1..>325)
                     /locus_tag="ACHLCY_005577"
     CDS             complement(<1..>325)
                     /locus_tag="ACHLCY_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372542.1"
                     /translation="ANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTSTY
                     ASGTITVRISDDQKFDRQVMIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEI
                     FNQSTT"
BASE COUNT           72 a           78 c           76 g           99 t
ORIGIN      
        1 gtcgtggact gattaaaaat ctcctgacca ttgcgggtca ctcttaaccg gcaggttgaa
       61 taccagtatg actgttggtt attactgttg aaattctcat gcttaccacc gcggaataac
      121 actggcggta tcatgacctg ccggtcaaac ttctgatcat cactgattct taccgtgatg
      181 gtgccactgg cataagtgct cgtgcggggg aaagacttgc tgaccgtttt gacaatatcg
      241 ccttcaatct ggttggctga cagtttcccc ttaatctgac agttctcatt aatcgtgacg
      301 ttgttgagcg tccctgagtt cgcat
//
LOCUS       DAZGTR010000531          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.denovo.538, whole genome shotgun sequence.
ACCESSION   DAZGTR010000531 DAZGTR010000000
VERSION     DAZGTR010000531.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.denovo.538"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            complement(158..>323)
                     /locus_tag="ACHLCY_005578"
     CDS             complement(158..>323)
                     /locus_tag="ACHLCY_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFP0372543.1"
                     /translation="ISRAGGPLESVIYHEQQIRDEQERQARLERERARSWHEEQEREA
                     RVWRGPSMGM"
BASE COUNT           74 a          110 c           75 g           64 t
ORIGIN      
        1 gatgacatac gcagacatcg cgggatgagg ctgaggaatg agcgcgatct ggcaaagagg
       61 caaaacacag caacaaaaac gacacgccag aatcgcgccc ggatgcgttt ttaacgcgtt
      121 ccggtaccat ctggcaacct cccggaacaa ctcaccgtca catccccatt gacgggccac
      181 gccatacccg tgcttcccgt tcctgctctt catgccagga ccgcgcacgc tcccgttcca
      241 ggcgtgcctg cctttcctgt tcatccctta tctgctgttc gtgataaata accgactcaa
      301 gcggtccacc tgcccggcta atc
//
LOCUS       DAZGTR010000532        15981 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.guided.098, whole genome shotgun sequence.
ACCESSION   DAZGTR010000532 DAZGTR010000000
VERSION     DAZGTR010000532.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.guided.098"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            114..1082
                     /gene="asd"
                     /locus_tag="ACHLCY_005579"
     CDS             114..1082
                     /gene="asd"
                     /locus_tag="ACHLCY_005579"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0372544.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            1104..4427
                     /gene="mscM"
                     /locus_tag="ACHLCY_005580"
     CDS             1104..4427
                     /gene="mscM"
                     /locus_tag="ACHLCY_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0372545.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4456..4770)
                     /locus_tag="ACHLCY_005581"
     CDS             complement(4456..4770)
                     /locus_tag="ACHLCY_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFP0372546.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(4767..5081)
                     /gene="yjeN"
                     /locus_tag="ACHLCY_005582"
     CDS             complement(4767..5081)
                     /gene="yjeN"
                     /locus_tag="ACHLCY_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFP0372547.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(5133..6635)
                     /gene="yjeM"
                     /locus_tag="ACHLCY_005583"
     CDS             complement(5133..6635)
                     /gene="yjeM"
                     /locus_tag="ACHLCY_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0372548.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(6854..7831)
                     /gene="epmA"
                     /locus_tag="ACHLCY_005584"
     CDS             complement(6854..7831)
                     /gene="epmA"
                     /locus_tag="ACHLCY_005584"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0372549.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            8156..9964
                     /gene="frdA"
                     /locus_tag="ACHLCY_005585"
     CDS             8156..9964
                     /gene="frdA"
                     /locus_tag="ACHLCY_005585"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0372550.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            9957..10691
                     /gene="frdB"
                     /locus_tag="ACHLCY_005586"
     CDS             9957..10691
                     /gene="frdB"
                     /locus_tag="ACHLCY_005586"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0372551.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            10702..11097
                     /gene="frdC"
                     /locus_tag="ACHLCY_005587"
     CDS             10702..11097
                     /gene="frdC"
                     /locus_tag="ACHLCY_005587"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0372552.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            11108..11467
                     /gene="frdD"
                     /locus_tag="ACHLCY_005588"
     CDS             11108..11467
                     /gene="frdD"
                     /locus_tag="ACHLCY_005588"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0372553.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            11530..12663
                     /locus_tag="ACHLCY_005589"
     CDS             11530..12663
                     /locus_tag="ACHLCY_005589"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0372554.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            12752..13285
                     /gene="blc"
                     /locus_tag="ACHLCY_005590"
     CDS             12752..13285
                     /gene="blc"
                     /locus_tag="ACHLCY_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0372555.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(13282..13599)
                     /gene="sugE"
                     /locus_tag="ACHLCY_005591"
     CDS             complement(13282..13599)
                     /gene="sugE"
                     /locus_tag="ACHLCY_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0372556.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(13781..13927)
                     /gene="ecnB"
                     /locus_tag="ACHLCY_005592"
     CDS             complement(13781..13927)
                     /gene="ecnB"
                     /locus_tag="ACHLCY_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0372557.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(14038..14163)
                     /gene="ecnA"
                     /locus_tag="ACHLCY_005593"
     CDS             complement(14038..14163)
                     /gene="ecnA"
                     /locus_tag="ACHLCY_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0372558.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(14215..14781)
                     /gene="efp"
                     /locus_tag="ACHLCY_005594"
     CDS             complement(14215..14781)
                     /gene="efp"
                     /locus_tag="ACHLCY_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0372559.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            14823..15851
                     /gene="epmB"
                     /locus_tag="ACHLCY_005595"
     CDS             14823..15851
                     /gene="epmB"
                     /locus_tag="ACHLCY_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0372560.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4002 a         4224 c         4138 g         3617 t
ORIGIN      
        1 ctgatacgga tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg
       61 atccggcatg ttggccggat caggaacgac aaaacaatgg cctggaggct accttgttaa
      121 attcatttaa actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg
      181 gttggggcgc aagcaagcgg gcaggatggc tgacaaaact ggttatcgat ctgttcgtta
      241 aatactacaa ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct
      301 ttaacgaatt ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga
      361 atgtcctggt catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata
      421 aaatcctgca agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc
      481 tgatggcgga cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact
      541 accatcgcgt acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg
      601 atctcttctc cgttaaccat ctcaccgctc agaacgtgcc gaatctgttt gcccgtaacg
      661 aacgcgtgat ttgcctgttc gataccgaat ttggcccgat ggcgcagatt ctggtcgggg
      721 cgacgattgt aggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag
      781 gtatcatcaa gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga
      841 aaggccagga aatgggccgc tttaaactcg gctctaccgt tatcaacctg tttgcaccgg
      901 gtaaagtgaa tctggttgag caactggaaa gcctgtctgt cacgaaaatt ggccagccgc
      961 tggcagtatc taccgaaacc tttgttacgc cagacgctga acccgcccca cttcctgctg
     1021 aagagatcga agcagaacac gacgccagcc cattggttga cgataaaaaa gaccaggtct
     1081 aatccatcaa aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc
     1141 tcagttgggg ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg
     1201 agcaggcaaa agcggcgaaa cccgcacagc cggaagtcgt agaggcgctc cagtctgcct
     1261 taaatgcgct tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta
     1321 tcgataatta tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgacg
     1381 agccgcgcag cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg
     1441 tcagcagcca gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg
     1501 agattgccga ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa
     1561 atgagatcga gcgccgcctg ggaacgctta ctggcaatac cccgctcaat caggcacaaa
     1621 atttcgcgtt gcagtctgac tccgcacgcc tgaaagcgct cgttgatgaa ctggaactgg
     1681 cgcagctgtc tgccaataac cgtcaggaat tagcgcgctt acgctcagag ctggcggaaa
     1741 aagagagcca gcaactggat gcgtatttgc aggccttacg taatcagttg aacagccaac
     1801 gtcagcttga ggcggagcgg gcgctggaaa gtaccgaatt gctggcagaa aacagcgccg
     1861 atttgccgaa agacatcgtc gcgcaattca aaattaaccg cgaactatcg gcggcactga
     1921 atcaacaggc gcagcggatg gatctcgttg cctcgcaaca gcgccaggcc accagccaga
     1981 cgttacaggt tcggcaggcg ctgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt
     2041 ccaacctgct cggcgaagcg ttgcgggcgc aggtggcacg gctgccggaa atgccgaaac
     2101 cacaacagct tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc
     2161 tgcttaataa acagccgctg ctacggcaaa ttcatcaggc cgacggtcag ccgctgaccg
     2221 ccgagcaaaa ccgtattctg gaagcacagc tacgcactca gcgtgagttg ctgaactcat
     2281 tgttgcaggg tggcgacacg ctactgctgg aactgaccaa gctgaaagtc tccaacgggc
     2341 aacttgagga tgcgctgaaa gaggtgaatg aagcgacgca tcgctatctg ttctggacct
     2401 ctgacgtgcg cccgatgacc atcgcctggc cactggaaat cgcccaggat ctgcgtcgtc
     2461 tcatttcgct ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca
     2521 aagagacgat tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc
     2581 gccgctattt cacccgtttt cttgaacgtt cggcggcgaa agtcggcaaa gtgactcagg
     2641 atcacttctg gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgctgc
     2701 cggtgctgtg gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg
     2761 tcgcgattgg cgatggcgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg
     2821 ccacctttgc ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgtg
     2881 tttcccgtgg gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg
     2941 cgctgatgat gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggacggcttt
     3001 gctttatcct catctgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga
     3061 tcccgctgta cctcaacaaa gagggcagcg gcgacaacat taccaaccat atgctgtgga
     3121 acatgatgat tggcgcacca ctggttgcca ttctggcgtc agcggtgggt tatctggcaa
     3181 cggcgcaggc actgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg
     3241 tggtttatca cgttatccgc cgctggatgc tgatccagcg gcgcaggctg gcgtttgacc
     3301 gggcgaagca tcgccgggca gagatgttag cgcaacgcgc gcgtggcgaa gaggaagcgc
     3361 atcatcacag tagcccggaa ggggcaattg aagtcgatga aagcgaagtc gatctcgatg
     3421 ccatcagtgc gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt
     3481 ctgtcattgt gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc
     3541 tgtgggatgt cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg
     3601 cggtgctgat tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg
     3661 cgctgctgga actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca
     3721 tcaccaccat caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga
     3781 ttggtattga gtggtcgaaa ttgcagtggc tggttgccgc gctcggtgtt ggtctcggtt
     3841 ttggtttgca ggagattttc gccaacttta tctctggcct gattatcctg ttcgaaaaac
     3901 cgattcgcat tggcgatacg gtaacgatcc gcgatcttac cggtagcgtg acgaaaatta
     3961 acacccgcgc caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg
     4021 cgtttattac cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtggtgt
     4081 tgacgatacc ggcccctgcc gatgccaata gcgaagaagt gacggaaatc ctgctcaccg
     4141 cagcgcgtcg ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc
     4201 tgcaacaggg gattcagatt ttcgagctgc gtatttacgc cgctgagatg ggtcaccgta
     4261 tgccgctacg ccatgagatc caccagctga ttctggcagg cttccacgcc cacggtatcg
     4321 atatgccatt cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga
     4381 gaacgctgac gtctgcgggc aaaggtcgtc aggcgggaag tttgtaagtt tgtgtgccgg
     4441 atgaaccaat ccggctcaca aacaaaactg atagcgcaga aaaaactgcc acagccagta
     4501 gccgaaaata gccagcgcgg tggcgtacat aaaccagccg cgtttttttc gccacaaagc
     4561 gaaccagaga aaagggaaaa agagcggtaa cgtcattatc acaccaacat cacgggtatc
     4621 gtcttcttcg agatacatca acacatcgca gacgttgtta atcccgcgtc catcaatcat
     4681 ccactctttg tccagcgcgg aagtgatggt tatccagagg aacgccacaa taaaccagat
     4741 gcagcataag acaaacatgc gcgcactcat aaggtaaccg gtcgcttaat ttcgctatag
     4801 cgcatacgaa acaaatcgcc gtatcccact aattccccgc gaaaaaccag acatcggata
     4861 agataagcgc aataaatatc cggcaggttt ccccaccgtt taccatcgcg atctttgagg
     4921 gtgttaatgg tgctacccac caccatcgcc actttacggg tgtggcaggc atcgacaaaa
     4981 gagagaatcg tctgctctat ttccgctaca tccccggcag aaaattgcag cgccctgatt
     5041 tcatcttcgg ttattgccgg gtcccggctg gtgtcatcca taacatttcc tgtctttacg
     5101 ccctatcgtc aggtagacga tagggcatcg ggttattcca ccgcccactg cggatttttt
     5161 gccactctgc tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa gaagacaggg
     5221 ccgccaatca tccacaatgt gctgtcccag tctccggctt caaccacagg ttgaatgatg
     5281 gtgaagacgt tcgcaaatgt caccaccagt acgacaacca ctgttgcaat cattgccgac
     5341 atatgcgttt tgaaaatcac aaatggtctg tcgagatcct gacgtgcttt aaaaaatggg
     5401 aaagccagcg cgaggaacag gtaaggaagc gtcatagaca cgttcgccat cagcgtcagc
     5461 ttgttaaaga acgccgatgc ggtaccgcca ccaaacgaaa ccagcaggat gaagacagta
     5521 accaacccgc actgcatcca catggcgata gacggcatcc ccatcgcatt caggcgcgtc
     5581 atcggttccg gccacaacgc tttcggcgtc ccctggatga tggctttcag cggtgaatag
     5641 caaagcgtaa agaacgcacc ggtataagcg aggaacatcg aaagtccggt aatacgcgca
     5701 aaccatacgc ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt acccagcgtc
     5761 accccgaggc tcttcatcag cacataggta atattgccaa ggttaacgga accattactt
     5821 aatacctgct gccagtttgt gctgacgccc cataaaaata ttgccagcga ataaccgatt
     5881 gaaataacaa tagcggcaaa aacaatacct ttggcaaagt tcttttctgg attttccgtt
     5941 ttatcgacca gaccaccaac cgcttcaatt ccgccatagg caaaaatagc aaataccaca
     6001 aacgatagca ttgccagacc ggactgataa cctgggttcg gtgatgcaag gaaattaata
     6061 tcctgcgcga aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact
     6121 aaattcagac acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc
     6181 ccctttgaag caacgacggt gaccagaatc atccatgcca ctgccagcag accaaccacc
     6241 tgcgtaggct ccagtccagc aatacgccag tgctgggtca tgtcgctacc atagaggaat
     6301 gttgagaacg gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa
     6361 aaccacataa acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa
     6421 tagataccgc cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat
     6481 ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag
     6541 taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt
     6601 cctatcagac tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt
     6661 gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa
     6721 tacagaattg atacctggat gatatcaaat atccagtctt aaattgtggc ctttaacgga
     6781 aaaatgaaaa catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag
     6841 taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg
     6901 cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca
     6961 taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg
     7021 ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgtcaatt
     7081 catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc
     7141 gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa
     7201 aggtcggttt ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagca
     7261 gcgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt
     7321 cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt
     7381 gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca
     7441 cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact
     7501 cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg
     7561 gcccacaacc ggcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga
     7621 gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa
     7681 tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg
     7741 caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg
     7801 cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca
     7861 cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg
     7921 gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga
     7981 taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc
     8041 agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc
     8101 aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca
     8161 aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat
     8221 tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat
     8281 gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag
     8341 cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt
     8401 cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg
     8461 cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat
     8521 cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca
     8581 gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct
     8641 ggttgatgat ggtcatgttc gcggcgtggt agcaatgaac atgatggaag gcacgctggt
     8701 gcagatccgt gctaacgcgg tcgttatggc tactggcggt gcgggtcgcg tttatcgtta
     8761 caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt
     8821 tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat
     8881 cctgatgacc gaaggctgcc gcggtgaagg cggtattctg gtcaacaaaa atggctaccg
     8941 ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata
     9001 tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg
     9061 caacaccatc tccacgccgc gtggcgatgt ggtttatctc gacctgcgtc acctcggcga
     9121 gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt
     9181 cgatccggtt aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat
     9241 cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc
     9301 ctctgttggt ctgcacggtg caaaccgtct gggttctaac tccctggcgg aactggtggt
     9361 cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa
     9421 cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa
     9481 ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga
     9541 aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga
     9601 gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga
     9661 cctgctctac accattgaac tgggccacgg tctgaacgtt gctgaatgta tggcgcactc
     9721 cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga
     9781 gcgtgacgac gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac
     9841 tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg
     9901 tggcgaagcg gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg
     9961 ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac
    10021 cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg
    10081 gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga
    10141 tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc
    10201 tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg
    10261 atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca
    10321 tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa
    10381 agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt
    10441 ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg
    10501 aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg
    10561 tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg
    10621 ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga
    10681 aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg
    10741 tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg
    10801 gttccggctg tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc
    10861 ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac
    10921 ctgatcactc tggcggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa
    10981 gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc
    11041 tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga
    11101 gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt
    11161 cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat
    11221 tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt
    11281 cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg
    11341 tggtttacac cgtatgcacc acgcgatgca cgatctgaaa attcacgtgc ctgcgggcaa
    11401 atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg gtatcgttac
    11461 aatctaacgt atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa
    11521 ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttgctccaca
    11581 tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag
    11641 caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt
    11701 acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag
    11761 ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg
    11821 gaaatcaagt taagcgatcc cacaacaaaa tactggcctg aacttaccgc taaacagtgg
    11881 aatgggatca cactattaca tctcgcaacc tacactgctg gcggcctgcc attgcaggtg
    11941 ccggatgagg tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca
    12001 tgggcgccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg
    12061 gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaacacgtgt cttccagcca
    12121 ctcaaactca accatacgtg gattaatgta ccgcccgcag aagaaaagaa ttacgcctgg
    12181 ggatatcgcg aaggcaaggc agttcatgtt tcgccagggg cgttagatgc tgaaacttat
    12241 ggtgtgaagt cgaccattga agatatggcc tgctgggtac gaagcaatat gaatccccgt
    12301 gatatcaacg acaaaacact tcagcaaggg atacaactgg cacaatctcg ctactggcaa
    12361 accggcgata tgtatcaggg cctgggctgg gaaatgctgg actggccggt aaatcctgac
    12421 agcatcatta acggcagtgg caataaaatt gcactggcag cacaccccgt aaaagcgatt
    12481 acgcccccaa ctcctgcagt acgcgcatca tgggtacata aaacaggggc aaccggcgga
    12541 tttggtagct atgtcgcgtt tattccagaa aaagagctgg gtatagtgat gctggctaac
    12601 aaaaactatc ccaatccagc gagagtcgcc gccgcctggc agattctcaa cgctctacag
    12661 taaaatttca tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta
    12721 cacttagaaa aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc
    12781 gacagctgca tttctggtcg ttgcctgcag ttctcctacg ccgccgcgtg gcgtaacagt
    12841 agtgaataat ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga
    12901 tcaccgtttt gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg
    12961 cggcctgaat gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga
    13021 agggaaagcg tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg
    13081 tcctttctat ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt
    13141 ttgcggcccg gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga
    13201 agtgaaacag gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat
    13261 ttgggtacag cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac
    13321 gattaacgcc agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc
    13381 ggtgatggcc gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg
    13441 taacgatttc atcgcccagg caagtagcgc catactgaca atcatcgccg tcacggtaat
    13501 aacactcggc gtcaaacgac taaagccgtg ggtatatttc aggccaacgg cccataccac
    13561 ttccagcaga ccagcaataa ctaagataat ccaggacata tcaggctccg gagaattggg
    13621 gccgtccccg gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga
    13681 tattttgcca ggtggcaggg gaatttcaac tattacctta gtgactatcg cagaaaaaac
    13741 aaacggcacg acatacctgt cgtaccgtta ttgcttattg ctattgctgc gctttcgttg
    13801 ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca cgcgtggtgt
    13861 tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca attgtcttct
    13921 tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc aaattaataa
    13981 tagacaacat cgccaaaaat gacggaaatc tgatgatttt tcggaagaga agaaaaatta
    14041 gctggcagcg cgagagatgg agttgccgag atgtttgatg tcttcgccga aaccgcgagc
    14101 ggtgttacag cccgtgagca gcgtgctggc aagcaaaaca agaacgataa ggcgtttcat
    14161 catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac cgcattactt
    14221 cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc cgatttgtac
    14281 gaacagcgga accttaacca cagcgccagt agacagggta gccggtttgc cgccagtacc
    14341 tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa cgaagttcgg
    14401 cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg cctgatccag
    14461 cagccattta gcgttgtcgc caattgcttt tgcatcagca gacagctgct cgaaagtttc
    14521 gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt tcatatcgac
    14581 aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc gagtaccggt
    14641 cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt ttacgaattc
    14701 actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag cacgaaaatc
    14761 gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag ctaagccaca
    14821 aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta acgcaacttg
    14881 ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac gcggacgaaa
    14941 aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc cgctcattta
    15001 tcgatcgcat ggagaaaggc aatccggacg atcctctttt gcgtcaggta cttacctcgc
    15061 aagatgagtt tgtcgtcgcg tccggattct ccaccgaccc gctggaagaa cagcacagcg
    15121 tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc aaaggcggct
    15181 gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa aatcagggca
    15241 acaagcgtaa ctggcagact gctcttgagt atgttgctgc gcatccggaa ctggacgaga
    15301 tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac tggttgctca
    15361 cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt ctgccgattg
    15421 tgatcccggc acgtatcacc gaggcgctgg ttgaacgctt tgcccgttct acgctgcaaa
    15481 tcttgctggt gaatcacatc aatcatgcca atgaggtaga tgaaacgttc cgtcaggcga
    15541 tggctaaatt gcgccgtgtc ggtgtcaccc tgcttaacca gagcgttctg ttacgtggtg
    15601 tgaacgataa cgcacaaacg ctggcaaacc tgagtaatgc gttgttcgat gccggcgtaa
    15661 tgccgtatta cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt atggtgagtg
    15721 atgacgaagc acggcagatt atgcgtgagt tgctgacact ggtgtcgggt tatctggtgc
    15781 cgaaactggc gcgagaaatc ggcggcgaac ccagcaaaac gccgctggat ctccagctac
    15841 gccagcagta actgattaaa cagggaagtt aaacattagc ctgatgcgct acgcttatca
    15901 ggcctacatt gtgcattgaa attgaatgga tttgcaatga actgtaggcc ggataaggcg
    15961 tttacgccgc atccggcaag a
//
LOCUS       DAZGTR010000533        15035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.guided.106, whole genome shotgun sequence.
ACCESSION   DAZGTR010000533 DAZGTR010000000
VERSION     DAZGTR010000533.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.guided.106"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            125..1441
                     /gene="fliD"
                     /locus_tag="ACHLCY_005596"
     CDS             125..1441
                     /gene="fliD"
                     /locus_tag="ACHLCY_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0372561.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQTELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSTSAPVATDVSSAQDAIIHLGS
                     ATGPEITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSTSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFNDEMAKNPDGLTSIFVGDNSMVAQMDDLINTYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            1464..1856
                     /gene="fliS"
                     /locus_tag="ACHLCY_005597"
     CDS             1464..1856
                     /gene="fliS"
                     /locus_tag="ACHLCY_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0372562.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            1861..2172
                     /locus_tag="ACHLCY_005598"
     CDS             1861..2172
                     /locus_tag="ACHLCY_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HFP0372563.1"
                     /translation="MEKQRRKLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLDAVKARLYDAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            2169..3230
                     /locus_tag="ACHLCY_005599"
     CDS             2169..3230
                     /locus_tag="ACHLCY_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0372564.1"
                     /translation="MNPTLLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRQTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAITPTQ
                     PRVSTQPARPKSELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTQELLSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSFGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            3238..3705
                     /locus_tag="ACHLCY_005600"
     CDS             3238..3705
                     /locus_tag="ACHLCY_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HFP0372565.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            3725..4441
                     /locus_tag="ACHLCY_005601"
     CDS             3725..4441
                     /locus_tag="ACHLCY_005601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HFP0372566.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLVGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            4454..5317
                     /gene="motA"
                     /locus_tag="ACHLCY_005602"
     CDS             4454..5317
                     /gene="motA"
                     /locus_tag="ACHLCY_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0372567.1"
                     /translation="MQKILGLLVVFGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            5320..6243
                     /gene="lafU"
                     /locus_tag="ACHLCY_005603"
     CDS             5320..6243
                     /gene="lafU"
                     /locus_tag="ACHLCY_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP0372568.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            6314..7369
                     /gene="dinB"
                     /locus_tag="ACHLCY_005604"
     CDS             6314..7369
                     /gene="dinB"
                     /locus_tag="ACHLCY_005604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP0372569.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            7366..7818
                     /locus_tag="ACHLCY_005605"
     CDS             7366..7818
                     /locus_tag="ACHLCY_005605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0372570.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            8064..9203
                     /locus_tag="ACHLCY_005606"
     CDS             8064..9203
                     /locus_tag="ACHLCY_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP0372571.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            9200..9814
                     /gene="prfH"
                     /locus_tag="ACHLCY_005607"
     CDS             9200..9814
                     /gene="prfH"
                     /locus_tag="ACHLCY_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0372572.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSLRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9871..11328)
                     /gene="pepD"
                     /locus_tag="ACHLCY_005608"
     CDS             complement(9871..11328)
                     /gene="pepD"
                     /locus_tag="ACHLCY_005608"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0372573.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            11589..12047
                     /gene="gpt"
                     /locus_tag="ACHLCY_005609"
     CDS             11589..12047
                     /gene="gpt"
                     /locus_tag="ACHLCY_005609"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0372574.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            12139..13383
                     /gene="frsA"
                     /locus_tag="ACHLCY_005610"
     CDS             12139..13383
                     /gene="frsA"
                     /locus_tag="ACHLCY_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0372575.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            13441..13842
                     /gene="crl"
                     /locus_tag="ACHLCY_005611"
     CDS             13441..13842
                     /gene="crl"
                     /locus_tag="ACHLCY_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0372576.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(13881..14936)
                     /gene="phoE"
                     /locus_tag="ACHLCY_005612"
     CDS             complement(13881..14936)
                     /gene="phoE"
                     /locus_tag="ACHLCY_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0372577.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         3763 a         3730 c         4026 g         3516 t
ORIGIN      
        1 gaaacgccat ttccgccaga ccggaaacgc catttccgcc tgttttttaa tctattgatt
       61 attcacataa aaaatattgg cacgcctttt gccaataacc tgacatcgtt atccggagtc
      121 atcaatgatc aacccaagaa ccattgcgca agaaatcgcg tatgcggatg tcgccactca
      181 ggcggcaaac ttgcaggaga agcaaacaga gctggatgct gaaagcagcg gcctggattc
      241 gctcagttca gcgttgagcg attttcagag cgccgttgac gcgctgaaca gcgataccga
      301 cggcccggtg acgtttgccg ccaccagcaa taatgactcg gcgaccgttt ccgccaattc
      361 ccaggcgcag gcgggaagct actcattttt cgttgagcaa ctggcacagg ggcagcaaac
      421 cacgttcagt atgggcgacg acgccttttc cgccaccggc acttttgaac tgacgatggg
      481 cgacagcacc atggatatcg atctgtcggc ggcagatcag aatggtgacg gcgacggttt
      541 tatcgacgcc agtgaactgg tgaatgcgat taacgactcc gatgataatc cgggcgtgtc
      601 ggcggcgctg gtaaaaaccg acggcaccac aacgattatg ctcacctcgg atagcaccgg
      661 ggcgcagagc gcgttttcgg tgagcgtaac ggggcatgat gccagcaacg atagcaccag
      721 cgcgcccgtt gccacggatg tttcctccgc tcaggacgcg attattcatc ttggcagcgc
      781 cacggggcct gagatcacca acagcagcaa tacgtttgat gatgtgatcc ccggtgtcac
      841 catgactttc accgaagtca gcgattctga cagcgatctc actaccttta acatcagcga
      901 agattccagc gccagccagg agaaagtaca gacctttgtc gatgcctata acaccttgat
      961 tgatacggtt gattcgctaa ccacccacgg tgatgacagt accagcgccg gggtatttgc
     1021 aggcgacgcc ggactcagtt cactggcgaa ccagctcgat gacatcgccc atgccagcta
     1081 caacggcgtg tcgattgttg actatggcat caccctcgat tctcacggcc acttacagat
     1141 tgactccgac cagttcaacg acgaaatggc gaaaaatcct gacggtctga cctctatttt
     1201 cgttggcgac aacagcatgg tggcgcagat ggacgacctt attaacacct acacggactc
     1261 cagcaacggc atcatcaccc tgcgccagca gaacattgac gatcagatga gcaaaattca
     1321 ggacgaaggc gatcagctta ctgataccta taacgccaac tacgaccgtt atctcgagga
     1381 gtacaccaac acgctggtcg aggtgtacac catgaaagcc agcatggcgg cattcgcgta
     1441 actcggaagg aatatcccta aagatgtacc aagtacagga cggctattcg caatataaag
     1501 agatcgatct cgcggcgcgt actgcggcgg catcgcctct ggaactggtg ctggtgctgt
     1561 tttctggcct gatggacgaa ctggaacgcg ccaaaagcca tattgaaggg cggcgtttcg
     1621 agaagaaagc ccagagcatc aataaatgca tcgacattct caatgcgctc accagttcgc
     1681 tggagtttga aactggcggc gagctggtgg tcaacctttc gcgactctat gaccactgcg
     1741 tttaccgatt gtatgaggcc agcggcgaac tgtcggcaga gaaaatcgat gaagtgatgc
     1801 tgatcttaag caacctgcgc gaaggctggg aagggctttc gggcaagctg ggttaagacg
     1861 atggaaaaac aacgcaggaa acttttcgca ctggtcgagg cgatgaatga agccctcgac
     1921 aagcaacgct ggcgacgcct tccggcactg catcagcagt tgatacgcca gtttcacgat
     1981 tacgcggcgg cggaaaccga tgcggcgcag ttggatgcag tgaaagcgcg actgtatgac
     2041 gcgtttaacg ccctgattaa acggcgcgag cagcgcgcag aagtgttgaa agcgcgtatg
     2101 gaacagcatc agcggcatca ggaaggcgta ctggcctact caattgttaa tttactttcg
     2161 gagaagtcat gaatccgaca ttgcttgcca cgctcggaac gcttgcggaa accgcttcgt
     2221 tgaaggcgga tattttaccg ccggtgagcg gtgaaaacgc gcccgcgttt accctgccga
     2281 aaatggcggt cgcggcggtg gcggagcgcg ttcatagcgc aaaaaccagt cagcaacagg
     2341 cgacgcgccc gcaggagaac gatccggtgg cgatgcaggc gctaatggcg ctgttacttc
     2401 cgcaacctgc cgcgccgcat caggacacgc cgcagccgcg aaacgtcgca acatcgccgg
     2461 ttatccagca attgacgaaa gcggtggtgc aaaacgcgcc gcaacgccag acgcaacagc
     2521 aggaactcac gccgttgccg ccgcagttgc aggaactgat cagtcagttg ccgcaggcga
     2581 agcccgaaca gcaggccaga ctggcgactt acgctagtga agatttacat gccattacgc
     2641 cgacgcagcc gcgcgtctca acacagccag ctcgcccgaa atctgaacta acccgtgtga
     2701 ccgcgcgccc gcaggtcgag cgtaaaacgg aaaaagtgcc ggacagtgaa ccggttattg
     2761 cgcgtgcggt gttgcaggtt aagacgcagg agctgctcag cgatcatcag gagattgtcg
     2821 ccaaaccagc cacgctttcg atggacgaac tgggcgaaaa actgacgacg cttttgaaag
     2881 atcagatcca ctttcagctc aacaaacaac agcagatctc caccatccgt ctcgatccac
     2941 cgtcgtttgg caagctcgag atcgccgtac aactcgacaa cggcaaactg atggtgcaca
     3001 tcggcgcgaa ccaaagtgaa gtttgccgcg cgttacagca gtttagcgac gatctccgcc
     3061 agcatctgac ggcgcaaaat tttatggagg tgaacgtaca ggtttcctcc gaagggcagt
     3121 cgcagcaaca acaacagtcg ggccatcagc aggaagaggt gagtgccgcc ttacagcttg
     3181 atgatgcgcc tcaatttcaa cagaacgaat ccgttttgat caaagtgtaa aggcaatatg
     3241 aagaaaatcg tggtggcggg cgtggtcagc gccgttctgg cgttggtggt gggggccgga
     3301 gctggatggg gtgtctggca tcattatgcc ggaaaaggaa aaccgactgc cgccgcgcag
     3361 acggaaacgg tggaaaccct ggatgaaagc aaaagcgtgt ttgtgacgtt gccggaaacc
     3421 attgtcacgc tgcatgacaa caacggcgcg gaccattatt tgtcggcgga attagtgatg
     3481 gtggtcgcca gtgacaaaga agcggaaaaa ataaaacatc aggagccgct gtatcaaagt
     3541 attgcggttg aatgcctgac ggaaatgaaa tttgaagatt tgcgcggcat gaaaatatct
     3601 gccatccgca aactcatttc tgacgcgtta aagaaagatc ttcagcgtcg caaaatgagc
     3661 gcaccgtata aggatttgct ggtgaaaaaa gtggtcttcc agtaatcacc aggcggatat
     3721 aaacctgcta caggatgtat tcgagtcaga agaatttgct gccgcgccgg tattaacccc
     3781 gcagcaggag agccactact tgcaggccta tttaccgttg gtgcgtaaag tggtgcggca
     3841 acttgccccg cagtgtaatt gcattattga tcgtcaggat atggaacaaa cggcgttaat
     3901 ggggctgctt aatgccatcc gccgctacgg gctgccggac gaaggttttg cgggctatgc
     3961 cgtgcaccgt attcgcggcg cgatcctgga tgagctgcgc gctctcgact ggcgaccgcg
     4021 ccagctgcga cagaaatatc atcagatgaa agatctgatt cgcgaaacgc gcaaaaaact
     4081 ggggcacgaa ccggagtggt cagaactggc cgtggaaggg atctcacatg aagattatct
     4141 cgaatatcag caactggaag gggcggaaac gctcgccagc ctggacgaac tgctgaacgg
     4201 cgaagggcca ggcattccgc ttgttggccg ggagctggaa gatcagtttg tcactcagca
     4261 aatgctgcaa caggcgcttt cacaattaag cgaaaaagag caactgatcc tctccatgta
     4321 ttaccagcat gagatgaatc tcaaagagat tgcgctggta ttagggctaa ccgaggcccg
     4381 aatttgtcaa ctgaataaac agatcgcgca gaaaattcgt gattttgtct atccgaatta
     4441 aagtgaagaa atcatgcaaa aaatattagg tttactggtg gtgttcggct gcgtatttgg
     4501 cggttatttt gaagcgggcg ggcaattagt ggctatctgg caaccggcgg agatgatcat
     4561 tattttgggt gccggtttcg gggcgatgat tattggtaac ccgaaacatg tgctgaaaga
     4621 gattgcccat caaattaaag gtgtgatcag taaaaaacag ctcgggccgg agtttcagcg
     4681 tcagctatta atgtgcctgt atgagttgct cgaaatggtg caaaacggtg gtctgcgcat
     4741 gctggatcaa catattgagc aaccggaaga gagcaccatt ttccagaaat acccgctggt
     4801 gttaacccag aagcggctgg tgacctttat tgccgataac tttcgcctga tggcgatggg
     4861 taaaatcgac gcccatgaac tggaaggcat tctcgatcag gaactggaca ctgcggaaga
     4921 gtcgctgctc acgccgtcgc gctcgctgca acgtaccgcc gaagcgatgc cgggatttgg
     4981 tatttgtgca gcagtgctgg ggatcatcat caccatgcag tcgattgacg gctctattgc
     5041 cctgatcggc ctgaaagtgg cggcagcgct ggtggggacg tttttaggcg tctttatctg
     5101 ctactgcctg atggacccgc tcgccaacgc catggaacaa caggcgcggg cagaacattc
     5161 tttgctggaa tgcgtgcgta ctgtgctggt ggcgcaggca ggcggcaaac cgacgctgct
     5221 ggcggtggat gccgggcgta agctgctgca tctggcgtcc aaaccgacct tcgccaacct
     5281 cgacgcgtgg gtgaatgcga tgctggagca agaataagca tgagaaagac gggcaaccgc
     5341 cgcgagcgtg gggcaaaaac caccatcgtc cggcggcaga tcaaaaagaa tcatgccggg
     5401 catcatggcg gggcgtggaa ggtggcgttc gccgacttta ccctggcgat gatggcgctg
     5461 tttatgacgc tgtggattgt caacagcgtc agtaaatccg agcgtgaaag cattatcgcc
     5521 gcgctgcacg ggcagtcgat ttttaacggt ggcgggctgt cgccgttaaa taaaatcagc
     5581 ccgtctcatc cgccaaaacc tgccaccgtg gcggtgccgg aagagacgga gaaaaaagcg
     5641 cgtgatgtga atgagaaaac ggcgctgctg aagaagaaat ccgccaccga gcttggcgag
     5701 ctggcaacca gtatcaacac cattgcccgt gatgcgcata tggaagcgaa tctggagatg
     5761 gagattgttc cccagggatt acgcgtactg attaaagacg accagaatcg caatatgttc
     5821 gaacgcggca gcgcgcagat tatgccgttc tttaaaacgc tgctggtgga gctcgcgcca
     5881 gttttcgact cgctcgataa caaaattatt attaccgggc ataccgatgc gatggcttac
     5941 aaaaacaata tctacaacaa ctggaacctt tcgggtgacc gcgcgctttc ggctcgtcgg
     6001 gtgctggaag aggccggaat gccggaagat aaagtgatgc aggtaagcgc aatggcggac
     6061 cagatgctgc tggattccaa aaatccgcaa agcgcgggca accggcgcat tgagattatg
     6121 gtgctgacca aaagtgcgtc cgatacgctg tatcaatact ttggtcagca tggggataaa
     6181 gtggtgcagc cgctggtgca aaagctggat aagcagcagg tgctttcgca gcgaacgcgt
     6241 taaatgctga atctttacgc atttctcaaa ccctgaaatc actgtatact ttaccagtgt
     6301 tgagaggtga gtaatgcgta aaatcattca tgtggatatg gactgctttt tcgcagcggt
     6361 ggagatgcgc gacaatcccg ccctgcgcga tatccctatt gctattggcg gcagccgcga
     6421 acgtcggggg gtgatcagca ccgccaatta tcccgcgcgt aaatttggcg tacgtagcgc
     6481 tatgccgaca gggatggcgc tcaaattatg cccgcatctc accttgcttc cggggcgctt
     6541 tgacgcctac aaagaagcct caaatcatat ccgtgaaatc ttctcgcgct acacctcgcg
     6601 tattgaaccg ttgtcactgg atgaggctta tctcgacgtc accgatagcg tccattgcca
     6661 cggttctgcg accctcatcg cccaggaaat ccgccagacg attttcagcg agctgcaact
     6721 gacagcgtct gcgggcgtgg caccagtaaa gtttctcgcc aaaatcgcct ccgacatgaa
     6781 taaacccaac ggtcagtttg tgattacgcc ggcagaagtt ccggcatttt tacaaacctt
     6841 accgctggca aaaatccccg gcgtcggcaa agtctcggcg gcaaaactgg aagcgatggg
     6901 gctgcggacc tgcggtgatg tacaaaagtg tgatctggtg atgctgctta aacgctttgg
     6961 caaatttggc cgcattttgt gggagcgtag tcaggggatt gacgagcgcg acgttaacag
     7021 cgaacggtta cgaaaatccg tcggcgtgga acgcacgatg gcggaagata ttcatcactg
     7081 gtctgaatgt gaagcgatta tcgagcggct gtatccggaa cttgaacgcc gtctggcaaa
     7141 ggtaaaacct gatttactga tcgcccgtca gggagtgaaa ttaaagtttg atgattttca
     7201 gcaaaccact caggagcacg tctggccgcg gctgaataaa gctgacttaa tcgccaccgc
     7261 gcgtaaaacc tgggatgaac gccgcggcgg gcgcggtgtg cgactggtgg ggctgcatgt
     7321 gacgttgctt gacccgcaaa tggaaagaca actggtgctg ggattatgaa taacatacaa
     7381 ataagagact atcagcctgg cgattttcag caactatgcg ctattttcat tagagcggtt
     7441 acgatgactg ccagtcagca ttattcacca caacaaattg ccgcctgggc gcagattgac
     7501 gaatctcgct ggaaggagaa actcgcgaaa tcacaagtgc gggttgcagt cattaatgca
     7561 aaactggtcg gctttattac ctgcgttgaa cactatatcg atatgttatt tgttgaccct
     7621 gaatacaccc gccgtggggt tgccagcgct ttgttaaaac ctttgattaa gtctgaatcc
     7681 gaacttacgg tggacgcaag cataaccgca aaaccctttt ttgaacgtta tggttttcag
     7741 acagttaagc agcaacgcgt tgaatgtcgg ggagagtggt ttattaattt ttatatgcgt
     7801 tataaacagc aacattaaat ccagcttgca atgaaaataa cgcaagccta gtatgtgcgc
     7861 gttttcttat tccacaaact gcaaggaggt aaatcatgac aaatccttta tcaatgactc
     7921 tttgcagacc tttccaggat taattctttt tttcttgccc tggattcgtc tgccatttcc
     7981 tgatttttta attgttactc tcaataattc aagtcgcaac acatgcaact tgaagtatga
     8041 cgagtctatt tacggaatgg tatatgggca attatattcg tcccttatcc gatgcggtat
     8101 ttaccatcgc atctgatgac ctgtggatcg agagtttagc gatccaacaa ttacacacca
     8161 cggcaaattt acccaacatg cagcgcgtag tggggatgcc agatttacat cccggacgcg
     8221 gctatccgat tggcgcagcg ttcttctctg ttggtcgttt ttatccggca ctggtcggca
     8281 atgatatcgg ctgcggtatg gcgctatggc aaacagatat tctcgctcgc aaatacaacg
     8341 ccgataagtt tgaaaagcga ttatcagatc tggatgacgt tgctgaagaa agctggctgg
     8401 aggaaaacct gccgtcagca tttgcacagc atccgtggcg cagctcgctt ggctctatcg
     8461 gtggcggcaa tcatttcgct gaactgcaac aagttgatca aatcatcaac gctgaactgt
     8521 ttgcactggc aggtctggat gcacaacatc tgcaactgtt ggttcatagc ggctcgcggg
     8581 gggtggggca gtctatttta cagcggcata ttgcctcgtt ctcgcatcat ggtttgcctc
     8641 aaggcagtga cgacgcgcta cggtatattg cggaacatga cgatgcgctg gcgtttgcgt
     8701 gtattaatcg ccagatgatc gctttgcgca taatgcaaca agttaaggcc acgggaagtc
     8761 cggttctgga tgtggcgcat aactttgtta gcgcgtgtca aatcggcgat caacagggct
     8821 ggttgcatcg taaaggtgcc acgccggatg acaacggtct ggtgattatt cccggttcac
     8881 gtggtgatta ctcctggatt gtacagcctg ttgcgaacga aaaaacgctc cactcgctgg
     8941 cgcatggggc agggcgtaaa tgggggcgca ccgagtgtaa agggcgtctg gcagcgaaat
     9001 acacggcgac gcaactctcg cgtactgaac ttggcagtcg ggtaatttgt cgcgataaac
     9061 aactcatctt tgaagaagcg ccacaagcct ataaatcggc tgaaagcgtg gtgcaatgtc
     9121 tggtgcaggc cggtttaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa
     9181 acagtggagg gaaaaaagga tgatcttgct acaactctcc tcagcgcagg ggccggaaga
     9241 atgttgtctc gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga
     9301 cgtcgcggta acggtgctgg aaacagaaac gggccactac tctgacacgc tgcgttcggc
     9361 gctgatttct ctggatggcg ataacgcatg ggcattaagc gaaagctggt gtggcactat
     9421 tcagtggatt tgtccgagtc cgtatcggcc tcatcatggg cgcaaaaact ggtttctggg
     9481 cattgggcgt tttaccgctg atgagcagga acaatcggat gcaatccgtt atgagacgtt
     9541 gcgttcgtcg gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac
     9601 gcatttggca tccggtatta gcgtgaaggt tcagtcagag cgtagtcagc atgctaacaa
     9661 gcggctggca cgattgctga ttgcctggaa gctggagcag cagcaacagg aaaatagcgc
     9721 ggtgctgaaa tcgctgcggc gaatgttcca tcaccagatt gaacgtggca acccgagacg
     9781 gacatttaca gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga
     9841 ttaaacgcca tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc
     9901 agtcagcagc gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg
     9961 tggaccggtg atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa
    10021 cagaccacat tccaggcccg cgtggataat ctggatgttc ggtgttttgt tgaacagacg
    10081 ctgataggtt tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg
    10141 atatgcgcct ttcgcttcgg ttttcgcgcc agctagttta cccagcgaat ccagcatgct
    10201 caccacgtag tctttaccgc tgtcgatcag tgaacggatc aggcagtgaa tttctacgtt
    10261 attgtcagtc atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac
    10321 gtcggaattg cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg
    10381 agatttcgca atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt
    10441 tttctctttc tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt
    10501 caggacgtcg actttatcag ctgcgacagc aatggtcgca aaggcttcac gcgggatggc
    10561 gttacgcagt gtgccgccgt tgaaatcgat aaggcgcaga tccagttctt ccgcatgacc
    10621 cgccaggaag cgcaccagta gtttgttggc attacccagg ccaacgtgga tttcaccgcc
    10681 ggagtgaccg cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac
    10741 cgcttcacga tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta
    10801 gatttcacct tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct
    10861 ctgtaagccg aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag
    10921 cgggccgtga accacgtttt cgtcagccag aaccgccagc gcagacgcca taccaatgcc
    10981 gttatctgca cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg
    11041 gataggatct ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag
    11101 gtgggcctgt aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat
    11161 caggatatta cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac
    11221 aatgtattca gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc
    11281 aaaaatatcc cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct
    11341 tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca
    11401 caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca
    11461 gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata
    11521 atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga
    11581 cacttcacat gagcgaaaaa tacatcgtca cttgggacat gttgcagatc catgcacgta
    11641 aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg
    11701 gcggtctggt accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg
    11761 tttgtatttc cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag
    11821 aaggcgatgg cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgctg
    11881 ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg
    11941 gtcgtccgct ggttgatgac tatgttgttg atatcccgca agatacctgg atcgaacagc
    12001 cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc
    12061 tggcactgcc gggcgttgtt ctttttaact tcaggcaggt tacaatagtt tccagtaagt
    12121 attctggagg ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc
    12181 tttaaacatc ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct
    12241 gtgcagtcgg ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg
    12301 atgtggatct ggcgcggtat tgacccacgt gaaatcctcg acgtccaggc acgtattgtg
    12361 atgagcgatg ctgaacgtac cgacgatgat ttatacgata ctgtgattgg ctaccgtggt
    12421 ggcaactgga tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa agcctgtgcg
    12481 gaagaagatc cgcaactcag tggtcgacac tggctgcatg cggctacgtt gtacaacatt
    12541 gccgcctatc ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttatcaaac
    12601 cgcgcctatg aagaggccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc
    12661 gtacccggcg gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc
    12721 ccgacagtat taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat
    12781 gaacgttatt ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc atcggtgggc
    12841 ttttcttcaa aatggaagct cacccaggac tccagcctgt tgcatcagca cgtcttaaag
    12901 gcgctgccta acgtaccgtg ggtggatcac actcgcgtcg cggcctttgg tttccgtttc
    12961 ggcgctaacg ttgccgtgcg tctggcatat cttgaatcgc cgcgtctgaa agcggttgcc
    13021 tgtcttggtc cggtagttca taccctgttg agtgatttta agtgccagca acaggtgccg
    13081 gaaatgtatc ttgacgttct ggcgagtcgt ttggggatgc atgatgcttc cgatgacgcg
    13141 ttacgcgtgg agctgaatcg ctattcatta aaagtgcaag gattgctggg gcgtcgctgc
    13201 ccaacgccaa tgttatcagg ctactggaag aacgatccgt tcagcccgga agaggactca
    13261 cgcttaatca cctcatcatc tgctgacggt aaattattag agatcccatt taacccggtg
    13321 tatcggaatt ttgacaaagg tcttcaggaa atcaccggct ggatcgaaaa acgcttgtgt
    13381 taaaaatttg ctaaattttg ccaatttggt aaaacagttg catcacaaca ggagatagca
    13441 atgacgttac cgagtggaca cccgaagagc agattgatca aaaaatttac cgcactaggc
    13501 ccgtatattc gtgaaggtaa gtgcgaagat aatcgattct ttttcgattg tctggctgta
    13561 tgcgtcaacg tgaaaccggc accggaagtg cgtgagttct ggggctggtg gatggagctt
    13621 gaagcgcagg aatcccgttt tacctacagt taccagtttg gtctgttcga taaagcaggc
    13681 gactggaaga gtgttccggt aaaagacact gaagtggttg aacgactgga gcacaccctg
    13741 cgtgagtttc acgagaagct gcgtgaactg ctgacgacgc tgaatctgaa gctggaaccg
    13801 gcggatgatt ttcgtgacga gccggtgaag ttaacggcgt gagtgaaatg tgccggatgc
    13861 atcacatccg gcaataatca ttaaaactga taggtcatgc caaccgcgac aatatcatca
    13921 ttattaatat tcaatttgtt atcgctatcc agttggttga ttttataatc aacaaacgct
    13981 gacatatttt tgttgaaata atatgtagcc ccgacatcga tataattgac cagatcttca
    14041 tcaccgatac cttcaatatc tttccctttc gataagacat aacccagcga tggacgcaga
    14101 ccaaagtcaa actggtattg agcgaccgct tcaaagttct gtgtcttatt ggcaaagccg
    14161 ccagttattg gcgtcatttt gcgtgtttca gaataaaaag ttgccagata aatattattg
    14221 gcatcgtatt tcagacctgt agcccatgct tctgcacgct tgcctgtgcc acggctttgc
    14281 aggttctgct cgttggtgcg atctgagttg gtataggccc cactaatggc gaaatcgctg
    14341 ccgccaaagt catatgtcaa tgacgtgccg aagccatcgc cgttttgctt tttaacgtcg
    14401 cggttttcgt ttttcccttg atattgcagg gttaagttca ggccatcgat aacgccgaag
    14461 aagtcggtgt tccgatacgt cgccagaccg ctggcgcgtt tggtcataaa gttgtcggtc
    14521 tgcgccgagg aatcgccacc aaattccggg aacatatcgg tccaggcttc cacgtcatac
    14581 aacgcgccca ggttacgacc ataatcgaaa gaacccaaat ctttatattt caacccggca
    14641 aaagcgagac gcgttttttg ctgtgcggta tcactctccg ctttatttcc ggcaaactcc
    14701 gcttcccacc gaccataacc agtcagttga tcgttaattt gtgtttcacc tttaaaacca
    14761 aaacggatat aactctggtc gccatcttta ctggcgttat cactcatata atgcatggct
    14821 ttaactttgc catagacatc cagtttatta ccgtctttat tatatatttc tgcagcctgt
    14881 acagatgcag atgccacaat gcccatcacc actaatgcca gagtgctctt tttcattttc
    14941 attcctgatt ttaattaacg cgcgaatatt cagcgggaga gccccgttga aaacaggaaa
    15001 gtttttaacc tgagattgtt aaagatatat tacag
//
LOCUS       DAZGTR010000534         1786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.guided.325, whole genome shotgun sequence.
ACCESSION   DAZGTR010000534 DAZGTR010000000
VERSION     DAZGTR010000534.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.guided.325"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            101..1060
                     /gene="stxA2"
                     /locus_tag="ACHLCY_005613"
     CDS             101..1060
                     /gene="stxA2"
                     /locus_tag="ACHLCY_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2 subunit A, a/c/d subtype"
                     /protein_id="HFP0372578.1"
                     /translation="MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDV
                     DLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRTVNE
                     ESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK"
     gene            1072..1341
                     /locus_tag="ACHLCY_005614"
     CDS             1072..1341
                     /locus_tag="ACHLCY_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2a subunit B"
                     /protein_id="HFP0372579.1"
                     /translation="MKKMFMAVLFALASVNAMAADCAKGKIEFSKYNEDDTFTVKVDG
                     KEYWTSRWNLQPLLQSAQLTGMTVTIKSSTCESGSGFAEVQFNND"
BASE COUNT          472 a          373 c          439 g          502 t
ORIGIN      
        1 caacgcgcca tatttattta ccaggctcgc ttttgcgggc cttttttata tctgcgccgg
       61 gtctggtgct gattacttca gccaaaagga acacctgtat atgaagtgta tattatttaa
      121 atgggtactg tgcctgttac tgggtttttc ttcggtatcc tattcccggg agtttacgat
      181 agacttttcg acccaacaaa gttatgtctc ttcgttaaat agtatacgga cagagatatc
      241 gacccctctt gaacatatat ctcaggggac cacatcggtg tctgttatta accacacccc
      301 accgggcagt tattttgctg tggatatacg agggcttgat gtctatcagg cgcgttttga
      361 ccatcttcgt ctgattattg agcaaaataa tttatatgtg gccgggttcg ttaatacggc
      421 aacaaatact ttctaccgtt tttcagattt tacacatata tcagtgcccg gtgtgacaac
      481 ggtttccatg acaacggaca gcagttatac cactctgcaa cgtgtcgcag cgctggaacg
      541 ttccggaatg caaatcagtc gtcactcact ggtttcatca tatctggcgt taatggagtt
      601 cagtggtaat acaatgacca gagatgcatc cagagcagtt ctgcgttttg tcactgtcac
      661 agcagaagcc ttacgcttca ggcagataca gagagaattt cgtcaggcac tgtctgaaac
      721 tgctcctgtg tatacgatga cgccgggaga cgtggacctc actctgaact gggggcgaat
      781 cagcaatgtg cttccggagt atcggggaga ggatggtgtc agagtgggga gaatatcctt
      841 taataatata tcagcgatac tggggactgt ggccgttata ctgaattgcc atcatcaggg
      901 ggcgcgttct gttcgcaccg tgaatgaaga gagtcaacca gaatgtcaga taactggcga
      961 caggcccgtt ataaaaataa acaatacatt atgggaaagt aatacagctg cagcgtttct
     1021 gaacagaaag tcacagtttt tatatacaac gggtaaataa aggagttaag catgaagaag
     1081 atgtttatgg cggttttatt tgcattagct tctgttaatg caatggcggc ggattgtgct
     1141 aaaggtaaaa ttgagttttc caagtataat gaggatgaca catttacagt gaaggttgac
     1201 gggaaagaat actggaccag tcgctggaat ctgcaaccgt tactgcaaag tgctcagttg
     1261 acaggaatga ctgtcacaat caaatccagt acctgtgaat caggctccgg atttgctgaa
     1321 gtgcagttta ataatgactg aggcataacc tgattcgtgg tatgtgggta acaagtgtaa
     1381 tctgtgtcac aattcagtca gttgacagtt gcctgtcaga ctgagcattt gttaaaaaaa
     1441 tttcgcatgg tgaatccccc tgtgtggagg ggcgactggt gaaaaatcct tgcttgtgat
     1501 tcattatcga cacgggttcg gtggtaccag gccgaactca ccgggaggca cccggcacca
     1561 tgcagtatac agagattagg catataccaa ggcctctcat agcaggggcc tttttacatg
     1621 taaaaaagcc cgagtgggtt cgggcaattg catgagatac tcgttttaat aatcgaaagc
     1681 attttaacca ggattcataa ggctgcgcaa ctgcgcggcc tttttcgtat ttcgggctgt
     1741 agtcttcctt ctgccattgt cctgtaactt ccggacttca gcccgc
//
LOCUS       DAZGTR010000535         1416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2108H6711
            SAMN43267573-rid23082153.guided.357, whole genome shotgun sequence.
ACCESSION   DAZGTR010000535 DAZGTR010000000
VERSION     DAZGTR010000535.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267573
            Sequence Read Archive: SRR30307392
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350710.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350710.1
            Genome Coverage       :: 76x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 09:39:19
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,617
            CDSs (total)                      :: 5,515
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 102
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 271
            CDSs (without protein)            :: 271
            Pseudo Genes (ambiguous residues) :: 0 of 271
            Pseudo Genes (frameshifted)       :: 127 of 271
            Pseudo Genes (incomplete)         :: 130 of 271
            Pseudo Genes (internal stop)      :: 67 of 271
            Pseudo Genes (multiple problems)  :: 43 of 271
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267573-rid23082153.guided.357"
                     /serotype="O111:H 8"
                     /isolate="2108H6711"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-08"
                     /collected_by="SSI"
                     /note="subtype: stx1a; stx2a"
     gene            3..893
                     /gene="stxA1"
                     /locus_tag="ACHLCY_005615"
     CDS             3..893
                     /gene="stxA1"
                     /locus_tag="ACHLCY_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1 subunit A"
                     /protein_id="HFP0372580.1"
                     /translation="MVAKEFTLDFSTAKTYVDSLNVIRSAIGTPLQTISSGGTSLLMI
                     DSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTGFVNRTNNVFYRFADFSHVT
                     FPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTSYLDLMSHSGTSLTQSVARA
                     MLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAEDVDLTLNWGRLSSVLPDYHG
                     QDSVRVGRISFGSINAILGSVALILNCHHHASRVARMASDEFPSMCPADGRVRGITHN
                     KILWDSSTLGAILMRRTISS"
     gene            903..1172
                     /gene="stxB1a"
                     /locus_tag="ACHLCY_005616"
     CDS             903..1172
                     /gene="stxB1a"
                     /locus_tag="ACHLCY_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP0372581.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            complement(1233..>1416)
                     /locus_tag="ACHLCY_005617"
     CDS             complement(1233..>1416)
                     /locus_tag="ACHLCY_005617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0372582.1"
                     /translation="CAIAWCRVPPGEFGLVPPNPRILAYDHQRDHTVHQSPLRTGGFT
                     MQKFSNTSIIRPATTD"
BASE COUNT          386 a          248 c          359 g          423 t
ORIGIN      
        1 atgtggttgc gaaggaattt accttagact tctcgactgc aaagacgtat gtagattcgc
       61 tgaatgtcat tcgctctgca ataggtactc cattacagac tatttcatca ggaggtacgt
      121 ctttactgat gattgatagt ggcacagggg ataatttgtt tgcagttgat gtcagaggga
      181 tagatccaga ggaagggcgg tttaataatc tacggcttat tgttgaacga aataatttat
      241 atgtgacagg atttgttaac aggacaaata atgtttttta tcgctttgct gatttttcac
      301 atgttacctt tccaggtaca acagcggtta cattgtctgg tgacagtagc tataccacgt
      361 tacagcgtgt tgcagggatc agtcgtacgg ggatgcagat aaatcgccat tcgttgacta
      421 cttcttatct ggatttaatg tcgcatagtg gaacctcact gacgcagtct gtggcaagag
      481 cgatgttacg gtttgttact gtgacagctg aagctttacg ttttcggcaa atacagaggg
      541 gatttcgtac aacactggat gatctcagtg ggcgttctta tgtaatgact gctgaagatg
      601 ttgatcttac attgaactgg ggaaggttga gtagtgtcct gcctgattat catggacaag
      661 actctgttcg tgtaggaaga atttcttttg gaagcattaa tgcaattctg ggaagcgtgg
      721 cattaatact gaattgtcat catcatgcat cgcgagttgc cagaatggca tctgatgagt
      781 ttccttctat gtgtccggca gatggaagag tccgtgggat tacgcacaat aaaatattgt
      841 gggattcatc cactctgggg gcaattctga tgcgcagaac tattagcagt tgagggggta
      901 aaatgaaaaa aacattatta atagctgcat cgctttcatt tttttcagca agtgcgctgg
      961 cgacgcctga ttgtgtaact ggaaaggtgg agtatacaaa atataatgat gacgatacct
     1021 ttacagttaa agtgggtgat aaagaattat ttaccaacag atggaatctt cagtctcttc
     1081 ttctcagtgc gcaaattacg gggatgactg taaccattaa aactaatgcc tgtcataatg
     1141 gagggggatt cagcgaagtt atttttcgtt gacttagaat agctcagtga aaatagcagg
     1201 cggagattca taaatgttaa atacatctca attcagtcag ttgttgccgg tctgataata
     1261 gatgtgttag aaaatttctg catggtgaat ccccctgtgc ggaggggcga ctggtgaacg
     1321 gtatgatctc tttgatgatc gtaagcgaga atacgcgggt ttggtggcac caggccgaac
     1381 tcaccgggag gcacccggca ccatgcaatg gcacat
//
